LOCUS       DASWVF010000001         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_127389, whole genome shotgun sequence.
ACCESSION   DASWVF010000001 DASWVF010000000
VERSION     DASWVF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127389"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..375)
                     /locus_tag="VGD55_00005"
     CDS             complement(85..375)
                     /locus_tag="VGD55_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0868755.1"
                     /translation="MSTLWLVVRDVLIAFIVVMFVRLIFDYVQLFAREWRPRGVVLVA
                     LETCYSVTDPPLKFVHRLLPPLRVGAFSADWTVAWIAVIGTAYVLLSVVGSL"
     gene            complement(390..962)
                     /gene="sepF"
                     /locus_tag="VGD55_00010"
     CDS             complement(390..962)
                     /gene="sepF"
                     /locus_tag="VGD55_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEY0868756.1"
                     /translation="MAVMRKAMVYLGLVEDDAENYDEYDEYEAPAPVAAAVGRRGSSQ
                     ADRSGAEVEYGRRSLSPEMRDRTPAYAGGRSGGGEGRAQSLGAVPMGWADSAAGAVRV
                     TTLHPRTYNEARAIGEHFREGTPVIMNLTEMDDADAKRLVDFAAGLIFGLRGSIERVT
                     AKVFLLSPQNVEVTAEDKQRIAEGGFFNQS"
     gene            complement(1021..>1556)
                     /locus_tag="VGD55_00015"
     CDS             complement(1021..>1556)
                     /locus_tag="VGD55_00015"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0868757.1"
                     /translation="RDQEAVPKIREVNEVAAPVPLLHWHFVGALQTNKCASVVTYASL
                     VHSVDRLRLVSALGRAARDRAVPRVRCLVQVDLDEISRPGRAGARPQDVDALADAIAS
                     QPGLELAGVMAVAPLGAEPAFAALAAIADGVRQRHPSATVISAGMSGDLESAIANGST
                     HVRVGAALLGSRRHPVG"
BASE COUNT          297 a          524 c          494 g          241 t
ORIGIN      
        1 gtcagcggca tcgggtcacc tcgggggttg ttgggtacgc gccggacaaa tcatagggag
       61 tcgttgagag cgttatctgg aatgtcatag ggagccgacc acggacaaca ggacgtaggc
      121 agtgccgatg acggcgatcc atgccacggt ccagtcggca ctgaacgccc ccacacgtag
      181 cggcggcaac agtcggtgca cgaacttcaa cggtgggtcc gtcaccgaat agcacgtctc
      241 caacgccact aagacgaccc cgcgggggcg ccattctcga gcgaaaagct gaacatagtc
      301 gaaaataagg cgaacgaaca tcaccacgat gaaggcaatg aggacgtcgc gcacgaccag
      361 ccacaaagtg ctcatgacgt gccggccgcc tagctttgat tgaagaagcc gccttcggcg
      421 atccgctgct tgtcttccgc ggtcacctcg acgttctgcg gtgagagcag gaacactttc
      481 gcggtcacac gctcgatact gccacgaaga ccaaagatga gcccagcggc gaagtcgaca
      541 agccgcttgg cgtcggcgtc gtccatctcc gtcaggttca tgatgaccgg ggtgccctcg
      601 cggaagtgct caccgatcgc acgtgcctcg ttgtaggtgc gcgggtgcag ggtcgtgacc
      661 cgcacggccc cagcggcgga gtcggcccag cccataggga cggccccgag cgactgcgcc
      721 cggccctcgc cgccgcccga acgcccaccc gcatacgccg gggtgcgatc gcgcatctca
      781 ggcgacaacg agcgacgccc gtattcgacc tctgcgccgg aacgatcagc ttgcgacgag
      841 ccgcggcgcc ccacggcagc ggcaaccggt gcaggggcct cgtactcgtc gtattcgtcg
      901 tagttctcgg cgtcgtcctc gacgaggccg aggtagacca tcgccttacg catgacggcc
      961 atcgctgtct ccctcagtct ccctcagaat ctcccgcaca cacttacgtc cgtcgggacc
     1021 ttacccgaca ggatgccgcc gagagccgag caacgccgcg ccgacacgca cgtgtgtgga
     1081 accgttcgcg atggcggatt cgaggtcacc actcattccg gccgaaatta cggtggccga
     1141 cggatgtcgc tgccggacac cgtcggcgat agcagcgagc gcggcgaagg ccggctcagc
     1201 gcccagcggc gcgacggcca tgacgccggc aagctcgagt cccggctgcg atgcgatcgc
     1261 atcggcgagc gcgtcgacgt cctgcggtcg cgccccggct cggcccggtc tggagatctc
     1321 gtcgagatcg acctggacca gacaccgaac ccgcggcact gcccgatccc gggcggcgcg
     1381 gccgagcgcg ctcaccaacc tgaggcgatc gaccgagtgc acgaggcttg cgtaggtgac
     1441 caccgacgca cacttattgg tctggagcgc gccgacgaaa tgccaatgta gtaggggtac
     1501 aggtgcggcg acctcgttga cttcgcgaat cttcggcact gcctcctggt cgcgac
//
LOCUS       DASWVF010000002         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_127393, whole genome shotgun sequence.
ACCESSION   DASWVF010000002 DASWVF010000000
VERSION     DASWVF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127393"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="VGD55_00020"
     CDS             complement(<1..>1509)
                     /locus_tag="VGD55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0868758.1"
                     /translation="IADLPLGADIIANADEFTLRACQRIGYAGNPLEDGTLSEYRVSA
                     VPLTSMTVEALKEIDISKKEAERAKNMFALGLLSWMYNRPTASTLEFLERKFAKTPEV
                     AKANVTAFRAGYNFGETTETFSVSYEIKPAPMKAGTYRNISGNLALSYGLVAASKATG
                     LSLFLGSYPITPASDILHELSKHKAFGVRTFQAEDEIAGVTAALGAAFGGSLGVTTTS
                     GPGVALKSEAIGLGVMLELPLIIVDVQRGGPSTGLPTKTEQADLLQAMFGRNGEAPVP
                     IVAPSTPSDCYATALEAARIATKYRTPVFLLSDGYLANGSEPWLLPDVELLPDLRVTF
                     ATEPNHIDAKGEPEFWPYLHDEETLARAWAIPGTPGLEHRVGGLEKADGSGDISYDPA
                     NHDLMVRLRQAKVDGIARDIAPLTVDDPSGEADVLVLGWGSTYGPIGAACRQVRARGL
                     KVAQAHLRHLNPFPANTGEVLRAYDRVLIPEMNLGQLALLIRAKFLIDAVSY"
BASE COUNT          271 a          504 c          471 g          263 t
ORIGIN      
        1 tcatacgaca ccgcgtcgat caggaacttc gcccggatga gcaacgccag ctgaccgagg
       61 ttcatctcgg ggatcaggac tcggtcatac gcccgcaaca cttcaccggt gttggccggg
      121 aacgggttca gatgccgcag gtgcgcttgg gcgaccttca ggccacgcgc gcgcacctgc
      181 cggcaggccg caccgatcgg accgtacgtc gaaccccagc cgaggaccaa cacgtcggcc
      241 tcgccggaag gatcgtcgac cgtcagcggc gcgatgtcgc gcgcgatgcc gtcgaccttg
      301 gcctggcgca gtcgcaccat caggtcgtgg tttgccggat cgtaggagat atcgcctgag
      361 ccgtcggcct tttcgaggcc acccacgcga tgctcaagac ccggtgtgcc aggaatcgcc
      421 catgcccgcg cgagggtctc ctcatcatgc aagtacggcc agaactcggg ctcacccttc
      481 gcgtcgatgt ggttcggctc tgtcgcgaac gtgacccgca ggtcgggcaa caactcgacg
      541 tccggcaaga gccatggctc cgaaccgttc gccagataac cgtcggacag caagaagacc
      601 ggcgtccggt acttggtcgc tatacgggcc gcttcgagcg cggtcgcgta gcagtctgac
      661 ggcgtgctcg gcgcgacgat cggcactggg gcttcgccgt tgcgaccaaa catcgcctgc
      721 agcaggtcgg cctgttcggt cttggtcggc agcccggtcg acggaccgcc gcgttgcacg
      781 tcgacgatga tcagcggcaa ctcgagcatc acaccgagcc cgatcgcctc gctcttcaac
      841 gccaccccag gaccagatgt cgtggtcaca ccgagactgc cgccgaacgc tgcaccaagc
      901 gctgccgtca cgccggcgat ctcgtcttca gcttggaacg tccgaactcc gaacgccttg
      961 tgcttcgaga gttcatgcaa gatgtcggac gccggggtga tggggtagga gccaaggaac
     1021 agcgacaacc cggtcgcctt cgacgccgca acgagtccgt acgacaacgc gaggttgccg
     1081 gagatgttgc ggtaggtgcc cgccttcatc ggtgccggtt tgatctcgta cgaaaccgag
     1141 aacgtctcgg tcgtctcgcc gaagttgtag ccggcccgga acgccgtgac gttcgccttc
     1201 gccacctcag gggtcttcgc gaacttgcgc tccaagaact ccagcgtcga tgcggtcggc
     1261 cggttgtaca tccacgacag cagaccgagc gcgaacatgt tcttcgcgcg ttcggcctcc
     1321 ttcttcgaga tgtcgatctc cttgagcgcc tccaccgtca tcgacgtcag cggcaccgcg
     1381 gaaacccgat actcagaaag cgtgccgtcc tcgagcgggt tgccggcgta gccaatgcgc
     1441 tggcacgcac gaagcgtgaa ctcgtcggcg ttcgcgatga tgtcggcgcc tagcggcaga
     1501 tcggcgatg
//
LOCUS       DASWVF010000003         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_76470, whole genome shotgun sequence.
ACCESSION   DASWVF010000003 DASWVF010000000
VERSION     DASWVF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76470"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /gene="pyk"
                     /locus_tag="VGD55_00025"
     CDS             complement(<1..1118)
                     /gene="pyk"
                     /locus_tag="VGD55_00025"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0868759.1"
                     /translation="MSRRAKIVCTLGPATYSAEAIRSLVDAGMDVARLNLSHGTHADH
                     EQVYRLVREASDASGRAVGIMVDLQGPKIRLGRLASGPVTLVAGAEFTITTDDVPGSK
                     VLASTTYEGLPGDVRPGLRILIDDGRVCLEVVAVDGPRVVTTVIEGGTISDHKGINVP
                     GAALSVPAMSEKDVDDLRWGLRIGADMIALSFVRSAEDFNDVAKIMDEEGRRVPVIAK
                     IEKPQAVDHLEEIMEAFDGFMIARGDLGVEMPLEQVPLVQKRAIALARRNAKPVIVAT
                     QMLETMISASRPTRAEVSDCANAVLDGADALMLSGETSVGIHPSLTVATMARIISTVE
                     GDALDTIPVVAGKARTKGGAIARAAADVGADLGARYLVA"
     gene            complement(1163..>1783)
                     /locus_tag="VGD55_00030"
     CDS             complement(1163..>1783)
                     /locus_tag="VGD55_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0868760.1"
                     /translation="ITAAGKHVIIIGGGDTGADCLGTAHRQGAASITQLEILPQPPNS
                     RAEITPWPTWPLMLRTSSAHEEGGERKFAVNTEEFLSDDAGNVRALRVVEVKMSGGAI
                     EAIAGTEFELPAELVLLAMGFVGPERTLAEQFKLDLDTRGNIARDSSYASNMPGIFVA
                     GDAGRGQSLIVWAIAEGRAAAASVDRFLTGVDRLPAPIPPTARPLT"
BASE COUNT          298 a          614 c          565 g          306 t
ORIGIN      
        1 gccaccaggt agcgcgcacc aaggtcggca ccgacatcgg cagccgcccg cgcgatcgcg
       61 ccaccctttg tgcgggcctt gccggccacg accggaatcg tgtcgagggc gtcgccctca
      121 accgtgctga tgatgcgcgc catcgtcgcg acggtcagcg acgggtggat gccgacactc
      181 gtctcccccg acagcatcag cgcgtcagca ccgtcgagca cggcgttcgc gcagtcggac
      241 acctcggctc gcgttggtcg cgatgcgctg atcatcgtct cgagcatctg tgtcgcaacg
      301 atgaccggct tggcgttgcg gcgagcgagc gcgatggccc gcttctgcac gagcggcacc
      361 tgctcgagcg gcatctcgac gccgaggtca ccgcgagcaa tcatgaagcc gtcgaacgcc
      421 tccatgattt cttcgaggtg gtcaaccgcc tgcggcttct cgatttttgc gatgaccggc
      481 actcgccggc cttcctcgtc catgatcttc gcgacgtcgt tgaagtcttc ggccgaccgc
      541 acgaacgaca gcgcgatcat gtcggcaccg atgcgcaaac cccagcgcag gtcgtcgaca
      601 tccttctcac tcatggccgg caccgacagc gcagcgcccg gcacgttgat gcccttgtga
      661 tcggagatcg tgccgccctc gatcaccgtc gtgacaacgc gcgggccgtc aaccgcgacg
      721 acctcgaggc acacccgacc gtcgtcgatc aaaatgcgta aaccagggcg gacgtcgccg
      781 ggcagccctt catacgtggt cgacgccagc actttgctgc cgggaacatc gtctgtggtg
      841 atcgtgaact cggcccctgc gaccaatgtc accggcccac tcgcgaggcg gccgagccgg
      901 atcttcgggc cctgcaggtc gaccatgatg ccgaccgcgc ggccgctggc gtcggaggcc
      961 tcgcgcacga gtcggtagac ctgctcgtgg tcggcatgcg tgccgtgact gagattcagc
     1021 cgcgcgacgt ccatgcccgc gtcgacgaga ctgcggatcg cctcagccga ataggtcgcc
     1081 ggccccaacg tgcagacgat cttcgcccgg cgactcatat gcccagccta gagcgacgcg
     1141 agatcacgaa ggtcgccggg cgttacgtga gtggccgggc ggtcggcggt atcggcgcgg
     1201 gcagccggtc gacacctgtc aggaaccgat cgaccgaggc tgccgctgcg cgaccttctg
     1261 caatcgccca cacgatcagc gactgacctc gccccgcgtc gccagcaaca aagattcccg
     1321 gcatgttgct cgcgtatgac gaatcacgcg cgatgttgcc tcgggtatcg aggtcgagct
     1381 tgaactgctc ggcaagtgtg cgttcgggcc cgacgaagcc catggcgagg agcacgagtt
     1441 cggcgggcag ttcgaattcg gtgcccgcga tcgcctcgat cgctcccccg gacatcttga
     1501 cctcgaccac gcgcaatgcg cgcacatttc cggcatcgtc agagaggaat tcctcagtgt
     1561 tgacagcgaa cttccgctcg ccaccttctt cgtgcgccga cgacgtgcgc agcatcaacg
     1621 gccaggtcgg ccatggcgtg atctcggcgc ggctgttggg cggctgcggc aaaatctcga
     1681 gttgcgtgat cgacgcggca ccttggcgat gcgcggtacc gaggcagtcg gcacccgtgt
     1741 cgccgccgcc gatgatgatg acgtgcttgc ccgcagcggt gat
//
LOCUS       DASWVF010000004         6829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_254812, whole genome shotgun sequence.
ACCESSION   DASWVF010000004 DASWVF010000000
VERSION     DASWVF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254812"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VGD55_00035"
     CDS             complement(<1..699)
                     /locus_tag="VGD55_00035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY0868761.1"
                     /translation="MKAWVVDRCAPIDDGPLRRIDRDAPEAGPGQIRVRVICCGVCRT
                     DLHLAEGDLIPRQDFVTPGHEIVGIVEALGLSATRFDVGDRVGVAWLASTDGSGRFCR
                     RSDENLCESATFTGWDINGGYADVCVADERFVYALPSAISDEQAAPLLCAGIIGYRAL
                     LAANVPVGGRLGLYGFGGSAHLTAQIAMHLGMRVHVLTRGEHNQELARQLGVDSVGEA
                     ADAPPERLDGAILFA"
     gene            complement(733..876)
                     /locus_tag="VGD55_00040"
     CDS             complement(733..876)
                     /locus_tag="VGD55_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868762.1"
                     /translation="MPGIQEPTPQALDDQLHRVADHMGRRFGDRAAIGHPEWPVRPEA
                     LRA"
     gene            complement(981..2183)
                     /gene="chrA"
                     /locus_tag="VGD55_00045"
     CDS             complement(981..2183)
                     /gene="chrA"
                     /locus_tag="VGD55_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY0868763.1"
                     /translation="MTASRATHDGATVPPASLRDIAWEWGRLGCIGFGGPPTHIKLLR
                     DLCVDRRRWVDAGDFEDTVAACNLLPGPASTQLAIFCAWRARGRLGAVVGGIAFIVPG
                     LIVVIALTALFLANSPPRWVLGAGAGAGAAVAAVAVRAGTSLLPASWRRRRNAVRWTT
                     YLTLGAVSAAVLGPWVVVVLLGCGVAELALERFWHADSSSPGTTSLRSIAALPLLMSS
                     AVGTGVLASVVWVAFKVGALSYGGGFVIIPLMQSDAVSHYHWMSSGQFLNVVALGQIT
                     PGPVVQTVAAVGYAAAGIGGALLASVVAFGPSFAFVLLGARRFDALRANPRARAFLTG
                     AGPAAIGAILGSSIPLTRAISEAWQYGVLAGAAVLLLALRRSVVLTLLCAGAAGTIVA
                     LAGGPIPH"
     gene            2412..3119
                     /locus_tag="VGD55_00050"
     CDS             2412..3119
                     /locus_tag="VGD55_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY0868764.1"
                     /translation="MTDWDEWRALLGATAGSRAQPEQICHLCTQRLGISGAGLSMVTP
                     TGARGIVCSTDEVSARIEDLQFTLGEGPCFDAARSGSPILISDLDETDDLAIERWPTF
                     LAGAIAAGARALFVFPLRVGAISVGALDLYRDRVGDLTADELPAALMAAAAAAIALLR
                     FEVGRAAIFSDNPDAMVGYQFQVHQATGMIQVQLGVSTDEALVRLRARAFALGRPLVA
                     VATDVVERTIRFTAEDE"
     gene            3116..3904
                     /locus_tag="VGD55_00055"
     CDS             3116..3904
                     /locus_tag="VGD55_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY0868765.1"
                     /translation="MTDKQNSRRVAAGTSREQALANTFIALADTLVDDYDIVELLDQL
                     VNACVNLLGVSAAGLLLDDQKGNLAVVASSSEETRLLEVFQLQKDEGPCLDCVRSGVS
                     VSYADMRAELARWPLFVPAAVAAGVLSVTAVPLRLRDQTIGGLNLFDDVAEAVPPGDR
                     RMAQALADMATIGILHRRSAHRSTVVAEQLQHALNSRIVIEQAKGVLAERSNMTMERA
                     FEVLRRHARNNNLKLTDVALAVVRGELFVSGTGTAAGGGAGPQA"
     gene            4043..4264
                     /locus_tag="VGD55_00060"
     CDS             4043..4264
                     /locus_tag="VGD55_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868766.1"
                     /translation="MTPTTTERHQLKRTLREQVETVPSERAHPFQEWLQAIADLTRAE
                     ARGADDAERERLSAEVGRARGAVEFSLGQ"
     gene            complement(4278..4496)
                     /locus_tag="VGD55_00065"
     CDS             complement(4278..4496)
                     /locus_tag="VGD55_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868767.1"
                     /translation="MITRLRHFGAFWYDFVIGDDWQVAAGVVVALAATYGLHRAAVPA
                     WWALPVAVLLLLPYSLWRATKPKPPDAE"
     gene            complement(4493..5266)
                     /locus_tag="VGD55_00070"
     CDS             complement(4493..5266)
                     /locus_tag="VGD55_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868768.1"
                     /translation="MSGGALFLAVFLACAVEAVEATTIVLAAGTARDWRSATTGLFAA
                     LVTLAVLIAALGPALSAVPLHALRLIVGGLLLVFGLQWLRKAILRASGHKAQHDEEQI
                     YATELAAAQAAPHERRGFVTDWYAFTLSFKGVVLEGLEVVFIALTFGTNDHNVPLASA
                     AAGAAVLVVAAVGVAVRAPLARVPENSMKFVVGVMLTAFGTFWGAEGAGARWPGDDAS
                     LPVVVAAVAVLAFGLVAFLRRARRDPRPSPIPALSVSNR"
     gene            complement(5292..>6829)
                     /locus_tag="VGD55_00075"
     CDS             complement(5292..>6829)
                     /locus_tag="VGD55_00075"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY0868769.1"
                     /translation="GTEGFDYRDGQVWGIHLGWSGDQRYLAQRLNTGVVLLGAGEILA
                     AGEVILGAGESITTPWAYGVYSGAGIDGASRRLHAMLRSRPSHPRRPRPIVLNTWEAV
                     YFDHSFDRLAALADVAAQIGVERFVIDDGWFGSRRDDHSGLGDWHVSAQVWPAGLGPI
                     ADHVRSRGMEFGLWFEPEMVNLDSDIARAHPEWVIGTPGRMPPPSRGQQVLDVSHPDA
                     FSYLLERLSSLVGAYGISYIKWDHNRDIADPVHRGGPRAGRPAIHDETLATYRLMAQL
                     RDLHPGLEIESCSSGGSRIDFGVLEHTDRVWASDCIDPYERVRIVSGISTLLPFELIG
                     AHVASGRNHTTGRTHGLSFRLAVALFGHAGLEWDLTTTTPEVREQLSVWVTFAKSVRQ
                     LLRTGELVRVARPADHETALYGVVSPRRDEALFSLVRLQTGPRYGTAPVVFAGLDPGL
                     RYHLRRVAMPGEEPPVHGQARSHDGVSDIVLPGALLMGAGVEAPNLQPERAAIYHLQA
                     QPG"
BASE COUNT         1252 a         2357 c         2182 g         1038 t
ORIGIN      
        1 cgcgaaaagg atcgcgccgt caaggcgctc cggcggtgcg tccgcagctt cgccgaccga
       61 gtcgacgccg agctgacgcg cgagttcttg gttgtgctcg ccacgtgtaa gcacgtgcac
      121 gcgcatgccg agatgcatgg cgatctgcgc tgtcagatgt gcgctgccac cgaaaccgta
      181 gagaccgagc cggccaccaa ccggtacgtt cgcagccagc aaagcgcggt atccgatgat
      241 gcccgcgcac agcaatggcg cggcctgctc gtcactgatt gctgagggca gtgcgtacac
      301 gaagcgctcg tcggccacac acacgtctgc gtagccgccg ttgatatccc acccagtgaa
      361 ggttgctgat tcgcacagat tctcgtcgct tcgccggcag aatctgccgc tgccatcggt
      421 actggccaac cacgcgacac cgacacgatc gccgacgtcg aatcgagtgg ccgaaagccc
      481 gagggcctcg acgatgccga cgatttcgtg accaggtgtc acgaaatcct gccgcggaat
      541 cagatcgccc tcagccaggt gcaggtcggt gcggcagaca ccgcaacaga tcactcgtac
      601 ccggatctgt cccgggccgg cttcgggcgc atcgcgatcg atgcgtcgca acggcccgtc
      661 atcgatcggt gcgcagcggt cgacgaccca ggccttcacc ccgtcatcat ctcgcggttt
      721 catcgaccgg cgtcaggctc tcaacgcctc aggtcgcacc ggccattcgg ggtggccgat
      781 ggcagcgcga tcaccgaacc tgcgccccat gtgatcggcg acccggtgca gctggtcgtc
      841 gagtgcttgc ggggtgggtt cttgaatgcc tggcatctcg gggtctcctc gggtctttaa
      901 cgacggcgtg cactcgtcgc atcacctccc actactttcg ggcccgcgtt cacccgggtg
      961 ttcacgtcga ggccggacac ttagtgcggt atcggaccac cggcgagggc gacgatcgtc
     1021 cccgcggctc cagcacacaa cagggtcagg acgacgctgc gtcgcaatgc aagaagcaaa
     1081 accgcagcac cagcgaggac gccgtattgc caggcttcgc tgatcgctcg ggtcagcggg
     1141 atcgaagaac cgagaatcgc accgatcgcg gctggccccg caccggtgag gaatgctcgt
     1201 gcgcgcgggt tggcacgcaa cgcatcgaac cgacgcgccc cgagcagtac gaaagcgaac
     1261 gacggcccga aagcgacaac tgacgcgagc aacgcaccgc cgatccctgc agctgcgtag
     1321 ccaacggcag cgactgtttg cacgacggga ccaggagtga tctgaccaag tgccaccacg
     1381 ttgaggaact gacctgagct catccagtgg tagtgcgaga cagcatcgct ttgcatcagc
     1441 gggatgatga cgaacccacc gccgtacgac aacgcaccga ccttgaacgc cacccacact
     1501 acggatgcga gaacaccggt gccgacggcg ctgctcataa ggagcggcaa ggccgcgatg
     1561 ctgcgcaatg aggtggttcc tggcgacgaa ctgtcggcgt gccagaaccg ctcgagcgcc
     1621 aactccgcca caccacaccc cagcaggacg acgacgaccc acggcccgag cacggcagcg
     1681 gacactgcgc cgagggtgag gtaagtggtc cacctcacgg cgttgcgacg gcgccgccag
     1741 ctggcaggca gcaggctcgt tccggcccgg acggcgacgg ctgcgacggc ggcacctgct
     1801 cccgccccgg cgcccagtac ccaacggggt ggggagttcg cgaggaacag tgccgtcaat
     1861 gcgatgacga cgataagtcc gggcacgatg aacgcaatac cgccgacgac ggcacccaga
     1921 cgtccccgcg cccgccacgc gcaaaagatc gccagctgcg tcgatgcagg accggggagc
     1981 agattacaag ccgctacggt gtcctcgaaa tcgccagcgt cgacccaccg ccgccgatcg
     2041 acgcacaggt cgcgtagcaa cttgatatgc gtcggcggcc cgccgaagcc gatgcagcca
     2101 agccggcccc attcccacgc gatatctcgc agcgacgcag gtggcacggt ggcgccgtca
     2161 tgcgtggccc tcgacgccgt cacgacgcac atctttcctg ttcctgcggg cgctggccgc
     2221 gctatctagt cgcgcccgcc tcgatcaggc tccggacgtg cgagactggc gcctcttggg
     2281 tgtcggtctt gtccttgctc gccaccagtc gcgatccgtg cggaaggcgg caaggacgac
     2341 catggtcacc aaaacgatca cgtctggcgc gcggcccacc gccgccgtcc ccctcgttcc
     2401 tggtatcggc tatgactgac tgggacgagt ggcgcgctct cctcggggca accgccgggt
     2461 cccgggcgca acccgagcaa atctgccacc tgtgcacgca acgactcggc atcagcggtg
     2521 ccgggctgtc gatggtcaca cccacggggg ctcgcgggat cgtgtgttcg accgacgagg
     2581 tgtcggcgcg tatcgaggac cttcagttca cgctcggcga aggcccgtgt ttcgacgcgg
     2641 cgcggtcagg tagcccgatc ctgatctccg acctggacga aaccgatgac ctggcgatcg
     2701 aacgctggcc gaccttcctg gccggtgcga tcgcggctgg cgcccgggca ctattcgtct
     2761 tccccctgcg cgtcggcgcc atcagcgtcg gcgccctcga cctctatcgc gaccgggtcg
     2821 gcgacttgac cgccgacgag ctccccgccg cgttgatggc agccgccgca gcagcaatcg
     2881 cacttttgcg tttcgaggtc ggtcgagctg cgatattctc ggacaatcct gacgccatgg
     2941 tcgggtatca attccaggtc catcaagcga ctggaatgat ccaagttcaa ctcggggtaa
     3001 gcacggatga agcactcgtg aggttgcgcg cgcgggcgtt cgccctcggt cgacccttgg
     3061 tcgctgtcgc gacggacgtc gtcgaacgaa cgatacggtt cacggcggag gacgaatgac
     3121 ggacaaacag aacagccgcc gcgtcgcggc cggaacctcc cgcgagcaag cgctcgccaa
     3181 taccttcatt gctctcgccg ataccctcgt cgacgattac gacatcgtcg aactgctcga
     3241 ccagctcgtc aacgcgtgcg tgaacctgtt gggagtttcc gccgccgggc tgttgctcga
     3301 cgaccagaag ggcaacctcg ccgtcgtcgc ttcctcaagt gaggaaaccc ggcttcttga
     3361 ggttttccaa ctgcaaaagg acgaaggtcc ttgcctggat tgcgtgcgaa gcggggtctc
     3421 cgtcagctac gccgacatgc gcgccgaact cgcgcgctgg ccgctgttcg tgcccgccgc
     3481 cgtcgccgct ggggtccttt cggtcacggc tgtaccgttg cggttgcgtg atcaaacgat
     3541 cggcggtctg aaccttttcg acgacgtagc cgaggcggtg ccgcctggtg atcggcggat
     3601 ggcccaggcg ttggccgaca tggcgacgat cgggatcttg caccggcgtt cggcccaccg
     3661 cagcacggtc gtcgccgaac agctgcagca cgcgctcaac agccgcatcg tgatcgagca
     3721 ggcgaaaggt gtgctcgcgg agcgaagcaa catgacgatg gagcgcgcct tcgaggtgct
     3781 acgtcggcat gcccgcaaca acaacctcaa gctcaccgac gtcgcgctcg ccgtcgttcg
     3841 gggcgaattg ttcgtcagcg ggacagggac cgcggccggc ggcggtgctg gcccacaggc
     3901 ctaaagcccc cttcaactac tccggggcgt atttcagcct aaatagggct agctttggcg
     3961 aacgggtctg gtcgccgccc gcgcaccgtc gccgttgctc ccctgcctcg gcgcttcccc
     4021 gacgacgtgg gcaggtccga cgatgactcc gacaacaaca gaacggcacc aactcaagcg
     4081 cacgttgcgc gagcaggtag aaaccgtgcc ctcggagcgc gcgcacccgt tccaggaatg
     4141 gctccaagcg atcgctgatc tgacgcgggc cgaggcgcgc ggggcggacg acgcggaacg
     4201 tgagcgcctg tcagccgagg tcggccgagc tcgaggcgcg gtcgagttca gcctcgggca
     4261 ataggccccg gcgcgcgtta ctcggcatcc ggcggtttcg gtttcgtcgc gcgccacaag
     4321 ctgtacggca atagcagcag gacggcgacc ggcagcgccc accatgccgg taccgccgcg
     4381 cggtgcaatc cgtaggttgc ggcgagggcg acgacgactc ctgcagccac ctgccagtcg
     4441 tcgccgatca cgaagtcgta ccagaacgcc ccgaagtggc gcagtcgagt gatcaccggt
     4501 tgctcaccga cagcgcgggt atcggcgatg ggcgcgggtc tcgacgtgcg cgccgcagga
     4561 acgcgacgag gccgaacgcc agcactgcga cagccgcgac gacgaccgga agtgaggcat
     4621 cgtcccccgg ccatcgagca cccgctccct cggcacccca gaaggtgccg aacgccgtaa
     4681 gcatgacgcc gaccacgaac ttcatcgagt tctctggcac cctcgcgagt ggcgcgcgca
     4741 ccgcaactcc gacggccgcg acaacgagca ccgctgcgcc agcggccgcc gacgccagcg
     4801 gcacattgtg atcgttggtg ccgaacgtca gcgcgataaa cacgacttcg aggccctcga
     4861 gtacgacgcc cttgaacgac aaggtgaacg cgtaccagtc ggtgacgaag ccgcgacgtt
     4921 cgtgcggcgc cgcttgcgca gccgcgagtt cggtcgcgta gatctgctcc tcgtcgtgct
     4981 gggccttgtg gccgctggct cgcaggatcg ctttgcgcaa ccactgcaac ccgaaaacca
     5041 acagcaaccc gccgacgatc aaccgcaacg catgcagcgg gacggcggaa agcgccggcc
     5101 cgagcgccgc gatcagcacc gctagcgtga ccagcgcggc gaacagtccc gtggtcgcag
     5161 aacgccagtc gcgcgcggtg ccggcggcga gcacgatcgt ggtcgcttcg accgcctcga
     5221 ccgcgcaggc caggaagacc gcgaggaaga gcgcaccgcc gctcaccggg acatcacgaa
     5281 cccagtgtca cctatccggg ctgtgcctgc aggtgataga tcgcggcccg ctcgggctgc
     5341 agattcggcg cctcgactcc cgcacccatg aggagtgcgc cgggaagcac gatgtcgctc
     5401 acgccatcgt ggctcctggc ttgcccgtgc accggaggct cctcaccagg catcgccact
     5461 cgtcgcaggt ggtagcgaag gcccggatca agcccggcga acacgaccgg cgccgtgccg
     5521 tagcgcggac ctgtctgcag ccgaacgagg ctgaacagcg cctcgtccct cctcggactc
     5581 acgacgccgt acaacgccgt ctcgtggtcg gcaggccgag cgacccgcac aagttcgccc
     5641 gtgcgcaata gctggcgtac tgacttggcg aaggtgaccc agacgctgag ttgttcgcgc
     5701 acttccggcg tcgtcgtcgt gaggtcccac tcaagcccgg catgcccaaa cagcgccacc
     5761 gccagccgga acgacaaccc gtgagtgcga cccgtcgtgt ggttgcggcc ggacgccaca
     5821 tgcgcaccga tcagctcgaa cggcaacaac gtcgagatgc ccgacacgat gcgaactcgc
     5881 tcgtacggat cgatgcagtc actggcccac acccggtcgg tgtgctcgag cacaccgaaa
     5941 tcgatgcgcg agccaccgga ggaacacgac tcgatctcaa ggcccgggtg caggtcgcgc
     6001 agttgcgcca tcagccggta agtggccagc gtctcatcat ggatcgctgg ccgaccggcg
     6061 cgcggtccgc cgcgatgcac gggatcggcg atgtcgcgat tgtggtccca cttgatatag
     6121 ctgatgccgt acgcgccaac cagcgacgac agacgttcga gcaggtagct gaacgcgtcg
     6181 gggtgtgaca cgtcgagcac ctgctggccg cgagatggcg gcggcatccg accaggcgtc
     6241 ccgatgaccc actccggatg cgcacgagcg atgtcggagt cgaggttgac catctcgggt
     6301 tcgaaccaga gcccgaactc catgccgcgc gatcgcacgt gatcggcgat cgggccgagg
     6361 ccagcgggcc acacctgcgc cgatacgtgc caatcgccaa ggccggagtg atcgtcacgc
     6421 cgcgacccga accacccgtc gtcgatcacg aaacgctcga cgccgatctg cgccgcgacg
     6481 tcggcgaggg ccgctagccg gtcgaacgag tggtcgaagt agacggcttc ccaggtgttg
     6541 agcacgatcg gtctcggccg cctcggatgg ctcggccggc tccgcagcat cgcgtgcagc
     6601 cgtcgcgaag cgccgtcgat cccggcaccc gagtagacgc cgtatgccca cggcgtggtg
     6661 atcgattcgc ctgcaccgag gatcacctca ccggcggcga ggatctcccc cgcgccgagc
     6721 agcaccacac cggtgttcaa ccgctgcgcg aggtagcgct gatcgcccga ccagccgagg
     6781 tgaatgcccc atacctggcc gtcgcgatag tcgaaaccct cagtgccca
//
LOCUS       DASWVF010000005         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_458611, whole genome shotgun sequence.
ACCESSION   DASWVF010000005 DASWVF010000000
VERSION     DASWVF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458611"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGD55_00080"
     CDS             complement(<1..515)
                     /locus_tag="VGD55_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868770.1"
                     /translation="MNDQIHLVMATPCFGGQVSSVYASSIFALQRALRSKSNVDFKMH
                     LRDGDALITRARANLVTLFLDDPQATHLLFVDADIGFTPEQVFRLIESGADVVAGVYP
                     IKRVNWAKARRALEENRSNVEAAALDYVLEVDNPDQVVVVNGFTRVRYAGTGFLMIRR
                     RVFETMCAHPG"
     gene            complement(563..2440)
                     /locus_tag="VGD55_00085"
     CDS             complement(563..2440)
                     /locus_tag="VGD55_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868771.1"
                     /translation="MSINSINYASSLLGQSVRNINNQLSDLSTQLSTGVKSTNYAGMG
                     VNEGFAVAARAQLANISAFGDTMTNVNTIIAAANTALQSLSDTGGQVQSNAAATPQNL
                     TSAGQTIGQQNAQSELSSIVGILNTQVGDRYIFSGTAINTPAVASADDILNGTGTQAG
                     LKQVIAERQQADLGTSGLGRLVISSPTTTQVQVAEDVAGSPFGLKLSSVSSSLTNATV
                     TGPSGSPPAVAVDLTAGNPNSGDQISFNFNLPDGTSESIQLTASSAVPTPTGSFAIGA
                     TPAATTANLNAALNTAISTLANTSLVAASAVEAGDNFFNTDSTATGSVANNQAATPAP
                     ITGATALSGAAGTNSLSASFAAGDTITVNGTPITFVASGATGNQLNVTDSVQTLLSKI
                     DQITGSSTPSTIDGGAITLHTDNAATLSITSSNTTAFAALGFTGTVTAAQPPLRVSGT
                     PLGSATSLVNGSANTVAWYTGNSGPGSARASSTVRVDSSQTVQFGAQANEQAIRQQLQ
                     SIAVYAAVTLPASGTNSPAAVAALSERTAANLTPQIGQQTIQDIQTDFAAAQSAMKDA
                     TARQTQTQTALQNLVDQTEGISQDQVASEILALQNSLQASYQTTSMLSQLSLTKFLPV
                     A"
     gene            complement(2451..>3125)
                     /locus_tag="VGD55_00090"
     CDS             complement(2451..>3125)
                     /locus_tag="VGD55_00090"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY0868772.1"
                     /translation="SQLNTALAANHLQFSNPSPPSSTLRVTDDGTSSVAMNAASVTTT
                     VSSLAGGSPQLPLFTDGNSLYTGAITANGPQLTGLAGRISVNAAVVADPSKLSVYSTS
                     PPTATGDNTRSDFLFSQLTSQTFSYSPSTGLGSAAQPFKGTISNYLQQFISQQGNAST
                     LATQLQQGQSVVVSTLQQKFDSTSAVNIDTEMASLIQVQNTYAANAHIMSVVQSMMTS
                     LLQAQT"
BASE COUNT          527 a          936 c         1060 g          602 t
ORIGIN      
        1 tagccgggat gcgcgcacat cgtttcgaac acgcgacggc ggatcatcag gaagccggtc
       61 ccggcatagc gcacccgggt gaatccgttg acgacgacca cttggtccgg gttgtcgact
      121 tcaagaacgt aatcgagggc ggcggcttcc acgttcgacc ggttctcttc cagcgcccgc
      181 ctcgccttgg cccagttgac ccgcttgatc ggatagacac cggcaacgac gtccgcgccc
      241 gattcgatca ggcggaacac ctgctccggc gtgaagccga tatcggcatc cacgaacaga
      301 agatgggtcg cctggggatc gtcgaggaaa agcgtgacca ggtttgcccg cgcccgcgtg
      361 atcagcgcgt cgccgtcgcg caaatgcatc ttgaaatcga cgttggattt gctgcgcagc
      421 gcgcgctgca aagcaaagat cgaactcgca tagacgctcg acacctgccc gccaaagcag
      481 ggcgtggcca tcacaagatg aatctgatcg ttcatgacag gctccgaagc ggcggccgcc
      541 gcaaaatcta tttcccttcc cgtcacgcta cgggcaggaa cttggtgaga ctcaactgag
      601 atagcatcga ggtcgtctgg taagacgcct gcaggctgtt ctgcagtgcc aggatctcgc
      661 ttgcgacctg gtcctgcgaa atgccctcgg tctgatccac caggttctgc agcgcggtct
      721 gcgtctgtgt ctggcgcgcc gtggcatcct tcatggcgct ttgcgccgcg gcgaagtcgg
      781 tctggatgtc ctggatcgtc tgctggccga tctgcggcgt caaattcgcc gcggtgcgtt
      841 ctgagagcgc tgcgaccgcc gctggcgaat tggtgccgct cgccggcaac gtgacggcgg
      901 catagaccgc gatcgactgc aattgctggc ggatcgcctg ctcattggcc tgcgcgccga
      961 attgcaccgt ctgggaggaa tcgacccgca ccgtcgaact ggcgcgcgcc gatcccggcc
     1021 ccgaattgcc ggtgtaccag gccaccgtgt tggccgaccc gttgaccagg ctggtcgccg
     1081 atccgagcgg cgttccgctg acccgcagcg gcggctgggc tgccgtcacg gtgccggtaa
     1141 agccgagcgc tgcgaacgcg gtggtgttcg aactcgtgat cgacagcgtc gccgcgttat
     1201 cggtgtgcag tgtgatcgca ccgccgtcga tggtcgacgg ggtcgagctg cccgtgatct
     1261 ggtcgatctt gctcaacagc gtctgcacgc tgtcggtgac attgagctgg ttgccggtcg
     1321 cccccgacgc gacaaaggtg atcggcgtgc cgttgacggt gatggtgtcg ccggccgcaa
     1381 aacttgcgga cagcgagttc gtaccggccg cgcccgacag cgccgttgcc cccgtgatcg
     1441 gcgcgggcgt cgcggcctga ttgttggcca cgctgcccgt ggccgtgctg tccgtattga
     1501 agaaattgtc tccggcttcg accgcggacg ccgccaccag cgaggtattg gcgagcgtgc
     1561 tgatcgccgt attcagcgcg gcgttgaggt tcgccgtggt cgccgcgggc gtggcgccga
     1621 tcgcgaaact gccggtcggc gtcggcacgg cgctcgacgc ggtcaactga atggactcgc
     1681 tggtgccgtc gggaaggttg aaattgaagc tgatctgatc gccggagttc ggattgccgg
     1741 ccgtcaggtc gaccgcgacc gccggcggcg agccggaagg gcccgtcacg gtcgcgttgg
     1801 tcagcgatga ggagaccgaa ctgagcttca gcccgaacgg cgaaccggcg acgtcttcgg
     1861 cgacttgcac ctgggttgtc gtgggcgaag agatgacgag ccgcccgagg ccgctggtgc
     1921 cgagatcggc ctgctgccgc tcggcgatga cctgcttcaa cccggcctgg gtgccggtgc
     1981 cgttcaagat gtcgtccgcg gaggcgaccg ccggcgtgtt gatggcagtg ccggagaaga
     2041 tgtagcggtc gccgacctgc gtgttcaaga tgccgacgat cgaggacagt tcagactgcg
     2101 cgttctgctg gccgattgtc tgcccggcgc tggtcaggtt ctgcggggtg gcggccgcat
     2161 tgctctgcac ctgcccgccc gtgtccgaca acgactgcag cgcggtattg gccgcggcaa
     2221 tgatggtatt gacgttggtc atggtgtcgc cgaaggcgga gatgttcgca agctgcgccc
     2281 gcgccgccac tgcgaacccc tcattgacgc ccatgccggc atagttggtc gatttcacgc
     2341 cggtggaaag ctgcgtcgac aggtccgaaa gttgattatt gatgttgcgg acggactggc
     2401 cgagcagcga ggaagcgtaa ttgatactgt tgatcgacat aacgaacgcc ttacgtttga
     2461 gcctgcaaca ggctggtcat catgctctgc acgaccgaca tgatgtgagc attggccgcg
     2521 taggtgtttt gcacctggat caagctcgcc atctcggtgt cgatattgac ggccgaggtc
     2581 gagtcgaatt tctgctgcag cgtggagacg acgacgctct gcccctgctg caattgcgtc
     2641 gcgagcgtcg atgcgttgcc ctgctggctg atgaactgct gcaggtagtt cgagatcgtt
     2701 cccttgaaag gctgcgcggc cgagccgagc ccggtcgagg gcgaatagct gaaggtttgc
     2761 gacgtcagtt gcgagaacaa gaaatccgac cgcgtgttgt caccggtcgc ggtcggcggc
     2821 gaggtcgagt agacgctcag cttcgaggga tcggcgacca ctgccgcgtt gaccgaaatg
     2881 cgtccggcaa ggcccgtgag ctgcggaccg ttggcggtga tcgcgccggt gtagagcgaa
     2941 tttccatccg tgaacaaagg aagctgcggg cttccgccgg ccagcgagga aaccgtcgtg
     3001 gtgacggatg ccgcgttcat cgcgacggag ctggtgccgt catcggtcac gcgcaatgtc
     3061 gagcttggcg gagacggatt ggagaattgc agatggttcg cggccagcgc ggtgttcaac
     3121 tgcga
//
LOCUS       DASWVF010000006         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_178447, whole genome shotgun sequence.
ACCESSION   DASWVF010000006 DASWVF010000000
VERSION     DASWVF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178447"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VGD55_00095"
     CDS             complement(<1..565)
                     /locus_tag="VGD55_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0868773.1"
                     /translation="MPAAASGPSVLPAAVLFDMDGLLIDTEPLWFSVETEILTELGAP
                     WDYSDHASLVGSSLKVASAFVAERAGNGVTAADVAQQLLLRMRARLRHTPVLQPGVVD
                     LIDELDAAGVPRALVSSSFRVLVEAALEGLAPLTFDTTVVGDEVSVAKPHPEPYLLGA
                     AKLGVDPSLCVALEDSPGGAASASAAGC"
     gene            complement(568..>3642)
                     /gene="metH"
                     /locus_tag="VGD55_00100"
     CDS             complement(568..>3642)
                     /gene="metH"
                     /locus_tag="VGD55_00100"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0868774.1"
                     /translation="TTYAVLRDAYQQEAAGLIEGGADAILIETAQDLLQAKAAIIGAR
                     RAQQAAGSVLPIIAQVTVETTGTLLLGSEIGAALTALEPLGIDLIGLNCATGPAEMGE
                     HLRYLARHARIGLSCMPNAGLPVLGQNGASYPLTPVELADAHDVFTREHGLALVGGCC
                     GTTPAHLRLVVERVRGRELPPRHPHPEPGSASLYQTVPFRQDTSYLAVGERTNANGSR
                     VFRDAMLEARYEDCVEVAREATRDGSHLLDLCVDYVGRDGVADMREVASRFATASTLP
                     LMLDSTEPQVIEAGLECLGGRSIINSVNFEDGEGPASRFARVMPIVREHGAAVVALTI
                     DETGQARTPEHKVAIAERLIETLTQTWGMHESDILVDCLTFTLGTGQEESRRDGLATI
                     EAIRELKARHPAVQTILGLSNISFGLKPVVRTVLNSVFLAECTKAGLDAAIVHASKIL
                     PVARIPDEQREVALDLIYDRRREGYDPLGALLDLFEGVDAQDAKASRAEELAALPLSE
                     RLQRRIIDGERVGLQADLDEALQTRPALEIVNDTLLEGMKVVGELFGRGEMQLPFVLQ
                     SAEVMKAAVAYLEPHMEKTDDAGKGTIVLATVKGDVHDIGKNLVDIILSNNGYSVVNL
                     GIKQPVSAIIEAAQENEADAIGMSGLLVKSTVIMKENLEEISTRGLAGKWPVLLGGAA
                     LTRAYVEQDLAEQFAGVVRYARDAFEGLRLMDALMGVKRGVAGAALPPLRQRRVTAVI
                     DRPESLDDDAVRGRSDVATDVPVPTPPFWGDRLVKGIPLADYADLLDEKATFAGRWGL
                     RGVRGGPSYDDLVETEGRPRMRKWLDRIQTEGWLDAGVVYGYFPCVSEGDDLVVLHED
                     GSDRLRFTFPRQRRDKRLCLADYFRSRESGETDVVAFQIVTIGSRVSDATGVLFAENA
                     YRDYLELHGLSVQLAEALAEFWHRRIREEIGASADDSSDVRAVLEHQAYRGSRYSFGY
                     PACPDLEDRAKIVELLHPDRIGVTLSDELQLHPEQSTDALIVHHPEAKYFSA"
BASE COUNT          639 a         1216 c         1177 g          610 t
ORIGIN      
        1 aacacccggc cgcgctcgct gaggcggccc ctcctggtga gtcctcgagg gctacgcaca
       61 gcgacggatc gacgccaagt ttggcggcgc cgagcaggta cggctcgggg tgcggctttg
      121 cgaccgacac ttcatcgccg acgacggtgg tgtcaaatgt cagtggcgcg agcccctcga
      181 gcgcagcttc tacaagcacc cgaaacgacg acgagacaag tgcgcgcggc acacctgccg
      241 catcgagttc gtcgatcaga tcgacgaccc ccggctggag caccggagtg tgccgaagcc
      301 gcgcgcgcat tctcagcaac agttgttgcg cgacgtcggc agccgtcacg ccatttccgg
      361 cccgctcggc aacgaacgct gacgccacct tcaacgagct gccgaccagg ctggcgtggt
      421 cgctgtagtc ccacggcgcg cctagttcgg tgaggatctc ggtctcgacg ctgaaccaca
      481 gcggctcggt gtcgatgagc aaaccatcca tgtcgaacag cacggcagcg ggcagcacgg
      541 aagggcctga tgcggcggcg ggcatgctca cgcgctgaag tacttggctt ctgggtggtg
      601 cacgatcagc gcgtcggtcg actgctcggg gtgcagttgg agctcatcgg agagcgtcac
      661 gccgatacgg tcggggtgca gcaactcgac gatcttcgcg cggtcctcga ggtcggggca
      721 cgcgggatag ccgaacgagt accgcgatcc gcgataggcc tggtgttcga gcaccgcccg
      781 cacgtcagag ctgtcgtcag cactcgcgcc gatctcctcg cggatgcggc ggtgccagaa
      841 ctcggccaac gcctcagcca attgcaccga aaggccgtgc aactccaagt aatcgcgata
      901 cgcattctcg gcaaacaaca caccggtcgc gtcgctcacc cgcgagccga tcgtgacgat
      961 ctggaacgcg acgacgtcgg tctcccccga ctctcgcgac cggaagtaat cggcgaggca
     1021 caaccgcttg tcacggcgct gccgcgggaa ggtaaaccgc agccgatcgg atccatcttc
     1081 gtgaaggacg acgaggtcgt cgccttcgct gacgcacggg aagtatccgt agacgacgcc
     1141 ggcgtcgagc caaccctcgg tctggatccg gtcgagccac tttcgcatgc gcggccggcc
     1201 ctcggtctcg accagatcgt catatgacgg gccgccacga acaccacgca gcccccaacg
     1261 gccggcgaac gtcgccttct cgtcgagcag gtcggcgtag tcggcgagcg ggatgccctt
     1321 gacgaggcgg tcgccccaaa acggtggcgt tggtaccggc acatcggtcg cgacatcgga
     1381 tcggccccga acggcgtcgt cgtcgagaga ctctggccga tcgatcacag ccgtcacgcg
     1441 tcgctgacgc aagggcggca gcgcggctcc tgcgaccccg cgcttcactc ccatcaacgc
     1501 atccatcaag cgcaggccct cgaatgcgtc gcgcgcatac cggacgacgc cggcgaactg
     1561 ttcggcgaga tcctgctcga cgtaagccct cgtcaacgca gcgccaccaa gcagcaccgg
     1621 ccacttgccc gcgagccctc gagtgctgat ctcctcgagg ttttccttca tgatgacggt
     1681 cgacttcacc agcaatcccg acatgccgat cgcgtcggcc tcgttctctt gcgcggcttc
     1741 gatgatggcc gagaccggct gcttgatgcc gagattcacg acgctataac cgttattcga
     1801 caagatgatg tcgacgaggt tcttgccgat gtcgtgtaca tcgcccttca ccgtcgccag
     1861 cacaatcgtg cccttaccgg cgtcgtcggt cttttccatg tgcggctcga ggtaggcgac
     1921 cgccgccttc atgacctcgg ccgactgaag cacaaacggc agttgcatct cgccgcggcc
     1981 gaaaagctcg ccgactacct tcatgccttc gagcaacgtg tcgttgacga tctcgagcgc
     2041 cggcctggtc tgcagcgcct cgtcgagatc ggcctgcaac ccgacgcgtt cgccatcaat
     2101 gatgcgtcgc tgcaaccgtt ccgacagcgg cagtgccgcc aactcctccg cacgcgaggc
     2161 cttggcgtct tgcgcatcaa cgccctcgaa gagatcgagc aacgcaccga gcggatcata
     2221 cccctcacgc cgccggtcgt agatgaggtc aagggccact tcgcgttgct cgtcagggat
     2281 tcgcgcaacc ggcaaaatct tcgacgcgtg cacgatcgcg gcgtcgagcc cggccttcgt
     2341 gcactcagca aggaacaccg agttgagcac ggttcgaacg actggcttca acccgaacga
     2401 aatattcgaa agaccaagaa tcgtctggac ggcggggtgc cgggctttca gctcgcggat
     2461 cgcctcgatc gtcgcaagac catcacggcg actctcctcc tgcccggtac cgagggtgaa
     2521 cgtcaggcag tcgacaagga tgtcgctctc gtgcatgccc caggtttgtg tgagggtctc
     2581 gatcagccgt tcggcgatgg ccaccttgtg ctcgggagtt cgcgcctggc cggtctcgtc
     2641 gatcgtcaaa gcaacaaccg ctgccccgtg ctcacgcaca atcggcatga cgcgcgcgaa
     2701 ccgactcgcc ggaccctcgc cgtcctcgaa gttgacggag ttgatgatcg aacggccacc
     2761 gaggcactca aggccggcct cgatgacttg cggctcggtg gagtcgagca tcaggggcag
     2821 tgttgatgcg gtggcgaagc ggctcgcgac ctcgcgcatg tcggcgacgc catcgcgccc
     2881 gacgtagtcg acgcacaggt cgagcaggtg tgagccgtcg cgggtggcct cgcgcgcgac
     2941 ctcgacgcag tcttcgtagc gagcctcgag catcgcgtcg cgaaagaccc gcgaaccgtt
     3001 cgcgttcgtg cgttcgccga cggcgaggta ggacgtgtct tggcggaagg gcacggtctg
     3061 gtacagcgat gcggagccgg gttctgggtg cggatggcgg gggggcagct cgcggccacg
     3121 cacgcgttcg accaccaacc gcaggtgtgc gggagtcgtg ccgcagcagc cgcccaccag
     3181 tgcgaggccg tgttcgcggg tgaagacgtc atgggcatcg gccagttcga ctggggtcag
     3241 cggatagctg gcgccgtttt ggccgagcac gggcaggccg gcgttgggca tgcaggaaag
     3301 tccgatgcgg gcgtgtctgg cgaggtagcg caggtgctcg cccatctcgg ccggcccggt
     3361 cgcgcagttc aagccgatca ggtcgatgcc gaggggctcg agcgcggtca gggcagcgcc
     3421 gatctccgag ccgagcagca gcgtgccggt cgtctcgacg gtcacctgcg cgatgatcgg
     3481 aagcaccgaa ccagccgcct gctgcgcccg cctggctccg atgatcgccg ccttggcctg
     3541 cagcagatcc tgcgccgtct cgatcaggat ggcgtcggcg ccgccctcga tcaggccggc
     3601 tgcttcctgc tggtaggcat cgcgcagcac ggcgtaggtc gt
//
LOCUS       DASWVF010000007         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_331329, whole genome shotgun sequence.
ACCESSION   DASWVF010000007 DASWVF010000000
VERSION     DASWVF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331329"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VGD55_00105"
     CDS             <1..1025
                     /locus_tag="VGD55_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0868775.1"
                     /translation="YVLRGGERFFERPEVREAVFLLRGAARGDQSGEGSLVDAVRAVL
                     GAMGFSPTPPAGSGAARDRWESLAALVRLAEERSEPPAAAGPDFAGSTAAAGLVAAGL
                     VDFVADLEERASLQHAPIVDGVTLASLHAAKGLEWDAVFLVGLVDGTLPIVYAETPAQ
                     IDEERRLLYVGITRARRQLALSWAAARSPGGRATRRPSRFFDDLRDDAIDGLDSRGAV
                     APGSTLRSPSAASAAAGKRRVGVERCRVCQKLLTSGAARKLGRCVECPGSVDEELFER
                     LRSWRLERSRELKQPAFCVFTDATLTRIAEVRPQTTQELAGIGGVGPAKLTAYAEQVL
                     KLCERT"
     gene            1195..1530
                     /locus_tag="VGD55_00110"
     CDS             1195..1530
                     /locus_tag="VGD55_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868776.1"
                     /translation="MKNVSKSTMDVTFARGLAMSELGSTGATWRPAVFMFTPKFTPMF
                     TPMFTPNLVPSSTLVQAAVQHPYAAATAAFPTVKTTVTAAIKQHLVKTDAGRDNRTTE
                     GPSSRRIPV"
BASE COUNT          299 a          624 c          574 g          304 t
ORIGIN      
        1 cctacgtctt gcgcggcggt gagcggttct tcgagcgtcc cgaggttcgc gaggcggtgt
       61 tcttgctccg gggcgcggcg cgtggtgacc aatcgggcga gggctcgctc gtcgatgccg
      121 tccgtgcggt gctcggtgcc atggggttca gcccgacgcc gcctgcaggt tcaggcgcgg
      181 ctcgagatcg ctgggagtcg ctcgccgcat tggtccggct cgcagaggag cgaagcgagc
      241 cgcctgccgc tgccggcccc gactttgcgg ggtctaccgc ggctgccggc ctggtcgcgg
      301 ccggcctagt cgacttcgtc gccgacctcg aagaacgcgc ctcgttgcag catgcgccga
      361 tcgtcgacgg cgtcacgttg gcgtcgctgc acgcagccaa gggccttgag tgggacgccg
      421 tcttcttggt tggccttgtc gacggcacgc tgccgatcgt ctatgccgag acgccggccc
      481 agatcgacga agagcgccga ctgctgtacg tcgggattac gcgagcccgc agacagcttg
      541 ccctgtcgtg ggcggcggca cggtctccgg gcgggcgagc cactcgacgt cccagccgct
      601 ttttcgacga cctgcgcgac gatgccatag acggacttga ctcccggggc gctgtcgcgc
      661 cgggcagcac gctccgctcg ccttcagccg cctcggccgc tgctggcaaa cggcgagtcg
      721 gcgtcgagcg atgccgcgtc tgccagaaac tgttgacaag cggcgcagca cgcaagctcg
      781 gccgctgcgt cgagtgcccg ggcagcgtcg acgaagaact attcgagcgg ttgcgaagct
      841 ggcgcctcga gcgctccaga gaactcaagc agcccgcgtt ctgcgtgttc accgacgcca
      901 ccctcacgcg aatcgccgag gtccgcccgc agaccacgca agagcttgcc ggcatcgggg
      961 gcgtcggccc cgccaagctc accgcttacg ccgagcaagt gctcaagctg tgcgagcgaa
     1021 cgtgatgctc cgacggattg aacgtgatgc ccgactgatt gagcgcgacc agaaatctcg
     1081 tcaataaata cgttgcttgc ctcgcggcgt tggccttagc ctgccactgg taactgttcg
     1141 agttggtctg cccgaaaggg tatggcccga gtcccggaag gtggtgagcc gctgatgaag
     1201 aacgtatcta agtcgacgat ggacgtgacg tttgctcgcg ggcttgccat gtccgaactc
     1261 ggcagcacgg gcgccacatg gcgtccggcc gtcttcatgt tcacgcccaa gttcacgccc
     1321 atgttcacgc ccatgttcac gcccaatctc gtgcccagct ccacgctcgt gcaggcagca
     1381 gttcagcacc cgtatgccgc agcaaccgca gcttttccga ccgtcaagac caccgttacg
     1441 gccgcgatca agcagcactt ggtcaagacc gacgccggtc gcgacaaccg caccactgag
     1501 ggaccctcgt ctcggaggat cccggtctga ggcaccaatc agaccggctc tccaggccgc
     1561 ggaaccctca ccgggatccg cggctttttt gttgcgcgag acccgcgacc gcaccgcacc
     1621 gcaccagctc aagctccgcc cgcagtcgca ccgccacaac tgaaggaccc gccggaatgt
     1681 cagacctcac cccgaggatc gaccccacgg caggtcgccc gcatcgcaga cgacctggct
     1741 gcgattagac ctacgacctc gtaagggggt gacgcagatg ctgaccgacc gattcgaccg
     1801 c
//
LOCUS       DASWVF010000008         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_305942, whole genome shotgun sequence.
ACCESSION   DASWVF010000008 DASWVF010000000
VERSION     DASWVF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305942"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1846
                     /locus_tag="VGD55_00115"
     CDS             29..>1846
                     /locus_tag="VGD55_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0868777.1"
                     /translation="MDGGLPVVGPTSLRLTEILKIQSSVAVPIFVDDVLWGSATIVSD
                     RAKPLPRNTEPRMAEFAALASTSIASAQRRAEVQRLADEQAALRSVATLVATGAEPEQ
                     LWERVLADVGELCQANRAQLIRYEDSMTSLSALALWSADGPHEQFAERWPVNENTMSG
                     RVAREQTVQRIEDWSSVGGGTAAAIRDRLPVSTSVMAPVFADGSLWGGIAVHSPTGEV
                     LAADTERRVVAFAELVSTAIANATARTEVRRLVDEQATIRRIATLVAQQAPLRDVCQK
                     VAEELGDLLRVEDVRLLRYGRNDDTTVLGSWGLLTERYTVGSTVVISGDVAVARVRSE
                     RTLIRLDGYDGLSGEPARLARGAGLTSTIACPIFVGDRLWGALTVASSQPGRILDRVT
                     QLLSDCLDLIATAIRNAEAHESVRASRERIVTATDQARRGFERDLHDGAQQRLVALAL
                     ELRACENLTAAALAATADEVDSILTDLRDLSRGLHPAVLSEGGLPAAVRSLARRCPTP
                     VRLALPDSWPEVNEMVETACYYVVAEALTNVAKHAQARGVDVELTLNDTGVRIVVRDD
                     GVGGASTADGSGLLGIADRVEALGGFMAVASPCEAGTTLV"
BASE COUNT          276 a          653 c          635 g          282 t
ORIGIN      
        1 cgtgtaccgg acagggcgcc cggcgcgggt ggacggcggc ttgccagtcg tcggaccgac
       61 ctccctgcgg ctgaccgaga tcctcaagat tcaatcgtcg gtcgccgtgc ccatcttcgt
      121 cgatgacgtc ctctggggct cggccacgat cgtctccgat cgggccaagc ccctgccccg
      181 taatactgag ccgcgcatgg ccgagttcgc tgccctggcc tcaacctcga tcgccagtgc
      241 gcagcgacgc gccgaggtgc agcgactggc cgatgagcag gccgccctgc gcagcgtcgc
      301 cacgctggtc gccaccggcg ccgagcccga gcagctttgg gagcgcgttc tcgccgacgt
      361 gggcgagctc tgccaagcca accgcgcgca gctgatccgc tacgaggact ccatgacgtc
      421 cctgagcgcc ctggcgctct ggtccgcgga cgggcctcat gagcagttcg ccgagcggtg
      481 gccggtaaac gagaacacca tgagcgggcg ggtcgcgcgc gaacagaccg tccagcgcat
      541 cgaggactgg tcctcggtag gaggcgggac cgccgcggcc atccgtgacc gcttgcccgt
      601 cagcacgtcc gtcatggccc cggtgttcgc cgacggaagc ctatggggcg ggatcgccgt
      661 tcactccccg accggcgagg tgcttgccgc ggacaccgaa cggcgggtgg tcgcgttcgc
      721 cgaactagtc agcacggcaa tagcgaacgc caccgcgcgg acagaggtgc gtcgcctggt
      781 tgacgagcag gccacgatcc ggcgaatcgc caccctggtc gctcagcagg caccgctgag
      841 agacgtctgc cagaaggtcg ccgaggaact cggcgacctt ctgagagtcg aggacgtgcg
      901 gctgctgcgc tacggcagga atgacgatac gacggttctt ggcagctggg ggctgttgac
      961 cgagcgttac acggtcggat cgaccgtagt catctccggc gacgtcgccg tcgcccgggt
     1021 gcgcagcgag cgtacgctga tccgccttga cggctacgac ggactctccg gtgagcctgc
     1081 ccggctggcc cgcggcgcgg gcctcacgtc aaccatcgca tgcccgattt tcgtgggtga
     1141 ccgtctctgg ggagcgctga ccgtcgcctc gtcccagccg ggacgcatcc tggatcgcgt
     1201 cacccagctc ctctcggact gcctcgacct gatcgcgacc gccatccgca acgccgaggc
     1261 gcacgagagc gtgcgcgcgt cacgcgagcg catcgtgacc gcgaccgatc aggcccgccg
     1321 gggattcgaa cgcgacctgc acgatggtgc ccagcaacgg ctggtggcac tcgccctgga
     1381 actgcgcgcc tgtgagaacc tcactgccgc ggcgctggcc gcgacggccg acgaggtcga
     1441 ctccatcctg accgatctgc gggacctgtc gcgggggctg catcccgcgg tgttgtccga
     1501 gggcggtctg ccggcggcgg tccggtcgct cgcccggcgc tgcccgacgc cggtccggct
     1561 cgcgcttccc gacagctggc ccgaggtcaa cgagatggtc gagaccgcct gctactacgt
     1621 cgtcgccgag gcgctgacca atgtcgctaa gcacgcgcag gctcggggtg tcgacgtcga
     1681 gctgaccctc aatgacaccg gcgttcggat cgtcgtccgc gacgacggtg tcggcggcgc
     1741 gagcaccgcg gacggatcag ggctgctcgg catcgccgac cgcgtcgagg cactcggcgg
     1801 tttcatggca gtggcgagcc cttgcgaagc cggcaccacc ctagtc
//
LOCUS       DASWVF010000009         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_305985, whole genome shotgun sequence.
ACCESSION   DASWVF010000009 DASWVF010000000
VERSION     DASWVF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305985"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VGD55_00120"
     CDS             <1..1333
                     /locus_tag="VGD55_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0868778.1"
                     /translation="GVAVAILQIQPLLLPLLLVSGIPLWLTGRLQSKREFKFAVGQTP
                     MLRLRNYLLQVLSDRGPAKELRAFGFGPAVRQRFDNAYANYIAALRRHVRYRSIVAFA
                     GSLVSALILGLAIGALVWMVSRGEISLTGSLTAIVGIRLMGTMITAIFSSIQQIFESG
                     LFLDDYHDFLSIKTGADTAEAGAPAPTTFGRVDVKNLSFTYPGSRQPVLRDISMHVGA
                     GEVVALVGENGSGKTTLAKMLAVLYEPDEGTISWDGVDIATFDRAGLRRAIAIIFQDF
                     VRYELTARENISAGRGGQEDPAGIAAAARQAGADRYIERLPLGYETILSKQFKGGRDL
                     SLGQWQRVALARAFYRDAPFVILDEPSSALDPRAEHDLFAHIRTLLAGRTVLFISHRF
                     STVRSADRIYVMGDGRIVEEGSHDELMAHGGTYAELFTLQAAAYLEPKTGV"
     gene            complement(1306..2289)
                     /locus_tag="VGD55_00125"
     CDS             complement(1306..2289)
                     /locus_tag="VGD55_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868779.1"
                     /translation="MTSLATYQLCGLRVASELPLHQDRIVEGGGPPDVVLTVGESRPV
                     PTAEPAGVRLAEVVDDAGTRYFSFAKNADGSYVLRSYRIGDFEVSADLSRVVLHLDPN
                     ADPGFASVLATGSLLAFLILMRGAPLLHASAVEIEGRALAFVGFSGMGKSTMATIMCA
                     GGATLITDDTLRVDLDGGEVRCFLGATETRLRTSAAELTSAFAVGGAHRHTSDDREAL
                     RLAAAHTERLPLAAIVIPQPVKQTEEVVVERLRGAEALLTLSRFPRIVGWEEPTVLQR
                     QFEFLADIVERVPVYIGYLPWGPPFAPDLAVRLLAGIAELPRTQTPVLGSR"
     gene            complement(2286..>2769)
                     /locus_tag="VGD55_00130"
     CDS             complement(2286..>2769)
                     /locus_tag="VGD55_00130"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEY0868780.1"
                     /translation="LSAPIRAGVREVVNRGIAAARELPALLAAAGVAAAVEIGLRTMK
                     LPVLARLSGTPLRDDDGTPLREADRLGLPPRLVVRMRAVRRVMRHWPFGTSCLRLALV
                     SGQRIRSVQPVLRVGVMKDADGVRAHAWLEVDGVSLDPEARFEYAVVQAASGSGRRAP
                     "
BASE COUNT          479 a          979 c          871 g          440 t
ORIGIN      
        1 cggtgtcgcc gtcgcaatcc tccagatcca gccgctgctg ctgccgctgc tactcgtctc
       61 cggtatcccg ctctggctga ccggaagact gcagagcaag cgcgagttca agttcgccgt
      121 cggtcagaca cccatgctcc gactgcgcaa ctacctccta caggtgctgt cagaccgtgg
      181 cccggcgaag gagttgcgcg cgttcggttt cggtccggcg gtccgccagc gcttcgacaa
      241 tgcctacgcg aactacatcg cggcgcttcg gcgccacgtt cgctaccgct cgatcgtcgc
      301 gttcgccggc agtttggtat cggcactgat tctcgggttg gcgatcggcg ccttggtctg
      361 gatggtcagc cgcggcgaga tctcgcttac cggctcgttg accgccatcg tcggcatcag
      421 gctcatgggc acgatgatca cggcgatctt ctcgagcatt cagcaaatct tcgaatcggg
      481 tctgttcctc gatgactacc acgacttcct gagcatcaag accggtgccg acaccgcgga
      541 agccggtgca cccgctccga cgacgttcgg gcgggtcgac gtcaagaacc tgtcgttcac
      601 ctacccgggc agccggcaac cagtgttgcg tgacatctcg atgcacgtcg gcgccggcga
      661 ggtcgtggcc cttgttggcg agaacggttc tggcaagacg acgctcgcga agatgctcgc
      721 ggtgctctac gaacctgacg aaggcaccat cagctgggac ggcgtcgaca tcgccacgtt
      781 cgatcgtgcc ggtctgcgac gcgcgatcgc gatcatcttc caagacttcg ttcggtacga
      841 actgaccgcg cgggaaaaca tcagcgcggg tcgtggcggc caggaagatc cggccggcat
      901 cgcagcggcc gcgcgacagg ccggcgccga ccgctacatc gagcggctcc cgctggggta
      961 cgagacgatc ctgtcgaagc agttcaaggg tggccgtgac ctgtcacttg gccagtggca
     1021 gcgggtcgcg ctcgcccgtg cgttctaccg cgacgcgccg ttcgtgattc tcgacgaacc
     1081 gtcgtcagcg ctcgacccgc gcgcagaaca cgatctcttc gcccacatcc gcacgttgct
     1141 cgctggccgc accgtgctgt tcatctccca tcgtttctcc accgtgcgat cagccgaccg
     1201 catctacgtc atgggtgatg ggcggatcgt cgaagagggc tcgcacgacg agttgatggc
     1261 gcacggcggc acgtacgccg aactcttcac gttgcaggcg gctgcctacc tcgagcccaa
     1321 gactggcgtc tgagttcggg gcaactctgc tatgccggcg agcaatcgga ccgcgagatc
     1381 gggggcgaac ggcggccccc acggcaggta gccgatgtag accggcaccc gctcgacgat
     1441 gtcggcgagg aactcgaact gccgctggag cacggtcggc tcctcccacc cgacgatgcg
     1501 tgggaaccgc gacaaggtca gcaaggcttc cgcgccgcgc agtcgctcga cgacgacctc
     1561 ctccgtctgc ttgaccggtt gcggaatcac gatcgccgcg agtggcagcc gttcggtgtg
     1621 cgccgccgcc agccgcaacg cctcccggtc gtcgctggtg tggcggtggg cgccacccac
     1681 cgcgaacgcg ctcgtcaact ctgcggccga cgtccggagg cgggtctcgg tcgcgccgag
     1741 gaagcagcgg acctcacctc cgtcgagatc gacccgcaac gtatcgtcgg tgatcagcgt
     1801 ggcaccgcca gcacacatga tcgtcgccat cgtcgactta cccatgccgg agaatccgac
     1861 gaacgccagc gctcgcccct cgatctcgac cgcgctcgca tgcaacagcg gcgcacctcg
     1921 catgaggatc aggaacgcga gcagcgaccc ggtcgcgagc accgaagcga accccgggtc
     1981 agcattcgga tcgaggtgca gcacgaccct cgaaaggtct gccgacacct caaagtcgcc
     2041 gatccgatag ctgcgcaaca cgtacgagcc gtcggcgttc ttggcgaagc tgaagtaccg
     2101 ggtgcccgcg tcgtcgacga cttccgctag ccgcaccccg gcaggctcgg ccgttggcac
     2161 tggccgactt tcaccgacgg tcaggacgac gtccggcggc cctccgccct caacgatccg
     2221 atcttggtgc aaaggcaact cagacgcgac acgcaagccg cacagctggt acgtggcgag
     2281 cgaggtcacg gtgcacggcg cccggatccg gaggccgcct gcacgacggc gtattcgaac
     2341 cgggcctcgg gatcaaggct cacaccgtcg acctcgagcc acgcatgcgc tcgcacgccg
     2401 tcggcgtcct tcatgacccc tacccgcaag accggctgca ccgagcggat gcgctgaccc
     2461 gacaccagag caagacgcag gcacgaggtc ccgaagggcc agtgccgcat cacccgccga
     2521 accgcacgca tgcgcacgac gagcctcggc ggcagaccga gccgatccgc ctcgcgcagc
     2581 ggcgtcccgt cgtcgtcgcg cagcggcgta ccggagagcc gcgcgaggac cggcaacttc
     2641 atcgtgcgca ggcctatttc gaccgcagcg gcaacaccgg cggccgcgag caacgcaggt
     2701 agctcgcgcg ccgctgcgat gccgcggttc acaacctcac gcacccctgc ccggatcggc
     2761 gcgctcaag
//
LOCUS       DASWVF010000010         9083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_25750, whole genome shotgun sequence.
ACCESSION   DASWVF010000010 DASWVF010000000
VERSION     DASWVF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25750"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..518)
                     /locus_tag="VGD55_00135"
     CDS             complement(36..518)
                     /locus_tag="VGD55_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0868781.1"
                     /translation="MSFNVGETVVYPHHGAALIEAIESRVINGEERIYLVLKVAQGDL
                     TVRVPADNAEFVGVRDVVGQEGLDRVFEVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRERERGLSAGEKRMLGKARQILVSELALAENTNEDKAETILDEVLAS
                     "
     gene            754..1503
                     /locus_tag="VGD55_00140"
     CDS             754..1503
                     /locus_tag="VGD55_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868782.1"
                     /translation="MDRGTGGSGGSDELPGGTPAGPPIVVPDDARELARDIAAWRREE
                     RWRRRRRMFERRFLGGPGSSRVVSVPLVITLLVAVALLGATLAFPGSTTVHPTTPPVP
                     LVLAAPSATVGTVGGLVPDDTLSGQTGTTTARSLRPALLAVAQPGCACTPALTHLAEQ
                     AASYGLTVYLIGSQAQHAQLASLASGGSPIVQVLSDQGSAITSAFSRGVLTVVGVHAD
                     GVIEVIIDDFLRTTSLDGVLAGLKQPAHAGA"
     gene            1595..2341
                     /locus_tag="VGD55_00145"
     CDS             1595..2341
                     /locus_tag="VGD55_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868783.1"
                     /translation="MSRSKVHRWRRPGLRAAVLLVAGLPTLLACSATGFGSPTRHAIA
                     NLQAATTKIGTTLQIEDAIIALPSDDLSLKGGLAYLEMDAINFANQPDELMSVTADVD
                     MAGSATPSPAAGSASSPASTSSPTQPAAQSLQAASITTVPAATAGGPGTLRITVLLRT
                     LTQPLRQGDTVTIGLTFHNSGTVSGLLVPVQGAEVVGTFLPTAPPPAPSSASATPTPA
                     SSAPASVPASAPASAPASTSPASPSAAATS"
     gene            complement(2351..3100)
                     /locus_tag="VGD55_00150"
     CDS             complement(2351..3100)
                     /locus_tag="VGD55_00150"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEY0868784.1"
                     /translation="MSDHTLVLLRHGESQWNAKNLFTGWVDVDLSAKGEAEAANGGRL
                     LAENAVMPDVVHTSVLRRAIRTANIALEAADLLWLPVQRSWRLNERHYGALQGKDKKA
                     TLEAFGEEQFMLWRRSYDVPPPAIAADDEFSQTADPRYATLPPELRPTTECLKDVVHR
                     MLPYWYDAIVPDLLVGKTVLVVAHGNSLRALVKHLDQISDDDIAALNIPTGIPLVYRL
                     DADFVPTVRGGEYLDPDAAATAAAAVANQGR"
     gene            complement(3144..3677)
                     /locus_tag="VGD55_00155"
     CDS             complement(3144..3677)
                     /locus_tag="VGD55_00155"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0868785.1"
                     /translation="MSWLARTEIDGVIRATLEEREISFDEVGESAFVVRLVGTRRLVT
                     AVRLVVGDQSLLVEAFFVRRPDENAAEFYRFLLERNGRMYGVHFAIDSKGDAYLIGRL
                     PLLAVTPDEIDRLLGCVLTYADETFDAALEIGFAESIRREWDWRTSRNEPVENLRAFA
                     RFADPEAPGGGRHRRGA"
     gene            complement(3703..4986)
                     /gene="mshA"
                     /locus_tag="VGD55_00160"
     CDS             complement(3703..4986)
                     /gene="mshA"
                     /locus_tag="VGD55_00160"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEY0868786.1"
                     /translation="MPVHRVAMLSLHTSPVEQPGVGDAGGMNVYVMELSRRLAARGVE
                     VEVFTRATSGDVPAVQRIEPGFIVRNVIAGPLEPLPKADLPSQLCAFTAAVLRAEATR
                     EPGWYDLIHSHYWLSGQVGWLAKERWGVPLVHSMHTLARVKNAALAVGDAPEPSARVI
                     GEGQVVDAADRLIANTEAEARQLIELYDADPMAVSVVPPGVDLSLYRPGSRAAARARL
                     SLPADREILLFVGRIQPLKAPDLLIRALAVMVDRTPTLRDRLMAVVVGGVSGAGAVEP
                     GQLRRLAERLGVDDLVRFEPPASGDRLADFYRAASVTVVPSYSESFGLVALESQACGT
                     PVVAARVGGLVTTVSEGRSGLLVDGHEPQCYADALSELLADAELRRSLSTGAVAHAAG
                     FDWDRTVDGVIDVYADALSGAAARSVEGLPVAAVR"
     gene            5295..6050
                     /locus_tag="VGD55_00165"
     CDS             5295..6050
                     /locus_tag="VGD55_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0868787.1"
                     /translation="MSSDPTSGRRIAVVTGASSGIGAATARHLAREGFDVVCAARRAD
                     RLAEVAEEIGGRAIVVDVTEADSVAALAQAVPTCSLLVNVAGGAVGAEPISTADPADW
                     QTMFDVNVLGVLRVTQALLPSLIASGAGHVIVVGSTAGRIVYEGGGGYTAAKHGVAAL
                     AETLRLELSGQPVRVTEVAPGMVRTDEFSLNRFRGDADRAAAIYAGVAEPLTADDIAD
                     CISWCATRPPHVNVDLLVVKPLAQAAQYKVHRT"
     gene            6047..6505
                     /locus_tag="VGD55_00170"
     CDS             6047..6505
                     /locus_tag="VGD55_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868788.1"
                     /translation="MTEPLDITDRADHQHVVNNGKRDLTLEQLATTQPGLDRLMAELG
                     PRMHRLYFAGRAENWRLADYFFRSVVKQLSLCAFSRPKYGEAINTYLATDCEPVRVAI
                     KNGDRDAFETAYTAMVARANGYHDEYAKPYLHWVCPAAAPEDIDFTAGLS"
     gene            complement(6522..6809)
                     /locus_tag="VGD55_00175"
     CDS             complement(6522..6809)
                     /locus_tag="VGD55_00175"
                     /inference="COORDINATES: protein motif:HMM:NF022184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEY0868789.1"
                     /translation="MHLASSATNVILLVALAIEVVALVDALTHPAGAYLAAGKLTKQA
                     WVAILAVAGVVVLLSGWFGPLGLAAIIATIVYFVDVRPALRRVRGPRTSRR"
     gene            complement(6858..7820)
                     /locus_tag="VGD55_00180"
     CDS             complement(6858..7820)
                     /locus_tag="VGD55_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0868790.1"
                     /translation="MTDMTYRQLGNSGLTVSTVGLGCNQIGRKLDLDGTRAVVDAAIE
                     GGLTLFDTADIYGNEVGASEALLGQALQGRRDRVVLATKFGMSTRGVTLPDYEARGSR
                     RYVRASIEASLRRLRTDHVDLYQMHEPDPRTPIEETLEALDELVREGKVRYIGSSNFT
                     GWQLVDADWAARANGGARFVSAQNEYSLLERDAEIELIPAAEHVGVGVIPYFPLARGL
                     LTGKYRRGEPAPVGTRLADRTEFLDTADWDVVENLQKFADARDLTLLEVAIGGLAAQP
                     GVASVIVGATRPEQVHANAKAGQWEPAAVDLIELDDITGSVRGR"
     gene            complement(7971..8447)
                     /locus_tag="VGD55_00185"
     CDS             complement(7971..8447)
                     /locus_tag="VGD55_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868791.1"
                     /translation="MSRADDFLSSIQKAVGDTPVLLLAGAGDLVSEKLRELPDAVSAW
                     QAENRDFPMRAAGALIGSAFRANLRVGELYDELTRRGEDVLTKMRGDEVVAEEDEPFL
                     REPYMPEPVRPPSRVAKKHAVTKKSAAKKSAAKSTTMTSSTKSAAKKTATKKSSAK"
     gene            complement(8444..>9083)
                     /locus_tag="VGD55_00190"
     CDS             complement(8444..>9083)
                     /locus_tag="VGD55_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0868792.1"
                     /translation="LTGEAVFRRVRASGALAIASSRGETVQMAQNFDLGEFIREQRRT
                     AQISVRQLAKLAGVSNPYLSQIERGLRRPSAEIMQQIAKGLRISAEALYVQAGILDER
                     VVGGVVIDAIIADPTITERQKQVMVEVYDSFRKENLRRDADEAALAQASAGPQAPDSA
                     HASVESVVEQVSSGAGSKSPGSHRGTTPSSHSAKVTPLRTTMSRSTSPRKPR"
BASE COUNT         1563 a         3133 c         2881 g         1506 t
ORIGIN      
        1 atcgcggcgc accggacgga accggtgggc acgggtcagg aggccaggac ctcgtcgagg
       61 atggtctcgg ccttgtcctc gttggtgttc tcggccaggg ccagctcgct gaccaggatc
      121 tgcctggcct tgccgagcat gcgcttctcg ccggcggaca ggccgcgctc gcgctcgcgg
      181 cgccacaggt cgcgcacgac ctcggccacc ttgttgacgt cgccggacgc cagcttctcg
      241 aggttggcct tgtaccggcg cgaccagttg gtgggctctt cggtgtgcgg tgcgcgcagc
      301 acctcaaaaa cccggtcgag cccctcttga cctacgacat cgcggacgcc gacgaactcg
      361 gcattgtcgg caggcacccg gacggtgaga tcaccctggg caaccttcaa gacgaggtaa
      421 atccgctcct cgccattgat gacacgactc tcgatggcct cgatcagtgc tgccccgtga
      481 tgggggtaga cgaccgtctc gccgacgttg aacgacatgt ggcacaggcc ccttccgctg
      541 gttcaacgat accacgcaaa acggcgccgc gggaacgtcc gcagacacat ctgcgcaggt
      601 cagagccatg gtgacagcaa acgcaagatc accagcggtc gattgaggtc ttgacaaacg
      661 gcgggtggtt gtgccccaca accccctttt cttcatccac cggaaccctc cccggcctgg
      721 gcgcggcacc cggtccgtgc ccatacttgg cagatggacc ggggcactgg gggcagcggg
      781 ggcagtgacg aactgcccgg cggcactccg gctgggccgc cgatcgtcgt ccccgatgac
      841 gctcgtgaac tcgctcgcga catcgccgcg tggcgacgag aggagcgctg gcggcgccgc
      901 cgacgcatgt tcgagcggcg attcctgggc gggccgggct cgtcgcgggt cgtctcggtc
      961 ccactggtca tcaccctgct ggtagcggtg gccctcctcg gcgcgacgct ggccttcccc
     1021 gggagcacga cggtgcaccc gacgacaccg ccggtgccgt tggtgctcgc ggcgccctcg
     1081 gcgacggtcg gtaccgtcgg cggactcgtc cccgacgaca cccttagtgg acagaccggc
     1141 actaccaccg cgcgttccct gcggccggcg ttgctcgccg ttgcgcagcc gggctgtgca
     1201 tgcaccccgg cgctgacaca tctggccgag caagccgcga gctacggcct cacggtctac
     1261 ctcatcggaa gccaggcaca gcacgcccaa ctcgcatccc tcgcgagcgg cggcagcccc
     1321 atcgtgcagg tcctgagcga tcaaggttcg gcgatcacct cggcgttttc acgcggagtc
     1381 ctgacggtcg tgggggttca cgcggacggc gtgatcgagg tgatcatcga cgacttcctg
     1441 cgcaccacgt cgcttgatgg cgtgctcgcc ggactcaagc agcccgcaca tgctggggcg
     1501 tgatcaccgc ctgaaagcgg ctcgttaggc tacggccctg aacggacacg gccgacggcc
     1561 ttgagggcca gtcggcagcc gaaagggaca ggtcgtgagc aggagcaagg tccaccggtg
     1621 gcgccgcccg gggctgcgag ccgcagtcct gctggtcgcg ggccttccaa cactgctcgc
     1681 ttgctcggcc accgggttcg gatcgccgac gcggcacgct atcgccaacc ttcaggcagc
     1741 gaccacgaag atcgggacga cgctccagat cgaggacgcg atcatcgcgc tgccaagcga
     1801 cgacctgtcc ctcaagggcg ggctcgccta cctggaaatg gacgcgatca acttcgcgaa
     1861 ccagcccgac gagctgatga gcgtgaccgc tgacgtcgat atggccggca gtgcgacccc
     1921 aagccctgcg gcaggctcgg cttcgagtcc cgcatcgacg tcgagtccta cccagccagc
     1981 cgcacagtcg ctccaggccg cgagcatcac gaccgtcccg gccgcgacgg ccggcgggcc
     2041 gggcacgctt cgcatcaccg tgctgctgcg cactctcacg cagcccctgc gtcagggcga
     2101 caccgtcacg atcggcttga cgttccacaa cagcgggacg gtcagcgggt tgcttgtccc
     2161 cgtccagggc gcagaggtcg tcggcacgtt cctcccgacc gccccgccgc cggctccctc
     2221 gtcggcgagt gcgacaccga ccccggcttc atccgcaccc gcgtctgtac cagcgtcggc
     2281 acctgcgtcg gcacctgcgt cgacctcgcc ggcgtcaccg tcagccgccg ccacgagcta
     2341 acgcgcagta tcagcggccc tgatttgcca cggcggccgc agcagtagct gcagcatcag
     2401 ggtcgaggta ttcgccgccg cgcaccgtcg gaacgaagtc ggcgtcaagg cggtagacga
     2461 gcggaatgcc agtagggatg ttcagcgccg cgatgtcgtc atctgagatc tgatcgaggt
     2521 gctttaccaa cgcacgcagt gaattcccgt gtgcgacaac gagaaccgtc ttgcccacga
     2581 gcagatcagg cacgatcgcg tcgtaccagt acggcagcat gcggtgcacg acgtccttca
     2641 aacattcggt cgtcggacga agctcgggcg gcagcgtcgc gtagcgcgga tcagcggtct
     2701 gcgagaactc gtcgtcggca gcgatcgccg gcggcggcac gtcgtacgac cggcgccaca
     2761 gcatgaactg ctcttcgccg aacgcctcga gtgttgcctt cttgtctttt ccttgaagtg
     2821 cgccgtagtg acgttcgttc aaacgccatg agcgctgcac cggcaaccac agcaggtctg
     2881 ccgcttcgag cgcgatattc gcggtgcgaa tcgcacgccg caacacagag gtatgtacga
     2941 cgtctggcat gaccgcgttc tcggcgagca ggcgcccgcc gttcgcggct tcagcttcac
     3001 ccttggcgga gaggtcgacg tcgacccagc cggtaaagag attcttcgcg ttccactgac
     3061 tttcgccgtg acgcagcagt acgagcgtgt gatccgacat gggactaagt ttcccagacc
     3121 gcgcagggcg gctcgcatac gggtcaagcg cctcgacgat gccgcccgcc accgggagct
     3181 tcaggatcag cgaatcgcgc gaacgctcgc aagttctcga ccggttcgtt gcgcgacgtc
     3241 cgccaatccc actcgcgacg gatgctttct gcgaagccga tctcgagcgc cgcatcgaac
     3301 gtttcgtctg cgtatgtcag cacgcaaccg agcagccgat cgatctcgtc aggtgtgacg
     3361 gccagcaatg gcaagcggcc gatcaggtag gcgtcgccct tgctgtcgat cgcgaaatgc
     3421 acgccgtaca tgcggccgtt gcgttcgagc aggaaccggt agaactccgc cgcgttctcg
     3481 tctggacgcc gcacgaagaa tgcctcgaca agcaagctct gatcgccgac gacgagacgc
     3541 accgccgtaa cgagccgtcg cgtcccgaca aggcggacga cgaaagccga ctcgcccacc
     3601 tcgtcgaagc tgatttcgcg ctcttcgagg gtcgcccgga tgacaccatc gatctcggtg
     3661 cgcgcaagcc aactcatggg gcgcccgccc cggtcagtgc cattaccgca ctgcggctac
     3721 gggcagccct tcgacacttc gggccgccgc accgctcaac gcatcggcgt agacatcaat
     3781 cacgccatcg actgtacggt cccaatcgaa ccccgcggcg tgggcgacgg cacctgttga
     3841 caacgagcgg cgcaattcgg cgtccgcgag aagctcggaa agagcgtctg cgtagcactg
     3901 cggctcgtgt ccgtcgacga gcaacccgga gcgcccttcg ctcaccgtcg tcaccagacc
     3961 acccactcgc gccgcgacga caggagtgcc gcaggcctgt gattcaagcg cgacaagccc
     4021 gaacgattcg ctatacgacg ggacgacggt cacgctagcc gctcggtaga agtcggcgag
     4081 acgatcacct gacgccggcg gctcgaagcg caccagatcg tcaaccccca ggcgctcggc
     4141 gaggcggcgc agctgccccg gctcgacggc tccggcgccg ctgactccgc ctacgacgac
     4201 tgccatgaga cggtcacgca gcgtcggcgt gcgatccacc atcacggcga gtgcacggat
     4261 cagcaggtcg ggcgccttca gcggttggat ccgcccgacg aacagcagaa tctcgcgatc
     4321 cgctggcagc gacagacggg cccgcgccgc cgcacgggat ccggggcggt acagcgacag
     4381 atcaacacca ggtggaacga cggagaccgc catcgggtcg gcgtcgtaga gctcgatcaa
     4441 ctgccgcgcc tcggcctcgg tattggcgat caaccggtcg gcagcgtcga cgacctggcc
     4501 ctcaccgatg accctggccg acggttcagg cgcgtcgccc acagcgagcg cggcgttctt
     4561 gacccgggcg agcgtgtgca tgctgtgcac gagcggcacg ccccagcgct ccttcgcgag
     4621 ccaaccaacc tggcccgaca gccagtagtg ggagtggatc aggtcatacc aaccaggctc
     4681 ccgcgtggct tcggcgcgca gcaccgcggc ggtgaaggca cacagctgcg acggcagatc
     4741 tgccttcggc agcggctcga gcggtccggc gatcacgttg cggacgatga aaccaggctc
     4801 gatgcgttgg acggcgggaa cgtcgcccga cgtcgcacgg gtgaagacct cgacctcgac
     4861 acctcgggcg gcaagtcggc gcgacaactc catgacgtag acgttcatgc cgcccgcgtc
     4921 acctacaccc ggctgctcga cgggggaggt gtgcaagctc agcatcgcca cccgatgaac
     4981 cggcatcgcc cggcgccttg agcggcgccg caggctagag accggcgccg gtgttgacga
     5041 tgcggtcggg ggaagcatcg gggcaagcgc aacatcgctc gacacttact tcctcctcat
     5101 cgtgtgtcac cggctcatcg cgccaccggc actgaacacg ctagacgagc gtctcgccgg
     5161 gccacccact ccacccagag gcgcaacgcc tgagcccggc cgaccattcc cgcgatgacg
     5221 cctccggccg gtgaccctct tgcgagcgtt gcccgcctcg gcaggagcca aaccggcact
     5281 gccaagatgc cctcatgagc agtgacccaa cctcgggccg ccggattgcg gttgtgaccg
     5341 gggcgagcag tggcatcggc gcggccacgg ctcgccacct cgcgcgcgag ggcttcgatg
     5401 tcgtctgcgc cgcccggcgt gcggatcgcc tcgctgaggt ggccgaggag atcggcgggc
     5461 gcgccatcgt cgtcgacgtg accgaggccg attcggtcgc tgcgctcgcc caagcggtgc
     5521 cgacgtgttc gttgctcgtc aacgtggcgg gcggcgctgt cggggccgag ccgatctcga
     5581 ccgccgatcc ggccgactgg cagacgatgt tcgacgtgaa cgtgctcggc gtgctgcgag
     5641 tcactcaggc gctgctgccg tcgctgattg cgtcaggcgc gggccacgtc atcgtggtcg
     5701 gttcgaccgc cggccgcatc gtttacgagg gtggcggcgg ctacaccgcc gcgaaacacg
     5761 gagtcgcggc gctcgccgaa accttgcgtc tcgagttgag tggccagccg gtgcgtgtca
     5821 ccgaagtcgc gcccgggatg gtgcgtacgg acgagttcag cctgaaccgc tttcgagggg
     5881 acgccgaccg tgcggccgcc atctacgccg gcgttgccga accgctgacc gcagacgaca
     5941 tcgctgactg catcagttgg tgtgcaactc gaccaccgca cgtgaatgtc gatctactcg
     6001 tcgtgaaacc gctcgcgcaa gccgcccagt acaaggtgca caggacatga ccgagccact
     6061 cgatatcaca gacagagccg accaccaaca cgtcgtcaac aacggcaagc gcgacctcac
     6121 gctcgagcag ctcgctacca cacaacctgg acttgaccgc ctgatggcgg agctcggtcc
     6181 gcgcatgcat cggctgtatt tcgcaggcag agctgagaat tggcggctcg ccgactactt
     6241 ctttcggtcg gtcgtgaagc agctgagcct ctgcgcgttc tcacgaccga agtatggcga
     6301 agccatcaac acctacctcg cgaccgactg tgaacctgtg cgcgttgcta tcaagaatgg
     6361 cgatcgcgac gcattcgaaa cggcgtacac cgcgatggtt gcgcgagcga acgggtacca
     6421 cgacgaatac gcgaagccct acctgcattg ggtttgtccc gcggcagcac cagaagacat
     6481 cgacttcaca gcggggctga gctagtcgtc gtccgccttg gtcatcgtct ggaagttcgc
     6541 ggcccgcgca ctcgtctcaa tgccggacgg acgtcgacga agtagacgat ggtggcgatg
     6601 atcgctgcga gcccgagcgg accgaaccac ccactcagca agacgacgac gccggcgacg
     6661 gccaggatcg cgacccacgc ctgcttggtc aatttccctg ccgcgaggta agcaccggcc
     6721 ggatgcgtca gtgcgtcgac caacgcgacg acctcgatcg ccagcgcgac aagcaggatc
     6781 acgttggttg ccgacgatgc gaggtgcatg ccctccacgt tagcggcagt tcggcgccgc
     6841 ggcggccgga tcgaggttca gcgaccccgc acgctgcccg tgatgtcgtc gagttcgatc
     6901 agatcaaccg cagccggctc ccattgaccg gccttcgcgt tcgcgtgcac ctgctcgggc
     6961 cgggtggcgc cgacgatcac gcttgccacg cccggctgcg ctgcgagccc gccgatcgcg
     7021 acctcgagca aggtgaggtc acgcgcgtcg gcgaacttct ggagattctc gacgacgtcc
     7081 cagtccgcgg tgtcgaggaa ttctgtgcgg tcggcaagac gggtgccaac aggtgcgggt
     7141 tcgcccctcc gatacttgcc ggtgagcagc ccgcgcgcga gcgggaaata cggaatcacg
     7201 ccgactccta cgtgttcggc cgccgggatg agttcgatct cagcgtcgcg ctcgagcagg
     7261 ctgtattcgt tctgcgcgct gacgaaacgc gcgccgccat tcgcccgagc tgcccaatcg
     7321 gcgtcgacca actgccagcc ggtgaagttc gacgacccga tgtagcgaac cttgccttca
     7381 cgcacgagtt cgtcgagcgc ttcgagtgtc tcctcgatcg gcgtccgcgg atcgggctcg
     7441 tgcatctggt agaggtcgac gtgatcggtg cgtagccgtc gcagtgacgc ttcgatcgac
     7501 gcgcgcacgt agcgacgcga accgcgcgcc tcgtagtcgg gcaacgtcac accacgcgtc
     7561 gacatgccga acttggttgc gaggactacg cgatcgcgtc gaccctgaag tgcctggccc
     7621 agcaacgcct ctgacgcacc gacctcgttg ccgtagatat ctgcggtgtc gaacagagtg
     7681 agtccgccct cgatcgccgc gtcgaccaca gcccgcgtgc catcgaggtc gagcttgcgc
     7741 ccgatctgat tgcagccaag accaaccgtc gaaacagtca ggccggaatt cccaagctgg
     7801 cggtacgtca tgtcggtcat gtcgtcgagc ctacgaaacg tcagacgtca actcggcacg
     7861 tacccgtcgg ctgaccgtct tcggcgcgga acagtgcgcg ccaccgagtc gtcggctcac
     7921 gtccgcagtc gggtgatctt gtgtcggctg tgtcgaacgg tgctcctgca ttacttcgcg
     7981 gaagacttct tcgttgcggt cttcttcgcc gcgctttttg ttgagctcgt catcgttgtg
     8041 ctcttggcgg cggacttctt cgcggcggac ttcttcgtca ccgcatgctt cttcgcaacg
     8101 cgcgagggag gccgtacagg ttcgggcatg tacggctcgc gcaagaacgg ctcgtcttct
     8161 tctgcgacta cctcatcgcc gcgcattttt gtcagcacgt cttcgccgcg tcgcgtcagt
     8221 tcgtcataga gctcaccgac tcgaaggttc gcgcgaaatg ccgagccgat gagcgccccg
     8281 gccgcacgca tcgggaagtc gcggttttcc gcctgccacg cactcaccgc gtcgggcaac
     8341 tcgcgcaact tctccgacac gagatcgcct gcacccgcaa gcaagagcac cggcgtatcg
     8401 cccaccgcct tctggatgct gctcaagaaa tcgtcggccc ggctcatcgc ggcttcctcg
     8461 gcgacgtgga acgcgacatc gtggtgcgca ggggcgtgac cttcgcagag tgggacgacg
     8521 gggtggtgcc ccgatgcgat cccggcgact tcgacccggc gccgctagat acctgctcga
     8581 ccaccgactc caccgacgcg tgcgcgctgt caggtgcctg cggtccagca gatgcctgag
     8641 caagtgcggc ctcatcggcg tcgcgcctga gattttcctt gcggaacgag tcgtacacct
     8701 cgaccatcac ctgcttttgt cgttccgtga tggtcggatc ggcgatgatc gcgtcgatca
     8761 cgacgccgcc gacaactcgc tcgtcgagaa tgcccgcctg cacgtacaac gcctcggccg
     8821 agatgcgcag acccttggca atctgctgca tgatctcggc gctcggccgg cgcagtccgc
     8881 gctcgatctg gctgagatag gggttggaaa cgcccgccag cttcgccagc tgccggaccg
     8941 agatctgcgc cgtgcgccgc tgctcgcgga tgaactcgcc aaggtcgaag ttttgtgcca
     9001 tctgcacagt ttcccctcga ctgctagcta ttgcaagcgc gccgctagca cgcacccgcc
     9061 gaaagaccgc ctcgccggtt agc
//
LOCUS       DASWVF010000011         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_25774, whole genome shotgun sequence.
ACCESSION   DASWVF010000011 DASWVF010000000
VERSION     DASWVF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25774"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGD55_00195"
     CDS             <1..484
                     /locus_tag="VGD55_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0868793.1"
                     /translation="VLELLYATGIRVGELVGLDVDDLDDARHVVRVLGKGNKERTVPL
                     GIPALKAVERWRRSGRPQLATSASGSALFLGARGSRLDQRAVRRMVHARLASVPGAPD
                     LGPHGLRHSAATHLLEGGADLRTVQEILGHATLATTQIYTHVSVERLRATYDRAHPRA
                     "
     gene            459..1307
                     /gene="whiG"
                     /locus_tag="VGD55_00200"
     CDS             459..1307
                     /gene="whiG"
                     /locus_tag="VGD55_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEY0868794.1"
                     /translation="MTARTPEPESSGVLAATPEDDPATAKLNAEAALRDLWREFKESG
                     ESRLRERLILHYSPLVKYVAGRVGVGLPPNIEQADLVSYGIFGLIDAIEKFDLERAIK
                     FETYAISRIKGAIIDELRAIDWIPRSVRYKAREVEKAYAALEAKLHRTPTESEVAGEL
                     GITLDELHTIFSQVSFVNVIALDELLNVGGERGDKLSLVDTLEDTRAEDPVEVFETEE
                     TKYLLSRAINTLPEREKIVVTLYYYEGLTLAEIGQVLGVTESRICQMHTKAVLQLRAK
                     LAEQSA"
     gene            complement(1287..1826)
                     /locus_tag="VGD55_00205"
     CDS             complement(1287..1826)
                     /locus_tag="VGD55_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0868795.1"
                     /translation="MATPRWSWLAGLLTTTFMLGVGAPSRAAAGSGRGFAAPLAGPLE
                     VTRRFAPPDRPWEPGNRGVDLATNAGAAVYAATAGVVLYAGELAGRGVISIRDGTLRT
                     TYEPVDPVVHAGDVVTEGEVIGYVAEALDNCGSPGSCLHWGALRSNAYVDPMSLLRAP
                     AIRLLPIWNGPVELSRLLG"
BASE COUNT          385 a          711 c          672 g          333 t
ORIGIN      
        1 ggtgctcgag ttgctgtacg cgaccggcat cagagtgggg gagttggtcg gcctggacgt
       61 cgatgacctc gacgatgcac ggcacgtcgt gcgagtgctc gggaagggca acaaggagcg
      121 aaccgtgcca ctgggcatcc cggcacttaa ggccgtcgag cgctggcgcc ggtccgggcg
      181 gccccagctg gcgacgtccg ccagtggatc tgcattgttc ttgggtgctc gtggcagtcg
      241 cctcgaccag cgcgcggtcc gccgaatggt ccacgctcgg ctcgcctcgg tgccaggcgc
      301 accggacctt ggtccccacg gcctgcggca cagcgcggca acccacctac ttgagggtgg
      361 cgctgaccta agaaccgtcc aggagatact tggtcacgct acgctcgcaa cgacacagat
      421 ctatacacac gtttccgttg agcgactaag ggcgacctat gaccgcgcgc accccagagc
      481 ctgaaagctc cggcgtgcta gcggcaacac ctgaagacga tccggcgacc gcgaagctca
      541 acgccgaggc cgcgttgcgc gacctctggc gcgagttcaa agaatcgggc gaaagccgcc
      601 tccgcgagcg gctgatcttg cactactcgc cgctggtcaa gtacgtcgcc ggccgggtcg
      661 gggtgggctt gcccccgaac atcgaacagg ccgacctggt ctcctacggc atcttcggcc
      721 tgatcgacgc catcgagaag ttcgatctcg agcgggcgat caagttcgag acgtacgcga
      781 tcagccgcat taagggcgcg atcatcgacg agcttcgcgc gatcgactgg atcccgcggt
      841 cggttcgcta caaggcgcgt gaggtcgaaa aggcgtacgc cgcgctcgaa gccaagctgc
      901 atcgcacccc gaccgaatcc gaagtcgcgg gcgagctcgg catcaccctt gacgaactgc
      961 acacgatctt cagccaggtc tcgttcgtca acgtgatcgc gctcgatgag ttgctcaacg
     1021 tgggcggcga gcggggcgac aagctctcac tcgtcgacac cctcgaagac acccgggccg
     1081 aagaccccgt cgaggtattc gagactgaag agaccaagta cctcctgtcg cgcgcgatca
     1141 acacgttgcc cgaacgcgag aagatcgtcg tgacgttgta ctactacgag ggcctcacgc
     1201 tcgccgagat cggtcaggtg ctcggtgtga ctgagagtcg catctgtcag atgcacacca
     1261 aggcggttct ccagctacgg gccaaactag ccgagcagtc ggcttagctc gaccggcccg
     1321 ttccagatcg gcagtagtcg gattgcgggc gccctcaaga gactcatcgg gtcgacgtac
     1381 gcgttcgagc gcaacgcgcc ccaatgcagg cagctacccg gcgagccgca gttgtcgagc
     1441 gcctcggcga catacccgat cacctcaccc tcggtgacaa catcgcccgc gtgcacgaca
     1501 gggtcgacgg gctcatacgt cgttcgcaag gtgccgtccc ggatcgagat caccccgcgc
     1561 ccggccagct cgcccgcata aaggacgacg cccgcagtgg ccgcgtagac agccgcaccg
     1621 gcgttggtgg cgaggtcgac ccctcggtta cccggttccc acggccggtc aggcggcgcg
     1681 aacctgcgcg tcacctccag cgggcctgcg agcggcgcag caaacccgcg gccagagccc
     1741 gccgccgctc ttgagggagc accgacaccc agcatgaagg tggtcgtcag cagcccagca
     1801 agccaggacc atcgaggggt agccattcct ggagcttggg gggtccaggc agcgcaggac
     1861 acctcgtcac cggcttcggt gtacgacgcg agcgctcggc gggctgctgt ggacaactcc
     1921 cgctgacggc cactgacggc ggtgattggt cacgctcgga gacgctcgcg tagactcgcc
     1981 aacgaggtcg ggttcgcccg gcctacatcg cacgccctac caccgccccg gctcctttac
     2041 aggtgctcag ggtcggctgc cactcggtgc cttcggccct aggtccccta gatcgaggtg
     2101 t
//
LOCUS       DASWVF010000012         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_229679, whole genome shotgun sequence.
ACCESSION   DASWVF010000012 DASWVF010000000
VERSION     DASWVF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229679"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="VGD55_00210"
     CDS             complement(<1..2057)
                     /locus_tag="VGD55_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0868796.1"
                     /translation="MARSETTHVAPNESGQEETVLDVYGATLTRSRFVKGAGALVVGA
                     SLVGSGIVAKAAQADSITHSLDPTQPASWFTIHPDNTITMRTGIAEMGQGSASTAFAQ
                     FAAEQLNVSYSQITKVIRASTDETPDGGLAAGMMFLGGPNIMKAAAYTYQALLALASQ
                     KLGVPVTGLSVTNGVVSGGGKTISYGQLVQDQALNLSIPVTGEVTSFIGLSVLGNPPM
                     KPVSEYQVIGQSIPMRTIPPIVAGTATYVGDVNIAGMLHGRPVHPKNLGSQLVAVGTL
                     KKQDFPDTQIVVKGNFVGVVDPNEYTAIEAAALLAGTTKWSDWEGLPDQGDLFSAMRE
                     LDWKTTPINYGVNQGNPTAAFASATTKLANTYEFPFSKHAAIGPTCAVADCRSDGTVW
                     VYAHGQNISAGRGQFANMLGTSVDNVIVRWFDGSGHYGRSNGGNTGAEEEAIILSKAV
                     GKPVRVQWMRWDDMQWSTQHPPVISDVEAGLDAKGNLVSFSASHYEPAGQDDRLVGAL
                     LAGLPAGYAPEVFNPLNPYSSTQNSISDTWVYDQVPNAAQLAYGTTNIGTNMNDPGYQ
                     TEIGMRGHSMRTPGQRQQNFAHESMMSELAASIKMDPFEFRLKNTSAERLINVINEVI
                     KASGWETRPSPNAKAATTGSTPLTGQGCSVMLRSNAYWACVAHVSVTPSTGKVSVLDL
                     TTVV"
     gene            complement(2059..2568)
                     /locus_tag="VGD55_00215"
     CDS             complement(2059..2568)
                     /locus_tag="VGD55_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0868797.1"
                     /translation="MASTLKIKVNGLTHTVSAGLDTPLLYILRNELHLKGPRFGCGLA
                     QCGACSVLLNGKEIRSCVTPVAAVTGGQITTLEGLPAVWAAKRGTAATVDVLHPLQQA
                     WIDVQVPQCGYCQSGMIIQAADLLGTTKNPTREQIQVAMNVHLCRCGTYTAIIEAIQM
                     AAATMAKGA"
     gene            2788..3285
                     /locus_tag="VGD55_00220"
     CDS             2788..3285
                     /locus_tag="VGD55_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868798.1"
                     /translation="MMKQRGRALGRSVVVRGSVALGGLCTLAALVAVAPAGAALRAHG
                     TAALVTVTVSDSAMRVSATSASSGPTTFVLVNHGKKLHALVISGPGVKKAQTQKVTAG
                     HSAEITVTLRPGAYELSDPVGLGAYDVQFLDAIPATTVSATGSSAVVTPPAPLPPMCG
                     QYYSP"
BASE COUNT          486 a         1139 c         1206 g          597 t
ORIGIN      
        1 acgaccgtcg tcaggtcgag cacgctcacc ttgccggtgc tcggcgtcac gctcacgtgc
       61 gcgacgcagg cccagtaggc gttcgagcgc agcatgacgc tgcagccctg gcccgtgagc
      121 ggggtcgagc cggtcgtggc agccttggcg ttcggcgagg ggcgggtctc ccagccggag
      181 gccttgatca cctcgttgat cacgttgatc agccgctccg cgctcgtgtt cttgagccgg
      241 aactcgaacg gatccatctt gatcgacgcg gcgagctcgc tcatcatcga ctcgtgcgcg
      301 aagttctgct ggcgctgacc gggcgtgcgc atgctgtgcc cgcgcatgcc gatctcggtc
      361 tggtagccag ggtcgttcat gttcgtgccg atgttcgtcg tcccgtaggc gagctgggcg
      421 gcgttgggca cctggtcgta gacccaggtg tcggagatgc tgttctgggt cgaggagtac
      481 gggttgagcg ggttgaacac ctccggcgcg tagccggccg ggaggccggc aaggagcgca
      541 ccgacgagac ggtcgtcctg gccggcgggc tcgtagtggc tggccgagaa cgagaccagg
      601 ttgcccttgg cgtcgaggcc cgcctcgacg tcggagatca ccggcggatg ctgggtcgac
      661 cactgcatgt cgtcccagcg catccactgc acccgcaccg gcttgccgac ggccttggag
      721 aggatgatcg cctcttcctc ggcgccggtg ttgccgccgt tcgagcgccc gtagtggccg
      781 gagccgtcga accagcgcac gatcacgttg tcgacggagg tgccgagcat gttcgcgaac
      841 tgcccgcgcc cggccgagat gttctggccg tgggcgtaca cccagacggt gccgtcggag
      901 cggcagtcgg cgaccgcgca ggtcggaccg atcgccgcgt gcttggagaa cgggaactcg
      961 taggtgttcg cgagcttggt cgtcgccgag gcgaacgctg cggtgggatt gccctgattg
     1021 acgccgtagt tgatcggggt cgtcttccag tcgagctcac gcatcgccga gaagaggtcg
     1081 ccctggtcgg gcaggccctc ccagtcgctc cacttggtcg tgcccgcgag cagtgccgcg
     1141 gcctcgatcg ccgtgtactc gttcgggtcg acgacgccga cgaagttgcc cttgaccacg
     1201 atctgggtgt cggggaagtc ctgctttttg agcgtgccga ccgcgacgag ctgtgagccg
     1261 aggttcttcg gatgcaccgg gcggccgtgc agcatgcccg cgatgttcac gtcgccgaca
     1321 taggtcgccg tgccggcgac gatgggcggg atcgtgcgca tcgggatcga ctgcccgatc
     1381 acctggtact cgctgaccgg cttcatcggc gggttgccga gcacgctcag cccgatgaag
     1441 ctcgtgacct cgccggtgac cgggatcgag aggttgagcg cctggtcctg cacaagctgg
     1501 ccgtagctga tcgtcttgcc gccgccggag acgacgccgt ttgtgacgct gaggccggtg
     1561 accggcacgc cgagcttctg cgaggccagc gcgagcagcg cctggtaggt gtaggcggcg
     1621 gccttcatga tgttcgggcc gccgaggaac atcatccccg cggcgaggcc gccgtcaggc
     1681 gtctcgtcgg tgctggccct gatcaccttg gtgatctggg agtaggagac gttcagctgc
     1741 tccgcggcga actgcgcgaa cgcggtgctc gccgagccct gtcccatctc ggcgatgccg
     1801 gtgcgcatcg tgatcgtgtt gtcgggatgg atcgtgaacc aggaggcggg ctgggtcggg
     1861 tcgagggagt gggtgatgct gtcggcctgc gcggccttcg caacgatgcc cgagccgacg
     1921 aggcttgccc cgacgacgag cgcgccggcg cccttgacga agcgcgagcg tgtcaacgtg
     1981 gcgccgtaga cgtcgagcac cgtttcctcc tgcccgcttt cgttcggggc aacgtgcgtg
     2041 gtctcagagc gtgccatctc aggctccctt cgccatcgtc gcggcggcca tctggatggc
     2101 ctcgatgatc gcggtgtagg ttccgcagcg acagaggtgg acgttcatcg ccacctggat
     2161 ctgctcgcgg gttgggttct tggtcgtgcc gagcaggtcg gctgcctgaa tgatcatccc
     2221 gctctggcaa tacccgcact gtggcacctg aacatcgatc cacgcctgct gcagcgggtg
     2281 cagcacatcg acggttgccg cggtaccacg ctttgccgcc cagacggccg gcaggccctc
     2341 gagcgtcgtg atctggccgc cggtcaccgc cgcgacgggc gtgacgcagg agcggatctc
     2401 cttgccgttc agcagcaccg agcaggcacc gcactgggca agcccgcagc cgaagcgcgg
     2461 gcccttcagg tgcagctcgt tgcgcaggat gtacaggagc ggtgtgtcaa ggcccgcact
     2521 gaccgtgtgc gtaaggccgt tgaccttgat cttgagcgta ctcgccatgc cccctcctcg
     2581 tcgaaatcgc cgcgcattgc gaccggggcg gtcggcgcgc gctgactcgg gaacgacgct
     2641 cggagcacac gcctcgtccc ggtagaacaa tctgccgaaa ctaccacgac tctctccaag
     2701 attgcaagac ctccaatttg cacgccggca ggccggggcc ggtgctaccg ttcccgcaag
     2761 cgtgtcggcg atccccgagg gggcgagatg atgaagcagc gcggcagggc actaggtcgg
     2821 agcgtggtcg tgcgcggatc ggtggcgctc ggaggcctct gcacgctcgc ggctctggtc
     2881 gcggtcgcgc ccgccggggc cgcgctgcgg gcccacggca ccgcggcgct cgtgaccgtg
     2941 acggtcagcg acagcgccat gcgcgtctcg gcgacgagcg cctcctccgg ccccacgacc
     3001 ttcgtgctcg tcaaccacgg caagaagctg cacgcgcttg tcatctccgg cccgggcgta
     3061 aagaaggcac agacccagaa ggtcacggcc ggccacagcg ccgagatcac ggtcacgctg
     3121 cgccccggtg cttacgagct ctcggacccc gtcggcctcg gcgcctacga cgtccagttc
     3181 ctggacgcga tcccggccac aaccgtgagc gccaccggct cgagcgccgt cgtgacgccg
     3241 cccgcgccgc tgccgccgat gtgcggccag tactactccc cctagccggg gcgcggccac
     3301 gcgtgcgggg cgcaagggcc tcgaggctgc ttgttttttt gctttgctac ggcgccggcg
     3361 tgacgacgcg gacgacctcg cccggcggcg tcgcccggcc ggtcgcggtg ccgtcgttgg
     3421 agacgtag
//
LOCUS       DASWVF010000013         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_408059, whole genome shotgun sequence.
ACCESSION   DASWVF010000013 DASWVF010000000
VERSION     DASWVF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408059"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGD55_00225"
     CDS             complement(<1..409)
                     /locus_tag="VGD55_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0868799.1"
                     /translation="MEAEMITMNRSDVLVDASWLAQHLHDTGLRIVEIDVSPAAYRAG
                     HIPGALLWNVYADLKDANYQLLDKSAFSQLLSRSGIDADSTVVFYGYAPAMGVWLMKL
                     YGHVDVRVLDCARDVWENDGLPWDAEVPTPTPTL"
     gene            complement(414..572)
                     /locus_tag="VGD55_00230"
     CDS             complement(414..572)
                     /locus_tag="VGD55_00230"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEY0868800.1"
                     /translation="MARIIRCECGFVARGGTDDEVISTIRGHMSTDHPALLESVGRED
                     LLGWIQVE"
     gene            622..1377
                     /locus_tag="VGD55_00235"
     CDS             622..1377
                     /locus_tag="VGD55_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0868801.1"
                     /translation="MTLASALPSRIQLCGPTVVELGGQRVEALLPGRQGRTLFAYLVL
                     NRHRLASRDELFAALWPSRPPAGSETAFNALLSKLRKAIAPVVIDGRSSLRLQLDDDA
                     WVDVEVAEAAVHRAESRIVLGDSKSAWGASLAALFIAEREFLPGDEAPWIADQRARMA
                     QVRRRSLEAYASAALGTGGTELPAAVRAGRQLVRIDPLRETGYQILMQALAAQGNSAE
                     ALRVYADLCEVLRDELGVSPSAPIEAVYQSLLR"
     gene            1503..>1858
                     /locus_tag="VGD55_00240"
     CDS             1503..>1858
                     /locus_tag="VGD55_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868802.1"
                     /translation="MSGNWERPPASTRSPSTTRPWLVAIASAAAVFVVVVVVGLAVGS
                     GRDRSASSSSGVFPRPTTYSSLTPSLFQTVLPSASPGVASNQVPAPSPTTATAQAVDT
                     AAPLPTPTGPVPTAPAG"
BASE COUNT          303 a          605 c          611 g          339 t
ORIGIN      
        1 acagcgtggg ggtcggcgtc ggcacctcgg cgtcccatgg aagcccgtcg ttctcccata
       61 cgtcgcgcgc gcaatcgagc accctcacgt ccacatgacc gtagagcttc atcaaccaca
      121 cccccattgc gggcgcgtag ccgtagaaca cgacggtcga gtcggcgtcg atgcccgacc
      181 gcgaaaggag ctgcgagaag gccgacttgt cgagcagttg gtagttcgcg tccttgaggt
      241 cggcatacac gttccacagc agcgcgcccg gaatgtgtcc cgcgcgatat gcagcagggc
      301 tcacgtcgat ctcgacgatg cgaagcccgg tgtcgtgcag atgctgggcg agccacgaag
      361 cgtcaacgag gacgtcgctg cggttcatag tgatcatttc ggcctccacg tggttactcg
      421 acctgaatcc agccgagcag gtcttcgcgc ccgaccgatt cgaggagggc gggatggtcg
      481 gtgctcatgt gaccgcggat cgtggagatc acctcgtcgt cggtgccacc ccgagcaacg
      541 aagccacatt cgcagcggat gatccttgcc atgtcgtcct cctagaacgt tgtaggcctg
      601 cgtacttggc agaatgttgg cgtgaccttg gcgtcagcgc tacccagcag gatccagctc
      661 tgcgggccga ccgtggtcga gctcggtggg cagcgggtcg aggctctact gccgggccgc
      721 caagggcgaa cgctgttcgc ctacctcgtg ctcaaccgtc atcgactggc aagccgcgac
      781 gaactcttcg cggcactttg gccctcgcga cctcccgcgg gcagcgagac cgccttcaac
      841 gccttgcttt ccaagttgcg caaggcaata gctcccgtcg tgatcgacgg ccgttcaagc
      901 cttcgacttc agttggacga cgatgcgtgg gtcgacgttg aagttgccga ggccgcagtc
      961 caccgcgccg agtcacgcat cgtgctcggc gattccaaga gcgcgtgggg tgcgtcgctc
     1021 gccgcgttgt tcattgctga gcgcgaattc ctgcccggtg acgaagcgcc gtggattgcc
     1081 gaccaacgtg ctcggatggc tcaggttcgc cggcgctcgt tggaggcgta cgcgtcggcg
     1141 gctctgggaa ctggcggtac cgaattgccc gcggccgtgc gagcaggtcg gcagctggtg
     1201 cggatcgatc cgctgcgtga gaccggctac cagatcctga tgcaagccct cgccgcccaa
     1261 gggaattcgg ctgaggcgct tcgggtgtat gccgacctgt gcgaggtgtt gcgcgacgag
     1321 ctcggggtgt cgccgagcgc ccccatcgag gccgtctacc aatcgttact ccggtgaacc
     1381 ctaagttgta agcctctgga cgctcaagag agcgctgacg agcttccaac taaggcaggg
     1441 tggggctgga accgggcgaa cggtcgttgc gtctgaacgc caggttcggc ggaaggatca
     1501 ccatgagcgg caactgggag cgtccaccgg cctcgacgcg gtccccgtcg acgacgcgtc
     1561 cgtggctcgt cgcgatcgcg tcggcggccg ctgtctttgt agtcgtcgtg gtggtcggcc
     1621 tcgcggttgg cagcgggcgc gatcgatcgg cgtcatcgag ttccggcgtt ttccccaggc
     1681 cgaccacgta ctcgagcctc acgcccagcc tgttccagac agttctgccg agcgcatcgc
     1741 cgggtgtcgc gtcgaaccag gttccggcgc cctcgcccac cactgcgaca gcacaagcgg
     1801 ttgacaccgc tgccccgttg ccgacaccaa ccggtccggt gccaacggcg ccggccgg
//
LOCUS       DASWVF010000014         4888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_433582, whole genome shotgun sequence.
ACCESSION   DASWVF010000014 DASWVF010000000
VERSION     DASWVF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433582"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1041
                     /locus_tag="VGD55_00245"
     CDS             73..1041
                     /locus_tag="VGD55_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868803.1"
                     /translation="MADASVEVVDLVKRYPKRDSNAVDGISFRVASGECFGLLGPNGA
                     GKTTTIGVLTTRVKPTRGSAFVAGVDVVAYPVRARTQLGVVPQRPNLDRSLDVRQNLL
                     FHASYHGVPVRIREARAIELLEQFGLADRATDKVDRLSGGQLQRVMIARALMHSPPVL
                     FLDEPATGLDPAARIYVWDRIRDLKSSGVTILLTTHDMDEAAALADRVGIVDHGHLLA
                     LDTPVALTKTLIGSGTLEIVVESVGDSAALVGAFERLDAVERVERVGEGGPVLRARLY
                     VTGEPARIVAPVAEIVTRHQGRLTEVRLGEPSLEDVFIHLTGRALR"
     gene            1038..1853
                     /locus_tag="VGD55_00250"
     CDS             1038..1853
                     /locus_tag="VGD55_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868804.1"
                     /translation="MTTASVALPRVEVTDPSAVRSFLSVLRRDIVVTGRELPIFLAQV
                     ILQPLGLLFIFGKVLGSIGLTTPGYAHLLFPGIVALTVTLTGIQSTALPLVLEFSFSR
                     EIEDRLLAPLPTWAVAIEKMLFASMRALLAALVMFPVGIWVLGSIPWHASAIWLVAVV
                     MILGALAGSAIGLTLGTLVPPNRIQIMFALILTPMLFTGCSQYPWPSLSHLRWFQVVT
                     AFNPMTYASEGMRAALTPSVHHMRPWICVLGLTISVVVFTFVGVRGFLRRAID"
     gene            1938..2486
                     /locus_tag="VGD55_00255"
     CDS             1938..2486
                     /locus_tag="VGD55_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868805.1"
                     /translation="MRAQRKYALKGMAGTAFVALAITGCASSSKPAASSAATGGTASG
                     AAVVATADGTMGAFLTDGAGRTLYLWLGDSSSTSMCTGQCATYWPPLLTSGAPTANGM
                     ADSKLLATSKRADGKTQVTYAGHPVYYFSGDKTAGATDGQGQNAFGYLWWVVSPAGAG
                     ITSGGSSSSSSPSATAPAVRGY"
     gene            complement(2496..3209)
                     /locus_tag="VGD55_00260"
     CDS             complement(2496..3209)
                     /locus_tag="VGD55_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868806.1"
                     /translation="MVDEPIIGRTVAMACFMALVTACASQRTTPPHSALRDSAPVSAY
                     ETETATPAPPTGAHPVPPEIPCVYPADDPPAAWDPGFVGVLQESDVVVEADIEDKFQQ
                     LGQDGDQVFWSQLLDNVDVLRSRATPAPTVVGLDAQGDPGEPPYAWPTGRYVLLLLAP
                     RNGLSSPSDGMFGMFRIVDGHALRFCPNYDDPAHPIAASGTPPTVEQLLALIPPELPS
                     DIVSPKPGAATVSPAMSSS"
     gene            3352..4749
                     /locus_tag="VGD55_00265"
     CDS             3352..4749
                     /locus_tag="VGD55_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY0868807.1"
                     /translation="MARELADVAAMAPSVELASLLAGVTPDLVDDDNDLLEVIAGWDR
                     LASWVTAHQLVAVAEFARRPWSTGVGPDTAVARAKRAPVGGVGRDFPDDEIAARLAIT
                     SGSAGFKLSLAVTLASPLTATADALRAGVIDVQKARAIADGCRHLDPATAVEVETTVL
                     ARAAGQTAATVRKAVRRAAIAADPVAAAARCASMKDERGVWLIPQDDGVTELRALLSA
                     PDALSVYQVLTAAGAAAKTVGGEARTIDQLRADYLVAPFMAAIASGELAGAVPARLAS
                     HHGQRGQLQLTVPASVVMGVSNAPGELVGYGPITAEVARVLAGDCTWRRIVTNPIDGT
                     FLDADSKTYRPPAALVRHVEGRDQTCVFLGCDRPAAKCHLDHTTRFPAGETRHDNLGA
                     MCRRHHLLKHALDGAFANLKQPTPGTFEWTLPTGHVYARQRPPIAPSAEAETGIDPPA
                     ILRKAADPTGDDPPF"
BASE COUNT          772 a         1509 c         1648 g          959 t
ORIGIN      
        1 ggcaactcaa ccagtcttga ggccgtcgca tcgcggtcgg gttacagttg ccggtcggta
       61 acgggaggtg ttatggcgga cgcgtcagtc gaggtcgtcg acttggtgaa gcggtatccg
      121 aagcgcgact cgaacgcggt cgacggcatc tcgtttcggg tggcgagcgg tgaatgcttc
      181 ggtctcctcg ggcccaacgg agcaggcaag acgacgacta tcggtgtgct caccacgcgg
      241 gtcaagccga cacgtgggtc tgcgttcgtt gccggcgtcg atgtggtggc ttaccccgtc
      301 cgcgcgcgca ctcagttagg tgtagttccg caacgtccca atctcgaccg ctccctcgac
      361 gtccggcaga atctgctctt tcatgcgagc tatcacgggg tgccggtgag gatccgggag
      421 gctcgggcca tcgaattgct cgagcagttc ggccttgccg accgcgcaac cgacaaggtc
      481 gatcggcttt cgggtgggca gctgcagcgc gtgatgatcg cccgtgcgct gatgcattcg
      541 ccaccggtgt tgttcctcga cgaaccggcg accgggctcg acccggccgc gcggatttac
      601 gtgtgggaca ggattcgtga cctgaagtcg tccggtgtca cgatcttgct gacgacgcac
      661 gacatggacg aggccgccgc gcttgccgat cgcgtcggca tcgtcgatca cgggcacctg
      721 ctcgccctcg acactccggt cgcgctcacg aagaccctca tcggcagcgg cacccttgag
      781 atcgtcgtcg agtcggtggg cgattcggcg gcgttggtcg gggcgttcga gaggctcgat
      841 gcggttgagc gcgtcgagcg tgtcggcgaa ggcgggccgg tgcttcgcgc ccggttgtat
      901 gtgaccggcg agccggcgcg gatcgtggca ccggtcgcgg agatcgtgac gcgccatcag
      961 gggcggctca ccgaagttcg gctcggagaa ccgagtctcg aagatgtctt cattcacctg
     1021 acgggacgtg cgctgcgatg accacagctt ctgtcgccct tccgcgagtc gaggtcactg
     1081 acccttctgc ggtgcgttcc tttctgagtg tcttgcgccg cgacatcgtc gtcacgggac
     1141 gtgaactgcc gatctttctc gcgcaggtga ttttgcagcc gctgggtttg ctgttcatct
     1201 tcggcaaggt ccttggctcg atcggactca cgactccggg atatgcgcat ctgctctttc
     1261 ccggcatcgt ggcgttgacg gttactctca ccgggattca aagcacggca ctgccgttgg
     1321 tgctcgagtt ttcgttcagc cgcgaaatcg aagaccggct gcttgcaccc cttccgacat
     1381 gggctgttgc tattgagaag atgctgttcg cgtcgatgcg cgcattgctc gcggccctgg
     1441 tgatgttccc ggttggcatc tgggtactcg gctcaattcc gtggcacgcc agtgcgattt
     1501 ggcttgtcgc ggtggtgatg attctcggtg cgctcgccgg ctctgcgatt gggctcactc
     1561 tcggcacgct cgtgccgccg aaccggatcc agatcatgtt cgccttgatt ctcacgccca
     1621 tgttgttcac cggctgtagc caatatccgt ggccgtcgct gtcacacctg cgctggtttc
     1681 aggtggtcac cgcgttcaat ccgatgacgt acgcaagcga aggcatgcgt gctgcgctga
     1741 cgccctccgt gcatcacatg cgaccgtgga tttgcgtgct cggtctcact atctccgtcg
     1801 tggtattcac ctttgtgggc gttcgcggat tcttgcgacg cgcgatcgac tgaacgggtt
     1861 gggccgcccg cacgtggggt aggtgggcta cacgtcggcc ctcgcggaaa cgcgccgatg
     1921 ttttgatggg acgaatcatg cgtgcacaac ggaaatacgc gttgaagggc atggcaggca
     1981 ccgctttcgt ggcgctggcc atcacggggt gtgcgagcag ttcgaagccg gcggctagct
     2041 ccgccgccac tggcggcacc gcgtctggtg ccgcggtcgt ggcaacggcg gacggcacga
     2101 tgggcgcatt cttgacggac ggcgctgggc gcactctcta cctatggctg ggtgactcgt
     2161 cgtcgacgtc gatgtgcacg gggcaatgcg ccacctactg gccgccgctg ctcacctcgg
     2221 gagccccgac ggcgaacggc atggccgaca gcaaactgct cgccacttca aagcgcgccg
     2281 acggtaagac gcaggtgacc tacgcgggtc acccggtgta ctacttctcc ggagacaaga
     2341 ccgcgggcgc gacggatggg cagggtcaga acgcgttcgg ctatctgtgg tgggttgtct
     2401 caccagccgg tgcgggcatc acatctggcg gttcgtctag cagctcgtcg ccatcggcta
     2461 ctgcgccggc ggtgcgcggg tactgagtcc gtcgcctagc tgctcgacat cgcggggctg
     2521 accgttgcgg cgccgggctt ggggctcacg atgtccgacg gtagttcggg cgggatgagt
     2581 gcgagcaact gctcgacagt tggcggcgta cctgatgcgg cgatcgggtg cgctgggtcg
     2641 tcatagttcg ggcaaaacct gagtgcgtgg ccatcgacga tgcggaacat gccaaacatg
     2701 ccgtcgcttg gggaactcaa gccgttccga ggcgcaagca gtagaagcac gtagcgaccg
     2761 gttggccagg cgtagggagg ctcgccgggg tcgccctgcg cgtccaaccc gacaacggtc
     2821 ggagccggag tcgcgcggct gcgcagcacg tcgacgttgt cgaggagctg cgaccagaag
     2881 acctggtcgc cgtcctgacc caactgctga aacttgtcct cgatgtcggc ctcgaccacg
     2941 acatcgcttt cttgaaggac gccgacgaac cctggatccc atgccgcggg tgggtcgtcg
     3001 gcgggataga cacacggaat ctcgggtggg acgggatgag ctcccgttgg cggcgcaggt
     3061 gtcgcagtct cggtctcata agcactcacc ggcgcgctat cacgcagtgc agagtgcggg
     3121 ggcgtcgtgc gctgtgacgc acaggccgtc acgagtgcca tgaagcacgc catcgcaact
     3181 gttcgtccga tgatgggttc gtcgaccatg agcgccagcc tctcgccgtc cagaacagtt
     3241 tgtcacgcat tggacggcga gtgcgcgcgt cgggttgcat cgcaaatcct ttgtccacag
     3301 cgttgttgct cgttccaagg atctgtcggt ggcctcagat actttcggtt catggcaagg
     3361 gaattggcgg acgtcgcggc gatggctcct tcggtggagt tggcgtcgct gcttgctggg
     3421 gtaacgcccg atctcgtcga cgatgacaac gacctgctcg aggttatcgc cggctgggat
     3481 cgcctcgcgt catgggttac cgcgcatcag ttggtggcgg ttgcggagtt cgcgcggcgt
     3541 ccgtggtcga ccggcgtcgg gcctgacact gcagtggcgc gggctaagcg cgcaccagtg
     3601 ggtggagtgg gtcgcgactt tcccgacgac gagatcgccg cgcggttggc gatcacgtct
     3661 ggctcggcgg gcttcaagtt gtcgctcgct gtgacattgg cgtcgccgtt gaccgcgacc
     3721 gccgatgcgt tgcgggccgg tgtgatcgac gtgcagaagg cgcgggcgat cgccgacggc
     3781 tgccgacacc tcgatccggc gaccgcagtc gaggtcgaaa cgacggtgct ggcaagagct
     3841 gcggggcaaa ccgcggcgac tgtgcgcaaa gccgtacgga gagcagctat cgcggccgat
     3901 ccggtggcgg cagccgcccg atgcgcatcg atgaaagatg agcgcggtgt gtggctcatc
     3961 ccacaagacg atggcgtcac cgagttgcgg gcgttgttga gcgctcccga tgcactcagc
     4021 gtctaccagg tgctaacagc cgcgggcgcg gccgcgaaga ccgttggcgg cgaggctcgc
     4081 acgatcgacc agctgcgcgc tgattacctc gtcgcgcctt tcatggcggc gatcgcgagc
     4141 ggcgagctcg ccggtgcagt gccggcgagg cttgcttctc accatgggca gcgcggtcag
     4201 ttgcaactta ctgtgcctgc gtcggtggtc atgggagtgt caaacgctcc cggtgaactc
     4261 gtcgggtacg gcccgatcac ggccgaggtt gcccgcgttc tggctggcga ttgcacgtgg
     4321 cgtcggattg tcaccaaccc catcgacggc accttcctcg acgctgactc gaagacgtat
     4381 cggccaccgg ccgctctggt caggcatgtc gagggccgcg accagacctg cgtgttctta
     4441 ggctgtgaca ggccggcggc gaagtgtcac ctcgatcaca ccactcgctt tccggcaggc
     4501 gagacccgcc acgacaacct cggcgcgatg tgtcgtcgcc accaccttct caagcacgcg
     4561 ctcgatggag catttgcgaa cctgaaacag cccacgcccg gcacgttcga atggacattg
     4621 cccaccggac acgtctacgc caggcaacgc cccccgattg ctccgtcagc ggaggcagaa
     4681 accgggatcg atccgcccgc gatcctgagg aaggctgccg atccgacagg cgacgatcct
     4741 ccgttctagg gcggggcggt cccttcaatg ctgcggcacc ttgaccgcat ctcatgcgac
     4801 ctggtcgaga aaccgatcga ccagagtcac agcacgctcg cgtgccgcgt cgacatcgag
     4861 tcgtgtgcgc tccatcaact cgtcgggg
//
LOCUS       DASWVF010000015         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_357169, whole genome shotgun sequence.
ACCESSION   DASWVF010000015 DASWVF010000000
VERSION     DASWVF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357169"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGD55_00270"
     CDS             <1..496
                     /locus_tag="VGD55_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0868808.1"
                     /translation="DAGVWHAVFPAMAANIGRDGFSADKHEGDLRTPAGKYGFGTMFG
                     QQASPGVKFPYRLADSQSVWVDDVSSKYYNTWQENAALTGEHLDSATYRTAYAYAVDI
                     AYNTSPVVPGKGSAIFLHVGDGGGTAGCVSIAQPNLLKVLRWLDPARSPVIVMGPTSV
                     IRQY"
     gene            complement(493..1401)
                     /locus_tag="VGD55_00275"
     CDS             complement(493..1401)
                     /locus_tag="VGD55_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0868809.1"
                     /translation="MADPTFVEAAGGVLWRRASGGLGVEVALVHRPKYDDWSVPKGKL
                     LPDEHPLVGALREVVEETGYTGPPGRLLGESRYLKDGSPKRVRYWSIEATEGSFVTNA
                     EVDQVMWLPPREAAVHLNPDRDQRIVLEFTRDVRVTTACVVVRHGSAGERSSFDGDDR
                     ERPLDDLGHRQALALIPVLAALRVERVLSADVLRCLDTVGPYAAQRRLSLQSEPLLSE
                     SGYAGAPDAAADRLVALLAADATPTTVCSQGRVIPPLLQRVCMELKAPSPWDGSVPKG
                     GFVVLNMAANADTNTIVSVDKYRPIA"
BASE COUNT          291 a          517 c          462 g          245 t
ORIGIN      
        1 agacgctggc gtctggcacg cggttttccc agcaatggca gcaaatatcg ggcgcgatgg
       61 cttctcagcc gacaaacacg aaggagacct gcgaaccccc gcgggcaagt acggcttcgg
      121 cacgatgttc ggccaacaag cgagcccggg cgtcaaattt ccctaccggt tagcagattc
      181 acagtcggtc tgggtcgatg acgtctcatc gaagtactac aacacgtggc aggagaacgc
      241 cgcgctcacc ggcgaacatc tagacagcgc gacctaccgg accgcttacg cctacgcagt
      301 cgatatcgcc tacaacacaa gtccggtagt gcccggcaag ggcagcgcga tcttcctcca
      361 cgtcggcgac ggcggcggga cggcgggctg cgtttcgatc gcgcagccga acctgctgaa
      421 ggtgctgcgc tggctcgacc ccgcacggtc accggtgatc gtgatgggtc cgacgtcagt
      481 gatccgccag tactaggcga tcgggcggta cttgtcgacc gacacgatcg tgttcgtgtc
      541 ggcgttcgca gccatgttga gtacgacgaa gccgcccttg ggcactgagc cgtcccatgg
      601 gctcggagcc ttcagctcca tacacacgcg ttgtaacagc ggcggaatca cccgaccctg
      661 actgcacacc gtggtcggcg tcgcgtcggc cgccagtaac gcgaccagcc ggtcggctgc
      721 agcatcagga gcgccggcgt agccagattc ggacagcagc ggttcgcttt gcaacgacag
      781 ccggcgctgc gcagcgtacg gcccgacggt gtcgaggcag cgcaacacgt cggccgacag
      841 cacccgttcg acgcgaagcg cagcgagcac cgggatcagc gcaagcgcct gccggtgacc
      901 caaatcatcg agtggccgct cacgatcgtc accgtcgaac gacgagcgct cgcccgcact
      961 gccgtgtcgc acgacgacgc acgcggtggt gacccgaaca tcgcgcgtga actctagaac
     1021 gatgcgctga tcacggtcgg ggttcagatg cactgccgct tcgcggggcg gcagccacat
     1081 cacctggtcg acctctgcat tcgtcacaaa cgaaccctcg gtcgcttcga tcgaccagta
     1141 acggactcgc ttcggtgacc cgtccttcag atagcggctt tcaccgagta ggcgaccagg
     1201 tgggccggtg tagccggtct cctcgaccac ttcacgcagc gcgccgacga gtgggtgctc
     1261 atcgggcaac aacttgcctt tgggtactga ccagtcgtcg tacttgggcc ggtgcaccag
     1321 cgcgacctca acgccaagac cacccgaggc tcgacgccat agcacgcccc cagcagcctc
     1381 aacgaacgtc gggtccgcca tcagtgagag acgccgatca actggacagc caacgacggc
     1441 gcttggccct cacgacgtcc ggccatatct cgccgaactc gaccctcgct gccgccgctc
     1501 gggcttcttc gcgtg
//
LOCUS       DASWVF010000016         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_51483, whole genome shotgun sequence.
ACCESSION   DASWVF010000016 DASWVF010000000
VERSION     DASWVF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51483"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VGD55_00280"
     CDS             complement(<1..1362)
                     /locus_tag="VGD55_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY0868810.1"
                     /translation="MAVTFAVGAVNANPRTLAVWCPDWPVIAAGGSFDVPGAVLHANR
                     VVACSAAARLEGVRRGQRRRDAQSKCPELTVYGHDPGRDARLFESVAAAVEAIVPGIE
                     IIRPGLLVVAARGAARYYQGELPLLATIATAVVDATRYDVQLGIADGIFAATQAAYAA
                     HIVPAGEAAAFLAPLPIRVLDRPDLADLLVRLGIRTLGEFAALPRIDVLARFGPDGAY
                     VHDLARGVEARPPNARVAPPELAVEMTLDPPVERVDTATFVAKTLAEQLHERLADLGL
                     ACIRISIEACTENGEQLSRSWRHGSASTAGFTAAAIADRTRWQLDGWLSGTISSRDGR
                     PSSGLVFLRLVPDEVVYDDGRQLTLWGAEAGSGPGGDLEQIDRALSRVQGILGPDAVV
                     TAVVGGGRDVSEQIRLVSWGEPRDVLLADAYPWPGRLPAPSPATVFRSAQPAAVTDAD
                     GLPV"
     gene            complement(1353..1871)
                     /locus_tag="VGD55_00285"
     CDS             complement(1353..1871)
                     /locus_tag="VGD55_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868811.1"
                     /translation="MPQALAELFPEGGLRRGSTITVSGSSSLVLALLAEISRKQGWCA
                     EVGSPLLGSAAAAEAGVTLERFVRVAEPGEQWPSIVASLLEAFDIVVVHPPPRAREAD
                     MRRLSARARERSAVLLATTKWDGALLGLSVTDQRWHGLGSGHGYLRARELDVQALGRG
                     AATQPRRTTVWL"
     gene            2016..2507
                     /locus_tag="VGD55_00290"
     CDS             2016..2507
                     /locus_tag="VGD55_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0868812.1"
                     /translation="MASTDLHPNAVRIQASLIAAGATGEVRMLDESARTAAEAAAALG
                     VEIGQITNSLIFVANGEPLLVLTSGRHRVDTAKVAELVGVTKVGRADADFVREHAGVA
                     IGGVSPIGHPRPLRTLVDVALGDYDVVWAAAGHTHAVFPTTFDELVAITNGTPAQVGV
                     QPE"
BASE COUNT          498 a          919 c          836 g          382 t
ORIGIN      
        1 aaccggtaag ccgtcagcat cggtgaccgc ggccggctgt gccgacctga agacggtggc
       61 cggtgatggt gccggcaacc gacctggcca cgggtatgca tcggcgagca gcacgtcgcg
      121 tggctcaccc cacgacacca acctgatctg ctcgctgacg tcacgcccgc caccgacgac
      181 cgcagtcaca accgcatctg gcccgaggat gccttgcacc cgcgacagtg cgcggtcgat
      241 ctgttcgagg tcaccaccag gaccagaccc agcctcggcg ccccacaacg tgagctgccg
      301 gccgtcgtcg tagacgactt cgtcgggcac caaccgcaag aacaccaggc cactcgacgg
      361 ccgcccgtca cgtgaagaga tcgtgcccga caaccaaccg tcgagctgcc agcgggtgcg
      421 atctgcaatc gcggccgcgg tgaatcctgc ggtcgaggca cttccgtgcc gccagctccg
      481 cgacagctgc tcgccgtttt ccgtacacgc ttcgatgctg atgcggatgc aggcgagacc
      541 aaggtcggcc aggcgttcgt gcaactgctc tgccagggtc ttggccacaa aggtggcggt
      601 atcgacacgc tctaccggtg ggtcgagcgt catctcgaca gccagctcgg gtggcgcgac
      661 ccgagcgttc ggcggccgag cctcgacacc gcgagcgaga tcgtgcacgt aggcgccgtc
      721 tggaccgaac ctcgcaagca catcgatgcg tggcaatgcc gcgaactcgc caagggtgcg
      781 gatgccgagc ctcaccagca gatcggcaag gtctggccga tcgagaacac ggatcggcag
      841 cggtgcaagg aaagctgccg cctcgccagc aggcacgatg tgcgccgcat aagcagcttg
      901 ggtcgcagcg aagatgccgt cggcaatacc aagttggacg tcgtacctgg tcgcatcgac
      961 taccgcagtt gcgatcgtcg cgagcaacgg caattctcct tggtaataac gcgccgcacc
     1021 tcgcgcagcc accacgagca gcccgggcct gatgatctcg atcccaggaa cgatcgcttc
     1081 gacggcagca gcgaccgact cgaaaagccg ggcgtcacga cctggatcgt ggccgtagac
     1141 cgtgagctca ggacacttcg actgcgcatc gcggcgacgt tgccctcgac gcactccttc
     1201 gaggcgagca gcagccgaac aggcaacgac gcggttggcg tgcaggaccg caccaggaac
     1261 gtcgaagctg ccgccggcgg cgatcaccgg ccagtcggga caccagaccg caagagtgcg
     1321 cgggtttgcg tttacggcgc cgacggcaaa tgtcacagcc ataccgtggt tcgcctcggt
     1381 tgggttgccg cgccacgacc gagtgcttgc acgtcgagtt cgcgagcgcg caggtaccca
     1441 tgccctgacc cgagcccgtg ccagcgctga tcggtgacag aaagccccag cagcgcgccg
     1501 tcccacttcg ttgttgctaa caacaccgcc gaccgctcgc gagcccgcgc gctcagccgg
     1561 cgcatatcgg cttcgcgggc ccgcggcggc ggatggacga ccacgatgtc gaaggcctcg
     1621 agcaggctgg ccacgatcga gggccactgc tcccccggct ctgccacccg gacgaaccgc
     1681 tcgagtgtca cccctgcttc ggccgccgcg gcactgccga gcagcggcga accgacctcg
     1741 gcacaccagc cctgcttgcg cgaaatctcg gcaagcaaag ccagtacgag ggacgacgaa
     1801 ccgctcacgg tgatggtcga accccggcgt agcccccctt cgggaaaaag ctcggccagc
     1861 gcttggggca ccggcaaaag ctcccgggcc tcaaccccac ggagtcgctc gaggaggtcc
     1921 tgtggaagag cctctagcga cgatgcggct gagcggagca tcccttgagt atcgaacata
     1981 tgttcgaacg ccgtcaacag acctatcctg tgcgagtggc gagcaccgac ctgcatccca
     2041 acgccgtccg cattcaggct tccctgatcg ccgcaggcgc gaccggcgag gtgcgcatgc
     2101 tcgacgaatc ggcccgcacc gccgctgaag ccgctgccgc gctcggggtc gagataggtc
     2161 agatcaccaa cagcctgatc ttcgtagcga acggcgaacc gttgctcgtc ctgacaagtg
     2221 gccgccatcg ggtcgacacc gcgaaggtgg ctgaacttgt cggcgtgacc aaggtcggtc
     2281 gggccgatgc cgacttcgtg cgcgagcacg ccggcgttgc gatcggcggg gtatccccca
     2341 tcggtcaccc gcgcccgctg cgcaccctcg tcgacgtcgc cctaggtgac tacgacgtcg
     2401 tctgggccgc ggctggccac acgcacgcgg tcttccctac gacattcgac gagctcgtcg
     2461 cgatcacgaa cggcacgccg gcacaggtcg gcgtccaacc cgagtgacca tcgggcaacc
     2521 ccggggaaca ggtgccgtag ggcagaatgg cgctatggct gctgcgaaga acactccgtc
     2581 cgaatccgag ggcacggcgg taaccgacga gacggtcacc gtgacaactg ccgca
//
LOCUS       DASWVF010000017         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_229915, whole genome shotgun sequence.
ACCESSION   DASWVF010000017 DASWVF010000000
VERSION     DASWVF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229915"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGD55_00295"
     CDS             complement(<1..624)
                     /locus_tag="VGD55_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0868813.1"
                     /translation="MPSRADLLVDATSLAAEIASDRPPVVIDVRWSLAGPPGIAAYRA
                     GHLPGARFADLDTELSGRRGAGGRHPLPESADFEVLMRRLGVNGDSAVVCYDAADSVP
                     ASRAWWELRYFGHDHVRVLDGGYAAWVAAGLRTTTDEPSAPEGNFVPRPGGLPMLDAD
                     EAGHLAEHGMLVDVRAGERYRGEVEPVDAVAGHIPGAVNVPTVGNIGP"
     gene            complement(649..1314)
                     /locus_tag="VGD55_00300"
     CDS             complement(649..1314)
                     /locus_tag="VGD55_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0868814.1"
                     /translation="MSSQSGMRQHGMSSVKCQISISVDGFVAGPNQSLENPIGEGGMR
                     LHEWVFGTASWRELQGLDGGSPSADSDVISEAMAGIGAHIMGRKMFGGGDGPWDETWT
                     GWWGDDPPFHKDVFVLTHHERAPLSLHDTTFFFVRDGVESALKQARASADGKDVAIAG
                     GGSVVRQYLAAGLLDELYLHIVPIVLGAGERIFDDVGDVVFEPANVVASPGVTHIKYR
                     VVR"
     gene            complement(1311..1736)
                     /locus_tag="VGD55_00305"
     CDS             complement(1311..1736)
                     /locus_tag="VGD55_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868815.1"
                     /translation="MTPIALREVEAAVRDAWAADTCDPVDVADWSPDNPARGQCGSTA
                     LVLHDLLGGVLLLAEVRNADGSLQGYHYWNRLADDIEVDLTGEQFHTSEAVREPKVVV
                     RTTALPARGAEQYLLLRARVTAALAANTVNEVTTPGIGR"
     gene            1972..>2370
                     /locus_tag="VGD55_00310"
     CDS             1972..>2370
                     /locus_tag="VGD55_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868816.1"
                     /translation="MSAINPELGWELGPGHKAQHVLCVTAGGVAAVRPTAERWLRAAP
                     PVDSAWEYIAAKAAHPLPLDDKLVLGTWTLNIAETRFGIVVEESRLEVDVTCFHPHFP
                     VMPEDARNEVMFIVLDWLLGEDDIERWVGHL"
BASE COUNT          440 a          787 c          743 g          400 t
ORIGIN      
        1 agggccgatg ttgccgactg tcggcacgtt gactgcgccg ggtatgtgac ccgcgaccgc
       61 atcgactggc tcgacctcac cgcgataccg ctcccccgct cggacgtcga ccagcatccc
      121 gtgctcagca aggtggccgg cttcatcagc gtcaagcatc ggcaacccac ccggtcgggg
      181 tacgaagttg ccctcgggcg ctgatggttc gtcggttgtg gtgcgcaagc cggcagcgac
      241 ccacgccgca tacccgccat cgagtactcg cacgtggtcg tggccgaagt agcgcaactc
      301 ccaccacgca cgcgacgcag ggacagagtc cgccgcgtcg tagcagacca ctgcgctgtc
      361 accgttcacc ccgaggcggc gcatgagcac ttcgaaatcg gcagattcgg gaagcgggtg
      421 gcggccacca gcaccgcggc gtccggacaa ttctgtgtcg aggtcggcaa aacgagcacc
      481 gggcagatgc cctgcccggt aggccgcaat ccctggcggg ccggccaggc tccaccggac
      541 gtcgatgacg accggcggac gatcactggc gatttcggcc gcgagcgagg tggcatcgac
      601 gaggagatca gcgcgagacg gcatcggccc agtctgccgc acgcagccct agcggacgac
      661 gcggtatttg atgtgcgtga cgcccgggga cgcgaccacg ttcgcgggtt cgaagacaac
      721 atctccgaca tcgtcgaaga tccgctcgcc ggcgccgagg acgatcggca ctatgtgcag
      781 gtagagctcg tcgagcaatc ccgcggcgag gtattggcgc acgacactcc caccgccggc
      841 gatcgcaacg tccttgccat cagcagaagc gcgcgcttgc ttcaacgccg actcgacgcc
      901 gtccctcacg aagaagaacg tcgtgtcgtg caacgagagc ggagctcgct cgtggtgggt
      961 cagaacgaac acgtccttgt gaaatggcgg atcgtcgccc caccagccgg tccacgtttc
     1021 gtcccacggc ccgtcaccgc cgccgaacat cttgcggccc atgatgtggg cgccgattcc
     1081 cgccattgcc tcagaaataa cgtccgaatc ggccgacggg gacccgccgt ccaagccctg
     1141 tagctcgcgc caactggcgg tcccgaacac ccactcgtgc aggcgcatgc cgccttcgcc
     1201 gatcggattc tccaggctct ggttcggccc ggccacgaaa ccgtcgaccg atatcgagat
     1261 ctggcacttg actgaactca tgccatgttg acgcatgcca gactgagaac tcatcgcccg
     1321 atccctggag tcgtgacctc gttcaccgtg ttagcagcga gcgctgcagt aacgcgcgca
     1381 cgcaacaaaa ggtactgctc ggcacctcgc gctggcagtg cagttgtgcg gacgacgacc
     1441 ttcggctccc gaacggcctc agacgtgtgg aactgctcgc cagtcagatc gacctcgatg
     1501 tcgtcggcca accggttcca gtagtggtag ccctgtaggg agccatccgc gttccgcacc
     1561 tcggcaagaa gcaacacgcc gccgagcaga tcatgcagca ccagagccgt agaaccgcac
     1621 tgcccccgag ccggattgtc gggagaccaa tcggcgacgt cgactgggtc acacgtgtct
     1681 gccgcccacg cgtcgcgaac cgcggcctcg acttcgcgca aagcgatcgg tgtcacgggt
     1741 cgaatcgtgg cacacagcac ggacagcgct tgcgcggcgg taccttgcat gtcgtcaggt
     1801 cgtctattcg gtggagtcgc attgccagtg tttcgtcgca cggcatcgtc gacgggcgag
     1861 ccaatctcgg atttttggcg ttggtggaac aacgagggat ccggcaagct cgccgcggcg
     1921 attcgccgag gcggcgactt cggaaggctg cctggccgag tatccgaact cgtgagcgcg
     1981 atcaatcctg aacttggctg ggagctcgga ccaggccaca aggcccagca cgtcctatgc
     2041 gttacggcag gcggagtcgc ggctgtacgg ccgaccgccg agcgttggtt acgcgcagcc
     2101 cctccggtcg attcagcgtg ggagtacatc gctgcaaagg cggcgcaccc gctcccgctc
     2161 gacgacaaac ttgtacttgg tacgtggacg ctcaatattg ctgaaacacg gttcgggatt
     2221 gtggtcgagg agagccggct ggaagtggac gtgacctgct tccatccgca cttccccgtg
     2281 atgcccgagg acgcccgcaa tgaggtcatg tttatcgttc tcgattggct tctcggcgag
     2341 gatgacatcg aacgctgggt cggccacctc
//
LOCUS       DASWVF010000018         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_280842, whole genome shotgun sequence.
ACCESSION   DASWVF010000018 DASWVF010000000
VERSION     DASWVF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280842"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VGD55_00315"
     CDS             <1..711
                     /locus_tag="VGD55_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY0868817.1"
                     /translation="KHWKKELVEAPRSDRRRIRTSRVINFTGAVATAVVLGIVLYAKV
                     PDGAWIAIVAMVLLFAVMKGIRRHYTAVSKELDIEAAETPTTMPSRNHAVVLVSRLHL
                     PALRALAYAKATRPSTIEAVTINADDEEARSLQEQWQARGIDVPLVLLDSPYREVTRP
                     LLEYIRGRRGQSPRDVVTVFIPEYVVGHWWEQLLHNQSALRLKARLLFEPGVMVTSVP
                     WQLHSTDRIREASTNTPE"
     gene            766..1737
                     /locus_tag="VGD55_00320"
     CDS             766..1737
                     /locus_tag="VGD55_00320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0868818.1"
                     /translation="MEYTTLGRTGLQVSRIALGCMSYGEPERGGWPWSLGEEASLPFF
                     KQALDAGINFFDTANIYSAGSSEEITGRALSKFAPREDLVIATKVNGAMRPGPNGGGL
                     SRKAILTEIDNSLRRLGTDYVDLYQIHRWDYHVPIEETLEALHDVVRAGKARYIGASS
                     MHAWQFTKALYLADLHGWTRFVSMQNHYNLLYREEEREMIPLCIDQGVGIIPWSPLAR
                     GKLARQWDDTTTRSESDEFGRTLYHADDRIVVDAVGQVADQRGITRAQVAIAWALAKP
                     GITAPIVGATKPHHLADAIAAVEIHLSADEIESLEKPYQPHDVAGFS"
     gene            1739..2401
                     /locus_tag="VGD55_00325"
     CDS             1739..2401
                     /locus_tag="VGD55_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0868819.1"
                     /translation="MTAGVDVPDRRGRVGLDQVGRDLRRNPRVEVRDVEVTSDGWHVL
                     RRTTFDYQSSDGGWSTQHRETYDRGNGATILLYDAERRTVLLSRQFRYPVYVNGHPDG
                     MLIETAAGLLDEDDPETAIRREASEELGVTVGELTHVFDVYMSPGSVTERVHFFAAPY
                     SDASRTSDGGGLVDDGEDIDVLEVPFDDALAMVGTGEIADAKTIMLLQWAALNGPFRR
                     TT"
     gene            complement(2409..3377)
                     /locus_tag="VGD55_00330"
     CDS             complement(2409..3377)
                     /locus_tag="VGD55_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEY0868820.1"
                     /translation="MLAGTEHFLSDSPGGVRHVVQFYGPDDDLLDTVPDFLLDALHVD
                     GLAIAVATAPHCLAFEARIAAAGVDVAVAKRSGHLVTLDADETLQEFLVDGRPDWAGF
                     ESILGGIIRQATASGWPVRIFGEMVSLLWDSGHISAAVEVEMFWDELCRQLRFGLFCA
                     YRINELAGDDESEAFAEICSLHSSVVGAPGARPVLHTTKTFAKGVDAPRAARRFVITT
                     LVKWGDRAFVDDAALVVTELATNAVVHADSDFTVAVSSSPTTVRISVGDASSVAPKLR
                     DASLIAASGRGLGMVASVVSHWGDEPFGVGKVVWAEFQRPASADAD"
     gene            complement(3503..3578)
                     /locus_tag="VGD55_00335"
     tRNA            complement(3503..3578)
                     /locus_tag="VGD55_00335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3543..3545),aa:Arg,seq:acg)
BASE COUNT          713 a         1225 c         1201 g          570 t
ORIGIN      
        1 aagcactgga agaaagagct ggttgaagcg ccacgcagcg accgccggag gatccgcaca
       61 tcgagggtca tcaacttcac cggcgcggta gcgactgcgg tggtgctcgg catcgtgctt
      121 tacgcaaagg tcccggacgg cgcgtggatc gcgatcgtcg ccatggtgct gctcttcgcc
      181 gtcatgaagg gcatcaggcg ccactacacg gcggtgagca aggagctcga catcgaggcc
      241 gcagagaccc cgaccaccat gccgtcacgc aaccatgcgg tggttctcgt gtcgcgcctg
      301 cacttgcctg cgcttcgggc gctcgcctac gcgaaggcga cacgtccgag caccatcgag
      361 gccgtcacca tcaacgctga cgacgaagag gcgaggtcgt tgcaggagca atggcaggcg
      421 cgcggcatcg acgtcccgct cgtcttgctt gattcgccct accgtgaggt gacccgaccg
      481 ttgctcgaat acattcgcgg acgccgaggg cagagccccc gcgacgtcgt gaccgtgttc
      541 atccccgaat acgtcgtcgg gcattggtgg gagcagttgc tgcacaacca aagcgcactg
      601 cgactgaagg cgcggctgct gttcgagcca ggcgtcatgg tgaccagcgt gccgtggcaa
      661 ctgcactcga ccgaccggat ccgagaggcc tcgaccaata cgcctgagtg agcggcgcgg
      721 tctcgggtta ggctcgaaat ctcacgcacg tcgaggaggc ttccgatgga gtacacgacg
      781 ctgggtcgca ccggcctaca ggtatcgcgc atcgcgctgg gttgtatgag ctacggcgag
      841 ccggagcgag ggggttggcc gtggtcgctc ggcgaagaag ccagcctgcc gttcttcaag
      901 caggcactcg atgctggcat taacttcttc gacaccgcga acatctactc cgcgggcagc
      961 agcgaagaaa tcaccggccg ggcgctgtcg aagttcgcgc cccgtgaaga cctcgtgatc
     1021 gccaccaagg tcaacggcgc gatgcggccg gggccgaatg gcggaggcct gtcgcgcaag
     1081 gcgatcctga ccgagatcga caacagcttg cgccggcttg gcaccgacta cgtcgacctc
     1141 taccagatcc accggtggga ctaccacgtt ccgatcgaag agaccctcga ggcgctccac
     1201 gacgtggtgc gggccgggaa ggctcgttac atcggcgcct cgtcgatgca cgcgtggcag
     1261 ttcacgaagg ccctctacct ggccgacctg cacggctgga ccagattcgt cagcatgcag
     1321 aaccactaca acctgctcta tcgcgaggaa gagcgcgaga tgatcccgct ctgcatcgac
     1381 cagggcgtcg ggatcatccc ctggagcccg ctcgccaggg gcaagcttgc ccggcaatgg
     1441 gacgacacga ccacccgatc cgagagcgac gagttcggcc gcaccctgta ccacgccgac
     1501 gaccggatcg tcgtcgacgc cgtgggccag gtcgccgatc agcgcggcat cacgcgagct
     1561 caggtcgcca tcgcgtgggc gctagcgaag ccaggaatca cggcgccgat cgtcggggcc
     1621 accaagccgc accacctcgc ggatgccatc gcggcggtcg agattcactt aagcgccgac
     1681 gagatcgagt cgttggagaa gccctaccag ccgcacgacg tcgctgggtt cagctgaggt
     1741 gacggcgggc gtcgacgtcc ccgaccggcg cggccgggtc gggctcgacc aggtcggtcg
     1801 tgacctgcgg cgcaatcccc gtgtcgaggt gcgcgacgtc gaggtgacgt ccgacgggtg
     1861 gcacgtgctg cgccgcacga cgttcgacta ccagagctcg gatggcggat ggtcgacgca
     1921 gcaccgcgag acctacgacc gcggcaacgg cgcaacgatc ctgctgtacg acgccgagcg
     1981 gcgcacggtg ctgctgtcgc ggcagttccg ctacccggtc tacgtgaacg ggcaccccga
     2041 cgggatgctg atcgagaccg cagccgggct cctcgatgag gacgatcccg agaccgcgat
     2101 ccggcgcgag gcaagcgaag aactcggggt caccgtcggc gagctcactc acgtcttcga
     2161 cgtctacatg agtcccggat cagtgaccga gcgggtgcat ttcttcgccg cgccctacag
     2221 cgacgcgagc cgcacaagcg atggcggtgg gctcgtcgac gacggtgaag acatcgacgt
     2281 gctggaagtg cccttcgacg acgcgcttgc gatggtcggc accggcgaga tcgccgacgc
     2341 caagaccatc atgctgttgc agtgggccgc tttgaacggg cccttccgcc gcacgacgta
     2401 gaccaagctc agtcggcgtc cgccgaggca ggtcgctgga actcggccca tacgaccttg
     2461 ccgacgccga agggttcgtc gccccagtgg ctgaccaccg aggcgaccat gccgaggccg
     2521 cgacctgacg ccgcgatcaa agacgcgtcg cgaagcttcg gagccaccga actggcgtcg
     2581 ccgacggaga tgcggacggt agtcggcgac gacgagactg cgacggtgaa gtcagaatca
     2641 gcgtgcacga cggcgtttgt cgcgagttcg gtaaccacca gcgcagcgtc atcgacgaaa
     2701 gcgcggtcgc cccacttcac cagcgtggtg ataacgaatc gtcgcgccgc acggggagca
     2761 tcgaccccct tcgcaaaggt ctttgtcgtg tgcaacaccg gccgcgcgcc cggggcgccg
     2821 acaacactcg agtgcaggct gcaaatctcg gcaaacgcct cgctttcgtc gtcgccagct
     2881 aactcgttga ttcggtaagc acaaaaaagc ccgaaccgca gttgccggca aagctcatcc
     2941 cagaacatct cgacttcgac ggcagcgctg atgtgtccgg agtcccacag cagcgacacc
     3001 atctcgccga agatccgcac cggccacccg ctggccgttg cctgccgaat gatgccaccg
     3061 aggatcgact cgaagcctgc ccaatcgggc cggccgtcga ccagaaactc ctggagggtc
     3121 tcgtccgcat caagtgtcac gaggtgcccg ctgcgcttgg ctaccgcgac gtcgacgcca
     3181 gctgcggcaa tgcgagcctc gaaggcgagg cagtggggcg ccgtcgcgac tgcgatggcc
     3241 agaccgtcga cgtgaagagc gtcgagcagg aaatccggaa cggtgtcgag caagtcgtcg
     3301 tcaggcccgt aaaactggac gacatgacgc acgccaccgg ggctgtcgga caggaagtgc
     3361 tcggtcccag ccagcatcgt ttactccatg gaaggctggg agcgcttacc ccaacgacct
     3421 gcgaatatgc ttgtacacga ggctactctc gctcggcacc gcgagacccg cggcccgtcc
     3481 gcccaacggg gtcaagctca cgtggtgcgc tcggagggat tcgaaccccc aacctcttga
     3541 tccgtagtca agtgctctat ccgttgagct acgagcgcct gcgtcgcgca ccaagggcgc
     3601 acgtgtccga gcaagggtac agcgtgccga tcaccgctcc tcaggtctga caactcacgg
     3661 cggaggctcg gggatttgaa ccccggatgg gctttaagac ccaaaccgc
//
LOCUS       DASWVF010000019         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_26124, whole genome shotgun sequence.
ACCESSION   DASWVF010000019 DASWVF010000000
VERSION     DASWVF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26124"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1756
                     /locus_tag="VGD55_00340"
     CDS             <1..>1756
                     /locus_tag="VGD55_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868821.1"
                     /translation="NWALADGVWSRSQGNPFFAEELIAAREDASLSPELQGVILSRVE
                     ALSKPTQQLLRAIAAAGTTVTDRLVAALHVLDSDDLDLALSEAVDKQVLVVDPVIGGY
                     RFRHALLREAVYAAMLPGEARRLHRVLATTLTADPSLGASGPGYRIAELAGHWWAAGD
                     WPQAVESSFAAAEAAEAVWAFPEALAHFEHALTALDRMPGGAPVERVQLLAKVSAVAY
                     LADAGERALELARAAIDASGGDDDPFRVAHYYTLLGRHAWAMGLSDAAFDAYRTAAAM
                     LPTDPPSVELAEIIAEEARWLMLVARMSESEVLCHEAIAVARAVDARAVEGHALNTLG
                     VCRADLGHPDEGLELLRAALAIAVEVGSAFDIDRAYINLGHVLTDWAELEAAVDLVAE
                     CRAASEQRGFANLNGAVINGVWALFRLARYDDAEAMLASWGRRGVGTSDIAPQLVPAI
                     IAIRRGQLDEAEGLLAEAERSSVNLRDIQTRGRLYTENAELAIERGLPDEAYAYVERA
                     LALVTVTDDAARRAHMCALGVHALADRLDNDRAQGRHADVETAQRVAAGLVQEANRPV
                     DNGAGLRAPQAMACIAMCV"
BASE COUNT          281 a          546 c          603 g          326 t
ORIGIN      
        1 aactgggcgc tcgccgacgg cgtctggtcg cgttcgcaag gaaatccttt cttcgccgaa
       61 gaactcattg cggcgcgaga agatgcgtcg ctgtcgccag agttgcaagg cgttatcctc
      121 agtcgcgtcg aagcgctctc gaagcccaca cagcaactgc ttcgcgccat tgcagcagcc
      181 gggaccacgg ttaccgaccg tctggtggca gcgctgcacg tcctcgactc ggatgacctt
      241 gaccttgccc tctccgaggc ggtcgacaag caggtcctcg tcgtcgatcc tgtcatcggc
      301 gggtatcggt ttcgccacgc gttgttgcgc gaggccgtgt atgcggcgat gctgccaggc
      361 gaggcgcgtc ggttacatcg agtcttggca acgacgctca ccgccgatcc atcgctcggg
      421 gcgtccggac cgggatatcg gatcgccgaa ctcgccggtc attggtgggc ggccggtgat
      481 tggccgcaag ccgtcgagtc atcgttcgcc gctgccgagg cagccgaggc ggtgtgggca
      541 ttccccgaag cgctcgctca tttcgaacac gcgttgacgg cactcgaccg aatgcccggc
      601 ggtgcaccgg tcgagcgagt gcagttgctc gcgaaggttt ctgccgttgc gtatctcgcg
      661 gacgccggcg agcgtgcgct tgagttggca cgcgcggcca tcgatgcgtc cggtggagac
      721 gacgatccgt ttcgcgttgc gcattactac accctgctcg gccggcacgc ttgggcgatg
      781 ggcctatcgg acgcagcgtt cgacgcctac cgaacggcgg cggcgatgct ccccaccgac
      841 ccgccttcgg tcgagctcgc cgaaatcatc gcagaagagg cgcggtggtt gatgctcgtc
      901 gcgcgaatga gcgagtctga ggttttgtgt catgaggcga tcgccgtcgc gagggcagtc
      961 gatgctcggg cggttgaggg acacgctctc aatacgttgg gtgtctgtcg tgccgatctc
     1021 ggtcaccccg acgaaggtct cgaattattg cgcgctgcgc tcgccatcgc tgtcgaagtt
     1081 gggagcgcgt ttgacatcga tcgggcgtac atcaacctcg gccacgtgct caccgattgg
     1141 gccgaactcg aggccgccgt cgacctggta gccgagtgcc gtgcggccag cgagcaacgc
     1201 ggctttgcca atctcaacgg ggcggtcatc aacggcgtgt gggcactttt tcggctggcc
     1261 cgatacgacg acgccgaggc gatgcttgcg agctgggggc gtcgaggagt gggcacgtcg
     1321 gatatcgccc cgcagttggt gccggcgatt atcgctatcc gtcgcggtca gctcgacgag
     1381 gcggaggggc tgctagctga ggctgagcgg tcatcggtca acctccgcga tattcagacc
     1441 cggggccgcc tctacaccga gaatgccgag ttggccatcg agcgcggcct gcccgacgag
     1501 gcgtatgcct acgtcgaacg tgcactcgcg ctcgtcacgg tcaccgacga cgcagctcgt
     1561 cgcgcacaca tgtgtgcgct gggggtgcac gcactggcgg atcggctgga caacgaccga
     1621 gcccaggggc gacatgccga cgtcgaaacg gcgcaacggg tcgcggctgg gttggtgcag
     1681 gaagcaaacc gacccgttga caatggcgcg ggcttacgtg cgccgcaggc gatggcgtgc
     1741 atagcgatgt gcgtcg
//
LOCUS       DASWVF010000020         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_178997, whole genome shotgun sequence.
ACCESSION   DASWVF010000020 DASWVF010000000
VERSION     DASWVF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178997"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VGD55_00345"
     CDS             <1..644
                     /locus_tag="VGD55_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEY0868822.1"
                     /translation="TADVVDNEGNPVPLGGAGYLVITQPWPAMLRTLFNDPDRYVETY
                     WRRFSKPEDDRWIYFPADGCKRDAEGYYWLLGRVDDVMNVSGHRISTLEVESALVDHP
                     SVAEAAVVGRNDARTGQAIAAFVSPKSGVVADEALIQELRDHVAKKIGKIARPSSIVF
                     TDELPKTRSGKIMRRLLRDVSEQRQLGDVTSLANADVVRDIAERAKTMVTQEE"
     gene            706..1344
                     /locus_tag="VGD55_00350"
     CDS             706..1344
                     /locus_tag="VGD55_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEY0868823.1"
                     /translation="MSSQTPVAPAPVLGIADPGPLGLAGFAMTTFVLSTFNTNVLNPS
                     MEAAVLPLALFYGGLVQLLAGMWEFRKGNTFGATAFASFGAFWLAFYWLVTYDAAHLA
                     PADQAHKAVGLFLLSWAIFTVYMTVAAMRTSGAVLAVFVALSITFILLAIGNFATGNT
                     SKDWIHIGGWFGYLTALLAWYASFAGVTNATFAKIVLPTFPLTGRPAAVTRA"
     gene            complement(1369..>1675)
                     /locus_tag="VGD55_00355"
     CDS             complement(1369..>1675)
                     /locus_tag="VGD55_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEY0868824.1"
                     /translation="QLVRISVTADRAKTKNLLRDPRANLHVMTPDGWSWMVAEGTATL
                     TPVAQDPHDATVEELIVQYRGVSGEHPDWDDYRRAMVEDRRLVVRLELQRTYGSAAR"
BASE COUNT          288 a          557 c          512 g          318 t
ORIGIN      
        1 tcactgcgga tgtcgtcgac aacgaaggca atccagtgcc gctcggtggt gcgggctacc
       61 tcgtcattac acaaccctgg cccgcgatgt tgcgcacgct gttcaacgat cccgatcgct
      121 acgtcgaaac gtactggcga cgtttcagca aaccggaaga cgaccgctgg atctatttcc
      181 cggccgacgg ttgcaagcgc gacgcggagg gttactactg gctgctcggt cgggtcgatg
      241 acgtcatgaa tgtctcgggt caccgcatct cgaccctcga ggtcgagtcc gcactcgtcg
      301 atcacccgtc ggtcgcagaa gcagcggtcg tcgggcgcaa cgacgcccgc accgggcagg
      361 ccatcgcggc attcgtctca ccgaagagcg gcgtcgtggc cgacgaggcg ttgatccaag
      421 aattgcgcga ccacgtcgcg aagaagatcg gcaagatcgc gcggccgtcg tcgatcgtgt
      481 tcaccgacga gctgccgaag acacgcagcg gcaagatcat gcgccggctg ttgcgcgacg
      541 tttccgagca gcggcaactc ggtgacgtga cttcactcgc caacgccgac gtcgtccgcg
      601 atatcgctga acgagcgaaa acgatggtca cccaagagga gtaagccgca ccacagcgca
      661 cgtccccgat gtcaaccagg acccagttcc catccaggag gcacagtgtc cagtcagacc
      721 ccggtcgcac ccgcaccggt actaggcatc gccgacccag gcccactcgg cctcgccggc
      781 tttgcaatga ccacgttcgt gctgagtacg ttcaacacga atgttctcaa cccgagcatg
      841 gaagcggccg ttctaccact cgcgcttttc tacggcggcc tcgttcagct gctcgcgggc
      901 atgtgggaat tccgcaaggg caacacgttc ggcgccacgg cgttcgcgtc gttcggcgca
      961 ttctggttgg cgttctactg gttggtcacc tacgacgccg cgcatctggc ccctgccgac
     1021 caagctcaca aggcggtcgg tctcttcctg ctgtcgtggg cgatcttcac cgtctacatg
     1081 acggtcgcgg cgatgcgcac gagtggcgca gtgctcgcgg tgttcgtcgc actgtccatc
     1141 accttcatct tgctcgccat cggcaacttc gccacgggca acaccagcaa ggactggatc
     1201 cacatcggcg gctggttcgg ctacctcacc gcactgctcg cgtggtacgc ctcgttcgca
     1261 ggggtcacca acgcgacgtt cgccaagatc gtcctaccga ccttcccgtt gacgggtcgt
     1321 cctgcggccg ttacgcgcgc ctagcaggtt tgtcgacgca cctacggttc agcgcgctgc
     1381 tgagccgtag gtgcgttgca attctaggcg gacgacgagg cgccggtctt ctaccatcgc
     1441 tcggcggtag tcatcccaat cgggatgctc gcccgacacg ccgcggtact ggacgatcag
     1501 ctcctcgacg gtcgcgtcgt gcgggtcttg tgccacgggg gtgagggtcg cggtgccttc
     1561 ggcgaccatc cagctccagc cgtcgggtgt cataacgtgc aggttcgccc tcggatcgcg
     1621 cagcaggttc ttggtcttgg cgcggtcggc cgtgaccgag atgcggacca gttgc
//
LOCUS       DASWVF010000021         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_280912, whole genome shotgun sequence.
ACCESSION   DASWVF010000021 DASWVF010000000
VERSION     DASWVF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280912"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGD55_00360"
     CDS             complement(<1..576)
                     /locus_tag="VGD55_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0868825.1"
                     /translation="MIHLLIVDDHPAYRRGLGLMLADARDIEIVGEADTGVRAVELAT
                     SLTPDVVLMDLRMPDLDGIEATRRLRQSGSRAAVVVLTMFEDDDSVFAAMRAGATGYL
                     LKGAEQEEIERAIRAAAAGEAIFGPKIAQRVVAHFASGAGSSAGAFPALTEREREVLE
                     MVAAGKGNATIAHELMITLKTVRNHVSNIFTK"
     gene            complement(573..>2114)
                     /locus_tag="VGD55_00365"
     CDS             complement(573..>2114)
                     /locus_tag="VGD55_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0868826.1"
                     /translation="VGAAGDRDGFAKSHHGAGLPVAWLPASISGIFGGVGLLLVVLLF
                     FGSFVAVWRRLRRARGDERLQLLWLVWGALTVPVALLTGWANYFLAGDGPFFTPALGL
                     IAVVLPVTIGIAILRHRLFDIRLVLSRTLTYLLLIIAIVAVYALLLFVTGRFFSNRTL
                     GGLLAVGVVAIAVQPAYSSFRRRSERWVYGLRSDPRAAIRMMSDRAEAADPAGLTAAV
                     TEAVAVALKVNRVWVELSEPRPDEALRTRMVHRGELVGYLAVEVPPGRDFSQPDMDLL
                     VDLAGYVGILVRSEQLNDELRDSRSRIVAGREEERRRLRRDLHDGLGPSLAATVLKLN
                     AAQSRTNSVERDSLLAETRDEVKEAITEVRRLVDDLRPPAIDEVGLLDAIRQRASALS
                     GVVTFEVTGTDRLADPRTQLPAAVEVAAFRIASEAMTNVVRHSGATRCLVRVELNGAF
                     QLTVSDNGRGTDRTVSTGVGWTSMRERASELGGTCTISSEPAGGLTVRAILPLVHGEP
                     VNADT"
BASE COUNT          394 a          690 c          702 g          328 t
ORIGIN      
        1 cttcgtgaag atgttcgaca cgtgattgcg caccgtcttc agggtgatca tcagctcgtg
       61 agcgatggtt gcgttgccct ttccggcggc gaccatctcg aggacctctc gttcgcgctc
      121 ggtgagagcg gggaacgcgc cggcgcttga gccggcgccg ctggcgaagt gcgcgacgac
      181 gcgctgcgcg atctttggtc cgaaaatcgc ctcaccggca gcggccgcgc ggatcgcccg
      241 ctcgatctct tcctgttcag cccctttgag caggtagccg gttgctcccg cacgcatcgc
      301 ggcgaacacc gagtcgtcgt cctcgaacat ggtgagcacg acgactgcgg ccctggatcc
      361 ggactggcgc agtctgcggg tcgcctcgat gccgtcgagg tcgggcatgc gaaggtccat
      421 cagcaccacg tccggggtga gcgacgtcgc gagttcgacg gctcgaaccc ccgtgtcggc
      481 ttcgccgacg atctcgatgt cacgagcatc agccagcatc aatcccagcc cgcgtcggta
      541 cgccgggtga tcgtcgacga tcagcagatg gatcatgtgt cggcgttcac cggctcgccg
      601 tgaacgagcg gcaggatcgc gcgaacggtg aggccgccgg ccggctcgct gctgatggtg
      661 caggtgccac cgagctcgga ggctcgttcg cgcatcgacg tccagccaac cccggtcgag
      721 acggtccgat cggtgccgcg tccgttgtct gagaccgtca actggaacgc gccgttcagc
      781 tcgactctga ccaggcaccg ggtggcgccg gagtgccgga cgacgttggt catcgcctcg
      841 gaggcaatcc gaaaggcagc aacctcgacc gctgctggca actgagtgcg tggatcggcg
      901 agacggtcgg tgcctgtcac ctcgaacgta acgacacccg acagcgccga cgcgcgttgc
      961 cggatcgcat cgaggagtcc gacctcgtcg attgctggtg gacgcagatc gtcaacgagc
     1021 cgacgcacct cagtgatggc ttccttgacc tcgtcgcgcg tctcggcgag gagtgaatca
     1081 cgttcaacgg agttcgtccg ggactgcgct gcattgagct tgagaacggt agcggcgagt
     1141 gaaggaccga gaccatcgtg caggtcgcgc cgcagccggc gccgctcctc ctcacgccca
     1201 gcgacgattc tgctgcgcga gtcgcgtagc tcgtcgttca gctgctcgga ccgcaccagg
     1261 atccccacgt agccagccag atcaacgagc aagtccatat ctggctggga aaagtcgcgc
     1321 cctggcggaa cctcgacggc gagatacccg acgagttcgc cgcgatggac catgcgcgtg
     1381 cgcagtgcct cgtcgggcct gggttcggac agctcaaccc agacacgatt caccttgagc
     1441 gcgacggcga cggcctcggt aacggccgcg gtcagcccgg ctgggtcggc agcttcggcg
     1501 cggtcggaca tcatccgaat cgccgcgcga ggatcggagc gcaggccgta gacccaacgt
     1561 tcgcttcgac gtcgaaatga cgaatatgca ggctggacgg cgattgcaac gacaccgacg
     1621 gccagcaggc cgcccagcgt gcggttactg aagaagcgtc cggtcacgaa aagcagcaac
     1681 gcgtagacgg cgacgatggc gatgatgagg agcagatagg tcagcgtgcg tgagagcacg
     1741 agccggatgt cgaagagccg gtgccgcaag atggcgatgc cgatggtgac gggcagaacg
     1801 accgcgatca gacccaacgc cggagtgaag aacggtccgt ctcccgccaa gaaatagttc
     1861 gcccagccgg tgagtagtgc gaccggaacc gtgagcgccc cccacaccag ccagagcagt
     1921 tggagccttt cgtctccgcg tgctcggcgc agccgtcgcc agaccgcgac gaagctgccg
     1981 aagaagagga gcacgaccag caggagaccg acaccgccga agatcccaga gatggacgcc
     2041 ggcaaccacg cgaccggcag acccgcgccg tgatggctct tcgcgaaccc gtcacggtcg
     2101 ccagctgcgc cgac
//
LOCUS       DASWVF010000022         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_382884, whole genome shotgun sequence.
ACCESSION   DASWVF010000022 DASWVF010000000
VERSION     DASWVF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382884"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VGD55_00370"
     CDS             complement(<1..771)
                     /locus_tag="VGD55_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0868827.1"
                     /translation="MTAVQERPPYVVLRHSRQSRVSSIVIVVVAIVLVTLPTWQSANI
                     IQQTTSLLITAILAVMWNALAGYGGMVSVGQQAFIGLGAYGTIYFTQHGVTPYLAMVL
                     AAIAAGVIGVPVSMLVLRLRGGQFAVGTWVVAEAFGLLVIIDKSVGGGTGTSLHGLNR
                     YGPLNREKYTYWLTLFFFLLACSLVFALLRSRHGVALQAIRDDEIAAAAVGVRVQASK
                     RVLFVLAAFGCGAAGALILANSLFVEPQSIFGVQYSAYM"
     gene            complement(768..1691)
                     /locus_tag="VGD55_00375"
     CDS             complement(768..1691)
                     /locus_tag="VGD55_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0868828.1"
                     /translation="MTWVNVVVQGVLLGGFYALLACGLSLMFGVMRIINLAHGDLAVL
                     GAFLVWETTKTFHISPFLAAVPVLVLMLGVGYLFYYGLLKRSVQAGPLVPLLTTFGVA
                     IIIQNAQLQIFSPDAKSLGSAAGKVATRSWRIHSHLAVSWLGMLTLAVAIATLGGLQL
                     FLQRTGPGREMRATALDADTAELVGINAHAVYARATAIALATATIAGTFLVIRSTIAP
                     SSGSSQLIFAFEAVVIGGLGTLWGTLWGGVVLGVAQAVGAQIHPQYSILAGHLVFLVV
                     LATPQVRARLFRGARIFKPGSTGRALGRVPA"
     gene            complement(1688..>2110)
                     /locus_tag="VGD55_00380"
     CDS             complement(1688..>2110)
                     /locus_tag="VGD55_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868829.1"
                     /translation="GRHARPGPWNLDTVLDAFTLLRPLQNKTASTLSGGEQQATAIGR
                     ALMTNPRVLLMDEVSLGLAPIAVAAVYESMGSLIESGTAIILVEQDLSRAMSVVDRLI
                     CMLEGAVVLEGSAAELTREQITEAYFGLKTFRDKGVRR"
BASE COUNT          443 a          694 c          673 g          300 t
ORIGIN      
        1 catgtaggcg ctgtattgaa cgccgaagat cgactgcggc tcgacgaaca gcgagttcgc
       61 caagatgaga gcgccggccg cgccgcaccc aaaagctgcg agtacgaaca agacacgctt
      121 acttgcctga acccggacgc cgacagcagc cgcggcgatc tcgtcgtcgc gaatcgcctg
      181 caaagccacg ccgtggcgac tgcgtagtag cgcgaacacg aggctgcaag ccagcaggaa
      241 gaagaacagc gtcagccagt acgtgtactt ctcacggttc aagggcccgt aacggttgag
      301 gccgtgcagc gaggttccgg taccaccgcc gacgcttttg tcgatgatga ccagcagacc
      361 gaacgcctcg gccaccaccc aggtgcccac cgcgaactgg ccaccgcgaa gccgcagcac
      421 gagcatcgac acaggcacgc cgatcacacc cgccgcgatc gctgcgagca ccatcgcgag
      481 atacggcgtg acaccgtgct gggtgaagta gatcgtgccg tacgcgccga gaccgatgaa
      541 ggcttgctga ccgaccgaga ccatgcctcc gtagccggcc agcgcgttcc acatcactgc
      601 aaggatcgcg gtgatcagca gcgacgtcgt ctgctgaatg atgttcgccg actgccacgt
      661 tggaagagtc acgagcacga tcgccaccac cacgatgacg atcgacgaca ccctcgactg
      721 gcggctgtgc ctgagcacga cgtagggggg ccgctcctgc accgcggtca tgcgggcacc
      781 cgtccgaggg cacgaccggt ggagccgggc ttgaagatgc gcgcgccacg aaacaaccgc
      841 gcacgcacct gcggcgtcgc gagcacgacg aggaacacca ggtggccggc aaggatcgag
      901 tattgcggat ggatctgcgc gcccacggcc tgcgcgacgc cgagcacgac cccgccccac
      961 aacgtgcccc acagcgtgcc caacccgccg atgacgacgg cttcgaacgc gaagatgagt
     1021 tggctagatc cactcgacgg cgcgatggtc gacctgatga ccaggaacgt gcccgcgatc
     1081 gtggcggtgg ctaatgcgat cgcggtggcg cgcgcgtaca ccgcgtgcgc gttgataccg
     1141 acgagctcag ccgtgtcggc atcgagtgct gtcgctctca tctcacggcc cgggcccgtg
     1201 cgttgcaaga agagctggag accgccaagt gtcgcgatcg ccacggccag cgtgagcatg
     1261 ccgagccacg agacggccag gtgggaatgg atgcgccaac tgcgggtcgc gaccttgccc
     1321 gcggccgacc cgagtgactt cgcgtcgggt gagaaaatct ggagctgcgc gttctggatg
     1381 atgatggcga caccgaaggt cgtcagcagt ggcaccagcg ggcccgcctg cacactgcgc
     1441 ttgagcagcc cgtaatagaa gaggtagccg acgcccagca tcagaacgag caccgggacc
     1501 gcagcgagga acggcgagat gtgaaacgtc ttcgtcgtct cccacaccag gaaggcgccg
     1561 agcacggcga gatcgccgtg ggcaaggttg atgatccgca tgacgccgaa catcaacgag
     1621 agaccgcagg cgagcaacgc gtaaaaccca ccgagaagca cgccctgtac gacgacgttg
     1681 acccaggtca tcggcgcacg cctttgtccc ggaacgtttt caacccgaag tacgcctcgg
     1741 tgatctgctc gcgagtcagc tccgcagcgg aaccctcaag aacgaccgcg ccctcgagca
     1801 tgcagatcaa ccgatcgacc acgctcatcg cacgggagag atcctgctcg acgaggatga
     1861 tcgccgtccc ggattcgatg agtgaaccca tcgattcata gaccgccgct accgctatgg
     1921 gagcgaggcc gagtgacacc tcgtccatca gcagcacgcg tgggttcgtc atgagcgcgc
     1981 ggccgatcgc agtcgcctgc tgctcacccc ctgacagcgt cgaggccgtc ttgttctgaa
     2041 ggggtcgcag cagggtgaac gcatcgagga ccgtgtcgag gttccatggt ccgggccgag
     2101 cgtgccggcc
//
LOCUS       DASWVF010000023         6601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_408369, whole genome shotgun sequence.
ACCESSION   DASWVF010000023 DASWVF010000000
VERSION     DASWVF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408369"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VGD55_00385"
     CDS             complement(<1..1376)
                     /locus_tag="VGD55_00385"
                     /inference="COORDINATES: protein motif:HMM:NF004561.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0868830.1"
                     /translation="MSPGVGREGAGGASVAGPNTVNLGPTDRDRVGGAGVRGYNGARA
                     RSRTVRRNDDASTSAETTGATTKARSNLNAATNPSTSATNGRAAAKNAGAGTAAAAKA
                     AKLVPVALVADPVDVVADVDPDVDADVFADDVVLEVDLVDVEVDIDVDIVADVIDPVD
                     PAAIVVAGEPVEEVEDDEEPTEVVVEDDGEPQAQLVSAGATADPVKDYLKQIGKVPLL
                     NAEQEVELAKRIEAGLFAEEKLNSGEKLTVKLRKEFEWIAEDGRRAKNHLLEANLRLV
                     VSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMAD
                     QARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAAELDMTPEKVIEVQKYGRE
                     PISLHTPLGEDGDSEFGDLIEDSEAIVPAEAVSFTLLQEQLHSVLDTLSEREAGVVSM
                     RFGLTDGQP"
     gene            complement(1395..2702)
                     /locus_tag="VGD55_00390"
     CDS             complement(1395..2702)
                     /locus_tag="VGD55_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868831.1"
                     /translation="MSRIVVDYEELRRMSSVWAEAAATMARLGFRVADLAVTAEIVSN
                     AVFDPAGAARCETAILDAAVGPHGLAMLAMKLAADAALLRAVVAKEQLVDDFPIRQLV
                     ALEASLVTLPLTAIRDPARATRVTAQRAVTLADASVGYVAPFTTPLLETFAPSARFRF
                     DAMMRRPVSADPVFGLPLGAALPASERDGGSVSISRYLPAWGGAPPSSNSAVLNEVGD
                     LENQPDASVAVQRVVGRDGVARYVVALSGMRHIVSTPDPEDLVGALGAVVGTHTNYTT
                     CVREALDTALVPIGAQVLLVGHSQGGIVAMDLAGDPGFNGERVRVVQVVAAGSPISSK
                     SVAPGSRTRVLSIENVNDIVTQLDAVDPPAGHPSIDRLVYRFAVDEHDVMANHDVRLY
                     SRQAEELSDSPNPLMIGVQAGLRPFLEGSTTTTVFTMHDRLVG"
     gene            complement(2699..3037)
                     /locus_tag="VGD55_00395"
     CDS             complement(2699..3037)
                     /locus_tag="VGD55_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868832.1"
                     /translation="MADIDGIAQELGQRAVQVEMAGAQLVQAAAAAVWTSIAADAFRA
                     QVDHRRRECERVAAVLRGAAHDVLHFSSDVTAEKARLRRLELAAEHAAAAALSGAARA
                     AHGLGSIVRL"
     gene            complement(3172..3744)
                     /locus_tag="VGD55_00400"
     CDS             complement(3172..3744)
                     /locus_tag="VGD55_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY0868833.1"
                     /translation="MLQLAQQPAADALLDSDPLALLVGMLLDQQIPMEKAFAGPHVLA
                     QRMGVKQLDAFAIADYDPEAFAKMFSTPPAIHRFPGSMAGRVQELARQLVSDYDGRAD
                     LVWSTAKSGADLHARLTALPGFGAQKARIFVALLGKQRGVRPTGWREAAGPYGEKDSY
                     RSVADVTSPESLQKVRAHKKEMKAAARAQA"
     gene            complement(3775..4398)
                     /locus_tag="VGD55_00405"
     CDS             complement(3775..4398)
                     /locus_tag="VGD55_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY0868834.1"
                     /translation="MDDHGLTLLDWRRRVHAVYAQVRAATDVAKAHAAWSAERDDLFA
                     HHPQSPIAVDRRATFIGVPVKPYDPAWRFELAVDTGVERVQIEIATGTDGIVAFDRIG
                     VVHIPDVGGLDVWALRSYGGGLFIPLRDRTANRTTYGGGRYLIDTAKGADLGSDATKG
                     TIVLDFNFAYHPSCAYDAAWACPLAPPGNVLDVEVPVGEILPDAGWY"
     gene            complement(4531..6531)
                     /locus_tag="VGD55_00410"
     CDS             complement(4531..6531)
                     /locus_tag="VGD55_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY0868835.1"
                     /translation="MPRSSPVRTRPVRIASSLAVAAALLGTFGAAPQAFATASASAKP
                     ASTKQPPAVIRVDQVGYLPTDAKHAYLMASHKVKHATVDVVSGGQVVAEAKLGTTNRG
                     PWNANYPDVYDITFSGVAPLGTYHLVAHGDASAQSPDFAIETPAALYGQVVEDGVNFY
                     QVQRDGADVVAGPLDRQPAHLGDAHATVYETPDFNDTTSDDSLVPGSPLVKINGAPKV
                     DVEGGWYDAGDYLKFTHTAAYGDVVLYASERALGNAAPSTLKTEAAYGESWLDKMWKM
                     STKTLYLQVGIGTGNGSDTVDPTFNGDHDLWRLPQADATDTDPLDRYATTQRPVFEAA
                     APGQLISPNLAGRVSAAFALAAQVDASTNKARAEYEYLEATSLYAMANTSNPPSPLVT
                     ADPEAYYPETTWHDDMELGATEIALAAQELSHDASPYLKAAAGFAKQYIADDAAAGDT
                     FNLYDTSALAHTDLVHAMAAAGNPKLAVSKADLIKDLKRQVELGASHASTDVFRAAGD
                     INQFDVDSHTFGWIAMEAWYTGLTGDHSFDAFAAEQRDWLFGANAWGTSFMVGEGSTF
                     PECMQHQVANLSGNLHGVGPALDVGAVVNGPNDNSNFEGGLGDLQDGMRECPTNGVDK
                     FAPFDTATASFVDDVRSWQTDEPALDMTGAAIIAAASQLALH"
BASE COUNT         1073 a         2182 c         2200 g         1146 t
ORIGIN      
        1 ggctgaccgt cggtcagacc gaaccgcatc gagaccacgc ctgcctcgcg ctctgacagc
       61 gtgtcgagca ccgagtgcag ctgttcctgc agcagcgtga acgacactgc ttcggccggc
      121 acgatcgctt cggagtcttc gatgagatcg ccgaactcgc tgtcgccgtc ctcaccaagg
      181 ggcgtatgca ggctgatcgg ttcgcggccg tacttctgca cctcgatgac cttttccggg
      241 gtcatgtcga gctcggcggc gagctcctcg ggggtgggtt cgcggccgag gtcttgcagc
      301 atctggcgct ggacgcgtgc gagcttgttg atgacctcga ccatgtgcac ggggatacgg
      361 atcgtgcggg cctggtcggc catcgcgcgg gtgattgcct gccggatcca ccacgtcgcg
      421 tacgtcgaga acttgtagcc cttggtgtag tcgaacttct cgaccgccct gatcaagccg
      481 aggttgccct cttggatcag gtcgaggaag agcatgccgc ggccggtgta acgcttggca
      541 agggagacga cgaggcgcag gttggcttcg agcaggtggt tcttggcgcg gcggccgtcc
      601 tcggcgatcc actcgaactc cttgcggagc ttgacggtca gcttctcacc ggagttgagc
      661 ttctcctcgg cgaacaggcc ggcctcgatg cgcttggcga gctcgacctc ctgctcggcg
      721 ttgagcagcg gcaccttgcc gatctgcttg aggtagtcct tgacggggtc ggcggtcgcg
      781 cccgcgctga caagttgcgc ctgaggctcg ccgtcgtctt cgacgacaac ctcggtcggc
      841 tcttcgtcgt cttcgacctc ttcgaccggc tcgcccgcaa cgacgatggc cgccgggtcg
      901 acgggatcga tgacgtcggc gacgatgtcg acgtcgatgt cgacctctac gtcgacaaga
      961 tcgacctcga ggaccacatc gtcggcgaag acgtccgcgt caacgtcggg gtcgacatcg
     1021 gcgaccacgt cgacgggatc agccaccagc gcgactggaa cgagcttggc agccttggca
     1081 gcggccgcgg tcccggcgcc agcgttcttc gcggctgccc ggccattggt cgcgcttgtg
     1141 gacgggttcg tcgcggcgtt caaattgctc ctggccttcg tggtagctcc cgtggtttcg
     1201 gcgctcgtac ttgcgtcatc gttccggcgg actgtgcgcg accgggctcg ggcaccattg
     1261 tagccacgga cccccgcgcc accgacccgg tcgcgatcag tcggccccag gttaaccgtg
     1321 ttcggcccgg ccacagacgc cccgcccgca ccttcacggc cgacgccggg gctcatcagg
     1381 cggctgactg ccgctcagcc cacgagccgg tcgtgcatcg tgaagaccgt tgtggtcgtc
     1441 gatccctcga gaaagggtcg caaaccggcc tggacgccga tcatgagcgg attcggcgaa
     1501 tcgctcagct cctccgcctg acgggagtac agacgcacgt cgtgatttgc catgacatcg
     1561 tgctcgtcga cagcgaaccg gtagacgagg cggtcgatgc tcggatggcc ggccggcggg
     1621 tcgacggcgt cgagttgggt cacgatgtcg ttcacgttct cgatgctgag gacacgggtg
     1681 cgactgcccg gcgccaccga tttggaactt atcggcgagc cggccgcgac gacctgcacc
     1741 acccgaaccc gctcaccgtt gaacccggga tcgccagcga ggtccatagc gacgatgcca
     1801 ccttgactgt gaccgacgag cagcacctgc gcgccgatcg gaacaagcgc ggtatcaagc
     1861 gcctcgcgca cgcaggtcgt gtaattcgtg tgagtgccta cgaccgcgcc gagtgcgcca
     1921 acgagatctt cggggtcggg agtgctgacg atgtgtcgca tgcccgacag agcaacgacg
     1981 tacctggcga cgccgtcgcg accaaccacg cgctgcactg cgaccgaggc atcaggttgg
     2041 ttctcaaggt cgccgacctc gttcaagacc gcggaattcg atgagggagg cgcacctccc
     2101 caggcaggga ggtatctcga tatcgagaca ctcccaccgt cacgctcgct cgcgggtagg
     2161 gccgcgccga gtgggagtcc gaacaccgga tcagccgata ccggccgccg catcatggcg
     2221 tcgaaccgga accgggccga cggcgcgaac gtctccagca gcggcgtcgt gaagggagcg
     2281 acgtagccga ccgaggcgtc ggcgagcgtg acggcccgct gagcggttac ccgggtagcc
     2341 cgggccggat cgcggatcgc tgtcaggggc agggtgacga gtgacgcctc gagagccacg
     2401 agctggcgaa tcgggaagtc gtcgacgagc tgctccttgg cgacgactgc gcgcaggagc
     2461 gcggcatcgg ccgccaactt catggccagc atcgcgagcc cgtggggtcc gaccgccgcg
     2521 tcgagaatcg cggtctcgca gcgcgcggcg cccgcggggt cgaacacggc attcgagacg
     2581 atctcggccg tcactgcgag atcggcaacc cggaagccga gccgcgccat tgttgctgcg
     2641 gcctccgccc agaccgacga catgcgccgg agctcctcgt aatcgacgac gatgcggctc
     2701 acaggcgcac gatcgagccg agcccgtgtg cagcccgtgc ggcccccgat agagcggcgg
     2761 cggcagcatg ctcggcagct agctcgagtc gccgcaggcg agccttctca gcagtgacat
     2821 cgcttgaaaa gtggagcaca tcgtgtgctg cgccacgcag cacggcggcc acgcgttcac
     2881 actcgcggcg ccggtgatcg acctgagccc gaaacgcatc ggctgcgatc gacgtccaga
     2941 ccgctgctgc cgctgcttga acgagttgag cacctgccat ctcgacctga accgcgcgct
     3001 gaccgagttc ttgcgcgatc ccgtcaatgt ccgccaccga cccgcccacc cgttcgtctg
     3061 tccgtccgca ccggcacgag ccggctggac gaacctacga tcgaaggagt cagaatcacc
     3121 gcatcgcgca acggacggtg gacataccgc gatggttgtg gaaactcaat gtcaagcttg
     3181 cgctcgcgcc gcggctttca tctctttctt gtgcgctcgg accttctgca acgattcggg
     3241 gctcgtgacg tcggcgaccg aacggtagct gtctttctca ccgtaaggtc cggcagcttc
     3301 acgccagccg gtgggccgca caccccgttg tttcccaagc agggcaacga aaatcctggc
     3361 tttctgcgca ccgaagcctg gaagtgccgt cagtcgcgca tgcaggtcgg cgcctgattt
     3421 cgctgtgctc cagacgagat cagcgcggcc gtcgtagtcg ctgacgagct ggcgcgcgag
     3481 ctcttgaacg cgccctgcca tcgagccggg gaaacggtga atcgccggcg gcgtcgagaa
     3541 catcttggca aaggcttctg ggtcgtagtc ggcgatcgcg aaggcatcga gctgcttgac
     3601 gcccatgcgt tgcgcgagca cgtgaggccc ggcgaaagcc ttctccatcg gaatctgctg
     3661 gtcgagaagc atgccgacca ggagcgcgag cggatcgctg tcgagcagcg catcggcggc
     3721 cggctgctgg gctagttgca acacagaaca gacgttacca agcccgcgcc cgggtcagta
     3781 ccacccggcg tccggcaaaa tctcaccgac cggcacctcg acgtcgagca cattgcccgg
     3841 cggcgcgagc gggcacgccc atgcagcgtc gtaggcgcac gaagggtggt aggcgaagtt
     3901 gaagtcgagg acgatcgtgc ccttggtcgc atcgctgccg agatcggcgc ccttcgcggt
     3961 gtcgatcagg tagcggccgc cgccgtacgt cgtgcggttc gccgtccgat cgcgcagcgg
     4021 gatgaacaag ccaccgccgt agctgcgcaa cgcccacacg tcgagccccc cgacgtccgg
     4081 aatatgcacg acaccgatcc gatcgaacgc aacgatgccg tcagtacccg tggcaatctc
     4141 gatctgcacc cgctcgacgc cagtgtcgac ggcgagctcg aaacgccacg ccgggtcgta
     4201 cggcttgacc gggacgccga tgaacgtggc acgacgatcg accgcgatcg gcgactgcgg
     4261 atggtgcgcg aacaagtcgt cgcgctcggc tgaccacgcc gcatgcgcct tcgcgacatc
     4321 ggtggccgcc cgaacctgtg cgtagacggc gtgtacccgc cgccgccaat ccagcaacgt
     4381 caacccgtga tcgtccatgg cgccagtctg ccgtccggcg cccgcgcggc gcgagcgacc
     4441 tgtgccttgt ttctggggcg ggccgcggtc gcgtgaacgc gatgccccgg gccgcgttca
     4501 cgtgaacgcg atgcccgcgc cgcgtgcggc tcagtgcagg gcgagttgcg acgcggccgc
     4561 gatgatcgca gcgcccgtca tgtcgagcgc cggctcgtcg gtctgccacg accggacgtc
     4621 gtcgacgaag ctcgccgtcg cggtgtcgaa cggcgcgaac ttgtcgacgc cgttggtcgg
     4681 gcactcgcgc atgccgtcct gcaagtcgcc gaggccgccc tcgaagttgg agttgtcgtt
     4741 gggtccgttg acgaccgcac ccacgtcgag agccggaccg acgccgtgca ggttgccgct
     4801 gaggttcgcc acctggtgct gcatgcactc cgggaacgtc gagccctcac ccaccatgaa
     4861 gctggtgccc cacgcgttcg cgccgaagag ccagtcgcgc tgttccgcag cgaacgcgtc
     4921 gaagctgtgg tcacccgtga gcccggtgta ccaagcctcc atggcgatcc agccgaacgt
     4981 gtgcgagtcg acgtcgaact ggttgatgtc gccggccgcc ctgaatacat ccgtcgacgc
     5041 atgcgacgca ccgagctcga cctgccgctt cagatctttg atcagatctg ccttgctgac
     5101 agcgagtttt ggattgccgg ctgctgccat cgcgtgcaca agatcggtgt gtgccagagc
     5161 actcgtgtcg tacaggttga aggtgtcgcc ggctgccgcg tcgtccgcta tgtattgctt
     5221 ggcaaaaccc gccgcggcct tcagatacgg cgaagcatcg tgcgacaact cctgcgcagc
     5281 aagcgcaatt tctgtcgcgc cgagctccat gtcgtcatgc cacgtcgtct cggggtagta
     5341 cgcctcaggg tcagcagtga caagagggga cggcgggttg ctggtgttcg ccattgcgta
     5401 cagcgaggtc gcctcaaggt attcgtactc ggcgcgggcc ttgttcgtcg acgcatcgac
     5461 ctgtgcagcg agcgcgaacg ccgccgatac ccgaccggcc agattcggac tgatgagctg
     5521 tcccggcgcg gcagcttcga acaccggccg ctgcgtggtc gcgtaccgat cgagcgggtc
     5581 ggtgtcggtg gcatcggcct gcgggagccg ccacaggtca tggtcgccgt tgaacgtcgg
     5641 atcgaccgtg tcactgccgt tccccgtacc gattccgacc tgcaagtaaa gggttttcgt
     5701 cgacatcttc cacatcttgt cgagccagct ctcgccgtag gccgcctctg tcttcaaggt
     5761 ggacggcgcg gcgttaccca aggcgcgctc gctcgcatag agcaccacat cgccgtacgc
     5821 ggcggtgtgc gtgaacttca agtagtcgcc ggcgtcgtac cagccgcctt cgacgtcgac
     5881 cttcggcgcg ccgttgatct tgaccagcgg tgaaccggga acgagtgagt cgtcactcgt
     5941 cgtgtcgttg aagtctgggg tttcgtacac cgtcgcgtgg gcgtctccga ggtgagctgg
     6001 ctgccggtcg agcggcccgg cgacgacgtc ggcgccgtcg cgctgcacct ggtagaagtt
     6061 gacgccgtct tcgaccacct ggccatagag cgcggcgggc gtctcgatcg cgaagtcggg
     6121 tgactgcgcg ctggcgtcgc cgtgcgcgac caggtgatag gtaccgagcg gggcgactcc
     6181 ggagaacgtg atgtcgtaca cgtcggggta gttcgcgttc cacggtccgc ggttggtggt
     6241 gccgagtttg gcttcggcga cgacctggcc gccgctcacg acgtcgaccg ttgcgtgctt
     6301 caccttgtgg cttgccatca gataggcgtg cttggcatcc gtaggcaggt aaccgacctg
     6361 gtcgacccgg atcacggcag gtggctgctt cgtggacgcc ggcttcgcgc tggcgctcgc
     6421 ggtggcgaac gcctgcgggg cggctccgaa cgtgccgagg agcgcggccg ccacggccag
     6481 cgagctcgcg atgcggacgg ggcgggtacg gaccggactt gagcggggca tgacgggccg
     6541 acctgactgt taggaagcct tcctaccgac ttcggcaggc tagatccccg gaacagcacc
     6601 g
//
LOCUS       DASWVF010000024         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622 k141_828,
            whole genome shotgun sequence.
ACCESSION   DASWVF010000024 DASWVF010000000
VERSION     DASWVF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VGD55_00415"
     CDS             complement(<1..1109)
                     /locus_tag="VGD55_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0868836.1"
                     /translation="MRTLVARDRSTEGTTVRRRLAAGTALAVASAAAVVLGTSVPAFA
                     SGVTAVTGAATPPSAGATAQYTITFTATSPLTAGVGTITINEPAGTNTPALASDYEVN
                     GSLLLINPVVSGGVGGSITLTTPVNITAGEAVVIGAVNITNPPAGPQTLVVTTSSDTT
                     PAQNSTPYIITAAGGPQVTSVTASAAPTTAAAMATYTVGFHATTALTAGIGTITLNGQ
                     GATNFPGTATSYTVNGTAVSAAPSSSGASVTLTTPVGVLANGSVTVVANGVTNPAAGI
                     HTLTVSTSGDATIATSGFFFMSGGSGGGGGGGGSGGTSPLTPVRLFGADRFGTAIAAS
                     QAEFPTAHSAGAVVLARSDDYPDALVGTALAAAKHA"
     gene            complement(1354..>2130)
                     /locus_tag="VGD55_00420"
     CDS             complement(1354..>2130)
                     /locus_tag="VGD55_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0868837.1"
                     /translation="SDDYPDALVGTALAAARNAPLLFANGGALTAGTVAEIQRVLPTG
                     GTVYLLGGLAAVPASVATTLTGLGFVVVRYAGTDRFGTSLAVADALGDPSTVLLATGI
                     NFPDALAAGPAAAHVAGVVLLTNGSSLTTAVQAYLTGHPGKVYAIGGPAVAADPAATP
                     LSGADRYGTAAAVAAALFTGPNNVGVASGTQFPDALTGGAFQAHFGGPIVLTDPGTLP
                     VPTSTYLTGAEATIVTTSIFGGTAALSTAVQASISTALGL"
BASE COUNT          353 a          648 c          774 g          355 t
ORIGIN      
        1 gcgtgcttgg ccgctgcgag tgcagtgcca acgagagcgt cggggtagtc atcggagcga
       61 gcgagcacga ctgcaccagc gctgtgtgca gtcgggaact ccgcctgcga ggcagcaatg
      121 gcggtcccga atcggtcggc accgaacaga cgcacagggg tcaacggcga agtgccgccg
      181 ctgccaccgc cgccaccacc gccgccacta ccaccggaca tgaagaagaa gccgctggtg
      241 gcaatcgtcg cgtcgccaga ggtggagacg gtcagagtgt ggatgcctgc ggccgggttg
      301 gtgactccgt ttgcgaccac ggtgacagag ccattcgcaa gcacgccgac gggggtcgtg
      361 agtgtgacgc ttgcgccgct actcgatggc gcagcagaaa cagccgttcc gttgacggtg
      421 tagctcgtcg ctgtccctgg gaagttcgtg gcaccctgac cgttcagggt gatcgtgccg
      481 atcccggcgg tgagtgcggt cgtcgcgtgg aagccgaccg tgtacgtcgc catcgctgcg
      541 gctgtcgtcg gcgccgcgga agcggtcacg ctcgtgacct gtggtccgcc ggccgccgtg
      601 atgatgtacg gcgtcgagtt ttgtgctggc gtcgtgtcag aagaggtggt gaccacaagc
      661 gtctgcgggc ctgcgggtgg gttcgtgata ttgacggcgc caatcacgac ggcttcgcct
      721 gcggtgatgt tgaccggcgt ggtgagggtg atgctgccgc cgacgccacc gctgacgacg
      781 gggttgatga ggagcaacga cccgttgacc tcgtagtcac tcgccaacgc cggcgtgttc
      841 gtgcccgcgg gttcgttgat ggtgatcgtg ccgacgccgg ccgtcagggg gctggtggcg
      901 gtgaaggtga tcgtgtactg ggcggtggca cccgcggacg gcggcgttgc agcgccggtc
      961 accgcggtca ccccggaggc gaacgcaggg acgcttgtac cgaggacgac ggcggcggcg
     1021 cttgcgacgg caagcgcggt acctgcggcg agacgccgac gaaccgtcgt cccttcagtt
     1081 gaccgatccc gtgctacgag cgtgcgcaca acaagcctcc agtgagatgc aactaatacg
     1141 ggtttcgcgg atgggcagtc gcctttatcg gcggcgatcg gccctgagca cacgctacgg
     1201 gtgcgagagg tccggacagg ccgaggcgcg ttgactagcg gacagtctcc cacggatcgg
     1261 gcgctcgtgt gcggataccc ggaggttgcc gcgccgcgtt acgggtgttg cagtccagcc
     1321 gtctcgccgc gatggcgacg agacggccgg acgtcagagg ccgagcgctg tcgagatcga
     1381 cgcctgaact gctgtggaga gtgccgcggt cccaccgaag atgcttgtcg tgacaatcgt
     1441 cgcttcagcg ccggtcagat acgtgctcgt gggcaccggc aaggtgccgg ggtcggtgag
     1501 cacgatcgga ccaccgaagt gtgcttggaa ggccccgcca gtgagtgcgt cagggaactg
     1561 cgttccactg gcgaccccta cgttgttggg tccggtgaac aaggctgctg ccaccgctgc
     1621 tgctgtgcca tagcggtcag caccggacag cggggttgct gccggatcgg cggcaactgc
     1681 gggcccgcca atcgcgtaga ccttgccggg gtgcccggtc aggtaggcct gcacggctgt
     1741 cgtgagcgag cttccgttgg tgagcaaaac gactccggca acatgtgcag ctgccggacc
     1801 ggctgccagc gcgtcgggga agttgatccc ggtcgcaagc aggacggtcg aggggtcacc
     1861 aagcgcgtcg gctaccgcga gtgaagtgcc gaatcgatcg gtgccggcat agcgcacaac
     1921 cacaaagccg agaccagtca acgtggtcgc cacactcgcc gggacggccg cgaggcctcc
     1981 aagcaggtac accgttccac cggttgggag tacccgctgg atctcggcca ctgttccggc
     2041 tgtcagtgca ccgccgttgg cgaacaagag gggtgcattc ctggccgccg caagtgccgt
     2101 accgacgagg gcgtcggggt agtcatccga
//
LOCUS       DASWVF010000025         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_255628, whole genome shotgun sequence.
ACCESSION   DASWVF010000025 DASWVF010000000
VERSION     DASWVF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255628"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGD55_00425"
     CDS             complement(<1..461)
                     /locus_tag="VGD55_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0868838.1"
                     /translation="MSTSPDVHANDGLEPATPDVDVPAADVPAADVPDVDVLGLDIAS
                     LDIPIVSARSEQAASDRARARVLFAEMVSLPEGSPERHAIRDQLVETHLPLVEYLARR
                     FRNRGELHDDLVQVATIGLIKSVDRFDLERGVEFSTYATPTIVGEIKRHFR"
     gene            complement(458..829)
                     /locus_tag="VGD55_00430"
     CDS             complement(458..829)
                     /locus_tag="VGD55_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY0868839.1"
                     /translation="MSVRVPAAAAYLSVLRTTTASLAARLDFTLDEIEDLRIAVDEAS
                     CLLLAGAVEGAELTSVFAMSTDSIQISVSTMTDKGHTPSRDSFAWTVLTALAGEVDAW
                     TEADGQQTISLIKHRSRDLLT"
     gene            complement(991..1494)
                     /locus_tag="VGD55_00435"
     CDS             complement(991..1494)
                     /locus_tag="VGD55_00435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0868840.1"
                     /translation="MIRPARPDDVPTILALIRELAAYEREPDAVVATEAGVEAALFGD
                     RPALFGLIAEHPSADGPVIAGFALWFLNFSTWVGRHGIYLEDLYVRPELRGRGYGGEL
                     LVELARIADERGYGRLEWAVLDWNESAHRFYASLGAVPMQEWTIWRVSGEALPALAKR
                     PDADGRA"
BASE COUNT          287 a          538 c          553 g          276 t
ORIGIN      
        1 tcgcggaaat gcctcttgat ttcacccacg atcgtcggcg tcgcgtaagt cgaaaactcg
       61 acgccacgct ccaggtcgaa tcggtcgacg gacttgatca atccgatcgt ggcgacctgt
      121 accaagtcgt cgtgcaactc accacgattg cggaaccggc gggcgagata ttcgacgagt
      181 ggcaagtgcg tctcgacgag ctggtcgcga atggcgtgcc gctcggggga gccttcgggc
      241 aggctcacca tctccgcaaa aagtacgcgg gcgcgggccc gatcgctggc agcctgctct
      301 gagcgggcgc tgacgatcgg gatgtcgaga ctcgcgatgt cgagaccgag aacatcgaca
      361 tcgggcacat cggcagcagg cacatcggca gccggcacat cgacgtcggg cgtcgcaggt
      421 tcgagcccat cgtttgcgtg cacgtcgggg ctagtgctca cgtcagcagg tccctgctcc
      481 ggtgcttaat caacgagatc gtctgctgcc cgtcggcttc ggtccacgcg tcgacttcgc
      541 cggcgagggc agtcaagacg gtccaggcga acgagtcgcg gctcggcgtg tgacccttgt
      601 cggtcatcgt cgaaaccgag atctggattg agtcggtaga catcgcgaag acactcgtga
      661 gctcggcgcc ctcgaccgcg cccgccagca gcaggcacga cgcctcgtcg accgcaatgc
      721 gcaggtcttc gatctcatcc agggtgaaat cgagccgcgc cgccaggctc gccgtcgtgg
      781 tgcgcaggac cgagaggtag gcggcggccg ccggcactcg cacggacacg aaatcaccgc
      841 cggtgcggcc tcgggccggc cgcgccttgg tgtctgagtc cgctgccgga ccgccctctg
      901 tggtcgacac gcgtcgcgct ccatgtgtgt gcaggtattg ccaggtttgc cgggcttcac
      961 ggtaccgcgc ggttgccgaa ctgtacgtga ctaggcgcgc ccgtcggcgt ccggccgttt
     1021 cgcgagtgcc ggcaacgcct cgccgctcac gcgccagatg gtccattcct gcatggggac
     1081 ggcgccgagc gacgcgtaaa aacgatgcgc ggactcgttc cagtcgagaa ctgcccactc
     1141 gagccgaccg tagccgcgct cgtcggcgat ccgcgcgagc tcgaccagaa gttcgccgcc
     1201 gtaccccctg ccgcgcaact ccggtcgcac gtagagatcc tcgaggtaga tgccgtggcg
     1261 acccacccac gttgaaaaat tcaggaacca cagggcgaaa ccggcgatga ccggtccgtc
     1321 agcagagggg tgttcggcga tcaggccgaa cagcgccggc cgatctccga acaatgcggc
     1381 ctcgacaccg gcctcggtcg cgacgacggc atcgggctcg cgttcgtacg ccgcgagctc
     1441 acgaatcagg gccaagatcg tcgggacgtc gtcgggtcgg gctggtcgga tcacgctgac
     1501 cggatccaca cggacagcag gcttggctcg agggtcgccg agatggttcg cgacaggtcg
     1561 tcgcagacgg tcgcggggtc gacctgatcc ttctgcctgg aagcgaaggc ggtgatcgtg
     1621 gagatggcgt tgtatctcgc tcggttgaac cgcc
//
LOCUS       DASWVF010000026         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_331937, whole genome shotgun sequence.
ACCESSION   DASWVF010000026 DASWVF010000000
VERSION     DASWVF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331937"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGD55_00440"
     CDS             <1..517
                     /locus_tag="VGD55_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0868841.1"
                     /translation="AALAPGSARDSETGVLFYLVAYGFMTIGSFAIVMLVRDGDGEAN
                     HLSRWVGLGRRSPLVAGIFAFFLLALAGLPGTSGLWAKVAVFKAAGEGGAWPLVIVGV
                     GASAVTAYYYVRIIVLMFFQEPLPDGPTVVLPQFFTSTAIGLGLAVTLVLGVIPQPLL
                     NLAGKAGLFIR"
     gene            549..1547
                     /locus_tag="VGD55_00445"
     CDS             549..1547
                     /locus_tag="VGD55_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0868842.1"
                     /translation="MSSEGPLGLMIPDAALESEIRTGIDAVESMLRDSVKSDYPFVTE
                     TSRHLVEAGGKRFRPLLVLLAAQFGDPAAPGVLPAAVVVELTHLATLYHDDVMDEALL
                     RRGTVSANARWSNTIAILTGDFLFSRASDLLADLGPEAVRIQARTFERLVQGQIRETT
                     GPDEGVDPLEYYLQVVADKTGSLISTSARFGAMFAGAPEPVAEIMIRYGERIGVAFQL
                     SDDVLDVTSDPTGFGKTPGTDLREGVVTLPVLHARRSRDPADARLLELLDTDLTDDDR
                     HRETLRLLRLHPAIEAARADLDRWANDARLTLAPLPDVPARRALEALCDFVVARTR"
     gene            1708..2808
                     /locus_tag="VGD55_00450"
     CDS             1708..2808
                     /locus_tag="VGD55_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0868843.1"
                     /translation="MATASTGIGGTNVAHGAMGTGGATGHGAALLGITIRDVAREAGV
                     STATVSRALRGLPNVDPVTREHVRRVADRLDYVISPAASRLAGGRAGSIAVITPFLGR
                     WYFSKVLSGVERVLQRSDLDLLLYSTGDPSEEHRVPPHKRLRRRVDGVLVIGLPADSP
                     DLQELFELEMPVTLIGSHSVGVTSVSIDDVEGGRLATQHLVNLGHERIGLISGRPLPT
                     PFFPENDRLAGYRKALEASGLTVEPGLQVPGYFTIEGGEHAMTAQLAQRTPPTAVFAM
                     SDEMAFGALRALRRHGMRPGHDVAIVGFDGHEMSDLLDLTTVCQPAEELGSVAARCLL
                     DLLDDPAREAEVQRLPTQLCVRGSTTLHRIVT"
     gene            3087..>3860
                     /locus_tag="VGD55_00455"
     CDS             3087..>3860
                     /locus_tag="VGD55_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0868844.1"
                     /translation="MSFSLPTGRRRRRLMVVIAPTVAAVTLLAACSSSKKAAAPASSS
                     APVATTSASTAPSASTPVAAASGSAAPQATTCADYAQYTSDATKGKTVTMFGSILPPE
                     SDSLNTSWAQFESCTGIKIAYEGSNDFESQLPVRVQGGTAPDLAIIPQPGLLAQMVAL
                     NAVKAPPAGVVANEDKYFNAAWKSYGSVNGTFYAAPQSANMKSLVWYSPSAMSKAGYT
                     IPTTWDQLAALSDKIAASGTKPWCGGIGSGTATGWPATDW"
BASE COUNT          600 a         1289 c         1249 g          722 t
ORIGIN      
        1 tgctgccctc gcgccgggca gtgcgcgcga cagcgaaact ggcgtgttgt tctacctggt
       61 cgcatacgga ttcatgacga tcggctcgtt cgccatcgtg atgctcgtac gtgacggcga
      121 cggcgaggcg aatcatctgt cgcgatgggt cgggcttggt cgccggtcgc cgctggtcgc
      181 gggcatcttt gcgttcttct tgctggcgct agctggattg cctggcacga gcgggctatg
      241 ggccaaggtt gccgtcttca aggccgcggg cgagggcggc gcctggccgc tggtgatcgt
      301 tggcgtcggt gcaagcgccg tgaccgcgta ctactacgtg cggatcatcg tcttgatgtt
      361 cttccaagag ccgctccccg acggccccac cgtcgtcctc ccccagttct tcacctcgac
      421 cgccatcggt ctcggccttg cggttacctt ggtgcttgga gttattccgc agcccctgct
      481 caaccttgca ggcaaagcgg ggctcttcat ccgctagaac gtatccatca gggcaggagg
      541 tcttcggcgt gagcagcgaa ggcccactcg gactcatgat ccctgatgcg gctctcgagt
      601 cggagatccg cacgggcatc gatgcggttg agtcgatgct gcgcgactcc gtcaagagcg
      661 actacccgtt cgtcaccgag acgtcgaggc acctggtcga agctggtggg aagcggttta
      721 gaccgttgct cgtccttctc gcagcccagt tcggcgatcc ggccgcacct ggtgtgctgc
      781 ccgccgccgt ggtcgtcgag ttgactcacc tcgcgaccct gtaccacgac gacgtcatgg
      841 acgaagctct gctgcgacgc ggcacggtga gtgcgaatgc gcggtggtcg aacaccatcg
      901 cgatcctgac tggcgacttt ctgttctcgc gtgcgtctga tctgctggcc gatctcggcc
      961 ccgaggcggt gcgcattcaa gcgcgcacgt tcgagcggct cgtccagggt cagatcaggg
     1021 agacgaccgg ccccgacgag ggcgtcgatc cgctcgagta ttacctccag gtcgtggccg
     1081 acaagaccgg ttcgctgatc tcgacgtcgg cacgtttcgg agcgatgttt gctggcgcgc
     1141 cagagccggt tgccgagatc atgattcgct acggcgagcg gattggtgtc gcgttccagc
     1201 tctccgatga cgtgctcgac gtcaccagcg acccgaccgg gttcggcaaa acgccaggta
     1261 ccgatcttcg cgagggtgtc gtgacgctgc cggtcctgca cgcccgtcgc tcccgtgacc
     1321 cggccgatgc tcgcctgctc gagcttctcg acacagacct gaccgacgac gaccggcacc
     1381 gcgaaacgct tcgcctcctt cgcctgcacc ccgcgatcga ggcggcccga gccgatctcg
     1441 accggtgggc gaacgacgcc aggctcacat tggcgccgct tcctgacgtt ccggcccggc
     1501 gcgctctcga ggcgctgtgc gatttcgtgg tggcccgcac tcggtgactg accggacctg
     1561 atcgccccgg cagacggagc cggagctttc cggtcagttt tcgtccctga ggatgacacc
     1621 gcttacaaaa cggctatcgt tccggttcac aaccccgtcg gggctcgctg tcgtccgctg
     1681 aacacgctct caggagagga tctccgggtg gccactgcca gcactggcat cggtggcact
     1741 aacgtcgccc atggcgccat gggaaccggt ggcgccaccg gtcacggagc cgcgctgctc
     1801 ggcatcacga tccgcgacgt cgcgcgtgag gccggagtct cgaccgcgac cgtttcccga
     1861 gcgttgcgtg gcctgccgaa cgtcgacccc gtcactcgcg aacacgtgcg ccgcgttgcc
     1921 gaccgtctcg actacgtcat atccccggcc gcgtcgaggt tggcgggtgg gcgggccggc
     1981 agcatcgccg tcatcactcc gttcctgggc cggtggtact tctcgaaggt tctgagtggt
     2041 gtcgagcgcg tcttgcagcg ctccgaccta gatctgctcc tctacagcac gggcgatccc
     2101 agcgaggagc accgagtccc gccgcacaag cgcctgcggc ggcgagtcga cggcgttctg
     2161 gtgatcgggc tgcccgctga cagtcccgac cttcaagagc tgttcgagtt ggagatgccg
     2221 gtgacgctca tcggctccca cagcgtgggt gtcacgagcg tctcgattga cgacgtcgag
     2281 ggcggtcggc tcgcgaccca gcacctggtt aacctcggcc atgagcggat cgggctcatc
     2341 tcgggtcgcc cgctgccgac gccgttcttc cccgagaacg accggctcgc cgggtaccgg
     2401 aaggcactcg aggcctccgg cttgactgtc gagccggggt tgcaggtgcc gggttacttc
     2461 acgatcgagg gcggcgagca cgcgatgacc gcccaactcg cgcagcgcac gccgcctact
     2521 gcggtcttcg cgatgtcgga tgagatggcg ttcggagcgc tacgcgcgtt acgccggcac
     2581 gggatgcgcc ctggtcacga cgtcgcaatt gtcggtttcg atggtcacga gatgtcggat
     2641 ctgctcgatc tgacgaccgt gtgccagccc gcggaagaac tcggctcggt cgcggcgcgg
     2701 tgcctgctcg acctgcttga cgacccagcg cgggaggccg aggttcaacg gctgcccacc
     2761 cagttgtgcg tgcggggctc gacgacgttg caccggatcg tcacctgagg gtctgcgcta
     2821 cctatttgcc ccagaccggg aacgttgtag tttcgagacg gttcctggtg gatgttcggt
     2881 gggccgcggg gtaggtcggc agtggtacgg gtggcggcaa aggaggggca tccgagccca
     2941 atttgtggga acgatgccaa accgtaaccg tatacatcgc aattgttgcc cgtcacgcct
     3001 tgatctcgct ggctttgagg gccacgatgt caccgtttgc agccgtgcgg gcttcccgtc
     3061 cggccccagt cgcaagagga gaaaccatga gtttcagctt gccaaccggc aggcgccgtc
     3121 gccgcctgat ggtcgtcatt gcgccgaccg tcgccgcggt cacgctgctc gctgcctgtt
     3181 caagcagtaa gaaggcagcc gcgccagcca gtagctccgc ccctgtcgcc accacgtcag
     3241 cgtccaccgc cccttccgcg tcaacgcctg tagctgccgc tagcggcagc gccgcgccgc
     3301 aggccaccac ctgcgccgac tacgcgcagt acacgagcga cgcgaccaag ggcaagaccg
     3361 tcacgatgtt tggctcgatc ctgccgcctg aatccgactc gttgaacacg tcgtgggcgc
     3421 agttcgagag ctgcaccggc atcaagatcg cctatgaggg atccaacgac ttcgagtccc
     3481 agctgccggt tcgcgttcaa ggtggcaccg cgcctgacct cgcgatcatc ccgcagcctg
     3541 gcctgctcgc tcagatggtt gccctcaatg ccgtcaaggc cccgccggct ggcgtcgtgg
     3601 ccaacgaaga caagtacttc aatgccgcat ggaagtcgta cggctcggtg aacgggacct
     3661 tctacgccgc gccgcagagc gcgaacatga agtcgctcgt gtggtactcg ccgtccgcga
     3721 tgtccaaggc gggctacacg atcccgacca cgtgggacca gctcgcagcc ctgagcgaca
     3781 agatcgccgc atccggcacc aagccgtggt gtggtggcat cgggtcagga accgcgaccg
     3841 gttggccagc cacagactgg
//
LOCUS       DASWVF010000027         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_484848, whole genome shotgun sequence.
ACCESSION   DASWVF010000027 DASWVF010000000
VERSION     DASWVF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484848"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VGD55_00460"
     CDS             <1..1149
                     /locus_tag="VGD55_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEY0868845.1"
                     /translation="DHLVHNASRGIAVKPCQELLRASIGLPWSIVIKYLGSKRLLASI
                     LGDIAAAVGAHTALDLFTGTTRVAQEFKRRGVDVTAVDCATYADVLARCYIATDATVV
                     DKDELARAIDELNALPGRPGYFTETFCVASRYFQPKNGARVDVMRDAIEARYRSDPLY
                     PVLLTALMLAADRVDSTTGLQMAYLKQWAARAHNDIELRAPELLAGPGTAMRGDAMSV
                     VDDVRHVDLAYLDPPYNQHRYFTNYHVWETLVRWDAPPHYGVACKRIDSRNDDTHSPF
                     NGKRSMPGAMHSVISRAKADVVVVSYNDESWVTTEQMSRWLKDAGHADVAILAFDAKR
                     YVGAQIGIHNPAGEKVGSVSHLRNTEYIFVAGSTEHVDAATWAVGGCR"
     gene            complement(1146..>2540)
                     /gene="dctP"
                     /locus_tag="VGD55_00465"
     CDS             complement(1146..>2540)
                     /gene="dctP"
                     /locus_tag="VGD55_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY0868846.1"
                     /translation="AVTTITLELPDTGDPDGTYFAADVEKRSQGTLKVVVDSSTYSTA
                     DSTYGAHLVAAMRARRVNFSYDPARDWAAAGVAGFEALDAPFLLTTNAASASLANSSL
                     VPAILGQLSSLGLIGLGLIPTEPREILSVAPLFPLSAFHGVSLRIVDNPETSALISAI
                     GADPVQGLSADETGSELQDRQILAAETQPWAIEGNSYNVEAPYLTTYGLFPKFDIIVT
                     TKSAWATLTRAEQTAFREAAVDTLVNSRQVPARESTELTTLCAAGMVLDQPSTAQLSA
                     ISGAAMRATPAGAEVASMIGKIRSTIADTGPQLDAVTPPASCHVAHTVAEATAMANAS
                     TLQSPAPPASATSTSGATIPPGVYVTTDTVADFRAAGQVGDDWNTAKTITLNLFADGI
                     ASEGEEPGGGGEFGTYVVHGEQVTFTWSADTGLTPQVFRWSYLNGLLTFTVVNVEDGG
                     SRVIYSAHPWRKVG"
BASE COUNT          464 a          791 c          809 g          476 t
ORIGIN      
        1 gaccatttag tccataacgc gtcgcgtggg atcgctgtta agccctgtca agagctgttg
       61 cgagcctcga tcggcctacc ctggagcatc gtgatcaagt acctcggctc caagcggctg
      121 ctggcctcga ttctcggcga catcgccgct gcggtaggcg cccacacagc acttgatctc
      181 ttcaccggga cgaccagggt tgcgcaggag ttcaagcggc gaggggtcga tgtcacggcc
      241 gtcgactgtg caacgtatgc cgacgtactc gctcgttgtt acatcgcaac cgatgcaact
      301 gtcgtcgaca aggacgaact cgcacgcgcg atcgacgagc tgaacgcgct tccgggcaga
      361 cctggttatt tcaccgagac gttctgcgtg gcgtcgcgtt acttccagcc gaagaacggt
      421 gcccgcgtcg acgtcatgcg cgatgcgatc gaagcccggt atcgcagcga cccgctatac
      481 ccggtgttgc tgacggcgct catgctcgct gccgaccgcg tcgactcgac gaccggtttg
      541 cagatggcct acctcaagca atgggcagcg cgcgcgcaca acgacatcga actgcgcgcg
      601 cccgagttgc ttgccggccc tggcacggcg atgcgcggtg acgcgatgtc tgtcgtcgac
      661 gacgttcggc acgtcgatct cgcctacctt gatcctccgt acaaccagca ccgctacttc
      721 accaactatc acgtgtggga aacgctcgtt cgctgggacg ccccgccgca ttacggcgtc
      781 gcatgcaaga gaatcgattc gcggaacgac gacacgcatt cgccgttcaa cggcaagcgt
      841 tcgatgcccg gtgcgatgca ctcggtgatc tcgcgtgcca aggccgacgt ggttgtcgtc
      901 tcctacaacg acgagtcgtg ggtgacgaca gagcagatga gtcgctggct caaagatgcg
      961 ggccatgcgg acgtcgcgat cctcgcgttc gacgccaagc gttacgtcgg tgcgcagatc
     1021 ggaattcaca accccgctgg cgaaaaagtc ggctcggtgt cgcacttgcg caacaccgaa
     1081 tacatcttcg tggccgggtc gactgaacac gtcgacgcag cgacctgggc agtaggcggc
     1141 tgtcgctagc ccaccttgcg ccacggatgt gccgagtaga tgacccgact gccgccgtcc
     1201 tcgacgttga cgacggtgaa agtaagcagg ccgttgaggt agctccagcg aaagacctgg
     1261 ggcgtgaggc ccgtgtccgc ggaccaggtg aacgtcacct gttcgccgtg cacgacgtag
     1321 gtgccgaatt cgccgccgcc gccgggttcc tcgccctcgg aagcaatgcc gtcggcgaag
     1381 agattcagag tgatggtctt cgcggtattc cagtcatcgc cgacctgacc ggctgctcta
     1441 aagtcggcaa cggtgtctgt cgtgacatac acgcctggcg ggatcgtcgc accgctagtg
     1501 ctggtcgcac tcgcgggcgg ggcagggctc tggagcgtcg acgcgtttgc catggccgtt
     1561 gcctcggcga ccgtgtgtgc cacgtgacat gaggcaggcg gcgtcacggc gtccaactgc
     1621 gggccggtgt ccgcaatcgt cgaccggatt ttcccgatca tcgacgcaac ctcggcgccc
     1681 gcaggggtcg ctcgcattgc tgctccggag atcgcgctta attgcgcagt cgacggctgg
     1741 tcgagcacca tgcctgcagc gcacagcgtg gtgagctcag tactctctcg tgccggcacc
     1801 tgacgagaat tgacgagggt gtcgacggca gcttcccgaa atgctgtctg ctcggctcgc
     1861 gtgagggtcg cccacgcgga cttggttgtg acgatgatgt cgaatttagg gaacagtccg
     1921 taggtcgtca gatagggagc ctcgacgttg taggagtttc cctcgatggc ccatggctgg
     1981 gtctcagcag caaggatttg acggtcctgc agctccgacc cggtttcgtc cgcggatagg
     2041 ccctgcaccg gatcggcacc gatcgctgaa atcagagcag acgtttcggg gttatcgaca
     2101 atgcgaagcg agaccccgtg aaacgccgag agcgggaaca gcggtgccac cgacaggatc
     2161 tcgcgtggct cggtgggaat caggccgagt ccgatcaaac cgagactaga cagctgacca
     2221 aggatggccg gtacgagcga actgttggcc agcgaggcgc tcgcggcatt tgtcgtcagg
     2281 aggaacggtg cgtccaacgc ctcgaagccg gccacgcccg ccgccgccca atctcgcgcc
     2341 ggatcgtaac tgaagttcac ccgccgcgct cgcatcgcag cgacgagatg tgccccgtac
     2401 gttgagtcag cggtgctgta ggtgctcgag tcgacgacga ccttgagcgt tccctgcgag
     2461 cgcttttcga cgtccgctgc gaagtaggtg ccatcaggat cgcccgtatc tggtagctca
     2521 agcgtgatcg tcgtgacggc
//
LOCUS       DASWVF010000028         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_331955, whole genome shotgun sequence.
ACCESSION   DASWVF010000028 DASWVF010000000
VERSION     DASWVF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331955"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VGD55_00470"
     CDS             <1..623
                     /locus_tag="VGD55_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0868847.1"
                     /translation="SVMGSPEIAAAKMSLRRHVLARRGRHSDVERAQIAAALATAFAA
                     EFGSRLLGAGQPGEIVDVHRPPATANAHDAAITTVALYLSFATEPGTGPVIDWLTGRR
                     VRALAPTLLPDADLGWMDIPDGPPLGLDAIIDAQVILVPALAIDPSGHRLGRGGGSYD
                     RALTRVRPGQTVLAVVHDDEVFDVVPFEPHDRIVDGALTPSGVRYF"
     gene            complement(638..1414)
                     /locus_tag="VGD55_00475"
     CDS             complement(638..1414)
                     /locus_tag="VGD55_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0868848.1"
                     /translation="MRLSRPLIAGFGVVGLLLVAAAIGQFAPTSPKKPSLSIGTTLNQ
                     ALPAWVSSVEFTDDTGKQLTLASFKGKAVVFTPFMTLCQETCPLTTSNMQLLDQSVIK
                     AHQEHNVQMVMITIDPARDTIARLHAYRSAAQITQPNWTLLTAAPADLTALWKSLGVD
                     VEKTAEDNPPGIDWLTGKPLTYDLSHTDEVFMLNKSGNERFLIDGMPNTQGVAPPPIL
                     DKFLSDEGRDDLAHPDGSTWTVADATTSLAWVLGKHLPKA"
     gene            complement(1404..>2280)
                     /locus_tag="VGD55_00480"
     CDS             complement(1404..>2280)
                     /locus_tag="VGD55_00480"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0868849.1"
                     /translation="IRWTVSFLAGNAVIVIATQSSIGAYDDVLFWIHMVQHVLLLMVA
                     PPLLLLGRPVTLLLHATRNPVHTTVKRIVRSAPINVITFPLLGVLLYIATVVGTHLTG
                     FMNLTLQHPVAHDLEHVLYLVVGYLYFLPLIGREPIRWRLSFTTRLFLLVLAMPVDTF
                     TGVVLTQTNHELFPGYAGRRNWGPSLVADLHAGGAVMWIGGDFVMLVMVLILFGGAAR
                     SRRPLDGGAWVESARQNSLAGGALVGTVSQALDDDDAQLAAYNSYLQTLGGDAHSQSP
                     GRESHLQSLGGDDAT"
BASE COUNT          423 a          729 c          753 g          375 t
ORIGIN      
        1 gatcggtgat gggctcgccg gaaatcgccg ccgccaaaat gtccctgcga cgccacgtgc
       61 tcgcccggcg cggccgccat tccgacgtcg aacgcgcgca gatcgcggcc gcgctcgcca
      121 ctgcgttcgc ggccgaattc gggtcccgat tgctgggcgc cggccagccg ggggaaatcg
      181 tggacgtgca ccgcccccca gcgacagcaa acgcccacga tgctgccatc acgaccgtgg
      241 cgctgtacct ctcgttcgcg accgagccag gtaccggtcc ggtgatcgac tggctaaccg
      301 gtcggcgagt tcgggctctc gcaccgacgc tgctgcccga cgcggatctc ggctggatgg
      361 acatacccga cggacctccg ttgggactcg acgcgatcat cgacgcgcag gtgatcctcg
      421 ttcccgcact cgcgatcgat ccgagcggac accggctcgg ccgcggcggc ggctcgtacg
      481 acagagcatt gacgcgggtg cggcccgggc agaccgtgct cgccgtcgtc cacgatgacg
      541 aagtgttcga tgtggtcccg ttcgaaccac acgatcgcat cgtcgacggt gcgctcactc
      601 cgagcggggt gcggtacttc tgacccgacg cgcgggccta cgccttgggg agatgcttcc
      661 cgagcaccca agcgagcgag gttgttgcgt cggccacggt ccacgtggac ccgtcgggat
      721 gcgcgagatc gtcacgaccc tcgtcgctga ggaacttgtc gagaatcggc ggcggcgcaa
      781 caccttgcgt gttgggcatc ccgtcgatca agaagcgttc gttgcccgac ttgttgagca
      841 tgaacacttc gtcggtgtgg ctgaggtcgt aagtgagcgg cttgcccgtc agccagtcga
      901 tgcccggggg gttgtcctcg gcagtcttct cgacatcgac gccgagtgac ttccagagcg
      961 cagtgagatc agcaggcgcg gccgtaagca gcgtccagtt cggctgggtg atctgcgcgg
     1021 ccgaccggta ggcgtgcaac cgcgcgatcg tgtcccgcgc cggatcgatc gtgatcatca
     1081 ccatctgcac gttgtgctct tggtgcgcct tgatgaccga ctggtccagc agctgcatgt
     1141 tgcttgtcgt gagcggacac gtttcctggc acagcgtcat gaaaggcgtg aacaccacgg
     1201 cctttccctt gaacgaagcc agcgtcaact gctttccggt gtcatcggtg aactcaaccg
     1261 acgacaccca tgcgggcagc gcttggttga gcgtcgtccc gatcgagagg ctcggctttt
     1321 tcggggacgt cggggcgaac tgtccgatcg ccgccgcgac gagtaggaga ccgacgacac
     1381 cgaaacctgc gatcaacggg cggctaagtc gcatcgtcgc cgccgagcga ttgcaggtgg
     1441 gactcgcgac cgggcgactg tgagtgggcg tccccaccga gggtttgcag gtaggagttg
     1501 tacgccgcga gctgagcatc gtcgtcgtcc aaagcctgcg aaaccgtgcc gacgagagcc
     1561 ccaccagcca gcgagttctg gcgagccgat tccacccacg caccgccgtc gagtggacga
     1621 cgcgaacgag cggcgccgcc gaacaaaatc agcaccatga ccagcatcac gaagtcgccg
     1681 ccgatccaca tcacggctcc gccggcatgc aggtcagcga ccaagctagg cccccagttt
     1741 cgacgtccgg catagccagg aaacagttcg tggttcgtct gggtcagcac cacgccggtg
     1801 aacgtgtcga ctggcatcgc gagcacgagc aggaacaacc gagtggtgaa cgacaggcgc
     1861 caccggatcg gttcccgtcc gatgagcggc aggaagtaga ggtagccgac gacgaggtag
     1921 agcacgtgct caaggtcgtg ggcgaccggg tgctgcagcg tcaggttcat gaagccggtc
     1981 aggtgcgtgc cgacgacagt cgcgatatag agcaggacgc cgagcagcgg aaacgtgatc
     2041 acattgatcg gtgcggaacg cacgatgcgc ttgacggtcg tgtgcaccgg attgcgagtc
     2101 gcgtgcaaca gcaacgtgac cgggcgcccg agcaagagca gcggcggcgc gaccatcagc
     2161 agcaacacgt gctgcaccat gtggatccag aacaacacgt cgtcgtaggc gccgatactg
     2221 ctctgcgtcg cgatcacgat cacggcgttg ccagcgagga acgagacagt ccagcgaatc
//
LOCUS       DASWVF010000029         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_51797, whole genome shotgun sequence.
ACCESSION   DASWVF010000029 DASWVF010000000
VERSION     DASWVF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51797"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="VGD55_00485"
     CDS             <1..1923
                     /locus_tag="VGD55_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0868850.1"
                     /translation="AGKAPGLVATHDRAEALLIAGMPSEARESLVRRVDDVERAGFAV
                     DLAEWHLLLAQAALTEGEADVARASAQRALTEFRAQGRARWALLAQQLVVRARWASGE
                     RTAALSRAARVAHAKLWTAGWHVAALHCLIVAGRIELEAGRLSSARIDLAQAAQARRS
                     GPADLRAAAWYAEAMLRLASSDTRGATTALKAGLRVVDDHAASLGATDLRVHATGLGA
                     ELAEQGVRLAVESGKPIAVLEWIERFRAGTLRRRPVRPPSDARLAAELSDLRRVTAEL
                     TESTADGEDSRQLRAEQLRLEEAVRHRSRHARGSRLPVHKFDIDDLRAAVGQRAFVEI
                     LRVDDAMLAITLVDNRLRLHQLGSYPEAMRELESLRFSLHRLARRHGSATSLAAARLG
                     LLHAADALDALLLRPVFDVVGDRELVIVPTGQLHALPWPTLPSLRGRPVSVAPSATTW
                     LAAMRANRPTRARASRRTVLVAGPNLEHAEPEVAALARLYDSPQLFIGPRATADGVRQ
                     TMDGAELVHIAAHGRFRADNPQFSAIDLADGPLTVYDLERMHKGPRRLVLSACDSGLS
                     AVHAGDELMGLAGAMFSLGTSTLIASVVPVADDLTEALMVELHRELCTGATPADALAA
                     SQLRVQVDGFVCFGAG"
     gene            complement(1927..>2130)
                     /locus_tag="VGD55_00490"
     CDS             complement(1927..>2130)
                     /locus_tag="VGD55_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0868851.1"
                     /translation="DIETLASRFAAAGMGDDALVGAYCGSGVNAAHTAFAITLAGLPT
                     PALYVGSWSNWIADGSRPIATGA"
BASE COUNT          357 a          696 c          714 g          363 t
ORIGIN      
        1 gccggcaaag cgcctgggtt agttgctacg cacgaccgtg cagaggcgct gctcatcgcc
       61 ggcatgccga gcgaggcacg ggagtcactc gttcggcgag tcgacgatgt cgagcgcgcc
      121 gggttcgcgg tcgacctcgc cgaatggcac ctcttgcttg cacaagccgc attgacagaa
      181 ggtgaagccg atgtagcgcg tgcttcggca cagcgtgcgc tcaccgaatt tcgagcccag
      241 ggtcgagccc gctgggcgct gcttgcgcag caattggtgg tccgcgcacg atgggcgagc
      301 ggtgaacgaa cggcggcatt gtcgcgagcg gcccgagttg cacacgcgaa gttgtggacg
      361 gcggggtggc acgtggcggc gctgcattgc cttattgttg ccgggcgtat cgagctagaa
      421 gccggacgcc tttcatcggc gcggatcgac ttggcgcagg cagcacaagc acgcaggagc
      481 gggccagccg acctgcgtgc ggccgcttgg tatgcggagg ccatgctgcg cctggcatcg
      541 agcgacaccc gcggcgcgac gaccgcattg aaggcgggcc tgcgcgtcgt cgacgaccac
      601 gcggcatcgc tcggcgcgac agacctccgg gtgcacgcaa cgggtcttgg tgcggaactc
      661 gccgagcagg gcgttcggtt ggccgtagag tcgggcaagc cgatcgccgt cctcgaatgg
      721 attgagcgct ttcgcgccgg caccttgcgg cgtcgacctg tgcgaccacc ttcggatgca
      781 cgactcgccg ccgagctctc cgacctgcgc cgggtaacgg ccgaactgac cgagagcaca
      841 gccgacggcg aagattcgcg ccaactgcgc gcggagcagc tacgcctcga ggaggctgtt
      901 cgacaccgtt caaggcacgc gcgcgggagt cggctgccgg tacacaagtt cgacatcgac
      961 gatctgcggg ccgcagtcgg tcagcgagcg ttcgtcgaga tcctgcgggt cgacgacgca
     1021 atgctcgcca tcaccctggt cgacaatcga ttgcgcctac accaactggg gtcgtacccc
     1081 gaggcgatgc gcgagctcga atcgctgcgg ttctcgttgc accgattggc acgacggcac
     1141 ggatcagcga cgtcgttggc ggcagcacgg ctcggactct tgcacgccgc tgatgcactc
     1201 gacgcgttac tgctccgacc ggtgtttgac gtcgtgggcg accgcgaact ggtcatcgtc
     1261 ccgacggggc aactgcacgc cctgccatgg ccgacgttgc catcgctgcg cggacggccc
     1321 gtttctgttg cgccgtcggc gacgacgtgg ttagcggcga tgcgggcgaa taggccaact
     1381 cgcgcacgag caagtcgtcg caccgtgcta gtagcagggc caaacctcga acatgccgaa
     1441 ccagaggtcg cggctctcgc gaggctttac gactcccctc agttattcat cggcccgcgg
     1501 gccactgctg acggcgttcg gcaaaccatg gacggtgcgg agctcgtgca catcgcggca
     1561 cacgggcgat ttcgcgccga caacccgcag ttctccgcga tcgatctcgc cgacgggccg
     1621 ctgactgtct acgaccttga gcggatgcac aaaggaccgc gccgactcgt gctctctgcc
     1681 tgcgactcgg ggctctcggc cgtgcacgca ggagacgagc ttatgggctt agccggcgcc
     1741 atgttttcct taggtaccag cacattgatc gcctccgtgg ttccggtggc cgacgacctt
     1801 acggaggcgc tcatggtcga actgcaccgc gaactatgca cgggtgcgac gccggcagac
     1861 gctctcgctg catcgcagct tcgagtgcag gtcgacggat tcgtctgctt cggcgccggt
     1921 tagacctcag gcgccggtcg cgatcgggcg gcttccgtcg gcaatccagt tcgaccacga
     1981 tccgacatac agagccggag ttggcagacc tgccagcgtt atagcaaacg ccgtgtgcgc
     2041 agcgttgacg ccgctgccgc aatacgcgcc gaccagtgca tcgtcgccca tgccggcggc
     2101 cgcgaagcga ctcgctaatg tctcgatatc
//
LOCUS       DASWVF010000030         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_179192, whole genome shotgun sequence.
ACCESSION   DASWVF010000030 DASWVF010000000
VERSION     DASWVF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179192"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..365
                     /locus_tag="VGD55_00495"
     CDS             75..365
                     /locus_tag="VGD55_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868852.1"
                     /translation="MNAIRRLVVATLLAVWCFPAVSSANPSPAAPAPIAAEPASPIPA
                     TSVENNETEIGSLAARQQQARQLEDFKGGRAYILGSSVVVVLLVVLLVLLLV"
     gene            complement(406..801)
                     /locus_tag="VGD55_00500"
     CDS             complement(406..801)
                     /locus_tag="VGD55_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0868853.1"
                     /translation="MAEAGRHPHFVSPVAWHASLAEGLVAAGESKRAVLLVVGRQSCG
                     GTRALVERTIAKEEIHEYLDAHYVCVAGDADSPEPAVAELVARMPQHDRTPLCLYLSV
                     EKKLMHSTSGGRPPAVFLSDLLEAPHRSR"
     gene            853..1437
                     /locus_tag="VGD55_00505"
     CDS             853..1437
                     /locus_tag="VGD55_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0868854.1"
                     /translation="MAARRLARMARQLPLGPEGTLRLAVVADTHSQPHPRAAEYLAAR
                     APDAILHAGDIGDLAVLDQLSSIAPVLAIRGNIDVHAPDLPDVLTLDLVRSVEHPQPV
                     LRMLLVHIGVYGPKLRAEVAATARAQGAQAVVCGHSHVPFLGRDRELTVFNPGSIGPR
                     RFHLPIVFGTIDVTPAGLRFAHVDCETGAQWTPP"
     gene            complement(1695..2123)
                     /locus_tag="VGD55_00510"
     CDS             complement(1695..2123)
                     /locus_tag="VGD55_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0868855.1"
                     /translation="MTGIRVVRSPIHGYGVVALRDFAEGEAIAEVDGVAWREGENVDD
                     RYSLRIENGVYFDMVDQTRFINHSCDPNTWVDVGVTDDGEAWASVIALRPIAAGEELS
                     YDYAFPAHLAEPCRCGADNCRGLIIDEDHLVPVAGGSGSP"
     gene            2235..>2442
                     /locus_tag="VGD55_00515"
     CDS             2235..>2442
                     /locus_tag="VGD55_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868856.1"
                     /translation="MQATAAATRFSAGSVRPPAKIWNAIVPLYPNPIAGIEDIDTSTH
                     FTYSEGQPNSRLVEEVPVFLAGIRR"
BASE COUNT          383 a          832 c          809 g          418 t
ORIGIN      
        1 ccgtcgcgtc atccaaaccg ttgcgcgacc gacggttgtg tttaggttcc ccgcaccgac
       61 caaggaggtt gccgatgaat gcgattcgac gactggtagt tgcgacgctg ttggcggtgt
      121 ggtgcttccc ggccgtaagc agcgccaatc cctcgcccgc ggctcctgcc ccgatcgcgg
      181 cggaaccggc ctcgccgatc ccggcgacct ccgtcgagaa caacgaaacc gagatcggca
      241 gcctcgccgc ccgccagcag caggcgcgcc agctcgagga cttcaaagga ggccgggcct
      301 acatcttggg cagcagcgtc gtggtcgtcc tgctcgtcgt cctgctggtg ctgctcctcg
      361 tgtgacgctt ttcctgcgtc gggcttgcgc gcactcgacg gacgtctaac gcgaccgatg
      421 tggcgcctcg agcagatcgg acaagaacac cgcgggcgga cgtccgccgg aggttgaatg
      481 catcagcttc ttttcaacgg acaggtagag gcacagcggt gttcgatcgt gctgcggcat
      541 ccgggccacc agctcggcga cggcgggttc gggcgagtcg gcgtccccgg ccacgcacac
      601 gtaatgggcg tcgaggtatt cgtggatctc ttccttcgcg atcgtccgct ccaccagcgc
      661 gcgggtgcca ccgcaggatt gtcggccgac gaccagcagc accgcccgct tggattcgcc
      721 ggcggcgacc agcccctcgg cgaggctcgc gtgccaggca actggcgaga cgaaatgcgg
      781 atgtcggccg gcctcagcca tttcaccttc ctatcacgag ttgattcgcc gtcactttgc
      841 tagggttgcg caatggcggc gcggcgactg gcgaggatgg cccgacagct ccccctcggg
      901 cccgagggga cgttgcggct cgcggtcgtg gcggacaccc actcgcagcc tcacccgcgg
      961 gcagccgagt acctggctgc ccgggcgccc gatgccatac tgcacgcagg ggacatcgga
     1021 gatctggccg tgctcgatca gctctcgtcc atcgcccccg tgcttgcgat tcgcggcaac
     1081 atcgacgtcc acgcgcccga cttgccggac gtgctcacgc tcgatctcgt gcgcagcgtc
     1141 gaacatccgc aaccggtgct ccggatgctg ctcgttcaca tcggcgtcta cggcccgaag
     1201 ctgcgcgcgg aggtcgcggc gacggcacgt gcgcagggag cgcaggccgt ggtgtgcggg
     1261 cactcgcacg tgccatttct cgggcgcgac cgtgagctta cggtcttcaa ccccggatcg
     1321 atcgggccgc ggcgttttca cctacccatc gtcttcggca cgatcgacgt gactcccgca
     1381 ggcttgcggt tcgcgcacgt ggactgcgag accggagcgc agtggacccc gccatagggt
     1441 tcacgctccg cgtcgtgcgg caccggcgtc ttgacaggtg cccggagtga aggagcagaa
     1501 gacctccaga tggtcagggt gacagcacgc ccgaactggt cgtctgcttc ttgcctcatc
     1561 gaatcaagtg ctcgtcgggg cgcgcagacg cagtcagatc agctcggcga gtccctcgct
     1621 cggccacccg acgctcgggg tgtgcccggc cggtcgcggt gccgctctcg ccatgatcgg
     1681 cggcgagatc cgcgtcaggg ggagccggaa ccgcccgcga ccggcaccaa gtggtcctcg
     1741 tcgatgatca ggccgcggca gttgtcggcg ccgcagcggc acggctcagc caggtgggcc
     1801 gggaatgcgt agtcatagga cagctcctcg ccggccgcga tcggccgcaa ggcgatgacg
     1861 ctggcccagg cctcgccgtc gtccgtcacg cccacgtcaa cccaggtgtt ggggtcgcac
     1921 gagtggttga tgaaccgcgt ctggtccacc atgtcgaaat agaccccgtt ctcgatccgc
     1981 agcgagtagc ggtcgtcgac gttctcgcct tcccgccagg cgaccccgtc gacctcagcg
     2041 atcgcttcac cttcggcgaa gtccctcagg gcgacgaccc cgtatccgtg aatgggtgag
     2101 cggaccacgc gaatcccggt cactctcggc atcgttcacc gattgtactc agggtggagg
     2161 gtaagagttc aaccggacgg cgagctgcgc tgcggcatgc tcccggggca ggtggccgtt
     2221 cagtcgcttg ggttgtgcag gcgacggccg ccgcgacgag attctcggcc gggtccgtac
     2281 ggccgccggc gaagatctgg aatgcaatcg tcccgctgta tccgaacccg atcgccggga
     2341 tcgaggacat cgacaccagc actcacttta cgtacagcga ggggcaaccg aactcgcggc
     2401 tcgtcgaaga ggtgccggtg ttcctggccg gaatccgacg ac
//
LOCUS       DASWVF010000031         5388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_281107, whole genome shotgun sequence.
ACCESSION   DASWVF010000031 DASWVF010000000
VERSION     DASWVF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281107"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VGD55_00520"
     CDS             complement(<1..514)
                     /locus_tag="VGD55_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868857.1"
                     /translation="MSSSGRFAQPNLAAGVVAGLLAGVVGAILWGGFTDLTHFRIGYL
                     ALGIGVLVGYAIVRVGQVRTVAVGVTAAVITLVACAAGDTGSVYFQASHDLHQSVGTL
                     LSVSNPFTVFRVDMQHNAFGLVFFAIAAWAAFRYVTTGRGLMRPRRPVQVPPGAGYGT
                     QPSYGQPSASA"
     gene            577..1623
                     /locus_tag="VGD55_00525"
     CDS             577..1623
                     /locus_tag="VGD55_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0868858.1"
                     /translation="MPTPSRPLLPELPWPPGSATGIGSLPGVDPDEATRIVVGELSAF
                     PHLVELPAAGVGADMVGRGAAHLVGLHVDVQPSGWRFAAHEGSEERRARSMLARDLDV
                     LEEHTQGFIGPLKIQVAGPWTLASSLELRYGDKALADPGAVRDITAALAEGIAEVARD
                     VARRVPQAVVVVQIDEPLLPAVLVGRVPTASGFGTLRVVELNLAIDRLRTVVDMVTSA
                     GAIPIAHCFAAAPPVSLFAEAGFRAVSLDATLLTERDDDAIGETVEAGLRFFLGLVPS
                     MDVDPLPRMTELASPARRLWSRLGFAPEDLARNVVVTPTCGLAGASPSWPKMAYHASL
                     EVARVLLEAPEEDS"
     gene            1620..3707
                     /gene="ligA"
                     /locus_tag="VGD55_00530"
     CDS             1620..3707
                     /gene="ligA"
                     /locus_tag="VGD55_00530"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0868859.1"
                     /translation="MSTDADVPVDVAERHAVLSRELDDHAYRYYVLDSPIVSDAEYDT
                     AMRELKSIEESYPQLRTPNSPTQKVAGSYSTQFTPVTHLDRLLSLDNVFSVEELHAWA
                     ARTERDAGEPVEYLCELKVDGLAVDLVYEQGKLVRGATRGDGYTGEDITPNIRTLANV
                     PHTLKPAADWPVPDLLEVRGEVYFPVAGFADLNASLVEAGKAPFANPRNTAAGSLRQK
                     DPRVTASRSLRMVVHGVGAVKGFAPLRQSQAYEAFAAWGLPTSDRVRVLSTLAEVDDF
                     IAHYGEHRHDVEHEIDGVVVKVDQISLQRRLGTTSRAPRWAIAYKYPPEEVTTKLLAI
                     EVNVGRTGRITPFGVMQPVKVSGSTVERATLHNEDEIKRKGVRVGDTIVLRKAGDVIP
                     EIVGPVVDLRDGTEYEFVFPTSCPACGTALSRPAGEADTRCPNQRSCPAQLRERLFHL
                     AGRGAFDIEVLGYEAAVALLDSKLIVDEGDLFSLDADQLARVPFFTKQDGELSANAKK
                     LIENLEAAKTRPLWRVLVALSIRHVGPTAAQALAREFGSIEAIRAASASDLASADGVG
                     MTIAQSIGEWFEVDWHAEIVRKWGAAGVAMAEVREDEGPRPLAGLTVVVTGSLTGWSR
                     DSATEAIQNAGGKVVGSVSKKTDFVVVGDNPGSKYDKALTLGVPVLDESGFQILLDDG
                     PDAARLGLLVEPD"
     gene            3818..5128
                     /locus_tag="VGD55_00535"
     CDS             3818..5128
                     /locus_tag="VGD55_00535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0868860.1"
                     /translation="MSEPSDRRNLRPPPGSTLWWYLWSVIVLAVLIVAICVTGLHHGD
                     ISALLRRPAFWLVAAPMAVTALRPIAPKGRSGDGTYALVVFLFALLLRVGLPAAVALC
                     TITMLVRGALYKQALHRNLFNAAQHIVTLAASWLVLQMFGIDATPLHPWAFTEPGLRI
                     SELLAVGLAGLAYLVVNNGSVYIAMSIVEGRSILSIARDEARQLMIVGIAMVSLSPLV
                     LVVMVHDWPLVPLFYPALISLYRNAASSVAHEHDALHDWLTGLGNRELLHREATRALD
                     APPRPGGGIAVLVLDVDRFKSVNDTLGHAAGDRLLQIVAERLMAVVRPADVLARLGGD
                     EFVVLLHEVADLATARMAAVRLLDRVNGQCEIDGTLIDLRVSVGVALAPDHGREFDGL
                     LRRADGAMYVAKAAGCGVALFDSNVDDDRRPSPDVAREIARPKQ"
     gene            5202..>5388
                     /locus_tag="VGD55_00540"
     CDS             5202..>5388
                     /locus_tag="VGD55_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868861.1"
                     /translation="MFGQNKHLEKKLREHGLSTSAEILVVHATNMAITHGNDSIISNT
                     EIVVKLTVRLTPPGEAPW"
BASE COUNT          889 a         1763 c         1776 g          960 t
ORIGIN      
        1 gagcggacgc cgagggctga ccgtatgacg gttgggtgcc atatcccgcg cccgggggta
       61 cctgaaccgg ccggcgcggc cgcatcagcc cgcgcccggt ggtgacgtag cgaaatgcgg
      121 cccaggcggc gatcgcaaag aacaccaggc cgaacgcgtt gtgctgcatg tccacgcgga
      181 acacggtgaa gggattcgag accgacaata aggtgccgac ggactggtgc aagtcgtgcg
      241 aagcctggaa gtagaccgag ccggtatcgc ccgcagcgca cgccaccaag gtgatgaccg
      301 cggcggtcac tccgacggcg accgtgcgta cttggccgac ccgcacaatc gcgtacccca
      361 ccaggacgcc gatcccaagt gccaggtacc cgatccgaaa gtgcgtgaga tcggtgaacc
      421 cgccccacag gatcgcgccg acgacgcccg caagtaagcc tgcgaccaca cctgcggcca
      481 gattcggttg tgcaaacctc ccagaactgc tcattccacg aatgtagccc cttggtcgtc
      541 ggtcgttatg tcgttctcga ttactaggct cctcttgtgc cgacgccgtc ccgacccttg
      601 ttgcctgagc tgccgtggcc gccggggtca gctaccggca tcgggtcgtt gcccggcgtc
      661 gatcccgacg aggcgacccg catcgtcgtc ggcgaactgt cagcgtttcc ccatctggtc
      721 gaattgccgg cggccggcgt gggtgccgac atggtcggtc gcggcgcggc acatctggtc
      781 ggcctgcacg tcgatgtcca gccatccggc tggcgattcg ccgcacacga gggctccgaa
      841 gaacgacgtg cgcgcagcat gctggcccgc gacctcgacg tcctcgaaga acacacgcaa
      901 ggattcatcg gtccgctgaa gatccaggtc gctggcccgt ggaccctcgc gtcctccctc
      961 gaattgcgct acggagacaa ggcgctggcc gatccgggcg ccgtccgcga tatcaccgcc
     1021 gcactcgctg aagggatcgc ggaggttgca cgcgatgttg cgcgcagggt gccacaagcg
     1081 gtcgtcgtcg ttcagattga cgagccgctg ctgccggccg tgcttgtcgg cagggttcca
     1141 accgccagtg gcttcggtac cttgcgcgtc gtcgagttga acctcgcgat cgacaggctg
     1201 cgcacggtcg tcgacatggt gacgtcggcc ggtgcgattc ctatcgcgca ctgtttcgcc
     1261 gctgcgccac cggtcagctt gttcgccgaa gcaggctttc gagcggtcag tcttgacgca
     1321 acgctgctga ccgagcgcga cgacgatgcg atcggcgaga ccgtcgaggc tgggcttcga
     1381 ttcttccttg gtctcgttcc ttcgatggac gtcgatccgt tgccgcgcat gactgagctc
     1441 gcatcgcccg cgcgccgctt gtggtcgcga ctcggattcg caccagaaga cctggcacgc
     1501 aacgttgtcg tgacaccgac ctgtggtctt gcaggcgcgt cgccctcatg gcccaaaatg
     1561 gcttaccacg caagccttga ggtggcgcgg gtgctactcg aggcacccga ggaggactcg
     1621 tgagtaccga tgccgacgtg ccggtcgacg tcgccgaacg gcacgccgtc ctgtcccgcg
     1681 aactcgatga ccatgcgtat cgctattacg tcctcgactc gccgatcgtg tctgatgccg
     1741 agtacgacac ggcgatgcgc gagctcaagt cgatcgagga gtcctacccg cagctgcgca
     1801 cccccaattc accgacgcag aaggtcgcgg gcagctactc gacccagttc acgccggtca
     1861 cccaccttga ccggctgctg agtctcgaca acgtcttctc cgtcgaagaa ctgcacgcgt
     1921 gggccgctcg caccgaacgc gatgccggcg agccagtcga gtacttgtgt gagctgaagg
     1981 tcgacgggct agcggtcgac ctcgtctacg aacagggaaa gctggtgcgc ggcgcgaccc
     2041 gcggcgacgg atacaccggc gaagacatca ccccgaatat ccgcacgctc gcgaacgtgc
     2101 cgcacacgtt gaagcctgcg gccgactggc cggtacccga cctgctcgaa gttcgcggtg
     2161 aggtctactt ccccgtcgcc ggatttgccg acctcaatgc ctctctcgtc gaggcgggca
     2221 aggcgccgtt tgcaaacccg cgcaacactg ctgccgggtc gttgcggcag aaggatccgc
     2281 gggtgacggc gagccgctcg ctgcgcatgg tcgtgcacgg ggtgggtgcg gtaaagggct
     2341 tcgcgccgtt gcgccaatcg caagcctacg aagcattcgc ggcctggggc ctgccgacga
     2401 gcgaccgcgt gcgcgtgctg tcaacccttg cggaggtcga cgacttcatc gcgcactacg
     2461 gcgagcaccg ccacgacgtc gagcacgaga tcgacggtgt agtggtcaag gtcgatcaga
     2521 tctcgttgca acgccggctc ggcaccacgt cgagggcgcc gcgttgggcg atcgcgtaca
     2581 agtacccgcc cgaggaggtc acgaccaagc tgctcgcgat cgaagtcaac gtcggtcgca
     2641 ccggtcgcat caccccgttc ggcgtcatgc agcccgtgaa ggtgtccggc tcgaccgtcg
     2701 agcgggcaac cctgcacaac gaagacgaga tcaagcgcaa gggcgttcgg gtaggcgaca
     2761 cgatcgtgtt gcgaaaggcc ggcgacgtca tccccgagat cgtcggtccc gtcgtcgacc
     2821 tgcgcgacgg caccgagtac gagttcgtct ttccgaccag ctgcccggcg tgcggcacag
     2881 cgttgtctcg acctgccggt gaggctgata cccggtgtcc gaaccaacgc tcgtgcccgg
     2941 ctcagctgcg cgagcgactc ttccacctgg ccgggcgcgg cgcgttcgac atcgaggtgc
     3001 tcggctacga ggccgcggtg gctttgctcg attcgaagct catcgtcgat gagggcgacc
     3061 tcttttcttt ggacgccgac cagctggcgc gagtgccgtt cttcaccaag caggacggcg
     3121 agctctcggc gaacgcgaag aagttgatcg agaacctcga agcggccaag actcggccgc
     3181 tgtggcgggt gcttgtcgcg ctgtcgatac ggcacgtcgg cccgacggcc gcccaagcgc
     3241 tcgcccgtga gttcggatcg atcgaagcga ttcgcgcggc gtcggcgtcc gacctggctt
     3301 cggccgatgg ggttggcatg acgatcgcgc agtcgatcgg cgagtggttc gaagttgact
     3361 ggcacgccga gatcgtgcgc aagtggggag ccgctggcgt ggcgatggcg gaggtgcggg
     3421 aggacgaagg tccgcgtccc ctcgccggtc tgacagtcgt ggtcaccggc tcgttgacgg
     3481 ggtggagccg tgactcggcc accgaggcga tccagaacgc cggcggcaag gtcgtgggct
     3541 cggtttccaa gaagaccgac ttcgtggttg tcggcgacaa cccgggctcg aagtacgaca
     3601 aggcgctgac cctcggcgtc cccgtgcttg atgaaagtgg gtttcagatc ttgctcgacg
     3661 acgggcctga cgccgcccgc ttgggcctgc tggtcgagcc tgactgagcc acctgcgcta
     3721 tccgttaacg cacgggtgcc gtcgatggat caggtatccg aacaactcgc gcgacatggc
     3781 gcaacctggc ccacacccag gccgggccga ggaagtcatg agcgaaccgt ccgaccgtcg
     3841 caatctgcgg ccgccgcccg gctcgacgct gtggtggtac ctgtggtcgg tcattgtgct
     3901 ggcggtcttg atcgtcgcca tttgcgtcac cggcctgcac cacggtgaca tctcggcgct
     3961 gctgcgacgg cccgcgttct ggctagtcgc agctccgatg gcggtcaccg cactgcggcc
     4021 gattgccccg aaagggcgca gtggcgacgg tacgtatgcc ctcgtcgtct ttctgttcgc
     4081 gttgctgttg cgggtaggtc taccggccgc tgttgctctc tgcacgatca cgatgctcgt
     4141 ccgaggcgcg ctctacaagc aagcgctgca ccgcaacctg ttcaacgccg cccagcacat
     4201 cgtcacgctc gcggcttcgt ggttggtcct gcaaatgttc gggatcgacg ccacaccttt
     4261 gcatccctgg gcgttcaccg aaccgggcct gcggatcagc gagctcctgg cggttggcct
     4321 tgccggcctt gcctacctgg tcgtcaacaa cggcagcgtc tacatcgcca tgtcgatcgt
     4381 cgagggtcgg tcgatccttt ctatcgctcg cgacgaagcg cgccagctca tgatcgttgg
     4441 tattgcgatg gtgagcttgt cgccactcgt cctggtcgtc atggtgcacg actggccgct
     4501 cgtgccgttg ttctatcccg cgcttatctc gttgtatcgc aacgctgcat cgtccgtcgc
     4561 gcatgagcac gacgcgttgc acgactggct caccggcctt ggcaacaggg agctgctgca
     4621 ccgtgaggcg acgcgagcgc tcgacgcgcc gcctcgcccg ggcgggggta tcgcggtctt
     4681 ggtgctcgac gtcgacaggt tcaagagcgt caacgacacg ctcggccacg cggccggtga
     4741 ccggctcttg cagattgtcg ccgagcgact gatggctgtt gtgcggcctg ccgacgtcct
     4801 ggcgcggctc gggggcgacg agttcgtcgt gctgctgcac gaggttgccg atctcgccac
     4861 cgcgcgcatg gccgccgtcc gccttcttga tcgggtgaac gggcagtgcg agatcgacgg
     4921 caccttgatc gacttgcgag tcagcgtcgg agttgccctg gcgcccgatc acgggcgtga
     4981 attcgacggt ctgttgcggc gcgccgacgg ggccatgtac gtcgcgaagg cagccggttg
     5041 cggggtggca ctgtttgatt cgaacgtcga cgacgaccgg cgcccgagtc cggacgtcgc
     5101 ccgcgagatt gcacgcccga agcaataagt cgatgctgga gtcgggcgcg tctcgcatcc
     5161 gcggggatcc agcggtcagc agacagcgac gggcggcgac gatgttcgga cagaacaagc
     5221 acctggagaa gaagctccgc gagcacggcc tgagtacgag cgccgaaatc ctcgtggtcc
     5281 acgccaccaa catggcgatc acgcatggca acgattcgat tatcagcaac accgaaatcg
     5341 tcgtgaagct gaccgtgcgg ctgacgccgc caggcgaggc tccatggg
//
LOCUS       DASWVF010000032         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_51870, whole genome shotgun sequence.
ACCESSION   DASWVF010000032 DASWVF010000000
VERSION     DASWVF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51870"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGD55_00545"
     CDS             complement(<1..424)
                     /locus_tag="VGD55_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY0868862.1"
                     /translation="MARVPPDEMIRLERDISLERLATARGVNLTKSGAALTGTCPFHP
                     SSKRPPTITIGPKSNTWACSKCSVTDGTVVLWTMKAEGISRSHALELLRADHGVGGGK
                     VVKSSTVTKLPDVTANAADDTVLLGQVAAYYADALTRNV"
     gene            complement(513..845)
                     /locus_tag="VGD55_00550"
     CDS             complement(513..845)
                     /locus_tag="VGD55_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868863.1"
                     /translation="MSLDRRWWRSKLDEGAQFQCRVEPSQLLQEGESVSRLPKRKCGS
                     MNPSHEIIPAPLACESGPTAPATALCFRTSTLKAAAAEGTGPLGGPRAGDNASGHEGI
                     DDDDGRRM"
     gene            complement(835..1836)
                     /locus_tag="VGD55_00555"
     CDS             complement(835..1836)
                     /locus_tag="VGD55_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868864.1"
                     /translation="MRVGLSKLEARVETATKQLARLEGGTEKFGPMLTSVMREGIPLE
                     VFTRMALADSGYKVHGRYLYKFYNSDGWIERSVDVYATQERTFTVPPDGGRQAGESWP
                     ERDHLLVEAKQRRNGVTWIFCHLPSSEKNHHFAPRDVPHVNFGYELRPGEHGPKPTGN
                     TKDVENGLAQLEYAYIPKMVDVHNQWKTDHQHLGPRVFRGNGVRRDSTWALLVTNARL
                     MLFKPPATLAELEQDPPPRDDRLFVEAPWLVYQSQPSLHLLGHQATHVGPLRGIPEGD
                     DIVLPVLMADQSCQVHIVNYNHLPRFLRLVEDPPRIKKIVVQLSVDGKESPPFKIEP"
     gene            2096..2407
                     /locus_tag="VGD55_00560"
     CDS             2096..2407
                     /locus_tag="VGD55_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868865.1"
                     /translation="MYWTLTRETTVARFGHDEGGHWIEVTDGMIVARHDATEHGFDGD
                     RPVLSILTVLCEYGFIADDDVREALARLDGRPDSGRRPSGPGVRRVLRIIERLRLASG
                     E"
BASE COUNT          410 a          784 c          797 g          506 t
ORIGIN      
        1 ttacgttcct cgtgagcgcg tcggcgtagt aggcggccac ctgcccgagc agcacggtgt
       61 cgtcggcggc gttggcggtc acgtccggca acttcgtgac cgtcgaggac tttacgacct
      121 tgccgccgcc gacgccgtga tcggcccgaa gaagttcgag tgcgtggctg cgggagatcc
      181 cttcggcctt catcgtccac agcacgacgg tgccgtccgt cacgctgcac ttcgagcacg
      241 cccacgtgtt cgacttcggg ccgatcgtga tcgttggcgg cctcttcgag gagggatgga
      301 aggggcaggt gccggtgagg gcagcgccgg acttggtaag gttcacgcct cgtgctgtgg
      361 cgagccgttc gagagagatg tcccgttcga gccggatcat ctcgtcgggt ggtacgcggg
      421 ccatccgaac agctacccgc cgcccccctc acgatcaacg aggacagggt ttgatcatcc
      481 ccgggccccc gtgtagcggg cgcatggacc cgtcacatgc gcctgccgtc gtcgtcgtcg
      541 atgccttcgt gcccgctggc attgtcgcca gccctgggac caccgagcgg acctgtgccc
      601 tctgcggcgg cagccttcag ggtcgacgtc cggaagcaca gtgctgtggc gggcgctgtc
      661 gggccgctct cgcacgccag cggcgcaggg atgatctcgt ggctcgggtt catcgagccg
      721 cacttgcgct tcgggaggcg gctgacgctt tcgccttctt gaaggagctg gctgggctcg
      781 acgcgacact ggaactgggc gccctcgtcg agcttcgagc gccaccagcg gcgatcaagg
      841 ctcaatcttg aatggtggcg attctttccc atcgacgctt agttggacca cgattttctt
      901 gattctcggt ggatcctcta cgagtctgag aaacctaggc aagtggttgt agttcacgat
      961 atgtacttgg cacgactggt cggccatgag gaccggaagc acaatatcgt cgccttcggg
     1021 gatacctcga agcggcccta catgagtcgc ctggtgccct agcaggtgaa gcgagggctg
     1081 gctttggtac actagccacg gcgcctcgac gaacagtcga tcgtcgcgcg gcgggggatc
     1141 ttgctccagc tcggccagcg ttgcaggcgg tttgaacagc atgagacggg cgttcgtgac
     1201 gaggagcgcc cacgtcgagt cgcggcgcac cccattcccg cgaaagactc tcggtcccaa
     1261 gtgctggtga tctgttttcc actgattatg gacgtcaacc atcttcggga tatacgcata
     1321 ctcaagctgt gccagcccgt tctccacgtc cttcgtgttg cccgttggtt tgggaccgtg
     1381 ttctcccggc cgcagttcgt aaccgaagtt cacgtgcggg acgtcgcgtg gcgcaaagtg
     1441 gtggttcttc tccgaagatg gcaggtgaca gaaaatccaa gtcacgccgt tgcgccgctg
     1501 tttcgcctca accaagagat gatctctctc tggccatgat tctccggctt ggcgtccgcc
     1561 gtctggcgga acggtgaacg ttcgctcctg cgttgcgtac acgtcgaccg accgctcgat
     1621 ccagccatcc gagttgtaga acttgtacag gtagcgaccg tggaccttgt accccgagtc
     1681 cgccaacgcc atccgcgtga atacctcaag tggtattccc tcgcgcatga ccgacgtcag
     1741 catcgggccg aacttctcgg tgccaccttc gagtctcgcg agctgcttcg tggcggtctc
     1801 gacgcgcgct tcgagcttcg agagtccgac gcgcaaggct tcgctccgtt gcgccgcatc
     1861 ggaaagcgcg gcccgaatct ccttcatgaa cggaacttgg tcgatgaagc tcttcaacat
     1921 gatgccccca ccggagccgg tatgcgcgac atcgtacccc tttgcgttcg ttcaggggca
     1981 gcgtcgagct gcacggtact ggggccgcct tgtgcgcaac ccgcgacgac ccttgggacc
     2041 agcacggcgg tcctggcgcc attgatcgtc ccgcgctgcc agggtactcg ccaaggtgta
     2101 ctggacgctc acgcgagaaa ccacggtagc ccgcttcggc cacgacgagg gtggccactg
     2161 gatcgaggtg acggacggca tgatcgtcgc ccgccacgac gcgaccgagc acgggttcga
     2221 cggggaccga cccgtgctgt caatcctgac cgtcctgtgc gagtacggat tcatcgcgga
     2281 cgatgacgtg cgcgaggccc tcgcgcggct cgacggacgt cctgattcgg gtcgacgtcc
     2341 atccggaccc ggggtgagac gggtgctgcg gatcatcgag cggctgcggc tggcgtcggg
     2401 ggagtgaggt gacgaaggag tggtcgaact tgagctacgc catctctgct tttcttgacg
     2461 ctgcagaacg ctactatccc caactgattc ttcagat
//
LOCUS       DASWVF010000033         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_484950, whole genome shotgun sequence.
ACCESSION   DASWVF010000033 DASWVF010000000
VERSION     DASWVF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484950"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..2787
                     /gene="leuS"
                     /locus_tag="VGD55_00565"
     CDS             325..2787
                     /gene="leuS"
                     /locus_tag="VGD55_00565"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0868866.1"
                     /translation="MSETFDFAAIQDRWLPVWDALDPFRTGRPGPDGAPRERRYMLDM
                     FPYPSGDLHMGHAEAYAFGDVVARYWQQRGYDVLHPIGWDAFGLPAENAAIARNQHPA
                     DWTYKNIETQAASFRRYAVSFDWSRRLQTCDPQYYHWTQWLFLRFFERGLAYRKASAV
                     NWCPNDQTVLANEQVVNGHCERCGTLVTKKTLTQWYFKITEYADRLLDDMSALEGSWP
                     ERVLTMQRNWIGRSQGAHVDFAIEGRAEPVTVFTTRPDTLYGATFFVVAADSPLAASL
                     CAPEQRAAFDAYVEQVRRLSDIDRMSTERAKTGVFLGRYAINPVNGERIPVWAADYVL
                     ADYGTGAIMAVPAHDQRDLDFARTFDLPVRVVVATDQPDPAETGVATPGDGVHVNSGS
                     LDGTEKEAAISQIVAELEAKGVGKGAINYRLRDWLLSRQRFWGAPSPIVHCPVDGEVP
                     VPDDELPVLLPDLRGADLQPKGVSPLAAATEWVNTTCPKCGGPALRDTDTMDTFVDSS
                     WYFLRYCSPHRDDVVFDPDDVKQWMPVDQYVGGVEHAILHLLYSRFFVKVLHDMGLIE
                     FDEPFKALLNQGQVINGGKAMSKSLGNGVDLGEQLAAFGVDAVRMTMVFAGPPEEDID
                     WADMNVPALGKFLARAWRLAGEVTSAVGVDVTIGDLALRRVTHHTVHDVTHLLDHFRF
                     NVAIARIMELVNAIRKTVDVGPGAGDPAVREAVEAVAVMLSLFAPYTAEDAWSRLGHQ
                     PTVFHAGWPTVDPSLLIEESVTCVVQINGKVRDRLVVAPSVEEVALRELALSAPAVVK
                     ALDGRAVRTVIVRAPKLVNIVV"
     gene            2794..>3420
                     /locus_tag="VGD55_00570"
     CDS             2794..>3420
                     /locus_tag="VGD55_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY0868867.1"
                     /translation="MPRARKGTPTVAVVTDSTAYLPPGYAQRLGVQVVPLQVIVGGVV
                     RAEGEEIAPSEVAAVLHRGHLVSTSRPSPSLFAAAYHRAFAGGASSIVSVQLSGAMSG
                     TVDAARLAAADVGGDITVIDSRSVGMGMGFAVIAACEAASAGADASGVIDTVTRTLAA
                     TEAIFYVDTLEFLRRGGRIGAARAVVGSALGVKPLLCLRDGRIEPLEQV"
BASE COUNT          543 a         1126 c         1138 g          613 t
ORIGIN      
        1 cccccaagct ggcgacctac cgactaaaag tttgcgcgaa tggagccccg gtcggtacca
       61 gccatccggt tagaagacgc tgagcatctg ccgagtccgc gcaggcgttt ggcgcattca
      121 ggtgaccatt gggacatatc cgcccagatg ccctgagttc gccctaagct gggtggtcac
      181 cgcgacggcg ccccgggcgt acaatcgccc gcatgtcgca gctgcttctt gccctgccgc
      241 gctgacacca gtcggcgcgg cgacccctcg tgcgcgaggg gtctttttgt gtcaactgca
      301 cgacccactc gacgtgagga accgatgagc gagacgttcg acttcgcggc catccaggat
      361 aggtggctgc cggtctggga cgccctcgac ccgttccgca ccggtcggcc cggccctgac
      421 ggcgcaccgc gcgagcgccg ctacatgctc gacatgtttc cttatccgtc gggcgacctg
      481 cacatgggtc acgcggaggc atacgcgttc ggcgacgtcg tcgcgcgcta ctggcagcag
      541 cgcgggtacg acgtgctgca cccgatcggg tgggacgcgt tcgggttgcc ggccgagaac
      601 gcggctatcg cgcgcaacca gcatccggct gactggacct acaagaacat cgagacccag
      661 gcggcctcgt tccggcggta cgcagtctcg ttcgactggt cgcgtcggct ccagacctgc
      721 gacccgcagt actaccactg gacccagtgg ttgttcctgc gcttcttcga gcgcggactc
      781 gcgtaccgca aggcgtcggc ggtgaactgg tgtccaaacg accagaccgt gttggcgaac
      841 gagcaggtcg tcaacggtca ctgcgagcgc tgcggcacgc tggtcaccaa gaagaccctg
      901 acgcagtggt acttcaagat caccgagtac gccgaccggc tgctcgacga catgagcgcg
      961 ctcgagggca gctggcccga gcgggtgctg acgatgcagc gcaactggat cggccgctcg
     1021 cagggcgcgc acgtcgactt cgcgatcgaa ggccgcgccg agccggtcac cgttttcacc
     1081 acgcgccccg acacgttgta cggcgctacg ttcttcgtcg tcgcggccga ctcgccgctg
     1141 gccgcctcgc tgtgcgcccc tgaacagcgc gctgccttcg atgcttatgt cgagcaggtc
     1201 agacggctat ccgacatcga ccgcatgtcg accgagcgcg ccaagaccgg cgtgttcctc
     1261 ggccggtacg cgatcaaccc cgtgaatggt gagcggatac cggtgtgggc cgccgactat
     1321 gtgctggccg actacggcac cggcgccatc atggcggtgc cggcgcacga tcagcgcgac
     1381 ctcgatttcg cgcgcacatt cgacctcccg gtgcgggtgg tcgtcgctac tgatcagccc
     1441 gatcctgccg agactggcgt cgcgacgcca ggtgacggcg tccatgtgaa ttctggttcg
     1501 ctcgacggca ccgagaagga ggcggcaatc tcgcagatcg tcgctgaact cgaggcgaag
     1561 ggtgtcggca agggcgcgat caactatcgg ttgcgcgatt ggttgttgtc acggcagcga
     1621 ttctggggcg cgccgagtcc gatcgtgcac tgccccgtcg acggcgaagt tccggtgccg
     1681 gacgacgagt tgccggtgtt attgcccgac ctgcgcggcg ctgacttgca gccgaagggc
     1741 gtctcaccac tggccgccgc gaccgagtgg gtgaacacca cgtgccccaa atgcggcggt
     1801 cctgcgctgc gcgataccga cacgatggac acgttcgtcg actcgtcgtg gtacttcctg
     1861 cggtactgct caccgcatcg tgacgacgtc gtattcgatc ccgacgatgt gaagcagtgg
     1921 atgcccgtcg accagtatgt cggcggcgtc gaacacgcga tcctgcatct gctgtacagc
     1981 aggttcttcg tcaaggtgct gcacgacatg ggactcatcg agttcgacga accgttcaag
     2041 gcattgctga atcaaggcca ggtcatcaac ggcggcaaag caatgagcaa gtcgcttggc
     2101 aacggcgtcg acctgggtga acaactcgcc gcgttcggcg tcgacgccgt acggatgacg
     2161 atggtgttcg ccggcccacc cgaggaagac atcgactggg ccgatatgaa cgtgcccgcg
     2221 ctagggaaat tcctcgcgcg cgcgtggcgg ttggcgggcg aagtgaccag cgctgtcggg
     2281 gttgatgtca cgatcggcga tctcgcgctg cggcgcgtga cgcaccacac cgtgcacgac
     2341 gtcacgcacc tgctcgacca cttccggttc aacgtcgcga tcgcgcggat catggaactt
     2401 gtgaatgcga tccgcaagac cgtcgacgtc ggtcccggcg cgggcgaccc tgcggttcgg
     2461 gaagcggtcg aggcggtcgc cgtcatgctg tcgctgttcg cgccctacac ggcagaagac
     2521 gcgtggtcac gcctcggaca tcagccgacg gttttccacg ccggctggcc caccgtcgat
     2581 ccttcgctgc tgatcgagga gtcggtcacc tgtgtggtgc agatcaacgg caaggtgcgg
     2641 gaccggctcg tcgtcgcgcc ttctgtcgaa gaggtcgcgc tgcgcgagct cgcactctcc
     2701 gcgccggcgg tcgtgaaggc actcgacgga cgcgctgtgc gcacggtcat cgtgcgcgct
     2761 cccaaactcg tgaacatcgt ggtctaatcc ctcatgccgc gagcacgcaa ggggacgccg
     2821 acggtggctg ttgtcaccga ctccactgct tacctgccgc ccggatatgc ccagcgcctc
     2881 ggcgtccagg tggtgccctt gcaggtcatc gtcggcggcg tcgtgcgagc cgagggcgag
     2941 gaaattgcgc cgagcgaggt cgccgcggtc ttgcatcgcg ggcacctggt gagcacgtcg
     3001 cggccatcgc cgagtctgtt cgccgccgcg tatcaccgag cgtttgcggg cggcgcttcg
     3061 tcgatcgtgt cggtgcagtt gtcgggcgcg atgtcgggca ccgtcgacgc cgctcggctg
     3121 gctgcagccg atgtcggcgg cgacatcacc gtcatcgatt cgcgctcggt cggcatgggc
     3181 atgggcttcg cggtgattgc cgcctgcgaa gctgcatctg ctggcgccga cgcgtcgggc
     3241 gtcatcgaca ccgtcacgcg cacgctcgcc gcgaccgagg cgatcttcta cgtcgacacg
     3301 cttgagttct tgcgtcgcgg tggccgcatc ggcgcggccc gcgcggtggt cggatctgcg
     3361 ctcggcgtga agccgctgct gtgcttgcgt gacgggcgca tcgaacctct cgaacaggtg
//
LOCUS       DASWVF010000034         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_306632, whole genome shotgun sequence.
ACCESSION   DASWVF010000034 DASWVF010000000
VERSION     DASWVF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306632"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGD55_00575"
     CDS             <1..902
                     /locus_tag="VGD55_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0868868.1"
                     /translation="AHSAVFFVQTIPRLARTHDIRAAPQRDALLVAHAITSEALERAR
                     TNGWSVVTDDGAGWVRLAERTLHFETTTAADAPKRPPGRPGRGVFSVVRTLFTMDEDG
                     AHQQEIAEFAHIRQAAVSKALNRLAQLDLVARGPNGWQVINRAGAISWWLENYPGPGG
                     IETHWFGVDSIREQAYDAYAALDQMHARPLISGDVAADLVAPWRTPRRAVLYAKRGAD
                     LSSVGLTPSYAPTATLTLVLPQDLGVWPLPKAPALIEMQGHHVARANAFQVLFDLARS
                     PGPDVSEALEAWRTWMVQPGSLL"
     gene            863..>1541
                     /locus_tag="VGD55_00580"
     CDS             863..>1541
                     /locus_tag="VGD55_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868869.1"
                     /translation="MANVDGPTRLVVVTELRLHSTSRAEDAGFLAMRDIAAVADETAI
                     GYRIIGGQMVRLHVALAGVTEPTVRVTQDADMGIEAASARNPALVAGLEVLGYTRPEA
                     SNRFIRATESGLSLVIDVLTPAWGRRMVSNQRHGEMTVDEIPGLSLALAGTGENIDLA
                     VTLLDETSTLFNVAIPTITSALCLKVLGWADRLAGKDAVDVWRLLRAHVQCIPGPIAW
                     RDSGAQGD"
BASE COUNT          276 a          503 c          504 g          258 t
ORIGIN      
        1 acgcgcactc ggccgtgttc ttcgttcaga caatcccgag gttagctcgg acccacgaca
       61 tccgagccgc gccacagcgc gacgcgctcc tcgtcgctca tgccatcacc agcgaggccc
      121 tcgaacgcgc ccgaacgaac gggtggtccg tggtgaccga cgacggtgcc ggctgggttc
      181 gccttgccga gcgaacgctg cacttcgaaa cgacgaccgc agcggacgcg cctaagcgac
      241 ctcctggtcg ccccggccgc ggcgtctttt ctgttgtgcg cacgctgttc acgatggacg
      301 aggatggtgc ccaccagcag gaaatcgccg agttcgcgca catccgccaa gccgcagttt
      361 ccaaggcact caacaggctt gcccaattag acctcgtcgc gcgtgggccg aacggctggc
      421 aggtaatcaa ccgcgccgga gctatctcct ggtggcttga gaactacccc ggaccgggtg
      481 gcatcgagac gcactggttc ggggtggact ccatcagaga gcaggcatat gacgcgtacg
      541 ctgcgctcga tcagatgcat gcgcgtccgc tcatctcggg cgatgtcgcg gccgacctcg
      601 tcgcgccatg gcggacgccg aggcgggcgg tgctgtatgc caagcgtggc gccgacctct
      661 ccagtgtcgg gctgacaccg agctacgccc cgacagcgac cttgaccctg gtgctgccac
      721 aagacctcgg agtgtggccg ctacccaagg cccccgccct catcgagatg caaggccacc
      781 atgtcgcacg ggcgaacgcc ttccaagtgc tgttcgacct tgcacgcagc ccgggtcctg
      841 acgtaagtga ggcgctggag gcgtggcgaa cgtggatggt ccaaccaggc tcgttgttgt
      901 gaccgagttg cgactgcatt ctacatcgcg cgccgaggac gccggcttcc ttgcgatgcg
      961 cgacatcgcc gcagtggccg acgagaccgc gatcgggtac cgaatcatcg gtggacaaat
     1021 ggttcggctt catgtggccc ttgccggcgt aacggagccg acagtgagag tgacccaaga
     1081 cgctgacatg ggaatcgagg cggcttcggc acgcaacccg gcgctggtcg ccgggctcga
     1141 agtgctcggc tatacgcgcc cggaggcgtc gaaccgattc atcagggcaa cagagagcgg
     1201 cctgagtctc gtgattgacg tgctcactcc ggcatggggc aggcggatgg tgtccaacca
     1261 gcggcacggc gagatgacag ttgacgagat ccccggtctg tcgctcgcgc tggccggaac
     1321 tggagagaac attgacctag ccgtgacgct gctcgatgag accagcacgc tgtttaacgt
     1381 cgccataccc acgatcacgt cagccctctg cctgaaggtg ttgggctggg cagaccgcct
     1441 ggccggcaag gacgcggttg acgtttggcg actgctccgc gcgcacgtcc agtgcatacc
     1501 tggaccgatc gcctggcggg actcaggcgc gcagggggac g
//
LOCUS       DASWVF010000035         7195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_128369, whole genome shotgun sequence.
ACCESSION   DASWVF010000035 DASWVF010000000
VERSION     DASWVF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128369"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="hrcA"
                     /locus_tag="VGD55_00585"
     CDS             <1..922
                     /gene="hrcA"
                     /locus_tag="VGD55_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0868870.1"
                     /translation="HRGVAPATGRNDMAALEEEGYIAQPHTSAGRVPTDKGYRLFVDR
                     LSTVKPLSGAERRAIEQFLDGSLDLDDMVARTVRLLAQLTRHVAVVQYPILDRSSVRH
                     LELIKLSDTRLMLVLITDTGRVEQRQVDLPGLIDEHTVGDLRTRLNVRIAGKAIRDVP
                     IELADLAEAEPIATRGAASAVVAVLLASLVERREERVVVGGASNLTRLALDFPDSVHP
                     VLEALEEQVVLIRLLGEVNRPTEVTVRIGDENSHEGLRSTSVVSATYGSGTRALASLG
                     VLGPMRMDYPGTMAAVGAVARYVGRILAES"
     gene            960..2084
                     /gene="dnaJ"
                     /locus_tag="VGD55_00590"
     CDS             960..2084
                     /gene="dnaJ"
                     /locus_tag="VGD55_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0868871.1"
                     /translation="MARDHYAILGVRKDAPPEEIKRAYRRLARQLHPDVNPDQQTQER
                     FKEITAAYEVLSDPSKRQLYDMGGDPLSASGNSGFGAGFGGLGDIMDAFFGGGAARGP
                     RTRTRKGNDALIHVEIDLIEAVFGATKELNIETAVVCATCNGAGTASGTHPDTCQVCK
                     GRGEISQVTRSFLGQVMTSRPCPQCGGVGTVIRHPCAECSGDGRVRARRTIEVAIPAG
                     VEHGMRLRLTGEGEVGPGGGPPGDLYVEIEERKHPTFERHGTDLHAALAVPMTAAALG
                     TSLKLETLDGPVDIDVKPGTQSGTVIPLRGRGVPRLRSNSRGDLHVHLEVSTPTKLDD
                     EQEQLLRKLASLRGEETPDGSFKATTSGLFGRIRDAFNGR"
     gene            2093..2851
                     /locus_tag="VGD55_00595"
     CDS             2093..2851
                     /locus_tag="VGD55_00595"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0868872.1"
                     /translation="MTAPLFFADADQLRAASIGDVVELGGAEGRHAATVRRLVAGEKL
                     DLADGAGLVCSAEVTAVLAHGLQARVLARTEVPRPPVTVTVVQALAKGDRDERAVETM
                     TEVGVDVIVPWQAGRSIVRWDSPRAQKAVTRWRTTAREAAKQARRAWLVDVPPLESTH
                     AVIARIDSVVRGGGVAVVLHEESDRPIAGLAVPKRGEVVLVVGPEGGITPDEIAAFEA
                     VGAAVHRLGPTVLRTSTAGTVAAAIVMASSGRWS"
     gene            complement(2848..3342)
                     /locus_tag="VGD55_00600"
     CDS             complement(2848..3342)
                     /locus_tag="VGD55_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0868873.1"
                     /translation="MPETQREKVPRNDGGELDTATAFLAFARHCLIKKTQGLDDEQLR
                     RVLVDSGTSLLGLIQHSVVGERWWFGYHLLGVGEEDDFDFTMTVPPERNAADIVQDYR
                     NAIDESDATIRRIGDPEALTARPIDDRPLSLRWVLAHMTSETARHAGHADIIREQIDD
                     VTGR"
     gene            complement(3510..4505)
                     /locus_tag="VGD55_00605"
     CDS             complement(3510..4505)
                     /locus_tag="VGD55_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868874.1"
                     /translation="MTALSVPAQPRRDAGARPVPWRRMGWVTWRQHRLTLAGLTAVLG
                     AVSVYLLVTGMHVRGTGTGGYSAMGPFTLTSALFQVIPPLIGAFVGAPVLARELESGT
                     FRFTWTQGFGRTRWTIATLTPLAVAVTLQAGAVGVLYRWCYGPMIGGKYGVSPLTGNT
                     FDIHGLAFAAWTLTAFSIGALAGGLIRRVVPAMFATLAAWSGLAVVTAVYLRPHYEAL
                     LVTNNPNLPTGAWVKSQWWAQSGNPISRATVNHVLQQGGITSVPDQFPRTPPGSTAAN
                     TDPMQYLLHHGITFVSSYQPASRFWPFQWIEGSWLLLLSLVLTGTVVWLVQRRAT"
     gene            complement(4502..5419)
                     /locus_tag="VGD55_00610"
     CDS             complement(4502..5419)
                     /locus_tag="VGD55_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0868875.1"
                     /translation="MNVIEASGLGKRYGSTWALHGCSLAIPDGHVVALVGPNGAGKTT
                     LLNLAAGLTGPTAGSITVLDGAPPGSPAALDGIAFVAQDIPLYKNLSVADMLHVTRNL
                     NWRFDHAYATARLHDLAIPPSRKAGKLSGGQQAQLALTLALARRPSLLVLDEPMAMLD
                     PLARHDFMALVMTAMADDGVSVVLSSHVLAELERVADYFVLLSGGSVQVAGEVDDLLA
                     SHRVLTGPAAEADRYVRQLRVVHARRGGAQAHLLTRTNGSLDPVPPGWEAHPVSLEEL
                     ALAYLREPGAASLPGPVPAQHTKPSEVAQ"
     gene            complement(5416..5940)
                     /locus_tag="VGD55_00615"
     CDS             complement(5416..5940)
                     /locus_tag="VGD55_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0868876.1"
                     /translation="MVVGQDDSDQQDSPSGADKRDAPPRHTSPLEFRLDLTSGVPTYL
                     QLVHQVEHALRLGYLAPGDQLPKVRDVVALLAINPNTVLKAYRELENKGLTAGRPGQG
                     TFIQATLSQVALPELAVLRRSLLGWLATAETAGLEQDGMVALFSSVLRDFHERDGTTV
                     DVRGLTRDEAEGVA"
     gene            6195..6434
                     /locus_tag="VGD55_00620"
     CDS             6195..6434
                     /locus_tag="VGD55_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868877.1"
                     /translation="MGDEPLPASLTLDPAFRAAYFLTKQYVALERQPDVGLGLYEQYL
                     HSDPARWHDGTQAVRRALSDDRPSDPLSENLDRGE"
     gene            6501..7109
                     /locus_tag="VGD55_00625"
     CDS             6501..7109
                     /locus_tag="VGD55_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY0868878.1"
                     /translation="MPSAELEELVVADSAALRTWLLAHHATSAGVWLALAKKGGARTT
                     LTWQQAVDEALCVGWIDGQARKRDDETSSIRFTPRGPRSSWSERNVGHVARLETEGRM
                     LAAGRAAVEAAKADGRWQAAYAPQSAAEMPTDLVEAIAANPDAQTMFDVLTKANRYAL
                     IYRLGSVKRQETRERKIGEFVAMLARHETIHPQTARPADPAS"
BASE COUNT         1199 a         2361 c         2467 g         1168 t
ORIGIN      
        1 ccaccgcggg gttgcgcccg cgaccgggcg caacgacatg gcggcgctcg aggaagaggg
       61 ctacatcgcc cagccccaca ccagcgccgg gcgggtgcca accgacaagg gctaccggct
      121 tttcgtcgac cggctgtcga ccgtcaaacc gttgagcgga gccgagcggc gggcgatcga
      181 gcagttcctc gatggttcgc tcgacctcga cgacatggtg gcgcgaaccg tgcggttgct
      241 cgcgcagctg acccgccacg ttgccgtcgt gcagtacccg atcctcgacc gctcgtcggt
      301 gcgccacctc gagttgatca agctcagtga cacccgattg atgctggtgc tgatcaccga
      361 cacgggccgg gtcgagcagc ggcaggtcga cctgcctggc ctgatcgacg agcacacggt
      421 cggcgacctg cggaccaggc tgaacgtccg catcgccggc aaggcgatcc gcgacgtgcc
      481 gatcgaactg gccgacctcg ccgaggccga acccatcgcc acgcgcggcg cggcgtcggc
      541 ggtcgtcgcc gtgttgctcg cttcgctggt cgagcgccgc gaggagcgcg tcgttgtcgg
      601 cggggcaagc aacctgacgc ggctggcatt ggatttcccc gactcggtgc atccggtgct
      661 tgaagcgctc gaggaacagg tcgtgctgat ccgcttgctc ggggaggtca atcgcccgac
      721 cgaggtgacc gtgcggatcg gcgacgagaa ctcgcacgaa ggtcttcggt cgacgtcggt
      781 cgtgagcgcc acatacgggt cgggaactcg ggcgctggcc agcctcggcg tgctcgggcc
      841 gatgcgcatg gactatcccg gcacaatggc cgcggtcggc gctgttgcgc gctacgtcgg
      901 ccgcatcctc gccgagtcct gaacccgagt cctgaacaag cagaagctag gagttttcgt
      961 tggcccgcga ccactacgcg atcctcggcg tgcgcaaaga cgcaccgccc gaggagatca
     1021 agcgtgcgta ccggcgccta gccaggcagt tgcacccgga cgtgaatccc gaccagcaga
     1081 cgcaggagcg gttcaaggag atcaccgcgg cctacgaggt gttgtcggat ccgagcaagc
     1141 gacagctgta cgacatgggc ggcgaccccc tgtcggcgtc cggcaatagc ggttttgggg
     1201 ccggattcgg tgggctcggc gacatcatgg acgcgttctt cgggggcggt gccgcgagag
     1261 ggccgcgcac gcgcacccgc aagggaaatg acgcgctcat ccatgtcgag atcgacctca
     1321 tcgaggcggt ctttggtgcc acgaaagagc tcaacatcga gacggcggtt gtctgcgcga
     1381 cgtgcaacgg tgcgggcact gcatcgggca cgcatcccga cacctgccaa gtctgcaagg
     1441 ggcgcggcga gatcagccag gtgacccgat cgttcttggg tcaggtcatg acgtcgcggc
     1501 cgtgcccgca atgcggcggc gtcggcaccg tgatccggca cccgtgcgcc gagtgttccg
     1561 gcgacggccg ggtgcgcgcg cggcgcacga ttgaggtcgc gattccggct ggggtcgagc
     1621 acggcatgcg gctgcggttg accggtgagg gcgaggtcgg cccgggcggc ggaccgcccg
     1681 gcgacctcta cgtcgagatc gaggagcgca agcacccgac gttcgagcgg cacggcaccg
     1741 atctgcacgc cgcgctcgcc gtgccgatga ccgctgccgc gctcggaacc tcgctcaagc
     1801 tcgagacgct cgacggcccc gtcgacatcg acgtgaaacc cggcacgcag tcgggcacgg
     1861 tgatcccgtt gcgtggtcgc ggggtgccgc ggttgcggtc gaacagccgc ggcgacctgc
     1921 acgtgcacct cgaagtctcg accccgacca agctcgacga cgagcaggaa cagttgctcc
     1981 gcaaactcgc gagcctgcgc ggggaagaga cgcccgacgg ctcgttcaag gccaccactt
     2041 ccggactctt cggccgcatc cgcgacgcct tcaacggccg ctgaacctca tcgtgacagc
     2101 accgcttttc ttcgcggacg ccgaccagct gcgtgccgcg tcgatcggtg acgtcgtcga
     2161 acttggtggc gctgagggtc ggcacgcggc caccgtccgg cgcctagttg caggcgaaaa
     2221 gctcgatctg gcggacggcg cggggctggt gtgttcggcc gaagtgaccg cggttttggc
     2281 tcacggactg caggcccggg tgctcgcccg caccgaagtt ccgcggccgc cggtaacggt
     2341 gaccgtggtg caggctctcg cgaagggcga ccgtgacgag cgggcggtcg aaacgatgac
     2401 cgaggtcggc gtcgacgtca tcgtgccgtg gcaggccggg cgctcgatcg tgcgctggga
     2461 ttcgccgcgc gcgcagaagg cggtcacccg gtggcgcact accgcgcgtg aagcagccaa
     2521 acaggcgcga cgggcttggc tggtcgacgt cccgcctctt gaatcgacgc acgccgtcat
     2581 cgcgcggatc gattccgtcg tgcgaggcgg aggtgtggcc gtcgttctgc atgaagaatc
     2641 cgaccggccg atcgcgggtc ttgcagttcc gaagcgcggc gaggtcgtgc ttgtggtcgg
     2701 ccccgaaggc ggcatcaccc ccgacgagat tgccgcgttc gaagcggtcg gggccgccgt
     2761 ccaccggctc ggcccgaccg tgctgcgcac ctccacagcc ggcaccgtcg cagccgcaat
     2821 cgtgatggca agctctggcc gctggagcta gcgacctgtc acgtcgtcga tctgctccct
     2881 gatgatgtcg gcgtggccgg cgtgccgtgc cgtctcgctc gtcatgtgcg ccagtaccca
     2941 tctgaggctg agcggtctgt cgtcgatcgg ccgtgcggtg agggcctcgg gatcaccgat
     3001 gcgtctgatc gtggcgtcgc tctcgtcaat cgcgttgcgg tagtcctgca cgatgtcggc
     3061 ggcgttgcgc tccggcggca ccgtcatggt gaagtcgaag tcgtcctcct caccgacgcc
     3121 gagaaggtgg taaccgaacc accaccgctc gccaacgact gagtgctgaa tcaagccgag
     3181 cagcgacgtg ccgctgtcga ccagtactcg tcgcagttgc tcgtcgtcca gcccctgcgt
     3241 cttcttgatc aggcaatggc gagcgaacgc caggaaagcg gtcgcggtgt cgagttcgcc
     3301 gccgtcgttg cgcggcacct tctcgcgttg tgtctcgggc atgcctaaaa cctacgtcgg
     3361 ggcgtgggat tactacggca acaggcgggg tttttggcgc atacgtttgc tagcgaacgc
     3421 acacttctgc cgatgtgccg atgtcgggtc aatgcaccct ccatgctggt cagcgcctga
     3481 acatacggtt gcgacccggc gccgcctcct catgtagcgc gtcgttgcac gagccagacg
     3541 acggtgccgg taaggacgag cgacagcagg agcagccagc tcccctcgat ccattgaaat
     3601 ggccagaacc tgctggccgg ctggtagctc gacacgaaag taatgccgtg gtggagcaga
     3661 tattgcatag ggtccgtgtt tgctgcggtc gagccgggcg gcgtccgggg aaattggtcg
     3721 gggaccgaag ttatgccgcc ctgctgcagt acgtggttga cggtggcgcg actgatcggg
     3781 tttccgcttt gagcccacca ctgactcttg acccacgcgc cagtaggcag gttgggattg
     3841 ttggtgacca atagcgcctc gtagtgcggg cgtaggtaga ccgccgtcac aacggcaaga
     3901 ccgctccacg cggccaaggt ggcgaacatg gcggggacaa cccggcggat caggccgccc
     3961 gcgagtgcgc ctattgagaa cgccgtcagc gtccacgcgg cgaacgccag cccatggatg
     4021 tcgaacgtgt tgccagtgag cgggctcacc ccatacttcc cgccgatcat cggcccgtag
     4081 caccagcggt agaggacgcc gaccgccccg gcttggagcg tgaccgcgac ggcgagcggc
     4141 gtcagtgtgg cgatggtcca ccgcgtccgc ccgaagccct gggtccaggt gaaccggaag
     4201 gtgccgctct ctagctccct ggccagcacc ggcgcgccga cgaacgcgcc gatcagtggc
     4261 ggaatcacct ggaacagggc cgaggtcagc gtaaaaggcc ccatagcaga gtagccgccg
     4321 gtcccggtgc cgcggacgtg catgccagta acaagcaggt acacactcac ggcaccgagc
     4381 acggcggtga gaccggccaa cgtcagcctg tgctgtcgcc aggtgaccca acccattctc
     4441 cgccacggca cgggccgggc gccggcatcg cgtcgcggct gcgcgggcac gctcaaagca
     4501 gtcactgcgc cacctcgctc ggtttcgtat gttgggcggg taccggtccg ggcagcgaag
     4561 cagcgccggg ctcccgcaga taggccaggg cgagttcttc gaggctgaca ggatgcgcct
     4621 cccagcccgg cggcaccggg tcgaggcttc cgttcgtccg ggtcagcagg tgggcctgcg
     4681 ccccgccgcg tcgggcgtgc accacgcgca gctgtcggac gtaccggtcg gcttcggcgg
     4741 cgggtcccgt cagcacacga tgggaagcga gcaggtcatc gacctcgccg gccacctgga
     4801 cgctgccgcc cgacagcagg acgaaatagt ccgccacccg ctcgagctca gctaggacgt
     4861 gggaggacaa caccaccgac acgccgtcgt cggccatggc agtcatcacc aaggccatga
     4921 agtcgtggcg ggcaagcgga tcaagcatcg ccatcggctc gtccagcacc aggagcgatg
     4981 gccgccgcgc caacgccagc gtcagcgcga gttgcgcttg ctgacctcca gacagtttgc
     5041 cggccttgcg actgggcggg atagcgaggt catgcaagcg ggctgtggca taggcgtgat
     5101 cgaagcgcca gttcaggttc cgggtcacgt gcaacatgtc ggcgacagac aggttcttgt
     5161 acagcgggat gtcttgggcg acgaaggcga tgccgtcgag cgcggccggc gacccaggcg
     5221 gcgcgccgtc gaggacggtg atgctgccgg cggttggtcc cgtcaggcca gccgccaggt
     5281 tcagaagggt ggtcttgcct gcgccgttgg gtccgaccag cgcgaccacg tgcccatcgg
     5341 ggatggcgag cgagcacccg tgcagcgccc acgtgctgcc ataacgttta ccgaggccgc
     5401 tggcttctat gacgttcatg ccacgccctc cgcctcgtcc cttgtcaggc cacggacatc
     5461 gaccgttgtc ccgtcacgct catggaaatc ccgcaacacg ctgctgaaca gcgccaccat
     5521 cccgtcctgc tctagccctg cagtctcggc agtcgccagc cagccgagca gcgaacgccg
     5581 aagcaccgcc agctccggga gcgccacttg gctgagggtg gcctggatga acgtgccctg
     5641 gccgggccgc ccagccgtaa ggcccttgtt ttccaactcg cggtacgcct tgagcacggt
     5701 gttcgggttg atcgccagca aagccaccac atccctgact ttgggcagct gatcgccggg
     5761 tgcgaggtag ccgagccgca gcgcgtgctc gacctgatgg acaagctgca ggtacgtcgg
     5821 tacgccagag gtgaggtcga ggcggaactc taggggggat gtgtgccggg gcggggcgtc
     5881 acgcttgtct gcgcctgacg gcgagtcctg ctggtcgctg tcatcttgtc ctactaccat
     5941 aagacaaagg tacagggacc gaattggctc gtcaatgccc actcccaccg cgaagagacc
     6001 accgagcatc tgggcgcgcc aacacatcaa tctgccgccg tccgtgtacg tcggcgccgg
     6061 tcagcgtccg ctcgggaact tgatggcgga cgtcctacgc gaggcagcgt tgtattcggt
     6121 aatgccgcga tgacgcatgt gcgatcgtgc cgagctgagt gcgtcgtgat cctcgtcggg
     6181 tgggggctta ccgaatgggt gacgaaccac taccggccag cctcacgctg gacccggcct
     6241 tccgtgctgc ctatttcttg acgaagcaat acgtggcgct cgaacggcag ccagacgtcg
     6301 gcctcgggct atatgagcag tacctccact cagatccggc acgctggcac gacgggacgc
     6361 aggccgttcg gcgggcgctc tccgacgatc ggccgtccga tccgctctcc gaaaacctcg
     6421 accggggcga gtagacccga acgcacggat ttgccccgac ccgcgttcag ctgtcggtgg
     6481 gttcccttac cctgtcgccc atgccgagcg cagagctgga ggaactggtc gttgctgatt
     6541 cagcagcgct ccgcacttgg ttgttggccc accacgccac gtcagcgggg gtgtggctgg
     6601 ctcttgccaa gaagggcggc gcacgcacga cgttgacctg gcagcaggcg gtcgacgagg
     6661 cactgtgcgt cggttggatt gacgggcagg cgcgcaagcg tgacgacgaa acgtcctcga
     6721 tccggttcac accgcgtggg cctcgcagtt cgtggtctga gcggaacgtc ggtcatgtgg
     6781 cgcggctgga gacggagggc cggatgctgg ccgccggccg agcggcggtt gaggcggcga
     6841 aggccgacgg gcggtggcag gctgcgtacg caccgcagtc agcggcggag atgccgaccg
     6901 acctggtgga ggcgatcgcc gctaacccgg acgctcagac aatgtttgac gtgctgacga
     6961 aagccaaccg ttatgccctc atctatcgac ttggctcggt gaagcggcaa gagacgcgcg
     7021 agcgcaagat cggcgagttc gtcgccatgc tcgcccgcca cgagacgatt catccgcaga
     7081 ccgcgaggcc agcagacccg gctagttgac agtgaacatc ttgtcgtcga cgtaggtgac
     7141 cgagccgtcc ttcgccgaga cctcatacgc cctgacgacg tacgggcccg gcgcg
//
LOCUS       DASWVF010000036         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_357722, whole genome shotgun sequence.
ACCESSION   DASWVF010000036 DASWVF010000000
VERSION     DASWVF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357722"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1604)
                     /locus_tag="VGD55_00630"
     CDS             complement(165..1604)
                     /locus_tag="VGD55_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868879.1"
                     /translation="MTQQPPDDFERRLSDVLHSRNLGVPVPPDAIDRIHAGARRRQQR
                     RTGASALGAVAVIAIAAVTIGVRPHGHASNVAGNLQTASPSLTAAPASSAAPSARRFG
                     VASPQVSASATPASSAPAINTLAQPLASETSAVGQPVGFTPVSVSAVGLNDYWVLGYT
                     TKKTSDGIDINTTVEQTTDGGQHFAIDTTPPAIVGQAPIKGPAGATTISQIRFGDAKD
                     GWVFGSALFSTTDAGSSWTAVNGIAGGVVDLVAANNTAWAIVQTASSSGASPSATSDQ
                     YALYSTSYGKGVQSWAPVALPISLGSTTPSIVDQDGTVTVMASGPSRAGNKVHVLVGV
                     AGKPFTDHTGPCEQDLGGTLSNSKTAIWAECPGGTSASLYVSKNAGATWTASAVNDPR
                     KIELQRGEAIGAIDDTTAVIFDATAQGLGKISGATVTPTSATNVSDVTFLGFTTTAVG
                     FAITVGEDGTTTQLLRTMDGGATWPAVTF"
     gene            complement(1601..>1953)
                     /locus_tag="VGD55_00635"
     CDS             complement(1601..>1953)
                     /locus_tag="VGD55_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0868880.1"
                     /translation="YVRRIMVNRNISIWRRHRRETLVLDVPEDAGRRGAGPSEPVVRD
                     LALWERIRALPPRQRTVIVLRYYEDMSEAQIASVMGSSVGTVKSQASRALASLRRDAY
                     QFAESDGPAEGVGA"
BASE COUNT          324 a          645 c          630 g          354 t
ORIGIN      
        1 ctgcgccaag tgcgcttgca ccaactcgtc gtcgctggcg gcgccgtgcg ccggcgtgga
       61 tgcgatccat tgcgtccgcc gagacgggca caccgagacc gcgagaatgc aggacggcgc
      121 gtagccggcg ttcgagctta tcgtcgccgt tggtgctcat cgacttagaa cgtcacggcg
      181 ggccacgtcg cgccgccgtc cattgtccgc aggagttggg tggtggtgcc gtcctcgccc
      241 accgtgatgg cgaatccgac cgcggtcgtc gtaaagccca agaacgtgac gtccgagaca
      301 ttggtcgcgc tcgtcggggt gacagtcgcg ccagaaatct tgcctaggcc ctgagcagtg
      361 gcatcgaaga tgaccgcggt ggtgtcgtcg atcgcaccaa tcgcctcacc cctctgcaat
      421 tcgatcttcc ggggatcgtt gactgcgctc gcagtccatg tcgcgcccgc gttcttggag
      481 acgtacaaac tcgcgctggt accgccgggg cactctgccc agatcgcagt cttcgaatta
      541 gacagcgtgc cgccgaggtc ttgctcgcac ggcccagtgt gatcggtgaa cggtttaccg
      601 gccacgccga cgagcacgtg caccttgtta cccgcgcgcg acggacctga cgccatcacc
      661 gtgacggtgc cgtcctggtc gacgatgctc ggcgtcgtcg aaccgaggct gatcggcagc
      721 gcgaccgggg cccacgattg cacgcccttg ccgtagctcg ttgagtacag cgcgtactgg
      781 tcgctcgtcg ccgaagggct tgcgccgctg ctcgacgcag tctgcacgat cgcccaggcg
      841 gtgttgtttg cggccacgag gtcgacgacc ccaccggcaa tgccattcac ggcggtccac
      901 gacgaccccg catccgtcgt tgagaacaag gccgaaccga acacccagcc gtccttcgcg
      961 tcaccgaagc ggatctgcga gatcgtcgtg gcaccggcgg gccccttgat cggggcttgg
     1021 ccgacgatgg ccgggggagt tgtgtcgata gcgaagtgct ggccaccgtc cgtcgtttgc
     1081 tcgaccgtcg tgttgatgtc gatgccgtca gacgtttttt tggtcgtata cccgagcacc
     1141 caatagtcat tgaggccgac ggcactgacg gagacaggcg taaatccaac gggctggccg
     1201 acggctgagg tctcagatgc caaaggctgg gcgagcgtat tgatggccgg cgcgctactc
     1261 gctggtgtgg cggacgccga aacctgcggc gatgcgaccc caaagcgcct cgcactaggc
     1321 gccgcactac tcgctggtgc ggctgtcaac gacgggcttg cggtttgcag attgccagcc
     1381 acgttcgatg cgtgaccatg cgggcgcacc ccgatagtga ccgcagcaat cgcgatgacc
     1441 gcgaccgcac cgagcgcgct tgcgccagta cgtcgctgct ggcgtcggcg cgcgccggcg
     1501 tggatgcgat caatcgcatc aggcggaact ggcacaccaa gatttcgaga atgcaggacg
     1561 tcgcttaggc ggcgctcgaa gtcgtccggc ggttgttgcg tcatgcgccg accccttcgg
     1621 ctggtccgtc agattcggcg aactggtacg cgtcgcggcg cagtgacgcg agtgcgcgcg
     1681 atgcctgact cttgaccgtg cccaccgacg accccatcac cgaggcgatc tgcgcctctg
     1741 acatgtcctc gtagtagcgc aacacgatga ccgttcgttg ccgcggcggg agtgccctga
     1801 tccgctccca caacgcgaga tcgcgcacga ccggttcgga ggggcccgcc cctcgccgtc
     1861 cggcatcttc aggcacgtct aacaccagcg tctcgcgacg atgacgtcgc cagatgctga
     1921 tgttgcggtt caccatgatg cgccgcacat acc
//
LOCUS       DASWVF010000037         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_459665, whole genome shotgun sequence.
ACCESSION   DASWVF010000037 DASWVF010000000
VERSION     DASWVF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459665"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGD55_00640"
     CDS             complement(<1..343)
                     /locus_tag="VGD55_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868881.1"
                     /translation="MTALDTAASSAADDIVFDVDRVTLRFGGVTSLNAVSLQMHRGEI
                     LAVIGPNGAGKTSLFNSLTGVYRPQEGSILVSGRAGDPLVSVLGMPTHKVNHLGVART
                     FQNIRLFPALTA"
     gene            complement(351..>1677)
                     /locus_tag="VGD55_00645"
     CDS             complement(351..>1677)
                     /locus_tag="VGD55_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0868882.1"
                     /translation="VVGSIITVVTLFDISKLGAHDKVDSATGPWQNLGSGGWMACAGL
                     FLLGAAGYVAATAPREGDAVDAYATSDVVGGTAKAGARRAVGYLSAPGAARSALLIAV
                     AVALFYPPTATPFWQEVVVSEIGISMLLAVGLNVVVGWAGLLDLGFIAFYAIGSYTTA
                     YLVGSLPVKPPHWLHMTPLLTIPFAIVVCLLAGLALGAPTLRLRGDYLAIVTLGFGEI
                     IHLAAVNSRGITNGSEGLTNDTARHVKSVPNPSIHIGPIHARWGLNQLQYWYLLLIFL
                     VVIILAFRRLEQSRLGRAWAAVREDEVAAQATGINTVKVKLAAFAIGASTSGVAGVFF
                     ASQIGSITPDNFVLNNSILIVAYVVFGGMGSLQGALAGAAMLTWLPEFLKYQVPPSDK
                     LMWIGAVVLLMMIFRPAGLIPARRRKAELSGLDAPSSAETAAVAASEGL"
BASE COUNT          345 a          538 c          523 g          271 t
ORIGIN      
        1 acgcagtcaa cgccgggaag aggcggatgt tctggaacgt ccgcgccacg ccgaggtgat
       61 tcactttgtg ggttggcatc ccgagcacgc tgaccagcgg gtccccagcg cggccagaga
      121 cgagaatcga gccttcctgc ggccggtaaa cgcccgtcaa tgaattgaag agtgatgtct
      181 tgccggcgcc attcggcccg atgaccgcga ggatctcgcc tctgtgcatc tggagcgata
      241 ccgcgttgag gctggtcact ccaccgaagc gcaacgtgac gcggtcgacg tcgaagacga
      301 tgtcgtctgc tgccgaactg gccgcggtgt cgagcgccgt catcgtggca tcacagccct
      361 tccgaggcgg ctactgcggc cgtctcggcg ctcgatggag cgtcgaggcc agacagttcg
      421 gccttgcgcc gtctcgcggg tatgaggccg gcagggcgga agatcatcat caacaatacg
      481 acggcgccga tccacatcag cttgtccgac ggcggaacct ggtacttcaa gaactccgga
      541 agccatgtca gcatcgccgc gcctgctagc gccccctgca acgaccccat gccgccgaag
      601 acgacgtacg ccacgatcaa aatcgagttg ttcaacacga agttgtcagg tgttatcgac
      661 ccgatctgac tcgcgaagaa cacaccggcg acaccagacg tcgacgcccc gatcgcaaac
      721 gcggccagct ttaccttgac cgtattgatg ccggtcgctt gcgctgcgac ctcgtcctcg
      781 cgcacggctg cccacgcgcg accgagcctc gattgctcga gcctgcggaa cgccaagatg
      841 atgacgacaa ggaagatcag cagtaagtac cagtactgca gctggttgag cccccatctc
      901 gcatgaatgg gtccgatgtg aatcgacggg ttgggcacag acttcacgtg gcgcgcggtg
      961 tcattcgtga gtccttcact gccgttggtg atcccacgcg agttgaccgc agcgaggtga
     1021 atgatctccc cgaagccgag ggtcacgatc gcgaggtagt cgcctcgtag tcgcagcgtg
     1081 ggcgcgccta aagccaagcc ggcaagcaaa cagaccacga tcgcgaacgg gatcgtcagc
     1141 agcggcgtca tgtgcagcca gtgcggaggc ttcacgggca gcgagcccac gaggtacgcc
     1201 gtcgtgtatg agccgattgc atagaaagcg atgaatccga ggtcgagcag accggcccaa
     1261 ccgacaacca cgttgagtcc gaccgcgagc agcatcgaaa tgccgatctc cgacacgacg
     1321 acctcttgcc agaatggtgt cgcggttggc gggtagaaca acgcaactgc taccgcgatg
     1381 agaagtgccg agcgagccgc acctggcgca ctgagatagc ccacagcgcg acgcgctccc
     1441 gctttggcag tgccaccaac cacatcggac gtggcgtagg cgtcgaccgc atcaccttcg
     1501 cgcggcgcgg tcgcggcgac gtagcccgcg gcgccgagga ggaacaggcc agcgcacgcc
     1561 atccagccgc cggagccgag gttctgccaa ggcccggtgg cgctatcgac tttgtcgtgc
     1621 gcgcccaact tgctgatgtc gaacagcgtc accaccgtga tgatcgagcc gacaacg
//
LOCUS       DASWVF010000038         5264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_306762, whole genome shotgun sequence.
ACCESSION   DASWVF010000038 DASWVF010000000
VERSION     DASWVF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306762"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..689)
                     /locus_tag="VGD55_00650"
     CDS             complement(195..689)
                     /locus_tag="VGD55_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868883.1"
                     /translation="MRVPAGLVGGAVGLAVGLAVGLADGLGVGPVLGFGVTVAVGFGD
                     PDGFGDPDGDGLLVVDAEADAFAEPDALTEADADVDPDGDVEPLGLVLVVADGDGVGV
                     GDHEGDGGGGGGGLRATVAAPSTVPSRSNTGLGAGTMPSAIARAPNACGACASATAWL
                     TACT"
     gene            1035..2777
                     /locus_tag="VGD55_00655"
     CDS             1035..2777
                     /locus_tag="VGD55_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0868884.1"
                     /translation="MRKIARLGMPHRRLHAGLLVIGFVMIIFTGRLLQLQGVDSTVYA
                     ADAHAQYLHKVTLPAQRGEILDRNGAVLADTVNAFDIAGDPLVATGSTTTSPWAMAGK
                     LAPLLGVNQLTLVSDLTGKNQFVYLAHQVTPQVEKQVLALKLPGISAESTNKRVYPAG
                     DVAANVLGFVGSDGKGLGGLEYQYQSTLSGKDGHLTVELGHNGQVIPDGVGSGDAAIP
                     GQGIELTLDRDIQWKAQQAIAAQVKVTGAAGGSVIVMQPRTGAILAMATVPTFDPANP
                     GDAPAQDRGNAALSDVFEPGSTNKVITMSAAIDSGVLTPMSPIDVPPTLVRANKVFHD
                     AESHGLEHLTLAGVLAESSNIGTILASEKVGVTRLYSYLEAFGLGQPSGLDFPGESQG
                     ILDPLKSWSPSQQYTVPFGQGLSVNALQVATVYSTIADGGVRVEPSLVKGFISSDGSI
                     KPATAPTQTRVVSESTATQVESMLEAVTTDQGTAPEARIPGYRVAGKTGTAQRVDPTC
                     GCYRGYTASFVGFAPADNPQLVVLVVLDNPINGHYGGSIAAPVFKDVMSFALESEKIP
                     PTGTTPPVIKLSLP"
     gene            2796..4355
                     /locus_tag="VGD55_00660"
     CDS             2796..4355
                     /locus_tag="VGD55_00660"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY0868885.1"
                     /translation="MPGQDARDGSSLSTSLRPQESFGRRLAGLADLLGVAAPEHDASI
                     SGVTHDSHAIRAGDLYAALPGSRTHGARFAADAVAAGAVGVLTDQTGKLLAGRLDVPL
                     LVVETPRARLGAVSSWIYGEPSTRMRVLGVTGTNGKTTTSYLLDAGLRAAGETTGLVG
                     TIETRIGDIGLASARTTPEAPDLQALLALMVEHGVGSVAMEVSSHALALNRVDGTSFE
                     VVAFSNLSQDHLDFHLNLDDYFEAKARLFTPEFAPLGVVNIDDEHGRQLRRSAPLATT
                     TVSTLTPDADWSVSDVATDGTGSRFVVHGPSVEMATGIRLPGAFNISNALLAFATLVS
                     AGVDPRDAAAGIASVSVVPGRMEAVSAGQPFVALVDYAHTPDAVVTLLTTVRATTKGR
                     VIVVLGCGGDRDRAKRPLMGAAAARLADVAILTSDNPRSEEPEQIVVALLEGAMGVAA
                     AQRGEVIVELDRRAAIAIAVERARPGDVVVVAGKGHEQGQEVGGVVHPFDDRAVLRAE
                     LAAHAHAAGTS"
     gene            4352..>5264
                     /locus_tag="VGD55_00665"
     CDS             4352..>5264
                     /locus_tag="VGD55_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0868886.1"
                     /translation="MIALTIAEIAQLSNGTVSPFAEPTTLVAGPVVADSRLVVPGALF
                     VAIPGERVDGHQFAAQAMADGARAVLASRPVNVPAVVVDDTVAGLGVLARGYLNRLAA
                     HVGPTVIGITGSSGKTSTKDIVAQLLSNHGPTVAPAGSFNTEVGLPLTVLRADAATRY
                     LVLELSARGLGHIAYLCGIARPSIGVVLNVGAAHLGEFGSRDAVAQAKGELVEALPAD
                     GVAILNADDAAVAAMRLRTDARVVTFGVEAAADVSAVDIRLDELARPAFRIVTAAGEV
                     EVSLALHGAHHVLNALAAAAVALEVGVD"
BASE COUNT          855 a         1792 c         1749 g          868 t
ORIGIN      
        1 gcgccgcggc ggaacccggc cgcaccactg cgcgtcgtgc agtcgcgacc gtcggcggcg
       61 cgcactccgt ttgtgctgct cgtgtcgggc ctgctggcgg cggggctcgt cggcgttttg
      121 gttgtgaaca ctcgtcttgc acaaggctcg ttcgcagtga accaactcca gatcgagcag
      181 tcgaagtacg ccgatcaagt gcaggccgtc agccaagcgg tcgccgatgc gcaagcacca
      241 caagcattgg gcgcccgggc gatcgcgctc ggcatggtcc ccgcgcccaa tccggtgttc
      301 gagcgcgacg gcaccgtgct tggggcggcg acggttgctc gcaagccgcc cccacccccg
      361 ccgccatcgc cttcgtggtc gcccactcca acgccatcgc catcagcgac gaccagcacg
      421 agtccgagtg gttcaacgtc gccgtcggga tcgacatctg cgtcggcttc ggtgagtgcg
      481 tcgggttcag cgaaggcgtc cgcctcggcg tccactacta acaagccgtc accgtcggga
      541 tcgccgaagc catcgggatc gccgaagccg actgcgaccg tgacgccgaa gcccagcacc
      601 ggtccgacgc ccaagccgtc agccaaaccg acggccaaac cgacggccaa acccacagcg
      661 ccacccacca ggcccgcggg gacgcgcacg tgagtcggct ggatcctccg aacaacccga
      721 cgggacgccg acctgccccg cgcgctgcga cacctcacgt cgcgccatcg agggggagcg
      781 ctccacgcgg cagtgcgcca cgcggcagtg cgccacgcgg cagtgcgcca caatcccggt
      841 cggccggggc tccgccgcga cgcgaatcct tggggcgcga gccagtaggt cgccaagcgc
      901 tgcggggcga gcccatccgc cggcaaccgg cgcggccgca gcccccgcgc cagcaacctg
      961 gcccgcgcca aaccaacccg cgtcaactca ccgagcgtca tcccgtccgg cgtccaccca
     1021 accgcccccc acgcatgcgc aagatcgcgc gcctcgggat gccgcatcgc cgtttgcacg
     1081 cgggtctgct cgtcatcggc ttcgtgatga tcattttcac cggccgcctt ctccagcttc
     1141 agggcgtcga ctcgaccgtc tacgcggccg acgcgcatgc gcagtacctg cataaggtga
     1201 cgttgccggc gcagcgcggc gagatcctcg atcgcaacgg ggcggtgctc gccgacacgg
     1261 tcaacgcgtt cgacatcgcc ggtgacccgc tagtggcaac gggttcgacg accacgagtc
     1321 cgtgggcgat ggcggggaag ttggcgccgc tgctgggcgt caatcagttg accctcgtca
     1381 gcgatctcac cggcaagaac cagttcgtct acctggccca ccaagtgacc ccgcaggtcg
     1441 agaagcaagt cttggcgttg aagttgccag gcatctccgc agagagcacc aataagaggg
     1501 tgtatcccgc aggggacgtc gccgcgaacg tgctcggctt tgtggggtct gacggcaagg
     1561 gtcttggcgg gctcgaatat cagtaccaga gcacactttc gggcaaggac ggtcacctga
     1621 ctgtcgagct tggccacaac gggcaagtga ttcctgacgg cgtcggcagt ggcgacgcgg
     1681 cgatcccagg tcagggcatc gagctcaccc tcgaccgtga cattcagtgg aaagcgcagc
     1741 aggcgatcgc cgcgcaggtg aaagtcacgg gtgccgccgg cgggtccgtg atcgtcatgc
     1801 agccacgcac cggagcaatc ctcgccatgg cgacggttcc gacgttcgat cccgcgaacc
     1861 cgggcgatgc tcctgcacag gaccgcggaa atgctgccct gtcagatgtt ttcgagcccg
     1921 gcagcaccaa caaggtcatc acgatgtcgg cggcgatcga cagcggtgtg ctcacgccga
     1981 tgtcgccgat cgatgtgcca ccgacgctcg tccgcgcgaa caaggtgttt catgacgcgg
     2041 agtcgcacgg gctggagcac ctcacacttg ccggcgtctt ggcggagtcg agcaacatcg
     2101 gcacgatcct ggcgtcggag aaggtcggtg tgacgcggct ctactcctac ctcgaggcat
     2161 tcggcctcgg tcagccgtca ggtctggact ttcccggtga gagtcaggga atcctcgacc
     2221 cgctgaagag ctggtcacca tcgcagcagt acaccgtccc gttcggacag ggtctttcgg
     2281 tgaacgcact tcaggtcgcc acggtgtact cgacgatcgc cgacggcggg gtgcgggtcg
     2341 agccgagctt ggtgaagggc ttcatttcgt cggatgggtc gatcaagccg gcaacagcac
     2401 cgacgcagac gcgcgtcgtc agtgagtcga cggcgaccca ggtcgagagc atgctcgagg
     2461 cggtcaccac cgaccagggc accgcgcccg aggcacgcat ccccggctac cgggtcgcag
     2521 ggaagaccgg aacggctcaa cgcgtcgacc cgacgtgtgg ctgctatcgc ggctacacgg
     2581 cctcgttcgt gggcttcgct cccgccgaca acccgcagct ggttgtgctc gtggtgctcg
     2641 acaaccccat caacggtcac tacggcggat ccatcgccgc gcccgtcttc aaagacgtga
     2701 tgtccttcgc gctcgagtcg gagaagattc cgccgacagg cacgacgccg ccggtcatca
     2761 agctcagcct tccctgacgg gtagggtgga gcgccgtgcc cggccaagac gcgcgtgacg
     2821 gttcgtcgct gtcgacatcc ttgcgtccac aagagtcatt cggacgccga ttggccggcc
     2881 tcgccgacct cctcggcgtc gcggcacccg agcatgacgc gtccatctcc ggcgtcactc
     2941 atgactcgca cgcgatccgc gcgggtgacc tgtacgccgc attgccgggt agccggacgc
     3001 atggcgcgcg attcgccgcc gacgcggtcg ctgcgggtgc cgtcggcgtc ctgaccgacc
     3061 agacaggcaa gttgttggcg ggccggctcg acgtaccgct gctcgtggtc gagactccgc
     3121 gcgcacgcct cggcgccgtc tcttcgtgga tctacgggga gccgtcgacc cgcatgcgcg
     3181 tgctcggcgt caccggcacg aacggcaaga cgacgacgtc ctacctgctc gacgcaggtc
     3241 tgcgggcggc gggggagacg acggggctcg tcgggacgat cgaaacgcgc atcggcgaca
     3301 tcggtctggc aagtgcgcgc acgacaccgg aagcccctga tctccaggcg ctgttagcac
     3361 tcatggtcga gcatggcgtc ggctcggtgg caatggaggt ctccagccac gctctcgcat
     3421 tgaaccgggt cgatgggacg tcgttcgagg tcgtcgcctt tagcaacctg tcgcaggacc
     3481 atctggattt ccatctcaac ctcgacgact acttcgaagc gaaggcgcgg ctcttcactc
     3541 cggagttcgc acctcttggc gtggtcaaca tcgacgacga gcacggccgc caacttcgtc
     3601 gaagcgctcc gctcgccacc accacggtct cgacactcac gccggacgcc gattggtcgg
     3661 tcagcgatgt ggcaaccgat ggcaccggct cgcggttcgt cgtgcacggt ccaagtgtcg
     3721 agatggccac cggcattagg ttgccgggcg cgttcaacat ctccaatgcc ctgctcgcgt
     3781 tcgcgaccct cgtgtcggcc ggcgtcgatc cccgcgacgc ggccgcgggg atcgccagtg
     3841 tctctgtcgt gccgggccgc atggaggcag tctcggccgg ccagccgttt gtcgcactgg
     3901 tcgactacgc ccacaccccc gacgctgtcg tcaccttgct gaccacggtt cgtgcgacca
     3961 cgaaggggcg ggtcatcgtg gtgctcgggt gcggcggtga tcgcgaccgg gcgaagcggc
     4021 cattgatggg cgccgccgcc gcgcgcctcg ccgacgttgc gatcttgacg agcgacaatc
     4081 cgcggagcga agaacccgag cagatcgtgg tcgcgctgct tgaaggcgca atgggcgtcg
     4141 cggctgcgca gcgcggtgag gtgatcgtcg aactcgatag gagggctgcg atcgccatcg
     4201 ccgtcgagcg ggctcgaccg ggtgacgtgg tcgtcgtcgc aggcaaaggg catgagcaag
     4261 ggcaggaggt cggcggcgtg gtgcatccgt tcgatgatcg cgccgtgctt cgtgccgagc
     4321 tcgcggccca cgcacacgcc gcgggtacct catgatcgct ctcacgatcg ccgagatcgc
     4381 gcagctgtcc aacggcacgg tttcaccgtt cgctgagccg accacgctgg ttgctggccc
     4441 ggtcgttgcc gactcgcggc tggtcgtgcc aggcgctctc ttcgtcgcga taccaggtga
     4501 gcgagtcgac gggcatcagt tcgctgcgca agcaatggcc gacggggcgc gtgccgtgct
     4561 ggcgagccgg ccagtgaacg tgcccgcggt ggtggtcgac gacacggttg caggccttgg
     4621 tgttctcgcg cgtggctatt tgaatcgtct agcagctcac gttggcccga cggtgatcgg
     4681 catcaccggg tcgagtggca agacgagtac gaaagacatc gtcgcgcaac tgctgtctaa
     4741 ccatggcccg accgtggcgc ccgccgggtc tttcaatacc gaagtcgggc tgcccttgac
     4801 cgtgctgcgg gccgacgccg ccacccgcta tctcgtgctc gaactttccg cgcggggcct
     4861 tggtcacatc gcctacctgt gtggcatcgc gcggccgagc atcggagtcg tgctcaacgt
     4921 cggtgccgcg catctcggcg agttcggcag ccgtgacgcg gtggcgcaag caaaaggtga
     4981 gctggtcgag gcgctgccgg ccgacggggt tgcgattctc aacgccgacg acgccgcggt
     5041 tgctgcgatg cgattgcgca ccgacgcgcg cgtggtgaca ttcggtgtcg aggcagctgc
     5101 agatgtgagt gccgtagaca ttcgcctcga cgaactggca cgaccggcgt ttcgcatcgt
     5161 caccgccgcc ggcgaggtcg aggtttcgct ggcattgcat ggggcgcatc acgtgctcaa
     5221 cgcgcttgcg gcggccgccg tcgctctcga agtcggcgtc gacg
//
LOCUS       DASWVF010000039         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_230436, whole genome shotgun sequence.
ACCESSION   DASWVF010000039 DASWVF010000000
VERSION     DASWVF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230436"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGD55_00670"
     CDS             complement(<1..360)
                     /locus_tag="VGD55_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868887.1"
                     /translation="MSALVIRDLHVVVNAGTTGEAGALAEEPREILRGVDLTVNAGET
                     HAIMGPNGSGKSTLAYSIAGHPKYTITKGSVTLDGQDVLAMKVDERARAGLFLAMQYP
                     VEVAGVSVSNFLRTAATA"
     gene            complement(357..725)
                     /locus_tag="VGD55_00675"
     CDS             complement(357..725)
                     /locus_tag="VGD55_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0868888.1"
                     /translation="MTTRYVKVGERAQLPAPRGVLRVELEGEPVAIICTDGGSYSAIS
                     DVCSHAEVALSDGDIDGTTVECWLHGSRFDVRSGKPLGPPATRSVPVYDIKFDGDDVY
                     LSAAPSEISSTSLDNQESVR"
     gene            complement(722..>1752)
                     /gene="sufD"
                     /locus_tag="VGD55_00680"
     CDS             complement(722..>1752)
                     /gene="sufD"
                     /locus_tag="VGD55_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0868889.1"
                     /translation="QEIWRFAALPRLRALFAGPDADGPLAYSWSADAPVQVVPIGDSR
                     VGAVHTPFDRLSAIALSKATEALVVTVAGDAVDPIVVGLKGTGGVAYGHVIVDIAPHA
                     TGTVVLDHVGTAQYAANVEVNVGDGASVTLVSIQDWDDDAVHASAHTVLVGRDAKVRH
                     INISFGGSAVRVTPLVKFAGPGGDAEMLGLFFADAGQHIDNRLLVDHAMPHCKSRVNY
                     RGALQGAGARSVWTGDVIIRASGIGTDTYEVNRNLLLTDGARADSVPNLEILTGEVVG
                     AGHASATGRFDDEQLFYLQARGIPADQARRLIVRGFFADVIERIQVPELVERLLATVD
                     RELDSSMS"
BASE COUNT          312 a          573 c          584 g          283 t
ORIGIN      
        1 cgcggtcgcc gcggtgcgca ggaagttcga caccgataca cccgcgactt cgacggggta
       61 ttgcatcgcg aggaaaaggc cggcgcgagc acgctcgtcg accttcatcg cgagcacgtc
      121 ctggccgtcg agggtgaccg agcccttggt gatggtgtac ttcgggtggc cggcgatcga
      181 gtacgccagt gtagatttgc cggaaccatt cgggcccatg atggcgtgcg tctcgcctgc
      241 gttcaccgtg aggtcgacgc cgcgcaggat ctcgcggggt tcctcagcca gcgcgccggc
      301 ctcgccggtg gtgccggcgt tgacgacgac atgcaggtcg cggataacaa gcgcgctcat
      361 cgcacggact cctgattgtc gagagaagtg gaggaaatct ccgaaggggc agcactgagg
      421 taaacgtcgt cgccatcgaa cttgatgtcg tacacgggaa ccgatcgagt cgcaggcggg
      481 ccaagcggtt tgccgctccg cacgtcgaag cgcgagccgt gcagccagca ctcgacagtc
      541 gtgccgtcga tgtctccatc ggacaacgca acttctgcgt gcgagcacac gtcggagatt
      601 gccgaatagc tgccgccgtc ggtgcagatg atcgcgaccg gctcgccttc aagctcgacg
      661 cgtaacaccc cacgcggcgc gggtagctgg gcgcgctcgc cgaccttgac gtagcgggtg
      721 gtcacgacat gctgctatcg agttcgcggt cgacggttgc cagcaaccgc tcgaccagct
      781 cgggaacctg gatccgctcg atcacgtcgg cgaagaaccc gcgcacgatc aagcgacgtg
      841 cttggtcggc cggaatcccg cgcgcctgca ggtagaagag ctgctcgtcg tcgaatcgcc
      901 cggtggcact cgcgtggcct gcacccacaa cttcaccggt caagatctcg aggttcggca
      961 ccgagtcggc acgtgcgccg tcggtgagga gcaggttgcg gttgacctcg taggtgtcgg
     1021 taccgatgcc gctcgcgcgg atgatcacgt cgccagtcca gaccgaacgc gcaccggcac
     1081 cctgcaacgc gccgcggtag ttgacccggc tcttgcaatg cggcatcgca tggtcgacca
     1141 gcaggcggtt gtcgatgtgc tgacccgcat cggcaaagaa gaggcccagc atctcggcgt
     1201 cgcctcctgg accggcgaac ttgacgagcg gcgtgacgcg cacggcgctg ccaccaaagc
     1261 tgatgttgat gtgccgcacc ttcgcatcgc ggccgacaag cacggtatgc gccgacgcgt
     1321 ggacagcgtc gtcgtcccag tcttggatgc tgaccagggt gaccgatgcg ccgtcgccga
     1381 cgttgacctc gacgttggcg gcgtactggg cggtgccgac gtggtcgagc acaaccgtgc
     1441 cggtcgcgtg gggcgcgatg tcgacgatga cgtgaccgta cgcgacgccg ccagtgcctt
     1501 tcaggccgac gacgatcgga tcgaccgcgt caccagcaac tgtgacgacc agcgcctccg
     1561 tcgccttcga taacgcgatg gctgacaacc ggtcgaacgg ggtgtgcacc gcaccgaccc
     1621 gcgaatcacc gatcggaacc acctgtacgg gcgcgtccgc gctccacgaa taggccagcg
     1681 gcccgtcggc gtcaggcccc gcgaacagcg cacgcaggcg aggtagcgcg gcgaaacgcc
     1741 agatctcttg ac
//
LOCUS       DASWVF010000040         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_383245, whole genome shotgun sequence.
ACCESSION   DASWVF010000040 DASWVF010000000
VERSION     DASWVF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383245"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGD55_00685"
     CDS             <1..190
                     /locus_tag="VGD55_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868890.1"
                     /translation="VTRFVVELDAGGELVALQQNLETSSSDVLAFRDGEWVWRGVRLT
                     SSQCWIPRASRPRVAFES"
     gene            201..1409
                     /locus_tag="VGD55_00690"
     CDS             201..1409
                     /locus_tag="VGD55_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0868891.1"
                     /translation="MRHTKAFSVTALAATAVLLISSCSSSKSNSGSAAASGSRAAPPS
                     EHMLNTLGKGEGALNIIVWAGYAEDGSDDKTVDWVHPFEQTTGCKVNAKVGNTSDEMV
                     TLMKTGQYDGVSASGDATLRLIYGGDVAPVNTSLVPNYATISTFLKDQPWNSVNGQMY
                     GIPHGWGANVLMWNPAVVKTAPTSWSSVFDPNSPYKGKITAYDSPIYIADAALYLEKT
                     QPNLKITNPYALDQTQFNAAISLLKAMKSNWSRPWAAYTDEVQDFESGATAIGTAWQI
                     NANLINGDKKVTVDTTVPAEGATGWSDTWMVSSKAAHPNCMYEWMNWITSPDTNAAVA
                     QWFGEAPAQTKACAIAAAGFCDQFHALDAAYASKISYWTTPTTQCLDGRGNICVDYAQ
                     WTQAWTTIKG"
     gene            1406..2308
                     /locus_tag="VGD55_00695"
     CDS             1406..2308
                     /locus_tag="VGD55_00695"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868892.1"
                     /translation="MTAPSTPTAGAPAARRLSAFLYRHERLRLAGLLTPPVLWLVVLY
                     LGSLGVMLVSSLWSVDSFTGDLVKKTSFANYHILFTQSVYRTVAIRTVGIAIGVTLID
                     LVLALPLAFFISKIVRSRRWRYALLIMVTMPLWASYLVKGYAWRIMFDKGGAIDWVLS
                     PIGAHGPGLGLTATVVALAYLWLPYMVLPVYAGLERLPESLLEASADLGASSMRTLRS
                     VVLPLLIPSIVAGSIFTFSLTLGDYIMVKIVGGTTQMFANIVYDNVGVAGNLPFAAAA
                     AVFPIVVVVAYLVAVRRTGALESL"
     gene            2313..3125
                     /locus_tag="VGD55_00700"
     CDS             2313..3125
                     /locus_tag="VGD55_00700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868893.1"
                     /translation="MTFSRRARVMFGTVACVALAFIYAPLIVVLVNSFNVSKVFSWPP
                     RGWTLSWWHKALVNDGVRTAVLTSVKAGLGATAIALVLGSMFSAAVVRYRWFGRDALS
                     LVVILPIALPGIVTGIALNSAFRTVLQPLGISFGLLTVVLGHATFCIVVVFNNVAARL
                     RRTGQSLQEASMDLGASSWQTFRFVTFPAVRSALVAGALLSFALSFDEIVVTSFTAGP
                     GIQTLPQWIFNNLFRPNQAPIVNAVAAALVVLFIIPVYLAQRLSDGGTGGRI"
     gene            3242..>3655
                     /locus_tag="VGD55_00705"
     CDS             3242..>3655
                     /locus_tag="VGD55_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0868894.1"
                     /translation="MRYRQIGRGTDAVTISAIGLGGMPLSVSGRPDEHQGVATIHAAL
                     DAGMTFIDTADAYCLTSADVGHGERLVATALASYGGDTTDILVATKGGHTRGPDGSWS
                     VDGRPEYLKRAAEQSLKALGVEAIGLYQFHRPDPRV"
BASE COUNT          597 a         1154 c         1192 g          712 t
ORIGIN      
        1 cgtcactcgg ttcgtggtcg agcttgacgc cggcggcgaa ctcgttgcgc tgcagcaaaa
       61 tctcgagacc tcgtcgtccg acgtgcttgc atttcgcgac ggcgagtggg tctggcgtgg
      121 cgtccggctc acgagttcgc agtgttggat tccgcgggca tcccgcccgc gggtggcgtt
      181 tgagtcatga gggagtaccc atgcggcaca cgaaggcgtt cagcgtcacc gcgcttgcgg
      241 cgactgctgt gttgctcatc agctcctgta gctcgtcgaa gtcgaactca ggttcggccg
      301 ccgcgagcgg ctcgcgggcc gcaccgccaa gcgagcacat gctgaacacg ctcggcaagg
      361 gcgagggcgc gctcaacatc atcgtctggg ccggctacgc cgaagacggt tctgacgaca
      421 agaccgtcga ctgggtgcat ccgttcgagc agacgaccgg ctgcaaggtg aacgcgaagg
      481 tcggcaacac ctccgacgag atggtcacct tgatgaagac cggtcagtac gacggtgtgt
      541 cggcatcggg tgacgccacc ctgcggctca tctacggcgg cgacgtcgcg ccggtgaaca
      601 cctcgctggt gccgaactac gcgacgatct cgacgttcct caaggaccag ccgtggaact
      661 cggtcaacgg tcagatgtac ggcatcccgc acggttgggg cgcgaacgtg ttgatgtgga
      721 acccagcggt ggtgaagacg gctccgacgt cgtggagttc ggtcttcgac ccgaactcgc
      781 cgtacaaggg caagatcacc gcgtacgact caccgatcta catcgctgac gctgctcttt
      841 acttggagaa gacccagccg aacctgaaga ttaccaatcc ctacgcactc gaccagaccc
      901 agttcaacgc tgcgatctcg ttgctgaagg caatgaagtc gaactggagc cggccgtggg
      961 cggcgtacac cgacgaggtt caagacttcg agagtggcgc gacggcgatc ggcacggcgt
     1021 ggcagatcaa cgccaacctg atcaacggtg acaagaaggt gactgtcgac acgacggtgc
     1081 cggcggaggg cgcgaccggc tggtcagata cctggatggt ctcgtcgaaa gccgcacacc
     1141 cgaactgcat gtacgagtgg atgaactgga tcacgtcgcc cgacaccaac gccgctgtcg
     1201 ctcagtggtt tggtgaggcg ccggcgcaga cgaaggcgtg cgcgatcgca gcggccggct
     1261 tctgcgacca gttccacgct ctcgacgccg cctacgcgtc gaagatctcg tactggacga
     1321 cgccgaccac gcaatgcctc gacggtcgcg gcaacatatg cgtcgactac gcgcagtgga
     1381 cgcaagcctg gacgacgatc aaggggtgac cgctccttca acaccgacgg cgggagcgcc
     1441 ggcagccaga aggctgtcgg cgttcctgta ccgccacgaa cggctgcggc tggcgggtct
     1501 gttaaccccg ccggtgctgt ggctcgtggt cctgtatctg ggatcgctcg gtgtcatgct
     1561 cgtgagttcg ctgtggagcg tcgacagttt taccggcgac ctcgtcaaga agacgtcgtt
     1621 cgcgaactac cacatcttgt tcacccaaag tgtctatcgc acggtcgcga tccgcacggt
     1681 cggcatcgcc atcggcgtca ccctcatcga cctggtgctc gcgctgccgc tggcgttctt
     1741 catctccaag atcgtgcgca gcaggcggtg gcggtacgcg ctgctcatca tggtcacgat
     1801 gccgctgtgg gcgtcgtacc tggtgaaggg ctacgcctgg cgcatcatgt tcgacaaggg
     1861 gggcgcgatc gactgggtac tttcgccgat cggcgcgcac ggcccgggtc tcggcctgac
     1921 ggcgacggtg gttgctctcg cctatctgtg gctgccctac atggtgttac ccgtctacgc
     1981 cggtctcgag cggttgccgg agtcgttgct cgaggcatct gctgaccttg gcgcatcatc
     2041 gatgcgaacc cttcgatcag tcgtgctgcc actgttgatt ccctcgatcg tcgccggctc
     2101 gatcttcacg ttctcgctca cgctcggcga ctacatcatg gtgaagatcg tgggcggcac
     2161 gacgcagatg ttcgccaaca tcgtctacga caatgtcggg gttgcgggca acctgccgtt
     2221 cgcggccgcg gcagcagtct tcccgatcgt cgtggtggtc gcgtatctcg tcgccgtgcg
     2281 ccgcaccggc gctctcgaga gcttgtaaga ccgtgacgtt cagccgccgc gccagggtga
     2341 tgttcggtac ggtcgcgtgt gtcgcgttgg cgttcatata tgcgccactg atcgtcgtcc
     2401 tggtcaattc tttcaatgtt tccaaggttt tctcctggcc accgcgcggg tggacgctgt
     2461 cgtggtggca caaggcgctg gtcaatgacg gcgtgcgaac ggccgtgctg acaagtgtca
     2521 aagcaggtct tggtgcgacc gcgatcgcgc tcgtcctcgg ctcgatgttc tcggccgccg
     2581 tggtgcgcta tcggtggttc ggccgcgacg cgttgagcct cgtcgtcatc ttgccgatcg
     2641 cgctgcccgg catcgtcacg ggtatcgcgt tgaactctgc gttccgcacg gtgctccagc
     2701 ctctgggcat ctcgttcggg ttgctcacgg tcgttctcgg tcacgcgacg ttttgcatcg
     2761 tggtcgtgtt caacaacgtc gccgcgcgac tacgccggac cggccagtcg ttgcaggagg
     2821 cgtcgatgga tctcggcgcg tcgagctggc agacgtttcg cttcgtgacg ttccccgcgg
     2881 tgcggtcggc gctggtcgct ggcgcgctgc tgtcgttcgc gctgtcgttc gacgagatcg
     2941 tggtgacctc gttcacggcg ggccctggca tccagacgct gccgcagtgg atcttcaaca
     3001 acctgttccg gccgaaccag gcgccgatcg tcaacgccgt cgcggcggcc ctggtcgtgc
     3061 tgttcataat cccggtctac ctcgcgcagc ggctgtcgga cggcggcacc ggcggccgca
     3121 tctgagcgct ctcaccagaa gttcaccgaa atgatcggaa tcagatggtg tccgatgcgt
     3181 tgaggatggg caggtgtcgg aaggcacgtt cagaccacga gcttgagacg ggatttcagt
     3241 catgcgctat cgccagattg gacgaggaac cgacgcggtc acgatcagcg cgattggcct
     3301 cggtgggatg cctttgtcag tttcggggcg cccggacgag caccagggcg tcgccacgat
     3361 ccacgccgcg ctcgacgccg gcatgacctt catcgacaca gctgatgcct actgcctcac
     3421 gtctgccgat gtgggccacg gcgagcggct ggtcgcgacc gcgctcgcct cctacggagg
     3481 cgacacgacc gacatcctgg tggcgaccaa gggcggtcac actcgcggcc ccgacggatc
     3541 ctggagcgtc gatggccggc ccgagtacct caagcgggcg gccgagcagt cgctgaaggc
     3601 gctgggcgtc gaggcgatcg ggctctacca gttccaccga cccgacccgc gcgtt
//
LOCUS       DASWVF010000041         5303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_281382, whole genome shotgun sequence.
ACCESSION   DASWVF010000041 DASWVF010000000
VERSION     DASWVF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281382"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /locus_tag="VGD55_00710"
     CDS             complement(<1..1885)
                     /locus_tag="VGD55_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868895.1"
                     /translation="MSSSAFRYGPWHEGPDPLAPPYDIAGALDELGDAVLDGANTADA
                     LRDLLRRGAPGLRGLEEMLRRVREQRRQLRSRGRLDGTLEQARALLDKAIGEERAALF
                     PDPSDDARLRETELDTLPSDPARAIRALAEYDWRSDQARATYEELKELLREEVLDSQF
                     RGMKQAMKQIQSGSPESAAAMQAVKDMMADLNEMLAADARGEHTQDDFDQFMAKHGQF
                     FPDNPANLEELVDSLARRAAAMERMLASMTPEQRDELAALMAQSMEDMGLASEMARLN
                     DALRTRRPDLDWTGRTRMKGNQPLGMSDGTTALEELADLEELESTLGQEYPGASLEDV
                     DEEAIRRALGRQAVDDLRQLREVERELERQGYLRRDGGRLELTAKAVRRLGATALRRV
                     FASLAGSTRGDHDVPDAGSAGELTGSSRAWQFGDEQPLDVVRTVSNAIRRNGASTSGV
                     KLHVDDFEVVETERRTSAAVCLLVDLSWSMTLRGTWGVAKSTALALHSLVSTRFPQDA
                     LQVIGFSNYARVLQPAELAGLDAEMVQGTNLQHALLIAGRFLDRHPEHEPVVLVVTDG
                     EPTAHLLPSGDYTFDWPPSPETLAVTLAEADKMTRRGATLNVFMLAQDQGLVDFVEKL
                     AKRN"
     gene            complement(1887..3257)
                     /locus_tag="VGD55_00715"
     CDS             complement(1887..3257)
                     /locus_tag="VGD55_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0868896.1"
                     /translation="MTLGALRASGHVHKPVKVEIRDNLLARLRSGEPRFPGIVGFDET
                     VLPHVERALLAGHDFVLLGERGQGKTRLIRTISGLLDEWTPVVDGCEINDHPYAPVCT
                     RCLRLAAELGDDLPVDWKNRDERYGEKLATPDTSVGDLIGDVDPIKVAEGRTLGDPET
                     VHYGLVPRFNRGIFSVNELPDLAERIQVSLLNVLEERDVQIRGYALRLPLDVLLIASA
                     NPEDYTNRGRIITPLKDRFGAEVRTHYPLRLTDEIDLITQEADLTAVVPPYLLDVVAR
                     FTRLVRESTAIDQRSGVSARFAVAGAETVAASALRRAALTGEADAVARVCDLPGIVDS
                     LQGKVEFEVSEEGRERDVLVHLLRRAVAETFRAKLGNADLSGLLARFEEGATVETGEL
                     VPASELLKRIGQVPGLAKVLERLGDDGGENPGRAAAGIEFALEGLYLTRRLSKDEVPG
                     RTVYGG"
     gene            3393..4154
                     /locus_tag="VGD55_00720"
     CDS             3393..4154
                     /locus_tag="VGD55_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868897.1"
                     /translation="MKRTGTVAHGNVPATAAASAAIAAAIMLGGCAAPGSASARSGVI
                     AVPSSAEPATTSSEGPAPASSADPAPASSADPAPASSGDPGLGDTRPPCPFAGFQLML
                     GPNASERAGTDALLRKPFGDVGVNFGPQMPGATMLWARLDVVVTDATPIPGNPTADPS
                     AFRAAEDARPDSFRKMMAGTDPHVARVTLKPFAPGPFVLPAALLNALPAGVASYTVYL
                     VESVDESVCFGATPDPSLVDSDEGDGYSVIAELAP"
     gene            4234..>5303
                     /locus_tag="VGD55_00725"
     CDS             4234..>5303
                     /locus_tag="VGD55_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0868898.1"
                     /translation="MARFGDGLAVDADLVRAAEAASIAARTPLGGLSPDLACVFVCGS
                     DPTQVEEALQRAAGRLRARTVIGCSASGVIGAAQGIEATSAVSVWAAVLPGVRVRAFH
                     LEVIRTSGGMAILGLPEGEDADEVAVVLADPYSFPADGFVEQANEALGLPLVGGLATG
                     NRGAGSTRLLIDGRVVDRGAVGVVLGGPVGAQAMVSQGCRPVGPSMTVTAAEGNLLLE
                     LAGVPAITKLEQVISSLSSNDQALATAGLQVGIAMDEYIEEHERGDFLVRGILGLDKT
                     RGAIAIADVVPVGRTVRFQVRDAASADDDLRAMLVELRSGFSAFEGALLFSCNGRGSN
                     LFTSADHDVLAIRQGLATSGIAG"
BASE COUNT          785 a         1771 c         1837 g          910 t
ORIGIN      
        1 cgttgcgttt ggcgagcttc tcgacgaagt cgacgagacc ttggtcttgg gcgagcatga
       61 agacgttgag tgtcgcgccg cggcgggtca tcttgtcggc ttcggcgagc gtgacggcca
      121 gcgtctctgg tgatggcggc cagtcgaagg tgtagtcgcc gcttggcagc aggtgcgcag
      181 tcggctcgcc gtcggtgacg acgagcacga ccggttcgtg ctccgggtgc cggtcgagga
      241 atcgtccggc aatcaacagc gcgtgctgaa ggttggtacc ttgcaccatc tcggcgtcga
      301 ggccggcgag ctcggcgggc tgtaaaaccc ttgcatagtt ggagaatccg atgacttgca
      361 gcgcgtcttg agggaatcgg gtgctgacga gggagtgcaa agcgagtgcg gtcgacttcg
      421 cgactcccca cgtgccgcgt aacgtcatcg accacgacag gtcgacgagc aagcagacgg
      481 cggccgacgt gcggcgctcg gtttcgacga cctcgaagtc gtcgacgtgc agtttcacac
      541 ccgacgtcga tgccccgttg cgacggatcg cgttcgacac cgtccgcaca acatcgagcg
      601 gctgctcgtc gccgaattgc cacgcccgcg aagaacccgt gagctcaccg gccgaccctg
      661 cgtccggcac gtcgtggtcg ccgcgggtgc tgcccgccag cgacgcgaac acccgacgca
      721 gtgcggtcgc accgagtcgc cgcacggctt tcgcggtcag ctcaaggcgc ccaccgtcgc
      781 ggcgcaggta cccctgccgt tcgagctcgc gctcgacctc acgcagttgg cgcaggtcat
      841 cgaccgcctg ccgcccgagc gcacgccgga tcgcctcttc gtcgacgtcc tcgaggctgg
      901 cgcccggata ctcctgtccg agcgtcgact cgagttcctc gagatcggct agctcctcaa
      961 gggcagtcgt cccgtcggac atgcccagcg gttggttgcc cttcatgcgg gtgcggccgg
     1021 tccaatcaag gtcgggacgt cgagtgcgta gcgcgtcgtt caagcgcgcc atctccgagg
     1081 caagacccat gtcttccatc gactgcgcca tcagcgcagc aagttcgtcg cgctgctccg
     1141 gggtcatcga cgcgagcatg cgttccatcg ctgccgctcg ccgcgcaagg gagtcgacaa
     1201 gctcttcgag gttggccggg ttgtcgggga agaactgccc gtgcttggcc atgaactggt
     1261 cgaagtcgtc ctgcgtgtgc tcgccgcgcg catcggcggc gagcatctcg ttgaggtcag
     1321 ccatcatgtc cttgacggcc tgcatcgcgg cggccgattc gggtgagccc gactggatct
     1381 gcttcatggc ttgcttcatg ccgcggaact gactatcgag cacctcctcg cgcagcagct
     1441 ccttcagttc ctcgtaggtg gcacgggcct ggtctgaccg ccagtcgtac tcggccagcg
     1501 cgcgaatagc ccgagccgga tcggatggca gcgtgtcgag ctcggtttcc cgcagccggg
     1561 cgtcgtcgga cgggtcggga aagagcgcgg cgcgctcttc accgatcgcc ttatcgagca
     1621 gggcgcgggc ctgttcgagc gtgccgtcga ggcggccgcg cgatcgcaac tgccgccgtt
     1681 gctcacgtac ccggcgcagc atctcctcga ggccgcgcag ccctggcgcg ccccggcgca
     1741 gcaggtcacg cagcgcatcg gcggtattcg caccgtcgag caccgcgtcg ccgagttcgt
     1801 cgagcgcccc cgcgatgtcg tacgggggtg cgagcggatc aggaccttcg tgccatgggc
     1861 cgtagcgaaa cgcggaggag ctcacgctag ccgccgtaca ccgtgcggcc cggaacctcg
     1921 tctttgctca accgtcgcgt gaggtacagg ccttcgaggg cgaactcgat gccggccgcc
     1981 gcgcgcccgg ggttctcgcc accgtcgtcg ccgagccgct cgagcacctt ggcgagccct
     2041 ggcacctgac cgattcgctt gaggagctcc gaggccggga caagctcgcc ggtctcgacg
     2101 gttgcgcctt cttcgaaccg cgcaagcagc ccgctgaggt cggcgttgcc gagcttcgcg
     2161 cgaaatgtct cggccactgc tcgccgcaag aggtgcacca agacgtcgcg ttcgcgtccc
     2221 tcttcgctga cctcgaattc gaccttgccc tgaagcgaat cgacgatgcc gggcaggtcg
     2281 cagactcgcg ccacggcgtc cgcctcgccc gtcaacgcag cccgtcgaag tgccgacgca
     2341 gcaaccgttt cggcacccgc aacggcgaac cgtgcagaca ccccggaccg ctgatcgatg
     2401 gccgttgact cgcgcaccag ccgggtgaat cgcgcaacga cgtcgagcag gtacggcggg
     2461 acgacggcgg tgaggtcggc ttcttgggtg atgaggtcga tctcgtcggt gaggcgcaac
     2521 ggatagtgcg tacgcacctc ggcgccgaac cggtctttca gcggcgtgat gatgcggcca
     2581 cggttggtgt aatcctcggg gtttgcactc gcaatcagca ggacgtcgag tggcagccgc
     2641 agtgcatatc cgcggatctg cacatcgcgc tcttcgagca cgttcagcag cgacacttga
     2701 atgcgctcgg cgaggtcggg cagctcgttg accgagaaga tgccgcggtt gaatcgcggc
     2761 accaagccgt agtgcaccgt ctcgggatcg ccgagcgtgc ggccctcggc aaccttgatg
     2821 gggtcgacgt cgccgatcag gtcaccgacg ctggtgtcgg gggtcgcgag cttctcgccg
     2881 taccgctcgt cgcggttctt ccagtcgacc ggcaggtcgt cgccgagttc ggccgcgagc
     2941 cgcaggcagc gcgtgcacac cggggcgtac gggtggtcgt tgatctcgca gccgtcgacg
     3001 accggggtcc actcgtcgag cagcccgctg atcgtgcgga tcaggcgcgt cttgccctgc
     3061 ccacgctcgc ccaggagcac gaagtcgtga ccggccagca gcgcgcgctc gacatgcggc
     3121 aggaccgtct cgtcgaaccc gacgatgcca gggaatcgcg gttcgcccga ccgcagccgg
     3181 gcgagcaggt tgtcgcggat ctcgaccttg acgggtttgt gcacatggcc cgaggcgcga
     3241 agcgcgccga gtgtcacgat cgatgggcgg gacgtcgagg ggctggccgc tggggccggc
     3301 ggcggacgcg cagacttggg tgaaggagat tgaggcacct tttgacccta cgtcgcactg
     3361 aaccgttacg tcggaccggg cgtccaagcg gcatgaagcg caccggcact gtcgctcacg
     3421 ggaacgttcc agccactgcg gccgcgtcag ccgcgattgc tgcagcgatc atgctcggcg
     3481 ggtgcgctgc ccccggctcc gcctcggctc gttcgggggt catcgcggtg ccgtcgtcgg
     3541 ctgaaccggc caccacctca tcggaaggcc cagcgcctgc atcttcggct gaccccgcgc
     3601 ccgcgtcgtc ggctgacccc gcgcccgcgt cgtcgggcga tccggggctc ggcgacaccc
     3661 gcccgccgtg cccgtttgcc gggttccagc tgatgttggg gccgaacgcg tcagagcgcg
     3721 ccgggaccga cgcgctcctg cgcaagccgt tcggcgatgt cggcgtgaac ttcggcccgc
     3781 aaatgcccgg cgccaccatg ctgtgggccc gtctcgatgt ggtggtcacc gacgcgacgc
     3841 caattccggg caaccccacc gccgacccga gcgcgttccg ggcggcggag gatgcccggc
     3901 ccgattcgtt tcggaagatg atggccggta ctgacccgca tgtcgctcgc gtcacgctca
     3961 agccgttcgc acccggccca ttcgtgttgc cggccgcctt gctcaacgcg ctgccggccg
     4021 gagtcgcgtc gtacaccgtc tatctcgtcg aatccgtcga cgagagtgtg tgttttggcg
     4081 ccacacccga tccgtcgttg gtcgactcag acgagggtga cgggtacagc gtcatcgcgg
     4141 agctggcgcc ctgacgcgta gacccgcgga agaccacccg gatgcgctac cactacgcat
     4201 gacagcggtc gccccagccg ggaggataga cgcgtggcgc gatttggtga cgggctcgcc
     4261 gtcgatgcag acctggtgcg tgccgccgag gctgcgtcga tcgctgcgcg cacgccgctc
     4321 ggcgggctga gcccagacct ggcctgcgtg ttcgtatgcg gatccgaccc cacccaggtc
     4381 gaggaagcac tccagcgggc agcaggcagg ctccgcgccc gcacggtgat cggatgcagt
     4441 gcctctggcg tcatcggcgc agctcagggc attgaagcca cgagtgcggt cagtgtgtgg
     4501 gcggcggtac tccccggcgt ccgggtaagg gcttttcatc ttgaagtgat ccgcacgtca
     4561 ggcggcatgg cgatcctcgg tctacccgag ggggaggacg ccgacgaggt tgcggtcgtg
     4621 ctcgccgacc cctactcgtt cccggctgac ggcttcgtcg agcaggcgaa cgaggcgctc
     4681 ggcctgccgc tcgtaggcgg cttggcgacc ggaaaccgcg gtgccggctc gacgcgactg
     4741 ttgatcgacg gacgggtcgt cgaccgcggc gccgtcgggg ttgtgctcgg cggccccgtc
     4801 ggggcgcagg ccatggtgag ccaaggctgc cggccggtgg gtccctcgat gacggtcacg
     4861 gccgccgagg gcaacttgct gctcgagctc gccggagtgc ccgcgatcac caagctcgag
     4921 caggtgatca gctcgctgtc gtcgaacgac caggctctcg cgacggccgg tctgcaggtg
     4981 ggcatcgcga tggacgagta catcgaggaa cacgagcgcg gcgattttct ggtgcgcggc
     5041 atcttgggcc tcgacaagac gcggggcgcg atcgcgatcg ccgacgtggt gccggtcggt
     5101 cgcaccgttc gtttccaggt tcgcgacgct gcgtcggcgg acgacgattt gcgggcgatg
     5161 ctcgtcgagc tccggagcgg gtttagtgcg ttcgagggcg cgctgttgtt ttcctgtaac
     5221 ggcaggggtt cgaacctgtt tacctcggcc gaccatgatg tgctggcgat aaggcaaggc
     5281 ctcgcgacgt cggggattgc ggg
//
LOCUS       DASWVF010000042         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_281451, whole genome shotgun sequence.
ACCESSION   DASWVF010000042 DASWVF010000000
VERSION     DASWVF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281451"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VGD55_00730"
     CDS             complement(<1..1779)
                     /locus_tag="VGD55_00730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0868899.1"
                     /translation="MATTKIPGVEELLDLAVAGIGGEARDGQLTMARAVEHAMRSGDH
                     LLVQAGTGTGKSLAYLVPAIRHALATDPPVPKPKIDGANDELPPPRGPVVVATATIAL
                     QRQLIERDLPRLAESLAPALPVTPVFAILKGRRNYLCLQRLHAGPDAEQDGLFDDVGP
                     TSSGSGATGPTSALGRDVVRITEWANTTDTGDRDELVPGVSGRAWSQVAVSSRECLGA
                     TNCPYGADCFAESARMRAMRADVVVTNHALLAIDALSDVDVLPEHDVVVIDEAHELVD
                     RVTGVATEELTGGIVERAAARCHRLAAEESAQLAEAAAALSDVLALSPIGRLSPLPTS
                     LVEVISLVRDAARATLGVMATPDTATKAASAEAAADVEVVRRQARVAVEAVFDTAERL
                     LEDSDDDVIWTASEDRRGVVLRVAPLHVAELLRKALFNQRTVVLTSATLTLGGRFEPV
                     AIGLGLSNASSDEQPGWIGLDAGSPFDHGRQGILYVAKHLPPPGRDGTPPETMTELAE
                     LIEAAGGRTLGLFSSMRAAVDAAEALRTLVDVPILCQGEDSTSELVRQFAAQPTSCLF
                     GTLSLWQGVDVPGASCQLVVVDRIPFP"
     gene            1888..>2206
                     /locus_tag="VGD55_00735"
     CDS             1888..>2206
                     /locus_tag="VGD55_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868900.1"
                     /translation="MDMYVVKLVFAIGWAAFWIYWFVAALSTKRGHLAWSRELRVRAV
                     IVVLAILLVRFGAFRYHHANSDPWRTAIGLVLFGAGLAFSIWARLHLGRNWGSPMSRK
                     NEPE"
BASE COUNT          390 a          742 c          708 g          366 t
ORIGIN      
        1 gggaaacgga atgcggtcga caaccaccaa ctggcacgac gcacccggca cgtcaacacc
       61 ctgccataac gacaaggtgc cgaagagaca cgacgtgggc tgcgcggcaa actgacggac
      121 aagttccgac gtcgaatcct cgccctgaca aagaatgggg acgtcgacca gtgtgcgcaa
      181 tgcctcagct gcatcgacgg cggcgcgcat cgacgagaag agcccaagcg tgcggccgcc
      241 tgcagcctcg atgagctcgg ccaactcggt catcgtttcc ggcggcgtgc cgtcgcggcc
      301 tggaggcggc aaatgcttgg ccacatagag aattccctgc cgcccgtggt cgaacggcga
      361 ccctgcatcg agcccgatcc agcctggctg ttcgtcgctc gacgcgttcg acagaccgag
      421 tccgatggcg accggctcga agcgtccgcc caaggtcaac gttgctgacg tcaacacgac
      481 cgttcgctgg ttgaacaacg ccttgcgcaa gagttcggcg acgtgcagcg gtgcaacccg
      541 cagaaccact ccgcgccgat cttcggacgc ggtccagatg acgtcgtcgt cagagtcctc
      601 gaggaggcgc tcggcagtat cgaagactgc ctcgaccgcg acgcgtgcct gtcgccgtac
      661 gacctctacg tccgcggccg cttcggcgct cgctgccttg gtcgccgtat cgggggtagc
      721 catcacgccg agcgttgccc gcgcagcgtc gcgaaccagc gagatcacct cgacaagcga
      781 ggtcggcagc ggcgacagcc tgccaatagg cgacaacgcc agcacgtcgg acagcgccgc
      841 ggctgcctct gcgagttgcg ccgactcttc ggccgcgagt cgatgacacc gagctgcggc
      901 ccgttcgacg atgccgccgg tcagctcttc ggtcgcgaca ccggtgaccc ggtcgaccaa
      961 ctcgtgggcc tcgtcaatga cgacgacgtc atgctccggc agcacgtcga cgtcgctcaa
     1021 cgcgtcgatg gcgagcaacg cgtggttggt gacaacgacg tcggcacgca tcgctcgcat
     1081 tcgcgccgac tcagcgaaac agtctgcgcc gtagggacaa ttggtcgcac cgagacattc
     1141 gcgcgacgac accgcgacct gactccacgc ccgcccgctg acaccgggga ccaactcgtc
     1201 acgatcaccg gtatcggtgg tgttcgccca ttcagtgatg cgcacaacgt cgcgaccgag
     1261 cgccgaagta gggccagtag cgcccgatcc tgatgatgtt ggtcccacgt catcgaacaa
     1321 gccgtcctgc tcggcgtccg gacctgcatg aagcctttgc aggcataggt aattgcggcg
     1381 ccccttcagg atcgcgaaga ccggcgtaac gggtagcgcg ggggcgagcg actcggcaag
     1441 gcgaggcagg tcccgctcga tcagctgccg ttgcaacgcg atcgtcgcgg tggccaccac
     1501 gactggtccg cgcggcggcg gcaactcgtc gttcgcaccg tcgatcttcg gcttgggaac
     1561 cggcgggtcc gtggcgagtg catggcgaat cgccgggacg aggtaagcga ggctcttgcc
     1621 cgtgccggtg cccgcctgca cgaggagatg gtcaccggag cgcatcgcgt gttccacggc
     1681 gcgcgccatg gtcagctggc cgtcgcgcgc ctcaccaccg atcccggcga cggcgaggtc
     1741 gagcagctcc tcgacgccgg gaatcttggt agtagccacg gctcgaaggg tagctggcgg
     1801 gccggtgccg gatcgggcgc acacccacag gctgcgcctc ccaggctgat cagttcgtgg
     1861 tacgccggcc gacattggtg cactgttatg gacatgtacg tggtcaagct tgtgttcgcc
     1921 atcgggtggg cggcattttg gatctactgg ttcgtcgccg ccctctccac caaacgaggg
     1981 cacctagcct ggtcgcgcga acttcgagtt agggcagtca ttgtcgtcct cgcgatcctc
     2041 ttggtccgct tcggagcctt ccggtaccac cacgccaact ccgatccgtg gcgcaccgca
     2101 atcggcctcg tcctattcgg cgccggattg gcgttctcga tctgggcccg cttacacctc
     2161 gggcggaact ggggtagccc gatgtctcgt aagaacgaac cggaat
//
LOCUS       DASWVF010000043         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_179598, whole genome shotgun sequence.
ACCESSION   DASWVF010000043 DASWVF010000000
VERSION     DASWVF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179598"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGD55_00740"
     CDS             complement(<1..209)
                     /locus_tag="VGD55_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868901.1"
                     /translation="MPPTVKLAAAVPSGAVALVVAITPGTGDPVIAKLGRGAAGDIES
                     FGADPDALLAVLTREKTKGNVGDAGA"
     gene            complement(340..507)
                     /locus_tag="VGD55_00745"
     CDS             complement(340..507)
                     /locus_tag="VGD55_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEY0868902.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVELSADEAS
                     ALGDALKNVVG"
     gene            671..1480
                     /locus_tag="VGD55_00750"
     CDS             671..1480
                     /locus_tag="VGD55_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0868903.1"
                     /translation="MSEFPAAAGRRHGVDLAIADAVGTVTIASDRGVLSTETKTALRD
                     ALNRVADDRAVRAVVLTGSGSTFCVGQDLREHAAALAADATAAWDTLGDHYIPIITAL
                     ATMPKPVIAAVNGTAAGAGAAMAFACDLRLVSDAAVFYLAFAGIGLSVDSGLSWTLQR
                     LVGFGRATDLLLRARPVAAADALALGLATEVVPAPDLAARAAVVAAELAAGPTVAFGA
                     MKQALAYSAAHGLPDALDMEAGMQTLAGTTEDHANAVRAFLDKQPPVFRGR"
     gene            complement(1484..>2050)
                     /locus_tag="VGD55_00755"
     CDS             complement(1484..>2050)
                     /locus_tag="VGD55_00755"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY0868904.1"
                     /translation="IVGPDGRRRCTWAHSAPEYIDYHDNEWGRAVHGDDAMFERLVLE
                     AFQSGLSFITILRKRDAFRKAFAGFSIEAVAQFDEADAQRLMSDAGIVRNRAKIAASM
                     ANARAAMAVPEGLSELLWSFAPTRRRAAPKDDADIPATSPESTAMAKELKRRGFQFVG
                     PTTAYAHMQATGIVNDHLATCYVRTLVS"
BASE COUNT          316 a          704 c          687 g          343 t
ORIGIN      
        1 gccccggcgt ccccgacgtt gcctttcgtc ttctctctgg tgagcaccgc gagcagcgca
       61 tccgggtctg caccgaagct ctcgatgtcg cccgctgccc cacgtccgag cttcgcgatg
      121 accgggtcgc cggtgcctgg cgtgatcgcg accacgagtg caacagcgcc actgggcact
      181 gcagctgcga gctttaccgt gggcggcacg catgcacctt tcgaagctgg cgtaggactc
      241 gaatctgggg gacaaccgag tcgacgctac ccctgacacg attgaggccc cgaacgaagt
      301 cccagttggg cttcgacggg gccgccaccg ctgggtcgat cagccgacca cattcttgag
      361 ggcgtcgcca agggcgctgg cctcgtctgc agacagctcg acgacgagcc ggccaccgcc
      421 ttcgagagga acgcgcatca cgatgccgcg accctccttg gtgacctcga gcggaccgtc
      481 gcccgtccgc ggcttcatgg ccgccattcg cgctcctgtt gtacgtccta cacgtcgagc
      541 ggtgaacgtc ggcacgtcga gcgccgaacc ccatcgcact gtcttaacca agattatcgt
      601 ggagagaggg ggttagaagg aaaccgtggc ggcgtccccg cccgggccag ggcagttccg
      661 aggaggatct gtgtccgaat tcccggccgc tgcgggtcgt cgccacgggg tcgatctggc
      721 catcgcggac gccgtgggaa cggtcacgat cgccagtgac cggggggtcc tctccaccga
      781 aaccaagacc gcgttacgcg atgcgctgaa ccgggtcgcc gatgaccggg ccgtgcgcgc
      841 ggtggtgctc accgggtcgg gaagcacgtt ctgcgtcggc caggacctca gggagcacgc
      901 cgccgcactc gccgcagacg ccaccgccgc ctgggacacc ttgggcgatc actacatccc
      961 gatcatcact gctttggcga cgatgccgaa gccagtcatc gcagcagtaa acgggacggc
     1021 ggcaggtgcg ggtgcagcga tggccttcgc gtgtgatctg cggctggtgt cggacgccgc
     1081 ggtgttctat ctcgcgttcg cgggcatcgg gttgtcggtc gactccgggc tgtcgtggac
     1141 cttgcagcgg ctggtgggtt ttgggcgggc caccgacttg ctgctgcgcg cacggccggt
     1201 ggcggcagcc gacgccttgg ctctcggcct ggcgactgaa gtggttcctg ctccggacct
     1261 tgcggcgcgc gctgcggtcg tcgccgccga gctcgccgcc ggcccgaccg tggcgttcgg
     1321 cgcgatgaag caggcgctcg cgtactccgc cgcacacggc ttgcccgacg ccctcgacat
     1381 ggaggccggc atgcagacac tggccggcac gacggaggat cacgccaacg cggtgcgcgc
     1441 gttcctcgac aagcagccgc ccgtcttccg cggccgctag ttctcaggaa acgagcgtgc
     1501 gcacgtagca ggtagccagg tggtcgttga cgatgccggt cgcctgcatg tgcgcgtaag
     1561 ccgtcgtcgg cccgacgaac tgaaaaccac gccgcttcaa ctccttggcc atcgcggtcg
     1621 actccgggga ggtggccggg atgtcggcgt cgtctttcgg agcagcccgc cgccgggtcg
     1681 gcgcgaacga ccacaacagc tcagacaatc cttcgggcac cgccatcgcg gcgcgcgcgt
     1741 tcgccatgct ggcggcgatc ttcgctcggt tgcggacgat gcccgcatcg gacatcagcc
     1801 gctgtgcgtc cgcctcgtcg aactgcgcaa ccgcctcgat cgagaacccg gcgaacgcct
     1861 ttcggaacgc gtcgcgtttg cgcaagatcg tgatgaacga caagcccgac tgaaaggcct
     1921 cgagcaccag ccgctcgaac atcgcgtcgt cgccgtgaac agcgcggccc cactcgttgt
     1981 cgtggtagtc gatgtattca ggagcgctgt gcgcccaggt gcaccggcgc ctgccatcgg
     2041 gtccgacgat
//
LOCUS       DASWVF010000044         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_128651, whole genome shotgun sequence.
ACCESSION   DASWVF010000044 DASWVF010000000
VERSION     DASWVF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128651"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGD55_00760"
     CDS             complement(<1..317)
                     /locus_tag="VGD55_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0868905.1"
                     /translation="MALDDIDQRILAALRDDARASFAEVGSQVGLSAPAVKRRVDKLR
                     ATGVITGFAAIVDPSALGWTTEAYVELYCQGRTSPAAIADAVSRHPEVVDACTVSGEA
                     DAL"
     gene            complement(317..1246)
                     /locus_tag="VGD55_00765"
     CDS             complement(317..1246)
                     /locus_tag="VGD55_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868906.1"
                     /translation="MTLTAGQLPLSRRHRPVGPLDLDATLAPHRRGRYDPCQRVGSHG
                     EHWRTWRTPLGPATVRLSANAGEIDAQAWGPGASWALDGVPALLGADDDWTGLELPAG
                     VLRDTRRRLNGLRLSASGRVFEALVPAVFEQLVTGIEARRGWTALLRKFGDPAPGPAP
                     EGMRVLPAASVLSAIPDWDWHRIGLDGARRRTLLVAASVATRLDEAASLDIETAFARL
                     TSLPGIGAWTAAEVVQRSHGAADVVSVGDYHLPNTVGFVLTGRPRTDDAGMLALLEPF
                     RPHRQRVVRLIEACGIAAPRFGARMALRDYRRI"
     gene            complement(1251..>1791)
                     /locus_tag="VGD55_00770"
     CDS             complement(1251..>1791)
                     /locus_tag="VGD55_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY0868907.1"
                     /translation="RDVIDTIPVGRPVSVERETFPQLLTGGATVMGYVDSAYWLDLGT
                     PAAFVQGSCDLVRGIAPSPALPGPIGDALVLAGATVAASAILSGGSCVSPGAVIGEDA
                     VVDGCVVFDDAVVEAGARLHASVVGRGARIGAGVVLDGAVIGDGAVIGAGNELLTGVR
                     VWPATVLAPGSVRFSSDAE"
BASE COUNT          325 a          644 c          586 g          236 t
ORIGIN      
        1 atcagcgcgt cggcctcccc cgacaccgtg cacgcgtcga ccacctccgg atgccgcgac
       61 accgcgtcgg cgatcgcggc cggcgacgtc cgcccctggc agtacagctc gacgtaggcc
      121 tcggttgtcc agcccagcgc cgacggatcc acgatcgccg cgaaaccggt gatgaccccg
      181 gtcgcgcgca acttgtcgac gcgccgcttg accgcgggcg cgctgagccc aacctggctg
      241 ccgacttcgg cgaagctcgc ccgcgcgtcg tcgcgcaacg ccgcgagaat ccgctggtcg
      301 atgtcgtcga gtgccatcag atacgccgat agtcgcgcag cgccatccgc gcaccaaatc
      361 gcggagcagc aataccacaa gcctcaataa gccggacgac gcgttgccgg tgtggtcgga
      421 aaggctcgag aagagcgagc atccccgcat cgtccgtgcg cggccggccg gtgagcacga
      481 agcccaccgt gttcggcagg tgatagtcgc caaccgacac gacatcggcg gcaccgtgac
      541 tgcgttgcac gacttcagcg gcggtccatg caccgatccc cggcaacgac gtcaggcgtg
      601 cgaaagctgt ctcgatatcg agagatgctg cctcgtcgag tcgggtggcg accgaagccg
      661 cgacaagcaa cgtgcggcgg cgagcaccgt cgaggccgat gcgatgccag tcccagtcgg
      721 gaatcgcgct caacaccgac gccgccggca acacccgcat gccctcaggt gccggacccg
      781 gcgcggggtc accaaactta cgcagcaacg ctgtccagcc tcgccgggcc tcgatgccgg
      841 tcaccagctg ttcgaaaact gccggcacca atgcttcaaa gacacggcca ctggctgaga
      901 gccgcaagcc gttgagccgc cggcgggtat cgcgcaagac gccggccgga agttcgaggc
      961 ctgtccagtc gtcgtcggca ccgagcagcg caggcacccc gtcgagcgcc cacgatgcgc
     1021 ctggtcccca cgcctgggcg tcgatctcgc ccgcattggc ggaaagccga acagtggcgg
     1081 ggccgagcgg cgtccgccag gttcgccaat gttcgccgtg ggaacccacc cgctggcagg
     1141 ggtcgtagcg gccgcggcgg tggggagcca acgtcgcatc gaggtcgagc ggcccgacgg
     1201 gccggtgacg gcgcgagagc ggcagctgcc cggcggtcag ggtcacgtgg ctattctgcg
     1261 tcgctcgaga accgtactga gccaggcgcg agcaccgttg ctggccaaac ccgcacgcct
     1321 gtcagcaact cgttcccggc accaatcacg gcaccgtcgc caatcactgc tccgtcgagc
     1381 acgaccccgg cgccgatcct ggcgccgcga ccgacgacgc tggcatgcaa acgcgcgcct
     1441 gcttcgacaa cggcgtcgtc gaagacgacg caaccgtcga cgacagcgtc ctcgccaatc
     1501 acggctccgg ggctgacaca actgccaccg ctcaggattg ccgacgcggc gacggtcgcg
     1561 ccggcgagta cgagcgcatc gccgatcggg cctggcaatg caggtgacgg tgcgatgcca
     1621 cgcacgagat cgcacgaacc ctgcacgaac gctgcggggg ttccgaggtc gagccagtag
     1681 gccgaatcga cgtatcccat gaccgtggcc ccgccagtga gcagctgcgg aaacgtctca
     1741 cgttcgaccg aaacgggtcg cccgacaggg atcgtgtcga tcacgtcgcg c
//
LOCUS       DASWVF010000045         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_357968, whole genome shotgun sequence.
ACCESSION   DASWVF010000045 DASWVF010000000
VERSION     DASWVF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357968"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VGD55_00775"
     CDS             complement(<1..1059)
                     /locus_tag="VGD55_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868908.1"
                     /translation="MNVPVLTAVTDAVWEADLVSAFERQDFGVIVVRRCVDLADLLAA
                     ASAGTARAVILSADLRRLDRDALARLATAGVAVIGLVARSSGGVQGDDAERRLRQLGV
                     RHVLAADAGPAVISAEVASAVAEGVLTGGLDPMTLGYAEPRSALPDVPASGSELFAQP
                     ASSAAFNLEAADAGALVAVWGPTGAPGRTTIAVTLATEAARLGCSTLLADADCYGGVV
                     AQVLGLLDESPGLAAAVRHANAGTLDDRTLATLARVVSPNLRVLTGIARAERWPELRP
                     AGLTAVYAMARRLCKLTVVDCGFGLEQDEELSYDTTAPRRNGSTLATLADADHVVAVG
                     TADPVGLQRLIRGLAELAQ"
     gene            complement(1056..1781)
                     /locus_tag="VGD55_00780"
     CDS             complement(1056..1781)
                     /locus_tag="VGD55_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868909.1"
                     /translation="MSDLPALPAVPAVRLKSPSWLDGRLIVGVVLVLLSVLVGAKIIA
                     SSDHYDAIWAASRDLAPGTTLVKADLLRVNVRFKDHGGVYISADGPSPVGRTTTQPLG
                     DGQLIPLAALPSSPPVPVRLVTIPVAKLHMPSGNDLHGVQVDLYVTPKAVGDAHAAPQ
                     LVLANVTIASTITDSALGGSNGSGVVLSVPLPYVDAVVAAAQQGAVDLVRVPGNGTIA
                     APSAGPTPPAASAPSALAPLASP"
     gene            1974..2216
                     /locus_tag="VGD55_00785"
     CDS             1974..2216
                     /locus_tag="VGD55_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0868910.1"
                     /translation="MEKRFLLLADVAEVLNISAAQAYALVRSGELPAIKIGGRGQWRV
                     ETPVLERYIQDAYARTRTFVSTHPFGAGDPDEASGE"
     gene            complement(2248..2802)
                     /locus_tag="VGD55_00790"
     CDS             complement(2248..2802)
                     /locus_tag="VGD55_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868911.1"
                     /translation="MRWDELFDDLDAQFNEVETAELAAEVADRSRREIALLRLVDRLR
                     PVLGRAVTLRLEGAGNVDGQLAAMGPDWLLLAEVGGREALVASSAVLSIGGLAAQTAA
                     ADSEGAVAARLNLAYALRGIVRDRSAVALTLVDGSVAAGTLDRVGADFVELAEHPAGE
                     ARRRDVVRHVRAYPLMSLAVVRRA"
     gene            2960..3775
                     /locus_tag="VGD55_00795"
     CDS             2960..3775
                     /locus_tag="VGD55_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868912.1"
                     /translation="MARTWFGSVARACVVMAATTSGLYWAAGSPADGWRVLMSSAAGA
                     SGEVGFDHLLVSVAALAAWVALGWFCTITGLAFASAAPGVVGRACTKASQRLTPRLLR
                     RLMEAVAGISVLAGPLSAAPAFAASPTPIAAASATPREASGGSSGGWTSDLDRPASQI
                     PLAGRLPVHLDRPAGPSSPYVAPSPRPTTVLAPSGSAALIAGSPHRDVHDGAGSYVVR
                     RGDALWDIAARHLGPSATAAEIAREWPRWYHANRAVIGDNAALIRPGELLVPP"
BASE COUNT          702 a         1362 c         1329 g          603 t
ORIGIN      
        1 ctgcgccaac tccgcgagcc cgcgaatcaa ccgctgaagt ccgacgggat cggccgtgcc
       61 gaccgcgacg acatggtcgg catcggccaa ggtggccagc gtcgaaccgt tgcgccgcgg
      121 cgcggtcgtg tcgtagctga gctcctcgtc ctgctcgaga ccgaacccgc aatcgacgac
      181 cgtcagcttg cacagtcgac gcgccatggc gtagaccgca gtgagcccgg ccggccgcaa
      241 ctcgggccaa cgctcggcac gggcaatgcc agtcagcacc cgaaggttgg gcgaaacgac
      301 tcgggccaac gtcgcgagcg tgcggtcgtc gagggtgccc gcattcgcat gccggacggc
      361 ggcggcgagc ccaggcgatt cgtcgagcaa tccgagcact tgcgccacca caccgccata
      421 gcaatcggcg tcggcgagca gtgtcgaaca accaagccgg gctgcctcag tcgccaacgt
      481 gaccgcgatc gtcgtccgac ctggtgcgcc ggttggaccc cataccgcaa cgagcgctcc
      541 ggcgtctgca gcctctagat tgaacgccgc agacgacgca ggctgcgcga atagctcgct
      601 tccggacgct ggcacatcag gtagagcaga acgcggttct gcgtatccga gtgtcatcgg
      661 atcgaggccg ccagtgagaa cgccttcggc gacagcactc gcgacttcgg cggagatcac
      721 cgccgggccg gcgtcggccg cgagcacgtg ccggacgccg agttgacgca gccgacgctc
      781 ggcgtcgtcg ccttgcaccc caccgctgct gcgcgccacg agcccgatga ccgcgacgcc
      841 ggccgtggcc aacctcgcga gcgcgtcacg gtcgagccgc cgcaagtccg cggaaaggat
      901 cacggcgcgt gcggtgccag cagaagccgc ggccagcagg tcagcaaggt cgacgcatcg
      961 gcggacgacg atcacgccga agtcttgccg ctcgaaagcc gacacgagat cggcctccca
     1021 gaccgcgtcg gtgacggcgg tcaggacagg gacgttcacg gtgaggccag cggtgctagc
     1081 gccgacggcg cggatgccgc cggtggagtc ggcccagccg aaggagccgc gatggtgccg
     1141 ttgccgggca cccgcacgag atcgaccgcg ccctgttgag ctgccgcgac gacggcgtca
     1201 acgtagggca gcggtaccga caacaccacc cccgaaccgt tcgagccacc aagtgcagag
     1261 tcggtgatgg tgctcgcgat cgtcacgttg gcgaggacga gctggggtgc tgcgtgtgcg
     1321 tcgccgaccg ctttcggtgt gacgtacaga tcgacctgca ctccgtgcag gtcgttgcca
     1381 cttggcatgt gcagcttcgc aaccggaatg gtgactaacc ggaccggaac gggcgggctc
     1441 gaaggcagcg cagcgagggg gatgagttgg ccatcaccaa gcggctgggt ggtcgtgcga
     1501 ccgacggggc tcggtccgtc ggcggagatg tacacaccgc cgtggtcctt gaaacgcacg
     1561 ttgacccgca gcagatcggc cttgaccagc gtggtgcccg gcgcaaggtc gcgcgatgcg
     1621 gcccagatcg cgtcgtaatg gtcagaagac gcgatgatct tcgcccccac tagcacagac
     1681 agcagtacca gcacgacgcc gacgatgagt cggccatcga gccacgaggg cgatttcagg
     1741 cgaacggctg gcacagcggg cagcgcgggc aggtcggaca cgattccccc tctgacactt
     1801 caccacggag cggcagcgac tccctcgcgg caccctcagt ctgcccgctc gcgaagcatt
     1861 gcgacgcgaa tcgacgtgtt ctgtggacaa cgcaggcaaa cgagcgcact gagtggcaga
     1921 ctcacggggt cccgtgtcgt cacagtccga gcttcctgat gggcggagag ctgatggaga
     1981 agcggttcct gcttctcgcc gatgtcgcgg aggttctcaa catctccgcc gcgcaggcgt
     2041 acgcgctggt gcgctccggt gagctcccgg cgatcaagat cggcgggcgc gggcaatggc
     2101 gggtcgagac accagttctc gagcggtaca tccaggatgc ttacgcgcgc acccgcacct
     2161 tcgtcagtac gcacccattc ggggcgggcg accccgatga ggcatcgggt gagtagcacg
     2221 ccagcccctc ggcgtcccgc ttgctgttca tgcccttcgc acgaccgcga gcgacatcag
     2281 cgggtaagcc cgcacgtgcc ggacgacatc gcgtcgcctt gcctctcctg ccggatgttc
     2341 ggctagctcg acaaaatccg cgcccacacg gtcgagcgtg cccgccgcga cagagccgtc
     2401 gacgagtgtc agcgcaaccg ccgaccgatc gcggacgatg ccgcgcagcg cgtacgcgag
     2461 gttgagccgg gcagccacgg ctccctctga gtccgcagct gcagtctgcg cggcaagtcc
     2521 accgatcgac agcacggccg acgacgcgac gagcgcctcg cgaccaccga cttcggccag
     2581 cagcaaccag tcaggaccca tcgctgcgag ttgaccgtca acgttgccgg cgccctcgag
     2641 ccgcagcgtg accgcccgac cgagcaccgg ccgcaatcga tcgacgagtc gcagcaaggc
     2701 gatttctcgc cggcttcgat cggcgacctc ggccgccagc tcagcggtct cgacctcgtt
     2761 gaactgggcg tcgagatcgt cgaaaagctc atcccagcgc acaagctcac cccactcggt
     2821 cgatttccat cattgagagc taaccacccc acaactggtc gcgtggtcgc agcgcgatga
     2881 tctcgggttg cattgacagc actctaacaa cactgtacga aggtaaacac aagcaaacgt
     2941 acgtattcgg aggctgggga tggcacgcac ttggttcggc agcgtcgcac gagcgtgcgt
     3001 tgtcatggcc gcgacaacgt ccggtctcta ctgggcggcc ggatcgccgg ccgacggctg
     3061 gcgggttttg atgtcgagtg ccgcaggtgc ttcgggcgag gtgggtttcg atcacctgct
     3121 tgtcagcgtc gcggcgctag cggcgtgggt cgcgctcggt tggttctgca cgatcaccgg
     3181 gttggcattc gcaagcgcgg cgccgggggt agtagggcgc gcgtgcacga aggcttctca
     3241 gcgactgact ccacgactgc ttcgccgact catggaggcg gtagcaggca tcagcgtgct
     3301 ggcgggtccg ctcagcgcgg ctcccgcgtt cgcagcttcg ccgactccga tcgcggcagc
     3361 ttcggcgacc ccgagggaag cgagtggcgg atcgtcgggc ggctggacat ccgaccttga
     3421 ccgaccggcg tcccagatac cgcttgctgg tcgcctccct gtccatcttg atcgacccgc
     3481 tgggccctcc agtccgtacg tcgcaccatc gccacgaccg acgacggtgc tcgcaccgtc
     3541 cggctcggct gccttgatcg ccgggtcccc ccaccgtgac gttcacgacg gcgcgggtag
     3601 ctacgtggtg cggcgtggag acgcgctgtg ggacatcgcc gcgcgacacc ttggaccgag
     3661 cgctactgcc gccgagatcg cccgcgaatg gccgcggtgg taccacgcga atcgggccgt
     3721 gatcggcgac aacgcagcac tgatccgtcc gggcgagctg ctcgtgccgc cgtgatgtgc
     3781 gcgtgttcta cctgcccaac tttgtggcct gactcgagcg caaggacatc atgacaagca
     3841 cagccgacac tgcggcacgc caagaaccct caagggtccg ccgtgtgcag cgcgaagcaa
     3901 acgtgctgcg gcggttgccg acgtgcgatc cgcaaccgcc gttcgacgat gagttcgatc
     3961 accggcaacc acgcgccggc aacgacgggc aacgcg
//
LOCUS       DASWVF010000046         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_383504, whole genome shotgun sequence.
ACCESSION   DASWVF010000046 DASWVF010000000
VERSION     DASWVF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383504"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VGD55_00800"
     CDS             <1..923
                     /locus_tag="VGD55_00800"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY0868913.1"
                     /translation="LERGRGWRRFLAITAPSVPVTIEAGGQTTVIESAYGGYIDAVLE
                     TDLTPGWTQVRLTCAGGAPAEVPVRVVAADERLGVITDIDDTIIVTTLPRPLVAFWNT
                     FVKREFARRPVPGIAEMLKAAVSGHPQALVVYVSTGAWNVAPAIDAFLVRHGLPRGPM
                     LLTDWGPAPDRWFRSGTEHKRAQLNRLMHDLPQLQWLLVGDDGQHDPQLYTELASNYP
                     GRIRAVAIRELTAAEQLLSHGTPEPIDEQSEPPKRSKAPVEVRGADGHALLAALRDRH
                     VIRGQLREPSPNFGRPGIAGNPTQPNTLKT"
     gene            complement(1560..>3016)
                     /locus_tag="VGD55_00805"
     CDS             complement(1560..>3016)
                     /locus_tag="VGD55_00805"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0868914.1"
                     /translation="LAVGGDQPHNIGEVLAEQPLPVAPATLSVVATSIPTPRQAFSGL
                     GRAVINTSITLAAMLALTFPSQLFNRTFEENYDEILAITRRRFGRPGARTAAEEATRS
                     KRHTIVVFSVVVAAGSLIGSLRDPGFGANTRSVLTFIATGLALLVAAATAYLVGRTYR
                     ARVHAGISAQLRALPAGLLVAALCVAVSRIAHFQPGYLYGVVCGAVFAGSLKRRELGQ
                     LTMVSSLAMLVISVAAWFIWVPVNHAATHHNAFVGVVLLDDVLASIFVAGITGTVISL
                     LPLRFMPGQALYSWHRGVWAATFGVATFGLVEILLRPSTQAVTHPGNIAWATAAVLFI
                     IFGIGSLAFREYFARHVPTDQQRIGLGAAVITALLADGDRSENELAQRLSVEASHLHA
                     TVDRLERAGWLRREKVPHAPEADAPLHLSAAANALRKSQRIGRFTRQRLILALTRTER
                     TALADLLQPSVRPDPHAGSQITAAESDRSPTKES"
BASE COUNT          541 a          955 c         1030 g          490 t
ORIGIN      
        1 accttgaacg cgggcgaggc tggcgcagat ttctggccat cactgcaccg tcagtgccgg
       61 tcacgatcga ggctggcggt cagacgacgg tgatcgagtc ggcatacggc ggctacatcg
      121 acgcggttct cgagacggac ctaaccccgg gatggacgca ggtgaggctg acgtgcgcgg
      181 gaggtgcacc ggcggaggtt ccggttcggg tggtggccgc cgacgaacgc ctcggtgtca
      241 tcaccgacat cgacgacacg atcatcgtga ccacgctgcc acgcccgttg gtagcgtttt
      301 ggaacacctt cgtcaaacgt gagttcgccc gccggccggt acccgggatc gccgaaatgc
      361 tcaaggccgc ggtcagcggc catccgcaag ccctcgtggt gtacgtgtcc actggtgcgt
      421 ggaacgtcgc ccccgccatc gacgcgtttc tggtgcggca cgggttgcct cgcgggccga
      481 tgttgctcac cgattggggc cccgccccag accggtggtt ccgatccggg acagaacaca
      541 agcgggccca actgaaccgg ctcatgcacg accttccgca gcttcaatgg ctgctcgtcg
      601 gcgatgacgg ccaacacgac ccgcagctct acaccgaact cgccagcaac taccccggtc
      661 ggatacgcgc agttgcgatc cgcgagctca ccgccgccga gcaactgctc agccacggca
      721 cccccgaacc gatcgacgag cagtccgaac cacccaaacg atcaaaggcc ccggttgagg
      781 ttcgtggcgc tgacggtcac gcactgctgg cagcgctccg cgaccggcac gtgatccggg
      841 gacagcttcg cgaaccgtcg cccaacttcg ggcggcccgg cattgcagga aatcccacgc
      901 aaccgaacac acttaagacc tgatgccgcg acgcgcaggc agcgggcgac cacgcagcca
      961 ccgcaaatcc aggatggttg cgacggtggt cgtgacgaaa tccgggtcgg ttctcacgtc
     1021 acccgtgcat gaagtgtccg gctgcaacgg cgggtggatg agccgattgg agatagcggt
     1081 ggcgcccgtg acgtggcggc gtgtggtcgc gtcgtatccg ctcgggtttg tcacggcgtc
     1141 gccggaggag gccgctgcca tcgccacctg agccaagacc gcatcggtgc gagaaaccac
     1201 tcgacgtcgc gccgcaaacg cccacgccac aagtgggcct tacctcaagc ggcggcggct
     1261 gctgccagag accggcggat catttccaag cgggagcaga gtgtggcgtc cgcggcgtga
     1321 atgcgagtcc ggcgtgtccg gcctgcgggt tggggtccac tttgtgtcgc cgcgaattcc
     1381 cggcagaggc cacggagtcg tttgcggcgc aaagtgcgcc gcgtttcatc gccgagctca
     1441 ccggtctttc gtcgtcatcg cccatatcca tgtcgacacc ttgagcagtc cgtatcggcg
     1501 gcgaccttga gacctgaccg agtcttgacg ggcctctgaa acagagccag cgctcggcct
     1561 tacgactcct tggtgggcga tcgatcagat tcggctgcgg tgatctgcga tccagcgtga
     1621 ggatcaggcc taactgaggg ctggagcaga tcggctaatg cggtgcgctc ggttcgggtt
     1681 agggcgagga tgagtcgctg ccgggtgaac cgaccgatgc gttgcgactt tcgcagggcg
     1741 ttggccgctg cgctgaggtg caacggcgca tcggcttccg gggcgtgtgg caccttttcg
     1801 cgacgaagcc agcccgcgcg ttcgaggcgg tcaacggttg catgtagatg gctggcctcg
     1861 acactgagcc gttgcgcgag ttcgttctct gatcggtcac cgtcggccag cagggccgtg
     1921 atgacagcag cgccgagccc gatgcgttgc tggtcggttg ggacgtggcg cgcgaagtat
     1981 tcgcggaacg ccaacgagcc gatgccaaag atgatgaaca gcaccgccgc ggttgcccac
     2041 gcgatgttgc cggggtgagt gacagcctga gtcgagggcc gcagcaggat ttcgacgagt
     2101 ccgaacgtcg cgaccccgaa tgtcgcagcc caaaccccac ggtgccagga gtacaacgcc
     2161 tggcctggca tgaaacgcag cggcagtagg gagatcacgg tgccggtgat gccggccacg
     2221 aatatcgagg cgagcacgtc gtcgagcagg accacgccga caaaggcgtt gtggtgggtc
     2281 gctgcgtggt tgacgggcac ccagatgaac cacgcggcca ccgagatcac caacatggcg
     2341 aggctggaga ccatcgtcag ctggccgagt tcgcgccgct tcagcgaccc cgcgaagact
     2401 gcgccgcaca cgacgccgta gaggtagccc ggttggaagt gcgcgatgcg cgacacggct
     2461 acgcacaggg cggcgacgag cagcccagcc ggcagtgcac gtagctgcgc ggagatcccc
     2521 gcgtgtacgc gggcccgata ggtccgcccg accaggtagg cggttgccgc tgccaccagc
     2581 agcgctagcc cggtcgctat gaacgtcagc acggagcgtg tgtttgcgcc gaacccgggg
     2641 tcgcgcaggc tgccgatcag cgaaccggcc gcgacgacca cactgaacac gacgatggtg
     2701 tggcgctttg aacgcgtggc ttcctcggct gccgtgcgtg cgcccggtcg accgaacctg
     2761 cgtctggtga tcgcaaggat ctcgtcgtag ttctcttcga aggtgcggtt gaaaagttgc
     2821 gaaggaaacg tcagtgccag catcgcggca agggtgatcg aggtgttgat caccgcgcga
     2881 ccgagcccgc tgaaggcctg gcgcggtgtg ggaatggagg tggccacgac ggacagggtc
     2941 gcgggggcga ccggcagtgg ttgttcagcg agtacctcgc cgatgttgtg cggctgatca
     3001 cccccaaccg ccaggc
//
LOCUS       DASWVF010000047         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_408918, whole genome shotgun sequence.
ACCESSION   DASWVF010000047 DASWVF010000000
VERSION     DASWVF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408918"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..680
                     /locus_tag="VGD55_00810"
     CDS             522..680
                     /locus_tag="VGD55_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEY0868915.1"
                     /translation="MAEKYSGEAYCVKCKAKREFEGEVVVNDKGTKMAKGKCHVCGTT
                     LNRILGKA"
     gene            complement(827..1597)
                     /locus_tag="VGD55_00815"
     CDS             complement(827..1597)
                     /locus_tag="VGD55_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0868916.1"
                     /translation="MDSVFRALADPTRRGLLDELFREDGQTLGALEARFSMTRFGVMK
                     HLKLLEDAGLVVTRRHGREKLHFLNPVPIRLVHDRWVSKYAQEWTDALSDLKKGLESS
                     MEKVFEIYIRTTPERLWEAITDTEIRSKYQFGMRATSDWTPGSRYEMSTTGGALIGEG
                     VNLEIDPPRRLVQSMVALWGDDVKSEGTSRVTWEIEPVGDSCRLTVTHDQLSEGANAQ
                     LYGGWPMILSGLKTWLETGQLLTTPGSLLYATPEATRS"
     gene            1812..>2163
                     /locus_tag="VGD55_00820"
     CDS             1812..>2163
                     /locus_tag="VGD55_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868917.1"
                     /translation="MRPVVKPALRRLWRDSTTLQIGVNPASAVVVGGISEQTARLVAA
                     FDGAHDAPALRLRARALGLDESVVDRLVSMLGDASVLDDAAIDAAPLAALSRIERDRL
                     APDLASMSLLSGRLD"
BASE COUNT          388 a          722 c          661 g          392 t
ORIGIN      
        1 cgacctctcg tccacagact gtcccgccgc gtccacagca acactcccga cggtccccac
       61 ctggcccaca accttttcca cagggggtcg tgacacggcg ttgacgcgca tccgacggac
      121 tcctaacgtc ggctcgtcga agcccctggt cgggtgacgt tcgcaagggg aaggcggacg
      181 gcaacgcgac cgggggcttc acattgctca cgatgtcccg gcgcatcgtc cccggacaac
      241 cccccagcta cggctgtgga taaagttggg gacaagtctg tggacaactt gacggtgggc
      301 gcgtcgggcg cgcgtgccgc acgcgtaagc gtctctgacg tacgctggtg atcagtttcg
      361 ttcacccgca aggggggacg acgcgatcga ggcagtccga gattcgcgta ggcgatgaac
      421 ccacggaacc aactgttcgg cgttcgacca gtctgagcag tttgggactg acctgttcca
      481 atgaccccgt tcgacccccg accgtgacca aggaggaccg cgtggccgag aagtacagtg
      541 gcgaggccta ctgcgtgaaa tgtaaggcga agcgcgagtt cgagggcgag gtagtcgtca
      601 acgacaaggg caccaagatg gccaagggca agtgccacgt ctgcggcacg acgctcaacc
      661 ggatcctcgg caaggcctag ctcgccgact cgaaccacag ctgacaccaa cgaaccacag
      721 ctgacaccaa cgaaccacac gagcggaggt cgcgggaaac cgcgacctcc gctcttttgc
      781 gttctattca ggcgtattca aggcttgttc acgccacgta ggtgcgctag ctacgcgtgg
      841 cctcgggtgt ggcgtacaac agcgagccgg gggtcgtgag taactgacct gtctccagcc
      901 acgtcttcag ccccgacaag atcatcggcc agccgccgta gagctgagcg tttgcgccct
      961 cactcagctg atcgtgcgtg accgtcagcc ggcatgagtc accgacgggc tcgatctccc
     1021 aggtaacccg cgaggtgccc tcgcttttca cgtcgtcgcc ccacaaggcg accatgctct
     1081 ggacgagtcg gcgtggcggg tcgatctcga gattgacgcc ttcaccgatc aaggcacctc
     1141 cggtggtgct catctcgtag cgcgagcccg gggtccaatc ggacgtcgct cgcatcccga
     1201 actggtactt gctccggatc tcggtgtcgg tgatggcctc ccagaggcgc tccggcgtcg
     1261 tccggatgta gatctcgaag actttctcca tggaactctc cagtcctttc ttgaggtcgc
     1321 tgagcgcgtc ggtccactcc tgcgcgtact tgctcaccca gcggtcgtgg acgagccgga
     1381 tcgggactgg gttcaggaag tggagcttct cccggccatg ccgtcgcgtg acgacaagcc
     1441 ccgcatcttc gagcagcttg agatgcttca tgacgccgaa cctcgtcatc gagaagcgcg
     1501 cctctagcgc cccaagcgtc tggccgtctt cgcgaaacag ctcgtcgagc aagccccgcc
     1561 gcgtgggatc cgcgagcgcc ctgaacaccg agtccatgcg gacgacatta tgtgactgat
     1621 tagtcacatg tcaaggccga ccatggctct ggatcgccat cggttttccc acaggccccc
     1681 gctccagtcg gcgcatttct gtggacggcg gcatcgaccg tgcgctcgcc gatgccaccc
     1741 tgcggaagca gccgctcgaa cagtgtgtgc ggggatcggt tcttagagac gacacaagag
     1801 ggggtcggcc gatgcgtccc gtcgtaaagc ccgcactccg acgcctctgg cgcgactcca
     1861 cgacgctgca aatcggcgtc aaccctgcaa gcgccgtcgt cgtcggcgga atcagcgagc
     1921 agaccgcccg cctggtggct gcgttcgacg gcgctcacga tgcacccgcg ttgcgtctcc
     1981 gtgctcgcgc actcggcctc gatgagtcag tcgtcgatcg cctcgtatcg atgctgggcg
     2041 atgcgtcagt gctcgacgat gccgcgatcg acgctgcgcc ccttgccgcc ttgtcgcgga
     2101 tcgaacgcga tcgactggcg cccgacctcg cctcgatgtc cctgctatca ggtcgcctcg
     2161 atg
//
LOCUS       DASWVF010000048         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_434461, whole genome shotgun sequence.
ACCESSION   DASWVF010000048 DASWVF010000000
VERSION     DASWVF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434461"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1235
                     /locus_tag="VGD55_00825"
     CDS             117..1235
                     /locus_tag="VGD55_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868918.1"
                     /translation="MIRFDSVTKTYPDGTVAVDNLSFTAPSGKITVLVGPSGCGKTTS
                     LRMINRMIEPTSGRIWIDDRDHSSVPAAELRRGIGYVIQHAGLFPHRTIVDNVATVPL
                     LLGQNKREARARALELLERVGLPASFAKRYPAQLSGGQQQRVGVARALAADPPVMLMD
                     EPFSAVDPVVREQLQNEFLRLQGEIGKTIVFVTHDIDEAIKLGDQVAVLRVGGKLAQI
                     STPAELLSNPVDDFVAGFVGRDRGYRALGFEPVSGLQLDPETTVTIGSGVATATAVAR
                     DGWVLVVDDKQSPQGWLAVHDEAVRTSEQIGPQFLHRGGTVASVNGTFRSVLDAALSS
                     PTGRGVIIDEAGRFAGTITAANVVRMLERQPSAAEVAP"
     gene            1232..1951
                     /locus_tag="VGD55_00830"
     CDS             1232..1951
                     /locus_tag="VGD55_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868919.1"
                     /translation="MIDAGSLPSSSGVASSSPWHFWAYFNAHLHQDLTWLWDHVWLSA
                     LPVAIGLAIALPLGWLASRYRWIYPPMLSISGLLYTIPSIALFVLIPPLLGLKALNPI
                     QVPIALTVYSVALLVRVVADGLGSVPEDVLQAATAMGFGPIQRLFKVELPIAVPVIAA
                     GLRVATVSNVSIVAVAGTIGLANLGQLFTTGFQLSSPTAYYPPIVLGIVLCVALALTF
                     DLVIVLTNRLLTPWRRAVQGR"
     gene            1959..2723
                     /locus_tag="VGD55_00835"
     CDS             1959..2723
                     /locus_tag="VGD55_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868920.1"
                     /translation="MIANVFKWLTDPSHWRGGNGLIGIGAQLQAHITYSFVAVLIALA
                     IALPLGLFIGHTGRLSFIVSAANTLRALPTVGVLVLLTVMISPNFHGRTDFGYLIPTE
                     IVLVLIAIPPILSNTYAGVQNVDLAVRDAASAMGMTGSQVLRQVELPCSLPLIFSGLR
                     SASLQVIATATIASYLPLGGLGRFIYDGLAQQDFPQMIGGGVLVALLALLTDSVLAFI
                     QRHVVSRGVSGRFSKKAMTASGPAAADIVEAEVAAV"
     gene            2804..>3505
                     /locus_tag="VGD55_00840"
     CDS             2804..>3505
                     /locus_tag="VGD55_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0868921.1"
                     /translation="MKRKAVMIGAVGIAIAMSVSACSSSKKSSSGGATVASKLILGGP
                     PEFQTRVDGIPGLKNNYGVVFGSYKVLDTGGPVTVAALKNGQVDAADLFTTDPSIAAN
                     NFVILSDPKSNFAAQNVLPIINKTKATAGVTKTLNAISAKLDTPGLGALMVKVITNKE
                     DPDAVAKGWLTTEGLDATGTDASGVSIKVGSANFPENVLLADIYADALKDQGATISET
                     LNIGSREKYVPALEDG"
BASE COUNT          588 a         1211 c         1064 g          642 t
ORIGIN      
        1 gacgcgctgg ccgcccaact cgctccagac cccacgcatg accgggcact cgaccgggca
       61 ctcgaccggg cacccgaccg ggcacagcac cgcgcacaag aaagggtggc gtcgcgatga
      121 ttcgcttcga ttcggtaacc aagacctacc ccgatggcac cgtcgcggtc gacaacctca
      181 gcttcaccgc gccgtccggc aagatcactg tgctcgtcgg gccctcaggc tgcggcaaga
      241 caacgagctt gcgcatgatc aaccggatga tcgagccgac cagcggccgc atctggatcg
      301 atgaccgtga ccactcgagc gttcctgccg ccgaactgcg gcgcggcatc ggctacgtga
      361 tccagcacgc cggccttttc ccgcaccgca cgatcgtcga caacgtcgcg acagtgccgt
      421 tgctgctcgg gcagaacaag cgcgaggcac gcgctcgcgc gttggagttg ctcgagcgcg
      481 tcggccttcc cgcgagcttc gcgaagcgtt accctgcgca actttccggc ggccagcagc
      541 aacgcgtcgg cgttgcccga gcactcgccg ccgatccccc ggtgatgttg atggacgagc
      601 cgttcagcgc cgtcgacccg gtggtgcgcg agcaacttca gaacgaattt cttcggctcc
      661 agggcgagat cggcaagacg atcgtgttcg tcacgcacga catcgacgag gccatcaaac
      721 tcggcgacca ggttgcggtg ttgcgcgtcg gcggcaagct cgcgcaaatc tcgacacccg
      781 ccgaactcct gtcgaacccg gtcgacgact tcgttgccgg cttcgtaggg cgcgaccgcg
      841 gctaccgcgc cctcgggttc gaaccggtct ccgggctgca actcgatccc gagacgaccg
      901 tcaccatcgg gtccggcgtc gcgacggcaa ccgctgtcgc gcgcgacggt tgggtactcg
      961 tcgtcgacga caagcagtcg ccgcaggggt ggctcgccgt ccatgacgag gccgtgcgta
     1021 cctcggagca gatcgggccg cagttcctgc accgcggcgg cactgtcgcc tcggtcaacg
     1081 gcacgtttcg ctcagtgctc gacgccgcgc tgtcgtcgcc gaccggccga ggcgtgatca
     1141 tcgacgaagc cggccggttc gccggcacga tcaccgccgc gaacgtggtg cggatgcttg
     1201 agcgccagcc aagtgccgcg gaggtggcgc catgatcgac gctggctcgc tgccctcgtc
     1261 gtccggcgtt gcctcgtcat ctccttggca tttctgggcc tacttcaacg cgcacctgca
     1321 ccaagacctg acctggctct gggatcacgt ctggctctcg gcgctgcccg tcgcgatcgg
     1381 cctcgccatc gcgttgccac tcggctggct cgcgagtcgc taccggtgga tctacccgcc
     1441 gatgctgtcg atttccgggc tgctgtacac gatcccgtcg atcgcgctgt tcgtgctgat
     1501 accgccgctc ctcggcctga aagccttgaa cccgatccag gtaccgatcg cgctcactgt
     1561 gtactccgtc gcgctgctcg ttcgcgtggt ggcagacggt cttggatccg tgcccgaaga
     1621 cgtgctgcag gcggcgaccg cgatggggtt cgggccgatt cagcggctgt tcaaggtcga
     1681 gttgcctatc gcggtcccgg ttattgctgc tggccttcgg gtggcgaccg tgtcgaacgt
     1741 cagcatcgtc gcggtcgcag gcacgatcgg cctcgccaac ctcggccagt tgttcaccac
     1801 ggggttccaa ctgtccagcc cgacggccta ctacccaccg atcgtgctcg gcatcgtcct
     1861 atgcgtcgcg ctggcgttga cgttcgacct cgtcatcgtg ttgaccaacc ggctcctcac
     1921 accgtggcga cgggcggtgc agggccgatg atcgactcat gattgccaat gtcttcaagt
     1981 ggctcaccga cccctcgcat tggcggggtg gcaacggcct gatcggcatc ggcgctcagc
     2041 tgcaagccca catcacctac tcgttcgttg cggttctgat cgcgctggca atcgccctgc
     2101 cgctcggact gttcatcggc cacaccggca ggttgtcgtt catcgtgagc gccgcgaaca
     2161 cgttgcgcgc gctgccgacg gtcggcgtcc ttgtgctgct gaccgtcatg atctcgccga
     2221 acttccacgg ccgcaccgac ttcggctact tgatcccgac tgagatcgtg ctcgtcctga
     2281 tcgcgattcc gccgatcctg tcgaacacct acgccggcgt gcagaacgtc gacctcgcgg
     2341 tccgcgatgc ggcgtcggcc atgggcatga ccggctcgca ggtactgcgc caagtcgagc
     2401 tcccttgcag tctcccgctc atcttctccg gcctgcgcag cgcctccttg caggtcatcg
     2461 cgacggcaac gatcgcgtcg tacttgccac tcggtggcct agggcggttc atctatgacg
     2521 ggctggcgca acaagacttt ccacaaatga tcggtggcgg cgttctcgtt gcgctgcttg
     2581 cactcctcac cgattcggtg ctcgcgttca ttcagcgaca cgttgtttcc cgcggtgtct
     2641 caggccgctt ttccaagaaa gccatgacgg cgagcggacc agccgctgcc gacatcgtcg
     2701 aagcggaggt cgcggccgtc taacgatccc aacgtcatcg aaaacgtcga tgttcgaccc
     2761 tcttgaacgc ccctggttcg atctgccaga cataaggagt cccatgaagc gcaaggcagt
     2821 aatgatcggt gctgtaggca tcgcgatcgc gatgagtgtg tcggcttgca gcagttccaa
     2881 gaagtcctcg tccggtggcg ccaccgtcgc aagcaagctg atcctcggcg gcccgccgga
     2941 gtttcagaca cgtgtcgacg gcatccccgg gctcaagaac aactacggcg tagtgttcgg
     3001 cagctacaag gtgctcgaca ccggtggccc ggtcaccgtc gccgccctga agaacggcca
     3061 ggtcgacgcg gctgacctct tcactaccga cccgagcatt gcggcgaaca acttcgtgat
     3121 cctgagcgac ccgaagagca actttgccgc tcagaacgtg ctcccgatca tcaacaaaac
     3181 gaaggcgacc gctggcgtca cgaagacgct gaacgccatc tcggccaagc tcgacacccc
     3241 cgggctcggc gccttgatgg tcaaggtcat cacgaacaag gaagatcccg acgccgtcgc
     3301 caagggttgg ctcaccaccg aaggtcttga cgcgaccggc accgacgctt cgggcgtctc
     3361 gatcaaggtc ggctcggcga acttcccaga gaacgtgctg ctcgccgaca tctacgccga
     3421 cgcgctgaag gaccagggag cgaccatctc ggagacgctc aacatcggca gccgcgagaa
     3481 gtatgtgccc gccctcgaag acggc
//
LOCUS       DASWVF010000049         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_26845, whole genome shotgun sequence.
ACCESSION   DASWVF010000049 DASWVF010000000
VERSION     DASWVF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26845"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGD55_00845"
     CDS             complement(<1..194)
                     /locus_tag="VGD55_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868922.1"
                     /translation="MSDDTFDGSGVADGPGETAVDATGALGGADVGAAEPPWGVGDSA
                     DERGELLAPTAGPAGCDVVS"
     gene            181..1239
                     /locus_tag="VGD55_00850"
     CDS             181..1239
                     /locus_tag="VGD55_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868923.1"
                     /translation="MSSLTLPTAYTFFGLDTDSGPLSLNGAVASTDPNAPCIRTPVDA
                     TALTLGAVTTGSCDDPELSGERVAIDVINPDGGSPAFTTSIAVTVTDPKTGTPKTGPV
                     IMRISEASNTNPVVAYGGGSMWIYDVDTSNGPEAIQVSATSGQVQDVARTPELYRPIM
                     VANTDGLWLGNSIEGGQLPGTLYHVAPGSHVVTTALSSSNNDVVDWMVADAGHVWAGT
                     RPAGGSLLSLRRFDGPRATVAFHTPEPSLEAGTNSVVGDQQDGLWLTTPDPPFGDHAS
                     PTDNQHLDVVRLDPNTGKPTVEAQLPPLDQLAAEMQTVAGQAAFYAGHYFLLQTLQSP
                     SESGYTGFTQLLRITLLP"
     gene            complement(1364..2572)
                     /gene="hemW"
                     /locus_tag="VGD55_00855"
     CDS             complement(1364..2572)
                     /gene="hemW"
                     /locus_tag="VGD55_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0868924.1"
                     /translation="MPSTLPDGDPMPADGALPPDVRAHGPFSMYVHVPYCASRCGYCD
                     FNTYTASELGGGASQASYAADAVREIAFARRVLGSLDLPVQTVFFGGGTPTLLPVADL
                     AVMLRAIDSEFGLAPSAEVTTEANPESVSARSLAELRAAGFTRISFGMQSVRPHVLAV
                     LDRVHTPGAPALRVAEAKAAGFEHVSLDLIYGTPGESDDDWRASLDAAIAAEPDHISA
                     YSLIVEDGTRLAGRVARGELPRPDDDVLADRYLIADSALAAAGFGWYEVSNWALPGGS
                     CRHNLAYWRSFDWWGIGPGAHSHVGGVRWWNVKHPTAYAARVVAGVSPGFARELLDAP
                     TRRVERIMLQLRLSEGLSIAELDEGGRVAGELEVSAGLLDAAAFARGRAVLTLRGRLL
                     ADGVVARLLG"
BASE COUNT          493 a          990 c          890 g          406 t
ORIGIN      
        1 aacgacacga cgtcgcagcc ggccggcccg gccgtcggtg caagcagctc gccgcgttcg
       61 tcggcgctgt cccccacacc ccagggcggt tcggccgcac cgacgtccgc gcctccgagc
      121 gcgccggtcg catccaccgc cgtctctccg ggcccgtctg ccacgccact gccgtcgaac
      181 gtgtcatcgc tcacgctgcc gaccgcgtac accttctttg gcctcgacac agactccggg
      241 ccgttgtcgt tgaacggtgc ggtcgcgtcg accgacccaa acgccccgtg catccgaacg
      301 cctgtcgatg cgaccgcgct gactctcggc gcagtgacga cagggagctg cgacgatccg
      361 gagctgtccg gtgaacgggt agcgatcgat gtcatcaatc ccgacggcgg ttcccccgct
      421 ttcacgacgt cgatcgccgt taccgtcacg gacccaaaga ccggcacgcc gaagacgggc
      481 ccggtcatca tgcggatcag tgaagcgtca aacaccaatc cggtggtggc ctacggcggt
      541 ggctcgatgt ggatctatga cgtcgacacg agcaacgggc cagaggcgat tcaggtctcg
      601 gcaacctcgg gtcaggttca ggatgtggcc cgcactcccg agctctatcg cccgatcatg
      661 gttgcgaaca ccgacgggct ttggctgggc aactcgatcg agggcgggca gctgccaggc
      721 acgctctacc acgtggcgcc cgggtcgcac gtagtcacga cagcgttgtc tagttccaac
      781 aacgacgtgg tcgactggat ggtcgccgac gccggtcatg tgtgggctgg cacccgaccg
      841 gccggtggaa gtctcctgag cctgcggcgt ttcgacgggc cgagggcgac cgtcgcgttc
      901 cacacacccg agccgagtct tgaagcggga actaactctg tcgtcggcga ccagcaagac
      961 ggactctggt tgacgacgcc cgacccgccg ttcggtgacc acgcatctcc gacagacaac
     1021 cagcacctcg acgtggtgcg gctcgacccg aacaccggga agccgaccgt cgaggctcag
     1081 ttgccgccac tggaccaact ggccgcagag atgcaaaccg tcgccggaca agcggcgttc
     1141 tacgccggcc actacttcct gcttcaaaca ctgcagtcac cgtcggagag cggctacacc
     1201 ggcttcaccc agttgctgcg catcaccctg ctgccgtaac gagaccgcaa ccaccccggc
     1261 aaccaccccg gcgtccgcgg caggtcggga ttgtggagtg tggggagtgc tctaggtacc
     1321 tccagactcc acgttgagcc ggacggcggg tggcgggcgt cggtcagccg agcaggcggg
     1381 cgacgacgcc gtcggccaac agccggccgc gcaacgtcag caccgcgcgc ccgcgggcga
     1441 acgccgctgc gtcgagcaac ccggccgaca cctcgagctc accggcaacg cgcccgccct
     1501 catcgagctc ggcgatcgaa aggccctcgg agagtcgtag ctgcaacatg atccgctcga
     1561 cccgccgcgt cggggcatcc agaagttccc gcgcaaaacc cggactcacg ccggcgacaa
     1621 cccgagcggc gtaggcggtc ggatgcttca cgttccacca gcggactccg ccaacgtgac
     1681 tgtgcgcgcc ggggccgatc ccccaccagt cgaaactgcg ccagtacgcc aggttgtgcc
     1741 gacacgagcc gccaggcagc gcccagttcg acacctcata ccaaccgaac cccgcggcgg
     1801 ccaacgccga gtcggcgatg agataccggt cggccaacac atcgtcgtcc ggtcgaggta
     1861 actcaccgcg cgcgacgcga cccgcaagcc gcgtgccgtc ctcgacgatc agcgagtacg
     1921 cgctgatgtg gtcgggctcg gcggcgatcg cggcgtcgag cgacgcccgc caatcgtcgt
     1981 cggattcgcc cggcgtgcca tagatgagat cgaggctcac gtgctcgaat ccggccgcct
     2041 tagcctcggc gacccgcaag gccggcgcac ctggtgtgtg cacccggtcg agcactgcga
     2101 gcacgtgcgg tcgcaccgac tgcatgccga agctgatccg cgtgaacccc gcagcccgca
     2161 actcagccaa cgagcgggcc gagaccgatt cggggttggc ctccgtcgtc acctccgcag
     2221 acggcgccag cccgaactcg gaatcgatcg cccgcaacat gacggcaagg tccgcaaccg
     2281 gcagcagcgt cggcgtcccg cccccgaaga agacggtctg cacgggcaga tcaagggagc
     2341 ccagaacccg ccgcgcgaac gcgatctcgc gcacagcatc agccgcatac gacgcctggc
     2401 tcgcgccgcc gccgagctca gaggccgtgt aggtgttgaa gtcgcagtag ccgcagcgcg
     2461 aagcgcagta cgggacgtgg acgtacatgc tgaagggccc atgagcgcgg acgtcggggg
     2521 gcagtgcgcc gtccgccggc atcgggtcgc cgtcgggcaa ggtcgagggc acacgcatat
     2581 tgtgtcgggc gcatcaattt ggtgttgccg atgtagtgcg ccgaacgcta caaaggagca
     2641 caattgagcc cgccctagga ggcagcggcc tgatgttttg gcttgtcttg tggctcagca
     2701 tcacggtggt gatctgcgtg accgccgtcc tccctgtcgc gcgcacaccc ctgcacacgc
     2761 gtgccgccga cgtgatcgt
//
LOCUS       DASWVF010000050         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_128754, whole genome shotgun sequence.
ACCESSION   DASWVF010000050 DASWVF010000000
VERSION     DASWVF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128754"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..468)
                     /locus_tag="VGD55_00860"
     CDS             complement(187..468)
                     /locus_tag="VGD55_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868925.1"
                     /translation="MTLLDKAKVAAEKAAARGKQGVQQGQAKIDAIQTKRKADVLLRE
                     LGSAYYAERRHGGSHDVVESALEALDGHEKEHGTVEPESDEPTAEPDAE"
     gene            623..925
                     /locus_tag="VGD55_00865"
     CDS             623..925
                     /locus_tag="VGD55_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868926.1"
                     /translation="MNDKKSGRHAMRRPPAARNHETAPAGNVSVIVAELNSVRCRWCT
                     DDLEHCHESLVLHAAGRAQCMDADCGVPEEAHHIVVACGDFGCGCARALPDVAESA"
     gene            complement(946..2409)
                     /locus_tag="VGD55_00870"
     CDS             complement(946..2409)
                     /locus_tag="VGD55_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY0868927.1"
                     /translation="MTLVAGVDSSTQSCKVVIRDADTGALVREGRAAHPDGTEVDPGA
                     WWTALVAALRDAGGIDDVAALSVAGQQHGMVCLDSAGDVVRPALLWNDTRSAPDAAAL
                     VSELGAGDSQAGAQAWAEAVGSVLVASFTITKLRWLARMEPQNAARTAAVCLPHDWLT
                     WRLASEGVAGTTRDLSALTTDRSDASGTGYWSPFTGEYRADLLAGAFGATPLLPRVLG
                     PTDVAGVTATGVRLGVGAGDNAGAALGLGAQRGDVVVSIGTSGTVFGVSPTPTADRSG
                     DVAGFADATGQFLPLVCTLNAARVLDATAAMLGVDAATLAELALSAPPGADGLVVVPY
                     LDGERTPNRPNATGSVHGWRLGNSTAAHLARASVEGVLCGLADGLDALVAQGFEVERI
                     VLIGGGARSAAIRGIAPAVFGRPVVVPPPGEYVADGAARQAAWMLEIAQNSGTRTELE
                     QPPSWKLGDLGEIATYEAEPAPWVRAQYAEVRDLQPI"
BASE COUNT          405 a          880 c          789 g          400 t
ORIGIN      
        1 gatcgcagcg ccacacaccg tgagagccgc acggcggcat tgatccgccg acagttggat
       61 catcgcgccg acgtcgtgat gtgactgctc catgagcgcc tttctctcgt gatgccggtc
      121 aaagacctgc ccctgctgca cgctcttcta acgcgcgacc acgcgtgagc tatcccgacg
      181 ccggcactat tcggcgtccg gctctgctgt tggctcatcg ctttcgggct cgacggtgcc
      241 gtgctctttt tcatggccgt cgagtgcctc gagcgccgac tcgacaacat cgtggctgcc
      301 gccgtggcgc cgctcggcgt agtaggccga tccgagctca cgcaacagga cgtcggcctt
      361 ccgcttcgtc tggatcgcgt cgatcttcgc ttgaccttgc tgcacgcctt gcttcccgcg
      421 cgcagctgcc ttctcggcag cgacctttgc cttgtccaac agcgtcatga tggatccctt
      481 cattcggagg gatcacgtta ccgccgagcg cagccacggc cgaaagggcc cgagcttgac
      541 gggcggatgg gcgtctcggg tcaaccgttt ggtcgggcgc gtcgcaacat aattcgctcg
      601 cgccgcgcct accgtcgtgg tcatgaacga caagaagtcg ggtcgccatg caatgagacg
      661 accacccgcc gcgcgcaacc atgagaccgc gcccgccggc aatgtctcgg tgatcgttgc
      721 cgagctcaac tcggtgcggt gccgctggtg caccgacgat ctcgagcact gccacgaatc
      781 tctcgtgctg catgctgccg gcagagcgca gtgcatggac gccgactgcg gcgtccccga
      841 ggaagcgcac cacatcgtcg tggcctgcgg cgacttcggc tgcggatgtg cccgcgccct
      901 acctgatgtc gcggagagcg cgtagcgagt cgggctattg cggtcttaga tcggctgcag
      961 gtcacgcact tctgcgtact gtgcgcgcac ccacggagcc ggttcggcct cgtacgtcgc
     1021 aatttcgcct aggtcaccca acttccacga cggcggctgc tcaagctcgg tgcgggtgcc
     1081 agagttctgt gcaatctcga gcatccaggc ggcctgccgc gccgcgccat cggcgacgta
     1141 ctcacccggc ggcggtacga cgaccggccg accgaacaca gcgggcgcga tgccacgaat
     1201 tgcggccgac cgcgcgccgc cgccgatcag cacgatcctt tcgacctcga acccctgagc
     1261 taccagcgcg tcgagaccgt cggcgagccc gcacagcact ccttcgacgc tggcgcgcgc
     1321 cagatgagcg gcggtcgagt tcccaaggcg ccacccgtgc acgctgcccg tggcgttcgg
     1381 ccggttcgga gttcgctcgc catcgaggta cggcacgacg acaagaccgt ctgcgccagg
     1441 cggtgccgag agcgccagtt cggcgagcgt cgcggcgtcg acgccgagca tcgcggccgt
     1501 cgcgtcgagc acccgggcgg cgttgagcgt gcacacgagc ggcaagaatt gcccggtggc
     1561 gtcggcgaac ccggcgacgt caccggaacg atccgccgtc ggggtcgggc tgacaccaaa
     1621 caccgtgcct gaggtgccta tcgacacgac cacatcgccg cgttgcgcac caagaccaag
     1681 ggccgcaccg gcgttgtcgc ccgcaccgac cccaagccgg acgccggtcg cagtgacacc
     1741 cgccacatct gttggcccga gtacccgtgg caacaacggc gtcgcgccga acgcgcctgc
     1801 aagcaaatct gcgcggtact cgccggtgaa cggcgaccaa tagccggtgc cgcttgcatc
     1861 gctgcggtca gtcgtcaaag ccgacaggtc gcgggtagtc cctgcaaccc cctcgctcgc
     1921 cagccgccac gtcaaccagt cgtgcggcaa acacaccgca gcagtgcgag ctgcgttctg
     1981 cggctccatc cgggcgagcc agcgcagctt cgtgatcgtg aaggacgcca ccagtacgct
     2041 gccgaccgct tcggcccagg cttgtgcgcc agcctgcgaa tcgccggctc caagctcaga
     2101 caccagcgcc gcggcgtccg gtgctgatcg tgtgtcgttc cacagcagtg ctggcctgac
     2161 gacgtcaccg gccgaatcga gacagaccat gccgtgctgt tggccggcga ccgacagcgc
     2221 agcgacgtcg tcgatgccgc ccgcgtcgcg aagggccgct acgagagcgg tccaccaggc
     2281 accaggatca acctcggtgc cgtcggggtg tgcggcacgc ccctcgcgaa cgagcgcacc
     2341 ggtgtcggcg tcgcggatga ccaccttgca cgactgggtc gaagaatcga cgccagcgac
     2401 cagtgtcacg tcaggccgct gtcagatcag cgcgcaccga gcacgtgctc gatggccaac
     2461 tggtcgagct gggt
//
LOCUS       DASWVF010000051         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_77834, whole genome shotgun sequence.
ACCESSION   DASWVF010000051 DASWVF010000000
VERSION     DASWVF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77834"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1517
                     /locus_tag="VGD55_00875"
     CDS             336..1517
                     /locus_tag="VGD55_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0868928.1"
                     /translation="MKSVALGIGLGVIALALGGSLAFWRRGNRGLGTPLQRTTYDVLH
                     TAGSAARALRAGLTRESAARSAPALRQLLAARAMAITGAAGLLAWDGPGIDHAPSVLV
                     GARQVMGNGKAIVLGPRDLKCADGVDCPLRSGVVVPLTSSGVVVGTLVALAPSTRAGL
                     LRAAGEVARFASTQLELAELDTARARAAEAELRFLRAQISPHFVYNALTAIASFMRTD
                     PDRARDLLLGFADFIRYSFRSRGQFATIADELQAVDTYLELERARFGDRLQVVLRIAP
                     EVLAVAVPCLLIQPLVENAVRHGLENKEGVGHVTISAQDGDVECVLSVEDDGIGMDPE
                     VARQQLSGRRDHGVGLTNVDERLRQVFGADHGLTVETALGAGTKVTVRIPKYQAGVRA
                     S"
     gene            1514..2314
                     /locus_tag="VGD55_00880"
     CDS             1514..2314
                     /locus_tag="VGD55_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0868929.1"
                     /translation="MTASASNWSPQPDAAGLRVLVVDDEAPARDDLQFLLREQPEVSV
                     VDVAADATEALRLLQGEHYDGMFLDIRMPGLDGLELARLLLRFTDPPIVIFVTAYEAH
                     AVAAFQAHARGYLLKPVSSDRLADAVAQIAEAHGSRPGPPGGESARNDMPAVPVDANG
                     RTWMVERADVCWLQANGDYVRLHTVHGGAHLVRLPLGLFEERWADHGFVRIHRSYLIA
                     LRHVVEVRTGAAGAAVVRVAGQDLAVSRRHAAELKRRLLRVQHGGRQQ"
BASE COUNT          368 a          823 c          822 g          419 t
ORIGIN      
        1 gagagtcctt tgcctcgtcg tagatgtgct cgctcgtgac cgtgttcggg ccggcgaaat
       61 gggaaggcgg cgggaagctg cgcgactcgt cgaacagggc ctcgatggtc ggatcgggca
      121 tcagtcgcct cccagcagat cgctactggg cagagactgg gagcggttgc gggatttttc
      181 aacggctgtc accgcgtggc gcgccgagcg gtcttttcgc gcgccgagcg gtcttagcag
      241 tcgccaagcg gtcagccgcg tccgcagcca agtccagact gcttcgatcg ccgaggagcc
      301 tggaactgcc gccgctcgcg gcctaatgtc gtcgcgtgaa gtcggtagcg ctcggtatcg
      361 gacttggcgt gatcgctctg gcccttggcg gttcgctggc gttctggcga cgcggcaacc
      421 gtgggctcgg cacgcctctg cagcgcacga cctacgacgt tctccacacg gcagggtcgg
      481 ccgcgcgagc cctgcgcgcc ggcctcaccc gcgagagcgc ggcccgctcg gcgcccgcat
      541 taagacagct gctcgccgcc cgagcgatgg cgatcacggg ggctgcgggc ctgctcgcct
      601 gggacggccc tggcatcgac cacgcaccgt cggtgctcgt cggcgcgcgc caggtgatgg
      661 gcaacggcaa ggcgatcgtg ctcggcccgc gcgacctcaa atgtgccgac ggcgtcgatt
      721 gcccgctgcg ctcaggggtc gtcgtcccgc ttaccagcag cggtgtcgtc gtcggaaccc
      781 tcgtcgcgct cgccccgtcg acccgcgcgg gcttgttgcg cgcggcaggt gaggtggccc
      841 gcttcgcgtc gacccagctt gaactcgccg agctcgacac cgcgagagca cgggcggccg
      901 aggctgagct gcggttcctg cgcgcccaga tctccccgca cttcgtctac aacgcgctga
      961 ccgcgatcgc gtcgttcatg cgcaccgacc ctgaccgggc ccgcgacctg ttgctcggtt
     1021 ttgcggattt tatccgctat agcttcagaa gtcggggtca gttcgcgacc atcgccgacg
     1081 agttgcaggc cgtcgacacc tacctcgaac tcgagcgagc gcgcttcggc gatcggctcc
     1141 aggtcgtgct gcgaattgcc cctgaagtgc tcgcggtggc ggtaccgtgc ctgctcatcc
     1201 agccactagt ggagaacgcg gtgcggcacg gcctcgagaa caaagaaggc gtgggccacg
     1261 tgacgatttc ggcgcaggac ggcgatgtcg agtgtgtgct gagcgtcgaa gatgacggca
     1321 tcggcatgga ccctgaggtc gcccgccagc agctatcggg ccggcgcgat cacggtgtcg
     1381 gtctgaccaa cgtcgacgaa agattgcggc aggtgttcgg cgccgatcac ggcctgaccg
     1441 tcgaaaccgc gctgggcgcc ggcaccaagg tgacggtacg cattcccaaa taccaggcgg
     1501 gggttcgcgc atcgtgacgg catcagcgtc gaattggtct cctcagccgg acgccgcggg
     1561 gttgcgcgtc ctcgtcgtcg atgacgaagc acctgcgcgc gacgaccttc aattcctgct
     1621 gcgcgaacaa cccgaggtgt ccgtcgtcga tgtcgcggcc gacgcgaccg aagcacttcg
     1681 cctgcttcag ggcgagcact acgacgggat gttcctcgac atccgcatgc ccgggctcga
     1741 tggcctcgaa ctggcccggt tgctgctccg tttcaccgat ccgccgatcg tcatcttcgt
     1801 gacggcctac gaagcgcacg ccgtcgcggc tttccaagca cacgcgcgcg gatacttgct
     1861 caagcctgtg tcatccgatc gtctcgccga cgcagtcgcg cagatcgcgg aagcccacgg
     1921 ctcacgtccc gggcctcctg gcggcgagag tgcgcgcaac gacatgccag ccgtgcctgt
     1981 cgacgcgaac ggccgcactt ggatggtcga gcgagccgac gtctgctggc tccaggcgaa
     2041 cggcgactac gtccgcctgc acaccgtgca cggcggtgcg cacctggtgc gattgccgct
     2101 cgggctgttc gaggagcgtt gggccgatca cggatttgta cgcatccatc gcagctacct
     2161 gatcgccttg cgccacgtcg tcgaggttcg caccggtgcc gccggggctg ccgtggtcag
     2221 ggtcgccggt caagatctcg ctgttagccg ccggcatgcg gccgagttga agcgccgcct
     2281 gctgcgagtt cagcacggcg gtcgacagca gtgaaatatc gaggcggacg acggcggtga
     2341 ccatcccgac gtcacctgac cgcccggtgc gcacgaccgt gcgctcgacc ggagtggtcg
     2401 cgccacgacg cgagaccgat tcgcaggcat tc
//
LOCUS       DASWVF010000052         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_383621, whole genome shotgun sequence.
ACCESSION   DASWVF010000052 DASWVF010000000
VERSION     DASWVF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383621"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VGD55_00885"
     CDS             complement(<1..433)
                     /locus_tag="VGD55_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868930.1"
                     /translation="MSIETLVTGSNTMNVWYAGPQRQRFALLGQLSENDVIHNGTDLW
                     SYDSSSRTVTHETVTGQASDRIGTSIASAAPSLDPQTAAEKALAAIDPTTTVVVDRTA
                     RVAGRAAYQLLLTPKDARSLIGSVRIAVDSATSVPLRVQIFA"
     gene            complement(437..760)
                     /locus_tag="VGD55_00890"
     CDS             complement(437..760)
                     /locus_tag="VGD55_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868931.1"
                     /translation="MLSDQGHKMAGRRVSADSDAMTRLASRRLRWLLPAGIAAAIAAA
                     SLTMTVAANASAEPDLASKTAAQLLVAVENARPAGLSGTIVETAKLGLPDISGLAGAA
                     GAGGA"
     gene            797..1453
                     /locus_tag="VGD55_00895"
     CDS             797..1453
                     /locus_tag="VGD55_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0868932.1"
                     /translation="MRLLIVEDEHRMAAAMQRGLLHAGFTVDIAGDGIRGLEMATTIA
                     YDVIVLDVMLPGLSGYEVTRQLRAREVWTPILLASAKDGDYDQADGLDLGADDYPTKP
                     FSFVVLLARIRALLRRGVQPRPSVMVVAGLTLDPAARTVCKKDEPIDLTAREFAMLEY
                     LMRRHPLPVTKAELLEHVWSDATTEPNAVEVYAGYLRRKLGRDVVQTVRGAGYRLRAT
                     "
     gene            1450..2778
                     /locus_tag="VGD55_00900"
     CDS             1450..2778
                     /locus_tag="VGD55_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0868933.1"
                     /translation="MNPLTWWARRTLRLRVTATATVVLAVGLVLGTTVLSSLFFHSRV
                     KVVDQTVHAELATVTQLVTADALPAPLPSAPGGTAFAQVLDAADTVLAATTSASTVLP
                     LLPLSQFSHRLGHAFTTTASSLGTTPMRVEAQTATLHGVPVTVLVAVPFADVTSALDA
                     LRNVVVIVVPLVLLAAAAATWLAVGSALRPVDDMRAAADAVDVNASHTAPQLTEPPSG
                     DELRRLGQTLNRMLRRLHNAGEQQRTFIADAAHELRSPIASVQTQLEVALATDTQADD
                     WPVVAADVLADVQRLSRLADDLLLLARLDSTPPTYDDTVDLGKLVDSDGTSYLVEGNA
                     AALARMLDNLCSNARRYATNVEVDVGAADSAVVVTVDDDGPGIAADDRERVFERWVRL
                     DPGRSTSDGGSGLGLAIARSIARAHHGDITLERSPLGGLRVVISIPLQHA"
     gene            2848..3408
                     /locus_tag="VGD55_00905"
     CDS             2848..3408
                     /locus_tag="VGD55_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868934.1"
                     /translation="MTIAAGDAVDTEAAPSRRREEFLIIAAIVVALVTTTIVIWSHSA
                     KAFERGGALVTMTVPGNTARFISVDAFSGGGSAPLKSLRLNSVRPIIAKNTANADIQV
                     LLCRPIDDFGGAWLAANMPRFCSSMESFRPVSGTFYPKGTWWLVAKITPHQPGVVQIA
                     QFNVTFREGLRTGKQRVGTEFALTVT"
BASE COUNT          605 a         1180 c         1141 g          638 t
ORIGIN      
        1 cggcgaagat ctgcacccgc aacggcacgg acgtcgcgga gtcaacggcg atccgcacgc
       61 tgccgatcag cgagcgggcg tccttgggcg tgagcagcag ttggtaggcg gcacgaccgg
      121 cgacacgtgc agtgcggtcg acaaccactg tggtggtcgg gtcgatggcc gcgagcgcct
      181 tctcggccgc ggtttgcgga tcgagggatg gcgcggcgct ggcgatcgag gtaccgatcc
      241 ggtcggacgc ctgaccggtc acggtctcgt gcgtgacggt tcggctgctg gagtcgtagc
      301 tccacaggtc ggtgccgtta tgaatcacgt cattctccga cagctgcccg agcaacgcga
      361 aacgctgacg ttgcgggccg gcgtaccaga cgttcatcgt gttcgaaccc gtcaccagcg
      421 tctcgatcga caagccctag gcacccccgg cgccggcggc acccgcaagt ccagaaatgt
      481 caggcaggcc gagcttcgcg gtctcgacga tcgtgcccga gagcccggcc ggacgtgcat
      541 tctcgacggc gacgagcagt tgggcagcgg tcttcgaagc caaatccggt tcggccgaag
      601 cgttcgcagc tacggtcata gtgagcgagg cggcagcgat ggccgcggcg ataccagcgg
      661 gcagcagcca ccgcaggcgg cgggaagcaa gtcgtgtcat ggcatcacta tcggcgctca
      721 cccgccgtcc cgccatcttg tggccctgat cgctcagcat cgtcacagcc tcggcagctg
      781 tgacatcgtg acggctatgc gattgctgat cgtcgaggac gagcaccgca tggctgcggc
      841 gatgcagcgc gggttgcttc atgcaggctt caccgtcgat attgccggcg acggtatacg
      901 cggactcgag atggcgacga cgatcgcgta cgacgtgatc gtgctcgacg tgatgctgcc
      961 gggactgtcc gggtatgagg tgacgcggca actaagagca cgagaagtat ggacgccgat
     1021 cctgctcgcg tcggcgaaag acggcgatta cgaccaggcc gatgggctcg atctcggtgc
     1081 tgacgactac cccacgaaac cgttctcctt tgtcgtgttg ctcgcgcgca tccgcgcgct
     1141 gctgcgtcgt ggtgtgcagc ctcgaccatc ggtcatggtt gtcgcgggcc tgaccctcga
     1201 ccccgccgca cgaaccgtat gcaagaagga cgaacctatc gacctgaccg cgcgcgagtt
     1261 cgcgatgctc gagtacctaa tgcggcggca cccgcttcct gttaccaagg ccgaactgct
     1321 tgagcacgta tggagcgacg cgacgaccga gccgaatgca gtcgaggtgt atgccggcta
     1381 cctgcgccgc aaacttggcc gcgacgtcgt gcagacggtc cgaggtgccg gctatcggct
     1441 gcgcgcgacg tgaacccact gacgtggtgg gctcgtcgta cgctgcgact gcgcgtcaca
     1501 gcgacggcca ccgtcgtgtt ggctgtcgga ctcgttctcg gcacgaccgt tctcagctcg
     1561 ttgttctttc acagcagggt aaaagtggtc gaccagaccg tgcatgccga gctcgctacc
     1621 gtcacgcagt tggtcaccgc tgacgcattg ccggccccac tgccttcggc gcctggtggc
     1681 accgcgtttg cgcaggtcct cgacgccgct gacacggtgc tcgccgcgac aacctcggcg
     1741 agcacggtct tgccactctt gcccctgtca cagttcagcc accggctggg tcacgcattc
     1801 acgacgaccg caagctccct cggtaccaca ccgatgcggg tggaggcaca aacggcaacg
     1861 ctgcacgggg tccctgtcac tgtcctcgtc gccgtcccgt tcgctgatgt cacatcagcg
     1921 ctcgatgcat tgcgcaatgt tgtcgtgatc gtcgtcccgc tggtactgct cgccgctgcc
     1981 gcggcgacct ggcttgccgt cgggtcggca ctgcgccccg tcgacgacat gcgcgcagca
     2041 gccgacgctg tcgacgtgaa cgcgagccac actgcaccgc aactcaccga gccaccaagt
     2101 ggcgacgagc tgcgacgcct tggacaaacc ctcaatcgga tgctccgacg cctgcataat
     2161 gccggggagc agcagcgcac gttcatcgct gacgccgcgc acgagttgcg ttcgccgatc
     2221 gcgtccgtgc aaacccagct cgaggtcgcg ctggcaaccg acacccaggc tgatgattgg
     2281 cccgtggtgg ccgctgacgt tctcgccgat gtgcagcggc tgagccggct ggcggatgat
     2341 ctgttgctgc tcgccagact cgacagcacc cctccgacgt acgacgacac cgtcgatctc
     2401 gggaagctcg tcgactccga cggcacgtcc tacctcgtcg agggaaacgc ggcggccctg
     2461 gcccgcatgc tcgacaacct gtgctcgaat gctcgccgtt acgcgacgaa cgtcgaggtt
     2521 gacgtgggcg cggctgactc cgcggtcgtg gttaccgtcg acgacgacgg tcccggcatc
     2581 gcggccgacg atcgcgaacg ggtcttcgag cggtgggtgc ggcttgaccc ggggcgcagc
     2641 acgagtgatg gcggctcggg gctcggattg gcgatcgccc ggtctattgc ccgtgcgcat
     2701 catggcgaca tcacacttga gcgcagcccg cttggtggct tgcgagtggt catttcgatc
     2761 ccgctccagc acgcatagcg cgcccgccag gccacacggg ttgcccgttc ggtaaggctg
     2821 gaacgcgccg cgtgcgatca ttcgcaggtg actattgccg ccggggacgc cgtcgacacg
     2881 gaggcagcgc cgagccgacg ccgcgaggaa ttcctcatca tcgcggcgat tgtcgtcgcg
     2941 cttgtcacga cgacaatcgt catctggtca cactcggcca aggccttcga gcggggcggt
     3001 gccctggtaa ccatgaccgt gcctgggaac acggctcgct tcattagtgt cgacgcgttc
     3061 tccggtggcg gaagtgcgcc tctgaaatca ctgcgcctca acagcgtgcg accgatcatc
     3121 gccaagaaca ccgcaaacgc cgacattcaa gtgttgttgt gccgaccgat cgatgacttc
     3181 ggaggagcct ggctggcggc caacatgccg cgcttctgct cgtccatgga gagcttccgg
     3241 ccggtgtcag gcaccttcta tcccaaagga acgtggtggc tggtcgcgaa aatcacgcca
     3301 caccagccgg gcgtcgtcca gatcgcgcag ttcaacgtca cgtttcgcga aggattgcgc
     3361 accggaaagc agcgcgtcgg caccgagttc gcgctgacgg ttacctagcg cctacctgct
     3421 ctacctaact cctacccggc tgcctgctcc gagagatgtt gttgcagggc tgtcagcacg
     3481 ggcagccaga catcctcgga tccttcttcg tcgtccccgc attcgatttc gtcgacatac
     3541 agcctgaccg tgacaccgcc aggg
//
LOCUS       DASWVF010000053         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_434561, whole genome shotgun sequence.
ACCESSION   DASWVF010000053 DASWVF010000000
VERSION     DASWVF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434561"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="VGD55_00910"
     CDS             complement(<1..2229)
                     /locus_tag="VGD55_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0868935.1"
                     /translation="MSGARGTLRIYLGAAPGVGKTFAMLGEGHRRADRGTDVVVGFVE
                     THGRTHTAEAIGDLEVIPRRVINYRGSDFTEMDVDAILARHPKVALVDELAHTNVPAS
                     RNEKRWQDVEDLLAAGITVLSTVNIQHLESLNDVVEKITGVPQRETIPDAIVRSAEQV
                     DVVDLSPEALRRRMAHGNIYGPDKVDAALGHYFRIGNLTALRELALLWVADKVDDALQ
                     HYRHEHQIAETWETRERVLVAITGGPEGETLIRRAARIATRSGADLLALHVAPSDGLT
                     GANPAMLAAQRLLVESLGGTYHQVVGDDISATLIDFAHAENVTQLVLGASRRSRLGGL
                     LNGGVGIGSTTTRMSGDLDVHTVMHDQAGRRHTGPSLPRGLTTRRRIAGGLLGAVLLP
                     LLTLVLVNTRSNFNLSSDLLTYLVAVVAVALVGGLYSALATAVVASLLINYYFTPPLY
                     RWTIAEHNNIVAIAAFVIVAATVSLVVEFAARRTAQAARASAESETLATLAGSVLRGQ
                     TGVSALLDQVRETFQLASVALLERDSTGDSADAPWTVVASVGAQPCTTPAQADTEVPV
                     GERFVLTVRGRALAASDLRVLGAFAAQAVTAVEQQRLTAAAEAARPLEEADRMRTALL
                     NAVSHDLRTPLASAKAAVTGLRSADVAWTDSERAELLATADESLDRLHRLVDNLLDMS
                     RIQAGALSIRRQPVALHETVPIALDSLGPAARDIEVDIPDSLPEVETDPALLERVVAN
                     LTA"
     gene            complement(2267..>2457)
                     /locus_tag="VGD55_00915"
     CDS             complement(2267..>2457)
                     /locus_tag="VGD55_00915"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEY0868936.1"
                     /translation="DISVAYANLQAPRVARVRGISLDQVHKLIGKYTVGRTLGFMGSP
                     GVDVLQLNLALDKLTGAT"
BASE COUNT          434 a          767 c          814 g          442 t
ORIGIN      
        1 ggccgtcagg ttggcgacca ctcgttcgag caaagccgga tcggtttcga cttcagggag
       61 cgaatcggga atgtcgacct cgatgtcacg cgctgccgga ccgaggctgt cgagggcgat
      121 cggcaccgtc tcgtgcaggg ccactggctg tcgcctgatg gataacgcac cggcctgaat
      181 ccggctcatg tcgagcaggt tgtcgacgag ccggtgcaaa cggtcgagcg actcatccgc
      241 cgtagcgagt aactccgcgc gttcactatc cgtccacgct acgtcggcgc tgcgtagtcc
      301 ggtgaccgcc gctttggccg aagcaagcgg tgtgcgcaga tcatgactga cagcgttgag
      361 cagtgccgta cgcatccggt cggcctcttc gagtggtcgg gccgcctccg cagcggcggt
      421 caaccgttgc tgctcgaccg cggtcactgc ttgtgctgca aaagcaccaa gaacacgaag
      481 gtcggacgcc gcgagagcac ggccccgtac cgtcaaaaca aaacgctcac caaccggcac
      541 ctcagtgtcg gcttgcgcgg gagtggtgca cggctgcgcc ccaactgatg ccacgaccgt
      601 ccagggcgcg tctgctgagt cgccggtcga gtcccgttcg agcaatgcga cggaggctag
      661 ttggaacgtc tcgcgtactt gatcgagcag ggccgacaca ccggtctggc cgcgcagaac
      721 gctacccgcc aaggtcgcaa gcgtctctga ctctgcggat gctctcgccg cttgcgccgt
      781 gcgacgcgcc gcgaactcga ccacgagact caccgtcgcg gcgacgatga caaaggccgc
      841 gatcgccacg atgttgttgt gctcggcgat ggtccatctg tacagcggcg gagtgaagta
      901 gtagttaatg agcaagcttg caactaccgc cgttgctagt gcggagtaca ggcctccgac
      961 gagcgcgacg gcgacgacag cgacgaggta ggtcagcagg tcactcgaca ggttgaagtt
     1021 gctgcgggtg ttcacgagta ccagcgtcag cagcgggagc aggaccgcgc caagcaggcc
     1081 gccggctatg cggcgacgcg tcgtgaggcc gcgcggcaac gacgggccgg tgtggcgacg
     1141 tcccgcctgg tcatgcatca cggtgtggac gtcgagatca ccggacatgc gcgtcgtcgt
     1201 cgagccgatt ccgacgccgc cattcaacag gccgccgagc cgagagcgac gggaggcgcc
     1261 gagtacaagt tgggtgacgt tctcggcatg tgcgaagtcg atgagagtcg cagaaatgtc
     1321 gtcgccgacg acctgatggt acgtaccgcc gagtgactcg accagcaagc gttgcgcggc
     1381 gagcatcgct gggttggctc cggtaagacc gtcggacggc gcaacgtgca aggcgagcag
     1441 gtcggcgccg ctgcgggtgg caatgcgtgc agctcggcgg atcagcgtct caccctcggg
     1501 gccccccgtg atggcgacca gaacgcgttc gcgcgtttcc catgtctccg cgatctggtg
     1561 ctcgtgccgg tagtgctgca gcgcgtcgtc gactttgtcc gcgacccaca gcagtgccag
     1621 ttcgcgcagt gcagtgaggt tgccgatgcg gaagtagtgc ccgagtgctg cgtcgacttt
     1681 gtcagggccg tagatgttgc cgtgcgccat ccgacgccgc aacgcctcag gcgagaggtc
     1741 gacgacgtcc acctgttcgg cagatcggac gatcgcgtca gggatggtct ctcgttgcgg
     1801 aacaccggta attttctcca ccacgtcatt gagcgactcg agatgctgga tgttcaccgt
     1861 cgacaacacg gtgatccccg cggcgagcaa gtcctcgacg tcctgccagc gcttctcgtt
     1921 gcgtgaagct ggcacgttgg tgtgagcaag ctcgtcaacg agcgcgacct tggggtgccg
     1981 cgcgaggatc gcgtcaacgt ccatctcggt gaagtcgctg ccgcgatagt tgataacccg
     2041 gcgcgggatg acctcgagat ctccgatggc ttctgcggtg tgcgttcggc catgtgtctc
     2101 gacaaagccg acgacgacgt cagtgccgcg atcggctcgt cggtggcctt ccccgagcat
     2161 ggcgaacgtc ttgccgacgc cgggggccgc gccgaggtag atgcgcaacg ttccccgcgc
     2221 acctgacatc gacgccacct ccagactcca gaatatttcg ttcgggttag gtcgccccag
     2281 tgagcttgtc gagcgccaag ttcagttgca gcacatcgac gccagggctt cccatgaagc
     2341 ccaacgtccg gccgaccgtg tacttgccga tgagtttgtg tacttgatcg agactgatgc
     2401 cgcgcaccct ggcgactcgc ggtgcctgca ggttcgcgta tgccacggag atgtctg
//
LOCUS       DASWVF010000054         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_26963, whole genome shotgun sequence.
ACCESSION   DASWVF010000054 DASWVF010000000
VERSION     DASWVF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26963"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VGD55_00920"
     CDS             complement(<1..1028)
                     /locus_tag="VGD55_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HEY0868937.1"
                     /translation="MTRLASRRLRWLLPAGIAAAIAVASVATTAAASASGQPKLPAKT
                     AAQLLVAVENASPAGLSGTIVETAKLGLPDISGLLGSSGGSGLSLESLVTGSNTMNVW
                     YAGKDQQRFALLGQLSEDDLIHNGSDLWSYDSSTRTVTHKTLTPDESDQVGSSIASAA
                     PSLDPQAAAEKALAAIDPTTEVVVDRTARVAGRAAYQLQLSPKDTRSLIGSVQIAIDA
                     ETSVPLRLQIFAAGATSPAFQVGFTSVSFNVPNASIFKFVPPAGASIEQDTAPFGDSL
                     NDTPFGNGSNYVVRTRVSGKDLSGKGVPNGTLDTPAPPVPTNLNSKRSVTELGKGWTE
                     VIKITNAVA"
     gene            1126..>1599
                     /locus_tag="VGD55_00925"
     CDS             1126..>1599
                     /locus_tag="VGD55_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0868938.1"
                     /translation="MRLLIVEDEHRMAAAMQRGLVHAGFTVDVAGDGIRRLEMATAVG
                     YDVIVLDVMLPGLSGYEVTRQLRAREIWTPILLASAKDGDYDQADGLDLGADDYLTKP
                     FSFVVLLARIRALLRRGAVGPRPSVLVAGGLTLDPAARTVVKDDEAIDLTAREFAM"
BASE COUNT          272 a          500 c          541 g          286 t
ORIGIN      
        1 gccacggcgt tggtgatctt gatgacctca gtccagccct tacccagctc cgtgacgctt
       61 cgctttgagt tcaggtttgt cgggacgggt ggcgccgggg tgtcgagcgt gccgttgggg
      121 acgccctttc cggaaaggtc cttgccgctg acgcgggtgc ggaccacgta gttgctgccg
      181 ttgccgaatg gggtgtcgtt caggctgtcg ccaaacggcg cggtgtcctg ctcgatgctc
      241 gcaccggccg gtggcacgaa cttgaagata gacgcattgg gcacgttgaa gctgacactg
      301 gtgaacccga cctggaacgc cgggctggtc gcaccggccg cgaagatctg caagcgcagc
      361 ggcaccgagg tctccgcatc gatcgcaatc tgcacgctgc cgatcagcga ccgggtgtcc
      421 ttgggtgaaa gctgcaactg atatgcagca cggccggcaa ctctggcggt gcgatcgacg
      481 acgacctcgg tcgtcgggtc gatggccgcg agcgccttct ccgccgccgc ttgcgggtcg
      541 agcgacggcg cggcgctagc gatcgaggag ccaacctggt ccgactcgtc cggcgtcagc
      601 gtcttgtggg tgactgttcg cgtactggag tcgtagctcc acagatcgct gccgttgtga
      661 atgagatcgt cttcggacag ttgcccgagc aacgcgaacc gttgctgatc cttgcctgcg
      721 taccacacgt tcatcgtgtt cgaaccggtt accaacgact cgagcgacag gcccgagcca
      781 ccactggaac cgagcaagcc tgagatgtcc ggcaggccca gtttcgcggt ctcgacgatc
      841 gtgcccgaca ggccggcggg cgacgcgttc tcgactgcaa cgagcagctg agccgcggtc
      901 ttggccggca gcttgggctg tccggaggcg ctcgcagcag cggtagtggc gacggaagca
      961 acggcgatgg cggcggcgat accggcgggc agtagccagc ggaggcggcg cgaagcgagg
     1021 cgtgtcatgg catcactatc ggcggcaacc cgccgtcccg catctttgcg acccccggcg
     1081 ctcagcatcg tcacagcatc cggcggctgt ggcatcgtga cgcctatgcg attgctgatc
     1141 gtcgaggacg agcaccgcat ggccgccgcg atgcaacgcg ggctggtaca tgcgggcttc
     1201 accgtcgacg tcgccggcga cgggatccgc aggctcgaga tggcaacggc agtcggctac
     1261 gacgtgatcg tgctcgatgt gatgctgccg ggactctcgg gctacgaagt aacgcgacaa
     1321 ctaagagcac gagaaatatg gacgccgatc ctgctcgcgt cggcaaaaga tggcgactac
     1381 gaccaagccg acgggctcga ccttggtgcc gatgactacc tcacgaagcc attctcgttc
     1441 gtcgtcttgc tcgctcgcat tcgcgcattg ttgcgccgtg gggcggtcgg gccgcgaccg
     1501 tcggtcttgg tggccggagg actcacgctc gaccccgcag cacgaaccgt cgtcaaagac
     1561 gatgaagcga tcgacctgac cgcgcgtgag ttcgccatg
//
LOCUS       DASWVF010000055         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_307146, whole genome shotgun sequence.
ACCESSION   DASWVF010000055 DASWVF010000000
VERSION     DASWVF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307146"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VGD55_00930"
     CDS             complement(<1..1259)
                     /locus_tag="VGD55_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY0868939.1"
                     /translation="MSPARSVSASASRPQSGSGQQSRKQVQQVDRVIIRFAGDSGDGM
                     QLTGDRFTSETAAFGNDLSTLPSFPAEIRAPAGTLTGVSSFQLHFADHEILTPGDAPN
                     VLVAMNPAALKANIADVPLGADIIANADEFTLRACQRIGYSGNPLDDGTLSEYKVSAV
                     PLTSMTVEALKHIDISKKEAERAKNMFALGLLSWMYNRPTASTLAFLESKFAKNPDVA
                     KANVTAFQAGYNFGETTETFSVSYEVKPAPMKAGTYRNISGNLALSYGLIAASKATGL
                     PLFLGSYPITPASDILHELSKHKAFGVRTFQAEDEIAGVTAALGAAFGGSLGVTTTSG
                     PGVALKSEAIGLGFMLELPLIVVDVQRGGPSTGLPTKTEQADLLQAMFGRNGEAPVPI
                     VAPSTPSDCFAIALEAARIATKYRTPV"
     gene            complement(1353..2258)
                     /locus_tag="VGD55_00935"
     CDS             complement(1353..2258)
                     /locus_tag="VGD55_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868940.1"
                     /translation="MIVGLLFALGAAATTGVASVLQALAARRSHPTSGKGDAPHAPVA
                     GSVSRLVVSPLYLGGMGLDALGFVCIVTALHWLPVFLVQCASASSVGVTAIIGRRVLG
                     TVLGRRQLIALCGLGVGLILLAAGAKAEAATAVARPAQWWLVVAVAPILVAGVVTMRR
                     VGERAGGLLAALSGLAFADTGVASRVLSDARSVHDVIVAPATYALAISGIVGTAFFAA
                     ALQRTVVTTATAALFGVDTLAGSAAGLLALGDTTRHGFVVPAAVGFVITLACALLLAL
                     TESLDAPPGAAGPATPEFGAVRRGA"
     gene            complement(2272..3102)
                     /locus_tag="VGD55_00940"
     CDS             complement(2272..3102)
                     /locus_tag="VGD55_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0868941.1"
                     /translation="MKLASTTRKADKALRTVAKFGVEEVLWTRARLAVRRNARALPTP
                     ARPAPLPAVEPTAVLQDDEQWRDAVEQARRRDLPLHRDLPKNWDTLGAASTVLARVGP
                     GGRVLDAGAARYSTLLIWLYLYGLRDLVGINLEFRRPVRHGAGGCVRFEPGDATATMF
                     DAGSFDAVTCLSVIEHGVPIPEFLAEAARLVRPGGVLVVSTDYDAEPPDTTGHTAYGA
                     PVHVFSPAEIEALIKLADEAGFDLDGTFVPQHRDRPVYWKRLGLRFTFVRITFTRRAP
                     "
     gene            complement(3106..3597)
                     /locus_tag="VGD55_00945"
     CDS             complement(3106..3597)
                     /locus_tag="VGD55_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY0868942.1"
                     /translation="MADSSFDIVSKVDRQEVDNALNQSVKEIGQRYDFKDVGASIAWS
                     GETGIEIKANSEERAKAVLDVFQEKLIKRGISLKSVEAGEPKASGKEFRLAATMKEGI
                     AQDDAKKISKIIREEGPKGVQAQIQGDQLRVTSKKRDDLQEVIALLKGKDLTVALQFT
                     NYR"
     gene            3657..3738
                     /locus_tag="VGD55_00950"
     tRNA            3657..3738
                     /locus_tag="VGD55_00950"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3691..3693,aa:Tyr,seq:gta)
BASE COUNT          652 a         1250 c         1284 g          614 t
ORIGIN      
        1 aaaaccggcg tgcggtattt cgtcgcgatg cgcgcggctt caagggcgat cgcgaagcaa
       61 tccgacgggg tgctcggcgc gacgatgggc accggggcct caccgttgcg gccgaacatg
      121 gcctgcaaaa ggtcggcctg ctctgtcttc gtcggcaggc cggtcgaggg gccaccgcgc
      181 tgtacgtcaa ccacgatcaa cgggagttcc agcatgaagc cgagaccgat cgcctcgctc
      241 ttcagcgcga cgccaggacc tgaggtcgtc gtgaccccga ggctgccgcc gaacgccgcg
      301 ccgagcgccg cggtaacgcc ggcgatctcg tcttcggcct ggaacgtgcg aacgccaaat
      361 gccttgtgct tcgacaactc gtgcaggatg tcggacgccg gcgtgatcgg gtaggagccg
      421 aggaacagcg gtaacccggt tgcctttgac gccgcaatca gaccgtacga caaagcgagg
      481 ttgcccgaga tgttgcggta ggtgccggcc ttcatcggag cgggcttgac ctcgtacgac
      541 accgagaatg tctcggtggt ctcgccgaag ttgtagccgg cctggaacgc ggtgacgttg
      601 gccttcgcga cgtctggatt ctttgcgaac ttgctctcga ggaacgccag cgtcgacgcc
      661 gtcggccggt tgtacatcca cgaaagcagg ccaagcgcga acatgttctt cgcgcgttcg
      721 gcttccttct tcgagatgtc gatgtgcttg agcgcctcga cggtcatcga cgtcagcggt
      781 acggcagaca ccttgtactc agacagcgtg ccgtcgtcga gggggttgcc cgagtagccg
      841 atccgctggc aggcccgaag ggtgaactcg tcagcgttcg cgatgatgtc ggcaccgagc
      901 gggacgtcgg cgatgttcgc cttcaacgcg gccgggttca tcgcgaccag cacgttcggc
      961 gcgtcgcccg gcgtcaggat ctcgtggtcg gcgaagtgca gctggaacga cgacaccccg
     1021 gtcaacgtgc cggcaggtgc ccggatctca gccgggaagc tcggcagcgt tgacaggtcg
     1081 ttgccgaagg ctgccgtctc ggaggtgaag cgatcaccgg tgagctgcat accgtcgccg
     1141 gagtcgccgg cgaaccggat gatgaccctg tcgacttgct ggacctgctt gcgtgactgc
     1201 tggcctgatc ctgactgcgg tcgtgacgct gaagctgata cggatcgcgc tggtgacacc
     1261 gggtgtcgaa cctccttgaa gagcgccgac cccgccctgg gaacggttcg acgcgcggcg
     1321 tctatcgtac ggcgtgtcgc gtcggctagt gctcaggcgc cgcggcgcac ggccccgaac
     1381 tcaggagttg ccggtcccgc agcccccggt ggggcgtcga ggctttccgt caacgcgagg
     1441 agcagcgcgc acgcgagggt gatgacgaat ccgacggctg cagggacgac gaagccgtgc
     1501 cgggtggtgt caccgagcgc gagcaagccc gcagccgaac cggccaacgt gtcgacaccg
     1561 aacagcgcgg cggtcgcagt ggtaaccacg gtgcgctgga gtgccgccgc gaagaaggca
     1621 gtaccgacga tgcccgagat cgcgagcgcg taggtggcgg gggccacgat gacgtcgtgc
     1681 accgagcgcg catctgacag cactcgcgat gcaacgccgg tgtcggcaaa cgccagcccc
     1741 gacagcgcgg cgagcagccc gcctgcgcgc tcgcccaccc ggcgcatcgt gaccacgccg
     1801 gcgacgagga tcggcgcgac cgccaccacc aaccaccact gcgccggcct cgccaccgcc
     1861 gtcgccgcct ccgctttcgc cccggctgcg agcaggatca ggccgacgcc gagtccgcac
     1921 agggcgatca actgccgtcg gccgagcacg gtgccgagca ctcggcgtcc gataatcgct
     1981 gtgactccca cgctggacgc cgaagcgcat tgcacgagga acaccggcag ccagtgcagc
     2041 gccgtgacga tgcagacgaa cccgagagcg tcgagcccca taccgcccag gtagaggggc
     2101 gagacgacga gccggctcac cgagccggca actggtgcgt gaggggcgtc acccttcccg
     2161 ctggtgggat gtgagcggcg ggcggcgagt gcctggagca ccgatgcaac gccggtggtt
     2221 gcggccgcgc cgagagcgaa gagcagcccg acgatcacgg gcggttgatg gtcatggggc
     2281 gcggcgggtg aaggtgatgc gcacgaacgt gaagcgcaga ccgagccgtt tccagtacac
     2341 gggccgatca cgatgttgcg ggacgaacgt tccgtcgagg tcgaaccccg cctcgtcggc
     2401 gagcttgatc agcgcctcga tctcggcggg ggagaacacg tgcacgggcg cgccatacgc
     2461 cgtgtgcccg gtcgtgtcgg gcggctcggc gtcgtagtcg gtggatacaa cgagaacgcc
     2521 gcccgggcga acgaggcgcg ccgcctccgc caggaattcc ggaatcggga cgccgtgctc
     2581 gatcaccgaa aggcaggtga cggcgtcgaa ggacccggcg tcgaacatgg tcgcggtggc
     2641 gtctccgggt tcgaaccgaa cgcaaccgcc agcgccatgc cgtacgggac gccgaaactc
     2701 caggttgatg cccaccagat cgcgaagccc gtagaggtag agccagatca gcagggtcga
     2761 gtaccgcgca gcgccggcgt cgagcacccg accgccagga ccaacgcggg ccaacacggt
     2821 cgacgcggca ccgagggtgt cccagttctt cggcaagtca cgatgaaggg gaagatcgcg
     2881 tcgccgcgcc tgctcgacgg cgtcccgcca ttgctcgtcg tcttgcaaga ccgcagtcgg
     2941 ctcgaccgca ggaagcggcg caggccgggc gggcgtcgga agcgcacgtg cattgcggcg
     3001 gaccgccagc cgggcacggg tccagagcac ttcttccacg ccaaacttgg cgacggtgcg
     3061 aagcgccttg tcggccttcc gtgtcgttga ggccagcttc atgaatcagc ggtagttcgt
     3121 gaactgcagc gcgacggtga ggtccttgcc cttcagcaac gcaatgacct cctggaggtc
     3181 gtcgcgcttc ttgcttgtga cccgcaactg gtcgccctga atctgggcct gaacaccctt
     3241 ggggccttcc tcgcggatga tcttggagat cttcttggcg tcgtcctggg cgatgccttc
     3301 cttcatcgtc gcggccagcc ggaactcctt gccggacgcc tttggctcac cggcctctac
     3361 cgacttcagc gagatgccgc gcttgatcag cttctcctgg aacacgtcga gcacggcctt
     3421 cgcgcgctcc tcgctgttgg ccttgatctc gatgccggtc tcacccgacc atgcgatcga
     3481 tgcgccgacg tccttgaagt cgtaacgctg cccgatctct ttcacggact gattgagggc
     3541 gttgtcgacc tcctgcctgt cgaccttgct cacgatgtcg aaggacgaat cggccatcac
     3601 tacctcctgg gctatcctgt gctccgccgt cctgcgggac ggtatagcac cgtcacggca
     3661 ggttgcccga gtggccaaag ggagcggtct gtaaaaccgc cggctcagcc tacgatggtt
     3721 cgaacccatc acctgccaca caccacaact cgcgcggacg ccgggtggcg tccgtgtaga
     3781 gttgcggctc gcgtcacctc
//
LOCUS       DASWVF010000056         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_460125, whole genome shotgun sequence.
ACCESSION   DASWVF010000056 DASWVF010000000
VERSION     DASWVF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460125"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VGD55_00955"
     CDS             complement(<1..227)
                     /locus_tag="VGD55_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868943.1"
                     /translation="MITQSAFPVEPWTIRETRLDLGLIAQSESVFALSNGHIGLRGNL
                     DEGEPHGIPGTYLTSFYELRPLPYAESAYGNP"
     gene            complement(224..982)
                     /locus_tag="VGD55_00960"
     CDS             complement(224..982)
                     /locus_tag="VGD55_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEY0868944.1"
                     /translation="MLGLPDHVTACLFDLDGVLTDTASVHDKAWTETFDAFLKARAER
                     TGEKYVPFDPANDYAQYVDGLPRADGVRTFLRSRGIELPEGKPDDDPNAETVNGIGNR
                     KNAAVLQHIKNDGVKVFEGSRQYLKAARDAGLRRIVVSSSANTAEVLEVTGLGELIEG
                     HIDGVTIETEHLKGKPAPDTFIAGAKCAGVEPAQAAVFEDALSGVEAGRAGAFGCVVG
                     VNRLDATHGGQLRKHGADIVVNDLAELLANGKRT"
     gene            1076..1678
                     /locus_tag="VGD55_00965"
     CDS             1076..1678
                     /locus_tag="VGD55_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY0868945.1"
                     /translation="MAGIVSPGFFGRRREGAPPLPPGQYLVRDFPVLSAGPTPRVNLD
                     TWEFRIVTESGEKRTWDWAAFQALPVVDMTVDLHCVTKWSKLGTSWRGVSLDTLLEGI
                     ETAAEYALVLSYGGYTTNLPLADLLDGKAWLAFSYDGSDLTPEHGGPVRLLVPHLYLW
                     KSAKWVRGIDLRRTEQRGFWEQLGYHDYGDPWLEQRYSDD"
     gene            1648..>2344
                     /locus_tag="VGD55_00970"
     CDS             1648..>2344
                     /locus_tag="VGD55_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEY0868946.1"
                     /translation="MARAAVLGRLTWRVGTLVGFRDETPVARTLVFQIPDWPGHAAGQ
                     HVDVRLTAPDGYSTQRSYSIASAVDGETVELTVERLSDGEVSPYLSQVLSIGDQIELR
                     GPIGGWFVWDPSDDAPVLLVGGGSGVVPLMAMMRTRARVGSSALFRLLYSVRSPDSVL
                     YGVELESLRASDPSLSVEYAFTRTAPVGSARPPGRIDATFLASYGFAASLAPAVFVCG
                     PTGFVETVANLLVA"
BASE COUNT          345 a          757 c          782 g          460 t
ORIGIN      
        1 gggttcccgt acgcgctctc ggcatagggg agcggacgaa gctcgtaaaa cgaggtcagg
       61 taggtgccgg gtatcccatg cggctcacct tcgtcgagat ttccgcgcag cccgatgtgg
      121 ccgttcgaca acgcgaagac cgattccgac tgcgcgatca ggccgagatc gaggcgggtt
      181 tctcgaatcg tccacggctc gaccggaaag gctgactgcg tgatcacgtg cgcttcccgt
      241 tggcgaggag ttcggcgagg tcgttcacga cgatgtcggc gccgtgtttc cgcagttgtc
      301 ctccgtgcgt ggcgtcgagc cggttcaccc cgacgacgca tccgaacgca ccggcccggc
      361 ccgcctcgac tcctgatagc gcgtcctcga agaccgcagc ctgcgcaggc tcgacaccgg
      421 cacacttcgc cccggcgata aacgtgtcgg gcgcgggctt ccccttgagg tgctcggtct
      481 cgatcgtcac gccgtcgatg tgcccctcga tcagttcgcc aagtccggtg acctcgagta
      541 cctctgccgt gttcgcgctc gacgatacga cgatgcgccg caagccggcg tcccgcgcag
      601 ctttcaagta ttgacgcgaa ccctcgaaaa ccttcacgcc gtcgttcttg atgtgctgca
      661 acacggcggc gttcttgcgg ttcccgatgc cgttcacggt ctctgcgttc gggtcgtcgt
      721 cgggcttacc ttcgggcaat tcgatgccgc gactgcgcaa gaacgtgcgg acgccgtcgg
      781 ctcgggggag tccgtcgacg tactgcgcat agtcgttggc cggatcgaag ggcacgtact
      841 tctcaccggt gcgctcggca cgcgccttca ggaaagcgtc gaaggtctcg gtccacgcct
      901 tgtcgtgcac cgacgcggtg tcggtgagca cgccgtcgag gtcgaacagg cacgccgtga
      961 cgtggtcggg cagaccgagc atgaccgcct ccttcgctgc cgatcatcct cgccgaccta
     1021 cccacgagtc acgcgcctca cccccgcggc attagcattc ccgaaggagg tagccatggc
     1081 agggattgtg tcgccggggt tcttcggccg ccgtcgcgag ggcgccccgc cgttgccgcc
     1141 ggggcagtac ctcgtgcggg acttcccggt gctctccgct ggaccgacgc cgcgggtgaa
     1201 tctcgacacg tgggaattcc ggatcgtcac cgagtcgggt gagaagcgga cctgggattg
     1261 ggccgcattc caggcgctgc ccgtcgtcga catgacggtc gacctgcact gcgtcacgaa
     1321 gtggtcgaag ctcggcacct cgtggcgcgg cgtttctctc gacacgttgc tcgagggcat
     1381 cgagaccgcc gccgaatatg cgctcgtgtt gtcgtatggc ggctacacga cgaacctgcc
     1441 gctggccgat ctgctcgacg gcaaggcgtg gcttgccttc agctacgacg ggtcagacct
     1501 cacgcccgaa cacggtgggc ccgtgcggct gctcgtgccg catttgtacc tgtggaagtc
     1561 ggcgaaatgg gtgcgcggga ttgaccttcg ccgcaccgaa caacgggggt tctgggagca
     1621 gctcggctat cacgattacg gagacccgtg gctcgagcag cggtactcgg acgactgacc
     1681 tggcgggtcg gcacgctggt cggttttcgc gacgagaccc cggtcgcccg gacgctggtc
     1741 ttccagattc cagactggcc agggcacgct gccggccagc acgtcgacgt ccggcttact
     1801 gcacctgatg ggtattcgac gcagcgctcg tattcgatcg catccgccgt cgacggcgag
     1861 actgtcgaac tgacagtcga acgattgtcg gacggcgagg tgtcgccgta tttgtcgcag
     1921 gtgctctcga tcggcgatca gatcgagctg cgcggaccga tcggcggctg gttcgtgtgg
     1981 gatccgtccg acgatgcgcc ggtgttgctg gtgggcggcg gttcgggcgt ggtgccgctg
     2041 atggcgatga tgcgcacacg cgcccgggtt ggcagcagtg cgctgttccg gctgctgtac
     2101 tcggtgcgct cgcccgattc cgtgctgtac ggcgttgaac tggagtcgtt gcgcgcaagc
     2161 gatccttcgc tgtcggtcga gtacgcgttc acgcggaccg cgcctgtcgg ctcggcgcgt
     2221 ccacccggcc gcatcgatgc gacattcctt gcgtcatatg gctttgctgc aagccttgcg
     2281 cctgcggtgt tcgtgtgtgg cccgaccgga ttcgtcgaaa cggtcgccaa tttgctggtc
     2341 gccg
//
LOCUS       DASWVF010000057         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_27003, whole genome shotgun sequence.
ACCESSION   DASWVF010000057 DASWVF010000000
VERSION     DASWVF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27003"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..302)
                     /locus_tag="VGD55_00975"
     tRNA            complement(231..302)
                     /locus_tag="VGD55_00975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Val,seq:gac)
     gene            complement(343..414)
                     /locus_tag="VGD55_00980"
     tRNA            complement(343..414)
                     /locus_tag="VGD55_00980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Cys,seq:gca)
     gene            complement(471..543)
                     /locus_tag="VGD55_00985"
     tRNA            complement(471..543)
                     /locus_tag="VGD55_00985"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Gly,seq:gcc)
     gene            617..1537
                     /locus_tag="VGD55_00990"
     CDS             617..1537
                     /locus_tag="VGD55_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY0868947.1"
                     /translation="MSRADPVTEALPIDERWPRAGHWLSAGPGPHAIDLAVLGVPTFA
                     RSLNKAGTHTTPAAIRRALHHYTTWCASRHVDLAELWPWDMGDVDEPDRDDGGFRAQA
                     AVTTALAKARLLVTLGGDGSATVPTLQGLDLQRAGLVALDVQYDLRDGARNTSALRTL
                     FDLGLDGERVSHLGAADWAVSRSHADEAKALGMTVYPRGVVEARGVAACMSAALDSAG
                     QSGPVHVSFDLTVCDRSVVPGCLEALPGGLRAADLLSAAFIAGRDPNVRSVDIVEVDA
                     TADSADYRTVRLAALVVLEVAAGLALRPQA"
     gene            1534..>2023
                     /locus_tag="VGD55_00995"
     CDS             1534..>2023
                     /locus_tag="VGD55_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY0868948.1"
                     /translation="MTAQAFAHVDGRLASRLLEVRHDLAALDEPGFWVVVGGFEGEVT
                     CARFADVREAPLPAARWSGPKRDGWTTSLDQAAYVEGVAAIRALIAQGAVYQVNLCRV
                     LSAHSDAADLSGLATVLAAEHPSRYPALVCLPEVGIQICSASPELFLSRSRDDGVDVV
                     ATR"
BASE COUNT          309 a          676 c          672 g          366 t
ORIGIN      
        1 tttttcgtga actagacagg atcacccaac agtattcggt atccaagaca cgttggcccg
       61 cccggccgtg atgccgatgg atgcctggcg cgacagtgac acgtttatcc aaagcgattt
      121 atgtgagcta agcgccttcc gcgcttggcc ctcgcgctgg cttatggcac aagtaatggc
      181 acgatcgtga tcatcttcgg cctaaacgtg ctctgacctg cagttatgtg tgggcgttgc
      241 agggattgaa cctgcgaccc cttccgtgtc agggaagtgc tctcccgctg agctaaacgc
      301 cctacatcgt gcagcgacac cctctcacgt acagcgtcga cgaggcggag gcgggaatcg
      361 aacccgcgta cagggctttg caggcccttg cctaagccac tcggccactc cgccggcgaa
      421 ccgcggacga ccaaccgacc gaggccctgt tgacgccgtc ggcgcctccg agcggacgac
      481 gggattcgaa cccgcgaccc tcaccttggc aaggtgatgc tctaccaact gagccacgtc
      541 cgcgcgaacc agtgcagatg ccccggttgc gtccagcagc ctaaccgata ccgcggtgtg
      601 caagcatgcg ggcgtggtgt cccgcgctga tccggtaact gaagcgctgc ctatcgacga
      661 gcgttggccc cgcgccgggc actggctgag cgccggccca gggcctcatg cgatcgatct
      721 tgcagtcctc ggcgtcccga ccttcgctag gtcactcaac aaggctggca cgcacaccac
      781 cccggccgcg atccggcggg cgttgcatca ctacacgacg tggtgcgcgt cgcggcatgt
      841 cgatctcgcc gagctgtggc cgtgggacat gggcgatgtc gacgagcccg atcgcgatga
      901 cggtgggttc cgagctcagg ccgcggtgac aaccgcccta gcgaaggcgc ggcttctggt
      961 gactcttggc ggcgacgggt cggcaaccgt gccgacactg cagggccttg acctccagcg
     1021 ggccggtctg gttgcccttg acgtccagta cgacctgcgc gatggcgcga ggaacacgtc
     1081 cgccctgcgc acgctgttcg acctgggcct cgacggcgag cgggtcagcc acctcggcgc
     1141 ggctgactgg gccgtgtcgc gctcgcacgc cgacgaagct aaggcgctcg gcatgacggt
     1201 ctacccgcgt ggggtggtcg aggcgcgtgg ggtcgcggca tgcatgtccg ccgcccttga
     1261 ttcggccggg cagagcggcc cggtgcatgt ttccttcgac ctgacggtct gtgatcgttc
     1321 agtcgtcccc ggctgcctcg aagcgttgcc cggcggcctg cgcgccgcgg acctgctgag
     1381 cgcggcgttc attgccggtc gtgatcccaa tgtcagatca gtcgacatcg tcgaggtcga
     1441 tgcaacggct gattccgcgg actaccgcac cgtgcggctc gcggccctcg tcgtcctcga
     1501 ggtggctgct ggcctggcgc tgcgacccca ggcatgaccg cccaggcctt cgcgcacgtc
     1561 gacggccggc tcgcgagcag acttctcgag gtccggcatg accttgcggc gctcgatgag
     1621 ccgggcttct gggtcgtggt cggaggattc gaaggcgagg tcacctgtgc gcggttcgcc
     1681 gacgtccgcg aagcgccgct ccccgccgcg agatggtcag gtccgaagcg cgacgggtgg
     1741 acgacttcac ttgatcaggc cgcctacgtc gagggcgtcg cggcgatccg cgccctgatc
     1801 gcgcagggag cggtctacca ggtcaacctg tgtcgcgtgc tgtctgctca cagcgacgcc
     1861 gccgacctaa gtgggctcgc gaccgtgctg gcagcggagc atccctcgcg ttatccggcg
     1921 ctggtttgcc tgccagaggt gggaattcag atctgctcag cgagccccga gttgttcttg
     1981 tcccgcagcc gcgacgatgg cgtggatgtt gtggcgaccc ggc
//
LOCUS       DASWVF010000058         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_409105, whole genome shotgun sequence.
ACCESSION   DASWVF010000058 DASWVF010000000
VERSION     DASWVF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409105"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGD55_01000"
     CDS             <1..675
                     /locus_tag="VGD55_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868949.1"
                     /translation="FAIITQALAAVLTILIVGQQGYTGGINGITDLRTLLGWDIRTDH
                     AKYVLYFVCCGLLLATMLLARGVIKSKLGRILVATRDKEDRVRFSGYDVANFKVFVFC
                     LAAAMAAIGGAMFTLQVGFMSPSFVGIVPSIDMVIFCAVGGRHSIVGAVIGTLLVNWG
                     KTVFSESFPELWLFAMGGLFIAIVLAFPRGLAGLMTDQILPWIEKLRKTPPGHPDPAT
                     RPAPAE"
     gene            689..1435
                     /gene="urtD"
                     /locus_tag="VGD55_01005"
     CDS             689..1435
                     /gene="urtD"
                     /locus_tag="VGD55_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY0868950.1"
                     /translation="MSSSTDFLLALEDVTVSFDGFKAVNALNLYIDRNELRVIIGPNG
                     AGKTTVLDLICGRTKVTGGSIKFRDQEITGLTEQEIVRRGIGRKFQTPSIYEDLTVFE
                     NLEISVPRGRDVVGALFWKRTPDVVERVRDIARTIFLEEQIDRLAETLSHGQKQWLEI
                     GMLLIQEPELMMLDEPVAGMSISERARTAELLELVSKGRSVIVIEHDMKFVESIADRV
                     TVLHQGKVLAEGSMDVIQNNPQVKEVYLGH"
BASE COUNT          303 a          550 c          501 g          305 t
ORIGIN      
        1 ttcgccatca tcacccaggc gctcgccgcg gtgctgacca tcctgatcgt cggccagcag
       61 ggctacaccg gcggcatcaa cggcatcacc gacctgcgca cgctgctcgg ctgggacatc
      121 cgtaccgacc acgcgaaata cgtgctctac ttcgtctgct gcggattgct gctggcaacg
      181 atgctgcttg cgcggggtgt catcaagagc aagctcgggc gcattctggt ggcgacgcgc
      241 gacaaggagg accgggtccg cttctccggc tacgacgtcg ccaacttcaa ggtcttcgtc
      301 ttctgcctcg ccgcggccat ggccgcgatc ggtggcgcca tgtttacgct ccaggtcggg
      361 ttcatgtcgc cctccttcgt cggcatcgtg ccctcgatcg acatggtgat cttctgtgcg
      421 gtcggcggac ggcattcgat cgtcggcgcg gtgatcggaa cgctcctcgt caactggggc
      481 aagaccgtat tttcggagag ctttccggaa ctctggctgt tcgcgatggg cggcctcttc
      541 atcgccatcg tgctggcctt cccgcgcggc ctcgccgggc tgatgaccga ccagatcctg
      601 ccgtggatcg aaaagcttcg caaaacgccg ccgggtcatc cggacccggc tacccgcccc
      661 gcacccgccg agtaaagaac ccgctgccat gtcctccagc accgatttcc tgctcgcact
      721 cgaagacgtc acggtttcgt tcgacggatt caaagccgtc aacgcgctca atctctatat
      781 cgaccggaac gagctgcgcg tcatcatcgg ccccaacggc gccggcaaga cgacggtgtt
      841 ggacctcatc tgcggtcgca ccaaggtgac cggggggtcg atcaagttca gagaccagga
      901 gatcaccggc ctcaccgagc aggagatcgt gcgccgcggg atcggccgca agttccagac
      961 gccgtcgatc tacgaagacc tcaccgtttt cgagaacctc gaaatctcgg tgccgcgcgg
     1021 ccgcgacgtc gtcggcgcac tgttctggaa acgcacgccg gacgtcgtcg aacgcgtgcg
     1081 cgacatcgcg cggacgattt tcctcgaaga gcagatcgac cgtctcgccg aaacgctgag
     1141 ccacggccaa aagcagtggc tcgagatcgg gatgctcctc attcaggagc cggagctgat
     1201 gatgctcgac gagccggtcg ccggcatgag catctcggag cgcgcccgca ccgcggagtt
     1261 gctcgagctc gtgtccaagg gacgttccgt catcgtcatc gagcacgaca tgaaattcgt
     1321 cgaaagcatc gccgaccgtg tcaccgtgct ccaccagggc aaggtgctgg ctgagggcag
     1381 catggacgtg atccagaaca atcctcaggt caaggaagtc tatctcggcc actaaaagcg
     1441 cccgcccggg gcgttcccgc cggccagggt gtgcgacggt tcccgatcat gtcgaaggag
     1501 caagtccgtg ctcgatgtgc gtaatctcaa cgtcgcctac ggccagagcc aggttctgca
     1561 cggcatcaac ttcgccgtca cgcccggcga aatcgtcgcc gtcgtcggcc gcaacggcat
     1621 gggcaaatcg acgctcatga agtcgctgat cggcgtcat
//
LOCUS       DASWVF010000059         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_128936, whole genome shotgun sequence.
ACCESSION   DASWVF010000059 DASWVF010000000
VERSION     DASWVF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128936"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGD55_01010"
     CDS             complement(<1..405)
                     /locus_tag="VGD55_01010"
                     /inference="COORDINATES: protein motif:HMM:NF045011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868951.1"
                     /translation="MSSLDPVLAELAATHGVATFFHDWVGHERQVSSPAVVSVLAALG
                     VDAGTPEAASRALDDARLARWRRMLPQYLVVRAGSKAQVAVHVPDGAWVEVELELENG
                     GRVALHQLMVWVDPREVDGSLVGVATFEIPDGV"
     gene            complement(408..938)
                     /locus_tag="VGD55_01015"
     CDS             complement(408..938)
                     /locus_tag="VGD55_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0868952.1"
                     /translation="MAYALVLNATYEPIGVVPDRRALLLVLAAKAVSLEHGDAVVHSA
                     SVSVSVPAVVKLTRFVKIPYRTVVPLTRRGVFLRDGGRCAYCDAPATSLDHVVPRSRG
                     GTHTWDNVVSCCRRCNHIKADRTVADLGWRLRRAPRAPSGIAWRILGTGRSDPKWIPY
                     LDGDDNAWAIDQSATA"
     gene            1068..>1656
                     /locus_tag="VGD55_01020"
     CDS             1068..>1656
                     /locus_tag="VGD55_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0868953.1"
                     /translation="MPKPTYPAAHRLDLVEKLPAAMPTFDVADPYRWLEDPVTPDSAA
                     WLEAQDALATAHLDGLPGRPELRTRLTELLAAGVVTAPAWREGRQFFMRRTAEQEHAV
                     LVTIDANGAERVLLDPMQLDPSGTTTLDAWQPSKEGHLLAYQISEGGDEESILRVIDV
                     ETGELIDGPIDRCRYSPVGWLPGAKSYYYVRRLAPS"
BASE COUNT          286 a          610 c          525 g          235 t
ORIGIN      
        1 aacgccgtcc ggtatctcga acgtcgccac gcccaccagc gagccgtcga cttctcgcgg
       61 gtcgacccac accatcagct ggtgcagtgc gacgcggccg ccgttttcga gctcgagctc
      121 gacctccacc caagcgccgt cgggcacgtg cacggcgacc tgcgccttag acccagcgcg
      181 aaccaccaga tattgcggca acatccgccg ccatcgcgcg agccgagcgt catcgagcgc
      241 gcgcgatgca gcctcggggg tgcctgcatc gacgccgagc gcggcgagca ccgacacaac
      301 agcgggggac gacacctggc gctcgtgacc gacccagtca tggaaaaacg tcgccacccc
      361 gtgggtggcc gccagctcgg ccagcaccgg atcgagagaa ctcaccttca ggcggtcgcc
      421 gactgatcga tcgcccacgc gttgtcgtcg ccatcgaggt aggggatcca cttcggatcg
      481 ctccgaccgg tcccgaggat gcgccaggcg attccgctcg gcgcccgcgg cgcacgacgc
      541 aaccgccagc cgaggtcggc gaccgtgcgg tcggccttga tgtgattgca gcgccggcag
      601 cacgacacga cgttgtccca ggtgtgcgtg ccaccccggc tgcgcggcac gacatgatcg
      661 aggctggtgg ccggcgcatc gcaatacgcg catcgaccgc cgtcccgcag gaacacgccg
      721 cgacgagtca gcggcacgac cgtgcggtag gggatcttca cgaaccgcgt gagcttgacc
      781 accgccggaa ccgacaccga caccgacgcg ctgtgcacga cagcgtcgcc gtgttcgagc
      841 gagacggcct tcgcagccag cacaagcaag agggcgcgac gatccggtac gacgccgata
      901 ggttcatacg tcgcattcag aacgagcgcg taagccactg ggcctcctcg tcccctcccc
      961 gctgtccccg acagtctcgc cggtcggacc acccccgcgc cagctattca acccgcgcgc
     1021 cgcgacgctg caacgattgt gacctccggc ttggttaggg tcgtggcatg ccgaagccga
     1081 cgtaccccgc cgcccaccgt ctcgacctgg tggagaagct gcctgcggca atgccgactt
     1141 tcgacgtcgc agacccctac cgctggctcg aggaccctgt aacgccagac tccgcggcct
     1201 ggctcgaagc acaagatgcg ctcgccaccg cgcatctcga cgggctgcca gggcgcccgg
     1261 agttgcgcac tcgcctgacc gagctcctcg ccgccggcgt ggtgactgca cctgcgtgga
     1321 gagaaggtcg ccagttcttc atgcggcgca ccgccgaaca ggagcacgcg gtgctcgtga
     1381 ccatcgacgc caacggcgcc gagcgggtgc tgctcgatcc gatgcagctc gacccgtccg
     1441 gcacgaccac tctcgatgca tggcagccct cgaaggaggg gcacctcctc gcctaccaga
     1501 tctccgaagg cggcgacgag gagtcgatcc tgcgggtgat cgacgtcgag accggtgagc
     1561 tgatcgacgg accgatcgac cgctgccgct actcccccgt cggctggctg ccgggggcga
     1621 agtcgtacta ctacgtgcgg cgactcgctc cgtctg
//
LOCUS       DASWVF010000060         7862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_77949, whole genome shotgun sequence.
ACCESSION   DASWVF010000060 DASWVF010000000
VERSION     DASWVF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77949"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VGD55_01025"
     CDS             <1..631
                     /locus_tag="VGD55_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0868954.1"
                     /translation="HNDAPCFAAAEHWLGAAKGVDNMLGMVVSTGVGAGVVIGGQLVD
                     GASGNAGHIGHVVVDPVGPPCSCGGRGCLEAIARGPALAAWAVQNGWRASGDGSARAL
                     ADDARGGDPVAVAAFARAGRAVGTAVASAAALLDITLVVVGGGVAQAGELLFAPARRA
                     LRDHSGVEFLRDLKIVPSALGPDAGLVGAAALVLDGPHYWPSEVGAGGG"
     gene            624..1679
                     /locus_tag="VGD55_01030"
     CDS             624..1679
                     /locus_tag="VGD55_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0868955.1"
                     /translation="MGDSTPEQLDPIDALRQIAFLLERAREPTYRVKAFRTAAAVLAA
                     LPPGDLEARVKRGTLTDLPGVGQATATVVREALTGVVPAYLAKLLPAGSQALVQGGEQ
                     LRAALLGDLHTHSDWSDGGSPIEEMAVAARDLASGPHSYIVLTDHSPRLTVANGLTAD
                     RLREQLRVVAALNEKLTPFRILTGIECDILDDGSLDQEPELLDELDIVVASVHSKLAM
                     DAAAMTRRMLAAVRNPAVDVLGHCTGRLLIGREGGKPRPESHFDAEKVFAACKEHGVA
                     VEINSRPERLDPPRRLLTIAVEIGCQFSIDTDAHAPGQLDWQIYGCARAQECGVDASR
                     VVNTMDADALLARLARR"
     gene            1689..2225
                     /locus_tag="VGD55_01035"
     CDS             1689..2225
                     /locus_tag="VGD55_01035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0868956.1"
                     /translation="MPLPGPIVRDATDRDAQECAAIYALYVTDTAITFEEVPPSPAEM
                     AQRIVTAARAHAWLVLEDDGRVVGYAYGRSFNPRAAYRWTCEVSVYLEHGRRRTGAGR
                     ALYEALLARLVQRGFRTAVAAMTLPNDASVGLHASMGFAPVGILRRVGWKHGAWHDVA
                     WVQRELAADLSDAPAEPT"
     gene            2222..3409
                     /locus_tag="VGD55_01040"
     CDS             2222..3409
                     /locus_tag="VGD55_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY0868957.1"
                     /translation="MTVWHGLLHALGIAGSMTWQVTWSLVLGFTLSAIIESLVKKSTI
                     ARLLPDDRPRSLATATALGAASSSCSYAAVALARSLFRKGADFTAAMAFQFASTNLVI
                     ELGLILALLIGWQFTLAEFVGGPFMILVLALIFRRFLTTKLRANAREQAERAIAGSME
                     GHAAMDMSVAAGGSFLRRLASPAGFTSVSHNFVMEWAAVLRDIVIGLLIAGCAAAWIP
                     DSFWQHLFFTSHPLLAKIWGPLIGPLVAVAAFVCSIGNVPLAAVLWNGGISFGGVVSF
                     IFADLIIIPILLIYAKYYGRAMAWFLFGTFYLTMVSAGYFIELVFTPLHLVPTGKRHA
                     SVAEHGITWNYTTALNIAFLLVAAALVWRFLTTGGREMLAMMGGGPDDMDHHGHAHEE
                     GHG"
     gene            complement(3427..4311)
                     /locus_tag="VGD55_01045"
     CDS             complement(3427..4311)
                     /locus_tag="VGD55_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0868958.1"
                     /translation="MRTQLAPGTADHVVEGIRIRFTTYGGPGDGLDGSPAVLLLHGFP
                     TSSQLWRNVGPEMCAGTEDDLFVLAPDLVQLGESERTGVRIDLATQARLLLGLLDDKG
                     IDKVIVAGHGLGGAVAVHLAALDGRRVAGLALLDTPLHADAWPVAGALPMLIPGVRSI
                     CGRALPVVPWLAQRVLATLVGSGSDAADLRPLDRYVDQLRRPGAARGLVHFATAVDPV
                     AIEAAWHVVRASPPPTLIMWGAEDRVHNIAYGRRVAGEIPSAAWVPVAGAGHLLPEER
                     PERVAEELVAFHAEISGD"
     gene            4392..5624
                     /gene="ilvA"
                     /locus_tag="VGD55_01050"
     CDS             4392..5624
                     /gene="ilvA"
                     /locus_tag="VGD55_01050"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0868959.1"
                     /translation="MTGSFEPRDARAWLDDARAAAVVIDGVARRTPIESSRALAELVG
                     GGVRLKCENLQRGGSFKIRGAYVRISRLTDAQRAGGVVAASAGNHAQGVALAASLLGA
                     AATVFMPEGAPLPKVEATLKYGADVRFFGHSVDEALTAAAAFAAETGAVLIHPFDHPD
                     IVAGQATAGLEILEQCPDVKTIVVSTGGGGLTAGIAVVVKALRPDVRVVGVQAGGAAA
                     FPASLEAGTPMRLASMSTIADGIAVGCPGELTFALVRDYVDQIVTVSEDSLSRALLFC
                     LERAKLVVEPAGAAAVAAIIDAPQSFEPPVVAVLSGGNVDPVLLIGVLRHGLAAAGRY
                     LSFEVTISDRPGGLAALLQTLAVAGGNVLDVVHERTAPTLHLDEVVVALQVETRGPTH
                     SREVVEMLRTHGYRVAFT"
     gene            5700..6746
                     /locus_tag="VGD55_01055"
     CDS             5700..6746
                     /locus_tag="VGD55_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0868960.1"
                     /translation="MVEVQATPRAPRPTGGAIVAQGLVKHYGKVRALDGVDLQVPEGT
                     VLGLLGPNGAGKTTAVRVFTTLLKPDAGTATVAGLDVVRDANEVRRIIGVSGQYAAVD
                     ENLTGRENLDMVGRLYHLGKAISAARADELLERFDLVEAANRVVKTYSGGMRRRLDLA
                     GSLVARPKVLFLDEPTTGLDPRGRLGMWDVIEELVDGGTTLLLTTQYLEEADRLAQSI
                     VVIDHGKVIARGTADELKSQIGGERLELTVSTAAELPATELVLRKVGFGEPAIDAGTR
                     AASIAVQGGSAVLIDALRQLDAAGVVVKDVGIRRPTLDDVFLSLTGHAAEDAADPASA
                     GAKPGRRARKEKQA"
     gene            6743..7543
                     /locus_tag="VGD55_01060"
     CDS             6743..7543
                     /locus_tag="VGD55_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0868961.1"
                     /translation="MSVLTQATTDGLVMAKRNLIKVKRIPDLLIFTTIQPVMFVLLFA
                     YVFGGAIHPKGVNYREFLMAGIFTQTVAFGSAITAIGLADDLQKGIIDRFRSLPMARS
                     AVLVGRTTSDLFNNVIVMIVMSICGLLVGWRIHRGFGYALGAYGIMFAFAFAMSWVSA
                     TIGLSVRSVEVAQSAGFIWLFPLTFMSNAFVPTDTLPGWLQAVANWNPMSAVVAAARA
                     LFGNQGTAAIPKAWSMQHPEITALGWSLLLLVIFIPLSVRKYRAAAAR"
     gene            complement(7553..>7862)
                     /locus_tag="VGD55_01065"
     CDS             complement(7553..>7862)
                     /locus_tag="VGD55_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY0868962.1"
                     /translation="MEARLRQLRQLLDNAKVGAGPADDGLVGPGMVVTVRYEGDADTL
                     TFLLGSREDAGGGIDVYSPSSPLGAALLGKSAGDTVSYALPNGTKLAVEILEAKPYTG
                     "
BASE COUNT         1221 a         2552 c         2682 g         1407 t
ORIGIN      
        1 gcacaacgac gctccctgct tcgcagccgc cgagcactgg cttggggccg ccaagggggt
       61 cgacaacatg ctcggcatgg tcgtctcgac gggtgtcggc gccggcgtgg tcatcggagg
      121 ccagcttgtc gatggcgcca gcggcaatgc cggtcacatc ggccacgtag tcgtcgaccc
      181 ggtcggtccg ccatgttcgt gtggtggtcg aggctgcctt gaggcgatcg cgcggggccc
      241 ggcgcttgct gcgtgggctg tgcaaaacgg ctggcgggcg tcgggcgacg gcagcgcgcg
      301 tgcactcgcc gacgacgcac gcggcggtga tccggtggcg gtcgccgcat tcgcgcgagc
      361 cggacgtgcc gtcggcaccg ctgtcgcctc agctgctgca ctgctcgaca tcactctggt
      421 cgtggtcggg ggcggcgtcg cgcaggccgg cgagttgctg ttcgcgccgg ctcgccgcgc
      481 ccttcgcgat cattcaggcg tcgagttttt gcgcgacctc aagatcgtgc cttcggcact
      541 ggggccggac gccgggctgg tgggcgccgc cgcgctggtg ctcgacggcc cgcattactg
      601 gccgtcagag gtgggagcgg gcggtgggtg actcgacgcc ggaacagctc gacccgatcg
      661 acgcgcttcg ccagatcgcc ttcctgctcg agcgcgcgcg tgaaccgacg tatcgcgtga
      721 aggccttccg cacggcagcc gcggttctcg ccgcgctgcc gccgggcgat ctcgaagcgc
      781 gggtgaagcg tggcaccctt accgacctgc cgggtgtcgg tcaggcgacc gcaaccgtgg
      841 tgcgcgaggc gctgaccggt gtggtgcccg cctatcttgc gaagctgctc cctgcgggtt
      901 cgcaagccct ggtgcagggt ggcgagcagc tgcgggccgc cttgctcggc gatctgcaca
      961 cgcactccga ctggagcgat ggcggcagcc cgatcgagga gatggcagtc gctgcgcgcg
     1021 atctcgccag cggcccccat tcctacatcg tgctgaccga ccattcaccg cgactgacgg
     1081 tcgccaacgg ccttaccgcc gaccgattgc gcgaacagtt acgtgtcgtg gcggccctga
     1141 acgagaagct cacgccgttt cggatcctga cgggcatcga gtgcgacatc ctcgacgacg
     1201 gttcgttgga tcaggagccc gagttgctcg acgaactcga catcgtggtg gccagcgtgc
     1261 actccaagct cgcgatggac gccgccgcga tgacgcgacg gatgctggcc gccgtccgta
     1321 accctgctgt tgatgtgctc ggacattgca cgggccggct gctgatcggg cgcgagggtg
     1381 gcaaaccgcg gcccgagtcc cacttcgacg ccgagaaggt ctttgccgcg tgcaaagagc
     1441 acggtgtcgc ggtcgagatc aacagccgcc cagaaaggct tgatccgccg cggcggctgt
     1501 tgacgatcgc ggttgagatc ggctgccagt tctcgatcga taccgacgcg cacgcgcccg
     1561 ggcagctcga ctggcagatc tacggctgcg cgcgggcgca ggagtgcggc gtcgatgcgt
     1621 cgagggtcgt caacacgatg gacgccgacg ccctgcttgc caggctcgct cgtcgctagc
     1681 atcgctgcat gcccctgcct ggcccgattg tccgcgacgc gacagatcgc gacgcgcagg
     1741 agtgtgcggc gatctatgcc ctgtacgtca ctgacacggc gatcaccttc gaggaggtgc
     1801 cgccgtcgcc cgccgaaatg gcgcagcgaa tcgttactgc tgcacgcgcg catgcgtggc
     1861 tggtgcttga agacgacggg cgtgtcgtcg gctatgccta cggtcgctcg ttcaacccgc
     1921 gcgcggcgta ccggtggacg tgcgaggtga gcgtctacct cgagcacggc cgtcggcgaa
     1981 cgggcgcggg gcgcgcgctg tacgaggcac tgctcgcgcg attggtgcag cgcgggtttc
     2041 gcactgctgt cgccgctatg acgctaccca atgacgcaag cgtcggcctg cacgcgtcga
     2101 tggggttcgc gccggtcggg atcctccgac gggtcggctg gaaacacggc gcgtggcatg
     2161 acgtggcctg ggtccagcgg gagctcgctg ctgatctaag cgacgcacca gcggaaccga
     2221 cgtgaccgtc tggcacggct tgctgcacgc actcggcatc gccggctcga tgacctggca
     2281 ggtcacgtgg tctttggtgc ttggcttcac gttgtcagcg atcatcgagt cgctggtcaa
     2341 gaagtcgacg atcgcccggt tgctgcccga cgatcggccg cgcagcctcg ccaccgcgac
     2401 agcgctcggc gctgcatcgt cgtcgtgctc ctatgccgcc gtggcgcttg cccggtcgct
     2461 gtttcgcaag ggcgccgact tcacagcggc gatggcattc cagttcgctt cgacgaacct
     2521 tgtcatcgaa ctcggactca tcctggcgtt gctgatcggg tggcaattca cgctcgcaga
     2581 attcgtcggc gggcctttca tgattctcgt tctcgccttg atcttccgcc ggttcctcac
     2641 caccaagttg agagcgaacg cacgcgagca ggcggaacga gccatcgccg ggtcgatgga
     2701 agggcacgcc gcgatggaca tgtcggtcgc ggccgggggc tcattcttgc gacgacttgc
     2761 gagtcccgcc gggttcacgt ccgtcagtca caacttcgtc atggaatggg cggcagtcct
     2821 tcgtgacatc gtcatcgggt tgttgattgc aggttgcgct gcggcgtgga ttccggacag
     2881 cttctggcag cacctgttct tcaccagcca cccgttgctg gccaagatct gggggccact
     2941 gatcgggccg ctggtagccg tggcggcatt cgtctgctcg atcggcaacg tgccactggc
     3001 tgcagtgctg tggaacggcg gcatcagctt cggaggagtc gtcagcttca tcttcgccga
     3061 cctgatcatc attccgatcc tgctgatcta cgcgaagtac tacggccggg cgatggcgtg
     3121 gttcctattc ggcacgttct acctgacgat ggtgagcgcc gggtacttca tcgaactcgt
     3181 gttcaccccg ctgcatctcg tcccgaccgg caagcggcac gcgtccgttg ccgaacacgg
     3241 catcacgtgg aactacacga ccgcgctcaa catcgcgttt ctcctcgtcg ccgctgcact
     3301 tgtgtggcgc ttccttacga ccggagggcg agagatgctg gcgatgatgg gtggcgggcc
     3361 tgatgacatg gaccaccacg gccacgccca cgaagaaggg cacggctagc accggccacc
     3421 tgactgctag tcgccggaga tttctgcgtg gaatgccacg agttcctcgg cgacccgttc
     3481 gggccgttcc tcggggagca gatgccctgc gccggccacc ggaacccagg cagcgctggg
     3541 aatttcgcca gcaactcgtc gtccgtacgc gatgttatgc acccgatctt cggcgcccca
     3601 catgatcaat gtgggtggcg gcgacgcccg aacgacgtgc cacgcagcct cgatggcgac
     3661 ggggtcgacg gctgtcgcga aatgcacgag cccgcgcgcg gcaccaggcc tccgcaattg
     3721 gtcgacgtag cgatcgagtg gccgaagatc tgcggcgtca ctgccgctac cgaccagcgt
     3781 cgcgagcacg cgctgcgcca gccacgggac gacgggaagt gctcgtccgc agatcgagcg
     3841 cacgccagga atcagcatcg gtagcgcgcc cgccaccggc cacgcatcgg cgtgcaacgg
     3901 cgtatcgagc aacgcgaggc ccgccacccg acgtccgtca agagccgcga gatgcaccgc
     3961 gaccgcacca ccaaggccgt gtccggcaac gatcaccttg tcgatgccct tgtcgtcgag
     4021 caggccgagc agcaagcggg cttgggtcgc aagatcgatg cggacgccgg ttcgttccga
     4081 ctccccgagc tgcacaagat cgggggccag cacgaaaaga tcgtcctcgg tgcccgcgca
     4141 catctccggc ccgacgttgc gccacagttg cgacgacgtc gggaatccgt gcagcagcag
     4201 cactgcgggt gagccgtcga ggccatcacc agggccgccg taggtggtga accggatccg
     4261 gatgccctcg acgacgtggt cggccgtgcc tggggcgagt tgggtgcgca tttgttcatc
     4321 ctgcaccgga tagcgtggcc gaaacgtggc gagggggagg caatcttgtc tccaggtgcc
     4381 agacttcgcg catgaccggc tcgttcgaac cgcgcgacgc tcgcgcctgg ctcgacgacg
     4441 cgcgcgcggc agctgtggtc atcgacgggg tcgcgcgccg tacaccgatc gagtcctcgc
     4501 gcgcgcttgc cgagctggtc gggggaggcg tccggctgaa atgcgaaaac ttgcagcgcg
     4561 gaggatcgtt caagatccgc ggcgcctacg tgcgcatctc gaggctcacc gacgctcaac
     4621 gcgcgggcgg ggtcgtggca gccagcgccg gcaatcacgc acagggtgtt gcgctcgccg
     4681 cgtcgttgct gggcgcggca gcgacggtgt tcatgcccga gggcgccccg ctaccgaagg
     4741 tcgaagccac cctgaagtac ggcgccgacg tgcgtttttt cggtcatagc gttgacgaag
     4801 cactcaccgc cgcagccgcg ttcgccgccg aaactggggc ggtgctgatc cacccgttcg
     4861 accaccccga catcgtggcg gggcaagcga ctgcaggcct ggaaatcctc gagcagtgcc
     4921 ctgacgtgaa gacgatcgtc gtcagcaccg gcggcggcgg attgaccgca ggcatcgccg
     4981 tcgtagtcaa ggcattacga cccgatgttc gagtggtcgg cgtccaagcc ggcggtgcgg
     5041 ccgcgtttcc cgcatcgctt gaggccggga cgccgatgcg gctggcctcg atgtcgacga
     5101 tcgccgacgg catcgcggtc gggtgtcctg gtgagctcac gttcgcgctg gtacgcgact
     5161 acgtcgatca gatcgtgacg gtctcagaag actcgctgtc aagggcgctg ttgttctgcc
     5221 tcgagcgcgc gaagcttgtt gtcgagccgg ccggcgcggc tgccgtcgcc gcgatcatcg
     5281 acgcgccgca atcgttcgag cccccggttg tcgccgtctt gtctgggggc aacgtcgacc
     5341 ccgtcctctt gatcggcgtt ctgcggcacg ggctcgcggc cgcgggccgc tacctttcgt
     5401 tcgaggtgac gatcagtgat cggccaggag ggctcgccgc tctgctgcaa accctcgcag
     5461 tggccggggg gaacgtgctc gatgtggtgc acgagcggac ggcaccgacc cttcacctcg
     5521 acgaggtcgt ggtggctctc caggtcgaga cccgtgggcc gacgcactcg cgcgaggtcg
     5581 tcgagatgct gcgcacccac ggataccggg tcgcgttcac ctaacacggt cgatagcctg
     5641 ctttgctgcc ggtctgaatg gaccgatgac ttcagcggct cgaaggagtc tccctttaca
     5701 tggtcgaggt tcaagcaact ccgcgcgctc cgcggcccac aggtggcgcg atcgtggcgc
     5761 agggcctggt caagcactac ggcaaggtgc gggcgctcga cggggttgat ctgcaggtgc
     5821 ccgaggggac cgtgctcggc ttgctcggcc cgaacggtgc cggcaagacg accgcggtgc
     5881 gggtattcac gacactgctg aagccggacg ccggcacggc gaccgttgcc ggtctcgatg
     5941 tcgtgcgtga cgcgaacgag gtacgccgca tcatcggggt gtcggggcaa tacgcggcgg
     6001 tcgacgaaaa cctgaccggt cgcgagaacc tcgacatggt gggccggctc tatcacctcg
     6061 gcaaggcgat ctcggctgct cgcgccgacg agttgctcga acgattcgac ctggtcgagg
     6121 ccgccaatcg cgtggtgaag acctactccg gcggcatgcg ccgcaggctc gacctcgcgg
     6181 gcagcttggt ggcccgaccg aaggtcctgt tcctcgacga acccacgacc ggcctcgacc
     6241 cgcgcgggcg cctcggcatg tgggacgtca tcgaagagct cgtcgacggc ggtacgacgc
     6301 tgctgctcac aacgcagtac ctcgaggaag ccgatcggct tgcccagtcg atcgtggtga
     6361 tcgatcacgg gaaggtgatc gctcgcggta ctgccgacga actgaagtcg cagattggtg
     6421 gcgaacgact cgagctgacg gtgtcgaccg ctgcggagtt gccggctacg gaactggtct
     6481 tgcgcaaggt cgggttcggc gagccggcga tcgacgcggg caccagagcg gcctcgattg
     6541 cggtgcaggg tgggtcagcg gtgctgatcg atgctctacg ccagctcgat gccgcgggag
     6601 tcgtggtcaa ggacgtcggc attcggcgtc cgactctcga cgatgttttc ttgtcattga
     6661 ccggccacgc ggccgaagac gccgccgatc ccgcatcggc cggcgcaaaa ccgggccgtc
     6721 gcgcacgcaa ggagaagcag gcatgagcgt cttgacgcag gcgaccaccg acggtctggt
     6781 gatggcgaag cgcaacctca tcaaggtgaa gagaatccct gacctgctga tcttcaccac
     6841 gatccagccg gtgatgttcg tgctgctgtt cgcttacgtg ttcggcggcg cgatccaccc
     6901 gaagggcgtg aattaccgcg agttcctgat ggccggcatc ttcacgcaaa ccgtcgcgtt
     6961 cggctcggcg atcaccgcga tcggtcttgc tgacgatctt cagaagggca tcatcgatcg
     7021 ctttcgatcg ctgccgatgg cgcgctcggc agtgctcgtt ggtcgcacga cgtccgacct
     7081 gttcaacaac gtcatcgtca tgatcgtgat gtcgatctgc gggctgcttg ttggatggcg
     7141 catccaccgc ggtttcggct acgcgctcgg ggcatacggc atcatgttcg cttttgcgtt
     7201 cgcgatgtcg tgggtgtcgg cgaccattgg cttgtcagtg cgcagcgtcg aggtcgcgca
     7261 gagcgccggc ttcatctggt tgttcccgct cacgttcatg tcgaacgcat tcgtgccaac
     7321 cgacacgctt cctggctggt tgcaggcggt cgcgaactgg aatccgatga gtgcagtcgt
     7381 cgccgccgca cgagcgctgt tcggcaacca gggcaccgca gcgattccga aggcgtggtc
     7441 gatgcagcat ccggagatca ccgcgctcgg ctggagcctg ctgctgttag tgatcttcat
     7501 tccgctgtcg gtgcgcaagt atcgggccgc cgccgctcgc tagggcggtg cgtcagccgg
     7561 tatagggctt cgcttcgagg atctcgactg ccagcttcgt gccgttcggc aacgcgtacg
     7621 agacggtgtc gcccgcactc ttgccaagga gtgcggctcc cagtggcgaa ctcggggagt
     7681 agacgtcgat gccgccgccg gcgtcttcgc gcgagccaag caggaatgtc aacgtgtcag
     7741 cgtcgccctc gtagcgaacg gtgacgacca tgcccggccc gacgaggccg tcgtcggccg
     7801 gacccgcgcc gaccttggcg ttgtcgagaa gctgccgaag ctgccggagg cgggcttcca
     7861 tc
//
LOCUS       DASWVF010000061         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_27045, whole genome shotgun sequence.
ACCESSION   DASWVF010000061 DASWVF010000000
VERSION     DASWVF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27045"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VGD55_01070"
     CDS             complement(<1..783)
                     /locus_tag="VGD55_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0868963.1"
                     /translation="MRRMKGAAIAVTAGLLAAACGSSGHKASTATPSAAASSPTANTS
                     SSAPATTSAPATSSAPASVAPASSAAPTYKQGGTVTISNEQGQTWTCQFNPYNPAVNA
                     ESLGFFYEPLIYVDLLNNSAETPMLATGYQWAADKKSIVFTIRDGVKWNDGQPFSAAD
                     VAFTFNLMKKVPALDLYALWSDAGLQSVTASGNQVTMTFSQVASTYFFNFADQVGIVP
                     EHIFSTGDAAAHPDTWADPNPVGTGPFTVSPCTPNNIQMVANP"
     gene            complement(881..1813)
                     /locus_tag="VGD55_01075"
     CDS             complement(881..1813)
                     /locus_tag="VGD55_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0868964.1"
                     /translation="MTRSPVILGLDFGGTKIAMAICDQSGNRVADTVLATEAEAGAAS
                     AFERAITAARDLLLTAAPGAELIAVAAATFGIPFEDRVELAPAIPGWESLRFGHELRA
                     AFPDVLLCMATDVKAAALAEVRWGALAGADPAIYLNLGTGLATAIVAGGKVVNGAHGA
                     AGEIGYNLREPADVGRPNSERLTLEDVVSGKALAEIARAELGRRLSTAELFDLAETDE
                     AAARIVEAFIRELAQHVVNLAVAVDPARIAVGGGLARSWNVLAPVLRGALDAAVPYPP
                     ELVLAAFPFDAPLIGALALGVEAAAAVLGEGALR"
BASE COUNT          353 a          673 c          702 g          357 t
ORIGIN      
        1 cgggttcgcc accatctgga tgttgttcgg ggtgcacggg cttaccgtga acgggccggt
       61 gccgaccggg ttcgggtctg cccaggtgtc ggggtgcgcc gccgcgtcac ccgtggagaa
      121 gatgtgctcg ggcacgatgc cgacctggtc ggcaaagttg aagaagtagg tcgaggcaac
      181 ctgcgagaac gtcatggtga cctggttgcc gctggccgtc acgctctgga gacccgcgtc
      241 gctccacagc gcataaaggt cgagcgccgg aaccttcttc atgaggttga atgtgaacgc
      301 aacgtcggct gcgctgaacg gctgaccgtc gttccacttc acaccatcgc gaatcgtgaa
      361 cacgatcgac ttcttgtcag ccgcccactg gtagcccgtg gcgagcatcg gggtctcggc
      421 cgagttgttc agcagatcga catagatgag tggctcgtag aagaagccga gcgactctgc
      481 gttgaccgcc gggttgtacg ggttgaactg gcaggtccag gtctggccct gctcgttgga
      541 gatcgtgacc gtgccgccct gcttataggt cggcgcggcg ctgctcgcgg gagcaacgct
      601 cgcaggcgca ctgctggtgg ccggcgcgct ggtcgtggcg ggcgcgctcg agctcgtgtt
      661 cgcggtcggc gaagacgctg ccgccgatgg tgtagccgtg cttgctttgt ggccgctact
      721 gccgcaagcg gcggccaata ggccggccgt cactgcgatg gcggcacctt tcattcgcct
      781 catggtgtta gcccctcact cagttgtgcg gcaggcgctg cgcctgtcgg ataaacggct
      841 ctccggattt gtcgatcgcc aagactgtgc tcaggtcatg tcacctgagc gccccctctc
      901 cgaggacggc agctgccgcc tcaacgccca gtgcaagcgc accaatcagc ggtgcgtcga
      961 acgggaacgc cgcgagaaca agttcgggtg ggtaaggcac ggcggcgtcg agcgcaccgc
     1021 gcaacaccgg cgcgagcacg ttccacgaac gtgccaagcc gccgccgacc gcgatcctcg
     1081 ccgggtcgac tgcaacagcg aggttgacga cgtgctgcgc cagctcgcgg atgaacgcct
     1141 cgacgatgcg ggcagcggct tcgtctgtct cagcgagatc gaacaactcc gccgtcgaaa
     1201 gccggcgacc gagctctgcg cgggcgatct cggcaagcgc cttgccgctg acgacatctt
     1261 cgagggtgag gcgctcggag ttcggccgac cgacgtccgc cggttcacgc aggttgtaac
     1321 cgatctcgcc cgccgcaccg tgcgcaccgt tgaccacctt gccgcccgcg acgatcgcgg
     1381 tcgcgaggcc ggtgcccaga ttgaggtaga tggccggatc ggcgccggca agcgcacccc
     1441 agcgcacctc cgcgagcgcc gctgccttga cgtcggttgc catgcacaac agcacgtcgg
     1501 gaaacgcggc gcgcagctcg tgaccgaacc gaagagactc ccagccgggg atcgccggtg
     1561 ccagctcgac gcggtcttcg aacggaatcc cgaaggtggc tgcagcgacg gcgatgagtt
     1621 cggcccctgg tgcagctgtg agcagcaggt cgcgtgcggc ggtgatcgcc ctctcgaacg
     1681 cgctggccgc acccgcctcg gcctcggtcg caagcaccgt gtcggccacc cggttgcccg
     1741 actggtcaca gatggccatc gcgatcttgg tgccgccgaa atcgaggccc aggatgaccg
     1801 gcgaacgagt cacaggtggg agcctctcag gggttgtgga agccgggctg gctaagattg
     1861 ttaggaaagt aacctaccga ttcgtttgca cgcaagactg gttggtaaca gcttcagtac
     1921 gatcgcggca ctcgaggaag gcccggagat gacgctgctc ggtagtggga tgtcggccgc
     1981 gcgcccacag ctgatccgga ccatgaacga aaacgtgctc atcgactaca tccgccgcac
     2041 cggcccactg tcgcgagccg acctcgcccg cagctctggg ttgtc
//
LOCUS       DASWVF010000062         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_154508, whole genome shotgun sequence.
ACCESSION   DASWVF010000062 DASWVF010000000
VERSION     DASWVF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154508"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1633
                     /gene="der"
                     /locus_tag="VGD55_01080"
     CDS             110..1633
                     /gene="der"
                     /locus_tag="VGD55_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0868965.1"
                     /translation="MIDKGRIDNPGDTVEGADDHDAGTWVSEADWVGLSDDDLAVADG
                     SADGVDDVDDEPFVVPLVAIVGRPNVGKSTLVNRILGRREAVVEDVPGVTRDRVMYDA
                     NWRGRAFTVVDTGGWEPDALGLQAQVSAQAEVGAFAAQVVVMVVDATVGATETDEAVA
                     RVLRRARKPVVLVANKVDTARGESDASSLWSLGLGEPYAVSALHGRGSGELLDAIFEA
                     LPELPPDEGLRREGPRRVALLGRPNVGKSSLLNKVSGEDRSVVDAVAGTTRDPVDSLV
                     TLAGVTWRFIDTAGIRRRVREASGAEYYSSLRTATALREAEVAVVLLDASEPLAEQDL
                     RIIDMVVESGRALVLAFNKWDLLDEDRHDVLEKEIDRQLHHVAWAPRVNVSAKTGRAV
                     DKLTPALEVALEGWSQRVPTSKLNAWLSALIAATPPPVRGGRQPRVLFATQAGTQPPR
                     FVLFSSGFLEATYRRFIERRLREDFGFPGTPVEVAVRVKESRKGKPRGVRNRTGRTS"
     gene            1680..1753
                     /locus_tag="VGD55_01085"
     tRNA            1680..1753
                     /locus_tag="VGD55_01085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1714..1716,aa:Pro,seq:ggg)
BASE COUNT          319 a          545 c          637 g          332 t
ORIGIN      
        1 tcgccgcgat tctcgacgtc gtcgccaaaa ggaccggcac aacctcggac ggttcgcgac
       61 ccgacgagat tcatgactcg cgcgtcgacc aggtaaacgg tgagcgcaga tgatcgacaa
      121 aggtcgtatc gacaatccag gcgacaccgt tgaaggcgcc gacgatcacg acgcggggac
      181 gtgggtaagc gaggccgact gggtcggtct cagcgatgat gatctcgctg ttgcggacgg
      241 cagcgcggac ggcgtcgacg acgttgatga cgaaccgttt gtggttccgc tcgtcgcgat
      301 cgtcggtcgg ccaaacgtcg gaaaatccac gctggtcaac agaattctcg gccgccgtga
      361 agccgttgtc gaagatgtgc ccggcgtcac gcgtgatcgc gtcatgtacg acgcgaactg
      421 gcgtgggcgt gcgttcaccg tggtcgacac cggtggctgg gagcccgacg ccctcggctt
      481 gcaagcccag gtttcggctc aggctgaggt cggtgcattc gcggcccagg tcgttgtgat
      541 ggtggtcgat gcaacggtcg gtgccaccga aaccgacgag gccgtagccc gggtacttcg
      601 cagggcacgc aaacccgtgg tactcgttgc gaacaaggtc gatacagcgc gcggcgagtc
      661 agacgcgtcg agtctgtggt cgcttggcct cggcgagcct tacgccgtca gcgccttgca
      721 tggccgtggt agtggcgagc tgctcgacgc catcttcgag gcgctcccgg agctacctcc
      781 cgacgagggc cttcgacgcg aagggccacg gcgcgtcgct ttgctcggac gcccgaacgt
      841 cggcaaatcc agtctgctga acaaggtgtc aggcgaagac cgctcggtgg tcgacgcagt
      901 tgccggaacg acgcgagatc cggtcgactc gctggtcacg ctggcgggtg tgacgtggcg
      961 tttcatcgac acggcaggga ttcgcaggcg ggttcgcgag gccagcggtg ccgagtacta
     1021 ctcgagcctg cggacggcga cggctttgcg agaagcggaa gtggccgtcg tcctgcttga
     1081 tgcaagtgaa ccgttagccg aacaagacct gcggattatc gacatggtcg tcgaatcggg
     1141 ccgtgcgctg gttctcgcgt tcaacaagtg ggatttgctc gacgaggatc gccacgacgt
     1201 actcgagaag gagatcgacc ggcaactgca tcacgtggcg tgggcgccgc gggtgaacgt
     1261 gtcggcgaag accgggcgtg ccgtcgacaa actcaccccg gctttggagg ttgcgctcga
     1321 gggctggtcg caacgagtgc cgacctcgaa actgaatgcg tggctcagcg cgctgatcgc
     1381 tgcgacccca ccacccgtgc gcggagggcg gcagccgcgc gtgcttttcg cgacccaagc
     1441 tggcactcag ccgccgcggt tcgtcttgtt ctcgtcgggt tttcttgagg cgacgtaccg
     1501 gcgtttcatc gaacgacgtc tgcgcgagga tttcggcttt cccgggactc ctgtcgaggt
     1561 cgcggtgcgc gtcaaagagt cgcgcaaggg caagccgcgc ggcgtgcgaa atcgcactgg
     1621 ccgaacgtcc tgacagtgcc ggtgcgatat cgtcggcagg cccgacgaaa accgggcgcc
     1681 gggatgtggc gcagcttggt agcgcacttg actgggggtc aaggggtcgc aggttcaaat
     1741 cctgtcatcc cgacggcgtg agttcgcacg gtatcggcgg cctcgaacct acgggttcga
     1801 ggttttcgca gtttcggaag tcggccgtca cgc
//
LOCUS       DASWVF010000063         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_460214, whole genome shotgun sequence.
ACCESSION   DASWVF010000063 DASWVF010000000
VERSION     DASWVF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460214"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGD55_01090"
     CDS             complement(<1..397)
                     /locus_tag="VGD55_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY0868966.1"
                     /translation="MQQQHERVDEPLRLGDRGLAVLELRHRLRQLGLLINDETRAGQS
                     SEAADLFDEACDHAVRSFQQQRGLRIDGVVDRETYRGLDEARWRLGDRTLSFAVRHPY
                     VGDDVAGLQHRLTDMGFDCGRADGVFGPRT"
     gene            complement(511..834)
                     /gene="trxA"
                     /locus_tag="VGD55_01095"
     CDS             complement(511..834)
                     /gene="trxA"
                     /locus_tag="VGD55_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0868967.1"
                     /translation="MGATKSVTDETFATDVLNSDTPVLVDFWAEWRGPCRMVAPVLEE
                     IANEHADKVRVVKLNIDENPETARAYSILSIPTLNVYKGGQVVKQIIGAKSKSALLKD
                     LEAFL"
     gene            complement(918..>1634)
                     /locus_tag="VGD55_01100"
     CDS             complement(918..>1634)
                     /locus_tag="VGD55_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0868968.1"
                     /translation="ARFGAELVTDDVTAVDLTGPIKVVHVGDEVFHSRAVILATGSKY
                     RYLGLDNEARLLGHGVSACATCDGFFFRDQDIAVVGGGDSALEEATFLSKFARSVTVV
                     HRRDALRASKIMQDRAYANDKLGFRWNSEVTDVIGDEKVAGVTVRDTLTGASDQLPVT
                     GLFVAIGHDPRSELFRGQVDLDEAGYVKVEQPSTRTNLDGVFACGDLVDHTYRQAITA
                     AGTGCAAALDAERWLAAQHD"
BASE COUNT          273 a          506 c          557 g          298 t
ORIGIN      
        1 cggtgcgggg accgaagacg ccgtccgctc ggccgcaatc gaatcccatg tcggtcagtc
       61 gatgttgaag gccggcgacg tcgtcgccca cgtaggggtg ccggaccgcg aaagacaggg
      121 tgcggtcgcc tagccgccag cgggcttcgt cgaggcctcg gtaggtttca cggtcgacaa
      181 cgccgtcaat gcggaggccg cgctgctgct ggaagctgcg caccgcgtga tcgcaggctt
      241 cgtcgaacag gtcagcggct tctgagctct ggccggcacg ggtctcgtcg ttgatcaaga
      301 gaccgagctg gcgcagccga tgccgcagct cgagcacggc gagcccgcgg tcgccaaggc
      361 gcagcggctc gtcgacccgc tcgtgctgtt gctgcatggg cgtcctcgct cgtggaactc
      421 cggtgggtgt tagacgcccg gcgcggctcc ggcgtttacc ggagtcgccg ccgaggtgtg
      481 attaccggcg tggaggagcc atggtacggc ctagaggaac gcttccaggt ccttcaagag
      541 cgcggacttc gacttggcgc cgatgatctg cttgacgacc tgaccgccct tgtacacgtt
      601 cagcgtcgga atcgagagga tcgagtaggc gcgggccgtc tcagggttct cgtcgatgtt
      661 cagcttgact acccgcacct tgtcggcgtg ctcgttggcg atctcttcga gtaccggcgc
      721 gaccatgcgg cagggaccgc gccactcggc ccaaaagtcg accaggaccg gggtgtcgct
      781 gttgaggacg tcggtcgcaa aggtctcgtc ggtgacggac ttggtggctc ccacagtggg
      841 ctcctatcgg gttgaacact ctgattttca tggatggaac tggatcgatg cggtggtgag
      901 ttcagctgtc gagctggtta gtcgtgttgc gcggccagcc agcgttcggc gtcgagcgcg
      961 gcggcacaac cggtgccggc cgcggtgatg gcctggcggt aggtgtggtc gaccaggtcg
     1021 ccacaggcga agacaccgtc gaggttcgtg cgcgtcgatg gctgctcgac cttcacgtaa
     1081 ccggcctcgt cgagatcaac ctggccgcgg aacagctcgc tacgcgggtc atgaccgatc
     1141 gccacgaaca gaccggtcac cggcagttgg tcgctagcac ctgtgagggt gtcgcgcact
     1201 gtgacgccgg cgaccttttc gtcgccgatg acgtcggtga cttcagagtt ccagcggaag
     1261 ccgagcttgt cgttcgcgta cgcacggtcc tgcatgatct tgctcgcacg caacgcgtca
     1321 cgccggtgca cgacggtgac actgcgcgcg aacttgctca ggaaggtggc ttcttcgaga
     1381 gcggagtcgc cgccaccgac gaccgcgatg tcctggtcac ggaagaaaaa tccgtcacac
     1441 gtcgcacaag ccgagacacc atggccgagc agtcgagcct cgttgtcgag accgaggtag
     1501 cgatacttcg agcccgtcgc caagatcacc gcacgtgagt gaaagacctc gtcgccgacg
     1561 tgcacgacct tgatcggccc ggtcaggtcg accgccgtga cgtcatcggt caccagttcg
     1621 gcgccgaagc gagc
//
LOCUS       DASWVF010000064         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_281886, whole genome shotgun sequence.
ACCESSION   DASWVF010000064 DASWVF010000000
VERSION     DASWVF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281886"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGD55_01105"
     CDS             <1..463
                     /locus_tag="VGD55_01105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY0868969.1"
                     /translation="IGTGCGVSGVWLLRGMNPEGILTTIDVEAEHQRLAKQAYADAGF
                     ATTRTRLISGTALDVLTRLTDGAYDLVFCDGDKKEYGDYLREALRLLRVGGVVAFDNS
                     TWHHRVADPSQRDPATITIRDLGRQLREEPGLVPTLLPVGDGLLVAVKREA"
     gene            complement(460..1605)
                     /locus_tag="VGD55_01110"
     CDS             complement(460..1605)
                     /locus_tag="VGD55_01110"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0868970.1"
                     /translation="MRALAEGLLLGGYSFKVTNNPKPDVLRSVWLVVERAEARAHALH
                     EGVLTAAATMVARDLANTPSSTKTPAWLGRQAVALARTNDLDVHVRNDRELRANGFGG
                     VVAVGMGSANPPNLIELTYTPDPAIAVEGHVVLVGKGITFDSGGLSLKPNAGMIAMKT
                     DMAAGGAVIAAMSAMRDLGVRVRVTGLIPAAENMPSGTAQRPSDVITQYGGTTVEVLN
                     TDAEGRLVLADALAYADLELDPDVIVDLATLTGAISSGLGKRHAGLFTEDDRLAATLI
                     AAGNESGEALWRMPLVDDYSVVLESSVADLANVAVDGGRTFSGGAIVAALFLREFVGR
                     RRWAHLDIAGVGRAEADEHEVTKGPTGFGVRLLLHWLSSADPLRGLR"
     gene            complement(1606..1971)
                     /locus_tag="VGD55_01115"
     CDS             complement(1606..1971)
                     /locus_tag="VGD55_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0868971.1"
                     /translation="MPPTLELARSVPSGVTALVVGMTPPTEDQVDEGAVLDRGAVADL
                     EAFGVDPADLTAVLAREKVKGNVGEIASLPVGARLVFAAGLGERTPRDYRRAGAAISR
                     RLKNIDTAAITVARRSPGA"
     gene            complement(2005..2172)
                     /locus_tag="VGD55_01120"
     CDS             complement(2005..2172)
                     /locus_tag="VGD55_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEY0868972.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVELSADEAM
                     ALGDALKNVVG"
     gene            complement(2296..2880)
                     /locus_tag="VGD55_01125"
     CDS             complement(2296..2880)
                     /locus_tag="VGD55_01125"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY0868973.1"
                     /translation="MSAPVGPDGLRRCDWSYSAPEYIDYHDNEWGRPVHGDDAVYERM
                     VLEAFQSGLSFITILRKRPAFRKAFAGFSIEAVAQFTEADVDRLMSDAGIVRNGAKVN
                     AAIANARAALALPEGVSELIWSFAPTRLGRKRPAPKVQADLPAATPESTAMAKELKRR
                     GFRFIGPTTAYAHMQATGMVNDHLATCHARKLMG"
     gene            complement(2877..3362)
                     /locus_tag="VGD55_01130"
     CDS             complement(2877..3362)
                     /locus_tag="VGD55_01130"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0868974.1"
                     /translation="MAELVVSTVVNAALDKVWAALTDWDTHGDWMVATIVRGTTDQHD
                     GVGAGIEGITGIGSLVMRDTMTLSQWQPPPANPARCVVEHTGKVVRGSGAFEVEELGP
                     DQSRVVWSEWVQLPLGLLGETGWIAVRPLAALFLRISLRRLARIVEADSVTTDAEAHS
                     T"
     gene            complement(3369..3836)
                     /locus_tag="VGD55_01135"
     CDS             complement(3369..3836)
                     /locus_tag="VGD55_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY0868975.1"
                     /translation="MPLLFGILVAAVVIFAIFAVTIGHGGSMTQFRPDWPGKPLPEDR
                     TVRAHDLADAQFSLAFRGYRMAEVDEALDRLAVEIAQRDEQIERLTGQPFESLKPPEP
                     EPVTPDVVTHEPLSAEQPPVHEYAAPEYSTPTLEGSGPPTQPIPPVIEPGAER"
     gene            3925..>4444
                     /locus_tag="VGD55_01140"
     CDS             3925..>4444
                     /locus_tag="VGD55_01140"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0868976.1"
                     /translation="MPLRLGSREFADHEFVVMGIVNRTPDSFFDQGATFAIDAALRAV
                     DVAVADGAAIVDIGGVRAGPGPDVDTAEEIRRVAHFVALVREKHPSLVISVDTWRAEV
                     AHAVVEAGADLLNDAWGGHDPDLAKVAASAGIGLVCSHAGGLPPRTDPHRVAYDDVVR
                     DVVETVTRLADRA"
BASE COUNT          724 a         1491 c         1492 g          737 t
ORIGIN      
        1 gatcggcacg gggtgcggag tctcgggcgt ctggttgctc aggggcatga accccgaagg
       61 catcttgacc acgatcgatg tcgaagctga gcaccaacga ctcgcgaagc aggcctacgc
      121 cgacgccggt tttgcgacga cccgcacgcg tctgatctcc ggcacggcac tcgacgttct
      181 cacgcggctg actgacggtg catacgacct ggtcttctgc gacggcgaca agaaggagta
      241 cggcgactac ctgcgcgagg cactacgact gctgcgcgtc ggcggggtcg tagcgttcga
      301 caactcgacc tggcatcacc gagtggccga tccctcgcag cgcgacccgg cgacgatcac
      361 gatccgcgat ctgggccggc aactgcgcga agagccagga ttggtgccga cgctgctgcc
      421 cgtgggtgac ggtcttctag tagcggtcaa gcgcgaggct tagcgaaggc cgcgcagcgg
      481 gtcggccgaa ctgagccagt gcagcaggag gcggacgccg aacccggtcg gtcccttcgt
      541 cacttcatgc tcgtctgctt ctgcccgacc aacgccggcg atgtcgagat gcgcccaccg
      601 gcggcgtccg acaaattctc tgaggaacag cgccgcgacg atcgcgccgc cgctgaacgt
      661 acggccaccg tcgacggcga cgtttgcgag gtcggctacc gacgactcga gcacgacgct
      721 gtagtcgtcg acgagcggca tccgccacag ggcctcgccg ctctcgttgc ccgcggcgat
      781 gagagtggcg gcgagtcgat cgtcttcggt gaacaggccg gcatggcgct tgccgagccc
      841 actggaaatc gccccggtca acgtcgcgag gtcgacgatc acatcagggt cgagttcgag
      901 gtcggcgtac gcgagcgcat cggcaagcac caaccttccc tcggcgtcgg tgttcagtac
      961 ctcgacggtg gtgccgccgt actgcgtgat gacgtcgctc gggcgttgcg cagtacccga
     1021 tggcatgttc tcggcagccg ggatcaggcc ggtcacgcgc acccgcacgc cgaggtcgcg
     1081 catcgccgac atcgccgcaa tgaccgcgcc acccgcggcc atgtcggtct tcatcgcgat
     1141 catcccggca ttgggcttca acgaaagccc gccggagtcg aaggtgatgc ccttgccgac
     1201 cagcacgacg tggccctcga ccgcgatggc ggggtcgggc gtgtacgtca actcgatcag
     1261 gtttggcgga tttgcagaac ccatgcccac ggcaacgacg ccgccgaatc cgttggctcg
     1321 caactcgcga tcgttgcgca cgtggacgtc gaggtcgttc gtacgcgcga gcgcgacggc
     1381 ttgccggccc agccacgccg gggtcttcgt cgacgacggc gtgttcgcga gatcgcgcgc
     1441 caccatggtc gcggcggcgg tcagcacgcc ttcgtgcagg gcatgagcac gcgcttctgc
     1501 gcgctcgaca accagccata ccgaccgcaa aacatcgggc ttggggttgt tggtcacctt
     1561 gaacgaatag cccccgagca gcaggccctc ggcgagtgcg cgcacctaag ccccgggcga
     1621 gcgccgcgca actgtaatcg ccgccgtgtc gatgttcttc agtcgccgtg agatcgcagc
     1681 tccggcgcga cggtaatcac gcggcgtccg ctcaccaagg cctgccgcga agaccaaccg
     1741 cgcgccgacc ggcaatgatg cgatttcacc gacgttgccc ttgaccttct cgcgcgccaa
     1801 gaccgcggtc agatctgcgg ggtcaacacc gaacgcctcg aggtcagcga ccgcgccgcg
     1861 gtcgagtacc gcgccttcgt cgacttggtc ttcggtgggc ggcgtcatgc caacgaccag
     1921 tgcggtgaca ccggagggaa cggagcgggc aagctctaac gtgggcggca caaggtcgac
     1981 gttacccctg gcccgcagcc ccgatcagcc gaccacgttc ttgagggcgt cgcccagggc
     2041 catggcctcg tcggcggaca actcgacaac cagccggcca ccgccttcga gagggacgcg
     2101 catcacgatg ccccgcccct ccttggtgac ctcgagcgga ccgtcgcccg tccgcggctt
     2161 catggccgcc atgcgcgctc cctttccatg tccggcacgg cagtgccgga ctcccatccg
     2221 ctgtcttgac caagattatc gccgtaggga ggcagcagaa ggaaaccggc gcccctgtga
     2281 gacctcgctg cgggcctacc ccatgagctt gcgggcgtgg caggtggcca gatggtcgtt
     2341 gaccatcccg gtcgcctgca tgtgcgcgta cgcggtcgtc ggcccgatga atcgaaaccc
     2401 gcgccgcttc agttccttgg ccatcgcggt cgactctggg gtcgctgcgg gtaggtcggc
     2461 ttgaactttg ggggccggcc tcttgcgtcc gagacgcgtg ggcgcgaacg accagatgag
     2521 ctccgacaca ccctcaggta gggcaagagc ggcccgtgcg tttgcgatcg cggcgttgac
     2581 cttggcgccg tttcgcacga tgcccgcgtc ggacatcagc cggtcgacgt ccgcctcggt
     2641 gaactgcgcg acggcttcga tcgagaagcc ggcgaatgcc ttccggaacg ctggtcgttt
     2701 ccgcaggatc gtgatgaacg agagtccgga ctggaacgcc tcgagcacca tgcgctcgta
     2761 gaccgcgtcg tcgccgtgga ccggccgacc ccattcgttg tcgtggtagt cgatgtactc
     2821 gggcgcgctg taggaccagt cgcatcgacg caaaccgtcg gggccgaccg gtgcgctcat
     2881 gttgagtggg cctcggcatc ggttgtcacg ctgtcggctt cgacgatgcg tgcaagccgt
     2941 cgcagcgaga tccgcagaaa gagagcagcc aacggccgga cggcgatcca gccggtctca
     3001 ccgagcagac cgagcgggag ttgcacccac tccgaccaca ccacccgcga ctggtcgggg
     3061 ccgagttctt ccacctcgaa cgcacccgac ccccgcacca ccttgccggt gtgctcgaca
     3121 acacagcgcg ccggatttgc cggcggcggc tgccactgcg acaacgtcat cgtgtcgcgc
     3181 atgaccaagg agccgatccc ggtgatgcct tcgatgcccg cgccgacgcc gtcgtgctgg
     3241 tcggtcgtgc cgcgcacgat ggtcgcgacc atccagtcgc cgtgggtgtc ccagtcggtc
     3301 agcgccgccc acaccttgtc gagtgccgcg ttcacaacgg tcgatacgac cagttcagcc
     3361 acggtgggct atcgctccgc cccgggttcg atcaccggag ggatcggctg agtcggcggg
     3421 ccactgcctt ccagcgtggg cgtgctgtac tcgggcgcgg cgtactcatg cacgggaggc
     3481 tgctcggcgc tcagtggctc gtgcgtgacc acgtcgggcg tgaccggctc gggctctggt
     3541 ggcttcagcg attcgaatgg ctggccggtc aagcgctcaa tctgctcgtc gcgttgcgcg
     3601 atctcgaccg cgagccggtc gagggcctcg tcgacctccg ccatgcggta tccgcgaaac
     3661 gcgagactga actgcgcgtc ggcgagatcg tgcgcgcgga ccgtgcgatc ttcgggcagc
     3721 ggcttgcccg gccagtcggg tcggaattgg gtcatcgatc cgccgtgacc gatcgtcacc
     3781 gcgaagatgg cgaagatgac gacggcggca acgagaatcc cgaagaggag gggcaccccc
     3841 cgatcgtgcc atgccaacct gggcggagga tggcaggcac cccgagttcc gctacctaag
     3901 cccgaaccgt tgatgggaga gccgatgccg ctgcgcctcg gttcgcgcga gttcgccgac
     3961 cacgagtttg tggtcatggg catcgtcaac cggacgcccg actcgttttt cgaccagggt
     4021 gcgacgtttg cgatcgacgc ggcgctgcgg gcggtcgacg tcgcggtcgc tgacggagca
     4081 gccatcgtcg acatcggcgg cgtgagagca ggccctgggc cggacgtcga tactgccgag
     4141 gagatccgca gggtcgccca ctttgtcgct ctggtaagag aaaagcaccc gtcgctcgtc
     4201 atcagcgtcg acacctggcg cgctgaggtg gcccacgcgg tggtcgaagc gggggccgac
     4261 ctgctcaatg acgcgtgggg tggccatgat ccggatttgg ccaaggtcgc ggcgtccgct
     4321 ggtataggtc ttgtgtgttc gcatgcaggc gggctgccac cgcgcaccga cccgcatcgc
     4381 gtggcctacg acgacgttgt gagggatgtc gtcgagacgg tgaccaggct cgcagatcgc
     4441 gcgg
//
LOCUS       DASWVF010000065         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_154565, whole genome shotgun sequence.
ACCESSION   DASWVF010000065 DASWVF010000000
VERSION     DASWVF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154565"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="VGD55_01145"
     CDS             complement(<1..1507)
                     /locus_tag="VGD55_01145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0868977.1"
                     /translation="MLAPRLAETPRVSADPHDRLDRAGVAASPDHADGLPRPLRPVSN
                     GPAARTHWQRRYALALVTLDAILLFAGTTLAALLRWGAPAGSIKVGGLDYYAIAALTA
                     VVWLVMLASTHSYDARYLGIGSDEFRRVADAAIRVTALVAFVTFVGKLPLSRGFVAMA
                     LPIGLASTLTGRYAARKLLHHLRRKAQCLHRVLVVGTPSAASKLAEGLSRSVYTGYHV
                     CGTWSPSAADFAGAEQATRERSKVLDVLARTQADTVAVASSGFSPAALRQLAWQLEGS
                     GVDLLVSPALTDVAGPRIHMRPVDGLPLLHVEEPQLDGGKKLAKSGFDRVAALLLLIV
                     AAPVLVGLMVAIRVTSPGPAIFRQRRAGRGGAPFTIYKFRTMHQGAEATFDELVAAQV
                     DKPRGMFAKDFADERVTSVGRFLRRYSLDELPQLFNVLLGQMSLVGPRPLPATVDQVG
                     TDVSRRLLVAPGMTGLWQVSGRSDLPWDESIRLDLYYVENWSPAFDLMILWK"
     gene            complement(1559..2419)
                     /locus_tag="VGD55_01150"
     CDS             complement(1559..2419)
                     /locus_tag="VGD55_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY0868978.1"
                     /translation="MPELPEVEALATFLVTHAVGHRIDRVDVGSFQVLKTFDPPASAL
                     QGTTITGASRRGKFLDIETTGPHLVVHLARAGWLRWRQELAPAPIRPGKSPIALRVKL
                     DDGSGFDLTEAGTQKRLAAYVVVDPATIEGVARLGPEPLDSAFDVEAFATLLRGRRTQ
                     IKGLLRDQSVIAGIGNAYSDEILHVAKMSPFKIADSLTADEIGVLYEAMQTTLRDAVK
                     RAADLDAGELKAEKKANLRVHGRTGQPCPVCGDTVREVSFADSSLQYCATCQTGGKPL
                     ADRRLSRLLK"
     gene            complement(2605..>3046)
                     /locus_tag="VGD55_01155"
     CDS             complement(2605..>3046)
                     /locus_tag="VGD55_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868979.1"
                     /translation="IPPRLASGAFILNAGIGKLSAEADAAERVHTMAAGTYPFLAKVD
                     PRVFVKALAVGEIGVASVLLAPVVPAWLAGAVLTGFSGGLLAMYLKTPDMTRDDGIRP
                     TPKGTPLAKDVWMLGSGLNLFLDGLADRRRKAARAKTRAAARAT"
BASE COUNT          530 a         1019 c         1035 g          462 t
ORIGIN      
        1 tcttccacaa aatcatcaga tcgaacgcgg gcgaccagtt ctcgacgtag tagaggtcga
       61 gccggatcga ttcgtcccac gggagatcgg agcggccgct gacttgccaa aggcccgtca
      121 tgccgggggc gacaagcagc cgccggctca cgtcggtgcc gacctgatcg acggtcgcag
      181 gcaacggccg cggaccgacc agtgacatct gaccaagcag cacgttgaac agttgcggca
      241 actcgtcgag tgagtagcgc cgcaggaacc gcccgaccga cgtcacacgt tcgtcggcaa
      301 aatcctttgc gaacatgcca cgcggcttgt cgacttgagc ggcaacaagc tcgtcgaacg
      361 tcgcttcggc accctgatgc atcgtgcgga acttgtagat cgtgaagggc gcaccgccgc
      421 gcccagcgcg tcgctgcctg aagatggcgg ggccaggact cgtcaccctg atggcgacca
      481 tcaaaccgac aagcacaggc gccgcgacga tcaaaagcag caacgcggcc acgcggtcga
      541 accccgactt ggccagcttc ttcccaccgt cgagttgcgg ctcttcgacg tgcagtagcg
      601 gaaggccgtc gaccggacgc atgtgaatcc gcggaccggc gacatcggtc aacgcgggcg
      661 agacgagaag gtcgacaccg ctgccctcga gttgccaggc aagttggcgc aaggcggctg
      721 gactgaagcc agacgatgcg accgccaccg tgtcggcctg ggtccgggcg agcacgtcga
      781 gcaccttgct acgctcgcga gtcgcttgct ccgcaccggc gaagtcggcg gcggacggcg
      841 accaggtccc gcatacgtga tagccggtgt acaccgagcg gctgaggccc tcggcgagct
      901 tgctcgctgc cgacggcgtg cccacgacca ggacccggtg caagcactgt gctttgcgcc
      961 gcaggtgatg cagcagtttg cgcgcggcat aacgcccggt gagagtcgat gcgagtccga
     1021 tcggcagtgc catcgcgaca aacccgcgcg agagcgggag cttgccgacg aaggtcacga
     1081 aggcgacgag tgctgtgacc cggatcgcgg cgtcggcgac ccgacggaat tcatcagagc
     1141 cgatgccgag gtacctggcg tcatacgagt gagtcgaagc cagcatgacc agccacacca
     1201 cggcggtgag ggcggcgatc gcgtagtagt cgaggccgcc gaccttgatg ctgcctgcgg
     1261 gggcgcccca cctcagcagc gctgcgagcg tcgttcccgc gaagagcaag atcgcgtcca
     1321 aagtgacgag tgcgagtgcg tagcgccgct gccaatgggt gcgcgctgca ggcccgttcg
     1381 acactggccg cagcggccgc ggcaggccgt cagcgtgatc ggggctggcg gcgacgccgg
     1441 cgcggtcgag gcggtcgtgc ggatctgcac tcacccttgg tgtctcggcg agtcgcgggg
     1501 cgagcatgag ggcctgacgg gcgcgatata cgcccgttcg gcctaatccg gcacggcgct
     1561 acttcagcag gcgcgagagc cggcggtcgg cgagcggctt gccgcccgtc tgacaggtcg
     1621 cgcagtactg gagcgacgag tcggcgaagg acacctcccg cactgtgtca ccgcacaccg
     1681 ggcacggctg gccggtccgg ccgtgcacgc ggaggttggc tttcttctcg gctttcagtt
     1741 cgccggcgtc gaggtcagcc gcccgcttta cggcgtcccg caaggtcgtt tgcatggcct
     1801 cgtagagaac gcctatctca tcagcagtca ggctgtcggc gatcttgaag ggcgacatct
     1861 tggcaacgtg caggatctcg tcgctgtacg cattgccgat acccgcgatg accgactgat
     1921 cgcgcagcaa ccccttgatc tgggtgcgcc tgccccgcag cagggtcgcg aacgcctcga
     1981 cgtcgaacgc cgaatcaagc ggctccgggc caagccttgc cacgccctcg atcgtggctg
     2041 ggtcgacgac gacgtacgcc gcgagccgct tctgggtgcc cgcctctgtc agatcgaagc
     2101 ccgaaccgtc gtcaagtttc acccgcaatg cgatggggct cttgcctggc cggatcggcg
     2161 caggtgcaag ctcctgccgc catcgcaacc aaccagcacg agcaaggtgg acgacgaggt
     2221 gtggcccagt tgtctcgatg tcgagaaact tcccccgccg gctggcgccc gtgatcgtag
     2281 tgccctgcaa cgctgacgcc ggcgggtcga atgtcttcag aacctggaac gagcccacgt
     2341 cgactcggtc gatgcggtga ccgacggcgt gggtgaccag aaaggtcgcg agcgcctcga
     2401 cctcgggcag ttcaggcacg acgtcgatga tgccgcatta ccaagcccgt gacccgccgc
     2461 cccgaccgcc gtgcgatgac cacgttcagg gggttgcgcc ccccgaggac acgcttgcaa
     2521 cggtgtcttc gggggccggg gcctgtcctc ggccgggtag ggctcgagat gatcttggac
     2581 ggcgggatgg tggccgccgc gccgtcaggt ggcccgagcc gcggcgcggg tcttggcccg
     2641 cgcggccttg cgccgccgat ccgccaagcc gtcgaggaag aggttcaggc cggagcccag
     2701 catccagacg tccttcgcga gcggcgtgcc cttgggagtc ggtcggatgc cgtcgtccct
     2761 cgtcatgtcc ggcgtcttga ggtacatcgc cagcaacccg ccggaaaagc cggtgagcac
     2821 cgcgccggcc agccatgccg gaacgaccgg tgcgagcagc accgacgcga cgccgatctc
     2881 cccaaccgcg agagccttca cgaagacccg cgggtcgacc ttcgccagga acgggtacgt
     2941 gcccgcggcc atggtgtgca cccgctccgc ggcgtccgcc tcggcagaca gcttgccgat
     3001 gccggcattc aagatgaacg ccccgctcgc gagacgcggg ggtatg
//
LOCUS       DASWVF010000066         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_460314, whole genome shotgun sequence.
ACCESSION   DASWVF010000066 DASWVF010000000
VERSION     DASWVF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460314"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VGD55_01160"
     CDS             complement(<1..1165)
                     /locus_tag="VGD55_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0868980.1"
                     /translation="MTSRFRTRLRTVIALMATVLAPAAYGEGSSPAAAAAPAISSLTP
                     PKLLTFVDAAYPVAAKAAGRQANVDLEITIDATAKVTDVRVVAPAGDGFDEAAVEAAR
                     RFVFAPARRGDRAIPARIRYRYVFELAAEAVPAPTTGELEGRVLARGQERVIEGARVT
                     LFSDDGQIARTAVTNALGAFHFQALPPGRFHLALAAAARAALVVEEDVAAGELTSVTY
                     RMDASRPSGDGPTLEFGATAIIDAPPREVTKRSLSGEELVRMAGTRGDPLRAIEYMPG
                     VGRSPQAELIIIRGSSPADSEVQFEGAPVYRLYHFGGLTSFVQPRLLAKIDLYPGNFS
                     ARFGRKMGGIIDVGVRDPRTDGFHGLVDVNVVDSSFLFEGPLTANWSFAVAAKR"
     gene            1318..2496
                     /locus_tag="VGD55_01165"
     CDS             1318..2496
                     /locus_tag="VGD55_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0868981.1"
                     /translation="MAHISPVALLVESLMLAGASVFGVWASRSRAQARARAEALDTQL
                     EQQRTAGERREGVLRTVVETTPVAMVLFGDAGNIIFTNRSARDLFFDGQAVEGQNFLS
                     MLERAPVSLRQALVSEGDELVSVQGPAERETFHLSRRRLDAGQTLIAVRSVTQEINRH
                     EVASLKKVIRIISHEINNSLGPIASLVSSAKLILQRPEHLPKLPSMFDTIQERTLHLR
                     DFLDGYAKLAKIPQPQRASTAWRPCLDGLREFWPNLQITDTTVRLGFFDRAQIQQVLI
                     NLIKNAHEVGGPESEVKVVAESVADGGCRISVLDRGPGVSEEVMRNVFVPFFTTKPTG
                     SGLGLALSREIVELHQGRLGLARREGGGMSVSVWLPDTDGASVGALAASRARLTLTRT
                     "
     gene            2683..3300
                     /locus_tag="VGD55_01170"
     CDS             2683..3300
                     /locus_tag="VGD55_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868982.1"
                     /translation="MKLATFSVSTFFLLTSAMALAQSPSPASSEPETLTAGTSPGGTK
                     ITGWFLAPTFGTTGFDGRLNYTPGVRGGIYLNKRFAVGIAAQGLASSASTLNHDEVRN
                     LGSYGGLLLQYIWHSDQLFHGTLESTIGNGRWCAAGTGGSDSCATKGFLVFEPAANIE
                     LNVAKHVRFASGVGYRFAAAGSGEGPSSHDMSSLVVRTSLIFGSF"
     gene            3410..3808
                     /locus_tag="VGD55_01175"
     CDS             3410..3808
                     /locus_tag="VGD55_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY0868983.1"
                     /translation="MDETVQRVVDLLQAKGATLFALIDHSGEASRAGLHMRATKLVVF
                     GSPRAGTPLMQAAPRAAIDLPLKILVWQDDDGATRTVPSGCESATAWTQLPWPSWRPR
                     TPSPRRSQGRKQAARGAHPRNCVCHPYANR"
     gene            complement(3821..>4534)
                     /locus_tag="VGD55_01180"
     CDS             complement(3821..>4534)
                     /locus_tag="VGD55_01180"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY0868984.1"
                     /translation="GGPGGFERFEISDVSGDMSFLEMLDVLNERLIASGTQPVAFESD
                     CREGICGACGFMINGDAHGPLPGTTVCQLHMRSFHDGQELWLEPWRARAFPVVRDLIV
                     DRAALDRIIASGGYVSAPTGSAVEANTIPIRKSDADAAMDAAACIGCGACAAACPNGS
                     AALFTAAKIAHLGVLPQGQPERHARAISMVTQADAEGFGGCSNVGACQEACPKSIPAG
                     LIRRMNRDVLVGSLRRHRD"
BASE COUNT          767 a         1524 c         1517 g          726 t
ORIGIN      
        1 tccgtttggc ggcgacggcg aaggaccagt tcgcggtgag tggcccttcg aagaggaacg
       61 aactgtcgac gacgttgacg tcgaccaacc cgtggaagcc gtccgtcctg gggtctcgga
      121 caccgacgtc gatgatgccg cccatcttgc gtccgaagcg cgccgagaag ttgccggggt
      181 agaggtcgat cttcgccagc agccgcggtt gcacgaagct ggtcaggccg ccgaagtggt
      241 aaaggcgata gacgggcgcg ccctcgaact gcacctcgga gtctgcgggc gacgagcccc
      301 ggatgatgat gagctcggct tgcggcgaac gcccgacgcc cggcatgtac tcgatcgccc
      361 tcaaggggtc gccgcgcgtg cccgccatgc gcacgagctc ctctccgctg aggcttcgtt
      421 tcgtgacctc gcgggggggc gcgtcgatga tcgccgtcgc gccgaattcg agagtcggcc
      481 cgtcgcccga gggacggctc gcgtccatgc ggtaggtgac cgaagtcagc tcgcccgcgg
      541 cgacgtcctc ttccacaacg agcgcggccc gcgcggccgc ggccagcgcg aggtgaaacc
      601 gccccggggg aagggcctgg aaatggaacg ctcccaacgc gttggtgacc gccgtgcggg
      661 caatctgacc atcgtcggag aagagagtga ccctcgctcc ctcgatcacc cgctcctgac
      721 cgcgcgccag cacgcgacct tcgagctcgc ccgtcgtcgg cgccggcacg gcttccgcag
      781 ccagctcgaa gacgtaccgg tacctgattc gggctgggat cgcgcgatct ccgcgacggg
      841 ccggcgcgaa tacgaacctg cgggcggcct cgactgcggc ctcgtcaaat ccatcgcctg
      901 ccggcgcgac gacgcggacg tcagtgacct tcgccgtggc gtcgatcgtg atctcgagat
      961 cgacgttcgc ttgccggcct gctgccttgg cggcgacggg ataggccgcg tcgacgaacg
     1021 tcagcagctt gggtggcgtc agcgagctga tcgcaggtgc cgccgcagcc gcgggcgagg
     1081 agccctcgcc gtatgctgcg ggggcgagca ccgtcgccat cagagcgatg acggtgcgaa
     1141 gacgtgtccg gaagcgagag gtcatggcgg gctccttgtg cagccgccgt gccggcaatc
     1201 ccgcagggac gtcgcgcctt tttgcacgtg ccggaacacc tgtccgccgg acgattgccc
     1261 gcaggtgtcc gctcacgacg ttggtgatcg cgcgatcgat cggataccgt cgcgaccttg
     1321 gcgcacatct cacctgtcgc gttgctcgtc gagagcctca tgctggcggg tgcgtcggta
     1381 ttcggcgtct gggccagtcg gtcgcgcgcc caggcccggg cgcgtgccga agcgttggac
     1441 acgcagctgg aacagcagcg gaccgccggt gagcgccgcg agggcgtgct ccgaacggtc
     1501 gtggagacga ccccggtggc gatggtgctg ttcggcgacg ccgggaacat catcttcacg
     1561 aatcggtcag cgcgcgacct gttcttcgat gggcaagccg tcgagggaca gaacttcctc
     1621 tcgatgctgg aacgagcccc cgtgtcgctg cgtcaggccc ttgtatccga gggtgacgag
     1681 ctcgtcagcg tccaggggcc ggcggaacgc gagactttcc acctttcgcg gcggcggcta
     1741 gatgccgggc agacgttgat cgcggtgaga agcgtcaccc aggagatcaa ccgtcacgaa
     1801 gtcgccagcc tgaagaaggt catccgcatc atcagtcacg agatcaacaa ctcgcttggc
     1861 ccgatcgctt cgctcgtcag ctccgcgaag ctgatcctgc aacgccccga gcacctgccc
     1921 aagctcccgt ccatgttcga cacgattcag gagcgcacgc tgcacctccg agacttcctc
     1981 gacgggtacg ccaagctcgc gaagatccct cagccacaac gcgcgtcgac agcatggcgt
     2041 ccctgcctgg acgggctgcg cgagttctgg ccgaatctac agatcaccga tacgacggtc
     2101 agactcggct tcttcgatcg ggcgcagatt cagcaggttc tcatcaacct gataaagaac
     2161 gcgcacgaag tcggaggacc agaaagcgag gtcaaggtcg tcgccgagag cgtggcagac
     2221 ggcggatgcc ggatctcggt gcttgatcgc ggccccggcg tctccgagga ggtgatgcgg
     2281 aacgttttcg ttcccttctt cacgaccaaa ccaaccggca gcggcctcgg gctggcgctc
     2341 tcccgcgaaa tcgtcgagct gcaccaggga cgcctggggc tggcccgtcg cgaaggcggc
     2401 ggcatgtccg tcagcgtctg gttgcccgat acggacgggg cgtccgtcgg cgcgctggcg
     2461 gcgagccggg ctcggctcac gttgacccgt acgtagcgct cacaccgccg cgcaatgtcg
     2521 cgctcggacg gttggcggac aggtgtcccg tccacgcgtg acagatgtcc gggctatcgg
     2581 cggcgcagcc ttgaatacgc gcaggcgaac gcggagtcga gatggcatgc gccgtgctgt
     2641 taccggtcgc gagctgttca acacacaccc aggagccaca tcatgaagct tgcgaccttt
     2701 tccgtttcga ccttcttcct gctgacgagc gcgatggcgc tggcgcaaag tccgtcgccc
     2761 gcgtcgtcag agccggaaac cttgaccgcg ggaacgtctc ccggcggaac gaagatcacc
     2821 gggtggttcc tggcgccgac gtttggcacc acgggattcg acggccgtct gaactacacg
     2881 cccggagtgc gcgggggcat ctacctgaac aagcgcttcg ccgtcggcat cgccgcccag
     2941 ggactggcga gctccgccag cacgttgaac cacgacgagg ttcggaacct cggaagctac
     3001 ggggggctgc tgctgcagta catctggcac tcggaccagc tattccacgg cacgctggag
     3061 tcgacgatcg gaaacggccg ctggtgcgcc gccgggacgg gcggctccga ctcgtgcgcg
     3121 acgaaggggt tcctggtctt cgaaccggcc gcaaacatcg agctcaacgt tgccaagcac
     3181 gtgcgattcg ccagcggagt gggctaccgg ttcgccgcgg ccggaagcgg cgaaggtccg
     3241 tcgagccacg acatgagcag cctggtcgtg cgcaccagcc tgatcttcgg cagcttctga
     3301 ccgctcaccg tggcaagctc gtcgtcgtga acgaaccatg caaccgaggg caggcgccgt
     3361 gacagttcct gacggcgccg gtctgatcga tcacctctgt cctcgttcgg tggacgaaac
     3421 ggtccaacgc gtcgtcgacc tgctgcaagc aaagggcgcc acgctgttcg cgctcatcga
     3481 tcacagcggc gaggcatcga gagccggtct gcacatgcgc gcgaccaagc tggttgtctt
     3541 cggcagtccc cgcgccggca ccccgctcat gcaggcggcg ccgcgggctg ccatcgactt
     3601 gccactgaag atcctggtct ggcaagacga cgacggtgcc acgcgaacag tcccgagtgg
     3661 ctgcgagagc gccacggcct ggacgcagct gccctggcca tcctggcggc cgcggacgcc
     3721 atcgccgcgg cggtcgcagg gacgtaaaca ggcagcgcgc ggcgcccacc ctcgcaattg
     3781 cgtctgccac ccctacgcaa atcggtgagc gggcgcaaac tcagtcgcga tgacgtcgca
     3841 ggctgccgac caggacgtcg cggttcattc gacggatcaa accggcgggg atggacttcg
     3901 gacatgcctc ctggcacgcg ccgacgttgc tgcaaccccc aaatccctcc gcgtccgcct
     3961 gggtcaccat cgagatggcc cgggcgtggc gttccggttg gccctgcggc aacacgccga
     4021 ggtgggcgat cttggcagcc gtgaacaggg ctgctgatcc attcgggcag gcggcggcgc
     4081 atgcgccgca cccgatgcac gccgccgcat ccatcgccgc atcggcatcg gatttccgga
     4141 tcggaatcgt gtttgcttcg actgcgctgc cggtcggcgc gctgacgtac ccgcccgaag
     4201 cgatgatccg atcgagggcg gcgcggtcca cgatcaggtc gcgtaccacg ggaaaggcgc
     4261 gcgcgcgcca gggctcgagc cagagctctt ggccgtcgtg aaagctgcgc atgtgaagct
     4321 gacacaccgt cgtacccggc agcggcccat gcgcgtcgcc gttgatcatg aacccgcagg
     4381 cgccgcagat gccctcacgg caatcgctct cgaaggcgac gggctgggtg ccagaggcga
     4441 tgagccgttc gttcagcacg tccagcatct ccaggaacga catgtcgccg gatacgtccg
     4501 aaatctcgaa ccgctcgaac ccgcctgggc cacc
//
LOCUS       DASWVF010000067         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_52638, whole genome shotgun sequence.
ACCESSION   DASWVF010000067 DASWVF010000000
VERSION     DASWVF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52638"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGD55_01185"
     CDS             complement(<1..1049)
                     /locus_tag="VGD55_01185"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0868985.1"
                     /translation="MSPSTDRTALEELLREWLPKQRWFAGKDRGAKAVHITRERELLA
                     GDPALVHMLAEVEQLPQLDRLDKPANDKLTTMYQLVLGVRAELPHELEHASIGYVGEL
                     GQVYDASQDRELMHVVLGWVASDETRHGIVFASEPGVVIDRDATSLVLGGEQSNTSIV
                     FGEQSILKLFRIVVPGLNPDLEVTRALTEMGNPHVPAIQGWIESDVEQTATTLGLLQE
                     FLRTGSDGWALALTSVRDLFAEGDLHADEVGGDFAAESERLGMATADVHSAMARALPT
                     AIADVQTLQTLARSMRERLEAARLAVPELADHAAAILAIFDALEHSDEPVALQRVHGD
                     FHLGQVMRTDNGWVLL"
     gene            complement(1051..>1992)
                     /locus_tag="VGD55_01190"
     CDS             complement(1051..>1992)
                     /locus_tag="VGD55_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0868986.1"
                     /translation="PDRVLLAEANQWPADVVEYFGEGDECHMAFHFPLMPRIFMAVRR
                     EQRYPISEILAQTPAIPDNCQWGIFLRNHDELTLEMVTDEERDYMYTEYAQDPRMKAN
                     IGIRRRLAPLLENSRDQMELFTALLLSLPGSPVMYYGDEIGMGDNIWLGDRDGVRTPM
                     QWTPDRNAGFSTCDPGRLYLPVIMDPIYGYQGLNVEAQMRTSTSLLHWTRRMIETRKR
                     HPTFGAGTFTELGASNPSILAFVREFGDDRVLCVNNLSRFPQPVELDLRRYADVVPVE
                     MLGGMHFPKVGELPYLLTLPGHGFYWFTLPAKRAEDT"
BASE COUNT          326 a          640 c          673 g          353 t
ORIGIN      
        1 agcagcaccc agccgttgtc ggtccgcatg acttgaccga gatggaagtc accgtgcacc
       61 cgctgaagag ccaccggctc gtcgctgtgt tccagagcgt cgaagatcgc cagaattgcg
      121 gccgcgtgat cagccaattc ggggacggcg agcctggccg cctcgagtcg ctcgcgcatc
      181 gaccgggcca gcgtttgcag agtctggacg tcggcgatcg cggtcggaag cgcccgcgcc
      241 atggcggaat gcacgtcggc tgtcgccatg ccgaggcgct cggactccgc ggcgaagtcg
      301 ccacctacct catcggcgtg caggtctccc tcggcgaaca ggtcgcgcac gctcgtcagc
      361 gcgagcgccc acccgtcgga gccggtgcgc aggaactcct gcaacaaccc gagcgtggtc
      421 gcggtctgct cgacgtcgct ctcgatccac ccctggatcg cgggaacgtg cgggttgccc
      481 atctccgtaa gcgctcgggt gacctcgagg tcggggttca ggccgggcac cacgatgcgg
      541 aacaacttca agatggattg ctcaccgaag acgatcgacg tgttcgactg ctcaccgccg
      601 agaacgaggc tcgtcgcgtc gcggtcgatc accacgcctg gctcgctcgc gaacacgatg
      661 ccgtgtcgcg tttcgtcgct cgcgacccag ccgagcacca cgtgcatgag ctcgcggtct
      721 tggctggcgt cgtacacctg cccgagctcc ccgacgtatc cgatgctcgc gtgctcgagc
      781 tcgtgcggca attctgcgcg gacgccgagc acgagttggt acatcgtggt gagcttgtcg
      841 tttgccggct tatcgagacg gtcgagctgc gggagttgtt cgacctcggc gagcatgtgc
      901 accagcgccg gatcgccggc gagcagttcg cgctccctgg tgatgtggac cgccttcgcg
      961 ccgcgatcct tgcccgcgaa ccagcgctgc ttcggcagcc attcgcgaag cagttcttcg
     1021 agcgccgtgc ggtcggtcga ggggctcatg tcaggtgtcc tccgcgcgct tggccggcag
     1081 cgtgaaccag taaaagccgt gccctggcag ggtcagcagg taagggagtt cgccgacctt
     1141 cgggaagtgc atacccccga gcatctcgac cggaaccacg tcagcgtacc ggcgcagatc
     1201 gagctccacc ggctgcggga agcgtgacag gttgttcacg cagagcaccc ggtcgtcgcc
     1261 gaactccctg acgaacgcca ggatgctcgg attcgacgcg cccagttcgg tgaaagtgcc
     1321 cgcaccgaag gtggggtgcc gcttgcgggt ctcgatcatc cgtcgcgtcc agtgcagaag
     1381 ggacgtcgag gtgcgcatct gggcttcgac gttcaggccc tggtagccat agatcgggtc
     1441 catgatcacc ggcaagtaga gccggcccgg gtcgcaggtc gagaagccgg cgttgcggtc
     1501 gggcgtccac tgcatcgggg tccggacgcc gtcgcgatct ccgagccaga tgttgtcgcc
     1561 catgccgatc tcgtcgccgt agtacatgac cggtgagccc ggcagtgaca gcagcaaggc
     1621 tgtgaagagc tccatctggt cgcgggagtt ctccaggagc ggagccaacc ggcggcggat
     1681 gccgatgttg gctttcatcc gcggatcctg ggcgtactcg gtgtacatgt agtcgcgttc
     1741 ctcgtcggtc accatctcaa gggtgagctc gtcgtgattg cgcaggaaga tgccccactg
     1801 gcagttgtcc gggatggctg gggtctgcgc caggatctcc gagatcgggt aacgctgctc
     1861 gcggcgtacg gccatgaaaa tgcgtggcat caaagggaag tggaaggcca tgtggcactc
     1921 gtcgccctcg ccgaagtact cgacgacgtc ggccggccac tggttggcct cggcgaggag
     1981 cacgcggtcc gg
//
LOCUS       DASWVF010000068         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_485767, whole genome shotgun sequence.
ACCESSION   DASWVF010000068 DASWVF010000000
VERSION     DASWVF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485767"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..661)
                     /locus_tag="VGD55_01195"
     CDS             complement(245..661)
                     /locus_tag="VGD55_01195"
                     /inference="COORDINATES: protein motif:HMM:NF022668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HEY0868987.1"
                     /translation="MTPAEAATAPDLVLVKTDSEAVIEADGATVVVEAEGALAAFDPA
                     VAVEPHVRKSGVFGELPLALVIGGVGAGLVVIALHHFRWGNLLIAGSLLAGALFRSVL
                     PTRRAGLLAVRARFTDVVTMAVMGAGLLVLALVTST"
     gene            complement(686..1576)
                     /locus_tag="VGD55_01200"
     CDS             complement(686..1576)
                     /locus_tag="VGD55_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019261778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEY0868988.1"
                     /translation="MTAIILDGKATAAAIKADLKIRVASLRDRGMAPGLGTVLVGDDP
                     ASHAYVAGKHRDCAEVGIASIERNLPASASQAEVEAVVRELNADPRCTGYIVQLPLPR
                     GLDEFAVLELMDPSKDADGLHPSNLGRLVLMQPGPQPCTPRGCVELLRRYGVEINGAE
                     VVVVGRGVTVGRPIGLLLTRRTENATVTLCHTGTKDLGEHVRRADIVVAAAGVPGLIT
                     AAMVKPGAAVLDVGSTRVVGDDAKSRYVGDVDNAVREVAGWLAPMPGGVGPMTRAMLL
                     TNVVEAAESAAASRLSSSVF"
BASE COUNT          328 a          588 c          557 g          259 t
ORIGIN      
        1 cgatgagcat gaagggcctc tcgccgcgaa ccgacctggt gcgcgagtac ccacgtccgc
       61 cggtgaagcg tggctctcag cccgacgggg aagcgtcgcg gagccaaggt aatgtcacga
      121 tacgcgagcc gagtccgaaa taaacagttt cggcaattga agtcccggtt ggtgcagaag
      181 tgtgctaact gagtgttagc aatcgtaact tagggtagcc ggggatgtcg acgcgacgcg
      241 ctccctaggt gcttgtaacg agcgctagca ccagcaaacc agcgcccatg acggccatcg
      301 tcaccacgtc ggtaaaccgg gcgcgaactg ccaataggcc agctcgccgg gtcggcagca
      361 ccgaccggaa tagggcgccg gcgagcagcg atcccgcgat cagcaggttt ccccaccgaa
      421 agtggtggag cgcgatgacg accagcccgg ctccgacccc cccgatgacc aaagccagcg
      481 gtagctcgcc gaaaactccg gacttgcgca cgtgcggctc gacggcgacc gcgggatcga
      541 acgctgcaag cgcgccctcg gcttcgacaa ccaccgtcgc gccgtcagcc tcgatgacgg
      601 cctcggagtc ggtcttgacg agaacgagat ccggcgccgt ggccgcctcg gctggtgtca
      661 tgatcccgag tctaatcggc gcgacctaga agaccgaaga actcaagcgg gacgccgccg
      721 cgctctcggc cgcctcgacc acgttggtca gcagcatcgc gcgggtcatc ggacccacgc
      781 cacctggcat cggagccagc cagccggcga cctcgcgcac cgcgttgtcg acgtcgccga
      841 cgtaacggct ctttgcatcg tcgccgacaa cccgggtcga cccgacgtcg agaaccgcag
      901 cgcccggctt gaccatcgcg gcggtgatga gaccaggaac accagccgcg gcgaccacaa
      961 tgtcggcgcg ccgcacgtgc tcgcccaggt ccttcgtgcc tgtgtggcag agcgtcacgg
     1021 tcgcattctc ggtgcgacga gtcagcagca gaccgatcgg acggccgacc gtgacaccgc
     1081 gaccgacgac gacaacctcg gcgccgttga tctcgacgcc ataccggcgc agcagttcga
     1141 cgcagcctcg cggcgtgcac ggctggggcc ccggctgcat cagcacaaga cggcccagat
     1201 tgctcgggtg cagcccgtcg gcgtccttgc tcggatccat caactcgaga acggcgaact
     1261 cgtcgagccc ccgcggcagc ggcagttgaa cgatgtagcc ggtgcacctg gggtcggcgt
     1321 tgagttcgcg gacgacggcc tcgacctcag cctgcgacgc cgacgcgggc aggtttcgct
     1381 cgatgctcgc gatgccaacc tcggcacaat cgcggtgctt gccggctaca tacgcgtggc
     1441 tcgccgggtc gtcgccgaca agcaccgtgc caagccccgg tgccatgccg cggtcgcgca
     1501 aggacgcgac gcggatcttc agatcggcct tgatagccgc cgctgtcgcc ttgccgtcga
     1561 gaatgattgc cgtcacagca gcaagtcttt catgaccaag acggacgccg atgtgctggc
     1621 ctcagtcaca aaagagacgc gctgcatcag tggaagaagt gccgagtccc ggtgaaatag
     1681 agcgtgatgc cggccgcctg ggcagctgcg atcacttctg cgtcgcggat cg
//
LOCUS       DASWVF010000069         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_332869, whole genome shotgun sequence.
ACCESSION   DASWVF010000069 DASWVF010000000
VERSION     DASWVF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332869"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..382
                     /locus_tag="VGD55_01205"
     CDS             80..382
                     /locus_tag="VGD55_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868989.1"
                     /translation="MATRRTSTPAAQKAEAVAGDTVDFDFEGVSYTIEKDISLDVLDA
                     LSTINAAGDKKEQDYRTAMQCLLGLEQYASWRAKHSRLSELMALWQVAVNAVNAGK"
     gene            343..768
                     /locus_tag="VGD55_01210"
     CDS             343..768
                     /locus_tag="VGD55_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868990.1"
                     /translation="MAGGRQCGERGKIVGLVAVLDQYADAVEVDFLAHGVDLLDLWRG
                     KLSVRRAWALIEHLGPGSALFRAVDPDAAAWSLTDHLLAEILHSSNVAIWQRSGDEKA
                     PRPPRIVRPGVPDEVAASAARREARMRGFIERQRLAGVT"
     gene            768..938
                     /locus_tag="VGD55_01215"
     CDS             768..938
                     /locus_tag="VGD55_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868991.1"
                     /translation="MTPEMAKAVFSTMQELPPQEGFAWYQALSGPDQRDFLQYIVPEI
                     LERAEAQMRAEN"
     gene            938..>1518
                     /locus_tag="VGD55_01220"
     CDS             938..>1518
                     /locus_tag="VGD55_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868992.1"
                     /translation="MSDPISAGSVSVQIVPSAEGFKEKVDALTRGESVNIKLNVDTGD
                     AEAKLAAVDAETKAVGDSAGRSAVGIRAFSGGISAIGVGVAGLLPALPVLTAGVAGFG
                     GIFAAAGASAVSFGAVFETALKQATTANTELAAAQKAVNDATTSASRNKALEEQAALV
                     AQLGPNTIQLGTAIKGIDAEWTGFVNGFVPQVTS"
BASE COUNT          303 a          479 c          495 g          241 t
ORIGIN      
        1 ctcgatggcc acccgatgac gacgccacgg ccagtgctta cacaaaagcc aacacccgca
       61 acgaaaaagg agcccacaca tggccacacg ccgcacgagc accccagcag cacagaaagc
      121 cgaagcggtc gcgggagaca ccgtcgattt cgacttcgaa ggtgtgagct acacgatcga
      181 gaaggatatt tccctcgacg tgctcgacgc gctgtcgacg atcaacgcag ccggcgacaa
      241 gaaggagcag gactatcgaa cggcgatgca gtgcctgctc ggcctggagc agtacgcgag
      301 ttggcgggcg aagcattcgc gcctcagcga gttgatggca ctgtggcagg tggccgtcaa
      361 tgcggtgaac gcgggaaaat agtcggcctc gtcgccgtcc tcgatcagta cgcggacgct
      421 gtcgaggtcg atttcctcgc gcacggtgtc gacctgctcg atctgtggcg cgggaagcta
      481 tcggtgcgcc gcgcctgggc gctcatcgag catctcggcc cgggttcggc gctgttccgg
      541 gcggtcgacc ccgatgccgc ggcgtggtcg ttgaccgacc atctgcttgc ggagatcttg
      601 cactcgtcga acgtggcgat ctggcagcgc agcggcgacg agaaagcccc acgaccgccg
      661 cgcatcgtgc gaccaggtgt accggacgag gtcgccgcgt ccgctgcgcg tcgtgaggcg
      721 cgaatgcgcg gattcatcga gcggcaacga ctggcgggag tgacctgatg acgcctgaaa
      781 tggcgaaggc cgttttctcg accatgcagg aactcccgcc gcaggaaggt tttgcttggt
      841 accaggcact ttcgggtccc gatcagcgtg atttccttca atacatcgtt ccggagatac
      901 tcgagcgtgc cgaggcgcaa atgcgagctg agaactgatg agcgacccaa tttccgcggg
      961 cagcgtgtca gtacagatcg tcccgagcgc cgaaggcttc aaggagaaag tcgacgcgct
     1021 cacacgcggc gaatcggtca acatcaagct gaacgtcgac accggtgacg ccgaagcgaa
     1081 gctcgccgcc gtcgacgccg agacgaaggc cgtcggtgac agcgcgggcc gctcagcggt
     1141 aggtatacgt gcattcagcg gcggcatttc agcgatcggc gtaggtgtcg ccggtctgtt
     1201 gccggcgctc cccgtgctca ctgccggcgt cgcagggttc ggcggcatct tcgccgcggc
     1261 tggcgcgtca gcagtgtcat tcggggctgt attcgagacc gcgctgaagc aagcgacgac
     1321 cgccaacacc gaactcgccg ccgcgcagaa ggccgtcaac gacgcgacga catctgcgag
     1381 caggaacaaa gccctcgagg agcaagcggc tctcgtcgcg cagctcggcc cgaacacgat
     1441 ccaactcggc acggccatca aaggcatcga cgccgagtgg accggattcg tcaacggctt
     1501 cgtgccgcag gtcacgtc
//
LOCUS       DASWVF010000070         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_307387, whole genome shotgun sequence.
ACCESSION   DASWVF010000070 DASWVF010000000
VERSION     DASWVF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307387"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(553..1935)
                     /locus_tag="VGD55_01225"
     CDS             complement(553..1935)
                     /locus_tag="VGD55_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0868993.1"
                     /translation="MTEEFATTNYDADVSPALTAFMRDGWAPDPAPATEPAEVAPYAA
                     KRRGALSAAFPGQVIVVAAGSPSVRNNDVTYPFRASSDFVWLTGASMADAMLVMTPDA
                     EEPSSHSSTLFVRAAASRHESAQFYRDRNYGELWTGRRPGIAETQSTLGILTRPLAEL
                     DSVLKALAGAPVADKALVATALSDLRLVKDDWEIAQLSDAVAASVLGFEDVVRELDAA
                     RSTSERWIEGTFGRRARVEGNDVGYGSIAAAGDHACVLHWTSNDGPVRDGDLLLLDAG
                     VENAALYTADVTRTIPINGRFSEPQRRVYQLVYEAQSAAMEAVRPGAAFREYFRAAQQ
                     VLADGLVEWGLLPASAGDLDGPDGDLHRRYTLHGTGHMLGLDVHDCAQARSENYHEGV
                     LQPGYVLTVEPGLYFQSDDLTVPEELRGIGVRIEDDVVVTHDGCRNLTDALPRDPDAV
                     EAWMSQVLAR"
     gene            complement(2106..2330)
                     /locus_tag="VGD55_01230"
     CDS             complement(2106..2330)
                     /locus_tag="VGD55_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868994.1"
                     /translation="MTPTLLMIISANTHSAAHVLASLPKPKAKVTVPVIVILAVLVFV
                     GITRKLLKLAVIAGIICVIFLIYQSGAFSK"
BASE COUNT          459 a          786 c          820 g          440 t
ORIGIN      
        1 gacgcccacc acaaactggc gatgacgacg actacgaaca gcgcaaggcg tccgcgtcgc
       61 gcgattcttg gagccgatgg cagctgggga gtgagtcgga tcggcttagg cattgccggt
      121 tggatctgtg cgctgctcgc gggtgcggat acgacgcaag ttggggtgtc ccaatgctgc
      181 gcacgagcat gcagatcgag ccgcacggga ggtagcgaag cgagactgat cgcaacaggc
      241 cgcaccgagc gcgggtgcga cgcagtgccg ttattgcgca ccggtcgtga gtgacccggt
      301 ctcacggcgt gctgcgacga gaccgcacgg tgcgacgtaa caacccgtcg atgtgttggc
      361 cgtcgtgcac ccatcttgtg ctgcgacatt gtccccaccc cagtcgcgac cgccaagctc
      421 gtactcgcgg tagcgatgcg attttaactg cgatccacga agccaaaggg gtgaattcct
      481 agattgcaca agggtttcta cctacccacg tacaaagcgg gacattgtcg ctacgccaaa
      541 cgggtgaccg cactaacgcg cgagcacctg agacatccag gcctcgacgg catcgggatc
      601 gcgtggcaag gcgtcggtga gattgcggca gccatcgtgg gtgacaacga cgtcgtcttc
      661 aatgcggacg ccgatgccgc gcagttcttc tggcacggtc agatcatccg actggaagta
      721 cagccccggt tcgacggtca ggacgtagcc gggttgcagc acgccctcgt ggtagttctc
      781 gctacgcgcc tgcgcacagt cgtgcacatc aaggccgagc atgtgccctg tgccgtgcag
      841 cgtgtagcga cggtgcaaat cgccgtccgg gccgtcaaga tctcccgcac tcgccgggag
      901 aagtccccac tcgaccagcc catccgcgag cacttgctgt gctgcccgga agtactcacg
      961 aaaggcagcg cctggtctta ccgcttccat ggcggcactt tgagcttcgt acacgagctg
     1021 atacacgcgg cgttgaggtt cgctgaagcg gccgttgatc gggatcgttc gcgtgacgtc
     1081 agcggtgtac agggccgcat tctcgacgcc ggcgtcgagc agcagaaggt cgccgtcgcg
     1141 caccggtccg tcgttgctcg tccaatgcag cacgcacgcg tgatcgccag cagcggcgat
     1201 gctgccgtag ccgacatcgt tgccctcgac gcgcgcgcga cgtccgaagg tgccttcaat
     1261 ccagcgctcg ctcgtggacc gcgctgcatc gagttcgcgc acgacgtctt cgaacccgag
     1321 cacgctcgca gcgaccgcat cggatagctg cgcgatctcc caatcgtcct tcactaggcg
     1381 caggtcggaa agtgcggtag cgacgagcgc cttgtcggca acgggtgcac ctgccaacgc
     1441 cttcaacacg ctgtcaagct cagccaacgg acgagtgagt atgcccaggg tcgattgtgt
     1501 ttcggcaatg cctggacgcc ggcctgtcca tagctcgcca tagtttcgat cgcggtagaa
     1561 ctgcgcgctc tcgtgccgcg atgcggcagc gcgcacgaag agggtcgagc tgtgactgct
     1621 gggttcctcg gcatcgggcg tcatgacgag catagcatcg gccatgctcg cgcccgtgag
     1681 ccacacgaaa tcgctgctcg cccggaaggg gtaggtcacg tcgttgttgc gaacgctcgg
     1741 cgagccggcc gcgacgacga tcacctgccc ggggaaagct gccgacagcg caccgcgacg
     1801 cttggcggcg tagggcgcaa cctcggccgg ttcggttgcg ggtgccgggt caggcgccca
     1861 gccgtcgcgc atgaaggcgg tgagcgcggg ggacacgtcg gcgtcgtagt tcgtcgttgc
     1921 gaattcttcg gtcatggggc catggtagga ggacgccggg gccccgcgag cgtgactcgg
     1981 atacagcgcg tgcaattact accccgtgag gacagcaatc gcacgcgcta gccgccgccg
     2041 ctcagccgca acgtccgtca gccctttcga gtctgctgct gtcgaactcc cgccgcaaac
     2101 cggcattact tgctgaacgc gcccgattgg tagatcagaa agatgacgca gatgatcccg
     2161 gcgatgaccg ccagcttgag gagcttgcgg gtgatcccga cgaagaccag aaccgcgagg
     2221 atcacgatga ccggcaccgt caccttggcc ttcggcttgg gtagcgacgc gagcacgtgc
     2281 gcggccgaat gagtgttcgc cgagatgatc atgagcaggg tgggggtcat ggtcggatca
     2341 tctcagccca gcgcggcttg gacgagttct gtcagcacac ctgacaggtc tttcgggtgc
     2401 acgaatgcga tcgaggcacc gagcgagccg tgtcgaggtt gctcgtcgag aatccgaacg
     2461 ccctgtgcgg caagcgtttc catcgcgagc cggacgtcgt cgacc
//
LOCUS       DASWVF010000071         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_307445, whole genome shotgun sequence.
ACCESSION   DASWVF010000071 DASWVF010000000
VERSION     DASWVF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307445"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGD55_01235"
     CDS             <1..426
                     /locus_tag="VGD55_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY0868995.1"
                     /translation="MRAIAYARATRPSVLEAVTVNVDTEETEALEAEWERRGIPVPLK
                     VLDSPYREVTRTVIEYVRSIRRSSPRDVVTVFIPEYVVGHWWEHLLHNQSALRLKGRL
                     LFSPGVMVVSVPWQLQSSIGIADSLDSLDASGDDLPTSA"
     gene            423..1631
                     /locus_tag="VGD55_01240"
     CDS             423..1631
                     /locus_tag="VGD55_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY0868996.1"
                     /translation="MNPAGALVGTLIELDVGAPAHGGSCVARHDGQVMFVRHALPGER
                     VVARVTEQSKHFGRADAVEVLAASEHRVVPPCPAARPGGCGGCDWQHATAAFQREIKA
                     TVVMEQLLRLAGIDREVVVEALPGDGFGWRTRVRFAIDASGRAGFRRHRSHQVEVVGG
                     CPIAHPLVEAADVEGKRWDAISEVEVAVSVASGKRQVRSAAGRDGAMRVREGGPLEEA
                     AAGRTWRVSPGGFWQVHPAAADVLTEAVMTGLLPRPGERALDLYAGVGLFAGSLADAV
                     GVTGNVIAVESSSVAAMDASRNLADLPQAQLVRADVEKALGRPEQFGHVDLIVLDPPR
                     SGAGARVVAGVAGLHPRAAAYVACDPAALARDLATFAEHGYQLTHLRGFDMFPQTHHV
                     ECVAILEATA"
     gene            1628..2065
                     /locus_tag="VGD55_01245"
     CDS             1628..2065
                     /locus_tag="VGD55_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868997.1"
                     /translation="MKATLILCDAAQVDPAGKVHILGAGWTVVSVAPGAPVLAHAIVA
                     IVHVPWHQTGEPHRLRLSLEDADGRAVMIGPAPDQLAPLVHEQLIEVRRPPEAPEGIT
                     LDVPVVVSVGPGMPLTDGRYSWRLEIDGASDEDWLASFHVRIG"
     gene            complement(2111..2503)
                     /locus_tag="VGD55_01250"
     CDS             complement(2111..2503)
                     /locus_tag="VGD55_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0868998.1"
                     /translation="MQMRLELIPLPVSDVDRAIAFFADKLGFKKDFDVRPSEGVRVVQ
                     FTPEGSGCSVGIGTGLDVYAGEPGSIRGLHLVVEDITEARAEFVSKGVEVSEIHDFGG
                     GVRGANFSDPDGNTFELQEMSWRKGETY"
BASE COUNT          410 a          885 c          918 g          463 t
ORIGIN      
        1 atgcgtgcca tcgcgtacgc gcgcgccacg cgaccctcgg tgctcgaggc tgtcaccgtc
       61 aacgtcgaca ccgaggagac cgaagcactc gaggcagagt gggaacggcg agggattccg
      121 gtgccgctga aggtgctcga ctcgccctac cgcgaagtga cgcgcacagt catcgagtac
      181 gtgcgttcga tccggcgtag cagcccgcgc gacgtcgtca ctgtgttcat ccccgaatac
      241 gtcgtcggcc attggtggga acacctgttg cacaaccaga gcgcgctgcg attgaagggt
      301 cgattgctgt tctcgcccgg cgtcatggtc gtgagcgtgc cgtggcaact gcagtcgtcg
      361 atcggcatcg ccgacagcct cgacagcctc gacgcgtcgg gtgacgacct gccgacgagc
      421 gcgtgaaccc cgccggggcg ctcgtcggca cgctgatcga gctcgatgtg ggcgctcctg
      481 cgcacggcgg atcctgcgtg gcacggcacg acggccaggt gatgttcgtg cggcacgccc
      541 tgccgggaga acgcgtcgta gcgcgcgtca ccgagcagtc gaagcacttc ggtcgcgccg
      601 acgctgtcga ggtattggca gcgtccgagc accgcgtcgt cccgccgtgt cctgctgctc
      661 ggcctggagg ttgtggcggc tgcgattggc aacacgcgac tgccgcgttc cagcgcgaga
      721 tcaaggcgac cgtggtcatg gagcaactgc tgcggctggc cgggatcgat cgcgaggttg
      781 tcgtcgaggc actgcctggc gacgggttcg gctggcgcac ccgcgtgcgg ttcgcgatcg
      841 atgcgtcagg gcgtgcaggg tttcggcggc atcgctcgca ccaggtcgag gtcgtcggcg
      901 gctgcccgat tgcgcatccg ctggtcgagg cggctgacgt cgagggcaag cgatgggacg
      961 ccatcagcga ggtcgaagtc gccgtgtccg tcgcgtcggg caagcggcag gtgcgcagcg
     1021 cggccggtcg tgatggcgcg atgcgggtac gcgagggcgg cccgctcgag gaagccgcgg
     1081 ccgggcgcac gtggcgggtc agccccggcg ggttctggca ggtgcacccg gctgcggccg
     1141 acgtcctgac ggaagcggtc atgacagggc tgctccccag gccgggtgag cgggcgctcg
     1201 acctctacgc cggagtgggc ttgttcgcgg gctccctggc tgatgcagtc ggagtcaccg
     1261 gcaacgtcat cgcggtcgag tcgtcgtcgg tcgccgcgat ggatgcgtca agaaatctcg
     1321 ccgacctgcc gcaggcgcaa ctcgtgcgcg ccgacgtcga gaaagcgctc ggccggcccg
     1381 agcaattcgg ccacgtcgac ctgatcgtgc tcgacccccc gaggtcgggg gcgggtgcgc
     1441 gcgtcgtcgc gggtgtcgcc ggcctgcacc cccgcgcggc cgcctacgta gcgtgtgatc
     1501 cagcggcatt ggcgcgcgat ctcgccacgt tcgcggaaca cggatatcag ctgacacacc
     1561 tgcgcgggtt cgacatgttc ccgcagaccc atcacgtcga atgtgtggcg atcctggagg
     1621 caaccgcgtg aaagccaccc tcatcttgtg cgatgcggcg caggtcgatc cggccggcaa
     1681 ggtgcacatc ctcggggcgg gctggaccgt cgtctcggtc gcgccgggcg cacccgtgct
     1741 cgcgcacgcg atcgtcgcga tcgtgcacgt cccgtggcat cagaccggcg aaccgcatcg
     1801 cctgcggctt tctctcgagg acgccgatgg gcgggccgtg atgatcgggc ctgcgcccga
     1861 ccaactggca cccctcgtgc acgagcagct gatcgaggtt cgccgaccac ccgaggcgcc
     1921 agaggggatc acgctcgacg tgcctgttgt cgtctcggtg ggtcccggca tgccgttgac
     1981 ggatggtcgc tatagctggc gtcttgagat cgacggagca agcgacgaag actggctggc
     2041 cagcttccac gtcaggatcg gctgagcacg gcccctcagg ctccgacgct cacggcgctc
     2101 agtgagcgcg ctagtacgtc tcgcccttgc gccacgacat ctcctgcaac tcgaatgtgt
     2161 tgccgtcggg atcggagaag ttcgctcctc tcacgccgcc cccgaagtcg tggatttcgc
     2221 tgacctccac acctttgctg acgaactcag cacgcgcctc ggtgatgtcc tcgacaacca
     2281 ggtgcaggcc tctgatgctg ccgggttctc ccgcgtacac atccagccct gtccctattc
     2341 caaccgaaca accggaaccc tccggcgtga actgcacgac ccggacccct tcgctgggtc
     2401 gtacgtcaaa gtccttcttg aacccgagct tgtcagcaaa gaaagcgatg gctcgatcga
     2461 cgtccgaaac cgggagcggg atcagttcca gcctcatctg catcgcggtt gcaccccctc
     2521 gggtttgttc tcgtccgcgc gatgattcca caccgccgcc gaccagtacg gtggtcagta
     2581 gtcacggtcg ggatcgggcg gaggagttcg gtggatagct tcggtagtcg cgcgacattg
     2641 caggtcggct cgagctcgta tgagatctac cggctt
//
LOCUS       DASWVF010000072         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_307476, whole genome shotgun sequence.
ACCESSION   DASWVF010000072 DASWVF010000000
VERSION     DASWVF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307476"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGD55_01255"
     CDS             <1..322
                     /locus_tag="VGD55_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0868999.1"
                     /translation="AQLGNPVRADTTGGILNQVTLPNAAALAAMTNGGDGATATFTNA
                     TNQWATNPLTVNGNGIPVRNPATVNSTGQFGTNTGTPDIVVLDTGASVTFIYDKTNVC
                     WQVG"
     gene            327..>1874
                     /locus_tag="VGD55_01260"
     CDS             327..>1874
                     /locus_tag="VGD55_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0869000.1"
                     /translation="MIDPRKYARDLALFAVAAVAILLSTLALLRSPPAPVLVLDEAGA
                     AHRILLGSYPVGGQTTVPLANGLNSNIATQGLSSIRIVGPTAPFTLGGFTPGQAGNEL
                     LVTNTTAQPMTVLPGDVSTTAKGQIDTQSGDGGLTLQPKPGALRFIYDGQEGKWVLSS
                     TGPQRALVCNVLDYGADPGDTVDSTSAFTKALAACSAGGETRVPGGTYKLSGQLAVPV
                     GETMRGDGMNLTTLKWTSATNGLALASGSVVLGGQTISDMALNGSGAGLVGLYVDNRY
                     QVRIDRLQITGWTQQGEYWLNSYTSESVGCMLSNNGGRPAAVPITSVVPGNPTQLGVA
                     IDAGFPTGNVVVLGVGDGGVAGGYDTGPNGNWAVTQVDAGFWSIPFASDGGTNLTLNT
                     ATAQDLGWAQVELDGCTTWRWEHDYISGGNATTPAGFRTDRSSVINLDEGAIESTGVN
                     VQLGAKGENTNGASLINMRGVDMEGIPTTQAIEAGYGWHGATQGGSIFGLNVQGCSFA
                     NGVQAYDL"
BASE COUNT          334 a          644 c          649 g          247 t
ORIGIN      
        1 cgcgcagctc ggcaacccgg tgcgggccga cacgacgggc ggcatcctca atcaggtcac
       61 gctgccgaac gccgcggcgc tggccgcgat gaccaatggt ggcgacgggg cgaccgccac
      121 gttcacgaat gcgaccaatc agtgggcgac gaacccgctc accgtgaacg gcaacggcat
      181 accggtgcgc aatccggcga ccgtgaactc cacggggcag ttcggcacga acaccgggac
      241 gccggacatc gtggtgctcg acaccggcgc gagcgtgacc ttcatctacg acaaaacgaa
      301 cgtctgctgg caggtgggct gacccgatga tcgatcctcg caagtacgcg cgcgacctgg
      361 cgctgttcgc ggtcgctgca gtggccatcc tgctttcgac gctggccctc ctgcggtcgc
      421 cgccggcccc ggtcctcgtt ctcgacgaag caggagcggc gcaccggatc ctgctcggca
      481 gctacccggt cggcggtcag acgaccgtcc cgctcgcgaa tgggctcaac agcaacatcg
      541 cgacgcaggg cctgagctcg atccggatcg tcgggccgac cgcacccttc acgctgggag
      601 gcttcacgcc ggggcaggcc ggtaacgagc tcttggtcac gaacacgacc gcgcagccca
      661 tgacggtgct ccctggcgac gtgagcacca cggctaaggg gcagatcgat acgcagagcg
      721 gcgacggtgg tctcacgctg cagccaaagc cgggcgccct gcgcttcatc tacgacgggc
      781 aggagggcaa gtgggtgcta tcgagcaccg ggccgcagag ggcgctcgtt tgcaacgtcc
      841 tcgactacgg cgcggacccg ggcgacacgg tcgactcgac gtccgctttc acgaaggcac
      901 tcgcggcatg cagcgccggc ggcgagacgc gcgtccctgg cggcacgtac aagctctcgg
      961 gtcagctcgc cgtccccgtg ggcgagacga tgcgcggcga cggcatgaac ctcaccacgc
     1021 tcaagtggac gagcgcgacg aacgggctcg cgctcgcgtc ggggagcgtc gtgctcggcg
     1081 gacagacgat ttcggacatg gccctcaacg gcagcggcgc cgggctggtc ggcctctatg
     1141 tcgacaaccg gtaccaggtc cggatcgatc gtctgcagat caccggctgg acgcagcagg
     1201 gggaatactg gctcaactcc tacacgagcg agtcggtcgg ctgcatgctg agcaacaacg
     1261 gcggccgccc ggcggcggtc cccatcacca gcgtcgtacc aggcaacccc acgcagctcg
     1321 gggtcgcgat cgacgcgggc ttccccaccg gcaacgtcgt cgtgctcggc gtgggcgacg
     1381 gcggcgtggc gggcgggtac gacactggac ccaacggcaa ctgggccgtc acccaggtcg
     1441 atgcgggctt ctggagcatc ccgttcgcaa gcgacggcgg gacgaacctc acgctcaaca
     1501 ccgccacggc gcaggatctc ggctgggcgc aggtcgagct cgacgggtgc acgacgtggc
     1561 ggtgggagca cgactacatc agcggcggca acgccacgac cccagcgggc tttcgcaccg
     1621 accgcagcag cgtcatcaac ctcgacgagg gggccatcga gagcaccggc gtgaacgtgc
     1681 agctcggggc gaagggcgag aacacgaacg gcgcctccct catcaacatg cgcggcgtcg
     1741 acatggaggg gatcccgacg acgcaggcca tcgaggcggg gtacgggtgg cacggcgcca
     1801 ctcagggcgg cagcatcttc gggctgaacg tccagggctg cagcttcgcc aacggcgtcc
     1861 aggcgtacga cctg
//
LOCUS       DASWVF010000073         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_434993, whole genome shotgun sequence.
ACCESSION   DASWVF010000073 DASWVF010000000
VERSION     DASWVF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434993"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VGD55_01265"
     CDS             <1..223
                     /locus_tag="VGD55_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0869001.1"
                     /translation="DLLLAEAGFAEGMPPAMHLRGIEGGQLAADADVGQLVVTHVAPW
                     DDSAAALAAAREMFVGSTTLARPGAVYEL"
     gene            256..987
                     /gene="rph"
                     /locus_tag="VGD55_01270"
     CDS             256..987
                     /gene="rph"
                     /locus_tag="VGD55_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0869002.1"
                     /translation="MPTTSRLDGRANDELRSVTITRHWLDHAEGSVLVEFGRTRVLCA
                     ASAAEGVPRWRKGSGLGWVTGEYAMLPRATTTRNERESVKGRLGGRTQEISRLVGRAL
                     RACIDYKALGEATIQLDCDVLQADGGTRTAAITGAYVAMHDAIDWLRKNGKMPGEPLI
                     RSVSAVSVGVIDGKPRLDLNYEEDVRAETDMNMVVTGDGEFIEVQGTAERNPLRRAEI
                     DALLDLAVGGCASLDALQRAALAAL"
     gene            984..1598
                     /gene="rdgB"
                     /locus_tag="VGD55_01275"
     CDS             984..1598
                     /gene="rdgB"
                     /locus_tag="VGD55_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0869003.1"
                     /translation="MTEGRVVLATRNAHKVVELRRILSAAGLESVELIGLDAYPDLPE
                     VPETGATFEANALLKAHAVASATGLTAIADDSGLAVDALNGMPGVLSARWSGGLGDQA
                     NLQLVLAQIGDVPDDRLGASFECAAAAVFPDGAEVVVRGQLTGSIIRTPRGANRFGYD
                     PIFVPNGDSRTTAELDPDEKDAISHRGVAFRALATRLHEIWSAD"
     gene            complement(1926..2597)
                     /locus_tag="VGD55_01280"
     CDS             complement(1926..2597)
                     /locus_tag="VGD55_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEY0869004.1"
                     /translation="MTAPQSVGEVRELVANLHGELLRWGLVVWTAGNVSARVPGEDLF
                     VIKPSGVSYDELTAAKMITCDLDGHVVDGNHSPSSDTAAHAYVYRAMPDVGGIVHTHS
                     TYATAWAARGEAIPCVLTGMADEFGGEIPIGPFALIGNDDIGRGIVETLSGHRSSAVL
                     MRSHGVFTIGVDATAAVKSAVMCEDVARTIHIARTLGEPLKIEPADIDRLHDRYHTVY
                     GQRGV"
     gene            complement(2594..>3342)
                     /locus_tag="VGD55_01285"
     CDS             complement(2594..>3342)
                     /locus_tag="VGD55_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY0869005.1"
                     /translation="LAEVPGMCGVVENGIVQGLWGYEAGQSGVGDIFAWFVENVLPAD
                     YVARATELGMDAHEYLSSICSRQRVGAHGLVALDWESGNRSVLVDHSLSGVILGLTLS
                     TTPEDIYRALVESTAFGTRKIIETFEEAGVPCEEFVVAGGLLKNAFVMQVYADVTGKA
                     MSLLDSENGPALGSAIHAAVAAGAYPDVTTASAAMGRVLPNAYLPNAANHEIYNRLYA
                     EYSALHDYFGRGVNDVLHRLHDLRIEASAP"
BASE COUNT          571 a         1076 c         1129 g          566 t
ORIGIN      
        1 cgatctgctg ttggcggagg caggcttcgc cgaaggtatg ccgcccgcga tgcacctgcg
       61 cggcattgaa ggcggccagt tggcagccga cgcggatgtc ggtcagctcg tcgtgacgca
      121 tgtcgcaccg tgggacgact cggccgccgc acttgccgcc gcccgcgaga tgttcgtcgg
      181 gtcgacgacg cttgctcgcc cgggagctgt gtacgagctg taggcgctca gcgcgcgacg
      241 gatagggtcg atcgcatgcc tacgactagc cgcctcgacg gccgcgcgaa cgacgaactc
      301 cgctcggtga ccatcacccg ccattggctc gaccatgccg aaggttcggt gctggtcgag
      361 ttcggccgta cccgggtgct gtgtgcagcg agtgccgccg aaggtgtgcc gcgttggcgc
      421 aagggcagcg gccttggctg ggtgacgggc gaatacgcga tgctgccgcg cgcaacgacc
      481 actcgcaacg aacgtgaatc ggtaaagggc cggctgggcg ggcgcactca ggagatctca
      541 cgactggtcg ggcgcgcact gcgcgcgtgc attgactaca aggcactcgg cgaggcgacg
      601 atccaactcg actgcgatgt cttgcaggcg gacggcggca ccagaaccgc cgcgatcact
      661 ggcgcctacg tagcgatgca cgacgcaatc gattggctgc gcaagaacgg caagatgccg
      721 ggtgagccgc tgatccgatc ggtctcggcc gtcagtgtcg gcgtcatcga cggcaagccg
      781 cggctcgatc tgaactatga ggaagatgtg cgcgccgaga ccgacatgaa catggtggtc
      841 accggcgatg gcgagttcat cgaggtgcag ggcacggccg agcgcaaccc gttgcgcagg
      901 gcggagattg acgcgctgct cgaccttgcg gtcggcggtt gtgcgagcct cgacgctttg
      961 cagcgagcag cactggccgc gctgtgaccg aaggccgcgt ggtgctcgcg acccgcaatg
     1021 cgcacaaggt cgtcgagctg cgtcgcatct tgtcggcagc cgggctcgag tcggttgagc
     1081 tgatcgggct cgacgcatat cccgacctgc ccgaggtgcc ggaaaccggc gcgacgttcg
     1141 aggccaacgc actgctgaag gcgcatgcgg ttgctagcgc taccggtctt accgcaatag
     1201 ctgatgactc gggtctcgcc gtcgatgcgc tcaacggcat gccgggtgtg ctctccgcca
     1261 ggtggtcggg aggtctcggc gaccaggcca acctgcagct ggtgctcgcc cagatcggcg
     1321 acgtgcctga cgaccggctc ggtgcgtcat tcgagtgcgc cgccgcagca gtttttccgg
     1381 acggagcaga agtcgtggtc cgcggccagt tgaccggcag catcatccga acgccgcggg
     1441 gtgccaacag gttcggctac gacccgatct tcgttccgaa cggcgactcg cgtacgacgg
     1501 ccgaactcga cccggacgag aaggacgcga tcagccatcg cggcgtcgcc ttccgggcgc
     1561 tcgccacgcg actccatgag atctggtccg cagactgacc tccctgccgg cggcctgccg
     1621 ctgctcgttg gcgggtgaga aaaatcccgc gggagacgta tcagtgagcc aggtcgaggc
     1681 tctttcttcg tgacggccga cctggggggc gaccgccact cggtgtgaac cggagcatgg
     1741 gcgcgctcgc tacgggggat tgcaccgaga acgcgttggg gggcgccggg atcatcggtc
     1801 ccggcgccct cagaccgaga ccgttgatgc ttaacggtcg caagaacggc cgctgagcat
     1861 caacacccag cgccgacgcc gcgccccgcg cgcacccggc gtccgctctg attcagcgct
     1921 gaccgttaca ccccgcgttg tccgtagacg gtgtggtagc ggtcgtggag gcggtcgata
     1981 tcggcgggct cgatcttcag cggttcgccg agcgtgcgcg cgatgtggat cgtccgcgcg
     2041 acgtcttcac acatgaccgc cgacttcacg gccgccgtcg catcgacacc gatggtgaag
     2101 acgccgtggc tgcgcatcag aaccgcggac gaccggtgcc cgctcagagt ctcgacgatg
     2161 ccgcgcccga tgtcgtcgtt gccgatcaag gcgaagggac cgatcggtat ctcgccacca
     2221 aactcatcgg ccattccggt gagcacgcac gggatcgcct cgcctcgagc cgcccatgcg
     2281 gtggcgtatg tcgagtgcgt gtggacgatg ccgccgacat cgggcatcgc tcggtagacg
     2341 tatgcgtgag cggcggtgtc actggacggc gagtggttac cgtcgacgac gtgcccgtcg
     2401 aggtcgcagg tgatcatttt cgctgcggtg agttcgtcgt agctgacgcc gctgggtttg
     2461 atgacgaaca ggtcctcacc aggaacccgt gccgagacat tgccggcggt ccagacgacg
     2521 agtccccatc gcagcaactc cccgtgcagg ttcgcgacca actcacggac ctcgccgacc
     2581 gattgcggcg ctgtcacggt gcgctcgctt cgatgcgcag gtcgtgcaac ctgtgcagca
     2641 cgtcgttcac gccgcgaccg aagtaatcgt gcaacgccga gtactccgca tagaggcggt
     2701 tgtagatctc gtggtttgcc gcgttgggca ggtaggcgtt gggtagcacg cggcccatgg
     2761 ccgccgatgc cgtggtgacg tcggggtacg cgccggcagc gaccgcagca tgaatggcag
     2821 aaccgagcgc tggcccgttc tccgaatcga gcagcgacat cgcctttccg gtcacgtcgg
     2881 cgtacacctg catcacgaat gcattcttca gcaagccgcc ggcgacgacg aactcctcgc
     2941 agggcacgcc agcctcctcg aacgtttcga tgatcttgcg ggtgccgaat gcggtcgatt
     3001 cgacgagtgc gcggtagatg tcttcgggtg tggtcgacaa ggtgagaccg aggatgacac
     3061 cggacagcga atggtcgacg agcaccgacc gattgccgct ctcccagtcg agggcgacga
     3121 ggccgtgcgc acctacccgc tgccgcgaac agatcgaact gaggtactcg tgcgcatcca
     3181 tcccaagttc tgttgcgcgg gcgacgtaat cggcgggcag tacgttctcg acaaaccaag
     3241 cgaagatgtc accaacaccg ctctgaccag cttcgtatcc ccacaggcct tgcacgatgc
     3301 cgttttcgac gaccccgcac atgcccggta cctcggcgag cc
//
LOCUS       DASWVF010000074         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_205860, whole genome shotgun sequence.
ACCESSION   DASWVF010000074 DASWVF010000000
VERSION     DASWVF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205860"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1207)
                     /locus_tag="VGD55_01290"
     CDS             complement(146..1207)
                     /locus_tag="VGD55_01290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869006.1"
                     /translation="MTAAPLMAASTDLLAGNTGVQIVAGIERLAAHRGELEALAQRCS
                     APVTARSTWILSSAVSGPQPWAVLVRDPAGFLRAGAVLIEVDGVERGSKIVTFAGSDL
                     GHRGTILADDRLWARRLGIALSRTLPARTSGWAVELGPIDADAATLSGFTAGFPQLVK
                     MDADPIPMIRRELGLDQALDYLTPAMRRTLRKATNRLTSDGYELDVQFTRSHSEIRAL
                     MPELEESHRNRDHDRGRASDLDEQDGLRAWRSRLTDLSLDGLLEVAIARIDGALAAHV
                     VGVRDGATYRVLEGHYVTAWGRYAPGRLLEAAVVQRMLDEPDMIALDWMTSVAPESLL
                     ATNASAPMVVLRAGLGRRA"
     gene            complement(1204..>3171)
                     /locus_tag="VGD55_01295"
     CDS             complement(1204..>3171)
                     /locus_tag="VGD55_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0869007.1"
                     /translation="ALLLVVRDIADRLPLSLWLDGIVGGLAVAAIAAAAVGPVLKAAH
                     ANGDSTAAIVTTEAYPLLDVLLLLVVTALLALFHWKPPAGLWFLAGGLVLFSVADGAY
                     LFLVAHNSYQSGGPDDLVWVLATLAMGFAPGWSKRAAGASLPGWLLLGIPVGATLWAV
                     ALLVYDHASPLHPIAVALAAATIVAAIGRLIVSFREVTTLAHSHQLALTDELTGLGNR
                     RAFYEHVDACLTAEPDRNGALLLLDLDRFKEVNDSLGHHAGDDLLLQVATRLTQGLTH
                     EEGLLARLGGDEFSIFVVDVDREGAEMFAEMVRETLLAPFTIDGITVRVAASVGISLF
                     PRHGLEVSTLLRRADIAMYHAKDLRTGYRVYSESDDSLGGQDRLRTLEELRQVVHARK
                     LAMHYQPKVDSQTSRVSGVEALVRWDHPVHGLLLPDAFLPLAEDAGLMRDLTMAVLEQ
                     SLDQVVLWRAGGRMLSVAVNLSASSLVDLELPDRVHQLLVNRGLPASALELEITEDFL
                     MGDRERAREILTQLRTLGIRVAVDDFGTGYSSLAYLRELPIDELKLDRSFVQPMAEDP
                     RAAAIVRSTISLAHSLGMRLVAEGVENEITAGHLALSGCDVSQGFFFSRALPPLELER
                     WLDDRPDIVSMQTAATARRTGSNHEANGLAS"
BASE COUNT          553 a          997 c         1143 g          478 t
ORIGIN      
        1 acatggattg gcagcagtgg gtcgacggaa gtgcgacgaa tgcagatgcc gtcgcgagca
       61 gcgcgcctgc ctctagtagc accccgctgg cgagctacgg tccgacggca acgccgcctg
      121 cgaataacta gaccagcaaa accgatcagg cccgtcggcc gagtcccgcg cgtagcacga
      181 ccatgggagc gcttgcgttt gtggcaagca acgactcggg ggcgaccgag gtcatccagt
      241 cgagtgcgat catgtcgggc tcgtccagca ttcgctgcac gacggccgct tcgaggaggc
      301 ggccgggcgc gtaacgtccc cacgcggtga cgtagtggcc ttcgagaacc cggtatgtcg
      361 cgccgtccct gacacccacg acgtgcgcgg cgagcgcgcc gtcgatgcgc gcaatcgcga
      421 cctcgaggag accgtcgagc gacaggtcgg tcagtctgct gcgccaggcg cgcaggccgt
      481 cttgctcgtc gaggtcgctc gcgcgcccgc ggtcgtgatc gcgattgcgg tgcgactcct
      541 cgagttcggg catgagcgcg cggatctcgc tgtgactacg cgtgaactgg acgtcgagtt
      601 cgtagccgtc gctcgtgagc ctgttagtcg ccttgcgcaa cgtgcggcgc atcgccgggg
      661 tgaggtagtc gagcgcctgg tcgagaccga gttcgcggcg aatcatcggg atcggatcgg
      721 cgtccatctt gaccaactgt gggaagccag cggtgaagcc gctcagtgtc gctgcgtcgg
      781 cgtcgatcgg gccaagttcg accgcccacc cgctcgtgcg cgcaggcagg gtgcgtgaca
      841 aggcgatgcc gagccggcga gcccacagcc ggtcgtcggc gaggatcgtg ccgcggtgcc
      901 cgaggtcgct gccggcgaac gtgacgatct tggagccgcg ctcgacgccg tcgacttcga
      961 taagaacagc gccggcacga aggaagcccg cgggatcgcg cacgagcacg gcccatggct
     1021 gggggccact gaccgcgctc gacagaatcc aggtgctccg ggcggtgaca ggggcgctgc
     1081 atcgctgagc aagtgcttcg agctctccgc ggtgggcagc caaacgctca atgcccgcaa
     1141 cgatctggac gccggtgttg ccagcgagca gatcggtgct ggccgccatg aggggcgcag
     1201 cggtcacgag gccagcccgt tcgcctcgtg gttgctgccg gtccggcgag cagtggcagc
     1261 ggtttgcatg ctgacgatgt cgggccgatc gtcgagccag cgttcgagtt cgaggggtgg
     1321 caacgcacgg gagaagaaga agccttgcga gacgtcgcac ccggaaagcg cgagatgacc
     1381 tgcggtgatc tcgttctcga cgccctcggc gacgagtcgc atgccgaggg agtgcgcgag
     1441 gctgatcgtc gagcggacga tggccgcggc acgcgggtct tcggccatcg gctgcacgaa
     1501 ggagcggtcg agcttcagct cgtcgatcgg cagttcgcgc aaataggcca gcgatgagta
     1561 gccggtaccg aagtcgtcga cagcgacccg gatcccgagc gtgcgcaact gcgtcagaat
     1621 ctcgcgcgcc cgctcgcggt cacccatgag gaagtcttcg gtgatttcca gttctagagc
     1681 ggacgccggg aggccgcggt tcaccagcaa ctgatgcacc cggtcaggta gttcaaggtc
     1741 gaccagcgag gacgccgaca ggttgaccgc gacactgagc atgcggccac cggcgcgcca
     1801 caagaccacc tggtcgagtg attgctcgag caccgccatc gtgaggtcgc gcatcagacc
     1861 ggcgtcctcg gccaacggca agaaggcatc gggcagcagc aagccgtgca ccgggtgatc
     1921 ccagcgaacc agggcttcga cgccggagac tcgggacgtc tgcgagtcga ccttcggctg
     1981 gtagtgcatg gcgagcttgc gtgcatgcac gacctgacgc agttcttcca gtgtccgcag
     2041 tcggtcctgg ccgccgagtg agtcgtcgga ttcgctgtag acgcggtagc cggtgcgcaa
     2101 atccttggcg tggtacatcg cgatgtcggc gcgacgcagc agcgtcgaaa cctcgaggcc
     2161 gtgccgcggg aagagcgaga tgccgacgct cgccgcgacg cgcacggtga tgccgtcgat
     2221 cgtgaacggt gcgagcagcg tctcccgcac catctcggcg aacatctcgg cgccttcgcg
     2281 atcgacgtcg acgacgaaga tcgagaactc gtcaccacct aggcgggcga gcaggccctc
     2341 ctcgtgcgtg aggccttgcg taaggcgcgt ggcgacctgg agcaggaggt cgtcgccggc
     2401 gtggtggccg agtgagtcgt tgacttcctt gaaccggtct aggtcgagaa gtagcagcgc
     2461 gccgttccgg tcgggttcgg ccgtcaggca cgcgtcgacg tgctcgtaga aggcacgccg
     2521 gttgccgagg cccgtgagct cgtcggtgag cgcaagttgg tgactgtgcg ccagcgttgt
     2581 tacctcgcgg aagctgacga tgaggcggcc gatcgcggcg acgatggtgg cagcggcgag
     2641 cgcgacggcg atggggtgca ggggcgaagc gtggtcgtaa acgagcagcg cgaccgccca
     2701 caacgtcgcg ccgaccggga tgccgagcag cagccagccg ggcaggcttg cgccagcggc
     2761 tcgcttcgac caccccgggg cgaagcccat ggccagtgtc gcgaggaccc agacgaggtc
     2821 gtcaggtccg cctgactggt agctgttgtg cgcgaccagg aacaggtacg cgccgtcggc
     2881 gaccgagaac agcacgagac cgccagcgag gaaccacaaa cccgccggtg gcttccagtg
     2941 gaacagcgcg agcagcgcag tgacgacaag cagcagcagg acgtcgagca gcgggtaggc
     3001 ctcagtggtg acgatggctg cggtgctgtc gccgttggcg tgcgctgcct tcaggaccgg
     3061 accgactgcc gctgccgcga tcgccgcgac cgcgaggccg ccgacgatgc cgtcgagcca
     3121 caggctcagc gggagtcgat cagcgatgtc gcgcacgacc aggaggagtg c
//
LOCUS       DASWVF010000075         5374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_409670, whole genome shotgun sequence.
ACCESSION   DASWVF010000075 DASWVF010000000
VERSION     DASWVF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409670"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGD55_01300"
     CDS             complement(<1..263)
                     /locus_tag="VGD55_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869008.1"
                     /translation="MPGSASVRAVLADVLVAGERGAPAADRLCIACLPWLGVDAAAVS
                     LIDEGESRGTFGASDPAARLLDGLQFTAGEGPCLEAAQTRNPVL"
     gene            complement(777..2093)
                     /locus_tag="VGD55_01305"
     CDS             complement(777..2093)
                     /locus_tag="VGD55_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0869009.1"
                     /translation="MSGGRRQPPTAPALPATDRGRWLWLAPPPVARDLAGWALVPLRA
                     FLGFTFCFAGLQKLANPAFFKASNPASIQAQMAGAARRSPIHALITPLSHHAALLGAV
                     IALGEIAVGVGALLGLLTRIAAIGGLLLSLNLFLAVSFHTNPYYTGSDIVFVFAWMPL
                     IIAGGGQLSLDALTSNLIAARHLRPAQLPVQLPFATVQSICGHYHGGRCEAQHNAACQ
                     PAGCPYLARELSKPARGAAAAKLDVDRRRLVGALTLGAGAVAVLGTAAIAGVARLVHG
                     SSSASAAVPSLAGSTPATAAPSEAATSAPTDTGTPSSSGTPTPTSKATATTGTKSAGT
                     KPTGTKIGAASAVPVKGSATFQDPATGDPAIVVQPRAGTFLGFDAVCPHAGCTVAYDE
                     GSLTFMCPCHGSQFNGSTGAVEVGPASRGLGRITVAEGPDGQLYAR"
     gene            complement(2184..3440)
                     /locus_tag="VGD55_01310"
     CDS             complement(2184..3440)
                     /locus_tag="VGD55_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0869010.1"
                     /translation="MKTTVPLRPALRVAAAATLAVALCYIVIVAGILALVSNRLLHET
                     DNRLTELLRQTRNVSVAEFLAKASIHDADDPPAYVWEVRGGAVVATSAGAPALPSTLV
                     NGSQARYRSVSIAGHAFRFRHARQPDGSTLYAAESLVQQHHVRSVLLMAAFAVSPLLL
                     AGVFASAFVIGRSASKPVEQARVRQLEFTADASHELRTPLTVIEAEVGLALVTERSAG
                     GYRDALQRVSGETHRLRKIVEDMLWLARFDSEPPPPRTELVDVTTVVQQCVDRFEPVV
                     KSRNIKLSIEVAADAPALIAAAPEWLDRLIGVLLDNATRYAKSAGHVVVRVDGSSGHV
                     ALTVSDDGPGIPLAERQRLFDRFRRIEDNGGEGAGLGLAIADAVVRSTQGRWAVGEST
                     LGGARMTVIWPRPRSQSATRLPSLRS"
     gene            complement(3454..4122)
                     /locus_tag="VGD55_01315"
     CDS             complement(3454..4122)
                     /locus_tag="VGD55_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869011.1"
                     /translation="MRVLLAEDDEALRGVLVRGLTENGYVVDAVADGEAALAYLATYE
                     YEVVVLDWRMPRMSGYDALAAIRARRIAVPVLMLTALDRPADRIAGLDRGADDYLVKP
                     FDFGELLARLRALQRRPAVMKEPVLRCGDLSFDPAAREAFVDDRALAVTATELAILEL
                     LIRRSPAVVTRQSIALNVWPVEADGIGSNTIDVHLARLRGKLVGSHTRVETIRGVGYR
                     LLPS"
     gene            4257..4838
                     /locus_tag="VGD55_01320"
     CDS             4257..4838
                     /locus_tag="VGD55_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869012.1"
                     /translation="MSSDSGAEGNARLTGSAAAVLLILLAAEGATLPALHALLRPHVF
                     IGFALIPPVALKIGSTLYRFGRYYRRAPNYRRKGPPPTVLRILGPVVILTTILVLGSG
                     VALLFLNANWQQHMLTLHKLSFVVWFGAMTVHVLGHILETAQLAPRDWNPRSTHSLPG
                     SRIRRWALGASVLAGGLLGAWGLGHVGGWHGFR"
     gene            complement(4893..4969)
                     /locus_tag="VGD55_01325"
     tRNA            complement(4893..4969)
                     /locus_tag="VGD55_01325"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4933..4935),aa:Met,seq:cat)
     gene            complement(5013..>5374)
                     /locus_tag="VGD55_01330"
     CDS             complement(5013..>5374)
                     /locus_tag="VGD55_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0869013.1"
                     /translation="PLLATSMQMLAGGAVLLVAAAVTGEFGQFDPAHVSAASWLALAY
                     LIGPGSIIAFSAYVVAVRRLPTATVATYAYVNPIIAVILGTSLLGEALTASMLAGGAL
                     IVVAVVLVVRSQAAARR"
BASE COUNT         1008 a         1805 c         1720 g          841 t
ORIGIN      
        1 agcacaggat ttctcgtctg cgccgcctcg aggcaaggac cttcacctgc ggtgaattgc
       61 aacccgtcga gcaatctcgc ggctgggtca ctagcaccga acgtgccgcg actttctcct
      121 tcgtcgatca gagagactgc ggcagcatcg acaccgagcc acggcaagca cgcgatgcac
      181 aaccggtctg ctgcgggcgc gcctcgctca cccgcgacga gaacgtcggc gagcacggcc
      241 ctcacactcg cgcttccggg cacaccggca gccctcccgg cgatctacga tgcgatgcgc
      301 gcatcccgac gtcacggagg catgggccaa tagtcgtccg agcttggccg gacgacgaga
      361 ggcggccgtt catcgccggt cggcggattc cgtagtcggg tcgacacaga tgcagcgtcg
      421 tcgcagttcg ttcgacacgg tcgcccggca ataacgcaca ccatgcgcgt cgtgccgctc
      481 aagtggatgc ccgcaggtgc agagctcggt cgtagggtct tccggcatct cgccttgacc
      541 gtcgtcgacg gcaaccggcg acagctgcgc attttggctg gaatcgtcaa tcgagcgctc
      601 gatcacgtcg ctcgccgcct taaaggtcat cagcagacgc ggcgaggggg cgcaaggtcc
      661 agacagaccc aagcacgctg aatctgccca gcatgacacg aagtcaccac gaaagatatg
      721 gcgtttgggc tgttataggt cagatagggc tggcacaggc acactccacg caagacctac
      781 cgcgcgtaca gctgcccgtc gggtccctcc gcgaccgtaa tgcgtccaag gccgcgactt
      841 gctggcccga cctcgacggc accggtgctg ccgttgaact gcgagccgtg gcacggacac
      901 atgaatgtca agctcccctc gtcataggcg acagtgcagc cggcgtgcgg gcacaccgcg
      961 tcgaacccga ggaacgtgcc tgcgcggggc tgcacgacga tcgctggatc accggtcgca
     1021 gggtcctgga aggtggccga ccctttgacc ggcaccgcac ttgcggcgcc aatcttggta
     1081 ccagtgggct tcgtgccagc cgacttggta ccggtggtgg cggtggcctt cgaggtgggc
     1141 gtcggtgtgc cggaagagct cggggtgccg gtatcagtcg gggcactggt cgcggcctcg
     1201 gacggggccg ccgttgctgg ggtcgatcct gcgagcgacg gcacagccgc cgaagcggac
     1261 gacgatccat gcacgagtcg agccacgccg gcgatcgcag ccgtgccaag aaccgccacg
     1321 gcgccggcac ctagcgtgag cgcacctact aggcgacgcc gatcgacgtc gagcttcgcc
     1381 gctgccgcgc cacgcgccgg cttcgacaac tcgcgcgcga gatacgggca gccggccggt
     1441 tggcacgcag cgttgtgctg agcttcgcag cgaccgccgt gataatgccc gcagatcgac
     1501 tgcacggtcg cgaacggcaa ctgcacaggc aactgcgcag gccgtagatg ccgcgcggca
     1561 atgaggttcg acgtcagcgc atcgagggag agttgcccac caccggcgat gatgagcggc
     1621 atccaagcga acacgaacac gatgtcgctg ccggtgtagt acgggttcgt atgaaaactc
     1681 accgcgagga agaggttgag cgagagcaac aagccgccga ttgccgcgat tcgcgtgagg
     1741 agcccgagca atgcgccgac accgaccgcg atctcaccca gggcgatcac cgcaccgaga
     1801 agtgccgcgt gatgcgacaa cggtgtgatc agcgcgtgga tcggactgcg acgtgcggca
     1861 ccggccattt gcgcctggat cgacgccggg ttcgacgcct tgaagaacgc cgggttggcg
     1921 agcttctgca agcccgcaaa gcaaaaggtg aatccgagaa atgcgcgcaa cggcacgagc
     1981 gcccacccgg ccaggtctcg cgcaacaggt ggcggagcca accacaacca gcggcccctg
     2041 tccgtcgcgg gcagcgcagg agcggtcgga ggttgccgac gaccaccgct cataaaacgc
     2101 tcccgcctga agaagatcgc gtcagtcctg cgtccagcat gaaggatctt ccttgcgatt
     2161 cgctgaaagg gtgtcgcgcg cacttatgag cgcagcgacg gcaaccttgt cgccgactga
     2221 ctgcggggtc tgggccagat caccgtcatg cgggcgccgc ccagcgtcga ctcccccaca
     2281 gcccagcgac cctgcgtgct gcggacaacc gcgtcggcaa tcgcaagccc gagaccggca
     2341 ccttcaccac cgttgtcttc gatgcggcgg aaccgatcga acagccgttg ccgttcagcg
     2401 agtggaatcc ctggcccgtc gtcggacacg gtcagtgcca cgtgccccga cgaaccgtcg
     2461 acccgaacca ccacgtggcc cgccgacttg gcgtacctgg tcgcattgtc gagcagcacg
     2521 ccgatgagtc gatcgagcca ctcaggcgct gcggcgatga gcgccggagc atcagcagcc
     2581 acctcgatgc tgagcttgat gttcctcgac ttcaccacag gctcgaaccg atcgacgcat
     2641 tgctgcacga cggttgtgac atcgacgagt tcggtgcgcg gcggcggcgg ctcggaatcg
     2701 aaccgagcaa gccacagcat gtcctcgaca atcttgcgca gccgatgtgt ctcaccggac
     2761 acgcgctgaa gcgcgtcgcg gtagccgccg gcacttcgct cggtcaccag ggcgagtccg
     2821 acttccgctt cgatcaccgt gagcggggtg cgcagttcgt gggacgcgtc cgccgtgaac
     2881 tccaattggc ggacccgtgc ctgctcgacc ggtttggacg ccgaccgccc gatgacaaac
     2941 gcacttgcga acactccggc gagcaagagc ggcgacacgg caaacgcggc catgagcagc
     3001 acgctccgga cgtggtgctg ctgaacgaga ctctcagccg cgtacaaagt cgagccgtcg
     3061 ggttgtcgag cgtggcgaaa tcgaaatgcg tgaccggcaa tcgacacgct ccggtatcgg
     3121 gcttgcgacc cgttcaccag cgtgctcggc agcgccggtg caccagcgct cgtcgctaca
     3181 acagcgccgc cgcgcacttc ccagacatag gcgggtgggt cgtcggcatc gtggattgac
     3241 gctttagcca agaactcagc caccgaaaca ttgcgagtct gacgcaggag ttcggttagt
     3301 cggttatcgg tttcatgcag aagcctgttc gaaaccaacg ccaggatgcc ggccacgatc
     3361 acgatgtagc acagggcaac cgcaagagtc gctgccgccg ccacacgcaa tgcggggcgc
     3421 aggggcaccg tcgttttcat ctgctgggca tcatcacgag ggcagcaatc ggtacccgac
     3481 gccccgtatg gtctcgacgc gggtatgcga gcccacgagc ttcccgcgca gcctcgcgag
     3541 gtggacgtcg atggtgttcg agccgatgcc gtcggcctcg accggccaca cattcaacgc
     3601 aatcgactgg cgcgtcacca cggcaggact tcgacgtatc aggagctcga ggatcgcgag
     3661 ttcggtcgct gtcacggcca acgcgcggtc gtcgacgaac gcctctcttg ccgccggatc
     3721 gaagctcaag tcgccgcaac gcaagaccgg ctctttcatc acggcgggtc tgcgctggag
     3781 cgcacgcagg cgcgcgagca gctcgccgaa gtcgaacggc ttcacgaggt agtcatcggc
     3841 gccgcgatcc aatccagcga tccgatctgc gggccggtca agcgcggtca gcatcaatac
     3901 cgggacggcg atccgccgcg ctctgatcgc ggcgagcgcg tcgtagccac tcatccgcgg
     3961 catccgccag tcgagcacga ccacctcgta ctcgtaggtg gccagatagg ccagagctgc
     4021 ctcgccatcg gcaacagcgt cgacgacgta gccgttctcg gtcagcccac gaacgagcac
     4081 gccgcgaagc gcttcgtcat cttcagctag cagcacgcgc acagatcatc ctctccggcg
     4141 caaccttacg gacgactgac gactgctgcc gactactccc ggaccgtcgc gatccagcca
     4201 ccctgcatga ctgacgcttc atcgaaccgt aaggcactcg ctcgtagcgt cgtccgatga
     4261 gcagcgacag cggcgcagag ggaaatgccc gtctcaccgg gtcagcagcc gccgtccttc
     4321 tcatcttgct tgcggccgag ggcgcgacgc tacccgccct tcacgcgctg ctgcgtcccc
     4381 acgtctttat cggctttgcg cttatcccgc ctgtggcact caagatcggc agcaccctgt
     4441 acaggttcgg caggtactac aggcgagcgc cgaactaccg gcgcaaaggc ccgccgccga
     4501 ccgtgctgcg aatccttggc cccgtcgtga tcctgacgac gatcctcgta ctcggcagtg
     4561 gcgttgccct gctgttcctc aatgcaaact ggcagcaaca catgctgacg ttgcacaagc
     4621 tcagcttcgt cgtctggttc ggcgcaatga cggtccacgt cctgggccac atcctcgaga
     4681 ccgcgcaact ggctccccgc gactggaacc cgaggtcgac acactcgcta ccgggatctc
     4741 gtatccgacg ctgggcactc ggcgcaagcg tccttgcggg cgggttgcta ggagcgtggg
     4801 gtctcggaca cgtgggaggg tggcacgggt tccgatagag gtggctgaaa acatctcggg
     4861 acacccacgt atgtggatgt cccgagatcg tttggtagcg ggggcaggat ttgaacctgc
     4921 gacctctggg ttatgagccc agcgagctac cgaactgctc caccccgcgt cggtgtgcct
     4981 tcactttatg gccaaccgga ggcagaggca aatcagcgcc gggcggccgc ctgggaccgc
     5041 acgacgagca ccacggcgac gacgatgagc gcccctccgg ccagcatcga cgccgtcagg
     5101 gcctcgccca gcagggacgt gccgaggatc acggcgatga tcgggttgac gtaggcgtac
     5161 gtggcgacgg tcgccgtggg caggcggcgc acggcgacca cataggcgct gaacgcgatg
     5221 atcgagcccg gcccgatgag ataggccagg gccagccacg acgcagctga cacgtgcgcc
     5281 gggtcgaact ggccgaactc cccggtcacg gcggccgcca cgagcagcac cgccccgccg
     5341 gcgagcatct gcatcgaggt ggccaggagc gggc
//
LOCUS       DASWVF010000076         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_52953, whole genome shotgun sequence.
ACCESSION   DASWVF010000076 DASWVF010000000
VERSION     DASWVF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52953"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VGD55_01335"
     CDS             <1..1170
                     /locus_tag="VGD55_01335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0869014.1"
                     /translation="PFEGVVSELLVAEVTMIEVGTPIIIIDVAPGAQPPLEVAAAAAS
                     RTANLVGYGPRADSAVRRRPRAVPVVAAPRPAPTWPSVAAAVAVPDEPEVIDGESVSG
                     VLVKPPIRKLARDLGIDVQTLHGTGPEGSITRSDVEAAADTSRAGAPARPLPRGGEER
                     IEIRGVRKATAQAMVQSVFTVPHVTEFTTIDMTRSMRAVTRLRELPEFVDVKVTPLLL
                     VAKAILLGLERYPMVNSTWDEPNGEIVVKHYVNLGIAAATPRGLVVPNIKNAEALSLA
                     QLARSINVLAVTAREGRTSVADMQRGTFTITNVGVFGIDTGTPIINPGEAAILAFGAI
                     REQPWVHRGKVKPRFVTTLALSFDHRIVDGELGSRFLRDVAMFLEDPASSLLARL"
     gene            1180..>1958
                     /locus_tag="VGD55_01340"
     CDS             1180..>1958
                     /locus_tag="VGD55_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HEY0869015.1"
                     /translation="MRVALLQLRIDDSESASARIARVAALVTEQVGADLVVLPELWVP
                     GAFAYRGYQASADEIFGTAVSAIADAAKSIRAFVAAGTFIERDGEQLHNTAVLLSPRG
                     DLLHTYRKLHLFGFNHGEALVLTAGDDWAITAIDGARVGITTCYDLRFPELYRLFVNQ
                     GAEMFVIPTGWPSARLEHWQVLTRARALENQVFLIGCNQVGTQEGVELAGHSVVIDPW
                     GRVIAEAGTEEEVLTVDIDLALVAKVRAEFPVLGDRRLGLPL"
BASE COUNT          325 a          646 c          648 g          339 t
ORIGIN      
        1 ccgttcgaag gcgtcgtgag cgagctgctc gtcgccgagg tcacgatgat cgaggtcggc
       61 actccgatca tcatcatcga cgtcgcaccg ggcgcgcagc cgccgctcga ggtcgccgca
      121 gccgccgcga gtcgcactgc caacctcgtc ggctacggcc cgcgagccga ctcggcggtc
      181 cgacgcaggc cgcgcgcagt accggtcgtc gcggcgccgc gaccggcacc aacctggccg
      241 tcggtcgcgg ctgccgttgc ggtgcccgac gagcccgagg tcatcgacgg agaaagcgtg
      301 agcggcgtgc tcgtaaagcc gccgatccgc aagctggcgc gcgatctcgg catcgacgtt
      361 cagacattgc acggcaccgg acccgaaggt tcgatcactc ggtcggacgt cgaggcagcg
      421 gccgacacct caagggctgg cgcacctgct cgtccgctac cgcgcggcgg cgaagagcgg
      481 atcgagatcc gcggggtccg caaggcgacc gctcaggcca tggtgcagag cgtcttcacc
      541 gtgccgcacg tcaccgagtt caccaccatc gacatgacgc gctctatgag ggcggtcacg
      601 cggttgcgcg aactgcctga gttcgtcgac gtgaaagtga cgccgctgct gctcgtcgcc
      661 aaggcaatcc tgctcgggct cgagcgctac ccgatggtga actcgacatg ggacgagccg
      721 aacggcgaga tcgtcgtgaa gcactacgtc aacctcggca ttgcggcggc gacacctcgc
      781 ggtctggtcg tgccgaacat caagaacgcc gaagcgctct cactcgcgca gctcgctcgg
      841 tcgatcaacg tgttggccgt cacggcgcga gagggccgca cgtcggtcgc cgatatgcaa
      901 cgcggcacct tcacgatcac caacgtcggc gttttcggca tcgacaccgg caccccgatc
      961 atcaaccccg gcgaagccgc gatcctcgcg ttcggcgcga tccgtgagca gccctgggtg
     1021 caccgtggga aggtcaagcc acgcttcgtc acgacccttg cgttgtcgtt cgaccaccgc
     1081 atcgtcgacg gcgaactcgg ctcgcgcttc ctgcgtgatg tggcgatgtt cctcgaagat
     1141 ccggcgtcgt cactgctcgc ccggttgtag gagttttcct tgcgggtagc gcttctgcag
     1201 ctacggatcg acgacagcga atcggcgtcc gcccgtattg ctcgtgtcgc agcactcgtc
     1261 acagagcaag tcggtgccga cctcgtcgtg ctgccggagt tgtgggtgcc gggtgcattt
     1321 gcgtaccgcg gataccaggc gtcagccgac gaaatttttg gcaccgcagt ctcggcgatc
     1381 gccgatgcgg ccaagtcgat cagagcgttt gtggctgccg gaacgttcat cgaacgcgac
     1441 ggcgagcagc tccataacac cgcagtgctg ctctctccac gtggcgacct gttgcacacc
     1501 taccgaaagc ttcacctgtt cggcttcaat cacggcgagg cgttggtgct gacggccggc
     1561 gacgactggg cgatcacggc catcgacggg gcgcgggtcg gcatcaccac gtgttacgac
     1621 ctgcggttcc ccgagctgta tcgcctgttc gtcaaccagg gcgccgagat gttcgtgatc
     1681 ccgaccgggt ggccgtcggc tcggctcgaa cactggcagg tgctcacgag ggcgcgtgcg
     1741 ttggagaacc aggtcttcct gatcggctgc aatcaggtcg gaacccaaga aggtgtcgag
     1801 cttgcggggc acagcgtcgt catcgatccg tggggacggg tcatcgccga ggcaggcacc
     1861 gaggaggagg tgctgacggt cgacatcgat ctcgcactgg tcgcgaaggt ccgtgcagag
     1921 tttccggtgc tcggcgaccg caggttgggc ctgccgct
//
LOCUS       DASWVF010000077         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_384252, whole genome shotgun sequence.
ACCESSION   DASWVF010000077 DASWVF010000000
VERSION     DASWVF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384252"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..>1800
                     /locus_tag="VGD55_01345"
     CDS             185..>1800
                     /locus_tag="VGD55_01345"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEY0869016.1"
                     /translation="MNAPHFDETSGLADFLVVGAGPVGLVTAILLGREGWHVVVVEKW
                     PSRYPLPRACTIDHEALRILQAAGVMREHADLFEPSRGERGGYQIRNGEGEILRAINW
                     NRQAESGWANVNGFYQPDLEAVLESMAQALPSVELRRGWSAAAIDQDEDSITLTVFRT
                     NDHSQHDSLRGRWLVGADGANSVIRELAGIESVDTGFEADWLVVDYQPLDHRQWDAFV
                     TQYCDPVQPATAVNSGPGRRRFEFMRRADISADELGRMETAWKLMEPWGVTSETARLE
                     RHAVYTFRGRWARQWRRGHIFLAGDSAHLMPPFLGQGLCSGLRDARSLAWRLSLVESG
                     RAHESILDSYGTERGGHVQEIITEAVAIGKVICELDPLRAAARDAEMRAELNDPEAVT
                     VEPPHPRLGRPSITQAGDDNAGRLSIQARVEANGSVGLFDDVVGGTWQLIGLDAEPMV
                     DVSAELRTWFEDLGGTATALAVDGGVRDIDGRYRAWFEAHGCSLLLARPDFYIYGTGA
                     AHDAARLLGALRNALGVAVTDTNELIEKGT"
BASE COUNT          318 a          536 c          601 g          345 t
ORIGIN      
        1 ccgtccagcc ctctgcggac ggcgagatcc agcgttctga ccacgttcga gctgggcacc
       61 agtacttttg aggcgggtta ccaggccgcg ccgccattcg agccgctgtc ggccgacatc
      121 gtcttcgaga aggatgtcgc gatcacgttg cgagacgggg tcggaggtag gagatgacag
      181 acctatgaat gcaccacact ttgacgagac gtcaggcctg gccgatttcc tcgtcgtggg
      241 cgcgggcccg gttggtctcg tgacggcaat tctcctcgga cgtgagggat ggcacgtggt
      301 agtcgtcgag aaatggccgt cgcgctatcc gctgccacgg gcatgtacga tcgaccacga
      361 ggctcttcgt atcctgcagg cggccggggt gatgcgtgag catgccgatt tgttcgaacc
      421 gtcgcgcgga gaacggggtg gctaccagat tcgcaatggt gaaggtgaga tccttcgcgc
      481 gatcaactgg aaccgacagg cagagtccgg ctgggcgaac gtcaacgggt tctatcagcc
      541 cgacctcgag gcggtactcg agtcgatggc acaggccctg ccatcggtgg agctgcgtcg
      601 aggatggtct gccgctgcca tcgaccagga tgaggactcg atcacgctga cagtgttccg
      661 caccaacgat cattcacagc atgattcgtt gcggggtcgc tggctcgtcg gtgccgacgg
      721 cgctaatagc gtcattcgcg aactcgctgg aatcgaaagc gtcgacacgg gattcgaggc
      781 ggactggctc gtcgtcgatt atcaaccgct cgatcaccga cagtgggatg cgtttgtcac
      841 gcagtattgc gatccggtcc agccggcgac ggcggtcaac agcggccctg gtcggcgacg
      901 cttcgagttc atgcgtcgag cggatatctc ggccgacgag ctaggacgaa tggagacggc
      961 gtggaaactg atggagccat ggggggtgac atctgaaacc gcgcgacttg agcgccatgc
     1021 cgtctatacc ttccgcggcc ggtgggcgcg gcagtggcgc cggggccaca tttttctcgc
     1081 tggagattcc gcccacttga tgccaccgtt tctcgggcag ggcctgtgct cgggacttcg
     1141 tgatgcgcgt tcgctcgcct ggcggttgtc cctggtcgag tccggtcgag cgcacgagtc
     1201 gattctcgac tcgtacggca cagagcgtgg cggtcatgtg caagagatca ttacggaagc
     1261 tgtcgcaatc ggcaaggtga tctgcgaact cgacccactc agagcagccg cgcgggatgc
     1321 agagatgcgc gccgagctga atgatcccga ggcagtcacc gtcgagccgc cccacccgcg
     1381 actcggtcgg ccctcgatca cccaggcagg cgacgacaac gctggccggc tgtccattca
     1441 ggctcgggtc gaagcgaacg ggagcgtcgg gctcttcgac gatgtcgtgg gtggcacctg
     1501 gcagctcatc gggcttgatg cagaaccgat ggtggacgtt tcggcggaac tccgcacgtg
     1561 gtttgaagac ctcggcggca cggctaccgc gctcgccgtc gacggcgggg ttcgcgacat
     1621 tgatggccgc taccgtgcat ggttcgaagc gcacggctgc agtctcctgc tcgcgcgacc
     1681 cgacttctac atctatggca ctggcgctgc gcacgatgcc gcgcgccttc tcggcgctct
     1741 gcgcaacgct ctgggcgtcg ctgttacgga cacgaatgaa cttatcgaga aaggtacatt
//
LOCUS       DASWVF010000078         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_333175, whole genome shotgun sequence.
ACCESSION   DASWVF010000078 DASWVF010000000
VERSION     DASWVF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333175"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGD55_01350"
     CDS             <1..504
                     /locus_tag="VGD55_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869017.1"
                     /translation="RPARDDVAMGLRKPRRLRDVHPTRRGLILAWFAFAVTFGVLRVI
                     TWSIHAHVGHFANVTAGSVHLHHYLWGMLLVVIVAFLGLVERSPTWRTAMGLAFGIGL
                     ALIVDELALLIDLKDVYWSGAGGVSIAVALVLIAVTGGLIALTRAPGRDDDINQKAQP
                     TTRTAPE"
     gene            complement(474..>1559)
                     /locus_tag="VGD55_01355"
     CDS             complement(474..>1559)
                     /locus_tag="VGD55_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0869018.1"
                     /translation="ALLPAATSLAAQPKTVAQAQAQLDDLNNQAEIASEQYNGAQLAL
                     TGAQQAASAATAAADRARSDVTTEQAKVGVLAAQSYETGGLRQGLAVVLSSGNPAQTM
                     ARMSMLEQLGTQQSAVLADVRSADLRYQQSVEASAQATATATQLTAKLAQEQAQINAA
                     LAQSQKVLAQLTAAQRAQLLAAQQAKAAADQVRAAAALASYRKAQATQAAQAVSRSLA
                     RAAAAKAQSVQNAAAIQALPQSSRGSTVAQRAVSAAMSRLGDRYVFGATGPTRFDCSG
                     LVAWAYRQAGISTTHYTGTLYNDYRDIPESQLKPGDLVFFYRDHHHVGIYIGNGLMIN
                     APHTGDVVRIASVMGHGSYSGAVRVVG"
BASE COUNT          223 a          497 c          531 g          308 t
ORIGIN      
        1 cgtccggcgc gcgatgatgt ggcgatggga cttcggaagc cgcgccggct ccgtgacgtg
       61 catccgacca ggcggggact gatcctcgcc tggttcgcgt tcgcggtgac cttcggtgtg
      121 ctgcgcgtga tcacctggtc gatccacgcg cacgtgggcc atttcgcgaa tgtcaccgcc
      181 ggctccgtgc atctgcacca ctacctgtgg ggcatgttgc tcgtggtcat cgtcgcgttt
      241 ctcggcctgg tcgaacggtc accaacctgg cgcacggcga tgggcctagc gtttgggatc
      301 gggctggctc ttatcgtcga cgaactcgcg ttgctcatcg acctgaagga cgtctactgg
      361 tcgggcgcag gtggtgtgag catcgcggtc gcgctggtgt tgatcgcagt caccggaggg
      421 ctaatagccc tcactcgcgc gccgggtcgt gacgatgaca taaaccagaa agctcagccg
      481 acgacgcgaa cggctcccga ataagaaccg tgacccatca ccgacgcgat gcgcacgacg
      541 tcaccggtat gcggggcatt gatcatcagt ccgttgccga tgtagatgcc gacgtggtga
      601 tggtcgcggt agaagaacac caggtcgccg ggcttcaact ggctttccgg aatgtcgcgg
      661 tagtcgttgt acagcgtgcc ggtgtagtgg gtcgtgctaa tgccggcctg ccggtaggcc
      721 catgcgacga gccccgaaca gtcgaacctc gtcgggccgg ttgctccgaa cacgtaccgg
      781 tcgccgaggc gactcatcgc ggccgagact gcgcgttgag caacagtcga gccgcgtgaa
      841 ctttgcggca acgcctgtat cgccgcggcg ttctgcaccg actgggcctt cgcggcagca
      901 gcgcgcgcaa gactgcggct gacagcctgt gctgcctgcg tcgcttgggc cttgcggtac
      961 gacgcgagtg cggccgctgc gcgaacttgg tcggccgcgg cttttgcctg ctgggccgcg
     1021 agcagttgcg cgcgctgcgc tgctgtcaac tgcgcgagca ccttctggct ctgcgcgagt
     1081 gccgcgttga tctgcgcctg ctcttgcgcg agcttggccg tcagttgcgt ggcggttgcc
     1141 gtggcctgcg ccgaggcctc gaccgactgc tgataccgaa gatctgcaga ccggacgtcg
     1201 gccagtaccg ccgactgttg ggtaccgagt tgttcgagca tcgacatccg cgccatcgtc
     1261 tgcgcgggat tgccgctgct cagtacgacc gcgagccctt gccgaagccc gcccgtctca
     1321 tacgactgcg ccgcgagcac gccgaccttc gcttgctctg tcgtcacgtc ggagcgggcc
     1381 cggtcggcgg cggctgttgc cgcactcgcc gcctgctgag cccctgtcag ggcgagctga
     1441 gcaccgttgt actgctcgga ggcaatttcg gcctggttgt tcaggtcatc gagttgcgcc
     1501 tgcgcttgtg cgacggtctt gggctgcgca gcaaggctgg tggcggccgg caacagggc
//
LOCUS       DASWVF010000079         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_435256, whole genome shotgun sequence.
ACCESSION   DASWVF010000079 DASWVF010000000
VERSION     DASWVF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435256"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /gene="paaN"
                     /locus_tag="VGD55_01360"
     CDS             complement(<1..1177)
                     /gene="paaN"
                     /locus_tag="VGD55_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEY0869019.1"
                     /translation="MRNPAALVATHAELLAQARAAVRARTYFSAFAESPSTRIWGEGA
                     PIDGRAAFDGLLHQTFPLETPGARGLIAPEKPPYGVPLDISYPHLHPDGVDEVVSAAK
                     KAMPAWRDAGPDLRTAIGIEILTRINARSFEFAHAVMHTTGQAFVMAFQAGGAHAHDR
                     GLEAIAYAYDAMAQVPASVSWEKPRGKGDPQRLAKTYTIVPRGIAVVIGCSTFPTWNS
                     YPGLFASLITGNPVIVKPHPNAVLPLALTVSVARDVLTEYGFDPNLVTLAVDGPGETI
                     ASTLATHPDVRVIDYTGSTSYGQWLEANALQARVFTEKAGVNCVVLDSTDDLNGLAAN
                     LAYSLSLYSGQMCTTPQTIFVPRDGIDTDRGHANMADVVSALSNALDELLGDPARAVN
                     "
     gene            complement(1210..>1725)
                     /locus_tag="VGD55_01365"
     CDS             complement(1210..>1725)
                     /locus_tag="VGD55_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0869020.1"
                     /translation="GLSEYDVRRPMTPTATNLLGLVKHLANVEIGYFGDTFGRPFEGP
                     LGWPDGSDEPDDPTADLWATADESPQYVIGLYRAAWVHADVTIETLQLEAVGRVPWWP
                     AERAEVSLHKILVHVIAETNRHAGHADIVRELIDGTTGLREGNHNMADGDQAWWEEYR
                     NRLEQLARHAR"
BASE COUNT          287 a          547 c          573 g          318 t
ORIGIN      
        1 tgttcaccgc gcgcgcaggg tcaccaagca actcgtccag tgcgttgctc agtgcgctga
       61 caacgtcggc catgttggca tgtccgcgat cggtgtcgat gccgtcacga gggacgaaga
      121 tggtctgagg agttgtgcac atctgaccgc tgtagagcga cagcgagtac gcaaggttgg
      181 cagccagccc gttgagatcg tcggtggagt caaggacgac gcagttcact ccagccttct
      241 cggtgaacac cctggcctgt aacgcattcg cttcgagcca ttggccgtac gacgtcgacc
      301 ccgtgtagtc gatgacgcgc acgtcagggt gcgttgccag ggtggaagcg atggtttcac
      361 cagggccatc aacggcgagg gtaaccaggt tcggatcgaa tccgtactca gtcagcacat
      421 cgcgggcgac actgaccgtc aacgccagtg ggagtacggc gttcggatgc ggcttcacga
      481 tgacgggatt acccgtgatc aggctggcga acaggcccgg ataactgttc caggtcggga
      541 atgtcgaaca gccgatgacg acggcgatcc cgcgaggcac gatcgtgtat gtcttggcga
      601 gtcgctgcgg gtcacctttg ccgcgaggct tctcccaact taccgacgca ggcacctgcg
      661 ccatcgcgtc gtaggcgtag gcgatcgcct cgagcccgcg atcgtgcgcg tgtgcgccgc
      721 cggcctggaa cgccatgacg aacgcctgac cggtggtgtg catcacggcg tgcgcgaact
      781 cgaagctgcg tgcgttgatc cgagtcaaga tttcgatacc gatcgcggtc cgaaggtcgg
      841 gccctgcatc gcgccatgcc ggcatcgcct tcttggctgc gctcacgact tcgtcgacac
      901 cgtcgggatg caggtgcgga tagctgatgt cgagcgggac cccgtacggc ggcttctcag
      961 gagcgatcaa tccgcgcgcg ccgggcgtct cgagtggaaa cgtctggtgt agcagcccgt
     1021 cgaacgcggc cctgccgtct atgggtgccc cctcacccca aatccgggtg gacggcgatt
     1081 cggcgaaggc cgagaagtag gtgcgggctc gcacggccgc gcgcgcctgc gccagcagtt
     1141 cggcatgggt cgcgacaagt gcggccggat tgcgcatggt gcaagtaaac cgaacgttcg
     1201 gtcgacgcgc tagcgggcgt ggcgggcgag ctgttcgagc cggtttcggt actcctccca
     1261 ccacgcctgg tcgccgtctg ccatgttgtg gttgccctca cgcaagccgg tggtcccgtc
     1321 gatgagttcg cgcacaatgt cggcgtgtcc ggcgtgccgg ttcgtctcgg cgatcacgtg
     1381 caccaagatc ttgtgcaggc tgacttctgc gcgctccgcc ggccaccagg gcacccggcc
     1441 gaccgcttcc agttgcaggg tctcgatcgt cacatccgca tgcacccacg ccgcgcggta
     1501 caagccgatg acgtactgcg gcgactcgtc ggccgtggcc caaaggtcgg cggtcggatc
     1561 gtccggctcg tcactaccgt ccggccatcc gagcgggcct tcgaacggcc tgccgaatgt
     1621 gtcgccgaag tacccgatct cgacgttcgc cagatgtttg accaggccga gcaggttggt
     1681 tgcggtcggc gtcatcggac gccgcacgtc gtactccgac aggcc
//
LOCUS       DASWVF010000080         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_180463, whole genome shotgun sequence.
ACCESSION   DASWVF010000080 DASWVF010000000
VERSION     DASWVF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180463"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGD55_01370"
     CDS             <1..444
                     /locus_tag="VGD55_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869021.1"
                     /translation="ALPGDAINLLGNEFAAGLDSVPAGHGTAKFTVITQPTDHAKGLA
                     TAQKLASSIDQGTAAPTVRTAGSDVIISNDPQATGSLGDEPAFKAAMSGVPDQVVGAV
                     YVDLAGIWAAKPGTVPSDLTHLTGLGGYEAIDGNDLVFDARLTVG"
     gene            539..>3097
                     /locus_tag="VGD55_01375"
     CDS             539..>3097
                     /locus_tag="VGD55_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEY0869022.1"
                     /translation="MSTAQADLVSWLRAQSDDDLVRLLAARPDLLHPVPFDIGVLAAR
                     AGGHASVEIALDRLDIGALQVIEGLTLSADPTNLSSLATLLDVVPERIAATVERLRQA
                     ALVWGSDDALRLLPAVRDLMHSPAELGPPARALLENVPRSRLATLLRDLGLPDVGDPS
                     LDASAALAAVDSLASFIEDSDQLAALLERVPAPAREVIDLLAAGPPAGRVPAARRPVD
                     AATADSPVRWLLAHALLVAVDDFTVVLPREIAIHLRGGHAFRALELDPPQGESAVHSP
                     ADVDAAAAGQAFTFVRLVEALLERWGVDAPGVLRAGGLGVRELRRTARELDVEERVAA
                     VLIEVSHAAGLIAPDGESASSWLPTTGYDVWLARSVADRWTLLATAWLATTRVPGLVG
                     QRDQGRLSMAPSTPPPTLPSTPSEGVERPTSGANALGGDIDRALAPIVRREMLAILSQ
                     APVGSAPTMSSIAESLRWRWPRRGGRLRDDLVAWSHDEAELLGLTGRGALSSFGRILC
                     ADASPTDTSAADSQPAEPAPDPAVQAAVALAALLPQPLDHVLLQADLTAVAPGPLESE
                     LARELGLAADIESTGGATVFRFTDASIRRALDAGRSASDLHALLTARSRTPVPQPLSY
                     LIDDVARRHGRIRIGVAGAYVRCDDDAVLSEVLADRRVESLRLRRLAPSVLVSPLAPE
                     RVAERLREVGYAPAAESPDGALLLRRPDARRAPSRPRQLRRRPELAASAGSVVTLAVK
                     AIRGGDRAVTAARGLVVGGSPSDVLPHSASSETLSVLQDALTSGRAVWIGYLNAQGQA
                     TNRIVEPQRLSGGYLTAFDYRRDEPRTFAIHRITGVAELTDVVAELDAELDGPPA"
BASE COUNT          440 a         1123 c         1034 g          500 t
ORIGIN      
        1 gcgctacccg gcgatgcgat caacctgctc ggcaacgagt tcgcggcggg tctcgactcc
       61 gtacctgccg gccacggcac ggcgaagttc actgtgatca cccagcccac cgatcatgcc
      121 aagggtttgg cgaccgcgca gaagctcgca agctcgatcg atcaggggac ggcggcgccg
      181 acggtccgca cggccggctc cgacgtgatc atctccaacg acccgcaggc gaccggatcg
      241 ctcggtgacg aaccagcgtt caaagctgcg atgtcgggag tgccagatca ggtggtcggc
      301 gccgtctacg tcgatctcgc cggcatctgg gccgcgaagc ccggcacggt gccaagcgac
      361 ctcacccatc tcaccggtct gggcggctac gaggcgatcg acggcaacga cttggtgttc
      421 gacgcccggt tgactgtcgg ctgagcacgt ccagcttcgt ctgtgcacag aacaggaagc
      481 atccaccgca gcagtcgtca actcgtccaa tcgctggcag acgactaacg ttgaccggat
      541 gagcacagca caggccgatc tcgtcagctg gctgcgcgcg cagtccgacg acgacctggt
      601 gcgcctgctc gctgcgcgcc cagacctgct acatccggtg ccgttcgaca tcggcgtcct
      661 tgccgcgcgg gcggggggtc acgcatcggt cgagatcgcg ctcgatcgcc tcgacatcgg
      721 tgcgcttcaa gtcatcgaag gcctcacact ttcagctgac ccgaccaacc tgagctcgct
      781 cgccacactg ctcgacgtcg tgcccgagcg catcgcggcc acggtcgaac ggctccggca
      841 ggcggccctt gtctgggggt ctgacgacgc cctgcggcta ctgcccgccg tccgcgatct
      901 gatgcactca cccgccgagc tcgggccgcc agcccgcgcg ttgctcgaga acgttccgcg
      961 gtcgcggctc gccaccctgc tgcgcgatct cggactgccc gacgtcggcg atccgagtct
     1021 cgatgcgtcg gcggcgctcg cagcggtcga ttccctcgca agctttatcg aggactccga
     1081 tcagctcgcc gccttgctcg agcgagtgcc cgccccggct cgtgaggtca tcgacctgct
     1141 cgctgccggt ccgccggcag gccgggtgcc tgcggctcgg cgtccggtcg acgcggcgac
     1201 cgccgattcg ccggtgcggt ggctgctggc gcacgccctg ctcgtagcag tcgacgactt
     1261 cacggtggtc ctcccgcggg agatcgcgat tcacctgcgc ggcggccacg cgtttcgcgc
     1321 gctcgagctt gacccacctc agggtgagag cgcagtacac agtccggcgg acgtcgacgc
     1381 ggcggccgcc ggtcaagcgt tcaccttcgt gcggcttgtc gaagcgctac tcgagcgctg
     1441 gggcgtagat gctccagggg tcttgcgggc cggcggcctc ggcgtgaggg aactgcgacg
     1501 caccgcgcgc gaactcgatg tcgaagagcg cgtcgcggcc gtgcttatcg aggtctccca
     1561 cgccgctgga ttgatcgcgc ccgacggcga gagtgcgagc agctggctgc ccacgaccgg
     1621 gtatgacgtc tggctggcga gatcggtcgc cgaccgatgg accctgctcg ccactgcgtg
     1681 gctggcgacg acacgggtgc ccggccttgt cggccagcgc gaccagggcc ggctgtcgat
     1741 ggctccttcc acgcccccac caacgctccc atctactccg agcgaggggg tcgagcggcc
     1801 gacatcaggt gcaaacgcac tcggcggcga cattgatcgt gcgctcgcgc cgatcgttag
     1861 gcgcgagatg ctcgcgatcc tgtcgcaagc acccgtcggc tccgcgccca ccatgagctc
     1921 catcgccgaa tcgctgcgct ggcggtggcc gcgccgcggg ggcaggctgc gcgacgactt
     1981 ggttgcgtgg tcgcacgacg aggctgagct cctcgggttg acagggcggg gggcactgtc
     2041 gtcgttcggg cgcatcttgt gtgccgacgc gtcgcccacc gacacatcgg ccgccgactc
     2101 gcagccagcc gaacccgcgc ccgaccccgc ggtgcaggcg gccgtcgcac tcgccgcgct
     2161 gctcccgcag cccctcgacc acgtgttgct ccaagcagat ctgacagcgg tcgctcccgg
     2221 cccgctcgag agtgagctcg cgcgcgaact cggcttggct gccgacatcg aatcgacggg
     2281 cggcgccacc gtcttccggt tcaccgacgc atcgattcgg cgcgcgctcg acgccgggcg
     2341 cagcgcgtct gacctgcacg cattgctgac agcgcgatcg cgcacgcccg taccgcagcc
     2401 gctcagctac ctcatcgacg acgtcgcgcg caggcacggc cgaatccgca tcggcgttgc
     2461 cggtgcttat gtgcggtgcg acgacgacgc ggtgctgagc gaagtgctcg ccgatcgccg
     2521 cgtcgagagc ctgcgcctgc gccgactcgc gccgtcggtt ctggtcagcc cgctggcccc
     2581 cgagcgcgtc gccgagagat tgcgcgaggt cggctacgcg cccgctgctg aaagccccga
     2641 cggcgcgtta ctgcttcgcc gacctgatgc gcggcgtgcg ccctcgcgcc cccggcagtt
     2701 gcggcgccga cctgaactcg ccgcgtctgc gggttcggtc gtcaccctgg cggtcaaagc
     2761 cattcgtggc ggcgaccgcg cggtcacggc tgctcgcggc ctcgtggtcg gtggttcgcc
     2821 gtcggacgtg ctgccccact cggcgtccag cgagactttg tcggtcttgc aggacgcatt
     2881 gacctccggc cgggcagtgt ggatcggcta cctgaatgcg caaggccagg cgaccaaccg
     2941 catcgtcgaa ccgcagcggc tttcgggcgg gtacctgacc gcattcgact accgtcgcga
     3001 cgaaccccgc acgttcgcga tccaccgcat caccggggtg gccgagttga ccgacgttgt
     3061 ggccgagctt gatgccgaac tcgacggacc gcctgcc
//
LOCUS       DASWVF010000081        10152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_358831, whole genome shotgun sequence.
ACCESSION   DASWVF010000081 DASWVF010000000
VERSION     DASWVF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358831"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /gene="cimA"
                     /locus_tag="VGD55_01380"
     CDS             <1..1242
                     /gene="cimA"
                     /locus_tag="VGD55_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY0869023.1"
                     /translation="VLEALKTTLDENLAMIRDTVLHLRGEGRRVFVDAEHFFDGYKAN
                     PAYALEAVRVAAEAGADVIVLCDTNGGMLPTQLADVVHTVLQSSGARIGIHCHDDTGC
                     AVANTLAAVEAGVTHVQGVVNGYGERCGNANLVTVVANLELKLGRRVLPDDRVVELGR
                     VSHLVAEIANQPPRAHQPYVGLSAFAHKGGLHASAIKVSADLYQHIDPSLVGNDMRML
                     VSEMAGRASVEMKGRELGFDLSDDKDVVGRVVDRVKSLEAKGYMFEAADASFELLLRE
                     EINGEPLQYFTLESWRVIVEQRADGEVVSEATVKVQAKGERIVSTGEGNGPVNALDRA
                     LRLALERLYPQLAELELVDYKVRILEGAHGTGAITRVLIETSDGKTEWDTIGVDENLI
                     AASWQAMEDAYTYGLLRQHSS"
     gene            1261..2055
                     /locus_tag="VGD55_01385"
     CDS             1261..2055
                     /locus_tag="VGD55_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0869024.1"
                     /translation="MRIARFSYGDPDVAGYAFGAIGGTPGAEEVAVINGHPFGEITFS
                     GLRLPLAGVRLLPPVLPSKVVAIGKNYADHAREMGGEPPAEPVLFLKPSTSVIGPGAA
                     ITYPKLSQKVDFEGELAVVIGRLCREVPLERVPEVILGYTCANDVTARDLQKSDGQWA
                     RAKGFDTFCPLGPWIDTEVDPSDLAITTTVNGDVKQNARTSLLLHDVAELVVFVSAAM
                     TLLPGDVILTGTPAGVGPIVPGDEVAVTIEGIGTLTNTVIGTGVSR"
     gene            2052..3512
                     /gene="gltX"
                     /locus_tag="VGD55_01390"
     CDS             2052..3512
                     /gene="gltX"
                     /locus_tag="VGD55_01390"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0869025.1"
                     /translation="MSVRLRVAPSPTGDPHVGTAYMSLFNLAFARQQGGAFILRIEDT
                     DRGRYVEDSEQQIYDTLHWLGLDWDEGPDIGGPFAPYRQSERLGTYRPFFERLLADGH
                     AYYCWCSAERLTQMREDQQRRKQATGYDRLCYGKTREERALLPGFSETPVVRMLIPSD
                     VELTFVDLIRGRTSAPHPDDQVIVKGDGFPTYHLAVVVDDHLMGITHVVRGEEWISST
                     PKHILLYKWFGLEPPAFAHMPLLRNIDKSKISKRKNPAARLLWFREQGYLPEALRNFL
                     ALMGYSMPDGREIFSFDEMVESFDWTRVNPVGPVFDIDKLNWLNGQYLRALSVDDLAT
                     RLLPILQRADVIADPPAAGDVELLRAAAPLVHERMALLSEVIDMLGFLFVDDDDFEPD
                     ADAAAKNLGTDAAPALDAALSALASVGHWHADEIKAALEVALIGELGLKPRNAYAPLR
                     VAITGRTISPPLFESMELLGRERSMSRLQRAAASNA"
     gene            3592..3666
                     /locus_tag="VGD55_01395"
     tRNA            3592..3666
                     /locus_tag="VGD55_01395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3625..3627,aa:Gln,seq:ctg)
     gene            3709..3781
                     /locus_tag="VGD55_01400"
     tRNA            3709..3781
                     /locus_tag="VGD55_01400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3743..3745,aa:Glu,seq:ctc)
     gene            3816..4577
                     /locus_tag="VGD55_01405"
     CDS             3816..4577
                     /locus_tag="VGD55_01405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0869026.1"
                     /translation="MSANTPRIAVVTGGGSGIGAAVALALAADAWTVVVAGRRKEALD
                     ETVARGFASNGTLEAIPTDVTDEASVRGLFDAVVDAHGRVDLLFNNAGTGAPAVDIDA
                     LELDAWNQVVAVNLTGAFLCTREAFRVMRNQEPRGGRIINNGSISAHSPRPHSIAYTA
                     TKHAITGLTKSTSLDGRAFDIACGQIDIGNAATDMAGGAARGAAQADGSVRPEPLMDV
                     GEVGRAVCYMASLPLDANVASLIVLPPKMPFVGRG"
     gene            4582..5529
                     /locus_tag="VGD55_01410"
     CDS             4582..5529
                     /locus_tag="VGD55_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0869027.1"
                     /translation="MPNSELGATAWDHDRSIVHGPDPAIEVIDERFRSLVVGSEIVER
                     LWTGGRWLEGPVWFGDARHLLFSDIPNNRMLCWSEVTGAVTVFREPSNNSNGNTRDRQ
                     GRLVTCEHLTRRVTRTEYDGNITVLMDSYQGKPLNAPNDVTVHSDGSVWFTDPGWGIE
                     GNYEGVKAVKEIPRYVYRIGADGEGVPVITDMDRPNGICFSPDESLLYVVDVDQIRVY
                     DMDGTKPVNGRVFVAMKGSDGIRTDLDGNVWSAAGWGGPGFDSVHCYSPAGELLGQIN
                     LPETCANFCFGGAKRNRLFITASRSLYSVFVEAQGCQRP"
     gene            complement(5541..5864)
                     /locus_tag="VGD55_01415"
     CDS             complement(5541..5864)
                     /locus_tag="VGD55_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869028.1"
                     /translation="MRNLPHDVPDLYLAPVVLAADAKLDDLATLEPRALAQRVAIESN
                     APAWSRENREKGLLVAIAHFVDCRGWTLTWDPRGLRLSHDEHTVVLGVPASFADYLSG
                     GGVGV"
     gene            6023..6901
                     /locus_tag="VGD55_01420"
     CDS             6023..6901
                     /locus_tag="VGD55_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY0869029.1"
                     /translation="MSLEQEAREAELTERVVASFVDTPDPRLKVLIEALTRHLHAFAC
                     EVRLTEREWQQAIDFLTAVGNITDECRQEFILLSDVLGISMQTITINNQAHGDATEAT
                     VVGPFFLQGSPRIEIGGDIANGAPGEPCWVEGVVTDSTGSPVSEARVEVWEADADGRY
                     DVQYGDTRVSGRGHLFADDEGRYAFWGVKPTSYPIPHDGPVGAFLKAVQRSPVRAAHL
                     HFMVSAPGLRTVVTHIFVDGDDLLDRDSVFGVKDSLVRVFHAQPAGAATPDGRDLAGQ
                     PWSRVRFDIVLAPSAP"
     gene            6898..8316
                     /locus_tag="VGD55_01425"
     CDS             6898..8316
                     /locus_tag="VGD55_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869030.1"
                     /translation="MRPPASWRGVALLVAGAFFMENLDGTIIATAAPRMAASLGVRSV
                     DLNVVITAYLLTLAVFIPVSGWIADRVGARTVFAAAIALFTVASGFCAVSTSLGELTA
                     VRVLQGIGGAMMVPVGRLVVLRSTTRADLIDAIAYLTWPALAAPVIAPALGGVLTTYA
                     SWRWIFVINLPLGVAAFAIAMRIVPNVRVGGHAALDWRGFAFTGVGLAALVYGLELCT
                     ASRVAWAVVAVSLAVAALSLTIAVRHLRHARFPLMNLAAVGIPTFRVMAIGGSFFRLA
                     IGAVPFLLPLMFQDAFGWTSLKSGLLVVAVFVGNIGIKPATGPLLRRLGFRRVLVVAG
                     IGAGVTVLLCGFLTAHTPLVVIVLVLLSSGVFRSIAFTAYNTIAFADIAPDDVGSANT
                     LSATLQQLTMGLGVAVGALALRAGGPIARLFGASGAGASPFAVGFVIVAALAFVAVAD
                     SVLLRPDAGEHIISRPDVGVAS"
     gene            complement(8334..9296)
                     /locus_tag="VGD55_01430"
     CDS             complement(8334..9296)
                     /locus_tag="VGD55_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY0869031.1"
                     /translation="MSPEEPDLLQGPAELVRAATVARLYFLEGKSKSDIADELGINRF
                     KVARILESAQESGVVKIQISVPSGIDDALSEQVRRAFGLRHVVAVDAGDVHELHIRKA
                     LARTATALLSELVTEADVLGVGYGRTLTIMAESMTSLARCPVVQLTGALLGVNMEDNS
                     VELVRQISARNGGSAFPIYAPQVLPDAQTAATLRLQPEVAEALRRFPTVTKAVVAVGS
                     WNPAASQLYDALSPKERKPLVQRGAVAEVCATLLDADGNVVAQDFADRCISISGDELK
                     AIDEVIAVAGGTQKTAAVRSVLVGGYANSLVTDTSLARSLLATQ"
     gene            9422..>10152
                     /locus_tag="VGD55_01435"
     CDS             9422..>10152
                     /locus_tag="VGD55_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0869032.1"
                     /translation="MAVGYRRRGGLVSLVALLSVGSMVLAGCGSSKHTATGSTSSSPA
                     TGATTSAPAPSAASGAASTPAAASSTAVASSGGSSGATYTGPPVTITVAVTANPLMTT
                     IEKLTPAGFEASHPNIKVKFVTYDENTERADVEKDVAASGGQYDVVMIGPNDIASWAK
                     NGWIKDLTTDAAADPSYDVGDLLKPISDALSSGGKLYAVPFYGESSFMMYNKSMFAAA
                     GVTMPANPTWDQIAALADKLNNPSKG"
BASE COUNT         1764 a         3151 c         3331 g         1906 t
ORIGIN      
        1 gtcctcgaag cactcaagac gacgctcgac gagaaccttg cgatgatccg cgacaccgtg
       61 ttgcacctgc gcggtgaggg acgccgggtg ttcgttgacg ccgagcattt tttcgacggc
      121 tacaaggcga atcctgcgta cgcactcgag gcagtcaggg tggcggccga ggccggcgcc
      181 gacgtcatcg tgttgtgcga cacgaacggc gggatgctcc ccacgcagct cgccgacgtc
      241 gtccacaccg ttttgcaaag tagtggcgcg cgcatcggta tccactgtca cgacgacact
      301 ggctgcgcgg tggcgaacac gcttgctgcc gtcgaggccg gcgtcacgca tgtgcaaggc
      361 gtcgtcaacg gctacggcga gcgttgcggt aatgcgaatc tcgtgacggt cgtggcaaat
      421 ctcgaactga agctcggacg ccgagtgctt ccggatgacc gagtagtcga gctcggccgg
      481 gtctctcacc tggtggctga gatcgcgaac cagccgccgc gcgcccacca gccgtacgtc
      541 gggctgtccg cgttcgcgca caagggcggg ctccacgcat cagcgatcaa ggtgtcggcc
      601 gatctctacc agcacatcga tccttcgctg gtcggaaacg acatgcgcat gctcgtttcg
      661 gaaatggcgg gccgagccag cgtcgagatg aagggcaggg agctcggatt cgacctgtcg
      721 gatgacaagg acgtcgttgg ccgggtggta gaccgggtga agtcgcttga agcaaagggc
      781 tacatgttcg aggcggctga cgcatcgttc gagttgttgt tgcgtgaaga gatcaatggc
      841 gagccgcttc agtacttcac gctggagtca tggcgcgtga tcgtcgagca gcgcgccgat
      901 ggcgaggtgg tctctgaagc gacagtgaag gtgcaagcca agggcgagcg gatcgtgtcg
      961 accggcgagg gcaatggtcc ggtcaacgct ctcgaccgtg ctttgaggct cgcgctcgaa
     1021 cggttgtatc cgcagctcgc tgaactcgag ctggtcgact acaaggtgcg catcctcgaa
     1081 ggcgcgcacg gcaccggcgc gatcacccgg gtgctgatcg agacgagtga cggaaagact
     1141 gagtgggata ccatcggcgt cgacgagaac ctcatcgccg cgtcgtggca ggcgatggaa
     1201 gacgcctaca cctacggtct gctccgccag cactcgtcct gagccgataa cctcagcagc
     1261 gtgcgaatag cgagattctc ttacggcgac cctgatgtcg ctgggtatgc gttcggggcg
     1321 atcggtggca cgccaggcgc cgaagaggtt gcggtcatca acggccaccc cttcggcgag
     1381 atcacgttca gcgggctgcg gctgccgctg gcgggagtcc ggctgctgcc tcctgtgttg
     1441 cccagcaagg tggtggccat cggtaagaac tacgccgacc acgcacgcga gatgggcggc
     1501 gagccaccag ccgagccggt tctgttcctc aagccctcga cgtccgtcat tgggcctggc
     1561 gctgcgatca cctacccgaa gttgtcgcag aaggtcgact tcgagggcga gcttgccgtc
     1621 gtcatcggcc ggctttgtcg cgaggtgcca ctcgagcggg tgcccgaagt gattcttggg
     1681 tacacctgcg cgaacgacgt cacggcccgc gatttgcaga agtccgacgg gcagtgggcg
     1741 cgggcgaagg gcttcgacac gttctgccca ctcggtccgt ggatcgacac cgaggtcgac
     1801 ccgtctgacc tcgcgatcac cacgacggtg aacggtgacg tcaagcagaa cgcgcgcacg
     1861 tcgttgctat tgcacgacgt cgcagaactc gtcgtattcg tcagcgccgc gatgacattg
     1921 ctgccgggcg acgtcattct cacgggcaca ccggccggcg tcggaccaat cgtgcctggc
     1981 gacgaggttg cagtgaccat cgaagggatc ggcacgttga ccaacaccgt cattggcacc
     2041 ggggtctcgc gatgagcgtc aggttgcggg tagcaccgtc accgaccggc gacccacatg
     2101 tcggaacggc gtacatgtcg ctgttcaatc tcgcgttcgc gcgccagcag ggtggcgcgt
     2161 tcatcctgcg catcgaagac accgaccgcg gtcgttacgt cgaagacagc gagcagcaga
     2221 tttacgacac gctgcactgg ctcggtctcg attgggacga gggccccgac atcggcggcc
     2281 cgttcgctcc ctaccggcag tcggagcggc tcggcaccta tcggccgttc ttcgagcggc
     2341 tgctcgccga cgggcatgcc tactactgct ggtgttctgc cgagcggctg acgcaaatgc
     2401 gcgaagacca gcaacgccgc aaacaagcga cgggttacga ccggctctgc tacggcaaga
     2461 cgcgcgagga gcgggcattg ctgccgggct tcagcgagac gcctgttgtg cgcatgctga
     2521 tcccgagtga cgtcgagctc acgttcgtcg atctcattcg aggccggacg tcggccccgc
     2581 acccagacga tcaagtgatc gtgaagggcg acggtttccc gacgtaccac ctcgcggttg
     2641 tcgtcgacga ccacttgatg gggatcactc acgtcgtgcg cggcgaggaa tggatcagct
     2701 cgacgcccaa gcacatcttg ttgtacaaat ggttcggcct cgagccgccg gcgttcgcgc
     2761 atatgccgtt gctgcgcaac atcgacaagt cgaagatcag caagcgcaag aatccggctg
     2821 cccggttgct gtggtttcgc gagcagggat atctccccga ggcattgcgg aatttccttg
     2881 cgttgatggg ctactcgatg ccggacggtc gcgagatctt cagcttcgac gagatggtgg
     2941 agagcttcga ctggacccga gtgaacccgg tcggccccgt gttcgacatc gacaaactca
     3001 actggttgaa cggccagtac cttcgagcgc tgtcggtcga cgacctagcg actaggttgc
     3061 tgccgattct tcagcgcgcc gatgtgatcg cagacccacc agccgcaggc gacgtcgaac
     3121 ttttgcgtgc ggcggctccg cttgtgcatg aacgcatggc gctgctgtct gaggtcatcg
     3181 acatgctcgg ctttctgttt gtcgacgacg atgatttcga gcccgatgct gatgccgcag
     3241 cgaagaactt gggtaccgac gccgcacccg ctttggacgc cgccttgagc gcgctcgcgt
     3301 ccgttgggca ctggcatgcc gacgagatca aggcggccct cgaggtagca ctcatcggtg
     3361 aactcggcct gaagccgcgc aacgcctacg cgccgttgcg ggtcgcgatc accggacgca
     3421 cgatctcgcc gccgctgttc gagtcgatgg aattgctcgg ccgagaacgg agcatgtcga
     3481 ggcttcagcg ggccgcggcg tccaacgcct aggtccttct gggtactttg ggtgccttgg
     3541 tcgcggtcct atacactcga ccagcgatcg gaagaggcct gtccgatcag atggggtatg
     3601 gggtaattgg cagcccggct gattctggtt cagctagtct aggttcgagt cctggtaccc
     3661 cagccaggtc gggaattctc cggccggtga tgccttcaca aatcgcacgg ccccgttgtc
     3721 tagcggccta ggacgccgcc ctctcaaggc ggtagcgcgg gttcgaatcc cgtcggggct
     3781 acttctcggc cgcagcacgt tcgtggagga gagccatgtc ggcgaacacc ccgcgcattg
     3841 ctgtcgtgac cggcggcggc agcggcatcg gagcggcggt cgcactcgcg ctggcagccg
     3901 acgcctggac cgtcgtcgtt gcggggcggc gcaaggaggc actcgacgag accgtcgccc
     3961 gcggcttcgc ctccaacggc acactcgagg ccattccgac cgacgtcacc gacgaggcga
     4021 gcgtgcgcgg actcttcgac gccgtcgtcg atgctcacgg ccgggtcgac ctgctgttca
     4081 acaatgccgg aaccggagct cctgccgtcg acattgatgc tctcgagctc gacgcgtgga
     4141 accaggtggt cgcggtcaat ctgaccggcg cattcctgtg tacccgcgaa gcgtttcggg
     4201 tgatgcgaaa ccaggagccg cgcggcgggc gcatcatcaa caacggctcg atctccgcac
     4261 attcgccacg gccacactcg atcgcgtaca ccgcgacgaa gcatgcgatc accggcctga
     4321 ccaagtcgac gtccctcgat gggcgagcgt tcgacattgc atgcgggcag atcgacatcg
     4381 gcaacgcagc taccgacatg gcgggcggtg ctgcccgcgg cgccgcacaa gccgacggct
     4441 cggtgcggcc agagccgttg atggacgttg gcgaagtcgg gcgcgcggtg tgttacatgg
     4501 cgagcctgcc ccttgatgcg aacgtggctt cactgatagt gctgccacca aagatgccgt
     4561 tcgtcgggag ggggtaaggc gatgccgaac tcagagctcg gtgccaccgc atgggaccac
     4621 gatcggtcga tcgtccatgg cccggatccg gcgatcgagg tgattgacga gcgcttccgc
     4681 tctcttgtcg tggggtctga gatcgtcgag cgcttgtgga ccggcggtcg ctggctcgag
     4741 gggccggtgt ggttcgggga cgcacgccac ctgctgttct ccgacatccc caacaaccgg
     4801 atgctctgct ggagcgaggt caccggcgcc gtgaccgtgt tccgcgagcc gtcgaacaac
     4861 agcaacggca acacccgcga tcggcagggc cggcttgtga cctgcgagca cctgacccgt
     4921 cgggtgaccc gaaccgagta cgacggcaac atcaccgtct tgatggacag ctaccaagga
     4981 aagccgctga acgccccgaa cgatgtgacc gtgcattccg acggatcggt gtggttcacc
     5041 gatcccggtt ggggcatcga aggcaactac gaaggcgtga aagcagtcaa ggaaattccg
     5101 cgctacgtct acagaatcgg cgcggacggc gagggcgtcc cggtcatcac ggacatggac
     5161 cgacctaacg gcatctgctt ctcaccggac gagtcattgc tgtacgtcgt cgacgtcgac
     5221 cagatccggg tgtacgacat ggacggcacg aagccggtca acggccgagt gttcgtggca
     5281 atgaagggct ccgacggcat ccgtaccgat ctcgacggca acgtgtggtc ggcggccggt
     5341 tggggtgggc caggcttcga tagcgtgcac tgctacagcc ccgcgggcga actgcttggg
     5401 cagatcaatt tgcccgagac gtgcgctaac ttctgcttcg gcggagccaa gaggaaccgg
     5461 ctattcatca ccgcgagccg atcgctgtac agcgttttcg tcgaggcgca aggatgccag
     5521 cgaccctgac gcatcagggc tcagacgccg acgccgcccc ctgacaggta gtcggcgaag
     5581 gatgccggca caccgagcac gaccgtgtgt tcatcgtgac tgaggcgcaa tcctcgggga
     5641 tcccacgtca gcgtccagcc gcggcagtcg acgaagtgcg caatcgcgac cagcaggccc
     5701 ttctcccgat tctcgcgact ccacgcgggt gcgttgcttt cgatcgcaac tcgctgcgct
     5761 agcgcgcgtg gctccaaggt ggcgagatcg tcgagcttcg catctgcagc gagcacaacc
     5821 ggtgcaaggt acagatccgg gacgtcgtgg ggcaggttgc gcacggaaag cctcctcgtc
     5881 gagagctagc tcgttctaga tccatgctcg ggcgacgcgg tcccggcggg cagggtagga
     5941 ggtccttgat cgcgtggacg ttcgtcgtgc cgccggagac tcgatagcct tgctgcactg
     6001 cactcggaag gagccgaccg aggtgagcct cgagcaagaa gcccgagaag ccgaactgac
     6061 cgagcgagtg gtcgcctcgt tcgtcgacac tccagacccg cggctcaagg tgttgatcga
     6121 ggcattgacg cgccatctgc acgcgttcgc gtgcgaggtt cgattgacag agcgcgagtg
     6181 gcagcaggcg atcgattttc ttactgccgt cggcaacatc accgacgagt gtcgtcaaga
     6241 gttcattctt ctttccgacg ttctcggcat ctcgatgcag acgatcacga tcaacaatca
     6301 ggcgcacggc gacgccaccg aggcgaccgt cgtcggaccc ttcttcctcc aaggctcgcc
     6361 ccgcatcgag atagggggag atatcgcgaa cggcgcaccc ggggaaccat gctgggtcga
     6421 aggagttgtc actgactcga cgggtagccc ggtgtctgaa gctcgcgtcg aggtctggga
     6481 agccgacgca gacggtcgct acgacgtgca atacggcgac acgcgggtct ctgggcgcgg
     6541 gcaccttttt gccgacgacg aaggccgcta tgcattctgg ggcgtgaaac cgacctctta
     6601 ccccattccg cacgacgggc ccgtcggagc gttcttgaaa gcagttcaac gttcgccggt
     6661 gcgcgcggcg catctgcact tcatggtgag cgccccgggc ctgcgcaccg tggtcacgca
     6721 catctttgtc gatggcgacg acctgcttga ccgcgacagc gtgttcggtg tcaaggactc
     6781 gctggtgagg gtgtttcacg cacagccggc cggcgcggcg actcccgacg gccgtgacct
     6841 cgccgggcaa ccatggtcgc gcgtacgttt cgacatcgtg ctggcaccat cagctccgtg
     6901 aggccgccgg cgtcgtggcg tggcgtagcc cttctggttg ccggtgcatt cttcatggag
     6961 aacctcgacg gcacgatcat cgcgacggcc gcacctcgca tggcagcttc gcttggcgtg
     7021 cggtccgtcg atctcaacgt cgtcatcacc gcctatctgc tgacgttggc ggtgttcatt
     7081 ccggtgagcg gctggatcgc ggatcgagtg ggtgcccgca ctgttttcgc ggccgctata
     7141 gcgctcttca ccgtcgcctc tgggttttgc gccgtgagca ccagccttgg cgaactgact
     7201 gcggtgcgcg tcttgcaggg gatcggcggg gccatgatgg tgcccgtcgg caggcttgtt
     7261 gttcttcgca gcaccacgcg cgcggacttg attgacgcga tcgcgtacct gacctggcca
     7321 gctttggctg cacctgtcat cgcgcctgca ctcggcggtg tgctgacgac ctacgcatcg
     7381 tggcgctgga tctttgtcat caacctgcca ctgggagtag ccgccttcgc gattgcaatg
     7441 cgcatcgttc cgaatgtgcg cgtaggtggg cacgcggcgc tggattggcg cggatttgcc
     7501 ttcaccggcg ttggccttgc tgcgctcgtg tacggcctcg agctgtgtac cgcgagccgc
     7561 gttgcctggg ccgtcgtcgc ggtgtcgctc gccgtcgcgg ccctctcgtt gacgatcgcc
     7621 gtacgccact tgcgacacgc gcgcttcccg ctcatgaacc tggctgcggt gggaattccg
     7681 accttcaggg tgatggcgat cggcggttcg ttcttccggc tcgccatcgg agccgtcccg
     7741 ttcctgctgc cgctaatgtt ccaggacgcc ttcggttgga cctcgttgaa gtcgggcctg
     7801 ctggtggtcg ccgtcttcgt cgggaacatc ggtatcaagc cggcgacagg tcccctactg
     7861 aggcggctcg gttttcgacg agtgctggtg gtcgcgggca tcggcgccgg cgtcacggtc
     7921 ttgctgtgcg ggttcttgac tgctcacacg ccattggttg tcattgtgct cgtgctgttg
     7981 tcgagtgggg tattccggtc gatcgcgttc accgcctaca acacgatcgc gttcgccgat
     8041 atagcacccg acgacgtagg gagcgccaac acgctttcgg ccacattgca acaactcacg
     8101 atgggcctcg gagtcgcggt tggtgctctc gcgcttcgcg cgggcggccc gattgcacgg
     8161 ctcttcggtg cgtctggcgc tggcgcaagc ccgttcgccg tggggttcgt gatcgtggct
     8221 gcgctggcct tcgtcgcggt tgcagactcg gtattacttc ggccagatgc aggggagcac
     8281 atcatctcga ggccggatgt cggcgtcgcg agctgaaccg tcggcaggtg aaattattgg
     8341 gtggcaagca agctacgtgc gagagacgta tcggtgacga ggctgttggc gtaaccgcca
     8401 acgaggacag atcgaaccgc agcggtcttt tgcgttccgc cagcaactgc tatgacctcg
     8461 tcgatcgcct tcaactcgtc gccgctgatc gagatgcagc ggtccgcgaa gtcttgagcg
     8521 acgacgttgc catctgcgtc gagcagggtt gcacacacct cggcgacggc gccgcgctgg
     8581 accagcggct ttcgttcctt cggtgacaac gcgtcgtaga gctgggacgc cgccggattc
     8641 cacgacccga cagcgacgac agccttggtc acggtcggaa agcgacgaag agcctctgcc
     8701 acttcgggct gcaagcgcag tgtcgcagcg gtctgcgcgt cgggtagcac ctgcggtgcg
     8761 tagatcggaa aggctgaccc gccgttgcgc gccgagatct ggcggacgag ttcgacgctg
     8821 ttgtcctcca tgttgacgcc gaggagtgcg ccagtgagtt ggacgacggg acaccgcgcg
     8881 agcgacgtca tgctctccgc catgatcgtg agcgtgcggc catagccgac gcccagaacg
     8941 tcagcctccg tcaccagctc tgacaacaac gccgtcgcgg tgcgcgccaa cgccttcctg
     9001 atgtgtagtt cgtggacatc tcccgcatct acagcaacga catggcgcaa gccgaatgct
     9061 cggcgcacct gctcggacaa cgcatcgtcg atgcccgagg ggacggaaat ttggatcttt
     9121 acaacgccgg actcctgcgc gctttcgagt attcgggcga ccttgaagcg attgatgccc
     9181 agttcgtctg cgatgtcgct ctttgacttt ccctcgagga agtacagccg cgccacggtg
     9241 gcggctcgga ccagctcggc tggtccctgc aagaggtcgg gctcctccgg cgacatgacg
     9301 gctccacgta acgaatcggc aacagggctt gacggcacgg tccaaatggg ctcgactgag
     9361 tgctcgtacg agcatgctat ctgctcaaat gagcaaacgc aactatcgtc aaggaggagc
     9421 agtggctgtc ggttatcgcc ggcgaggcgg cctggtgtcg ctcgtcgctt tactgtctgt
     9481 tggttcgatg gttctcgccg gatgtgggag cagcaagcac acggccactg gttcgacgtc
     9541 ttcgagtcca gcaactgggg cgacaacttc ggcgcctgca cccagtgctg catccggtgc
     9601 tgcatcgaca cctgcggccg cgtcgtcgac ggcagtggcg tcgtcgggtg ggtcgtcggg
     9661 cgcaacctat acaggtccgc cggtgacgat caccgtggcc gtgacggcga accccctgat
     9721 gacgacgatc gagaagctga cgccagccgg gtttgaggcg agccacccca acatcaaggt
     9781 gaaattcgtt acctatgacg agaacaccga gcgcgccgac gtcgagaagg acgtcgcggc
     9841 cagtggcggc cagtacgacg tcgtcatgat tggtcccaac gacatcgcgt cgtgggcgaa
     9901 gaacggctgg atcaaggatc tgaccactga cgcggcggcc gacccgagtt acgacgtcgg
     9961 cgacctgctg aagcccatca gtgacgcgtt gtcgtcgggc ggcaagctgt acgccgtgcc
    10021 gttctacggc gagtcgtctt tcatgatgta caacaagtcg atgttcgcgg cggccggcgt
    10081 gacaatgccg gccaacccga cgtgggacca gatcgcagcg ctggccgaca agctgaacaa
    10141 cccgtcgaag gg
//
LOCUS       DASWVF010000082         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_27679, whole genome shotgun sequence.
ACCESSION   DASWVF010000082 DASWVF010000000
VERSION     DASWVF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27679"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGD55_01440"
     CDS             complement(<1..243)
                     /locus_tag="VGD55_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0869033.1"
                     /translation="MTTPVDTRGRGVRDLRVSVTDRCNLRCRYCMPREVFGPGFVFLP
                     REELLRFEEITRIVAAFARAGVTKVRLTGGEPLLRAG"
     gene            complement(240..1736)
                     /locus_tag="VGD55_01445"
     CDS             complement(240..1736)
                     /locus_tag="VGD55_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY0869034.1"
                     /translation="MNPSRPRPSGAGYTVTMRVMAPHDPTLLPRLAEVVSEARGVVTG
                     MDLVDVTTSGATVDLTMLALDEDHVHEVAAALEAETGVKVRHTSDPTFLYHLGGKIQV
                     TARTSLRTRQDMSMAYTPGVARIASAIAVRPESAWTLTAKGSSVAIVTDGTAVLGLGD
                     IGPLGALPVMEGKSLIFKEFGGVNAYPLCLNAKGPQAVIDATLAIAPGFGGINLEDIA
                     APNCFQVETELQRQLDIPVFHDDQHGTAVVVSAALLNACRITGRTFPDVRIVHVGCGA
                     AGTAICSALLDAGVKDLIIFDKDGVLTEGPTTPAHHRTLAARSNPRGITTMEEALRDA
                     DVFIGTARRGSVDPDLLQAMAPQPIVFALSNPEPELLPDEARKDAILATGRSDMPNQI
                     NNALCFPGFFRGALDARATTVTLAMKQAATRAIADIVTDDQRGLGVIIPTMFQARLHQ
                     AVARAVSDAWLVEHPDGRKREPNLLGQPGIDFGLPDLPAPIPSDGGSL"
     gene            1791..2393
                     /locus_tag="VGD55_01450"
     CDS             1791..2393
                     /locus_tag="VGD55_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY0869035.1"
                     /translation="MSLAVAAIAFGVVFLAELPDKSMVASLVLGTRYRPLYVWVGAAA
                     AFAVHVVIAVAAGGALSLLPHRLVEGIVAVLFFAGAALLLFGKESREEERGVDETGEA
                     VEIAAPTLRRVASVSFAVIFVGEWGDITQIATANLAAKYHDPIGVGVGALLGLWTVVA
                     IGVVAGSKLVERVPLALVRRITGVILAVLGVVSVVAALRG"
     gene            2428..>3197
                     /locus_tag="VGD55_01455"
     CDS             2428..>3197
                     /locus_tag="VGD55_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0869036.1"
                     /translation="MTPTQLRAYSAVVRHGSVKDAAAEIGVTEAAVSLHLGQLRKELS
                     DQLFTRTAVGLAFTPGGLRLASRAAEMLGLQAQTIREVTQAGHGRRLLRVAATSLFAE
                     YAAPGLIEMFVGRADDFDVELSVRPPSQFEALLASRAVDVAIGPRMPNRPGALVCKPF
                     LNYHVVAVVGSGHPLATVAVARPAVLKEQTWLLGPSAAGESGVVPQLLRQLDIPEERQ
                     RIYQSHAAALDEAKRNNGVALAASFAVSADLANGRLVVL"
BASE COUNT          501 a          995 c         1128 g          573 t
ORIGIN      
        1 tccggcccgc aacagaggtt cgccgccggt cagccgcacc ttggtaacgc cggcgcgggc
       61 gaaggcagcg acgatgcggg tgatctcttc gaagcgcagc aactcctcgc ggggcaggaa
      121 aacgaagccg ggcccgaaaa cctcacgcgg catgcagtaa cggcaacgca ggttgcagcg
      181 gtctgtcact gacactcgga ggtcgcggac gccgcggccg cgcgtgtcga ctggagtagt
      241 cagagcgagc cgccgtcgct cgggatcggc gccggcaggt cgggcaggcc gaagtcgatg
      301 ccgggttggc cgagcaagtt cggttcgcgc ttgcgcccgt cggggtgttc gacgagccac
      361 gcatcgctga ccgcgcgcgc gacggcctga tgcaaccgag cctggaacat ggtcgggatg
      421 atgacgccga gcccacgctg gtcatcggtc acgatgtcgg cgatcgcgcg cgtcgcggct
      481 tgcttcatcg ccagcgtgac ggtcgtcgca cgggcatcga gcgcacctcg gaagaagcca
      541 ggaaaacaaa gcgcgttgtt gatctgattg ggcatgtcgg accgcccggt cgcgaggatc
      601 gcgtccttgc gggcctcgtc cgggagcagt tccggctcgg ggttcgacaa ggcgaacacg
      661 atcggctgcg gagccatcgc ctggagcaag tcggggtcaa cggagccgcg tcgagcggtg
      721 ccgatgaaca cgtcggcgtc gcgcaaggcc tcttccatgg tggtgatccc gcgcgggttc
      781 gaccgcgcgg caagagtgcg gtggtgtgcg ggcgtggtcg gaccttcagt gagcacgccg
      841 tccttatcga agatgatcag atctttcacg ccggcgtcga gtaatgcgga gcaaatcgcc
      901 gtccccgctg caccgcatcc gacatgcacg atgcggacgt cgggaaacgt gcgcccggtg
      961 atgcggcagg cgttcagcag agccgcggat acgaccaccg ccgtgccgtg ctggtcgtcg
     1021 tggaagacgg gaatgtcgag ttgccgctgg agttcggtct cgacctggaa gcagttcggc
     1081 gcggcaatgt cttcgaggtt gatgccgccg aaacctggtg cgatggcaag cgtcgcatcg
     1141 atcactgctt gcgggccctt ggcgttaagg cacagcgggt aggcgttgac cccgccgaat
     1201 tccttgaaaa taagggattt cccttccatc accggcaacg cgccgagcgg gccgatgtcg
     1261 ccgagaccga gcacggcggt gccgtcggtg acgatcgcga cggacgatcc ctttgcggtg
     1321 agtgtccacg cagattcagg ccggacggcg attgctgaag caatgcgcgc gacgccgggc
     1381 gtgtaggcca tcgacatgtc ttggcgggtg cgcagcgagg tgcgcgcggt gacctggatc
     1441 ttgccgccga ggtgatacag gaacgtgggg tcggaggtgt ggcgcacctt cacgccggtc
     1501 tcggcctcga gtgctgcggc gacctcgtgc acgtggtctt cgtcgagggc gagcatcgtg
     1561 aggtcgacgg tcgcgccaga ggttgtgacg tcgacgaggt ccatgcccgt gacgacgccg
     1621 cgcgcttcgc tcacgacctc ggcgaggcgg gggagcagcg tcgggtcgtg cggggccatg
     1681 acgcgcatcg tgacggtgta cccagcaccc gagggccggg gccgactggg gttcatcggt
     1741 gaatcggtca tacctcatga tgccgtaaac cccgtcacta ggctcggcac atgagcctcg
     1801 ccgtggcagc gatcgcgttc ggcgtggtct tcctcgccga gctgcccgac aaaagcatgg
     1861 tcgcctcgct ggtgctcgga acccggtacc ggccccttta cgtctgggtc ggagccgcgg
     1921 ccgccttcgc cgtccacgtt gttatcgccg tcgcggccgg tggtgcgttg tcgctgctgc
     1981 cgcaccggtt ggtggagggc atcgtcgccg tcctgttctt cgctggcgcc gcattgctcc
     2041 tgttcggcaa ggagtcacgt gaagaagaaa ggggcgtcga cgagacgggt gaggccgtcg
     2101 agatcgctgc accgaccctg cgccgggtcg cgtccgtgag cttcgcggtg atcttcgtgg
     2161 gggagtgggg agacatcacc cagatcgcga ccgccaactt ggcggcgaag tatcacgatc
     2221 cgatcggcgt cggtgtcggc gccctgctcg gattgtggac cgtggttgcg atcggcgtcg
     2281 tggccggctc gaaactcgtc gagcgggtgc cgttggcctt ggtgcgtcgc atcaccggag
     2341 tgattcttgc cgtgctcggt gtggtcagcg tggtagccgc actccgaggt tgaccacgca
     2401 cctcgctgtt tcgattagat tgcgatgatg accccgaccc agttgcgtgc ctactccgca
     2461 gtggttcgac acggctcggt gaaggatgcc gccgcggaga tcggcgttac cgaagcggcg
     2521 gtatcactgc atcttgggca actgcgtaag gaactctccg accaattgtt cacccgcacg
     2581 gcagtggggc tggctttcac gccgggtggc ctgcggttgg cgagcagggc agcggagatg
     2641 cttggcctcc aggcccagac gatccgagag gtaacccaag ccgggcacgg ccgtcgactg
     2701 ctgcgcgtcg ccgccaccag cctcttcgcg gagtacgccg cgcctggcct gatcgagatg
     2761 ttcgttggcc gcgctgacga tttcgacgtc gagttgagtg ttcggccacc atcacaattc
     2821 gaggcgctgc tggcgtcacg tgcggtcgac gtcgccatcg gtccacggat gccaaatcgc
     2881 ccaggcgctc tcgtctgcaa gccgttcttg aactaccacg tcgttgctgt cgtcgggtcg
     2941 gggcatccgc tcgcgacggt cgctgtcgcc cggccggcgg ttctgaagga gcagacctgg
     3001 ctgctcggcc cgtcagcagc gggcgagagc ggcgtcgttc cccaactctt gcgccaactc
     3061 gatatccccg aggagcggca gcggatctat caaagccatg ctgccgcgct tgacgaggcg
     3121 aagcgcaaca acggtgtcgc gctcgcggcc tcgttcgcag tctccgccga cctcgccaac
     3181 ggccggttgg tcgtgct
//
LOCUS       DASWVF010000083         7731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_180569, whole genome shotgun sequence.
ACCESSION   DASWVF010000083 DASWVF010000000
VERSION     DASWVF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180569"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VGD55_01460"
     CDS             complement(<1..1829)
                     /locus_tag="VGD55_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0869037.1"
                     /translation="MTSPAEEPPSRGWALWWGYLTLIFVGSGVLIAASIRVLSHQHLH
                     GSATAFGLLAVLLVLAEMRPVVASGSYDADGVTVSTAFVFAIVLIWGPAPAIVVQFAA
                     ISIREILMRKTWWRVMFNAGQYAICTGVAAVVLRVFGSRPSLSHPDLVRGGILIGLAV
                     AAVTYHMLNLFLVGVILSIRKGSTIWAEIVDEYWYYTSTTMAVIALSPVLAVVAVHSW
                     QMIPLLLLPLFLVYKTASISLEREQQALHDALTGLANRKLLLAHTSEVLDDLQLADGR
                     VALVLLDLDRFKDVNDTLGHPVGDRVLQVVATRLQDAVRPGDLVARLGGDEFAVLLRG
                     LDSAATVTEVAERIRTSLHEPISFDGTMVDLDASLGIALYPDHGEDFDQLHRRADVAM
                     YVAKSSGGGVAIYDVEKDTNSLAKLGLLGELRHAIDSGQLELCYQPKIELSTGTVVGV
                     EALVRWMHPTLGMVMPDDFIPLAEQSGLMPHLTNLLLDQALAQRAGLDANAPGLVVAV
                     NISVRDLLDPGFADRVAAALARHRIPPDALMLEITERRLLADYDRGAKVLAELAASGV
                     HISLDDFGTGYSSLTLLKRLPVCELKIDQSFVSRIGESEADAT"
     gene            2008..4146
                     /locus_tag="VGD55_01465"
     CDS             2008..4146
                     /locus_tag="VGD55_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0869038.1"
                     /translation="MKQQGMKQQGAEQPRVEPADLQEVAQRVLAALAGPSALLRPDQL
                     VAARALVCDRRRVLCVQATGWGKSAVYWIAAAALRAAGSGPVLVVSPLLALMRDQVTA
                     AERAGLRAVTLNSSNFDDWAAIEKQLADDEVDVLLTSPERLASARFAETVLPNLLSRL
                     GMLVIDEAHCVSDWGFDFRPDYQRLTRLLTSTRDDLPVLATTATANDRVTRDVATQLG
                     AGSAVEPLVLRGTLARSSLQLGVVPGLTALQRYAWVAAALDRLEGSGIVYVLTVADTY
                     RLAEFLTSRGHAVAAYSGQLETDEREQVEDRLRRNEIKAVVSTSALGMGYDKPDLAFC
                     LHVGSPASPVAYYQQVGRAGRALDTAVAVLLPAETDERVWAYFADASTPDPAVAQRVL
                     ATLNESGEPLSLPKLEDSTGARRGRLESLLKILAVDGAIRRVGGGWEATGEPWVFDDE
                     RYAALGAARAAEAGIMRSYAAGAGCLMSFLRLSLDDPHAGEPCGVCSVCTGALPAGLA
                     SKPTTADIDAARVFMRGVDVVIEPRKLWPSGGARKGRIGTVLAEGRALAFADDPGWAD
                     LVVRLTREGAPDAELPSEVAAGVVELLARWARQWPERPTAIAAIPSRAHPLLVASLAR
                     HIAEVGHLELLAPFEIDGPVPVSEVASAQRVAAVASSLRLAAGVEMPPGPILLVDDVY
                     TSGWTMTVAAAMLGEAGAAAVYPLVLHRRP"
     gene            4185..4406
                     /locus_tag="VGD55_01470"
     CDS             4185..4406
                     /locus_tag="VGD55_01470"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEY0869039.1"
                     /translation="MADDDILARISDLVAEEHHLTGTEGAADRRRQIEEQLDQCWDLL
                     RQRQAKREFGDNPDEAHARPIPEVEHYQQ"
     gene            complement(4458..>7731)
                     /locus_tag="VGD55_01475"
     CDS             complement(4458..>7731)
                     /locus_tag="VGD55_01475"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HEY0869040.1"
                     /translation="LAAAGLQATTAGAVPPPVTQAPGAPGALSHFDQSRKDCLGTARN
                     TTSKIWYTVANGVLSDVYAPTVDTTNVETMQFLVSDGSAFTDLQSRDTTYTASADRSG
                     MACTIVSTSNSHGYQLRTTYLTDPARDSVIMHTQFLPFTAAARHYQLYVRLDATAGGN
                     GGGGTGNGGADSATTDTSTGQPVPVSFDTVTATNAANRDYAVPTYLALHADRPFTAVS
                     SGFVGTPSDGLAELDATHTLSPTYTDATSGNVEQTARVNLGLFNSTTIALGFGTTQSA
                     AVHTAAMSSSQNYLGALAVYESQWQRYDDGLRAPATHLAGLTNAQARQTAAAYYVSAN
                     VVKASEDKTFPGAIAASLTSPWGQAVSAGDPANTYFGSYREVFSRDLYEAWTALFTDG
                     DINTARDAVRFLFLRQQLPDGSMPRNSLLNGKVAPDSFNTQLDEAAYPILMAQQSGLG
                     NDATLWPHIKAAANFLVSHGPAFGVERWEEQSGYSPSTIAAEIAGLVAAGTIAQQHGD
                     SADAQVYLATADLYQRSIKSWDVTTTGPLSSSPYFIRLSKTGDPNAAITYNVGNGGPD
                     LDQRSVIDAGFLELVRLGELSASDPVVANSLSVVDATIERTTPSGPGWLRYNGDGYGD
                     CYAPEPLTSCGTTGTPWAPSGQGTGHIWPVLSGERGEQDLATGQSATAADLLMSIGQL
                     AGGVGLVPEQDWDFANVAASPFGTDPTVASIGYTDGQPDGSAAPLTWGAAAQVRLTAD
                     LAAGRVVEKPSSTIARYVTHTQAATTLTVTSPGNNTAATGTVPVIGTTAPSAKVVIAD
                     VATDNNDATTLVSTTAALDGSFTMPVTVAAGTNVLVVTAMTASGATAQTTVTLINDVV
                     NGTLLFTATDPDNDDNGPGNYAYPTSSNFVPGAFDLEQFQVYDTGTTVTFRVQTRDLT
                     STFGSTNGAQLVDLYVHNPAATTTSTSAAFASRHYSIAAADAWSRLIEVQGFGQAFVD
                     ASGTTQGTVTISANSISRWTTFSVDKTALGGTPSSGWAFTIALTGQDGFGQDNARTFA
                     ATPQPFNFGVCATALATPLCTADPNTVPKVMDTVTPAGVLQPTELDYTLGPVVLQGVV
                     MP"
BASE COUNT         1231 a         2530 c         2672 g         1298 t
ORIGIN      
        1 atcgtcgcgt cggcctcgga ctccccgatc cgcgagacga acgactggtc gatcttgagc
       61 tcacacacgg gcagccgctt gagcaaggtc agcgacgagt agccggtgcc gaagtcgtcg
      121 aggctgatat ggactccgct cgccgccagc tcagccagca ccttcgcgcc gcggtcgtag
      181 tcggcgagca gccgccgctc ggtgatctcg agcatgagcg cgtccggggg gatccggtgc
      241 cgcgcgagcg ctgcggcgac gcggtcggcg aagcccgggt cgagaaggtc gcgcacgctg
      301 atgttgaccg cgaccacgag tcctggtgcg ttggcgtcga ggccggcgcg ctgagccagg
      361 gcctgatcga gcaacaggtt cgtgaggtgg ggcatgaggc cggactgctc ggccagcggg
      421 atgaagtcgt cgggcatgac catgccaaga gtcgggtgca tccagcgcac cagcgcctcc
      481 acgccgacga ccgtgccggt cgagagttcg atcttgggct ggtagcacaa ctcgagctgt
      541 ccggagtcga ttgcgtgccg cagttcgccg agcaggccga gcttcgccag ggagttcgtg
      601 tccttctcga cgtcgtagat cgccaccccg ccgcctgacg acttcgcgac gtacatcgcc
      661 acgtcggcgc gccggtgcaa ctgatcgaaa tcctcgccgt ggtcgggata caacgcgatg
      721 ccgagacttg cgtcgagatc gaccatcgtg ccgtcgaacg agatcggttc gtgcagcgac
      781 gtgcggatcc gctcggcgac ttcggtgaca gtggctgcgc tgtcgagacc gcggagcaac
      841 accgcgaact cgtcaccgcc gagcctggcg acaagatctc ccggccgaac cgcgtcttgc
      901 aaacgggtgg cgacgacctg cagaacgcgg tcgccgaccg ggtgcccaag cgtgtcgttg
      961 acgtctttga acctgtcgag gtcgagcagc acgagggcga cacgaccgtc ggcgagctga
     1021 agatcgtcga gcacctcact ggtgtgggcc agcagcaact tgcggttcgc caggccggtg
     1081 agggcgtcgt gcaacgcctg ctgctcccgc tcgagtgaga tcgacgcggt cttgtagacg
     1141 aggaacagtg gcaacagcag caacgggatc atctgccacg agtggacggc aaccaccgcg
     1201 agcaccggcg acagtgcgat cacggccatc gtcgtcgagg tgtagtacca gtactcgtcg
     1261 acgatctcgg cccagatcgt cgaccccttg cgaatcgaga ggatcacgcc gacaaggaac
     1321 aggttcagca tgtggtaggt cacggccgcg acggccaacc cgatgagaat gccgcctctc
     1381 acgagatcgg ggtggctcag cgacggtcgg ctgccgaaca cgcgcaacac cacagcggcg
     1441 acgccggtgc agatcgcgta ctgcccagcg ttgaacatca cccgccacca ggtcttgcgc
     1501 atcaagatct cgcggatcga gatggcggcg aactgcacga cgatcgctgg cgccggaccc
     1561 cagatcagca cgatcgcgaa cacgaaggcg gtcgagacgg tgacgccgtc agcgtcgtaa
     1621 gaacccgacg cgacgacggg tcgcatctcg gcgagcacca gcagcaccgc aagcaggccg
     1681 aacgcggttg ccgaaccgtg taggtgctgg tgcgagagca cgcgtatgga cgcggcgatc
     1741 aggacgcctg accccacgaa aatgagcgtc aggtatcccc accacaaagc ccaccctcgg
     1801 ctaggcggtt cctccgccgg ggacgtcacg ggcagccctc ttcccgtcgc ctgctcctgc
     1861 tcggtccgga gcgcactggc gaccatcgaa catctatcgg tcaggcggcc tctgactgaa
     1921 gggccgtttg gcacgatccg cctagtcggt cacctgcctg tggacgggac ggcggggggt
     1981 gtcggtccgg tgcgccatgc tcagggcatg aagcaacagg gcatgaagca acagggggcg
     2041 gagcaaccca gggtcgagcc cgccgacttg caagaggtcg cgcagcgggt gcttgccgcc
     2101 ctggcgggcc cgagtgccct actgcggccc gaccagctgg ttgccgcgcg ggccctggtg
     2161 tgcgaccggc ggcgggtgct gtgcgtgcag gcgaccggct ggggcaagtc ggcggtctac
     2221 tggatcgcgg ccgcggcgct gcgtgcggcc ggttcgggtc cggtgcttgt ggtctcgcca
     2281 ctgctcgccc tgatgcgcga ccaggtcacg gctgccgagc gcgctggcct tcgagcggtc
     2341 accctcaact cgtcgaactt cgacgactgg gccgcgatcg agaagcaact cgccgacgac
     2401 gaggtcgacg tcctcctcac ctcccccgag cgcctggcat cggcccgctt cgccgagacc
     2461 gtgttgccga acctgctttc ccgactcggc atgctcgtga tcgacgaagc acattgcgtc
     2521 tccgactggg ggttcgactt ccgccccgac tatcagcgtt tgacccgact gctgacgtcc
     2581 acccgtgacg atctcccggt tctcgcgacg accgcgaccg cgaacgaccg ggtcacccgc
     2641 gacgtggcca cgcagcttgg ggccggcagc gcggtcgagc cgttagtgct caggggcacg
     2701 ctggctcgca gctcgctgca gctgggcgtc gtcccgggcc ttacggcttt gcagcgctac
     2761 gcctgggttg ctgcggcgct cgaccggctc gaaggctccg gtatcgtcta tgtgctcacg
     2821 gtcgccgaca cgtaccggct cgccgagttt ctcacctcgc gcggtcatgc cgttgccgcg
     2881 tactcgggtc agctcgagac cgacgagcgc gaacaggtcg aagaccgcct gcggcgcaac
     2941 gagatcaagg ccgtggtctc gacgtcggcg ctcggcatgg gctacgacaa gcccgacctc
     3001 gcgttctgcc tgcacgtcgg ctcacccgcg tcgccggtcg cgtattacca gcaggtcggc
     3061 cgcgccgggc gagccctcga caccgcggtc gctgtcttgc tgccggccga gaccgacgag
     3121 cgggtctggg cctacttcgc cgacgcatca acgcccgacc ctgcagttgc gcagcgggtg
     3181 ctggcgacct tgaacgagtc gggcgagccg ctgtcgttgc ccaagctgga ggactccacc
     3241 ggcgcacgtc gtggtcggct cgagtcactg ctgaagatcc tcgcggtcga cggcgcgata
     3301 cgccgtgtag gcggcggctg ggaggcgacc ggcgaaccgt gggtgttcga cgacgagcgc
     3361 tacgccgcat tgggcgcggc acgtgccgcc gaggccggca tcatgcgcag ctatgcggcc
     3421 ggggccggct gcttgatgtc gttcctgcgg ttgtcgctcg acgatcccca cgcaggcgag
     3481 ccgtgcggcg tctgctcggt gtgcaccggc gcgttgcctg cgggtctggc ttcaaagcca
     3541 acgacggccg acatcgatgc cgcgcgcgtc ttcatgcgtg gggtcgatgt cgtgatcgag
     3601 ccgcgcaagc tctggccttc cgggggggcc cgcaaggggc ggatcggcac cgtactcgct
     3661 gaggggcgag cgcttgcgtt cgccgacgat cccggctggg ccgacctcgt cgtccggctt
     3721 actcgtgaag gcgcacccga tgccgagttg ccgtcagagg tcgccgccgg cgtcgtcgaa
     3781 ttgctcgcgc ggtgggctcg ccagtggccg gagcgtccaa ccgcaatcgc tgcgatcccg
     3841 tctcgtgcgc atccgttgct tgtcgcgtcg ctggcccgtc acattgccga ggtcggccac
     3901 ctcgagctgc tcgcgccgtt cgagatcgac gggcctgtgc cggtgtcgga ggttgcgtcg
     3961 gcgcagcggg tcgccgcggt cgcgtcgagc ctgcgcttgg cggctggcgt ggagatgcca
     4021 cccgggccga tcttgctcgt cgacgacgtt tacacgtcgg gctggaccat gacggtcgcc
     4081 gcggcgatgc tcggcgaggc aggagcggcg gcggtctatc ccctggtgct gcaccggcgg
     4141 ccatagggtt gcgtcacgga ctgtagatct ccaggaggca acacatggcc gacgacgaca
     4201 tcctcgcgcg catctccgac ctcgtcgctg aagaacacca cctcaccggc accgagggcg
     4261 ctgctgatcg ccgtcggcag atcgaagagc aactcgacca gtgctgggac ctcctgcggc
     4321 agcgacaggc caagcgtgag ttcggcgaca accccgacga ggcgcacgca cgaccgatcc
     4381 ccgaggtcga gcactaccag cagtagcccg gccgcgaacc gcagagccga tccgaaccac
     4441 ccgcgccaag actgccccta gggcatcacg acgccttgca acaccacggg tccgagggtg
     4501 tagtcgagtt cggtcggctg caagacgcca gccggggtga ccgtgtccat caccttcggc
     4561 accgtgttcg gatcggccgt gcatagcggc gtggccaacg cggtcgcaca cacgccgaaa
     4621 ttgaacggct gcggcgtggc agcgaaggtg cgggcattgt cctgaccgaa tccgtcttgg
     4681 cccgtcagcg cgatcgtgaa cgcccagccc gacgacggcg tcccgcccag cgccgtcttg
     4741 tcgacgctga acgtggtcca cctcgagatc gagttggcgc tgatcgtcac cgtgccctga
     4801 gtggtacccg aagcatcgac gaacgcctgc ccgaagccct gcacctcgat cagtctcgac
     4861 cacgcgtcgg cggcggcgat cgagtagtgc cgcgacgcga atgccgccga cgtcgacgtc
     4921 gtggtagccg ccgggttgtg cacgtacagg tcgaccaatt gcgcgccgtt cgtcgagccg
     4981 aacgtcgacg tcaaatcgcg agtctgcacg cggaacgtca ccgtcgtgcc ggtgtcgtag
     5041 acctggaact gttcgaggtc gaatgcaccg ggcacgaaat tgctggacgt cgggtacgcg
     5101 tagttgcccg gtccgttgtc gtcgttgtcg gggtcggttg cggtgaacag caacgtcccg
     5161 ttgacgacgt cattgatgag ggtgaccgtg gtctgcgcgg tcgcgcccga cgccgtcatc
     5221 gcggtgacga ccagcacgtt cgtgcccgcc gccaccgtca ccggcatggt gaaactgccg
     5281 tcgagtgcag cggtcgtgct gaccagtgtg gtcgcgtcgt tgttgtcagt cgcgacatca
     5341 gcaatgacga ccttcgcgct cggtgcggtc gtgccgatca ccggaactgt gccggtggcg
     5401 gcggtgttgt ttccgggcga ggtgaccgtc agcgtcgtgg ccgcctgggt gtgggtcacg
     5461 taccgcgcga tggtcgacga cggcttttcg acgacccgac cggccgcgag gtcagcggtc
     5521 agccgcacct gcgcggccgc accccacgtg agcggagcgg cagagccgtc gggttgcccg
     5581 tcggtgtagc cgatcgatgc gacagtcggg tccgtgccga agggggacgc cgcgacgttg
     5641 gcgaaatccc aatcctgctc aggcacgagc ccgacgccac cagccagctg accgatgctc
     5701 atcagcagat cggcggccgt tgctgactgc cccgttgcga gatcttgttc cccgcgctcg
     5761 cccgagagca ccggccagat gtgcccggtg ccttggccac tgggcgccca cggcgtaccg
     5821 gtcgtgccgc aactcgtgag cggctcgggc gcgtagcagt cgccgtagcc gtcgccgttg
     5881 tagcgcaacc accccgggcc actgggcgtc gtccgctcaa ttgtcgcgtc gacgacggag
     5941 agtgaattcg cgacgaccgg gtcggacgcc gacagctcac cgagcctgac cagctcgagg
     6001 aatccggcgt cgatgacaga acgctggtcg aggtcagggc cgccgttgcc gacgttgtag
     6061 gtgatcgccg cgttgggatc gcccgtcttc gagagcctga tgaagtacgg cgacgacgac
     6121 agcggacctg tggtcgtgac gtcccagctc ttgatgctgc gctggtagag gtcggcggtc
     6181 gccaggtaga cctgcgcgtc ggccgagtcg ccgtgctgct gcgcaatcgt gccggcggcg
     6241 acgagacccg cgatctcggc ggcgatcgtc gatggcgaat aaccactctg ctcttcccac
     6301 cgctcgacac cgaacgccgg gccgtgactg acgaggaagt tcgccgccgc cttgatgtgc
     6361 ggccacagcg ttgcgtcgtt gccaagcccc gattgctgcg ccatcaggat cgggtacgcc
     6421 gcctcgtcga gttgagtgtt gaacgagtcg ggagcgacct tgccgttgag caaggagttc
     6481 cttggcatcg agccgtccgg caattgctgg cgcaagaaca ggaaccgaac ggcgtccctc
     6541 gcagtattga tgtcgccatc ggtgaacagc gcggtccacg cttcgtagag gtcgcgcgag
     6601 aagacctcgc ggtacgaacc gaagtaggta ttcgcaggat caccggccga aacggcctga
     6661 ccccacggac tcgtgagcga ggcggcgatg gctccaggga aagtcttgtc ttcgctcgcc
     6721 ttcacgacgt tcgccgacac gtagtacgca gcggccgttt ggcgggcctg ggcgttcgtg
     6781 agcccggcaa ggtgcgtggc gggtgcgcgc aagccgtcgt cgtaccgctg ccactgcgac
     6841 tcgtacaccg caagtgctcc gagatagttc tggctggacg acattgcagc ggtgtggacg
     6901 gcggcacttt gggtcgtgcc gaatccgagc gcgatcgtcg tcgagttgaa cagaccgagg
     6961 ttcacgcgtg ctgtctgctc gacgttgccg ctcgtcgcat ctgtgtaagt cggcgacagc
     7021 gtgtgcgtgg catcaagctc ggcaagaccg tcactcggtg tgccgacgaa tccgctgctc
     7081 acggcggtga aaggccggtc ggcgtgtaac gccagataag tcggcaccgc gtagtcgcgg
     7141 ttcgctgcgt tcgtcgcggt cacggtgtcg aacgagacgg gcaccggttg cccggtcgag
     7201 gtgtctgtgg tcgcggagtc ggcgccgccg ttgcccgtgc cgccgccgcc attaccgccc
     7261 gccgttgcgt cgaggcgcac gtagagctgg tagtgccggg cggcagccgt aaacgggagg
     7321 aattgcgtgt gcatgatgac gctgtcgcgc gccgggtcgg tcaggtaggt cgtacgcagc
     7381 tggtagccgt ggctgttcga cgtcgacacg atcgtgcacg ccataccgct gcggtcggct
     7441 gaggcggtgt aggtggtgtc gcggctttgc aggtcggtga aggccgatcc gtcgctgacc
     7501 aagaactgca ttgtctcgac gttcgtggtg tcgacggtgg gtgcgtacac atctgagagc
     7561 acgccgttcg cgacggtgta ccagatcttg gacgtcgtgt tgcgcgcggt gcccagacag
     7621 tccttgcgtg attggtcgaa gtgactcaga gcacccggcg caccaggagc ttgcgtgacc
     7681 ggaggtggca ccgccccggc agtcgttgct tggaggccgg cggccgccaa c
//
LOCUS       DASWVF010000084         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_231576, whole genome shotgun sequence.
ACCESSION   DASWVF010000084 DASWVF010000000
VERSION     DASWVF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231576"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VGD55_01480"
     CDS             complement(<1..1311)
                     /locus_tag="VGD55_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0869041.1"
                     /translation="MAATVTQQRLLIFWVPDGLVPEWFWHATPGPLTIRSDRAQDTDL
                     TGTTFNNDIPAADRPTFVLQPLAPYANQILLPKGINSAGSNDHGLAVESTLTGEEIQA
                     KNTAGGPSIDAIMAAANKTSNHIVSAFRTGCYVKQTKLSTAGHPYRDTAGAAMIEISS
                     SPTTDARVMLDAVSGRATASTGTGTTTTTSTASADALKAKTRIAALGAVKSRVDAMSC
                     AAGNAAGQRLEAYIANVDDLETRETAISTGMVTVTKTTTPFSISSILNPSDATMKADE
                     SDIKHLPDIAPFVRELVVTSLALDYAPAVAMQWGATGINKVSGDTLTDYRYDFIPTFE
                     NKGAGEHGLAHPDNNAGFGGSSHVITGASSTRDRVRDRRWLFGQLRLLLDRLSSIPDG
                     SGTLLDNTTVLYASEFGGPFANSTAGQHSTSNLPFMLIGGANSPF"
     gene            complement(1428..>2830)
                     /locus_tag="VGD55_01485"
     CDS             complement(1428..>2830)
                     /locus_tag="VGD55_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0869042.1"
                     /translation="VPYTRLTRVEYKATVNAAFGITPDVSGLPNDSRVGPFTSNVGSP
                     DPADEFLLASEDLAAQIVPAKLPTCTGATAATCVTTNYQAPIERLYRRSLTAAEVTNL
                     ANMISPLEAAGVTSQDATRAMVLSTLLSADFLFRSAPLSGDAARGRRLTEHLGYALWD
                     APPDPTLVAAGNVTAAQLGASLRTQALRLGGDAKAVPVLARFLAQWLRVDSDDKLDDP
                     NLNFAASPLYAELQAFVQNALTKNVPVTSFINGTQGFIQKSNFAAYAMTPVTSTATVV
                     PVTWSASSVRRGILGEEVFMDATRNPDPGRRPIFRGHLVRSSLLCQPIASPPAELVAL
                     NSEVTDRTTDTRCKACHSLMDPIGQAFAPNDLDNTIGAPAPIVNGGGEVTGTFKDLPT
                     LMDAIAGSQAYADCFSRNLLAFFLEQDPDYVDAASVSDVSAVVKSGGGLADAIGQAVV
                     SLEKRSQSTTPWCTGQ"
BASE COUNT          436 a          887 c         1002 g          505 t
ORIGIN      
        1 gaacggcgag ttggcgcccc caatgagcat gaacggcagg ttcgacgtcg agtgctgtcc
       61 ggccgtgctg ttggcgaacg gcccgccgaa ctcggaggcg tacaacacgg tcgtgttgtc
      121 cagcaacgtc ccgctgccgt cgggaatgct cgacagccga tccagcagca gcctcagttg
      181 accgaacaac caacggcggt cacgcacgcg gtctcgcgtg gagctggctc cggtgatcac
      241 gtgactgctg ccgccgaagc cggcgttgtt gtccgggtgc gcgagcccgt gctcgcccgc
      301 gcccttgttc tcgaaggtcg ggatgaagtc gtaccggtag tcggtcaagg tgtcgccgga
      361 caccttgttg atgccggtcg cgccccattg catcgccacc gccggcgcat agtcgagcgc
      421 cagcgaggtc accaccagct cgcgaacgaa cggcgcgata tcgggaaggt gcttgatgtc
      481 gctttcgtcc gccttcatcg tcgcgtcgct gggattgagg atggacgaga tgctgaaggg
      541 ggtggtcgtc ttggtcaccg tcaccatgcc ggtgctgatc gccgtctctc gcgtctcgag
      601 gtcgtccacg ttggcgatat aggcttccag gcgctggccg gcggcgttgc cggccgcgca
      661 gctcatggcg tcgactcggc tcttgaccgc gcccagcgcg gcgatgcgcg tcttggcctt
      721 gagcgcgtcg gcggacgccg tgctggtcgt cgtggtggtg ccggtgccgg tgctggcggt
      781 ggcccgcccg ctcaccgcgt cgagcatgac gcgcgcgtcg gtcgtcggcg acgaggagat
      841 ctcgatcatc gcggcgccgg ccgtgtcgcg gtacgggtgt cccgcggtgc tcagcttggt
      901 ctgcttcacg tagcagccgg tccggaaagc gctcacgatg tgattcgagg tcttgttggc
      961 tgccgccatg atggcgtcga tcgacggccc gccggcggtg ttttttgcct ggatctcttc
     1021 gccagtcagc gtcgactcga cggctagacc gtggtcgttc gaaccggcgc tgttgatccc
     1081 ctttggcagc aggatctggt tcgcatacgg cgccaggggc tgcaaaacga atgtcgggcg
     1141 atcagccgcc ggaatgtcgt tgttgaaggt cgtcccggtc agatcggtgt cctgtgcccg
     1201 atccgatcgg atggtcagcg gcccaggcgt cgcgtgccag aaccattccg gtacgaggcc
     1261 gtccggaacc cagaagatga gcaagcgctg ttgcgtgacc gtcgcggcca tcgcccgact
     1321 gatcaggcga tcccagagca gcgacgtggc cgcaacgcca gccaggctgg cgggcagccc
     1381 tttcatgaaa gtgcgccgag agtggagtgg gatcatgttc atcctcttca ttggcccgtg
     1441 caccacggag ttgtcgattg cgaccgcttc tcgaggctga cgacggcttg cccgatcgca
     1501 tcggccagac cgccgcccga cttcacgacg gctgacacgt cactgaccga cgccgcgtcg
     1561 acgtagtccg gatcctgctc gaggaaaaac gccaggagat tgcgcgagaa gcagtcggca
     1621 taggcctggc tgccggcgat ggcgtccatc agggtcggca gatccttgaa cgtccccgtc
     1681 acttcgccac cgccgttgac gatcggcgcc ggcgctccaa tcgtgttgtc gaggtcgttc
     1741 ggagcgaaag cctggccgat ggggtccatc aacgagtggc aagccttgca gcgggtgtcg
     1801 gtcgttctgt cggtgacctc cgaattgagg gcgaccagct cggcgggcgg cgacgcaatg
     1861 ggctgacaga gcagcgagga ccgcaccagg tggccgcgga agatcggacg gcggcccggg
     1921 tccgggttgc gggtggcgtc catgaacacc tcctcgccca ggatgccgcg ccgaaccgag
     1981 ctcgcgctcc aggtgaccgg cacgacggtg gcggttgatg tcaccggggt catcgcatag
     2041 gcggcgaagt tgctcttctg aatgaacccc tgcgtcccgt tgatgaaaga ggtcacgggg
     2101 acgttcttcg tgagcgcgtt ttgcacgaac gcctgcagct ccgcgtagag gggcgacgcc
     2161 gcgaagttca ggttcgggtc atcgagcttg tcgtcgctgt ccacacgcag ccactgcgcg
     2221 agaaagcggg ccagcacggg gacggccttg gcgtcgcctc ccagccggag cgcctgggtc
     2281 ctgaggctgg cgcccaactg ggccgccgtg acgttgcctg cggccaccaa cgtcgggtcc
     2341 ggcggcgcgt cccaaagcgc gtagcccaga tgctcggtca ggcggcgccc gcgggcggcg
     2401 tcaccgctga gcggagccga ccggaacaga aaatcggcgc tgagcagcgt cgaaagcacc
     2461 atggcgcggg tcgcgtcctg ggacgtcaca ccggccgcct ccaggggact gatcatgttg
     2521 gccaggttcg tgacctcggc cgcggtcaac gaacggcggt acaggcgctc gatgggcgcc
     2581 tggtagttcg tcgtgacgca ggtggcggcg gtggcgccgg tgcaggtcgg cagcttggcc
     2641 ggaacgatct gtgcggcgag gtcttcgctg gcgagcagga actcatccgc gggatcagga
     2701 gatcccacgt tcgacgtgaa ggggcccacg cgactgtcgt ttggcaggcc gctgacgtcg
     2761 ggcgtgatgc cgaacgcggc gttgacggtc gccttgtact cgacccgcgt gagtcgggta
     2821 taggggacgt
//
LOCUS       DASWVF010000085         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_180572, whole genome shotgun sequence.
ACCESSION   DASWVF010000085 DASWVF010000000
VERSION     DASWVF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180572"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGD55_01490"
     CDS             <1..879
                     /locus_tag="VGD55_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEY0869043.1"
                     /translation="MAAIEIIERIMAIRPTPILVCSTEGDAERAAAALAAGAIDVVAR
                     PTAKGADRTSYAEALRRGVRVASRVKVITHPRGRLKSRSTSTSAGWPVTSSTEGTIDL
                     IAIGASTGGPQALAHLLGALPADFGPAVVVVQHMADGFIEGLANWLDELCALPVGVAT
                     SGARLLPGTVMIAPSARNLIVNDHLRVSIEQPPASQFHVPGVDVTFESVARTIGPRAV
                     GVILTGMGRDGAIGLKAMRDAGAITIGQDEGSSAVWGMPAAAAACGAVQRQLPLIEIA
                     AELMSILEGDSFGGDR"
     gene            888..2390
                     /locus_tag="VGD55_01495"
     CDS             888..2390
                     /locus_tag="VGD55_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY0869044.1"
                     /translation="MTDDEFAMLRTILEKSAGLVHDETRRDSLNLAVAGRIEATGATS
                     VAAYLAMLGGVRGAPERQQLIDVVTIQETHFFRNPPQIRALRQHVLPELIRRSVAQNR
                     PLTIWSAGCSTGEEPYSLAMLVRELLPMATRAHVRIVGSDVSTNALAFAEAGRYGARA
                     VQMADPIDLERWFTYENDAYVVRDDVRELVDLRLHNLITDPPPFEAGEVDLLLCRNVT
                     IYFNRVTTKALMARFHQTLSDGGYLFLGHSETLWQLSDAFTLLPLGDAFVHRRDDGPK
                     GRVTLPDRRTEVGDAPILLPERRGRSERRRRLDETAKALAVPPLPTRTPPKGLLGRGL
                     DRPAEGRVPGGSKAPIAHSGDDFAASARTALADGRYTDAVVAAARAAKTDPMLVEPHL
                     IGGEALVNLGRDADAIKELRQAVYLQPASAPALLLLAGALDREGEPAAAARAYRSAAA
                     TVVDLPPEQVAGFFGGRDPGELVALCVRLAEQAERAAKTNKGPRYKGRET"
     gene            2390..2809
                     /locus_tag="VGD55_01500"
     CDS             2390..2809
                     /locus_tag="VGD55_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0869045.1"
                     /translation="MIGYIMFRLGDRRFACRLDEVREVVRLEGLEVLPGMTPPISGVL
                     ELRGNPLPVVDLRAGDVETRAGMRGDVLVMATGIDAMGVVVDQVTAVHDEAELISLGE
                     ERPSGLPDYVVEVLRHRGATHTPVLLVDLRAMLSVAV"
     gene            complement(2806..>3562)
                     /gene="disA"
                     /locus_tag="VGD55_01505"
     CDS             complement(2806..>3562)
                     /gene="disA"
                     /locus_tag="VGD55_01505"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEY0869046.1"
                     /translation="ERTAKQTGYPVISVSQSMRIIALYVGGRRYVLDDSGAILSRANQ
                     ALATLERYKLRLDEVSGTLSALEIEDLVTVRDAAAVAQRLEMVRRIASEIEGYVVELG
                     TDGRLLSLQLDELLAGVEPERELVVRDYLPSLTGKKVRTPDDVLHDFDALTAAELLDL
                     STIARVMGYAAGADALDAPVSPRGYRLLAKVPRLPNLVVDRLVDHFGGLQKLLAAGID
                     DLQAVEGVGETRARSVREGLSRLAESSILERYV"
BASE COUNT          574 a         1162 c         1209 g          617 t
ORIGIN      
        1 atggcagcaa tcgaaatcat cgaacggatc atggcgatcc ggccgacgcc catcttggtg
       61 tgttcgaccg agggggacgc cgaaagagcg gccgcggccc ttgccgccgg cgcgatcgat
      121 gtcgtggcga gacccaccgc caaaggtgcg gaccgaacct cctacgccga ggcgctacgg
      181 cgtggggtcc gggtggcttc gagggtcaag gtcatcacgc atccgcgcgg tcgtctgaag
      241 agccgaagca cgtcgacgtc ggctggctgg cctgtcactt cgagcaccga aggcaccatc
      301 gatctcattg cgatcggtgc gtcgaccggt ggaccgcagg ccctcgcgca tttgcttggg
      361 gcgctacccg cagacttcgg gcccgcagtg gtcgtcgtcc agcacatggc cgatggattc
      421 atcgaaggcc tcgcgaactg gctcgacgag ttgtgcgcgt tgccggtcgg tgtggcgacg
      481 tccggtgcgc ggctgttgcc agggaccgtg atgatcgcgc cgagcgcccg caacctcatc
      541 gtgaacgacc acttgcgcgt cagcatcgag cagccgccgg cgagccagtt ccacgtgccc
      601 ggcgtcgacg tgaccttcga gagcgttgcg cgcacgatcg gtccgcgcgc ggtcggcgtc
      661 atccttaccg gtatgggccg tgacggcgcg atcggcctga aggcgatgcg cgacgcgggc
      721 gcgatcacca tcggccagga cgaaggttcg agcgccgtgt ggggaatgcc ggccgccgcg
      781 gccgcgtgcg gcgcggttca acgccaactc ccgttgatcg agattgccgc cgaactcatg
      841 tcgattctcg agggcgactc tttcggtggt gaccgatgag gtcatcgatg accgacgacg
      901 agttcgcgat gctgcgaacg atccttgaga agagcgcagg tctggttcac gacgagacaa
      961 ggcgcgactc actcaacctt gcggtggcgg gccgcatcga ggcgaccggc gcgacgtcgg
     1021 tagctgctta cctcgcgatg ctcggcggcg tgcgcggcgc accagagcgt cagcagttga
     1081 tcgacgtcgt gacgattcag gagacgcact tcttccggaa cccgccgcag atccgagcgt
     1141 tgcgccagca cgtgctgccg gagctgatcc gccgctcggt agcgcagaac cgcccgttga
     1201 cgatttggag tgcggggtgc tcgacaggtg aagagccgta cagcctcgcg atgctcgtgc
     1261 gcgagctgct gccgatggcc acccgcgcgc acgtgcgcat cgtgggttct gatgtctcga
     1321 ccaatgcgct ggcgttcgcc gaagcgggca ggtacggagc gcgcgccgtt cagatggctg
     1381 accccatcga tctcgagcgc tggttcacgt acgagaacga cgcctacgtc gttcgcgacg
     1441 acgtgcgtga actggtcgat ttgcggttgc acaacctgat caccgatccg ccgccgttcg
     1501 aagcgggcga ggtcgacctg ttgctctgcc gcaacgtcac catctacttc aaccgcgtga
     1561 caaccaaggc gctcatggcg cgctttcatc agaccttgtc ggacggcggc tacctgttcc
     1621 tcggtcattc cgagaccttg tggcaactca gcgacgcgtt cacgttgctg ccgctcggcg
     1681 atgcgttcgt ccaccgccgc gatgacggcc ccaagggtcg ggtcacgttg cccgatcgcc
     1741 gcaccgaagt tggcgacgct ccgatcctgc tgccggagcg tcggggtcgc agcgagcgtc
     1801 gccgccgcct cgatgagacc gccaaggcat tggccgtgcc accgcttccg acacgcacgc
     1861 cacccaaggg gttgcttggt cggggcctcg acaggccggc agagggccgt gttcccggcg
     1921 gctccaaggc tcccatcgcg cattctggcg acgatttcgc tgccagcgct cgcactgcgt
     1981 tggccgacgg gcgttatacc gatgctgtcg tcgcggccgc ccgtgcagcc aagaccgatc
     2041 ccatgctcgt cgagccgcac ctcatcggtg gcgaggcact ggtgaatctg ggccgcgacg
     2101 ccgacgcgat caaagagttg cggcaggccg tgtacttgca acccgcgtcc gcacccgcgc
     2161 tgttactgct tgcgggcgcg ctcgatcgtg agggtgagcc cgctgctgcc gcccgcgcgt
     2221 accgatcggc tgcggcaact gttgtcgacc tgccgccaga gcaggtggcc ggcttcttcg
     2281 gtggccgcga ccctggcgag ctcgtcgcgt tgtgcgtgcg actcgccgaa caggcggagc
     2341 gcgcggccaa gaccaacaaa ggcccgcgtt acaagggacg ggagacgtga tgatcggcta
     2401 catcatgttc cgcctcggcg atcgccggtt tgcctgccgg ctcgacgagg ttcgcgaggt
     2461 ggtacgcctt gaaggactcg aggtcttgcc gggcatgacg ccaccgatca gcggcgtgct
     2521 cgaactgcgc ggcaacccgc taccagtcgt cgacctgcgt gctggcgacg tcgagacccg
     2581 cgccggcatg cgcggcgatg tactcgtgat ggcgaccggg atcgacgcga tgggagtggt
     2641 cgtcgatcaa gtgacggccg ttcatgacga ggccgaactg atttcactcg gcgaggagcg
     2701 accgtcgggg ttgcccgatt acgtcgtcga ggtgttgcgc catcgcggtg ccacgcacac
     2761 gccggtgctg ctggttgacc ttcgcgcgat gctgtcggtc gctgtctaga cgtagcgctc
     2821 gaggatcgac gactctgcca atcgtgagag gccctcgcga acgctgcgtg ctcgcgtctc
     2881 gccgacccct tcgaccgcct ggagatcatc gataccggcg gccagcagtt tctgtagccc
     2941 gccaaagtgg tcgacgagac gatcgaccac gaggttgggt agccgcggca ccttggccag
     3001 cagtcggtag ccgcgcgggc tgaccggagc atcgagcgca tcggctccgg cggcgtagcc
     3061 catcacccgc gcaatcgtgc tgaggtcgag aagttcagcc gcggtaagtg cgtcgaagtc
     3121 atgtaagacg tcgtcgggcg tgcgcacctt cttgccggtc aacgagggca ggtagtcacg
     3181 aacgacgagt tcgcgctcgg gctcgacgcc ggccagcagc tcgtcgagtt gcaagctgag
     3241 caggcgaccg tcagtgccga gctcgacgac gtaaccctcg atctcactcg cgatccgccg
     3301 caccatctca aggcgctggg cgaccgcggc cgcatcgcgc accgtgacga gatcctcgat
     3361 ctcaagcgcc gacagggttc cgctgacttc gtcgaggcgc aacttgtagc gctcgagagt
     3421 cgccaacgcc tggttggcgc gcgacaggat cgcgccggag tcgtcgagca cgtaccggcg
     3481 gccgccgacg tagagcgcga tgatgcgcat cgactggctc accgagatga cggggtagcc
     3541 ggtctgtttc gcggttcgtt cg
//
LOCUS       DASWVF010000086         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_282622, whole genome shotgun sequence.
ACCESSION   DASWVF010000086 DASWVF010000000
VERSION     DASWVF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282622"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /gene="pstA"
                     /locus_tag="VGD55_01510"
     CDS             complement(<1..606)
                     /gene="pstA"
                     /locus_tag="VGD55_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0869047.1"
                     /translation="MSSAAMAGPGAAATSQDWKVRGRRAVVNRVMTAAMYLAFIIAVI
                     PLALVIWYTVGKGLARFSYSFLTHSMAGVGPNDIDGGIYHAIVGTVEQLLIAAVISVP
                     LGLLVAIYLNEYGAKGFSTVVRLLVDVMTGIPSIVAGLFVLAFWVIGLHEGYSGFAGS
                     LALAILMLPVVTRSSEEMLRLVPNSLREAGYALGIPKWRVIL"
     gene            complement(603..1325)
                     /gene="pstC"
                     /locus_tag="VGD55_01515"
     CDS             complement(603..1325)
                     /gene="pstC"
                     /locus_tag="VGD55_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0869048.1"
                     /translation="MLSSVIALVLAIPVALGVAIFVVEYAPRRLATWLGYLIDMLATV
                     PSVVYGLWGLIFLVPHIRGFQHFLATKFGWIPIFDDPNNRTSVASRSIFVASVVLAIM
                     VLPVIAAISREVLRQVDPNLKEAALALGATRWEMVRIALLPPSRPGIIGGIMLGLGRA
                     LGETIAIALVLGTSFAVSIHILQPGGNTIAANIANYFGEAGATGRSALIATGLVLFIM
                     TLGVNLIARTVIYRAAVKQGAR"
     gene            1356..>1781
                     /locus_tag="VGD55_01520"
     CDS             1356..>1781
                     /locus_tag="VGD55_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0869049.1"
                     /translation="MFGIAVLVFGTVLSSVIALVLAIPVALGVAIFVVEYAPRRLATW
                     LGYLIDMLATVPSVVYGLWGLIFLVPHIRGFQHFLATKFGWIPIFDDPNNRTSVASRS
                     IFVASVVLAIMVLPVIAAIAREVLRQVDPNLKEAALALGA"
BASE COUNT          361 a          571 c          548 g          301 t
ORIGIN      
        1 caggatcacc cgccacttgg ggattcccag ggcgtagcca gcctcgcgca gcgagttggg
       61 caccagcctc agcatctctt ccgacgatcg ggtgaccacc ggaagcatca ggatcgcgag
      121 cgccaacgag ccggcgaacc cggagtagcc ctcgtgcaac ccgataaccc agaaagccag
      181 cacaaacagg ccggcgacga tcgaagggat acccgtcatg acgtcgacga gcaaccgcac
      241 aacggtggag aaacccttag cgccgtattc gttgagatag atggccacca gcagcccgag
      301 gggcaccgag atcacggccg caatcaacaa ctgctcgacg gttccgacga tcgcgtggta
      361 gatgccaccg tcgatgtcgt tcggaccgac gcccgccatc gagtgtgtca ggaacgaata
      421 gctgaagcgc gccaagccct taccgaccgt gtaccagatc accagtgcga gcggaatgac
      481 cgcgatgatg aacgcgagat acatcgccgc ggtcatcacg cggttgacga ccgcacgacg
      541 accgcggacc ttccagtctt gcgatgtcgc agccgcgcct gggcccgcca tggccgccga
      601 actcaccgcg caccctgctt cacggcggca cggtagatga ccgtgcgggc aatcaggttg
      661 acgccgagcg tcatgatgaa cagcactagg ccggtcgcga ttaatgctga gcgaccggtc
      721 gcgccggctt cgccgaagta gttggcaata ttcgccgcga tcgtgttgcc gcctggttga
      781 agaatgtgaa tgctcacggc gaagctagtg ccgagcacca aggcaatggc gatcgtctcg
      841 ccgagtgcgc gcccgagccc gagcatgatg ccgccaatga tgccggggcg actcggtggc
      901 agcaacgcaa tccgcaccat ttcccagcgg gtcgcaccca gcgccagcgc ggcttctttg
      961 aggttcgggt cgacctgccg caagacctca cgcgagatgg cagcgatgac gggcaacacc
     1021 atgatggcca gcaccacgga ggctacgaag atcgagcgac tcgcgaccga agtccggtta
     1081 ttgggatcgt caaagatcgg tatccagccg aacttcgtcg ccaggaaatg ctggaaccct
     1141 ctgatgtgcg gcaccaagaa aatgaggccc cacaagccgt agaccaccga tggcaccgtg
     1201 gccagcatgt cgatgagata gccgagccag gtagcgagcc gcctcggggc gtattcgacg
     1261 acgaagatgg cgactcccaa cgccaccgga atggcgagca ccagggcgat gacgctggaa
     1321 agcacggtgc cgaaggcaag gacggcgatc cgccgatgtt cggcattgcc gttctcgtct
     1381 tcggaactgt gctgtccagt gtcatcgcgc tggtactcgc gatcccggtc gcgttgggtg
     1441 tggccatctt cgtcgtcgag tacgcgccac gccgcctcgc gacctggctc ggctatctca
     1501 tcgacatgct ggcgactgtg ccgtcagtcg tttacggcct gtgggggctg atctttctgg
     1561 tccctcatat ccgcggcttc cagcatttcc tggccacgaa gttcggctgg atccccatct
     1621 tcgatgatcc gaacaaccgc acgtcggtcg ctagccgatc tatcttcgtc gcctcggtgg
     1681 tgctcgccat catggtgttg ccggtgatcg ccgcgatcgc gcgcgaggtg ttacgacagg
     1741 tcgacccgaa cctcaaggaa gccgcccttg ccttgggcgc g
//
LOCUS       DASWVF010000087         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_2286, whole genome shotgun sequence.
ACCESSION   DASWVF010000087 DASWVF010000000
VERSION     DASWVF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VGD55_01525"
     CDS             <1..347
                     /locus_tag="VGD55_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEY0869050.1"
                     /translation="YLLRAAKYHGEATDEPCPVCRKEPLTRVTYIYGDALGQYSGRIK
                     ATRELVELEYEVSEFTVYAVEVCQNCGWNHLRESYVLGHGGASSRGERAHARAAAGGR
                     RVTHSRERALSE"
     gene            400..1851
                     /locus_tag="VGD55_01530"
     CDS             400..1851
                     /locus_tag="VGD55_01530"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY0869051.1"
                     /translation="MDDGAAAVSSTGVIPPTHDDPFVRGGSAAFGGPLGRHAGSGWSW
                     WTPLRVLIAIAIASSVLGYLQKLPCRNQPWQGGFQYSHACYNDIYPLYFGEGLADGKR
                     PYLDAHADSNGQLTGEVEYPVLIGAAMDVGAQVDKLLYPKSDRGQGFLDVTWLMLMIA
                     AVVMVVATALTARRRIWDAAMVAAAPALVLAGLINWDLLAVALTSLAMLAWSRKNNFV
                     AGALLGLAIATKFYPLVFFLPLLMLCWRARQMRAFWVAVGGAAFAWVVVDVPIWIASP
                     EGFARFYYFSKTRGADWGSIWYISEKMFTWNLGTPSLNLYEAGAIAIAFLGVCWLALA
                     ATRRPRLPQLCFLTLALFLVFNKVYSPQYVLWLLPLVVLARPRWRAFLLWQAAEIIYF
                     LAIWMYLLDAPSGRAGKGLAWQPYAVAVGIRDVAVLALCALVVLDILHPDGDIVRADG
                     SDDPAGGVLDGAPDRFDREPEPYESDDAVPVPA"
     gene            complement(1875..2651)
                     /locus_tag="VGD55_01535"
     CDS             complement(1875..2651)
                     /locus_tag="VGD55_01535"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY0869052.1"
                     /translation="MNTLLPKIGAHVDQEDPLAEAVAREADLVQFFLGDPQGWKAPEI
                     RAGVDYFKNSPVDLYVHSPYVLNVATTNNRIRIPSRKLLTEHADAAASIGAKALIVHG
                     GHVLKDDDPKTGIENWRKTFERWTPPLPILIENTAGGDNAMARYFDRVAMLWDAVGEF
                     GPGFCLDTCHAHAAGEDLSTAVDRIMAITGRIDLVHANGSRDVFGSGADRHSNFEDGE
                     IDPALIVDIVRQAGTPVVCETPGGVAGQGADIAYLRKHLG"
     gene            2848..3138
                     /gene="rpsF"
                     /locus_tag="VGD55_01540"
     CDS             2848..3138
                     /gene="rpsF"
                     /locus_tag="VGD55_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0869053.1"
                     /translation="MRRYELMVLLDPDVEEKTVAPSLDQFLGVVRQDGGTVEKVDVWG
                     RRRLAFEISKKTEAIYAVIDIVAEPASVKELDRQLNLNETVLRTKLMRPEKH"
     gene            3141..3623
                     /locus_tag="VGD55_01545"
     CDS             3141..3623
                     /locus_tag="VGD55_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0869054.1"
                     /translation="MAAGDTVITLVGNLVDDPELRFTPNGQAVAKFRIASTPRFMDKA
                     TNEWKDGESLFLTCNVWRQAAENCAESLTRGMRVIVQGRLKQRSYETKEGEKRTVFEV
                     EVDEVGPSLRSATAKVIKAARGPGGGGGGGFGGGSGGDDPWASPAGGGGGSLSDEPPF
                     "
     gene            3763..3999
                     /gene="rpsR"
                     /locus_tag="VGD55_01550"
     CDS             3763..3999
                     /gene="rpsR"
                     /locus_tag="VGD55_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0869055.1"
                     /translation="MPKVPLRKPKKKVCVFCKEKVSYVDYKDTNLLRKYISDRGKIRA
                     RRVTGNCTQHQRDIATAVKNSREVALLPYTSAAR"
     gene            4014..4463
                     /gene="rplI"
                     /locus_tag="VGD55_01555"
     CDS             4014..4463
                     /gene="rplI"
                     /locus_tag="VGD55_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0869056.1"
                     /translation="MKLILTGEVTGLGAPGDIVEVKDGYGRNYLVPQGLAMRATKGAE
                     KQIASLKRARDIREVRDLAHAREIKTQLESLKVTLPTRAGAAGRLFGSVTVADVVAAV
                     KAAGGPILDKRKVELESPIKTLGSHQVVVRVQGDISAKVALEVTAAS"
BASE COUNT          720 a         1419 c         1554 g          825 t
ORIGIN      
        1 cctacctgct ccgggcggcc aagtatcacg gcgaggcgac cgacgagccg tgcccggtct
       61 gccgcaagga gccgctcacc cgagtgacct acatctacgg cgacgcgctc gggcagtaca
      121 gcggccggat caaggccact cgtgagcttg ttgagctgga gtacgaggtc agcgagttca
      181 cggtgtacgc cgtcgaggtc tgccagaact gcgggtggaa ccacctgcgc gagtcgtacg
      241 tgctcgggca cggcggggcc agcagccgtg gcgagcgggc tcacgcgcgt gctgccgccg
      301 gtggtcgcag ggttacccac tcccgcgagc gcgcactttc cgagtagcac ccgcgggggg
      361 tgtgcacctc gcgagggtcg gccgactaag tttcacttca tggacgacgg ggctgcagcc
      421 gtgagttcga cgggggtcat tccaccgacg cacgacgacc cgttcgttcg cggcggcagt
      481 gcggctttcg gtggtccgct cgggcggcac gccggctcag ggtggtcgtg gtggacccca
      541 ctccgcgtgc tgatcgcgat cgccatcgcg agctcggtgc tcggctacct ccagaagttg
      601 ccgtgccgca accagccatg gcagggcggc tttcagtaca gccacgcctg ctacaacgac
      661 atctatccgc tgtacttcgg cgagggcctt gccgatggca agcggcctta cctcgacgcg
      721 cacgccgaca gcaacgggca gttgaccggc gaggtcgagt accccgttct catcggtgcg
      781 gccatggacg ttggcgccca ggtggacaag ctgctgtatc cgaagagcga tcgcggccag
      841 ggcttcctcg acgtcacgtg gctgatgctg atgatcgccg cggtcgtcat ggtggtggcg
      901 accgcgctca ccgccagacg ccggatctgg gacgccgcga tggtcgcggc cgcgccggcg
      961 ctggtgctgg ccgggctcat caactgggac ctactcgcgg tcgcgctgac gtcgcttgcg
     1021 atgctcgcct ggtcgcgcaa gaacaacttt gtcgccggtg cgctgctcgg gcttgccatc
     1081 gcgacgaagt tctacccgct cgtgttcttc ctaccgttgc tgatgttgtg ctggcgggcc
     1141 cgtcagatgc gggcgttctg ggttgccgtc ggtggagccg cgttcgcgtg ggtcgtcgtc
     1201 gatgtaccga tctggatcgc gtcgcctgaa ggatttgcgc ggttctacta cttcagcaag
     1261 acgcgcggcg ccgactgggg ctcgatctgg tacatctccg agaagatgtt cacctggaac
     1321 ctcggcacgc ccagcctgaa cctctacgag gcgggcgcga ttgcgatcgc gtttctcggc
     1381 gtgtgctggc ttgcgttggc cgccacgcgg cgtccgcgct tgccgcagtt gtgcttcttg
     1441 acgctcgcct tgttcctggt cttcaacaag gtctactcac cgcaatacgt gctgtggctg
     1501 ttgccgcttg tcgtgctcgc gcgacctcgg tggcgggcat tcctgctctg gcaggccgcg
     1561 gagatcatct acttcttggc gatctggatg tacctgctcg acgcaccgag tgggcgggcc
     1621 ggtaagggct tggcctggca gccctacgcg gtcgccgtcg gtatcaggga cgtcgcggtg
     1681 ctggcgctgt gtgcgctcgt ggtgctcgac atcttgcatc cggacggcga catcgttcgg
     1741 gccgacggaa gtgacgatcc ggcgggcgga gtgctcgatg gcgcgccgga ccggttcgat
     1801 cgcgagccgg agccctatga gtctgacgac gcggtgccgg tgccggcctg aggttggatc
     1861 tgtcgggcgt ggcctcagcc gaggtgctta cgtaggtagg caatgtcggc gccttggccg
     1921 gccacccctc ccggggtctc gcagaccacg ggcgtgcccg cttggcgcac gatgtcgacg
     1981 atcagcgctg ggtcgatctc gccgtcctcg aaattcgaat ggcgatcggc acccgaaccg
     2041 aacacgtcgc gcgagccgtt ggcatgcacg aggtcgatgc gcccagtgat ggccatgatg
     2101 cggtcgaccg ccgtcgagag atcctcgccg gcggcgtggg cgtggcaggt gtcgaggcag
     2161 aagccgggac cgaactcgcc gaccgcgtcc cacaacatcg ccacccggtc gaagtaccgc
     2221 gccatggcgt tgtcgccacc cgcggtgttc tcaatcagga taggcaaggg cggggtccag
     2281 cgctcgaacg tcttgcgcca gttctcgatg cccgtcttcg ggtcgtcgtc tttcaggacg
     2341 tgcccgccat ggacgatcag ggctttcgct ccgatgctcg cggccgcatc ggcatgctcg
     2401 gtcagcagct tgcggctcgg gatgcggatg cggttgttcg tggtcgccac gttcaggacg
     2461 tagggcgagt gcacgtagag gtcgacgggt gagttcttga agtagtcgac gccggctctg
     2521 atctcggggg ctttccagcc ttgggggtca ccgaggaaga actgcacgag gtcggcttca
     2581 cgggcgaccg cctcggctag ggggtcctcc tggtcgacgt gggcgccgat cttcggtagc
     2641 agggtgttca cgggtttcac cttaggccgg gtggcggaca cttgctctgg gtgccgcccc
     2701 cctggtccag taggcgtccg accgggctgt ggacggattt cgggttgggc cgcaggcctt
     2761 ggtactgtga cacgttgctt cgacgcaacg agccctcctg tcacggatcg accgtggccg
     2821 cccagaccga aggaggtcgg tgcacgcatg cgtcgttacg aactgatggt gcttctcgat
     2881 cccgatgtgg aagagaagac cgtcgccccc tcccttgacc agttcctcgg tgtcgtgcgc
     2941 caagacggcg gcactgtcga gaaggtcgac gtgtggggcc ggcgtcgctt ggcgttcgag
     3001 atctccaaga agaccgaggc tatttacgcg gtcatcgaca tcgtcgccga gccggcgtcc
     3061 gtgaaggagc tcgaccgtca gctgaacctg aacgagaccg tgcttcgcac caagctcatg
     3121 cgtccggaaa agcactagcc atggctgcgg gagacaccgt catcacgctg gtcggcaatc
     3181 tggtcgacga tcccgagttg cgattcaccc ccaacgggca ggccgtcgcg aagttccgca
     3241 tcgcttcaac gccgcgtttc atggacaagg cgacgaacga gtggaaggac ggcgagagcc
     3301 tgttcctcac gtgcaacgtc tggcggcagg cggccgagaa ctgcgccgag tcgctcacgc
     3361 gtggcatgcg ggtgatcgtg cagggccgcc ttaagcagcg gtcgtacgaa accaaggaag
     3421 gcgagaagcg caccgtcttc gaagtcgagg tcgacgaggt cggcccgtcg ctgcgtagcg
     3481 caactgccaa agtcatcaag gccgcgcgcg gtcctggtgg cggtggcggt ggcggcttcg
     3541 gcggtggctc tggcggcgat gacccgtggg caagtcctgc cggtggtggt ggcggcagtc
     3601 tttctgacga gccgccgttc taagagaact tcgctggacg gcgctgtgcc gacgctttgc
     3661 tggtgacgtc ccgcatacaa aaccctgact gcaacaacta actgcaaaag ctgactgccc
     3721 gggcgaaagc cacacgggcg ctgagattct tggagcaacg cgatgccgaa agttcccctg
     3781 cgcaagccga agaagaaggt ttgcgtgttc tgcaaggaga aggtctccta cgtcgactac
     3841 aaagacacca acctgctgcg gaagtacatc tccgaccgcg gcaagatccg ggcccgtcgg
     3901 gtcaccggca actgcaccca gcaccagcgc gacatcgcca ccgcggtgaa gaacagccgc
     3961 gaggtggcgt tgctgcccta caccagcgct gcgcgctaga agacgggacg accatgaagc
     4021 tcatcctcac aggcgaggtc actggcctcg gtgcccccgg cgacatcgtc gaggtcaagg
     4081 acggctacgg ccgcaactac ctcgtgccgc agggtctcgc gatgcgcgcg accaagggcg
     4141 ccgagaagca gatcgcgtcg ctgaagcggg cccgtgacat ccgcgaagtt cgcgacctcg
     4201 cgcacgcgcg ggagatcaag acgcagctcg agagcctgaa agtcacgcta cccacgcgtg
     4261 ccggtgctgc tggccggctg ttcggttcgg taactgtcgc tgatgtcgtg gcggcagtga
     4321 aggcggccgg cggcccgatc ctcgacaagc gcaaggtcga actcgaatcg cccatcaaga
     4381 cgctgggtag ccaccaggtc gtggtgcgcg tgcagggcga catctcggca aaggtcgcgc
     4441 tcgaggtcac cgccgcctcg taggttcggc tggttccgca ggttcggctg gttcggccgg
     4501 ttcggccggg tttagtcg
//
LOCUS       DASWVF010000088         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_2291, whole genome shotgun sequence.
ACCESSION   DASWVF010000088 DASWVF010000000
VERSION     DASWVF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VGD55_01560"
     CDS             <1..707
                     /locus_tag="VGD55_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869057.1"
                     /translation="AQVLDGLALLKPCFSAPGSCAGCTCDENAANILNTGAAPENQHT
                     TKQFGGDPSVTYHVKLRVVGVAERYWYAGGTLDPTSKIFYTGGLPTIHMPPQAPNSNL
                     GPGQGACKIHPPQTDSSYPIPFTVPPDVAPADNCYNGFNIFAMAVASPKQSYFLNYTT
                     DYDNVDRQPHAVYKTDYTVTIPISGQSKIDFYVIDGDHHEVANTTMTVPNVKNTAIKQ
                     PYVGNFLELEVVDVTR"
     gene            complement(715..>1716)
                     /locus_tag="VGD55_01565"
     CDS             complement(715..>1716)
                     /locus_tag="VGD55_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869058.1"
                     /translation="TIAGQTAPGEGIGIMAREVSFNGATNDIVRYVRFRQGDLDPDSK
                     KSGINLLDASNMIFDHVSIEFAQWNNIDSVGASNITVQSSIDANPIGQQFAAHTETGP
                     YTWYRNLFANAHNRNPLAKANTEYINNVIYDFQLGYTAGNTAGTFSHDLVNNYFVTGP
                     ATTNAGDALFQVDNQPIYNAGNLLDANGDGVLNGSPLASPASTVERSSPWGPNTAGIP
                     TVSAAAAFASVLADAGASLHRDQVDTMVVADARSLGKTGSLWTHQTDTKLTNSGYGTL
                     AGGTPPADADGDGMPDAWEARYGLNPHDASDATGDFDRNGYTNVEKYINGLVDGSYP"
BASE COUNT          294 a          566 c          548 g          308 t
ORIGIN      
        1 cggcgcaggt gctggacggg ctcgcgctgc tcaagccgtg cttttccgcg cctggaagct
       61 gcgcggggtg cacgtgcgac gagaacgccg cgaacatcct gaatacgggc gccgcccccg
      121 agaaccagca caccacgaag cagttcggcg gggatcccag cgtgacctac cacgtcaagc
      181 tgcgcgtcgt cggcgtggcc gaacgttact ggtacgcggg cggcaccttg gacccgacca
      241 gcaagatctt ctacacgggc gggttgccca ccattcacat gccgccgcag gcgccgaaca
      301 gcaacctcgg cccgggccag ggtgcctgca agatccaccc gccgcagacc gacagctcgt
      361 acccgattcc gttcacggtc ccaccggacg tcgcgccggc ggacaactgt tacaacgggt
      421 tcaacatctt cgcgatggcc gtcgcgtcgc cgaaacagag ctacttcctg aactacacga
      481 ccgactacga caacgtcgac cgacagccgc acgccgtcta caagacggac tacacggtga
      541 ccattccgat ctcgggtcag tccaagatcg acttctacgt gatcgacggc gaccaccacg
      601 aggtcgcgaa cacgacgatg accgtcccca acgtcaagaa cacggcgatc aagcagccct
      661 acgtcggcaa tttcctggag ctggaagtcg tcgacgtcac ccgctgagca caggctacgg
      721 gtacgagccg tcgaccaggc cgttgatgta cttctcgacg ttcgtgtagc cgttgcgatc
      781 gaaatcgccg gtcgcgtcgg aggcgtcgtg cgggttcaga ccgtatcgcg cttcccacgc
      841 gtccggcatc ccgtcgccgt ccgcgtccgc cggcggcgtc ccgccggcca gcgtcccgta
      901 gccgctgttg gtgagcttgg tgtccgtctg gtgcgtccag aggctgcccg tcttgcccaa
      961 cgatcgagcg tcggcgacga ccatcgtgtc gacctgatcg cggtgcagcg acgcgcccgc
     1021 gtcggcaagc acgctggcaa aagcggcggc cgccgagacg gtggggatgc cggccgtgtt
     1081 cggcccccac ggcgacgacc gctcgaccgt cgaggcgggc gacgccagcg ggctgccgtt
     1141 cagcacaccg tcgccgttcg cgtccagcag gttgcccgcg ttgtagatgg gctgattgtc
     1201 gacctgaaaa agtgcgtcgc cggcattcgt cgtcgccgga ccggtgacga aatagttgtt
     1261 cacgagatcg tgcgagaagg tcccggctgt gttaccggcc gtgtagccca gctggaagtc
     1321 gtagatcacg ttgttgatgt attcggtgtt ggccttcgcg agcgggttgc ggttgtgggc
     1381 gttcgcgaac aggttgcggt accacgtgta agggcccgtc tccgtgtgcg cagcgaattg
     1441 ctgcccgatc ggattcgcgt cgattgagct ctggacggtg atgttgctcg cgcccaccga
     1501 gtcgatgttg ttccattgcg cgaactcgat cgagacgtgg tcgaagatca tgttgctggc
     1561 gtccagcagg ttgatgccgc tcttcttcga gtcgggatcc aggtcgccct gacgaaaccg
     1621 gacgtaccgc acgatgtcgt tcgtcgcgcc attgaacgag acctcacgcg ccatgatgcc
     1681 gatgccctcg cccggcgcgg tctgcccggc gatcgt
//
LOCUS       DASWVF010000089         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_78773, whole genome shotgun sequence.
ACCESSION   DASWVF010000089 DASWVF010000000
VERSION     DASWVF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78773"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VGD55_01570"
     CDS             complement(<1..999)
                     /locus_tag="VGD55_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869059.1"
                     /translation="MGLQPMKILRWLAAPLAGLLLAALAACNGNAVVTLTSTPSTDTF
                     FAYRVGLVSVQLRTSSGSSSVNVLPAGTTVDLARLVNLSEVIGASSGKTGTFKQAVVT
                     LDYSSAQIIYDNGTPEGVQLAPMGASGQALGRVTLTLLLDPTNELSIAAKSTTRLSLD
                     FNLAASNVVNLSAKTVSVMPMMAASSLPIDDKTVRIRGPIGTVNGGTTSGTYSASIAP
                     NSWYSTGITPFDFSTAEGGSLRVTPTTGTTYEINGTPVSGTTGLTQLASLSAGTMVEA
                     FGTMTTSTSTSNSLFNNNNVGTPTETCADGTVPQTVNGVLSCTDGSTLESTSTTSGT"
     gene            complement(1173..>2342)
                     /locus_tag="VGD55_01575"
     CDS             complement(1173..>2342)
                     /locus_tag="VGD55_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0869060.1"
                     /translation="GFVMNTAVAGAYESPWMNEELAIFRDAAARFIESEMVPNEAEWR
                     KQQNVGKEIWRKAGALGLLCTDVSAEYGGAGGDFRHEAVIYEELSRRGVSGFGQGVHS
                     ICAHYLVNHGTEEQKRRLLPRMASGELIGAIGMTEPAAGSDLQGVKTRAVRDGDSYIV
                     NGSKIFITNGYLAELIALVVKTTPGEGAKGTSILMVETKDLPGYRVGRILDKMGLKAQ
                     DTAELFFENVRVPAANLLGGTEGQGFYQLMGDLPYERALIAVVGVGAMEGAVTETARY
                     VKERKAFGRSIAEFQNTKFKLAEIATKTRVARVFVDDCIAKIVAGTLDTVTASMAKYW
                     VTDTQQEVLDECVQMHGGYGYMNEYLVCRMFVDSRVQRIYGGTNEIMKELISRSI"
BASE COUNT          385 a          769 c          730 g          458 t
ORIGIN      
        1 ggtgcccgac gtggtgctgg tcgactcgag cgtcgagccg tcggtgcagc tgagcacgcc
       61 gttgacggtc tgcggcacgg tgccgtccgc gcaggtctcg gtcggcgtcc cgacgttgtt
      121 gttgttgaag agcgagttcg acgtgctggt cgaagtcgtc atagtgccga aggcctcgac
      181 catggtgccg gcgctcaagg aggcaagctg ggtaagaccg gtcgtgccgg atacgggagt
      241 gccgttgatc tcgtaggtgg ttcccgtggt cggtgtgacc cgcagcgacc cgccctccgc
      301 ggtgctgaag tcgaacggcg tgatgccggt tgagtaccag ctgttcggcg cgatgctggc
      361 ggagtacgtg ccgctggtcg tgccgccgtt gaccgtgccg atcggcccgc gaatgcgcac
      421 cgtcttatca tcgatgggca gcgagctggc cgccatcatc ggcatcaccg acaccgtctt
      481 cgcgctcagg ttgacgacat tggaggcggc caggttgaag tcgaggctca ggcgcgtggt
      541 gcttttggcc gcaatgctca attcattggt cgggtcgagc aataaagtga gggtcacccg
      601 gcccagggcc tggccgctag cccccatcgg cgccaactgc actccctcgg gtgtgccgtt
      661 gtcgtagatg atttgcgccg agctgtagtc gagcgtgacg acggcctgct tgaacgtgcc
      721 ggtcttcccc gacgacgcac cgatcacctc gctcaagttc accaggcgag caagatcgac
      781 ggtggttccc gccggcagca cgttgaccga cgagctaccg ctcgaggttc gcagctgcac
      841 cgataccagg ccgacccgat aagcaaagaa tgtgtccgtc gacggcgtcg aggtcaaagt
      901 gaccaccgcg ttgccgttgc aggcggcgag cgcagccagc agcaggccgg cgagtggggc
      961 cgcgagccat cggagaatct tcatcggctg taatcccatc gcagcatggt cagcctattc
     1021 taccggcatc ggaagcgccg attgagcaaa gttctgtgaa gtaatccggg aaacagcaca
     1081 gcgtcgcgtt cctgcgacag ccggcgcggg cgcttgcccg tagcttgggc gtcgcaaggc
     1141 cgtttccggc gccgccgcag cgcgaccgcg cgctagatcg agcgcgaaat caattccttc
     1201 atgatctcgt tggtgccgcc gtagatgcgc tgtacacgcg aatccacgaa catgcggcac
     1261 accaggtact cgttcatgta gccgtatccg ccgtgcatct gcacgcactc gtcgagcacc
     1321 tcctgctgcg tgtcggtgac ccagtacttg gccatcgagg cggtcacggt gtcgagcgtg
     1381 ccggcgacga ttttcgcgat gcagtcatcg acgaacacgc gcgccacgcg cgtcttggtg
     1441 gcgatctcgg cgagcttgaa cttggtgttc tgaaattcgg cgatgctccg gccgaaggcc
     1501 ttgcgctcct tcacgtagcg cgccgtctcc gtaactgcgc cttccatggc gccgactccg
     1561 accacggcga tcagcgcgcg ctcgtacggc aagtcgccca tcagctgata gaacccctgt
     1621 ccctcggtgc ctcccaggag gttggctgcg ggtacacgca cattctcgaa gaacagctcc
     1681 gccgtgtcct gcgccttcag tcccattttg tcgaggatgc gtcccacccg atagcccggt
     1741 aggtccttgg tctccaccat cagaatcgat gtgcccttcg cgccctcgcc cggcgtcgtc
     1801 ttcacgacga gcgcgatcag ctcggccaga taaccgttgg tgatgaatat tttcgagccg
     1861 ttgacgatgt agctgtctcc gtcgcgcacg gcacgcgtct tcactccctg caaatccgag
     1921 cccgccgccg gctcggtcat gccgatcgct ccgatcagct cgccgctcgc catgcgcggc
     1981 aggagcctgc gcttttgttc ctcggtgccg tgattgacga gatagtgcgc gcagatgctg
     2041 tgcacgccct ggccgaaacc gctcactccg cggcgtgaga gctcctcgta gatcaccgcc
     2101 tcgtgccgaa agtcgccgcc cgccccgccg tactcggcgc tcacgtcggt gcacaaaagg
     2161 cccaaggcgc cggccttgcg ccagatctcc ttgccgacgt tctgctgctt gcgccactcg
     2221 gcctcgttcg ggaccatttc cgactcgatg aagcgagccg cggcatcgcg aaatatcgcg
     2281 agctcctcgt tcatccacgg tgactcgtag gcgcccgcaa ccgctgtgtt catgacaaat
     2341 cc
//
LOCUS       DASWVF010000090         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_206283, whole genome shotgun sequence.
ACCESSION   DASWVF010000090 DASWVF010000000
VERSION     DASWVF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206283"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGD55_01580"
     CDS             <1..325
                     /locus_tag="VGD55_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869061.1"
                     /translation="ATAVKFVQWLTSPTVNLQFAIATGDLPLRQSETTLPDYAKFLST
                     YPSDKVFVDNLNNVKHVRPNITSYAQVSTAIGQMVQAVLLGQKQPQAALDAAKSQVES
                     ALAGQ"
     gene            445..1290
                     /locus_tag="VGD55_01585"
     CDS             445..1290
                     /locus_tag="VGD55_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0869062.1"
                     /translation="MVSPSVLLLAVFGMVPVVWAFVLSFQRNDLQTPARWVGFHNYQE
                     LIHDPIFRDSIRHTIVYTVIFVPITLVLSLVVAGALNRKIRFITLYRLAVFVPVVTST
                     VATGVIFTWLLDPQYGIVNGTLHKVGLPTSGFFSSPHDALFAIVAMTVWGWVGFGALI
                     FLAGLQSVPRELIEAAQIDGCSKTTAFWRIQVPLLRPVSAFLVVWLTINALQLFDEVY
                     VTTKGGPLDSTTVVVYYLYQQAFAYFHAGYAAAIAVVLFAAIAIVTVVQLRLARDPAA
                     KATDR"
     gene            1287..>1623
                     /locus_tag="VGD55_01590"
     CDS             1287..>1623
                     /locus_tag="VGD55_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0869063.1"
                     /translation="MTTLTRSRRPSPWHLVLAPLAALMLVPLVWMLITSLSTEAETRR
                     FPPGLPATLRWGNFRRAWDQAPFGHWLLNSAVVSGTCVISNLVLCSLAGYAFARINFA
                     GRQLAFFAML"
BASE COUNT          274 a          535 c          482 g          332 t
ORIGIN      
        1 cgccacagcg gtcaagttcg ttcaatggct gacctcaccg accgtcaact tgcagttcgc
       61 gatcgccacc ggcgacctgc cgctgcggca atcggagacc acgcttccgg actatgcgaa
      121 gttcctgtcg acttaccctt ccgacaaggt gttcgtcgat aacctgaaca acgtgaagca
      181 cgtgcggccc aacatcacgt cgtacgctca agtctcgacc gctattggcc agatggtcca
      241 agcggtgctg ctgggccaaa agcagccgca agcggccctc gatgcggcca agtctcaagt
      301 tgagagcgcc ctggcgggtc aatgagtcaa gcaattcggc tcccgtcccg ccgaccagcg
      361 ggcggccgcc gagcggcgat cctgccgtcg tcagcccgtc gacgcgaccc gcggagacga
      421 gctgaccaat tcgcgggctg gggcatggtc agcccaagcg tgctgctgct tgctgtattc
      481 ggcatggtcc cggttgtgtg ggcgttcgtc ttgtcgttcc agcgaaacga cctgcaaacg
      541 cccgcgcgat gggtgggctt ccataactac caggaactga tccacgatcc gatctttcgt
      601 gactcgattc gccacacgat cgtctacacc gtcatcttcg tgccgatcac gctggtgctt
      661 tccctagtcg tcgctggagc gctgaatcgc aagatccgct tcatcaccct ctatcggcta
      721 gccgtattcg tacccgtcgt cacgtcaacg gtcgcgacgg gtgtgatctt cacctggctg
      781 ctcgatccgc aatacgggat cgtcaacggg acgctgcaca aagtcggact gccaacgtcg
      841 ggcttcttct cgtcacctca cgatgcacta ttcgccatcg tcgcgatgac ggtctggggc
      901 tgggtcggtt tcggggcgct gattttcctg gccggcctgc aatccgtccc gcgtgaactg
      961 atcgaggcag cgcagatcga cggctgctcc aagacaacgg cgttttggcg catccaagtg
     1021 ccgctgctgc gcccggtctc agcgttcttg gtggtctggc tgaccatcaa cgcactgcag
     1081 ctcttcgatg aggtgtacgt cacgaccaag ggcggtccgt tggactccac gacggtcgtc
     1141 gtctactacc tctaccaaca ggccttcgct tacttccacg ccggctacgc agcggcgatc
     1201 gctgtcgtct tgttcgcggc aatagccatt gtcacggttg tgcagctgag gctcgcgcgt
     1261 gacccagccg ccaaggcaac ggaccgatga cgaccctcac ccggagcagg cgaccatcgc
     1321 cgtggcatct ggtgctcgcg ccactggcgg cgctgatgct cgtgccactg gtgtggatgc
     1381 tgatcacgag cctgtcgacc gaggccgaga cgcgcagatt tccacccggg ctgccggcga
     1441 cgctgcgatg gggcaacttc cgacgggcct gggaccaagc gccgttcggc cactggctgc
     1501 tgaacagcgc cgtcgtgtcg ggcacgtgtg tcatcagcaa tctggtcctg tgcagccttg
     1561 ccggctacgc cttcgcgcgt atcaacttcg caggcaggca gctcgcgttc tttgcgatgc
     1621 tcg
//
LOCUS       DASWVF010000091         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_410131, whole genome shotgun sequence.
ACCESSION   DASWVF010000091 DASWVF010000000
VERSION     DASWVF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410131"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1818
                     /locus_tag="VGD55_01595"
     CDS             262..1818
                     /locus_tag="VGD55_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0869064.1"
                     /translation="MADIFDDYVLAQAWDEMFEMPGVPRPAYEVLFAALQPLESDDLN
                     FRAEQLSRMFTDRGVTFAMGGEERPFPLDLVPRVIMASEWDAVTRGVTQRVRTLEAFL
                     ADIYGAGRVLQDGIVPRSLITSSIHMHRAVHGFDPPNGVRVHVAGIDLVRDEAGAFRV
                     LEDNVRVPSGVSYVIENRRAMTRSIPAVFGEHRVHPVHEYPARLLTALRAAAPSGISD
                     PTVAVLTPGIYNSAYFEHALLARLMGVELLEGRDLVCRNNRLRMRTTDGERPVHVLYR
                     RVDDEFLDPLHFRSDSQMGCPGLINAARAGNVTIANAVGNGVADDKLLYTYVPELVRY
                     YLGEEPVLDNVETYRLEDPEILHDVLGRLHELVIKPVDGSGGKGIVIGPQATEQTLAE
                     LHDVVESNPRGWIAQRPVALSTAPTLIGDRLVPRHIDLRPFAVNDGNEVWVLPGGLTR
                     VALPEGALVVNSSQGGGSKDTWVLADEAQRVPPQLAADLPSLTELPERASDPVTGVRD
                     SGPSVQMAQQ"
     gene            1832..>2222
                     /locus_tag="VGD55_01600"
     CDS             1832..>2222
                     /locus_tag="VGD55_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0869065.1"
                     /translation="MLSRIAESLFWIGRYVERADDTARLLDAFLRRVLEDPWTNEDAE
                     CRSLLAILGVPAPRDVVQHTGEIMRKVAFDDKTPTAIAGAVSAARENARGAREAISSE
                     MWECLNVTWRTMPERRRAAARLGPNTYL"
BASE COUNT          377 a          733 c          736 g          376 t
ORIGIN      
        1 atcgccagcc gggggcgcga cgcgatgcga tcaacagcgt gcaggaagcg gctctcagga
       61 gcgtgcggct tggccggtcg cccgtccggc tcggcaacgt ccatgcctgc tggcgcctcg
      121 cggttcatgc cttgctccta cccgctcgca cgctgggcca agcgaggtca gtctgagtcc
      181 tgggtcacgc tgcgtggccc ggtagcgctc ccgacgcgtg tccgtaacgc gcgcgacatc
      241 acttattcct aggatctggg gatggcagac atcttcgacg actacgtgct cgcacaggcg
      301 tgggacgaga tgttcgagat gcccggcgtg ccgcggccgg cgtatgaggt actgttcgcc
      361 gcgcttcagc cactcgaatc tgacgatctg aacttccgcg ccgagcaact gtcgcgcatg
      421 ttcaccgacc ggggagtgac gttcgcgatg ggtggtgagg agcggccttt cccgcttgat
      481 ctcgttccga gggtgatcat ggcctcggaa tgggacgccg tcacccgcgg cgtgacccag
      541 cgggtgcgca ccctcgaggc attccttgcc gacatctacg gggcggggcg ggtgctgcaa
      601 gacggcatcg tcccgcgctc gctgatcacg tcctcgatcc acatgcatcg ggcagtccac
      661 ggattcgacc cgccaaacgg cgtccgcgtt catgttgcag gtattgacct cgtgcgcgac
      721 gaggccggcg cgttcagggt tctcgaagac aacgtgcggg taccgtccgg ggtgagctac
      781 gtcatcgaga accgccgcgc gatgacgcga tcgatccccg cagtgttcgg tgagcaccgg
      841 gtacatccgg tgcacgaata tccggccagg ctgctcacgg cgttgagggc ggctgcaccc
      901 tcggggattt ccgatccgac cgtcgctgtg ctcacccccg gtatctacaa ctcggcgtac
      961 ttcgaacacg cgctgctcgc gcgcctgatg ggcgtcgaac tgctcgaggg gcgcgacctc
     1021 gtctgccgca acaacaggct tcgcatgcgg acgacggacg gtgaacgtcc agtgcacgtg
     1081 ctctatcgac gggtcgacga cgaattcctc gacccactgc acttccgcag cgattcgcag
     1141 atgggctgcc cgggcctgat caacgcggcg cgagctggca acgtgacgat cgcgaacgcc
     1201 gtgggtaacg gagtggccga cgacaagctg ctgtacacct acgtgcccga gctcgtgcgc
     1261 tattacctgg gcgaggagcc ggtgctcgac aacgtcgaaa cgtaccggct cgaggatccc
     1321 gagatcctgc acgacgttct cgggaggttg cacgagttgg tgatcaagcc ggtcgacggt
     1381 tcgggtggca agggcatcgt gatcggaccg caagccaccg agcagacgct tgccgaactg
     1441 cacgatgtcg tcgaaagcaa cccgcgtggc tggatcgcgc agcggccggt ggcgctgtcg
     1501 acggctccga cgttgatcgg cgaccggctg gtgccgcgac acatcgacct gcgcccgttc
     1561 gcggtcaacg acggcaacga ggtctgggtg ttgccaggtg ggctcacccg agtcgcgctc
     1621 cccgaaggcg cgctcgtcgt caactcaagc cagggtggcg gctcgaaaga cacctgggtg
     1681 ctggccgacg aagcgcaacg tgttccgccg cagctcgcgg ccgacctacc ttcgctgacg
     1741 gaacttcccg agcgcgcgtc cgaccctgtc accggcgtcc gcgactccgg cccgagtgtg
     1801 caaatggcac agcagtgaac agcgtgcgca gatgctgagc cgcatcgccg agtcgctgtt
     1861 ctggatcggc cgttacgtcg aacgagccga cgacacggcg cgactgctcg acgcattctt
     1921 gcggcgggtg ctcgaggatc cctggaccaa cgaggacgcc gagtgccggt cgttgctggc
     1981 aattctcggc gttcctgcac cgcgagacgt cgtccaacac accggcgaga tcatgcgcaa
     2041 ggtcgccttc gacgacaaga caccgaccgc cattgccggc gccgtttcgg ccgcgcgcga
     2101 gaacgcacgc ggagctcgcg aagccatctc cagcgagatg tgggagtgcc tcaacgtcac
     2161 gtggcgcacg atgccagaac gtcgccgggc agcggcaagg ctcggtccca acacctacct
     2221 cg
//
LOCUS       DASWVF010000092         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_384695, whole genome shotgun sequence.
ACCESSION   DASWVF010000092 DASWVF010000000
VERSION     DASWVF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384695"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGD55_01605"
     CDS             complement(<1..261)
                     /locus_tag="VGD55_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869066.1"
                     /translation="MDTVANFLSSSCCDVWARVDGTSNVDGQSRSVLNGFEPVDLAVK
                     QIVDAPCPGNKFSQRYFLNDDAREGDACTRESQYDGLLFANAV"
     gene            complement(874..1446)
                     /locus_tag="VGD55_01610"
     CDS             complement(874..1446)
                     /locus_tag="VGD55_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869067.1"
                     /translation="MGRSKLSGLAFVGAALIAAISLGCSGPKAAPGTAGNARQMCVPD
                     QLTAEVSGPDGTGGAAEYRVTIRDDGVPCSLPSAPKSLTGVDASGRQIRLTSTPLPVD
                     DIAAMTTGRPADLTNKVRADVVLLTGIACPAAQPQAISTTPQFVSLRLGIGTGTLVVP
                     FGHGPNPANTTMSLPCGVEMSDFYASFLTG"
BASE COUNT          320 a          513 c          549 g          290 t
ORIGIN      
        1 gacggcgttg gcgaagagca ggccgtcgta ctgcgattcc cgcgtgcacg cgtcaccttc
       61 acgagcgtca tcgttgagga agtaccgctg gctaaacttg ttacccggac atggggcgtc
      121 aacgatctgt ttgaccgcca ggtctaccgg ctcgaagccg ttgaggacgg aacgcgactg
      181 accatcgaca ttcgatgttc cgtcgacccg cgcccagaca tcgcaacaac tcgagctcaa
      241 gaaattagcg acggtgtcca agactacctt tctcgcgttg cagccgcgat acgcgacggg
      301 tggaaggcga agccccgacc gccagacgaa gatgatgact agaaagtcgg gatgcgcgag
      361 ggcggctgcc cgaacaacgg tgcaactgac gatgatcggt aagcccgcgg ttgcctcgta
      421 gctgtcgccg ccggccacag ttgtctcgac acgcacacgc tggctgtcta ggccactaac
      481 gtcgcgcact catgccgcga tcacggtttg ttgctggcag cccgatcggg gctagcgaag
      541 aaggagaacc cctcgcacta ggcagacgac tgtcaggacg gcgtaaccga tgtagacgcg
      601 tttcggcagc ttcttgccgc tacggcgaga aaccctccgg agccagagag cgactggcgg
      661 gtaggtgagg gcaacagcca gaaagagcca gcccccgacg ctgtgagttg aacggctgcc
      721 agccgcgata tacgtcttga tcactcggac agggtagggg gcggccggcc agggcgagcg
      781 ccgcagggga cgcagggcgc ctcgaagcaa catccgcctt tgccgcggag ggcgtgtttc
      841 cgcagagtcc gaggctcgta catggcttgc gcactagccg gtcaggaatg acgcgtaaaa
      901 gtctgacatc tcaactccgc agggcagcga catcgttgtg ttggcggggt ttgggccgtg
      961 cccgaagggc acgaccaggg tgccggtgcc gataccgagt cgaagtgaca cgaactgcgg
     1021 ggtcgttgag atcgcctgcg gctgagccgc cgggcacgcg atgccggtga gcagcaccac
     1081 atccgcgcgc accttgttag tcagatcagc cggccggccc gtcgtcattg ccgcgatgtc
     1141 gtccactggc agcggcgtgc tggtcaacct gatctgtcgc ccggacgcgt ccacccctgt
     1201 cagggacttc ggtgctgagg gaagggaaca cggcacgccg tcgtcgcgga tcgtgacgcg
     1261 gtactcggcg gcaccgccgg tgccgtccgg acctgacacc tcggcggtca gctggtccgg
     1321 cacgcacatc tgtctggcat ttcccgcagt gcccggagcc gccttaggcc ctgaacaacc
     1381 gagtgagatc gccgctatca gcgcggcgcc gacgaacgcc aatcccgaca gcttcgaacg
     1441 acccataccg ctaggacgta acactcgact ggtccgttgc acggcccaga ccaacgcacc
     1501 ctttttgccg ctggtggtgc gcgtgcgtgc gaacagtggg tgcttgttga gattggtact
     1561 cgtagcatca catccagaag acgagcagag gtcgtgtcgg atagttggtg atgatgaggc
     1621 aagggcggct ctgggtctct cgcggtcggc ttgcgcgcgt attactcgcg tt
//
LOCUS       DASWVF010000093         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_53407, whole genome shotgun sequence.
ACCESSION   DASWVF010000093 DASWVF010000000
VERSION     DASWVF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53407"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2256
                     /locus_tag="VGD55_01615"
     CDS             <1..>2256
                     /locus_tag="VGD55_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869068.1"
                     /translation="RIKWQVTESTLIARETYELVQDSDYNGARTTDTGQVVAMFAITS
                     HFDIKRSYNPQTGEENNLITENVTDRPWYEREYFRVDWSQNLVTDAYTVDTLSQIGIF
                     GGVTWQPEAYYVSDPTDPNAPVFDQDNGYFDVTQKAFASPQTINTPFGTFPACFLPAD
                     FAGGTGPIANCNPTEVTLRLSFKLKTNTDFEPEDQTGTKQYAFGWFTVDRYGYDRNYG
                     VLDDDWHRFAAKYNLWQQSHVQGAQCAVDYWRDASGNVIDYSVNAQGQFLTDGSTGLP
                     IPDPKGKPFPGSAPGSNVHADANGNGTEDVCEFTDASGKLVNPGSRCDEFSRLCDLPL
                     YNRQTHKIPWYYGPQSDTSLFAATANALGQWNLAVKRATQIGKVVELRRVGLDPSSVI
                     NGKDAKGNAIDLTTEAGLIADAGHLVPDVFVLCHNPVAASDDSSCGQVGLVARVGDLR
                     YNMVNILPNPQVPSPWGIMVDADDPLTGEKVVTSVNEWGAVLDIASQSTEDLIRWVDG
                     EITDAQITSGQYLKDWVSGDKLGAGPYAQAPLSAQEIKTRLASVDLGLSNLVQLSAAA
                     QAQPHALRLLAAANQLGTSLGPSLDAKLEGTRQALIGSTWETQLVTPEWLQASGQAPT
                     TPVAGNATALAQASNLRGMNPKLSAWLNTQKNITMAQWGSCEVDEPEPDSLVGLARQA
                     VTLYPLPDPKDPEYPYHLQQRQAALHQWIREQFHIAVIAHEMGHSMGLRHNFPGSFDA
                     LNYHTQYWQLRT"
BASE COUNT          418 a          834 c          699 g          305 t
ORIGIN      
        1 cgcatcaagt ggcaggtcac cgagagcacc ctcatcgcgc gcgaaacgta cgagctcgtg
       61 caggacagcg actacaacgg cgcgcgcacg acggacaccg ggcaggtggt cgcgatgttc
      121 gccatcacga gccatttcga catcaagcgc tcgtacaacc cgcagacggg cgaagagaac
      181 aacctcatca ccgagaacgt gacggaccgc ccctggtacg agcgcgagta cttccgcgtc
      241 gactggtcgc agaacctcgt cacggacgcg tacacggtcg acaccctgtc gcagatcggc
      301 atcttcggcg gcgtcacgtg gcagccggag gcgtactacg tctccgatcc gaccgacccg
      361 aacgcgccgg tgttcgacca ggacaacggg tacttcgacg tcacgcagaa ggcgttcgcc
      421 tcgccgcaga cgatcaacac gccgttcggc acgttccccg cgtgcttcct ccccgcggac
      481 ttcgcgggcg ggacgggccc gatcgccaac tgcaacccga ccgaggtcac gctccgtctg
      541 tcgttcaagc tgaagaccaa caccgacttc gagcccgagg accagacggg gaccaagcag
      601 tacgcgttcg ggtggttcac ggtcgatcgg tacggctacg accgcaacta cggcgtcctc
      661 gacgacgact ggcaccgctt cgccgccaag tacaacctct ggcagcagag ccacgtgcag
      721 ggcgcccagt gcgccgtcga ctactggcgc gacgccagcg gcaacgtcat cgactactcc
      781 gtcaacgcac aggggcagtt cctgaccgac ggctccacgg gccttcccat ccccgacccg
      841 aagggcaagc cgttccccgg ctccgcgccc ggctcgaacg tgcacgccga cgccaacggc
      901 aacggcaccg aggacgtctg cgagttcacc gacgccagcg gcaagctcgt caaccccggc
      961 tcgcggtgcg acgagttcag ccggctctgc gacctgccgc tgtacaaccg gcagacgcac
     1021 aagatcccct ggtactacgg gccgcagtcg gacacgtcgc tcttcgccgc gacggccaac
     1081 gcgctcggac agtggaacct cgcggtgaag cgcgccacgc agatcggcaa ggtcgtcgag
     1141 ctgcggcgcg tcgggctcga cccgtcctcc gtgatcaacg gcaaggacgc gaagggcaac
     1201 gcgatcgatc tcacgaccga agctgggctg attgcggacg cgggacacct cgtgccagac
     1261 gtcttcgtcc tctgccacaa cccggtcgct gccagcgacg actcgtcctg cgggcaggtg
     1321 gggctcgtcg cgcgcgtcgg ggaccttcgt tacaacatgg tcaacatcct ccccaatccc
     1381 caggtgccct cgccctgggg gatcatggtg gacgccgatg acccgctcac gggcgagaag
     1441 gtcgtcacga gcgtcaacga gtggggcgcc gtgctcgaca tcgcctcgca gagcaccgag
     1501 gacctgattc gctgggtcga cggcgagatc accgacgccc agatcacgag cgggcagtac
     1561 ctgaaggact gggtcagcgg cgacaagctc ggcgcgggcc cgtatgcgca ggcgccgctg
     1621 agcgcgcagg agatcaagac gcgcctcgcc tcggtcgacc tcggcctctc caacctggtg
     1681 cagctctcgg ccgccgcgca ggcgcagccg cacgcgcttc gcctcctcgc cgcggcgaat
     1741 cagctcggca cgagcctcgg gccctcgctc gacgcgaagc tcgaaggaac gcgccaagcg
     1801 ctcatcggct cgacctggga gacccagctc gtcacccccg agtggctcca ggcttcgggc
     1861 caggctccga ccaccccggt cgccggcaac gcgacggcac tcgcccaggc gtccaacctc
     1921 cgcggcatga acccgaagct ctcggcgtgg ctcaacacgc agaagaacat cacgatggcc
     1981 cagtggggct cctgcgaggt ggacgagccc gagcccgact cgctcgtcgg tctcgcgcgc
     2041 caggccgtca cgctctaccc gctccccgac cccaaggacc cggagtaccc gtaccacctg
     2101 cagcagcggc aggcggccct gcaccagtgg atccgcgagc agttccacat cgcggtcatc
     2161 gcccacgaga tggggcactc gatgggcctg cggcacaact tccccggcag cttcgacgcg
     2221 ctcaactacc acacgcagta ctggcagctg cgcacg
//
LOCUS       DASWVF010000094         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_257478, whole genome shotgun sequence.
ACCESSION   DASWVF010000094 DASWVF010000000
VERSION     DASWVF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257478"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1003
                     /locus_tag="VGD55_01620"
                     /pseudo
     CDS             158..1003
                     /locus_tag="VGD55_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1229..>2267
                     /gene="purF"
                     /locus_tag="VGD55_01625"
     CDS             1229..>2267
                     /gene="purF"
                     /locus_tag="VGD55_01625"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0869069.1"
                     /translation="MPRGDGRLNHDLLAEDKGPQDACGVFGVWAPGEEVANLTYFALY
                     ALQHRGQESAGIAVSDGKKILVYKDMGLVSQVFNESNLATLKGDIAIGHARYSTSGSS
                     VWENAQPTFRSTAIGSIALAHNGNLTNTGDLAQLVEKAQADSGEIAFDRRPLRSTSDT
                     DLVTSLLASHPDRSLETAAVEVLRQVEGAFSFVFMDENTLYAARDRYGVRPLALGKLE
                     HGWVVASETAALDIVGAAYVREIEPGELLAIDENGPRSVRFAEPKPKFCVFEFVYLAR
                     PDSNLDGRSVHTTRVEVGRQLAREAPAVADLVMPTPDSGTPAAIGYAEESGIPYAAGL
                     VKNAYVGRTFIQ"
BASE COUNT          352 a          763 c          733 g          419 t
ORIGIN      
        1 gctgggcggg ctgctcaccg aactcggctg gcgatggacg ttcctgctgc ctgcgccgat
       61 tgccgcagcc ctcgtcgtcc tgggtcgata gtacgtcgca cgtgataccc acgctcgcgg
      121 gacaggtggc tacgacgtac ccggagccgt aacggcgacc gccgggatgt tgctgctcgt
      181 tcgcacggtc gtcactgcgc cgtcgtccgg ctggggatcg atcagtaccg tcggtggctt
      241 caccgtgagc gccgccttgc tcgcagcctt cgtgatgatc gagctgcgat ctccgcaccc
      301 gctcgttcgc ctcgggattc tgcggtcggc acgcctcgtt cgggcgaaca tcgccgccat
      361 ggcgttgttc ggctcctaca tcgggttcca gttcatcggc accctctacc tgcaatcgct
      421 gctcggctgg tcgtcgctgc acaccgcttt cgcgttcttg ccggccggac tgttcgtcgt
      481 ccttgcatca acgcggatcg cgccggtcat caaccgattc ggaacgccgc gactgctggt
      541 ggtttcgttc gccgtgctga ccgccggata cgcgctgttc ctgcgtcttg gtgcgaacgc
      601 ctcgtatgtg acgttgttgc tgccgacgat gttgcttctc ggcctcgcgt tcggcatcgg
      661 gttcccgtcg ctgaacgtgc aggcgacgag cggggtcgcg gacgatgagc aagggctcgc
      721 gtccgggctg ctcaacacct cgttccaact cggcggcgcc atcggcctcg cgatcatcac
      781 cgcggtcgtg acgagtcaca ccggcaccgg aactggctcc aaggcggagc tcgcgggctt
      841 ccgtccaggc atcggggtcg cgacaggcat cgcgctgctt ggcctgttgg tctcgataag
      901 cggcgtcgtc agcgagcgtc ggtcccgagt tgcggagacc acgacggacg gcgacggcgg
      961 accggctctc gaggagttcg cgctcgcagg ccaggacacc tagctcgggg gagcatcgag
     1021 ctaggtcggg ggacttcgca tcccgtgtgg tcgcggtggc agccgttatg accgattcgg
     1081 gcaaccacac gggatgtgaa ccccgagaac gtcggcgagg agccgggcga tccgctcggt
     1141 ttggcacgta cactgaaacc agtccccagc attcctcgat ccactactcc gctcactcct
     1201 ccgctactcc gcaaagggag cccagcaggt gcctcgtggc gacggccggc tgaatcacga
     1261 tctgctcgct gaagacaagg gtccgcaaga cgcctgtggc gttttcggcg tgtgggcccc
     1321 gggcgaagag gtggccaacc tcacctactt cgcgctttac gcactccagc accgtggcca
     1381 ggagtcggcg ggaatcgccg tcagcgatgg caagaaaatc cttgtttaca aggacatggg
     1441 cctggtttcg caggtcttca acgagtcgaa cctcgccacc ctcaaaggcg acattgcgat
     1501 cggccacgcc cgctactcca cgtccggctc atcggtgtgg gagaacgcgc agcccacgtt
     1561 caggtcgacc gcgatcggca gtatcgcgct tgcgcacaac ggcaacctga ccaacacagg
     1621 tgatcttgcg cagctggtcg agaaggcgca ggccgacagc ggcgagatcg cgttcgaccg
     1681 gcgaccgctc cggtcgacgt cggataccga cctggtcacc tcgctgctcg cgagccaccc
     1741 cgatcgcagc ctcgagaccg ccgcggtcga ggtgttaagg caggtcgagg gtgcgttcag
     1801 tttcgtcttc atggacgaga acacgctcta cgccgcccgc gaccgctatg gcgtgcggcc
     1861 gctcgcgctt ggcaagctcg agcacggctg ggtggtcgcg agtgagaccg ccgcgctcga
     1921 catcgtcggc gcggcgtacg tgcgcgagat cgaacccggc gagctgctag cgatcgacga
     1981 gaacggcccg cggtcggtgc ggttcgccga gccgaagccc aagttctgcg tcttcgagtt
     2041 cgtctatctc gcccgcccgg attcgaacct tgacggtcgc agcgtgcaca cgactcgtgt
     2101 cgaggtcggc cgccaactcg cccgcgaggc gccggccgtt gccgatctcg tgatgcccac
     2161 acccgactcc ggtacgccgg ccgccatcgg ctacgcggag gagtccggaa tcccttatgc
     2221 agcagggctt gtcaagaacg cgtatgtcgg ccgcaccttc atccagc
//
LOCUS       DASWVF010000095         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_282787, whole genome shotgun sequence.
ACCESSION   DASWVF010000095 DASWVF010000000
VERSION     DASWVF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282787"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1305
                     /locus_tag="VGD55_01630"
     CDS             166..1305
                     /locus_tag="VGD55_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HEY0869070.1"
                     /translation="MKAVVYRGPFKVAVEDVDDPRIEEPLDAIIKITTANICGSDLHP
                     YEGRVAMDAGMVLGHENMGIVVEVGPGVGRVKVGDRVSVPFNLACGTCRNCDAGLTSA
                     CLRANPSGHPGAGYGYPKMGPFWGGQAEYLRVPYADFNLLELPAGDEFESDFAMLSDI
                     FPTGYHATELAQVGPGSSVAIFGAGPVGLMATHSAILRGASQIFVVDKEHDRLALVEQ
                     LGATAVDLSAVDPTQVIMDGTDGFGVDSGVEAVGFQAHDADGHEHPEMVLDNLVAVVR
                     ATGHIGVVGVYEAKDPKAATEGAKEGRIGFNYGAAFEKGLSIRSGQCPVKKYNRHLRD
                     LIIHGKATPSVIVSHELSLAEAPGAYDKFDKRIDGYTKVLLHPAA"
     gene            complement(1436..>1757)
                     /locus_tag="VGD55_01635"
     CDS             complement(1436..>1757)
                     /locus_tag="VGD55_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869071.1"
                     /translation="HPLSRIVHLDVEATKLGHCTSDVGRTLLFSSLRRSTGTGAALRP
                     APSASWTVSRASGSSSDMYVNNMSAPSAGKRERNGATNPGITTRDDGPFDLEFARTLV
                     RLSP"
BASE COUNT          349 a          527 c          579 g          302 t
ORIGIN      
        1 gcaggccgtt tcggatcatg ccgaggccga ggagaccgag gagttcccga ccatccaatc
       61 ggagcggtcg gacgcgcaac gcaaagacct cggtgagcag ttcttggccc agttcgaagc
      121 cgccggcggc aagccgtaga cccttctacg tccaaggagt atgtggtgaa agctgttgtc
      181 tatcggggac ctttcaaggt cgccgtcgaa gacgttgacg atccccgcat cgaggagccg
      241 ctcgacgcca tcatcaagat cacaaccgcg aacatttgcg ggtcagatct gcacccctac
      301 gagggacgcg tcgccatgga cgccgggatg gttctgggcc acgagaacat gggcatcgtg
      361 gtcgaggttg gccccggagt aggccgagtc aaggtcggag accgcgtctc ggttccgttc
      421 aacctggcct gcggaacatg ccgcaactgt gatgcgggac tcacgtccgc gtgtctgcgg
      481 gcgaacccgt ccgggcaccc cggtgccggc tacggctacc cgaagatggg cccgttttgg
      541 ggcgggcagg cggagtatct tcgggtgccg tatgcggact tcaacttgct ggaactgcct
      601 gcaggcgatg agttcgagtc ggatttcgcg atgctgtcag acatcttccc aaccggctac
      661 cacgcgaccg agctggcgca ggtcgggcca ggaagttcgg tcgcaatctt cggcgcgggg
      721 ccggtcggcc tgatggccac gcacagtgcg attctgcgcg gtgcgtcaca gatcttcgtc
      781 gtcgataagg aacacgaccg gctcgcgctg gtcgaacaac tcggcgcaac cgccgtcgac
      841 ctttccgcag tcgaccccac acaggtcatc atggacggca ccgacggatt cggtgtcgac
      901 tccggcgtcg aagcggtcgg cttccaagcc cacgacgccg atggccatga acacccggag
      961 atggtgctcg acaacctcgt cgccgtcgtg cgcgcaaccg gccacatcgg cgtcgtcggc
     1021 gtgtacgagg ccaaagatcc gaaggcggca accgagggcg caaaagaggg ccgcatcggc
     1081 ttcaattacg gagcggcgtt cgagaaaggg ctcagcatca ggtcgggcca atgtccggtg
     1141 aagaagtaca accggcacct gcgcgacctc atcatccacg gcaaagccac gccgtcggtg
     1201 atcgtgtccc acgaactatc gttggcggaa gcgcctggtg cgtacgacaa gttcgacaag
     1261 cggatcgacg ggtacaccaa agtgctcctg catcccgccg cataactacc ggtgctgaat
     1321 tctacggact gttctcttgg agaggtcagg tgttctcttg gagcggtcat ggcaacgaaa
     1381 cccgccacat tgcaggaccc gccggctcgt agtgacgaca tgacgccgca gccgactacg
     1441 gcgagagtcg tacgagggtt cgggcaaact cgaggtcaaa ggggccgtca tcacgggtgg
     1501 tgattccggg attggtcgcg ccgttgcgct cgcgtttgcc cgccgaaggc gcggacatgt
     1561 tgtttacgta catgtccgaa gaggagccgg acgcgcgcga gacagtccag ctggccgaag
     1621 gcgcaggtcg tagggcagca cctgtgccgg tcgacctgcg aagagaagag aagagaagag
     1681 tgcgcccgac atcgctcgtg cagtgaccga gcttggtggc ctcgacatct aggtgaacaa
     1741 tgcggcttag cggatgg
//
LOCUS       DASWVF010000096         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_206504, whole genome shotgun sequence.
ACCESSION   DASWVF010000096 DASWVF010000000
VERSION     DASWVF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206504"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGD55_01640"
     CDS             <1..752
                     /locus_tag="VGD55_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0869072.1"
                     /translation="ARFMVEPNAANLAALLADPVATAQITMLTLAPELPGALEAIRTL
                     AAIGIVVSVGHSDATGAQVHEAVLAGARMVTHIFNAQRGFDHREPGVSGQALYEAAMT
                     IGLIADFEHVSPEACAITLNAAPGRVALVTDAVAPAGMPPGVYELGGQQIAISTAAPL
                     ARNLDGTVAGSATFLDQAVRNLVGFGRDITEVLCAATVVPADALHRPDLGRLAPGTRA
                     DIVWWSDDLEPLGTWVDGVQVYAANAATASL"
     gene            762..1400
                     /locus_tag="VGD55_01645"
     CDS             762..1400
                     /locus_tag="VGD55_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0869073.1"
                     /translation="MVSGTQLAAFAATSFVLIVIPGPSVLFVIGRALSLGRGPAIASV
                     VGNGLGVYVVAVLVAFGLGSLVQRSELAFVAVKIAGAAYLVWLGVQAIRHRRSLAAAL
                     GATATPMSRWRAVRQGFLVGFANPKALIIFGAVLPQFVDRTAGRVPEQMLLLSAVAVG
                     IALISDTVWVLAASGVRGWFASNPRRLAAVGGAGGVAMIAVGVSVATTGRHD"
     gene            complement(1405..2469)
                     /locus_tag="VGD55_01650"
     CDS             complement(1405..2469)
                     /locus_tag="VGD55_01650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0869074.1"
                     /translation="MQHTGPGELMAREIAEQPAMFNRILADGRADIARVASDISAAGI
                     RFVLLAARGTSDHAALYTKYLVEIVLGLPAGLASPSTMTAYGARPDLHDVLFIAVSQS
                     GGSPDLVQSLTTARACGALTLAVTNAPQSALAAAAALHIDVLAGPERAVAATKSYTAE
                     LLSLYLLVDALRGGDGSDAGVLAGAARSLIDDPEPVRELARRFAGVERLVVTGRGYSY
                     PTAREAALKLMETSYVSAQAFSGADLMHGPMAMLQRDSAVIAIVPNGVAGHAIAPVVD
                     KLTELGTNLAIVGAGGSVDHGADAQGSVVRLMLPAGLSEELSPVVEIIPLQLLAHELA
                     VVRGFDPDTPRGLHKVTETW"
     gene            complement(2469..3365)
                     /locus_tag="VGD55_01655"
     CDS             complement(2469..3365)
                     /locus_tag="VGD55_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0869075.1"
                     /translation="MSGVPILGVDAGGSATRAVLVRDGEILARFEEPPLNVLLQSDAH
                     ERLAALIIASGAAAAGLGLAGLRGSTEAVLMEQRLQDVTGVDVVVVDDTEATLVGAFG
                     HGPGIVVIAGTGSNALGRNREGRSARAGGYGFLLGDEGSAYWFGSQAVRAALHSSDGT
                     GPKTDALEAVVAAEYGLDFEGIVRLVYNDPADRQLLARLARTVMALDYDPVMTGILDA
                     GTDCLVGLAQVLGTQLGSGQRAGELPIAMHGGVFENAYVRRRFVEATGAIAAARAPEF
                     GAVDLALQHVQQRASRGTSRSS"
     gene            complement(3378..>4152)
                     /locus_tag="VGD55_01660"
     CDS             complement(3378..>4152)
                     /locus_tag="VGD55_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0869076.1"
                     /translation="VVMAFPIYWAIATSFKRGVDMTKVSPQWFPFPGTLLHYRDAWHK
                     AFFLSSLRNSAIIVGLTVIIAIAAAFLAAVAVARTNFRGRRAYIVLIIVIQMIPQTAL
                     IVPLFVVLSRFHEQNQLIGVTFTYLAFILPFAVWTLRGFVANIPKDLEDAAMIDGCSR
                     FSAFMRVTFPLIAPGLVATAIFAFIQAWNEFTFAYVIIQDNSKETATVWLASFITQEQ
                     VDWGGLMAGATMLSIPIVILFLLVQRHFATGLTAGAVKG"
BASE COUNT          724 a         1401 c         1329 g          698 t
ORIGIN      
        1 acgcgaggtt catggtcgaa ccgaacgccg ccaatctcgc tgccttgctc gcggatcccg
       61 tcgcgactgc gcagatcacc atgctcacgc tcgcccccga acttccgggg gcgctcgagg
      121 cgatacgaac actggccgcc atcgggatcg tcgtctcggt cggccactcc gacgcaaccg
      181 gcgcacaagt gcacgaggcc gtgctcgccg gggcccgcat ggtcacgcac atcttcaatg
      241 cgcaacgtgg tttcgaccat cgcgaacccg gcgtttcggg gcaggcactg tacgaagcgg
      301 ccatgacgat cggcctgatc gccgacttcg agcacgtatc gccggaagct tgcgctatca
      361 cgctgaacgc tgcaccaggg cgggtggccc tcgtgaccga cgcggtcgca ccagccggca
      421 tgcctcctgg cgtctacgag ctcggcggcc aacagatcgc gatctcgact gcggcaccgc
      481 tggcccgcaa tctcgacggg accgtcgccg gctcggcgac gttccttgac caagctgtac
      541 gcaacctcgt cggcttcggg cgcgacatca ccgaagtgct ctgcgccgcc accgtggtgc
      601 cagcagatgc gctgcatcga cccgacctcg gccggctcgc acccggtaca cgcgccgaca
      661 tcgtctggtg gagcgacgac ttggaaccac ttggtacctg ggtcgacggc gtgcaggtgt
      721 acgccgcgaa cgccgccacc gcgagcctct agggttactc catggtgtcg ggaactcagc
      781 tggccgcgtt cgcagcaacc tcgttcgtgc tgatcgtgat ccctgggccg agcgtgctgt
      841 tcgtcatcgg gcgggcactg tcacttggtc gaggtcctgc gatcgccagc gtggtaggaa
      901 acgggcttgg cgtgtacgtg gtggccgtcc tggtggcttt cggattaggc agccttgttc
      961 agcggtccga actcgcgttt gtcgccgtca agattgccgg cgccgcctac cttgtgtggc
     1021 tgggcgtgca agccatccgt catcgccgca gcctggccgc tgctctcggt gcgaccgcga
     1081 ccccgatgtc gcgctggcgg gctgtacggc aagggttcct ggtgggattc gccaacccga
     1141 aagcgctgat cattttcggc gcggtgctgc cccagttcgt cgatcggaca gccggtcgcg
     1201 ttcctgagca aatgctgctg ctctcggcgg ttgccgtcgg catcgcgctg atctccgaca
     1261 cggtctgggt gctggccgcc agcggcgtcc gtggctggtt cgcgagcaat cctcgacggt
     1321 tggccgctgt gggcggcgct ggcggcgtcg ccatgatcgc cgtcggcgtc agtgtcgcca
     1381 ccaccggtcg ccacgactag cccgctacca ggtctcggtg accttgtgca ggccgcgagg
     1441 ggtgtcggga tcgaagccgc gcacaacggc tagctcatgc gcaagtagct gaagcggaat
     1501 gatctcgacg acgggtgaga gctcttcaga aagaccggcg ggcaacatca atcgcacgac
     1561 actcccctgc gcgtcagcgc cgtggtcgac ggaccctcct gctccgacga tcgccaggtt
     1621 cgttcctagc tcggtgagct tgtcgacgac tggcgcgatc gcgtggcccg ccacaccgtt
     1681 gggcacgatc gcgatgacgg cgctatcgcg ttgcagcatc gccatcggcc catgcatcaa
     1741 gtcggccccg gagaacgcct gcgccgacac gtagctggtc tccatgagct tcaacgcggc
     1801 ttccctcgcc gttgggtagg agtacccgcg cccggtgacg acgaggcgct cgacgcccgc
     1861 gaaccgacgt gccaattccc gtaccggctc ggggtcgtcg atcaacgaac gcgcggcacc
     1921 ggcaagaacc ccagcatcgg atccgtcacc gccgcggaga gcatctacga gcaggtacag
     1981 cgacagcaac tctgccgtgt agctcttcgt cgccgcgact gcgcgctcag gccccgccag
     2041 cacgtcgatg tggagtgcgg ccgccgcagc cagcgccgac tgcggtgcgt tcgtaaccgc
     2101 caaagtcagc gcgccgcacg cgcgggcggt tgtcagtgat tgcacgaggt cgggtgagcc
     2161 acccgactgg ctcacggcga tgaacagcac gtcgtgcaga tctggtcgcg cgccgtacgc
     2221 cgtcatcgtc gacggagagg cgagcccggc cggcaagccg aggacgatct cgaccagata
     2281 cttcgtgtag agggccgcat ggtcactcgt accgcgcgcc gcgagcagga cgaagcggat
     2341 tcccgcggcg gagatgtcac tcgcgacgcg cgcaatgtcg gcgcgcccgt cggcgaggat
     2401 tcggttgaac atcgcaggct gttcggcgat ctcgcgcgcc atcagctcgc cgggcccggt
     2461 gtgttgcatc aagaactcct cgaggtgccg cgactcgcgc gttgttgaac atgttggagc
     2521 gctaggtcga cagcgccgaa ctcaggtgcg cgggccgcgg ctatagctcc tgttgcctcg
     2581 acgaagcgcc ggcgcacata cgcattctcg aacaccccac cgtgcatcgc gatcggcaac
     2641 tcgcccgcac gctgcccgct tcctagctgg gtgccgagca cctgcgcgag cccgaccagg
     2701 cagtcagttc cggcatcgag gatcccagtc atcaccgggt cgtaatccaa cgccatcacg
     2761 gtgcgagcga gccgggcaag cagctgccga tccgcgggat cgttgtagac gagccgcacg
     2821 atcccctcga agtcaagccc gtattcggcg gcgactactg cttcgagcgc gtcggttttg
     2881 ggcccagtgc catcgctgga atgcagcgct gcgcgcaccg cctgactgcc gaaccagtag
     2941 gcgcttccct cgtcgccgag caagaagccg taaccgccgg cccttgcaga ccggccttcg
     3001 cgattacggc caagagcgtt cgagcccgta cccgcgatca ccacgatgcc gggcccgtgg
     3061 ccgaacgcgc cgacaagagt ggcctccgtg tcatcgacga cgacgacgtc cactcctgtc
     3121 acgtcttgaa gcctttgctc catcagcaca gcttcagtgc tgccgcgcag accggcgagc
     3181 ccgagtccgg cggcggccgc gccagacgcg atgatcaggg cggccagccg ctcgtgcgca
     3241 tcgctttgga gcagcacgtt cagcggtggc tcttcaaacc gggcgaggat ctcaccgtct
     3301 cgcaccagga cggcccgcgt cgcggatccg ccagcgtcga cgccgaggat cggcactcct
     3361 gacacccgtt gctcccgtca gcccttgacc gcacctgcgg tgagcccagt cgcgaaatgt
     3421 cgctgcacca gcaggaacag aatgacgatc gggattgaca gcatcgtcgc accagccatc
     3481 agcccacccc aatcgacctg ctcctgcgtg atgaacgaag ctagccagac cgtcgccgtt
     3541 tccttgctgt tgtcctggat gatcacgtac gcgaacgtga actcgttcca ggcctggatg
     3601 aaggcgaaaa tggccgttgc tacgagcccg ggcgcgatca gtgggaacgt caccctcata
     3661 aacgcgctga accgcgaaca cccgtcgatc atcgccgcgt cctcgagatc cttcgggatg
     3721 ttggccacga agccgcgaag ggtccatacc gcgaacggca ggatgaacgc gaggtaggtg
     3781 aaggtcacac cgatgagctg gttctgctcg tggaaacggc tgaggacgac gaagagcggc
     3841 acgatcaatg cggtctgcgg gatcatctgg atcacgatga tcagcacgat gtaggcccgt
     3901 cgcccgcgaa agttcgtgcg cgcgacggcg actgctgcga ggaacgcggc cgcgatcgca
     3961 atgatgaccg tgagtccgac gatgatcgcg ctgttgcgaa ggctagaaag aaagaacgcc
     4021 ttgtgccacg cgtcccggta gtgcaacaga gtgccaggaa acggaaacca ttgcggcgac
     4081 accttggtca tgtcgacacc gcgcttgaac gaggtcgcaa tcgcccagta gatcgggaac
     4141 gccatcacga ca
//
LOCUS       DASWVF010000097         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_410311, whole genome shotgun sequence.
ACCESSION   DASWVF010000097 DASWVF010000000
VERSION     DASWVF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410311"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1451)
                     /locus_tag="VGD55_01665"
     CDS             complement(189..1451)
                     /locus_tag="VGD55_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869077.1"
                     /translation="MSHFANSPHPYRAGVVAGTTLAALASAVLGGCASPASHSSQAGT
                     EPIASASAAASPGAASSSAAVSSGPASPTAVTSTEPIGDPDAIQKCLPYRVNQVSPEM
                     LVAGYDTTLGYGTRYLDAIGNGPSQPADAQRESTTPVALCVFDGSFDAPNFVATRAYI
                     MVGSSGASLYPSVFNDDTGIPDRPGGTRPELSPSTVATWSVPPGTPMLPSVDCPTTYG
                     ITNPGSPRPVALLPTPPDVTGSFASYTDALHNIVVLGPSGWSCHAIDAADGNVSIMVT
                     PPGVPSDTMSQPFVRDTREGIHAFLASAGTGSVPDLICAVMPDLVAASWNGRPCPQKP
                     AAETVIRDNPVAAEFYDPAGVVGDGAPSGGNYQAVGRMLDDPAHGDQPEGAASVTCTL
                     PPQPVGLLCDDVVVEFAPFGPFYPIPGG"
     gene            complement(1521..>1999)
                     /locus_tag="VGD55_01670"
     CDS             complement(1521..>1999)
                     /locus_tag="VGD55_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869078.1"
                     /translation="AAGVTAIVTGMTIGRLSSARLTVRHGPDPLLLGAFGLTIVGFGV
                     FWISTNPWLAFTGLFISGLGISVQFPLSITRAVGFSDGRPDLATAYSALGGGFAVGVA
                     PFGLGAIADHLGSHTAMLIVPVFALLGIVGVATTRRQSVVRAVPAAAIGGVGGTAA"
BASE COUNT          356 a          597 c          720 g          326 t
ORIGIN      
        1 tgatgtcgtg actctcggtg acctcggtga cagttccgaa gttcatggtg cccaggcaga
       61 gacggctgac ggctaggcgg gtgcggccaa gttgtgtgta gtccatggtt tctagcttgc
      121 cacgcgattg tcgagcgcga attggccagg gtcggctgga gcgttcccga cgtgtgcaag
      181 ctcgctggtc agccgccggg gattgggtag aacgggccga acggcgcgaa ctctacgacg
      241 acgtcgtcgc agagcaagcc gacgggttgc ggtggaagtg tgcaggtcac ggaggcagct
      301 ccttcaggtt ggtccccgtg cgccggatcg tcgagcatgc gtccgaccgc ctggtagttg
      361 ccgcccgagg gcgcgccgtc tccgacgaca ccggccggat cgtaaaactc cgcggcgact
      421 gggttgtcgc ggatgactgt ctcggctgcc ggcttctgcg gacacggtcg tccgttccag
      481 gacgcagcga ccaggtcggg cataacagcg cagatgaggt caggcacgct gccggttccc
      541 gcgctggcga gaaacgcatg gattccctcg cgggtatcgc ggacgaaagg ctggctcatc
      601 gtgtctgacg ggacgccggg tggcgtcacc atgatcgaca cgttgccgtc tgcggcgtcg
      661 atggcgtgac aactccagcc gctcggtccg agcacgacga tgttgtgcag cgcgtcggtg
      721 tagctcgcga agctaccggt gacatcaggt ggtgtcggca acagcgcgac cggccgcggc
      781 gatccggggt tggtgatccc gtacgtggtc gggcaatcga cactcggcag catcggcgtg
      841 ccggggggaa ccgaccacgt cgccaccgtc gacgggctta actcgggtcg cgtgccaccg
      901 ggacgatcag ggatacctgt gtcgtcgttg aagaccgagg ggtagaggga cgcgccgctg
      961 gagccgacca tgatgtacgc gcgcgtagcg acgaagttgg gagcgtcgaa gctgccgtcg
     1021 aacacgcaca gcgctactgg cgtagtgctc tcgcgttgtg catcagcggg ctggcttgga
     1081 ccgtttccga tcgcgtcgag gtaacgagtg ccgtagccga gggtggtgtc gtagccagcc
     1141 acgagcatct ccggtgagac ctgattcacc cggtacggta agcatttctg gatagcgtcc
     1201 gggtcgccga tcggctcggt tgatgtgaca gctgtgggcg acgcgggccc ggacgaaaca
     1261 gcggcggacg atgacgcagc cccgggcgaa gcagcagcgg aagccgacgc gattggttcg
     1321 gtgcccgctt gcgaagagtg actcgccggc gatgcgcagc cgccgaggac agcgcttgcc
     1381 agtgccgcga gggtggtacc ggcaaccacg ccagcccgat agggatgtgg cgagttggcg
     1441 aagtggctca tcgtcgcgct cctccgtgtc ctgtcgcggt tgccaacata gacgctgagg
     1501 tcgacgtgtc ggttgcgcga ttaggcagct gttcccccga cgccaccgat ggcggcggcg
     1561 ggtacggcac gcacaacgga ctgccgtcgc gtggttgcga cgccgacgat tccgagcaga
     1621 gcgaagacgg ggacgatcag catcgccgtg tgtgatccga gatggtcggc gatcgcgcca
     1681 aggccgaacg gtgcgacgcc gacagcgaag ccgccgccga gtgcggagta cgccgtcgcc
     1741 aaatcgggtc ggccgtcgga gaacccgacc gcacgcgtga tcgacagcgg gaactgaacg
     1801 gaaattccga gcccactgat gaacagaccg gtgaaggcaa gccacgggtt tgtcgatatc
     1861 cagaagaccc cgaacccgac gatcgtgagc ccgaaagcgc ccagcagtaa gggatccggg
     1921 ccatggcgca cggtcaaccg ggctgatgag agacggccga tcgtcatgcc agtcacgatc
     1981 gcggtgacgc ccgccgccg
//
LOCUS       DASWVF010000098         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_232002, whole genome shotgun sequence.
ACCESSION   DASWVF010000098 DASWVF010000000
VERSION     DASWVF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232002"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..116
                     /locus_tag="VGD55_01675"
     CDS             15..116
                     /locus_tag="VGD55_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HEY0869079.1"
                     /translation="MAAFLNAAFGFCLGCEVYLLSRRVLTTRKGVPA"
     gene            113..952
                     /locus_tag="VGD55_01680"
     CDS             113..952
                     /locus_tag="VGD55_01680"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0869080.1"
                     /translation="MSREAVLVDADWVEAHLSDPNIVLVEVDEDVSAYDKGHIRGAVK
                     IDWKADLQDPVRRDFVDKAGFEKLLSSRGIGNDDTVILYGGNNNWFAAYAYWYFKLYG
                     HENIKLLDGGRKKWELDSRELTDEAVSRPATSYKAKDVDTSIRAFRDDAIAAIGNLNL
                     VDVRSPDEFSGKLLAPAHLPQEQSQRGGHIPTAKNIPWSKAANDDGTFRSDDELRTLY
                     GDAGVDFGKDTIAYCRIGERSAHTWFVLHEILDQANVKNYDGSWTEYGSLVGVPISLG
                     ADG"
     gene            952..1239
                     /locus_tag="VGD55_01685"
     CDS             952..1239
                     /locus_tag="VGD55_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HEY0869081.1"
                     /translation="MCGAVAGGLPAGVNAGKEAVIQGVVRRDGVPVVGYVRLLDGTGE
                     FTAEVPTSATGEFRFFAAPGTWTVRALVPGGSADRTVSAEVGSVSEVDVAV"
     gene            1334..1462
                     /locus_tag="VGD55_01690"
     CDS             1334..1462
                     /locus_tag="VGD55_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869082.1"
                     /translation="MTEILTVLPPALAALAIVVTAGLCVRRLVRGTLSNGGEAAGD"
BASE COUNT          253 a          485 c          497 g          289 t
ORIGIN      
        1 cagcgttcgc gttcatcgct gcatttctca acgcagcctt cggcttctgc cttggctgcg
       61 aggtctacct gttaagccgt cgtgttctca ctactcgcaa gggagttcca gcatgagtcg
      121 cgaagccgtc ctcgtggacg ccgattgggt cgaggcccac ctgtccgacc ccaacatcgt
      181 gctcgtcgag gtcgacgagg acgtttctgc ctacgacaag ggccacattc gtggggccgt
      241 gaagattgac tggaaggccg acctgcaaga ccctgtccgc cgcgacttcg tcgacaaggc
      301 gggctttgag aagttgctgt cgtcgcgcgg catcggcaac gacgacaccg tgatcctgta
      361 cggaggcaac aacaactggt ttgcggccta cgcctactgg tatttcaagc tgtacgggca
      421 cgagaacatc aagctgctcg acggtggccg caagaagtgg gagctcgaca gtcgcgagct
      481 caccgacgag gcagtgtccc ggcccgcgac ctcgtacaag gccaaggacg tcgacacgtc
      541 gatccgggcc ttccgtgacg acgcgatcgc cgcgatcggc aacctcaacc tggtcgacgt
      601 ccgttcgcct gatgagttct cgggcaagct gcttgcaccg gctcaccttc cgcaggagca
      661 gtcgcagcgc ggcggtcaca tccccacagc caagaacatc ccgtggagca aggccgcgaa
      721 cgacgacggc actttcaggt cggacgacga gctgcgcacg ctctacggcg acgccggcgt
      781 cgatttcggc aaggacacga tcgcgtactg ccgtatcggc gagcgttctg cgcacacctg
      841 gttcgtgctg cacgagattc tcgaccaggc caacgtgaag aactacgacg gatcctggac
      901 cgagtacggc tcgctcgtcg gcgtcccgat ctcgcttggc gctgatggct gatgtgtgga
      961 gctgtggcag gcgggctgcc cgcgggcgtc aacgcgggta aggaagccgt cattcagggc
     1021 gtcgttcgac gcgacggcgt gccggtcgtc ggctacgtcc ggctgctcga cggcactggt
     1081 gagttcacag ccgaggtgcc gacgtcggca acgggggagt tccggttctt cgccgcgcct
     1141 ggcacctgga ccgtgcgcgc cctcgtgcct ggcggctcag ccgatcgcac ggtaagtgcc
     1201 gaggtcggtt cggtgtccga ggtcgacgta gctgtctgag gtttcacact tcgcatccct
     1261 ggtggttgtg atggtcgccc aaatcgaccg ttttcaccgc cgccagggat gcgaagtcgt
     1321 ttagggtcgc cgcatgaccg agatcctgac cgtgctgccg ccggcacttg ctgcgttggc
     1381 gatcgtggtg accgctggtc tgtgcgtgcg ccgcctggtt cggggcaccc tctccaacgg
     1441 cggggaagcc gcaggtgact gaggacgatc cgctcggcgg tctcgcagcc gcgttcgaaa
     1501 gtcctttcat cccggacgcc gcgg
//
LOCUS       DASWVF010000099         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_384859, whole genome shotgun sequence.
ACCESSION   DASWVF010000099 DASWVF010000000
VERSION     DASWVF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384859"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGD55_01695"
     CDS             <1..791
                     /locus_tag="VGD55_01695"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY0869083.1"
                     /translation="VDSDASARRIRDGVRSLTDVNVGVVISDTFGRPWRVGVTDAAVG
                     FAGIQAFEDLRGSVDPYGNDLRVTLTCTVDELAAAADLVKGKLNGVPVAIVRGLAVVL
                     HAESLDTQGISQVVRPIDEDLFSLGTRDVLRARVDAVEFSGEPVDAGRLTRALQTAAL
                     AAHPSGWRGLRFTVLPASDFTSTPAFADADVLATAATVIVPHFDEELTTRDLLAAGAA
                     VENVLVAIAIEQLGSCWIWPTSTAADGLLDYPLGIVAVGKPALG"
     gene            complement(822..>1604)
                     /locus_tag="VGD55_01700"
     CDS             complement(822..>1604)
                     /locus_tag="VGD55_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0869084.1"
                     /translation="RTISIVSFSSDPDAKDNFGPFTPGFREVAFGDADALASAIDANT
                     VALLIEPIQGEAGVVIPPTGYLRAVREICSDRGVLFMADEIQSGLGRTGRTFACDHED
                     VRPDVYILGKALGGGIMPVSAVVSTWDVMGALHPGEHGSTFGGNPLACAVGRAVIGLL
                     NTGEYQTRAAVLGEQLSDRLTGLLGSGVVGFRCRGLWAGVDIDPAIATGREVCEELVR
                     RGVLAKDTHGSTIRLAPPLVISSAEVDFAVDALAGALAELKG"
BASE COUNT          269 a          545 c          518 g          272 t
ORIGIN      
        1 cggtcgattc cgatgcttcc gcccgacgga tccgcgacgg cgttcgctcg ctcactgacg
       61 tgaacgtggg ggtcgtcatc agtgacacgt ttgggcggcc atggcgcgtc ggcgtcacag
      121 atgcggccgt gggattcgcc ggcattcagg cgttcgagga tctacgtggt tccgtcgacc
      181 cgtacggcaa cgacttgcgg gtgacactca cctgcacggt cgacgaactc gccgccgctg
      241 ccgatctcgt caagggcaaa ctgaacggcg tgccggtcgc gattgtgcgt gggctcgccg
      301 tcgtgttgca tgccgaaagc cttgacacgc aagggatttc gcaggtcgtg cgccccatcg
      361 acgaagactt gttctcgctc ggcacgcgcg acgttctgcg cgcccgcgtc gacgccgtgg
      421 aattctccgg cgagccggtt gacgccggga ggctcacacg tgcgctgcaa acggcggcgc
      481 tcgccgcgca cccttcgggt tggcgcggac tgcggttcac cgtgctgcca gcgagcgatt
      541 tcacgtcaac accagcattc gcggacgccg atgtgctcgc gactgccgcg accgtgatcg
      601 tgccgcactt cgatgaagag ctcaccacgc gcgatctgct cgcggccggt gcggccgtcg
      661 agaacgtgct ggttgccatc gccatcgaac agctcggatc ctgttggatc tggcctacgt
      721 cgaccgcagc tgatggctta ctcgactacc cactgggaat cgtcgcggtc ggcaagcccg
      781 ctctcggcta agcctcgagt gcggctaacc cttgagctcg gctagccctt gagctcggcg
      841 agtgcaccgg cgagcgcgtc gacggcgaag tcgacctcgg cggagctgat caccagcggc
      901 ggcgcgagcc gaatcgtcga gccatgcgtg tccttggcga gcactcccct acgcacgagt
      961 tcttcgcaca cctcacggcc ggtcgcgatc gccgggtcaa tgtcgacgcc agcccacagc
     1021 ccgcgacagc ggaacccgac cactccgctg ccaagcaagc cggtcagccg gtcggagagt
     1081 tgctcaccga gcaccgccgc acgcgtctgg tactcaccgg tgttcagcaa gccgatgacg
     1141 gcccggccta ctgcgcacgc gagcgggttg ccgccgaagg tgctgccgtg ctcacccgga
     1201 tgcagcgcgc ccatgacgtc ccacgtcgac acgaccgcgg agaccggcat gatgccgccg
     1261 ccgagtgcct tgccaaggat gtagacatcg ggccggacat cttcgtggtc acacgcgaac
     1321 gtgcgaccgg tgcgaccaag acctgactga atctcatcgg ccatgaacag cacgccacgg
     1381 tcgctgcaga tctctcgaac cgcgcgcaga taaccggtcg gcggaatgac cacgccggct
     1441 tcaccttgga tgggctcgat gagcaacgcg acggtgttgg cgtcgatcgc cgatgcaagc
     1501 gcgtcggcgt ccccgaacgc cacctcgcga aaacctggcg tgaacgggcc gaagttgtcc
     1561 ttcgcgtcgg ggtcgctgga aaagctcacg atgctgatcg tgcg
//
LOCUS       DASWVF010000100         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_384870, whole genome shotgun sequence.
ACCESSION   DASWVF010000100 DASWVF010000000
VERSION     DASWVF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384870"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGD55_01705"
     CDS             <1..640
                     /locus_tag="VGD55_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY0869085.1"
                     /translation="GVNPQGVTVHPFKVPSLNELQHDYLWRYQAQLPTRGSIGIFNRS
                     HYEEVLVVRVHPEVLETQHLPAAAVKADIWKRRYREINDWERYLVDNGVRIVKLFLNV
                     SKDEQRRRFISRIDEPDKNWKFSVADAAERMRWDEYQVAYQEMLGATSTAHAPWFVVP
                     ADHKWFARLTASWIILETLMDIDPKYPKLAPDVRAKMLETKEELEAETPRHA"
     gene            670..1269
                     /locus_tag="VGD55_01710"
     CDS             670..1269
                     /locus_tag="VGD55_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869086.1"
                     /translation="MMFGRWVRAARRVDDLVSPAPELGNEVLIDDHWLIRIAGPLFAL
                     FSLLLIPWIAFIALVLPSRQLSRNYDFAWAGFDGMLLIALASTAYFALRRSRYLSVAA
                     TATGTLLIVDAWFDVLTSRRREVPFALALAVFVEVPLALLCWWLSQQTQAIAEKRISL
                     LLPRTRGRLRRDADKAGALDTGRPARRVAWGRKPDERER"
     gene            complement(1280..1813)
                     /locus_tag="VGD55_01715"
     CDS             complement(1280..1813)
                     /locus_tag="VGD55_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY0869087.1"
                     /translation="MSLERGLPPDPFDVLPVKPWFSLESDDITDGAPLALPFVHDSAG
                     GENLSPQLRWSGFPSETKSFAVTCYDPDAPTQSGFWHWVLVNLPADVTQLPRGAGTPA
                     GLPAGAFHVAGDMGTKAYGGAAPPPGDRVHRYVFAVHALDVDKLDVDDSVTPAIVGFN
                     LAFHTLARAAIRPTYQR"
     gene            complement(1860..>2327)
                     /locus_tag="VGD55_01720"
     CDS             complement(1860..>2327)
                     /locus_tag="VGD55_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY0869088.1"
                     /translation="GHGPGAAWAASVAVGDDVEVFGPRGKVRLADAGWQLFAGDESAI
                     PAIAEMAAALTPRTAGTVLIEVADADDEQPIGAPELIDVHWLHRGATPPGESDLLDGA
                     IAALDIPDADRHAYLFGESRVVRRLRDELGRRGLHAAEVSAKGYWNVGRIATA"
BASE COUNT          395 a          769 c          771 g          392 t
ORIGIN      
        1 cggcgtcaat ccgcaaggcg tgaccgtcca tcctttcaag gtcccgtcgt tgaacgagct
       61 gcaacacgac tacttgtggc gttaccaggc gcagctgccc acgcgcggct cgatcgggat
      121 cttcaaccgc tcgcactatg aggaggttct ggtcgtacgc gtgcatccgg aggtgctcga
      181 gacccagcat ctaccggcgg cagcggtgaa ggccgatatc tggaaacggc gctatcgcga
      241 gatcaacgat tgggagaggt acctcgtcga caacggcgtg cggatcgtga agttgttctt
      301 gaacgtgtcg aaagacgagc agcggcgacg ttttatctcg cgcatcgacg agcccgacaa
      361 gaactggaag ttctcggtcg ccgacgctgc ggagcgcatg cgctgggacg agtatcaggt
      421 cgcctaccaa gagatgctcg gcgccacgag taccgcgcac gcgccctggt ttgtcgtgcc
      481 ggcggatcac aagtggttcg ccaggctcac tgcatcgtgg atcatccttg agacgttgat
      541 ggacatcgat ccgaagtatc cgaagcttgc tcccgacgtc cgcgcgaaga tgctcgagac
      601 caaggaagaa ctcgaggccg agacgccccg gcacgcgtga acacgactgc gcgcgggtag
      661 ccaggtctca tgatgttcgg cagatgggtg cgtgccgcga gacgagtcga cgacctcgtc
      721 tcgccggctc ccgaactcgg caacgaggtg ctgatcgacg accactggct gatccggatc
      781 gccgggccgt tgttcgccct cttctcgttg ttgctgatcc cgtggatcgc gttcatcgca
      841 ctcgtcctgc catcgcgtca gctttcacgc aactacgact tcgcgtgggc aggcttcgac
      901 ggcatgctgc tcattgcgct cgcgagcacc gcatacttcg cgctgcgccg atcccgctat
      961 ctctcggtcg ccgcaacggc caccggcacg ctgctgatcg tcgatgcctg gttcgacgtt
     1021 ctcacgtccc gtcggcgcga ggtacctttc gctcttgcgc tggcagtgtt cgtcgaggtg
     1081 ccgctcgcgt tgctgtgctg gtggttaagc cagcagacgc aggccatcgc agagaagcgg
     1141 atctcgctgc tgttgccgcg tacgagaggc cgcttgcgcc gcgacgccga caaggcgggc
     1201 gccctcgaca ccggacgccc ggcgcgacgc gtcgcgtggg gacgcaagcc cgacgagcga
     1261 gagcgctgag ttagcagcgt cagcgctggt aggtcggccg tatggccgcg cgcgcgagag
     1321 tgtggaacgc gaggttgaac ccgacgatcg ccggggtgac ggagtcgtcg acgtcgagct
     1381 tgtcgacgtc gagggcgtgg accgcgaaca cgtaccgatg cacccgatca ccagggggtg
     1441 gcgcggcacc gccgtacgcc ttggtcccca tgtcacccgc aacgtggaag gcgcccgcgg
     1501 gcagccccgc cggtgtaccc gcaccgcggg gcaactgcgt cacgtcagcc ggcaggttca
     1561 ccagtaccca gtgccagaag ccgctttggg taggagcgtc ggggtcgtag caggtgaccg
     1621 caaacgactt cgtctcgctc ggaaagcccg accagcgaag ctgggggctg aggttctcac
     1681 ccccagcgct gtcgtgcacg aacggcagcg cgagcggcgc accgtcggtg atgtcgtcgc
     1741 tctccagtga gaaccagggc ttcaccggca ggacgtcgaa ggggtcaggt gggagtccgc
     1801 gttcaagtga catagctgca ttgtctgtcg aggtatcgct gaccgcaatg agacggcgct
     1861 caggccgtcg cgatccgccc gacgttccag tagcccttcg ccgacacctc tgcggcgtgc
     1921 aggccgcgcc ggcccagctc gtccctgagc cgccgcacga cgcgactctc gccgaacagg
     1981 tacgcgtgcc ggtcggcgtc cggaatatcg agggccgcga tcgcgccgtc gagcaggtcc
     2041 gactcccccg gcggcgtggc gccacgatgc aaccagtgga cgtcgatcag ctcgggcgca
     2101 ccgatcggct gctcgtcatc ggcgtcggcg acttcgatca ggacggtgcc cgcagtacga
     2161 ggcgtgagcg ccgcggccat ctcggcgatc gccggaatcg ccgactcatc tccagcgaaa
     2221 agctgccagc ccgcgtcggc caggcgaact ttccctcggg ggccgaacac ctcgacgtca
     2281 tcgccgacgg ccaccgatgc agcccacgcc gcgccggggc cgtgacc
//
LOCUS       DASWVF010000101         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_104603, whole genome shotgun sequence.
ACCESSION   DASWVF010000101 DASWVF010000000
VERSION     DASWVF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104603"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VGD55_01725"
     CDS             complement(<1..1292)
                     /locus_tag="VGD55_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEY0869089.1"
                     /translation="MSFADLPDFLDRLEKDGDLVRIKAQVDPHLEVTEIVDRVVRAGG
                     PALLFENVKGSKIPLAMNVFGTKARMAKALGVDDLDEIGTRIADLLRPELPKGFSGLK
                     DAISKLGQLRDVPPKTVRKGDCQDVVVKGDDVDLMSLPALHTWPDDGGAYFNLGLTHT
                     KHPETGQRNLGLYRLQRHDKNTIAMHWQIHKDSNAHHAVAERRGERLPVAIAFGCDPV
                     LTYAASAPLPSDIDEYLFAGFLRQDRVDMVECVSVPLQVPADAQVVLEGWLEPGERRP
                     EGPFGDHTGFYTPVEPFPALHVETMTMQKKPVFQSIVVGRPPAEDGPLGKATERIFLP
                     LVKLLIPDIVDMDMPVEGVFHNCVIVSIDKRFPKHAHKVMHAIWGAGMLSLAKLIVVV
                     DADCDVHDYSQVAWRAFGNVDYSHDLAQSVGPVDHPDHA"
     gene            complement(1373..>1595)
                     /locus_tag="VGD55_01730"
     CDS             complement(1373..>1595)
                     /locus_tag="VGD55_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869090.1"
                     /translation="RDGLPGCAVRAAAHRRLRGAWSSFPTGPDADIWWNDTGCQNADN
                     GHVEVYFYGVGLPGGDFPGGVENVFPAAP"
BASE COUNT          268 a          504 c          535 g          288 t
ORIGIN      
        1 gcgtggtcgg gatggtcgac cgggccgacc gactgcgcga ggtcgtggct gtagtcgacg
       61 ttgccgaatg cacgccaggc gacttgggag tagtcgtgca cgtcacagtc ggcgtcgacg
      121 acgacgatga gcttcgcgag cgacaacatg cccgcacccc agatcgcatg catgaccttg
      181 tgcgcgtgtt tgggaaaccg cttgtcgatg ctgacgatca cgcagttgtg aaagacgccc
      241 tcgaccggca tgtccatgtc gacgatgtcg gggatcagca gcttcaccaa gggcaggaag
      301 atccgctcgg tcgccttgcc aagcggaccg tcctcggccg gcggccgacc gaccacgatg
      361 ctctggaaca ccggcttctt ctgcattgtc atcgtctcga cgtgcagcgc ggggaaaggc
      421 tcgaccgggg tgtagaagcc ggtgtggtcg ccgaacggac cctcgggcct gcgctcaccc
      481 ggctcgagcc aaccttcgag gacgacctgt gcgtcggccg gcacctgaag cggcacactg
      541 acgcactcga ccatgtcgac gcgatcttgg cgaaggaacc cggcgaacag gtattcgtcg
      601 atgtcgctgg gcagcggcgc tgaggccgcg taggtgagca ccgggtcgca gccaaacgcg
      661 atcgccacgg gcaaccgctc gccgcgacgt tcggcaaccg cgtgatgggc gttggagtcc
      721 ttgtggatct gccaatgcat cgcgatcgtg ttcttgtcgt ggcgctgcaa gcgatacagg
      781 ccgagattgc gttggccggt ctcaggatgc ttcgtgtgcg tcagccccaa gttgaaatac
      841 gcaccgccgt cgtcaggcca cgtgtgcagt gcgggcaatg acatcaggtc gacgtcgtcg
      901 cctttcacca cgacgtcctg gcaatcgccc ttgcgcactg tctttggggg gacgtcgcgc
      961 agctggccaa gcttgctgat cgcgtccttg agtccggaga agcccttcgg gagttcgggc
     1021 ctgagcagat cggcgatgcg ggtgccgatc tcgtcgaggt cgtcgacgcc cagcgccttc
     1081 gccatgcgcg ccttggttcc gaacacgttc atcgcgagcg ggatcttcga acccttgacg
     1141 ttctcgaaga gcagcgcagg gccgccggca cgcaccacgc ggtcgacgat ctcggtgacc
     1201 tcgaggtgcg gatcgacctg tgccttgatg cgcacgaggt cgccgtcctt ctcaaggcga
     1261 tcgaggaagt cgggcaggtc ggcgaagctc atgtgatcaa gtatcgcccg tcgcgccgta
     1321 ttgccgccgt tcagggcggt tcagccggac gccgacggtg tcagggatcc gttcatgggg
     1381 ccgcgggaaa gacgttctcg acgcctccgg ggaaatcgcc gccgggcagg ccgacgccgt
     1441 agaaatagac ctcgacgtgg ccgttgtcgg cgttctggca tcccgtgtcg ttccaccaga
     1501 tgtcagcgtc ggggccggtc gggaacgatg accacgctcc cctcagtcgc cggtgggcag
     1561 cggcccgcac tgcgcatcct ggcagaccat cacgc
//
LOCUS       DASWVF010000102         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_155579, whole genome shotgun sequence.
ACCESSION   DASWVF010000102 DASWVF010000000
VERSION     DASWVF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155579"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..963)
                     /locus_tag="VGD55_01735"
     CDS             complement(37..963)
                     /locus_tag="VGD55_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0869091.1"
                     /translation="MIEVRDLSKRFGSTVAVEHLSFSVRPGVVTGFLGPNGSGKSTTM
                     RMILGLDRPSSGMTTVNGRPYGRIHRPLHEVGALLDAKAMHGGRSAHNHLLCVAQSNG
                     ISRRRVGECLDLVGLNGVARKRARSYSLGMGQRLGIATALLGDPEVLIFDEPINGLDP
                     EGILWIRTLLRSLASQGRTVFVSSHLMSEMALTADHLVVIGRGRLIADSSTTDLIARS
                     SQGYVHVRTPDAPGLATRLRAAGAVVDAEADTMRVTGLESAAIGELAALDHIVLHELT
                     PRAASLEEAFMELTKDSVDYRTDNAVAFEQAS"
     gene            1144..1380
                     /locus_tag="VGD55_01740"
     CDS             1144..1380
                     /locus_tag="VGD55_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869092.1"
                     /translation="MSARPKTDRPLSQPQFVALVVVLALAGVLALITLAGVAGPHGKC
                     PGETQVSRPGASACWSAYKNLPTTGIVGHPAAAG"
     gene            1409..2104
                     /locus_tag="VGD55_01745"
     CDS             1409..2104
                     /locus_tag="VGD55_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEY0869093.1"
                     /translation="MTTVLLVRHGLTALTGPVLAGRTPGVDLDERGRAQAAAVAQRLA
                     GVALQAIVTSPLDRCRQTAEVIAAGRPVETEPRLIECGYGDWTGQEIKKLAKDPLWKV
                     VQAHPSAVTFPGEGGEAMRDAQVRAVDAIRSWNAKLGTEATWLACSHADIIKAIVADA
                     LGMHLDTFQRIVIDPCSVTVITYTSLRPFVVRVNDTGSDLATLLTPPKKSGRKRSTTA
                     GSDAVVGGGAGAN"
     gene            2115..>2299
                     /locus_tag="VGD55_01750"
     CDS             2115..>2299
                     /locus_tag="VGD55_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY0869094.1"
                     /translation="MPRQIFVYDPPERLVIGTVGQPGERTFFLQARGAGQLTSVVLEK
                     VQVASLAERLDQLLDEVL"
BASE COUNT          400 a          757 c          755 g          387 t
ORIGIN      
        1 cgtgaacaga ttcgcgcgga gcgatcgctg tcaccgtcat gacgcctgct cgaacgcaac
       61 agcgttgtca gtgcggtaat caacgctgtc cttggtcagt tccatgaagg cttcctccag
      121 tgacgccgcg cgtggtgtca actcgtgcaa gacgatgtga tcgagcgctg ctaactcgcc
      181 gattgctgcg gattcgagac cagtcacccg catggtgtcg gcctcggcat cgacgaccgc
      241 acccgctgcg cgcaaccgcg tagcgagccc cggggcgtcg ggtgtccgaa catgcacgta
      301 accctgcgag ctgcgtgcga tgaggtcggt tgtgctcgag tcggcgatca agcggccccg
      361 gccgatcacg acaagatggt cggcggtcaa cgccatctca ctcatcaagt ggctcgagac
      421 gaacacagtg cgaccttgcg aggcgaggct gcgcagcaac gtgcgaatcc acagaatgcc
      481 ttcgggatca aggccattga tgggctcgtc gaagatcagc acctcagggt caccgagcaa
      541 tgcggtcgcg atgccgagcc gctggcccat gccgagggag tacgaacgcg cgcgcttcct
      601 ggcgacgccg ttcaggccaa ccaggtcgag acattcgccg acgcggcgac gcgagattcc
      661 attgctctga gccacacaca gcaggtggtt gtgcgcactg cgaccgccat gcatcgcctt
      721 cgcatcgagc agggcgccga cttcgtgaag gggccggtga atccgcccat agggtcggcc
      781 gttcacagtc gtcatgccac tcgacgggcg atcgaggccc aagatcatgc gcattgtggt
      841 cgacttgccc gacccgttcg ggccgaggaa tccggtcacg acgccgggtc gcacagagaa
      901 cgacaggtgc tcgaccgcca ccgtcgaacc gaacctcttg ctgagatcgc gtacctcgat
      961 caccgttgca tccttccgaa ccactagccc ggctctcacc atgcaggcca gcgcggtagc
     1021 gcggctatcg gggagatccc tggccagacc ctgacccgac ccggtggtgc cacgtctggt
     1081 tgcgggcgcc gcgtcgatac actcgggcct cgacggttga tgaacttgag tgcaggaggc
     1141 accgtgtccg cgaggccgaa gaccgaccgg ccgctttcgc agccgcagtt cgtggccctc
     1201 gtggtggtcc ttgcgcttgc gggagtgctc gccctcatca ctcttgccgg tgttgccggg
     1261 ccgcacggga agtgccctgg cgagacccag gtgtcacggc caggggcgtc ggcctgctgg
     1321 tcggcataca agaacttgcc gacgacgggc atcgtcggcc atccggccgc agccggctga
     1381 gccagcacct gcaccgtagg gtctgcgtgt gaccacggtt ttgctcgtcc gccacggcct
     1441 gaccgcgctg accggtccgg tgctcgccgg tcggacgccc ggcgtcgatc tcgacgagcg
     1501 gggccgagca caagccgccg ccgtcgcgca gcggttagcc ggggtcgcgc tccaggcgat
     1561 cgtgacgagc ccactcgatc gttgccggca gacggccgag gtgatcgccg caggtcgacc
     1621 ggtggaaaca gagccgcggc tcattgaatg cgggtacggc gactggaccg gtcaggagat
     1681 caagaagctc gccaaggatc cgctatggaa ggtcgtgcag gcacatccga gtgcggtgac
     1741 gtttccgggc gagggcggcg aggcgatgcg cgacgcacag gtgcgagctg tcgacgcgat
     1801 ccgttcgtgg aacgcgaaac tcggaaccga ggccacctgg ctcgcgtgct cgcacgccga
     1861 catcatcaaa gccatcgtcg ctgatgcgct gggcatgcac ctcgatacgt tccaacgcat
     1921 cgtgatcgat ccgtgttcgg tcaccgtgat cacgtacaca tcgcttcggc cttttgtcgt
     1981 tcgcgtcaac gacaccggga gcgatctcgc gaccttgctc acccccccga aaaagagcgg
     2041 gcgcaagcgc agtacgacgg ccggcagcga tgccgtggtc ggcggcggcg ccggggccaa
     2101 ctagggtcgt ccatatgccc cgtcagattt tcgtctacga cccgcccgag cgcctcgtca
     2161 tcggcacagt cggccagccc ggtgaacgca ccttcttttt gcaggcgcgc ggtgccgggc
     2221 agctgacaag cgtcgtgctc gagaaggtgc aggtcgcgtc tctcgccgag cggctcgacc
     2281 agctgctcga cgaggtgct
//
LOCUS       DASWVF010000103         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_104718, whole genome shotgun sequence.
ACCESSION   DASWVF010000103 DASWVF010000000
VERSION     DASWVF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104718"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..881
                     /locus_tag="VGD55_01755"
     CDS             105..881
                     /locus_tag="VGD55_01755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0869095.1"
                     /translation="MEPDPPLSVLGSTAVSVAACRAVESVRIDAWFHDELACHVVTTA
                     GFPPTLSRGLVAWVAVRTRFLDELVKTTVMDAVVQVVVVGAGLDSRAYRLDLPRDVTV
                     FEVDHSDVLSMKQRLLDDIELFSSCRRLVVIADLVDDDWLALLAVTGWDPTRATLWIV
                     EGLLVYLTHEERTRLLKRLAAASGPASVLGATLSTRTDNLAHPLWHPADAAEPVEWLL
                     ECGWRAGVQTMAEASEEFGRPVSPELRTSTKGRLVRATLA"
     gene            complement(954..2213)
                     /locus_tag="VGD55_01760"
     CDS             complement(954..2213)
                     /locus_tag="VGD55_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0869096.1"
                     /translation="MRDPRMNWARNITFTAPDLHRPSSVDELRRLVASTRKIRALGTG
                     HSFNTIADSPGDQVSLADLPSVLHIDAERSTVTVGSGVRYGELAPYLQSAGYAVHNLG
                     SLPHICIAGACATGTHGSGVTNGNLATAVRAIEMVTASGDLVALDRDHADFPGAVVAL
                     GMLGIVTSMTLAIEPTYDVAQSVYDELPFTELQDHLDDVLSAAYSASVFTDWRGPLLN
                     AVWLKHRIGSSGVQPAPQQQWFGATLADGPRHPVPGMAVANCTQQLGVPGPWHERLPH
                     FRLDFTPSNGEELQSEYFVARASARGALDALNSIKDRIAPVRQIAEIRTIAADELWLS
                     ECYGRDSLAPHFTWVKDSGAVTPVLAAIEEQLAPFGARPHWGKVFTTSPDLVRSCYDR
                     LPDFTALAARYDPTGKFRNDFVDRYIF"
     gene            2263..>2482
                     /locus_tag="VGD55_01765"
     CDS             2263..>2482
                     /locus_tag="VGD55_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0869097.1"
                     /translation="MEYRQLGSSGLRVSAITLGTMTFGGRGWAAAVGEVDVEGARRQI
                     TMALDAGVNLIDTADVYSDGLSEEIVAKA"
BASE COUNT          388 a          764 c          889 g          441 t
ORIGIN      
        1 gggatacctg ctcgcgcgcc cgttgcgccc ggccgcgttc gcgcaactcg tgctcgcgca
       61 cgactgaggc agcgagcgtc ggcgacccca cgtagcatcg catcgtggag cccgacccgc
      121 cgctgtccgt cctcggatcg actgcggtct cggtcgcggc ctgccgcgcg gtcgaaagcg
      181 tgcggatcga cgcgtggttc catgacgagc tcgcgtgcca cgtcgtcacg accgcgggct
      241 ttccacccac gctctcgcgc ggactcgtgg cgtgggtggc cgtccgtacg cggtttctcg
      301 acgagcttgt gaaaacgact gtgatggacg ccgttgtgca ggtcgtcgtt gtcggtgccg
      361 gactcgactc gcgggcatac cgccttgact tgccgcgcga tgtcacggtg ttcgaggtcg
      421 atcacagtga cgtgctttcg atgaagcagc ggctgctcga cgacatcgaa ctgttcagca
      481 gttgccggcg tcttgtcgtg atcgccgacc tcgtcgacga tgactggctg gcgttgctcg
      541 ccgtcaccgg ctgggatccg acgcgcgcca cgttgtggat cgtcgaaggc ctcttggtct
      601 accttaccca cgaggaacga acccggctgc tgaaacggct cgccgcggcg tccggtcctg
      661 caagtgttct cggcgcaacg ctgagtacgc ggaccgacaa tctcgcgcat ccgctgtggc
      721 atccggctga tgcggccgag cccgtcgagt ggctgctgga atgcggctgg cgggcgggcg
      781 tgcagacgat ggccgaagcg agtgaggagt tcgggcggcc ggtctcgccg gagttgcgga
      841 ccagcacgaa gggtcggctc gtgcgagcga cactggccta gaagatgccg tgcttgctag
      901 aagatgcagt gcttgctgga aaatgcggtg cctgctagaa gatgcggtgc ttgctagaag
      961 atgtaccggt cgacgaagtc gttgcggaac ttgccggtcg ggtcgtagcg ggccgcgagt
     1021 gcggtgaagt cgggcagccg gtcgtagcag ctgcgcacga ggtcggggct ggtggtgaag
     1081 accttgcccc agtgtggccg cgcgccgaac ggcgccaact gctcctcgat cgcggcgagc
     1141 acgggtgtga ccgcgcccga gtccttgacc caggtgaagt gcggcgcgag gctgtcgcgg
     1201 ccgtaacact cgctgagcca caactcatcg gccgcgatcg tgcggatctc ggctatctgg
     1261 cgcaccggcg cgatgcgatc cttgatgctg ttgagcgcgt cgagggcgcc gcgtgccgag
     1321 gcacgtgcga cgaagtattc cgactgcaac tcctcgccgt tgctcggcgt gaaatcgagc
     1381 cggaaatgtg gcagtcgctc gtgccaaggg ccaggcacac cgagttgctg tgtgcagttg
     1441 gcgacggcca tgccgggcac cgggtggcgc ggtccgtcgg cgagtgtcgc gccgaaccac
     1501 tgctgctggg gcgcgggctg gacgcctgat gatccgatgc gatgcttgag ccacaccgcg
     1561 ttcagcagcg gaccacgcca atcggtgaac acgctcgcgc tgtacgcagc cgacagcacg
     1621 tcgtcgagat ggtcttgcag ctcggtgaag ggcagctcgt cgtagacgga ttgcgcgacg
     1681 tcgtaggtcg gctcgatggc cagcgtcatg ctcgtcacga tgccgagcat gccgagcgcc
     1741 acgaccgcac cagggaagtc ggcgtggtcg cggtcgagtg cgaccaggtc accgctcgcg
     1801 gtcaccatct cgatcgcgcg cactgccgtg gcgaggttgc cgtttgtcac gccggatccg
     1861 tgggtgccgg tcgcgcacgc gcccgctatg cagatgtgcg gaagcgatcc aaggttgtgc
     1921 accgcgtagc ccgcgctctg taggtaggga gcaagctctc cataccggac gccggacccg
     1981 accgtcacgg tcgatcgctc ggcatcgatg tgcagcacgg agggcaggtc ggcgagtgag
     2041 acctggtcgc cgggggagtc ggcgatcgtg ttgaaggagt gacccgtgcc gagcgcccgt
     2101 atctttcggg tgctcgcgac cagccggcgc aactcgtcga ccgagctcgg gcggtgcagg
     2161 tctggcgccg taaaggtgat attccgggcc cagttcatgc gcggatcgcg catcgtgctc
     2221 ccctcgtgtg gcttcgctga gagtagtggg caagatagga ccatggaata caggcagctc
     2281 gggagctcag gtttgcgcgt gtcggccatc acgctcggca cgatgacgtt cggcggcagg
     2341 ggatgggccg ctgcggtcgg agaggtcgac gtcgaaggcg cgcgccgaca gatcaccatg
     2401 gcactcgacg caggcgtgaa tctgatcgac accgctgacg tctactccga cggtctgtcg
     2461 gaggagatcg tcgcgaaggc gc
//
LOCUS       DASWVF010000104         6694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_283030, whole genome shotgun sequence.
ACCESSION   DASWVF010000104 DASWVF010000000
VERSION     DASWVF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283030"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..729)
                     /locus_tag="VGD55_01770"
     CDS             complement(1..729)
                     /locus_tag="VGD55_01770"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0869098.1"
                     /translation="MAPCLRVHRTDRHRGYGYRRTAAIIRRRRRAAGRAAANHKRVYR
                     LMRENDLLLRRHFADRPGRVHDGKIITLKSDLRWCSDAFEVQPWNGDRVQVAFVLDCC
                     DREAIAFTAVHRFVDGQMIRDMMLAAVERRFGQHARGLPHAIEWLSDNGSVYTAHETV
                     DQAEALGFIVCTTPPYSPESNGMAESFVKGFKRDYVYLADVRSADELIAELPNWFDDY
                     NRIRPHKGLKMLSPMEYREINLAS"
     gene            899..2500
                     /gene="istA"
                     /locus_tag="VGD55_01775"
     CDS             899..2500
                     /gene="istA"
                     /locus_tag="VGD55_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY0869099.1"
                     /translation="MANVLSEEKRQQVLGLGRLGWSLRRIESATGVRRETASGYLRAA
                     GIAVRQRGGAPSSWPPKPATAEEVSTDYSSENSADSNSKPATAAKVSTDLAVGRSPTA
                     SACEPYRELIGEGLARGRNARAIWQDLVDDHGFAARYASVLRFVRRLRGIQVPEARVV
                     ITTAPGEECQVDYGDGPMVRDKATGKYRRTRLFVLTLGYSRKSVRLIVPKSSSRIWAE
                     LHERAFRQLGGAPRLTVLDNLREGVLTPDVYDPTLNPLFRDVLAHYGVVALPCRVRDP
                     DRKGKVESGVGHAQRTPLRGLRFESVEDAQAYLDRWESNWADTRIHGTTKRQVAVMFA
                     EERPALRPLPLEPFRYYQHGERTVHLDGCVEVEAAYYGAPPGWIGRRVRVQWDGERVR
                     LLDPATGQLLREHLRQERGRHRIHPDDAPSRTPATTVQLLHRAHHAGHHIGTLCDQIH
                     RTDGEIGVKRILGVLSLARKHGAPTVDDAAKAAIELGAPSYRFLRRYLERRPAAPLSL
                     RQVDPLIRQLSLYRDLITQKTGDPT"
     gene            2497..3258
                     /gene="istB"
                     /locus_tag="VGD55_01780"
     CDS             2497..3258
                     /gene="istB"
                     /locus_tag="VGD55_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY0869100.1"
                     /translation="MNLVELDHALRKLRLSGMADVLQTRLHQAQTEKTAAIDLVSALV
                     GDELTRRQDRLFGRRLKQAAFRDADRSLDSFDFAFNKKMNRVLVYDLATARFVAQRED
                     ALFLGPPGTGKSHLAQAIGRAAIQQGHRVIYREAHVLIEQIADASIDGTRKQLLADLA
                     TVPLLIVDDLGMRKLPHTAAEDLLELIMRRYERASTILTSNRPVEDWGKLLGDTAAVT
                     ALLDRLLHHAHVLKCGPRSWRTKIHTELRPEEATS"
     gene            3407..3715
                     /locus_tag="VGD55_01785"
     CDS             3407..3715
                     /locus_tag="VGD55_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869101.1"
                     /translation="MASCLIKQKEPLLKDQGALWADGIVQRSHGGIEDFRSVAAAVDA
                     DVAQKPMPQARRESDPMHAAPVPLPFCAEIIDDPPVRAAIDKAIKKLTPAYRGARSKR
                     "
     gene            complement(3760..4380)
                     /locus_tag="VGD55_01790"
     CDS             complement(3760..4380)
                     /locus_tag="VGD55_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869102.1"
                     /translation="MTAYVAGYSAQAEADERRAAADQWKDWWGKTGRTLPWNFQSAGA
                     TFGVAASAAPNNRRSHVIGGIAYRQQPLGPVEVRVLSSALNEWLRARNAGTGGLQGTH
                     RIADHTVVYPSDGTMIEADGDIVRLLTSALLSSAKEAASTPGDSTAVMLILATMAKMP
                     DRRFVPALETLCATISKLPAWHRVDRFAAGLLEVVEPSRTATGDEG"
     gene            complement(4946..5647)
                     /locus_tag="VGD55_01795"
     CDS             complement(4946..5647)
                     /locus_tag="VGD55_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869103.1"
                     /translation="MSAEYSIGVTPTAALLGEPLIAELRCVVHSDVRETLTFDHRSLV
                     VELDREGLAEPALAFPNRHAVERGGQLLRISSAGGIEDLAAGEERTRTIDLAPLFPEL
                     VLSVGRFAVTYRLEEADPVVRPAPVAVEVASGPEAVSHLIGCLAAESSDLRSRAADLL
                     AVMTAQDFGYETGAAGVSRAEAIRRWQTWWQNVGSALPWNFESNGATFGVVTPQAPSG
                     RSYLLGGVAYPGAHG"
     gene            complement(5648..>6694)
                     /locus_tag="VGD55_01800"
     CDS             complement(5648..>6694)
                     /locus_tag="VGD55_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869104.1"
                     /translation="AKVLRDPANPEAGDLGHDDSPKFEVWKRLEYRNLYRMHTGSNLG
                     FDLASHSTVPNIQPAFTPTFTEYSVGSPTAIPYREYITPLVAPTAAQLPLSGTVQIRS
                     DGPDSRVVVVGGLVVAADGSTAPGTEALTLLGTNVVTGTKRFQKVVSVTVPPSQGRSL
                     FVETSVGLPICAVRPHQAAAAPSFLFDTFAAVQSKAQSWYDANDTQLGVDLAALASSV
                     GAPGYCMVGAAYYHPKRDGRPTTGRTSYYAGYPAVRLTYYGTAFHPDAKWGSVDGVNQ
                     DKMSCLFLNVSGGSYATMVARHEIGHASDHVSYGPGDHCPQRSCLMYAFSANNQFCTK
                     TKEHSVRRTMGWLP"
BASE COUNT         1223 a         2251 c         2145 g         1075 t
ORIGIN      
        1 tcagctcgcc aggttgattt ctctgtactc catgggtgac agcatcttca agcccttgtg
       61 tgggcggatt cggttgtagt cgtcgaacca gttgggcagc tcggcgatca gctcgtcggc
      121 gctgcgaacg tcggcgagat acacgtagtc gcgtttgaag cccttcacga acgactcggc
      181 cattccgttg ctctcgggcg agtacggggg cgtggtgcag acgatgaaac cgagcgcctc
      241 ggcctggtcg acggtctcgt gcgccgtgta cacggagccg ttgtccgaaa gccactcgat
      301 ggcgtgcggc aggcctctgg catgctggcc gaatcgccgc tcaacggcgg ccagcatcat
      361 gtcgcggatc atctgcccgt cgacgaaccg gtggacggcc gtgaacgcga tggcctcgcg
      421 gtcgcagcaa tccagcacga acgccacctg cacgcggtcg ccgttccagg gctgcacctc
      481 gaaggcgtcc gaacaccacc gcaggtccga tttcagcgtg atgatcttgc catcgtgaac
      541 gcgcccaggt cgatcggcga agtgtcgacg gagcaggagg tcgttctcgc gcatgagccg
      601 gtaaacgcgc ttgtggttgg ccgccgctct tcctgccgcc cgcctgcgcc gtcgaatgat
      661 cgcggctgtc cgccggtagc cgtaccctcg atgccgatcc gttcgatgga cccgaaggca
      721 cggagccacg aaacgagtgc gaagtagccg tcaggtgtcg ctgcaaatgt ttcgcagtct
      781 cggcgccggg cctcggtgat cacccaaaac cggccatgaa agatcgcctc aaaaccggcc
      841 accagatgta gcggtcgaaa cgttgacgag gcggagggcc acgtggtcct ccgccgggat
      901 ggccaacgtc ttgagcgaag aaaaacgaca gcaggtactt gggcttgggc gactcggctg
      961 gtcgctgcgg cggattgaga gcgcgacagg cgttcggcgt gagacggcca gcggctacct
     1021 gcgggcggcc ggaattgcgg tgcgccagcg aggcggagcg ccgtcgtcgt ggccgccaaa
     1081 gccggccacc gcggaagagg tgtccaccga ctattcctcg gagaattcgg ccgattcaaa
     1141 ttcaaaaccg gccaccgcgg cgaaggtgtc caccgacctc gcggtcgggc gatcgccgac
     1201 agccagcgcc tgcgagccgt accgcgagct gatcggggag ggcctggcgc gcggccggaa
     1261 cgcacgggcg atctggcagg acctggtcga cgaccacggc ttcgcggctc gctacgccag
     1321 cgtcctgcgc ttcgtccggc gcctgcgcgg catccaggta cccgaagcgc gcgtggtgat
     1381 cacgacggcg ccgggcgagg agtgccaggt cgactacggc gatgggccga tggtgcgaga
     1441 caaggccaca ggcaagtacc gccgcacgcg gctcttcgtg ctgacgctcg ggtacagccg
     1501 caagtcggtg cggctgatcg tgcccaagtc cagctcgcgg atctgggcgg agctgcacga
     1561 gcgcgccttc cgccagctcg gcggagcacc acgcctcacg gtcctcgaca atctgcgcga
     1621 gggcgtgctg acgccggacg tgtacgaccc gacgctcaat ccgctctttc gcgacgtgct
     1681 cgcccactac ggcgtggtgg cgctgccctg ccgcgtccgc gatcccgacc gcaagggcaa
     1741 ggtcgaatct ggcgtcggcc acgcgcagcg gacgccgctg cgtgggctgc gcttcgaatc
     1801 ggtcgaggat gcgcaagcgt acctcgatcg ctgggagagc aattgggccg acacgcgcat
     1861 ccacgggacg acgaagcggc aggtcgccgt gatgtttgcc gaggagcgcc ccgcgctccg
     1921 gccgctgcca ctcgagccct ttcgctacta ccagcacggc gagcgaaccg tgcacctcga
     1981 cggatgcgtc gaggtcgaag ccgcttacta cggcgcgccg ccgggctgga tcggccggcg
     2041 cgtgcgcgtc cagtgggacg gcgagcgcgt gcgtctgctc gacccggcga ccgggcagct
     2101 actgcgcgag catctgcgcc aggagcgcgg gcgtcatcgc atccacccgg acgacgcgcc
     2161 atcgcgcacg ccggcgacga cggtgcagct cctgcaccgc gcccaccacg ccggccacca
     2221 catcggcacc ctgtgcgatc agatccaccg taccgacggt gagatcggcg tgaagcggat
     2281 cctgggcgtg ctgtcgctgg cccgcaaaca cggtgcgccg acggtggacg acgcagcgaa
     2341 ggcggccatc gagctgggcg cgcccagcta ccgcttcttg cgccgctacc tcgaacgccg
     2401 gccggccgcg ccgctgtccc tgcgccaggt cgacccgctg atccgccagc tgtccctgta
     2461 ccgcgacctc attacccaga agacaggaga tccaacatga acctcgtaga gctcgatcac
     2521 gcactccgca agctgcggct gtccggcatg gccgacgtcc tgcaaacgcg gctacaccag
     2581 gcgcagacag agaagaccgc tgccatcgac ctcgtctcag ccctcgtcgg tgacgagctg
     2641 acgcggcgac aagaccggct cttcggccgc cgtctcaagc aagcggcctt ccgcgacgct
     2701 gaccgctcgc tcgacagctt cgatttcgcc ttcaacaaga agatgaaccg cgtcctcgtc
     2761 tacgacctgg ccaccgcccg cttcgtcgcg cagcgcgagg acgcgctctt cctcggaccg
     2821 ccaggcaccg gcaagagcca tctcgcccag gccatcggcc gcgcagcaat ccagcaaggc
     2881 catcgcgtca tctaccgcga agctcacgtg ctcatcgagc agatcgccga cgccagcatc
     2941 gacggcaccc gcaagcagct cctcgccgac ctggccaccg tcccgctgct catcgtcgac
     3001 gacctcggca tgcgcaagct cccgcacact gccgccgagg acctgctcga gctgatcatg
     3061 cgtcgctacg aacgggcgtc gaccatcctc acctccaacc gccccgtcga ggactggggc
     3121 aagctcctcg gcgacaccgc cgccgtcacc gcactcctcg atcgtctcct ccaccacgcc
     3181 cacgtcctca agtgcggccc ccggagctgg cgcaccaaaa tccacacaga attgcgtccc
     3241 gaggaggcta cgagctagaa acctaccagt ctcgattgct ccgctggtgg ccggttttga
     3301 cccgatccct ggtggccggt tttgaggtga tccccgaggg ctgggtctct gcggccgacc
     3361 ggcctatacg cgcagtgacg tccagcgcga cgtcgaggga tacgcggtgg cgagctgtct
     3421 cataaagcaa aaggagcccc ttctcaaaga ccaaggggcg ctctgggcgg acggcatcgt
     3481 gcaacggtcg cacggtggca tcgaagactt tcggagtgtt gcggccgccg tcgacgctga
     3541 cgtcgctcag aagccgatgc cgcaggcacg acgcgagagc gatccaatgc atgcagcgcc
     3601 ggtccctttg ccgttctgcg cggagatcat cgacgacccg ccggttcgcg cggcgattga
     3661 caaagcgatc aagaaactga ctccggccta tcggggggcg agaagtaaac ggtgagctcg
     3721 ccaggtcgtc gaactagctg ggccggggca tcatggtcgt caaccttcat cgccggtggc
     3781 cgtgcgagat ggctcgacca cctcgagaag accggccgca aagcggtcca cacggtgcca
     3841 agcgggaagt ttggagatgg tggcgcatag ggtttcgagc gcgggaacaa accgtcggtc
     3901 cggcatcttc gccatcgtcg caagaatcaa catgacggcg gtggagtctc cgggtgtcga
     3961 ggccgcttct ttagctgatg acagcaaggc cgacgtcagc agacgaacga tgtctccgtc
     4021 cgcttcgatc atggtgccat ctgaaggata gacgacggtg tggtcggcga tccgatgggt
     4081 gccttggagc cctcccgtgc ccgcattccg cgcgcgaagc cattcgttca acgccgacga
     4141 cagtacgcgg acttcgaccg gaccgagtgg ctgctgccga taggcgatgc cgccgataac
     4201 gtgcgagcgc cggttgttcg gagcggcgga ggcagccact ccgaacgtcg ccccggccga
     4261 ctgaaagttc cacggcagcg ttctcccagt cttcccccac caatctttcc actggtcggc
     4321 cgccgctctg cgttcgtcgg cctctgcctg cgctgaatat ccggcgacgt acgcggtcat
     4381 ccggtgaatc agaccggctg cacgttcgcg aacctcggca ttctcgtgcg aaagcagagc
     4441 cagaaggccg gagatggcat cgggacctga ctcgacagtg acgctcgccg gacctacacg
     4501 cctgacccgg cctccatcct cgagctcgta gtgagcccgg aattcgccgg tgtcgagcgc
     4561 caaccgcgga aagacgctga cgaggtcgaa ctctcgatcg aggcgctctc cgggctgtaa
     4621 gtcacggcga accgcgccgc cgagccgcac caaccggttt ccttgctgaa ccgccatgtg
     4681 gttggggaaa gagagctccg gctcggcgcg ggacgaacgg cgtcccatgg ccaataccaa
     4741 cgatgcgtcg gcaaacgtgg tgacgtttgc ggttcgcgag gtccgacagt ccattctgag
     4801 aagaagtcga tcgccgagca gtgcgcgcgc cgggcgaaca tcgacggagt atttcgccac
     4861 cggctctgca accgcgcgcc tggcgatcat cggcgccgcg acgatgaagg acagagcggc
     4921 tcgtctgcct atctgcccgg cagctttacc catgggcgcc gggataggcc acgccgccca
     4981 gcaggtagct gcgacccgaa ggcgcttgag gtgtaacgac tccgaatgtg gcgccgttgg
     5041 actcgaagtt ccatggcagg gccgagccga cgttttgcca ccacgtctgc caccgacgga
     5101 tcgcctcggc ccggctcacc cctgcagcgc cggtctcgta tccgaagtct tgcgccgtca
     5161 tcaccgccaa cagatcggcc gcgcgcgacc ggaggtcgga agactccgcg gccaaacacc
     5221 caatcagatg actgaccgcc tctggtcccg aggcgacctc aacagcgacc ggcgcaggac
     5281 gcacaacagg gtcggcttcc tcgagacgat aggtcacggc gaatcgcccc acactcaaga
     5341 caagctcggg gaatagcgga gcaaggtcga tcgtgcgcgt gcgctcttcg ccagccgcaa
     5401 ggtcctcgat tccgccagcg gatgaaatgc gaagcaactg gccgccgcgc tcaactgcgt
     5461 gtcggttcgg aaatgccaac gctggctcgg ccaacccctc tcgatcgagc tcgacgacca
     5521 gggaacgatg gtcaaaggtc agggtctcgc gcacgtcaga gtgaacgacg cagcgaagct
     5581 ccgcgatgag cggttcgccg agaagcgccg cggtcggcgt gacgccaatc gagtactcgg
     5641 cggacattta cggcaaccat cccatcgtac gccggaccga gtgctctttc gtcttcgtac
     5701 agaactggtt attcgccgag aacgcgtaca tgagacatga cctctgcggg cagtggtccc
     5761 cgggcccata cgagacatgg tcggaggcgt gaccgatttc atgacgcgca accatcgtcg
     5821 cgtacgaccc gccggatacg ttcaggaaca gacaggacat cttgtcttga ttcacgccgt
     5881 caaccgaccc ccacttcgca tcgggatgaa aggcggtccc gtagtacgtg agccgtacgg
     5941 caggataacc ggcgtagtaa ctggtgcgac cggtggtcgg ccgaccgtcc ctcttcgggt
     6001 gatagtacgc ggcgccgacc atgcaatagc ccggcgcgcc caccgaactg gcaagcgcag
     6061 ccaggtcgac gccaagctgc gtatcattcg cgtcgtacca gctctgggct ttcgactgaa
     6121 cagcggcaaa cgtatcgaag agaaagcttg gtgccgcggc tgcctgatga ggccggacgg
     6181 cgcagatggg cagtccgacc gacgtctcga cgaacagcga gcgcccctgc gatggtggca
     6241 ccgtcacact aacgaccttt tgaaaccgct ttgtccccgt cacgacgttg gtccccaata
     6301 gcgtgagcgc ttccgtcccg ggggcagtcg agccatccgc cgctacgacc agcccgccca
     6361 cgacaaccac tcgcgaatcc ggtccgtcgc tgcggatctg aaccgtcccg ctcagcggta
     6421 gctgtgccgc cgtcggggca acaaggggcg tgatgtattc gcgatacgga atcgcggtcg
     6481 gtgagcccac cgagtactcg gtgaacgtcg gcgtgaaggc gggctggata ttcggcacgg
     6541 tgctgtgtga cgcgaggtcg aagcccaggt tcgacccggt gtgcatccgg taaaggttgc
     6601 gatactcgag acgtttccag acctcgaatt tcggggaatc gtcatggccg agatcgccgg
     6661 cctccgggtt ggcgggatcg cgcaacacct tcgc
//
LOCUS       DASWVF010000105         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_385056, whole genome shotgun sequence.
ACCESSION   DASWVF010000105 DASWVF010000000
VERSION     DASWVF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385056"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGD55_01805"
     CDS             complement(<1..287)
                     /locus_tag="VGD55_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY0869105.1"
                     /translation="MGGVRVLVGTRKGAFILSADADRRAWQVTGPHFPGWEIYHVAGS
                     PADPSRLYASQSSGWSGQLIQRSDDGGSTWQAMGNNFAYDGDAGTHQWYDGT"
     gene            452..754
                     /locus_tag="VGD55_01810"
     CDS             452..754
                     /locus_tag="VGD55_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869106.1"
                     /translation="MSEAIILEFKGAGVDEYNAVNKILGIDPTTGEGDWPAPLLNHTA
                     GVTADGDLVVYEIWESRAAQEEFMARLGPALHEVGIAAPVRMEWFEVKGHYHTHSH"
     gene            771..1799
                     /locus_tag="VGD55_01815"
     CDS             771..1799
                     /locus_tag="VGD55_01815"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0869107.1"
                     /translation="MPADDIAPLTTHTAFTQWEFAPIVSVGVVVVALAYVLGVVVVRR
                     RHPLGPWPVRWTASFLSGCAVIVIATQSSIGAYDDVLFWIHMVQHVLLLMVAPPLLLL
                     GRPVTLLLHATRNPAHSAVKRFVRSRVVNAVTFPLLGVVLYIATVVGTHLTGFMNLTL
                     QHPVAHDLEHVVYIVVGYLYFLPLIGREPIKWRLSFTTRLFLLVLAMPVDTFTGVVLT
                     QANHELFPAYVGRRNWGPSLVADLHAGGAVMWIGGDFVMLVMLLVLFAGAARSRQSLD
                     GGAWIEAARANSLSDRGALTTGPGSGPSQVDDNDAQLAAYNAYLATLGDEHDVNGTPH
                     ATKSHDAR"
BASE COUNT          309 a          630 c          645 g          366 t
ORIGIN      
        1 gtgccgtcgt accactggtg ggtgccggcg tcgccgtcgt acgcgaagtt gttgcccatc
       61 gcctgccagg tgctgccgcc gtcgtcggag cgctgaatca gctggcccga ccagccgctc
      121 gactgcgagg cgtacagccg agacggatcg gcgggggagc cggccacgtg gtagatctcc
      181 cacccgggga agtgcggtcc ggtgacctgc cacgcccgcc ggtcggcgtc cgcgctcaaa
      241 atgaatgcgc ccttacgcgt gccgacgagc acccgaacgc cacccatgcc aactccttcc
      301 gatgtgcctg atcgcaccct agttgtgagt agggacgatg gccgcctggg gaactcatcg
      361 gtcgtagcac cagtgttgaa ggcaaccgct cccaggcgta gcgtcctcga cgcgcgcagg
      421 aggctcgctt tcgacaaacg gatggagaga tatgtccgag gccatcatct tggagttcaa
      481 gggcgccggg gttgacgagt acaacgcggt gaacaagatc ctcggcattg atcccacgac
      541 gggcgagggc gactggcccg cgccgctgct caaccacacc gctggcgtga ccgctgacgg
      601 tgacctcgtc gtctacgaga tctgggagtc gagggcagct caggaggagt tcatggcgcg
      661 gctggggccg gcactgcacg aggtcgggat cgcagccccg gtgcgcatgg agtggttcga
      721 ggtcaagggg cactatcaca cgcactcgca ctgagtgaaa ccatgcaggc gtgcctgccg
      781 atgacatagc cccgctgacg acgcacaccg cgttcacgca gtgggagttc gcgccgatcg
      841 tcagcgttgg cgtggtcgtg gtggcgctcg cgtacgtgct cggcgtggtc gtggttcgtc
      901 gccggcaccc attgggtccg tggcccgtgc gctggacggc gtcattcctc tccggctgtg
      961 ccgtcatcgt gatcgcgacg caaagcagca tcggtgcgta cgacgacgtg ctgttttgga
     1021 tccacatggt tcagcacgtg ctgctactca tggtcgctcc gccgttgctg cttctaggcc
     1081 ggccggtgac gttgctcctg cacgccaccc gcaatcccgc gcacagtgcg gtcaagcggt
     1141 tcgtgcgctc gcgcgtggtg aacgccgtca catttccgct gctcggcgtc gtcctttata
     1201 tcgcgacggt tgtcggaacc catctcaccg gtttcatgaa cctgacgctg cagcaccccg
     1261 tcgcgcacga cctcgagcac gtggtctaca tcgtcgtcgg ttacctgtac ttcctcccgt
     1321 tgatcggccg cgaaccgatc aagtggcgcc tgtcgttcac gacgcgactc ttcctgctcg
     1381 tgctcgcgat gcccgtcgac acgttcaccg gcgtcgtgct gacgcaggcg aatcacgaac
     1441 tgtttcctgc gtatgtcgga cgccgaaact gggggccgag cttggtcgct gacctccacg
     1501 cgggcggcgc ggtgatgtgg atcggcggcg acttcgtgat gctggtcatg ttgctggtgc
     1561 tctttgcagg tgcggcacgt tcacgtcaat cgctcgacgg cggtgcgtgg atcgaagccg
     1621 cgcgggccaa cagcctgagc gaccgtgggg cactcacaac ggggcccggt tctggcccgt
     1681 cgcaggtcga cgacaacgat gcgcaactcg cggcgtacaa cgcctacctc gccaccctgg
     1741 gcgatgagca cgatgtaaac ggcactccgc acgcgacgaa atcgcacgat gcgcggtaga
     1801 cggtcggtcg tcgctggact tgcggttgct gcgttggtgc tcgtggcagc ggcgatcgga
     1861 cggttctccc cgtcgtcccc gaaaaggccg aacgccaaca tcgggacgac gctcgatcag
     1921 gcgctgccgt cgtgggcgac gtccatggtt
//
LOCUS       DASWVF010000106         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_436132, whole genome shotgun sequence.
ACCESSION   DASWVF010000106 DASWVF010000000
VERSION     DASWVF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436132"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VGD55_01820"
     CDS             complement(<1..1006)
                     /locus_tag="VGD55_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY0869108.1"
                     /translation="MSGPTSRVFVARLAGVVVFDPNGDQVGRVRDVIVLLRPGTGQPR
                     VLGLVVEVPPRRAIFLPITRVTGIEAGAVMTTGTVSLRRFAKRDTETLVLGQLLDRKV
                     TLIESGEGVTVTDVAMERTRARDWVLAKVAVRAEGRRVRRRGAIRVVDWEAVTGLSLE
                     EHAQGAANLLAAFDQLRPADLAGVMHDLSEKRRAEVAAALDDERLADVLEELSEEEQV
                     EILATLEGERAADVLEAMGPDDAADLLAELPEAEKERLLGLMLPEEANPIRRLLDYSW
                     NTAGGLMTTEPVILDPTATVAEALAHVRNPDLSPATASQVYVVRPPTDTPTGRYLGVA
                     H"
     gene            1246..1713
                     /locus_tag="VGD55_01825"
     CDS             1246..1713
                     /locus_tag="VGD55_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869109.1"
                     /translation="MKRASARGFGAQRAREAHIGDYESAMFGVRHAVTDLESARTHGE
                     SPSVLRHAHGRIDEALAKAAAAATEVYTGLFDAAGGMYHAETDPEVALWKRRMNTALT
                     LRSQHQMAQGDEEAALPIAGVQVRTRAAFGPRQAGLDFGVDHDATLRRAGSTT"
     gene            1779..3440
                     /locus_tag="VGD55_01830"
     CDS             1779..3440
                     /locus_tag="VGD55_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0869110.1"
                     /translation="MPALLQAITAIAAQTNTAAVLRTFVVAAAELVHADYGAFAVLDG
                     DGAVVDLVELPSHQFGDDQFSDDVIERLGRLPERSGTLARLIGTGGPIRLDDIASHAA
                     CVDFAPRHPQMAALLGTPTQVGGRVFGVLYLTRTSDRPVFTGDQELEIRALSTQAGLA
                     IANARAIEAGRLREESLRTLGFVATGLSAGLELADVLPTVIRQARDIVDADFAAVALP
                     SDDPNMLTVQYGDGVDAAHLIGSKVPIDTSLTGIVMRSGLMLEAGNLRTASLDAPTRL
                     VSESHEARLLFGPALFVPVGSLPAVHGVLVVFNRSGRPAFDRQAVTTLNAFAAQVAVS
                     LELAEHRTGDTRLALVEDRERIARDLHDVVIQRLFAIGLTLESASRSIAELDAGTQVS
                     RAIDELDETIKEIRTTIFGLQGPVGTDASGQAGLRARMIAVGEQAIPALGFVPSLRFD
                     GLVDTTVPDRLSDQVVAVLREALSNAARHAHARHVDVVLVVTRDTVTLSVDDDGVGII
                     ETGRRSGLRNMEVRAKASGGNLVLADRVPSGTHIEWQVPMRGRAR"
     gene            complement(3469..4182)
                     /locus_tag="VGD55_01835"
     CDS             complement(3469..4182)
                     /locus_tag="VGD55_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869111.1"
                     /translation="MTELSQGTQQIGTQTATQPVGTRQIRVFLLDDHEVVRRGVRDML
                     NTAPDLVVVGEGSTAAEALAAVPTLMPDVAVLDVRLPDGDGVVVCRELRSQLPNLACL
                     MLTSFADDDALFEAIMAGASGYVLKQIRGGDLVNAIRTVASGQSMLDAGTTAQVMERL
                     RTGTRAEDPLSSLTDQERRILDLIGEGLTNRQIGERLFLAEKTVKNYVSSLLGKLGMQ
                     RRTQAAVMVSRVHTRSELR"
     gene            complement(4232..4633)
                     /locus_tag="VGD55_01840"
     CDS             complement(4232..4633)
                     /locus_tag="VGD55_01840"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0869112.1"
                     /translation="MAEVAAVGAIAIRDGALLLIRRGHAPSRGRWSLPGGRVEPGETS
                     QEALVREVAEETGLVVEVGRLVGEVVRPGPGGVTYRIRDFEVTPTGGVPVAGDDATEV
                     AWVAIEHLASYQLSAGLLRTLRAWAVIPRKA"
BASE COUNT          743 a         1604 c         1568 g          786 t
ORIGIN      
        1 gatgcgcaac gccgagatag cggccggtcg gggtgtcggt cggaggccgc acgacgtaga
       61 cctggctggc cgtggcgggg gacaggtcgg gattgcgcac gtgcgcgagc gcctcggcca
      121 cggtcgcggt cgggtcgagg atcaccggct cggtggtcat caggccgccg gcggtgttcc
      181 acgagtagtc gagcaggcgc cggatcgggt tggcctcttc gggcagcatg agtccgagaa
      241 gccgctcttt ctccgcctca ggcagttcgg ccagcaggtc agccgcgtcg tctggaccca
      301 tcgcctcgag cacatcagcc gcgcgctcgc cctcgagggt cgcgaggatc tcgacctgtt
      361 cctcttcgga caattcttcc aggacgtcgg ccagccgctc gtcgtcgagc gccgcggcga
      421 cctcggctcg cctcttttct gacaggtcgt gcatcacacc cgcgaggtcg gccgggcgca
      481 actggtcgaa agctgcgagc aggttcgccg caccttgggc gtgctcctcg agcgatagac
      541 ccgtcaccgc ctcccagtcg acgacccgga tcgcgccgcg gcggcgcacc cggcgtccct
      601 cggctcgtac ggcgaccttc gcgagcaccc aatcgcgggc ccgcgttcgc tccatcgcga
      661 cgtcggtcac cgtcacgccc tcgccggact cgatcaaggt gaccttgcgg tcgagcagct
      721 ggccaagcac cagagtctcg gtatcgcgct tcgcaaagcg ccgcaggctc acggtgcctg
      781 tggtcatcac ggcgcccgcc tcgatgccgg tgaccctcgt gatcggcagg aagatcgccc
      841 ggcgtggggg gacttcgacc acaagcccaa ggacgcgagg ctgtccggta cctggccgga
      901 gcagcacgat gacgtcgcgc acccggccga cctggtcgcc gttggggtcg aacacgacga
      961 cgccggcgag gcgcgcgacg aacaccctcg acgtcggccc gctcatgtgc ggaggttatc
     1021 gccgccaggg cgcacgcagt gcgccttaac gagggacgcg cggacgcagg cgccgaccgc
     1081 tagccaccga tcaggcctgc ccctttgccg aaaccgccct gtcggaacgg ttgaaggtcc
     1141 accgcggggg catcacccta taaggacgac tgagcacaaa tgggcggacg gcgaggagct
     1201 ggcgacggtg aggtcgatga tcggtcgacg acgcgtgagg gtcgtatgaa gagggccagc
     1261 gcccgagggt tcggcgccca gcgggctcga gaggcgcaca tcggcgatta cgagagcgcg
     1321 atgttcggtg tccggcacgc ggtcaccgat cttgaaagcg cgcgaaccca tggcgagtcg
     1381 ccttcggttt tgcgacacgc ccacgggcgc atcgacgagg cgttagccaa ggcggccgcc
     1441 gcagcaaccg aggtttacac ggggctcttc gacgcagcgg gcggcatgta tcacgcggag
     1501 accgatcccg aggtcgcact ctggaagcgc cggatgaaca ccgcgctgac ccttcgctcg
     1561 cagcatcaga tggcgcaggg cgacgaggaa gctgcgttgc ctatcgccgg tgtgcaggtc
     1621 cgcacccgtg ccgccttcgg gccgcggcaa gcgggcctcg acttcggggt cgatcatgat
     1681 gcgacgcttc gtcgcgcggg gtcgacaacc tgagcgcgag cagcgccgcg ttcgcgccgc
     1741 cgtccgtcag cgatgagcgg cgtttccgca gtgaccgggt gcccgctctc ctccaggcca
     1801 tcaccgcgat cgcggcgcaa accaacacgg ctgcggtgtt gcgaacgttc gtcgttgctg
     1861 ccgccgagtt ggtccacgct gactacggcg cgttcgcggt tcttgacggc gacggtgccg
     1921 tcgtggacct cgtcgaactc ccgtcgcacc agttcggtga cgaccaattc tcagacgatg
     1981 tcattgagcg cctcggccgg ttgcccgagc gttccggaac gctcgcgaga ctgatcggca
     2041 ccggcggacc gattcgcctc gacgacatcg cctcgcacgc tgcctgcgtg gactttgctc
     2101 cgcggcatcc ccagatggct gcgctgctcg gaacgcccac ccaggtcgga ggccgcgtct
     2161 ttggcgttct ctacctgacc cgaacttctg accgtccagt gttcacgggt gatcaagagc
     2221 tggaaatccg agccctcagt acgcaggccg ggcttgcgat agcgaacgcc cgcgcgatcg
     2281 aggcgggacg cctccgcgag gaatcgctgc gcacgctcgg tttcgtggcg acgggattgt
     2341 ctgccgggct cgagctcgcg gatgtgttgc caaccgtgat tcggcaggct cgcgacatcg
     2401 tcgacgctga cttcgcggct gttgcacttc cgagcgacga cccgaacatg ctgacggtgc
     2461 aatatggcga cggagtcgac gctgcgcacc tgatcggctc gaaggttccg atcgacacgt
     2521 cgttgaccgg catcgtgatg cgctcggggt tgatgctcga ggcgggcaac ctgcgcaccg
     2581 catcccttga cgcacctact cgtctggtga gcgaaagcca cgaagcgcgg ctgctgttcg
     2641 ggcccgcatt gttcgtgccc gttggatcac ttcccgccgt gcacggcgta cttgttgtgt
     2701 tcaaccgctc gggtcgacct gcattcgacc gacaggcggt cacgacgttg aacgcgttcg
     2761 ccgcgcaggt cgcggtgtcg ctcgaactcg ccgaacaccg cactggcgat acgcgactgg
     2821 cccttgtcga agaccgcgaa cgcatcgccc gcgatctgca cgacgtcgtg atccagcggc
     2881 tgtttgctat cgggttgacg cttgagagcg cgagtcgctc gatcgcggaa ctcgacgcgg
     2941 gcacccaggt ttcccgcgcg atagacgaac tcgacgagac catcaaggag atccgcacca
     3001 cgatcttcgg gcttcaggga cccgttggca ccgacgccag cggccaggcg ggccttcggg
     3061 cgcggatgat cgcggtcggc gagcaggcga tacccgcgct ggggttcgtg ccgtccttgc
     3121 ggttcgacgg cctcgtcgat acgaccgtgc ccgaccggct ctccgaccag gtagtcgcag
     3181 tgctgcgcga agcactgtcg aacgctgcca ggcacgcgca tgctcgccac gtcgatgtgg
     3241 tgctcgtcgt tacccgcgac acggtcacgt tgtcagtcga cgacgacggc gtcggcatta
     3301 tcgagaccgg acgtcgcagc ggtctacgca acatggaagt gcgggcgaaa gccagcggcg
     3361 gcaacctcgt gcttgccgac cgggtgccgt caggcactca catcgaatgg caagttccga
     3421 tgcgtggtcg cgcgcgctga cgccccagca gttcggcgcg caggtgcgtc agcgaagttc
     3481 gcttcgagtg tgtacacggc tcaccatcac ggccgcctgg gtgcgccgct gcatgccgag
     3541 tttgccgagc aggctcgaca cgtagttctt gactgtcttc tcggcgagga agaggcgttc
     3601 gccgatctgg cggttcgtca gcccttcgcc gattaggtca aggatccgcc gctcctgatc
     3661 ggtcaacgac gacagcgggt cttcggctcg ggttccggtc cgcaaccgtt ccatcacctg
     3721 cgcggtcgtg ccggcgtcga gcatcgactg cccgcttgcg accgtgcgga tcgcgttcac
     3781 caggtcaccg ccgcggatct gcttcagtac gtacccggac gcacccgcca tgatcgcttc
     3841 gaacagcgcg tcgtcgtcgg cgaacgacgt gagcatcagg caggccaggt tcggcagctg
     3901 cgagcgaagt tcacgacaca cgacgacccc gtcgccatca gggaggcgga cgtcgagcac
     3961 ggcgacgtca ggcatcagcg tcggcactgc cgccagcgct tcggcggcgg tcgatccctc
     4021 gcccaccact actaggtcgg gcgcggtgtt gagcatgtcg cgcactccgc gccgaacgac
     4081 ctcgtggtcg tcgagaagga acaccctgat ctgccgggtt ccgaccggtt gggtggccgt
     4141 ctgcgtgccg atttgctgcg tgccctggct cagctcagtc atctcatgcg ccccaaggtt
     4201 tcgtggcggt ttggcgccgc tacccgagac actacgcttt gcgaggtatg accgcccatg
     4261 cccggagtgt tcgaagtagt ccggcgctca gctggtagct cgccaggtgc tcgatcgcga
     4321 cccaggcgac ctcggtcgcg tcgtccccag cgaccggaac gccgccggtc ggcgtcacct
     4381 cgaaatcccg gatccgataa gtcacgccgc ccggcccagg ccgcaccacc tcaccgacaa
     4441 ggcgcccgac ctcgacaacg agccccgttt cctcggcgac ctcgcgaacc agcgcctcct
     4501 gcgaggtctc gccaggttcg acccggccgc ccggtagcga ccaccttccg cgggagggtg
     4561 cgtgaccacg ccggatcagg agcagcgcgc catcgcgaat cgcgatggcg ccgaccgccg
     4621 ctacttccgc catggccacg agcctaggag tcgaggccgt gagccacctt gagtgcgttg
     4681 gaccggccgc gaggggtggg t
//
LOCUS       DASWVF010000107         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_385194, whole genome shotgun sequence.
ACCESSION   DASWVF010000107 DASWVF010000000
VERSION     DASWVF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385194"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VGD55_01845"
     CDS             <1..1094
                     /locus_tag="VGD55_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY0869113.1"
                     /translation="LAKYYNKSLAWNAGKTDVVMNMKGVGGQYDSFQNNQAALSYAQR
                     ALVRSTEASTEPAISAYPFQTELNIQGANWHYLTGAGYTDAASLVTTLMQNVSRNGSL
                     LLNLTQHGRGNLDTQCTQIAQDIGAWLKTNGEAVYASRPFEVSGEFSGATQTVAYTRN
                     KGNVYAVLFAWNGGAISLPGLKSGGATLGTVSKVEMLGSTVAMTFSQNAQGLTVTPGG
                     TVAAMAGISNQQLAAKMRVLRITHAKGWVNDDDPGSAAPGWLRTCNLGTGDYNNDLTT
                     SSTVGATWTTSFTGQGVTIYAPKQTGAGKIDIQVDGQSQAMADLSATGARQPQQAVST
                     VTGLAAGNHTLSIVNRGPGPVSVDAIVVQ"
     gene            1164..>1693
                     /locus_tag="VGD55_01850"
     CDS             1164..>1693
                     /locus_tag="VGD55_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869114.1"
                     /translation="MAALVLAGCGGYGKAITGTHGAGGTAGSGSGGTAGFLQVVVAPK
                     VNIDLDLLLVIKNSSSTAAMQQKFVTQLPTLMNVLEKLPNGLPNLHIAVVSTDMGAPG
                     DSVAEIGCKPYGDDGEFQSKPRGACVTTTLTNSATFISDVDGQKNFTDPIENVLQCIS
                     LLGNSGCGFEQHLAAI"
BASE COUNT          331 a          538 c          564 g          260 t
ORIGIN      
        1 tcctggcgaa atactacaac aagtcgctgg cgtggaacgc ggggaagacg gacgtcgtca
       61 tgaacatgaa gggcgtcggc ggccagtacg acagctttca gaacaatcag gccgcgctct
      121 cctacgcgca gcgcgcgctg gtgcgcagca cggaggccag caccgaaccc gccatcagcg
      181 cgtacccgtt ccagaccgag ctcaacatcc agggcgcgaa ctggcactac ctgaccggcg
      241 ctggatatac cgatgccgcg agcctggtga cgacgctgat gcagaacgtc tcacgcaatg
      301 gaagcctgct gctgaatctc acccagcacg gccgcggcaa cctggatacg caatgtacgc
      361 agatcgcgca ggacatcggc gcgtggctga aaacgaacgg cgaggccgtc tatgcgtctc
      421 ggcctttcga agtctccggc gagttttccg gcgccacgca aacggtggcg tacacgcgca
      481 acaagggcaa cgtgtatgcg gtcctcttcg cctggaacgg cggcgcgatc tcgctgccgg
      541 ggctgaagtc gggtggggcg acgctgggca cggtgtccaa ggtcgagatg ctcggctcca
      601 ccgtcgcgat gaccttctct cagaacgccc agggattgac ggtgaccccc ggcggaacgg
      661 tggcggcgat ggcggggatc tcgaaccagc agctcgccgc gaagatgcgc gttctgcgca
      721 tcacgcacgc gaaggggtgg gtcaatgacg atgaccccgg gtcggccgcg ccgggatggc
      781 ttcggacctg caacctcggc accggggact acaacaacga cctgacgacc agtagcaccg
      841 tcggggccac gtggacgacg tcgttcacgg gccagggcgt gaccatttac gcgcccaaac
      901 aaaccggcgc ggggaagatc gacatccagg tcgacggcca gtcgcaggca atggcggatc
      961 tgtcggcgac gggcgcgcgc cagccgcaac aagccgtatc gaccgtgacc ggcctcgccg
     1021 cgggcaacca cacgctcagc atcgtcaatc gcggtcccgg gccggtgtcg gtcgacgcga
     1081 tcgtcgtcca gtagcgagag ggccccggcc ggcggtaccg tggtccgatg gttggtcgga
     1141 cgaagcgaca cgttgttggc gtggtggccg cgctggtgct cgcggggtgt ggcggatacg
     1201 ggaaagccat cacaggcact cacggcgccg gcggaacggc cggaagcggc tcggggggga
     1261 ccgccggctt tctgcaggtg gtggttgccc caaaggtcaa catcgacctg gacctgctgc
     1321 tcgtcatcaa gaactcttcg tcgaccgctg ccatgcagca gaaattcgtc acgcagttgc
     1381 ccacgctcat gaatgtgctc gagaagctgc cgaacgggtt gccgaacctg cacatcgccg
     1441 tcgtgtcgac ggacatgggc gcgcccggcg attcggtcgc cgagatcggc tgcaagccct
     1501 acggcgacga cggggagttt cagtcaaagc cgcgcggcgc atgcgtgacg accacgctga
     1561 ccaacagcgc gaccttcatt tcagacgtcg acggccagaa gaacttcact gacccgatcg
     1621 agaacgttct tcaatgcatc agcctgctcg gaaattctgg ctgcgggttc gagcaacatc
     1681 tggcggccat cga
//
LOCUS       DASWVF010000108         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_181364, whole genome shotgun sequence.
ACCESSION   DASWVF010000108 DASWVF010000000
VERSION     DASWVF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181364"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VGD55_01855"
     CDS             <1..1978
                     /locus_tag="VGD55_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0869115.1"
                     /translation="GLMTQAREVLPYGSWPSPITAADVAGGGIRLSFPALVAGVGAQN
                     PPDVCWLEGRPTEGGRQVVVSAARGDLLPAPWNARTRVHEYGGLSWLPLPDGLLFTEW
                     SDQRIYRVSDTTDPRPLTPVPAIPAALRYADPVISPDGREVWCVREEHHSESPSDVTR
                     AIVGVPLDGSAADDPAKIRVIVSGSQFFAYPTPSPDGEQLAWIAWNHPDMPWDSTELR
                     VGDLTAGTVHEERTVLGGEHESVLEPRWADSGHLVAVSDRSGWWNLYRVDASGIKSPT
                     VLAPRDEEFAGPLWQLGYHHYDVLDDGRLVVLHGGGTQKLGVLDPTTGSLDVVELPYT
                     HWHPSIGVHGRYLVGVAGSAVLPDTVVRVDLHTRDYVELRHSVEELPPREYLPQPYTT
                     TFIGPTGRDVHAHVYAPHNPIAQAPEGERPPFIVFVHGGPTASSPPSLDLEYAYFTSR
                     GIGVIDVEYGGSTGYGREYRERLRGQWGIVDVEDCVAAVHALAESGQADAARLAIRGG
                     SAGGWTTLAALTRTTAFAAGTSYYGVAELLKFAHDTHDFESRYLDGIVGPLPAAHDLY
                     VERAPLSHVADVSCPVLLLQGLEDKVVPPSQAEMFRDALVVKGIPHAYIAFEGEQHGF
                     RKAETIIASLEAELSFYGQIFGFTPPGIALLQLS"
     gene            complement(2020..2454)
                     /gene="arr"
                     /locus_tag="VGD55_01860"
     CDS             complement(2020..2454)
                     /gene="arr"
                     /locus_tag="VGD55_01860"
                     /inference="COORDINATES: protein motif:HMM:NF033144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEY0869116.1"
                     /translation="MTTMRAPVPFEVYQPGVYLHGTKADLAIGELLAPGRESNFEPGR
                     MMNYIYFTATLDAATWGAELAAGGDRGRIYIVEPTGGFEDDPNVTDKRFPGNPTQSFR
                     SVEPVRVVGEIPDWVGHPPAVVDAMRERIAVAMGRGTSPIID"
     gene            complement(2481..2879)
                     /locus_tag="VGD55_01865"
     CDS             complement(2481..2879)
                     /locus_tag="VGD55_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869117.1"
                     /translation="MTQQMKNAARKHATLATLAILGGGISAAIWAGGSHGWAVGVAAF
                     YLVCCVALFAWSRGDGDVAAILRLSGDERQTMLDLRATAATGAATVVFCLGGAIVSLA
                     RGGSGNPWTLICAVAGFAYVIALGWSRHHH"
     gene            complement(2885..3085)
                     /locus_tag="VGD55_01870"
     CDS             complement(2885..3085)
                     /locus_tag="VGD55_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0869118.1"
                     /translation="MQNKVRQLRTASGLSQADLGAKLGVSRQTINAIEVGRYLPSLPL
                     AIGLARFFSSAVEDLFFDADPR"
     gene            3275..>4761
                     /locus_tag="VGD55_01875"
     CDS             3275..>4761
                     /locus_tag="VGD55_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0869119.1"
                     /translation="MSEAVLHETALLRRVRESVIGDDQVMPGPYGPRRVTYADYTASG
                     RALSFIEDFIRDEVLPRYANTHTESSGTGLQTTRLREDARRIIRDALHGDDETVVIFA
                     GSGCTGAIDKLIGILGFRIPAGLDDRYHLSDTIPAAQRPVVFIGPFEHHSNELPWRES
                     IADVVVIPQDADGHIDAAALEMELDNYGDRVVKIGSFSAASNVTGIVSDTYAISALLH
                     RHGALSFWDFAAAAPYVDINMYGGTDPLAYKDAIFLSPHKFIGGPSTPGVLVVRRDLM
                     RNRVPDVPGGGTVAYVNPTEHRYLTDPAQREEGGTPAIVESIRAGLVFQLKQAVGIDV
                     IRAHEEHYLRRAVTAWRAEPAIEILGDLDAERLSIVSFVVRAPSGRYLHHNFVVALLN
                     DLFGIQSRGGCSCAGPYGHRLLGIDLDRSHEFEREIAGGCEGIKPGWVRVNFNYFISE
                     PVFTYIVDAVALVARDGWRLLTDYCFDPATGLWRHRRGLIEPPLRL"
BASE COUNT          826 a         1609 c         1478 g          848 t
ORIGIN      
        1 tggactcatg acccaagcac gcgaagtgct cccgtacggc tcctggccgt caccgatcac
       61 ggccgcggac gtcgccgggg gtggcatccg actcagcttt cccgcgctcg tcgccggcgt
      121 gggcgcccag aacccgcccg acgtctgctg gctcgaaggc cggcccaccg aaggtggccg
      181 ccaggtagtc gtgtccgcag cccgcggcga cctgctgccc gctccctgga atgcgcgcac
      241 ccgagtgcac gagtacggcg gcctttcgtg gctcccgctg cccgacggcc tgctgttcac
      301 cgagtggtcg gaccagcgga tctaccgcgt cagcgacaca acagatccgc gcccattgac
      361 accggtcccg gctattccag cggcgctgcg gtacgcagac ccggtcatct cccccgacgg
      421 ccgcgaggtg tggtgcgtcc gcgaggagca ccacagcgaa agtccgtccg acgtgacgcg
      481 cgccatcgtc ggcgtcccgc tcgatggatc tgcggccgat gatcccgcga agattcgcgt
      541 cattgtctct ggcagtcagt tcttcgccta tccaacgcca tcaccggacg gcgagcagtt
      601 ggcgtggatc gcctggaacc accccgacat gccgtgggat tcaaccgagc tgcgcgtcgg
      661 ggatctcact gccggaaccg tgcatgagga gcgcacggtg ctcggcggcg aacacgagtc
      721 ggtgcttgag ccgcgatggg ccgacagcgg gcacctggtt gccgtcagcg accggtcagg
      781 ctggtggaac ctttatcgcg tcgatgcatc gggtatcaaa tccccgaccg ttctcgctcc
      841 gcgagacgag gagttcgccg gcccgttgtg gcagctcggg tatcaccact acgacgtgct
      901 cgacgatggc cggctggtcg tactgcacgg tggcggtacg cagaagctcg gcgtccttga
      961 tccgacgacg ggcagcctgg acgtcgtcga gttgccatac acccattggc atccgtcgat
     1021 tggtgtgcac gggcgatatc tcgtgggagt cgccggcagt gcggtcctcc cggacactgt
     1081 ggtcagggtc gacctccaca cgcgcgacta cgtcgagctg cgtcattcag tcgaggagtt
     1141 gcctccgcga gagtacctgc cgcagcccta cacgacgacg ttcatcggac cgaccggccg
     1201 cgacgtgcat gcccacgtgt acgcgccaca caaccccatc gcgcaggcgc ccgagggcga
     1261 gcggccgccg ttcatcgtct tcgtgcatgg cggccccacc gcatcgtccc ccccatcgct
     1321 cgacctcgag tacgcgtact tcacgagtcg cggcatcggc gtgatcgatg tcgaatacgg
     1381 cggctcgacg ggctacggtc gcgagtaccg cgaacgcctg cgcggccaat ggggcatcgt
     1441 cgacgtcgag gactgcgtgg ccgccgtcca cgcccttgct gaaagcggtc aggcggacgc
     1501 cgcgcggctg gcaattcgcg gaggcagcgc aggtggttgg accaccctcg ctgcgctcac
     1561 tcgcacgacg gcattcgcag cgggtacgtc gtactacggc gtcgcggaac tgctcaagtt
     1621 cgcgcacgac acgcacgact tcgagtcgcg ctatctcgat gggatagtcg gtccgcttcc
     1681 cgctgcccac gacctctacg tcgagcgcgc ccccctttcc cacgtcgccg acgtttcctg
     1741 ccccgtgctg ctattgcaag gtctcgaaga caaggtcgtg ccgccgtcgc aagccgagat
     1801 gttccgcgac gcgttggtag taaagggaat ccctcacgcg tacatcgcct tcgaaggcga
     1861 acaacacgga tttcggaagg ccgaaaccat catcgcgagc ctcgaagcgg agctgtcgtt
     1921 ctacggccag atattcggct tcaccccgcc tgggatcgca ctgctccagt tgtcgtaggg
     1981 cttgcgaatc ggacgaagac agtgttgctc cgacgatgct cagtcgatga tcgggctggt
     2041 gccccgaccc atggcgacgg caatacgttc acgcatcgca tccacgactg cgggcggatg
     2101 ccccacccag tccggtattt cgcccacgac tcgaacgggc tcaacgcttc gaaacgactg
     2161 cgtcggatta cccgggaacc tcttgtcggt gacgttggga tcgtcctcaa agccgccagt
     2221 cggctcaacg atgtagatac gcccgcggtc gccgccagct gcaagttccg caccccacgt
     2281 cgccgcgtca agcgtcgcgg tgaagtagat gtagttcatc atccgaccag gctcgaaatt
     2341 cgactctcgg cccggcgcca gtaactcgcc aatcgcgagg tcagccttag tgccgtgcaa
     2401 gtacacgcca ggttgataaa cctcaaacgg caccggtgcc cgcattgtgg tcatgggtcg
     2461 aggctagccg aggtaagccg tcagtggtgg tgtcggctcc acccgagcgc gatgacgtaa
     2521 gcaaacccgg caacggcgca gatcaacgtc cacgggttgc cacttccgcc gcgcgcgagg
     2581 ctgacgattg cgccgcccaa gcagaacacg acggtggcag ctccagttgc cgccgtcgcg
     2641 cgcaggtcga gcattgtttg ccgctcatcc cctgacagcc gaaggatcgc cgcgacgtcc
     2701 ccgtcaccgc gtgaccatgc gaaaagcgcc acgcaacaca ccaggtagaa ggccgctacg
     2761 ccgaccgccc atccgtggct tccgccagcc cagatcgctg cggagatgcc gccgccgagg
     2821 atcgccagtg tcgccaatgt ggcgtgcttt cgagctgcgt tcttcatctg ttgggtcatt
     2881 gtcgtcatct cggatcggcg tcgaagaaca ggtcttcgac agcgctggaa aagaatcgtg
     2941 cgagcccgat cgccaagggc agcgacggca agtaccggcc gacctctatg gcgttgatgg
     3001 tctgccgcga cacgccgagc tttgcgccga ggtcggcctg cgacagtccg cttgccgtac
     3061 gaagctggcg caccttgttc tgcacaatgt aaagctagct tgtcatttcg agcatgtcaa
     3121 ggttgcttta catacgcggg ctcgaggacc ggcttgaaca tccgctgccg ctggccttgt
     3181 ttcgacagcg gcgtcgactg gcactcgggt aacgtcgggt catggacctg tatcggcgcc
     3241 gcacgcatcg atgcccggga ccgaggagtt gcacatgagc gaggcggttc tgcacgagac
     3301 agcattgttg cgtcgggtca gggagtcggt gatcggcgac gaccaggtca tgcccgggcc
     3361 ctacgggccg cgccgcgtta cctacgccga ctacaccgcg tccggccgcg cgctgagttt
     3421 catcgaggat ttcatccggg acgaggtctt gccgcgttac gcgaacacgc acaccgagtc
     3481 gagcggaacc gggctacaga caacccggct gcgcgaggac gcccgccgga ttattcgcga
     3541 cgcgctgcac ggcgatgacg agacggtcgt gatcttcgcc ggatcgggct gcacgggcgc
     3601 gatcgacaag ctcatcggca ttctcggctt tcgtatcccc gccgggttgg acgaccgcta
     3661 tcacctcagc gacacgatcc ccgcggcaca acggcctgtc gtgttcatcg gaccgttcga
     3721 acatcactcg aacgaactgc cgtggcgcga atcgatcgcc gacgtcgtcg tcatcccgca
     3781 ggatgccgac ggccacatcg acgcggccgc tctggaaatg gaactggata actacggaga
     3841 tcgcgtggtg aagatcggat cgttttccgc agccagcaac gtcaccggaa tcgtcagcga
     3901 cacctacgcg atatcggcgc tgctgcaccg gcacggcgcg ttgtcgttct gggattttgc
     3961 cgccgccgca ccctacgtcg acatcaatat gtacggcggc accgatccgc tcgcgtacaa
     4021 ggacgcgatc ttcctcagcc cgcataagtt catcggcggc ccgagcacac ccggcgttct
     4081 ggttgttcgg cgcgacttga tgcgcaatcg ggtacccgat gtccccggcg gcggcacggt
     4141 tgcctacgtg aaccccaccg aacaccgcta cctcaccgac ccagcgcaac gcgaggaggg
     4201 tggcacgcct gcgatcgtcg aatcgatccg cgccggactc gtgttccaac tcaagcaggc
     4261 cgtcggcatc gacgtcatcc gcgcgcatga ggagcactac ctacgccgtg ccgtcaccgc
     4321 ctggcgcgcc gagcccgcga tcgagattct cggcgatctc gacgccgaac gactgtcgat
     4381 cgtgtcgttc gtggtgcgag ctccgagcgg acggtacttg catcacaact tcgtcgtcgc
     4441 cctgctcaac gacttgttcg gaatccaatc tcgcggaggt tgctcgtgcg caggcccgta
     4501 cgggcaccgg ttgctcggca tcgacctcga ccgatcgcat gagttcgagc gcgagatcgc
     4561 cggcggctgt gaaggaatca agcctggctg ggtcagggtc aacttcaact acttcatctc
     4621 cgagccggtt ttcacctaca tcgtggacgc cgtcgcgctg gtcgcgcggg acgggtggcg
     4681 gctcctcacc gactactgct tcgaccccgc cactgggcta tggcgtcacc ggcgtgggtt
     4741 gatcgaaccg ccgctgcgtc t
//
LOCUS       DASWVF010000109         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_206962, whole genome shotgun sequence.
ACCESSION   DASWVF010000109 DASWVF010000000
VERSION     DASWVF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206962"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VGD55_01880"
     CDS             <1..1347
                     /locus_tag="VGD55_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEY0869120.1"
                     /translation="QFPDLLPQLQQSSARVSQRMTELNCEIIDVGFISDAEEGARAAE
                     QLRVAGCDLIVGFLTTYMTATMLVPIAQRSGAPVLLINLQPTESMDHATFDTGQWLAY
                     CGACPLPEMANAFERCGVPFRSVSGYLEDPRAWERISGWSKAAGVRGTLRNARHGSMG
                     HLYPGMLDVSTDLTMVTSHLGGHMEVVEFDDLRVRVDAVTDDEVAAAIELAREVFELD
                     GSVDPVDFEWAARVSVGLDRLVDDFSLDSIAYYHRGLEGEKHERLGAGMILGASLLTA
                     RGIPVVGEYELRTSLATLVMDRLGAGGSFTEFQALNFRDGVVEMGHDGPAHLAISDQR
                     PLLRGLGVYHGKRGWGVSVEFDVKHGPVTAFGIGQRRDGSYVGIAAEGEVVGGPLLRV
                     GNTTSRVDFGCDPGVWTDAWSGSGVGHHWALGTGHRIGELRATAELLELELVEVRP"
     gene            1353..>2485
                     /locus_tag="VGD55_01885"
     CDS             1353..>2485
                     /locus_tag="VGD55_01885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEY0869121.1"
                     /translation="MSVAAVDLGASSGRVLVGQVGQGRLELHEVNRFPNVPVRVAGVL
                     QWDILALYRGVLEGLRAAGREFGHLDSIGIDSWAVDYGLVDASGALLANPVHYRDERT
                     AGVMDRVLQIVPATELYKTTGIQFLPINTVYQLVAAMTTPVMAVAKTLLLIPDLMSFW
                     LTGDIGAETTNASTTQLLDVSTGQWATGVMNELGIDPMLFPALRQPGAPAGRLRDDVL
                     AETGLVGPVVVTTVGSHDTASAVVGVPAVTPNFGYISCGTWSLVGLELDQPVLMDAAR
                     LANFTNEAGVDGTTRFLRNVMGLWLLQECVRTWNSLGLPADLETLLVEAGRVPPFTAL
                     IDPDDPVFLPPGDMPARIANACERRGQTPPQSQAQTVRCVLDSL"
BASE COUNT          420 a          758 c          839 g          468 t
ORIGIN      
        1 cagttccccg atctgctccc tcagttgcag cagtcgtcgg cccgcgtctc gcaacgcatg
       61 acggagctga actgcgaaat catcgacgtc gggtttattt cggacgccga agaaggtgcg
      121 cgggccgcgg agcagttgag ggtggccggc tgcgatctga tcgttgggtt tctcacgacc
      181 tacatgaccg cgaccatgct cgtgccgatc gcgcaacgca gtggtgcgcc ggtcttgctg
      241 atcaacttgc aaccgactga gtcgatggat cacgcgacgt tcgacaccgg gcagtggctc
      301 gcctactgcg gtgcgtgccc gctgccggag atggcgaacg cgttcgaacg atgcggcgtg
      361 ccgtttcgat ccgtatcggg ctacctcgaa gatccgcgag cctgggaacg gatcagtggc
      421 tggagcaagg cggccggcgt gcgcggcaca ttgcgcaacg cgcggcacgg ctcgatggga
      481 catctctacc ccggcatgct cgacgtctcg actgacctca cgatggtcac gtcgcatctt
      541 ggtggacaca tggaagtcgt ggagttcgac gatctccggg tgcgcgtcga tgccgtcacc
      601 gacgacgagg ttgcggcggc gatcgaactc gcgcgagaag tgttcgaact cgacggcagt
      661 gtcgatccgg tcgatttcga atgggctgcg cgggtctcgg tcgggctcga ccggctggtc
      721 gacgacttct ccctcgactc gattgcctac taccaccgcg gccttgaggg tgagaagcac
      781 gagcgccttg gcgccgggat gattctgggc gcttcgctgc tgactgcgcg cggcattccg
      841 gtggtcggcg agtacgaact gcgaacgtca ttggcgacgc tcgtgatgga tcgtctgggc
      901 gcaggcggat cgtttaccga attccaggcg ctgaacttcc gtgacggcgt cgtcgaaatg
      961 ggacacgacg ggcccgccca cttggcgatc agcgatcagc gccctctgct gcgcggactc
     1021 ggggtctatc acggcaaacg cggctgggga gtgtcggtcg agttcgacgt gaagcacgga
     1081 cccgtcaccg cgttcggcat cggccagcga cgcgacggca gctacgtcgg catcgctgcc
     1141 gagggcgaag ttgtcggcgg tccgctgttg cgggtgggca acacgacatc gcgtgtcgac
     1201 ttcggttgcg accccggcgt atggaccgac gcgtggagtg gctccggcgt cggtcaccat
     1261 tgggcgctgg gcaccggcca tcgcatcggc gaactgcgcg ccactgctga gctactcgaa
     1321 ctcgagcttg tcgaggtccg cccttgacgc aggtgagcgt cgcggcggtc gacctcggag
     1381 cgtcgagcgg gcgggtattg gtcgggcagg tcggccaggg tcgtctcgaa ttgcacgagg
     1441 tcaatcgatt ccccaacgtg ccggtgcgag tcgcgggtgt gctgcagtgg gacatcctcg
     1501 ccttgtatcg aggagtgctc gagggcctgc gcgccgcagg ccgggagttt ggccacctcg
     1561 acagcatcgg catcgattcg tgggccgtcg actacgggct ggtcgacgca tctggagcgc
     1621 tgctcgcgaa ccccgtgcat taccgcgacg agcggaccgc tggtgtcatg gaccgcgtgc
     1681 tccagatagt gcctgccaca gagctgtaca agaccacggg cattcaattc ttgccgatca
     1741 acacggtgta ccaacttgtc gctgcaatga cgacaccagt gatggcggtg gcaaagacgc
     1801 tgttgctgat tccagatctg atgagcttct ggcttacggg cgacatcggc gccgagacta
     1861 ctaatgcctc gactacacag ctactcgacg tcagcaccgg ccaatgggcg acgggcgtca
     1921 tgaatgagct cggcatcgac ccgatgctgt ttccagcgtt gcgccagccg ggcgcgccag
     1981 ccggacgcct tcgcgacgac gtgttggccg agacaggcct agtcggaccg gtcgttgtca
     2041 ccacggtcgg ctcgcatgac acggcgtcag cagttgtcgg agtgccggcg gtgacaccga
     2101 attttggcta catctcgtgc ggcacatggt cactggttgg actcgaactt gatcagccgg
     2161 tactgatgga tgcagcgcgc ttggcgaact tcaccaatga ggccggagtc gatggcacca
     2221 cgcgtttcct gcgcaatgtc atggggctgt ggctgttaca ggaatgtgta cgcacctgga
     2281 attcgctcgg gttgcctgcc gacctcgaaa cattgctggt ggaggcaggc cgggtgccgc
     2341 cgttcacagc gctcatcgac cccgacgacc cggtgttcct gccgccaggc gacatgccgg
     2401 cacgcatcgc gaacgcgtgc gaacggcgcg gacaaacgcc accgcaaagt caggcgcaga
     2461 cggtgcgttg cgtcctcgac agcct
//
LOCUS       DASWVF010000110         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_207022, whole genome shotgun sequence.
ACCESSION   DASWVF010000110 DASWVF010000000
VERSION     DASWVF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207022"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..>2484
                     /locus_tag="VGD55_01890"
     CDS             184..>2484
                     /locus_tag="VGD55_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0869122.1"
                     /translation="MDAAERLRPFIAGFVVDWLRETPAERHRVAQGTLVFVDISGFTR
                     LTERLASKGKIGAEEMSDILDVTFAALLAVAYDHGAWLVKWGGDAVLLLFEGDQHAER
                     ACTAAHEMRLTMRRIGRLQTSVGEITLRVSIGVHTGDFDFYLVGSLHHELLISGRAAS
                     QTVAMETTADAGEIVVSAPTAAALRSRHVGAAKGDGFLLTRSPGTPARLELRTRDLTG
                     VDLESCLPEVTRNHLLAGATDGEHRQIAVAFVEFSGIEAVLEKGGTAAVADAVDHVVR
                     ACQEAAQRHEVTFWETDVGVDGGKVMLVSGAPRSAGHDEDRMLAVVREVIDAGGDLSL
                     RAGVNSGRVYAGEFGPPFRRTYSVKGDAVNLAARLMSKAGPGEIYAADAVISRARSAF
                     ATDALPPISVKGKARPVVAHRVRHAQDTDAGTLRVDLQFAGREEELSALQAALSEARQ
                     GNGEVWEVSGEAGIGKSRLLAELVARADAVTVVSVVCDQYHSSTPYAPFRRLLRRLAG
                     IAEDADPLVAGDGLLEVVARAAPSLLAWTPLLAAVLAVQVEPTPETDALEAKFRKTRQ
                     EEEAGRFLAALVPGPLLLVVEDAHLMDDASLDLLRHLAELASGRPWLIVATTVSVRDS
                     KTIFDDAVAPHRMPLGTLGTDAVEALIKRATEDRPLPPHEIALLNQRAGGNPLFVQEL
                     LGATRAGGTVSALPDSVEGLMAVQVDRLKPHERRMLRIASVLGVRVDRQVLDEMLRIE
                     DVDIDVTRNVLDDFLEPDGDGLRFRHS"
BASE COUNT          399 a          800 c          854 g          431 t
ORIGIN      
        1 tcgcgtatct acaacttcaa aacgggcgcc gagaccgtga cggtctcgat tccgttccaa
       61 gccggccgcg gcgaccccaa actctggtaa acccggccca cgtcgcgcgg gctcccgccg
      121 gtgagccggt agcttgggag cccgcggggt gaggtctgca cccgcagaag ggtgggccgc
      181 gccatggatg ccgccgagcg gctgcgaccc ttcatcgcgg ggttcgtcgt cgactggctg
      241 cgggagacgc cggcggagcg gcaccgcgtt gcgcaaggaa cgctcgtctt cgtcgatatc
      301 tccggtttca ccaggctgac cgaacggctt gccagcaagg gcaagatcgg tgccgaggaa
      361 atgagcgaca tcctcgacgt caccttcgcc gcgctgctcg ctgtcgcgta tgaccacggt
      421 gcgtggctgg tgaagtgggg cggcgacgcg gttctgctgc tgttcgaggg cgaccaacat
      481 gccgagcgcg cgtgcacggc cgcgcacgag atgcggctga cgatgcggcg tatcggtcgg
      541 ttgcagacct ccgtcggcga gatcactctt cgggtttcca ttggcgtgca caccggcgac
      601 ttcgacttct acctggtcgg cagcctccac cacgaactcc tcatcagtgg tcgcgctgcc
      661 tcgcagacgg tggcgatgga aacgacggcc gatgcgggcg agatcgtcgt cagcgcgccg
      721 acggccgcgg cacttcggtc gcgacacgtc ggggcggcga agggcgacgg attcttgctg
      781 actcgctcgc cgggcacgcc ggcgcgacta gaactgcgca cgcgagacct gaccggtgtc
      841 gacctggagt cgtgcctgcc cgaggtcacc cgcaaccact tgctggcagg ggcgaccgat
      901 ggtgagcatc gccagatcgc ggtcgccttc gtcgaattct ctggcatcga agctgtcctc
      961 gaaaagggtg gaacggccgc tgtagccgat gcggttgacc acgtcgtccg cgcatgtcag
     1021 gaagccgcgc agcgacatga ggtgacgttc tgggaaaccg atgtcggcgt cgacggcggc
     1081 aaggtgatgt tggtgagtgg cgcacctcgc agcgccggtc acgacgagga tcggatgctc
     1141 gctgtcgtgc gcgaagtgat cgatgcaggt ggtgatctca gcttgcgcgc cggtgttaac
     1201 agcgggcggg tgtacgccgg tgagttcggg ccgccatttc ggcgcacata ctcggtcaag
     1261 ggcgacgccg tgaacctcgc ggcgcggctg atgtcgaagg caggtccggg tgaaatctat
     1321 gccgccgatg ccgtaatttc gcgtgcccgc agcgctttcg ccaccgacgc gctgccgccg
     1381 atatcggtga aaggcaaggc ccggccggtc gtcgcgcatc gtgtcaggca cgcgcaagac
     1441 accgacgccg gaacgctgcg ggtcgatttg cagttcgccg gtcgcgaaga agagttgagc
     1501 gctttgcagg ccgccctcag cgaagcgcgg cagggtaacg gtgaggtgtg ggaggtctcg
     1561 ggcgaagccg gcatcggcaa gtcgagactc ctcgccgagc tcgtcgcgcg cgccgacgca
     1621 gtcacggtcg tgagcgtcgt gtgcgatcag taccactcga gcacgccgta cgcgccgttt
     1681 cgccgtctgc tacggcgact ggccggaatc gccgaggacg ccgacccgct cgtcgccggt
     1741 gatgggcttc tcgaggtggt cgcgagagcg gcaccaagcc ttcttgcctg gacgccgctc
     1801 cttgccgccg tccttgcagt gcaggtcgag ccgactcccg aaaccgacgc cctcgaagcg
     1861 aaattccgca agacgcgtca ggaggaggag gccggtcggt tcctcgccgc actcgtgccc
     1921 ggcccgctgc tgctcgtcgt cgaggacgcc catctcatgg acgacgcatc gctggatctg
     1981 ctgcgtcatc tcgccgaact cgccagcggc cgaccgtggc tcatcgttgc gaccacagtg
     2041 tcggtccgcg attccaagac aatcttcgac gacgctgtgg cgccacaccg catgccgctg
     2101 ggtaccctcg gcaccgacgc cgtcgaggcc ctgatcaaac gtgccaccga ggatcggccg
     2161 ctccctccgc acgagatcgc gttgctcaat cagcgcgcgg gtggcaatcc gctgttcgtg
     2221 caggagttgc tcggcgccac gcgcgcgggc ggcaccgtgt cggcgctgcc cgattcggtc
     2281 gaaggtctga tggccgtgca ggtcgacagg ttgaagccgc atgagcgccg catgctgcgc
     2341 atcgcctcgg tgctcggagt gcgtgtcgat cggcaagtac tcgacgagat gttgcgcatc
     2401 gaagacgttg atatcgatgt cacccgcaac gttctcgatg attttctcga gccggacggc
     2461 gatgggttgc gctttcggca cagc
//
LOCUS       DASWVF010000111         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_130627, whole genome shotgun sequence.
ACCESSION   DASWVF010000111 DASWVF010000000
VERSION     DASWVF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130627"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGD55_01895"
     CDS             complement(<1..242)
                     /locus_tag="VGD55_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869123.1"
                     /translation="MTWRGLDRLIDDIDEVALYEVDVVKPVFQNRNADADGENENSEE
                     DRRTIEQSAGKDPDDAKCASHHQPLQLLTQITDRTA"
     gene            complement(267..554)
                     /locus_tag="VGD55_01900"
     CDS             complement(267..554)
                     /locus_tag="VGD55_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY0869124.1"
                     /translation="MTAYWISIYPEVIDESKFAAYAKLSRPALEAAGGTFVARGLPEL
                     VYEGGKMTRVVVIEFESVEAARNAHESAAYQEALAALDGGAVRDLRIVPGI"
     gene            complement(653..1663)
                     /locus_tag="VGD55_01905"
     CDS             complement(653..1663)
                     /locus_tag="VGD55_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0869125.1"
                     /translation="MSPALIALIVVAIFVLLVVGRTVRIVPQARAGIVERFGRYNKTL
                     KPGLNIVVPFVDRLRPLLDLREQVISFPPQPVITEDNLVVGIDTVLYFQVTDARAATY
                     EIANYIQAIEQLTVTTLRNVAGGMDLEKTLTSREEINSQLRVVLDEATGKWGIKVNRV
                     ELKSIDPPASIKDSMEKQMGADRDKRAAILTAEGFKQSQILTAEGEKQSAILKAEGQA
                     QAAVTQATADAKAQALRAEGQAQAISTVFQAIHEGKPDAELLAYQYLQMLPQIAKGDA
                     NKLWIVPSEMGKALEGLGSILGGMGGMGGNASNGSAAVPATPAAPKAVAPKALDAGEN
                     AG"
     gene            complement(1667..2098)
                     /locus_tag="VGD55_01910"
     CDS             complement(1667..2098)
                     /locus_tag="VGD55_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0869126.1"
                     /translation="MSAWLVWTIVAAVCAGAEAMTLTLVLGFVSVAAIAALVVAVAGG
                     PVPLQFGAFIAGSVALLGVARPIAKSHLRTPAELKSGVDALVGKRALVLERVDAHNGQ
                     VKIGGEIWTARAYMEDQVLEPGTSADVVKIQGATALVYGTE"
     gene            complement(2138..2929)
                     /locus_tag="VGD55_01915"
     CDS             complement(2138..2929)
                     /locus_tag="VGD55_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869127.1"
                     /translation="MQATGDVISMQGVGVVRDGATLLDRIDWAVDHDQRWVILGPNGA
                     GKTTALRVASTRLFPSRGTVDILGERMGAVDVFELRPRIGLSSTALGERIPAKEKAKD
                     VVLTASYGMVGRWRERYDIWDDTRADVVLRQLGCAHLADRTYGTLSEGERKRVQIARA
                     LMTDPEMLLLDEPAAGLDLGGREDLVARLASIAADPGAPTTVLVTHHVEEIPIGFTHA
                     LLLRGGQVVAAGPLHDSMTEESLGYTFGVALHLDYREGRFSARAR"
     gene            2999..3988
                     /locus_tag="VGD55_01920"
     CDS             2999..3988
                     /locus_tag="VGD55_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869128.1"
                     /translation="MPMPHEDFAGDPPDDRAGDPVSEPVADPELCGRFAAWFAAALNG
                     RVESTAALTAIESGSDGHTVAGWHDWWAGTNGAGLVARLPEAGQAIVEFTIADVFDRL
                     LDIGVQRVSLVLPVAGDPLGLVGTGTFSVAALDAECGVLLVTDHARLGLIPTHDRRGS
                     SYRGWRWQVYVEPIDFVVDAGPVDPFAPVGTLPPAEPEQVIEQADRALARALHAATHE
                     LAALDLAHWRPEVAAGRKEAEAALRAAGQRMPPGWPPAARALAERALMLWRIVRVARA
                     DAGAASASGSQVRGEVLRGLSHAVREAAMVAYNLPLLTLPASRPASAVESDLR"
     gene            3985..5196
                     /gene="serB"
                     /locus_tag="VGD55_01925"
     CDS             3985..5196
                     /gene="serB"
                     /locus_tag="VGD55_01925"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0869129.1"
                     /translation="MSLERTLLVTLTGRDRPGVTSRLFTELARFPLVVVDTEQIVLRG
                     RLILGVLVDLAGADAAAELALRDGLSQAARDLGLEIEVTVGPDDDGLATPGPLHVTVL
                     GNPLLPAAMAGVAGCIASCGANIDRIVRLASEPVTCIEMDVSGADPDVLRAELNAQAA
                     LLQVDVAVQRSGLYRRAKRLVVMDVDSTLVRGEVIEMLAERAGCALEVAAITEAAMRG
                     ELDFEQSLRERVALLAGLSAAALTDVSESLVLTPGARTLVRTLKRLGYLVAIVSGGFT
                     QITDGLVDELGLDFATANTLDVVDGVLTGKLVGPIVDRAGKAAALERFAALAGVPLTQ
                     TIAIGDGANDLDMLGRAGLGVAFNAKPVVREAADTALNVPYLDAILYLLGITRDEINA
                     ADAEGPAFVRT"
     gene            complement(5468..>5941)
                     /locus_tag="VGD55_01930"
     CDS             complement(5468..>5941)
                     /locus_tag="VGD55_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0869130.1"
                     /translation="RGIMLAAFLAQPEQQPTVEWLAGGTFAVLLDAAATNGQVGVGRF
                     RVSEGEAPPYHKHLREDEIFMLISGTALLWCDDEEMELSEGGIVFLPRNVPHGYRITS
                     PEADLLMMCTPGGFESMFLQAGTLVTDPRQPTEPARNAMVKAAEDFGQVIVGPPR"
BASE COUNT          966 a         1962 c         1925 g         1088 t
ORIGIN      
        1 atcgcggttc ggtcggtgat ctgcgtgagc agctgaagtg gctgatggtg ggaggcgcac
       61 tttgcgtcgt cgggatcctt gccggcgctt tgctcaatgg tgcggcggtc ttcctcggag
      121 ttctcgtttt ccccgtctgc atcggcattg cgattttgaa ataccggctt tacgacatcg
      181 acctcgtaca gcgcgacctc gtcgatgtcg tcaatcaatc gatccaaccc gcgccatgtc
      241 acggtgtgga tccgctcctc gtccagttag atcccgggga cgatgcgcaa gtcccgcact
      301 gcgccaccat cgagtgcagc gagggcttcc tgataggcgg cactctcgtg cgcgttccgt
      361 gccgcctcaa ctgactcgaa ctcaatcacc acaacgcggg tcatcttgcc gccttcgtac
      421 accaactcag ggagcccgcg tgcgacgaat gtgccaccag ccgcctcgag cgccggccgg
      481 gacagcttcg cgtacgccgc aaacttcgac tcgtcgatga cctcggggta gatgctgatc
      541 cagtaagccg tcatgcgtcc atcttgccct gcgtccactg ctacccgggc atcgccgacg
      601 gcgggcctag ccgccgcggg ttccaagtcc caaccgattc gggggacgcc gatcagccgg
      661 cgttctcccc tgcgtcgagc gccttcgggg caacggcctt cggggcagcg ggcgtcgccg
      721 gaacagcagc tgagccgttc gacgcgttgc cgcccatgcc gcccataccg ccgaggatgc
      781 tgccgagacc ttcgagcgcc ttgcccattt cactcggcac gatccagagc ttgttcgcgt
      841 cgcccttcgc gatctgcgga agcatctgga ggtactggta cgccagcagt tcggcgtccg
      901 gcttgccttc gtggatggct tgaaagaccg tgctgatcgc ctgggcttga ccctctgctc
      961 gtagcgcttg cgccttggcg tcagcggtcg cctgggtcac ggccgcctgc gcctgccctt
     1021 cggccttgag gatcgccgac tgcttctcgc cctcggcggt gaggatctgt gactgcttga
     1081 aaccctcagc cgtcaggatc gcggcgcgct tgtcacggtc ggcgcccatc tgcttttcca
     1141 tcgagtcttt gatcgaggcc ggcggatcga tcgacttgag ttcgacccgg ttgaccttga
     1201 tgccccactt gccggtggct tcgtcgagca cgacgcgcag ttgagagttg atctcttcgc
     1261 gtgacgtcag cgtcttttcc aaatccatgc caccggcgac gttacgaaga gttgtgacgg
     1321 tgagctgctc gatggcctgg atgtagttcg cgatctcata tgtagcagca cgcgcgtcgg
     1381 ttacctggaa gtacaagacg gtgtcgatgc cgacgacgag gttatcttct gtgatgacgg
     1441 gctgcggtgg gaaagagatc acctgctcgc gcaggtcgag caagggacgc aaacggtcga
     1501 cgaaaggcac cacgatgttg agtccgggct tgagggtctt gttgtaacgc ccgaaccgtt
     1561 cgacgatgcc ggcccgcgcc tgcggcacga tgcgcaccgt gcggccgaca acgagcagca
     1621 cgaatatcgc gaccacgatg agcgcaatga gtgccggact catgacctac tccgttccat
     1681 agaccagggc ggtagcgccc tggatcttca cgacgtcggc cgacgtcccg ggttcgagaa
     1741 cctggtcttc catgtaggcg cgcgcggtcc agatctcgcc gccgatcttc acctgaccgt
     1801 tgtgcgcgtc tacccgctcg agcaccagcg cgcgcttccc caccaacgca tcgacgccgc
     1861 tcttcagttc agccggtgta cgcagatgtg atttcgcgat cggcctggcc acgccgagca
     1921 gtgcgaccga tccggcgatg aaggcgccga actgtagagg aacggggccg cccgccacgg
     1981 cgacgaccag tgcggcgatc gccgcaaccg agacgaagcc gagaacgagc gtcagcgtca
     2041 ttgcttccgc gccggcgcag acggccgcca cgatcgtcca aacgagccaa gcggacataa
     2101 ctcgatcata ggccggtaat tcagccgatg caggccgtca ccgagcgcgg gcgctgaacc
     2161 tgccctcgcg gtaatcgagg tgcaacgcga caccgaaggt gtagccaagc gattcttcgg
     2221 tcatcgagtc gtgcagcggg cccgcggcga cgacctggcc gccgcgcagc aacagggcat
     2281 gcgtgaagcc gatcgggatc tcttcgacgt gatgggtcac caggacggtc gtcggggcac
     2341 cagggtctgc ggcgatcgag gcaagacgag cgaccagatc ctcgcgcccg ccaaggtcga
     2401 ggcccgcagc cggctcgtcg agcagcaaca tctcgggatc ggtcataagt gcccgcgcga
     2461 tctgcactct cttgcgctcg ccttcagaca gcgtgccgta ggtgcgatcg gccagatgtg
     2521 cacacccgag ttgcctcaac acgacgtcgg cacgggtgtc gtcccagatg tcgtaccgct
     2581 cgcgccaccg cccgaccatg ccgtaggacg cggtcaacac gacgtccttc gccttttcct
     2641 tggccggaat gcgctcgccg agcgccgtgc tcgagaggcc aatccttggg cgcagctcga
     2701 agacatcaac cgcacccatg cgctcgccga ggatgtcgac cgttccgcgg gacgggaaga
     2761 gccgggtcga ggcaacgcgc aaggccgtgg tcttgccggc gccgttcggg cctaggatca
     2821 cccaccgctg gtcgtgatcg accgcccagt cgatgcgatc gagcagcgtc gccccatcac
     2881 ggaccacacc gacgccctgc atcgagatca cgtcgccggt tgcctgcacg ctggaaactt
     2941 acctgcacga aacttaccgg gacgccgacg ggcgggcgcg gctgcttagg gtagtggagt
     3001 gccgatgccc cacgaggact tcgcaggcga cccgcccgac gaccgagcgg gcgatcctgt
     3061 gagcgagccc gtcgccgacc ccgagctgtg tggtcggttc gctgcctggt tcgccgccgc
     3121 gttgaacggt cgcgtcgagt ccaccgctgc attgacggcg attgaatcgg gcagcgacgg
     3181 acacacggtc gcgggctggc acgactggtg ggccggcacg aacggtgcgg gcctggtcgc
     3241 aagacttccg gaggccggtc aggcgatcgt cgaattcacc atcgccgacg tgttcgatcg
     3301 gctcctcgac atcggcgtgc agcgggtctc gctcgtgctg ccagtggcgg gcgatccgct
     3361 cgggctggtc ggtaccggca cgttctcggt cgccgcactc gacgccgagt gcggggtgct
     3421 gctagtgacc gatcacgcac ggctcggcct gattccgacg catgatcgcc gaggttcgtc
     3481 gtatcgcggc tggcgctggc aggtctacgt cgaaccgatc gacttcgtcg tcgacgccgg
     3541 gcccgtcgac cctttcgcgc ccgtcggaac cctgccaccc gctgagcctg agcaggtgat
     3601 cgagcaggca gaccgagcac tcgcgcgtgc gctgcatgcg gcgacacacg agcttgccgc
     3661 gctcgacctc gcacactggc ggcccgaggt ggctgccggc cgcaaggaag ctgaggcagc
     3721 gctacgcgct gcgggtcaac gaatgcctcc gggttggcca ccggccgcgc gcgctctcgc
     3781 cgagcgcgcg ctgatgttgt ggcggatcgt gcgcgtcgcg cgtgcggacg cgggtgccgc
     3841 ctcggcgtcc ggtagtcagg ttcgaggcga ggtattgcgc gggctgtcgc acgcggtgcg
     3901 cgaggcggcg atggtggctt acaacctgcc gctgctcacg ctgcccgcat cgcgacccgc
     3961 aagtgcggtc gagtctgacc tgcgatgagt ctcgagcgca cccttctcgt cacactgacc
     4021 ggccgcgacc gacctggagt gacgtcgcga ctgttcaccg aactagcgcg gttcccgctc
     4081 gtggttgtcg acaccgaaca gatcgtcctg cgcggtcggt tgatcctcgg cgtgctggtc
     4141 gatctcgccg gtgccgatgc cgcggccgag cttgctttgc gcgacggatt gtcgcaagct
     4201 gcccgtgatc tcggtctcga gatcgaggtc accgtcgggc cagacgacga cggtctagcg
     4261 acgccaggcc cgctgcatgt cacggtgctc ggcaacccgc tgctgccagc cgcgatggca
     4321 ggagtcgccg gatgtatcgc gagttgcggc gcaaacatcg atcgcatcgt gcggctcgcg
     4381 agtgagcccg tcacctgcat cgagatggac gtctcggggg ctgatcccga cgtgttgcgc
     4441 gccgaactga acgcgcaagc cgcgttgctt caagtcgacg tggcggtgca gcgcagcggt
     4501 ttgtaccgac gagcgaaaag acttgtcgtg atggacgtcg attcgacgct cgtgcgtggc
     4561 gaggtcatcg agatgcttgc cgaacgtgcc ggttgcgcac tcgaggtggc cgccatcacc
     4621 gaggcagcta tgcgcggcga gctcgatttc gagcagtcgc tgcgcgagcg agttgcgttg
     4681 ctcgcagggc tttccgcggc tgccctgaca gacgtgagcg aatcgctggt cctcacgccg
     4741 ggcgcccgca cgttggtgcg aacgttgaag cgcctcggtt acctcgtcgc gattgtgagc
     4801 ggaggattca cccagatcac cgatggcctc gtcgacgaac tcggcctcga cttcgccact
     4861 gcgaacaccc tcgatgtcgt tgacggcgtg ttgacaggca agctggtcgg accgatcgtc
     4921 gaccgcgccg gcaaggctgc ggcactcgag cggttcgcag ctttagcggg agttccgttg
     4981 acgcagacga tcgcgatcgg cgacggtgcc aacgacctcg acatgctcgg gcgcgcgggc
     5041 ctcggcgttg ccttcaacgc gaagcccgtc gtgcgcgagg ccgctgacac cgcgctcaac
     5101 gtcccttacc tggacgcgat cctctacctg ctcggaatca cgcgagacga aatcaatgcg
     5161 gcggacgccg aggggccggc gttcgttcgt acgtgagcgt gccgctgctc gggcgcgacc
     5221 tcagcgcgct tggtttttgc gccctcttct gggtatttca gcaggaccca catcggtagg
     5281 cccgctgcga tcagagtcat cacgacaccg agcggaatgt tcaaccagaa ccagttcatc
     5341 gtttcggcgc ctaggtcggg tacggcgccg tctcacctcc agagctcaac ctgccggtcg
     5401 gagcttggag tgacctcacg cgttgctgac cgttggatcc gaatcatttc tatgcgccag
     5461 ctgatcgcta ccgcggcggg ccgacgatga cctgaccgaa atcctcggcc gccttgacca
     5521 tcgcgttacg cgcgggttcg gttggttgcc gcggatcagt aaccaatgtg cccgcctgca
     5581 ggaacatgct ttcgaacccg cccggcgtgc acatcatgag gaggtcggcc tcaggcgagg
     5641 tgatgcggta gccgtgtggg acgttcctcg ggaggaacac gatcccgcct tccgacagct
     5701 ccatctcctc gtcgtcgcac cacaacagcg cagtgccgct gatcagcatg aagatttcgt
     5761 cttcgcgcaa gtgcttgtgg tacgggggtg cttcgccctc gcttacccga aagcggccga
     5821 caccgacttg gccgttcgtc gccgcggcgt cgagcaacac ggcgaacgtt ccgcccgcga
     5881 gccattcaac cgttggctgc tgctcaggtt gcgccaagaa ggcagcgagc atgatgcccc
     5941 t
//
LOCUS       DASWVF010000112         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_207050, whole genome shotgun sequence.
ACCESSION   DASWVF010000112 DASWVF010000000
VERSION     DASWVF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207050"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /gene="carB"
                     /locus_tag="VGD55_01935"
     CDS             <1..1145
                     /gene="carB"
                     /locus_tag="VGD55_01935"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720133.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY0869131.1"
                     /translation="MEIVYGEEMLESYIGRATEASPEHPVLVDRFLDDAVEIDVDALF
                     DGTELYLAGVMEHIEEAGIHSGDSACVLPPITLGSAELRSIRDSTLAIAKGVGVVGLL
                     NVQYALASDVLYVLEANPRASRTVPFVSKATAVPVAKAAARIMTGSTIEQLRAEGLLP
                     PRGDGADLPPNASISVKEAVLPFGRFHGVDTVLGPEMKSTGEVMGIDSDFGTAFAKSQ
                     AGAYGSLPTKGKAFVSVANRDKRAIIFPVKRLADLGFEILATQGTADVLRRNGVTSTV
                     VRKHSDGAGPDGEPTIVQQILAGEVDMIVNTPWGVGPRVDGYEIRTAAVLRGVPCMTT
                     VQGLAACVQGIEALMRGDIGVRSLQEHAAILHADRASYTHTTNSGA"
     gene            1147..>1673
                     /locus_tag="VGD55_01940"
     CDS             1147..>1673
                     /locus_tag="VGD55_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY0869132.1"
                     /translation="MPVQVKGEVLSLRKVGAYYAMTVVAPGVAEVAKPGQFVTFAVGG
                     PDTAMLMRRAFSIHQIHQRGVYGGTVDVVFSVEGRGTAWLARREPHEKLDIVGPLGRP
                     FALPREPATCVLVGGGYGTAPLFPLADALRSRGCRVDFVIGAAKEERLFGALDAKRIS
                     ATLTVTTDDGSVGHPG"
BASE COUNT          272 a          541 c          572 g          288 t
ORIGIN      
        1 gcatggagat cgtttacggc gaggagatgc tcgagtcgta catcggccgc gcgacagaag
       61 cgagtcctga gcatccggtg ctggtcgacc gcttcctcga cgacgcggtc gagatcgacg
      121 tcgacgcctt gttcgacggc accgagcttt acctcgccgg cgtgatggaa cacatcgaag
      181 aagccggcat ccactccgga gactccgcct gcgtgttgcc gccgatcacg ttgggcagcg
      241 cggagcttcg gagcatccga gactcgacgc tggccatcgc gaagggcgtc ggcgtggtcg
      301 ggttgctgaa cgtgcaatac gcgctggcat ccgatgtctt gtacgtgctc gaggcaaacc
      361 cgcgcgcgag ccgtaccgtg ccgttcgtct cgaaggcaac cgcggttccg gtcgccaaag
      421 ctgccgcacg catcatgacg gggagcacga tcgagcagtt gcgggccgag gggctcctgc
      481 cgccgcgcgg tgacggcgcc gacctgccgc cgaacgcctc gatctcggtg aaggaggccg
      541 tgctgccgtt cggccgtttc cacggtgtcg acaccgtcct cggcccggag atgaaatcga
      601 ccggtgaggt catgggcatc gacagcgact tcgggacggc gttcgccaag tcgcaagccg
      661 gcgcatacgg atcgcttccg acgaagggca aagccttcgt gtcggtcgcc aaccgcgaca
      721 agcgggccat catctttccg gtgaagcggc tggccgatct cggctttgaa atcctggcga
      781 ctcaaggcac tgccgatgtg ctgcgccgca acggcgtcac ctcgaccgtg gttcgcaagc
      841 acagcgacgg cgccggccca gacggcgaac ccacgatcgt gcagcagatt ctggcgggcg
      901 aggtcgacat gatcgtgaac accccgtggg gggtgggtcc tcgggtcgac ggatacgaga
      961 tccgtacggc ggcggtgctg cgcggtgtcc cgtgcatgac gaccgtgcaa gggctcgcgg
     1021 cctgcgtcca ggggatcgag gctttgatgc gaggcgacat cggggttcgt tcgctccagg
     1081 agcacgccgc gatcctccat gccgaccgcg ccagctacac ccacacaacc aacagcggag
     1141 cgtgacgtgc ctgttcaggt gaagggcgag gtgctctcgc tgcgcaaggt cggtgcttac
     1201 tacgccatga cggtcgtggc gcctggtgtc gccgaggtcg caaagccggg tcagttcgtg
     1261 acgttcgccg tgggtgggcc tgacacggcc atgctgatgc gccgagcgtt ctcgatccac
     1321 cagatccatc aacgcggtgt ctatggcggc actgtcgacg tcgtgttctc cgtcgagggc
     1381 cgcggcactg catggttggc gcggcgcgaa ccgcacgaga agctcgacat cgtcggccca
     1441 ctcgggcgtc ccttcgcgct tccgcgcgag ccagccacct gcgtgctcgt cggcggcggc
     1501 tacggcacag cgccgctgtt tccactcgcc gatgccctgc gcagtcgtgg ctgtcgggtc
     1561 gatttcgtga tcggcgcggc aaaggaggag cgcctgttcg gcgccctcga cgcgaagcgg
     1621 atttcggcaa ccttgaccgt caccactgac gacggttcgg taggccaccc tgg
//
LOCUS       DASWVF010000113         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_156042, whole genome shotgun sequence.
ACCESSION   DASWVF010000113 DASWVF010000000
VERSION     DASWVF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156042"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGD55_01945"
     CDS             complement(<1..305)
                     /locus_tag="VGD55_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0869133.1"
                     /translation="MDSIAFVKGHGTRNDFVLLPDADGTLELTAELAITLCDRRSGIG
                     ADGVLRVVKSKADPDAVSMAGAATWFMDYRNADGSLSQTCGNGLRVFARYLLDAGLV"
     gene            complement(370..576)
                     /locus_tag="VGD55_01950"
     CDS             complement(370..576)
                     /locus_tag="VGD55_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004392910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY0869134.1"
                     /translation="MREIATRDDVIRLGQFLKLADVVAAGSDAKLLLAEGAVSVNGEP
                     EIRRGRQLVKGDVVTVGPHTLRVG"
     gene            complement(641..>1603)
                     /locus_tag="VGD55_01955"
     CDS             complement(641..>1603)
                     /locus_tag="VGD55_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869135.1"
                     /translation="LVLGPVLILSSAAEMYSLLAYHVDGHDLPLSRLAVVLAPGWALM
                     QVFLPLPILLFPDGRVASRNWRWTLWAYVAVAVTWLSVLLARGSIAFTQHPVRIDSAG
                     MSVHLDGAAHGMSAWIQNVVLVSYVALVVSWIVRLLVSYRRSTGDLRQQLKWLMTGSA
                     LCVVGILSGALLSGIGSALFTLILAFPICIGIAILKYRLYDIDRLISRTLSYLIVTGL
                     LVGLYVGLVALTTHALPLSSPVGVAASTLAVAALFNPVRRRAQRLVDRRFNRARYDAD
                     ATIAAFTLGLRNEIDLESVERDLVETVYRSVQPAHVSVWIRSSA"
BASE COUNT          327 a          512 c          526 g          238 t
ORIGIN      
        1 tcgacgaggc ccgcatcgag caggtagcgc gcaaacacgc gcaggccgtt gccgcaggtc
       61 tgggagaggc taccgtcggc gttgcgatag tccatgaacc aggtcgccgc gccggccatc
      121 gacacggcat cgggatcggc cttggacttc acgacccgca gcacgccgtc ggctccgatg
      181 ccgctgcgcc gatcgcacag ggtgatcgcg agttcagcgg tcaactcgag ggttccgtcg
      241 gcgtcgggca gcagcacgaa gtcgttgcgg gtgccgtgac ccttcacgaa cgcgatgctg
      301 tccacacctg cgatggtacg ggtcgcggcg cgttcgggtc gcctttaacc ggacgccgag
      361 gggccggcgc tacccgacgc gcagcgtgtg cggaccgacc gtgacgacgt cgcccttcac
      421 cagttgccgc ccgcggcgga tctccggttc gccgttgacg ctgaccgctc cttcggcgag
      481 cagcaacttg gcgtccgatc cggcggccac gacatcggcg agcttgagga actgcccgag
      541 cctgatgacg tcgtccctgg tggcaatttc gcgcatggcg tgcatggtca catcatcgcg
      601 cacccctcgg cgtccgcttt gcttagacgc tcgaacttgc ttaggcgctc gaacggatcc
      661 agaccgagac gtgtgcaggc tggaccgagc ggtagaccgt ctcgacgaga tcgcgctcga
      721 cgctctcgag gtcgatctcg ttgcgaagtc ccaaggtgaa cgcggcgatc gtggcgtcgg
      781 cgtcgtaacg agcacggttg aaacggcgat cgacgaggcg ttgcgcgcgc cggcgcaccg
      841 gattgaacaa tgcagcaacg gcaagagtcg acgccgcgac gccgacgggc gatgacagcg
      901 gcagcgcgtg cgtagtgagg gcgaccaagc cgacgtacag gccaaccaac agcccggtga
      961 cgatgaggta cgacagcgtg cggctgatca acctgtcgat gtcgtagagc cggtacttca
     1021 ggatcgcgat tccgatgcaa attgggaatg cgaggatgag ggtgaacaac gccgaaccga
     1081 tcccactcaa caaagcgccc gaaagaattc cgacgacaca aagagcgctg ccggtcatga
     1141 gccatttgag ttgctggcga aggtcgccgg tcgacctgcg gtagctgacc agcaacctca
     1201 cgatccacga cacgacaagt gcaacgtagc tgaccagcac cacgttctgg atccaggcac
     1261 tcatgccgtg tgcggcgcca tccagatgca ccgacatccc tgctgagtcg atgcgcaccg
     1321 ggtgctgcgt gaaggcgatc gaaccacggg caagaagcac ggacagccag gtgacggcga
     1381 ccgccacgta tgcccacaac gtccaccgcc agttgcgcga tgcaacccga ccgtctggaa
     1441 agagcagaat cggcagcggc aagaagacct gcatcaaggc ccacccgggc gccaggacca
     1501 cggcgagacg cgacagcggc agatcatggc cgtcgacgtg atacgccaag agggagtaca
     1561 tttccgcggc gctgctcagg atgaggaccg gaccgagcac gag
//
LOCUS       DASWVF010000114         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_410948, whole genome shotgun sequence.
ACCESSION   DASWVF010000114 DASWVF010000000
VERSION     DASWVF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410948"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1571
                     /gene="clpX"
                     /locus_tag="VGD55_01960"
     CDS             306..1571
                     /gene="clpX"
                     /locus_tag="VGD55_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0869136.1"
                     /translation="MTKSSGSDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREEHKTTLVKSRDGVPGPRDICRVLDDYVIGQSHAKRVLSVAVHNHYKRLAHGA
                     KNNDVELAKSNILLVGPTGSGKTLLAQTLARIIDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQAADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTI
                     ASVPPQGGRKHPQQEFLQVDTTNILFVCGGAFAGLEKIIAQRRQGTSIGFGAEVRGPD
                     ERKTGEILRDLEPEDLLKFGLIPEFVGRLPVVATLDDLDEDALVDILTKPKNALVKQY
                     QRLFEMEDVRLELTDEALRGVAQKAIARKTGARGLRSIMESILLDSMFELPSLAGVSE
                     IVINREVVDGRTKPLHIYSDRRGESGTAD"
     gene            1773..>2047
                     /locus_tag="VGD55_01965"
     CDS             1773..>2047
                     /locus_tag="VGD55_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0869137.1"
                     /translation="MIVPLFVGREKSVRALEDVMKDDKQILLVTQKNAAQDDPTTGDI
                     FMVGTVGTVLQLLKLPDGTVKVLVEGGQRARIVRFAENESFFQAYGEA"
BASE COUNT          401 a          641 c          663 g          342 t
ORIGIN      
        1 agcacacggg acagccgctc gatgcgatcg ttaatgcctt ggagcgtgac aagttcttgt
       61 cgccgatcga ggcgaaggcg ttcggattga tcgacgaagt cgtcgaagcg cgaccgacca
      121 cggccgatga gaaggccagg gcctgagccg cttcgccgcg ggctacgagg atttaaccag
      181 ttgttgatcc atcgggagca cttatactgc aatgaccgag ggagggcgcc gcgagacgcc
      241 cttgcctcgc gccggtacgg cggttccggc cgccttgccc gcgtttggga agcgacggag
      301 cggcgatgac caagtccagc ggcagcgatt caaagaacac gctgtactgc tccttctgcg
      361 gcaagagcca gcacgaggtc aggaaactga tcgccggccc gaccgtgttc atttgcgacg
      421 aatgcgtcga actctgcatg gacatcatcc gtgaagagca caagacgacg ctggtcaagt
      481 cgcgtgacgg cgtgcccggc ccgcgcgaca tctgccgcgt gctcgacgat tacgtgatcg
      541 gccagagcca cgcgaagcgc gtgctctcgg tcgccgtgca caaccactac aagcggctgg
      601 cgcacggcgc gaagaacaac gacgtcgagt tggcgaagtc gaacatcctg ctggtcgggc
      661 cgaccggctc gggcaagacg ctgctggcgc agacgctggc gcggatcatc gacgtgccgt
      721 tcaccatggc cgatgcgacg acgctgaccg aggccggcta tgtcggcgag gatgtcgaga
      781 acatcatcct caagcttctg caggccgcgg attacaatgt cgagcgagcg cagcgcggca
      841 tcgtctatat cgacgaggtc gacaagatct cgcgcaaatc cgacaacccg tcgatcaccc
      901 gcgatgtgtc gggcgagggt gtgcagcagg ctctcctcaa gatcatggag ggtacgatcg
      961 cctcggtgcc gccgcagggc ggccgcaagc atccgcagca ggaatttctc caggtcgaca
     1021 cgaccaatat cctgtttgtc tgcggcggtg cgttcgcggg gctggaaaag atcatcgcgc
     1081 agcgtcgcca gggcacctcg atcgggtttg gcgccgaggt gcgcggcccc gacgagcgca
     1141 agaccggcga gatcctgcgc gatctcgaac ctgaggatct gctgaaattc gggctgatcc
     1201 cggaatttgt cggccgcttg ccggtggtag cgacgctcga cgacctcgac gaggacgcgc
     1261 tggtcgacat tctgaccaag cccaagaacg cgctggtcaa gcagtaccag cggctgttcg
     1321 agatggagga tgtccggctc gaactgaccg acgaggcgct gcgcggggtc gcccagaagg
     1381 cgatcgcccg caagaccggt gcgcgcggcc tgcgctcgat catggagtcg atcttgctcg
     1441 attcgatgtt cgagctgccg agccttgccg gcgtcagcga aatcgtgatc aaccgtgagg
     1501 tcgtcgacgg gcggacaaaa ccgctccata tctattccga ccgccgcggc gaaagtggga
     1561 ccgccgattg acctgtcccg cgcggtgcgg gatgcgccat gatgcggccc cggcttgacg
     1621 accggcgacc aaaggccaaa ctaatcacgg cctagctcga ccgcccacag gtggtggacg
     1681 gtccccgacc gaaaagaggg cctatgtcag aacctgcacg cgttgcgctc taccccgtcc
     1741 tgccgctccg cgacattgtc gtcttcccgc atatgatcgt gccgctgttt gtcggccgcg
     1801 agaagtcggt gcgcgcgctc gaagacgtga tgaaggacga caagcagatc ctgctcgtca
     1861 cccagaagaa cgccgcgcaa gacgatccca ccaccggcga cattttcatg gtcggcacgg
     1921 tcggcaccgt cctccaactg ctgaaactgc ccgacggcac ggtcaaggtg ctggtcgagg
     1981 gcggccaacg cgcgcgcatc gttcgctttg ccgagaacga gtcattcttc caggcctatg
     2041 gcgaggc
//
LOCUS       DASWVF010000115         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_54157, whole genome shotgun sequence.
ACCESSION   DASWVF010000115 DASWVF010000000
VERSION     DASWVF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54157"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VGD55_01970"
     CDS             complement(<1..1357)
                     /locus_tag="VGD55_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869138.1"
                     /translation="MHRRLIAASLSAAAAALFLGVSGTALASPTVVRYAGADRYATSA
                     AMVDANYAPGVPVAYIATGLNFPDALAGGAAAANQGGPLLLTDPNAIPDAVGAELRRL
                     TPARIVVLGGLSSVSASVATALAAYTTGTVTRVSGTDRYATAALLAGTFPSGSPVYVA
                     TGLDFPDALAATAAAAAQHAAILLTDPSTLVGATAQALAALHPASITIVGSTTAVSAA
                     VQTQLGTYSTEVRRLSGSDRYSTAAAIAAVEFPSATGVFLASGAGFADALTGGPVAGS
                     HDQPLLLASDTCLPTPTSNEITALGVTTETLLGGPATLSSGVAALTSCPASAAPSAPS
                     APESPPSTPSSPPKTGSVPVYNFGANVNPSSYPGASLTPDTPDGDGWPARECTAYVVW
                     WLDQHDVEIHASTLIGPAGSHTLGNAGGWDAAAKAVGWAITATPVVGAVAQWHDNEST
                     SD"
     gene            complement(1342..1629)
                     /locus_tag="VGD55_01975"
     CDS             complement(1342..1629)
                     /locus_tag="VGD55_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869139.1"
                     /translation="MIAVYQFIGAVAIVVSLVGTWLAPRHRAGWLICVASSTMWFPAL
                     ATGNQWAAVSNCCLSIAICVRNFWVSGQRLGVVAGADCNAADYRVSSCIAD"
     gene            complement(1717..2541)
                     /locus_tag="VGD55_01980"
     CDS             complement(1717..2541)
                     /locus_tag="VGD55_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869140.1"
                     /translation="MSVATGQVDLALVKARQQKTWASGDFSVVASRIVLVSELLAESA
                     DLRAGWQVLDVACGNGNATLAAARSGAHVYGVDYVEALLEDGRGRALAEGLDVEFRLG
                     DAEDLPLESGSVDAVLSVFGTMFAPNHQRTADEVIRVARPGGTVALASWTPDGFIGQM
                     FRVITAHVPAPAGVRSPLLWGTEEHLVELFGSALSSVHSQERECVWRFTSPEEFVAFF
                     RRWYGPTLKAFEALDDIGRTALAADLTDLARKFDRNSDGGSIAIPATYLESVITLR"
     gene            2760..3467
                     /locus_tag="VGD55_01985"
     CDS             2760..3467
                     /locus_tag="VGD55_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0869141.1"
                     /translation="MTSYADYCPIAIGVDVLGDRWTPLVIRELMVGASGFNDIHRGIP
                     RISRTLLAQRLRQLERGGLVNRDVSERGRAGRYVLTPAGEALTPIVWAMGHWAAEWVF
                     GDPTEEDCDGLSVIWRLHQHAVPTKLPATRTVVHLVLTGHGGAQGWLALDSAGATVCR
                     DDQGLSVDLAVEADTAQMHRWLVGRATFRDLVSKGHARLLGPSRLARAFPTWFDMTIF
                     AEDLRRAASREEAVAIA"
     gene            complement(3506..>3795)
                     /locus_tag="VGD55_01990"
     CDS             complement(3506..>3795)
                     /locus_tag="VGD55_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869142.1"
                     /translation="EAGLGAGLLNTSQQLGGAIGVAIASTVAASRYKTLLHNGSASSV
                     ALTGGFQSAFWVCAAVGLAAVPVTFLLIRRGELAKAVARSVAPRPILAPAD"
BASE COUNT          685 a         1257 c         1249 g          604 t
ORIGIN      
        1 aatcagacgt tgattcgttg tcgtgccact gcgcaaccgc accgacgacc ggagttgcgg
       61 tgatggccca gccgactgct ttcgccgccg catcccaccc gcccgcgttg ccaagtgtgt
      121 gcgacccagc cgggccgatc agcgtcgaag cgtgaatctc gacatcatgt tggtcgagcc
      181 accacacgac gtaggcggtg cactcgcgag ccggccagcc gtcgccatcg ggggtatcag
      241 gcgtgagact ggcgcctggg taactgctcg ggttgacgtt ggcaccgaag ttatagacag
      301 gaactgaccc tgtcttcgga gggctacttg gtgtgctggg aggactttcg ggtgcactcg
      361 gagcgctagg cgctgcgctc gccggacagc tggtgagggc ggccacgcca ctcgataacg
      421 tcgccgggcc tcccaacagc gtttcggtgg tcacgcccag cgcggtgatc tcgttcgacg
      481 tcggagtcgg caaacaggtg tcagacgcga gcagcagtgg ctggtcatgg cttccggcga
      541 ccggcccgcc cgtcagcgca tcagcaaagc cggcgccgga cgccaagaac acgccggttg
      601 cggaggggaa ctcgaccgcg gcaatcgccg ctgcggtcga ataccggtcg ctgccgctga
      661 ggcggcgaac ctcggtgctg taggtgccca actgggtctg caccgctgcc gagacagccg
      721 tcgtcgatcc gacgatggtg atgctggccg gatgcaacgc ggccagcgct tgggcggtcg
      781 ccccgaccag agttgacgga tcggtcagca ggatcgcggc atgttgagcg gccgcggctg
      841 ccgtggcggc gagggcatca ggaaagtcca acccagtcgc cacgtacacc gggctgccgc
      901 tgggaaaagt gccggcgagg agcgccgcgg tagcgtaccg atcagtgccg gaaacccgag
      961 taaccgtccc ggtcgtgtac gcagccagtg ccgttgcgac tgacgcgctg accgaagaca
     1021 agccgccgag caccacgatg cgagcagggg tgagccgccg cagttcggcg ccaacagcat
     1081 ccggaatggc gttcgggtcc gtcaacagca gcgggccacc ttggttcgcg gccgctgccc
     1141 ccccggcgag cgcatcgggg aagttcaaac cggtcgcgat gtaggcgacc ggaaccccgg
     1201 gcgcgtagtt cgcgtccacc atcgccgcgc tggtcgcata cctgtccgcc ccggcatagc
     1261 ggacgacggt tgggctggcg agtgccgtgc cggacacccc taggaacagg gctgctgcag
     1321 cggcactcag gcttgcggcg atcagtcggc gatgcatgag ctcacacggt agtcggccgc
     1381 gttgcagtcg gcacctgcga ctacgcctaa tctctgcccc gaaacccaga aatttcgcac
     1441 gcagatggcg atcgataggc aacagttcga cacggctgcc cactggttgc cggtcgcgag
     1501 cgcgggaaac cacatcgtcg acgacgcgac gcaaatcagc cagcccgcgc gatgtctggg
     1561 tgcaagccag gttccgacaa ggctgacgac gatggcgacg gcaccgatga actgatagac
     1621 ggcaatcacg agaaaattcc ttgaaatagg ggccatcgct gaaggtccgc acccgagtcg
     1681 atgacgtagg cgcggacctt caagctgacg gttgattcag cggagggtga tgacggactc
     1741 gaggtaggtc gcagggatcg cgatgcttcc gccgtcgctg ttccggtcga actttcgcgc
     1801 aagatcggtg aggtcggccg cgagcgccgt ccgaccaatg tcatcgagcg cctcgaatgc
     1861 cttcagcgtc gggccgtacc aacggcggaa gaacgcgacg aactcctcgg gtgaggtgaa
     1921 gcgccacacg cactcgcgtt cctgcgaatg gacgctcgac aacgccgagc cgaacagttc
     1981 gaccagatgt tcctcggtgc cccacagcaa aggcgaacgg acgccggccg gtgcgggcac
     2041 gtgtgccgtg atgacgcgga acatctgccc gatgaagccg tcgggagtcc acgatgcgag
     2101 cgcgaccgtt ccccctggcc tggcgacgcg gatgacctcg tccgctgtgc gttgatggtt
     2161 gggcgcaaac atcgttccga acacactcag cactgcgtcg accgacccgc tttcgagcgg
     2221 cagatcctcg gcatcgccca gccggaactc gacatccagg ccctcggcga gggcacgccc
     2281 ccgaccgtcc tccaagagcg cctcgacgta gtcgacgccg tacacgtgcg cccccgaccg
     2341 cgccgcagcc agtgtcgcgt tgccgttgcc gcaggcaacg tcgagcacct gccacccagc
     2401 gcgcaggtca gcgctttccg ccagcaactc actcaccagc acgatgcgcg acgcgaccac
     2461 cgagaaatca ccactcgccc acgtcttctg ctgtcgtgcc ttgaccagag ccagatcaac
     2521 ctggccggtt gcgactgaca tttctcctcc tcgttagcga cgacccaggt ggtcgtcttg
     2581 ctagccagga tctgggtgaa cagcacctcc gaacgagtga cggtctcgta ccaccgagac
     2641 atcgtgggtt ctgagcgcgc cgagccggac ggggtgaacg atgacacgct cgcccgagcg
     2701 gtgattggag tacaggattt gtactaccct ccgaggctcc cgcagggcag gattacccca
     2761 tgacgagtta tgccgactac tgccccatcg ccatcggagt cgatgtcctg ggcgaccggt
     2821 ggacgccgct ggtgatccgc gagctcatgg tgggcgcctc cggattcaat gacatccatc
     2881 gtggtattcc acgcatcagt cgcaccctgc tggcacaacg cctccgccag ttggagcgag
     2941 gcgggttagt caatcgcgat gtgagcgagc gagggcgagc cggccgctat gtgctcaccc
     3001 cggctgggga ggcgctgacc ccgattgtct gggctatggg ccactgggcg gcggagtggg
     3061 ttttcggcga tccgaccgag gaagactgtg acgggctctc ggtcatctgg cgcctgcacc
     3121 aacacgccgt cccgacaaag ctgcccgcaa cgcgcacggt ggtgcacctc gtgctcaccg
     3181 gccacggcgg agcgcaaggt tggctcgcgc tcgacagtgc aggcgccacc gtctgtcgcg
     3241 acgatcaggg cctcagcgtc gacctcgcgg tcgaagccga caccgcgcag atgcatcgct
     3301 ggctcgtcgg gcgggcaacg tttcgcgact tggtcagcaa gggacacgcg cgtctgctgg
     3361 gtcccagcag acttgcgcgc gcgttcccca catggttcga catgacgatc tttgctgagg
     3421 atctacgtcg cgcggcgtca cgcgaagagg cggtcgccat cgcctgacgg cgcgccgcgg
     3481 ttgcgccggc cgcgacgccg ccatctcagt cggccggcgc aagtatcggt cggggcgcga
     3541 ccgagcgagc gacagccttg gcgagttcgc ccctgcggat cagcaggaac gtcaccggga
     3601 cggcggcgag cccgaccgcg gcgcataccc aaaacgcaga ttggaatccc ccggtcagag
     3661 ccactgacga cgcgctgccg ttgtggagca acgtcttgta gcggcttgct gccacggtcg
     3721 aggcgatcgc cacgccaatt gctccgccaa gctgctgcga cgtattcaac agacccgcac
     3781 cgagcccggc ttcat
//
LOCUS       DASWVF010000116         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_462001, whole genome shotgun sequence.
ACCESSION   DASWVF010000116 DASWVF010000000
VERSION     DASWVF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462001"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGD55_01995"
     CDS             complement(<1..561)
                     /locus_tag="VGD55_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0869143.1"
                     /translation="MRRLFLAFLARFGLQETSLRFRAVWGAMWAVGGLGATQVIRFAS
                     NLVLTRLLFPEAFGLMSLVQVFLAGLHMFSDVGVTPAIIQSNRGNDPRFLNTAWTIAV
                     VRGCLLWVAACILAYPASIFYREPMLLRLMPAVGFTAALSGLNSTKLATANRELSLKR
                     ITIIDLSSYVASMIVTIVLSWLWRSVW"
     gene            complement(579..>1903)
                     /locus_tag="VGD55_02000"
     CDS             complement(579..>1903)
                     /locus_tag="VGD55_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY0869144.1"
                     /translation="GEVILSYATTDGVPSTMQRRAAGWINDLSRKQLESLLTTTVPHF
                     QHVGAWETQEIYRLSSQLLVPLPDDARLGPRRRVTLCGSFGFGNAGDEGAWMATEDLA
                     HALGFEVAFDLVTRFPKPFMKRVIGTGPEEHARRQRLRDQKVVFVGGGVIDASQFCVL
                     LANEPFLRETSARSFGLLGVNVETGKRYDPVVTAGLRALLSEANQVTVRDQLSAETLR
                     AIAPEVKSQVVGDVVLWLTPTKLGLPAEVAALPRYVAVNAPRWKDDRAWRAWITDELI
                     KVSRALDAPLVFVPCSTHFDDDRPAHSELASDIRRRDPGVTVVEVKQHLDPRQTAGLF
                     AGAVLTVGMRLHACVMSYGNRVPCVGLAYHSKLHGFFRTVGAEAFLLPGGLPTAPHEV
                     SSGFSFADSGLGGSDLASAAREAIARFPFDRLAALKDASAEAFRRIVA"
BASE COUNT          383 a          632 c          607 g          281 t
ORIGIN      
        1 ccagacggaa cgccagagcc acgacaagac gatggtcaca atcatcgatg ccacgtagga
       61 cgacagatcg atgatcgtga tccgcttcag cgacagctca cggttggccg tggccagctt
      121 cgtcgagttg agcccggaaa gcgccgccgt gaagccgacc gcaggcatca gccgaagcag
      181 catgggctcg cgatagaaga tcgacgcggg ataggccagg atgcacgcgg cgacccacag
      241 caggcagcct cgtacgacgg cgattgtcca cgccgtattc aggaagcggg ggtcgttccc
      301 gcggttgctc tgaatgatcg cgggagtaac tcccacgtcg gagaacatgt gaagcccggc
      361 caggaacacc tggacgagcg acatgagccc gaaggcctcc ggaaagagca gtcgcgtgag
      421 gaccaggttg ctggcaaagc gaatgacttg ggtcgcccca agccccccga cagcccacat
      481 cgcaccccag acggcgcgaa accgaagcga cgtttcttgt aacccgaatc gcgcgaggaa
      541 cgctaagaac agccgccgca tctatgccga ttcgttcttc aggccacgat acgtcggaac
      601 gcctccgcgc tcgcgtcctt gagcgcggcg aggcggtcaa atgggaagcg cgcaatcgcc
      661 tctcgcgcgg cggacgctag atcgctgccg ccgaggccac tatcagcaaa cgaaaacccg
      721 gagctcacct cgtgaggtgc agtcggcagc cctcccggta gcaggaaagc ctccgccccg
      781 acggtccgga agaagccatg cagcttcgag tggtacgcga gaccgacgca cgggacgcgg
      841 ttgccgtacg acatgacgca ggcatgaagg cgcatgccga cggtgagcac tgcgcccgcg
      901 aaaagaccag cggtctgccg gggatccagg tgctgcttga cctccacgac cgtgacgccc
      961 gggtcgcgac ggcgaatatc actcgccagt tcgctgtggg cgggccgatc gtcgtcgaag
     1021 tgagtcgagc agggaacgaa cacgagaggt gcgtcgaggg cccggctcac cttgatcagt
     1081 tcgtcggtga tccaggcgcg ccaggcacga tcgtccttcc accggggggc gttgaccgcc
     1141 acgtaccggg gaagcgcggc cacctccgcg ggcagaccga gcttcgtcgg agtcaaccac
     1201 aacacgacgt ctcccacgac ctgcgacttc acctccggcg caatcgcgcg cagtgtctcg
     1261 gcggagagct ggtcgcgcac ggtcacctgg ttggcctccg acagcagcgc tcgcaacccg
     1321 gcagtcacga ccgggtcgta gcgtttgccc gtctcgacat tcacgccgag cagaccgaag
     1381 gaccgcgccg acgtttcgcg caagaacggt tcgttcgcga gcaggacaca gaactgcgac
     1441 gcgtcgatga cgcctccgcc gacgaaaacg accttttgat cgcggaggcg ttgcctgcgt
     1501 gcatgctctt cggggccggt tccaatcacc cgcttcatga acggcttcgg gaaccgtgtc
     1561 accagatcga acgccacctc gaacccgagc gcgtgcgcca ggtcctccgt tgccatccac
     1621 gcgccctcgt cgcctgcatt accgaacccg aacgagccgc acagggtgac tctccgtcga
     1681 ggaccgagcc gtgcgtcgtc gggaagcggg acgagcaact gcgacgacag gcggtagatc
     1741 tcctgggtct cccacgcacc gacgtgctgg aagtgaggga ccgtcgtcgt cagcaatgat
     1801 tcgagctgct tccggctcag gtcgttgatc cagccagcgg cgcgccgctg catggtcgac
     1861 ggaacgccgt ccgtcgtcgc gtacgagagg atcacctcgc cgc
//
LOCUS       DASWVF010000117         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_130806, whole genome shotgun sequence.
ACCESSION   DASWVF010000117 DASWVF010000000
VERSION     DASWVF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130806"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VGD55_02005"
     CDS             <1..1118
                     /locus_tag="VGD55_02005"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEY0869145.1"
                     /translation="AAGVSQAELAVRSLGTAITADGLDLTVDAHTGVACAPSDGDNFD
                     DLIRRARLAMYQSRADDVPVYRYQPELEPPALAQLELLRDLREALESKQLVLHYQPKF
                     SMRGAMPVAAEALVRWNHPERGLIPPAEFIPLLERVGLVGDLTRYVLEAAIEECAAWN
                     SMGLELSIAVNLSARNLLDEDLPRFVFECLARNGLPAERLICEITETAVFSRSPIAAS
                     ILDQLRGAGISLSLDDFCTGYSSLSLLRERAIDEIKIDRSFVADLGIGGRTTHIVTAL
                     IELAHRCDIVVTAEGIETAEQQQLLTTLGCDHGQGFLLARPMTAASVRAFFLEAMRTA
                     PARPVRRSSVTTVKALRSTRTSPDWDDPLPWREAASE"
     gene            1410..2702
                     /locus_tag="VGD55_02010"
     CDS             1410..2702
                     /locus_tag="VGD55_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0869146.1"
                     /translation="MGVGVFPWGEAMDAVDVVEDEVRELVRRRGIDPTVDGAGVRLLV
                     EQVVADYDERTLSSSLPPLADPRGAIRAVYDAVAGFGPLQRYLDDPAVEEIWINEPGR
                     VFIAKRGRSELTNTILSSAQVRDLCEKMLKSSGRRVDLSTPFVDAMLPDGSRLHVVIP
                     DITRCHWAINIRKFVLTAHSLDELVALGSITVQAARFLEAAVASGLNIIVAGGTQAGK
                     TTLLNCLTAAIPAHERVVTCEEVFELRVPLPDVVSMQTRQANLEGTGEVKLRRLVKEA
                     LRMRPSRIIVGEVRQEECLDLLIALNSGLPGMCTIHANSAREAVTKMCTLPLLAGENV
                     GHAFVVPTVASSVDLVVHTRSDNRGHRRVGEIVAVPGRVEGAGDGSVIEIEDVFTSRK
                     NRLVRADGFPPHAERFEAAGFDLLELLAQERYDAVRAG"
     gene            2817..3566
                     /locus_tag="VGD55_02015"
     CDS             2817..3566
                     /locus_tag="VGD55_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0869147.1"
                     /translation="MSEVLAQAGIESVTPNQLLASSIGLALAVFLFTAAISRAFTIAV
                     AFALFAALGPISLVRHRQRKRRTELRDLWPEAVDNLASAVRAGLSLPEALTQLGSRGP
                     AELRRPFQRFGEDYRATGRFSQCLDRLKAALADPTGDRIVESLRLARDVGGTDLGRLL
                     RTLSAFLREDARTRAELETRQGWSVNGARIAGAAPWLLLAALSLHPEAVRAYNSTAGA
                     LVIAVGGALSLLAYRVMIRIGRLPVEERVLR"
     gene            3563..4498
                     /locus_tag="VGD55_02020"
     CDS             3563..4498
                     /locus_tag="VGD55_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0869148.1"
                     /translation="MSVLVGVALGGVAGAGATFGVYSVPRYRRPGLDDRLAPYLRDAP
                     RRSSLLSQDPMRTPFPTLERILRPVMADATRLLDRLLGGVASTRRRLEQAGRRMTVDE
                     FRAEQVLWGAVGLLGGVGMSLLLLADNPHRPPLLLVLLCIAAAISGVVARDFALTQEV
                     SRRERRILAEFPTVAEMLALAVGAGEGAVSALERVTRTSDGELAKELGVALAEARAGT
                     SLIGALENVAARTSLPILARFVDGIAVAVERGTPLAEVLRAQAVDVREAGKRALLESG
                     GKKEVAMMVPVVFFILPVTMLFALFPGFVSLRLLA"
BASE COUNT          746 a         1391 c         1477 g          898 t
ORIGIN      
        1 cggccgccgg agtgtcgcag gctgaactcg ctgtccgatc gcttggaact gcgatcaccg
       61 ccgacggtct cgacctgacc gtggacgcgc acaccggcgt cgcatgtgcg ccgtcggatg
      121 gggacaattt cgacgatctc atccgccgcg cacggctcgc gatgtatcag tcgcgcgccg
      181 acgacgtccc ggtataccgg taccagccgg aactcgaacc acccgcattg gcgcagcttg
      241 aactcctgcg tgatctacgc gaggcgcttg aatcgaagca gcttgtcttg cactaccaac
      301 cgaagttcag catgcgcggt gcgatgcccg ttgcggcaga agcgctggtg cgatggaacc
      361 atcccgagcg cggcctgata ccgcccgccg aattcattcc gttgctcgaa cgtgttggcc
      421 tggtaggcga cctcacccgc tatgtgttgg aagctgcgat cgaagaatgc gcggcctgga
      481 attcgatggg cctggagttg tcgattgccg tgaacctgtc ggcgcgcaat ctgctcgacg
      541 aagacctacc tcggttcgta ttcgaatgcc tggcgcgcaa cggtctgccg gccgagcggc
      601 tcatctgcga gatcaccgag acagcggtat tctcgcgaag cccgatcgca gcatcgattc
      661 tcgaccagct tcgtggcgcg ggcattagtc tctcgctcga cgacttctgc acggggtact
      721 cgtcgctttc cctgctgcgt gaacgggcca ttgacgagat caagatcgac cgatccttcg
      781 ttgccgatct gggtatcggt ggccggacga cgcacatcgt cactgcgctg atcgaacttg
      841 cgcaccgttg cgacatcgtc gtaacggcgg agggcatcga gaccgcggag cagcagcagt
      901 tgctgacgac gctcggctgc gaccacggcc agggcttcct gcttgccagg ccgatgacgg
      961 cagcctcagt acgagccttc ttcctcgaag cgatgcggac ggcgccggcg cgcccggtca
     1021 gaaggtcttc cgtgacgacg gtcaaagcat tgcgatcaac gcggacgtcg ccggactggg
     1081 acgacccgct gccctggcgg gaagcggctt cagagtaggc acaagatcac cacgctccgc
     1141 cttccgatcc tctaagacgc acgctcgtgg tcgccgacgc aatccatcga cacccgaccc
     1201 caccattaat gcctctttga gttcgtatag caccttaatg ccgtttatat gacgaacgag
     1261 tttatgcacc gcttcgggtc gggagtgggc cgttcggact gacttcggga cttcgccacg
     1321 ctcaggaatt cagatctatc cacagggtcg agcgaatggt gcgaatcgct gcccaattgc
     1381 agctactgtc caagactgtt gggccggctg tgggcgtcgg tgtattcccg tggggggaag
     1441 ccatggacgc cgtcgacgtt gtcgaggacg aagtcaggga gctagttcga cggcggggga
     1501 tcgatcccac cgttgacggt gctggcgtgc gcctgctggt cgagcaggtt gttgccgact
     1561 atgacgagcg cacgttgagc agctcgttgc cgccgttggc cgacccgcga ggcgcgattc
     1621 gggccgtgta cgacgccgtc gcagggttcg gtccgctgca gcgctacctc gacgatccag
     1681 ctgtcgagga gatctggatc aacgaaccag gtcgggtctt catcgctaag cgcggccgca
     1741 gcgagcttac gaacacgatc ctgagcagtg cgcaggtgcg tgatctatgc gagaaaatgt
     1801 tgaagagttc gggacgtcga gtcgatcttt cgacgccgtt cgttgacgcc atgctccccg
     1861 acggaagccg gctccatgtc gtgattcccg acatcacgcg gtgccactgg gcgatcaaca
     1921 ttcgcaagtt tgtcttgacc gcgcactcac tcgacgaact cgtagccctc ggttccatca
     1981 ccgtccaggc ggcacggttt cttgaggccg ccgttgccag cgggctgaac atcatcgttg
     2041 ccggcggaac gcaggctggc aaaaccactc tcttgaactg tcttaccgcg gcgattccgg
     2101 cacacgaacg tgtcgtcacc tgcgaagaag tcttcgaact tcgggtgccg ctgcctgacg
     2161 tcgtctcgat gcagacccgg caggcgaatc tcgaaggcac cggcgaggtc aagcttcggc
     2221 ggctcgtgaa ggaagccctg cgcatgcgcc cctcgcgaat cattgttggc gaggtgcggc
     2281 aagaggaatg tctcgacctt ctcatcgccc tcaactctgg gctgccgggc atgtgcacta
     2341 tccacgccaa cagtgcccgc gaagctgtga cgaagatgtg tacgttgcct ttgctcgccg
     2401 gagagaacgt cggccacgcg ttcgtggtac ctaccgttgc aagcagtgtt gacctcgttg
     2461 tacacacgcg atccgacaat cgcgggcatc gacgggtcgg tgagatagtc gccgtgccgg
     2521 gtcgggtcga gggtgccggt gacggcagtg ttatcgagat cgaggacgtg ttcacgagtc
     2581 gcaagaaccg gcttgtgagg gcggacggct ttccccctca cgctgagcgt ttcgaggcag
     2641 ccggcttcga cctacttgag ctactggctc aggaacgcta cgacgccgtt cgggccggct
     2701 gacgcatggg cgcttttctc gggttgctct gcggagtcgg actggcgctg gtttggcgtt
     2761 cgggttcgcg ccgtccgccc aagcgttctc gtagccggcc gtcgggcaag gagcgcgtga
     2821 gcgaggtcct tgcccaggcc ggaatcgagt cggtgacccc gaatcagttg ctcgcgtcgt
     2881 ccatcggtct ggctcttgct gtgttcttgt tcacggccgc gatctcacgc gcgtttacca
     2941 tcgcagtcgc gtttgcgctc tttgccgcgc tcggaccgat ctcgctcgtg cggcaccgac
     3001 aacgcaagag acggaccgag ctacgtgatc tgtggccaga ggccgtcgac aacctcgcat
     3061 ctgcagtgcg cgcagggctt tcgctgccgg aggcgctcac gcaactggga tcacgcgggc
     3121 cagcagaact gcggcggccg ttccagcggt ttggcgagga ctaccgcgcg actggccggt
     3181 tctcgcaatg cctcgatcga ctcaaggcgg ccctcgccga tcccacgggg gatcgcatcg
     3241 tcgaatcgct gaggttggca cgcgatgttg ggggaacaga cctgggccgc ctgctccgca
     3301 ccttgtcggc atttcttcgt gaggatgccc gcacccgtgc cgaactcgag acacgccagg
     3361 gatggtcagt caacggcgcg cgtatcgcgg gggctgcacc gtggttgctg ctagcagcgt
     3421 tgtctttgca tccggaggcg gtgcgtgcgt acaactcgac tgctggcgcg ctcgtcatcg
     3481 cggtcggtgg cgcgctgtcc ttgctggcgt atcgggtgat gatcagaatc ggtcggctcc
     3541 ccgtcgagga acgggttttg cgatgagcgt cctggtcggc gtcgcactcg gcggcgtcgc
     3601 cggagctggt gcaaccttcg gggtctatag cgtgccgcgc tatcgacgtc cggggcttga
     3661 cgaccgcctc gcgccgtacc tgcgcgatgc gccgcgacgg tcgagcttgt tatcacaaga
     3721 cccgatgcgg acgccgttcc cgacccttga gcgcatcctg cggccggtca tggctgacgc
     3781 aacgcgactg ctcgatcggc tactgggcgg cgttgcgtcg acgcgacgtc ggctcgagca
     3841 ggcgggtcgt cgcatgacgg tagacgagtt tcgcgcggag caggtcttgt ggggcgcagt
     3901 cggcctgctg ggaggcgtcg gcatgagcct gctcctgctc gcagacaatc cgcaccggcc
     3961 gccgttgctc cttgtcctgc tgtgcatcgc ggctgcgata tcaggggtcg tggcgagaga
     4021 ctttgcactg acccaggagg tgtcgcgacg cgaacgccgg atccttgcgg agtttccaac
     4081 ggtcgccgaa atgcttgccc tggcagtggg cgctggcgag ggtgcagtga gtgcactcga
     4141 gagggtgacc cgcacgagcg atggtgagtt ggccaaagaa ctcggagttg cccttgccga
     4201 ggcgcgcgcc ggcacatccc tgatcggggc actggagaac gtcgcggcgc gcacgtcgct
     4261 gccgatactc gcgcgcttcg tcgatggcat cgccgttgca gtcgagcgtg gtacgccgtt
     4321 ggccgaggtg ttgcgtgccc aggccgtcga cgtccgtgag gccggcaagc gcgcactgct
     4381 tgaaagcggc ggcaagaagg aagtcgcgat gatggttcca gttgtcttct tcatactgcc
     4441 ggtcaccatg cttttcgcgt tgtttccggg atttgtctca ctgcggctac tcgcatgagc
     4501 cagcgatgaa at
//
LOCUS       DASWVF010000118         9632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_79591, whole genome shotgun sequence.
ACCESSION   DASWVF010000118 DASWVF010000000
VERSION     DASWVF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79591"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VGD55_02025"
     CDS             <1..520
                     /locus_tag="VGD55_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869149.1"
                     /translation="GAIFVVGGFFSFTGSTGKSAVDHIATVLGTLGLAALPVFVGIAI
                     LKYRLFEIDRLISRTLSYAIITGLLVGVYVGLIALTTRALPLSSPIGVAASTLAAAAL
                     FTPLRRWVQRIVDRRFNRARYDAEATVAAFSAGLRDAVDLDRVQRDLIGVVNLSMQPS
                     HVSVWLREQSPV"
     gene            737..1228
                     /locus_tag="VGD55_02030"
     CDS             737..1228
                     /locus_tag="VGD55_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEY0869150.1"
                     /translation="MSTHGVLYVHSATPAVTPHVEWAVAGVLSTPVRFDWTSQPVSPG
                     SLRAEVTWMGETGCGGRLASALLGWPVRFEVTEDPSPGCDGERYAFTPGLGLFHAMTS
                     ANGDLVVPENRLRQLLTANLVQVADVASDLRTLLGEPWDAELEPFRHAGEGTPVRWLH
                     QVV"
     gene            complement(1279..2679)
                     /locus_tag="VGD55_02035"
     CDS             complement(1279..2679)
                     /locus_tag="VGD55_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0869151.1"
                     /translation="MTTTTADTALDPRSPRARLTALFDDDSFEPMTADDDSGVLAGIG
                     RMLGTPAIAFACDPTIQGGAMGSAGCTAIVEAYERALADGVPIVGLWHSGGARLREGV
                     ESLDGVGRVFAAMTRASGKVPQISVVLGPAAGGAAYGPALTDIVIMGPAGRIFVTGPD
                     VVRSVTGEDVDMERLGGAEVHGRRSGVVHIVTESEEDALERARVLTELLGNQGKIGDA
                     DLVDIDLESLLPEGAKRAYDVHPLINGLLDSPGVELHAKWAPNIVTVFGRLAGRTVGV
                     VANNPLRLGGCLDATSAEKAARFVRTCDAFGVPLVVVVDVPGYLPGLGQEWDGVVRRG
                     AKLLHAFAEATVPRVTLITRKAYGGAYIAMNSRSLGATAVLAWPGAEVAVMGSVAAVR
                     ILHRRKLAEVGEALRPSLELELAAEHEKLAGSIDKAVAIGVVDSVVSPASTRSMIADA
                     IAAAPPRRGSHGNIPL"
     gene            complement(2702..3931)
                     /gene="fabF"
                     /locus_tag="VGD55_02040"
     CDS             complement(2702..3931)
                     /gene="fabF"
                     /locus_tag="VGD55_02040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0869152.1"
                     /translation="MNARQVVITGLGATTPIGGDVASTWAALLAGKSGVGALTEEWAA
                     DLPVRIAARVEVEPLDVLDRVEARTLDRSQQFAIIAAREAWAQAGKPDVEKERLGVVF
                     ASGIGGVTTLLNQYDVLREKGPRRVSPHTVPMLMPNGPAAAVGLDLQARAGVHTPVSA
                     CASGAEAIAYGLEMIRTGRADVVVCGGTEAAIHPLPLAAFAAMRAMSTRNDEPLRASR
                     PYDKGRDGFIMGEGAGAVVLESAEHARARGATILGEIAGAGITSDGYHIAAPDPEGRG
                     VVRALRAALENAGLAPSDITHINAHATSTPAGDVAEAKAIRQVFGSAADSVVVTAPKS
                     TFGHLLGAAGAVEAIATLLSINEHIVPPTLNLDDRDDDVELDVVTVDPRPLRPGPAMS
                     NAFGFGGHNVVLVIKPA"
     gene            complement(3955..4209)
                     /locus_tag="VGD55_02045"
     CDS             complement(3955..4209)
                     /locus_tag="VGD55_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0869153.1"
                     /translation="MALTQQEILAGLAEIVNEVAGIPASDVQMDKSFTDDLDVDSLSM
                     VEVVVAAEEKFGVRIPDDDVKNLKTVGDAVTYIEKAGVTA"
     gene            complement(4260..5273)
                     /locus_tag="VGD55_02050"
     CDS             complement(4260..5273)
                     /locus_tag="VGD55_02050"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0869154.1"
                     /translation="MKTLTRQAGSPGARIVAIGEHRPTRIVTNEEICRDIDSTDAWIR
                     ERSGIITRRFAAPDETVVDMATSAASKALASSGIAATDVELVLLATCTHPYQTPGGSS
                     EVQDRLGAVNAGAMDINAACAGFAYALSVANDAIRAGSARNVVVIGSEKMSEFIDPHD
                     RGMAFLFGDGAGAAVVSASQTTGIGPVVWGSDGSHALTITQEPNYTQVQHALATGESL
                     PPVALKMAGQTVFRWAVTQMAPVARRALEAAGVAPEELGAFVPHQANLRIVESLVKSL
                     KLPDTVAIARDMETQGNTSSASIPLALSALLERGEVESGQPALLIGFGAGLTYAAQVV
                     EIP"
     gene            complement(5287..6534)
                     /locus_tag="VGD55_02055"
     CDS             complement(5287..6534)
                     /locus_tag="VGD55_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY0869155.1"
                     /translation="MELVLALLAPGQGAQVPGFLAPWLDLPGVADAVTWWSAVLDLDL
                     ARLGTSADADEIKDTKVAQPLLVGAGLAAASVLFPDGQPQFGIPGQPAGRLANTFDDV
                     ASNVAAVAGHSVGEITAAALAGVLSPESALVLVRERGRAMASAAAATPTGMTAILGGD
                     AAAVLAKLAELGLTAANVNAAGQVVAAGTLEQLAALAADPPEGARLRPLAVAGAFHTS
                     HMAPAVERLRQLATGLPTSEPGTRLLSNADGSVIQHGPEFVGHLVEQVAAPVRWDRCM
                     TTLGELGITAVIELPPAGTLVGLVRRALPDVEVLALKTPDDLDAARELIEKHRHDIEF
                     TATPAWRLLVAPLAGIFEAATAHLGDNLTPGAVIGRVVTRREAQEVVALQGGTLVEWL
                     VEDGDPVSPGQPLARLHPDVISE"
     gene            complement(6679..7767)
                     /locus_tag="VGD55_02060"
     CDS             complement(6679..7767)
                     /locus_tag="VGD55_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0869156.1"
                     /translation="MDETLPWYRAMPAEQRSWVGLVAQAGIAAFVEWFRHPAQRRAIT
                     GEIFGTAPRELARTVSLQQTVEMVRITIGVVEAAASTIAGPENAAELREAVLIYAREV
                     AFAAAQVYAQAAEARGAWDARLEAMVVDALMRGEVDEALRSRAAALGWGSPTSVTVVI
                     GTPPDDEPEAVVDGVRRAARHAGLDVLVAVQGDRLVAVLGGAPDPLRASRALLVQFGP
                     GPIVIGPPVPDLMSAARSAQAAVAGIVASKAWPTAPRPALASDLLPERAIDGDPAARR
                     QLIDEVYRPLLAAGGGLAETVAAYLAHAGSLEATARALYVHPNTVRYRLRRAADVTGF
                     AATDPREAFALHIAIVLGRLATAARPTD"
     gene            complement(7967..8419)
                     /locus_tag="VGD55_02065"
     CDS             complement(7967..8419)
                     /locus_tag="VGD55_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869157.1"
                     /translation="MPWQFGHRRRRPFVSSLDLHPNLAEILGILNQIPRLTGKDVARL
                     AHGWRDNAYLSIARADALGPDTPLVLEALAAFERVEAVFQEELFGWTLTESFSEEGSA
                     TTVRMPAVVNNALRAVRDALAAAYARPVLARSQYIALINPWHQAFSAD"
     gene            8535..9314
                     /locus_tag="VGD55_02070"
     CDS             8535..9314
                     /locus_tag="VGD55_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0869158.1"
                     /translation="MTIDPSGAIDVRPAADRPGSRADGVVSRHSFSFGPHYDPINTRF
                     GPLVTHNDDVLGVGSGFPEHAHRDLEIVTWVISGALSHADSLGGSAILRPGMVGRLST
                     GGGVRHSERNASAGDTRYIQMWVLPDAEGQPSYETADLSGALESGDFVTAASGTGGKV
                     GGTASGGAASGGRRAAVALRCNAAFLVARVPPGASAAIPAAPLLHVYVARGGVLLTVD
                     AEQVQLLSEDAARLTGAGQVWVEATTDAEVLVWAMGRAPDR"
     gene            complement(9408..>9632)
                     /locus_tag="VGD55_02075"
     CDS             complement(9408..>9632)
                     /locus_tag="VGD55_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869159.1"
                     /translation="YMRAVPDQIARWVPGDYSSLGTDGFGFSDTRAATRRFFHVDPAS
                     IVLQTLSELGRRGKIASDVAGKAAAQHGLI"
BASE COUNT         1498 a         3239 c         3340 g         1555 t
ORIGIN      
        1 cggcgcgatc ttcgtggtgg gcggcttctt cagctttacc gggtctaccg gcaagtcagc
       61 tgtcgaccac atcgcgaccg tgctggggac gctcggcctt gctgcgctgc cggtcttcgt
      121 cgggattgcg atcctgaagt atcggttgtt cgagatcgac cggttgatca gtcgcaccct
      181 ttcgtacgcg atcatcaccg gcctgctcgt cggcgtgtac gtcggcctga ttgcgttgac
      241 gacgcgtgcg ctgccgttgt cgtcgcccat cggcgtcgct gcctcgacgt tggcggctgc
      301 ggcattgttc acgccgctgc ggcggtgggt gcagcggatc gtcgaccgcc gtttcaaccg
      361 cgcgcggtac gacgccgagg cgaccgtcgc ggcgttcagc gcgggccttc gtgacgcggt
      421 cgatctcgac cgggtgcagc gcgacctgat tggcgtggtg aacctgtcga tgcaaccgag
      481 ccacgtgtcg gtctggctgc gcgagcaaag cccggtctga cgcggcgcgg gccggcggtg
      541 gatcgtggag tctcagcgct gctagagcaa ccggaatgct ccacgatgat ccgaacagcg
      601 ggggcgctgg cgggcgccgg gcgcgtcgat ggtggcgccg ggcggcgggc tggggcagaa
      661 tgtcaccact gggacacctg agcagatgcc gaggtcgctg gggaaggcgc atctcgtcca
      721 tctgacggag gttctggtga gcactcacgg tgtcttgtac gtgcactcgg cgacgccggc
      781 cgtcacgccc cacgtcgagt gggcagtggc cggcgttctt tcgacgcccg tgcgattcga
      841 ctggacatcc caacccgtgt cgccgggcag cctgcgcgct gaggtgacct ggatgggcga
      901 gaccgggtgc ggtgggcggc tcgcctcggc gctgctgggc tggccggtgc gtttcgaagt
      961 caccgaagac ccgagccccg gttgcgacgg cgagcgctac gccttcaccc cgggcctcgg
     1021 cctgttccac gcgatgacgt ccgcgaacgg cgacctggtc gtgccagaaa acaggctgcg
     1081 acagttgctc accgcgaacc tggtacaagt cgccgacgtc gcgtcagatc tgcggacgct
     1141 gctcggcgag ccgtgggacg ccgaactcga accgttcagg catgcgggcg aggggacgcc
     1201 ggtccgctgg ctgcatcaag tcgtctaagc ccggagggcg ccaggtggtg taacccgacg
     1261 acccgaggac cagaagagct agagcgggat gttcccgtgc gaaccgcgcc gcggaggtgc
     1321 ggccgcaatg gcgtcggcga tcatgctgcg ggtcgacgcc ggcgacacga cactgtcgac
     1381 cacaccaatc gcgaccgcct tgtcgatgct gccggccagc ttctcgtgct cagcagccaa
     1441 ctcgagttca agtgatggtc gcagcgcctc gccgacctcg gccagcttgc gccggtgcaa
     1501 gatgcgcacc gcagcaacgg atcccatgac ggcgacttcg gcgcctggcc acgcaagcac
     1561 agcggtggcg ccgagcgagc gcgagttcat cgcgatatac gcgccgccgt aagccttccg
     1621 ggtgatcagc gtgacgcgcg gcactgtcgc ttccgcgaat gcgtggagca gcttcgcacc
     1681 gcggcgcacc acgccgtccc actcttgtcc gagcccgggc aggtagccgg gaacgtcgac
     1741 cacgaccacc aatggcacgc cgaacgcatc gcacgttcgc acgaaacgcg cagccttctc
     1801 ggcgctcgtc gcgtcgaggc aaccaccgag gcgcagcggg ttgttggcca cgacgccgac
     1861 ggtgcggccc gcgagccggc cgaacacagt cacgatgttc ggtgcccact tggcgtgcag
     1921 ctcgacgccc ggggagtcga gcagaccgtt gatcagcggg tgcacgtcgt acgcacgctt
     1981 cgcaccttcg ggcaacagcg attcgaggtc gatgtcgacg aggtcggcat cgccgatttt
     2041 cccttgatta ccaaggagtt ccgtcaacac ccgcgcgcgt tcgagcgcat cttcttcgct
     2101 ctcggtgacg atgtgcacga cgccacttcg acggccgtga acctcggcgc caccgagacg
     2161 ttccatgtcg acgtcctcgc cggtgacgct gcgcaccaca tcgggaccgg tgacgaagat
     2221 gcgcccggca ggacccatga tgacgatgtc ggtcagggca ggtccgtagg ccgcccctcc
     2281 ggcggccggg ccgagcacga ccgagatctg cggcaccttg cccgacgcgc gggtcatcgc
     2341 tgcgaacacg cgcccgaccc cgtcgaggct ttcgacacct tcgcgaagac gcgctccgcc
     2401 cgagtgccac aagccgacga tcgggacgcc gtcggcgagc gcccgttcgt acgcttcgac
     2461 gatcgcggtg cagcccgcac tgcccatcgc gccaccctgg atcgtcgggt cgcacgcgaa
     2521 cgcgatggca ggtgtgccga gcatccggcc aatccccgca aggaccccgc tgtcgtcgtc
     2581 cgcagtcatt ggttcgaagc tgtcgtcgtc gaacagcgcc gtcagtcggg cgcgcggcga
     2641 acgcgggtcg agggcggtat cagccgtagt cgtcgtcacg gggttcctat caccagggaa
     2701 gtcaggcggg tttgatgacg agcaccacgt tgtggccacc gaagccgaac gcgttcgaca
     2761 tcgcggggcc gggtcgcaac gggcgcggat cgaccgtgac aacgtcgagt tcgacgtcgt
     2821 cgtcgcggtc gtcgaggttc aaggtcggcg gcacgatgtg ctcgttgatc gatagcaacg
     2881 tggcgatggc ttcgactgcg ccagcggcac cgagcaggtg tccgaacgtc gacttcggtg
     2941 cggtcacgac gacgctgtcg gcggctgagc cgaacacctg ccggatcgcc tttgcttcgg
     3001 cgacgtcgcc ggccggagtc gaggtcgcgt gcgcgttgat gtgcgtgatg tcggacggcg
     3061 cgagcccggc gttttcgagt gccgcgcgca acgccctgac aacaccgcga ccctcagggt
     3121 cgggcgccgc gatgtggtac ccgtcggacg tgatgccagc acccgcgatc tcaccgagga
     3181 tcgtcgcgcc gcgggcgcga gcgtgttcgg cggattcgag caccaccgca cccgcaccct
     3241 cgcccatgat gaatccgtcg cgacccttgt cgtaagggcg cgaggcacgc agcggttcgt
     3301 cgtttcgcgt cgacattgcc cgcatagcgg caaaagcagc cagcggcagc ggatgaatcg
     3361 ctgcctcggt accgccgcac acgacgacat cggcgcggcc ggtgcggatc atctcgagtc
     3421 cgtacgcgat cgcttcggcg cccgacgcgc aggcgctgac cggcgtgtgc acgccggcgc
     3481 gggcctgcag atcgagaccg accgctgcgg ccgggccgtt cggcatcagc atcggcacgg
     3541 tgtgcggcga cacgcgccgc ggacccttct cgcgcagtac gtcgtactgg ttgagcaagg
     3601 tcgtgacacc accgatgccc gatgcgaaga cgacgccgag gcgttctttc tccacgtcgg
     3661 gcttgcctgc ctgcgcccag gcctcgcgcg cagcgatgat cgcgaactgc tgcgagcggt
     3721 cgagcgtgcg ggcctcgacg cggtcgagca cgtcgagggg ttcgacctcg acgcgcgcag
     3781 cgatgcgcac ggggaggtcg gctgcccact cctcggtcag cgcgcccacg cccgacttcc
     3841 cggccagcag cgccgcccac gtgctggcga catcgccgcc gatgggcgtg gttgcgccga
     3901 ggccggtgat gaccacctgc cgtgcgttca cagctttctc ctctgtcacg ccactcaagc
     3961 ggtgacgcca gccttctcga tgtaggtgac ggcgtcgccg acggtcttca ggttcttgac
     4021 gtcgtcgtct gggatgcgta cgccgaactt ctcctcggct gcaacgacta cttcgaccat
     4081 cgacagtgag tcaacgtcga ggtcgtcggt gaagctcttg tccatctgga cgtcggaagc
     4141 cgggatgcca gcaacctcgt tcacgatctc cgcgagaccg gcgaggatct cctgctgggt
     4201 cagggccatg gggtgctcct tcattctgtg ggttgtgcgg ttgggtttga tccttgcagt
     4261 taagggattt cgacaacctg cgcagcgtag gtgagaccgg ctccgaagcc gatcagcagg
     4321 gcgggctggc cgctttcgac ctcgccgcgc tcgagcagtg cagatagggc caacggaatc
     4381 gaggcggacg acgtgttgcc ctgcgtctcc atgtcgcgcg cgatcgcgac agtgtcggga
     4441 agcttcaacg acttcaccag cgattcgacg atacgcaggt tcgcctggtg cggcacgaac
     4501 gcaccgagtt cttcgggagc cacgcccgcg gcctcgagtg cgcgccgcgc gacgggggcc
     4561 atctgcgtga ctgcccaccg aaacacggtc tggccggcca tcttcaatgc gactggtggc
     4621 agtgactcac cggtggcaag tgcgtgctgc acctgcgtgt agttgggctc ctgcgtgatg
     4681 gtcagcgcgt gcgaaccgtc gctgccccac accacagggc cgatgccggt ggtctggctc
     4741 gcgctcacaa ccgcagcgcc ggcgccgtca ccgaacaaga aggccatgcc acggtcgtgc
     4801 gggtcgatga actccgacat cttttccgaa ccgatcacga ccacgttgcg ggcactgccg
     4861 gcacggatcg cgtcgttggc aaccgagagc gcgtaagcga aaccggcgca tgccgcgttg
     4921 atgtccatcg cgcccgcgtt cacggctccg agccggtctt gcacctcact gctcccaccc
     4981 ggtgtttggt aggggtgcgt gcaggtggcg agcaacacga gctcgacgtc ggtagcggca
     5041 atgcctgacg acgcgagcgc ttttgatgcg gcgctggtcg ccatgtcgac gaccgtctcg
     5101 tcgggggccg cgaatcggcg ggtgatgatg ccactgcgct cgcgaatcca cgcatcggtc
     5161 gaatcgatgt cgcggcagat ttcttcgttg gtgacgatcc gggtcggtcg gtgctcaccg
     5221 atcgcgacga tgcgcgctcc gggcgaaccc gcctgcctgg tcagcgtttt catgcgcacg
     5281 cttccttcac tctgaaatca cgtccgggtg caagcgcgcg agcggttggc cgggagacac
     5341 cgggtcgccg tcctcgacga gccactcgac cagggttccc ccttgaagtg caaccacttc
     5401 ttgcgcctcg cgcctggtca cgacgcggcc gatgaccgcg cccggcgtga ggttgtcgcc
     5461 gaggtgggcg gtggccgcct cgaagatgcc ggccagtggg gcgaccagca agcgccaggc
     5521 cggggtcgcg gtgaactcga tgtcgtggcg atgcttctcg atcagttcgc gcgccgcatc
     5581 gaggtcatcg ggagtcttca acgccagcac ctcgacgtcc ggcaatgcac gtcgtacgag
     5641 gccgacaaga gtgcccgccg gcggaagctc gatgaccgcg gtgatgccaa gttcgccgag
     5701 cgtcgtcatg cagcggtccc agcgcaccgg cgcggcgacc tgctcgacga ggtgaccgac
     5761 gaactcggga ccgtgctgga tcacgctgcc gtcggcgttc gagagcaacc tcgtgccggg
     5821 ttccgacgtc ggcagacccg tcgcgagttg acgcaaccgc tcgacggcag gggccatgtg
     5881 cgaggtgtgg aacgcgccgg cgacggcgag tggacgcaat cgcgcgccct caggcggatc
     5941 ggctgccaac gccgcgagct gctcgagcgt gccggcggcg acgacctgcc cggcggcgtt
     6001 cacgttggcg gctgtgagac caagttcggc cagcttcgca agcaccgcag ctgcgtcgcc
     6061 accgaggatc gcggtcatgc cggtgggcgt cgcagccgca gcactggcca tcgcacgacc
     6121 gcgttcacgc acaagaacaa gagcagattc aggcgagagg acgccggcca gtgccgccgc
     6181 agttatctcc ccgacactgt ggccagcgac tgcagccacg ttgctggcca cgtcgtcgaa
     6241 ggtgttcgcg agtcgaccgg ccggctgacc agggatgccg aactgaggct ggccgtcggg
     6301 gaacagcact gaagctgcgg caagacccgc gcccacgagc aacggctgcg caaccttggt
     6361 gtctttgatc tcgtcggcgt cggcgctggt gcccaggcgg gccagatcga gatcgaggac
     6421 ggccgaccac caggtcaccg cgtcggcgac accgggaagg tcgagccacg gcgcgagaaa
     6481 gccgggaact tgggcgcctt gacctggcgc gagcaacgcg agcacgagtt ccaccttgcc
     6541 gcttatggcc gggctggtcg ctgcccggcg ggacgaagtc gctccggcga ctttgtagag
     6601 aacctacaac acgcccgccc ctctttcgct tccctcgact gtcagacagt tacgggtctc
     6661 gagaccctta agcgtccgtc agtcggttgg tcgcgcagcg gtggctaagc gtccgaggac
     6721 gatcgcgatg tgcagtgcga acgcctcgcg cgggtcggtc gcggcgaacc cggtgacgtc
     6781 ggcagcacgc cgcagccggt aacgcacggt gttggggtgc acgtagagcg cccgcgcggt
     6841 cgcttcgagc gagcccgcgt gggcgaggta ggccgccacc gtctcggcca ggccgccgcc
     6901 cgccgccaag agcgggcggt agacctcgtc gatcaattgg cgccgagcgg ccgggtcgcc
     6961 gtcgatcgcc cgctcgggaa gcaggtcgct cgccagcgcc ggccggggtg ccgtcggcca
     7021 ggccttcgac gcgacgatgc cggcaaccgc tgcctgcgca gaccgcgccg ccgacatgag
     7081 atcgggcacc ggtgggccga tgacgatggg tcctgggccg aactggacaa gcagcgcacg
     7141 cgacgcccgc agcggatcag gggcaccgcc gagcacggcg acgaggcggt caccctgcac
     7201 ggcaaccagc acgtcaagac cggcgtgtcg ggcggcgcga cgcactccgt cgacgacggc
     7261 ctcgggttcg tcgtcgggcg gagttccgat caccaccgtg accgatgtcg gggagcccca
     7321 gcccagcgcc gcggctcgtg agcgcagggc ttcgtcgacc tcgcccctca tgagggcgtc
     7381 gacgaccatc gcctcgaggc gcgcgtccca agctccccgc gcctcggcag cctgcgcgta
     7441 cacctgggca gcagcaaacg cgacttcgcg cgcgtaaatc agcacggcct cgcgcagttc
     7501 ggcggcgttc tcgggcccgg caatcgtcga cgccgcggct tcgaccacgc cgatcgtgat
     7561 gcgcaccatc tcgacggtct gttgcaggct cacggttctg gccagttcgc gcggcgcggt
     7621 accgaagatc tcacccgtga tcgcgcgccg ctgtgccgga tgcctgaacc actcgacgaa
     7681 cgcggcgata cctgcctggg ctacaaggcc gacccacgat cgctgctcgg ccggcatcgc
     7741 gcggtaccac gggagcgtct cgtccatctg agcgaccgca gctctcgcca ggtcgccggt
     7801 cgcgcggtcg agttcgcgtg ccgtcgccgt ccgggcatgt gccgcggaag atgccagcgc
     7861 ggcagctgcg gcactggggg atcgaggggc cacggcgcac cagtgtgccg cacggaacgg
     7921 gccgtctgag acctcgcggc cgtcgtcccg cccgaaatcg ttagagctag tcggccgaga
     7981 acgcctggtg ccatgggttg atgagcgcga tgtactgcga gcgggccagc accggcctgg
     8041 cgtacgcggc cgccagcgcg tcgcggactg cgcgcaaagc gttgttgacg acggccggca
     8101 tgcggaccgt ggttgccgag ccctcttcgc tgaaggactc cgtaagggtc cagccgaaca
     8161 actcttcctg gaagacggcc tcgacccgct cgaaggcagc gagcgcctca agcacgaggg
     8221 gcgtgtcagg tccgagcgcg tcggcgcggg cgatcgagag gtaggcgttg tcgcgccatc
     8281 cgtgagcgag ccgggcgacg tccttgccgg tcagccgcgg tatctggttc aagatgccga
     8341 ggatctcggc caggttcggg tgcaggtcga gcgagctcac aaagggcctg cgtcggcggt
     8401 gcccaaactg ccacggcatc gtcggcctcc tccaagtgcg tggttctgta cagaaattcc
     8461 tcggcatgat cgcgggtgca gtggagcgtt cggcaccggc aaaatgtccg tatgcgcagt
     8521 ttcgggaccg accggtgacc atcgacccca gcggcgccat tgacgtccgg ccggctgccg
     8581 atcgccccgg gagtcgtgcc gacggcgtgg tctcgcggca ctccttttcg ttcgggccgc
     8641 actatgaccc gatcaatacc cgattcggtc ccctggtaac gcacaacgac gacgtgctgg
     8701 gggttggttc aggcttcccc gaacacgctc atcgcgacct cgagatcgtg acctgggtga
     8761 tttccggcgc cctttcccat gcggacagcc tcggcgggtc ggcgatcctg cgccccggca
     8821 tggtcggccg cctctcgacc ggtggcggcg tgcggcatag cgagagaaac gcgtccgcag
     8881 gtgacacgcg ctacatccag atgtgggttt tgccggacgc cgaggggcag ccgtcctacg
     8941 aaaccgcaga cctgtcggga gctctcgaga gcggcgactt cgtgaccgcg gcgtcaggca
     9001 ccggcgggaa ggtaggcggg acggcttcgg gcggcgcggc gtctggcggc cggcgggcag
     9061 ccgtggccct tcgctgcaac gcggccttcc ttgtggcgcg ggtgcccccc ggcgcctcgg
     9121 ccgcgatccc agccgcgccc ctccttcacg tgtacgtcgc gcgcggcggc gtgctgctga
     9181 ccgtcgatgc agaacaagta caactgctca gcgaggacgc ggcgcggctg accggggcgg
     9241 gccaggtgtg ggtcgaggcg acgacggacg ccgaggtact cgtctgggcg atgggccgcg
     9301 cacccgaccg atgaccgacc ctgaatgtcg aagttggaag ccctgggacg ctctagatgg
     9361 cgtcgtcggg cttccaactt cgccgtcggc cccttgatga gcgcgcccta gatgaggccg
     9421 tgttgcgccg cggccttgcc ggcgacgtcg gatgcgatct tgccgcggcg gccgagctcg
     9481 ctcaaggtct gcagcacgat cgacgccggg tcgacgtgga agaaccggcg cgttgctgct
     9541 cgggtgtcgg agaagccgaa cccgtcagtt ccgagcgatg agtaatcgcc aggcacccag
     9601 cgagcgatct ggtcgggtac cgctcgcatg ta
//
LOCUS       DASWVF010000119         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_487386, whole genome shotgun sequence.
ACCESSION   DASWVF010000119 DASWVF010000000
VERSION     DASWVF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487386"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..862
                     /locus_tag="VGD55_02080"
     CDS             179..862
                     /locus_tag="VGD55_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0869160.1"
                     /translation="MPTELRVLSYNVRSLRDDAQAVSTVIRACEPDVVCIQEAPRFLR
                     WRSKCAALARESGLVVVTGGRTAGAMLLLASLRVRVVRTQDILLSRKKGLHQRGIALA
                     TLELGGSQFSVGSIHLDLHAAERRRHVDEIVERASSCQPPVIIAGDINEEPDGPAWRA
                     LAQSYPDAFELAPLGGANTYSATEPVRRIDGIFVDRVVTVKSCGVPDVPGIERASDHR
                     PVLAVLQLP"
     gene            complement(866..1804)
                     /locus_tag="VGD55_02085"
     CDS             complement(866..1804)
                     /locus_tag="VGD55_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869161.1"
                     /translation="MQHPVAFSGKIDDMPGRDNGFVARHYVPLADLDPRLADAMLEAL
                     RAEGIAAYVVPSTGRLGGYLEVHLPERPHDRLWVDREHHDRAAALLNLHGDRPEIIAT
                     PTDDSTGAGDFPDALAGDATEPQPTDAADSADSADDVWARLVADFDLPSEQADGRWPL
                     AEDLPVDADGPAIREAGRGSGPGQIPHGLRPGDGAGTGGTPVPRRIVRRVEPGFGPTV
                     PESLPSEYDPLSILDEHFVPDPPPPLPRLRRGTRWALASILLGVVLIVGRAFWDTIPD
                     AVALGVLAVVGGFIGLVAMMREDRPSDSDPDDGAVV"
     gene            1897..>2264
                     /locus_tag="VGD55_02090"
     CDS             1897..>2264
                     /locus_tag="VGD55_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0869162.1"
                     /translation="MFYWFLKWTLGASLRLVFRPKVVGVENVPPKGGAIIASNHLSFS
                     DSFFAPLVIPGRKVTFMAKAEYFNRRGIKGTLSRWFFRSIGQLSVDRSGGKASASGLR
                     TGLEVLEAGELLGIYPEGTRS"
BASE COUNT          368 a          735 c          778 g          383 t
ORIGIN      
        1 ctgtccggcg gggccgccga cttgctggat tcatgacgag ggcgcaccct cagtactcgc
       61 aacgactgag acgggattct tcaaggagac tccattgcgg atcccgactg tcggtactga
      121 tcacccgatg gcggagccac ctccatcggc accgcacctc gtcaagtaag ggcctttgtt
      181 gccgactgaa cttcgggttc tcagctataa cgtgcggtcg ttgcgcgacg acgcgcaggc
      241 cgtttcgacg gtgatccgcg catgcgagcc cgacgtcgtc tgtattcaag aggcgccgcg
      301 ctttctgcga tggcgttcga aatgtgcggc cctagcgaga gagtcgggct tggtggtggt
      361 taccggcggc cggacggccg gcgcgatgtt gctgctcgcc tcgctgcgcg ttcgcgtagt
      421 gcgaactcag gacatcctgc tgtcgcgcaa aaaaggcttg catcaaaggg gtatcgccct
      481 cgcgacgctc gaactcggtg gctcgcagtt ctcggtcggc agcatccacc tcgacctcca
      541 cgcagccgag cggcgtcgcc acgtcgacga gatcgtcgag cgcgcgtcgt cgtgccaacc
      601 gcccgtcatc atcgccggcg acatcaacga agaacccgac ggacccgcgt ggcgagcgct
      661 cgcgcagagc tatcccgatg cgttcgagct cgccccgctc gggggtgcga acacgtatag
      721 cgcaaccgaa ccggtgcgca ggatcgacgg catcttcgtc gaccgcgttg tcacggtgaa
      781 gagctgcggt gtgcccgacg tcccgggtat cgagcgagcg agcgaccatc gcccagtgct
      841 cgcggtgctg cagctgccgt aacagctaga cgacggcgcc gtcgtcgggg tcggagtcgc
      901 ttggccggtc ttcgcgcatc atcgcgacca acccgatgaa acctccaaca acggcgagga
      961 cgccgagggc aaccgcgtcg ggaatcgtgt cccagaatgc ccgcccgaca atcagcacca
     1021 cgccgagcag gatcgatgcg agtgcccatc gcgtgccccg gcgcaatcgg ggcagcggcg
     1081 gtggcggatc gggcacgaag tgttcgtcga gaatcgacaa cgggtcgtac tcactgggca
     1141 gcgattcggg caccgtcgga ccgaaccccg gctcgacccg gcgaacgatg cgacgcggca
     1201 ccggagtacc gccggtaccg gcgccgtcgc caggtcgaag gccgtgcggg atttgccctg
     1261 ggccgctacc acggccggct tcgcgaattg ccggcccgtc ggcgtccacc ggaagatctt
     1321 ctgcaagcgg ccaccggcca tcggcctgct ccgacgggag gtcgaaatca gcgacgagcc
     1381 gtgcccagac gtcatcggct gagtccgccg aatcggctgc gtcggtcggc tggggttcgg
     1441 tggcgtcgcc ggccaacgca tcggggaagt cgccggcccc ggtggagtcg tcggtcggcg
     1501 tggcgatgat ctccgggcga tcgccgtgca ggttcaggag cgcggcggcg cggtcgtgat
     1561 gctcgcgatc gacccacaac cggtcatgcg ggcgctcggg caggtgcacc tcgaggtagc
     1621 cgccgaggcg gcccgtgctc ggcacgacat acgcagcaat gccctcggcg cgcaacgcct
     1681 cgagcatcgc gtccgcgagc cgcggatcga gatccgcgag cggcacgtag tggcgggcga
     1741 cgaacccgtt gtcgcgacct ggcatgtcgt caatcttgcc cgagaatgca accgggtgct
     1801 gcatgctgtc gcgggctatc tgcgacggag ggttcgcaga ggtatggtgc gcccagttgc
     1861 gtcaagggtg gccgtcaagg gatgaaggtg cgctaggtgt tctactggtt tctcaagtgg
     1921 accttgggag cgagccttcg cttggtgttc cgtccgaagg tcgtcggggt cgagaacgtg
     1981 ccgcccaagg gtggcgcgat catcgcgagc aaccatctgt cgttctccga ctcgttcttc
     2041 gcgccgttgg tgattcccgg gcgcaaggtc acgttcatgg ccaaggcgga gtacttcaac
     2101 cgccgcggca tcaagggcac gttgagccgc tggttcttcc gcagcatcgg gcagttgtcg
     2161 gtcgaccgct cgggcggcaa ggccagcgcc tcggggctgc gcaccggcct tgaggtgctt
     2221 gaagccggcg agctgctcgg catctatccc gagggcaccc gctc
//
LOCUS       DASWVF010000120         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_156230, whole genome shotgun sequence.
ACCESSION   DASWVF010000120 DASWVF010000000
VERSION     DASWVF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156230"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VGD55_02095"
     CDS             <1..701
                     /locus_tag="VGD55_02095"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869163.1"
                     /translation="KPLRRVASHVMHRASLVATVSEDLQRQILEFDPRLHTAVLRLPI
                     GEQPPPVPMPSDPPIRLLAAGRLSREKGFDVLIEAMRIAVDHGLDVRLDIVGSGPERR
                     QLALLAAPLGDRVQLIPALPHAELMRRLDDVHALVAPSRREGLGMVALEAFTRGRPVV
                     ASRVGGLAESVEDGVDGYLVPPEDPIALAAAIARLPFGPPKGTATLRHSPIEVGRSHL
                     EAYASLVSPMVKSR"
     gene            911..>2210
                     /locus_tag="VGD55_02100"
     CDS             911..>2210
                     /locus_tag="VGD55_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869164.1"
                     /translation="MTTATRRLRRASIAGLATFATAAAVVAGAGSAFGFAAGATSGGG
                     SLTPGASVVVFPGVASQAVNSLTLALKDGNTTAGNGWASGDSVTLQVNNQTAGTELCN
                     TAGNTNAKASLAVPSVAASDSIGPVAFTGFSVSQAPGANCNVNDTITIHLTAGAPADL
                     GTTTFTISGLSLTLGTNFPTTPTAVTVQATASNNAPFGTVGAGTQTASVSVATVGTNS
                     VSVSPVVGAANGTSGVAISPIVVTDVTGGTITVGLKFTAAAGDFFATSGTLAVPSGVT
                     VTGPSETLPAATQTYLIAAGSVPAGGKFTLTGATVNLANAASSHKVTVGTAAGEATLV
                     GAAAEYATTAAQVRTFAGVDRYGTARALYHAAFAARTVAVITSGANYPDALSADVIAK
                     KNTTGVLLTDPNALSQDTQQELQADPIATVYIIGGPAAVSQ"
BASE COUNT          370 a          743 c          701 g          396 t
ORIGIN      
        1 acaagccgct gcgccgggtt gcgtcgcacg tcatgcaccg cgcttcgctt gtagcgaccg
       61 tgtcggaaga tttgcagcgg cagatcctcg agttcgaccc gcgactgcac accgccgtgc
      121 tgcggttgcc gatcggcgaa cagccgcctc cagttcccat gccgtcagac ccgccgattc
      181 ggttgctcgc cgccggccgg ctctcacgcg aaaagggttt cgatgttctg atcgaggcca
      241 tgcgcatcgc ggtggaccac gggctcgacg tccggctcga catcgtgggg tcggggccag
      301 agcggcggca gcttgcgctg ctagcggctc ccctcggcga tcgggtgcag ttgatcccgg
      361 ccctgccgca cgccgagtta atgcgccggc tcgacgacgt gcatgcactg gtggctccgt
      421 cgcgtcgaga gggcctcggg atggtcgctc tcgaggcgtt cacgcgcggt cggccggtgg
      481 tcgcgagccg cgtcggcggg ctggccgaga gcgtcgagga cggcgtcgac ggctatctcg
      541 tgccgcctga agaccccata gctttggccg cggcgatcgc ccgcctgccg ttcggtcctc
      601 ctaagggcac cgcaacgtta agacactctc caattgaggt tgggagaagc cacctcgagg
      661 cttatgcatc ccttgtctcg cccatggtca agagtcggta ggttcagagc aaccagatcg
      721 gacgtatgtc cgttttgggc taaacaacca ccgacacgac gtaagtcgtg ggtggggcgt
      781 ctggcttccg caagcgcaga acctgtaggg tcccgaatcg agcaagtttg ctcgggtatc
      841 ggcggggctg tacagggctg cacctacggg ctgccacctc gccaccacca cttctctgat
      901 ggaggcaaga gtgacaacag caaccagacg gttgcgtcga gccagcattg ctggtctcgc
      961 aacgttcgcg acggcagcag ccgtcgtagc gggcgccgga tcggcgttcg gattcgcggc
     1021 cggcgcgacc agcggtggcg gctcgctcac ccccggtgcg tcagtcgtcg tgttcccagg
     1081 cgtcgcgtcg caggcggtca acagcctcac cctcgcgctg aaggacggca acaccacggc
     1141 tggtaacggc tgggccagcg gcgactcggt cacactgcag gtaaacaacc agactgcagg
     1201 caccgagctc tgcaacaccg cgggcaacac caacgccaag gcatcgctgg cggttccgtc
     1261 cgtcgccgcg tctgactcga tcggcccggt tgctttcact ggcttcagtg ttagtcaggc
     1321 ccccggcgcg aactgcaacg tcaacgacac gatcacgatt cacctgacgg cgggcgcgcc
     1381 tgctgacttg ggaaccacga ccttcacgat ctctggtctt tcgctcacgt tggggaccaa
     1441 cttcccgacg acgccgaccg cggtcactgt ccaggcaacg gcgagcaaca acgcgccgtt
     1501 cggcactgtc ggcgccggta cgcaaaccgc gtcggttagc gtcgccacgg ttggcaccaa
     1561 ctccgtctcc gtctcacccg tcgtgggtgc ggcgaacggt acgagtggtg tagcgatcag
     1621 tccgatcgtg gtcaccgacg tcacaggcgg caccatcacc gtgggtctca agttcacggc
     1681 cgccgctggt gacttcttcg ccacttcggg cactctcgcg gttccgtcag gcgtcaccgt
     1741 cacggggccg tctgagacac tccctgctgc aactcagacc tacctgattg ccgctggctc
     1801 cgtacctgcg ggtggcaagt tcaccctcac cggtgcaacg gtcaatctcg ccaacgcggc
     1861 gtcctcgcac aaggtcaccg ttggtactgc ggccggcgag gcaacactcg tcggtgcggc
     1921 agctgagtac gcgacgacgg ccgctcaggt ccgtacgttc gcgggcgtcg accgttacgg
     1981 cacggcgcgg gctctgtacc acgccgcgtt tgcagcacgc acagtcgctg ttatcacgag
     2041 tggcgcgaac taccctgacg cgttgtctgc tgacgtgatc gcgaagaaga acaccactgg
     2101 tgtcctcctg accgacccga atgcgttgtc ccaggacacg cagcaggagc ttcaggctga
     2161 cccgatcgca acggtgtaca tcatcggtgg acccgctgca gtcagtcaga
//
LOCUS       DASWVF010000121        10969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_487427, whole genome shotgun sequence.
ACCESSION   DASWVF010000121 DASWVF010000000
VERSION     DASWVF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487427"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /gene="modA"
                     /locus_tag="VGD55_02105"
     CDS             complement(<1..676)
                     /gene="modA"
                     /locus_tag="VGD55_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0869165.1"
                     /translation="MNRHQSAAVIATVTIALTLSACSSSKKAAAPSSSPAASATSAPA
                     ATSPAATSGVEQSPSDIATPVSGLSGNVVVFAASSLQVTFTTLITNFEAANPGLKVTP
                     SYGGSDTLAAQITQGAPVDVFAAASTTTMGTVTTAGDAVGTPQVFAKNELEIAVAPNN
                     PDHIATLADVTKSGVKLAVCAPTVPCGAAATKAFAAANLTPHPVTLEQNVTAVLTKLE
                     LGEVDAG"
     gene            complement(721..1122)
                     /locus_tag="VGD55_02110"
     CDS             complement(721..1122)
                     /locus_tag="VGD55_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0869166.1"
                     /translation="MSTYRIADAARLLGVSDDTARRWAESGKFATTNDEAGRLAIEGA
                     ELARFATTLNDPLEPGPAVQASARNRLVGIVTRVIRDTVMAQVELQCGGHRVVSLMSR
                     EAADELGLEPGVLAIASIKSTNVVVELPQHR"
     gene            complement(1195..1971)
                     /locus_tag="VGD55_02115"
     CDS             complement(1195..1971)
                     /locus_tag="VGD55_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0869167.1"
                     /translation="MTQELDQTAQNRLADAGKFFTSDLSVSEFTLLEHMGFDAIELVM
                     GVSVFHVGFQPGRGMQAGEYQVLSQAMYTARMNAINRLVAETVKVGGDGVIGVKLGYR
                     AHSAEALEFTAIGTAVRHRDGAAAHRAPSGAPFTSHLDAKELFQLLNVGWSPVSFVLG
                     TCVYHIAVQGVMQTMRQVGQNIELPQWTQGFYDARELAMTRLQAEGERDGAQGVVGVT
                     VVTSEWVWGGHTLEFYVSGTSVRRISNERGALPPLVVPVG"
     gene            complement(2008..2175)
                     /locus_tag="VGD55_02120"
     CDS             complement(2008..2175)
                     /locus_tag="VGD55_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869168.1"
                     /translation="MADHDKTDPKDLEAEAKAHIAEMPAETALGNADPEGGGEGTPAE
                     FVKKHEGHGGR"
     gene            complement(2217..2852)
                     /locus_tag="VGD55_02125"
     CDS             complement(2217..2852)
                     /locus_tag="VGD55_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869169.1"
                     /translation="MPNLAHRHHLAGQADRARHADLPCYGLPLEHPAARSLGGMSGVV
                     GSDHLMHASTMSVQHAGTAGASLQVVVQRHGGYHEVPPMARLALPLVRDRDGFADPRW
                     AARTVEEFHQGQERALAELAPQPLDVRVDGVTRTGDVVSIGNDWAAWIPMPDQEFDLE
                     LRGRGWPRDDLALVVIEDLEPYIAGSRAGVGPLLLPRSPRLPQLMRNRRRP"
     gene            complement(2809..4269)
                     /locus_tag="VGD55_02130"
     CDS             complement(2809..4269)
                     /locus_tag="VGD55_02130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0869170.1"
                     /translation="MGLEAATDEQRNPYLDLIRVLAIAAVVVGHWIASGVTYRHGEFD
                     GVDVLGVVSWTSWLTLIFQVVPLFFLVGGYSNALSWSRHQAGGSAFSWIRRRVLRLIM
                     PTAAYLAVVSLAVVLCNVAGADHADLQQAAWALALHLWFLAVYLVLLLLTPALLAAHH
                     RYGITGLAVTTALAVAIDVGVIEAHWHVVGWVNYVLVWGTFHQLGFAWYEGFFQGLLA
                     GRRGRPFVLTAGAISALVVLIWWGPYPVSVVGVPGARIQNASPPSAALLAFGFAQVGL
                     VLIAAPWATRWLTRPDRIRIRTLIGRANAMTMPVYLWHMVPVVLVSVVAYPNHLLAQP
                     SIGSGAWWAQRIEWIAMLSVVLVGVLALVALIANAWPRPSVPSGPRATLPRNIAMALF
                     GIGVGVSVAALGRLAVQGFAPNGSFDIVAVAGFAIGTVLIALPRPPKLVGQSPREQPT
                     ATGQSPPSAASTPPVVVAGRLGVAEPRTQASPRGAG"
     gene            complement(4393..5430)
                     /locus_tag="VGD55_02135"
     CDS             complement(4393..5430)
                     /locus_tag="VGD55_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869171.1"
                     /translation="MFTPIVRRVAVAAIAGVTAIGIAAVPAFATTTKPVTRLAGTDRI
                     GTSIAISTQEFPAAHSAKNVVLTAAYTFADAMSAGPLGKLFTAPLLLTSPGQLDSATL
                     TEIERVMPVPATTPGTTTTGCTTPAPANAVYVIGGYAAIAQEIDTQLNTAGFNVVRVA
                     GADRFATSVDVAQCEGSPSTVFLATGDIFADALSAGPAAASKGGSVLLTAATVMPASV
                     DTYLAGLTSPTVYAVGQAAHVADPSATPIVGGDRYATAALVASEFFTSPTLVGIATGA
                     NFPDALAGGAFMGMVGGPVLLTGPLTLQSADGLYIGQNHANLTQAFLFGGTLTLSALI
                     ATQASAALNQS"
     gene            complement(5545..6621)
                     /locus_tag="VGD55_02140"
     CDS             complement(5545..6621)
                     /locus_tag="VGD55_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0869172.1"
                     /translation="MTSAASIKDVAAHAGVSVGTVSNVLNRPERVAEDTRNRVQTAIK
                     ALGFVRNEPARQLRAGRSRTIGLLVLDAANPFFADVSRGAEEAAAKAGLAVILCNSGQ
                     DAAKETSYLNLMAEQRVQGLLLTPVDADPVRLARLVERGMPVVLLDRRAEGDGMCSVS
                     VDDVSGGELALTHLLEIGHERVAFVGGPTTIAQVKDRRAGARNALSRAGRDPDSLLCI
                     DTASMTVGAGREAGERLGGLPANRRPTAVFCANDLLALGLLQELIRQGLNVPNDVAIV
                     GYDDIEFAGAAAVPLTSVRQPRHLLGKAAAELLLEETQNPAGHVHRQIEFEPDLVVRA
                     STRTRTAPMAVTQVKKARSARPTT"
     gene            6864..8405
                     /locus_tag="VGD55_02145"
     CDS             6864..8405
                     /locus_tag="VGD55_02145"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0869173.1"
                     /translation="MTATDTTGVPSSRETPLLELAHATKSFGAVKALEDGSVRLYAGE
                     AHALVGENGAGKSTLVKILAGVHHPDSGELLIGGEPIVLNGPAASRAAGVSIIYQEPT
                     LFPDLTVAENIFMGRQPIGRGRRIDKAAMSRAAREVFARLGVELNPGRVARGLSVADQ
                     QIVEIAKAISFNARIIVMDEPTAALTSVEVARLFDVVRTLRAQGSAVLFITHRLEEAF
                     AICQRVTIMRDGRFVRTAPIEDVTIDDIIRSMVGRDLDQLFPKSETAPGEVVLAVERL
                     TREGVFTDVSFSVRRGEIVALAGLVGAGRSEVARAIFGIDRRDAGRVLVRGQELPNGS
                     PRAAMAAGIALVPEDRRQQGLVMDLGIDQNVSLASLSSLERFGLIRRSSERALAAEWA
                     LKLQLKYSRLRNPASTLSGGNQQKTVLGKWLARHPTILIIDEPTRGIDVGTKAEVHRL
                     LDQLVADGVAVLMISSELPEVLGMADRVLVLREGRLVAELSRADADEASIMRAATGQS
                     VGSAA"
     gene            8411..9436
                     /locus_tag="VGD55_02150"
     CDS             8411..9436
                     /locus_tag="VGD55_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869174.1"
                     /translation="MATAPPSVTKVGRAESLLRARELGIGIAIVIVFGLTTLKNPAFA
                     HALSVQQLLTGASLIALLAVGETLVIITRNVDLSVGSVLGLSAYIVGNTFAHHPHTAP
                     IVGFAIGIVVGAVFGAINGIIVTVLRVPSLVVTLATLYIIRGLDAMIVNGKQIDPSSV
                     PKAFQAVGNRNIFGVPWLAIIVAIVIAIAGYAMRSFRASRDIYAIGSNPEAAALAGVP
                     IGRRVFTAFLISGALAGLAGALFLSEVATVDATGGTGYELLVVSAVVVGGVAIFGGSG
                     TVLGAALGALLLNTINQALGALKLSSFWDQAVAGGLLLAAIAFDRWLGLRIAKRMKSA
                     RGEHRVV"
     gene            9426..10454
                     /locus_tag="VGD55_02155"
     CDS             9426..10454
                     /locus_tag="VGD55_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869175.1"
                     /translation="MSSDLATAPPRAQHVDTGLPSWRWLLRWETALVFIFVGTLFFGA
                     SESPHFMTHTNVFFIGLNMGEIAIMALPLTLIVITGEIDLSVAAMLGLSSTMLGYLYE
                     HHWPIGLAMVAVLVIGAVGGAFNGFLVTKLGLPSIAVTIGTLALFRGIAEIVLGANSV
                     VKFPTGLTNIGVKPVPHTELSYSAVIFIVLAIVYGVILHATPAGRSMIAIGLQSEAAF
                     FAGIRVKRIKFILYTMSGVVCAFAGILWTYRLSSSRYDNGLGLELDVVAIVLFGGVSI
                     FGGRGSIIGVVLAVIVFGSIKEALTLMNVSAQAQNIVTGGLLLISVILPNVASSVRRV
                     RARAGLRT"
     gene            10549..>10969
                     /locus_tag="VGD55_02160"
     CDS             10549..>10969
                     /locus_tag="VGD55_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869176.1"
                     /translation="MIHGSRARRLSAVTFAVALVTLAAACSSSKKAAPAASSAAPTTS
                     AAAPATSAAPATTSAAAPATSAAAAPTSAAAGSGAPANIKKGLVVYFIPKDTQNPYEV
                     IADKGGAAALAELGGKVVVSSGTADTAAAQIPSIQAAI"
BASE COUNT         1806 a         3515 c         3716 g         1932 t
ORIGIN      
        1 tgccggcgtc gacttcaccg agctcgagct tggtcagcac tgccgtcacg ttctgctcga
       61 gcgtgactgg gtggggggtg aggttcgcgg ctgcgaaagc cttcgtggcg gccgcgccgc
      121 acggcacggt tggcgcacac actgccaact tgaccccgga cttcgtcacg tcagcgaggg
      181 tcgcgatgtg gtcggggttg ttgggcgcca cggcgatctc gagctcattc ttggcgaaca
      241 cctgcggcgt cccgactgca tctcctgctg tggtaaccgt gcccatcgtt gtcgtgctcg
      301 ccgcggcgaa gacgtcgaca ggtgcgcctt gggtgatctg ggcggcaagc gtgtcggagc
      361 cgccgtagga cggcgtgacc ttcaggcccg ggttcgccgc ttcgaaattc gtgatcaacg
      421 tggtgaaggt cacctgcaac gacgacgcgg cgaacacgac cacgttgccc gaaagcccgg
      481 agacgggagt tgcaatgtcg gacggcgact gttcgacgcc acttgtggcc gccgggcttg
      541 tcgcggccgg agcggaggtg gccgacgctg ctggcgacga tgacggggcc gccgccttct
      601 tcgacgaact gcacgcgctg agggtcaaag cgatcgtgac agtcgcgatc acggcggcgg
      661 actggtggcg gttcatcgat tctcctgtag cgattcgggg acgccgggtg gcgcccgttg
      721 ctagcggtgc tggggaagtt ccaccaccac gttggtcgac ttgatggaag cgatcgccag
      781 cacgccgggt tcgaggccca actcgtcggc tgcttcgcgg ctcatcaggg agaccactcg
      841 atgcccgcca cattgcagtt cgacttgcgc catgacggtg tcgcggatca cgcgggtgac
      901 gatgccgacc aaccgattgc gcgcagacgc ctggacagcc ggaccaggtt cgagtggatc
      961 gttcaaggtg gtcgcgaagc gagcgagctc ggcgccctct atcgcaaggc ggcccgcctc
     1021 gtcgttggtc gtcgcgaact tgcctgattc ggcccagcgg cgagcagtgt cgtcgctgac
     1081 ccccaacagc cgcgcggcgt cagcgatgcg gtacgtcgac atggggtcac cctagccgca
     1141 caagcggcga gaaagcgatc tcgtttgccg caggtgcggg agggcgctgg attgttagcc
     1201 gaccgggacg acgaggggtg gcagcgcgcc ccgctcgttg ctgatgcgcc ggaccgaggt
     1261 gcctgacacg tagaactcca gcgtgtggcc gccccagacc cactcgctgg tcaccacggt
     1321 tacgccgaca actccttgcg cgccgtcgcg ttcgccctcg gcctggaggc gggtcatcgc
     1381 aagttcgcgg gcgtcgtaga aaccttgggt ccactgggga agttcgatgt tctgcccgac
     1441 ctgacgcatc gtctgcatga cgccttgaac cgcgatgtgg tagacgcagg tgcccagtac
     1501 gaacgacaca ggggaccagc cgacgttgag gagttggaac agttccttcg cgtcgaggtg
     1561 actcgtgaac ggcgcacccg acggtgcgcg gtgagccgcg gcaccgtcgc ggtgacgcac
     1621 cgccgtgccg atggcggtga actcgagtgc ctcggcgctg tgggcgcgat acccgagctt
     1681 gacgccgatg acgccgtcgc ccccgacctt cacggtctcg gcgaccaggc ggttgatcgc
     1741 gttcatccgc gccgtgtaca tcgcctgcga caagacctgg tactcgccag cctgcatgcc
     1801 gcgcccaggc tggaagccga cgtgaaagac ggagacgccc atcacaagtt cgatcgcgtc
     1861 gaaccccatg tgttcgagca gggtgaactc ggaaaccgag aggtcggagg tgaagaactt
     1921 cccggcgtca gcgagccggt tctgtgcggt ctggtcgagt tcttgtgtca tggggccacg
     1981 ctagcaatgc agcgactact tgccgctcta cctgcccccg tgaccttcat gcttcttgac
     2041 gaactctgcg ggcgtgccct cgccgccacc ctcgggatcg gcgttgccaa gcgcggtctc
     2101 ggcaggcatc tcggcgatgt gcgccttcgc ctcggcttcg aggtccttgg ggtcggtctt
     2161 gtcgtggtcg gccatggcgg gctccctagt gggttgtctt gtgctgaaag gctacgtcag
     2221 ggacggcggc gattgcgcat gagctgcgga aggcgggggc ttcggggcag cagcaacgga
     2281 ccgaccccgg cgcgcgaacc ggcgatgtaa ggctcgagat cctcgatgac cacgagcgcg
     2341 aggtcgtcgc ggggccaccc gcggcctcgc agctccagat cgaactcctg gtcgggcatc
     2401 gggatccagg ccgcccagtc attgccgatg ctcacgacgt cacccgttcg tgtcacgcca
     2461 tcgacgcgga cgtcgagggg ctggggcgcc agctcggcca gcgcccgctc ttgtccttga
     2521 tggaattcct cgacggtgcg tgccgcccag cgcgggtcgg cgaacccgtc gcgatcacgc
     2581 acgagcggca gcgcgagccg ggccatcggt ggcacctcgt gatacccccc gtggcgctgc
     2641 acgacgacct gcaacgaggc gccagctgta cccgcatgct gcaccgacat cgtcgacgcg
     2701 tgcatgaggt ggtcgctgcc cacgacccct gacatcccac cgaggctgcg cgctgccgga
     2761 tgttccagtg gcaagccgta gcacggcagg tcggcgtggc gagcccgatc agcctgcccc
     2821 gcgaggtgat gcctgtgtgc gaggttcggc aacgcccagc cgaccagcca ccaccaccgg
     2881 tggtgtcgat gcagcactcg gcgggctctg gcctgttgcc gtcggctgct cacgaggcga
     2941 ctgtcccacg agtttgggtg ggcgtgggag agcaatgagg acggtgccga ttgcgaaccc
     3001 cgcgacggcg acgatgtcga acgaaccgtt cggtgcgaag ccctggacgg cgaggcgtcc
     3061 caacgccgca acgctgacgc ccaccccgat cccgaacaac gccatcgcaa tgttgcgggg
     3121 gagtgttgct cgaggccccg acggcaccga cggcctgggc cacgcatttg cgatgagtgc
     3181 gacgagagcc agcaccccga cgagcacgac gctcagcatc gcgatccatt cgatgcgctg
     3241 ggcccaccac gcgcccgagc cgatcgaggg ctgggccagc agatgattcg ggtaggcgac
     3301 gactgacacg agcacgaccg gaaccatgtg ccagaggtag acgggcatcg tcatcgcgtt
     3361 ggcgcgtccg atgagcgtgc gtatgcggat gcggtctggt cgggtgagcc acctcgtggc
     3421 ccagggcgcg gcgatgagta cgaggccgac ctgtgcgaac ccgaacgcaa gcaaggctgc
     3481 tgagggcggc gacgcgtttt ggatccgcgc gccaggtact ccgaccacgc tgacgggata
     3541 cggaccccac cagatcagta cgaccagggc cgagatcgcg ccggcggtca gcacgaacgg
     3601 tcgccctcgt cgtccggcaa gaaggccctg gaaaaaccct tcgtaccagg cgaatccgag
     3661 ctggtggaag gtgccccaca ccaacacgta gttcacccaa ccgacgacgt gccagtgtgc
     3721 ttcgatcacg ccgacgtcga tagccaccgc gagtgccgtg gtaacagcga gaccagtgat
     3781 gccgtaccgg tgatgcgccg cgagcagggc tggggtcagg agcaagagaa cgaggtagac
     3841 cgcgaggaac cacaggtgaa gggcgagcgc ccacgcggcc tgctgaaggt ctgcgtggtc
     3901 ggccccggcg acgttacaca ggaccaccgc gagcgacacc accgcgaggt acgcggccgt
     3961 cggcatgatc aggcgcaaca cccgacgacg gatccatgag aacgccgagc cgccggcttg
     4021 gtgcctgctc cacgacaggg cgttggaata gccacccaca aggaaaaaca gcggcacgac
     4081 ctggaagatg agggtcagcc acgaggtcca tgacacgacg ccgagcacgt cgacgccgtc
     4141 gaactcaccg tgcctgtagg tgacaccgga ggcgatccag tggccgacca ccacggctgc
     4201 gatagcgagc acccgaatga ggtcgaggta agggttgcgc tgctcgtcgg tggcggcctc
     4261 gagacccatg cgtccagggt aggtgtgctt ggcgacgcgc tgacaagaaa tgtgaagccg
     4321 gggcgggctc cctggtgaga agcccgcccc ggcccgcacg cgtcacgccc cccgacgtga
     4381 cacgtcggcg gcctagctct ggttcagcgc ggcgctggct tgagtcgcga tcagtgcgct
     4441 cagcgtcagg gttccaccga acaggaacgc ctgcgtgagg ttggcgtggt tctgaccgat
     4501 gtagaggccg tcggcggatt gcagcgtgag cggcccggtg agcaacaccg gcccaccgac
     4561 cattcccatg aaagctccgc ccgcgagcgc gtcagggaag ttcgcgccgg tggcgatgcc
     4621 gacaagcgtc gggctggtga agaactccga ggcaaccaaa gcggcggttg catagcgatc
     4681 cccgccgacg atcggagtcg cgctcgggtc ggcgacgtgc gccgcctgac cgaccgcgta
     4741 gaccgtcggg ctcgtcagtc cggcgaggta ggtgtccacc gacgccggca tcacagttgc
     4801 ggccgtcaac aacaccgagc cgcccttcga tgctgcggct ggccctgctg acaatgcgtc
     4861 ggcaaagatg tcgccggttg ccaggaacac cgtgctcggc gatccttcac attgcgctac
     4921 gtcgacagac gttgcgaacc ggtcggcacc ggcgacgcgc acgacattga agcctgcggt
     4981 gttgagctgc gtgtcgatct cttgtgcaat cgctgcgtag ccgccgatca cgtagaccgc
     5041 gttagcgggt gcgggcgtgg tgcagccggt cgtcgtggtg cctggagtcg ttgctggcac
     5101 gggcatgact cgctcgattt cggtcagagt ggcgctgtcg agctgccccg gactcgtcag
     5161 aagcaagggc gccgtgaaca gcttgccgag ggggccggcg ctcatggcat cggcgaacgt
     5221 gtacgcggcg gtcaggacca cgtttttggc ggagtgggca gcggggaatt cctgcgtcga
     5281 gatggcgatg ctggtgccga tgcggtcggt gccggcgagt ctggtcaccg gcttcgtggt
     5341 ggtcgcgaac gcaggcacag cggcgatgcc aatcgcggta actccagcga tggcagccac
     5401 ggcgacgcgt cggacgatgg gtgtgaacat gcgatcctcc ttcagctgct caggtatcgg
     5461 gttgctgggt atctgacatg cccattcaac ggctcgctcg gtcagcacgg ggtcagcgaa
     5521 atgtaaggaa acggccaact ccgactacgt cgtgggtcgg gccgatcgcg ccttcttgac
     5581 ctgggtaacc gccatcggcg cggtcctcgt ccgggtagac gcacgcacca cgaggtcggg
     5641 ctcgaattcg atttgccggt gcacgtggcc cgcaggattt tgggtttcct cgagtagtaa
     5701 ctctgcggcg gccttgccaa ggaggtgtcg cggttggcgg accgacgtca gaggtaccgc
     5761 ggcggcgcct gcaaactcga tgtcgtcgta gccgacgatc gccacgtcgt tgggcacgtt
     5821 gagtccctgg cggatcagtt cttgcaggag gccaagcgcg agcagatcgt tcgcgcagaa
     5881 cacggcggtc ggacgccggt tggcggggag tccgccgagt ctttcccctg cttctcgccc
     5941 ggccccgacg gtcatcgaag cggtgtcgat gcacagcagc gagtccgggt cgcgacccgc
     6001 gcggctgagt gcgttgcgcg cgcccgctct ccggtctttc acctgggcga tcgtggtcgg
     6061 accgcccacg aacgccacac gttcgtggcc gatctcaagc aagtgggtca acgcgagttc
     6121 gccgccgctt acgtcgtcga cagagacgga gcacatgccg tcgccttcgg cgcggcggtc
     6181 gagaagcact accggcatgc cacgctcgac gaggcgggcc agccggaccg ggtcggcgtc
     6241 gacgggcgtg agcaagagtc cttgcacgcg ctgttcggcc atcaggttga ggtacgacgt
     6301 ttcctttgcg gcgtcctgcc cgctgttaca gaggatcacc gcgagacctg cctttgcggc
     6361 tgcctcttcg gcgccgcgag agacgtcggc gaagaacggg ttggcggcat cgaggaccag
     6421 gagtccgatg gtgcgactgc gaccggcccg tagctgccgc gccggctcgt tgcgcacgaa
     6481 cccgagcgcc ttgattgcgg tctggaccct gttgcgggtg tcctcggcta cccgctccgg
     6541 tcggttcagc acgttcgaga cggtcccgac cgagaccccg gcatgggcgg cgacatcctt
     6601 gatgctggcc gcggaagtca ccgggagctc acaggggact cacagggcac tcacgcggcg
     6661 ccacattact ttgaaacgac tcaactgtcg tcccctctcg ccccgacgtc ttccaacctg
     6721 gcccggcagc tgcctcgtca cgtcttggta acggtcccgg aattcctttg acatggctcg
     6781 gaagcccgct ctaacttcgc tctacagccg atattgaaac gattcatcct agccgagtca
     6841 agagcgacga gcggagggct gccatgaccg cgaccgacac gaccggcgtc ccgtcttcgc
     6901 gtgagacgcc gctgctcgag ctggcccacg ccaccaagtc gttcggcgcg gtgaaggctc
     6961 tcgaggacgg ctcggtgcgc ctgtacgccg gagaagccca cgcccttgtg ggcgagaacg
     7021 gggccggcaa gtcaacgctg gtcaagatcc tggcgggggt gcaccatccc gacagcggcg
     7081 aactgctcat cggtggcgag cccatcgtgt tgaacggtcc ggcagcttcg cgagccgcgg
     7141 gtgtctcgat catttatcag gaaccaaccc tgtttcccga cctcaccgtc gcggagaaca
     7201 tcttcatggg ccggcagccg atcgggcggg gccgccgcat cgacaaggcg gcgatgtcgc
     7261 gtgcggcgcg cgaggtcttc gctcggctcg gcgtcgagct aaacccgggc cgagtcgccc
     7321 gcgggctctc tgtcgcagat cagcagatcg tcgagatcgc gaaggccatc tcgttcaacg
     7381 cgcggatcat cgtgatggac gaacccaccg ccgccctgac ctcggtcgag gtggcgcggc
     7441 tgttcgatgt cgtccgcacg ttgcgcgccc aggggtcggc ggtgctgttc atcacgcacc
     7501 ggctcgagga agcgtttgcg atctgccagc gagtcacgat catgcgcgac ggtcggttcg
     7561 tcaggactgc cccaatcgag gacgtcacga tcgacgacat cattcgttcg atggtcggtc
     7621 gcgatctcga ccagctgttt ccgaagagcg agaccgctcc cggtgaggtc gttctcgcgg
     7681 tcgagcggct gacgcgcgaa ggcgtcttca ccgacgtgtc gttcagcgtg cgccggggcg
     7741 agatcgttgc cctcgcgggt ctggtgggcg caggtcgaag cgaggtcgcc agggccatct
     7801 tcggcatcga ccgccgcgat gcggggcggg tgctggttcg tggccaggaa ctgccgaatg
     7861 gctcgccgcg agctgcgatg gcagccggca tcgcgctggt gcctgaagat cgtcgtcagc
     7921 aggggctcgt gatggatctc ggcatcgacc agaacgtgtc gctggcctcc ctgtcgagcc
     7981 tcgagcgatt cgggctgatc cgccggtcga gtgagcgcgc actcgcggca gagtgggcgc
     8041 tcaagctgca actcaagtac agccggttgc gcaatcccgc ctcgacactg tcggggggca
     8101 accagcaaaa gaccgtgctc gggaagtggc ttgcgcgtca cccgacgatc ctgatcatcg
     8161 acgagccgac ccgcggtatc gatgtcggca cgaaagccga ggtgcaccgc ctgctcgacc
     8221 agttggtcgc cgacggtgtt gcggtgctga tgatctcctc ggagctaccc gaggtgctcg
     8281 gcatggccga tcgggtgctc gtcctgcgcg aaggccggct cgtggccgag ctgtcgcgag
     8341 ctgacgccga cgaggcttcg atcatgcgcg ccgccaccgg acaaagcgtc gggtcggcag
     8401 cttgagcgtc atggcgacgg ctccgccgtc ggtcacgaag gtcggccggg ccgaatcgct
     8461 gctacgcgca cgggagttgg gcatcggcat cgcgatcgtc atcgtctttg gtttgacgac
     8521 tctgaagaat ccggcattcg ctcatgcact gagcgtgcag cagttgctca caggcgcgtc
     8581 gttgatcgcg ctgctcgccg tcggcgaaac gcttgtcatt atcacgcgca acgtcgatct
     8641 gtccgttggc tcggtgcttg ggctctcggc ctacatcgtc ggcaatacgt tcgcccatca
     8701 cccgcacact gcaccgatcg tcggcttcgc gatcggcatc gtcgtcggtg cggtcttcgg
     8761 cgcgatcaac gggatcatcg tcacggtgtt gcgggtgccc agcttggtcg tcacgctggc
     8821 gaccctgtac atcatccgcg ggctcgacgc gatgatcgtc aacggcaaac agatcgatcc
     8881 gtcgagcgtg cccaaggcgt ttcaggcggt cgggaaccgc aacatcttcg gcgtcccgtg
     8941 gctggccatc atcgtggcga tcgtcattgc gatcgccgga tacgcgatgc gctcgttccg
     9001 cgcgagtcgt gacatctacg cgatcggctc caaccccgaa gccgccgcgc tggcaggtgt
     9061 gcccatcggt cggcgggtat tcacggcgtt cctcatcagc ggagcactcg ccggtctggc
     9121 aggagcactg ttcctttccg aggtggcgac ggtcgatgcc accggaggca ccgggtacga
     9181 actactcgtg gtgtcggcgg tggtggtcgg tggtgtcgcg atctttggcg gtagcggcac
     9241 ggtcctcggc gcagcgctgg gcgcgctgtt gctcaacacg atcaatcagg cgcttggcgc
     9301 gttgaagctg tcgtcgtttt gggatcaggc agtagcgggt ggcctgctgc tagccgcgat
     9361 cgcgttcgac cgatggctcg gtctgcgcat cgccaagcgg atgaagtcgg cacgaggtga
     9421 gcatcgtgtc gtctgatctc gcaaccgcgc cgccgcgggc tcaacacgtc gacaccgggc
     9481 tgccgtcgtg gcgctggctg ctgcgctggg aaacggcgct tgtcttcatc ttcgtcggca
     9541 ccctgttctt cggtgcgtcg gagtcgccgc acttcatgac gcataccaac gtcttcttca
     9601 tcgggctcaa catgggcgag atcgcgatca tggcgctccc gctgacgctg atcgtcatca
     9661 ccggcgagat cgatctgtcg gtggcggcga tgctcggcct ttcgagcacc atgttgggct
     9721 acctgtacga gcaccattgg ccgatcggct tggcgatggt ggcggtgttg gtcatcggtg
     9781 ccgttggagg cgcgttcaac gggttcctgg tgaccaagct cggactgccg tcgatcgccg
     9841 tgaccatcgg cacattggcg ttgttccgtg gcatcgcgga aatcgtgctc ggcgccaact
     9901 ccgtggtgaa gttcccgacc ggtctcacca atatcggcgt caagccggtg ccgcacacgg
     9961 agttgtcgta ttccgccgtg atcttcatcg tgctcgcgat cgtctacgga gtgatcctcc
    10021 atgcgacgcc cgcaggtcga tcgatgatcg cgatcgggct gcaatcggag gcggctttct
    10081 tcgccggcat cagagtgaag cggatcaagt tcatcctcta cacaatgagc ggcgtcgtct
    10141 gcgccttcgc tgggatcttg tggacctatc ggctgtcgtc gtcgcgctac gacaacggcc
    10201 tgggcctcga gctcgacgtc gtggcgatcg tgttgttcgg cggcgtgtcg attttcggcg
    10261 gccgcggctc gatcatcggc gtcgtgctcg cggtcatcgt gttcggctcc atcaaagaag
    10321 cactcacctt gatgaacgtg tccgcccaag cacagaacat cgtcacgggc ggactgctcc
    10381 tgatcagcgt catcctcccc aacgtcgcca gcagcgtgcg gcgagttcga gctagagccg
    10441 gcctccgcac ctagaggccg gtgcgggttt tacgaacgga acatccctgc gagtcgagtg
    10501 aaacgattca aaatcagtcc cgaacaccca ccacagagga gaccaaaaat gattcacggt
    10561 tctcgagctc gccgtctcag cgcggtgacg ttcgcggtag cgctcgtgac gttggctgcg
    10621 gcgtgtagta gttccaagaa ggcggctccc gctgcgtcgt cagctgcgcc tacgacctct
    10681 gccgcagctc cggcgacgtc ggccgctccg gccacgacgt cggctgctgc ccccgcaacc
    10741 tccgccgctg ccgcgccgac gtctgccgca gccggctcag gagctccggc aaacatcaag
    10801 aagggactgg tcgtctactt cattccgaag gacacccaga acccctacga agtcatcgct
    10861 gacaagggtg gcgcggccgc gctcgccgag ctcggtggga aggtcgtcgt gagcagtggc
    10921 acggctgaca ccgcagctgc gcagatcccg tcgatccagg cggcgatcc
//
LOCUS       DASWVF010000122         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_130898, whole genome shotgun sequence.
ACCESSION   DASWVF010000122 DASWVF010000000
VERSION     DASWVF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130898"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VGD55_02165"
     CDS             <1..687
                     /locus_tag="VGD55_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869177.1"
                     /translation="MAICLPAAAPAPLAGADALVGATVTANKSSSPSSYSAASFPLVH
                     LGATAKRCTYIVGYQVNESVLVSATGAPNVAARAAVLAVIKAAVADLSSRTGQPYRYD
                     GTTTFAPTLTNYSTGPDDLVIVIKGSANNSFYMAAAPGTYLAGMTRWLVGGSGTSVVE
                     ISPLMIDLRTAASRAYLYETVQHELAHSVGLPHSKSTADVMFAQLSTATPAVYSKADT
                     AALAASACRS"
     gene            755..>1889
                     /locus_tag="VGD55_02170"
     CDS             755..>1889
                     /locus_tag="VGD55_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0869178.1"
                     /translation="MSAQTEELLADLNPQQRAAVVHEGGPLLIVAGAGSGKTRVLTRR
                     IAYLLAHRHVSPTEILAITFTNKAAGEMKERVAAMVGNRAKVMWVMTFHSACVRILRE
                     NAKRLGFTTTFSIYDSADSHRLMTLVCRELDLDPKRYPPRTFNAQVSNLKNELIDPED
                     YERKANNSHEKVLAEAYTAYQRRLREANAFDFDDLIMVTVNLFQAFPDVAEHYRRRFR
                     HVLVDEYQDTNHAQYTLVRELVGGAVSQQEKRTVDGDLVRAASSDVPPAELVVVGDAD
                     QSIYAFRGATIRNILEFERDYPNATVVLLEQNYRSTQTILSAANAVITRNEGRKAKNL
                     WSNAGDGEQIGGYVSDDERGEATFVAEEIDRLHDLGLAAYSDVA"
BASE COUNT          344 a          635 c          580 g          330 t
ORIGIN      
        1 atggcgatct gtcttcccgc cgccgcaccg gcacctctgg ctggcgccga cgcgctcgtc
       61 ggcgcgacag tgactgccaa caagtcctcg agcccatcga gctactcggc tgcgagcttt
      121 cccctggtgc accttggtgc caccgccaag cgctgcacct acatcgtggg atatcaggtg
      181 aacgagtcgg tgctggtgtc tgcaaccggc gcaccgaatg tggccgcccg tgccgcggtg
      241 cttgcggtta tcaaggcggc cgtggccgac ctcagctcgc gtaccgggca gccataccgc
      301 tacgacggca ccaccacgtt cgcgccgacc ttgaccaact acagcaccgg acccgacgac
      361 ctggtcatcg tgatcaaggg gtcggcgaac aacagcttct atatggccgc cgcgcccggg
      421 acctacctcg caggtatgac ccggtggctc gtcgggggta gtggcaccag cgtcgtagag
      481 atctcccctt tgatgatcga cttgcggact gccgcgtcca gggcgtactt gtacgagacc
      541 gttcagcacg agctggcgca ctcggtaggg ctgccgcatt ccaagagcac ggcggatgtg
      601 atgtttgctc agctcagcac cgctactccc gccgtctact cgaaggcgga cacggcggcg
      661 cttgcggctt ctgcctgccg ctcctgaccg cacccgatct gcaccctcct tcgccggtcc
      721 tcgtttgtcg gtccgctccg tagactgaac ggcgatgtca gcgcagaccg aagagctcct
      781 tgccgatctc aacccccagc agcgcgcggc cgtcgtgcac gaggggggtc cgctgctcat
      841 cgtcgccggt gccggctccg gaaagacgcg ggtcctgacc cgccgcatcg cttacttgct
      901 cgcgcaccgg cacgtctcgc caaccgagat tctggcgatc acgttcacca acaaggccgc
      961 cggtgagatg aaagaacgcg ttgccgcgat ggtgggcaac cgggcgaagg tcatgtgggt
     1021 gatgacattc cactcggcct gcgtgcggat cctgcgcgag aacgccaagc ggctcggatt
     1081 caccacgacg ttctcgatct atgactccgc cgactcgcac cggttgatga cgctggtgtg
     1141 ccgcgagctc gacctcgacc cgaagcgcta cccgccgcgc accttcaacg cgcaggtctc
     1201 gaacctgaag aacgaactca tcgacccaga ggactacgag cgcaaggcga acaacagcca
     1261 cgagaaggtc ctcgcggagg cgtacaccgc ctaccagcga cggctgcgtg aggcgaacgc
     1321 gttcgacttc gacgacctga tcatggtgac cgtcaatctc tttcaagcgt tccccgacgt
     1381 cgccgagcac tatcgccgtc gcttccggca cgtgctggtc gacgagtacc aagacacgaa
     1441 tcacgcgcaa tacacgctgg tccgtgagtt ggtcggcggc gctgtgtcgc agcaggagaa
     1501 gcgcacggtc gacggtgatc tcgtaagagc ggcgtcctcc gacgtcccgc ccgcagaact
     1561 tgtcgttgtt ggtgatgccg accagtcgat ttacgcgttc cggggcgcga cgatccgcaa
     1621 catcttggag ttcgagcgcg actacccgaa cgcgaccgtg gtgttgctcg agcagaacta
     1681 tcgctcgaca cagacgatcc tgagcgcagc caatgcggtc atcacccgca acgagggccg
     1741 caaggcgaag aacctgtggt ctaacgccgg tgacggcgaa cagatcggtg gttacgtttc
     1801 cgacgacgag cgcggtgagg caaccttcgt ggctgaggag atcgaccgcc tgcacgatct
     1861 cggcctcgcg gcctacagcg atgtggcga
//
LOCUS       DASWVF010000123         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_105399, whole genome shotgun sequence.
ACCESSION   DASWVF010000123 DASWVF010000000
VERSION     DASWVF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105399"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VGD55_02175"
     CDS             complement(<1..1232)
                     /locus_tag="VGD55_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0869179.1"
                     /translation="MTGVPAEQADGGEAVVQACRDAGFDHIFSSPGSEWAPVWEALVR
                     QRESGSPGPTYFDLTHETLAVGMATGYTSVTGKPQLVLLHAGAGLLQGANAIHGALLT
                     GVGVVVCSGESNGYGDAAGRDPGSQWYRNLSMVGGPQAIAATFTKWSSQVGDVAALHP
                     MIGRARDIASQVPAGPVYLNTPVEVLLQPWKPSSRVGVSRAVAGKAVADPADVDRVAQ
                     VLASATQPVIAVESVGRDPAAWEALVELAELLGIPVVEPQSAVCANFPRTHELHQGSD
                     LERLAAGADVVVLLGCRAPWYPPSNKSGKATVVVIDEVPQRVHMDYQVLTADDYVGGD
                     AALTLRALTERVRTRGVDALAVNRRRTEFAERFREDAAERVAQEQNSFGSDGAVDPVA
                     LVASLREAIGPAAVVVDET"
     gene            complement(1235..2101)
                     /locus_tag="VGD55_02180"
     CDS             complement(1235..2101)
                     /locus_tag="VGD55_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0869180.1"
                     /translation="MFIVDSQVHIWREESPDRPWVPGARERLKLNGHRDEAFSYNECI
                     ELMDEAGIDRALILPPSWEGDRTDYALEACEVYPERFGVMARIPQNKPAEGTAMMRDF
                     AQNPYIKGTRLTFHRPIDRNWMIDGTNDWYWPLAEELGIKTMVHAPIWKTELGAIAER
                     HPQLRIIIDHMGIMARCVDDAIGYWVEETAALAKYPNIYVKVSSIPGYSSQPFPNLNI
                     EQYVRMMVDKMGPQRCFWGTDLTRLLGHGLTYQDTIEQFTKYFSFTEEELEWIMGRGI
                     CECLDWPIPVKA"
BASE COUNT          412 a          728 c          656 g          416 t
ORIGIN      
        1 gtctcatcga ccacgacagc ggccggccca atcgcctcgc gtaacgacgc cacgagtgcg
       61 accgggtcga cagcgccatc ggagccgaac gagttctgtt cttgcgcgac ccgttccgca
      121 gcgtcctcgc gaaaacgttc cgcgaactcc gttcggcggc ggttgacagc caatgcatcg
      181 acgccacgcg tgcgcacgcg ttctgtgagc gcgcgcagcg tcagagcagc gtcgccgccc
      241 acgtagtcgt cagcggtcaa cacctggtag tccatatgca cccgctgtgg cacctcatca
      301 atcacgacga ccgtggcttt gccggatttg ttgctcggcg ggtaccacgg agcgcggcag
      361 cccaggagca cgacgacgtc ggcacctgcg gccagtcgct cgaggtcgct gccctgatgc
      421 aactcatgcg tgcgcgggaa atttgcacac accgccgact gcggttcgac caccgggatt
      481 cctagtagtt ctgcgagttc gaccaacgct tcccacgcgg cgggatcacg cccgacggat
      541 tcgacagcga tcaccggctg ggtagcggat gcgagcacct gcgcgacccg atcgacgtcc
      601 gctggatctg caaccgcctt ccctgcgaca gcgcgcgaca ccccgacccg cgatgacggt
      661 ttccacggct gtaacagcac ctcaacggga gtgttcagat agaccgggcc ggccggtacc
      721 tgcgacgcaa tgtcacgcgc gcgcccaatc atcgggtgta gggccgcgac atcgccaact
      781 tgcgacgacc atttcgtgaa cgtggcggcg atcgcttggg ggccgccgac catcgacagg
      841 ttgcgatacc actgtgaacc agggtcacgg cccgctgcgt cgccataccc gttcgattcg
      901 cccgaacaca ccacgacacc gacgccggta agtagggctc cgtggatcgc gtttgcgcct
      961 tggagcagcc cggcgccggc gtgcagcaac acgagttgag gcttgccggt caccgacgtg
     1021 taaccggttg ccatccctac ggcgagagtc tcgtgagtga ggtcgaagta cgtcggcccg
     1081 ggcgagccac tctccctttg acgcacgagt gcttcccaga ctggggccca ctccgaacca
     1141 ggcgaggaga agatgtggtc gaacccggcg tcacggcagg cttgtactac tgcctcgcca
     1201 ccatcagcct gttcagctgg cactccggtc actctcacgc cttcacaggg atcggccaat
     1261 caaggcactc acaaatcccc ctacccataa tccactcgag ttcctcctcg gtgaaactga
     1321 agtacttggt gaactgctcg atggtgtctt gataggtcag accgtggccg agcaatcggg
     1381 tcagatccgt tccccagaag cagcgctgcg gacccatctt gtcgaccatc atgcgcacgt
     1441 attgctcaat gttcaggttc ggaaatggct gcgaagagta gccaggaatg ctcgacacct
     1501 tcacatagat gtttgggtac ttcgctagcg cagccgtctc ttcgacccag tagccgatcg
     1561 cgtcgtcgac gcacctggcc atgatgccca tgtggtcgat gatgatgcgc aactgcggat
     1621 gacgctcggc gatcgcaccg agctcggtct tccagatcgg ggcgtgcacc atcgtcttga
     1681 tgccgagttc ttctgccagc ggccaatacc agtcgttcgt tccgtcgatc atccagttgc
     1741 gatcgatcgg gcggtggaac gtgagccgag tgcccttgat gtacgggttc tgtgcgaagt
     1801 cgcgcatcat tgcggtgccc tcggccggct tgttctgcgg gatccgagcc atcacaccga
     1861 acctctccgg atagacctcg caggcttcaa gtgcgtagtc ggtgcggtca ccctcccacg
     1921 atggcggcag gatgagcgcc ctgtcgatac ccgcctcgtc catgagctca atgcactcgt
     1981 tgtaactgaa tgcctcgtca cggtgaccgt tgagcttcag gcgctcacgc gcgcccggca
     2041 cccacggacg gtctggcgac tcttcacgcc agatatgaac ctgtgagtca acgatgaaca
     2101 cgattacctt cccgatccag ttggctgcaa ttcggcgacg atgtgttctg caatcgcaag
     2161 gcaagatgtg gccgcgggcg acggagcgtt acgcacggcg atgaccgcgc cg
//
LOCUS       DASWVF010000124         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_385691, whole genome shotgun sequence.
ACCESSION   DASWVF010000124 DASWVF010000000
VERSION     DASWVF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385691"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="modB"
                     /locus_tag="VGD55_02185"
     CDS             <1..607
                     /gene="modB"
                     /locus_tag="VGD55_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY0869181.1"
                     /translation="ATVIAIVLGVPIAWVLARLEFRGRALLRALITLPLVLPPVAGGI
                     ALLLAYGRRGVVGSSLLKVFGFSLPFTTAAVVVAETFVAMPFLIVSVEGSLRGLDARY
                     DQAAATLGASRLFTFRYVTLPMLAPGIAAGAVLCWARALGEFGATITFAGSYPGTTQT
                     MPLQVYLALENNPDAAIALSLVLLVVSVVILAGLRDRWLRA"
     gene            604..1665
                     /locus_tag="VGD55_02190"
     CDS             604..1665
                     /locus_tag="VGD55_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869182.1"
                     /translation="MTDLDAHIIVERDSWRLDVEIEVAAETVLALLGPNGAGKSTALR
                     VIAGLLPASAGHVRVGGETWDDPAAGVRLATEQRSLGVVFQDYLLFPTMTARDNVAFG
                     LRAKGIDKTTARDQADAWLTRVGLHDEAGRRPAQLSGGQTQRVALARALATTPELLLL
                     DEPLAALDAGTRMNVRGDLRHHLADFKGAAVLVTHDPLDALILADRVAVIENGAVVQT
                     GTPQEITRHPRTRYVAQLVGLNLLGGQANGTQVTLVDATDLITSEPHVGEVFVVARPT
                     SISVHRDVPHGSARNVWKGIITTVEQHGDLVRLFVTGPPDVVVDVTTAAMADLRLGVA
                     EAVWLSVKATDLTVYDAGA"
     gene            complement(1671..1826)
                     /locus_tag="VGD55_02195"
     CDS             complement(1671..1826)
                     /locus_tag="VGD55_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869183.1"
                     /translation="MQNADINTLGFVIVGLFVVTWIAALLIWRVGHIEEKWNVGMRKN
                     ALAEPAD"
     gene            complement(1887..2810)
                     /locus_tag="VGD55_02200"
     CDS             complement(1887..2810)
                     /locus_tag="VGD55_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY0869184.1"
                     /translation="MSTHLEVRPVGTRIARIRQGLTPGEWTKLAGMFAVIIALNVVGW
                     FIVIALVAPHHYSLGTKTFGIGIGLTAYTLGMRHAFDADHIAAIDNTTRKLMNEGKRP
                     MSVGFWFSLGHSSIVFALALLLSLGVKALVGPVKNDNSALHHYTGLIGTSVSGTFLFV
                     IAAINLVILVGIVKVFRDMRRGVYDEETLEEHLANRGLMNRILRPIMRSIKRPGQMYG
                     VGLLFGLGFDTATEVSLLVLAAAGAASALPWYAILSLPVLFAGGMCLFDTIDGTFMNF
                     AYGWAFSKPIRKVYYNITITSLSVMVAFVIG"
     gene            3071..3526
                     /locus_tag="VGD55_02205"
     CDS             3071..3526
                     /locus_tag="VGD55_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0869185.1"
                     /translation="MAARTHDADRLSAAVGELRAQGTRITAARVAILEVLSASEAHLS
                     ADDVAQAVNALQPRVHRATVYRTLDSLVAAGLVAHTHLGHGTAVYHLTTTPHAHCQCQ
                     RCEAIIDVPRSVLRTVSSRLLDDYGFSLDTGHSALLGLCKQCAETPLLS"
     gene            complement(3519..4358)
                     /locus_tag="VGD55_02210"
     CDS             complement(3519..4358)
                     /locus_tag="VGD55_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY0869186.1"
                     /translation="MIATLVIFLREGVEASMIVAILLAYLDRTGKRQYFRDVFIGVGA
                     ALVLAGAGGVIAYHTVKTYDGSRAQTIFETFTYLLAAAVLTYMTFWMRTHARGLSAEL
                     RARTDAAISKGARTGMALIAFQAVGREGLETVVFTLAILFSSSHTGGALPGALIGLAI
                     ALAIAFVIYKLGHRLNLSKFFTIIGALLMVFAAGLLVDAVQNMQELGWLPGAGVQLWH
                     TSGVLSEDTTVGDMLHSFFGYADSPTALQAAVYVLYLAVSLLSFFGVWRKIRPRPAAA
                     AVS"
     gene            complement(4396..>5032)
                     /locus_tag="VGD55_02215"
     CDS             complement(4396..>5032)
                     /locus_tag="VGD55_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869187.1"
                     /translation="GSALAIGGVLLANAIAGGQGARRRFGVQLSGMWGYRDLFLLGAA
                     FLLMETRSVTSFALLFGTTWVVNALVFGGVLVAVLAAVEFTRHFPTPPIQIMYGLLIA
                     GLALNWFVPTSWLLSLGFFVRLVVAVFIAFLPIFAANIIFAKRFSETGDAALSFGTNL
                     LGAMLGGCLEYLSLVFGYHALLILAAVLYVGAYLLMPSQARVDTETLVRLT"
BASE COUNT          936 a         1655 c         1618 g          823 t
ORIGIN      
        1 ggcgacggtc atcgccatcg tgctcggcgt cccgatcgcg tgggtattgg caaggctcga
       61 attccggggt cgagccttgc tgcgagcgct gatcacgctg ccgctcgtgc taccgccggt
      121 tgccggcgga atcgcgctgc tccttgccta cggccgtcgc ggcgtcgtcg gtagctcgct
      181 gcttaaagtg ttcgggtttt cgctgccgtt caccactgcc gccgtcgtcg tggcggaaac
      241 atttgtcgcg atgccgtttc tgatcgtgtc ggtcgagggt tcgctgcgcg gcctcgatgc
      301 acgctacgac caggcggccg cgacccttgg cgcgtcgcgg ctcttcacct tccgatacgt
      361 gacgctgccg atgctcgctc ctggcatcgc agcgggcgcg gtgctgtgtt gggcgagggc
      421 gctcggggag ttcggcgcga cgatcacctt cgcgggcagc tacccgggca ccacgcaaac
      481 catgccgctc caggtctacc tcgcgctgga gaacaaccct gatgccgcga tcgcgctgag
      541 cctggtactg ctcgtcgtct ccgtcgtgat cctcgccggc ctgcgcgacc gctggcttcg
      601 agcgtgacgg acctcgacgc acacatcatc gtcgagcgcg actcatggcg actcgacgtc
      661 gagatcgagg ttgccgccga aacggtgctg gcgctgctcg gacccaatgg tgctggtaaa
      721 tcgacggcgt tgcgcgtcat cgccgggttg ctgccggcgt ccgccggtca cgtgcgtgtc
      781 ggcggcgaaa cgtgggacga ccccgccgcc ggcgtccgac ttgcgacaga gcaacgctcg
      841 ctcggcgtcg tgttccagga ttacctgctc ttcccgacga tgaccgcgcg cgacaacgtg
      901 gcgttcggcc tgcgcgccaa gggcatcgac aagacgacgg cgcgcgatca agccgacgcg
      961 tggcttacca gggtgggttt gcacgacgag gccggacgcc gacctgcgca gctttctggc
     1021 gggcagaccc agcgagtggc gctggcgaga gccctcgcca caactccgga gctgctgctt
     1081 ctcgacgaac cactcgccgc actcgacgcc ggcacccgca tgaacgtgcg cggcgatctg
     1141 cggcaccatc ttgccgactt caagggcgcc gccgtgctcg tcacgcacga tccgctcgac
     1201 gcgctgatcc tggccgatcg ggtcgcggtg atcgagaacg gcgccgtcgt gcaaaccggg
     1261 acgcctcaag agatcacccg gcacccgcgc acccgttatg tggcacagct ggtcgggctg
     1321 aacctgctgg gtggccaggc aaatggcacc caggtcacgc tggtcgacgc gactgacctg
     1381 atcacgtcgg agccgcacgt cggtgaggtg ttcgtggtcg cgcggcctac gagcatttcg
     1441 gtacaccgcg acgtcccgca tggaagcgca cgaaacgtct ggaagggcat catcaccacg
     1501 gtcgagcagc acggcgacct ggtgcggttg ttcgtgaccg gcccgcccga cgtcgtcgtc
     1561 gatgtcacga cggccgcgat ggccgacctg cgactcggcg tcgccgaagc tgtgtggctg
     1621 tcggtcaaag cgaccgacct cacggtgtac gacgccggag cttgacccgg ttagtcggcg
     1681 ggctccgcga gggcgttctt gcgcatgccg acgttccact tctcttcgat gtgaccgacg
     1741 cgccagatca gcagggcggc gatccaggtc acgacgaaga gcccgacgat gacgaagccg
     1801 agcgtattga tgtcggcatt ttgcatggcg gtccagaacc agcccgtgat gttgagctgc
     1861 tgcgagagca gcccgagaag ctcgactcaa ccaatgacga acgcgaccat cacagaaagc
     1921 gacgtgatgg tgatgttgta gtagaccttg cggatcggct tggagaacgc ccagccgtag
     1981 gcgaagttca tgaaggtgcc gtcgatcgtg tcgaacaggc acatgccgcc ggcgaaaaga
     2041 accggcagcg acaagatcgc gtaccacggc aacgcgctgg ccgcccccgc tgcggccaac
     2101 accagcagcg aaacctcggt cgccgtgtcg aacccgagcc cgaacagcag cccgactccg
     2161 tacatctggc ccggtcgctt gatcgacctc atgatcggtc gcaggatccg gttcatcagg
     2221 cctcggttag cgaggtgctc ctcaagggtt tcctcgtcat agacaccgcg acgcatgtcg
     2281 cgaaagacct tgacgatgcc gaccagaatc accaggttga tcgccgcgat cacgaacaag
     2341 aaagtgcccg acacgctcgt gccgatcagg ccggtgtagt ggtgcagggc tgagttgtca
     2401 ttcttgacgg gtccgacgag cgccttcacg ccaagtgaga gcagcagggc caacgcgaac
     2461 acgatgctcg agtggccgag cgagaaccag aatccaaccg acatcggccg cttgccctcg
     2521 ttcatcagct tgcgggtggt gttgtcgatc gcagcgatgt ggtcggcgtc gaaggcgtgt
     2581 cgcatgccaa gggtgtaggc ggtcaagccg atgccgatcc cgaacgtctt ggtgcccagg
     2641 ctgtagtggt gcggagcgac cagtgcgatg acgatgaacc agccgacgac gttgagggcg
     2701 atgatcactg cgaacatccc ggcgagtttg gtccactccc cgggggtgag gccctgccgg
     2761 atgcgtgcga ttctggtgcc tacgggccgg acctcaagat gggtgctcat cgcgactccc
     2821 ctcgtcgatc tgcccggccc ggttgatcac cggaccggcc tggtgcgctc agatgcgact
     2881 cattcgcagg ccaacccagg ttcggattgg tgccgttcgc gaatctgtcg caggcggggc
     2941 aactcccctg cctcacagta tcctggctgt tgcgactatg tcgcaacgca ctttcccgtg
     3001 gagttggccg cgcatgggtg gcccgcgcgg ctacgctgca atcgcccgag actcaagcga
     3061 ggagggatcc gtggcggcca gaacgcatga cgccgatcgg ctctccgccg ccgtcggtga
     3121 gttgcgcgct cagggcactc ggatcaccgc ggcgagggtg gcgatcctcg aggtgctgtc
     3181 ggcgtctgag gcgcacctgt cggccgacga cgtcgcccag gccgtcaacg cgctgcagcc
     3241 tcgagtgcac cgagccaccg tgtatcgcac gctggattcc ctcgtcgccg ccggcctggt
     3301 cgcccacact cacctcggcc acggcaccgc ggtctatcac ctgacgacga ccccacacgc
     3361 ccactgccag tgccagcgct gcgaagcgat catcgacgtc ccgcgaagcg tgctacgaac
     3421 agtcagcagc cgactgctcg acgactacgg cttcagtctc gacaccgggc acagtgcact
     3481 gctggggctg tgcaagcagt gtgccgagac ccctttgctt agctgactgc tgcggccgcc
     3541 ggccggggtc ggatcttgcg ccacaccccg aagaacgaga gcaacgagac cgcgaggtac
     3601 agcacgtaaa ccgcggcctg caacgcggtc gggctgtcgg cgtagccgaa gaaactgtgc
     3661 aacatgtcac cgaccgtggt gtcctcgctg aggacaccgc tcgtgtgcca caactgcacg
     3721 cccgcgccag gcagccagcc caactcctgc atgttctgca cagcgtcgac gagaagcccg
     3781 gcggcgaaga ccatgagcaa cgcgccgatg atcgtgaaga acttgctcag gttcagccgg
     3841 tggccgagct tatagatgac gaacgcgatc gccaacgcga tcgcgaggcc gatcagcgcg
     3901 cccggtagcg caccgccggt gtggctgctg gagaacaaga tcgcgagggt gaaaacgact
     3961 gtctccaagc cctccctgcc gaccgcttgg aacgcgatca acgccatgcc ggtgcgcgcc
     4021 cccttgctga tggcggcgtc agtgcgcgcc cgcaactcag ccgacaggcc gcgcgcatgg
     4081 gtgcgcatcc aaaacgtcat gtacgtcagg acggcggcgg cgagcaggta ggtgaacgtc
     4141 tcgaagatgg tctgcgcccg cgaaccgtcg taggtcttga cggtgtggta cgcgatgacc
     4201 ccacccgcac ccgcaagcac gagggcggcc ccgacgccga tgaagacatc gcggaagtat
     4261 tgccgcttgc cggtgcgatc gaggtaggcg agcaggatcg ccacgatcat gctggcttcc
     4321 acgccttcgc gcaggaagat taccaacgtc gcgatcacga acagcctcca ccgattcgac
     4381 cagccacacc tgaggttagg ttagcctaac caatgtttcg gtgtctactc gggcctggct
     4441 gggcatcagc aggtacgcgc cgacgtacag caccgccgcg agaatcaaaa gcgcgtggta
     4501 cccgaacacc agcgacaggt actcgaggca gccaccgagc atcgcgccga gcagattcgt
     4561 gccgaacgac agcgccgcat caccggtctc ggagaaccgt ttcgcgaaga tgatattcgc
     4621 ggcgaagatc ggcagaaacg cgatgaaaac cgcaacaacg aggcggacga agaatccgag
     4681 cgaaagcagc cacgacgtcg gcacgaacca gttgagggca aggccggcga tcagcaaccc
     4741 gtacatgatc tgaatcgggg gcgtcgggaa gtgccgggtg aactcgactg cagccaggac
     4801 ggcgacaagg acacccccga acaccagggc gttgactacc cacgtcgtcc cgaataaaag
     4861 cgcgaagctc gtcacgctac gtgtctccat taacaggaag gccgcgccta acaggaaaag
     4921 atcgcggtat ccccacatgc ctgagagttg cacgccaaaa cgtcgccgag cgccttgccc
     4981 accggcgatc gcattggcca gcagcacgcc gccgatcgcg agtgcgctgc cc
//
LOCUS       DASWVF010000125         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_436721, whole genome shotgun sequence.
ACCESSION   DASWVF010000125 DASWVF010000000
VERSION     DASWVF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436721"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGD55_02220"
     CDS             <1..521
                     /locus_tag="VGD55_02220"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0869188.1"
                     /translation="LASLAVTTMRNARRRGDGVVEQANTAATHLTAQFSGEFFVTLAL
                     LRIDVPSGAVAAVNAGHQLPILVRDGVVRTIELAATPPLGMFPDTSYVPERLQLQPGD
                     RLILISDGIVEVKPVGGNALGEQGLHDLLRRMIDLSAVETVRQLTAAVLAHRAGDLQD
                     DATAVCLDWRGA"
     gene            complement(616..1707)
                     /locus_tag="VGD55_02225"
     CDS             complement(616..1707)
                     /locus_tag="VGD55_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEY0869189.1"
                     /translation="MTAPSADPADTDEPDFRLSTPADIVEVVPYLVGFQPENSLVVLS
                     LRGERKRLGLTARVDLPAARSANACAREFVGYLKRDGADFAIVVLYPPSGGRSHPSVR
                     RIAAALTRQLDQARIAVVDVLCVFDGLWWSLHCTDADCCPPDGTPVDRDGTSVAAAVM
                     TVAGQVVLSSRKELERTLDPVRGAVRAAMEYALPRADLELADRVLAGNRVAVTAETIE
                     LFRVAVRERVATRAAPGSEDPNVGSIDHAARLIVGLEDVHARDELLTWFDGTWGDATR
                     DVLNDLARRTAPPFEVPTLTLFAWISYLQGNGALAGIALDRALAIEPDYRLAQLLDQA
                     LRAPLNPEGLRMLFSTAGGATLAAPEDPL"
     gene            complement(1808..2128)
                     /locus_tag="VGD55_02230"
     CDS             complement(1808..2128)
                     /locus_tag="VGD55_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEY0869190.1"
                     /translation="MPRAWSAKRERQYEHIRDGLEARGRLENLAEEIAARTVNKERAR
                     AGESPTASVSSIHDISSGRRGGLRSHRGPGGRTKEQLYAEARRKNVKGRSAMTKAQLE
                     RAVD"
     gene            complement(2128..2262)
                     /locus_tag="VGD55_02235"
     CDS             complement(2128..2262)
                     /locus_tag="VGD55_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869191.1"
                     /translation="MLLIVALVLFVLFAGLGFAAHVLWLGLILALILGLIHAFTRSSA
                     "
BASE COUNT          445 a          806 c          784 g          417 t
ORIGIN      
        1 tgctcgcaag cctcgccgtg acgacgatgc gcaatgcccg tcggcgcggc gatggcgtgg
       61 tcgaacaggc gaacacggcc gcgacccacc tcacggcgca attcagcggc gagtttttcg
      121 tcacacttgc gctgctgcgt atcgatgttc cgtcgggtgc ggtcgcggcg gttaatgcgg
      181 gtcaccagtt gccgattctg gtgcgcgatg gggtggttcg cacgatagaa cttgctgcga
      241 ccccacctct tggaatgttt cccgatacgt cgtacgtgcc ggaacggctt cagcttcaac
      301 ctggcgaccg gctcatcctg atcagcgacg ggatcgtcga ggtcaaaccg gtcggtggga
      361 acgcactggg cgaacagggc ttgcatgacc tgctgcgtcg catgatcgat ctgtctgcag
      421 tggaaaccgt ccgacagttg acagctgcgg tgcttgctca tcgagcgggc gacctgcaag
      481 acgacgcgac cgctgtctgc ctcgattggc gcggagcgta ggtcgcacct gcgccaaagg
      541 agcgcgctct actctcatca tcacagttct ggacaatagt gagcgtcggg atgatcttca
      601 cccgcgcgcc tggaactaga gaggatcctc gggcgcggcc agcgtggctc ccccggcggt
      661 agaaaacagc atcctgagcc cttccgggtt gagcggcgcg cgcagcgctt ggtcaaggag
      721 ctgcgccaat cggtagtcgg gctcgatggc gagcgcgcga tcgagtgcga tgcccgcgag
      781 cgcgccgttg ccctgcaggt agctgatcca tgcgaacagg gtcaatgtcg gaacctcaaa
      841 gggcggcgcc gttcgccgtg cgaggtcatt cagtacgtcg cgggtcgcat cgccccatgt
      901 gccgtcgaac cacgtcagca actcgtcgcg cgcgtggacg tcttcgagcc cgacgatcaa
      961 cctcgcggca tggtcaatgc tgccgacgtt cggatcctcg ctgccaggag cagctctggt
     1021 cgccactcgc tcgcgcacag cgacgcggaa caactcgatg gtttcggccg tcacggcaac
     1081 ccggttcccg gcaaggaccc gatcggccaa ttcaaggtct gcgcggggta gtgcgtactc
     1141 catagctgcg cgaactgcgc ccctcacggg gtcgagggtg cgctcgagtt ctttgcgcga
     1201 tgacagcacg acctgaccgg caacggtcat cacggcggcg gcaacagagg tgccgtcgcg
     1261 atccaccggc gttccgtcgg gcggacagca gtcggcgtcc gtgcaatgca acgaccacca
     1321 caagccgtcg aacacgcaca gcacgtcaac caccgcgatg cgggcctggt cgagttgtct
     1381 cgtcagggcg gcggcgatcc gacgcacgga tggatgcgat cgcccacccg acggcgggta
     1441 caacacgacg atcgcgaagt ctgcaccgtc gcgctttagg taaccgacga actcgcgcgc
     1501 gcaggcgttc gcagatcgcg ccgccggcaa gtcgacgcgg gccgtaagtc cgagccgttt
     1561 tcgctcgccc cgcagcgaca acacgacgag ggagttctcc ggctggaacc ccacgagata
     1621 gggcaccacc tcgacgatgt cggcaggtgt gctgaggcga aaatcgggct cgtcagtgtc
     1681 ggcaggatct gcgctaggtg cagtcatgcc gtgcagcgtg gccgctcaac agtgctcgca
     1741 acgagccgcg acgctcgttg tggacacggc cgcgatatgc acaggcccaa accccctagc
     1801 caggtggcta gtcgaccgcc cgttcgagct gcgccttcgt catcgccgac cggcccttca
     1861 cattcttgcg gcgagcctcg gcgtacaact gctccttcgt tctgccacca ggtccgcggt
     1921 gagagcgcag accgccgcgc cgtcccgacg aaatgtcgtg tatcgacgac acgctcgccg
     1981 tcggggactc gcctgcgcgc gcccgctcct tgttgaccgt gcgggcggcg atttcttcag
     2041 cgaggttctc caaacgcccc cgggcctcaa ggccatcccg gatatgttcg tattgccgct
     2101 cgcgcttggc actccacgcc cggggcatca ggcactgctc cgagtgaacg cgtggatcag
     2161 gccgaggatc agcgcaagga tcagtcctaa ccaaagcacg tgtgcggcga agcccaaccc
     2221 ggcgaacaac acgaataaga cgagagcaac gatgagcaac atgatgacct ctcgatgcag
     2281 tttggctgat ggtcacgcgc catcgttccc ggcaccgcga tcgtcaaaca cacgaccgaa
     2341 cgcaccctca acgaggctta gctacacggc cgaccgggta ggtcgatctt cgatatgacc
     2401 gccgcctgga ccttcgccgg agagagcccg tcgcccgggg tagggggcgg gc
//
LOCUS       DASWVF010000126         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_462238, whole genome shotgun sequence.
ACCESSION   DASWVF010000126 DASWVF010000000
VERSION     DASWVF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462238"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4016
                     /locus_tag="VGD55_02240"
     CDS             <1..4016
                     /locus_tag="VGD55_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0869192.1"
                     /translation="QLGFDASTFNVNGFLANSSTSADIRMTSASAGGETYYPGVITFA
                     TDLYSPDISATKSVALAAKGAGNTQAGVVEPGDTLQYTINATNTGQDTSVGSVLTDSI
                     PAGTTYVAGSLTSGGTALTDATGDDVGSFNAGTKQLTVDVGTGATSTVGGNVAVGASI
                     ATVTFEVVVNAAIADGAPIPNSAALAYKGNQTGIAVTGASNAVTSAAVRHHSALTITK
                     TANLTRVQKGAATPVTYTLTATNAGPYADPGVTVTDTLPTGAVVISATPSAGSCTTPA
                     GQVVCNIGALANGGTATVAVVVTLDGTSDPAADTATIAGSNIDSFPGDNTSSVSTVVN
                     TPPVAHPDSATTSGGTATIAVLANDTDADADPLTASAGSTAPTKGNIVVNANNTITYT
                     ANPGAVGTDTFSYIANDGRGGTSTATVTITIPNQLPVAVADSASTTPGQAVTVNVLSN
                     DTDPNIPASGQVLSVSAVTQPAGGTGTVTTDGSTVTFTPSGTFGKGSTSFTYTVSDGA
                     GGTAIGTVTVTIPNIAPAAADDTATTPYLTAVTVNVLANDTDANSDPLTVTAVSGEAH
                     GTAVITGSGVNTKVLYTPAVGWSGPDTMTYTISDGTTTSTAQLTVTTADGVPTANSFS
                     TSVPGGVQTTIDVLAHASDPNGDTLTVTGAGPAAHGTITVNGSGGVVYTPDPTYAGPD
                     SFSYTVDDGHGGTATGTVTLTVSNQPPVANDDSATLPFNTALDIAVLANDTDPNNDPL
                     TVSGVSTPAHGTATVLPSGAVHYVPAAGYIGPDSFSYTANDGHGGTSSATVNIAVIDQ
                     PPVANGDSAIATGLAGSPVTVDVLANDTDPDGDPLSIQSVSVPAHGAAAIVAGKVVYT
                     PSATYVGPDSFTYTIDDGHGGTSSATVTVDVQNRNPVANPDTLLSLSGIAANVDVVAN
                     DTDPDGETLALLSVDPTSTNGGTITIVGGVAQYTSTAAFVGTDTFNYVVADPRGGTAV
                     GSVTVTVGSSAMVANGRTASTSTDTPVLTDLLSGVTDPGGHPITVDSAGPAVHGTVTL
                     DASGNATYTPAVGFAGTDTYSYSVSDAFGGSATASVTVTVANANPVAVNDTASVVTGS
                     SLTVDVIANDTDPNIPGTSQVLDLSSASLVSGTATVSVAPGGILVVPGAGFTGDVVVQ
                     YTVSDGAGGTATGQLTVHVTAVVIPPVIPPVTPPLTPPLTPPLTPPGTPPLKPPAKPG
                     SPPKSVESTVLVVTITPSKTSVSIDPAHTGTGSGAVTIMSITNPTVGATVTVVNGLIV
                     VTRTSNFVGSTVFSYDATRADGTDVHVTVTAHVLGESLTGPSLPFTGANVDLVGLIAL
                     VLIGTGIVTIRIARERP"
     gene            complement(4037..4693)
                     /locus_tag="VGD55_02245"
     CDS             complement(4037..4693)
                     /locus_tag="VGD55_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0869193.1"
                     /translation="MSDDVKAAAKRRYSAPQRSAQAAATRRAVLASARELFVQRGYGA
                     TTVTDIAAGANVSVDTVYAAVGRKPVVLRELVETALSGADHAVVAQQRDYVVAIRAAP
                     TAREKLTIYATAVVKIQQRLAPVFIALRDAAVDDPACAALWAEISERRARNMREFAAD
                     LRETGELRDDLSDDEVADIVWSTNAAEYWVLLVHQRTWTPERFGEWLADTWIRSLLKQ
                     "
     gene            4773..>5071
                     /locus_tag="VGD55_02250"
     CDS             4773..>5071
                     /locus_tag="VGD55_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0869194.1"
                     /translation="MTEQLVRGGPVFRPANSGPATWAMGSLFERLVSGEESGDQLGAS
                     IVTQPPGIATPLHTHTHEAEAFYLLDGTMTYRAGEEIFHLGVGDFIYLPLGVPHA"
BASE COUNT          932 a         1820 c         1537 g          782 t
ORIGIN      
        1 accagctcgg cttcgacgca tcgacattca atgtgaacgg gttcctggcc aacagctcta
       61 ctagtgccga catccgtatg acgtctgcgt cggccggcgg cgagacctat tacccgggcg
      121 tcatcacgtt cgccaccgac ctgtactccc ccgacatctc ggcaacgaaa tctgttgcct
      181 tggcagcgaa aggtgcgggc aacacccaag ccggggtggt cgagcctggc gacacgttgc
      241 agtacacgat caacgccacg aacaccggcc aggacacgtc cgtgggctcc gtcctgactg
      301 actcgattcc ggccggaacg acgtacgtcg cagggtcact cacgagcggc gggacagcac
      361 tcaccgatgc gaccggagac gacgtcggta gcttcaacgc cggcaccaag cagctcaccg
      421 tggacgtcgg taccggcgcc acgtccacgg tcggcggcaa cgtcgccgtt ggtgcatcga
      481 tcgcgacggt caccttcgaa gtcgtcgtca acgcagccat tgccgatggc gcgccgatcc
      541 cgaacagtgc ggcacttgcg tacaagggca accagaccgg tatcgcggtc accggcgcga
      601 gcaacgctgt cacctcggct gcggtccgcc accacagtgc tctcaccatc acgaagaccg
      661 ccaacctcac ccgggtgcaa aagggtgccg cgactccggt cacctacacc ttgacggcca
      721 ccaacgctgg gccctacgcc gaccccggcg tgacggtcac cgacaccttg cccaccggcg
      781 ccgtcgtgat ttcagcgacg ccgagcgcag gtagttgcac cacaccggcc ggccaggtcg
      841 tgtgcaacat cggcgcgctc gcgaacggcg gcacggccac ggtcgcggtt gtcgtcaccc
      901 ttgatggcac cagcgatccg gccgccgaca cggccaccat cgcgggctcg aatatcgaca
      961 gcttcccagg tgacaacacc tcgtcggttt cgaccgtcgt gaacacacca ccggtcgcgc
     1021 accccgactc ggcaaccacg tcaggcggca ccgccaccat tgccgttctt gccaacgaca
     1081 ccgacgcgga cgccgacccg ctcaccgcgt ccgcgggctc gaccgcgccc accaagggca
     1141 acatcgtcgt caacgcgaac aacacgatca cctacacggc caaccccggc gccgtcggta
     1201 ccgacacgtt cagctacatc gcgaacgacg gccgaggcgg cacatcgacg gcgaccgtga
     1261 cgatcaccat cccgaaccaa ctgcctgtcg cggtcgccga ctcagcaagc acgacaccgg
     1321 gtcaagccgt gacggtaaac gtgctgagca atgacacgga ccccaacatc ccggcgtccg
     1381 gtcaagtttt gtccgtcagc gctgtgacac aacccgcagg cggcactgga acggtcacca
     1441 ccgatggttc gacggtgacc ttcaccccgt ctggcacctt cgggaagggc agcacctcgt
     1501 tcacgtacac ggtgagcgac ggcgccggcg gaaccgcgat cggcaccgtg acggtgacga
     1561 tcccgaacat cgcaccggcg gccgcagacg ataccgcgac gacgccttac ctcacagccg
     1621 tgacggtcaa cgtgctggcc aacgacaccg acgccaacag cgatccgctc accgtgaccg
     1681 ccgtatcggg tgaggcacac ggcaccgcgg tcatcaccgg atccggcgtg aacacaaagg
     1741 tgctgtacac accggccgtc ggctggagcg gccccgacac gatgacgtac acgatcagcg
     1801 atggcacgac cacctcgacg gcgcagctca ccgtgacaac ggccgatggc gtcccgaccg
     1861 caaacagttt cagcaccagc gtccccggtg gcgtgcagac cacgatcgac gttctcgcgc
     1921 acgcgagtga ccccaacggc gacacgttga ctgtcaccgg agccggacct gctgcgcacg
     1981 ggaccatcac ggtcaatggc agcggcggag tcgtgtacac ccccgacccg acgtacgccg
     2041 gacccgattc cttcagctac acggtcgatg acgggcacgg cggaaccgcg acaggcaccg
     2101 tcacgctgac cgtcagcaac cagccaccgg tggctaacga cgacagcgcg acgctgccgt
     2161 tcaacaccgc gctcgacatt gcggtgctgg cgaacgacac cgaccccaac aatgatccac
     2221 tgacagtttc gggcgttagt acaccggcgc atggcaccgc gacggttctg ccgagcggcg
     2281 cggtgcacta cgtcccggcg gccggttaca tcggccccga ctcgttctcg tacaccgcca
     2341 atgacgggca cggcgggacg tcgagcgcta ccgtcaacat cgcggtgatc gaccagccac
     2401 cggtagcgaa cggcgattcg gcgatcgcga ccggacttgc gggctcaccg gtcaccgtcg
     2461 acgtccttgc caatgacacc gaccccgacg gcgacccgct ctcgatccaa tcggtctcgg
     2521 tgccggcgca cggagccgcg gccatcgtcg ccggcaaggt cgtctacacg ccctcggcca
     2581 cgtacgtcgg tcccgactcc ttcacctaca ccattgacga cgggcacggc gggacgtcga
     2641 gtgcaaccgt cactgtcgac gtgcagaacc gtaacccggt cgcgaacccc gacaccctgc
     2701 tttcattgag cggaatcgcg gcgaacgtcg acgtcgtcgc gaacgacacc gaccccgatg
     2761 gcgaaacgct tgcgctactt tcggtcgacc cgacaagcac gaacggcggc acgatcacga
     2821 tcgtcggcgg cgtcgcgcag tacacctcga ctgcggcctt cgttggcact gacacgttca
     2881 actacgtcgt cgccgacccg cgcggcggca cggcggtcgg cagcgtcacg gtcaccgtgg
     2941 gcagctccgc gatggtcgca aacggtcgca cggcgtcgac gtcgaccgac acgcccgttc
     3001 tcaccgatct gctctccggc gtcaccgacc cagggggtca cccgatcacc gtcgactccg
     3061 cagggccggc cgtacacgga accgtgacac tcgacgcttc cggcaatgcg acctacacgc
     3121 ccgcagtggg tttcgccggc acggatacct actcctactc ggtttctgac gcattcggcg
     3181 gttcggcaac ggcatcggtg acggtgacgg ttgccaacgc caacccggtc gccgtgaacg
     3241 acactgcgtc agttgtcacc ggatcgtcgt tgaccgtcga cgtgatcgcg aacgataccg
     3301 acccgaacat ccctggcacg tcgcaggtgc ttgacttgtc atcagcaagc ctcgtcagcg
     3361 ggacggcgac ggtgagcgtt gcgccgggcg gcattctcgt ggttccaggt gctggattca
     3421 ccggtgacgt cgtcgtgcag tacaccgtca gcgatggcgc cggcggcact gcgacgggac
     3481 aactgacggt tcatgtcacg gcggttgtca tccctcccgt gatcccacct gtcacgccgc
     3541 cgctgacgcc tccgttgacg ccgccgctga cgcctccagg tacgcccccg ctcaagccgc
     3601 cggcgaagcc cgggtcgcca ccgaagtcgg tcgagtcgac tgtccttgtc gtgacgatca
     3661 cgccgtcgaa gacctcggtc agcatcgacc cggcacacac cggtaccggg agcggtgctg
     3721 tgacgatcat gtcgatcacc aacccgacgg tcggagcgac cgtcaccgtc gtcaacggtt
     3781 tgatcgtcgt gacgcgcacg tcgaacttcg tcggcagcac ggtcttcagc tacgacgcga
     3841 cgcgcgcgga cggcaccgac gtgcacgtca ccgtcacagc gcacgttctg ggcgaatcgt
     3901 tgaccggacc atcgctgccc ttcaccggcg cgaacgtcga cctcgtcggg ttgatcgcac
     3961 tcgtgctcat cggcaccggc atcgtgacca tcaggatcgc gcgcgagcgt ccgtagcggt
     4021 tccgtggttt agcgattcac tgctttagca gcgacctgat ccaggtgtca gcgagccact
     4081 cgccgaaacg ctccggcgtc cacgtgcgtt ggtggactag cagcacccag tactcggccg
     4141 cattcgtact ccacacgatg tcggccacct cgtcatcgga caggtcgtcg cgcaactcgc
     4201 cggtctcgcg aaggtcggct gcaaactcgc gcatgttgcg ggctcggcgc tcgctgatct
     4261 cggcccacag cgccgcacac gcaggatcgt cgacggctgc gtcgcgtagg gcgatgaaca
     4321 ccggcgcaag tcgctgctga atcttcacca cagcggtggc atagatcgtc aacttctcgc
     4381 gagcagtggg cgccgcgcgg atcgcgacga catagtcgcg ctgctgggcg acgaccgcgt
     4441 gatcggcacc cgacagcgct gtctcgacaa gctcgcgcag cacgacgggc tttcgaccga
     4501 ccgcggcgta cacggtgtcg accgagacgt tcgcgccggc tgcgatgtcg gtaaccgttg
     4561 tcgccccgta gccgcgctgc acaaagagct cgcgggcgga agcgagcacc gcgcgccgcg
     4621 tggcagccgc ttgggccgac cgctgcgggg cgctgtagcg gcgcttcgcc gcggccttga
     4681 cgtcgtcgct catcatggct agcttagcaa tattcgtccg aaactgcttc gggtgaaatg
     4741 catccggagg cttccatgac ggagcaattg gcatgacgga gcaattggtg agagggggtc
     4801 ccgtcttccg gccggccaac tcgggccccg ccacgtgggc gatgggctcg ctgttcgagc
     4861 gcctggtcag cggcgaggag tcgggcgacc agctcggggc gtcgatcgtc acgcaaccgc
     4921 ccggcatcgc cacgcccctg cacacccaca cgcacgaggc ggaagcgttc tacctactcg
     4981 acggcaccat gacctaccgg gcgggcgaag agatctttca cctcggcgtc ggcgacttca
     5041 tctacctgcc gctcggggtt ccgcatgcgt t
//
LOCUS       DASWVF010000127         4448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_156385, whole genome shotgun sequence.
ACCESSION   DASWVF010000127 DASWVF010000000
VERSION     DASWVF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156385"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGD55_02255"
     CDS             complement(<1..266)
                     /locus_tag="VGD55_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY0869195.1"
                     /translation="MDQRWLDLVDTRANWPSRNPVERLPDADVLARWLRDHGLSAELA
                     PTAADVAMVRQVRSSLRELAVGRIRGRTPHPQALDTVNAALARAA"
     gene            394..2025
                     /locus_tag="VGD55_02260"
     CDS             394..2025
                     /locus_tag="VGD55_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0869196.1"
                     /translation="MSTLAARGIRVSFGALEILHGVDVTISTGDRVGLVGPNGAGKST
                     LLRVLAGELTADAGTVTSTGTVGLLPQEPDRRPGETLLAYLGRRTGVATAEAAMETAA
                     DGLATSVSGAAETYDEALNRWLDLGGGDFDSRAREVVTDLGLDLASLEIETAGLSGGQ
                     MARASLAAVLLSRYDILLLDEPTNDLDLDGLARLEDFVLGRRGGLAVVSHDREFLSRT
                     ITHVVELDPDNGLTRQFSGGWEVFLEEQEIARRHLREQFDGYADKRGDLRDRMQAARE
                     QSVRGALRAKRKAPDNDRSARGARIEAATSGAAKVRSLETRLARLDAEAVTEPRKVWK
                     LHIELPSAPRSGEIVATLHDAVVVRGDFTLGPVSLDVRWADRIAVLGPNGGGKTTLLQ
                     ALLGHLALTSGTATLGPGVVVGELDQVRREFAGSQTVVDVLARATRLVPEEARTLLAK
                     FGLKAAHVERSSGSLSPGERTRAGLALVMARQTNCLVLDEPTNHLDMPAIEQLEQAVA
                     EYTGTLLLVSHDRRLLEAVPTKRRLHVEHGRVRED"
     gene            2102..2488
                     /locus_tag="VGD55_02265"
     CDS             2102..2488
                     /locus_tag="VGD55_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869197.1"
                     /translation="MPRHVLALVLIAALILLGRWQWDVSESSRGGLQNLLYAFQWWAM
                     AAMVVYGWWRLLRDDAYGRPPARSAPARVALSTTEATWDFNEFATPDAFASLTAQTTD
                     DRDPDPELAEYNDYLTKLNARSERAR"
     gene            2485..2835
                     /locus_tag="VGD55_02270"
     CDS             2485..2835
                     /locus_tag="VGD55_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEY0869198.1"
                     /translation="MNLKSPLDRYRLLAYIVGTALVILVFIGIPLQVWGHNDTVEKIV
                     GTVHGFLYIVYVVLSLELAFRYRFSLVRTVGSLLAGLVPIMTFVAERKNTAYVRARLA
                     DTERPPVDVPTSAN"
     gene            2849..3826
                     /locus_tag="VGD55_02275"
     CDS             2849..3826
                     /locus_tag="VGD55_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY0869199.1"
                     /translation="MARTRLYRNGVLEKENFPVADISECVNDPGVVVWLDICVLDDET
                     GLDDVEEEFGLHALAVEDAAHERQRPKLDHYGTHLFISAYAIALNPQTMDLESSELAM
                     FVMPNALITVRKDRKFDIDEVVKRWDATPELVKEGAGFLLYGVLDYIVDGHFQAVQDL
                     DERIEGLQDDLFDDSRDRVREVQRKSFDLRRDLILLRRFVLPMREVVNGLMRRDVKVV
                     NDTLMPYFQDVYDHVLRATEWTESLRDLVSTILETNLTIQGNRMNLIMKQVTSWAAII
                     AVPTAVTGFYGQNLPYPGFQNNDGLLTSSLIIALGSSALYFVFRRKGWL"
     gene            complement(3831..4040)
                     /locus_tag="VGD55_02280"
     CDS             complement(3831..4040)
                     /locus_tag="VGD55_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869200.1"
                     /translation="MAIVRCSQGGLFETTWIPWASFKAVRLGRRRLQRCPVHRKWEIV
                     ERVDPATLSEAQLREAARYPAGWLP"
     gene            4109..>4448
                     /locus_tag="VGD55_02285"
     CDS             4109..>4448
                     /locus_tag="VGD55_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0869201.1"
                     /translation="MKVVLAGGSGALGRRLAAAASARGDEVVVLTRTPRPGIAYRQVR
                     WDGQSVGAWADELADAVLINLAGELVDRRPSASNIALLTRSRVQPTAALVAASRSATP
                     VLWVQLSTLAI"
BASE COUNT          753 a         1458 c         1465 g          772 t
ORIGIN      
        1 gccgcgcggg cgagcgcggc gttcaccgtg tcgagcgcct gcgggtgcgg cgtccgcccg
       61 cgaatgcgtc caaccgccaa ctcgcgcagc gacgacctca cttgccggac catcgcaaca
      121 tcggccgccg tcggcgcaag ctcggccgaa agcccatggt cgcggagcca tcgcgcgaga
      181 acgtcggcat cgggaagacg ctcgaccgga ttgcgcgacg gccagttcgc gcgagtgtcg
      241 acgagatcga gccagcgctg atccattcga tgaccgtaac gtcttaaccc gttacggagg
      301 cttcgcgcca cgctgatcga tagcggtaac gcctgcaagc gttacgtctg ttcgatgtgc
      361 ggcatgggtg gcatgactgg caggatcacg gccgtgtcga ctctcgccgc ccgtggcatc
      421 cgcgtcagtt tcggcgccct cgagatcctg cacggcgtcg acgtcacgat cagcacgggt
      481 gaccgagtcg gtcttgtcgg acccaacggc gcggggaagt cgacgctcct gcgagtgctt
      541 gccggcgaac ttaccgccga cgctggcaca gtcacgtcaa ccggcacggt cggcctgcta
      601 ccccaggaac ccgaccgacg acctggggag acgctgctcg cctacctcgg ccgccgcacc
      661 ggcgttgcga cggctgaggc cgcgatggag acggctgccg atggcctggc cacgagcgtc
      721 tccggtgcag ccgaaaccta cgacgaggcg ctcaaccgct ggctcgatct gggcggtggc
      781 gacttcgaca gccgtgcgcg cgaggttgtg accgacctcg gacttgacct cgcgtcactc
      841 gagatcgaaa ccgcagggtt gtcaggcggg cagatggcga gagcgtcgct ggctgctgtc
      901 ttgttgagcc gctacgacat cttgctgctc gacgaaccga ccaacgacct cgatcttgac
      961 ggcctcgcca ggctcgaaga cttcgttctc gggcgtcgcg gcgggctcgc cgtggtcagc
     1021 cacgatcgcg agttcctgtc ccggacgatc acccacgtcg tcgagctaga tcccgacaac
     1081 gggttgacgc ggcagttctc tggcggctgg gaggtgttcc tcgaggagca ggagatcgcc
     1141 cgccgccatc tgcgcgagca gttcgacggc tacgccgaca agaggggtga cctgcgcgat
     1201 cgcatgcaag ccgcgcgaga gcagtcggtg cggggtgcgc tgcgcgccaa gcgcaaggcg
     1261 ccagacaacg accgttcggc gcggggtgcg cgcatcgagg cggcgacgtc gggcgccgca
     1321 aaggtccgct cgctggagac tcgactcgcc aggttggacg ccgaggccgt gacggagcct
     1381 cgcaaggtgt ggaagctgca catcgaactg ccaagcgcgc ccaggtcggg tgagattgtc
     1441 gcgacgctgc acgacgcggt ggttgtgcga ggcgatttca cgctcgggcc ggtcagcctc
     1501 gacgtccggt gggccgatcg catcgccgtg ctcggaccca atggcggtgg taagacgacc
     1561 ctgttgcaag cgctgctcgg ccacctcgcg ttgacgtcag gcaccgcaac ccttgggccg
     1621 ggagtcgtgg tgggcgagct cgaccaggtg cggcgcgagt tcgccggcag ccagaccgtg
     1681 gtcgacgtcc ttgcgcgggc gacccggctc gtgcccgagg aggcccgcac tttgctggca
     1741 aaattcggcc tcaaggccgc ccacgtcgaa cggtcgtcgg gctcgctgtc tccgggcgaa
     1801 cgaacccgcg ccgggcttgc cctggtgatg gcacggcaga ccaactgctt ggtgctcgac
     1861 gaaccgacca accacctcga catgcctgcg atcgagcaac tcgagcaggc agtcgctgag
     1921 tacaccggca ccctgttact cgtcagccac gaccgccggc tgcttgaggc ggtaccgacg
     1981 aagcgccggc tgcacgtcga gcacgggcgg gtgcgcgagg actaaggcgg tacccacgac
     2041 caaccggcgc gtgacgggta ccgtggagtc cgtgctcagg accgcgtttc ggttgcgatg
     2101 gttgccgcgc catgtgctgg ccctcgtgct gatcgccgcc ctgatcctgc tggggcgctg
     2161 gcagtgggac gtctccgaat cgtcacgcgg cggcttgcag aatctcctct acgccttcca
     2221 gtggtgggcc atggcggcga tggtcgtgta cggctggtgg cggctcttgc gcgacgacgc
     2281 gtacggccgc ccgccagcgc gcagcgcgcc tgctcgcgtg gcgctttcga caactgaagc
     2341 cacgtgggac ttcaacgagt tcgccacccc cgatgccttc gccagcctca cggcccagac
     2401 caccgacgac cgggaccctg atcccgagct ggccgagtac aacgactacc tcaccaagct
     2461 gaacgcccgc tccgagcgtg cgcgttgaac ttaaagtctc cgctcgatcg ctaccgactg
     2521 ctcgcgtaca tcgtgggcac cgcgctggtg attttggtgt tcatcggaat cccgctgcag
     2581 gtgtgggggc acaacgacac ggtggaaaag atcgtgggga ccgtccacgg ctttctgtac
     2641 atcgtgtacg tcgtgctaag cctcgaactc gcgttccgtt accgcttctc actcgtgaga
     2701 acggtcggct ctttgctcgc gggcttggtg ccgatcatga ccttcgtggc tgagcggaag
     2761 aacacggctt acgtgcgagc acggttggct gacactgaac ggccacctgt cgacgtcccg
     2821 acttctgcga actgaccgga gcaacgagat ggcgcgcaca cggctgtatc gcaatggggt
     2881 gctcgagaaa gagaacttcc cggtcgccga tatttccgaa tgtgtcaacg acccaggcgt
     2941 ggtcgtgtgg cttgacatct gcgtcctcga cgatgaaacg ggcctcgatg acgtcgagga
     3001 ggagttcggg ctgcacgctc tagccgttga ggacgcggct cacgagcggc agcgcccgaa
     3061 gctcgaccac tacggcaccc acctgttcat cagcgcctac gcgatcgctc tcaatccaca
     3121 gacgatggac ctcgagagca gcgagctcgc gatgttcgtc atgccgaatg cgctgatcac
     3181 ggttcggaag gatcgcaagt tcgacattga cgaagtcgtc aagcgctggg atgcgacccc
     3241 cgaactggtc aaggaaggcg ccggattctt gctgtacggc gttctcgact acatcgtcga
     3301 cgggcatttc caagcagtgc aggatcttga cgaacgcatc gagggactac aggacgacct
     3361 gttcgacgac agccgcgatc gggtgcgcga ggtgcagcgc aaatcgttcg acttgcgtcg
     3421 cgatctgatc ctgctgcgcc ggttcgtctt gccgatgcga gaggtcgtca acgggctgat
     3481 gcgccgcgac gtcaaggtcg tcaacgacac cttgatgccg tacttccagg acgtttatga
     3541 ccacgtgctg cgtgcaactg agtggaccga gtctctgcgt gatctggtgt cgaccattct
     3601 cgagacgaac ctgacgatcc agggcaaccg gatgaacctc atcatgaagc aggtcaccag
     3661 ttgggccgcg atcatcgccg tgccaaccgc ggtgacaggc ttctacggcc agaacctgcc
     3721 ctaccccggc ttccagaaca acgacggctt actcacgtcg tcgctcatca tcgccctcgg
     3781 ctccagcgcg ctgtacttcg tctttcgccg caagggctgg ctctagcgtt ctagggaagc
     3841 caccctgcgg ggtatcgggc tgcctcgcgc agctgcgctt ccgacagggt ggctggatcc
     3901 actcgctcga cgatctccca cttgcgatgg actgggcagc gctggagacg acggcgtccc
     3961 aggcgcactg ccttaaacga cgcccacgga atccacgtgg tctcgaagag gccgccctgg
     4021 ctgcaccgca cgatcgccac gggtcgaagg tactcccgca acttgagcgc tacggaatca
     4081 cggacgccgg cgatgcgacg atgagccggt gaaggttgtg ctggctggcg ggtctggcgc
     4141 tctgggtcgc cggttggccg ccgccgcgtc cgcgcgcggc gacgaggtcg tcgtcctgac
     4201 ccgcacaccc cgaccaggca ttgcgtatcg ccaggttcga tgggacggcc aatccgtcgg
     4261 agcttgggct gacgaactcg ctgacgcggt gctgatcaac ctcgccggcg agctggtcga
     4321 ccgccgcccc tcggcgtcca atatcgcgct gttgacccgc agtcgtgtgc aaccgaccgc
     4381 cgcgctggtg gcggcgagca ggtctgcgac acccgtgctg tgggtgcagt tgagcacgct
     4441 cgccatct
//
LOCUS       DASWVF010000128         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_334742, whole genome shotgun sequence.
ACCESSION   DASWVF010000128 DASWVF010000000
VERSION     DASWVF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334742"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1906
                     /gene="aceE"
                     /locus_tag="VGD55_02290"
     CDS             <1..>1906
                     /gene="aceE"
                     /locus_tag="VGD55_02290"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY0869202.1"
                     /translation="FFQGHASPGIYARAFLEGRLTEAQLDGFRQEVSSHGLPSYPHPR
                     LMPDFWEFPTVSMGLGPLDSIYQARFNRYLHARGIADTSDSHVWAFLGDGEMDEPESL
                     GAIGVAAREELDNLTFVINCNLQRLDGPVRGNGKIIQELEAHFRGAGWNVIKVIWGGD
                     WDRLFAKDVDGALVNKLNSTPDGQLQTYSVESAQYIRDHFFGDDPRLRKMIEGMSDAD
                     LRRLSRGGHDYRKVYAAFAAAREHVGQPTVILAHTIKGWTLNSLQGRNATHQMKKLTK
                     TDLKEFRDRLHLPISDAALEADLPPYYHPGMESEEIHYMLERRRALGGSLPERINRAR
                     LLKLPGDAVYAELRQGSGKQKVATTMGFVRLLKDLMKEDDIGARIVPIIPDEARTFGM
                     DSLFPTAKIYSPHGQQYDAVDRDLVLSYKESETGQLLHEGITEAGAMGSFIAAGSAYA
                     THGQYMLPVYIFYSMFGFQRTGDQIWAAADQMCRGFLIGATAGRTTLTGEGLQHNDGH
                     SPLLASTNPAVVYYDPGFAYEIGHIMHSGLARMYSATAAHPAGEDVFFYITVYNEPIL
                     QPAQPENLDVEGLLAGLYHVASPSASVLDAPRAQLLASGDALPWALEAQRMLAEDWGV
                     GADVWSATSWT"
BASE COUNT          367 a          636 c          568 g          335 t
ORIGIN      
        1 cttcttccag ggccacgcct cccccggcat ctacgcgcgc gcatttctcg aaggccgcct
       61 gaccgaggcg cagctcgacg gattccggca ggaggtgagc agtcacggcc tgcccagcta
      121 cccacatccg cgattgatgc cggacttctg ggagttcccg acggtctcga tgggcctcgg
      181 tccgctcgac tcgatctacc aggcgcggtt caaccgctat ctgcacgcac gcggcatcgc
      241 cgatacgtcc gactcgcacg tgtgggcgtt tcttggcgac ggcgagatgg acgagcccga
      301 atccctcggc gcgatcgggg tcgccgctcg cgaagaactc gacaacctga ccttcgtcat
      361 caactgcaat cttcagcgac tcgacgggcc ggtgcgcggc aacggcaaga tcatccaaga
      421 actcgaggcc cacttccgcg gcgccggatg gaacgtcatc aaggtgatct ggggcggcga
      481 ctgggatcgc ctcttcgcga aagacgtcga cggcgcgctc gtcaacaaac tcaactcgac
      541 gcccgacgga cagttgcaga cctattccgt cgaatccgcg cagtacatcc gcgaccattt
      601 cttcggcgac gatccgcggc ttcgaaaaat gatcgaaggc atgtcggacg ccgatttgcg
      661 ccgtctctcg cgcggcggcc acgactatcg caaggtgtat gcggcgttcg ccgcagctcg
      721 cgaacatgtc ggtcagccga cggtgatttt ggcgcacacc atcaagggct ggactctcaa
      781 cagcctgcaa gggcgcaacg ccacgcacca gatgaagaag ctcacgaaga ccgatctgaa
      841 ggagttccgc gaccggctgc acctgccgat cagcgatgcg gcgctcgagg ccgacctacc
      901 gccgtactac cacccgggca tggagtcaga agagatccac tacatgctcg aacgccgacg
      961 ggcgttgggc ggatcgttgc ccgagcgcat caatcgcgct cggttgctca agctgcctgg
     1021 cgatgctgtg tacgccgagc tgcgtcaggg atctggcaag caaaaggtcg cgaccacgat
     1081 gggtttcgtg cggctgctca aagacctaat gaaggaagac gacatcggcg cgcggatagt
     1141 gccgatcatc cccgacgagg cacgcacctt cggtatggat tcactatttc cgacggcgaa
     1201 gatctactca ccgcacggcc agcaatacga tgccgtcgac cgcgatctcg tgctgtcgta
     1261 caaggaatcc gagaccggcc agctattgca cgagggcatc accgaagctg gcgcgatggg
     1321 ctcgttcatc gcagccggat ccgcgtacgc aacacacggc cagtacatgc tgccggtcta
     1381 catcttctat tcgatgttcg gcttccagcg caccggtgat cagatctggg cagcagccga
     1441 tcagatgtgc cgcggtttcc tgatcggtgc caccgcgggc cgcacaaccc ttacaggcga
     1501 gggattgcag cacaacgacg gtcattcgcc gttgctcgcc tcgacgaatc cagctgtcgt
     1561 ctactacgac cctgggttcg cctacgagat cggccacatc atgcattccg gactcgcccg
     1621 catgtacagc gcgaccgctg cgcacccggc cggtgaggac gtcttcttct acatcaccgt
     1681 gtacaacgag ccaatcctgc aacccgcgca gcccgagaat ctcgacgtcg agggattgct
     1741 cgccggcctt tatcacgtcg ccagcccctc ggcgtccgtg ctcgatgcac ctcgggctca
     1801 gctgctcgcg tcgggcgatg cactgccgtg ggcgctcgag gcccaacgca tgctcgccga
     1861 agactggggc gtcggagccg acgtctggtc ggcgacgagc tggacc
//
LOCUS       DASWVF010000129         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_131095, whole genome shotgun sequence.
ACCESSION   DASWVF010000129 DASWVF010000000
VERSION     DASWVF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131095"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGD55_02295"
     CDS             complement(<1..228)
                     /locus_tag="VGD55_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869203.1"
                     /translation="MSAGSSNSMAGLTGSYRVGVVGATGQVGTVIRQILAERGFPVSQ
                     MRYFASVRSAGTTLPWDGADVVVEDGLLADPS"
     gene            complement(225..1493)
                     /locus_tag="VGD55_02300"
     CDS             complement(225..1493)
                     /locus_tag="VGD55_02300"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0869204.1"
                     /translation="MGLVVQKYGGSSVADAERIKKVAQRIVATRKEGHEVCVVVSAMG
                     DFTDELIELAAEVSPLPPARELDMLLTAGERISMALLAMAIANLGMEARSFTGSQAGV
                     ITNSAHGKAQIIDVAPSRIREALDQGHIAIVAGFQGVSQDTKDVTTLGRGGSDTTAVA
                     LAVALGAEVCEIYTDVDGVFTADPRLVPTARRIERLSYEEMLEMAASGAKILHLRCVE
                     YARRYGLPIHVRSSFSTKTGTWIAEIPAEELMEAPIISGVAHERGEAKITVVGVPDKP
                     GEAAQIFTAVADAGVNIDMIVQNVSSVATGLTDITFTLPSTDLSAAVAALSRAQASIG
                     FESLQSDDQIGKVSLVGAGMRTHPGVTARFLSALADAGINVEMISTSQIRISVVVRED
                     ELPIAVAAVHSAFELDSPDDVATVYGGTGR"
     gene            1595..>2251
                     /locus_tag="VGD55_02305"
     CDS             1595..>2251
                     /locus_tag="VGD55_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869205.1"
                     /translation="MALTLAATGCTSHHTNPAPSSEPTSSGPGAAAALATIAQVARAA
                     AYTATYSAQSSENPPRSNIISVFRTSTQTRLDVTGATGHVLIQVDPTGTYTCKLPASG
                     SPSCLTLAGPGEAVPASVDPEGQALFTTTLDVLAQGNDITVATEAPRPAENGVPVASC
                     YAVIAAPAGGVSPGTYCFTATGILTRAQFRTSVLQLTALSGTPVDTDFTLPASPLPLS
                     A"
BASE COUNT          404 a          779 c          708 g          360 t
ORIGIN      
        1 ggacgggtca gctagcaaac cgtcttcgac gacgacgtcg gcgccgtccc agggcaacgt
       61 cgttccggcc gagcggaccg atgcgaaata gcgcatctgc gacaccggga acccgcgctc
      121 ggcgaggatc tgccgaatca cggtaccgac ctgcccggtt gcaccgacaa caccgacgcg
      181 gtacgagccg gtcaagccag ccatcgagtt cgaagagccc gcactcatcg cccggttcct
      241 ccgtagacag tcgcgacgtc gtccggcgaa tcgagttcga acgcgctgtg caccgctgcg
      301 accgcgatcg gcagctcgtc ctctcggacg acgacggaga tgcgaatctg cgacgtcgag
      361 atcatctcca cgttgatgcc tgcatctgcc agtgcggata agaaccgcgc cgtcacgccg
      421 gggtgcgtgc gcatcccggc gccgacaagt gacaccttgc cgatctggtc gtcggactga
      481 agcgactcga acccaatcga cgcctgtgct cgagaaagcg cagcgacggc agcagagaga
      541 tcggtgctag gcagcgtgaa ggtgatgtcg gtgagaccgg tcgccaccga cgatacgttc
      601 tgcacgatca tgtcgatgtt cacaccggca tcggcgacgg cggtgaagat ctgcgcggcc
      661 tcgcctggct tgtcgggcac tcccaccacc gtgatcttgg cttcgcctcg ctcgtgggcg
      721 acgccggaga tgatgggagc ttccatgagt tcctccgcgg ggatttcggc gatccaggtt
      781 ccggtcttcg tcgagaacga cgagcgcacg tggatcggca agccataacg ccgggcgtac
      841 tcgacgcagc gcaagtgcag aatcttcgca ccggatgcgg ccatctcgag catctcttcg
      901 tacgagagac gctcgatgcg ccgagccgtc ggaacgagac gcggatcggc ggtgaacacg
      961 ccgtcgacgt ccgtgtaaat ctcgcagacc tcagccccta gagccaccgc caacgcgacc
     1021 gcggtggtgt cggaaccgcc acgaccgagg gtcgtcacgt ccttggtgtc ttgggacacg
     1081 ccctggaatc cggcaacgat cgcgatgtgg ccctggtcga gtgcctcacg gatccggctc
     1141 ggcgcgacgt cgatgatctg cgctttcccg tgggccgagt tcgtgatcac gccggcctgg
     1201 ctcccggtga aagagcgggc ttccatgccg agattcgcga tcgccatggc cagcagggcc
     1261 atcgagatcc gctcgcccgc cgtcagcaac atgtcgagct cgcgcgccgg aggcaggggc
     1321 gagacttccg ccgcgagttc gatcaactca tcggtgaaat cgcccatggc tgagaccacg
     1381 acgcacacct cgtgaccctc tttgcgcgtc gcaacaatgc gttgcgcgac cttcttgatg
     1441 cgctcagcgt cggcgaccga tgagccaccg tacttttgga cgacgaggcc cacgggcgaa
     1501 cactccttga gggggaagac cgcaggcaag tctaccgggg cccgttagcc tgactcgtgt
     1561 gcatttcgta aggacggcgg ttggggccgc aggtgtggcg ctcaccctcg cggcaactgg
     1621 ctgcacgagc caccacacca acccggcgcc ttcgtccgag ccgacgtcct cggggccggg
     1681 tgcggctgcg gcgctcgcga cgatcgccca ggtcgcccgc gcggccgcct acacggcgac
     1741 ctacagcgct caaagctccg aaaatcctcc gcgaagcaac atcatcagcg tgttccgcac
     1801 cagcacccag acccggctcg atgtgaccgg ggccaccggc cacgtgctaa tccaggtcga
     1861 cccgaccggc acctacacct gcaagctgcc agcgtccgga tcgccgagct gcctgacgct
     1921 ggccggtcca ggggaagcag tccccgcaag cgtcgaccct gaggggcagg ccctgttcac
     1981 gaccactctc gacgtgctcg cgcaaggcaa cgacatcacc gtcgcgaccg aagctccacg
     2041 cccagcggag aacggagtcc cggtcgcttc ctgctacgcc gtgatcgccg cgccggccgg
     2101 tggcgtatcg cctggcacct actgctttac ggcaacgggg attctcacgc gcgcgcagtt
     2161 ccggacgagc gtcctgcagc tgacagcgct gtctggtaca ccggtcgata ccgacttcac
     2221 cctgccggcg agcccgctcc cgctcagcgc g
//
LOCUS       DASWVF010000130         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_207553, whole genome shotgun sequence.
ACCESSION   DASWVF010000130 DASWVF010000000
VERSION     DASWVF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207553"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..654)
                     /locus_tag="VGD55_02310"
     CDS             complement(109..654)
                     /locus_tag="VGD55_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0869206.1"
                     /translation="MLRTRLAAVLIPLVITSTPVAFGAAPAPLGDSLSDLHAVSQWAV
                     KLSEMADKRAKSELVKSYAQTVATTNPTLDAKLVSVAKSQGIDITPPEPQTEERRSLN
                     ERMKAETTLLGSLEGDAFDKEYMTLVTNTQQSVLNLLKASKSSATDPEVKKLISELTT
                     TIQTRLKTAQDVMSKVYGHQI"
     gene            complement(791..1429)
                     /gene="udk"
                     /locus_tag="VGD55_02315"
     CDS             complement(791..1429)
                     /gene="udk"
                     /locus_tag="VGD55_02315"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEY0869207.1"
                     /translation="MSVPVSRSFTLIGIAGGTASGKTTVARRIIEALPAGQAALIQQD
                     AYYRDRTALTPAQRAGLNFDEPDALENDLLLQHLRALKAGQPVACPEYDFTTHTRKSS
                     VRRIDPCPVIVVEGILLLAVPTLRDVFDLRLYVDTDDDVRLIRRIKRDMLERGRDIRS
                     IEAQYMGTVRAMHRLHVEPSKQHADLIIPEGGENAPALDVIGGRLLYGLLPR"
BASE COUNT          272 a          549 c          595 g          365 t
ORIGIN      
        1 ccgtcgatgt cgccggacga ctcgagagct tccatccgac gcccacgact accgcgatga
       61 cggcaagcgc tgctgcaatg tgccttttca tgatgatctc cgttcgcctc agatctggtg
      121 gccgtagacc ttcgacatga cgtcttgggc cgtcttgaga cgcgtctgga tggtggtcgt
      181 caattcgctg atgagtttct tgacctccgg atcggtcgcc gagctcttgc tggccttcag
      241 caagttgagg acgctctgct gggtgttggt cacgagggtc atgtactcct tgtcgaaggc
      301 atccccttcg agagacccga gcagcgtcgt ttcggccttc atgcgctcgt tcaggctcct
      361 gcgctcctcg gtttgcggct ccggaggcgt gatgtcgatg ccctggctct tggcgacgga
      421 aacgagcttc gcgtccaaag tcgggttggt cgtcgcgacc gtctgggcat agctcttcac
      481 gagctccgac ttcgccctct tgtcggccat ctcactgagc ttgacagccc actggctgac
      541 cgcgtgcagg tccgagagac tgtcacccag aggggccggc gcagcgccaa acgccactgg
      601 ggtgctcgtg atgacgaggg gtatcaaaac ggctgcgagt ctggtgcgta acatgatggt
      661 ctccttgcgt tgcccgtcgc gatggtcatt ccaccggttc ccgaacatgc gcacgcgccg
      721 cgcgaggggc agttagtcca acgccgcaaa cgttcggtag gcggcgttcg gctggcaggc
      781 gactcgttcg ttaccgcggc agcagaccgt aaagcagtcg gccgccgatc acgtccaggg
      841 cgggggcgtt ctcaccgcct tcgggaatga tgagatcggc gtgttgcttg ctgggttcga
      901 cgtgcaggcg atgcatcgcg cgcacggtgc ccatgtactg cgcttcgatc gaccggatgt
      961 cgcgaccgcg ttccagcatg tctcgcttga tgcgccggat gagccggacg tcgtcgtcgg
     1021 tgtcgacgta cagccggagg tcgaacacgt cgcgcagtgt gggcaccgcc agcagcagga
     1081 tcccctcgac cacaatcacc gggcaggggt cgatgcggcg cacgctggac ttgcgcgtgt
     1141 gcgtcgtgaa gtcgtactcc gggcacgcaa cgggttggcc ggccttgagc gcccgcaggt
     1201 gttgcagcag caggtcattc tccagcgcgt cgggttcgtc gaagttcagc ccggcccgct
     1261 gcgccggggt gagcgcggtt cggtcgcggt agtacgcgtc ctgctggatc agcgcggcct
     1321 ggccggcagg tagggcctcg atgatgcggc gcgcgacggt ggtcttcccg ctggcggtgc
     1381 cgccggcgat gccgatcagg gtgaagctgc gagagacagg gacgctcatt ggtcgttcag
     1441 tcattcaccc tggccgatct ttgatttcgg ggtcttttcg tcgcgcccat cttccggtca
     1501 acaacacgac agcgcgattg cagaatatcg gaggtgtggg cgaatagtca gtgctcagtc
     1561 agcgacccgc gcgcgtacga tgtccccgaa gtcagtccga acggacggta ttacgtccag
     1621 aaatatgcga actggacgcc cctgtccctg tggccggtcg gccccggcca gggaaccgac
     1681 ggcctcggcg gctacgttcc ctgttcgatc gcggcgggga attgctgcac gagcggttcg
     1741 ttcctcacag tcgggacgtg cgccccgtgt ggtccggccg g
//
LOCUS       DASWVF010000131         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_283847, whole genome shotgun sequence.
ACCESSION   DASWVF010000131 DASWVF010000000
VERSION     DASWVF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283847"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGD55_02320"
     CDS             complement(<1..223)
                     /locus_tag="VGD55_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869208.1"
                     /translation="MTEHGADAPHVGHGASTDVVGHDSPNGVVTTRGFAPDDDAPPPP
                     LPRVTRRKLPIATLVLALLAVGAAGFFVGV"
     gene            complement(275..1510)
                     /locus_tag="VGD55_02325"
     CDS             complement(275..1510)
                     /locus_tag="VGD55_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869209.1"
                     /translation="MIRTESIRIALSGILHNRMRSALTMLGMLIGVGSVIMLVAVGTG
                     SSQKVQKQIEGLGTNLLQINKQGGFGGFGGRATTGTQSKAANLTAADVTALEDKTQNP
                     DLVAVAPVTMASSVTAVYQGASYSPSSFVGTTPSYEQIKDYQVEEGTFFTAADESQHN
                     RVAVIGETVATNLFGTADPIGNNVQFNGSSYQIIGLLKPKGTNGIQDQDDVVMAPLTA
                     VQDTLTGYSASYSSIDVEAASPSKQADAQAEVTATLETTHDIAAGGTDDFRILNQASL
                     LATSTSSNKVFTTLLGAVAAISLLVGGIGVMNIMLVTVTERTREIGIRKAIGAKRSDI
                     MGQFLVEAVLLSALGGVAGVIAGIGGSHFKIIGIQPVVADYSVVLAFAVSVVTGLFFG
                     LYPASRAARLLPIEALRYE"
     gene            complement(1510..2271)
                     /locus_tag="VGD55_02330"
     CDS             complement(1510..2271)
                     /locus_tag="VGD55_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869210.1"
                     /translation="MSAPAAVPVIKLWQLTKTYGDGEAAVHALRGVSLAVYEGDYVAI
                     MGASGSGKSTLMSIIGCLDVQTSGRYLLDGVDVELLNENELSEIRNRKIGFVFQSFNL
                     LPGSTALQNVELPLTYAGVRSSERRRRATEALDAVGLAQRYGHRPAMMSGGERQRVAV
                     ARAIAVHPSLILADEPTGNLDSVATDDVLAIFARLNTEGRTIVMITHENEVAQHAKRV
                     VRVRDGLIIDDTRQAPIEGPPPLHTLTTYAQVGAH"
     gene            complement(2268..>2789)
                     /locus_tag="VGD55_02335"
     CDS             complement(2268..>2789)
                     /locus_tag="VGD55_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY0869211.1"
                     /translation="SKVAVGAPATVTLSAIPSKEFAAHVIEVANSSTVVSNVVEYQVT
                     FQLDNTDPTIKPGMTASVTVTTAKVDGVLNVTSAAVRTAAGTSYVEVMQPDGTQKQVD
                     VVVGLKGDTTTEITGAVKAGDVVTLPVSTVARSTTTTGTTTRTGTTGLGGGAAVFGGG
                     GGFAVPGGGGGRG"
BASE COUNT          469 a          925 c          890 g          505 t
ORIGIN      
        1 tgaccccgac gaagaagccg gccgcgccaa ccgcgagcag tgcgagcacg agggtggcaa
       61 tcggcaactt gcgacgagtg actctcggca gcggcggtgg cggcgcatcg tcatcgggcg
      121 cgaagccacg cgtcgtgacg acgccgttcg gcgagtcgtg gccgacgacg tcggtgctcg
      181 cgccgtggcc gacgtgtggg gcgtcagcgc cgtgctctgt catgagattc tccggtggtt
      241 ggtcatggct ccgttgttgg tcatagcgct gccgttactc gtatcgaagt gcttcgatgg
      301 gcagcagccg ggcggcccgg cttgctgggt agaggccgaa gaacaatccg gtcacgaccg
      361 acacggcgaa cgccagaaca accgagtagt cggcgacgac gggctggatg ccgatgattt
      421 taaagtgact gccaccgatg ccggcaatca cgccggccac gccgccgagc gccgacaaca
      481 gcaccgcctc gaccaaaaac tggcccatga tgtccgaccg cttcgcgccg atggccttgc
      541 ggatgccgat ctcacgggtc cgctccgtga cggtgaccag catgatgttc atgacgccga
      601 taccgccgac gagaaggctg atcgccgcga ccgcgccgag cagcgtcgtg aacaccttgt
      661 tcgacgacgt cgacgtcgcg agcaggctgg cctggttcaa gatgcggaag tcgtctgtac
      721 cgccggcggc gatgtcgtgc gtggtctcga gggtcgccgt gacctcggcc tgcgcgtcgg
      781 cttgcttcga aggtgacgcg gcctcgacgt cgatgctcga gtacgaagcg ctgtagccgg
      841 tgagggtgtc ctgaactgcc gtcagcggcg ccatcaccac gtcgtcctga tcctgaatgc
      901 cgttcgtgcc cttcggcttc agcaaaccga tgatctgata gctcgagccg ttgaactgca
      961 cgttgttgcc gatcgggtcg gcggtgccga acaggttcgt cgcaactgtc tctccgatca
     1021 ccgcgaccct gttgtgctgg ctctcgtcgg cggcggtgaa gaacgtgcct tcctcgacct
     1081 ggtagtcctt gatctgctca tagctcggcg tcgtgccaac gaacgacgac ggtgagtacg
     1141 aagcaccctg gtagaccgca gtcaccgagc tcgccatcgt cacaggagcg acggcgacga
     1201 gatcagggtt ctgcgtcttg tcttccaagg ccgtgacatc tgcggccgtg aggttcgccg
     1261 ctttcgattg ggtaccggtg gtcgctcgtc cgccgaaccc gccgaagcca ccctgcttgt
     1321 tgatctggag caggttggta ccgagacctt cgatctgctt ctgcaccttc tgcgacgacc
     1381 cggtgccgac cgcgaccagc atgatgaccg aaccgacacc gataagcatg ccgagcatcg
     1441 tgagcgccga ccgcatgcgg ttgtgcagga tcccgctcaa ggcgatgcgg atgctttcgg
     1501 tgcgaatcat cagtgcgccc cgacctgcgc gtacgtcgtc aacgtgtgca gcggcggcgg
     1561 cccttcaatc ggcgcctgcc gggtgtcatc gatgatgagg ccgtccctga cccgcacgac
     1621 ccgcttcgcg tgttgggcca cttcgttctc gtgagtaatc atgacgatcg tgcggccctc
     1681 ggtattgagc cgagcgaaaa tcgcgaggac gtcgtcggtg gccacggagt cgaggtttcc
     1741 cgtcggctcg tccgcgagga tcagggacgg atgcaccgcg atcgcccgcg ccacggcgac
     1801 ccgctggcgt tcaccacctg acatcattgc cggccggtgc ccgtagcgct gggcgaggcc
     1861 gacggcgtcc aatgcctccg ttgcgcgacg gcgacgctcg ctcgaccgaa cgcctgcgta
     1921 cgtgagcggc aactcgacgt tctgcagcgc cgtcgaccct ggcagcaggt tgaagctctg
     1981 gaagacgaac ccgatcttgc ggttgcggat ctccgacaac tcgttctcgt tcagcagttc
     2041 gacgtcgacc ccgtcaagca ggtagcggcc agaggtctgc acgtcgaggc agccgatgat
     2101 gctcatcaag gtgctcttgc ctgaccccga cgcacccatg atggcgacgt agtcaccctc
     2161 gtagacagcg aggctgacac cgcgcagcgc gtgcacggca gcctcgccgt cgccgtaggt
     2221 cttggtcagc tgccacaact tgatgacggg cacggccgcg ggagcactca tccgcgaccg
     2281 ccaccgccgc cagggacggc gaagccgccg ccaccaccga acacagccgc tccgccacca
     2341 agcccggtgg tgcccgtgcg ggtcgtggta cctgtcgtgg tcgtgctgcg cgccacggtg
     2401 gaaacgggca acgtcacaac atcaccggcc ttcacggctc cggtgatctc cgtcgtggtg
     2461 tcacccttca agccgacaac cacatcaacc tgcttctgag tgccgtccgg ttgcatcacc
     2521 tcgacgtagg acgtcccggc cgccgtgcgc accgccgccg acgtcacatt gagaacgccg
     2581 tcgacctttg ccgtggtcac cgtcaccgat gccgtcatgc ccggcttgat cgtcggatcg
     2641 gtgttgtcca actggaacgt cacctggtac tcgacaacgt tgctgacgac cgtcgacgag
     2701 ttcgccacct cgatgacgtg cgcagcgaac tccttgctcg gtatcgcgct gagggtgacc
     2761 gtcgccggcg cgccgacggc gaccttcga
//
LOCUS       DASWVF010000132         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_309255, whole genome shotgun sequence.
ACCESSION   DASWVF010000132 DASWVF010000000
VERSION     DASWVF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309255"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..2199
                     /locus_tag="VGD55_02340"
     CDS             172..2199
                     /locus_tag="VGD55_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869212.1"
                     /translation="MRIAFVCRDLANARLTGPGARAFSTACAMADAGHDVHLVSESLS
                     AHRARGLAAGRGPHWTRVRETRPHHRYFTEQHAYADRIYDTLQKLTGSEPFDVIEFSS
                     AAAEALTTVRAKRLLGEFSGTSLVVRMGTELEGDRAATHIAPLDLERSVDDFARTYIA
                     AHADTLLTDTLLTDALLTDAATVDVSDCSVASRIRFAPFVADPLGAPVLRIPEFGAAL
                     TSWHLGELRPGSGVATFIDAARIVLASEPRAKFIIAGDDTASDPFGRSYWDFCKRQLS
                     GELLDAVTFAGPLVDARETLPKPGSQCVIASATSDATGAAVLAMGRGCIVTVREGSAA
                     AAFVDADVSASVVQSGDAVALAAALLRVMRDPARAVALSAGGTARVAAHYQPRHVAAS
                     LIRAYRSGATHTAVELSPEPSELVSVVIPLYNQGSFLLGAVESVRAAGYPNVEIVVVD
                     DGSTEPETISIFNDLAGVTKVRQVNAGLSAARNAGIAAAAGRYVVPLDSDDLLPAGFL
                     RPAVDALRRNPELGYVAGYLRYFGLLDYTHVPAGYIPHLSLVVNTHARATALFTRESL
                     MAVGGYDVQLPAFEDWDLHVRLALAGYTSDIVPLEGHRYRRHAESMTFSSSNAMRLEL
                     LQHLMAKHLGSIDHEHACDLLILMANLWKTGYEPSASVRLQQEAKQPLVPS"
     gene            complement(2207..>2617)
                     /locus_tag="VGD55_02345"
     CDS             complement(2207..>2617)
                     /locus_tag="VGD55_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0869213.1"
                     /translation="SLFIIRNGPSQYREEVSMIVGYDHVQVAIPSGAEDVAREYYGGL
                     LGMTELPKPAPLAARGGCWFTAGAAVLHLGVEADFRPARKAHPAFVVEDLDALERTLQ
                     AAGFGTTSANDEIPDLRRFHTFDPFGNRLEFQQA"
BASE COUNT          447 a          842 c          841 g          487 t
ORIGIN      
        1 ctgcgtcggg ccgaaggtca gcaccttggt gtttctgtgc ccattgcccc tgaaacgctg
       61 tgcattccag cagaaaacgg cgatcatgcg acgaaagaac gacatgttga aatctcgcca
      121 cgacggctgt cgacccgatc gttgacatga ggatcgcgac gggagatgcc ggtgcgcatc
      181 gcctttgttt gtcgcgacct tgcgaacgct cgcctcaccg gtcccggagc acgtgccttc
      241 tcgacggcgt gcgcgatggc cgatgcgggg cacgacgtgc atctcgtcag tgagagtctt
      301 tctgcgcatc gggctcgcgg acttgcggcc gggcgtgggc cgcattggac ccgggttcgc
      361 gaaacacgac cccatcatcg ttacttcacc gaacagcacg cgtatgcaga tcgcatctac
      421 gacaccctcc agaagctgac cggcagtgaa ccgttcgacg tgatcgagtt cagctcggcg
      481 gcagcagaag ctctgacgac agtgcgtgcg aagcggctgc tgggtgagtt ctccggcacc
      541 tcacttgtgg tgcggatggg caccgaactc gaaggcgacc gtgcggcaac gcacatcgcc
      601 cccctcgatc tagaacggtc ggtcgacgac ttcgctcgca cgtacatcgc ggcgcacgcc
      661 gacaccttgt tgaccgacac tttgttaacc gacgctctgt tgaccgacgc tgcgactgtc
      721 gatgtgtcgg actgctcggt cgcgtctcgc attcggttcg cacccttcgt agccgaccca
      781 ctcggcgcgc cggtgctccg cattccagag ttcggtgcgg cgctcacgag ttggcaccta
      841 ggtgaactgc gacccggatc tggggtcgcc accttcatcg acgccgcacg gatcgttctc
      901 gcgagcgaac cgcgcgcgaa gttcatcatc gcaggagacg acacggcgtc cgatccgttt
      961 ggccggtcgt actgggattt ctgcaagcgt cagctgtcgg gcgaactgct ggatgccgtc
     1021 acgttcgccg gcccgttggt tgacgctcgc gagacgttgc cgaagccagg gagccaatgc
     1081 gtcattgcaa gcgcgacgtc cgatgcgacg ggcgcggcag tgctcgcgat gggtcgcggt
     1141 tgcatcgtca ccgttcgtga aggcagcgca gcggcggcgt tcgtcgacgc cgacgtgagt
     1201 gcgagcgtcg tgcagtcagg tgatgcggtc gcgctcgcag cagctctgct tcgggtgatg
     1261 cgggaccccg caagagcggt cgcgttgtcg gccggcggca cggctcgcgt cgctgcgcac
     1321 taccagccac ggcacgttgc ggcgtccctc attcgcgctt atcgatctgg cgcaacgcac
     1381 accgcggtcg agctgtcgcc ggagccaagc gaactcgtat cggtcgtgat cccgctttac
     1441 aaccagggca gtttcctgct cggcgcggtc gagtcggtgc gcgcagctgg atatccgaac
     1501 gtcgagatcg tcgtcgtcga cgacggatcg accgaacccg agacgatcag catcttcaac
     1561 gaccttgcag gggtcacgaa ggttcgccag gtgaacgccg gactgtccgc ggcccgcaac
     1621 gcgggcatcg ccgccgccgc tggtcgctac gtcgttccgt tggactccga tgacctgcta
     1681 cctgccggat tccttcgacc cgcggtcgac gcgttgcggc gcaaccctga gctgggttac
     1741 gtcgccgggt atctgcgcta cttcggattg ctcgactaca cccacgtgcc ggcgggctac
     1801 atccctcacc tgagcctcgt cgtcaatacg cacgcacgcg caacagcgtt gttcacccgc
     1861 gaatcgctga tggccgttgg cggctacgac gtgcagttgc cagcgttcga ggactgggat
     1921 ctgcacgtgc ggctcgcgct cgccggctac acctccgaca tcgttccgct ggaggggcac
     1981 cgctaccgaa ggcacgccga atcgatgacg ttcagcagca gcaacgcgat gcggctcgag
     2041 ctcttgcaac atctgatggc gaagcacctc ggcagcatcg accatgagca tgcgtgcgat
     2101 ctgctcatct tgatggcgaa cctatggaag accgggtatg agccgagtgc ttcggtacgg
     2161 ctgcagcaag aggcgaagca accgttggtc ccgagctgag gtcaggtcac gcctgctgga
     2221 actcgaggcg gttcccgaac gggtcgaagg tgtggaagcg gcgcaggtca ggtatctcgt
     2281 cgttggcgct cgtggtaccg aagcccgcgg cttggagcgt tcgctccagg gcatcgaggt
     2341 cttccacgac gaacgccgga tgcgccttgc gagcaggtcg gaaatcggcc tcaacaccga
     2401 gatgcaggac ggcggctccg gccgtgaacc agcaaccacc ccgtgcggcg agcggggccg
     2461 gctttggcag ctcggtcatg ccgagcaacc ccccgtaata ctcgcgggcc acatcttccg
     2521 cgccgctggg aatcgcgact tgcacgtggt cgtacccgac gatcatcgac acttcctctc
     2581 ggtattgcga cgggccgttg cggatgatga agaggct
//
LOCUS       DASWVF010000133         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_462317, whole genome shotgun sequence.
ACCESSION   DASWVF010000133 DASWVF010000000
VERSION     DASWVF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462317"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VGD55_02350"
     CDS             <1..233
                     /locus_tag="VGD55_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HEY0869214.1"
                     /translation="RLRGQIEAIERGIEAGDDCAKVLHLIAASRGAINSLMTEVLEGH
                     VREHLIDPDKERSTARGKAAQQLIDVLHSYLS"
     gene            271..2118
                     /locus_tag="VGD55_02355"
     CDS             271..2118
                     /locus_tag="VGD55_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0869215.1"
                     /translation="MTTALCALAVLTGWLLLRGRLHAAGVAVLITGYVVGGYRQARDG
                     LLTLIRDRELDVDLLMVVAAIGAAAIGEWFDGALLIFIFALSGALEGYASARTTRDIT
                     ALMALTPEEAILLRDGAEQVVPASDLKVDDRIVIRPGARIPADGAVVEGTSAVNQAPI
                     TGESVPVEKSFGSDVFAGSINGHGALYVNVTRTPGDTLLARIISLVREAQANRPRAQL
                     FIERFERGYAKVVVLGAIFLGSIPAAVGWWTGREAIYRAMIFLVVASPCALAAAMMPT
                     LLSALSNAARNGILFKGSAFIEALGKIDAIAFDKTGTLTSGHPVVAEVVSTSDTPPEV
                     LLAHAASIESASEHPIGRAIVAEAARRRLTIVAVTDFQAVVGSGARARLSGAEWSVGR
                     PTLFSMADDVNRSVALLESQGHTVVMVGSGTCLGYIALRDKVRPEAGAAIAFLRHLGI
                     KPIVGLSGDSRATADAVAAAVGVDEMHAGLYPQDKVRKVKELASSHGAVAMVGDGVND
                     APALAVAHVGIAMGGIGTDVALETADVVLSRDDVSMVPYAVALGRRSLRIIRQNLAIA
                     LTVIVALVLSDLFGLITLPWGVVGHEGSTLLVTLNGLRLLRRLPRPVVT"
BASE COUNT          534 a         1064 c         1101 g          581 t
ORIGIN      
        1 gacgactgcg tggacaaatc gaggcgatcg aacgcggaat cgaggcgggc gacgactgcg
       61 ccaaggtcct ccacctgatc gcggcctccc gcggcgccat caactcgctc atgaccgagg
      121 tgctcgaggg ccacgttcga gagcacctga tcgaccctga caaggaacga tcgaccgcgc
      181 gtgggaaggc ggctcagcag ctgatcgacg ttcttcattc ctacctgagc tgaggtcgag
      241 atgcgtgctt ggaaagacca cgcggaagcg gtgacgaccg ccctttgtgc gctcgccgtt
      301 ctcaccggat ggctgcttct gcgcggaaga ctccacgccg ccggagtcgc ggtgctcatc
      361 accgggtacg tcgtgggcgg ctaccgccag gcgcgcgatg gcctgctcac gctcatccgc
      421 gaccgcgagc tggacgtcga ccttctgatg gtcgtcgcgg ccatcggcgc tgccgccatc
      481 ggcgagtggt tcgacggcgc cctgctgatc ttcatcttcg cgctgagcgg cgcgctcgag
      541 ggttacgcga gcgcgcgaac gacgcgcgac atcactgccc tcatggcgct cacgcccgag
      601 gaggccattc tcctgcgcga cggggctgag caggtcgtgc ccgcgtccga tctaaaggtg
      661 gacgaccgga tcgtgatccg ccccggagcc cggattcccg cggacggcgc cgttgttgaa
      721 ggaacgtcgg ccgtgaacca agcgcccatc accggagagt ccgtaccggt cgagaagagc
      781 ttcggctcgg acgtgttcgc cggttcgatc aacggtcacg gtgctctcta cgtgaatgtc
      841 acgcgcacgc cgggggatac tcttctggcc cggatcatct cgctcgtgag ggaggcccaa
      901 gccaaccggc cgcgggcgca gctcttcatc gaaaggttcg agcgtggcta cgcgaaggtc
      961 gtcgtgctcg gagcgatctt cctcgggagc attccggcgg cggttggatg gtggaccggc
     1021 cgcgaagcca tctaccgcgc gatgatcttc ctcgtggtgg cgtcgccgtg tgccctcgcc
     1081 gctgcgatga tgcccaccct gctctcggcg ctgtccaacg cggcccgaaa cgggatcctt
     1141 ttcaagggga gcgcgttcat cgaagccctg ggcaagatcg acgccatcgc gttcgacaag
     1201 accggcaccc tcacctcggg gcatccggta gtcgccgagg tggtctccac gagcgacacc
     1261 ccgccggagg tcctgcttgc acacgctgct tccatcgaga gtgcgtcgga gcaccccata
     1321 ggccgcgcca tcgtggcgga ggcggcgcgc cgacgactga caatcgtcgc cgtcacggat
     1381 ttccaggcag tcgtcggttc cggtgcccga gcccgtcttt cgggcgcgga gtggtcagtc
     1441 ggccgcccca ccctcttctc gatggccgat gacgtgaacc gcagtgtcgc actactggag
     1501 agccaggggc atacagtggt tatggtcgga agcggcactt gcctcgggta catcgcgctc
     1561 agggacaagg tccgaccgga ggccggtgcc gccatcgcat ttctcaggca cctcggaatc
     1621 aaaccgatcg tgggcctgag cggcgacagc cgagccaccg cagacgcggt cgccgcagcg
     1681 gtcggcgtcg acgagatgca cgccggtcta tatccacagg acaaggtgcg caaggtgaag
     1741 gagctcgcgt caagccacgg cgccgtcgcg atggtcgggg acggcgtcaa cgacgcccct
     1801 gctctcgcgg tggcacacgt cgggattgcg atgggcggaa tcggaacgga cgtggcgctc
     1861 gaaaccgctg acgtcgtcct gagcagggac gacgtcagca tggtccctta cgccgtggcg
     1921 ctcggtcgtc ggtcgctgcg gattatccgc cagaacctcg cgatcgcttt gacggtgatc
     1981 gtggcgctcg ttctgtcgga cctgttcggg ctcatcaccc tgccctgggg tgtcgtggga
     2041 cacgaaggca gcaccctgct agtaacgctc aacggactcc ggctccttcg aaggctcccg
     2101 cgtcccgtgg tgacgtgagt ccggagatgg gcgctcgacc agtccggacc acaatgccca
     2161 cctcgccgcc acaagcctct gtgaaccact ccgtgcggcg tttggtcaca tatttcgtcg
     2221 tgccgcccct ggtgggcttg tgcgcctgct tcgtcgcggc ggccttcgcc ttcgccgtag
     2281 cggtcggtgt taggaacggc attttcgggg agccaccacc acgactggct gacgactgaa
     2341 gatcttctca cccggttgct gatgacgagg tgcatatggc cgccgcgggc gtccgcaagg
     2401 gacgcatcgg gttgcgcctg aagcgcgtct cgctggtgcg agggagacag ctgcgtcgca
     2461 ggccaggcca tgtcgaggcc ggcgccagcc ggtatctctt cggatggtca cgatccccgt
     2521 cggcctcggt ctcgccctca gcaagcgcag ctgctgctga ctcccgggcg ctatgtcgcg
     2581 cccacgcgga ccggtgacat catggctgtt ccgtggggcc tggctttgac cgtttcggag
     2641 tattcagccg ccgcttcgat tcggctcgcc aatagacgca gtgaccgaaa ccatacctgt
     2701 gccccgcacg caaccaaatc agcaccccaa ccagtcccgt caggctgacc caggtctgac
     2761 cttccttgaa cggctaacgg ttggggtggc ggccgttcca atagcttgtc tcgctgtcgc
     2821 gttcgtcgtc ggtggctact gttacgcggt cggcgtcggc atcagagacg gcttcttggg
     2881 agggccaagg ccggcggaag ccgaggattg atcgcgaccc gttctagttg catccgtcac
     2941 ggcaccggct cgacacaacc gcgcatcgtg caatggcacc gtgcgactcg cacgagagaa
     3001 tcgtgttcct ccgcgacttc gatcaccgtg ttgcggggcg ggctcaagtg agccgacgga
     3061 gagtcgatgt tggggcgaca acaggtccgg cgcaggcgga tccggatcgc gtcagctttg
     3121 gacaggagaa ggcgaatgac agacgcccgg aagaagatgg cggtggtgac gatggcggtg
     3181 gcggccggcc tcttgggcgc gggctgtcgc gggcagacgg tcgtcaacac cgatggagag
     3241 ggctccgagg tcgcgacgag cgtcgtcgga ggcgctctga
//
LOCUS       DASWVF010000134         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_3633, whole genome shotgun sequence.
ACCESSION   DASWVF010000134 DASWVF010000000
VERSION     DASWVF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGD55_02360"
     CDS             <1..546
                     /locus_tag="VGD55_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0869216.1"
                     /translation="LLGGGEDDAQVAAARSLRADGGLARAAAKGAVIFGVCAGYQLLG
                     TVFPGSDGAPEPGVELLDVTTDRLPRRAVGELLAAPCSAGLELPLTGYENHAGSTHLG
                     SSAAPLARVEAGIGNGSTFEGAVQGRMLGTYLHGPALARNPELADLLLGWVVGEALTP
                     WVQADVDALRAERLGAVRPRK"
     gene            631..1233
                     /locus_tag="VGD55_02365"
                     /pseudo
     CDS             631..1233
                     /locus_tag="VGD55_02365"
                     /inference="COORDINATES: protein motif:HMM:NF042501.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
     gene            1314..>1609
                     /locus_tag="VGD55_02370"
     CDS             1314..>1609
                     /locus_tag="VGD55_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY0869217.1"
                     /translation="MVDTSLSGALLVASPTLADPNFARSVVLLLDHDSEGALGVILNR
                     PSEIATRDAVPLPRLPGWADLVSEPAVVHVGGPVTPEAVVCLGRIRGDRRQRQGV"
BASE COUNT          229 a          528 c          546 g          306 t
ORIGIN      
        1 ttgcttggtg gcggggagga cgacgcccag gttgccgccg cccgctcgct gcgcgccgac
       61 ggaggcctcg cccgcgcggc tgccaaaggt gcggtgatct tcggtgtatg cgcgggttac
      121 cagctgctcg gcaccgtgtt ccccggatcc gacggcgcac cggagccggg ggtcgaactg
      181 ctcgatgtga caaccgatcg gctgccgcgt cgggcggtcg gcgaactcct cgcggcgccg
      241 tgctcggcgg gcctagagct tccgctgacg ggatatgaga accacgccgg atcgacccat
      301 ctcggcagca gtgcggcgcc actcgcgcgg gtcgaggcag ggatcggcaa cggcagcacc
      361 ttcgagggcg cggtccaagg ccggatgctc ggtacctacc tgcatggtcc ggccctggca
      421 cgaaacccgg aactggccga cttgctgctg ggatgggtcg tgggggaggc gctcacgccc
      481 tgggtgcagg cagacgtcga tgcactgcgc gccgagcggc ttggcgcagt gcgtccgcgc
      541 aaataacagg aaactcacag gcagatcggg aagaaggtcg tgctggcgag gagttgcagc
      601 ttttcgtaag gtcaacgcga ggaggtgcgt atggtcagtt tgtcgaccct cgcgccgcag
      661 cggatttcgg ctcgcggctg gcttccaaac tggcgcacaa cgccgttcac gatcgtctac
      721 gtaacgttga ttaccgtcgg cagcgtggtg cggagcctcc tcgatgacgg cgaccatgac
      781 gcagtgcttc gcacatcgag taccgatgtg caccacttgt cgacccatcc gatcttcgtg
      841 cttgtcacca gcgctttgtg ggtcgacggc atcgtcgacg cactccttgc cgtactcgtg
      901 cttggcgtgc tcgcgacgat cctcgagcgg cgtgtcggca cccgctgggt ggtagctatt
      961 ttcgcctcag gccacgtggt cgcgaccctg ctcaccgaag gcgccgttgc cgtcggcgtc
     1021 cacgctggga tgttgccttc aagtgctgtg tcgcgcatcg acgtcggcgc cagctatggc
     1081 ctggctgcac ttcttggtgc gacggcgggg ttgctgccca gacgagttcg tacgatcgga
     1141 gtcttcgccg cctggatcta cctcggctgg ccgatcgcca accgcttcga catgacgtcg
     1201 tgggggcacc tgatcgcgtt ggccgtcggc gtgtgctggt ggccctatct gccgcggctc
     1261 gcgactcttg ccccgttgca gcggcacgac gaattcatcg gcatgatgga gcgatggtcg
     1321 acacctcact ttctggagcg cttctcgttg cgtcccccac tcttgccgac ccgaacttcg
     1381 cacgcagcgt tgtcctcctc ctcgaccacg acagcgaagg cgcgctcggc gtcatcctca
     1441 accggcccag tgagatcgcg acccgcgacg cagttccgct cccgcgtctg cccgggtggg
     1501 ccgacctcgt aagcgagccg gcggtggtcc acgtcggcgg tcctgtgacc cccgaggcgg
     1561 tcgtgtgcct gggtcgcatt cgcggtgatc gccggcaacg gcagggtgt
//
LOCUS       DASWVF010000135         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_385891, whole genome shotgun sequence.
ACCESSION   DASWVF010000135 DASWVF010000000
VERSION     DASWVF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385891"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGD55_02375"
     CDS             <1..625
                     /locus_tag="VGD55_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869218.1"
                     /translation="RAASVVTGPDAVPTEIHIVASRSKGAKQVVRDVQSLAMAGYDID
                     IDHRIVSVVQFDDDIDGVQADDNLHTIIRMPDTAPAIDALPRPVIAAITIRTAGSEAD
                     ASVVVRTGDATYDGRSIGPSTMSHRHTLIARATLDAVSELLGLPAEVEFVTVSTMGNR
                     RMAACVVQVAVPGIGELVLTGSALVRSDEADAVARAVLDALNRRLAG"
     gene            complement(652..966)
                     /gene="rsrA"
                     /locus_tag="VGD55_02380"
     CDS             complement(652..966)
                     /gene="rsrA"
                     /locus_tag="VGD55_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEY0869219.1"
                     /translation="MSCGKHHETPCTQVLDQVYTYLDGEIDVAECDKIRVHLEECGPC
                     LRQYDLDQAVKALVARSCGCDLASAELRSKVLARIRQVQVEISQGGVTSVEVTQTVYR
                     TD"
     gene            complement(963..>1642)
                     /locus_tag="VGD55_02385"
     CDS             complement(963..>1642)
                     /locus_tag="VGD55_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0869220.1"
                     /translation="GFQRPVMMDTEEVGPVPETLEQRDERFEQDVLPFLDQLYSAGLR
                     MTRNPADAEDLVQETFAKAFAAFHQFETGTNLKAWLYRILTNTFINSYRKKQREPKQT
                     GGDELEDWQLARSAEHTSAGLRSAETEALDHLPDSDVKEALQALPEEFRVAVYLADVE
                     GFAYKEIADIMGTPIGTVMSRLHRGRRALRASLEDYARERGVVRGPIQEVGVVGVVGA
                     VEGGDGQ"
BASE COUNT          286 a          535 c          523 g          298 t
ORIGIN      
        1 ccgggcggcc agcgtggtga ccgggcccga cgccgtcccg accgagattc acatcgtcgc
       61 cagccgcagc aagggcgcga agcaggtggt gcgggacgtg cagtcgctgg cgatggcggg
      121 ttacgacatc gacatcgacc accgcatcgt gagcgtggtg cagttcgacg acgacatcga
      181 cggggttcag gccgacgaca acctgcacac gatcatccgg atgcctgaca ccgcgccggc
      241 gatcgacgcg ttgcctcgcc cggtgatcgc cgccatcacc atccgcacgg cgggctcgga
      301 agccgatgca tctgtagtcg tccgcactgg cgatgccaca tacgacgggc ggtcgatcgg
      361 gccgtcgacg atgagccacc gacacacact gatcgcgcga gcgacactcg acgccgtcag
      421 tgaactcctc ggattgcctg cagaggtgga attcgtcacc gtgtcgacga tgggcaaccg
      481 gcgcatggcg gcctgcgtcg tgcaggtcgc cgtgcctggg attggtgaac ttgtgttgac
      541 cggctcggcc ttggtgcgca gtgacgaggc ggacgccgtc gcgcgcgcgg tgctggatgc
      601 actcaaccga aggttggccg gctagctgcg taccgaaggt tggccggcta gtcagtcagt
      661 gcggtagacg gtctgggtga cctcgaccga ggtgacgccg ccctgtgaaa tctcgacctg
      721 cacctgtctg atgcgcgcca gtaccttgct gcgcaactca gcggacgcca gatcgcaacc
      781 gcagctgcgg gcgaccaagg ctttcaccgc ctggtcgagg tcgtattgac gcaggcacgg
      841 gccgcactcc tcgagatgga cgcggatctt atcgcactca gcgacatcga tctcgccgtc
      901 gaggtaggta taaacctgat cgagaacctg cgtgcatggg gtctcgtggt gtttgccgca
      961 gctcattgac cgtcgccgcc ttcaactgca ccgaccacgc cgacgactcc gacttcctgg
     1021 atgggcccgc gcaccacacc gcgctcgcgg gcgtagtcct ccaaggatgc cctcaacgct
     1081 cgccggccgc gatgcaatcg cgacatcacc gtgccaatgg gtgtgcccat gatgtcggcg
     1141 atctccttgt aggcgaagcc ttcgacgtcc gccaagtaga ccgcgactct gaactcttcg
     1201 ggaagagcct gcagcgcctc tttcacatcg gagtcgggca ggtggtccag ggcctcggtc
     1261 tcggctgacc tcaacccagc cgacgtgtgc tccgccgagc gggcgagctg ccagtcctcg
     1321 agctcgtcgc cgccggtctg cttgggctcg cgttgcttct tgcggtagct gttgatgaac
     1381 gtgttcgtga ggatccggta cagccacgcc ttgaggttgg taccggtctc gaactgatga
     1441 aacgcggcaa acgccttggc gaacgtctcc tgcacgagat cttcggcatc cgctgggttg
     1501 cgggtcatcc ggagcccggc ggaatacagc tgatccagga acggcagaac atcctgctca
     1561 aaccgttcat cgcgctgctc aagtgtctcc gggacgggac ccacctcctc ggtatccatc
     1621 atgaccggcc tttgaaaacc at
//
LOCUS       DASWVF010000136         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_79927, whole genome shotgun sequence.
ACCESSION   DASWVF010000136 DASWVF010000000
VERSION     DASWVF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79927"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGD55_02390"
     CDS             complement(<1..182)
                     /locus_tag="VGD55_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869221.1"
                     /translation="MIKGWADADGLAFAAELEVEPAVDEPGVDEPGVDEPAADELAAA
                     GRGVWGVWGTHSTLRR"
     gene            214..1551
                     /locus_tag="VGD55_02395"
     CDS             214..1551
                     /locus_tag="VGD55_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0869222.1"
                     /translation="MQKPVKVEIRDNLLVRLKSGEPRFPGIVGFDETVLPHVERALLA
                     GHDFVLLGERGQGKTRLIRTISGLLDEWTPVVDGCEINDHPYAPVCTRCRRLADELGD
                     DLPVDWLNRDQRYGEKLATPDTSVGDLIGDVDPIKVAEGRTLGDPETVHYGLVPRFNR
                     GIFSVNELPDLAERIQVSLLNVLEERDVQIRGYALRLPLDVLLIASANPEDYTNRGRI
                     ITPLKDRFGAEVHTHYPLRLTDEIDLITQEADLSADVPGYLLDVVARFTRLVRESTAV
                     DQRSGVSARFAVAGAETVAASALRRAALTGEADAVARVCDLPGIVDSLQGKVEFEVSE
                     EGRERDVLVHLLRRAVAETFRARLGSADLSGLLGRFDEGATVETGELVSAADLLKRVG
                     QVPGLAKVLERLGDDGGEIPGRAAAAIEFALEGLYLTRRLSKDEVPGRTVYGG"
BASE COUNT          274 a          586 c          545 g          269 t
ORIGIN      
        1 tcgcgacgta gggtcgagtg cgtgccccag actccccaga ctccgcgtcc agcggcggcc
       61 agctcgtcgg ccgccggttc atcgacaccc ggctcgtcga cacccggttc atcgacagct
      121 ggctcgacct ccagctctgc cgcgaacgcc agcccatcgg cgtccgccca gcccttgatc
      181 actacgctcg cggcgctgcg tgcctccggc catgtgcaga aaccggtaaa agtcgagatc
      241 cgcgacaacc tgctcgtccg gctgaagtca ggcgagccgc gctttcccgg catcgtcggc
      301 ttcgacgaga ccgtgctgcc gcacgtcgaa cgcgcgctgc tcgcgggcca cgacttcgta
      361 ctgctcggcg agcgcggcca aggcaagacc cggctgatcc gcacgatcag cggcctgctc
      421 gacgagtgga cgcccgttgt cgacggctgc gagatcaacg accacccgta cgcgccggtg
      481 tgcacccgct gccgccggct cgcggatgaa ctcggcgatg acctgccggt cgactggttg
      541 aaccgcgacc agcggtacgg cgagaagctc gcgactcctg acacgagcgt cggtgacctc
      601 atcggcgacg tcgacccgat caaggtcgcc gagggccgca cactcggcga ccccgagacg
      661 gtgcactacg gactggtccc gcggttcaac cgcggcatct tctccgtgaa cgagctgcca
      721 gaccttgccg agcggatcca ggtgtcgctg ctcaacgtcc tcgaagagcg cgatgtgcag
      781 atccgcggat atgcactgcg gctccccctc gacgtcctgc tcatcgccag tgcgaacccc
      841 gaggactaca cgaaccgcgg tcgcatcatc acgccgctga aggaccgctt cggcgccgag
      901 gtgcacacgc actacccact tcgcctcact gacgagatcg acctgatcac gcaagaggcc
      961 gacctctcgg ctgacgtgcc tggatacctg ctcgacgtcg tcgcgcgttt cacccggctg
     1021 gtgcgcgagt cgaccgccgt tgaccagcgc tccggcgtgt ctgcacgatt cgccgttgct
     1081 ggcgcagaaa ccgttgcggc atcagcactt cgacgggctg cgctgacagg cgaggcggac
     1141 gccgtggcgc gagtctgcga cctccccggc atcgtcgatt cgcttcaagg caaggtcgaa
     1201 ttcgaggtca gcgaggaagg gcgcgagcgt gacgttctcg tgcacctgtt gcgacgcgcc
     1261 gttgcggaga cgttccgtgc tcgactcgga agcgccgacc tcagcgggct tctggggcgc
     1321 ttcgacgagg gtgcgaccgt ggagaccggc gaactggtgt cggctgcgga tctgctgaag
     1381 cgggtcggcc aagtgcccgg cctggcgaag gtgctcgaac gccttggcga tgacggtggc
     1441 gagatccctg gacgcgcggc ggccgcgatc gagttcgcac tcgagggcct gtatttgacc
     1501 cggcgcttga gcaaggacga ggtccccggc cgcacggtgt acggcggatg agttcgccgg
     1561 cgtttcgtta cgggccatgg cacgaaggtc ctgacccact cgcgccgccg tacgacatcg
     1621 cgcaagcact cgacgaactc ggcgacgcgg tactcgacgg cgccaacacc tctg
//
LOCUS       DASWVF010000137         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_283921, whole genome shotgun sequence.
ACCESSION   DASWVF010000137 DASWVF010000000
VERSION     DASWVF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283921"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /gene="metK"
                     /locus_tag="VGD55_02400"
     CDS             complement(<1..1036)
                     /gene="metK"
                     /locus_tag="VGD55_02400"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0869223.1"
                     /translation="MSRRLFTSESVTEGHPDKIADQISDGILDALLKDDPKSRVAVET
                     LITTGQVHVAGEVTTEAWADIPAIVREVILGIGYDSSKKGFDGASCGVSVSIGSQSPD
                     IAQGVDEGYERRHGEGVDDLDRQGAGDQGLMFGYACNDTPELMPLPIALAHRLARRLS
                     QVRKDGVVPYLRPDGKTQVTIEYDGDKPVRLDTVVVSSQHAADIDIDTLLAPDIAEFV
                     VAPEMADLGIDTTRHRLLVNPTGRFEIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFS
                     GKDPSKVDRSAAYAMRWVAKNVVAAGLASRCEVQVAYAIGKAHPVGLFVETFGTGVVP
                     DERIQEAVVQV"
     gene            complement(1097..>1550)
                     /locus_tag="VGD55_02405"
     CDS             complement(1097..>1550)
                     /locus_tag="VGD55_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY0869224.1"
                     /translation="AAVLEAATGADAVVMAAAVADFRPVTMSASKIKKSDDGPAALEL
                     TRTPDVLAELSRADRAGRAAQVIVGFAAETDDVMANAQAKLVRKGADLLVVNEVGSGK
                     GFEVSDNAGVILGADGTATEVPPGPKVALADVVWDLVAIRLNRLPAVS"
BASE COUNT          259 a          530 c          500 g          261 t
ORIGIN      
        1 agacctgcac cacggcttcc tgaattcgct cgtcgggcac cacgccggta ccgaaggtct
       61 cgacgaacaa gccaacgggg tgcgccttgc cgatggcgta cgcgacttgt acctcgcagc
      121 ggctggcgag cccggcggcc acgacgttct tggcaaccca gcgcatcgcg taggcggcag
      181 accggtcgac cttcgagggg tccttaccgg aaaacgcacc gccgccgtgg cgtgccatgc
      241 cgccgtaggt gtcgacgatg atcttgcggc cggtcagacc ggcgtcgccc atcgggccac
      301 cgatctcgaa gcggccagtc gggttcacca gcagccggtg acgggtggtg tcgatgccca
      361 ggtcggccat ctcgggagcc accacgaact cggcgatgtc aggcgcaagc aacgtgtcga
      421 tgtcgatgtc ggcggcgtgc tggctggaga ccacgacggt gtcgaggcgc accggcttgt
      481 cgccgtcgta ctcgatcgtg acctgggtct tgccgtcagg gcgcaggtag ggcacgacgc
      541 cgtctttgcg cacctgcgac agccgccgag ccagtcggtg ggccagcgcg atcggtagcg
      601 gcatcaactc aggagtgtcg ttgcacgcgt acccgaacat gagcccctgg tcgcctgctc
      661 cttggcgatc aaggtcgtcg acgccttcgc cgtggcgacg ctcgtaacct tcgtcgaccc
      721 cctgcgcgat gtcaggcgac tgcgagccga tcgacacaga tacgccacag gatgccccgt
      781 cgaaaccctt cttcgacgag tcgtagccga tgccgaggat cacctcgcgg acgatcgccg
      841 ggatgtctgc ccacgcctcg gtcgtcacct caccggcaac gtgcacctgg cccgtggtga
      901 tcagcgtttc caccgcgacg cggctcttcg ggtcgtcctt tagcagcgca tcgaggatgc
      961 catcgctgat ctgatccgcg atcttgtcgg gatgaccttc ggttacggac tctgacgtga
     1021 acaaacggcg ggacacaagg ctccctcggt tggcacacgc gaagcgcacc aaagacggcg
     1081 gatgacgatc gccagtctaa gacacggcgg gtagtcggtt cagccgaatc gcaacaaggt
     1141 cccagaccac gtcagccagc gcaactttcg gtcctggtgg gacctcggtc gctgtgccgt
     1201 cggcgccgag gatgaccccg gcattgtcgg aaacctcgaa gcccttgccc gagccgacct
     1261 cgttgaccac gagcagatca gcgcccttgc gtacgagctt cgcctgcgca tttgccatca
     1321 cgtcgtcggt ctcagccgcg aacccgacga tgacctgcgc cgccctgccc gcgcggtcgg
     1381 cgcggctgag ttcagcaagc acatcgggag tgcgggtgag ctcgagcgct gccggcccgt
     1441 cgtccgactt cttgatcttg gacgccgaca tggtgaccgg cctgaagtcg gcgaccgccg
     1501 cggccatcac caccgcgtcg gcaccggtgg ccgcctcgag cacggcggcg
//
LOCUS       DASWVF010000138         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_131232, whole genome shotgun sequence.
ACCESSION   DASWVF010000138 DASWVF010000000
VERSION     DASWVF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131232"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VGD55_02410"
     CDS             <1..1336
                     /locus_tag="VGD55_02410"
                     /inference="COORDINATES: protein motif:HMM:NF044554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNase A-like domain-containing protein"
                     /protein_id="HEY0869225.1"
                     /translation="PMAVSAAGRTLESIALDVAKIAGRLRALASGDDSWTGAAAASAH
                     VRTASLPPTLDKANASYAAAGSALGSYARTLADAQDQSSSAIAAADRASAELAAAVSA
                     HAAAAARDASALAAARTAGLPPPASTAPRYEASIDEASAQLKRAVAANDDAHEQQRQA
                     ARIAASALQQASRAGIHNQSWFHHVTHSVGHWASTHWTATLREVSKVGTIVSALAGAA
                     ALILAVAGVAFPPLEAAAAALETVSLVSAVAAGMADTALAATGKASWTSVGVDALSFA
                     PAGVGKLVTKAAPLIREGRLLKPTTVVHASNGEATRAVQASQGSRLAYPGGLGANDAL
                     GGHVLIHVGARDEDLAARRLPAASTFTDRATAEHALAEVQSSSASEIRTWLAVAPQGE
                     RASFGVTFDTPVGRILVRGQRKSIGCVSAVAVLRADRSAPLGYIVLTGFVAP"
     gene            1333..1680
                     /locus_tag="VGD55_02415"
     CDS             1333..1680
                     /locus_tag="VGD55_02415"
                     /inference="COORDINATES: protein motif:HMM:NF036906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HEY0869226.1"
                     /translation="MTRRYFAVQEMFVLYFHEDWRLDADSRLEVATDFLATASPVLID
                     DVISELRELVREAMPDDEFHEMIDRDYSLSYDPSYENVTMRAWLTGLLGELEAGRGEA
                     MQSPTPLQTRGRN"
     gene            complement(1851..>1964)
                     /locus_tag="VGD55_02420"
     CDS             complement(1851..>1964)
                     /locus_tag="VGD55_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0869227.1"
                     /translation="ETLEDQAVTIRERDTMKQERVGLDAVVGYLSERLLDL"
BASE COUNT          339 a          637 c          641 g          347 t
ORIGIN      
        1 tccgatggct gtctcagccg cgggtcgcac cctcgagtcg atcgcgttgg acgtcgcgaa
       61 gatcgctggc cgattgcgcg cactcgctag cggtgacgat tcgtggacgg gtgcggccgc
      121 tgcttccgcg catgtccgca ccgcctcgtt gccaccgact ctcgacaaag cgaacgcgtc
      181 gtacgcagct gccggaagtg cgctgggctc gtacgcgcga acgctcgctg atgcacaaga
      241 ccagtcttcg tctgcaatcg ctgcggccga tcgcgcgagt gcggagctag cagctgcagt
      301 ctccgcacac gctgcggcgg cggcgcgcga tgcgtcggcc ttggctgccg cgcgcacggc
      361 tggactgcct ccgccggcct caaccgcgcc gcggtacgag gcgtcgatcg atgaggcatc
      421 ggcgcagctc aagagagcgg tcgcagcgaa cgacgatgcg cacgagcaac agcgacaggc
      481 tgcgcggatt gcagcatctg cgttgcagca ggcgtcgcgt gccggcatcc acaatcagtc
      541 gtggttccac cacgtcacgc actcggttgg tcattgggcg tcgacgcact ggacggcgac
      601 gctgcgcgaa gtctcgaagg tcggcacgat tgtttcggcg ttagctggag ccgccgcgct
      661 gattctcgcg gtcgctggcg tcgcctttcc gcccttggaa gcagcagccg ctgcgctcga
      721 aacggtctcg ctcgtcagcg cagtagctgc aggaatggcc gacaccgcgt tggctgcaac
      781 cggcaaggct tcgtggacgt cagtcggcgt cgacgccttg agcttcgcgc ccgccggcgt
      841 cggcaagctc gtcaccaaag ccgcaccact aatccgcgag gggcgcctgc tcaagccaac
      901 gacagtcgtc cacgccagca acggcgaggc cacccgcgca gtacaggcgt cccaaggatc
      961 gaggctcgcc tacccgggcg gcctgggagc gaacgacgcc ctcggtggcc acgtgttgat
     1021 ccacgttggc gcccgggacg aggacctcgc cgcacgcaga ctgccggccg catcaacttt
     1081 cactgatcga gcgaccgccg aacacgcatt ggcggaagta caatcgtcga gtgcaagcga
     1141 gattcgcacc tggttggcag tcgcgccgca aggcgagcgg gcgtcgtttg gcgtcacgtt
     1201 tgacacgcct gttgggcgga tactcgttcg tggccagcgt aaatccattg gctgcgttag
     1261 cgcagttgcg gtactccgtg cagaccgttc agctccattg ggctacatcg tcttgacagg
     1321 atttgttgca ccatgacgcg ccgctacttc gccgtccaag agatgttcgt cctgtacttc
     1381 cacgaggact ggcgccttga cgcagactct cggctcgagg tcgcgacgga cttcctagcg
     1441 acggctagtc ccgtcctgat cgacgacgtg atcagcgaac ttcgagaact cgttcgtgag
     1501 gccatgcccg atgacgagtt ccacgaaatg attgatcggg actattcgct gtcctacgac
     1561 ccgtcatacg agaacgtcac catgcgcgct tggctgacag gcttacttgg ggagctagaa
     1621 gctgggcgcg gcgaggcgat gcagtcgccg acgccacttc agacccgcgg acggaactag
     1681 aggtcgggtg ttgctggtga gcggtcgttc ttgcggccgt cgagcatcag cggtcccggc
     1741 aaatggctgg ggcgatcccg agctgagatc gagcgcactt actgccccag gagggcagca
     1801 aatgcgcaag attcggcgat ggccaggggg agaagccgcg gccgcgacac ctagaggtcg
     1861 agcaggcgct ccgacagata gccgaccacg gcgtccagcc ccactcgctc ttgcttcatc
     1921 gtgtcgcgct cgcgaattgt gacggcctga tcctcgagcg tctc
//
LOCUS       DASWVF010000139         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_233146, whole genome shotgun sequence.
ACCESSION   DASWVF010000139 DASWVF010000000
VERSION     DASWVF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233146"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VGD55_02425"
     CDS             <1..345
                     /locus_tag="VGD55_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0869228.1"
                     /translation="EADQRIVASWLDTGEFSAGYKLTHEGASGYAFYVLHDGAADVVV
                     GGNVVRTLGPGDFFGELSMLGDGRQTATVTVTAPSTIWTMFGSRFRELQQEHPAIAAA
                     IEKVAAERVTPQ"
     gene            complement(348..>2547)
                     /locus_tag="VGD55_02430"
     CDS             complement(348..>2547)
                     /locus_tag="VGD55_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0869229.1"
                     /translation="LVVIGWFLFIVVAQGISGALGGPSYKDTFSLPHTETASVAKVLK
                     SAGLADQSGATGTVVMKATTGTLTSPPADVSPALLKVCSSADQVAVIRTPWQTIDCTQ
                     NAGKGALGAGDPKLLNAANGSTTALVNITWNSNHYDQKLFTGVYNDLKTLRSGGLEVE
                     FTGNAFEGQGQNTQGLPPFAFGFFAALIILVFVFRTLGATVLPLASAVAALGAGLGLI
                     GMLTHVMNVSNITPELTELMVLGVGVDYALFIVTRHRRNLRRGVGVTDSIITSMNTSG
                     RAVLFAATTVCIALLGLLALGVSFFNGMAIGAAVAVTLTAAASLTLLPALLSLFGLKV
                     LPRKQRRAVRNGEFIGSEPTTGFWSRWSIIVAEHKLVLSVVAAAVIVVLALPFFSMRL
                     GAGDQGNDPKGTTTRTGYDLIAQGFGVGYNSTLEAVVSGPGATDPAYLTKVSDALQST
                     PGVDPTSVHAVPLNSTVAFVSFKSTTSPQDAKTYALVKDLRANVLPKVYSGTSDHIYV
                     FGDTAIQVDFAKVLAAKMPLFIAAVVGLSFLLLLIAFRSLVIPLTAALMNLLAAGASF
                     GVVVAIFQFGWGSEALGVGKGGPIDAWAPVMFFAILFGLSMDYQVFLVSRMHEEWVHT
                     KDNRRSIIVGQAETGSIITAAALIMIAVFGGFVLGDQRAIKLFGIGLASAVFLDAFLL
                     RTILVPSLMHLLGDRNWFLPRWLDRVTPKVSFEPPDELELEDESVLVGNG"
BASE COUNT          504 a          802 c          843 g          398 t
ORIGIN      
        1 gaagcggatc aacgcatcgt cgctagctgg ctagacaccg gcgagttctc cgctggctac
       61 aagctgacgc acgagggtgc ctcgggttac gcgttctacg tcctgcatga cggggcggcg
      121 gatgtcgtcg tcggcggcaa cgtggtgcgc accttggggc cgggcgactt cttcggcgaa
      181 ctttcgatgc ttggtgacgg tcggcagacc gcgacggtga cggtgacggc accatcgacg
      241 atctggacga tgttcggcag ccgcttccgg gaactccaac aggagcaccc ggcgatcgcc
      301 gccgctatcg agaaggtcgc ggcggagcgg gtaaccccgc agtagcacta gccgttgccg
      361 accaggaccg actcgtcttc gagctcgagt tcgtcgggcg gctcgaacga caccttcgga
      421 gtgacccgat cgagccaccg cggcaagaac cagttgcggt caccgagcaa gtgcatcagc
      481 gagggaacca ggatcgtgcg cagcaagaat gcatcgagga acacggcgct cgcgagcccg
      541 ataccgaaca gcttgatcgc ccgctggtcg ccgagcacga acccgccgaa caccgcgatc
      601 atgatgagag cagcagcggt gatgatgctg cccgtctcgg cctgaccgac gatgatcgag
      661 cgacggttgt ccttcgtgtg cacccattcc tcgtgcatgc gactgaccag gaacacctgg
      721 tagtccatag agagcccaaa gaggatggca aagaacatga cgggtgccca cgcatcgatg
      781 gggccaccct tgccgacgcc gagcgcctca gaaccccagc cgaactggaa gatcgccacg
      841 acgacgccga acgatgcccc cgcggcaagc aggttcatca gggccgcggt cagcgggatg
      901 acgaggctgc ggaatgcgat caggagcagc aggaaggaaa gcccgacgac ggccgcgatg
      961 aacagcggca tcttcgcggc gagcaccttc gcgaagtcga cctggatcgc agtgtcaccg
     1021 aacacgtaga tgtgatcgct agtgccgctg tagaccttcg gcaacacgtt ggcgcgcagg
     1081 tctttcacca gcgcgtaggt cttcgcgtcc tgcggcgatg tcgtcgactt gaacgacacg
     1141 aaggcaaccg tgctgttcag cggaacggcg tgcacgctgg tcgggtcgac acccggcgtc
     1201 gactggaggg cgtcactgac cttcgtcagg tacgcaggat cggtcgcccc tggcccacta
     1261 accacggctt caagcgtcga gttgtaaccc acgccgaaac cttgcgcaat caggtcgtac
     1321 ccggtgcgcg tcgtggtccc cttcggatcg ttgccttggt cgccggcgcc gagccgcatc
     1381 gagaagaacg gcagcgcgag caccacgatg acggccgccg ccacgacgct caagacgagc
     1441 ttgtgctcgg cgacgatgat cgaccagcgc gaccagaagc cggtagtggg ctcggagccg
     1501 atgaactcac cgttgcgcac cgcccgccgt tgcttgcgcg gcaggacctt cagaccgaac
     1561 agcgacagca aggccggcaa cagggtcagc gaggcggctg ccgtcagggt cactgcgaca
     1621 gccgcgccga tcgccatgcc gttgaagaag ctcacaccaa gagccagcag gccaagcaac
     1681 gcgatgcaga cggtcgtggc ggcgaacagc accgcgcgcc ctgaggtgtt catcgaggtg
     1741 atgatcgagt cggtcacccc gactccgcgc cgcaggttgc ggcggtgtcg tgtcacgatg
     1801 aacaacgcgt agtcgacgcc gaccccgagc accatcaact cggtcagctc aggggtgatg
     1861 ttcgacacgt tcatgacgtg agtgagcatg ccgatcagcc cgaggccggc accgagcgcg
     1921 gcgacggcac tggccaacgg caggaccgtc gcgccgagcg tgcggaacac gaagaccaag
     1981 atgatgagag cggcgaagaa gccgaacgcg aacggcggga ggccttgcgt gttctgcccc
     2041 tggccctcga acgcgttacc tgtgaactcg acctcgaggc cgccgctgcg caacgtcttg
     2101 agatcgttgt acacaccggt gaacagcttc tggtcgtagt ggttggagtt ccaggtgatg
     2161 ttgaccagcg cggtggtcga cccattagcg gcgttgagca gcttcggatc cccagcgccg
     2221 agcgcacctt tcccggcgtt ctgagtgcag tcgatggtct gccatggcgt gcggatgacg
     2281 gcgacctggt cggccgacga gcaaaccttc agcagcgcgg gggatacgtc ggccggtggt
     2341 gacgtgagcg tgcctgtggt ggccttcatg accacggtgc cggtcgcgcc gctctggtcg
     2401 gccaagcctg cgctcttcag aaccttcgcg accgaggccg tctcggtgtg cgggaggctg
     2461 aacgtgtctt tgtacgaagg gccaccgagg gcaccagaga tgccttgcgc gaccacgatg
     2521 aaaagaaacc agccgatgac caccagc
//
LOCUS       DASWVF010000140         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_233190, whole genome shotgun sequence.
ACCESSION   DASWVF010000140 DASWVF010000000
VERSION     DASWVF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233190"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VGD55_02435"
     CDS             <1..1533
                     /locus_tag="VGD55_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869230.1"
                     /translation="KLVRPTVLSLAICGLAIVSNASVADDVASFKGFDDHGQFKLEHG
                     SLVISSSTYDRSKGAVAALTIGTTLPDSATATTLAVASNNYVTVWNNATVDGSFGVTS
                     PIELSDVDASSGRPLHRLHVPEEAVVTSFSSKSELGLHIAEDWRGSRVVFVAYAGSGV
                     GALDVSNSDAVPGQDPTNPVTFAFGTNYAFPRTIVSMDEHGRFAYTPTVDYGGNNGRS
                     ALMAPNGLYYAVGNANNGNAATFGPSNGTAPDVTETTGLEVVHPLDELWPSRWVPAGN
                     SAEIDPLLQFTFTTKPDKPGKDDNYRGVTEYGGALYFTKGSGSNGVDTVYSVDSLPTL
                     ASAASAKINIVPGFPTDSAKATGGDFTPFAVFFANATTMYVTDEGSGNATDAASHAGL
                     QKWSLSDGVWTLDYVLTKGLIGTVDTNLNGYDGQYPNVTTVGLRNLTGVVHRDGTVTL
                     WASTSTSSASGDEGADPNKVVRITDRLAATTSTGSVVGESFTTIAGPTYGKVYRGVAY
                     AE"
BASE COUNT          316 a          515 c          511 g          318 t
ORIGIN      
        1 aaattggtta ggcccactgt gctcagtctt gcgatctgcg gactcgcgat tgtgtcgaac
       61 gcaagcgttg ccgacgacgt agcctccttc aagggatttg acgaccacgg gcaattcaag
      121 ctcgaacacg gctccttggt catcagcagc agcacttacg accgcagcaa gggcgcggtt
      181 gcggctctga caatcggtac cacgctgccg gattcggcca ccgccaccac gttggcggtg
      241 gccagcaaca actatgtcac cgtatggaat aatgcaacgg tcgacggcag cttcggcgta
      301 acctcgccga tcgagttgag cgatgtggac gcatcgagcg gccggcctct acacaggctg
      361 cacgtgcctg aggaagcggt cgtcaccagc ttctcatcga aatccgagct gggtctgcac
      421 attgccgagg actggcgcgg atcccgcgtg gtattcgtag cctatgccgg ctcgggcgtg
      481 ggtgcgctcg acgtatcgaa ctccgatgcc gtgccggggc aggatccgac caacccggtc
      541 acctttgcct tcggcacgaa ctatgcgttt cctcgcacca tcgtctcgat ggacgagcac
      601 ggccgtttcg cctatacgcc gaccgtcgac tatggcggta ataacggccg gtcggccttg
      661 atggccccca acggactcta ctacgccgtc ggtaatgcca ataacggcaa cgcggccacc
      721 ttcggcccaa gcaacggcac ggcccccgat gtcaccgaga cgaccgggct cgaagtggtc
      781 catcccctcg atgagctctg gccgagccgt tgggtcccgg ccggcaattc cgccgagatc
      841 gatccgctgc tgcaattcac gttcacgacc aagcccgaca agccgggcaa ggatgacaac
      901 taccgcggtg tcaccgaata tggcggtgcg ctctatttca ccaaaggcag cggcagcaac
      961 ggtgtcgaca cagtgtatag cgtcgattcg ctgccgacgc tcgcgagcgc ggcatcggcc
     1021 aagatcaata tcgtgccggg gtttccgacc gattccgcca aggcgaccgg cggcgacttt
     1081 actcccttcg cggtgttttt cgcgaacgcg actacgatgt atgtgacgga tgagggctcc
     1141 ggcaacgcaa ccgacgcggc aagccatgcg ggtctgcaga agtggagcct gagcgacggc
     1201 gtttggacgc tggactatgt gctcaccaag ggcctgatcg gcaccgtcga taccaatttg
     1261 aacggctacg atggccaata tcccaatgtg acgaccgtgg gtctgcgcaa tctgaccggt
     1321 gtcgtgcacc gcgacgggac ggtgacgctc tgggccagca cctcgacctc gagcgcttcg
     1381 ggtgatgagg gcgcggaccc gaacaaggtg gtgcggatca ccgaccgcct cgcggcgacc
     1441 acctcgacgg gatcagtcgt cggcgagtcg ttcacgacca tcgcgggacc gacgtacggt
     1501 aaggtctatc gcggggtcgc atacgcggag tagggctagc gtacgcggat tgggcctgtc
     1561 tattttctga ttactgtctt gtcgtcatgc tgcttttttg caaagactga cttctccggg
     1621 tctgtgtcgc ggcgcagcgt ggggataatc ccacggcgct
//
LOCUS       DASWVF010000141         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_131398, whole genome shotgun sequence.
ACCESSION   DASWVF010000141 DASWVF010000000
VERSION     DASWVF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131398"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGD55_02440"
     CDS             complement(<1..637)
                     /locus_tag="VGD55_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0869231.1"
                     /translation="MPTVLTYSQAYREGVAEEMAANEKIFIMGTDIVLRGGHFAQVFG
                     LGEQFGASRVRDVPISEAAMLAAGVGSAVCGMRPIVDLNFLDFSFGGMDEICNQAAKM
                     SYMFGIQVPILIRATNGIALGGAQHNNAIESWFAEMPGLNVVMPSNPGDIKALIKSSL
                     RMNDPVLFLMHKALSGLRGTVGGPDDLIPLGQANICRPGNSVTMVTYGVNVA"
     gene            complement(647..1666)
                     /locus_tag="VGD55_02445"
     CDS             complement(647..1666)
                     /locus_tag="VGD55_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY0869232.1"
                     /translation="MSTSDLAEGSSAAAMLAMAPPPEVLREWLGAMLTIRRFETECVR
                     LSFAGKIPGGIHSSEGQEGVAIGSIRALTRDDVVSGTHRSHHHALAKGLPLRVVMAEL
                     FGRDDGLSGGRGGSMHLVDRSLNFLGSNGIVGAGLGIAMGASLAMQMQQQPFVAVGYF
                     GDGGANNGRVWESINLAALWKLPLIAICENNLYAVETHISNAMAGESVAARASGFGLP
                     TVTVDGQDVVAVYQATREARERAIAGEGPTFIEAQTYRYEGHNVGDVQNYREKAEVAQ
                     WRQERDPIARLRERMIDDAMLDDAQYAALEAAAEHAVADAIAYADASPWPTGADALTT
                     YPTNV"
     gene            complement(1663..>1891)
                     /locus_tag="VGD55_02450"
     CDS             complement(1663..>1891)
                     /locus_tag="VGD55_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY0869233.1"
                     /translation="SFTGIIPLGQLCLLTIGRIAPRLVPTDNGFSTTQTLQATLNVDH
                     RSIDGDVAARALDVFAQALGSVQTWVNGGQE"
BASE COUNT          357 a          613 c          572 g          349 t
ORIGIN      
        1 tcgccacgtt gacgccgtag gtcaccatcg tgacgctgtt tccagggcga cagatgttcg
       61 cttgacccaa cgggatcaag tcgtcggggc cacccacagt cccacgcaac cccgacaacg
      121 ccttatgcat taagaagagc acggggtcgt tcatccgcaa tgacgacttg atcaatgcct
      181 tgatgtcgcc ggggttcgac ggcatcacca cgttgagacc cggcatctcg gcgaaccatg
      241 actcgatcgc gttgttgtgc tgtgcgccac ccaacgcgat gccgttcgtg gcccggatca
      301 ggatcggcac ctggatgccg aacatgtagc tcatcttcgc ggcctggttg cagatttcgt
      361 ccatgccgcc gaaggagaag tcgaggaagt tgaggtcgac gatcgggcgc attccgcaaa
      421 ccgccgaacc aactccggct gccagcatcg cggcctccga gatcggaacg tcgcgaaccc
      481 gtgaggcgcc gaattgttcg ccgagcccga agacctgtgc gaagtgaccc ccgcgcagca
      541 cgatgtcggt gcccatgatg aagatcttct cgttcgccgc catctcttcg gcgacgcctt
      601 cgcggtaagc ctggctgtag gtcagcacgg ttggcatcag atgccctcag acgttagtcg
      661 ggtaggtggt cagtgcatct gcgccggtcg gccacggtga cgcgtcggcg taggcgatcg
      721 cgtcggcaac agcgtgctca gcagcggcct cgagagcggc gtactgcgca tcgtcgagca
      781 tcgcgtcgtc gatcattcgc tctcggagcc gggcgatcgg gtcacgttct tggcgccact
      841 gcgcgacttc ggccttctcg cgataattct ggacgtcgcc cacgttgtga ccctcgtagc
      901 ggtaggtctg cgcttcgatg aaggtcggcc cttcgccagc gatcgcgcgt tcgcgagcct
      961 cacgggttgc ctggtagacc gcgacgacgt cttgcccatc gacagtcacc gtcggcagcc
     1021 cgaaccccga tgcgcgagcg gcgacggact cccccgccat cgcgttcgag atgtgcgtct
     1081 ccacggcata gaggttgttc tcgcagatcg cgatcagcgg cagcttccag agcgcggcga
     1141 ggttgatgct ttcccagacc ctgccgttgt tcgctccgcc atcgccgaag tagccgaccg
     1201 cgacgaacgg ctgctgttgc atctgcatag cgagcgaggc gcccatcgcg atgccgaggc
     1261 cggcaccgac gatcccgttc gatccaagga agttcagcga gcgatcgacg aggtgcatgc
     1321 tgcccccacg gccgcccgac agaccgtcgt cacgaccgaa cagctcggcc atcaccactc
     1381 gtagtggcag gccttttgcc aacgcgtgat gatggctgcg gtgggtgccg gagacgacat
     1441 cgtcacgcgt caatgcgcga atcgacccga ttgccacgcc ctcctggccc tccgacgagt
     1501 ggatcccgcc cgggatcttg ccggcgaatg acagccgcac gcattcggtc tcgaagcgtc
     1561 gaatcgtcag catcgcgcca agccattcgc gcaaaacctc aggcggtggc gccattgcca
     1621 gcattgcagc agcggaggag ccttcggcaa gatcggacgt actcattcct gaccaccatt
     1681 cacccacgtc tggacagacc cgagagcctg tgcgaagaca tccagagcac gtgccgctac
     1741 atcaccatcg atggatcggt gatcaacgtt caacgtggct tgcaaggttt gcgtcgttga
     1801 gaatccgttg tcggtgggaa ccaaccgcgg agcgatacga ccgatggtca gtaagcacaa
     1861 ctgcccaagc ggaatgatgc ctgtaaacga a
//
LOCUS       DASWVF010000142         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_437190, whole genome shotgun sequence.
ACCESSION   DASWVF010000142 DASWVF010000000
VERSION     DASWVF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437190"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VGD55_02455"
     CDS             <1..962
                     /locus_tag="VGD55_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869234.1"
                     /translation="CERQAVAESGPERGTILVVDDDTDIVRFVEMNLRLEGYRVLIAR
                     DGLEALEMVAAEVPDLVLLDVMLPGVDGVEVTRRLRADSRTSTLPVIMLTAKTMTADR
                     VLGLTTGADDYIIKPFDTLELVARVKSTLRRNAEARAVSPLASLPGNVRIEDEIAGRV
                     SSGSPFAVAYLDLDNFKAFNDAHGFLRGDQVILLLATALRRAVVGANPPAFIGHVGGD
                     DFVLVCRPEQVEDLSFAAVEYFDDKSPALHDPADVARGYLEVVDRKGVLRDYPLVSVS
                     VGIATSERRRFDDYREVVIVATEMKGVAKGEIGSAIAVDRRAV"
     regulatory      1013..1158
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1239..>1681
                     /gene="ribD"
                     /locus_tag="VGD55_02460"
     CDS             1239..>1681
                     /gene="ribD"
                     /locus_tag="VGD55_02460"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0869235.1"
                     /translation="MVAAAGEADLVAATAMRRAIALAELGLGSTSPNPIVGCVIVGAD
                     GSVVGEGYHARAGEPHAEVIALRDAGGRAFGATAFVTLEPCRHTGRTPPCTQALIEAG
                     VSRVVFAVADPDPVAGHGADELRAAGVVVEEGLLRDEAARSNAAWL"
BASE COUNT          266 a          528 c          583 g          304 t
ORIGIN      
        1 gatgcgagag gcaggcagtg gccgagtcgg gtcctgaacg gggcacgatt cttgtcgtcg
       61 acgacgacac ggacatcgtc cgcttcgtcg agatgaacct gcgtctcgag ggctatcgcg
      121 tcctcatcgc tcgtgacggt ctcgaagcgc tggagatggt cgccgccgag gtacccgacc
      181 tggtgctgct cgacgtgatg ctcccgggcg ttgacggggt agaggtgacc cgtcgattgc
      241 gtgcggacag tcgcacgtcg acgttgccgg tcatcatgtt gaccgcgaag acgatgaccg
      301 ctgatcgggt gctcggactc accactgggg ccgatgacta catcatcaag ccgttcgaca
      361 ccctcgaact cgttgcccgg gtgaaatcga cgttgcggcg aaatgcagag gcgcgtgcag
      421 tctcaccgtt agcgagcctg ccgggaaacg tcaggatcga agatgagatc gccggccgtg
      481 tcagcagtgg cagcccgttc gccgttgcct atctcgatct cgacaatttc aaggcgttca
      541 acgacgcaca cggctttctt cgcggtgacc aagtgatcct gctgctcgcg accgcgttgc
      601 gtcgtgcggt tgtaggcgcc aacccgccgg cattcatcgg ccacgtcggg ggagacgact
      661 tcgtactcgt ctgccgtccc gagcaggttg aagatctgag cttcgccgcc gtcgagtact
      721 tcgacgacaa gtcgccggca ttgcacgacc ccgccgacgt cgcacgcggc tacctcgagg
      781 tggtcgaccg aaaaggagtc ctgcgcgact atccgctcgt ttcagtctcc gtcggcatcg
      841 ccaccagcga gcgtcgccga ttcgatgact accgcgaggt cgtcatcgtc gccactgaaa
      901 tgaagggcgt cgcaaagggc gagatcggct cggcgatcgc cgtcgaccgt cgcgccgtct
      961 gacctggacg acgggcgggg gctcggcgtg tcaaactggt gtcgtagaag caacgtgcgc
     1021 tccggggtcg gtgtaattcc gaaccggcgg tgatagtccg cgacccgatc gagaacctgc
     1081 gcagtttcag gtgaccggtc ggttgacccg gtggaactcc gggaccgacg gtgaaagtcc
     1141 ggatgggagg cggcgcgcgg gcggttcgcg cttgcatgcg cggcctcgtt cgccgacgtc
     1201 ttcgctcgcc ccggtgtcca acgggtgagg agacgcaggt ggtcgcagcg gcaggcgagg
     1261 cagatctcgt ggcggctaca gccatgcgcc gggcgatcgc ccttgccgaa ctcggtctcg
     1321 ggtcgacgag tcccaacccg atcgtcggct gcgtcatcgt cggagctgac ggctcggttg
     1381 tcggcgaggg ctatcacgct cgcgcgggtg agcctcatgc cgaagtcatt gccttgcggg
     1441 acgccggggg gcgggcgttt ggtgcgacag ctttcgtcac gttggagccc tgccggcaca
     1501 ccggccgaac gccgccgtgc acgcaggccc tcatcgaagc cggggtgtcg cgggtggtgt
     1561 tcgccgttgc cgaccccgac ccggtggccg ggcacggcgc cgacgagctg cgcgccgctg
     1621 gtgtcgtcgt cgaggaaggc ctgctccgcg acgaagcggc ccgttcgaat gcggcctggc
     1681 t
//
LOCUS       DASWVF010000143         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_335088, whole genome shotgun sequence.
ACCESSION   DASWVF010000143 DASWVF010000000
VERSION     DASWVF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335088"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGD55_02465"
     CDS             complement(<1..267)
                     /locus_tag="VGD55_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869236.1"
                     /translation="MSTTDGSILARAVLTSAVTCAAGGNEEVADDEAPADGDAGVDEP
                     LVETVEPAAAFELLLEWSSTRCTTVPLAAAETTTAATVSAAAWRM"
     gene            260..1177
                     /locus_tag="VGD55_02470"
     CDS             260..1177
                     /locus_tag="VGD55_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0869237.1"
                     /translation="MLINDTITSGASGRGGFGGLGGFQESGAGTGIIISSDGEIVTNA
                     HVVNGASSITVTLPNNGGTHTASIVGIDTTADLAVIKISGVSNLKPATFANSDTAEVG
                     DSVLAVGNALGYGGAPTVTEGILSAKGRALTGTDDNLSNLLQTDAAINPGNSGGPLVD
                     TTGQVIGIDVAVASGTTTEPAQNIGFAIPSNTVVSALPALKAGKGASSQSQGQPTQSG
                     TFLGVSVADATGGALVEAVQPGSPAATAGIQAGDLITSINGKAIADGNALQQAIRAEK
                     AGTTVTVGITRNGSPTTLKATLGTTQVSS"
     gene            1287..1961
                     /locus_tag="VGD55_02475"
     CDS             1287..1961
                     /locus_tag="VGD55_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0869238.1"
                     /translation="MSDVVTVAVTSSDGMAADELDALIQRLPEHEQRRATAMRLDSRR
                     RNFVLGRTLLRAAVARVAGGRPDAVVVEIEPSGRPVLARPLDSFSVSIAHSGGYVVVG
                     VANRPIGVDVEQLRHSVRFPRVAARVCSPDELRALDAMTDAARERAFLTVWSRKEAYG
                     KAIGRGLSFPMRSVTVGPHGSRILGGEGDWQVRDLELDPAYAAAVVAQRDGWQPHLDR
                     VERRLL"
     gene            2036..>4308
                     /locus_tag="VGD55_02480"
     CDS             2036..>4308
                     /locus_tag="VGD55_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869239.1"
                     /translation="MAQRRVRASKRHLVSAIAVLALGAPLAAVSTGATTATGATGLAV
                     SRVSGADRFATAAAIADAGWPGTLPANSTLLLATGATFPDAVAGSAAAGHLGVPLLLT
                     ASGSLSSSAAAEIDRLKPSQVALLGGTAALSAAVAQQVATHGATVVRWQGSDRYATAA
                     AISQATYPSGATNVYLATGTNFPDALAGAALAAVAGGPLLLTDPKTLPSETAAEIARL
                     HPSAIVVLGGTSAVSDGVANAAVSAAGGAALSRIQGPDRYQTADAVASVLVSVKGGTA
                     ASNGVLLATGLDFPDALAGAAWAGQSDRPLLLVPSTYVTPETWQTIQSLAAPSAVVLG
                     GTSAIAAGVESGLSSGNPPTSPPAPVVGGPTDWPTYHHDAQRTGLAAATPAFTGFSAG
                     WKASLDGAVYGQPIVLGTTVVAATEGGSLYGLSLSTGAVLWRAHIADPIAQSLLPCGD
                     IDPLGITGTPAYDPSDGLLLAVAETAGGHHMLAGVSLATGTIAFKRTLDPLSGTAIAS
                     QQRGAILIANGRVYVAYGGLAGDCASYIGQVVSVASDGSGTPIGWAVPTTREGGIWAT
                     AGPVADLDGTILVAAGNGASDTTYDGSDSVTRLSPTLARLDYFAPITWANDNDNDLDL
                     GSMSPVLVGGKVFIAGKRGNGYLLDPTHFGGIGGQVTQFGDGFCKPFGGASVSGITTY
                     LPCNDGIRAVSVTGSSMTIVWHAASNLTGPPVVASGTVYTTDGSGSIVALNATTGATV
                     GQISVGSLPHFASPSLSG"
BASE COUNT          698 a         1458 c         1428 g          724 t
ORIGIN      
        1 cattcgccag gcagctgcgc tcaccgtcgc tgccgtcgtc gtttcggcag ccgcgagcgg
       61 aactgtcgtg cacctcgtcg acgaccactc gagcaaaagc tcgaacgcag ctgcgggctc
      121 gacagtctcg accaacggct catcgacgcc cgcatcacca tctgccgggg cctcgtcgtc
      181 ggcgacctcc tcgttgccgc ccgccgcaca agtaaccgcc gacgttaaga cagccctcgc
      241 aaggatcgag ccgtccgtcg tgctcatcaa cgacaccatc acatcgggcg cgagcggccg
      301 cggcggcttc ggtggactcg gtggtttcca ggagagcggt gccggtacag gcatcatcat
      361 cagctccgac ggcgagatcg tgaccaacgc ccacgtcgtg aacggcgcga gctcgatcac
      421 cgtcacgctg ccgaataacg gcggcaccca cacggcgtcc atcgtgggca tcgataccac
      481 cgcagacctt gcggtcatca agatctcggg tgtttccaac ctcaagcccg cgacgttcgc
      541 caactccgac accgccgaag ttggcgacag cgtgctggcg gtcggcaacg cgctgggcta
      601 cggcggtgcc ccgaccgtga ccgagggcat cttgtcggcc aagggccgtg cactgaccgg
      661 gaccgatgac aacctgtcga accttctcca gaccgacgcg gcaatcaacc ccggcaacag
      721 tggcggaccg ctcgtcgaca cgaccgggca ggtcatcggc atcgacgtcg ccgttgcctc
      781 tggcacgaca acggagccgg ctcaaaacat cggtttcgcg atcccgagca acacagtcgt
      841 cagcgcgctt cccgcgttga aggcaggcaa aggtgccagt agccagagcc aagggcaacc
      901 gacgcagagc ggcacgttcc taggggtttc tgtcgccgat gcgacgggtg gtgcgctggt
      961 cgaggcagtt cagccgggca gccccgcagc cacggccggg atccaggccg gcgacctcat
     1021 cacgtcgatc aacggcaagg cgattgctga tggcaacgcg ctccagcaag cgatccgggc
     1081 agagaaggcc ggcacgacgg tgacggtagg aatcacgcgc aacggctcgc cgacgaccct
     1141 caaggcgacc ctcggtacga cgcaggtcag ttcgtagcac tgacctgcgc ggacggcggc
     1201 actgtctgtt aggacggtgc cgccttccgt ttgtgcgccg ctttttgcga gaagatggcg
     1261 cacgtgcacg cacctggagt cttcgattga gtgacgtcgt caccgtcgcg gtgacgtcct
     1321 ccgacggcat ggcggccgac gaactcgatg cgctgatcca gcgattgccc gagcacgagc
     1381 aacgtcgcgc cactgcgatg cgactcgatt cgcggcgacg caacttcgtg ctcggccgca
     1441 ccctgctgcg ggcggccgtt gcacgggttg cggggggaag acccgatgcc gtcgttgtcg
     1501 aaatcgagcc gagtgggcga cccgtccttg ctcgaccgct cgacagcttc tccgtgagca
     1561 tcgcgcacag cggcggctac gtcgttgttg gtgttgcgaa ccgccctatc ggggtggacg
     1621 tcgaacagtt gcggcattcg gtacggtttc cgcgtgtggc tgctcgggtg tgctcacccg
     1681 acgagctgcg cgccctcgac gccatgaccg acgcggctcg cgaaagggca tttctcaccg
     1741 tgtggtcgcg caaggaggcc tacgggaaag caattggtcg gggcctttct tttcccatgc
     1801 ggtcagtgac cgtcggtccg cacgggtcgc ggattctcgg gggcgaaggg gattggcagg
     1861 tcagggatct ggagctcgat cccgcgtacg cggccgctgt cgttgcacaa cgcgatggct
     1921 ggcaaccgca cctcgatcga gtcgagcgac gcctcctata acgcacccga ggttgacccg
     1981 gacgacctcg cggtgtctag ggtggtgcgc ggtgtgaacc gaaagggagg ccttcgtggc
     2041 gcaacgtcgc gttcgggcaa gcaagcggca cctggtgagc gcgatcgccg tgctcgcgtt
     2101 gggtgcacca ctggccgccg tctccaccgg cgcgacgact gctaccggcg ccacaggtct
     2161 agctgtcagc cgggtctccg gcgccgaccg gttcgccacg gctgccgcga tcgccgacgc
     2221 cgggtggccg ggcacgctgc ccgcgaacag cacgttgctg ctggcgaccg gcgctacctt
     2281 ccccgatgcg gtggccggtt ctgcagcggc cggacacctc ggcgtcccgc tgctattgac
     2341 tgcgagtggg tcgctgtcgt cttcagccgc tgccgagatc gaccggctca agccttccca
     2401 ggttgcgttg ctcggaggca ccgcggcact aagcgccgca gtcgcgcagc aggttgcgac
     2461 ccatggcgcg accgtcgtgc gctggcaggg cagcgatcgg tacgccaccg cggcggcgat
     2521 ctcgcaagcg acgtatccaa gcggcgcgac caatgtgtat ctcgccactg gcacgaactt
     2581 ccccgacgcc ctcgcgggtg cagcgctggc tgcagtcgca ggtggcccgt tgctgctgac
     2641 tgacccgaaa acgttgccga gcgagacagc ggccgagatc gctaggttgc atccgtcggc
     2701 gatcgtcgtg ctcggcggga cctcggcggt gagcgatggg gtcgccaacg ccgcggtttc
     2761 ggctgcgggt ggtgcggctc tgtcgcgcat ccaagggccc gaccggtacc agacggccga
     2821 tgctgtcgca tcggtgctcg tgtcggtcaa gggcggcaca gcagcgagca acggcgtgtt
     2881 actagccacc ggcctcgact tccctgacgc actcgcgggt gccgcgtggg ccggccaaag
     2941 tgataggccg ttgctgctcg tcccatcgac ctacgtcacg ccagaaacct ggcagacaat
     3001 ccaaagtctt gcggcaccaa gcgcggttgt gctcggtggc acgagtgcga tcgccgcggg
     3061 agtggagtct ggccttagtt cgggcaaccc acctacctcg ccaccggcgc cggtcgtcgg
     3121 cgggccgacc gactggccga cctaccacca cgacgcgcaa cgcaccgggc tcgctgcggc
     3181 gacgccggct ttcaccggtt tctctgcggg ttggaaagcg tcgttggacg gcgcggtgta
     3241 cggccaaccg atcgtgctcg ggacgacagt cgtcgccgct accgaaggcg gctcgctcta
     3301 cgggctgtcg ttgtcgaccg gagccgtgtt gtggcgggcg cacatcgctg acccgatcgc
     3361 gcaatcgctc cttccgtgcg gagacatcga tccgctcggc attaccggca ctcccgccta
     3421 cgacccgagc gacggacttc tgcttgccgt tgccgaaacg gctggtggcc accacatgct
     3481 ggccggcgtc agcctcgcta cgggcacgat tgccttcaag cgcaccctcg atccgttgtc
     3541 aggcaccgcg atagcgagcc agcagcgcgg cgcgatcctg atcgccaacg gccgggtcta
     3601 cgtcgcgtac ggcggtcttg ccggggactg cgccagctat atcggccagg tcgtctcggt
     3661 cgcaagcgat ggcagcggga cgccgattgg ctgggccgtg ccgaccactc gtgagggcgg
     3721 tatctgggcg accgccggac cagtcgcgga cctcgacggc acgatcctgg tcgccgccgg
     3781 caacggtgcg tcggacacga cctacgacgg gtctgattcg gtgacgcgcc tctcgccaac
     3841 ccttgctcgc cttgattact tcgccccgat cacttgggca aacgacaacg acaatgacct
     3901 cgatctgggc tcgatgtcgc cagtgctcgt cggcggcaag gtgttcatcg cgggcaagcg
     3961 cggtaacggc tacctgctcg atccgaccca cttcggcgga atcggcggcc aggtgacgca
     4021 gttcggcgat ggtttctgca agcccttcgg cggggcatca gtgtcgggca tcacgaccta
     4081 cttgccgtgc aacgacggca ttcgggcggt ctccgttacc ggcagcagta tgacgatcgt
     4141 ctggcacgca gcgtcgaacc tgaccggacc tccggtcgtc gcgtcgggca cggtgtacac
     4201 gaccgacgga tccgggtcga tcgtcgcact caacgccacg acgggcgcta cggtcggcca
     4261 gatctcggtc ggttcgctgc cgcatttcgc gtcacccagc ctgtcggg
//
LOCUS       DASWVF010000144         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_233352, whole genome shotgun sequence.
ACCESSION   DASWVF010000144 DASWVF010000000
VERSION     DASWVF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233352"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VGD55_02485"
     CDS             complement(<1..469)
                     /locus_tag="VGD55_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869240.1"
                     /translation="MNMPGPARGTILVVDDDADIVRFVEMNLRLEGYRVLVARDGVEA
                     LEMVTAEVPDLVLLDVMMPGFDGVEVTRRLRADSRTSTLPIIMLTAKTLSADRVLGLT
                     TGADDYIIKPFDTLELVARVRSTLRRNAEARAVSPLTSLPGNVRIEDEIAGRVL"
     gene            complement(536..1201)
                     /gene="rpe"
                     /locus_tag="VGD55_02490"
     CDS             complement(536..1201)
                     /gene="rpe"
                     /locus_tag="VGD55_02490"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0869241.1"
                     /translation="MSVQIAPSILSADFARLADEAAAVAGVADWLHVDVMDNHFVPNL
                     TLGAPVVAALHAATALPLDCHLMIDDPDRWAASYAEVGSANVTFHAEAATDVVQTARS
                     IRAAGARAGLALKPASDVEDYLDVLGEFDMLLVMTVEPGFGGQAFLATMMPKVASARK
                     FLRDSGLPLWIQVDGGVTADTIEECARAGADVFVAGSAVYGAADPAAAVTALRRRAQA
                     ALE"
     gene            complement(1263..>2357)
                     /locus_tag="VGD55_02495"
     CDS             complement(1263..>2357)
                     /locus_tag="VGD55_02495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY0869242.1"
                     /translation="VLRLGTHQLLATRIGDHAAVDESVKLVREKVGHGPTGLVNAVLR
                     KVAVRDLAGWCATVAPRFDIDPIGNLALTYSHPRWIVEAFRDALDGDLDETSALLIAD
                     NVRPAVTLVARPGRSDVDELVSLGARPGQWAPTAAILDGGAPANFAPVRDGRAGVQDE
                     GSQLVALALAAAPIEGRDLRWLDMCAGPGGKAALLAGLADARGARLLAVERQPHRARL
                     VAHAVGITADVAVADGTTPPWAPASFDRVLVDVPCSGLGALRRRPEARWRRTPDDIEA
                     LRPQQLALLRSAISAVRPGGLVGYVTCSPHRGETGALVDDAVGPSIERVDVRPYLPGV
                     ALLGQGPDVQLWPHRHGTDAMYISLLRKPA"
BASE COUNT          389 a          818 c          801 g          349 t
ORIGIN      
        1 tgagcacccg gccggcgatc tcgtcctcga tgcgcacgtt gccaggcagg ctcgtcagcg
       61 gcgacaccgc gcgggcttcg gcgttacgac gcaaggtcga acgcacgcgg gcgaccagct
      121 cgagggtgtc gaacggcttg atgatgtagt cgtcggcgcc tgtggtgagg ccgagcacgc
      181 ggtcggcgct cagcgtcttg gccgtgagca tgatgatggg tagcgtcgac gtccggctgt
      241 cggcgcgcag ccgccgggtg acctcgacgc cgtcaaagcc cggcatcatg acgtcgagca
      301 gcactaggtc gggcacttcg gccgtcacca tctcgagcgc ctcgacgcca tcgcgggcga
      361 cgagcacccg gtaaccctcg aggcgcagat tcatctcgac gaaccggacg atgtcggcgt
      421 catcgtcgac aacaagaatc gtgccccgtg cggggccagg catattcatc ccccgtgcct
      481 ttccagctgc agggttttga tctgcatccc tttcaatctc ggcatgggca gtggattact
      541 cgagggcggc ctgcgcccgg cggcgcaaag ctgtgaccgc cgcggccgga tcggcagcac
      601 cgtagaccgc agagccggca acgaacacgt cggctccggc ccgagcgcac tcctcgatgg
      661 tgtcggccgt gacgccgccg tcgacctgaa tccacagcgg cagcccgctg tcgcgcagaa
      721 acttccgcgc gctggcgacc ttcggcatca tcgtcgcaag aaatgcctgg ccgccgaagc
      781 ccggctcgac ggtcatgacc agcaacatgt cgaactcacc cagcacgtcg aggtagtcct
      841 cgacgtcgga cgcgggtttt aacgcaaggc cggcgcgcgc accagcagcc cgaatcgatc
      901 gtgcagtctg gacgacgtcg gtggccgcct cagcgtggaa cgtgacgttg gcggagccga
      961 cttcggcgta actcgccgcc caacgatcgg gatcgtcgat catcaagtgg cagtcgagcg
     1021 gcaacgcagt cgcggcatgc aaagcagcaa ccacgggagc gcccagagtg aggttcggca
     1081 cgaagtggtt gtccatgacg tcgacgtgca accagtcggc gacccccgcg acagccgctg
     1141 cttcgtcggc caacctggcg aaatccgcgg acaggatgct cggcgcgatc tgcaccgaca
     1201 tgcgcgccgc cctccaggta gtgcgaccga attgtccgcg gcccgatcct atgacgctgc
     1261 gctcacgcgg gcttgcgcag cagcgagatg tacatcgcgt cggtgccgtg ccggtgcggc
     1321 cacagctgca catcggggcc ttggcccagc aatgcgacgc cgggcaggta cggccggacg
     1381 tcgacccgct cgatgctggg cccgaccgcg tcgtcgacga gtgccccggt ttcaccgcga
     1441 tgaggcgaac acgtgacgta cccgacgagc cctcctggcc gcaccgcact gatggccgat
     1501 cgcagcagtg ccagttgctg cggacgcagg gcctcgatgt cgtcgggcgt gcgtcgccag
     1561 cgggcttctg gccttcgccg cagtgcgcca aggcctgaac acgggacgtc gaccagaacc
     1621 cgatcgaacg aggccggcgc ccacggtggc gtcgtgccgt cggcgaccgc gacatcggcg
     1681 gtgatgccca ccgcgtgggc gacgagcctg gcccggtgag gctggcgttc gaccgccagc
     1741 agccgggcgc cgcgcgcgtc ggcaagcccg gcgagtagtg ccgccttgcc gccagggccg
     1801 gcgcacatgt cgagccatcg caggtcgcgg ccctcgatcg gtgcggccgc caaggccagt
     1861 gcgaccagct ggctgccttc gtcctgaacc cccgcacggc cgtcgcgcac gggcgcgaag
     1921 ttcgccggag caccgccgtc gaggatcgcg gcagtcggtg cccactgtcc cggccgcgct
     1981 ccgagactca ccagctcgtc gacgtccgac ctcccgggac gagcaaccaa cgtgaccgcg
     2041 ggtcgcacgt tgtcggcgat caacagggcg gatgtctcgt cgaggtcgcc gtcgagcgca
     2101 tcgcggaacg cttcgacgat ccagcgcggg tggctgtacg tcagggcgag gttgccgatc
     2161 ggatcgatgt cgaaccgcgg agcgactgtt gcgcaccagc ccgccaggtc gcgaacggcg
     2221 accttgcgaa gcaccgcatt cacgagcccg gtcggcccgt gaccgacctt ctcgcgcacc
     2281 aacttgacgg actcgtcgac cgcggcgtga tcgccgatgc gggtcgccag cagctggtgg
     2341 gttccgagtc gaaggac
//
LOCUS       DASWVF010000145         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_411712, whole genome shotgun sequence.
ACCESSION   DASWVF010000145 DASWVF010000000
VERSION     DASWVF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411712"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1356)
                     /locus_tag="VGD55_02500"
     CDS             complement(130..1356)
                     /locus_tag="VGD55_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0869243.1"
                     /translation="MRILAVLPHFDPDVAPTGVIATRLVEELGMLGHHIDVVTSLPWY
                     EHHDVLPEWRGKWVRREQTSWGRITRVHPFPTGDKRSIPKRAVGFAAFCALDAAVAAV
                     GGRVDVTFAMTPPLPMAATGWLATLARRAPLVLNVQDVFPDVAVELGLLRGRTLISAA
                     SALERWSYARCAAVTVLSADMRANLAAKMRRPQRLRVIPNFVDVEEVQPGPNENSYRA
                     EYGLTGKTVVMYAGNVGMSQSLDLLVHAARSTRDRDDIVYVINGGGSTLPDLEEQAGD
                     LSNLRFVPMQPIQRLPELLAAADLHVVPLKAGLARSSVPSKTYSIMAAGRPLVASVDE
                     GSEVQRVVEAAGAGLAVSPDDAEAFTAAVLKLADDAGVRETMGAAARAYVERTASPRG
                     VAQAYAAVFEDVTSHK"
     gene            complement(1353..>1690)
                     /locus_tag="VGD55_02505"
     CDS             complement(1353..>1690)
                     /locus_tag="VGD55_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869244.1"
                     /translation="TSEQLAALYAGASLLAFPSRFEGFGIPVVEAMTAGCPVVAADAT
                     ALSALVGTAGMLVDPDDAEAWAGVMSDLLADDAARQRLSEAGRARAAILAAVDPVQLL
                     VDVYARVGA"
BASE COUNT          293 a          583 c          547 g          267 t
ORIGIN      
        1 gacgaggcgc taggccacgc cgacctgacg ttgcgcaact gcccgcgcat cgacatgcca
       61 gcgccgccgc gcgccttccg cgcccgctag ctcagtaatc gagcgccgac cagccagcgg
      121 gcctggcgct tacttgtggc tagtcacgtc ttcgaacacg gctgcgtacg cctgtgcaac
      181 gccgcgcggc gacgccgtcc gctcaacgta ggcacgtgcg gccgcgccca tggtttctct
      241 gacccctgcg tcgtcggcca gcttcagcac tgccgccgta aacgcttcgg cgtcgtcagg
      301 tgacaccgcg agacccgcgc cagcagcctc gacgactcgc tgcacttcgc tgccctcgtc
      361 gacgctggcc accagaggtc gacccgcggc catgatcgag tacgtcttcg acggcacact
      421 cgaccgtgcc aatccagcct tcaatgggac gacgtggaga tcggccgcgg cgagcagttc
      481 aggtagtcgc tggatcggct gcatcggcac gaaccgcagg ttcgacaggt cgcccgcctg
      541 ctcctcgaga tcgggcagcg tcgacccgcc gccgttgatg acgtagacga tgtcgtcgcg
      601 gtcacgagtc gatcgcgccg cgtgcacgag aaggtcgagc gactgtgaca taccgacgtt
      661 gccggcgtac atcaccacgg tcttgccggt taggccgtac tccgcgcggt aggagttttc
      721 gttcggccct ggctgtactt cctcgacgtc cacgaagttc gggatgacac gcagtcgttg
      781 cggtcggcgc atcttcgccg cgaggttggc tcgcatgtcg gccgacaaca cggtgactgc
      841 ggcgcatcgc gcgtaagacc accgctcgag cgcgctcgcc gccgagataa gcgtgcggcc
      901 gcgaagcaac ccgagctcga ccgccacgtc gggaaagaca tcttgcacgt taagcacgag
      961 cggcgcacgg cgcgccaatg tcgccaacca ccccgttgca gccatcggca gtggcggggt
     1021 catcgcgaac gtgacatcga ctcgcccacc gacggcggcc actgcggcgt cgagtgcgca
     1081 gaatgcggcg aacccgactg ctcgcttcgg gatgctgcgc ttgtcaccgg tcgggaacgg
     1141 atgcacgcgc gtgatgcgac cccacgatgt ctgctcgcgg cgcacccatt tgccgcgcca
     1201 ctcaggcagc acatcgtggt gctcgtacca cggcagtgag gtgacgacat cgatgtgatg
     1261 gccgagcatt ccgagctctt cgaccaagcg agtcgcgatg actcccgttg gcgcgacgtc
     1321 gggatcgaag tgcgggagca ctgccaggat cctcatgcgc cgactcttgc gtagacgtcg
     1381 acgagcagct gcacggggtc gaccgcggcc agtatggcgg cgcgtgctcg accggcttcg
     1441 gacagccgtt ggcgtgcggc gtcatcggcg agcaggtcgg acatcacacc agcccaggct
     1501 tcagcgtcat cggggtcgac cagcatgccg gcggtaccga ccagcgctga caacgcggtc
     1561 gcgtcggccg ccaccactgg acacccagca gtcatcgcct cgacgaccgg aatgccgaaa
     1621 ccctcgaaac gggagggaaa tgcgagtagc gaggcacccg cgtataacgc ggcgagctgc
     1681 tcggaggtca
//
LOCUS       DASWVF010000146         7397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_437202, whole genome shotgun sequence.
ACCESSION   DASWVF010000146 DASWVF010000000
VERSION     DASWVF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437202"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VGD55_02510"
     CDS             complement(<1..378)
                     /locus_tag="VGD55_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0869245.1"
                     /translation="MSAGVVTGEAVLLDVRLAHWPSRLLAKLVDLVAQFILLFILLLV
                     LGRLFDGADDDTAAALSVCIIVFVFVGYNVILETVWRGRTLGKAWLGLRVVRDDGGPA
                     RFRHALVRGIMFPLEGWGPALIAS"
     gene            399..1409
                     /locus_tag="VGD55_02515"
     CDS             399..1409
                     /locus_tag="VGD55_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY0869246.1"
                     /translation="MDVDVFVAAHQASWARLEALVERADRPQRLTGAEVDELIDLYQR
                     TATHLSVIQSRSPDAHLISRLSVLTARARAAITGSHDPAWSDFSRFFVVTFPAVLYRT
                     ARWWVSTALASLALMTAIGVWTATHRGVVDSLVSPADVRQLVSHDFKDYYSAHPATDF
                     AANVWTHNAFLTGATLALGIFLGIPTIYLLWQNCVNVGVIGGIMSANGKLPEFFGLIL
                     PHGLLELTAVFIASGTGLRIGWTLIEPGARRRADALAQEGRASFAIALGLVAMLMVSG
                     AIEAFVTPSPLPTWARVSIGVIVELGFLTYVFVLGSRAVRAGDIGDLDEALNADVVEA
                     TG"
     gene            complement(1410..2720)
                     /locus_tag="VGD55_02520"
     CDS             complement(1410..2720)
                     /locus_tag="VGD55_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0869247.1"
                     /translation="MALTRRAAIVALVGVLLVFAAPVRGFTVVIVEGALVVAICVDLA
                     LAGSVRALEVSRYGDTARRLDEVSEVGVQVVNRGGRRVRGVLRDAWPPSASASPTRRA
                     VDVPAGERRRWVTTIQPGRRTELQAGRVTVRSVGPLGLAARQGNHEVPWSLRVLPAFP
                     SRRYLPEKLAKLRILDGRTAARVRGQGTEFDSLRDYVEGDDARSIDWRATARRSAVVV
                     RTWRPERDRRIVLVLDTGRTSAARIGAAPRLDAAIDAALLLGTLASRAGDKVDLIAVD
                     DRPRMLTRGSASGSEGHGDALAGLLESMAGLHPRLVETDGRRLVAEVLRRVRQRALVV
                     LFTTLDEAAITEGLLPVLPSLTRKHVVMLASVADPQVLAMLDRSDDARDVYDAAAAER
                     TLAAHRRVATLLRRLDVEVVDAGPAEFASRVSDAYLAMKAAGRL"
     gene            complement(2728..3696)
                     /locus_tag="VGD55_02525"
     CDS             complement(2728..3696)
                     /locus_tag="VGD55_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0869248.1"
                     /translation="MTSTSSIEQSREAFAAVRAEVAKAVVGQDPVVSGLVVALLADGH
                     VLLEGVPGVAKTLLVRSLAAALSLETKRVQFTPDLMPGDVTGSLIYDSRSAEFEFRQG
                     PVFTNLLLADEINRTPPKTQAALLEAMEERQVSVDGTPRPLPEPFMVIATQNPVEYEG
                     TYPLPEAQLDRFLMKLNVPLPGRDDEFGMLTRHANGFDPRDLAGAGLLAVAGPAQIAA
                     GRAAAAAVSIAPEVLAYIVDLCRATRSSPSVSLGASPRGATALLRTSRAWAWASGRDF
                     VTPDDVKALARPTLRHRLALRPEAELEGATTDAVLEGVLATVPVPR"
     gene            complement(3693..4868)
                     /locus_tag="VGD55_02530"
     CDS             complement(3693..4868)
                     /locus_tag="VGD55_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0869249.1"
                     /translation="MTITQERAPTLHEGRSADPTIRQHWRRNRVLVGAGVVFVIGAVI
                     VALGSGTRSGDLDPRSFTGPGSHAIAVLLGQRGVHVTTATDAATADKLAVSGTTFVVV
                     SPDLLTTDELTTIASTHADLVVVGADIDQIQALGLPVADELDDGGTLRPPNCSLPAAT
                     VAGLAQLQGAGYSVPDGGQGCYPNGDGFALVTFETAGHRVTLLSDGTPLMNSALAKEG
                     NAALALGLLDTHPDVVWLVPPVIAPASSGSGTSSVTSLLPSRLRWAFLQLVIAVIVIA
                     LWRGRRLGRLVPEELPVVVRQAETVRGRSRLYRRSRSLDTAANALRDGTRDRLGHRLS
                     LGPRPQRAALVNAIASRTQQPAAQVDALLYGPMPSDDRALVTLAQALTALEQEVLRT"
     gene            complement(4865..5515)
                     /locus_tag="VGD55_02535"
     CDS             complement(4865..5515)
                     /locus_tag="VGD55_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY0869250.1"
                     /translation="MSLAVLVAVGTPPDPVTRAAAQAAARTELAKRAYHRGDPSLIDR
                     GLVWFLKKLGKLLENGARHAPGHQIGLLLILLILAALVIVVINRVGALRRSPHNDEAI
                     FGVEQTTADDHRRRADEFARTGAWAEAIRERLRAIARELEQRGVLDPRPGRTAAELSR
                     EAGAELPALADDLRNAASIFDRVWYGGRSATAADEELLRRLDSRVAGSHRGLAVSR"
     gene            complement(5512..6798)
                     /locus_tag="VGD55_02540"
     CDS             complement(5512..6798)
                     /locus_tag="VGD55_02540"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEY0869251.1"
                     /translation="MDDTPGWVDPTPPADSGPPQPPPPPAPPAQPPPAWQYFTSPAPP
                     PQGVGSSWGPPQYGPPQYGPPQSGSPQFGPPQYGPPQYGPPQYGPPQYQPPPYGQYVP
                     AWGGYQEYKPGVVPLRPLSVGEVLDGSFSTIRKHPRIVFGGAAVLAVVAELIRLLVGL
                     ALNNVPASLGSTSFSTNTDNTNGDTTANFSSIGSTATGAVVSLVVNALCVAVLAGVVT
                     GVVGKAVIGQQPNGREILGAVRKRWFGLLVVSVLAEVLPFTPILLLVFGILLGTISTG
                     AGVAGGVFGGLAALVLCPLLWGRLALAVPIFILERKGPGASIARSWRLVRGAFWRTWL
                     LRALVWIIVVVAGGTVSAPLFLIVFSSAASGKTPSTLDLIIAVIIGALVWMVTQPLTA
                     AALTIIYVDRRMRAEGLDLQLVQAARAAATPTPARA"
     gene            7045..>7397
                     /locus_tag="VGD55_02545"
     CDS             7045..>7397
                     /locus_tag="VGD55_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869252.1"
                     /translation="MKGRVLVVDDDTALAEMLGIVLRGEGIEPFFVFDGDKALAAFRE
                     TKPDLVLLDLMLPGTDGIDVCRQIRAESGVPIVMLTAKSDTVDVVVGLESGADDYVVK
                     PFKPKELVARLRARLR"
BASE COUNT         1256 a         2522 c         2441 g         1178 t
ORIGIN      
        1 gctcgcgatc aaagcgggtc cccagccctc gagcgggaac atgatgccgc gcacgagcgc
       61 gtgtcgaaac cgcgccggcc caccgtcgtc ccgcacaacg cgaaggccca gccaagcctt
      121 gccgagtgtg cgcccgcgcc acacggtctc gaggatcacg ttgtatccca cgaacacaaa
      181 gacgatgatg cacacgctga gcgctgccgc agtgtcgtcg tcggcgccgt cgaagagccg
      241 acccagcacc agcagcagaa tgaacagcag gatgaactgc gcaaccaggt cgacgagctt
      301 ggcgagcagg cgcgacggcc aatgtgcgag acggacgtcg agcagaacgg cctcgcctgt
      361 cacgactcca gcgctcatag tgccttagtc tgccggttgt ggacgtcgat gtgttcgttg
      421 ctgcacatca ggccagttgg gcgcgtctcg aagccctcgt ggagcgcgcc gatcgtcccc
      481 aacggctgac cggcgccgag gtcgacgaac tcatcgactt gtaccagcgc accgcgaccc
      541 acctgtcggt catccagtcc cgctcacccg atgcgcacct gatttcgcgg ctttcggtgc
      601 tcacagcacg cgcccgcgca gccattaccg gatcccacga tccggcgtgg agcgacttct
      661 cgcgattctt cgtcgtcaca tttcccgcgg tcttataccg gacggcgcgg tggtgggtct
      721 cgaccgcgct cgcatcatta gccctcatga cagccattgg cgtctggact gccacccacc
      781 gaggcgtggt cgattcgctg gtgtcaccgg ccgacgtccg ccaactcgtg tcgcacgact
      841 tcaaggatta ctactccgcc cacccggcga ccgatttcgc ggccaacgtg tggacccaca
      901 acgcgttcct taccggagcc acgcttgccc tcggcatctt cctcggcatc ccgaccatct
      961 atttgctctg gcagaactgc gtcaacgtcg gcgtcatcgg cggcattatg tcggcgaacg
     1021 ggaagctccc ggaattcttc ggcctgatcc ttccgcacgg attgctcgag ctcaccgcgg
     1081 tcttcatcgc gagcggtacc gggttgcgca tcggctggac gttaatcgaa ccaggcgccc
     1141 gaagacgcgc cgacgctctc gcgcaagagg gccgagcgtc gtttgcgatt gccctggggc
     1201 tcgtcgcgat gctcatggtg tcgggcgcga tcgaggcgtt cgtcacgccg tcgccattgc
     1261 cgacctgggc ccgcgtctcg atcggcgtga tcgtcgaact tgggttcttg acctacgtct
     1321 tcgtgctcgg ttcgcgcgcg gttcgcgcgg gagatattgg cgatctcgac gaagcgttga
     1381 acgcggacgt cgtcgaagcg accggctaac tacaacctgc cggccgcttt catcgccagg
     1441 tacgcatcgg aaaccctgct ggcgaactcg gccgggccgg catcgacaac ctcgacgtcg
     1501 agccgccgta ggagtgtggc gacccgccga tgcgctgcga gcgtcctctc tgccgcggct
     1561 gcgtcataga catcgcgcgc gtcgtcggac ctgtcaagca tcgcgagcac ctgcggatcg
     1621 gcaaccgacg cgagcatcac cacgtgcttg cgcgtgagcg acggcagcac tggcagcaat
     1681 ccttcggtga tcgcggcctc gtcaagcgtc gtgaacaaca cgacgagcgc gcgctggcgt
     1741 acgcgtcgca gtacctcggc gaccaatcgg cgtccatccg tctcgacgag gcgcggatgc
     1801 agcccggcca tcgattcgag cagacccgca agcgcgtcac cgtgcccctc ggagcccgac
     1861 gctgagccgc gggtcagcat gcgcggccgg tcgtcgaccg cgatgaggtc gaccttgtcg
     1921 ccggcgcgcg aggcgagggt gcctagcagc aacgcagcat cgatcgccgc gtcgagccga
     1981 ggtgctgcac cgatgcgcgc ggccgacgtg cgcccggtgt cgaggacgag cacgatccgg
     2041 cggtcgcgtt cgggccgcca ggtgcggacg acgacggctg agcgccgtgc cgttgcgcgc
     2101 cagtcgatcg accgcgcatc gtccccctcg acgtaatcgc gcagcgagtc gaactcggtg
     2161 ccctggccgc gcacccgtgc ggcggtgcga ccgtcgagaa tccgcagctt ggcaagcttt
     2221 tccggcaggt agcgccgact cggaaatgcc ggcagcaccc gcaggctcca cggcacctcg
     2281 tgattgccct ggcgtgccgc aagcccgagg gggccgacgc tgcgaacggt gacgcgtccg
     2341 gcctggagtt cggtgcgccg gccgggctgg atcgtggtga cccaccgacg acgttcaccg
     2401 gccgggacgt cgaccgcgcg gcgtgtcgga gaggccgaag cactcggagg ccaggcatcg
     2461 cgcaagacac cgcgcacccg acgtccaccc cgattgacga cctgaacccc gacctccgac
     2521 acctcgtcga gccggcgcgc ggtatctcca tatcgagaca cttcaagtgc gcgcacgctg
     2581 ccggccaacg cgagatcgac gcagatcgca acaacgagcg cgccctcgac gatcaccaca
     2641 gtgaaacccc gaacgggtgc ggcaaaaaca agtagcacgc cgacgagcgc gacgatcgcc
     2701 gcccgccgtg tcagcgccat gtggagctca cctcggcaca ggcaccgtgg cgagcacacc
     2761 ttcgagcacg gcgtcggtgg tcgcgccttc gagttcggct tctggccgca acgccagccg
     2821 gtgccgcaag gtcggccgcg caagtgcctt gacatcgtcg ggggtcacga agtcacgacc
     2881 cgatgcccac gcccacgcgc gcgacgtgcg aagcaacgcg gtcgctccgc gaggcgatgc
     2941 gccaagggac accgacggcg aactgcgggt cgcccggcac agatcgacga tgtacgcgag
     3001 cacctcaggc gcgatagaga ccgcagccgc ggcggcacgg ccggctgcga tctgggctgg
     3061 cccggcgaca gctaacaatc cggctcctgc aagatctcgc gggtcgaacc cgttcgcgtg
     3121 gcgcgtcagc atgccgaact cgtcgtcgcg acccggcagc ggcacgttga gcttcatcag
     3181 gaaccggtcg agttgcgcct cgggcagcgg ataggtgccc tcgtattcga ccgggttctg
     3241 cgtcgcaatc accatgaacg gttcgggtag cgggcgaggc gtgccgtcga cagaaacctg
     3301 gcgttcttcc atcgcttcaa gcagcgcggc ctgcgtcttc ggtggcgtgc ggttgatctc
     3361 gtcggcgagc agcaggttcg tgaagaccgg gccctgcctg aactcgaact cggcactgcg
     3421 tgagtcgtag atgagcgaac ccgtgacatc gcccggcatc aggtcggggg tgaactgcac
     3481 ccgcttggtc tcaagcgaca aggcggcggc gagggatcgc accagcaacg tctttgccac
     3541 gcccggcacg ccttcgagca atacgtgtcc gtccgcgagc agtgccacaa caagcccgct
     3601 gacgaccggg tcctgcccga cgaccgcttt cgcgacctca gcccgcaccg cggcgaacgc
     3661 ctcgcgcgac tgctcgatcg aactcgtcga cgtcacgtcc gaagtacctc ctgctcgagg
     3721 gcggtcagag cttgcgccag cgtcaccagt gcgcggtcat cgctcggcat tggaccatag
     3781 agcagcgcgt cgacctgcgc ggcaggctgc tgggtgcgcg acgcgatcgc gttcacgaga
     3841 gcggcccgtt gcggtcgcgg accgaggctc agccggtgac cgaggcgatc ccgagttccg
     3901 tcgcgcaggg cattggcggc ggtgtcgagc gatcgcgacc tgcgatacag ccgtgatcta
     3961 ccacgcacgg tctctgcctg tcggacgacg acgggcagct cttctggcac cagccggcca
     4021 agtcgccggc cgcgccaaag cgcgatcacg atgacggcaa tgaccagttg caagaacgcc
     4081 caccggagcc ggctcggcaa cagcgacgtc acgctgcttg tccctgaacc actcgacgca
     4141 ggtgcaatga cgggcggcac cagccacacg acgtccgggt gagtatcgag aagtcctagc
     4201 gcaagtgctg cgttcccctc cttggccagc gccgagttca tcagcggcgt gccgtcagag
     4261 agcagcgtca cgcgatggcc cgccgtctcg aacgtgacga gcgcgaagcc gtccccgttg
     4321 gggtagcagc cctgcccgcc gtcgggaacg ctgtagcccg caccttgtag ctgcgccaag
     4381 ccggcaaccg tggcggcggg aagcgagcag ttcggcgggc ggagcgtgcc accgtcatcg
     4441 agttcgtcgg ctaccggcaa tccgagcgct tggatctggt cgatgtctgc accgaccacg
     4501 accaggtcag cgtgcgtcga ggcgatcgtg gtcagctcgt cggtggtgag cagatcgggt
     4561 gacaccacga cgaacgtcgt gccactcacc gcgagcttgt cggcggttgc cgcatcggtc
     4621 gcggtcgtga cgtgcacgcc gcgttgcccg agcaacactg ctatcgcatg actgcccggg
     4681 cccgtgaacg agcgcgggtc gaggtcgccg ctgcgcgttc ctgaacccag cgccacgatg
     4741 acggcgccga tgacgaagac aacgccagca ccgaccagta cgcgattgcg ccgccaatgc
     4801 tggcggatcg tcgggtcggc gcttcgaccc tcgtgaagcg tgggcgcgcg ctcctgcgtg
     4861 atcgtcaacg gctcaccgcc aacccgcggt gtgatccggc aacacgggag tcgagtcggc
     4921 gaagcaattc ctcgtcagct gccgtcgcgc ttcggccgcc gtaccaaact cgatcgaaga
     4981 tggacgccgc attgcgcagg tcgtcggcca gcgcaggcaa ctcggcgccc gcttcgcgac
     5041 tgagttcggc tgccgtgcgc ccaggtctgg gatcgagcac gccgcgttgc tcgagttcgc
     5101 gcgcgatggc gcggagccgc tcgcggatcg cttcggccca cgcacccgtt ctcgcgaact
     5161 cgtctgcccg tcgccggtga tcatcagcgg tcgtttgctc gacaccgaag atcgcctcgt
     5221 cgttgtgtgg tgacctgcga agcgctccga cacggttgat caccacaatc accagcgccg
     5281 cgaggatcag caggatcagc agcaagccga tctgatgccc gggcgcgtgg cgggcgccgt
     5341 tctcaagcag cttcccgagc ttcttcagga accagaccag ccctcggtcg atcaacgatg
     5401 gatcgccgcg gtggtaggcg cgtttcgcca gctccgttct ggcggcggct tgtgctgcgg
     5461 cccgggtcac ggggtcgggt ggggtgccga cggcgaccaa gaccgccagg ctcatgcgcg
     5521 ggccggggtc ggtgtcgcgg ctgcccgtgc ggcctgcacg agttgcaggt cgagaccttc
     5581 ggcccgcatc cggcgatcaa cgtagatgat cgtcagcgcc gccgccgtca gcggttgggt
     5641 gaccatccac accagcgcgc cgatgatcac cgcgatgatg aggtcgagcg tcgagggcgt
     5701 cttgccgctc gccgccgacg agaacacgat gaggaacagc ggtgccgaca cggtgccacc
     5761 cgcaacgacg acgatgatcc agacaagcgc ccgcagtaac caggtgcgcc agaaagctcc
     5821 gcgtaccagg cgccacgatc gggcgatcga tgctcccggg cccttgcgtt ccaagatgaa
     5881 tatcgggacg gcgagtgcta gccgtcccca caacagcgga caaagcacaa gtgcggcaag
     5941 accgccgaac accccgccgg cgacacctgc gccggtactg atggtcccga gcagtatgcc
     6001 gaacaccagc agcaggatcg gcgtaaacgg caacacttcg gcaagtacgg agaccacaag
     6061 cagcccgaac caccgcttgc gaaccgcccc caaaatctcg cgcccgttgg gctgctgccc
     6121 gatgaccgcc ttgcctacga cgccggtcac gacgccggca agcactgcca cgcacagagc
     6181 gttcactacg agcgacacca cggcgccggt ggccgtactg ccgatcgagc tgaagtttgc
     6241 ggtcgtgtcg ccgttggtgt tgtcggtgtt ggtcgagaac gacgtcgagc ctagggaggc
     6301 gggcacgttg ttcagcgcga gccccacgag gagacggatc agttcggcaa cgacggcgag
     6361 cactgcggcg ccgccgaaaa cgatccgcgg gtgcttgcgg atcgtggaga agctgccgtc
     6421 gagcacctca ccgaccgaca gcggtcgcag cggtaccacg ccaggcttgt attcctgata
     6481 gccgccccac gccggcacgt attggccgta gggcggcggt tggtactgcg gcggcccgta
     6541 ttgcggtggc ccgtactgcg gtggcccgta ctgcggcggc ccgaactgtg gggagccgga
     6601 ttgcggtggc ccgtattgcg gcgggccgta ttgcggtggc ccccatgacg accccacgcc
     6661 ctgaggcggc ggagccggcg aagtgaagta ctgccaggcg ggtggaggct gcgccggagg
     6721 tgccggcggc ggtggtggct gcggcggccc ggaatccgcc ggcggggtcg ggtcgaccca
     6781 gccgggtgtg tcatccatgg gttctcccgt aatcgcaaac gctcacactc acccgctcgc
     6841 cgacgggttc atcgtcccac ggcctagcgt cgcaatgcgg tcgattcgcc cgcgacgcgg
     6901 cgtccgccct gctcctaccg tgctcgtaac gtgcccttac ccactcacgt cgatagggaa
     6961 gcgcattcaa ctcgtgaccg cgcgaacccc tttgaggtga caccatgggc tcatgaccgt
     7021 gttgagcgct ctgggggcta catcatgaag ggccgcgttc tggtcgtcga cgacgacacc
     7081 gcgcttgctg agatgctcgg catcgttttg cgtggggagg ggatcgaacc gttcttcgtc
     7141 tttgacggtg acaaggcgct cgcggcgttt cgggagacca agcccgactt ggtgctcctc
     7201 gacctgatgt tgcctggcac cgacggcatc gacgtgtgcc ggcagattcg cgccgagtcc
     7261 ggcgtcccga tcgtgatgtt gaccgcgaag agcgacaccg tcgatgtggt cgtcgggctc
     7321 gagtcgggtg ctgacgatta cgtcgtcaag ccgttcaagc ccaaggaact ggtcgcccga
     7381 ttgcgggcca ggttgcg
//
LOCUS       DASWVF010000147         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_131477, whole genome shotgun sequence.
ACCESSION   DASWVF010000147 DASWVF010000000
VERSION     DASWVF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131477"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGD55_02550"
     CDS             complement(<1..385)
                     /locus_tag="VGD55_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0869253.1"
                     /translation="MTPSSDVSTSVDGAAAYLSLGVGYRHNAMRAADWVALRDAASAL
                     GADESVNVVVIRGAGGTFSAGSDIREWAGADSADIDASFHLMEQALSAVERIPIPTIA
                     LVEGVAAGAGCQLALSCDMCVMADSA"
     gene            complement(411..1289)
                     /locus_tag="VGD55_02555"
     CDS             complement(411..1289)
                     /locus_tag="VGD55_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0869254.1"
                     /translation="MRLTAYRDGLHTIVACRTSDGGVAPLAEAEEFWADPYGWLAHPN
                     ATATSRDVSELQIVPAVRGSARILCVGLNYQAHADEGPFTAPTTPTVFGRWAASLSTS
                     GSSVRVPPDEPGLDWEGELVVVVGRPLSEATVAEAESAVFGFAAFNDITARSAQRLST
                     QWTLGKNVDRSGPLSDIVTADEVGDISDGLLLTTQVNGETVQSARTTEMIFSVEQLLA
                     FISRTMTLHPGDIVATGTPSGVGYARTPPWFLNAGDTVVVDIERVGSVTTNVVASTSA
                     SDSESELASVADGVTS"
     gene            complement(1286..>1660)
                     /locus_tag="VGD55_02560"
     CDS             complement(1286..>1660)
                     /locus_tag="VGD55_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0869255.1"
                     /translation="ASILVRPERMRVHPLGTGSADNILQGRLQSVTYLGPARRLEVAV
                     PGLNDVIVREPTNLGPPLTVGDGVDVVWSARDSWLIPAAESGDAEPLASGGQSAAAVG
                     ALLEQSDTAEPARVPDLGDGRR"
BASE COUNT          291 a          582 c          530 g          257 t
ORIGIN      
        1 gcgccgaatc ggccatcacg cacatgtcac aactcagcgc cagttggcag cccgcgcctg
       61 ccgctacgcc ttcgaccagc gcgatcgtcg ggatcgggat ccgttcgacc gcactcagcg
      121 cctgctccat gaggtggaag ctcgcgtcga tgtccgccga atcggcgcca gcccattccc
      181 tgatgtcgga gccggcactg aacgtgccgc ccgcgcccct gatcacgacg acgttgaccg
      241 actcgtctgc ccccaacgcc gaagccgcgt cgcgcagtgc gacccagtca gccgcgcgca
      301 tcgcgttgtg ccggtagccg accccgagcg agaggtacgc cgccgcgccg tcgaccgatg
      361 tcgatacgtc gctgctgggc gtcaccaagg cgggatccgg ctcgccccgg tcagctggtg
      421 acaccatcgg caaccgaggc gagctcagac tctgaatcgg acgccgaggt gctggccacg
      481 acattcgtcg tgaccgaccc gacgcgctcg atgtcaacga caacggtgtc gcccgcgttg
      541 agaaaccacg gtggggttcg tgcgtaaccc acgccggacg gcgtgccggt cgcgacgatg
      601 tctccggggt gcagcgtcat cgtgcggctg atgaaggcca gcagctgctc gaccgagaaa
      661 atcatttcgg tcgtgcgcgc cgactgcacg gtttcgccgt tcacctgagt cgtcagcaac
      721 agaccgtcgg agatatcgcc aacctcgtca gcggtgacga tgtcactgag cggcccgctt
      781 cgatcgacgt tctttccgag cgtccactgc gtcgacaatc gctgcgccga tcgagcggtg
      841 atgtcgttga aggcggcgaa tccaaacacc gccgactcgg cttccgcaac cgtcgcctcg
      901 gagagtgggc ggccgacgac gacgacgagt tcaccctccc agtcgagacc cggctcgtcg
      961 ggcggcactc gtaccgacga accactcgtc gacaacgacg cggcccaacg cccgaagacc
     1021 gtcggcgtgg tcggcgcagt aaacgggccc tcgtcggcgt gcgcttgata gttgaggcca
     1081 acacacagaa tgcgggcaga cccccgaacg gccggcacaa tctgcagttc cgaaacatca
     1141 cgcgatgtcg ccgtcgcatt cggatgcgcg agccacccgt aggggtcggc ccagaattct
     1201 tccgcctcgg cgagtggcgc gacgccgcca tccgacgtcc ggcacgcgac gatcgtgtgg
     1261 agtccgtcgc gatatgcagt gagcctcatc ggcgaccgtc gccaagatct ggtacccgag
     1321 ctggctcggc agtgtcgctc tgctcgagca gcgcgccgac cgccgcagcg gactggccgc
     1381 cgctagccaa cggctctgca tcgccagatt cggcggcggg tatcagccac gaatcgcgag
     1441 cgctccagac aacgtcgacg ccgtcgccga cagtcagcgg cggcccgagg ttcgtcggct
     1501 cgcggacgat cacatcgttg agaccgggaa cggcaacctc aaggcgccgg gccgggccga
     1561 ggtacgtcac gctttgcagg cggccctgca agatgttgtc tgcagagcct gtgcccaggg
     1621 gatgcactcg catccgttcg ggccgcacca ggatgctcgc
//
LOCUS       DASWVF010000148         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_335144, whole genome shotgun sequence.
ACCESSION   DASWVF010000148 DASWVF010000000
VERSION     DASWVF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335144"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VGD55_02565"
     CDS             <1..1569
                     /locus_tag="VGD55_02565"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY0869256.1"
                     /translation="GSDPHTTAGKLAELRRLDAAALHAGSAAAVERQHAKGKGTARER
                     IDALLDPDSLVELDRLARHRSNAFDLPANRPYGDGVITGHGTIDGRPVCVFSQDFTIF
                     GGSLGEVFGEKIVKVMDLAMKGGWPVIGINDSGGARIQEGVVSLGLYAEIFYRNTIAS
                     GVIPQISLVLGPCAGGAVYSPAITDFIIMVDKTSHMFITGPDVIKTVTGEDVEFEELG
                     GARTHNARSGVAHYLATDEADAIDYAKALLSYFPSNNVDDPPVTPTEISMDVTPTDIA
                     LDTLVPDSANQPYDIHKVVEAVLDDGEFLETQALFAPNIVCGLGRVEGHSIGVVANQP
                     MHFAGCLDIDASEKAARFVRTCDAFNIPILTFVDVPGFLPGTDQEWNGIIRRGAKLLF
                     AYAEATVPKITVITRKAYGGAYDVMGSKHLRADVNLAWPSAQIAVMGAQGAVNILYRT
                     ELAAADDPQAQRAAYVTEYEDHLANPYIAAERGYVDAVIAPSQTRVEVTRALRMLRNK
                     RDQLPPKKHGNIPL"
BASE COUNT          299 a          521 c          501 g          268 t
ORIGIN      
        1 gggtctgacc cgcacacgac agcaggcaag ctcgccgagc tccggcggtt agatgccgcc
       61 gctttgcacg cgggctcggc ggccgcggtc gagcgtcagc acgcgaaggg caagggcacc
      121 gcacgcgagc gcatcgatgc gctgctcgac cccgactcgc tcgtcgaact ggatcgcctc
      181 gcacggcacc ggtcgaacgc gttcgatctc cccgcgaatc ggccgtacgg cgacggggtc
      241 atcaccgggc acggcaccat cgacggccgg ccggtctgcg tgttctccca ggacttcacg
      301 atcttcggcg gaagccttgg tgaagtcttc ggcgagaaga tcgtgaaggt gatggatctg
      361 gcgatgaagg gcggctggcc ggtgatcggt atcaacgact ccggcggcgc gcggattcag
      421 gaaggcgtcg tgtctctcgg cctttacgcc gagatcttct accgcaacac gatcgcgtct
      481 ggcgtgatcc cgcagatctc gctcgtgctc ggaccttgcg cgggtggcgc ggtgtactcg
      541 cccgcgatca ccgacttcat catcatggtc gacaagacct cgcacatgtt catcaccggt
      601 cccgatgtca tcaagaccgt caccggcgag gacgtcgagt tcgaagaact cggaggtgcg
      661 cgcacccaca acgcacgctc gggtgtcgcg cactacctgg cgaccgacga ggctgacgcg
      721 atcgactacg ccaaggcact gctgtcgtac ttcccttcga acaacgtcga cgacccgccg
      781 gtaacgccta ccgagatttc gatggacgta accccgaccg acatcgcgct tgacacgctc
      841 gtgcccgact cggccaacca gccgtacgac atccacaagg tggttgaggc ggtgctcgac
      901 gatggcgaat tccttgagac gcaagctctt ttcgcgccga atatcgtgtg tggcctgggt
      961 cgcgtcgaag ggcattcgat cggtgtcgta gcgaaccagc cgatgcattt cgcggggtgc
     1021 ctcgacatcg atgcgtcgga gaaggcggct cgtttcgttc ggacgtgcga cgcgttcaac
     1081 atcccgatcc tcacctttgt cgacgtgccc ggcttcctgc ccggcaccga ccaggaatgg
     1141 aacggcatca tcaggcgcgg agcaaagttg ctgtttgctt acgccgaagc gacagttccg
     1201 aagatcaccg tcatcacccg caaggcatac ggcggcgcgt acgacgtcat gggcagcaag
     1261 catctgcgcg cagacgtgaa cctggcgtgg ccgagcgcgc agatcgcggt catgggcgcc
     1321 cagggggcgg tgaacatcct gtaccgcacg gaattggcgg ctgctgacga ccctcaagcg
     1381 cagcgagctg cgtacgtgac ggagtacgaa gatcatctcg cgaaccccta catcgccgcc
     1441 gagcgcggat acgtcgacgc cgtcatcgcg ccgtcgcaga cgagagtcga ggtcacccgc
     1501 gccctgcgca tgctgcgaaa caagcgcgac cagttaccgc cgaagaagca cgggaacatc
     1561 ccgctgtgag cgagccgatc cgcatcgcc
//
LOCUS       DASWVF010000149         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_29570, whole genome shotgun sequence.
ACCESSION   DASWVF010000149 DASWVF010000000
VERSION     DASWVF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29570"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGD55_02570"
     CDS             complement(<1..516)
                     /locus_tag="VGD55_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869257.1"
                     /translation="MIADSFPRLQARTARFTLGRPRGFVVSPDGARVLFLRSRGGTDR
                     VTCLWSYDVATGIESLLADPSELLAAGGDRADLPATEKARRERAREQAGGIVGFSTDA
                     QVGVAAFTVVGRLFVTALIGESSARNDDSGAAVRELTVGGPVFDPRLDPTGRAVAYAS
                     NGTLRLAGVDNP"
     gene            596..1450
                     /gene="mshB"
                     /locus_tag="VGD55_02575"
     CDS             596..1450
                     /gene="mshB"
                     /locus_tag="VGD55_02575"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HEY0869258.1"
                     /translation="MTQRSLLLVHAHPDDETIGTGATMAKYAAEGVNVTLVTCTLGEE
                     GEVLVPALAGIAADKADQLGGWRMHELEEACKALGVTDHRFLGGPGGYRDSGMMDTPA
                     NENPRCFWRADLDVAAAQLIEVIREVRPQVVVTYDEFGAYGHPDHIQAHRVTVKALEQ
                     TRDIVAKFYYTAVPRSFIEASIVAMKEQGHEFFGGVESADDVPFAKSDDEITTQIDAR
                     DFLDAKVEAMRAHATQIEVDGPFFALSNHIGQKAFGLEHYVLMAGEKGPTDASTGHDW
                     EIDLFAGL"
BASE COUNT          265 a          489 c          523 g          239 t
ORIGIN      
        1 cgggttatcc acgccggcca atcgcagggt cccgttgctg gcataagcga ccgcgcgacc
       61 tgtcgggtcg agtcgtgggt cgaacacggg gccgccgacc gtgagctcgc ggaccgctgc
      121 gccgctgtca tcgttgcgtg cggagctttc accgatgagc gccgtcacga agaggcggcc
      181 aaccacggtg aatgccgcga ccccgacctg cgcgtcggtc gaaaatccca cgatgccgcc
      241 ggcctgttcg cgggcccgct cgcgtcgcgc cttctcggtt gcaggtaggt ctgcccgatc
      301 gccaccggcg gccagcaact cgctcgggtc ggccagcagg ctctcgatcc cggttgccac
      361 gtcgtagctc cacaggcagg tgacccgatc ggtgccacca cgcgagcgca ggaacagcac
      421 gcgcgcgccg tcgggcgaga cgacgaagcc ccggggtcgc ccgagcgtga agcgggcggt
      481 gcgtgcttgc aggcgcggga acgagtcggc gatcacctgc cagatcctgc catctgtgtc
      541 gtgctcgctg ccccgctggc gccgagctgt caggcgtccc ccgtagggtt aacgcatgac
      601 acagcggagc ctcttactcg tccatgccca tcctgacgac gagaccatcg gtactggcgc
      661 gacgatggcc aagtacgcgg ctgagggcgt caacgtcacc ctggtcacct gcacgctcgg
      721 cgaagagggc gaagtcctgg tgccggcgct cgcaggcatc gccgccgaca aggcagacca
      781 gctcggtggt tggcggatgc acgagctcga agaggcgtgc aaggcgctcg gcgtcacgga
      841 tcaccgcttt ctaggcggtc ctggcggcta ccgcgactcc gggatgatgg acacccccgc
      901 caacgagaac ccgcgatgct tttggcgggc cgaccttgac gtggcggcgg cgcagctgat
      961 cgaggtgatc cgcgaggtcc ggccgcaggt cgtcgtcacc tacgacgagt tcggggcata
     1021 cggccacccc gatcacatcc aggcgcaccg ggtcacggtc aaggctttgg aacagacccg
     1081 cgacatcgtg gcaaagttct attacacggc cgtgccgcgc tcgttcatcg aggcgagcat
     1141 cgtggcgatg aaggagcagg ggcacgagtt cttcggtgga gtcgagagcg ccgacgacgt
     1201 gccattcgcg aaatccgatg acgagatcac gacgcagatc gatgcacgcg actttctcga
     1261 cgcgaaagtc gaagcgatgc gcgcccacgc aacgcagatc gaggtcgacg ggccgttctt
     1321 cgcgctgtcg aaccacatcg gccagaaggc gttcggtttg gagcactacg tcttgatggc
     1381 cggcgagaag ggtccgaccg acgcgagcac gggtcacgat tgggagattg acctgtttgc
     1441 ggggttgtga gccatccggg tgaaccgact agcggcggtg cggaccggct gagagactgc
     1501 agaagtcgca gttggg
//
LOCUS       DASWVF010000150         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_80339, whole genome shotgun sequence.
ACCESSION   DASWVF010000150 DASWVF010000000
VERSION     DASWVF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80339"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="dacB"
                     /locus_tag="VGD55_02580"
     CDS             <1..840
                     /gene="dacB"
                     /locus_tag="VGD55_02580"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0869259.1"
                     /translation="TTTVSLGYDASLFVGPQLAPGWKPVYQTEGDVAPVSALEVDEGS
                     QDLTKPGRVADPASAAAGQFAALLKVYGITVTGAPRADKAPNTPITLAAVASPPLYEL
                     VERMLGRSDNDLAEALARQVAIASGMPASFAGGVHAVKAALTAAGINAAALSMVDASG
                     LSPLDRLTPALLAQLLRTALVPAPDRTDLSAMITGLPVAGFYGTLAGRFKASAAAGAG
                     VIRAKTGTLNGVVSLAGYLPDASGATLVFAVVVNGVKLNAADVTEVALDRILASLPGC
                     GCQ"
     gene            862..2019
                     /locus_tag="VGD55_02585"
     CDS             862..2019
                     /locus_tag="VGD55_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY0869260.1"
                     /translation="MTETLRLIDWDLATSTACRLAPGGPSVTRDEAFDAVRKLRALAV
                     EAEEHVAAFTGMAGPHVHGPAFVTDRTGWIKGNVGGFQVVMKPLLDKMATKQASKVAA
                     PVVTAVGSRVTGVQVGLLLAYLSGKVLGQYELFLPPVDGHPQAGRLTLVAPNIVGAER
                     QLDVDPRDFRLWVCVHEVTHRTQFTAVPWLRSHLQDEISAFLLASDLDPAAMLGRLRD
                     VADSVRDNMRGKTSDNDGGLSLLEAVQNPEQRAVLNRLTAVMTLLEGHGEYVMNGVGP
                     SVIPSVEEIGRKFGERRRGQSPADRLVRKLFGLDMKAKQYAEGAAFVRAVVERAGMAS
                     FNRIWTSPNTLPTKAEIAKPELWMSRVLRMTVEADSGEVGNQGGGSGDGGD"
BASE COUNT          334 a          677 c          688 g          321 t
ORIGIN      
        1 accaccaccg tcagcctcgg ctacgacgcg tcattgttcg tcgggccgca gttggccccg
       61 ggctggaagc cggtctatca aaccgaaggt gacgtcgcgc cagtgtcggc actcgaggta
      121 gacgagggca gtcaagacct cacgaagccg ggccgagttg ccgatccggc gtcggcagct
      181 gctggccaat tcgccgcgct gttgaaggtc tacggcatca cggtcacagg cgcgccgcgg
      241 gccgataagg ctccaaacac gccgatcact ctcgcagccg tcgcatcgcc accgctgtac
      301 gagctggtcg agcggatgtt ggggcgcagc gacaacgacc tcgccgaggc gcttgcccgt
      361 caggtcgcga tcgcgtcggg catgcccgca agcttcgccg ggggtgtgca cgcagtgaag
      421 gcggcgctga ccgccgccgg cataaacgcc gcagcgttgt cgatggtcga cgcgagcgga
      481 ctctccccac tcgaccggct caccccggcg ctgctcgcac aactactgcg caccgccctg
      541 gttcctgcgc ccgaccgcac agacctgtcc gcgatgatca cgggcctgcc cgtcgcaggc
      601 ttctacggca cgctggctgg ccgcttcaaa gcttcggccg cagcgggcgc gggagtgatc
      661 agggcaaaga ccggcacgtt gaatggggtc gtctcgttgg cgggctatct ccctgacgcg
      721 agtggcgcga cgctggtctt cgccgtcgtc gtgaacggcg tcaagctgaa cgcagctgac
      781 gtcaccgagg tcgcgctcga ccgcatattg gcgagtctcc ccggctgcgg ctgccagtaa
      841 ctgcgccgta cggtagggcc catgaccgag acgctgcggc tgatcgactg ggaccttgcg
      901 acctccaccg cgtgccgtct tgccccgggc ggcccgagcg tcacgaggga cgaagccttc
      961 gacgcggtcc gcaaactgcg agctcttgcg gtcgaggcgg aggaacatgt tgcggcgttc
     1021 accggcatgg caggcccgca cgtccacggg cctgcgttcg tcaccgaccg caccggctgg
     1081 atcaagggca acgtcggtgg gttccaggtc gtgatgaagc cgctgctcga caagatggct
     1141 acgaagcagg cgagcaaggt cgcggcgccc gtcgtgaccg ccgtcggttc gcgggtcacc
     1201 ggcgtgcagg tcggtctgct gctggcctac ctatcgggca aggtgctcgg ccagtacgag
     1261 ctgttcctgc cacccgtcga cggacacccg caggccggtc gcctcacatt ggtcgcaccc
     1321 aacatcgtgg gggccgagcg gcaactcgac gtcgatccgc gcgacttccg gctctgggtc
     1381 tgcgtgcacg aggtgacgca tcgcacgcag ttcaccgccg tgccgtggtt gcgctcgcac
     1441 ctgcaagacg agatcagtgc gttcctgctc gccagtgacc tcgaccccgc ggcgatgctt
     1501 ggccggttgc gcgacgtcgc cgacagtgtg cgcgacaaca tgcgcggcaa gacctccgac
     1561 aacgacggcg gcctgagcct gctcgaggcc gtgcagaacc ccgagcagcg cgccgtgctc
     1621 aaccgactca ccgccgtgat gacgctgctc gaagggcacg gcgagtacgt gatgaacggg
     1681 gtcggccctt cagtgatccc gagcgtcgag gagatcggcc gcaagttcgg cgaacgccgg
     1741 cggggtcaga gtccggccga tcgcctcgtg cgcaaactct tcggtctcga catgaaggcc
     1801 aagcagtacg cagaaggcgc tgcgtttgta cgggcggtgg tcgaacgcgc gggtatggcg
     1861 tcgttcaacc gcatctggac ctcgcccaac acgttgccca ccaaggccga gatcgccaag
     1921 cccgagttgt ggatgagccg ggtgcttcga atgacggttg aagccgactc cggcgaggta
     1981 ggcaatcagg gcggcggctc gggtgacggc ggcgactgaa
//
LOCUS       DASWVF010000151         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_386365, whole genome shotgun sequence.
ACCESSION   DASWVF010000151 DASWVF010000000
VERSION     DASWVF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386365"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1365)
                     /gene="ftsY"
                     /locus_tag="VGD55_02590"
     CDS             complement(184..1365)
                     /gene="ftsY"
                     /locus_tag="VGD55_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0869261.1"
                     /translation="MQPWIEIVLAVVVLAGAGGAFFVRGSRRGGGRVQPPGPEPVARP
                     TKATTAAPPRGDTGVEAEPATDEATTEAITEAITEAITEAPEPAAGRLIRLRARLARS
                     ESTLGRGLLALLSRDTLDDATWQEVEDLLITADVGVAASNELVASLKSKARVLGTRSA
                     GDARALLREGLLELVDPDLDRSLATRREPGEGPAVLLVVGVNGTGKTTTCGKIARLLV
                     AEGRTVVLGAADTFRAAAADQLETWGRRVGAQTVRGPEGGDPASVAFEAVKTAIDLGA
                     DTVLIDTAGRLHTKAGLMDELGKVKRVVEKQSEVDEVLLVLDATTGQNGLVQARVFAE
                     VVAITGVVLTKLDGTAKGGIVISVQRELGVPVKLIGLGEGADDLAPFEPEAFVDALLG
                     S"
     gene            1459..2127
                     /locus_tag="VGD55_02595"
     CDS             1459..2127
                     /locus_tag="VGD55_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869262.1"
                     /translation="MGLRRTAFAAATISLAMLAGCSAASHPSALGPAASAPIAVAPSV
                     GPSAPPNSVAASPQPSASASASTESASTAGTPTTAADSPPPALSSNDVAANVRATAQA
                     FFADLNIAFATGDLTSYNTLTSPGCVCRSIARTIQDMNAEHEHYSGVVATITSLQVVS
                     FIAAGATADIYFSVSAGKVLDAAGKQVDTSLAAPAAHSAMFILRVGPHWIVEQNTRLD
                     PATP"
     gene            2463..>2834
                     /locus_tag="VGD55_02600"
     CDS             2463..>2834
                     /locus_tag="VGD55_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869263.1"
                     /translation="MITNPALPASTVGATQCRSASGATPAQVQQAAFDAFSQLLTTAH
                     PTQQPANGGIVNLPTLFATNTPPAQTFNETLLGVQVTLNVEASWTWHFGDGSTLTSRN
                     AGGAYPITTLSHTYLTAGEDTI"
BASE COUNT          496 a          986 c          864 g          488 t
ORIGIN      
        1 tgatcaattt catgaactca ccttctcagt tgcgtcggca tgttccgcat gcggatgaat
       61 ggaatgtcgt gtcactacgc ggggagcaaa cttgccgaca ccgatttccc tgaggggact
      121 cggctgtttc acagcggtga aatcaccggc cttgacgtta cgtgcgtgta aaccattctg
      181 aggtcaggag cccagaagcg cgtcgacaaa agcctctggt tcgaacggcg cgaggtcgtc
      241 ggcaccttct cccagaccga tgagcttgac cgggacgccg agttcgcgct gcacagagat
      301 aacgatgcca cccttcgccg tcccgtctag cttggtcagg acgacgccgg tgatcgcgac
      361 cacttccgcg aaaacacgcg cctgcacgag cccgttttgt ccggtcgtcg cgtcgagcac
      421 gagcagcacc tcgtcgacct cgctctgttt ctcgacgacc cgcttcacct tgcccagctc
      481 gtccatgagg ccggccttgg tgtgcagccg accagcggtg tcgatcagca cggtgtcggc
      541 gccgaggtcg atcgccgttt tgacggcctc gaacgcaacc gacgccgggt cgccgccttc
      601 gggtccgcgc acggtctgcg caccgacccg acgtccccac gtttcgagct ggtcggcggc
      661 agcggcgcgg aatgtgtcag cagccccgag aacgaccgtg cggccttcgg cgacaaggag
      721 ccgggcgatc tttccgcagg tcgtcgtctt gccggtcccg ttaacaccaa cgaccaggag
      781 cactgcgggc ccttcgccgg gctcacgccg tgtcgccagc gatcgatcga gatcaggatc
      841 gacgagctcg aggagccctt ccctgagcag tgcacgtgcg tcgccggccg atcgcgtgcc
      901 gagtacccgt gccttcgatt tcaaggatgc gaccagttcg ttacttgccg cgactccgac
      961 atcggcggtg atcaggaggt cctcgacctc ctgccaggtc gcgtcgtcga gcgtgtcgcg
     1021 tgacagcagc gcaagaaggc cgcggccaag ggtcgactcc gaccgtgcca gccgggcgcg
     1081 cagccgaatg agccgcccgg cggcgggctc cggcgcctcg gtgatcgcct cggttatcgc
     1141 ttcggtgatg gcttcggttg tggcctcgtc ggtagccggc tcagcctcaa cgcctgtgtc
     1201 gccgcggggt ggcgctgcgg tggtcgcctt cgtgggcctc gcgaccggct ccggcccggg
     1261 cggctggacc cggccgccac cacgccgact gcctcgcacg aagaatgcgc cgccggcgcc
     1321 cgcgaggacg acgacggcca gaacgatctc gatccacggc tgcacgggcc caagtctcgc
     1381 attcgacccc tgcggccatg cgggttcacc ttgtggataa cccagaacgg cgcgctcgcc
     1441 tcccgtactg tcgctcgcgt ggggcttcgc cggacggcct tcgctgcggc gacgatcagc
     1501 ctcgccatgc tggccggctg ctcggctgca agccatccca gcgcgctcgg ccccgcagcc
     1561 tccgccccca ttgcggtcgc gccttccgtc ggcccgagcg ctccgccgaa ttcggtcgcc
     1621 gcaagccctc agccatcagc gagcgccagt gcttcaaccg aaagtgcatc aacagccggg
     1681 acgccgacca ccgccgccga ctcgcccccg cccgccctgt cgtcgaacga cgtcgcagcc
     1741 aacgtccgcg caacagcgca agccttcttc gcggatctca acatcgcctt cgccacgggt
     1801 gacctgacta gctacaacac cctgacgtct ccgggctgcg tgtgccgcag tatcgcccgg
     1861 acgatccaag acatgaacgc cgagcacgag cactacagcg gcgtcgtcgc gacaatcaca
     1921 tcgcttcagg ttgtgagttt catcgcagca ggcgccaccg ccgacatcta cttttcagtc
     1981 tcggcgggca aggtactcga cgcagcgggc aaacaagtcg acacatccct ggccgctcca
     2041 gcagcccatt cggctatgtt catccttcgc gtcggcccac attggatcgt cgagcagaac
     2101 acgcggctcg acccggcaac gccatgaccc gcctctccgc gttcgtatgc gcattgacca
     2161 tcgccacgtg cttcaccgcc ttcagtagcg gatctgccga ggcaagccca ccgaactcgc
     2221 tctcgggcgg atatgccgcc gaagtcggca acccagcggg tggtattgat ggggacagtg
     2281 gaagtggcac ggtagatggg accgggagtc agggcagcga tcctgcatcc ggcagccagg
     2341 gcgtctcgtc gcctgacaac tgcaaagggt gcacctacaa gtgggttagt gtctgcgacc
     2401 cggcgcttgg gaacggtggc tgcgcgaatg catgtccggc tggcttcgcg ttggaatcgc
     2461 tcgtgatcac aaaccctgcg cttccggcat caacagttgg cgctacccag tgccgatcag
     2521 cgagtggtgc gacgccggcg caggtacaac aagccgcatt cgacgcattc tcgcaattgc
     2581 tgactacggc tcatccaacg cagcaaccgg cgaacggcgg gatcgtgaat ctgccgacgc
     2641 tgttcgccac caacacacca ccggcgcaaa cgttcaacga aacgttgctc ggcgtgcaag
     2701 taacgctgaa tgtagaagcg tcatggacgt ggcacttcgg cgacggctca acgctgacat
     2761 cgagaaacgc cggcggggcg tatccgatca caacgttgag ccatacctac ctcaccgcgg
     2821 gcgaggacac catc
//
LOCUS       DASWVF010000152         6118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_437380, whole genome shotgun sequence.
ACCESSION   DASWVF010000152 DASWVF010000000
VERSION     DASWVF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437380"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGD55_02605"
     CDS             <1..712
                     /locus_tag="VGD55_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0869264.1"
                     /translation="ISLDGAVAEITRWIDDGEHHYVCVTNVHTVIECQHDEELRRIHN
                     ESGLTTPDGMPIVWCAKRAGADGVTRVYGPDLMQALSKSLAQSGSSVFLYGTTARTLE
                     LLTARLISDFPGLRIVGSYAPPFRPLTPTEDAEIVRRINYSGADVVWVGLGAVKQEYW
                     MAEHIDVLHAHALIGVGAAFDFHAGVVKQAPLWMQRRGLEWFYRLCREPRRLWRRYLR
                     TNPAFVLGVLRRPPRLRS"
     gene            complement(702..1916)
                     /locus_tag="VGD55_02610"
     CDS             complement(702..1916)
                     /locus_tag="VGD55_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0869265.1"
                     /translation="MTNSVRVAVDARLFTGASGGVETVLVGLADGLSAIEHPDVDISF
                     VAYTGHTEWLARLIGESIQLIEVQPPHKPSPQLRRSLGPLAEPARRLRNSIKGVHTRL
                     PHDHQLDALKPDVVHLPHQEVGLVAAPFIYHPHDLQHLHMPEFFSKETLTRRETFYGP
                     LCRAAARVAVGTSWVKDDVVAKLGIEPAKVVVIPLAPVATVTASTQSPAGLPASYLLY
                     PAAAWPHKNHMRLLQAFRILVDAHPDAHLVLTGARTGVDLTANVRDLGLSDHVHVLGF
                     LPERDLAAVYQHARGVVVPTLFESASFPVWEAFERGVPVACSSVTALPRQVGDAAIVF
                     DPYDVEEIADAMSQLWTDPQLRAALITQGKLRVSEFSWLDTARRFLALYREVAGVATA
                     ADVSLLAQEPKI"
     gene            complement(1936..3039)
                     /locus_tag="VGD55_02615"
     CDS             complement(1936..3039)
                     /locus_tag="VGD55_02615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0869266.1"
                     /translation="MTAIPLARPDLSPRETAYVTDAIHTSWIASAGPYLERFESEFAE
                     MCGTRHALAVSNGTVALHLALLGLGIGPGDEVIVPSLTYIASVNAVTYVGARPVFVDV
                     DPVTWCIDPAAVALAVTPQTRAIMAVHLYGQPADMDALRSIARAHALHVVEDAAEAPC
                     ASYGGRPTGSLGDVATFSFYGNKILTSGEGGALTSDDDDLTARMKLFRGQGMDLNRRY
                     FFPVIGYNYRLTNIAAALLCAQMERRHELIERRTAIFARYTEGLRDIRGLELQADVAG
                     QTRALWLYSVLVGEEFGRTRDDLAAALAEVEIETRPFFIPVHTLPPYASASTSNLSVT
                     MRLARDGINLPTFSQMTDVDVDKVCAAIASAAR"
     gene            complement(3036..3641)
                     /locus_tag="VGD55_02620"
     CDS             complement(3036..3641)
                     /locus_tag="VGD55_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY0869267.1"
                     /translation="MSQPLFLLGNGGHAPEVALIARAMDPLGERWRELVFLGPGDEDR
                     ALADGGEAALAVGTPTLRLHLWEKYCDRPGLEWPILIHPRADVGPTSTVGRGACISSG
                     SVITTNVVIEEAALINFNVTIGHDCRIGAGTVVNPGAAVSGDCHIGRGCLLGAGAVVL
                     EHLVVEAGAVVGAGAVVTKDVAAGTTVVGIPARPLNRGPEQ"
     gene            complement(3674..5050)
                     /locus_tag="VGD55_02625"
     CDS             complement(3674..5050)
                     /locus_tag="VGD55_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869268.1"
                     /translation="MEVADPDLADSSDSGSLRWRRAVRGGLTGGVGRAVALLLSLAVT
                     PIAINRAGSTTYGLFAALTAFATLLAFSDFGVGNGVIRDLVAARARSGAPGADPEGAN
                     AVDDEGPITATALLVLIVLGLVVTAIGAVAAFTLPWQTLLKAPTADARSLRFAVLAVA
                     VSTGFAVPGALAQKIYLARQRSAQASLWAAAAGAVGSVALFIVVLGNHRLTLMVGAQL
                     GAPALVGIVALWWLARTRTVSFKADQISFAVVKTLVKSGRLFAVLQFVAIINFEVDNL
                     IVARLLGPAKVTQFAVTAGLFAIPLTISAMFFTPLWSAFADAVAVHDVEWVRTAYRRS
                     TRNALVVLIPTAAVLAVVARPAVHIWTRGAVHSPELLVVAFSVWLVVYGFNQPQAMLL
                     NALHAERFQIWAASANLVVNLTLSIVLTQAFGVSGVIWGTEIAQSGVLTATTVYLRRW
                     NANWPTAR"
     gene            complement(5041..5874)
                     /locus_tag="VGD55_02630"
     CDS             complement(5041..5874)
                     /locus_tag="VGD55_02630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869269.1"
                     /translation="MSAVKTVTAITPVRNAASLVARTVSSIVSQSAVTSGRVRLQYLL
                     CDGASSDDTLGEAARAAGEASLETISEPDDGMYDALAKGLRRAEGDLIFYLNAGDVLF
                     PGALDVVVTIAEQTGAKWISGYGAIVNADGVVVSMNLPFRYRRRLLRTSAYGSLLPTL
                     QQESTFWRAELMTNTDLDELATYRLAGDHYLWSTFSATEEPTIVKALLGGFSYHGDHL
                     SRDKAGYRAEIARHAPRVGARDLPTALFDSVAWRAPDRVKKALNRGHLLGYDEASTAW
                     K"
     gene            complement(5871..>6118)
                     /locus_tag="VGD55_02635"
     CDS             complement(5871..>6118)
                     /locus_tag="VGD55_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869270.1"
                     /translation="ARQSTRFVAVNELTAAFRRHGSNLSLTGPTSDLENDEIRCAFGP
                     RSGATRAAGRFSTRVYINARNARWSLTKRVPVRGGGR"
BASE COUNT         1160 a         1921 c         2008 g         1029 t
ORIGIN      
        1 tatctcactc gacggtgccg tcgcggaaat cacccgatgg atcgacgacg gcgagcacca
       61 ctacgtctgc gtcaccaacg tgcacaccgt tatcgaatgc cagcacgacg aagagctgcg
      121 tcgcattcac aacgagtcgg gcctgacgac tcccgacgga atgccgatcg tgtggtgtgc
      181 aaagcgagcc ggcgctgacg gtgtgacgcg cgtgtacggg cccgatttga tgcaggcact
      241 ttcgaagtcg ctcgcgcagt cagggagttc ggtcttcttg tacgggacga cggcgcgcac
      301 cctcgagttg ttgaccgcgc gtctgatctc tgacttcccc ggcctacgca tcgtgggctc
      361 atacgcgcca ccgttccggc cgctgacgcc aactgaggac gccgaaatcg tgcgacggat
      421 caactacagc ggcgcggacg tcgtgtgggt cggccttggt gcagtgaagc aggaatactg
      481 gatggccgaa cacatcgacg tcctgcatgc tcatgctttg atcggtgttg gcgcggcgtt
      541 cgacttccac gccggtgtcg tgaagcaggc accgctgtgg atgcagcgac gtggtcttga
      601 gtggttctac cggttgtgcc gcgaaccgcg tcggctgtgg cggcgctatc tgcgtacgaa
      661 cccagccttc gtgctcggcg tgctccggcg tccgccgcgc ctcagatctt aggttcctgc
      721 gccagcagtg atacgtcagc agccgtagca acgccagcca cctctcgata cagcgcgagg
      781 aatcggcgcg cggtgtcgag ccacgaaaac tccgagacgc gaagcttccc ttgcgttatg
      841 agtgccgcgc gcaactgtgg gtcggtccat agctggctca tcgcgtcagc aatctcttcg
      901 acgtcgtatg gatcgaacac gatcgcggca tcaccaacct ggcgcggcaa tgctgttact
      961 gatgagcacg cgaccgggac gccacgctcg aacgcttccc agacagggaa gctcgccgac
     1021 tcgaagagcg tcggcacgac gacgccacgt gcgtgctggt aaacggctgc gagatcacgc
     1081 tccggcaaga aacccaacac gtgtacgtga tccgatagcc ccaggtcgcg cacgttagca
     1141 gtgaggtcga cgcctgtgcg agctccggtc aagacgaggt gcgcgtcggg atgcgcgtcg
     1201 acgagtatgc gaaatgcttg aagcaggcgc atgtgattct tgtgcggcca ggctgccgcc
     1261 ggatacagca gatagctggc gggcaggcca gctgggcttt gggtcgacgc ggtgacggtc
     1321 gcaaccggtg cgagcggaat cacgacgacc tttgctggct cgatgccaag cttggcgaca
     1381 acgtcgtcct tgacccacga cgtacctacc gcgactctgg ccgccgctcg gcacaacggg
     1441 ccatagaaag tttcgcgccg agtcagcgtc tccttagaga agaactccgg catgtggaga
     1501 tgctgaaggt cgtgcgggtg atagatgaac ggcgcagcga ccaggccaac ctcctgatga
     1561 ggaaggtgca cgacgtcggg cttgagtgcg tcgagctggt gatcgtgcgg aagccgggtg
     1621 tgaactccct tgatggagtt gcgcagacgc cgcgccggtt cggcgagcgg accgagcgag
     1681 cggcgcagct gcggcgacgg cttgtgcggc ggttgcactt cgatgagctg gatcgactca
     1741 ccgatgagcc tggcaagcca ctcggtatga ccggtgtagg caacgaagga gatgtcgacg
     1801 tcagggtgct cgatagctga caagccatcg gctaggccga ccaggacggt ctcgacacca
     1861 ccacttgcgc cagtgaagag acgcgcatcg acggcaactc gcacagagtt ggtcatgcgc
     1921 cggtcatcgc gctggtcatc gtgctgccga ggcgatcgcg gcgcacacct tgtctacgtc
     1981 gacgtcagtc atctgggaga acgtcggcag attgatgccg tcgcgcgcca accgcatggt
     2041 gaccgaaaga tttgaggtgg acgccgaagc atacggtggc agcgtgtgca caggtatgaa
     2101 gaatggccgc gtctcgatct cgacctccgc caatgctgcg gccaagtcgt ctcgcgtgcg
     2161 gccgaactct tcgccgacga gtactgagta cagccagagc gctcgcgtct ggccggcgac
     2221 gtcggcttga agctccaacc cgcgtatgtc gcgcaggcct tctgtgtaac gcgcgaaaat
     2281 ggcggtgcgt cgctcgatca gttcgtggcg acgctccatt tgtgcgcaca gcaacgcggc
     2341 cgcgatgttg gtcagacggt agttgtagcc gatgaccgga aagaagtatc gacggttcag
     2401 atccatgccc tggccacgga acaacttcat gcgcgccgtg aggtcgtcgt cgtcggacgt
     2461 cagagcgccg ccctcgccgc tggtcaggat cttgttgccg tagaacgaga aggtcgcaac
     2521 atcgccgagg ctgcctgtcg gtcgaccacc gtaggacgca cacggtgctt ccgcggcgtc
     2581 ctcgacgaca tgcagcgcgt gcgcgcgcgc aatcgaccgc agcgcatcca tgtctgcggg
     2641 ctgcccatag aggtgcaccg ccatgatggc ccttgtttgt ggcgtgaccg ctaaagctac
     2701 tgccgcgggg tcaatgcacc acgtgaccgg atcgacatcg acgaacaccg gtcgggcacc
     2761 aacgtacgtg acggcgttca ccgacgcgat gtaggtcagc gacgggacga tcacctcgtc
     2821 gccgggccca atgccaaggc caaggagagc tagatgcaaa gcaacggtcc cgttcgagac
     2881 tgctagcgca tgtcgcgtcc cgcacatctc ggcgaactcc gactcgaacc gctcgaggta
     2941 cggcccggcc gaggcaatcc atgatgtgtg gatcgcgtcg gtcacatacg ccgtctcacg
     3001 tggcgataga tccggccgcg ccagggggat cgcggtcact gctcgggacc gcgattcagc
     3061 gggcgcgctg ggataccgac gacggttgta cccgctgcga cgtccttcgt gacgaccgcg
     3121 cccgctccca cgacggcacc cgcctcaacg accaggtgtt cgagcacgac ggcgcctgct
     3181 cccagtaggc agccacggcc gatgtggcag tcgcccgaaa cggctgcacc tgggttcaca
     3241 acggttccgg ccccgatgcg gcagtcatgg ccgatggtga cattgaagtt gatcagcgcc
     3301 gcttcttcga tcacgacatt cgtggtgatg acagatcctg aactgatgca ggcgccccgg
     3361 ccaacggttg aggtcgggcc gacatcggct cggggatgga tcagtatcgg ccactcgagg
     3421 ccgggtcgat cgcagtactt ctcccaaaga tgcagccgca aagtcggcgt gccgaccgcg
     3481 agcgcggcct caccaccgtc ggcgagagca cggtcctcat ccccgggtcc gagaaacacc
     3541 agctcgcgcc agcgctcgcc aagaggatcc attgcgcgcg cgatgagcgc gacttcgggt
     3601 gcgtggccac cgttgccgag aaggaacagg ggctggctca tggctggctc gctgacgcca
     3661 tttgcggact gtatcagcgc gccgttggcc agttggcgtt ccaacgccga aggtagacag
     3721 tcgtcgccgt caggacgccg gactgggcaa tctcagtgcc ccagataaca cccgacacgc
     3781 cgaaggcctg agtcaacacg atcgacaacg tcaggttgac gacgaggttg gctgatgctg
     3841 cccagatctg gaaccgttcg gcgtgcagcg cgttcagcag catcgcctga ggctggttga
     3901 atccgtagac gacgagccag accgagaacg cgacaacgag cagttccggt gaatggacag
     3961 cgccgcgcgt ccagatatgt accgcgggtc gtgccacgac ggccagcacc gcggcggtcg
     4021 gtatcagcac gaccaaggca ttgcgggtcg atcgccggta ggcggtccgc acccattcga
     4081 cgtcgtgaac tgcaacggcg tcggcgaacg cactccatag cggcgtgaag aacatcgctg
     4141 agatggtgag agggatcgca aacaggccgg cggtaaccgc gaactgggtc acctttgccg
     4201 ggccgaggag tcgggcgact atcaggttgt cgacctcaaa gttgatgatg gcgacgaatt
     4261 gcaggacggc gaagagccgg ccgctcttga cgagcgtctt caccacggcg aacgagatct
     4321 ggtcggcctt gaaagacacc gtgcgcgtgc gggcgagcca ccagagggcc acgattccca
     4381 ccagcgcggg ggcgccgagc tgcgcgccaa ccattaacgt cagtcggtgg ttgcccaaga
     4441 ccacgatgaa cagggcgacg gatccgacgg ccccggcggc agcagcccac aggctggctt
     4501 gcgcagagcg ctggcgggcg aggtagatct tctgagccag cgcgcccggg accgcgaacc
     4561 ccgtcgacac cgccaccgcg agcactgcga agcggaggga cctcgcgtcg gcggtaggcg
     4621 ccttcagcag ggtctgccag gggagggtaa aggcagccac cgcgccgatc gcggtcacga
     4681 caaggccaag gacgatgagc acgaggagcg cagttgccgt aatcggtccc tcgtcatcga
     4741 cggcgtttgc gccctcaggg tcggcgccag gtgctcctga ccgcgcccgc gccgcgacaa
     4801 gatcccggat cacaccgttg ccgacgccga aatcggaaaa ggcgagaagc gtcgcgaacg
     4861 cagtcagtgc ggcgaacagc ccgtaggtcg tcgagcccgc gcgattgatg gcgatgggag
     4921 tgacggccaa cgagagcagc agtgcgactg cgcggccgac accgccggtg agaccgcctc
     4981 gaacagcgcg acgccatcga aggcttccgc tgtcactcga atctgcaaga tcaggatcgg
     5041 ctacttccac gccgtgctcg cttcgtcgta cccgagaagg tggccacgat tgagcgcctt
     5101 cttgacgcgg tcaggcgcac gccacgcgac gctgtcgaat agggcggtgg ggaggtcgcg
     5161 cgcgccgacg cgtggcgcat gtcgggctat ctctgcgcgg tagccggcct tgtcccgcga
     5221 caagtgatcc ccgtggtagc tgaacccgcc gagcagcgcc ttcacgatgg tcggctcctc
     5281 ggtagccgag aacgtgctcc acaggtagtg gtcaccggca aggcggtacg tagccagctc
     5341 gtccaggtca gtgttggtca taagttccgc gcgccaaaac gtcgactcct gctgcaacgt
     5401 cggaagcagt gagccgtaag cactggttcg caagagtcgg cgacggtaac ggaatggcag
     5461 gttcatgctg acgaccacac cgtcagcgtt cacgattgcg ccgtacccgc tgatccactt
     5521 tgcaccggtc tgctcggcga tggtgacgac aacgtcgagt gcgcccggga acagcacgtc
     5581 gcccgcgttg aggtagaaga tcaggtctcc ctcggcccgc cgcagtcctt tcgccaatgc
     5641 gtcgtacatg ccgtcgtcgg gttcggaaat cgtctcgagg ctagcctcgc cggcagcacg
     5701 cgccgcttca cctaatgtgt cgtcgctcga tgctccgtcg cacagcaggt attgcaggcg
     5761 cactcgaccc gacgtgaccg cgctttggct cacgatcgat gacacagttc gggccaccag
     5821 tgacgcagcg ttgcgcacgg gagtgatagc ggtcacggtc ttcaccgcac tcatcggccg
     5881 ccccctcgca ccggcacccg cttggtcagt gaccaacgag cattgcgtgc gttgatgtag
     5941 acgcgcgtgc tgaatcggcc agcggctctg gtcgccccag aacgaggccc gaacgcgcac
     6001 ctgatctcgt cattctcgag gtcggacgtc ggcccggtca gactcaagtt ggaaccgtgc
     6061 cgcctgaagg cggcggtgag ttcgttgacg gcgacgaagc gggtgctttg acgcgccc
//
LOCUS       DASWVF010000153         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_4127, whole genome shotgun sequence.
ACCESSION   DASWVF010000153 DASWVF010000000
VERSION     DASWVF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGD55_02640"
     CDS             complement(<1..348)
                     /locus_tag="VGD55_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869271.1"
                     /translation="MTTSTQVPVGTPGAARRGHHPGLALAVIATSQLMVVLDATIVNV
                     ALPTIRGALHFSYTNLTWELNAYTLTFGGLLLLGGRAGDILGRRRVFIFGIALFTFAS
                     FLGGLATSSWWLLA"
     gene            466..1131
                     /locus_tag="VGD55_02645"
     CDS             466..1131
                     /locus_tag="VGD55_02645"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HEY0869272.1"
                     /translation="MPAARLRSSTTLATAADKNPGVPLFRCRRRGLALESAIYDAVLQ
                     QLSTVGFGAMTMEGVAAAARTGKAAIYRRWPSKEELVADTLDNVLPSLDLPLDTGNVR
                     SDLAAIFSVMLDMMASPAGGAMQALMGELGRDHDFVTTLHERVLAPRKALMTAILLRG
                     VARGDVRADAVKPVVVDAGPALLMHRLLTFGPPVDPAYMEAVLDDVVMPLISPLTTSV
                     ASS"
     gene            complement(1128..>1566)
                     /locus_tag="VGD55_02650"
     CDS             complement(1128..>1566)
                     /locus_tag="VGD55_02650"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0869273.1"
                     /translation="FLPAANDYLLRQGWSEGFATAVHLVVDLSTGAYIVESAGHPPAV
                     HFVKGSGTWRLIDSEGTVLGVLAEASYTSAHGVLGSGDAMLLFTDGVVETAEREIAVG
                     IDKLIGEAERLVTQGFRDGAKQLVDRVAPDGSDDRALVLIWRD"
BASE COUNT          315 a          498 c          483 g          270 t
ORIGIN      
        1 ggcgagcagc caccaagacg acgttgcgag gccaccgagg aacgaggcga aggtgaacaa
       61 cgcgataccg aagatgaaga cgcgtcgacg cccgaggatg tcgccggcgc ggccaccaag
      121 aaggagcaac ccaccgaaag tcagcgtgta ggcgttcaac tcccaggtga gattcgtgta
      181 cgagaagtgc agcgcgcccc tgatggtcgg taacgcgacg ttgacgatcg tggcgtcgag
      241 caccaccatc agctggctgg tcgcgataac ggcgagcgcg aggcccgggt ggtggccgcg
      301 tcgggcggca cccggagtgc ccacgggtac ctgcgtcgag gtcgtcatga atctctcgtt
      361 tccatgggag gaacgctgaa ggaacgctaa cgttcactaa ttacggacgc tacagtgcac
      421 gagatagaga acgcaaccgt tccttcgcct ggtgaggagg cagccgtgcc agctgcacgt
      481 ttgcggtcat cgaccacgct agcaacggcc gccgacaaga acccgggcgt gcccttgttc
      541 cgatgtcgcc gccgaggtct ggctctcgaa tctgccatct acgacgccgt ccttcagcag
      601 ctgagcaccg tcgggttcgg tgcgatgacg atggagggtg tcgctgccgc tgcgcgcacc
      661 ggcaaagcag cgatctaccg gcgttggccc tcaaaagaag agctggtcgc cgacaccctc
      721 gacaacgtgc tgccctcgtt ggatcttccg ctcgacaccg ggaacgttcg cagcgacctc
      781 gcggcgatct tcagtgtgat gctcgacatg atggccagcc ccgccggtgg tgcgatgcaa
      841 gcactgatgg gtgagctcgg gcgtgaccac gacttcgtca ccacattgca cgaaagagtg
      901 ttggcaccgc ggaaggcatt gatgacagcg atcttgttgc gcggagtcgc ccgcggcgac
      961 gtccgcgccg acgccgtcaa accagtcgtt gtcgatgctg gtcccgcgct gctgatgcac
     1021 cgactactga cgttcggccc acccgtcgac ccggcgtaca tggaagcagt actcgacgat
     1081 gtcgtgatgc cgttgatcag cccgctgacg acgtcggttg catcgagtta gtcgcgccag
     1141 atcagcacga gcgcacggtc gtcgctaccg tcgggcgcca cccggtcgac caattgcttc
     1201 gcaccatcgc gaaatccctg tgttaccagg cgttctgcct caccaatgag cttgtcgatg
     1261 ccgacggcta tttcgcgttc tgccgtctcg accacgccgt cggtgaacag caacatcgcg
     1321 tcgcccgaac cgagcacacc gtgcgccgag gtatagctcg cttccgccag cacaccgagc
     1381 acggtgcctt cactgtcgat gaggcgccac gtgccgctgc ctttcacaaa atgcacggca
     1441 ggcggatgcc cagcggactc gacgatgtag gcaccagtgc tcaaatcaac aacaagatgg
     1501 acggcggttg cgaacccttc gctccacccc tgacgtagca gatagtcatt ggctgcaggc
     1561 aaaaac
//
LOCUS       DASWVF010000154         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_284371, whole genome shotgun sequence.
ACCESSION   DASWVF010000154 DASWVF010000000
VERSION     DASWVF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284371"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VGD55_02655"
     CDS             complement(<1..603)
                     /locus_tag="VGD55_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0869274.1"
                     /translation="MRLTVVGCSGSHPGPESSCSCYLVEHDGFRLLLDLGNGSLGPLQ
                     RFINPNDIDAIYLSHLHGDHWLDLVPLAHVRRHPRDAEVRVLPVMAPVAERGRIAGAF
                     GVPASELSDIYAFEDPADGAIGPFDVKLIRTQHPVETHAIRLSAGGKTLVYTADTGPF
                     DSLAGFALGADLLLAEAGFADGMPPKMHLRGMEGGQLAADA"
     gene            complement(613..1476)
                     /gene="murI"
                     /locus_tag="VGD55_02660"
     CDS             complement(613..1476)
                     /gene="murI"
                     /locus_tag="VGD55_02660"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0869275.1"
                     /translation="MADIMPGRADVGRLAAVTNVADVTADDSPIGVFDSGVGGLTVAR
                     AMLDLLPHEPLYYVADTANFPYGPKSIAEVRECALKVLDEMVDHGVKMLVIACNSASS
                     AMLRDARERYDVPVVEVIQPAVRKAVTATRNGRVGVIGTHATITSMAYEDAFAAAPKI
                     DLTTAACPRFVEFVEAGITMSDELLSCAKEYLAPIVERGCDTLVLGCTHYPFLAGALS
                     LVVGDDVMLVNSADETALDVYRTLIRHRLSRSESAAPPRHTFVATGDPEAFFELGRRF
                     LGPEILAVEGV"
BASE COUNT          287 a          501 c          493 g          257 t
ORIGIN      
        1 ggcgtcggca gccaactggc caccctccat gccgcgcagg tgcatcttcg gcggcatgcc
       61 gtcggcaaag cctgcctcgg ccaacaagag gtcggcgccc agcgcgaagc cggcgagtga
      121 gtcgaacggg ccggtgtctg cggtgtagac gagcgttttg ccccctgcgc taaggcgaat
      181 cgcgtgagtc tcgactgggt gctgggtgcg aatgagcttg acgtcgaatg gcccgatcgc
      241 accgtctgcg ggatcctcga acgcatagat gtcgctgagc tctgatgccg gaactccgaa
      301 cgcgccagca atgcgcccac gttcggcgac cggcgccatg accggcagca cccgcacctc
      361 ggcgtcgcgc ggatgccgtc tcacgtgggc cagcggaacc agatcgagcc agtgatcgcc
      421 atgcaggtga ctcaggtaga tcgcatcgat gtcgttcggg ttgatgaacc tttgcaacgg
      481 accaagcgac ccgttgccca aatcaagcaa cagcctgaaa ccgtcgtgct cgacgaggta
      541 gcacgagcaa gaagattcgg gcccaggatg gcttcccgag cagcccacca cagtgagtcg
      601 catgacggtt cgtcagaccc cttcgacggc caagatttca gggccaagga aacggcgtcc
      661 gagttcaaag aatgcctcag gatcgccggt cgcgacgaac gtgtgtcgcg gcggtgctgc
      721 cgactccgac cgcgacaacc tgtggcggat caacgtgcgg tagacgtcga gcgcggtctc
      781 gtcggcgctg ttgacgagca tcacgtcgtc gccgaccacg aggctcaacg ctccggccag
      841 gaacgggtag tgcgtgcagc cgagcacgag agtgtcgcag ccacgctcga cgatcggcgc
      901 caggtactcc tttgcacagc tgagcaactc atcgctcatg gtgatgccgg cttcgacgaa
      961 ctcgacgaat cgcgggcacg cagcggttgt caggtcgatc tttggcgcag cggcgaacgc
     1021 atcttcgtac gccatgctgg tgatcgtggc gtgggtgccg atgacgccaa cgcgcccgtt
     1081 ccgggtcgcc gtcacggcct tgcgcaccgc gggttgaatg acctcgacga ccggtacgtc
     1141 gtagcgttcg cgtgcatcgc gcaacatggc cgacgacgcc gagttgcatg cgatcaccaa
     1201 catcttgacg ccgtggtcga ccatctcgtc gagcaccttc aacgcacact ctcgaacctc
     1261 ggcgatcgac ttcggaccgt agggaaagtt cgcggtgtct gcgacgtagt acaacggttc
     1321 gtggggcagc agatcgagca tcgcgcgggc gacggtcaga ccgccgacgc cactgtcgaa
     1381 gacgccgatc ggcgaatcgt cggccgtcac gtccgccaca tttgtcacgg cggccagcct
     1441 accgacgtcc gcccggccag gcatgatgtc ggccatgtcg cgaaccctcg ttctgatcgg
     1501 cgctcccacc agtgcgggca gttacgcccc cggccagg
//
LOCUS       DASWVF010000155         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_309730, whole genome shotgun sequence.
ACCESSION   DASWVF010000155 DASWVF010000000
VERSION     DASWVF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309730"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VGD55_02665"
     CDS             complement(<1..814)
                     /locus_tag="VGD55_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY0869276.1"
                     /translation="MTWATAEILTMLRGMTHQLTPSLAALAAAQGGPFTTAQAMEAGY
                     DRREILRFLRSGAWIRLRRGVYADRTLVPDDENGRHLIQLRAVLLCLVAPVAASHVTG
                     VALQGIALLDPDFSLIHITREGAGSSRTEAGIRHHDAALPPGQLTKVDGVVGTSAART
                     ILDYARSATFEVAVVAAESALNKNLTTLAELREVLDYCVDWPGARQAGRVVSFASPHS
                     ESPGETMGRIAFDHFGLPQPEQQIELYDAMGFVARVDYYWKQHRTVAEFDGRL"
     gene            complement(942..1964)
                     /gene="fbaA"
                     /locus_tag="VGD55_02670"
     CDS             complement(942..1964)
                     /gene="fbaA"
                     /locus_tag="VGD55_02670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY0869277.1"
                     /translation="MPIATPEVYADMIDRAKAGKFAYPAINVTSSSSINAAIQGFADA
                     ESDGIIQFSWGGAEFASGQRVKDMVIGAVALAEFAHVVAAKYPVNIALHTDHCPPNKL
                     EGYMRPLIEISKGRVAAGQNPLFGSHMWDGSAVSLEENLKIARELLAGCAEAHIIMEL
                     EIGVVGGEEDGVSHEINEKLYTTPEDALRTAEVLGLGENGRYLLAATFGNVHGVYKPG
                     GVKLRPSVLKEIQDEVGAKYGRVEPFDLVFHGGSGSLLEEIRETLDYGVVKMNVDTDT
                     QYAYTRPVVDHVFKNYDGVLKIDGEVGNKKAYDPRAWSKSAENGMAARVVEACTDLRS
                     TGTKAT"
     gene            2114..2764
                     /locus_tag="VGD55_02675"
     CDS             2114..2764
                     /locus_tag="VGD55_02675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0869278.1"
                     /translation="MISHFVAASTHASVNILDAGSLIKTFGLAGIFIVLFIQTGLLIG
                     FFLPGDTLLALAGAFAALPSSDSRHLPLVALLIGCPVAAIIGGQVGFVIGQRGGHVIA
                     KPGSSFEHSIRRIEPLLARFGQGWVVLICRFVPVLRTFINPAAGILEMPTRTFTLWNV
                     VGGIAWPVGILLIGYYAGVKLHIEKYALPVVAVLVLASVVSVLLEMRRNKHRQVSG"
BASE COUNT          459 a          938 c          957 g          582 t
ORIGIN      
        1 tcagccggcc gtcaaattcg gcgacagtgc gatgctgttt ccagtagtag tcgactctcg
       61 cgacgaatcc catcgcgtcg tacagctcta tctgttgttc tggctgcggt agaccgaagt
      121 ggtcgaacgc gatgcggccc atggtctcgc caggagactc cgagtgcggc gaggcgaacg
      181 acacgactcg ccccgcctgt cgcgccccgg gccaatcgac gcagtagtcc agcacctctc
      241 gtagttcggc cagcgtcgtc aggttcttgt tcagtgcgga ctcggcagcg acaacggcca
      301 cttcgaaggt tgcactgcgg gcgtagtcga ggatggtccg ggccgcactt gttccgacga
      361 cgccgtcgac cttggtcaat tggcccggcg gaagcgccgc gtcgtggtgt cgaattcctg
      421 cctccgttct cgaggatcct gcgccttcgc gggtgatgtg gatgagcgag aagtcaggat
      481 cgaggagcgc gattccttgt agcgcgacgc ctgtgacgtg gctcgctgca accggggcga
      541 cgagacaaag aagaacggcg cgtaactgga tcagatgacg gccgttctcg tcgtccggaa
      601 cgagcgtgcg atcggcgtat acgccccggc gcagtcgaat ccacgctccc gatcggagga
      661 accggaggat ttcgcggcgg tcatagccag cctccatagc ctgggccgtc gtgaacggac
      721 cgccttgtgc cgcggctagg gcagccaggc tcggagtcag ttggtgtgtc atgccgcgaa
      781 gcatggtgag gatttcggcg gtggcccacg tcatccgcag gctttctcgt gtgggcttcc
      841 gctatccaca ggccgaggac aggcttacgt ccccgaggac cggctcgtaa ccctgtcctc
      901 gggggccgtg accacgttta cgtgatcgac gcgcggcagg gctaggtggc tttggtgccg
      961 gtcgagcgga ggtcggtgca cgcttcgacg acgcgcgcgg ccatcccgtt ctcggccgac
     1021 ttcgaccacg cccgcgggtc gtaggccttc ttgttgccga cttccccgtc gatcttcagc
     1081 acgccgtcgt agttcttgaa gacgtggtcg acgaccggcc tcgtgtaggc gtactgcgtg
     1141 tcggtgtcga cgttcatctt caccacgccg tagtcgagcg tctcgcggat ctcctcaagc
     1201 aacgacccgg aaccgccgtg gaacaccagg tcgaaaggct cgacgcggcc gtacttcgcg
     1261 ccgacttcgt cctggatttc cttcagcacg gagggccgca gcttgacgcc gcccggcttg
     1321 tagacgccgt gcacgttgcc gaacgtcgcc gccaacaagt agcggccgtt ctcaccgagc
     1381 ccgagcacct cagccgtgcg cagcgcgtct tcgggggtcg tgtagagctt ctcgttgatc
     1441 tcgtgcgaga cgccgtcttc ttcgccgccc acaacgccga tctcgagctc catgatgatg
     1501 tgcgcctcag cgcagccggc caggagctcg cgtgcgatct tcagattctc ctcgagcgac
     1561 actgccgagc cgtcccacat gtgcgagccg aacagcgggt tctggcctgc cgccacccgc
     1621 cccttcgaga tctcgatcag tggccgcatg tagccctcga gcttgttcgg cgggcagtgg
     1681 tcggtgtgca gcgcgatgtt caccgggtac ttcgcggcca cgacatgggc gaactcggca
     1741 agggcgacgg cgccgatcac catgtccttc acccgctggc ctgacgcgaa ctcggccccg
     1801 ccccacgaga actggatgat gccgtcactt tcggcgtcgg cgaagccctg gatcgcggcg
     1861 ttgatcgacg acgacgacgt gacgttgatc gccggatagg cgaacttgcc ggccttcgcc
     1921 cgatcgatca tgtcggcgta gacctcgggg gtggcgatcg gcatgtggtg ctcctgacag
     1981 gttcaaaacg acgggggcgg gacggcgcgg gcaaccgata tcggtcgctt cagaagtatc
     2041 ccgcacaggg aaagcccggc tgcgcccacc tgcctcgggg tgggcggcag ttcggcttgg
     2101 atagcctcga cccatgatca gccacttcgt cgcggccagc acgcacgcgt cggtcaacat
     2161 cctcgacgct gggtccctta tcaagacctt cggcctggca ggcatcttca tcgtcttgtt
     2221 catccagacc ggcttgctaa tcggtttctt cttacccggc gacacattgc tcgccctcgc
     2281 cggcgcgttc gcggcgctgc cgtcgtccga ctcgcggcat ctcccgttag tggcgctgct
     2341 gatcggttgc ccagtggcgg cgatcatcgg gggtcaggtc ggattcgtga tcggccagcg
     2401 cggcgggcat gtcatagcca agccggggtc gtcgtttgag cactcgatcc ggcgaatcga
     2461 gccactgttg gcgcggtttg gccagggctg ggttgtgctg atctgccgat tcgttcccgt
     2521 cttgcgtacc ttcatcaacc cggcggcggg gatactggag atgccgaccc ggacgttcac
     2581 gctctggaat gtggtcggcg ggatcgcgtg gccggtcgga atcctgttga tcggctacta
     2641 cgcaggcgtg aaactgcaca tcgaaaagta cgcgctgccc gtggttgccg tgctcgtgct
     2701 ggcatccgtg gtgtccgtgt tgctcgagat gcgtcgcaac aagcaccggc aggtctccgg
     2761 ctgatccgtt cggtttggct gcgaggtcag accggtacaa gccaggtagt ccgtacccga
     2821 acatcgtgga ggcctgaccg ggtgagcgac gccgacctag ccatcgtgac gcgcgacctg
     2881 accaagacgt ttacggttgc gcgtcgccac gttggccgct ttgctgggtt gcgcgc
//
LOCUS       DASWVF010000156         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_411891, whole genome shotgun sequence.
ACCESSION   DASWVF010000156 DASWVF010000000
VERSION     DASWVF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411891"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VGD55_02680"
     CDS             complement(<1..307)
                     /locus_tag="VGD55_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869279.1"
                     /translation="MTDTTSTGVGSFVQPDASATAVQRRRTHRPLLRKRTGAGERKLT
                     PWLFMLVPLAFLLVFSYIPVVNLFRYSLTSWDGFSHNRPYVGLKNYIDLFTRPELFEV
                     "
     gene            complement(421..1596)
                     /locus_tag="VGD55_02685"
     CDS             complement(421..1596)
                     /locus_tag="VGD55_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0869280.1"
                     /translation="MTNRTDLTADGTMAKYAADFNKIYPNVKVTFQGITNYEGDVKTR
                     MNTSNYGDVLLIPSALPISEYPTFFEPLGSATDLSAKYNFIANGTVNGQVYGIAQNGN
                     ANGFVYNKTVWAQAGITTWPTTPDQYISDLQAIKAKTSAIPYYTNYKDGWPLSSWEGA
                     VGSPSCNAQANDNLATETAPWSSGNDLFQIDSLLFNIVKDKLSEPDPTTTNWENSKGL
                     LATGKISAMWLGSWAVSQMQAAAKTAGQDPSEIGFMPWPSATGGHLCSVIGPDYLQGI
                     NIHSSHKDAARAWIDWFTDQSTYAVDNGDVPTLKSATQPPALAAYAAAGVTLVTLTQT
                     NSALVNKIDNSAEIGLNSQTYRQHLVDIARGAAGGSLQSDFDSLNSKWGAAQKTGGA"
     gene            1601..1888
                     /locus_tag="VGD55_02690"
     CDS             1601..1888
                     /locus_tag="VGD55_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869281.1"
                     /translation="MMSPDTVVGIVDDAADDAAGALDEGAEAAELVAAALGAALDADG
                     VDVAVVPLLLLQAATSEPTTSNTPNEVFFTLTISISFRFGRRRPAGTATRD"
BASE COUNT          355 a          658 c          671 g          427 t
ORIGIN      
        1 acacctcgaa gagttcgggc ctggtgaaca gatcgatgta gttcttcaga ccgacgtacg
       61 gccggttgtg gctgaaaccg tcccaactcg tcaggctgta acggaagagg ttgacgaccg
      121 ggatgtagct gaacaccaac agaaaagcca gcggcacgag catgaacagc cacggcgtca
      181 gcttccgctc acccgcgccg gtccgcttac gcaacagtgg ccggtgggtg cgacgacgct
      241 gtacagccgt cgcactggcg tccggttgca cgaaggagcc gacgccggtg ctggtggtgt
      301 cagtcatgcg cgcccctttc ctgctctaga acgtccacga cacgggtgcg tggacggcgg
      361 gtgcttgcgc gttcccgccg cccacgacgc tgcctcctct tgaagccgtg cgttcgctag
      421 ttacgcgccg ccggtcttct gcgcggctcc ccacttgctg ttcaagctgt cgaagtcgga
      481 ctggaggcta ccgccggccg caccacgtgc gatgtcgacc aagtgctgcc ggtaggtctg
      541 gctgttcaac ccgatctcgg cggagttgtc gatcttgttc accaacgccg agttggtctg
      601 cgtgagcgtg accagcgtga cgccggccgc cgcgtaagcc gcgagagcag ggggctgggt
      661 ggcgctcttc aaggtcggca cgtcgccgtt gtcgaccgcg taggtcgact gatccgtgaa
      721 ccaatcgatc catgcacgcg cagcgtcctt gtggctggag tggatgttga tcccctgcag
      781 gtagtcagga ccgatcacag agcacaggtg accacccgtc gccgacggcc atggcataaa
      841 cccgatctct gatgggtcct gacctgccgt ctttgctgcg gcttgcatct gcgaaacggc
      901 ccacgagccg agccacatgg ccgagatctt cccggtggcc aacagcccct tcgagttctc
      961 ccagttcgtg gtcgtcgggt cgggctcgct caacttgtcc ttgacgatgt tgaacagaag
     1021 cgagtcgatc tggaagaggt cgttgcccga gctccacgga gcagtctcgg tcgcaaggtt
     1081 gtcgttcgcc tgtgcgttgc agctcggcga tccgacggcg ccttcccacg agctcagcgg
     1141 ccagccgtcc ttgtagttcg tgtagtacgg gatcgccgac gtcttggcct tgatcgcctg
     1201 caagtcagag atgtactggt caggcgtcgt cggccaggtc gtgatgccgg cctgcgccca
     1261 gaccgtcttg ttgtagacga acccgttcgc gttgccgttc tgcgcgatgc cgtacacctg
     1321 cccgttgacc gtgccgttcg cgatgaagtt gtacttggct gagagatcgg tcgcggatcc
     1381 gagcggctcg aagaatgtcg ggtactccga aatcggaagt gcgctcggga tcagcaggac
     1441 gtcgccgtag ttcgacgtgt tcatgcgggt cttgacgtca ccctcgtagt tcgtgatgcc
     1501 ctggaacgtc accttcacgt tcgggtagat cttgttgaag tctgccgcgt acttcgccat
     1561 cgtcccgtcg gctgtcaggt cggtgcggtt cgtcaagacc gtgatgtcac cggacacggt
     1621 cgtcggtatc gtcgatgatg ccgcggacga tgccgctggc gcgctcgacg aaggcgcgga
     1681 ggccgccgaa ctggttgccg cagcgcttgg cgcggcactc gacgctgacg gggtcgacgt
     1741 cgcagttgtc cccttactgc tgctacaagc cgccacgagc gagccgacga cgagcaacac
     1801 ccccaacgag gtcttcttca cattgaccat ctccatctca ttccgattcg gccgccgacg
     1861 tcccgcgggc accgcgacta gggattaaac ggttcagtaa ctgatcgagc gccacggtaa
     1921 ggagcctgtg gatcgcggtc aaggtccagc acgctgtcgg tgccgacttg atacgaaact
     1981 gttacgcgcg gattaagcct tagtggcagc gaatttctgc cagcgagcgg gaacgcaccc
     2041 gctggcacgc tcgaatcgcg cctgcgggtt gcaagttccg gtgcgcgacg cgcgccgccg
     2101 ctatgtccgt g
//
LOCUS       DASWVF010000157         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_106222, whole genome shotgun sequence.
ACCESSION   DASWVF010000157 DASWVF010000000
VERSION     DASWVF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106222"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..461
                     /locus_tag="VGD55_02695"
     CDS             102..461
                     /locus_tag="VGD55_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869282.1"
                     /translation="MRWVLIVDDDPDLRETLIDALLLEELNVHAVENGQVALDWLRAH
                     PELDWMVLLDLMMPVMDGRSFLEARARDPVIAAIPVVILTAGGDCRELKLRHDIAGCL
                     HKTVGLQQLLAAVRAVG"
     gene            561..>2375
                     /locus_tag="VGD55_02700"
     CDS             561..>2375
                     /locus_tag="VGD55_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0869283.1"
                     /translation="MGNLVWINTLLAGFFGCAAIHYALYWGFSRRERVFLVFCLQCVA
                     YTVFCLAISTYFEATTIPAAQATLNRFVTIGVLLHVLLLEFFATLGARRDRTFRAVVA
                     GGLVFLALLNHWAPLRGTVLELRTMPLPGGGTGLLAIRTPPGASLALLYVLVFAIDAY
                     GFLVAAKIWRRDRVGGIFVALGTAVYLCGATIGMLVDFAHVRVPYAGALPHAFFVVCV
                     ALHLSRTYAARGARVVATDAQFEAVFEHSPIGKALLGTDGQFLRVNRAFCRIVGATGE
                     ELCARRLYDILRGDDAGFIAVGAERLLRGEVESYACETGLLRSGADLLWALLGLSPVP
                     DAHGQPVQMIAQLQDITEVRAYRERLEDLVARRTRELHHAKDEAERASQAKSWFLTHM
                     SHEIRSPLQVMVLNAATLEGDRSLTPEQVSCIESIQSSGEHLATLINNVLDMSTVESG
                     RLALVETRFDLTAILDETAQMFAAQAAANGTSLRVEVTPGLPASLLGDGGKVKQILIN
                     LVSNAIKFTHRGSVRIAATCSPAAHPTPLVEIVVADTGMGIGNRNLARIFQPFEQLEA
                     GANTGGTGLGLTISLAYARLMGGDITVESRPGAGSRFKL"
BASE COUNT          371 a          790 c          789 g          425 t
ORIGIN      
        1 cacataccgg cggcggatcc gatgcgcctg gaagcggcgg agccggcgcc acagacgctt
       61 cagccctgac ggcggggtac ggggagacga gggcgagcac cgtgcgttgg gtcctgatcg
      121 tcgacgacga ccccgatctg cgtgagacgc tgatcgacgc cctgttgctg gaagagctga
      181 acgtgcacgc cgtcgaaaac ggccaggtcg ccctggactg gctgcgggct caccccgagc
      241 tcgattggat ggtgctgctg gacctcatga tgcccgtcat ggacggccgt tcgttcctgg
      301 aggcgcgggc ccgcgacccg gtcatcgccg ccatccccgt cgtcatcctg accgcgggcg
      361 gcgactgccg cgaactgaag ctacggcacg acatcgccgg ctgcctgcac aagacggtcg
      421 gccttcagca gctcctggcc gcagttcgcg ccgtcggctg acgtcagccc cccgacgaat
      481 tccggtcgcg agcccttttc atccgcaaat cacggtcggt tgataccggc cggcggaggc
      541 ggttaactgc cgcaaccgcg atgggaaacc tcgtttggat caacacgctg ctagccgggt
      601 tcttcggctg cgcggcgatc cactacgcgc tctactgggg cttctctcgt cgcgagcgtg
      661 tgttcctggt tttctgcctg cagtgcgtcg cgtacaccgt cttctgtctg gcgatcagca
      721 cctatttcga ggcgacgacg atccccgccg ctcaggccac gctgaacaga ttcgtcacga
      781 tcggggtgct cctccacgtc ctgctcctgg agttctttgc cacgctcggc gcgcgccgag
      841 accgaacctt tcgcgcggtt gtcgctggtg gactggtctt cttggccctc ctgaaccact
      901 gggcgccact gcgtggaacg gtcctcgaat tgcgaacgat gccgctgcct ggcgggggca
      961 ccggcctgct ggcgatccgc acgccacccg gcgcgtcgct cgcgctcctt tacgtattgg
     1021 tgttcgcgat cgacgcatac ggattcttgg tggccgccaa gatctggcgg cgcgaccggg
     1081 tgggtgggat cttcgtcgcg ctcggtaccg cggtgtacct gtgcggagcc acgatcggaa
     1141 tgctcgtcga cttcgcgcac gtccgggtgc cgtacgccgg cgcgctgccg cacgcgttct
     1201 tcgtcgtgtg cgtcgcgctt cacctgtcgc gcacgtacgc cgcgcgcggt gctcgggtgg
     1261 tggccacgga cgcgcagttc gaggccgtgt tcgagcactc gcccatcggc aaagccctcc
     1321 ttgggacgga tgggcagttt ctcagggtca accgcgcgtt ctgccggatt gtcggcgcga
     1381 cgggcgagga gctctgcgcg cgccggctct acgacatcct tcggggagac gacgcggggt
     1441 tcattgcggt gggagccgag cgattgctcc ggggcgaggt cgaaagctat gcctgcgaga
     1501 cggggcttct ccggagcggc gccgaccttc tttgggcgct gctcgggctg tcgccggttc
     1561 ccgatgcgca cggacagccc gtccagatga tcgctcagct ccaggacatc acggaggttc
     1621 gcgcctatcg cgaaaggctg gaagatttgg tggcccggcg gacgcgcgag ctgcaccacg
     1681 cgaaggacga ggccgaacgg gccagccagg ccaagagctg gttcctgacc cacatgtctc
     1741 acgagattcg cagcccgttg caggtgatgg tcttgaacgc ggcgacgctc gagggtgatc
     1801 ggtctttgac tcccgagcag gtgagctgca tcgagagcat ccagagcagc ggcgagcatc
     1861 tcgcgacgct catcaacaac gtcctcgaca tgtcgaccgt cgagtccgga cgactggcgc
     1921 tcgtcgaaac ccgcttcgac ttgaccgcga tcctcgatga aacggctcag atgttcgccg
     1981 cgcaggcggc cgccaatggc acgagcctgc gcgtcgaggt cactcccggg ctacccgcgt
     2041 ccctgctggg agacggcggc aaggtcaaac agattctcat caacctggtg agcaacgcga
     2101 tcaagttcac ccaccggggc tccgtccgca tcgcggcgac ctgcagtccg gcggcacatc
     2161 cgacacccct ggtggagatc gtcgtcgccg acacgggcat gggaattggc aaccgaaacc
     2221 tggcgcgaat attccaaccc ttcgagcaac tcgaggccgg ggccaacacg ggcggcaccg
     2281 ggcttggtct caccatcagc ctcgcgtacg cccggttgat gggaggcgac atcaccgtcg
     2341 agagccggcc cggcgcaggg agtcggttca agctg
//
LOCUS       DASWVF010000158         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_182616, whole genome shotgun sequence.
ACCESSION   DASWVF010000158 DASWVF010000000
VERSION     DASWVF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182616"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VGD55_02705"
     CDS             complement(<1..1087)
                     /locus_tag="VGD55_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869284.1"
                     /translation="MPLNSQASSSTLSLANSHPTSAEPAGRSHKSAALAIILLAQLMV
                     VLDATIVNIALPKIQTALSFSRTDLSWVLNAYSLTFGGLLLLGARSGDLFGRRRVFLA
                     GIALFTAASLAGGFTTSSGLLLLARSVQGVGAAFASPSALALLMVMFREGAERTRAIA
                     LYTAVSIGGAAVGLVAGGVLVEWTSWRWVFFVNVPIGIVLLVLARRDLPETDRHTGRV
                     DLPGALTSTIGMAALVFGFVRAAAAGWQAGSTIGAFTVGIVLLAAFALIESRVATPIT
                     PLRLFADRDRVFSYLARLVLVGAMFGMFFFLTQFLQDVLGYSPLVSGLAFLPLTVALF
                     GASQLAARVLLARFGPKRLMVGGLTTST"
     gene            1190..1777
                     /locus_tag="VGD55_02710"
     CDS             1190..1777
                     /locus_tag="VGD55_02710"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0869285.1"
                     /translation="MSAVTTPDAADRALRADAKRNRDALLESAGQAFAEHGTAASLED
                     VVRRAGVGIGTLYRHFPTRDALVEAAYRHGVEQLCDSANHLLATQAPDVALEAWMLDF
                     VGYVATKRGLAGTLKLSAPDVHAELFAYVHERLRRAIDSLVNAAAAAGKIRSDVDGFD
                     LIRALGGIDRPCAPTCIEPTRSISAAGPSKPIGRP"
     gene            1831..2154
                     /locus_tag="VGD55_02715"
     CDS             1831..2154
                     /locus_tag="VGD55_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869286.1"
                     /translation="MEDLDSFIKSWAVFDSRHRPLGRAVALVDEKTAVLLLLRGPPDL
                     EVAHQTRYLLHTAVVADLIRHGVRHLVVESIIGAPEGLKYFAARLGYRACRIRVVQYQ
                     AVGAP"
BASE COUNT          441 a          699 c          716 g          330 t
ORIGIN      
        1 tcgtcgaggt ggtcagcccc ccgaccatca gccgcttcgg gccgaaccgg gccaacagca
       61 ccctggccgc tagttgcgac gccccgaaca acgcgaccgt caggggcagg aatgcaagac
      121 cgctgacaag cgggctgtag cccaagacgt cttgaaggaa ctgcgtcaag aagaagaaca
      181 tcccgaacat cgcaccgact aggaccagcc gcgcaaggta ggagaaaacc ctgtcacggt
      241 cggcaaaaag tcgcagcggc gtgatcggcg tcgcaaccct tgactcgatc agtgcgaagg
      301 cagcgagcag aacaatgccc acggtgaacg cgccgatcgt gctgccggcc tgccaacccg
      361 ccgcggcggc cctgacgaag ccgaacacga gcgcggccat accgatcgtc gaggtgagcg
      421 cgcccggcaa gtcgacccgg ccggtgtggc ggtcggtctc aggcaggtcg cgtcgagcca
      481 gcactagcaa cacgatgccg atgggaacgt tgacgaagaa cacccagcgc cacgacgtcc
      541 attcgacgag aacgccaccc gcgacgaggc ccacggccgc gccaccgatc gacacggctg
      601 tgtacagcgc gatcgcgcga gtgcgctctg cgccttcacg gaacatgacc atcaaaagcg
      661 cgagggcgga cggcgaggcg aacgcagcac cgacgccttg caccgagcgt gcaagcaaga
      721 gcaggccgga cgacgtggtg aagccgccgg cgagagaggc ggccgtgaac agggcgatgc
      781 cggcaaggaa cacccgacgt cggccgaaaa ggtcaccgct tcgggccccg aggagcaaca
      841 ggccgccgaa ggtaagggag taggcgttga gcacccacga gaggtcggtg cggctgaagg
      901 acagcgcggt ttggatcttg ggaagggcga tgttcacgat cgtcgcgtca agcaccacca
      961 tcagctgggc gagcaagatg atcgcgagag cggccgactt gtggctgcgc ccggctggct
     1021 cggcggaggt tgggtgggaa ttggcgagcg aaagcgttga tgaactggcc tgcgagttca
     1081 gtggcattcc aggcctcctt agtggatgat ggagtcaagc ggagggtgcc tcctgtaact
     1141 atacggaggc cgcctccggt tagcaagtac atttcaaagg aggcttggca tgagcgcggt
     1201 gacgactccc gacgccgctg accgggcact acgagccgat gccaagcgga atcgcgacgc
     1261 tctcctcgag agcgcgggtc aggcttttgc cgagcacggc accgcggcct cactcgagga
     1321 cgttgtgcgg cgggccggcg tcggaattgg caccttgtac aggcatttcc ccacgcgcga
     1381 cgcgctcgtc gaagctgcct atcgccacgg cgtcgagcag ttgtgtgact cggccaacca
     1441 cctgctggcc acgcaagccc ccgacgtcgc gctcgaagcc tggatgctcg acttcgtcgg
     1501 atacgtcgca acgaaacgcg gcctcgccgg cacgttgaaa ctgtccgcac ctgatgtgca
     1561 cgcggagctg ttcgcctacg tccacgaacg gctacgccgc gcgatcgact ccctcgtcaa
     1621 tgcggctgct gccgcaggaa aaatccgctc cgacgtcgac ggcttcgatc tcattcgcgc
     1681 actgggcggc atcgacaggc cctgcgcacc aacctgcata gagccgacaa gatccatctc
     1741 agctgccggc ccgtcgaaac cgattggcag gccctaaaga gtgcgctcag tgaagggccc
     1801 ttcgcgagcc ttgctatgaa gctcgacgcg atggaggacc tcgactcgtt catcaagtcc
     1861 tgggcagtgt tcgattcgcg gcaccgacca cttggccgag cagttgcgct cgttgacgag
     1921 aagaccgcag tgctgttgct cttgcgtggt ccgcccgacc ttgaggtggc gcatcagacg
     1981 cgttatctac tacacaccgc ggtggtggcc gacctcataa ggcacggtgt gcgccatctt
     2041 gtcgtcgagt cgattatcgg cgcgcccgag gggctgaagt actttgccgc gcgcctcggt
     2101 taccgcgcgt gccggatccg cgtcgtgcag tatcaggccg ttggcgcgcc gtagcgcagc
     2161 ccgtcgagca acaacgtcac gagacg
//
LOCUS       DASWVF010000159         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_29731, whole genome shotgun sequence.
ACCESSION   DASWVF010000159 DASWVF010000000
VERSION     DASWVF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29731"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VGD55_02720"
     CDS             complement(<1..1604)
                     /locus_tag="VGD55_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869287.1"
                     /translation="MLRATLRNVFAHKVRLLLTGLSVVLGVGFLAGTLVFTDTLKATF
                     NGLIGRTSTNLSVVVRATSDFSSTDIGASSARALVPNSMVPGVKAVSGVQQAVGEIQG
                     IDLLVTQAGKAVQPKSPGPPTLAVSWTPANFGSLAFVQGAGPTGPGQVAIDQSAANSY
                     HLNVGDHVTVQTQGAPIQATIVGIVTVGGSSNLAGAVLSVFDPTTAAQVAGKPGFVNQ
                     IDVQAAKGVSQEELAARIAPTLPKGFEAVTAKAVNKEQSDSISKALGFFNVFLLIFAG
                     VALFVGLFIILNTFTMLVAQRTRELALLRAIGASRRQVMSAVLGEAFVVGVVASTVGL
                     AVGLLVAVGVRGLLDAVGVSMPAGSLVIKTHTIVASYIVGVLVTLVAAWFPSFRASRI
                     PPIAAMRDGVALPERSLRIRAIIGGVLTLIGAVSIAGSIRGSSGGAAAKVGIGILLVM
                     IGMWVMSALISRPVVRAIGAPLIWWYGLPGRLGWLPGRLGWRNDAVSARLGHSNAVRN
                     PRRTAATSASVMIGLALVTMLSVLAAS"
     gene            complement(1604..2425)
                     /locus_tag="VGD55_02725"
     CDS             complement(1604..2425)
                     /locus_tag="VGD55_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869288.1"
                     /translation="MSPVQPGGAPGGTAAAARALRLTKIYGEGETTVAALRDVTVDFA
                     RGHFSAIMGPSGSGKSTLMHCLAGLDSITSGSAIIGDVELNTLNDKALTLLRRKEVGF
                     IFQSFNLIPTLTAMENITLPMDIAGRKPDRAWLDAVIDAVGLRDRLHHKPSELSGGQQ
                     QRVACARALASRPTIIFADEPTGNLDSRASAEVLDLLQQSVREMGQTIVMVTHDPWAA
                     SYADRVVFLADGQVIDEMRDPTQSAVLDRMSRLEARSRDAQDQDAPTVEARSQDA"
     gene            complement(2628..3539)
                     /locus_tag="VGD55_02730"
     CDS             complement(2628..3539)
                     /locus_tag="VGD55_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0869289.1"
                     /translation="MTAVDMIAGELAAVEPRQWALSPSRAGDFMTCPLLYRLRVIDRL
                     PEPSSPVTTRGTLVHGVLERLFDSPAAERTIERASGLLEPEWQRLLEERPELVDLFAD
                     LPDGGASTREQVAAWLASARVLLETYFRLEDPTRVEPAEREVLVEAALEGGVRLRGFV
                     DRVDVAPGGQTRIVDYKTGNSPSELFEAKALFQLKFYALVMWRVSGSIPAVLQLMYLG
                     DGQILRYAPDEADLLATERKVRALWAAIERGLANRDFPERPSRLCDWCAFKALCPAFG
                     GVTPAYPEPTDAAVPVDRPAEADDITP"
     gene            3635..>4291
                     /locus_tag="VGD55_02735"
     CDS             3635..>4291
                     /locus_tag="VGD55_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0869290.1"
                     /translation="MAAPEQQQPGQRTSGPVQPSGPEPKPRRRGSGLQGQLIGRPFGV
                     PIYISPSWLLFVGVITVWFAPVVATEVPGIGSPRYLVAATFGVFLGLSVLAHEIAHCV
                     VARRFRIPIERITLSFLAGHSVLAEEPKTPGRASAVAAAGPIANLVIAVGSWVALRQF
                     PVGGIGAILAAGLAWSNAVVGVYNALPGLPLDGGQLLRAAIWRVTGSVRRGTIGAAWA
                     G"
BASE COUNT          749 a         1425 c         1435 g          682 t
ORIGIN      
        1 gacgccgcga gcaccgagag catcgtcacg agtgcaaggc cgatcatgac ggacgccgag
       61 gtggccgcgg tccgtcgcgg attgcggacg gcattcgagt gcccgaggcg ggcggagaca
      121 gcgtcgttgc gccagccaag ccgcccaggc aaccagccaa gccgcccagg caagccgtac
      181 caccagatca atggcgctcc gatcgcgcgc acgaccggcc ggctgatcaa cgcgctcatc
      241 acccacatgc cgatcatcac cagcagaatg ccgatgccaa ccttggcggc cgcgccgccg
      301 ctactacccc gaatcgaccc agcgatcgag accgcgccga tgagcgtgag gactccgccg
      361 atgattgccc ggatccgcag tgagcgctct ggaagggcga cgccgtcacg catagcggcg
      421 atcggcggaa tgcgcgatgc ccgaaacgac gggaaccacg cggcgacgag agtgacgaga
      481 acgccgacga tgtaggaggc gacgatcgtg tgcgtcttga tgacgagcga gcctgcgggc
      541 atcgagacgc cgacggcgtc gagcaggccg cgaacgccga ccgcgacgag gagaccgacc
      601 gcgagaccga ccgtcgatgc aaccacgccg accacgaacg cctcaccgag cacggcgctc
      661 atcacctgcc gccggctcgc accgatcgcg cgcaagagtg cgagttcacg ggtgcgctgg
      721 gcgaccagca tcgtgaacgt gttcaggatg atgaaaagcc caacgaacaa cgccacaccg
      781 gcgaagatca acaggaacac attgaagaaa ccgagcgcct tgctgatcga atcggattgt
      841 tccttattca cagccttcgc cgtgaccgct tcgaacccct tcggcagcgt cggcgcaatc
      901 cgggccgcga gctcctcttg cgagacgcct ttcgcggctt ggacgtcgat ctggttcacg
      961 aatcctggct tgccggcgac ctgcgcagca gttgtcggat cgaacacact cagcaccgcg
     1021 ccggccaagt tgctgctgcc gccgacggtg acgatgccga cgatggtggc ttgaatcggc
     1081 gcgccctgcg tctgcacggt gacgtgatcg ccgacgttca ggtgatacga attcgcggcg
     1141 ctctggtcaa tcgcgacctg acccgggccg gtcgggcctg ctccttgaac gaacgcgagg
     1201 ctgccgaagt tggccggtgt ccacgagacc gcgagtgtgg gcggcccagg agatttcggt
     1261 tgcaccgctt tgcctgcttg cgtgacaaga aggtcgatgc cctggatctc accgacggcc
     1321 tgctgcacgc ccgacactgc cttcacacca ggcaccatcg agttcggcac aagagcgcgc
     1381 gcgctggagg cgccgatgtc ggtgctcgaa aaatcagacg ttgctcggac gacgacactg
     1441 aggttcgtgc tcgtgcgacc gatcaggccg ttgaacgtcg ctttcaaggt gtcggtgaag
     1501 acgagggtgc ccgccaagaa gccaacaccc aggacaaccg agagtccggt cagtagcagg
     1561 cgcaccttgt gagcgaacac gttgcgcagc gtggctcgca gcatcaggcg tcctgactcc
     1621 gagcttccac agtcggggcg tcctggtcct gagcatcgcg actgcgagct tcaagccggc
     1681 tcatgcggtc aaggaccgca gattgggtgg gatcgcgcat ctcgtcgatg acctgcccgt
     1741 cggcgaggaa cacgacacgg tcggcatacg acgccgccca ggggtcgtgc gtgaccatca
     1801 cgatggtctg acccatctcg cgcaccgact gctggaggag atccagcact tcggcgctgg
     1861 cgcgcgagtc gaggtttccc gtgggctcgt cggcgaagat gatcgtgggt cgcgatgcga
     1921 gcgctcgtgc gcacgcgacg cgctgctgct gaccgccgga gagctcagag ggcttgtggt
     1981 gcaagcgatc gcgtaacccg accgcatcga taacggcgtc gagccaggcc cggtcgggct
     2041 tgcggccggc aatgtccatc ggcagcgtga tgttctccat agccgtcaac gtggggatca
     2101 agttgaacga ctggaagatg aagccaacct cctttcggcg cagcagagtg agtgccttgt
     2161 cgttgagcgt gttgagttcg acgtcgccga tgatcgcgct gcccgacgtg atgctgtcga
     2221 ggcctgcaag gcaatgcatg agggtcgact tgcccgagcc cgaggggccc atgatcgcgc
     2281 tgaaatgacc gcgcgcgaag tcgacggtga catcgcgcag tgcggcgacc gtcgtttcgc
     2341 cctcgccgta aatcttcgtc agacgcagcg cccgtgcggc cgctgcagtt ccgcccgggg
     2401 ctccgcccgg ttgaacggga ctcatggtcg tcacgaacga ctcctcttgc cggtggaatg
     2461 ggcggggcag gtgcgacaaa tgcgaacctg cggcgtgacg gcgtgcctag cgaccgatca
     2521 cgaggtcgct tgcccacgct acctggacgt cgggatggtg cggatcggac ggacgccacc
     2581 gatcggcagt gacaaccgtc actggcttat ggcgtgatgt cggcggctta tggcgtgatg
     2641 tcgtcggctt ctgccggccg gtcgacgggc actgccgcgt cagttggctc gggataggcg
     2701 ggcgtcacgc caccgaacgc ggggcacagc gccttgaatg cgcaccagtc acacaaccga
     2761 ctcggacgct ccggaaagtc gcgattcgca agtccgcgct cgatcgccgc ccacagcgcc
     2821 ctgactttgc gctcggtcgc gagcaggtcg gcttcgtcgg gcgcgtaccg caggatttgc
     2881 ccgtcgccga ggtacatcag ttgcagcaca gccggaatcg agccgctgac acgccacatc
     2941 accagtgcgt agaacttcag ctggaacagc gccttcgcct caaagagctc cgacggcgag
     3001 ttgcccgtct tgtagtcgac gatgcgggtc tggccgccag gggcaacgtc gacccggtcg
     3061 acgaacccgc gcaaccgcac gccgccctca agggcagcct cgacgagcac ttcgcgctcc
     3121 gccggctcga cgcgggtcgg atcttcaaga cggaagtagg tctcgagcag cacgcgcgcg
     3181 ctcgcgagcc acgccgccac ctgctctcgg gtcgaggcgc ccccgtcggg cagatcggcg
     3241 aataggtcga cgagttctgg tcgctcttcg agcaaccgtt gccactcagg ttcgaggagc
     3301 ccagacgcgc gctcgatcgt gcgctcggca gccgggctgt cgaacaatct ctcgaggacg
     3361 ccgtggacca gcgtgccccg cgtcgtgacc ggacttgacg gctcaggcaa ccggtcgatg
     3421 acgcgcagcc ggtagagcag cgggcaggtc atgaagtcgc cggcgcggga cggcgataac
     3481 gcccactgcc gaggttcgac agccgctagc tcacccgcga tcatgtcaac tgcagtcata
     3541 cgggcagcgt aggcccgccc cacgacactt cgggggtgcc tcgcctgcgc tgtggacgga
     3601 cggcaggggc tgggtgtggc aataccatcg agacgtggcc gccccggagc aacagcagcc
     3661 cgggcagcgc acaagcggtc cggtacaacc gagcggcccg gagccgaagc cccgtcgccg
     3721 cggctcgggc cttcagggcc aactcatcgg ccggccattc ggcgtcccga tctatatctc
     3781 accgagctgg ctcctcttcg tcggcgtcat caccgtctgg ttcgccccgg tagtggcgac
     3841 ggaggtgccc gggatcggct ccccccgcta cctcgtggca gcgacgttcg gggtgttcct
     3901 ggggctctcg gtgcttgccc atgagatcgc ccactgtgtc gtggcccgcc ggttccggat
     3961 cccgatcgag cgcatcacgc tcagcttcct ggctggccac tcggtgctgg ccgaggagcc
     4021 caagaccccg ggccgcgcct cggccgtcgc tgcggccggc ccaatcgcca acctcgtcat
     4081 cgccgtcggc tcatgggtcg cgttgcggca atttcccgtc ggaggcatcg gggcgattct
     4141 ggccgccggg ctggcgtggt cgaacgcggt cgtgggtgtc tataacgcgt tgccgggtct
     4201 gccgctcgac ggaggccagt tgctgcgcgc ggctatctgg cgcgtcacgg gcagcgtccg
     4261 gaggggaacg atcggcgccg cctgggccgg c
//
LOCUS       DASWVF010000160         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_386561, whole genome shotgun sequence.
ACCESSION   DASWVF010000160 DASWVF010000000
VERSION     DASWVF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386561"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1915)
                     /locus_tag="VGD55_02740"
     CDS             complement(<1..>1915)
                     /locus_tag="VGD55_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0869291.1"
                     /translation="DGGSTGTGAAGTAAAGGGEGTAGTGTSAAGAGSGGAGYSASGSG
                     TTTSNSSLLVGQSASANVPVDQALTPQTITSTSASSSGTAASSAAVSGGSSSFGSGSG
                     TSTSPFVGALQTWAVPSVDKRVGDVATVLNSTVVPRVISSQNGHPGVTLLIQKQADAS
                     EVTVSDEILGALPQLQRQFPGVQFQVAHVQSIFTEEQVQSVEHTLVEGIILTAIVMLF
                     FLGSWRNAIVVLIAIPTSLGVTLFVMQMLGLTLDTISLMAMTLVIGILIDDSTVVLEN
                     IERHHAAGEEPADAALNGRSEIGLAAIVITLVDVVVFLPIAFAGGQVGQNLHEFAIVI
                     TVATVTSLLVSFTLTPMMAGLWSLKSTWRPWRPIRWFDDRFDGFREHYAERWLPAALG
                     RPWPIVIAAVVLVVLAYVLVPTGLVGEEYIPAEDQGIIYAQVAFPAGHPLLQTQAAMS
                     KLEAVTKQVLPTDDLQYEVTIAGAYSAAFGGFVQEGNVGQISIYLNPKRSRSTYDDVT
                     ALEQRYRKVEPGASITVAAATQQGGGNQQQIDELVSTGGSGGDPTRYAAKVYAALRAT
                     PGAAGVQNSATNAAPQMEIDYNRPALQALDVSTGTASAAVEAAFGGDIATQIETPVNG
                     LTDIEVMYPRSAQG"
BASE COUNT          316 a          634 c          639 g          326 t
ORIGIN      
        1 agccctgggc cgaacgcgga tacatgacct cgatgtcggt gagaccgttc acgggcgtct
       61 cgatctgcgt cgcgatgtcg ccgccgaacg cggcctccac ggccgccgaa gccgtcccgg
      121 tggacacgtc gagcgcctgc agggcggggc ggttgtagtc gatctccatc tgcggcgccg
      181 cgttggttgc actgttctgg acaccggcag cgcccggtgt ggcgcgcaac gccgcgtaga
      241 ccttcgcggc gtagcgcgtc gggtcgcccc cgctgccgcc agtcgacacc agttcgtcga
      301 tctgctgctg gttgccgccg ccctgctggg tcgcggccgc gacggtgatg gaagcgccgg
      361 gttcgacctt tcggtagcgc tgctcgagcg cggtcacatc gtcgtacgtt gagcggctgc
      421 gcttggggtt gaggtaaatc gaaatctggc cgacgttgcc ctcttgcacg aacccgccaa
      481 aggccgcgct gtacgcgccg gcgatcgtga cctcgtactg cagatcgtcc gtcggcagta
      541 cttgcttcgt cacggcttcg agcttgctca tcgccgcttg ggtctgcagc agcggatggc
      601 cggcgggaaa ggcgacttgc gcgtagatga ttccctgatc ttcggccggg atgtattcct
      661 cgccgacgag cccggtcgga accagaacgt acgccagcac aacgagcacg acggcggcga
      721 tgacgatcgg ccacggacgc cccagcgcgg ccggcagcca acgctcggcg tagtgttcgc
      781 gaaagccatc gaagcggtcg tcgaaccacc gaatgggccg ccagggtcgc cacgtcgact
      841 tgagcgacca gagccccgcc atcatcggcg tcagggtgaa cgaaacgagc agcgaggtca
      901 cggtcgcgac ggtgatgacg atcgcgaact cgtgcaggtt ttgccccacc tgtccaccgg
      961 cgaacgcgat ggggagaaag acgaccacgt cgaccagcgt gatgacaatc gcggccaagc
     1021 cgatttcgct gcgtccgttg agcgcggcgt cggcgggctc ttccccggcg gcgtgatgcc
     1081 gctcgatgtt ctcgagcacg accgtcgaat cgtcgatcag gattccgatc accagcgtca
     1141 tcgccatcag cgagatggta tcgagcgtga gacccagcat ctgcatgacg aacagggtga
     1201 cgccgagcga ggtcgggatg gcaatcagga cgacgatcgc attgcgccac gaaccgagga
     1261 agaagagcat cacgatcgcg gtgaggatga tgccctcgac cagcgtgtgc tcgacgctct
     1321 gcacctgctc ttcggtgaag atcgactgta cgtgggcgac ttggaactgc acgccgggaa
     1381 actgccgctg tagttgcggc aacgcgccga ggatctcgtc ggaaacggtc acttcgctgg
     1441 cgtcggcctg cttctgaatc agcagcgtca ccccggggtg cccgttttgc gacgatatga
     1501 cgcgcggcac gacggtcgag ttcaggaccg tcgcgacatc gccaacgcgc ttgtcgacgc
     1561 tgggaactgc ccaggtctga agcgctccga cgaagggcga ggtcgacgtt cccgatccgc
     1621 tgccgaacga actcgacccg ccggaaaccg ccgcgctcga cgcggccgta ccggagctgc
     1681 tcgcgctggt cgatgtgatc gtctgcggcg tcagtgcctg gtcaaccggc acgttggctg
     1741 aggcactctg tccgaccagc aacgaactgt tgctcgtcgt ggtgccgctg ccggacgcgg
     1801 agtaacctgc gccgcccgag cctgctcccg cggcactggt tcccgtaccg gccgttccct
     1861 cgccgccgcc ggccgccgcc gttccggctg cgccggtgcc ggtcgaaccg ccgtc
//
LOCUS       DASWVF010000161         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_335406, whole genome shotgun sequence.
ACCESSION   DASWVF010000161 DASWVF010000000
VERSION     DASWVF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335406"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="helR"
                     /locus_tag="VGD55_02745"
     CDS             complement(<1..1382)
                     /gene="helR"
                     /locus_tag="VGD55_02745"
                     /inference="COORDINATES: protein motif:HMM:NF041254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor ATPase HelR"
                     /protein_id="HEY0869292.1"
                     /translation="MNPLTASAFDLPDHLSHKDDPTLIAGDEQHFAALAESLGQSIAD
                     LSGRLDDLRKAPGGIGQEAMDRDMEIHRVTARLRTLRRFGLDLCLGHIVSADDREPVY
                     IGRLGLTDRAGRRLLLDWRSPAAEPFFGATHANPMGLASRRRYRWTLGRISDYWDEVF
                     TDDGFEGHAAALDDQSAFIASLGSSRSPRMRDVLATIAADQDAIVRAGSHGSLVVDGG
                     PGTGKTVVALHRAAYLLYSDPRLGHHRGGVLFIGPHQPYLAYVADVLPSLGEEGVQTS
                     TLRDLIAEGAAARTETDADVAALKSSANLVHAIETAVRFYEEPPTKGITVSTDELDLW
                     LSAEDWAEAFASPDPGTAHNEARDLIWDELVTILMDKDDGESPDLLRRSLLRNDELRA
                     TFNRAWPLIEATDLVADLWSVPAYLRKCAPWLTPAEIRSLQREDAQAWTISDLPLLDA
                     ARQRLGDPETS"
     gene            complement(1505..>2050)
                     /locus_tag="VGD55_02750"
     CDS             complement(1505..>2050)
                     /locus_tag="VGD55_02750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0869293.1"
                     /translation="SQADATRDGAELFAALDHDAVWQHIAGRPTDAAGYAKLLDDGYA
                     AGRLPWIVRLAVPYVDLPAGAVVGTSSYLDVSASDARLEIGFTAYTPAAWAGVVNPET
                     KLLLLGYAFDVLGAGRVQLKTDVRNARSQQAIARLGAQYEGVLRRYQRRADDTVRDTV
                     IFSILAQEWPDVRAGLNARLA"
BASE COUNT          338 a          659 c          700 g          353 t
ORIGIN      
        1 gacgtctccg ggtcgccgag tcgctgccgt gctgcgtcga gcagcggcag gtcggagatt
       61 gtccaggcct gcgcgtcttc acgttggagc gagcgaatct ctgcgggcgt gagccacggc
      121 gcgcacttgc gcaggtaggc cggcaccgac catagatcgg cgacgaggtc tgttgcttcg
      181 atcagtggcc atgctctgtt gaaggtcgcg cgtaattcgt cgttgcgtag caacgacctg
      241 cggagcaggt cgggcgattc gccgtcgtcc ttgtccatca ggatcgtgac gagctcgtcc
      301 cagatcaggt cgcgcgcctc gttgtgcgca gtgccggggt ctggcgatgc aaatgcctct
      361 gcccaatcct cggcgctaag ccacagatcc aactcgtcgg ttgaaaccgt gatgcccttg
      421 gtgggcggct cctcgtagaa cctgaccgcc gtctcgattg cgtgcaccag gtttgcggac
      481 gacttcagcg cagcgacgtc ggcgtcggtc tcagtccgcg ccgcggctcc ttcggcgatg
      541 aggtcgcgca gcgtggaggt ctgcacgcct tcctctccaa gacttggcag gacgtcggcc
      601 acgtaggcca ggtagggctg gtgcggaccg atgaacagca cgccgccccg gtggtgaccc
      661 aggcgcgggt cggagtagag caggtaggcg gcccggtgca gcgcgaccac ggtcttcccg
      721 gtacccggac caccgtcgac gacgagactg ccgtgagaac ccgcgcggac gatggcgtct
      781 tgatcggccg cgatggtggc gagcacatcg cgcatccggg gcgaccggct gctgccgagg
      841 ctggcgatga aggccgactg gtcgtcgagc gcggcggcat ggccttcgaa cccgtcgtcg
      901 gtgaagacct cgtcccagta gtcgctgatc cggccgaggg tccagcgata cctgcgacgg
      961 ctggccaggc ccatcgggtt ggcgtgcgtc gctccgaaga acggctcggc cgctggggag
     1021 cgccaatcga gcagcagccg acggcctgct ctgtcggtga gcccgagccg cccgatgtac
     1081 accggctcgc ggtcgtcggc gctcacgatg tgtcccagac acaagtccaa accgaaacga
     1141 cgcagcgtgc gcaggcgcgc ggtcacccgg tggatttcca tgtcccggtc catcgcttcc
     1201 tggccgattc cgccaggggc tttgcgcagg tcgtcgagac ggcctgagag atcagcgatc
     1261 gattgtccga ggctttccgc aagcgccgcg aagtgctgct cgtcgccggc gatgagcgtc
     1321 gggtcgtcct tgtggctgag atggtcggga aggtcaaaag cgctggcggt caaggggttc
     1381 acgtcatctc ctatccgagg ttcgtggcct cgggtggcga tagtgcggca agaccccggt
     1441 cttgcggcaa gccccctgct gcgctatacg ttaagaatgg aaggagtgag tactcctccg
     1501 ttccctaagc gagacgggcg tttaagcccg cacgcacgtc gggccactcc tgcgccaaga
     1561 tcgagaagat gaccgtgtcg cgcacggtgt cgtcggccct gcgctggtag cggcgcagca
     1621 cgccctcata ctgcgcaccg agccgtgcga tcgcctgctg cgaccgagca ttgcgcacat
     1681 cggtcttgag ctgaacacga cccgcaccga ggacgtcgaa ggcgtaaccc aagagcagca
     1741 acttggtttc cgggttcact actccggccc aggccgccgg cgtgtaggcg gtgaagccaa
     1801 tctcaagccg agcatcggac gccgagacgt cgaggtacga cgacgtcccg acgaccgccc
     1861 ccgctggaag atctacgtac ggaaccgcga ggcgcacgat ccacggcaac cgaccagcgg
     1921 cgtaaccatc gtcgagcagc ttggcgtagc cggccgcatc ggttggtcga cccgcaatgt
     1981 gctgccatac ggcgtcgtgg tcgagggcgg cgaagagttc tgcgccgtcg cgcgtcgcat
     2041 cagcctggct
//
LOCUS       DASWVF010000162         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_208323, whole genome shotgun sequence.
ACCESSION   DASWVF010000162 DASWVF010000000
VERSION     DASWVF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208323"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGD55_02755"
     CDS             complement(<1..280)
                     /locus_tag="VGD55_02755"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0869294.1"
                     /translation="MRVNPWADAGVACVAQWDRVADAVDALPDESFELPTALPGWRVA
                     DLVAHLSMCAGLAARWAAEPAPERIEAGVGPYLLSLAAAASAVDEGTRA"
     gene            complement(280..2553)
                     /gene="purL"
                     /locus_tag="VGD55_02760"
     CDS             complement(280..2553)
                     /gene="purL"
                     /locus_tag="VGD55_02760"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0869295.1"
                     /translation="MSETVDHPATIDSVTRAAATPDAVLPFAALGLKDDEYARIVAIL
                     DRRPTSCELAMYSVMWSEHCSYKSSKRHLRQFGERIPPQAKDILLVGIGENAGVVDVG
                     QGFAVTFKAESHNHPSYVEPYQGAATGVGGIVRDILTMGARPVAVMDSLRFGPADAPD
                     TARVLPGVVAGIGGYGNCLGLPNIGGEIVFGPSYYGNPLVNALAVGVMRTDGIKLATA
                     PGPGNKVVLFGARTGGDGIGGASVLASAAFDAEGQRKRPSVQVGDPFAEKVLIECCLE
                     IFAADLVVGIQDLGAAGLSCATSELASAGTGGMRVVLDTVPLRDPTLAPEEILMSESQ
                     ERMCAVVEPAKIPAFMEICAKWDVNATVIGEVTDGDRLEIYWRDELVVDVPPRTVAHD
                     GPVYDRPISCPADLDALNADDPGALKRPASGAELRDTLLALVAAPNLAGKGWVTDQYD
                     RYVMGNTALAMPDDAGVIRVDDSTDLGLALALDGNGRYTRLDPYTGAQLALCEAYRNV
                     AAAGARPLAITDCLNFGSPEDPEVMWQFTEAIRGIADGCLALGIPVTGGNVSFYNQTG
                     STPINPTAVIGVLGVIDDVRLRRSIAFAADDELIFLLGTTRDEFGGSEWAHIVHGHLG
                     GRPPVVDFDAERALAGLLVEAASDAVVSAAHDLSDAGLAQALVESCLRGGRGARISLP
                     GDPFVALFAESAARAIVAVPPAKVPALLAMAAARGVPCQEIGVTGGDTLDIADTFAVE
                     VATLRAASERTFPALFG"
     gene            complement(2550..3239)
                     /gene="purQ"
                     /locus_tag="VGD55_02765"
     CDS             complement(2550..3239)
                     /gene="purQ"
                     /locus_tag="VGD55_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0869296.1"
                     /translation="MAPLVGVVTFPGSLDDRDALRAIKAAGAEPISLWHKDPSLHGAD
                     AVVLPGGFSYGDYLRCGAIARFSPVMDLVIAGAREGLPVLGICNGFQVLCEAHLLPGA
                     LTRNADLHFRCADQPLVIENTTTAWTNGYEQGARVVIPVKNGEGRFVADEHTLDQLEA
                     DGLVVARYAGTNASANRNGSVGDIAGITNERGNVVGLMPHPEHAIDPLTGPSADGLTF
                     FTSIVKALIAA"
     gene            complement(3239..3484)
                     /gene="purS"
                     /locus_tag="VGD55_02770"
     CDS             complement(3239..3484)
                     /gene="purS"
                     /locus_tag="VGD55_02770"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0869297.1"
                     /translation="MARVVVDVMLKPEILDPQGQAILGALGRLGRSGVASVRQGKHFE
                     IELDGEADPAALAQVDELAASFLSNPVIEDFVVRLDV"
     gene            3599..6205
                     /locus_tag="VGD55_02775"
     CDS             3599..6205
                     /locus_tag="VGD55_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869298.1"
                     /translation="MTETGAERARLRQADGGAVDWRRWGTYLSERAWGSVREDYSADG
                     DAWASFPFEHARSRTYRWNEDGLAGFCDVDQTWCLGLAMWNCKDPILKERLFGLTNSQ
                     GNHGEDVKERYWFVDGTPTHTWMSWRYHYPQLAFPYDQLRTENARRSRLDPEYEIDDT
                     GAFAGGRYFDVGVDYAKAAADDLCMRITVHNPGAETATIELLPTLWFRNTWSWGLPKA
                     LPAPRITCDGPSLVAVHAKLGTLTLTGAPTDFVESVACDNETNTRLLYGVPGPPYPKD
                     GIGDHVVSGAPTVNPEGFGTKAALRYRLVLAPGETREVRVRLQAGPSTADLGAGFDAV
                     LTARKLEADGFYAGLPAMAMASDEPPDVTAGEAAIVRQAIAGLLWSKQFYHYDVQRWL
                     DGDPAGPPPPLGRGDIRNGKWTQLNNHDVLLMPDTWEYPWYASWDLAFHCVVLSHADP
                     AFAKQQLLLLLREWYMHPNGQLPAYEWNFSDVNPPVHAWAALRVFEVSGGTDYDFLER
                     AFHKLLLNFTWWVNQEDHDGNNLFQGGFLGLDNIGPFDRSKLSPDAGELEQSDGTAWM
                     AMYCLDLLDMALVLATHDPTYEDVATKFFEHFAHIATAAQQQGLWDDGDGFFYDQLRM
                     PDGERRPIKVRSLVGLIAVCAVSHVDNALLAQLPGFASRLQWFRTNRPALTHCVVATE
                     DDDTMFAMLDPDQLRRLLSRMFDEAEFFSPHGIRSISAYHRDHPFEFSLDGQDFRVDY
                     EPGESSTGLFGGNSNWRGPVWLPLNALLVEALQRFDQHLGKDFLIEVPKGSGREITLG
                     QAADELAGRLISLLLPDSDGHLAASGNRVWPEGLLWFHEYFHGDTGAGLGASHQTGWT
                     ALLAHLVLTRRH"
     gene            6658..>8746
                     /locus_tag="VGD55_02780"
     CDS             6658..>8746
                     /locus_tag="VGD55_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0869299.1"
                     /translation="MSVIELPGSAREFERTPPQDINAEQSVLGGMLLSKDAIADVVES
                     LHGTDFYRPAHQTIFDVVLDLYGRGEPADAVTVAAELTKRGEIARIGGAPYLHTLISS
                     VPTAANAGYYAKIVAERAILRRLVEAGTRIVQLGYSGVGNEVDDIVDRAQAAIYDVTE
                     RRQSEDYSILESLLQPTMDEIEASGHRGGGLTGVPTGFSDLDSLTNGLHPGQLIVIAG
                     RPAMGKALALETPLPTPFGWTAMGEVVVGDWLMGADGRPTQVIAATGVLLERPCYEVE
                     FDDGSVIVADAQHQWLTETRASRRSPRTMTGRSSRSADPFAFGEVRTTQEIAETLRCP
                     TADARTNHSVPNTLPLQLDEQQLPVPPYTLGVWLGVGHFASARFTSDDPEIGWHIEAE
                     GLVVIPRGGRLYRLRLPSESDVDTRECAVCGKTFTPKTIAVRTCSQSCGGKSRSLGLP
                     PAATPTCMSCGAPTVSGFGRCTPCRLDHGSVGALLQRIGVLNNKHIPAEYLRASEEQR
                     RALLAGLLDTDGTVSPTGAVQITLTDARLARDVHELVVSLGYRCGFTSKAVNGHAAAS
                     ATAYTTSFTAADSPFRLERKRLALKERATKHTPRIGARFIVDVRPVPSVPVRCVEVGN
                     AAHLYLAGYSMIPTHNSTLGLDIARAASIRHNQTSVLFSLEMSKHEITMRLLSAEARI
                     PLQTMRTGQMREDD"
BASE COUNT         1541 a         2934 c         2861 g         1410 t
ORIGIN      
        1 gggcacgggt gccctcgtca accgcggatg cggctgccgc cagactcagc agatacggcc
       61 cgacgcccgc ctctatgcgt tcgggcgcag gctccgcggc ccaccgcgca gccaagcccg
      121 cacacatcga gaggtgggcg accaaatcgg ccacccgcca ccccggcaac gccgtaggca
      181 attcgaaact ctcgtccggt aacgcatcga cagcgtcagc gaccctgtcc cactgcgcaa
      241 cgcacgccac cccggcgtcc gcccacgggt tgactcgcat cagccaaaca aggcagggaa
      301 ggtacgttcg ctcgcggccc ggagtgtggc gacctcgacg gcgaaggtgt cagcgatgtc
      361 gagggtgtcg ccgccagtca cgcctatttc ctgacacggg acgccgcggg cagccgccat
      421 tgcgagcagc gcaggcacct tggccggagg aactgccacg atcgcccgcg ccgccgactc
      481 ggcaaacagc gcgacgaacg gatctcctgg caacgagatg cgcgcacctc gacccccgcg
      541 caggcacgac tcgacgagag cctgcgccaa cccggcgtcg gataaatcgt gggcagccga
      601 cacaacggcg tcgctggctg cctcgaccag caaacctgcc agcgcacgct ccgcgtcgaa
      661 atcgaccacc ggcgggcggc cacccaggtg gccgtgaacg atatgcgccc attcggatcc
      721 gccgaactcg tcgcgggtcg tgccgaggag aaagatcagc tcgtcgtcgg ccgcgaaggc
      781 gattgatcgg cgcaagcgca catcgtcgat gacgccgagc acgccgatca ccgccgtcgg
      841 gttgatcggg gtcgacccgg tctggttgta gaagctgacg ttgccgcccg tcaccgggat
      901 gccgagagca agacacccgt cggcgatccc gcgaatcgcc tcggtgaact gccacatcac
      961 ctcgggatcc tctggcgagc cgaagttcag gcagtcggtg atcgcgagtg gccgtgcgcc
     1021 ggcggccgcg acattgcggt aggcctcgca caacgcgagc tgggcgccgg tgtacggatc
     1081 caacctggtg tagcggccgt tgccgtcgag agcgagcgca aggccgaggt cggtcgaatc
     1141 gtcgactctg atcacgccgg cgtcgtcggg catggccagg gccgtgttac ccatgacgta
     1201 cctgtcgtac tggtcggtca cccagccctt gcctgcgagg ttaggtgcgg cgaccagcgc
     1261 gagcagcgtg tcgcgaagct cagcgccaga cgccggtcgc ttcaacgcac cggggtcgtc
     1321 ggcgttgagc gcgtcgagat cagcggggca ggaaatcggc cggtcgtaga cgggcccgtc
     1381 gtgcgcgacg gttcgtggcg ggacgtcgac cacgagctca tcgcgccaat agatctcgag
     1441 acggtcgcca tcggtcacct ccccaatgac ggtggcgttg acgtcccact tcgcgcagat
     1501 ctccatgaac gcagggatct tcgccggctc gaccaccgca cacatgcgct cttgtgactc
     1561 gctcatcagg atctcttcgg gagcaagcgt cgggtcgcgc agcggcacgg tgtcgagcac
     1621 gacccgcata ccgccggtgc ctgccgaggc cagctcggat gtcgcgcatg acaatcccgc
     1681 agcgcccagg tcttggatgc caaccacgag atcggcggcg aagatctcaa ggcagcactc
     1741 gatgagcacc ttctcggcga acgggtcgcc cacctgcacg ctcggtcgtt tccgctggcc
     1801 ctcggcgtcg aaggccgcgc tggccaacac cgacgcgccg ccgatgccgt cacccccggt
     1861 gcgggcgcca aaaagcacga ccttgttacc cggcccaggt gcggtcgcga gcttgatgcc
     1921 atcggtgcgc atcacgccga ccgcgagcgc gttgaccagc gggttgccgt agtacgacgg
     1981 accgaacacg atctcgccgc cgatgttggg cagcccaaga cagttgccgt agccgccgat
     2041 gcctgcgacg acacccggca agacgcgggc cgtgtcgggt gcgtcggccg ggccgaagcg
     2101 cagggaatcc atcactgcga ccggccgtgc gcccatcgtc aagatgtcgc gcacgatgcc
     2161 cccgacgccg gtcgccgcgc cttgatacgg ctcgacgtag ctcgggtggt tgtgcgactc
     2221 ggccttgaac gtcaccgcga agccctggcc gacgtcgaca acgccagcgt tctcgccgat
     2281 gccgacgagc agaatgtcct ttgcttgcgg cggaattcgt tcaccgaact gccgcagatg
     2341 ccgcttgctc gacttgtacg aacagtgctc gctccacatc accgaataca tcgcaagctc
     2401 acacgacgtc ggtcgccggt cgaggattgc gacgatccgc gcgtactcgt cgtccttcaa
     2461 accaagagca gcaaatggca ggacggcgtc gggcgtcgca gcagcgcgag tgaccgaatc
     2521 aatcgtggcc gggtggtcga cggtctcact cacgcggcga tcaatgcctt gacgatgctg
     2581 gtgaagaacg tcagaccgtc ggcgctcgga ccggtgagcg gatcgatcgc atgctcggga
     2641 tgcggcatca ggccgacgac attgccgcgc tcgttggtga taccggcgat gtcgccgacc
     2701 gagccgttgc ggttagcgct cgcgttggtg ccggcgtaac gagccacgac gaggccgtcg
     2761 gcttcgagct ggtcgagggt gtgctcgtcg gcgacgaagc ggccctcgcc gttcttcacc
     2821 ggaatcacca cccgcgcgcc ctgctcgtag ccgttggtcc aggcggtggt tgtgttctcg
     2881 atgaccagcg gttggtcggc gcagcggaag tgcaggtcgg cgttgcgggt cagcgcgccg
     2941 ggaagcaggt gcgcctcgca cagcacctgg aaaccgttgc agatacccag caccggcagc
     3001 ccctcgcgag cccctgcgat gacgagatcc atgaccgggg agaagcgggc gatcgcaccg
     3061 cagcgcagat agtcgccgta ggagaagccg ccgggaagca cgaccgcatc ggcgccgtgc
     3121 agcgaaggat ccttgtgcca cagggaaatc ggttcggccc cggccgcctt gatggcgcgc
     3181 agcgcgtcgc gatcatcgag agacccaggg aacgtgacga cgccgaccag cggtgccatc
     3241 agacatcgag cctgacgacg aaatcctcga tcacgggatt ggagagaaag ctcgcagcaa
     3301 gctcgtcgac ctgggcgagc gcagctgggt cggcctcgcc gtccaactcg atctcgaagt
     3361 gtttgccctg acgaaccgac gccacgcccg agcgaccgag cctgcccagg gcaccgagga
     3421 tcgcctgacc ctgcggatca aggatctccg gcttgagcat gacgtcgacg acgacgcgag
     3481 ccactggtgg cctccacagc gcgagggatc ggtgcgaact cacgtaccgc gatcctaccg
     3541 gcgcgcaccg acgcccaccg acgacctggt cggtctcccc tgatggcaca gttatgttgt
     3601 gaccgagacg ggcgccgagc gagcaagact caggcaagca gatggcggcg cggtcgactg
     3661 gcggcgctgg ggcacctacc tgtcggagcg cgcgtggggc agcgttcgcg aggattacag
     3721 cgcggacggc gatgcgtggg cgagtttccc cttcgagcac gccaggtcgc gcacctaccg
     3781 gtggaacgag gacggcctcg cgggcttctg cgacgtcgac cagacctggt gtctcggcct
     3841 tgcgatgtgg aactgcaagg acccgatcct gaaggagcgc ctgttcggcc tgacgaacag
     3901 tcagggcaac cacggcgaag acgtcaaaga gcggtactgg ttcgtcgacg gcacgcccac
     3961 acatacgtgg atgagctggc gataccacta cccgcagctg gcgtttcctt atgaccagct
     4021 gcgcaccgag aacgcccgac gaagccggct cgaccccgaa tacgagatcg acgacacggg
     4081 cgcgtttgcc ggcggcaggt acttcgatgt cggcgtcgac tatgcgaagg cggccgcgga
     4141 cgacctgtgc atgcggatca ccgtgcacaa cccgggagcg gaaactgcca cgatcgagct
     4201 gctcccgacg ctgtggtttc gcaacacctg gagctggggg ttgccgaagg cactgcccgc
     4261 accgaggatc acgtgcgacg gaccctcgct ggtcgccgtc cacgcaaagc tcggcacgct
     4321 gacactcacc ggcgcaccga ccgacttcgt cgaatcagtg gcctgcgaca acgagacgaa
     4381 cacgcggctg ctgtacgggg tgcccgggcc gccgtatccg aaggacggca tcggtgacca
     4441 cgtggtcagc ggcgccccga cagtgaaccc cgagggcttc gggacgaagg ccgcgctgcg
     4501 ctaccggctg gtgcttgcgc cgggggagac gcgcgaggtg cgggtgcgcc ttcaggccgg
     4561 gccgtcgacc gccgaccttg gagcaggttt cgacgcggtg ctgaccgcgc gcaagctgga
     4621 ggccgatggg ttttacgccg gtctgccggc gatggcgatg gcgtctgacg agccaccgga
     4681 tgtcacggcg ggcgaagcag cgatcgtgcg ccaggcaata gcaggattgc tgtggagcaa
     4741 gcagttttac cactatgacg tgcagcgatg gctcgacggc gaccccgctg gaccaccccc
     4801 accccttggt cgcggcgaca ttcgcaacgg caagtggacg cagctgaaca accacgatgt
     4861 gctcctcatg ccggacacgt gggagtaccc gtggtacgcg tcatgggacc tcgcttttca
     4921 ctgcgttgtg ctctcgcacg ccgaccctgc gtttgccaag cagcaacttc tgctgctgct
     4981 gcgcgagtgg tacatgcacc cgaacggcca gctcccggcg tacgagtgga acttctccga
     5041 cgtgaatccg ccagtgcatg cgtgggctgc actgcgggtg ttcgaggtgt ctgggggaac
     5101 cgattacgac ttcctcgagc gggcgtttca caagctgctg ctcaacttca cctggtgggt
     5161 gaaccaggag gaccacgacg gcaacaactt attccagggc ggctttctcg gcctcgacaa
     5221 catcggcccg ttcgatcgct ccaagctctc acccgacgcc ggggagctcg aacaaagcga
     5281 cggcacggcc tggatggcga tgtactgcct cgatctgctc gacatggctc tcgtgctcgc
     5341 cacgcacgac ccgacgtatg aagacgtcgc gaccaagttc ttcgagcact tcgcccacat
     5401 cgcgaccgct gcgcagcagc aaggcctgtg ggacgacggt gacggattct tctacgacca
     5461 gctccgcatg ccggacggcg agcggcggcc gatcaaggtc cgctcgctgg tcggcttgat
     5521 tgccgtctgc gcggtgagcc acgtcgacaa cgcgctgctc gcccaacttc cggggttcgc
     5581 cagccgcctc cagtggtttc ggacgaaccg accggccctg acgcattgcg tcgtcgcgac
     5641 ggaggacgac gacacgatgt tcgcgatgct ggatccggat cagctgcgca ggctgctctc
     5701 gcggatgttc gacgaggccg agttcttctc tccgcacggc atcaggtcga tctcggccta
     5761 ccaccgcgac catccgttcg agttctcgtt ggacggtcag gacttccggg tcgactacga
     5821 gccgggcgag tcctcgaccg gccttttcgg cggcaactcc aactggcgcg ggccggtgtg
     5881 gctccccttg aacgcgctgc ttgtcgaggc cctccagcgg ttcgaccagc acctcggcaa
     5941 ggacttcctg atcgaggtgc ccaagggctc tggccgcgaa atcactcttg gtcaggcggc
     6001 cgacgagctt gctggccggc tcatcagcct cctgctgcca gacagtgacg gacaccttgc
     6061 ggcgtccggc aatcgcgtct ggcccgaagg cctgctgtgg tttcacgagt acttccacgg
     6121 cgacaccggc gcggggctcg gcgcgtcgca ccagaccggg tggaccgcgc tgctcgccca
     6181 cctcgtgctt acccgccgcc actgaggctc atcggacgtc gatcacgttc agggggctcc
     6241 gacccccgag gacaggcgtg tgtgccactc ctcgggggtc tacgcctgtc ctcggcctgc
     6301 gcgggatccg cgagggcgtg tccacaggct gagacggttc tgtgaaatcg ctcagatttc
     6361 tgctgtccac atgcggtgga cgccggtagc cgaggccggg ggcgtggctg gaggatgcgg
     6421 gtcggcgcta tccacaggac ctgcacaccg gttccacagt tccaggcagg ttctgcacat
     6481 ggcgcgcaca accccctgtg cggcgcagac ttcccggcga ggacgcgagt gcctagggtc
     6541 ccgcatggcg gctgcgcggg ttggatgcga gcctgaaagt ttgcgcccag cagaaagtgt
     6601 cggcggtcgc cagtaccgat gtgatccatg agttccagat cggaaggcgc ggctgctgtg
     6661 agtgtcatcg agctccccgg atcggcgcgg gaattcgagc gcactccacc ccaagacatc
     6721 aacgctgagc aatcggtcct cggcggcatg ctgctcagca aagacgcgat tgccgatgtc
     6781 gtcgagtcgt tgcacggcac cgacttctac cggccggccc accagacgat cttcgacgtg
     6841 gtactcgacc tgtacggccg gggcgaacca gctgacgcgg tcaccgttgc ggccgagctc
     6901 accaagcgcg gcgagatcgc caggatcggc ggcgcgccct atctgcacac cctgatctcg
     6961 tcggtgccga cggccgcgaa cgcgggttac tacgcaaaga tcgtggccga gcgcgcgatc
     7021 ctgcggcgac tcgtcgaggc tggcacccgc atcgtgcaac tgggctactc gggcgttggc
     7081 aacgaggtag acgacatcgt cgaccgagcg caggcggcga tctacgacgt caccgagcgg
     7141 cggcagtctg aggactacag catcctcgaa tctctgctcc agccgaccat ggacgagatc
     7201 gaggcgagcg gtcatcgcgg cggcgggctg accggcgtcc cgaccggatt ctctgacctc
     7261 gactcgctga cgaacggcct gcaccccggc cagctgatcg tgatcgccgg caggccggcg
     7321 atgggcaagg cactcgccct tgagacgccg cttccgacgc cgttcgggtg gacggcgatg
     7381 ggtgaggtcg tggtgggcga ctggctgatg ggcgctgatg gtcggccgac gcaggtgatc
     7441 gcagcaaccg gtgtgctcct cgagcgtccg tgctatgagg tggagttcga cgatggttcc
     7501 gtcatcgtgg ccgacgccca gcaccaatgg ctcacggaga ctcgggccag ccgtcgctcg
     7561 ccgcgcacta tgaccggtcg ctcgtctcga tcggcagacc cgttcgcctt cggcgaggtg
     7621 cgcaccacgc aggagatcgc ggagacgctg cgatgcccaa cggcagacgc ccgaacaaat
     7681 cactcggttc caaatacgct gccacttcaa cttgacgaac aacagctgcc agtcccgccc
     7741 tacacactcg gggtctggct cggggttggg cacttcgcct cggcgcgctt caccagcgac
     7801 gacccagaga tcggctggca catcgaagcc gagggactag tggtgatccc ccgcggtggc
     7861 cggttgtacc gcctccgcct gccgagcgag agtgacgtgg atacccgcga atgcgctgtc
     7921 tgcggaaaga cgttcacgcc gaagacgatc gccgtacgaa cgtgctccca gtcgtgcggc
     7981 ggcaagtcgc gatccctggg cttaccgccg gccgcaaccc caacgtgcat gtcgtgtggg
     8041 gcgccgactg tcagcggttt cggcagatgc acgccctgcc gactcgatca cggctcggta
     8101 ggtgcgctac tccagcggat cggcgttctc aataacaaac acatcccggc cgagtacttg
     8161 cgcgcatcag aggaacaacg tcgggcactg ctcgccggcc tgcttgacac cgacggcacg
     8221 gtgtcaccga cgggcgccgt gcaaatcacg ctcaccgacg cgcggctcgc tcgcgacgtt
     8281 catgagctcg tcgtcagcct gggctaccga tgcggcttca cctccaaggc ggtcaacggt
     8341 catgccgcgg catccgcaac cgcctacacg acttctttca cggcagctga cagcccattt
     8401 cggctcgagc gcaagcgttt ggccctaaag gagcgcgcca ccaagcacac tcctcgaatc
     8461 ggtgcccggt tcatcgtcga tgtgcgtccg gtcccgtccg tgccggtcag gtgcgtcgag
     8521 gtcggcaacg cagcccactt gtatcttgcg ggttactcga tgatccctac tcataactca
     8581 acgctcggtc tcgacattgc gcgcgcggcg tcgatccggc acaaccagac atcggtcctg
     8641 ttctcccttg aaatgagcaa gcacgagatc acgatgcggt tgctgtcggc tgaggcgcgc
     8701 ataccattgc agaccatgcg cacgggccag atgcgcgaag acgact
//
LOCUS       DASWVF010000163         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_259472, whole genome shotgun sequence.
ACCESSION   DASWVF010000163 DASWVF010000000
VERSION     DASWVF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259472"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1352
                     /locus_tag="VGD55_02785"
     CDS             63..1352
                     /locus_tag="VGD55_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869300.1"
                     /translation="MVLLTADESAAPIDDGPIISERRRLLADVRPLRESPEYRRLWVG
                     QSLSSVGNQMTNVAVPVQVYAMTHSSLAVGSIGLAVGVPFIAVGLLGGSLADAVDRRK
                     LVIVTSSGLALLSFVFALQAALSLNQLWLLYVVVAVQSCLFAVDQPARSTFIPRLLPS
                     ERISAATALTQLSFQLSGTVGPLLAGVVIAEAGLKAAYFTDAATFTIAIYTVVRLRPM
                     PPGDGRAAPGIAAVAEGFRWVRHQPVIAMIFLVDLNAMIFGMPRAVFPALAATHFGGG
                     SRTVGLLYAAPAIGGLLGAAFSGPLAHVRRQGMAVLICVAIWGASIAGFSVSRSLVLA
                     AVLLAIAGAADMVSAVFRQTILLQNVPDALLGRLSALNFVVVAGGPRFGDLEAGGVAS
                     LTSPVFSAVSGGLACVVGVVALGLAVPAFARYRKPSQ"
     gene            1433..2500
                     /locus_tag="VGD55_02790"
     CDS             1433..2500
                     /locus_tag="VGD55_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0869301.1"
                     /translation="MRLPQRGVRARTDRGSLEPFDLFEILQKVPQCVIVTDADAAVVF
                     QNAAAWELDAVVRAQQGEPLLHALRATLTGRAWASGLLPVTVPVSVEMAVGQAHAELT
                     IDSIAGGFVASWTDHTAARERGRVVTRVATELASTADSFTSYADMLTSAVSEVSEQAH
                     AVASGSNQLTDSIQEIASSAATAVGNTAEAVRSAQSATSLVERLSESSDRIGAISRLI
                     STIAGQTKLLALNATIEAARAGAAGKGFAVVASEVKSLAQSSASATGDITSMITAIQV
                     DSAAAAAALEEIVHAISLMDSEQTTIASAVEQQSATAADIGARVAAVASAAQSSSTAL
                     IGLRAAADIVAAKSHELGRLV"
     gene            complement(2506..3573)
                     /gene="rsgA"
                     /locus_tag="VGD55_02795"
     CDS             complement(2506..3573)
                     /gene="rsgA"
                     /locus_tag="VGD55_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY0869302.1"
                     /translation="MSSSTMNNLGWDDARAAELASLESAHSQALTAARVSRVDRGALS
                     VLTVHGTDRVRMSGRLFQPGDHDEPIGTPAVGDWVAIEGSVAIAVLSRRSAFTRMSNA
                     GATVAQVVAANIDTVFVVAPLVGRVRPRLLQRCLALAWESGATPVVLLTKSDLAADVD
                     DRVAAAQAEAVGAQVHAVSASTGAGLDALEPYLIAGQTIAMIGPSGAGKSTLTNALGA
                     GTVALATGTVRDDGKGRHTTTARELVLLPSGAVAIDTPGLRGLALWDADEGIATAFAD
                     ITELAADCRFADCAHHGEPGCAVGQAIEQGDLDAARLADYAKLLREQRWLEIKRDTRA
                     RAQENARAKVSYRNMRRQPHR"
     gene            complement(3761..4531)
                     /locus_tag="VGD55_02800"
     CDS             complement(3761..4531)
                     /locus_tag="VGD55_02800"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY0869303.1"
                     /translation="MTQTTYGPNCSVDVRAPIMMHRWDTLTFLHWSFEPDVVARLLPE
                     GLTLETFEGKAWVGLIPFNMLVSLPHTPSVPWASRFCETNVRTYVHDKHGRPGIWFFS
                     LDASRLGAVVAARATFRLPYMWSHMSVRKDADIVTYNCTRRWPGPRGASSQVIVEIGR
                     PFEPGQLTARDHFLTSRWTLFSMTPNLTIGPTPGTKRVWQRLADAQHDPWPLQHATAL
                     EVDDQLVAAAGLPQPTSEPLVHYSPGVDVRIGRPRRLN"
     gene            complement(4575..>5066)
                     /locus_tag="VGD55_02805"
     CDS             complement(4575..>5066)
                     /locus_tag="VGD55_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869304.1"
                     /translation="EWVHVICGIGLPVPALIGLASKSFRKDAGLLNRFTRNDWKWLRS
                     RQRRDGSVVVGKFNAGQKLNASFQLGGILMMLSTGLVMRFANHWPVYLRTGATFVHDW
                     MAYAILAVVLGHLWMANRDPDALRGMRTGYVRTSWARREHEGWANREVARAKSSKASE
                     QST"
BASE COUNT          834 a         1625 c         1706 g          901 t
ORIGIN      
        1 gcagagatta accggctggc ctcaattcgg aggactcgtg gcggcgacga tcaccgacag
       61 ggttggttct gctgaccgct gacgagtccg ccgcacctat cgacgacggc cccataatat
      121 ctgaacgcag acgcctgtta gccgacgtac gtccactgcg cgagtcgccg gagtatcgcc
      181 ggttgtgggt cggccagtcg ctgtcgtcgg tcggcaatca gatgacgaac gtcgccgtgc
      241 cggtgcaggt ctacgcgatg acgcactcgt cattggctgt cggatcgatc ggcctcgccg
      301 tgggggtgcc gttcatcgcg gtcggccttc tcggtggctc gcttgccgac gcagtcgaca
      361 ggcgcaagtt ggtgatcgtg acgagcagcg ggctcgcgtt gctgtcgttc gtcttcgcgc
      421 ttcaggcagc tctgagcctc aaccaactct ggctgctcta cgtcgtcgtc gcggtgcagt
      481 catgtctgtt cgccgtcgac cagccggccc gttcgacctt cattccgcgt ctcttgccgt
      541 cggaacgcat ctcggcagcc accgcgctga cgcaactgtc gtttcagctg agcggcacgg
      601 tcggtccgtt gctggcgggc gtggtgatcg cggaggctgg actcaaggcc gcctacttta
      661 ccgacgccgc gacgttcacg atcgccatct acaccgtggt gcggttgcgg ccgatgccgc
      721 caggtgatgg ccgtgcggca ccgggtatcg ctgcggtggc agaaggtttc cgatgggtcc
      781 ggcaccagcc ggttattgcg atgatcttcc tcgtcgacct gaacgcgatg atcttcggaa
      841 tgccgcgtgc ggtgttcccg gcgctggctg cgacacattt cggcggtggc tcgcgaacgg
      901 tcggcctgct ctatgccgcg cccgcgatcg gcgggttgct cggagccgcg ttctccggcc
      961 ctctcgctca cgtcagacgg cagggcatgg ccgtgctcat ctgcgtcgcg atctggggcg
     1021 cctcgatcgc gggcttcagt gtgagccgat cgctggtgct tgccgccgtg ttgctcgcaa
     1081 tagcgggtgc cgccgatatg gtcagcgcgg tgttccgcca gacgatcctg cttcagaacg
     1141 tgcccgatgc cttgctcggc cggctcagcg ctctcaactt cgtggtggtt gcgggcggcc
     1201 ccaggttcgg tgacctcgag gctggcggcg tggcgtcgct caccagcccc gtgttcagcg
     1261 cggtcagcgg cgggttggcc tgtgtcgtcg gcgtcgtcgc gctcggcctg gcggtcccgg
     1321 cgttcgcccg ctaccgaaag ccatctcagt gagccttttg gctcagcgtg cccgcgcggc
     1381 tgccgatggg gcggcgagca ctaacgagcc gacggttgga ggacggactg ctatgcgatt
     1441 gcctcagcgc ggggtccgtg cccgcaccga ccgtggctcg ctggagccat ttgacctgtt
     1501 cgagatcctt cagaaggttc ctcagtgcgt catcgtcacc gacgcagacg ccgcagtcgt
     1561 ctttcagaac gcggcagcat gggagctcga cgcggtcgtc cgagctcaac agggcgaacc
     1621 gctgttgcac gcattgcggg cgaccctcac cggtcgagcg tgggcgagcg gactcttgcc
     1681 ggtgactgtg ccggtgtcgg tcgaaatggc agttgggcag gcgcacgccg agctcacgat
     1741 cgactcgatc gcaggtggct ttgtcgccag ctggaccgac cacactgcgg ctcgcgaacg
     1801 cgggcgggtg gtgacccggg tggcgaccga acttgcaagc accgctgatt cgttcacctc
     1861 ctacgccgac atgctgacga gtgccgtgag tgaggtttcc gagcaggcgc atgcggtggc
     1921 gagcggatcg aaccaactga ccgacagcat ccaagagatc gcgagcagcg ctgctactgc
     1981 ggtcggcaac accgcagaag ccgtgcggtc ggcgcagtcg gcgacctcgc tcgtggaacg
     2041 gttgagtgag tcgagtgacc gcatcggagc gatcagccgc ctcataagca cgatcgccgg
     2101 tcagacgaag ttgctcgcgc tgaacgccac tatcgaggct gctagggctg gcgcagcagg
     2161 caagggattc gccgtcgtcg ccagcgaagt gaagagtctg gctcagagca gcgcatcggc
     2221 taccggtgac atcaccagca tgatcaccgc gatccaggtt gacagtgcag cagctgcagc
     2281 tgcactcgaa gagatcgtgc acgcgatctc gctgatggac tccgagcaga caacgatcgc
     2341 cagcgccgtc gagcagcaaa gcgctaccgc cgccgacatc ggagcacgag tagccgcggt
     2401 ggccagtgca gcgcagtcgt catcgacagc actcatcggg ttgcgcgcgg cagccgacat
     2461 cgttgctgcc aagtcgcacg aactcggccg gctggtgtag tcgacctagc ggtgcggttg
     2521 gcggcgcatg tttctgtaag acaccttggc ccgcgcgttc tcttgggcgc gcgctcgggt
     2581 gtcgcgtttg atttctagcc aacgctgctc gcgcagcaac ttcgcgtaat cggccagcct
     2641 cgcggcgtcc agatcgcctt gctctatcgc ctgaccgacc gcgcagccgg gctccccgtg
     2701 atgagcgcaa tcggcgaagc ggcaatcggc agcaagctcg gtgatgtcgg cgaacgcggt
     2761 ggcgatgccc tcgtcggcgt cccacaaagc gagaccacga agccccggcg tgtcgatcgc
     2821 gactgcacca gacggcagga gcacgagttc gcgggccgtc gtcgtgtgcc gacccttccc
     2881 gtcgtcgcgc acggtccccg tcgccagcgc aaccgtgccg gcacccaacg cattggtgag
     2941 cgtcgacttc ccggcgcccg acggaccgat catcgcgatc gtctgccccg ctatcaggta
     3001 cggctcgagc gcatcgagcc cggcgcccgt tgatgcgctg acggcgtgta cttgcgcacc
     3061 gacggcttcc gcttgggcgg cagcaacgcg gtcatcgacg tcggccgcga gatctgactt
     3121 ggtcaacaag acgaccggcg tcgcacccga ttcccaggcc agcgctaggc accgttgcag
     3181 taaccgcggt cgcacccgac ctacgagagg cgcgacgacg aacacggtgt cgatgttcgc
     3241 tgcgacgact tgggcgacgg ttgcgccggc attgctcatg cgggtgaacg cagaacgacg
     3301 cgagaggacg gcgatcgcga ccgacccctc gatggcgacc caatcaccca ccgccggagt
     3361 gccgatgggt tcgtcgtgat cgcccggctg gaacagccga cctgacatcc gcactcgatc
     3421 cgttccgtgc acagtcagca cgctgagcgc gccccggtcg actcgcgaga cgcgggctgc
     3481 cgtaagagct tggctgtgcg cggattcgag cgaggcaagt tcggctgcac gcgcgtcgtc
     3541 ccagccgaga ttgttcatag ttgatgaaga catgatcgag cgctcctaca cgtgaaggtg
     3601 ggcgctcggc gcagcggttc aggtgttgga cagcaccgca gttagaaggc accgagcggg
     3661 aggacggcgg gcatgacggc aacgtcagtc atggaagcca cctcctctcg actcgagttg
     3721 tctgcagggt aggccggaag ggcgacgcgg cgctaacggt ttagttgagc cgccgaggcc
     3781 gaccgatgcg gacgtcgacc cctggcgagt agtgcaccag cggctcactc gtcggttggg
     3841 gtaaccctgc agccgcgacc agctgatcgt cgacttcgag cgccgtcgcg tgctgcaacg
     3901 gccacggatc gtgctgcgca tcggcgaggc gctgccagac gcgtttcgtg ccgggcgtag
     3961 ggccgatggt caggttcggc gtcatgctga acagggtcca gcgagaggtg aggaagtggt
     4021 cgcgcgctgt cagctgacca ggctcgaatg gccggccgat ctcgacaatc acctggctcg
     4081 atgcaccgcg cgggcccggc cacctgcggg tgcagttgta cgtgacaatg tcggcgtcct
     4141 tgcgcacaga catgtgcgac cacatgtacg gcaaccgaaa cgtcgcacgg gccgccacca
     4201 ccgcaccgag ccgcgacgcg tcgagggaga agaaccagat gccagggcgg ccgtgcttgt
     4261 cgtgcacgta ggtgcgcacg tttgtttcgc agaatcgcga tgcccacggc actgacgggg
     4321 tgtgcgggag gcttaccagc atgttgaagg gaatcagccc gacccaggct tttccctcga
     4381 acgtctcgag cgtgagccct tcgggtaaca gtcgcgccac gacgtccggc tcgaaagacc
     4441 aatgcaggaa cgtcagcgtg tcccaccggt gcatcatgat cggcgcccgc acgtcgacgg
     4501 agcagttcgg tccgtacgta gtttgggtca cgagagccat cctgccggac gccgatgagc
     4561 cggcgattat tgcctcaggt gctctgctct gaggctttgg aggactttgc tcgcgccacc
     4621 tcgcggttgg cccagccctc gtgctcgcgg cgggcccacg aggttcggac gtagccggtg
     4681 cgcatgccgc gcaacgcgtc gggatcgcgg ttggccatcc agaggtgacc gagcaccacg
     4741 gccaggattg cgtaggccat ccagtcgtgt acgaacgtcg cgccggtgcg caggtagacc
     4801 ggccagtggt tcgcgaaacg catgaccaag ccggtgctga gcatcatcag aatgccgcca
     4861 agctggaaac tggcattgag cttttggccc gcgttgaact tgcctaccac gacggagccg
     4921 tctcgacgct ggcgcgagcg cagccacttc cagtcgttgc gggtgaaccg gttgagcagc
     4981 ccggcatctt tgcggaacga cttcgacgcg aggccgatca gggcgggcac gggcagaccg
     5041 ataccgcaga tgacgtggac ccattc
//
LOCUS       DASWVF010000164         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_437732, whole genome shotgun sequence.
ACCESSION   DASWVF010000164 DASWVF010000000
VERSION     DASWVF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437732"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VGD55_02810"
     CDS             <1..1534
                     /locus_tag="VGD55_02810"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0869305.1"
                     /translation="TREVLALKGQTPEAMQLRLAVLEQQLQRAQKAIFGASSEKRPHD
                     GADNEGDKPSPEKKPRKGHGPTKQPELLNIDVTHALDKADQVCTQCGGALEEWDGQCE
                     EHEEVDVVARRFIIKRHKRKKYRCSCGACVETAIGPEKLTEGGRYSVDFAIEVATQKY
                     CDHAPLERQVRSMKRDGLDVTSQTLWDQINALARILSPALPRVLSHVLGYGVIGVDET
                     RWKLLGDKSCGGGSKTWQVWAVSTPTAAYYQIEDSRSGDAAEKILGGYTGTVMCDGYG
                     VYAALLKRPACKDLRLAHCMAHVRRAFCEVEAFFPEQCGQVLELIGQLYAIDAECPAG
                     PAGDDTRRRLRDTKSRDVVGRMMAWVYATVPTILPESGLMKAIKYMAGVWTGLVRFLD
                     EPRIPLDNNACERALRGPVVGRKNHYGSRSQRGTEVAALFYTLVESAKLNDLDPRLYL
                     RIAARAGLRGETIPLPHEVARPPENDRVRRERVDNETERLVAAAILANPRKDDEDARR
                     DV"
BASE COUNT          353 a          610 c          666 g          296 t
ORIGIN      
        1 gacgcgcgag gttctcgcgc tcaagggcca aacgccggag gcgatgcagc tccggttggc
       61 ggtgctcgag cagcaactgc agcgcgcgca gaaagccatc ttcggtgcgt cctccgagaa
      121 gcgcccgcac gacggtgctg acaacgaggg cgacaagccg tcgccggaga agaaaccgcg
      181 caaggggcat ggcccgacca agcagcccga gctcctcaac atcgacgtca cgcacgcgtt
      241 ggacaaggcc gaccaggtct gcacgcagtg cggtggagcg ctcgaggaat gggacgggca
      301 gtgcgaggag cacgaggagg tcgacgtcgt cgcgcgccgc ttcatcatca agcggcacaa
      361 gcgaaagaag tatcgatgta gctgcggcgc ctgcgtcgag accgcgatcg gcccggagaa
      421 gctgacggag ggtggacggt actcggtcga cttcgcgatc gaggtggcga cgcagaagta
      481 ctgtgaccac gcacccctcg agcgccaggt gcgcagcatg aagcgcgacg gtctcgacgt
      541 gacgtcacag acgctgtggg atcagatcaa cgcactcgcg agaatccttt cgcctgcact
      601 gcctcgggta ctgtcccacg tcctcgggta cggcgtgatt ggcgtcgacg agacgcgctg
      661 gaagctgctc ggcgacaaga gttgcggtgg tgggagcaag acgtggcagg tatgggccgt
      721 gtcaacgccc accgcagcgt actaccagat cgaggacagt cgctcgggtg acgcggccga
      781 gaagatcctc ggcggttaca cgggaactgt gatgtgcgac gggtatgggg tgtacgccgc
      841 gctgttgaag cgccccgcgt gcaaggacct gcgcctcgcc cattgcatgg cccacgtgcg
      901 tcgtgcgttc tgcgaggtcg aggcgttctt cccggagcag tgcgggcagg tcctcgagct
      961 catcgggcag ctctacgcga tcgatgccga gtgtccggct gggcccgcgg gggacgacac
     1021 gcggcgacgc ctgcgtgaca ccaagtcccg tgacgtcgtg ggacggatga tggcgtgggt
     1081 atacgcgacg gtgccgacga ttctgcccga gtcgggactg atgaaggcca tcaagtacat
     1141 ggcaggagtc tggacgggcc tcgtgcgatt cctcgacgaa ccgagaatcc cgctcgacaa
     1201 caacgcgtgc gagcgagcgc ttcgcggacc cgtcgtgggt cgcaagaatc actatggctc
     1261 ccgctcgcaa cgcggcaccg aagtcgccgc gctgttctac acgttggtcg aaagcgcgaa
     1321 gctgaacgac ctcgaccctc gcctctatct gcgcatcgcc gcgcgcgctg gccttcgagg
     1381 ggagacgatc ccgctgcctc acgaggtcgc gaggccccct gagaatgatc gcgttcgacg
     1441 cgaacgcgtc gacaacgaaa ccgagcggct cgtggccgct gccatactgg cgaacccccg
     1501 caaggatgac gaggacgccc gccgcgacgt ctgactcgcg ccgccggtga cgagcgccgt
     1561 tgcgtgttgc gtgcgcgcgg cggctggctg ttggcacgca acgcgtagtc acagttgccg
     1621 catccgatcg aggtcgccgc cgcggggacg cggcggcccg tcacgcgcca acatcgaaat
     1681 tgtcgcgatg tcgccgagcg tcgcaggcgc ggcaggtcac ggccaccaac tcggagcgcg
     1741 ctacgcgcgt ccgagagacc tccgcccgac cgggtgctcg cgcgcgcgta ctatgcctgt
     1801 ttcggggcga gccgacgggc cacggcgagg acttacaatt cagtcgtgga aatctgagcg
     1861 caccgcccgc cagttacata cgacggttgc gtagtctaca gttggatctt ccgcagttct
     1921 ggctc
//
LOCUS       DASWVF010000165         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_361193, whole genome shotgun sequence.
ACCESSION   DASWVF010000165 DASWVF010000000
VERSION     DASWVF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361193"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..999
                     /locus_tag="VGD55_02815"
     CDS             157..999
                     /locus_tag="VGD55_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEY0869306.1"
                     /translation="MASVDGAVAVRARRRPLGRYGLRFVALGYLLVLLAAPVAMVFYR
                     AFQHGFGPIWSALTTSEARSAIELTLTVAIAAVVLNTIFGVGTALLLVRRRFPGRHLL
                     DVLVDIPQAVSPIVVGLALILVYGKFGFFGSWIERNGLKIIFSVPGMVLATAFVSLPL
                     VVRSVAPVLTELGDEQEQAARTLGASSWQTFRRITFPSIRDALAYGVVLSLARSLGEY
                     GAVTVVSGHVIGKTLTLTLFIDERLQNFDQPAAYSAAVLLAIIAMGSLLISRRFRSRS
                     RSTT"
     gene            996..2006
                     /locus_tag="VGD55_02820"
     CDS             996..2006
                     /locus_tag="VGD55_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY0869307.1"
                     /translation="MSISVRHVTKRFAQFTALDNVSVDIPTGSLTALLGPSGSGKSTL
                     LRVIAGLDTADQGTVSIEGTDATNLPPQRREVGFVFQHYAPFKHMTVYDNIAFGLRVR
                     KASKAETKSKVTELLELVHLEKFQTRYPAQLSGGQRQRMALARALAVEPRVLLLDEPF
                     GALDAQVRKELRTWLRRLHDEMHVTTVIVTHDQEEAMEVADEIVVLHDGRVQQVGHPH
                     DVYEHPANDWVMGFLGPVTHFGDLLVRPHDLDLAHEPSDGALPATVVRVTRLGFQTVV
                     EMDADGAPIYAQLAREATDRLTLEPGAEVHVRHARGEALPGAPAIPALGPELQTAEAP
                     LK"
     gene            complement(2168..2626)
                     /locus_tag="VGD55_02825"
     CDS             complement(2168..2626)
                     /locus_tag="VGD55_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0869308.1"
                     /translation="MHISAKTDYAMRAMLVLADAQRDTWVKAETIAGEQHLPRKFLEA
                     ILSDLRRARLVESLRGADGGYRLARPASKIQVADVMRAVDGPLAEVRGLRPETLEYEG
                     SAERLQDVWVAVRAGLRAVLERISVADVAAGKMPASVRGMIRDLEAWVPR"
     gene            complement(2678..>3369)
                     /locus_tag="VGD55_02830"
     CDS             complement(2678..>3369)
                     /locus_tag="VGD55_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869309.1"
                     /translation="AFEDIEGWGANMAGFRTGRRLVWIPLVIAPGATTLTGCKKTIHA
                     VKPLTNISQAPTASSSAAATPASVSPASATANTPSAVASLSATAVPVATPSAKAGAGC
                     NLVPLAAVASALGVASITSSTEAVTTLRAPIIAHQGCRYVAGAAGAGWDLNTFSAPPP
                     PALIAAEAAKIPGAKATTIDGLPAFTAPIPGVTPEEEVSVYKGSQSLVIISHGKTGAS
                     VAIAQLILAAI"
BASE COUNT          561 a         1077 c         1121 g          610 t
ORIGIN      
        1 accttccctt ccacacggaa gtctctgcgg tgaatatcta cgaccagatt caaagcgaca
       61 atcttgtcgg cgctgctgcc cagtcgaccg ttcttctcgt cgtcgcgttc gtggtgctgg
      121 tcgcgctgga tcagctcaag cgttggggtg tgcggcgtgg ctagtgtcga cggcgccgta
      181 gcggtgcgcg ccaggcgacg ccccctcggt cgctatggcc tgcggttcgt tgccctcggg
      241 tatttgctcg tgctgcttgc agcgcccgtc gcgatggtgt tctaccgcgc gttccagcac
      301 ggcttcggcc cgatctggtc agcactgacg accagcgagg cgcgcagtgc catcgagctc
      361 acgctgacag tcgcgatagc agcggtcgtg ctcaacacga tcttcggcgt cgggacggcg
      421 ctgttgctcg tgcgtcgccg attccccggt cgtcacctgc tcgacgtcct ggtcgatatt
      481 ccgcaagctg tgtcgccgat tgtggtcggc ctcgcgttga ttctggtcta cggaaagttc
      541 ggcttcttcg gatcatggat cgagcggaac gggctgaaga ttatcttcag cgtgcccgga
      601 atggtgttgg cgaccgcgtt cgtatcgctg cccctcgtcg tccgatctgt tgcacctgtc
      661 ctgaccgaac ttggcgacga gcaagagcag gccgcgcgca ccctcggtgc atcgagttgg
      721 cagacgtttc gacgcattac gttcccgtcg atccgcgacg cgcttgccta cggcgtcgtg
      781 ctctcgcttg cgcggtcgct cggcgagtat ggcgcggtga ccgtggtatc gggccatgtg
      841 atcgggaaga cgttgaccct gaccctgttc atcgacgagc ggctgcagaa ctttgaccag
      901 ccggcggcct actcggcggc ggttttgctt gcgattattg cgatgggaag cttgctgatc
      961 agcaggcggt tccgcagccg aagccggagc acgacatgag catctcagtg cggcacgtga
     1021 cgaagaggtt cgcgcagttc acggccctcg acaatgtcag cgtcgatatc ccgacgggct
     1081 cgctgaccgc attgcttggg cccagtggca gcggcaagtc gacgttgctg cgcgtgatcg
     1141 ccggcctcga tactgccgac cagggcacgg tgagcatcga aggaaccgac gcgacgaacc
     1201 tgccgccaca acggcgcgag gtcgggttcg tctttcagca ttacgcaccg ttcaagcaca
     1261 tgaccgtcta cgacaacatc gcgttcgggc tgcgagtgcg caaagctagc aaggcagaga
     1321 cgaagagcaa ggtgaccgag ttgctcgagc tcgtgcacct ggagaagttt cagacccggt
     1381 atccggcaca gctttcgggt ggtcagcggc agcgcatggc gttggcgcgc gcgctcgccg
     1441 tcgagcctcg ggtgttgctg ctcgacgagc cgtttggcgc gctcgatgct caggtgcgca
     1501 aggagttgcg cacctggctg cggcgcctgc acgacgagat gcatgtgaca actgtcatcg
     1561 tcacccacga tcaggaagag gcgatggaag tcgccgacga gatcgtcgtg ctgcacgacg
     1621 gcagggtgca gcaggtcgga catccgcacg acgtctacga gcatccggcg aacgattggg
     1681 tcatggggtt ccttggcccg gttacgcact tcggagactt gctggtgcga ccgcacgatc
     1741 tcgatctggc tcatgagccg tcagacggcg cattgccggc gactgtcgtg cgcgtcacgc
     1801 gcctcggttt ccagacagtc gtcgagatgg acgccgacgg cgcgccgatc tacgcacaac
     1861 tcgcgcgcga agctacggat cgactgacac tcgagcccgg ggccgaggtg catgtgcggc
     1921 acgcgcgggg cgaggcgctc cctggtgcgc ctgccattcc cgccctaggg cccgaactgc
     1981 aaaccgctga agccccactc aagtagtcgg tcgccggccc atcgccgcac aagagtccgc
     2041 tcgtcgcgct gcgctcatgc gaacacctcg acgctgccgc ccggtgacgg tgaggcgcgg
     2101 gttccggtac ccgagttgga agcttccgga cgctcaagag agcgtccaga gggaaccaag
     2161 tgtcggtcta gcggggtacc caggcttcga gatcgcggat catgccgcga actgaggcag
     2221 gcatcttgcc cgcggcgacg tcggcgacgc tgatccgttc gagcaccgca cgaagtccgg
     2281 cgcggacggc gacccacacg tcttgcagcc gctcggcgga accttcgtac tcgagagtct
     2341 cagggcgcag gccacggacc tcggcgagcg gtccgtcgac ggctcgcatg acgtcggcaa
     2401 cctggatctt cgaggccggc cgggcgaggc ggtatccgcc gtcagcacca cgaaggctct
     2461 cgaccagccg cgcgcggcgt agatcggaca ggatcgcctc aaggaacttg cgcggcagat
     2521 gctgctcacc ggcgatcgtc tcggccttca cccaggtatc gcgctgcgcg tcggcgagaa
     2581 cgagcatggc tcgcatcgcg tagtcggtct tcgccgagat atgcacggcc ccatgatgac
     2641 agggccatcg ccgttcgagg acgcgccgcg accaacctca gatcgctgcc agtatcagtt
     2701 gggcgatagc gaccgaggcg ccggtcttac cgtgcgaaat gatgaccagc gactgagacc
     2761 ctttgtacac ggacacctct tcctcaggcg ttacacccgg aatcggggca gtgaacgcgg
     2821 gcagaccgtc gatggtcgtc gctttggcgc ccgggatctt cgccgcctcg gctgcgatca
     2881 gcgccggcgg cggcggggcg ctaaaggtgt tgaggtccca tcccgcgccg gccgcgcccg
     2941 cgacgtaccg gcaaccctgg tgggcgatga tcggcgcccg caacgtcgtc accgcttcgg
     3001 tcgaggatgt gatcgatgcg acgccgaggg cgctcgctac cgcggccaac gggaccagat
     3061 tgcaacccgc gccagccttt gcagacggcg tagcaacagg aacggcggtc gccgacaagc
     3121 tagcgactgc tgatggcgtg ttcgccgtgg ccgaggccgg gctgaccgag gcgggcgtgg
     3181 cggccgcgct gcttgaagcg gtcggggcct gagaaatgtt ggtgagcggc ttgaccgcat
     3241 gaatggtctt cttgcagccc gtaagggtcg tcgcgccggg cgcgatcacc aaggggatcc
     3301 agaccagccg tcgccccgta cgaaacccag ccatgttcgc gccccaacct tcgatgtcct
     3361 cgaaggcta
//
LOCUS       DASWVF010000166         4973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_106639, whole genome shotgun sequence.
ACCESSION   DASWVF010000166 DASWVF010000000
VERSION     DASWVF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106639"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1212)
                     /locus_tag="VGD55_02835"
     CDS             complement(118..1212)
                     /locus_tag="VGD55_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0869310.1"
                     /translation="MDVVEPYPPEPSLSAPASPSSTLSLSSSPPPSPPPSPSYARSRQ
                     FARGNRRGLLAVGSLLVLSVVMATIFGVTQMDRLNTTRHQVSTLLASQAAQQAQVGAL
                     TTTVSAQAKTLATLDSELKSAQAQLTTTTSQLSTTQTQLQTAQTRLGADEQQLNTTTR
                     QLPPDLVALATQVSPSVVLITCTTGTGGDTGTGFAISLPAASGYATTIITAEHVIDAC
                     TDPASGAFISLARGSKTLQVHLRSYDPGEDVAVLDTTATLPKLKPASSPVVGEFLMAV
                     GNPLGVSDNVTSGNVSQVYSTYFLDSAPISNGSSGGPVVDRSGNVVGIVDAGAVADAN
                     SPVVQNLNVALRLSRLCVNLLGGSSCSALH"
     gene            complement(1308..2621)
                     /locus_tag="VGD55_02840"
     CDS             complement(1308..2621)
                     /locus_tag="VGD55_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869311.1"
                     /translation="MRTAALASMLAALVATLAGCASVGSARTQGSGSPGSSAVAVFTP
                     TPGEPLKLVGSWLLDAPGEPLGTVLRLGDDLSIWSDCGELMGDWAANPAGLFAAQVAG
                     WSGSCVASTSTNDPTPTWLKRVVGFKADAAREQLLDATGAVVATLRPGGQPTAGPDIS
                     ADLTKPPTVTDSLRARLRGGQPLPAGLVAARPAELVGRWVWDARPIPRGFAQLAFDGS
                     WTGSDGANGQGGRWSAALDGELVVVAGAQTLAGCGDPAACADVGGSFGSAARAAFDGS
                     TLVLLDADGKVTGRLVRAPAVQAVAPASSAGPQSPAPTSALASDTTASSSAASSDVSI
                     ACRKSLGASVFASGLIAVADIRAYTLGPGPYTPAPSAFPGLSDDATVAWCWTSQPEPT
                     PSPQDPTLYVWWVVGPDGSPIRMAGIGGSPGVINPGVFNGWPAIP"
     gene            complement(2731..3291)
                     /locus_tag="VGD55_02845"
     CDS             complement(2731..3291)
                     /locus_tag="VGD55_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0869312.1"
                     /translation="MRTLIVTEFISLDGVVEAPGGEGTHPHGGWTMAYGTSEEFEYKL
                     KETMDAESMLLGRITYQGFAEAWPTMQDVFADKMNSMPKHVVTNTLTDLEWNATPISG
                     DVVAGVTELKNGDGGPILVHGSATLVQFLLAEHLVDELHLMTFPVVIGGGLRMFPDDR
                     AKLEFELVELARYDTGVLLQVYRPVA"
     gene            3439..3738
                     /locus_tag="VGD55_02850"
     CDS             3439..3738
                     /locus_tag="VGD55_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0869313.1"
                     /translation="MFALVVRFDLPDAAAADAFDELTAQAVPLIRANEPGTLVYEPHA
                     IEGEPLSRVFYEVYRDREAHAEHERQPHTMQCLAGVRALVTTTRVELLTPQLPTP"
     gene            complement(3818..4582)
                     /locus_tag="VGD55_02855"
     CDS             complement(3818..4582)
                     /locus_tag="VGD55_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869314.1"
                     /translation="MNARPYRAAAVAAAVAVAFGLSGCAAINKVKTVAHDIKGNKATI
                     DSFTTKIQSGEAKPFQATYVTTGDTPTTVVYAVQPPTDVSFTNTPSTLTSPGTSASAP
                     NIDIIVNASGDYSCTPPDTSGSAGTGVWACQKLSSDAAATQNAIVDFYTPAHWINFLK
                     GFSIAAGFAGDQVTSSTMSVNGFDMSCVDFKTPGDADTSTICTTSQGILGYVKVAGDT
                     TSFEIKSYSATPDASLFALPPGAKVTDVQLPSSPAS"
     gene            4783..4944
                     /locus_tag="VGD55_02860"
     CDS             4783..4944
                     /locus_tag="VGD55_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869315.1"
                     /translation="MEGANPDAVTVEALARLQLIARRNGCTLRLRNASAELLDVIELM
                     GLGSVLTQS"
BASE COUNT          836 a         1646 c         1668 g          823 t
ORIGIN      
        1 acccgctcga agtagctgct cacaccccgg cgaacatcgt ggagtttcga catacctcta
       61 gcaacgtcgg cactccacaa tcaccgacgg ctcgatcgcc gcgcacggtc gtaaggatta
      121 gtgaagcgcc gagcacgacg agccgccgag caggttgacg cacagcctcg acaggcgcaa
      181 cgcgacgttg aggttctgca cgaccggtga gttggcgtcg gcgaccgcgc cggcgtcgac
      241 gatgccgacc acgttgccgg atcgatcgac gaccggccca cctgagcttc cattgctgat
      301 cggtgcgctg tcgaggaagt acgtcgagta gacctgcgac acgttgcccg acgtcacgtt
      361 gtcgctgact cccaacgggt tgcccacggc catcaggaac tctccgacca ccggagacga
      421 tgcgggcttg agtttcggca gcgtcgcggt cgtgtccagc accgcgacgt cctcgcctgg
      481 atcgtaagaa cgcaggtgca cttgaagcgt cttgctgccg cgtgccagcg agatgaaggc
      541 gccgctcgcg ggatcggtgc acgcgtcgat gacgtgctcg gcggtgatga tcgtcgtcgc
      601 gtagcccgac gccgccggca acgagatcgc aaacccggtg ccggtgtcgc cgcccgtccc
      661 cgtcgtgcaa gtgatgagca ccaccgacgg cgagacctgg gtcgcgagcg caacgaggtc
      721 gggcggcagt tggcgggtcg tggtgttcag ctgctgttcg tcggcgccga gcctggtctg
      781 tgcagtctgg agttgggttt gtgtcgtgct cagctggctc gtggtggtgg tcagctgcgc
      841 ttgcgccgac ttcagctcgc tgtccagcgt cgcgagtgtc ttcgcttgcg cgctaaccgt
      901 cgtcgtcaac gccccgacct gcgcttgctg cgcggcttgc gatgcgagca acgtcgacac
      961 ctgatggcga gtggtgttga gccggtccat ctgcgtcacg ccgaagatcg tggccatgac
     1021 gacggaaaga acgagcagcg atccgacggc gaggagtccg cgcctgttac cgcgggcgaa
     1081 ctgccgcgac cgcgcataag agggggacgg cggcggggac ggcggcggcg aggaagacag
     1141 cgaaagcgtg gacgaggggg acgccggcgc agacagcgag ggttctggcg ggtagggctc
     1201 taccacgtcc atctgcgccc ccgcgatcgt cggctagcaa ccaatgtccg ttctaccgca
     1261 ccgagccttc gagcagtaat ccgcgaagag aaactccacc accgttgcta ggggatcgcc
     1321 ggccacccgt tgaacacacc tggattgatg actcctgggc taccaccgat acccgccatc
     1381 cggatcggcg agccgtccgg accgactacc caccagacgt agagcgtcgg atcctgcggg
     1441 ctcggcgtcg gctcgggttg cgacgtccag caccacgcga cagtcgcgtc gtcactcagc
     1501 ccgggaaacg ccgacggcgc tggcgtgtac gggccgggac caagcgtgta tgcacggatg
     1561 tcggccacgg cgatgaggcc cgacgcgaag acgctcgcac ccagcgactt tcggcacgca
     1621 atcgacacgt cggatgatgc cgcggacgaa ctggcggtcg tgtccgacgc gagagccgaa
     1681 gtcggagcgg gcgactgcgg accagcggag ctcgcgggcg cgactgcctg gaccgcaggc
     1741 gctcgcacga gacggccggt gaccttcccg tcggcgtcca gcaataccaa tgtcgaaccg
     1801 tcgaacgcag ctcgggccgc cgagccgaac gaaccgccga cgtcggcgca cgctgcggga
     1861 tccccgcagc cggccaacgt ctgggctccc gcgacgacga cgagctcgcc gtcgagcgcc
     1921 gctgaccagc ggccgccctg gccgttggcg ccgtccgaac cggtccacga cccgtcgaac
     1981 gcaagttggg cgaagccgcg cggtatcggt ctcgcatccc aaacccatcg gccgacgagt
     2041 tcagcagggc gggccgcgac gaggcccgcc ggaagcggtt gcccaccgcg cagccgcgcg
     2101 cgcagcgagt cggtgaccgt cggtggcttc gtgagatcag cagagatgtc agggccagcc
     2161 gtcggctgcc cacccggacg cagggtcgcc accactgcgc cggtcgcgtc gaggagttgc
     2221 tcacgtgcgg cgtctgcctt gaagccgacg actcgcttga gccacgtcgg cgtcggatcg
     2281 ttcgtcgacg tgctggcgac gcacgagccg ctccaacccg cgacctgcgc ggcgaagagt
     2341 ccggcagggt tggcggccca gtcgcccatc agctcgccgc agtcgctcca gatcgagagg
     2401 tcgtcgccaa gtcgcagcac cgtacccaat ggctcgccgg gagcgtcgag caaccacgaa
     2461 ccaacgagct tcagcggctc acctggcgta ggcgtgaaca ccgcgacggc gctgctgccc
     2521 gggctcccgc tgccctgcgt ccgcgcgctt ccaacgctcg cgcaacctgc caatgtggct
     2581 actagcgcgg caagcatgga agcgagcgcg gcggtacgca tcgacgccgg cgatcctcgc
     2641 ctgaccattg cgcctccccg caagcatcat gcagcaatac cgcttcgacg cgtcaggatc
     2701 gcggcacgtt ccactcacgt aggcaggcgc tcaagcgacc ggccggtaca cctggagcaa
     2761 gacgccagtg tcgtagcgcg ccaactcgac gagttcgaac tcgagcttgg ctcggtcgtc
     2821 ggggaacatc cggaggccac cgccgatgac cacagggaac gtcatgagat gcaactcgtc
     2881 gacgaggtgc tccgcgagga ggaactgcac gagggtcgcg ctgccgtgca ccaggatcgg
     2941 accaccgtcg ccgttcttga gctcggtgac gccggcgacg acgtcgccgc tgatcggggt
     3001 cgcgttccac tcgaggtcgg tcagcgtgtt cgtgacgacg tgcttgggca tcgagttcat
     3061 cttgtcggcg aagacgtctt gcatcgtcgg ccacgcctcg gcgaacccct ggtaggtgat
     3121 gcggccgagc aacatcgact cggcgtccat cgtctctttc agtttgtact cgaattcttc
     3181 agaagtgccg taggccatcg tccagcctcc gtgcggatga gtgccctcac cgcctggtgc
     3241 ttcgacgaca ccgtcgagac tgatgaactc ggtgacaatc aaggtgcgca tgcggccctc
     3301 ccgtttgtag tgtcagtgat tggacgccgc gcggctggcg aactcatcgg ttcatggatc
     3361 agccggctca agaggtggtc gatcacattc ggccacccta gggcgccggg ccggatcgcc
     3421 accgctagcg tggttcgggt gttcgccttg gtggtcaggt tcgacctgcc cgacgcggca
     3481 gctgccgacg ccttcgatga gctgaccgcc caggccgtgc cgctgatccg cgcgaacgag
     3541 cccggcacgc tggtctacga gccgcacgcg atcgagggcg agccactttc gcgggttttc
     3601 tacgaggtct accgcgaccg ggaagcccac gccgaacacg aacggcagcc gcacacgatg
     3661 cagtgcctgg caggagtgcg tgcgctggtc accacgacgc gggtcgagtt actgaccccg
     3721 caactaccaa cgccctgaac cgcccgccgt ccggcccgaa catcgtggag tctggtggta
     3781 cctagagcaa ccgtggcgct ccacgttaat gcggtgctta gctcgcggga gacgacggca
     3841 gctgtacgtc ggtgaccttg gcgcccggtg gcaacgcgaa gagcgacgcg tcgggtgtcg
     3901 cgctgtagct cttgatctcg aagctggtgg tgtcgccggc caccttcacg tagccgagga
     3961 tcccctgcga ggtcgtgcag atcgtgctcg tgtcggcgtc gccgggtgtt ttgaagtcga
     4021 cgcacgacat gtcgaacccg ttgacgctca tcgtcgacga cgtcacctgg tcaccggcaa
     4081 acccggccgc gatcgagaag cccttcagga agttgatcca gtgcgcgggg gtgtagaagt
     4141 cgacgatcgc gttctgcgtc gcggccgcgt cggatgacag tttctggcac gcccagacgc
     4201 cggtgccggc cgaacccgag gtgtcgggcg gggtgcacga gtagtcaccg gacgcgttca
     4261 cgatgatgtc gatgttgggg gcgctcgcgc tcgtaccagg gctggtgagc gtcgagggtg
     4321 tattggtgaa cgacacatcg gtgggcggct ggacggcgta gacgaccgtc gtcggagtgt
     4381 cgccggtcgt cacataggtg gcctggaacg gcttggcttc gcccgactgg atcttggtcg
     4441 tgaacgagtc gatggtggcc ttgttgcctt tgatgtcgtg ggcgaccgtc ttgaccttgt
     4501 tgatagccgc gcacccgctc agcccgaacg ccacggcgac ggcggcggct actgctgcgg
     4561 ctcggtaagg gcgtgcgttc atggtcggcc tcccgtgcca gcgcccagaa tatgaagtct
     4621 catcgcggag gatagccaaa gccgcgatct gataccgaag accgatgaat ttcggtcgcg
     4681 tcgaccgtct gcctccagat ggccacttct gcacctggcc cacctgcggg cgtcccaggg
     4741 cgcacccccg cgcactcgcc gcgccccgtt gtcgagtgcg acgtggaggg cgcaaaccct
     4801 gatgcagtaa cggtcgaggc cctcgcgcga ctccaactca ttgcacggcg caacgggtgc
     4861 acgcttcgac tgcgcaacgc ctccgccgag ctgctcgacg tcatcgagtt gatgggactt
     4921 ggcagcgtgc tgacccagtc gtgaccccgc cttggccccg ccttggccct gac
//
LOCUS       DASWVF010000167         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_183067, whole genome shotgun sequence.
ACCESSION   DASWVF010000167 DASWVF010000000
VERSION     DASWVF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183067"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGD55_02865"
     CDS             complement(<1..267)
                     /locus_tag="VGD55_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869316.1"
                     /translation="MSVSIADGSVGTQVVLTVTDCPPRGSKPDQITWQGAAPPGEMAA
                     TRALSGIQRNGDELHVTYTVLPADAVGQGIFDAVCGNHGDHAGAT"
     gene            complement(524..1162)
                     /locus_tag="VGD55_02870"
     CDS             complement(524..1162)
                     /locus_tag="VGD55_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0869317.1"
                     /translation="MTTPSLLLPPLPDSPAAHAALDLIRANESTAIADHSVRSFLFAV
                     LLAEHRDVTDFDPSLLFFSCVLHDIGLSELGNRTQRFEVDGADVAADFLAEQGLSADD
                     RSTVWEAIALHTSAGIAERRGPVTELTRRGVGLDFGRDNECVSDQQAAAIHAAYPRLG
                     MERSLVDEIVAQAEERPQKAPRYSIAAELLRERSTVGTTVLEQGATLSRWGT"
     gene            1233..>1996
                     /locus_tag="VGD55_02875"
     CDS             1233..>1996
                     /locus_tag="VGD55_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0869318.1"
                     /translation="MRSHRVAILVLDGVLPLDFGIPSQVFLGRPWLPYRTTLCGERPQ
                     VEVATGYSLVVPGTLADVRRADTVIVPGFVDHERVFSNDVLDALRYVHRRGRRVVSIC
                     TGAFALAAAGVLDGLSATTHWRDADELAERYPQVTVDPNVLYVDNGQVLTSAGIASGI
                     DLCLHIVRRDLGAAAANQLARLIVAAPHRDGGQTQFIETPVGTSDGSLSATRSWALSH
                     LKEPLSVRDFARHAGVSERTVARRFVDETGSPPLQWL"
BASE COUNT          345 a          647 c          654 g          350 t
ORIGIN      
        1 ggtcgcccca gcgtggtcgc catgattgcc gcacacagcg tcgaagattc cctggcccac
       61 agcgtcggcc gggagaacgg tgtacgtgac atggagctcg tcgccgttgc gttggatgcc
      121 ggagagcgct cgagtcgcag ccatctcacc tggtggggcg gcgccctgcc acgtgatctg
      181 gtcgggcttg ctaccgcgcg gcggacaatc ggtcaccgtc aacaccacct gcgttccaac
      241 cgatccgtct gcaatggaaa ccgacatcag cgcggtggtt tgggtaacag aggtaagagc
      301 gttgggtgac gcggggacgg gctcggcgct ggccgatcca ctggaatgcg cactgcaaga
      361 cgcgatcagc atcgccaggc aaacaagaag aatgaatccg aacattgact cggtacggat
      421 gcgtgctgtt ccccgcgtcg gacacacgcg aagaagagta gcggtcgcag cgagacctga
      481 cccggtcggt tgacccttct caaggcagcg aaccgttggc ctgtcaggta ccccacctgg
      541 aaagagttgc accttgctcg aggacggtcg tgccgacggt cgagcgctcg cgcagcagtt
      601 cggccgcgat tgagtagcgc ggcgccttct gtgggcgttc ctcggcttgc gcgacgatct
      661 cgtcgaccaa cgaccgttcc atgccgagcc gggggtaggc agcgtgtatc gcagcggcct
      721 gctggtcact gacgcattcg ttgtcgcggc cgaagtcgag gccgacaccg cgacgggtga
      781 gttcggtcac cggcccgcga cgctcggcaa tgccggcaga cgtgtgcaac gcgatcgcct
      841 cccatacggt gctgcggtca tccgctgaaa ggccctgctc cgcaaggaaa tcagcggcga
      901 cgtcagcccc gtcgacctcg aaccgctggg tgcggttgcc caactcactg agtccgatgt
      961 cgtgcagaac gcacgagaag aacagcagcg acgggtcgaa gtcggtgacg tcgcgatgtt
     1021 cagcgagcag caccgcgaag agaaagctcc ggacgctgtg gtcggcgatc gcagtgctct
     1081 cgttggcgcg gatcaggtcg agcgcggcgt gcgccgccgg cgagtcgggc aagggcggta
     1141 acagcagcga cggtgtggtc atggtctgat cctggacccc tcgacctcag accacgagtg
     1201 gcaggattgc cactattcgc tagattaccg ccatgcgctc tcatcgagtc gcgatcctcg
     1261 tcctcgacgg ggtgttgcca cttgacttcg ggatcccgtc ccaggtgttc ctcggtcggc
     1321 cgtggcttcc ctaccggacg acgttgtgcg gcgaacgccc ccaggtcgag gtcgcgaccg
     1381 gctactccct cgtcgtacca ggcactctcg ccgatgtgcg ccgggccgac accgtcatcg
     1441 tgcccggctt cgtcgaccac gagcgcgtct tttcgaacga cgtgctcgac gcgctgcggt
     1501 acgtgcatcg gcgtggacgc cgcgttgtgt cgatctgcac cggagctttc gcgctagcgg
     1561 ctgccggagt actcgacggt ttgtcggcca ccacccattg gcgcgacgcc gacgagcttg
     1621 cggagcgcta cccacaggtc actgtcgatc ccaacgtgct gtacgtcgac aacggccagg
     1681 tactgacgtc ggcgggcatc gccagtggta tcgacctgtg cctgcacatc gtgagacgcg
     1741 acctcggcgc cgcagcggca aatcaactcg cgcggctcat cgtcgctgcc ccgcatcggg
     1801 acggcggcca gacgcagttc atcgagacgc cggtgggaac ctcggacggc tcgctatcag
     1861 cgacccggtc atgggcactc agccatctca aagaaccctt gtcggtaagg gatttcgccc
     1921 ggcacgcggg tgtttccgag cgcactgtcg cacgacgttt cgtcgacgag acaggatcgc
     1981 ccccgctcca gtggct
//
LOCUS       DASWVF010000168         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_284889, whole genome shotgun sequence.
ACCESSION   DASWVF010000168 DASWVF010000000
VERSION     DASWVF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284889"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /gene="dnaE"
                     /locus_tag="VGD55_02880"
     CDS             complement(<1..1913)
                     /gene="dnaE"
                     /locus_tag="VGD55_02880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0869319.1"
                     /translation="MAADSFVHLHVHTEYSMLDGAARLDDLFTETARMGMPALAMTDH
                     GNVFGAYDFYKKAKAAGVNPIIGMEGYLAPGHRSERKRVALTGGVGDDNAGEMYTHMT
                     LLAEDTVGMHNLFRLSSLASLEGYYYKPRMDRELLQTYAKGIIATTGCPSGEVNRLLQ
                     QGQYLAAKQAASDYRDIFGAENFYCELMDHGIEIERRHRDDLLKLARELKLPFVATND
                     LHYTYAKDWEAHEVLLCVQTGKTLADPNRFKFGEGQLYLKTPAEMREVWRDFPEACDN
                     TLAIAERVHVEFNEGANLMPQFPVPAGESEETWLVKEVEIGLRKRFPGGVPEGHRTQA
                     DYELGVICQMGFPGYFLVVADLVRYAQASSIRVGPGRGSAAGSMVSYALGITQLDPIH
                     HKLLFERFLNPERISMPDIDMDFDERRRGDMIRYATEKYGEERVAQIITYGTIKAKQA
                     VKDSARVLGFPFSTSDQITKVLPPALMGKDVSLAGIFDADNPRYGEAAEFRALYEGSP
                     DVKKVVDTARDLEGLKRQWGVHAAGVILSREPLLDVIPIQRRDQDGAIITQFDMGACE
                     ALGLLKMDFLGLRNLTVLDDCLAGIKENRGDEVVLETLDLDADKATYELLGRGDTLGI
                     FQLEGGGMRSLLRL"
     gene            complement(2107..2508)
                     /locus_tag="VGD55_02885"
     CDS             complement(2107..2508)
                     /locus_tag="VGD55_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY0869320.1"
                     /translation="MHTREVWVASGTQLVTDLTATIADFCRDLGDGLLNVFVPHATAG
                     VAVIEIGSGSEHDLQATIDRLWPTDAAYSHRHGSLGHGRDHVLPAFVAPSLVLPVVGG
                     TPTLGTWQSVILVDSNADNPERRVRLSFIPG"
     gene            complement(2495..3472)
                     /locus_tag="VGD55_02890"
     CDS             complement(2495..3472)
                     /locus_tag="VGD55_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0869321.1"
                     /translation="MSPATSDQGVLRTLEVPEGLDGERLDAALARLFGFSRTRAVELI
                     GQGRVQLDGAVPVKSDRVAAGSWLQVELPVVAAVGPAIVSTPVADMAIVYDDDDIVVV
                     DKPVGVAAHPSPGWDGPTVVGALVAAGYRVSTSGAEERQGVVHRLDVGTSGLMAVAKS
                     EAAYTALKQAFRDRTVDKTYHALVQGHPDPARGTIDAPIDRHPVHDYKFAVVADGKPS
                     ITHYETLEAHAMASLLEIKLETGRTHQIRVHMAALRHPCVGDLAYGADPTLAAKLGLT
                     RQWLHAARLAFAHPTTGRWIEFSSEYPADLARSLEIVRAGVEGERRAHS"
     gene            complement(3469..>4174)
                     /gene="lspA"
                     /locus_tag="VGD55_02895"
     CDS             complement(3469..>4174)
                     /gene="lspA"
                     /locus_tag="VGD55_02895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY0869322.1"
                     /translation="AVCVMQTTRGTSLDDEGEARGTSVEPSIEPGAAAAPGRHRAQGV
                     AAVDTGTPRRRHLGVLFAVAAFVLVADVVSKVIVVAKLSGRAPVRLLHGFLTLDYTRN
                     AGAAFSIGTGATYLFGIVAIAVILVILRTARRLFSTPWAIVLGLLLGGATGNLIDRLV
                     RSPGVLRGHVVDWIQLPHFAVFNLADSAISVGGVLAVLLALAGRQLDGTIARSSRAGH
                     RDAAPVGTVLPDESDS"
BASE COUNT          724 a         1378 c         1352 g          720 t
ORIGIN      
        1 agtcgcagca gcgaacgcat gccgccgccc tcgagctgaa agatgccgag ggtgtcgccg
       61 cgacccagca gctcgtaggt tgccttgtcg gcgtcgagat cgagggtctc gagaacgacc
      121 tcgtcaccgc ggttctcttt gatgccggcg agacagtcgt cgagcacggt gaggttgcgc
      181 aggccgagga agtccatctt gagaagtccg agcgcctcac atgcgcccat gtcgaattgg
      241 gtgatgatcg cgccgtcctg atcgcgccgc tggatcggaa tgacatcgag caacggttcg
      301 cgcgacaaga tgacgccggc agcatgcaca ccccactgcc gcttgaggcc ttcgagatcg
      361 cgcgcggtgt cgacgacctt cttgacgtca ggacttcctt cgtacaacgc gcggaactcg
      421 gcggcttcgc cgtagcgcgg gttgtcggcg tcgaagatgc ccgcgagtga gacatccttg
      481 cccatgagcg ctggcggcag caccttggtg atctggtcgg acgtcgagaa cgggaagccg
      541 agcactcttg ctgagtcttt gaccgcctgc ttggccttga tcgtgccgta tgtgatgatc
      601 tgcgcgaccc gttcctcgcc gtacttctcg gtggcgtagc ggatcatgtc accgcggcgg
      661 cgctcgtcga agtccatgtc gatgtcgggc atcgagatgc gttcggggtt gaggaaccgc
      721 tcgaacaaga gcttgtggtg gatcgggtcg agctgggtga tgcccaacgc gtaggagacc
      781 atcgagccgg ccgcactgcc acgaccaggc ccgacccgaa tcgagctcgc ctgcgcgtac
      841 ctgaccaggt cggcgacgac gaggaaatag ccagggaaac ccatctgaca gatgacaccg
      901 agttcgtagt cggcctgcgt gcggtgccct tctggcacgc cgcccgggaa gcgcttgcgt
      961 aggccaatct cgacttcctt gacgagccag gtctcttcgc tctcaccggc gggcaccggg
     1021 aactggggca tgaggttcgc gccctcgttg aactcgacgt gcacccgctc ggcgatcgcc
     1081 aaggtgttgt cgcaggcctc ggggaagtcg cgccagacct cgcgcatttc ggcaggtgtc
     1141 ttcaggtaca gctggccctc gccgaactta aagcggttcg gatcagccaa cgtcttaccg
     1201 gtctgcacgc agagcaagac ctcgtgtgct tcccagtctt tggcgtacgt gtagtgcaag
     1261 tcgttggtgg ccacgaacgg caacttcagt tctctggcca acttcagcag atcgtcgcga
     1321 tggcgtcgtt cgatctcgat gccgtggtcc atcagctcgc agtagaaatt ctcagcccca
     1381 aagatgtcgc gatagtcgga cgccgcttgc ttggcggcga ggtactgccc ctgctgcagg
     1441 agtcggttca cctcaccgga cgggcaaccc gtcgtcgcga tgatgccctt cgcgtaggtc
     1501 tgcaagagct cgcgatccat gcgcggcttg tagtagtaac cctcgagact cgccaggctg
     1561 ctgagtcgaa acaagttgtg catgccaacg gtgtcttcgg cgagaagcgt catgtgggtg
     1621 tacatctcac cggcgttgtc gtcaccgaca ccgccggtca gtgcgacccg cttgcgctct
     1681 gaccgatggc cgggcgcgag atagccctcc atgccgatga tcgggttgac ccccgcggcc
     1741 ttcgccttct tgtagaagtc gtacgcaccg aagacattgc cgtgatctgt catggcgagc
     1801 gcgggcatgc ccatgcgcgc cgtctcggtg aacaggtcgt cgagtcgcgc agcgccatcg
     1861 agcatcgagt actcggtgtg cacatgcagg tggacgaagg agtcagctgc catatctgcg
     1921 cgacctcccg tcgtccgcgc cctggttcgg cgcttgtgtt gcccgtgttg ctcgggacgt
     1981 cgaccggggg aagtcggcct tgccgttgcg agtcgtcgag ccctggggga cccgaccgca
     2041 tgactctaaa cccacccacc gacggccctt cggcgcgaca cgatggccaa cagtcccggc
     2101 ccgaaatcag cccgggatga agctcagccg gacccgacgc tcggggttgt cggcgttgct
     2161 gtcgaccaaa atgacactct gccaagtgcc gagagtgggc gtgccgccca cgactgggag
     2221 cacgagtgag ggcgcgacga acgcgggcag cacatgatca cgaccgtgcc cgagcgagcc
     2281 gtgccgatgc gaataggcgg cgtcggtcgg ccacagccgg tcgatggtgg cctgcaggtc
     2341 gtgctcgctc cccgacccga tctcgatcac cgcgaccccg gcggtcgcgt ggggcacgaa
     2401 cacgttcaga agaccgtcac caagatcgcg acagaagtcg gcaatcgtcg ccgtcagatc
     2461 ggtgacgagt tgggtgccgc tggcaaccca aacctcacga gtgtgcacgc cgctcgccct
     2521 cgacgcccgc ccgcacgatc tcgagcgaac gcgcgaggtc ggccgggtac tcactggaga
     2581 actcgatcca gcggcccgta gtcgggtgcg caaaagcgag acgcgctgca tggagccact
     2641 gccgggtcaa cccgagcttg gcggccaacg tcgggtctgc cccgtacgca aggtcaccca
     2701 cgcacgggtg ccgcaacgcc gccatgtgca cccggatctg atgcgtgcga ccggtctcga
     2761 gtttgatctc gagcagactc gccattgcgt gcgcttcaag agtttcgtag tgcgtgatgc
     2821 tcggcttgcc gtcggcaacc acggcgaact tgtagtcgtg cacgggatgg cgatcgatcg
     2881 gcgcatcgat cgtgccgcga gcaggatcgg gatgtccttg caccagcgcg tggtacgtct
     2941 tgtcgacggt ccggtcgcgg aatgcctgct tcagcgcggt gtacgccgcc tcgcttttcg
     3001 cgaccgccat cagaccgctg gtgccgacat cgaggcgatg caccacccct tggcgctcct
     3061 cggcacccga tgtcgaaacg cggtaccccg ccgcgacgag tgcgccgaca acagtcgggc
     3121 cgtcccatcc ggggctcggg tgcgcagcga caccgaccgg cttgtcgacc accacgatgt
     3181 cgtcgtcgtc gtacacgatg gccatgtcgg cgaccggcgt cgacacgatc gccggaccaa
     3241 ccgccgcaac aacgggaagt tcgacctgca gccacgaacc ggcggcgaca cggtcggatt
     3301 tcaccggcac cgcgccgtcc aactgcactc tgccttgtcc gatcagctcg accgcgcgcg
     3361 tccgcgaaaa gccgaacaac cgcgcgagcg ccgcatcgag tcgctcgccg tcgagtccct
     3421 cgggcacttc caaagtgcgc agaacacctt gatcggacgt cgcggggctc acgagtcgga
     3481 ctcgtcgggg agcactgtgc cgaccggtgc tgcatctcgg tggccggcac gacttgaccg
     3541 cgcgatcgtt ccgtcgagct ggcggccggc cagcgcgagt agcacggcga gcacaccacc
     3601 gacgctgatg gccgagtcgg ccaggttgaa cacggcgaag tgcggaagct ggatccagtc
     3661 gacgacgtgt ccgcgcagga cgcccggcga gcgcacgagc cggtcgatca ggttgcccgt
     3721 tgcgcccccc agcagcagcc cgagcacgat cgcccagggt gtgctgaaga gccgcctcgc
     3781 cgtgcgcaag atcaccaaga tcacggcgat ggcaacgatc ccgaacaggt aggtcgcgcc
     3841 ggtgccgatg ctgaaggccg cgcccgcgtt gcgcgtgtag tcgagcgtca ggaacccgtg
     3901 cagaaggcgc acgggggccc ggccagacag cttcgcgacc acgatgactt tcgagaccac
     3961 atcggcgacg agcacgaaag cggcgactgc aaacaggacg ccgagatgcc tccgccgtgg
     4021 ggttcctgtg tctaccgcgg cgacgccctg agcccgatgc cgccctggtg ccgccgccgc
     4081 gcccggctcg atgctcggct cgacgctcgt ccctcgcgct tcgccctcgt cgtctagcga
     4141 cgttcctcgc gttgtttgca tgacacacac agcg
//
LOCUS       DASWVF010000169         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_386959, whole genome shotgun sequence.
ACCESSION   DASWVF010000169 DASWVF010000000
VERSION     DASWVF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386959"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VGD55_02900"
     CDS             complement(<1..614)
                     /locus_tag="VGD55_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0869323.1"
                     /translation="MRLIGRLAAPEPGWTASADIVVVGSGIAGLTTALKARQAGRVLL
                     VTKTELDAGSTRWAQGGIAAALGPGDSPADHLTDTLVAGAGLCDEEAVRVLVTEGPDL
                     VRELVDLGTRFDLDNAGDLALTREGGHLRRRIAHAGGDATGAEIERALIAAVKATEGI
                     EIIEHALVIDLLKTADGRACGVTLHVIGAGRRDGVGAALGRAVVL"
     gene            complement(611..1450)
                     /gene="panC"
                     /locus_tag="VGD55_02905"
     CDS             complement(611..1450)
                     /gene="panC"
                     /locus_tag="VGD55_02905"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0869324.1"
                     /translation="MTDVAHTRAELAAARARLQGTVALVPTMGALHEGHRSLLRHARD
                     AADTVVVSIFVNPLQFAPGEDLDRYPRPLEADLAMCTDEGVTLAFVPSRLEMYPREPV
                     VRVCAGPMGERFEGASRPGHFDGVLTVVAKLFGLVRPDVALFGRKDAQQLALIRRMVA
                     DLELPVRIEASPLIRDADGLALSSRNKYLSADERLAALALSRAVAAGAAAHGRVAAIV
                     AAARQVLDAQGGVESDYVAVVDPDTFDEIGASSTSGLLVLAARVGSTRLIDNGCVDLG
                     ALS"
     gene            complement(1447..2358)
                     /locus_tag="VGD55_02910"
     CDS             complement(1447..2358)
                     /locus_tag="VGD55_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY0869325.1"
                     /translation="MTAAHPLKAHARPGRLAVGVISAGRVGAVLGAALRQAGHRIIAV
                     SAVSSDSLRRASALLPGVEVRTPDAIGPDVDLLLLAVPDDALPALVEGLANAGALRPG
                     QFVAHTSGRYGIDVLAPASAIGALPLALHPAMTFTGTSVDLDRLTGVCFGVTSRVALR
                     PVAEALVLEMGGEPVWIDEVDRPLYHAALAHGANHLVTLVASSMDLLRAAGVDDPQRL
                     LGPLLSAALDNALRSGDAGLTGPVVRGDAGTLAAHLDVLGRVSPETTAAYIAMARLTA
                     DRALAAGLLPADDAAELLGVLSRGSNE"
     gene            complement(2399..>3136)
                     /locus_tag="VGD55_02915"
     CDS             complement(2399..>3136)
                     /locus_tag="VGD55_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0869326.1"
                     /translation="QPGVFVGALPGVISVFQTVWQTIQQHFGFTVADSPDGLRLRHGL
                     IETRAQTVPPGRVQAVRIVQPLLWKLFGWVRVDANIAGYAGHRAEDGRQHNSVLLPVG
                     SRAQALLVISRVLPGVDIEAVPMTRVPSRAKWRSPFLWWTYRAGADEAVVSVRSGLLT
                     KVHAVMRHEKIQSVRLSAGLWQRALRLATVHIDSTKGPVGVVLHNRDIAEGRRIVDIE
                     VVRAEHGRALRAPDRWMTTGSGTMAGR"
BASE COUNT          525 a         1098 c         1068 g          445 t
ORIGIN      
        1 agcacgaccg cgcggcccag cgcggctccg acgccgtcgc ggcgaccggc tccgatcacg
       61 tgcagcgtga cgccacacgc gcgaccgtcg gcggtcttca gcaggtcgat caccagcgcg
      121 tgctcgatga tctcgatgcc ctctgtggct ttcacggcag cgatcagcgc tcgctcgatc
      181 tcagctccgg tcgcgtcgcc accggcgtgc gcgatccggc ggcgcaggtg cccgccttcg
      241 cgagtcagcg cgaggtcgcc cgcgttgtcg aggtcgaaac gggtgccgag gtcgacgagt
      301 tcgcgcacga ggtcagggcc ctcggtgacc agtactcgca cggcttcttc gtcgcacagc
      361 cccgcaccag cgaccaacgt gtcggtcagg tggtcagctg ggctgtcgcc tggcccgagc
      421 gccgctgcga tgccaccctg cgcccatcgc gtcgagccgg cgtcgagctc ggtcttggtg
      481 accagcaaca cccggccggc ctgccgagcc ttcaaggcag tcgtcagtcc ggcgatgccg
      541 ctgccgacca cgacgatgtc ggcggaagcg gtccagccag gttcgggcgc ggcaagccgg
      601 ccgatcagcc tcatgacaac gctccgagat ccacgcagcc attgtcgatg agccgcgtcg
      661 agcccactcg ggcggccagc acgagcagcc ctgatgtgct cgacgcgccg atctcgtcga
      721 acgtgtccgg gtcgacgacg gcgacgtaat cgctctcgac gccaccctgt gcatcgagca
      781 cctggcgggc cgccgccacg atggcggcaa cccgtccatg agccgcggcc cctgcggcca
      841 ccgctctcga cagcgcgagc gcggcgagtc tttcatcagc ggataaatac ttgttgcgac
      901 tcgacaacgc cagcccatcg gcgtcccgta tgagtggcga agcctcgatg cgaacgggca
      961 gctcgaggtc ggcgaccatg cggcggatga gggcgagctg ctgcgcatct ttgcggccga
     1021 agagggcgac gtcggggcgc acgagaccga acagtttcgc gaccacggtg agcacgccgt
     1081 cgaagtgacc tggcctgctc gccccctcga agcgctcacc catcgggcct gcgcacacgc
     1141 gcacgaccgg ctcgcgcgga tacatctcga gccggctcgg gacgaacgca agtgtgacgc
     1201 cctcgtcggt gcacatcgcg aggtcggctt cgagcggtcg cggataccgg tcgaggtctt
     1261 cgcccggtgc aaactgcagc gggttcacga agatgctgac gacgacggtg tcggcggcgt
     1321 cgcgtgcgtg ccgcaacaag gagcggtgac cttcgtgcaa cgcgcccatc gtcggcacga
     1381 gcgcgacggt gccctgcaac cgtgcgcgtg ccgccgcaag ttcagctcgg gtgtgtgcga
     1441 catcggtcat tcgttcgaac ctcgagacag gacgccgagg agttcggccg catcgtcggc
     1501 cggcaacaaa cccgcagcga gtgcgcgatc cgcagtcaac ctggccatcg cgatgtacgc
     1561 cgcggtggtc tcgggcgaca cgcggccgag cacgtcgagg tgtgcggcaa gggtgcccgc
     1621 gtcgccccgc acaacagggc cggtcaagcc cgcatcgcca gagcgcagcg cgttgtcgag
     1681 ggccgccgaa agcaacggcc cgagcagccg ctgcggatcg tcaacgccgg ccgcacgcaa
     1741 cagatccatc gacgacgcga cgagagtgac gagatggttg gcgccgtgcg caagggccgc
     1801 gtgatagagc ggccggtcga cctcgtcgat ccagaccggc tcgccgccca tttcgagcac
     1861 caatgcctct gcgaccgggc gcagggcgac ccgcgacgtg acgccgaaac acacgcccgt
     1921 cagtcggtcg aggtcgacgc tggtgccggt gaacgtcatg gcagggtgca atgccagggg
     1981 tagcgcacct attgcagaag caggggccag cacatcgatg ccgtaacggc cggacgtgtg
     2041 cgcaacgaac tgtcctggcc gcagggcgcc ggcgttggcg aggccttcga cgagcgcggg
     2101 gagtgcgtcg tcgggcaccg cgagcagcaa caaatcgacg tccggcccga tggcgtcggg
     2161 tgtacgcacc tcgactccgg gcaacaaggc cgatgcgcga cgaagggagt ccgacgacac
     2221 cgccgagacg gcgatgatgc ggtgacctgc ctggcgcagc gcggccccaa ggaccgcgcc
     2281 gacgcggccg gccgagatga ccccgaccgc aagccgccca ggtctcgcat gggccttcag
     2341 cgggtgcgca gcggtcacgt tggttccagt cctcccgggg taccggacgc tgccgagcct
     2401 acctgcccgc catggtgccc gagccggtcg tcatccagcg gtcgggcgca cgcaaggccc
     2461 ggccatgctc ggcccgcacg acctcgatgt cgacgatgcg gcggccttcg gcgatgtcgc
     2521 ggttgtgcag caccacgccg accgggcctt tggtcgagtc gatgtgcacg gttgcgagcc
     2581 gcagagcgcg ctgccacagc ccggccgaca ggcgcacgct ctggatcttc tcgtgccgca
     2641 tgactgcgtg cacctttgtc agcaatccac tgcgcacgct gacgactgcc tcgtcggcgc
     2701 ccgcgcgata ggtccaccac aggaatggcg atcgccactt cgcccgcgac ggaaccctcg
     2761 tcatgggcac cgcctcgatg tcgacaccgg gcagcacccg gctgatgacg agcaacgcct
     2821 gcgcccgcga accaacgggc aacagcaccg agttgtgctg gcggccatct tcggcgcggt
     2881 gccctgcgta gccggcaata ttggcgtcga cgcgtaccca gccaaacagc ttccacagca
     2941 acggctgcac gatgcgcacg gcctgcaccc gacctggcgg caccgtttgg gcacgcgtct
     3001 cgatgagtcc gtggcggagg cgaagtccgt caggtgaatc agcgacggtg aagccgaagt
     3061 gctgctggat cgtctgccac accgtctgga agacgctgat gactccgggc agtgcgccga
     3121 cgaagactcc gggctg
//
LOCUS       DASWVF010000170         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_234105, whole genome shotgun sequence.
ACCESSION   DASWVF010000170 DASWVF010000000
VERSION     DASWVF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234105"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGD55_02920"
     CDS             complement(<1..344)
                     /locus_tag="VGD55_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869327.1"
                     /translation="MVAPHPDDEFALWSMAQDRGHFPVLLVLTHGEKAAACGGAGLQS
                     AAGERIPSPPPSTPPSTAICSAERIDSLLAFLTDLGSSDPRWSGLTELPPRTGVRVWR
                     AAGAALLVFDLG"
     gene            379..1395
                     /locus_tag="VGD55_02925"
     CDS             379..1395
                     /locus_tag="VGD55_02925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869328.1"
                     /translation="MPAAVAVGLAGDMGPEPVGVDRLAPDGLAVAFGFVPEPLHEAVA
                     TTATQIRVAQKAVPTPRRPPIAFDSRTTTLRKAPYDARVTNTRMRHVQAVQADYDTRS
                     PRWAHVYEGASYHDAVLQERLAIALGMLGRRDRATSGLALDVGCGAGQLLEALHAEGT
                     PVVGLDVSWQQVTLSRERVPAAVFVAQADGEQLPLADGTVATLTALGLLEYLPSLHDG
                     LEEFARVTMPGAYVVVSMPNPLRLAYLCDPIGVVLGRLRKTRPGYRRSYRSARAFAAE
                     LQACGFDVIELRGHGLGRFTLAGRPILGDAKSVRLSALLQARLPDAASRLFGANLVAI
                     ARRA"
     gene            1392..2204
                     /locus_tag="VGD55_02930"
     CDS             1392..2204
                     /locus_tag="VGD55_02930"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY0869329.1"
                     /translation="MMRHVLTSAVSRRLGRGAQRLSPSARSAINALRGIDQPRLVVLP
                     PGSRVLCVAPHPDDELLGCGGTLAKHRAAGHDVHVVFVTSGEGSAGAHGTRQSAVEQR
                     ETEAARAMQVALPEATSQFLRLRDGCLTDQRVALELALDDVIAASGADLVYAPHPGER
                     HGDHVAVSEALIRVLSARDAGPHVEHVAWYEVWSPLPATCIVDVTAEFSCKIKGLECY
                     ESQLQITGYARTAQGLAAYRSAQPLKGHGYAEAFVVMSRVELTRWAEQAPLT"
BASE COUNT          372 a          732 c          840 g          416 t
ORIGIN      
        1 tcgccgaggt cgaacacgag cagcgccgcg ccggccgctc tccacacgcg tacgccagta
       61 cgcggtggca gttcggtcag ccctgaccac cgagggtcag atgagcccag gtcagtcaga
      121 aatgccagga gcgaatcgat ccgttcggcg ctgcagatcg cggtcgatgg cggggtggac
      181 ggcggcggtg acgggatgcg ttcgccggct gccgactgga gacccgcacc accgcatgcg
      241 gccgccttct cgccgtgggt gagaaccagc aacaccggga aatgaccgcg atcctgcgcc
      301 atcgaccaca gcgcgaactc gtcgtcgggg tgcggtgcga ccaagtagcg gatggggagc
      361 gcaggcgatg cggaagtcgt gccggccgct gtggcggtcg ggctcgctgg cgacatgggt
      421 cccgagcctg tcggcgtcga ccggctcgcg cccgacgggc tcgcggtcgc tttcgggttc
      481 gtgcctgagc cactgcatga ggccgtcgcg acgacggcaa cacagatcag ggtcgcccaa
      541 aaggcggtgc ccacacctcg tcgtccgccc atcgccttcg attcgcgcac gacgaccctt
      601 cggaaagctc cctacgatgc acgggtgacc aacacgcgga tgcggcatgt gcaggccgtg
      661 caagcagatt acgacacgcg atcgccgcgt tgggcacacg tctacgaagg cgcgagctat
      721 cacgacgcgg tgctgcaaga gagattggcg atcgcgcttg ggatgttggg ccgtcgagac
      781 cgcgccacct ccgggctcgc actcgatgtg ggctgtggtg ccggtcagtt gctcgaggca
      841 ttgcacgctg aaggcacgcc agttgtcggg ctcgacgtgt cgtggcagca ggtgacgtta
      901 tcgcgcgaac gggtgccggc tgcggtgttc gtcgcgcagg ctgacggcga gcagttgccg
      961 ttggccgacg ggaccgtcgc gacgctgacc gcgttaggac tgctcgaata cctgccgtca
     1021 ttgcacgatg gactcgagga attcgcccgc gtcacgatgc cgggtgcata tgtcgtcgtc
     1081 agcatgccga atccgctgcg gctcgcctat ctgtgcgacc cgatcggtgt cgtgctcggc
     1141 cggctgcgaa agacgcgacc ggggtatcgg cgcagctatc gttctgcgcg tgcatttgca
     1201 gccgagttac aggcgtgcgg attcgacgtg atcgagctgc gcgggcacgg actcggccga
     1261 tttaccttgg ccggccgacc cattctcggc gatgcgaaat cggtgcggct gtcggcgctg
     1321 ctccaggcga ggcttccgga cgctgcctcg cggttgttcg gtgcgaacct cgtcgcgatc
     1381 gcgcgtcgcg catgatgcgg catgtactca ccagcgccgt ctcgcggcga ctgggtcgcg
     1441 gcgcgcagcg gctttcgccg tcggcgcgaa gtgcgatcaa tgcgttacgc ggcatcgacc
     1501 aaccccggtt ggtcgtcttg ccgcccggca gcagggtgct gtgcgttgct cctcatccgg
     1561 acgacgagtt gctcggctgc ggcggaacgc tagcgaagca tcgggcggcc ggccacgacg
     1621 tccacgtggt cttcgtcacg agcggtgagg ggagtgcggg cgcgcacggg acgcgacaat
     1681 cggcggtcga gcaacgtgaa accgaggcag cacgggcaat gcaggttgcg ctgcccgaag
     1741 cgacgtcgca gttcttgcgg ttacgcgatg gttgcctgac cgatcagcga gtggcgctgg
     1801 agctcgctct cgacgacgtc atcgcggcgt cgggggccga cttggtgtac gcgccgcatc
     1861 ccggtgagag gcacggtgac cacgtcgcag tgtcggaggc gctcattcgt gtgttgtcgg
     1921 cgcgcgacgc tgggcctcat gtggagcacg ttgcttggta tgaggtctgg agcccgctcc
     1981 cggcgacctg catcgttgac gtgaccgccg aatttagctg caaaataaag ggtttggagt
     2041 gctacgaatc gcagctacag atcactggct acgcgcgaac cgcacaagga cttgccgcgt
     2101 accgatctgc gcaaccgttg aaaggtcacg ggtacgccga ggcgttcgtg gtgatgtcgc
     2161 gcgttgagtt gactcggtgg gctgagcagg ctccgctgac gtgagcatca cacccggcga
     2221 cgtgcttggc catggccgcg aagcgatcgt gcacgcgctc gagcctgatc gcgtgctgaa
     2281 ggtgtttcgt gacgccggcg ccgggtcacg tgtacgggcg gaattcgatg tcggcctgct
     2341 gttgcatgcc gccgggcccc
//
LOCUS       DASWVF010000171         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_30189, whole genome shotgun sequence.
ACCESSION   DASWVF010000171 DASWVF010000000
VERSION     DASWVF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30189"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGD55_02935"
     CDS             <1..927
                     /locus_tag="VGD55_02935"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0869330.1"
                     /translation="HPVHVPGVHNRVAVETLMLPSIRGIDRWLLPNYYTPIGLRGRVV
                     TVIHDAQHEHFPENFSTRKRAWLRAAHRYTMRRADTVVAISSFVADDLIRLHGSHYAD
                     KISVIPNAVSFDRLEPGSVPPGVPANREVVLCVSGSYRHKNLQTLVRAFAEVAARRPE
                     SHLVIVGQAPELLLGARQATPVSVLVGELDLADRVTSLGYVSDATLGALYRRASVVAM
                     PSLFEGFGLPVIEALGLGVPVVSTRCGALEEVSLGLAHYVDDPLSSPELASRLVDMLV
                     EGYKPSAADVEQVRARYDPRRVAGLYAAAATG"
     gene            complement(917..>1595)
                     /locus_tag="VGD55_02940"
     CDS             complement(917..>1595)
                     /locus_tag="VGD55_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869331.1"
                     /translation="ARRLQLVVVFVVVTQIAMLGIAHAIVGAGALRNSYLYVNPLLRL
                     YEFAIGIALALAVQRGWRPTISPAVAFLVLLVGVVGVSIPNHALPSYLGGLLALPGIV
                     LVIASCATRELIGAPVRFLMHRLLVRLGVWSFALYMTHQLLLRTMVAEYNRYRLESVP
                     QFIAYPAFLALAVAVAGIFSTFVEQPAERRLRRAVSRGPAVSTADELTPTSPPRDTRP
                     IGVAVTP"
BASE COUNT          294 a          526 c          495 g          280 t
ORIGIN      
        1 cacccagtgc atgtaccggg cgtgcacaac agggtggcgg tcgagaccct gatgctgcct
       61 tccattcgcg ggatcgaccg ctggctgctg cccaactact acacgccgat cgggttgcgt
      121 ggccgcgtcg tcaccgtgat acacgacgcc caacacgagc acttccctga gaacttctcc
      181 acgcgcaaac gggcatggtt gcgcgccgcg caccgttaca cgatgcgtcg agccgacact
      241 gtggtcgcta tctcatcgtt cgtcgccgac gacctcattc gcctgcacgg ttcgcactac
      301 gccgacaaga tcagcgtgat tcccaacgcg gtttcgttcg atcggttaga acctggttcg
      361 gttccgcccg gcgtcccggc caaccgcgag gtcgtgctct gcgtctcggg cagctaccga
      421 cacaagaacc tccagacgct cgtgcgtgcc ttcgccgagg ttgctgcccg ccggcccgag
      481 tcgcacctcg tgattgtcgg ccaggcacct gaactactgc tcggcgcgcg acaggcaacg
      541 ccggtgtcgg tgctggtggg cgagctcgat ctcgcggatc gcgtgacctc gctcggatac
      601 gtcagcgacg cgacgctcgg tgcgctgtac cggcgtgcgt cggtggtggc gatgccgtcg
      661 ctcttcgaag gattcggcct gccggtaatc gaggcgctcg gccttggcgt ccccgtcgtc
      721 agtactcgat gcggcgcgtt ggaggaggtc tcgctcgggc tcgcgcacta cgtcgatgac
      781 ccgctgagtt cgcccgaact ggcttcccgt ttggttgaca tgctcgtcga gggctacaaa
      841 ccttcagcag cggacgtcga acaggtgcgg gctcgttatg accctcgccg agtcgccggc
      901 ttgtatgcgg cagcggctac ggggtaactg ccaccccgat cggccgcgta tctcggggcg
      961 gcgatgtcgg cgtcaactca tctgctgtcg aaacagcggg cccgcgtgac accgctcggc
     1021 gcaaccggcg ctcggctggc tgttccacga aagtagagaa gatgccggca accgcgacgg
     1081 ccagggccag gaacgccggg tacgcaatga actgaggcac cgattccaat cgataccggt
     1141 tgtattccgc aaccattgtt cgcagtagta gctggtgcgt catgtacaga gcaaatgacc
     1201 acacaccgag ccgaaccagc agtcgatgca tgaggaatcg cacgggcgcc ccgatgagct
     1261 cgcgcgttgc acacgaggca atcaccaaaa cgatcccggg caacgcgagc aggccgccga
     1321 gatacgacgg cagtgcgtgg ttcggaatgc tcacaccgac aacgcctacc agcagtacca
     1381 ggaacgctac ggctggtgag attgtcggcc gccaaccgcg ttgtaccgcc agcgcaaggg
     1441 cgatgccgat cgcaaactcg tacagccgca gcagcgggtt cacatagagg tagctgttcc
     1501 gcaacgcacc agctcccacg atcgcatgcg cgatccccag catcgctatc tgggtcacga
     1561 ccacgaagac gacgacaagt tggagtctcc gggcg
//
LOCUS       DASWVF010000172         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_132262, whole genome shotgun sequence.
ACCESSION   DASWVF010000172 DASWVF010000000
VERSION     DASWVF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132262"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1077
                     /locus_tag="VGD55_02945"
     CDS             97..1077
                     /locus_tag="VGD55_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEY0869332.1"
                     /translation="MDLILQRPDLLDDAAWLAVEQHRVRLASALSSNDAPWIIGSAKE
                     LVECVARVVSTAKGTITPSNADFADVVNDAHVALDRQAGPGITRASDVRAIAQNAKKI
                     VLSVKDIRNDFGTGHGHAEVRSIADEMVQLVVAGALLWVRWALVRVAHLILNEPAHLI
                     AELRGPVTRRSLQSHLDALVLPEPPDDIQRALGIAFAHRSASGTFVAREVGLEAPAKS
                     DNTELWPGPYRLGVVEGLTISSNGYFMLGVDWVPMVVDILLPLPPTTIKNFLRDLTAK
                     VTSAGSLPNASVEDHTNLARGSEGEVDRLPEAARVAWLDFATEVDPTASE"
     gene            complement(1574..>1902)
                     /locus_tag="VGD55_02950"
     CDS             complement(1574..>1902)
                     /locus_tag="VGD55_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869333.1"
                     /translation="AAATGMIKSAAGAASYLVERANKCANLTGWYEGIIDGAGLRKND
                     PRLLFRRVMFAMARKQAGTVMRRRDTREHVALYLKAFNAWATNEDLPQLRFNSRDTMP
                     PIAKAR"
BASE COUNT          324 a          587 c          634 g          357 t
ORIGIN      
        1 ccgtcgagga ataccgtgcc gccggagtgc cgcagccgag gcacacgggc cgttcacgtc
       61 cgtgcactgg ccggcttcgg ggcggacaat ggctatgtgg acttgatcct tcagaggccg
      121 gatctcctcg acgacgccgc ctggcttgcc gtcgaacagc accgagtacg cctcgcgtca
      181 gccctgtcct ccaacgacgc gccgtggatc atcggcagcg ccaaagaact cgtcgaatgc
      241 gtcgcgcgcg tcgtatccac ggccaagggc acgataacgc cgagcaatgc ggatttcgcc
      301 gacgtggtca acgatgctca tgtcgccctc gaccgacagg caggcccggg tattacccgt
      361 gcttccgacg tccgggcgat cgctcagaac gctaagaaga ttgtgctcag cgtgaaggac
      421 atacggaacg acttcggtac cggtcacggc cacgccgagg tcaggagcat tgcggacgag
      481 atggtgcagc tcgtcgttgc aggtgcgttg ctgtgggtcc gctgggcgct ggtccgagtc
      541 gcacacctga tcctgaacga gcctgcgcat ctgattgctg agcttcgagg acccgtgact
      601 cgccggtcgc ttcaaagtca cctcgatgcc ttggtccttc cagaaccgcc ggacgatatc
      661 caacgtgcgc tcggcatcgc cttcgcccac cgttccgcca gcggtacgtt cgtcgcgcgg
      721 gaggtcgggc tggaggcccc tgccaagtcc gacaacacgg agctgtggcc ggggccctac
      781 cgcctcggcg tcgtcgaagg tctgacgatt tcaagcaacg gctatttcat gctcggcgtc
      841 gactgggtac cgatggtcgt cgacatcttg ctcccgctgc cgccgaccac catcaagaac
      901 ttcttacgtg acctgacggc gaaggtgacc agtgcgggct cattgcccaa cgcgtcggtt
      961 gaggaccaca ccaaccttgc ccgcgggagc gaaggcgagg tggaccggct gcctgaggcc
     1021 gcccgcgtcg cgtggctgga tttcgcgacc gaggttgacc cgaccgcgtc ggagtaggcg
     1081 tcgggcgagt gatcgcatcg cgcagtagcc cgaccccctg cgtggtgcgg tgcctggctg
     1141 acaccggcac cgccaaggcg caggcatcaa ctcccagttc gacacactga gcgaaggatg
     1201 ggggagattc gggtggcgac ctgtagggac atcaacatag ccaccggcgg ggtactaatt
     1261 gctccgaaac accgacttga acccgaaccg cagattaaga gacgcgtcag ggcggtatca
     1321 gccgggttca tccagttgtc ctgagatcgg gtttgcgcag ctcaaaaccg tgagtgagtt
     1381 cgcggggtat ccaccgatga tgcctcgtgc cgacacgtcc gtgatcacca cgtgaccacc
     1441 atcatgatca tttgggggtc gcgagacctg ggatggcgtg aattcgccga cggtccgaca
     1501 ccgctcgtcg gtgctggcga agcgtgctcc ggcggccgca tggtcgtgcc cggttgtcgg
     1561 ggcgtttggt cggttagcgg gctttggcga ttggtggcat ggtgtcgcgg gagttgaacc
     1621 gcagctgggg gaggtcttca ttggtggccc aggcgttgaa tgctttgagg tagagggcga
     1681 cgtgttcgcg ggtgtcacgt cggcgcatga cggtgccggc ttgtttgcgg gccatcgcga
     1741 acatgacccg gcgaaacagc agcctcgggt cgttcttccg caggccggcg ccatcgatga
     1801 tgccctcgta ccagccggtg aggttggcgc atttgttcgc gcgctcaact aggtagctgg
     1861 cggcgccggc cgcgcttttg atcattccgg tcgcggcagc ga
//
LOCUS       DASWVF010000173        12359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_208689, whole genome shotgun sequence.
ACCESSION   DASWVF010000173 DASWVF010000000
VERSION     DASWVF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208689"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VGD55_02955"
     CDS             <1..1074
                     /locus_tag="VGD55_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0869334.1"
                     /translation="DELARPVLLASWTRHAVSELTLHDLATGERIGEVPLPSLGSVGG
                     LSERPEGGPECWFGYTDHTTPASVFRYDARTGATTSWANAPGIVEVPKVHSRLVTYTS
                     RDGTPVRMIVVSTDAEPDHPRPTILGGYGGFGASMTPGYSATMLAWVEAGGIYAVANL
                     RGGGEEGESWHRAGMRGNKQNVFDDFHAAADWLVAAGYTTPSQLGISGGSNGGLLVGA
                     ALTQAPEKYAAVACSAPLLDMVRYEKFGLGQLWSEEYGSAEDPEELGWLIAYSPYHHV
                     VEGDAYPAVLFTVFDSDTRVDPLHARKMCAALQAATSASRPIVFRRESDVGHGARAVS
                     RSVDLSADTLAFLGAHTGLAFGD"
     gene            complement(1137..2429)
                     /locus_tag="VGD55_02960"
     CDS             complement(1137..2429)
                     /locus_tag="VGD55_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0869335.1"
                     /translation="MRNRLLILAAATTTLVIVAFLVPLSVLVRNLAHQRALNDGLKES
                     QRVVTAVQTGERTQAALAQAVTGSPTGISVSVRLGDGVWVGTQPADPDSPALHLAVGP
                     NGTELREVQVRYKGGLEIWHPVTDTAGKPIVVRTFVSAALFDKGVFSAIVILVLVGIA
                     ILAVAMVIADRLAQWTVRPVAALASTANRLSSGQLQARAPVIGPREVVEVGVAMNRLA
                     QRITELLDDEREQVADLSHRLRTPVAAVKLAVEALPDSPEAEKLSEHVDALERTVGSV
                     IREARRGVREQPAPACDVVSVVKGRVAFWAVLAEDQGRRVELRLPTTSRFVRVSADDL
                     AAAVDALLENVFAHTPEGTAMSVAVEVDRTSAWITVSDDGPGIGAGSIDRGRSAGGST
                     GLGLDIVRRTAEGSGGSFEVGSSPNGGAAMVIRLGPAD"
     gene            complement(2447..3139)
                     /locus_tag="VGD55_02965"
     CDS             complement(2447..3139)
                     /locus_tag="VGD55_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869336.1"
                     /translation="MPRLLLIEDDAAIRTSLTRSLSERGHAVASAPTAMAGLQAVVDT
                     APDLVVLDLGLPDLDGGTLIQMVRAVSKVPIIVATARDDQAEMVRILDWGADDYVVKP
                     FASDHLEARIRAVLRRMASGDAAQAVVVGGLRIDQRTREASLDGEKLELTRKEFDLLL
                     YLAEHEGEVVSKRVLLAEVWQQAYGGADKTVDVHLSWLRRKLGESAATPRYLLSVRGV
                     GVKLVGPTEPDS"
     gene            complement(3490..3915)
                     /locus_tag="VGD55_02970"
     CDS             complement(3490..3915)
                     /locus_tag="VGD55_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869337.1"
                     /translation="MGELDVEAPEAEGDGDVEMDDEGELVGVEDPLGVGVGVGVEEAV
                     TAPPTALPSTQVYTYSIPGGVGSEADGDGDTGGGVVFTAGVTETEAFGLECDGLGDVV
                     GSFVGATVGVMTGCARNGEAELADAADADCWATCALTNC"
     gene            3950..4090
                     /locus_tag="VGD55_02975"
     CDS             3950..4090
                     /locus_tag="VGD55_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869338.1"
                     /translation="MPGGRIVIACLGDTIANASATTTDPGYTLTPRSLVDPRVDVFFS
                     KN"
     gene            4344..5618
                     /locus_tag="VGD55_02980"
     CDS             4344..5618
                     /locus_tag="VGD55_02980"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEY0869339.1"
                     /translation="MKLAVVGGGSTYTPELVDGMARLRDQLPLEELVLVDPDPHRLEL
                     VGGISRRIMAAQDHPAKVSWTSDLDAGIDSADAVVLQLRVGGQAARHKDESWPLECGC
                     VGQETTGAGGLAKALRTIPVVLDIAERAEKHAAPGAWIVDFTNPVGIVTRALLDGGHK
                     AVGLCNVAIGLQRYFAQLLGIDPTTVHLDHVGLNHLTWERAVTVNGVDVLPQLLADHG
                     DEIASHIELPLAVLRQLGVVPSYYLRYYYEHDNVVDELRGQPTRAQVVAGVEQELLDI
                     YADPTVSTKPALLEQRGGAYYSEAAVNLISSLERDTGDVQVVNVRNAGTLPFLADDAV
                     IEVPAIVGADGAKPLPIAQVDPLYAGLIAHVTAYEELAVDAAIHGGRDRVFKALLAHP
                     LVGQIDLANGLTDRLLAANYEHLPWVSKSTSR"
     gene            5615..6676
                     /locus_tag="VGD55_02985"
     CDS             5615..6676
                     /locus_tag="VGD55_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0869340.1"
                     /translation="MNDGPPVAVLAIDGGNSKTDVALVSVEGDLLGHSRGRPALAPEI
                     GMEPALRIISGLVDEAAQDAGLTRDAGPLAQHTSAYLAGVDIPQEQVAMHQALLEQGW
                     SSTVRVDNDTFAIFRAGTTQHWGVGVVSGAGMNAVGIGRDGRVGRYLALGEFTGDFGG
                     GNDLSYQVMYWTFRDEDGRGGETALTPAVCEHFGLPTIYDVALAMHRGEITKTQRLEL
                     TRVLFEVAATTADPVAVSLLERQAHEVALMARQLLRDLDLIDVETDIVLGGGVLTSRD
                     PLLTRLIRQSMAAEAPKGRVTINEVPAVTGAALLGFDHLGLGRTAEDRLRSQMRSTAD
                     TSAVKNAVSTHRMLHRFTK"
     gene            complement(6666..8843)
                     /locus_tag="VGD55_02990"
     CDS             complement(6666..8843)
                     /locus_tag="VGD55_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEY0869341.1"
                     /translation="MSGNAVRLQAIKDVEAYVPPAVSYLASEAPGEIFGANVFSKTVM
                     QQRLPKSVFKSVMATIEHSAPLDPLVADAVAAAMKDWAMEKGATHYAHVFYPLTGLTA
                     EKHDSFLEPAGDGSALAEFAGKTLTQGEPDASSFPSGGLRNTFEARGYTGWDVTSPAY
                     VLENPNGNTLCIPTIFVSMTGDALDHKTPLLRSQQSMAVHAERILKLFGHAHPDHVVS
                     FCGAEQEYFLIDRHFFLARPDLVNAGRTLFGSKPPKGQEFDDHYFGAIPERVLGFMMD
                     TERELFKLGIPAKTRHNEVAPGQFEIAPMFERANVAADHQQLLMSTFKRLAQKHGMEC
                     LFHEKPFLGINGSGKHVNFSLGNSTEGNLLLPGDTPHENAQFLVFCAAVIRAVHKYGG
                     LLRASVASASNDHRLGANEAPPAIISIFLGDQLTDVFDQIAKGGATSSKEKGTLTIGV
                     DTLPNLPTDPGDRNRTSPFAFTGNRFEFRAPGSLQSVAGPMVTINTIMAEALDYIATS
                     LETALASGTEFNVAVQKILEEIITVHGASVFNGDGYSEQWQVEAAQRGLPNLRTTLDA
                     LPELISEPSMELFSKYGVFTHREMHSRYEIGLEQYALSVGVEARLTLEIGSTLVLPAA
                     VRYQTEVALNLGALKAAGVEVDPSTLDDVSAPLKALRAALGILRDELAPDSGSTPLAE
                     AQHARDGLIPAMAAVRAAADLLEGVVADDLWPLPTYQEMLFIL"
     gene            complement(8973..9401)
                     /locus_tag="VGD55_02995"
     CDS             complement(8973..9401)
                     /locus_tag="VGD55_02995"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0869342.1"
                     /translation="MATTRTANAHWEGPLLAGGKGTVSLDSSGLGTFDVNWRARTESP
                     EGQTSPEELIAAAHSSCFSMALSNGLAKAGHTDITLDTRADVTFQPGEGITGSHLTVR
                     ASVPGITAEEFAEIAEGARSGCPVSKALAGTTITLDAALA"
     gene            9449..9856
                     /locus_tag="VGD55_03000"
     CDS             9449..9856
                     /locus_tag="VGD55_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEY0869343.1"
                     /translation="MTRTADESFYDQVGGAETFQRLVHRFYQGVADDALLRPLYPEYD
                     LADAEVRLRMFLEQYWGGPKTYGEVRGHPRLRMRHAPFRIGPAQRDAWLQNMRVAIDE
                     LDLDPAHEQTLWDYLTAAADSLVNDLGLEDGPT"
     gene            9882..11264
                     /locus_tag="VGD55_03005"
     CDS             9882..11264
                     /locus_tag="VGD55_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869344.1"
                     /translation="MASAAQVREIFRTPNFGRLYSTRQLSAVADGIFQTALASYVFFS
                     PQNATTPAKAAAAFAALLLPYSIAGPFVGVFLDRWRRQRVLVVANTVKVALVLVVAAL
                     VLANSYGVAFLVTAVVALGVNRFFLSALSAALPHLVDQKDLVTANAVSTTSGSVSTII
                     GAAIGAGIRLATGSSSVAVASIVVLSALVYAGSAAVAATMGSRLLGPDADEHETLPRT
                     PWHAVATVAHELADATVYIARRKKAAGALAAVSAHRFLFGVASLTIVLLDRHYFATQG
                     NIDGGILGLGAAIGAVGVGIVVAALITPAATDRVGKTRWITAMLVLAGIAIAAFGLPF
                     QRGLLVAGAFLLGVSAQGVKICVDTTLQEQIGDQYRGRVFSVYDMAFNVTYVAAAAVA
                     ATVLPKSGKSFPVMAGVAGAYVLAALAFATFSAESQDNNPNHEERAFSDLQTPRRPVT
                     IKTGKPSSGA"
     gene            11330..>12359
                     /locus_tag="VGD55_03010"
     CDS             11330..>12359
                     /locus_tag="VGD55_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0869345.1"
                     /translation="MEPTAPTAAASMLADLPLPELPTDFETLVDGLESRLAGQTSDLV
                     RMRKNAQTSQALLDLHTACTRQRTVAGTARQIAAVASAITGSQIVIVSVFQVANQQPL
                     LAVSLGDGLVDEEQIGDLVCDMAGQPDMGWLRTENRLRVITRETASPSTALLLAELHA
                     ERILITPLFGQGEFVGCIVAKLPNTAPERDDIGVIEGSAALQDHAGDLIHLALVLDEL
                     RRRALHDAVTGLPNLSVYEQRLVRALARRAADQPGHSEVAAILVQVVQLGRITEIHGA
                     TGVQELLREIANRLSELAPEHDLGHLGASHFALIAHGDPGAGIDLARTVIDALDEPFE
                     VSFGSLRVF"
BASE COUNT         2069 a         4192 c         4001 g         2097 t
ORIGIN      
        1 gacgagctgg cgcgtccggt cctgctcgcg tcatggactc gccacgcggt gagtgagctg
       61 accctgcatg acctggcgac gggcgagcgc atcggtgagg tgccgctgcc gagccttgga
      121 tcggtcggcg gcttgtcaga gcgccccgaa ggcggccctg agtgctggtt cggctacacc
      181 gaccacacga ctcccgcgag cgtgttccgc tacgacgcgc gcactggtgc gacgacgtcg
      241 tgggcgaacg cacccggcat cgtcgaagtg cccaaggttc actcgcggct tgtcacctac
      301 acgtcgcgcg acggcacgcc ggtgcgcatg atcgtggtct cgacagacgc agaacctgac
      361 cacccgagac ccacaattct tggcggatac gggggtttcg gcgcatcgat gacgccgggc
      421 tactccgcga cgatgctcgc gtgggtcgaa gccggcggca tctacgcggt cgcgaacctg
      481 cgcggcggcg gcgaagaagg cgagagctgg caccgggccg gcatgcgcgg caacaaacag
      541 aacgtgttcg acgacttcca cgccgccgcc gactggctcg tcgcagccgg ctacaccacg
      601 ccgtcacaac tcggcatctc cgggggcagc aacggtggcc tgctcgtagg tgccgcgctg
      661 acgcaggcgc ctgagaagta cgcggctgtc gcgtgttcgg caccgctgct cgacatggtg
      721 cgctacgaga agttcggcct cggccaactg tggagcgaag agtacggctc cgccgaagat
      781 cccgaagaac tcggctggct gatcgcgtac tcgccctacc accacgtcgt cgagggcgat
      841 gcctatcccg cggtgttgtt cacggtcttc gattccgaca cccgcgtcga cccgttgcac
      901 gcgcgcaaga tgtgcgccgc acttcaggcc gcgacgtcag cgagccgacc gatcgtgttt
      961 cggcgcgaga gcgacgtcgg ccacggcgcc cgggctgtca gtcgaagtgt cgatctgtct
     1021 gccgacactc tggcgtttct cggcgcgcac accggtctcg cgttcgggga ctagtcgctc
     1081 tgcgtcgcgc gcgtaccctt gtgccgccaa aaccccccgt acgtgcggac ctcggcctag
     1141 tccgcagggc cgagccggat caccatcgcg gctccgccgt tcggcgacga gccgacctcg
     1201 aagctgccgc ccgacccttc ggcggtccga cgcacgatgt cgagccccag gccagtcgat
     1261 ccgcccgcgc ttcggcctct gtcgatcgat cccgcgccga tgccaggacc gtcatcggag
     1321 acggtaatcc acgcactggt tcgatcgacc tccaccgcca ccgacatcgc agtgccctcg
     1381 ggcgtgtgtg cgaacacgtt ctcgagcagt gcatcgacag ccgctgcgag gtcgtcggcc
     1441 gagacccgca cgaacctcga cgtcgtgggc agacgcaact caacccggcg tccctgatct
     1501 tctgcgagca cggcccaaaa cgccacgcgc cccttcacca cgctgacgac gtcgcacgcc
     1561 ggtgccggct gctcccgcac gccccggcgc gcctccctga tgacggagcc gacggttcgc
     1621 tcgagggcat cgacatgttc ggacagcttc tctgcctcgg gtgagtcggg caacgcttcg
     1681 accgccaact tcaccgcagc aaccggcgtg cgcagccggt gcgacaggtc ggcgacctgc
     1741 tcgcgctcgt cgtcgagcag ctcggtgatg cgctgggcga ggcgattcat cgctacgccg
     1801 acctcaacga cctcccgcgg tccgatgacg ggcgcgcgcg cctgcaactg gcccgacgac
     1861 aaccggttcg cggtcgaagc caacgccgcg acgggccgca ctgtccattg cgcgagccgg
     1921 tcggcgatca ccatcgccac cgccaggatc gcgatgccga caagtaccag gatcacgatc
     1981 gcggagaaca cacccttgtc aaaaagggca gcagacacaa aggtccggac gacgatgggt
     2041 ttgcctgccg tgtcggtgac cgggtgccag atttcgagcc cgcccttgta ccgcacctgg
     2101 acctcgcgaa gttcggtgcc gttcgggcct acggccaaat gcagcgccgg cgagtcggga
     2161 tcggctggct gcgttccgac ccacacccca tctcccagcc gcacggagac ggagattccg
     2221 gttggggaac cggtgacggc ttgggccaat gccgcttgtg ttcgctcgcc cgtttgcacc
     2281 gcggtcacga cgcgctgaga ctccttcaac ccgtcgttga gtgcacgctg gtgcgcgagg
     2341 ttgcggacca gcaccgacaa cggcacaagg aacgccacga tgacgagcgt cgtggtggcc
     2401 gccgccagga tcaggagcct gttgcgcacg tgacgggccc gccgggctaa ctgtcaggct
     2461 cggtcgggcc gacgagtttg acgccgacac ctcgcacgct gagcaggtaa cgcggcgtcg
     2521 cagccgactc gccgagcttt cgccgcaacc acgacagatg gacgtcgacg gtcttgtcgg
     2581 cgccgccgta cgcctgctgc cacacctcgg cgagcaacac gcgcttgctc acgacctcgc
     2641 cctcgtgctc ggctagatag agcaacaggt cgaactcctt gcgggtgagt tcgagcttct
     2701 cgccgtcgag gctcgcttcc ctggtgcgct ggtcgatgcg cagcccgccg acgacgaccg
     2761 cttgggcggc gtcaccgctg gccatgcgcc gcagcaccgc gcggatgcgg gcctcgagat
     2821 ggtcggaggc gaacggcttg acgacgtagt cgtcagcgcc ccagtcgagg atgcgcacca
     2881 tctcggcctg atcgtcgcgc gcggtggcga cgatgatcgg caccttgctg acggcgcgca
     2941 ccatctggat caaggtgcca ccgtcgaggt cgggcaaccc gaggtcgagt acgacgaggt
     3001 caggggccgt gtcgaccacg gcctgaaggc ccgccatggc ggtgggtgca gacgcgaccg
     3061 cgtggccgcg ctcactgagc gatcgggtca gtgaggtacg gatcgcggcg tcgtcttcga
     3121 tgagcagcag gcggggcacg tgactgacgg tactcaatcg cgcggcgaac gggcaccgac
     3181 agtgccgaga atgccgcacc accttgaggg cgctttaact ccggacgcac cccaaaccgc
     3241 cgagaatgag gaggtgtccg aagagcacga tcccgaccaa ttgctcccgc caagccgtga
     3301 ctcaagcacc aaagagtcac cgcggcggca cttttggttg accagcgaag gtggccgacg
     3361 tggagccgga cggccgctga ccctcgcttt ggggtggttg ctctcctgcg tcgtgatcac
     3421 ggcagtgacg ctgaccgcgg ttgaccgggt aggccgagag gtcagcggca gccaaaacct
     3481 ggttaccact caacagttcg tcaaagcgca ggtcgcccag cagtcggcgt ccgctgcatc
     3541 tgcaagctct gcctcaccgt ttctggcgca acctgtcatc accccaacgg tggcgccgac
     3601 gaagctgccg acgacgtcgc cgagcccgtc acattcaagt ccgaaggctt cggtttccgt
     3661 gacgcctgcg gtgaagacga ccccaccccc ggtgtcaccg tcaccatcag cctcgcttcc
     3721 gaccccgccg ggaatcgagt acgtgtacac ctgcgtcgat ggcaaggcgg tgggcggcgc
     3781 agtcactgct tcttccaccc ccacgccaac gccgacgccg agcggatctt cgacaccgac
     3841 cagctcaccc tcgtcgtcca tctcgacgtc accgtcacct tcagcttctg gcgcctcgac
     3901 atcgagttcg cccacaccca gcccgtcgac aggcaagatc atcgagacgg tgccgggcgg
     3961 gcggatcgtg atcgcgtgcc tgggcgacac gatcgcgaac gcgagcgcga cgaccacgga
     4021 tcccggctac acgctcacgc cgcgatcgct ggtcgacccg cgcgtcgacg tcttcttcag
     4081 caagaactag ccgcgcacct ctcgccgtcg ccggtcgcgt cgcccgcccg gtgaccccgt
     4141 cccggccact tcacatccct tgtggttgct gagccagccg atttgtcgcg tttgggcagc
     4201 cactggggat gtgaagtggt gcctccgcgg ggctccggtc gctgcgttga cagtggcgca
     4261 gcgtccgcac tagcgtggta ggcagggaag ttaacaatcg gacactgccg aggacccacc
     4321 caccgctgcc cgtggagacg cttgtgaagc ttgccgttgt cggtggcggt tcgacctaca
     4381 cgcccgaatt ggtcgacggc atggcgcgct tgcgcgacca gctaccgttg gaggagctgg
     4441 tcctggtgga ccccgacccg caccgactcg aactggtcgg cggcatctcg aggcgcatca
     4501 tggcggcgca ggatcaccct gcgaaggtgt cctggacttc tgacctcgac gccggcatcg
     4561 actcggccga cgcggtggtg ctccagctac gcgtgggcgg ccaggctgcg aggcacaagg
     4621 acgagagctg gccacttgaa tgcgggtgcg tcggccagga gacgaccgga gccgggggtc
     4681 tggcgaaagc gctgcgcacg atcccggtcg tgctcgacat cgccgagcgc gccgagaagc
     4741 acgcggcgcc cggtgcctgg atcgtcgact tcaccaaccc ggtcggcatc gtcacccggg
     4801 cgttgctcga cggcgggcac aaagcagtcg gtctgtgcaa cgtcgcgatc ggccttcagc
     4861 gctacttcgc gcagttgctc ggcatcgacc cgacgaccgt ccacctcgac cacgtcggcc
     4921 tcaaccacct gacgtgggag cgcgcagtca ccgtcaacgg ggtcgacgtg ctcccccaac
     4981 tgcttgccga ccacggcgac gaaatcgcta gccacatcga acttccgctg gccgtattac
     5041 gccagctcgg cgtcgtcccg tcgtattacc tgcggtacta ctacgagcac gacaacgtcg
     5101 tcgacgaact gcgcgggcag ccgaccaggg cgcaggtcgt cgccggggtc gagcaggagt
     5161 tgctcgacat ctacgccgat ccgaccgtgt cgactaagcc cgcgcttctc gaacaacgcg
     5221 gcggtgcgta ctactccgag gccgctgtga acttgatctc ttccctcgag cgcgacaccg
     5281 gcgacgttca ggtcgtgaac gtgcgcaatg ccggcacgct gccgttcctg gccgacgacg
     5341 ccgtcatcga ggtgccggcg atagtcggcg cagacggcgc caaacccctc cccattgccc
     5401 aggtcgatcc gctctacgcg ggcctcattg cgcacgtgac ggcttacgaa gaactcgccg
     5461 tcgacgcagc gatccacggc ggccgcgacc gcgtcttcaa ggcgctgctc gcgcatccgc
     5521 tggtcggcca gatcgatctc gcgaatggtc tcaccgatcg cttgctggcc gcgaactacg
     5581 agcacctgcc ctgggtgagc aagagcacca gccggtgaac gacggcccgc ccgtcgccgt
     5641 cctcgcaatc gacggcggca acagcaagac ggacgtcgcg ttggtgagcg tcgaaggtga
     5701 cctgctcggc cactcgcgtg gtcgccctgc gctcgcgccc gagatcggca tggaaccggc
     5761 gctgcggatc atcagcgggc tggtcgacga agccgcacag gacgctggtc tgactcgcga
     5821 tgccggccct ctcgcgcagc acacgtcggc ctacctcgcg ggcgtcgaca tcccgcagga
     5881 gcaggtagcg atgcaccaag cgctgctcga gcaagggtgg agcagcaccg tccgcgtcga
     5941 caacgacacg ttcgcaatct ttcgagccgg caccacccag cactggggtg ttggggtcgt
     6001 ttccggcgcg ggcatgaatg ccgtcgggat cggtcgcgac ggtcgagtcg gccgctacct
     6061 cgcgctcggt gagttcaccg gtgacttcgg cggtgggaac gacctgtcgt accaggtcat
     6121 gtactggacc tttcgcgacg aagacgggcg cggtggcgaa accgcgctga cgccggcagt
     6181 gtgcgagcac ttcgggctgc cgaccatcta cgacgtggcg ttggcgatgc atcgcggcga
     6241 gatcaccaag acgcagcgcc tcgagctcac ccgggtgctg ttcgaggttg ccgccacgac
     6301 cgccgacccc gtcgcggtgt cgcttctcga acgtcaggcg cacgaggtcg cgctgatggc
     6361 tcggcaattg ctgcgcgacc tcgacctgat cgacgtcgag accgacatcg ttctcggagg
     6421 cggcgtcctg accagtcgcg acccgctgtt gacgcgactg atccgccaat cgatggcagc
     6481 cgaggcaccg aaagggcgcg tgacaatcaa cgaggtgccg gcggtcaccg gtgccgcgct
     6541 actcggcttc gaccacctcg gcctcggacg tacagcggag gatcgcctgc gttcgcagat
     6601 gcgatccacc gccgacacat ccgccgtaaa gaatgcggtg tcgacgcaca gaatgctgca
     6661 tcgattcaca aaatgaaaag catctcctga taggtcggca gcggccacaa gtcgtcggcc
     6721 accacaccct cgagcaaatc ggctgcggcc cgtacagcgg ccatcgctgg gataagcccg
     6781 tcgcgggcat gctgcgcctc tgccagcggc gtcgagccgc tgtcgggggc tagctcgtcg
     6841 cgcaagatgc cgagcgctgc ccgcagcgcc ttcagcggtg ccgagacgtc gtcgagcgtc
     6901 gacgggtcga cctcgacacc agccgccttc agcgcgccga ggttgagcgc gacctccgtc
     6961 tggtagcgca ccgctgccgg caacacgagc gtcgagccga tctcgagggt caagcgcgcc
     7021 tcgacaccga cactgagcgc gtactgctcc aggccgatct cgtagcgact gtgcatctcg
     7081 cgatgggtga agacgccgta cttgctgaac aactccatcg acggctccga gatgagctcg
     7141 ggcaacgcgt cgagtgtcgt gcgcaggttc ggcaagccgc gctgggccgc ctcgacctgc
     7201 cactgctctg aatagccgtc gccgttgaac accgacgcgc cgtgcacggt gatgatctct
     7261 tcgaggatct tctgcaccgc gacgttgaac tcggtgcccg aagccagcgc ggtctcaaga
     7321 ctcgtcgcga tgtagtcgag cgcctcggcc atgatcgtgt tgatcgtcac catcggcccc
     7381 gccaccgact gcagtgaccc gggtgctctg aactcgaacc ggttgccggt gaaggcgaac
     7441 gggctggtgc ggttgcggtc gcccgggtcg gtcgggaggt tgggcagcgt gtcgacgccg
     7501 atcgtgagcg tgcccttttc cttggaggac gtcgcgcccc ctttggcgat ctgatcgaag
     7561 acgtcggtca gctggtcgcc gaggaagatc gagatgatgg ccggtggcgc ctcgttggcg
     7621 ccgagacggt ggtcgttgga cgccgatgcc accgacgccc gcagcaggcc accgtacttg
     7681 tgcaccgcgc ggatcacggc tgcgcagaac accaagaact gcgcgttctc gtgaggcgtg
     7741 tcgcccggca gcagcaggtt gccctcggtg gagttgccga gggagaagtt gacgtgcttg
     7801 cccgagccgt tgattcccag gaatggtttc tcgtggaaca agcactccat gccgtgcttt
     7861 tgcgcgaggc gcttgaacgt cgacatcaac agttgctgat ggtcggcggc aacattggcg
     7921 cgttcgaaca tcggggcgat ctcgaattgg ccgggcgcga cctcgttgtg gcgggtcttg
     7981 gccgggatgc cgagcttgaa cagctcacgc tcggtatcca tcatgaaccc cagtacccgc
     8041 tcggggatcg cgccgaagta atggtcgtcg aactcctgac ccttgggcgg cttcgacccg
     8101 aagagcgtgc ggccggcgtt caccaagtcg ggcctggcca ggaagaagtg ccggtcgatg
     8161 aggaagtact cctgctcggc accgcaaaac gagacgacgt ggtcggggtg tgcgtggccg
     8221 aacagcttca agattcgctc ggcgtgcacc gccatcgact gctgtgagcg cagcaaaggc
     8281 gtcttgtggt caagagcgtc accggtcatc gatacgaaga tcgtcgggat gcacagcgtg
     8341 ttcccgttcg ggttctccag cacgtaggcc gggctcgtga cgtcccaccc ggtgtagccg
     8401 cgagcctcga acgtgttgcg caggccacca ctcgggaagc tcgaagcgtc cggctcaccc
     8461 tgcgtcagcg tcttgccggc gaactcggcc agtgcagacc cgtcgcccgc gggctccagg
     8521 aagctgtcgt gcttctcggc ggtcagaccg gtgaggggat agaagacgtg cgcgtagtgc
     8581 gtcgcgccct tctccatcgc ccaatccttc atcgcggcgg caactgcgtc ggccacgagc
     8641 gggtcgagcg gcgccgaatg ctcgatcgtt gccatcaccg acttgaagac cgacttgggc
     8701 agccgctgtt gcatcacggt tttgctgaag acgtttgcgc cgaagatctc acccggcgcc
     8761 tcactggcca ggtaactgac ggccggcgga acgtaggcct cgacgtcttt gatcgcctgg
     8821 aggcgaacgg catttccact catagaggtc cccttgggtg gcgcttgcga accgctgctc
     8881 cagcggctgt caacgcagcg accctaggga cccctcgcat cgggcgtgtt tcgaaaccgt
     8941 gaacgctgca atgcgttaac ggcgatcacg acttacgcca gggcggcgtc cagcgtgatc
     9001 gtcgtccctg caagtgcctt gctcaccggg cacccgctgc gcgccccttc ggcaatctca
     9061 gcaaactcct cggccgtgat gccaggaacg ctcgcacgca cggtgaggtg gcttccggtg
     9121 atgccctcac ccggctggaa ggtcacgtcg gcgcgggtgt cgagcgtgat gtcggtgtga
     9181 cccgcctttg ccagcccgtt cgacagcgcc atcgagaagc acgacgagtg ggcggcggcg
     9241 atgagctcct ctggactcgt ctgaccttcg ggtgactccg tacgcgcccg ccagttgacg
     9301 tcgaacgtgc ccagccccga cgagtcaagg gagaccgtgc cctttccccc ggcgagcagt
     9361 gggccctccc agtgagcgtt ggcggtgcgg gtggtggcca taagtggtcc ttccgtgcag
     9421 tggacgacaa ttggacgaca atgcacatgt gacgcgaacg gcggacgaga gtttctacga
     9481 ccaggtcggc ggtgccgaga cctttcagcg cctcgtccat cgtttctatc agggcgtcgc
     9541 cgacgacgcg ttgctccgcc ccctctatcc agaatatgac ttggcggacg ccgaggtgcg
     9601 actgcggatg ttcctcgagc agtactgggg cggaccgaag acctacggcg aggtgcgcgg
     9661 ccacccaagg ctgcggatgc gacacgctcc gtttcggatc ggaccagcgc agcgcgatgc
     9721 gtggttgcag aacatgcggg tggcgatcga cgagctcgat cttgacccgg cgcacgagca
     9781 gacgttgtgg gactacctga ccgcggccgc cgacagcctg gtcaacgatc tcggtcttga
     9841 agacgggccc acctagcaga gcgcacgaat acgcttcgac tgtggcgagt gcagcgcagg
     9901 tccgcgagat ctttcgcaca ccgaactttg gccgcctcta cagcacccga cagttgtcag
     9961 ccgtcgccga cggcatcttc caaaccgcgc tggcgagcta cgtcttcttc tccccgcaaa
    10021 acgcgacgac tcccgcgaag gcggctgcgg cgttcgctgc cctgctcctc ccctactcca
    10081 tagccgggcc gttcgtcggc gtgttccttg atcggtggcg acgccagcgg gtgctcgtcg
    10141 tggcgaacac cgtcaaggtc gcacttgttc ttgtggtggc tgctctggtg ctcgccaact
    10201 cctacggcgt cgcgttcttg gtcacggcag tggtcgcgct cggcgtcaac cggttcttcc
    10261 tgtcggcact gtccgcggcg ttgccacatc tcgtcgacca gaaagatctg gtgaccgcca
    10321 acgcggtatc gacgacgtcg gggtcggtga gcacgatcat cggggcggcg atcggcgccg
    10381 gtatccggct cgccaccggt tcgagctcgg tcgcggtcgc gtccatcgtc gttctatccg
    10441 cgctggtcta tgccggctcc gccgcggtcg ccgcgaccat gggcagtcga ctgctcggcc
    10501 cggacgccga cgaacacgag acgctccccc gtacgccctg gcacgccgtg gcgaccgttg
    10561 cgcacgaact cgctgacgcg accgtctaca tcgcgagaag gaagaaagcg gcgggtgcct
    10621 tggcggccgt cagcgcccac cggttcctgt tcggagttgc gtcgctgacg atcgtgctgc
    10681 tggatcgcca ctacttcgcg acccagggca acatcgacgg cggcatcctc ggcctcggtg
    10741 cggcgatcgg tgccgtcgga gtcggcatcg tggtcgcggc gctgatcaca ccggcggcga
    10801 ccgaccgggt cggaaagacg aggtggatca ccgcgatgct ggtgctcgcg ggcatcgcga
    10861 tcgcggcgtt cggcctgccg ttccagcgtg ggctgctggt ggccggagcg ttcctgctcg
    10921 gtgtttcggc tcagggggta aagatttgcg tcgacacgac cctccaggag cagatcggcg
    10981 accagtaccg cggccgggtg ttctcggtct acgacatggc cttcaacgtc acctacgtag
    11041 cggctgccgc ggtcgccgcg accgtgctac cgaaatcggg caagtcgttc cccgtcatgg
    11101 ccggcgttgc cggtgcgtac gttctggcag cgctcgcatt cgcaacattt tcagccgaat
    11161 cgcaggataa taacccgaat cacgaagaac gagcgttttc agacctccaa acccctcgcc
    11221 gtccggttac gatcaaaact ggaaaaccct caagtggggc atagccagag ccgtagttcc
    11281 gaataggtac gcatccgtcg ggtgccggat gaggtggagg tatttggctg tggagccgac
    11341 cgcgccgaca gccgccgcat cgatgcttgc tgacttgccg ctgcccgagt tgccgaccga
    11401 cttcgagaca ctcgtcgacg gccttgagag caggctggcc ggtcagacct ccgacctggt
    11461 ccgcatgcgc aagaacgcgc agacctccca agccttgctc gacctgcaca ccgcctgcac
    11521 ccgccagcgc acggtcgccg gcaccgcgag gcagatcgcc gctgtggcct cggccatcac
    11581 aggctcgcag atcgtcatcg tcagcgtctt ccaggtggcc aatcagcaac cgctgctagc
    11641 ggtgagtctc ggcgacggtt tggtcgacga ggagcagatc ggcgacttgg tgtgtgacat
    11701 ggcgggccaa cccgacatgg ggtggctgcg tacagagaat cgcttgcgtg tcatcacccg
    11761 cgaaactgcg agcccctcga ccgctttgct gctcgccgaa ctacatgccg aacggatcct
    11821 gatcacccca ctgttcgggc agggcgagtt cgtcggctgc atcgtcgcca aactgcccaa
    11881 cactgcgccc gaacgcgacg acatcggcgt gatcgaagga tctgccgcgt tgcaggatca
    11941 cgcgggcgac ctgatccacc tcgctctggt gctcgacgag ttgcgcaggc gggctctgca
    12001 cgacgcggtg acgggcctgc ccaacctctc ggtgtacgaa cagcgactcg tcagagcact
    12061 cgcccggcgc gccgccgacc agccggggca ctccgaagtg gccgcgattc tcgtgcaggt
    12121 cgttcagctc ggccgcatca ccgaaataca cggcgcaacc ggtgtacaag agctccttcg
    12181 cgagatcgcg aaccgcctga gtgagcttgc tcctgagcac gaccttggtc acctcggcgc
    12241 gagccacttc gcgctgatcg cccacggcga tccaggcgcc ggtatcgacc tggctcgcac
    12301 cgtcatcgac gcccttgatg agcctttcga ggtcagcttc ggcagcctgc gcgtctttt
//
LOCUS       DASWVF010000174         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_488839, whole genome shotgun sequence.
ACCESSION   DASWVF010000174 DASWVF010000000
VERSION     DASWVF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488839"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VGD55_03015"
     CDS             complement(<1..613)
                     /locus_tag="VGD55_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0869346.1"
                     /translation="MGTWAEDPELLATFRAEVDERLASLSAGLLQLESNGAPRQLIAA
                     LFRDAHTVKGSARMMGLATVLETAHAAEDLLGALRDGRFQVKREYVDLLLATVDGIGR
                     SIATAPNTAVDAYLGELVDALRGALEGDDPVKVPRMSQPVPVASADPPVAAAARVSRA
                     RAKGASHSGASQSGAAGGESAAQAPADPITAPLGVPATGSVPFI"
     gene            complement(620..>2135)
                     /locus_tag="VGD55_03020"
     CDS             complement(620..>2135)
                     /locus_tag="VGD55_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0869347.1"
                     /translation="ALGAAVITAISLTDLQHPLLQPTGLIVDLGRLLYLGGVTGVLVA
                     AHGIATPLWVLFLPVLLTTAFVDVQWKALAYAVGASGLTALAVGITHGFAKASAVPIL
                     LILAAMPAVVAATSTSAQSINALAGLAETERIRQAKEVEELREQLIEGHRAERETLLD
                     ETRRQRAELKVKVDRLSESLASAARGDLSGYSDGAWAAATSDGDETLASLQSAFNNTL
                     GNLRLLVEQIRGSGESISASAGELLSTAEEHAASATQQSSAVAETTSTIEELAATAAQ
                     IAETSEAVARYAAETLRFAEEGREAVAASVAAMDTIAHRVDSIATRALSLGAKSQEIG
                     RILEVIDDLADQTNLLALNAAIEAARAGEHGRGFAVVASEVRKLAERSQESAAQIQGI
                     VSEIQAETNATIIATEEGAKEVHQGTELARGVVDALERISGMVDETTTAAKEISIATQ
                     QQRSASDQVVTAMMQVSDVSRQYVVGSKQAAASASQLNVLAAELRASIAQFRVA"
BASE COUNT          353 a          711 c          711 g          360 t
ORIGIN      
        1 cgatgaaagg aacactgcca gtagcgggga cgccgagggg tgccgtgatc gggtcagccg
       61 gcgcttgtgc cgcgctctcg cccccagctg caccgctctg cgacgcacca ctgtgcgaag
      121 cgcctttcgc gcgggccctc gagacgcgcg cagcggcagc aactggcggg tcagcggacg
      181 cgactggcac cggctgcgac atccgcggca ccttcaccgg gtcatcgcct tccagtgcac
      241 cgcgcagcgc gtcgaccagt tcgcccaggt acgcatcgac tgccgtgttg ggggcagtcg
      301 ctatcgagcg gccgatgccg tcgacagtcg cgagcagcag atcgacgtac tcacgtttca
      361 cctggaagcg gccgtcgcgc agcgcgccga gcagatcttc ggccgcatgc gcagtctcca
      421 acaccgtggc gaggcccatc atgcgagcgg aacccttgac ggtgtgtgcg tcgcggaaca
      481 gcgctgcgat caactgccgc ggcgcgccgt tcgattcgag ctgaagcaac cctgcgctca
      541 gcgacgcgag gcgttcgtcg acctcggcac ggaaagtcgc gaggagttcg ggatcctcgg
      601 cccaggtgcc catcgcgggc taggccactc ggaactgggc aatcgacgcc cgcagttccg
      661 cggcgagcac gttgagctgc gacgccgacg ccgcagcctg cttggagccg accacgtact
      721 gacgtgagac gtcagagact tgcatcatcg ccgtcacgac ctggtcggac gcagagcgct
      781 gttgctgggt cgcaatcgag atttcctttg cggccgtggt cgtttcgtcg accatgccag
      841 agatccgctc gagcgcgtcg acaacgcccc gggccagttc ggtgccctgg tgtacttcct
      901 tggcaccttc ctcggtcgcg atgatcgtcg cgttggtctc ggcctgaatc tcactcacga
      961 tgccctgaat ctgcgcggcc gattcctgcg agcgttcagc cagcttgcga acctcggacg
     1021 cgacgaccgc gaatccgcga ccgtgctcac cggcacgggc cgcctcgatc gcagcgttca
     1081 gcgcgagcag gttggtctgg tcggcgagat catcgatcac ttcgagaatg cgaccgatct
     1141 cttgtgactt cgcgccgagt gacaatgcgc gagtcgcgat cgagtcgacg cggtgcgcga
     1201 tcgtgtccat cgcggcaacc gatgctgcca cggcttcgcg accctcttcg gcaaagcgca
     1261 acgtctcggc ggcgtagcgc gcgacagctt ctgacgtctc tgcgatctgg gccgcggtgg
     1321 ccgcaagctc ttcgattgtc gaggtggtct cggcgacggc agaagactgc tgcgtcgccg
     1381 acgcggcgtg ctcttcggca gtgctcagaa gctcacccgc agacgccgag atgctctcgc
     1441 cgctgccacg gatctgctcg acgagcaggc gcaagttgcc cagcgtgttg ttgaacgcgg
     1501 attgcaacga tgcgagcgtc tcgtcgccgt cggaggtcgc agcggcccac gcgccatcgc
     1561 tgtaaccgga gaggtcaccg cgagcggcac tggccaatga ctcggacagc cggtcgacct
     1621 tcaccttgag ttccgcacgc tgccgacgag tctcgtcgag cagcgtctcg cgctcggcgc
     1681 gatgcccctc gatcagttgc tcgcgcagtt cctcgacttc cttcgcctgg cggatccgct
     1741 cggtttcggc caaccccgcg agggcgttga tcgactgtgc ggacgtgctc gtcgccgcca
     1801 ccactgctgg catcgctgcc aggatcagca gaatcggtac ggccgacgcc ttggcgaagc
     1861 cgtgggtgat gccgaccgcg agcgcagtaa gaccgctagc gccaaccgcg taggcaaggg
     1921 ctttccattg gacgtcgacg aaggcggtcg tgagcagcac tggaaggaag agcacccata
     1981 gcggcgtcgc gatgccgtgg gcggcaacga gaacaccagt cacgccgccg aggtagagca
     2041 gccgtccgag gtcgacgatc aggccggtcg gctggagaag ggggtgctgc aagtccgtga
     2101 gcgagatcgc ggtgatgacg gctgccccaa gggcg
//
LOCUS       DASWVF010000175         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_412569, whole genome shotgun sequence.
ACCESSION   DASWVF010000175 DASWVF010000000
VERSION     DASWVF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412569"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..243)
                     /locus_tag="VGD55_03025"
     tRNA            complement(171..243)
                     /locus_tag="VGD55_03025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Arg,seq:cct)
     gene            401..679
                     /locus_tag="VGD55_03030"
     CDS             401..679
                     /locus_tag="VGD55_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869348.1"
                     /translation="MEQAEVVAFPARGEVFVDHRGQSRALRLAWHIEADVVVLSLWQA
                     DRCSGTFRLAIDDVPRFVAALVEGLGAAATLPAAQAARLRMARTSSAI"
     gene            complement(688..1392)
                     /locus_tag="VGD55_03035"
     CDS             complement(688..1392)
                     /locus_tag="VGD55_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY0869349.1"
                     /translation="MKVEIWSDVVCPWCYVGKRRFEAALASFEHRADVEVHWRSFELD
                     PSAPAVREGDPVQRLADKYGMTRAEALAADARLTDLAAVEGLDFHLETARSGNTFDAH
                     RLLHLAAGKGVQDAVKERFMRAYFTESEPVGEHATLARLAVDAGLDETDVNAVLTSDR
                     YASDVRADEQQAAAYGISGVPFFVIDARYGISGAQSSDVILATLRTAYAEANPLTMVT
                     ADVTVDDSCEGDACAV"
     gene            complement(1432..>1621)
                     /locus_tag="VGD55_03040"
     CDS             complement(1432..>1621)
                     /locus_tag="VGD55_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869350.1"
                     /translation="IGLLVALAIVATIVMVSRNRPPQPERKHPDPYRVRGRSMRGWQA
                     SALREIRRAGRQHGTRGK"
BASE COUNT          260 a          538 c          528 g          295 t
ORIGIN      
        1 gacggaagtg cgctgatcgc ggcgtacaac ttcaatacgc cgttcagctt caccgagctc
       61 aacctgcgcg cgccgcgcta tctacacatc gggatgccat agaagacgcg gaggtcgggc
      121 ggggaggtcg tccgcaggtt cagaggtttc cgatgtcttg agatgacaag tgccctcggc
      181 aggattcgga cctgcgacac ccggtttagg aaaccggtgc tctatcccct gagctacgag
      241 ggcgggggca gcgacagcgc tgcgtttgga ggctagcgga cgtcgccggc cggcccgaat
      301 ccccgcgtct cgggttcgac acgagcggct gtcgccttga tcagacagga ctactccctt
      361 gggtcttccc ggccgcgcct gcggcggcta acgtcagccc atggagcagg ccgaagtggt
      421 cgctttcccg gcgcgtggcg aggttttcgt cgaccatcgc ggacagtccc gtgcgttgcg
      481 ccttgcctgg catatcgagg cggacgtcgt ggtgctcagc ctctggcagg ccgaccgttg
      541 ctcaggaacg tttcgcctag ccatcgatga cgtgccgcgg tttgtggctg cactcgtcga
      601 aggtctcgga gccgcggcga cgttgccggc agcccaagca gcgcgcttgc ggatggctcg
      661 cacgtcgagc gcgatttaac ccaagctcta caccgcgcag gcgtcgcctt cgcagctgtc
      721 gtccacggtc acgtcagccg tcaccatcgt cagaggattc gcttcggcat acgcagtacg
      781 cagtgttgcg aggatcacgt cgctcgactg cgcgcctgaa atgccgtaac gcgcgtcgat
      841 gacgaagaac ggaacaccgg agatcccata cgcagcagcc tgttgttcgt ctgcgcggac
      901 gtcggacgcg taccggtcgg aggtcagcac cgcgttcaca tcggtttcgt cgagccctgc
      961 gtcgaccgcg agtcgcgcca gcgttgcatg ctcgccgacg ggttcgctct cggtgaagta
     1021 cgcgcgcatg aagcgttcct tcacggcgtc ttgaacgccc ttgccggcag cgaggtgcaa
     1081 tagccgatgc gcgtcgaagg tatttcccga gcgggccgtc tccagatgaa agtcgagacc
     1141 ctcgaccgcc gccaggtcgg ttaacctcgc atcggccgcg agcgcttccg cgcgcgtcat
     1201 tccgtacttg tctgcaagac gttgcaccgg atcgccctcg cgaaccgccg gcgcagatgg
     1261 gtcgagctcg aagctacgcc agtgcacctc gacgtccgcc cgatgttcga agctcgctag
     1321 tgcggcctcg aagcggcgct tgccgacgta acaccagggg cacacgacgt ccgaccagat
     1381 ttctaccttc atacgggctc aaactttgcc ggacgccgcg ttgttccgcc gctacttccc
     1441 gcgggtgccg tgctggcggc cggcccggcg gatctcgcga agagccgacg cctgccagcc
     1501 ccgcatgctc cggccgcgga cgcggtacgg gtcggggtgc ttgcgctcgg gttggggcgg
     1561 ccggttgcgg ctcaccatca cgatcgtcgc gacgatggcc agcgccacca ggagcccgat
     1621 c
//
LOCUS       DASWVF010000176         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_335966, whole genome shotgun sequence.
ACCESSION   DASWVF010000176 DASWVF010000000
VERSION     DASWVF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335966"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1325)
                     /locus_tag="VGD55_03045"
     CDS             complement(36..1325)
                     /locus_tag="VGD55_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0869351.1"
                     /translation="MRKTRSDVEQQVADLLLIAKKSLGLSVSFLSRLDGVTQHLEVVE
                     SSVPFLFQEGNKQVQATSLCQAILEKKLPAIIPDLKAFPAAMKLPAARLPHIRSYVSA
                     PVVLSDGTLYGTFCAAGLTSDKDLTKRDKSLMDVLAHAAAVIIEPGVRDRARHAEIAQ
                     RLAPILSSGGPAVVLQPIVELATGRRIGAEALSRFPAEWGKPPDICFEEAHSIGEGDR
                     LELLALQRAADYLDEVTGYVAMNVSPATLLTPACAELLTQFKLDRVVLELSEHDQVAD
                     YDDLRTVLEPLRAEGMRLAIDDVGAGFSSLRHIILTTPDVIKLDRSIVDGVSTDPVLT
                     TLVRSLVDFARGCDARVVAEGVETAADGEILLGLGVDYGQGWFYGRPGPAADLAAAAP
                     GTVGGAGHSGRHDELSARSSTERPFVARSSQRDLSST"
     gene            complement(1382..2587)
                     /locus_tag="VGD55_03050"
     CDS             complement(1382..2587)
                     /locus_tag="VGD55_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0869352.1"
                     /translation="MSTPSGATQDEIRRRNVSTLLRQVHVQGQLSRAQLTTRMGLNRS
                     TIKALVGDLCDAGLVVETIPENGLRAGRPSHLVGPRSDTAYVLAANIGVDTVTVAAVG
                     LGGVINARSEYRLPGPGVDYDLVIERLADELRRLQKSVKPGGWLAGVGVGVPGAVRQA
                     DGFVEFAPNLGWRGVPFGDRLSQRLALPVRTSVGNDGDLGGLAEHVRGAGRGIGHLIY
                     LASEVGIGGGIVVDGRILTGAHGYGGELGHMMVNPDGRLCRCGSRGCFETEAGEDAVL
                     VACGRTAGGGRAALMEVFAAASHGEKRALDGLQSIALWVARGLTSLVNILNPDVVILG
                     GPLSSLFFLTGDRIKSEMDAMSTFAGRAQVRLLQPGLGEDSSLLGAAELAFEPLLDDP
                     VDAWSRRAS"
     gene            complement(2636..4117)
                     /locus_tag="VGD55_03055"
     CDS             complement(2636..4117)
                     /locus_tag="VGD55_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869353.1"
                     /translation="MSEQSTGAPGSEAGPSAADLPITNVITTPISDDAVAEAFSGDLG
                     SLTLMDSARAYVSKVRGGDPGALPALLGLVILLVAFSSLSNQFLTVGNLANFTGQAGY
                     TILIAMGLVFVLLLGEIDLSAGTAAGVCAMMMGLAYAHNGNIEGYLKGGSYGAIVIVM
                     VIGLLIAVLNRLWLAVPMIVLGLVVILTHVGGHVWIAMFLALAVGTTIGCITGFLVAR
                     VGIPSFIVTLAFFLAWQGVIISFAGKAGAISIGNGPINELVHSNASPTLGWIFLVVFV
                     GGYAAFTLFRSIRRRAQKLSAEPLALVLVRIAGLVIVGVFAVYLLNRNRQVNKLGKAL
                     IGMPWVIPLIMVLLVILTVLLTKTRFGRYLYAVGGNAEAARRAGIDVRRMRIAAFALS
                     TTMAAVGGIALASFDGGVPLDIGGGNTLLYAVGAAVVGGVSLFGGRGRIRDAVIGGTV
                     IAMSPNGLGLKPGVNAAYTYVITGAFLLLAASVDAFGRRRSAK"
     gene            complement(4125..>4542)
                     /locus_tag="VGD55_03060"
     CDS             complement(4125..>4542)
                     /locus_tag="VGD55_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0869354.1"
                     /translation="RSRDTLAGLSVRTVKSVRQQVSSLSGGQRQSVAIAKAVLWNNKV
                     VLLDEPTAALGVAQTRQVLDLVRRLADSGLGVVLISHNMNDVMEVADRITALFLGRVA
                     AEVKRVEVTYSQVVELITAGRTGSIGLQPATAAESA"
BASE COUNT          858 a         1473 c         1492 g          719 t
ORIGIN      
        1 gtagctcgct ccaccacgcg gtagccgcgg tgaggtcagg tgctggatag atcacgctgc
       61 gaagaccgtg caacgaaagg ccgttcagtt gaggaccggg cgctgagttc gtcatgtcgt
      121 ccactgtgcc ctgccccacc gacagttcct ggtgctgccg ctgcgagatc ggccgccggg
      181 ccaggtcggc cgtaaaacca gccctggccg tagtcgacgc cgagccccag caggatctcg
      241 ccgtcggcgg cggtctcgac accctcggcg acaactcgag cgtcgcagcc gcgggcgaag
      301 tcgacgagcg agcgcaccaa cgtcgtgagc accgggtcgg tgctgacgcc gtcgacgatg
      361 ctgcggtcaa gtttgatgac gtccggggtc gtcaagatga tgtggcgcag cgaggagaat
      421 ccggcgccga cgtcgtcgat ggcgagtcgc atgccctccg cgcgcagtgg ctcgagcacg
      481 gtgcgcaaat cgtcgtagtc ggcaacctga tcgtgctcgg agagttcaag cacgacacgg
      541 tcgagcttga actgcgtcag cagttcggcg cacgcggggg tcaggagggt ggcaggcgac
      601 acattcatcg cgacgtagcc ggtcacctcg tcgagatagt cggcggcgcg ttgcagcgcg
      661 agcaactcga gccggtcgcc ctcgccgatg ctgtgcgctt cctcgaagca gatatccggc
      721 ggcttgcccc actcggcggg gaagcggctg agcgcttcgg caccaatccg ccgtccggta
      781 gcgagttcga caatcggttg cagtaccacg gcggggccac ccgagctcag gatcggcgca
      841 agccgctgcg cgatctcggc atgcctcgcg cgatctcgga ctccgggctc gatgatgacc
      901 gccgcagcgt gcgcaaggac gtccatcaac gacttgtcgc gcttggtcag atccttgtca
      961 gacgtcaggc cggcagcgca gaacgtgccg tacaaagtgc cgtcgctcag cacaaccgga
     1021 gctgagacat agctgcggat gtgcggcagg cgtgcggccg gcaacttcat ggctgccggg
     1081 aaggctttga ggtcgggaat gatggcgggc agctttttct cgaggatcgc ctgacacagc
     1141 gacgtcgcct gcacctgctt gttgccctcc tggaacagga agggcaccga ggactcgaca
     1201 acctcgaggt gttgggtcac gccgtcgagt cggctcagga acgagacgct caggccgagc
     1261 gacttcttgg cgatcagcag caggtcggcg acctgttgct cgacgtccga gcgcgttttc
     1321 ctcacatggg gctatcgaca gcaccagtcg ctcggctgag gaaggagcgt gaaccgattc
     1381 gctagctggc tcgacgcgac cacgcgtcga cggggtcgtc caggaggggc tcgaaggcga
     1441 gctcggctgc acccaggagg ctcgaatcct cgccgagccc gggctggagg agccgcacct
     1501 gcgcgcgacc ggcaaacgtc gacatggcgt ccatctccga cttgatgcgg tcgccggtga
     1561 gaaagaacag tgacgaaagg ggtccgccaa ggatcaccac gtcgggattc aggatgttca
     1621 ccaggctcgt cagccctcgg gcgacccaca atgcaatgct ctggagacca tcgagtgcgc
     1681 gcttttcgcc gtgactcgcg gcggcaaaaa cctccatcaa cgccgctcgc ccgccccccg
     1741 ccgtccgccc gcatgcaacg agaactgcat cctcgcccgc ctcggtctca aagcatccgc
     1801 gactgccgca tctgcaaagg cggccgtcgg gattgaccat catgtgtccg agctcgccgc
     1861 catagccgtg ggcgccggtg aggatgcggc cgtcgacgac gatgccacca ccgatgccga
     1921 cttcgctggc gagatagatg aggtgcccga tcccacggcc ggctccgcgg acgtgctcgg
     1981 ccaggccgcc gaggtcgccg tcgttgccca cgctggtgcg aacgggcagc gcgagccgtt
     2041 gcgagagccg atcgccgaac gggacccccc gccagccaag gttgggcgcg aactcgacga
     2101 acccgtcagc ctgtcgcacg gcccccggga cgccgacgcc gacaccggct agccacccgc
     2161 ctggcttgac ggacttctgg agcctgcgca gttcgtcggc cagccgttcg atgacgaggt
     2221 cgtagtcgac gcccgggccg ggaagccgat actcgctgcg ggcattgatc accccgccga
     2281 gcccgacggc cgccaccgtc accgtgtcga cgccgatgtt ggccgcgagc acatacgccg
     2341 tgtcggatct cggaccgacc aggtgcgaag gtcgtccggc cctaagcccg ttctccggga
     2401 tcgtctcgac gacgagcccg gcatcgcaca agtcacccac aagcgccttg atcgtgctgc
     2461 ggttcaaccc catgcgggtc gtcagctgtg cgcgtgacag ctggccttgg acgtggacct
     2521 gccgcaacaa cgtgctgacg ttgcgacgcc tgatctcgtc ttgggtagct cccgatgggg
     2581 tcgacatggt ggacagcgct cggttatccg agacgacgat tgcggcgtcg tcgtgtcatt
     2641 tcgccgagcg acgtcggccg aaggcgtcaa cggatgctgc gagcaacaag aaggcgccgg
     2701 tgatgacata ggtgtacgcg gcgttgacgc cgggcttgag cccgagcccg ttggggctca
     2761 tcgcgatcac cgtgccgccg atgaccgcat cacgaatccg gccacggcct ccgaagaggg
     2821 agacaccacc cacgacggct gcaccaacgg cgtagagcag cgtgttgccg ccaccgatgt
     2881 cgagcggcac accgccgtcg aaggaggcca atgcgatacc accgacggcg gccatagtcg
     2941 tcgagagtgc gaacgctgca atgcgcatgc gtcggacgtc gataccagcg cgccgtgcag
     3001 cctcagcgtt gccgccaacg gcgtaaaggt agcggccgaa ccgagtctta gtgagcagca
     3061 cggtcaaaat gacgagcagc accatgatca ggggaatgac ccacggcatg ccgataaggg
     3121 ccttacccaa cttgttgacc tgccggttgc ggttgagcag gtagacggcg aatacaccga
     3181 cgatcacgag tccggcgatc cgaaccagca ccaaggcgag tggttctgct gagagcttct
     3241 gagctctgcg ccgaatcgac cggaacaaag tgaatgccgc atagccacca acgaacacaa
     3301 cgaggaatat ccaaccgagc gtcgggctcg cattgctgtg cacaagctcg ttgatgggac
     3361 cgttgccgat gctgatcgcg ccggccttac ccgcgaacga gatgatgacg ccctgccagg
     3421 cgaggaagaa cgccagagtc acgatgaacg aaggaatgcc gactcgggcc accaggaagc
     3481 cggtgatgca accgatcgtg gtccccaccg cgagggctag gaacatcgcg atccagacgt
     3541 gtccaccgac gtgcgtcaag atgacgacga ggcccaacac gatcatcggc accgcgagcc
     3601 agagtcggtt gagcaccgcg atcaagagcc cgatcaccat gacgatgacg atcgcgccgt
     3661 agctgccgcc cttgaggtaa ccctcgatgt ttccgttgtg ggcgtacgca agccccatca
     3721 tcatggcgca gacgcccgcg gcggtacctg cggacaggtc gatctcgccg agcagcagca
     3781 caaagacgag acccatggcg atcaaaatcg tgtaaccggc ctggccggtg aagttcgcca
     3841 ggttaccgac ggtcaagaat tgattgctca ggctggagaa agcgacgagc aagataacga
     3901 ggccgagcaa cgccggcagc gctcccgggt cgccgccgcg caccttgctg acgtacgcgc
     3961 gtgcggaatc catcaaagtg agggagccca ggtcgccgct gaatgcctcc gcgactgcgt
     4021 cgtcggagat gggggtggtg atgacattcg tgatcggcag gtcagctgcg ctcgggccgg
     4081 cttcgcttcc cggggcccca gtgctttgtt cgctcatcgc ggcctcacgc gctttctgcg
     4141 gcagttgcgg gctggagccc gatggatccg gtgcgaccgg cggtgatgag ctcaaccact
     4201 tgtgagtacg tcacttcgac gcgcttgacc tctgcggcta cacgcccgag gaacagcgcc
     4261 gtgatgcggt cagcgacctc catcacgtcg ttcatgttgt gcgagatgag tacgacgccc
     4321 agaccactgt cggccaggcg ccgaacgagg tcgagcacct ggcgggtctg cgcgacaccg
     4381 agcgccgcgg tgggctcgtc gagcagcacg accttgttgt tccacagcac tgccttcgcg
     4441 atcgcgacgg actggcgctg gccgcccgac aggctcgaaa cctgctggcg aaccgatttg
     4501 accgtgcgca cggacaagcc agccagagtg tcacggctgc gg
//
LOCUS       DASWVF010000177         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_4875, whole genome shotgun sequence.
ACCESSION   DASWVF010000177 DASWVF010000000
VERSION     DASWVF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4875"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGD55_03065"
     CDS             complement(<1..324)
                     /locus_tag="VGD55_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869355.1"
                     /translation="MDDRLTLAGSAFGSASLVWVLYDQVLPFAGTLGFLICWYIAFLA
                     FYVALTAMSHPGPMVLDRLAQAVITGAAVVVGLALVFTIVYTFVRGWRPLVHLNFYTK
                     DMGGVQ"
     gene            complement(432..1445)
                     /gene="pstC"
                     /locus_tag="VGD55_03070"
     CDS             complement(432..1445)
                     /gene="pstC"
                     /locus_tag="VGD55_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0869356.1"
                     /translation="MTASALPMQRQDADGPRRIQTRPHPTDRVFRGVSRGIGALVLVL
                     TGAIGLFLAVQLFPVVHHYGFKFFTTQQWNVETNKVGVAAAIFGSVEVALVALLMAFP
                     LALGTALFITEYAPRRLKPLFVSMVDLMAAVPSIVYGLWGFYILQGLGLPVARWFAEW
                     FSFIPLLKVDADPHSAPFLKPQFTESPFLAGLVVAMMVIPLACAVMRGVFDQAPAGER
                     EAALALGSTRWGVIQSVVLPFGRGGIIGGTMLGLGRALGETVAVLIMLSQIDKINPHV
                     LQNGGVTVSSLIADHFGDANRGVQLEALLAAGFVLFVMTLLVNTIAGIIVTRSRSGAT
                     TEI"
     gene            complement(1442..2281)
                     /locus_tag="VGD55_03075"
     CDS             complement(1442..2281)
                     /locus_tag="VGD55_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY0869357.1"
                     /translation="MTDDMTAVLYATGEYPQLVFDHGAHLDAVNISAWFGSNKVLERV
                     NLSMPPSVVTALIGPSGCGKSTFLRILNRMHERVPSAKLNGEVRLNGADIYAAEERIT
                     DTRRRIGMVFQKPNPFPTMSIAENVVAGLKLNGTKVSRDERADLIERSLLRAGLWTEV
                     KDRLDTAGGALSGGQQQRLCIARSLAVSPDVLLMDEPCSALDPASTRRIEQTILDLKD
                     EVTIVIVTHNMQQAARVSQQCAFFLAETGTPGGIVEAGATDQIFGSPRDPRTSDYVQG
                     RFG"
     gene            complement(2278..3543)
                     /locus_tag="VGD55_03080"
     CDS             complement(2278..3543)
                     /locus_tag="VGD55_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0869358.1"
                     /translation="MGVFLAAAPWAFVLFAPMSEGASSPALAAQASASPSPPDYPPLL
                     VVPLDPAAVNNGGGAPGSSSGVVVSSAGPASPPPQWLVNSQLRQAASAVGPAPLVAPA
                     VLDSLKATGIPRRVLASYISAAATEDRLSPTCGLSWQVLAGIGFIESGHAHSGGSHNP
                     NWSGIADPPIYGPLLDGQNGIALIPDSDQGVLDGNATYDRAVGPMQFLPSTWREYEVG
                     ASGHSAPNPQNIDDATLSAARYLCATGPDLRTPDGLIAAVYSYNHSFDYVTAVLSVAI
                     RYAGGNLPGGASALAELPALAQTAADLPAAVAPPVAAVPSPTPSSLPYPSPLPTPPSS
                     PLPSQYPTYAPPPVVATSAPASPLSSSPPISAPASATPTADPTPTASPDPSPSPTGTA
                     PASSAPDSSAPASASPSPSASPAPTSETP"
     gene            3978..4472
                     /locus_tag="VGD55_03085"
     CDS             3978..4472
                     /locus_tag="VGD55_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869359.1"
                     /translation="MSTAVVVASVAGGSVTERRFVMVSTPVGSRVPWPNEPGEWFADD
                     QAPAATHSWSAWRLLGANNRELARSHGTYPDVASCAAAIRHLQSATNQLVAAFRPGFQ
                     TGRWYWQASVDGVPLAISARPFGWRRDCEHNFDQFVAALSGGVIEVGRLTVNFCDDQV
                     ATTG"
     gene            complement(4451..4903)
                     /locus_tag="VGD55_03090"
     CDS             complement(4451..4903)
                     /locus_tag="VGD55_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869360.1"
                     /translation="MRWFLARFCSALVAAFCVAAIPTHTSTVPEHPVPPVATAPAGNV
                     TLSVPTASIAPADGLPSVLARGHHYQLTVHVWVAGREPRTVATVSLAGAVRSACVVKA
                     VPAGTVATLRCTVTPTQIGAAGLRVSVSVVGAHGVPIVATFDHPVVAT"
     gene            5166..5588
                     /locus_tag="VGD55_03095"
     CDS             5166..5588
                     /locus_tag="VGD55_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869361.1"
                     /translation="MTQVRFMLVSAPAGSMVRWPGGWFGQTQTPMPHSSMWCAWRLLS
                     ANNRELGRSYTTFPDPPACAEAVALLRTRIDDAEASIVAGLRTGLWHWEVSVNAIPIA
                     ISPRSFTRQRECEHNLEQFLAGVNVVRKDVGAITESVS"
     gene            complement(5610..>6415)
                     /locus_tag="VGD55_03100"
     CDS             complement(5610..>6415)
                     /locus_tag="VGD55_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0869362.1"
                     /translation="TLAALGVVMMLFYGLRRECLFVTYWGIAWTTVAVYVVIPALDPA
                     HVYPYWDHGGHLSALLTSGLWTKLPTLAFVLLPTVFAALWSPIALVAVPGLLLRFVSG
                     ESLFWGTAGHYNATLMPIVFIAAIDGIARARSVPETARSHPVAALIGRHGAAMAIAAS
                     AAIAFQSPLSQLWNPSTYHVDARAHAADAAMKLIPSGTTVEASLNELAPLAARDDVYW
                     WSNVAPQWILFDSTSPEWQVSSDFIASRHAGVSYRTVFNRDGIWVYERT"
BASE COUNT         1193 a         2041 c         2155 g         1026 t
ORIGIN      
        1 ctgaacgccg cccatgtcct tcgtgtagaa gttcaggtgc acgagtggtc gccaaccgcg
       61 gacaaacgtg tagacgatgg tgaagacaag cgccaagcct acgacgacgg ctgcaccggt
      121 gatcaccgcc tgggcgagtc gatcgagcac catcggcccc ggatgtgaca tcgcggtaag
      181 ggcgacgtag aacgcgagaa acgcgatgta ccagcaaatt aagaagccga gcgttcctgc
      241 gaagggcagc acctggtcgt acaacaccca caccaacgac gccgagccga atgccgagcc
      301 cgccagcgtc agccggtcgt ccatggtcga tcggctcagc ttgcgaggct ggtcagggcc
      361 tgcaatccgc ccaccaccta cagggctcgg ccgcggtgcc gagtggcgtg caggtgcggc
      421 ggtcgtcatc gtcagatttc cgtcgtcgcg ccgctgcggc ttcgtgtgac gatgatgccg
      481 gcgatggtgt tgacgaggag tgtcataacg aacagcacga atcctgcggc gagcaatgcc
      541 tctagctgca cgccgcggtt ggcgtcaccg aagtggtcgg cgatgagtga cgagacggtc
      601 acgccaccgt tttgcaacac gtgcggatta atcttgtcga tctgcgagag catgatcagt
      661 accgcaaccg tctcgccaag cgcacggcca aggcccagca tcgtgccgcc gatgatcccg
      721 ccgcggccga acggcagcac caccgactgg atcactcccc agcgggtact gccgagtgcg
      781 agcgccgctt cgcgttcacc cgctggcgcc tgatcgaaga ccccacgcat caccgcgcaa
      841 gcgagcggga tcaccatcat cgcgacgacc aaaccggcaa ggaatggcga ctcggtgaac
      901 tggggcttca ggaagggagc gctgtgcggg tcggcgtcga ccttgaggag cgggatgaag
      961 ctgaaccact cggcgaacca gcgggcgacg ggcagaccta acccctgcaa gatgtagaaa
     1021 ccccacaagc cgtagacgat gctgggcacg gcagccataa ggtcgaccat cgagacgaac
     1081 aacggcttga ggcgccgcgg ggcgtactcg gtgatgaaga gcgcggtgcc cagcgccagc
     1141 ggaaatgcca tcaagagcgc aacgagggcc acctcgacgc tgccaaagat tgcggctgcg
     1201 acgccgacct tgttggtctc gacgttccac tgctgggtgg tgaagaactt gaagccgtag
     1261 tggtgcacga cggggaagag ctgcacggcg aggaagagcc cgatcgcgcc ggtgagcacc
     1321 agtaccagtg cgccaatacc acgcgacacc ccgcgaaaga ccctgtccgt cgggtgcggc
     1381 cgagtttgga ttcggcgtgg cccgtcggcg tcctgccgtt gcatcggaag tgcggatgcg
     1441 gtcatccgaa tcggccctga acatagtcgg aggttcgtgg atcgcgcggg ctaccgaaga
     1501 tctggtcggt cgcgcccgcc tcgacgatgc cgccaggcgt accggtctca gcgaggaaga
     1561 acgcgcactg ctgcgagact cgggccgcct gctgcatgtt gtgcgtgacg atgacgatcg
     1621 tgacctcgtc ctttaaatcg aggatcgtct gttcgatgcg acgggtcgat gcagggtcca
     1681 gcgccgagca cggctcgtcc atcagcaata cgtcgggcga gactgccagc gagcgcgcaa
     1741 tgcaaagtcg ctgctgctgc ccacccgaca gtgcaccgcc ggccgtgtcg agtcggtcct
     1801 tgacctcggt ccaaagccca gctcgaagca acgagcgctc gatcaggtcg gcgcgttcgt
     1861 cacgcgacac tttggttccg ttcagcttca gacccgcgac aacgttctct gcgatcgaca
     1921 tggtcgggaa cgggttgggc ttctggaaca ccatgccgat ccgtcgccgg gtgtcggtga
     1981 tgcgctcctc ggcggcatag atgtcagcgc cgttcagccg cacctcgccg ttgagcttcg
     2041 cgctcggcac ccgctcatgc atccggttga ggatgcgcag gaacgtcgat ttcccgcacc
     2101 ccgagggccc gatgagtgcg gtcacgacac tcggcggcat cgacaggttg acccgctcaa
     2161 gcaccttgtt cgacccgaac cacgccgaga tgttcaccgc gtcgaggtgc gcaccgtggt
     2221 cgaagactag ttgcgggtac tcgccagtgg cgtagagaac cgccgtcatg tcgtccgtca
     2281 cggcgtctcc gaggtgggtg cgggactagc gcttgggctc gggctcgcgc tcgccggagc
     2341 gctcgagtca ggtgcggacg acgccggtgc agtgcccgtc gggctcgggc tcgggtcagg
     2401 gctcgcagtc ggagtcgggt cagccgtggg cgttgctgat gctggtgccg atatgggcgg
     2461 ggacgacgac agcggggacg ccggagcaga tgtagcgacc accggtggcg gcgcgtatgt
     2521 cgggtattga gacggcagcg gcgatgaagg tggggtcggc aacgggctcg gatacggcag
     2581 cgacgaagga gtcggcgatg gaaccgcagc caccggaggc gccactgctg ccgggaggtc
     2641 ggccgcggtt tgtgccagcg ccggcagttc ggccagtgca gacgctccgc cgggcaggtt
     2701 tccgcccgcg tagcgaatgg cgaccgacag gactgccgtc acatagtcga aggagtggtt
     2761 gtagctgtag accgccgcga tcaacccgtc gggcgtgcgc aaatctgggc cggtagcgca
     2821 cagatagcga gcggccgaca gcgtcgcgtc gtcgatgttc tgcgggttcg gcgccgagtg
     2881 ccccgacgcc ccgacctcgt actcgcgcca ggttgacggc aggaactgca tgggacccac
     2941 cgcgcggtcg taggtagcgt ttccgtcgag cacgccttga tcggagtcgg gaatcaacgc
     3001 gatgccgttc tgcccgtcaa gcaggggacc gtagatcggc gggtcggcaa tgcccgacca
     3061 gttcgggttg tgtgatcccc ctgagtgggc gtggcccgac tcgatgaacc cgatacccgc
     3121 cagcacctgc cagctcaaac cgcacgtcgg agagagtcga tcttccgttg ccgccgcgct
     3181 gatgtaagac gcgagcaccc gcctcggaat ccctgtcgcc ttgagcgagt ccaacaccgc
     3241 cggagcgacc aacggtgcag gtccgaccgc ggaggcggcc tgccgcaatt gcgagttcac
     3301 caaccactga ggcggcggcg acgcgggtcc ggccgacgag accaccaccc ctgagctgga
     3361 tcccggggca cccccgccgt tgttcactgc ggccgggtcc agcgggacga cgagaagtgg
     3421 tggatagtcg ggcgggctcg ggctcgcact ggcttgggcg gcgagagcgg gcgaggatgc
     3481 gccctcgctc atcggcgcga acagcacgaa ggcccacggc gccgctgcaa gaaacacgcc
     3541 cattgcggcg acgagcggtc gcgtcccgcc cgtaggctgg acccgccgct ggtgcctggc
     3601 agatcgagtg cgtgcacctg ccggcacgcg ggacgtcgaa gcgtgcttgc gggtggactt
     3661 gcgtccagct acagagcggt ggcgtcccgg cgtttcgttc gcaaagacgt cgggcagatg
     3721 cgacacaatc cgcaccgtgc ccttcttgtc tcgtcgccgg cacccagaga ggcgacggct
     3781 gccagcgaac tgcatcggca cgcgccacag gctgtcgagg attagctaac gacggggtac
     3841 cgctgccgta agggcggatt tcgcggaggt gaacaagaaa ccttggcgag cgaaattcgc
     3901 gtcgttcacc ctccgcctat acggcgttca ggttctcgtg gtccttcgtt actgcaacca
     3961 acccagagtt cgagtacgtg tcgaccgctg tggtcgttgc gtctgtggcc gggggctcgg
     4021 tgaccgaacg tcggttcgtg atggtgtcga cacccgtggg gtcacgagtg ccgtggccca
     4081 acgagccggg cgagtggttc gccgacgacc aagctcctgc ggccactcac tcgtggtcgg
     4141 cctggcgact gctcggcgcg aacaaccgag aacttgcgcg cagccacgga acctaccccg
     4201 acgtcgcgtc gtgtgccgct gcgatcaggc acttacagag cgcaacgaac cagctcgttg
     4261 cagccttccg ccctggattt cagaccgggc ggtggtactg gcaagcatca gtcgacggcg
     4321 tcccgcttgc gatttccgcc cgcccgtttg gctggcggcg cgactgcgaa cacaacttcg
     4381 accagttcgt ggcggcgctc tcgggcggtg tcatcgaagt cggtcggctg accgtcaact
     4441 tctgcgacga tcaggtcgca acgaccgggt gatcgaaggt cgcgacgatc ggaacaccgt
     4501 gcgcaccgac cacagacacg cttacgcgca accccgccgc tccgatctga gtcggcgtga
     4561 ccgtgcaacg cagcgtcgcg accgttccgg ccggcaccgc cttcacgacg cacgcagacc
     4621 gaacggcgcc ggcaagtgag actgtcgcga cagtcctagg ttcacgacca gcgacccaga
     4681 cgtggaccgt gagttggtaa tgatgcccac gcgccagcac tgacggaagg ccatcagccg
     4741 gcgcgatcga cgcggtggga acgctcagcg tcacattgcc ggctggcgcc gtggcgactg
     4801 gcggaaccgg gtgttcgggg acggtgctgg tatgcgtcgg aatcgcagcc acacagaacg
     4861 cggccacaag agcactacag aaccgggcaa ggaaccagcg cacaatcgca gctctctgta
     4921 cgccgagttc ggtgcacccg gctgtttcga cggccgcttg tcatatcggt cgccaaacag
     4981 cacaagctga gcgaaatgtc acgacccacg cctgtgcatg acacaggccg ttcacccgat
     5041 tagtgtccgc cgttcactct ctcgtcggcc gtggttgccg caggtccagc acgattcggc
     5101 gcggctgcca tgtttcgcga ggacgttcct ccgcgaacct gcagcgccga cggcaagagg
     5161 aaaccttgac ccaagttcgc ttcatgttgg tgtcggcgcc cgccgggtcg atggtgcgct
     5221 ggcccggtgg gtggtttggg caaacccaga caccgatgcc gcatagctcg atgtggtgtg
     5281 catggcgatt gctgagcgcc aacaatcgcg agctcggtcg tagctacacg acattccccg
     5341 acccacctgc gtgcgctgaa gcagtggcgc ttctaaggac gcgcatcgac gacgccgagg
     5401 catcgatcgt tgccggtctg cggacaggcc tttggcattg ggaagtcagc gtgaacgcga
     5461 tcccaatcgc catctcaccg cggtctttca cccgccaacg cgagtgcgag cacaacctcg
     5521 agcaattcct cgcgggcgtc aatgtggtgc gaaaggacgt cggcgcaata accgaatcgg
     5581 tcagctgacg gcgtccggtc agagttgccc taggtgcgct cgtaaaccca aatgccatcg
     5641 cggttgaaca ccgtgcggta gctgaccccc gcgtgacggc tggcgatgaa gtccgatgag
     5701 acctgccatt cagggctggt ggagtcgaaa aggatccact gcggcgcgac gttgctccac
     5761 cagtacacgt cgtcgcgggc tgccagcggg gccaactcgt tgagactcgc ctcaaccgtc
     5821 gtgcctgacg ggatgagttt catcgccgcg tcagcggcat gcgcgcgagc gtcgacgtgg
     5881 taggtggacg ggttccaaag ctgtgacagc ggcgactgga aggcgatcgc agccgaggcc
     5941 gcgatggcca tggccgctcc gtgccgcccg atcaacgccg ccaccggatg cgagcgcgcc
     6001 gtctcaggca ccgagcgtgc gcgtgcgatc ccatcgatcg cggcgatgaa gacgatgggc
     6061 atgagagtgg cgttgtagtg ccccgcggta ccccagaaga ggctctctcc cgagacgaaa
     6121 cgcagcagca gtcccggcac cgccacgagc gcgatcgggg accacagggc tgcgaaaaca
     6181 gttggcagca gcacgaacgc gagcgtcggg agcttggtcc acagcccgga cgtgagcagc
     6241 gccgagagat gaccgccgtg atcccagtac ggatagacgt gcgccgggtc gagcgcagga
     6301 atgacgacgt agaccgcgac tgtcgtccac gcgatgcccc agtaggtgac gaacagacac
     6361 tcgcgccgca gaccgtagaa cagcatcatg acgacgccga gtgcggcgag ggtga
//
LOCUS       DASWVF010000178         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_259839, whole genome shotgun sequence.
ACCESSION   DASWVF010000178 DASWVF010000000
VERSION     DASWVF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259839"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VGD55_03105"
     CDS             <1..347
                     /locus_tag="VGD55_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0869363.1"
                     /translation="CDQTKGLAAMQDILTAHPDVTAVYGACGPPTTGAVQAIKAKGKT
                     GIVVVGFDADAPQITDILAGNELASVIQFPAKMGSMGIDTLVKVIEGQTVPANIDTGT
                     GLVTKDNASQFQ"
     gene            444..1772
                     /locus_tag="VGD55_03110"
     CDS             444..1772
                     /locus_tag="VGD55_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0869364.1"
                     /translation="MPVIHAVDVIDVRFPTSLELDGSDAMHTDADYSAAYVVLRTNDP
                     ELSGFGLTFTIGRGNEVCVAVARQLAVVLIGMDVDVMLTDMGAVYRALTADSQLRWLG
                     PEKGVVHLSLAAVLNAAWDLVARRAGKPLWRVLVDMPARDLAAVADLRYVTDVLTRDE
                     AESMLAALEPTREQRIAELERVGYPCYTTSPGWLGYDDEKLARLCQEAVEAGFRHIKL
                     KVGANLEDDRRRCAIARRALGTDGDLMLDANQVWDVRQAIAWVNALADFKPLWIEEPT
                     SPDDILGHAAIRAAVAPIRVATGEHGHNRVMFKQFLQAGSIDYCQIDAGRLASVNEVI
                     TVLLLAAKFGVPVCPHAGGVGLCEMVQHLAIFDYVAVSGRLDGRVAEYVDHLHDHFVE
                     PCIVKDAAYVVPTAPGYSAQMHAASVAQYRYPDGMYWAAYAAGRTPSIDR"
     gene            complement(1855..2154)
                     /locus_tag="VGD55_03115"
     CDS             complement(1855..2154)
                     /locus_tag="VGD55_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEY0869365.1"
                     /translation="MVGTFVTFNYGNDFDRARVESVAEHARQNFGGMPGLHSKVFTVD
                     ENNRRAINVYVWDSAEAAHAFFSEQMVERVTGLYGVRPVVDIVDIAAIVENAISV"
     gene            2361..>3039
                     /locus_tag="VGD55_03120"
     CDS             2361..>3039
                     /locus_tag="VGD55_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0869366.1"
                     /translation="MYREKLELEGRVAVVTGGARGIGHAIVEALREMGARCVVLGLDD
                     EQGSAAVAALSASGAAVAYRHLDVRDQEAVATEFEQIAQTYGAIDILVTCAGVVSHKP
                     TADVTEADWRFVLDTNLDGTFWCARAAVKQMQRKNTRGAIVTIGSMSGLIVNFPQMQA
                     SYNASKAAVHSLTASLAAEYAESGIRINSVAPGYIMTEMTKGGVSAEWLDLWTSHTPM
                     KRLGKPDE"
BASE COUNT          548 a          927 c          998 g          566 t
ORIGIN      
        1 attgcgacca gaccaagggc ttggctgcga tgcaagacat cttgaccgca caccccgacg
       61 tcactgctgt gtatggagcc tgcggaccgc cgaccacagg ggcagtgcag gcgatcaagg
      121 cgaagggtaa gaccgggatt gtcgtcgtcg gtttcgacgc tgacgcgcct cagatcaccg
      181 acatcctcgc gggcaacgaa ctcgcgtcgg tcattcagtt cccggcgaag atgggatcga
      241 tgggcatcga cactctcgtc aaggtgatcg agggccagac agttccggcc aacatcgaca
      301 ccggcaccgg cttggtgacc aaggacaacg cgtcccaatt ccagtagcag tcatggtcgc
      361 gccctgccgc tggtgatgcc agcggcaggg cgtttaccgg ccggatcccc ggccggttcc
      421 gcctcaccac caggggttgc ccagtgcccg tcatccacgc ggtggatgtc atcgacgtcc
      481 gcttcccgac gtccctggaa ctcgacggtt ccgatgcgat gcacacggac gccgactact
      541 cggcggctta cgtcgtgttg cggaccaatg atccggagct ttcggggttt ggtttgacgt
      601 tcacgatcgg gcgcggcaac gaagtgtgcg ttgctgtcgc gcggcagctg gccgtcgttc
      661 ttatcggcat ggacgtcgat gtgatgctca ccgatatggg tgccgtgtat cgagcgctca
      721 ctgccgattc gcagttgcga tggctggggc cggagaaagg tgtcgtccac ctgtcgcttg
      781 cggctgtgtt gaacgccgcc tgggatctcg tcgcccgtcg tgcaggcaaa ccgttgtggc
      841 gtgtgttggt cgacatgccc gcccgcgacc tagccgcggt cgccgacctg cgttacgtga
      901 ccgacgtcct gacccgtgac gaggccgaaa gcatgctcgc cgctctcgag cccactcgcg
      961 agcagcggat tgccgagctc gagcgcgttg ggtatccgtg ctacaccacg tcgccggggt
     1021 ggttgggcta tgacgacgag aagctcgccc gcctgtgcca ggaagcggtc gaggcgggct
     1081 ttcgccacat caaactgaag gtgggcgcga atctcgagga cgaccgccgg cggtgtgcga
     1141 tcgcgaggcg agccctcggt accgacggtg acctgatgct cgacgcgaac caggtctggg
     1201 atgttcggca agcgatcgcc tgggtgaacg cactcgcgga cttcaagccg ttgtggatag
     1261 aggaaccaac gagtcctgac gacatcttgg gccacgccgc gattcgagca gccgtcgcac
     1321 cgatccgggt cgccactggc gagcacggtc acaaccgcgt catgttcaag cagttcttgc
     1381 aggccggatc gatcgactac tgccagatcg acgccggtcg gctggccagt gtcaacgagg
     1441 tcatcactgt gctcctgctc gcggcgaagt ttggcgtgcc cgtgtgcccg cacgcgggag
     1501 gtgtcgggct gtgcgaaatg gttcagcacc tggcgatttt cgactacgtc gcggtgtcgg
     1561 gaagactcga cgggcgggtc gctgaatacg tcgatcacct gcacgaccac ttcgtcgaac
     1621 cgtgcattgt gaaggacgcc gcgtatgtgg tcccgaccgc gccgggctac agtgcgcaga
     1681 tgcatgctgc gtcggtcgcg caataccgct accccgacgg aatgtactgg gccgcgtatg
     1741 cagccgggcg tactccgtcc atcgatcggt agcgcgactc gttatcgctg ggaccgcaga
     1801 cttcccgcac cgcgcagccg gcggaccttt agcggcttcc tatttagcgg cggatcagac
     1861 gctgatggcg ttctcgacaa tcgcggcgat gtcaacgatg tcgaccacgg gtcggacgcc
     1921 gtaaagcccg gtgaccctct ctaccatttg ctcggaaaag aaggcatgag ctgcctcggc
     1981 ggaatcccac acgtagacgt ttatggcccg gcgattgttc tcatcgaccg tgaacacctt
     2041 gctgtgcaaa cccggcatgc cgccgaagtt ctgacgcgca tgctcggcaa ctgactcgac
     2101 gcgagccctg tcgaagtcat tgccgtaatt gaaggtgaca aatgttccga ccatgggtcc
     2161 tcctcgattc ggtggatggc gcggtcgatg tggatcgggc agtcgccggg caccgagcag
     2221 cgaccgttcg atcatgcccg ttcttgcgaa atcccgtcaa aggaccggcg atccttgccc
     2281 gaccactcgg gagcttgctc accggactct gaacgtcgac gctggtacac gcacaggacc
     2341 cttcagctaa aggagttcgt atgtaccggg aaaagctcga gcttgaaggg cgggtcgcgg
     2401 ttgtcaccgg tggggcccgg gggatcggcc acgcgatcgt cgaggcgctg cgggagatgg
     2461 gtgctcggtg tgtggtcctt ggactcgacg acgagcaggg gagcgcagcc gtggcggcgc
     2521 tgagcgcgag cggagcggcc gttgcgtacc gccacctcga cgtcagggac caggaggcgg
     2581 tcgcgactga gttcgagcag attgctcaga cctacggtgc gatcgacatc ctggtgactt
     2641 gcgccggcgt cgtgtcgcac aagccaacag ccgacgtgac cgaggcagac tggcgatttg
     2701 ttctcgatac caatctcgac ggcacgtttt ggtgtgctcg cgcagcggtc aagcaaatgc
     2761 agcggaagaa cacccgaggt gcgatcgtga ccatcggttc gatgtcaggg ctcatcgtca
     2821 acttcccgca gatgcaggct tcgtacaacg cgtccaaagc agccgtgcac tcacttacgg
     2881 cgagcctggc cgccgagtac gcggagtcgg gcatccgcat caactcggtc gcgccgggct
     2941 acatcatgac ggagatgacg aagggtggcg tctcggcgga gtggcttgat ctgtggacgt
     3001 ctcacacacc gatgaagcga ctgggcaagc ccgacgagg
//
LOCUS       DASWVF010000179         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_463593, whole genome shotgun sequence.
ACCESSION   DASWVF010000179 DASWVF010000000
VERSION     DASWVF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463593"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGD55_03125"
     CDS             <1..198
                     /locus_tag="VGD55_03125"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEY0869367.1"
                     /translation="ELVRGVRYRGGGAVTSSLVMRSKSGTIRTIESEHQLSKLRGYAI
                     VDYDHRHADHTLASPDRTRLP"
     gene            300..1712
                     /locus_tag="VGD55_03130"
     CDS             300..1712
                     /locus_tag="VGD55_03130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0869368.1"
                     /translation="MTPETSETGFRVEHDSMGDVRVPGHAKWRAQTQRAVENFPVSGL
                     AIDRDLIGALASIKGAAALVNAELGVISPSIAQAIYAAAVDVARGDWDPHFPIDVFQT
                     GSGTSSNMNANEVLATLATERLGAPVHPNDDVNASQSSNDTFPAAIHIAATRSIVVNL
                     IPALAHLEASLARKAQEFSTVVKSGRTHLMDATPVTLGQEFGGYAASMRLGVERLQAA
                     LPRIGELPLGGTAVGTGLNAPAGFAAKVIASLSSSMQLPLTEARDHFEAQGSRDALVE
                     ASGVLRVVAVSLYKMANDLRLMSSGPRTGFAEIHLPDLQPGSSIMPGKVNPVLPEAVC
                     QVVAQVIGNDAAIAFGGAAGNLELNVMLPVIARNLLESIRLLSNISRLLADRCIDGIE
                     ADVDRCRMFAESSPSIVTSLNKYIGYEAAAKVAKLSLAQNKTIREVVLESGYVEAGKL
                     TEAQLDEALDVLAMTRPPKS"
     gene            complement(1776..>2138)
                     /locus_tag="VGD55_03135"
     CDS             complement(1776..>2138)
                     /locus_tag="VGD55_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0869369.1"
                     /translation="PIGVVAQVVAKAVVVALPAIAGTPRQMAYAVAVRAGLSAQQWSC
                     LSSLWQQESKFQTTARNGRSGAYGIPQALPASRMASAGADWRTNPVTQIRWGLSYIST
                     RYGTACTAWSHWKRDRWY"
BASE COUNT          377 a          709 c          690 g          362 t
ORIGIN      
        1 gaacttgtgc gcggtgtgcg atacagaggc gggggggctg tcaccagctc gctggtcatg
       61 cgctcaaaga gcggcacgat ccgcaccatc gagagcgagc accagctgtc gaagctgcgc
      121 ggctacgcca tcgtcgacta cgaccaccgg cacgccgatc acaccctcgc ctcgcccgac
      181 cgcactcggc tcccctgaga ttcaagctga gattcgaact gagagcgcct cgcccgacgt
      241 gagcaagacc acgctgtcct gagcggtccg gtcacgggct ggagttctac gctgcactca
      301 tgacccccga gacctccgaa accggcttcc gcgtcgagca cgacagcatg ggggacgtcc
      361 gcgtcccagg ccatgccaag tggcgcgcgc agacacagcg tgcggtcgag aatttcccgg
      421 tctcaggctt agcgatcgac cgcgacttga tcggtgcact cgcgtccatc aagggcgcgg
      481 ccgcattggt gaacgccgag ctcggggtga tcagcccgtc gattgcgcag gcgatttacg
      541 ccgccgcggt cgacgtggcc aggggcgact gggacccgca ctttccgatc gacgtcttcc
      601 agaccgggtc gggtacttcg agcaacatga acgccaacga ggtgctggcg acgcttgcca
      661 cggagaggct cggagcaccc gttcatccca acgacgacgt caacgcgtct caatcgtcga
      721 acgacacgtt cccggccgcg atccacatcg cggccacccg ctcgatcgtg gtcaatctta
      781 tccctgcgct ggctcatctc gaggcgtcgt tggcacgcaa ggcacaggag ttctcgaccg
      841 tggtcaagag cggccgcaca cacctgatgg atgccacgcc agtgacgctc ggtcaggagt
      901 tcggcggcta cgcggcgtcc atgcggcttg gcgttgaaag gttgcaagcg gcactgccgc
      961 gcatcggcga gctcccgttg ggcgggaccg cggtcggcac cggcctgaac gcacctgctg
     1021 gtttcgctgc gaaggtcatc gcctcgttgt catcgtcgat gcagctgccg ctaaccgaag
     1081 cgcgcgacca tttcgaggcg cagggctcgc gtgacgcgct cgtcgaggcc agcggcgttt
     1141 tgcgggttgt cgcggtgagc ctctacaaga tggcgaacga cctgcggttg atgtcgtcgg
     1201 gcccgcgcac cggattcgca gagattcacc tgcccgacct tcaacccggc tcgtcgatca
     1261 tgcccggcaa ggtgaatccg gtattgcccg aagctgtgtg tcaggtcgtc gcacaggtca
     1321 tcggcaacga cgccgcgatc gctttcggcg gtgctgcagg caatctcgaa ctcaacgtga
     1381 tgctgcccgt catcgcgcgc aacctgctcg agtcgatccg gctgctctcg aacatttcca
     1441 ggctgctcgc cgatcggtgc atcgacggca tcgaggcgga cgtcgaccgg tgccgcatgt
     1501 tcgcggagtc atcgccgtcg atcgtcacgt cactgaacaa gtacatcgga tacgaggcag
     1561 ccgcgaaggt cgcgaaactg tcgctcgcgc agaacaagac gattcgtgag gtcgtcctcg
     1621 agagcggcta cgtcgaggcg ggcaagctca ccgaggccca actcgacgag gcgctcgacg
     1681 tgctagcgat gaccaggccg ccaaagtcgt agcggcgccc agtattgggg agctcagtat
     1741 tggaagctca gtaccatgcg ctcagcacca ggcgctcagt accagcggtc gcgcttccag
     1801 tgcgaccagg cggtgcacgc cgtgccgtaa cgggtgctga tgtagctgag gccccaccgg
     1861 atctgggtca ccgggttggt ccgccagtcg gcaccggcgc tcgccatgcg agacgcgggt
     1921 agcgcctggg ggatgccgta ggcgcccgac ctgccgttgc gcgccgtggt ctggaacttg
     1981 ctctcctgct gccacagcga tgacaggcat gaccattgct gcgcgctgag accggctcgc
     2041 accgcaacgg cgtaggccat ctgtcgaggg gtacccgcga tcgcgggtag cgcgaccacg
     2101 accgccttcg cgacgacctg tgccacaacc ccgatcgg
//
LOCUS       DASWVF010000180         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_30375, whole genome shotgun sequence.
ACCESSION   DASWVF010000180 DASWVF010000000
VERSION     DASWVF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30375"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGD55_03140"
     CDS             <1..389
                     /locus_tag="VGD55_03140"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0869370.1"
                     /translation="CVGEALEIRKAGQQVPHTLVQLDGGLADIAAEHVAQIVIAYEPV
                     WAIGTGEVATPEDAQEVCAAIRARLVELYEPSVADGARVLYGGSVKGINAAGIMAQRD
                     VDGALVGGASLDPDEFVRIVRFNNTV"
     gene            complement(557..838)
                     /locus_tag="VGD55_03145"
     CDS             complement(557..838)
                     /locus_tag="VGD55_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869371.1"
                     /translation="MRRLPVFVSPAALGEVAAALLAATGALALPFGAVVALVLAEAAG
                     VVLVAVLFLLLLQAASARATMATPAAICVPLRLTSMSLLLAAALWVVRE"
     gene            870..>1698
                     /locus_tag="VGD55_03150"
     CDS             870..>1698
                     /locus_tag="VGD55_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0869372.1"
                     /translation="MTKGGTLTYTLEKTIDNWNVLTSAGDTFETAEVENAIYPNAFNV
                     LPDLGPVLNTDLLTSAAVTTSSPQTIVYVINPKAVWSDGTPITADDFTYLWKAQDGVD
                     CTKCDIASSTGYAQIASITGSNSGQTVTVVMKTPFSDWKSLFSNLMPYHSASPQGLDQ
                     VNLEKSWDTGFATTPPAVSGGPFVVSDFQKDQSVTLSPNPKWYGANGPYLDKLVFRMI
                     TDATQEPTALQNGEVDAIYPQPEVDLISQLNGMSNIVYQLDLGLVFEHIDLNLQTPAL
                     "
BASE COUNT          348 a          531 c          529 g          290 t
ORIGIN      
        1 tgtgcgttgg cgaggcactg gagatacgca aggccgggca gcaggtgccg cacaccctgg
       61 tccagctcga cggcggcctc gccgatattg ctgcagaaca cgttgcgcag atcgtgatcg
      121 cttacgagcc ggtctgggca atcggcaccg gtgaggtcgc cacgcctgag gacgcccagg
      181 aagtgtgtgc ggccatccgg gcccggctcg tggagctgta tgagccgtcg gtcgccgatg
      241 gcgcgcgggt gttgtacggg ggctcggtca agggaattaa tgccgctggg attatggctc
      301 agcgtgatgt cgacggtgct ttggtgggag gtgcaagcct cgatccagac gagttcgtcc
      361 gtattgtccg atttaacaac acggtctagc gtcactttgg tgacagctcg tctaccgaga
      421 tccactgcgt aactcttgtg tcacgatttg cgttaggttc agcccaaaga atgctcgacg
      481 aagcatccgg tggctacagt caccgcgacc gctgcggggg gcgttcgcct accaaaagcc
      541 cggcgtacgg gagttactac tcgcggacca cccacaaggc ggccgccaga aggagagaca
      601 tgctcgtcag acgtagaggt acacagattg cggcaggagt cgccatcgtc gctcttgcgc
      661 tcgctgcctg cagcagtagc aagaagagca cagcgacgag cacaactccc gctgcttcgg
      721 ctagtacgag cgcgactaca gcgccgaacg gtagcgccag cgcaccggtt gcggccagca
      781 gcgcggcggc aacctccccg agtgccgcgg gcgagacgaa gacgggcaac cgcctcaccg
      841 gtaacccgga cacgatcaac actgcctcag tgaccaaggg cggcacgctc acctacacgc
      901 ttgagaagac aatcgacaac tggaacgtgt tgacgtccgc gggtgacacg ttcgagaccg
      961 ccgaggtcga gaacgcgatc tacccgaacg cgttcaacgt gctgcccgac ctgggtccgg
     1021 tgttgaacac cgacctcctc acgagcgcgg ccgtcacgac ctcgtcaccg cagacgatcg
     1081 tgtacgtcat caaccccaag gctgtgtggt ctgacggcac cccgatcacc gccgacgact
     1141 tcacgtacct ctggaaggcg caggatggtg tcgactgcac gaagtgcgac atcgcgtcga
     1201 gcacaggtta cgcgcagatc gcatcgatca ccgggtcgaa cagtggccag acggtcacgg
     1261 tggtcatgaa gacgccgttc tccgactgga agtcgctgtt cagcaacctg atgccgtacc
     1321 attcggccag cccgcagggt ctcgaccagg tcaacctgga gaagagctgg gacaccgggt
     1381 tcgctaccac accgccggcc gtttccggtg gtccgtttgt tgtgtcggac ttccagaagg
     1441 accagagcgt cacgctcagc ccgaacccga agtggtacgg agctaacggc ccgtacctcg
     1501 acaagctcgt gttccggatg atcaccgacg caacgcagga gccgacggcg ttgcagaacg
     1561 gtgaagtcga cgcgatttac ccgcagcccg aggttgacct catcagccag ctcaacggga
     1621 tgagcaacat cgtctaccag ctcgacctgg gtctggtatt cgagcacatc gacctcaacc
     1681 tgcagacccc ggcgttgc
//
LOCUS       DASWVF010000181         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_183321, whole genome shotgun sequence.
ACCESSION   DASWVF010000181 DASWVF010000000
VERSION     DASWVF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183321"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VGD55_03155"
     CDS             complement(<1..605)
                     /locus_tag="VGD55_03155"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0869373.1"
                     /translation="MSLAPDALRESATGRHRRPISLDVALKQSVKQSLRTPVREVREL
                     RDPVLWSVVGVVLLVSCVLSLSRYIRVSPATWDLGIFTEAIHRYAHLQSPTVDIKSPG
                     FDLLGDHFHPILVLLAPFFRVFPTPATLLVAQALLTALSVIPVYRATQQLLGQTEARL
                     IAAAYGLSWGLAAMNAYDFHEVCFAVPLLAASLSCLIRGNVR"
     gene            838..1317
                     /locus_tag="VGD55_03160"
     CDS             838..1317
                     /locus_tag="VGD55_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869374.1"
                     /translation="MAAVRLLFVVSGRAGVWSAPGLEPEQREINLDGWCAWRLVGANN
                     REIARSARTFPTFHECYQAARQVQQRLALAVTTVRHDDVLNLWAWRAQLDGLPVATSG
                     RLYQRHRECQSNVAQFLALMPLAATAYDSTVVPAAEGDSFEDDQRYDEVASTTLRLV"
     gene            complement(1331..1687)
                     /locus_tag="VGD55_03165"
     CDS             complement(1331..1687)
                     /locus_tag="VGD55_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869375.1"
                     /translation="MNGSLPPGLLPVGGVGGGVSKVTPNTVGVTDGLDDGLPPPAGHA
                     GLLAVVVANPCELSSGIVSSVGTPGGLESDASASDGVAAIPALNTSTATARPLGRSEC
                     VRMAGFSTHGPGSNGR"
     gene            complement(1807..1880)
                     /locus_tag="VGD55_03170"
     tRNA            complement(1807..1880)
                     /locus_tag="VGD55_03170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Thr,seq:tgt)
     gene            1925..3160
                     /locus_tag="VGD55_03175"
     CDS             1925..3160
                     /locus_tag="VGD55_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEY0869376.1"
                     /translation="MRLQFATTADPAELLALPWSTPLDQWPADQLVSLPRGISRHVVR
                     FVRVSGTVYAIKELPQQVAEREYRLLQELGKREVPVVRARGVVSDRATEDGEPLDSAL
                     VTKHLRYSLPYRALFSRRLDPELADKLLDALAQLLVRLHLVGFAWKDCSLSNTLFRRD
                     AGAFAAYLVDAETGELHATLSDGQRVQDLDIASVNIAGELFDLEAAGLLAENVEATDV
                     ADSVISRYEALWTELTRSEYVDPNEWWRIEHRLQRLNDLGYDVGEVQLIDEPGGHRLK
                     LQTQVVEAGHHQRRLSQLTGLNVEENQARRLLNDLDTFRAAAVMPGTDVDEETVARHW
                     LDDVFEPVIEQIPSELRGRLEPAEIFHEVLVHRWFLSEAAGHDVGMDAATRSYVDNVL
                     KFRPDERAILGVPAGPASG"
     gene            complement(3180..>3452)
                     /locus_tag="VGD55_03180"
     CDS             complement(3180..>3452)
                     /locus_tag="VGD55_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY0869377.1"
                     /translation="NLTRALTGYAERGLLVVGLAGGGSLDLMDLEAATEPIVLVVGSE
                     GKGLSRLVQERCDLVVRIPISSATESLNASVAAGVALYEVARRRKA"
BASE COUNT          664 a         1140 c         1089 g          559 t
ORIGIN      
        1 cggacgttgc cacggatgag gcacgacagc gacgccgcga gcagcgggac ggcgaagcac
       61 acctcgtgga agtcgtaggc gttcatcgcg gcgaggcccc aggaaaggcc gtaggccgcg
      121 gcgatgagcc gcgcttctgt ctgcccgaga agctgttgcg ttgcccggta caccgggatg
      181 accgaaagtg cggtcagcag cgcctgcgcg acgagcagcg tggccggggt cgggaagacg
      241 cggaaaaaag gagcgagcaa caccaggatc gggtggaagt ggtcgccgag caggtcgaag
      301 ccgggcgact tgatgtcgac ggtcggcgac tggaggtgcg cgtaacggtg gatcgcttcg
      361 gtgaagatac ccaggtccca ggtcgccggc gacaccctga tgtagcggga gagcgacagg
      421 acacacgaca cgagcagcac tactccaacg accgaccaca acaccggatc tcgcagctca
      481 cggacctcgc gaaccggcgt ccgcaaagac tgcttcaccg attgctttag cgccacatcg
      541 agtgagatcg gacgccgatg gcgaccggtt gccgactcgc gcagcgcgtc gggcgccagc
      601 gacatgacac tccctgcacg acgatgcgac gagctgctcg gaaacgccca agacgggtgc
      661 tgccttgcga gtttgcaaca caatcgaggc cggcaggcga aggacagatg aacggcggat
      721 agagattggc ttcggagctt gggaacgaga ggcggatctg acagcttgtg acgcagcggg
      781 gagcaggccc tcttggggag acgtgcgcgc tagccgcacg cagagaaggg aagcaacgtg
      841 gccgcagtcc ggctgctgtt tgtggtgtct ggtcgtgcgg gggtgtggtc ggctccgggt
      901 cttgagcccg agcaacgtga gatcaatctc gacggttggt gcgcatggcg actcgtcggc
      961 gcgaacaacc gcgaaattgc tcgaagtgca aggacattcc cgactttcca tgagtgttat
     1021 caagcggcgc gccaggtgca gcaacggttg gcacttgctg tcacgacggt caggcacgac
     1081 gacgtcctga atctgtgggc gtggcgagcg caactcgacg gtttgccggt cgcgacgagc
     1141 ggtcggctct atcaacggca tcgcgagtgc cagtcgaacg tcgcccagtt cttggccctc
     1201 atgccgctcg cggcgacggc ctacgactcg actgtcgtgc cggcagccga gggcgacagc
     1261 ttcgaagacg accagcgtta tgacgaagtc gcgagcacga cgctccgact cgtctgacgg
     1321 cctcgtagcg tcagcgacca ttactgccag ggccgtgcgt actgaacccg gccatgcgca
     1381 cgcattcgct cctgcccagg ggtcgagcgg tcgccgtgct cgtgttgagt gccgggatcg
     1441 ccgcgactcc gtcgctggca ctcgcgtccg actccaaccc accaggggtg cccacgctgc
     1501 tgacgatccc gcttgacagt tcgcaggggt ttgcgacgac aacagctagc aacccggcgt
     1561 gccctgcagg tgggggcagc ccgtcgtcga gcccatcggt gacgccaacc gtgttgggcg
     1621 tcacctttga caccccaccg ccgacgccgc caacgggcag caagcctggc ggcaaactgc
     1681 cgttcaccgg actcccgttg gttcagtcgg tcgtcgtcgg ttccgcgctc atcgcctcag
     1741 ggtcggggct ggtgtactcg aggcggcgcc acgagtcgtc taagagtcga agaaattcgc
     1801 gatgagagcc ggcgagggga attgaacccc taacctcccg attacaagtc gggtgcgctg
     1861 ccagattgcg ccacaccggc gggactgctc gccgatcgta cgccgcgcgt gtgagagtag
     1921 tgacgtgcgt ttgcagttcg ccaccaccgc cgaccccgct gaactcctgg cgctgccgtg
     1981 gtcgaccccc ctcgaccagt ggcctgccga ccagcttgtc tcgctgcctc gaggcatctc
     2041 gcgccacgtc gtccgcttcg ttcgcgtctc cggaaccgtg tacgcgatca aagagcttcc
     2101 ccagcaagtc gccgagcgcg agtaccgcct cctgcaggag ctcggcaagc gcgaggtccc
     2161 cgtcgtccgc gcgcgtgggg tcgtaagcga cagggctacc gaggacggcg agccgctcga
     2221 ctcggcgctc gtgaccaagc acctgcgtta ctcactgcca taccgcgcgc tgttctcgcg
     2281 acggctcgat cccgaactcg ccgacaagct gctcgatgca ctcgcccaac tactcgtgcg
     2341 cttgcacctc gtcggcttcg cgtggaagga ctgctcgcta tcgaacacgc tgttccgccg
     2401 tgacgccggc gcttttgccg cgtacctggt cgacgccgaa accggcgagc tacacgccac
     2461 gctttctgac ggccaacgtg tgcaggatct cgacatcgcg tccgtcaaca tcgccggcga
     2521 attgttcgac ctcgaagcgg caggattgct cgccgaaaac gtcgaagcga ccgacgtcgc
     2581 cgacagtgtt atttcgcgct acgaagcgct gtggaccgaa ctcacccggt ccgagtacgt
     2641 cgacccgaac gagtggtggc gcatcgaaca tcgcctacag cgcctaaacg atctcggcta
     2701 cgacgtcggc gaagtgcaac tcattgacga gcccggcggg catcgcctca aactccagac
     2761 acaagtcgtc gaggctggcc accaccaacg gcgcctttca caactcacgg gcttgaacgt
     2821 cgaagaaaac caagctcggc ggctgctcaa cgatctcgac acgttcaggg ccgcggccgt
     2881 catgcccggc accgatgtcg acgaggaaac ggtcgcccga cactggctcg acgacgtatt
     2941 cgagccggtc atcgaacaaa ttccgtccga acttcgtggc cgcctcgagc ccgcagaaat
     3001 cttccacgaa gtgctcgtgc atcgctggtt cctgtccgaa gccgctggcc acgacgtcgg
     3061 catggacgcc gcaacgcgct cgtacgtcga caacgtgctc aagttccggc ccgacgaacg
     3121 cgccatcctc ggcgttcccg caggcccggc gagcggctga gccctaccgc caacaaacgt
     3181 cacgccttgc gacgacgcgc cacttcatac aacgcaacac ccgcggcaac tgacgcattg
     3241 agcgactcgg ttgcagaaga aataggtatg cggacgacga ggtcgcaccg ttcttgcacc
     3301 agtcgagaca accctttgcc ttccgaaccg acaacaagca cgatcggctc ggtcgcggcc
     3361 tcgaggtcca tcagatccaa cgaccctcca cccgcaaggc cgacgacgag taacccgcgt
     3421 tcggcgtacc cagtcaaggc tcgagtcaag tt
//
LOCUS       DASWVF010000182         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_259900, whole genome shotgun sequence.
ACCESSION   DASWVF010000182 DASWVF010000000
VERSION     DASWVF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259900"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VGD55_03185"
     CDS             complement(<1..1085)
                     /locus_tag="VGD55_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869378.1"
                     /translation="MAAGLNGAAPPGLQRTLSPGGFLAIAVVSFGGPLALAALYVPGL
                     VDDVFSSAGLVTVIAPLAFAFPLLIWLRYAREISSSGGLFSFVEAAAGRRVALVQATI
                     WTVSYLLYLVYTTVYVVYDVLPTVIPGVQPYRSTLEVAIPIALAILVIAGRSPAVVVL
                     SIFAVGQLVLVGLLAVVTIAHAHPMSSFTTTSDVGGIASASGNVALLFVCGSLPFFFG
                     GEVAQPARAVRRGVVTAYVLVVVAVVAAVFPLAANPAFTHAEIPGASVVGVYVGHAAG
                     VAVGIGVAASIVGVMLVEYLALTRLLHAVRGTSIKTASRLVAIPLVIGGPLSLINPDR
                     FYSDLLKPSLIALWLSQIIVVAVFPLFL"
     gene            complement(1069..1779)
                     /locus_tag="VGD55_03190"
     CDS             complement(1069..1779)
                     /locus_tag="VGD55_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869379.1"
                     /translation="MNNVTRTTAPVIKATVFLGLAALLMAGCASKKSNTAATNSAPAT
                     TAAAAAAGTPAPSAAATSVAGVAPAAASGAAVGASAAPAAGPAAVDVKTGPLGTYLTD
                     ATGHTLYLYTPDTSSTSTCYGQCIAFWPPLLTSSAPQAGSGAEASQLGTTMRTDGTMQ
                     VTYKSHPLYFFKGDKSVGDTTGQGKEGTWFLVSPAGAQIGSAPAAKASAASKTNPAAA
                     APSPTASKSSAAAGGGWS"
     gene            1901..>2346
                     /locus_tag="VGD55_03195"
     CDS             1901..>2346
                     /locus_tag="VGD55_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869380.1"
                     /translation="MPKDSAAGSTLLGSLVGSLDAAPPYLLAEVLHNFLNAEVGARSS
                     PVLLADYEERMLEPVPGGAPSPTLGAQQIDGSQAGRAYREQRLVEIEASGEVTLYLPI
                     TQRAERVGVLEITMPTPGIDVRQQLTEVARILAYFIAAARRYTDQF"
BASE COUNT          445 a          762 c          771 g          368 t
ORIGIN      
        1 aggaagagcg ggaacaccgc gacaacgatg atctgcgaaa gccatagcgc gatcagcgac
       61 ggtttcagca ggtcactgta gaagcggtcg gggttgatca gactgagagg cccaccgatc
      121 acaagcggga tcgccacgag tcgcgaggcc gttttgatgc tggtgccgcg cacggcatgg
      181 agcagacgcg tcagagccag gtactccacg agcatcacgc cgacgatgct ggccgccacg
      241 cctattccaa ccgcgacacc tgccgcgtgc ccaacgtaga cgccgacgac cgacgcgccg
      301 ggaatttcgg catgagtgaa tgccgggttg gcggccagcg gaaagaccgc ggccaccacc
      361 gcgacgacga ccagcacgta cgcagtgacg acgccacgtc gaacggcgcg cgcgggttgc
      421 gcgacctcgc caccgaagaa gaacgggagg cttccgcaga cgaagagcaa ggccacattg
      481 ccggacgcgg acgcgatgcc gccgacgtcc gacgttgtcg tgaatgacga catcggatgt
      541 gcgtgagcaa tcgtgacgac ggcgagcaat ccgacaagaa caagctggcc cacggcgaat
      601 atcgacagca ccaccaccgc tggcgatcgg ccagcgatga caaggatcgc gagcgcgatc
      661 ggaatcgcca cttccagcgt gctgcgatat ggctgcacgc ccgggatcac cgttggcagc
      721 acgtcgtaga cgacgtagac ggtggtgtac acgagataga gcaggtagct gacggtccag
      781 atcgttgcct gcaccagcgc gacgcggcga ccggccgctg cctcgacgaa actgaagagc
      841 ccacccgacg acgagatctc gcgcgcgtag cgcaaccaga tcagcagcgg aaacgcgaag
      901 gccagcggag cgatgacagt gacgagaccg gccgaactga aaacgtcgtc gacaagcccc
      961 ggaacgtaga gcgcagcgag cgcaagtggc ccgccgaacg aaacgactgc gatggcgagg
     1021 aaaccgcccg ggctcagcgt tcgctgaagc ccgggcggtg ccgcgccgtt aagaccagcc
     1081 gccaccagca gctgccgatg acttgctggc agtcggcgac ggggccgctg ccgccggatt
     1141 ggtctttgac gccgcagagg ccttcgccgc aggtgcgctg ccgatctggg caccagccgg
     1201 tgacaccaag aaccaggtgc cttccttgcc ctgtcctgtc gtgtcaccaa ccgacttgtc
     1261 gcccttgaag aagtaaagcg ggtgactctt gtaggtcacc tgcatcgtcc cgtcggtccg
     1321 catcgtcgtg ccgagctggc tggcctctgc gccactaccg gcttggggcg ctgaactggt
     1381 gagaagcggt ggccagaagg cgatgcactg cccgtagcag gtcgaggtcg acgacgtgtc
     1441 tggcgtgtag aggtacagcg tgtggcctgt ggcgtcggtc aggtaggtgc caagcggccc
     1501 cgtctttaca tccacggctg ccggcccggc ggctggggcc gcacttgcgc cgaccgctgc
     1561 accactcgca gcggcaggag ctactccggc gacgctcgtc gcagccgcgc ttggcgctgg
     1621 ggttccggcg gctgccgcag ccgcagtcgt ggctggggcg ctgttcgtgg ctgcggtatt
     1681 ggatttcttg ctagcgcagc cggccatcag cagcgcggcc aatccaagaa agaccgtcgc
     1741 cttgatcact ggtgctgtcg ttcgcgtgac gttgttcatg tctgggctcc cgcgtgatgg
     1801 ttggctcttc gatccccacc acggctcccg tcggcgtccc gttcaaatca ttagatttcg
     1861 ttttaggcac gcttctgatc gcggccccta aggtgggtca atgcccaaag acagcgcggc
     1921 cggctcgaca ctgctcggtt cgctcgtcgg ctcactcgac gccgcaccgc cctaccttct
     1981 cgccgaggtg ctgcacaact tcttgaacgc ggaggtcggc gcgcgctctt cgccggtctt
     2041 gctcgcagac tacgaggagc gcatgctcga gccggtacct ggtggcgcgc cgtcccccac
     2101 gttgggggca caacagatcg acggcagcca agccggccgc gcgtaccgcg aacagcgact
     2161 ggtcgagatc gaggcgagcg gcgaggtgac gctctatctg ccgataacgc agcgagcgga
     2221 acgggtcggc gtcctcgaga tcacgatgcc gacaccaggc atcgacgtgc ggcagcagct
     2281 caccgaggtc gcccgcattc tcgcttattt catcgccgct gcccgccggt acaccgatca
     2341 gttcga
//
LOCUS       DASWVF010000183         6074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_387245, whole genome shotgun sequence.
ACCESSION   DASWVF010000183 DASWVF010000000
VERSION     DASWVF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387245"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VGD55_03200"
     CDS             complement(<1..378)
                     /locus_tag="VGD55_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HEY0869381.1"
                     /translation="MHDVAIGLGYTGATLGVAMVVPQIIRIIRHPSLTGVSPLSWALS
                     TFGCLAWLTYGLRTGATPQIPGNILLASGATVCVLLINSPMKRGRRGALLLVGAAVLL
                     VTVWVIPAESVGDLATVSGLSSMG"
     gene            538..1599
                     /locus_tag="VGD55_03205"
     CDS             538..1599
                     /locus_tag="VGD55_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0869382.1"
                     /translation="MAGSAEPPAARPTLETVARAAGVSPMTVSNAYNRPDQISEQTRA
                     RVLEVARELGYPGPDPAGRSLRRRRADTIGVLLTEQLPYAFADPGMVAFLHGVATGLA
                     DAGQALLLLPTEGNAEFAHVRNALVDGFVVTSLAPGHPAVADVLGRRLPVVSWGYPRI
                     TGVPRIGVDNARAVAKAARHLLELGHTSFGIVTLGDPSDPSADVPGVELAMRSRVSGF
                     LKALAAGGVDSASVRVVGAARNARPHGFEAARSLVAGRLRPTAVFGVTDILALGVLDA
                     AAAAGVSVPGELSVVGFDDVAAAATSVPPLTTVSQSLFDQGKLAARVVLDQVAGREVK
                     IPPIRTELVVRGSTAPLHA"
     gene            complement(1621..4830)
                     /locus_tag="VGD55_03210"
     CDS             complement(1621..4830)
                     /locus_tag="VGD55_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869383.1"
                     /translation="MRARSFTPATGHRFAGSARRVGVAATVVAVGLGGVIAAAPPSVA
                     LPPAGPTVTVLNNLSNPASNVAVGATLAVTAAAPITVPGATAQTITQTWNTAQASITP
                     SSIVTPEGWTTTYTTDGTTYSSTLPSPSTSISGVQSTGAVFSDGTSSGLQVSTATGTG
                     TLKTAPVFAPSSGGDGWDAFTSGAFVMNAWHHNSAAYNLDCHLVSTGTACGPVYSVSG
                     YETSKASSGTVDSGKVYSVVGENASSSIGVLCTNVSALPFTSCGYTMLVAGTADYNLI
                     GSAQESGTKIYAPLSTGSVVCFDISTLAACAGQPYALSSFGTSGSSIPAFATAVGPDV
                     FITASKIWCLTGSTGAACAGSWPAGSYSTSVLTGAVPMRNTSGTLTGVCMIAPAGTCF
                     TLAGASATPPTGFAALLASNGPDAAVGVGGWGQFGFGATKQYWFAGAWGNPNTPVCYD
                     WTTDAACAGFQTTTAIGVIRYTIRVDTTNPNCLWSNGDNGSVAPFDANTGQPGCQGSD
                     PVVDIPYTAVVPRLSCTEAGRIRNWQNITFTPPGAIAVSALRVTVLDSSGVAIAGYTA
                     LTPNGSGVVDLSALAVATSGTQPTFEVTAIGASSPNASAISAAVTYTSDAPQLCFTLT
                     TIHNCPTLAPGLSLAATVAVSDVALGGSTVATASGVPTTTPLSAAITRANMAGCVGTV
                     SGTATQTYSSGAPDAPLAYGTVNLLDSLGTVVASTMTASDGTYSFANANPAAYTAQMS
                     QVTHALSVSAGGTTVQNFSVPYVAPTVAPAPMFTQLGGADRDTTAAMVATTMYPLVGS
                     ASVVVLARDDLYADGLTGSPLANALGGPLLLSPTASLSAATQGAVGHVLVPGGLVIVL
                     GGTSAISPSVVTTLQKLGYQVERIGGADRFATATLIADKIGTKHAVTHVYVATGFNFA
                     DALSAADAAGLNNGVVLLTANGTMPGSTSTWLAAHAGAAATAIGGQAAAADPTATALV
                     GSDRYATAALVANSVAPNTVGIVLATGANFPDGLAGAVYAAHNGWSLLLVDPTAVALN
                     TDQMSYLHEVSATVSTVTAIGGTATMPVAATTLITAGLT"
     gene            4987..6033
                     /locus_tag="VGD55_03215"
     CDS             4987..6033
                     /locus_tag="VGD55_03215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0869384.1"
                     /translation="MTAQDLWRRHEDGVFMRFAAPPGWPQPPAGWQPPPGWLPDPTWP
                     PAPYGWRFWSEPTSPRRRKGFRGWAASMLPPTPTPRRPTISVSPRRAWAEVLGVFAMF
                     FLAGVLAAVFYDAHHDVNPTTISVYDGILAAISHLAIAAMAIVVVCSLTRLRGLSFSD
                     IGLAPAWRTHRAYRWQGFGVGMMFIGAVTVSALLLHLVSPHAKYPFLPVAVWHLIYEI
                     PAALEAGIVEELVVVALLVTALEQARTKVWLIFVVGIALRLSYHVYYGPGVIVFVLWA
                     AAAIWLFRRTRRITPLIVAHALYDAFGAFAHEVPHPPFVIGELLGLVIIALPIVVIVR
                     AIQIALRGRRPAGR"
BASE COUNT          952 a         1949 c         2119 g         1054 t
ORIGIN      
        1 ccccatcgac gagagtccgc tcaccgtcgc gaggtcgccg acgctctcag cgggaatgac
       61 ccagaccgtg accagcaaca cggccgcgcc gacgagaagc agcgcccctc ggcgtccgcg
      121 cttcattgga gaattgatga gaagcacgca cacggtcgct ccggatgcga gcaggatatt
      181 gccgggaatc tgcggcgtgg caccggtgcg caggccgtat gtcagccagg cgaggcagcc
      241 gaacgtcgac agcgcccacg acagcggcga caccccggtc agcgacgggt ggcgaatgat
      301 gcggatgatc tgcggcacca ccatcgcgac tcccagagtc gcacctgtgt atccgaggcc
      361 gatcgccacg tcgtgcatgc tcactgcaag aactcctcaa cccggacgcc gaccagctgg
      421 cgatcaacgc gacttcaact atcttcggca caagacgatg ctttctgtaa cgatacagtt
      481 cctgggctaa cggcggccta acggcgtcgg tcgcagacca ttaagagggg acggcgaatg
      541 gctggttcgg ctgagccacc tgctgcgcgt ccgactctgg agaccgtcgc gcgggcggct
      601 ggagtgtcgc cgatgacggt gtcgaatgcg tacaaccggc ccgaccagat ctcggagcag
      661 acccgcgcgc gggtgctcga ggtggcgcgc gagctggggt atccggggcc tgatccggcc
      721 gggcggtcgt tgcgccggcg tcgggccgac acgatcgggg tgttgctgac cgagcagttg
      781 ccgtatgcgt tcgccgaccc cgggatggtg gcgttcttgc acggggtggc gaccgggctt
      841 gccgatgcgg ggcaggcgtt acttctgttg ccgaccgagg ggaacgctga gttcgcccac
      901 gtgcgcaacg ctctggtcga cgggttcgtg gtgacgtcgt tggcgccagg acatccagcg
      961 gtcgccgatg ttttgggtcg ccggttgccg gtggtctcgt gggggtaccc gcgcatcacc
     1021 ggggtgccgc gcataggtgt cgacaacgct cgggccgttg cgaaagctgc gcggcacctg
     1081 ctcgagcttg gtcacacgtc gttcgggatc gtcacgctgg gggatccgtc cgatccgtct
     1141 gccgacgtgc ccggcgtgga gctggcgatg cggtctcggg tgtcgggatt cttgaaagca
     1201 ttggcggccg ggggcgtcga ttcggcgtcg gtcagggtgg tcggcgctgc ccgcaacgcc
     1261 cgaccacacg gcttcgaagc agctcggtcg ctggtcgccg gccggcttcg cccgacggca
     1321 gtgttcggtg tcactgacat cctcgcgctc ggagtcctcg acgcagctgc ggcggctggt
     1381 gtctcggtgc cgggagagct gtcagtggtg gggttcgacg atgtcgcggc cgccgcaacg
     1441 agtgtgccgc cgctcacgac ggtgtcacag tcgctgttcg accagggcaa gctggcggcg
     1501 cgagtcgtgc tcgatcaggt cgcgggccgt gaggtgaaga tcccccccat ccgcaccgag
     1561 ctagtagtcc gcggcagtac cgcgccgcta catgcctgat caagaacgga ctagtccggg
     1621 ctacgtcagc ccggccgtga tgagtgtggt ggccgcgacc ggcatcgtcg ccgtcccgcc
     1681 gattgcagtg acagtcgaca ccgtggcgct gacctcatgc aggtagctca tctggtcggt
     1741 gttcaacgcg accgccgtcg ggtcgaccag caacaacgac caaccgttgt gcgctgcgta
     1801 gaccgcgccg gcgagtccgt cggggaagtt cgcgccagtg gccagcacga tgccgacagt
     1861 attcggagcc accgagttcg ccacgagtgc tgcggtcgcg taccggtcgc tgccgaccaa
     1921 cgccgtcgcg gtcggatcag ccgccgctgc ctgaccgccg atagcggtag ccgctgcgcc
     1981 ggcgtgtgcg gcgagccagg tgctcgtcga tccgggcatg gtgccgttcg cagtgagcag
     2041 cacgacaccg ttgttgagcc ccgcggcgtc ggctgctgac agcgcgtcgg cgaagttgaa
     2101 cccggtcgcg acatacacgt gcgtgaccgc gtgcttcgtg ccgatcttgt cggcgatgag
     2161 cgtggcagtc gcgaaacggt cggcgccacc gatgcgctcg acctggtagc cgagtttttg
     2221 cagcgtcgtg acgacagaag gactgatcgc gctcgtgcca ccaagcacga tcaccaaccc
     2281 gccgggcacg agcacgtgac cgacggcgcc ttgggtagcg gccgacaatg atgcggtcgg
     2341 gctcagcaac agcggtccgc cgagcgcatt ggccaacggg gagccggtca gcccgtcggc
     2401 gtacagatca tcacgagcca gcacgacgac gcttgccgag cctacgagcg gatacatcgt
     2461 cgtagccacc atcgccgcgg tggtgtcacg gtcggcgcca ccgagctgcg tgaacatcgg
     2521 tgcgggtgcg acggtcggcg cgacgtaggg aaccgagaag ttctgcacgg ttgtgccgcc
     2581 ggcgctgaca ctgagcgcat gtgtgacctg cgacatctga gcggtgtaag cagcggggtt
     2641 cgcgttggcg aagctatacg tgccgtcgga ggctgtcatc gtcgaagcca cgacggtgcc
     2701 taacgaatcg agcaggttca cggtgccgta ggcgagtgga gcatcgggcg caccgctcga
     2761 ataggtctgg gttgccgtgc cgctgaccgt gcccacgcag cccgccatgt tcgctcgcgt
     2821 gatggcggcc gacaacggag tcgtagtcgg cacgcccgag gcagtggcta cggtcgatcc
     2881 gccgagcgcc acgtcagaga ccgcaactgt cgcggcgagc gagaggccgg gtgccagcgt
     2941 cgggcagttg tgaatcgtgg tcagcgtgaa gcacagctgc ggtgcgtcgg aggtgtaggt
     3001 caccgccgcg ctgatggcac tcgcgttcgg cgacgacgcg ccgatggcgg tgacctcgaa
     3061 ggttggttgc gtgcctgatg tggcgacagc caacgccgag aggtcgacga ctccgctgcc
     3121 gttcggtgtc agagcggtgt agcccgcgat cgcgacgccg gaagagtcca gaaccgtgac
     3181 ccgcaatgcg ctcaccgcga tagcgcccgg cggtgtgaac gtgatgttct gccagtttct
     3241 gatgcggccc gcttccgtgc agctcagccg cggaaccact gcagtgtaag gaatatcgac
     3301 gaccggatct gacccttggc agccgggctg accggtgttg gcgtcgaagg gcgccaccga
     3361 gccgttgtcg ccgttcgacc acaggcagtt ggggttggtg gtgtcgaccc tgatcgtgta
     3421 ccggatcacg ccgatcgccg tcgtagtctg gaaaccggcg cacgcagcgt cggtggtcca
     3481 gtcgtaacat accggcgtgt tgggattgcc ccacgctccg gcgaaccagt actgcttggt
     3541 cgcaccgaag ccgaactggc cccagccacc aacgccgacg gctgcgtcag gcccgttact
     3601 ggcaagcaga gcggcgaaac ccgtcggcgg tgtggcgctc gcgccggcaa gcgtgaagca
     3661 ggtacccgcg ggcgcgatca tgcagacacc ggtgagcgtg cccgacgtgt tgcgcatcgg
     3721 gacggcgccc gtcagcacgc tcgtgctgta cgagccggct ggccacgatc ctgcgcacgc
     3781 cgcaccggtc gagccggtca ggcaccagat cttgctggcg gtgatgaaca cgtcgggccc
     3841 gacagcagtg gcgaacgcgg ggatcgagct gccgctggtt ccgaatgacg acaacgcgta
     3901 cggctggccg gcgcaggccg ccagtgtcga gatgtcgaaa cacacgacgc tgccggtgct
     3961 gagaggcgca tagattttgg tgccggactc ttgtgcagac ccgatgaggt tgtagtcggc
     4021 cgtgccggcc accaacatcg tgtacccgca cgaagtgaac ggcagcgccg acacgttggt
     4081 gcacagcacc ccgatcgagc ttgaggcgtt ctcgccgacg accgagtaga ccttgccgga
     4141 atcaaccgtt ccgctgctgg ctttcgacgt ctcgtagccg ctgacggagt aaaccggtcc
     4201 gcacgcggtg cccgtcgaca cgaggtgaca gtcgaggttg taggcggccg agttgtggtg
     4261 ccacgcgttc atcacaaacg cgcccgaggt gaaggcgtcc cagccgtcgc cacctgacga
     4321 aggcgcgaac acgggcgcgg tcttcaatgt gccggtgccg gtcgcggtcg agacttgcag
     4381 gccactcgac gtcccgtcac taaaaacagc acccgtcgat tgcaccccgc tgatgctcgt
     4441 cgagggtgac ggtagcgtcg agctataggt cgtgccgtca gtggtgtagg tcgtcgtcca
     4501 gccctccgga gtcacgatgc tcgacggggt gatgctcgcc tgcgccgtgt tccaggtctg
     4561 ggtgatcgtc tgcgcggtcg cgccaggcac cgtgatcggc gcagccgctg tgacggccag
     4621 cgttgctcca accgccacgt tggacgccgg gttgctgagg ttgttcaaca ccgtgacggt
     4681 cggtccggcg ggtggcagcg cgacggacgg cggggcagcg gcgatcacgc caccgagccc
     4741 gacggcgact accgtggccg cgacgccaac acggcgggca gacccggcga agcgatgacc
     4801 ggttgcggga gtgaacgacc gtgcgcgcat gcgaactcct ggctcgatgc actcacgcgg
     4861 cgtgagcgct ccatgccctt tcggctggcg aaggcgaacg cgaaggaatt cgggcaggct
     4921 tctgggggac gccgactggt gcggcccgtt cgggccgttt agctgcgatc gggtttcgcc
     4981 gatgccatga ctgcgcagga cttgtggcga cggcatgagg acggggtgtt catgaggttc
     5041 gctgcgccac ctggctggcc acagccaccc gccggttggc agccgccacc gggatggctg
     5101 cccgacccga cctggccgcc ggcgccttac ggctggcggt tttggtccga gcccacctcg
     5161 ccgcgccgcc ggaagggctt tcgcggctgg gcagcgtcga tgctgccgcc gacgcccacc
     5221 cctcggcgtc cgaccatctc tgtttcacct cgccgcgcct gggctgaggt gctcggagtc
     5281 ttcgcgatgt tcttcctcgc cggagtactc gctgccgtct tctacgacgc gcaccacgac
     5341 gtcaacccga cgacgatctc ggtgtacgac ggcatcctgg ccgccatctc gcacctggcg
     5401 atcgctgcga tggctatcgt cgtggtctgc tcgctgacgc ggctacgcgg gctcagcttc
     5461 tccgacatcg gcctggcgcc cgcgtggcgt acgcatcgcg cgtaccgctg gcagggattt
     5521 ggcgtcggga tgatgttcat cggcgcggtg acggtgtcgg cgttgttgct gcatctggtc
     5581 agcccgcacg cgaagtaccc attccttccg gtggcggtgt ggcatctcat ctatgagatc
     5641 ccggccgcgc tcgaggcggg catcgtcgag gaacttgtcg tcgtcgcgct gctcgtcacc
     5701 gcgctcgagc aggcgcgcac caaggtctgg ctcatcttcg tcgtcggcat cgcgcttcgg
     5761 ttgagctatc acgtctacta cggtccgggc gtcatcgtgt tcgtgctgtg ggcggccgcg
     5821 gcgatctggt tgttccgccg cactaggcgc atcacgccgc tgattgtcgc gcacgcgctc
     5881 tacgacgcgt tcggggcgtt tgctcacgag gtaccacacc cgccatttgt gatcggggaa
     5941 ttgctcgggc tcgtcatcat cgcgctgcct atcgtcgtga ttgtgcgggc gattcagatc
     6001 gccttacgcg gacgccgacc agctggccgc tagggcctta ggcgatcaga tcgagtggtg
     6061 gcaaccgcgc ccga
//
LOCUS       DASWVF010000184         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_489027, whole genome shotgun sequence.
ACCESSION   DASWVF010000184 DASWVF010000000
VERSION     DASWVF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489027"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGD55_03220"
     CDS             <1..337
                     /locus_tag="VGD55_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY0869385.1"
                     /translation="GLPIVVGGRSSGARVACRTAAATGALGVIALAFPLHPPGKPEKS
                     RVAELDAGVPVFVLQGDRDPFGGPSDFPSGVTVCEVTGADHRLAGPALPAAIEAALAW
                     LAALLTTSP"
     gene            628..1242
                     /locus_tag="VGD55_03225"
     CDS             628..1242
                     /locus_tag="VGD55_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0869386.1"
                     /translation="MATVEESTQERSARFEQSAMPFLDQLYSAALRMTRNPADAEDLV
                     QETFAKAFAAFHQFEEGTNLKAWLYRILTNTFINSYRKKQREPKQAGGDELEDWQLAR
                     AAEHTSSGLRSAETEALDHLPDSDVKEALQALPEEFRVAVYLADVEGFAYKEIADIMG
                     TPIGTVMSRLHRGRRALRASLEDYALERGVTRGPVGAVDGGGEQ"
     gene            1239..1553
                     /gene="rsrA"
                     /locus_tag="VGD55_03230"
     CDS             1239..1553
                     /gene="rsrA"
                     /locus_tag="VGD55_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEY0869387.1"
                     /translation="MSCGKHHETPCTEVLDQVYGYLDGEIDAAECEKIRGHLEECGPC
                     LRQYDLDKVVKALVARSCGCDLASDELRGKVLARIRAVQIEISEGAIVGIDVSQTEFR
                     AD"
     gene            complement(1576..2268)
                     /locus_tag="VGD55_03235"
     CDS             complement(1576..2268)
                     /locus_tag="VGD55_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869388.1"
                     /translation="MPALRLLPELEEALRQVPGIRAASVVTGPDAVPTEIHIVASRSK
                     GAKQVVRDVQSLAMAGYDIDIDHRIVSVVQFDDDIDGLQHDDDLHTIIRLPDSAPAIA
                     APDILPRPVIAAITIRTAGAEADASVVVRTGDATYDGRSIGPSTMSHRHTLIARATLD
                     AISELLGLPAEVEFVTVSTMGTRRMAACVIQVAVPGIGELVLTGSALVRSDEADAVAR
                     AVLDALNRRLAG"
     gene            complement(2243..2761)
                     /locus_tag="VGD55_03240"
     CDS             complement(2243..2761)
                     /locus_tag="VGD55_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869389.1"
                     /translation="MAVNEPADDGHANDRMAALVAALLGVRIDHASVRFDAEVDAALA
                     ANRIDVQTARALRFWQRASVQAVEEYIGAALPGALTARDDADLQGSIDAAQAAAAWEQ
                     SQAALPTQPAERAPVVTPTQLRRRLVSLRVSPPSHRSAKETEQAVRAALQFAEMPFEG
                     KDSGHARSATSA"
     gene            3526..4917
                     /locus_tag="VGD55_03245"
     CDS             3526..4917
                     /locus_tag="VGD55_03245"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEY0869390.1"
                     /translation="MRDMPRRAQAYVCAVICAGIVVVAFAATSGSVAWADVAVLGALY
                     WFADSRQISTRAGALGVSASSPVALASWLLLGPRGAALVCSLSALSYARNLAWFKHAF
                     NGAQAALSALIAGTVYHAIPGSATSGFAHAAFPRVLPSVAVAGLSYYVLNNLFVAVVV
                     RLTTDTSMRAVWWNGVAEMAVPTLGYGSIGLIMAVLWVGGVGAVAALLVLAPLMIARW
                     AFEQYSEQHAAYDATVRSLIQAVETKDYYTRGHSERVAKASVLIAQQLGMREDRLEML
                     RYAGMLHDVGKLGVPTKLLQKTGPLDDEEFASIKLHPTRGYEMVNGISFLEEATLGIR
                     HHHERVDGRGYPDGLAADEIPEFARLIAVADAFDSMTSTRSYRGARSVEAAIRELRRW
                     AGTQFDGVMVEALVVAVGISGWEASVDATDDIVDPAMLVFRDHDDPSGDVALGRAAAM
                     HDAYGLRDEQQVS"
     gene            4919..>5963
                     /locus_tag="VGD55_03250"
     CDS             4919..>5963
                     /locus_tag="VGD55_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0869391.1"
                     /translation="MTALPGGTSDSAGSEVVGRVLVAIVCVGSIVAAVATALSGWVRP
                     EVAVAFIALITFGELLGVRLAGDRPSAPIASAAALGYAMLFDVGGQPVSQSVAQVVTV
                     TAAGCTLAAVIRVVAGRRPRLTAVARRIVLAAVVATVFRLTAPQRLTTLHFAAFKSQL
                     RWVAFLMVAVVALATVLDFVIAAFVRARGRAASFGWTLRDEISATAALSAAVGATGVL
                     TALATPSMQLYALPVFCIPLLMTQFAFRRYASIRTTYRQTIRSLSRVTEVGGYVEEGH
                     SHRVSQLAIAIGRELGLTENDLLELEYAALLHDIGQLSLIDPIPGGSTLVVAPVERRR
                     VAELGADVIKTTGV"
BASE COUNT          933 a         1955 c         1979 g         1096 t
ORIGIN      
        1 aggtctgccg atcgtcgtcg gcggccgcag cagcggggct cgggtcgcgt gtcgaacggc
       61 cgctgccact ggtgctctcg gtgtcatcgc actcgccttt cccctgcacc cgcccggcaa
      121 gccggagaag agccgggtgg cagagctcga cgcgggtgtt cccgtgtttg tgttgcaggg
      181 cgaccgagat cccttcggcg gcccatcgga tttcccgtct ggtgtcacgg tgtgtgaggt
      241 caccggcgcc gatcaccggt tagccggccc ggcgctgccg gctgccatcg aggcggcgct
      301 cgcgtggctg gcggccctgc tcaccaccag cccgtgaccg aggacccgga tttgcgagtc
      361 cggggaatcg ggccgttaca gcccgcgttg tggcctgtcg agagtcaatg accctcaaga
      421 ccccgatgtg ctggcgagag gtggcggcag gtgcaagcgg cccagaagcg aacgagcggt
      481 gattcgccgc tgcgctggcc agactggccc ctcaccccga ccgacggatc ggtcaactcc
      541 gaccgaccgg tagtgtcgat gagcagcccc gcaacatcgc accgcccagc tgttcgtgcc
      601 atcagcacat cacagggagc ccggtctttg gccaccgtcg aggagtcgac gcaggaacgc
      661 agcgcacgct tcgagcaaag tgcgatgccc ttccttgacc agctgtattc ggctgcgctg
      721 cgcatgacgc gcaatccagc tgatgccgaa gatctcgtgc aagagacctt cgccaaggcg
      781 tttgcggcct tccaccagtt cgaggaaggc accaacctca aggcgtggct ctaccggatc
      841 ctgacgaata ccttcatcaa cagctaccgc aagaagcagc gcgagcccaa gcaggccggc
      901 ggggacgaac tcgaagattg gcagcttgcg cgcgcggccg agcacacgtc gtccggcttg
      961 cgctctgctg agaccgaggc tctcgaccac ctgcctgact ccgacgtgaa agaggcgctg
     1021 caagcccttc ccgaagagtt ccgggtcgcg gtctacttgg cggacgtcga ggggttcgcg
     1081 tacaaggaga tcgccgacat catgggcaca ccgatcggca cggtcatgtc gaggctgcac
     1141 cgcggccggc gcgcgctgcg cgcttccctc gaggactacg cgcttgagcg cggcgtcacg
     1201 cgcggcccgg tcggtgccgt cgacggcggt ggcgagcagt gagctgcggc aagcaccacg
     1261 agaccccgtg caccgaagtc ctcgaccagg tctacggcta tctcgacggg gagatcgacg
     1321 cggccgaatg cgagaagatc cgaggccacc tcgaagagtg cggcccgtgc cttcgccagt
     1381 acgacctcga caaggtggtc aaagctctcg tcgcgcgtag ctgcggttgc gatctggcgt
     1441 cagacgagct gcgcggcaag gtgctcgcgc gcatcagggc ggtacagatc gagatttccg
     1501 aaggcgcgat cgtcgggatc gacgtgtcgc agaccgaatt ccgcgctgac tagagcgcgc
     1561 gacccagcag gcaggttagc cggcgagtcg ccggttgagc gcgtcgagca cggcgcgcgc
     1621 gaccgcgtca gcttcgtcgc ttcgcaccag cgcagacccg gtcagcacca gctcgccgat
     1681 ccccggcacc gcgacctgga tgacgcaggc agccatccgg cgcgtgccca tcgtggagac
     1741 ggtgacgaac tcgacttcgg caggtaaccc cagaagttcg ctgatcgcat cgagggtcgc
     1801 tcgagcaatg agagtgtgcc ggtggctcat agtcgacggg ccgatcgagc ggccgtcgta
     1861 ggtagcgtcg ccggtgcgca cgacgaccga agcgtcggct tccgcacccg cagtgcgaat
     1921 ggtgatcgcc gcgatcaccg gtcgcggcaa gatgtcgggc gcagcgatgg caggtgcgga
     1981 gtccggcagc cggatgatcg tgtgcaggtc gtcgtcatgt tgcagaccgt cgatgtcgtc
     2041 gtcgaactgc acgacgctca cgatgcggtg gtcgatgtcg atgtcgtagc cggccatcgc
     2101 cagcgactgc acgtcgcgga ccacttgttt cgcgcccttg cttcggctgg ccacgatgtg
     2161 gatctcggtc ggcaccgcgt cggggccggt cacgacactc gccgccctga tgcccggcac
     2221 ctggcgaagc gcctcctcga gttcaggcag aagtcgcaga gcgggcatgg ccgctgtcct
     2281 ttccctcgaa tggcatctca gcgaactgga gggcggctcg cacggcctgc tcggtttctt
     2341 tcgccgaccg gtgcgacggc gggcttacgc gcagggatac gagtcgtctc ctcagctgag
     2401 ttggcgttac tacgggggcc cgctcggctg gctgggttgg cagcgccgcc tgcgactgtt
     2461 cccacgccgc cgctgcttgc gccgcgtcga tggatccctg aaggtcggcg tcatcgcggg
     2521 cagtgagcgc gccgggtagt gcggcgccga tgtactcctc gaccgcttgc accgaagcgc
     2581 gctgccaaaa ccgcagggca cgcgcggtct gcacgtcgat ccggttagct gcgagcgcgg
     2641 cgtctacctc ggcatcgaac cggacgctcg catgatcgat ccggacgccg aggagcgcgg
     2701 cgacgagggc cgccatgcga tcgtttgcgt ggccgtcgtc ggcgggctcg ttcacagcca
     2761 aagcgcaacg cctttctgcc accggttctt cacgggccgc cgtcccactg gccacatccg
     2821 tcacttgccg cacctgcgac gcagcggggc gagtctacga ccgagtcatc gtcgtgcggg
     2881 agtacctggg ataggcaata tgcacctctt gcacagttcg cgtgcatgca aataaggagg
     2941 taatcggatt aataggatga acccctatat cccgtcgccg tcatacaact ggcattcgag
     3001 ctagtccttt atgactggcc tggaaggagt gggccgagac ccgtgtggtt gtagtgtcca
     3061 acgggtcttg acttgccctg atcttgaccg agtcactcct tatacggacg gccgtttcac
     3121 cccgacttgt gggcgcagcg gccgccgtgt gcaccccggg aaccccccaa tttcccgggt
     3181 tgctaacaac tgcagagcga acgggacttg ggcgtatttg gccgattttc tactccttta
     3241 gcggagcgta tctcgcatag gctttagcgg cgcctatgac gtgattcgta ggagttgaga
     3301 tccacgtgaa gaagctctct gctgtggtcg aaaagttctt gctcggcgcc acgacgcact
     3361 ggaactaact aggaaccaac tgggaactaa cagggaacag cttgaaccca ggtgaccagg
     3421 tcccgcttcg ctctgcatat tcgtgcacct gcgtgctctt cctgctcttc ccagctttct
     3481 gctcttcctg ctcttccccc cggcacgtcc ccggcaggag acaccatgcg agacatgccg
     3541 cgccgcgcgc aggcatacgt ctgcgctgtc atctgcgcgg gcatagtggt cgtcgccttc
     3601 gctgcgacct cgggctcagt tgcgtgggcg gacgtcgcgg tcctcggcgc gctgtactgg
     3661 ttcgcggatt cccgccagat cagcacccgt gcgggcgcac ttggcgtgtc tgcgagttcg
     3721 ccggtcgcac ttgcgtcgtg gctcttgctc gggccgcgag gcgccgcact cgtgtgttct
     3781 ctaagtgcgc tctcgtacgc gcgcaacctc gcttggttca agcacgcatt caatggcgcg
     3841 caggcggcgc tcagcgcgtt gatcgccggc accgtctatc acgcgattcc cgggtcggcc
     3901 acgagcggat tcgcgcacgc tgcgttccct cgggtgctgc cctccgtcgc cgtggcgggg
     3961 ttgtcgtact acgtcctgaa caacctgttc gtagcagtgg ttgttcgcct gacgaccgac
     4021 acctcgatgc gtgccgtctg gtggaacggc gtcgccgaga tggcggtccc gacacttggg
     4081 tacggctcga tcgggctcat catggccgtg ctgtgggttg gtggcgtcgg tgccgtggct
     4141 gccctgctcg tgctcgcgcc gctgatgatc gctcgctggg ccttcgagca atacagcgag
     4201 cagcatgcgg cctacgacgc tacggtgcgc tcgctgatcc aggcggtcga gaccaaggac
     4261 tactacaccc gcggtcacag cgagcgggtc gccaaggcat cggtgctcat cgcacagcag
     4321 ttgggtatgc gtgaggatcg cctcgagatg ctgcgctacg cagggatgct gcacgacgtc
     4381 ggcaagctcg gcgtcccgac caagctcttg caaaagaccg gcccgttgga cgacgaagag
     4441 ttcgcgtcga tcaagctgca cccgactcgt ggctacgaaa tggtcaacgg catctcgttc
     4501 ctcgaggaag cgacgctggg catcaggcat caccacgagc gggtcgacgg ccgcgggtac
     4561 cccgacggcc ttgccgccga tgagataccg gaattcgctc gcctgatcgc ggtcgcagac
     4621 gcattcgact cgatgacatc gacgcggtct taccgcggtg cccggtcagt cgaggcggcg
     4681 attcgcgaac tccggcgatg ggccggcacc cagttcgacg gagtcatggt cgaagcactc
     4741 gttgtcgcgg tcggcatcag cggctgggaa gccagcgtcg atgcgaccga cgacatcgtc
     4801 gatcccgcga tgctcgtctt ccgcgatcac gacgatccgt ccggagacgt cgcgcttggg
     4861 cgcgcggccg caatgcacga cgcgtacggc ttgcgcgacg agcaacaggt ctcgtgacgt
     4921 gacggctcta ccggggggaa ccagcgacag cgcgggctcc gaggtcgtcg gccgcgtgtt
     4981 ggttgcgatc gtctgcgtgg gctcgatcgt cgctgctgtg gcaacggcgc tttccgggtg
     5041 ggtacgcccc gaggtcgcgg tcgcgttcat cgcgctcatt accttcggcg aactgctcgg
     5101 tgtccggttg gcgggtgacc ggccgtctgc cccgatcgca tcggcggcgg cactgggcta
     5161 cgcgatgttg ttcgacgtcg gcggccaacc tgtctctcaa tccgttgccc aggtcgtcac
     5221 cgtcaccgcg gccggatgca cgttggccgc ggtgatcagg gtcgtggctg gtcggcgtcc
     5281 acgcttgact gctgtggccc gacgcattgt gctggccgca gttgtagcga cggtgttcag
     5341 gctcaccgcg ccgcagcggc tgaccaccct gcatttcgca gccttcaaat cgcagctgcg
     5401 ctgggtggcg ttcttgatgg tcgccgtcgt cgcgctcgcg accgtgctcg acttcgtgat
     5461 cgcggcgttc gtccgcgccc gcggccgggc cgcatcgttc ggctggacgc tgcgggatga
     5521 gatcagcgcc accgccgcgc tttcggccgc cgtcggcgcg accggcgtgc tgactgcgtt
     5581 ggcgacgccg tccatgcagc tttatgcgtt gccggtcttt tgcatcccgc tcctgatgac
     5641 tcagttcgcg tttcgccggt acgcgtcgat ccgcacgacc taccggcaga cgatccgctc
     5701 gttgtcgcgg gtcacagagg tcggtgggta tgtcgaggaa gggcactcgc accgggtcag
     5761 ccagctcgcg atcgcgatcg gtcgcgaact cgggctcacc gagaacgacc tgctcgaact
     5821 ggagtacgcc gcgctgctcc acgacatcgg ccagctgtcg ctgatcgatc cgatcccggg
     5881 cggctcgacc ctggtggtcg caccggtcga gcgccggcgc gtcgccgaac tgggcgccga
     5941 cgtcatcaag acgaccggcg ttc
//
LOCUS       DASWVF010000185         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_5023, whole genome shotgun sequence.
ACCESSION   DASWVF010000185 DASWVF010000000
VERSION     DASWVF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VGD55_03255"
     CDS             <1..1237
                     /locus_tag="VGD55_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0869392.1"
                     /translation="EFLLDVNYTVSQNNPIDHTVVGSTTLSRNNEMTLQFLGFGGDFH
                     YEHARGRLMTQFGMRSTVVPRNDGSTFRGQFDMQTALRYVSEAYGGYHWDALHGINLD
                     AGIFMSYVGLFSYDNAENWMYLPSYTSDNTPWFFNGMRLQIFTSDKLKIEPWLINGWQ
                     TYGKFNEMPGFGAQILYRPVEWISMLSNDYTGWDTQDHPGRVRFHSDNSFLMRYFNVP
                     DQKGVFTRAAFSITADIGGEQGDGVTPFGGHHDSTGTGASCTVASPCQQQFLSWMAYN
                     RLWFFDGHLGWTTGGGMMHNPGRYLVLPPTGVATPYAPDSAQGISNTLPGSQGYSLNP
                     GTTFDAWDVASGIQWMPDEITTWDLEFNHRQAADPYFAGHGGVTSPDGYITTSVPAGW
                     RPDLVKSDNRIIAALLVRF"
     gene            1256..1582
                     /locus_tag="VGD55_03260"
     CDS             1256..1582
                     /locus_tag="VGD55_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869393.1"
                     /translation="MKTSTLAKHLASLAATFAVVLLACNQGSEGDRCNPDLSHDECGS
                     GLSCTTPVDCPESYCCASDGKSTNPYCQRGCNGGQASICASGGSADCWQLEDGGVPPE
                     DSGGGG"
     gene            complement(1589..>1797)
                     /locus_tag="VGD55_03265"
     CDS             complement(1589..>1797)
                     /locus_tag="VGD55_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869394.1"
                     /translation="LDMTMPELSGEETFRELRAIRADVPVILSSGYNEIEATRRFASK
                     GFAAFLPKPFTPDDLARCIRAALA"
BASE COUNT          316 a          636 c          569 g          276 t
ORIGIN      
        1 cgagttcctg ctcgacgtga actacaccgt ctcgcagaac aacccgatcg atcacaccgt
       61 cgtcggctcg acgacgctct cgcgcaacaa cgagatgacg cttcagtttc tcgggttcgg
      121 cggcgacttc cactacgagc acgcgcgcgg gcgactcatg acccagttcg gcatgcgctc
      181 gacggtcgtg ccccgcaacg acggcagcac gttccgtggg cagttcgaca tgcagaccgc
      241 gcttcgctac gtgagcgaag cgtacggcgg ctaccactgg gacgcgctgc acggcatcaa
      301 cctcgatgcc ggcatcttca tgtcgtacgt cggcctgttc tcgtacgaca acgccgagaa
      361 ctggatgtac ctgccgtcgt acacctcgga caacacgccg tggttcttca acggcatgcg
      421 ccttcagatc ttcacgagcg acaagctgaa gatcgagccg tggctcatca acggctggca
      481 gacgtacggc aagttcaacg agatgccggg cttcggcgcg cagatcctct accgccccgt
      541 cgagtggatc tcgatgctct cgaacgacta cacgggctgg gacacgcagg accaccccgg
      601 gcgcgtccgc ttccacagcg acaacagctt cctcatgcgg tacttcaacg tgcccgatca
      661 gaagggggtc ttcacgcgcg ccgccttctc gatcaccgcc gacatcggcg gcgagcaggg
      721 cgatggcgtg acgccgttcg gcgggcacca cgactccacc ggtacgggcg cgtcgtgcac
      781 cgtcgcgagc ccgtgccagc agcagttcct cagctggatg gcctacaacc ggctctggtt
      841 cttcgacggt cacctcggat ggacgaccgg cggcggcatg atgcacaacc cgggtcgcta
      901 cctcgtcctg cctccgacgg gcgtggcgac gccctacgcg cccgacagcg cgcaggggat
      961 cagcaacacc ctcccgggtt cgcagggcta cagcctgaac cccggcacga cgttcgacgc
     1021 ctgggacgtc gcgtcgggca tccagtggat gccggacgag atcaccacgt gggaccttga
     1081 gttcaatcac cgacaggcgg ccgacccgta tttcgcgggg catggcggcg tcacgtcgcc
     1141 ggacgggtac atcacgacct ccgtgccggc cgggtggcga cccgacctcg tgaagtccga
     1201 caaccgcatc atcgccgcgc tcctcgtgcg cttctgagaa cgagaggaca agaccatgaa
     1261 gacttccact ctcgccaagc acctcgcctc gctcgccgcg acgttcgccg tcgtcctgct
     1321 cgcctgcaac cagggctcgg aaggcgaccg ctgcaacccg gacctgtcgc acgacgagtg
     1381 cggcagcggc ctgtcgtgca ccacgcccgt cgactgcccc gagagctact gctgcgcgag
     1441 cgacggcaag agcacgaacc cgtactgcca gcgcggctgc aacggcgggc aagcgtcgat
     1501 ctgcgccagc ggcgggagcg cggactgctg gcagctcgag gacggcggcg tgccgcccga
     1561 ggactcgggc ggcggcgggt agacccgctc aggccagcgc ggcgcggatg caccgcgcga
     1621 gatcgtccgg cgtgaagggc ttcggcagga acgccgcgaa gcccttcgac gcgaacctcc
     1681 gcgtcgcctc gatctcgttg tagccgctcg acaggatcac gggcacgtcg gcgcggatcg
     1741 cgcggagctc ccggaacgtc tcttcgcccg agagctccgg catcgtcatg tcgagga
//
LOCUS       DASWVF010000186         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_30440, whole genome shotgun sequence.
ACCESSION   DASWVF010000186 DASWVF010000000
VERSION     DASWVF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30440"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VGD55_03270"
     CDS             <1..1152
                     /locus_tag="VGD55_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY0869395.1"
                     /translation="LWFGLASTAQWLTHACGIAQRTAVEHVRVAGSLAQFPQLAAEMG
                     AGRLSFSQVRAISRTATPGEHGLVADLVEAARHATVAQLEVVVRGLRTVDHNEPATDP
                     GESLTQSWTSDSRWQLSARLDPERGAVVALEAIVQRDGCTPAEALERLAQICLASLAD
                     AGDPPRDRRGDEHAAVVIHLDAARVRPRSAEPDPGDVSSVARPYARVAGGPGLPDRVI
                     QRLLCDGRIRTVIHDRGSNMLDVGRSHRLVTDRQYRALLLRHHGHCAHPGCPNAKNLH
                     AHHRIHWIHGGRTDLDNLLLLCERHHLAHHGGEFELFTLGAGRFRFVTSDGRDLSAPL
                     RSTASPNTPPLEDEHAQVAADAATPRWDGQRLDRPYAISVLAQRRQAAS"
     gene            1207..1350
                     /locus_tag="VGD55_03275"
     CDS             1207..1350
                     /locus_tag="VGD55_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869396.1"
                     /translation="MAQWRIELTTQDLVALERGMEVVVSAIDEEPDASEDQIDVTISL
                     LEV"
     gene            complement(1384..2025)
                     /locus_tag="VGD55_03280"
     CDS             complement(1384..2025)
                     /locus_tag="VGD55_03280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869397.1"
                     /translation="MHEDEHGHRPGAPVPADEWDARYSEQDALWSGRPNGRLVAEVGA
                     LAPGRALDVGCGEGADAIWLARAGWTVTAIDISDVALGRARAAADRVDAVVEWICGDA
                     LQVALPASSFDLLSMQYPALPKAAGEAAMRALLDTVRPSGLLLAVYHDLDDEHREHMK
                     SRGFDPAEYIDADDLGQLLGDDFTIELQAIVPRIDPPPDNPHIADVVLRARRD"
     gene            complement(2062..2952)
                     /locus_tag="VGD55_03285"
     CDS             complement(2062..2952)
                     /locus_tag="VGD55_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0869398.1"
                     /translation="MSRVLIAGDVIDDIVVTPLDTLTRDSDTTSHIRHTPGGSAANQA
                     TWLASRGVTVRFVARVGAADVERHRSELESFGVEAVLIPDHDAPTGTIVVLLDGDGNR
                     TMFTDRGANLRLTTADLPPSLLDGVDALHVNGYAIFADEPRSAMTTLVAEALRRDIVV
                     TVDPCSSAYIERLGASTFLACTLGATACMPNLDEGRALTGCSEPVDVLSFLRDHYPVV
                     ALKLGSGGVLVSERGRVEMIPSVASSVVDPTGAGDAFCAGFLASWLGGHSAGDAAREG
                     SVLAAHAVAVLGGRPPADGS"
     gene            complement(2992..3927)
                     /locus_tag="VGD55_03290"
     CDS             complement(2992..3927)
                     /locus_tag="VGD55_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY0869399.1"
                     /translation="MAPLVKISDEVSAAIAEGRPVVALESTIIAHGLPRPQNLQAAHD
                     FEAILTDAGVVPATIAVIDGVPTVGLDADELERIAIEDIPKVSSRDLAVAVGRKSSGA
                     TTVAATSTLAALAGIRVFATGGLGGVHRAASESFDESADLVTLSRTRVTVVCAGVKSI
                     LDIAATLERLETLNVPVVGYGTNQFPAFYLSDSGFSLEWRVDSPEEVAAIMRAGDELG
                     SRGALIVANPVPLDRQLDPDVHDRLLIQALHAADEAGLRGKAVSPFLLDYFQRESGGR
                     SLVVNLDLARNNVAVAGGIAIAWAALPTDTSAASA"
     gene            complement(3929..>4689)
                     /locus_tag="VGD55_03295"
     CDS             complement(3929..>4689)
                     /locus_tag="VGD55_03295"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEY0869400.1"
                     /translation="PELHVERGLSLDAVVEAVLEGFRLGSEGRGIRIGVLLTALRHAA
                     RSFEIAELAIRYRDAGVVGFDIAGAEAGYPPTRHLDAFQYMQRENGHFTIHAGEGFGL
                     PSIWEALQWCGADRLGHGVRIVDDIMAVDGEPVLGRLAAYVRDNRVPLEMCPSSNVQT
                     GAAASIVDHPIGLLRKLYFRVTVNTDNRLMSGTSVGTEFAKLVDAFGYEIDDVRWFTI
                     NALKSAFIPFDERLALINDVVKPRYAALRDAQEV"
BASE COUNT          841 a         1583 c         1550 g          715 t
ORIGIN      
        1 ctgtggttcg ggttagcatc gaccgcgcag tggttgaccc atgcgtgtgg gatcgcgcag
       61 cgcaccgcgg tcgagcatgt gcgggtcgcc ggatcgctgg cacagttccc ccaactcgcg
      121 gcggagatgg gcgcgggccg gttgtcgttt tcgcaggtcc gcgcgatcag ccgcaccgcc
      181 acacccgggg aacacggtct ggtcgccgac ctggtcgagg cggcccggca cgccaccgtg
      241 gcccagctgg aggtggtggt ccgcgggctg cgcaccgtgg accacaacga gcccgccacc
      301 gacccaggtg agtccctgac ccagtcgtgg acctccgaca gccgctggca actctcagca
      361 cgccttgacc cggaacgcgg cgcggtggtg gccctggagg cgatcgtgca acgcgacggc
      421 tgcaccccgg cggaagcact ggagcgcctc gcgcagatct gcctggcgag cctggccgat
      481 gcaggcgacc caccgcgcga ccggcgcggt gacgaacacg ccgctgtggt catccacctc
      541 gacgccgcgc gggtgcggcc gcgttcagct gaacccgacc ccggcgacgt gtcgagcgtc
      601 gcgcggccct acgcgcgggt cgcgggtggg ccgggtcttc ccgaccgggt catccagagg
      661 ctgctgtgcg acggccggat ccgcaccgtg atccacgacc gtgggtccaa catgcttgac
      721 gtcggccgat cccaccgcct ggtcaccgac cggcaatacc gcgccctgct gctacgccac
      781 cacggtcatt gcgcccaccc gggctgcccg aatgcgaaga atctgcacgc gcatcaccgc
      841 atccactgga tccacggcgg ccgcaccgac ctggacaacc tgctactcct atgcgaacgc
      901 caccacctcg cccaccacgg cggcgagttc gaattgttca ccctcggcgc gggcaggttc
      961 cggttcgtca cctccgacgg ccgcgacctc agcgcacccc ttcgaagcac ggcaagccca
     1021 aacaccccgc cccttgaaga cgagcacgca caggtagctg ctgatgccgc cactccccga
     1081 tgggacggcc aacgactcga ccgcccctac gccatcagcg tcctcgccca acgccgacaa
     1141 gctgccagct gacaacgcaa cccggtgcgc acaattgcta gacgtactag ccaataggga
     1201 gcgtcgatgg cgcagtggcg aatcgagttg accacccaag atctagtggc actcgagcga
     1261 ggcatggagg tcgtggtctc ggcgattgac gaggaacctg acgcctccga agatcagatc
     1321 gacgtgacga tcagtctgct cgaggtataa gcgcgccccg actaacgagc acgaggtcgg
     1381 aggtcagtca cgccgcgcgc gcagaacgac gtcggcaata tgcgggttgt ctggcggcgg
     1441 gtcgatgcgc ggcacgatcg cctgtagctc gatcgtgaag tcgtcgccga gcagctggcc
     1501 gaggtcgtct gcatcgatgt actccgcggg gtcgaagccg cgcgacttca tgtgctcgcg
     1561 atgctcgtcg tctaggtcgt gatagacagc gagcagtaac ccactgggcc gcaccgtgtc
     1621 gagcaacgcc cgcattgctg cctcgccagc tgccttcggc aacgccggat actgcatcga
     1681 cagcaggtcg aacgagctgg ctggcagcgc gacctgcaac gcgtcgccac agatccactc
     1741 gaccaccgca tcgacccgat ccgccgccgc ccgcgcccga cccagcgcca cgtcggagat
     1801 gtcgatcgcg gtcacggtcc agccggcgcg agctagccag atcgcgtcgg cgccctcgcc
     1861 acaaccgacg tcgagtgccc ggccaggggc gagggcgcca acctcggcga cgagccgtcc
     1921 attcggccgg ccgctccaca gcgcatcctg ctcgctgtac ctcgcgtccc actcgtcggc
     1981 cggcacgggc gcgccgggcc ggtggccatg ctcgtcctca tgcatgcttt gcagcgtagt
     2041 gacgaatgtt gaaaagcggt gctagctgcc gtcagcaggg ggacggccgc cgagcaccgc
     2101 gactgcatgg gccgcgagta ctgagccttc gcgcgcggca tcgccagccg agtggccgcc
     2161 gagccacgag gccagaaaac ctgcgcagaa cgcgtcgccc gcaccggtcg ggtcgacgac
     2221 tgacgacgcg actgacggaa tcatttcgac ccgaccacgc tcgctgacca gaacgccacc
     2281 cgatccgagc ttcaaggcca caaccggata gtggtcgcga aggaagctga gcacgtcgac
     2341 gggctcggag cagccggtga gcgcgcgacc ttcgtccagg ttgggcatgc acgcggttgc
     2401 gccgagcgtg cacgcgagaa atgtcgatgc accaagcctt tcgatgtagg ccgacgagca
     2461 tgggtcaacc gtgacgacga tgtcgcggcg cagcgcctcc gcgaccaacg tggtcatcgc
     2521 tgaccgcggt tcgtcggcga agattgcgta gccgttgacg tgcaatgcat cgacaccatc
     2581 gagcaacgac ggtggcagat cggcagtggt caggcgcagg ttggcgccac ggtcggtgaa
     2641 catcgtgcgg ttgccatcgc cgtcgagcag caccacgatc gtgccggtgg gggcgtcgtg
     2701 gtcagggatc agcaccgcct cgaccccgaa cgattcgagt tcgctgcgat ggcgctcgac
     2761 atcggccgcg ccgactctcg cgacgaatcg gacggtgaca ccgcgcgacg cgagccacgt
     2821 cgcttgattt gccgcagagc caccgggcgt gtggcggata tgcgacgtgg tgtcactgtc
     2881 gcgggtgagc gtgtcgagcg gcgtaacgac gatgtcgtcg atgacgtcgc cggcgatgag
     2941 caccctagac atggacagca tgaacagcgt cgagagtcgg cagcattggc gtcaagcaga
     3001 ggccgcggag gtgtcggtgg gcagcgccgc ccacgcgatc gcgatgccgc ctgccacggc
     3061 gacgttgtta cgggcgaggt cgaggttgac gaccagcgac cgaccaccgc tctcgcgctg
     3121 gaagtagtcg agcaggaacg ggctcaccgc cttgccacgc agacccgctt cgtcggccgc
     3181 gtgcagtgcc tggatcagca accggtcgtg aacgtcggga tcaagctggc gatccagtgg
     3241 caccgggttc gcgacgatca gcgcgccgcg cgaaccgagt tcgtcgcctg cccgcatgat
     3301 ggcggcgacc tcttcggggg aatcgactcg ccactcaagg gaaaacccag aatcgctgag
     3361 ataaaacgcc gggaactgat tggtgccgta gcccacgacg ggaacgttga gagtctcaag
     3421 gcgttcgagg gttgccgcga tgtcgaggat cgacttcact cctgcgcaca ccacggtgac
     3481 acgggtgcgc gacaacgtca cgaggtcggc tgactcgtcg aacgactcgc tcgcggcgcg
     3541 atgcacacct ccgagacctc cggttgcaaa cacgcggata cctgcgagag cggcaagagt
     3601 cgaggtggcc gccacggtgg tggcaccgct ggacttgcgc ccgaccgcga cggcgaggtc
     3661 acggctgctg accttcggaa tgtcttcgat ggcgatgcgt tcgagttcgt cggcgtcgag
     3721 ccccacggtc gggacgccgt cgataaccgc gatcgtggcg ggcacgactc cggcgtcggt
     3781 gaggattgct tcgaagtcgt gcgcagcctg gaggttctgc gggcgaggca ggccgtgggc
     3841 gatgatcgtc gactcgagag cgacgacagg tcgaccctcg gcgatggcgg cactgacctc
     3901 gtcggagatc ttcacgagtg gtgccatgtc atacctcctg agcatcgcgc aacgcggcgt
     3961 acctgggctt gacaacgtcg ttgatcagtg cgagccgctc gtcgaacggg atgaacgcac
     4021 tcttcagcgc gttgatcgtg aaccaccgca cgtcgtcaat ttcataaccg aaagcatcga
     4081 cgagcttcgc gaactcggtg ccaaccgacg tcccgctcat taagcggttg tctgtattca
     4141 cggttacccg aaagtagagc ttgcgcaaca ggccaatcgg gtggtcgacg atggaggctg
     4201 ccgcgccggt ctgaacgttc gacgacgggc acatctcgag cggcacgcgg ttgtcgcgaa
     4261 cgtaggccgc caggcggccg agcacgggct cgccgtcgac cgccatgatg tcgtcgacga
     4321 tccgcacgcc gtggccgagg cgatcagcac cgcaccactg gagagcctcc cagatcgacg
     4381 gcagcccgaa tccctcaccg gcgtggatcg tgaagtgccc gttctcgcgc tgcatgtact
     4441 ggaacgcgtc gaggtgacga gtcggtggat acccagcttc ggcgccggca atatcgaatc
     4501 cgacgacgcc ggcgtcgcga tagcggatcg cgagctcggc gatctcgaac gagcgcgccg
     4561 cgtgccgcaa cgccgtgagc aacacgccga tcctgatgcc gcgaccctcg ctgccgagcc
     4621 ggaatccttc gagcacggct tcgacgacgg cgtcgagact aagacccctc tcgacgtgca
     4681 actctggtg
//
LOCUS       DASWVF010000187         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_55880, whole genome shotgun sequence.
ACCESSION   DASWVF010000187 DASWVF010000000
VERSION     DASWVF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55880"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1660)
                     /locus_tag="VGD55_03300"
     CDS             complement(197..1660)
                     /locus_tag="VGD55_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869401.1"
                     /translation="MTEGAAPQGWRRLLIEPRRPLAVRNWPRAPWLVVVTVSIGAFLG
                     QLDASIVTLALPTLQTHFHASLAAVEWVALSYLLVLVASVAAVGRLADMIGRKLLYTY
                     GFAVFTLASVGCGLSPTLGVLIGFRIVQAIGAAMLQANSVALIATAIPRASLGRAIGI
                     QGAAQAVGLALGPSVGGFLIGAAGWRSIFFVNAPVGVVGLVLGLLLLPRSRDLAPRVP
                     FDWVGLGLFCPAIVAFLVALNIVGHSTAARSLLLISASLICVALFVRRERRTPSPLID
                     PRLFRNIDFTAGVASGLLSYLVLFGTLFVVPVYLEHAQHMSPTRAGLELGVLPVALAV
                     VAPFAGRSRDRVGARVPTVTGMLVTAVLLAALAVGGRSSVWIVVLLAGIGAGLGLFTP
                     ANNAAIMAAAPRDHSGMAGGVLNLTRGLGTALGVAVTGLTLGLRNAERPLASAHAVTT
                     GFQTCAAVLAVVALAAAGLASLRAPRPSSAMGPDSSD"
     gene            1724..2188
                     /locus_tag="VGD55_03305"
     CDS             1724..2188
                     /locus_tag="VGD55_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0869402.1"
                     /translation="MPAAARASNTDAEALTDVVNRLRRVLRAGIRIDYPWETLPMAQI
                     ELLQCLRDHEPAGIRVGEICTRLHLAPATTSGLVQQLVEAGTARRQPDPADRRASVVR
                     LTTAGRRQLQDWQQAHERRLGNALSQLSVSQRAAVRAAVPALAALVDKLSRS"
     gene            complement(2198..2566)
                     /gene="crcB"
                     /locus_tag="VGD55_03310"
     CDS             complement(2198..2566)
                     /gene="crcB"
                     /locus_tag="VGD55_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY0869403.1"
                     /translation="MLLALGVAIVSGVGAVCRYVADQLISHKHGALFPWGTFAINVVG
                     SFVLGLSTGLATHHGLSTTAAAIIGSGFCAGFTTFSTWMWETLALAETGAFLKAGLNA
                     FGSLAIGLTAAGCGLWLALL"
     gene            complement(2566..3129)
                     /locus_tag="VGD55_03315"
     CDS             complement(2566..3129)
                     /locus_tag="VGD55_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEY0869404.1"
                     /translation="MAPNFSAEGWEAIAPDGTDHLHGLPVDPDIADPVEPTPIGPSTR
                     QIPYLPPAVLVAIFVGGVLGGLTRYGIGRAWPPRSGGFPWDVFAINIFGAFALAMLLG
                     LVKELWPRTRYVRPAVGTGFLGAFTTFSSLAVASDQLLAHGHPVLAIGYIAGSLFGGV
                     AAAALGLLVARILSTMRHRVPTAAGTL"
     regulatory      complement(3113..3180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3258..3536
                     /locus_tag="VGD55_03320"
     CDS             3258..3536
                     /locus_tag="VGD55_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869405.1"
                     /translation="MHSQPASDTATWSNVGAVKHGYPPPRLSVGALTTAYCGAELIVE
                     GECSDRPPLDACALCALAWENSKRPGRRWARALRAEPGTKRLGDPTAE"
BASE COUNT          777 a         1250 c         1225 g          504 t
ORIGIN      
        1 cccggatctg cttgcccgcc aactcatgat cgttgtcgac ggcgcgcagg ctcagtcggt
       61 catcgagcac acgtcaaagc cagctcatga cgcacgtggg atcgcgaacg cacttattgc
      121 ggctgcgatc ccctagcgag cgctgcttgg cgccgcagga gcgtgctcga ctcggtcgtg
      181 tcgtaggcag catctttcaa tcgcttgaat ccgggcccat tgcgctggaa ggtcggggcg
      241 cccgaagcga ggcgagaccg gcagcagcga gtgcaactac cgccaagacg gccgcgcagg
      301 tttggaatcc ggtggtgacg gcatgtgctg acgcgagcgg ccgttcggcg ttgcgcaacc
      361 ccaacgtcaa accggtgacc gcaacaccta gtgccgtgcc aagaccacgg gtgaggttca
      421 acacgccacc ggccatgccg gaatggtcgc gaggtgccgc ggccatgatg gccgcattgt
      481 tggcaggcgt gaacaaaccg agccctgcac ctatacccgc gagcaacacg acgatccaca
      541 ccgagcttcg accacccacc gcgagcgccg ccaacagcac ggcggtgaca agcatgccgg
      601 tgacagtcgg gacccgggca ccaacccgat cgcgcgaccg tcccgcgaac ggtgcaacaa
      661 cggcgagcgc caccggcagc acgccgagtt cgagccccgc acgagtaggc gacatgtgct
      721 gggcatgctc gagatagacg ggaaccacga agagcgttcc gaagagcacg agatagctga
      781 gcaggcccga cgcgacgcca gccgtgaaat cgatgttgcg aaacaggcgc gggtcgatca
      841 gaggcgaggg tgtgcgtctt tcacggcgaa cgaataaggc cacgcagatc agtgacgcgc
      901 tgatcagcaa cagcgaacgc gctgccgtgg agtggccgac gatattcagc gcgactagga
      961 acgccacgat ggcaggacag aaaaggccga ggcccaccca gtcgaatggc acccgcggcg
     1021 cgagatcacg cgaacgtggc agcagcagca aaccgagcac cagaccgacg acgccgaccg
     1081 gggcgttgac gaagaagatc gaacgccacc cagccgcgcc gatcaggaac ccaccgacgc
     1141 tcggacccaa tgcaagacca acagcttgcg cggccccttg gattccgatg gcacgcccga
     1201 ggctggcccg tggaatcgca gttgcgatca gcgccacgga attcgcctgc aacatggccg
     1261 caccgatcgc ttgaacaatg cgaaagccga tcaagacgcc gagggtgggc gagagtccgc
     1321 agccaaccga cgcaagcgtg aagaccgcga agccgtaggt gtagagcagc ttccgcccga
     1381 tcatgtctgc gagccggccg accgccgcca cgctcgcgac cagcaccagc aggtagctca
     1441 acgccaccca ctcgacggcg gccaggcttg cgtgaaagtg cgtttgcaac gtagggagcg
     1501 caagagtcac gatcgaggcg tcgagctggc caagaaacgc gccgatgctc accgtgacga
     1561 cgaccaacca cggagcccgc ggccagttgc ggactgccaa cggtcgccgc ggctcgatga
     1621 ggaggcgacg ccatccctgt ggagcggcgc cttcggtcac ggtctcaaaa tacaacggtt
     1681 accgaacatt tcggaaggcg gcgcattgcc ttaggatcag tgcgtgcccg cagccgctag
     1741 agcgtcaaac acagatgccg aggcgttgac cgacgtagtc aaccggcttc gtcgcgtgct
     1801 gcgcgcgggt atccgcatcg attacccatg ggaaaccctg ccgatggcgc agatagaact
     1861 gctgcagtgc ctgcgggatc acgaaccggc cggaatcaga gtcggggaga tctgcacccg
     1921 gctacatctc gcgcctgcga caacgagcgg cctcgtgcag cagcttgtcg aggcaggcac
     1981 cgcgagacgg caacccgatc ctgccgatcg gcgcgcctcc gtggtgcgat tgacgacggc
     2041 cggacgccga cagctccagg actggcagca ggctcacgag cgccggctcg gaaatgcctt
     2101 gagccagttg tcggtctccc agcgggcagc ggtgcgcgct gctgttcctg cactggcggc
     2161 gttggtcgac aaactcagtc ggagctgagc ccgcgttcta caacagcgcc agccacaatc
     2221 cacagcctgc ggcggtcagg ccgatcgcca gactaccgaa agcgttgagc cccgccttga
     2281 ggaatgctcc ggtttcggca agcgccagcg tctcccacat ccacgtcgag aaggtggtga
     2341 acccggcgca aaacccgctg ccgatgatcg cggcggcagt ggtcgacaga ccgtggtgcg
     2401 tcgccaggcc agtactcaat ccaaggacga acgacccgac aacgttgatg gcgaaggtgc
     2461 cccacggaaa cagggcgccg tgcttgtgcg agatcagttg gtcggcgacg tagcggcaca
     2521 cggcaccaac accggagacg atcgcgacac caagcgcaag cagcatcaga gggttcccgc
     2581 agccgtgggc acccgatgcc gcatcgtact gagaatgcga gcgaccaaga gccccaatgc
     2641 agcggccgca acgcccccga agaggctgcc tgcgatgtat ccgatggcga gcaccgggtg
     2701 gccgtgcgcc agcaactgat cactggcgac ggcgagcgag gagaacgtcg tgaacgcccc
     2761 gaggaaaccg gtaccaacag ccggccggac gtagcgggtc cgcggccaca gctctttcac
     2821 caggccgagc agcatcgcca acgcgaacgc cccgaagatg ttgatcgcaa agacgtccca
     2881 gggaaatccg cccgaccgag gcggccacgc gcggccgatc ccgtagcgag tcaggccgcc
     2941 gaggacgcca ccgacgaaga tggcgacgag caccgcggga ggcaagtacg ggatctgacg
     3001 ggtcgaggga ccgatcggtg tcggctcgac cggatcggcg atgtccgggt ctacgggcag
     3061 gccgtgcagg tgatccgtgc cgtccggggc gatggcttcc cacccctccg cggagaagtt
     3121 aggagccatc agccggacgg cggtttgact acgcaggcga gctccatcgc cacattcacc
     3181 ctagcccggg caagtctcgt gagtgcgagc acggtccgcg gctcgtactc gtgccgcctc
     3241 tgacgtaccg tggcacgatg cactcgcagc cggcttcgga caccgcgacc tggtcgaacg
     3301 tcggcgcggt gaagcacggc tatccgccgc cgcgcctttc ggttggtgcg ctcaccacgg
     3361 cctactgcgg cgccgagctc atcgtcgagg gcgagtgttc tgaccggcct ccgctcgatg
     3421 cctgtgcgtt gtgcgcgctg gcctgggaga acagcaagcg gcccggacgc cgatgggcgc
     3481 gcgctctccg cgccgaaccg ggtacgaagc gactcggcga cccgacggcc gaatgaccgc
     3541 aggaacattg ccaggcccgg agccactgga gcccgcatgg aaagcacgat tccgtgctgc
     3601 ccgcatgacg ctgcccgact gggccctgct gcggccagaa cgttccgtgt tcgtcagcaa
     3661 cgcgagcggg acgttcgaga tctacggctg ggatcgtgcc accgacacga cgacccaagt
     3721 cacgtcacgg ccagccggaa cccacatcgc caccat
//
LOCUS       DASWVF010000188         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_285213, whole genome shotgun sequence.
ACCESSION   DASWVF010000188 DASWVF010000000
VERSION     DASWVF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285213"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VGD55_03325"
     CDS             complement(<1..967)
                     /locus_tag="VGD55_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869406.1"
                     /translation="MIQVKGLTKYYGEHAAIHDLNFDIQRGEVIGFLGLNGAGKTTTL
                     KILGCVLLPTAGEVTVDGIDIARDPHAVRRRIGLLPDTPPVYGEMTVGRYLKFAAELR
                     GVPAVGAAAAVTEAEEKTALRDKHEELISSLSHGYKQRVGVAQALVHKPKLLILDEPT
                     SGLDPVQIVEMRSMIRALQGEHTILLSSHILSEISQTCDRLLVIQKGEIVAQGSEQDL
                     ATKLGGSGTIEVEVAGTALRAVEVVKTVTGIGPCRILRESGGVALLSIEGSPDLRPKV
                     ARALVQNGIDLLRIDRGAARLESIFLKLTKAPDGATPTTTTAETLP"
     gene            1138..>2443
                     /gene="purF"
                     /locus_tag="VGD55_03330"
     CDS             1138..>2443
                     /gene="purF"
                     /locus_tag="VGD55_03330"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0869407.1"
                     /translation="MIGVYGHPEAANLAYLGLYALQHRGQESAGIVATDGERLRSVKE
                     MGHVNDIFTVDRLRELRGYAAIGHVRYSTAGDSSKRNAQPIAVDYFGGSVAVGHNGNL
                     VNAAELRERLEADGAIFQTTADTEAIVHLIARSREQTLPERVADALRQVRGAYSLVFL
                     TEDQVIAVRDPMGFRPLALGKSKDTWVVSSETCAFELLEADFVRDVEPGEMVIIDSNG
                     VRSERPFVEQASHRCVFEWVYFARPDSTIDGKSVYRARERMGRRLAVEHPVPADVVIP
                     VPDSGVAAAIGYARESGIPYDQGLMRSHYMGRTFIEPSQHIRHFGVKLKLSPVREVLA
                     GKRVVVVDDSIVRGTTSRKIVGMIRGAGAKEVHMRISSPPTVGPCRYGIDTPTAEELI
                     ASNHTRDEIRDYVGADSLGYLSLDGLYASVNGGNDEKRGGFCD"
BASE COUNT          373 a          810 c          835 g          425 t
ORIGIN      
        1 ggggaagcgt ctccgccgtc gtggtcgtgg gggtcgcgcc gtccggcgcc ttggtgagct
       61 tgaggaagat cgactcgagg cgggccgcgc cccggtcgat gcgcagcaag tcgatcccgt
      121 tttgaaccag cgcccgcgcg accttgggcc gcaggtcggg cgagccttct attgatagca
      181 gcgcgacgcc gcccgactcg cgcaggatcc ggcaggggcc gatccccgtc accgtcttga
      241 cgacttcgac ggcgcggagg gctgtccccg ccacctcgac ttcgatggtc ccgctgccgc
      301 ccagcttggt ggcgaggtct tgttcgctgc cctgggcgac gatctcgccc ttctgaatga
      361 ccagcaggcg gtcgcaggtc tggctgatct cggaaaggat gtggctggac agcaggatcg
      421 tgtgctcgcc ctgcagcgcc cggatcatcg agcgcatctc gacgatctgc acggggtcga
      481 ggccgctcgt cggctcgtcg aggatcagca gcttgggctt gtgcaccagc gcctgggcca
      541 cgccgacgcg ctgcttgtag ccgtgcgaca ggctcgagat cagctcctcg tgcttatcgc
      601 gcagcgcggt cttctcttcg gcttccgtca ccgccgccgc cgcaccgacc gccgggacgc
      661 cccgcaactc ggccgcgaac ttcagataac gcccgacggt catctcgccg tagacgggcg
      721 gtgtatccgg caagaggccg attcgccgtc gcaccgcgtg tggatcgcgc gcgatgtcga
      781 ttccgtcgac ggtcacctcg ccggcggtcg gcaggaggac gcagcccaat attttgaggg
      841 tggtcgtctt gccggcgccg ttcaggccga ggaacccgat gacctcacct cgctggatgt
      901 cgaagttgag gtcgtgaatg gcggcgtgct cgccgtagta cttggtcagt cccttgactt
      961 ggatcatggg gggcttgttc ggaatggggg ggcctttttg gcgggtgacg cggcgcgatt
     1021 atgcccgaag tttttaacgg ctcaaatctc ggacgaacga gacttattcc tgaacgccgt
     1081 ttccgaatgg accgcgaggt gcacctgagt gtgctacctt gcacgcgcat gtgcggggtg
     1141 atcggcgtct atggacaccc ggaagcggcg aacctggcgt atctggggct ctacgccctc
     1201 cagcaccgcg gccaggaatc ggcaggcatc gtcgcgaccg acggagaacg gctgcgctcc
     1261 gtcaaggaga tggggcacgt caacgacatc ttcaccgtcg accgcctccg tgagctgcgc
     1321 gggtacgccg ccatcgggca cgtccggtac tcgaccgcgg gcgactcttc caagcgcaac
     1381 gcgcagccca tcgcggtcga ctacttcggc gggtcggtcg cggtcgggca caacggcaac
     1441 ctggtcaacg cggccgagct gcgcgagcga ctggaagcgg acggcgccat ctttcaaacg
     1501 acggccgaca ccgaggccat cgttcatctg atcgcgcgct cgcgcgagca gacgctgccc
     1561 gagcgggtgg ccgacgcgct tcgccaggtc cgcggcgcct actcgctggt gttcctgacc
     1621 gaggaccagg tgattgcggt gcgcgacccg atgggcttcc ggccgctggc gctggggaag
     1681 tccaaggata cctgggtcgt ttcgtccgag acctgcgcgt tcgagctgct cgaggccgac
     1741 ttcgtgcgcg acgtcgagcc cggcgagatg gtcatcatcg atagcaacgg cgtccgcagc
     1801 gaacgtccct tcgtcgaaca ggcgtcgcac cgctgcgtct tcgagtgggt ctacttcgcc
     1861 cgcccggact cgacgatcga cggcaagtcg gtctaccgcg cgcgcgagcg catgggccgg
     1921 cggctggccg tcgagcatcc cgtgcccgcg gacgtggtga ttccggtgcc cgactcgggc
     1981 gtggcggccg ccatcggcta cgcgcgcgag agcggcatcc cttacgacca ggggctgatg
     2041 cggtcgcatt acatgggccg caccttcatc gagccgtcgc agcacatccg ccacttcggc
     2101 gtgaagctga agctgtcgcc cgtgcgtgaa gtgctggccg gcaagcgggt ggtggtcgtc
     2161 gatgactcca tcgtgcgcgg cacgacctcg cgcaagatcg tcgggatgat ccgcggcgcc
     2221 ggcgcgaaag aggtgcacat gcgcatcagc tcgccgccca cggtcggtcc ctgccgctac
     2281 ggcatcgaca cgccgacggc cgaagagctg atcgcctcga accacacgcg cgacgagatt
     2341 cgtgactacg tgggggccga ttcactgggc tacctgtcgc tcgacggcct ttacgcctcc
     2401 gtcaacggcg gcaacgacga gaagcgcggc ggcttctgcg acg
//
LOCUS       DASWVF010000189         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_438270, whole genome shotgun sequence.
ACCESSION   DASWVF010000189 DASWVF010000000
VERSION     DASWVF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438270"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VGD55_03335"
     CDS             <1..978
                     /locus_tag="VGD55_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869408.1"
                     /translation="PAYISASSQSLVIDIVYPGATVPGLILNVNPLPAACTMAAGVTT
                     CNATVVAQSSAKQFIVTFFDRPNGAGNALSTSTIAAPPAPNGVANISLTLDGVVARVG
                     LSLNGLNYGRPGSAPLIVTAFDADGNVIGGTTPYSGPITLSTSSSALTLSTTTVTSPA
                     TSVTITYNGLADPTLLVFAQPQGKQPIDLQPFGPSPTPLPTPLPSPTPSPTPSPTPTP
                     SPTPTPAPLTAVPPLAPALTQTGPATVTVHETGYRGQYTVSSSNTQVVTVTSPVTSTA
                     DTMAIGITALTSGTANVTVSDSYGQSVVFPVTVTLTPITIQGTHRGGQP"
     gene            975..>2125
                     /locus_tag="VGD55_03340"
     CDS             975..>2125
                     /locus_tag="VGD55_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869409.1"
                     /translation="MNRSLSAVLIAAAVALTACGGGGSSNSAAQVPKSAPSGGVGVLA
                     FSIVVPAKPSTSSQRRKPAYVSPATQSVSFAVGASTPQVVTLSLGSAACPLTSGGYIC
                     TADANVPAGANQSLSIKTYASTDGSGPVLSQNTVLVTVVAAQTNPVTVSLNGVAASLA
                     LSVPGPPVITKCSPASFIAIWSAKDAAGYQIVGPGTIVASDGTPVAPMLTTSDPARFT
                     VGAPSGNVWTVTYTGAGGGASVTLTASNPNVTPATSQQTLNAGSRLFYTTGTNSVSML
                     PPPYTGTATTITNGVIGPTGVLVDSSCNLFVANYGGGGTDTVTEYAPPYSGAPSATIA
                     TTDAFGLAMSAAGDLFVLSISPTSTLSEYAPPYTGAAIATIAFGSPSGPA"
BASE COUNT          362 a          780 c          638 g          345 t
ORIGIN      
        1 ccggcgtaca tttcggcgtc gtcgcagtcg ctcgtgatcg acatcgtgta tccgggcgcg
       61 acggtgcccg gactcatctt gaacgtgaac ccgctgcccg ccgcgtgcac gatggccgcc
      121 ggagtgacga cctgcaacgc cacggtcgtc gcgcaatcgt cggcaaagca gtttatcgtc
      181 acgtttttcg atcgccccaa cggcgcgggc aatgcactgt cgacatcgac aattgcggca
      241 ccgccggcac ccaacggtgt agcgaacatc agcctcacgc tcgacggtgt cgtcgcacgc
      301 gtcggcttga gcctcaatgg cctaaactac ggacggccgg gctcggcgcc gttgatcgtc
      361 accgccttcg acgccgacgg aaacgtcatc ggcgggacga cgccctacag tgggccgatc
      421 actctgagca cgtcgtcttc ggcgctgacg ctctccacga cgaccgtgac gagccccgca
      481 accagcgtca ccatcaccta caacgggctg gcggacccga cgctgctggt gttcgctcag
      541 ccgcaaggga aacagccgat cgatctccag ccgttcggtc cgtccccgac gcccttgccg
      601 acgcccttgc ccagtcccac gccgtcgccg acgccctcgc ccacaccgac gccctcgccg
      661 acgccgacac cggccccgct gaccgccgtg ccgccgctcg cgccggccct gacgcagacc
      721 ggacccgcga cggtcaccgt ccacgaaacc ggctatcgcg ggcaatacac ggtaagcagc
      781 agcaacacgc aggtggtgac ggtcacctcg cccgtcacgt cgacggccga cacgatggct
      841 atcggaatca ccgcgcttac gtccggaacg gccaacgtga ccgtgagtga ctcctacggt
      901 cagtcggtgg tcttcccggt gaccgtcaca ctgacgccga tcacaatcca aggcacgcac
      961 cgcggaggcc agccatgaac cgttcgctgt ccgcggtact gatcgccgcc gctgttgctc
     1021 ttaccgcatg cggcggcggc ggcagcagca actccgcggc gcaggtaccg aagtcggcgc
     1081 ccagcggagg cgtcggcgtg ctggcatttt ccattgtcgt tccggccaag ccgtcgacgt
     1141 cgtcgcaacg gcgcaagcct gcgtacgtct cgcccgcgac gcaatcggtg tcgttcgcgg
     1201 tcggcgcatc gacgccgcag gtcgtgacgc tctcgttggg cagtgccgcc tgcccgctga
     1261 cgtcgggcgg ctacatctgc accgccgatg cgaacgtccc cgctggagcg aatcaaagcc
     1321 tcagtatcaa aacctacgcg agcactgatg gttcaggacc ggtgctctcg caaaacaccg
     1381 tcctggtgac cgtcgtcgcc gctcagacga acccggtcac ggtgtcgctc aacggcgtcg
     1441 ccgcgtcgct cgcactgtcg gtgccgggac cgccggtaat cacgaaatgc agtccagcgt
     1501 cgttcatcgc gatttggtca gccaaagacg cggccggcta tcagattgtc gggcccggta
     1561 cgatcgtcgc ctccgacggt acgccggtcg cgccgatgct gacgacctcg gacccggcgc
     1621 ggttcaccgt cggagcgccg agcggcaacg tctggaccgt cacctatacc ggcgccggcg
     1681 gtggcgcctc ggtgacgttg accgccagca atcccaacgt cacgccggcc acatcccaac
     1741 agacgctcaa cgcggggtcg cggctcttct acacgacggg cacgaactcg gtgagcatgc
     1801 tgccgccgcc gtataccggc acggccacca ccatcaccaa cggcgtgatt ggtccgaccg
     1861 gcgtgctcgt cgatagttca tgcaatctgt tcgtggccaa ttacggcggc ggcggcacgg
     1921 ataccgtcac cgagtacgcg ccgccgtact ccggagcacc gagtgctacc atcgcgacca
     1981 cggacgcgtt tggcctcgcg atgtccgccg cgggtgatct cttcgtgctc agcatatcgc
     2041 cgacgagtac actctccgaa tacgccccgc cctacaccgg agcggcgatt gctacgattg
     2101 ccttcggctc acctagcggc ccggc
//
LOCUS       DASWVF010000190         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_132550, whole genome shotgun sequence.
ACCESSION   DASWVF010000190 DASWVF010000000
VERSION     DASWVF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132550"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="ribB"
                     /locus_tag="VGD55_03345"
     CDS             complement(<1..519)
                     /gene="ribB"
                     /locus_tag="VGD55_03345"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0869410.1"
                     /translation="MTFDTIERAIEEIAAGRAVVVVDDEDRENEGDLIAAASKVTPEI
                     VAFMVRHTSGVLCVPMKSETLDRLGLPPMTARNEDAKQTAYTVSVDARRGVTTGISAA
                     DRAHTIRVLADSATEPYELTRPGHVFPLRAVEGGVLRRAGHTEAAVDLAVLAGLTPAG
                     VISEVVNDDGSMA"
     gene            complement(516..1223)
                     /locus_tag="VGD55_03350"
     CDS             complement(516..1223)
                     /locus_tag="VGD55_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HEY0869411.1"
                     /translation="MSPINWLYTAQWHVGGHAIYYREIFGNLFGLASAVGGMRRRVWA
                     WPIGIVGNVMLFTVFLGSWASHSHQAPLIGQAGRQVFFVITSVYGWYRWAHARRVSSD
                     APAVTPRWATARERVVLFSAAAVLLVVCDVAFHLIGEGFAVQWWYYLADSWIFVGSMV
                     ATYAMARGWTDFWLCWIAVDVVGVPELLHFHYYPSAALYAIYAGFVIWGFFVWRQLQR
                     TAPASRGLSAIPQEATG"
     gene            complement(1220..>1612)
                     /locus_tag="VGD55_03355"
     CDS             complement(1220..>1612)
                     /locus_tag="VGD55_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0869412.1"
                     /translation="QGAGAGTPVNLERAVTLQTRLGGHLVQGHVDGTGAVLGRDHTPE
                     WDVVEISMPQALARYIVEKGSIAVDGVSLTIAAIGAASFTVSLIPTTLKRTTLGDKDV
                     GGEVNLEVDVMAKYAQRLLEFATTKADS"
BASE COUNT          310 a          545 c          499 g          258 t
ORIGIN      
        1 ggccatcgag ccgtcgtcgt tgacgacttc actgatgacg ccggcaggtg tcaggcccgc
       61 gagcaccgca aggtcgactg ccgcctcggt gtgcccggcc cgtcgcagca cgccgccctc
      121 gacggcacgc aaaggaaaga cgtggccggg acgggtgagc tcgtaaggct cggtggccga
      181 gtcggcgagc acccggatgg tgtgggcgcg gtcagcggcg gagatacctg tcgtgacgcc
      241 gcgacgcgca tcgaccgaga ccgtgtacgc cgtctgcttc gcatcttcgt tgcgtgccgt
      301 catcggcggc aaaccgagcc ggtctagagt ttcgctcttc atcggcacgc acaacacgcc
      361 actggtgtgg cgcaccatga aggcgacaat ctctggtgtc acctttgacg ccgccgcgat
      421 gaggtcgccc tcgttctcgc ggtcctcgtc atcaacgacg acgacagcac ggccggcggc
      481 aatctcttcg atcgcgcgct cgatggtgtc gaaagtcatc cggttgcctc ctgcggaatt
      541 gctgatagtc cacgcgacgc cggcgccgtt ctttgcagtt gccgccacac gaagaatccc
      601 cagatcacga accccgcata gatcgcgtac aacgcggctg acgggtagta gtggaaatgc
      661 aacaactcgg ggacgccgac gacatcgacc gcgatccagc acagccagaa gtccgtccag
      721 ccccgagcca tcgcatacgt ggcgaccatc gatccgacga agatccacga gtcggcgagg
      781 tagtaccacc actgcacagc gaagccctct ccgatcaagt ggaacgcgac gtcgcacacc
      841 acgagcaaca ctgcggccgc agagaacaag acgacacgct cgcgagcggt cgcccatcgc
      901 ggcgtcaccg cgggagcgtc gctcgacacc cgtcgggcgt gcgcccagcg gtaccagcca
      961 tacacgctgg tgatgacgaa gaagacctgg cgtccggcct gcccaatcag cggtgcttgg
     1021 tgagagtgcg acgcccaact cccgagaaag acggtgaaga gcatcacgtt gccgacgatc
     1081 ccgatcggcc acgcccagac acgtcgacgc atcccgccga cggcggacgc caacccgaag
     1141 aggttgccga agatttcccg gtaatagatc gcatggccgc cgacatgcca ttgcgcggtg
     1201 tagagccagt tgataggact cacgagtcag ccttcgtcgt cgcgaattcg aggagccgtt
     1261 gcgcgtactt cgccataacg tcaacctcga gattgacctc gccgccgacg tctttgtcgc
     1321 cgagcgttgt gcgtttcaat gtcgtgggga tcaagctgac ggtgaacgag gcggccccga
     1381 tcgccgcgat cgtcaatgac acgccgtcga cggcgatcga gcccttctcg acgatgtagc
     1441 gcgcgagcgc ctgcggcatc gagatctcca cgacgtccca ctcaggcgtg tggtcgcggc
     1501 ccaacaccgc gcccgtgccg tcgacgtggc cctggacgag gtgcccgccc aacctggtct
     1561 gcagagtgac cgcgcgctcg agattcaccg gtgtacctgc acctgcgccc tg
//
LOCUS       DASWVF010000191         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_310667, whole genome shotgun sequence.
ACCESSION   DASWVF010000191 DASWVF010000000
VERSION     DASWVF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310667"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGD55_03360"
     CDS             complement(<1..602)
                     /locus_tag="VGD55_03360"
                     /inference="COORDINATES: protein motif:HMM:NF044554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869413.1"
                     /translation="MPVDVLDPVVGDPMAVSAAGRTLESIAFDIANIASRLRALASGD
                     DSWTGAAAVAAHVRTVALPPSLDKAHTSYAAAGSALGSYARTLAGAQDQSSSAIAAAG
                     RASAELASLVAARTAAEARDASASATALSVGLPRPPATAPRYEASIDEATTQLQRAVA
                     ANDDAHEQQRQAARTAAAALDRASHAGIHNQSWFHHVTHSV"
     gene            complement(665..1339)
                     /locus_tag="VGD55_03365"
     CDS             complement(665..1339)
                     /locus_tag="VGD55_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869414.1"
                     /translation="MTISPDDERRASSATVAPLVPFAPAARQALARASIITPGDWIEL
                     DLDPKTRHTSISRAVRQAVARSRSLAPDAVRLIGLLDRTSRKAAEAGAFYCASRVVED
                     ATGGVLVATVLMQGLKATTPLPAVRPESASPQVGEQCASLAQVMSNDPAWAGANIRVV
                     ALAFIGPAVRVQIEKRGVVVQYVVPLVDGTVDVVLTFSCPCPPYARVMTELFDAMAQS
                     LDLHFE"
     gene            complement(1336..1617)
                     /locus_tag="VGD55_03370"
     CDS             complement(1336..1617)
                     /locus_tag="VGD55_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869415.1"
                     /translation="MPDAFQVTPASLALLASAVGRVRDELSFTADLGGDVGAALGSDV
                     VAGALHHFVTGWHDGRAQICADVGQLADLLTQASAVYAATDGDLAAAMS"
BASE COUNT          327 a          589 c          582 g          266 t
ORIGIN      
        1 accgagtgtg tgacgtggtg aaaccacgac tggttgtgga tgccggcatg cgaggcccgg
       61 tccaacgcgg cggccgccgt tcgcgcagcc tgccgttgtt gttcgtgagc atcgtcgttc
      121 gccgcgaccg cacgctgtag ctgcgtggtc gcctcgtcta tcgacgcttc gtagcgcggt
      181 gcagtcgcgg gcggtcgagg tagtccaacc gaaagcgcgg ttgccgacgc ggacgcatcg
      241 cgagcctcgg cagcagtgcg cgccgcgacc aacgaagcga gctcagcact cgcgcggcca
      301 gccgccgcga tcgcagaact cgactggtcc tgcgcaccag cgagcgtgcg cgcgtaggag
      361 ccaagcgcac tacctgctgc agcgtacgac gtgtgcgctt tgtcgagact cggcggcagc
      421 gccacagtgc gcacgtgcgc cgcgacagct gccgcgcctg tccacgagtc gtctccggac
      481 gctaacgcgc gcagtcgaga ggcgatgttc gcgatgtcga acgcgatcga ctcgagtgtg
      541 cgaccggccg ccgaaacggc catcgggtcc ccgacaacag ggtcgagaac gtcaacgggc
      601 acagcgagaa gttagccgtt tcaacacgat cagcgcgggc ggatgcgcga gccatgtgga
      661 caactcattc gaagtgcaga tcgagactct gagccattgc gtcgaacagt tctgtcatca
      721 ctcgcgcata tggcgggcaa gggcaggaaa acgtgagtac aacatcgacg gtcccgtcta
      781 ccagcggcac aacgtactgc acaacaaccc cgcgtttctc gatctgcact cgtactgcgg
      841 gacctatgaa tgcaagcgcg acgacgcgaa tgttggctcc tgcccacgcc gggtcattgc
      901 tcatcacctg cgcgaggctc gcgcattgtt cgccaacctg cggacttgcg ctctcggggc
      961 ggacggcggg aagcggggtc gttgccttga ggccctgcat gagcacggtt gcgaccaata
     1021 cgccgcccgt cgcatcctcg acgacacgcg aggcgcaata gaacgcgccg gcttcggcag
     1081 ccttcctgct cgtgcgatca agcagaccga tgagccggac ggcgtcgggt gccagactcc
     1141 gtgagcgcgc gacggcctgc ctaaccgcac ggctgatcga cgtgtgccgg gtcttcgggt
     1201 cgagatcaag ttcgatccaa tcgccaggcg tgatgatgct cgcccgggcc agcgcctgcc
     1261 gcgccgctgg cgcaaatggc acgagcggcg ccacggtcgc ggacgacgct cgcctctcgt
     1321 cgtccgggct gattgtcatg acattgctgc tgcgaggtcg ccgtcagtgg cggcgtaaac
     1381 agcggacgcc tgcgtgagca ggtcggccag ctggccgacg tccgcgcaga tttgggctcg
     1441 accgtcgtgc cagcccgtga cgaaatggtg caaggcaccc gccactacgt cagaccccag
     1501 cgccgcgccg acgtccccgc caaggtcggc ggtgaacgag agctcgtcgc ggacccgccc
     1561 aaccgccgaa gcgagcaaag caagggaagc gggtgtgacc tgaaacgcgt ctggcatgca
     1621 atccccccaa caagtcaccc gcgacgctag ccagaatcag cccgcgggaa agccgcgcac
     1681 cggcgacctg tggacaaccc ggcgctcggt aggatcggag gtgccgacca caccgcgaca
     1741 tacgcgcaac gacctgcgag gagc
//
LOCUS       DASWVF010000192         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_489170, whole genome shotgun sequence.
ACCESSION   DASWVF010000192 DASWVF010000000
VERSION     DASWVF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489170"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGD55_03375"
     CDS             complement(<1..379)
                     /locus_tag="VGD55_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869416.1"
                     /translation="MDVQPEFRSDAIWMTIGSEVTLLPVEIQTGVLAGEVTHWLGGSA
                     GVIATIGVRTTPDVAAVCAGQRLWLSGHAALTGELIVLEVIAREASKHVDTSLTLTGV
                     LPLARERRRDSVRAATRHPVRLTF"
     gene            complement(520..1314)
                     /locus_tag="VGD55_03380"
     CDS             complement(520..1314)
                     /locus_tag="VGD55_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0869417.1"
                     /translation="MPAREPAVTGAPNWIDLLSSDTARSKAFYTELFGWTTEEASEEF
                     GGYITFKKNGVQVAGAMARQPENPAPDAWSVYLATDDAAKTLEIATAQGGKVMLEAMP
                     VGDLGVMGFAIDPGGAAIGVWQEGTHKGIGLSDEPGTPAWFELHTRDYDAVVPFYRDV
                     FGWDAQTMSDTPEFRHTVAQDGEAQRAGIMDATAHLPENVPAHWSVYFSVTDTDAALA
                     KAVELGGSVVMPPDDTPYGRLAIATDPTGAHFRLMGPNEAEAAAGS"
     gene            1425..>2381
                     /locus_tag="VGD55_03385"
     CDS             1425..>2381
                     /locus_tag="VGD55_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0869418.1"
                     /translation="MTASTAIPAELEGSFEQHRVELRGYCYRMLASPFDAEDAVQETF
                     IRAWRGYDRFEGRSSLRSWLYKIATNVCLDMMRSVERRARPMDLGPAQEPVLSNLHEL
                     PETSWLEPAPDELIVAAEGDPAEVAVARDSVRLALVAALQHLPPRQRAALILCEVLQW
                     KASEVAELLDTTVASVNSALQRARATLEISKPSASDKTSTLTPADRELLARYVRAFEA
                     YDIDALTALIHEDAIQSMPPYDMWLAGRDDVLAWWFGPGIGCEGSRVIPTLDANGCPA
                     FGQYKPSPEGGYEPWALQVLQLRDGVIGEFTFFLATATLFPLF"
BASE COUNT          362 a          795 c          784 g          440 t
ORIGIN      
        1 cgaaggtcaa tcgcaccgga tgccgcgtcg cagcccggac cgaatcgcgc cgccgttcgc
       61 gagcgagggg caagacgccg gtgagcgtta gcgaggtgtc gacgtgcttc gacgcctcgc
      121 gggcgatcac ctcgagcacg atgagctcac cggtcagcgc ggcgtggccc gagagccaca
      181 gccgctgacc agcgcacacg gccgcgacgt cgggcgtcgt gcgcacgccg atcgtggcga
      241 tgacgccggc cgagccaccg agccagtgcg tgacctcgcc cgcgagcact ccggtctgga
      301 tctcgacggg cagcaacgtg acctcgctgc cgatcgtcat ccagatggcg tcggaccgaa
      361 attccggctg cacgtccacg gttacctccg ggcgggtgcg ggagtctccc acccgacgag
      421 gcaccggcgt ggtctgccct agatgaagtt cctaaggctg gtttacccta ttcaccggat
      481 ttatgcatat actatcttat agttgatgat tccgccgggt cagcttcccg ccgccgcctc
      541 ggcctcgttt gggcccatca gcctgaaatg cgcgcctgtc gggtcggtcg cgatggcaag
      601 ccggccgtag ggggtgtcgt cgggcggcat cacgaccgaa ccaccgagct cgacggcctt
      661 ggccagcgca gcatcggtgt cggtcacgct gaagtagacg ctccaatggg ctggcacgtt
      721 ctcgggcagg tgagcggtgg cgtccatgat cccggcgcgt tgcgcctcgc cgtcctgcgc
      781 aacggtgtgc cggaactcag gagtgtcgct catggtctgc gcgtcccagc cgaagacgtc
      841 gcggtagaac ggcaccacgg cgtcgtagtc gcgcgtgtgc agctcgaacc acgcaggggt
      901 ccccggctcg tcgctgagcc cgatgccctt gtgcgtgccc tcttgccaga cgccgattgc
      961 ggcgccgccg gggtcgatcg cgaatcccat gacgcccagg tcgccgaccg gcatcgcttc
     1021 gagcatgacc ttgccgccct gcgcggtcgc aatttcgagg gtcttcgctg cgtcgtcggt
     1081 cgcgaggtac accgaccacg cgtcaggcgc gggattttcg ggctgccggg ccatcgcacc
     1141 ggccacctgc acgccgttct tcttgaacgt gatgtagccc ccgaactcct cgctcgcctc
     1201 ctccgtcgtc cagccgaaaa gctctgtgta gaacgccttg ctgcgggccg tgtcggagct
     1261 cagcaggtcg atccagttgg gtgccccggt cacggcgggc tctcgtgcgg gcatgtccga
     1321 ctcctttgct cgggttcggg cttactttct tggacgccgc gggtgtagcg aattcatcta
     1381 cgcgcacgta tcgagttgtc ggcgttggtc cctagcatgt gccaatgact gcgagcaccg
     1441 ctatcccggc ggaactagaa ggcagcttcg agcagcaccg agtcgagcta cgtggctatt
     1501 gctatcgcat gctcgcctct ccgttcgacg ctgaagacgc cgttcaagag acgttcatcc
     1561 gtgcttggcg cggctacgac cgtttcgagg gtcgctcgtc gctgcgctcg tggctgtaca
     1621 agatcgcgac caatgtctgc ctcgacatga tgaggagtgt cgagcgccgg gcccggccga
     1681 tggatcttgg ccccgcgcaa gagccggtcc tatcgaatct gcacgagttg cccgagacca
     1741 gctggctcga gccagccccc gacgagttga tcgtcgccgc tgagggtgac ccggccgaag
     1801 tcgccgttgc gcgcgacagc gtgcgactcg cgcttgttgc tgcgttgcag cacctgccac
     1861 cgcgccagcg cgccgcgctg atcctgtgcg aggtcttgca gtggaaggcg agcgaagttg
     1921 ccgagcttct cgacaccacg gtcgcgtcgg tcaacagtgc cttgcaacgg gcgagggcaa
     1981 cgttggagat cagcaagccc tcggcgtccg acaagacctc gacgctcacc cccgcagacc
     2041 gcgagttgct cgcgcgctac gtccgcgcat tcgaggccta cgacatcgac gcgctgaccg
     2101 cgttgatcca tgaagacgcg atccagtcga tgccgccgta cgacatgtgg ctcgccgggc
     2161 gtgacgacgt tctggcgtgg tggttcggcc ccggcatcgg gtgcgagggg tcgcgggtga
     2221 ttccgacgct tgatgcgaat ggctgcccgg cgttcggcca gtacaagccg agccctgagg
     2281 gcggctacga gccgtgggcg ctccaggtgc tgcaactgcg cgacggcgtc atcggcgagt
     2341 tcacgttctt cctcgcgacc gccacgttgt tcccgttgtt c
//
LOCUS       DASWVF010000193         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_107147, whole genome shotgun sequence.
ACCESSION   DASWVF010000193 DASWVF010000000
VERSION     DASWVF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107147"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGD55_03390"
     CDS             complement(<1..379)
                     /locus_tag="VGD55_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869419.1"
                     /translation="MIEFRPAPLDSPVALELIEAVQQEYVVRYGSRDLSELSVDEFTP
                     PQGIFVIGWDVDQAVACGGVRLVTPSVGELKRMYVVPAARRRGIARAMLANLEAHART
                     LGATRLRLETRPASTRGALVVRKR"
     gene            complement(376..1164)
                     /gene="thiD"
                     /locus_tag="VGD55_03395"
     CDS             complement(376..1164)
                     /gene="thiD"
                     /locus_tag="VGD55_03395"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0869420.1"
                     /translation="MTPPRVLTIAGSDSGGGAGIQADLKTMLACGVHGMSVLTAVTAQ
                     NSLGVQGYWELPPEAVQAQLDSVLGDIGVDAVKTGMLASSGLVSLVSQVLRDVGVPVV
                     VDPVGVSKHGDALLAADAVGAIREELLPVATVVTPNLYEVAQLTGVKVTDEAGLREAA
                     EAVHALGPRWVLVKGGHLEGDAVDVLYDGTTQYLYRAPRLDNRHTHGTGCTLASAIAS
                     FLALGDSVPEAVQRAKDYVTGAIAGGFALGSGIGPVDHGWRWRE"
     gene            complement(1174..2100)
                     /locus_tag="VGD55_03400"
     CDS             complement(1174..2100)
                     /locus_tag="VGD55_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0869421.1"
                     /translation="MIGMREGETGPVHELALPSTTDLGLITIAVIGVSTSGPLIAATA
                     APALALAFWRNALAAGVLLPVALTRQRSELRNLSRREWGLTLAAGALLAAHFATWIPS
                     IRYTSVASSTALVASQPVWAALLARTRGVVLARSAWIGIVIAVLGAALLTGLDVRVSG
                     RALTGDLLALIGGMLAAGYVTAGAAVRRTVTTTSYTTLCYSTTAILLLVVCLVGRQSL
                     HGYSAGDWLRIAGLTVGAQLLGHSVFNRVLRTTSATVVSLSILFEVPGATIIAAIFVH
                     QHIRLEQLPAAALLLAGLALVIRSGTRSMPAE"
     gene            2126..2344
                     /locus_tag="VGD55_03405"
     CDS             2126..2344
                     /locus_tag="VGD55_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869422.1"
                     /translation="MTRDIPDPTGGWADGRSPYLWDKELGGMLRVVRGVARPDLHRRE
                     VRRAMIGLVFVVLVFVVVPIVAMLVAAN"
     gene            complement(2348..3313)
                     /locus_tag="VGD55_03410"
     CDS             complement(2348..3313)
                     /locus_tag="VGD55_03410"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF004351.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY0869423.1"
                     /translation="MSIGDLGEFGLIARVTARLPAGQHTLLGPGDDAAVVSAPDGRVV
                     ATTDLLVDGRHFRRDWSTPYDIGRKAAAQNLADIAAMGAVPTSLLVGLAAPPDLAAFW
                     AERFADGLRDECDLVEAGVVGGDVVRSDLLLIAVTALGDLQGREPVTRAGANVGDVVA
                     FAGRLGWAAAGLATLSRGFRSPRVVVDAHRRPQPPYQAGPDAARSGATSMVDISDGLV
                     ADLGHVARASAVQVNIRSSAFEIPDALSQVGSALSIDPLTWVLRGGDDHALVATFPAG
                     ATLPIGWHDIGSVVGGVVEADPGVVQGTVTVDGTPYPAGGFDHFA"
     gene            3403..3636
                     /locus_tag="VGD55_03415"
     CDS             3403..3636
                     /locus_tag="VGD55_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY0869424.1"
                     /translation="MVQAYILIQTEVGKAASVAREISGMKGVTLAEDVTGPYDVIVRA
                     EARNIDEMGRMVVAQVQSVDGITRTLTCPVVHL"
     gene            complement(3646..4404)
                     /locus_tag="VGD55_03420"
     CDS             complement(3646..4404)
                     /locus_tag="VGD55_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869425.1"
                     /translation="MSSDFDFFAGQRTPPTPAAPAAPSAWAPAAASPWAAAASTAWAP
                     PIQQDQRFGSTVGEWAPMSTAPTGQFAPAPRSSRKPLTIVAVLAVAAIACGAFYIHTR
                     PHPVPLPAALAGLPKVSLPSAQAHELSSDMHSLRSDGVHDVTFGVYGSLAGGHPAFVV
                     VAGRTRSTIPDFNQLASAVDQVGQDQGVAVTPQTLTSGASTFECATITSQGQSVPLCA
                     WQGTHAVLLGFGAQISVQDTADALEQTRLGANLS"
     gene            complement(4497..>4975)
                     /locus_tag="VGD55_03425"
     CDS             complement(4497..>4975)
                     /locus_tag="VGD55_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HEY0869426.1"
                     /translation="DAAIELARQSDPKVIVEAKIEGREIEVGVLEGIDGGPPEVSVAA
                     EITMRGSREFYDFDAKYLDLDTDITVPAVLSPDIEVQIRHFAVMAFEALSCEGLARVD
                     FFVTPSQRVLVNEVNTMPGFTPTSVFPRVWAASGIDYPALLDRLIQTALRRPAGLR"
BASE COUNT          837 a         1680 c         1695 g          763 t
ORIGIN      
        1 ggcgcttgcg tacaacgaga gcgcctctgg ttgacgcagg ccgggtctca agccgtagtc
       61 gcgttgcgcc gagcgtgcgc gcgtgtgctt cgaggttcgc caacatcgcg cgcgcgatgc
      121 cgcgacggcg ggccgccggc acgacataca tgcgcttcag ctctccgacg gaaggtgtga
      181 cgagccgcac gcccccgcat gcgacagcct ggtcgacgtc ccacccgatg acgaaaatgc
      241 cttgtggtgg cgtgaattcg tcgaccgaca gttcactgag gtcacggctg ccgtagcgca
      301 cgacgtactc ctgctggacg gcctcgatca gttcgagcgc cacgggtgag tcgagtggag
      361 ctggcctgaa ctcgatcatt cgcgccaccg ccagccatgg tcgacgggcc cgatgccgct
      421 gccgagggca aatccaccag cgatcgcgcc agtcacgtaa tccttcgcgc gctgcaccgc
      481 ctcggggacc gaatcgccga gcgcgaggaa cgacgcgatt gcactcgcga gcgtgcagcc
      541 ggtgccgtgg gtgtgccggt tgtcgaggcg cggcgcgcgg tataggtact gcgtggtccc
      601 gtcgtacagc acgtcgaccg cgtcgccctc gaggtgtccg cccttgacca gcacccatcg
      661 cgggccgagt gcgtgcacgg cctccgctgc ctcgcgcaac cccgcctcgt cggtcacctt
      721 cacgccggtc aattgcgcga cctcgtagag gttcggcgtg actaccgtcg ccaccggcag
      781 cagctcttct ctgatggcac cgacagcatc ggccgcgagt aacgcatcgc cgtgcttgga
      841 aacgccgacc ggatcgacga ccaccggaac gccgacatcg cgcaacacct gcgatacaag
      901 cgagacaagg ccggacgacg cgagcatgcc cgtcttgact gcgtcgacgc cgatgtcgcc
      961 cagcaccgaa tcgagctgtg cttgcaccgc ctcgggaggc agctcccagt agccctgcac
     1021 gccgagggag ttctgggcgg tgactgcggt gagcacgctc atgccgtgca cgccgcaggc
     1081 aagcatcgtc ttcagatcgg cttgaatgcc tgccccgcca ccggaatcgg agcccgcgat
     1141 cgtcagcacg cgcggcgggg tcatgagggc agtttattcg gcgggcatcg agcgggtgcc
     1201 cgaccggatc accagcgcga gtcctgcgag cagtaacgcc gccgccggta actgctcgag
     1261 ccggatgtgc tgatgcacga agatcgccgc gatgatcgtt gctccgggca cctcgaagag
     1321 gatcgacaga ctgacgacgg tcgcactcgt cgtccgcaag acgcggttga ataccgaatg
     1381 gccgagcaac tgcgcaccga cggtgaggcc cgcgatccgc agccagtcgc ccgcgctgta
     1441 gccgtgcagg ctctggcgtc ccacaaggca cacgaccagc agcaagatgg ccgtcgtcga
     1501 gtagcaaagc gtggtgtagc tggtcgtcgt gaccgtccgc cgcacggccg cgccggccgt
     1561 cacgtagccc gcggccagca taccgccgat gagggcgagc aggtcgccgg tgagcgcgcg
     1621 tccggatact cgcacatcca gcccggtcaa cagtgcggcg ccaagcaccg cgatcacgat
     1681 cccgatccat gccgagcgcg caagcacgac gccccgggtg cgcgcgagga gcgcggccca
     1741 aaccggctgc gatgcgacga gcgcagtaga tgacgcgacg gaggtgtacc tgatgctcgg
     1801 gatccaggtc gcaaagtggg cggcgagcag cgctcccgcg gccaacgtca ggccccactc
     1861 gcggcgcgac aggttgcgca gttcggaccg ttgccgggtc agcgcgacgg ggagcaatac
     1921 ccccgcggca agcgcgtttc gccagaacgc gagcgccagc gcgggtgctg ccgtggccgc
     1981 gatcagcggg ccggacgtcg atacgccgat cacggcaatc gtgatcagtc cgaggtcggt
     2041 ggtcgagggc aaagccagct cgtgcaccgg ccccgtctcg ccctcgcgca ttccgatcat
     2101 gatgccaccg gaccatactg gggtcgtgac gcgcgatatc cctgatccga cgggtggctg
     2161 ggccgatggc cgcagtccct acctgtggga caaggagctc ggcggcatgc tccgggtagt
     2221 tcgcggggtc gcccgccctg acttgcaccg ccgggaagtc cggcgcgcga tgatcggcct
     2281 cgtcttcgtg gtcttggtat tcgtcgtcgt gcccatcgtc gcgatgctgg tggccgccaa
     2341 ctagcggtca ggcgaagtga tcgaaaccgc ctgctggata gggagttccg tcgaccgtga
     2401 cggtgccctg aacgacaccg ggatcggcct cgaccacgcc cccgactact gagccgatgt
     2461 catgccagcc gatcggtagc gtcgctcccg ccgggaaggt cgcgacgagg gcgtggtcgt
     2521 caccaccgcg cagcacccac gtcagcgggt ctatggaaag cgccgacccg acctgactca
     2581 aggcgtcggg gatctcgaat gcgctcgacc ggatattcac ctgcaccgca ctcgctcgcg
     2641 cgacgtggcc gaggtctgcc accaacccgt cactgatgtc gaccatcgag gtcgcgccgg
     2701 acctcgcggc gtccggccct gcttgataag gcggttgcgg acgccgatgg gcgtcgacga
     2761 ctacgcgagg cgaccggaat cccctgctca gggtggcaag tcctgccgcg gcccagccga
     2821 gccgaccggc gaacgcaacg acatcgccga cgttcgcgcc cgcccgcgtg accggttcgc
     2881 gtccctgaag gtcgcctaga gcagtcaccg cgatgagaag gaggtcactg cgcacgacat
     2941 ctcccccgac caccccggcc tcgaccaggt cgcactcgtc gcgcagcccg tcggcgaatc
     3001 gctcggccca gaatgccgcc aaatcgggcg gcgcggccag cccgacgagc aacgacgtgg
     3061 gcaccgcacc catcgcggcg atgtcagcga ggttttgggc ggcggccttg cggccgatgt
     3121 cgtacggcgt cgaccagtcg cgccggaagt ggcgcccgtc gacgagcaga tcggtcgtcg
     3181 cgaccacgcg gccgtcaggc gcactcacca ccgccgcgtc gtcgccgggc ccgagcagcg
     3241 tgtgctgccc ggccgggagc cgcgccgtta cgcgcgcgat caggccgaac tcccccaggt
     3301 cgccgatgct gatctctcac ctccggactt tcgttgctac ggtgtcaccg acattgttgg
     3361 acatcctcgt atcggctgga cctcgaaggg ttgcgcgcca tggtggtaca ggcctacatc
     3421 ctgatccaga ccgaggtcgg taaagcagcc tcggtggccc gtgagatctc cggcatgaag
     3481 ggtgtcacgc tcgccgagga cgtcaccggc ccctacgacg tgatcgtgcg cgccgaggcg
     3541 cgcaatatcg atgagatggg ccgcatggtg gtcgcccagg tgcaaagcgt cgacgggatc
     3601 acccgcaccc tgacctgccc ggtcgtgcac ctgtaggcag cgcggtcagc tgaggttggc
     3661 gcccaacctc gtctgctcga gcgcgtcagc ggtgtcttgc acagagatct gcgcgccgaa
     3721 gccgaggagc acggcgtgcg tgccctgcca tgcgcacaac ggaaccgact gaccctgcga
     3781 cgtgatagtg gcgcactcga aagtcgacgc gcccgacgtc aaggtctgcg gggtgacggc
     3841 gactccttgg tcctggccga cctggtcgac cgcactggca agctggttga aatcggggat
     3901 agtcgaccgc gtacgcccgg cgacgaccac gaatgcggga tggcctccag cgagactgcc
     3961 gtagacgccg aacgtgacgt cgtgcacgcc gtcactgcgc aggctgtgca tgtcggagct
     4021 gagctcatgc gcctgcgccg aagggagcga aaccttcggc aggccggcga gcgctgccgg
     4081 caacggcacc gggtgcggac gtgtgtggat gtagaaggca ccgcaggcga tggccgcgac
     4141 tgcgaggacc gcaacgatcg tgaggggttt ccgcgacgat cgtggcgcgg gagcgaactg
     4201 tcccgtaggt gcggtcgaca tgggagccca ttcgccgact gtcgatccga aacgctggtc
     4261 ctgctgtatc ggaggcgccc atgccgtact ggccgcggct gcccacgggg acgcggccgc
     4321 gggtgcccac gccgaaggag ccgctggcgc ggccggtgtg ggcggcgtgc gctgcccggc
     4381 gaagaagtcg aagtcgctcg acacgtgcct cccgtcgaat ggcttgttga tcttcggcga
     4441 gtcgcgatcg tggcttaagg cgttgctttg gagaggccgc ggcagccgtg tgcggactag
     4501 cgcagaccgg ccggacgccg caacgcggtc tggatcaggc gatcgagcag cgcgggatag
     4561 tcgataccgc tcgccgccca cacgcgcgga aacactgagg tcggggtgaa tccgggcatc
     4621 gtgttgacct cgttgacgag cacccgctgt gagggcgtga cgaagaagtc gacgcgggca
     4681 agtccttcgc acgacagcgc ctcgaacgcc atcaccgcga agtggcggat ctggacttcg
     4741 atgtcgggcg aaagcacggc tggcacggtg atgtcggtat cgaggtcgag gtatttcgcg
     4801 tcgaagtcgt aaaactcgcg gctaccgcgc atggtgatct cggccgcgac gctgacttcg
     4861 gggggtccgc cgtcgatgcc ttcgagcacg ccgacctcga tctcacggcc ctcgatcttc
     4921 gcctcgacga tcaccttcgg atcgctctgg cgggccagct cgatcgcggc gtcga
//
LOCUS       DASWVF010000194         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_260204, whole genome shotgun sequence.
ACCESSION   DASWVF010000194 DASWVF010000000
VERSION     DASWVF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260204"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGD55_03430"
     CDS             <1..258
                     /locus_tag="VGD55_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0869427.1"
                     /translation="PRFLHSTGQYHKGGPQTGAFLQITGVVTRDLAIPGRPFGFARLQ
                     MAQALGDLRALQQRGRPAIRLHLLDRTQGLQELLNAAGDAR"
     gene            255..1793
                     /gene="zwf"
                     /locus_tag="VGD55_03435"
     CDS             255..1793
                     /gene="zwf"
                     /locus_tag="VGD55_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0869428.1"
                     /translation="MSPLVGDVHNPLRDPRDRRLPRVAGPCGLVLFGVTGDLARKKLM
                     PAIYDLANRGLLPPGFALVGFARRDWADEDFAQVVHDAVKQYARTPFREEVWNQLAEG
                     FRFVPGDFDDNNAFDALAQTINDLDRVRGTGGNHAFYLSIPPKLFPVVVGQLKRSGLS
                     AKNGDQWRRVVVEKPFGHDLKSARALSDTLNDVFPPDSVFRIDHYLGKETVQNILALR
                     FANTMFEPIWNRSYVDHVQITMAEDIGIGGRAGYYDGIGAARDVIQNHLLQLLALIAM
                     EEPVSFEAQSLRLEKTKVLSAVRIPDDLAAYTARGQYAGGWQGGEKVLGYLEEDGIPK
                     TSKTETYAAIKLEVDTRRWAGVPFYLRAGKRLGRRVTEVALEFQRAPHLPFAATATEE
                     LGQNALVIRIQPDEGVTLRFGSKVPGTAMEVRDVNMDFAYGESFTESSPEAYERLILD
                     VLLGDPPLFPRHEEVELSWEILDPIEDFWEHHGHPEQYRSGGWGPDSAIAMLARDGRV
                     WRRP"
     gene            1790..2983
                     /locus_tag="VGD55_03440"
     CDS             1790..2983
                     /locus_tag="VGD55_03440"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEY0869429.1"
                     /translation="MNRDLQNTKSSAVLNALTEARRGAGNAALGMVLTLVIDVSESEH
                     YDALQAANAAAREHPCRIVTVIRRPGKSAPRLDAEIRVGDNLLGEVIVLRLYGLLRDQ
                     ADSIVLPMLLPDAPVLVWWPGQGPAVPSLDHLGLLAQRRVTDAASSPKPTESLRERAL
                     DHSPGDTDFAWTRITPWRTLLAAALDQPFDDIVSGEVSAARNNASAELLAAWLENRLD
                     IEVTRKTSRGPGITGVQLLTRRGEISIMRPDGRLATLVRTGQPDRAVPLPRRATEELL
                     AEELRRLDPDDVYNETIHCLFGNTPGAGRRQAAAEVKPQELTKRSAPARKSAATRASE
                     VADSPAAKPQPPVAKRLPAKKAVPVKKAAPAKKAAAVKKTAPTKNALPVKKAAPAKKA
                     LRRRP"
     gene            2980..3768
                     /gene="pgl"
                     /locus_tag="VGD55_03445"
     CDS             2980..3768
                     /gene="pgl"
                     /locus_tag="VGD55_03445"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY0869430.1"
                     /translation="MSTAEVVVHRDATLLARAVAARLVTRIVDAQSARGHAHVVLTGG
                     GMGTAVLAELAASLARDAIDWAHLHVWWGDERFVPAGHPDRNDVGARAALLDVVPIPP
                     AHIHSMPTSDGPDDPHGLDPEAAAARYAAELETASRPEDHGPVPAFDVLMLGVGEDGH
                     VASLFPGMPALFDERAVVGVRGAPKPPPTRLSLTFTSIASAHEVWLITAGTAKAAAVN
                     LAISGAGPVEIPAAGAHGRQRTLWLLDEAAASQLPRSLSRIASP"
     gene            3768..4400
                     /locus_tag="VGD55_03450"
     CDS             3768..4400
                     /locus_tag="VGD55_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0869431.1"
                     /translation="MAVPQRLVPLLAQFDFGRARLANRIAGPSVDSGDGSEVEVVAMT
                     DDEYLWEPVPGCWSVRRRTGGPGSRATVLAGAGEWGRDAAPWPQPWPPPVTTIAWRLS
                     HLSEMLTLRADYTNGSHALTRDAYRSCGAVAAAVTAFEDAGRAWREALLSCDDAALDT
                     VGYSAYPYGSDSEEPFIEIAWWVNQEVLHHGAEIALLRDLYRARQGCAIT"
     gene            complement(4397..>4732)
                     /locus_tag="VGD55_03455"
     CDS             complement(4397..>4732)
                     /locus_tag="VGD55_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEY0869432.1"
                     /translation="AIRGSRVGAGPMGEAERGESAPRRRISFWCANGHETRPSFASDA
                     VIPESWDCPRCGLPAGPDRDVPPTAPRSEPYKTHLAYVRERRNDADGEAILAEALERL
                     RTSKVYPRI"
BASE COUNT          798 a         1623 c         1577 g          734 t
ORIGIN      
        1 ccccgcttcc tgcactcgac cgggcagtac cacaagggtg gaccgcagac cggcgcgttc
       61 ctccagatca ccggagtcgt cacccgtgac ctcgccatac cgggtcgccc attcgggttc
      121 gctcggttgc agatggcgca ggcacttggt gatttgcggg cgctccagca acgcgggcgg
      181 ccggcgatcc gcctgcatct gctcgaccgc acgcagggcc tccaggaact gctcaatgcc
      241 gcgggcgatg ctcgatgagc ccactcgtcg gcgacgtgca caacccgctt cgcgacccgc
      301 gcgaccggcg gctaccgagg gtcgctggtc cgtgcggtct cgtcttgttc ggagtcaccg
      361 gcgacctggc ccgcaagaag ctgatgccgg cgatctatga cctcgccaac cgcggcctgc
      421 tcccgccggg cttcgcgctc gtcgggttcg cgcggcgcga ctgggccgac gaggacttcg
      481 cccaagtcgt acacgacgcc gtgaagcaat acgcacggac gccgtttcgc gaagaggtct
      541 ggaatcagct cgccgaaggc tttcggttcg tgccgggcga cttcgacgac aacaacgcgt
      601 tcgacgcgct cgcgcagacc atcaacgacc tcgaccgggt gcgcggcacc ggcggcaacc
      661 acgcgttcta cctgtcgatc cccccgaagc tcttcccggt cgtcgtcggg cagctgaaga
      721 gatcaggatt gtcggccaag aacggcgatc agtggcgccg ggtcgtcgtc gagaagccgt
      781 tcggtcacga cctcaagagc gcgcgagcct tgagcgacac cctcaacgac gtgttcccgc
      841 ccgactcggt gtttcgcatc gaccactacc tgggcaagga gacagtccag aacatcttgg
      901 cgctgcgttt cgccaacacg atgttcgagc cgatctggaa ccgctcgtac gtcgaccacg
      961 tgcaaatcac gatggccgag gacattggca tcggcgggcg cgcgggttac tacgacggca
     1021 tcggcgccgc acgcgacgtc atccagaacc acctgctcca gttgctcgcg ctcatcgcga
     1081 tggaagaacc ggtgagcttc gaagcgcaat cgttgcgtct tgagaagacg aaggtgctct
     1141 cagcagttcg aatccccgac gaccttgcgg cctacaccgc gcgtgggcag tacgcgggcg
     1201 gctggcaggg tggggagaaa gtgctcggct acctcgagga agacggcatt cccaagacct
     1261 cgaagactga gacctacgca gccatcaagc ttgaggtcga cacgcgacgc tgggcgggcg
     1321 tccctttcta ccttcgtgca ggcaagcggc tcggacgtcg agtgacggag gtcgcgttgg
     1381 agtttcagcg ggcgccgcac cttccgtttg cggcgacggc gaccgaagag ctcggccaaa
     1441 acgcactcgt gatccgcatc cagcccgacg aaggcgtgac gctgcgattc ggctcgaaag
     1501 tgcctggtac cgcgatggaa gtgcgcgacg tgaacatgga cttcgcctac ggtgaatcgt
     1561 tcaccgagtc gagccccgaa gcctacgagc ggctcatcct cgacgtgctg ctcggcgatc
     1621 cgccgctctt cccgcgacac gaagaagtcg agctctcgtg ggaaatcctt gatcccatcg
     1681 aggatttctg ggagcaccac ggccaccccg agcaataccg atctggtggg tgggggcctg
     1741 actccgcgat cgcgatgctg gcccgcgacg gtcgcgtttg gcggcggcca tgaaccgcga
     1801 cctccagaac acgaaatcgt cggcagtcct gaatgcgctg acagaagcgc ggcgcggcgc
     1861 gggtaatgct gcgctcggca tggtgctcac gctggtgatc gacgtgagcg agagcgagca
     1921 ctacgacgcg ttgcaggctg ccaacgcagc tgcccgcgaa cacccgtgcc ggatcgtcac
     1981 ggtgatcagg cgaccaggca agtcggcccc tcgcctcgat gccgagatcc gcgtcggtga
     2041 caacctgctc ggtgaggtca tcgtgttgcg gctctacgga ttgctgcgcg accaggccga
     2101 ttcgatcgtg ctgccgatgt tgctgcccga tgcgccagtg cttgtctggt ggccagggca
     2161 ggggccggcc gttccgtcgc tcgatcacct cggcctgctc gcccaacgcc gggtgaccga
     2221 cgccgcgtcg tctccgaagc cgaccgagtc gttgcgcgaa cgagcgcttg accactcccc
     2281 tggcgacacc gacttcgcct ggacccgcat caccccgtgg cgcacgttgc tagcggccgc
     2341 cctcgaccag ccgttcgacg acatcgtctc cggcgaagtc agcgctgcgc gcaacaacgc
     2401 cagcgccgaa ctgctcgcgg cctggctcga aaaccgcctc gatatcgagg tgacccgcaa
     2461 gacgagccgc ggccccggca tcaccggcgt ccagttgctc accaggcgcg gcgagatctc
     2521 gatcatgcgc cccgatggca ggctcgccac cttggtgcgc accggtcaac cagatcgcgc
     2581 ggtgccactg cccaggcgtg ccaccgaaga gttgctcgcc gaggagttgc ggcgcctcga
     2641 ccccgacgac gtctacaacg agacgatcca ctgtcttttc ggcaacacac caggggccgg
     2701 acgccgacag gcggccgccg aggtcaagcc gcaggagtta accaagcgat cggcgccggc
     2761 gcgcaagtca gcagcgacgc gggctagtga agtcgccgac tcccccgcgg caaagccaca
     2821 gccgcccgtc gcgaagcggc tgccggccaa gaaagcggtg ccggtgaaga aagcggcccc
     2881 ggccaagaag gccgcagccg tcaagaaaac cgcgcccacc aagaacgcgc tccccgtcaa
     2941 gaaagctgcg cctgcgaaga aggcgttgcg gcgtaggccg tgagtaccgc cgaagtcgtc
     3001 gtccaccgcg atgcaacact gctcgcgcgt gcggtcgcgg ctcggctcgt cactcgcatc
     3061 gtcgatgcac agtcggcgcg cggccacgcc catgtggtgc tcacgggtgg cggcatgggc
     3121 acggcggtgc ttgccgagct ggcggcgtcc ctggcgcgag atgcgatcga ctgggcccac
     3181 ctgcacgtgt ggtggggtga cgagcgcttc gtaccagccg gccatcccga ccgcaacgac
     3241 gtcggggcgc gagcggcgct gctcgacgtc gtccccatcc cgccggcaca cattcattcg
     3301 atgccgacaa gtgatgggcc tgacgacccc cacggcctgg atcccgaggc agcggcggcc
     3361 cggtacgcgg ctgagcttga gactgcgtcg cgccccgaag accacggtcc ggtgcccgcg
     3421 ttcgacgtgc tgatgctcgg tgttggcgaa gacggccacg tggcgtcgtt gttccccggc
     3481 atgcccgcgc tgttcgacga gcgggcagtg gtcggcgtcc gcggggcacc gaaaccaccg
     3541 ccgacgcggc tgtcgctgac gttcacctcg attgcctcag cacacgaggt gtggttgatc
     3601 acggctggca cggcgaaggc agcagcggtg aatctcgcga tcagcggcgc cggcccggtc
     3661 gagatcccgg cggccggcgc acacgggcgg cagcgcacgt tgtggcttct agacgaggca
     3721 gcagcgagcc agcttccgcg cagcctgtcg cggatcgcgt cgccctagtg gcagtgccac
     3781 agcgcctcgt cccgttgctg gcgcagttcg atttcgggcg cgcccgcctc gccaaccgca
     3841 tcgccggccc gagtgtagac agcggcgacg gctccgaggt cgaggtcgtc gcgatgaccg
     3901 atgacgagta tctgtgggaa cccgtgccgg gttgttggtc ggttcggcgt cggactggag
     3961 ggcccggctc gcgagcgacc gttcttgccg gcgcgggcga gtggggacgc gatgccgccc
     4021 cgtggccaca accgtggccg cccccggtca cgaccatcgc ctggcggctc agccacctca
     4081 gcgaaatgct cacgttgcgg gctgactaca cgaacggcag ccatgccctc acgcgcgacg
     4141 cgtaccgctc gtgcggcgcc gtcgcggccg ctgtcacggc gttcgaggac gcgggccggg
     4201 cgtggcgtga agcgctgctc agttgcgatg acgcagcgct cgacacagtc ggatacagcg
     4261 cctaccccta cggcagcgac tcagaagaac cgttcatcga gatcgcctgg tgggtgaatc
     4321 aggaagtgct gcaccacggc gccgagattg ccctactgcg cgatctgtac cgcgcgcggc
     4381 agggctgcgc gatcacctag atgcgcgggt acaccttcga ggtgcgcagc cgctcgaggg
     4441 cctcggcaag gattgcctcg ccgtcagcgt cgttgcgtcg ttcgcgcacg taggcgaggt
     4501 gggtcttgta aggctcgctg cgcggagcgg tcggcggcac atcgcggtcg gggccagcag
     4561 gcaagccgca gcgtgggcaa tcccatgact ccgggatcac cgcatcggag gcgaacgaag
     4621 gacgcgtctc gtgaccgttg gcacaccaga acgagatgcg acgtcgaggc gccgactcgc
     4681 cgcgctcggc ctcgcccatc ggcccagcgc ctacccggct tccgcgaatc gc
//
LOCUS       DASWVF010000195         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_30657, whole genome shotgun sequence.
ACCESSION   DASWVF010000195 DASWVF010000000
VERSION     DASWVF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30657"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1781
                     /locus_tag="VGD55_03460"
     CDS             444..1781
                     /locus_tag="VGD55_03460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0869433.1"
                     /translation="MTAIKASLASLMSAPRGLTSLPRAARRFVLAVSALYIAAIVVGL
                     LTTRLTAADGLAFATLVGSALVSIEGSLRLVWQGPKAGRATNDLLAVWTIPVVLLLPA
                     LYGALVVVPIFAFLQLRVGKRPPIKLVFSMSAAGLANFAAASLHRAFVADPARWNVHS
                     LVGTPGALAVTITCVAVRHVINMGLVAEVMALTSSPSRLRDHLVDPDAWVGITAESCT
                     GVLVALACAASPYAVLFALAPVLVLQRTLLLAEFRDAARTDPKTGLANSSYWRDVAER
                     EIKRARGGGEPLAVLLVDIDNFKAVNDKYGHLTGDDVLRGVATALTDGLRPRDFVGRF
                     GGEEFVILLAGSDLEQARQTAERIRVHVAEVSIDDGRQEHVSVTVSVGVAAFRDSGHS
                     VLELLDAADTALYAAKHSGRNCVRVAEGARQQVLDLTPHGHRLIDLRQRESID"
BASE COUNT          303 a          617 c          649 g          361 t
ORIGIN      
        1 gctgctgttc ggtgccgagg gtccgggctt gtccgagagc gtcatgggat cggcgacgcg
       61 atcggtgcgg atccctatga gcaacggcgt cgattcgctg aatgtcgcgg cagcggcagc
      121 ggttgcctgc tacgcggtcg gtcggcttgg ttgacgtgcg gacatggggc gagccgccag
      181 gtcggtgggg ggttcgatcc ggcggctcgc ggtctcaggt ggctactacc accactgctg
      241 gtcgcgcata acgcactcca aacgtttgag gacctgtcgg cacctatcgg cacaaacttg
      301 aacacctcat caaaacactc gaaacgggta aatcgcgctc tcactgcagg gtgaatgcac
      361 agcgcgtaga gtgctcgact acagcgagtg atcgaggccg agtccctgcc gccgaccgtc
      421 gctgggggag cgcgcgccgg ggggtgaccg cgatcaaagc aagtcttgcg tcgctgatgt
      481 cagcgcccag agggctgacg tcactgccgc gcgcggcacg tcgtttcgtc ctggccgtca
      541 gcgcgctcta catcgccgcg atcgtcgtcg gcttgttgac gacgcgcctg acggcagccg
      601 atgggctcgc gttcgccaca ctcgtcggct ctgctttggt aagcatcgag ggctccctgc
      661 ggctggtttg gcaaggcccg aaggcgggcc gcgcgacgaa cgacctgctc gctgtgtgga
      721 ccattcccgt cgtcctgctg ctccccgcgc tgtatggcgc gctcgtcgtc gtcccgatat
      781 ttgcgttcct tcaactgcgt gtcggcaagc gaccgcccat caagctcgtg ttcagcatgt
      841 cggccgccgg attggcgaac ttcgcagcgg cgagcctgca tcgcgcgttc gtggccgacc
      901 cggcccgctg gaatgttcat tcgctggtcg gcacacctgg cgcgcttgca gtgaccatca
      961 cctgcgtcgc cgtgcggcac gtgatcaaca tgggcctggt cgccgaagtc atggcgctca
     1021 cgtcgagtcc gtcgcgattg cgcgaccatc tggtcgatcc cgacgcctgg gtggggatta
     1081 ccgccgaatc gtgcacgggc gtgctggtgg cattggcatg tgctgcatcg ccgtatgcgg
     1141 tgctgttcgc actcgcgccc gtgctggtgt tgcagcgaac ccttttgctc gctgaattcc
     1201 gtgacgcggc gcgcaccgat cccaagacgg gcctggcgaa ctcgagctat tggcgcgacg
     1261 ttgcggaacg cgagatcaaa cgtgcgcgtg gcgggggaga gccgctcgcg gtgctgctgg
     1321 tcgacatcga caacttcaag gcagtgaacg acaagtacgg gcacctgacc ggcgacgacg
     1381 ttctgcgtgg agtcgccacc gcgctgactg acggcctgcg accacgcgac ttcgtcggcc
     1441 ggttcggtgg ggaggagttc gtaatcctgt tggccggctc agatcttgag caggccaggc
     1501 agactgccga gcgcattcgg gtgcacgtcg cggaagtgtc gatcgacgac ggccggcaag
     1561 aacacgtgag cgtcacggtc tccgtcggcg tcgcagcgtt tcgcgacagc ggccactcgg
     1621 tactcgaatt gctcgacgcc gccgacaccg cgctctacgc ggccaaacac tcgggtcgaa
     1681 actgcgtgcg ggtcgcagaa ggcgctcgcc agcaggtgct tgacctgacg ccgcacgggc
     1741 accgcttgat cgaccttcgg caacgcgaat cgatcgacta gaccggttca ccgctcgggg
     1801 cgccgcgatc tcgggccgag aatatgctga ccgaatgaag cgtcgctcgg ctgccactgt
     1861 cagtgccggc ccgtcgaagt ccgatcgagc cctctccgat gccaggcgcg cattgttcgt
     1921 catggccggc
//
LOCUS       DASWVF010000196         6043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_209195, whole genome shotgun sequence.
ACCESSION   DASWVF010000196 DASWVF010000000
VERSION     DASWVF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209195"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VGD55_03465"
     CDS             <1..702
                     /locus_tag="VGD55_03465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0869434.1"
                     /translation="RPAVGKSTMALDVARSAAIRHGQTAVLFSLEMSKHEITMRLLSA
                     EARIPLQTMRTGQMRDDDWVKLTRRLGEMASAPLYIDDSPNMTMMEIRAKSRRLKQRN
                     ELRLVIVDYLQLMTSHSKVENRQQEVSQISRSLKLLAKELEVPVIALSQLNRGPEQRT
                     DKRPQLSDLRESGAIEQDADVVILLHREDMYERESPRAGEADLIVAKHRNGPTSTIVV
                     AFQGHYSRFVDMAAG"
     gene            complement(714..941)
                     /locus_tag="VGD55_03470"
     CDS             complement(714..941)
                     /locus_tag="VGD55_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0869435.1"
                     /translation="MATKTNARTGTRLGVDWTACDGRGLCAELLPEMVRRDDWGFPLI
                     ANDAVPASLMAHARRAVGACPTLALRLEVAP"
     gene            complement(952..2235)
                     /locus_tag="VGD55_03475"
     CDS             complement(952..2235)
                     /locus_tag="VGD55_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY0869436.1"
                     /translation="MTISTDRDRPDVMARGALIGLDPAAQLDVREHLTEHGQVPTLSS
                     REIVHLASDAGLTGRGGAGFPLWRKLEAVAKARGGPIVIANGAEGEPASAKDRWLLTH
                     RPHLVLDGLQLVAKAVGATDAYLYVTERVSAGGISSVISARRHAGLDRVAVRRGIAPD
                     RFISGEESAVVNVLGGGAAIPRSKPPRVVERGLNGRPTLVSNVETLALLALIARRGPA
                     WFRATGTNDAPGTMLFTVGGAVRQPGIVEAGQGTSIHDVLDLAGGLSRPLQAVLLGGY
                     HGSWLTAAEAAPARLSRADLAAFGASPGAGVVVALAQGSCGLAEISRVMSYLARESAG
                     QCGPCLNGLPRIAAQARSLAFQGTDHGAVTELQRLSALVVGRGACHHPDGSMRFLGSG
                     LRVFASEVELHLKGHCSRTSDDAVLPTPVTSDSRG"
     gene            complement(2232..2849)
                     /locus_tag="VGD55_03480"
     CDS             complement(2232..2849)
                     /locus_tag="VGD55_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY0869437.1"
                     /translation="MSAKAVDTALWYLGRATGVVSLALLTIVVALGIVTRSGRPLPGL
                     PRFAVSAVHRSASLLAVVFLVVHIVTLTLDPQSQLRWIDAVIPFGSHFKPLWIGLGAL
                     SLDLIVALVVTSLLRERIGRRVWRAIHWGAYALWPFAVLHTIGAGTDASRTWMIAFLV
                     ACGGAFVVLLGWRASAGFLDPADRVAGTRVPSPQTRSLAGTRGPR"
     gene            complement(2846..3826)
                     /locus_tag="VGD55_03485"
     CDS             complement(2846..3826)
                     /locus_tag="VGD55_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0869438.1"
                     /translation="MTAETLEIDAGLATRQWPAIGTTAHVVVRSRTDIDAATRIVVTE
                     LDALDRACSRFRVDSEIATLSRAGGAWVAVSGALSAILASALEVASFTDGDVDPTMGA
                     DLQNLGYDRDFGELVQLPAFAPLSMPLSMPLTYRVVRQPSWRDVELDEANRRVRVPAG
                     VVIDLGAIAKAWCADRCAGRVASELGAGVLVSLGGDIATAGIGPRDGWVVRVQDKPDS
                     TDGPSCAVVLHDGFAVATSSTVTRTWQRGASRLHHILDPATRLPAEPTWRTVTVAARS
                     CVRANAASTAAIVRGRRAPRWLGGLALAARLVSVDGVVSTIGGWPTEHAA"
     gene            complement(3823..4236)
                     /locus_tag="VGD55_03490"
     CDS             complement(3823..4236)
                     /locus_tag="VGD55_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869439.1"
                     /translation="MTNARRSAGLRRVSTATYGLVAASMTGVAVVTGVVAHQGVHHGT
                     SPIAQTDDGSSTGTASASPSANTDDALGNVTPQSPAAQSPVAQSPAVQSPVQQQPAQQ
                     QPAQQGPVQVLPGGPTPVVGAVQKPVHHHAVTSGS"
     gene            4533..5594
                     /locus_tag="VGD55_03495"
     CDS             4533..5594
                     /locus_tag="VGD55_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY0869440.1"
                     /translation="MRLIRVGAAVAAVVMVTGAARASTPGAAGKVLPAHVFAPYFQSY
                     QDGVSPAALAKASGARYVTMAFLETPGGGSCTPSWNNDPTKPVAWSTYGPDIAKIRAL
                     GGDVVPSFGGYSADDEGRELADSCTSVAKIAAAYEKVVTTYGVTRLDLDVEDNSLANI
                     AGIDRRNKAIAEVEAWAASHHRVVQFVYTVPTDSSGIEADVEHILRNAVENHARVDVV
                     NIMTFDYYDNAKHEMASDTMTAADALVSYLHTLCPTCSGDRLWRAVGVTEMIGYDDYG
                     SGGETGPPEIFTLSDAKAVTAWASHKHIAELSFWALNRDKTADPDPSAGCTLGTAGGD
                     TCSGIAQKPWSFTHIMGAF"
     gene            5749..>6043
                     /locus_tag="VGD55_03500"
     CDS             5749..>6043
                     /locus_tag="VGD55_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869441.1"
                     /translation="MKRFTKTTAGLAATAATAAALIGMSGTALAASPTPAASASPAHQ
                     GQNLAAIQQKAAADIARRLASLSVAINDVTNSTAISSGDKTTLLATLNGDVTGL"
BASE COUNT         1104 a         2087 c         1956 g          896 t
ORIGIN      
        1 agacctgctg tcggaaagtc aacgatggct ttagacgttg cgcgctcagc ggcgattcgg
       61 cacggccaga ccgccgtgtt gttctcgctg gagatgagca agcacgaaat caccatgcgg
      121 ctgttgtcag ccgaagcgcg catccccctg cagaccatgc gtaccggcca gatgcgcgac
      181 gacgactggg tgaagctcac ccggcggctc ggggagatgg cgagcgcgcc gctgtacatc
      241 gacgactccc cgaacatgac gatgatggag atccgcgcga aatcccggcg cctgaagcag
      301 cgcaacgagc tccggctggt catcgtcgac tacctccagc tgatgacctc gcactcgaag
      361 gtcgagaacc ggcagcagga agtgagtcag atctcgcgtt cgctgaagct gctggcgaag
      421 gagctcgagg ttccggttat cgcgttgtcg cagctcaacc gtggtcctga gcaacgcacg
      481 gacaaacgcc cgcagctgtc tgacctgcgt gagtcgggcg cgatcgagca ggacgccgac
      541 gtcgtcatcc tgctgcaccg cgaagacatg tacgagcgtg agtcaccgcg cgcgggtgag
      601 gccgacctga tcgtggccaa gcaccgcaac ggtccgacca gcaccatcgt ggtggcgttc
      661 cagggtcact acagccggtt cgtcgacatg gccgccggct aaccggccgc cggctacggc
      721 gcaacctcga gtctcagcgc aagggtcggg caggcaccaa ctgcacgcct ggcatgagcc
      781 atcagcgatg ccggcaccgc gtcgttcgcg atcaacggga aaccccaatc gtcgcgacgc
      841 accatctccg gcaacagctc ggcgcacagg ccgcggccgt cacatgcagt ccagtcaacg
      901 ccaaggcgag tacccgtgcg cgcgttcgtt ttcgtggcca tgacgtcctc ctcagcctcg
      961 cgaatcggaa gtgaccggag tcggcaacac cgcgtcgtcc gatgtgcgac tgcagtggcc
     1021 cttgaggtgc aactcgacct cgctggcgaa cacccgcaag ccgctgccga ggaaccgcat
     1081 gctgccgtcg gggtggtggc aggccccacg cccgacgacc aaggcgctca ggcgctgcaa
     1141 ctcagtgaca gccccgtgat cggtgccctg gaacgccagt gaccgcgctt gcgctgcgat
     1201 gcgcggcaag ccgttcaggc acggtccaca ctggcccgcc gattcgcggg cgaggtagct
     1261 cataacgcgg ctgatctcgg cgagcccgca actgccctgc gccagcgcga cgacgacccc
     1321 agcgccgggc gacgcaccga atgctgcgag gtcggctcgt gacaggcgag cgggcgcggc
     1381 ttcggcggcg gtgagccagc tgccgtgata gccgccgagc agcaccgcct gcaacggccg
     1441 tgacagccca ccggcaagat cgaggacgtc gtggattgac gttccttggc cagcctcgac
     1501 gatgccgggt tggcgaaccg cgccgcccac cgtgaacagc atcgtgcccg gagcatcgtt
     1561 cgtgccggta gcccgaaacc acgccggacc gcgccgcgcg atcagtgcca gcagcgcaag
     1621 ggtctcgacg ttgctgacca gcgtcggccg cccgttcagc ccgcgctcga ccacgcgcgg
     1681 tggcttcgag cgcggtatcg cggcgccgcc gccgagcacg ttcacgacag cagactcttc
     1741 gcccgagatg aatcgatcgg gagcaatccc gcgcctgacc gcgacgcggt cgaggccagc
     1801 atggcgccgc gccgagatca ccgacgagat gccaccagca gacacccgct cagtgacgta
     1861 gaggtacgcg tctgtagccc cgacggcttt cgcgacgagt tgcagcccat cgagcaccag
     1921 gtgcggacga tgggtaagca gccaccggtc cttggcgctt gcgggttcgc cctcggcgcc
     1981 gttcgcgatg acgatcggcc cgccacgcgc tttggcaacg gcttccagct tgcgccacaa
     2041 cggaaatccc gcgccgccgc gcccggtcaa cccggcgtcg gacgcaagat gcacgatctc
     2101 gcgcgaactg agggtcggca cctgcccgtg ctcagtcagg tgctcgcgga cgtcgagttg
     2161 cgcggcaggg tcgagcccga tcagcgcgcc tctggccatg acatcaggtc tgtcgcggtc
     2221 ggtactgatg gtcatcgtgg tcctctcgta ccggcgagcg agcgagtctg cggagacggc
     2281 actcgggtgc cggcgacgcg gtcagcgggg tcaaggaagc cagcggaggc acgccaaccg
     2341 agcaacacca cgaacgcgcc gccacatgcg acgaggaacg cgatcatcca ggttcgactc
     2401 gcatcggtgc cggccccgat cgtgtggagc actgcgaacg gccagagggc gtaggccccc
     2461 caatggatcg cgcgccatac ccggcgtccg attcgctccc gcagcaggct cgtcacgacg
     2521 agcgccacta tcaggtcgag cgacagcgct ccgagtccga tccacaacgg cttgaagtgg
     2581 ctgccgaatg ggatcaccgc gtcgatccat cgcagctggg actgcgggtc gagcgtcaac
     2641 gtcacgatgt gtacaaccag gaagacgacc gccagcaggc tggccgaccg gtggaccgca
     2701 ctgaccgcga agcgcggcaa tcccggcaac gggcggcccg aacgcgtcac gatcccaagc
     2761 gcgaccacga tggtcagcaa cgcaaggctc acgacgccgg tcgcacgtcc gagataccac
     2821 agcgcggtgt cgaccgcttt cgcgctcatg ccgcatgctc cgtaggccaa ccgccgatgg
     2881 tcgaaacgac gccgtcgacg ctgacgagtc gagccgcgag tgcgagcccg ccgagccacc
     2941 gcggtgctcg gcgcccccgc acgatcgcgg cggtgctcgc cgcattggcg cgaacgcacg
     3001 atcgagcggc gaccgtgacg gtgcgccacg tgggttcggc aggcagccga gtcgcgggat
     3061 ccaggatgtg gtgcaaccgg ctcgcaccac gctgccacgt tctcgtcacg gtgctcgagg
     3121 tagccacggc gaaaccgtcg tgcaggacga cggcacaact cggaccgtcg gtgctgtcgg
     3181 gcttgtcttg cacccgcacg acccagccat cgcgcggccc gatgcctgcg gtggcaatgt
     3241 cgccaccgag gctgacgagg acgccggctc cgagttcact ggcaactcgg cccgcgcagc
     3301 gatcagcaca ccatgccttc gcgattgcgc cgagatcgat gacgaccccg gcaggaacac
     3361 gcacgcgccg gttcgcctcg tcgagctcga cgtcgcgcca ggaaggttgc cgcacgacgc
     3421 gatatgtgag cggcatggac agtggcatgg acaaaggagc gaacgccgga agctgtacga
     3481 gctcgccaaa atccctgtcg tagcccaggt tttgcaggtc ggcacccatc gtcggatcga
     3541 cgtcgccgtc ggtgaagcta gcaacctcga gtgccgatgc gaggatcgcc gacagtgcac
     3601 cacttactgc gacccaagcg ccgcctgcac gactcagcgt cgcgatctcg gagtcgacgc
     3661 gaaatctgct gcatgcgcgg tcgagtgcgt cgagttccgt taccacaatg cgagttgccg
     3721 catcaatgtc ggtgcgcgat ctcacgacaa cgtgtgctgt cgtgccgatc gccggccact
     3781 gtctggtggc aaggcccgcg tcaatctcga gtgtctcggc ggtcatgagc cactcgtcac
     3841 tgcgtggtgg tgcacgggct tttgcaccgc tccgacgact ggcgtcggtc cgcccggtag
     3901 cacctgcaca gggccctgct gcgcgggttg ctgctgcgcg ggttgctgct ggaccggcga
     3961 ctgcacggcc ggtgactgcg caacgggcga ctgtgcggcg ggtgactggg gcgtgacgtt
     4021 gcccaacgcg tcgtcggtgt tcgccgatgg actcgccgac gctgtgccgg tgctcgaccc
     4081 gtcgtcggtc tgcgcgattg gcgaagtccc gtgatgcaca ccttgatggg ccacgacacc
     4141 ggtgacgacg gcgacacccg tcatgctcgc tgcgacgagc ccgtacgtgg cagtgctgac
     4201 acggcgcagg ccagccgacc gccgagcgtt tgtcatcaca tgcctcccgg ttcgccgttc
     4261 gggttcctgg tgattacctt cgagggccta gctgacccga ggccgagagt ccgctgaacg
     4321 atcgctgaga atcaaaactc ttcagcgcca gcgcccccgt tacccctggc agccaggatg
     4381 ggcggcgaac cttacggcgg cgtgacacgg gaccgcggcg acgtgcccat ggagcctcgg
     4441 ccgggtcaag accttgacac gccaggcggc caaagttacc gtcggcgaca cgttgtaagg
     4501 gttcctaact atgcgtctgg gggcgggcgg acatgcgact tatccgggtc ggggcggcag
     4561 tggcggcggt cgtcatggtc accggcgcag cccgcgcaag tacgccgggg gctgcgggca
     4621 aggtgttgcc cgcgcatgta ttcgccccgt acttccagtc ctaccaagac ggggtcagcc
     4681 cagccgcgct ggcgaaagca tcaggtgcgc gatacgtgac gatggcgttc ctcgagactc
     4741 ccggcggcgg ctcctgcacc ccgagctgga acaacgaccc aaccaagccg gtcgcctggt
     4801 cgacctatgg ccccgacatc gccaagatcc gcgccctcgg cggtgacgtc gtcccgtcat
     4861 ttggtggtta cagcgcagac gacgagggcc gcgagctcgc cgacagttgc acgagcgtgg
     4921 ccaagatcgc tgctgcgtac gagaaggtcg tcacgaccta cggcgtcacc cgccttgacc
     4981 tcgacgtcga agacaactca ctcgccaaca tcgccggcat cgatcgccgc aacaaggcga
     5041 tcgccgaggt cgaggcatgg gcggccagcc accaccgcgt cgtgcaattc gtctacacag
     5101 tgccgacaga ttcaagcggc atcgaggccg acgtcgagca catcttgcgc aatgcggtcg
     5161 agaatcacgc gcgcgtcgat gtcgtcaaca tcatgacgtt cgactactac gacaacgcca
     5221 agcacgagat ggcgagcgac accatgaccg ccgcagatgc gcttgtcagc tacctgcaca
     5281 ccttgtgccc gacgtgctcg ggggaccggc tctggcgggc ggtcggcgtc accgaaatga
     5341 tcggctacga cgactacggc tccggcggag agactggtcc acccgagatc ttcacgttgt
     5401 cggacgccaa ggccgtcact gcctgggcct cccacaagca catcgccgag ctgtcgttct
     5461 gggccctgaa ccgcgacaag acggccgacc cagacccgag cgccggctgc acactcggca
     5521 ccgcgggagg cgacacctgc tcgggcatcg cgcagaagcc atggagcttc actcacatca
     5581 tgggcgcctt ctagcgctcc cgcccgctcc agcactgggc gcgcgttcca ccgcgcttac
     5641 aaagcccgta cacagtgccg acctgtcagt tgacgtccag ggccgacaaa ggaggtacac
     5701 acaaacagcg cccgaccctc gggccgccct cgacccaagg agcacaccat gaagcgcttc
     5761 accaaaacga cagcggggtt ggcagccacg gcggccacgg ccgcagcact catcggtatg
     5821 tcaggcaccg cgttggcggc cagccccaca ccagccgcga gcgcctctcc cgcccatcag
     5881 ggacagaatc ttgcagcgat ccagcaaaag gccgccgccg acatcgcgcg ccggctcgca
     5941 tcgctcagcg tcgcgatcaa cgacgtcacg aacagcacgg ccatctcgtc gggcgacaag
     6001 accacgttgc tcgccacgct gaacggcgac gtcaccgggt tga
//
LOCUS       DASWVF010000197         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_234663, whole genome shotgun sequence.
ACCESSION   DASWVF010000197 DASWVF010000000
VERSION     DASWVF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234663"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..238
                     /locus_tag="VGD55_03505"
     CDS             29..238
                     /locus_tag="VGD55_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869442.1"
                     /translation="MIGILVKAVVKLRGRYTVSSIDSDRGDVPGWVMITLMTAVIVVL
                     IWGIASTQLTDLLNNAFGRVTKQAG"
     gene            268..657
                     /locus_tag="VGD55_03510"
     CDS             268..657
                     /locus_tag="VGD55_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0869443.1"
                     /translation="MVDFVLVSLILIPLFLAILQLGFALYVRNTLAACAQDGARYAAD
                     ENIVIQGDSAMIDSATQQAMTCVKESLPDSFGGGITATVPDITDSAGVPVLVVEVRIA
                     APFPLVGLFGIGPQVVHAQGDAMQEQP"
     gene            654..1163
                     /locus_tag="VGD55_03515"
     CDS             654..1163
                     /locus_tag="VGD55_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869444.1"
                     /translation="MNLRIRVAAVAFGRWRQLGVRPRISDDGNAVLEFVILTAFLMIP
                     LVYVIIAVLQVQGSAHGVTEASREAGRAFVEAKTSADAFRQACTAATVALRNNAGTAF
                     DCASELHVSCVSPTPCSTDLTPGQTIRVEIDINVGLEFLPTAVFGQPLTVRLHAVHDE
                     VVDEYRATR"
     gene            1160..1666
                     /locus_tag="VGD55_03520"
     CDS             1160..1666
                     /locus_tag="VGD55_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEY0869445.1"
                     /translation="MKVVDAEAGDEGQVMLLMVFFAVTVAALITAVVDVSTVFLAQRE
                     MQSTADGAALAATQQPDLAVVYTSELGPRLPLSRTKTINVLDSYAVDPARIPHECSAS
                     SYQVVGGLDADGNQRSGLQADGATVRVELTCKVPLPFLGLVADAFTGGVTIHQVASAR
                     SAVTPLAP"
     gene            complement(1762..>2321)
                     /locus_tag="VGD55_03525"
     CDS             complement(1762..>2321)
                     /locus_tag="VGD55_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869446.1"
                     /translation="YAGSDRFATALAVATALGNPTTVLLATGINFPDALAAGPAAAHV
                     GGVVLLTNGTSLTASVQAYLTAHPGKVYSVGGPAVTAYPAGTPLTGSDRYGTATAVAA
                     ALYTGPTNVGVASGVTFPDALSGGAFQAHFGGPILLSDPGVLPSPTSTYLTGSTSTIV
                     NTTIFGGTAALSNAVQTQIGTALGL"
BASE COUNT          416 a          697 c          761 g          447 t
ORIGIN      
        1 tcaaaggagc acgtagatga ttcaccgagt gatcggcatt cttgtcaaag ccgtcgtcaa
       61 gttgcgtggc aggtacacgg tttcctcgat cgacagcgat cgcggcgacg taccgggctg
      121 ggtcatgatc acactcatga cggcggtcat tgtcgtgctg atctggggta tcgcgagtac
      181 ccagctgacg gatctcctca acaacgcatt cggaagagtc accaagcaag ctggctaagg
      241 gtcggcgagt gcgacgaagg ctcggcgatc gtagacttcg tactcgtctc ccttattctc
      301 attccgctgt tcttggcaat cttgcaactc ggattcgcgc tgtatgtgcg aaacaccttg
      361 gccgcgtgcg cgcaagacgg cgcaaggtac gcggccgacg agaacatcgt gatacagggc
      421 gacagtgcca tgatcgattc cgctactcag caagccatga cctgcgttaa ggagtcccta
      481 ccggattcgt tcggcggcgg tatcacggcg acggttcctg acatcaccga ctctgcggga
      541 gtccccgttc tcgtcgttga ggtccgcatc gcagcgccct tcccactggt gggccttttc
      601 gggatcgggc cccaagtcgt acatgcccag ggcgatgcca tgcaggagca gccgtgaacc
      661 tacgaatccg ggtggcggcg gttgctttcg gaaggtggcg gcaactcggc gtccggcctc
      721 gtatttctga tgacggcaac gcggtgctgg agtttgtgat tctgacggcc tttctgatga
      781 tcccactggt gtacgtcatc atcgcggtgc tgcaggtgca gggttcggcc cacggagtga
      841 cagaggcgtc gcgggaagct ggtcgcgcct ttgtcgaggc gaagacgtcc gcagacgcgt
      901 ttcggcaggc ttgtaccgcc gcgacagtcg cgcttcgcaa caatgcggga acggcattcg
      961 attgtgcgag cgaattgcac gtgtcctgcg tgtctccgac tccgtgctca acggatctga
     1021 cgccaggcca aaccattcgc gtcgaaatcg acatcaacgt cgggctcgag tttctcccga
     1081 ccgcagtctt cgggcagccg ctgaccgtac gtctgcatgc cgttcacgac gaggtcgtcg
     1141 atgagtatcg agcgacgcga tgaaggtcgt tgatgccgag gcgggtgacg aaggccaagt
     1201 gatgttgctg atggtcttct tcgccgtgac agtcgcggct ctgatcacgg cagtcgtcga
     1261 tgtgtccaca gtgttcttgg ctcagcggga aatgcagtcg acggccgacg gcgcagcact
     1321 cgcggccacg cagcagcccg acttggcagt ggtatacacg agcgaactcg gcccccggct
     1381 tccgctctcg cgcaccaaga cgattaacgt cctcgactcg tacgccgtcg atcccgctcg
     1441 gattccgcac gagtgctctg cgtcgagcta ccaagtcgtc ggagggctgg acgccgacgg
     1501 caaccagcga tcgggacttc aggccgatgg agcaacagtg cgcgtcgagc tcacctgcaa
     1561 ggtgccgcta ccgtttctcg gcttggttgc cgacgcattc acgggcggcg tcacgattca
     1621 ccaagttgcg tccgcgcggt cagcagtgac ccctctcgcg ccgtgacgtg aaacgagacg
     1681 ggcggccgta gccacccgtc tcgtttcacg ttgtggtgca tggctgcctc gccgccgaaa
     1741 cgacgagaca gccgcttcac atcacaggcc gagcgcggtg ccgatctgcg tctgcactgc
     1801 gttcgacaga gctgcggtgc caccgaagat cgtcgtgttg acgatcgtcg aggtcgagcc
     1861 ggtcaggtac gtgctcgtcg gcgagggcag gacgcccggg tcggagagca ggatcgggcc
     1921 accgaagtgc gcctggaagg caccgccgct gagtgcgtca gggaacgtga cgccactcgc
     1981 gacaccgacg ttcgtcggtc cggtataaag cgccgcagct actgctgtcg cggtgccgta
     2041 acggtcggag ccggtgagcg gggtgccggc cggataggcc gtgactgccg ggccgccgac
     2101 ggagtagacc ttgcccgggt gggcggtgag gtaggcctgc acgcttgctg tcaatgacgt
     2161 tccgttcgtc agcagcacaa cgccaccgac atgagctgca gccgggccgg ctgcgagagc
     2221 gtcggggaag ttgatccctg ttgccaacag gacggtcgtc gggttaccga gtgcggtagc
     2281 gaccgcaagt gcggtagcaa aacggtcgga gccggcgtaa c
//
LOCUS       DASWVF010000198         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_30714, whole genome shotgun sequence.
ACCESSION   DASWVF010000198 DASWVF010000000
VERSION     DASWVF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30714"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGD55_03530"
     CDS             complement(<1..424)
                     /locus_tag="VGD55_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869447.1"
                     /translation="MSTAQEDDLGQARLAKAAEDYCNWGRWGDDDQAGTLNFITADDV
                     RAAAATIERGKVFSLAIPFDNKGPQTGELLRFNPMRFMLRDGDDTYARSLVGLPRGIG
                     GADDVIMLSTHGATHWDALAHVHYDSKMWNGYDCREVSS"
     gene            complement(435..1664)
                     /locus_tag="VGD55_03535"
     CDS             complement(435..1664)
                     /locus_tag="VGD55_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869448.1"
                     /translation="MTELADWVESCHGPLRPLAYHPLEGRPGFKMALTVVDDRWYLYL
                     GHLWHSGWTILDVTDPAAPEQVAFLPGPPGAWTLQVTIRDNLLATSLEPVPGRWGGDT
                     DGEFDEGVYIWDISTPTAPERLSHYKTGHHGTHRNKFDENGLLHLAARTAEHEGMILV
                     LLDVSDPTAPREVGRFSMGKQAPGETDSEGVLRFGLHGPSLRVDDIAYLPYGDSGLVV
                     LDVADSSNPQLLGHLSVRPPLGSQVAAHSAVPLPDRNLLILNSEAIAENCEELPQYAA
                     MVDISDPRHPRMISLFPAPTPEPESGFTSFCQKGGRFGPHNQHIGHGDPNLFNDDQVC
                     FLTYFNAGLRVYDTRDPHDVREVASLIPKSPTERIGMLPRTLTLQAEDVLVDRRGVVY
                     MTEKNSGLYIAQWQGLS"
BASE COUNT          309 a          551 c          599 g          333 t
ORIGIN      
        1 acgaactgac ctcgcggcag tcgtagccgt tccacatctt cgagtcgtag tgcacgtgcg
       61 cgagcgcgtc ccagtgcgtg gcaccgtgcg tcgacagcat gatcacgtcg tcggcgccgc
      121 cgatcccgcg cggcaacccg acgagcgaac gcgcatacgt gtcgtcgccg tcgcgcagca
      181 tgaagcgcat cggattgaag cgaagcagct cgccggtctg cggacctttg ttgtcgaacg
      241 ggatcgcgag cgagaagacc ttgcctcgtt cgatggtcgc cgccgcggcg cggacgtcgt
      301 cggcagtgat gaagttcaac gtgccggcct ggtcgtcgtc accccatcgg ccccagttgc
      361 aatagtcttc ggccgccttc gcgagacgtg cttgcccgag gtcatcttct tgcgcggtac
      421 tcacgtgggt ctccttagga aaggccttgc cattgcgcga tataaaggcc gctgttcttc
      481 tctgtcatgt agaccacccc gcggcggtcg accagcacgt cttcggcttg cagtgtcagc
      541 gtgcgtggca gcatgccgat gcgctcggtg ggggatttcg gtatcaagga agcaacttcg
      601 cggacgtcgt gtggatcccg cgtgtcgtag acccgaaggc ctgcattgaa gtaggtcaga
      661 aaacacacct ggtcgtcgtt gaacaggttc gggtcgccgt gcccgatgtg ctggttgtgc
      721 ggaccgaagc ggccgccctt ctggcaaaac gaggtgaacc cagactcagg ttcaggcgtc
      781 ggtgctggaa acaggctgat catgcgcggg tgccgcggat cgctgatgtc gaccatggcg
      841 gcgtactgcg gcagttcttc gcagttctcg gcgatcgcct cggagttcaa gatcagcagg
      901 ttgcgatcgg gcagcggcac ggccgagtgc gcggcgacct gactgccgag tggcggccga
      961 accgagagat gcccgagcaa ctgtgggttc gacgaatcgg caacgtcgag cacgaccaag
     1021 ccggagtcgc cgtacggtaa gtaggcgatg tcgtcgacgc gcaacgaagg gccgtgcagt
     1081 ccgaaccgca gcaccccctc ggagtcggtt tcgccgggag cctgcttacc catgctgaac
     1141 cggccaacct cgcgcggtgc ggtcggatca gagacgtcga gaagaaccag gatcatgcct
     1201 tcgtgctcgg cggtgcgggc cgcgaggtgc aacagaccgt tctcatcgaa cttgttgcgg
     1261 tgcgttccat ggtggccggt cttgtaatga gacagccgct caggcgcagt cggcgtcgag
     1321 atatcccaga tgtagacgcc ctcgtcgaac tccccgtcgg tatcgccacc ccaccggccg
     1381 ggcaccggct cgagcgacgt cgcgagcagg ttgtcgcgaa tcgtcacctg cagcgtccac
     1441 gcacctggcg gcccgggtaa gaatgcgact tgctcgggtg cggctggatc cgtgacgtcg
     1501 aggatcgtcc agccgctgtg ccacaagtgg ccgaggtaga ggtaccagcg gtcgtcaacg
     1561 acggtcagcg ccattttgaa gccgggtcga ccctcgagcg ggtggtaggc gagcgggcga
     1621 agcggtccgt gacagctctc gacccagtcg gcgagctcgg tcacccgtta agtttcccgt
     1681 cgagcaactt ggcgactacc gtgcgcgtgc gctcgaggtg ctcgtacatg agttcgccgg
     1741 caagttcgac gttgccttcg atgcacgcgt cggcgatagc gcgatgctcc gc
//
LOCUS       DASWVF010000199         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_260281, whole genome shotgun sequence.
ACCESSION   DASWVF010000199 DASWVF010000000
VERSION     DASWVF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260281"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGD55_03540"
     CDS             complement(<1..221)
                     /locus_tag="VGD55_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869449.1"
                     /translation="MKLVDASSSVAAAVVAPSSVDGAPGVLSLDDVGARIVDLSGRLS
                     ATTCAWLLLIADFDTRQGYLSFGLASTAQ"
     gene            complement(348..1382)
                     /locus_tag="VGD55_03545"
     CDS             complement(348..1382)
                     /locus_tag="VGD55_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869450.1"
                     /translation="MDLAIEQVTTRSTADRWQRLSQICSDADYFELPTDPPQAVYEHI
                     ESARTDERYELWLGSVAGEPVVLGQLRLPLLDNVSNAIVEVATHPAFRRRGYATAMLE
                     RITERAQANDRSRLIGEICEPLLAVSTEIPGVAFATAAGAKPVTSEVRRVLRIAELDN
                     DQLARLHDDAVAHSQGYSLVHWDGPAPAELVDDLAILHSRMSIDAPLEEMDWEPEQWS
                     AERYRELEKRIVVSGQQRFSTAARHDASGQIVALTDIGVAHALPDSSYQWATIVLPEH
                     RGHRLGMRVKLANLEFLRRSRPRVTIVNTWNAAINDHMVSINEAIGFRAVERWREWQL
                     ELPSQSPTFS"
     gene            1472..2263
                     /locus_tag="VGD55_03550"
     CDS             1472..2263
                     /locus_tag="VGD55_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY0869451.1"
                     /translation="MQSRDDVPHLAPTAAEQAHGQVRVEPGQKRVRAYLGGGLVVDTL
                     KPLLVWERPFYPAYYLPLADVQAKLVPTGATEHSPSRGNAQIYDVVVGDAVAPSAALR
                     YLDSPFVELRDHVRFVWDSLDEWFEEDELVYTHPRDPYTRVDILHSSRHVRIEIDGVT
                     VAESRQPTMLFETRLPPRFYLPLTDVRVDLLRPSMGETHCPYKGMATYWSIDIAGKIY
                     PDYVWIYRSPFPESQKIAGLVAFYNEKVDIYLDGELQVRPHTHFS"
     gene            complement(2280..2474)
                     /locus_tag="VGD55_03555"
     CDS             complement(2280..2474)
                     /locus_tag="VGD55_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869452.1"
                     /translation="MGDHTSRRLAADGTRVGALRRPLRRRHGDRVAHYLGNMPEVGFG
                     SLVPSEVPSLGPFSSPPDEH"
     gene            2520..3026
                     /locus_tag="VGD55_03560"
     CDS             2520..3026
                     /locus_tag="VGD55_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869453.1"
                     /translation="MRSIAVAALPPAGTKMRVTCTTNPCVTVAYWIVVFRPGVAMLGQ
                     PIIEQGCEPVLRKTIATVATPIPTSGGPSHGSVICKSFSTQADTAGDAVVSDGVTIGA
                     TAMGDGEAVTFEVRKTPQPARAINPPTQLKGRNARRASTVVDTGSAYHDSVTTRPVHA
                     LGTDSLAI"
     gene            3134..>4062
                     /locus_tag="VGD55_03565"
     CDS             3134..>4062
                     /locus_tag="VGD55_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869454.1"
                     /translation="MAAVAPETRAPALPAIRLRGLRKVFGSVVAVDHVDLDVSDGEFL
                     TLLGPSGSGKTTVLRMIAGFELPTAGRVELGGVDVTNRPPFDRDVNTVFQDYALFPHM
                     SVQDNVEYGLRVRGVDRAQRRRRAGEALESVRLSGYGERRPSELSGGQRQRVALARAL
                     VNKPKVLLLDEPLGALDLKLREQMQVELKGIQREVGITFVFVTHDQQEALTMSDRIAV
                     FHAGRLEQVGPPAEVYERPQTEFVAGFVGTSNLLRGEAAQAVLGRDGVWSVRPEKIRV
                     LDPAAAVDAGERTAAGVVREVVYVGDVTRFVVEL"
BASE COUNT          718 a         1311 c         1317 g          716 t
ORIGIN      
        1 cactgcgctg tcgatgctaa cccgaacgac agataaccct ggcgtgtgtc gaagtcggcg
       61 atcagcagca accatgcgca tgtggtcgct gacagtcgcc ccgacagatc cacgatgcgc
      121 gcacccacat catcgagcga caagacacct ggcgcaccgt cgaccgaaga aggggcaaca
      181 acagcggcag caacagaaga agacgcatcg acgagtttca tgcccgcacg ctacccacac
      241 ccaccgacac ttccgggcgg acccattccg cagtgccacg ttcacgtgaa cgcgatgccc
      301 gcgatacccc gaccgcgttc acgtgcacgc gatgcggccc gctgcgttca gctgaacgtg
      361 ggagattgcg aagggagctc gagctgccat tcgcgccagc gctcgactgc gcggaatccg
      421 atcgcctcgt tgatcgacac catgtggtcg ttgatggccg cgttccaggt gttcactatt
      481 gtcacgcgcg gtctgctgcg tcgcaggaac tccaagttgg cgagtttcac ccgcatccct
      541 aaacgatgtc cgcgatgctc cggaagcacg atcgtcgccc actggtaact gctgtcaggc
      601 agcgcatgag cgacgccgat atcagtcagc gcgacgatct gacctgacgc gtcgtggcgt
      661 gctgctgttg aaaaccgctg ctgcccactg acgacgatgc gtttttccag ctcccgatac
      721 cgctccgcac tccactgctc cggctcccag tccatctcct caagaggcgc atcgatcgac
      781 atccgcgaat gcaagatcgc gaggtcgtcg acgagttctg cgggcgccgg gccatcccaa
      841 tgcacgagtg agtagccctg cgagtgcgcg acggcgtcgt catgcaaccg cgccaactga
      901 tcgttgtcca gctcggcgat tcgaagcact cgcctgacct ccgatgtcac cggcttcgca
      961 ccggccgccg tggcaaacgc gacgcctgga atttcggtcg aaacagctaa gagcggctcg
     1021 cagatttctc cgatcaggcg gctgcggtcg ttggcttgcg ctcgctcggt gatgcgctcg
     1081 agcatcgcgg tggcataccc gcggcggcgg aacgcgggat gggtggcgac ctcgacgatc
     1141 gcgttcgaca cgttgtcgag cagaggcaga cggagctggc caaggacgac tggctcgcca
     1201 gccacgctac cgagccacag ttcgtagcgc tcgtcggtgc gagcagactc gatgtgctca
     1261 tagaccgctt gcggcggatc ggtcggcaac tcgaaatagt cagcatccga acagatctgc
     1321 gaaagccgtt gccatcgatc cgctgtactg cgggtcgtca cttgttcgat tgcgagatcc
     1381 acggtctgtc tctagcagaa ggttcccgtg gcacgcgagc gagttttcgg cgtccgggaa
     1441 tcagccgtgc cagtttcgta ttgtggcagg tatgcagagt cgagatgacg taccgcatct
     1501 ggcgccgacc gcggccgagc aggcgcacgg ccaggtcagg gtggagccgg gccagaagcg
     1561 ggtgcgtgcc tacctcggcg gcggactggt cgtcgacacg ctgaagccgt tgctggtgtg
     1621 ggagcgcccg ttctatccgg cctattatct gccgctcgcg gacgtgcaag cgaagctcgt
     1681 gccgacgggc gcgacggaac attcgccgag tcgcggcaac gcacagatct acgacgtggt
     1741 cgtgggcgac gccgttgcac cctcagcggc cttgcgctac ctcgactctc cgttcgtcga
     1801 actgcgcgac catgtgcgct tcgtctggga ctcactcgat gagtggttcg aggaggacga
     1861 actcgtctac acccacccgc gcgacccgta cacgcgcgtc gacatcctgc acagttcgcg
     1921 gcatgtgcgc atcgagatcg acggcgtcac cgtagccgag tcccgccagc cgaccatgtt
     1981 gttcgaaacc aggttgccgc cgcgtttcta cctgccactg accgacgtcc gcgtagatct
     2041 tctccggcct tctatgggcg agacgcactg tccctacaag gggatggcga cgtactggtc
     2101 gatcgacatc gccggcaaga tctaccccga ctacgtctgg atttaccggt ctccttttcc
     2161 cgagagccag aagatcgcgg gactcgtcgc gttctacaac gagaaggtcg acatctacct
     2221 cgacggcgag ttgcaagtgc gcccgcacac gcatttcagt taacccgcgg gacggcgggt
     2281 tagtgctcgt ccggtgggct gctgaacggt ccgagcgacg gcacctcgct cggcacgagt
     2341 gagccgaacc cgacttcggg catgttgccg aggtagtggg cgacacgatc accgtgccgc
     2401 cggcgcaggg gccgtcgaag cgcacccacg cgagttccat cggctgcgag ccgccgggag
     2461 gtgtgatcgc ccacgagccg ggcttgccac attcgtcatt ggagcctccg ggttgcgcga
     2521 tgagatcgat cgccgtcgcg gcgctgcccc cagcgggcac gaagatgcga gtgacctgca
     2581 ccacgaatcc ttgcgtgacg gttgcgtatt ggatcgtggt cttcaggcca ggtgtcgcga
     2641 tgctcggcca gccgatcatc gagcagggct gcgaacctgt attgcggaag acgatcgcga
     2701 cggtcgcgac tcccatcccg acgtcgggtg gtccgtccca cggatcggtg atctgcaagt
     2761 ccttctccac gcaggccgac acggcaggtg atgcggtggt ttccgacggc gtgacaattg
     2821 gcgccacggc aatgggggac ggcgaggcgg tgacgttcga ggtccggaaa acgccgcagc
     2881 cggccagggc gatcaaccca cccacgcagc tcaaaggcag gaacgcgcgc cgggcgagca
     2941 ccgtcgttga cacgggaagt gcgtatcacg attctgtgac gacccggccc gtccacgcgc
     3001 tgggaacgga ttcgctcgcc atctgagctc tccccgcctg aattcgccgc ttcgagtgca
     3061 cacgcgcaac ggattccgcg ctatcgtgcc agcctgatcg cggtcgtgaa cgtagagact
     3121 ctgcgaggtg cgcatggcgg cggtggcgcc cgagacgcgc gcccctgcgc tcccggccat
     3181 ccgcctgcgc gggctccgca aggtgttcgg atccgtcgtc gcggtcgacc atgtcgatct
     3241 tgacgtcagc gacggcgagt tcctgaccct gctcggcccg tcggggtctg gaaagaccac
     3301 tgtgctccgc atgatcgccg gcttcgaact tccgacggcg ggccgcgtcg aactgggcgg
     3361 ggtcgatgtc acgaaccggc caccgtttga tcgcgacgtc aacaccgtct ttcaggacta
     3421 cgcgctcttc ccgcacatgt cggtgcaaga caacgtcgag tacgggcttc gggtgcgcgg
     3481 cgtcgatcgc gcgcaacgcc gccggcgggc cggcgaggca ctcgaaagcg tccggctgag
     3541 cgggtacggc gagcggcgtc cgagcgagct ttctggtggg cagcggcagc gagttgccct
     3601 ggcccgagca ctcgtcaaca agccgaaggt gctgctgctc gacgagccgc tgggcgcact
     3661 cgacttgaag ctgcgcgaac agatgcaggt cgagctcaaa ggcattcaac gtgaggtcgg
     3721 catcaccttc gttttcgtga cgcacgacca gcaggaagcc ctcacgatgt ccgaccgcat
     3781 cgcggttttc catgcgggcc gcctcgaaca ggtcgggcca ccagctgagg tctatgagcg
     3841 cccgcaaaca gagttcgtcg ccgggttcgt cggcacatcg aacctgttgc gcggtgaggc
     3901 ggcacaagct gtgctcgggc gcgacggggt gtggagtgtg cgtccggaga agatccgcgt
     3961 gctcgacccg gcggcagcag tcgatgcagg cgagcggacg gcggcgggtg tggtccgcga
     4021 ggtggtgtat gtcggcgacg tcactcggtt cgtggtcgag ct
//
LOCUS       DASWVF010000200         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_158161, whole genome shotgun sequence.
ACCESSION   DASWVF010000200 DASWVF010000000
VERSION     DASWVF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158161"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGD55_03570"
     CDS             <1..902
                     /locus_tag="VGD55_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869455.1"
                     /translation="HCRDHATARRGAPPVTSGPAGAMEAIVTLRFFGQVVIVDTREPS
                     LADVVRALWRPFVSPADGSADAAAASPPVHLAVPHGGTRAEDLGWLNSTLNSTAMELT
                     PHLAVHAGVVRRGDIVVAFPAHSGVGKSTLTGACLRAGFEYVSDESLCLSYENNWVHP
                     YPRPIGLSAWSAQALDVDGVRAGDDVLVLPQDFGSTSTAIDESLRLTHVVLPRRIDKS
                     AEPSSLAAAPAHEAIEALLRMSFNHYRRPVDAMRLVASVVADADLSVLTYSDARDAAA
                     LMAQAFPEVSAGGSPLVDELPHP"
     gene            complement(870..1706)
                     /locus_tag="VGD55_03575"
     CDS             complement(870..1706)
                     /locus_tag="VGD55_03575"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEY0869456.1"
                     /translation="MSDNVPTDPAAAATASSAATTASRAAVEPQAPELLAYRLVAPTA
                     DEDLWAAPPTRVWMEQSPNRFANRCLPLLIANQSGWLLINTDTVELTWNGGAATSDIE
                     IAYPADVPPLPAISHFGMGIVTLNLPYFFRTSPGWNLLVRGPANCPKDGIYALDGVVE
                     ADWTAATFTMNWQLTRPGLPVVFERGEPLCMLVPQRRHDIENIRPAFRSLEAEPELER
                     RVRGWATSREEFLVDLDADGSQARTRRWQKDYFQGHDVDGTPAAEVHQTKLRVREFVD
                     ER"
     gene            1956..>2609
                     /locus_tag="VGD55_03580"
     CDS             1956..>2609
                     /locus_tag="VGD55_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869457.1"
                     /translation="MDVWGAITVLVRRYYLTVPIIAIALVLGWVYANKTPAEYHASAA
                     VVVIGPAAAVSKDVPQPVNPYTTIGTATVVATIQIDAGSEQSLQQLQRSGSSTNFTVA
                     ENGKTPIIDITATSRSPQQALATTNQLISIIQADLQTRQHPYAPNKANQITAPVLSPS
                     VLGALDKSAKKKALTIAIGVGIVFGIIVTLIIDGVLRARQRGREAKTDWEGPVLDREP
                     "
BASE COUNT          455 a          857 c          835 g          462 t
ORIGIN      
        1 gtcactgccg cgatcacgcg actgcacgac gaggcgctcc tccagtgacg tcaggccctg
       61 ccggagccat ggaagcaatt gtcacgctgc gcttctttgg acaggtcgtg atcgtcgaca
      121 cccgcgagcc gtcgttggcc gacgtcgtcc gggccctgtg gcgtcccttt gtatcgccag
      181 ccgatggttc cgccgacgcg gctgcggcat cacctccggt ccacctcgcc gttccccatg
      241 gcggcacccg cgccgaggat ctcgggtggt tgaacagcac cctcaacagc acggcgatgg
      301 aactgacccc ccatctcgcc gtgcacgccg gagttgtgcg acgaggcgac atcgtcgtgg
      361 cattcccggc gcattcagga gttggcaaga gcacgttgac aggcgcgtgc ctgcgcgcgg
      421 gctttgagta cgtatccgac gagtcacttt gccttagcta tgaaaacaat tgggtgcatc
      481 cttacccgcg tccaatcggt ctgtctgcgt ggtcggcgca ggcactcgac gtcgatggag
      541 tgcgtgccgg cgacgacgtt ctcgtgctac cccaagactt cggttcgacc tcgaccgcga
      601 ttgacgagtc gcttcgcctg acgcacgtcg tgttgccgcg gcgcatcgac aagagcgcgg
      661 agccctcatc actcgcagcg gcgccagcgc acgaagcgat cgaggctctc ctgcgcatgt
      721 cgttcaacca ctaccggcgg cccgtcgacg ccatgcgcct tgtcgcatcg gttgtcgccg
      781 acgccgacct cagcgtgttg acgtacagcg acgcgagaga tgcagcggcc ctgatggcac
      841 aggcatttcc ggaagtgtcg gcgggcggct caccgctcgt cgacgaactc ccgcacccgt
      901 agcttcgtct ggtgaacctc cgcagccggg gtgccgtcga catcgtggcc ctggaagtaa
      961 tccttctgcc aacgcctggt ccgggcctgc gaaccgtccg cgtcgagatc gacgaggaac
     1021 tcttcgcgcg aggtcgccca ccctcgcacc cgccgctcga gttcgggctc agcttcgagc
     1081 gagcgaaaag ccgggcggat gttctcgatg tcgtgccgac gctgcggcac cagcatgcac
     1141 agcggctcac ctcgctcgaa caccacgggc aggcccggcc tggtgagctg ccagttcatg
     1201 gtgaaggtcg cggcggtcca gtcggcctcg acaacgccgt cgagcgcgta gatgccgtcc
     1261 ttcgggcagt tcgccggccc gcgcacgaga agattccagc ccggcgacgt ccgaaaaaag
     1321 tacggcaggt tcaaggtgac gatgcccatc ccgaaatggc tgatcgccgg gagcggcggc
     1381 acgtccgcgg ggtaggcgat ctcgatatcg gacgtcgcag cgccgccgtt ccaggtcagc
     1441 tcgacggtgt cggtgttgat caacaaccag cctgactggt tggcgatcag cagcgggagg
     1501 cagcggttcg cgaagcgatt gggtgactgc tccatccaga ccctggtcgg cggggccgcc
     1561 caaaggtcct cgtcggcggt cggcgcgacc agtcggtagg cgagtaactc aggcgcctgc
     1621 ggctccacgg cggcccgaga cgctgtagtg gcagcgctgg acgcggttgc ggcagccgcc
     1681 ggatcggtgg gaacgttgtc gctcacatgc acctcctgcg tcaccggccg tcacgagcac
     1741 gtccgacagg tcatggcatc cgctgtggcc ggagagtacg acagaatcct tacaaaatcc
     1801 ttgcgccgga ggatgatttc ggtcggcgaa tggccggtcg agcccagccg gacggaaatt
     1861 ctggaacggc tgtgggcagc tgattcggcg tataccattg gcgtttcggg ggagtcgtcc
     1921 ctcatggtca ttcatctcaa ggtaggggaa ttcctgtgga cgtctgggga gccattacgg
     1981 ttctcgtacg ccgctattac ctgacagtgc cgatcattgc gatcgcactc gtgctcgggt
     2041 gggtgtacgc gaacaagacc cccgcggagt accacgcgtc ggctgcagtc gtcgtgatcg
     2101 gtcccgccgc cgcggtctcc aaggatgtgc cgcagccagt taacccttac accacgatcg
     2161 gcaccgcgac tgtcgtcgcg acgatccaga tcgacgccgg ttccgagcag tcccttcagc
     2221 agctgcagcg ctcgggtagc agcaccaact tcacggtcgc ggagaacggc aaaacgccga
     2281 tcatcgacat caccgcgacg tcgcgttcgc cgcaacaggc gctggcgacg acgaaccagc
     2341 tgatctcgat cattcaggcc gacctccaga cgcgtcagca cccctacgcg ccgaacaagg
     2401 caaaccagat cacggctccc gtcctgtcgc cgtcggttct cggcgcgctc gacaagagcg
     2461 cgaagaagaa ggctctgacg atcgcgatcg gtgtcggcat cgtcttcggc atcatcgtga
     2521 ctctgatcat cgacggtgtc ttgagagcac ggcagcgtgg tcgtgaagcg aagaccgatt
     2581 gggagggacc cgtgctcgac cgggagcct
//
LOCUS       DASWVF010000201         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_438625, whole genome shotgun sequence.
ACCESSION   DASWVF010000201 DASWVF010000000
VERSION     DASWVF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438625"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGD55_03585"
     CDS             <1..502
                     /locus_tag="VGD55_03585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0869458.1"
                     /translation="AAANAMFDIDVVDLAELALPFVDEPNHPTLRQYTKQHTLDWSAK
                     VATAEAFVFVMPEYNFAFNAPLKNAIDFLFHEWHYKPVGFVSYGAVSGGLRAVQMMKQ
                     VVTAVKMFPVYEAVSIPFVRKRINESGAFEADETLAASAATMLTELHRVAIGMNALRQ
                     PTAPGN"
     gene            complement(473..1087)
                     /locus_tag="VGD55_03590"
     CDS             complement(473..1087)
                     /locus_tag="VGD55_03590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869459.1"
                     /translation="MATFTGPVALRPMTRDDFPALAAWLRMPHVEAWFPWEQGPADPA
                     EAVEVEYGPCIDGLDPTELFVIEVDAGRVGFIQRYRISDNPAWAAALAPALDASAAVG
                     IDNAIGFDYAIGVVEATGRGIGSEAIRQLAVDTFDRYADVDGIVVAMQQANRASWRAL
                     ERVGFERRWAGLLDSPDPSDAGPSYVYALARDVAASVAGGGGLA"
     gene            1183..1917
                     /locus_tag="VGD55_03595"
     CDS             1183..1917
                     /locus_tag="VGD55_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869460.1"
                     /translation="MPSLAAVPPIAGAIGQARLLVVDDEPNILELLSATLRFSGFEVA
                     SAATGHEALETTQTFKPDLIVLDVMMPGIDGFDVARRLRAGGVRTPVLFLTARDSDED
                     KVTGLTIGGDDYVTKPFSLEEVIARIRSILRRTRGADGEAGTSVLRLGDISLDEDSYE
                     VLKASEPVELSATEFKLLRYFLRNPGRVLSKTQILEHVWSYDFGGDANIVETYVSHLR
                     RKIDTTQPRLLHTLRGMGYALRVPRG"
     gene            1943..3613
                     /locus_tag="VGD55_03600"
     CDS             1943..3613
                     /locus_tag="VGD55_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0869461.1"
                     /translation="MSTADPGVEQTADAAPRRPLPVRWGRAIAARSRQVPLRVRLVLL
                     TCALVTGALISTGFAATDLLRNSLMNRVDSQLISASHQLTDEVPRRLGGSRGPTAPGA
                     GATVPAIPAVGGQADGDAPGGSAPVSCAATNTTDSTTDTRPRLPSPYYFQTSDNAGNP
                     TGFSSIPLDQTDCGPAVPSLDIAAVKARLGKPFTVPSRNGGGRWRVVVSVTANGNGSV
                     AVATPLNGVDSTLNQLVEFDVIIGAAVLIVLAGLSYIAVRRSLRPLVDVEHTAAAIAA
                     GDLSRRVPQSDPRTEVGKLAGALNGMLSQIESAFRAREASEQEARTSEHRMRRFITDA
                     SHELRTPLTSIRGFAELYRMGAVPEESDVDRVMSRIENESTRMGLLVDDLLLLARLDQ
                     QRPLERELVDLETIAADAVHDAQAVSPGRDVQLQLSTDEPVLVVGDDARLRQVVGNLV
                     TNALTHTPQSASVTVGLSIDDTKPERFAVLEVADSGPGLSPKDAQRVFERFYRVEESR
                     TRANGGSGLGLSIVAALTAAHGGTADVETKLGIGSRFRIRLPLADTAV"
     gene            complement(3659..4939)
                     /locus_tag="VGD55_03605"
     CDS             complement(3659..4939)
                     /locus_tag="VGD55_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY0869462.1"
                     /translation="MSTVVSDYRHRAVEAVDAMNVALDSFAGASLWSMSTRDLAALVV
                     GVEKVARRVAAVQVQVLGQADGGLVRTLTAAKSTEVWLRGAADVATWVGRARLGLGQQ
                     LAQRPVVAAAFAAGQISQDAATAVCEAVAALPASVPAALTGEVEQLLVGVGRDEGTRA
                     VVRRAMAISQQFAPQILEDAERWAREHRFLSLTTRHDGTVAVRGLLDKETGALALAVL
                     SPLAAPAPAIDGIPDPREAGARYADAFAQLCQVATSTLPDVRGERPTALVTMDLETLQ
                     GAAASAGLGMLDTGVSLSVEATRKMLCDANIIPIVLGGRGEALDIGRATRSIPTGIRR
                     ALIARDRGCAFPGCDRPPSWCDAHHITHWSAGGATAICNLCLLCSHHHDTVHHDGWDI
                     QMITAMPWFLPPAWIDSTRTPRQHSRHKITRLRT"
BASE COUNT          914 a         1804 c         1587 g          829 t
ORIGIN      
        1 ggccgctgcc aatgcaatgt tcgacatcga cgtcgtcgat ctggccgagc ttgcgttgcc
       61 gttcgtcgat gagccgaatc atccgacgtt gcggcagtac acgaagcagc acacgctcga
      121 ctggagcgcg aaggtagcca ccgctgaagc attcgtcttc gtgatgcctg aatacaactt
      181 cgccttcaat gcaccgctga agaacgcgat cgacttccta tttcacgaat ggcactacaa
      241 gcccgttggg ttcgtgagct acggcgccgt ttcgggcggc ctgcgagcag tgcagatgat
      301 gaagcaggtt gtcacggcgg tgaagatgtt ccccgtgtac gaagcagtct cgattccctt
      361 cgtccgcaag cgaattaacg agtccggtgc attcgaggcg gacgagacac ttgcggcctc
      421 ggcggcaacg atgctgaccg aacttcatcg tgtcgcgatc ggcatgaacg cattacgcca
      481 gcccaccgcc cccggcaact gacgccgcca cgtcgcgcgc cagcgcgtac acgtagctcg
      541 ggccggcatc gctcggatcc ggtgagtcga gcaacccagc ccatcgtcgt tcgaagccga
      601 ctctctcaag tgcccgccac gacgcccggt tcgcctgctg catggccacc acgatcccgt
      661 cgacatcggc gtacctgtcg aacgtgtcaa cggcaagctg ccggatcgcc tcgctgccga
      721 tgccgcgccc ggtggcttcg accacgccga tcgcgtagtc gaagccgatg gcgttgtcga
      781 tcccgaccgc ggccgacgcg tcgagcgccg gtgcgagcgc ggccgcccat gccgggttgt
      841 cgcttatccg gtaccgctgg atgaagccga cacgacccgc gtcgacttcg atgacgaaca
      901 gctcggtcgg gtcgagtccg tcgatgcagg gcccgtactc gacctccacg gcttccgccg
      961 ggtccgcggg cccctgttcc cacggaaacc atgcctcgac gtgcggcatc cgcagccagg
     1021 cggcgagtgc cgggaagtcg tctcgggtca tcggccgcag agctactggc ccggtgaagg
     1081 tcgccataag agcagtatcg ggcgtgatac ggccgcgtga atcgcagcgc gcacttggcg
     1141 gacgttcagc aaactcccag gcttgagcgc gaagatgctc gtatgcccag tctggccgca
     1201 gttcccccga tcgcgggagc catcggtcaa gcacggctgc tcgtcgtcga cgatgagccg
     1261 aacattctgg agttattgtc ggccaccttg cgctttagcg ggttcgaagt cgccagcgca
     1321 gcaacgggcc acgaggccct cgagacgacc cagaccttca aacccgatct gatcgtgctc
     1381 gatgtcatga tgccgggcat agacggcttc gacgttgcgc gacgcctccg ggccggcggt
     1441 gtccgcaccc ccgtgctctt cctcactgca cgcgacagcg acgaggacaa ggtcaccggc
     1501 ctgaccatcg gcggcgacga ctacgtcacc aagccgttca gcctcgaaga agtcatcgcg
     1561 cgcatccgca gcatcttgcg acgcacaaga ggggcggacg gcgaggcggg cacgtccgtc
     1621 cttcggctcg gcgacatcag cctcgacgaa gacagctacg aggtgctgaa ggcgagcgag
     1681 ccggtcgagc tgtcggcgac cgagttcaag ctcctgcgct acttcttacg caaccccggc
     1741 cgggtgctgt cgaagacgca gatcctcgag cacgtctggt cgtacgactt cggcggcgac
     1801 gccaacatcg tcgagactta cgtctctcac ttgcgccgca agatcgacac cacgcagccg
     1861 cggctgctgc acacgttgcg tggcatgggc tacgccctac gggtcccacg cggatgacat
     1921 ccacatcgca ggtgcaactg aggtgagcac tgccgatccc ggcgtcgagc agacggcaga
     1981 tgccgctccc cgtcgaccac tcccggtgcg gtggggccgc gccatcgcgg cacgttcgcg
     2041 gcaggtcccg ctgcgggtgc ggctcgtcct tctcacctgc gcgctggtga ccggcgcgtt
     2101 gatctccacc ggattcgcag ctaccgacct gctgcgtaat tcattgatga accgggtcga
     2161 tagccaactc atcagcgcga gccaccagct gactgacgag gttccgcgtc gcctcggcgg
     2221 gtctcgcggt cccacagcgc cgggagcggg agcgaccgtc ccggccattc ctgcggttgg
     2281 tgggcaggca gacggcgacg cgcctggcgg tagtgcgccc gtctcatgcg cggcgaccaa
     2341 cacgacggat tcgacgaccg acacccggcc ccgcctgccg agcccttact acttccagac
     2401 gagcgataac gccggcaacc cgacgggatt cagcagcatc ccgctcgatc agactgactg
     2461 cggaccggcg gtgccgagcc tcgacatcgc ggcggtaaag gcccgcctcg ggaagccctt
     2521 cacagtgccg tcgcgcaacg gcggcggccg ttggcgagtg gtggtctcgg tcaccgcaaa
     2581 cgggaacgga agcgtcgcag tggcgacccc gctcaacggc gtcgatagca cgctgaatca
     2641 gctcgtggaa ttcgacgtca tcatcggagc cgcggtcctc atcgtgctcg cggggttgag
     2701 ctacatcgcg gttcggcgca gcttgcgccc actcgtcgat gtcgagcaca cggccgctgc
     2761 catcgctgca ggcgacttga gccgacgggt gccacagagc gatccacgca ccgaggtcgg
     2821 aaaacttgca ggcgcgttga acggcatgct gagccagatc gagtcggcgt tccgtgcacg
     2881 cgaggcatcg gagcaagaag cgcgtacgtc cgagcaccgc atgcggcgat tcatcaccga
     2941 cgccagccac gagttgcgga cgccgctcac ctccatccgc ggttttgcgg agctgtatcg
     3001 catgggcgca gtgcccgaag agtccgacgt cgaccgcgtg atgtcgcgga tcgagaacga
     3061 gtcgacccgc atgggattgc tcgtcgacga cctgctgctc ctcgcccgcc tcgatcagca
     3121 gcgtcccctc gaacgagaac tcgtcgacct tgaaaccatc gcggccgacg ctgtgcatga
     3181 cgcgcaagca gtctcgccgg gccgcgacgt gcaacttcag ctttcgaccg acgagccggt
     3241 ccttgtggtc ggcgacgatg cacggctgcg tcaagtcgtc ggcaacctcg tcaccaacgc
     3301 cctgacccac acgccacagt cggcgtcggt cacagtcggt ctcagcatcg acgacaccaa
     3361 gcccgagcga ttcgccgtgc tcgaagtcgc cgattcgggt cctggtctga gcccgaaaga
     3421 cgcgcagcgt gtgttcgaac gtttctatcg ggtcgaggag tcgcggacgc gcgccaacgg
     3481 tggcagcgga ctcggccttt cgatcgtggc cgcgctgact gccgcccacg gcggcacggc
     3541 agacgtggag acgaaactcg gcatcggctc gcgcttccgg atccgactac cgctcgcgga
     3601 caccgccgtc taaccgctcg cggacaccgc cgtccgccgc tccccgtctt tgcggccctc
     3661 acgtgcggag tcgggtgatc ttgtgtcggc tgtgttgtcg tggtgttctc gttgaatcga
     3721 tccatgccgg tggtaggaac cagggcatgg cggtgatcat ttggatatcc cagccgtcgt
     3781 ggtggacggt gtcgtggtga tgggagcaca acaggcacag gttgcagatc gcggttgccc
     3841 cgccggcgga ccaatgggtg atgtggtgcg cgtcgcacca tgacggtggc cggtcacacc
     3901 cggggaacgc gcatccgcgg tcgcgggcga tcaacgcgcg ccggatgccg gtggggatgg
     3961 agcgggtcgc ccgcccgatg tcgagggctt cgcctcgtcc gccgagcacg atcgggatga
     4021 tgttcgcatc gcacagcatt ttccgggtcg cctcgaccga gagcgacacc cccgtgtcga
     4081 gcatccccaa ccccgcgctc gccgcggcgc cttgcaaggt ctccaaatcc atcgtgacca
     4141 gcgccgtcgg ccgctcccca cgcacgtcgg gcaaagtcga ggtcgccacc tggcataact
     4201 gggcgaacgc gtcggcgtag cgggccccgg cctcccgcgg atcggggatg ccatcaatcg
     4261 cgggtgctgg tgcggccagc gggctgagca ccgctaacgc cagcgccccg gtctccttat
     4321 ccagcaaccc acgcaccgcc actgtgccgt cgtggcgggt cgtcaacgac aagaagcggt
     4381 gctcgcgtgc ccaccgctcg gcatcctcca agatctgcgg ggcgaactgc tgactgatcg
     4441 ccatcgcccg gcgcaccacc gcgcgggtgc cctcatcgcg gcccaccccg accagcaact
     4501 gctccacctc accagtcagc gccgccggca ccgacgccgg caacgccgcc accgcttcac
     4561 acacggcggt cgccgcgtct tggctgatct gccccgccgc gaacgccgcc gccaccaccg
     4621 gccgctgcgc caactgctga cccaacccca gccgggcccg ccccacccac gtcgccacat
     4681 cagcagcgcc acgcaaccac acctcagtcg acttcgccgc cgtcaaggtg cgcaccaacc
     4741 cgccatcggc ttgacccaac acctgcactt gtaccgcggc gacccgacgc gcgaccttct
     4801 ccaccccgac caccaacgcc gccagatcac gtgtcgacat cgaccacaac gacgcaccag
     4861 cgaagctgtc taacgcgaca ttcatcgcat caacagcctc caccgcgcga tggcggtaat
     4921 cgctgaccac tgtgctcata aagcaaaacc taccgaccac caccgacaga aagtgccagc
     4981 ccgcaattcc tgtggattaa ggaaaaaccg gcctgtgcac aacccaagaa gcgcgcgaca
     5041 tgttgaccga gcaaacgcgc agcgtcagcg cgctccgtca tgcaggatag aagttcgtca
     5101 gtcgttcgat gcgtccgtcg gagcggaact cgac
//
LOCUS       DASWVF010000202         6269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_132862, whole genome shotgun sequence.
ACCESSION   DASWVF010000202 DASWVF010000000
VERSION     DASWVF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132862"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGD55_03610"
     CDS             <1..402
                     /locus_tag="VGD55_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0869463.1"
                     /translation="ANLPGGAANWGGSWLGVPKAGKNQAAAEALAEWLTAPAQQITMW
                     TSQQHFPSSSTAAQDSSVAAATSDYFNKAPIGQIFGGSASKLKQQPIGPYDTQIQNDI
                     DNSLLNVANKGETPAKAFSEALANIAQVVGG"
     gene            584..1552
                     /locus_tag="VGD55_03615"
     CDS             584..1552
                     /locus_tag="VGD55_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0869464.1"
                     /translation="MTVLSGAGRRATPAAGRPGRLLKPRAGRVGSRAAYAYVAPFFIV
                     FGAFGLYPLLYTGWVSLHNVNLENLHHSSWVGLHYYVALWHNQFFWNALRNTITIGII
                     STVPQLCMALGLAQLLNYKMRARTFFRVAMLMPYATSVVASTLIFAELFGRDYGLINW
                     VLHFFGVHHINWTDGNRWQSQIAIAAIVTWRWTGYNALIYLAGMQSIPTDQYEAAAID
                     GANRWQQFWLVTIPSLRPTILFTIVISTIGAMQMFAEPYLFAGDGTTNGGNQHQYQTL
                     ALFMYQQGWTNFHLGQAAATAWTMLLLILVLVGVNAALARGRTGRI"
     gene            1705..2478
                     /locus_tag="VGD55_03620"
     CDS             1705..2478
                     /locus_tag="VGD55_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0869465.1"
                     /translation="MTIVAAFPLYYTLIIASHTNHEMAQTPPPFLPNASVFHNIRLAL
                     HEQPLFKAIVNTVIVAGSITIGTVAFCTLSGFAFAKLRFRGSGVLLALTVATIAIPPQ
                     LGVIPLFLLIAKLHWVNHLQSVVLPTLVSAFGVFFMRQYLANAVPTELLEAGWVDGAS
                     TFRIFRSIVLPIARPAMAVLGMLAFLAAWNDFFWPLIALTSRNPTVQIGLAGLGSGYI
                     PQQAVIMAGTLLCTFPVLIVFALLGRQIVGGIMQGAVKG"
     gene            2475..3920
                     /locus_tag="VGD55_03625"
     CDS             2475..3920
                     /locus_tag="VGD55_03625"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY0869466.1"
                     /translation="MTGIDTPDRMHLSLRDGSAFPSRFVWGTATAAYQIEGAASEGGR
                     TPSIWDTFSHTPGRVLGGETGDVAADHYHRFAADVALMAELGVSGYRFSTSWSRVIPT
                     GAGPVNGKGIDFYSRLVDELLAHGITPALTLYHWDLPQELQDFGGWTARDTAARLADY
                     AGAMATALGDRVKFWTTLNEPWCSAFLGYAAGVHAPGHTNGAEALSAVHHLLLGHGLA
                     ARALRAALPADAQIAITLNPAVARPATDSAEDQAAAFKVDGLQNRIWLEPLFHAQYPV
                     DVREFTSGVTDWSYVHDGDLAVIATPFDILGVNYYNPVVVGHYDGAGARANADGHGDG
                     AGDAWPGCGDVQFFDTPGRHTAMGWPVDASGLHDLLSRLWHDYHQPMAITENGAAYDD
                     VVDPDGAIHDEERVRYVHEHLDAVHRAIADGIDIRGYYLWSLLDNFEWAWGYSKRFGI
                     VRVDFETQERTIKDSGLFYGGIARTNTLPAL"
     gene            complement(3944..5044)
                     /locus_tag="VGD55_03630"
     CDS             complement(3944..5044)
                     /locus_tag="VGD55_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0869467.1"
                     /translation="MVAEGPPPRRLRAVATSGGETPGALGGDALSPLEHRPTVWAVAA
                     LAGVSKTTVSRVLTGSPRVSPHARIAVEKAIEKLNYVPNRAARALVTRRADTLALIVS
                     EPETRLFSEPFFAGTVRGINQELAQTDYAFVLLSAEGDSSRIERYIMNGHADGVILMS
                     LHRRGPLVRLLTQSRTPVVLTGRPFPGESVPYVDADNRAGATAAVHHLVERGRTRIAT
                     IAGPADMPVGIDRLDGYRDALPADSRKPWRLMVEHGDFSEDSGEIAMRALLDRVPDLD
                     AVFAASDLMAIGAMRALRAAGRTVPDDVAVVGFDDSHAARLANPQLTSVRQPMEEIGR
                     SLARLLLNEVRNPGHRSASLIVPTELIIRESS"
     gene            5184..>6269
                     /gene="xylA"
                     /locus_tag="VGD55_03635"
     CDS             5184..>6269
                     /gene="xylA"
                     /locus_tag="VGD55_03635"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY0869468.1"
                     /translation="MPTSLHPTPQDRFTFGLWTVGWPARDPFGDATRPALDPVETVHR
                     LAELGAYGVTFHDDDLIPFGSSESDRDKHIARFKDALAETGMKVPMATTNLFQHPVFK
                     DGGFTSNDRAIRRYALRKVMRNLDLAVELGAKTYVFWGGREGAETDSAKDVRAALARY
                     AEGIDMLAQYAIDNGYDIRFAIEPKPNEPRGDILLPTVGSALAFISTLEHADMVGVNP
                     EVGHEQMAGLNFVHSIAQALWMGKLFHIDLNGQRGIKYDQDLMFGHGDLLNAFFLVDL
                     LENGGVGGAPAYDGPRHFDYKPLRTEDIDGVWASAAANMRTYLLLKERAAAFRADPEV
                     QSALVAARVGELSEPTLGEGESYSHLLQ"
BASE COUNT         1085 a         2035 c         1989 g         1160 t
ORIGIN      
        1 gccaaccttc ctggtggcgc ggcgaactgg ggcggctcat ggctcggtgt gccgaaggcg
       61 ggaaagaacc aggcagccgc agaagcactg gccgaatggc tgaccgctcc ggcccagcag
      121 atcacgatgt ggacgtcgca gcagcacttc ccatcgtcat cgaccgctgc gcaggactcg
      181 tcggtcgcgg cagcgaccag cgactacttc aacaaggcac cgatcggcca gatcttcggc
      241 ggctcggcgt ccaagttgaa gcagcagccg atcggcccgt acgacactca gatccaaaac
      301 gacatcgaca actcgcttct caatgtcgcg aacaagggcg aaactccggc caaagcgttc
      361 agtgaggcac tggcgaacat cgctcaggtg gtcgggggtt agtcccaggt gctaggggtg
      421 ctcgcgccgg tcaccgcacg ctgggttgat cggcgcgagc actgccctta ggaagtccgg
      481 cgacgtcggt ggtccccctt ttctgccgat gtcgccggct tcatcatgac gggatcgcgc
      541 cgcacgcgtt cggttgtcga gtgctgctgc tgaaggggtt gcgatgactg tgctttccgg
      601 cgccggacgc cgcgccacgc cagcggcagg tcggccaggt cgcctgctga aaccgagggc
      661 aggacgcgtc ggctcgcgtg ccgcgtatgc ctacgttgca ccgttcttca tcgtgttcgg
      721 cgcgttcggc ctctacccac tgctctacac aggatgggta tcgcttcaca acgtcaactt
      781 ggagaacctg caccactcgt cgtgggtcgg cctccactac tacgtcgcac tgtggcacaa
      841 ccagttcttc tggaacgccc tcaggaatac gatcacgatc ggcatcatct cgacggtgcc
      901 gcagctgtgt atggcacttg ggctcgcgca gctcctgaac tacaagatgc gcgctcgcac
      961 gttctttcgc gttgcaatgc tgatgcccta cgcgacgtcg gtcgtcgcga gcacgctgat
     1021 cttcgccgag ctgttcggtc gcgactacgg tctgatcaac tgggtcctgc acttcttcgg
     1081 cgttcatcac atcaactgga cggacggcaa caggtggcag tcgcagatcg ccatcgctgc
     1141 catcgtgacc tggcgttgga ccgggtacaa cgcattgatc tacctcgcgg gtatgcagtc
     1201 gataccgacg gatcagtacg aagcagcggc gatcgacggc gcgaatcgct ggcagcagtt
     1261 ctggctcgtc acgattccat cgcttcgccc gacgattctc ttcaccattg ttatttccac
     1321 gatcggagcg atgcagatgt ttgccgagcc ttacctgttc gccggcgacg gtacaacgaa
     1381 cggaggcaac cagcatcagt accagacatt ggcattgttc atgtaccagc aggggtggac
     1441 gaactttcac cttgggcagg ccgccgccac tgcctggacg atgcttctgc tcattctcgt
     1501 gctggtcggc gtgaacgcgg cgctcgcccg cggccgcact ggccggatct agccggacta
     1561 ggggcgacga ccatgacctc ggatgcactc gagtcgacgg cgactgaaca gccggcggcg
     1621 ctgacggcca gaccccgccg cacacacggt cgtgtcggca ggcaacttac cgcaggtaaa
     1681 ggtagctata tcattctctc gcttgtgacg atcgttgccg cgtttccgct gtattacacc
     1741 ttgatcattg cttctcatac gaaccacgag atggcgcaaa cgccaccgcc gttcctgccg
     1801 aacgcatcgg tgttccacaa catcagactc gccctgcacg agcagccgtt gttcaaggcg
     1861 atcgtcaaca ccgtcattgt ggccgggtcg atcacgatcg gtacggtggc cttctgcacg
     1921 ctgagtgggt tcgcgttcgc caagctgcgg tttcgcggca gtggtgtatt gctggccctc
     1981 accgtcgcga cgatcgcgat tccaccgcag ctcggcgtga ttccactgtt cttgttgatc
     2041 gcgaaactgc actgggtcaa ccaccttcaa tcagtggtct tgccaaccct ggtgagtgcg
     2101 ttcggcgttt tcttcatgcg acagtacctc gccaacgcag tgcccacgga gctactcgag
     2161 gccggctggg tcgacggtgc gtcgacgttc cgcatctttc gttcgatcgt gttgcccatc
     2221 gcgcggcctg cgatggcagt gctcggcatg ctcgcgtttc ttgccgcgtg gaatgacttc
     2281 ttctggccgc tgatcgcgct caccagccgg aatccgaccg tgcagatcgg tcttgcgggt
     2341 ttgggctctg ggtacattcc ccagcaggca gtcatcatgg ccggcacgct cctgtgtacg
     2401 tttcctgtgt tgattgtttt cgccctgctc gggcgacaga tcgtcggagg cattatgcaa
     2461 ggcgctgtca aaggatgacg gggatcgaca caccagaccg gatgcatctg tcattgcgcg
     2521 acggctcggc tttccccagc cgcttcgtct ggggcaccgc gactgccgcg tatcagatcg
     2581 agggtgcggc aagtgaaggc ggccgcacgc cgtcgatctg ggacacgttc agccatacac
     2641 ctggcagggt tctcggtggt gaaaccggcg acgtagcggc cgaccactac caccggtttg
     2701 ccgccgacgt ggccctcatg gccgagctgg gggtgtcggg gtaccggttc tcgacgtcgt
     2761 ggtcgcgcgt cattccgaca ggcgctggcc cggtcaacgg caagggcatc gacttctact
     2821 cgcgactggt cgacgaacta ctcgctcacg ggatcacgcc tgcgctcacc ctttaccact
     2881 gggatttgcc gcaggagttg caagacttcg gcggttggac cgcgcgcgac accgccgcgc
     2941 gactggccga ctacgcgggt gcgatggcga cagcgctcgg tgaccgggtc aagttctgga
     3001 ccacgctgaa cgaaccctgg tgctccgcgt ttctcggcta cgcggccgga gtgcacgcac
     3061 ccggccacac aaatggcgcc gaggctctaa gtgcggtgca ccacttgctc ttgggccacg
     3121 gtctagccgc ccgggcgttg cgggcggcgc tgccggccga tgcgcagatt gcgatcacac
     3181 tcaatccagc cgtcgcccgc ccggcgaccg actcggccga agaccaggct gctgccttca
     3241 aggtcgacgg cctgcagaat cgcatctggc tcgaaccgct cttccacgcc cagtacccag
     3301 tcgacgtgcg cgagttcacg tcgggtgtta ccgactggag ctacgtgcat gacggcgacc
     3361 tggccgtcat cgcgacgccg ttcgacatcc tcggggtcaa ctactacaac ccggtcgtgg
     3421 tcgggcatta cgacggcgcg ggcgcccgcg ccaatgccga tggtcatggt gacggggctg
     3481 gcgacgcgtg gcccggctgt ggcgacgtgc agttcttcga cacccctggc cggcacacgg
     3541 cgatggggtg gccggtcgac gcgagcggcc tgcacgatct gctctcacgg ctgtggcatg
     3601 actatcacca gccgatggct atcacggaga acggtgcggc ctacgacgac gtcgtcgacc
     3661 ccgatggcgc gatccacgac gaggagcggg tgcgctacgt ccacgagcat ctcgacgccg
     3721 tgcatcgtgc gatcgccgat ggcatcgaca ttcgcgggta ctacctgtgg tcgctcctcg
     3781 acaacttcga gtgggcctgg gggtactcca agcgcttcgg catcgtgcgc gtcgatttcg
     3841 aaacacagga gcggaccatc aaggacagcg gactcttcta tggcggcata gcgcggacga
     3901 acacgctacc tgcgttgtaa gtgcgcctag ctcgactctc tgcctagctc gactctctga
     3961 tgatgagctc ggtcggaacg atcagggacg ccgagcggtg gcccggattg cggacctcgt
     4021 tcaagagcag tcgagcgagc gaccgcccga tctcctccat cggctgacgc acgctggtca
     4081 actgggggtt cgcgagccgg gcagcgtggc tatcgtcgaa gccgacgacg gccacgtcgt
     4141 ctgggaccgt gcggccggcg gcccgcaatg cacgcatcgc gccgatcgcc atgaggtcgc
     4201 tcgccgcaaa caccgcgtcg aggtcgggca cccggtcgag caaggcacgc atcgcgatct
     4261 caccgctgtc ttcggagaag tcgccatgct cgaccatgag ccgccacggc ttgcgactgt
     4321 ctgctgggag ggcgtcgcgg tagccgtcga gccggtcgat gccgacgggc atgtcggccg
     4381 gcccagcaat ggtcgcgatg cgcgtacgac cgcgctcgac aaggtgatgc acggctgcgg
     4441 ttgccccggc tcggttgtcg gcatcgacgt acgggacgct ctcgccgggg aacgggcggc
     4501 cggtcagcac aaccggcgtc cggctctggg tgagtaatcg caccagcggc ccgcggcggt
     4561 gcagtgacat caagatcacg ccgtcggcgt gcccgttcat gatgtaacgc tcgatcctgg
     4621 agctgtcacc ctctgcgctg agcagcacga acgcgtagtc ggtctgcgcg agttcctggt
     4681 tgatgccgcg gaccgttccg gcgaagaagg gctcggaaaa cagtcgagtc tcgggctcgg
     4741 agacaatcag cgccagcgtg tcggctcggc gggtgactag cgcgcgggcg gcgcgattcg
     4801 ggacgtagtt gagcttctcg atcgccttct cgaccgcgat ccgcgcatgc ggagacactc
     4861 gcggcgagcc ggtgagcacc cgcgacaccg tcgtctttga caccccggcc agggcagcga
     4921 ccgcccagac cgtcgggcga tgctccaggg gtgacagtgc gtcaccgccc agcgcccctg
     4981 gggtttcgcc tccggaggtc gcaactgcgc ggaggcgtcg cggcggggga ccttcggcaa
     5041 ccatgcagtg atgatttcac aggagtgagc aagcgaacga cgtacgctaa ggcccgcgaa
     5101 cgttcccggt ttccgttgac cgcgtgcggc gcgacaaata tgttgagctg gccaacaaat
     5161 cacccgcgtc tgaggagatc cccgtgccga cgtcgttgca tccgacgccc caagaccgct
     5221 tcaccttcgg gctttggacc gtcggttggc cggcgcgcga cccgttcggc gacgcaaccc
     5281 gccctgcact cgacccagtc gagaccgtgc accgacttgc cgagctaggg gcctacggcg
     5341 tcactttcca tgacgacgac ctgatcccgt tcggcagcag cgagtcggac cgcgacaagc
     5401 acatcgcccg gttcaaggac gcactcgccg agaccggcat gaaggtgccg atggcgacga
     5461 cgaacctttt tcagcaccct gttttcaagg acggcggctt cacaagcaac gaccgggcga
     5521 tccgccggta tgcgctgcgc aaggtcatga gaaacctcga tctcgcggtc gaactcggcg
     5581 ccaagaccta tgtgttctgg ggtggccgtg agggtgccga gaccgactca gccaaggacg
     5641 tcagagctgc gctcgcccgc tatgccgagg gcatcgacat gctcgcgcaa tacgcgatcg
     5701 acaacggata cgacatcagg ttcgccatag agccaaagcc gaacgagcct cgtggcgaca
     5761 tcttgctccc gacggtcggt tccgcgctgg ccttcatctc caccctcgag cacgccgaca
     5821 tggtcggcgt gaaccccgag gtcggccacg agcagatggc cggcttgaac ttcgtgcatt
     5881 cgatcgcgca ggccttgtgg atgggcaagc tcttccacat cgacctcaac ggccagcgcg
     5941 ggatcaagta cgaccaggac ctcatgttcg gccacggcga cctgctgaac gcgttcttcc
     6001 tcgtcgacct gctcgaaaac ggcggcgtgg gtggggcgcc cgcatacgac ggtccgcgcc
     6061 atttcgacta caagccgttg cgcacggaag acatcgacgg cgtatgggca tcagctgcgg
     6121 cgaacatgcg tacctacctg ttgttgaagg agcgcgccgc ggcgtttcgg gccgaccccg
     6181 aggtgcaatc cgcgctcgtg gccgcgcgcg tcggtgagtt gtccgaaccg acgttgggcg
     6241 aaggcgagtc gtacagccac ctgctgcaa
//
LOCUS       DASWVF010000203         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_260394, whole genome shotgun sequence.
ACCESSION   DASWVF010000203 DASWVF010000000
VERSION     DASWVF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260394"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGD55_03640"
     CDS             <1..527
                     /locus_tag="VGD55_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0869469.1"
                     /translation="VVIQRLARHNGVVTELLDFCAGLPRQSIAAGEALIEEGVRHDGM
                     LVLVSGSVSVERDGVPFARVDTPGAVFGEMSVVLDKVATATVRARSDVEVYAIAEPLD
                     FLRDKPGAALAVLRMTAARLDGLTQYLVDVKRQYADRDDHLGMVDGILDSLVHHQPRQ
                     ARTGSVRDPEVSSD"
     gene            complement(514..1233)
                     /locus_tag="VGD55_03645"
     CDS             complement(514..1233)
                     /locus_tag="VGD55_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0869470.1"
                     /translation="MRILVASDLHYRLPHYDWLVKSSGDVDVVALAGDLADVVNPVPF
                     EVQTVVLERYLMTLASRATVLAASGNHDLDGPGDLGEQVAGWLRGLRTETLRCDGASI
                     DVGGVRFTVCPWWDGPATRDALAAQLAAAAIDRPPIWVWIYHAPPAGTVLCNDGRRTF
                     PDHELAAWIEQYQPAMVLCGHIHQAPWIDGGSWHARLGTTWVFNAGKQVGPIPPHITI
                     DTELGTAQWYGVFSSETISLS"
     gene            1412..>2288
                     /locus_tag="VGD55_03650"
     CDS             1412..>2288
                     /locus_tag="VGD55_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY0869471.1"
                     /translation="MGTRWHRGATLLGTGIVSTGLIFGSSTMAAAAPHDSADDVPIAQ
                     TNLVSDQVGHAPLVDANLVNPWGMSYGAATPVWVSDNGKDVSTLYSGNTFPPAFTKAN
                     LTVSIPGGEPTGQVFNSSTTDFVVTNGTSGPAKFIFASEAGWITGWNPNVPTTGSTQA
                     TPAKQIPNAVFKGLALANFDGANYLYATDFNHAKVDVFDSTFGLQHWNGAFQDSAIPS
                     GYAPFNVQLLNGSLYVTYAKQDAAKHDDFAGKGHGFVDVFTTGGQLVKRLVRNGDLNS
                     PWGLAIAPSSWGELAG"
BASE COUNT          409 a          749 c          741 g          389 t
ORIGIN      
        1 gcgtagtcat ccaaaggctg gctcgacaca atggggtcgt gacggaactg ctcgacttct
       61 gcgccggcct gcctcggcag tcgatcgccg ctggtgaggc gctcatcgaa gagggcgtgc
      121 gtcacgacgg catgttggtg ctcgtctccg gatccgtcag cgtcgagcgc gacggcgtgc
      181 cgttcgctcg ggtcgacact ccaggggccg tgttcgggga gatgtcggtc gtgctcgaca
      241 aggtcgcaac cgcgacagta cgagcgaggt cggacgtcga ggtgtacgca atagcggagc
      301 cgctcgactt ccttcgcgac aagccgggtg ccgcgctggc cgtgttgcgc atgacggccg
      361 cgcgccttga cggcctgacg cagtacctcg tcgacgtcaa gcggcagtac gcagatcgcg
      421 acgaccacct cggcatggtc gacggcattc tcgactcgct cgtgcatcac cagccgcggc
      481 aggcccgcac ggggtcggtg cgcgaccccg aggtcagctc agactgatcg tttctgagct
      541 gaagactccg taccactgcg cggtgccgag ttcggtgtcg atggtgatgt ggggaggtat
      601 cggccctacc tgcttgcctg cgttgaaaac ccacgtggtt cccaggcggg cgtgccacga
      661 gccgccatcg atccacggtg cttgatggat gtggccgcac agcaccatcg cgggctggta
      721 ctgctcgatc cacgccgcaa gttcgtgatc ggggaacgtc cggcgtccgt cgttgcatag
      781 caccgtgcct gccgggggtg cgtgatagat ccacacccag atcggtggtc ggtcgatcgc
      841 ggcggccgct agctgcgcgg cgagcgcgtc gcgggtcgcc ggaccgtccc accacggaca
      901 cacggtgaag cgcacgccgc ccacgtcgat cgacgcgcca tcgcagcgca gtgtctcggt
      961 gcgcagcccg cgcaaccagc ccgctacctg ctcgcccaga tcgccgggac cgtcgagatc
     1021 gtggttgccg gaggctgcaa gaacggttgc ccgtgacgcc agcgtcatga ggtagcgctc
     1081 gagaacgacg gtctgcactt cgaacggaac cgggttcacc acatcggcca aatcgccggc
     1141 aagcgccacg acatcgacat ccccggaaga cttcaccagc cagtcgtagt gcggcagccg
     1201 gtagtgcagg tcagaggcca ccaggatgcg cacgggatga tcttgcctta cttccgcggg
     1261 aagcttgcat ggcctgtgct tgaacgattc ggggcgatcc gcgcgacacg catgacgact
     1321 gaaccgttcc catcggggat ccgtgtgccg ccattgagcc ggtggacgga gttgaacagg
     1381 cgactccgcc cggccgatca gaggaggcca catgggcacc agatggcata ggggagcgac
     1441 actgctcggc accggcattg tttcgacagg cctcatcttc gggtcgtcga ctatggccgc
     1501 cgccgcgccc cacgacagcg cagacgatgt accgatcgcc cagaccaatc ttgtctccga
     1561 ccaggtgggc cacgccccac tcgtcgatgc gaacttggtc aacccgtggg gaatgtccta
     1621 cggggcagcg accccggtgt gggtctcgga caacggcaag gacgtttcga cgctctacag
     1681 cgggaacact ttcccgccgg cattcaccaa ggccaacctc accgtgtcga tcccgggtgg
     1741 tgaacccacc ggacaggtct ttaactcatc gaccaccgac tttgtggtca ccaacggtac
     1801 gagtgggccg gcgaagttca tcttcgcctc agaagctggg tggatcaccg gctggaaccc
     1861 gaacgtcccg acgacgggtt cgacgcaggc gacgcctgcg aagcagatcc cgaatgcagt
     1921 gttcaagggt ctggcgctcg caaacttcga cggcgcgaac tacctctacg caaccgactt
     1981 caaccacgcg aaggtcgacg tgttcgacag caccttcggc ctccagcact ggaatggcgc
     2041 cttccaggac agcgcgatcc ccagtgggta cgcgcccttc aacgtccagc tcctgaacgg
     2101 cagcctctac gtgacgtacg ccaagcagga tgcggccaag cacgacgact ttgctggcaa
     2161 gggtcacggg ttcgtcgacg tgttcacgac cggtggtcag ctcgtgaagc ggctcgtccg
     2221 caacggcgac ctcaactcgc cgtgggggtt ggccatcgca ccgtcgtcat ggggtgagct
     2281 cgccggct
//
LOCUS       DASWVF010000204         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_209372, whole genome shotgun sequence.
ACCESSION   DASWVF010000204 DASWVF010000000
VERSION     DASWVF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209372"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGD55_03655"
     CDS             <1..622
                     /locus_tag="VGD55_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0869472.1"
                     /translation="GEAVVVQKEVLRVSTEETSQVNVGDGVLRDETVRTGLDSATRLV
                     MADSTNLSLGANASITLDRTVFDDEHKYRDIAIMMTSGAFRFVTGHSEKAAYKISTPL
                     ATIGVRGTILDILSERGKTTVVLQEGGSSVCTLTSQCIQLEQPGDTAVITASANGRRS
                     TIKKTNMPPWTFAAACAKSAGLCSVTDLAQASVPPPADDPGMLCGR"
     gene            610..1905
                     /locus_tag="VGD55_03660"
     CDS             610..1905
                     /locus_tag="VGD55_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869473.1"
                     /translation="MRALIVIKIVRSLVIAAALVSAYSLAMMEQAHANPECFVNCVDD
                     PPTHSTGSPRLETQFPPTGNGSEGTTGGGITGNGSAGTTGSGSTGATGSGSNCGPDEG
                     ACPTTTPTTSSDTSCDGDPCPPTTAAAPPPITGAASSGASIGGLVSQRTNQMITNRVL
                     GTVLLGVNEQVNCSDCVSAFGSAGSFSAGLHGRKELTNNLSLLAGVAYTQYSEGGYHV
                     TSAPIGAFALRYDFTDWGSSRPFFDIGTILTPYEKVRYSRSYTTSLGPVSLDSSTNSS
                     NYSVYGRAGWISRLSQRDEVAASVEIWQLWQRVAGYSDPATAFNPFDATIATGTDRTD
                     LVKIGGQWTHLFGSSVEGNINGGHVHSFASHSGIVATVTGDGVVTPTMGDQGWFEYGG
                     RLGFRIIQGWVADLFVNGTLGPQPVGNTIHGGVGLRINY"
BASE COUNT          373 a          710 c          625 g          326 t
ORIGIN      
        1 cggcgaagcg gtcgtggtgc aaaaggaagt cctccgcgtc tcgacggagg agaccagcca
       61 ggtcaacgtc ggcgacggcg tgctgcgcga cgaaaccgtc cgcaccggcc tcgacagcgc
      121 cacccggctg gtgatggcgg acagcaccaa tctctcgctc ggcgccaacg cctcgatcac
      181 gctcgatcgc accgtgttcg acgacgagca caaataccgt gacatcgcca tcatgatgac
      241 gtcgggcgca tttcgcttcg tcaccgggca ttcggaaaaa gccgcctaca agatctcgac
      301 gccgctggcg acgatcggcg tgcgcggcac catcctcgat atcctttcgg agcgcggcaa
      361 gaccacggtg gtgctgcagg aaggcggctc ctcggtctgc acgctcacgt cgcagtgcat
      421 ccagctcgag cagccgggcg acaccgcggt catcaccgcg tccgccaacg gccggcgcag
      481 caccatcaag aaaaccaaca tgccgccctg gacgttcgcc gccgcctgcg cgaaatcggc
      541 cgggctttgc agcgtcaccg atcttgccca agccagcgtc ccaccaccgg ccgatgatcc
      601 cggcatgctg tgcgggcgct gatcgtgatc aagatcgtac gcagccttgt gatcgccgcc
      661 gcgttggtct cggcatattc gctagccatg atggagcagg cccacgcgaa tcccgagtgc
      721 ttcgtgaatt gcgtggacga tccgccgacg cactcgacag ggtccccgcg gctggagacg
      781 cagttcccgc caacaggaaa cggtagcgaa gggaccaccg gcggtgggat caccggcaac
      841 ggcagcgctg gaacaaccgg cagcggcagc accggcgcta ctggtagtgg aagcaattgc
      901 gggccggacg agggggcttg cccgacgaca acgccgacga cgtcatctga tacgtcctgc
      961 gacggcgacc cctgcccgcc aacaacggca gcagccccgc caccgatcac cggcgcggcc
     1021 tccagcggcg cctcgatcgg cggtctcgtc agtcagcgca ccaaccagat gatcaccaac
     1081 cgtgtgctcg ggaccgtgct gctcggcgtc aacgagcagg tcaattgcag cgattgcgtc
     1141 agcgccttcg gctcggcggg atcgttttcg gccggccttc acggccgcaa ggaactgacc
     1201 aacaacctgt cgctgctcgc cggcgttgcc tacacgcaat acagcgaagg cggctatcac
     1261 gtcaccagcg cgccgatcgg cgccttcgcg ctgcgctacg acttcaccga ctggggatcg
     1321 tcgcggccgt tcttcgacat cggcaccatc ctgacgccgt atgagaaggt gcgctacagc
     1381 cgcagctata cgacgagcct cggtccggtg tcgctcgaca gttcgaccaa cagctcgaac
     1441 tattcggtct atggccgggc cggctggatc agccgcctct cacagcgcga tgaagtcgcc
     1501 gcctccgtcg aaatctggca gttgtggcaa cgcgtcgccg gctattcgga tccggccacg
     1561 gcgttcaatc cgttcgacgc cacgatcgcc accggcaccg accgcaccga tctggtcaag
     1621 atcggcggcc agtggaccca tctgttcggc tccagcgtcg agggcaacat caacggcggc
     1681 catgtgcact cctttgcctc gcattccggc atcgtcgcca ccgtcaccgg cgatggcgtc
     1741 gtgacgccga ccatgggcga ccagggctgg ttcgagtatg gtggccggct cggcttccga
     1801 atcatccagg gctgggtcgc cgacctcttc gtcaacggca cgctgggccc gcagccggtc
     1861 ggcaacacca tccatggcgg cgtcggcttg cggatcaact actagcgcgc ggccgcggac
     1921 ccgtccgtgg cagaatggcg ggtcgacacc gctttcgtgt gcgacaattc tccgtttgac
     1981 cacctcaacc cggcaggcaa gactacttgc catacgtctg ggagagccgc caag
//
LOCUS       DASWVF010000205         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_336574, whole genome shotgun sequence.
ACCESSION   DASWVF010000205 DASWVF010000000
VERSION     DASWVF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336574"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGD55_03665"
     CDS             complement(<1..572)
                     /locus_tag="VGD55_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0869474.1"
                     /translation="MTGKAAAAAVQEDRASSEVSAGSIPAGTPTTETDVIVVGAGPSG
                     SATAAHLAQAGLNVALLEKSAFPREKVCGDGLTPRAVTQLVKLGIDTSAEAGWLQNKG
                     LRILAGGMRVELPWPDLSAFPSYGLVRTRLDFDQLLARRAQKLGVALHEQTPATQPVV
                     DDRSGRVVGVMARPAGAEREVYFKAPVIVAA"
     gene            complement(732..1445)
                     /locus_tag="VGD55_03670"
     CDS             complement(732..1445)
                     /locus_tag="VGD55_03670"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HEY0869475.1"
                     /translation="MTPTRADLAKQPAEVAAMFDDVAARYDLVNDVLSLGRDRGWRKV
                     VAKAVGASKGERVLDVAAGTGTSSASFTTAGADCVACDFSLGMLRQGARQRGGAAAGG
                     VRFVAGDALALPFADESFDAVTISFGLRNVHDVDAGLRELLRVTKPGGRLVICEFSSP
                     VWTPFRRVYDGYLGVMPAIVKKVSSNPDSYVYLAESIRAWPAQEELASRIAGAGWERV
                     AWRNPTGGIVAVHRASRSA"
     gene            complement(1442..2455)
                     /gene="add"
                     /locus_tag="VGD55_03675"
     CDS             complement(1442..2455)
                     /gene="add"
                     /locus_tag="VGD55_03675"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0869476.1"
                     /translation="MSYPKIELHVHLEGTVRPRTLLDMAARNELPLPVDTIEGVENLY
                     EFTDFAHFIEVWILTTNVMRTAHDFRQIVVDYAAEAASHGAIYLEAIFSPIERVMRGV
                     SWDDLFEGYCDGAQQAEEDHGVVVRLTPDSYRGADVGLTEELAARAIKYKDRGVVGLG
                     LGGPEANFPPEPYAKAFEIAKAGGLASLPHAGESAGADSIRATMAALQADRIRHGIRA
                     IDDPDLMAELADKQIVLDVCPTSNLRTRSVRDLASHPLPRLIEAGISCSLNTDDPAMF
                     GTDLGHEYEIAQHLGVRPQELFEAGLHGAMCDEKTKARLRDVADNCDWTALAAPASDG
                     QRA"
     gene            2679..3527
                     /locus_tag="VGD55_03680"
     CDS             2679..3527
                     /locus_tag="VGD55_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869477.1"
                     /translation="MSRAQLGDAGYDSDAVRRRVATGEWQQVGNAFVLHSGVPTSDQL
                     QWAAVLTAPGLVAITARTAAKQYGLRGFELTAVDVLVTHSDGMPPRIDGVHWHVSRRF
                     SERDIALGFGLPMVSRGRAVVDAAAWTPRPRIACALLAAAVQQGVVPTAALRRELAAA
                     GAVRHSRILGSIMADIEGGADSLSEIDFVKLARKAGLPPPIRQSIRVDSAGRRRYLDA
                     DFGTFAVEVDGGLHLLAQNYWYDARRQNDLVLGGDHILRFPSIAIRLEEDVVLAQLRR
                     AHQVFG"
     gene            complement(3602..>4267)
                     /locus_tag="VGD55_03685"
     CDS             complement(3602..>4267)
                     /locus_tag="VGD55_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869478.1"
                     /translation="IAPLKETGAVWLEVMETAHGLGVQSTATFMMGTGETNAERIEHL
                     RVIRDVQDRTGGFRSFIPWTYQPQNNHLKGRTLATPLEYLRLLAVARLFFDNIAHLQV
                     SQLTLGKEVGQLGLHYGADDLGSVMLEENVVSSAGARHRWNLTELIHLIRSADRVPAQ
                     RDTLYNHLRVHWTPADDPEPDERVASHFASTAPGAQRAQAEGAQRAQAEGAQRVSLPL
                     VAS"
BASE COUNT          687 a         1417 c         1459 g          704 t
ORIGIN      
        1 gctgcgacga tgaccggggc cttgaaatag acctcgcgtt cggcaccggc agggcgtgcc
       61 atcacgccga cgacccgacc cgaacgatca tcgacgaccg gctgcgtcgc gggcgtctgc
      121 tcgtgaaggg cgacgccgag cttttgggcc cgccttgcca gcagctggtc gaagtcgagc
      181 cgggtgcgga cgagaccata gctagggaac gcagaaagat cgggccacgg cagttcgacc
      241 cgcatgccac cggccaggat ccgcaggccc ttgttctgaa gccacccggc ctcggcgctg
      301 gtgtcaatgc cgagcttgac gagctgcgta acggctcgtg gtgtcagccc gtcgccacaa
      361 accttctcgc gcgggaaggc ggacttctcg aggagggcga cgttcaggcc ggcctgggcg
      421 aggtgggccg cggtcgctga ccctgatggg ccagcaccga ccacgatcac gtcggtctcg
      481 gtggtcggcg tcccggctgg aatcgaacca gcggagacct cagacgacgc gcgatcttct
      541 tgaactgcag cggcggctgc cttgcctgtc acggattcac cacctaagcc gaagcgggta
      601 cgccgcgcgc ctaacgcgtg gttgcctgtt caacgcgagc gtttgtgaac gggttcacga
      661 gagcggcggc agaagtctat gccgtcggcg ggcaggctag cgcgccggtc gcgaggcccg
      721 ctgcagcggc gtcacgcgga gcgggatgcg cggtgaactg ccacgatccc gcccgtcggg
      781 ttgcgccagg caacccgctc ccaccccgcc ccggcgatgc ggctggcgag ttcctcctgc
      841 gcgggccaag ccctgatcga ctcggccagg tagacgtagg agtcggggtt cgaactgacc
      901 ttcttcacga tcgcgggcat gaccccgagg tagccgtcgt agacccggcg aaacggggtc
      961 cagaccggcg agctgaactc gcagatcacc aggcgccccc ccggtttggt cacgcgcagc
     1021 aactcgcgca ggccagcatc gacgtcgtgc acgttgcgga ggccgaacga gatcgtgacg
     1081 gcgtcgaacg actcgtcggc gaagggcagc gcgagcgcat cgccggccac gaaccggact
     1141 cccccggcgg ctgcgccacc tcgctggcgg gcgccctggc ggagcatccc gagcgagaaa
     1201 tcgcaggcca cgcagtcggc gccggccgtc gtgaaagacg ccgacgaggt gcccgtgccg
     1261 gcggcgacgt cgagcactcg ctcacccttg ctggcgccca cggccttggc gaccaccttg
     1321 cgccaaccgc ggtcgcgccc caaggacagg acgtcgttca ccaggtcgta gcgcgcggcc
     1381 acgtcgtcga acatggccgc gacctcggcg ggctgcttgg ccaggtcggc gcgagtcggt
     1441 gtcacgcgcg ttgcccatcg cttgcgggtg cggcgagcgc ggtccagtca cagttgtccg
     1501 cgacgtcccg caatcgggct ttggtcttct cgtcgcacat cgcaccatgg agtcccgctt
     1561 caaaaagctc ctgcgggcgg acgccgaggt gctgggcaat ctcgtattcg tggccgagat
     1621 cggtgccgaa catcgcaggg tcgtcggtgt tgagcgagca ggaaatgccg gcctcgatga
     1681 gccttggcaa cggatggctg gccaggtcgc gcacgctgcg ggtgcgcagg ttggaggtcg
     1741 gacatacgtc gagcacgatc tgtttgtccg ctagctcggc catcaggtcg gggtcgtcga
     1801 tcgcgcggat cccgtgcctg atgcggtcgg cttggagcgc ggccatcgtg gctctgatcg
     1861 agtcggcgcc ggccgattca cccgcgtgcg gaagcgacgc gagcccacct gcctttgcga
     1921 tctcgaacgc tttcgcgtaa ggctccggcg ggaagttcgc ttcagggccg ccaaggccga
     1981 gacccacgac gccgcggtct ttgtacttga ttgctctcgc ggccagctcc tcggttagtc
     2041 cgacgtccgc cccgcgatag gaatcgggcg tgaggcggac gacgacgccg tggtcctcct
     2101 cggcttgctg ggcgccgtcg cagtagccct cgaagaggtc gtcccagctc acgccgcgca
     2161 tgacgcgctc gatcggggag aaaatcgcct cgaggtagat cgcgccgtgt gaagccgctt
     2221 ccgccgcgta gtcgaccacg atctggcgga agtcgtgggc ggtgcgcatg acgttggtcg
     2281 tgaggatcca gacctcgatg aaatgcgcga agtcggtgaa ctcgtagagg ttttcgacgc
     2341 cctcgatcgt gtcgaccggc agcgggagct cgttgcgggc ggccatgtcg agcagggtgc
     2401 gtggtcggac cgtcccctcg agatggacgt gcagctcgat cttcggatag ctcaccacgg
     2461 cgcccatctt ggcagggccg gattctggcc aaccagcgcc cgcccgccgc cgtcccgtat
     2521 ggacttggaa gcctgtggac gcccaagatc gcgtccacag cttgccaact ttcgcggctc
     2581 cgctggctgg gctgcccaaa cctgagtaaa tgccaaagcg ttcagcgcac cagaaccctc
     2641 tccgcctcgc cgacatcgct gccacacaag ccacggtcgt gagccgagca cagctcgggg
     2701 atgcggggta cgacagcgac gccgttcgac gacgtgtcgc aacaggtgag tggcagcagg
     2761 tgggcaacgc attcgtcctg cacagcgggg tcccgacgag cgaccagctg caatgggcgg
     2821 cggtcttaac ggcgcctggt ctggtcgcga tcacggcgcg caccgcagcg aagcagtacg
     2881 gcctgcgtgg gtttgagctg acagccgttg acgtattggt cacgcatagc gacggcatgc
     2941 cgccgcggat cgacggcgtc cattggcacg tgtcgagacg attcagcgag cgggacattg
     3001 cgctcggatt cggactacca atggtgtccc ggggtcgcgc ggttgtcgac gccgcggcgt
     3061 ggacgcctcg gccgcggatc gcttgtgcgc tcctagctgc cgcggtccag caaggcgtcg
     3121 tgccgacggc ggcgctacgg cgggaactcg cggcggcggg cgccgtacgt cacagtcgaa
     3181 tccttggttc gatcatggcc gatatcgaag gcggcgccga ctctctctcg gagatcgact
     3241 tcgtcaaact cgcgcgcaag gcgggcctgc cgccaccgat cagacagtcg atcagggtcg
     3301 actccgccgg ccggcgccgc tacctcgatg ccgacttcgg aacgttcgcc gtcgaggttg
     3361 acggcggcct gcacctactt gcgcagaact attggtacga cgccaggcgc cagaacgacc
     3421 tcgtactggg cggagatcac atcttgcggt tcccaagcat cgcgatccga cttgaggaag
     3481 acgtcgtgct cgcccaactc cgccgcgctc accaggtatt cggctgactt ggaagctcga
     3541 cgacgccctc ttgagcgtct acagacttcc aagtcgcgca aacccccacg cgggacggcg
     3601 gttagctggc gactagcgga aggctgaccc gctgcgcacc ctctgcttgg gcgcgctgcg
     3661 caccctctgc ctgggcgcgc tgcgcaccgg gcgcggtcga cgcgaagtgc gacgccaccc
     3721 gctcgtccgg ctcgggatcg tcggccggtg tccagtgcac ccgcaggtgg ttgtacagcg
     3781 tgtcgcgctg cgctggcacc cggtcggccg accggatcag gtggatcagc tcggtcagat
     3841 tccagcggtg ccgagcaccc gccgacgaca cgacgttctc ctcgagcatc accgatccga
     3901 ggtcgtcggc gccgtagtgc agaccgagct gaccgacctc cttgccgagg gtgagctggg
     3961 atacctggag gtgggcgatg ttgtcgaaga agagtcgggc caccgcgagc agccgcaggt
     4021 actcaagcgg cgtcgccagt gtgcggccct tgaggtggtt gttctgcggt tggtaggtcc
     4081 acgggatgaa cgaccggaac ccgccggtgc ggtcttgcac gtcgcgaatc acgcgcagat
     4141 gttcgatgcg ttcggcgttc gtctcaccgg tgcccatcat gaacgtcgcg gtcgactgca
     4201 cgccgagccc gtgcgcggtc tccatgacct cgagccacac agccccggtt tccttcagcg
     4261 gagcgat
//
LOCUS       DASWVF010000206         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_285628, whole genome shotgun sequence.
ACCESSION   DASWVF010000206 DASWVF010000000
VERSION     DASWVF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285628"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VGD55_03690"
     CDS             complement(<1..660)
                     /locus_tag="VGD55_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0869479.1"
                     /translation="MSHHEGDDERIGVRKTYKLYIGGAFPRSESGRSYQVTTHGGEFA
                     ANAALASRKDARDAVVAARAAFPGWSRATAYNRGQVLYRIAEVMEGRRDQFVAEVQNT
                     EGISPDAAAAAVDAAVDRCVWYAGWTDKIAQVVGAANPVAGPFFDFSLPEPTGVVAIV
                     APPSSSLLGLVSVVLPVIATGNTAVVLASEQRPLPAITLTEVLATADLPGGVVNMLTG
                     RV"
     gene            complement(657..1871)
                     /locus_tag="VGD55_03695"
     CDS             complement(657..1871)
                     /locus_tag="VGD55_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0869480.1"
                     /translation="MPGRERSKYLFRIARIIQERSRELAVLESLDNGKPIKESRDVDL
                     PLVAAHFFYYAGWADKLEHAGFGANPRPIGVAGQVIPWNFPLMMLAWKIAPALACGNT
                     VVLKPAETTPLTALAFAEICQQAGLPPGVVNIITGAGETGRTLVGHDGVDKIAFTGST
                     EVGRAIAKTLAGTRKRLSLELGGKAANIVFDDAPIDEAVEGIVGGIFFNQGHVCCAGS
                     RLLVQESVADEVLDRLHRRMSTLRLGDPLDKNTDVGAINSAAQLARIRELSDIGEAEG
                     AERWSPACELPERGYWFPPTVFTGVTQAHRIAREEIFGPVLSVLTFRTPDEAIEKANN
                     TPYGLSAGVWTDKGSRILWMADRLRAGVVWANTFNKFDPSSPFGGYKESGFGREGGRH
                     GLEAYLAHGTSS"
     gene            complement(1781..2110)
                     /locus_tag="VGD55_03700"
     CDS             complement(1781..2110)
                     /locus_tag="VGD55_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0869481.1"
                     /translation="MTSKLFPYSPAPEARAIVNIAGSYGLFIEGEFVDSLDGEMFKTV
                     NPATEEVLAEVVDAGPRDVDRAVAAARKAYEGSWDRCPAGSVRSTSSASPASSRSARV
                     NSPCSSR"
     gene            complement(2119..3069)
                     /gene="deoC"
                     /locus_tag="VGD55_03705"
     CDS             complement(2119..3069)
                     /gene="deoC"
                     /locus_tag="VGD55_03705"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0869482.1"
                     /translation="MTLTTSAIPTDVASSNSGLRRFLHGLPGVDQVGAQARAAALSTR
                     SIKTDAKRWAIDTAISMIDLTTLEGQDTHGKVNALCAKGMRPDPGDSSCPSVAAICVY
                     PDLVATAKEHLRDSGVKVASVATSFPSGRAALSVKLADVRDAVSKGADEIDMVIDRGA
                     FLEGRYLDVYDEIVQVKAACKDAHLKVILETGELATYDNVKRASWLAMVAGGDFIKTS
                     TGKVAPAATLPVTLVMLQAVRDFRDTTGRQVGVKPAGGIRTTKDAMRYLVLVSETVGD
                     DWLSPDWFRLGASSLLNDLLLQRQKLASGHYSGPDYVTID"
     gene            3120..4109
                     /locus_tag="VGD55_03710"
     CDS             3120..4109
                     /locus_tag="VGD55_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0869483.1"
                     /translation="MKVLMLGGGTFVGRALVDAAVASGHEVTTFTRTTAPPAGVEELH
                     GDRTNPDGLDLVAAREWDVVFDTWSGAPRVVRQSVSVLRERAGYYGYVSTASVYDEPG
                     LGSDESHPTVDADPDADATDYPADKRGVELAILDGFGEQRCLLARAGIIVGPYESTGR
                     LVWWLRRIAAGGDVLAPGPASARLQWVDARDLAAWMLACATAGTTGAFTAISPPGFVG
                     FGEVLQECIDRTASGAQLVWVPPEFVAGQGFSEWVELPLWLPPGADFDSVYGLDTRRA
                     AAAGLACRPVAETIADTWAWMQTGGEPRTVAGRPRPGLDPEKERRALAAWASQ"
BASE COUNT          639 a         1339 c         1453 g          732 t
ORIGIN      
        1 tactcgcccg gtgagcatgt tcacgacgcc acccgggaga tccgccgtcg ctagcacttc
       61 ggtgagggtg atcgcgggca gcggccgttg ctcgctcgcg agcacgaccg cggtgttgcc
      121 cgtcgcgatg accggcaaca cgacagagac aagtcctagc aggctggacg acggcggcgc
      181 gacgattgcc accacgccgg tcggttcggg cagcgagaag tcgaagaacg ggccggcgac
      241 gggattcgcg gctccgacga cctgcgcaat cttgtcggtc caaccggcgt accagacgca
      301 acggtcgacg gcggcgtcga cggcggctgc tgcggcgtcc ggtgaaatgc cttctgtgtt
      361 ttgcacctcc gcgacgaatt gatcgcgccg accctccatg acctccgcga tgcggtagag
      421 cacctggccc cggttgtagg cggtcgcgcg cgaccagcct gggaaggctg cgcgcgcggc
      481 cacgactgca tcgcgcgcgt ccttgcgcga cgcgagcgcg gcgttcgcgg cgaactcgcc
      541 gccatgagtc gtcacctggt agctgcggcc cgattcactg cgcgggaacg cgccgccgat
      601 gtagagcttg taggtcttgc ggacgccgat gcgttcgtcg tcgccctcgt gatggctcat
      661 gacgacgtcc cgtgagcgag gtaggcctca agaccgtggc ggccgccctc gcgaccgaag
      721 cccgattcct tgtagccgcc gaacggcgat gacgggtcga acttgttgaa cgtgttcgcc
      781 cagacgacgc cggcgcgcaa gcggtcggcc atccagagaa tgcgactgcc cttgtcggtc
      841 cacactccgg ccgacaagcc gtacggggtg ttgttcgcct tctcgatggc ttcgtcaggc
      901 gtgcggaacg tgagcacaga gagcaccggc ccgaagatct cctcgcgcgc gatccggtgt
      961 gcctgggtta caccggtgaa gacggtcggc gggaaccagt acccgcgttc tggcagctcg
     1021 catgccggcg accagcgctc ggcaccctca gcttcgccga tatcggagag ttcgcggatg
     1081 cgagcgagct gagcggcgga gttgatcgcg ccgacgtcgg tgttcttgtc gagcggatcg
     1141 ccgaggcgca gcgtcgacat ccgccgatgc agccggtcga gtacctcgtc agcaaccgat
     1201 tcttgtacga gcaaccgcga gcccgcgcaa cagacatgac cctggttgaa gaagatgccg
     1261 ccgacgattc cttcgacggc ctcgtcgatc ggcgcgtcgt cgaagacgat gttggcggcc
     1321 ttgccgccaa gctcgaggct cagccgttta cgcgtgcccg caagggtttt cgcgattgcg
     1381 cggccgacct cggtcgatcc ggtgaacgcg atcttgtcga cgccgtcgtg cccgacgagc
     1441 gtgcgacccg tttcgccggc gcccgtaatg atgttcacga ctcccggtgg caggccagcc
     1501 tgctggcaga tctcggcaaa cgcgagcgcg gtgagcggcg tggtctcggc aggcttcaac
     1561 accaccgtgt tgccgcatgc gagtgcgggc gcgatcttcc acgccagcat catcagcggg
     1621 aagttccacg ggatcacttg gccggcgacg ccgatcggtc gtgggttggc gccgaagccg
     1681 gcgtgttcca gtttgtctgc ccagcccgcg tagtagaaga agtgtgccgc gacgagcggc
     1741 agatcgacgt cccgcgattc tttgatcggt ttgccgttgt ctagcgactc gagcacggcg
     1801 agttcacgcg agcgctcctg gatgatgcgg gcgatgcgga agaggtactt cgaacgctcc
     1861 ctgccgggca tcggtcccag ctgccttcgt aagccttgcg ggccgcggcg accgcgcggt
     1921 cgacatcgcg agggccggcg tcgacgactt cggcgagcac ctcttcggtt gcggggttga
     1981 cggtcttgaa catctccccg tcgagtgagt cgacgaactc gccctcgatg aacaggccgt
     2041 aggagcccgc gatgttgacg atcgcgcgcg cctcgggtgc cggcgaataa gggaacagct
     2101 tgctcgtcat gtgcgccttc agtcgatcgt cacgtagtcg ggaccggagt agtggccgct
     2161 cgccagcttc tggcgttgaa gcagcagatc gttgaggaga ctcgacgcgc cgagccggaa
     2221 ccagtcgggg ctcagccagt cgtcgccaac ggtctcgctc acgagcacga gatagcgcat
     2281 tgcgtccttg gtcgtgcgga tgccgcctgc cggcttgacg ccgacttggc gaccggtggt
     2341 gtcccgaaag tcgcgcactg cttggagcat gaccaaggtg acgggcagcg ttgccgccgg
     2401 tgcgaccttg ccagtactcg tcttgatgaa gtcgccaccg gcaaccatgg cgagccacga
     2461 cgcgcgcttg acgttgtcgt aggtcgcgag ctcgccggtc tcgagaatca ccttcaagtg
     2521 ggcgtccttg cacgcagcct tgacctgcac aatctcgtcg tacacgtcga ggtagcggcc
     2581 ctcgaggaat gcgccgcggt cgatgaccat gtcgatctcg tcggcacctt tcgaaaccgc
     2641 atcgcggacg tcggcaagct tgacgctcag tgccgcccgg cccgagggga agctagtcgc
     2701 gacgctggcg accttgaccc cgctgtcgcg caggtgctcc ttcgccgtcg caacgagatc
     2761 ggggtatacg cagatcgcgg cgacgctcgg gcagctgctg tcgcccggat cgggccgcat
     2821 gcccttggcg cacagcgcgt tcactttgcc gtgggtgtct tggccttcga gtgtggtcag
     2881 gtcgatcatc gagatcgccg tgtcgatcgc ccagcgcttg gcgtcggtct tgatcgacct
     2941 ggtcgacagg gctgctgcgc gcgcctgggc gccgacctgg tcgaccccgg gcaatccgtg
     3001 caggaatcgg cgcagcccag agttcgaact ggcgacgtcc gtggggatcg ccgacgtggt
     3061 gagcgtcatt catgcaccat atcgccggtc tgcgctgccc gggatgcgaa gctgggaggg
     3121 tgaaggtgct catgcttggt ggcgggacgt ttgtcgggcg ggcgctggtc gacgcggcag
     3181 tcgcgagtgg ccacgaggtg accacgttca cccggacgac ggccccgccc gccggcgtcg
     3241 aggagctcca cggtgaccgt acgaatccgg acggtctcga cctggtcgcg gcccgtgagt
     3301 gggacgtcgt gttcgacacg tggtcgggtg cgccacgcgt cgtccggcaa agtgtttcgg
     3361 tcttgcgcga acgggccggc tactacggct acgtgtcgac cgcctcggtg tacgacgagc
     3421 cgggtctcgg ttccgacgag tcacatccga ctgtcgacgc cgaccccgat gcggacgcga
     3481 ccgactatcc ggccgacaag cgcggcgtcg agctcgcgat cctcgacggg ttcggtgagc
     3541 agcgctgcct gctggcgcgc gctggcatca tcgtcggccc ctacgagagc accggtcggt
     3601 tggtgtggtg gctgcggcgc atcgccgccg gtggcgatgt gctcgcacct ggtccggcga
     3661 gtgcgcggtt gcaatgggtc gacgcccgcg atctcgccgc gtggatgctc gcgtgcgcaa
     3721 ctgcggggac caccggtgca ttcaccgcga tctcgccacc tggcttcgtc ggtttcggtg
     3781 aagtgcttca agaatgcatc gaccggacgg cgagcggtgc ccaactggtc tgggtgccgc
     3841 ccgaattcgt tgcggggcaa ggattctctg agtgggtcga gctgccactg tggctgccgc
     3901 ccggggctga tttcgattcg gtctacggcc tcgacacccg acgggcagcc gccgctgggc
     3961 tcgcgtgcag gccggttgcg gagacgattg ccgacacgtg ggcgtggatg cagacgggcg
     4021 gcgaaccgcg cacggtagcg ggccgaccac gaccagggct ggatccggag aaagagcggc
     4081 gcgcactcgc cgcctgggct tcgcagtaac cgcggcgagg cgcgccgcgg ctcgaatggg
     4141 ctgggctggg acgatgaact gcc
//
LOCUS       DASWVF010000207         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_158384, whole genome shotgun sequence.
ACCESSION   DASWVF010000207 DASWVF010000000
VERSION     DASWVF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158384"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="VGD55_03715"
     CDS             <1..1818
                     /locus_tag="VGD55_03715"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0869484.1"
                     /translation="TVPNHVALEISRLLPAFSAAASPPALTSAGLARLYSAVASTLAA
                     AAQGDPARPSIFVIDDAHWADDATVGVLGYLTNRLAELPIRIVLTWAPENAGRLTALR
                     TTVADAVAEGLAAVVHLEPFGLVEIAELLAAVPAGQSAPLDAGRLLTETRGLPLLVVE
                     YIRAAHGETVETPPAGVRDLLDRRIDAAHETTVQVVSAAAVLQGRFDADLLRAVSGRS
                     EAETADALDDAVRHRLLEENPATSDGEAPGYDFPFEALRRVVYERTTFARRRLLHGRA
                     ADALARRHERTLSTESAAAVSPAVVAHHFEQSGREAEAAAWWWRAAARSRGLYAHEQA
                     YSDLSRALALGYSPAAVWTSTGDVLVALGRYSEALSAYESAAGLAADAESAAGVEHKL
                     AEVHHRLGDWSAADDHLRTAVELLIAPDATPTLQEPDSSAALLARIGSDRALLAYRTG
                     DVAVARELADEALKTAHSAGDTAAAAQALDVLGMVAAAAGDLEAAESLLRRSIEQAAQ
                     LTDSGVAIAALNNLARVLADRGRRDEALDAAREALRLGTERGDQHRLAAPQTNLADLL
                     HAAGDETAALEHLKEAARLFAAVDTGGEPRAEVWTLVEW"
     gene            1916..3145
                     /locus_tag="VGD55_03720"
     CDS             1916..3145
                     /locus_tag="VGD55_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869485.1"
                     /translation="MTVQAGDALVGRPRLRWATSRTALWAGAVAVILLLAGAALSIAN
                     GGLLANVLSLIFLPFGVVGFIIARRQPRNPIGWILLAILIAIAFAGAAGQYAVLYFSG
                     GHHALPLARVAVFAAAGWIWMVVLMPLPIGLFPDGRLSRRWRIVLWIYLIDIGALLTV
                     FGIVDLTGVLAHHLTLDSSGELKTFGGSQTGWASTAEAFLIPIYVGVALSWVVRQVVA
                     YRGSTGDRREQLKWLISGGLICIVGLIVSLQLGPSHSTLWHWVANLSSLGIIALPVSL
                     GIGILKYRLYEIDRLISRTVSYAIVTGLLVGVYVGMIALTTRLIPLSSSVGVAASTLV
                     AVGLFTPVRRSVQRRVDRRFNRARYDAEAMVATFTTRLRDAVQLETVRGDLLDVVSAA
                     VEPSQVSLWIRPVSRVP"
     gene            3142..>3596
                     /locus_tag="VGD55_03725"
     CDS             3142..>3596
                     /locus_tag="VGD55_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869486.1"
                     /translation="MMARWVTRFATSRTALVFGVVLLVFLAAAVPLSLLTPNLSESVP
                     FLPAAIIGVILARQRPGNAIGWVFVALAMTVFITGDAAQYSLLAYRLHHPGLPLARLA
                     LALNALWIGFILLLPLPILLFPDGRVAGRFWRWTLRVYLAMSAIVFLILG"
BASE COUNT          498 a         1154 c         1240 g          704 t
ORIGIN      
        1 acggttccga accacgtcgc gctcgagata tctcgtttgt tgccagcgtt ttcggccgca
       61 gcttcgccgc ccgcattgac gagcgccggg ctcgcgcggc tctacagcgc cgtcgctagc
      121 acgttggcgg cggccgccca gggagatcca gctcgaccgt cgatctttgt catcgatgac
      181 gcgcactggg ccgacgatgc gaccgtcggc gtgctcggct acctgacgaa tcggctggcg
      241 gagctgccga tccgcatcgt cttgacgtgg gcgcccgaga acgccggacg cttgactgcg
      301 cttcggacca cggtcgccga cgcggtggca gaagggctcg ccgccgtcgt ccatctcgag
      361 cctttcgggc ttgtcgaaat cgcggagctt cttgctgctg tgccggccgg gcagtcggca
      421 ccacttgatg ccggccgcct gctgactgag acccgtgggc tgccgctgct cgttgtcgag
      481 tacatcaggg cggctcacgg cgaaacggta gagacgccgc cagcgggcgt gcgcgacctg
      541 ctcgaccggc gcatcgacgc ggcacatgag acgacggtgc aggtcgtgtc cgcggcggcg
      601 gtgttgcaag gccggtttga cgctgacttg ctgcgcgcgg tgagcggtcg cagcgaggcc
      661 gagacagccg acgcgttgga cgacgcggtc aggcatcggc tgctggaaga gaatccagca
      721 acgtcggacg gcgaggcgcc ggggtatgac tttcctttcg aggcattgcg gcgggtcgtc
      781 tacgaaagga ccacgttcgc ccgacgtcgt ctgctgcacg gccgcgcggc tgatgcactc
      841 gcgcgccggc acgagcgcac gctttcgacg gagtcggcag ccgcagtttc accggccgtc
      901 gttgcgcatc acttcgagca atcggggcgc gaagcggaag ccgccgcctg gtggtggcgg
      961 gccgctgcca ggtctcgcgg gctgtatgcc cacgagcagg cgtactcaga tctctcccgg
     1021 gcgttggcct tggggtattc acccgccgcg gtatggacga gcaccggcga tgtccttgtc
     1081 gcgctgggtc ggtactccga ggcgctgagt gcttatgaaa gcgcggccgg cctcgcagct
     1141 gacgccgagt ctgccgcagg ggtcgaacac aaactcgccg aggtgcacca ccgcttgggt
     1201 gactggtcgg cagccgacga tcacttgcgc accgcagtcg agttgcttat tgcccccgac
     1261 gccaccccga cccttcagga acccgactcg tcggcggcgc tgctcgcgag gatcggctca
     1321 gaccgcgctt tgctcgccta ccgaacgggc gacgtggcgg tcgctcgcga actcgccgac
     1381 gaggcgctca agacggcgca ctctgcgggc gacacggcgg cggcagccca agcactcgat
     1441 gtgctgggca tggtggccgc ggccgccggc gatctagagg cagccgaatc gctgttacgg
     1501 cgaagcatcg aacaggcggc gcagttgacc gactccggtg tcgcgatcgc ggcgctgaac
     1561 aacctcgcgc gcgtgctggc tgatcgcggg cggcgcgacg aggccctcga cgcggctcgc
     1621 gaggccttgc ggctcggcac ggagcgcggc gaccagcacc ggctcgcggc cccgcagaca
     1681 aatctcgccg acttgctgca tgcggccggt gacgagacgg cggcccttga gcacctcaag
     1741 gaagcagcgc gcctcttcgc ggcagtcgac accggcggcg agccgcgggc agaggtatgg
     1801 acgcttgtcg agtggtgatg ggcgggtgat gtgccgtaac gcttcgggaa cgctcgacac
     1861 ttaggtttgc cccagatctg cctcggccca acggggcgcg ggagaaggag tgcgggtgac
     1921 ggtgcaggct ggcgatgccc ttgtgggtcg tccgcgtttg cgatgggcga cgtcgcgtac
     1981 ggccttgtgg gcgggggcag tcgcggtgat tcttctgctg gcaggggcgg cgctgtcgat
     2041 cgccaatggc ggcctcctgg cgaacgtcct ctcgttgatc ttcctgccgt tcggtgtcgt
     2101 cggcttcatc atcgcccggc gccagccacg gaatccgatc ggctggatcc tgctcgcgat
     2161 cctgatcgcg atcgcgttcg caggcgcggc tgggcagtac gcagtgctct acttcagcgg
     2221 gggccatcac gccctgccgc tcgctcgggt ggcagtgttc gcagccgcgg gttggatctg
     2281 gatggtggtg ctcatgccgc tgccgatcgg gctcttcccc gatggtcggc tctcacggcg
     2341 gtggcggatc gtgttgtgga tctacctcat cgacatcggt gctctgctga cggtgttcgg
     2401 gatcgtcgac ctcaccggtg tgctcgccca ccacctcaca ctcgattcat ccggagagct
     2461 aaaaaccttc ggcgggagcc agaccggctg ggcgtcaacc gcggaagctt tcctcatacc
     2521 catctacgtg ggcgtggcac tgtcgtgggt ggtccgtcaa gtggtggcgt accgcggttc
     2581 taccggcgac cgacgtgagc agctgaaatg gttgatcagc gggggcctga tctgcatcgt
     2641 cggcctcatc gtctcgcttc agctcggccc tagccattcg acgttgtggc actgggtggc
     2701 gaacctgagt tcactcggca tcatcgcgtt gccggtgtcg ctcggcatcg ggatcttgaa
     2761 gtaccggttg tatgagatcg accggttgat cagcagaacg gtctcgtacg caatcgtcac
     2821 tggacttctc gtgggcgtct acgtcgggat gatcgcgctg acgacgcggt tgatcccgct
     2881 gtcgtcgtcg gtcggcgtgg ccgcatcgac ccttgtcgcg gtcgggctct tcacgccagt
     2941 gcgccgcagc gtgcagcgcc gtgtggatcg gcggttcaac cgagcccgct atgacgccga
     3001 ggcgatggtg gccacgttca cgactcggct gcgcgacgcc gtccaacttg aaaccgtgcg
     3061 cggcgatctg ctggatgtcg tgagtgcggc cgtcgaaccg agccaggtgt cgttgtggat
     3121 ccgacctgtg agccgggttc catgatggcg cggtgggtca ctcgattcgc gacgtcgcgg
     3181 accgcgctcg tcttcggtgt tgtactccta gtgtttcttg ccgcagctgt gccgctttcg
     3241 ctgctcaccc ccaacctctc tgaatccgtg ccgttcctgc cggcggcgat catcggcgtc
     3301 attctggcgc ggcagcgtcc gggcaatgcg atcggctggg ttttcgttgc gttggcaatg
     3361 acggtcttca tcactggcga tgcagcgcag tacagcttgt tggcttatcg gctgcatcat
     3421 ccaggccttc ccctcgcccg cctcgcactc gcgctcaacg cgctctggat cgggtttatt
     3481 ctgctcttgc cgctgccgat cttgctcttc cccgatggtc gggtcgctgg gcgtttctgg
     3541 cggtggacgt tgcgggtcta cctggccatg agtgcgatcg tgttcctcat tctcgg
//
LOCUS       DASWVF010000208        19423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_209614, whole genome shotgun sequence.
ACCESSION   DASWVF010000208 DASWVF010000000
VERSION     DASWVF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 19423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19423
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209614"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGD55_03730"
     CDS             complement(<1..272)
                     /locus_tag="VGD55_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0869487.1"
                     /translation="MADSNNTFELIVAGLDTFGAKVTAITPDRWLRATPCTDWSVRDV
                     VNHVTSEHLWVPHLLRGETIAQVGSRYDGDVLGDDPVYAWERASALS"
     gene            complement(306..1013)
                     /locus_tag="VGD55_03735"
     CDS             complement(306..1013)
                     /locus_tag="VGD55_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY0869488.1"
                     /translation="MTGVTPEPAVPGPLVAVSPHLDDVVLSCAGLIASVPGATVITVF
                     AGYPPGRPESLSENITSWDAASGFALGDDVVAIRRDEDRAALAHLGAVPLWLDFLDSQ
                     YTTQPATVPVIAAGIASSLAGLSVETIAFPLGLSHTDHERTHDACIWLLEEFPALATN
                     WVAFIDVPYRARFRPQAVQRLEHLGELGYDLEPLSFDLGDRKVAALAEYPSQLKALAA
                     DIGNAALPEECYLLRHR"
     gene            complement(1092..1174)
                     /locus_tag="VGD55_03740"
     tRNA            complement(1092..1174)
                     /locus_tag="VGD55_03740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1139..1141),aa:Leu,seq:cag)
     gene            1293..2195
                     /locus_tag="VGD55_03745"
     CDS             1293..2195
                     /locus_tag="VGD55_03745"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEY0869489.1"
                     /translation="MGVLSRFERKLGGMVEGVFARAFKSEVQPVEVAAALQRELDDQA
                     AVVGRDRTLVPNHFVVELGDHDHDRLAPYEKPLTDELAAMVREHADDQRYSFVGPVRV
                     ELERVHPLDTGVFRIRSDVAPGREGLRDLAAPAQPLRAPARPAAAPLGVRPEALFELG
                     REPDDTNLTTAIPRSVATPNLGRIQVREGKAASYDFELKHKITVIGRGTDADIRLTDH
                     AVSRRHAEIRIANGATMLNDLQSTNGTTVNGVTVTTTALSDGDEIRIGETVLTYRAPA
                     ASASPGAPSLPGLPAVPDNQGQWG"
     gene            2205..2663
                     /locus_tag="VGD55_03750"
     CDS             2205..2663
                     /locus_tag="VGD55_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0869490.1"
                     /translation="MSELSLFLLRAGFVALLWVFVIVAFGIVRSDLSGGAQPRVVRPQ
                     PKKQAKRPSSRKAPRKLVVVAGALAGTSINLGDAPVTIGRANDSTLVLTDDYASTKHA
                     RVFMRDGQWLVEDLGSTNGTFLDRAKVTQPTPVGTGTPIRIGKTVLELRR"
     gene            2671..3957
                     /locus_tag="VGD55_03755"
     CDS             2671..3957
                     /locus_tag="VGD55_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY0869491.1"
                     /translation="MTLMLRFAARSDVGLIRTNNEDAVYAGPRLVAVADGMGGHAAGD
                     VASAVTIATLAPLDDDDPPSDLLGALEESIRQANDQLRQMMDSDAELDGMGTTLTALL
                     WGGTRLALAHIGDSRAYLLRDGELTQITQDHTLVQHLVDQGQLDEADIPTHPQRSVIL
                     RVLNGRPDAEADLSIREARAGDRYLLCSDGLSGVVSKDTLRQALAIADPEQVVDALVD
                     LALRGGAPDNVTCVVCDVVEAGTATAGTPVIGGSVADGDGDRVGEPDTAAGRAAALRI
                     HRRPAATRATHEEPVRRRWRGPALVVVLLVVVAGALFGGTWWYSQHQYYVGSNGAQVV
                     VYRGINGTVLGLNFSSPIENTGIVMSSLPAFERDRVNDTIGARNKTDADRIVRQLHPS
                     PSPVSSPGAQPSAPASAAPGSSAPPSSAPSSTAGSP"
     gene            3954..5360
                     /locus_tag="VGD55_03760"
     CDS             3954..5360
                     /locus_tag="VGD55_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0869492.1"
                     /translation="MTTAAATGAGGLVRAIKPRRPRTRRSVELAMLVFAVIIALAAYA
                     DVGLTRSQRVPPDILTYGLGLGALVLLAHIAVRRFAPYADPLLLPCAALLNGLGLVLI
                     HRLDLVAPKAARSDATLQLAWSAIGVVGFIAVLVIVRDHRRLQRFTYTSMLLGLVLLA
                     IPSILPARFSEVNGARNWIRFAGFSIQPGEFAKVLLVIFVAGYLVAKRDALALAGHRF
                     AGIDLPRGRDLGPVVLAWLVSVGLLVRGRDLGMSMLFFGFFILMLYIATERLSWLLIG
                     TLLFIGGTYVAYHLFAHVRERFEIWLHPFAHAQGSGYQLVQGLYGFATGGLMGTGLGN
                     GRPDLVPFANSDFIMATIGEELGLTGLMAILLVYVVIVSRGLRAALSVRDSFGKLLAA
                     GLSVCLAVQVFVVVGGVMRLIPLTGITTPFLSYGGSSLVSNWILLAVLLRISDAGRRP
                     VAAPTPRPDDAMTQVISL"
     gene            5357..6808
                     /locus_tag="VGD55_03765"
     CDS             5357..6808
                     /locus_tag="VGD55_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0869493.1"
                     /translation="MNKPIRRVAITCLLLFAILLVNDNVVQFADAKTLRDKPGNTRLL
                     FQQYDRKRGDIVTSTGVTIASSVPTTDALKYLRTYPNGPLFAPVTGYYSIVYGASQIE
                     SSENSILAGSDDRLFGVQQLSNLITGRTPEGGSVVLTIDPVLQQVAYTALAGRKGAVV
                     ALDPTTGAILALVSSPSYDPNTLSSHDQTTITAYWKKLIADTANPGLDRALNQTYPPG
                     STFKLITAAAALSTGSYTPSTVIPAPATLPLPQSTSVLHNFQGESCGGQATLIQALTI
                     SCNTAFGGLGLALGQDALRTQADAFGFDSPYTVPMKSATSVFPDDLSDANTIQSAIGQ
                     FDVRATPLQMAMVAAAIANGGVEMKPYLVDQLVGPDLRIVDQTKPAPLRRAVSAQVAA
                     ELTLMMESVVQHGTGTSAQIPGVAVAGKTGTAQNAIGAAPHAWFVAFAPAVQPKIAIA
                     VLVENGGGNSDATGGAIAAPIAKAVIQAELKKK"
     gene            6917..8629
                     /gene="pknB"
                     /locus_tag="VGD55_03770"
     CDS             6917..8629
                     /gene="pknB"
                     /locus_tag="VGD55_03770"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY0869494.1"
                     /translation="MTEPRRLGDRYELGEVLGYGGMAEVLRGRDIRLGRDVAVKTLRV
                     DLAREPSFQARFRREAQSAASLNHPAIVAVYDTGESLLDGVPVPYIVMEYVDGRTLRD
                     VLKAERHIMPTRALEITAQILNALDYSHRNGIIHRDIKPANVMLTRSGEVKVMDFGIA
                     RSVAQASAAVTQTAAVIGTAQYLSPEQARGEQVDPRSDVYSTGCLLYELLTGTPPFTG
                     DSAVAVAYQHVREDPIPPSRVNPDVSPAIDAIVLKAMAKNPANRYQSAAEMREDIERA
                     LSGVPVQATPLLHDQTVSIAPIVEDEYVRRQPRGRRSLTYIMVILIAIGMFILIGVIA
                     RHLLTTKHQAVTVPVPNVSGFSQSDAIASLKSAGLNPVVLPGTSATVAKGIVISENPP
                     QGTPLPLKTNVTIYVSTGPQAVQVPDLTNQSLTDAKSELLRNKLILGTTTTKDSPQSA
                     NTVLSQTPAAGANVTSGSAVNVVVASGNSTVPDVRNLPVAEAESALTSFGFNFQEIDQ
                     PSSTAAPGIVINQSPPHDSPAKTGTIVKLFVSVAPPPSSSPPANSPPPTATPTNTATT
                     SPSA"
     gene            complement(8630..9241)
                     /locus_tag="VGD55_03775"
     CDS             complement(8630..9241)
                     /locus_tag="VGD55_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0869495.1"
                     /translation="MRILVVDNYDSFVFNLVQYLGQLDATCVVLRNDQLSPSDALDFD
                     GVLLSPGPGTPEDAGVCVPLIREVAGRVPIFGVCLGHQAIAVAYGAVVNRAPELLHGK
                     TSAVHHNGAGVLAGLPDPFTATRYHSLAVVESTLPTCLEVTARTDSGVVMALWHRDVD
                     VEGVQFHPESVLTQGGHQMLANWLVRCGDADAPARAGLLSSPL"
     gene            complement(9282..9533)
                     /locus_tag="VGD55_03780"
     CDS             complement(9282..9533)
                     /locus_tag="VGD55_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869496.1"
                     /translation="MYGWIWRRLPGGFGAKVGGCALLLVGVLALLFFVIFPWAEPRLP
                     WNDVTVNSPSVEETVPSSSPPALPPPSALPGGPSVLPAG"
     gene            complement(9536..10303)
                     /locus_tag="VGD55_03785"
     CDS             complement(9536..10303)
                     /locus_tag="VGD55_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEY0869497.1"
                     /translation="MTTTREFALVTRTSPLNQVIRLIGELLITLGVIVFLFIPYELWF
                     TGLYTEHAQAQIKRDLAASWASPAPVASASPGATASGPSPSSVPTASAPTVAPVTLIA
                     NAIGIIRIPRLGANYGYAIVQGVSTDDLKKGPGHYPGTADPGGLGNFVISGHRTTYLA
                     PFNGLGNLAQGDAIVIETRDTYYTYRVTTIQTVLPTDVGVILPVPGQPRATPTQALIT
                     LTTCTPKYSASHRLVVTGLLESALPKAAGLPPALKGA"
     gene            complement(10300..11055)
                     /locus_tag="VGD55_03790"
     CDS             complement(10300..11055)
                     /locus_tag="VGD55_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY0869498.1"
                     /translation="MRAAVAASTRAARAWRYGVPIAALLAGLMFAASATLSRGFDLRV
                     TGRDDVNSLVNRQQRTVITQGAQLAALQRKLQAETSQAAKTDSRVAQARDEADAFANA
                     AGLEPVTGPAVSVTLNDAPALPGKQRTAVPPDYLVVHQQDVQAVVNALWAGGAEAMTL
                     QGQRVISTSAVRCVGNTLLLHGVVYSPPYTVEAIGDPQKLQQALDHAPDVIIYKQYVD
                     AYQLGYKLALEGDVTMPAFTGPLTLAYARAVTS"
     gene            11141..11398
                     /gene="crgA"
                     /locus_tag="VGD55_03795"
     CDS             11141..11398
                     /gene="crgA"
                     /locus_tag="VGD55_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY0869499.1"
                     /translation="MPKSRVRKTEVFTPPPRRSANKRVSPPWLAPTMVGFMLVGVLWL
                     VLGYVTNYAFPGMSTLGSQGGNLIEGFALLVVGLGLATQWR"
     gene            complement(11563..12285)
                     /locus_tag="VGD55_03800"
     CDS             complement(11563..12285)
                     /locus_tag="VGD55_03800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0869500.1"
                     /translation="MRQGRTPLGGRPSANPHAVTYALIGLNVLAYLGEVASTAFVQRF
                     WQFGATGFTANGKVHVVAGVAHGEYYRLLTSMFLHDPPSSGGLFILHILFNMYALYIV
                     GPPLEALLGRVRFAAMYLLTGLAGSVLAYVLTAPNVPELGASGAIFGLFGALLVVGRK
                     LRLNIQPIAVTIGLNLVLTFSLAGISWQAHIGGLVAGAALAAAWAYAPRPSRTAIQIG
                     SSIALIAVMVVAVVLRTHNLTG"
     gene            complement(12472..12996)
                     /locus_tag="VGD55_03805"
     CDS             complement(12472..12996)
                     /locus_tag="VGD55_03805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0869501.1"
                     /translation="MADETFATFHTTKGDIEVRLFPNHAPKTVANFVELAEGGKEWKD
                     PRSGGKTTAKLYDGTIFHRVIEGFMIQGGDPLGSGRGGPGYQFTDEFHPELAFNRPYL
                     LAMANAGPNTNGSQFFITVAPTPHLNRKHTIFGEVTKGSEVVDAIATAPAGAGDKPRT
                     DIVINSITVERRTV"
     gene            13133..13621
                     /locus_tag="VGD55_03810"
     CDS             13133..13621
                     /locus_tag="VGD55_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869502.1"
                     /translation="MFTRTDKMTSKLNDTVTARVAAARQLLDAKVIPYAVPAGRKAAK
                     QVRQVVGERIVPVVDNARVASAPARAEAFRRGRLAAAALRGGGTMIAKKRRRWPVALV
                     CLGLGGALGAGAAWLAQAGKPVQLTPYPLDDDTDAGHDHEHHSLNGSHPLDLTDESHA
                     HH"
     gene            complement(13668..14699)
                     /locus_tag="VGD55_03815"
     CDS             complement(13668..14699)
                     /locus_tag="VGD55_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0869503.1"
                     /translation="MFERFSAQARQVVVLAQEESRRLSHNYIGTEHLLLGLLAQPEGV
                     AKQALDASGMTLDGARGSVEAAVGKGKRKPLGHIPFTPRAKKVLELSLREAVHLRADH
                     IGTEHLLLGLIREGDGLGARVLTESAGDLLAVRLKVLDLVPPAGPSESRRRLFWQRSR
                     NSAEVEAAAAIQIRTTGATDATLDEAQRLAGEAAVGSHHLLLATLNDPQSAAAKALGT
                     LGVDLEQARTALRSVDLVGTTDELPEEAGRRQLAVHLRGAVLTLETTDEALVSLARSA
                     LESLQVIAMLEAPGVTDDGTNDEIRGGDPRTRGFADLWQAWHLSLQATVDSLQATKDA
                     ASASAHEPG"
     gene            14780..15151
                     /locus_tag="VGD55_03820"
     CDS             14780..15151
                     /locus_tag="VGD55_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869504.1"
                     /translation="MEPIDWNDRLDAWDAALELSAQLDGSEWVTLDVAARQTGASRAA
                     LRTWYRNEEIPSRLTEGPHGPTRLVPLTVVAARAEASPRLRYSAQRRLDAEAQVALLR
                     HRVDVLEGRLSALESRLTAAQ"
     gene            complement(15170..15337)
                     /locus_tag="VGD55_03825"
     CDS             complement(15170..15337)
                     /locus_tag="VGD55_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869505.1"
                     /translation="MSPLWSNADPEPIGTGAKLDTLIAGGRWIVLEQAVAATTIRPAA
                     IDNLNDISRLL"
     gene            15318..15656
                     /locus_tag="VGD55_03830"
     CDS             15318..15656
                     /locus_tag="VGD55_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869506.1"
                     /translation="MDQSGDTGTQAGQLASQLGCTDFSADVQLDRYAQDEATCLINGD
                     NEVTIFDFSGQADEQAWFALGPESADGSGTVVLGGGWAVEPSNAGQAAFIQQTLGGTI
                     SQQQGLGGSS"
     gene            complement(15689..15761)
                     /locus_tag="VGD55_03835"
     tRNA            complement(15689..15761)
                     /locus_tag="VGD55_03835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15726..15728),aa:Ala,seq:tgc)
     gene            complement(15794..15928)
                     /locus_tag="VGD55_03840"
     CDS             complement(15794..15928)
                     /locus_tag="VGD55_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003999697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HEY0869507.1"
                     /translation="MKKILLLAVVLAGGLVIYRRVQAEQAEEDLWIEATSAVEPEPVA
                     "
     gene            complement(15958..16031)
                     /locus_tag="VGD55_03845"
     tRNA            complement(15958..16031)
                     /locus_tag="VGD55_03845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15995..15997),aa:Ile,seq:gat)
     gene            complement(16090..16773)
                     /locus_tag="VGD55_03850"
     CDS             complement(16090..16773)
                     /locus_tag="VGD55_03850"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEY0869508.1"
                     /translation="MSDSDLGGRPARSPYARPDPVATAEPPAALVESVPDRPADTDEH
                     GPATVLSAPVPAASAPTGWGGAAAAGSMATKPADSGGLVGSGGDGGLAAPVRSKAVQV
                     AVARRGPRRARLVIKHIDPWTVLKISFVVSIVMLAVIVIAVAVIYAVLGRMGVWTQIN
                     TLVNEVSPTTTSSTLHNPVSAGRVVGVAAVIGAINVVLLTALSTLGAAIYNLISDLVG
                     GVEVTLTDP"
     gene            complement(16770..19421)
                     /gene="gyrA"
                     /locus_tag="VGD55_03855"
     CDS             complement(16770..19421)
                     /gene="gyrA"
                     /locus_tag="VGD55_03855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0869509.1"
                     /translation="MEPVDLQVELQRSYRDYAMSVIVGRALPDVRDGLKPVHRRVLYA
                     MYDGGYRPDRGFNKCSRVVGDVMGQYHPHGDSSIYDALVRLAQPWSLRYPLVDGQGNF
                     GSPGNDPPAAMRYTECRMAQLAMRMVDDIDEETVDFTANYDGKTMEPLLLPSRFPNLL
                     VNGSAGIAVGMATNIPPHNLREVAAGAQWALEHADATREELLEALLERIAGPDFPTGG
                     LIVGRRGIEDAYRTGRGSVTMRAVIDVEEDTRGRQLLVITQLPYQVNPDNLALRIAEL
                     VNEGKLAGIADIRDDSSSRTGQRLVIVLKRDAVAKVVLNNLYKHTQLQDTFGCNMVAL
                     VDGVPRTLALDGFIRHWIAHQIEVIVRRTRYRLRKKEERAHIVRAYLAALERLDEVIA
                     LIRNSATVEVAREGLMALLAIDETQANAILDMQLRRLAALERQKLIDEYEELSADIAD
                     LNAILVNEPRQRTIVRDELAEIVEKHGDERRSHIIAAEGEVSMEDLIAEEDVVVTISN
                     GGYAKRTKTDLYRAQRRGGKGVIGAQLRQDDVVEHFFVTTTHHWLLFFTDKGRVYRAK
                     AYELPEAARSARGQHVANLLAFQPDETIAQVLALRDYEVAPYLVLATREGLVKKTALR
                     EFDSNRSGGVIAINLRDDDAVIAARLVSASDDLLLISRKGQSIRFHANDEALRPMGRA
                     TSGVIGMRFRPGDQLLAMMVARGDSFVFTATDGGFAKRTPIEEYPVQGRGGQGVLTAK
                     LTDDRGGLVGALIVDPGDEIFAITSGGGVIRTRSDEVRPTSRATMGVRLINLGDGDAV
                     VGITRNAEAASEVADAGELDGELDADLDTELDTEASGAAEESGGHNGVDGKMTAPPPA
                     PASTGTDMVDSQGPDGLGAFDDDQGDS"
BASE COUNT         3395 a         6472 c         6234 g         3322 t
ORIGIN      
        1 gacaacgcgc tcgcgcgttc ccacgcatag accggatcgt cgccaagcac gtcgccgtcg
       61 tagcgcgagc cgacctgcgc gatggtctcg ccacgcagca agtggggtac ccacaggtgc
      121 tcgcttgtca cgtgattcac cacgtcgcgc actgaccaat cggtgcacgg cgtcgcgcgc
      181 aaccaccggt cgggggtgat cgccgtcacc ttggcaccga acgtgtcgag gccggccacg
      241 atgagttcga aggtgttgtt cgagtccgcc atgatcggca ccgtagacct cgggcgttcg
      301 tctgactagc ggtgccgaag caggtaacac tcctccggca gagcggcgtt tcctatgtcg
      361 gccgcaagcg ctttgagttg gctcggatac tccgccaacg ctgcgacttt gcggtcaccg
      421 aggtcgaacg acagcggctc gaggtcgtac ccgagctcgc cgaggtgctc aaggcgttgc
      481 acggcttgcg ggcggaagcg ggcccgatac gggacgtcga tgaacgccac ccagttcgtc
      541 gcgagggccg ggaattcctc gagcagccag atgcacgcat cgtgagtgcg ctcgtgatcg
      601 gtgtgcgaca acccgagcgg gaacgcgatc gtttcaaccg acagcccggc aagcgatgac
      661 gcgatacccg cagcaatgac tggcacggtg gcgggctgcg tcgtgtactg gctgtcgaga
      721 aaatcaagcc atagcgggac ggcgccgagg tgggccagcg ccgcacggtc ctcgtcgcga
      781 cggatcgcca cgacatcgtc gcccagcgcg aatccggagg ccgcgtccca ggacgtgatg
      841 ttctcgctca gtgactcagg acgccccggt gggtagccgg caaagaccgt gatcacggtc
      901 gcgccaggaa ccgacgcgat caggcccgcg cagctgagca ccacgtcgtc gaggtgcgga
      961 gacacggcaa cgagggggcc tggcacagcg ggttcaggcg tgacgccggt cacaggttcg
     1021 cccctgtcac tagaggtcgt tgaaagcttg aggggctcac gtgcgggtca tcatggattg
     1081 gcgttcggga gtgggcgagg ggggacttga acccccacgt cctcacggac acacggacct
     1141 gaaccgtgcg cgtctgccat tccgccactc gcccgagtgg agcgctcagc gtagcagggg
     1201 cggccgcgaa ccaggactgg tcgccaagcg tcctagatac gatcaatgag gcacgaacgg
     1261 ccaccagacg acgtcggacg gaaggagggt cagtgggcgt gctttcgcgc ttcgagcgca
     1321 agctcggcgg aatggtcgaa ggagtcttcg cccgcgcctt caaatccgag gtccaaccag
     1381 tcgaggtcgc ggccgctttg caacgcgaac tcgacgacca ggccgccgtc gtgggccgcg
     1441 accgcacgtt ggtgccgaac cacttcgtcg tcgaactcgg cgaccacgac cacgaccggc
     1501 tcgcgccgta tgagaagcca ctgaccgacg agctggccgc gatggtccgt gagcatgccg
     1561 atgaccagcg ctacagcttt gtcggaccgg tgcgcgtcga gctcgagcga gtacacccac
     1621 tcgacactgg cgtgttccgc atccgcagcg acgtggcgcc agggcgcgaa ggcctgcgtg
     1681 acctggcggc tccagcccag ccactacgag cgcccgcgcg accagcggcg gcaccgctcg
     1741 gcgtccggcc agaagcactt tttgagcttg gccgcgagcc cgacgacacc aacctgacca
     1801 ccgcgatccc gcgatccgtt gccaccccga acctcgggcg gattcaggtg cgcgaaggca
     1861 aggcggcgtc gtacgacttc gagctgaagc acaagatcac cgtcatcggt cgtggcaccg
     1921 acgctgacat caggctcacc gatcacgccg tctcgcgccg tcacgccgag atccgcatcg
     1981 ccaacggcgc gacgatgctg aacgacctgc aatcgaccaa cggcacgacg gtcaacggcg
     2041 tcaccgtcac gacaacggct ttgtcggacg gcgatgagat ccgtatcggc gaaacggtgc
     2101 tgacctaccg cgcaccggct gcctctgcgt ctccgggcgc gcccagcctg ccaggcctgc
     2161 cggccgtccc cgacaaccag ggccagtggg gctgagcgcc gccgatgtca gaactctcgc
     2221 tcttcttgct gcgagcggga ttcgtcgccc tgctgtgggt attcgtcatc gtggccttcg
     2281 gcattgtgcg ctcagacctg tcgggcggcg cccagcctcg cgtcgtgcga ccgcagccga
     2341 agaagcaggc gaagagaccg agctcgcgga aggcgccgcg caagctcgtc gtcgtagcgg
     2401 gtgcgctggc cgggacgtcg atcaacctcg gcgatgctcc ggtgacgatc gggcgggcga
     2461 acgactcgac cctcgtgctg acagacgact acgcctcgac caaacacgcg cgggtcttca
     2521 tgcgggacgg ccagtggttg gtcgaagacc tcgggtcgac caacggcacg ttcctcgatc
     2581 gcgcaaaagt cacccaaccg acgccggtcg gcaccggcac gccgatccgg atcggcaaga
     2641 ccgttctgga gttgcgccgg tgatcgccgc atgacgctga tgctgcggtt tgcagcgagg
     2701 tctgacgtcg ggctcatcag gaccaacaac gaggacgccg tgtatgccgg gcctcgtctt
     2761 gtcgccgtcg ccgatggcat gggcgggcac gcggctggcg atgtcgcgag cgcggtcacc
     2821 atcgcgactc tcgcgccact cgacgacgat gacccaccgt cggatctgct cggtgcgctc
     2881 gaagaatcca tcaggcaagc caacgaccag ctgcggcaga tgatggattc cgacgccgag
     2941 ctcgacggca tgggcaccac gttgacggca ttgctgtggg gcggcactcg gctggcgttg
     3001 gcgcacatag gagactcacg ggcctacctg ctgcgcgacg gtgagctcac ccagatcacc
     3061 caagaccaca cgctcgtgca gcacctcgtc gatcagggcc agctcgacga ggccgacatc
     3121 cctacgcacc cgcaacgctc ggtgatcctg cgcgtgctca acggccgccc tgacgccgaa
     3181 gccgacctgt cgattcgcga ggcgcgagca ggcgatcgtt acctgttgtg cagcgacggc
     3241 ctctcgggcg tggtgagcaa ggacacgttg cggcaggccc tggcgatcgc cgacccggag
     3301 caggtcgtcg acgcgctggt cgacctcgcg cttcgcggcg gggctcctga caacgtgacg
     3361 tgcgtcgtgt gcgacgtcgt cgaggcaggc accgcgaccg cggggacgcc ggtgattggt
     3421 ggatccgttg ccgacggtga cggcgaccgc gtcggtgaac ccgacacggc ggccggccgc
     3481 gctgccgcac tgcgtatcca tcgacggccc gccgcgaccc gcgcaactca cgaagagccg
     3541 gttcgtcgcc gctggcgagg gccagcgctc gtcgtcgtcc tgctcgtcgt cgttgctggt
     3601 gcgttgtttg gcggcacctg gtggtattcc cagcatcagt actacgtggg cagcaacgga
     3661 gcgcaggttg tcgtctatcg gggcatcaac ggcaccgtgc tcggcttgaa cttttcctca
     3721 cctatcgaaa acaccggcat cgtgatgtcg tcgttgcccg cgttcgaacg cgaccgcgtc
     3781 aacgacacca tcggcgcacg caacaagact gacgccgatc gcatcgtgcg ccaactgcac
     3841 ccgtctccgt cacccgtgtc atcacccggt gcgcagccgt ctgcgccggc ttcggcagcc
     3901 ccagggtcgt cggccccacc gtcatctgcg ccatcaagca cggcgggctc gccatgacga
     3961 ctgccgcagc aacaggggct gggggtctcg tccgcgcaat caaacccaga cgcccgcgca
     4021 cccgacgttc ggttgaactc gcgatgttgg tgttcgcggt catcatcgcc ctcgccgcgt
     4081 acgccgacgt cggcctgacc cgctcacaac gggtgccgcc cgacatcttg acgtacggac
     4141 tcggccttgg cgcgctcgtc cttcttgcgc acattgcggt acgccgattc gcgccttacg
     4201 ccgacccgtt gctactgccg tgtgccgcac tgctcaacgg cctcggactc gtgttgattc
     4261 accggctcga tctggtagcg cccaaggccg cccggtcaga cgcaacgctg caactcgcgt
     4321 ggagtgcgat cggtgttgtt ggcttcatcg cggtgttagt gatcgtgcga gaccaccgac
     4381 ggctccagcg gttcacctat acctcgatgc tgctcggtct tgtgctgctc gcgattccgt
     4441 cgatcctgcc ggcccggttc agtgaggtca acggggcgcg caactggatc aggttcgccg
     4501 gtttttctat ccagccaggt gaattcgcga aggtgctgct cgtcatcttc gtggccggct
     4561 acctcgtcgc gaaacgtgac gcgctcgcgt tggccggcca ccggttcgcc ggcatcgatc
     4621 tgccgcgcgg tcgcgacctc ggcccggtcg tactcgcgtg gttggtcagt gtcggcctcc
     4681 tcgtgcgcgg ccgtgacctc ggcatgtcga tgttgttctt cggcttcttc atcttgatgc
     4741 tctacatcgc gaccgagcgg ctctcgtggc ttctcatcgg taccctactt ttcatcggtg
     4801 gcacgtatgt cgcctaccac ctcttcgcgc acgtacgcga gaggttcgag atctggttgc
     4861 atccgttcgc gcatgcgcaa ggctctggct accaactcgt gcagggcctg tacggctttg
     4921 cgaccggcgg actcatgggc accggtctag gcaacggacg ccctgatctc gtgccgttcg
     4981 cgaacagcga cttcatcatg gcgaccatcg gtgaggaact cggcctcacc gggctgatgg
     5041 cgatcctgct cgtgtacgtg gtgatcgtgt cgcggggtct tcgcgcagcg ctgtctgtgc
     5101 gcgactcgtt cgggaaattg ctggctgccg gactgtcggt ctgcctcgcc gtccaggtct
     5161 tcgtcgtcgt cggtggtgtg atgcggctga tcccgctcac cggtatcacc acgccgttcc
     5221 tctcgtacgg cggctcgtct ctggtgtcga actggatact gcttgccgtt ctgctacgga
     5281 tcagtgatgc cggacgtcga ccggtggccg cgcctacgcc ccggcccgac gacgccatga
     5341 cgcaggtgat ttcgctgtga acaagccaat ccgtcgagtg gcaatcacct gcctgctcct
     5401 gttcgccatc ttgctcgtga acgacaacgt ggttcagttc gccgacgcca agacgctgcg
     5461 cgacaagccc ggcaacactc ggttgctgtt ccagcagtac gaccgcaagc gcggcgacat
     5521 cgtgacgtcg accggggtca ccatcgccag ctcggtgccg acgaccgacg cgctgaaata
     5581 cttgcggacg tatccgaatg gcccgctctt cgcgcccgtg actggttact actcgatcgt
     5641 ctatggcgca tcacagatcg agagctcgga gaactcgatt cttgccggca gcgacgatcg
     5701 cttgttcggc gtgcagcagt tgtcgaacct catcactggc cgcacgcccg agggcggcag
     5761 cgtggttttg acgatcgatc cggtgttgca acaggtggcg tacacggcac tcgccgggcg
     5821 caagggcgcg gtcgtcgcac tcgacccgac taccggcgcg atcctcgcgc tggtcagctc
     5881 gccgtcgtac gacccgaaca cgctgtcgtc gcacgatcag acgacgatca cggcctactg
     5941 gaagaaactg attgctgaca ccgcaaaccc ggggctcgat cgcgcgctga accagaccta
     6001 tccaccgggc tcgacgttca agctgatcac cgcagccgct gcactctcga ccggcagcta
     6061 cacgccgtcg acggtgatcc ccgcgcccgc aacactaccg ctcccgcagt cgaccagcgt
     6121 cctgcacaac ttccaaggcg agagctgcgg cgggcaggcc acgctgatcc aggcgctgac
     6181 gatctcgtgc aacaccgcct tcggcggtct tggtttagca cttggtcaag acgcactgcg
     6241 cacccaggcc gacgccttcg ggttcgactc cccgtacacg gtgccgatga agtcggcaac
     6301 cagcgtgttc cctgacgact tgtctgacgc gaacacgatc cagtcggcga tcgggcagtt
     6361 cgacgttcga gcgacgccgc tgcaaatggc gatggtcgcc gcagcgatcg ctaacggcgg
     6421 tgtcgagatg aagccgtacc tggtcgacca actggtcgga cccgaccttc gcatcgtcga
     6481 ccagaccaag ccggcgccac tgcgtcgcgc ggtttcagcg caggtcgccg ccgagctcac
     6541 cctgatgatg gagagcgtgg tgcagcacgg cacgggcacc tccgcacaaa ttcccggtgt
     6601 cgcggtcgcc ggcaagaccg gcaccgcgca gaacgcgatc ggagccgcgc cacacgcgtg
     6661 gttcgtcgcg tttgcacccg ccgtgcagcc gaaaatcgcc attgccgttc tcgtcgagaa
     6721 cggcggaggc aacagcgacg cgacgggcgg cgcgatcgcc gcgcccatcg ccaaggcagt
     6781 catccaggct gaactgaaga agaaatgatg aggccggacg tgacgcgcag ttatcgatac
     6841 gcagggttca atcgagtagc gcctcaacag ggttcgacgg agcctaaggc gctcaacatc
     6901 aagagagggt tctcacatga ctgagccacg gcggctcggc gatcgctacg agctgggcga
     6961 agtgctcggc tacggcggta tggccgaagt gctccgaggt cgcgacatcc ggctcggccg
     7021 tgatgtcgca gtcaagaccc tccgcgtcga cctcgcgcga gagccgtcgt tccaggcccg
     7081 gttccggcgt gaagcgcagt cggccgcctc gctgaaccac ccggcgatcg tcgcggtcta
     7141 cgacaccggc gagagcttgc tcgacggcgt cccggtgcct tacatcgtca tggaatacgt
     7201 cgatggccga acgctgcgcg acgtgttgaa ggccgagcgg cacatcatgc cgacacgggc
     7261 gctcgagatc accgcgcaaa tcttgaacgc actcgactac agccaccgca acggcatcat
     7321 ccaccgcgac atcaagcccg cgaacgtcat gctcaccagg tcgggtgagg tgaaggtcat
     7381 ggacttcggc atcgcccgct cggtggccca agccagcgcc gctgtgacac agaccgcagc
     7441 cgtcatcggc actgcgcaat acctgtctcc tgagcaggcc cgcggcgagc aggtcgaccc
     7501 gcgtagcgac gtctactcga ccggctgcct gctctacgaa ctgctcaccg gcacgccgcc
     7561 tttcactggt gactcggctg ttgcggtcgc ctaccagcac gtgcgcgaag acccgattcc
     7621 gccatcgcga gtcaacccag atgtcagccc ggccatcgat gccatcgtgt tgaaggcgat
     7681 ggcgaagaat ccggccaacc gctatcaaag cgcggctgag atgcgcgaag acatcgagcg
     7741 tgcgctgagc ggcgttccgg tgcaggcgac cccgttgttg cacgaccaga cggtgagcat
     7801 cgccccgatc gtcgaagacg agtacgtgcg gcggcagccg cgcggtcggc gatcactgac
     7861 ctacatcatg gtgatcctga tcgcgatcgg gatgttcatc ctgatcggcg tcatagcccg
     7921 ccacctgctc accacgaaac atcaagcggt gacggtgccc gtgccgaacg tgtcgggctt
     7981 ctcccaatcc gacgcgatcg cgagcctcaa aagtgcgggg ctcaacccag tggtacttcc
     8041 gggcacgagc gcgacggtcg ccaagggcat cgtcataagc gagaacccgc cacagggcac
     8101 tccgttgccc ctgaagacca acgtcacgat ctacgtctcg accggaccac aagcggtgca
     8161 agtccccgac ctgacgaacc agtcgcttac cgacgccaag tcagagttac tgcgcaacaa
     8221 gctgattctc gggacgacga caaccaagga ctcgccgcag agcgcgaaca ccgtgctcag
     8281 tcagacaccc gcggccgggg cgaacgtcac ctcaggatct gccgtgaacg tcgtggtggc
     8341 cagcggcaac agcaccgtgc ccgacgtgcg caacctgccc gtcgccgaag cggagtcggc
     8401 cttgacgtcc ttcggattca acttccagga gatcgaccaa ccgagcagca ccgccgctcc
     8461 ggggattgtc atcaatcagt cgccgccgca cgactcgcca gcaaaaaccg ggaccatcgt
     8521 caagctgttc gtgtcggtcg caccgccacc gtcgtcgtcc ccgccagcga actcaccacc
     8581 gccgaccgcc acaccaacga acacagctac gacgtcgccg tcagcgtagc tacagcggcg
     8641 aactcagcaa gccggcgcga gctggtgcgt cggcatcgcc gcaccgcacg agccagttgg
     8701 cgagcatctg gtgaccgccc tgggtcagca ccgattcggg gtggaactgc acaccctcga
     8761 cgtcgacatc gcgatgccac agcgccatca cgacaccaga gtcagtgcgc gccgtcacct
     8821 caagacacgt cggcagggtc gactcgacga cagcaagtga gtggtagcgc gtggcggtga
     8881 acgggtcggg cagaccggcc aggacgcctg cgccgttgtg atgcaccgca cttgtcttgc
     8941 cgtgcaggag ttcgggtgcg cggttcacca cggcgccgta ggcgacagcg atcgcctgat
     9001 gccccaagca caccccgaag atcggaaccc gcccggcgac ctcgcggatc aacggaacgc
     9061 agacacccgc gtcctcgggc gtaccaggac cagggctcag cagcacaccg tcaaaatcga
     9121 gtgcatcgga cggcgagagc tggtcgttgc gcagcacgac acaggtcgcg tcgagctggc
     9181 ccagatactg gacgaggttg aacacgaagc tgtcgtagtt gtcgacgaca agaatgcgca
     9241 cgccgcgagt ctcccagatc gcagcggtgg tcgtcgtgcg actatcccgc gggcagcacc
     9301 gagggtcctc caggcaaggc cgacggcggc ggtagtgctg gcggcgacga tgacggaacg
     9361 gtctcctcga ccgacggcga gttcaccgtc acgtcgttcc atggcagccg tggctcggcc
     9421 caggggaaga tcacgaagaa cagcagcgca agcacgccga ccagcagcag cgcgcacccg
     9481 ccgaccttgg cgccgaatcc acctggcagc cgtctccaga tccatccgta catgcctagg
     9541 cccccttcag tgcgggcggc aggcctgcgg ccttcggcag cgccgattcg agtagaccgg
     9601 tgacaacgag ccgatgcgag gccgagtact tcggcgtgca ggtcgtcagc gtgatcagcg
     9661 cctgggtcgg cgtcgcgcgg ggttggccgg gcacggggag gatcacccca acgtcggtcg
     9721 gcagcacggt ctggatcgtc gtgacgcggt aggtgtagta ggtatcgcga gtttcgatga
     9781 cgatcgcatc gccttgggcc agattgccga gaccgttgaa cggcgcaagg taggtcgtgc
     9841 ggtgccctga gatcacgaag ttcccgaggc cgcctgggtc agcggtgccc gggtagtgac
     9901 cgggaccctt cttcaggtcg tcggtcgaga cgccttgcac gattgcgtag ccgtagttgg
     9961 caccaagccg tgggatccgg atgatgccga tggcgttggc aatcagcgtg accggcgcaa
    10021 cggtaggagc tgatgcggtt ggcacgctgc tcggactcgg gcccgatgcg gtagcgccgg
    10081 gtgatgcgga tgcgaccggc gcaggtgacg cccagctcgc cgccaagtcg cgtttgatct
    10141 gcgcctgcgc gtgttcggtg tacaggccgg taaaccacaa ctcgtacggg atgaacagaa
    10201 acacgatgac gccgagcgtg atcagcagct caccaatgag ccggatcacc tggttgagcg
    10261 gggaggtcct ggtcacgagg gcgaactccc gcgttgtcgt cacgaggtga ccgcccgcgc
    10321 gtatgccagc gtgagcggac cggtgaacgc cggcatcgtg acgtctccct cgagagcgag
    10381 cttgtagccg agctggtagg cgtcgacgta ctgcttgtag atgatcacat cgggtgcatg
    10441 gtcaagtgcc tgctgcaact tctgcggatc gccaattgct tcgacggtgt agggcggcga
    10501 atacacgaca ccgtgcagca acaaggtgtt gcctacacaa cgtacggcgc tcgtggaaat
    10561 cactcgttgg ccctgcaggg tcatcgcttc cgcgccaccc gcccacagcg cgttcaccac
    10621 ggcctgcacg tcttgctgat ggacgacgag atagtcaggc gggacggcgg tgcgttgctt
    10681 cccgggcagc gctggggcgt cgttcagcgt gacgctcacg gcgggaccgg tgaccggctc
    10741 gagaccggcc gcgttggcga aggcatcggc ctcgtcgcga gcttgggcca cccgactgtc
    10801 ggtcttagcc gcttgcgatg tttcggcctg aagtttgcgt tggagagctg caagctgggc
    10861 gccctgcgtg atgacggtgc gttgttggcg attgaccagg ctgttgacgt catcacggcc
    10921 ggtcacccgc agatcgaacc cacgtgacaa cgttgcggag gccgcgaaca tcagcccggc
    10981 gagcaaggcc gcgataggga cgccgtagcg ccaggcacgg gccgcgcgag tcgaagctgc
    11041 caccgccgcg cgcaccgccc ttcctcgact gcagttcctg accctcggcg gctacgctaa
    11101 ccgagtcgcc acagtgtcag acgcctctga agggttcgtc gtgccgaagt cgcgcgtccg
    11161 caagaccgag gtcttcactc cgccaccgcg gcggtccgcc aacaagcggg tcagcccgcc
    11221 gtggctcgcg ccgaccatgg tcggcttcat gctggtcggg gtgctgtggt tggttctcgg
    11281 ctacgtcacc aactacgcgt tccccggcat gtcgacgctg ggcagccagg gcggcaacct
    11341 gatcgagggc ttcgcgcttc tcgtcgtagg attgggcctc gcgacgcagt ggcgctagga
    11401 gtggcgagcg ctcgcgagcg cggtcagctg cccggttaac cctcaattca ccagctagtg
    11461 gtgaatgaca cacctgtaat tcatccacag cggttgtcca caatggggac aacgaccacc
    11521 tctggcgcgc cgaaccctgt ttctcgggtt ctcgagcgcc gctcagccgg tgaggttgtg
    11581 cgtgcgcagg acgaccgcga ccaccatcac ggcgatcagg gcgatcgaac ttccgatctg
    11641 gatcgccgtc cggctaggcc gtggcgcata ggcccaggct gctgcgagag cagcgcccgc
    11701 gaccagtccc ccgatgtggg cctgccagct gatacccgcg agggagaacg tcagcaccag
    11761 gttcaagccg atcgtcaccg cgatcggctg aatgttcaat cggagcttgc ggccaacgac
    11821 cagcaacgcc ccgaacagtc cgaagatcgc ccctgaggcg ccgagttccg gaacgttcgg
    11881 cgccgtcaag acgtaggcga gcactgagcc ggccaagcct gtcagcaggt acatcgccgc
    11941 gaacctcacg cgcccgagca gcgcctcgag cggcggcccg acgatgtaga gcgcgtacat
    12001 gttgaagagg atgtgcaaga tgaacaaccc gccggacgac ggtgggtcgt gcaggaacat
    12061 gctcgtaagt agccggtagt actcaccgtg cgcgaccccc gcaaccacgt gcaccttgcc
    12121 attggctgtg aagcccgtcg cgccgaactg ccagaaccgc tgcacgaatg cggtcgacgc
    12181 cacctcgccg aggtacgcca gcacgttaag accgatcaga gcatacgtga ccgcgtgcgg
    12241 gttcgcgctc ggacgcccac cgagcggcgt ccggccttgt cgcactgtct tttgtccgtc
    12301 ccgaacacac tcaggacatt ggaatccgac cgatgccgtg accatgcagt cgggacagat
    12361 cggtcgtccg caacgaacgc agctgacgta ggtctcgcgg cctgggtggc ggtaacacgt
    12421 aggtgcggcg ctgctgcggg caggcccacc tgggtctgca gccatacgcc tctagaccgt
    12481 gcggcgttcg acggtgatgg agttgatcac gatgtcagtg cgtggcttgt cgccagcacc
    12541 tgcgggtgcg gtcgcgatcg catcgacaac ctcactgccc ttcgtgacct cgccgaaaat
    12601 cgtgtgcttg cggttgaggt gcggggtagg cgcgaccgtg atgaagaact gcgagccgtt
    12661 cgtgttcggc cctgcatttg ccatggccag caagtagggg cggttgaagg ccaattcggg
    12721 gtggaactcg tcggtgaact ggtaacccgg acccccgcgg ccgctgccca acgggtcacc
    12781 gccctggatc atgaacccct cgatcacccg gtggaatatt gtgccgtcgt acagcttggc
    12841 cgtggtcttc cctccgcttc gcgggtcctt ccactccttg ccgccttcag cgagttctac
    12901 gaagttggcc accgtcttcg gcgcgtgatt gggaaaaagc cgaacctcga tgtcgccttt
    12961 cgtggtgtgg aaagtcgcga aagtctcgtc ggccactggg taattcctcc cggtcggttc
    13021 actgatacgt catcttccca cgcgttcgga gcggttggcg gcgatggcgg ttgggaaggg
    13081 catactggac tacaggcaaa ctgaacacga ggccgcttcc acggaggttc gcatgttcac
    13141 aaggaccgac aagatgacca gcaagctcaa tgacaccgtc accgcgcgag tcgccgcggc
    13201 gcgccagctg ctcgacgcca aggtcatccc ttacgccgtt ccggctggcc gcaaggctgc
    13261 caagcaggtt cgccaggtcg tcggcgagcg catcgttccc gtcgtcgaca acgcccgggt
    13321 tgcttctgcg ccggcccgcg cggaggcgtt ccgccggggg cggcttgcag cagccgcgtt
    13381 gcgcgggggt ggcacgatga tcgcgaagaa gcgccgccgc tggccggtgg ctctcgtctg
    13441 cctcgggctc ggcggcgcgc taggcgctgg cgcagcgtgg ctggcgcagg ccggcaagcc
    13501 cgtccagctg acgccctacc cgctcgacga cgacactgat gcgggccacg atcacgagca
    13561 ccactcgctc aacggatcgc accccctcga cctgaccgac gagtcgcacg cgcaccactg
    13621 atacacgcgc accactgaga tctgagccga gatttgggcc gagcgcgtca gccgggttcg
    13681 tgggctgacg cgctcgctgc atctttcgtc gcctgcaggc tgtcgaccgt cgcctgaagg
    13741 ctcaggtgcc aggcctgcca taggtcggca aacccgcgag tgcgcgggtc gccaccccgg
    13801 atttcgtcgt tggttccgtc gtccgtcacg ccgggcgcct cgagcatcgc gatcacctgg
    13861 agcgactcga gcgcactgcg ggcgagcgat accaaggctt cgtccgtcgt ttccaaagtc
    13921 aggaccgcgc ctcgtagatg cacggccagc tgacggcgtc cggcctcttc cggcagttca
    13981 tcggtggtcc cgaccagatc gacgctgcgc aatgccgtac gggcctgctc gagatcgacg
    14041 ccgagggtgc cgagcgcttt cgcggccgcg ctttgtggat cgttcagcgt tgccagcagc
    14101 aagtggtgcg atccgaccgc ggcctcaccg gcgagccgct gagcttcgtc gagcgtggcg
    14161 tcggtcgcgc cggtcgtgcg gatctgaatc gcagccgccg cttcgacttc ggcggagttc
    14221 cgcgatcgct gccagaacaa tcgacgcctc gactccgagg gccctgccgg cggcacgagg
    14281 tcgaggacct tcaaccgcac cgcgagcaga tcgccggcgg actcggtgag cacgcgagcg
    14341 ccgaggccgt cgccttcgcg gataaggccg agcagcaggt gctcggtgcc gatgtggtcc
    14401 gctcgcaggt gcaccgcctc gcgcaagctc agctcgagca ccttcttcgc tcgcggggtg
    14461 aaaggaatgt gccctaacgg cttccgcttg cccttaccca ccgccgcttc gacgctgccg
    14521 cgcgcaccgt cgagcgtcat gccggacgcg tcgagcgcct gcttcgcgac gccttcgggt
    14581 tgagccagca aaccgagcag caggtgttcg gtgccgatgt agttgtggct gagccgtcgc
    14641 gactcttcct gggcgaggac gacgacctgg cgagcctgtg cggagaatcg ttcaaacatg
    14701 tggctagcgt gcgccgcgct ctcaacccca gtcaagacca tgatattaat agggctgcca
    14761 gcgtagagag gtgagacctg tggagccgat cgattggaac gaccgcctcg acgcctggga
    14821 cgccgcgctt gagctgtcgg ctcagctcga tggatctgag tgggtcacgc tcgacgtcgc
    14881 tgcccgtcaa accggggcgt ctcgcgcggc ccttcgcacg tggtaccgca acgaggagat
    14941 cccgtcgcgg ctgaccgaag ggccgcacgg tccgacccgg ttggttccgc tgactgtcgt
    15001 cgcagcgcgt gccgaggcgt cgccccggct gaggtactcg gcgcaacgac ggctcgacgc
    15061 tgaagcgcag gtcgcgctgc ttcgccatcg cgtcgacgtt ctcgaaggtc gcctgtcggc
    15121 gctcgaatcg cgactgacgg cagcacaata aggcccgagc atgttggcct cagaggagcc
    15181 ggctgatgtc gttgaggttg tcgattgctg cgggcctgat ggtggtagcc gccaccgctt
    15241 gctcgagcac aatccaccga ccgcccgcga tcagcgtgtc gagcttcgcg ccggttccga
    15301 tcggctcggg atcggcgttg gaccagagtg gtgacaccgg cactcaggca ggtcaactcg
    15361 cgagccaact cgggtgcacc gacttctcgg cggatgtgca gctcgaccgc tacgcacaag
    15421 acgaagcgac ctgcctgatc aacggcgaca acgaggtgac gatctttgac ttcagcgggc
    15481 aggctgacga acaagcctgg ttcgccctcg gccccgaatc agctgacggc agcggcactg
    15541 tcgtgctcgg tggaggttgg gccgtggagc catccaatgc gggccaggcg gcgttcatcc
    15601 agcagacgct cggcggcacg atttcgcagc agcagggtct gggcggctcc agctaggcgc
    15661 ggcctggaca cgcgactcac gacattcgtg gagacgaggg gaatcgaacc cctgaccccc
    15721 gccttgcaaa ggcggtgctc taccaattga gctacgtccc ctgggaccac gcggtcgctc
    15781 gaaacggtga ccgttaggcc accggctcgg gctcgaccgc gctcgtcgcc tcgatccaga
    15841 gatcttcttc ggcttgctcc gcctgaactc gacggtaaat caccaggccg cccgcgagga
    15901 cgaccgccaa caacaggatc ttcttcatcg tttgccacct cgactcgacg acgggggtgg
    15961 gcctgggagg acttgaacct ccggcctcat ccttatcagg gatgcgctct aaccaactga
    16021 gctacaggcc ccggcttcgt gcctcgccga gggtatccca cggcctcggg tggggccatt
    16081 ccgagcgctt cacggatcgg tgagcgtgac ctcgactccg ccgaccaggt cggagatcag
    16141 gttgtagatc gcggcaccca gcgtcgacaa agccgtgagc aagacgacgt tgatcgcgcc
    16201 gatgacggcc gccactccca cgacccgccc ggcgctgacc ggattgtgca gcgtgctgct
    16261 cgtcgtcgtc ggcgagacct cgttcacgag agtgttgatc tgggtccaga cacccatccg
    16321 cccgagcacc gcgtagatca ccgccacagc gatgacgatg accgcgagca tcacgatcga
    16381 tacgacgaag ctgatcttca gcacagtcca cgggtcgatg tgcttgatga ccaaccgggc
    16441 ccgccgtggg ccgcgacgtg cgacagcaac ttgcactgcc tttgaccgga ccggggccgc
    16501 caatccgccg tcaccaccgc tgccgaccag gcccccactg tctgccggct tcgttgccat
    16561 ggatccagcg gccgccgcgc ccccccagcc ggttggcgca ctggccgcag gcacgggagc
    16621 cgaaagaacg gtcgcagggc cgtgttcgtc agtgtctgca ggcctgtcgg ggaccgactc
    16681 tacgagagct gcgggcggct cggccgtagc gacaggatcg ggcctggcgt aaggactgcg
    16741 cgccgggcgc ccgcccaggt ctgaatcgct cacgagtctc cctggtcgtc atcgaaggcc
    16801 cctagaccat ctgggccctg cgagtcgacc atgtcagtgc cggtcgaggc tggcgccggc
    16861 ggcggtgctg tcatcttccc gtctacccca ttgtgcccac cggactcctc tgccgcgccg
    16921 gaagcttcgg tgtcgagttc ggtgtcgagg tcagcatcga gctcgccgtc gagctcaccc
    16981 gcatcggcaa cttccgaggc cgcctcagcg ttccgcgtga taccgacaac cgcatcaccg
    17041 tcgccgaggt tgatcaatcg cacgcccatc gtggcgcgcg atgtcggtcg gacctcatcg
    17101 gaccgggtgc gaatcacacc gccacccgac gtgatcgcga agatctcatc gccgggatcg
    17161 acaatgagtg cgcccacaag ccccccgcga tcatcggtca gcttggcggt gagcacaccc
    17221 tgcccaccgc ggccctgaac ggggtactcc tcgatcggcg tccgcttcgc aaagccgcca
    17281 tcggtcgccg tgaagacaaa cgagtcaccc cgcgccacca tcatcgccag caactggtca
    17341 cccggccgga atcgcatgcc gatcacgcca ctcgtcgcac gccccatggg tcgcagtgct
    17401 tcgtcgttgg catggaaccg gatcgattga cccttgcggc taatgagcag gaggtcgtcg
    17461 gaggccgaca cgagccgggc cgcaatcacc gcgtcgtcgt cgcgcaggtt gatggcgatc
    17521 acgccaccgg accggttcga gtcgaactca cgcagcgcag tcttcttcac caaaccctcg
    17581 cgagtggcca gcacgagata gggagccacc tcgtagtcgc gaagcgcgag cacctgggcg
    17641 atcgtttcgt ccggctggaa ggcgagcaag ttcgcgacgt gctgcccccg cgcactacgt
    17701 gcggcctctg gcagttcata cgccttcgcg cgatacacgc ggcccttatc ggtgaagaac
    17761 agcagccagt gatgcgtggt cgtcacgaag aagtgctcga ccacgtcgtc ttggcgaagc
    17821 tgcgcgccga tgacgccttt gccaccacgg cgctgggcgc ggtaaaggtc ggtcttggtg
    17881 cgcttcgcat agccgccgtt gctgatggtg acgaccacat cttcttctgc gatgaggtct
    17941 tccatcgaga cttcgccctc ggcggcgatg atgtgcgacc ggcgctcgtc gccgtgcttc
    18001 tcgacgatct ccgccaactc atcgcgcacg atcgtgcgct gccgcggctc attgaccagg
    18061 atcgcgttca ggtcggcgat gtcggccgac aactcctcgt actcatcgat cagcttctgc
    18121 cgctcgagtg ctgccagccg ccgcaactgc atgtcaagga tcgcgttggc ttgagtctca
    18181 tcgatcgcca acagcgccat taggccctcg cgcgcgacct cgaccgtcgc actgttgcga
    18241 atcagtgcga tcacctcgtc gaggcgctcg agtgcggcaa gatacgcgcg cacgatatgc
    18301 gcgcgctctt ctttcttgcg caggcggtag cgagttcgcc ggacgatgac ttcgatctgg
    18361 tgcgcgatcc agtgccggat gaacccgtcg agtgccaacg tgcgaggcac gccgtcgacg
    18421 agtgccacca tgttgcagcc gaacgtgtct tgcagttggg tgtgcttgta gaggttgttc
    18481 aacacaacct tcgcaacggc gtcgcgcttg agcacgatga cgaggcgctg gccggtgcgt
    18541 gatgacgagt cgtcgcgaat gtcagcgata ccggcgagct ttccttcgtt cacgagctcg
    18601 gcaatgcgca atgcgaggtt gtcggggttc acctggtaag gcagctgggt gatgacgaga
    18661 agttgccggc cacgagtgtc ttcctcgacg tcgatgaccg cgcgcatcgt gaccgagccg
    18721 cggccagtgc gatacgcgtc ttcgatgcca cgccgcccga cgatgagacc acctgtcgga
    18781 aagtctgggc cggcgattcg ttcgagcaga gcctcgagca gctcttcgcg ggttgcatcg
    18841 gcatgctcga gggcccactg cgcgccggct gctacttcgc gcaagttgtg cgggggaatg
    18901 ttcgtcgcca taccaacagc gataccagcc gacccgttca cgagcaggtt gggaaaacgg
    18961 gatggaagca gcagcggctc catcgtcttg ccgtcgtagt tcgcagtgaa gtcgacggtc
    19021 tcttcgtcaa tgtcgtcgac catccgcatc gcaagttgcg ccatgcggca ttcggtgtaa
    19081 cgcatggccg ccggcgggtc gttgccgggc gagccgaagt tgccttgacc atcgacaagt
    19141 ggatagcgca acgaccacgg ctgtgcgagc cgcacgagcg cgtcatagat cgaggagtcg
    19201 ccatgcgggt ggtactgacc catgacgtcg ccgacaacgc gcgaacactt attgaacccg
    19261 cgatctggcc ggtaaccacc gtcgtacatc gcgtacagca cgcgacgatg aacaggcttc
    19321 aatccatcgc ggacgtcggg tagcgcacgc ccgacgatga cgctcatcgc atagtcgcga
    19381 taactgcgct gcagctcgac ctggagatcg accggttcga tcc
//
LOCUS       DASWVF010000209         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_209619, whole genome shotgun sequence.
ACCESSION   DASWVF010000209 DASWVF010000000
VERSION     DASWVF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209619"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /gene="ppk1"
                     /locus_tag="VGD55_03860"
     CDS             complement(<1..1748)
                     /gene="ppk1"
                     /locus_tag="VGD55_03860"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0869510.1"
                     /translation="MTQVSQSGSPVDAPDFSDELVEALDEAVTEHHAITSPDLPADRF
                     IDREASWLDFNMRVLELATNPDVPLLERVKFLAIFATNLDEFFMVRVAGLKRRIATGI
                     AVTAPSGLSARDQLELVSTYAHTLATDHARAFFDVIRPELAERGIHLLRWDDLDDADH
                     IKLRTFFNESVYPVLTPLAVDPAHPFPYISGLSLNLAVVVRDPTNGEERFARVKVPEI
                     LPRFLPVGEGRFVTIEEVIAAHLQVMFHGMEVLESHRFRVTRNEDLEVDEDDVDNLLQ
                     ALERELLRRRFGPPVRLEVEETTSPRVLDLLVRELGVNAAEVYRLPAPLDLAGLWSIA
                     ELQRPELKYEQFVPATHPKLVNPDGDGVRDIFTALRTGDILVHHPYDSFATSVQAFVE
                     QAATDPNVLAIKQTLYRTSGDSPIVDALVDAARAGKQVLVLVEIKARFDEEANIRWAR
                     KLEQAGCHVVYGLVGLKTHCKLCLVVRRESDGSLRRYAHLGTGNYNPKTARLYEDVGL
                     LTADPAIGGDVADLFNHLSGYTRPDPYKTLLVAPDTVRTGITLMIGREVDNAVAGRPS
                     GITMKLNSLVDEELID"
     gene            complement(1832..2506)
                     /locus_tag="VGD55_03865"
     CDS             complement(1832..2506)
                     /locus_tag="VGD55_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869511.1"
                     /translation="MVLQYCAVAFGLAALALLVRWLSQRTIRDANFPAISISSCLVLA
                     VVATFPILNRITLESRLSHAASQLIGVQVKVDCQTLTGSSLDVGRELGYVKWGPDGVP
                     ERKTLIKHTQCNDLNAYLNSNKLHPSAAQILAVHVLTHETMHMSGTTSESIAECHAMQ
                     HDAAMAEELGAPPAAAHSLAVIYFHTVYPDMPDGYTSSDCHQGGALDLHLPDAPWTGV
                     ASAATP"
     gene            complement(2560..>2803)
                     /locus_tag="VGD55_03870"
     CDS             complement(2560..>2803)
                     /locus_tag="VGD55_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0869512.1"
                     /translation="TMRAFAAAGISLPHSAAGQQHAGRRVPLSQLQPGDLVFWGSPAY
                     HVGIYVGGGRIIDAPHTGTDVQIQALWGRPSSAVRL"
BASE COUNT          491 a          870 c          958 g          484 t
ORIGIN      
        1 tcgtcgatca gctcttcgtc gacgaggctg ttcagcttca tggtgatccc actcgggcga
       61 ccggcgacgg cgttgtcgac ctcacgccca atcatgagcg tgatcccggt gcgcaccgtg
      121 tctggtgcga cgagcagcgt cttatacggg tcgggtcgcg tgtaaccaga gaggtggttg
      181 aagagatcag ccacatcgcc gccgatggct ggatcggcgg tgagcaaccc gacgtcctcg
      241 tataagcgtg ccgtctttgg gttgtagttg cccgtgccga gatgtgcgta gcgacgaagc
      301 gatccatcgg actcgcgtcg cacgacgagg cacagtttgc aatgcgtctt cagaccgaca
      361 agcccgtaca cgacgtggca gcccgcttgc tcgagcttgc gtgcccacct gatgttcgcc
      421 tcttcgtcga agcgcgcctt gatctcgacg agcaccagca cctgcttgcc tgcgcgtgcg
      481 gcgtcgacga gggcatcgac gatcggggaa tcgccactgg tgcggtagag cgtctgcttg
      541 atggccagca catttgggtc agtggcggcc tgctcgacaa acgcctgcac gctcgttgcg
      601 aacgagtcgt acggatggtg cacgagaatg tcgccagtgc gtagagcagt aaaaatatcg
      661 cggacgccgt caccgtcggg gttgaccaac ttcggatgcg tggccggcac gaattgctcg
      721 tacttcaact cggggcgctg aagttcggcg atgctccaca ggcccgcgag atcaagcggc
      781 gcaggcaacc ggtacacctc tgcggcattc acgcccagct cacgtacgag caggtcgagc
      841 acccgcgggc tcgtcgtctc ttcgacttcg aggcgcaccg gcgggccgaa ccgtcgacgc
      901 agcaattcgc gctcgagagc ctggagcagg ttgtcgacgt cgtcttcgtc gacctcgaga
      961 tcttcgttgc gcgtcacgcg gaaccggtgg ctctccaaga cctccatgcc gtgaaacatc
     1021 acctggagat gcgcggcgat gacctcctcg atcgtgacga agcggccctc acccaccggc
     1081 aggaagcgcg gcaggatctc cgggaccttc acacgcgcga atcgctcttc accgttggtt
     1141 gggtcgcgca ccacgacggc caggttcagc gacaggccgg agatgtaggg gaacgggtgc
     1201 gcgggatcga cagcaagcgg cgtgagcacc gggtacacgc tctcgttgaa gaacgtgcgg
     1261 agcttgatgt ggtcggcgtc gtcgagatcg tcccagcgca agagatggat gccacgctcg
     1321 gcgagttcgg gtcgaatgac gtcgaagaag gcccgcgcgt gatcggtcgc caacgtgtgc
     1381 gcgtaggtcg agacgagctc gagctggtcg cgcgccgaga ggccgctcgg cgcggtgacc
     1441 gcgatgccgg tcgcaatccg tcgcttcagg ccagcgacgc ggaccatgaa gaactcgtcg
     1501 aggttggtgg cgaagatcgc gaggaacttc acccgctcga gcagcggcac gtcggggttg
     1561 gtcgcgagtt cgagcactcg catgttgaag tcgagccagc tcgcctcgcg atcgatgaac
     1621 cggtcggctg gcagatccgg gctggtgatc gcgtggtgct cggtcaccgc ttcgtcgagg
     1681 gcctcgacca gctcgtcgct gaagtcgggc gcatcgaccg gcgagccgga ctgcgaaacc
     1741 tgcgtcacgt cgacctcatc tgcgcgatcc gcgaatgcac ccccgggttg gaggtgcgga
     1801 cgtgtggggg cgtgatccgc cggggcccag cctacggcgt cgcagcgctc gctacacccg
     1861 tccaaggcgc gtctggcagg tgcaggtcga gcgcgccgcc ctggtggcaa tcggagctgg
     1921 tgtagccatc gggcatgtcc ggatacacgg tgtggaaata gatcacagcc agtgagtgcg
     1981 cggcggccgg cggcgccccg agttcttcgg ccatggcagc gtcgtgctgc atcgcgtggc
     2041 actcggcgat cgattcagac gtcgtccccg acatgtgcat cgtctcgtgg gtcagcacgt
     2101 ggaccgccag aatctgcgcg gcactggggt gcaacttgtt cgagttgaga taggcgttca
     2161 ggtcgttgca ctgggtgtgc ttgatcaggg tcttgcgctc cggcacaccg tcgggtcccc
     2221 atttgacgta cccaagctcg cgaccgacgt cgagcgacga gcccgtcagc gtctggcaat
     2281 ccaccttgac ctggacgccg atgagttgcg acgccgcgtg gctgagccgg ctctcgagcg
     2341 tgatccggtt caggatcggg aaggtggcga cgacggccag gaccaggcac gaactgatgc
     2401 tgatcgcggg gaaattcgcg tcacgaatcg tgcgctgcga gagccagcgc accagcaacg
     2461 cgagcgcggc gagcccgaag gcaacggcgc agtattgaag gaccacgcct gcgtatcggc
     2521 aaccaagcgg cgctcactga gccgcgctaa ccccgaacgt cagaggcgga cggcgctgct
     2581 gggacggccc caaagagcct ggatctggac gtcagtgccg gtgtgcggtg cgtcgatgat
     2641 gcggccgccg cccacgtaga ttccgacgtg gtaggcgggg ctgccccaaa acaccagatc
     2701 gcccggctga agctgcgaca ggggcactcg gcgtccggca tgttgttgcc ccgcggccga
     2761 atgcggtagc gagatccccg cggccgcgaa cgcgcgcatc gtc
//
LOCUS       DASWVF010000210         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_107678, whole genome shotgun sequence.
ACCESSION   DASWVF010000210 DASWVF010000000
VERSION     DASWVF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107678"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2424)
                     /locus_tag="VGD55_03875"
     CDS             complement(<1..2424)
                     /locus_tag="VGD55_03875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0869513.1"
                     /translation="MSFKQDRTTPPGPAPPGRSAAGTPVTSDTLKTSQVVAAKRPPPL
                     EPGVILDRRYAIEACVGEGGSGFVFRAFDRTLAQRIALKVLHPDLAADKSWIKRLKRE
                     VRVAREIQHPNVCRVFDLGHADGHWFVTMEYATDGSLRQVLDARSAMFRPLKKLPIAG
                     LAARFADAQAVCAGLAAIHSVGIVHRDITPGNVLRMNDGRLVITDFGLAISAKDVTTF
                     HGGTPKYMAPELLERKPADQRSDVWQLGYLLHEIIFQRHPDWTHIGDRVVLRSPAHEL
                     STPTERAIAELCIQCLSHNPDGRPKDAIQVAGRVAAAEHARPLPPLSRLYQAVRRFVG
                     RRVIRRGAVVLVAAACVWIGTRSLFHPSSPDRLLLRAAALWNSEEEDRARRPFVTVLS
                     SRPLAVDTFHRVSASIRDHLHNWTFAYGVARKRTGTGTGPDMQCLEDDLDSVAGVVTL
                     LQSDHAKETLVDGAPYAVAALRDPRRCLSSSRPTIVLPRPPPGTALRAQVDHLRRALM
                     FANPLMEGEHFAVAPAVIQPLVSAAKATGYRPLIAEALLAEAHAAGRAMTTDATYRAR
                     LEDALWEAERCGHDRIVAISAAELAFADRFSTDTAITSKWLRMADSVLDRIGGDSAIQ
                     SWLENNRGIAAHSRGRFADAIAAYQKALELKRAEVGPDHLEYAIRLLNLSEAFQGANR
                     LQDALHANDAAIRLMTSWLGPDHIDIGIAKNNRADILMALNRVDEAVHTYEAALDIFR
                     QKLSAQDPRLGFPLLGLGTALLQQGDHHRARLTLQQASAFDFTGDQFVVSDVKFALAR
                     ALVADGADHE"
     gene            complement(2901..3176)
                     /locus_tag="VGD55_03880"
     CDS             complement(2901..3176)
                     /locus_tag="VGD55_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869514.1"
                     /translation="MISSLFNSQGPVESDAVPRFVAGRLSNGHFGVFDVLLESYVVRD
                     VTLVFADRVAQRLNRPDTWEPTPHQLVADNVRQALTTPPPGSVSHIQ"
     gene            3293..3502
                     /locus_tag="VGD55_03885"
     CDS             3293..3502
                     /locus_tag="VGD55_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869515.1"
                     /translation="MPNRLHIDKGDAQDLVRLNVRVPRWLKAAIAEAMAVPGKGLNEW
                     VRDALRAALPSHLRRRSEARAARRA"
     gene            complement(3576..4244)
                     /locus_tag="VGD55_03890"
     CDS             complement(3576..4244)
                     /locus_tag="VGD55_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEY0869516.1"
                     /translation="MNDRDLTDRPEVPRASPTVPAADREPPDRTAAELLSGVLDVKVA
                     TAHRVLSTIGGVKALCAAPEAELVAAGIHAAKARRLRNAMALAQAAIGQRPIRGQRLT
                     GAAEVSAHMRARLADLPTEEFWAIAVDVRHRVILDELFARGSLSGVEIHPRDVFRPLI
                     RAGAAAVVFCHNHPSGDPTPSRVDIDLTARLRQVGDLCGIVVLDHVVVAADGYVSLAE
                     RGWR"
BASE COUNT          756 a         1309 c         1537 g          748 t
ORIGIN      
        1 ctcgtggtca gctccgtcgg cgacgagagc cctggcgagc gcgaacttga cgtcgctgac
       61 gacgaactga tctcccgtga aatcgaatgc agaggcctgc tgtagcgtca ggcgagcacg
      121 atgatgatcg ccctgctgaa gcaacgcggt gccgagacca agcaaaggga accccaggcg
      181 cgggtcttgg gcggaaagct tctgccgaaa gatgtcgaga gcggcttcgt aggtgtgaac
      241 agcctcgtcc actcggttga gcgccatcag aatatcggcg cggttgttct tcgcgattcc
      301 gatgtcgatg tggtcgggac cgagccagct cgtcatgagc ctgatcgcgg cgtcgtttgc
      361 gtgcaatgcg tcctgaagac gattggctcc ctggaacgct tccgacaggt ttaggagacg
      421 tatagcgtac tcgaggtggt caggtccgac ttcagcccgc ttcaactcca gcgccttctg
      481 gtaggcggcg atcgcgtctg cgaatcggcc ccgagaatgg gcggcgatgc cgcgattgtt
      541 ctcgagccag gattgaatgg cggagtctcc gccgatccgg tccaaaacgc tgtccgccat
      601 gcggagccac ttcgaagtga tcgccgtgtc cgttgagaaa cggtcggcaa acgccagttc
      661 ggcagcgctg atagcgacga ttcggtcgtg accacagcgc tcggcttccc aaagggcgtc
      721 ttcgaggcgc gcgcgatacg tagcatcggt tgtcatggcc cggccggcgg catgcgcctc
      781 agcgagcaga gcctccgcaa tgagcgggcg gtagccagtc gccttcgcgg ccgacacaag
      841 gggttggatc acggccggtg cgacggcgaa gtgctcgccc tccatcagcg ggttggcgaa
      901 catgagagcg cgacgaagat gatcgacctg agcgcggagc gcggtgccgg gtggtggccg
      961 cggaagcacg attgtaggcc gagaagacga caagcatcgt cgggggtccc ggagcgcggc
     1021 cacagcgtac ggagcgccgt cgacgagagt ttccttcgcg tggtcactct gaagaagggt
     1081 cacgacgccc gcgacgctgt cgagatcgtc ctccagacac tgcatgtcgg gacccgtgcc
     1141 ggtaccggtt cgcttgcgcg ctacgccata cgcgaacgtc cagttgtgga ggtggtcgcg
     1201 aatggatgcg ctgacgcggt ggaaggtgtc gaccgcaagc ggacgcgatg agagaaccgt
     1261 tacgaacggc cgccgcgctc gatcttcttc ttcggaattc caaagggcgg ctgcacgcaa
     1321 caggagcctg tcgggcgacg agggatgaaa gagagagcgc gttccgatcc agacgcacgc
     1381 tgctgctaca agcacaacgg cgccgcgtcg gatgacccgc cggcccacaa atcgccggac
     1441 cgcctgatac aagcggctga gcggcggcag cggcctcgcg tgctcagcgg cagcgacacg
     1501 acccgctacc tgaatggcgt ccttcgggcg cccatcaggg ttgtgggaca ggcattggat
     1561 acacagctcg gcgatggccc gctccgtcgg cgttgagagc tcgtgggccg gggatcgaag
     1621 gacgacacga tcgccgatgt gggtccagtc gggatgccgc tgaaagatga tctcgtgcag
     1681 caggtatccg agctgccaca cgtctgaccg ctggtcggcg ggcttccgtt cgagcagctc
     1741 aggggccatg tacttcggcg taccaccgtg gaacgtggtg acgtctttgg ccgagatcgc
     1801 caggccgaag tccgtgatga ccagccgccc gtcgttcata cggaggacat ttccgggtgt
     1861 gatgtcacgg tgaacgatcc caaccgagtg gatcgcggcg aggcccgcgc acactgcctg
     1921 cgcgtcagcg aagcgtgccg ccaaccccgc gattgggagc tttttgaggg ggcggaacat
     1981 ggcggaacgg gcgtcgagaa cttggcgtag gctcccgtcg gtcgcgtact ccattgttac
     2041 gaaccaatgc ccatcggcgt gacccaggtc gaacactcgg cagacgttcg gatgttggat
     2101 ttcgcgagcg acccgaactt cgcgcttgag gcgcttgatc cagctcttgt cggcggccag
     2161 gtcgggatgg agcaccttga gtgcgattcg ctgcgcgagg gtccggtcga aggcgcggaa
     2221 tacgaacccg gacccacctt caccgacgca agcctcaatg gcgtaccgac ggtccaggat
     2281 gacgcccggt tccaagggcg gcggcctttt cgccgcgacg acctggctcg tcttgagggt
     2341 gtcgctcgta acaggcgtgc cggcggccga cctcccgggc ggcgcggggc cgggcggagt
     2401 agtccggtct tgcttgaacg acacgatccc ttattcgctt atcgaggctt aggcgggagc
     2461 gcttgaggac cagctctcgg cgcctgtgga ggctgaatgc gcaggtgcga cttgcgacaa
     2521 ccgcctagac atgccgcggc gtgtgccgtt cgcgacgcga tcgagcgatc gtcgcctgat
     2581 ctcgtttttg cgggaaagct gtagtcttgg gccggctccc gcggccgtag gctgcgccgc
     2641 ggcactaccg gcttcccggc ttcgacggga tgaagctcag ccaacgggag gtcgaatcgc
     2701 tggtaagtcg tgggtttctg acggtcgcca cgatgatcac gggggagggc gagcatttca
     2761 cggtggcaat tggcgcccgc gcggagttcc ggcatttggt cgttgtctgc ggcgtaagcg
     2821 ggctccccgg tgagcgcagc ggtggcgcct tcagcccggc ccgtgatcag aagagcgcga
     2881 ggtagaccgc gcccctcgtc tcactgtatg tgcgaaaccg atcctggcgg aggcgttgtc
     2941 agcgcctgcc ggacgttgtc cgcgacgagc tggtgagggg ttggctccca cgtgtctggc
     3001 ctgttcagtc gctgagcgac ccggtcggcg aagaccaaag tgacgtccct gacgacgtag
     3061 gactcgagga ggacgtcgaa gaccccgaaa tgcccgttcg acagccgacc ggcgacgaac
     3121 ctgggaacag cgtcactttc gaccggaccc tgtgagttga aaagcgagct gatcatggac
     3181 acgcctccgt aaagacacct tcatggtgtc ttcacagcct acgcgaacgt tcagcgtcgt
     3241 caacatgaaa ggcgtcttga tgacgtcttc actgccagat agagtgagat gcgtgccgaa
     3301 ccggctgcac atcgacaagg gcgacgccca ggacctcgtc aggctgaacg ttcgcgtgcc
     3361 gcggtggctg aaggcggcca tagccgaggc gatggcggta cctggcaagg ggcttaatga
     3421 gtgggtgcgg gatgcgcttc gcgccgccct gccctcccat ctgcgccgcc ggtcagaggc
     3481 cagggccgcg cgccgagcat agggcgggcg gcagcgcgcc atggcgagcg cagcgcgccg
     3541 gcgcgctgcg ctctggtggc agttggcgct gcggctcagc gccagccgcg ctcagcgagg
     3601 ctgacgtatc cgtcggcggc gacaacgacg tggtccagga cgacgatgcc gcagagatca
     3661 ccgacctggc ggagccgtgc ggtgagatcg atgtcgacgc gagagggggt cggatcaccg
     3721 gagggatggt tgtggcagaa gacgactgct gctgccccgg ctcgaatcag cggccggaag
     3781 acgtcgcgag ggtgaatctc gacgccagac aggcttccgc gagcgaagag ctcgtccaga
     3841 atgactcggt ggcgaacgtc gacggcgatg gcccagaatt cttcggttgg cagatcggcg
     3901 agtcgcgcac gcatgtgagc cgagacttcg gcggcgcccg tgaggcgctg accgcgaatt
     3961 ggccgttggc cgattgccgc ctgcgcgagt gccatggcgt tgcggagccg tcgcgccttg
     4021 gcggcatgta tgccggccgc cacgagttcc gcttccggcg cggcgcagag ggctttgacg
     4081 cccccgatgg tcgaaaggac acgatgtgct gtcgcaacct tgacgtcgag tacgccggag
     4141 agaagctctg cggctgtccg gtcgggcggt tcccgatcgg ccgcagggac ggtggggctg
     4201 gctcgaggca cttcgggtcg gtctgtcagg tcgcgatcgt tcatgggctc ctccttgtgg
     4261 tcgggacgcc cgtggggagc gggccgagct ccccgtgcgc cggaggaccg ctttggtggg
     4321 gggaacccgc tccgcggggg gagccgccaa
//
LOCUS       DASWVF010000211         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_464408, whole genome shotgun sequence.
ACCESSION   DASWVF010000211 DASWVF010000000
VERSION     DASWVF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464408"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VGD55_03895"
     CDS             complement(<1..1092)
                     /locus_tag="VGD55_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0869517.1"
                     /translation="MTETGGPAVVAAIVLAAGEGSRMKSSTPKVLHELCGRTMLGHVI
                     AAVRGAAPAQVVVVTGQGRERVEAHLAHIDPDAIAAFQPSQEGTGHAVRIALAALDAA
                     AGPGLIEGTIIVVPGDAPVLAASSLRAIADRREETGAACVLLTANVPDADGYGRIVRD
                     DWGRITQVIEHVDATDAELDITEVNSSVYAFDAATLRLALAELTAHNAQGEQYLPDVV
                     RIMVKNADPVEAVVVHDWREIAGVNDRVQLAQARALLRDRIVTSLMRDGVTVIDPATT
                     WVDVGVVAERDCVVLPNTMLLGRTKIAMGARIGPNCSLTDTVVGAGAVVRDATCEGAH
                     IGPQATVGPYTYLRPGTRLARGAKAGGFVE"
     gene            complement(1122..1192)
                     /locus_tag="VGD55_03900"
     tRNA            complement(1122..1192)
                     /locus_tag="VGD55_03900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Gln,seq:ttg)
     gene            complement(1237..2163)
                     /locus_tag="VGD55_03905"
     CDS             complement(1237..2163)
                     /locus_tag="VGD55_03905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0869518.1"
                     /translation="MTPDTADAIRAVVITYNTRDLVIPLLGDLLTELKPWPGSEVVVV
                     DNASADETVDAIAAAYPDIRLEASKKNLGFGAAVNRAAQGATTRWLLLVNPDARIDAG
                     SVAKLVDVASRNPGHGLYGGRFVDIVRQTAEDSVAVVPTMTNLIAFAFGTGAIARRLG
                     WRSAPRRAAAANEPTVVEALPGTFLLIEADAWRAVDGFDERYFMYSEDLDLSLRVGAT
                     GRRPMYVPAASLQHAGGASSSSGAKEVLKLTSLVTLLRAQWPAPRARAGEAMLLTGVA
                     LRRIARLRARTPQGRWETAWRERARWRAGWAK"
     gene            complement(2153..>2737)
                     /locus_tag="VGD55_03910"
     CDS             complement(2153..>2737)
                     /locus_tag="VGD55_03910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0869519.1"
                     /translation="HDAAGRVVSQRKRAVPDAVARLASPATWAHAVRLVNFYGYAHVQ
                     QRRLLTAGPGLRMSPSVSMRNGQRITLGREVHVGERSCLWAGDSSGRITLGDNALLGP
                     EVFITASNYGVNAGTPVMYQDKVEADVVIGADVWLGVRVTVLPGVVIGDGAVVGAGSV
                     VTKSLPANCIAVGVPAKVVGWRPGSTPESSAGDA"
BASE COUNT          459 a          973 c          879 g          426 t
ORIGIN      
        1 ctcgacgaag ccgccggcct tcgcaccgcg ggcaagccgg gtgcctgggc gcaggtaggt
       61 gtagggacca acggtcgcct gcggcccgat gtgcgcgcct tcgcaagtcg catcgcgaac
      121 taccgccccc gccccgacca ccgtgtcggt gagggagcag ttcggtccga tacgcgcgcc
      181 catcgcgatt ttggtcctcc cgagcaacat cgtgttaggc aacaccacgc agtcgcgttc
      241 ggcgacgacg ccgacatcga cccatgtcgt cgccggatcg atgacggtaa ctccgtcgcg
      301 catcaagctg gtgacgatac ggtcgcgcaa cagggccctg gcctgtgcca gttgcacccg
      361 gtcgttgaca cccgcgatct cacgccagtc gtgcaccacc acggcctcga ccgggtcggc
      421 gttcttcacc atgatgcgga cgacgtcggg caggtactgc tcaccctggg cgttgtgagc
      481 ggtcagttct gccaacgcca gacgcaacgt ggcagcgtcg aacgcgtaca cgcttgagtt
      541 gacttcggtg atgtcgagtt cggcgtcggt tgcgtcgacg tgctcgatga cctgcgtgat
      601 acgaccccag tcgtcgcgca cgatccggcc gtatccgtct gcatcaggca cgttcgccgt
      661 gagcagcacg caagctgcac ccgtctcctc gcggcggtcc gcgatagccc gcagcgatga
      721 agcggcgagc accggcgcgt ctccgggcac gacgatgatg gtgccctcga tcaacccagg
      781 cccggctgcg gcatcaaggg ctgcgagagc gatgcggacg gcgtgcccgg tgccctcttg
      841 cgacggctgg aaagcggcga tcgcgtcggg gtcgatgtgc gcgaggtgcg cctcgacccg
      901 ctcgcgacct tgtccggtga cgacgacaac ctgcgcaggc gctgcgccgc gaaccgcggc
      961 aatgacgtgg ccgagcatcg tgcgcccgca cagttcgtgg agcaccttcg gcgtactcga
     1021 cttcatcctc gacccctcgc ccgcagcaag gacgatcgcc gcaacgacag ccggcccacc
     1081 tgtctcggtc acgcgcagct acctcccaga tgatttctga gctcccgggg gaggagtcga
     1141 acccccattg atgggaccaa aacccactgt cctgccatta gacgacccgg gatcggtgtc
     1201 cgcgcgccaa gactagccag ccacgcgccg tccggcctat ttcgcccaac cggcacgcca
     1261 gcgcgcgcgc tcgcgccaag ccgtctccca gcggccctgc ggggttcggg cccgcagccg
     1321 cgcgatccga cgcagcgcaa cgccagtcag cagcatcgcc tcaccggcac gtgctcgagg
     1381 cgctggccac tgcgcgcgca gcagcgtcac gagcgaggtg agcttcagga cttccttcgc
     1441 gccgctcgac gacgacgcac cgccggcatg ttgcagcgag gcggcgggga cgtacatcgg
     1501 acgccgaccc gtggcgccga cccgaaggct caggtcgagg tcctcgctgt acatgaagta
     1561 ccgctcgtcg aacccatcga ctgctcgcca tgcgtcggcc tcgatcagca ggaacgtgcc
     1621 gggcaacgcc tcgacgacgg tcggctcgtt cgcggcagcc gcccgtcgcg gtgcgctgcg
     1681 ccaacccagc cgccgggcga tcgcgcccgt accgaaagcg aaagcgatca ggttggtcat
     1741 ggtcggcacc acggcgacgg aatcttcagc ggtctggcgg acgatgtcga cgaaccggcc
     1801 accgtagagg ccatgccccg ggttgcgcga cgcgacgtcg acgagcttgg ccaccgaccc
     1861 cgcgtcgatt cgggcgtccg gatttaccag caatagccag cgggtcgtgg ccccctgcgc
     1921 agcgcgattc acggctgcgc cgaacccgag gttcttcttc gacgcctcga gtcggatgtc
     1981 ggggtaggca gccgcgatcg cgtcgacggt ctcgtcggcc gacgcgttgt cgacaaccac
     2041 gacctcggac cccggccatg gcttcagctc cgtgagcaga tcgccgagca gcgggatgac
     2101 caggtcgcgc gtgttgtagg tgatgacgac agccctgatc gcgtctgcgg tgtcaggcgt
     2161 caccggcact actttccggg gtacttcctg gtcgccaacc gacgaccttg gccggcactc
     2221 ccaccgcaat gcagttggcg ggcagcgact tggtcacgac ggatcccgcg ccgaccaccg
     2281 cgccgtcgcc gatcacgaca ccgggcagta cggtgacgcg gacaccgagc cacacgtcgg
     2341 cgccgatgac gacgtcggcc tcgaccttgt cctggtacat caccggagtt ccggcgttga
     2401 cgccatagtt gctcgcggtg ataaagacct cagggccgag caatgcgttg tcgcccaacg
     2461 tgatgcgccc gctcgaatcg ccggcccaca ggcacgaccg ctcaccgacg tgcacctcac
     2521 gaccaagcgt gatgcgctga ccgtttcgca tcgacacaga tggtgacatc cgcaacccgg
     2581 ggccagcagt gagcagccgt cgctgttgca catgggcata gccatagaaa ttcactagcc
     2641 ggacggcgtg tgcccacgtc gcaggactcg ccagcctcgc aacggcatca gggacggctc
     2701 gcttgcgctg gctcacgacg cggcctgccg cgtcgtg
//
LOCUS       DASWVF010000212         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_362338, whole genome shotgun sequence.
ACCESSION   DASWVF010000212 DASWVF010000000
VERSION     DASWVF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362338"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..279
                     /locus_tag="VGD55_03915"
     CDS             64..279
                     /locus_tag="VGD55_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869520.1"
                     /translation="MTVTTLIYAVDNQQAKSPNGALGLLVFLILAVAVVFLLRSMTKH
                     LRKVERINFDEPPKTGDDASGTIPPPR"
     gene            complement(284..721)
                     /gene="arfB"
                     /locus_tag="VGD55_03920"
     CDS             complement(284..721)
                     /gene="arfB"
                     /locus_tag="VGD55_03920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY0869521.1"
                     /translation="MPDVVSDLVVTSRVTVPASELRWRFSRSSGPGGQGVNTTDSRVE
                     LSFDVATSPSIPPFLRERAVSRLGDRLIDGVLVVSASEHRSQLRNREAAAARLVSVLR
                     GALAPPPRTRRPTMPSRGAIERRLTDKKATAKRKADRRRPPEW"
     gene            837..>1995
                     /locus_tag="VGD55_03925"
     CDS             837..>1995
                     /locus_tag="VGD55_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY0869522.1"
                     /translation="MLTELTEDACYRALSSRDSRFDGWFFVGVLTTGIYCRPSCPAMT
                     PQRKNVRFLPTAAAAQEAGFRACKRCRPDAVPGSPEWNVRADLVGRAMRHIADGVVDR
                     EGVAGLAQRLGYSERHVHRQLVAEVGAGPIALARAQRSQTARVLLETTDVAIANVAFA
                     AGFASVRQFNDTIREVFALTPSQLRASRRPATRTPTPGALHLRLPFRRPFAPLHTFDF
                     LATRAIPGVETGTPMGYRRTLSLVHGPAVIALGDVSEVGAQSRPSDDQTWIPCRLELS
                     DLRDLAPAVARCRRLLDLDADMQAVSEVLGDDPLLSASISAEPGRRVAGHVDGFELAV
                     RAVLGQQVSVAGARTLAARLVAALGEPVTTATGELTHLFPTAQAVAAADPAA"
BASE COUNT          325 a          676 c          648 g          346 t
ORIGIN      
        1 gttcgtgaac tccacgattc ccgaagacga cttgttcgcc ggcatccgcg aagacgcgcg
       61 cacatgacgg tgacaacgtt gatctatgcg gtcgacaacc agcaggccaa gagccccaac
      121 ggtgcactcg gattgctcgt cttcttgatc ctcgcggtgg ccgtggtctt tttgctgcgc
      181 tcgatgacga agcacctgcg caaagtcgag cgcatcaact tcgacgagcc gccgaagaca
      241 ggcgacgacg catcgggcac gatcccgccg ccgcgttaac gcatcaccac tcggggggcc
      301 tgcgccggtc tgccttgcgc tttgccgtcg ccttcttgtc ggtaagtcgg cgttcgatcg
      361 cgccgcgcga tggcatggtc ggacgccggg tgcgcggggg cggtgccaat gcgccacgca
      421 gtaccgaaac caggcgcgct gcggccgcct cacggttgcg cagctgagag cggtgttcgg
      481 acgccgacac taccagcact ccgtcgatca gccggtcgcc aagacgtgac accgcgcgct
      541 ctcgcaggaa cgggggaatc gacgggctcg tcgccacatc gaacgacaac tcgaccctgc
      601 tgtcggtcgt gttgacgcct tgtccgccgg gtccgctcga tcgcgagaat cgccaccgca
      661 gctcggaggc aggcacggtt acccgggagg tgacgacgag atcggagacg acatcaggca
      721 cggcgacgat tatccggctc agccgagact agcgggattc ggacgttgtc gacaggctgc
      781 gcgcagcgtc cgaaatccgc cagcagattg tcagaccgag ggtgcatgct cgtctcatgt
      841 tgaccgagct gactgaagat gcctgctacc gagccctctc aagccgcgac tcgcggtttg
      901 acggctggtt cttcgtcggc gtgctgacca ccggcatcta ctgccggccc agctgcccag
      961 cgatgacgcc ccagcgcaag aatgtgcggt tcctgccaac ggctgctgct gcgcaggagg
     1021 ccggattccg cgcttgcaag aggtgccggc ccgacgccgt tcctggatcg cctgagtgga
     1081 acgtgcgagc agacctcgtc ggccgcgcga tgcggcacat cgccgacggg gtggtcgatc
     1141 gcgagggtgt ggccgggctg gcgcaacgac tcggttacag cgagcggcac gtgcatcgcc
     1201 aactcgtcgc tgaagtcggt gccggaccga tcgccctggc ccgggcgcaa cggtcgcaga
     1261 cagcgagggt gttgctcgag acgaccgacg tcgcgatcgc gaacgtggcc ttcgcagctg
     1321 ggttcgcgag tgtgcgtcag ttcaacgaca cgatccgcga ggtcttcgcg ctcacaccgt
     1381 cgcaactgcg cgccagccgg cggccggcga cccgtacgcc gacgcctggc gctcttcacc
     1441 tgcgcctccc attccgccgg ccgttcgcgc cactgcacac cttcgacttc cttgcgacac
     1501 gagcaatccc gggagtagag accggcaccc caatgggtta ccggcggacg ttgtcgctcg
     1561 tgcacggccc tgcggtcatc gccctgggcg atgtcagtga ggtaggcgcg cagagtcggc
     1621 cgtcggacga ccagacttgg attccttgcc ggctcgaact ttccgacctt cgcgatctcg
     1681 cgcctgccgt cgcgcgctgc cggcggctgc tcgaccttga cgccgatatg caagcagtgt
     1741 ctgaggtcct tggcgacgat ccgctgctga gtgcatcgat ttccgccgag cccggacgtc
     1801 gagtggctgg gcatgttgac ggattcgaac tcgctgtgcg cgccgtgctt ggccagcaag
     1861 tcagcgtggc cggcgcccgc acgctcgcag cccgacttgt cgccgctttg ggcgaaccgg
     1921 tcaccacagc aactggtgag ctcacgcatc tttttccaac cgcgcaagca gtcgcggcag
     1981 ccgaccctgc cgcct
//
LOCUS       DASWVF010000213         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_260780, whole genome shotgun sequence.
ACCESSION   DASWVF010000213 DASWVF010000000
VERSION     DASWVF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260780"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="VGD55_03930"
     CDS             complement(<1..1863)
                     /locus_tag="VGD55_03930"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0869523.1"
                     /translation="MTATDSAGSADAAGSAGAAGPSSSDLPSVYAPAAVEATLYERWV
                     ERGYFTADVASGKPPFTIVLPPPNVTGQLHMGHAPQHTLMDAVTRRRRMQGYEALWLP
                     GMDHAGIATQNVVERELGKEGVSRDDLGREAFVDRVWQWKAEYGGRILSQMRRLGDGV
                     DWSRERFTMDAGSSRAVGTIFKRLYNDGLIYRAERIINWCPRCLTALSDIEVDHSDDE
                     GELVSISYGPITVATTRVETMLGDTAVAVHPDDERYAHLVGTEIELPLTGRRIPVVAD
                     DHVDPEFGTGAVKVTPAHDPNDFEIGQRHGLPSITIMDERGVITAHGPFQGLDRFEAR
                     PAVTAALRELGLIVAEKRPYVHSVGHCSRCDTVIEPRLSLQWFVKVESLAKAAGDAVR
                     DGRVTIHPPEMARNYFDWVDNMHDWCISRQLWWGHRIPVWYGPAGETVVIGPDDDAPT
                     AAGWRQDDDVLDTWFSSALWPFSTLGWPADTPDLEKFYPTSVLLTGYDIIFFWVARMM
                     MFGIYAMADRGPADAVPFRTVALTGLVRDERGRKMSKSAGNGIDPLEWIETYGADATR
                     ITLARSASPGSDIPVSEELAQGGRNFVTKLWNATRFAIINGATVEGPLPDLATLP"
BASE COUNT          375 a          707 c          697 g          332 t
ORIGIN      
        1 cggcagggtc gcgagatctg gcagcggacc ttcgacggtc gcgccgttga tgatcgcgaa
       61 tctcgtggcg ttccacagct tcgtgacgaa gttgcggcca ccctgcgcga gctcttcgct
      121 caccggaatg tcggaccccg gcgaggcact gcgggccagc gtgatgcgag tggcgtcggc
      181 accgtaggtc tcgatccatt caaggggatc gatgccgtta cccgccgact tgctcatctt
      241 gcggccacgc tcgtcgcgaa cgagaccggt cagtgcaacg gttcgaaacg ggacggcgtc
      301 ggctggccct cgatcggcca ttgcgtagat gccgaacatc atcatgcgag cgacccagaa
      361 gaagatgatg tcgtacccgg tcagcaacac cgacgtcgga tagaacttct cgaggtctgg
      421 cgtgtcggcg ggccagccga gggtcgaaaa cggccacagc gccgaagaaa accaggtgtc
      481 gagcacgtcg tcgtcctgcc gccaaccagc agcggtcgga gcatcgtcgt cgggcccgat
      541 cacgaccgtc tcgccggcgg ggccgtacca gacgggaatg cggtgacccc accagagctg
      601 ccgagaaatg caccagtcgt gcatgttgtc gacccagtcg aaatagttgc gggccatctc
      661 gggcggatgg atcgtcacgc gaccgtcgcg cacggcgtcc cccgccgctt ttgccaacga
      721 ctcgaccttc acgaaccact gaagcgacag cctcggctcg atcaccgtgt cgcatcggga
      781 gcagtggccg accgagtgca cgtagggccg cttctcggcc acgatcaagc cgagttcgcg
      841 cagcgcggca gtgacggcag gccgcgcctc gaagcggtcg agcccttgga aaggcccgtg
      901 cgccgtgatg acgccgcgct cgtccatgat ggtgatcgac ggcagcccgt gccgctgccc
      961 gatctcgaag tcgttcgggt cgtgtgcggg cgtcaccttc acagcgccgg tcccgaactc
     1021 cggatcgacg tggtcgtcgg ccacgaccgg aatccgccgg cccgtcaaag gcaactcgat
     1081 ctcagtgccc accagatgtg cgtagcgctc gtcgtctgga tgcacagcga ccgcggtgtc
     1141 gccgagcatg gtctcgaccc gggtggtcgc caccgtgatc gggccgtagc tgatggagac
     1201 gagctccccc tcgtcgtccg aatgatcgac ttctatgtct gaaagcgcag tgaggcaccg
     1261 cgggcaccag ttgatgatgc gctcggcgcg gtagatcagg ccgtcgttgt agaggcgctt
     1321 gaagatcgtg ccgacggcgc gcgacgagcc ggcgtccatc gtgaagcgct cgcgcgacca
     1381 gtcgacgccg tcgcccagcc ggcgcatctg gctgaggatc cggccgccgt actcggcctt
     1441 ccactgccac acccgatcga caaatgcctc gcgcccgagg tcgtcgcgcg acacaccctc
     1501 cttgccgagt tcgcgctcga ccacgttctg ggtggcgatg ccggcgtgat ccatgcccgg
     1561 gagccacagc gcctcgtacc cctgcatgcg gcggcgcctg gtcacggcgt ccatcagggt
     1621 gtgctgcggg gcgtggccca tgtgcagctg gccggtgacg ttgggcggcg gcaacacgat
     1681 cgtgaacggc ggcttgccac tggcgacgtc agcggtgaaa tacccgcgct cgacccagcg
     1741 ctcgtagagc gtcgcctcga cggcggccgg cgcatagacc gaaggcaggt cggacgacga
     1801 cgggccggca gcgccggccg agccggcagc gtcggcagag ccagcagaat cggtcgcagt
     1861 cacaccgtcc agtctaagga tctcgagcaa gccgcttcgt cgagcaggcc gatccgttaa
     1921 gcgcagcgcc aaaacagccg aaacctacac agcgcccgct gggtgtgcac ccctgaggag
     1981 acggcgatgc ggggtaacca aacgacgcag gagatccctc agtcgtcgtt cgcgatgacg
     2041 atcttgcctg gcgccgctcc cgcacgtact cgtaaggtgc gcacacccaa agagaagcca
     2101 gccgcgcagt t
//
LOCUS       DASWVF010000214         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_235176, whole genome shotgun sequence.
ACCESSION   DASWVF010000214 DASWVF010000000
VERSION     DASWVF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235176"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1178
                     /locus_tag="VGD55_03935"
     CDS             42..1178
                     /locus_tag="VGD55_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0869524.1"
                     /translation="MNLGTRGVQEALDLLEYCNVSGGSKLSDLRRRNGAPELYGIRTW
                     CLGNEMDGPWQIGHRSAAQYGNLAAQTGRAMRLLDPKLTLIPCGSSGPDMPTFGAWEA
                     TVLQETYDVVDLISAHAYFREEDGDLASFLACPVRMDEFIDGVVATVDSVKASLKQTK
                     RIDIAFDEWNVWYQQAESSQATSDSWPFAPHLFEQDYNVADAVVVGGLLIALMRHSDR
                     VASANLAQLVNVIAPIMTEEHGRAWRQTTFYPFAQAAKMASGSVLMGKMVAPIVESAK
                     WGDVPMLDAVATHDPERGEMALFIVNRSIDREVSLRVQTYGALPDHIATATVLCDDDP
                     RRTAQAGSASILRSNHSAVRRDQGLEIVLPPVSWNVVKLVTDEV"
     gene            1246..2025
                     /locus_tag="VGD55_03940"
     CDS             1246..2025
                     /locus_tag="VGD55_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0869525.1"
                     /translation="MLFAIFFTVPLLWLVLATTKSGQQLETRAPLGWGSIHQLIGNWH
                     RLTAFEGDAYAGWFGNTVEYAGTALVIVLLVDIPAGYGLAISGVRVRRPLLLLTLVVM
                     LIPSFALVLPVFLELNDVHLLGNPLSLILPFSFFPFGVYLAYLHFSTATRQELLAAGR
                     IDGCTELQVFLRIALPLAKPVVALIAFFSFVANWNNYFLPLAMLPNSTEYPLQLGLVL
                     LPRNSPVLALGVLISILPVLLVFIVAQRYVARGLTAGSTTD"
     gene            complement(2044..2802)
                     /locus_tag="VGD55_03945"
     CDS             complement(2044..2802)
                     /locus_tag="VGD55_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0869526.1"
                     /translation="MTHPFDLTGKLAIVTGAKRGIGYAMAEALAAAGANIVGVSATIE
                     PEGSAIGAAVIAHGRSFEARAVDFADRDAVLALGSELEARAPDILVNNAGTIERAPAI
                     EHPLASWDRVLQIDLTSQFILSQALARPMIARGYGKVIFTASLLSFQGGINVPGYTAA
                     KSGVAGLTKALSNEWAHFGVNVNAIAPGYIATDNTQALRDDPVRNRSILDRIPAGRWG
                     QPSDLSGATVFLASAASDYVNGITLPVDGGWLGR"
     gene            complement(2799..3842)
                     /locus_tag="VGD55_03950"
     CDS             complement(2799..3842)
                     /locus_tag="VGD55_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0869527.1"
                     /translation="MLTAAYVGNRTVELQAAHVLPPSHGQVRIHVALVGLCGTDLHIV
                     HGSMDRRVQLPLIFGHEMSGTVDAVGGGVDGWVTGDKVTVMPLVWDDTCPACRAGNTH
                     ICQNLNFVGIDSPGALQPWWNVPASTLVRVPQHTELRDAALIEPVAVAVHDVRRGRVS
                     TGDKIIVIGGGPIGLIIATVARHAGGEVTVVELDPTRRALAAELGFIALHPEDFDDPA
                     WLNEWTSGAGADVVFEVSGAAAGIALALPLAKVRGTIVVVGIHPEPRSVDLQRIFWRE
                     LTIVGARVYEKSDFEAAVELVSSGVIPTRTLITRVVPLAETQAGLDELKAGNAMKVLV
                     DVRAGQPDDDGRS"
     gene            4010..>5101
                     /locus_tag="VGD55_03955"
     CDS             4010..>5101
                     /locus_tag="VGD55_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0869528.1"
                     /translation="MQRRVWLPVVVTALALAGCSSSKHNATTASSSPASASSSASGSA
                     PAPATTSSAAAPTSSAPAVGSASAPAPSSTASGNLTVWVDAVRLPDAKAYQAAHPNVK
                     MNIVTFDGDGNGATSLQTKIQLWNKAGSGWPDVIFSEQVNDPIWMASDPFNFAADMGA
                     ALGKSVTSQWPAPSLAQCTVNGTLVCIQDNLAPDVLWVNQKLMTQFGYTVPTTWQAWQ
                     ALGEKVATDHPGYIIGASGESYGAWQYFWANQCPLASNVSGTKVTINVNDVHCTEMAS
                     LLDPLIKDGTVPPLSVFSPDFAKKYGGAADKVLMMVGPAWYTGVFHDTLKTPAGELTA
                     AAPLQWGTDTPVTTGQIGGGPWIISKHSSN"
BASE COUNT          920 a         1624 c         1590 g          967 t
ORIGIN      
        1 tggcgtgggt caagcaggtt gggattgagc ccattgtcgc gttgaacctc ggaacgcgag
       61 gagtacagga ggcgctggat cttctcgaat attgcaacgt gtccggtggt agcaaactgt
      121 cggaccttcg tagacgcaat ggcgcgccgg aactgtacgg catccggacc tggtgcctcg
      181 gaaatgagat ggacggtccg tggcagattg gtcacaggtc cgcggcacag tacggcaacc
      241 tcgccgctca gaccggccgc gcgatgcgct tactggaccc caaactaacc ctgatcccct
      301 gcggcagttc agggccggac atgccgacat tcggcgcctg ggaagcgacg gtgcttcaag
      361 agacgtacga cgtcgtcgat ctgatttccg cgcacgccta cttccgtgag gaggatggcg
      421 atctcgctag ctttctagcg tgcccggtac ggatggacga gttcatcgac ggcgtcgtgg
      481 caacggtgga ctctgtcaag gcgtccctca agcagacgaa gcggattgat atcgcattcg
      541 atgaatggaa cgtgtggtac cagcaggcgg agagttcgca agcgacgtct gactcatggc
      601 cgttcgcacc acatctgttc gagcaggact acaacgtcgc cgacgctgtt gtagtcggcg
      661 gactcctcat cgcgttgatg cggcattcag acagggtggc ttcagcgaac ctggcccagt
      721 tggtcaacgt gatcgcgccc atcatgacgg aggagcatgg gcgtgcatgg agacagacga
      781 ctttttaccc tttcgcgcaa gctgccaaga tggcgtcggg ctccgtgctc atgggcaaga
      841 tggttgcgcc catcgttgaa tccgccaagt ggggagacgt gccgatgctt gacgcggtag
      901 ctacccacga cccggagcgc ggtgaaatgg cgctgtttat cgtcaaccgg tcgatcgatc
      961 gagaagtctc gttgagggtg caaacatacg gagcgctacc cgatcacatc gcaactgcga
     1021 ctgtgctgtg cgatgacgat ccacgccgaa cggcgcaggc gggatcggcg tcaatcctgc
     1081 gaagcaacca ctcggccgtg cggcgtgacc agggactgga aattgttctt cctcccgtgt
     1141 cgtggaacgt cgtcaaactg gtgaccgatg aggtctgagt cggcaactac gcccgcgcct
     1201 ttgacgagcc ggaggctggg tagcgggctg cgaacggtca tcttggtgct cttcgcaatc
     1261 ttcttcaccg tccccctgct ctggctcgtg ctagcgacca cgaaaagcgg tcagcaactc
     1321 gagacccgcg cgccgctcgg atgggggagc attcaccagc tcatcggcaa ctggcaccga
     1381 cttaccgcgt tcgaaggcga cgcctacgcc ggctggttcg gcaacacggt ggagtacgcg
     1441 ggcaccgccc tggtcatcgt gctgctcgtc gacatcccgg ccggctatgg cttggccata
     1501 agcggcgtgc gagtccgacg ccctctgcta ctcctgacgc tggtcgtcat gctcatccct
     1561 agtttcgcgc tggtactgcc ggtcttcctc gagctaaacg acgtgcacct gctcggcaac
     1621 ccgctatcgc ttatcctgcc gttttcgttc ttcccgttcg gggtctatct cgcctacctc
     1681 cacttctcga ccgcaacgcg gcaggaactg ctggctgccg gccgcataga cggctgcacg
     1741 gagctacagg tgttccttcg gatcgcgctc ccgctggcga agcccgtcgt cgcgctgatc
     1801 gccttcttca gcttcgtggc caactggaac aactactttc tgccgctggc gatgctgccg
     1861 aacagcaccg aatatccttt gcagctcggg ttggtcctgc tgcccaggaa cagcccggta
     1921 ctcgccctgg gagttctcat ctcgatcctg cccgtgctgc tcgtcttcat cgtcgctcag
     1981 cggtacgtcg cgagaggtct gaccgcggga agtacgaccg actaagagct agcgtcggtg
     2041 ccgctaccgc ccgagccacc cgccgtccac cggaagagtt atcccattga cgtagtcgga
     2101 cgccgctgaa gcaagaaaga ccgtcgcccc gctcagatca gatggttggc cccagcgtcc
     2161 ggccgggatg cggtcgagta tggagcgatt gcggactgga tcgtcacgca gtgcttgggt
     2221 gttgtccgtc gcgatgtatc ccggtgcgat cgcattgacg tttacgccga agtgcgccca
     2281 ctcgttcgat agcgctttcg tgagtccggc gacgcccgac ttggctgctg tatagcccgg
     2341 gacgttgatt ccgccctgaa agctcaacaa gctcgcggtg aaaatcactt tgccgtaccc
     2401 gcgcgcgatc atcgggcgag caagcgcctg gctcaggatg aactggcttg tcaggtcgat
     2461 ctggagaacc cggtcccagc ttgcaagcgg atgttcgatc gcaggagcgc gctcgattgt
     2521 gccggcgttg ttgacgagga tgtcgggcgc tcttgcctcc agctcggagc cgagggcgag
     2581 cacggcgtcg cggtcggcga agtcgacagc gcgcgcttca aacgaccgac cgtgcgctat
     2641 gactgcggcg ccaatcgcgc tgccctcggg ttcgatagtg gcgctgacgc cgacaatgtt
     2701 ggcgcctgcg gcggccaatg cttctgccat cgcatatccg atccctcgct tcgcgccggt
     2761 gacgatggcg agtttgccgg tcagatcgaa cgggtgcgtc atgacctgcc gtcgtcgtcc
     2821 ggctgcccgg cacggacatc gaccagcacc ttcatcgcgt tgccggcctt gagttcatcc
     2881 aacccagctt gcgtttcagc gagcggaacc acgcgcgtga tgagcgtgcg agtcgggatg
     2941 acgccagagc tgacgagttc gacggccgct tcgaagtcgg acttctcata tacgcgtgcc
     3001 cccacgatgg tcagttcgcg ccagaagatg cgttggagat ccacgctgcg aggctcgggg
     3061 tggatgccga cgacaacgat cgtgccacgt actttcgcca gcggtagcgc taacgctatg
     3121 ccggcggcgg cgcctgacac ctcgaacacg acgtcggcac ctgcgccgct cgtccactcg
     3181 ttgagccacg cgggatcgtc gaagtcttcg gggtgcagtg caatgaaacc gagttccgcc
     3241 gcaagtgctc gtcgcgttgg gtcgagctcc acgacggtga cttcgccgcc ggcatgccta
     3301 gcgaccgttg cgatgatcaa cccaatcggc ccaccaccaa tgacaatgat cttgtcacct
     3361 gtagacaccc gcccgcggcg tacgtcatgc acggcgacag ccaccggttc gatgagcgcg
     3421 gcatctcgca gttcggtgtg ctgcggcact ctcacgagcg tcgatgcggg aacgttccac
     3481 cacggttgca aggcgccggg ggagtcgatg ccgacgaagt tgaggttctg gcagatgtgg
     3541 gtgttgcctg cgcggcaggc ggggcacgtg tcgtcccaaa ccagcggcat gacagttacc
     3601 ttgtctccgg tcacccaccc atcgacgccg ccgccgaccg cgtcgaccgt gccgctcatc
     3661 tcatgtccga agatcaacgg cagctgcacc cgccggtcca tcgacccgtg aacgatgtgc
     3721 aggtccgtgc cgcaaagacc gacgagtgcg acgtggatac ggacctgccc gtggctggga
     3781 gggaggacgt gcgccgcctg gagctcgacg gtgcggtttc cgacatacgc ggcggtcagc
     3841 acggcgtctc cttgcgactt ggtatcgatt cggcttcgta ggttaccgcc tcattgccga
     3901 aacgtcacac gtatgatcta ctccgtccgg tcgccttttg tcctgtggtg acgacgaagt
     3961 ctcggtccct ggattctcga tcgaccctgt aagggagcaa gcagtgaaaa tgcagcgacg
     4021 tgtgtggctg ccggtcgtcg tgacggcatt ggctttggcg ggttgctcaa gttcgaagca
     4081 caacgcgacg accgcctcaa gttcgccagc tagtgcgtca tcgtcggcgt ccggctcggc
     4141 cccggcaccc gcgacgacca gttccgcagc ggccccaaca agttccgctc cggccgtcgg
     4201 cagcgcttcg gcgccggctc cgagcagcac ggcttcgggc aacctgaccg tctgggtcga
     4261 cgccgtgcga ttgccagacg ccaaggccta ccaagcggcc cacccgaacg taaagatgaa
     4321 catcgttacc ttcgacggtg acggcaacgg ggcgacgagc ttgcagacaa agatccaact
     4381 gtggaacaag gccggcagcg gctggccgga cgtcatcttc agcgagcaag tcaacgaccc
     4441 gatctggatg gcgtctgacc cgttcaactt cgccgcagac atgggtgccg cgctcggtaa
     4501 gagcgtcacc agccagtggc ccgcgccttc actcgcgcag tgcacagtca acggcacgct
     4561 cgtctgcatc caggacaacc tcgcccccga cgtcttgtgg gtcaaccaga agttgatgac
     4621 ccagttcggc tacacggtcc ccaccacgtg gcaggcctgg caggccctcg gtgagaaggt
     4681 cgcgaccgat cacccgggtt acatcatcgg agccagtggc gagtcgtacg gcgcctggca
     4741 gtacttctgg gccaaccagt gcccgctcgc gtcgaacgtc tccggcacca aggtgacgat
     4801 caacgtgaac gacgtgcact gcaccgagat ggcgagcctg ctcgacccgc tgatcaagga
     4861 cggtaccgtc ccaccgctga gcgtcttctc ccctgacttc gccaagaagt acggcggcgc
     4921 agccgacaag gtgctcatga tggtcgggcc ggcctggtac accggggtgt tccacgacac
     4981 gttgaagacg cccgctggag agttgaccgc cgcggcgcca ctgcagtggg gcacggacac
     5041 tccggtcacg accggccaga tcggtggcgg accatggatc atctccaagc actccagcaa
     5101 c
//
LOCUS       DASWVF010000215         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_336962, whole genome shotgun sequence.
ACCESSION   DASWVF010000215 DASWVF010000000
VERSION     DASWVF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336962"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..>1533)
                     /locus_tag="VGD55_03960"
     CDS             complement(152..>1533)
                     /locus_tag="VGD55_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0869529.1"
                     /translation="AKSWLDSIPDRAVRPRLSADEIAPSFAGPLPDGPTDPAEVIDLL
                     AALAEPGLMAMPGGRFFGWVIGGTLPAALAADWLTSAWDQNTGLRYATPASAAAEEAA
                     GGWILELLGLPAGADVGFVTGGTMANFSGLAAARYQVLAQAGWDVNTKGLNGAPPVRV
                     LVGAERHDTVDLTLAYLGLGEPTAVAADGQGRIRLDALEAALEEGSGPTIVCLQAGNV
                     HSGAFDPIGGATRLAHAHGEPGQRPWVHVDGAFGLWAAATPTLRQLVPGLAGADSWAT
                     DAHKTLNVPYDCGIVIVAQPTALRAALGVRASYLISAEGPAGDPDEKTPELSRRARGI
                     PVWAAIRSLGREGVADLVDGLVRHARAIAAGIETIEGAQVLNEVVFTQVCVAFDDDER
                     TKAVTAQLLADGTAWMSGSRWHGRDVLRVSVSNWSTDADDVEASVDAVRSAANAVRLA
                     SVGQPNTFG"
BASE COUNT          240 a          551 c          502 g          240 t
ORIGIN      
        1 agccgatcga ccgcctcttg tgcttcttcg tagctcccga aactggcgac tgtgttccag
       61 gccgcatgga ttgggtcgag ccgaattgca tctccggacg tcatcgtggc ctcctggtcg
      121 gactggcggt agcggtccca acggtggtgc gctacccaaa ggtattcggt tgtccaacgc
      181 ttgcaagccg caccgcgttc gcagcgctgc gcactgcgtc gaccgacgcc tcgacgtcgt
      241 ccgcgtcggt cgaccaattg ctgaccgaca cccgcagcac gtcgcgcccg tgccacctcg
      301 agcccgacat ccacgccgtc ccatcggcaa gcagctgtgc cgtgacggcc ttcgtgcgct
      361 cgtcgtcgtc gaaggcgacg cagacctgcg tgaagaccac ctcgttgagc acctgcgcgc
      421 cttcgatggt ctcgatcccg gcggctatcg cccgcgcgtg ccggaccagc ccatcgacga
      481 gatcggcaac gccctcccga cccagcgacc ggatggcggc ccacaccgga atgccgcgag
      541 cgcgccgtga caactccggc gtcttctcgt cgggatcgcc agccgggccc tcggccgata
      601 tcaaataact tgcccggacg ccgagtgctg cccgcagtgc cgtcggctgg gcgacgatga
      661 cgatgccgca gtcgtacggc acgttaagag tcttgtgcgc atcggtagcc cacgagtcgg
      721 ctcccgcgag acctggcacg agttggcgaa gggtcggtgt ggccgctgcc cacaagccga
      781 acgcaccgtc gacgtgcacc cacggtcgtt ggcccggctc cccgtgtgcg tgcgccaacc
      841 tggttgctcc accgatggga tcgaacgcgc cggaatgcac attgccggct tgcaggcaga
      901 cgatcgtcgg gccgctcccc tcctcgagcg ctgcttccag cgcgtccagg cggatccgac
      961 cctgcccatc ggctgctacc gccgtgggtt cgccgaggcc gaggtaagcc aaggtgaggt
     1021 cgacagtgtc gtgtcgttct gcgccgacga gaacgcgcac cggcggagcg ccattcaggc
     1081 ccttcgtgtt gacgtcccag ccggcctgcg cgagcacctg gtagcgggcg gcggcgagac
     1141 cggagaagtt cgccatcgtg ccgccggtga cgaagccgac atcagcgccc gccggcagcc
     1201 cgagcagttc gagaatccac ccacctgcag cctcttcggc cgcagcgctc gcgggcgtgg
     1261 cgtagcgcag gccagtgttc tggtcccacg cgctggtgag ccagtcggcc gcaagcgccg
     1321 ccggcaaggt gccgccgatc acccaaccga agaaccggcc gccgggcatc gccatcaggc
     1381 caggttcggc gagcgccgcg agcaggtcga tgacttccgc ggggtcggtg gggccatcgg
     1441 gaagcggccc ggcaaacgac ggcgcgatct catccgccga taagcgcggc cgtaccgcgc
     1501 ggtccggtat cgagtcgagc cacgacttcg cgt
//
LOCUS       DASWVF010000216         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_337049, whole genome shotgun sequence.
ACCESSION   DASWVF010000216 DASWVF010000000
VERSION     DASWVF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337049"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VGD55_03965"
     CDS             complement(<1..289)
                     /locus_tag="VGD55_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY0869530.1"
                     /translation="MPAVGDALLFGHVFLAPLIESFQCKRGQRLAWALRSSSANVRRG
                     GPDMADFVGAIDQGTTSTRFMVFDHAGIEIARHSLEHQQILPAAGYVEHNPV"
     gene            254..1156
                     /locus_tag="VGD55_03970"
     CDS             254..1156
                     /locus_tag="VGD55_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0869531.1"
                     /translation="MTEQQGIPDGWHPAPPPGSQRPYLYLPGEQPAGMPPVSQQPPTK
                     TKRRRWERAGGGVAAGGGVAAKAGLLTKLLVLLKGSAVLIKFKFVLSMAVSVIAYAFI
                     FGWWYAVGFVALIAVHEVGHLVVLRSMGVKVSMPTFIPFMGAFVKMQSKPRSVAQEAI
                     GALAGPAVGTLGALGALELSNVFNSPLLRALAYTGFFLNLFNLVPALPLDGGRVAGAL
                     HPALWIVGLVGAGIFAILHPSPVLIFILVLGIFELARRWRTHKWIDAAYYAIPPSTRV
                     GIGAAYAAVAVMCLWGMSLAYVPR"
     gene            1228..>2611
                     /locus_tag="VGD55_03975"
     CDS             1228..>2611
                     /locus_tag="VGD55_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0869532.1"
                     /translation="MGDAETELEREQRYVDRLYGRLDELRDRAERELIAVRATAPSGT
                     HQNRSERDAFATLYEQRLSQLRAVEDRLVFGRFDTVEAGRRYVGRIGLSDEDRHQLLI
                     DWRAPAARLFYQATAAAPGDVVRRRHLTTRDRAVTAIEDEVLDLDGLEAGDLSTLSGE
                     GALLAALNAKRTGRMGDIVATIQAEQDDVIRAELPGVLVVQGGPGTGKTAVALHRAAY
                     LLYTHRERLARSGVLLVGPNQTFLRYIEKVLPSLGETGVVMVTPGELLPGVVATTDDA
                     AEVVALKGDVRMAGVIAAAVEQRQRVPDATRTLNVSGHELLLRPHVVAAARAHARRGG
                     KPHNLARVSYVKEILDGLASQYARQLGVQLSVDNRAELLADLRDAPDVRREVNLTWMP
                     MTPDGLVEGLFADPWRLAAAAPYLTDAERALLRRDRGARWTISDIPLLDEAAELLGDD
                     DTGGYELKRQE"
BASE COUNT          407 a          847 c          897 g          460 t
ORIGIN      
        1 cgacggggtt gtgctcgacg tagcccgccg ccgggaggat ctgttgatgc tcgaggctgt
       61 gccgcgcgat ctcgatgccc gcatggtcga agaccatgaa ccgcgtgctg gtggtgccct
      121 ggtcgatcgc gccgacgaaa tcagccatgt ctggcccgcc tctcctcacg ttcgctgacg
      181 acgaacgtaa ggcccacgcg agccgttgcc cacgcttaca ctgaaagctc tcgatgagtg
      241 gggctagaaa cacgtgaccg aacagcaagg catccccgac ggctggcatc ccgcgccgcc
      301 gccgggatcg caacgtccct acctttacct gcctggcgag caacccgccg gcatgccgcc
      361 cgtgagccag cagcctccaa caaagaccaa gcggcggcgg tgggagcgcg cgggcggtgg
      421 ggtcgccgcg ggcggcggag tcgcggcgaa ggccggactg ttgaccaagc tgctcgtgtt
      481 gctcaagggc agcgccgtgc tcatcaagtt caagttcgtg ctgagcatgg ccgtttcggt
      541 catcgcctac gcgttcatct tcggctggtg gtacgcagtc gggttcgtcg cgctgatcgc
      601 cgtgcacgaa gtcggccacc tcgtcgtgct gcggtcgatg ggtgtcaagg tgtcgatgcc
      661 gacgttcatc ccgttcatgg gcgcgttcgt caagatgcag tcgaagccga ggtcggtcgc
      721 gcaggaggcg atcggcgctc tcgccggacc tgcggtcggc actcttggtg cgctcggtgc
      781 gctcgagctc tcgaacgtgt tcaactcgcc actgctgcga gcgctcgcct acaccggctt
      841 cttcctcaac ctgttcaacc tcgtacccgc attgccactc gacggtgggc gcgtggcggg
      901 cgccttgcat ccagcgctgt ggatcgtcgg tcttgtcggc gccggaatct tcgcgatctt
      961 gcacccgagc ccggtgctca tcttcatcct ggtgctcggc atcttcgagc tggcgcggcg
     1021 atggagaacc cacaagtgga ttgacgccgc gtactacgcg atcccgccgt cgacgcgggt
     1081 tggcatcggt gccgcctatg cggccgtcgc cgtgatgtgc ctgtggggca tgagcctcgc
     1141 gtacgtccct cgctgagcac ccattgcggg gccccgatag ggtagacgcc gcacgatcgg
     1201 ggctaactcg tgctcggaag gggttcggtg ggcgacgcgg agaccgaact cgaacgcgag
     1261 cagcgctacg tcgatcgctt gtacggcagg ctcgatgagc tgcgcgaccg cgccgaacgc
     1321 gagctgatcg ccgtccgggc gactgcgcct tctggcactc accagaaccg ctccgaacgc
     1381 gacgccttcg cgacgctgta cgagcaacgg ctctcacagt tgcgcgccgt cgaagaccgg
     1441 ctggtgttcg ggcgtttcga cacggtcgag gccggacgcc gatatgtcgg gcgcattgga
     1501 ttgtctgacg aggatcgcca tcaactgctc atcgactggc gcgctccggc agcgcggctg
     1561 ttctaccagg cgaccgcggc tgcgccgggg gacgtggtgc gtcggcgcca tctgacgacg
     1621 cgcgaccgtg cggtgacggc gatcgaggat gaggtgcttg atctcgacgg tctggaagcc
     1681 ggcgacctct cgacgctgtc gggtgaaggc gcgttgctcg ccgcgctgaa cgcgaaacgc
     1741 accggtcgca tgggcgacat cgtcgccacg atccaagccg agcaggacga cgtcatcagg
     1801 gctgaattgc ctggagttct cgtcgtgcag ggcgggccgg gtactggcaa gaccgcggtc
     1861 gcgctgcacc gcgcggcgta cctgctctac acccaccgcg agcggctggc ccgtagcggt
     1921 gtgctgctgg ttggacctaa ccagacgttc ttgcgctaca tcgagaaagt actgccttca
     1981 cttggcgaga ccggtgtcgt gatggtgacg ccgggtgagt tgctgcccgg tgtggtcgcg
     2041 accaccgacg acgccgccga ggtggtcgcg ctgaagggcg acgtgcgcat ggccggggtg
     2101 atcgcggcgg cggtcgagca gcggcaacgg gtgcccgatg cgacgcgcac gttgaacgtc
     2161 agcggtcacg aactgctgct gcgcccgcat gtcgtcgcgg ccgcgcgggc gcacgcacgg
     2221 cgaggcggca agccgcacaa cctcgcacgg gtgtcctacg tgaaggaaat tctcgacggc
     2281 ctcgcctcgc aatatgcccg gcaactcggc gtccaacttt cggtcgacaa ccgagctgag
     2341 ctgctcgcag acctgcgcga tgcacccgac gtgcgacgcg aagtgaacct gacctggatg
     2401 ccaatgaccc ccgacggcct ggtcgagggg ttgtttgccg acccttggcg gctcgcggct
     2461 gcagcccctt atctcaccga cgcggagcgg gcgctattgc gtcgtgaccg cggcgcgcgt
     2521 tggacgatct ccgacatccc gttgcttgac gaggcggccg aattgctggg tgacgacgac
     2581 accggtggct acgagctgaa gcggcaagag g
//
LOCUS       DASWVF010000217         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439162, whole genome shotgun sequence.
ACCESSION   DASWVF010000217 DASWVF010000000
VERSION     DASWVF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439162"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..383
                     /locus_tag="VGD55_03980"
     CDS             93..383
                     /locus_tag="VGD55_03980"
                     /inference="COORDINATES: protein motif:HMM:NF041681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HEY0869533.1"
                     /translation="MADRPLDDLGIDEHFGHIQARPVSWVVVFLVCAGFLVAGIGLIA
                     ANPVVFWIGVGVVAAGTILGGVTHAMADVPSRVERRQAKANKVAVVDSEPVR"
     gene            492..1664
                     /locus_tag="VGD55_03985"
     CDS             492..1664
                     /locus_tag="VGD55_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEY0869534.1"
                     /translation="MIACPDAFGMLAEGRRVVFVSATNGKTTTTKLLAAAMRAAVPVV
                     TNASGANMPAGIVAALARPPVGVPADALAVIEADETYLPGLLPRTEKPLLVLGNLTRD
                     QLDRVGEVAMVARRWRTIFAATKGLSAVANADDPHVVWAASVIPDVTWVSVGQRWTAD
                     SAVCPACGSILHREADDGHHPDWSCTCGFRRPETSWSVDSGELLGPDGERYSLDLQLP
                     GAANQANAAMAVVAANVLGVPPSVSLPALRTVASVGGRYQTVSLGGRQVRLLLGKNPA
                     GWSEMFGMLAPAPAPVVIAINARAADGKDPSWLWDVPFERLADREVIATGDRRHDLAV
                     RLMYADVSHTTVADPWISGGLPFDAGLPGRPAIDAVGTYTAFRELLHRGGGADDGN"
     gene            1654..>1866
                     /locus_tag="VGD55_03990"
     CDS             1654..>1866
                     /locus_tag="VGD55_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0869535.1"
                     /translation="MATESRLRLVSIYPVLLGTYGDGGNLRVLAMRAKARGFDVEAIE
                     LDAGEPVPDSADLYVLGGGEDDAQVAA"
BASE COUNT          284 a          594 c          642 g          346 t
ORIGIN      
        1 gcccggtaag cccgaaggcg aagtcgacac ccggccatga cgcacactga tactcgtcga
       61 cctgctgtta tccgataagg agatcgcgtc ccatggctga ccgaccgcta gacgatctcg
      121 ggatcgacga gcacttcggg cacattcagg ccaggcccgt gtcgtgggtc gtggtgttcc
      181 tggtgtgcgc aggcttcctg gtggccggta tcggcctgat cgcggcaaac cccgtggtgt
      241 tctggatcgg cgtcggtgtc gtggccgctg gcaccatcct cggcggtgtt acgcacgcta
      301 tggccgacgt accgtctcga gtcgagaggc gtcaggccaa agccaacaag gtcgccgtcg
      361 tcgacagtga accggtgcgt taactgagct cccttcggct gcggacggcg attggtctcg
      421 gcacgatcgc cggcagactt tcgaagtctc tcggtcgcgg tgacggtctc gtcatcggcg
      481 gacgcgtcgc gatgatcgcg tgtcccgacg cattcggcat gcttgccgaa ggccgtcggg
      541 tcgtcttcgt ttcggccacg aatggcaaga caacaacgac aaaattgctc gctgccgcca
      601 tgcgcgccgc ggtgcccgtg gtgacgaacg cctcgggtgc aaacatgccg gccgggatcg
      661 tcgccgctct tgctcgcccg ccggtcggcg tcccggctga cgcacttgcc gtgatcgaag
      721 ccgatgagac gtaccttcct ggcctgctgc cacgaaccga aaagccgttg ctcgtgctcg
      781 gaaatctgac tcgcgaccaa ctcgaccggg tcggtgaggt ggcgatggtc gcgcggcgat
      841 ggcggactat tttcgctgct accaaaggac tttcggcggt cgccaatgcc gatgacccac
      901 atgtcgtgtg ggcggcgagc gtgatccccg acgtaacctg ggtgtcggtt ggtcagcggt
      961 ggacggctga ctctgcggtc tgtccggcat gtgggtcgat cctgcaccgc gaggccgacg
     1021 acgggcacca tccagactgg agctgcacgt gtgggtttcg gcggcctgaa acgagctggt
     1081 cggtcgactc gggcgaactc ctcgggccag acggcgaacg ttactcactc gacctgcagt
     1141 tacccggtgc cgcgaaccag gcgaacgcgg cgatggccgt ggttgccgcc aacgtgcttg
     1201 gcgtgccgcc gtccgtctca ttgccggctc tgcgcactgt cgcgtctgtc ggtggtcgct
     1261 atcagaccgt gtcgctcggc ggccggcagg tacggctgct gctgggaaag aatccggcag
     1321 gttggtcgga gatgttcggc atgctggcac ctgcgccggc gccggtcgtg atcgccatca
     1381 acgcgcgcgc ggcggatggc aaggatccct cgtggctgtg ggacgtaccg ttcgaacggc
     1441 tcgccgatcg tgaagttatc gcgaccggcg accggcggca cgacctcgcc gtccggctga
     1501 tgtacgccga tgtctcccac acgacggtgg ccgacccgtg gatcagcggt gggttgccgt
     1561 tcgacgccgg gctgccgggt cgacccgcta tcgatgccgt gggcacctac accgcgttcc
     1621 gggaactcct gcatcgcggg ggaggggctg acgatggcaa ctgagtcgcg acttcgactc
     1681 gtctcgatct acccggtcct ccttggcacc tacggcgacg gcggcaatct gcgggttctc
     1741 gcgatgcgcg cgaaggcacg cggcttcgac gtcgaagcca tcgagctgga cgccggggag
     1801 ccggtgcccg atagcgccga tctgtacgtg ctcggcggcg gcgaagacga tgcgcaggtc
     1861 gcggcc
//
LOCUS       DASWVF010000218         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_311547, whole genome shotgun sequence.
ACCESSION   DASWVF010000218 DASWVF010000000
VERSION     DASWVF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311547"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1046)
                     /gene="tsf"
                     /locus_tag="VGD55_03995"
     CDS             complement(213..1046)
                     /gene="tsf"
                     /locus_tag="VGD55_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0869536.1"
                     /translation="MANYTTADVKRLRELTAAGMMDCKKALDETDGDFDKAVELLRIK
                     GAKDVGKRAERTTVNGLIGAQATGSSGVLLELKSETDFVAKGDRFQQLGATLVKTFAE
                     SDATDAEALLGAEVDGRKVSELIDESSAALGEKIELGRVAKYTGGGHVVAYLHKTSPD
                     LPPQLGVLIELDSEHPEVGKDVAQHIAAFSPTYLHREHVPADVVENERRIAEATAREE
                     GKPEAALPRIVEGRVTGFYKDAVLVEQAFAKDSKKSVQQVLDEAGVKAVRFARFRVGQ
                     S"
     gene            complement(1121..2059)
                     /gene="rpsB"
                     /locus_tag="VGD55_04000"
     CDS             complement(1121..2059)
                     /gene="rpsB"
                     /locus_tag="VGD55_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0869537.1"
                     /translation="MAVVTMKELLSSGVHFGHQTRRWNPKMKRFIFTERNGIYIIDLQ
                     QSLTYIDRAYEFVKDTVAHGGSVLFVGTKKQAQEAVAEQATRVGMPYVNQRWLGGMLT
                     NFQTVYKRLQRLKELEILEQGGASYGQRTKKEALVLQREKDKLLKTLGGIREMSRVPS
                     AVWIVDTKKEHIAVGEARKLGIPVVAILDTNCDPDEVDYKIPGNDDAIRSVSLLTRVV
                     ADAVAEGLMARVAARVGDAKPDVDAGSELGGGEPLADWERELLVRNTAAADAAAEAAT
                     EAAAVAAPADIAAEVPAAVETPATVEPEAPAADAEL"
BASE COUNT          388 a          878 c          825 g          481 t
ORIGIN      
        1 atgccgtctc cggcgaacgc ttcgccggac agcttgagca cgacccgctg gtaaccctgg
       61 tagggctgga cgccgtcccc ccgcgcgacc acgccgccga actcgacttc accagaactg
      121 gtcaacgagc gcccctcatc aatggtcggc cgcctcaaat cgcccgtgaa cgccatgcgt
      181 gcctaccgtg ccggtgctgg ctgaagtctg cctcaggatt gccccacgcg gaaccgagca
      241 aagcggaccg ccttgacgcc cgcctcgtcg agcacctgct gcaccgactt cttgctgtcc
      301 ttcgcgaacg cctgctcgac cagcacggcg tccttgtaga agccagtcac ccgaccctcg
      361 acgatcctgg gcagcgcagc ctcgggcttg ccctcttcgc gagcggtcgc ctcggcaata
      421 cggcgctcgt tctcgacgac gtcagccggc acgtgctccc ggtgcagata ggtcggcgag
      481 aaggccgcga tgtgctgagc gacatccttg ccgacctctg ggtgctcact gtcgagctcg
      541 atcaggacgc cgagttgtgg cggcaagtcc gggctcgtct tgtgcaggta ggccacgaca
      601 tgaccgccgc cggtgtactt cgccacccgg ccgagctcga tcttctcgcc caaggcggca
      661 ctgctctcgt cgatcaactc gctgaccttg cggccatcga cctcagcgcc gagcagggcc
      721 tccgcatcgg tcgcgtcaga ctccgcgaac gtcttcacca gggtcgcgcc gagctgctgg
      781 aagcgatcac ccttggcgac gaagtcggtc tcgctcttca actcgagcag gacgccggaa
      841 ctgccggtcg cctgcgcgcc gatcagcccg ttgacggtgg tgcgctctgc ccgcttgccg
      901 acgtccttcg cacccttgat gcgaagcagc tcgacggcct tgtcgaaatc gccgtcggtc
      961 tcgtcgagcg ccttcttgca atccatcatg ccggcggcgg tgagctcacg gagccgcttg
     1021 acgtcggccg tggtgtagtt cgccatgatc gctttcgcgt ccttagatgt tggtagttcg
     1081 ctagtagggg cgaggttggt ggttcggtag gagcggcgac tcagagttcg gcgtcagccg
     1141 caggtgcttc gggttcgacg gtcgcgggcg tttcgacagc cgcgggcact tcggcagcga
     1201 tatctgcagg cgcagcaacc gctgccgctt cagtagccgc ttctgcagca gcgtcagcag
     1261 ccgctgtatt ccgcaccaga agctcacgct cccagtcggc gagcggctca ccgccaccaa
     1321 gctcgctgcc ggcgtcgaca tcaggcttgg cgtcgcccac gcgagccgcg acgcgagcca
     1381 tcagaccctc ggccactgcg tcggcgacca cgcgggtgag caggctcacc gaacggatcg
     1441 cgtcgtcgtt gcccgggatc ttgtagtcga cctcgtcggg gtcgcagttg gtgtcgagga
     1501 tcgcgacgac cgggatgccg agcttgcggg cttcgccgac ggcgatgtgc tctttcttgg
     1561 tgtcgacgat ccacaccgcg ctcggcactc gcgacatctc gcggatgccg cccagcgtct
     1621 tcagcagctt gtccttctcg cgctgcagaa cgagggcttc cttcttggtg cgctggccgt
     1681 agctcgcgcc gccctgctcg agaatctcga gctccttgag gcgctgcagc cgcttgtaaa
     1741 cggtctggaa gttcgtgagc atgccgccga gccagcgctg gttgacgtag ggcatgccga
     1801 cgcgagtcgc ctgctcagcg actgcttcct gggcctgctt cttggtgcca acgaacagca
     1861 cggagccgcc gtgcgcgacg gtgtccttga cgaactcgta ggcgcggtcg atgtaggtca
     1921 gcgactgctg gaggtcgatg atgtaaatgc cattgcgctc ggtgaagatg aatcgcttca
     1981 tcttcgggtt ccagcgccgg gtctgatgcc cgaaatggac gccgctcgaa agcagctcct
     2041 tcatggtgac gacggccatg gccgcgcact ccttctggca ccccacatct tgccgcacgt
     2101 tcgcgggttg acgtcgccgg gcgccgatcc tcggttcttc gcggacgccg gtcggcggcc
     2161 acgcctgacg ccctgatcgc cgtgacacct cgatctaggg gacctagggc cgaaggcacc
     2221 gagtggcagc cgaccctgag cacctgtaaa ggagccgggg cggtggtagg gcgtgcgatg
     2281 taggccgggc ggacccgacc tcgttggcga gtctacgcga gcgtcgccga gcgtgaccaa
     2341 tcaccgccgt cagtagccgt cagcgggagt tgtccacagc agcccgccga gcgctcgcgt
     2401 cgtacaccga agcaggtgac gaggtgtcct gcgctccccg gaacccccaa gctccaggca
     2461 tggctacccc tcgatggtcc tggcttgctg ggctgctgac gaccaccttc atgttgggtg
     2521 tcggtgctcc ctcaagagcg gcggcgggct ctggccgcgg gtttgctgcg cc
//
LOCUS       DASWVF010000219         8532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_158838, whole genome shotgun sequence.
ACCESSION   DASWVF010000219 DASWVF010000000
VERSION     DASWVF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158838"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..1961
                     /locus_tag="VGD55_04005"
     CDS             399..1961
                     /locus_tag="VGD55_04005"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0869538.1"
                     /translation="MRRGLRPASALVVCICLVLSVALAWAAHVVDVRSNHRLLVREVQ
                     QAGAALSSTLPVVQSQLVDAVQVAIDTNANPQVFQRFVAASPSSTGSTSAISLWQISD
                     GTAKQIALQGKPLALVSAHLDAAFFARVHPSAQLFVTGIIPGTPARLGYAEMPLGDTS
                     GLVVYREAALPTQHIVIPASSPFAGLRVALYLGPKTVNPQLLEATDATPIHGRTASTS
                     LPFGDTVITVVGASKSPLAGGLSGALPWIVLGVGAVVALVSASVVESSIRRRLLAEGL
                     AQDNERLYRQQRTIAGTLQHALLPQVPDIDGVEIAARYSAGVADLEVGGDWYDVIRTE
                     NGRCVFFVGDVSGRGLQAATTMAALRYAARAYTAQGDDIETVLMKLGTLLEVENDRQF
                     ATVLAGEIDLRRNRVSVASAGHFMPLLLADGDATYIVAPVAPPVGVGSLTSVPTVTFD
                     VPPGARLIAFTDGLIERRDDASIDTGLERLREAAANHEAGLEDLLDTLMSVLVSETAS
                     DDTVILGMRWRA"
     gene            complement(1981..2364)
                     /locus_tag="VGD55_04010"
     CDS             complement(1981..2364)
                     /locus_tag="VGD55_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0869539.1"
                     /translation="MRSHTSADGRVSPSQLRALLLIDEAGPLPVTQLADEMGAMVSSA
                     SRLCDRLVAAGLVERSASPANRRVTLIRLTSPGTRLLASMRDARLTELGAVMSGMSAT
                     ERNGLLHGLLAIREADEQRRLRSHG"
     gene            2508..3662
                     /locus_tag="VGD55_04015"
     CDS             2508..3662
                     /locus_tag="VGD55_04015"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0869540.1"
                     /translation="MPRITMIQAFPFAAQLMRSASPGSLFTSLRAALASDGLTARLWL
                     ADYRQVRLRSVDDTDGSSEQVSIADGAIGRVYASQRHAVVIARHGSIAYLPLHARGER
                     LGVLQVTANSALGAEELAQLDDIATALASELIAALRMTDHFERPRRNKALTVAAEMQW
                     ALLPPTAYHDDRVSLAGLVEPAYSVAGDAFDWSVARETVTIAVFDGVARGVTAALATA
                     LCVNALRNARRAPVPLADQVALADQALYAHFGGKSHISTLVVQLDLRSGSAVAIDAGS
                     PQLLVVRDRQVNTVVLEPQLPLGMFEETHYREQPLTFVPGDRLIIVSDGVHAAGEPIA
                     FGVTALREAIDSTVESSPPETVRQLIAALHVHHGDELEDDAVVLCLDWDA"
     gene            complement(3670..4152)
                     /locus_tag="VGD55_04020"
     CDS             complement(3670..4152)
                     /locus_tag="VGD55_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0869541.1"
                     /translation="MGDAQEDRAKAAKLPEGDIVRILLTQHAEVKDMLDQIEAAAGAE
                     RKKLFTKLTTTLKAHETAEESVVRPVTKETAGEDVVDARNAEESEADDLIAALSDLDV
                     DGATFDAQFAEFKQAVSDHAEAEEKEEFPTIQSGRNAAERKALGDEFLAQYAAAGGKA
                     "
     gene            complement(4189..4404)
                     /locus_tag="VGD55_04025"
     CDS             complement(4189..4404)
                     /locus_tag="VGD55_04025"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY0869542.1"
                     /translation="MAKDLSSTKERKKLSSKSFALPGKRKYPIPDKAHARNALARVAQ
                     KGSPAERKTVRAAVKKRFPSIGKKKNS"
     gene            complement(4676..6178)
                     /locus_tag="VGD55_04030"
     CDS             complement(4676..6178)
                     /locus_tag="VGD55_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0869543.1"
                     /translation="MDHRLRAVCDLFMGDVREAAGLHEYDGLIQDLSPHGVRANLAKL
                     GGAPLNDTHDEAHLRAFEDNARLIFGKLELHRSNPVLHLANLDVSCYDREYAPAEQRA
                     AAKSAHVAAWPDAIDMTIETLDAVAAPVATSLLGAFNGLAAAIDAADPHAPAALAAHA
                     RLMSHLHDAAENGRPEAALGAAGLAALMATVEATTVDVERLAELADTERDRQTAILRD
                     ACKHIDPDAELPDVVAKLMADHPDADGVLSEAQAQVDEVLAFTREHDLVPYTDGACLV
                     ATAPESRRWATAMMAWAAPEEADAPSFYWVTPPEPTWPPNEIEEWLQVFSHATLPGIT
                     LHEVSPGHFAHSRAMRRAPTPVRRILMSPAFVEGWAHYTEELCLDLGFRSGDPRFVAG
                     IAIEALMRVTRLAAAIGVHTGAMTVDDAARRFESDAFASGRAARAEAARATFDPTYGR
                     YTWGKMIIRETQSKARDAWGTGYSHSRFHAAMLDLGAPPLGLLGTSIERG"
     gene            6280..7080
                     /locus_tag="VGD55_04035"
     CDS             6280..7080
                     /locus_tag="VGD55_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0869544.1"
                     /translation="MAGGLALRIVVCVKQVPDTWSEKRLDPADQTLDRAAADGVIDEL
                     DTYAVEEALRIKESNEGSTVVVLTMGPAGAADTLRKALSMGADEAVHVVDDALHGSDA
                     LATSLVLAKAIERTGFDLVVLGSESTDARMGVVPAMLAERLGVPQASLASKVEVESGT
                     VRVHRQTETGVAVVSADLPAVVSVLEKINEPRYPSFKGIMAAKTKPTATWSLADLGID
                     ASAVGLAAAATQVIDATQRPPRGDRTIIKDDGDAGARIADFLVSRKLL"
     gene            7094..8041
                     /locus_tag="VGD55_04040"
     CDS             7094..8041
                     /locus_tag="VGD55_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0869545.1"
                     /translation="MADVLVLVDDVGVGKPTLEMLTLAARLGSPVAVLLSGGADVEKL
                     AQYGATAVLVASDDQLTSVVVAPAVSLLESLVATRSPRAVLIASTAEGKEIAGRLGVR
                     LDAGVLTDAVDVALDGDAVVVEQSVFGGSTLVHSRVTRGVPIITVRPNSITAVVAQVT
                     ADVSVVSVPALAGRHAVVASRSTESAGGRPGLSEATVVVAGGRGVGAADKFALIEELA
                     DAMSAAVGASRAATDAGWYPHAAQVGQTGVTVAPQLYIACGISGAIQHRAGMQTSKTI
                     VAINKDAEAPIFELADFGVVGDLHVVIPQLIAEIARRKG"
     gene            complement(8106..>8532)
                     /locus_tag="VGD55_04045"
     CDS             complement(8106..>8532)
                     /locus_tag="VGD55_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0869546.1"
                     /translation="IAGAEVVVLAVPGGAVGEVASELGAALDGKVVVDATNHIGAAAV
                     NSRAEIGAAAPGAHYVRAFNTLGWENFASPLPGADLFFAADPAARETAEVLIAAVGLR
                     PAYVGDAESVATVDALLPLWFALVKQHGGNRKVALRVVD"
BASE COUNT         1350 a         2815 c         2803 g         1564 t
ORIGIN      
        1 gcgtgaagcg tgtccgcggc tgaagcgggg cttcccagcc ccacagagcc ttttgatgca
       61 tggctacgcg cgactactgc gctacgcgcc gcggaggtgc gaaacgcacc gcacgcagag
      121 gtggtgcggc tggccgagga cgtaatccga gcccggaacg ccttgaccat ggaccgcgta
      181 caggctggct gggagccgcg cgacgacatc ctccggcatc tcagcgccga cgagttccta
      241 ctccgcgaac gcgacgacgc tgattggccg ccgcaaactc gggacggaca agcgtgactg
      301 aaacccgcgc gtcctccatc tctacgttcc aacgcccgcc cgcgccgccg ccaacgtctc
      361 gacccgcgga cttcggcccg ctcgaagaca ccgatggggt gaggcgtggc ctgcgtccgg
      421 ccagcgcgct cgtggtgtgc atctgtctcg tactctccgt tgctctggcg tgggcggcac
      481 acgtcgtcga cgtgcgcagc aaccaccgtt tgctcgtgcg ggaggtccaa caggccggcg
      541 cggcgttgtc gagcacgctg cccgtcgtcc agtcgcagtt agtggatgcc gtccaggttg
      601 cgatcgacac aaacgcaaac ccgcaggttt tccaacgatt cgtcgctgcg tcgccttcat
      661 cgactggctc cacgtccgcg atctcgctgt ggcaaatcag tgatggaact gccaaacaga
      721 tcgcccttca gggcaaaccg ttggcgttgg tgtcagcgca ccttgatgcg gccttctttg
      781 ctcgcgttca ccccagtgca cagttgttcg tgaccggcat catccccggt actcccgccc
      841 ggctggggta tgccgagatg cccctcggcg atacgagcgg cctcgtcgtg taccgcgagg
      901 ccgccttgcc gacacagcac atcgtgattc ccgcctcgtc gccgtttgcc ggactccgag
      961 ttgcccttta tctaggaccc aaaaccgtga acccgcagtt gctcgaagcg accgacgcga
     1021 cgccgatcca cgggcgaacg gcatcgacct cgctgccgtt cggtgacacg gtcatcaccg
     1081 tggtcggcgc gtcgaagtcg ccactcgcgg gaggtttgtc gggcgccttg ccatggatcg
     1141 ttctcggtgt cggcgcggtc gtagcgctgg tcagtgcgtc tgtcgtcgag tcttcgatcc
     1201 ggcggcggtt gctcgcagag ggcttggcgc aagacaacga gcgcctgtac cggcagcaac
     1261 gcaccatcgc gggcaccttg caacatgcat tgctcccgca ggtgccggat atcgacggtg
     1321 tcgagattgc cgcccgttac tcggcggggg tagccgacct cgaggtcggc ggtgactggt
     1381 acgacgtcat tcgcacggag aacggacgct gcgtgttctt cgttggtgac gtgtcgggtc
     1441 gcggcctgca ggctgcgacc acgatggccg cgttgcgcta tgcggcgcgt gcctatacgg
     1501 cgcaaggcga cgatatcgag actgttctga tgaaactcgg cacgctcctc gaggtcgaga
     1561 acgatcgcca gttcgccacg gtgctggccg gtgagatcga ccttcggcgt aaccgggtga
     1621 gcgtcgcttc ggcgggccac ttcatgccgc tcctgctcgc tgacggtgac gccacgtaca
     1681 ttgtcgcccc ggtcgcgccg cctgttggtg tcggatcgct gacgagcgtg ccgacggtga
     1741 cgttcgacgt tccgcctggc gcacggttga tcgccttcac cgacggcctc atcgaacgcc
     1801 gagacgacgc gtcgatcgac accggtctag aacgactccg cgaggctgcg gccaatcacg
     1861 aagcgggcct cgaagacctg ctggatacct tgatgtctgt ccttgtcagt gagaccgcga
     1921 gcgacgacac ggtaatcctg ggcatgcgat ggagggctta gctaccgcgt gataccgcca
     1981 ttatccgtgg ctgcgcagcc gacgctgctc gtcggcttcg cgaatcgcga ggagtccgtg
     2041 cagcaggccg ttccgttcgg tcgccgacat gccactcatc acggcgccca gctcggtcag
     2101 tcgagcgtcc ctcatgctcg cgagcaatcg ggtacccggc gacgtcagac gaatgagcgt
     2161 gacccggcga ttcgccgggc tcgcgctgcg ctcgaccagg ccagcggcca ccaggcggtc
     2221 acacagcctg ctcgccgacg acaccatggc ccccatctcg tcggccagct gggtgactgg
     2281 cagcggccct gcctcgtcga tcaacagcaa agcgcgcagc tgggagggcg aaacccgccc
     2341 gtctgccgac gtgtgcgatc gcaaccacag cgagacaaac gcctgcgctg cgtcgtggac
     2401 cgcttcgaca tcggcggatg cttggtggcg cgagttctgg gcgatcctcg actcctttcg
     2461 cgtgcgctca agaaccccgg cgcgctctcc aacaccaacg gatcaccgtg ccgaggatca
     2521 cgatgattca ggcctttccc ttcgccgcgc aactcatgcg ttcggcgtcg cccggaagcc
     2581 tcttcaccag ccttcgggcc gcgctcgcat ccgacggcct gacggccagg ctttggctcg
     2641 ccgactatcg acaggtcagg ctgcgctcgg ttgacgacac cgacggctcg tcggagcagg
     2701 tttcgatagc cgacggtgcg attgggcggg tttacgcgag ccagcggcat gccgttgtga
     2761 ttgcgcgtca cggctccatc gcgtatctgc ccctccacgc tcgcggcgag cgcctcggcg
     2821 tccttcaggt gacggcgaac tccgcgctcg gagcggaaga actcgcccaa ctcgacgaca
     2881 tcgcgaccgc gcttgcgagc gaactgatcg ccgctctacg catgaccgac cacttcgagc
     2941 ggccgcggcg caacaaggcg ctgaccgtgg ccgccgagat gcagtgggcg ctgctgccgc
     3001 ccaccgcgta ccacgacgat cgggtcagtc tggccggttt ggtcgagccc gcgtactccg
     3061 ttgccggcga cgccttcgat tggagcgtcg cgcgagaaac ggtcaccatc gcggtcttcg
     3121 acggcgtcgc gaggggcgta acggcggcac ttgccactgc cctgtgcgtc aatgcgcttc
     3181 gtaacgcgcg gcgagcgccg gtgccacttg ccgaccaagt agcgctcgcc gaccaggctc
     3241 tgtacgcgca ctttggaggg aagagccaca tctcgacgct tgtcgttcaa ctcgatctgc
     3301 gcagcggctc agcggtggcg atcgacgcgg gttcacccca gctgctcgtc gttcgcgatc
     3361 gccaggtcaa caccgtcgtg ctggagccgc aacttccgct cggcatgttc gaagaaacgc
     3421 actatcgtga gcagcccctg acgttcgttc cgggcgaccg gctcatcatc gtgagcgacg
     3481 gagtgcatgc ggccggagag ccgatagcgt ttggcgttac tgcgcttcgc gaagccatcg
     3541 actccacggt cgaaagcagc ccgcccgaga cagttcgaca actcatcgct gcccttcacg
     3601 tgcaccacgg cgacgaactc gaggacgacg ccgtggtgct gtgcctcgac tgggacgcgt
     3661 gaccagtgcc tatgccttgc cgccagcggc cgcatattgc gccaggaatt cgtccccgag
     3721 ggccttgcgt tcggctgcgt tgcgccccga ctgaatcgtc gggaactctt ccttctcctc
     3781 agcctcggcg tggtcagaaa cagcctgctt gaactcggcg aactgcgcgt cgaacgtggc
     3841 gccgtcgacg tcgaggtctg acaacgcagc gatcaagtcg tccgcttcgc tctcctcggc
     3901 attgcgcgcg tcgacgacat cttctcccgc agtctccttc gtaactggtc gaacgacgga
     3961 ctcctccgcg gtctcatgtg ccttcagtgt ggtcgtcagc ttggtgaaga gcttcttgcg
     4021 ctcggcaccg gccgccgcct cgatctgatc cagcatgtcc ttcacttcgg cgtgctgagt
     4081 cagaaggatc cggacgatat cgccttcggg gagcttcgcg gccttggcgc ggtcctcttg
     4141 tgcgtcaccc atggtgagtc tcctcggtag tagttgtgac ttctcggatt acgagttctt
     4201 cttcttgccg atcgaaggga accgcttctt gaccgccgct cgcactgtct tacgttcggc
     4261 ggggctcccc ttctgcgcga cgcgtgcgag tgcgttgcgt gcatgtgcct tgtcggggat
     4321 cgggtacttc cgtttgccag gtagcgcgaa cgacttgctg ctcaacttct tacgctcctt
     4381 ggtgctactc aaatccttcg ccacggttct caaccccttc tggtgcgggt cccgaatagg
     4441 gacgtcgata cccggagtag gcgtgtcgaa acaccccgaa ttggcaggtg agaaagggtc
     4501 ttctggtggg tacacgggtc gccatctgtc caccgagtac ccgccacctt gtcggcaaac
     4561 ggccaggcgt cgccgccgaa cggcagtcgc tggaggacgt cgcggcaccg ctgtcagcgg
     4621 ctcgccgcat tcccgagttc ggaagcgggt acggggcacc tcgacggcgc cgaggtcacc
     4681 cgcgttcgat cgatgttccc agcagcccga ggggtggcgc cccgaggtcg agcatcgcgg
     4741 cgtggaaccg gctgtgcgag tagcccgtgc cccacgcgtc gcgtgccttc gactgcgttt
     4801 cgcggatgat catttttccc caggtatagc ggccatacgt cgggtcgaac gtcgcgcggg
     4861 cggcttcggc tctagctgcg cgacccgatg cgaacgcgtc cgactcgaac ctgcgcgccg
     4921 cgtcgtccac tgtcattgcg cctgtgtgca cgccgatcgc tgccgcgagc cgagtcaccc
     4981 gcatcagcgc ttcgatcgca atgccggcga cgaatcgcgg atcaccggac cggaagccga
     5041 ggtcgagaca caactcttcg gtgtagtgcg cccagccctc gacgaacgcc ggcgacatca
     5101 gaatgcggcg caccggtgtc ggggctcggc gcatcgcccg gctgtgcgcg aagtggccgg
     5161 gcgacacctc gtgcagcgtg atgcctggca aggtggcgtg gctgaagact tggagccatt
     5221 cctcgatctc attcggcggc cacgtcggtt ctggcggagt gacccagtag aacgagggtg
     5281 cgtcagcctc ttcgggtgca gcccacgcca tcatggcggt ggcccagcgc cgcgattcgg
     5341 gtgcagttgc cacgagacac gcgccgtcgg tgtagggcac cagatcgtgt tcgcgggtga
     5401 acgcgagaac ctcgtcgacc tgcgcctgcg cctcgctgag cacgccgtcg gcgtctgggt
     5461 ggtcggccat gagcttggcc acgacgtcag gcagttcggc gtcggggtcg atgtgcttgc
     5521 acgcgtcgcg caggattgcc gtctgccgat cgcgttcggt atcggcgagt tcggccaacc
     5581 gctcgacgtc gaccgtcgtc gcctcgaccg tggccatcaa tgcagcgaga ccagcggcgc
     5641 ccagtgcagc ctcgggtctg ccgttctcgg cggcgtcatg caagtgcgac atcagccgcg
     5701 cgtgtgcagc caatgccgcc ggggcgtgcg gatcggccgc atcgatggcg gcggcgaggc
     5761 cgttgaatgc gccgagcaac gacgtcgcga ccggcgccgc aacggcgtcc agcgtttcga
     5821 tcgtcatgtc gatggcgtcc ggccatgctg cgacgtgcgc actcttcgcg gcagctcgtt
     5881 gttcggccgg cgcgtactca cggtcgtaac acgacacgtc gaggttggcc aagtgcagta
     5941 cagggttgct gcggtgcagt tcgagcttgc cgaaaatgag gcgggcgttg tcttcgaaag
     6001 cacgcagatg ggcttcgtcg tgggtgtcat tgagcggcgc tccgccgagc ttcgcaagat
     6061 tggcccggac gccgtggggt gagaggtctt gtatcagacc gtcatattcg tgcaggccag
     6121 ccgcttcccg cacatcgccc atgaagaggt cgcacaccgc ccggagtcga tggtccatag
     6181 acccacgtta tccccgcctc gtgggcgcat accgccgctc gaccgcaatg tcgcgccgga
     6241 aagtcgcccg atagagtcgg ccgacaggga ggtcgggcga tggcgggtgg ccttgcgttg
     6301 aggatcgtcg tatgcgtcaa gcaggtaccc gacacgtggt cggagaagcg cctcgacccg
     6361 gctgaccaga cgctcgaccg cgcggctgct gacggtgtca tcgacgagct cgacacctac
     6421 gctgttgaag aggcactgcg catcaaggag tcgaacgagg gcagcacggt cgtcgtcctg
     6481 acgatgggcc ccgccggcgc tgccgacacg ctgcgcaaag cgctgtcgat gggcgccgac
     6541 gaagcagtgc acgtcgtcga cgacgcgttg cacggatctg acgcgctcgc cacgtcgctg
     6601 gttctcgcga aggcgatcga acgcaccggc ttcgacctcg tcgtgctcgg ttccgaatcg
     6661 accgacgctc gcatgggtgt tgtgccggcg atgcttgccg aacgactcgg tgtgccgcaa
     6721 gcgagcttgg cgagcaaggt cgaggtcgag tcaggcaccg ttcgcgtgca tcggcagacc
     6781 gagaccggtg tggccgtcgt ctcggcagat ttgccggctg tggtgagcgt gctcgagaag
     6841 atcaacgagc cgagataccc gtccttcaaa ggcatcatgg cggcgaagac aaagccgacg
     6901 gcgacgtggt cacttgccga cctcggtatc gacgcgtctg ctgtcgggct ggcggctgct
     6961 gcgacgcagg tgatcgatgc gacccagcgg ccaccgcgtg gtgatcgcac gatcatcaag
     7021 gacgacggcg atgctggcgc acgcatcgcc gatttccttg tgtctcgcaa gcttctctaa
     7081 ggacggaaga gcgatggcgg acgtactcgt actggtcgac gacgtcggtg tcggcaagcc
     7141 gacgcttgag atgctgacgc ttgctgcgcg cctcggatca ccggtcgctg tgttgctcag
     7201 cggtggcgcc gacgtcgaga agctcgcgca gtacggtgcg accgcggtgc tcgtcgcatc
     7261 ggacgatcag ctcacctcgg tcgtcgtcgc gccggccgtt tcgttgctcg aatcactggt
     7321 cgcgacgcgg tcgcctcgtg cggtcttgat cgcgtcgacg gcggaaggca aggagattgc
     7381 cggccgcctc ggcgtccgcc ttgatgctgg ggttctgacc gacgctgtcg acgttgcgct
     7441 cgatggcgat gccgtcgtgg tcgaacaatc agtgttcggc gggtcgaccc ttgtgcactc
     7501 gcgggtgact cgaggcgtgc caatcatcac ggtgcgaccc aacagcatca cggcagtggt
     7561 ggcgcaggtg actgcggatg tgtcggtcgt ctcggttccg gcgttggctg ggcggcacgc
     7621 ggtcgttgcc tcgcgctcta ccgaatcggc gggcggtcga cccggactgt cagaggcgac
     7681 cgtcgtcgtt gccggcggtc gcggagtcgg tgccgccgac aagttcgccc tcatcgagga
     7741 gcttgccgat gccatgtcag cagcggttgg cgcgtcacgc gccgcgaccg acgcggggtg
     7801 gtatccccac gccgcgcagg tcgggcagac cggagtcacg gtcgcgccgc agctctacat
     7861 cgcctgcggc atctcgggcg cgattcagca tcgtgcaggt atgcagacgt cgaagacgat
     7921 cgttgcgatc aacaaggacg ccgaggcgcc gatcttcgag ctcgccgact tcggcgtggt
     7981 gggcgatctg cacgtggtga tacctcagct gatcgccgag atcgctcgcc gcaagggcta
     8041 acctgcgacc gtcagacagt tacagggctc ttgacccgta ccgtctgaca gtcgatgacg
     8101 tggccttagt cgacgacgcg tagggccact ttgcggttgc cgccgtgctg cttgactaac
     8161 gcgaaccaca gcggcaggag cgcgtcgacc gtggccactg attcggcgtc gccgacgtac
     8221 gcgggtcgca agccgaccgc cgcgatgagc acctcagccg tctcgcgagc cgcgggatcc
     8281 gccgcgaaga agaggtcggc gcccggcagc ggtgaggcga agttctccca gccgagtgtg
     8341 ttgaacgccc gcacgtagtg agcgcccggc gcagccgccc cgatctccgc acggctgttg
     8401 acggcagcag cgcctatgtg gttggtcgcg tcgacaacga cctttccgtc gagcgcagcc
     8461 ccgagctccg atgcaacctc gccgacggcg ccgcccggca ctgcgagcac cacgacttcc
     8521 gcaccggcaa tc
//
LOCUS       DASWVF010000220         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439358, whole genome shotgun sequence.
ACCESSION   DASWVF010000220 DASWVF010000000
VERSION     DASWVF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439358"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGD55_04050"
     CDS             complement(<1..189)
                     /locus_tag="VGD55_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869547.1"
                     /translation="MRARSPRTGEEDAPSDFRTIEAPLKRAFEIGQAHLVPVEVVSYT
                     REVMAATAAKPQAMTVAFL"
     gene            complement(218..1378)
                     /locus_tag="VGD55_04055"
     CDS             complement(218..1378)
                     /locus_tag="VGD55_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869548.1"
                     /translation="MDRPQGYRLSADAHGGTVAMGGGAGERAPGSEGHDTGNIVAGLR
                     GLHRLRRCLNGLGDLPAFVLRHDVRSGRIDWWELLRDDRHLRPYDFEFRFSVAGRRDA
                     YHAANIAALRSSGSAQVSTLLGFSDLYVPLVIDRDFTLIFFCGQWATDVPTRDTITGA
                     WRDLSGQEPVFADALFRTWARCCLRVPVLNAALRDGLIAMGQLLGRLFSDDSPAAERA
                     VDSLREQVFLPFIHDDSWVESCLDTTGLTRPPWGLDEFLDVRIVEETQLRHRPSQLAV
                     VVPNLTTRGGRDGLDIWIQQRQFQHQALLVAAQAPDMVAASLGDQGILVALAARPSLR
                     GARTPGVLRGAVPRATTDLARADWFQGGRRIGHGGRARAGSRAVLPLRRGGC"
BASE COUNT          278 a          513 c          513 g          236 t
ORIGIN      
        1 caggaatgcg acagtcatcg cctgcggctt cgcggccgtc gccgccatga cttcgcgcgt
       61 gtacgagacc acctcgacgg gaacgagatg cgcctgaccg atctcgaacg cacgtttcag
      121 gggtgcctcg atcgtacgga aatcggaagg cgcgtcctct tctccggtcc gtggcgagcg
      181 ggcacgcacg agccggtcgc cgttccggag cgcggtgtca acagccgccg cggcgtagcg
      241 gaagcactgc ccgagaccct gcccgggcgc gacctccgtg cccaatccga cgaccgccct
      301 gaaaccagtc cgctcttgca aggtctgttg tagcgcgcgg caccgcccct cgaagtacgc
      361 cgggcgtgcg cgccccccga aggctcggcc gcgcggcgag cgcgaccagg atgccttgat
      421 cgcccaggct cgcggccacc atgtcgggtg cctgcgcggc aaccaacaat gcctggtgct
      481 gaaactggcg ctgttgaatc caaatatcca acccgtcgcg gcccccgcgc gtcgtcaggt
      541 tggggacgac gactgccagt tgggacggcc ggtgacgaag ctgcgtctcc tcgacgattc
      601 tgacgtccag aaattcatcc aggccccacg gcggccgggt gagacccgtg gtgtccaaac
      661 atgactcgac ccagctgtca tcgtggatga agggcaggaa gacctgctcc cgcaggctgt
      721 cgacggcgcg ctccgcggct ggcgagtcgt cggagaacag gcgcccgagg agctggccca
      781 tcgcgatcag gccatcgcgg agtgcggcgt tgaggaccgg cacacgcaag cagcatcgcg
      841 cccaggtgcg aaagagagcg tctgcgaaca ccggctcctg gccggaaagg tcgcgccagg
      901 caccggtaat cgtgtcgcgc gtcgggacgt ccgtcgccca ttggccgcag aagaagatga
      961 gcgtgaagtc gcggtcgatg accagcggaa cgtagaggtc actgaagccg agcagagtgg
     1021 agacctgcgc ggagccgctg ctcctgaggg cagcgatgtt cgccgcgtga taagcgtcgc
     1081 gtcgaccagc cacgctgaaa cggaattcga agtcgtaggg acgcagatgt cggtcgtctc
     1141 gcagcagctc ccaccagtcg attcgaccgg accggacgtc gtgacgcagg acgaacgccg
     1201 gcaggtcgcc cagaccgttc aagcaccggc gcagccgatg caggccacgg agcccagcaa
     1261 cgatgttgcc ggtgtcgtgg ccttcgctac ccggcgcccg ttccccggcg ccgccaccca
     1321 tggcgacagt gccgccgtgg gcgtcggcgc tgagtcggta cccctggggg cggtccactc
     1381 ctgttactgc caagagcccg acgcatgttg aaaagtcaga tgaacgtcag gttcgaagca
     1441 cggtcggttt gtgcgcagcg cgtcgtgtct gggttgatcc tgcggctact gcaacgcgaa
     1501 cgcctagaat cgtctcggaa cagcacccta ccgttcttac
//
LOCUS       DASWVF010000221         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_311710, whole genome shotgun sequence.
ACCESSION   DASWVF010000221 DASWVF010000000
VERSION     DASWVF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311710"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGD55_04060"
     CDS             <1..369
                     /locus_tag="VGD55_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HEY0869549.1"
                     /translation="IGNMIRQLLEEVRSAPLDEAGRVRLKEIHAASVKELESGLSPEL
                     VDELERITLPFSESEIPSESELRIAQAQLVGWLEGLFHGIQTALVAQQMAAHAQLEQM
                     RRALPPGATPDGQRAPGMYL"
     gene            complement(370..1176)
                     /locus_tag="VGD55_04065"
     CDS             complement(370..1176)
                     /locus_tag="VGD55_04065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0869550.1"
                     /translation="MPQLSPPRLVATDLDGTIIRSDGTISTRTVKALAAAEKAGATVV
                     LVTGRPPRWIEGIAEQTDHHGIVICSNGAIVYDLPTEKILRSTLIPSSLVMSVVARLS
                     TALPDLGFAVENGFDVSRSHTYRGGWVLGADLGLPTYDLDVVIAQDAAKLLASHPEMD
                     ADELLVVARGLVGDLVEPTHSNGRGLVEMGPIGVTKGSALAAMAGELGIDRADVVAFG
                     DMPNDLPMLAWAGTSYAVANAHPDVLAAVELKTLSNNDDGVALVLEELFG"
     gene            complement(1199..2467)
                     /gene="serS"
                     /locus_tag="VGD55_04070"
     CDS             complement(1199..2467)
                     /gene="serS"
                     /locus_tag="VGD55_04070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0869551.1"
                     /translation="MIDLKLVRDDPDRVRASQLARGEDPALVDALLAADEQRRAAVLR
                     FDTVRGEQKTLGKAVASAKSDADRQPLLARAKDLAAEVREAEAEQSTADATLRDAMLV
                     LSNVIEPGVPEGGEDDFALVAEVGTPPSFSFPPRDHVELGAILGAIDVERAAKVSGSR
                     FYYLTGVGALLELALVNMAMKQAVDAGFTPMLPPALVRSRAMEGTGFLGQAAENVFRL
                     EADDYYLVGTAEVPLAAYHMDETLPADDLPLRYVGFSPCFRREAGTYGKDTKGIIRVH
                     QFDKVEMFTFCPPEDAAAEHQRLLGWERQFLEALEVPYRVIDVASGDLGSSAARKYDC
                     EAWVPTQGKYREVTSTSNCTEFQARRLNIRMRDREGVRSVATLNGTLVAIPRTIVAIL
                     ENHQRADGSVVVPAALRPFLGRDVLEPVNR"
     gene            complement(2477..3418)
                     /gene="pheA"
                     /locus_tag="VGD55_04075"
     CDS             complement(2477..3418)
                     /gene="pheA"
                     /locus_tag="VGD55_04075"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY0869552.1"
                     /translation="MSARYAYLGPEGTFTEAALRTIPGAEAALLLPFRSVSAALDAVR
                     RGEVDAALVPLENSVGGGVASTIDELSTGELLHISREVLLDVGFALAVRRGSPRSSLK
                     RVAAHPQAEAQCRRWIADNLSDVEIVTSASNAEAASAVASASSGLDAAITSALAAQRY
                     GLEIVEDDIHDHAGAVTRFVLVELPSAPKVPTGTDKTSLVAFIGEDHPGALLEVLTEF
                     AVRGINLTWIESRPTGGGLGRYCFSIDCEGHIVDARVGEALMGLRRICTDVRFLGSYP
                     RADGLQPTMRRGTSDSDFHDAAAWLDDVRSGRTDALG"
BASE COUNT          612 a         1198 c         1170 g          559 t
ORIGIN      
        1 atcggcaaca tgatccgcca gctgctcgaa gaggttcggt cggcgccgct cgacgaggcc
       61 ggccgggtcc ggctcaagga gatccacgcg gcgtccgtca aagagctcga aagcggtctg
      121 tcaccagagc tggtcgacga actcgagcgc atcacgttgc cgttcagcga gtccgagatc
      181 ccgagcgagt cggagttgcg aatcgcgcaa gcacagcttg tcggctggct cgaaggtctc
      241 ttccacggga ttcagaccgc gctggtcgcc cagcagatgg cagcccatgc ccaactcgaa
      301 cagatgcgcc gcgcgttacc tccgggcgct actcctgacg gccagcgcgc gccgggcatg
      361 tacttgtagc tagccgaaca gttcctcgag caccagcgcc acgccgtcgt cgttattact
      421 caacgttttc aactcgaccg ccgcgagcac gtcggggtgc gcgttcgcga cggcatacga
      481 cgtacccgcc caagcgagca tcggcaggtc gttaggcatg tcgccgaacg cgacgacgtc
      541 ggcgcggtcg atgccaagtt cgccggccat cgccgccagc gcgctgcctt tcgtgacccc
      601 gatcggaccc atctcgacca ggccgcggcc attggaatgc gtcggctcga ccagatcacc
      661 gacgagccct cgagccacga cgagcaactc atcggcgtcc atctcaggat gtgacgcaag
      721 cagtttcgcg gcgtcttgag cgatcaccac gtcgaggtcg taggtcggca agccgaggtc
      781 ggcgccgagg acccagccgc ctctgtaagt atgcgagcgg gagacgtcga agccgttctc
      841 gacagcgaat cctagatcag gcagcgcggt cgaaagccgt gcgacgacgc tcatcaccag
      901 cgacgacggg atcaatgtcg agcgcagaat cttctcggtc ggcaggtcat agacgatcgc
      961 gccgttcgag cagatcacga tgccatggtg atcggtttgc tcggcgatcc cctcgatcca
     1021 tcgcggcggt cgaccggtca ccaaaaccac ggtcgcgccg gccttttcgg cagcggcaag
     1081 cgccttaacc gtgcgggtcg aaatggtgcc gtcagacctg atgatcgtgc cgtcgagatc
     1141 ggtggcgacc agacgtggcg gtgagagttg tggcatgtcg atcaaccgta tccctgagtc
     1201 agcggttgac tggctcgagt acgtcgcggc cgaggaacgg gcgcagcgcg gcgggcacga
     1261 cgaccgagcc gtcggcccgt tgatggttct ccaggatcgc cacgatcgtg cgcggaatcg
     1321 ccaccagcgt gccgttcagg gtggcgaccg agcgcacccc ctcgcggtcg cgcatgcgga
     1381 tgttcagtcg ccgcgcctgg aactcggtgc agttcgaggt cgacgtcacc tcgcggtact
     1441 tgccctgcgt cggaacccac gcctcgcagt cgtacttgcg cgcggcgctc gagccgagat
     1501 cgcccgacgc aacgtcgatc acccggtagg gcacctcgag tgcctcgagg aactggcgct
     1561 cccaaccgag caaccgctga tgctcggcgg cggcatcctc cggtggacag aacgtaaaca
     1621 tctcgacctt gtcgaactgg tgaacgcgga tgatgccttt cgtgtccttg ccgtaagtgc
     1681 cggcctcgcg ccgaaagcac ggtgagaatc cgacgtatcg caacggcagg tcgtcggccg
     1741 gcagcgtctc gtccatgtgg tacgccgcga gcggcacctc cgccgtcccg acgaggtagt
     1801 agtcgtcggc ttcgagacgg aacacgtttt ccgcggcctg gccgagaaag ccggtgccct
     1861 ccatcgcccg cgaccgcacg agggccggcg gcaacatcgg cgtgaacccg gcgtcgacgg
     1921 cctgcttcat tgccatgttc acgagcgcga gctcgagtaa cgcgccgacc ccggtgaggt
     1981 agtagaagcg cgatcccgaa accttggcgg cgcgttcgac gtcgatcgcg ccaagaatcg
     2041 ctccgagctc gacgtggtcg cgcggcggga acgagaacga cggcggcgtg ccgacctcgg
     2101 caaccagcgc gaagtcgtcc tcgccgccct cggggacgcc cggctcgatg acgttcgaga
     2161 gcacgagcat cgcgtcgcgc agtgtcgcgt cggcggtgct ctgctcggcc tcggcctcgc
     2221 gtacctcggc ggcgagatcc ttggcccgag caagcaacgg ctgccggtcg gcgtccgact
     2281 tggcgcttgc gaccgccttg ccgagcgtct tctgctcacc gcgcacggtg tcgaagcgga
     2341 gcaccgccgc acgccgttgt tcatcggcgg cgagaagagc gtcgacgagt gccgggtctt
     2401 cgccccgtgc gagttgtgaa gcgcgaacac ggtcgggatc gtcgcgcacg agcttgaggt
     2461 cgatcacccg ccgaggctac ccgagcgcgt cggtgcggcc ggagcgaaca tcgtcaagcc
     2521 aggccgcggc gtcgtgaaag tcggagtcgg aggtgccccg ccgcatcgtc ggctgcagcc
     2581 cgtcggcacg tgggtagctg ccaaggaagc ggacgtcggt gcagatgcga cgcagcccca
     2641 tcagcgcctc accgacgcgg gcgtcgacga tgtgcccttc gcagtcgatc gagaagcagt
     2701 accgcccgag gccgccgcca gtcggacgcg attcgatcca ggtcaagttg atgccgcgca
     2761 ccgcgaactc ggtgagcacc tcgagcaggg cgcccgggtg gtcttcaccg atgaacgcca
     2821 ccagcgatgt cttgtcggtg ccggtcggca ccttcggtgc cgagggcagc tcgaccagca
     2881 cgaatcgggt gaccgcaccg gcatgatcgt ggatgtcgtc ttcgacgatc tcgagcccgt
     2941 accgctgggc ggccagcgcg gacgtgatcg ccgcgtcgag acccgacgac gcagaggcga
     3001 ccgctgacgc tgcttcggcg ttggaggcag acgtgacgat ctcgacatcg cttaggttgt
     3061 cggcgatcca acgcctgcac tgcgcctcgg cctgcgggtg cgctgccact cgcttcagcg
     3121 acgaacgagg cgaccctcta cgcacggcga gcgcgaaccc aacgtcgagc agcacctcgc
     3181 ggctgatgtg cagcaattcc ccggtcgaca gctcgtcgat cgtcgaggca acgccgccgc
     3241 ccactgagtt ctccaacggc accagcgctg cgtcgacctc gccccgccgc accgcgtcga
     3301 gcgccgccga cacgctgcgg aagggaagga gcagcgccgc ctcggctcca gggatcgtac
     3361 gtagcgcagc ctcggtgaaa gtgccttcgg ggcctaggta ggcgtagcgc gcagacatgc
     3421 ccgcacccta gcgcgagcgg ctcacgtctc gatcgggtcg acggcgtgcg ccgctatctc
     3481 gtcatcatcg aagagccgga cctcgtcagc cacaacgcgc ttgatctgca cggccggag
//
LOCUS       DASWVF010000222         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439441, whole genome shotgun sequence.
ACCESSION   DASWVF010000222 DASWVF010000000
VERSION     DASWVF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439441"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGD55_04080"
     CDS             complement(<1..384)
                     /locus_tag="VGD55_04080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869553.1"
                     /translation="MTDDWRAINLANWESRVAVHVGPGGYSLDTFDNPTHLSSVVRYD
                     LPRLGRLDGLDVVHLQCHLGTDTVSLARLGARSATGLDFSASALLRARQLATRAKADV
                     SFVDGDVYDAVELLGPHQFDLVYTGI"
     gene            645..>2255
                     /gene="groL"
                     /locus_tag="VGD55_04085"
     CDS             645..>2255
                     /gene="groL"
                     /locus_tag="VGD55_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460405.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0869554.1"
                     /translation="MSKIIAYDETARRALERGMNQLADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPMALKRGIEAAVASVSAALLARAKDVETREQIASTASISAADPAIGEMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISPYFATDTERMEAVLDDPYV
                     LVVNSKIASVKDLLPLLEKVMQSGKPLAIIAEDVEGEALATLVVNKIRGTFRSVAVKA
                     PGFGDRRKAMLGDISILTGGQVISEEVGLKLETAGLDLLGKARKVVVTKDETTIVEGG
                     GDAEQIAGRVNQIRAEIEKSDSDYDKEKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALLQAGRTAFEKLNLEGDEATGARIVEIALEAPLK
                     QIAINAGLEGGVVVEKVRNLEPGWGLNAATGEYVDLIKAGIIDPAKVTRSALQNAASI
                     AALFLTTEAVVADKPEKAGAAAGGGGMPG"
BASE COUNT          429 a          727 c          767 g          332 t
ORIGIN      
        1 gatgccggtg tagaccagat cgaactggtg tgggccgagc agctccaccg cgtcgtacac
       61 atcgccgtcg acgaagctca catccgcctt cgcgcgggtg gccagctgtc gggccctcaa
      121 cagggcggac gccgagaagt cgaggcccgt tgccgagcgc gcgccgagcc gggcgagcga
      181 aacggtgtcg gtgccgaggt ggcattgcag atggacgacg tcgaggccgt cgagccggcc
      241 cagccggggc aggtcgtagc gcacgacgct cgacaggtgg gtcggattgt cgaacgtgtc
      301 gaggctgtag ccgcctggac ccacgtgtac agccacccgc gactcccagt tcgcgaggtt
      361 gatcgcccgc cagtcgtcgg tcatgccgat caatgtaaag agcgccggtc cggcccttta
      421 tcgcggccat catcagggcc aacgttcacc cgtcgtcggt tgcactcggg gggtctgagt
      481 gccaaacatg agctagcact cttagcacct gagtgacaga ttaatggccg ggccttcgag
      541 accctgccgc gccggcggac atgacaccgg cgaggcgtgg tcggccgtcg cgggcgtcga
      601 gtccggccgc acgcaacgtt tcccgacccg ggaggaccat ccgcatgtcc aagatcatcg
      661 cgtatgacga gactgctcgt cgcgccctcg agcgcggtat gaaccagctc gccgacgccg
      721 tgaaagtgac gctcggcccg cgtggccgca acgtcgtgtt ggagaagaag tggggcgctc
      781 ccacaatcac caacgacggc gtgagcattg ccaaggaaat cgagctcgaa gacccgtacg
      841 agaagatcgg cgccgagctc gtcaaggaag tcgccaagaa gacggacgac gtcgccggtg
      901 acggcacgac gaccgccacc gtgcttgccc aggcgctcgt ccgcgaaggc ctgcgcaacg
      961 tcgcagccgg cgcgaacccg atggcgctca agcgcggcat cgaagctgct gtcgcgagtg
     1021 tgtcggcggc gctgctggcg cgggcgaagg acgtcgagac ccgcgagcag atcgcgtcga
     1081 cggcctcgat ctcggcagct gacccggcga tcggcgagat gatcgctgag gccatggaca
     1141 aggtcggcaa ggaaggcgtc atcaccgtcg aggagagcaa caccttcggt cttgagcttg
     1201 agctcaccga gggcatgcgc ttcgacaagg gttacatctc gccctacttc gccaccgaca
     1261 ccgagcgcat ggaagccgtt ctcgacgacc cgtacgtgct cgtcgtgaac agcaagatcg
     1321 cctcggtcaa ggacctcctg ccgttgctgg agaaggtcat gcagagcggc aagccgctcg
     1381 cgatcatcgc cgaggacgtc gagggcgagg ctctcgcaac gctcgtcgtc aacaagatcc
     1441 gcggcacctt ccgctcggtc gccgtcaagg ctcccggctt tggtgaccgt cgcaaggcga
     1501 tgctcggtga catctccatc ctgaccggtg gtcaggtcat cagcgaggaa gtcggcctga
     1561 agctcgagac tgctggtctc gacctgctgg gcaaggctcg caaggtcgtc gtcaccaagg
     1621 acgagacgac catcgtcgag ggtggcggtg acgccgagca gatcgctggt cgcgtcaacc
     1681 agatccgcgc cgagatcgag aagagcgact ccgactacga caaagagaag ctgcaggagc
     1741 ggttggccaa gctggccggc ggcgttgcag tcatcaaggc aggcgcggcc accgaggtcg
     1801 agctgaagga gcgcaagcac cgcatcgaag acgcggtccg caacgccaag gccgctgtcg
     1861 aagagggcat cgtggccggt ggtggcgtcg cgctgctgca ggccggtcgc accgcattcg
     1921 agaagctcaa cctcgaaggt gacgaggcca ctggcgctcg catcgtcgag atcgccctcg
     1981 aggcacccct caagcagatc gcgatcaacg ctggcctcga aggcggcgtc gtggtcgaga
     2041 aggtgcgcaa cctcgagccc ggctggggcc tgaacgcggc gaccggtgag tacgtcgacc
     2101 tgatcaaggc cggcatcatc gaccccgcca aggtcacccg ctcggcgctc cagaacgcgg
     2161 cgtcgatcgc ggcgctgttc ctcaccaccg aagcagttgt tgccgacaag cccgagaagg
     2221 ccggtgccgc cgcaggcggc ggtggcatgc cgggc
//
LOCUS       DASWVF010000223         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_158956, whole genome shotgun sequence.
ACCESSION   DASWVF010000223 DASWVF010000000
VERSION     DASWVF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158956"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VGD55_04090"
     CDS             <1..569
                     /locus_tag="VGD55_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0869555.1"
                     /translation="LVSNNVGNTMRVAVTATNADGVSAETTAPTAVIAATSATTTATT
                     ATTTTITTTATTTTTATTTPVAVVQTARFNAVLRANQEVSRPHRTSTLAAGHFSAKVS
                     GKTLTWTLTFAHLSGRPTVVTLNKGTRAATGAAFKSLCRQCYSPARGTLTLTASQLAS
                     LLAGKTYVNIHTLRNTQGEIRGQINRVS"
     gene            1139..>1541
                     /locus_tag="VGD55_04095"
     CDS             1139..>1541
                     /locus_tag="VGD55_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY0869556.1"
                     /translation="MKETVTVAQVATVSSVLPSRVQEALGELVGAAQEGLLALSVGVG
                     LGVLAELMEEEVDEVVGPKGRHDPARRAVRHGHEAGEVTLGGRRVPVERPRVRSVDGA
                     QEVALQTYAHFADRDPLTRLVLERMLAGVSTR"
BASE COUNT          282 a          497 c          509 g          253 t
ORIGIN      
        1 cgcttgtcag caataacgtt ggtaacacga tgcgcgtagc ggtcaccgcc acgaacgccg
       61 acggcgtcag cgcggaaacg acggctccga cagcggtcat cgcagcaaca tctgccacga
      121 ctacagctac gacagcgacg actactacaa taaccaccac agcgacaaca acgaccactg
      181 cgacaacgac gccggtggcg gtcgtgcaga cagccaggtt caacgcagtg cttcgggcta
      241 accaggaagt cagccgcccg cacagaacgt caaccctcgc cgcgggccat ttcagcgcga
      301 aggtcagcgg caagacgctc acctggacgc tcaccttcgc acacctcagc gggcgcccga
      361 ccgtcgtcac cctcaacaag ggcacacgcg cagcaaccgg agcagcattc aagtcgctct
      421 gccgccagtg ctactcaccc gcccgcggca cgctgaccct gaccgcatcc cagctcgcct
      481 cgctactggc cgggaagacg tacgtgaaca tccacacgct caggaacacg caaggcgaga
      541 tccgcggaca aatcaaccgc gtgagctaac cggataacgc cggtcgcgcg ctcgaactga
      601 acgcaaccca ggctcgtggt gatcccttcg ccgagtggcc cgcctcgtca aaggcgatcg
      661 gcggcagcga ccctggcgtg ctggcccgga gttcgcgcgt gcccgtttca cgcgtgctgg
      721 gcgcgacgcc gttcgcatgc tcatcgatag tcagccgcag ccggacactc gtcggcaact
      781 tttgtagaga catgtatcgt tcggctcgat catcgggggg tcgtttgaag ccgtgccgcg
      841 tcatctggct gtcgtcaggt gcttggaact gatgtgatga cacgggctgc gttgctctcc
      901 gttctcgctg aagcggtcca gccagacagc gcgcgggctg tcctcatcgt cggttttgag
      961 agtattttgc ggtatttcgt gcgtcaaaag gtgactgaga gggcgacgcg ctcatcgctg
     1021 ctgtcggcgg gagaggttgt cccgtagctc gtagaacttg acggcggtcc ctccgcctcg
     1081 tccgctcctg ttgggagcgt tcggggtgat ccgtccaaag atcgaaggag gaaccgccgt
     1141 gaaggagacc gtaacggtcg cgcaggtcgc gaccgtctcg agtgtgttgc cgtcccgggt
     1201 gcaggaggcg ctcggtgagc tcgtcggggc cgcgcaggaa ggcttgctcg cgctgagcgt
     1261 gggagtcggc ctgggggtgc tggccgagct gatggaggaa gaggtcgacg aggttgtcgg
     1321 cccgaagggc cgacacgatc ctgcccggag agcggtgcgg catgggcacg aggctggcga
     1381 ggtgactctc ggcggccgcc gcgtgccggt tgagcggcca cgggtgcgca gcgtcgacgg
     1441 ggcgcaagag gtcgcgctac agacctacgc gcacttcgct gaccgtgacc cgctgaccag
     1501 gctggtgctc gagcggatgc tcgccggcgt ctcgacgcgc c
//
LOCUS       DASWVF010000224         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439464, whole genome shotgun sequence.
ACCESSION   DASWVF010000224 DASWVF010000000
VERSION     DASWVF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439464"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..265
                     /locus_tag="VGD55_04100"
                     /pseudo
     CDS             35..265
                     /locus_tag="VGD55_04100"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
     gene            complement(1643..2902)
                     /locus_tag="VGD55_04105"
     CDS             complement(1643..2902)
                     /locus_tag="VGD55_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0869557.1"
                     /translation="MRIGLIAPPWLPVPPPKYGGTEAVVDRLARGLAERGHDVLLFCT
                     GDSTCPVPKAWVFETAKRAELGQVVVELRHVIGAYDAMIGCDVVHDHSVVGPMYSERF
                     PELLTVATAHGPFRGDLHDVYRAIGPGVPIIAISHHQASTAGPIPIAAVIHHGVEPHA
                     FPVGTGGGGYYCWLGRMASYKGPREAIQVARRAGVPLLLAGKIEHENEVEYFRDQVEP
                     LLGGDIEYVGEIGPEQRAAFLGDAVALINPITWSEPFGLSMVESLACGTPVVAFPRGS
                     APEIVEDGVTGLLCKNIDEMVARLPEVARLDRSACRKVVEGHFSAERMVDEHIALYTR
                     LLQARRDVSVLDQPGHRWPARPDESYDITDATDIDGSRGAVDASADSHGHAVAEGDLG
                     DGEFVDTSRQLRIEPDAAVRDPRIETQ"
     gene            3010..3975
                     /gene="ligD"
                     /locus_tag="VGD55_04110"
     CDS             3010..3975
                     /gene="ligD"
                     /locus_tag="VGD55_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0869558.1"
                     /translation="MTLPHVAPMLATAGTLPSQSHDRFATEMKWDGVRAVAYWDGAEL
                     RLLSRNDNDVTAGYPELLALGDRLGSKAAILDGEIVAYDSTGRPSFGTLQERMHVRGA
                     KVAQLARDVPVTYLLFDVMHFDGANTMGLPYATRHELLAGLDLNANHLLTPPSFDEEP
                     KIVLAASLAQGLEGVVVKATDSVYLPGRRSPAWIKVKHQRMQEVVIGGWKPGEGARAG
                     RIGSLLLGVNNDVGELGYIGHVGTGFSDRALADLASRLRGLERKTSPFADEVPRLHAR
                     DARWVTPKLVGEVTYGEYTRDNRLRHPSWRGLRPDKDPGEVRRES"
     gene            3986..>4398
                     /locus_tag="VGD55_04115"
     CDS             3986..>4398
                     /locus_tag="VGD55_04115"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEY0869559.1"
                     /translation="MPYELAQINIGRLVAPIDDAQIADFVALLDPINAIADAAPGFIW
                     RLQTDEGNATAVQAFQWDADGSAGVIVNMSVWTDVEHLAAFVFGDMHREVMRRRREWF
                     HRMSEAYTTCWWVRAGHRPTTAEAEDRIRNLRANGP"
BASE COUNT          764 a         1467 c         1445 g          722 t
ORIGIN      
        1 cctcgtcgtg cggaggcgtt acgtcggcag cggcatgcgc gacgatctca cgttgagcaa
       61 tcacaacagg tacgacgtaa gcgtgcacgt acgcgtcgac gtagtcggtg acttcgccga
      121 cgtgttcgcc gtgaaggaag ggcgcgccga cgagcatcac ccatcagttc agcacaaggc
      181 gaacaccgac accttggtgc tgaccgaaac cgaacgcacg gtgcgcacga cgaccgtgca
      241 cttcggccac tcgccgaggg tcgacgatct cggcgccgcg tgggacgtca cgatccccgc
      301 cggcggcacc tggtcgacgt gctacgacgt gtcgatgcgg ctcggcgagc atgacgtgaa
      361 ggcccgctac aaatgcgggc agccggtcgg tgagtccgcg gcgtaccacc gcatcgaggc
      421 gtggcgcagc agcgtcccga ttctcgacac cgacgacacg gcgctcgagc tcgccgtcaa
      481 acgctcactt gaggatctcg gctcgttgcg actcttcgac cctgacgatg caacgcgggt
      541 cacgatcgcc gcgggcgcgc catggttcat ggcggtgttc gggcgcgact cgctgctgac
      601 gtcctacatg acgttgatcg ccgatccgga cctcgcactc ggagtgcttc agacgctcgc
      661 gcgatttcag ggcaccaagg tcgtcgccga cacggaggag caaccagggc gcatcttgca
      721 cgagatgcgt ttcggcagtg ccacgacgga ttcccttgcc agcgcggaca tctactacgg
      781 aacggccgat gcgacgccgc tgttcgttgt cctgctcggc gaacttcgcc gctggggtct
      841 cgcacgcgac gcggtcgatg agttgctgcc acacgccgac cgcgcactgg agtggatcga
      901 gcagtacggc gatcgggacg gcgatgggtt tgtcgaatat gaacgcgcca cgccgcacgg
      961 gctgatcaac caaggctgga aggattcatg ggacggcgta ccgttcgccg acggctcgca
     1021 gccgaagacc ccgatcgccg tcgccgaggt gcagggctat gtctacgcgg cttaccttgc
     1081 acgggcctac ttcgcgctcg aggccgatga ccagccgacg cacgttcgtt tccgcgacaa
     1141 agcggcggcg ttgaagcagc gcttcaacga agcgttctgg atgccagaag ccggttactt
     1201 cgcgatcggt ctcgacggcg acaaacggca aatcgacggc cgcggctcca acatcggtca
     1261 ttgcctgtgg agtggcattg tcgatgtcga caaggccgag tcggtcgcgc ggcacctcct
     1321 gtcgccggag atgttcagcg ggtggggcgt tcgcactctc gcgacgtcca tggcgacata
     1381 tgacccgatg agctaccact gcggttcggt ctggccgcac gacaacgcac tgatcgcttc
     1441 cggcctgatg cgatacggct tcgtcgacga ggcacaccgc gtcattcgcg gaatcctcga
     1501 tgcgtcagcc gcgtcgggcg gtcgcttacc cgagttgttc agcggcattt ccaggtcgga
     1561 cgtcgcggtg ccggtgtcct acccagcgtc gtgctcgccc caggcatggg ccgcagcgac
     1621 ccccctgcag ttccttcgac tgttactgcg tctcgatccg tggatccctc acggccgcat
     1681 ctggctcgat ccgcaactgc cggctggtgt cgacgaactc accgtcgcca agatccccct
     1741 cggcgacggc atgaccgtga ctgtccgcgc tggcgtcgac ggcgccccga cttccgtcga
     1801 tgtcagtggc atcggtgatg tcgtagctct catcaggtcg ggccggccac cggtgtccgg
     1861 gctggtcgag cactgagacg tcacggcgcg cctgcagcag tctcgtgtag agcgcgatgt
     1921 gctcgtcgac catgcgctcg gcggagaagt gcccctcgac gaccttgcgg cacgccgacc
     1981 gatcgagccg cgcgacttcc ggcagccgcg ccaccatctc gtcgatgttc ttgcacaaca
     2041 gcccggtgac accgtcttcg acgatctcag gcgctgaccc ccgtgggaac gcgacgacgg
     2101 gcgtgccaca cgcgagcgat tcgaccatcg aaagaccgaa cggctccgac caggtgatcg
     2161 ggttgatgag ggcaaccgca tcgccgagga acgctgcgcg ttgctcgggc ccgatctctc
     2221 cgacgtattc gatgtcgccg ccgagcaacg gctcgacctg gtcgcggaag tactcgacct
     2281 cgttctcgtg ctcgatcttg cccgctagca gcagcgggac gccggcccgt cgagcgacct
     2341 gaatcgcctc gcgcggtccc ttgtagctcg ccatccggcc gagccagcag tagtaccctc
     2401 cgccgccggt gccgaccgga aaggcgtgcg gctcgacccc gtggtggatc acggcagcga
     2461 tcggaatcgg gccggcggtc gatgcctgat ggtgtgagat cgcaatgatc gggacgccgg
     2521 gaccgatcgc ccggtacaca tcgtgcaggt cgccgcggaa gggcccatgc gcggttgcga
     2581 cggtgagcag ctcggggaag cgctcggagt acatcgggcc gacgaccgag tgatcgtgca
     2641 cgacatcgca accgatcatc gcgtcgtacg ccccgatgac atgccgcaac tcgacgacga
     2701 cctgaccaag ctcggcgcgc ttggccgttt cgaagaccca cgccttcggc accgggcagg
     2761 tgctgtcgcc cgtacagaag agcagcacgt cgtggcctcg ctccgcgagc ccgcgcgcga
     2821 gccgatcaac gacggcctcg gtgccgccgt acttcggcgg cggcaccggc agccacggtg
     2881 gtgctatcaa gccgatccgc atgtgacctc acgttcgaag cccgctcgac cggccacacc
     2941 gacgtgcccg gtgattggct cctacccgga aaagtcgcga tgatgcacag tcgtgagcag
     3001 gcccgcgaga tgacccttcc gcatgtagcg ccgatgctgg ccacggcggg aacgctcccg
     3061 tcgcagagtc acgaccgctt tgcgacagag atgaagtggg acggcgttcg ggcggtcgcg
     3121 tattgggacg gcgccgagtt gcggctcctg tcgcgcaacg acaacgacgt cacggcgggc
     3181 tacccggagc ttctggctct cggggatcgt ctcggctcga aggccgcgat cctcgacggc
     3241 gagatcgtcg cctacgacag caccggacgg ccgtcgttcg ggacgttgca ggagcgcatg
     3301 cacgtgcggg gcgcaaaggt ggcccagctc gcacgagacg tgccggtcac ctacctgctg
     3361 ttcgatgtca tgcacttcga cggtgccaac accatgggcc tgccctacgc gacgcgtcat
     3421 gagctgctag cggggctcga cctgaatgca aaccacctgc taacgccgcc gagcttcgac
     3481 gaggagccga agatcgtgct ggccgcgagc ctcgcgcagg ggctggaggg cgtggtggtc
     3541 aaggcaaccg acagcgtgta cctgccggga cgacggtcac cggcctggat caaggtcaaa
     3601 caccagcgga tgcaggaggt cgtcatcggc ggctggaagc ccggtgaagg tgcgcgggcc
     3661 gggcgcatcg gctccttgct tctcggcgtc aacaatgacg tcggcgagct cggctatatc
     3721 ggacatgtcg gaaccggctt cagcgatcgc gcgctggcgg atctcgccag ccgattgcgc
     3781 gggctcgaac gcaagaccag cccgttcgcc gacgaggttc cgcggctgca cgcgcgcgac
     3841 gcccggtggg taacgccgaa gctggtcggt gaagtcacct atggcgagta cacccgcgac
     3901 aaccggctac ggcacccgtc gtggcgcggc ctgcgacccg acaaagatcc tggcgaggtc
     3961 cgccgcgaaa gctagactcg cgcgcatgcc gtacgagctc gcacagatca acatcggtcg
     4021 gctcgtcgcg ccgatcgacg acgcgcagat cgccgacttc gtcgcccttc tcgaccccat
     4081 caatgcgatc gccgatgccg cgcccgggtt catctggcgc ctacaaaccg acgaggggaa
     4141 cgccaccgcc gtccaagcat ttcaatggga tgccgatggc agcgccggag tcatcgtcaa
     4201 catgtcggtc tggaccgatg tcgaacacct cgccgcattc gtgttcggcg atatgcaccg
     4261 cgaggtgatg cgccgtcgac gcgaatggtt ccatcgcatg agcgaggcgt acacgacgtg
     4321 ctggtgggtg cgagcgggtc atcggccgac cacggcggaa gccgaagatc ggattcgcaa
     4381 cctgcgcgcg aacggccc
//
LOCUS       DASWVF010000225         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_490159, whole genome shotgun sequence.
ACCESSION   DASWVF010000225 DASWVF010000000
VERSION     DASWVF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490159"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2085
                     /locus_tag="VGD55_04120"
     CDS             268..2085
                     /locus_tag="VGD55_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEY0869560.1"
                     /translation="MTGGRVREISSPAVVSIADDATLTDILRTHATVTPDAVLFRRRP
                     DNEGEEWTPVSAAEFFAQVEALAAGFLATGYRPGQRVGLLSRTRYEWTLTDYALWHAG
                     LVTVPIYETSAPDQIAWILGDSEAVGVVVETADHASTVSSIRDHIPDLRNVWVIDAGE
                     LDTLAASGRNAGPGALARARTSVNAASLASIIYTSGTTGRPKGCALTHRNMLYGATSA
                     VESLPALFVGNASALLFLPLAHVFARDIQVATMQAAVPVGHCPNTHSLQQDLEAFKPT
                     LLLAVPYVFEKLYSVAQQRAIDEGHGKIFAAASATAIRASEAIATGRVPLLLRMKRAA
                     FDVLVFRKLRGALGGEVKWVISGGAPLGTRLGHFFRGAGIPVLEGWGLTETTAASAVN
                     RPDAVKIGTVGPPLAGTTVRVDDDGELLVRGDHVFDRYWADPEATAAVRDDDGFFHTG
                     DLGDIDEAGFIRITGRKKEILVTAGGKNVSPAQLEDRLRGHPLIAQAMVVGDRRPYIA
                     ALITIDEDALTTWKTKVGKDSALTLDQLRDDPDLLAEIGAAIEEANRVVSRAESIRRF
                     RILDRDFTEAGGQLTPTLKLKRSAVLKEYADEIEALYAG"
     gene            2186..2893
                     /locus_tag="VGD55_04125"
     CDS             2186..2893
                     /locus_tag="VGD55_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869561.1"
                     /translation="MPAQVETSRLRVAFGPVVAVHSLDLAVQPGASVALVGRNGAGKS
                     TTLRALAGVLPATSGDVFVAGVDVSHDPATAKARIGYCPDVGGLIPRATPWEHLALAA
                     KLRGLRPDWTERARALLELFELAGAADRVTAGFSHGMGRRLSVVLAVLHEPQVLLLDE
                     PFDGVDPLGVDATLEVIAQAQAGGASVIVSTHLLELAAAACQEVVVLRAGRIVAAAPS
                     AELRGEEGQLRYRALLS"
     gene            2890..>3263
                     /locus_tag="VGD55_04130"
     CDS             2890..>3263
                     /locus_tag="VGD55_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869562.1"
                     /translation="MTTALPQAQLPALVRLRWQMVRAERVRIGLLVLAAFVPGLLCAA
                     VIAGQLLPRTRSFDVTVLAPTAFLGFAVLALVAPLASGGGNQLFPPEQLVAFPIRPST
                     HFLESILVAPLNLAWMSQFLLL"
BASE COUNT          508 a         1175 c         1066 g          514 t
ORIGIN      
        1 gctccggcgc tggccgcaga tcggtgaagc acaacgtgca ccacggcgag ccagcccgca
       61 ggagcgcact gcagtgcggg caacgcactt cgtcctgatc gctcacgccc gtgtcgtcgg
      121 caatcgggcc accgaccttg acccccgcgg atcaattggc ccaacgggcg ctactccatt
      181 tggcccaatg cgtgcaaaag ccgcccgctc cggatacgtt ccgtcgcggg gccggcccag
      241 gctggaacag tccgagtaac tgtcagggtg actggaggtc gggtgcgcga gatcagctcg
      301 cccgccgtcg tgagcatcgc cgacgacgcg acgttgaccg acattctccg aacgcacgcg
      361 acggtcacgc ccgatgccgt cctgttccgc cgtcgtccag acaacgaagg cgaagagtgg
      421 acgccggtca gtgccgccga attcttcgcg caggtcgagg cacttgctgc gggctttctc
      481 gcgacggggt accgacccgg ccaacgggtc ggcctgcttt cgcgcacccg ctatgagtgg
      541 acgctcaccg actacgcgct atggcatgcc ggcctcgtca ccgtgccgat ctacgagacg
      601 tcagcccccg accagatcgc ctggatcctc ggcgactccg aggcagtcgg ggtcgtcgtc
      661 gagacggccg accacgcctc cactgtcagc tcgatcaggg atcacatccc cgacctgcgc
      721 aacgtgtggg tgatcgacgc tggcgagctc gacaccctcg ccgcgagcgg acgtaacgcc
      781 ggtcccggcg cgctcgcgcg cgccagaacc tccgtcaacg cggcatcgct cgcctcgatc
      841 atctacacct cgggcacgac gggacgcccg aagggctgcg ccctcacgca tcgcaacatg
      901 ctctacggcg cgaccagcgc cgtcgaaagc ctgcctgcgc tgttcgtcgg caacgcgagc
      961 gcgctgctct tcctgccgct ggcgcacgtc ttcgcccgcg acatccaggt cgccaccatg
     1021 caagcggcgg tgccggtcgg gcactgcccc aacacccact ccctgcagca agatctcgag
     1081 gcgttcaagc cgacgctgct cctcgccgtc ccctacgtat tcgagaagct ctactccgtc
     1141 gcccaacagc gggcgatcga cgaggggcac gggaagatct tcgcggccgc ctccgcgaca
     1201 gcgatccgcg cgagcgaggc gattgcgacg ggccgagtgc cgctccttct gcggatgaag
     1261 cgggcggcgt tcgacgttct ggtgttccgc aagttgcgag gcgcgctcgg cggcgaggtc
     1321 aagtgggtga tctcaggcgg ggcgccgctg ggcacccggc tcggccactt cttccgcggg
     1381 gcgggaatcc cggtgctcga aggctggggc ctgacggaga cgacggccgc ctcggcggtc
     1441 aaccggcccg atgcggtcaa gataggcacg gtcgggccgc ccctggcggg caccacggtc
     1501 agggtcgacg acgacggcga gctactcgtg cgcggcgacc acgtcttcga caggtactgg
     1561 gccgaccccg aggcgacggc agctgtacgc gacgacgatg ggttctttca caccggcgat
     1621 ctcggcgaca tcgacgaagc gggcttcatc cgcatcaccg gccgcaagaa ggagatcctg
     1681 gtcacggccg gcggcaagaa tgtctcaccc gctcagctcg aagaccgtct ccgcggccac
     1741 cctctcatcg cgcaggcgat ggtcgtgggc gatcggcggc cctacattgc cgccctgatc
     1801 acgatcgacg aggacgcgct cacgacctgg aagaccaagg tcggcaagga ttccgcgctc
     1861 acactggatc agctccgcga cgaccccgac ctgctcgcgg agatcggcgc agccatagag
     1921 gaggccaatc gggtcgtgtc acgcgccgaa tcgatccgcc ggttccggat tctcgatcgc
     1981 gacttcaccg aagccggcgg tcagctgacc ccgaccctga aactaaagcg atcggctgtg
     2041 ctcaaggagt acgccgacga gatcgaagcc ctgtatgccg gatagcacca cggcgtcggt
     2101 tgccgcgtga caccctgacc caggggtttc acgttcggaa gggcacgcaa gcgcgggcac
     2161 cacacgcgca ccggggaggt gagcgatgcc cgcccaggtt gagacaagcc ggctgcgggt
     2221 cgccttcggg ccggtggtcg ccgtccattc actcgatctc gcggtacagc caggcgcttc
     2281 ggtcgcgctg gtcggccgca acggcgcagg gaaatcgacg acgctgcgcg cactcgcggg
     2341 ggtcctgcct gcgaccagcg gcgacgtctt cgtcgcgggc gtcgacgtca gccacgaccc
     2401 ggccacagcg aaagccagaa tcggatactg cccggacgtc ggggggctga tcccgcgcgc
     2461 aacgccgtgg gaacaccttg cgctggcggc aaaactgcgc ggtctgcggc ccgactggac
     2521 cgagcgcgca cgcgcattgc tcgaactctt cgaactcgcc ggcgcggccg atcgggtcac
     2581 cgccggcttc tcccacggta tgggccggcg gctgtccgtc gtccttgctg tcttgcacga
     2641 gccccaggtg ctgttgctcg acgaaccgtt cgacggcgtc gacccgcttg gggtcgacgc
     2701 gaccctcgag gtgatcgcgc aggctcaggc gggcggcgct tcggtcatcg tgtcgacgca
     2761 cctgctcgag ctcgcggccg cagcctgcca ggaggtcgtc gtgctccgcg ccggtcgcat
     2821 cgtggcggcc gcgccatcgg cagagctgcg cggcgaagaa gggcaactcc gctaccgagc
     2881 gctcctctcg tgaccacagc actcccgcag gcccagctac ccgcgctcgt caggttgcga
     2941 tggcagatgg tccgcgccga gcgcgtccgg atcgggctgc tggtcctagc cgccttcgtg
     3001 cctggcctgc tctgcgccgc cgtcatcgcc ggtcagctgc tgccccgcac ccggtccttc
     3061 gacgtgaccg tccttgcccc gacggcgttc ctcggattcg ccgtgctagc gctcgtcgcg
     3121 ccgctggcgt cgggcggtgg caaccaactc tttccgcctg agcaactcgt cgcgttcccg
     3181 atccgcccgt cgacccactt cctcgagtcg atcctcgtcg cgcccctgaa cttggcgtgg
     3241 atgagccagt tcctgctgct ttt
//
LOCUS       DASWVF010000226         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_337399, whole genome shotgun sequence.
ACCESSION   DASWVF010000226 DASWVF010000000
VERSION     DASWVF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337399"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGD55_04135"
     CDS             <1..492
                     /locus_tag="VGD55_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869563.1"
                     /translation="IDGVLADVRHRLHHLARRPKDWGAFFAAAKDDDVLEIGADFARQ
                     AAATHDIVYLTGRPAYLRAATQTWLDSQQLPAGTLLMRPEDDRRPSTVVKLHELRRLR
                     RESVVDLLVDDDPSVIDAARAAGFTVQLAEWMTALAAADPAQASLFDDDLLHNAQNRE
                     GRT"
     gene            complement(508..3417)
                     /gene="gcvP"
                     /locus_tag="VGD55_04140"
     CDS             complement(508..3417)
                     /gene="gcvP"
                     /locus_tag="VGD55_04140"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY0869564.1"
                     /translation="MNQSDPTSQHANKSLRSLETGRAFVDRHIGPDADEQAKMLAVIG
                     YGSLADLVARAIPTSIVDDRDRRSLIPPAASEVEVIAELRALAAMNTVATPMIGLGYF
                     GTHTPAVIRRNVLENPAWYTAYTPYQPEISQGRLEALLNFQTVISDLTGLPVAGASLL
                     DEPTAAAEAMALCRRNSSSSSHRFYVDADVLPQTQAVVQTRALPMGIEVVVADLNNGL
                     PDAECFGVLVQDPGTGGRVRDHAALAAKAHDAGALVVAATDLLALTLLRPPGEWGADI
                     AVGSSQRFGVPLWFGGPHAGFIAVRESLARSLPGRLVGQSIDADGKPAYRLALQTREQ
                     HIRREKATSNICTAQVLLAVAASMYAVYHGPQGLTDIARRTHRSASILAEGLRRSGIG
                     IVNDTYFDTVQVSVPGRAALVVAAALEQGINIRRVDAGTVGVSCDETTERRHLAGLWA
                     AFGTDLTAADIDAIDASIDDSYPEPVRRTSNFLRHKVFHQHRSETSMLRYLRSLSDKD
                     VALDRSMIPLGSCTMKLNATTEMEPISWPEFADMHPFAPTEQAAGYASLISQLESWLA
                     DVTGYDAVSVQPNAGSQGELAGLLAIRAFHAAQGSVERNVCLIPASAHGTNAASAAMA
                     GMRVVVVACDDDGNIDLDDLRAKLEAHADKLAAIMITYPSTHGVFESTVVDVCAAVHH
                     AGGQVYVDGANLNALVGLARPGRFGADVSHLNLHKTFCIPHGGGGPGVGPVAVRAHLA
                     PYLPNHPLSRVAGPATGPGPISGAPFGSAGVLPIPWAYIRLMGLEGLTRATEVAILSA
                     NYLARRLMPSYPVLYTGDGGLVAHECIIDLRPITKDSGVTVEDVAKRLIDYGFHAPTM
                     SFPVAGTLMIEPTESEGLDELDRFVEAMVSIRAEIAKVASGEWDRLDNPLHNAPHTAE
                     SMTAEWDHPYSRALAAYPVTALRRGKYWSPVSRIDGAYGDRNLVCSCPPPSAYET"
     regulatory      complement(3560..3653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3696..4319)
                     /locus_tag="VGD55_04145"
                     /pseudo
     CDS             complement(3696..4319)
                     /locus_tag="VGD55_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
BASE COUNT          748 a         1422 c         1456 g          699 t
ORIGIN      
        1 atcgatgggg tgctcgccga tgtccggcac cggctgcacc acctggcacg acggcccaaa
       61 gactggggcg ccttcttcgc tgcggcgaaa gacgatgacg tcctcgagat cggcgctgac
      121 ttcgcgcggc aagccgctgc gacccacgac atcgtctatc tgaccggacg accggcatac
      181 ctgcgcgcag cgacgcagac ctggcttgat tctcagcagc tgccggcggg aacgttgctg
      241 atgcggcccg aagacgatcg gcgcccgagc acggtcgtca agctccacga actgcgtcgc
      301 ctgcgtcgcg agtcggtcgt cgacctgctc gtcgatgacg atccgagcgt gatcgacgca
      361 gcacgcgcgg cgggcttcac tgttcagctc gccgagtgga tgacagcact cgccgcggct
      421 gacccagcgc aagcatcgtt gttcgacgac gacttgctgc acaacgcgca aaaccgcgag
      481 ggtcgcacct aaaggccctg cgtttgctca ggtttcgtac gccgacggcg gtgggcaact
      541 gcacacgagg ttgcgatcgc cgtacgcgcc gtcgatgcgc gagacaggcg accagtactt
      601 tccgcggcgc aatgccgtta ccggatatgc ggccaacgcg cgtgagtacg ggtggtccca
      661 ctcagcggtc atcgactcag cggtgtgcgg tgcgttgtgc aatggattgt cgagccggtc
      721 ccactccccc gacgcgacct tcgcgatctc ggcgcggatc gagaccatcg cctcgacgaa
      781 acgatcgagc tcatcgaggc cctccgactc ggtgggttcg atcatcaacg tgccggcgac
      841 cgggaacgac atcgtcggcg catggaaccc atagtcgata agccgcttgg cgacatcttc
      901 gaccgtgacg ccgctgtcct tggtgattgg ccgtagatcg atgatgcatt catgtgccac
      961 caagccaccg tcgccggtgt agagcaccgg gtacgacggc atgagccggc gcgcgaggta
     1021 attcgctgac aggatcgcca cctcggttgc tcttgtgagg ccctcgagcc ccatcaacct
     1081 gatgtaggcc cacgggatcg ggagcacgcc agctgacccg aagggcgcgc ccgagatcgg
     1141 ccctgggccg gtcgctggcc cagcgacgcg tgacagcgga tggttcggca ggtacggggc
     1201 gaggtgcgcc cgcacggcca ctggcccgac gccggggccg ccaccgccgt gcgggatgca
     1261 aaaagtcttg tgcaggttga gatgcgacac gtcagcaccg aaccggcccg gcctcgccag
     1321 cccgacgagg gcattgaggt tggcgccatc gacgtagacc tggccgcccg cgtggtgcac
     1381 ggccgcgcac acgtcgacaa ccgtcgactc aaacacgcca tgggtcgacg ggtaggtgat
     1441 catgatcgcc gcgagcttgt cggcgtgcgc ttcgagcttc gctcgaagat cgtctagatc
     1501 gatgttcccg tcgtcgtcgc aagcgacgac gaccacacgc atgccggcca tagccgcgct
     1561 cgccgcgttt gtgccatggg ccgaagccgg aatcaggcag acgtttcgtt cgacgctgcc
     1621 ttgggccgcg tggaaagcgc ggatcgccag cagtcccgcc agttcaccct gggatcccgc
     1681 gttcggctgt acggagaccg cgtcgtaccc ggtcacatcg gcgagccacg attcgagctg
     1741 gctgatgagg ctggcgtaac cagcagcctg ctcggtgggc gcgaacgggt gcatgtcggc
     1801 gaactcgggc cagctgatcg gttccatctc ggtggtcgcg ttcagcttca tggtgcaaga
     1861 cccgagcggg atcatcgagc ggtccaacgc gacgtccttg tcggacaagc tacgcaggta
     1921 ccgcagcatc gacgtctccg accggtgctg gtgaaacacc ttgtggcgga ggaaattcga
     1981 cgtccgtcga accggttccg ggtagctgtc atcgatgctt gcgtcgatcg cgtcgatgtc
     2041 agcagcagtg aggtcggtgc caaacgcagc ccacagccct gcgaggtgcc ggcgctcggt
     2101 tgtctcatcg caggagaccc cgacggtgcc ggcatcgaca cgcctgatgt tgatgccctg
     2161 ctcgagtgct gccgcgacga cgagtgcggc gcggcccggc acgctgacct gcacggtgtc
     2221 gaagtacgtg tcgttgacga tgccaatacc gctgcggcgc aagccttcgg ccaggattga
     2281 cgcgctgcga tgcgtgcgcc gcgcgatgtc ggtcaatccc tgcggcccgt gatagaccgc
     2341 gtacatcgag gccgccacgg caagaaggac ctgcgcggta cagatattgc tcgtcgcctt
     2401 ctcacgcctg atgtgctgct cgcgcgtctg caacgccagc cggtatgccg gcttgccgtc
     2461 ggcgtcgatc gattgaccga caaggcgccc cggcagcgac cgcgcgagcg attcgcgcac
     2521 ggcgatgaag cctgcgtgcg ggccaccgaa ccacaacgga acgccaaaac gctggctcga
     2581 gccgaccgcg atgtcggcac cccactcccc cggcgggcgc aacaacgtca acgccagcag
     2641 gtctgttgcc gccaccacga gtgcgccggc gtcgtgagct ttggcggcaa gcgcggcgtg
     2701 gtctcgcacc cgcccaccag taccggggtc ttgaacaagg acgccaaagc actcggcgtc
     2761 cggcagaccg ttgttgaggt cagcgacgac gacctcgatg cccatgggca acgcgcgggt
     2821 ctgcacaacc gcttgcgtct gtggcaggac atcggcatcg acgtagaaac ggtggctgga
     2881 cgacgaggag ttgcggcggc acagcgccat cgcctcggca gcggctgtgg gttcgtcgag
     2941 cagggacgcg cccgcgaccg gaagcccggt gagatcagaa atcaccgtct ggaaattgag
     3001 cagcgcctca agccgacctt gagaaatctc gggctggtaa ggcgtgtacg cggtgtacca
     3061 ggccgggttc tccaggacgt tgcggcgaat caccgccggg gtgtgcgtgc cgaagtagcc
     3121 gagcccgatc atcggagtcg ccaccgtgtt catcgccgcg agcgcgcgca gttctgcgat
     3181 gacctcgacc tcgctggcag cgggcgggat cagcgaccgg cgatcgcgat cgtcgacgat
     3241 cgaggtagga atagcgcggg ccaccagatc ggccagtgag ccgtacccga tgaccgcgag
     3301 catcttcgcc tgctcgtcgg cgtccggccc gatatggcgg tcgacgaagg cacggccggt
     3361 ttcgagggag cgcagcgact tgttcgcgtg ctggcttgtc gggtcggact ggttcacagg
     3421 ggcctccgga cgcaagggtc ggctttcccc ctctgtcgcg ggcacctgag agcttcaccg
     3481 acccgcggca ctgagatgcc ttccgggccg actttcaccg tcggtgacgt gccttgcggc
     3541 acgttctttc cagagttgcc tcgcctgctc ggtcgctggg cctgagagat tccggggggt
     3601 tgctccttcg gcgccccacg ctggcggcgt gagaactctc ccgtgcaggg ttgtcggcgt
     3661 ctccaacgct agcagcgcgc ggagccttag gcgcgctagg agccctcgga tgcaagccgg
     3721 atgccgaagc cgatcagcac cagcccggtg gcgcggtcaa ggcggcggcg tatcgcgggt
     3781 tggctcatcc atcgcgtgat gcggccggcg agcatcagga gcagcgcgaa ataggccgcc
     3841 gtctcgatga cgaagatcgc gccgaacacc agcgacgtgc tgccgaccgg atggcccgat
     3901 ggcacgaagc cgggcaggaa cgtcacgaag aacacgccga ccttcgggtt gagcaggtcg
     3961 gtggccaacc cggcgcggta gcccaccccg agcagtttgg gtggcgcggt tggcgtcatc
     4021 tcgtcggtgt gtttggcggt ccgggcccgt agtgagttca cgccgagcca cacgagttag
     4081 accgccccga ccagccgcag cgcggtgtat ccatcgcggc tggcgcgcag cagcgccgcc
     4141 aggccgagcg cagccgtgat aacccagacc gccaagcccg agagcacgcc cagaaccgtg
     4201 agcgtcgccc cgcgccgccc gtcgcggatg aggttgcgaa ccaccacgag ggtgtctggg
     4261 ccagggagca acacgatcaa tgccgccgcg aggctgaacg tgaccaccgc cgtcaacatg
     4321 actgg
//
LOCUS       DASWVF010000227         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_210154, whole genome shotgun sequence.
ACCESSION   DASWVF010000227 DASWVF010000000
VERSION     DASWVF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210154"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..514)
                     /locus_tag="VGD55_04150"
     CDS             complement(266..514)
                     /locus_tag="VGD55_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY0869565.1"
                     /translation="MAAPPLQGGSSGGPPTGSASGIRVAATTPTAQVLGELGVTVGQG
                     LPAAEVARRQARYGPNSVSSHRARLFPVLWHQLRQASG"
     gene            complement(565..993)
                     /locus_tag="VGD55_04155"
     CDS             complement(565..993)
                     /locus_tag="VGD55_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0869566.1"
                     /translation="MSTDTEVTRPLIVVGVDGSAESAKATAWAVEQARATGGTLDLVT
                     VWARPMSYGLPLVVGGYDPEREAQEVVEKIAADIDLPAPHLRKSVIHGAAPEVLVARS
                     GDADLVVVGSRGHGGFAELLLGSTSDHVVHHANCPVVVVR"
     gene            complement(1068..1697)
                     /locus_tag="VGD55_04160"
     CDS             complement(1068..1697)
                     /locus_tag="VGD55_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869567.1"
                     /translation="MAQVIWETAPDAHDYPAASSYLSLLVADDGLRDQLVAQLQAAPV
                     SHYKAKDLLRASQLALLTADNVHVAADLRKIRKSRPLSPVLLVRGDLLRGFPLQIADG
                     YHRICASYFVNENTDIPCRMIDLPNVVRAAATKAVAPAKKTAPAKKTAPAKKAAPAKK
                     AAPAKKAAAAKKVAKKSAPAKKAAPAKNVGVAVGAPPEVAPEAGSTTAR"
BASE COUNT          291 a          628 c          614 g          344 t
ORIGIN      
        1 gtgtcagacg tggtcgacgc ggtacttgcc gagtggaccg ctccggctga cagagcgaac
       61 gcgagcacgc cgacgccagc cactgaactc gtggcgatcg ccgcgctcac agccttcact
      121 cgccggtgtc cgcgagtgcg atgttccgat gtcatgttcg tcatgcaatg agcgtcgccg
      181 ccgctggtca cgcccggctg gacgctacct gaacgttgcc cgagcctttg actggccgaa
      241 cgagctacga ggttggtcac ctcgatcagc cggacgcctg tcgcagctgg tgccagagca
      301 cgggaaacag ccgggcgcgg tgcgacgaca cagagttcgg cccgtagcgc gcttgccgtc
      361 gggctacctc ggcggcgggc aggccctgac caaccgtgac gccgagctcc cccaggacct
      421 gggccgtggg tgtcgttgcc gcgaccctga tcccgctcgc ggaaccggtc ggcggaccgc
      481 ccgacgaacc gccctggagc ggcggggcgg ccaccgtcac agccggcgcc cgccgctcat
      541 ggtcgacccg ccgctaccta tcgactatcg gacgaccaca accgggcagt tggcgtgatg
      601 gacgacgtga tcgctggtcg acccaagcaa cagctcagcg aacccgccgt gcccgcgcga
      661 gccgacgacg acgaggtctg catcgccaga acgagcgacc aggacctcgg gcgctgcacc
      721 gtgaatgacc gacttgcgca ggtgcggcgc aggcaggtcg atgtctgccg cgatcttctc
      781 gacgacttcc tgggcttcgc gctccgggtc gtagcctccg accacgagcg ggaggccgta
      841 cgacatcggc cgtgcccaga cggtgacgag gtcgagggtg ccgccggtag ctcgcgcctg
      901 ctcaaccgcc cacgcagtcg ctttcgcgct ctcggccgag ccgtcgacgc cgacgacgat
      961 cagcgggcgg gtcacttctg tgtcggtact catgacattc tcctcagtct tgtggactca
     1021 agtttggacg ttcggcggtg gcgggcggca ctgccgttgg tccctgccta ccgggcagta
     1081 gtgctccctg cctcgggtgc tacctccgga ggggccccta ccgcaacccc tacgttcttg
     1141 gccggcgccg ccttcttagc cggcgccgat ttcttggcga ctttctttgc tgctgctgct
     1201 ttctttgctg gtgctgcttt cttcgcgggt gctgctttct tcgcgggtgc ggtcttcttc
     1261 gccggtgcgg tcttcttcgc cggtgccact gccttcgtcg ccgccgcgcg caccacgttc
     1321 ggaaggtcga tcatgcggca cggaatgtcg gtgttctcat tcacgaagta gctcgcgcag
     1381 atccggtgat aaccatcagc gatctgcaaa gggaatccgc gtagcaggtc gccgcgcacg
     1441 agcagcacgg gtgatagcgg ccgcgacttg cgtatctttc gcaggtcggc agcgacgtga
     1501 acgttgtcgg cggtgagcag tgcaagttgc gaggcgcgaa gcaggtcctt cgccttgtag
     1561 tgcgacacgg gcgcggcctg aagttgtgcg actaactgat cgcgcaatcc gtcgtctgcg
     1621 acgagcaaag acaggtagct ggacgccgca gggtaatcgt gcgcgtccgg tgcagtttcc
     1681 cagatcactt gcgccatcgg gatagcccca ttcgtcaggc ggtgctagta ggaggccgtg
     1741 caattcggcg tccctcatga tgccctaact tgcaccgagc gcacgaactt cccgccagca
     1801 gatcaggcga caccaacggc ttggccacgg gcctcagccc agagtgcgac gtaggcgttg
     1861 gcggcctcac tcgacgg
//
LOCUS       DASWVF010000228         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_261229, whole genome shotgun sequence.
ACCESSION   DASWVF010000228 DASWVF010000000
VERSION     DASWVF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261229"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGD55_04165"
     CDS             <1..411
                     /locus_tag="VGD55_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719462.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY0869568.1"
                     /translation="IGSAEAASSISAKPLARIAGRGAAGVDPDVFGIAPVAAAEQALR
                     RAGIGWGDLSVVELNEAFAAQSLACLGEWHELDPDLVNPHGGAIAIGHPLGASGARIL
                     GSLAHELHRRGGGWGLAAICIGVGQGLAVVLEAV"
     gene            428..1195
                     /gene="pcaH"
                     /locus_tag="VGD55_04170"
     CDS             428..1195
                     /gene="pcaH"
                     /locus_tag="VGD55_04170"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEY0869569.1"
                     /translation="MSSDRSDALPGALPDALTDALPQYERPRGLHPPLLSPAYRSTIL
                     RAPTQPLVLLPQNLTEVTGPLLGAERVRPGDNDLTRQHSGEPLGQRIIVRGRVLDSDG
                     RAVPDTLLEVWQANAGGRYRHIVDQWPAPVDPNFTGLGRAMTDSDGRYEFTTVKPGAY
                     PWNNHDNAWRPAHIHFSLFGLAFTQRLVTQMYFPGDPLFPFDPVLNSIPDPAARLRLV
                     SNFDLEASQPEWALAFTFDIVLRGSASTPFEAEVDDE"
     gene            1192..>1679
                     /gene="pcaG"
                     /locus_tag="VGD55_04175"
     CDS             1192..>1679
                     /gene="pcaG"
                     /locus_tag="VGD55_04175"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY0869570.1"
                     /translation="MSRIGLTPSQTVGPFLSIGLTWPDGAYAVTPSTPGARWVRGHLY
                     DGEGVPITDGLIETWQADPAGHFHHPDDVIGFAADPVAGFRGFGRSAAGPEGEWEICI
                     LKPGRVAASGGVLQAPHLDVTVLARGLLDRVVTRIYFGDEAEANAADPVLRSVPEHRR
                     GTL"
BASE COUNT          259 a          561 c          556 g          303 t
ORIGIN      
        1 atcggctctg cggaggctgc gtcgagtatc agtgcgaagc cgttggcgcg catcgcgggt
       61 cgcggcgcag cgggtgtcga ccccgacgtg ttcgggatcg cgcccgtcgc tgccgccgaa
      121 caggcgttac gtcgcgccgg aatagggtgg ggcgatctct ccgtcgtcga actgaacgaa
      181 gcgttcgccg cgcaatcgct tgcctgtctt ggcgaatggc atgagctcga tccagatctc
      241 gtcaaccctc atgggggtgc catcgcgatc gggcacccgc tcggcgcatc gggcgcgcgc
      301 atacttggca gccttgcgca tgaactgcac cggcgaggcg gtggttgggg tcttgcggct
      361 atctgcatcg gcgtcggcca gggcctggcc gtcgtcctcg aagccgtctg accaggagtt
      421 gatgcgcgtg tcgtcagata ggtccgacgc cctcccgggc gcgttgccgg acgcgttgac
      481 ggacgcgttg ccgcagtacg aacggccgcg cggcctgcat ccgccgctgc tgtcacctgc
      541 gtaccgcagc acgattcttc gagcgccgac gcagcccttg gtcttactgc cacaaaacct
      601 gaccgaggtg accggtccgc tgctgggtgc tgaacgggtg cgacccggcg acaacgacct
      661 cacccgtcag cacagcggag agccacttgg ccagcgcatc atcgtgcgcg ggcgggtgct
      721 cgacagcgac gggcgcgcgg tgcccgacac gttgctcgag gtctggcagg ccaacgcggg
      781 gggccgctat cgccacatcg tcgaccagtg gcctgcaccg gttgatccga acttcaccgg
      841 cctcggccga gcgatgaccg acagcgacgg acggtatgag ttcacgaccg tgaagccggg
      901 cgcctacccg tggaacaacc atgacaacgc ctggcgaccg gcgcacatcc acttctcgct
      961 gttcggcctt gcattcaccc agcgactcgt cacgcagatg tacttccctg gcgacccgct
     1021 gttccctttc gatcccgtgc tgaactcgat ccctgatccg gcggcccgcc tgcgcctggt
     1081 cagcaacttc gacctcgagg cgagtcagcc cgaatgggca ctggccttca cgttcgacat
     1141 cgtgttgcgt ggttcagcga gcaccccctt cgaggccgaa gtggacgacg aatgagccgg
     1201 atcggtttga cgccttcaca aacggtcggc ccgttcctgt ccatcgggct gacctggccc
     1261 gacggtgctt atgcggtgac gccgtcgact cccggagccc gctgggtacg gggacatctc
     1321 tacgacggcg agggtgttcc gatcaccgat ggtctgatcg agacttggca ggccgacccc
     1381 gcaggtcatt tccaccaccc cgacgacgtg atcggtttcg cggccgaccc ggttgccgga
     1441 ttccgaggtt tcgggcgtag cgcagcaggg cctgagggcg agtgggagat ctgcatcttg
     1501 aaacccgggc gggtcgcggc ttccggaggc gttttgcaag cgcctcatct tgacgtcacc
     1561 gtgctcgccc gcggcctcct cgatcgtgtc gtgacgcgca tctatttcgg cgacgaggca
     1621 gaagccaacg cagcagaccc ggtgttgcgc tcggtgccgg agcaccggcg tgggacgct
//
LOCUS       DASWVF010000229         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_465009, whole genome shotgun sequence.
ACCESSION   DASWVF010000229 DASWVF010000000
VERSION     DASWVF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465009"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1586)
                     /locus_tag="VGD55_04180"
     CDS             complement(9..1586)
                     /locus_tag="VGD55_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869571.1"
                     /translation="MKLELRGITKRFGSLVANDHIDLIVEPGEIHCLLGENGAGKSTL
                     MNVLYGLYAPDDGEILVDGVVTSFNGPGDAMAAGIGMVHQHFMLIPVFSVAENIMLGH
                     EDTRGPSGVGWLDRRAAKRKVADVSKRFGLAVPPDALVADLPVGVQQRAEILKALARD
                     AKVLVLDEPTAVLTPSEIDDLITVMRKLRDAGTSIVFISHKLKEVKAIGDTITVIRRG
                     RVVGQPSVSSSEQELASLMVGRPVSLTVDKATAQPGAIVLDVEGLRVVDAAGVVQVDE
                     VSFSVREGEIYAIAGVQGNGQTEVTECLVGLETPTAGRISVLGNDVTTSSVRDRLDLG
                     IGYVPEDRSHDGLVGSFTVEENLVLDMHQSPAFAGRFGLDMAAIDANGLARVAEFDVR
                     AESPQVAASTLSGGNQQKVVLARELSRPLKLLIVAQPTRGLDVGSMEFVHKRIVRERD
                     QRTAVVLISTELDEVLGLADRIAVMYQGRIIGELPGGSSAAAVGLLMAGSVAGASDAP
                     ETTTGAAIFEPGEGSAP"
BASE COUNT          328 a          614 c          581 g          321 t
ORIGIN      
        1 gacgaggctc atggagcact cccctctcca ggttcgaaga ttgcagcgcc ggtcgtggtc
       61 tccggtgcgt cggatgcacc cgcgacggat cccgccatca acagccccac tgcggccgct
      121 gaactgccgc cgggcagctc cccgatgatg cggccctggt acatgacggc gatgcgatcg
      181 gccagcccga gaacctcatc gagctcggtc gagatgagca ccacggcggt tcgctggtcg
      241 cgttcgcgga cgatgcgctt gtggacgaac tccatcgacc ccacgtcaag accgcgggtc
      301 ggctgggcga cgatcagcaa cttgagcggg cgcgacaatt cgcgggccag cacgaccttt
      361 tgctggtttc caccagacaa cgtgctggcc gccacctgcg ggctctcggc tcgcacgtcg
      421 aactcggcaa ccctggcgag accgttggca tcgatcgccg ccatgtcgag cccgaagcgg
      481 ccggcgaagg ctgggctctg gtgcatgtcg aggacgaggt tctcctcgac ggtgaagctg
      541 cccaccaatc cgtcgtgcga gcggtcttcc ggaacgtaac cgatcccgag gtcgagccgg
      601 tcgcgcaccg acgacgtcgt gacgtcgttg ccgagcacgc tgatccggcc ggccgtcgga
      661 gtttcaaggc cgacgagaca ctcggtgacc tcagtctgtc cgttgccctg cacgcccgcg
      721 atggcataga tctcgccctc gcgcacggag aaggacacct cgtcaacttg cacaacccct
      781 gcggcgtcaa cgacgcgcaa cccctcgacg tccaacacga ttgcaccagg ttgagccgtc
      841 gccttgtcga cggtcaacga gaccggtcgc ccaaccatga gcgacgcgag ctcttgctcg
      901 ctgctagata ctgacggctg gcccaccacg cggccgcgcc tgatgacggt gatcgtgtca
      961 ccgatcgcct tgacctcctt cagcttgtgg ctgatgaaga cgatcgaggt gcccgcgtca
     1021 cgcagcttgc gcatgacggt gatgaggtca tcgatctcgg atggcgtcag cacggccgtc
     1081 ggttcgtcga gaaccagcac cttggcgtcg cgggcaagcg ccttcaggat ctcggcccgc
     1141 tgctgcacac cgacaggtag atcggcgaca agagcatcag gcgggacggc gaggccgaac
     1201 cgcttcgata catcggcgac cttgcgcttc gccgcacgac ggtcgagcca gccgacgcca
     1261 ctaggaccgc gggtgtcttc gtggccgagc atgatgttct cggcgactga gaacactggg
     1321 atcagcatga aatgctgatg gaccatgccg atgccggccg ccatcgcatc gccagggccg
     1381 ttgaagctgg tgacgacgcc gtcgacgagg atctcgccgt cgtcaggggc gtacagcccg
     1441 tagaggacgt tcatcaaggt gctcttgcca gcgccgtttt cgcccagcaa gcagtgaatc
     1501 tcgcccggct cgacgatgag gtcgatgtga tcgttggcca cgagcgagcc gaaccgcttg
     1561 gtgatgccac gcagttcgag cttcaacgcg acctcagttc gctcagcacc accgcagtca
     1621 acaccaaagc cctaccgaat gggagaggtg ggcgggcccg tgatcgggcc cgcccacctc
     1681 tgcctacctc gactgcctgc ttacgttcta ctgcctactt accgtcttac ttgggctgcg
     1741 aaggcgacgt gatcgggacc gcgccgcttt cgatgtcggc cttgagcttg gtgagctcgg
     1801 cctgcacgct cgcgggaacc ttggaggcga agtcgtggaa cgga
//
LOCUS       DASWVF010000230         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_6370, whole genome shotgun sequence.
ACCESSION   DASWVF010000230 DASWVF010000000
VERSION     DASWVF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6370"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="acnA"
                     /locus_tag="VGD55_04185"
     CDS             <1..399
                     /gene="acnA"
                     /locus_tag="VGD55_04185"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720229.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0869572.1"
                     /translation="RYLGDGVPLVILAGKEYGSGSSRDWAAKGTALLGVRAVIAESFE
                     RIHRSNLIGMGVLPLQYAAGQTRESLGLTGEETFSIAGLAGADPLPREVTVHADGIEF
                     RATVRIDTPGEADYYRHGGIMQFVLRSLLA"
     gene            515..>1858
                     /gene="dxs"
                     /locus_tag="VGD55_04190"
     CDS             515..>1858
                     /gene="dxs"
                     /locus_tag="VGD55_04190"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0869573.1"
                     /translation="MSLLESVDGPEDLKRLDAAQLPQLAEEIRDFLVAAVAKTGGHLG
                     PNLGAVELTIALHRVFSSPTEPIIFDTGHQAYVHKLLTGRRGGFSSLRQRGGLSGYPN
                     RTESEHDWVENSHASTALSYVDGLAKAFAVRGEKRTVVAVVGDGALTGGMCWEALNNI
                     AAAKENSVVVLVNDNGRSYSETIGGLAEHLTTLRTNPRYEKALEKVKEVLGRTPLVGD
                     PLFDALHGIKRGLKDVLTPQGLFEDLGLKYVGPIDGHDIGAMESALARARKFGGPVIV
                     HCVTKKGFGYGPAENDELDHLHGPGAFDPLTGVELPKPASWTGAFSDEIVKIGATRPD
                     VVAITAAMLHPVGLAAFAEAYPDRIFDVGIAEQHAVTSAAGMAMGGLHPVFCVYATFL
                     NRAFDQVLMDVGLHGLPVTFVLDRAGVTGDDGASHNGMWDLSILSLVPGIRIALPR"
BASE COUNT          312 a          590 c          625 g          331 t
ORIGIN      
        1 cggtacctcg gcgatggcgt gccgctggtg atactcgccg gcaaggagta cggctccggg
       61 tcgtcacgag actgggcagc gaagggcacg gcactcctcg gcgtccgcgc cgtcatcgct
      121 gaatcgttcg agcggatcca ccgcagcaac ctgatcggca tgggggtctt gcccttgcag
      181 tacgcggccg ggcagaccag ggaatccctc ggcctgaccg gcgaggagac cttctcgatc
      241 gccgggctcg cgggcgccga cccgcttccg cgcgaggtca ccgtccatgc cgacggcatc
      301 gaattccggg cgaccgtgcg gatcgacacg ccgggggagg ccgactacta ccgccacggc
      361 ggcatcatgc aattcgtctt gcgctcgctc ctggcctgaa aggcgccgat cgggcagatt
      421 agagtgtctc agcggtcggg gccaggtgcc gggcgcgttc aggttcgcgg ttcaggttcg
      481 cggtgcaggt tcggtgacgg cgaggaggcg gcaggtgagt ttgctcgagt ccgtcgatgg
      541 gcctgaagac ctcaagcggc tcgacgcggc ccagttgccg caactcgctg aggagatccg
      601 cgacttcctg gtcgcggcgg ttgccaagac cggcggccac ctcggcccca atctcggcgc
      661 ggttgagctc acgattgccc tccaccgggt tttcagttca ccgaccgagc cgatcatctt
      721 cgacaccggg catcaggcct acgtgcataa gcttttgacc ggacgccggg gtggtttctc
      781 gagcctgcgc cagcgcggtg gcctctcggg ctaccccaac cgcacggaat ccgagcacga
      841 ctgggttgag aactcccatg cgtcgacggc cctgagttac gtcgatggtc tcgcgaaagc
      901 gttcgccgtg cgaggtgaga agcggaccgt cgtagccgtt gtcggcgacg gtgcgctaac
      961 cggcggcatg tgctgggaag cgctcaacaa catcgctgcc gcaaaggaaa actcggtcgt
     1021 cgtgctcgtc aacgacaacg gtcgctcgta ctcggagacg atcggcgggc ttgccgaaca
     1081 cctcacgaca ctgcgcacca acccgcggta cgagaaggca ctcgagaaag tgaaagaagt
     1141 actcgggcgc acgccgctgg tcggggatcc gctgttcgat gcgttgcacg gaatcaagcg
     1201 cgggctcaag gatgtcctta cgccacaagg acttttcgaa gatctcggct tgaagtacgt
     1261 cggcccgatc gacggtcacg acattggcgc gatggagtca gctcttgcgc gggcacgtaa
     1321 gttcggcggc ccggtcatcg tgcattgcgt cacgaagaag ggcttcggtt acggccccgc
     1381 cgagaacgac gagcttgacc acctgcatgg tcccggcgca ttcgacccgc tgaccggcgt
     1441 cgagttgccc aagccggcga gctggactgg cgcgttctcc gacgagatcg tgaagatcgg
     1501 cgcgacccga cctgacgtcg tcgcgatcac ggccgccatg ctgcacccgg tcgggctcgc
     1561 ggcgttcgcc gaggcgtatc cagaccgcat cttcgatgtc ggcatcgcag aacagcacgc
     1621 ggtgacgtcg gccgccggca tggcgatggg tggcctgcac ccggttttct gcgtgtacgc
     1681 gacgttcctg aaccgtgcgt tcgaccaagt tctgatggac gtcggattgc acggtctgcc
     1741 cgtgacgttt gtgctcgacc gggctggggt taccggcgac gacggtgcaa gccacaacgg
     1801 gatgtgggac ctttcgatcc tgtcgctggt gcctggaatc cgcatcgcgt tgccacgt
//
LOCUS       DASWVF010000231         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_337502, whole genome shotgun sequence.
ACCESSION   DASWVF010000231 DASWVF010000000
VERSION     DASWVF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337502"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGD55_04195"
     CDS             <1..551
                     /locus_tag="VGD55_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0869574.1"
                     /translation="RHAGITNIVWASSETVLGLPFGTPPPYIPVDEEYPARPESTYSL
                     VKHLEEQMARQLCRWDKDLKMIGLRFSNVLDPTDYIAFPSYDADPSLRKWNLWAYIDS
                     RDGAQAIRKALEYDVKGCDVFVIASPDTVMSRPNSELIGQIFPDVELRHEIGEHETLL
                     SIDKARRVLGFDPQFSWRTESA"
     gene            complement(559..>1802)
                     /locus_tag="VGD55_04200"
     CDS             complement(559..>1802)
                     /locus_tag="VGD55_04200"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869575.1"
                     /translation="SVAAVSFPVRTIEAAELRDWFDALSSAYFLTADPQAIADFRRPQ
                     LELDRAWAAYDGDRIIATLRTFATELTLPGGAQLPADAVTSVATMPTQRRRGALTAMM
                     TASLDAAVKHGDPLSILIAARWPIYGRYGFGPATELAEYSIDTVIADFGKLGRDGPLE
                     FVDAATGRALAPALFDRFRRVQSGAISRTDIDFDRDFGKAVMPGRTPWAGRVVVHRPA
                     PGAEIDGFLRYHVDERWENLIPKSVAVVDDLVAITQSAYAALWKLCCDLDNVASVTAA
                     NRSVDEALPWMLVDARAMVQVRRSDIVWLRLLDVETALSARRYLATGSLVIEVRDAMG
                     YATGRYELNGGPDAASCTRTTKSADIELTATTLGSAYLAGMSLQTMAAAGLVIEHRAG
                     AVALADAMFRWNVVPWCNTWF"
BASE COUNT          325 a          574 c          597 g          306 t
ORIGIN      
        1 cacgtcacgc tggcattacg aatatcgtgt gggcgtcgag cgaaacggtt ctcgggctgc
       61 ctttcggcac gccgccgccc tacattccgg tcgatgagga atacccagcg cggcccgagt
      121 cgacgtactc gctggtcaaa cacctcgaag agcagatggc tcgccagttg tgccggtggg
      181 ataaggattt gaagatgatc ggcctgcgat tctctaacgt gctcgacccg accgactaca
      241 ttgcatttcc gagctacgac gccgatccgt cattgcgcaa atggaacctg tgggcttaca
      301 tcgattcacg cgacggcgca caggctattc gcaaagctct ggaatacgac gtcaaaggct
      361 gcgacgtatt cgtgatcgcg tcgcccgaca cggtgatgtc acgacccaac agcgaactca
      421 tcggacagat atttccggac gtcgaattgc ggcacgagat cggtgagcac gagaccttgt
      481 tgtcgatcga caaagcacgt cgcgtgctgg ggttcgaccc gcagttcagc tggcgcacgg
      541 aaagcgccta gaaaccggtc agaaccaggt attgcaccaa ggaacgacgt tccatctgaa
      601 catcgcgtcg gccagcgcaa ccgcaccggc ccggtgttcg atcaccagcc cggcagccgc
      661 catggtctgg agggacatgc cggccagata cgcagagccg agtgtggtgg cagtcagctc
      721 gatgtcggcc gactttgtgg tgcgcgtgca gctcgcggcg tccggcccgc cgttcagttc
      781 gtaccgaccc gtcgcgtacc ccatcgcatc gcgcacctcg atcaccaggc tgcccgtcgc
      841 gaggtagcgg cgagccgaca gggccgtctc gacatcgagt agccgcaacc acacgatgtc
      901 gctgcgtctg acctgcacca tcgcccgcgc gtccaccagc atccacggga gggcttcgtc
      961 gacgctgcgg ttcgctgcgg tcacgcttgc gacgttgtcg aggtcgcagc acagcttcca
     1021 taaggcggcg tacgcggatt gcgtgatcgc gacgaggtcg tcgacgaccg cgacgctctt
     1081 cggaatcagg ttttcccagc gttcgtcgac gtggtaccgc aagaagccgt cgatctcggc
     1141 gccgggcgcg ggccggtgaa ccacgaccct gccggcccac ggcgtacggc ctggcatgac
     1201 ggccttgccg aagtcacgat cgaagtcgat gtcggtgcgg ctgatcgcgc ccgactgcac
     1261 gcggcggaag cggtcgaaca aggccggcgc gagggcgcgt ccggttgcgg cgtcgacgaa
     1321 ctcgaggggt ccgtcgcggc cgagcttgcc gaagtcggcg atcaccgtgt cgatggagta
     1381 ctcggcgagt tcggttgctg gcccgaagcc gtaccgcccg tagatcggcc agcgcgcggc
     1441 gatcaaaatg ctcagcggat cgccgtgctt cacagcggca tcgagggaag ccgtcatcat
     1501 cgcggtgagc gcgccgcggc gccgctgggt cggcatcgtg gcgacgctcg tcacggcatc
     1561 tgccggcaat tgggcgccgc cgggcagtgt cagttcggtc gcgaaggtgc gcagcgtcgc
     1621 gatgatgcgg tcgccgtcgt aggccgccca cgcacggtcg agttcgagct gaggacggcg
     1681 gaagtcggca atggcctgcg ggtcggcggt cagaaagtag gcgctggaca gcgcgtcgaa
     1741 ccagtcgcgg agctcggccg cctcgattgt cctgaccgga aagctcacgg ccgcgacgct
     1801 ac
//
LOCUS       DASWVF010000232         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_184724, whole genome shotgun sequence.
ACCESSION   DASWVF010000232 DASWVF010000000
VERSION     DASWVF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184724"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1578
                     /gene="glnA"
                     /locus_tag="VGD55_04205"
     CDS             154..1578
                     /gene="glnA"
                     /locus_tag="VGD55_04205"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0869576.1"
                     /translation="MFTSADEVLGYIRENDVKSVDVRFCDLPGIMQHFTVPVESFDEK
                     IFTDGLGFDGSSIRGFQAIHESDMLLLPDPTSGVLDPFRTEKTLNLTFFIHDPLTGEA
                     YSRDPRNVAKKAETYLASSGIADTVYFGPEAEFYIFDNVRFETNQHSSYYYIDAEAGV
                     WNTGRAEEGGNRGYKVRNKGGYFPVPPADHYADLRAEMSRELINAGLTVERQHHEVGS
                     AGQAEINYKFAPLLKAADDLLTFKYIIKNVAWRNGKTVTFMPKPVFGDNGSGMHCHQS
                     LWKDGAPLFYDELGYAGLSDMARHYIGGLLKHAPSLLAFTNPTVNSYHRLVPGFEAPV
                     NLVYSQRNRSACIRIPITGSNPKAKRLEFRVPDPSCNPYLAFSAMMMAGLDGIKNKIE
                     PMDPVDKDLYELPPEEHASVPQVPGSLPEVLAALEVDQDYLLAGGVFTQDLIDTWIEY
                     KRKNEIDAMRLRPHPHEFELYFDI"
     gene            2007..2237
                     /locus_tag="VGD55_04210"
     CDS             2007..2237
                     /locus_tag="VGD55_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869577.1"
                     /translation="MACLRIYDAVHLLANLGTAPLAVDAADAAYAYVHGNAPDAGDAE
                     LQTLLTSPTPSYDDLGPAAAYCQRIGVANPTT"
     gene            2385..>2597
                     /locus_tag="VGD55_04215"
     CDS             2385..>2597
                     /locus_tag="VGD55_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869578.1"
                     /translation="MSKAYQVLARLIAGLVVLQAAAIAYGAFAIDKVIEKAKDHGDTV
                     ASASTKLDGGAGYGLHALIGTLIIPLA"
BASE COUNT          492 a          876 c          799 g          430 t
ORIGIN      
        1 gcattacacg ggactgcact ccgtgtacac gaagcgcccg cccgactcgc gcacgttcag
       61 ggggcgtaac gcgcaggtaa ctcacgagac acgcgcggga aatcggtctg ccttaacgtg
      121 cctgacgttt ccagtcgtcc aaggaggaga tacatgttca ccagcgccga cgaggtcctg
      181 gggtacatca gggagaacga cgtcaagtcc gtcgacgtgc ggttctgcga cctgcccggc
      241 atcatgcagc acttcactgt tcccgttgaa tccttcgacg agaagatctt caccgacggc
      301 ctcggtttcg acggctcgtc gatccgtggc ttccaggcga tccacgagtc ggacatgctg
      361 ttgctgcccg acccgacgtc gggtgtgctc gacccgttcc gcaccgagaa gacgctgaac
      421 ctgacgttct tcatccacga cccgctgacg ggcgaggcgt acagccgcga cccgcgcaac
      481 gtcgcaaaga aggccgagac ctacctcgcg agcagcggca tcgccgacac cgtgtacttc
      541 ggtcccgagg ccgagttcta cattttcgac aacgttcggt tcgagaccaa ccagcactcg
      601 agctactact acatcgacgc cgaggcgggt gtctggaaca ccggtcgcgc tgaagaaggc
      661 ggcaaccgag gctacaaggt ccgcaacaag ggcggctatt tcccggtgcc gccggccgac
      721 cactacgccg acctgcgcgc cgagatgagc cgcgagctga tcaacgcggg gctcaccgtc
      781 gagcggcagc accacgaggt gggtagcgcg ggccaggccg agatcaacta caagttcgcg
      841 ccactgctga aggcagccga cgacttgctg acgttcaaat acatcatcaa gaacgtcgcg
      901 tggcgcaacg gcaagacagt gacgttcatg ccgaagccgg tcttcggcga caacggctcg
      961 ggcatgcact gccaccagag cctgtggaag gacggcgcgc cgctcttcta cgacgagctt
     1021 gggtacgcgg gtctttcaga catggcgcgg cattacatcg gcggactcct caagcacgcg
     1081 ccctcgctgc tcgcgttcac caacccgacg gtcaacagct accaccgcct ggtgcccggt
     1141 ttcgaggctc ccgtcaacct ggtctatagc cagcgcaacc ggtcggcctg catccgaatc
     1201 ccgatcaccg gcagcaaccc gaaggcgaag cgcctggagt tccgggtgcc cgacccgtcg
     1261 tgcaacccct acctcgcgtt ctcggcgatg atgatggccg gcctcgacgg catcaagaac
     1321 aagatcgagc cgatggaccc ggtcgacaag gacctctacg agttgccgcc tgaggagcac
     1381 gcgagtgttc cccaggttcc cggctcgctg cccgaggtac tcgccgcact tgaggtcgac
     1441 caggattatc tgctcgccgg tggcgtcttc acccaagacc tcatcgacac ctggatcgag
     1501 tacaagcgca agaacgagat cgacgcgatg cggttgcgcc cgcacccgca cgagttcgag
     1561 ctctacttcg acatctaggc cggacgccga actgcggctg atttacgcac gttgcggcac
     1621 ccgcccgagt ccgcagtgag agtccgcacg agcagccttc tcgtgcggac tcatcgcttc
     1681 ctgacggccg gttggccgcc acccgagaat gggcaattat ggtcgggtgg gagcgcaact
     1741 gagggcccgc gttagcctac ggttgagcca tgtgggcacc tcgacgcctg gtttggctag
     1801 cgctcgcggg atccctcctt tgggcttgcg acacatccag gcacggtggt gccgcggatt
     1861 caccacagcc gtccacgcag acggttgtgc gcacatcctc gctagcgtcg acttcgccgg
     1921 cgaaggtcgc cagtacggcg agcgcgcccg ccgaacccgc ttcgtcggcg cccccttccg
     1981 agacaccacc tacgccgggg gacaccgtgg cgtgccttcg catctacgac gctgtgcacc
     2041 tgctagcgaa cctcgggacg gccccgctag ccgtggacgc ggcggatgcg gcctatgcgt
     2101 acgtgcacgg caacgccccc gacgcgggag atgcggagct gcagacgctg ctgacgtccc
     2161 caacgccctc ctacgacgac ctaggcccag cggccgcgta ctgccagcgc atcggcgtgg
     2221 cgaatccaac tacctagcac cgtcgccgga acaaatgacg acggtttggt cggcccacct
     2281 gagccgcgac ccgtgagccc aaccggtctg gttcgtagcg attccggaac gattgcccac
     2341 taccttcggc cgcatgcggt gtccgagcgt ccgtgggagg accgatgagt aaggcgtacc
     2401 aagttctggc gagactgata gcgggtctgg tcgttttgca ggcggctgcg atcgcctacg
     2461 gggcgttcgc catagacaag gtcatcgaga aggcgaaaga ccacggcgac acggtcgcca
     2521 gcgcatcgac gaagctggac ggtggcgcgg gctacgggtt gcatgcactg atcggcaccc
     2581 tgatcatccc gctcgcc
//
LOCUS       DASWVF010000233         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_261407, whole genome shotgun sequence.
ACCESSION   DASWVF010000233 DASWVF010000000
VERSION     DASWVF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261407"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..978
                     /gene="thyX"
                     /locus_tag="VGD55_04220"
     CDS             214..978
                     /gene="thyX"
                     /locus_tag="VGD55_04220"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY0869579.1"
                     /translation="MSETPGVVLPETAAAALQPRFRSDVTVELIKHVASDADVLFAAR
                     VSTKGESSLDDVNADPSRSQGLLNYLMRDRHGTPFEHNSMTFFVSAPIFVFREFMRHR
                     IGFSYNEESGRYRQLEPVFYVPGPDRLLVQEGKPGRYVFTPGTAEQHEIAQRATKAAC
                     EQAYAAYLEMLSAGIAREVARGVLPVATFSSMYVTCNARSLMALLSLRTKHEDSRFPS
                     FPQREIEMVAEKMEAEWAALMPLTHAAFEAHGRVSP"
     gene            1020..>1911
                     /gene="dapA"
                     /locus_tag="VGD55_04225"
     CDS             1020..>1911
                     /gene="dapA"
                     /locus_tag="VGD55_04225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0869580.1"
                     /translation="MTTPDSGTTTPPPTPPFGRVMTAMVTPFTADGGLDLDGAQQLAS
                     YLVDHGNDGLVINGTTGESPTTTDEEKERLLRAVLEAVGERAKVVAGVGTNDTRHTIE
                     LAHGAEKVGAHGLLVVTPYYSKPPQAGLLAHFRQIADATGLPNMLYDIPGRTATPIET
                     ETLVRLAEHDKIVAVKDAKGDLEATSWVLRRTDLAYYSGDDKVTLPLLSVGAVGVVGV
                     PTHLFGVETGSMIAAYLRGDVAGALAAHRQLLPVFTGFFRTQGVILTKAALRLAGLPG
                     GPVRPPLVDATDAQIEQLRRD"
BASE COUNT          313 a          645 c          630 g          323 t
ORIGIN      
        1 tctgagggcg caagctgcgc ggagcaggct gcgggcacag gcagtcgcgg cccctacgag
       61 acgggcgtcg cggcggtcgg cggtctggcc tgcgcggtta cgtctggacg ccggggcggc
      121 gcgccgagct cagcccctcg ccgtcccgtc cggtagccgt gtcacgactg tccgcgtccg
      181 gccggcggtc gactgctgac tatggtgtcc gtcatgtccg agacaccagg ggtggtcctt
      241 cccgagaccg ccgcagctgc gctccagccg cggtttcgca gtgacgtcac ggtcgagctg
      301 atcaagcatg tggcgagtga cgcagacgtg ctgttcgcgg cgcgcgtctc aaccaagggc
      361 gagtcgtcgc tcgacgacgt caacgctgat ccctcgcggt cgcagggctt gctcaactac
      421 ttgatgcgcg atcgccacgg cacgccgttc gagcacaact cgatgacgtt tttcgtgtcc
      481 gcgccgatct tcgtgttccg cgagttcatg cggcaccgca tcggcttcag ctacaacgaa
      541 gaaagcgggc gttatcggca gctcgagccg gtgttctacg tgcctgggcc tgaccgcctt
      601 ctcgttcagg agggcaagcc agggcgttac gtcttcacgc caggcacggc ggaacagcac
      661 gagattgctc agcgggcaac gaaagcagcg tgcgagcagg cttacgccgc gtacctcgaa
      721 atgctgtcgg ccggcatcgc gcgtgaagtc gcgcgcgggg tgctgccggt ggcaacgttt
      781 tcgtcgatgt atgtcacctg caacgcgagg tcactgatgg cactcctgtc actgcgcacg
      841 aagcacgaag acagccgctt cccgagcttc ccccaacgcg agatcgagat ggtcgccgag
      901 aagatggagg ccgagtgggc agcgctcatg ccgctgactc acgccgcgtt tgaagctcac
      961 gggcgggtca gcccataacg ggattcgttg ccgggcgccc taccggtacc gttgctgcca
     1021 tgactacccc ggattcgggt acgaccacac cgccccccac accgccattt ggtcgtgtca
     1081 tgacggcgat ggtgaccccg ttcaccgccg acggagggct cgaccttgac ggcgcccagc
     1141 agttggcgag ctatctcgtc gaccacggca acgacggcct cgtcatcaac ggcaccaccg
     1201 gtgaatcacc gacgaccacc gacgaggaga aggaacggtt gctgcgggcc gtgctcgagg
     1261 ccgtcggtga gcgggccaag gtcgtcgccg gagttggcac gaacgacaca cgtcacacaa
     1321 tcgagctcgc ccatggcgct gagaaggtgg gcgcgcacgg gctcctcgtc gtcacgccgt
     1381 actactccaa gccaccgcag gcgggactgc tggcgcactt tcgtcagatc gccgatgcca
     1441 ccggcctgcc gaacatgttg tacgacatcc caggccgtac ggcgacgccg atcgagaccg
     1501 agacgctcgt ccgcctcgcc gagcacgaca agatcgtcgc ggtgaaggac gccaagggcg
     1561 acctcgaagc cacgtcctgg gtgctgcgtc gcaccgacct ggcctactac tccggtgacg
     1621 acaaggtgac tctcccgctg ctgtcggttg gggcggtcgg cgtggtcggc gtcccgaccc
     1681 atctgttcgg tgtcgaaacg ggctcgatga tcgccgccta cctgcgcggt gacgtcgccg
     1741 gcgcgctggc ggcccaccgt cagttgcttc cggtgttcac cgggttcttc cgcacgcagg
     1801 gcgtgatcct gacgaaggcg gcccttcggc tggcaggtct ccccggtggc ccggtccgtc
     1861 cgccgctcgt cgacgcgacc gatgcacaga tcgaacagtt gcgccgcgac a
//
LOCUS       DASWVF010000234         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_312006, whole genome shotgun sequence.
ACCESSION   DASWVF010000234 DASWVF010000000
VERSION     DASWVF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312006"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VGD55_04230"
     CDS             complement(<1..695)
                     /locus_tag="VGD55_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0869581.1"
                     /translation="MSTSVGSVKRLFVGRPLRSQQLHETLLPKWLALPIFASDPISSV
                     AYATEEIVLVLAVGGGAAALTYAKNVAAAVALLLVIVTLSYRQICKAYPNGGGAFAVS
                     LDNFGENAALTAGSALLVDYVMTVAVSVVSGVVAITSAFPSLHRHAVALSVGFVVLLM
                     VANLRGVKESGRTFAVPTYSFIALTFVMFGVAAVKAANSGLPRASTAGQTLTHSHLAR
                     LALILLTLKAFASG"
     gene            783..1949
                     /locus_tag="VGD55_04235"
     CDS             783..1949
                     /locus_tag="VGD55_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0869582.1"
                     /translation="MAGPPYGVIWSGQGHTRLVIWSLARVLASGIGRRRLVGAFVLEA
                     LGLPILTICLAAMRSHLYLADDLLLYLAAVIGVTLVGGFVPAIAAAVASSLLLNWYFT
                     PPVHTWTIDSPQNLLALVLFVAVAVTVGSVVHLAARRALLASALATQAARAQGLAEGN
                     RIRTTLLAAVGHDLRTPLASVKASVSTLRQTDVTWSAQDQAELLATIEEGADRLNALI
                     GNLLDMSRIQGGAVQLFVRPTSLEEVAPLVVEAVDGGALLRVEMSEDLPLFRADPGLL
                     ERALANLVVNALRYSPPDQPPTLAGRVVHNGAADGVVQIEVIDHGPGVPADQWERIVA
                     PFEQLGDQRNGNGVGLGLAVTKGFVEAMGGILSADATEGGGLTMRIELPVSAPR"
     gene            1946..>2201
                     /locus_tag="VGD55_04240"
     CDS             1946..>2201
                     /locus_tag="VGD55_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869583.1"
                     /translation="MTSVLVIDDEASILRALRINLAARDYTVTTASDGASGLAAMARV
                     RPDVVILDLGLPDMEGTDVIRGIRGWSTVPIIVLSANEQEL"
BASE COUNT          378 a          703 c          743 g          377 t
ORIGIN      
        1 cccgaagcga aggccttcag cgtgaggagg atcagtgcaa gcctggcgag atggctgtgc
       61 gtcagggtct ggccggcggt cgatgcgcga ggtagcccgc tgttggccgc cttgacggca
      121 gcaactccga acatgacgaa cgtcaaagca atgaacgagt acgtcgggac ggcgaacgtg
      181 cggccggatt ctttgacacc acgcaagttc gcgaccatga gcaaaacgac gaagccgacc
      241 gagagcgcga ccgcatgccg gtgcagcgac gggaacgcgc tggtaatggc gacgacgccg
      301 gaaacgaccg acacagcgac cgtcatgacg tagtcgacga gcagtgcact gccggctgtc
      361 agcgccgcgt tctcgccgaa gttgtcgagg ctgaccgcaa acgcgccgcc gccgttgggg
      421 taggccttac agatctgccg gtacgacaag gtgacgatca cgagcagcaa cgccacggct
      481 gcggcgacgt tcttggcgta ggtgagagcg gccgcgcccc cgccgaccgc aaggaccagg
      541 acgatctcct ccgtcgcata ggcgaccgaa gagatcgggt cggacgcaaa tatcggcagc
      601 gccagccatt tgggtagcag cgtctcgtgg agttgctggc tccgaagggg acgaccgacg
      661 aagagtcgct tcacgctgcc gacggaggtg ctcactgact tcacgctagt gcggcgcgcg
      721 cgagttgagc gtcaccaggg tgtcaagatc acccgcggaa ccgtcaagga accgtcaaga
      781 tcgtggcggg gccgccctac ggggtgatct ggtcgggaca gggccacact cgtctcgtga
      841 tctggagtct cgctcgcgta cttgcgtcgg gtatcggccg ccgccgtctc gtgggcgcgt
      901 tcgtgctcga ggcgctcggt ctcccgatcc ttactatctg tctggcggct atgcgatctc
      961 acctttatct tgccgatgac ctgctgcttt acctggcagc cgtcatcggc gtcacgctcg
     1021 tcggcggctt cgtgcctgcc atcgctgcag cggtcgctag cagcctgcta ctcaactggt
     1081 acttcacccc gccggtacac acctggacga tcgactcgcc ccagaacctg ctcgcgctgg
     1141 tgctcttcgt ggccgtggcc gtcaccgtcg gcagcgtcgt gcacctcgcg gctcgccgcg
     1201 ccttgctcgc atccgcgctc gcgacgcagg cagcgcgcgc gcaaggcctt gcggagggca
     1261 accggatccg caccacgctg ctcgccgcag tcggtcacga cctgcgcact ccgttggcat
     1321 cggtgaaagc cagcgtcagc acgttgcgcc aaaccgacgt cacctggtcg gcgcaagacc
     1381 aagccgagtt gctcgcgacg atcgaggaag gtgcggatcg gctcaatgca ctgattggca
     1441 acctgctcga catgagccgg atccaggggg gtgcggtgca gctgtttgtg cgaccgacct
     1501 cattggaaga ggttgcgcct ctcgtggtcg aggcggtcga cggcggtgcg ttgctgcgtg
     1561 tcgagatgtc tgaggacctg ccgttgttcc gagccgatcc cggcctgctc gagcgagcgt
     1621 tggccaacct tgtggtcaac gcgttgcggt actccccacc agaccagccg cccacgctcg
     1681 ctggtcgcgt cgtgcacaac ggggccgcag acggcgtcgt gcagatcgag gtcatcgatc
     1741 acggccccgg cgtgccggct gatcagtggg agcgcatcgt cgcacccttc gagcagcttg
     1801 gtgaccagcg caatggcaac ggtgtggggc tgggcctcgc ggtcaccaag ggcttcgttg
     1861 aagcgatggg cgggatattg agtgccgatg cgaccgaagg cggtggcctg acgatgcgga
     1921 tcgagctgcc ggtgagcgcg ccccgatgac aagcgtcctg gtcatcgacg acgaggcctc
     1981 gatcctgcga gcgctgcgca tcaacctcgc cgcccgcgac tacacggtga cgacggcctc
     2041 cgacggcgcg tcaggcctgg ctgccatggc gagggtgcga cccgatgttg tgatcctgga
     2101 tctcgggcta cccgacatgg agggcaccga cgtcattcgc gggatccgcg gatggtcgac
     2161 cgtgccgatc atcgtgctgt cggcgaatga gcaggaacta c
//
LOCUS       DASWVF010000235         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_312018, whole genome shotgun sequence.
ACCESSION   DASWVF010000235 DASWVF010000000
VERSION     DASWVF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312018"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGD55_04245"
     CDS             complement(<1..251)
                     /locus_tag="VGD55_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869584.1"
                     /translation="MAFSTTLLVVRWAYRRRQVRGASDPTAILVGLAVGFLSFVGGWL
                     LIDVYLLTRTAWRATSTIPGRVHVNAVRKWPLGNNHRPS"
     gene            complement(335..589)
                     /locus_tag="VGD55_04250"
     CDS             complement(335..589)
                     /locus_tag="VGD55_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869585.1"
                     /translation="MSSIAVLVEDHRMQIHPAGLGAGEVAVRTGLVTISASTPQPPLT
                     HARHPTSTRAPSTTCTFWAVIVTASLTRSNWLALPPESEF"
     gene            579..746
                     /locus_tag="VGD55_04255"
     CDS             579..746
                     /locus_tag="VGD55_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869586.1"
                     /translation="MDDKQTLTVGSSSIRVGCLSVEQQLRFHSGYELRDVGLSDLALL
                     RQLEPDAIPES"
     gene            872..1777
                     /gene="dapA"
                     /locus_tag="VGD55_04260"
     CDS             872..1777
                     /gene="dapA"
                     /locus_tag="VGD55_04260"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0869587.1"
                     /translation="MPSQDLAAANPPFGRVLTAMVTPFTADGGLDLDGAQRLATYLVD
                     HGNDGLVINGTTGESPTTTDDEKERLLRAVVEAVGDRAKVVAGVGTSDTRHTIELAHA
                     AEKAGAQGLLVVTPYYSKPPQAGLLAHFRQIADATGLPNMLYDIPGRTATPIETDTLV
                     RLAEHDKIVAVKDAKGDLEGTSWVLKRTDLAYYSGDDKLTLPLLSVGAVGVVGVPTHL
                     FGIETAAMIAAYLRGNVAGALALHRQLLPVFTGFFRTQGVILTKAALRLAGLPGGPVR
                     PPLVDATDEQIEQLRRDAADAGTAL"
     gene            1774..>2164
                     /locus_tag="VGD55_04265"
     CDS             1774..>2164
                     /locus_tag="VGD55_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0869588.1"
                     /translation="MSYPHAGLSAPGPLAENGLRIVALGGLGEVGRNMTVFEYGGRLL
                     IVDCGVLFPDPEQPGIDLILPDFDYIRARLDKVEAIVLTHAHEDHIGAIPYLLRERPD
                     IPLVGSDLTLAFIEAKLVEHRIKPYTLP"
BASE COUNT          366 a          728 c          698 g          372 t
ORIGIN      
        1 cttgacggtc gatggttgtt gccgaggggc cacttccgaa ccgcgttcac gtgaacgcgg
       61 cccggaatcg tgctggtcgc ccgccacgcg gtccgcgtca gcaggtacac atcgatgagc
      121 agccaaccac ccacaaatga caggaagccc accgctagcc cgaccaggat cgccgtcggg
      181 tcgctagctc cgcgcacttg gcggcgcctg tatgcccatc gaacgacgag cagcgtcgtc
      241 gagaacgcca aggcaacgat gcccggcgct tggcgccaaa gtcacggcat ccagctagct
      301 cttccggtgt ctggcgaagc gctgccggtc gacctcagaa ttccgattcc ggcggtagcg
      361 cgagccagtt agaacgggtg agcgacgcgg tgacgatgac cgcccagaat gtgcatgtcg
      421 tgctcggagc cctcgttgat gtcgggtgcc gggcgtgggt gagcggtggt tggggtgttg
      481 acgcgctgat ggttaccaga cccgtccgca ccgcgacctc gcctgcgccg aggccggctg
      541 ggtggatctg catccggtgg tcttcgacga gaacggcgat ggacgacaag cagacgttga
      601 cggtgggttc ttcgtctatt cgcgtgggct gcctgtccgt cgagcagcag ttgcgatttc
      661 atagcggata cgagcttcga gacgttggcc tcagcgatct tgcgctgcta cgccagcttg
      721 agccggacgc gatccccgag tcctgacttc gcagcaaccg gtgatcagca caatcgcccg
      781 agcaactcag ggggccgacc cgaggctccg gcgccccgca gccgccaagt aatctccggc
      841 gcggcgttcg gcgtccggta ccgttacggg catgccgtcc caggacctcg ctgccgcgaa
      901 cccaccgttt gggcgcgtgc tcaccgcgat ggtgacgccg ttcaccgccg acggtggtct
      961 cgacctcgac ggcgcccagc ggctcgcgac ctatctggtt gaccacggca acgacggcct
     1021 cgtcatcaat gggaccaccg gagagtcgcc caccacgacc gacgacgaga aggagcgatt
     1081 gctccgcgcg gttgtagaag ctgtcgggga tcgggcaaag gtcgtcgctg gcgttggcac
     1141 cagtgacacc cggcacacca tcgagctcgc gcacgccgcc gaaaaggctg gcgcgcaggg
     1201 tcttttggtc gtcacccctt actactcgaa gccaccgcag gccggtttgc tcgcacactt
     1261 ccgccagatc gccgacgcca ctgggctgcc gaacatgctg tacgacatcc ccgggcgcac
     1321 ggcgacgccg atcgagaccg acacgctcgt caggctcgcc gaacacgaca agatcgtggc
     1381 ggtgaaggac gccaagggcg acctcgaggg aacgtcctgg gtgttgaagc gcactgacct
     1441 cgcgtactac tcgggcgacg acaagctcac gttgccgctg ctgtcggtcg gggccgtcgg
     1501 cgtggtcggc gtcccgaccc atctgttcgg tatcgagact gccgcgatga ttgccgccta
     1561 cctgcgcggc aacgttgccg gtgcgctcgc gctgcaccgc caactgctgc cggtgttcac
     1621 cggcttcttc cgcacccagg gagtcatctt gacgaaggcc gcgctgcggc tcgcgggtct
     1681 gccgggcggg cccgtgcggc cgccgctggt cgatgcgact gacgagcaga tcgagcagct
     1741 gcgccgtgac gcggctgacg ccgggaccgc cttgtgagct acccgcacgc cggactctcg
     1801 gcgccaggtc cactcgcgga gaatggcctt cggatcgtgg ctctgggcgg actcggcgaa
     1861 gtcggtcgca acatgaccgt cttcgagtac ggcggccggc tactgatagt cgactgcggc
     1921 gtgctgttcc ccgatcccga acagcctggc attgatttga tcctgcccga cttcgactac
     1981 atcagagctc gcctcgacaa ggtcgaggcg attgttctca cacatgcgca cgaagaccac
     2041 atcggtgcga tcccttacct gttgcgcgag cgacccgaca ttccactcgt cggctccgac
     2101 ctcacgctcg cgttcatcga ggcgaagctc gtcgagcacc ggatcaagcc atacacactg
     2161 ccgg
//
LOCUS       DASWVF010000236         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_31935, whole genome shotgun sequence.
ACCESSION   DASWVF010000236 DASWVF010000000
VERSION     DASWVF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31935"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VGD55_04270"
     CDS             complement(<1..963)
                     /locus_tag="VGD55_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0869589.1"
                     /translation="MPRPAKAGRFDIADIVRAHRSALEASVRLTTAQKRVLTDIAQCR
                     TAALGGHVDRCLTCGYEHPSYNSCRNRHCPKCPALAQEKWIEERRAAQLDVRHFHVVF
                     TLPAELRPLARFAPSIVYNALFQASQRTLLEFGQSRLGGTLGATLVLHTWTRALHFHP
                     HIHAIVTGGALSTDGARWHDADRRYLFPVRAMGNVFRAKMLALLRRADRRHAFTGFAG
                     FDDPDGFDRLMRRAARQTWNVYSKAPFKMGRHVLDYLGRYTHRVGIANSRLVKVTHDI
                     VRFRTRNGAVEALAPVEFLRRLVLHVLPPSLHKLRHIGLYASAPR"
     gene            complement(967..1812)
                     /locus_tag="VGD55_04275"
     CDS             complement(967..1812)
                     /locus_tag="VGD55_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0869590.1"
                     /translation="MDMSIEQLGQDLARAGYAKSTQNQYVRTGGHLARRFGRPVADLT
                     SADIRIYIDEAVARKKSSSWTIMQMAAVAFLYRRTLGRPEVVSFISYPRRYLPLPTVL
                     SLEEVGSLLQAIRHPCYQAVAMVLYGAGLRITEALALEVTDIDGARGVIRVRHGKGNK
                     AREAKLSQALYEWLRRYWAEHRPVPPYLFAQPRTKKPPIPTTICKALALAAKAAWIKK
                     RVTPHVLRHSFATHLLEHGTDIRVVGALLGHASILTTARYAKVTEKLVRQTPSPLDLL
                     PHKRW"
BASE COUNT          347 a          634 c          692 g          345 t
ORIGIN      
        1 tcgtggggcg gaggcgtaga ggccgatgtg ccggagcttg tggaggctgg gcgggagtac
       61 gtgcaggacg aggcgtcgca agaactcgac gggtgcgagc gcttcgacgg cgccgttcct
      121 ggtgcggaag cggacgatgt cgtgggtgac cttgacgagc ctgctgttgg cgatgccaac
      181 gcggtgggtg tagcggccga gatagtcgag gacgtgccgc cccatcttga agggggcctt
      241 ggagtagacg ttccacgtct gcctggctgc tcgacgcatc aagcgatcaa agccgtcagg
      301 gtcgtcaaag ccggcgaagc ccgtgaaggc atgacgtcgg tcggcacgtc ggagcagggc
      361 gagcatcttc gcccggaaga cgttgcccat ggccctgacc gggaagagat agcgccgatc
      421 ggcgtcgtgc cagcgggcgc cgtcggtgga cagggctccc ccggtcacga tggcgtggat
      481 gtgcggatgg aagtggaggg cacgcgtcca ggtatgcagg acgagagtcg cgccgagagt
      541 cccgccgagc cggctctgtc caaactcgag cagagtgcgt tgcgacgctt gaaacaaggc
      601 gttatagacg atggaggggg cgaacctagc gaggggtcgc agctccgcgg gcagggtgaa
      661 gacgacgtgg aagtgcctca cgtcgagctg ggcagctcga cgttcctcga tccacttctc
      721 ttgggcgagc gccggacact tgggacaatg gcgattgcga cacgagttgt acgaggggtg
      781 ctcgtagcca cacgtgaggc accgatccac gtgacctccg agcgcggcgg tacggcactg
      841 ggcgatgtcg gtcagaacac gcttctgcgc cgtcgtgagc cgcacggagg cttcgagcgc
      901 gctgcgatga gcgcgcacga tgtccgcgat gtcgaagcgc ccggccttcg ccgggcgggg
      961 caccccttac cagcgcttgt gcgggagcag gtcgagcggg ctcggcgtct ggcgaacgag
     1021 cttctcggtc accttggcgt agcgcgccgt cgtcaggatc gaggcgtgac cgagcagcgc
     1081 cccgacgaca cgaatgtcgg tgccgtgctc gagcaagtgc gtcgcgaagc tgtgacggag
     1141 cacgtgcggg gtcacgcgct tcttgatcca agctgccttt gcggcgagtg ccagcgcctt
     1201 gcagatcgtc gtagggatcg gcggcttctt cgtcctcggc tgcgcgaaca ggtacggcgg
     1261 caccggtcga tgctcagccc agtaccgacg cagccactca tagagcgcct gcgacagctt
     1321 cgcttctcgt gccttgttcc ccttgccatg acgaacccgg atcacaccgc gggcgccatc
     1381 gatgtctgtc acctcgagcg cgagcgcctc cgtgatccgc agccccgccc cgtacagcac
     1441 catcgccacc gcttggtagc agggatgacg gatcgcctgg agcaacgacc ccacctcctc
     1501 aaggctcaga accgtgggta gggggaggta tcgtctcgga tacgagatga atgacaccac
     1561 ctccggtcgt cccagcgtcc ggcggtacag gaacgctacc gccgccatct gcatgatcgt
     1621 ccaagaggag ctcttcttcc gagccaccgc ctcgtcgatg tagatgcgaa tgtccgcgga
     1681 cgtcaggtcc gcgacgggtc gaccgaagcg tctcgcgaga tgtcctcccg tccgtacgta
     1741 ctgattctgc gtgctcttcg cgtaccctgc cctagccagg tcctgaccca gctgctcgat
     1801 gctcatgtcc atgatggcct ccttcggcga tgcctggctg ggcaacgccg actacaaggc
     1861 gaccatcaga agcggcgcag gtcaggtcct ggcgcttcag ccgtcacaac cgccgcgtag
     1921 cggcttcgtt cgaccttcct gcagcggcga tgaacctgcc tgcagcggcg atcacgggtg
     1981 cttcacgcgg gaaccaccgc tcgccagacg actactcc
//
LOCUS       DASWVF010000237         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_108548, whole genome shotgun sequence.
ACCESSION   DASWVF010000237 DASWVF010000000
VERSION     DASWVF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108548"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..628
                     /locus_tag="VGD55_04280"
     CDS             224..628
                     /locus_tag="VGD55_04280"
                     /inference="COORDINATES: protein motif:HMM:NF026923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm7 family immunity protein"
                     /protein_id="HEY0869591.1"
                     /translation="MFEWHGWASVFGSPTADDGPEADQRDRLAKQQVNDLIDTRENVN
                     NEVTDARIANGALHVWLAGSHNRRDSSPLDLFKEIARLAPGSYGVMYTVDFDTDSGWQ
                     RWVMRRGSVYAEADASLSPTIPMTEDEYPESR"
     gene            complement(745..1167)
                     /locus_tag="VGD55_04285"
     CDS             complement(745..1167)
                     /locus_tag="VGD55_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869592.1"
                     /translation="MMGGMRSGIVTRQEVQQWWSDLVAGRRSRIEASDWAEQWLGGSG
                     EELVVQGLLALQAVRHSVLSDLELRPQMADALARWQQELRLYDVDPDAWNRSYFKRLL
                     LDFARRHGAEPARKFGLKLVRSGQLREPDIEDALRSLD"
     gene            1395..1889
                     /locus_tag="VGD55_04290"
     CDS             1395..1889
                     /locus_tag="VGD55_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869593.1"
                     /translation="MFARKLALILGGLCLGLAFTSCASGGAAPQSPSASSAPGTTCTQ
                     GWSCVDQAQNRLSFVLLTPPVSTPFVSLIVFQSASHVTLLEWRDVTGGSVKLASYDTL
                     AKEEGKPVSVRGTQGYLQMLGKGSTAEAFLTWSEGGRWQLLSVPRATQQQILTLAQDL
                     TSHR"
     gene            2243..>3459
                     /locus_tag="VGD55_04295"
     CDS             2243..>3459
                     /locus_tag="VGD55_04295"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0869594.1"
                     /translation="MGRVVIGMDPHKRSATIEIIDDREKVLGQGRFSTDNAGYRAMLA
                     AGRKHSDRVWAVEGCNGIGRHIAQRLVADGEPVVDVPAKLSARARVFSTGQGRKTDPV
                     DAHSVALVGLRSSGLRRIAVDDATVALRLLVDRREDLGVARTVALNRIHRLLLELLPG
                     GAKTHLSATQAKALLAKVRPRDIVGKTRRRLAAELIVELAGIDARIKTADKELHALID
                     TTGSSLRDLNGIGPANAARLLGDIARFPDKGRFASWNGTAPLDASSGDQVRHRLSRAG
                     NRRINRVLHIMAVVQLRNDTEGRRYYDRKVAAGKTSMEAMRCLKRRLSDVVYKQMRDD
                     AQRVATGPGGHSGAANDSSAVDSNPDIDASEKSSSGPVNRNVTPTESPRLHRSSARSR
                     RPVTTPARSRRQA"
BASE COUNT          650 a         1105 c         1116 g          588 t
ORIGIN      
        1 agacgatcgg ccacggttgc gtcttccacg gacccagatt acgtccgggt cgaccgtccg
       61 gggtcttgtg tccgtactca ctgtcagcgg cagttgcgga tgccattggg ggagccgaca
      121 tcgtctcgag agaggggccg cgatccggcc gaggacacat ccaccgtgcc gtagcaatca
      181 ttggctggag gagcgtcagc gcgacgcccg ccatacttag cacatgttcg agtggcacgg
      241 ttgggcgagc gtcttcggca gccccacggc tgacgacggc ccagaagcgg accagcgcga
      301 ccgtctcgcc aagcagcaag tgaacgacct aatcgacact cgcgagaacg tcaacaacga
      361 ggtgaccgac gcccgcatag ccaacggcgc gcttcacgta tggcttgccg gctcccacaa
      421 tcgccgggat agctcaccgc tggatctgtt caaggaaatc gcccgcctgg ctcccggctc
      481 ctacggcgtg atgtacaccg tcgacttcga taccgacagc gggtggcagc gatgggtcat
      541 gcgacgcggc agcgtgtacg ccgaggctga cgccagcctg tcgccgacca tacccatgac
      601 cgaggatgaa taccccgagt ctcgctaagg ccgaggcacc cggcgccgcc ctacggcctt
      661 cccgaaatcc gccgatcggc ccgaatcaac gcaccacccg cggcagatga gtgcgtttgt
      721 cactgctcct tcgtcaagac gaactcaatc gagactacgg agcgcgtctt cgatgtccgg
      781 ctctcgcaac tgcccagatc tgaccagttt gaggccgaac ttgcgcgccg gttcggcacc
      841 atgtcggcgt gcgaagtcca ggaggagccg cttgaagtag ctccgattcc aagcgtcggg
      901 atcaacatcg tagagccgca gttcctgttg ccagcgcgcc agagcgtcgg ccatctgtgg
      961 cctcagctct agatcggaaa gcaccgaatg gcggacagcc tgaagcgcaa gaagaccctg
     1021 tacgacgagt tcctcaccgc tgccgccgag ccactgctcg gcccagtcgg atgcctcgat
     1081 gcggctgcgg cggccagcca ccaggtcgga ccaccattgc tgtacttctt ggcgcgtgac
     1141 gatccctgat cgcatgccac ccatcatccc gtcttagcca aagtcggctc gcaagaccgc
     1201 aaggtcagcg cccggatgca aaccggacgc agcacggacg gcggcagacg cggacgaagt
     1261 cgctcaaggg ggaggcgtcg tggcccgacg tcctaggtag ctcgccgggc gaccgaaatc
     1321 cgtttgccgc gtcggttgag tcgatccgct gccaacaggg cgagttcaag aacgcggcgt
     1381 ggaggggcaa gttcatgttc gcgcggaagc tggccctgat attgggtggc ctctgtttgg
     1441 gtctcgcgtt cacgtcatgc gcgagcggcg gtgcagcgcc acagtccccg tccgcatcta
     1501 gcgcgccagg caccacctgc acgcagggct ggtcttgcgt cgaccaagcg cagaaccgcc
     1561 tcagcttcgt cttgctcacg ccaccggtct ctacgccatt cgtctcgctg attgtgttcc
     1621 agtcggcgag ccacgtcacc ttgttggagt ggagggacgt taccggcggg tcggtgaaac
     1681 tggcgagcta cgacacactg gccaaagagg agggcaagcc ggtttcggtc cgcggaacgc
     1741 agggatatct tcagatgctc gggaaggggt cgaccgcgga agcgttcctc acttggagcg
     1801 aaggcggtcg gtggcagcta ctgagtgtgc cccgcgcgac ccaacagcag atcctcactt
     1861 tggctcaaga cctgacgtcc caccgctagc aacgaaacca tgctcgcaaa cgaggtgacc
     1921 ggcccgaccc caaccccggg aggcggccgg atcttgttta ctgaccggtg ccaattagca
     1981 tccgcaaatc ggcaggagcg aacgtgcgtc atcgcggcta cttgtttagt tgacgcacgg
     2041 tcggtgcctg gcaaggctcg gtcgaggtgg tcggtggggt cgttctcatc gcggctgtcc
     2101 acgtttggtc gtgtgactct catttggcat gcgtcccgcc gtcaccgtgg cgtggagagg
     2161 ctcgagaggg gtttgctcag ctcgaagtag cggtgccctc ctcgccggtt tgtatttatt
     2221 ggcgcgaggc gaggagcaca tcgtgggacg tgtcgtgatt gggatggatc cgcataagcg
     2281 gtcggcgacg atcgagatca tcgacgaccg ggagaaggtg cttggccagg gcaggttcag
     2341 caccgacaac gccggttacc gggcgatgtt ggcagcaggt cgcaaacaca gcgaccgggt
     2401 gtgggcggtg gagggctgta atgggatcgg ccgccacatc gcgcagcgac tcgtcgctga
     2461 tggtgaaccg gtggtggacg tgccggcgaa gttgtccgca cgggcgaggg tgttctccac
     2521 cgggcagggc cgcaagaccg atccggtcga cgcgcattcg gtcgcactgg tcgggttacg
     2581 ttccagcggg ctgcgccgta tcgcagttga tgatgcgacg gtcgcgttgc ggttgttggt
     2641 cgaccgccgc gaggatctcg gtgtcgcgcg gaccgtggcg ctgaaccgga tccaccgcct
     2701 gttgttggag ctgctgcccg gtggcgcgaa gacgcacttg tccgcgacgc aagcgaaagc
     2761 cttactggct aaggtccgcc cgcgtgacat cgtagggaaa acccggcgaa ggttagctgc
     2821 ggaactcatc gtcgagctgg ctgggatcga tgcgcggatc aaaaccgcgg acaaggaact
     2881 gcatgcgctg atcgacacca ccggcagtag cctgcgcgac ctgaacggca tcggcccagc
     2941 caacgccgca cgactgctcg gcgacatcgc ccggttcccc gacaaaggac gcttcgcgtc
     3001 ctggaacggc accgccccat tggatgcatc ctccggtgac caagtccggc accggctgtc
     3061 gcgtgcgggg aaccggcgga tcaaccgggt gctgcacatc atggccgtcg tgcagctacg
     3121 caacgacacc gaaggccgcc gctactacga ccggaaagtc gccgccggca agaccagcat
     3181 ggaagcgatg cgctgtttga aacggcgact gtccgacgtc gtctacaaac aaatgcgcga
     3241 cgacgcgcaa cgagtagcga cgggcccggg gggacactcg ggggcggcta atgactccag
     3301 cgcggtcgac tcaaaccccg acatcgacgc ttcggagaag tcctcgtccg gacccgtcaa
     3361 ccgaaacgtt acccccaccg aaagccctcg actacaccgt tcttcggccc gttcccgacg
     3421 ccctgtcacc acgccggccc gcagccgtcg tcaagcgca
//
LOCUS       DASWVF010000238         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_363132, whole genome shotgun sequence.
ACCESSION   DASWVF010000238 DASWVF010000000
VERSION     DASWVF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363132"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1067
                     /locus_tag="VGD55_04300"
     CDS             267..1067
                     /locus_tag="VGD55_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869595.1"
                     /translation="MSSLLLLTNALQPSSEVLPALGLLLHKVRVAPAEVAALLDAPPA
                     DAVLVDGRRDLAHARSLCQLLRTTGLNCPLFLVMTEGGLSVVTAEWGIDDVLLDSTGP
                     AEVEARLRLATGRLELAARNEDADDEVIRSGELTIDEATYSARLRGRSLDLTFKEFEL
                     LKYLAQHPGRVFTRAQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGTDHEGLIGTVRNV
                     GYKFVLPQVDRASAHSEPADDQTDDEDDLALVIRDVEMPLREMPIRAE"
     gene            1116..2057
                     /gene="mshD"
                     /locus_tag="VGD55_04305"
     CDS             1116..2057
                     /gene="mshD"
                     /locus_tag="VGD55_04305"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEY0869596.1"
                     /translation="MDNAVTRFEAAGRLTPTQIATVVALVDDATAADGVRPLSEHVML
                     HLRYGGDESTRNLLIWRGSELAAYAHLDVTDPVEGASTEMVVAPALRRQGFGRALISA
                     ALEQAGGPMRLWAHGGLAEASLMAENLGFQRVRVLFQLRRSLVAPLPAVTLPDGVTVR
                     TFQPGQDDEAWLALNARAFAGHPEQGAWTLEDLQHRIAEQWFNPAGFFLAEKVIDGAR
                     KLIGFHWTKVHGRHKHGAHDHGHEPMGEVYVVGVDPAAQGVGLGRDLTVIGLEYLRGL
                     GLQQVLLYVDESNVAAIKLYESLGFARWDTDVLFRHA"
     gene            complement(2067..2246)
                     /locus_tag="VGD55_04310"
     CDS             complement(2067..2246)
                     /locus_tag="VGD55_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869597.1"
                     /translation="MSDPARVRVEFDARMQDPTILEEIDLYSELMIVAAASSGALTTE
                     AIDRVLGLHDNRVAG"
BASE COUNT          453 a          910 c          864 g          491 t
ORIGIN      
        1 gtcgagcgtg tcggccacat aaggttcctc ggacgtgccg gcagcctcgc gagccgccgc
       61 ccagtaccgg atcacgccgg tggccggggc gtcggaaacg cggcggttac ccgcgttcat
      121 attgaggaaa ccagatggaa acagtcacac tcgtatcctt acctgaagtc cgcaggtgtc
      181 cggagagtta accggagcga cgcggcgccc tcagcgtcga ggggcatggc acccattctg
      241 gctaattccg gtgaagggcg gccgacgtga gcagcctctt gttgctgacc aatgccctac
      301 aaccctcttc tgaggttctg cccgcccttg ggcttttgct gcacaaggtc cgggtggcac
      361 ccgccgaagt ggcggcactt ctcgacgctc ctccggctga cgccgtcctc gtcgacggca
      421 gacgcgatct cgcgcacgcc cgctcgctgt gccagctcct gcgcacgacg ggactgaact
      481 gcccgctctt cctcgtcatg accgaaggcg gcttgtcggt cgtcacagcc gagtggggca
      541 tcgacgacgt gctgctcgac tcgaccggcc cggccgaggt tgaggcgcgg ctgcgtctcg
      601 cgaccggccg tctcgaactg gccgcgcgca acgaggacgc tgacgacgag gtcatccgtt
      661 ctggcgaact cacgatcgac gaggcgacct actcggctcg cctgcgtggc cgctcactcg
      721 acctcacgtt caaggagttc gagctgctga agtacctcgc gcaacaccca ggccgcgtgt
      781 ttactcgcgc ccagttgctc caggaagtct ggggttacga ctatttcggc ggcacccgga
      841 cggtcgacgt ccatgtgcgg cgccttcgcg ccaagctcgg taccgatcac gagggactca
      901 tcggaacggt tcgcaacgtg ggctacaagt tcgtgttgcc gcaggtcgac cgcgcctccg
      961 cgcactccga gcccgctgac gatcaaaccg acgacgagga cgacctcgcc ttggtcattc
     1021 gcgacgtcga gatgccgctg cgcgaaatgc cgattcgcgc ggagtaatcg ccgcgtctta
     1081 ggtccgggcg gttgcgacgc cctagggtca acggcgtgga caacgccgtc acccgtttcg
     1141 aggccgccgg acggttgacg ccgacccaga tcgccacggt cgtcgcgctg gtcgacgacg
     1201 cgactgccgc cgacggcgtc cggccattgt ctgagcacgt catgctgcat cttcgctacg
     1261 gcggcgatga atcgacccgc aacctgttga tctggcgcgg ttccgagctc gcggcgtacg
     1321 cccatctcga cgtcaccgac ccggtcgaag gcgcgagcac ggagatggtc gtagcgcccg
     1381 cgttgcgccg gcagggcttc ggccgcgcat tgatatccgc cgcactcgaa caggccggcg
     1441 gacccatgcg actgtgggca cacggcgggc tcgccgaggc gtcactcatg gccgaaaacc
     1501 ttggattcca acgggttcgg gtgctgttcc agcttcgccg gtcgctggtt gcgccgcttc
     1561 ccgccgtgac actgcctgac ggcgtgaccg ttcggacttt tcaaccaggc caggacgacg
     1621 aggcgtggct agccttgaat gcgcgcgcgt tcgccgggca tcccgaacaa ggcgcctgga
     1681 cgctcgaaga tttgcagcac cggatcgcgg agcagtggtt caatccagcc ggcttcttcc
     1741 tggccgagaa ggtcatcgac ggtgcacgaa agctcatcgg gttccactgg accaaggtgc
     1801 acgggcggca caaacacggt gcgcacgacc acggccacga gccgatgggc gaggtctacg
     1861 tcgtcggcgt cgacccggct gcacaaggtg tgggcctcgg gcgcgacctc acggtgatcg
     1921 ggcttgaata cctgcgcggg ctcggcctgc agcaggtgtt gctctacgtc gacgagtcca
     1981 acgtggcggc gatcaagctg tacgaatcac tcggcttcgc ccggtgggac accgacgtgc
     2041 tgttccggca cgcgtgagac agctgatcaa cccgcgacgc ggttgtcgtg caggccaagc
     2101 actcgatcaa tggcctcggt cgtcagcgcc ccggaactcg cggcggcgac gatcatgagt
     2161 tcgctgtaca ggtcgatctc ttccaggatc gtcggatcct gcatgcgggc atcgaactcg
     2221 actcttaccc gagccggatc gctcatgatg ctcccctatc gcacctgcgg tcggacctgc
     2281 cttgaacccc gtccgttgat tcacgctaac cggacgccca tgcgttttgc atgggcaaat
     2341 cgggtcattt gagcgcgtcg agaagaccct tgcccgtggt ttcggccttc cactcccctc
     2401 agttcgggtc gccaagttgc cgataggcga ccatgcaaat catgtacgtc gagacgtgcc
     2461 cggaatgcga ttgcgctgtc gttcgcgcgc caacgggtga gaccaagccc tcgcgccgga
     2521 tggccgcctg cacaggctgc gcctgccacg ccgagatgca aatcgcctcc agcggctgat
     2581 cggccctgtc gccacagtca catgtggcgc cgggtagggg tctatcggcc cgcccgcgac
     2641 cgcaggtcga tacaggctat ctagtcactc gtgaccgttt cgtggcctgt agtccatttg
     2701 gagtcaagga aaccccgg
//
LOCUS       DASWVF010000239         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439871, whole genome shotgun sequence.
ACCESSION   DASWVF010000239 DASWVF010000000
VERSION     DASWVF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439871"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGD55_04315"
     CDS             <1..435
                     /locus_tag="VGD55_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869598.1"
                     /translation="PMALIGGFITPAQTLPDETPPDQREAISQLETLMRARVEHLTEL
                     AIATGPAWMRPLGPAPRNPRDHTSWIHAVATIAAYRDRYTIRDDDHTVGEADFRDPGC
                     DHAYHVAVAAGRIVRSAIDHERRRHQRLYAAPTPTRGAYRDL"
     gene            471..659
                     /locus_tag="VGD55_04320"
     CDS             471..659
                     /locus_tag="VGD55_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0869599.1"
                     /translation="MDKLLFTVREAADMLGIGRSKLYELLRAGTVPSVRIGSCRRIAA
                     LDLADLVDKLRLADDAQL"
     gene            670..1821
                     /locus_tag="VGD55_04325"
     CDS             670..1821
                     /locus_tag="VGD55_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0869600.1"
                     /translation="MATRRGKRGNGEGSIYQDADGRWRSVVDLGYREGRRCRKYLSGQ
                     TRNEVATKLRRILDEHEAGLEINTNGRAPTVEAWLNHWLDNIAVQKVRPSTLVGYRGY
                     VRNRIIPTIGRHRIDKLRPEHLERFYAELIAEGLSPATIRQVHRITSRALKVAVQRGR
                     IRHNVATLVELPPVIQHEIEPLSAEQARAVLATARGRRNAARWSVALAMGLRQGEALG
                     LMWEAIDLDAGTITVRQALQRKPGGGLVMVPPKSRAGRRVIPMPAQLTEALRAQRVSQ
                     AAERLAAGPAWTDVGMVFATPFGKPVDPRNDYREWRQLLRDAGVPPARLHDARHTAAT
                     LLLTQGVPARVVMEILGHSQISLTLGTYTHVVPELARDAANRMGHALWG"
     gene            2070..2282
                     /locus_tag="VGD55_04330"
     CDS             2070..2282
                     /locus_tag="VGD55_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869601.1"
                     /translation="MTDKDRVRVALNMLRDEFEELKSLAEANGDTVSGYLRKALATER
                     YIKARTSAGGRVLVEEDGVQREIVFL"
     gene            2298..2594
                     /locus_tag="VGD55_04335"
     CDS             2298..2594
                     /locus_tag="VGD55_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869602.1"
                     /translation="MTENNASESEPEEIDLGSLRLGGDALVSVGKGKKVEDTRARLAF
                     SLLALLTAVLGLLFLLLFVHTIDAQQFTQLAGLLLAPLVGLVAAATGYYYGGER"
     gene            2591..>3133
                     /locus_tag="VGD55_04340"
     CDS             2591..>3133
                     /locus_tag="VGD55_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869603.1"
                     /translation="MTSAAESLTGITLKGGWEVLGRTDTFDDRSGGVFSVGYIVRNTS
                     GQMAFCKALDYSAAFADDVEDTEEELRRLTDAFLFERDVADRCRERKLDRVVRALDHG
                     RVRVPGHAYGWVSYLLFEKAHGDARDALDGALEVGDQYDTALRRSHDCAIALTQLHGI
                     GVSHQDVKPANLMGWKDDGGW"
BASE COUNT          616 a         1031 c          991 g          495 t
ORIGIN      
        1 ccgatggcac tcatcggtgg gttcatcacc cccgcgcaga ccctgcctga cgagaccccg
       61 cccgaccagc gggaagcgat cagccaactg gaaaccctga tgcgggcccg cgtcgaacat
      121 ctcaccgagc ttgcgatcgc aaccgggcca gcctggatgc ggccgctggg accggcgccg
      181 cgcaacccgc gagatcacac atcgtggatc cacgccgtgg caaccatcgc cgcctaccgc
      241 gaccgctaca ccatccgcga cgacgaccac accgtcggcg aagccgactt ccgcgacccc
      301 ggatgcgatc acgcatacca cgtcgccgtc gccgccggcc gcatagttcg atccgcgatc
      361 gaccacgagc gccgccgtca ccaacggcta tacgccgccc cgactcccac ccgaggcgcg
      421 taccgcgacc tgtgaacaaa cctgcgcgtc aacgatgaga aggagtcaag atggacaagc
      481 tgttgttcac cgtccgcgaa gccgccgaca tgctcggtat cggccgctcg aaactgtacg
      541 aactgctacg cgcaggcacc gtcccgtccg tacgcatcgg cagctgtagg cgcatcgccg
      601 ctcttgacct agcggacctg gtcgacaagc ttcgacttgc agacgatgcg cagctgtaag
      661 ggaggacaca tggcgactcg acgcggtaag cgtgggaacg gcgaaggttc gatctaccag
      721 gatgcggacg gacggtggcg atccgtcgtc gatctcggct accgcgaggg acgccgctgc
      781 cgcaaatatc tgtcgggcca aactcggaac gaggtcgcca cgaagctgcg gcggatcctc
      841 gacgagcacg aggccggtct cgagatcaac accaacggac gcgcaccgac cgtcgaggcc
      901 tggctcaacc actggctgga caacatcgcc gtccagaaag ttcggccaag cacgcttgtc
      961 ggctaccgcg gctatgtacg caaccggatc atcccaacca tcgggcggca tcgcatcgac
     1021 aagctgcgac ccgagcacct cgaacgtttc tacgccgagc tgatcgcgga gggactatcg
     1081 ccggcgacga tccggcaggt ccaccgcatc acctcgcggg cactgaaggt cgccgtccaa
     1141 cgcggacgga tacggcacaa cgtcgccacc ctcgtcgagc taccgccggt gatccaacac
     1201 gagatcgaac cacttagcgc cgagcaggcc cgcgctgttc tcgccaccgc gcgagggcgc
     1261 cggaacgcag cacgatggtc ggtcgcgctc gcgatgggac ttcgccaagg cgaagcgctc
     1321 ggactgatgt gggaggccat cgacctggac gccggcacca tcacagtcag gcaggcactg
     1381 cagcgaaagc cgggtggcgg cctcgtcatg gttccgccga agtcgcgtgc cggacgtcgc
     1441 gtcatcccga tgcctgcgca gctcaccgaa gcgttacgag cacaaagggt gtcgcaggcg
     1501 gctgagcggt tggccgccgg accggcgtgg accgacgtcg ggatggtctt cgcgacgccc
     1561 ttcggcaagc cggtcgaccc ccgcaacgac taccgcgagt ggcgccaact tctacgtgat
     1621 gccggcgtgc cgccagcccg cttacacgac gcacgccaca ccgccgcaac gttgctactc
     1681 acccagggcg ttccggcgcg tgtcgtcatg gagatcctcg ggcactccca aatcagcttg
     1741 actctcggca cgtataccca cgtcgtccct gaactcgccc gcgacgcagc caaccggatg
     1801 ggccacgcgc tctggggctg acacaggcgg tgcgtctgga cgtcgcgcac attgagcacg
     1861 tccggatcaa ggaacgaacc gcgctgctcg ccgaccttta cccgccgccg caaggtggcc
     1921 gacctacggg cctgatcaga ccgcagccga ctcgcatttg ggggacacgg ccgattcttc
     1981 cgatctattg gctggtgcat gtatgttgct atttaggtgc atcggatgca tactattagc
     2041 acaatacggg gccacgaaag gaggcagtca tgacggacaa ggatcgcgtg agggtcgcac
     2101 tgaacatgct tcgcgacgaa ttcgaggagc tcaagtcgct ggccgaagca aacggcgaca
     2161 cggtgtccgg gtacctacgc aaggctctgg cgaccgagcg gtatatcaag gcgcgcacga
     2221 gcgcgggcgg tcgcgttttg gttgaagaag acggcgttca gcgtgaaatc gtgtttctct
     2281 agcgaaggac gccagccatg acggagaaca acgcgagcga atccgagccc gaggaaatcg
     2341 atcttggttc tctgaggctc ggcggcgacg cactcgtgag cgtaggtaag ggcaagaagg
     2401 tcgaagacac ccgagcccgc ctcgccttct cgcttttggc gctgctaacc gccgtcctag
     2461 gcctgctgtt cctgcttctg tttgtgcaca caattgacgc gcaacagttc actcaactgg
     2521 ccgggctcct gctggcgccg ctcgtgggcc tcgtcgcggc ggcgacgggc tactactacg
     2581 ggggcgaacg atgaccagcg cggccgagtc actgaccggc atcaccttga agggcggctg
     2641 ggaagtcctg gggcgcacgg acaccttcga tgaccgctcc ggtggcgtct tttcggtcgg
     2701 atacatcgtg cggaacacca gtggccagat ggctttctgt aaagcgctgg actacagcgc
     2761 cgcgttcgcg gacgacgtcg aggacactga ggaagaactt cggcgcctaa cagacgcgtt
     2821 cttgttcgaa agagatgtcg cggatcgctg tcgcgagcgc aaactcgacc gcgtcgtccg
     2881 cgcgctggac cacggccggg tgcgagtgcc gggacacgct tacgggtggg tcagttacct
     2941 gctgtttgag aaggcccatg gggatgcccg ggacgcactc gacggggccc ttgaagtagg
     3001 ggatcagtac gacacggcgt tgaggcgctc gcatgactgt gccatcgcgc taacgcaact
     3061 ccacggcata ggcgtgagcc atcaggatgt caagccggcg aaccttatgg gatggaagga
     3121 cgacggtggc tgg
//
LOCUS       DASWVF010000240         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_108598, whole genome shotgun sequence.
ACCESSION   DASWVF010000240 DASWVF010000000
VERSION     DASWVF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108598"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..791)
                     /locus_tag="VGD55_04345"
     CDS             complement(72..791)
                     /locus_tag="VGD55_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0869604.1"
                     /translation="MDESSGVGVLDKSVAVLSALEAGPASLASLVALTGLPRPTAHRL
                     AVALERHRLVARDSEGRFVLGPRLAELAAAGGEDRLIAVAGPVLAKLRDATGESTQLY
                     RRQGDVRVCVAAAELPAGLRNTVPIGTRLPLTAGSAAQVLLAWEDAERLHRGVANAVF
                     TAQTLARVRRQGWAMSVAERQPGVASVSAPVRSVAGRVVAAVSVSGPIERLSRNPGRL
                     HARAVVAAGEAISRNLARPVP"
     gene            858..2258
                     /gene="leuC"
                     /locus_tag="VGD55_04350"
     CDS             858..2258
                     /gene="leuC"
                     /locus_tag="VGD55_04350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0869605.1"
                     /translation="MGKTLAEKVWDEHVVRHAEGEPDLVYVDLHLVHEVTSPQAFDGL
                     RLAGRTVRRPDLTIATEDHNVPTIDIDQPIADLVSRTQVDTLRRNCAEFGIRLHPMGD
                     ASQGIVHVIGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPL
                     YRPKTMAITVNGVLPAGVTSKDIILAVIAQIGTGGGQGYVIEYRGEAIRALSMEARMT
                     VCNMSIEAGARAGMIAPDETTFAYLKDRPHAPQGADWDPAVAHWRTLVTDDDATFDVD
                     VTLDAAELTPFVTWGTNPGQGAPLGSSVPDPAQIADVGERGSVERALEYMGLQAGTLL
                     RDIKVDTVFLGSCTNGRIEDLRAAAGVLRGRKVADSVRVLVVPGSMAVKARAEAEGLD
                     KIFTEAGAQWRSAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVSPLVA
                     AATAVTGHLSAPSDLD"
     gene            2289..2888
                     /gene="leuD"
                     /locus_tag="VGD55_04355"
     CDS             2289..2888
                     /gene="leuD"
                     /locus_tag="VGD55_04355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0869606.1"
                     /translation="MEKFTTHTGRALPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRADPEFVLNKPEYAGADVLVAGPDFGTGSSREHAVWALQNYGFKAVISPRFADIFR
                     GNSLKGGLLTVLIGEADVEALWKAIEADPDLEVTVDLVAREVRYAGVVLPFEIDDYTR
                     WRLMEGLDDIGLTLRHADDVGTFETTRPAWKPRTIPAAV"
     gene            3056..3280
                     /locus_tag="VGD55_04360"
                     /pseudo
     CDS             3056..3280
                     /locus_tag="VGD55_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
BASE COUNT          635 a         1111 c         1163 g          553 t
ORIGIN      
        1 atctcacgca gctcatcggg gattcgctgg aacagctcaa agcgctgacg gcggggaagt
       61 gacctgatcg ctcagggcac ggggcgagcg aggtttcggc tgatcgcctc acctgcggcg
      121 acgaccgcgc gtgcgtgcaa ccggcccgga ttgcgcgaca gccgctcgat tggacccgac
      181 accgacaccg cggcaacgac gcgaccggcg accgaccgca cgggcgccga taccgaggcg
      241 accccgggct gccgttccgc gacgctcatt gcccagccct gccgccgcac tcgcgccaag
      301 gtctgcgccg tgaagaccgc gttcgcgacg ccgcgatgca ggcgttcggc gtcctcccac
      361 gccagcagca cctgagctgc tgatccggcg gtcagcggca accgggttcc gatcggaacc
      421 gtgttgcgca ggccagccgg gagctcggcc gccgcgacgc acacgcgcac gtcgccctgg
      481 cgccggtata gctgcgtgct ctcacccgtc gcatcgcgca gcttcgcaag aacagggccg
      541 gcgaccgcga tgagacggtc ttcgccaccc gctgccgcga gctccgcgag tctgggtccg
      601 agcacgaagc gaccctcgct gtcgcgggcg acaagtcggt gccgttcgag ggcgacagca
      661 agacgatgcg cggtcggccg cgggagaccg gtgagcgcga caaggctggc cagtgacgct
      721 ggccccgcct ccagtgcgga aagtacggct actgacttgt ccagaacgcc aacaccgcta
      781 gactcgtcca tgtactgata ctgccgtctc aacatgcgag acgcaagacg cacgcaagtt
      841 aaaggtggag agagctcatg ggcaagacac tggcggagaa ggtgtgggac gagcacgtcg
      901 tgcgccacgc cgagggcgaa cccgacctgg tctacgtcga cctccacctg gttcacgagg
      961 tcacgagccc gcaggcgttc gacgggttac gactcgcggg ccgcactgtt cggcgtcccg
     1021 atctcactat tgcgaccgaa gatcacaacg tgccgacgat cgacatcgac cagccgatcg
     1081 ccgaccttgt ttcgcgcact caggtcgaca cgctgcgacg caactgtgcg gagttcggca
     1141 tcaggttgca cccgatgggt gacgcttcgc aggggatcgt gcacgtcatc gggccccagc
     1201 tcgggctgac gcagccgggc atgacgatcg tgtgcggcga ctcgcatacg tcgactcatg
     1261 gcgcattcgg cgcgcttgca ttcggcatcg gcaccagtga ggtcgagcac gttttggcga
     1321 cccagacact cccgttgtac cggccgaaga cgatggcaat cacggtcaac ggcgtacttc
     1381 cggccggcgt cacgagcaag gacatcatcc tcgctgtgat cgcgcagatc ggcaccggcg
     1441 gcggtcaggg atacgtgatc gaataccgcg gcgaggcgat tcgcgcgctc tcgatggaag
     1501 cccgcatgac cgtgtgcaac atgtcgatcg aagccggtgc gcgggcgggc atgatcgcgc
     1561 ccgacgagac cactttcgct tacttgaaag accgccctca cgcgccgcag ggcgctgatt
     1621 gggaccctgc cgtcgcgcac tggcgcacgc tcgtgaccga cgatgatgcg acgttcgatg
     1681 tcgatgtcac gctggacgcc gcggagctca cgccgttcgt tacctggggc acaaatccgg
     1741 gccagggagc gcccctggga tcgagtgttc ccgacccggc gcagattgcc gacgtaggcg
     1801 agcgaggcag tgtcgagcgg gcccttgaat acatgggatt gcaggctggc accttgctgc
     1861 gtgacatcaa ggtcgacacc gtctttctcg ggtcgtgcac caacggccgc atcgaggatc
     1921 tgcgcgccgc ggcaggagtt ctgcgcggcc gcaaggtcgc cgactccgtc agggtgctcg
     1981 tcgttccggg ttcgatggcc gtcaaagcgc gggccgaagc agagggcctc gacaagatct
     2041 tcaccgaagc gggagctcag tggcgcagtg ccggctgctc gatgtgtctt ggcatgaacc
     2101 ccgaccagct ggcgccgggc gagcggtcgg cgtcgacgtc gaaccggaat ttcgaaggcc
     2161 ggcaaggtaa gggtggccgt acccatctgg tctcgccgct ggtagcggct gcgaccgcag
     2221 tgacaggtca cctctccgcg ccgtccgatc tcgattaata gccgcagacg acaacaagga
     2281 gacgttcgat ggagaagttc acgacccaca ccggtcgggc gctgccgttg cgccgcagca
     2341 acgtcgacac cgaccagatc atccctgccg tgtatctgaa gcgcgtcacg cgtaccggct
     2401 tcgaggacgg cctgttcgct gcatggcgcg ccgaccccga gttcgtcttg aacaaacccg
     2461 aatacgcggg cgccgatgtt ctggtcgcgg gccccgactt cggcaccgga tcttcgcgtg
     2521 agcacgcggt gtgggcgctg cagaactatg gcttcaaggc ggtcatctcg ccgcggtttg
     2581 ccgacatttt caggggaaac tcgctgaagg gcggcctgct gaccgtgctg atcggcgagg
     2641 ccgatgtcga ggcgttgtgg aaggcgatcg aagccgatcc cgacctggag gtgaccgttg
     2701 atctcgtggc gcgcgaggtt cgctacgccg gcgtcgtgtt gccgttcgag atcgacgact
     2761 acacgcgctg gcgtctgatg gaagggctcg acgacatcgg attgaccctg cgccacgctg
     2821 acgatgtcgg cacgttcgag acgacgcgcc cggcttggaa gcctcgcacg atccctgcgg
     2881 cggtgtgatc aggcagcact gatgcgcgtg actggtgagg ttcgcggtcc gcatttagcc
     2941 cagattttcc gcgacacaag gaaattcttg cccccggtcg ttgttttgag gccgttcggg
     3001 gcctaggttc accccgagtc gagtgtgcac tcgacaacat cctccggagg ggcacgtgaa
     3061 caaggcggaa ctgatcgact ccatcgccgg ccgcctaggc gataacaaga aagctgcaac
     3121 cgaggcggtc gaggccgtca tcgacacgat cactcgaact gtggcgaagg gcgagaaagt
     3181 cgagcgggcg gcgcggatcg cccgtaaccc ggcgacaggt gcgcaggtca aggtcaagaa
     3241 gacgtccgtg ccgaagttcc ggcccggtac gcaactgaag gctgtcgtca gcggcgcagt
     3301 gaagcttgct gcaccggcca aggtcgccaa gccggcacct gcgaaggcag caccggcgaa
     3361 gaaggtcgtg gcacgcaagg tagctggcaa ggttgcggcg gtacgcaagg ctgcagccaa
     3421 gaaggcagct ccggcgaaga aggtcgtcgc caagaaggcc gc
//
LOCUS       DASWVF010000241         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_32016, whole genome shotgun sequence.
ACCESSION   DASWVF010000241 DASWVF010000000
VERSION     DASWVF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32016"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..484
                     /gene="rplL"
                     /locus_tag="VGD55_04365"
     CDS             92..484
                     /gene="rplL"
                     /locus_tag="VGD55_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0869607.1"
                     /translation="MAKLSTDDLIDAFKELTLLELSEFVKQFEDTFGVTAAAPVAVAA
                     AGPAGGGGAAAAEVEEQDEFDVILEEAGAQKIQVIKAVRELTSLGLKEAKDLVDGAPK
                     AVIEKGTKEAAEKAKTTLEAAGAKVTVK"
     gene            871..>2175
                     /locus_tag="VGD55_04370"
     CDS             871..>2175
                     /locus_tag="VGD55_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869608.1"
                     /translation="MAASRSTSMSGPRTSGPTYAPPRVSFAKIREPLEVPNLLALQVD
                     SFDWLLGNDVWKARVDAATKAGRDDVNLSSGLEEIFEEISPIEDFSGTMSLSFRDHRF
                     EPAKNSIEECKERDFTYSAPMFVTAEFVNNGTGEIKSQTVFMGDFPLMTDKGTFIING
                     TERVVVSQLVRSPGVYFDKAIDKTSDKDVFGCKIIPSRGAWLEFEIDKRDAVGVRVDR
                     KRKQSVTVLLKALGWDDARILDRFGQYESIRATLEKDHVHTQDEALIDIYRKLRPGEP
                     PTREAAQTLLDNLYFNPKRYDLAKVGRYKINKKLGLELPFTQGTMTEDDIVATVEYIV
                     RLHAGETEMNLNGRAVPVEEDDIDHFGNRRLRTVGELIQNQVRLGLARMERVVRERMT
                     TQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNPLAG"
BASE COUNT          435 a          723 c          663 g          354 t
ORIGIN      
        1 cgagtagtcc cgtcccgccg tccgaccagc gtttcccgtc ccacatcccg cccgcatacc
       61 gcagcgttaa cttcaggaag gacccgccac catggcgaag ctcagcaccg acgacctgat
      121 tgacgcgttc aaggagctca cgctgcttga gctgtccgag ttcgtgaagc agttcgaaga
      181 caccttcggc gtcaccgcag ccgcaccggt cgcggtcgcc gcggctggcc cggccggtgg
      241 cggcggcgcc gcggcggccg aagtcgagga gcaggacgag ttcgacgtca tcctcgaaga
      301 ggccggcgcc cagaagatcc aggtcatcaa ggctgttcgt gagctcacga gcctcggcct
      361 gaaggaggcc aaggacctcg tcgatggcgc gccgaaggcc gttatcgaaa agggcaccaa
      421 ggaggcggcc gagaaggcga agaccaccct cgaggcggcc ggcgcgaagg tcaccgtcaa
      481 gtagttggct ggtaagccag ccaaaccaag cagaagaagc ggccgccccg aaacccgggc
      541 gcggtcgctt cttttcgtgc cggatgcggc cagatgggtg attgcacgtc cacaaaccgc
      601 cgtggatgct tgacgtctcg gcgtgacagc atcatcctcg ctgtagctgt gaataacttg
      661 cacaggtcca ccagggaccg attgggccga ctcgcaaggg tcgcctcgac agcagtgtgc
      721 ccattgagtg cactgctcct ttgcgctgtc ctctgacccc ccgtgtgaga acgcgggcgg
      781 tccgtcgtgc gcacgacgaa attgcgaacg acatttcgaa cgacatgcga tcgaccttgc
      841 aacacattgc gagccctgga aggaccacac ttggccgcct cgcgcagcac ctcgatgtcc
      901 ggcccccgca cttcaggccc cacctacgca ccaccccggg tttccttcgc caaaatccgt
      961 gaacccctcg aggttccgaa cctcctagct ctccaggtcg attcgttcga ctggcttttg
     1021 ggcaacgacg tctggaaggc ccgggtcgac gctgcgacca aggccggccg cgacgacgtc
     1081 aacctgtcct caggcctcga ggagatcttc gaggagatca gcccgatcga ggacttctcc
     1141 gggacgatgt cgttgtcgtt ccgcgaccac cgcttcgagc cggccaagaa ctccatcgag
     1201 gagtgcaagg agcgcgactt cacctactcc gcgccgatgt tcgtgacggc cgagttcgtg
     1261 aacaacggca ccggtgagat caagagccag acggtcttca tgggcgactt ccccctgatg
     1321 accgacaagg gcaccttcat catcaacggc accgagcggg tcgtcgtgtc acagctcgtg
     1381 cgttcgcctg gcgtctactt cgacaaggcg atcgacaaga cctccgacaa ggacgtcttc
     1441 ggctgcaaga tcatcccgag ccgtggtgcg tggctcgagt tcgagatcga caagagggac
     1501 gccgtcggcg tgcgtgtcga ccgcaagcgc aagcagagcg tcacggtgct gttgaaggca
     1561 ctgggctggg acgacgctcg gatccttgac cgcttcggtc agtacgagtc gatccgcgcg
     1621 acccttgaga aggaccacgt ccacacccag gacgaagccc tcatcgacat ctaccgcaag
     1681 ctgcgtccgg gcgagccgcc gacccgcgag gctgcgcaga cgctgctcga caacctctat
     1741 ttcaacccca agcgctacga cctggccaag gtcggtcgct acaagatcaa caagaagctt
     1801 ggtcttgagt tgccgttcac gcagggcacg atgaccgaag acgacatcgt cgccacggtc
     1861 gagtacatcg tgcggctgca cgccggcgag accgagatga acctgaacgg tcgcgccgtc
     1921 ccggtcgagg aagacgacat cgaccacttc ggcaaccggc gcctgcgcac ggtcggcgag
     1981 ctgatccaga accaggtgcg gctcggcctt gcccgcatgg agcgcgtggt tcgcgagcgg
     2041 atgacgaccc aggacgtcga ggcgatcacg ccgcagaccc tgatcaacat tcgcccggtc
     2101 gtggcgagca tcaaggagtt cttcggaacc tcccagctca gccagttcat ggaccagacc
     2161 aacccgttgg ccggc
//
LOCUS       DASWVF010000242         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_439922, whole genome shotgun sequence.
ACCESSION   DASWVF010000242 DASWVF010000000
VERSION     DASWVF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439922"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="VGD55_04375"
     CDS             complement(<1..2151)
                     /locus_tag="VGD55_04375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0869609.1"
                     /translation="MAAAKIPDVEHLLALAVDGIGGEPRDGQLAMARAVEHAMASGEH
                     LLVQAGTGTGKSLAYLVPALRHALAFDAPVRKTSEADDEGRGTSERPDTPGRSVDRSD
                     ELAPPRGPVIVATATIALQRQLIERDLPRLIESLAPALPVPPTFAILKGRRNYLCLQR
                     LHAGPDDEQEGLFDDVGASGGSSAGGSGSGASGPTSALGRDIVRINAWANKTDTGDRD
                     ELLPGVSERAWGQVAVSSRECLGATNCPYGTDCFAESARMRAMRADVVVTNHALLAID
                     ALSEVSVLPEHDVVVIDEAHELVDRVTGVATEELTAGIVERAATRCHRLAADESARLA
                     EAATALSDVLTAIPFGRLSPLPNSLVETISLVRDAARATIGAMATTTIDVGKDADAAD
                     VEVVRRQARVAVEAVFTTAERLLADSDDDVIWAASEERRGVVLRVAPLGVADLLREAL
                     FDKRTAVMTSATLTLGGRFEPVAAGLGLGDSGWVGLDVGSPFDHGRQGILYVAKHLPP
                     PGRDGISPLVLTELAELIEAAGGRTLGLFSSMRAAVDAAQALRELLDVQILCQGEDST
                     AELVRQFAAEPTSCLFGTLSLWQGVDVPGASSQLVVVDRIPFPRPDDPLMSARQKAVA
                     DAGGNGFMAVAAAQAALLLAQGTGRLLRSRTDRGVVAVLDSRLATARYAGFLRASLPP
                     FWFTTDAVVVRSSLRALDEAAGGRRKPAVHEQPTL"
BASE COUNT          385 a          791 c          693 g          317 t
ORIGIN      
        1 gagcgtcggc tgttcgtgca cggccggctt tcggcgtccg cctgccgctt catcgagggc
       61 acgcagcgat gagcgaacga cgaccgcatc ggtcgtgaac cagaacggcg gcagcgacgc
      121 gcgcaagaat cccgcgtagc gggcggtcgc caaccgcgag tcgagcaccg cgacgacccc
      181 gcgatcggtg cgcgagcgca gcaaccgccc tgttccttgc gccagcaaca acgcagcttg
      241 cgccgcagca acggccatga atccgttgcc gccggcgtca gcgactgcct tctgccgcgc
      301 cgacatcaac ggatcgtcgg gccgcggaaa cgggatccga tcaacgacga ccaactgcga
      361 cgacgcaccc ggcacatcga cgccctgcca caacgacaac gtgccgaaca gacacgacgt
      421 cggctcggcc gcaaactgcc tgaccaactc ggctgtcgag tcttcgccct gacacagaat
      481 ctggacgtcg agcagctcac gcaatgcctg cgcagcgtcg accgctgcac gcatcgaaga
      541 gaacaatccc aacgtgcgac cgcccgcagc ctcgatgagt tcggccagtt ccgtcagcac
      601 cagcggggag attccgtcgc ggcctggcgg cggaagatgc ttcgccacat agagaattcc
      661 ttgcctaccg tgatcgaacg gcgagccgac gtcgagcccg acccacccgc tatcgcctaa
      721 accgaggccc gcggcgaccg gctcgaatcg tccgcccaac gtcaacgtcg ccgatgtcat
      781 gacggccgtt cgcttgtcga acaaagcctc gcgcaacagg tcggcaactc ccaatggcgc
      841 gacgcgcaac accacgcctc gtcgttcttc ggacgccgcc cagatcacat catcgtcgga
      901 gtcggccagc agccgctcgg cggtagtgaa caccgcctcg acagccaccc tcgcttgccg
      961 ccgcaccacc tcgacgtcgg cggcatcggc atctttgccc acgtcgatcg tcgtcgtcgc
     1021 catcgcgccg atcgtcgccc gcgccgcgtc gcgcacgagt gagatcgttt cgacgagtga
     1081 gttcgggagg ggcgacaggc ggccgaacgg gatcgctgtg agcacgtccg acaacgccgt
     1141 cgcggcttct gcgaggcgcg ccgactcgtc tgccgcgaga cgatggcaac gcgtcgccgc
     1201 gcgctcgacg atgccggccg tcaactcctc ggtcgcgacg ccggtgactc gatcgacgag
     1261 ttcgtgcgcc tcgtcgatga cgacgacatc gtgctctggc aagactgaaa cttcgctcaa
     1321 cgcatcgatc gcgagtagcg cgtgattggt taccacaaca tcggcgcgca tcgcgcgcat
     1381 gcgggcggac tcagcgaaac agtccgtgcc gtaaggacaa ttcgtcgcgc cgaggcactc
     1441 gcgcgacgaa actgcgacct gaccccacgc ccgctctgac acacctggca gcagttcgtc
     1501 gcggtcgccg gtgtcggtct tgttcgccca cgcgttgatc cgcacgatgt cacgccccag
     1561 ggcggatgtc ggccctgaag cacccgaacc cgagccgccc gcgctcgaac cgcctgaagc
     1621 gcccacgtcg tcgaacagac cttcctgctc gtcatcgggc cctgcgtgca accgctgaag
     1681 gcacaggtaa ttgcgccggc ccttgaggat cgcgaacgtc ggcggcacgg gaagcgcggg
     1741 cgcgagcgac tcgatcagtc gcggtaggtc gcgctcgatc agctgacgct gaagggcgat
     1801 ggtcgcggtg gccacgatga cgggtccgcg aggcggcgcc agctcatcgc ttcgatctac
     1861 cgatcgcccc ggcgtgtcgg gccgctcgga cgtgccgcgc ccctcgtcgt ccgcctcgct
     1921 ggtcttgcgc acaggggcat cgaaggcgag cgcgtgccga agcgcgggaa ccaggtaggc
     1981 gaggctcttg ccggtgcccg tgccggcctg gacgagcagg tgctcccccg acgccatcgc
     2041 gtgctcgacc gcccgcgcca tggcaagctg cccgtcgcgc ggctcgccac cgatgccgtc
     2101 gaccgcgagc gccaggaggt gctcgacatc gggaatcttc gcagcggcca cggctcgaag
     2161 ggtagctggc acaaccggct cgagcg
//
LOCUS       DASWVF010000243         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_57360, whole genome shotgun sequence.
ACCESSION   DASWVF010000243 DASWVF010000000
VERSION     DASWVF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57360"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGD55_04380"
     CDS             <1..244
                     /locus_tag="VGD55_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0869610.1"
                     /translation="VRLFTIASNFLDPNVYFVGGGVVETTPEFREWFISRVRASTALR
                     GEQIDVAHFAVVPDLDMAGARGAAIAARTIVIGAPD"
     gene            complement(252..1388)
                     /locus_tag="VGD55_04385"
     CDS             complement(252..1388)
                     /locus_tag="VGD55_04385"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEY0869611.1"
                     /translation="MTGEDRNLGDGTRAIHAGMPEGVDGSPLARSPVFASTYRLTGAP
                     SGPYQYGRLTNPTWTTWETALAELEGGEVVSFASGIAAVSAVLMSCLRPGDVLVMAAE
                     CYYATRRLATGLLTDIGVHVHLVPDAEVNRHLDGATLVWLESPSNPKLDVCDIAALSA
                     AAHAAGAAIAVDNTTATALGQQPLALGADYSVASDTKALTGHSDLVLGHVACRETARA
                     EAVRTWRSSTGAVPGVFEAWLAHRSLMTLDLRLARQATNALAVAELLSANALSVNGAV
                     QSVRYPWLLGDPSHDVAMRQMRRPFGLVSFTLADEAAAERFLAALRIVTVATSFGGLH
                     STAERRARWGGDDVPPGFIRFSCGGEDTLDLVADVEQALVAAGS"
BASE COUNT          254 a          527 c          483 g          247 t
ORIGIN      
        1 cgtgcgactc ttcaccatcg ccagcaactt cctcgacccc aacgtctact tcgtcggcgg
       61 cggcgtcgtc gagacgaccc cagagtttcg ggaatggttc atttcgcggg tgcgcgccag
      121 cacggctctt cgaggcgagc agatcgatgt tgcacacttc gcggtcgtgc ccgatctcga
      181 catggcgggc gcgcgcggcg ctgcgatcgc cgcgcgcacg atcgtgatcg gcgcgcccga
      241 ctaacgctcg cttagctccc cgcggcgact agtgcttgct cgacgtcggc caccaagtcg
      301 agggtgtcct cgccgccgca actaaacctg atgaatccgg gcgggacgtc gtcaccaccc
      361 catcgcgcgc gcctttccgc ggtgctgtgc agtccaccga aactcgtcgc cacggtcacg
      421 atgcgcaacg cagcaagaaa gcgctcggcc gccgcctcgt cggccaacgt gaacgagacg
      481 agcccgaacg gacgccgcat ctggcgcatc gcgacgtcgt gcgacgggtc gccgagcagc
      541 cacgggtagc gcaccgactg caccgcgccg ttcactgaca gggcgttggc cgaaagcaac
      601 tcggcgacgg caagcgcatt cgtcgcctgc cgcgccaacc gcaagtcgag cgtcatcaac
      661 gaccgatgcg cgagccacgc ctcgaacaca cctggaactg ctcccgtgct cgaccgccac
      721 gtgcgcacgg cctctgcacg tgcggtctca cggcacgcga cgtgcccgag taccagatcg
      781 ctgtggcccg tcagcgcctt ggtatcgcta gccaccgagt agtcggcacc cagcgctagc
      841 ggctgctggc cgagtgcggt tgccgtggtg ttgtcgaccg cgatcgctgc gcccgccgca
      901 tgagccgctg cgctcaatgc cgcgatgtcg cacacatcga gcttgggatt gctggggctt
      961 tctagccaca cgagcgtcgc gccgtcgaga tgacggttca cctcggcgtc cggcactaaa
     1021 tgcacgtgca cgccgatgtc agtgagcagg ccggtcgcga gacggcgggt cgcgtagtaa
     1081 cactcggcgg ccatcaccag gacgtcgcca ggtcgcaggc acgacatcag aactgctgat
     1141 acggcagcaa ttccggacgc gaacgagacg acctcgccgc cttcgagttc ggcgagtgcg
     1201 gtttcccagg ttgtccaggt cgggttggtg agccggccgt attggtaagg cccgctcggt
     1261 gcacccgtca gccggtacgt cgaggcgaag acggggctgc gggcgagcgg gctaccgtcg
     1321 acgccctcgg gcatgcctgc atgaatcgcg cgggtgccgt cgccgagatt ccgatcctca
     1381 ccagtcatgc tcgccagtgt gccgcaccgc tgcttgagca tcagtaccgt gccatccagt
     1441 tcgccagttc gcgcgtcaaa cccatcgcca actgattcga cgagagggag ccgccggtgg
     1501 tgtcacgccg a
//
LOCUS       DASWVF010000244         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_134166, whole genome shotgun sequence.
ACCESSION   DASWVF010000244 DASWVF010000000
VERSION     DASWVF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134166"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGD55_04390"
     CDS             complement(<1..295)
                     /locus_tag="VGD55_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869612.1"
                     /translation="MRPTRLVAAFALVGSCLAVGAGTQLLARADAPAPLPNGTYCQGP
                     QASQPLSGPTHVMNIVLENESSVAVDNSPDAVFEKGTLDAQCGTFSETAMHSVT"
     gene            complement(407..2062)
                     /locus_tag="VGD55_04395"
     CDS             complement(407..2062)
                     /locus_tag="VGD55_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0869613.1"
                     /translation="MASTHTPRAHLSTRRLLLGATLLVTGLVTGLGAMPTAPVALAAG
                     DGTVAPALVPALTPPATVVDRIAGSDRFATAVAASVSTFPATASASAVVLASGDSYPD
                     ALVGVTLTASLDAPLLLTSGATLPSATKNEIVRVLPAGGTVYLLGGTASVPNSIAAQL
                     ATMGYHTTRYAGADRFGTALAVAAALGNPSTVMLVDGSTFADALAAGPAAAHVHAAVL
                     LTNGSSLPAADAAYLAAHHGTVYGIGTAGHTADPAAIPVFGVGSSSSTSAAVAAKFFT
                     APTAVGIATSGSFPDALAGGAQVAHLGGPLLLNSAGAIENQVGSYLTAHAPTITLARI
                     YGGTSGISAAVSDAARSALGLSNPLANTSDLGSTAARCTYVVGYRVNVSGVVNGYGAP
                     DLFEQQTVLAVVHAAVADLSSRTGQTYRYDGTTSFVPTLTNYSHGPDDLVITVASPTS
                     TNFFASAPAGEQFGAYTRWQLDGSGSSVVAISPAMLALPSGLNDAGGASLYAIVQHKL
                     GHSVGLQHSPNPLDVMYFQANPKTPSVYSPADTALLAKSLCRS"
BASE COUNT          346 a          653 c          789 g          322 t
ORIGIN      
        1 gcgtgacgct gtgcatcgcg gtctcgctga acgttccgca ctgcgcgtcg agagtgccct
       61 tctcgaagac tgcgtcagga gagttgtcga cggcaaccga tgactcgttc tccagcacga
      121 tgttcatgac atgcgtcggc cccgatagcg gctgactcgc ttgcgggccc tggcagtagg
      181 taccgttggg cagcggtgcg ggcgcatcgg cgcgggcgag cagttgcgtg ccagcgccga
      241 ccgcgagaca gctgcccacc agggcaaacg ccgccaccag ccgtgttggt ctcatcgccc
      301 gcccctggca gatgtgcatg accgagggac gcgtgcaccc ccgcccccgg gttgtaaatc
      361 catctcaaag tagcggcgaa accgggtgct ggatacccct cgaccgctac gagcggcaga
      421 gcgatttcgc gagcagcgcg gtgtctgcag ggctatagac cgacggcgtc ttcgggttgg
      481 cctggaaata catgacgtcg agcgggttcg gcgagtgctg caggccgacc gagtgtccga
      541 gcttgtgctg gacgatcgcg tacaagctcg caccgccagc gtcgttcaga ccgcttggca
      601 acgccagcat cgcgggcgag atcgccacga cgctgcttcc tgaaccgtcg agctgccagc
      661 gggtgtaggc gccgaactgc tcgccggccg gggccgatgc gaagaagttg gtgctcgtcg
      721 gagacgcgac ggtgatgacc aggtcgtcgg gaccgtgact gtagttcgtc agcgtcggaa
      781 cgaaggacgt cgtgccgtcg tagcggtacg tctggccggt gcgtgaactg aggtctgcga
      841 cggcggcatg gacgacggcg agcacggtct gttgttcgaa gaggtccgga gctccgtagc
      901 cattaacgac accggagacg ttgacgcggt agccgaccac gtaggtgcaa cgcgcggcgg
      961 tgctgccgag gtcggacgtg ttggccagcg gattggacag gccaagggcc gaccgcgcgg
     1021 cgtccgacac cgcggcgctg atgccgctgg tgccgccgta gatccgcgcg agggtgatgg
     1081 tcggcgcgtg ggcggtgagg tagctgccga cctggttctc gatggcaccc gcagagttga
     1141 ggagcaacgg cccgccaagg tgggcgacct gcgcaccacc ggcgagagcg tcggggaagg
     1201 acccgctcgt ggcgataccg accgcggtcg gcgccgtgaa gaatttcgcg gcgacagcgg
     1261 cactcgtgct gctcgatgat ccgaccccga acaccggaat cgcagcggga tccgccgtgt
     1321 gcccagctgt tccgatgccg tacacagtgc cgtggtgcgc ggcgaggtaa gccgcgtcgg
     1381 cggctggcag gcttgagccg ttcgtcagca gcacggcagc gtgcacatgc gctgcggctg
     1441 ggcctgcggc aagcgcgtcg gcgaacgtcg agccgtcgac cagcatgacg gtgctcgggt
     1501 tgccgagcgc ggcagcgacc gcgagggcgg tgccaaagcg gtcagctccg gcgtagcgag
     1561 tcgtgtggta gcccatggtt gcgagctggg cagcgatcga gttcggcacg cttgctgtgc
     1621 cgccaagcaa gtagacagtg ccgccggcag gcagtacgcg gacaatctcg ttcttggtcg
     1681 ccgacggcaa cgtggcaccc gaggtcagga gcagcggcgc atcgaggctc gccgtgagcg
     1741 tcacgcccac gagggcgtca gggtaggagt cgccgctcgc cagaacgacg gccgacgcgc
     1801 tcgccgttgc cgggaacgtc gatacagagg cggcgacagc ggtcgcgaac cggtcgctgc
     1861 ccgcgatccg gtcgacgacg gtagcggggg gcgtgagggc gggcacgagc gcaggcgcga
     1921 cggtgccgtc gccagcagcc agcgccaccg gcgccgtcgg catggcgccc aggccggtga
     1981 ccaggccggt gaccaggagg gtggcaccga gcagcaggcg gcgcgtggac aggtgtgccc
     2041 ggggggtgtg tgtggatgcc atttgtccat gtatcggcag tccacgcctg tgatgtacct
     2101 ccggatggca
//
LOCUS       DASWVF010000245         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_82962, whole genome shotgun sequence.
ACCESSION   DASWVF010000245 DASWVF010000000
VERSION     DASWVF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82962"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..990)
                     /locus_tag="VGD55_04400"
     CDS             complement(31..990)
                     /locus_tag="VGD55_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY0869614.1"
                     /translation="MTPGDAGPAPSMIKRGTFEVGDQVQITDPKGRKHTVLLVAGKQF
                     HTHQGALEHDELIGRPEGVVVTSTGGTAYLAFRPLLSDFVLSMPRGATVVYPKDAAMI
                     IGYADVFPGARVVEAGAGSGALSCSLLRAVGETGEVHSYERREDFAAVAEKNVVRFFG
                     SAHPAWRLTVGDLVESLVEHPLDHPVDRVVLDMLAPWECLEATAGVLLPGGLICCYVA
                     TTTQLSRVVEALRTDGRFAEPAPFETMLRTWHVEGLAVRPDHRMIGHTGFLVTARRLA
                     DGVVAPVRRRRPTSTSTALEDATVPGDEASRETASPEASEQAD"
     gene            complement(987..>1562)
                     /locus_tag="VGD55_04405"
     CDS             complement(987..>1562)
                     /locus_tag="VGD55_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0869615.1"
                     /translation="TGTVGAAWAGRVIAAGTAAFGLFLVSTRRSDLQFSALWAMLIAA
                     MLWVSSTQVLRQQALRDRLPGISARALSRSSISVLADLPLAEAVRRAQEAHAQGIVIV
                     DGEGRPTGVVREAAVTAMPAPRRPWVSTGSVARHVSAAELINASWSGEELLARLQVAA
                     ASEYVVIDPAGAVYGVLAAVDVARALSGSAA"
BASE COUNT          271 a          555 c          523 g          213 t
ORIGIN      
        1 tgacggcgtc gaagtcgccg ggccggacgc ctagtcagcc tgctcggacg cctccggcga
       61 ggctgtctcg cgagaggctt cgtctccggg aacggtcgca tcttcgagcg cggtcgacgt
      121 cgaggtcgga cggcgacggc gcaccggggc gaccactccg tcggcgagcc tccgggcggt
      181 taccagaaac ccggtgtggc cgatcatccg gtgatcaggg cgcaccgcga ggccctcgac
      241 gtgccacgtg cgcagcatgg tctcgaacgg ggcaggctcg gcaaacctgc cgtcggtgcg
      301 cagggcctcg accacgcgcg acagctgcgt ggtcgtcgcg acataacagc agatcagacc
      361 acctggaagc agcacgcctg cggtcgcttc gaggcactcc cagggcgcga gcatgtcgag
      421 cacgacgcgg tcgacagggt ggtcaagcgg gtgctcgacc aacgactcga cgaggtcgcc
      481 gacggtgagc cgccatgccg ggtgcgcgga cccgaagaac cgaacgacat tcttctcagc
      541 gactgctgcg aaatcctcgc gccgctcata cgagtgcacc tcgccggtct cgccgaccgc
      601 tcgcagcaac gaacagctaa gcgccccgga acctgcccca gcctcgacga cccgcgcccc
      661 cgggaacacg tcggcataac cgatgatcat ggccgcgtcc ttcgggtaaa cgacggtcgc
      721 cccacgcggc atcgacagca caaagtcgga gagcagcggc cggaaggcca ggtaggccgt
      781 gcctccggtc gacgtcacca cgacaccctc aggccggccg atgagctcat cgtgttcgag
      841 cgcaccctgg tgagtgtgga actgcttgcc cgcgacgagc aacaccgtgt gcttgcggcc
      901 tttggggtcg gtgatctgca cctggtcgcc gacctcgaac gtcccgcgct tgatcatgct
      961 gggggccggc cccgcgtcac caggcgtcat gcggcgctac ctgagagcgc cctggcgacg
     1021 tcgacggcag ccagcacgcc atagaccgcg ccggccggat cgatcacgac gtattcagaa
     1081 gcggcggcca cctggagccg ggcaagcaac tcctcccccg accacgacgc attgatcagc
     1141 tcggcggccg agacgtggcg ggcgaccgat ccggtgctga cccacggccg tcgtggtgcc
     1201 ggcattgccg tgactgcggc ttcccgcacg accccggtgg ggcgcccttc gccatcgacg
     1261 atcacgatcc cctgggcgtg tgcctcttgg gcccgccgga ccgcctcggc aagcggcaaa
     1321 tcggccagga cggagatcga actgcgactg agagctcgag cggaaatgcc gggcagccga
     1381 tcacgcagcg cctgctgccg caggacctgt gtcgagctga cccacaacat cgccgcgatc
     1441 agcattgccc acagcgcgct gaactggagg tcagatcgac gggtgctcac gaggaacagc
     1501 ccgaaggccg ccgtgccggc cgcgatgact cggccggccc acgcggcgcc gacggttccg
     1561 gt
//
LOCUS       DASWVF010000246         5548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_337943, whole genome shotgun sequence.
ACCESSION   DASWVF010000246 DASWVF010000000
VERSION     DASWVF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337943"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGD55_04410"
     CDS             <1..255
                     /locus_tag="VGD55_04410"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY0869616.1"
                     /translation="SSRVATVEKALADIGGTLESFYFAFGDTDVYVIAELPRQEAAVA
                     LAAAVGSSGSFSKVETVVLLTPAEIDSAMHQSVSYRPPGS"
     gene            complement(266..652)
                     /locus_tag="VGD55_04415"
     CDS             complement(266..652)
                     /locus_tag="VGD55_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869617.1"
                     /translation="MDENRAQRVVDALRSRGIDARMSKEGVYQFGVRVALSDSREAVW
                     DSDDTAELEAQVMRDGMLVGFVPVIEGSADFDEAQIIDAIARTDYDQPIAHRLATAPA
                     PAAPLAPEGGVFRRFLGGFRTGGSDS"
     gene            complement(737..1861)
                     /locus_tag="VGD55_04420"
     CDS             complement(737..1861)
                     /locus_tag="VGD55_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY0869618.1"
                     /translation="MRPRPLAQLRACLATAALLAPGAVTGCSAPAATNSSPAPGTSPI
                     YSTSATQATSTTQAASKTQATSKTQAASASGKLSLLIEPEQGIEPIYALIASARKSID
                     LTMYELVDTNAELALELAAGRGVTVRVLLDVNREKSANLAAYNELTDMKVHVAWADSR
                     YAATHQKTLLIDDSVAAVMTLNMTSRYYSSTRDFAVIDRIPSDVAAIEQVFNADFAHD
                     TVVTPSATDLVWSPHQSEPALLALINAATSSLLVENEEMALKPVTAALASAAHRGVRV
                     TVVMTDQTDWHAAFATLVAAGVVVRVYAASASLYIHAKAIVADAGTSRQRAFVGSENF
                     SSASLNQNRELGLETTDPAVVGGLAKSLTSDAAGAATWRP"
     gene            complement(1974..2816)
                     /locus_tag="VGD55_04425"
     CDS             complement(1974..2816)
                     /locus_tag="VGD55_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY0869619.1"
                     /translation="MRYTALWCKAGDALMSTLRVAWALEKAALRGQLEYRVNFIVSTV
                     MGIAYQGSGFAFIYVVLHSFHTVGGWDFGDLAFLYSLRLLAHACCYLPFNQVDMLGDT
                     VREGKFDRYLVRPMNPLIQQMTNKFQINAVGDVATASALFVFAASIAHVDFSPLHIAY
                     LVLAVIGGALAEGGFILAVSSLAFRFLETWAAAALVDNIYLMFGSYPMRVFGATTAWI
                     MTWIIPVAFVAYIPASVLLDRTNGLHIAPVVAYCAPAVGFLWFFAAYQLWRFQVRHYQ
                     SSGS"
     gene            complement(2782..3633)
                     /locus_tag="VGD55_04430"
     CDS             complement(2782..3633)
                     /locus_tag="VGD55_04430"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY0869620.1"
                     /translation="MTTTIAPAGREPLSRSPWRDVPAYRQIINSRLRTTLAYRQNVLF
                     LLAILVLQIFILRKVWTALYQGHTVVDGLTLHALLVYVTLANLQNWVLQDPTVSHYMY
                     GRVRDGQVAFDLVRPVGFVPQMFAHLAGSSFATIIFGICALPLIFFAGVLGAPASRAA
                     LLLYLASFVLSYVITTLLTLMLGMIAFWATEISGITTLYWLINQFFAGALVPVSLFPG
                     PIRILADALPFQATTYTPVAIYVGRVHGADALGALGVQALWIVLLIFGSWAMWRRALH
                     RVVVQGG"
     gene            complement(3647..4660)
                     /locus_tag="VGD55_04435"
     CDS             complement(3647..4660)
                     /locus_tag="VGD55_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0869621.1"
                     /translation="MPPVIRVENLVKEFRRPKRQEGRFGAVRTLFTRQFDVVRAVDNV
                     NFAIEPGELVGYIGPNGAGKSTTIKMMTGILVPTSGTVEVGGLVPWRERERNAMQIGV
                     VFGQRSQLWWDLPLIESFTLIGKLYRVPEAKYRRNLDRFIALLDMEEFLERPVRQLSL
                     GQRMRGDLAASMLYEPRILYLDEPTIGLDIVAKENMRTFIEEMNRDSGTTIVLTTHDL
                     ADVERLCQRLILIDHGQVLYDGGVEQLKTKYAPHRILVVELEADAVQAGRRIEAADVP
                     GAEIVEQADGVARIRFVSAHTPVQHLIAALNARYPIADLSIVEPDLEGVVRQIYDERV
                     PVG"
     gene            complement(4780..5070)
                     /locus_tag="VGD55_04440"
     CDS             complement(4780..5070)
                     /locus_tag="VGD55_04440"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0869622.1"
                     /translation="MPPPILTEDEITRALVELDCWTTDAGHLRRSVKAPSFAAGIRLV
                     DAVAQVAEAMDHHPDIDIRWTTITFTCSTHSAGGVTELDVALARRIDELAST"
     gene            5119..5544
                     /locus_tag="VGD55_04445"
     CDS             5119..5544
                     /locus_tag="VGD55_04445"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY0869623.1"
                     /translation="MVGAGEPHRQVVVGAAIIDGDRVLAAQRSEPPALAGFWEFPGGK
                     VDPGETDEAALVRECREELGIDVMLGGRIGRDWPIGEYGILRVWLAGIAAGELTAKEH
                     AALRWLTVDELDDVEWIPADRAVVDRLAGMLRDRQLNQS"
BASE COUNT         1035 a         1662 c         1886 g          965 t
ORIGIN      
        1 agcagccggg tcgcgacggt ggagaaggcg ctcgccgaca tcggcggaac tctggagtcg
       61 ttctacttcg ccttcgggga cactgacgtc tacgtcatcg ccgaacttcc tcgccaagag
      121 gccgcagtcg cactggcagc agcggtgggt tcatccgggt cattcagcaa ggtggagacg
      181 gtggttctgc tgacgccggc tgagatcgac tcggcgatgc accagtcggt cagctaccgc
      241 ccgccgggtt cgtagcgggg ccggcctagc tgtcggagcc acctgtcctg aagccaccga
      301 ggaatcggcg gaagacgccg ccctcggggg cgagtggggc agcgggtgca ggtgcggtgg
      361 cgagtcgatg agcgatgggc tggtcgtagt cggtgcgcgc gatggcgtcg atgatctgag
      421 cttcgtcgaa atcggcggac ccctcgatca ccggcacgaa tccgacaagc atgccgtcgc
      481 gcatgacctg cgcctcgagt tcggccgtgt cgtcggagtc ccaaaccgct tcccgggaat
      541 ctgacagggc aacccgcacc ccgaactggt agacgccttc tttcgacatg cgcgcgtcga
      601 tgccgcgtga gcgcagcgcg tcgaccaccc gttgtgccct gttctcatcc atacctcaaa
      661 agtaccgaac caccgactcc gatgccggga gctcgaccat ttcagccggc tgcagtcggg
      721 caccgtccca ccggacctag ggccgccagg ttgcggcgcc agctgcgtcg gaggttagtg
      781 acttggccag gccaccaaca accgccggat ccgtggtctc caacccgagt tcgcggtttt
      841 ggttcagtga cgcagaggag aagttctccg agccgacgaa cgcgcgctgc cgggaagttc
      901 cggcgtccgc cacgatcgct ttggcatgga tgtacagcga cgccgaggcg gcatacaccc
      961 gcacgacaac acccgcggcg acgagcgtcg cgaacgcagc gtgccaatcg gtctgatccg
     1021 tcatgacaac cgttacccgg acgccgcggt gtgccgcact cgccagcgcc gcggtcacgg
     1081 gtttgagtgc catctcctcg ttctctacca gcaggctcga ggtcgcggca ttgatgagtg
     1141 ctaggagggc aggctcggat tgatgcgggc tccagaccaa gtctgtcgcc gatggcgtga
     1201 cgacggtgtc gtgggcgaag tcggcgttga acacctgctc gattgcggct acgtcacttg
     1261 gaatgcgatc gatgacggcg aaatcgcggg tcgaggagta gtagcggctc gtcatgttca
     1321 gcgtcatcac cgccgcaacc gaatcgtcga tgagaagagt cttctgatga gtggccgcat
     1381 atcgcgagtc ggcccacgcc acgtgcactt tcatgtcggt gagctcgttg tacgcagcca
     1441 ggttcgcgga cttctcccga tttacgtcga gcaacacccg aacggtgacc ccgcgaccgg
     1501 cggcgagttc gagcgcgagt tcggcgttgg tgtcaaccag ctcgtacatc gtgaggtcga
     1561 tcgacttgcg ggccgacgcg atcagagcgt agatcggctc gatgccctgc tcgggttcga
     1621 tcaacaggct cagcttcccg gacgccgagg ccgcttgggt cttcgaggtt gcctgggtct
     1681 tcgaggccgc ctgggtcgtc gaggttgcct gggtggccga ggtcgagtag atcggtgacg
     1741 tgcccggtgc tggagatgag ttcgtggcgg ccggcgcgct gcagccagtg accgcaccgg
     1801 gcgcgagaag cgctgccgtg gccaggcaag cccggagctg agctaggggc cgaggtcgca
     1861 tgaacccaac gtaggtgacg ccaccgaccg gaaccgctgc gagcaccgac aggtgaacgc
     1921 agcagctgcc tgtgcaggag gcgtcagctg ccggaacgtc agctgccgga gcgtcagctg
     1981 ccggaactct ggtagtgccg cacttgaaag cgccagagct ggtatgccgc gaagaaccat
     2041 aagaacccaa ctgcgggcgc gcaataggcg acgacgggtg cgatgtgcag tccgttggtg
     2101 cggtcgagca ggaccgatgc cgggatatag gcgacgaagg cgaccgggat gatccaggtc
     2161 atgatccatg ctgtcgtcgc gccgaagacg cgcatcgggt atgagccgaa catcaggtag
     2221 atgttgtcga cgagcgcggc cgctgcccag gtctccagga atcggaaggc cagtgacgag
     2281 accgcgagga tgaacccacc ctcggctagc gcgccaccga tcactgcgag gacgagatac
     2341 gcgatgtgca gcggagagaa gtcgacgtgc gcaatgctcg cggcaaaaac gaacagggct
     2401 gatgcggtgg cgacgtctcc gaccgcgttg atctgaaact tattcgtcat ctgctgtatc
     2461 agggggttca tcggccgcac gagataccgg tcgaacttcc cttctcggac cgtgtcgccg
     2521 agcatgtcga cctgattgaa cgggagataa cagcaggcgt gcgcgagcag acgcagcgag
     2581 tagaggaagg cgaggtcacc gaaatcccag ccgccgacgg tgtggaagct gtgcaggacg
     2641 acgtagatga aggcgaaccc gctgccctgg tacgcgatgc ccatgacggt cgacacgatg
     2701 aagttgacgc ggtactcgag ctgcccgcga agcgccgcct tctccaacgc ccacgccacc
     2761 cgaagcgtgc tcatcagcgc atcacccgcc ttgcaccaca acgcggtgta gcgcacgtcg
     2821 ccacatggcc catgacccga aaatcagcaa cacaatccac aaggcctgga cgccgagtgc
     2881 gccaagcgcg tccgcaccgt gaacgcggcc cacgtatatg gcaaccggcg tgtaggtcgt
     2941 cgcctgaaag ggcagcgcat cggcgaggat gcgaatcggg cctgggaaca gcgataccgg
     3001 cacgagtgcg ccggcgaaaa actggtttat cagccaatac agcgtcgtga tgccggaaat
     3061 ctcggtggcc cagaaagcga tcattcccag catcaacgta agcagcgtcg tgatcacgta
     3121 gctgagcacg aagctggcca gataaagcag cagcgcggcg cgcgaagcgg gagcgccgag
     3181 cacgccggca aagaaaatca gcgggagcgc gcagatcccg aagattatcg tcgcgaagct
     3241 cgaaccggcg aggtgggcga acatctgcgg tacgaatccg actgggcgca ccagatcgaa
     3301 cgcgacttgg ccgtcgcgga cccggccgta catgtagtga ctgaccgtcg ggtcttgtag
     3361 cacccagttc tgcagattcg cgagcgtcac gtacacgagc agcgcgtgca acgtcagccc
     3421 gtcgacaacg gtgtgaccct gatatagcgc ggtccagacc ttgcgcagga tgaagatctg
     3481 gagcacgaga atcgcgagga ggaagagcac gttctggcga tacgccagcg tcgttctcag
     3541 ccggctgttg atgatctgcc ggtatgcagg aacgtcgcgc caaggtgagc ggctcagcgg
     3601 ttcgcggcct gcgggcgcaa tcgtggtagt cacgggacta acaccgtcat ccgaccggta
     3661 ctcgttcgtc gtagatctgc cgtacgacac cttcgaggtc gggctcgaca atcgagaggt
     3721 cggcgatcgg gtaccgcgca ttcaaggcag cgatgagatg ttgcaccgga gtgtgggctg
     3781 acacgaatct gatgcgggcg accccgtcag cctgttcgac gatctctgca ccggggacgt
     3841 cggccgcctc gatccttcgg ccggcctgca cggcgtcagc ctcgagttcg accaccagga
     3901 tccgatgcgg cgcgtacttc gtcttcaact gttcgacgcc accgtcgtag agcacctgtc
     3961 cgtggtcgat gagtatcagt cgttggcaga gccgctcgac gtcggcgagg tcatgcgtcg
     4021 tcagcacaat cgtcgtcccg gaatcgcggt tcatctcttc gatgaacgtg cgcatgtttt
     4081 ccttggcgac gatgtcgaga ccgatcgtgg gttcgtccaa atagagaatg cgcggttcgt
     4141 agagcatgga cgccgcgagg tcgccgcgca ttcgttgccc gaggctcaac tggcgaacgg
     4201 ggcgctcgag gaattcttcc atgtcgagca gcgcgatgaa gcggtcgaga tttcggcggt
     4261 acttcgcctc gggcacccgg tagagcttgc cgatgagcgt gaacgattcg atgagcggta
     4321 gatcccacca gagctggctg cgctgaccga acacgacgcc gatctgcatc gcattgcgtt
     4381 cgcgttcacg ccacggcacg aggccgccga cctcgacggt gccactcgtg ggtaccaaga
     4441 tgccggtcat catcttgatc gtggtgctct tgccggcgcc gttgggtccg atgtatccga
     4501 cgagttcgcc gggctcgatc gcaaagttca cgttgtcgac ggcgcgcacg acatcgaact
     4561 ggcgggtgaa caacgtgcgg acggcgccga aacggccttc ctgcctcttc ggacgccgga
     4621 attccttcac gaggttctcg acgcggatca cgggcggcac gacgtctcct ggatgtgttc
     4681 cgggcatgcg agaggactag accttagacg tccgggtact ggtttaccgc actccagcgg
     4741 ctgagtgcga gtgagtttcg cgttcagctg acggccgggt caggtgctgg ccaactcatc
     4801 gattcggcgg gcaagagcga cgtcgagttc ggttacgcca cccgcggagt gcgtcgagca
     4861 ggtgaaggtg atcgtggtcc acctgatgtc aatgtcgggg tggtggtcca tggcctcggc
     4921 aacctgtgcg accgcatcga cgaggcggat gccggcggcg aaactcggag ctttgacgct
     4981 tcggcggagg tggcccgcgt cagtcgtcca gcagtcgagc tcgacgaggg cccgcgtgat
     5041 ctcgtcttcg gtgaggattg gtggcggcat ggcttccatc gtggcagatc ggcggggtgt
     5101 ctgtggcagg ctgtgtctgt ggtgggcgct ggggagccgc atcggcaagt ggtcgtggga
     5161 gcggcaatca tcgacggcga ccgcgtgctt gccgcgcaac gatccgagcc accagccctg
     5221 gcggggttct gggagtttcc cggcggcaag gtcgatccgg gcgagaccga cgaggcggcg
     5281 ctggtccgcg agtgccgcga ggagctcggg atcgacgtca tgctcggcgg gcgcatcggt
     5341 cgtgactggc caatcggcga gtacggcatc ttgcgggtgt ggctcgcggg catcgcggcc
     5401 ggggaattga cggcgaagga gcatgccgcc ctgcgatggc taacggtcga tgagctggac
     5461 gacgtggagt ggatcccggc ggatcgtgcg gtcgtcgacc ggctggcggg aatgcttcgc
     5521 gatcggcaac tcaaccagtc ttgaggcc
//
LOCUS       DASWVF010000247         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_261773, whole genome shotgun sequence.
ACCESSION   DASWVF010000247 DASWVF010000000
VERSION     DASWVF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261773"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGD55_04450"
     CDS             <1..291
                     /locus_tag="VGD55_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869624.1"
                     /translation="VEAVGHLGSGFAHLAESFTPLEHQLLTGLDNITAKFATWAAGTS
                     GSASFQSFLKTAEEEGPKLVGTLGDIGSALTHLVGGFSPLLPYELQTLDFIA"
     gene            334..1773
                     /locus_tag="VGD55_04455"
     CDS             334..1773
                     /locus_tag="VGD55_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869625.1"
                     /translation="MFAGFEALKVINTITGPLTTLVNIAADGAVKLLAFAGAEDAVGA
                     SASGATGGVNGLFGAVSRLASLSLPGWLAAVLGGSATVGAAALTFGPALLTSGDQARP
                     TNSRQDVNYQNWLNAHPISGITSKGDLLQAVNDSYYLQTGSYPSFTGTALSQLGNITA
                     SFGNPAEQALYTKLFGALPVPFNSINPQDVNGLPDPFNSINPQTVNSGFIPSAPPPGG
                     GQGTPSHPLPLTPSAGTVNAAAALLQLAGVNLGAALYQGVATGINPLTPLFSTLTSNL
                     KGSAQTIAIDLSNLFSTAVSAGQAATQSLGINVATGQGLPQFGTQTNVTFDANGVRHD
                     QVVSPILAQLQGALSTDKTYISDLAKLTKAGFGAGIVQQFEAAGPTSDAAIQSLLSGS
                     SGSVTSINAAFGAVNSQAAAYGQAIAQTQYLSKIVDLETQLLAATKAQDSHPAATGAA
                     VGASINGAAKTALHKIATTPNKGPFSRAQ"
     gene            1859..>2051
                     /locus_tag="VGD55_04460"
     CDS             1859..>2051
                     /locus_tag="VGD55_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869626.1"
                     /translation="MTARSHRRSIANRLLFSAAVGYVGLIVGLAIAYRWASGPILTAA
                     LVGLTGYYAMQTQRTVTEMR"
BASE COUNT          366 a          729 c          601 g          355 t
ORIGIN      
        1 gtcgaggccg tcgggcacct cggttcaggg ttcgctcacc tcgccgagtc gttcacgccg
       61 ctcgaacacc aactgctgac cggcctggac aacatcacgg cgaagttcgc gacctgggcc
      121 gccggcacct cgggctccgc atcgttccaa tcgttcctca aaaccgctga ggaggaaggg
      181 ccgaaactcg tcggcaccct cggcgacatc ggcagcgccc tgacccactt ggttggcggg
      241 ttctcgccgc tgctgcccta cgagctgcag acgctggact tcatcgcgta actcatctcc
      301 aaccaccctg cgctcgcgtc cacattggcc gacatgttcg ccgggttcga agctttgaag
      361 gtcatcaaca cgatcaccgg gccgctgacc accctcgtca acatcgccgc ggatggtgct
      421 gtcaagctgc tcgcgttcgc tggcgcggaa gacgcggtcg gagcgtcagc ttcgggagct
      481 accggcgggg tcaacggact gttcggggct gtcagccgcc tcgcatccct ctcgcttcca
      541 gggtggctgg ctgcggtcct tggcggtagc gcaacggtcg gtgcggctgc gctcacgttc
      601 gggcctgcgt tgttgacgtc gggcgatcag gcgcggccga caaattcccg ccaagacgtc
      661 aactatcaga attggctgaa cgcgcacccg atctccggta tcacgtccaa aggagacctg
      721 ctgcaggccg tcaacgattc ctactatctg cagacgggta gctatccgtc gtttaccggc
      781 acagcgcttt cccagctggg caacatcacc gctagcttcg gcaatcctgc cgagcaggcg
      841 ctttacacga agctgttcgg ggcgcttccg gtgccgttca acagcatcaa cccccaggac
      901 gtcaacgggc tgcccgaccc gttcaacagc atcaacccgc agaccgtgaa cagcggcttc
      961 atcccgtccg ccccaccgcc aggcggcggt caggggacgc catcgcaccc gctgccgttg
     1021 acgccgtctg cgggcaccgt taacgccgca gcggcgttgc tgcagctcgc aggggtgaac
     1081 ctcggcgccg cgctctacca aggcgtcgct accggtatca acccgctcac gccgctgttc
     1141 tcgaccttga cgtcgaacct gaagggctca gcgcagacca tcgcgatcga cttgtcgaac
     1201 ctgttctcca ccgccgtgtc cgccggccaa gccgcgacgc agtcactcgg gatcaacgtc
     1261 gcgaccggtc aagggttgcc gcagttcggc acccagacga acgtcacgtt cgatgcgaac
     1321 ggcgtccgcc acgaccaggt cgtgtcaccg atcctcgccc agctccaagg cgccctctcc
     1381 accgacaaga cctacatctc cgacctcgcg aagctcacca aagccggatt cggtgccggg
     1441 atagtgcagc agttcgaagc agccggcccg acctccgacg cagcgatcca gtcgctgctc
     1501 tcaggctcct caggttcggt tacttcgatc aacgccgcgt tcggagctgt caactcccaa
     1561 gccgccgctt acgggcaggc catcgcgcaa acccagtacc tgtcgaagat cgtcgacctc
     1621 gagacgcaac tcctcgccgc gaccaaggca caggactccc accctgcggc aaccggtgcc
     1681 gcggtcggtg cgtcgatcaa cggggcagcg aagaccgcgc tccacaagat cgcgacaaca
     1741 ccgaataagg ggccgttcag ccgtgcccag tgaacgacat ccctgaacgt gtagggcacc
     1801 gacccgtaga cccgcttggg ttgtcggtct gtccgactgc cgtgaaacca tcgaggttat
     1861 gaccgcgcgc tcgcaccgcc gatcgatagc caaccggctg ctgttctcag cggccgtcgg
     1921 ctacgtggga ttgatcgtgg gcctggccat cgcatatcgc tgggcttccg ggccgattct
     1981 gacggcggcc ctcgtcggcc taactggcta ctacgccatg cagacgcagc ggacggtcac
     2041 cgagatgcgg g
//
LOCUS       DASWVF010000248         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_465529, whole genome shotgun sequence.
ACCESSION   DASWVF010000248 DASWVF010000000
VERSION     DASWVF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465529"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGD55_04465"
     CDS             <1..202
                     /locus_tag="VGD55_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY0869627.1"
                     /translation="SGRYDLEVVDVYRDRAATMASNVLAAPTLIRESPLPKRRLVGDL
                     SDTARVLRVLGVNERVTASRSR"
     gene            199..>1981
                     /locus_tag="VGD55_04470"
     CDS             199..>1981
                     /locus_tag="VGD55_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0869628.1"
                     /translation="MSTADVTGANTHEHGASLDAWPAQVAYLLRELDDARSQLADSEE
                     TLRAIRHGEVDALVVAAEAGGEQVFTLSSEDRPYRNFVENMSDGAATVSADGIILYAN
                     QAFAELVSLSSNWVVGRPVLEFLNEASRARLADLLGPSNMGGSVEATLVTSSSNTVPV
                     RISTSSAIQTNGDEITCLTVSDLTSERLAEATLAHSAQHDGLTDLPNRTLLTDRLRHA
                     LERRRSESTVLALLFCDVDGFKNINDAYGHQVGDDMLRVVAARLMTAVRPGDTVARIG
                     GDEFVILCEGLEDFNDAATIAYRASAAVAAPLTSGPPDLEMTVSIGVAVAAIGDEVTA
                     DTLLRDADEAMYKAKRQGPNVVELFDEQLRAAASSSLRLLGEMRRAQGEHELRLHYQP
                     VLQLHDEAVIGVEALIRWQHPTRGLVQPDDFVPFAERSGLIVGIGAWVVHEACEQAAI
                     WAAERQLPTSQLTISVNVSGRQLARRAGLLRAVSLALDESGVEPTSLVLEVTESALMD
                     DAEAALRVLTELKTLGVQIAIDDFGTGYSSLVYLKRFPVDLLKIDRSFVAGLGRDHED
                     AAIVRSVIDLAHALGIATVAEGIETQRH"
BASE COUNT          380 a          623 c          629 g          349 t
ORIGIN      
        1 ttccggtcgc tacgacctcg aggtcgtcga cgtctaccgc gatagagcag caaccatggc
       61 gagcaacgtg ctcgccgcac caactctaat cagggagtca ccgctgccga agcgccgact
      121 cgtcggcgac ctcagcgaca cggcacgagt gttgcgcgtc ctgggcgtca atgaacgcgt
      181 aaccgcatcg cgaagccgat gagcactgca gacgtgaccg gcgcaaatac gcacgagcat
      241 ggcgcgagcc tcgacgcctg gccggcacag gtcgcttact tgctccgcga actcgacgat
      301 gcacgcagcc aactcgccga ctctgaggag accttgcgcg cgattcgtca tggcgaagtc
      361 gacgcactgg tggtcgctgc cgaggcaggc ggcgagcagg tattcacgtt gtcgagtgag
      421 gaccggccat atcgcaactt cgtcgagaat atgtccgacg gcgcggccac tgtctcagcc
      481 gacggcataa ttctctatgc caaccaggcg ttcgcagaac tggtcagctt gtcgagcaac
      541 tgggtcgtcg gtcgcccggt gcttgagttc ctcaacgagg caagcagggc gcggctggcc
      601 gacctgctcg ggccgagcaa tatgggtggc tcggtcgagg cgacgctggt caccagcagc
      661 agcaacacag tcccggtgcg aatcagcacg tcatccgcga tccagaccaa cggcgacgag
      721 atcacctgcc tgactgtcag cgacctcacg agcgaacggt tggccgaggc aaccctcgct
      781 cattctgcgc aacacgacgg acttacagac ctgcccaaca gaacactgct gacggatcgg
      841 ctccggcacg cgcttgagcg tcgaaggagt gagtcgactg tgctcgcgct gctgttctgc
      901 gacgtcgacg gcttcaagaa catcaatgat gcgtacggcc atcaggtcgg tgatgacatg
      961 ctccgcgtcg tcgcagcgcg gcttatgact gccgtgcgtc ctggggatac cgtcgcgagg
     1021 atcggtggcg acgagttcgt cattttgtgc gagggcctcg aggatttcaa cgacgccgcc
     1081 accatcgcct accgcgcgag cgccgccgtt gccgcaccac tcacaagcgg cccgccagat
     1141 ctcgagatga cggtgagcat tggcgtggcc gtcgccgcga tcggcgacga ggtcactgcg
     1201 gacacattgc tgcgcgacgc tgatgaagct atgtacaagg caaagcgcca agggcccaac
     1261 gtggtggagc tgttcgatga gcaactaagg gcagccgctt cttcaagcct gcgtttactt
     1321 ggagaaatgc gccgcgcgca aggcgaacac gaactgcggc tgcattacca acctgtcttg
     1381 caactacacg atgaggccgt catcggggtc gaggctctga tccgctggca gcacccgacc
     1441 cgcggattgg tgcaacccga tgacttcgtg ccttttgcag aacgcagcgg cttgatcgtc
     1501 ggcatcgggg cgtgggtcgt gcatgaggcg tgcgagcaag cggccatctg ggccgcggaa
     1561 cgccaactgc ccacttcgca gctgacaatc tcggtaaacg tctcggggcg gcagcttgcc
     1621 agacgggccg ggttgcttcg ggctgtgtcg ctcgctctcg acgaatcggg cgtcgagccg
     1681 acctcacttg tacttgaggt caccgagagc gcgctgatgg acgacgccga ggcggcgttg
     1741 cgcgtgctga cagaactgaa gacactcggc gtccagatcg ctatcgacga cttcggtacg
     1801 ggctactcgt cgctggtcta cctgaagcgg ttcccggtcg acctgctcaa gatcgatcgt
     1861 tcgtttgtgg caggtctcgg gcgcgaccac gaggacgcgg ccatcgttcg cagcgtgatc
     1921 gacctcgcac acgcgctcgg catcgcaacg gtcgctgagg gcatcgagac ccagcgtcat
     1981 c
//
LOCUS       DASWVF010000249         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_363374, whole genome shotgun sequence.
ACCESSION   DASWVF010000249 DASWVF010000000
VERSION     DASWVF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363374"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..579)
                     /locus_tag="VGD55_04475"
     CDS             complement(25..579)
                     /locus_tag="VGD55_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0869629.1"
                     /translation="MSPQPTLTSPLRSADFVRDLAPFEHPGLRRLSAIPGAAVPAPEA
                     FDTLVISPRPLVERTVPLRLVDLSAPLRAERLRTSETLTPGHIRLTRRGRLALLGVAM
                     LCMLFGFSIGNLVSFGSSAPSYPTTPSAGSPTIVVQPGQTLWAIATQVAPHADPRATV
                     QRIIDLNHLGGTTLQVGESLALPS"
     gene            763..1512
                     /gene="lexA"
                     /locus_tag="VGD55_04480"
     CDS             763..1512
                     /gene="lexA"
                     /locus_tag="VGD55_04480"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0869630.1"
                     /translation="MPTDKDAAPKTPLAAVSDFPDGPPDETGLTPRQHRVLDVIRDSV
                     ERRGYPPSMREIGEQVGLTSTSSVAHQLATLEKKGYLRRDPNRPRAVEVRIPGQIAGT
                     STSEPRGTRSEPFDETESGDARPAPAYIPVVGRIAAGGPILADQVVEDVFPLPRQVVG
                     DGTLFMLNVVGDSMVDAAICDGDWVVVHQQPAAENGDIVAAMIDGEATVKTFKRRDGH
                     VWLMPHNPNYAPIPGDDATILGRVTAVLRRV"
     gene            complement(1537..>2229)
                     /locus_tag="VGD55_04485"
     CDS             complement(1537..>2229)
                     /locus_tag="VGD55_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0869631.1"
                     /translation="GLPVARPLECITYEGQCALISERIVGLDLSRVLSRAPWKVREAA
                     RVLAGAQAAMHAVVAPAELPQMHDIVEARIRSGTSTPPEVLDSALAALHGLPHGEQLC
                     HGNLHLGNVIVRDAAAVIIDCGDAARGDPWSEVAQTLVRYRCARLRPGAPLTARLGSA
                     VGRRLLGVAYLRAYLRAYLRADPGQIRRARAARAPAVLKKWEGVRAVERLAEGHPQER
                     RRLLRLARRRLS"
BASE COUNT          391 a          726 c          793 g          319 t
ORIGIN      
        1 gggcgggctg ggacgtgcgg gcggtcatga cgggagggcg agggactcgc cgacctggag
       61 ggtggtgccg ccgaggtggt taaggtcgat gatccgctgc acagtcgccc gtggatcggc
      121 gtgcggagcg acctgggtgg cgatcgccca caacgtctga ccaggctgga cgacgatggt
      181 cggggagcca gccgaaggcg tcgtcgggta gctcggagcc gacgagccga acgacaccag
      241 gttgccgatc gagaagccga acagcatgca caacatcgcg acgccgagca acgccagccg
      301 gccgcggcga gtgagtcgaa tatggcctgg cgtaagggtt tctgaggttc gcaatcgctc
      361 ggcgcgcaac ggagccgaca ggtcaacgag acgcagcggc accgtgcgct cgaccagcgg
      421 tcgcggactg atcaccaacg tgtcgaacgc ttcgggcgcg ggaaccgctg cgcccggaat
      481 ggcgctgagt cggcgcagcc ctgggtgctc gaacggcgca aggtcgcgga cgaagtcggc
      541 cgaccgtagt gggctggtga gggtgggctg cgggctcatc atgacctccg gggaaggggt
      601 atcagttgct cgagatcgaa cggatgttcg attcgaacgc ctgtacgaag ttgtaccgca
      661 tgccgccgac aaaatcacga cacgttcgaa cagatgtttg aatgcgtgtc gagaaagggc
      721 taccgtcggg caaccgaaga cggatcaggg agcgtgctcg tggtgccaac ggataaagat
      781 gcggccccaa aaacgccctt ggcggcggtc agcgacttcc ccgacggtcc gcctgacgag
      841 accggcctga cgccccgcca gcaccgggtg ctcgacgtga tccgcgactc ggtcgagcgg
      901 cgtggctatc cgccgagcat gcgtgagatc ggcgagcagg tcgggctgac gtcaacgtcg
      961 agcgtcgccc accagctggc cacgcttgag aagaagggct acctgcgccg cgacccgaac
     1021 cgtcctcgtg cggtcgaggt acggatacct ggccagattg ccggcacctc gacgtccgag
     1081 cctcgtggaa cccgtagcga gccgttcgac gagaccgaga gcggcgacgc acggccggcg
     1141 cccgcctaca ttccggtcgt cggccgcatc gcagccgggg ggccgatcct tgccgaccag
     1201 gtcgtcgagg acgtgttccc gctcccccgc caggtcgtcg gcgacggcac gctgttcatg
     1261 ctcaacgtcg tcggcgactc gatggtcgac gcggcgatct gcgacggcga ctgggtggtc
     1321 gtgcatcagc agccggccgc cgagaacggc gacatcgtgg ccgcgatgat cgacggcgag
     1381 gcgaccgtga agaccttcaa gcgacgcgac gggcacgtct ggctgatgcc gcacaacccc
     1441 aactacgccc cgatccccgg cgacgatgcg acgatcctcg gccgcgtgac cgcggtcctg
     1501 cgccgcgtct aggagatcgc gtctaggaga tcgcgtctag gagagccggc ggcgggctag
     1561 gcggaggagt cgtcgccgct cctgtgggtg tccttcggcg agccgctcga cggctcgcac
     1621 gccttcccac tttttcagga cggcgggtgc gcgggcggcg cgtgcgcgtc tgatctgccc
     1681 cgggtcggcc cgtagatagg cccgtagata ggcccgtaga taggcgacgc caagaagacg
     1741 ccggccgacc gccgaaccga ggcgtgcggt caggggcgcg cccggccgca accgggcaca
     1801 tcggtagcgc accaaagtct gcgcgacctc tgaccaagga tcaccacgag ctgcatcgcc
     1861 gcagtcgatg atgacggcgg ctgcatcgcg cacgatgacg ttacccaggt gcaggttgcc
     1921 gtgacataac tgctcgccgt gcggcagccc gtgcaacgcc gcgagcgccg agtcgagcac
     1981 ctcgggcgga gtcgacgtcc cgctgcgaat cctggcctcg acgatgtcgt gcatctgcgg
     2041 cagctcggcg ggtgcaacga cggcgtgcat cgcggcttgc gcgcccgcga gcacgcgtgc
     2101 cgcctcgcga accttccacg gcgcccgcga cagcacccgg ctgaggtcca accccacgat
     2161 gcgctcagag atcagcgcgc actggccctc atatgtgatg cattcaagag ggcgcgctac
     2221 ggggagccc
//
LOCUS       DASWVF010000250         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_108822, whole genome shotgun sequence.
ACCESSION   DASWVF010000250 DASWVF010000000
VERSION     DASWVF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108822"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1620
                     /locus_tag="VGD55_04490"
     CDS             <1..>1620
                     /locus_tag="VGD55_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0869632.1"
                     /translation="ERHGMLVIGRDAHAIRVATCDPTNVVALDDLRLYTGATTVTVVV
                     CTDSQIQTELRRVWAILDQDAELSNIADESEAAADEHDLESAADQGPTVRLATSIVTE
                     AVRSRASDIHVEPQIDGLRIRYRIDGLLRDIMKVPRNSSAALVSRLKIVSGLDIAERR
                     LPQDGRTRLTVDGSAVDARVSTLPSVHGEKVVIRLLASGDHVPSVTQLGLDETQLEAL
                     LAGTANPQGLVLITGPTGSGKTHTLYSVLSHMATPDKNVVTLEDPVEIQLPGITQVQT
                     NERAGLSFSKGLRSILRQDPDVVLVGEVRDSETAELALQASMTGHLVLTTLHTNDAVA
                     ALTRLVDMGIQPFLIASSLSLVVAQRLVRRPCADCIAPYTPSTRVLTLLGLTEADLVG
                     ATPMRGRGCGECGGTGYRGRTGIFEVLPVTASVRSVLSHTPTEAAVGAAARAAGMTTL
                     RGSGLARARRGETTFEEVLRVSQVDVTEGHRCTACDRAVEADMIACPWCATIVDRGHC
                     SSCARPLEAGWKICPWCRTAAAVPAAAIDTSV"
BASE COUNT          277 a          573 c          505 g          265 t
ORIGIN      
        1 cgaacgccat ggcatgttgg tgatcggtcg cgacgcacac gcaatccggg ttgccacttg
       61 cgatccgacc aacgtcgtcg cactcgacga tttgcggctc tacaccgggg cgacgaccgt
      121 caccgtcgtg gtgtgcaccg actcccaaat ccagaccgag ctccgccgag tgtgggcaat
      181 cctcgatcag gacgccgaac tgtcgaacat cgccgacgaa tccgaggccg ccgccgacga
      241 gcacgacctc gagagcgctg ccgaccaagg accgaccgtc cggctcgcca cgagcatcgt
      301 caccgaagcg gtgcgcagcc gtgccagcga catccacgtc gagccgcaaa tcgacgggct
      361 gcgcatcagg taccgcatcg acggactcct tcgcgacatc atgaaggtgc cccgcaactc
      421 ctcggctgca ctggtcagcc gcttgaagat cgtctccggt ctcgacattg ccgagcggcg
      481 ccttccgcaa gacggccgca cccgactcac cgtcgacggc agcgccgttg acgcgcgcgt
      541 cagcacgctg ccttcggtgc acggcgagaa ggtcgtcatc cggctcctcg ccagtggcga
      601 ccacgtgcct tcggtgaccc aactgggcct tgatgaaacc cagctcgagg cgttgctcgc
      661 cggtaccgcg aacccgcaag gactcgtgct gattacgggc ccgaccggct cgggcaagac
      721 ccacacgctc tactcggtgc tgtcgcacat ggccaccccc gacaagaacg tcgtcaccct
      781 cgaagacccg gtcgagatcc agcttcctgg tataacgcag gtgcagacga acgagcgggc
      841 cggcctgtcg ttcagcaagg gtctgcgatc catccttcgt caggaccccg acgtcgtgct
      901 cgtcggcgag gtgcgtgact cggagactgc cgaactcgct cttcaggcct cgatgacggg
      961 ccacctcgtg ctgacgactc tgcataccaa cgacgcagtg gctgcgttga ctcgactcgt
     1021 cgacatgggc atccagccct tcctgatcgc gtcgtcgttg agcctggtcg tcgcgcagcg
     1081 gcttgtccgg cgtccgtgtg ccgactgcat cgcgccctac acgccgagca cgcgggtact
     1141 caccttgcta ggactcaccg aagccgatct cgtcggcgca acaccgatgc gcggccgcgg
     1201 ttgcggcgag tgtggcggca ccggctatcg cggccgaacc ggcatcttcg aagtcttgcc
     1261 ggtgaccgcg agcgtgcgct cagtgctgtc tcacacgccc accgaagcgg ccgtcggcgc
     1321 tgcagcgcgg gcggctggca tgacgacatt gcgcgggtcg ggccttgctc gcgcccgacg
     1381 tggagaaacg acgttcgaag aagtgctgcg cgtctcccag gtcgacgtca ccgaaggcca
     1441 ccgatgcacg gcatgcgacc gtgcagtcga ggccgacatg attgcctgcc cttggtgcgc
     1501 gacgatcgtc gaccgtgggc actgcagttc gtgtgcgcgg ccgctcgaag ccggctggaa
     1561 gatctgcccg tggtgccgca ccgccgcagc tgttcccgca gcggccatcg acaccagcgt
//
LOCUS       DASWVF010000251         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_465546, whole genome shotgun sequence.
ACCESSION   DASWVF010000251 DASWVF010000000
VERSION     DASWVF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465546"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VGD55_04495"
     CDS             complement(<1..403)
                     /locus_tag="VGD55_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869633.1"
                     /translation="MRLLRRRAWALPGVLLASAVASVAIAVVWSVLLPDRVRGPVFFG
                     LDVPDGSTAAVSAATASSECAPTVQSAFVKLDSSSFTLGKLDSIATLGRPFITLEPWS
                     LVSHQNQSNQPRYSLATIASGTYDDDLRRIAG"
     gene            complement(405..>1745)
                     /locus_tag="VGD55_04500"
     CDS             complement(405..>1745)
                     /locus_tag="VGD55_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869634.1"
                     /translation="LIAHVAVWVGPWLVLAAVAGVFRLRRRFLLVMVLLTASIVGPAE
                     QVRIGEGTSLSKHVAFGLIFACPLIGDLAARTLSHWWALTIWPVLAVFGALLLSGMHY
                     STHLYGEWSNEATLSTLLRTDIASVGKQPILGERPSSQRYELQDVTYGSQWYDTYYFS
                     YDGKHDTAAYEAALDAHYFGLVYVSDTTPYGKFVQDWFARQPWQSAYYHLQAKLPRYL
                     FGHQIGWWLVYVPDPHVATTMTAGNALPSTAAYGDSVRWSGSLSADRGPTAARYPNLP
                     AEAPIVELWFNSALTGQWQNVASQHAVPAGCALCWTWSFGLPQTRSGQWQVRFLARTP
                     TILKASSGPVEAVTITPAVTGWISGSATVPWGTTIVRTITVQPGFERQAMLFERTCGA
                     CAWTRVGSPQPLDGQDQTTFVFTVLWGTRDWELYLPAIASSSDYTSDVFTTTSH"
BASE COUNT          347 a          574 c          530 g          294 t
ORIGIN      
        1 cgccggcaat gcgacgaagg tcgtcgtcgt aggtgccgct agcgatcgtc gcgagcgaat
       61 accgcggctg attcgattga ttctggtgcg acacgagcga ccaaggctcg agggtgatga
      121 acggcctacc gagcgttgca atgctgtcta gtttcccaag cgtgaacgac gaactgtcga
      181 gcttgacgaa cgcgctctgg accgtcggcg cacactcact gctcgccgtt gcagcgctca
      241 ccgcagcggt acttccgtcg gggacgtcga gcccgaagaa caccggcccg cgcacccggt
      301 ccgggagcag cacactccac accactgcga tcgcgacgct cgcgaccgcc gaggccagca
      361 gcacgccggg caatgcccac gcgcgccttc tcaggagccg cacatcagtg actcgtggtc
      421 gtgaatacgt cgctcgtata gtccgaactc gaggcgatcg ccggcaggta caactcccaa
      481 tcacgcgtcc cccacagcac ggtgaacaca aacgtcgtct ggtcctgacc gtccagtggt
      541 tgagggctcc ctacccgcgt ccaggcgcag gcgccgcatg ttcgctcgaa caacatcgct
      601 tgccgctcga accccggttg aaccgtgatc gtcctcacga ttgtcgtgcc ccacggcaca
      661 gtggcagagc cactgatcca cccagtaacc gcgggcgtga tcgtcacagc ctcaaccggc
      721 ccgctcgacg ctttcaggat tgttggcgtt cgagcgagaa acctcacctg ccattggccg
      781 gatcttgtct gcggcagccc aaacgaccag gtccagcaca gggcacaacc agccgggacg
      841 gcgtgttgtg acgccacgtt ttgccactga ccggtcagtg cggagttgaa ccacagttcg
      901 acgatcggag cctcggccgg caggttggga tagcgggctg ccgtcggacc gcgatcggcg
      961 ctgagtgacc cgctccacct cacggagtcg ccgtatgcag ccgtgctcgg cagcgcgttg
     1021 ccggcagtca tcgtcgttgc gacatgcggg tcgggcacgt aaaccagcca ccaaccgatc
     1081 tgatgaccga agaggtagcg cggcagcttg gcctgtaagt ggtagtaggc gctctgccac
     1141 ggttgccgcg cgaaccagtc ctggacgaac tttccgtacg gagttgtgtc gctgacatac
     1201 acgagtccaa agtagtgcgc gtcaagtgcg gcctcgtacg cggcagtgtc gtgcttgccg
     1261 tcgtagctga agtaatacgt gtcgtaccac tgcgagccat aggtgacatc ttgaagctcg
     1321 tatcgttgcg acgacggtcg ttctccgagg atcggttgct tcccgaccga tgcgatgtcg
     1381 gtgcggagca gcgtcgacaa cgtcgcctcg ttcgaccact cgccgtacag gtgagtcgaa
     1441 tagtgcattc ccgaaagcag tagcgcaccg aacactgcca gcaccggcca gatcgtgagg
     1501 gcccaccaat gcgacagcgt gcgcgccgcg agatctccga taagtgggca cgcaaagatt
     1561 agtccgaacg cgacgtgctt cgacagcgac gtgccctccc cgatcctgac ctgttcggcg
     1621 ggcccaacaa ttgacgccgt cagcaagacc atgacgagca agaaccgtct ccggagacgg
     1681 aagacgcccg caacggcggc gagaaccagc cacggaccca cccacaccgc gacgtgcgcg
     1741 atcag
//
LOCUS       DASWVF010000252         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_159628, whole genome shotgun sequence.
ACCESSION   DASWVF010000252 DASWVF010000000
VERSION     DASWVF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159628"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="VGD55_04505"
     CDS             complement(<1..1763)
                     /locus_tag="VGD55_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869635.1"
                     /translation="MTSLPAGCRALGTPAIRVGVLALALLFGSPVAALAASPSPNPST
                     SGSGSASKGTPPSTGSTKAVYGLGPSSATGLDRRPRFKWSASPGGTLQDHVAVVNSGA
                     TPLTLDLYARDAVNQPDGSLGLQTKAAKPTDAGAWITLEIPGGASTITVPARSTVIVP
                     LSLAVPRNASPGDHTGGVIVSLTAESTSTGKQVVAPNLEQRVAVPVAIRVSGPLQPKL
                     SVQSLHAAYGQTLNPVGGGHATVTYKVVNTGNVNLGGHEAVSISGLFGSAVHAKAQDV
                     PVLLPGNSIVESVPVHGVLPEFLMSARVTITPEAASGDVDPGLIATSGSKHFWAVPWL
                     LLGIIALLLLGWRQRRRRKRPLAGRHGTNSGGGTRRRGSSLSPSARRPVEVTHDGVSV
                     ASSFSRVAGRFAIVVAAAGLTFAIPAGTAFATSLPYSDTSAQGGIGLCDKNGINITHG
                     SVYDKPFVWRAMGATPAPASVRGSGRNATLYAYQPRKGVDPAEWSGEQLTANAAYSDP
                     NIPIAQATDRDIALSDYLNDYPPQWNRLIELRIFYGATSGDISATYPVANIQVTGTNW
                     TLVSGAKVDCKAGSAISMEDAL"
     gene            complement(1876..3729)
                     /locus_tag="VGD55_04510"
     CDS             complement(1876..3729)
                     /locus_tag="VGD55_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869636.1"
                     /translation="MKLHPKRAVAAVGAVAIAAGVTLATAGTAFAAAPYNPDSGSLGS
                     ITFTDANGVPVTSGSTTVPIAAYAAGSKLINAGDTDASLNVYLPAFGQPTALWSGEAV
                     GLDTVFSPAPAAYPTDLKGLVTAGKPVINERSTDLSPASFAADFPNTSTTGPNTAQPT
                     WQNLYQLRLFTGSTTATYDSADILISGTTWTLVPNPDQTVATTTTISASPASPISSSN
                     PLPVTLTTTVTPAGSVASGYIGGIGTVVVKDGATVIDTKIVAPGSSAPFTVTTGPINE
                     TNPSNHSFTATFTPFNGTGLLASASTALVYNISVPLPTATTTLAATWQPFAGGSTPTH
                     FSGTVTGNGTTPPPVTAGTVNLFDNGSSTPLNPTPLVVGAGGAFSSDIIIATSGPHSV
                     VATFTDAGVYLPSSSSPVTQTLGADPGALCTSSPATGAGSCTDQQTITGTVPAGTLTV
                     STPYTTAHPFNVGTLALDTSGTFLTTHAVFGGAGLVASDPNADIIVTDTRAGDLNWTL
                     QAQSSNLTDATADIINSQNVGLTGLGSHPAPGFNTAGLVGFNNAAATGVAPAAAGNLG
                     LGGAIKHDVAQATAGIGTVGIYGTLTLNAPTSTTAGIYVGTITFTIVGSLV"
     gene            complement(3908..>4555)
                     /locus_tag="VGD55_04515"
     CDS             complement(3908..>4555)
                     /locus_tag="VGD55_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869637.1"
                     /translation="DPSLGGSNAVALSGVQLAKIYQCLPGFTHWNDPGIGGTSSDSIV
                     PIIPQSGSGTRNDFLADLNAHYGLPTSTGPLGSCVQTAQEHDPDGIVLNPNAIDAIEP
                     FSAARIALINSGYFANAGVAANKVVASSGTPTDTNPIYQLTRNMYITVRDIDVNNGTV
                     WEPGGANNWAKTLFVGSSSYLSRATGQADVTAAGFVGGYVDCGAADGAATCPAFS"
BASE COUNT          806 a         1387 c         1582 g          780 t
ORIGIN      
        1 agcgcgtcct ccatggagat cgcgcttccc gccttgcagt cgaccttggc gccactcacg
       61 agggtccagt tcgtgccggt gacttggata ttcgcgactg gatacgtggc cgatatgtca
      121 ccgctcgtcg cgccgtagaa gatgcgtagc tcgatgagcc tgttccactg tggtggatag
      181 tcgttgaggt agtccgacaa cgcaatgtcg cgatcggtcg cctgcgctat cgggatattg
      241 ggatccgaat acgccgcgtt ggccgtcaac tgctcgccac tccattccgc tgggtcgaca
      301 cccttgcgcg gctggtaggc atacagcgtg gcgttgcgac cgctacctcg caccgaagca
      361 ggcgccggcg tggcacccat cgcccgccag acgaacggct tgtcgtagac gctgccgtgg
      421 gtgatgttga tgccgttctt gtcgcacagg ccgatcccgc cctgggcgga ggtgtcgctg
      481 tacggcaggc tcgtcgcgaa ggcagtaccc gccgggattg cgaacgtcaa cccggcggca
      541 gccacaacaa tcgcgaagcg gccggcgact cgcgagaaac tgctcgccac gctgacaccg
      601 tcgtgagtga cctcgaccgg cctccgcgca gacggcgaca gcgacgaacc tcgccggcgc
      661 gtgccgccgc ccgagttcgt gccgtgccga ccggccaacg gacgcttgcg ccgtctccgc
      721 tgccgccaac ccaagagcag cagggcaatg atgccgagca acagccaggg cacggcccag
      781 aaatgcttgc tgccactcgt cgcgatcagc ccggggtcga catcgcctga ggctgcctcc
      841 ggagtgatag tgacgcgcgc cgacatgagg aactccggca gcacgccgtg gactggaacg
      901 ctttccacga tcgagttccc tggcagcaga accggaacgt cttgggcttt cgcgtgaacg
      961 gccgacccga acaaaccgct gatcgaaaca gcttcatggc cgccgagatt cacgttcccc
     1021 gtgttgacca ccttgtacgt gacggtggca tgtccgccac cgacaggatt cagagtctgg
     1081 ccatacgctg catgcagtga ctgcacggaa agcttcggct gaagcggccc ggagactcgg
     1141 atcgcaacgg ggacggcaac ccgctgctcg aggttgggcg caacgacctg cttgcccgtg
     1201 gacgtcgact ccgccgtcag tgagacgatg acgccgcccg tgtggtcgcc tggcgaagcg
     1261 ttgcgcggta cggcgagcga cagcggcacg atcacggtcg agcgagctgg cacggtgatc
     1321 gttgacgcgc cgcccggtat ctcgagtgtg atccatgcgc cggcgtcggt aggcttcgcg
     1381 gccttggtct gcagtccgag gctgccgtca ggctggttga ccgcatcgcg cgcgtagagg
     1441 tccagcgtga gcggcgtggc cccgctattt acgaccgcga cgtgatcttg gagcgtcccg
     1501 ccaggactcg ccgaccactt gaaacgtgga cgtcggtcaa ggccggtggc actcgacggc
     1561 cctaacccat agaccgcttt ggtcgagccg gtcgacggtg gtgtgccctt agaggcagag
     1621 cctgatccgg acgtcgacgg gttgggtgaa ggagaggcag caagcgctgc gacgggcgac
     1681 ccgaacagga gcgcgagagc aagcactccg acgcggattg cgggggtccc gagggcgcgg
     1741 cagccggccg gtagcgaagt cactatgcga tccaagcgtc gggaactgta caaggggtga
     1801 gatcgaggtg aacgccgaac gacgaagact gggcgggcag cagagctgcc cgcccagtcc
     1861 ttagtgcggt ggggattaga cgagcgaacc cacgatggtg aaggtgatgg tgccgacgta
     1921 gataccggcc gtggtcgagg tcggggcgtt caacgtgagc gtcccgtaga tcccaacagt
     1981 gccgatacca gccgtggcct gagcgacatc gtgcttgatc gcaccaccca aaccaaggtt
     2041 gcccgcagca gccggcgcga caccggtagc agccgcgttg ttgaacccga ccagacccgc
     2101 ggtgttaaac ccaggagccg ggtggctgcc aagaccggtc agaccaacgt tttggctgtt
     2161 gatgatgtca gccgttgcat ccgtcaggtt cgacgactgc gcctgcaacg tccagttcaa
     2221 gtcaccggcg cgcgtgtcgg tcacgatgat gtccgcgttc gggtcagagg caaccaggcc
     2281 ggccccacca aacactgcgt gcgtggtcag gaacgtgccc gaggtgtcca acgccaacgt
     2341 gccgacgttg aacggatgcg cggtggtgta cggagtactc accgtcaacg tgcccgccgg
     2401 aaccgtaccc gtgatcgtct gctggtcggt gcaagaccca gcacccgtcg ccgggctgga
     2461 ggtgcacagt gcgcccgggt cggcacccaa ggtctgggtg accggcgacg agctggacgg
     2521 caggtacaca ccggcgtcgg tgaatgttgc gacgaccgag tgcggcccgg acgtggcgat
     2581 gatgatgtcg gagctgaacg ccccacccgc gcccacaacc agcggggtcg ggttgagcgg
     2641 ggtgctcgaa ccattgtcga acaggttcac ggtgccagcg gtaaccggcg gcggcgtggt
     2701 gccgttaccg gtgacagtgc cgctgaagtg ggtcggggtc gatccacccg cgaacggctg
     2761 ccaagtcgcg gccagcgtcg tggtcgcagt cggcagcgga accgagatgt tgtagacgag
     2821 cgcggtcgat gccgaggcca acaacccagt gccgttgaac ggggtgaacg tcgccgtgaa
     2881 cgagtggttg ctcgggttgg tttcgttgat cgggcccgtc gttaccgtga acggggcact
     2941 ggagccagga gcgacgatct tggtgtcgat gaccgtggca ccgtccttga ccacgacagt
     3001 accgatgccg ccgatgtacc cactcgcgac cgagccggca ggcgtgacgg ttgtcgtcaa
     3061 cgtgacgggc agcgggttcg acgatgagat cgggctcgcc gggcttgccg agatcgtcgt
     3121 cgtcgtcgca accgtctggt cagggttcgg gacgagggtc caggtggtgc cgctgatcaa
     3181 gatgtcagcc gagtcgtacg tcgctgttgt tgaaccggtg aacaaccgca actggtacag
     3241 gttctgccag gtcggctgcg ccgtgttcgg cccagtcgtc gaggtgttcg ggaagtcagc
     3301 agcaaaggat gcgggcgaga ggtcggttga ccgctcgttg atcacaggct tgcccgcagt
     3361 caccaaaccc ttgaggtcgg ttgggtaggc agcaggcgcc gggctgaaca cggtgtcaag
     3421 tccgactgct tcgcctgacc acagtgcggt gggctggcca aacgccggca ggtagacgtt
     3481 tagggacgcg tcagtgtcgc ccgcgttgat gagcttcgaa ccagccgcgt aggcggcgat
     3541 cggcacggtg gtgctgccgc tcgtcaccgg cacgccgttc gcgtcggtaa aggtgatcga
     3601 accgagcgag cccgagtcgg ggttgtaggg cgccgcagcg aaggcggtgc ccgcagtagc
     3661 gagcgtcacc ccagccgcga tcgcgacggc gccgactgcg gctaccgcgc gctttggatg
     3721 caacttcatt tcgtcctctt tctgcgaagt catgaacagg tgtgagtcag gtgtgagtac
     3781 ggctcaaagc aactgcttca aatccgtgcg gccttggagc ggcggccttg ctccagcacc
     3841 ggctcccgga ggaggccggt gccggagcga agaccacgtc gatcagctga tggagctggg
     3901 attgcggcta gctgaacgcg gggcaggtcg ctgcgccgtc ggctgcgccg cagtcgacgt
     3961 agccgccaac gaaaccggct gcggtcacgt ccgcctggcc ggttgcccga gagaggtaac
     4021 tgctggagcc gacgaacagc gtcttggccc agttgttagc gccacccggc tcccacacgg
     4081 tcccgttgtt cacgtcgatg tcgcgaaccg tgatgtacat gttgcgggtg agctggtaga
     4141 tcgggttcgt gtcggtcggg gtaccggacg acgcgaccac cttgttggcc gctacgcccg
     4201 cgttggcgaa gtagccggag ttgatcagcg caatgcgggc tgccgagaac ggctcgattg
     4261 cgtcgatcgc gttcgggttc agaacgatgc cgtcagggtc gtgctcctgg gcagtctgta
     4321 cacaactacc gagtgggccg gtcgaggtcg gaagtccgta gtgagcgttg aggtcagcca
     4381 agaagtcgtt gcgcgtacct gacccgctct gcggaatgat cgggacgatc gagtcggacg
     4441 aggtgccgcc gatgcccggg tcgttccagt gagtgaagcc gggaaggcac tggtagattt
     4501 tggcgagctg gacacccgac agcgccaccg cgttggagcc gcccagcgac gggtc
//
LOCUS       DASWVF010000253         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_134406, whole genome shotgun sequence.
ACCESSION   DASWVF010000253 DASWVF010000000
VERSION     DASWVF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134406"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VGD55_04520"
     CDS             complement(<1..490)
                     /locus_tag="VGD55_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869638.1"
                     /translation="MMLPAANSGSLRSFVGRWGWRPWHYALLLLIHAHFFTSDGLGTG
                     RDMVDVFYPWADAVRLSILKFHQFPWWNPWAMAGQPLFADPASVAVLMPDTLLVLIFG
                     AVTGLKIAIVLYVLVGYEGSRVLCRHLFGTSRFVEGASVIPAIIPTLALHFNEGHIIF
                     VMF"
     gene            complement(487..1359)
                     /locus_tag="VGD55_04525"
     CDS             complement(487..1359)
                     /locus_tag="VGD55_04525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869639.1"
                     /translation="MTCVICGASAIEEVRPADIERLEEVSFSYSFRPEHTRTFRIVRC
                     KACTHMFCAPLPARVTESYHDVVDEEYLKHAESRRLAAELVLRTVVQSTVGRSLLDVG
                     CATGDFLEAGRQAGFEAEGVELSSWSCAIARQRGFVVHDKPLADFAKEGGQRFDVVSL
                     IGVIEHFPDPRAEMENIAKLLRPGGIVVIWTGDASAWLPRLLGRRWWYWQGQHIQYFT
                     QASMERLAALTGFEQVTTERYPFAATASTISNSLRRYPLHRVLSAFTNLVFKLKPVIT
                     LRLPGEMLFIARRK"
     gene            1449..>2166
                     /locus_tag="VGD55_04530"
     CDS             1449..>2166
                     /locus_tag="VGD55_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0869640.1"
                     /translation="MGMHLVHRKTCRVCHSAALTKVIDLGDQYLQGSFVKEGRQSPPM
                     RLVSTSVVRCDPMADEKACGLLQMEYTVPPEVLYSVYWYRSGTNATMRNHLAGIANEA
                     TALVGKTSGRVLDIGCNDGTLLAAFPKGFTKFGIDPSDLAREVPADIKVIHELFPSEE
                     LRSILKGELCDVVTSIAMFYDLEDPIAFAKAIKEILAPDGIWCFEMSYMPTMLEMTSY
                     DTICNEHLEYYSLAVLEYILK"
BASE COUNT          416 a          656 c          729 g          365 t
ORIGIN      
        1 agaacatcac gaagatgatg tggccctcgt tgaaatgaag tgcgagggtc gggatgatcg
       61 ctggaatgac gcttgcgccc tcgacgaagc ggctcgtccc aaagaggtgg cggcagagca
      121 cgcgcgagcc ctcgtagccg acgaggacgt agagcacgat cgcgatcttg agcccggtga
      181 cagcaccgaa gatcaggacg agcagcgtgt ccggcatgag cacggccacc gacgctggat
      241 cggcgaagag cggctgcccg gccatcgccc agggattcca ccaggggaac tggtggaact
      301 tgaggatcga caggcggacg gcgtcggccc atgggtagaa cacgtcgacc atgtcacggc
      361 ccgtgccgag accgtccgag gtgaagaaat gcgcgtggat cagcagcagg agcgcgtagt
      421 gccacggacg ccacccccac cgtccgacga acgagcgcag ggagccgctg ttggccgccg
      481 gcagcatcac tttcgccggg cgatgaacag catctcgccc ggaagtcgga gcgtgatgac
      541 gggcttcaac ttgaaaacga ggttggtgaa cgcggagagg acgcggtgca gagggtagcg
      601 tcgcagcgag ttggagatcg tcgacgccgt ggcggcgaac gggtaacgct cggtggtgac
      661 ctgttcgaac ccggtgagcg ccgcgagccg ctccatgctg gcctgcgtga agtactggat
      721 gtgctgcccc tgccagtacc accagcgacg cccgagaaga cgcggcagcc aggcgctcgc
      781 gtccccggtc cagatcacga cgatgccgcc ggggcgcagg agcttggcga tgttctccat
      841 ctcggcgcgg gggtcgggaa agtgctcgat gacgccgatc agtgacacga cgtcgaagcg
      901 ctggcccccc tccttcgcga agtcggccaa cggcttgtca tgcacgacga aaccgcgctg
      961 ccgggcgatc gcgcacgacc agctcgacag ctcgaccccc tcggcctcga agccggcctg
     1021 gcgtcctgcc tcgaggaaat cgcccgtggc gcagcccacg tcgaggaggc ttcgtccgac
     1081 ggtgctctgg acgacggtgc gcaacaccag ctcggccgcg agccgccgcg actcggcgtg
     1141 tttgaggtat tcctcgtcga cgacgtcgtg gtagctctcc gtcaccctgg ccggcagggg
     1201 cgcgcagaac atgtgcgtgc aggccttgca acgcacgatg cggaacgtcc gcgtgtgttc
     1261 cgggcgaaag gagtacgaaa acgaaacctc ttccagacgt tcgatgtcgg cgggccggac
     1321 ctcttcgatc gcggacgcac cacagatgac gcaggtcatg ggcgcaccct tttagatcaa
     1381 gtggacggaa attgcccgcc ttcgctgccc ggcggctgct tgaaattggg ccggcagaag
     1441 tgttatcgat gggcatgcat ctcgtccacc ggaagacctg ccgggtttgt cactcggcgg
     1501 ctttgaccaa ggtcatcgat ttgggtgacc agtacctgca ggggtcgttc gtgaaggagg
     1561 gccggcagtc gccgccgatg cgcctcgttt cgacctcggt ggtgcgctgc gatcccatgg
     1621 cggacgagaa agcctgcgga ctgctgcaga tggagtacac ggtcccgccc gaggtcctgt
     1681 actcggtgta ctggtaccgg tcggggacga acgcgaccat gcgcaaccac ctggccggca
     1741 tcgcgaacga ggccacggcg ttggtgggca agacaagcgg tcgggtgctg gacatcggtt
     1801 gcaacgacgg gacgttgctg gcggccttcc cgaagggttt cacgaagttc ggcatcgatc
     1861 cgtcggacct ggcgcgcgag gttcccgccg acatcaaagt gattcacgag ctgttccctt
     1921 ccgaggagct gcgttcaatc ctgaagggtg agctttgcga cgtcgtgacc tcgatcgcga
     1981 tgttctacga cttggaggat ccgatcgcct ttgccaaggc gatcaaggag atcctggccc
     2041 ctgatgggat ctggtgcttc gagatgtcgt acatgccgac gatgctcgag atgaccagct
     2101 acgacaccat ctgcaacgaa cacctcgagt actacagctt ggctgtgctc gaatacattc
     2161 tgaaac
//
LOCUS       DASWVF010000254         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_338069, whole genome shotgun sequence.
ACCESSION   DASWVF010000254 DASWVF010000000
VERSION     DASWVF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338069"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VGD55_04535"
     CDS             <1..698
                     /locus_tag="VGD55_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY0869641.1"
                     /translation="PYWDFNAAGIPNEPHDTSAAAITASALLQLAFLEPDPTRAATYL
                     AAARSTLQSLMTPQYSGAATQGILTDAVYNRQQATQFGLATVWDDYYFEEALLRLRWF
                     APTGGAAAVSAVSASVDQGDATNVIDSNASTAWTSSDDGDWVELALSNSTVLHAVTVQ
                     LVNGTNVSASLEFQLSTDGSSWTTVARALSSGQVATPETYTFAPVTANYVRVVVHGSS
                     AGPAVAVGGVVAY"
     gene            736..1530
                     /locus_tag="VGD55_04540"
     CDS             736..1530
                     /locus_tag="VGD55_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0869642.1"
                     /translation="MTTDSRVALVTGASQGIGRVVSTHLRAAGYEVAAIARSQPLLAE
                     LAAETGAMPVVLDVTDAQAVAGAVAHVEAELGPISLLVNNAGISGNGGASWEHEPADW
                     WRVFEVNVLGSFLLCHAVVPRMVERRSGRVVNLSSNAAFFAIDDDFEPILSSAYLASK
                     AAVIRFSEALAGEARPAGVGVFAISPGMVKTDMTAGPFADDWDDPELWSPPELAADLV
                     EFIGSGAFDELAGRYIHAAADDWRHLMERTPEIIENDLYAMRVRKL"
     gene            1646..2059
                     /locus_tag="VGD55_04545"
     CDS             1646..2059
                     /locus_tag="VGD55_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869643.1"
                     /translation="MTRRTGLVVSGAVVVIGLLAAAVVVGRATAPVRDRGFANGDAAG
                     YAEGVAVGRALQIGDSVPKGSAAATTAAFQAGYRAGETDAYGSYDGGWKLGAPYVVVL
                     ASGLGGAPYRIIDRDQLILGTTYRLCPDGTSICHS"
     gene            complement(2158..3627)
                     /locus_tag="VGD55_04550"
     CDS             complement(2158..3627)
                     /locus_tag="VGD55_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869644.1"
                     /translation="MTTSMQAPVAIPGAARRGHHPGLALAVIATSQLMVVLDATIVNV
                     ALPTIQTHLKFSYTSLTWVLNAYTLTFGGLLLLGGRAGDILGRRRVFIFGIALFTFAS
                     FLGGLATASWWLLAARALQGVGGAIASPTALSLITTNFEEGAERNRAFGVFAAVSGSG
                     AAIGLLAGGMLTSWASWRWVLFVNVPIGIFLVSITRQYISESEKHPGRFDLVGAITST
                     LGMTSLVYAFIRASGSAGWGDSVTLLCFAAAVILLVAFLVNEMRSPQPITPLHMFKNR
                     NRSGSYVVMLFLAGALFAMFFFLTVFVQRVLHYSPLKTGFIYLPLSVAIVISAQICAK
                     QLIKVGPKPFMVAGAVMSAVALWWLSLVNVHSGYVANLLGPLVIFGLGMGFLFVPLTM
                     IAVSGVEPEEAGSASGLLNVMQQVGGSLGLAILTTAFATAQRHAAAHGATGNAKLAHG
                     ISTALQVSVVFGICALLASIFLITAKASDLDPSAMPGMG"
     gene            3755..4462
                     /locus_tag="VGD55_04555"
     CDS             3755..4462
                     /locus_tag="VGD55_04555"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0869645.1"
                     /translation="MPAARSRSLTTLATGTDKKAGAPQDASTIKDPPSLRRLCRRRGA
                     ALETAIYEAVLQQLSTVGFAAMTIEGIAATARTGKAAIYRRWPSKEELVADTLDNVLP
                     SLDLPPDTGNVRTDIARIFDLMTATMESPAGGAMQALLGELGHDHEFIKTLHVRVLAP
                     RKALMLEILRRGVERGDVKPDAVKPVIAEAGPALLVHRLLMYGPPIDPAYVDAILDDV
                     VMPLISPAWKEPSRARS"
     gene            complement(4443..>5061)
                     /locus_tag="VGD55_04560"
     CDS             complement(4443..>5061)
                     /locus_tag="VGD55_04560"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0869646.1"
                     /translation="QSAGAGSFGGDFLVAALTGGGGVLEVVLVDVSGKGIDAGTRALL
                     LSGALGGLLGALPPDHFLPAANDYLLRQDWGEGFATAVHLVVDLSTGSYVVESAGHPP
                     AAHFVKGSGTWRVIDSEGTVLGVLPQASYTSSTGVLGPGDAMLLYTDGVVETSEREIA
                     VGIDKLIGEAERLVTQGFRDGAKHLVDRVAPHGSDDRALVMIWRD"
BASE COUNT          981 a         1641 c         1610 g          829 t
ORIGIN      
        1 tcccgtattg ggatttcaat gccgccggca taccgaacga gccgcacgac acctcggccg
       61 ctgcgatcac cgcgtcggct ttgctccagc tcgcgttcct ggagcccgac ccgacgcgcg
      121 ctgcgacgta cttggcagca gctcgttcga cgttgcagtc gttgatgaca ccgcagtatt
      181 cgggtgctgc gacacaaggg attctcaccg atgccgtcta caaccggcag caggctacgc
      241 agttcgggct cgctaccgtg tgggacgact actacttcga ggaagccctg ctgcggctgc
      301 gatggtttgc accgacgggt ggtgcggctg cggtttcggc ggtgtcggcg agcgttgatc
      361 agggtgacgc gacgaacgtg attgacagca acgcatcgac agcgtggacg tcgagtgatg
      421 acggtgactg ggtcgagctt gcgctgtcga acagcacggt tttgcatgcg gtaaccgtgc
      481 agttggtgaa tggcacgaac gtgtcggcgt cgctggagtt tcagctgtcg accgacggct
      541 cgagctggac gacggtcgcg cgagcgttga gcagcggaca agttgctacg ccggagacct
      601 acaccttcgc gccggtcacc gcgaactacg ttcgcgtcgt cgttcacggc agctcagccg
      661 gtccggcggt tgcggtcggc ggcgtcgtcg cgtactgacg ccctcctccc gcttgccgct
      721 gataccgtct gccccgtgac gaccgattct cgggtagcac tcgtgaccgg tgcgagccag
      781 ggcatcggcc gtgtcgtcag cacccacctc cgtgccgcgg gttacgaagt cgccgccatc
      841 gcgcgctcgc agccgctcct cgcagagctt gccgccgaga ctggcgcgat gcctgttgtg
      901 ctcgacgtca ccgatgcgca ggccgtggcc ggggcagttg cacacgtgga agccgaactc
      961 ggcccgatct cgttgctcgt caacaatgca gggatttccg gcaacggcgg cgcgtcttgg
     1021 gaacacgagc ctgcggactg gtggcgcgtg ttcgaggtca acgttctcgg gtcgttcctg
     1081 ctatgccacg cagtcgtgcc acggatggtc gagcgccggt ccggaagggt cgtgaacctc
     1141 tcgtcgaacg cagcattctt cgcgatcgac gacgacttcg aaccgatcct cagttcggcg
     1201 tacctggcaa gtaaggcggc tgtcatccgg ttcagtgaag cgcttgccgg agaggcgcga
     1261 ccggccggcg tcggcgtgtt cgcgatctcg ccaggaatgg tgaagaccga catgaccgcg
     1321 ggtccattcg ccgacgactg ggatgatccc gagctgtggt ccccacctga gttggcagct
     1381 gacctcgtcg agttcatcgg gtccggcgct ttcgacgagc ttgcgggtcg ctatatccat
     1441 gcggctgccg acgactggcg ccaccttatg gaacgcaccc cggagatcat cgaaaacgac
     1501 ttgtacgcca tgcgggtccg caagctgtaa cgcgtctgga tacgcaagcc ttggcgtccg
     1561 gcgggtcgtc agttggagcg gctcggccaa ccgcgacgcc ctcccgtccc tcgtcagcgt
     1621 gcgcgctcta cggcaaggtg ggcgcatgac gagacgtacg ggattggtgg tgtcaggcgc
     1681 cgttgtcgtg atcgggttgc tcgctgccgc ggtcgttgtc ggccgagcga ccgcgccggt
     1741 tcgcgaccgc gggtttgcga acggcgacgc ggctggctac gccgaggggg tggcggtggg
     1801 tcgcgcgctg cagatcggcg acagcgtgcc taaggggtcg gcggcggcaa cgaccgcagc
     1861 gtttcaggct ggctaccgcg ctggtgagac cgacgcgtac ggctcatatg acggcggctg
     1921 gaaactcggc gcgccttacg tagtcgtgct cgcgtcggga cttggcggag caccgtaccg
     1981 catcatcgat cgtgaccaac tgatcctggg aacgacgtac cggctctgcc cagacggaac
     2041 ctcgatctgc cacagctagc tcggcaagca cctggctgca acaacgtgga gtgtgcgggt
     2101 cgctagaaca acctccacac tccacgttcc caaggcgccg cccgccacgg cgccgagcta
     2161 acccatcccg ggcatcgcgg acggatcgag gtcagatgcc ttcgccgtga tcaagaagat
     2221 cgacgcaagc agcgcgcaga taccgaacac gaccgagacc tgcaatgcgg tcgagatccc
     2281 gtgcgccagc ttcgcgttgc ccgtcgcgcc gtgggcagcg gcatgccgct gcgcggtggc
     2341 gaaggcagtg gtgagaatcg ccagcccgag cgacccgcct acctgctgca tgacgttcag
     2401 cagacctgac gccgagcctg cctcttccgg ctcgacgcct gagaccgcga tcatcgtcaa
     2461 cggcacgaac aggaagccca tgccgagacc gaagatgacg agtgggccga gcaggttcgc
     2521 gacatacccg ctgtgcacgt tcaccagcga cagccaccag agcgcaactg cggacatcac
     2581 ggcgccggcc accatgaacg gcttcgggcc gaccttgatg agttgcttcg cgcagatctg
     2641 tgcgctgatc acaatcgcca cgctgagcgg caggtagatg aagccggtct tcaacgggct
     2701 gtaatgaaga acacgctgaa cgaagaccgt aaggaagaag aacatcgcga acagcgcgcc
     2761 ggctagaaag agcatgacga cgtaactacc ggatcgattg cgattcttga acatgtgcag
     2821 tggcgtgatc ggctgcgggc tgcgcatctc attgacgagg aacgccacga ggaggatcac
     2881 ggccgccgca aagcagagca gcgtcaccga atcgccccag ccggcagacc cggacgctcg
     2941 aatgaacgca tagacaagtg aggtcatgcc gagcgtcgag gtgatggcgc ccacgaggtc
     3001 gaagcggcca gggtgcttct ctgactcgct gatgtactgg cgggtaatgc tgacgaggaa
     3061 gatgccgatc ggaacgttca cgaacagcac ccagcgccaa gaagcccacg acgtcaacat
     3121 gccgccagca agcagaccga tggcagcacc tgagcccgac acggcagcga atacaccgaa
     3181 tgccctgtta cgttctgcgc cctcttcgaa gttcgtggta atcagcgaca gcgccgtcgg
     3241 ggaagcgatg gcaccgccca cgccttgcag tgcgcgcgcg gcgagcaacc accacgacgc
     3301 ggtggccagc ccaccgagga acgaggcgaa cgtgaacagc gcgatgccga agatgaacac
     3361 gcgccgccga cccagaatgt cgccagcgcg gccaccgagc aggagcaaac cgccaaacgt
     3421 gagtgtgtag gcgttgagca cccaggtaag gctcgtgtac gagaacttca ggtgagtctg
     3481 aatggtcggg agcgcgacgt tgacgatcgt tgcgtcgagc acgaccatca actggctggt
     3541 ggcaatgacc gcgagagcaa ggccggggtg atggccacgc cgcgctgcgc ccggaatggc
     3601 gactggtgcc tgcatcgagg tagtcatgat tctccacact tggctcgaat gaacgctacc
     3661 gttcactaat gacggacgct acattgcacg ggatagagaa cgcaaccgtt cattccgaac
     3721 cgagctaaac cgatctgcac ccgaggaggt cgccgtgccc gccgcacgtt cgcggtcgtt
     3781 gacgacgcta gcaacgggca ccgacaagaa agctggcgcg ccccaggacg ccagcacgat
     3841 caaggacccc ccgtcgctgc gacggttgtg ccgtcggcgc ggcgccgcgt tagagactgc
     3901 catctacgaa gccgtcctcc agcaattgag caccgtcggg ttcgctgcga tgacgatcga
     3961 gggcatcgct gccaccgctc gcaccggcaa ggccgctatc tatcgccggt ggccctcaaa
     4021 ggaagaactg gtcgccgaca ccctcgacaa cgtgctcccc tcgctcgatc ttccccccga
     4081 caccgggaac gttcgcaccg acatcgccag gatctttgac ctcatgacag cgacgatgga
     4141 gagcccggcc ggtggcgcca tgcaggcact gctcggcgaa ctcggtcacg atcacgagtt
     4201 catcaagacg ttgcacgtgc gggtgttggc gccgcgcaag gcgctgatgc tcgagatcct
     4261 gcgtcgcggc gtcgagcgcg gcgatgtcaa gccggacgcc gtcaaacccg tgatcgctga
     4321 ggcaggtccg gcactgctcg tgcaccgatt gctgatgtac ggaccaccga tcgaccccgc
     4381 ctacgtcgat gcgatcctcg acgacgtcgt gatgcccctc atcagccccg catggaaaga
     4441 acctagtcgc gccagatcat gacgagcgcg cggtcatcgc ttccgtgcgg cgcgacccga
     4501 tcgaccaggt gcttcgcacc atcacgaaat ccctgtgtca caagacgttc tgcctcaccg
     4561 atcaacttgt cgatgccgac ggcaatctca cgttctgacg tctcgacgac gccgtccgtg
     4621 taaagcaaca tcgcgtcgcc cggaccaagc acaccggtcg acgacgtgta gctcgcttgc
     4681 ggaagaacgc cgagcacggt cccctcgctg tcgatcaccc gccacgtacc actgccctta
     4741 acgaagtgcg ccgcaggcgg atgacccgcg gactcaacca cataactgcc agtactcaaa
     4801 tcgacaacaa ggtggacggc ggttgcgaac ccttcgcccc aatcctgacg aagcagatag
     4861 tcgttggctg caggcaagaa gtggtcgggc gggagcgcgc cgagcagacc tccgagagca
     4921 cctgacaaca acagcgcccg cgtgccagcg tcgatgccct tacccgagac gtcgacaagc
     4981 acaacctcga gcaccccgcc gccaccggtc agcgccgcga ccaggaaatc tccgccgaac
     5041 gagccggcgc ccgctgactg g
//
LOCUS       DASWVF010000255         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_134470, whole genome shotgun sequence.
ACCESSION   DASWVF010000255 DASWVF010000000
VERSION     DASWVF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134470"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..463
                     /gene="rpsH"
                     /locus_tag="VGD55_04565"
     CDS             56..463
                     /gene="rpsH"
                     /locus_tag="VGD55_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0869647.1"
                     /translation="MMMTDPIADMLTRVRNANHAYHDNVPIPYSKIKTHVADILKEQG
                     YIAGWHVEDVEGHVAAKNLVVELKYGPNRERSIAGVRRVSKPGLRVYAKGTNLPRVLG
                     GLGIAIISTSGGLLTDKQCAKRGVGGEVLAYVW"
     gene            481..1020
                     /gene="rplF"
                     /locus_tag="VGD55_04570"
     CDS             481..1020
                     /gene="rplF"
                     /locus_tag="VGD55_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0869648.1"
                     /translation="MSRIGRLPIPVPSGVDVDINDRLVTVKGPKGTLTHTLADPIVIE
                     KADDGSLLVTRPDDEKRSKERHGLSRTLVANMVTGVTDGFSKTLEIVGTGYRVQAKGS
                     DLEFALGFSHPVNIPAPDGITFEVQAPTRFVVRGIDKQQVGEVAANIRKLRKPEPYKG
                     KGVRYQGEVVRRKVGKAGK"
     gene            1023..1412
                     /gene="rplR"
                     /locus_tag="VGD55_04575"
     CDS             1023..1412
                     /gene="rplR"
                     /locus_tag="VGD55_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0869649.1"
                     /translation="MPVGLISRRKARGSTKSALRQRRHFRVRKRVTGTAERPRLVVNR
                     SSRHVFAQVVDDVTGHTLASASTMEADVRATAGDKTAKARKVGELVAKRAAAAGVGPV
                     VFDRGGYQYHGRVAAIADGAREGGLTL"
     gene            1498..>1788
                     /locus_tag="VGD55_04580"
     CDS             1498..>1788
                     /locus_tag="VGD55_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0869650.1"
                     /translation="MPGPQRRGPGSGAGTGAGAAGGPGGTGSGSGGGDRRDRRDNRRP
                     DGAAEKTQYIERVVAINRVAKVVKGGRRFSFTALVVVGDGDGSVGIGYGKAKE"
BASE COUNT          346 a          569 c          595 g          278 t
ORIGIN      
        1 tcagggtgag aaccacgacg aggcgaaacc acggcgagaa aggccatgtg gccacatcat
       61 gatgactgac ccgatcgcgg acatgttgac gcgagtgcgc aacgcgaacc acgcgtacca
      121 cgacaacgtg ccgattcctt attcgaagat caagacgcac gtcgccgaca tcctcaagga
      181 gcagggctac atcgccggct ggcacgtcga ggatgtcgaa ggccacgttg ccgcgaagaa
      241 cctggtcgtc gagctcaagt acggcccgaa ccgtgagcgc tcgatcgctg gcgtccggcg
      301 ggtcagcaag cccgggctgc gcgtttacgc caagggcacc aacctgccgc gcgttctcgg
      361 cggcctcggc atcgcgatca tttcaacatc cggcgggctg ctgaccgaca agcagtgcgc
      421 aaagcgtggg gtaggcgggg aagtcctcgc ctacgtctgg taacaggaag agacgaactc
      481 atgtcccgaa tcggaagact tccgatccct gttcccagcg gggtcgacgt cgatatcaac
      541 gaccgcctgg tcacggtcaa gggcccgaag ggcaccttga cgcatacgct cgccgatcca
      601 atcgtcatcg agaaggcgga cgacggatcg ctgctcgtta ctcgccccga cgacgagaag
      661 cgcagcaagg aacgccacgg gctcagtcgc accttggtcg cgaacatggt gaccggagtg
      721 accgacggct tctcgaagac gttggagatc gtcggtaccg gttaccgcgt gcaggcgaag
      781 ggctcggacc tcgagttcgc gctcggcttc tcgcacccgg tcaacatccc cgcgcccgat
      841 gggatcacct tcgaggtgca ggcgccgacg cgcttcgtcg tgcgtggcat cgacaagcag
      901 caggtcggtg aagtcgcagc caacatccgc aagctgcgca agcccgagcc ttacaagggc
      961 aagggcgttc gttaccaggg tgaagtcgtg cgtcgcaagg tcggaaaggc tggtaagtag
     1021 tcatgcctgt tggattgatc agtcgccgca aggcgcgcgg atctaccaag tcggccctgc
     1081 gtcagcgccg ccactttcgg gtccgcaagc gcgtcaccgg caccgcggaa cgcccgcgcc
     1141 tcgtcgtcaa ccgttcgagt cgtcacgtgt tcgcgcaggt cgtcgacgac gtgaccggcc
     1201 acaccctcgc ttcggcttcg accatggaag cagacgttcg tgcgaccgcg ggcgacaaga
     1261 cggccaaggc tcgcaaggtc ggtgaactcg tggcgaagcg tgctgccgct gcaggcgtcg
     1321 gcccggtggt cttcgaccgt ggtggatacc agtaccacgg tcgggtcgcg gcaatcgccg
     1381 acggcgcccg cgagggcggc ctgacgctgt gacgatcacc caccgctcga ccttcaagcg
     1441 ctcgaccttg aaccgcgtgg ccctgaactg cgtgaacact gagatgagga acgtctgatg
     1501 ccaggacctc agcgtcgtgg acccggaagc ggcgccggca ctggtgccgg cgcggctggc
     1561 ggacccggcg gaaccggcag tggcagcggt ggcggggacc gccgtgaccg ccgtgacaat
     1621 cgtcgtcccg atggcgcggc cgagaagacc cagtacatcg agcgcgtcgt cgcgatcaac
     1681 cgtgtcgcca aagtcgtgaa gggcgggcgg cgcttcagtt tcactgcgct cgtagtcgtc
     1741 ggtgacggcg acggcagcgt cggcatcggt tacggcaagg ccaaggaa
//
LOCUS       DASWVF010000256         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_363523, whole genome shotgun sequence.
ACCESSION   DASWVF010000256 DASWVF010000000
VERSION     DASWVF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363523"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VGD55_04585"
     CDS             complement(<1..474)
                     /locus_tag="VGD55_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0869651.1"
                     /translation="MFIARRNRRALVGVVAVAALAVAGCSSKAKTASAPSSSAPATTT
                     SAPVAASTSAAAPSVAASSAPASSAPAETITIGLFGTFGFKEAGLYDGYMKLHPNITI
                     KEDDVEKSDDYYKALQTHLAAGSGLDDIQGIEIGFVADVTANHADQFVDFNSLPEG"
     gene            957..>1787
                     /locus_tag="VGD55_04590"
     CDS             957..>1787
                     /locus_tag="VGD55_04590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0869652.1"
                     /translation="MSAVLSDFARTLATDFPIQGILDHLVRRIVDVLPVTGAGVTVIS
                     AESGPHYIAASDDSALRFEQLQSDLGDGPCVWAYRSGEPVAVPDLAGDDRFAQFGPPA
                     VATGLAAVFTFPLRHGKGRLGALDLYRDTVGALDRGDMAAAQTLADVAAAYLLNAQAR
                     EEARVTSERFRHTALHDPLTGLPNRLLLQQRLEHAAQRAQRSHTNAAVLFVDLDRFKR
                     VNDTHGHRVGDDLLCAIAERLTGLVRPGDTLARFSGDEFVFLCEDLTSAADVEALAER
                     V"
BASE COUNT          304 a          563 c          579 g          341 t
ORIGIN      
        1 accttcaggc aacgagttga agtcgacgaa ctggtcggcg tgattcgccg taacgtctgc
       61 gacgaagccg atctcgatgc cctggatgtc gtcgagccca gacccggctg caaggtgggt
      121 ctgcaacgcc ttgtagtagt cgtccgactt ctcgacgtcg tcttccttga tggtgatgtt
      181 ggggtgcagc ttcatgtacc cgtcgtagag cccggcctcc ttgaagccga acgtgccgaa
      241 cagcccaatg gtgatcgttt cagccggggc gctcgatgcg ggcgccgacg atgctgcgac
      301 ggagggcgcc gccgctgatg ttgaagctgc gaccggcgcg gacgtcgtgg tggctggcgc
      361 actactcgat ggcgctgaag cggtctttgc cttcgatgag cagccggcga ccgcaagggc
      421 cgcgaccgcg acgacgccga caagcgcacg ccgatttctc cgagcaatga acatggatcc
      481 tcctggggtc ctccgcgagg acccgctcgt tgaagccacc cgatttgggg accttcgaag
      541 ctgatcttgc gtgggcacgt tcccacgcac gatgaagcat gagtgtcgaa gtgacgcgac
      601 cggctgtcaa ggtcgcggag tcggctcgcc acgatcgagg cggtttcgtt atagaaaccc
      661 gatctatatg ggcgataaac gctgctttgg gcaatggaac ggtcgcgcca atcgccgcct
      721 tcgcgttgct ggcggaggct cgccgaggca accgcttcga tggccggcca actggtgatc
      781 gatgggaacg atccccagta gttgaagctc gagctatcac gaaccgggcg gatcggacat
      841 tccggcacag ggggctgttt catgctggta ctgtcggaac gttgatcttc ccgtccggac
      901 cacctgcggg cctgccttag cggcgccgag gggtgtctgt ggtcgacgag ggcaaaatgt
      961 ccgccgtgct gagcgatttc gcgcgcaccc tagccaccga ttttccgatc caggggatct
     1021 tggaccacct ggtgagacgg atcgtcgatg tgctgccggt gacaggggcc ggtgtcacgg
     1081 tgatctcagc tgagagcggt ccccactaca tcgccgcctc cgatgattca gcgctgcgtt
     1141 tcgagcagct gcaaagcgat ctcggcgacg gcccgtgtgt gtgggcctac cgctccggtg
     1201 agccagtcgc cgttcccgac cttgcgggtg acgatcggtt cgcacagttc ggaccgcccg
     1261 cagtagcaac cgggctcgcc gccgttttca ccttcccgct tcggcacggg aaagggcggc
     1321 tcggggccct cgacctctat cgcgacactg tgggcgcact tgatcgcggt gacatggctg
     1381 cggcacaaac gctcgctgac gtggctgctg cgtacctctt gaacgcgcag gcgcgagaag
     1441 aagcgcgcgt gacgtccgaa cgatttcggc ataccgcctt gcacgatcca ctcaccgggc
     1501 tgccgaaccg gctcctgctc cagcagcgcc tcgagcatgc ggcgcaacgt gcgcaacgct
     1561 cgcacaccaa tgccgcagtg ttgttcgtcg acctcgaccg cttcaagcgc gtgaacgaca
     1621 cccacggaca ccgagtcggc gacgatctgc tgtgcgcgat tgccgagcgc ctgactgggt
     1681 tagtgcgacc cggtgacacc ctcgccaggt tctccggcga cgagttcgtc tttctctgtg
     1741 aggaccttac gagcgcggcc gacgtcgaag cgctcgcaga gcgcgtc
//
LOCUS       DASWVF010000257         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_440315, whole genome shotgun sequence.
ACCESSION   DASWVF010000257 DASWVF010000000
VERSION     DASWVF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440315"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VGD55_04595"
     CDS             complement(<1..370)
                     /locus_tag="VGD55_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869653.1"
                     /translation="MGTEAAVRRSALVAAEPAPAWPGPGTVTSVEQAPPGSCTIEPPF
                     SIPSTKLVLTSADQHWTVYLNIPGFPTNAINVGDTFDLAINARSVFWYGPEQLITLSK
                     SGKLVVFGYSGFQRPDLSAFG"
     gene            complement(557..>4556)
                     /gene="rpoC"
                     /locus_tag="VGD55_04600"
     CDS             complement(557..>4556)
                     /gene="rpoC"
                     /locus_tag="VGD55_04600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0869654.1"
                     /translation="KHRGIVCEKCGVEVIQSKVRRERLGHINLATPVAHIWFLKSLPS
                     RIGNLLDITLKDLEKILYCESYVVVDPKETGLVQGELLTEERYMKAAEEFGEDKFVAG
                     MGAEAIRDLLKEIDIHSLSEQLRAEMKEATSEAKRKKLSTRLKVVDAFRDSGNKPEWM
                     MLDVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLQELNAPEIIIRNE
                     KRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRS
                     VIVVGPELKLHQCGLPKKMALELFKPFIYNKLEKRGLVTTIKSAKKLVEKERPEVWDI
                     LEEVIKEHPILLNRAPTLHRLGIQAFEPLLIAGKAIQLHPLVCAAFNADFDGDQMAVH
                     VPLSLEAQMEARVLMMSTNNILSPANGRPIILPSQDIVLGLYYMTRERIFAKGEYPHG
                     RDEKKPKGDEKAEKISPTKMEGVYASPEEVRLAYDHGELDLQAAIRVRVDGEIIASTT
                     GRVLLYEIVPKQLPFKIVNRVMGKKQLGDLIDSCYRACGQKETVLLADKLRSLGYAHS
                     TKAGVSICVDDLLIPQRKIELLETAQKDVQEIEEQYTEGLITDGERYNKVVDIWAQVS
                     EQIAEEMMTEIGSEELFNDKTGEKRRSPSFNPIYIMADSGARGSAQQIRQLAGMRGLM
                     AKPSGEIIETPITTNFREGLSVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQ
                     DCIIAEYDCGTIDGIELGALVEGGEIIEKLGDRILGRVAVDDIFDPYTGEVLVASGEE
                     ITEDKVRIIEDAGIDHVKIRSVLTCETRRGICVLCYGRDLARGHMVNIGEAVGVIAAQ
                     SIGEPGTQLTMRTFHIGGVGQVRTEQSQLEARYDGIVKLENTDTTKREKEWIVMNRHG
                     EIVIQDETGRERERYGLTYGAKLHFADGDAVKAGQLLSEWDPFSVPILTEVTGVVKFG
                     DVIEGVTMTEQLDEVTGLSRKSITESKDAELRPRISLKDENGKTLKIPSGENDARYFL
                     PVGSTIAVNEGDHIQAGDVIARIPRETTKTKDITGGLPRVAELFESRKPKEHAVIAEI
                     DGVVSFGKDTKGKRKVLITPERGDAKEYLISKGKHLAVREGDKIRAGEPLMDGAANPH
                     DILKVLGEKALAKYLVDEVQEVYRLQGVKINDKHIETIVRQMLRRIRVMDVGDTTFLV
                     DEHVEKYLFEEENERVMTKGGKPAQGEPLLLGITKASLSTESFISASSFQETTKVLTE
                     AAVQGKVDYLRGLKENVIMGRLIPAGTGLGAYKRLSLHVDEAGADEQLPPAPLAASAG
                     ASAAAAPAAFDE"
BASE COUNT          689 a         1519 c         1451 g          897 t
ORIGIN      
        1 tcccaaacgc ggagaggtcg gggcgctgga agcccgagta gccgaacacg accagtttgc
       61 cgctcttcga gagcgtgatc agctgctcgg gcccgtacca gaagacgctc cgggcgttga
      121 tcgccaggtc gaaggtgtcg cccacattaa tagcgttggt cggaaaaccg gggatgttca
      181 ggtagacggt ccagtgctgg tccgctgacg tgagcacgag cttcgtggac gggatgctga
      241 acggcggctc gatcgtgcag ctacccggcg gcgcttgctc gacgctggtc accgtcccgg
      301 gtcccggcca cgccggcgcc ggctccgccg ccaccagagc cgacctgcgc accgccgcct
      361 ccgtgcccac cgggtcggtt cgggttgttc ggttggttgc tggaacaccc aaccgccatg
      421 acgtgccgct cgtgccccgc agatcctcgc ggtgcgcggc gcggacaggc gaaacgcgcg
      481 acatcaatgt catggcgcca aaaaaaacgg gaagccaggg tgacctgact tcccgttccg
      541 ttggacgcgt cgactgctac tcgtcgaagg cagccggtgc cgcggcggcg ctggcgcctg
      601 ccgaggcggc gagcggcgcg ggcggcaact gctcgtccgc gcccgcctcg tcgacgtgca
      661 gcgagaggcg cttgtaagca cccaggccgg tgccggccgg gatgagacgc cccatgatga
      721 cattctcctt gaggccccgc aggtagtcga ccttgccctg aaccgcggct tcggtgagca
      781 ccttcgtggt ctcctggaac gacgacgcgg agatgaagct ctcggtcgac agcgatgcct
      841 tcgtgatgcc gagcagcagc ggctcgccct gggcgggctt gccccccttg gtcatcacgc
      901 gctcgttctc ctcttcgaac aggtacttct cgacgtgctc gtcgaccagg aaggtcgtgt
      961 cgcccacgtc catgacgcgg atgcggcgca acatctggcg aacgatggtc tcgatgtgct
     1021 tgtcgttgat cttgacgccc tggagtcggt aaacctcctg gacctcgtcg accaggtact
     1081 tggcgagagc cttctcgccc aggaccttga ggatgtcgtg cgggttcgcc gcgccgtcca
     1141 tcagcggctc gccggctcgg atcttgtcgc cctcgcggac ggccaggtgc ttgcccttgg
     1201 agatgaggta ctccttggca tcgccccgct ccggcgtgat caacaccttg cgcttgccct
     1261 tggtgtcctt gccgaagctg accacgccgt cgatctcggc gatgaccgcg tgctccttcg
     1321 gcttgcgcga ctcgaacagc tccgcgacgc gcggcagacc gccggtgatg tccttggtct
     1381 tcgtcgtctc gcgcgggata cgcgcgatga cgtcgccggc ctggatgtga tccccttcgt
     1441 tgacggcgat ggtcgaaccg accggcagga agtaccgggc gtcgttctcg ccgctgggga
     1501 tcttcagggt cttgccgttc tcgtccttca acgagatgcg cggccgcagc tcggcgtctt
     1561 tcgactccgt gatgctcttg cgcgacagac ccgtcacctc gtccagctgc tcggtcatgg
     1621 tgacgccctc gatgacgtca ccgaacttca cgacaccggt gacctccgtg aggatcggca
     1681 ccgagaacgg atcccactcc gacaggagct gacccgcctt gaccgcatcg ccgtccgcga
     1741 agtgcagctt ggcgccgtag gtcaggccgt aacgctcccg ctcgcggccg gtctcgtcct
     1801 gaatgacgat ctcgccgtgg cggttcatca cgatccattc cttctcgcgc ttggtcgtgt
     1861 cggtgttctc cagcttgacg ataccgtcgt accgggcctc gagctgtgac tgctccgtgc
     1921 gcacctgacc gacgccgccg atgtggaacg tccgcatcgt cagctgcgtg ccgggctcgc
     1981 cgatcgactg ggcggcaatg acgccgaccg cctcgccgat gttgaccatg tgcccgcggg
     2041 ccaggtcgcg gccgtagcag agcacgcaga tgccccgccg cgtctcgcac gtcagcaccg
     2101 accggatctt gacgtggtcg atgcccgcgt cctcgatgat ccggaccttg tcctcggtga
     2161 tctcctcgcc ggaggccacc aggacctcgc cggtgtaggg gtcgaagatg tcgtcgacag
     2221 ccacgcgacc caggatgcgg tcgcccagct tctcgatgat ctctccgccc tcgaccagcg
     2281 cgcccagctc gatgccgtcg atggtgccgc agtcgtactc ggcgatgatg cagtcctgcg
     2341 ccacgtcgac cagacggcgg gtcaggtaac ccgagttcgc cgtcttgagc gccgtgtcgg
     2401 ccagaccctt gcgggcgccg tgcgtcgaga tgaagtactg gagcaccgac aacccctcgc
     2461 ggaagttggt ggtgatcggc gtctcgatga tctcgcccga cggcttggcc atcaggccgc
     2521 gcatgccggc cagctggcga atctgctgcg ccgaaccgcg cgcacccgag tccgccatga
     2581 tgtagatcgg gttgaacgac gggctgcgcc gcttctcgcc ggtcttgtcg ttgaacagct
     2641 cttccgagcc gatctccgtc atcatttctt cggcgatctg ctcggacacc tgcgcccaga
     2701 tgtcgacgac cttgttgtag cgctcgccgt cggtgatgag accttcggtg tactgctcct
     2761 cgatctcctg gacgtccttt tgcgccgtct cgagcagctc gatcttgcgc tgcggaatca
     2821 ggaggtcgtc gacgcagatc gagacacccg ccttggtcga gtgggcgtag cccagcgacc
     2881 gcagcttgtc ggccaagagg accgtctcct tctggccgca ggcccggtag cagctatcga
     2941 tcagatcacc gagctgcttc ttgcccatga cgcggttgac gatcttgaac ggcagctgct
     3001 tcgggacgat ctcgtacagc agcacgcgac cggtggtgct cgcgatgatc tcgccgtcga
     3061 cgcggacgcg gatcgccgcc tgcaggtcga gctcgccgtg gtcgtaagcc aggcgcacct
     3121 cttccggcga cgcgtacacg ccctccatct tggtcggcga gatcttctcg gccttctcgt
     3181 cgcccttggg cttcttctca tcgcgcccgt gcgggtactc gcccttggcg aagatccgct
     3241 cgcgcgtcat gtagtagagg ccgagcacga tgtcttgcga cggcaggatg atcgggcgcc
     3301 cgttggccgg cgacaggatg ttgttcgtgc tcatcatgag cacgcgtgcc tccatctgcg
     3361 cttccagcga cagcggcacg tgaacggcca tctggtcacc gtcgaagtcg gcgttgaacg
     3421 ccgcgcaaac cagcgggtgc agctggatgg ccttgcccgc gatcagcagc ggctcgaaag
     3481 cctggatgcc gaggcggtgc agtgtcggag cgcgattcaa gagaatcggg tgctccttga
     3541 tgacctcctc gaggatgtcc cacacctcgg gacgctcctt ttccaccagc ttcttcgcgc
     3601 tcttgatcgt ggtgacgaga ccacgctttt ccagcttgtt gtagatgaac ggcttgaaga
     3661 gctcgagcgc catcttcttg ggaagaccgc actggtgcag cttcagctcg gggccgacca
     3721 cgatgaccga acggccagag tagtcgacgc gcttgccgag caggttctgg cggaaccgcc
     3781 cctgcttgcc cttcagcatg tcgctgagcg acttcagcgg gcgcttgttc gggcccgtga
     3841 tggtcttgcc gcgacggccg ttgtcgaaca gagcgtcgac cgcctcctgc agcatccgct
     3901 tctcgttgcg gatgatgatc tcgggggcgt tcagctcttg cagcctcttc aaccggttgt
     3961 tgcggttgat gacgcggcgg tagaggtcgt tgaggtccga cgtcgcgaac cggccgccgt
     4021 ccaaggggac cagcgggcgc agatcgggcg ggatcaccgg gatcacgtcc agcatcatcc
     4081 actcgggctt gttgcccgag tcgcggaacg cgtcgacgac cttgaggcgc gtggacagct
     4141 tcttgcgctt ggcctcgctg gtggcttcct tcatctcggc gcgcagctgc tcgctgaggg
     4201 agtggatgtc gatctccttc agcagatcac ggatcgcttc ggcgcccatg ccggcgacga
     4261 acttgtcctc gccgaactcc tcggcggcct tcatgtagcg ctcttccgtg agcagctcgc
     4321 cctggaccag gcccgtctcc ttcgggtcga cgacgacgta cgactcgcag tagaggatct
     4381 tttccaggtc cttgagcgtg atgtccagca ggttgccgat gcgcgacggc agcgacttca
     4441 ggaaccagat gtgcgcgaca ggcgtcgcga ggttgatgtg ccccaggcgc tcacgacgca
     4501 ccttggactg aatcacctcg acgccgcact tctcgcagac gatcccgcgg tgcttc
//
LOCUS       DASWVF010000258         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_465735, whole genome shotgun sequence.
ACCESSION   DASWVF010000258 DASWVF010000000
VERSION     DASWVF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465735"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGD55_04605"
     CDS             <1..1187
                     /locus_tag="VGD55_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEY0869655.1"
                     /translation="PTVPGCETQCIKTNGGVETCDGFDNDCDGIVDDNLQPATITCLT
                     KGVCTGTQPTCGGQTGWVCNYPATYEPVEDTKFGCDGLDNDCNGQTDEPFQIGKTCAV
                     GSGACAGVGTWVCDNSMAGDHRCMGSMKTPSVEVCDGIDNDCDGLVDEIDSAWTSGPT
                     ADNPLRDVLVYVPSKNVTMYEFEASRYDATSTSAGFDSTKRGCSVPGKLPWANVTMSE
                     AEHACELLGPKWRLCTAPEWQAACEGTNPNPTIFPYGATYAPGSCNGADYQTGKGLTP
                     STIATGSAGACVSTISSTAKLFDMSGNVKEWVATDLTSTKPASNPSCTTPPCLFEMRG
                     GAYDISSFAVNGTTTAPGLQCDASVSAPYSTTVSGTTTTTQGIDVRLPSVGFRCCLPG
                     ML"
     gene            1190..>1664
                     /locus_tag="VGD55_04610"
     CDS             1190..>1664
                     /locus_tag="VGD55_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869656.1"
                     /translation="MTHHLRHRARALSGVALFGLLVGGATSARAQSLRPNIMFLFDTS
                     GSMHEDSNLVDRADGTTVCPQSTTSRIYSLKSGIRAALQQVGTDEANFGLMSFPQTVS
                     NTYTALTANQCTNTGQTPIGHYAATGTQTITVPNRVSTGFHNATSYSIGCLMTTNN"
BASE COUNT          314 a          582 c          527 g          241 t
ORIGIN      
        1 accccacggt tccgggttgt gagacccagt gcatcaagac caacggcggc gtcgagacct
       61 gtgacggctt cgacaacgac tgcgacggca tcgtggacga caacctgcag ccggccacca
      121 tcacctgcct gaccaagggc gtctgcacgg gtacgcagcc aacctgcggc ggccaaacgg
      181 gttgggtctg caactacccg gcgacctacg agcccgtcga ggacaccaag ttcggttgcg
      241 acggtctcga caacgattgc aacggccaga ccgacgaacc gttccagatc gggaagacct
      301 gcgcggtcgg aagcggcgcc tgtgcgggcg tcggcacgtg ggtttgcgac aactcgatgg
      361 ccggcgacca ccggtgcatg gggtcgatga agacgccgtc cgtggaagtc tgcgacggca
      421 tcgacaacga ctgcgacggt ctcgtcgacg agatcgactc agcgtggacg tccggcccga
      481 cggccgacaa ccctctgcgc gacgtgctgg tctacgtgcc gtcgaagaac gtgaccatgt
      541 acgagttcga ggcgagccgg tacgacgcga cgtcgaccag cgccgggttc gactcgacca
      601 agcgcggctg ctcggtcccc ggcaagctcc cgtgggccaa cgtgacgatg tccgaggccg
      661 agcacgcctg cgagctgctg gggccgaagt ggcggctctg cacggcgcct gaatggcagg
      721 ccgcctgcga ggggaccaac cccaacccca cgatcttccc gtatggcgcg acctacgcgc
      781 cgggtagctg caacggcgcc gactaccaga cgggcaaggg gctgacgccg tccaccatcg
      841 cgacgggcag cgccggcgcg tgcgtatcga cgatctccag cacggcgaag ctgttcgaca
      901 tgagcggcaa cgtgaaggag tgggttgcca ccgacctcac ctcgacgaag cccgcgagca
      961 acccgagctg cacgaccccg ccctgtctgt tcgaaatgcg aggcggcgcc tacgacatct
     1021 cgagcttcgc ggtgaacggc acgaccaccg caccggggct gcagtgcgat gcctcggtgt
     1081 cggcgcccta ttcgaccacc gtcagcggaa cgacgaccac cacccagggg atcgacgtcc
     1141 gcctgccctc cgtcgggttc cgctgctgcc tgccagggat gttgtagcca tgacccacca
     1201 ccttcgtcac cgcgcccgtg ccctgtcggg cgtggccctg ttcggcctgc tcgttggagg
     1261 cgcaacctcc gcgcgcgcgc aatcgctgcg cccgaacatc atgttccttt tcgacacgtc
     1321 cggctcgatg cacgaggact cgaacctcgt cgatagagcc gacggcacga cggtgtgccc
     1381 gcagagcacg accagccgca tctacagcct gaagagcggc atccgggcgg cgctgcagca
     1441 ggtcggcacg gacgaggcga acttcggcct catgtcgttc ccgcagacgg tttcgaacac
     1501 ctacacggcg ctcacggcca atcagtgcac caatacgggg caaaccccga tcggccacta
     1561 cgccgccacg ggcacgcaga cgatcaccgt gccgaaccga gtctccacgg ggttccacaa
     1621 cgcgaccagc tactcgattg gttgcctgat gacgaccaac aact
//
LOCUS       DASWVF010000259         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_83243, whole genome shotgun sequence.
ACCESSION   DASWVF010000259 DASWVF010000000
VERSION     DASWVF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83243"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGD55_04615"
     CDS             <1..633
                     /locus_tag="VGD55_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0869657.1"
                     /translation="TAPAPSFQVARPVLSEDQEDAMHCKDIMSTNLEWLTEKDTIADA
                     AKKMADTGLGFLPLCDERMRLIGVVTDRDLTVRALAKGLDVKGTTAAMVMTSPAITCL
                     SSAELGEAERLMAEGRKSRIAIVDPDGALVGVVSLADLVEHAPRSQAWKTLQSVLWRE
                     ALGARGGAPAWQPLLKDDPVARSQPVADEVHRGATVFTGGTHSVDTKEFP"
     gene            675..1745
                     /locus_tag="VGD55_04620"
     CDS             675..1745
                     /locus_tag="VGD55_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0869658.1"
                     /translation="MSTTMRAAVFQGKGKIGLREVPRPVPGIGQALIKVTLTTICGTD
                     VHILKGEYPVREGLVVGHEPVGAIEELGPGVTGYQKGDRVIVGAITPCGQCRACLTGV
                     ASQCGHGGSGSEAIGGWRFGNTIDGCQAEYVLVPNAQANLANIPHGLTDEEVLLCPDI
                     MSTGFSAAERGHVRLGDSVVVFAQGPIGLCATAGARLAGASLVIAVDSVPKRLEFARR
                     MGADVVLNFKEQDVVAEVKRLTDGGADVAIEALGQSQTFENCLRSIRPGGTLSSLGVY
                     SGHLTVPLDAYAAGLGDHTIVSSLCPGGKERMRRLMSIVAANRFPFKDLVTHSFRLAE
                     IEAAYDLFSHQRDGVMKVAIRP"
     gene            complement(1892..2377)
                     /locus_tag="VGD55_04625"
     CDS             complement(1892..2377)
                     /locus_tag="VGD55_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0869659.1"
                     /translation="MFAHDHRIEVRDFSGQATFAKAPSHTAASLQLTVAAKSLFLVGE
                     DSLGARQSIEAALRQDVLETDKFPQIVFKSKTVTSERRGDGTYDVRLQGELSLHGVKR
                     SIVVPARVSVENGSLHAIGVFEIRQTDYKITPFSFAGGTVGIKDTVTLSFDILAGQPQ
                     S"
     gene            complement(2404..>2663)
                     /locus_tag="VGD55_04630"
     CDS             complement(2404..>2663)
                     /locus_tag="VGD55_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869660.1"
                     /translation="PPRQPLRGIRRLTQSLRWSRRHCPDSRLALELHWSGIMKTTALF
                     LLFAPFVAGAAFSAPADDKAPPRSAKDGGVYQIDASHSRSW"
BASE COUNT          471 a          838 c          907 g          447 t
ORIGIN      
        1 accgcacctg cgcccagttt ccaggtggca cggcccgtgc tgtcagagga ccaggaggac
       61 gcgatgcact gcaaagacat catgagcact aacctggaat ggctcaccga gaaggacacg
      121 attgccgacg cggcaaagaa gatggccgac acagggctgg ggttcctgcc cctctgcgac
      181 gaacgaatgc ggctcatcgg cgtcgtcacg gatcgagatc tgacggtgcg cgcgctcgcc
      241 aaaggcctgg acgtcaaggg aacgacggct gcgatggtga tgacctcacc cgcgattacc
      301 tgtctttcgt ccgccgagct gggcgaagca gagcgattga tggccgaggg acggaagtcc
      361 cgcatcgcaa tcgtcgaccc agatggggca ctggtcggcg tggtcagcct ggcagacctg
      421 gtcgagcacg ctccccgctc tcaagcgtgg aagacgttgc agtcggtgct ctggcgagag
      481 gcgctcggtg cgcgcggagg cgcaccggcc tggcagccgc tgctgaaaga cgatccggtc
      541 gcccgctctc agcctgtcgc cgatgaggtg caccggggcg cgaccgtgtt caccggggga
      601 acgcactcgg tcgatacgaa ggaatttccg taaagacacg ctggccccag tcgagatcag
      661 aaaggaaacg acacatgagc acgacaatgc gagcggcggt gtttcagggc aaagggaaga
      721 tcgggcttcg cgaggtcccg cggcccgtcc ctggtatcgg ccaggccctg atcaaggtga
      781 ccctcacgac gatctgcgga acagacgtcc acatcctgaa gggcgaatat ccagttcgcg
      841 agggtctggt tgtcggccac gagcccgtcg gcgcgatcga ggagctcggc ccgggtgtca
      901 ccggctacca gaagggcgac cgggtcatcg tcggcgccat cacaccatgc ggccagtgcc
      961 gtgcctgcct gacaggcgtc gcgtcccagt gcgggcacgg cggttccggc tcggaggcca
     1021 tcggcggatg gcggtttggg aacaccattg acggctgcca ggcggagtac gtcctggttc
     1081 ctaacgccca ggcaaacctc gccaacatcc cgcacgggct gaccgatgag gaggtgcttc
     1141 tctgccctga catcatgtcg acaggcttct ctgcggcgga acgcggccat gtccgcctcg
     1201 gcgactcggt ggtggtgttc gctcaagggc ccatcggcct ttgcgcgacg gcgggcgcgc
     1261 ggttggccgg cgcgtcgctg gtcatcgccg tcgacagcgt tcccaagcgc ctcgagttcg
     1321 cccggcgcat gggagctgac gtcgttctga acttcaaaga gcaggacgtc gtcgccgaag
     1381 tgaagcgcct gacggacggc ggtgccgacg tcgcgatcga ggcgctcggt cagtcgcaga
     1441 cgtttgagaa ttgcctccgc tccattcgtc ccggcgggac gttgtcgagc ctcggggtct
     1501 actccggcca cctgaccgtt ccgctggacg cctacgcagc gggattgggc gatcacacca
     1561 tcgtgagctc gctctgccct ggcgggaagg agcgcatgcg ccggctgatg agcatcgtgg
     1621 ccgcgaaccg gttcccgttc aaggatctcg tgacgcactc gtttcgactg gcggagatcg
     1681 aagccgcgta tgatctgttt tcgcaccagc gcgacggcgt catgaaagtc gccatccggc
     1741 cgtaaccggg accctgccgc cgaccaatcg accgccgtca cgcgcgagct ctcgggcggt
     1801 cggtggcgca atcgccagct cggtcagccg gccgcgcagg cgttcgtgct cgtccagcag
     1861 cgcgtgagca gcacccgcgc ctatagacgt gtcaggactg cggctgcccg gccaggatgt
     1921 cgaacgagag cgtcacggtg tccttgatgc cgacggtgcc gccggcgaac gagaacggcg
     1981 tgattttgta gtccgtctgg cggatctcga acacaccgat ggcgtgaagg gatccgttct
     2041 cgacggacac gcgcgcagga acgacgatcg accgtttgac gccgtgaaga ctgagctcgc
     2101 cctgaaggcg cacgtcgtac gtgccgtcgc cgcgccgttc cgacgtgacc gtcttgctct
     2161 tgaagacgat ctgggggaat ttgtcggtct ccagcacgtc ctggcgcagc gctgcttcga
     2221 tcgactgccg cgcgcccaag ctgtcttcgc cgaccaagaa cagcgacttc gccgccacgg
     2281 tgagctgcag cgacgcggcg gtgtgagacg gggcctttgc gaaggtcgcc tggccactga
     2341 agtcgcgcac ttcgatgcgg tggtcgtgtg cgaacatcgc ggacaggccg gtggtctcgg
     2401 tctttaccag gaacggctgt gggaagcgtc gatttgatac accccgccgt ccttggctga
     2461 acgaggtggc gccttgtcgt cagcgggggc gctgaacgcg gcgcccgcga cgaatggagc
     2521 gaagaggagg aacaaggcgg tcgttttcat gatccccgac caatgcagct cgagtgccag
     2581 ccgcgagtct gggcaatggc gtctcgacca gcgcaatgat tgcgtcagac gacgaatgcc
     2641 acgcaacggc tgccgcgggg gca
//
LOCUS       DASWVF010000260         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_287267, whole genome shotgun sequence.
ACCESSION   DASWVF010000260 DASWVF010000000
VERSION     DASWVF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287267"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGD55_04635"
     CDS             complement(<1..225)
                     /locus_tag="VGD55_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEY0869661.1"
                     /translation="MIKQVIGVLGGTGLQGGGVVDALLAQGTFEVRVASRTPSSEKAR
                     GLAARGVEVVKGDLLEPASLDAFFDGSHGAF"
     gene            complement(235..1170)
                     /locus_tag="VGD55_04640"
     CDS             complement(235..1170)
                     /locus_tag="VGD55_04640"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0869662.1"
                     /translation="MSGSRGSAPRRSRRPPSGARRARPSACQYRARSRTAAWFITGAS
                     SGIGAALVTAALDAGDHVVATARNVEKLRSAIGQPASERLAFVPLDVTNEERAHEAIT
                     VAVERFGRIDVLVNNAGYCLMGDLESLTTKQIEQQFATNFYGVLYVMRAALPVMRRQG
                     SGRIFNVTSMAGVVGYGKCSAYAASKFAVEGLSLSVAQEVERFGITLTCVEPGFFRTD
                     LLAPQSSVFGDIAVEGYDSPAEIKAKWLSYHGKQGGDPAKLGKALVQLAAAPSPPKQF
                     YAGTDAVDGITADLKARLDDAQAHRDLSVTTDGNF"
     gene            complement(1908..1981)
                     /locus_tag="VGD55_04645"
     tRNA            complement(1908..1981)
                     /locus_tag="VGD55_04645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1945..1947),aa:Pro,seq:cgg)
BASE COUNT          365 a          722 c          664 g          344 t
ORIGIN      
        1 gaacgcgcca tgggagccgt cgaagaaggc atcgaggctc gcgggctcga gcaagtcacc
       61 cttcacaacc tcgactccgc gcgcagcgag cccccttgct ttctcgctgg agggcgtgcg
      121 actcgcgact cgtacctcga acgtgccctg ggcaagcagt gcgtcgacca cgccgccacc
      181 ctgaaggccg gtaccaccga gcacgccgat cacttgcttg atcattttcg tctcctagaa
      241 gttgccgtcc gtcgtgacgg acaagtccct gtgcgcctgc gcgtcgtcaa gccgcgcctt
      301 gaggtcggcg gtgatgccgt cgaccgcatc ggtccctgcg tagaactgct tcggtggcga
      361 cggtgccgct gcgagctgaa cgagcgcctt gccgagcttc gcgggatctc ccccctgttt
      421 gccgtggtag ctgagccact tcgccttgat ctctgctggc gagtcatagc cctcgaccgc
      481 gatatcgccg aacacggagg actgtggcgc gagcagatcg gtgcggaaga agccgggctc
      541 gacacacgtg agcgtgatgc cgaatcgctc gacttcctga gcgaccgaca gcgatagccc
      601 ctccaccgca aacttcgacg cggcgtaggc gctgcacttc ccatagccga cgacgccggc
      661 catcgaggtc acgttgaaga tgcgccccga gccttgccgc cgcatgacag gtagcgccgc
      721 gcgcatcacg tacaggacgc cgtagaaatt cgtcgcaaac tgctgctcga tctgtttcgt
      781 ggtcagggac tcgagatcgc ccatgaggca atagccagcg ttgttgacca gaacatcgat
      841 gcgtccgaac cgttcgacgg caacggtgat ggcctcgtgg gcccgctctt cattcgtgac
      901 gtcgagcgga acgaacgcca gacgttcact tgctggctgg ccgatcgcgg accgcagctt
      961 ctccacgttt cgcgccgtgg cgacgacgtg atctcccgcg tccagcgcgg ccgtgaccag
     1021 cgccgcgccg atgccactgc tcgcgcccgt gatgaaccaa gccgctgttc gcgaccgcgc
     1081 tctatactgg catgcggaag ggcgagctct tcgcgctcca gaaggcggac gtcgacttcg
     1141 acgcggggct gatcctcgtg agccgctcac acgatcggga catcccgaag ggagggcggg
     1201 tcgaggcggt cccgatcaac agcgagctga ctgcgtacct taaaaaggca atcgcggcgt
     1261 caccctcgtc gctggtgttc ccagacgaga gcggggagat gctgcccaag cacacagcga
     1321 tggaggaggt cctccggcgc gcgatgcgcc gtgcgggcat cgtgacgggc tacgtccata
     1381 agtgccggcg gaagggctgc ggccaccacg acccggcgcc cgacgcgaac ccgcgccgct
     1441 gcccggtctg ccagttcaag ctctgcccgg tcggcgaggt acgaaagctg cgctttcacc
     1501 acctgcgcca cacgaccgcg tcgctcctac tcatgcaggg cgccgacctg gccgctgtcc
     1561 agcggatcat gcgccaccag gacctgcgca tcacgacgga ggtctacggg cacctcgccc
     1621 ccggctacct ccagaaggag atcaaccgcc tccggttcga gccgacgtcc gtggaccagg
     1681 gggcgaccgt catcccgatc agcgcggcag cgaccgccag cggtccctca ggctcggatg
     1741 agccggttca tgcgtcggcg ggtggcgcct cggagccgcc gctgcccgcc gatcttcaga
     1801 ccgacgcggc tccgttttct acccggtttc tacccaaccc ccagatcgcc catgcaccct
     1861 cgacgcgacg cctcggaatc tccaagcaac gtcgcggagt taagttgtcg gggcgagagg
     1921 atttgaacct ccgacctttc ggtcccgaac cgaacgcgct accagactgc gccacgcccc
     1981 gatatttgtg tcggggcttc atgccaaact tagcgccgag agtcaatggg ggcgggcggc
     2041 gaggcggaga cggcggaggg cgacgtcggc gatgtacagc agcacggcgg ccagc
//
LOCUS       DASWVF010000261         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_159841, whole genome shotgun sequence.
ACCESSION   DASWVF010000261 DASWVF010000000
VERSION     DASWVF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159841"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1367
                     /gene="prfB"
                     /locus_tag="VGD55_04650"
     CDS             264..1367
                     /gene="prfB"
                     /locus_tag="VGD55_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0869663.1"
                     /translation="MVTADPREELKALDVTLSSIEQVLDVPGLRRQVVQLEEQAAAPD
                     LWNDPDAAQRVTSKLSHAQGDVRRIEGLRSRLDDVGVLLDLAASEDDAGVHTEALAEI
                     ASLTKAIGELEVRTLLSGEYDSREAVVTINSQAGGVDAADWAQMLLRMYLRWAERHNY
                     PTEVYDTSYAEEAGIKSATFAVKVPYAYGTLSPEHGTHRLVRISPFDNQGRRQTSFAG
                     VDVVPVVESSDHVEIPDDDIRVDVYRSSGPGGQGVNTTDSAVRITHLPTGIVVSCQNE
                     RSQIQNRASAMLVLQAKLLERRRQDEAAKMAEIRGDSTTSWGTQIRNYVLHPYQLVKD
                     LRTDVETGNTTAVLDGEIDEFIEAGIRWRRQHS"
     gene            1705..>2803
                     /locus_tag="VGD55_04655"
     CDS             1705..>2803
                     /locus_tag="VGD55_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869664.1"
                     /translation="MHLHPRIPRPRSIAAVAISIGLGSVASFALVTDASAHANIVHGT
                     SVCNQTDGTYTITWTIANDYNLSDTVSKVSDTGGGTLSGLPATIAASPHTPYNTATAT
                     QTGVPGTATTATLTINGLWSDGYHQQDGASVTLGGNCVIPPTRTTTAAATFTDATCAA
                     ATGSYTIPSSTSADYFVAINGGASAAAAAGTVSEPAGTAIVVTAHAKTGFVLTGTTNW
                     SHTIAAATGCTTSPPPSSPPPSSPPPVVHPATIAPAVPTITQGVCVSGKQTSAFFTIP
                     GITGVTYLANGVSVAAGNHNVAYGSTTTVTAAALAGYSFPAGTTTSWTLVANAALPSC
                     AAAPTAVLGITFNNPPATPPVKSPPSAVLPFT"
BASE COUNT          529 a          940 c          869 g          465 t
ORIGIN      
        1 gctcgggacc ggtgcagtac tgacgatcgc tggtgccgac gagctcggcg ttacctcggc
       61 ttgcgacgcc ggggccgacg acacctggct cgcagcgacg tgatgcgacg tcgaacaggc
      121 gcccaacatc aaggccgcgg cgccgatgac cgcgggaacc ctgatgcagc ggggcaggaa
      181 cgaaggcacc cctcatcttc gcgcatcggg cagtcgcggc tcaccgattc ggcgcctacg
      241 cggcagcgac tagagtcctt cgggtggtaa ccgcagatcc ccgcgaggag ctcaaggcac
      301 tcgacgtcac cttgtcgagc atcgagcagg tgcttgacgt gcccggattg cggcggcaag
      361 tggtccaact cgaagagcag gcggccgctc ccgacctctg gaacgatccc gacgccgcgc
      421 agcgggtgac gagcaagctt tcccatgccc agggcgacgt tcggcgcatc gaggggctcc
      481 gatcgcggct cgacgatgtc ggcgtcctgc ttgatctggc cgcgtcggaa gatgacgcgg
      541 gcgtgcatac cgaagccttg gcggagatcg cttcgttgac gaaggcaatc ggcgaactcg
      601 aggttcgcac cctgttgtcg ggggagtacg actcccgcga ggccgtcgtc accatcaact
      661 cacaagcagg cggggtcgac gccgccgact gggcgcagat gttgttgcgc atgtacttgc
      721 gctgggccga gcggcacaac tacccgaccg aggtttacga cacctcctac gccgaagaag
      781 ccggtatcaa gtcggcgacc tttgcggtca aggttcctta cgcatacggc actttgtcgc
      841 ccgagcacgg cacccaccgc ctcgtgcgga tcagtccgtt cgacaatcaa ggccgtcgcc
      901 agacctcgtt cgccggcgtc gacgtcgtgc ctgtcgtcga atcttctgat cacgtcgaga
      961 ttcccgacga cgacatcagg gttgacgtat accgctcatc tggcccgggt gggcaaggcg
     1021 tgaacacgac cgactctgcg gtgcgcatca cgcacttgcc gacggggatc gtcgtgagct
     1081 gccagaacga acgcagccag atccagaacc gcgcgagcgc aatgctggtg ctgcaagcga
     1141 aattgctcga gcggcgccga caggacgaag ccgcgaagat ggcagagata cgcggcgaca
     1201 gcaccactag ttggggaact caaatccgta actacgtgtt gcatccctac caactcgtga
     1261 aagaccttcg caccgatgtc gagaccggca atacgaccgc ggtgctcgac ggtgagatcg
     1321 acgagttcat cgaagccggg atccgctggc gacgccaaca ctcgtagcca cagcaaaacg
     1381 agcatcaggc cggacgccgt tcagcgggcg acctgttgac ccatcaggag tgatcgtcga
     1441 accgaattca ctgctccgga tggcgtaacc atcgttactc tgcgtggcgt aacttcggtc
     1501 aaattcgacc ccagatcggc aagttgcgtt gcgaagcctt tgcgatgtgg caatgatgtg
     1561 cccgtagcgg ttttgcaaga aatcgtgggg acgcattgat ccgcgactgg aaacacggtc
     1621 acctgccgta gctcatacgg atggttgcgg ggagcgagtg gtgagaccga ccccttgtcg
     1681 tttcgactag agggtaggtc tgtcatgcat cttcatccac gaatcccacg gccgcgttcg
     1741 atcgcggcag tcgccatcag cattggtctc ggcagcgtgg cgtcgttcgc gctcgtcacc
     1801 gacgccagtg ctcacgcgaa catcgtgcac ggcacgtcgg tctgcaacca gaccgacggc
     1861 acctacacga tcacctggac catcgcgaac gactacaacc tgagcgacac cgtcagcaag
     1921 gtgtcagaca ccggcggcgg cacgttgtca gggctgccgg ccacgatcgc cgcgtcgccg
     1981 cacacgccgt acaacacggc gaccgcgacc cagacgggtg tgcctggcac ggccacgact
     2041 gccacgttga ccatcaacgg gctctggtct gatgggtacc accagcagga cggcgcctcg
     2101 gtcacgctcg gcggtaactg cgtcattccg ccgacccgca ccactactgc cgctgcgact
     2161 ttcactgacg cgacgtgtgc agctgcgacc ggcagctaca cgattccgtc atcgacgtcg
     2221 gctgactact tcgttgcgat caacggtggc gcaagcgccg ctgctgcagc cggcaccgtg
     2281 agcgagcctg cgggcactgc aatcgtcgtg acggcacacg cgaagaccgg cttcgtgctt
     2341 acgggcacga caaactggtc gcacaccatc gcggcagcca ctggctgcac cacgagcccg
     2401 ccgccgtcca gcccgccgcc ctcgagcccg ccgcccgtcg tgcaccccgc gacgatcgcg
     2461 cccgctgtgc cgacgatcac gcaaggcgtg tgtgtctccg gaaagcagac cagcgcgttc
     2521 ttcacgatcc ccggcatcac cggcgtcacc tacctcgcca acggcgtctc ggtagcggcc
     2581 ggtaaccaca acgtggcata cggctcgacg acgacggtga cggccgccgc tctcgccggg
     2641 tactcgttcc cggccgggac gacgacgtcg tggacgttgg tcgccaatgc tgctctgccg
     2701 tcgtgcgcag cggcaccgac cgccgtcctc ggcattacgt tcaacaaccc gccggctacg
     2761 ccgccggtga agtcgcctcc gagtgcagtg cttccgttca cgg
//
LOCUS       DASWVF010000262         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_287273, whole genome shotgun sequence.
ACCESSION   DASWVF010000262 DASWVF010000000
VERSION     DASWVF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287273"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="hflK"
                     /locus_tag="VGD55_04660"
     CDS             complement(<1..719)
                     /gene="hflK"
                     /locus_tag="VGD55_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0869665.1"
                     /translation="MVLVALVGSYYQVEPDEVGLVTRFGRFVRTTNPGPHGKIPFGIE
                     QVQKVPVQRQLKQEFGFRTVSAAVDSTFRKDEKTAAESLMLTGDLNVATVEWIVQYKI
                     SDPYKYLFRLRDVEETFRLMAEASMRTVVGDHSVTELLTVGREAIAAKAKELLADLCK
                     RYDNGISVQQLVLQDVDPPESVKPSFNAVNQAIQERERAINEAWAEYNQEIPRARGLA
                     EQKIEASEGYAVDRVNRAKGDA"
     gene            complement(918..1784)
                     /locus_tag="VGD55_04665"
     CDS             complement(918..1784)
                     /locus_tag="VGD55_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869666.1"
                     /translation="MKELFPDIVLPISTRPGVDVCVAAGASPNELLWLTAPKRAAHDR
                     VFPPDAAVYLHSFRAGPEITTRPYKPRRRPHFIQRIGADVLFVAGRQRDRRPNGWLLD
                     GAGRVVRRVPLGDGLSDVRVSPAGKIWTAYFDEGVYGAGDGAEGVVARDGLGRRVWGF
                     DPNRAGTDVIDDVYAFNLAAEDDAWVYFYMPFALVRWRKNKPTVWQTRVEGARAIAVR
                     GTEALLLGDYDDPTVLRILDLPKGGGRARVKRRLRLRLPEGTDVRWLVALGAAHRLAL
                     WSNRDLMILDRW"
     gene            complement(1794..>2246)
                     /locus_tag="VGD55_04670"
     CDS             complement(1794..>2246)
                     /locus_tag="VGD55_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869667.1"
                     /translation="GYGVTMMKVGALWVYNHSGGVPNFSAFVAWVPAKRIGAAAMMNA
                     TNVAGATPGSVVLRALSVLVDLPAEWRATAEGPHRPLAAYAGTYVDRMSWLGRLRVRV
                     EGDRLAFDYLDGPPALLPPTFVFRFVPGEVHARFVVTPVGVGERVANE"
BASE COUNT          394 a          772 c          716 g          364 t
ORIGIN      
        1 gcatccccct tcgcccggtt cacccggtcg accgcatagc cctcggaggc ctcgatcttt
       61 tgttcggcca ggccgcgggc gcgcgggatc tcctgattgt attcggccca ggcctcattg
      121 atggcgcgct cgcgttcctg gattgcctgg ttcacggcgt tgaatgaagg cttcaccgac
      181 tcgggcggat cgacgtcctg cagcaccagt tgttgcacgg agatcccgtt gtcgtaccgc
      241 ttgcacaggt cggcgagcag ctccttggct ttggcggcga tcgcctcacg cccgacggtg
      301 agcaactccg tgacgctatg gtcgccgacg acggttcgca tcgacgcctc ggccatgagg
      361 cgaaacgtct cctccacgtc gcgtagccga aagaggtact tgtacgggtc ggagatcttg
      421 tactggacga tccattccac cgtcgccacg ttcaggtcgc ccgtcagcat cagtgactca
      481 gcggctgtct tctcgtcctt gcgaaacgtg ctgtcgaccg ccgcgctgac ggttcgaaag
      541 ccgaactcct gtttcagctg ccgctgaacg ggcacctttt gcacctgctc gatgccgaac
      601 ggaatcttgc cgtgcggtcc ggggttggtg gtgcgcacga accgcccgaa ccgggtcacg
      661 agaccgacct cgtcaggctc gacctgatag tacgagccga ccagcgcgac gaggaccacc
      721 acgatcccga cagcgccggc gatgagccgt cgcgaccccc gcagccggtc ccaaaaggca
      781 cgcgccgcgc gccgcagctg ctcttgatcg aagtcagacc gaccgttcgt catgggacct
      841 tctttcagag tcccgcacga acatcaagga aggcccctcg gttgccaaca ggcgaacctg
      901 acgcgatcgg acaacatcta ccagcggtcc aggatcatca ggtcccggtt ggaccacagc
      961 gcgagtcggt gtgccgcgcc gagcgcgacg agccagcgca cgtccgtccc ctcggggagg
     1021 cgcagtcgga gccggcgttt gacacgcgcg cggccgcccc cctttggcaa gtcgaggatc
     1081 cggagcaccg tcggatcgtc gtagtctccg aggagcagcg cctccgttcc gcggacggcg
     1141 atcgcgcgtg cgccctcgac gcgtgtctgc cagacggttg gcttgttctt gcgccaccga
     1201 acgagcgcga acggcatgta gaagtacacc cacgcgtcgt cctccgcggc cagattgaac
     1261 gcgtacacgt cgtcgatgac gtcggtacct gccctgttag gatcgaaccc ccacacccgt
     1321 cgaccgagcc cgtcgcgggc gacgacgccc tcggcgccat cgccggcacc gtatacgccc
     1381 tcgtcgaaat aggcggtcca gatctttcca gcaggcgaga cccggacatc cgacagcccg
     1441 tcgcccagcg gcacgcgacg cacgacgcgt ccggcgccgt cgagcaacca gccgtttgga
     1501 cgtcgatcgc gctggcgacc ggcgacgaac agaacgtcgg cgccgatgcg ctgaatgaag
     1561 tgcggacgcc ggcgtggttt gtacggtcgg gtggtgatct ccgggcccgc gcgaaacgag
     1621 tgcaggtaga ccgccgcgtc cggtgggaag acccggtcgt gggccgcgcg cttgggcgcg
     1681 gtcaaccaca ggagctcatt gggggatgcg ccggcagcca cgcacacgtc gactccggga
     1741 cgcgtcgaga tgggcagcac gatgtcagga aacagctctt tcatcggttg agctcactcg
     1801 ttcgcgacac gttcgccgac tcccaccggt gtgaccacga accgcgcatg cacctcgccc
     1861 ggtacgaagc ggaacacgaa cgtcggcggc aacagcgccg gcgggccgtc gagataatcg
     1921 aaggcgagcc ggtcgccttc gacgcggacc cggagccggc caagccagct catgcgatcg
     1981 acgtaagtgc cggcgtacgc ggccaacgga cgatgggggc cctcggccgt ggctcgccac
     2041 tcggcgggca gatctacaag cacgctcagc gcacgaagaa cgacggaacc aggggtggct
     2101 cccgcaacgt tcgtcgcgtt catcatcgcg gcggcgccga tccgtttcgc gggaacccag
     2161 gccacgaacg cgctgaagtt tggaacgcca ccactgtggt tgtacaccca gagggcgcca
     2221 accttcatca tcgtgacgcc gtagcc
//
LOCUS       DASWVF010000263         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_236473, whole genome shotgun sequence.
ACCESSION   DASWVF010000263 DASWVF010000000
VERSION     DASWVF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236473"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..251)
                     /locus_tag="VGD55_04675"
     CDS             complement(39..251)
                     /locus_tag="VGD55_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY0869668.1"
                     /translation="MSDRTYRVTEVVGTSKESVQHAVRNGVRRASETLRHLDWFEVTE
                     IRGHIVDGDVEHFQVSMKVGFRLEDV"
     gene            311..658
                     /locus_tag="VGD55_04680"
     CDS             311..658
                     /locus_tag="VGD55_04680"
                     /inference="COORDINATES: protein motif:HMM:NF022742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEY0869669.1"
                     /translation="MARQVRGGGHEDDPQVYLVTTARPGHSQEMSGRLRRYLISMTIR
                     VVFLVLAIFVFSGWLRVVGIAAAVVLPWIAVVMANAGPTGDDEQPTFVSTDHIAIDGP
                     PATQIGQSGEGKA"
     gene            655..1680
                     /gene="nadA"
                     /locus_tag="VGD55_04685"
     CDS             655..1680
                     /gene="nadA"
                     /locus_tag="VGD55_04685"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0869670.1"
                     /translation="MTADLYPLPPVPPEPVLPPPGVPEGWAEEIRSLAAARNAVILAH
                     NYMLPEIQDIADHVGDSLALSRIAAASDADTIVFCGVHFMAETAKILAPSKTVLIPDL
                     DAGCSLAASIDVSDVLAWKAGHPDGVVVAYVNTSAAIKAEVDVCCTSSNAADVVRSIP
                     QDKEIFFLPDQFLGAHVRRVTGRDMDVWAGECHVHAEISPSDLRKTLEANPDADLLVH
                     PECGCTTTALYLTSTGDLPADRTRVLSTGGMVEYAKNTTAARVLVATETGILHQLHKL
                     NSTTDFMPVSPRAVCPYMKMITPAKLLRSLRENLFEVDVDPVTAKRARSAVERMIEIG
                     SPSRGGE"
     gene            complement(1686..2009)
                     /locus_tag="VGD55_04690"
     CDS             complement(1686..2009)
                     /locus_tag="VGD55_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0869671.1"
                     /translation="MLLVCVDPSEPTPDDPGGLTIEEWLEETQRRGVRLDGDRLQPPK
                     AATTVRSRAGRVVVTDGPFVETKEVMAGYDIIAAANLDEAIEIASKHPVARIGMIEIR
                     PFWVE"
     gene            complement(2116..2469)
                     /locus_tag="VGD55_04695"
     CDS             complement(2116..2469)
                     /locus_tag="VGD55_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869672.1"
                     /translation="MRRLLAGSVFACLVAGLPAIMLTGCRQTASLSKQEVVVAFTLTA
                     TPADHARVWALCQHVDGTTPEPLVTKSKYPATLRSNVRFRVDHATNLQLQQLFDCLKQ
                     DPSVVGYSTTGDGDQ"
     gene            complement(2474..>2730)
                     /locus_tag="VGD55_04700"
     CDS             complement(2474..>2730)
                     /locus_tag="VGD55_04700"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0869673.1"
                     /translation="FWMLFLEKSPWQTLTLIVSLEAIFLSTFVMIGQNRQSSFAAAKA
                     NHDFVAQETELKQNTDLTRQIHTLTTEIHTKLAESNGPVA"
BASE COUNT          482 a          879 c          847 g          522 t
ORIGIN      
        1 gacttcaaca tgagcacggt gagccaggct tcctgacctc agacgtcttc gagccggaaa
       61 ccgaccttca tgctgacctg aaaatgctcc acgtcaccat ccacgatgtg ccctcgaatc
      121 tcggtcacct cgaaccagtc aaggtggcgc aaggtctccg atgcccggcg cactccgttg
      181 cgcacggcat gctgcacgct ttccttcgac gtcccgacga cttcggtgac tcggtaggta
      241 cggtcgctca tgtgttcctc ctgctcgatg tcgcgatgta gagcccatca tcgcggcgta
      301 gcctggggag gtggcaagac aggtacgagg cggcgggcac gaggacgatc cgcaggtcta
      361 tctggtcacc actgcccgcc cgggtcactc ccaggaaatg tcgggacggc tacggcgtta
      421 tctgatatcg atgacgatcc gggtggtgtt cctcgtactg gcgattttcg tgttcagcgg
      481 atggctacgc gtggtcggca tcgcagccgc cgtcgtcctg ccgtggatcg ccgtcgtgat
      541 ggcgaacgcg gggcccaccg gtgacgacga gcagccgaca ttcgtgtcga cggatcacat
      601 cgcgatcgac ggcccgcccg ccacacagat tggccagtca ggggaaggta aggcgtgacg
      661 gcagacctgt atccgctacc tccggtgcca cccgagccgg tgctaccgcc gccgggtgta
      721 cccgagggat gggctgagga gatccggtcg ctcgcggcgg ctcgcaatgc cgtgatcctc
      781 gcgcacaact acatgctgcc cgagatccag gatatcgccg accacgtcgg tgactcgctc
      841 gcgctgtctc gcatcgcggc cgcgagcgac gccgacacca tcgtgttctg cggcgtccat
      901 ttcatggccg agacggcgaa aatcctcgcg ccgagcaaga ccgtgctgat tcctgacctc
      961 gacgccggct gctcgttggc ggcgagcatc gacgtctccg acgtccttgc ctggaaagcc
     1021 ggacatcccg acggtgtggt cgtggcatac gtgaacacct ccgccgcgat caaggccgag
     1081 gtcgacgtgt gttgcacctc gtcgaatgca gccgatgtcg tgcgttcgat accgcaggac
     1141 aaggaaatct tcttcctgcc cgatcagttt cttggcgcgc acgtgcgtcg ggtgacgggt
     1201 cgcgacatgg atgtgtgggc cggtgagtgt catgttcacg ccgagatctc gccgtccgat
     1261 cttcggaaaa cgcttgaagc gaatccggac gccgatctgc tggtgcatcc cgaatgcggc
     1321 tgcacgacca ccgcgctgta tttgacctcg acgggtgatc tgccggccga ccgcacccgc
     1381 gtcttgtcaa ccggcggcat ggtcgagtac gccaagaaca cgaccgcagc gcgggtgctc
     1441 gtggcgaccg aaaccggcat cttgcatcag ctgcacaagc tcaacagcac gaccgacttc
     1501 atgccggtct caccacgtgc cgtgtgcccc tacatgaaga tgatcacgcc ggccaagctg
     1561 ctgcggtcgc tacgcgagaa cctgttcgag gtcgacgtcg atccagtgac cgcgaagcgc
     1621 gcacgctcgg cggtcgaacg gatgatcgag atcggctcgc cctcaagggg tggcgagtag
     1681 gccgctcact cgacccagaa cggcctgatc tcgatcatcc cgatgcgggc gacagggtgt
     1741 ttcgacgcga tctcgatggc ctcgtcgagg ttcgcggcgg cgatgatgtc gtagccggcc
     1801 atgacctcct tggtctcgac gaatggacca tcggtcacta cgacgcggcc ggccctcgag
     1861 cgcacggtcg ttgccgcttt gggtggttgc agccgatcac catcgagccg caccccgcgg
     1921 cgctgcgtct cctcgagcca ctcctcgatc gtcagtccac ctggatcgtc cggcgtcggc
     1981 tccgacgggt cgacacacac caacaacatg tacttcacgg ctctccttta tgactgatgc
     2041 gctcccgctg tcgcggctac cacctcgacg acgaatgccg caggttgggt tcgacatttc
     2101 tgcactgggg caatctcact gatcgccgtc gccggtagtg gagtacccca cgacgctcgg
     2161 atcctgcttt aagcaatcga acaactgctg gagctgaagg ttggtcgcgt ggtcgacgcg
     2221 gaaccgcacg ttcgagcgca gcgttgctgg gtacttgctc ttcgtgacca gcggctccgg
     2281 tgtcgtgccg tcgacgtgct ggcacaacgc ccagacgcgg gcgtgatcag ccggggttgc
     2341 ggtcaacgtg aacgcgacca cgacctcctg cttcgaaagg ctcgcggtct ggcggcagcc
     2401 ggtcagcatg atcgccggca agcccgcaac aagacacgcg aacacggatc cggcgagcaa
     2461 gcggcgcatg ccgtcaggct accggcccgt tactttcggc cagcttcgtg tgaatctccg
     2521 tcgtcaacgt gtggatttgc cgcgtcaggt cggtgttttg tttgagctcg gtctcctgcg
     2581 cgacgaagtc gtggttggcc ttcgcggctg cgaacgacga ctgacgattt tggccgatca
     2641 tgacgaacgt cgacaagaag atggcctcga gactgacgat gagcgtcaat gtctgccagg
     2701 ggctcttctc caggaacagc atccagaacc
//
LOCUS       DASWVF010000264         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_363646, whole genome shotgun sequence.
ACCESSION   DASWVF010000264 DASWVF010000000
VERSION     DASWVF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363646"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1040
                     /locus_tag="VGD55_04705"
     CDS             18..1040
                     /locus_tag="VGD55_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0869674.1"
                     /translation="MRPYDAVLIVSFGGPERPADVMPFLENVTAGRGVPRERLEEVAQ
                     HYYAVGGVSPINGQNRALLAAVKADLAEHGIEVPVYWGNRHWTPTLTEVLAQMRDDGV
                     TNAIAFLTSAYASYSSCRQYLDAIERARTEVGEGAPAVDRIRQYFNHPGFIEPMIGGV
                     EAALRELPAEYRDDAHLAFVAHSIPAAMEATSGPGGHAYTTQLDEAMRLVTAGVEGQH
                     SATLSFCSRSGSPSVPWLEPEIDARLENLATAGARAVVVVPIGFVSDHMEVVYDLDTD
                     AAGVAGKLGLPFVRSATVGTAPPFVAMVRELVLERMGEGAPRAVGDFGPAHDRCPIDC
                     CRLPAR"
     gene            1076..1879
                     /locus_tag="VGD55_04710"
     CDS             1076..1879
                     /locus_tag="VGD55_04710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0869675.1"
                     /translation="MADESTAQLLELAVRAARLAGEFLIESRPVDLVVRTKSTPTDVV
                     TQMDTAAERLIATTIRASRPDDAMLGEEGQADDVLAGRRVRWIVDPIDGTVNYLYGLP
                     QWAVSIAAEVGGVVVAAAVLDPSKDELFTAELGDGGRRNGVPIGVSACAELAQALVGT
                     GFGYDASRRARQAQVLTGVLPLVRDIRRLGAASLDLCALACGRLDGYFERGLNLWDHA
                     AAGLIAAEAGARVEGLRGRPPDGDLLVAATPGVFDALHDLVAELGADHD"
     gene            2412..>2485
                     /locus_tag="VGD55_04715"
     CDS             2412..>2485
                     /locus_tag="VGD55_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEY0869676.1"
                     /translation="MATDYDTPRKVDEDLGEDSLEELKA"
BASE COUNT          435 a          770 c          865 g          415 t
ORIGIN      
        1 actgcaagga tcgaaagatg cgaccctacg acgccgtgct gatcgtctcg ttcggcggcc
       61 ccgaacggcc agcagacgtg atgccgtttc tcgagaacgt caccgccggg cgcggcgtcc
      121 cccgtgagcg gttggaggag gtcgcgcagc actactacgc ggttggcgga gtaagcccga
      181 tcaacggcca gaatcgtgcc ctgctcgcgg cagtaaaggc tgatttagca gagcacggaa
      241 tcgaggtgcc ggtgtactgg ggaaaccggc attggacgcc gacgttgacc gaagtactcg
      301 cgcagatgcg tgatgacggt gtcacgaacg cgatcgcctt tctgacgtcg gcgtatgcga
      361 gctactcgag ttgccgtcaa tacctcgatg cgatcgagcg ggcgcgaacc gaagtgggcg
      421 agggcgcgcc tgctgtcgac cggattcggc agtacttcaa ccatcccggg tttatcgagc
      481 cgatgatcgg cggcgtcgag gcagcgttgc gcgagttgcc ggcggaatat cgcgacgatg
      541 cacacttggc attcgtcgcc cactcgattc cggccgcgat ggaagcgacg agtgggcctg
      601 gtggacacgc ctacacaacg caactcgacg aggcgatgcg gcttgtcacc gcgggcgttg
      661 aagggcagca ctcggcgacg ctgtcgtttt gtagccgcag cggttcgccg tcggtcccgt
      721 ggctcgagcc tgagatcgac gcgcgcctcg aaaaccttgc taccgcaggg gcgcgtgccg
      781 ttgtcgtcgt tccgatcggc ttcgtctccg accacatgga agttgtctac gacctcgaca
      841 ccgacgccgc cggggtggcg ggaaagctcg gattgccgtt cgtccgatcc gcgaccgtcg
      901 gcacggcgcc gccgtttgtg gcgatggttc gcgagttggt gctcgagcgg atgggcgagg
      961 gcgccccgcg ggcggtcgga gatttcggcc cggctcacga ccggtgcccc atcgattgtt
     1021 gccgactccc cgcccgctga ggccgcccag ttccccgcgt cgatatggtc agcaagtggc
     1081 cgacgaatca accgcgcaac tgctcgaact tgcggtccgc gcggcccgtc ttgccgggga
     1141 gttcttgatc gaatcacggc cggttgacct cgtcgtccgg acgaagtcga ccccgaccga
     1201 cgtcgtgacc cagatggaca ccgcggccga gcgcctgatc gccacgacga tcagagcaag
     1261 taggccggac gacgcgatgc tcggcgaaga gggccaggct gacgatgtct tggccggccg
     1321 gcgagtgcgc tggatcgtcg acccgatcga cggcacggtg aactacctgt acggattgcc
     1381 gcagtgggcc gtctcgatcg cggccgaggt gggcggtgtc gtggtcgcgg ccgctgtgct
     1441 cgaccccagc aaggacgagc tcttcaccgc agaactaggc gatggtggcc ggcgcaacgg
     1501 cgtcccgatt ggagtgtctg cctgtgccga gttggcacaa gccctcgtcg gcaccggctt
     1561 cggatacgac gcgtcgcggc gggcccggca ggcgcaggta ctgactggcg tcctgccgct
     1621 ggtcagggac atccgcaggc tcggcgctgc ttcgttggac ctgtgcgcgc tcgcgtgtgg
     1681 gcgcctggac ggctacttcg aacgtggcct aaatctgtgg gatcacgccg ccgcggggct
     1741 cattgcggcc gaagcgggtg cgcgagttga gggcttgcgc ggccggccac cggacggtga
     1801 tctcctggtt gcagcgaccc ccggggtgtt cgacgccctc cacgatttgg tcgccgaact
     1861 cggcgccgat cacgactagc ccggtctcct agaaactccc ctcaggttcg ccatcgccgc
     1921 ggccgatgag tgtgtgaacc aacgggtgga gaagctcgtt ggggggttgg ctgtggccta
     1981 gctgtgcggc attccggatg cgcgggccgc cgctggaagc atgatcgggc tcgcatggtt
     2041 ggacgctgga gatatgggca taacggacga acctacccgg cagcgggcat cccgcgagcc
     2101 gagaagatga gttggctgtg cttccgtttc gcggaaacga acagcggccc gcgggacaac
     2161 aaaaggcaac gaacggcgga attgtcggcg ctggtcccgc ttcggcgggt cgggcgaccg
     2221 aaggggcggg aaatacggca caatctgcgc tccggaccgg gcacaagttg ggggagttga
     2281 gcgttacact ccgcgccgca gaagaagcaa cagcgagcac tcggatcagc accaccgacg
     2341 agcaccaccg atgagcacca ccagcaccac tgacgagcac gactgaggaa gacgaggcac
     2401 cggaggttcc catggcgacc gattacgaca ccccccgcaa ggttgacgag gaccttggtg
     2461 aagacagtct tgaagagctg aaggc
//
LOCUS       DASWVF010000265         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_7147, whole genome shotgun sequence.
ACCESSION   DASWVF010000265 DASWVF010000000
VERSION     DASWVF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7147"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGD55_04720"
     CDS             complement(<1..415)
                     /locus_tag="VGD55_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEY0869677.1"
                     /translation="MTFGDPIRLDPIHAAWNTVASCGTYEDAQEAVDRLSDSGFPVEN
                     IDIIGSDLRLVERVTGRLTKGGAAAAGAASGAWFGLFIGLLVGLFTSGPVWLGLIVGG
                     LAIGAVWGAVFGFVGHAATGGRRDFASKRTLTATRY"
     gene            complement(447..1868)
                     /locus_tag="VGD55_04725"
     CDS             complement(447..1868)
                     /locus_tag="VGD55_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0869678.1"
                     /translation="MTAPRSAFDAALDRAVVHAKAWLDSMPDRDVAPRMSVDEIGPAF
                     AAPLPEGPTDPAEVIDLLAELAEPGLMAMPGGRFFGWVIGGTLPAALAADWLTSAWDQ
                     NTGLRFATPAAAAIEEAAGGWILELLGLPAGADVGFVTGATMANFTGLAAGRQHVLTK
                     AGWDLDRLGLNGGPRVTVLVGAERHDTIDIALRYLGLGEPTAVEADDQGRIKLDALAE
                     ALDAATGPTIVCLQAGNVHSGAFDPIGPAIELAHAHGEPGDTWVHVDGAFGLWAAASP
                     SLRPLVPGLGNADSWATDAHKTLNVPYDCGLAIVARPEAVRAAFAVHGSYLIAAAGGQ
                     ADPQEKVPELSRRARGIPVWAALRSLGRNGVADLVDGLVKNAQAIAAGISQIEGAQIL
                     NDLVFTQVCVAFEDDAQTRAVTAQLLADGTAWMSGSRWHGKDVLRVSVSNWSTDDADV
                     AASVDAVRRAAQAVRLTSVGGGH"
BASE COUNT          366 a          804 c          663 g          323 t
ORIGIN      
        1 cgtagcgcgt ggccgtcagg gtccgcttcg acgcaaagtc ccggcgtcca cccgtcgcgg
       61 catgcccgac gaagccgaac accgcgcccc agacggcgcc gatcgccaga ccgcccacga
      121 tgagcccgag ccataccgga ccgctagtga ataacccgac gagcaggccg atgaacaggc
      181 cgaaccaggc gccactcgcc gcaccggccg cagccgcgcc acccttggtg aggcgcccgg
      241 tcacccgctc gaccagccgt aggtcggacc cgataatgtc gatgttctcg accgggaacc
      301 cggagtcaga caacctgtcg accgcctctt gggcgtcctc atacgtaccg caactcgcga
      361 cggtgttcca cgctgcgtgg atcgggtcaa gcctgatcgg atctccgaac gtcatgatgc
      421 ctcctccttt ttgtggcacg cgacctctag tgtccgccgc cgacgctcgt gagtcgaacg
      481 gcctgggcag cccgccgcac cgcgtcgacg gacgccgcga cgtcggcgtc atcggtcgac
      541 cagttactga ccgacacccg cagcacatcc tttccgtgcc accgcgagcc cgacatccac
      601 gccgtcccgt ctgcaagcag ttgggcagtg accgcccggg tctgcgcgtc gtcctcgaag
      661 gcaacgcaaa cctgggtgaa cacgagatcg ttgaggatct gcgcgccctc gatctgcgag
      721 attccggcgg cgatcgcctg cgcgttcttg accagcccgt cgacgaggtc agcgacgccg
      781 ttgcgaccga gcgagcgcaa ggccgcccac accggaatcc cgcgagcgcg ccgcgacagc
      841 tccggcacct tctcctgcgg atcggcctgc ccaccggccg ccgctatcaa ataacttcca
      901 tggacggcga aggctgcacg cactgcctcg ggccgggcca cgattgcgag accgcagtca
      961 tacggcacgt tgagcgtctt gtgcgcgtcg gttgcccacg agtcggcgtt acccagacca
     1021 ggaactagtg gccgcaagct cggcgacgcg gctgcccata gtccgaaggc gccatcgacg
     1081 tgcacccacg tgtcgccggg ctcaccgtgc gcatgcgcga gctcgatcgc cggcccgatc
     1141 ggatcgaatg caccggagtg cacgttgccc gcctgcaagc agacgatggt cggcccggtt
     1201 gctgcgtcca acgcttcggc gagtgcgtcg agcttgatcc gaccctggtc gtcggcttcg
     1261 accgcggtcg gctcgcccag gccgaggtag cgcagggcga tgtcgatcgt gtcgtgccgc
     1321 tcggcgccga cgagcaccgt gacgcgaggg ccgccgttca gaccaagccg atcgaggtcc
     1381 cagccggcct tcgtgagcac gtgctggcga ccggcggcga gaccggtgaa gttcgccatc
     1441 gtcgcgccgg tcacgaaccc gacgtccgcc cctgcgggta gacccagcaa ctcaaggatc
     1501 cagccgcccg ctgcctcctc gatcgcagcc gccgccggag tagcgaaccg caaaccggtg
     1561 ttctggtccc acgcgctcgt gagccagtcg gctgcgagcg ccgcgggcag agtgccaccg
     1621 atcacccagc caaagaaccg gccgccgggc atcgccatca ggcccggctc ggccaactca
     1681 gcaagcaggt cgatgacctc cgccggatcc gtcggcccct ccggcaacgg cgctgcgaac
     1741 gccggcccga tctcgtcgac cgacatacgc ggggcgacgt ccctgtcggg catcgagtcg
     1801 agccaagcct tcgcgtgcac aaccgcccga tcgagtgcgg catcaaaagc gctacgtggc
     1861 gcggtcatcg cgcaatcctc atcaacatcc ccccatcatc gttggccccg acgcgcgccg
     1921 tcgacaccct ttcccgactc tttacttcct cggcgcaggc agtcgcactc gggccgctcg
     1981 cgagccggcc cgcaccgtac tcgtcagctc gtctagcccg tcagcgccaa tggcggactg
     2041 aagtcgatcg agccgtcgcc gttcaatctg acctgccaac ccttttcgtg aacgaagcga
     2101 tgatggcgtc ggcacagcaa caccaggttg tcgagcgccg tagcgccgcc gtggat
//
LOCUS       DASWVF010000266         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_185597, whole genome shotgun sequence.
ACCESSION   DASWVF010000266 DASWVF010000000
VERSION     DASWVF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185597"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGD55_04730"
     CDS             <1..808
                     /locus_tag="VGD55_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY0869679.1"
                     /translation="KVWLPSGGYLVIDRTEAMIVIDVNTGRFIGKGGNLEETVTKNNL
                     EAAEEIVRQLRLRDIGGIIVIDFIDMVLEGNRDLVLRRLVECLGRDRTKHQVAEVTSL
                     GLVQMTRKRVGNGLLEAFSEICEVCNGRGVIVHTDLVEHNHAPRQLAVSKPTPAVVAA
                     VRGKGSRGRGAAAGTAAASADADASVVEGGGEPVAEGDEAQTPDGPQAERAEGADAAE
                     LTDGAEVKAPKSAGSGASRSRRGGRRRGGSKANAGADHTAAAHDPGPAGS"
     gene            complement(928..>2044)
                     /locus_tag="VGD55_04735"
     CDS             complement(928..>2044)
                     /locus_tag="VGD55_04735"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0869680.1"
                     /translation="ATSSASPVAKSASPTAPTRPASASPSTSGPPVVQGSDAGLMVLE
                     KTITGHLDEKSVDASGTGLFFAQNMMYLHTINVYNESYQLVKIIPDTVDLAAFGYPQY
                     PGLYKGAPVEAAFTPDHKSVYISNYSMYGAAPFTREGSDTCTLADHYPDSFVYRINVA
                     TLAIDQVIEVGAVPKFVAVTPNGKYLLVSNWCSYNESIIDTATGKVVTTIPLGPYPRG
                     IAIDPVSGTAYVAVMGSTRIAAINLATFAVSWINGVGSAPRHLVMSPDGHWLYATLNG
                     EGDVAKVSLVSRTVAARVRTGDAPRSMTMSPDGDYLYVVNYMSNTMSKVRTSDMYVVQ
                     TVPTNPDPIGITYDAATHHVWVACYSGSIMVFEDGTS"
BASE COUNT          361 a          655 c          680 g          348 t
ORIGIN      
        1 caaggtctgg ttgcccagcg gtggatatct cgtgatcgac cgcaccgagg cgatgatcgt
       61 catcgacgtc aacaccggcc gtttcatcgg caaggggggc aacctcgaag agactgtcac
      121 gaagaacaac ctcgaagcag ccgaagaaat cgtgcgccaa ctgcggttgc gcgacatcgg
      181 cggcatcatc gtgatcgact tcatcgacat ggtgctcgag ggcaatcgcg acctcgtgct
      241 acggcgcctc gtcgagtgtc tggggcgcga tcgcaccaag catcaggtcg ccgaggtcac
      301 gtcgctcggc ctcgtgcaga tgacgcgcaa gcgcgtcgga aacggactgc tcgaagcgtt
      361 ctcagaaatc tgcgaagtct gcaacgggcg cggcgtgatc gtgcacaccg acctggtcga
      421 gcacaaccac gcgccgcgtc agctcgcggt ctcaaagccc acgcccgcgg ttgtcgccgc
      481 ggtgcgtggc aaggggtcgc gcggtcgagg cgcagctgcc gggacggcgg cggcttcggc
      541 cgacgcggac gcctctgtag ttgagggcgg cggcgaaccg gtggccgagg gcgacgaggc
      601 acagaccccc gacggcccgc aggccgaacg tgccgaaggt gccgatgccg ccgaactgac
      661 tgacggcgct gaggtcaagg cgccgaagtc ggccggttcc ggcgcttcac ggtcccgtcg
      721 tggcggacgc cgtcgcggcg gctccaaggc caacgccggc gccgaccaca ccgcagcagc
      781 tcacgacccc ggacccgccg gcagctaacg aaccccagct ccccgccgtc ccgccgactt
      841 cacatcccgg gtggttgtcc gaaacccgaa tttggctcga attcacgacc acgccggatg
      901 tgaagtaggc cagggcggcc gtgagcgcta cgacgtcccg tcttcgaaca ccatgatcga
      961 accgctgtag cacgcgaccc agacgtgatg ggtcgcagcg tcgtaggtga taccgatcgg
     1021 gtcggggttt gtcggcacgg tctgaacgac gtacatgtcg ctcgtgcgca ctttgctcat
     1081 cgtgttcgac atgtagttga cgacgtagag gtagtccccg tccggcgaca tcgtcatgct
     1141 gcggggagca tcgccggtgc gcacccgagc cgccaccgtg cgcgagacga gactgacctt
     1201 cgcgacgtcg ccctcaccgt tcaacgtcgc gtagagccaa tgcccgtcag gactcatcac
     1261 caggtgccgc ggcgctgatc cgacgccgtt gatccagctg accgcgaacg tggccaggtt
     1321 gatcgcggca atgcgcgtgc tgcccatgac ggcgacgtag gccgtgccgc tgaccggatc
     1381 gatcgcgatg ccccgcgggt acggtccgag cgggattgtc gtcacgactt tcccggtagc
     1441 cgtgtcgatg atgctctcgt tgtacgagca ccagttcgac accagcaggt acttgccgtt
     1501 cggcgtgaca gccacgaatt tcggtacggc gccgacctcg atcacctgat cgatcgccag
     1561 cgtcgccacg ttgatccggt acacgaagct gtcaggatag tggtcggcca gcgtgcaggt
     1621 gtcagaccct tcgcgggtga atggcgctgc cccgtacatc gaataattcg agatgtagac
     1681 ggacttgtgg tcaggcgtga acgccgcctc gaccggcgcg cccttgtaca agccggggta
     1741 ctgcgggtat ccgaaagccg cgagatccac ggtgtcgggg atgatcttca cgagttggta
     1801 gctctcgttg tacacgttga tcgtgtgcag atacatcatg ttctgcgcga agaacagccc
     1861 ggtacccgac gcgtcgacgg acttctcgtc gaggtgcccg gtgatggttt tctccagcac
     1921 catcaagccg gcgtcagagc cttgaacgac gggcgggccg gacgtcgagg ggctggcgct
     1981 cgctggtctg gtcggcgccg tcgggctggc ggacttcgcg accgggctgg ctgacgacgt
     2041 ggcg
//
LOCUS       DASWVF010000267         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_109217, whole genome shotgun sequence.
ACCESSION   DASWVF010000267 DASWVF010000000
VERSION     DASWVF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109217"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGD55_04740"
     CDS             complement(<1..704)
                     /locus_tag="VGD55_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869681.1"
                     /translation="MRAPALLRTNRDFRLLWIGESVSELGSTVTLVALPFVALIALHA
                     TAFEVAALAAAETIFWPFIALPIGVWVDRVSRRRVLLIADAGRALALVSIPIAAAMHD
                     LTLAQLYVVGVVAGVLTIFFAVAYPSYLPSVVTTDALVEGNSLLTGAEQVAHISGPAL
                     GGALVQALGAANALFADVASFIVSVVAVFAIRADEPPPVTERRKMRHEVVEGVRFLIE
                     HKVLRAFIVSAAPSNL"
     gene            871..1065
                     /locus_tag="VGD55_04745"
     CDS             871..1065
                     /locus_tag="VGD55_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869682.1"
                     /translation="MSIPVQNAGLLNRGDEPQTNGRRRLGELLVSHGLLTDEQLRQAL
                     AEQERMEGGRRRRLGRVVVD"
     gene            1111..>1927
                     /locus_tag="VGD55_04750"
     CDS             1111..>1927
                     /locus_tag="VGD55_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0869683.1"
                     /translation="MHLELVDLAELSLDLSVARTLPRRVAERHGMLVIGRDDHAIRVA
                     TADPTNVVALDDVRLYTGATTVTVVVSTDTQIQTELRRVWAILDSDAELSNVSDEPEA
                     VDEHDLESAADQGPTVRLATSIVTEAVRSRASDIHVEPQIDGLRIRYRIDGLLRDIMK
                     VPRNSSAALVSRLKIVSGLDIAERRLPQDGRTRLTVDGNGVDARVSTLPSVHGEKVVI
                     RLLASGDHIPPVTQLGLDEQQLEALLAGTANPQGLVLITGPTGSGKTHTLYSVL"
BASE COUNT          375 a          656 c          613 g          283 t
ORIGIN      
        1 aagagattcg acggggcagc cgagacgatg aacgcccgca acaccttgtg ttcgatgagg
       61 aaccgaacac cttcgacgac ctcgtgtcgc atctttcggc gctccgtcac cgggggcggc
      121 tcgtcggctc ggatcgcgaa cacggccacc accgacacga tgaaactagc cacatcggcg
      181 aacagggcgt tggccgcgcc cagtgcctga accagcgcgc cgccgagcgc gggccccgag
      241 atgtgcgcga cctgctcggc cccggtcagt agcgagttgc cttcaacgag tgcgtctgtc
      301 gtgaccacgc tcggcaggta cgacggatac gcaacagcga agaagatcgt gaggacacca
      361 gcgactacgc cgacgacgta gagctgcgca agcgtcaggt cgtgcatcgc ggcggcaatc
      421 ggaatggaga ccagcgcgag cgcgcgaccc gcatcggcga tgagcagcac ccgccgccgc
      481 gaaacccggt cgacccacac gccgatcggc agtgcgatga agggccagaa aatggtctca
      541 gcagccgcta acgccgcaac ctcgaacgct gtggcatgca aggcgatcag tgcgacgaac
      601 ggcagcgcaa cgagcgtgac ggtgctaccg agttcgctga ccgactcccc gatccataac
      661 agccggaagt cacgattcgt gcggagcaga gccggcgcac gcatgcgccc aacctttcag
      721 ttgacccgcc gttcgggtca acgcgttatg cgatcggcgt agcgccaaga gcaacgccag
      781 cccaccgttc ggaggatcta cgggaggcag ttagccgctg tagccccgtt ctgcgcgccc
      841 gacaatgtag gaaacgtgag gagttcggac gtgtcgatac ctgtccaaaa cgctggcttg
      901 ctcaatcgcg gcgacgagcc gcaaaccaac gggcgccggc gccttggtga gttgctggtc
      961 tcgcatggcc tgttgaccga cgagcagcta cgtcaggcgc tcgccgagca ggagcgcatg
     1021 gaaggcggcc gccggcgcag gctcggtcgg gtggtcgtcg attaggggta tcccacggag
     1081 ggccagctcg cgtacgcgct tgccgacctc atgcatttgg aattggtcga cctcgccgaa
     1141 ctcagccttg acctgagcgt cgctcgcacg ctgccgcgtc gcgtcgccga gcgccacggc
     1201 atgttggtga tcggtcgcga cgaccacgcg atcagggtcg caactgcgga tccgacgaac
     1261 gtggtcgccc tcgacgacgt ccggctctac accggagcca ccacggtcac agtcgtcgtc
     1321 agcaccgaca cgcagatcca gaccgagctc cgccgggtgt gggcgatcct cgactcagac
     1381 gccgaactgt cgaacgtctc cgacgagccc gaagccgtcg acgagcacga cctcgagagt
     1441 gcggcagatc agggcccgac cgtccgactc gccacgagca tcgtgacgga ggccgtgcgc
     1501 agccgcgcca gcgacattca cgtagagccg cagatcgacg gtctgcggat caggtaccgc
     1561 atcgacggcc tgcttcgcga catcatgaaa gtgccgcgca actcctccgc cgcacttgtc
     1621 agccgcctca agatcgtctc cggtctcgac atcgcagagc gacgcctgcc gcaagacggc
     1681 cgcacccgac tcactgttga cggcaacggc gtcgacgcgc gcgtcagcac gctgccttcg
     1741 gtgcacggcg agaaggtcgt catccggctg cttgccagcg gcgaccacat tccgccggtg
     1801 acccaacttg gcctcgacga gcagcaactc gaggcgttgc tcgccggcac cgcgaatccg
     1861 caagggctgg tgctcatcac cggcccgacg gggtctggca agacgcacac gctctactcg
     1921 gtgctct
//
LOCUS       DASWVF010000268         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_185644, whole genome shotgun sequence.
ACCESSION   DASWVF010000268 DASWVF010000000
VERSION     DASWVF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185644"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VGD55_04755"
     CDS             complement(<1..609)
                     /locus_tag="VGD55_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869684.1"
                     /translation="MNTAPTLARPVFAHLHRLTDKRGLFEHALHGDPRPEHGYCLDDV
                     SRALVVICREPQPSAALQALGRHYLDFTLSAITADGSCHNRMSTSGTWSDDASLDDCW
                     GRALWGLGVAAAFAPTQAMQARALLGFRTAARARSPHPRALAFAALGAGELVLARGDE
                     PAAEELLRDAVATIGPNGVDPAWPWPQPRLTYGNGSIVEALLL"
     gene            complement(606..1730)
                     /locus_tag="VGD55_04760"
     CDS             complement(606..1730)
                     /locus_tag="VGD55_04760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869685.1"
                     /translation="MTTRFGFVSTYPPTICGLATFTSSLYRELVTSAGNEGRVVRLVE
                     TPQPAAGDEVVGELVAGDPRSAYRAAALLNDGDIAIVQHEYGVYGGPDGDEILVLLRA
                     LTVPSVVVLHTVLSAPTAHQKSVLEEVAAAADAVVTMTITAHNRLATGYAVDMRKVSV
                     IPHGAPLRSRNELSVRTDRPTVLTWGLIGPGKGIEWGVEAMAALGDLYPTPRYVVAGQ
                     THPKVLLREGEAYRDRLKKQVASLSLGDSVSFDSRYRDNVALGELVDSADVVLLPYDS
                     TDQVTSGVLIEAVAALKPVVATRFPHAVELLGDGAGLLVPQKNPAAIAAALRTLFTDH
                     DLAAGMSKAAGATAAELLWSNVAGRYLELADHLISTRVAA"
     gene            2105..3145
                     /locus_tag="VGD55_04765"
     CDS             2105..3145
                     /locus_tag="VGD55_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869686.1"
                     /translation="MAHKINRNRARHITRRIIVMATTGTATAAFGVFGLTPAAHAATH
                     DSAVFVQTNDADGNTVIAYDRANDGSLHVAGHYATGGLGGSEAGAVVDPLASQGSLTY
                     DANHQLLLVVNAGSDTLTVFAVDGSHLHRVQILGSKGHLPTSIGVSGNLAYVLDAGND
                     GAITGFKISGGRLHTIDNSTRSLSLGNPADPNFLSAPSQVAISPDLRDVVVGTKTHGV
                     LDVFTLNHSGVPSTSPAVTTSAGAVPFAVGFDPAGRLLVAEASGGASSYSVQPDGSLT
                     TISSHIANGQAATCWGASAKGFIYVANAGSNSITAYVENSHGELSLLNGSEVAAVTDG
                     GPVDMATSSDGR"
     gene            complement(3371..>3916)
                     /locus_tag="VGD55_04770"
     CDS             complement(3371..>3916)
                     /locus_tag="VGD55_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869687.1"
                     /translation="VLVPEVPFELDGEEGFLRHLRGRVETQGHAVVVVAEGAGQHLLP
                     RDGSRDASGNARLADVGQLMRDRIVECFADAGSELSLRYVDPGYAIRSVPANAYDAVF
                     CIRLAQAAVHAAMAGRTAMMVGRWHGRFVHLPIALATASRNQVDPDGDLWMSVLEATR
                     QPPRFGRSAVLLPQDFAAVGT"
BASE COUNT          730 a         1264 c         1254 g          668 t
ORIGIN      
        1 aagaagaagg gcttcgacga tcgagccgtt cccgtacgtc agtcgcggct gcggccaggg
       61 ccacgcagga tcgacaccgt tcggaccgat cgttgccacc gcgtcgcgaa gcaactcttc
      121 agcagcaggc tcgtcgccgc gcgcaagcac caactcgccc gcgcccagtg ccgcgaacgc
      181 cagagcacgc gggtgcggtg aacgcgcccg cgctgcggtt cgaaatccga gtaacgccct
      241 cgcttgcata gcctgtgttg gtgcgaaagc ggccgcaacg ccgaggcccc aaagtgcgcg
      301 accccagcag tcgtcgaggc tcgcgtcatc gctccaggtg cctgacgtac tcatcctgtt
      361 gtggcacgag ccgtcggcag tgatcgccga caacgtgaag tcgaggtagt gccggccgag
      421 tgcctgcaac gcggcgctgg gctgtggttc gcgacagatg accacgagcg cgcgcgaaac
      481 gtcgtcgagg cagtagccgt gctcgggacg cggatctccg tgcagcgcgt gctcgaacag
      541 gccccgcttg tcggtgagcc ggtgcaagtg agcaaacacc ggcctcgcca gtgtcggggc
      601 ggtgttcatg cagcaactcg agtgctgatg aggtggtcgg ccagctcgag atagcggccc
      661 gcgacattcg accagagcag ttcagccgcg gtagcaccag cggccttgct catgccggcc
      721 gccagatcgt ggtcggtgaa cagcgtgcgc aacgcggccg cgatggctgc cggattcttc
      781 tgcggaacga gcaacccggc accgtcaccg agcagttcga cggcgtgggg gaatcgggtc
      841 gcgacgaccg gcttcagtgc agcgactgct tcgatcagca cgcccgaagt cacctgatct
      901 gttgagtcat acggcaacaa cacgacgtcc gccgaatcga ccaactcacc aagcgcaacg
      961 ttgtcgcgat agcggctgtc gaagctgacg ctgtcaccga gggagagcga cgcgacctgc
     1021 ttcttgagcc tgtcgcggta agcctcgcct tcgcgcagca gcaccttcgg atgcgtctgg
     1081 ccggcgacga cgtagcgagg cgtcgggtag aggtcgccga gtgctgccat cgcctcgacg
     1141 ccccactcga tgcccttgcc gggcccgatc aatccccacg tcagcacggt tggccggtcg
     1201 gtgcgcacgg acagttcgtt cctgctgcgc aggggtgcgc cgtgcgggat cacgctgacc
     1261 ttgcgcatgt cgacggcgta accggtggcg agccggttgt gcgctgtgat cgtcatcgtg
     1321 acgacggcgt cagctgcggc ggcaacttct tcgagcactg acttctggtg tgcggtcggc
     1381 gcactcagca cggtgtgcag caccacgacc gacggcacgg tgagcgcgcg caggagcacc
     1441 aagatctcgt cgccatcggg gccgccgtac acgccgtact cgtgttggac gatcgcgatg
     1501 tcaccgtcgt tgagaagggc ggcggcccgg taggcactgc gcgggtcgcc cgcgacgagc
     1561 tcgccaacaa cctcgtcgcc agcagcgggc tgcggcgtct cgacaagacg gaccacgcgg
     1621 ccctcgttac cggccgacgt caccagctcg cgatagaggg acgacgtgaa agtggccagc
     1681 ccacagatcg tgggcgggta ggtgcttacg aaaccaaagc gagtcgtcac gactaacgcc
     1741 ttccggttga cggcgccgcc agcggtgcgc gaggcctcac gggaacagat tggagatgcc
     1801 gcagctgagc cgcgacgacg ccctcaccca gactgggcga acagggctgc catgcgcccc
     1861 gatataggag cgatcgtttg gcagtctacc ttgcttgcgc catatcgaca gattcgttag
     1921 gccaggcggg tgtgtgacaa cgatcccaat tacttcttac agacttgtga cgtttggttc
     1981 ttacggaacg ctgacaactc tcgttttcga gcccttcagg cgagacggat cttcaatccg
     2041 ctgttagaaa acgctgtggg gccggggaat cagcggctgt cgaaccagcg attggatatg
     2101 tgagatggcg cacaagatca acaggaatcg tgcacgacac atcaccaggc ggatcatcgt
     2161 gatggcgaca acggggacgg cgaccgccgc cttcggtgta tttggactta cgccggcggc
     2221 gcacgcggct actcatgact cggccgtttt cgttcagacc aacgacgccg acggcaacac
     2281 cgtgatcgcc tacgaccgcg ccaacgatgg cagcctgcac gttgcagggc actatgcaac
     2341 cggcggtctc ggtggttctg aagcgggcgc ggttgtcgat ccgcttgcat cgcaaggatc
     2401 gttgacctac gacgcgaacc accagttgct cttggtggtc aacgctggca gcgacacgct
     2461 cacggtcttc gctgtcgatg ggtcacacct gcatcgagta cagatcctgg gcagcaaggg
     2521 acacctgcca acctcgatcg gtgtcagcgg aaaccttgcc tacgtcctcg acgcaggcaa
     2581 cgacggggcg atcaccggct tcaagatctc gggtggccgg cttcacacga tcgacaactc
     2641 cacgcgttcg ctcagtcttg gcaacccggc agatccgaac ttcctgtcgg ctccctcgca
     2701 ggtcgctatc tcgcccgacc ttcgcgacgt cgtcgtcggc accaagaccc acggcgtgct
     2761 cgatgtcttc acgctgaacc acagcggcgt tccctcgacg tcgcctgccg ttacgacatc
     2821 ggccggtgcc gtgccgttcg cggtcgggtt cgatcccgcg ggtcggttgc tcgtcgccga
     2881 agccagtggc ggtgcatcgt cgtattcggt acagcctgac ggttcgctca ccacgatctc
     2941 gtcgcacatc gccaatgggc aggccgccac ctgctgggga gccagcgcga agggcttcat
     3001 ctacgtggcg aacgccggct caaactcgat cactgcgtac gtcgagaact ctcacggtga
     3061 gctcagcttg ttaaacggct ccgaggtggc cgcggtcact gatggcgggc ccgttgacat
     3121 ggcgacgagc agcgatggcc gctagctgta tcagcaagcg accggcgcag gcgtcatcga
     3181 tgagttcaag gtgggcaacg acggatcgct gacaaagatc ggcaccgtga ccgggttcac
     3241 ccccgacaac ggcaccggtc tcgaaggaat cgctgcaacg tagacccgct caccttcggt
     3301 cgagtgcagg aattccgggg tgtccggtcg cggacgtgcc tcggaattcc ttcactcggc
     3361 gaagtagctt tcacgttccg acggcagcga agtcctgagg cagtagcact gcggatcgtc
     3421 cgaagcgcgg tggttggcgg gtcgcttcga gtacggacat ccacaaatcg ccgtccggat
     3481 caacttgatt gcgactcgct gtcgccaacg cgatgggaag gtgcacgaaa cggccgtgcc
     3541 agcggccgac catcatcgcg gtgcggccgg ccatcgcagc gtgcacggcg gcctgagcga
     3601 gacggatgca gaagaccgcg tcataggcgt tcgcaggcac gctgcgaatc gcataaccgg
     3661 gatcgacata gcgcaggctc aactcagatc ctgcatccgc aaagcattcg acaatgcgat
     3721 cgcgcatcag ctgcccgaca tcggcaagac gtgcgttgcc cgacgcatcg cgcgaaccgt
     3781 cgcgtggcag caggtgctga cccgcacctt cagcgaccac gaccaccgca tgcccttgag
     3841 tttcgacgcg gccgcgcagg tgacgcagga atccttcctc gccgtcgagt tcgaacggca
     3901 cctcgggcac gagcac
//
LOCUS       DASWVF010000269         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_236643, whole genome shotgun sequence.
ACCESSION   DASWVF010000269 DASWVF010000000
VERSION     DASWVF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236643"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VGD55_04775"
     CDS             complement(<1..212)
                     /locus_tag="VGD55_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEY0869688.1"
                     /translation="MTALAGSAPTATDLAVVERQLGRTPRAVRGVAHRCECGLADVIE
                     TQPRLPDGSPFPTFFYLTCPRAASAVG"
     gene            complement(209..793)
                     /locus_tag="VGD55_04780"
     CDS             complement(209..793)
                     /locus_tag="VGD55_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0869689.1"
                     /translation="MLRRKPAFTSRAAVLAVVVAALALALAAPLRELLSQRSQISALN
                     SSIATSQQQIAQLQKSQKQLANPSYIESQARDRLHYVFPGQVPYVTLTPTPAPEASAA
                     AALASQPWYGQLWGSVKSASGVTPKPLPSPTPSLAPALVPRVSSPALAPASAPVSDSA
                     VAPPPATLPTTLPSTLASLAASEAAAASAAGNGL"
     gene            complement(796..2076)
                     /gene="eno"
                     /locus_tag="VGD55_04785"
     CDS             complement(796..2076)
                     /gene="eno"
                     /locus_tag="VGD55_04785"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0869690.1"
                     /translation="MAAIEALGAREILDSRGNPTIEVEALLDDGTIARAAVPSGASTG
                     AFEAVELRDGGQRYGGKGVEKAVQAVIDDIAPEVVGFEASEQRLLDAKLIELDGTPNK
                     AKLGANAILGVSLAVARAAAESAELPLFRYLGGPNAHLLPVPMMNIVNGGSHADSNVD
                     IQEFMIAPIGAGTFAEAVRWAAETYHALKAVLKARGLSTGLGDEGGFAPNLASNRDAL
                     DLIIEGITKAGYTPGRDIALALDVAATEFHKDGVYMFEGGGKSSSDLIAYYSDLVANY
                     PLVSIEDPLDEEDWDGWKSITDELGAKVQIVGDDLFVTNPVRIARGIAAGTANALLVK
                     LNQIGTLTETLDAVELAHRAGYRTMVSHRSGETEDTTIADVVVATNSGQIKTGAPARA
                     ERVAKYNQLMRIEEELDDAARYAGRSAFPRFVEG"
     gene            complement(2201..>3155)
                     /locus_tag="VGD55_04790"
     CDS             complement(2201..>3155)
                     /locus_tag="VGD55_04790"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEY0869691.1"
                     /translation="LGAALSCATALLAVLVIAVVPGASGSGAPQSVPSSTVAGFDQSI
                     SGIVENAVSTRIVPSNLTPALETVSGDQPAVGGCHLSFSATKSGPCVFGDRGSSTTVV
                     LFGDSHAEQWFPAMQKIALAKDWKLVVWTKSDCPAVDVSMYSHILKRTYTECNTWRTN
                     TLKQIVALHPKLVVMSSAITLSNVANSGGAKFETAWLAGTTSIVKTVESSGAAVVWIG
                     DTPQPKGNAPDCVASKLKDVRPCMNTALAAELDPARRTQTMALAAAAGATVVDPTPWT
                     CPGTTCPVVVGNLLVYRDDSHLSSAYVDWLGPLLQAKLPLS"
BASE COUNT          527 a         1059 c         1048 g          521 t
ORIGIN      
        1 ccgaccgccg aggcggcccg agggcaggtc aggtagaaga acgtcggaaa cggcgagccg
       61 tcaggcagtc gcggttgcgt ctcgatcacg tcggcaagac cgcactcgca gcggtgggcg
      121 acgccccgaa cagcccgcgg ggtgcggccg agttgccgct cgacgacggc gagatccgtg
      181 gcggttggag ccgaacccgc caatgcggtc acagtccatt acccgcagcg ctggcggcgg
      241 cggcttcgct cgccgccaat gacgccagcg tcgaaggcaa cgtcgtcggc aacgtcgcag
      301 gcggcggcgc gaccgctgag tccgacacgg gagccgacgc gggggccagc gcgggtgagg
      361 acacacgcgg aacaagtgca ggtgcgagcg acggcgtcgg cgacggcagt ggcttgggag
      421 taactccgct ggcactcttc accgaacccc acaactggcc gtaccacggc tgcgaggcaa
      481 gcgccgcggc cgcgctcgcc tcgggcgcgg gtgtcggcgt cagtgtcacg tacggcacct
      541 gaccaggaaa cacgtagtgc aaacggtcgc gcgcctgcga ctcgatgtac gacggattcg
      601 ccaactgctt ctgcgacttt tgcaactgcg caatctgttg ctgcgacgtt gcgatgctgc
      661 tgttcaatgc agagatctgc gagcgttgac tcagtagttc gcgcaacggc gcggccaacg
      721 ccagagccag cgccgccacg acaacggcga gtaccgccgc ccgacttgtg aaggcaggct
      781 tgcggcggag caaggtcagc cctccacgaa ccgcgggaac gcggaccggc ctgcgtaacg
      841 cgccgcgtcg tcgagttctt cttcgatgcg catgagctgg ttgtacttcg caacccgctc
      901 ggcgcgcgcc ggcgcgccgg tcttgatctg cccggaattt gtcgccacca cgacgtcggc
      961 gatcgtggtg tcttcggtct cacccgatcg gtgcgacacc atcgtgcggt agcccgcgcg
     1021 atgcgccagc tcgacggcgt ccaacgtctc tgtcaatgtg ccgatctggt tgagcttgac
     1081 cagcaacgcg ttggcggtgc ctgcggcgat gcccctggcg atccgcaccg gattggtcac
     1141 gaacaagtcg tcgccgacga tctgcacttt cgcgccgagc tcgtcggtga tcgacttcca
     1201 gccgtcccag tcctcttcgt cgagcgggtc ttcgatggac accaacgggt agttcgcgac
     1261 gaggtcgctg tagtacgcaa tcaagtcgga cgacgatttg cccccgccct cgaacatgta
     1321 cacgccgtcc ttgtggaact cggtcgcagc gacgtcaagc gcgagtgcga tatcgcgccc
     1381 aggcgtgtaa ccggccttgg tgatcccctc gataatcagg tcgagcgcat cgcggttgct
     1441 cgcaaggttc ggcgcgaagc cgccctcgtc tccgagccca gtcgacaggc cgcgcgcctt
     1501 cagtactgcc ttcagcgcgt gataggtctc ggccgcccag cgcaccgcct cggcgaacgt
     1561 cccggcgccg atcggagcga tcatgaactc ctggatgtcg acgttggagt cggcgtgcga
     1621 acccccgttg acgatgttca tcatcggcac cggcagcagg tgagcgttcg gaccgccgag
     1681 gtaacggaac agcggcaact cagcggactc ggcggcggcc ctggccactg ccagggagac
     1741 cccgagaatc gcgttggcac cgagcttggc cttgttggga gtgccgtcga gttcgatcag
     1801 cttcgcgtcg agcagccgct gctcgctggc ctcgaacccc acgacctcgg gcgcgatgtc
     1861 gtcgatgaca gcctgcacag ccttctcgac gcccttgccg ccgtagcgct ggccgccatc
     1921 acgcagttca acggcctcga aggcgccggt cgaggccccg ctcggaacag ccgcgcgagc
     1981 gatcgtgccg tcatcgagga gcgcctcgac ttcaatggtc gggttgcccc gcgaatcgag
     2041 gatctcgcga gccccgaggg cttcgatggc ggccacggtg gcactcctga cgcttttgag
     2101 cacggggctg gatttgagcg agcgtcaccg agtttatccg cccgaggcgg ccgccctgcg
     2161 cacagcccgg ctgcccggcg cgcccctgtt cttgcggcga tcagctcagc ggcagcttgg
     2221 cctgcaggag cggcccgagc cagtcgacgt aggcgctgct gaggtgactg tcgtcgcggt
     2281 agaccagcaa gttgcccacg accacagggc acgtcgtgcc cgggcaggtc cacggcgtcg
     2341 ggtcgaccac ggtggcaccc gcggcggccg caagtgccat ggtctgcgtc cgcctggccg
     2401 ggtcgagctc agccgcgagc gccgtgttca tgcacggccg gacgtccttc aacttcgagg
     2461 caacgcaatc gggcgcattg cccttgggct gcggggtgtc accgatccac accacggcgg
     2521 ctccactgct ctcgaccgtc ttcacgatcg aggtcgtgcc tgcaagccac gccgtctcga
     2581 acttggcccc acccgagttg gcgacgttgc tgagcgtgat cgccgatgac atcaccacca
     2641 gcttcggatg cagcgcgacg atctgcttca gggtgttcgt gcgccacgtg ttgcactcgg
     2701 tatacgtgcg cttcaagatg tggctgtaca tcgagacgtc gactgccggg cagtcagact
     2761 tcgtccacac gacgagcttc cagtccttgg ccagggcgat cttttgcatc gccgggaacc
     2821 attgctcggc gtgagagtcg ccgaacagca cgacggtggt cgacgaaccc ctgtcaccaa
     2881 agacgcacgg gcctgacttc gtcgcgctga agctgaggtg gcagccgccg acggccggct
     2941 ggtcgccact aacggtctca agggcgggcg tgagatttga cggcacgatg cgggtgctca
     3001 cggcgttttc gacgattccg gagatgcttt ggtcgaagcc ggcaacggtc gaagacggca
     3061 ccgactgcgg cgccccgctg cccgaagcgc cgggaactac agcgatcacc agcacggcaa
     3121 gaagcgcagt cgcgcacgac aaggcggcgc cgagc
//
LOCUS       DASWVF010000270         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_465978, whole genome shotgun sequence.
ACCESSION   DASWVF010000270 DASWVF010000000
VERSION     DASWVF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465978"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGD55_04795"
     CDS             complement(<1..551)
                     /locus_tag="VGD55_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0869692.1"
                     /translation="MSDRVPREVTGSLSELTDIRSARVRAAKRLQKRSFREHDRAFLA
                     EGVQAVREALIEATRGRTGVRALFVTADARVRHRELIEQAVALGVPVHQASGHVMTEL
                     SDTVTPQGLVAVCDVLDVSLDEALSAPRGPASPGLVVLLAAVRDPGNAGTVLRTADAA
                     GASAVVFSDSSVDPYNGKCVRASS"
     gene            complement(576..971)
                     /gene="rplT"
                     /locus_tag="VGD55_04800"
     CDS             complement(576..971)
                     /gene="rplT"
                     /locus_tag="VGD55_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0869693.1"
                     /translation="MARVKRAVNAQKKRRVVLERASGYRGQRSRLYRKAKEQLLHSAT
                     YSYRDRRARKGDFRRLWIQRINAAARLHDMTYNRLIQGLKAAGVEVDRKNLAELAVSD
                     DAAFAVLVEVARKALPAASAGSAGAETAA"
     gene            complement(1044..1238)
                     /gene="rpmI"
                     /locus_tag="VGD55_04805"
     CDS             complement(1044..1238)
                     /gene="rpmI"
                     /locus_tag="VGD55_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0869694.1"
                     /translation="MPKNKTHSGAKKRFWVTGSGKLMRRRAGRNHLLEHKPSTLTRRL
                     NNEVELSAPDSKRAKKLLGL"
     gene            complement(1275..2312)
                     /gene="infC"
                     /locus_tag="VGD55_04810"
     CDS             complement(1275..2312)
                     /gene="infC"
                     /locus_tag="VGD55_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0869695.1"
                     /translation="MPWKPCMRRRAGLLACPGHPGPQHPRPRKQFARTAHVLSAPRGG
                     PISVEPRINDRIRVPEVRLVGPNGEQVGIVRIEDALRLAEEADLDLVEVAPMARPPVA
                     KLMDYGKFKYESAVKAREARKNQAQTVIKEMKLRPKIDVHDYETKKGHVVRFLREGDK
                     VKITIMFRGREQSRPEMGARLLQRLAEDVVELGFVESAPKLDGRNMIMVLGPHKKKAD
                     FKAERNAGRAHDDTPDAPERDLAQTLLAEHAPPALEADEVAPEIAPEPVAPQPAVAQV
                     VAPPAPAVKAAAAPKEPPAPRALATPRAPAAASPKAPTPKAPTPKAPPKPRAPAAKKA
                     AQTADEADATA"
     gene            complement(2497..2847)
                     /locus_tag="VGD55_04815"
     CDS             complement(2497..2847)
                     /locus_tag="VGD55_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869696.1"
                     /translation="MVTRVLAALAVVFVAVAVLLLGPIHATGVRGTALHPRYVPYYVG
                     FFSYSPAGPANAKAALAQAGITAPDVAVHRRIEAAVASAGVAALIGGVLLVRSRRAAE
                     LGVPAAAREDQERR"
BASE COUNT          479 a          994 c         1011 g          551 t
ORIGIN      
        1 gatgacgccc gcacgcactt gccgttgtag ggatcgactg agctgtcgct gaacaccacc
       61 gcagacgcac cggcggcatc ggcggtccgc aacaccgttc cggcattgcc aggatcgcga
      121 acagcggcaa gcaggactac gagcccaggc gatgcgggcc ctcgcggcgc cgacagggcc
      181 tcgtcgagtg acacatcgag cacgtcgcac accgcgacca gaccctgagg cgtcacggtg
      241 tcggacagct cggtcatgac atgcccactg gcttgatgaa cggggacgcc gagggcaacc
      301 gcttgctcga tcagctcacg atgcctgacg cgggcgtcgg ccgtgacaaa cagggcgcga
      361 acaccggtgc ggccgcgcgt cgcctcgatg agggcctcgc gaaccgcctg cacaccttcg
      421 gcgaggaacg cgcgatcgtg ctcgcgaaac gacctctttt gcagacgctt cgcggctcgc
      481 acacgagccg accggatatc ggtcaactcg ctgagcgatc cggtcacctc gcgtggcacc
      541 cggtcactca ccttgttgcc ttggactcag gggcttcagg ccgcggtctc ggcgcccgcg
      601 gaccctgctg atgctgccgg cagtgccttg cgggccacct cgaccagcac cgcgaacgcg
      661 gcgtcgtcgg acacggcaag ctcggcgagg ttcttgcggt cgacttcgac gccggccgcc
      721 ttcaaaccct ggatcagccg gttatacgtc atgtcgtgca acctggctgc ggcgttgatg
      781 cgctggatcc acagccggcg gaagtcgccc ttgcgcgcac ggcggtcgcg gtagctatac
      841 gtcgccgagt gcagcaactg ctctttcgcc ttgcggtaga gccgcgagcg ctggccgcgg
      901 taaccgcttg cccgctcgag aacgacccgg cgcttcttct gggcgttgac tgcccgcttc
      961 acgcgtgcca cgtgggaact ccttgttcag tccggcaccc gctcgcgaga gcggaaccgg
     1021 ttcatcggac gacgaggttc gaactacaga ccgagcagct tcttcgcgcg cttggaatct
     1081 ggggcagaca actcgacctc gttgttgagg cggcgtgtca gcgtggaggg cttgtgctca
     1141 agcaagtggt tgcgcccggc ccggcgccgc atgagcttgc cggaaccggt gacccagaat
     1201 cgcttcttgg caccactgtg ggtcttgttc ttcggcatgg tcgccgcgtc tccttcgtct
     1261 cgcgtccgcc gtacttaggc ggtggcgtca gcctcgtcgg ctgtctgggc tgccttcttc
     1321 gctgccggag ctctgggctt cggcggcgct ttgggcgtgg gcgctttcgg ggtcggtgcc
     1381 ttcggacttg ctgctgctgg agctctcggt gttgcgagag ctcttggagc tggcggttcc
     1441 ttcggtgcgg cggctgcttt caccgctgga gcgggtggtg ctactacctg cgccaccgct
     1501 ggttgcggtg ctaccggttc tggtgcgatt tctggtgcga cttcgtcagc ctcgagcgcc
     1561 ggcggtgcgt gttcggctag cagtgtctgg gcgagatcgc gctcaggtgc gtcaggggtg
     1621 tcatcgtgcg cgcggccggc gttgcgttcg gccttgaaat cggccttctt cttgtgcggc
     1681 ccgagcacca tgatcatgtt gcgtccgtcg agcttcggcg ccgactcgac gaacccgagt
     1741 tcgacgacgt cttcggccaa ccgctgcagg agccgcgcgc ccatctccgg tcgcgactgc
     1801 tcgcgccccc ggaacatgat ggtgatcttg accttgtcgc cttctctgag aaagcgcacg
     1861 acgtgaccct tcttggtctc gtagtcgtgc acatcgatct tgggtcgaag cttcatctct
     1921 ttgatgaccg tttgggcctg gttcttccgc gcctcgcggg ctttcaccgc ggactcgtac
     1981 ttgaactttc cgtagtccat gagcttggcc acggggggcc ttgccatggg cgcgacctcg
     2041 actaggtcga gatctgcctc ttcggccaaa cgaagtgcgt cttcaatgcg gacgatgccg
     2101 acctgctcgc cgttgggacc gacgagccga acctcaggaa cacggatccg gtcgttgatg
     2161 cggggctcga cgctgatggg acctcctctt ggagcggata ggacatgtgc tgttcgagcg
     2221 aactgcttgc ggggcctcgg gtgctgtgga cctggatggc ccggacacgc aagaagcccc
     2281 gcacgacgtc gcatgcaggg cttccacggc atggccaggc gccgccaaca aaggcggcac
     2341 catcaccggc ggcgtaaacg cctcggcggc cgaccgaacc tatcggcgcg agcgccgatc
     2401 aggtgggaga agctccactt gcggatgcaa cgcataagcg tcacatcggt cgctcacgga
     2461 gcctaccagg cgaccaggtg tccggccgaa cgcgcgctat cggcgctctt ggtcctcccg
     2521 cgcggcggcg ggaaccccca actcggcagc gcgtcgtgac cgcacgagca gaaccccgcc
     2581 gatcagtgca gcgacgccgg ctgaggcgac tgcagcttcg atacgacggt gcaccgcgac
     2641 gtccggcgcc gtgatgccgg cctgcgccag cgccgctttc gcgttcgctg gaccggccgg
     2701 ggagtacgaa aagaagccca cgtagtacgg cacatagcgc ggatgcaggg ccgtgccacg
     2761 gacgccggtc gcgtggatcg gcccgagcag caacaccgcg accgcgacaa acacgaccgc
     2821 cagcgcggcc agtacccgag tgaccacgcg gtgatcatgc acgaagtagg tagcccgatg
     2881 ctgttccgct gccaaattcg tgcatgatca tcggaactga gggcggaaac tgagggcgga
     2941 aactgagggc gggacgccga ggggctgggc tcaagcgaga tcgcggctgt cccgacaatc
     3001 cggtatggcg accgaacgag cacgcggatg acggc
//
LOCUS       DASWVF010000271         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_262291, whole genome shotgun sequence.
ACCESSION   DASWVF010000271 DASWVF010000000
VERSION     DASWVF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262291"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /locus_tag="VGD55_04820"
     CDS             <1..>1608
                     /locus_tag="VGD55_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0869697.1"
                     /translation="WATRAGVTISISDVVTPPRKQEILEEYEAKADKVQKQFERGLIT
                     DDERRQELIEIWTQATADVAREMEGNFPKDNPVWMMVNSGARGNMMQVRQIAGMRGLV
                     ANPKGEIIPRPIKANFREGLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVSQ
                     DVIIREEDCGTERGIMMRIAAPGRDGVLVRPATVETSVFARTIAEDIIHDGKLVGEAR
                     GDLGDLSIGALVEHGVQELKVRSVLTCESKVGTCAACYGRSLATGKTVDVGEAVGIIA
                     AQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRVVELFEARTPKGVAPISEVTGRVKIE
                     DTDKARKIVVVPDDGAEEIAYPVSKRSRLRVAEGDHVEVGEQLIEGTVNPHEVLRILG
                     PRAVQVHLVQEVQEVYRSQGVPIHDKHIEIIVRQMLKRVNVLESGDTDFLPGELVERP
                     RFEEGNRRVVAEGGTPASARPVLMGITKASLATESWLSAASFQETTRVLTDAAIHAKS
                     DPLLGLKENVIIGKLIPAGTGLPRYRN"
BASE COUNT          291 a          531 c          540 g          246 t
ORIGIN      
        1 actgggccac ccgcgccggc gtcacgatct cgatctccga cgtcgtcacg ccgccgcgca
       61 agcaggagat cctcgaggag tacgaggcca aggccgacaa ggtgcagaag cagttcgagc
      121 gtgggctcat caccgacgac gaacgtcgtc aggagctcat cgagatctgg acccaggcca
      181 ccgccgacgt tgcccgtgag atggaaggca acttccccaa ggacaacccg gtctggatga
      241 tggtcaactc cggcgcacgc ggcaacatga tgcaggtgcg gcagatcgcc ggcatgcgtg
      301 gtcttgtcgc caaccccaag ggtgagatca tcccgcggcc gatcaaggcg aacttccgtg
      361 aaggcctttc cgtgctggag tacttcatct ccacccacgg cgcccgtaag ggtctggccg
      421 acacggcgct tcgcaccgcc gactccgggt acctcacccg tcgtctcgtc gacgtgtctc
      481 aggacgtcat catccgcgag gaagattgcg gcaccgagcg cggcatcatg atgcggatcg
      541 cggcgcccgg tcgtgacggt gtgctggtgc ggccggcgac ggtcgagacc agcgtgttcg
      601 cccgcacgat cgccgaagac atcattcatg acggcaagtt ggtgggtgag gctcgcgggg
      661 acctcggtga cctgagcatc ggcgcgctcg tcgagcacgg cgtgcaggag ctcaaggttc
      721 ggtcggtgct cacgtgtgag tcgaaggtcg gcacgtgtgc ggcctgctac ggccgctcat
      781 tggccaccgg caagaccgtc gacgtcggcg aagcggtcgg catcatcgcg gcccagtcga
      841 tcggcgagcc cggcacccag ctgacgatgc gtaccttcca caccggcggt gtggcgggtg
      901 aagacatcac gcacggtctg ccgcgcgtgg tcgagttgtt cgaggcacgc acccccaagg
      961 gtgtcgcccc gatcagcgag gtcaccggcc gggtcaagat cgaagacacc gacaaggcac
     1021 gcaagatcgt cgtcgtccct gacgacggtg ccgaggagat tgcctacccg gtgtcgaagc
     1081 ggtcgcggct gcgcgtcgcc gaaggcgacc acgtcgaggt cggcgagcag ctgatcgagg
     1141 gcaccgtcaa cccgcacgag gtgctgcgga tcctcggccc acgcgccgtg caggtccacc
     1201 tggtccaaga ggtccaggag gtctaccgga gccagggtgt gccgatccac gacaagcaca
     1261 tcgagatcat cgtgcggcag atgctgaagc gggtgaacgt gctggagtcg ggcgacaccg
     1321 acttcctgcc gggcgaactc gtcgagcgtc ctcggttcga ggaaggcaac cggcgcgtcg
     1381 tcgcggaggg cggcacaccg gcgtccgctc gaccggttct catgggcatc accaaggcct
     1441 cgctcgcgac cgagtcgtgg ctgtcggccg cctcgttcca agagaccacg cgggtactga
     1501 cggacgccgc gatccacgcg aagtccgacc cgctgctcgg tctcaaggag aacgtcatca
     1561 tcggcaagct catcccggcc ggtacgggcc tgccccgcta ccgcaaca
//
LOCUS       DASWVF010000272         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_185729, whole genome shotgun sequence.
ACCESSION   DASWVF010000272 DASWVF010000000
VERSION     DASWVF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185729"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..850)
                     /locus_tag="VGD55_04825"
     CDS             complement(74..850)
                     /locus_tag="VGD55_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0869698.1"
                     /translation="MRFMLHAEWTKLRTSAGTFWLLLAAMSLTIGLGAVVDAGVSCSS
                     AACGQDPAKLSLSGVYLGQVFVAISAVTIISGEYGTGMIRTTLTAMPNRVGVLVAKAV
                     VAVGWAFAAGLVGVVGSAVLGQILLPDKGFTPAHGYASVALSHGGTLRAVSGSVLYLV
                     LIALLSLGIATVVRDSAAGIGVVLGLLFAFPIALVTVSNPHWHRHLEQLAPMNAGLGI
                     QTTIDVHSLPLTPWAGLGVLGVWAAGALVLGAFVLQLRDA"
     gene            complement(847..>1808)
                     /locus_tag="VGD55_04830"
     CDS             complement(847..>1808)
                     /locus_tag="VGD55_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0869699.1"
                     /translation="GQNWTQVGVARLTGLPLSVPAGLFVATPDNDVTTAAIVGTSTQG
                     GPSQATATFDDVTVHGQSSGSWQGGQFGANGVGDLPPFTDSGGTFSVTGTGDIAPMAS
                     DGGDDNTISGGLIGAFVGLMAMVVIGALFMTSEYRRGLIRTTLVASPARGRVLAAKAV
                     VLACVVFVLGLAASAFTMWYVDRSRRNNGTYIPPASSVTEIRVLVGTAAVLAVAAVLA
                     LAVGAIVRRSAATVAAVIAVTVLPYILGVSSIGALQWMLRVTPAAGFAVQQTLHRYPQ
                     VDGVYIASAGYYPLSPWEGFAVLLGWTAVALGLAVFLLRKRDA"
BASE COUNT          375 a          608 c          570 g          255 t
ORIGIN      
        1 ccttatcgga agacttcgaa catctcaggt ggttcgacct gccagcccaa tgcctcacgg
       61 tcggctgaca gagttacgcg tcgcgcagct gcaacacaaa tgcgccgagg acaagagcgc
      121 ctgcggccca caccccgagt acaccgagcc cggcccacgg cgtgagcggc aggctgtgca
      181 cgtcgatcgt cgtttgaatt cccagcccgg cgttcatcgg cgcgagctgc tcgaggtggc
      241 ggtgccagtg cgggttgctc accgtgacga gggcaatcgg gaatgcgaac agcaacccca
      301 acaccacgcc gatgcccgcc gccgagtcgc gcactaccgt cgcaatcccc aggcttagaa
      361 gggcaatgag gacgagatag agcaccgagc cagagaccgc acgcagcgtt ccgccgtgcg
      421 acaacgcgac cgacgcgtag ccgtgagcag gtgtgaatcc cttgtcgggc aacaagattt
      481 ggccgagcac cgccgatccg acaacgccaa ccaaaccagc tgcaaatgcc caaccgacgg
      541 cgacaacggc cttcgctacg agcacgccaa cgcggttggg catcgccgtc agcgtggtgc
      601 ggatcatgcc ggtgccgtac tcgccgctga tgatcgtcac cgcagagatg gccacgaaca
      661 cctggcctag gtagacaccg ctgaggctta gctttgcggg gtcttggccg caggctgcgg
      721 agctacagct cacgcccgcg tcgacgaccg cgccgagccc gatggtcagg ctcatcgctg
      781 cgaggagcaa ccagaaagta cctgcggacg tgcgaagctt cgtccactct gcgtgcagca
      841 tgaacctcat gcatctcgct tgcgcaacag gaagacggcc aggccgagag cgaccgctgt
      901 ccaaccgagc agaaccgcga atccctccca cggcgacagc gggtaatagc ccgcagacgc
      961 gatgtagacc ccatcgacct gcgggtagcg gtgcaaagtt tgctggacgg cgaagccggc
     1021 cgccggagtc acccgcagca tccactgcag agctccgatc gacgacacgc cgaggatgta
     1081 cggcaacacc gtgaccgcga tgaccgccgc aaccgtagcc gcactgcggc gcacgatcgc
     1141 gccgacagcc agagcaagga ccgcggcaac ggcgagcacg gcggcggtgc cgactaacac
     1201 tcggatttcc gtgactgagc tggccggcgg gatataggtg ccgttgtttc gccgcgagcg
     1261 gtcgacgtac cacatggtaa aagcagaggc ggctaagcca agaacaaaaa cgacgcaagc
     1321 cagcacgacc gccttcgctg cgagtacgcg gccgcgggca gggctcgcga ccagcgtcgt
     1381 tcggataagg ccgcgacggt actcggacgt cataaacaac gctccgatca cgaccatcgc
     1441 catcagtccg acgaacgcgc caatgaggcc gccgctgatc gtattgtcgt cgccgccgtc
     1501 tgatgccatc ggcgcgatgt cacccgtccc ggtgaccgag aaggtaccgc cggagtcagt
     1561 gaacggtggc agatcgccaa cgccgttggc gccgaactgg ccgccctgcc agctaccgga
     1621 cgactggccg tgcaccgtga cgtcgtcgaa ggtagccgtc gcctgcgaag ggccgccctg
     1681 cgtcgacgtg ccgacgattg ccgctgtggt gacgtcgttg tcgggcgtgg cgacgaacaa
     1741 tcctgctggc accgacaacg gcaatccggt aagccgcgcg acgccaacct gggtccagtt
     1801 ctggccgt
//
LOCUS       DASWVF010000273         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_58162, whole genome shotgun sequence.
ACCESSION   DASWVF010000273 DASWVF010000000
VERSION     DASWVF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58162"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VGD55_04835"
     CDS             <1..1260
                     /locus_tag="VGD55_04835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY0869700.1"
                     /translation="EWKPGRMTALMDLMGDPHLAYPSVHIAGTNGKTSTSRMIDALLR
                     SLDLRTGRFTSPHLESITERIAIDGEPISAALFVQTYDEIAHFLELADSKGDIRLSFF
                     EAVTAMAFAAFADAPVDVAVVEVGLGGRLDATNIVNAPVAVITPVDLDHMAYLGDTIG
                     AIAAEKAGIIKPDQQVIVAAQHPDAISVLLSRAEEVGANALREGFDFGVIRREVAVGG
                     QVLALRGLHGVYDEVFLPLYGGYQASNASCAVAAVEAFAGAGAPLDADLVRAAFSTVQ
                     SPGRLEIVRTSPTILVDATHNPAGARATVAALAEAFAFRRLVGVVAVFADKDVVGLLE
                     VLEPFLAHVVVTQSDSSRAMPADELAAIAIDIFGADRVDVATHLDEAISIAVERADED
                     DDFGGAGVLITGSVAAAGQARRLLKRR"
     gene            1257..1724
                     /locus_tag="VGD55_04840"
     CDS             1257..1724
                     /locus_tag="VGD55_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEY0869701.1"
                     /translation="MTARLGKPPLQSRPPLAPSEYDGDDVALMYRDAIKGKRRLTSSV
                     LSMEFVVFWLAIIVAIVQSHVRVAVAVAVGGALAVGCVAVAAMIRRSWAYKAGGALQV
                     LAILCGFVVPAMFVVGVLFAVLWTVAVRLGDGAFRRAKVFEEYVGAGPNTPDE"
     gene            1789..>2068
                     /locus_tag="VGD55_04845"
     CDS             1789..>2068
                     /locus_tag="VGD55_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0869702.1"
                     /translation="MTDPEPQALIHARGLVKKFGEFVAVDGIDFDVAQGEAFGFLGPN
                     GAGKSSTMRMIGCVSPPTAGTLSILGMDPHRDGAAIRGRLGVVPQQDTL"
BASE COUNT          317 a          652 c          707 g          392 t
ORIGIN      
        1 gaatggaaac ccggtcgcat gaccgcgctc atggatctga tgggcgatcc gcatcttgcg
       61 tatccatcgg tgcacatcgc cggcaccaac ggcaagacat cgacgtcgcg catgatcgat
      121 gcgctgctgc ggtcacttga cctgcgcacc gggcgcttca ccagtccgca cctggagtca
      181 atcactgaac gcatcgcaat cgacggtgaa ccgatcagtg cagccttgtt cgttcagacg
      241 tacgacgaga tcgcccattt cctcgagctg gccgacagca agggtgacat ccggttgtcg
      301 ttcttcgagg cggttaccgc gatggcgttt gcggcattcg cggacgcccc cgtcgatgtg
      361 gccgtcgtcg aggtcgggtt gggcggtcgg ctcgatgcaa cgaacattgt gaacgcgccg
      421 gtggcagtga tcacccccgt tgacctcgat cacatggcct acttgggcga caccatcggc
      481 gccatcgccg ctgaaaaggc cggcatcatc aagcccgacc agcaggtgat cgtcgccgcg
      541 caacaccccg acgcgatctc ggtgttgctc tcgcgcgcag aagaggttgg cgccaacgcg
      601 cttcgcgagg gattcgactt cggcgtcatt aggcgcgagg ttgcggtcgg cggtcaggtg
      661 ctcgcactgc gcggcttgca cggcgtctac gacgaggtgt tccttccgct gtacgggggc
      721 taccaagcat cgaacgcgtc gtgcgcggtc gccgcagtcg aggcgtttgc cggcgccggc
      781 gcgcctctcg acgccgacct cgtgcgggca gccttctcga cggtgcaatc gcctgggcga
      841 ctcgagatcg tgcgcacgtc gccgacgatc ctggtcgacg cgacgcacaa tcccgccggt
      901 gctcgcgcca ctgtcgcagc gctcgccgag gcattcgcgt tccgccgact cgtcggcgtg
      961 gttgccgtct tcgccgataa ggacgtcgtc ggcctgctcg aggtgctcga accgtttctc
     1021 gcccacgtcg tcgtcacgca gagcgactca agtcgcgcga tgccggccga cgagctggcc
     1081 gcgatcgcga tcgacatctt cggcgccgat cgggtcgacg tcgcgacgca tctcgatgag
     1141 gcgatcagca tcgccgtaga gcgagcagat gaggacgacg acttcggcgg ggccggtgtc
     1201 ttgatcaccg gctcggtcgc agcggctggc caagcgcgac ggttgctgaa gcgccgatga
     1261 ccgcgcgtct aggcaaaccg ccgctgcagt cgcgaccgcc gctcgcaccg tcggagtacg
     1321 acggcgacga cgtcgcgctg atgtatcgcg atgccatcaa gggcaagcgc cgactcacgt
     1381 cgagcgtgct gtcgatggag ttcgtcgtct tctggctggc gatcatcgtg gcgatcgtgc
     1441 agtcacatgt gcgagttgcg gtcgcggtcg cggtcggtgg tgcgctggcc gtcggttgtg
     1501 tcgctgtcgc ggcgatgatc aggcggtcgt gggcttacaa ggcggggggc gcgctgcagg
     1561 tgctggcgat cctgtgcggc ttcgtcgtcc cggcaatgtt tgtcgtggga gtccttttcg
     1621 ccgtgctgtg gaccgtcgcg gttcgcttgg gagacggtgc gtttcggcgc gcgaaggtgt
     1681 tcgaggagta cgtcggcgct gggccgaata cgccagacga gtaggccgct ggtcacactg
     1741 ggcgctaact cggttgggac tatcggcggg ccacgataac gtcgagatat gaccgacccg
     1801 gaaccgcagg cgctgatcca tgcccgcggc ctggtcaaga agttcggcga gttcgttgcg
     1861 gtcgacggca tcgacttcga cgtcgcccag ggcgaggcgt tcggattcct cggtcccaac
     1921 ggcgccggca agtcgtcgac gatgcggatg atcggttgcg tttctccgcc caccgctggc
     1981 acgttgtcga tcctgggcat ggatccgcat cgcgatgggg cggcgattcg tgggcgactc
     2041 ggcgtcgttc cgcaacaaga caccctcg
//
LOCUS       DASWVF010000274         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_262425, whole genome shotgun sequence.
ACCESSION   DASWVF010000274 DASWVF010000000
VERSION     DASWVF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262425"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VGD55_04850"
     CDS             complement(<1..412)
                     /locus_tag="VGD55_04850"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869703.1"
                     /translation="MPVADDAANIYNHDGPNMLSPVVQGFPNLVYVPNSNSNTVDVIN
                     PTTGAIVEHFAVPAQPQHVVPSYDLKTLWVNSDVGNALTPIDPATGLPGMPITVADPY
                     NLYFTPDGAYALVMAERLHRIDWRDPHTMALIHTT"
     gene            complement(718..1653)
                     /locus_tag="VGD55_04855"
     CDS             complement(718..1653)
                     /locus_tag="VGD55_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0869704.1"
                     /translation="MERESVRDETAKVGRRTVLKVGFAVAVAGVAGVGCTTGLHPTTP
                     GAASTSSDTAPSPVRSTTDAPATSTPAISTPSASPSASPSASPAESELASASPSSSAA
                     HSSAPVTPGSPALEIVHGPASATGVALTFHGAGDVVLATKLLGELEAAGARATVLAVG
                     QWLDQQPAMAKRILAGGHELGNHTYRHLTMPPLTETVDESEIIRCADVLRRLTGSAGR
                     WFRPSGTVHATPAILAAAGRAGYATSLSYDVDPSDYADPGAAAVTSRVLAAVRSGSIV
                     SLHLGHLGTVQAMPAILDGLRSRGLAAVTMSELMG"
     gene            complement(1923..3068)
                     /locus_tag="VGD55_04860"
     CDS             complement(1923..3068)
                     /locus_tag="VGD55_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869705.1"
                     /translation="MVAAVAGALAVLFVAARDLTHGAHLGAFALASSVFVHPGASADL
                     PVHTGTNGYDGQFYYRLALDPLTHRATAFHITLDNPPYRQQRIGLPATAWFVREVFRA
                     PTSLTLVLVNVLALVAVGWAAAVWARQYGRSTWWGLALAASPALVMALARDLTEPLAT
                     AALVIGLLAWTRRKWWLASMAFTAAVLCRETVLIVLLGMGVWCLVRVLRGGGLIRRRT
                     GVAQVVALCVPAAVEVSWQFHVREVWGGKLPTLSGSNQVGFSLLRPARSFFYNASGIG
                     FSHQGILDGIWLLERFLLATFLVAVLSGLLRSAAPPELRLGWIFAAVLALTVAWNQDV
                     EFVRAANEAIIVGQLLLLARGDRVARAAVTGVFGWSALVAVVYAAAL"
     gene            3304..3792
                     /locus_tag="VGD55_04865"
     CDS             3304..3792
                     /locus_tag="VGD55_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869706.1"
                     /translation="MLNVMHERSTGFEPRRETVAAADPRLTDPFVGMVAFGATLAVAV
                     ALARAGSAVGWGTQLAAMAVTVALVCWWCRVLPSLFVALLGWLMLNGIVVNGAGRLGW
                     DGHQDLIRIGVLLGTGFGVALARRLELQLRSVPTGSPAVETDRHLYVVRPDNELQGDR
                     HA"
     gene            3865..3954
                     /gene="kdpF"
                     /locus_tag="VGD55_04870"
     CDS             3865..3954
                     /gene="kdpF"
                     /locus_tag="VGD55_04870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY0869707.1"
                     /translation="MSAENLIGLILAIGVTIYLVVALLFPERF"
     gene            3955..>5251
                     /gene="kdpA"
                     /locus_tag="VGD55_04875"
     CDS             3955..>5251
                     /gene="kdpA"
                     /locus_tag="VGD55_04875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0869708.1"
                     /translation="MHATAAGILFLVSLVIALALSYRPLGDYMARVFSSKHHWHAERV
                     IYRAIGVNPDADQKWSTYLRSMLAFSLVSVLGLYFMLRAQHHLPFSLGYKGMEQGQSF
                     NTAASFVTNTNWQSYSGESTLGYTAQMVGLAVQNFASAAVGICVAIALVRGFARSRTD
                     QLGNFWVDLIRTVTRILLPISVVFAVILLAGGAIDNFHATHVVNTIAGSHQSITGGPI
                     ASQEAIKQLGTNGGGPYNANSSHPFENPTNWTNWFQIYFLLVIAFSLPRTFGKMVGDK
                     RQGLAIVAVMAILSILSVIGLNVAQGVHHGSVPTSVGAASEGTETRFGVLDSATFASA
                     TTLTSTGAVDSLHDSYTSLGGAITLLDIQLGEVAPGGTGSGLYGILVLAVITVFVAGL
                     MVGRTPEYLGKKIASREMKFSSLYFLTTPAFVLIGTAVAM"
BASE COUNT          927 a         1667 c         1691 g          966 t
ORIGIN      
        1 gtgtcgtatg gatcaacgcc atcgtgtgcg ggtctcgcca gtcgatgcga tgcagccgct
       61 ctgccatgac gagcgcatac gcgccgtccg gtgtgaagta aaggttgtaa ggatcagcga
      121 ccgtgatcgg catgcctggt aacccggttg ccgggtcgat tggagtcagc gcgttgccga
      181 cgtcgctgtt gacccacaag gttttcaggt catacgacgg aacgacatgt tgcggctgag
      241 caggcactgc gaagtgctcg acgatcgcgc cggtcgtcgg gttgatgacg tcaacggtgt
      301 tgctgttgct gttcggtaca tacacgaggt tcgggaaccc ctgcacgacg ggactcagca
      361 tgttcgggcc gtcgtggttg tagatgtttg ccgcgtcgtc ggcaaccggc atgcctggca
      421 gcggatcggc aacacccgat gctaacggcg tcggagtcgc ccccgtcggt tctggcgtgg
      481 ggctcgcgat cgcactcgag ctcgttggag ttgcgcttac gggtggggcg gcagtcgcac
      541 gtggggcagt tgggtcagca gtcggtgcga cagcttgtgg acgtgtggcg atccggggtg
      601 cgaagtcatc acggtcgctt gccgtgcaac ccgttaccac cgcggcgagc aacaagaacg
      661 tcgccagctt ccgcgggaat ccgggcctga tacgtgaatc ttcgaacaca gcgaatctca
      721 acccatcaac tcagacatcg tcacagcggc caaaccccga gagcgtaggc catcgagaat
      781 cgcgggcatc gcctggactg tccccagatg gccgaggtgc agcgacacga tagatcccga
      841 gcggacggcg gccagcactc tcgaggtgac ggctgccgct ccggggtctg cgtagtcgga
      901 cgggtcgacg tcgtacgaca ggctcgtcgc gtaccccgca cgaccggccg ccgcgagaat
      961 cgccggcgtc gcatgcactg tgccagacgg ccgaaaccag cggcccgcag agcccgtcaa
     1021 ccgtcgcagc acgtcagcac agcggatgat ctctgactcg tcgaccgtct ccgtcagtgg
     1081 cggcattgtc aggtgccggt aggtgtggtt gcccagttca tgaccgccag cgaggatccg
     1141 ctttgccatc gcgggctgct ggtcgagcca ctgcccgact gcgagcacgg ttgccctggc
     1201 accagcggcc tcgagttcgc cgagcagttt cgttgcaagc accacgtcac ccgcgccgtg
     1261 gaacgtcagc gcgacaccag ttgccgacgc ggggccgtgc acgatctcga gcgcaggtga
     1321 tcccggcgtc acgggagccg aggagtgtgc agccgacgac gatggcgatg ccgaagcgag
     1381 ttcgctctcg gcgggacttg cactgggact tgcactggga cttgcactgg gtgtgcttat
     1441 ggccggcgta cttgtggcgg gcgcgtcagt tgtcgaccgt actggggacg gtgccgtgtc
     1501 gctgctggtg ctcgcggcac ccggtgtggt cgggtgcaaa ccggtggtgc agccgacgcc
     1561 cgcaacgccc gccaccgcca cggcaaagcc taccttgagc acggtgcgac gcccgacctt
     1621 tgcggtctcg tctcgcacac tctcacgttc catggcgtcg aacctacgcg acgcaagcga
     1681 gaaggtctac gagacgcgaa atccttacag cttaacgatt tcttcaggtc tgtgggtcag
     1741 cgtttcgaaa ggcggcgccc gatctaaccc ccacagattg agcgccgccg acctctcttt
     1801 tgcaccctga tttcgtcacg gaatcgtcag gttgatgcag ctactcttcg cggggtgacg
     1861 cccggccctt ccctcccgtt gggtcgggcg ctaccacctg aattggctag agcctgaatt
     1921 gcctagagcg cggcggcgta caccaccgcg accagcgccg accacccgaa cactccggta
     1981 acggctgctc gggcgacgcg atccccgcgc gccaggagca atagctgacc gacgatgatc
     2041 gcctcgttgg cggctctcac gaactcgacg tcctgattcc aggcaacggt cagtgccagc
     2101 accgccgcga agatccagcc gagtcgcaac tccggcggcg ctgccgatcg cagcaatccg
     2161 ctgagaactg cgacgaggaa cgtggccagg agaaagcgct cgagcagcca gattccgtca
     2221 aggatccctt ggtgtgagaa accgatgccg ctcgcgttgt agaagaacga tcgcgccggc
     2281 cgcaaaaggc tgaagccgac ctggttgctg cctgacagcg taggcagctt tccaccccag
     2341 acctcgcgca cgtggaactg ccacgagacc tcgacggccg cgggtacgca gagcgcaacg
     2401 acctgcgcga cgccggtgcg ccgacggatc agcccgccgc cgcgcagtac tcgcacgagg
     2461 caccacaccc ccatgccgag cagcacgatc aggaccgtct cgcggcacaa gaccgcggca
     2521 gtgaacgcca tcgacgccag ccaccacttg cgtcgcgtcc aggcaaggag accgatcacc
     2581 agcgctgcgg tcgcgagagg ttcggtcagg tcgcgtgcga gcgccatgac gagtgcagga
     2641 gaagccgcaa gagccagacc ccaccacgtc gaccgaccgt attgtctcgc ccacaccgca
     2701 gccgcccagc cgacagcgac gagggcgagc acgttcacga gcacaagggt gagggacgtc
     2761 ggtgcgcgga acacctcccg tacgaaccac gcggtggcgg gcaggccgat tcgctgttgc
     2821 cggtatggcg ggttgtccaa cgtgatgtga aacgcagtgg cccgatgtgt caacggatcc
     2881 aacgccaggc ggtagtagaa ctgcccgtcg tatccgttgg tgccggtgtg cacagggagg
     2941 tcggcacttg cgccggggtg gacgaagacc gagctcgcca aagcgaacgc accgagatga
     3001 gcgccgtgcg tgagatcgcg cgccgcgacg aacagcacag ccagtgcacc ggcaacggcg
     3061 gcgaccacgc cgggtgccgg agccgcatcg agtcgacggt cgagtgccct gatggtccgg
     3121 actttagccg agatgctgga cctcgaccgg tcgcgacgcg gtcggcgaac ccggatccgt
     3181 caggaatgcg tcagggtttt gcgggcccgc gtcagggttc catcaagaaa cgcgacgttc
     3241 agcgtgctca caggttcact cgaatcagtt gccgccccgg caccgattcg aaaggaggcc
     3301 gtcgtgctga acgtcatgca cgagcggtcg acggggttcg aaccccggcg cgagactgtc
     3361 gctgctgcgg atccccgatt gacagacccc ttcgtcggca tggtggcctt tggcgcgacc
     3421 ctggccgtgg ctgtcgcact cgccagagcg ggctccgccg tcggctgggg cacccagcta
     3481 gcggcgatgg cggtgacggt cgcactcgtc tgctggtggt gccgcgtgct gccgagcctc
     3541 tttgtggcgc tgctcggttg gctgatgctc aacggcatcg tcgtcaacgg ggccggccgc
     3601 cttggctggg atggccatca agatctcatc cgcatcggtg tgctgctcgg tactgggttc
     3661 ggcgtggcgc tggccaggag gctcgagttg cagctgcgct cggtcccaac cggcagccct
     3721 gccgtagaaa ccgatcggca tctatacgtc gtgcggcccg acaatgaact gcaaggagat
     3781 cgtcatgcgt gacctgttct tcatcctgct caccctcgca gtgttcgcca tctttgcgct
     3841 gatcgcgcgc ggggcggaaa agctgtgagc gccgaaaatc tgatcggtct gattcttgcc
     3901 atcggagtca ccatctacct ggtggtggcg ctgctgttcc cggagcggtt ctaagtgcac
     3961 gctacggctg caggcattct ctttttggtc tcactcgtca ttgcgctggc actgtcgtac
     4021 aggccacttg gcgactacat ggcgcgggtt ttcagcagca agcaccactg gcacgccgag
     4081 cgggtcatct atcgtgcgat tggggtgaac cccgacgccg accagaagtg gtcgacgtat
     4141 ctcagaagca tgttggcatt ctcgctcgtg tcggtgctcg ggctctactt catgctgcgc
     4201 gcgcagcatc atctcccgtt cagccttggc tacaagggca tggagcaggg tcagtcgttc
     4261 aacactgccg ccagcttcgt cacgaacacc aactggcagt cgtactcagg tgaatcgaca
     4321 cttggctaca ccgcgcagat ggtcggcctg gccgtgcaga actttgcatc ggccgcagtg
     4381 ggaatctgtg tggcgattgc gctggtgcgt ggattcgccc gctcgcgtac cgatcaactc
     4441 ggcaacttct gggtcgacct gatccgtacg gtcacccgca tcttgcttcc gatctccgtc
     4501 gtattcgcgg tgattctcct tgcgggcggc gcgatcgaca acttccacgc cacgcacgtg
     4561 gtgaacacca tcgccggcag ccaccagtcg atcaccggtg gaccgattgc atcgcaagag
     4621 gccatcaagc agctcggcac caacggtggt ggcccttaca acgcgaactc ttcgcacccg
     4681 ttcgagaatc cgaccaactg gaccaactgg ttccagatct acttccttct cgtcatcgcg
     4741 ttctcgttgc cgcgcacgtt cggcaagatg gtcggcgaca agcgtcaggg gctcgcgatc
     4801 gtcgccgtaa tggcgatcct gtcgatcctg agcgtgatcg gtctcaacgt ggcgcaaggt
     4861 gtgcaccacg gcagcgtgcc tacctcggtc ggcgccgcgt cagaaggaac tgagacacgg
     4921 ttcggtgtgc tcgactccgc gacgttcgct tccgcgacga cactgacgtc aacaggtgct
     4981 gttgactcgc tgcacgactc ctacaccagt ctcggtggcg cgatcacgct gctcgacatt
     5041 cagttgggcg aggtcgcgcc tggcggtacg ggcagtggcc tctacgggat tttggtcctc
     5101 gctgtcatca ccgtctttgt ggcaggtttg atggtcgggc gaacaccgga gtatctgggc
     5161 aagaagattg catcgcgaga gatgaagttc tcgtccctgt acttcctgac gactccggcg
     5221 ttcgtgctga ttggcacggc agtcgcgatg g
//
LOCUS       DASWVF010000275         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_466210, whole genome shotgun sequence.
ACCESSION   DASWVF010000275 DASWVF010000000
VERSION     DASWVF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466210"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGD55_04880"
     CDS             complement(<1..510)
                     /locus_tag="VGD55_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0869709.1"
                     /translation="MEIDVLGPVVVAGSDARLGHRDRVILSALALEPGEVLSPESLAD
                     ALWGETPPKSWPKVVQGSMMRLRRALGPHAIETTGTGYRLSVPDDDVDHVRFERLVAR
                     ARGFATVREPARAVTTFELALALWRGPPFSELDGWDAGRAEAARLTEVRRASEEELVE
                     ARLAAGRPVE"
     gene            682..1197
                     /locus_tag="VGD55_04885"
     CDS             682..1197
                     /locus_tag="VGD55_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0869710.1"
                     /translation="MNKLSRRWIAAGAALAAAIGVAVASSPVQATPSTAATTVLAHAP
                     VEPLHAVAVNTTISGQPWAALVRTYGVTDGYVVENDFQPGQASGWHSHPGPSLVFVVA
                     GSITNYTSDQPHCKGVTYAAGTTFVDAGGTDVHELVNAGTIPAETIAVQLIPQGQPRR
                     IDEPEPSNCHV"
     gene            complement(1265..1645)
                     /locus_tag="VGD55_04890"
     CDS             complement(1265..1645)
                     /locus_tag="VGD55_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0869711.1"
                     /translation="MPDLERNKAVVVDYYQTAFNGEPEKAAAEHLGSRYVQHNPAAPD
                     GPEAFIGFVHWLRGEYPQLQLDIKRVIAEGDIVVTHAQLILTPGEPGQALADFFRVED
                     GKVVEHWDVVQDIPATSANSNTMF"
     gene            complement(1674..2195)
                     /gene="msrA"
                     /locus_tag="VGD55_04895"
     CDS             complement(1674..2195)
                     /gene="msrA"
                     /locus_tag="VGD55_04895"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0869712.1"
                     /translation="MAEMTERAVLAGGCFWGMQDLIRRRPGVISTRVGYSGGDVANAT
                     YRKHGNHAEAVEIIFDPTQTSYRDILEFFFQVHDPTTRNRQGNDTGASYRSAIYYADD
                     EQKRVALDTIADVNASGLWPGDVVTEVEPVGPFWEAEPEHQDYLERYPDGYTCHYPRP
                     NWKLPKRSEAVSS"
     gene            2376..2744
                     /locus_tag="VGD55_04900"
     CDS             2376..2744
                     /locus_tag="VGD55_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0869713.1"
                     /translation="MQASVGYLAIDSTDPERLTRFWCAVLGVEVDSTIGDGQFIVLSP
                     TKDGLTVGFQRVPEEKSGKNRVHLDLVVEDLDAATQEIEALGGHWLEPGNTRELEGFQ
                     WRIMADPEGNEFDIDLMPPE"
BASE COUNT          506 a          950 c          883 g          471 t
ORIGIN      
        1 ttcgaccggg cggccggcgg ccagccgcgc ctccacgagt tcttcctctg atgcacggcg
       61 cacctccgtg agccgggcgg cttccgcccg acccgcatcc cagccgtcaa gctcagaaaa
      121 ggggggtccg cgccacaatg caagcgccag ctcgaaggtc gtgaccgcgc gcgccggttc
      181 acgaacagtc gcgaatcccc gcgcgcgggc caccagtcgc tcgaagcgga catggtcgac
      241 gtcgtcgtcg ggcaccgaca ggcggtaacc cgtgcccgtt gtttcgatgg catgcggacc
      301 gagcgcccga cgcaaccgca tcatcgagcc ctgtaccacc ttcggccagg acttgggtgg
      361 ggtctcgccc cacaatgcgt cggccaagct ctctggtgag agcacctcac cgggctcgag
      421 cgcgagcgcg gacaagatca cgcgatcgcg atggcccagg cgcgcgtcac tgccggcaac
      481 gacgacgggg ccgagtacgt cgatctccat gttgggagtg tcgaagctgg cacccgaccg
      541 gtcaatgggc gtgataccgc cgtgataccg gctcggcggc gatgtgacac cgcgtgtctg
      601 cagggtggcc gcatcagctc ccgtttgagg agctcacgac aagagggggt caacgtgttt
      661 gcgtaccaga gaggacccgt catgaacaag ctaagtcggc ggtggattgc ggccggcgcg
      721 gcgctagccg cggccatcgg cgtcgcggtg gcaagctcgc cggtacaagc caccccgagt
      781 acggcggcaa cgactgtgct ggcccacgcg cctgtcgaac cgttgcacgc cgtcgcagtc
      841 aacacaacga tcagcggaca accctgggcg gcgctggtca ggacgtacgg agtgaccgac
      901 ggctatgtag tcgagaacga tttccagccc ggtcaagcgt caggatggca ttcgcaccct
      961 ggaccgagcc tggttttcgt ggtcgcagga tccatcacca actacaccag tgaccagccg
     1021 cattgcaaag gcgtcacata cgcggctggc accacatttg tcgacgccgg cgggacggac
     1081 gtccacgagc tggtgaacgc cggcacgatc ccggctgaga ccatcgccgt gcagctcatt
     1141 ccgcaggggc agccacgacg gatcgacgaa cccgagccat ccaactgtca cgtgtgacga
     1201 cgccctcggt gcggctggcc tgccttgcgc aggccagcca agccgaccac atctacgccg
     1261 acgctcagaa catcgtgttc gagttcgccg aggtcgcggg aatgtcctgg accacgtccc
     1321 agtgctcgac gaccttgccg tcctcgacgc ggaagaaatc agcaagcgcc tgacccggct
     1381 cgcccggcgt caggatcagc tgggcatggg tcacgacaat gtcgccttcc gcgatgacgc
     1441 gcttaatgtc caactgcagc tgcgggtact ccccacgaag ccagtgcacg aacccgatga
     1501 aggcctcggg tccgtccggc gctgccgggt tatgctgcac gtaccgactc cccaagtgct
     1561 ccgcagccgc cttctccggc tcgccgttga acgccgtctg gtagtaatcg acgacgacag
     1621 ctttgttgcg ctcgaggtcg ggcatcgcat ttcccatcgt cggtggagct tgcttacgaa
     1681 ctcacggctt cgctgcgctt cgggagcttc cagttcggcc gcggatagtg gcaggtgtag
     1741 ccgtcggggt acctctccag gtagtcctgg tgctccggct cggcctccca gaacggcccc
     1801 accggctcga cctcggtgac cacgtcgccc ggccacaggc ctgaggcgtt gacgtcggcg
     1861 atcgtgtcga gcgcgacccg cttctgctcg tcgtccgcgt aataaatggc cgaccgatag
     1921 ctggcgccgg tgtcgttgcc ctgacggtta cgtgtcgtcg ggtcgtgcac ctggaagaag
     1981 aactcgagaa tgtcgcggta ggaggtctgg gtcggatcga agatgatctc gaccgcctcc
     2041 gcatgattcc catgtttgcg gtaggtcgcg ttcgccacgt cgccaccgct gtacccgacc
     2101 cgggtcgaga tcacgccggg acgccgacgg atgaggtcct gcattcccca aaagcacccg
     2161 cccgcaagga ccgctcgctc ggtcatctca gccatcgtca tactcctcgt cgtccgccaa
     2221 cttgaaggtg cttctcgcct acgctccaac gctacccggc ggtgtctatg cccgcgaggt
     2281 tcgatcgctt acggtccgag gacggctgcg acagcggcgc cgctggcccg atctacgatg
     2341 cagccagcag acacccatac ataggagcga ttgcgatgca ggcttcggtc ggatacctcg
     2401 ccatcgacag caccgaccca gagcgactga cacgattttg gtgcgcggtg ctcggcgtcg
     2461 aggtcgactc cactatcggc gacggtcagt tcatcgtgct gtcgccgacc aaggacggtc
     2521 tgaccgtggg tttccagcgc gtccccgaag agaagtcggg caagaaccgt gtgcatctcg
     2581 acctggtcgt cgaggatctc gacgccgcga cacaggagat cgaagcgctc ggcgggcact
     2641 ggctcgagcc aggcaacacc cgcgagctcg agggcttcca gtggcggatc atggccgacc
     2701 ccgagggcaa cgagttcgac atcgatctca tgcccccgga gtaacgccaa gcgaaaatca
     2761 gccgcccaga tacgacatct cagcccgcgg cagatccttg gccgaccgct
//
LOCUS       DASWVF010000276         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_135044, whole genome shotgun sequence.
ACCESSION   DASWVF010000276 DASWVF010000000
VERSION     DASWVF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135044"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGD55_04905"
     CDS             complement(<1..339)
                     /locus_tag="VGD55_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0869714.1"
                     /translation="MDSPEEHDVDLLIVGAGPAGLYAAYYAGFRALSVVVVDSLTEPG
                     GQLAALYPEKMIYDVGGIPAILGRELAADLFRQAQTYKPTFLLGHGADQLTTLDDGTL
                     QVTTDHGHTIH"
     gene            406..1101
                     /locus_tag="VGD55_04910"
     CDS             406..1101
                     /locus_tag="VGD55_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0869715.1"
                     /translation="MSGPPLLARRGVLAAIAVAAFGVMLVIGLRYSGTHGPGRLDRAI
                     DIRIHNRLGYHPVLLQRVVNLANPGSAVAICAVLCVVFFLMARRRLAVLVVIGPALSG
                     VLVDVVLKPLFDRRLAGALSYPSGHTAAASSIALVVVVAMLGGSRMSWPAAVRWVVAA
                     CALAAAGLVAAALIAEGYHYTTDTFGGFLVAVTCVLGAALSIDALASRPHDESKGEST
                     PQVDDALLPRVKA"
     gene            1098..1730
                     /locus_tag="VGD55_04915"
     CDS             1098..1730
                     /locus_tag="VGD55_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEY0869716.1"
                     /translation="MTHLAATERASLSDLLDELGPFAPTLCEGWRTRDLTAHLVLRES
                     RLDTLAGVVIGALASRTARVQRKVAHRNFSELVNRLRTGPPWWSLFRLPRVDEGANNL
                     EFFVHHEDVRRAQPDWKPRVLDAATDELLWRRCRRIARLALRRAPGGIELLRSDTGER
                     HTGRAPAQGRSVLTLSGPPQELLLYLYGRRDHALVESDGDAETLRVLEKS"
BASE COUNT          285 a          643 c          654 g          340 t
ORIGIN      
        1 gtggatcgtg tgaccgtggt cggtcgtcac ctgaagcgtt ccgtcgtcga gcgtggtgag
       61 ttggtcggcg ccgtgaccga gcaggaacgt gggtttgtaa gtctgggcct gacggaacag
      121 gtcggccgcg agttcgcgac caagaatcgc cggaatcccg ccgacgtcgt aaatcatctt
      181 ctcggggtac aaggccgcga gctgcccgcc aggctcggtc agcgagtcga cgacgactac
      241 agacagcgcc cgaaatcccg cgtaataggc cgcatataac ccggctggcc cggcaccgac
      301 gatgagcagg tcgacgtcgt gctcttcagg ggaatccaca acctgcacgc tagtggatat
      361 cgccggacgc cagcggtagc gcaccgcggc tcgtagactt gacgaatgtc tggcccaccc
      421 ctgctagcgc gccgcggcgt gctcgccgcc attgcggtcg ccgcctttgg ggtaatgctc
      481 gtcatcggac tgcggtattc gggcactcac gggccgggtc gtctcgaccg cgcgatcgac
      541 atccgcatcc ataaccgcct cggttaccac cccgtcttac tccagcgcgt cgtcaacctc
      601 gccaaccccg gcagcgccgt cgcgatctgc gcggtgctgt gcgttgtgtt cttcctcatg
      661 gcccgccgcc ggctcgccgt cctggtcgtc atcgggccgg cgctgtccgg cgtcctcgtc
      721 gatgttgtct tgaagccgct gttcgatcga cgtcttgctg gcgcgctgtc gtatccgagc
      781 ggacacaccg cggccgcgtc gtcgatcgct ctcgtcgtgg tcgtggcgat gctcggcggg
      841 agtcgcatgt cgtggcccgc tgcggtgcgc tgggtcgttg ctgcgtgcgc gctggctgcg
      901 gccggcttgg tcgccgccgc gctgatcgcc gagggctacc actacacgac cgacacgttc
      961 ggcgggttcc tcgtcgcggt cacctgcgtc ctgggggccg cgttatcgat cgacgcgctc
     1021 gcatcgaggc ctcatgatga gagcaagggc gagagcaccc cgcaggtgga cgacgcgttg
     1081 ctgcctagag tgaaggcgtg acccatctcg cagcgaccga acgagccagc ctcagtgacc
     1141 ttctcgatga gcttggtccg ttcgcgccga cgttgtgcga gggctggcgt acccgcgatc
     1201 tgaccgcgca cctcgtgttg cgtgagagca ggctcgacac gctcgctggc gtcgtcatcg
     1261 gtgcgctcgc cagccgaacc gcgcgggtac agcgcaaggt cgcgcaccgc aacttcagcg
     1321 aactggtgaa ccggctgcgc accgggccgc cgtggtggtc gctgttccgg ttgccgcgcg
     1381 tcgacgaagg cgccaacaac ctcgagttct tcgtgcacca cgaagatgtg cgccgcgcac
     1441 aacccgactg gaagccgagg gtgctcgacg cggcgaccga cgagctgctg tggcgacgct
     1501 gccggcgcat cgcccgactc gctttgcgtc gggcacccgg tggcatcgaa ctgctgcgct
     1561 ccgacaccgg cgagcggcac accggccgag caccggcgca aggccggagc gtgctgacct
     1621 tgagtggccc gccgcaggag ctactgcttt acctctacgg acgccgcgat cacgcgctgg
     1681 tcgagtccga cggggacgca gagacgctgc gggtgctcga gaagtcttga gtctcgggtt
     1741 ctgcgacgtg acacgccgcc gtgtgcccgg ggccgagcaa tcgcagttgc ggagtgacgt
     1801 gatggcactc ctcgataacc agcgggcaac gtgcgcggaa gcggcaacca ggtgtcgggt
     1861 cgatgacctt cggcggttcg cccttgtcgg tttcggcacc gaccgcgagc ggagcccgcg
     1921 ga
//
LOCUS       DASWVF010000277         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_211545, whole genome shotgun sequence.
ACCESSION   DASWVF010000277 DASWVF010000000
VERSION     DASWVF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211545"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /gene="arc"
                     /locus_tag="VGD55_04920"
     CDS             complement(<1..1654)
                     /gene="arc"
                     /locus_tag="VGD55_04920"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEY0869717.1"
                     /translation="MASREDSRDGQTNDSRAQGDDDAAQLQSQLGLLEEEISMLRRKL
                     SESPRSARVLEERLADAQSTMAGLTGQNERLVSTLKEAREQIIALREEVDRLAQPPSG
                     YGVFLARHDDGTVDIFTGGRKLRVTVSPAVEAEGLRKGQEVMLNEALNVVAALAFERQ
                     GEVVMFKEVLEDGERALVIGHSDEERVVMLADTLLDTPLRVGDSLLLETRSGYVYERI
                     PKAEVEELVLEEVPDIDYSAIGGLAAQIEMIRDAIELPYLHADLFREHELRPPKGVLL
                     YGPPGCGKTLIAKAVANSLAKKVAEVTGKPEGKSYFLNIKGPELLNKYVGETERKIRL
                     VFQRAREKASDGNPVIVFFDEMDSIFRTRGSGVSSDVELTIVPQLLSEIDGVEGLENV
                     IVIGASNREDMIDPAILRPGRLDVKIKIERPDAEAARDIFSKYIKAHLPLHAEDLAEH
                     GGSREATVQAMIMRTVERMYDETEENRFLEVTYANGDKEILYFKDFNSGAMIQNIVDR
                     AKKMAIKDFIESGNKGLRMQHLMSACVDEFKENEDLPNTTNPD"
     gene            1799..2071
                     /locus_tag="VGD55_04925"
     CDS             1799..2071
                     /locus_tag="VGD55_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0869718.1"
                     /translation="MVGHRVWIDQDLCTGDGICVQEARNVFEFDIDGLAYVKNAAGDL
                     LTEVAAQTDVPPDTFKEVVEAARKCPGACIHVVRAIDGVEVAGPDA"
     gene            complement(2068..2964)
                     /locus_tag="VGD55_04930"
     CDS             complement(2068..2964)
                     /locus_tag="VGD55_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY0869719.1"
                     /translation="MTSLIKRGPFEVGDQVQITDPKGRKHTVVLSPGKQFHTHQGALE
                     HDELIGRPEGVVVTSTGGTPYLAFRPLLSDFVLSMPRGAAVVYPKDAAMIVGYADVFP
                     GARVIEAGAGSGALSCSLLRAVGETGLVYSYERREDFAAVAVKNVERFFGSAHPAWRL
                     SVGDLVESLAEHPPDEPVDRVVLDMLSPWECLDAVARVLTPGGLVCCYVATTTQLSRV
                     VEALREDGRFAEPASWETMLRTWHVEGLAVRPDHRMIGHTGFLVTARRLADGVVAPPR
                     RRRPTTTGSAEAAGSGESASPG"
     gene            complement(2961..>3461)
                     /locus_tag="VGD55_04935"
     CDS             complement(2961..>3461)
                     /locus_tag="VGD55_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0869720.1"
                     /translation="GAHPSGSAISTLWAFVIAATIWVSSTQVLRQQALRDRLPGISAR
                     ALSRSSISVSADLPLAEAVRRAQEAHAQGIVIVDDDGRPTGVVKEAAVTAMPAPRRPW
                     VSAGSVARHISAGELIDASWSGEELLVKLQLAAASEYVVVDPTGGIYGVLAAADVARA
                     LAGSAA"
BASE COUNT          579 a         1136 c         1128 g          618 t
ORIGIN      
        1 cgtcggggtt ggtcgtgttc gggaggtctt cgttctcctt gaactcatcg acgcatgctg
       61 acatgaggtg ctgcatgcgc aggcccttgt tgccgctctc gatgaagtct ttgatcgcca
      121 tcttcttcgc gcggtcgacg atgttctgaa tcatcgcgcc ggagttgaag tccttgaagt
      181 acaggatctc cttgtcgcca ttggcgtagg tgacctcaag gaatcggttc tcttcggtct
      241 cgtcgtacat ccgctcgacc gtgcgcatga tcatggcctg cacagtggct tcgcgtgaac
      301 cgccgtgctc ggccaaatct tcagcgtgca aaggcaaatg tgccttgatg tacttgctga
      361 agatgtcgcg cgccgcctcg gcgtcgggcc tctcgatttt gattttgacg tcaagccgac
      421 cgggccgaag gatcgctggg tcaatcatgt cttcgcggtt cgacgctccg atgacgatga
      481 cgttctcaag gccttcgacg ccgtcgatct cactgagaag ttggggcacg atcgtgagtt
      541 cgacatcgga actcacaccc gaaccccggg tgcggaaaat cgaatccatc tcgtcgaaga
      601 acacgatgac cgggttgccg tcgctcgcct tttcgcgtgc ccgctggaaa acaaggcgga
      661 tcttgcgttc ggtctcgccg acgtacttgt tcagcagctc agggcccttg atgttgagga
      721 agtagctttt gccctcgggc ttgccggtga cctcggcgac cttcttcgca agcgagttcg
      781 caacggcctt cgcgatgagg gtcttgccgc agccgggcgg cccatagagc aatacgccct
      841 tgggcggtcg aagctcgtgt tcgcggaaca ggtcggcgtg caggtagggc aactcgatgg
      901 cgtcgcggat catctcgatc tgcgccgcca acccgccgat cgcggagtag tcgatgtcgg
      961 gcacctcttc gaggacaagc tcctcgacct cggccttggg gatgcgctcg tagacgtagc
     1021 cggagcgtgt ctcgagcagg agtgagtcac cgacccgaag cggcgtgtcg agcagcgtgt
     1081 cggcaagcat cacgacgcgt tcctcgtcgc tgtggccgat gaccagggcg cgctcgccgt
     1141 cctcgagaac ctctttgaac atgacgactt cgccctggcg ttcgaatgcg agcgcggcga
     1201 cgacgttgag ggcctcgttg agcatgactt cctggccctt gcgaagcccc tcggcttcaa
     1261 ccgcaggact caccgtgacc cgcaacttcc ggccgccggt gaagatgtcg acggtcccgt
     1321 cgtcgtggcg agccaagaag acgccatagc cggacggcgg ttgggcgagg cggtcgacct
     1381 cttcccgcag cgcgatgatc tgctcgcgag cctctttcag ggtgctgacg aggcgctcgt
     1441 tttgaccggt gaggccggcc atcgtcgact gcgcgtcggc gagccgctct tcgagtaccc
     1501 gcgcgctgcg tggcgactcg gacagcttgc gccgcagcat cgagatctcc tcttcgagga
     1561 gtccgagctg cgactgcaac tgcgccgcgt cgtcatcacc ctgcgcgcgc gagtcgttcg
     1621 tctgaccatc gcgagagtcc tcacgactag ccacgagcct cacctccctg caggactgca
     1681 cctcgaccct accgtcggtg caggcgcact gcgcttcgga cgccttgtta tcgtcggttg
     1741 tggcgcggac ctgatagttc gcgcgccacg acgtgaaaca ggaccgggag gtgggtttat
     1801 ggtcgggcac cgagtctgga tcgaccagga cctctgcacc ggcgacggga tctgcgtcca
     1861 ggaggcgcgg aacgttttcg agttcgacat cgacggcctg gcgtacgtca agaacgcagc
     1921 aggcgacctg ttgaccgagg tggcagcaca aaccgacgtc ccgcctgaca cgtttaaaga
     1981 ggtagtcgag gccgcccgga agtgtccagg ggcgtgcatc cacgtcgtcc gggccatcga
     2041 cggcgtcgaa gtcgccggac ccgacgccta gcccgggcta gccgattcgc ccgacccggc
     2101 tgcttcggcc gacccagttg tggtgggtcg acgccgccgc ggcggtgcaa cgaccccgtc
     2161 ggcgaggcga cgcgcggtga ccaggaagcc ggtgtgaccg atcatccggt ggtcggggcg
     2221 caccgccaga ccctcgacgt gccaggtgcg cagcatcgtc tcccacgacg cgggttcggc
     2281 gaaccggccg tcttcgcgca gcgcctccac aacccgtgac agctgggtcg tcgtggcgac
     2341 gtagcagcag accaggccgc caggggtcaa cacccgcgcg acggcgtcga ggcactccca
     2401 gggcgacagc atgtcgagga cgacgcggtc gaccggctcg tcgggtgggt gctcggcgag
     2461 tgattccacg aggtcgccga cgctgagccg ccatgccggg tgcgccgagc cgaagaagcg
     2521 ctcgacgttc ttgaccgcca ccgccgcgaa gtcttcgcgc cgctcgtagg agtagacgag
     2581 gccggtctcc cccaccgcgc gtagcagcga gcaactgagg gcaccggatc cggcaccggc
     2641 ctcgatcacc ctcgcaccgg ggaagacgtc ggcatacccg acgatcatcg cggcgtcctt
     2701 ggggtagacg accgccgcac cccgcggcat cgacagcacg aagtcagaca gcaatggccg
     2761 aaacgcgagg tacggggtgc ccccggtcga cgttacgacc acgccctcag ggcggccgat
     2821 cagctcgtcg tgctccagcg ccccttggtg agtatggaac tgcttgcccg gtgagagcac
     2881 caccgtgtgc ttgcgaccct tcgggtcggt gatctgcacc tggtcgccga cctcgaacgg
     2941 tccgcgtttg atcaacgacg tcatgccgcg ctgccagcga gtgcccgggc cacgtcggcg
     3001 gccgcgagaa ctccataaat gccgcccgtc ggatcgacca cgacgtactc cgatgcggcg
     3061 gcgagctgga gcttgacgag gagttcctct cccgaccacg acgcgtcgat cagttcaccc
     3121 gccgagatgt ggcgagcgac agaaccagcg cttacccatg gacggcgcgg ggccggcatc
     3181 gctgttacgg cggcctcctt cacaacgccg gtagggcgcc cgtcgtcgtc gacgatcacg
     3241 atgccctggg cgtgggcctc ctgtgcgcgc cggacggctt cggcgagcgg caagtcggcc
     3301 gacaccgaga tcgaactgcg actcagtgct cgagcggaga tgccaggcag tcggtcgcgc
     3361 aaagcctgct ggcgcagcac ctgggtcgag ctgacccaga tcgtcgccgc gatcacgaac
     3421 gcccacaacg tgctgatcgc ggaaccgctc ggatgcgcgc c
//
LOCUS       DASWVF010000278         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_32973, whole genome shotgun sequence.
ACCESSION   DASWVF010000278 DASWVF010000000
VERSION     DASWVF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32973"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGD55_04940"
     CDS             complement(<1..204)
                     /locus_tag="VGD55_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY0869721.1"
                     /translation="MSSPYVGEIRLFAGNFAPQGWMTCDGQVLAIADNDVLFNLIGTT
                     YGGDGQNTFALPRLSGCVPLHMGT"
     gene            complement(224..2350)
                     /locus_tag="VGD55_04945"
     CDS             complement(224..2350)
                     /locus_tag="VGD55_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869722.1"
                     /translation="MLSMKHNSRLVASLLAIATSISLAACGGGGHSNAPVLPPPQTPA
                     STYNGPLADATFTITIPVPATSSSTKRRPAYVSSSTSKIVFTLNSTTNPNITSAQVTA
                     FNSSQLGSKPVTLNSAQCPGTGPWTCTIPIKLPPGGDNLTVSAQDASSNILSQQVKTL
                     TVVAGAANSFSMTLDANAATMTITATDGFCAGTVAVTASQTLGTVGTAPITFAATYTD
                     PAGKTIVAPGLPLLTVNGHTDDNGGAGYTDGTTHLNVKVTQSAQTFTLTQTAGTGNAA
                     VSVAATPPASDNLSFNKTLSFTLSSGPAIPSSFVAAIEHTGSATGKIDFFTLTLGASS
                     DTFSAYSTPTLSDQSNDVDFPQDLLFDGNGDLLIANGGAGNPDFGNFACVPAGAISTG
                     ANVATVLTTNVDDPAFIALGSDGSVGLGNSVANSTYDFEEFILSGTYVAAATTRDIAR
                     SGAVATQGVVALPATPANPSGSYAVAITDGTQANSKVVIKHPDGTSVTITDAHMYDPF
                     IAYDPSNSQLVVANDGRGSTSGNFQLSNVQFYTVGTTPVKVKEIVLDDDGTGNAAAAP
                     YIVSASSTGLVAVGIISEDNNAEVVLYNGTSSRGEIAAPIPYDATTTNDFSYSTFVIG
                     NSGNTVTAVTGLRWLTGTKLMVSLEAEISNSPVSGWNGLHTYDTTQTQANSGYEPVYQ
                     NAEGASAKQTGFQSLSSLPSAVAYKP"
     gene            complement(2361..>3279)
                     /locus_tag="VGD55_04950"
     CDS             complement(2361..>3279)
                     /locus_tag="VGD55_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869723.1"
                     /translation="ISAGSCNSGCTVNGPPSPPGVLDTYSLVTYDGSNGTGNALDTGT
                     ASFTPIVGQANSTSVTMKGIPATIVINGVPNNFAAGTAGQGTALSVTVTDADGRVITG
                     TYANPITINAPDANSRGVTLTGTNPGCAGHCATLNGSTDSVSLLYGGVAENPITLTSA
                     ATGVSGPNAGSAIFTPVLQPIVWFSGPTSVVAGSPKGVDLFTNDNTSAIGYTGTEVYA
                     ESGFTNSPYSMALSVDGASACSTFATVTTGADTSNGTPFTATSIASPVAGLCTLTVTD
                     NLTDQPNALPTFVVTYTTSSVGADGKRRR"
BASE COUNT          514 a          978 c         1126 g          661 t
ORIGIN      
        1 cgtgcccatg tgcagcggga cgcaccccga gagcctcggc agcgcgaacg tgttttgccc
       61 gtcaccgccg taggtcgtgc cgatcaaatt gaacagcacg tcattatcgg cgattgcaag
      121 cacctgcccg tcgcacgtca tccatccctg cggggcgaag tttcccgcga acaggcgaat
      181 ctcgccgaca taaggactcg acatcgtctc tcctctctcc cggttacggc ttgtaggcca
      241 cggccgaggg caggctgctc aaactctgga aacccgtctg cttggcggat gcgccttcgg
      301 cgttctggta cacgggttcg tagccggaat tggcctgcgt ctgcgtcgta tcgtaggtat
      361 gcaggccatt ccagccgctg accggcgaat tgctgatctc ggcctccagc gacaccatca
      421 gcttcgttcc ggtaagccaa cgcagacccg tcacggcggt gaccgtgttc ccgctgttgc
      481 cgatgacgaa ggtgctgtag ctgaagtcgt tcgtcgtcgt ggcgtcgtac ggaatcggcg
      541 cggcgatctc gccccgcgac gaggtaccgt tgtagaggac gacttcggcg ttgttgtcct
      601 cactgatgat cccgacggcg accagtccgg tcgacgatgc cgaaacgatg tacggggcgg
      661 cggcggcgtt gccggttcca tcgtcgtcga gcacgatctc cttgaccttt accggcgtcg
      721 tgccgacggt gtagaactgg acgttcgaga gctggaagtt tccgctcgtc gacccgcgtc
      781 catcattcgc caccaccagc tgactgttgc tcggatcgta agcgatgaac ggatcgtaca
      841 tatgcgcatc cgtgatcgtc acgctcgttc cgtcgggatg cttgatgacg accttggaat
      901 tcgcctgtgt cccgtcggtg atcgccaccg catagctgcc tgaggggttt gctggggtgg
      961 ccgggagcgc gacgacgcct tgggtcgcga cggcgcctga gcgagcgatg tcgcgcgtcg
     1021 tcgcggcggc gacgtaggtg ccgctcagga tgaactcctc gaagtcgtag gtggaattcg
     1081 cgacgctgtt gcccaggccg acactgccgt cggacccaag ggcgatgaac gcggggtcgt
     1141 cgacgttcgt cgtcagcacc gtcgcgacgt tggcgccggt tgaaatcgcg ccggcgggca
     1201 cgcaggcgaa gttgccgaag tcggggttcc cggcgccgcc gttggcgatc agcagatcgc
     1261 cgttgccgtc gaagagcagg tcctgcggga agtcgacgtc gttgctctga tccgagagcg
     1321 tcggcgtcga gtacgccgag aacgtatcgc tgctcgctcc cagcgttagc gtgaagaagt
     1381 cgatcttgcc ggtcgcggag cccgtgtgct cgatcgcggc gacgaagctg gaggggatcg
     1441 ccggtccgct cgagagcgtg aagctcaagg tcttgttgaa gctcaagttg tcggacgccg
     1501 gcggcgtcgc ggcgacactg acggccgcat ttcccgtacc ggccgtttgc gtcagcgtga
     1561 aggtttgggc gctctgagtg accttcacgt tgaggtgcgt tgtgccgtcg gtatagccgg
     1621 ctccgccatt gtcgtcggtg tgcccgttga cggtgagcaa cggtaatccg ggcgcgacga
     1681 tcgttttgcc ggccggatcc gtgtaggtcg ccgcaaacgt gatcggggcc gtcccgacgg
     1741 ttccgagggt ttgacttgcg gtcacggcca ccgtgcctgc gcagaacccg tccgttgcgg
     1801 tgatcgtcat cgtggcggcg ttggcatcga gcgtcatgct gaacgaattg gccgcgcccg
     1861 ctaccacggt cagggtcttc acttgctgcg agaggatgtt gctcgatgcg tcctgcgctg
     1921 agaccgtcag gttgtcgccg cccggcggaa gcttgatcgg gatggtgcag gtccagggac
     1981 cggttcccgg acactgtgcg ctgttgagcg tgaccggctt gctgccgagc tggctagaat
     2041 tgaatgcagt gacctgcgcg gatgtgatgt tggggttggt ggtcgaattc agcgtgaaga
     2101 cgattttcga ggtcgacgac gacacatagg ccggccgccg tttcgtgctg ctcgacgtcg
     2161 ccggtaccgg gatcgtaatc gtaaaggtcg cgtccgccag cggcccgttg taggtgctcg
     2221 ccggagtttg cggcggaggt aacacggggg cattactgtg cccgccgcca ccgcatgcag
     2281 cgagcgagat gctcgtcgcg atcgccagca acgaggcgac caaccggctg ttgtgcttca
     2341 tcgatagcat gtgcacggct ttaccggcgg cgtttcccgt cggctccgac cgacgaggtc
     2401 gtgtaggtta ccacgaaggt tggcaaggcg ttgggttgat cggtgagatt gtcggtgacc
     2461 gtgagcgtac acaggcctgc gacgggtgag gcgatcgagg tcgccgtgaa cggcgtcccg
     2521 ttggacgtat cggcaccggt cgttacggtg gcgaacgtcg agcatgcgga cgcaccgtca
     2581 acgctgagcg ccatgctata tggtgagttc gtgaagccgc tctcggcgta gacctcggtt
     2641 cccgtgtagc cgatcgccga ggtgttatcg ttggtgaaga gatcgacgcc cttcgggctg
     2701 ccggccacca cgctcgtcgg tcccgagaac cacacgatcg gctgcagcac cggcgtgaag
     2761 atggcgctgc cggcgttcgg gccgctgacg ccggtcgcgg cggaagtcaa ggtgatcggg
     2821 ttttcggcaa cgccgccata gagcagcgac acgctatcgg tcgaaccatt gagggtggcg
     2881 caatgacccg cgcagcccgg gttcgttccg gtcagcgtga cgccgcggct gttcgcgtcc
     2941 ggcgcgttta tagttatcgg attcgcgtag gttccggtga tcacccggcc gtcggcgtcg
     3001 gtcaccgtga ccgagagcgc ggtgccctgg cccgccgtac ccgccgcgaa gttgttcggc
     3061 acgccgttga tcacgatcgt tgcggggatg cccttcatcg tgaccgacgt cgaatttgct
     3121 tggccgacga tgggcgtgaa cgacgcagtg ccggtatcga gcgcgttccc ggtgccgttc
     3181 gagccgtcgt acgtgaccag cgagtaggtg tcgagcacgc cgggcggcga cggcgggccg
     3241 tttacggtgc aaccgctatt gcagctgccg gcactgatg
//
LOCUS       DASWVF010000279         4117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_160326, whole genome shotgun sequence.
ACCESSION   DASWVF010000279 DASWVF010000000
VERSION     DASWVF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160326"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGD55_04955"
     CDS             <1..882
                     /locus_tag="VGD55_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869724.1"
                     /translation="EAARLEAERIAAEEAARLEAERLAAEEAERLAAEEAARLEAERL
                     EAEQFSADEAARLEAEAAAQIEVARIAAEQAALLEAERVEVERIDAERFAADKVARLE
                     EERFADGVPSYFDQQLHGEQQAREDFAAAQSAAEQASLAAAQYEAEQAEATRIAAVNQ
                     MMAQASAPTDPAHLASANAMRSESNAMLSDFSRTGATDLNPDGSTDESADAHTEPEPD
                     YESAFQPFAQRDLADTASLLRELSSLGFEDEPAPPSSPSPSSSGSSGGHGAGPSTPRP
                     STTTSAQQKKKRGLFGR"
     gene            914..1351
                     /gene="dtd"
                     /locus_tag="VGD55_04960"
     CDS             914..1351
                     /gene="dtd"
                     /locus_tag="VGD55_04960"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY0869725.1"
                     /translation="MRAVVQRASEARVVIDGETVGVLGRPGLVVLVGVTHSDTPAQAE
                     WIASKVYTLRVLDDEKSCADVDAPLLVISQFTLYADTRKGRRPWWNAAAPADIAEPLV
                     TAVVDALRALGAEVATGRFGADMKLHLVNNGPMTLVVDAPEPS"
     gene            complement(1379..2503)
                     /locus_tag="VGD55_04965"
     CDS             complement(1379..2503)
                     /locus_tag="VGD55_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY0869726.1"
                     /translation="MSAPSDSARASDQNEGYRSPLLQLPGAVATDSEPTPGPTPDLKP
                     TDLGVAAHYGDPLREQRNLVEGVGFVDLSHRAVVRISGADRLTWLNDLTTQRLNTLLP
                     QVGSDALILSPQGHVEHHLQLVDDGEAVWVHVEPDSALVLVSFLTSMRFMLRVEVDDV
                     TPDWALVWEPVREPHPTLVSRVDPQVDEAAGRQVFVPRVDLAAFAASADRTGPPAGVL
                     ALEALRVAAGRPRFGLETDHRTIPHELGWIGSAVQLDKGCYRGQETVARVANLGRPPR
                     RLVRLHLDGSVREQLPALGTDISADAEVIGRITSAAYHYELGPIALGVIKYATPDDVA
                     VLVGTGEAAVPATIDAVVERDSTPRPGQAARAAFRTLGVR"
     gene            complement(2519..2653)
                     /locus_tag="VGD55_04970"
     CDS             complement(2519..2653)
                     /locus_tag="VGD55_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869727.1"
                     /translation="MMTLMALTEDAPMHLLAEGLPLSLLWDLADPTGPHSEDLLAHES
                     "
     gene            3093..3656
                     /locus_tag="VGD55_04975"
     CDS             3093..3656
                     /locus_tag="VGD55_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY0869728.1"
                     /translation="MADAEVLASVLSTTEGIIRGVRPDQLDLPTPCPDYTVGTLVAHM
                     VTWSNTFADGAEQLPPNTDRAEYRADDRAGDLFSAASARTVQAFQGGAADRMIDLGNG
                     PSPGEMLFGLMLMEYIGHGWDLAVATDQPTPFSEAGIGAALATGRNMLKPEYRGPDKS
                     FGDIVEVGDEAPALDQLIGFLGRDPAR"
     gene            complement(3653..>4117)
                     /locus_tag="VGD55_04980"
     CDS             complement(3653..>4117)
                     /locus_tag="VGD55_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEY0869729.1"
                     /translation="SLEPLAFLLGAWRGVGVGGYPTIESFRFGQEIVFGHAGKPVLTY
                     VSRSWVLDDAGNVLRAGAGESGYWRVDAAGGLEIVLAHASGFAEIWIGNLAFGRIEMS
                     TDVVARTATAKEVTAGHRLYGLVDGDLLYAYDMAAVGQPLGPHLSARLTKAS"
BASE COUNT          745 a         1363 c         1383 g          626 t
ORIGIN      
        1 gaagctgcac gcctcgaagc cgaacgcatc gccgctgaag aagctgcgcg ccttgaagcc
       61 gaacgcctag ccgccgagga ggcggagcga ctcgctgctg aagaagctgc acgcctcgaa
      121 gccgaacgtc tcgaagcaga acagttctcc gccgacgagg ctgcgcgcct cgaagctgaa
      181 gcagcagcgc agatcgaagt cgcacgcatt gccgcagaac aggctgcgct tctcgaagca
      241 gaacgtgtcg aggtcgagcg gatcgacgct gagcggttcg ctgccgacaa ggttgcccgc
      301 ctcgaagaag aacgatttgc cgacggcgtc cccagctact tcgaccagca actacacggt
      361 gagcagcagg cccgggagga cttcgcagcc gcacaatctg ccgccgaaca agccagtctc
      421 gcggccgcgc aatacgaggc cgaacaagcc gaagcgaccc ggatcgcagc cgtcaatcag
      481 atgatggcgc aggcatccgc gccgaccgat ccagcgcacc ttgcttcggc gaacgcgatg
      541 cggtcggagt cgaacgcgat gctgtccgac ttcagccgca cgggtgcgac agacctgaac
      601 ccggatgggt cgaccgacga gtcagccgat gcgcacaccg agccagaacc cgattatgag
      661 tcggcattcc agcccttcgc gcagcgcgac ctcgccgaca ccgcgtcgtt gctgcgcgag
      721 ttgtcgagcc ttggattcga ggacgagccg gcaccgccga gcagtccgag tcccagttcg
      781 tcgggctcgt cgggtgggca tggagccgga ccaagtaccc cgaggccgtc gacaacaacg
      841 tcggcccagc agaagaagaa gcgcggtctg ttcggccgct aatcggggtc ccttcgcatg
      901 tcaggatgca cgcgtgcggg cagtggtgca gcgggcgagc gaagcacggg tcgtcatcga
      961 tggcgagacc gtcggcgtcc tcggtcggcc gggcctcgtc gtcctcgttg gtgtcacgca
     1021 ctccgataca ccggctcagg cagagtggat cgcgtcgaag gtgtacacgt tgcgcgttct
     1081 cgacgacgaa aaatcgtgcg cggacgtcga tgcgccgctg ctggtgatca gccagttcac
     1141 cctttatgca gacacccgca agggtcgccg accatggtgg aacgcggccg ctcctgccga
     1201 catcgccgaa ccgctcgtca ccgcggtcgt cgacgcattg cgagccctgg gtgcagaagt
     1261 ggccaccggc cggttcgggg ccgacatgaa attgcacctg gtcaacaacg gcccgatgac
     1321 gttggtcgtc gatgccccgg agccatcatg acgaaacgtg accaagcgcc acgaaccgct
     1381 agcgaacgcc caacgtcctg aacgcggcgc gcgctgcctg ccccggccgg ggggtcgaat
     1441 cacgctcgac caccgcgtca atggttgccg gcaccgcggc ctctccggtt ccgaccagca
     1501 ccgcgacgtc gtcgggtgtc gcgtacttga tcacacccag agcgatcggt ccgagctcgt
     1561 agtgatacgc cgcgctggtg atgcggccga tcacctcggc gtccgcgctg atgtccgttc
     1621 caagagctgg cagctgctcg cgcacgctgc catcgaggtg tagccgtacg aggcgtcgcg
     1681 gcggccgacc gaggttcgca acccgcgcca ccgtctcttg cccgcggtag caacccttgt
     1741 cgagttgcac ggcagaaccg atccagccca gctcgtgcgg gatcgtgcgg tggtcagtct
     1801 cgagcccgaa tcgcgggcgt ccggcagcca ctcgcaaagc ctcgagggcg aggacgccgg
     1861 cgggtgggcc ggtgcggtca gctgaggcag cgaacgcggc aagatcgacg cgcggcacga
     1921 agacctgccg cccggccgcc tcgtcgacct gcggatcgac cctgctcacc agggtcggat
     1981 gcggctcgcg caccggctcc cacacgaggg cccagtcggg cgtcacgtcg tcgacctcaa
     2041 cccgcagcat gaagcgcatg gacgtgagga agctgacgag cacaagcgcg ctgtcaggtt
     2101 cgacgtggac ccagaccgcc tcgccatcgt cgacgagctg gagatggtgc tcaacgtggc
     2161 cttgcgggct caagatcaat gcgtcggagc ccacctgcgg cagcagcgtg ttgaggcgct
     2221 gcgtcgtgag gtcgttcagc caggtcagac ggtcggcgcc gctgatccgc acgaccgcgc
     2281 ggtgcgacag gtcgacgaag ccgacgccct cgaccaagtt ccgctgctcg cgcaacgggt
     2341 cgccgtagtg cgcggcaacg ccaaggtcgg tgggcttgag gtctggggtg gggcctggtg
     2401 tgggctcgct gtcagtcgcg acggcccctg gcagttggag caaggggctg cggtagccct
     2461 cgttctggtc gctcgcccgc gccgaatctg aaggcgcact catgcgagcc agtgtaggtc
     2521 agctctcgtg ggcgagcagg tcctcgctgt gcgggccagt tggatccgcg aggtcccaca
     2581 ggagcgagag gggcaacccc tcggccagca ggtgcatcgg ggcgtcctcg gtgagtgcca
     2641 tcagtgtcat cacgaactaa tgatcgacag gcgccggggg tcactgacca gccgatgggg
     2701 tgatagatcc tgcgcatctg ccatacgggc ccgaaatgcc cgctgaaatc gattgccgac
     2761 ccctcgccac gtgattactt tggacgtaca cgagagagga ggtggtccgc agaatgtgta
     2821 gtaaacggac gcgtgaggtg gctgtcagct agccgccgtc ctttgcggac gtgccctccg
     2881 tggggcgacg tcgccggctc tcaagtcttt gggggtcatg ggtggcgact gcgaaaccac
     2941 gacagctcat ccgacccccg ggccgccgga catcgtccag ccggtccgct tgttctggct
     3001 cgttaagagt cgcgacaatg cggaaagccc gggggtcgac ccatgttcga ggctcaatgc
     3061 aacggggcaa gccacgccgt aggaggcaac acatggcgga cgccgaagtg ctcgcgagcg
     3121 tgctcagcac caccgaagga atcattcggg gcgtgagacc cgaccagctg gacctgccga
     3181 ccccgtgccc tgactacacg gtcggaacgc tcgtcgccca catggtgacg tggagcaaca
     3241 cgttcgctga cggcgccgag cagctgccgc cgaataccga ccgtgccgag taccgggccg
     3301 acgaccgcgc aggtgacctg ttcagtgcgg cgtcggcgcg gaccgttcag gcgtttcagg
     3361 gcggtgcagc cgaccggatg atcgacctcg gcaacggccc gtcacccggg gagatgctgt
     3421 tcggcctgat gctcatggag tacatcgggc atggctggga cctcgccgtg gcaacggatc
     3481 agccgacccc gttttcggag gctgggatcg gagccgccct cgcgaccggg cggaatatgt
     3541 tgaagcccga gtaccgcggc ccggacaaga gcttcggcga cattgtcgag gtcggcgacg
     3601 aagcgccggc cctcgaccag ttgatcgggt tcctgggcag agacccagcg cgctagctgg
     3661 ccttggtgag acgcgccgac aggtgcggtc cgagcggttg accgaccgcg gccatgtcgt
     3721 aggcgtacag caggtcgccg tcgaccaacc cgtagagccg atggccggcg gtgacctctt
     3781 ttgccgtcgc ggtgcgggcc acgacgtcgg tgctcatctc gatgcgaccg aacgcgaggt
     3841 tgccgatcca gatctcggcg aagccactcg catgcgccaa cacgatctcg agaccacccg
     3901 cagcatcaac gcgccagtaa cccgactcgc cggcgcctgc gcgcaacacg ttgcccgcgt
     3961 cgtcgagtac ccagcttcgg ctcacgtagg tcagcaccgg cttgccggcg tggccgaaca
     4021 cgatctcctg cccgaacctg aaggactcga tcgtcgggta gccgccgacg ccgacaccgc
     4081 gccaggcccc taacaggaag gcgagcggct cgagcga
//
LOCUS       DASWVF010000280         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_211568, whole genome shotgun sequence.
ACCESSION   DASWVF010000280 DASWVF010000000
VERSION     DASWVF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211568"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..459)
                     /locus_tag="VGD55_04985"
     CDS             complement(61..459)
                     /locus_tag="VGD55_04985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY0869730.1"
                     /translation="MDTRLQVVVGAAIVDGERVLAAQRAEPPSLAGFWEFPGGKVDPG
                     ETDEAALIRECREELAIDIMLGARVGRDWPIGEHGILRVWLAAVTAGEPTAIEHCALR
                     WLTVDELHSVDWVPADLPIVDKLASMMQAR"
     gene            534..818
                     /locus_tag="VGD55_04990"
     CDS             534..818
                     /locus_tag="VGD55_04990"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0869731.1"
                     /translation="MEPLSAVEVARALAELKDWTGDTASLRRSIKASSFAAGIRLVDA
                     VAEIADAMDHHPDIDIRWTTSTCVCSTHSAGGVTELDVELARRIDDLAGS"
     gene            940..>1553
                     /locus_tag="VGD55_04995"
     CDS             940..>1553
                     /locus_tag="VGD55_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0869732.1"
                     /translation="MPPVIRVENLVKEFRRPRKQEGRFGTVRTLFTRQFDSVRAVDEV
                     NFAIEPGELVGYIGPNGAGKSTTIKMMTGILVPTSGTVEVGGLVPWRERERNAMQIGV
                     VFGQRSQLWWDLPLIESFNLIAKLYRVPDPKYKRNLDRFIALLDMEEFLERPVRQLSL
                     GQRMRGDLAASMLYEPQILYLDEPTIGLDVVAKENMRIFIEEMNR"
BASE COUNT          291 a          531 c          479 g          252 t
ORIGIN      
        1 ctggagccag cgtttcgacg acctgatcgg tgaataccag tcggtcagca agccgcgcgc
       61 ctagcgggcc tgcatcatcg aggcaagctt gtcgacgatc ggcagatccg caggtaccca
      121 gtcgacagag tgcagctcat cgacggtcag ccaccgcaac gcgcaatgct cgatcgccgt
      181 cggttcgcct gccgtaacag ccgccagcca cacccgcagg atgccatgct cgccaatcgg
      241 ccaatcgcga ccgacccgcg cgccgagcat gatgtcgatc gcgagctcct cgcggcactc
      301 gcggatgagt gcagcctcgt cggtctcgcc cgggtcaact tttcctcccg gaaactccca
      361 gaaaccggcg agcgacggcg gctcggcgcg ctgcgctgcg agcacccgct ccccgtcgac
      421 tatggcggcc ccgacgacca cttgcaggcg cgtgtccaca gtcgcgcagt ctgccacgat
      481 ctcgcccaga cgccgccttg actccgtgac ggttgaccgg cgacggtgga ttcatggaac
      541 cactgagtgc ggtcgaggtg gcccgcgcgc tcgccgagct gaaggactgg acgggcgaca
      601 ccgccagcct gcgtcgcagc atcaaggcat cgagcttcgc cgccggcatc cggctcgtcg
      661 atgccgtcgc cgagatcgcc gacgccatgg accatcaccc cgacatcgac atcagatgga
      721 cgacgagcac ctgcgtctgc tcgacgcact ccgcgggcgg cgtgaccgag ttggacgtcg
      781 aactggcgcg ccggatcgac gatctggccg gcagctagcc gcggtcacgc tgacgcatcc
      841 gtaacccggt cgccttcggc gcctagagtg cgataaacca gtacctgaag acctaaggtc
      901 tagtcctttt cgcatgcccg gaacacacca ggagatgtcg tgccgccggt gatccgcgtc
      961 gagaatctcg tgaaggagtt ccggcgccca cgcaagcagg aaggccggtt cggcactgtt
     1021 cgcacgctgt tcacccggca gttcgattcg gtccgtgccg tcgacgaggt gaacttcgcg
     1081 atcgagcctg gcgagctggt cggctacatc ggcccgaacg gcgcgggcaa gagcaccacc
     1141 atcaagatga tgaccggcat cctggtgccg accagcggca ccgtcgaggt cggtggtctc
     1201 gtgccgtggc gcgaacgcga gcgcaacgcg atgcagatcg gcgtcgtgtt cggccagcgc
     1261 agccagttgt ggtgggacct cccactcatc gagtcgttca acctcatcgc aaagctgtat
     1321 cgggtgcctg acccgaagta caaacgcaac ctcgatcgct tcatcgcgct gctcgacatg
     1381 gaggagttcc tcgaacgacc ggtgcgtcaa ctgagcctcg gccagcgaat gcgcggtgac
     1441 ctagcggcgt ccatgctcta cgaaccgcag attctgtacc tcgacgagcc gaccatcggc
     1501 ctcgacgtcg tggccaaaga gaacatgcgc atcttcatcg aagagatgaa ccg
//
LOCUS       DASWVF010000281         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_364221, whole genome shotgun sequence.
ACCESSION   DASWVF010000281 DASWVF010000000
VERSION     DASWVF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364221"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGD55_05000"
     CDS             complement(<1..653)
                     /locus_tag="VGD55_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869733.1"
                     /translation="MDHQLIRRLHQATGARSQAWSNGEQLVGSAPNADAYRVRSAAFA
                     DEVVAEHVQSVLTAGQEAPSPAQEEELVDAILARMFGAGRLQQILEDPALADMTDLDI
                     NGCEEVWATFTDGRKLRLPAVADTDDELVELVQTLASYVGRNPRPWDAASPQLNLRLS
                     DGSRLAATHPVLSGRPSVSVRRSLHPRVFLADLVRLGSVSPALATFLQAAVRARFNI"
     gene            complement(638..1468)
                     /locus_tag="VGD55_05005"
     CDS             complement(638..1468)
                     /locus_tag="VGD55_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869734.1"
                     /translation="MGFLTVVCSDKGSPGATTLAMLLAAAHPGRPVLVEADPAGGDVP
                     ARLFAGTGRPHAGTANLLTLATDSRRGSHPQMLSAHAAITAVGVTLVPGLSNADQQVG
                     LLPLWPSLAAVLAASECDVIADLGRICAVHPGLAAAAAAHRILLAVRPNLEQLLRVRD
                     RVRHLLALIGEDASRLVVVVICPEKTAERDQRAVARVLADAGLSAVRVVWIPFAPNEV
                     AAFYAGQLNRRGSLSRSATALAAELSFGVGAPAWEAAVRSTSGAHRGAIAGEPAWTTS
                     "
     gene            complement(1474..2166)
                     /locus_tag="VGD55_05010"
     CDS             complement(1474..2166)
                     /locus_tag="VGD55_05010"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY0869735.1"
                     /translation="MSTTTIGVSAPLQGVPRTRLHAVPGRGLPTRQRRPAMITAAVLL
                     IVAGALGGLQVLTASNAKVSVLVLVQPVPAGHVIGASDLGSTALSGKVAYTPASAEAS
                     VVGATAARDLFAGQLVTHSMIATAAVPDVTHALVGLQLSAGQVPSAGLADGDFVELVD
                     VPSATGVAALSASSPTSSSSVLATGTVYSIATSTGASGAALVTVLVPRAQAEALSVAA
                     SSSQVSLIRVGS"
     gene            complement(2189..>3186)
                     /locus_tag="VGD55_05015"
     CDS             complement(2189..>3186)
                     /locus_tag="VGD55_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869736.1"
                     /translation="RAVRRLMLMRIAGRRSSAACLCLSVVCAALIGVGIGNLASASAS
                     ASASTPVTCTNLDDPRCVIHVSDPGGGTGPTAPVSTGAGGCRPPGGPGIPAGVDCNGC
                     IWSAYSPAPGQVDDDPAYEEWLAAGGKGSVQQQDCYPGPTTSVQYFPPGAAPPPPGVP
                     AAQLGVDAASLLPFPTVTMGSAPGAEGRAFAMTFVNLPTFLWVDNAQWIPLAATANDG
                     VKAVTATAVPSYVTWVMGDGRSTICSGPGVAWQPGIEASGCSHTYTTSSVHEPQVGSD
                     VNDRPYTVTATVTYQVTWTCAGQCGGVASGTVGAVVGPTSRSSLVVGEIQTVVSG"
BASE COUNT          579 a         1174 c          993 g          440 t
ORIGIN      
        1 atgatgttga agcgcgcccg caccgcggcc tgcaggaacg tagcaagtgc ggggctgacg
       61 ctgcccagtc gcaccagatc ggccaagaac acgcgcggat gcagcgaccg gcgcaccgac
      121 accgaaggcc gcccggacaa caccgggtgc gtcgcggcca gccgtgaccc gtccgacaaa
      181 cgaaggttca gctgcgggct ggccgcgtcc catggccgcg gattgcgtcc gacataggac
      241 gccaatgttt gcaccaactc gacgagctcg tcgtcggtgt cggccacggc aggcagccga
      301 agtttgcggc cgtcggtgaa cgtcgcccac acctcctcgc agccgttgat gtccaggtcg
      361 gtcatgtccg cgagtgccgg gtcttcgagg atctgctgca accggccggc gccgaacatc
      421 cgcgccagga tcgcgtcaac gagttcctcc tcctgcgcgg gcgacggtgc ttcctggcct
      481 gccgtgagca cgctctgcac gtgctccgcg acgacctcgt cggcgaacgc cgccgaccgc
      541 acccggtacg cgtccgcgtt gggcgcgctg cccactaact gttcgccgtt cgaccaggct
      601 tgggagcgcg cgccggtggc ctggtggagg cggcggatca gctggtggtc cacgccggct
      661 cccccgcaat ggcgccccgg tgcgcaccgg acgttgagcg caccgcagct tcccaagctg
      721 gtgcaccgac gccgaacgaa agctcggcag ccaacgcagt cgcgctgcgc gacaaggagc
      781 cccgccggtt cagctggccg gcgtagaacg ccgcaacttc atttggggca aagggaatcc
      841 agacgacgcg caccgcggaa agaccggcgt cggctaggac ccgcgcgacc gcgcgctgat
      901 cccgctcggc cgttttctct gggcagatga cgacaaccac aaggcgggac gcgtcctcgc
      961 caatgagtgc gagcaggtga cggacccggt ctcgtacacg cagcagttgt tccaggttgg
     1021 ggcgaaccgc gagcaggatg cggtgcgccg cggccgccgc cgccaagccc ggatgcaccg
     1081 cgcagatgcg acccaagtcc gcgatcacat cgcactcgct cgcagccaat accgccgcga
     1141 ggctcggcca cagcggaaga agcccaacct gctgatcggc gttgctcaat cccggcacca
     1201 gcgtcacacc gactgccgtg atcgctgcgt gcgcgctgag catctgcggg tgcgacccgc
     1261 ggcgtgagtc ggtggcgagg gtgagcaggt tcgccgtgcc cgcgtgcggc cggccggtgc
     1321 ccgcgaacag tcgagccggc acgtcgccgc cggccgggtc agcctcgaca agaaccgggc
     1381 gacccggatg cgccgccgct aacaacatgg ccagcgtcgt cgcgcccggg ctgcccttgt
     1441 ccgagcacac caccgtcagg aaacccacgc cggtcagctc cccaccctga tcaacgaaac
     1501 ctgactgctg ctcgccgcga ccgacaaagc ctcggcctgg gcacgtggca ccagcacggt
     1561 caccagcgcc gcaccggacg cgcccgttga cgtggcgatc gaatacaccg tcccggtggc
     1621 cagcacgctc gacgacgacg ttgggctcga cgccgacaac gcagcgacac cggtagcgga
     1681 cggcacgtcc accaactcca caaagtcgcc gtcggccaga cccgcagatg gcacctgtcc
     1741 cgcggacagc tgcaacccga cgagcgcatg cgtcacgtcg ggaaccgctg ccgtcgcgat
     1801 catcgaatgc gtcaccagct gcccagcgaa cagatcgcgg gccgcggtcg caccgaccac
     1861 gctggcctcc gctgaagcag gcgtgtacgc gaccttcccc gacagcgcgg tgctccccag
     1921 atcgctcgca ccgatgacat gacccgcagg caccggctgc accaacacca gcaccgacac
     1981 cttcgcgttc gacgcggtca acacctgcaa gccaccgagt gcgcccgcaa cgatgagcag
     2041 taccgcggcc gtgatcatgg ccgggcgtcg ttgccgcgtc ggcaacccgc gaccaggtac
     2101 tgcgtgcaaa cgcgtgcgag gcacaccctg taacggagca gaaactccga tcgtcgtcgt
     2161 actcattgcg gtccccccgt tgcgaacatc agcccgatac caccgtttgg atctccccga
     2221 cgaccagcga gctgcgtgac gtcgggccga ctaccgcacc aacagtgccg ctcgccacac
     2281 ccccgcattg gcccgcgcac gtccacgtca cctgatacgt cacggtcgcc gtcacggtgt
     2341 acgggcggtc gttgacgtca ctgccaacct gcggctcgtg caccgacgac gttgtgtagg
     2401 tgtgggaaca gcccgaggcc tcgataccgg gttgccacgc cacccctggc ccgctgcaga
     2461 tcgtgctcct gccatccccc atcacccacg tgacatagga cggaaccgca gtcgccgtca
     2521 ccgctttcac tccatcgtta gcggtcgctg ccagcggaat ccattgcgcg ttgtcgaccc
     2581 agagaaacgt cggcagattc acgaacgtca tcgcgaacgc ccgcccctcc gcgcccggtg
     2641 ccgaacccat cgtcaccgtc gggaaaggca gcagcgacgc cgcatcgaca cccaactgcg
     2701 cggccggaac gccgggcggt ggcggcgcag cacccggggg gaaatattga accgatgtcg
     2761 tgggtccggg atagcagtcc tgttgttgga cgctgccttt cccgccggca gcaagccatt
     2821 cctcgtaggc cggatcatcg tcgacctgac ccggcgcggg cgaatacgcg ctccagatgc
     2881 aaccgttgca gtccacaccg gccggaatcc ctggaccacc aggcggccta cagccgccag
     2941 cacccgtgct gaccggagca gtcggtccag ttccgccgcc cgggtcactc acatgaatga
     3001 cacagcgcgg atcgtccaag ttcgtgcatg tgaccggcgt gcttgccgat gccgatgccg
     3061 atgccgatgc cagattgccg atcccaacgc cgatcagcgc cgcacacacg acagaaagac
     3121 aaagacacgc cgcactgctt cgccgcccgg cgatccgcat caacatgaga cgacggacgg
     3181 cgcgag
//
LOCUS       DASWVF010000282         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_262667, whole genome shotgun sequence.
ACCESSION   DASWVF010000282 DASWVF010000000
VERSION     DASWVF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262667"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGD55_05020"
     CDS             <1..989
                     /locus_tag="VGD55_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0869737.1"
                     /translation="DTIRGDVAEATWRLVDVRDPVLVERLAGVDVIVHLAVDSTVDHD
                     DAAHHDVGAKATATVLTAAPAAGVKHVVLVTSAMVYGAHADNAVPLSEDAELRATADS
                     GWLGDLLEIERLAAQAPRTHPGLLVTVLRPAALVGGGVDTIFTRHFEAPRLLAVRGSR
                     TRWQFCHVDDLLSAVELVVTGGLGALAPDESGSLGYVVAAVGSDGWLEQGDVERLTGK
                     RHIELPASVAFGTAERLHRLGVTPAGSNDLQFLVHPWVVQATRLPAAGWSPAYGNEAA
                     LGVAIAESSGRLAIGGHRLGRRNAAAGAAVGATVALVGTAALVRRARRRRHG"
     gene            982..1428
                     /locus_tag="VGD55_05025"
     CDS             982..1428
                     /locus_tag="VGD55_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY0869738.1"
                     /translation="MADVVRLLGIRTEPLSVDEVLAAVADPSAGGTAVFVGTVRSVDE
                     ERSVESLHYEAHPDALSVLREVAEDIAAKYSPIAVAAVHRVGLLVVGDLAVVVAAAAG
                     HRNEAFVAARELIDEVKARVPIWKRQSFTDGAVAWVGIETSLDGGE"
     gene            1455..1700
                     /locus_tag="VGD55_05030"
     CDS             1455..1700
                     /locus_tag="VGD55_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869739.1"
                     /translation="MKVLLWLMIPVAALIVGIIWASLRSRPPRPAAMQDSMASFSRFR
                     HALASNPASSSRRQPEQVSSAVARGAHGSTGIDDPPG"
     gene            1867..2382
                     /locus_tag="VGD55_05035"
     CDS             1867..2382
                     /locus_tag="VGD55_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869740.1"
                     /translation="MEHVVFFPAPDGTPAFRRFATLDDAARFVEHLRNVENVSGASVH
                     ALTEVPLAFKAWYRVELPDAVSEPLATEASSEEPSAPTTIAEVLESYAATDVLPNNGS
                     VELPEAMGSVTGIMVPEREGSSPAPIALSQAADQSLFSQPVPEPERAEAQVLEPAFGT
                     RRERGTGYFVR"
     gene            2504..3547
                     /locus_tag="VGD55_05040"
     CDS             2504..3547
                     /locus_tag="VGD55_05040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY0869741.1"
                     /translation="MRRQAMTLLVCGLLALGLAVVAARLPVPYVRDVPGPVTDTLGSA
                     HGKALITIGHPTATPQGHIFLVTVSEYGGPRKNISGLDVLIGWWNRSDAVIPRRLLYS
                     DTQTPQQVATQDNNDMIESQEEAKVAALRYLGYPLKPGVDVAEVVLPALKGKLMPDDI
                     IVGVDGTAVPNRDALLKITQAHKAGDVITLNVVRGTAAIDVPVTLQPKAVGGTGPTIG
                     ISVVDSFTKPFTIDINLADVGGPSAGTAFALGIIDKLTDGNLTGGHTVAVTGTIDADG
                     NVGAIGGPIQKMAGARHAGATAFLLPKDNCAEALKAVPHGLRVVPITTLAGAVDVLKE
                     IRAGDVNLPSCPS"
     gene            complement(3554..4087)
                     /locus_tag="VGD55_05045"
     CDS             complement(3554..4087)
                     /locus_tag="VGD55_05045"
                     /inference="COORDINATES: protein motif:HMM:NF040618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HEY0869742.1"
                     /translation="METPPTRHALLGAIAEVDRHLAEGGWDQPTRLYALVRTADLLAS
                     EPILSDTLGLAATPDADSLTPVEQEAPPAGTPLDEWLGEIGWPSEVAGCALSQEVLTL
                     PPSVEADLPPDADAVAWAASHPLRREVRMLVGVLRDGSRTATLRVRGAADNPDDVLIG
                     DDLVPRLADALAATLLD"
BASE COUNT          703 a         1407 c         1414 g          702 t
ORIGIN      
        1 tcgacacgat caggggcgac gttgcggaag cgacgtggcg tctcgtcgac gtccgcgacc
       61 ccgtgcttgt cgagcgactc gcgggcgtcg acgtgatcgt gcacctcgca gtcgacagca
      121 ccgtcgacca cgatgacgcg gcacatcacg acgtgggtgc caaagcgacg gcgacggtgc
      181 tgacggcggc gcccgctgcg ggagtcaaac acgtcgtgct cgtgacgagc gcgatggtct
      241 acggcgcgca cgccgacaat gcggtgccgt tgtcggagga cgctgagttg cgggcaaccg
      301 ccgacagcgg atggcttggc gacctccttg agatcgagcg gctggcggca caggcgccgc
      361 gcacccaccc cggccttctg gtgaccgtcc tgcgtcccgc cgcgctcgtc ggcgggggtg
      421 tcgacacgat cttcacgcgg catttcgagg ctccccggtt gctcgcagtc aggggatcgc
      481 gtacccgctg gcagttctgt cacgtcgacg atctgctgtc agcggtcgag ttagtggtga
      541 ccggtggatt gggcgcgctc gcccccgatg agagcggcag cctggggtac gttgtggccg
      601 ctgtcggcag tgacggctgg cttgagcaag gcgatgtcga gcgactgaca ggcaagcgac
      661 acatcgagtt gcccgcgtcg gttgccttcg gtacggccga gcggctgcac cgcctcgggg
      721 tcaccccagc cggctcgaat gatcttcagt tcctcgtgca tccgtgggtc gtacaggcga
      781 cccgcctgcc tgcggccggc tggagccccg cctatggcaa cgaggcggcg ctcggcgtcg
      841 ccatcgcgga atcctcgggt cggctcgcga tcggcggcca tcgactcgga cgccgcaacg
      901 ccgccgccgg cgcagctgta ggcgcgaccg tcgccctggt cggcactgcc gcgctggtcc
      961 gccgggcaag gcgtcgccga catggctgac gtcgtccgtc tcctgggtat tcgcaccgaa
     1021 ccgctgtcag tcgacgaagt tctcgcggca gttgctgacc cctcagcagg ggggacggcg
     1081 gtcttcgtcg gcaccgtacg gtcggtcgac gaggagcgat ccgtcgaatc gctgcactat
     1141 gaggcgcacc ccgacgcgtt gagcgtgctg cgcgaggtcg ccgaagacat cgcggcgaag
     1201 tactcaccca tagcagtggc ggcagtccac cgcgtcggat tgctcgtggt gggcgatctg
     1261 gcagtcgtgg tcgctgcggc cgctggccac cgcaacgagg ccttcgtcgc cgcacgcgaa
     1321 ctgatcgacg aggtgaaggc gcgagtgccg atatggaaac gccaaagctt caccgacggt
     1381 gcggtcgcct gggtggggat cgagactagt ctggacggcg gggagtaacg ggaaaggatt
     1441 gtcgcgagct accgatgaag gtcttgctgt ggttgatgat tccggtcgca gcgctgatcg
     1501 tggggatcat ctgggcgtct cttcggagcc ggccccccag gccggcggcg atgcaggatt
     1561 cgatggcgtc gttctcacgc ttccgtcatg cgctggcaag caacccggcg agttcgagca
     1621 ggcggcaacc cgaacaggtg agctcagcag tggcgcgcgg cgcccacggc tcgacgggta
     1681 tagacgaccc gcccggatag cccccgagtg gcccttggaa tgggcttgat gtaacccgtt
     1741 tgccgacaga cacgtgcgct agacggctca aggtgacgta gtgcacatgc cgatctacgg
     1801 aatgtcaacg tccctaggag ccctgtcgcc aaaggcgatc cgggccagcc gcctgagtga
     1861 ggcatcgtgg aacacgtcgt cttcttcccc gcgccggacg gcaccccggc tttccgtcgc
     1921 ttcgccaccc tcgacgatgc ggctcgattc gtcgagcacc tgcgcaacgt cgagaacgtc
     1981 agtggcgcct ccgtacatgc tctgaccgag gtaccgctgg ccttcaaggc gtggtaccgc
     2041 gtggaacttc ctgacgcagt gtcggagcca ctggccaccg aagcatcgtc ggaggagccc
     2101 agcgcaccca cgacgatcgc cgaggttctc gagagctacg cggcaaccga cgtgctgccc
     2161 aacaacgggt ccgtcgagct tcccgaggcc atgggttcag tcaccggcat catggtcccc
     2221 gagcgcgaag gttcgtctcc ggccccgatc gctttgagcc aggccgccga ccagtccctg
     2281 ttctcccagc ctgttcctga gccagagcgg gccgaagctc aagtgctaga gccagcgttc
     2341 ggtactcgcc gcgagcgcgg caccgggtac ttcgttcgct aacagtcgag cgagccctac
     2401 cgccgctccc tcaacccccc aaagctcacc ccccgagctg catacgagcc gtgacccttc
     2461 agggtcacgg ctcgcgtgtt tgcgtgccaa ctacgctggc cgcatgcgac gtcaggcaat
     2521 gaccctgctg gtgtgtggcc tgctggcgct tgggctcgca gtcgtcgcgg cgcggctgcc
     2581 cgtgccgtac gtacgcgacg taccgggacc ggtgaccgac acgctgggct cagcacacgg
     2641 caaggcgctc attacgatcg ggcacccgac ggcgaccccg caaggccata tcttcctggt
     2701 caccgtgagc gagtacggcg gaccgcgtaa gaacatctcc gggctcgatg tcttgatcgg
     2761 gtggtggaac cgcagcgacg cggtcattcc gcggcggttg ctgtattcag acacccagac
     2821 accacaacag gtcgccacgc aggacaacaa cgacatgatc gagtcgcagg aagaagcgaa
     2881 ggtcgccgcg ctgcgctacc tgggctatcc gttgaagccc ggtgtcgatg tcgccgaggt
     2941 ggtgttgccc gcgttgaagg gcaagctgat gcccgacgac atcatcgtgg gtgttgatgg
     3001 caccgcagtc ccgaaccgtg acgcgcttct gaaaatcact caggcacaca aggcgggcga
     3061 cgtcatcacg ttgaacgtcg tacgagggac cgcggcgatc gacgtccctg tcacgcttca
     3121 gccaaaggcc gtcggcggaa ctggtccgac gatcggcatc agtgtggtcg acagcttcac
     3181 caaaccgttc acgatcgaca tcaacctcgc tgacgttggt ggcccgagcg caggtaccgc
     3241 attcgcactc ggcatcatcg acaagctcac cgatggcaac ctgaccggcg gtcacaccgt
     3301 tgcggtcacc ggaaccatcg acgctgatgg caatgtcggt gcgatcggcg gaccgattca
     3361 aaagatggca ggcgcccggc acgcaggggc tacggcgttc ctgctcccca aagacaactg
     3421 cgccgaggcg ctcaaggctg ttccgcacgg attgcgcgtt gtgccgatca ccaccctcgc
     3481 cggtgctgtc gacgtcctga aagagattcg agccggcgac gtgaacctgc cgagctgtcc
     3541 gagctgatcg cggtcagtcg agcagtgttg ccgcgagcgc gtcggcaagg cgaggcacta
     3601 gatcgtcgcc aatgaggacg tcgtcggggt tgtccgccgc gccgcgcact cgcagcgtcg
     3661 cggtacgtga gccgtcgcgc aagacgccca cgagcatccg cacttcacgg cgcaacgggt
     3721 ggctggcagc ccaggcgacg gcatcggcgt ccggcggaag gtcagcttcg accgaaggcg
     3781 gcagcgtcag aacctcctgc gagagcgcgc acccagccac ctccgacggc cagccgatct
     3841 cgcctagcca ttcatcgagc ggcgtgcccg ctggcggcgc ttcctgttcg accggcgtga
     3901 gcgaatcggc gtccggggtg gcggcaagcc cgagcgtgtc ggacagaatc ggttcggagg
     3961 ccaacagatc ggcagttcgc actagtgcgt acagccgggt cggctggtcc caacctccct
     4021 cggcaagatg cctgtcgact tcggcgatcg ctccgagcaa ggcgtgccgg gttggcggcg
     4081 tctccatggc cgccatcttg cccgaccgta ggtccccgcg actccgggag cggttcggta
     4141 ggttgaggcc gtgacctttc cccgccccga tcgccggccg tacggctcgg gtgacggccg
     4201 gtcccgccca ccgctcccgc gaccgg
//
LOCUS       DASWVF010000283         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_135255, whole genome shotgun sequence.
ACCESSION   DASWVF010000283 DASWVF010000000
VERSION     DASWVF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135255"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VGD55_05050"
     CDS             complement(<1..1236)
                     /locus_tag="VGD55_05050"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0869743.1"
                     /translation="MKAGVHPLSAAVVGACLVLTGGLAAVANVVNAHSNQRLLVQQVQ
                     QAAVALSIALPVVQSQLVDAAQVAIDTNGDPAVFKRFVSAGIARTGSQSAISLWQIKD
                     GQARQIVSLGGGSTLVSQHLDSAFFASVKPSTQLYLTKMLPGATPHLGFAEMPTGDTA
                     GYVVYQEAPLSPTKRVTIPATSPFADLKVALYLGPLTTNDQLLEATTATPIQGHTAQT
                     SLPLGNTEVTIVGASMKPLAGGLSNALMWIVIGSGIALALTIGWIVEYAIRRRLHAED
                     LARDNDRLYREQRDIAGTLQHALLPQLPEVAGLDVAARYVAGVAGLEVGGDWYDVMQT
                     DDDHCVFFVGDVSGSGLNAAALMGSLRYAARAYIAQGDDIQTVLVKLSELHDIERDQH
                     FATVLAGDIDVAGRRVTLVS"
     gene            complement(1264..>2525)
                     /locus_tag="VGD55_05055"
     CDS             complement(1264..>2525)
                     /locus_tag="VGD55_05055"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY0869744.1"
                     /translation="QRPRAVVDAAPSAILCVDLDGFRDVNEVVGYAVGDGVLVEFAGR
                     LKACLRARDMLVRIGADEFGIYIRAADERFVHQIAERVMVEAERPFHIAGNVVHLGAC
                     IGIAMSTDVAPDESLSMMDGLLRHATAALRLAKREGRGRIRRLIDPGGEEVDGPTATA
                     AIARRIRPALLAGDFSLHYQQLLDIPSMECHEVEALMRWTDPELGAVGPNRFIPIAEH
                     SAQIVALGRWALHEACHQAHEWLDAGNPRTVGVNVSAYQLTDPHFVDDVDEALDLTGI
                     PAELLRLEITESAAVSDMCGTVGRLNQLRTLGVNLSLDDFGTGYSSLAMLRQLPITTL
                     KIDRSFVDRLDGPNNYPDAVLIEAVIATAHAYGLTVVAEGIERPAQLALLRNMGCDTA
                     QGFLLHRPQPANEIRWAPSTISLTTAK"
BASE COUNT          448 a          794 c          815 g          468 t
ORIGIN      
        1 ggagacgagc gtcacccgac gtccggccac atcgatgtca ccggccagca ccgtcgcgaa
       61 gtgctggtct cgctcgatgt cgtggagctc gcttaacttg accaggacgg tctggatgtc
      121 gtcgccttgc gcgatgtagg cgcgcgcggc gtagcgcaac gaccccatca gcgccgctgc
      181 gttgagaccg cttcccgaaa cgtcgccgac gaagaagacg caatggtcgt cgtccgtttg
      241 catcacgtcg taccagtcgc caccgacctc gaggccagcg acacccgcga cgtagcgcgc
      301 cgcgacgtcg agccctgcga cctcgggcaa ttgcggcaac agcgcgtgct gcagcgtgcc
      361 ggcgatatcg cgttgctcgc ggtagagccg gtcgttgtca cgggccagat cttcggcgtg
      421 cagccgacgg cgaattgcat actcgacgat ccagccgatc gtcagcgcca acgcgatacc
      481 cgacccgatc acgatccaca tcagtgcgtt cgagaggccg ccggcgagcg gcttcatcga
      541 cgccccgact atcgtcactt ccgtgttgcc gagtggcagc gacgtctgcg cggtgtgtcc
      601 ttggatcggg gtcgcagtgg tggcctcgag caattggtcg ttcgtcgtca gcggcccgag
      661 atagagcgcg accttgaggt cggcgaatgg cgacgtcgca gggatcgtca cgcgcttcgt
      721 tggtgacagc ggagcttcct ggtagacgac gtagccggcc gtgtcaccgg ttggcatctc
      781 cgcgaacccc agatgtggtg tcgcaccggg cagcattttt gtgaggtaca gctgggtgct
      841 tggcttgacg ctcgcgaaaa atgccgagtc gagatgctgc gagacgaggg ttgagccacc
      901 acccaagctc acgatctgac gggcctggcc atccttgatc tgccacaacg agattgcgga
      961 ttgcgagcca gttcgcgcaa tgcctgcaga gacgaagcgc ttgaacaccg ccgggtcgcc
     1021 gttcgtatcg atcgccacct gcgccgcatc aacaagctgc gactggacga cgggaagtgc
     1081 gatcgacaac gcgactgcgg cttgttgaac ctgctgcacg agcaagcgct gattgctgtg
     1141 cgcattcacg acgttggcca ccgccgccag gccgccggtc aagacgaggc aggctccgac
     1201 gactgccgca ctgagcgggt gtacgcctgc cttcatctcg gtaactcccg gagcgttgtc
     1261 agctcacttt gctgtcgtga ggctgatcgt cgatggcgcc catctgatct cgttagccgg
     1321 ttgtggtctg tgcagaagaa atccttgggc cgtgtcgcag cccatgttgc ggagcagcgc
     1381 tagttgggcc gggcgctcga tgccttcggc gaccacggtg agcccgtagg cgtgcgcagt
     1441 cgcgatgacg gcctcgatga gcaccgcgtc ggggtagttg ttcggaccat caagccgatc
     1501 gacaaaggat cggtcgatct ttagcgttgt gatcggaagt tgccgaagca tcgcgagtga
     1561 tgagtaaccg gtaccaaagt catcaagcga cagattgacg ccgagtgttc tcaactggtt
     1621 caggcgtccg acggtgccgc acatgtcgga gaccgcagcc gactcggtga tttcgagccg
     1681 gagcagttcg gcagggattc cggtcaggtc gagcgcctca tcgacgtcgt cgacaaaatg
     1741 cggatcggtg agttgatacg ccgacacgtt cacgccgacg gttcttgggt tgcccgcgtc
     1801 cagccactcg tgcgcctgat ggcacgcctc gtgcaacgcc caccggccaa gcgccacgat
     1861 ctgtgcgctg tgttcggcga tggggatgaa cctgttcggc ccaaccgcgc cgagttcggg
     1921 atcggtccag cgcatgaggg cttcgacttc gtggcactcc atgctcggga tgtcgagaag
     1981 ctgctggtag tgcaagctga agtcgcctgc aagcaacgcc ggccggattc tgcgggcgat
     2041 cgccgctgtg gcagtgggac cgtcgacttc ctcgccgcca gggtcaatga gccggcggat
     2101 tcggccgcga ccttcacgct tggccagccg cagtgccgcc gtggcatgcc gaaggagacc
     2161 gtccatcatg ctcaggctct cgtcgggagc cacgtcggtg ctcatcgcga tcccgatgca
     2221 agccccgaga tggacgacgt ttcccgcgat atggaacggc cgctcagctt ccaccatcac
     2281 gcgttcggca atctggtgca cgaagcgctc gtcggcggcc cttatataga tgccgaactc
     2341 atcagcgccg atgcgcacga gcatgtcgcg agctctcagg caggctttga ggcggccggc
     2401 gaattcgacc aggacgccgt cgccgactgc gtagccgacg acttcgttga cgtcacggaa
     2461 cccgtcaagg tcgacgcaca agatcgcgct cggcgcagca tcgacaaccg ccctcggtcg
     2521 ctgtc
//
LOCUS       DASWVF010000284         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_186151, whole genome shotgun sequence.
ACCESSION   DASWVF010000284 DASWVF010000000
VERSION     DASWVF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186151"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..387)
                     /locus_tag="VGD55_05060"
     CDS             complement(127..387)
                     /locus_tag="VGD55_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869745.1"
                     /translation="MHPDLLESLVRDRITELRGGRTHRSAPAGPEGSRGSRGSRRSRR
                     WASTGRAHVGRLFVRLGTSLIGEGAGHPVRYQALPEAACSTR"
     gene            complement(610..996)
                     /locus_tag="VGD55_05065"
     CDS             complement(610..996)
                     /locus_tag="VGD55_05065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0869746.1"
                     /translation="MRWGDMDAFGHVNNVDFLTYMQEARTGMLFAGEAAQRIPDLIKG
                     VVVARHEIDYRRPLEWRPEPIEVDVWTAEVKSSSFVLRYEIRDQLAGVVLAEALTVLV
                     PFDLAAMRPRRVSVDEREFLSRYLEP"
     gene            complement(1027..>2178)
                     /gene="ettA"
                     /locus_tag="VGD55_05070"
     CDS             complement(1027..>2178)
                     /gene="ettA"
                     /locus_tag="VGD55_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0869747.1"
                     /translation="KLLLQQPDLLLLDEPTNHLDAESVLWLEQHLEKYPGTVVAVTHD
                     RYFLENVAQWILEIDRGRTYPYEGNYTTYLEAKAARIKVEGAKDQKLRRRLEDELEWV
                     RSNPKARQAKSKARLDRYEEMAIEADKYKKLDFDEIQIPPGPRLGNIVVETSGLTKGF
                     DDRLLMENLSFSLPRNGIVGVIGPNGTGKTTLFKMLIGEEQPDAGTIKIGETVSISYV
                     DQSRSGLDPTKNLWEVVSDGLDYIKVGHVEMPSRAYVSAFGFKGPDQQKPTGVLSGGE
                     RNRLNLALTLKQGGNLLLLDEPTNDLDVETLASLENALLEFPGCAVITSHDRWFLDRV
                     ATHILAYEGDSNWFWFEGNFESYERNKVDRLGADAARPHRATYRKLTRG"
BASE COUNT          361 a          706 c          685 g          426 t
ORIGIN      
        1 cggacgacgg cagcggcagc cggggctcgc cgcttggcag gtgccgctgt cgcgggtgcc
       61 tttgtggcca ccgccttctt ggcgggcgtc gcctttttgg cggcgtcgcc tttttggcgg
      121 gcgcgctcat cgggtgctgc acgccgcctc tggcagcgcc tgataccgca ccggatggcc
      181 cgcgccctca ccgatcaacg acgtcccgag gcgcacgaac aacctaccca cgtgtgccct
      241 gccggtgctg gcccaacgcc gtgaacggcg cgaaccccgc gagccgcgcg aaccttcagg
      301 cccggccggg gccgaccggt gggttcgccc accacgcaac tcggtgatcc ggtcgcgtac
      361 caacgactca agcaggtctg gatgcatgtc tggctccttc acgagaactc ttgtgacaag
      421 tattcctgtg acaaggaatc ttgtcaagct cctgacaagg agtcttgtca agtcagcgcg
      481 agaggatcgc gcggcacatg cctgacccgc cacccgcacg gtcagcccct gcaaacgtca
      541 agttgccgcg cgggttgctc atctcgatcg aggccatcgc actgctgcca acctgaccat
      601 ggcgaaggct tacggttcga ggtaccggct cagaaactcg cgttcatcga ccgacacccg
      661 tcgcgggcgc atggctgcca gatcgaacgg caccagcacg gtcaacgcct ctgcgagcac
      721 gactccggcg agttggtcgc ggatctcgta gcgaagcacg aacgacgacg acttgacctc
      781 ggcggtccaa acgtcgacct cgatcggctc cgggcgccac tcgagcggac gccggtagtc
      841 gatctcgtgt cgcgccacca cgaccccctt gatgagatcg gggatccgct gcgcggcctc
      901 gccggcgaac agcatgccgg ttcgcgcttc ttgcatgtag gtaaggaaat ccacgttgtt
      961 cacgtgcccg aaggcgtcca tgtcgcccca gcgcacagag caacggaaag taaatcgcac
     1021 gcgctactag ccgcgggtga gcttgcggta ggtcgcgcga tgcggccggg cagcgtcggc
     1081 tccgaggcgg tcgaccttgt tcctctcgta cgactcgaag ttgccctcga accagaacca
     1141 gttcgagtca ccctcgtagg cgaggatgtg cgtcgcgacc cggtcgagga accaccggtc
     1201 gtggctcgtg ataaccgcac aaccggggaa ctcgagcaac gcgttctcaa gcgacgcgag
     1261 cgtctcgacg tcgaggtcgt tcgtcggctc gtcgagcagc agcaggttgc cgccctgctt
     1321 cagcgtcagc gccaggttca gccggttgcg ctcgccgccg gacaggacgc cggttggttt
     1381 ctgctggtct gggcctttga agccgaaggc cgatacatag gcccggctcg gcatctcgac
     1441 atggccgacc ttgatgtagt cgagcccgtc ggacacgacc tcccagaggt tcttggtcgg
     1501 gtcgaggccc gaccggctct ggtcgacgta ggaaatgctg actgtctcac cgatcttgat
     1561 ggtgccggcg tctggctgct cttcaccgat cagcatcttg aacagcgtcg tcttgccggt
     1621 gccgttaggc ccgatgacgc cgacaattcc gttacgtggc agcgaaaacg acaggttctc
     1681 catgagtaac ctgtcgtcga atcctttggt aagccctgac gtctcgacga cgatgttgcc
     1741 aagtcgaggg cccggcggga tctggatctc gtcgaagtcg agtttcttgt acttgtcggc
     1801 ttcgatggcc atctcttcgt agcggtcgag cctcgccttg ctctttgcct ggcgtgcctt
     1861 cggattcgac cgcacccact cgagttcgtc ttcgaggcgc ctgcgcagct tctgatcctt
     1921 ggcaccctcg accttgatgc gggctgcctt cgcctcgagg taggtcgtgt agttgccctc
     1981 gtaggggtag gtgcggccac ggtcgatctc gaggatccat tgcgcgacat tctcgaggaa
     2041 gtacctgtcg tgggtgaccg cgacgacggt gcccgggtac ttctctagat gctgttcgag
     2101 ccagagcacg ctctcggcgt cgaggtggtt ggtcggctcg tcgagcagca gcagatcggg
     2161 ctgctggagc aacaactt
//
LOCUS       DASWVF010000285         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_7868, whole genome shotgun sequence.
ACCESSION   DASWVF010000285 DASWVF010000000
VERSION     DASWVF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7868"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="sufB"
                     /locus_tag="VGD55_05075"
     CDS             complement(<1..876)
                     /gene="sufB"
                     /locus_tag="VGD55_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0869748.1"
                     /translation="MTTIEHPELDQLGRYKFGWADSDVAGAAARRGLNEDVVRDISAK
                     KSEPAWMLEQRLKGLRIFEKKPMPLWGADLSGIDFDNIKYFVRSTEKQADSWDDLPAD
                     IRNTYDKLGIPEAEKQRLISGVAAQYESEVVYHKIREDLEAQGVLFLDTDTGLREHED
                     LFREYFGSVIPAGDNKFSALNTAVWSGGSFIYVPPGIHVDIPLQAYFRINTENMGQFE
                     RTLIIVDEGSYIHYVEGCTAPVYSSDSLHSAVVEIIVKKNARCRYTTIQNWSNNVYNL
                     VTKRAVAQAGATMEWI"
     gene            complement(873..>1670)
                     /locus_tag="VGD55_05080"
     CDS             complement(873..>1670)
                     /locus_tag="VGD55_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0869749.1"
                     /translation="GANPTGDGGRARGGVMRARGEIGNIAVVKSTLAADASPGAHLGP
                     DDARTRDRVARALHEHGPQSAGALSASFGLTPAAVRRHLDALIAAGRVSEVPERKVHG
                     QLRGRGRPAKLFALTDDGREMFPHAYDDIAVDALNYLRGAGGDAAVSAFARQRLQGLE
                     TRYAAALADVPADRRPAELAAALTADGYAASAHEAGSGAQVCQHHCPIAHVAVDFPQL
                     CEAETDMFVRLLGTHVQRLATIAHGDGICTTHIPLVHVQTASGKVTS"
BASE COUNT          271 a          538 c          554 g          307 t
ORIGIN      
        1 gatccactcc atggtcgcgc cggcttgagc aaccgcgcgc ttggtgacca ggttgtagac
       61 gttgttcgac cagttctgga tcgtcgtgta acggcagcgc gcattcttct tcacgatgat
      121 ctcgacgacg gcgctgtgaa gggagtcact cgagtagacc ggcgctgtgc aaccttcgac
      181 gtagtggatg taggaaccct cgtcgacgat gatcagcgtg cgctcaaact gccccatgtt
      241 ctcggtgttg atgcggaagt aggcctggag cgggatgtcg acgtgaatac ccggcggcac
      301 atagatgaac gagccgcctg accagaccgc ggtgttgagc gccgagaact tgttgtcgcc
      361 agccggaatc accgagccga agtactcgcg gaacagatcc tcgtgctcgc gcaggccggt
      421 gtcggtgtcg aggaacagca cgccctgggc ctcaaggtct tcacgaatct tgtggtagac
      481 gacttctgac tcatactgcg cggccacgcc cgagatgagg cgctgcttct ccgcctcggg
      541 gatgccgagc ttgtcgtagg tgttgcggat gtcggcgggc aggtcgtccc agctgtcggc
      601 ctgcttctcg gtcgagcgca caaagtactt gatgttgtcg aagtcgatgc ccgacaggtc
      661 ggcgccccac agcggcatcg gcttcttctc gaagatgcgc agacccttga ggcgctgctc
      721 gagcatccaa gccggctcgc tcttcttcgc ggagatgtca cgtacgacgt cctcgttcag
      781 gccacgcctc gccgccgcgc ccgcgacgtc agagtcggcc caaccgaact tgtaccggcc
      841 gagctggtcg agctctgggt gctcgatcgt cgtcatgagg tgacctttcc ggacgcggtt
      901 tgtacgtgga ctaacggaat gtgggtcgtg catatgccgt cgccgtgggc gatggtggcg
      961 agccgctgca cgtgggtgcc gagcagtcgg acgaacatgt cggtttctgc ctcgcacaac
     1021 tgcgggaagt cgacggcgac gtgggcgatc gggcagtggt gctggcagac ctgggcgccg
     1081 ctgcccgcct cgtgcgcgga ggctgcgtag ccatcggccg tgagcgctgc agccaattcg
     1141 gccggacggc gatcagctgg cacatcagcg agcgcggccg cgtagcgggt ttcaagaccc
     1201 tgaagccgct gcctcgcgaa cgcactgacc gcagcatcgc cgccggcgcc gcgcagatag
     1261 ttgagggcat cgactgcgat gtcgtcatag gcgtgcggaa acatctcgcg accgtcgtcg
     1321 gtcagcgcga agagcttcgc cggccggccc cgaccgcgca gctgaccgtg caccttgcgc
     1381 tcgggaacct cactgacccg accggccgcg atcagcgcgt cgagatggcg cctgaccgcc
     1441 gctggggtga ggccgaagct cgcgctcagc gcgcctgcgc tctggggacc gtgctcgtgc
     1501 aacgccctgg ccacacggtc acgggtgcgc gcgtcatcag ggcccaagtg ggctccgggc
     1561 gaggcgtcag ccgcgagcgt tgatttcaca acagcaatgt tacctatttc gccccgagcc
     1621 cgcatcaccc ctccccgcgc tctgccgcca tcgccggtcg ggttcgcccc
//
LOCUS       DASWVF010000286         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_160559, whole genome shotgun sequence.
ACCESSION   DASWVF010000286 DASWVF010000000
VERSION     DASWVF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160559"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..233)
                     /locus_tag="VGD55_05085"
     tRNA            complement(163..233)
                     /locus_tag="VGD55_05085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Gly,seq:tcc)
     gene            276..348
                     /locus_tag="VGD55_05090"
     tRNA            276..348
                     /locus_tag="VGD55_05090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Pro,seq:tgg)
     gene            417..1757
                     /gene="tig"
                     /locus_tag="VGD55_05095"
     CDS             417..1757
                     /gene="tig"
                     /locus_tag="VGD55_05095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0869750.1"
                     /translation="MKSEVETLSPTRVKLTVEVDFDELTPSVDAAYRKVAGQIRIPGF
                     RPGKVPARIVDQRIGRDVVLDEAVNDAIPQFYGQAVEAAEVRVLSQPEIEVTHFADGE
                     QLTFTAEVDVRPAIELPELEGLPVTVDDADVADDDVDEQVKNLADRFGTLTSVERAVE
                     AGDFVSIDLSLSVGGEPLEDGTATGLSYEVGSDGLVEGLDSALLGLSAGESAEFDTAL
                     GGRGALVPTTGRALVTVRSVRTKQLPTIDDAFAQEASEFDTLNELRADLRTRLERVKR
                     LEQGMQARDKVLEALLAQVQIPLPERTVEAEIEGRRDALEQQLAANGLDLDAYLASQD
                     ETLEEHEAHIVVDVERDMRSQFVLDAVVAKEELGIEEAELTEYIVRRSSRAGINPDQY
                     AQQLVSSGTVPLAVADVLRGKALAFVLEHAVVTDASGRAVDLNRLEQDVTGPQD"
     gene            2041..2661
                     /locus_tag="VGD55_05100"
     CDS             2041..2661
                     /locus_tag="VGD55_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0869751.1"
                     /translation="MHSSTDPESRMPSSGVSPDDDVFNKLLKNRIVILGSVVDDAIAN
                     KICAQLLLLAAEDPEREIYMHINSPGGSVDAGMAIYDTMQYVPCDVATVGMGLAASMG
                     QFLLCAGERGKRYALPHARIMMHQPLGGIGGSASLIAIQAEQMLYTKKKLQELTAQHT
                     GQTVDQIERDSDRERWFSAEEAKDYGFVDHVVRSARQVPTETTSVS"
     gene            2678..3376
                     /locus_tag="VGD55_05105"
     CDS             2678..3376
                     /locus_tag="VGD55_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0869752.1"
                     /translation="MTQIQRPDLIDGDLFNAAGFNAERFNTERPNGRYIIPEFRERTS
                     TGDRIHNPYTKLFEERIIFLGVQIDDVSANDVMAQLLTLESMDPDRDIQLYINSPGGS
                     FTALTAIYDTMQFIRPEVQTICMGQAASAAAVILAAGAKGKRIALPNSRILIHQPSSE
                     AGGQASDIEIAAREILRMRALLEFMLAKHTGRTEAQVRIDIERDKILTAEEAVEYGLI
                     DEVIVSRKLTLAAH"
     gene            3509..>4271
                     /gene="clpX"
                     /locus_tag="VGD55_05110"
     CDS             3509..>4271
                     /gene="clpX"
                     /locus_tag="VGD55_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0869753.1"
                     /translation="MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELSESADLKWDELPKPREIYDFLEQYVIGQDIAKKALSVAVYNHYKRIQASGKE
                     SRAGADVVELAKSNILLIGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTTASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFS"
BASE COUNT          832 a         1419 c         1353 g          667 t
ORIGIN      
        1 agagcgggag cgtcgttgac gccgtctccg acgacaagga cacgcatctg actgtcctgc
       61 agtctcctga cccgatggac tttgtcctgc ggaagaagct cggagtgccc aaatggaggg
      121 ttgatggaga gaaacaggcc attgacctgc acctctttgc ggagcgggcg accgggatcg
      181 aaccggcatg gccagcttgg aaggctgggg ctctgccatt gagctacacc cgcgagtcgg
      241 tccgccgcgc ggcacctaga ctatcgggca cgccccgggg cgtagcgtag tggctagcgc
      301 gcctgctttg ggagcaggag atcgggagtt cgaatctccc cgccccgaca ggttccgcgt
      361 catccttcgg tagcctcgtc gtccacccgt atttctcgat cgaggagctg ctgtccgtga
      421 agagcgaagt cgagaccctg agccccacga gggtcaagct caccgtcgag gtcgacttcg
      481 acgaactgac cccgagtgtc gacgcggcct accgcaaggt cgccggccag atccggatcc
      541 cgggttttcg tcccggcaag gtcccggccc gcatcgtcga tcagcgcatc ggtcgcgacg
      601 tcgtgctcga cgaggccgtc aacgacgcca tcccgcagtt ctacggccag gccgtcgagg
      661 cggcagaggt gcgggtgctc agccagcccg agatcgaggt cactcatttc gcggacggcg
      721 agcagctcac gttcacggcc gaagttgacg tccgcccggc tattgaactg cctgaactcg
      781 aggggctgcc ggtcacggtt gacgacgccg acgttgccga cgacgacgtc gacgagcagg
      841 tcaagaacct ggccgaccgc ttcggcacgc tgacatcggt cgagcgggcg gtcgaggcgg
      901 gcgacttcgt cagcatcgac ctctcgctga gcgtcggcgg cgaaccgctc gaagacggca
      961 ccgccaccgg tttgtcctac gaggtcggca gcgacggcct ggtcgaaggc ctcgattcag
     1021 cactcctcgg cctctcggcg ggggagtcgg cggagttcga caccgcgctc ggcggtcgcg
     1081 gggcactggt cccgacgacg ggccgcgcac tcgtcaccgt tcgaagtgtg cgcacgaagc
     1141 agttgccgac catcgacgac gcgttcgcgc aagaggccag cgagttcgac acgctcaacg
     1201 agttgcgtgc cgacctgcgc acccgcctcg aacgggtgaa gcgtctcgag cagggcatgc
     1261 aggcacgcga caaagtgctc gaggcactcc tcgcgcaggt ccagatcccg ttgccagagc
     1321 gcacggtcga ggccgagatc gagggccgcc gcgacgcgct cgagcagcaa ctcgccgcga
     1381 acggcctcga cctcgacgcc tacctggcct cgcaggacga gacgctcgaa gaacacgagg
     1441 cgcacatcgt ggtcgacgtc gagcgcgaca tgcgttccca gttcgtgctc gatgccgtgg
     1501 tcgccaagga ggagctcggc atcgaagagg ccgagctcac cgagtacatc gtgcgccgtt
     1561 cctcgcgagc cgggatcaac cccgaccagt acgcgcagca gctggtgagc agcggcacgg
     1621 tcccgctggc cgttgccgac gtccttcggg gcaaggcgct cgccttcgtg ctcgaacacg
     1681 cggttgtcac cgacgcctcc gggcgcgccg tcgacctcaa ccgtctcgaa caggacgtca
     1741 ccggtccaca ggactgaccg ccgtccggcc taatgccctc ggccgtcggc gtgcgcaccc
     1801 acgccacggg cctgcgcaca ccacgccgtg ggcctgcgca caccacgccg tgggcctgcg
     1861 cccaccacgc cgtgggcctg cgcccatagc gaacagtgcg cgagagtctg ccgcgagact
     1921 cgatcggccg gttagggtcg gatagttggc acccgataaa gccgtgagca ggcagacacg
     1981 cctgtgcggc cgatctctgc gaaagatgag caggtgaaga gcgtggccct gaactacccg
     2041 atgcactcct cgaccgatcc ggagtcgcgg atgccatcgt ccggcgtgtc tcccgacgat
     2101 gacgtcttca acaagctgct gaagaacagg attgtgattc tcggctccgt cgtcgacgac
     2161 gcgatcgcca acaagatctg cgcgcagttg ctccttctcg ccgctgaaga tcccgagcgc
     2221 gagatctata tgcacatcaa ctcacccggc ggctcggtcg acgctggcat ggccatctac
     2281 gacaccatgc agtacgtgcc gtgcgacgtc gcgacggtgg gcatgggcct cgccgcatcg
     2341 atgggccagt tcctgctgtg cgctggcgag cgcggcaagc gctacgcgtt gccccacgcg
     2401 cgcatcatga tgcatcagcc gctgggcggc atcggcggtt cggcgtccct cattgcgatt
     2461 caagccgaac agatgctgta cacgaagaag aagctccagg agctcacggc ccagcacacg
     2521 ggccagaccg tcgaccagat cgagcgcgac tccgaccgcg agcggtggtt ctcagccgaa
     2581 gaggcgaagg actacggctt cgtagaccac gtggtgcgca gcgcccgtca ggtcccgacc
     2641 gagaccactt cggtctcctg agagcgagaa aacttccgtg acccagattc agcgacccga
     2701 cctcatcgac ggcgacctct tcaacgcagc gggcttcaat gccgagcggt tcaacaccga
     2761 gcgcccgaac ggccggtaca tcatcccgga gttccgcgag cgcacgagca ccggcgaccg
     2821 catccacaac ccgtacacca agctgttcga ggagcgcatc atcttcctcg gcgtccagat
     2881 cgacgatgtg tcggccaacg acgtcatggc ccaactgctg acgctcgaat cgatggaccc
     2941 cgaccgcgac atccagctct acatcaactc acccggtggc tcgttcactg cgttgaccgc
     3001 gatctacgac acgatgcagt tcatccggcc cgaggtgcag acgatctgca tgggccaggc
     3061 cgcatcggcg gcggccgtga tcctcgcggc aggcgccaag ggcaagcgca ttgcgctgcc
     3121 gaactcgcgg atcctgatcc accagccgtc gagcgaggcc ggcggccagg ccagcgacat
     3181 cgagatcgcc gcacgcgaaa tccttcgcat gagggcgctg cttgagttca tgctggctaa
     3241 gcacactggc cgtaccgagg cccaggtacg aatcgacatc gagcgggaca agatcctcac
     3301 agcggaagaa gccgtcgaat acggcttgat cgacgaggtc atcgtcagcc gcaagctcac
     3361 gctggcggca cactaagcac gcacgagaca cgcgataagt ggtccgaagc ctcggtcgag
     3421 gcggtacctt cggaggctgg acggcagcac ggacgcacga gcaggcatca ccccatacgg
     3481 tccgacggtc aggtaggaga cacatccggt ggcacgcata ggcgacggcg gtgacctgct
     3541 caagtgctct ttctgcggga agagccagaa gcaggtaaag aagctgatcg cgggccccgg
     3601 cgtctacatc tgcgacgagt gcatcgacct gtgcaacgag atcatcgaag aagagctgtc
     3661 ggagtcggcc gacctcaagt gggacgagct ccctaagccg cgcgagatct acgacttcct
     3721 cgagcagtac gtgatcggcc aggacatcgc caagaaggcc ctctcggtcg ctgtctacaa
     3781 ccactacaag cggatccagg cctcgggcaa agagtcccgc gccggtgctg acgtggtcga
     3841 gctggccaag tcgaacatcc tgctcatcgg cccgaccggt tgtggcaaga cctacttggc
     3901 gcagacgctg gcccgcatgc tgaacgttcc gttcgccatc gccgacgcca ccgcgctgac
     3961 cgaagccggc tacgtcggcg aagacgtcga gaacatcctc ctgaagttga tccaggccgc
     4021 tgactacgac gtcaagaagg ccgagaccgg catcatctat atcgacgaga tcgacaagat
     4081 tgcgcgcaag agcgagaacc cgtcgatcac ccgtgacgtg tcgggcgaag gcgttcagca
     4141 ggcactctta aagattcttg aggggacgac ggccagtgtg ccgccgcagg gtggacgcaa
     4201 gcacccgcat caggagttca tccagatcga cacgacgaat gtgctgttca tcgtcggcgg
     4261 agcattctcc g
//
LOCUS       DASWVF010000287         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_135409, whole genome shotgun sequence.
ACCESSION   DASWVF010000287 DASWVF010000000
VERSION     DASWVF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135409"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGD55_05115"
     CDS             complement(<1..317)
                     /locus_tag="VGD55_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY0869754.1"
                     /translation="MKILLVGAGGVGGAITGIAARRSFFDAIVVADYDEAKAQRAAER
                     TGDPRFSAIRIDASDEAAVAAALKEHGCDVLLNATDPRFVMPLFRAALSAGANYLDMA
                     MSLS"
     gene            complement(314..1372)
                     /gene="speB"
                     /locus_tag="VGD55_05120"
     CDS             complement(314..1372)
                     /gene="speB"
                     /locus_tag="VGD55_05120"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0869755.1"
                     /translation="MTRYGAMFGPDVTFLGVDRCDLDDLGSFAGADVVIVGAPFDGGT
                     SHRPGTRFGPQAIRSTDYLPHDGSRPHLALRVDALRDLRVLDAGDVEMPPGEIERALA
                     KLEDAVATVAVSGAIPIVLGGDHSIALPDITGVARHHGFGRVAVLHFDAHADTGNINF
                     GSLYGHGQPMRRLIESGAARGDRFLQIGLRGYWPEPETLDWMATHGMRSYEMTEIGAR
                     GLTAVLDEAFEIARDDCDAVFLSVDIDVCDPAHAPGTGTPEPGGLTARELLDAVRRIC
                     RELPIAGVDVVEVSPPFDHAEITAMLANRVVLEALSGLAAARRDAGEGVTFNPAAPLL
                     AGRLGEAKHHDSREGTTA"
     gene            1574..>2016
                     /locus_tag="VGD55_05125"
     CDS             1574..>2016
                     /locus_tag="VGD55_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869756.1"
                     /translation="MVAESTQVHYDNGEVLDKGLKGGALGLVSSIVIGVASTAPAYSL
                     AATLGFVAVLAGTQTVIVVILAFVPIFLISFGYKELNRREPDCGTTFTWGTKAFGPKT
                     GWMGGWGILAADILVMASLSQVAGQYFFLLVGAHGIGGNATSGWV"
BASE COUNT          366 a          666 c          656 g          328 t
ORIGIN      
        1 gacagcgaca tcgccatgtc gagatagttc gcgccggccg acagcgccgc ccggaacaga
       61 ggcatcacga agcgagggtc ggtggcgttg agcaagacgt cgcagccgtg ctctttgagc
      121 gccgccgcga cggctgcctc atcggaggcg tcgatgcgaa tcgccgagaa gcgcggatcg
      181 ccggtgcgct cggcggcccg ctgtgccttc gcctcgtcgt agtcggccac gacgatcgca
      241 tcgaagaacg accggcgggc agcgatgcca gtgatcgctc ccccgacgcc gcctgcacct
      301 accagcagga tcttcatgcc gttgtccctt ccctgctgtc gtgatgtttg gcttctccga
      361 ggcggcccgc gagcaagggt gccgctggat tgaacgtaac gccctcgccc gcgtcgcgcc
      421 gagccgccgc cagacccgac agcgcttcga gtacgacccg gttcgcgagc atcgcggtga
      481 tttcggcgtg atcgaacggc ggtgagacct cgacgacgtc gacgccggcg atcggcagct
      541 cacggcaaat tcggcggacg gcgtcgagca gctcgcgcgc ggtcagaccg ccgggctcgg
      601 gagttccggt gccgggagcg tgcgccggat cgcagacgtc aatgtcgacg gacagaaaca
      661 cggcatcgca gtcgtcgcgc gcgatctcga acgcctcatc gaggacggcc gtcaaaccac
      721 gcgcgccgat ctcagtcatc tcgtacgacc gcatgccgtg cgtggccatc cagtcgagcg
      781 tttcgggttc gggccagtag cccctcaaac cgatctggag aaagcggtcg ccgcgcgcag
      841 cgccggactc gatgagacgc cgcatgggct ggccgtgacc gtagagcgat ccgaagttga
      901 tgttgcccgt gtcggcgtgc gcgtcgaagt gcaggaccgc cacgcgaccg aatccgtggt
      961 ggcgtgccac accggtgatg tcgggcagtg cgatcgaatg gtcgccgccg agcacgatcg
     1021 gaatcgctcc cgacaccgcg accgtagcga cggcgtcctc gagcttggcc aaagcgcgtt
     1081 cgatctcacc cggcggcatc tcgacatcac ctgcgtcgag cacccgcaga tcccgcaggg
     1141 catcgacgcg caaggccagg tggggccggg atccgtcgtg tggcaggtag tcggtcgagc
     1201 gaatcgcctg cggaccgaac ctggtgcctg gacggtggga ggtgccgcca tcgaacggtg
     1261 cgccgacgat gacaacgtcg gcccccgcga acgatcccaa gtcatcgagg tcgcaacggt
     1321 cgacaccgag aaatgtgaca tcaggaccga acattgcccc atagcgggtc atttgctcac
     1381 ggtctcatga cagccagcat cggcgcctcg gccagctcgg gacaccagca tgcgcggacg
     1441 ctaccaccga aacggtagcc cgatcagatc gagaccacgg aatcccttgt caacagacca
     1501 aatcaatgcg ataaccggag ggttcaaaac cgctggtgcc ctcagtccgc ccccgtcgct
     1561 cccagaggtg ctcatggtcg ccgagtcaac acaggtgcat tacgacaacg gagaggtcct
     1621 ggacaaagga ctaaaaggcg gtgcacttgg tctcgtctcc agcatcgtca tcggggtcgc
     1681 atcgaccgcg ccggcctata gcctcgcggc aacgcttggg ttcgttgcgg tgctcgccgg
     1741 tacccagacc gtcatcgtcg tcattctcgc gttcgtcccg atcttcctga tctcgttcgg
     1801 ctataaggaa ctgaacaggc gcgagcccga ctgcggcacc acgttcacgt ggggtaccaa
     1861 ggcattcggg ccaaagacgg gctggatggg tggctggggc atcctcgccg ccgacatcct
     1921 ggtcatggcc agcctctcgc aggtcgctgg gcagtacttc ttcctgctcg tcggcgcgca
     1981 cggcataggt ggcaacgcga caagcggctg ggtgtt
//
LOCUS       DASWVF010000288         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_491809, whole genome shotgun sequence.
ACCESSION   DASWVF010000288 DASWVF010000000
VERSION     DASWVF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491809"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="ald"
                     /locus_tag="VGD55_05130"
     CDS             complement(<1..1098)
                     /gene="ald"
                     /locus_tag="VGD55_05130"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY0869757.1"
                     /translation="MKIGVPREVKNHEYRVAITPAGVHELVRAGHQVVVEKSAGVGSS
                     IPDEDFTSAGATIAPTADEVWDGADLVLKVKEPIAEEYHRMRKNQVLFTYLHLAASRP
                     CTDALIDSGTTAIAYETVQLPDRSLPLLAPMSEVAGRMAPQVGARCLEREAGGRGILM
                     GGVSGVYAAKVVVLGAGVAGMNAAAISLGMQAEVLLLDNNIEKLRQADRIYQGHLQTV
                     ASNSYEIEKACIDADLVIGAVLVVGAKAPKLIRDELVARMKPGAVLVDIAIDQGGCFE
                     SSRPTTHDAPTYRVHETLFYCVANMPGAVPHTSTYALTNVTLPYAMAIANLGWRDACR
                     ADPALALGLNVHEGAVVSAPVADAHDLDYRPL"
     gene            complement(1208..1843)
                     /locus_tag="VGD55_05135"
     CDS             complement(1208..1843)
                     /locus_tag="VGD55_05135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0869758.1"
                     /translation="MTEPVIDTAVIRRVVNSTSRFHGRVWDVRTDIVDLGDGQQVERD
                     VVQHPGAVGIVAVDEDLRVLLVHQYRHPVSAVLWEPPAGLLDIAGEDPLDAAGRELYE
                     EAAYRASQWSVLIDAFTTPGGSSESVRLYLARGLTPVAEADRHVGEGEERGMLTRWVG
                     LREVRAAVLAGELHSPLCIMGILAAASVLIDGVGQTRAVASPWLRSGSPST"
     gene            complement(1840..>2277)
                     /locus_tag="VGD55_05140"
     CDS             complement(1840..>2277)
                     /locus_tag="VGD55_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0869759.1"
                     /translation="TPHPVIATMADQRDVVAGERDMGGTMRLGSYPAELLEGSIVREM
                     YGEAYVKERHRHRYEFNNSYRAQLEAAGLVISGTSPDGRLVEFVELPREVHPYFVATQ
                     AHPELRSRPARPHPLFRGLVAAALERQAGETLLDIRTLPVATV"
BASE COUNT          418 a          784 c          702 g          373 t
ORIGIN      
        1 aagcggccga tagtcaagat cgtgcgcgtc agccaccggc gcgctcacca cggcgccctc
       61 gtgcacgttc agccccaatg ccagcgcggg atcggcccgg caggcatcgc gccatccgag
      121 gttcgcgatc gccatcgcgt acggcaacgt gacgttcgtc aacgcgtagg tcgaagtgtg
      181 tggcacggct cccggcatgt tcgccacgca gtagaacaac gtttcgtgca cccggtacgt
      241 cggggcatcg tgcgtggtcg gacgtgacga ctcgaagcac ccaccctgat cgatggcgat
      301 gtcgacgagc acagcacctg gcttcatgcg agccactagc tcgtcacgga tcagcttcgg
      361 tgcctttgcg ccgacgacga ggaccgctcc gatcaccagg tcagcgtcga tgcacgcctt
      421 ctcgatctcg taggagttcg aggcgaccgt ctgtagatgg ccctgataaa tccggtcagc
      481 ctggcgaagc ttttcgatgt tgttgtcgag cagcagcacc tccgcctgca tgccgagcga
      541 gatcgcagcg gcattcatgc ccgccacgcc cgcgcccaac acgacgactt tcgcggcgta
      601 cacgccggag acgccgccca tgaggatccc gcgaccaccg gcttcacgct cgagacaccg
      661 ggcgcccacc tgaggggcca tccgacccgc gacctccgac atcggcgcaa ggagtggcag
      721 cgaccgatca ggcaactgca ccgtctcgta cgcgatcgcg gtggtacctg agtcgatgag
      781 cgcatcggtg cacggccgcg acgccgccag gtgcaagtag gtaaatagca cctggttctt
      841 gcgcatgcgg tggtattcct cggcgatcgg ctctttgacc ttcagcacga ggtcagcgcc
      901 gtcccacacc tcgtcggcgg tcggagcgat cgtcgctcca gcagaggtga aatcttcgtc
      961 ggggatcgaa gagccgacgc cggccgactt ctcgaccacg acctgatgcc ccgcccgaac
     1021 gagttcgtgc accccagcgg gggtgatcgc aacccggtac tcgtggttct tgacttcacg
     1081 cgggacgccg atcttcacga cgtgctgcct tccgcctcga ccggcgagcg gcgccgtcgt
     1141 cgcccgagcg gtggcgcggt gtcgctcgcg ccagcaggag tctacgtccc agcgcgacct
     1201 acaccagtca ggtcgagggt gagccgctac gaagccacgg cgacgcaacg gctcgcgtct
     1261 gacccacgcc gtcgatcaac acactggctg cggccaaaat ccccatgatg cacagcgggc
     1321 tgtgcagttc gccggcgaga acagccgcgc ggacctcccg caagccaacc caccgggtca
     1381 gcatccctcg ctcttcgccc tcgccgacgt ggcggtcggc ctccgccacg ggcgtcagac
     1441 cgcgggcgag gtagagccgg actgattcac tcgacccgcc aggggtggtg aacgcatcga
     1501 tcaacaccga ccactgcgac gcgcgatacg cagcctcctc gtacaactcg cgcccagccg
     1561 cgtcgagcgg atcttcaccc gcgatgtcga gcaatccagc cgggggctcc caaagaaccg
     1621 ccgagactgg gtgccggtat tgatgcacca gcaacactcg gaggtcttcg tcgaccgcca
     1681 cgatgccgac cgcaccggga tgctggacca catcgcgttc gacttgctgc ccgtcgccga
     1741 gatcgacaat gtcagtgcga acgtcccaca cccggccatg aaaacgcgat gtcgagttca
     1801 ccacacgacg tatgacggcg gtgtcgatga ccggttcggt catacggtcg cgaccggcaa
     1861 ggtcctgatg tcgagcaagg tctcgccggc ctgccgctcg agtgctgcgg ctaccaggcc
     1921 gcggaacaac gggtgcggtc gcgccggtcg agaccgaagt tctggatgtg cttgcgtggc
     1981 aacgaaatac ggatgcactt cgcgcggcaa ctcgacgaac tcaacgagcc tgccatcagg
     2041 tgaggtcccg gaaatcacca gcccggcagc ctcgagttgt gcgcgataac tgttgttgaa
     2101 ctcgtaccga tggcgatggc gttccttcac gtacgcctcg ccgtacatct cgcgaacgat
     2161 cgagccctcg agcaattcag cgggatagga gcccagccgc atcgtcccgc ccatgtcgcg
     2221 ttcgccagcg acgacatcgc gctggtcggc catcgtggca atgaccggat gcggtgt
//
LOCUS       DASWVF010000289         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_160623, whole genome shotgun sequence.
ACCESSION   DASWVF010000289 DASWVF010000000
VERSION     DASWVF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160623"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGD55_05145"
     CDS             <1..401
                     /locus_tag="VGD55_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869760.1"
                     /translation="YRLYDIDRLISRTLSYTVVTGLLIGVYVGIVVLATDVLSFSSPV
                     AVAASTLAAAALFSPVRRAAQRVIDRRFNRASYDAELTVAAFTAKLREAVDLDTVRTD
                     LLTVISRAVEPTHAAIWISPARATRADSPS"
     gene            complement(361..1374)
                     /gene="hpnH"
                     /locus_tag="VGD55_05150"
     CDS             complement(361..1374)
                     /gene="hpnH"
                     /locus_tag="VGD55_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY0869761.1"
                     /translation="MAIPLRQGARIGWYLFSNKVRRVDKFPLIVELEPLFACNLKCNG
                     CGKIQQPHDLLAQRMPVERALAAIEEAGAPMVSLAGGEPLMHPQIDVMVNELVKRKKY
                     VYLCTNADLLRKRWDKFNFKPSPYFAFTSHIDGLQERHDESVAKQGVFEEAVEAIKFL
                     KEKGFRVTTNSTFFNTDTPQNIIDVLNFLNDEVKVDQMMISPAYAYEKAPDQDHFLGV
                     TETRELFTKAFAGGNRKRWRLNHSPLFLDFLEGKVDFECTAWGIPSYSLLGWQRPCYL
                     MADGYVKTYKELIETTDWSKYGRGRDSRCNNCMAHCGYEPTAVLATMGSMKESLRALR
                     EQG"
     gene            complement(1374..>2697)
                     /gene="ispH"
                     /locus_tag="VGD55_05155"
     CDS             complement(1374..>2697)
                     /gene="ispH"
                     /locus_tag="VGD55_05155"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0869762.1"
                     /translation="GDPTLRPGDVVVATEVRDDHGRIVQRAAAPLVAELRRMGLRVRT
                     GPMISCDQIVRGPEAMARLAETGAIAVDMESAAIVRAAAGVPTAVVRVIVDTAYSPVA
                     SLATIASGARALMTLRKLGPALRQWADLVGPRRVLLAAPRSFCAGVERAIDIVELALQ
                     RYPRPVYVRRQIVHNAHVVADLQRQGAVFVDELDQVPDGTTVVFSAHGVAPAVREEAA
                     RRGLNVIDATCPLVAKVHSEARRFVARGDTVVLIGHEGHDEVEGTMGEAPGRIKLVQN
                     VADAERVVASDPEHVAFLMQTTLAVDDATEAVSVLRRRFPAIESSATDDICYATTNRQ
                     QAVRAIADESDVMIVLGSANSSNSVRLVEVASRSGVPAHLVDDANDILPEWLSGASTI
                     GVTAGASAPPHLVDEVIETLRQLGSVDLVERAVAAEDVTFMLPKGVAG"
BASE COUNT          484 a          926 c          820 g          467 t
ORIGIN      
        1 agtatcgcct ttacgacatc gaccgcctga tcagccgcac gctctcctac acggtggtga
       61 ccgggctgct catcggcgtc tacgtgggta tcgtcgtgct cgcaaccgac gtgctgtcgt
      121 tctcatcacc agtcgccgtt gccgcgtcga cgctcgccgc agcagcactt ttcagccccg
      181 tccgtagagc ggcgcagcgg gtgatcgatc gccgtttcaa tcgcgcgagc tacgacgcgg
      241 aactaacggt ggcggcgttc acagcaaaac tgcgtgaagc ggtcgacctc gacaccgtgc
      301 gcaccgacct gctcaccgtg atcagccgcg cggtcgaacc aacccacgcc gcgatctgga
      361 tcagccctgc tcgcgcaacg cgcgcagact ctccttcata gagcccatcg tcgcgaggac
      421 ggcggttggc tcgtatccgc aatgagccat gcagttgttg catcgcgagt ctcgcccgcg
      481 gccgtacttc gaccagtcgg tcgtctcgat gagctccttg tatgtcttca cgtacccgtc
      541 tgccatcagg tagcagggcc gttgccaccc gagcagcgag tacgacggga tgccccacgc
      601 cgtgcactcg aagtcgacct tgccctcgag gaagtcgagg aacaacggtg agtggttcaa
      661 gcgccaccgc ttgcggttgc cgccggcgaa ggccttcgtg aacagttccc gcgtttcggt
      721 cacaccgagg aagtgatcct ggtcgggtgc cttctcgtag gcatatgccg gcgagatcat
      781 catctggtcg accttgacct cgtcgttgag gaagttcagc acgtcgatga tgttctgcgg
      841 agtgtcggtg ttgaagaacg tcgagttagt cgtgacccgg aagcctttct ccttcaagaa
      901 cttgatcgcc tcgaccgcct cttcgaacac gccctgcttc gcgacagatt cgtcgtgacg
      961 ttcctgtaga ccgtcaatat gactcgtgaa cgcgaagtac ggcgacggct tgaagttgaa
     1021 tttgtcccag cgcttgcgca gcaggtctgc gttcgtgcac aggtagacat acttcttgcg
     1081 cttgaccagc tcattgacca tcacgtcgat ctgcgggtgc atcaagggct caccgcccgc
     1141 gagcgacacc atcggcgcgc cggcttcctc gatcgccgcg agcgcgcgct cgaccggcat
     1201 ccgctgggcc agcaggtcgt gcggctgctg aatcttgccg cagccattgc acttgaggtt
     1261 gcaggcgaag agcggctcga gctcgacgat cagcggaaac ttgtcgaccc gacgcacctt
     1321 gttcgagaac aggtaccaac cgatcctcgc gccttggcgc agtggaatag ccatcagcca
     1381 gcaaccccct tcgggagcat aaaggttaca tcttcggcag caacagcacg ttcaaccaga
     1441 tcaacgctgc cgagctggcg caacgtttcg atcacctcat cgacgagatg aggcggtgca
     1501 gacgcgccgg ccgtaacgcc gatggtcgac gccccggaaa gccactcagg caggatgtca
     1561 ttcgcgtcat caacaagatg agccgggacg ccggagcgtg acgcgacctc gacaaggcgc
     1621 accgagttcg aagagttcgc agaccccagc acgatcatca cgtccgactc gtcagcgatc
     1681 gcgcgcaccg cctgctggcg gttcgtggtc gcgtagcaga tgtcgtcagt cgccgacgac
     1741 tcgatagccg gaaaacgtcg gcgcaagacc gagaccgctt cggtcgcatc gtcaaccgcc
     1801 aacgtcgtct gcatcaggaa cgccacatgc tcaggatccg acgcgacgac cctctcggcg
     1861 tccgccacgt tctgcacgag cttgatcctg cccggcgcct cgcccatcgt gccctcgacc
     1921 tcgtcgtggc cctcatgtcc aatgagcacg acggtgtcgc cgcgcgccac gaagcggcgc
     1981 gcctcgctgt ggaccttcgc taccagcggg caggtggcgt cgatgacgtt gagcccgcgc
     2041 cgcgcagcct cttcacgcac cgcgggcgcg acgccgtgcg ccgagaacac gacagtcgtg
     2101 ccgtcgggaa cctggtcgag ttcgtcgacg aataccgcgc cctgccgctg aaggtcggcg
     2161 acaacgtggg cgttgtgcac gatctggcgc cgcacataca caggccgtgg gtagcgctga
     2221 agcgccagtt cgacgatgtc gatcgcccgc tcgacgcccg cgcaaaacga tcggggcgcg
     2281 gccagcaaca ctcgccgcgg acccaccagg tcggcccatt gccgaagcgc cggcccgagc
     2341 ttgcgcagcg tcatcaacgc acgcgcgccc gacgcgatgg tggcgagcga tgcgaccggc
     2401 gagtaggccg tgtcgacgat cacccgaaca acagcagtcg ggacgccggc tgctgcgcgc
     2461 acgatcgcgg ctgactccat gtcgacggcg atcgcgccgg tctcggcgag ccgggccatc
     2521 gcttccgggc cgcgcacgat ctggtcgcag ctgatcatcg gcccggtccg cacccgcaac
     2581 cccatgcggc gcaactcggc aaccagcggc gcggccgccc gctgcacgat ccggccgtgg
     2641 tcgtcgcgca cctcggtcgc cacgaccacg tcgccggggc gcaacgtcgg atctccc
//
LOCUS       DASWVF010000290         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_186506, whole genome shotgun sequence.
ACCESSION   DASWVF010000290 DASWVF010000000
VERSION     DASWVF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186506"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VGD55_05160"
     CDS             complement(<1..1167)
                     /locus_tag="VGD55_05160"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0869763.1"
                     /translation="MATAAPTSTLASNELAIDVNAVLDVLGKVKAGDFGARLPFDWTG
                     VGGKLADGINEVISYNQALEAELARVSEVVGKEGRFSQRAALGGRWSGAVASVNSLID
                     DLVLPTIEMQRVIGAVADGDLSKKVSADVRGEMLELKNTINAMVVQLNGFSSEVTRVT
                     REVGTEGKLGQAAAVTIEVGGVWKDLTDNVNLMAGNLTGQMRNIAEVTTAVANGDLSK
                     KISVEARGEFLVLKNTINAMVDQLNAFSGEVTRVAREVGVEGKLGGQAQSKEVGGVWK
                     DLTDNVNQLAANLTNQVRAIAEVATAVTEGDLTRQVRVEASGEVAGLKDKLNEMIRNL
                     RETTQQNVEQDWLKTNREHFTRILQGQRDLSATAGMILSELAQLVSAQHAVFYSM"
     gene            complement(1372..1443)
                     /locus_tag="VGD55_05165"
     tRNA            complement(1372..1443)
                     /locus_tag="VGD55_05165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Val,seq:cac)
     gene            1648..2055
                     /locus_tag="VGD55_05170"
     CDS             1648..2055
                     /locus_tag="VGD55_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HEY0869764.1"
                     /translation="MVNVQTDNVVSNLEFSLVLVDADPITVAAHLRFDARDPFAVSVS
                     FEAGGHDRIEWTFARELLDQGLWAEIGDGDVRVWPRAGAVFLALCSPSGKAVLETRRP
                     AVADFLARIERIVPAGHESEFCDVDSELESLLS"
     gene            complement(2052..3089)
                     /locus_tag="VGD55_05175"
     CDS             complement(2052..3089)
                     /locus_tag="VGD55_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY0869765.1"
                     /translation="MEPFAHVDGRLATGLVEIRHDLDGLDGLDGLDSLDTSGFWVVVV
                     SFEGRVTCARFAEVHVAPLPAAAWQGVDPASWTSSLDRSAYVEGVEAIRRLIADGSVY
                     QVNLCRVLSAPTGAADLTGLANVLAAKHPSRYPALVCLPSEGIQVCSASPELFLSRGR
                     IGTDDVVQTRPIKGTGRTTRDISAKDRAENIMIVDMARNDLSRVARTGGVDVPALCEI
                     EEYPGLVHLVSTVQATLVPGVTWSEIFAATFPPASVSGAPKSSALRIIRDLEPAPRGP
                     YCGAVGWVDATTGSAALCVAIRTFWLDADQLRFGTGAGITWGSDPQAEWAETELKAEL
                     LLAAASSNVRR"
BASE COUNT          560 a         1046 c         1001 g          604 t
ORIGIN      
        1 catcgagtag aacactgcgt gctgcgcgga cacgagctgc gctagctccg acaagatcat
       61 gcctgccgtt gcgctcaggt cgcgctggcc ctgcaagatg cgcgtgaagt gctcgcggtt
      121 ggtctttagc cagtcctgct cgacgttttg ctgcgtcgtc tctcgaaggt tgcggatcat
      181 ctcgttgagc ttgtctttca agccggcgac ttcgccgctc gcctcgacgc gtacctgcct
      241 cgtgaggtca ccttcggtca ctgcggtggc gacctcggca atcgcacgca cctggttggt
      301 caggttggcg gctaactggt tcacgttgtc ggtcaggtct ttccagacgc caccgacctc
      361 cttcgactgt gcttggccgc cgagtttgcc ttcgacaccg acctcacgag cgacacgcgt
      421 cacttcacca gagaacgcgt tcagctggtc gaccatcgcg ttgatggtgt tcttcaagac
      481 gaggaactcg cctcgcgcct cgaccgagat cttcttgctc aggtcaccgt tggcgacagc
      541 cgtggtgact tcggcgatgt tacgcatctg cccggtgagg ttgccggcca tcaagttgac
      601 gttgtcggtc aggtctttcc agacaccgcc gacctcgatg gtgacggctg cggcctggcc
      661 gagcttgccc tcggtaccga cctcgcgagt aactcgcgtg acttcggagc tgaagccgtt
      721 gagctgcacc accatggcgt tgatggtgtt cttcaactcg agcatttcac cgcggacatc
      781 agcggagact ttcttgctca agtcaccgtc ggccaccgcg ccgataacgc gctgcatctc
      841 gatggtcggt agcacgaggt cgtcaatcag actgttaacg gacgcgaccg cacccgacca
      901 ccgtccgcca agggcagctc gctgcgagaa cctgccttcc ttgccgacca cctcgctcac
      961 cctggccagt tcggcctcga gcgcttggtt gtagctgatc acttcgttga tgccatcggc
     1021 gagctttccg ccgacaccgg tccagtcgaa cggcagccgt gcgccgaagt cgccggcctt
     1081 caccttgccg agcacgtcca gcacagcgtt gacatcgatg gcgagctcgt tacttgcaag
     1141 cgtcgatgtg ggcgcagctg ttgccatcgg ggagccctcc ggtggagatg tgtcacggcc
     1201 gcgactcccg cggcgcggtc gatcctgagg gacacgtcga cagccgcgac gaacaatcaa
     1261 ggaaaattgc ccaaatcgga cttaggcacg tggagacctg ccttaggtcc cgaatcgggc
     1321 gatcggtgat gccgaggact cgatactcgt tgtggtggtc gcaattgctt gtgggcgtac
     1381 ctggtttcga accagggacc tctgccgtgt gaaggcagcg ctctcccgct gagctatacg
     1441 cccgggaggc gatcactcta cacgcgagcc cctgagcagc ccggcggaaa tctcttggga
     1501 cgagtgtgcc ttcggcgggt taaaacctcc catgctgggg atctttgtct cagtgctctc
     1561 cgcgaccccg aggtcacaca cagctacgac gttcggcgcc tcaggcgcca gacggccaag
     1621 gatgagtcaa cgagcagccg aggaccggtg gtaaacgtgc agaccgacaa tgtggtctcc
     1681 aacctcgaat tcagtctcgt gcttgtcgat gccgacccga tcacggtcgc cgcgcatttg
     1741 cgcttcgacg cccgcgaccc gttcgcggtg tcggtcagct tcgaggcagg cggccacgat
     1801 cggatcgagt ggacgttcgc gagggaactc cttgatcagg gcctatgggc cgagataggc
     1861 gacggcgacg ttcgagtctg gccacgtgcg ggcgccgtct tcctggccct gtgctcccct
     1921 tcgggcaaag cggttctcga aacccgccgc ccagccgtcg ctgacttcct tgcgcgcatc
     1981 gagcggatcg ttccggccgg acacgaatcc gaattctgcg acgtcgacag cgagctcgag
     2041 agcctgctct cctaacgccg gacgttcgac gaagcggccg ccagcagcag ctccgctttc
     2101 agttcggtct cggcccattc agcttgtgga tccgaacccc acgtgatgcc cgcgcctgtc
     2161 ccaaaacgta actggtcggc gtccaaccaa aacgttctga tggcgacgca caacgcagcc
     2221 gaaccggtcg tggcgtcgac ccacccgacg gcgccgcaat aggggccgcg cggtgccggc
     2281 tcaaggtcgc gaatgatccg cagcgcactc gatttcgggg cgccggatac tgatgcgggc
     2341 gggaacgtcg cggcgaagat ctcgctccac gtgacaccag gaacaagcgt tgcctgcaca
     2401 gtcgagacaa ggtgcacgag tcctgggtac tcctcgattt cacacaaggc cgggacgtcg
     2461 accccgccgg ttcgtgcgac gcggctgaga tcgttgcggg ccatgtcgac gatcatgatg
     2521 ttctccgcgc gatccttcgc cgagatatct cgcgtcgtgc ggccggttcc cttgatgggc
     2581 cgcgtttgca cgacgtcgtc agtaccgatg cgcccgcgtg acaagaagag ttcggggctg
     2641 gccgagcaga cctgaatacc ctccgatggc aggcacacga gtgccgggta gcgcgacggg
     2701 tgctttgcgg ccaagacgtt cgccaagccg gtgaggtcgg cggcgccggt cggcgcggag
     2761 agcacccggc agaggttgac ctgataaacc gagccgtcgg cgatcaagcg gcgaatcgcc
     2821 tcgacccctt cgacgtaggc acttcgatcc agtgacgatg tccagctagc tgggtcgacg
     2881 ccttgccagg cagcagccgg caacggcgcg acgtgaacct cggcaaatcg cgcgcacgtc
     2941 acgcgtcctt cgaagctcac gacgacgacc cagaacccgc tggtgtcgag actgtcgagg
     3001 ccatcgaggc catcgaggcc atcgaggtcg tgccggatct cgaccaggcc ggtcgcgagt
     3061 cgaccgtcga cgtgcgcgaa gggctccact ttggtagccg ctagcccggc ggccgaagcg
     3121 ccaagccagc ggccacctcg agcagtacga gcgctgccaa acgaaccgtg cggtggtcgg
     3181 ccgcatcggc ggtggcatcg acctcgacga t
//
LOCUS       DASWVF010000291         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_237429, whole genome shotgun sequence.
ACCESSION   DASWVF010000291 DASWVF010000000
VERSION     DASWVF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237429"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VGD55_05180"
     CDS             complement(<1..820)
                     /locus_tag="VGD55_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0869766.1"
                     /translation="MTSRSSANSTSPDGTTTPGTTSHSTTTSPTTDSLTHRQAVGGVL
                     PLDPAVAVVVAVLTYRRPSELALLLPELSRQAAGRPAIRVLVVDNDPAASGSAVVSAA
                     ADPTIRYVHEPRPGIAAARNRALDEAASYDLLVFIDDDEWPNPTWLESMLEVHDRTAS
                     AAVVGPVISEYAAEPDPWIKAGRFFDRRRLPSGTRIDVAATNNLLLDLRQVRAFGLRF
                     DEAFGLSGGSDTLFTRELHRRGGALVWCDEAIVIDQVPMERLTRRWVLLRALRSG"
     gene            complement(862..>1731)
                     /locus_tag="VGD55_05185"
     CDS             complement(862..>1731)
                     /locus_tag="VGD55_05185"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0869767.1"
                     /translation="SSNLVASFNAGASSDSDGTIASYAWNFGDGSTGTGVAPTHTYAT
                     GNTYSVTLTVTDNGGATGTVTNPITVAAQGGITPFATDSFGRTVTGGWGTADTGGAWT
                     ISGGAANFSVGSAVGNINLATAGMSASSSLTSVTSTDTEVRVGVALNEAQTGGGTYAS
                     VIGRRISSTSDYRVKLKFASTGVVTEVLESNVGGTETALKTVTVPGVTYTPGMVLNVR
                     LQVTGTSPTTLNSMVWANGTTQPTTWQATATDSTASLQVAGAVGLLAYLSGSSTNPPE
                     TAAFNTFWAGPTH"
BASE COUNT          303 a          548 c          585 g          295 t
ORIGIN      
        1 tcccgctgcg caacgcgcgc aacagaaccc accgccgggt caagcgctcc atcgggacct
       61 ggtcgatcac gatggcctcg tcgcaccaca caagcgcgcc gccgcggcga tgcagttcgc
      121 gagtgaacag tgtgtcggat ccgccgctca aaccaaacgc ctcgtcgaag cgcaagccga
      181 acgcacgcac ctgtcgaagg tcgagcagca agttgttggt ggcggcgacg tcgatccgtg
      241 tgcctgacgg gagacgccga cggtcgaaga atcggccggc cttgatccac gggtctggct
      301 cagcggcgta ctcggaaatc actggaccga cgaccgctgc actcgccgtc cggtcatgaa
      361 cttcgagcat ggactcgagc cacgttggat tcggccattc gtcgtcatcg atgaagacga
      421 gcaagtcgta gcttgcggct tcgtcgagcg cgcggttgcg ggccgcggcg atgccgggcc
      481 ggggctcgtg cacgtagcga atcgtcggat cggcggctgc ggaaacgacc gccgagccac
      541 tcgcggcggg atcgttgtcg acaaccagca cccgaatcgc agggcgacca gcggcttgcc
      601 tcgacaactc gggcagcagg agtgcgagct cagaggggcg acgataagtc agcactgcca
      661 cgacaaccgc tactgccggg tcgagcggta ggacaccacc gacagcctga cggtgggtca
      721 ggctgtcggt ggttggactg gtggtggtgc tgtgactggt ggtgccggga gtagtggtgc
      781 catcaggact ggtgctgttg gcagaactgc gcgacgtcac gaggaacatc ccatcacgag
      841 atgacagcgg gcgagtcagg actagtgggt cggaccggcc cagaaggtgt tgaacgcggc
      901 cgtctcgggc gggttggtcg agcttcccga gaggtacgcg agcaacccga ctgcgccagc
      961 aacctgcaag ctcgccgtgc tgtccgtggc agtcgcctgc cacgtggtcg gctgcgtcgt
     1021 gccgttcgcc cagaccatcg agttcagcgt ggtcggcgac gtgcccgtca cctgtagccg
     1081 gacgttgagc accatgcccg gcgtgtaagt aacaccaggc accgtgaccg tcttcaacgc
     1141 cgtttcggtg cctccgacgt tcgactcgag cacttcggtc accacgccgg tcgacgcgaa
     1201 cttcagcttg acccggtagt cactggtcga gctgatgcgc cggccgatca cggaggcgta
     1261 cgtgccgcca ccggtctgcg cctcgttcag cgcaaccccg acgcgcactt cggtgtcggt
     1321 tgacgtcacc gaggtcagtg acgacgacgc actcatgcct gcggtcgcga ggttgatgtt
     1381 gccgacggcc gatcccacgg agaagttggc agccccaccg gagatggtcc acgcgccacc
     1441 ggtgtcagct gtgccccaac cgcccgtcac cgtgcggccg aacgagtcgg tcgcgaacgg
     1501 tgtgatcccg ccctgagcgg cgacagtgat cgggttggtg accgtgccag tagcaccgcc
     1561 gttgtcggtg acagtcaggg tgacgctgta cgtgttgccg gttgcgtacg tgtgggtcgg
     1621 tgcaacgccc gtacccgtcg agccgtcgcc gaagttccat gcgtacgacg cgatggtgcc
     1681 gtcgctgtcg ctggatgctc ctgcattgaa acttgcaacc aagttggacg a
//
LOCUS       DASWVF010000292         5104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_288273, whole genome shotgun sequence.
ACCESSION   DASWVF010000292 DASWVF010000000
VERSION     DASWVF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288273"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="VGD55_05190"
     CDS             complement(<1..1911)
                     /locus_tag="VGD55_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0869768.1"
                     /translation="MAGSATSTQALRRPGLRSLPRPRWDAVAKRSIRAAIVIPGLFAF
                     ADKVIKNPNVATFASIGSFSVLLLADFGGPLRRRLIAYASLAVAGAVFITIATLCSRT
                     TWLAVLVAGVVAALVLFLGILSGYVAKASTACLLILVLPLTLPASANAIPPRLEGWAL
                     ASTVGTLAVFILWRDPPQAGLGTLAGNALQRLADHLRATLDGTPPEEGAVAVETAVAK
                     LRTAYLATPFRPTGATLSEQAIVALVEEVDWLETLTAKHDDLPADGYARAATEDRELL
                     MSSVTALHDAGTALRDADAGSTARVACKPCDEPALVIDLELLGELRLQAAAATSQRVN
                     AWSAASIEADADVLECAFHARSVATATATAVSSALVASGNADHDAVRAEQSRWYGAAE
                     GSQSGVGELARQALAQTSLRSVWLRNSLRGAIGFAIAVLVAKETGVQHGFWVVLGTLA
                     VLRSNALGTGATALRALAGTSLGFIAGGALLVGIGHNTTALWIVLPFAVLLASYAPLR
                     ISFLAGQAAFTVLLAVLFNIVAPTGWRVGLVRVEDVAIGCAVSLVAGFLLWPRGAGPV
                     VSVDLADAYRTGVTYLSSAVHSLIGQHATLGQQAVSESAAGDATAASRRLDVAFRVYL
                     AEQGTKLVHRDEL"
     gene            complement(1981..>5104)
                     /locus_tag="VGD55_05195"
     CDS             complement(1981..>5104)
                     /locus_tag="VGD55_05195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0869769.1"
                     /translation="RAVEHSMHDSLTGLPNRTLLAERFQRALHAADRGDTTALLLIDL
                     DRFKEINDTFGHKYGDQLLNQIGRRFAAVIGVRDTVARIGGDEFAVLLPHIKDSAAAI
                     EIAECLRHSLDASFQVEGVELDVEASIGVVLSGEHGADAETLLQHADIAMYVAKTRNI
                     GVFAYSTDIDGYSPERLALLGDLRRAMEMHEIVLHYQPKVSVATGEVVGVEALARWQH
                     PTRGLVFPDAFIPAAEHTGLIGPFTSYVLDAALAQARIWADQERPLNVSVNLSGRNLL
                     DEHLPAEVADLLALHGVPASLLELEVTESAIMLDPVRARRLLQSLSDLGVRLSIDDFG
                     AGYTSLSQLKTLPVDELKIDRSFVMTMSSDARDALIVHSVIELGQNLGLNIVAEGVET
                     PEALVALRGFGCDIAQGYYIARPADVETFNAWLMARANAGPELAAATPARRAGDALLV
                     ERDLAEAMSPAGALRASEERFRALFTLAPIGIAEARADGTIVAMNPRACEMLGYQPDE
                     LIGRPAMMLIDPDDRAEQAQSMAALSKSDGYTARRVYRRKDGTSLTVLISVAVVRQAS
                     GAVYRIVGMMVDISELVAAQQSIAAAAVELAAHQMFTESLLASAGAGIIACDSTGVLT
                     VTNRTARSWHGLDPDIEPTPMQLAHLKADLYETDGVTPLALGSNPLRRAFRDSAIWTG
                     EMVIAPANAAATRVIATGTPLYGPTGESVGAVITMHDVTLLRQRESELDESAQFHDAI
                     LAASPDVIFVADAVTSRNVWSSRSLTEYGYSDQQVKDLGDDIIDRLVHPDDRLRVREQ
                     NATARTLPNGQVVRIRYRIRVENDTYRWFARNITPFTRNGSGQVTQLLGVASDVTELV
                     QVEQRFADAALRDSLTGSPNRALLADRLGKTLARTGRSGHQLAVLFCDLDGFKIVNDH
                     GGHEAGDAVLQATATRLLATLRAEDTAARVGDDKFVVILEPSSRTWAGPDGAPIDVRA
                     YAVQVAERIELALAVPVDFDGRAYAVTVSIGLVFAHAGDDVDEVLAEAGAAMHRAKGL
                     GKNRHEIATG"
BASE COUNT          892 a         1633 c         1709 g          870 t
ORIGIN      
        1 aagctcgtcg cgatgcacga gcttggtgcc ctgttcggca aggtagacgc gaaacgccac
       61 gtcgagccgg cgcgacgccg ctgtcgcatc acccgcagca gactcgctga ccgcctgttg
      121 tccgagcgtc gcatgctggc cgatgaggga gtgcacagca gacgacagat aggtgacgcc
      181 ggtgcgatac gcatcagcga ggtcgacaga cacaacgggt ccggcgccgc ggggccacag
      241 caagaagccg gcgacgaggc tcactgcaca tccgatcgcg acgtcttcta cgcgcacgag
      301 tccgacccgc cagccggtcg gcgccacgat gttgaacaag accgcgagga gcactgtgaa
      361 cgcggcctgc ccggcaagga acgagatgcg aagcggtgcg taactggcga gcagtaccgc
      421 gaacggcaac acgatccaga gcgcggttgt gttgtgaccg atgcccacca gcaaggctcc
      481 gccggcaatg aaacccagtg aggtgccggc gagtgcgcgc agtgcggtgg cacccgtgcc
      541 cagtgcgttc gaccgcaaca ctgcgagcgt gccgagcacg acccaaaacc cgtgctgcac
      601 acccgtttcc tttgccacca acaccgcgat cgcaaagccg atcgcaccgc gcaggctgtt
      661 gcgcaaccac accgaccgca ggctggtctg ggcgagcgcc tgccgggcca gttcgcccac
      721 gcccgactgc gatccttcgg ctgcgccgta ccaccgagac tgctcggcgc gcacggcatc
      781 gtgatcggca ttgcccgagg caacaagggc agacgacacc gcagtggctg ttgcggtagc
      841 gacggaccga gcatgaaaag cacactcgag cacatcggcg tccgcctcga tgcttgcagc
      901 agaccaggcg ttgacccgtt gcgacgttgc ggccgcagcc tggaggcgca actcgccgag
      961 caactcgaga tcgatgacaa gcgccggctc gtcacacggt ttgcatgcaa cccgggctgt
     1021 gctgccggcg tcggcatcgc gaagtgctgt tcccgcgtcg tgcaaggctg tcaccgacga
     1081 catcagtagc tcgcggtcct cggttgcagc acgcgcgtac ccgtcggctg gcagatcgtc
     1141 gtgcttggca gtgagtgttt cgagccaatc gacttcttcg acgagggcga caattgcctg
     1201 ctcagacaac gtcgctccgg tgggccgaaa cggcgtcgcc aggtaggcgg tgcgcagctt
     1261 cgcgaccgcc gtctcaactg cgacggcgcc ttcttctgga ggcgtgccgt ctagggtcgc
     1321 tcgcagatga tcggcgagtc gttgcagggc attccccgct agcgtgccga gcccggcttg
     1381 cggtggatcg cgccagagga tgaacacggc cagcgttccg acggtcgatg cgagtgccca
     1441 gccttcgaga cgcggtggta tcgcgttggc actggccggg agcgtgagcg ggagtacgag
     1501 gatcagcagg cacgcggtcg acgctttcgc gacgtacccg ctcaggatcc cgaggaacag
     1561 cacgagggcc gccacgacgc ccgcgacgag cacggcgagc caggttgtgc gcgagcagag
     1621 cgtcgcgatc gtgatgaaca cggcgcccgc gacggccagc gatgcgtacg cgatgagccg
     1681 cctgcgcagc gggccaccga agtcggcgag cagcagcacc gagaaactgc cgatcgatgc
     1741 gaacgttgcc acgttcggat tcttgatgac cttgtcagcg aatgcgaaca gcccgggtat
     1801 gacgatggca gcgcggatcg aacgtttcgc gacggcgtcc cagcgcggcc gcgggagact
     1861 ccgcaatccc ggacgtcgca gcgcttgcgt cgatgtcgcc gaacctgcca tggacgcagt
     1921 ccacaccacc taccggcggg ccagcaaccc cggggtcggc aatcagcgag caggtgtgcc
     1981 tcagccggta gcgatctcat gacggttctt gccaagcccc ttggcgcggt gcattgcggc
     2041 gcccgcttcg gccagaacct cgtcgacgtc gtcgccggcg tgggcgaata ccagcccgat
     2101 gctgacggtc accgcgtatg cgcgcccatc gaaatcgaca ggcactgcga gtgcaagctc
     2161 gatgcgctcg gcgacttgca cggcataggc acggacgtcg attggcgcgc cgtcgggccc
     2221 cgcccaggta cgagatgacg gctcgaggat gacgacgaac ttgtcgtcac cgacgcgcgc
     2281 agcggtgtct tccgcgcgaa gcgtggcgag cagccgggtg gctgtcgcct ggagcacggc
     2341 gtcgcccgcc tcgtggcccc cgtgatcgtt gacgatcttg aacccgtcga ggtcgcagaa
     2401 gagtacggcg agttgatggc cactgcggcc cgttctggcg agcgtcttcc cgagccgatc
     2461 ggcgagcagc gctcggttgg gcgagccggt gagggagtcg cgaagagctg cgtcggcaaa
     2521 ccgctgctcg acctgcacga gctctgtcac atcactcgcg acaccgagga gctgggtcac
     2581 ctggccgctg ccatttcgcg tgaagggcgt gatgttgcgg gcgaaccagc ggtaagtgtc
     2641 gttttcgacc cttatccgat aacggatccg tacaacctgg ccattgggca atgtccgcgc
     2701 ggtggcgttc tgttcgcgaa ccctgaggcg atcgtcgggg tgcacgagcc gatcgatgat
     2761 gtcgtctccg agatccttca cttgctgatc ggagtagccg tactcggtga ggcttcgtga
     2821 cgaccacaca ttgcgactcg tgaccgcgtc ggccacgaag atcacatcag ggctggctgc
     2881 aaggatcgca tcgtggaact gggcgctctc gtcgagctct gattcgcgct ggcgcaacaa
     2941 cgtgacgtcg tgcatggtga tcaccgcacc gaccgactca ccggttggcc cgtaaagcgg
     3001 cgtgccggtt gcgatcactc gggtcgccgc tgcgttggcc ggtgcgatca ccatctcgcc
     3061 cgtccagatc gcgctgtctc gaaacgcgcg ccgcagcggg ttgcttccta aggcaagcgg
     3121 cgtgacgccg tcggtttcgt agaggtcggc cttcagatgc gcgagttgca ttggagtcgg
     3181 ctcgatgtcg gggtcaaggc cgtgccagct tcgtgcggtg cggttcgtga ctgtcaacac
     3241 tccggtgctg tcgcacgcga tgatcccggc gcccgcgctg gcaaggagag attcggtgaa
     3301 catctggtga gcggccagct cgacagctgc cgcggcgatg ctttgttggg ccgccaccag
     3361 ttcgctgatg tcgaccatca tgccgacgat gcgatagacg gctccggagg cttggcgcac
     3421 gacggcgaca ctgatgagga ccgtcaacga cgtgccgtcc ttgcgccggt agacgcgtcg
     3481 ggccgtgtat ccgtcggact ttgacaatgc ggccatgctc tgagcctgct cggcccggtc
     3541 gtcggggtcg atcagcatca ttgccggtcg cccgatcagc tcgtcaggct ggtagccgag
     3601 catctcgcac gcccgcgggt tcatcgcaac gatggtgccg tcggcacgtg cctccgcgat
     3661 cccgatcgga gcgagcgtga agagcgcacg gaagcgctcc tcgctggctc gcaaagctcc
     3721 agcgggcgac atcgcctcgg caaggtcacg ctcaacgagc aaggcgtcgc ccgcgcgccg
     3781 cgccggggtc gcagctgcta gctcaggacc agcgttcgct cgtgccatca gccaagcgtt
     3841 gaaggtctcg acgtccgcag gccgagcgat gtagtaaccc tgggcgatgt cgcaaccgaa
     3901 gccacgcaag gctacgagcg cctcgggcgt ctcgacgcct tcggcgacga tgttgagtcc
     3961 aaggttttgc cccagttcga tcacgctgtg cacgatgagc gcgtcgcgtg cgtccgacga
     4021 catcgtcatc acgaacgacc ggtcgatctt cagttcgtcg accggcagcg tcttcagttg
     4081 gctcaggctc gtgtagccgg caccgaagtc gtcaatcgac aaccggacgc cgagatccga
     4141 caggctttgc agaaggcggc gggcgcgcac cgggtcgagc atgatggccg attcggtgac
     4201 ttcgagctcg agcagtgatg ccggcactcc gtgcaatgcg agcaggtcgg ctacctcggc
     4261 tggcaggtgc tcgtcgagca agttgcgccc cgacaagttg accgacacat tcaggggtcg
     4321 ctcctgatct gcccagatcc gggcttgcgc caacgccgcg tcgagcacgt agctggtgaa
     4381 gggcccgatc aagccggtgt gttctgcggc ggggatgaag gcgtcaggga acaccaggcc
     4441 gcgtgtcggg tgctgccagc gcgcgagtgc ctcgacccca acgacctcgc cggtcgcgac
     4501 gctgaccttc ggctggtagt gcagaacgat ctcgtgcatt tccattgccc gccgtaggtc
     4561 gccgagcagc gcgagtcgtt cgggggagta cccatcgatg tcagtgctgt acgcgaagac
     4621 gccgatgttg cgcgtcttgg cgacgtacat cgcgatgtcg gcgtgctgaa gcaacgtctc
     4681 ggcgtcggcg ccgtgctcgc ccgacagcac gacaccgatg ctggcctcga catcgagttc
     4741 gaccccctcg acctggaatg acgcgtcgag tgagtgtcga aggcattcgg cgatttcgat
     4801 cgctgcggcg ctgtctttga tatgaggaag aagcactgcg aactcgtctc cgccgatgcg
     4861 cgcgactgtg tcacgtacgc cgatgacggc cgcgaatcgg cgtccgatct gattcagcag
     4921 ttgatcgccg tacttgtgcc cgaacgtgtc gttgatctcc ttgaaccggt cgaggtcgat
     4981 caacagcagc gcggttgtgt cgccacgatc cgcggcgtgc agtgcgcgtt ggaagcgttc
     5041 ggcgagcagt gttcggttcg gcaggcccgt cagcgaatcg tgcatcgagt gttcgacggc
     5101 gcga
//
LOCUS       DASWVF010000293         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_186553, whole genome shotgun sequence.
ACCESSION   DASWVF010000293 DASWVF010000000
VERSION     DASWVF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186553"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGD55_05200"
     CDS             complement(<1..1139)
                     /locus_tag="VGD55_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0869770.1"
                     /translation="MTALGKLLRTTAFQLTLVYLVVFALFAAFLVGYFALNTRRLINE
                     QIVTTVDTDVTGLTNQYSQAGIRRLVVIVDVRARRPGSSLYLVTTPTGEGLAGNVGSL
                     EPGILDHDGWVETAYRRLEEPESTEHNALVRVTRLPGGIRLLVGRDLEERERMFHIVA
                     VAGRWSVALVVVLGVLGGIFVSRRVLNRVEAMTATAQTIMAGDLSGRLSVTGSGDEFD
                     RLALNLNGMLERIESLMHGLKEVTDNVAHDLKTPLTRLRNRCEAALRIAKTDADYHHV
                     LEETIEESEGLIRTFDALLMIARAESGEIRDGMAEFDIADVARDVGELYEPLAEDKGL
                     TLQIEANEHATVKGNRELVSQALANLVDNAIKYAAPAAKQANGERA"
     gene            complement(1123..>1743)
                     /locus_tag="VGD55_05205"
     CDS             complement(1123..>1743)
                     /locus_tag="VGD55_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869771.1"
                     /translation="REVGHVADLAADGEEGLSLALDGGYDVMIIDRMLPKLDGLTVIG
                     RLRAKGNTAPALILSALGAVDDRVRGLRAGGDDYLPKPYSFSELLARVEVLARRHTGR
                     GEETQYRVADLELDRLSHRVVRGSEEISLQPREFRLLEYLMKHAGQVVTRTMLLENVW
                     DYHFDPQTNIIDVHISRLRSKIDKGFATPLLHTVRGAGYMIRDGAR"
BASE COUNT          271 a          582 c          589 g          301 t
ORIGIN      
        1 gcgcgttcgc cgttggcttg cttcgccgcg ggcgctgcgt atttgatcgc gttgtcgacg
       61 aggttggcaa gcgcttggct cacgagctcg cggttgccct tgacggtcgc gtgctcgttg
      121 gcctcgatct gcagcgtcag gcccttgtcc tccgccagcg gctcgtagag ctcgccgaca
      181 tcccgggcga cgtcggcgat gtcgaattcc gccatgccgt ctcggatctc gccggattcg
      241 gcccgcgcga tcatcaacag cgcatcgaag gtgcggatca acccttcgga ctcctcgatg
      301 gtctcctcga gcacgtggtg gtagtcggcg tcggtcttgg cgatccgcaa cgccgcctcg
      361 caacggttgc gcaaccgcgt cagcggcgtc ttgaggtcat gggcgacgtt gtcggtgacc
      421 tccttcaatc cgtgcatgag cgactcgatg cgctcgagca tgccgttcag gttgagcgcc
      481 aggcggtcga actcgtcacc gctcccggtc accgagagcc ggcccgacag gtcgcccgcc
      541 atgatggtct gggcggtcgc cgtcatcgcc tcgacgcggt tgagcacgcg gcggctcacg
      601 aatatgccgc cgagcacccc gagcacgacc acgagggcga ccgaccaccg gccggccacc
      661 gcgacgatgt ggaacatccg ctcgcgctcc tcgaggtcgc gtccgacgag aagcctgatg
      721 ccgccgggca ggcgcgtcac ccggacgagc gcgttgtgct cggtcgattc cggctcttcg
      781 agacgccggt aggcggtctc gacccagcca tcgtggtcga ggatgccggg ctcgagcgag
      841 ccgacattgc cggcgagccc ctccccggtc ggggtcgtga cgaggtagag gctcgagccc
      901 ggccgccgcg cgcgcacgtc gacgatcacc acgagccgcc ggatgccggc ctgactgtac
      961 tggttggtga gccccgtgac atcggtgtcc acggtggtga cgatctgctc gttgatcaac
     1021 cggcgcgtgt tgagggcgaa atagccgacc aggaaggccg cgaacagcgc gaacaccacg
     1081 agatagacga gcgtcagctg gaacgccgtc gtgcggagaa gcttaccgag cgccgtcacg
     1141 gatcatgtac cctgcccccc gcaccgtgtg cagcagcggc gtcgcgaagc ccttgtcgat
     1201 cttcgagcgc aaccgcgaga tgtgcacgtc gatgatattc gtttgcgggt cgaagtggta
     1261 gtcccatacg ttctcgagga gcatggtccg cgtcaccacc tggccggcat gtttcatgag
     1321 atattcgagc agtctgaact cgcgcggttg caaggagatt tcctcgctgc cgcgcacgac
     1381 gcggtgggac agccggtcga gttcgagatc ggcgacgcgg tattgcgtct cctcgccccg
     1441 gccggtgtgg cggcgcgcca gcacctcgac gcgcgccaac agctcggaga acgagtacgg
     1501 cttgggcagg tagtcgtcgc cgccggcacg caggccgcga acccggtcgt cgacggcacc
     1561 gagggcggag agaatgagcg cgggcgccgt gttgcccttc gcccgcagcc gcccaatcac
     1621 cgtgagcccg tcgagtttgg gcagcatccg atcgatgatc atcacgtcgt acccgccgtc
     1681 gagcgcgagg gagagtcctt cctctccgtc ggcggcgagg tcggcgacat ggccgacttc
     1741 gcg
//
LOCUS       DASWVF010000294         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_263140, whole genome shotgun sequence.
ACCESSION   DASWVF010000294 DASWVF010000000
VERSION     DASWVF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263140"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGD55_05210"
     CDS             complement(<1..823)
                     /locus_tag="VGD55_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY0869772.1"
                     /translation="MRETDALSNAVDALQQLDGSGLSDEALCEQVMALRKQIDRLESV
                     FTLRVGLMHERGSANGSGYVTTAAFLRHGCKLTAGAARSRVDTAALLRVWPMVASAFA
                     EGAISFPHTAMVTSTLTTLPAEVAVDAQPVLVEAAKLLDTRRLAQTVQRLRYIVDPDG
                     AAGLDERHHERRWLDVVATYRGTVAINGMLDAEAGAVLLTALEALMGPPAPDDDRTGA
                     QRRADALVGLVRTGLDHANLPDVGGERPHVIVVSTIESLRGDAGAPPSELGCAGSL"
     gene            complement(976..1626)
                     /locus_tag="VGD55_05215"
     CDS             complement(976..1626)
                     /locus_tag="VGD55_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0869773.1"
                     /translation="MSDTRVKLIDGAITTLRDKGIAGTSARVIAAAAGVNQALIFYHF
                     GSVDALIDVACREGTDARVALYRDQFSEVTSFRELLALGRELHARERDAGNVAVLAQV
                     LAGAQQDDNLAAAAQHALALWIAEIETTLTRLLKGSPIAALANAEGLARGIAASFIGI
                     ELFEGVDPDGAESALASLEMLGVLVEVVEDLGPVARRALRARLRRTERASNAAPSA"
     gene            complement(1623..>2087)
                     /locus_tag="VGD55_05220"
     CDS             complement(1623..>2087)
                     /locus_tag="VGD55_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869774.1"
                     /translation="RSVGFGVTSGAALGVAGFGVLAVYPWESPSFIALPVFDLFAAVI
                     GAVMGGLVGFICGLLLVVAGPMARGDGRTVRRISSIGACAPFVLLAVLDAENGGVAWE
                     LGGWFWWLIVAAMAFAAGAAIGPHVVGGRADPLAAGHSCFRHCRITRGVGPG"
BASE COUNT          433 a          696 c          622 g          336 t
ORIGIN      
        1 cgagagagcc ggcgcaacca agctccgagg gcggggcgcc ggcgtcaccg cgcaagctct
       61 cgattgtcga gacgacgatc acatggggac gctcgccacc aacgtcaggc aggttcgcgt
      121 ggtcgaggcc ggtgcgcacg agtccgacca atgcgtcggc gcggcgctgc gcgcccgtac
      181 gatcgtcgtc gggagccggt ggccccatca gcgcctcgag cgccgtcagc agaaccgcgc
      241 ctgcttccgc gtcgagcatt ccgttgatgg caacagttcc ccgatatgtg gcaacgacat
      301 cgagccaacg tcgctcgtga tgccgctcgt cgagccctgc tgcgccatcc ggatcgacga
      361 tgtagcgcag acgctggacc gtctgcgcca atcggcgagt gtcaagaagc ttcgctgcct
      421 cgaccagcac aggttgtgca tcaaccgcga cctcggccgg aagcgtggtc aacgtcgacg
      481 tcaccatcgc ggtatgtggg aaagagatcg caccttcagc gaatgcactt gccaccatgg
      541 gccatacgcg cagcaaagct gctgtgtcaa ctcgcgaacg agctgcgcca gcggtcagct
      601 tgcagccatg acgaaggaac gctgcggttg tgacgtatcc cgatccgtta gctgacccac
      661 gttcgtgcat caagccaacc cgcaaggtga ataccgactc gagccgatcg atctgcttgc
      721 gcagtgccat cacttgctcg cagagtgcct cgtcagaaag acccgaaccg tcgagctgct
      781 gcaacgcatc gactgcgttc gacagtgcat cggtttcgcg catcggttgg cccccgtttc
      841 ttcgttgaca tcaagctatc gaatgccgcc gacaaaaact gaactcacga tttgagtgtg
      901 tacaaagcta atttcagaga tgcgcaatgt ggagaactca ccgaagggtt cggccgtact
      961 cagccgcacg actcgctaag cgctcggcgc cgcattggaa gcacgctcgg ttcgtcgcag
     1021 tcgagcacgg agtgcgcgtc gcgcaaccgg ccccagatct tcaacgacct cgacaagcac
     1081 gcccagcatc tcaagagaag cgagcgcact ctcagcgccg tcggggtcaa cgccttcgaa
     1141 caactcaatg ccaatgaatg atgcggcaat gccacgagca agaccctctg cgttcgcgag
     1201 agccgcgatc gggctccctt tcaagagtcg cgtgagcgtg gtctcgattt cggcgatcca
     1261 cagcgcgagc gcgtgctgag ctgcagctgc gagattgtcg tcttgttgtg cacccgcgag
     1321 aacttgagcg agaacggcga cattgccggc gtcgcgttcg cgcgcgtgta gttcgcgacc
     1381 gagtgcaagc agttcacgaa acgacgtgac ctcgctgaac tgatctcgat agagcgcaac
     1441 tcgagcgtcg gtcccttccc tgcacgcgac atcgatcagc gcatcgaccg agccgaagtg
     1501 gtagaagatc agtgcttggt tcacccccgc cgcggccgcg atcacccggg ccgaagtgcc
     1561 agcgatccct ttgtcgcgca gggtcgtgat tgcaccgtcg atgagtttga cgcgcgtgtc
     1621 gctcatccgg gtccaacgcc gcgcgtgatg cggcagtgcc gaaagcagga gtggcccgcg
     1681 gcgagtggat ccgcccgacc accgaccacg tgaggcccga tcgctgcgcc cgccgcgaac
     1741 gccatcgcgg cgacaattag ccaccagaac caaccgccca actcccaagc cacaccgccg
     1801 ttctcggcgt cgagcacggc tagcagcacg aacggagcac atgccccgat gctcgagatc
     1861 ctgcgcactg tgcgaccgtc tccccttgcc attggcccgg caaccaccag cagaaggccg
     1921 cagatgaatc cgaccaaccc gcccatgacg gcgccgatga cagccgcgaa caggtcaaag
     1981 accgggagcg cgatgaaact cggagactcc catggataga cagccaatac cccgaaacca
     2041 gccacgccga gcgctgcgcc ggacgtgacg ccgaagccga ccgaacg
//
LOCUS       DASWVF010000295         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_237501, whole genome shotgun sequence.
ACCESSION   DASWVF010000295 DASWVF010000000
VERSION     DASWVF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237501"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VGD55_05225"
     CDS             <1..1411
                     /locus_tag="VGD55_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY0869775.1"
                     /translation="YNTLGFFAPHAGYSASGSGGEQVVEFKQMVKALHAAGLEVILDV
                     VYNHSADGDEFGPTLGFRGIDNASYYRLQDGRLYVNYTGCGNTLDVPNLHTLQLITDS
                     LRYWVTDMHVDGFRFDLASTLARSSDAFDARSSFLAALQQDPVLSQVKLIAEPWDIGP
                     GGYQVGNFPAPWTEWNDRFRNTTRDFWLRGMSDVRELGYRLSGSSDLYEASGRRPYAS
                     LNFVTCHDGFTLHDLVTYDNKVNDANGEGNRDGSDDNHNWNCGYEGETPDPVVNQLRA
                     RAMRGILGTLLVSAGVPMLGHGDEMGRAQRGNNNAYCQDNSLTWIDWAARDESLLAFT
                     QAAIALRRKHQVFRRTSFFTGTSSSDEGIKDLAWFSADGVEMAASDWYDLRTRSIAMY
                     LSGHDTGEVDAHGAPVVDDSFLVIMHTGLEPATFTLPGAAWATAYEVVLDSTETLGVA
                     GPGQPLDVPGVAFAVLRAT"
     gene            complement(1489..2397)
                     /locus_tag="VGD55_05230"
     CDS             complement(1489..2397)
                     /locus_tag="VGD55_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY0869776.1"
                     /translation="MEPLLVAIAAAQCGVFTRAQALAAQYSDKRIRHLVATGTWLRCH
                     AAIYCLAGVAPSLYGNAWVAVLAAGEDARLSHRMAGKLHVLEGTPPPVRFDVSVPAVR
                     VPRNVPRATIHRTELGPEDIAVCRGFPVTSIARTLVDLGRTLPLEIGSRVIGDALRTG
                     RVSADALEGRIVALARCVGIDRARRALASADPKLESVLELELFALLRRAGLDPVIQYV
                     VTVGGSFVARVDFALPGIRLAIEADGYDTHARRPGFERDREKAALLQLAGWWLLSFTA
                     TQIRQRPDWVAATVLTRVRQLTVAAG"
     gene            complement(2548..>3699)
                     /gene="glgP"
                     /locus_tag="VGD55_05235"
     CDS             complement(2548..>3699)
                     /gene="glgP"
                     /locus_tag="VGD55_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY0869777.1"
                     /translation="GAMESELGWINDVLDPDVLTIGFARRVPSYKRLTLMLQDPKRFT
                     ALLLDRDRPVQFVIAGKAHPADDGGKRLIQEVVKFADGAGVRHRVVFLPDYDMALARV
                     LCAGADVWLNNPLRPLEACGTSGMKAALNGVLNLSIRDGWWDEWFDGDNGWAIPSADD
                     LPEERRDEIEAGAFYDIVETQMVPRFYDHDGDGLPRRWLEMVRHTLSSLGPKVVATRM
                     LRDYVTNLYTPAAQSSRAMESAKFGGAKHLAAWKKRVTTAWPGVHVEHIEARGVSDAL
                     EYGTVVSLRAVVALGDLAADDIDVQVAYGRVDEHDQITGATYGSLRLSDKRDDGSWVY
                     EGEMTLDRTGPFGYSVRVLPSDALLVTAGELGLIAVPPVDEGMVTGDLR"
BASE COUNT          667 a         1238 c         1168 g          626 t
ORIGIN      
        1 ctacaacact ctcggcttct tcgcacccca cgccggctat tcggcgtccg gttctggtgg
       61 cgagcaggtc gtcgagttca agcagatggt gaaggcgctg cacgccgccg gcctcgaagt
      121 gatcctcgac gtcgtgtaca accacagtgc cgacggcgac gagttcggtc cgacgctggg
      181 atttcgaggg atcgacaatg cgtcgtacta ccggctccag gacggccgcc tctacgtgaa
      241 ctacacgggc tgcggcaaca cactcgacgt cccgaatctt catacgctgc agctgattac
      301 cgactcgttg cgctactggg tcaccgacat gcacgtcgac ggtttcaggt ttgatctcgc
      361 ctcgacgctc gcccgttcga gcgatgcatt cgatgcacgg tcgtcgtttc tcgcggcgtt
      421 gcagcaggat ccggtgctat cgcaggtgaa gctgatcgcc gagccgtggg acatcggccc
      481 cggcggatat caggtcggca acttcccggc accgtggacc gaatggaacg accgtttccg
      541 caacaccaca agggatttct ggctgcgtgg catgagcgac gtgcgcgagc ttggctaccg
      601 gctctctggt tcgtcagacc tctatgaggc gagtggccgc cgaccctacg cgtcgttgaa
      661 cttcgtcacg tgccacgacg ggttcacgct gcacgacctt gtgacgtacg acaacaaggt
      721 caacgacgcg aacggcgagg gaaaccgcga cggctctgat gacaaccaca actggaactg
      781 cgggtacgaa ggcgagacgc cagaccctgt cgtgaaccag ctccgcgccc gcgcgatgcg
      841 ggggattctc ggcacgctgc tggtgtcggc cggtgtgccg atgctcggac atggcgacga
      901 gatgggtcgc gcccagcgcg gcaacaacaa cgcctactgc caggacaact ccctcacctg
      961 gatcgactgg gcggcccgcg acgaatcgtt actcgcgttc acgcaagccg cgatcgcgct
     1021 gcgccgcaag caccaggtat ttcggcgaac gtcgttcttc acgggcacct cgagcagcga
     1081 tgagggcatc aaggatctgg cgtggttctc tgcagacggc gtggagatgg cggcatcgga
     1141 ttggtatgac ctgcgcaccc gctcgatcgc gatgtacctg tcaggtcacg acaccggcga
     1201 ggtcgacgcg cacggtgcac cagtcgtcga cgattcgttc ctcgtcatca tgcacaccgg
     1261 gctcgaacca gcgaccttca ctcttcccgg cgctgcgtgg gccacggcgt atgaggtcgt
     1321 actcgacagc accgagaccc tcggcgtggc ggggcccggc cagccactcg acgtccccgg
     1381 agtcgcgttc gccgtgctcc gcgccacctg aactcaccac cagtccgcac agcgtgtgcg
     1441 tttgggtgcc ccgcagggtg attcacgcac gcgctggggc gcagacggct agccggccgc
     1501 aacggtgagc tggcgcactc gcgtcaacac ggtcgcagcg acccaatcgg ggcgttgccg
     1561 gatctgggtc gctgtgaacg acaacagcca ccaaccggcg agctggagca gcgcggcttt
     1621 ctcccggtcg cgttcgaagc ctggacgccg tgcatgcgtg tcatagccgt cggcctcaat
     1681 cgccagtcgg atgcccggta gtgcgaagtc gactcgcgcg acaaaggatc cgcccactgt
     1741 cacgacgtac tgaatgacgg gatctaaccc ggcacggcgc aacagcgcga agagctccag
     1801 ttcgagcacg gattcgagtt tggggtcagc gctcgcgagc gcacgacgtg cccggtcgat
     1861 gccgacgcag cgggcgagtg ccacgatcct gccctccaac gcgtcggccg acactcgccc
     1921 ggttcgcagt gcgtcgccga tcactctcga tccgatctcg agtggcaagg ttctgccaag
     1981 gtcgaccaac gttcgggcga tcgaggtgac cgggaaacct cggcaaacgg caatgtcttc
     2041 gggtccgagt tcggtgcgat ggatcgtcgc cctggggacg ttgcgtggaa cacgaaccgc
     2101 gggcaccgag acgtcgaagc gcaccggtgg cggggttcct tccagaacat gcagcttgcc
     2161 agccatgcga tggctcaaac gtgcgtcttc gcccgcggct aggaccgcga cccacgcatt
     2221 cccgtagagc gacggcgcga ctccggccag acaatagatt gccgcgtgac accgcagcca
     2281 ggtgccggtc gccactaagt ggcggatgcg cttgtccgag tactgcgccg caagtgcttg
     2341 cgcacgagtg aaaacaccac attgggcggc tgcgatggcg acaaggagag gttccatctc
     2401 gacaatgtcg gcactgcgac gtagtcacga ccgatgtgcg gtccgattgt ggatggccgc
     2461 agccctgtgg atcgtcgacc cacccacagc tggtgcgttc acctacccca cagggcagaa
     2521 attgcacccg ctgcggcaat cgatgaccta gcgcaggtcg ccggtgacca tgccttcgtc
     2581 aacgggcggg acggcgatca gtcccagttc tcccgcagtg accagcagtg catcggaggg
     2641 aagaacccgc acgctgtagc cgaagggccc ggtgcggtcg agcgtcatct cgccctcgta
     2701 aacccaactg ccgtcgtcgc gcttgtcaga cagccgtagt gacccgtacg tcgcgccggt
     2761 gatctggtcg tgctcgtcga cccggccata tgcaacctgg acgtcgatgt catcggcagc
     2821 aagatcaccc agggcgacaa cggcccgcaa tgacacgacg gtgccgtact cgagggcatc
     2881 ggagacaccg cgcgcctcga tgtgctcgac gtgcacgccg ggccacgcag tcgtgacgcg
     2941 cttcttccag gccgcgaggt gtttcgcacc accgaacttc gccgattcca tcgcccgcga
     3001 cgactgcgcc gccggggtgt agaggttcgt gacgtaatcg cgcagcatgc gggtcgctac
     3061 gaccttcggg ccaagcgatg acaaggtgtg ccgcaccatc tcaagccagc gccgcggcag
     3121 accgtcgccg tcgtggtcgt aaaagcgcgg caccatctgc gtctcgacga tgtcatagaa
     3181 ggccccggcc tcgatctcgt cgcgacgctc ctcgggcagg tcgtcggccg acgggatcgc
     3241 ccacccgttg tcgccgtcga accactcgtc ccaccagccg tcgcgaatcg acaggttgag
     3301 cacgccgttc aacgcagcct tcatgcccga cgtgccgcac gcctcgagcg gccgcagcgg
     3361 gttgttcagc cagacgtcgg cgccggcgca cagcacgcgc gccaatgcca tgtcgtagtc
     3421 gggcaggaac acgaccctgt gccgcacccc cgcgccgtcg gcgaatttca cgacctcttg
     3481 gatcagccgc ttgccaccgt cgtcggccgg atgcgctttg cccgcgatca cgaactgcac
     3541 cggcctgtct cggtcgagca gcagcgcagt gaaccgcttc gggtcttgca gcatcaacgt
     3601 gagccgcttg taggaaggca cccggcgcgc gaaaccgatg gtgagcacgt cggggtcgag
     3661 cacgtcgttg atccagccga gctctgactc catggcccc
//
LOCUS       DASWVF010000296         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_237507, whole genome shotgun sequence.
ACCESSION   DASWVF010000296 DASWVF010000000
VERSION     DASWVF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237507"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VGD55_05240"
     CDS             complement(<1..1470)
                     /locus_tag="VGD55_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0869778.1"
                     /translation="MLVRRRGIQIAAGVAVVALTLAACSSSSKKQAAASSSPATTGSA
                     SSSGSAPVASASAPVSSAPAGTGDLAPENLNNSGTPVVGGTLHMLGVGDVDYMDPNIS
                     YYSAGYEALRLWSRQLVTFPAVAGKATTDVADLATQIPTTANGGISADGLTYKFTITK
                     GDMWNTTPARQVTAADEVRGVKRTCNPAQPFGGEADFESLIVGLQAFCDGFAKVDPKS
                     PTAIAAYQNATALAGVSVDASDPDTVDFKLTTPASYFIAMLSLPAFSPSPAEYDAYLP
                     ASPELAAHTLSDGPYMVTSYTATKEIDFSRNPAWSASTDNVRKAYVDKVVINETGDQT
                     AIQQQLQANTAGADMEWDTFPPVSAVQGLIDAKDPNFYLGPTFSTNPYIIFNFDSPNN
                     GGALGKVAVRKALEEAISRANLIQDDNGPAISPPLTHVLPAGISGTTSNTSIDLYPYD
                     AAKAKADLAAAGYPNGLTVKFLYRPKSSLSSKIFTTLQQD"
     gene            complement(1678..2463)
                     /gene="tpiA"
                     /locus_tag="VGD55_05245"
     CDS             complement(1678..2463)
                     /gene="tpiA"
                     /locus_tag="VGD55_05245"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0869779.1"
                     /translation="MAARGSSRKPLIAGNWKMNLNHLEAIGFVQKLAFSLSDHDYDEV
                     DVAVLPPFIDLRSVQTLVDGDRLRLLYGAQDVSAHASGAYTGEISASMLAKLGCTFVV
                     VGHSERRQYHHEDDALVNAKVKATLAAGMTPILCIGEPLHVRQAGDHVDHTLSQLDGG
                     LADIPADADVVIAYEPIWAIGTGEVATPDDAQQQCAAIRKRIGELYDVAVGAKTRILY
                     GGSVKGNNATAIIAERDVDGALVGGASLDSDDFVRIVRFNNTN"
     gene            complement(2466..>3162)
                     /gene="pgk"
                     /locus_tag="VGD55_05250"
     CDS             complement(2466..>3162)
                     /gene="pgk"
                     /locus_tag="VGD55_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0869780.1"
                     /translation="RPHAAGGLVLAEVSVLQRLTDDLHRPYVVVLGGAKVSDKLGVIS
                     NLLAKVDRLLIGGGMAYTFLAAQGYEVGNSLLEADQLDVVRGFLAEADKRFVDLVLPV
                     DIVAATHFSADAEHVVVPADQIPADREGLDIGPQTRALFASKLADARTVFWNGPMGVF
                     EFEAFAAGTKALAMALVGVDGLTVVGGGDSAAAVRKLGFEESDFGHVSTGGGASLEYL
                     EGKTLPGLQVLED"
BASE COUNT          550 a          981 c         1044 g          587 t
ORIGIN      
        1 atcctgctga agggtggtga aaatcttgga cgacaacgaa gacttcggcc ggtacaagaa
       61 cttcaccgtc aggccgttcg ggtaaccagc ggctgcaaga tccgccttcg ccttcgcggc
      121 atcgtatggg tagaggtcga tgctcgtgtt actcgtcgtc cccgaaatgc ctgcaggcaa
      181 gacgtgggtc aacggcgggg aaatggccgg gccgttgtcg tcctggatca ggttggcgcg
      241 gctgatcgct tcctcgagtg ccttgcgaac agcgaccttt ccaagggcgc cgccgttgtt
      301 cggcgaatcg aagttgaaaa tgatgtacgg gttcgtcgag aacgtcggac ccaggtagaa
      361 gttcgggtcc ttcgcgtcaa tcaacccttg gaccgcagac accggcggga aggtgtccca
      421 ttccatgtcg gcgcctgccg tgttggcctg cagttgctgc tggattgccg tctggtcacc
      481 agtctcattg atgacaacct tgtcgacgta tgccttgcgc acattgtcgg tgctggcact
      541 ccacgctggg ttgcgcgaga agtcgatctc cttggtggcg gtgtacgacg tgaccatgta
      601 cgggccgtca gagagtgtgt gcgccgcgag ttccgggctc gccgggaggt acgcgtcgta
      661 ctcggccggt gacggcgaga acgcgggcag tgaaagcatc gcaatgaagt acgacgcagg
      721 tgtcgtcagc ttgaagtcca ccgtgtcggg gtcactggcg tctaccgaga cgcccgccag
      781 cgcagtggcg ttttggtaag cagcgatcgc ggtaggtgac ttcgggtcga ccttggcgaa
      841 accatcgcag aacgcctgga ggccaacgat cagcgactcg aagtcagctt cgccgccgaa
      901 cggctgcgcg gggttgcagg tgcgcttcac gccacgcacc tcgtcagctg cggtcacttg
      961 ccgcgccggc gtcgtgttcc acatgtcgcc cttggtgatg gtgaacttgt aggtcagtcc
     1021 gtccgcgctg atgccaccat tcgcggtggt cgggatctgc gttgcgaggt cggcgacgtc
     1081 ggtcgtcgcc tttcctgcga cagccgggaa ggtcaccagc tgacggctcc acagccgcag
     1141 cgcctcatag ccggcggagt aataggagat gttcgggtcc atgtagtcga cgtcaccgac
     1201 gccgagcatg tggagtgttc cgccgaccac cggagtgccg ctgttgttca ggttctcggg
     1261 agcgaggtcg ccggtgcctg ccggagcgct gctaacgggc gcgctcgccg atgcgaccgg
     1321 ggcgctacca ctggaactgg cactgcccgt tgtggctggg ctggaactgg cggccgcttg
     1381 cttcttgctg ctgctactgc acgcggcaag ggtaagtgcc acgaccgcaa cgcctgctgc
     1441 gatctgaatg cctctacgtc tgacgagcat gtctctcctt ctggcgacgc accttgtggg
     1501 tggtccgcga gtagtagctc ccgaacgccg ggtttttagg cgaacgcccc ccgcgcggtc
     1561 gcggcgactg tagccaccgc atgcccggtc ggacgatcct tgggccgaac ttaacgcaaa
     1621 tcgtgacaca agagttacgt actgggtccc tacagacgag ttgtcgccga agtgacacta
     1681 gttcgtgttg ttaaagcgaa caattcggac gaaatcgtct gaatcgaggc ttgcacctcc
     1741 taccagagcg ccatcgacgt cacgctccgc gatgatcgcg gttgcgttat ttcccttgac
     1801 ggacccgcca taaagtattc gcgttttggc ccctacggca acgtcgtaca actccccgat
     1861 ccgcttgcgg atcgcggcgc actgctgctg ggcgtcgtca ggcgtcgcca cctcaccggt
     1921 accgatcgcc caaatgggtt cgtaggcgat cacgacatcg gcgtcggcgg ggatatcagc
     1981 caatccgccg tcgagttgag acagcgtgtg gtcgacgtgg tcgccggcct ggcgcacgtg
     2041 gagtggctca ccgatgcaca agatgggcgt catcccggcg gcaagggtgg ctttgacctt
     2101 ggcgttcacg agtgcgtcgt cctcgtggtg gtactgccgg cgctcggagt gaccgacgac
     2161 cacgaaggtg cagccgagct tggccagcat cgacgcagag atctcgcccg tataggcgcc
     2221 ggacgcgtgc gccgagacgt cctgcgcgcc gtagaggagc cgcagccggt cgccatcgac
     2281 gagagtctgc acgctccgca ggtcgatgaa cggcgggagc accgcgacgt cgacctcgtc
     2341 gtagtcgtgg tctgagagcg agaaggcgag cttctgcacg aagccgatcg cctcgaggtg
     2401 gttgaggttc atcttccagt tgccggcgat caacggctta cgactcgagc cccgggctgc
     2461 caccctcagt cctccagaac ctgaaggcca ggcagcgtct tgccctcgag gtactcaagg
     2521 ctggcgccgc caccggtcga gacatggccg aaatcgctct cctcgaaccc aagtttgcgc
     2581 accgccgcgg ccgagtcgcc accgccgacg accgtcaacc cgtcgacgcc gaccagcgcc
     2641 atggcgagtg ccttggtgcc cgccgcgaat gcctcgaact cgaagacccc catcgggccg
     2701 ttccagaaga cggtacgcgc gtcggcgagc ttcgatgcaa acaaggcacg tgtctgcggg
     2761 ccgatgtcga ggccttcgcg atcggccgga atctggtcgg ccgggacgac gacgtgctcg
     2821 gcatcagcgg aaaagtgagt cgcggccacg atgtcgacag gcagcaccag atcgacgaag
     2881 cgcttgtcgg cctcggcgag gaagccgcgg acgacatcga gctgatctgc ctcgaggagt
     2941 gagttgccga cctcgtagcc ctgcgccgcc agaaacgtgt aggccatgcc gccgccgatg
     3001 agcagccggt cgaccttggc gagcaggttg gagatgacgc cgagcttgtc ggagaccttc
     3061 gcgccgccga gcacgaccac gtaggggcgg tgcaggtcgt cggtgaggcg ctggagcacc
     3121 gacacctcgg cgagcaccag tccgcccgcc gcgtgcggtc gc
//
LOCUS       DASWVF010000297         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_492173, whole genome shotgun sequence.
ACCESSION   DASWVF010000297 DASWVF010000000
VERSION     DASWVF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492173"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2484)
                     /locus_tag="VGD55_05255"
     CDS             complement(<1..>2484)
                     /locus_tag="VGD55_05255"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869781.1"
                     /translation="GGIRAAVAQSAERLYDSLPTTDRVALRIVMLRLVTPTPSGDPVG
                     TRVPARVFAGDGETARLLDVLVRARLVTTDAETVAIAHESLTRVWPRLRSWLDEDIDG
                     LRILQHLQVAADGWRAVGRPEEELYRGARLQAALEWRERGRPVLADAEIEFLAASETR
                     EDDERRHQDSALRQQIRRNRQLRAALTAVIGLLVLALVAGTVAAVSSRHARAATAKAD
                     AARLGAAALVQPQADTALLLARQAVALAATPTTEGGLLEALGREPGLVRVDRPGAVQF
                     NGYPFESDDWVSADGTRVLVNGGNGIDLIDTSTGHFVGGGPLEPQLTDAGTGPLPVGF
                     IDGGRTALVAVETSSAVPSRSIQAFNAADGHPIGTPQLVPNSVSGYVSEMDRLRISPD
                     GRTLVSILDRAVRMWRRSTTTSAWGPLIQFALPSLPPSVPNGDLLLSATFSTDSSRVA
                     FLLALTGPPFGQPPNVGIVVDTDTEQVLLPLVIPQAHATPEVFAISPAGDRLAIGYQD
                     GSVELRSTSDANAVVRIPGTSQATALGWTADGARLAVGHDDGGLDVFNTNPLQAVVSY
                     PAGGQEVQVAALRGDRLISQDFSQAITVRSLANTSAFMRETPVEPANSIAAGPSGTVV
                     AVGDAAGNVALYSQKTLGALLHLSLGPYTSPDTTANPDFHERVSALAITPDGSALIAA
                     DRVGHLRMWSLPDGRLLWSRDDVPASALAISPNGRYLATSGFTQEPTDENPDSYPIST
                     SLTVWDLATKLPVLTYNLPGVTTNDYSTPKPRAVLFSPDSKLLAAAYFEETLVVTNFA
                     GPARLVVITPQSAPNVSSIVFSPDGKELFV"
BASE COUNT          439 a          789 c          832 g          424 t
ORIGIN      
        1 aacgaacagt tctttcccgt ccgggctgaa gacaatggac gagacgttcg gagccgactg
       61 cggcgtgatg accacgagtc gagcaggccc cgcaaagttc gtgacgacca atgtctcctc
      121 aaagtacgca gccgcgagca gtttcgagtc gggtgagaaa agcacggccc taggcttcgg
      181 tgtcgagtaa tcgttcgttg tcacgcccgg gaggttgtag gtaagcaccg gcagttttgt
      241 cgccaggtcc cacacggtca ggctggtcga tatcggatac gagtccgggt tctcgtcagt
      301 cggttcctgt gtgaaccccg aggttgcgag gtagcgaccg ttcggcgaga tcgcgagggc
      361 tgacgccggc acgtcgtcgc gtgaccacaa caggcggcca tccgggagtg accacatccg
      421 caggtggccg acgcgatcag ctgcgatcag ggcggatccg tcgggggtaa ttgccaacgc
      481 gctgacgcgt tcgtgaaaat cagggttcgc agtggtgtcc gggctcgtgt aagggccgag
      541 cgacaagtgc agcagcgcgc cgagggtctt ctgcgaatac agggcgacgt tcccagccgc
      601 atcgccgacg gccaccaccg tgccggacgg tccggccgcg atcgagttgg ctggttctac
      661 cggtgtttcc cgcatgaatg cgctggtgtt cgcaagtgat cgcacggtga tggcctggga
      721 aaagtcctgg ctgatcagcc gatcaccacg tagtgcagcg acctgcacct cctgcccgcc
      781 cgcggggtag ctgacgacgg cctgaagcgg gttggtgttg aacacatcga gaccgccgtc
      841 gtcgtgtccc acagccagcc gggcgccatc cgccgtccag ccgagcgctg ttgcctgaga
      901 cgtgccaggg atgcggacga ccgcgttagc atcggacgtc gagcgcaact cgaccgagcc
      961 gtcttggtaa ccgatggcaa gccggtcgcc ggcgggcgag atcgcgaaca cctcgggtgt
     1021 ggcatgtgcc tgcgggatca ccaacggcaa caacacctgc tccgtgtcag tatcaaccac
     1081 gatgccgaca ttcggtggtt gcccgaatgg tggtcccgtc agcgccagca ggaacgccac
     1141 ccgtgaactg tcagtgctga atgtcgccga gagcaagagg tctccattcg gtaccgacgg
     1201 tggcagggag ggcaaggcaa actggatcag cggaccccag gcgctggtgg ttgtgcttcg
     1261 tctccacatg cgcacagccc tgtcgaggat cgacacgagc gtccggccgt ccggtgagat
     1321 acggagcctg tccatctcgg agacgtaccc cgacaccgaa ttgggcacga gttgtggtgt
     1381 accgatcggg tggccgtccg cggcgttgaa tgcctgaatc gagcggctgg gtacagcgga
     1441 cgatgtctcg acagcgacga gggcagtacg cccaccgtca atgaagccca cgggaagcgg
     1501 tccagtgccc gcgtctgtga gttgcggctc cagcgggccg ccgccgacga agtgacctgt
     1561 gctggtgtcg atcaggtcga ttccgttgcc gccgttcacg agcacgcgag tgccgtcggc
     1621 gctgacccaa tcgtcgctct cgaagggata tccgttgaac tgcactgcgc cgggacggtc
     1681 tacacggacg agaccgggtt cacgaccgag ggcctcgagc aggcccccct cggtggtcgg
     1741 tgtagcggcg agcgccactg cctgccgggc gagcaagagg gccgtgtcgg cctgcggctg
     1801 cacaagggcg gcggcaccca accgcgctgc atccgccttt gcggtggccg ctcgcgcatg
     1861 acgactgctc accgctgcaa ctgtgccggc tacgagagcg agtaccagga ggccgatgac
     1921 ggccgtcagt gctgcccgca gttgtcggtt gcgacgtatt tgctgccgaa gcgcgctgtc
     1981 ctggtgtcgt cgctcgtcat cttcgcgtgt ctcggatgcc gcaaggaact cgatctcggc
     2041 gtcggcgagc accggtcggc cacgttcacg ccactcaagc gccgcttgca atctcgcgcc
     2101 gcggtagagc tcttcttccg gtcgcccgac agctctccat ccgtctgcgg cgacctgcaa
     2161 atgctgcaag atgcgcagcc cgtcgatatc ctcgtcgagc cacgaccgca ggcgcggcca
     2221 cacgcgagtc agcgactcgt gtgcaatggc gacggtctct gcgtccgttg tgacgagtcg
     2281 agcccgcacc aggacgtcga gcagccgcgc cgtctcacca tcaccggcaa acacccgagc
     2341 cggcacgcgg gtgccgacag ggtcgccgct cggagtcgga gtcaccaatc tgagcatcac
     2401 gatgcgcaag gcgacgcggt ccgtcgtcgg aagggagtcg tagagccgct ctgcggactg
     2461 cgcaaccgct gcgcggatac cgcc
//
LOCUS       DASWVF010000298         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_8272, whole genome shotgun sequence.
ACCESSION   DASWVF010000298 DASWVF010000000
VERSION     DASWVF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8272"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="dhaK"
                     /locus_tag="VGD55_05260"
     CDS             complement(<1..997)
                     /gene="dhaK"
                     /locus_tag="VGD55_05260"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY0869782.1"
                     /translation="MKKLINDPADVVKEALLGIAAAHPELKVDHANKIIYRGDAPVNG
                     KVGLISGGGSGHEPLHGGFVGLGMLDAACAGEIFTSPVPDQMLEATKRVDGGAGVLHI
                     VKNYTGDVMNFEMAAEMAAAETGLDVIAVVTDDDVAVQDSTWTAGRRGVGVTVLLEKI
                     AGAAAEQGQSLKEVADVARHVNEHGRSMGMALTSCIVPAAGKPTFDLADNEMEIGVGI
                     HGEPGRRRVAVAPAKEVAAMLVEPILADMPLGRGDSVIAFVNGMGGTPLIELYVMYNE
                     VAKILQGQGVTIARSLVGPYITSLEMAGCSVTLLKTDDATIRLWDAPVNTPGLRWG"
     gene            complement(1048..>2211)
                     /locus_tag="VGD55_05265"
     CDS             complement(1048..>2211)
                     /locus_tag="VGD55_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0869783.1"
                     /translation="EQIVGAKVMDVETDKEFVIRAKQVINATGVWTDDTQEMAGGRGR
                     FHVRASKGVHLVVPRNRIQLDTGLILRTEKSVLFVIPWGRHWIIGTTDTDWELDKVHP
                     AASSTDIKYILDHVNAVLRTPLGPDDVEGVYAGLRPLLAGESEQTSKLSREHSVGQPV
                     PGLTVIAGGKYTTYRVMAKDAVDVARRGMSASVPDSVTERLALLGADGYEAMWNKRNA
                     LAASSGLHVTRIEHLLHRYGSLTTDLLELVAQDASLGEPVSSGSDYLRAEIVYATSHE
                     GALHLDDVLTRRTRISIEEWDRGSGSARDVAQLMARVLEWDDETIEREVDHYLLRVEA
                     ERASQRQPDDQTADAARLGAPDIVPLRGDPREQDDVAPTRLGKLKSIKSAEAN"
BASE COUNT          341 a          707 c          707 g          456 t
ORIGIN      
        1 ttccccaccg aagacccggc gtgttcaccg gagcgtccca gagccggatg gtggcgtcgt
       61 cggtcttgag caaggtgacc gaacaacccg ccatctcaag cgacgtgatg taagggccga
      121 cgagcgagcg ggcgatcgtg acgccttgcc cctgcaggat cttggcgact tcgttgtaca
      181 tcacgtagag ctcgatgagc ggcgtgccgc ccatgccgtt gacgaacgca ataaccgaat
      241 ctcctcggcc caatggcatg tccgcgagga tcggctcgac caacatcgcc gcgacctcct
      301 ttgcgggcgc gaccgctacc cggcgccgac ccggctcgcc gtggataccg acgccgatct
      361 ccatctcgtt gtcggcgagg tcgaaggtcg gcttgcctgc agccggcact atgcacgacg
      421 tgagcgccat gcccatgctg cgcccgtgtt cattgacgtg gcgggcgacg tccgcgactt
      481 ccttcaggct ctgtccctgt tcggcggccg cgccagcgat cttctcgagc agaacggtga
      541 cgccgacccc tcggcgtccg gccgtccacg tgctgtcttg aacggcgaca tcgtcatcgg
      601 tcacgacagc gatgacgtcg agtccggtct ctgctgcggc catctcggcg gccatctcga
      661 agttcatgac gtcgccggtg taattcttca cgatgtgcag gaccccagcg ccaccgtcga
      721 ctcgcttggt cgcctcgagc atctggtcgg gtacgggcga ggtgaagatc tcgcccgcgc
      781 atgcggcgtc gagcattccg aggccgacga agccgccgtg caacggctcg tgaccggagc
      841 cgccgcccga gatgagtccg actttgccat tcacgggtgc gtcgccgcgg tagatgatct
      901 tgttggcgtg gtcaaccttc agctccggat gcgcggccgc gattccaagg agtgcttctt
      961 tgacgacgtc cgctggatcg ttgatgagct tcttcattga acgctcctgt tcttgatcgt
     1021 ggttgcggtt gggtggacgg caggggtcta gttggcttcc gctgacttga tcgacttcag
     1081 ctttcccaac cgggttggcg ctacgtcgtc ttgttcgcgg gggtcgccgc gcagcgggac
     1141 gatgtcaggg gcaccgagcc gcgcggcgtc cgcggtttgg tcgtcgggtt ggcgctgcga
     1201 agcacgctcg gcctcgacgc gcagcaggta gtggtcgacc tcgcgttcga tggtttcgtc
     1261 atcccactcg agtacgcggg ccatcagctg cgcgacgtcg cgtgccgaac cgctaccccg
     1321 atcccactcc tcgatggaga tcctggtccg ccgtgtcagc acatcgtcga ggtgtagcgc
     1381 gccttcatgg ctagtcgcgt acacgatttc ggcgcgcaga tagtcgctgc ccgatgagac
     1441 cggttcaccg agacttgcat cctgcgcgac aagttcgagt agatcggtgg tgagtgaccc
     1501 gtagcggtgg agcagatgct ctatccgtgt gacatggagt ccggacgacg ctgcgagtgc
     1561 gtttcgcttg ttccacatgg cttcgtagcc gtcggcgccg agtaacgcca gccgttcggt
     1621 gacggagtcg ggcacgctcg cactcatgcc acgacgggcg acatcgacgg cgtccttcgc
     1681 catcacccgg tacgtcgtgt actttccgcc cgcgatgacg gtcaagcccg gcactggttg
     1741 gccgaccgag tgttcgcgcg aaagcttgct cgtctgctcg gattcgccgg ccagcaatgg
     1801 acgcagccct gcgtagacac cttcgacgtc atcgggcccg agcggcgttc ggagcacggc
     1861 gttaacgtgg tcgaggatgt acttgatgtc agtactgctt gccgcggggt gaactttgtc
     1921 cagctcccag tcggtgtcgg tggtgccgat aatccagtgc cggccccacg gaatgacgaa
     1981 gagcacgctc ttctctgtgc gcaggatcag tccggtgtcg agctgaatgc gattacgcgg
     2041 gacgacgagg tgcacgcctt tcgatgctct gacgtggaac cggccgcgcc caccggccat
     2101 ttcctgcgtg tcgtcggtcc acactccggt ggcgttgatg acctgcttgg cccggatgac
     2161 gaactccttg tccgtctcga cgtccatgac tttcgcgccg acgatttgct c
//
LOCUS       DASWVF010000299         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_313782, whole genome shotgun sequence.
ACCESSION   DASWVF010000299 DASWVF010000000
VERSION     DASWVF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313782"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="glgX"
                     /locus_tag="VGD55_05270"
     CDS             <1..1388
                     /gene="glgX"
                     /locus_tag="VGD55_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0869784.1"
                     /translation="GEQVQEFKALVRTLHEADIEVILDVVYNHTAEGNHLGPTLSFRG
                     LDNAAYYRLSEDDQSKYMDYTGTGNSLNARNPHALQLIMDSLRYWVLEMHVDGFRFDL
                     ASVLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWDVGAGGYQVGNFPPLWSEWNG
                     KYRDTVRDFWRGEPATLGEFASRLTGSSDLYANSGRRPMASINFVTAHDGFTLQDLVS
                     YNEKHNEANGEGGKDGTDDNRSWNCGAEGPTDDVAIMTLREQQKRNFLTTLFLSQGVP
                     MLLHGDEFGRTQGGNNNGYCQDSEISWVDWSLRTEHEVQLDFARMLSALRRDHPVFRR
                     RRFFDGHPVRAEGEDLRDIAWLTPGGAEMSAADWATGYAKSVGVFLNGDAIGYPDQHG
                     RAVVDDSFLLLFNAADTGIDFITPSAEYGERWEIIIDTTKPLDVEQPSIKAETAVHVE
                     GRCVLVLRRVV"
     gene            1378..>2176
                     /locus_tag="VGD55_05275"
     CDS             1378..>2176
                     /locus_tag="VGD55_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0869785.1"
                     /translation="MSSSPPGSTYRLQLGPQLTFAQAAKLAGYLADLGVTHAYLSPIL
                     TAGKGSTHGYDVVDHSRIDADLGGARGFAALGRAFQKAGIGIIVDVVPNHMAIPLPEY
                     QNGALWSVLREGRGSAFAPWFDIDWDAGGNRILMPVLDGPLEENLDRLKLVERAGALE
                     LAYFDHRFPLADATATLPIPELVEAQHYRLADWRSASTELNYRRFCDITSLIGLRVED
                     PAVFEASHTVLLDQLARGRIDGLRIDHPDGLADPAGYLDDLSGTTDTA"
BASE COUNT          409 a          714 c          654 g          399 t
ORIGIN      
        1 gcggcgagca ggtgcaggag ttcaaggccc tcgtgcgcac ccttcacgag gcggatatcg
       61 aggtgattct cgacgtcgtc tacaaccaca ctgccgaggg caatcacctg ggccccacat
      121 tgtcgtttcg ggggctcgac aacgcggcct actaccggct ctcagaagac gaccagagca
      181 agtacatgga ttacaccggt accggcaaca gtctgaatgc tcgcaaccca cacgcgctac
      241 agctcatcat ggactcgttg cgctactggg tactcgaaat gcacgtcgac ggatttcgtt
      301 tcgacctggc atcggtgctg gctcgcgagt tctatgacgt cgaccgactg tctgcattct
      361 tcgacctggt gcaacaagac cccgtggtaa gccaggtcaa actcatcgcc gaaccgtggg
      421 acgtcggcgc gggcgggtac caggtcggca acttcccgcc actgtggagc gagtggaacg
      481 gcaagtatcg cgacaccgtg cgcgactttt ggcgcggcga gccggcaacc ctcggcgagt
      541 tcgcgtcgcg gctcaccgga tcgagtgatc tctacgccaa ttccggacgt cgacccatgg
      601 cgtcgatcaa tttcgtcacc gcccacgatg gtttcacgct gcaagacctg gtgtcctaca
      661 acgagaagca caacgaggcc aatggtgaag gcggcaagga cggcactgac gacaaccggt
      721 catggaattg cggcgccgaa gggccaaccg acgacgtcgc gatcatgacg ctccgcgagc
      781 agcaaaagcg caactttctg acgacgctgt tcctgtcgca gggtgtgcct atgctgttgc
      841 acggcgacga gttcggtcga actcaaggtg gcaacaacaa cggatactgc caggacagtg
      901 agatttcgtg ggtcgactgg tcactacgca ctgagcacga ggtgcaactc gatttcgctc
      961 gcatgctgtc tgcgttgcgt cgcgaccacc cggtgtttcg ccgccgccga ttcttcgacg
     1021 gccacccggt tcgagccgag ggtgaggatc tgcgcgatat cgcctggcta acgccgggcg
     1081 gtgccgaaat gtcggcggcc gactgggcta ccggatatgc gaaatcggtc ggggttttcc
     1141 tgaacggcga cgcgatcggc taccccgatc agcacggtcg cgcagtcgtc gacgattcat
     1201 ttcttctgct cttcaatgcc gccgacaccg gtatcgattt catcactccg agcgcggagt
     1261 acggcgagcg ttgggaaatc ataatcgaca cgaccaaacc tctcgacgtc gagcaaccat
     1321 cgatcaaagc cgagaccgcg gttcacgtcg agggccgttg cgtgctggtg ctgcgccgtg
     1381 tcgtctagcc cgccggggtc gacgtaccgg ctccagctcg gcccgcagtt gacgttcgcg
     1441 caagccgcga agctcgccgg ctatctggct gacctcggcg tcacccacgc ctacctatct
     1501 ccgatcctta ccgcggggaa agggtcgacc cacggctacg acgtcgtcga ccacagccgg
     1561 atcgacgccg acctcggcgg cgcccgagga ttcgccgcat tgggtcgcgc tttccagaag
     1621 gcgggcatcg gcatcatcgt cgacgtcgtg cccaaccaca tggcgatccc gctgcccgaa
     1681 taccaaaacg gcgcactctg gtcggtgctg cgtgagggtc gcgggtctgc gtttgctccg
     1741 tggttcgaca tcgactggga cgccgggggc aaccggattc tgatgccggt actcgacggg
     1801 cctctcgagg agaacctcga tcgcctaaaa ctcgtcgaac gtgcgggcgc cctcgagttg
     1861 gcgtacttcg accatcggtt cccactcgcg gacgccacgg caacgcttcc gattcccgag
     1921 ctcgtcgaag cccagcacta tcgcctcgcc gattggcgat cagcgtcgac cgaactgaac
     1981 taccggcgct tctgcgacat caccagcctg atcggcctgc gcgtcgagga tcccgccgtg
     2041 ttcgaggcga gccacaccgt gttgctcgac cagcttgcac gcggccgcat tgacgggctg
     2101 cgcatcgatc accccgacgg tctcgccgac cccgccggct atctcgacga cctttccggc
     2161 acgaccgaca ccgcat
//
LOCUS       DASWVF010000300         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_339472, whole genome shotgun sequence.
ACCESSION   DASWVF010000300 DASWVF010000000
VERSION     DASWVF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339472"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VGD55_05280"
     CDS             <1..597
                     /locus_tag="VGD55_05280"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEY0869786.1"
                     /translation="ERGVVVGAHIGYPDLQGFGRRPMVLAPDELTAGVIYQLGALDAI
                     VRSLGSQVRFVKPHGALYHKAAVDQGVARAVVAGVRAFDPTLPIVTLPNSAVVAQTAA
                     VGMAAVSECFADRAYTSAGGLVSRSLPGAVLHDPAVVAARALAMATDGLVEAIDGSRV
                     RVRADSMCVHGDTAGAAALAATIRAALEQAGVELKAFA"
     gene            594..1226
                     /locus_tag="VGD55_05285"
     CDS             594..1226
                     /locus_tag="VGD55_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEY0869787.1"
                     /translation="MTFAVRPVGDAAFLVDVDPGQRHAVARAIAERGLAGVIDVVPAE
                     RSVLVTFDPRTFDATGADAEVLADLASSAIYDGLREASAMAAAVEIEVRYDGADLDDV
                     AALTGLSTRDVVARHLAGDYVVAFMGFAPGFGYLDGLDAALHVPRLATPRTRVDAGAV
                     AIAGARSCIYPGASPGGWRVVGHTRDVLFDASADMPSLLQVGVRVRFREA"
     gene            1226..2188
                     /locus_tag="VGD55_05290"
     CDS             1226..2188
                     /locus_tag="VGD55_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEY0869788.1"
                     /translation="MEAPTSILVEKPGLRTTMQDAGRAGWAHLGVPPCGAVDALSRRL
                     GNALVGNHDDAAVLETTLSGPALRCGADAVVAVTGAPCPLLIDDEPVAFGVAVAVRAG
                     QLLNIGRAAVGLHTYVAVAGGFAAPRVLGSRSTDTLSGLGQPPLAAGDVLETDVATEG
                     DVIERPADSALLRFVLDGGRRPVRLLVGPGVDWLTKDAAEVLYSSEFVVSSASDRVGV
                     RLAGPALGRSREGELDSQGMVNGAVQLPPDGAPIVLLADRAVTGGYPVVGVVATIDLP
                     LVAQARPGTAIRFVATSVEDARAALAAQEMELLRWRRGHTAPVR"
     gene            complement(2151..2489)
                     /locus_tag="VGD55_05295"
     CDS             complement(2151..2489)
                     /locus_tag="VGD55_05295"
                     /inference="COORDINATES: protein motif:HMM:NF002377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HEY0869789.1"
                     /translation="MPFDLSMWKLMVLGVIALVVFGPDKLPQLARDAGRALRQVRMMA
                     QTARTELKAELGDTVGEFDFSDLNPRTFVRKHLLDDLDTNLTAPSAAARPAAAQGPLN
                     APAPYDLDAT"
     gene            complement(2577..2918)
                     /locus_tag="VGD55_05300"
     CDS             complement(2577..2918)
                     /locus_tag="VGD55_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEY0869790.1"
                     /translation="MAQRISVNLVDDIDGGSAVETIHFALDGVDYEIDLGRRNAERLR
                     GAMTGHVEAGRRLVKTTGKGRSRTVVQRRVEVAADPRAVRAWAKANRIAVPDRGRIPL
                     AVLERYRAAGH"
     gene            3146..3487
                     /locus_tag="VGD55_05305"
     CDS             3146..3487
                     /locus_tag="VGD55_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869791.1"
                     /translation="MSSVRGGGHRMGAGAIDRQPAASPTPDAGSLIPDAGERWRNALA
                     KLEYAERHEAPASEVEELANQVIEARVAMFQSGVQGGWALPAFLRSALDRDRELLEVA
                     PASRMQPHGRV"
     gene            3516..4367
                     /locus_tag="VGD55_05310"
     CDS             3516..4367
                     /locus_tag="VGD55_05310"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0869792.1"
                     /translation="MTEAVQFTDPVAFHAEGPVWAPAWGGLRYVDMFAGDVLAVHADG
                     EISRRHVGTIAAALRPRRSGGAVIAVERGFVLEGPAGELSALPELWADPTIRMNEGGC
                     DPDGRFYCGSMGYDQGEGRGSFYRLDADGTASLQFAGVTISNGLGWSPDGSIAYYTDT
                     KTRRIDVFDYAAEAGLTSRRPFVAIADGAGGPDGLTVDAEGCVWVALYGGSAVHRYRP
                     DGVLDGVVELPVTNVTACTFGGDDLDQLFITTSREHLADGVQPSAGSLFTCTPGVIGL
                     PVLPFAG"
     gene            4484..>5773
                     /locus_tag="VGD55_05315"
     CDS             4484..>5773
                     /locus_tag="VGD55_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0869793.1"
                     /translation="MRVTKWTAANVVLAALALTGGSLAYATVGTPAAATTKAATRTTT
                     VASGTVMTTVSSSGNVTAPNNVTVNFATGGTLTEIDVKVGDTVKQGQVLARVDSTAAK
                     ASYDSANAQLASAQANLAELQDGVTSLVKQSLQISNAQAAQQVVSAKTSLTNAQQNLG
                     FDQTNLAAAITKAQEQLADDQATAAQDSTTLQAALNQAESLSQTQITADDAQLGVDTS
                     TQTIDENQLATDQAKLAADHGTGPTLAADTAAVAADQAKLATDASKIASDNEKLVTDG
                     NSVTNAQNSLTTGELRDQQTLQKDQDAIGSAQTAQAQGIAKDNQAITNAQQQVTNAEL
                     SEQSTENNNAQKTAAPLSSTVQTDQAQVEQAQAQVATAKTTLDATALLASADGTVGAI
                     NDTVGQTVTAGTTSSASTSSSTSNSSSSSSGFMTLTNL"
BASE COUNT          948 a         1893 c         1960 g          972 t
ORIGIN      
        1 gagcgcggcg tcgttgtggg tgcccacatc ggctacccag atcttcaagg gttcggccgg
       61 cggcccatgg ttcttgcgcc cgacgagctc accgcaggcg tcatctatca actgggtgca
      121 ctcgacgcga tcgttcggtc actcgggtcg caggtgcgct tcgtgaagcc ccatggcgcg
      181 ctctaccaca aggcggccgt tgatcagggc gtcgctcgcg cggtcgtcgc aggggtgcgt
      241 gccttcgacc cgacgttgcc gatcgtgacg cttccgaact ccgccgtggt cgcgcagact
      301 gccgctgtcg gcatggcggc ggtgagtgag tgcttcgcgg atcgcgccta tacatcggcc
      361 ggtggcctgg tctcgcgctc gctgcctggc gcggttcttc acgatcccgc cgtcgttgcg
      421 gcgcgcgcgc tcgccatggc gaccgatggc ctcgtcgagg cgatcgacgg ttctcgggtg
      481 cgggtccgag ccgactccat gtgcgtgcat ggcgacactg ccggcgctgc agccctggcc
      541 gcgacgattc gagccgccct tgagcaggcg ggggtcgagc tgaaggcgtt cgcgtgacgt
      601 ttgcggtccg ccccgtcggt gacgctgcgt ttctggtcga cgtcgacccg gggcagcggc
      661 atgcggtcgc gcgcgccatc gccgaacgtg ggcttgccgg agtcatcgac gtcgttcccg
      721 cggagcgctc ggttctcgtc accttcgacc cacgaacctt cgacgcaaca ggggcggacg
      781 ccgaggtgct ggccgacctg gccagctcag ccatctacga cggcttacga gaagcgtcag
      841 ccatggcagc cgcagtcgag atcgaagttc gatatgacgg cgcggacctc gacgacgtcg
      901 ctgcgctgac cggactttcg acacgggacg tcgtcgctcg gcatctcgca ggtgactatg
      961 tcgttgcctt tatgggattc gcaccaggat tcggctacct cgacggtctc gatgccgcgc
     1021 tgcacgtgcc gcgactcgcg accccacgca cccgcgtcga tgccggagcg gtggcgatcg
     1081 ccggcgcgcg ttcgtgcatc taccccggcg cgtctccggg cggctggcgg gtcgtcggcc
     1141 acacgcgcga tgtgctgttc gacgccagcg ccgacatgcc ttcgctcttg caagtaggcg
     1201 tgcgtgtgcg cttccgcgag gcgtgatgga ggcgccgacc tcgattctgg tcgagaagcc
     1261 aggccttcgt acgacgatgc aggacgccgg ccgtgctggt tgggcacacc tcggcgtccc
     1321 gccgtgtggt gcagttgatg cgttgagccg ccggttgggg aacgcgctcg tcggcaatca
     1381 cgacgatgct gccgtgctcg agacgacgtt gtcagggccg gcgctgcgat gtggggccga
     1441 cgccgttgtc gcagtgacgg gcgcgccgtg ccctttgctc atcgacgacg aaccggtcgc
     1501 gttcggcgtc gccgtggcag tgcgtgcagg gcagctcctg aacatcggtc gggcagctgt
     1561 cggactgcat acctacgtgg cggtcgccgg tggattcgcg gcgccacggg tgctcggcag
     1621 ccgttcgacc gacacgctct caggtcttgg gcagcctccg cttgcagccg gtgacgtgct
     1681 cgagacagac gtcgcaaccg agggggatgt catcgagcgt cctgcagatt cagccctgct
     1741 tcgtttcgtg ctcgatggcg gacgccgacc agtgcggcta ctggttggtc caggcgtcga
     1801 ttggctaacg aaggacgcag cagaagtgct gtacagcagc gaattcgtgg tgtcttctgc
     1861 atccgaccga gtcggtgtgc ggttggcggg gccggcgctc ggcagatccc gcgagggcga
     1921 gcttgattcg caaggcatgg tcaacggcgc ggttcagttg ccgcccgacg gcgcgccgat
     1981 cgtgttgctc gccgatcgcg cggtgacggg tggctacccc gtggtcggcg tggtcgccac
     2041 gatcgacttg ccactggtcg cgcaggcgcg accgggcacc gcaattcggt tcgtggcaac
     2101 gtcggtcgag gacgcgcgag ccgcactggc cgcgcaggaa atggaactac tcaggtggcg
     2161 tcgaggtcat acggcgccgg tgcgttaagt ggtccttgag cggctgcggg tcgggccgct
     2221 gcggatggcg ctgtcaggtt ggtgtcgagg tcgtcgagca agtgcttgcg gacgaacgtg
     2281 cgcgggttca gatcggagaa gtcgaattcg ccgacggtgt caccaagttc ggctttcaac
     2341 tccgtgcggg cggtctgcgc catcatccga acctggcgca gcgcgcggcc ggcatcgcgg
     2401 gccagttgtg gcaacttgtc gggaccgaac acaacgagcg cgatcacgcc gagcaccatc
     2461 aacttccaca tgctcagatc gaacggcacg atcacacctc ctcggacagc agctaccgct
     2521 caggttaacc gagatcagat tcgttgcgac cagcgtttcg gatccccggg ccgggatcag
     2581 tgaccggcgg cgcggtaccg ctcgaggacc gcgaggggga tgcggccacg gtcaggcacg
     2641 gcgatcctgt tcgcctttgc ccacgcgcgg accgcgcgcg ggtcggctgc cacctcgaca
     2701 cggcgttgga cgacggtgcg actccggccc ttgccagtgg tcttcactaa tcggcgtccg
     2761 gcctcgacgt gacccgtcat cgccccgcgc agacgttcgg cattgcgcct accaagatcg
     2821 atttcgtagt cgacgccatc gagcgcgaaa tggattgtct ccactgcact tccgccgtcg
     2881 atgtcgtcta cgagattgac cgagatccgt tgcgccattg cctacccatc tccgttgagc
     2941 ccgttgtgaa gcgtcgttga agccggagtc catcatcgca gccccacatg gtgatcggca
     3001 gccacgggca ccgaccgcca gcctcgggca gtcaacgcgc attcggcggc ccacacgcgc
     3061 aatccggttg gtcgtctctg gacattcggt tgatgcatac ccaaatcgat tgaaggcaga
     3121 cttactcggg acataggggg acccggtgtc gagcgtgagg ggaggtgggc atcgcatggg
     3181 tgctggcgcg atcgatcggc aaccggctgc gtcgccgact ccggatgcgg gctcgctgat
     3241 tcccgacgcc ggcgagcgct ggcgcaatgc gctggcgaag ctcgagtacg cggagcggca
     3301 cgaggcgccg gcgagcgagg tcgaggagct cgccaaccag gtgatcgagg ccagggtcgc
     3361 gatgttccaa tccggtgtcc agggcggctg ggcgttgccg gccttcttgc gtagcgctct
     3421 cgaccgcgac cgcgagcttc tcgaagtcgc gccggcgtcc cgcatgcagc cccacggcag
     3481 ggtgtaggag acgcgcttcc ctacgctgtg tgctcgtgac cgaggctgtg cagttcaccg
     3541 accctgtggc gtttcatgcc gaaggccctg tgtgggcacc tgcgtgggga gggctgcgct
     3601 acgtcgacat gttcgccggc gacgtcttgg cggtgcatgc agacggtgag atcagccggc
     3661 gccacgtcgg caccatcgct gctgcattgc ggccgcgccg aagcggtgga gcagtcatcg
     3721 cggtcgaacg cggcttcgta ctcgagggcc cggcgggtga gctgagcgcc ctgcccgagc
     3781 tgtgggctga tcccacgatc cgcatgaacg agggcggttg cgacccggac ggccgcttct
     3841 actgcggatc catgggctac gaccaaggcg agggtcgcgg cagcttctat cgattggacg
     3901 ccgatgggac agcgtcgttg cagttcgccg gcgtcacgat ctcgaacggg ttgggctgga
     3961 gcccagacgg ctcgatcgcc tactacaccg acaccaagac gcgccgcatc gacgtgttcg
     4021 actacgcggc ggaggctggg ctgaccagtc gccgtccgtt cgtcgcgatc gccgacggcg
     4081 ccggcggccc cgatgggttg accgtcgacg ccgaagggtg cgtctgggtc gcgttgtacg
     4141 gcgggtcggc cgtgcacagg taccggcccg acggcgtcct cgacggagtt gtcgaactgc
     4201 ccgtgacgaa cgtgaccgcc tgcaccttcg gcggcgatga cctcgaccag ctcttcatca
     4261 cgacgtcgcg ggaacacctc gccgacggcg tccagccgag cgccggctcg ctgttcacct
     4321 gcacgcccgg agtcatcggc ctgcccgtgc tgccgttcgc cggctgagat tccacctcgc
     4381 cggagccatt ctcagcaaat tcgtggcgcc gtcacagcag acgtacagct tgacctgctg
     4441 gtctgtctgg gaggtgtgag aaccagccaa cgaagaggtg cgaatgcggg tcacgaagtg
     4501 gactgcggcc aatgtggtgc tggccgccct cgcgctcacc ggcggcagcc tggcctacgc
     4561 aaccgtcggg acgccggccg ccgcgacgac caaggcggcg acccgtacca ccacggtcgc
     4621 cagcggcacg gtcatgacga ccgtcagttc gagtggcaac gtaacggcgc ccaacaacgt
     4681 gacggtgaac ttcgcgaccg ggggcacctt gaccgagatc gacgtgaagg tcggcgacac
     4741 cgtcaagcag ggtcaggttc tcgcgcgcgt cgactcgacc gcggcgaagg ccagctatga
     4801 ctcggccaac gcgcagctgg cctccgcgca ggcgaacctt gcagagcttc aggatggcgt
     4861 gacgtcgctg gtgaagcaga gcctgcagat ctccaacgcc caggcagcgc aacaggtcgt
     4921 gtcggccaag acatctctga ctaacgcgca gcagaatctc ggcttcgatc agaccaacct
     4981 cgcggctgcc atcaccaagg cacaggagca actcgctgac gatcaagcca cggcggccca
     5041 agactcgacc acgttgcagg ccgctctgaa ccaggccgag tcgctttcgc aaacgcagat
     5101 cactgctgac gatgcacagc tcggcgtcga cacctctacc cagaccatag acgagaatca
     5161 gctcgcgacc gaccaggcca agctggcggc cgaccacggc accggtccga cactggcggc
     5221 cgacacggct gcggtggccg ccgaccaagc gaaacttgcg accgacgcga gcaagatcgc
     5281 gagcgacaac gagaagctcg tgaccgacgg caactcggtg acgaacgcgc agaactcgct
     5341 gacgaccggt gagctgcgcg accagcagac cttgcagaag gatcaggacg cgatcggttc
     5401 ggcccagacg gcgcaagcgc aaggtattgc caaggacaat caggcgatca cgaatgcgca
     5461 gcagcaggtg acgaacgccg agctgtcgga acagtcgacc gagaacaaca acgcgcagaa
     5521 gacggcggcc ccgctgtcgt cgaccgtgca gaccgatcag gcgcaggtcg agcaggcgca
     5581 ggcacaggtc gccactgcta agaccactct cgatgcgacg gcgttactcg cctcggccga
     5641 tggcaccgtc ggcgccatca acgacaccgt cggtcagacc gtcactgccg gcacgacgtc
     5701 gtcggccagc acctcgtcgt cgacgtcgaa ctcctcaagc agctcgtctg gcttcatgac
     5761 gctcaccaac ttg
//
LOCUS       DASWVF010000301         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_467029, whole genome shotgun sequence.
ACCESSION   DASWVF010000301 DASWVF010000000
VERSION     DASWVF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467029"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGD55_05320"
     CDS             <1..383
                     /locus_tag="VGD55_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869794.1"
                     /translation="AEPSPAAEGYQRAGTVSGTTFWIILQGPNDLRLRTTGRLSGICN
                     SEGPFDATAGPVPVCAQSIRKIGSVFVYVLPSATTPKTLSLSDGTTADVTGIIPLART
                     LAGKKLVVYVLPPPEQIVSLAQSP"
     gene            complement(623..1888)
                     /locus_tag="VGD55_05325"
     CDS             complement(623..1888)
                     /locus_tag="VGD55_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0869795.1"
                     /translation="MSTRRVRLRLPRRTVRLRLTLWYGCLFVLSGAVMLVITNLLVRH
                     ATGNFLFVTGTGSDGSSTAAVLAGGGSGTVTAIPSPVGPNDPTQLRAQAQQQAKQLVD
                     EALHQHTAELHQLLIQSLIALAVMSVISIVLGWIVAGRALRPLRTITAAARDISSNNL
                     HERLALAGPEDELKELGDTFDALIARLERSFRSQRQFVANASHELRTPLTLQHALLES
                     ALLDPDKTLPGWRVTGERALKASKEQERLIGALLTLARSERGLSHHEPFDLSIVTTAI
                     LSGREADANQRGVHVSARLDPAPTTGDLELAERLVANIIDNALRHNVVDGRVEVVTES
                     SNGHAVISVRNTGPSIPPSEIDRLFEPFQRADGERTSYDGGLGLGLSIVQSIATAHDA
                     SIRVSAQVTGGLDVVVQFPLAISQAALAR"
     gene            complement(1885..2538)
                     /locus_tag="VGD55_05330"
     CDS             complement(1885..2538)
                     /locus_tag="VGD55_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869796.1"
                     /translation="MRVLLVEDHVELARTIAGVLRREGMAVDLAFDGQQALDRAAVSR
                     YDVIVLDRDLPVVHGDDVCRSIMSDGSESRVLMLTAAGSLEERVGGLGLGADDYLPKP
                     FAFVELVARIRALARRGGTPVPPTLVSGDLRVDPAQRTAVRAGRPLELSRKELAVLEY
                     LLAAQGRPVTAEELLEHVWDEAADPFTTTVKVTIRRLRTKLGDPPLIETVAQGGYRI"
     gene            complement(2611..2868)
                     /locus_tag="VGD55_05335"
     CDS             complement(2611..2868)
                     /locus_tag="VGD55_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869797.1"
                     /translation="MGDALLAGVAFDPEPDVTATLDAAAVSWATLGPAELGLHPVAPS
                     TRHAAAVAINVNLDFESIATLGFLLYRSVALAGSGVVRQKW"
     gene            2888..3283
                     /locus_tag="VGD55_05340"
     CDS             2888..3283
                     /locus_tag="VGD55_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869798.1"
                     /translation="MAYSECMRSHGVPDFPDPVGNSLQVRVSPGSDLDPNSPVMQTAQ
                     KACQSLQPGGGQGGTIGTAQRDQALKYSACMRSHGVPNFPDPVFSNGGAQLKITGIDP
                     NSAQFQAAQKACQSLEPGGAGAQTNSNGP"
     gene            3280..3852
                     /locus_tag="VGD55_05345"
     CDS             3280..3852
                     /locus_tag="VGD55_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869799.1"
                     /translation="MRRGAARFGPRALIVASAACGALVIAACGSSAAHPGVAQLAASG
                     STSAAAGSPAGPTTGKNAATPLAHAECMRSHGVPDFPDPGPIHIDVGAHPDLDPSSPA
                     FVAAQKACVALEPGGTTGNGVTPAMQAAALAYSACMRTHGFPNFADPVFDSRGEHVSI
                     NGIDTDSAQFQNADKTCQTSSGLAQVSGHS"
BASE COUNT          722 a         1272 c         1251 g          696 t
ORIGIN      
        1 ccgcagagcc gtcgccagca gctgaaggtt accagcgtgc ggggacggta agcggcacaa
       61 cgttttggat catccttcaa ggacccaacg acctgcgtct acgcacgacc ggccgtttaa
      121 gtggcatctg caatagcgaa gggcccttcg atgctacggc cggacccgtg ccggtctgtg
      181 cgcagagcat acgcaagatc ggatcggtct tcgtttacgt attgccaagc gcgacaacgc
      241 cgaagacgtt gtccctaagc gacggcacga cggcagacgt gacaggcatc attccgttgg
      301 cccggacatt ggcgggtaag aagctcgtcg tgtacgtgct gccgccgcct gagcagatcg
      361 tcagcctcgc gcagagcccc tagcgtgggg gcgatgacgc ggtcccccgg tcctcgtgat
      421 gacgctcaga atgcctgcgt gtcttatcgt cacgctattc agacgctcgt ccgatccacc
      481 aaccaagcac tcgtaatgcg taggtctgga gttcgaatct ccaaggcggc tccctgcgcg
      541 ttgaatgcgc cacgcagccc aggggttgcc gcagctattc gacggtctca gcgtggtcga
      601 tggtggtcca gaagtagccg cgtcaccgtg cgagtgctgc ctgactgatg gccaacggga
      661 actggaccac gacgtcaagc ccgccggtga cctgggcgct gaccctgata ctggcgtcgt
      721 gagcggtcgc gatcgactgc acgatggaca gtccaagacc caggccgccg tcatagctag
      781 ttcgttcgcc gtccgcgcgc tggaagggct cgaacagtcg gtcgatttca gacgggggga
      841 tggacgggcc ggtgttgcga accgaaatca ccgcgtgccc gttgcttgac tcggtgacga
      901 cctccacgcg accgtcgacg acgttgtgcc gcaacgcgtt gtcgatgatg ttggcgacga
      961 gtcgctcggc gagttctaga tcgcctgtcg tgggcgccgg atcaagcctg gctgaaacgt
     1021 gcacgccgcg ttggttcgcg tcggcctcgc ggccgctgag tatcgccgtt gtgacaatcg
     1081 aaaggtcgaa cggttcgtga tgactgagcc ctcgttcgct ccttgcgaga gtgagcaacg
     1141 cgccgatcag ccgctcctgc tccttgctgg ctttgagcgc gcgctcgccg gtaactcgcc
     1201 agcccggcaa cgtcttgtcg gggtcgagga gcgcggactc aagcagcgca tgctgcagcg
     1261 tcagtggcgt acgcaattcg tgcgaggcgt tggcgacgaa ctgccgctgc gaccggaagg
     1321 accgctcgag tcgagcgatc agcgcatcga aagtgtcccc gagctccttg agctcgtctt
     1381 ctggtcccgc gagagcgagc cgttcgtgca agttgttcga ggagatgtca cgtgccgctg
     1441 cggtgatcgt tcgcagcggg cgcagcgcgc gaccggccac gatccagccg agcacgatcg
     1501 agatcaccga catgaccgcc aacgcgatca acgactggat gagcagttgg tgcagctcgg
     1561 cggtgtgctg atgaagcgcc tcgtcgacga gttgcttggc ctgttgctgg gcctgcgccc
     1621 tcaactgggt cgggtcgttt ggtccgaccg gagacggtat tgccgtcact gtgcccgacc
     1681 cgccacccgc aagaacagcc gcggtactgc tgccgtctga gccggtgcct gtcacgaaca
     1741 ggaaattgcc ggttgcgtgt cgcaccaaca ggttggtgat gacgagcatg acggcgcccg
     1801 ataaaacgaa caagcatccg taccacaacg tcagacgcag ccgtacggtg cggcgcggca
     1861 ggcgcaaacg aactcgtcga gtactcatat tcgataacca ccctgtgcga ccgtttcgat
     1921 gagaggcggg tcaccaagct tcgttcgcag tcgcctgatc gtgaccttca cggtagtcgt
     1981 gaacgggtca gcagcttcgt cccacacatg ctccaacaac tcttcagccg tcacaggccg
     2041 accttgcgca gcaagcaggt attcgaggac ggcgagctcc ttgcgactca gttcgagcgg
     2101 acgaccggca cggacggcgg tgcgctgcgc cgggtcgacg cgcaggtcgc cactgacgag
     2161 cgtcggcggg accggcgtcc cgcctcgtcg tgcaagcgca cgaatccgtg ccaccaactc
     2221 gacgaacgcg aacggcttcg gcaagtagtc gtcggcgccc agcccaagcc cgcccactcg
     2281 ctcttcgagg ctgcctgcgg cggtcagcat cagcactcgg ctctcggatc cgtcgctcat
     2341 gatgctgcgg cagacgtcat caccatgcac gaccggcagg tcgcgatcca acacgatgac
     2401 gtcgtaacgg ctcaccgctg cgcggtcgag tgcctgctgc ccgtcgaagg ccagatcgac
     2461 agccattcct tcccgccgca gcactcccgc gatggtgcgc gcgagctcga cgtggtcttc
     2521 gaccagcagt actctcacgc gcttcttccc atccgtccag gatgcatcgc cagcggtgac
     2581 aaagcggtga caagtcgtgt caccgcgccg tcaccacttc tgtcgtacaa ctccactacc
     2641 agcaagagcc accgacctgt agagcaggaa gccgagagtc gcgatggatt cgaaatccag
     2701 gttcacgttg atcgccactg cagcggcgtg cctcgtgctc ggcgctaccg ggtgcagccc
     2761 gagttctgct ggacctagtg tggcccagct cacggcagct gcgtcgagtg ttgccgtgac
     2821 atcgggctcg gggtcgaatg caacgcctgc gagtagcgcg tcgcccaaga agggagaggc
     2881 gggtctcttg gcttactcgg agtgcatgcg ctcacacggc gtgcccgatt tccccgatcc
     2941 ggtcgggaac agtttgcaag tcagggtgag ccccgggtcg gacctcgatc ccaactcacc
     3001 ggtcatgcag accgcgcaga aggcgtgcca gtctcttcaa cctggagggg gccagggagg
     3061 cacgatcggc accgcccaaa gggatcaggc gttgaaatac tcggcctgca tgcgctcaca
     3121 cggagtgccc aacttcccgg atcccgtatt cagcaacggt ggagcccaac tcaagatcac
     3181 tggtatcgac ccgaattcgg cgcagttcca agccgcgcag aaggcctgcc agtcgctgga
     3241 acctggcggc gctggtgccc aaacgaacag caacgggcca tgagacgcgg tgcagcgcgt
     3301 tttggaccac gcgccctcat tgttgcgagt gctgcgtgcg gcgccctcgt catcgccgcc
     3361 tgcggtagca gcgccgcgca ccctggcgtc gcgcagctcg ccgccagcgg ttcgacgtcg
     3421 gcagcggcag gctcgccggc aggcccgacg actgggaaga atgcggcgac gccgctcgcg
     3481 cacgcagagt gcatgcggtc acacggcgtg cctgactttc ccgatcccgg tccgattcac
     3541 atcgacgttg gagcgcaccc cgatctggat cccagctcgc cagcgttcgt ggcagcacag
     3601 aaggcatgcg tcgcactgga accgggaggc accacgggta acggggtcac ccctgccatg
     3661 caagcggctg cattggcgta ttccgcatgc atgcgaacgc acggctttcc gaacttcgcc
     3721 gaccccgtgt tcgacagccg cggcgagcat gtctcgatca acgggatcga cacggactcg
     3781 gcgcagttcc aaaatgctga caagacctgc cagacgtcgt ccgggctggc gcaggtgtcg
     3841 ggccacagtt agttccggtt agttccggtt agttcagagc cagagccggc accgtatcgg
     3901 cgggggacgc gaatcgcacg gtgatcttca gaccgccgtt a
//
LOCUS       DASWVF010000302         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_110255, whole genome shotgun sequence.
ACCESSION   DASWVF010000302 DASWVF010000000
VERSION     DASWVF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110255"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGD55_05350"
     CDS             <1..402
                     /locus_tag="VGD55_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0869800.1"
                     /translation="AEAKSALNGLFLGLGAVALLIGAVGVANIMVISVLERRSEIGLR
                     RALGATKGHIRTQFLSEATLLATLGGAVGVGLGALATAIYASTKGWAIVIPTLAWGGG
                     LGAALVIGAFAGLLPALRAARMSPTQALWSV"
     gene            509..895
                     /locus_tag="VGD55_05355"
     CDS             509..895
                     /locus_tag="VGD55_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869801.1"
                     /translation="MAATYTVEWLQPGILLMRRTGLMSVDEATEYAATAARMITTAPA
                     RWGAVVDVRGAVAQTDEVQQIVQRIIQAFVSKNVARIAMVSSSAITEMQQRRVAIAPG
                     MHDPKTLAFYRDFDEAVVDVKTTIAS"
     gene            complement(899..1279)
                     /gene="pcaC"
                     /locus_tag="VGD55_05360"
     CDS             complement(899..1279)
                     /gene="pcaC"
                     /locus_tag="VGD55_05360"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEY0869802.1"
                     /translation="MSPDDPTFEAGIKVRRAVLGDAHVDRAMANADAFTGDFQDFITR
                     YAWGSIWSRPGLGRRERSLITLAILAALGHTEEIPMHVRGALTNGVTREEISEVFLHT
                     AVYAGVPSANNAFAVAKKTLDELA"
     gene            complement(1276..2040)
                     /gene="pcaD"
                     /locus_tag="VGD55_05365"
     CDS             complement(1276..2040)
                     /gene="pcaD"
                     /locus_tag="VGD55_05365"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY0869803.1"
                     /translation="MSPLGYDVQGAADAPVLVLAPSLGTHPDMWSPQVGLFAEQFRVV
                     RVELRGHCGDPPLGPYTIPSIASDILATLDGLGVSQFSFCGLSLGGMAGMWLAAQVPS
                     RVERLALCCTSAHMPPAQGWLDRAATVRRSGMAVIADAVVGRWFTPDFAAQSPGVVTW
                     AREMLLATPTEGYAGCCEAIAAMDLRPILEAITAPTLVLAGALDPSAPPSHAEVIVGA
                     VAEAQLIVVPSVSHLATAQEPDVCAQILLDHLGGTS"
     gene            complement(2037..3275)
                     /locus_tag="VGD55_05370"
     CDS             complement(2037..3275)
                     /locus_tag="VGD55_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY0869804.1"
                     /translation="MATPSRFDFKGSTRLSSSISEPTSGGLFDGIYGDAAVDAATSDH
                     AWVQAMLDVEAALAFAAARVGLIPLAAASAISEVCHVELVDMAEIARGAVADATPVPA
                     LVRSLRDLVPERARSSVHFGATSQDVLDTAMCVMSKRAVAHIAASASNVVRALASLAL
                     RHRSTHQVGRTLLQHASVTTFGAACAGRLVALDDALLGLSSVCDNQLAVQLGGAVGTL
                     SAAGDSALELLTEFALLLELPEPVVPWHTSRGRFASLASALGVLAGELAAVAQDLVLL
                     SSNEIGEVSLAAPGGSSAMAHKRNPAAAVLALACAHRVPGLVATVFAGMPQELQRSAG
                     RWQAEWGTLRNELRLVGGTALHTFNAVQGLHVHEDAMAKHVDELLAATGGAGDDIGSA
                     TVFVDRALEAHSRRTDATGS"
     gene            complement(3214..>3629)
                     /gene="pcaG"
                     /locus_tag="VGD55_05375"
     CDS             complement(3214..>3629)
                     /gene="pcaG"
                     /locus_tag="VGD55_05375"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY0869805.1"
                     /translation="AGLIESWQADPAGHFHHPDDAIGFAADPVPGFRGFGRGATGPEG
                     EWEICILKPGRVAASGGVLQAPHLDVTVLARGLLDRVVTRIYFGDEAEANAADPVLRS
                     VPEHRRGTLIADVTADGYTFEIRLQGEHETVFFDL"
BASE COUNT          646 a         1222 c         1210 g          551 t
ORIGIN      
        1 gcagaagcaa agagcgcttt gaacggtctt ttcctcggcc tcggtgcggt tgcgctgttg
       61 atcggtgcgg tcggcgtcgc gaacatcatg gtgatctcgg tcctcgaacg ccgctctgag
      121 atcgggttac gtcgcgcact cggagcgacg aagggtcaca tccgcacgca gttcctttcg
      181 gaagccacgc tgctcgcaac acttggcggc gcggtcggcg taggcctcgg cgcactagct
      241 accgccatct acgcgagcac caaaggatgg gcaatcgtca tcccaactct cgcgtggggt
      301 ggtggccttg gagcggcgtt ggtcatcggc gccttcgcgg gtctgctgcc ggctttgcgg
      361 gcggctcgga tgtcacccac ccaggccctc tggagcgtct gagtgccggg acgcgaacct
      421 acgaacacgg cgctcaggat cttcgcccgg acgccgatat acccaagggc tacccgacgg
      481 gggcggccag ggacgagcga ggtgaaccgt ggcagctacc tacaccgtgg agtggcttca
      541 gcctggcatc ctgctgatgc gacgcaccgg cctcatgtcg gtcgacgagg cgactgagta
      601 cgcggcgacc gcagctcgca tgatcacgac tgcccctgcg cgttggggtg cggtggtcga
      661 cgtccgtggc gccgtcgcgc agactgacga ggtgcagcag atcgtccagc gcatcatcca
      721 ggcgttcgtg tcgaagaatg tcgcgcgtat agcgatggtc tcgagcagcg cgatcaccga
      781 gatgcagcag cgccgggtcg cgatcgcgcc gggcatgcac gaccccaaga cgctcgcgtt
      841 ctaccgcgac ttcgatgagg ccgttgtcga cgtcaagacc accatcgcca gctagacgtt
      901 acgcgagctc gtcgagggtt ttcttcgcca cggcgaatgc gttgttcgct gaaggcactc
      961 ccgcatagac cgcggtgtgt agaaacacct cgctgatctc ctcgcgggtg accccgttcg
     1021 tcaacgcgcc gcgcacgtgc atcggtatct cctccgtgtg ccctagcgcc gcaagaatcg
     1081 cgagcgtgat caggctgcgc tctcggcggc cgagacccgg gcggctccag atcgagcccc
     1141 acgcgtagcg cgtgatgaag tcttgaaagt caccggtgaa tgcatcggca ttcgccatcg
     1201 cgcggtcgac gtgcgcgtcg ccgagcaccg cgcgccgcac cttgatgccg gcctcgaaag
     1261 tcgggtcgtc tgggctcatg acgtgcctcc gaggtggtcg aggagtatct gcgcgcaaac
     1321 gtctggctct tgtgccgtcg caagatgaga aacagagggg acgacgatca gctgcgcttc
     1381 tgcaacagca ccgacgatga cctcggcgtg agacggggga gccgacgggt cgagcgcgcc
     1441 cgccagcacc agagtcggtg cagtgatcgc ctcgagaatc ggccgcaggt ccatggccgc
     1501 gatcgcctcg cagcacccgg cgtagccctc cgtcggcgtt gcgagcagca tctcgcgcgc
     1561 ccaggtcacg accccgggtg actgcgccgc gaagtcgggc gtgaaccagc ggccgaccac
     1621 cgcgtcggcg atgaccgcca tgcccgaccg gcgaaccgtc gcggcgcggt cgagccatcc
     1681 ttgtgccggc ggcatgtggg ccgacgtgca gcacaaggca agcctctcga cgcgcgatgg
     1741 aacctgtgct gcgagccaca tgcctgccat gccgccgagc gacagcccgc agaaggaaaa
     1801 ctgcgagacg cccaacccgt cgagcgtcgc caatatgtcg cttgcgatcg aggggatcgt
     1861 gtaaggaccc aagggcggat cgccacagtg gccgcgcaac tcgacgcgca ccacccggaa
     1921 ttgctctgcg aagagcccaa cctgtggcga ccacatgtcg gggtgcgtgc cgagcgatgg
     1981 ggcgagaaca aggacgggcg cgtcggcagc gccctggacg tcgtacccga gagggctcat
     2041 gagccggtag cgtccgtgcg ccgcgagtgc gcttcgagtg cgcggtcgac gaacacggtt
     2101 gcggatccga tgtcgtcgcc agctccgccg gtcgcggcca gcaactcatc gacgtgcttc
     2161 gccatcgcgt cctcgtgtac gtggaggcct tgcacggcgt tgaaggtatg caacgccgtg
     2221 ccgccaacga gtcgcaactc gttgcgcagc gttccccact cagcctgcca gcgaccggcc
     2281 gagcgctgga gctcttgcgg catgcccgcg aacacggtcg ccaccagccc cggcacccgg
     2341 tgcgcgcacg ccagcgcaag aaccgctgct gccgggttgc gcttgtgtgc catcgctgac
     2401 gagccgccgg gcgcggccag cgacacctcg ccgatctcgt tggacgacag cagcacgagg
     2461 tcttgtgcga cggccgcaag ctcgccggcg agcacaccga gcgcagacgc gagtgaggca
     2521 aaacgtccgc gcgacgtgtg ccacggcacc acgggctcgg gcaactccag cagcagcgca
     2581 aattcagtca agagttccag cgcgctatcg ccggcagcag acaacgtgcc caccgccccg
     2641 ccgagctgta cggccagctg gttatcgcaa accgacgaga ggccgagcaa cgcgtcgtcc
     2701 aacgcaacta ggcgaccagc acaggccgcg ccgaaggtcg tcaccgacgc gtgctgaagc
     2761 agcgttcgtc ctacctgatg ggtcgagcgg tggcgcaagg caagactcgc gagcgcacgc
     2821 acgacgttcg acgcacttgc ggcgatgtgc gcgaccgctc gcttcgacat gacgcacatt
     2881 gcggtgtcga gaacgtcctg actggtcgcg ccgaaatgca ccgacgaccg ggcacgctct
     2941 ggcacgaggt cgcgcaggct gcgaaccagc gcaggcaccg gtgtcgcatc ggcgaccgct
     3001 ccgcgcgcga tctcggccat gtcgacgagc tcgacgtggc acacctccga gatcgccgat
     3061 gcggccgcga ggggaatcag gccgacgcgg gctgccgcga acgcgagcgc cgcctcgaca
     3121 tcgagcatgg cctgcaccca cgcgtgatcg gaggtggccg cgtcgacggc agcgtctccg
     3181 tagatgccgt cgaacagacc acctgatgtg ggctcagaga tcgaagaaga cagtctcgtg
     3241 ctccccttga agtcgaatct cgaaggtgta gccatctgcc gtgacgtcag cgatgagcgt
     3301 cccacgccgg tgctccggca ccgagcgcaa caccgggtct gctgcgttgg cttctgcctc
     3361 gtcgccgaaa tagatgcgcg tcaccacgcg atcgaggagg ccgcgggcga gcacggtgac
     3421 gtcaagatga ggcgcttgca aaacgccgcc ggaagccgcg acccgcccgg gcttcaagat
     3481 gcagatctcc cactcgcctt caggccccgt tgcgccacgc ccgaaacccc ggaatcccgg
     3541 aaccgggtcg gccgcgaaac cgatcgcgtc gtcggggtgg tggaaatgac cggcggggtc
     3601 ggcctgccaa gactcgatca gaccagcgg
//
LOCUS       DASWVF010000303         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_364877, whole genome shotgun sequence.
ACCESSION   DASWVF010000303 DASWVF010000000
VERSION     DASWVF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364877"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGD55_05380"
     CDS             <1..361
                     /locus_tag="VGD55_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY0869806.1"
                     /translation="KVRAAASLVAQLRPELPLVGPIQYDAAIDPSVAASKLPGNDVAG
                     HANVIVFPDLNTGNATYKAVQRSTNAIAIGPVLQGLPRAVNDLSRGCTVDDIVNTVAI
                     TAIQAQHLPGHENLPVT"
     gene            complement(379..1053)
                     /locus_tag="VGD55_05385"
     CDS             complement(379..1053)
                     /locus_tag="VGD55_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869807.1"
                     /translation="MPSTEAQITVFLLDDHEIVRRGVKELLEAEPDIVVIGEGSTAAE
                     ALARVPALRPQVAVLDVRLPDGDGVSVCRDLRSKLPDLACLMLTSFADDDALYDAILA
                     GAAGYVLKQIKGSDLVNAVRTIAGGGSLLDAKTTARIMQRLRDDAADREKADPFAALT
                     NQEHRILDLIGEGLTNRQIGERMFLAEKTVKNYVSSLLAKLGLERRTQAAILATESKQ
                     DKHPAP"
     gene            1160..1585
                     /locus_tag="VGD55_05390"
     CDS             1160..1585
                     /locus_tag="VGD55_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0869808.1"
                     /translation="MSVDASGLEMLSESEALALLATVPLGRIVYSDRALPFVAPVSFI
                     LDGSDIVIRTGRRSNLATHGPGNIVAFQVEDIAMPTGSGWTVVVTGRLQLVHDAAEIS
                     RLSALNLTSWAPAESDRYLQLRPELITGRRITPDVQPAD"
BASE COUNT          288 a          530 c          534 g          314 t
ORIGIN      
        1 gaaggtgcga gcagctgcga gtttggtggc gcagctacgt ccggaactcc cgctggttgg
       61 tccgatccaa tacgacgcgg cgatcgatcc gtcagtagcc gcgtcgaagt tgccaggaaa
      121 cgacgtggcc ggtcacgcga acgtcattgt ctttcccgat ctgaatacgg gcaacgccac
      181 ctacaaggcg gtgcagcgat cgacaaacgc gatcgccatc gggcccgtct tgcaagggct
      241 accccgagcg gtcaacgacc tttcgcgtgg ttgcacggtt gacgacatcg tcaacacggt
      301 cgcgatcacg gcgatccagg cacagcatct accgggacat gagaatctgc ccgtgacgta
      361 aaggcctgac gtaaagggct acggagcggg gtgcttgtcc tgcttgcttt cggtggccag
      421 aatcgcggcc tgggtgcgac gttcgaggcc gagtttggcg agcagcgacg acacgtagtt
      481 cttcaccgtc ttctcggcaa ggaacatccg ttcgccgatc tgccgattgg tcaggccttc
      541 accgatcagg tcgagaatcc ggtgttcctg gttggtcagc gcggcaaacg gatcggcctt
      601 ctcgcgatcg gccgcgtcgt cgcgcagtcg ctgcatgatg cgcgctgtgg tcttggcgtc
      661 gagcagggat ccgccgcccg cgatagtccg taccgcgttg accaagtcac tgcccttgat
      721 ctgcttcagc acgtatcccg cggcgccagc caggatcgcg tcgtagagag cgtcgtcgtc
      781 cgcgaatgac gtgagcatca gacaggcgag gtcgggcaac ttcgaccgca ggtctcggca
      841 cacactgacg ccgtcaccgt cagggaggcg gacatcgagg acggcgacct gcgggcgtag
      901 agcgggcact cgggcaagtg cttcggcggc ggtagagccc tcaccgatca cgacgatgtc
      961 gggctcagcc tctagcagtt ccttgacccc acgccggacg atctcgtgat cgtcgagcag
     1021 gaacacggtg atctgggctt cagtgcttgg cacgctacct cccgatacgg ctgcggcctc
     1081 aaccgtagct cccatggttc ccgacccgaa gggaccttgg actctgcggc gcttcgaggc
     1141 aaccgccgaa catggatcta tgtctgtcga cgcgtcgggc ctcgagatgc tctccgaatc
     1201 ggaggcgctc gccctgctgg caaccgtgcc gctcggccgg atcgtgtatt cggaccgcgc
     1261 gttgccgttt gtcgccccgg tgagtttcat tctcgacggc tcagacatcg tgattcggac
     1321 aggtcgccgg tcgaacctgg ccactcacgg gcctggcaat attgtggctt tccaggttga
     1381 ggatatcgcc atgccgaccg gctccgggtg gacagttgtg gtcaccgggc gccttcaact
     1441 cgtccacgat gcggccgaga tcagccggct cagcgcactg aacctcacca gctgggcacc
     1501 ggcggaatcc gaccgctacc tgcagctgcg tcccgaactc atcaccggcc ggcggatcac
     1561 ccctgatgtg cagccggctg actagtcgtc gccgaggggc actcgccaac tgagttcggt
     1621 gccgccctca ggagcctgct gggcggtgaa cgtgccgtcg agggtg
//
LOCUS       DASWVF010000304         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_339551, whole genome shotgun sequence.
ACCESSION   DASWVF010000304 DASWVF010000000
VERSION     DASWVF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339551"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VGD55_05395"
     CDS             <1..1765
                     /locus_tag="VGD55_05395"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0869809.1"
                     /translation="EGGGPHLDLLRRTSHWLMKQPDLDEEALRLQVHGKDLVVQRQTM
                     ADTVAPVTVTSPSGATRELTLTAGDAGIWSSTFPASELGLWQATDGTLKALINVGPTN
                     PKEFSEVTSTTDMLKPLAQATGGDAQRVADVSGVELPRIVPVRSSGIFHGDGWMGVRM
                     RDASVVKGVGVLPVFAGLIGLLLLLGACGKSAQVPLQSWLGDAMEGPTPVSALIHAAT
                     MVTAGVYLIARSNFIFDVSPNARLAVAIVGTVTLVFGAIIGCAKDDIKKALAASTMSQ
                     IGYMMLAVGLGPAGYAFGIAHLLTHGFFKAGLFLGAGSVMHGMNDEVDMRRYGGLRKF
                     MPFTFITFGLGYLAIIGFPGLAGWFTKDAIIEAAFHKGGTSGAIFGGCALAAAGLTAF
                     YMTRVMLMTFFGEPRWADDAHPHESPNVMTVPMWILAIGSVGGGYVLILSGRLAKFLA
                     VVVPRPADIAFKPIGVIGLATAGVVLLGVVGSWLMYGRRPVPAVAPVGSPLTIAARRD
                     LLGDALNESVFMRPGQYLTRLLVYFDNRGIDGFVNGFAAFVGGTSSRVRRFQSGFVRS
                     YALSMFGGIVLVLAALLLVKG"
     gene            1771..>2565
                     /locus_tag="VGD55_05400"
     CDS             1771..>2565
                     /locus_tag="VGD55_05400"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0869810.1"
                     /translation="MSFPWLTVIGVVPLVGAGVVSALPSANTKLLKQVALGVSLVTLA
                     LTIAMATQFAPRGDRFQFNQDVSWIPAFGIHYAVGIDGIALVLILMTAVLWPVVILAS
                     WNECETTKRSVRTYLSLLVAMETLLIGVFAATDVFLFYVFFEAMLIPMYFIIGSAGTP
                     RSSYAALKFLLYSLFGGLLMLAAVIGLYVTGGHTFLYSSLQHLHLAGNDEKWLFLGFF
                     VAFAVKAPLWPFHTWLPDAAAESPVGGTVLLVGVLDKVGTFGFLRYC"
BASE COUNT          367 a          814 c          832 g          552 t
ORIGIN      
        1 cgaaggcggc ggcccgcacc tcgatctgtt gcggcggacc tcgcactggt tgatgaagca
       61 gcctgatctc gacgaggaag cgttgcggct gcaggtccac ggcaaggatc tggtggtgca
      121 gcgccagacc atggccgaca ccgtagcccc ggtgacggtg acatcgcctt cgggcgcgac
      181 acgcgaactg actctcacgg ccggcgacgc cggaatttgg agttcgacct tcccagccag
      241 cgaactcggc ttgtggcaag ccaccgacgg tacgctgaaa gcgctgataa atgtcgggcc
      301 gaccaacccg aaggaatttt ccgaggtcac ctcgaccacc gacatgctca agccgctggc
      361 gcaggcgacc ggcggcgacg cacagcgcgt ggctgatgtc tcgggcgtcg aactgccgcg
      421 catcgttccc gtgcgttcgt ccggcatttt ccacggcgat ggctggatgg gcgtgcggat
      481 gcgcgacgcc agcgtggtca agggcgtcgg ggtactgcct gtcttcgccg gactgatcgg
      541 cctgttgctg ctgctcggcg cgtgtggcaa gtccgcccag gtaccgctgc agtcgtggct
      601 cggcgacgcg atggaaggcc cgactccggt atcggcactc attcatgcgg caacgatggt
      661 gaccgcaggt gtctacctga tcgcgcggtc gaacttcatc ttcgacgtct cgccgaatgc
      721 ccggctcgcg gtggcgatcg tcggcaccgt gacgcttgtg ttcggtgcga tcatcggctg
      781 cgcgaaggac gacatcaaga aggctctcgc ggcgtccacg atgagccaga tcggctacat
      841 gatgttggcc gtcgggctcg ggccggccgg gtacgcgttc ggcatcgcgc acctgctgac
      901 gcacgggttc ttcaaggccg ggctgttcct cggcgccggt tcggtgatgc atggcatgaa
      961 cgacgaggtc gacatgcgcc ggtacggcgg ccttcgcaag ttcatgccat tcacgttcat
     1021 cacgttcggg ctcggctatc tcgcgatcat cggatttccc ggcctggccg gctggtttac
     1081 caaggacgcg atcatcgagg ccgcgtttca caagggcggc acctcgggcg cgatcttcgg
     1141 tggctgcgcg ctcgcagcgg ctggcctgac tgccttctac atgactcgcg tcatgctgat
     1201 gacgttcttc ggagagccgc ggtgggccga cgacgcgcac ccgcacgagt cgcccaacgt
     1261 catgacggtc ccgatgtgga tcctggccat cggttcggtc ggcggcggtt acgtgttgat
     1321 cctcagcggt cggctcgcga agttcctcgc ggtggtcgta ccgaggccgg ccgacatcgc
     1381 cttcaagccc atcggcgtga tcggtctcgc gacggcgggc gtggtcctgc tcggtgtcgt
     1441 cggttcgtgg ctcatgtacg ggcgacgccc tgtcccggcg gtcgctcccg tcggaagccc
     1501 gctgacgatc gctgcccgcc gcgaccttct cggcgatgcc ctcaacgagt cggtcttcat
     1561 gcgcccaggg caatacctca cgcggctgct cgtgtacttc gacaaccgcg gcatcgacgg
     1621 ctttgtgaac ggcttcgccg cctttgtagg cgggacgtcg agtcgtgtcc gtcgcttcca
     1681 aagcggcttc gtccggtcgt acgcgttgtc gatgttcggc gggatcgtgc tcgtgttggc
     1741 tgcccttctg ctggtgaagg gctgagtgcg atgagtttcc cctggctcac ggtgatcggt
     1801 gtcgttccgc tcgttggcgc tggagtcgtc tcggcgttgc catcggcgaa caccaagttg
     1861 ctgaagcaag tcgccctcgg agtctcgctg gtgacgttgg ctctgacgat tgcgatggcg
     1921 acgcagttcg ctccgcgtgg tgacaggttc cagttcaacc aggacgtcag ctggattccg
     1981 gccttcggta tccattacgc cgtcggcatt gacggcattg cgctcgtgct cattttgatg
     2041 accgctgtct tgtggcccgt ggtgatcctt gcgtcgtgga acgaatgcga gaccaccaag
     2101 cgaagcgtca ggacctacct gtcgttgctc gtggcgatgg agacgttgtt gatcggcgtc
     2161 ttcgccgcga ccgacgtctt cctgttctac gtgttcttcg aagcgatgct gatcccgatg
     2221 tacttcatca ttggcagcgc cggaacgcct cgctcgtcgt atgcggcctt gaagttcttg
     2281 ctgtacagcc ttttcggcgg tctcctcatg ctcgccgcgg tgatcgggct atacgtaacc
     2341 ggcggccaca ccttcctcta ctcctcgctc cagcatctgc atctcgcggg caacgacgag
     2401 aagtggctgt tcctcggatt cttcgtcgca ttcgctgtca aggcgccgct gtggcccttc
     2461 cacacctggc tgcccgacgc cgcggccgag tcacctgtcg gcggcacggt gcttctcgtc
     2521 ggtgttctcg acaaggtcgg cacgttcggg ttccttcgct actgc
//
LOCUS       DASWVF010000305         5305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_492322, whole genome shotgun sequence.
ACCESSION   DASWVF010000305 DASWVF010000000
VERSION     DASWVF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492322"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VGD55_05405"
     CDS             <1..1122
                     /locus_tag="VGD55_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY0869811.1"
                     /translation="PRAVKQLVKLGIDTSSEAGWLQNKGLRILAGGMRVELPWPELSS
                     FPDYGLVRTRLDFDQILARRAQKLGVALHEQTPATQPVIDGGSGRVIGVMARPAGAER
                     DVMFKAQVIVAADGNSSRFSLALGLHRRENRPMGVAYRRYFESPRHDDDWLESWLELW
                     DRFEGKDRLLPGYGWVFGMGDGTSNVGLGILNTSSAFRNVDYRQLLKRWTAGMPKEWG
                     FDEEHARGAVRGAALPMGFNRKPHYDRGVLLVGDAGGMVNPSNGEGIAYAMESGELAA
                     EIIAQALARPAGTPRERALHGYPKALDDRYGGYFTLGRGFVNVIGKPGVMRLLTRHGM
                     SHPTIMRFTLKMLANLTDPKGGDAMDRIINGLSRIAPAA"
     gene            1200..1568
                     /locus_tag="VGD55_05410"
     CDS             1200..1568
                     /locus_tag="VGD55_05410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0869812.1"
                     /translation="MTSYFSYVPLLALFLLAAGFAVFSILMGSLSGPKRYNRAKYDAY
                     ECGIEPTPQPVGGGKFPVKYYLTAMLFIVFDIEIIFLYPWAVAFQHLGVFGLVEMVLF
                     ILTVFVAYVYVWRRGGLEWD"
     gene            1578..2132
                     /locus_tag="VGD55_05415"
     CDS             1578..2132
                     /locus_tag="VGD55_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0869813.1"
                     /translation="MGLEENLPSGFLLTSVEKLAGTARKNSLWPASFGLACCAIEMMA
                     SGAGRYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQIYDQMVEPKWVLAMGV
                     CASSGGMFNNYAIVQGVDHVVPVDMYLPGCPPRPEMLMHAILELHERIQVMKLGPNAK
                     KQAQELEIEALEATPTHQMKGLLR"
     gene            2099..2704
                     /locus_tag="VGD55_05420"
                     /pseudo
     CDS             2099..2704
                     /locus_tag="VGD55_05420"
                     /inference="COORDINATES: protein motif:HMM:NF005856.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            2701..4032
                     /locus_tag="VGD55_05425"
     CDS             2701..4032
                     /locus_tag="VGD55_05425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0869814.1"
                     /translation="MSATTDPYAGVRETTEGRVYTVTGGDWDDVAADATNERAERVVV
                     NMGPQHPSTHGVLRLILELDGETVTQARLAIGYLHTGIEKNCEYRNFTQAVTFLTRAD
                     YLSPLFTESAYSMAVEKLLGITDEVPVRAQVIRVMMMELNRISSHLVWLGTGGLEVGA
                     LSAMLYAFRERELILDIFEMISGLRMNHAYVRPGGVIQDLPPGAVEKIREFVQLMPKR
                     IDEYHNLLTGQPIWIARLQGVGYLDVNACVALGVTGPLLRAAGLPWDLRKSQPYSGYE
                     TYEFDVPTQTEGDNFARFLVRMDEMRESVKIVEQCLDRLRPGPVMVEDKKIAWPAQLA
                     LGADGMGNSLDHIRHIMATSMESLIHHFKLVTEGFHVPPGQVYQPIESPRGQLGVHLV
                     SDGGTKPYRVHLREPSFVNLQAVPAVCEGSLIADVIASVASLDPVMGGCDR"
     gene            4029..4781
                     /gene="nuoE"
                     /locus_tag="VGD55_05430"
     CDS             4029..4781
                     /gene="nuoE"
                     /locus_tag="VGD55_05430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0869815.1"
                     /translation="MSNDEAKNGLPTVTLDPRLGPGAALADPLSDDAKARVRELGAQV
                     IARYPRARSAMMPLLYLVQAEQGYVSPDGIKLVAELLGLTTAEVAAVSTFYTMYKRHS
                     CGDYHVGVCTNTLCAILGGDAIFAALKGHLDVANGGTTPDGKVTLEHIECNAACDYAP
                     VVMVNWEFFDNQTVESAKELVDRLRAGDLPLPTRGAPLITHVETSRVLAGFADDLVDE
                     GPSAGPASVVGLELAAERDETAPAFDGKGTHA"
     gene            4778..>5305
                     /locus_tag="VGD55_05435"
     CDS             4778..>5305
                     /locus_tag="VGD55_05435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEY0869816.1"
                     /translation="MTFTLTPVLTANWGTPKSWTLDSYRRTGGYGSLRDALAMQPDAL
                     ISLLKDSGLRGRGGAGFPTGMKWGFIPQGDGKPHYLVVNADESEPGTCKDIPLMMASP
                     HTLLEGVLISAYAIRANQAFIYVRGEVLHVVRRLLNAVEEAKAAGFIGTNVLGSGFDI
                     DVVVHIGAGAYICGEE"
BASE COUNT          939 a         1729 c         1716 g          921 t
ORIGIN      
        1 ccacgcgcgg tcaagcaact ggtcaagctc gggatcgaca ccagctccga ggcgggttgg
       61 ctgcagaaca agggcctgcg catcttggcc ggcgggatgc gcgtcgagct gccgtggccc
      121 gagctgtcga gtttccccga ctacgggctg gtccgaacca ggctcgactt cgaccagatt
      181 ctcgcgcggc gggcccaaaa gctcggcgtc gcgctgcacg agcaaacccc cgcgacccag
      241 ccggtgatcg acggcggatc agggcgggtc atcggggtca tggcgcgccc ggccggtgcc
      301 gagcgcgacg tgatgttcaa ggcgcaggtc atcgtcgcag ccgacggcaa ctccagccgc
      361 ttttcgctag cgctcggcct gcatcgccgc gagaaccggc cgatgggcgt ggcgtatcgc
      421 cgctacttcg agtcgccccg ccacgacgac gactggctcg aatcgtggct cgagctgtgg
      481 gaccgcttcg agggcaaaga ccggctgctg cccggttatg gctgggtctt cggcatgggc
      541 gacggcacca gcaacgtcgg cctgggcatc ttgaacacgt cctccgcctt ccgcaacgtt
      601 gactaccggc agctgctcaa gcggtggacg gcggggatgc ccaaggaatg gggattcgac
      661 gaggagcatg cgcggggcgc ggtacgcggc gcggcgttgc ccatgggctt caaccgcaag
      721 ccgcactacg accgcggagt gctgctcgtt ggcgacgccg gcggcatggt gaacccctcc
      781 aatggcgagg gcatcgccta cgcgatggag tccggcgagt tggcggccga gatcattgcg
      841 caggcgttgg cgcgcccggc cggaactcct cgcgaacgag ccctgcacgg gtacccgaag
      901 gcactagacg accggtacgg cggctacttc accctgggtc gcggcttcgt gaacgtgatc
      961 ggcaagcccg gggtcatgcg gctcctgaca cgccacggca tgtcgcaccc gaccatcatg
     1021 cggttcacct tgaagatgct cgcgaacctc actgatccca agggcggcga cgccatggac
     1081 cgcatcatca acggactcag caggatcgcc ccggctgcct gacccgcgcg cgggacgcgc
     1141 ttcgcaccca cctagactga cacggcccca gtcggcacgc gagaagtgag gaaaacacgg
     1201 tgaccagcta cttcagctac gtaccgctcc tcgcattgtt cttgctcgcc gccgggttcg
     1261 ccgtcttctc catcctcatg ggcagcctca gtgggccgaa gcgttacaac cgggcgaagt
     1321 acgacgccta cgaatgcggc atcgagccga ccccgcaacc cgtcggtggc ggcaagttcc
     1381 cggtgaagta ctacctcact gcgatgctgt tcatcgtctt cgacatcgag atcatcttct
     1441 tgtatccgtg ggcggtcgca ttccagcacc tcggtgtgtt cgggctcgtc gagatggtgc
     1501 tgttcatcct cacggtcttc gttgcatacg tgtacgtctg gcgtcgtggc ggtctcgagt
     1561 gggactgagc acctgacatg ggcctcgaag agaatctgcc aagcggcttc ctgctgacca
     1621 gcgtcgagaa gcttgccggt accgcgcgca agaactcgct atggccggcg tctttcggcc
     1681 tcgcgtgttg cgcgatcgag atgatggcgt ccggcgctgg ccgatacgac ctcgcccgct
     1741 tcgggatgga ggtgttccgc gcatcgccgc ggcaggccga cctgatgatc gttgcgggcc
     1801 gggtgagcca gaagatggcg ccggtgctgc ggcagatcta tgaccagatg gtcgagccga
     1861 agtgggtgct ggcgatgggc gtgtgcgcat cgtcgggcgg catgttcaac aactacgcga
     1921 tcgtgcaggg cgtcgaccac gtcgtacccg tcgacatgta cctgccgggc tgcccgccac
     1981 gccctgagat gttgatgcac gcgatcctcg aactgcacga gcgcatccag gtcatgaagc
     2041 tcggcccgaa cgccaagaag caggcgcagg agctcgagat cgaggcgttg gaggccacgc
     2101 cgacccatca gatgaagggc ctgcttcggt gagcgacgag tccgagatgc tcccttcgac
     2161 cgcgcgcccg tacggcggat atttcgacga cgtcgccgac gcgctcgcgc tggcgtatcc
     2221 gagcgttgcg caggcagtcg agcggtcgat cgtcgatcgt ggcgagctca cgttgcacgt
     2281 gcgtcgcgag aagatgaccg atcttgcgca gcagctacgc gacgaccaag acctgcgctt
     2341 cgagttgctt tcgtcgttgt cgggtgtgca ctacccgatg gacgccgggc gcgaactgca
     2401 cgtcgtttat gaactgacct cgatgacgca caaccgccgc atcaggcttg aggtcgcagc
     2461 gccagacgac gatccgcacg tgccgtcagt tgttgcgatc tggccgaccg ctgacttcca
     2521 ggagcgcgag acctgggaca tgttcggcat catcttcgat gggcatcccg cactgacgcg
     2581 catcatgatg cccgacgact ggccggggca cccgcagcgc aaggactacc cgctcggtgg
     2641 cattccggtt gagtacaagg gcgccacgat tccgcctccc gacgagcgga ggcaatacgc
     2701 gtgagcgcca ccaccgatcc ctatgcgggc gttcgcgaga cgaccgaggg ccgggtctac
     2761 accgtcaccg gcggcgactg ggacgacgtc gcagctgatg ccacgaacga gcgggccgaa
     2821 cgagttgtcg tcaacatggg tccccagcac ccctcgaccc acggcgtgct gcgcctgatc
     2881 ctcgagctcg acggcgagac cgtcacgcag gcccggttgg cgatcggcta cctacacacg
     2941 ggcatcgaga agaactgcga gtaccgaaac ttcacgcagg cggtcacgtt cctgacaagg
     3001 gccgactatt tgtcgccgct gttcaccgag tcggcctact cgatggccgt cgagaagctg
     3061 ctcggaatca ccgacgaagt gccggtgcgt gcgcaggtca tccgcgtgat gatgatggaa
     3121 ctgaaccgta tctcctcgca cctggtctgg ctcggcaccg gcggcctcga ggtcggcgcg
     3181 ttgtcagcga tgctttacgc gttccgcgag cgcgaactca tcctcgacat tttcgagatg
     3241 atcagtggcc tgcgcatgaa ccacgcgtac gtgcggccag gcggtgtgat ccaagacctg
     3301 ccgcccggtg cggtcgagaa gatccgcgag ttcgtgcagc tgatgccgaa gcggatcgac
     3361 gagtaccaca acctgctaac cggtcagccc atctggatcg cgcgcttgca gggcgtcggc
     3421 tacctcgacg tcaacgcgtg cgtcgcgctc ggcgtcacgg gcccgttgct gcgtgcagcc
     3481 gggctgccat gggaccttcg caagagccag ccgtacagcg gctacgagac ctacgagttc
     3541 gacgtcccga cccaaacaga aggcgacaac ttcgcccgct tcctcgtgcg catggacgag
     3601 atgcgcgagt cggtcaagat cgtcgagcag tgcctcgacc ggttgcggcc gggcccggtg
     3661 atggtcgaag acaagaagat cgcctggccc gcgcaacttg cgcttggtgc cgacggaatg
     3721 ggcaactcac tcgatcacat tcgccacatc atggcgacct cgatggagtc gcttatccac
     3781 cacttcaagc tcgtcaccga gggcttccat gttccacccg ggcaggtgta tcagccgatt
     3841 gaatcgccac gggggcaact cggcgtccac cttgtaagtg acggtggtac gaagccgtat
     3901 cgagtgcacc tacgcgagcc gtcgtttgtg aacctccagg cagtcccggc ggtatgcgag
     3961 ggcagcctga tcgccgacgt catcgcatct gtcgcgagcc tcgacccggt gatgggcggg
     4021 tgcgaccgtt gagcaacgac gaggctaaga acggcttacc gaccgtcacc ctcgacccac
     4081 gcctggggcc gggcgcggcg ctggccgacc cgttgtcaga cgatgcgaag gcgcgcgtgc
     4141 gcgaactcgg cgcgcaggtc atcgcgcgtt acccacgagc ccgatcggcg atgatgccgc
     4201 tgctgtactt ggtgcaggca gagcagggtt acgtctcacc cgacggcatc aagctcgttg
     4261 ccgaactgct cggcctgacc accgccgaag tggctgccgt gagcacgttc tacacgatgt
     4321 acaagcggca cagttgcggt gattaccacg tcggtgtgtg cacgaatacg ctgtgcgcga
     4381 tcctgggcgg cgacgcgatt ttcgcagcgc ttaaaggaca tctcgacgtc gccaacggcg
     4441 gcaccacacc cgacggcaag gtcacccttg agcacatcga gtgcaacgcc gcatgcgact
     4501 acgcgccggt cgtcatggtc aactgggagt tcttcgacaa ccagaccgtc gagagcgcaa
     4561 aagaactcgt cgaccgcttg cgcgcgggcg atctgccgct gccgacccgt ggcgcgccac
     4621 tcatcaccca tgtcgagacg tcacgagtgc tcgccggatt tgctgatgat ctcgtcgacg
     4681 aaggcccctc cgccggtccg gcatcggtcg tcggtcttga gttggctgcc gagcgtgacg
     4741 agaccgcacc cgcgttcgac ggcaagggga cgcacgcatg accttcaccc tcacgccggt
     4801 gctgacggcc aactggggca ccccgaagtc gtggaccctc gactcctacc gcagaaccgg
     4861 cgggtacgga tcgctacgcg atgcgctggc gatgcagccc gatgcgctta tttcgctgct
     4921 gaaggattcc ggccttcgcg gtcgcggtgg tgccggattc cccactggca tgaagtgggg
     4981 cttcattccc caaggtgacg gcaagccgca ctacctcgtt gtcaacgcgg acgagagcga
     5041 gcccggtacc tgcaaagaca tcccgctgat gatggcgagc ccgcacacgt tgctcgaggg
     5101 cgtgctgatc tctgcctacg cgatccgcgc gaatcaggcg ttcatctacg tgcgcggtga
     5161 ggtgctgcac gtcgtacgcc gcttgctcaa cgccgtcgaa gaagccaagg ccgccgggtt
     5221 catcggcacc aacgtgctcg gctctggctt cgacatcgat gtcgtcgtgc acatcggcgc
     5281 cggtgcgtac atctgcggtg aagag
//
LOCUS       DASWVF010000306         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_313911, whole genome shotgun sequence.
ACCESSION   DASWVF010000306 DASWVF010000000
VERSION     DASWVF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313911"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="nadC"
                     /locus_tag="VGD55_05440"
     CDS             <1..871
                     /gene="nadC"
                     /locus_tag="VGD55_05440"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0869817.1"
                     /translation="LTHAGLDTAEVERLVAAALAEDLGGGVDVTSVAAVPFDHESIAD
                     FVSRAPGVIAGLPLVAVVLWTAAPDSAVDIDEHVVDGMRVDAGTALLSASGRTRTLLT
                     AERTALNLLGHLSGIATLTRAWVDAIAGSGAAIRDTRKTTPGLRALEKYAVRCGGGVN
                     HRMSLSDAALIKDNHVLAAGGVAPAFAAVRAAFPDVPVEVECDTVEQVGEAVDAGADL
                     VLLDNMSTQSMRAAVALVRERGSACRLEASGGLTLARAAEVAATGVDYLSVGALTHSA
                     PVLDIGVDLRPAL"
     gene            882..1649
                     /locus_tag="VGD55_05445"
     CDS             882..1649
                     /locus_tag="VGD55_05445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0869818.1"
                     /translation="MLLTIDVGNTQTVLGLFEGEVIVEHWRLATDPRRSADELGLLVI
                     GLLSASGHEDTIDGIACCSTVPSALREVRSMCERWFGAVTLVVVEPGIRTGVPVLYDN
                     PKEVGADRIVNTLAAFTLHGGPAIVVDFGTSTNFDVVSAQGEFLGGVLAPGIEISVDA
                     LASRAAQLMKVQLVRPRAVIGKNTVESLQSGIIYGFAAQVDGITERIIAELGTPATVI
                     ATGGLAPVVLEECRLIDVHEPWLTLSGLRLMFERNTG"
     gene            1682..3157
                     /gene="lysS"
                     /locus_tag="VGD55_05450"
     CDS             1682..3157
                     /gene="lysS"
                     /locus_tag="VGD55_05450"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0869819.1"
                     /translation="MTEDLPEQMLVRREKLDRLRAEGIDPYPVGFARTATIAEVRAEH
                     AGLPPDTMTGAHVGVVGRVVLSRNSGKLCFATIRDGSGELQVMASLDRLGEESLALWK
                     SAIDLGDHLGVEGEIITSRSGELSVLVDSWALTAKALRPLPDKHKGLADPEARVRQRY
                     VDLIVNSDARDMVRTRASVLQSVRDTMRRHGYVEVETPSLQTLHGGATARPFKTHLNA
                     FDLPLFLRIALELYLKRVVVGGIERVYEMGRVYRNEGVDSTHSPEFTELEAYEAYGDY
                     DTMAALTRDIVLDAANAVGRVVVPDGRGGEIDLAASWRSVTIHEVISQAVDQELTIDT
                     DVETLRAVAAKFDVSLQPDWDHGQVLLELFEKLVEHTLIEPTFVRDYPASVRPLARPH
                     RRDPRLVEAWDLVINGVELAPAYSELVDPVEQRRRLTEQSLLAAGGDPEAMQLDEDFL
                     RALEYGAPPMGGLGLGIDRLVMLLTGANIRETILFPLIRPE"
     gene            3161..3703
                     /locus_tag="VGD55_05455"
     CDS             3161..3703
                     /locus_tag="VGD55_05455"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY0869820.1"
                     /translation="MNQLPADITVRRARTADVNAIREFVDAYAPKRILLNKPTVTLYE
                     DVQEFWVAERALPVEGGAPPASVVVGCGALHVMWEDLAEVRTLAVAPAMRGKGIGHLL
                     LEHLLTTARELGVRRLFCLTFEVDFFASHGFAEIDGFPVAPDVFEELLRSYDEGVAEF
                     LDLERVKPNTLGNTRMIMYL"
     gene            3806..4153
                     /locus_tag="VGD55_05460"
     CDS             3806..4153
                     /locus_tag="VGD55_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEY0869821.1"
                     /translation="MAQKVQVTLVDDIDGSSADETLTFGIDGVFYEIDLSKSNAKKFR
                     ESLNAYVGAARRSGRSSRVGRPRGRAGRSKAAAGSAAPTSEIRAWARSKGIKVNERGR
                     IAADIVARYESSH"
     gene            4442..>4651
                     /locus_tag="VGD55_05465"
     CDS             4442..>4651
                     /locus_tag="VGD55_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0869822.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEGVRQQVEEIIGQG"
BASE COUNT          795 a         1417 c         1583 g          856 t
ORIGIN      
        1 gttgacgcac gccggcctcg acacggccga agtcgagcga ctggtcgcag cggcgctggc
       61 cgaggatctc ggtggtggcg tcgacgtgac gagcgtcgcc gcggtgccgt tcgaccacga
      121 atcgatcgca gatttcgtgt cgcgcgcgcc cggtgtcatt gccggtctgc cgctcgttgc
      181 cgtcgtgctc tggacagccg cacccgattc cgcagtcgac atcgacgagc acgtggtcga
      241 tgggatgcgc gtcgacgcag gcacggcgtt gctctcggcg tcgggccgca cccgcacatt
      301 gctcacggcc gaacgcacgg cgttgaacct gctggggcat ctgtcgggga tcgcgacgct
      361 gacgcgtgca tgggtcgatg cgatcgccgg cagcggcgcc gcgattcgcg acaccaggaa
      421 gacgacgccg gggctgcgcg cgctagagaa gtacgcggtg cgctgtggcg gcggcgtgaa
      481 ccaccgcatg tcgctgtctg atgccgcgct catcaaggac aaccacgtgc tcgcagcggg
      541 cggcgtcgcg ccagcgttcg cggcggtgcg ggctgcgttt cccgacgtcc cggtcgaggt
      601 cgaatgcgac accgtcgagc aggtgggcga agccgtcgac gctggagctg atctggtgct
      661 gctcgacaac atgtcaacgc agtcgatgcg ggcggcagtg gcgttggtgc gcgagcgcgg
      721 cagtgcgtgc cggctcgagg ccagcggcgg cctcacgttg gcgcgcgcag ccgaggtggc
      781 ggcaacgggt gtcgactatc tctccgtcgg cgcgttgacc cactccgcgc cggtgctcga
      841 catcggcgtc gatctgcgac cggccctatg aaggagcgca gatgctgctg acgatcgatg
      901 tcggcaacac ccaaaccgtg ctcggcctgt tcgaaggtga agtcatcgtc gagcactggc
      961 ggctagcaac cgacccgcgc cggtctgcag acgagctcgg gttgctggtc atcgggttgc
     1021 tctcggcgtc gggacacgaa gacacgatcg acggcatcgc gtgctgctcg acggttccgt
     1081 cggcgttgcg cgaagtgcgg tcgatgtgcg agcgctggtt cggtgcggtc acgttggttg
     1141 tcgtcgagcc gggcatccgc accggcgtgc cagtgctgta cgacaacccc aaggaggtcg
     1201 gggccgaccg cattgtcaac accctcgccg cttttacttt gcacggtggt ccggccatcg
     1261 tcgtcgattt cgggacgtcg accaacttcg acgtcgtctc ggcacagggc gaattccttg
     1321 ggggagtgct cgcgcccggc atcgagatat cagtcgatgc gctggcctcg cgtgccgcac
     1381 agctcatgaa ggtccagctc gtgcggccgc gagcggtgat cgggaagaac acggtcgagt
     1441 cgctccagtc gggcatcatc tatggcttcg ctgcgcaggt cgacgggatc accgagcgca
     1501 tcatcgccga acttggcacc ccggcgaccg ttattgccac cggcgggctc gcacctgtgg
     1561 tgttggagga atgccggctc atcgacgtcc acgagccgtg gttgaccttg agcgggttgc
     1621 ggctgatgtt cgaacgcaat accggatgac ccacgaaaga gcggccgata gccttccggc
     1681 cgtgaccgaa gatttgcccg agcagatgct cgtacgccgc gagaagcttg accggctgcg
     1741 ggccgagggc atcgacccgt acccggtggg ctttgcgcga accgcgacga tcgctgaggt
     1801 tcgggccgaa catgcaggtc tgccgcccga cacgatgacc ggtgcccacg tcggggtcgt
     1861 cggccgtgtc gtcttgtcgc gcaactcagg caagctgtgc ttcgcgacga tccgcgacgg
     1921 ttctggagag cttcaggtca tggcgtcgct ggatcgcctc ggcgaggagt cgctggcgct
     1981 gtggaagtcg gcgatcgatc tcggtgacca tctcggcgtc gagggcgaga tcatcacgtc
     2041 gcgcagcggt gagctgtcag tgcttgtcga ttcgtgggcg ctgaccgcca aggcgttgcg
     2101 accactgccc gacaagcaca agggcctcgc cgatcccgag gcgcgggtgc gccagcggta
     2161 cgtcgacctg atcgtgaact cagatgcacg cgacatggtg cgtacgcgcg ccagcgtctt
     2221 gcaatcggtg cgcgacacga tgcgtcgcca cggctacgtc gaggtcgaga cgccgtcgct
     2281 gcagacgctg cacggcggtg ctaccgcgcg gccgttcaag acccatctca atgcgttcga
     2341 cctgcccttg ttcctgcgca tcgcgctcga gctgtacttg aagcgggtcg tggtcggcgg
     2401 catcgagcgg gtctacgaga tgggccgcgt gtaccgcaac gagggcgtcg actcgaccca
     2461 cagccccgag ttcaccgaac tcgaggcata cgaggcctac ggcgactacg acacgatggc
     2521 cgccttgacg cgcgacatcg tgctcgatgc tgcaaacgcc gtcggtcggg tcgtggtgcc
     2581 agatggccgg ggtggtgaga tcgacctggc cgcgtcgtgg cgatcggtca cgatccacga
     2641 ggtgatttcg caagccgtcg atcaggaact cacgatcgat accgacgtcg aaaccctgcg
     2701 agccgttgcc gcgaaattcg atgtgtcgtt gcagcccgat tgggatcacg ggcaggtgct
     2761 gctcgagttg ttcgaaaagc tcgtcgagca cacattgatc gagcccacat tcgtgcgtga
     2821 ctatccggcc tcggtacgac cattggcccg gccacatcgg cgcgacccgc ggctcgtcga
     2881 ggcctgggat ctcgtcatca acggggtcga actcgcgccc gcctattcgg aacttgtcga
     2941 cccggtcgag caacgtcgcc gcctcaccga gcagtcgctc cttgcagcgg gcggcgatcc
     3001 cgaagcgatg caactggacg aggatttcct aagagcgctc gaatatggcg cgccgcctat
     3061 gggtggcctc ggtcttggaa tcgaccggct cgtgatgttg ctcaccggcg caaacatccg
     3121 agaaacgatc ctgttcccat tgatcaggcc cgagtaagcg atgaaccaac tccctgcgga
     3181 catcacggtg cggcgtgccc gcacggcgga cgtgaatgcg attcgcgaat tcgtcgacgc
     3241 ctatgcgcct aagcgcatat tgctcaacaa accgacagtg accctgtatg aagatgtaca
     3301 ggaattctgg gtcgccgagc gcgcgctgcc cgtcgaaggt ggcgcgcccc ccgcgtcggt
     3361 tgtggttggc tgcggggctc tgcacgtgat gtgggaagac ctcgccgaag tgcgcaccct
     3421 cgcggtcgcc ccggcaatgc gcgggaaagg catcgggcat ttgcttctcg agcatttgtt
     3481 gacgacggcc cgcgagctcg gcgtccgccg gctgttttgc ctgacgttcg aagttgattt
     3541 ctttgcctcg cacggtttcg ccgagatcga tgggtttccg gtcgcgcccg acgtgttcga
     3601 agagttgctc agatcgtacg acgaaggcgt tgcggagttt ctcgaccttg aacgggtgaa
     3661 gccgaacact ctcggtaaca cccgcatgat catgtacttg tgaactacac gatcgggccg
     3721 gtgcgcagct atttgcattc gccgcgcatt tccgatttgc tttccgcaat cgagtcatgc
     3781 gcatgaagtt gaaaggacgc ctcccgtggc acaaaaggtc caggtaacgc tcgtcgatga
     3841 catcgacggc agttcggcag acgaaaccct cacgtttggc atagatggcg ttttctacga
     3901 gatcgacctg agcaagagca atgcgaagaa gttccgggag tcgctgaatg cgtatgtcgg
     3961 cgcggcgcgc cggtctggtc gctcgtcgcg ggtgggtcgt ccgaggggcc gcgccggtcg
     4021 gtcgaaggcg gcagcaggta gcgcggcgcc gacgtcggag atcagggcgt gggcacggtc
     4081 caagggcatc aaggtaaacg agcgcggccg gatcgcggcc gacatcgtcg cgcggtacga
     4141 gtcctcccac tagcgctgct gttcgctcgg agcgcaacgc tcacggaaca ggcacggctg
     4201 ggtcacggtt tggagtctta ggacgctggg gaccgatttt cgggttctat cgcccgaatc
     4261 ggacgtattt ttggtcctga cctcggaaaa gttggcctga agggcgcaag tctcgccgtg
     4321 tcttggcgag aactgtcgca agcaggcgtg gccggggtgg ggcagcgcca ggtggctatt
     4381 gggcaggtgc gtcccggagc gcgggtagca ttaaagaagt aggcgcagag gagcgagcga
     4441 catgttcgag agatttaccg accgagcccg acgtgttgtc gtgctggcgc aagaagaagc
     4501 ccgcatgctc aaccacaact acatcggcac cgagcacatc ctgctcgggc tcatccacga
     4561 gggcgaaggt gtcgccgcga aggctcttga gagtctcggt atttcgcttg aaggcgttcg
     4621 ccagcaggtc gaagagatca tcggacaagg c
//
LOCUS       DASWVF010000307         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_441715, whole genome shotgun sequence.
ACCESSION   DASWVF010000307 DASWVF010000000
VERSION     DASWVF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441715"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="mqnP"
                     /locus_tag="VGD55_05470"
     CDS             <1..966
                     /gene="mqnP"
                     /locus_tag="VGD55_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis prenyltransferase MqnP"
                     /protein_id="HEY0869823.1"
                     /translation="RPSGHRPGNGALEGVRHLVSATAASLTPAARAPGRVRAFLDLVR
                     FEHSVFALPFAYISALTAMSAVNHRVHWRQLALVTIAMVGARTFAMAVNRIVDRHIDA
                     RNPRTKGRELVTGAVSLRTAWVGAIVALAVLMAAAAGLNRLCFVLAPVAAIVLAVYPY
                     AKRFTWAPHAVLGLAQTIGPIGAWMAVTGRWSWTAVVLGLAVGCWIGGFDIIYALQDY
                     EVDRRIGVKSLPARFGPHVALAFSRLAHYATVLLLLWFGALAHLGWTWAAGVVLAAGA
                     LAYEQRLVKPDDLSKVNRAFFTVNGFVGIGLFCFALADLVSRGLR"
     gene            963..1667
                     /locus_tag="VGD55_05475"
     CDS             963..1667
                     /locus_tag="VGD55_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY0869824.1"
                     /translation="MTTGRVPWVVGVTGASGTPYAAAVLRGLLAASLPVDLVVSRAAR
                     LTLLDETGIAWHDDADSLRAWLTSHLDSDLPPLVDDAVLDHVRFWSAGDLAAGPSSGS
                     YRTRGMVVVPASTAAVAGIAIGLSKDLLQRAADVTLKERRPLVVVPREMPFTGATLRH
                     LATLDAAGATVAPASPAFYGKPKNMQQLVDFVAGKILDALDVEHDLFTRWDGRLGMGV
                     AGGNGGNGGAIDARDR"
     gene            complement(1671..1922)
                     /locus_tag="VGD55_05480"
     CDS             complement(1671..1922)
                     /locus_tag="VGD55_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEY0869825.1"
                     /translation="MPLSQRKAHLVENRDRLLTPGEVASLFRVDPKTVTRWAASGRIG
                     SIRTPGGHRRFRESEVRALLRGDSDLDSIGSLALEASEG"
     gene            2268..3416
                     /gene="mqnE"
                     /locus_tag="VGD55_05485"
     CDS             2268..3416
                     /gene="mqnE"
                     /locus_tag="VGD55_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY0869826.1"
                     /translation="MDSGRKRELESKVLDGERLTYDDGVALYASDDLAWLGELAHHVR
                     TVKNGDRTYFNVNRHLNLTNVCAASCAYCSFQRKPGEADAYTMRIEQAVELAKQMEPE
                     GITELHIVNGLHPTLPWRYYPRSLKALKDALPGVALKAFTATEIHWFEEISGLSADEI
                     LDELIDAGLESLTGGGAEIFDWEVREKIVDHKTHWEDWSRIHRLAHSKGLKTPCTMLY
                     GHIEEPRHRVDHVLRLRELQDETGGFVVFIPLRFQHDASGDPRNRLMTQPMATGAEAL
                     KTFAVSRLLFDNVPHVKAFWVMHGLTTSQLALSHGADDLDGSVVEYKITHDADHFGTP
                     NTMHRDDLLELIRDAGFTPVERDTRYNVLRVFDGPDKTRRDAPQAMPV"
     gene            3427..3801
                     /locus_tag="VGD55_05490"
     CDS             3427..3801
                     /locus_tag="VGD55_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEY0869827.1"
                     /translation="MTEDAGKPARSHPFIRYTSARIALFVIAVVVVWLVRLASGVLLV
                     IVALLISGLASFALLGRQRAEVSAALFERRERQRAHRWAAYNDDDENSDENGESNHEQ
                     DGLQALQSGYPESDRARTERPS"
     gene            3831..>4084
                     /locus_tag="VGD55_05495"
     CDS             3831..>4084
                     /locus_tag="VGD55_05495"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0869828.1"
                     /translation="MPEPGLREAGAEHVQLRTGQLSWREFDGETVLLDLAGSKYLTVN
                     AAGTVLLKHLVGGCTRDDLVTALVDAFGIPTAQAAADVEA"
BASE COUNT          659 a         1310 c         1368 g          747 t
ORIGIN      
        1 agacccagcg gtcatcgccc gggtaacggc gcgctggaag gagttcggca tctcgtgagt
       61 gcgactgctg cctccttgac gcccgctgcc agagcgcccg gccgggtgcg tgcgttcctc
      121 gacctcgtgc ggttcgagca ctcggtgttc gcgctgccgt tcgcctacat ctccgcactc
      181 accgcgatgt cggccgtcaa tcaccgcgtg cactggcgtc aactcgcgtt ggtcacgatc
      241 gcgatggtcg gagcccgcac attcgcgatg gcggtcaacc gcatcgttga tcggcacatc
      301 gacgcgcgca acccgcggac gaagggccgc gagctcgtca cgggcgcagt gagcttgcgc
      361 acggcgtggg tgggcgcgat cgtcgcactg gccgttctca tggcggcggc cgccggactg
      421 aaccgcctct gtttcgtgct cgcgcctgtg gcggcgatcg tcttggccgt ctacccgtac
      481 gcgaagcgct tcacctgggc accgcacgcc gtgctaggtc tcgcccagac gatcgggccg
      541 atcggtgcat ggatggctgt caccggccgc tggtcgtgga cagcggtcgt tctcgggctc
      601 gccgtcggct gctggatcgg cggcttcgac atcatctacg cgctccagga ctacgaggtc
      661 gaccggcgga tcggcgtgaa gtcgctcccc gcgcggttcg gcccgcacgt cgcgctcgcg
      721 ttctcgcggc ttgctcacta cgcgaccgtg ttgctgttgc tgtggttcgg cgctctcgca
      781 cacctcggct ggacgtgggc ggcgggcgtc gtgctggccg cgggtgcgct cgcgtatgag
      841 cagcgactcg tgaaacccga cgacttgtcg aaggtcaacc gggcgttctt cacggttaac
      901 ggcttcgtcg gcatcgggtt gttctgcttc gcactcgccg acctcgtatc gcgaggcctt
      961 cggtgacgac gggccgcgtg ccatgggtcg tgggcgtgac cggcgcgagc ggaacccctt
     1021 atgcggcagc ggttttgcgc ggtctgctcg ctgcatcgct gccggtcgac ctcgtagtct
     1081 cacgggctgc acggctcacg ctcctcgacg aaaccggcat cgcgtggcac gacgatgccg
     1141 acagcctgcg agcgtggctc acgtcgcacc ttgattctga tctgccgcca ctcgtcgacg
     1201 acgccgtgct tgatcacgtg cggttctggt cggccggtga tcttgcggcc ggcccttcga
     1261 gtgggtcgta ccgcacccgt ggcatggtcg tcgtgccggc gagtacggcc gctgtcgcgg
     1321 gcatcgccat tgggttgtcg aaggatctgc tccagcgcgc cgctgacgtg acgctgaagg
     1381 agcggcggcc actggtcgtc gtcccgcggg aaatgccctt caccggcgcg acgctgcgcc
     1441 acctggccac cctcgacgct gccggcgcaa ccgtggcccc tgccagcccg gcgttctacg
     1501 gcaagccgaa aaacatgcag caactcgtcg acttcgttgc cggcaagatc ctcgacgcgc
     1561 ttgacgtcga gcacgacctc ttcacgcgtt gggacggccg cctgggtatg ggagtcgccg
     1621 gcggtaacgg gggcaacggc ggagcgatcg acgcgcgcga tcgctgagaa tcagccttcg
     1681 gacgcctcga gtgcgagcga cccgatcgag tcgagatcgg agtcgccgcg cagcaaggcg
     1741 cggacttcgg attcccggaa gcgacgatgc ccaccgggtg tgcgaatgct gccgatccgg
     1801 ccggaagctg cccaacgtgt cacggtcttt gggtcgacgc gaaacaagct ggcaacctct
     1861 ccgggtgtca ggagccggtc acggttctcc acgagatgtg cctttctttg ggacaacggc
     1921 attactggta aacggtgttc tggcgtgtgc tgagctgatc cttggacaca ctgtcgttta
     1981 acgcttttgc tggcccccag aagatgtgta cacgcgttgt gtggtctcgt ctgcctttgg
     2041 cgcacgttag ttgcgtagcg gacaaaaaag tcggggtggt cgtcacgaac cggtcaccgt
     2101 gagtgaccga aaccgccaat aaaggtcccg gatggggcta agagtcgcca gatgatcgct
     2161 gtgcgtgagc gctcgtatga gcttgatcgc gggccggtgc aacgcttcgt tgcgcccgct
     2221 ccggacccgc gcgtgcgccg cgatcgacaa ccgtgcatgc ttgaaacgtg gacagcggcc
     2281 gtaagcgtga actcgaatcc aaggtgttgg acggcgaacg actgacgtat gacgacggcg
     2341 tggcgctgta cgcgagcgat gacctcgcct ggctcggtga gctcgcccac cacgtacgca
     2401 cggtgaagaa cggcgatcgc acctacttca atgtcaacag gcatttgaac ctgacgaacg
     2461 tgtgcgcggc gtcgtgcgcg tactgcagtt tccagcgcaa gcctggtgag gccgacgcgt
     2521 acacgatgcg catcgagcaa gcggtcgagc ttgccaagca gatggagccc gagggcatca
     2581 ccgagctgca catcgtgaac ggcctgcacc cgacgttgcc gtggcgctac tacccgcggt
     2641 cgcttaaggc cctgaaagac gcccttcccg gcgtcgcact gaaggcgttc accgccaccg
     2701 agatccattg gttcgaggag atctcggggc tgtcggctga cgagattctc gacgagctca
     2761 tcgacgccgg acttgagtcg ctgaccggtg gcggtgctga gatcttcgac tgggaggtgc
     2821 gggagaagat cgtcgaccac aagacgcact gggaagactg gtcccgcatt caccggctcg
     2881 cgcacagcaa gggcctgaag acgccgtgca cgatgctgta cgggcacatc gaggagccgc
     2941 gccaccgtgt cgaccacgtg cttcggttgc gcgagctgca agacgagacg ggcggcttcg
     3001 tcgtgttcat cccgctgcgg ttccagcacg acgcgagcgg tgacccacgc aaccggctca
     3061 tgacccagcc aatggcgact ggcgctgagg ctctgaagac gttcgcggtg tcgcggctgt
     3121 tgttcgacaa cgtgccgcac gtgaaggcgt tctgggtgat gcacggcttg acaacgtcgc
     3181 agctggcgct ctcgcacggc gcagacgacc tcgacggttc ggttgtcgaa tacaagatca
     3241 cccacgacgc tgaccacttc ggaactccga acacgatgca tcgcgacgac ctgctcgagc
     3301 tcatccgcga cgctgggttc acgcccgtcg agcgcgatac gcgttacaac gtgctgcggg
     3361 tgttcgacgg gccggataag acccgacgcg acgccccgca agcgatgcca gtttgagttg
     3421 cgcgtgatga ctgaagacgc aggcaagccc gcgcggtctc acccgttcat ccggtacacc
     3481 tccgcgcgga tcgcgctgtt cgtcatcgcg gtcgtggtgg tctggctggt gcggctggcg
     3541 agcggcgtgt tgctggtgat cgtcgcgctg cttatctccg gattggcgag ttttgccttg
     3601 ctcgggcgcc agcgcgccga ggtatcggcg gcgctgttcg agcgccgcga gcgtcagcgc
     3661 gcgcaccgat gggcggccta taacgacgac gacgagaaca gcgacgagaa cggtgaaagt
     3721 aaccatgagc aggacgggct gcaggccctg caatccggct accctgagtc cgatcgcgca
     3781 cgtaccgaac gcccctcgta gggattcgca aggcaggagc ggatgtctgc atgccagagc
     3841 cgggtctccg cgaggcaggg gcggagcacg tgcagctacg cactggccag ctgagttggc
     3901 gcgagttcga cggtgagacc gtgctccttg acctcgcggg ttccaagtac ctgacggtca
     3961 acgcagccgg aaccgtgctc ctcaagcacc tcgttggcgg ctgcacgcgt gacgatctcg
     4021 taaccgcgct tgtcgacgcc ttcggcattc ccaccgccca ggccgccgcg gacgtcgagg
     4081 cgtt
//
LOCUS       DASWVF010000308         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_110360, whole genome shotgun sequence.
ACCESSION   DASWVF010000308 DASWVF010000000
VERSION     DASWVF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110360"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..444)
                     /locus_tag="VGD55_05500"
     CDS             complement(43..444)
                     /locus_tag="VGD55_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869829.1"
                     /translation="MVDGWFRTGDLGRVGDDGWAYIIDRVKDVIISGGENIYPAEVEA
                     VLAQLPQVAECAVVGVPDEKWGEVGLAYIAARSDALLDEATVRVHLEKRLAKFKRPHY
                     IRFVETLPRTATGKLMKAALRADFAQTSEPK"
     gene            complement(484..1518)
                     /locus_tag="VGD55_05505"
                     /pseudo
     CDS             complement(484..1518)
                     /locus_tag="VGD55_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(1571..>2289)
                     /locus_tag="VGD55_05510"
     CDS             complement(1571..>2289)
                     /locus_tag="VGD55_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0869830.1"
                     /translation="EIAQYYRERQLAAVIFTIDAHTATGHDALSSESIVEQAAMHADV
                     LIPFCSVDPHGGPSAVSRLRDLVAAGARGLKLHPSLQAFSPNAPNFYPLYETAAALGV
                     PIIFHTGQTGIGAGLPGGRGIKLRYSDPMLIDDIAADFPTLSIVLAHPSVPWQDASIS
                     IATHKANVMIDLSGWSPTYFPPQLVRAVNGLLKHKMMFGSDFPLITPDRWLKDFDALD
                     IKPDVRPLILKDNAARWLGL"
BASE COUNT          405 a          731 c          761 g          392 t
ORIGIN      
        1 cgacaagagg cttgaggtcg gcgatggtgt cgatcgtggt ggtcatttgg gctccgatgt
       61 ctgcgcgaag tcggctcgca gcgcggcctt catcagcttg ccagtagcag tacgtggcaa
      121 cgtttcgacg aaccggatgt agtgcggtcg cttgaacttc gcgagccttt tctccagatg
      181 cacccgcacg gtcgcctcgt cgagaagcgc atcgctgcgg gccgcgatgt aggcgagtcc
      241 gacctcgccc cacttttcat caggtacgcc gacgactgcg cactcggcga cctggggaag
      301 ctgtgccagc acagcttcga cctcggcggg gtagatgttc tcaccgcccg agatgatgac
      361 gtccttcacg cggtcgatga tgtaggccca gccgtcgtcg ccgacccgcc caagatcgcc
      421 ggtgcgaaac cagccgtcga ccatgacgtc ggccgtttcc ttcgggcgtt gccagtagcc
      481 ggcgaagacg ttcggtccgg tgacgagcac ttctccggtg ccggggggaa ccgcgacgat
      541 gccgtcgctg ccgagcaacg cgacgtccgt gaagaaatgc tgcacccctg ctgacgtcgg
      601 ccgctgcgcc gacccctcgg gcagcgatag aaagactccc ggcgcggcct ctgtcatgcc
      661 gtacccctgc aagacgtcga cgccgcgatc aagccaatcc gtcgcgacgc gttcgagcac
      721 cggtgagccg ccatagatca cgtagcgcag cgacgacagg tcggcatcgc gtcagtcggc
      781 gtggtcgcac agcatctgca tcatcgtggg cacagccgag aacgcggtga tgcgcagggc
      841 ggcgatagtg gccagcatcc acgccggatc gaacttcggc gtcagcacga ccgtgccgcc
      901 cttgaagagt gtaggcaacg tcacctggcc gagcccgacg gcgtggaaga gcggcgcggt
      961 gcacaacacg atgtcgctac cgagcacgtc gacgtgggcg agctggttca tggtgttgaa
     1021 cgtcagattg ccgtgggtga gcacggctcc ctttggtcga cccgtcgtac cactcgtgta
     1081 gaggatcagt gcggggtcgt cgagagagac ctcggtctcg ctgaaaccga cctcgctcaa
     1141 accggcggag gtaagaccgt cgatgtgagc tgcgctcacc accgtctgca cgccgcacgc
     1201 gatcgggtcg gcttcggcga ccagcgacgc cgcttccggc ccgtgcacca gcacacgagc
     1261 tccggaatct ctgagcaggt cggcaatttc cgcgcccgcg agtcgggtgt tcaggggcac
     1321 gaagatcgcg ccgagacgac cggtcgcgaa caacgtctcg aacgtcgcga tgtcgttgac
     1381 tccgaggtac gcgacgcgat cgcccttggc gacgcccaac gacgcgagcc cgccggccaa
     1441 tgcattgaca cggtcggcaa gttgccggta cgtcaacgtc gcatcgcctt gccgcagcgc
     1501 taccgcatcg ggtgccatgc gggcgcgtcg caacggccag ctaccgatcc cttgatcgct
     1561 cacagcgcgc ctagagtccg agccagcggg cagcgttgtc tttcaagatc agcggacgca
     1621 cgtcgggctt gatatcgaga gcgtcgaagt ccttgagcca gcggtcaggg gtgatcagcg
     1681 ggaagtccga cccgaacatc atcttgtgct tgagcaggcc gttcactgct cgcaccagtt
     1741 gcggcgggaa gtacgtcggc gaccaacctg acaggtcgat catgacgttg gccttgtgcg
     1801 tcgcgatcga gatcgaggcg tcctgccacg gcaccgacgg gtgcgcgagc acgatcgaca
     1861 gcgtcgggaa gtcggcggcg atgtcgtcga tcagcatcgg gtctgaatac cgcagcttga
     1921 tgccacggcc acctggcagg cccgcgccga ttccggtttg ccccgtatga aagatgatcg
     1981 ggacgccgag ggcggccgcc gtctcgtaca gcgggtagaa gttcggcgca ttcgggctga
     2041 acgcctgaag cgaggggtgc agcttcagcc cgcgggcgcc ggcagcgacc agatcgcgca
     2101 ggcgacttac agcgctcggg ccgccgtgcg ggtcgaccga gcagaacggg atcagcacgt
     2161 cggcgtgcat ggcggcctgc tcgacgatgc tctcactcga cagcgcatcg tgaccggttg
     2221 cggtgtgcgc gtcgatggtg aagatcactg ctgcgagttg ccgttcgcgg taatactgcg
     2281 cgatctctt
//
LOCUS       DASWVF010000309         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_161093, whole genome shotgun sequence.
ACCESSION   DASWVF010000309 DASWVF010000000
VERSION     DASWVF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161093"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1802
                     /gene="glgB"
                     /locus_tag="VGD55_05515"
     CDS             16..>1802
                     /gene="glgB"
                     /locus_tag="VGD55_05515"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0869831.1"
                     /translation="MKKATSVPTAPDAQAGSPAPAAPDAQAGSPAPAAPSAPPAPSAP
                     SVPAEPAPPPAPVTAPPADLDRLVSGAHHNPHELLGAHLREGVVTFRSLRPNAREVTV
                     LIGAARVPLMHERGGVWSGSWQSATVPDYRIEIVYDDGIPHIVDEPYRFLPTLGDIDL
                     HLIGEGRHEQLWEVLGAHEHQYDTPSGVVHGTSFAVWAPNARAMRVAGDFNFWDSQAF
                     PMRSLGSSGVWELFIPGVGAGAQYKLNVLGSDGQWREKADPMAFATEHPPAQASVVFT
                     STYEWRDAEWLAKRAHADALREPMSIYEVHLGSWKQGLGYRQLADELVAYVKDLGFTH
                     VELLPVAEHPFGGSWGYQVSSYFAPTSRFGNPDDFRFLVDNLHQAGIGVIIDWVPAHF
                     PKDAWALAQFDGTHLYEHSDPQLGEHPEWGTLVFNFGRREVRNFLVSNAVYWAEQMHI
                     DGLRVDAVASMLYLDYSRKAGEWRPNVYGGRENLEAVAFLQEMNATIYRRAPGVVTIA
                     EESTAWPAVSKPTYVGGLGFGFKWNMGWMHDSLQYISHPPIYRSYHHNEMTFSMMYAY
                     TENFVLPISHDEVVHGKGSLLHKMPGDRW"
BASE COUNT          316 a          625 c          567 g          294 t
ORIGIN      
        1 gggaaggcac ccgcagtgaa gaaggcaaca agcgtgccga ccgcgccaga cgctcaagcc
       61 gggtcaccgg caccagccgc accagacgct caagccgggt caccggcacc agccgcccca
      121 tcggcgccac cggcgccgtc cgcgccctcc gtaccagccg agcccgcccc gccaccggca
      181 cccgtcaccg cacctccggc cgatctcgat cggctggtat cgggcgcgca tcacaatccg
      241 cacgagctcc tcggcgcgca cctgcgcgaa ggcgtcgtca cgttccgcag ccttcgaccg
      301 aacgcgcgcg aggtcacggt gctcatcggt gccgcgcgcg tcccgctgat gcatgagcgc
      361 ggcggagtgt ggagcggctc gtggcagagc gcgaccgtcc ccgactaccg catcgagatc
      421 gtttacgacg acggcattcc gcacatcgtc gacgagccgt accgcttcct gccgacgctc
      481 ggcgatatcg acctgcacct gatcggtgag ggccggcacg agcagttgtg ggaagtgctc
      541 ggtgcccacg agcaccagta cgacaccccg agcggcgttg tgcacggcac gtcgttcgca
      601 gtctgggcgc cgaacgcgcg cgccatgcgc gtcgccggcg acttcaactt ctgggactcc
      661 caagcgttcc cgatgcgatc actcggctcg agcggcgtct gggagttgtt catcccaggc
      721 gtcggcgccg gtgcgcagta caagctcaac gtcctcggat ccgatggcca gtggcgtgaa
      781 aaggccgacc cgatggcctt cgcgaccgaa catccgccgg cgcaggcatc cgtggtgttc
      841 acgtcgacat acgaatggcg ggacgccgag tggctcgcga agcgggcaca cgccgacgcg
      901 ctgcgcgaac cgatgagcat ctacgaggta cacctcggct catggaagca gggccttggc
      961 taccgccagc tcgccgacga gcttgttgcg tacgtgaagg atctcggctt cacccacgtc
     1021 gagttgctgc cggtcgccga acacccattc ggcggctcgt ggggctatca ggtttcgtcg
     1081 tacttcgcgc ccacgtcgcg cttcggcaac cccgacgact tccgcttcct cgtcgacaat
     1141 ctgcaccagg ccggcatcgg cgtcatcatc gactgggtcc ctgcgcactt cccgaaggat
     1201 gcgtgggcgc tcgcccagtt cgacggcacg cacctgtacg agcactcgga cccccagctc
     1261 ggcgagcacc ccgaatgggg gacgctggtc ttcaacttcg gccgccgcga ggtgcgcaac
     1321 ttccttgtct cgaacgccgt gtactgggcc gagcagatgc acatcgacgg attgcgagtc
     1381 gacgcggtgg cgtcgatgtt gtatctcgac tactcgcgca aagccggcga gtggcgaccg
     1441 aacgtctatg gcgggcgcga gaacctcgag gcggtcgcgt tcttgcagga gatgaacgcg
     1501 acgatctatc gacgcgcacc tggtgtcgtg acgatcgcag aggagtcgac cgcctggccg
     1561 gccgtctcga agccgacgta cgtcggcggt ctcggcttcg gcttcaaatg gaacatgggt
     1621 tggatgcacg actcactgca gtacatcagc catccgccca tctaccgcag ctatcaccac
     1681 aacgagatga cgttctcgat gatgtacgcc tacaccgaga acttcgtgct gccgatctcg
     1741 cacgacgagg tcgtgcacgg caagggttcg ttgttgcaca agatgcccgg agaccgctgg
     1801 ca
//
LOCUS       DASWVF010000310         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_136026, whole genome shotgun sequence.
ACCESSION   DASWVF010000310 DASWVF010000000
VERSION     DASWVF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136026"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VGD55_05520"
     CDS             <1..1066
                     /locus_tag="VGD55_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0869832.1"
                     /translation="DTIGAMVDAGLKASGFQEVGLLSLSSADHSEIGPLAKQLADRYE
                     GTNTSLSLPSTRVDAFNVDLANEFSRNGRRSGLTFAPEGGSERMRKVINKMVTEEDLI
                     RTVTTAYAQGWRRVKLYFMCGLPTETDDDVLQIADLARKVIQAGRTASGSRDIGCTVS
                     IGGFVPKPHTPFQWAAQLDHETTDTRLLKLRDTIRTDRQYGKAIGFRYHDGKPGIVEG
                     LLSRGDRRVGRVIKQVWLDGGRFDGWSEYFSFDRWMRAADTALAGEPVDVDWYTTRER
                     DRSEVLPWDHLDSGLDKDWLWQDWQDSLSDVEVEDCRWTPCFDCGVCDTMGTDIQVGP
                     TGRTLLPLTVLGAASAPQPV"
     gene            1068..>1620
                     /locus_tag="VGD55_05525"
     CDS             1068..>1620
                     /locus_tag="VGD55_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEY0869833.1"
                     /translation="MARQPDAREVAPAVQRLRIHFAKRGRLRFTSHRDFQRVFERAVR
                     RAEIPVAFSAGFSPHPKISYVGAASTGAASEAEYLEIAVTQMCDPRRVRAELDAALPP
                     GFDVIEVLESRGGSLPDRIDASHWQVTLNGVDPATALRAVEAFLQAESIPVERVLKDG
                     RRMLDARLPVVHLGVVDDPGPTGC"
BASE COUNT          273 a          544 c          547 g          256 t
ORIGIN      
        1 cgacacgatc ggtgcgatgg tcgacgccgg cctgaaggcg agcgggttcc aagaagttgg
       61 cttgctgtcg ctcagcagcg ccgaccattc cgaaatcggc ccgctcgcca agcaactcgc
      121 tgaccgctac gaaggaacca acacgtcgct ttcgctgcct tcgacgcgcg tcgatgcgtt
      181 caacgtcgat ctcgcgaacg agttctcccg caacggtcgc cgatcggggc tcacgttcgc
      241 gcccgagggc ggcagcgagc gtatgcgcaa ggtcatcaac aagatggtca ccgaagaaga
      301 cctgatccgc acggtgacga ccgcctacgc ccaaggctgg cggcgcgtga agctctattt
      361 catgtgcgga ttgccgaccg agaccgacga cgatgtgctc cagatcgccg acctggcacg
      421 caaagtgatt caggcaggcc ggacggcgag tggcagccgc gacatcggct gcaccgtttc
      481 gatcggcgga ttcgtgccca agccgcacac cccgttccag tgggcggcgc aactcgacca
      541 cgagaccacc gacacccgac tactgaagtt gcgcgacacg attcgcaccg accggcagta
      601 cgggaaggcc atcggattcc gttaccacga tgggaaaccg ggcattgtgg aagggttgct
      661 ctcgcgcggt gaccgtcggg tcgggcgcgt catcaagcag gtgtggctcg acggcggccg
      721 cttcgacggc tggagtgagt acttctcctt cgacagatgg atgcgcgcag ccgacaccgc
      781 tctcgcgggc gaacccgtcg acgtcgactg gtacacgacg cgcgagcgcg accgctccga
      841 ggtgctgccg tgggaccacc tcgactcggg cctcgacaag gattggctct ggcaggactg
      901 gcaggactcc ctctccgacg tcgaggtcga agactgccgc tggacgccgt gcttcgactg
      961 cggcgtctgc gacacgatgg gcaccgacat ccaggtcggg ccgaccggtc gcacgctgtt
     1021 gccgctgacg gtgctcggcg cagcatccgc cccgcagccg gtctgacgtg gcccgccagc
     1081 ccgacgcacg cgaggtagcc cccgccgtcc agcgattgcg cattcatttt gcgaagcgtg
     1141 ggcggcttag gttcaccagt caccgcgact tccagcgggt gttcgagcga gccgtgcggc
     1201 gggccgagat tccggtcgcg ttcagcgccg ggttctcacc gcacccgaag atctcctacg
     1261 tcggcgcggc gtcgaccggg gcggccagtg aggcggagta cctcgagatc gcggtcaccc
     1321 agatgtgcga cccgcggcgg gtgcgcgccg agctcgacgc cgcgctgccg ccggggttcg
     1381 acgtcatcga ggtgctcgag tcgcgagggg gatcgctgcc cgaccggatc gatgcgtcgc
     1441 actggcaagt cacgctcaac ggggtcgacc cggcgaccgc tttgcgcgcg gttgaggctt
     1501 tcctacaggc cgagtcgatc ccggtcgaaa gggtcctcaa ggacgggcgc cggatgctcg
     1561 acgcccgcct gccggtagtc cacctcggcg tggtcgacga cccgggaccg acaggctgtg
//
LOCUS       DASWVF010000311         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_212478, whole genome shotgun sequence.
ACCESSION   DASWVF010000311 DASWVF010000000
VERSION     DASWVF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212478"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="VGD55_05530"
     CDS             complement(<1..1611)
                     /locus_tag="VGD55_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869834.1"
                     /translation="MKTTGTKLFCMTLAAAASGAVFNAHPARACNTGQTMSYYGGYVD
                     AYDQPGNIYYVFWGYGTYGDPQSFESTALAIYNAVAPLESLGIGATRFDGVTTQYAGL
                     DWNSSAFEQIAPTRDGFWFFDESGLPAPDPVTGQITLSDTDIMNEADFVASNVSASYD
                     DIFVIFTPSNAPPPVAGLCGQHFVSTQDTITAWVSYPGQGGCGFAFQQGAIMHEITEA
                     TANPAWNDGANFEGWDQGTGAVCEIGDLCNGIHYTIQTQPASNSPSQITTQLEFSNEA
                     VSAVGNGCVYGRSTKAYVAGLTSSAHLGTTTVAANTSVAFTATIDWGTPSGVTLTGTP
                     AVASWGSGHVDAFARATNGKMYHASSDDEGVTVTWELLTYPNSFTQSPDAVSWGGGNV
                     QVFAVSGSSVLKNTKNYGASWSGWTTVNLPSGVSPASKVSVASWGATNTSGGFPTNVR
                     TVVAAFRATSGNMYVGNSVAGGSFTWTNAGHPASLTGDPDLSAWAPPRFDLFVLDTSG
                     NFWDMRSTNGTSVAGSTNFGHPTSGGFQV"
     gene            1717..1962
                     /locus_tag="VGD55_05535"
     CDS             1717..1962
                     /locus_tag="VGD55_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869835.1"
                     /translation="MQIDTVGASGGHASLSVGAGVQATATSSDAGRWGPDPRGSAGVT
                     VPGNTTAVADTTMGDTGFAGRSNGIGTSTLGFAVVVL"
BASE COUNT          385 a          676 c          739 g          395 t
ORIGIN      
        1 gacctggaat cccccgctgg tgggatgtcc aaagttggtg ctcccggcca cgctggttcc
       61 gttggtcgaa cgcatgtccc agaagttgcc ggacgtatcc agcacgaaga ggtcgaaccg
      121 aggaggtgcc caggccgaca ggtctggatc acccgtcaga gacgccggat gaccagcgtt
      181 cgtccaggtg aacgaccctc ccgcgaccga gttgccgaca tacatgttcc cgctggtcgc
      241 ccggaatgcc gccacgaccg tccggacgtt ggtcggaaag ccgccggacg tattcgtcgc
      301 gccccaggac gcgacgctga ccttcgacgc cgggctcacg ccgctcggca aattcacggt
      361 ggtccatccc gaccacgaag cgccgtagtt cttggtgttc ttgaggacgc tgctgcccga
      421 gacagcgaac acctggacgt tgccgccacc ccagctgacg gcgtcgggcg actgcgtgaa
      481 gctgttgggg tacgtcagca actcccaggt gaccgtgacc ccttcgtcgt cggaggacgc
      541 gtggtacatc ttcccgttgg tcgcgcgggc aaacgcgtcg acatggccgg atccccagga
      601 cgctacagcc ggcgtgccgg tcaaggtcac gcccgacggc gttccccagt cgatcgtcgc
      661 cgtgaacgcg acggacgtgt tcgcagcgac ggtggtcgtg ccgagatggg cggagctcgt
      721 caggccggcg acgtacgcct tcgtcgaccg tccatagacg cacccgttgc cgacggccga
      781 gaccgcctcg ttgctgaact cgagctgggt ggtgatctgt gagggcgagt tgctggccgg
      841 ctgcgtctgg atcgtgtaat ggatgccgtt gcacaagtcg ccgatctcgc agacggcgcc
      901 ggtgccctga tcccatcctt cgaagttcgc gccgtcgttc cacgcgggat tggcggtggc
      961 ctccgtgatc tcgtgcatga tggcgccctg ttgaaaagca aagccgcacc caccctggcc
     1021 gggataggac acccacgccg tgattgtgtc ttgcgtggaa acgaagtgct ggccacacaa
     1081 gcccgcgacc ggcgggggcg cgttcgacgg cgtgaagatg acgaagatgt cgtcatagct
     1141 ggcggaaacg ttgctggcga cgaaatccgc ctcattcatg atgtccgtgt cactgagcgt
     1201 gatctgcccg gtgaccggat cgggcgccgg cagcccgctc tcgtcgaaga accagaatcc
     1261 atcccgcgtg ggtgcgatct gctcgaacgc actggagttc caatcaaggc ccgcgtactg
     1321 ggtcgtgacg ccatcgaacc gcgtggcgcc gattccaagt gactcgagag gtgcgaccgc
     1381 gttgtagatc gcgagggcgg ttgactcgaa tgattggggg tcgccgtacg tgccatatcc
     1441 ccagaacacg taatagatgt taccgggttg gtcgtatgcg tccacgtagc cgccatagta
     1501 ggacatggtc tggcccgtgt tgcaggcgcg cgccgggtgc gcgttgaaga cagcgccgga
     1561 tgccgccgcg gcgagagtca tgcagaacag tttcgttcca gtggttttca tgatctcacc
     1621 attcctcaag agcagcacgc gggaattgcg tcattggggt acacaccacc ggcgacggtc
     1681 attgcatgac aaccggcggg aagcttgtcc gcggacgtgc agatcgacac agtgggtgcg
     1741 tcgggcggac acgccagctt gagcgtgggc gccggcgtgc aggcgacggc aacatcgagc
     1801 gacgcgggtc gctgggggcc agatccgagg ggcagcgccg gggtgactgt gcccggcaac
     1861 acgaccgccg ttgccgacac aacgatgggc gacaccggat tcgccggccg gtcgaacggg
     1921 atcggcacgt cgacgttggg ctttgcggtt gtcgttcttt gatgaagctt cgtttgtgca
     1981 gggtcaaacg tacgactggt cgagggccgc ggctttgcgg cgacgggtgc gccggtggcg
     2041 gcggctacag ccaccgtttg agggacttcc ggctccgacg cgccttcatc ggtacacccg
     2101 cccaatgcgg gcaacaacgt gaacagaacg gcaagcggcg tgaacgatct catgtgtgac
     2161 ctccctgtcg attggcggtc gcgacgaccg catgt
//
LOCUS       DASWVF010000312         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_441835, whole genome shotgun sequence.
ACCESSION   DASWVF010000312 DASWVF010000000
VERSION     DASWVF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441835"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGD55_05540"
     CDS             complement(<1..257)
                     /locus_tag="VGD55_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869836.1"
                     /translation="MKTAPQRSSVRLGVAIDNAYKQLAAAGVASPRHDAETLAAFVIG
                     CPRGRLLVAGDLSAGQSAVFDKLIARRASRVPLQHLTGSAA"
     gene            complement(266..1336)
                     /gene="prfA"
                     /locus_tag="VGD55_05545"
     CDS             complement(266..1336)
                     /gene="prfA"
                     /locus_tag="VGD55_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0869837.1"
                     /translation="MFEAVEELLLEHADLETKMSDPGVHANPGLARSLGRRYAQLTPI
                     VETYLRWRTLGDDLEAARELGDTDASFRAEMPDLERRRDVAAEELRTLLIPRDPNDGK
                     DVILEVKAGEGGEESALFAGDLLRMYLRYAERQGWKTEVLDANESDLGGYRDATLAVR
                     AGTGDGAWSRLKFEGGVHRVQRVPVTESQGRIHTSAAGVLVLPEAEEVDVEIDPNDLR
                     IDVYRSSGPGGQSVNTTDSAVRITHVPTGTVVSCQNEKSQLQNKESALRILRARLLVL
                     AQEEAAAAASAERRIQIRTVDRSERIRTYNYPENRISDHRVNYKAYNLDQVLDGDLDD
                     LIQALVDADSAARMAAVAEPTS"
     gene            complement(1426..1641)
                     /gene="rpmE"
                     /locus_tag="VGD55_05550"
     CDS             complement(1426..1641)
                     /gene="rpmE"
                     /locus_tag="VGD55_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0869838.1"
                     /translation="MKPDIHPEYTETAVACSCGNTFTTRSTAKGGTIHADVCSACHPF
                     YTGKQKILDIGGRVEKFEKRFGKAAAK"
BASE COUNT          274 a          594 c          610 g          344 t
ORIGIN      
        1 aatgccgcgc tgcccgtgag gtgctggagc ggcacccgcg aagcgcggcg ggcgatcagc
       61 ttgtcgaaga ccgccgactg accagctgac aggtcgcccg caacaaggag gcgtccgcgc
      121 ggacagccga tcacgaatgc cgcgagcgtc tccgcatcat gccgcggcga cgccacgccg
      181 gccgcggcca actgcttgta agcgttgtcg atggcgacgc ccaatcgcac ggaggatcgc
      241 tgcggcgcgg ttttcatgac gtctttcatg acgtgggctc cgcaaccgcg gccatgcggg
      301 ccgcggagtc ggcgtcgacc agggcctgga tcaggtcgtc gagatctccg tctagcacct
      361 ggtcgaggtt gtaggccttg tagttgacgc ggtgatcgga gatccggttc tcggggtagt
      421 tgtaggtgcg gatccgctct gaccggtcga ccgtgcggat ctggatgcgg cgttcggcgc
      481 tagccgcagc agccgcttct tcctgcgcga gtaccaggag gcgggcccgc aagatccgca
      541 gcgcggactc cttgttctgc agctggctct tctcgttctg gcacgacacg acagtgccgg
      601 tcgggacgtg ggtgatgcga acggccgagt cggtcgtgtt cacgctctgg ccccctggcc
      661 cactcgaccg gtagacgtcg atacgcagat cgttcggatc gatctcgacg tcgacctcct
      721 ccgcttctgg caagacgagg acgccggcgg cgctggtgtg gatgcggccc tgcgactcgg
      781 tcacggggac gcgctgcacc cggtggacgc cgccctcgaa cttcagccgg ctccacgcgc
      841 catcgccggt tcccgcgcgc accgcgaggg tcgcgtcgcg gtagccgccg aggtcggact
      901 cgttggcgtc aagcacctcg gtcttccagc cttggcgctc ggcgtagcgc aggtacatcc
      961 gcagcaggtc gcccgcgaac agcgccgact cttcgccgcc ttcacccgcc ttgacctcga
     1021 ggatcacgtc cttgccatcg ttcgggtcgc gcgggatcag cagcgttcgc agttcttctg
     1081 cggctacatc gcgccggcgc tcgaggtctg gcatctcggc gcggaaagaa gcgtcagtgt
     1141 cgccgagttc acgggcggcc tcgaggtcat caccgagtgt tcgccagcgc aggtaggtct
     1201 cgacgatcgg agtgagctgc gcatatcggc gtccaagaga acgcgctaga cctgggttgg
     1261 catgcacccc agggtccgac atcttcgtct cgaggtcggc gtgctcaagg agcagctctt
     1321 cgactgcctc gaacatcacg acctcccctt gcgactagac ccggacatgg tgacggcgcc
     1381 ggttctcagg ggtctaatga gccctgggac cggcgccgtg cgtagctact tggcggctgc
     1441 tttgccgaac cgcttctcga acttctcgac gcgaccgccg atgtcgagga tcttctgctt
     1501 gcccgtatag aacgggtggc aggcggagca gacgtcggcg tggatcgtgc cgcccttcgc
     1561 ggtactgcgc gtggtgaacg tgttcccaca gctacaggcc acggctgttt cggtgtactc
     1621 gggatggatg tcgggcttca ctggttctcc taagactgtc gcggccgccg ggtcgtccct
     1681 gcgttggcag gcttactgac gtgaaccgga accgacttgg accgaccttc agtgtgacag
     1741 accggcttga cactcgccta acgcccgtcc gggctgcccg attcccgctg aatcggacgg
     1801 cgagtggcta gtcgttgggc ta
//
LOCUS       DASWVF010000313         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_365059, whole genome shotgun sequence.
ACCESSION   DASWVF010000313 DASWVF010000000
VERSION     DASWVF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365059"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGD55_05555"
     CDS             <1..261
                     /locus_tag="VGD55_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY0869839.1"
                     /translation="ALAVSDPSDNDSEESDPDDPDPGLLSDDDAEGPDVLRVRISGAV
                     ARAFTKRALAIVAAGRPPCQFCGQPLDPESHVCARSNGHHPH"
     gene            272..1003
                     /locus_tag="VGD55_05560"
     CDS             272..1003
                     /locus_tag="VGD55_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEY0869840.1"
                     /translation="MTEQSFELSAADVRAVLTDGDLTIEGRLVDASNTTLYAEVTHGA
                     VSLTCVYKPIRGERPLWDFPDGTLAGREVGAYLVSEATGWDVVPPTVMRDGPLGPGAC
                     QLWINIDEDADVLALLHSDHPGLRRLAVFDAVVNNADRKGGHLLPTPGGHIYGVDHGV
                     CFSTEDKLRTVLWQWRGTALPDDALDTLAALRIDLAGDLGVQLKQLITPREVSATRRR
                     IDRLLREGCFPMPGDGWPAVPWPPF"
     gene            1027..2280
                     /gene="mshC"
                     /locus_tag="VGD55_05565"
     CDS             1027..2280
                     /gene="mshC"
                     /locus_tag="VGD55_05565"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEY0869841.1"
                     /translation="MHPWQAPSTLPALPGRGELPRVWDTSAADAVVVGSSGAASMYVC
                     GITPYDATHLGHAATYIGFDLLNRVWRDAGVDVSYVQNVTDVDDPLLERASATGETWQ
                     HLATREIELFHADMAALRVLPPVHYVGVVESIPDVAGLIQTMLDAGVAYDVEGDLYFS
                     VASASDFGAVSHDDHATMLALFAERGGDPERLGKKDPLDCLLWRAERPGEPAWDTPIG
                     RGRPGWHVECTAIALKYLPAPFDVQGGGRDLVFPHHEMCAAQASALGHHPFARSYVHG
                     GMVGYAGAKMSKSLGNLVFVSTLRDSGVDPMAIRLALLAHHYQSDWEYTAADLELANA
                     RLRSWRHATALSSGPAAHGLLADVRERLADDLDAPGAIAAIDDWAAYAAVDAEAAAAT
                     DGVRIDPDAPGLVRAIADALLGIAL"
     gene            2293..2754
                     /locus_tag="VGD55_05570"
     CDS             2293..2754
                     /locus_tag="VGD55_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0869842.1"
                     /translation="MFRVAFVEPRIPPNTGNAIRMVAATGCELHLIGPLGFDLSDAKL
                     RRAGLDYHDLATVKVHLDLAAAWASLMPARVFAFTARGSTTFTDIAYQPGDVLMFGPE
                     PDGLSDDVLADPRVTSWVRIPMLSGRRSLNLSNAAAIAVYEAWRQHKFAGS"
     gene            complement(2967..>3577)
                     /locus_tag="VGD55_05575"
     CDS             complement(2967..>3577)
                     /locus_tag="VGD55_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY0869843.1"
                     /translation="LAAPAAGRRDIILVRGLEPNMRWRSFCGEIIDVAVGLGVEEVVN
                     LGALLSDSPHSRPVPVTGTAVDGDTARRLGLEQSRYEGPTGIVGVFNDACIQAGLPAV
                     SLWAAVPHYVAQPPCPKATLALLRNLEDVLETPIPYGDLAEMSEAWERGVDQLAEEDS
                     EVAEYVRTLERQKDEAELPEASGEAIAREFERYLRHRRDGSA"
BASE COUNT          550 a         1209 c         1200 g          618 t
ORIGIN      
        1 gctctcgcgg tcagcgaccc ctccgacaac gattcagaag agtccgatcc cgacgacccc
       61 gacccaggct tgctgagtga cgacgacgcc gaaggccccg acgtactacg ggtgcggatc
      121 agtggcgcag tcgcacgcgc gttcaccaag cgagcgttgg cgatcgtcgc tgccggtcgg
      181 ccaccgtgcc agttctgcgg gcaaccactc gacccggaaa gccacgtttg tgcacggtcc
      241 aacggccatc acccgcactg acgggtgtgt cgtgaccgag cagagtttcg aactcagtgc
      301 cgccgatgtg cgcgccgtgc tcaccgatgg tgacctcacc atcgagggcc ggctcgtcga
      361 tgcgtcgaac accacgttgt acgccgaggt cacgcacggc gcggtctcgc tgacgtgtgt
      421 ctacaagcct attcgcggcg agcgcccact gtgggatttc cccgacggca ccctcgccgg
      481 tcgcgaggtc ggtgcgtact tggtgtccga ggccaccggc tgggacgtcg tgccacccac
      541 cgttatgcgc gacggaccgc tcggtcctgg cgcgtgccag ctttggatca atatcgacga
      601 ggacgccgac gtgctggcgt tgctgcacag tgaccacccg gggttgcgca ggcttgcggt
      661 gtttgacgcg gtcgtgaaca acgccgaccg caagggcggt cacttgctgc cgacgccagg
      721 cggccacatc tacggcgtcg accatggcgt gtgtttctcg accgaagaca agctgcgtac
      781 ggtgttgtgg cagtggcgcg gcaccgcgct tcccgacgac gctctcgaca cccttgccgc
      841 gctgcggatc gacctggccg gcgacctcgg cgtccaattg aaacaactca tcaccccgcg
      901 cgaagtgtcc gcgacccggc gtcgcatcga ccggctgttg cgcgaagggt gtttcccgat
      961 gcccggcgac ggctggcccg ccgtcccgtg gccgccgttc tagcggcggc tggctagagt
     1021 tcgagcatgc acccctggca ggccccatcg acgcttcccg cactgcccgg gcgaggtgag
     1081 ttgccacgcg tgtgggacac ctcggccgcc gatgcggtcg ttgtcggctc gtccggcgct
     1141 gcctcgatgt acgtgtgcgg catcaccccg tacgacgcca cccacctcgg gcacgccgcg
     1201 acctacatcg ggttcgacct gctcaacagg gtgtggcgcg atgcaggcgt cgatgtcagc
     1261 tatgtgcaga acgtgaccga cgtcgacgat ccgctgctcg aacgggcatc cgcgacgggg
     1321 gagacctggc agcacctcgc gactcgcgag atcgagctct ttcacgccga catggcggcg
     1381 ctgcgcgtat tgccgccagt gcactacgtg ggggtggtcg agtcgatccc tgacgtggct
     1441 gggttgatcc agacgatgct cgatgcgggc gttgcttacg acgtcgaggg cgacctctac
     1501 ttctctgtcg catcggcatc cgacttcggt gcggtgtcgc acgacgatca cgcgacgatg
     1561 ctcgcgttgt tcgccgagcg cggcggcgac cccgaacgac tgggcaagaa ggacccgctc
     1621 gactgcctgc tgtggcgcgc cgagcgacca ggagagccgg cgtgggatac cccgatcggg
     1681 cgtggtcggc cgggatggca cgtcgagtgc accgcgatcg cactgaagta cctacctgca
     1741 cccttcgacg tgcagggtgg cggtcgtgat ctggtcttcc cgcaccacga gatgtgcgca
     1801 gcccaggcga gcgcgctcgg ccatcatccg ttcgcgcgca gctatgtgca cggcggcatg
     1861 gtcggctacg caggcgcaaa aatgtcgaag tcgcttggga acctggtgtt cgtgtcgacg
     1921 ctgcgcgaca gcggtgtcga cccgatggcg atccggctgg cactgcttgc gcaccactat
     1981 caaagcgact gggagtacac cgctgccgat ctcgagttgg cgaatgcccg gctgcggtcg
     2041 tggcgtcatg cgactgcgtt gtcgagcggg ccggcagctc atgggctgct cgcggacgtg
     2101 cgcgagcgtc ttgctgacga cctcgacgcg ccgggcgcga ttgccgcaat cgatgactgg
     2161 gcggcatacg cagccgtcga tgcggaagct gcggcggcaa ccgatggtgt ccgtatcgac
     2221 cctgacgcac cggggctggt ccgggcgatc gccgacgcgt tgctcggcat tgctctctag
     2281 ggctctgtag gcgtgttccg cgtcgcgttc gtcgagccgc gcattccgcc caacaccggc
     2341 aacgcgatcc gcatggtcgc agcgaccgga tgcgagctgc acctgatcgg acccctcggg
     2401 ttcgacctct ccgatgccaa actgcgtcga gcgggcctcg actaccacga tctcgcgacg
     2461 gtgaaggtgc atcttgacct tgctgcggcg tgggcgtcgc tcatgcccgc cagggtgttc
     2521 gcgttcactg cgcgcggcag caccacgttc accgacatcg cgtaccagcc cggcgacgtc
     2581 ctgatgttcg gccctgagcc cgacggcctg tccgatgacg tgctcgccga tccgcgggtg
     2641 accagctggg tccggattcc gatgctgtcc ggacgccggt cgctgaacct gtcgaatgcg
     2701 gccgcgatcg cggtctacga agcctggcgc cagcacaaat tcgccggaag ttgaccccgc
     2761 ccccggcccc cggaagttga tcacgccctc ggaagttgat cacgtttacg tgatccacgt
     2821 acccgaggac aggctcacac gactgtcttc gggtacgcca gcctgtcctc ggccggacca
     2881 agatcatcag ccgggacggc ggggtggctt gtgtcgcagc ggtggtgcag gtgggcggtg
     2941 gtgcaggtgg gctcgtggtg cgggcgtcag gcggagccgt cgcggcgatg ccgcaggtag
     3001 cgctcgaact cgcgagcgat tgcctcacca ctcgcctcgg gcagctcagc ttcgtccttt
     3061 tgccgttcga gggtgcgcac gtactcggcg acctcggaat cctcttcggc caactggtcg
     3121 actccgcgct cccatgcctc tgacatctcg gccagatcgc cgtaaggaat cggcgtctcg
     3181 agcacgtcct cgaggttgcg cagcagcgcc agggtcgcct tggggcaggg cggctgcgcc
     3241 acgtagtggg ggactgccgc ccacagcgag accgccggca gtccggcctg gatgcaggcg
     3301 tcgttaaaca cgcccacgat cccggtcgga ccttcgtaac gcgactgctc gagcccgagc
     3361 cggcgcgctg tgtcgccgtc gaccgccgta ccggtcacgg gaacgggccg gctgtgcggg
     3421 gagtcggaca gcagcgcacc gaggttgaca acctcttcga ccccgaggcc gaccgcaacg
     3481 tcgatgattt cgccgcaaaa cgaccgccag cgcatgttcg gctcgaggcc gcgcaccaag
     3541 atgatgtcgc gacgacccgc tgctggcgcg gccagcg
//
LOCUS       DASWVF010000314         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_263536, whole genome shotgun sequence.
ACCESSION   DASWVF010000314 DASWVF010000000
VERSION     DASWVF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263536"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VGD55_05580"
     CDS             complement(<1..631)
                     /locus_tag="VGD55_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY0869844.1"
                     /translation="MTATAVAPIALVGSGEYLPVMTEIEGALIAGRPPRYVQLATAAA
                     PEGEGSLRRWHALGKQQADRLGVQQVVVDVRTRDDADQPAFADAIAGAGLIYLSGGNP
                     AYLAETLRDTAVWRAIVAAHSAGAAVAGCSAGAMALADWAPRMRDLTSSAPSGLGLVS
                     HVRVIPHFDTMPSRVPDVLRNALLRLPEGTTLLGVDEDTALVGGPHDWTV"
     gene            complement(628..1791)
                     /locus_tag="VGD55_05585"
     CDS             complement(628..1791)
                     /locus_tag="VGD55_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869845.1"
                     /translation="MIVGMTRVLMILGSGETSPAMVTPHQQLFARLETNARAVLLDSP
                     YGFQENADELTQRTIQYFADSVGRKVEAVTFRSTPVSDPVAHALEMAKLRGAGWIFAG
                     PGSPSYALHTWAGSAVPDALHAVLRDGGAVVFASAAALTLGLVTVPVYEIYKVGSVPQ
                     WLDGLDIFGRATGLRAAIVPHWNNAEGGTHDTRFCYLGERRLRMLEDTLPDGAFVLGI
                     DEHTGLVIDLDTGAAEVVGRGSVTVRSNVREWPVATGASTTLAELAEHADVEPPSGAV
                     GLGVSAGPVAALDQALEQRDVRSALALLAEVDRGSSTSEERQAVGSYLARCIAALGDS
                     VDVTPYIELLLRQRARAREDKRWSDSDAIRDGLSALGIEVRDSPDGASWGFTR"
     gene            complement(1694..2650)
                     /locus_tag="VGD55_05590"
     CDS             complement(1694..2650)
                     /locus_tag="VGD55_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869846.1"
                     /translation="MTRRGLQITLGVLWLLDAGLQFQPYMFTTKFAVEVIDPAGAGQV
                     PFVSTPIEHVVNVISAHPAPYNAVFASVQLAIGLGLLLRRTVVPALVASIVWSLSVWY
                     LGEGLGGLTGHNPALLAGAPGAALLYAILAVAAWPNNHKLPASWLVFAWAGYWVGGAI
                     LQVWRGPRIGPDLAATVSESANGAPGWLSRIDFSLARTLGHVSPGFVAGWIGVQVLIG
                     LAAFAPRRARRIAVLAGCLIAVGFWIIGPGFDQLISGHGTDPNTPPLVVIIGVAIFAY
                     HRRSHVLGMSDDRRHDASADDSRFGRDQPRHGDAAPATVRAT"
     gene            complement(2668..>3276)
                     /locus_tag="VGD55_05595"
     CDS             complement(2668..>3276)
                     /locus_tag="VGD55_05595"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0869847.1"
                     /translation="TTPVPCAGVNHADFAANGRYLIVSCEFDGRVLKVDTVTKQVVGV
                     MQLTPDAMPQDVKLSPDGRVFYIADMQSNGVWEIDGDTFKKIGFLPTGKGAHGLYVSR
                     DASVMYVTNRGEGSISLIDLATRTVKTTWKIPGGGSPDMGGLSADGTQLWVSGRYSGV
                     VYEISTIDGHLLKKIKVGSGPHGLCVYPQPGRYSLGHTGVFR"
BASE COUNT          608 a         1113 c         1056 g          499 t
ORIGIN      
        1 gcaccgtcca gtcgtgcgga ccgccgacga gtgcggtgtc ttcgtcgacg ccgagcaagg
       61 tggtgccctc tggcaaacgc aacagcgcgt tgcgtagcac gtcgggaact cgtgaaggca
      121 tcgtgtcgaa gtgcgggatc acccgaacgt gtgacaccag cccgagcccg ctcggtgcag
      181 agctggtgag atcgcgcatg cgcggggccc agtcggccaa cgccatcgca cccgcgctgc
      241 aaccggcgac cgcagcgccc gctgagtgcg ccgccacgat cgcacgccac acggcagtgt
      301 cgcgcaaagt ctccgcgaga tacgccggat tgccgcccga caaatagatc aaccccgcac
      361 ccgcgatggc atcagcgaac gccggctgat cggcgtcgtc gcgagtgcgc acgtcgacga
      421 ccacttgctg cactccgagc cgatccgctt gctgcttgcc gagcgcgtgc caccggcgca
      481 acgagccttc gccctcgggt gccgcggctg tcgcgagttg cacgtaccgc ggcggccgtc
      541 cggcgatgag cgcgccctcg atttcggtca tcaccggcag gtactcacct gaccccacga
      601 gcgcgatcgg cgcgacggcg gtggcagtca tcgggtgaac ccccagctcg cgccgtccgg
      661 actatctctg acctcgatgc cgagcgcgct cagaccgtcg cgaatcgcat cggagtcact
      721 ccaccgcttg tcttcgcggg cgcgcgctcg ctgcctgaga agcaactcga tgtacggcgt
      781 gacgtcgact gaatccccga gcgccgcgat gcaacgcgca aggtacgacc cgacggcttg
      841 gcgctcttcg gatgtggacg aacctcggtc aacctccgcc aacaaggcaa gagcagagcg
      901 gacgtcgcgt tgctcgagag cctgatcaag tgccgcgacc gggccggccg aaacaccgag
      961 cccgacagca ccagacggag gctcgacgtc tgcatgctcg gcaagctcgg ccaacgtggt
     1021 gctcgcgccg gtggcgaccg gccactcgcg gacgttcgat cgcaccgtga ccgagcctcg
     1081 gccaacgacc tcggcagcgc cggtgtcgag atcgatcacg agacccgtgt gctcgtcgat
     1141 ccccaggacg aacgccccgt ctggcaacgt gtcttcgagc atgcgcagcc ggcgctcgcc
     1201 gaggtagcag aagcgagtgt cgtgcgtgcc gccctcggcg ttgttccagt gcggcacgat
     1261 cgccgcccgc aagccggtgg cgcgaccgaa gatgtcaagg ccgtcgagcc actgcggcac
     1321 cgagcccacc ttgtagatct catagaccgg tacggtgacg agccccagcg tcaatgcggc
     1381 ggccgacgcg aacaccaccg cgccgccgtc gcgcaacacc gcgtgcaacg cgtcgggcac
     1441 cgcgctaccc gcccaggtgt gcagtgcata cgacgggctg cccgggccgg cgaaaatcca
     1501 gcccgcacct cgcagcttgg ccatctccag ggcgtgcgcg acggggtcgg acaccggagt
     1561 cgatcggaac gtgacagcct cgaccttgcg gccgacgctg tcagcgaagt actgaatcgt
     1621 gcgttgcgtc agctcgtcgg cgttctcctg aaacccgtac ggtgagtcga gcagcaccgc
     1681 ccgcgcgttc gtctcaagtc gcgcgaacag ttgctggtgc ggcgtcacca tggcggggct
     1741 ggtctcgccc gaaccgagaa tcatcagcac tcgcgtcatg ccgacgatca tcgctcatcc
     1801 ctaagacatg gctgcgtcgg tggtaagcaa aaatcgcgac gccgatgatg acgacgagcg
     1861 gcggcgtgtt ggggtcggtg ccgtgtccgc tgatcagctg gtcgaaccct ggcccgatga
     1921 tccagaaccc gacggcgatc agacagcctg cgagcacggc aatccggcgc gcccgtcggg
     1981 gggcaaacgc ggcaagcccg atcagcactt ggacgccgat ccaaccggcg acgaagccgg
     2041 ggctcacatg tccaagcgtt cgggccagcg agaagtcgat gcggctgagc caacccgggg
     2101 caccgttggc cgactcggag accgtcgctg ccaggtcggg tccgatgcgg gggccgcgcc
     2161 agacttggag aatcgcacca cccacccagt agccggccca ggcgaacacc aaccacgacg
     2221 ccgggagctt gtgattgttt ggccacgccg ccacggccaa gattgcgtaa agcaacgccg
     2281 cgccgggtgc gccggcgagc agtgcgggat tgtgtccggt cagaccgccg aggccctcgc
     2341 ctagatacca gaccgagagg gaccaaacga tcgaagcaac aagcgcgggg acgacggttc
     2401 ggcgcagcag gaggcccaag ccgatcgcca attgaactga ggcaaacacc gcgttgtacg
     2461 gggctgggtg tgcactgatg acgttgacga catgctcgat cggcgtgctg acgaacggta
     2521 cctggccggc accggcgggg tcgatcacct cgaccgcgaa cttcgtcgtg aacatgtagg
     2581 gctggaattg caagccggca tcgagcagcc agagcacgcc gagcgtgatc tggagacctc
     2641 gccgcgtcat ctgcaaccaa ctccgagtta gcggaaaacc ccggtgtgac ccaaggagta
     2701 ccggccgggt tggggatata cgcacagccc gtgcgggccg ctcccgactt tgatcttctt
     2761 gagcaggtgg ccatcgatcg tgctgatctc gtacacaacg ccgctgtagc gacctgacac
     2821 ccacagttgc gtaccgtcgg ccgacaagcc acccatgtca ggcgagccac cgccggggat
     2881 cttccacgtc gtcttcaccg tgcgggtcgc gaggtcgatc agcgagatag atccctctcc
     2941 gcggttggtc acatacatga cggaagcgtc acggctcacg tagagcccat gcgcgccctt
     3001 gccggtcggc aagaaaccga tcttcttgaa cgtgtcgccg tcgatctccc acacgccgtt
     3061 gctctgcatg tcggcgatgt agaagactct gccgtcaggc gagagcttca cgtcctgcgg
     3121 catcgcgtca ggagtcagtt gcatgacacc gacgacctgc ttggtcaccg tgtcgacctt
     3181 caacacacgg ccgtcgaact cgcagctaac aatgaggtag cggccgttgg ccgcgaagtc
     3241 ggcgtggttg acgcctgcac atggcaccgg tgtcgt
//
LOCUS       DASWVF010000315         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_161194, whole genome shotgun sequence.
ACCESSION   DASWVF010000315 DASWVF010000000
VERSION     DASWVF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161194"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VGD55_05600"
     CDS             complement(<1..1517)
                     /locus_tag="VGD55_05600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEY0869848.1"
                     /translation="MTIATPPAPGANAGAAKPPAPAGPPPPVRPGLVTLTVDGFEISV
                     PKGTLVIRAAEFLGIQVPRFCEHPLLDPVGACRQCLVEVEGQKKPMASCTTTVTEGMV
                     VKTQITSAVAEKAQNGVIELLLVNHPLDCPVCDKGGECPLQNQTMTNGYSESRFRDVK
                     RTYPKPIAISAQVLLDRERCVLCTRCTRFAQQIAGDPFIEMPERSALEQVAVYEDQPF
                     NSYFSGNTVQICPVGALTGTQYRFRARPFDLVSSPSICEHCASGCQQRNDHRRGKMLR
                     RLAGVDPQVNEEWNCDKGRWAFSYATSPDRLDTPLVRDEDGELVPTSWPEALRAAAAG
                     LAAARNEAGVGVLAGGRLTVEDAYAYAKFARVALGTNDIDFRARVHSDEELAFLGARV
                     AGRFLDVTYDDLEAASTVLLVGLEPEEESPIIFLRLRKAAFKKHAKIVSIAPFATRGL
                     DKMRGRLIATVPGGEATMLQVLASGETVEVPPGVDATGWSELLTEVAAALALPSSV"
     gene            complement(1517..>1722)
                     /locus_tag="VGD55_05605"
     CDS             complement(1517..>1722)
                     /locus_tag="VGD55_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY0869849.1"
                     /translation="GDEQDIETLLDLSENILGRAFCALGDGATSPVTSSIQYFRDEYI
                     AHFDGKGCPFDPVASTVFAGASA"
BASE COUNT          292 a          570 c          580 g          280 t
ORIGIN      
        1 acgctcgacg gaagcgcgag ggccgccgcc acttcggtaa gcagctccga ccaaccggtc
       61 gcgtcaacac caggcggcac ctcgacggtc tcacccgagg cgagcacttg cagcatcgtc
      121 gcttcgccgc ccggcactgt cgcgatcagg cggccacgca ttttgtcgag accacgcgtc
      181 gcgaacggcg cgatcgacac gatcttggcg tgcttcttga atgcggcctt gcgcagccgc
      241 aggaaaatga tcggcgactc ttcctcgggc tcgagcccga cgagcagcac ggtcgatgcc
      301 gcttcgaggt cgtcgtacgt cacgtcgagg aagcgaccgg caacgcgcgc gcctaagaac
      361 gcgagctctt cgtcggagtg cacccgcgcc cggaagtcga tgtcgttggt gcccaaagca
      421 acgcgcgcga acttcgcgta ggcataggcg tcttcgaccg tcaggcgccc gccggcgagc
      481 acgccgacgc ccgcctcgtt acgtgcggct gcgagaccgg cggcggcagc acgcagcgcc
      541 tcgggccacg atgtcggaac gagctcgccg tcctcgtcac gaaccagcgg cgtgtcgagc
      601 cggtcgggcg aggtggcgta actgaaggcc caccggccct tgtcacaatt ccactcctcg
      661 ttcacctgtg ggtcgacgcc ggccaagcgg cgcagcatct ttccgcgacg gtggtcgttg
      721 cgctgctggc agcccgacgc acagtgctcg cagatgcttg gcgacgacac caggtcgaac
      781 ggacgagccc ggaaccggta ctgcgtgccg gtcagtgcgc cgaccgggca gatctgcaca
      841 gtgtttccgg agaagtaaga gttgaacggc tggtcctcgt agaccgcgac ctgttcgagt
      901 gccgagcgct cgggcatctc gatgaacggg tcaccggcaa tctgctgcgc gaaccgggtg
      961 cacctcgtgc agagcacgca ccgttcgcgg tcgagcagta cttgcgccga aatggcgatc
     1021 ggcttggggt acgtgcgctt cacatcgcgg aagcgcgact cggagtagcc gttggtcatg
     1081 gtctggtttt gcagcgggca ctcgccgccc ttgtcgcaga ccgggcagtc gagcgggtgg
     1141 ttgacgagca gcagttcgat gacgccgttc tgcgccttct cggcgaccgc cgacgtgatc
     1201 tgcgtcttga cgaccatgcc ctcggtcacc gtcgtcgtgc acgaggccat cggctttttc
     1261 tgaccttcga cctcgacgag gcactggcgg cacgcaccga ccgggtcgag cagggggtgc
     1321 tcgcaaaaac gcggcacctg aatgccgagg aactccgcgg cacgaatcac caaagtgccc
     1381 ttcggcaccg agatctcgaa cccgtcgacc gtcaacgtga cgagccccgg acgcaccggc
     1441 ggcggcggtc cggccggtgc gggtggcttc gcagcgccag cgttcgcgcc gggcgcggga
     1501 ggggtggcga ttgtcatcag gcactcgctc cggcgaaaac ggtcgacgcg acggggtcga
     1561 acgggcaacc cttgccgtcg aagtgggcga tgtactcgtc gcggaagtac tggatcgacg
     1621 acgtcaccgg gctggtcgcg ccgtcaccga gcgcgcaaaa cgcgcggccg agaatgttct
     1681 cggagaggtc aagcagcgtt tcgatgtctt gctcgtcgcc ct
//
LOCUS       DASWVF010000316         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_33951, whole genome shotgun sequence.
ACCESSION   DASWVF010000316 DASWVF010000000
VERSION     DASWVF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33951"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGD55_05610"
     CDS             <1..226
                     /locus_tag="VGD55_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869850.1"
                     /translation="TTSTTPPALLAAQGFKMSNKTVDVTVYTYASGTTTGGSTSLSQP
                     GGYCVKVTSGSTKPFYYVSFLGGITNTACP"
     gene            284..943
                     /gene="nucS"
                     /locus_tag="VGD55_05615"
     CDS             284..943
                     /gene="nucS"
                     /locus_tag="VGD55_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY0869851.1"
                     /translation="MRLVIARCSVDYVGRLTAHLPSAQRLILVKADGSVSIHADDRAY
                     KPLNWMSPPCTLQEAPGVWTVTNKAGEQLVLTIEELLHDSSHELGVDPGLVKDGVEAH
                     LQVLLAAHPDALGEGMALVRREYPTPIGPVDLLCRDGAGVSVAVEIKRRGEIDGVEQL
                     TRYLELMNRDALIAPVRGIFAAQEIKPQARVLAVDRGIECITIDYDALRGADDPTARL
                     F"
     gene            1049..1378
                     /locus_tag="VGD55_05620"
     CDS             1049..1378
                     /locus_tag="VGD55_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0869852.1"
                     /translation="MDTTQLLKGVLDLAVLAVLSERDGYGYDVVRRLRESGLDDVGDA
                     SVYGTLRRLFQAGALTSYVVPSEEGPHRKYYGLNDHGRSVMALSAKTWIGFAQTMDHL
                     LSKGAPA"
     gene            1375..>2365
                     /locus_tag="VGD55_05625"
     CDS             1375..>2365
                     /locus_tag="VGD55_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869853.1"
                     /translation="MTATTASIEVRDYLAAVSRELADLPADERDDLLEDLDSHLHEVI
                     AEGEGSLEQRLGPPAQYAAELRASAGLSSSDRSTANALHRVVSSLSASSIWRRTAEHP
                     WTRATLAFLPQLRPAWWIVRAWLAVGIIAGVYRQRGFNDASITDIYHDSGLWPGGYGH
                     RYIGIALLVIAMPISIQLGRRSLRGSARWLVVAGNVIAVALLWPALSGLNNQTYIVDS
                     GQSVPTDGLYNNGNQVTNIYPYDAQGQPLDHVRLFDQDGEPLLRVNDVPAIAVDGVPV
                     ATPAPSAYANEYPQPNTTTIYGPDGQPTTAVLPRPNIVVPPLPSASATPPSPSP"
BASE COUNT          432 a          811 c          727 g          395 t
ORIGIN      
        1 gaccacttcg acgacgccgc cggcgctact ggccgcgcag ggcttcaaga tgtccaacaa
       61 gaccgtcgac gtcacggtct acacgtatgc cagtggtacg acgaccggcg gttcgacgag
      121 cctgtcgcaa ccgggagggt actgcgtaaa agtaacctcg gggtccacca agcccttcta
      181 ctacgtgagc ttcctcggcg ggatcaccaa caccgcttgc ccgtaacgcg atccgcccgc
      241 cccacgtcgt ccggtgaatg ctctgcccgc taacgttcgg cgtgtgcggc tggtcattgc
      301 tcggtgcagc gtcgactatg tcggccggct gacagcccat ttgccgagcg cgcagcggct
      361 gatcctcgtc aaagcggacg gttccgtctc catccacgcc gacgaccggg cgtacaaacc
      421 gctgaactgg atgagcccgc cgtgcacgtt gcaagaagcc cccggcgtct ggaccgtgac
      481 gaacaaggcc ggcgagcaac tcgtgctcac catcgaggag ctgttgcacg actcgagtca
      541 cgaactcggc gtcgatcctg gcctggttaa agacggggtc gaggcgcacc tgcaggtgct
      601 actagcggca caccccgacg ctctcggcga aggcatggca ctggtgcgtc gcgaatatcc
      661 gacaccgatc ggcccagtcg acttgctgtg ccgcgacggc gccggcgtga gcgtcgcggt
      721 cgagatcaag cgccgcggtg agatcgacgg cgtcgagcaa ctcacgcgct acctcgaact
      781 gatgaaccgt gacgccctca tcgcgccggt gcgtggcatc ttcgcggctc aggagatcaa
      841 gccgcaggcg cgggtgctcg ctgtcgaccg cggcatcgag tgcatcacga ttgactacga
      901 cgcgctgcgc ggtgccgacg acccgaccgc ccgcctcttc taaccgccgg tcgccccagc
      961 ccggcgtgtc ctcttgtgcg gtactgcgca ttacggagta gggtgcatcg cgtaggagct
     1021 acagcactcg caccctcgga ggtcggttat ggacaccacc caactgctca agggggtgct
     1081 cgacctcgca gtgctcgccg ttctgtccga acgcgacggc tacggctacg acgtcgtgcg
     1141 ccggttgcgc gaatccgggc ttgacgacgt aggcgacgca tccgtgtacg gcacgctacg
     1201 gcggctgttt caggccggcg cgctgacgtc gtacgtggtg ccgagcgaag agggtccgca
     1261 ccgcaagtac tacggattga atgaccacgg ccgctcagtc atggcgttgt cggccaagac
     1321 ctggatcgga ttcgctcaga ccatggacca cctgctcagc aaaggagcgc cagcatgacc
     1381 gcgacaactg cgtcaatcga ggttcgcgac tacctcgctg cggtgagtcg cgaacttgct
     1441 gacttgccgg cagacgaacg cgacgacctg ctcgaagacc tcgacagcca tttgcatgaa
     1501 gtgatcgccg agggcgaggg ctcgctggaa cagcgactcg gtccgcccgc gcaatatgcc
     1561 gccgaactgc gagcgtccgc cggtctctcc tcctccgacc gctccacagc aaatgcgttg
     1621 catcgtgtgg tgagctcgtt gagcgcgagc tcaatctggc ggcgcaccgc agaacaccca
     1681 tggacgcgcg cgaccctcgc gttccttccg caactacgcc ccgcatggtg gatcgtgcgg
     1741 gcgtggctgg cggttggcat catcgccggc gtctaccgcc agcgcggctt caatgacgcg
     1801 agcatcaccg acatctacca cgactcaggc ttgtggcccg gcggctacgg gcaccggtac
     1861 atcggcatcg ctttgctcgt gattgcgatg ccaatctcga tacaacttgg acgtcgatca
     1921 ctacgcggga gcgcccgctg gctcgtcgtc gcgggcaacg tcatcgcggt ggcgctgttg
     1981 tggcccgcac tgtcgggcct caacaatcag acgtacatcg tcgacagcgg ccaatccgtt
     2041 ccaaccgacg ggctctacaa caacggcaac caagtgacga acatctaccc ctatgacgcg
     2101 caagggcagc cgctcgacca cgtgcgactc ttcgaccagg acggcgagcc attactccgc
     2161 gtgaatgacg tgcccgctat tgccgtcgac ggggtcccgg tggcgacgcc tgcaccgagc
     2221 gcctacgcaa acgagtaccc gcagccgaac acgacgacga tctacggccc agacggacag
     2281 ccgaccacgg ccgtgctacc gcgcccgaac atcgtcgtac cgccgctgcc gagtgcgtct
     2341 gcgacgccac catctccgtc gccga
//
LOCUS       DASWVF010000317         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_84788, whole genome shotgun sequence.
ACCESSION   DASWVF010000317 DASWVF010000000
VERSION     DASWVF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84788"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VGD55_05630"
     CDS             complement(<1..859)
                     /locus_tag="VGD55_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY0869854.1"
                     /translation="MTFGLTPVLTANWGTPESWTLAEYRRTGGYDSLRTALAMEPDAL
                     IAMVKDSGLRGRGGAGFPTGMKWGFIPQGDGKPHYLVVNADESEPGTCKDIPLMMASP
                     HTLLEGVLIAAYAIRANQAFIYVRGEVLHVVRRLLNAVEEAKAAGLIGTNVLGFGYDI
                     DVVVHIGAGAYICGEETALLDSLEGFRGQPRLRPPFPAIEGLYACPTVINNVETIASV
                     PAIVRGGAAWFKKMGTEKSPGFKIFSLSGHVAKPGQYEAPLGITLRELLDATGGMREG
                     HELKFWTPGG"
     gene            complement(856..1701)
                     /gene="nuoE"
                     /locus_tag="VGD55_05635"
     CDS             complement(856..1701)
                     /gene="nuoE"
                     /locus_tag="VGD55_05635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0869855.1"
                     /translation="MRPLSHSHGEPNGLPTVTLDPRLSPGATLADPLSDDAKARVREL
                     GAQVIARYPRARSAMMPLLYLVQAEQGYVSPDGIKLVAELLGLTTAEVAAVSTFYTMY
                     KRRTCGDYHVGVCTNTLCAILGGDAIFAALKEHLGVGNAGTTPDNVVTLEHIECNAAC
                     DYAPVVMVNWEFFDNQTVESAKELVDKLRSGELPLPTRGAPLVTHIESSRVLAGFPDG
                     LVGEGPSAGAESMVGIELAKERGETAPAFDKQPAAAPPKTAAPAKGAAAPAKIAAPPK
                     GQDAS"
     gene            complement(1689..1817)
                     /locus_tag="VGD55_05640"
     CDS             complement(1689..1817)
                     /locus_tag="VGD55_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869856.1"
                     /translation="MHLREPSFVNLQAVPAVCEGSLIADVIASVASLDPVMGGCDR"
BASE COUNT          319 a          605 c          594 g          320 t
ORIGIN      
        1 agccccccgg cgtccagaat tttagttcgt ggccctcacg cattccgcca gtagcgtcga
       61 gcagttcgcg caacgtgatg ccaagcggcg cctcgtattg acctggcttc gcgacgtggc
      121 cggagagaga gaaaatcttg aatccgggcg acttctcggt gcccatcttc ttgaaccagg
      181 cggcgccccc gcgcacgatc gccgggacgc tcgcgatggt ctcgacgttg ttgatgacgg
      241 tcggacacgc atagagacct tcgatcgccg ggaacggcgg ccgcagtcgc ggctgaccac
      301 ggaatccttc gagcgagtcg agcagtgcgg tctcttcacc acagatgtag gcaccggcac
      361 cgatgtgcac gacgacgtcg atgtcgtatc cgaacccgag aacgttggtg ccgatcaagc
      421 cggctgcctt cgcctcttcg accgcattga gcaaccggcg aacgacgtgc agcacctcgc
      481 cgcgaacgta gatgaacgcc tggttcgccc gaatcgcata cgccgcgatg agcacgccct
      541 caagcagggt gtgcgggctg gccatcatca acggaatgtc tttgcaggtg ccgggttcgc
      601 tctcgtcggc gttgacgacg aggtagtgcg gcttgccgtc gccttgcggg atgaatcccc
      661 acttcatgcc ggtcgggaac ccggcgccgc cacgaccccg tagtccggaa tccttgacca
      721 tcgcaatgag agcgtccggc tccatcgcca gtgccgtacg cagcgagtcg taaccgccgg
      781 tgcgtcgata ctccgcaaga gtccacgact ccggcgtgcc ccagttcgcc gtcaacaccg
      841 gcgtgagccc gaaggtcatg aggcgtcctg gcccttgggt ggggcagcga tcttggctgg
      901 ggcggcggcg ccctttgctg gggcagcggt ctttggtggg gcagcggccg gctgcttgtc
      961 gaacgcgggc gcggtctcgc cgcgctcctt ggcgagctcg ataccaacca tggactccgc
     1021 acccgcggag ggcccttcgc cgaccaatcc gtcgggaaag cctgcgagca cgcgcgacga
     1081 ctcgatgtgc gtcaccaacg gcgcgccacg tgtcggcaag ggcagttcac ctgagcgcaa
     1141 cttgtcgaca agctccttcg cgctctcgac ggtctggttg tcgaagaact cccagttcac
     1201 catcacgacc ggggcataat cgcacgccgc gttgcactcg atgtgctcga gggtcaccac
     1261 gttgtcgggc gtcgtgccgg cgttgccgac cccgagatgc tctttaagcg cagcgaaaat
     1321 agcgtcgcca ccaaggatcg cgcacagcgt gttcgtgcac acgccgacgt gatagtcgcc
     1381 gcaggtgcgc cgcttgtaca tcgtgtagaa cgtgctgacc gcggcgactt cggcggtcgt
     1441 cagcccgagc agttcggcaa ccagcttgat gccatcgggc gagacgtagc cctgctcggc
     1501 ctgcacgagg tagagcaacg gcatcatggc cgaccgggcg cggggatacc gcgcaatgac
     1561 ctgggcaccg agttcgcgga cccgcgcctt tgcgtcgtcc gataaaggat cggcaagcgt
     1621 cgcgcccggg ctgagtcgcg ggtcaagcgt gacggtcggc aagccgttcg gctcgccatg
     1681 gctgtgactc aacggtcgca cccgcccatc accgggtcga ggctggcaac ggaggcgatg
     1741 acgtcggcaa tgaggctgcc ctcacacacg gctggcactg cctggaggtt cacaaacgat
     1801 ggctcgcgca ggtgcactcg atacggcttc gtaccgcc
//
LOCUS       DASWVF010000318         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_59201, whole genome shotgun sequence.
ACCESSION   DASWVF010000318 DASWVF010000000
VERSION     DASWVF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59201"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGD55_05645"
     CDS             <1..294
                     /locus_tag="VGD55_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869857.1"
                     /translation="FWQDDKPNPAVFSRDEATNDAWPQVLAWFNQRLTGDGWARDDTA
                     AARGNDNVSLAWAWTKADETFTLTVYSEASRDALYKQAPELRGRSIALETFLS"
     gene            complement(362..2848)
                     /gene="pheT"
                     /locus_tag="VGD55_05650"
     CDS             complement(362..2848)
                     /gene="pheT"
                     /locus_tag="VGD55_05650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0869858.1"
                     /translation="MRVGLSWLREVVAIPATESGHDVAERFVRAGFEVESVEVEGSVT
                     GDLAVGEIVSFTDEPQTNGKTIRWCQVRIADGAEPRGIVCGAHNFAVGDRVVVALPGA
                     VLPGGFEISARKTYGHISDGMICSERELGIGDDHTGILVLAPDAPVGADAIAVLGLGD
                     EVLDVSPTPDRGYAMSVRGLARELAAAYRVPFVDPAQRVGVAIATADGGWPVRIDDPS
                     GCDRFVAQVVRGIDPSRSSPEWMRRRLQTAGVRSVSLAVDLTNYVMLELGQPLHAYDL
                     AKLQGPIVVRRATPAERVRTLDDVDRALHPDDLLIADDSGAIGIAGVMGGASTEIDST
                     TSQVLIEGAHFTSSVISRSARRHGLLSEASRRFERGVDSAVAPVAVQRAADLLAEFGG
                     GTVETTATDVGQPAPLPTIRMSTTLPADVVGVDYDLDEVLGSLLAVGCQVAQSGNELA
                     VGVPSWRPDLRDPFDLVEEVARLVGYDRIPSVLPVPPPSGGLTEAQRARRRIAIGLAD
                     AGYVEAPSYPFIGVADLDALGLAADDPRRRTLALANPLSDEQPALRTTLLPGLLATLR
                     RNISRGNTDVALFETGLVYRIDGRENVLVRPPRLPVDRRPTDDELAALNAALPRQPRR
                     LAVALAGERERPGWWGQGRAVAWGDAVEAARIVAREAGVVLDVAADEHPPWHPGRCAV
                     LRVEGAVVGHAGELHPRVIEAFGLPPRTAAAELDLDAVVAAAQGLAVAPTLSTYPLAT
                     SDVALVVAADVPAAEVENALRDGAGPLLEALRLFDVYVGPGVPEGKRSLAYRLSLRAP
                     DHTLTADEANAIRDAAVAEVGRRVGAVLRS"
     gene            complement(2850..3959)
                     /gene="pheS"
                     /locus_tag="VGD55_05655"
     CDS             complement(2850..3959)
                     /gene="pheS"
                     /locus_tag="VGD55_05655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0869859.1"
                     /translation="MSAPNSNYDPVEVSPLHADEVERLRHEALAAIGAATDLDELRQL
                     RVAHAGDRSPLALANREIGALLPHAKADAGRRVGQARAAVAAALADREAALLVQRDSR
                     ILVEEAVDVTLPWDRTPRGARHPISTVTDRIVDVFVSMGWGVAEGPEVEAEWFNFDAL
                     NTPADHPARALQDTFFVESADSGVVLRTHTSPVQARTLLSRKPPIYIVAPGRTFRTDE
                     LDATHTPVFNQCEGLAVDEGLTMANLRGTLDAFAEAMFGVGVVTRLRPHFFPFTEPSA
                     EVDLRCWVCHGESVGHPENPCRTCRSEGWIEWGGCGMVNPRVLIACGVDPQRYTGFAF
                     GMGIERTLLSRHGGGDMRDVVEGDVRFALAFGPES"
     gene            complement(4002..>4517)
                     /locus_tag="VGD55_05660"
     CDS             complement(4002..>4517)
                     /locus_tag="VGD55_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0869860.1"
                     /translation="GELLDVSRIDAGRLELRKQVVDIAAAVRRLIDGRIASGDSPDRF
                     EIEVAEGLPEMWLDPDKLDQILANLVDNGIRHGAGTVTIAISPAGGEPPSGLTLTVSD
                     HGEGVASEIRNLVFRRFWRAGRRGGTGLGLYIVKGLVEAHGGEVAAADAPGGGASFRV
                     TLPTGTPAFAL"
BASE COUNT          779 a         1577 c         1505 g          656 t
ORIGIN      
        1 ttttggcagg atgacaagcc gaacccggcg gtcttctcgc gcgatgaggc gacgaacgac
       61 gcctggccgc aggtgctggc ctggttcaat cagcggctca ccggcgacgg ttgggcgcgc
      121 gatgacaccg ccgcggccag aggcaacgac aacgtctcgc tcgcgtgggc atggaccaaa
      181 gccgacgaga cattcacgct gaccgtgtac tccgaagcaa gccgggacgc cttgtacaaa
      241 caggcaccag aactgcgggg ccgttcgatc gccctcgaga cgttcttgag ctagcgcgcg
      301 gcgagtctga actgctgtcc gcgcacgcca accactccac cggctaaccg acgcgaaccg
      361 ctcagctgcg caggaccgcg cccactcggc gtccgacctc ggcgaccgct gcatcgcgga
      421 tcgcattggc ctcgtcggcc gtgagcgtgt ggtcaggcgc ccgtaacgac aagcgatagg
      481 ccagcgagcg cttgccctcg ggcacgccag gacccacgta gacgtcgaac aggcgcaacg
      541 cctcgagcag cggacctgct ccgtcgcgta gcgcgttctc gacctcggcg gccgggacgt
      601 cggcggcaac gaccagcgcg acgtcggacg tcgcgagcgg atacgtcgac aaggtcggtg
      661 cgaccgcgag gccttgagca gcagccacca ccgcgtcgag gtcgagctcc gcggctgcag
      721 tacgcggggg caatccgaac gcttcgatca cccttgggtg cagctctccc gcgtggccga
      781 cgacggcgcc ctcaactcgc aggactgcgc aacgccccgg atgccacggc gggtgttcgt
      841 cagcggcgac gtcgagcaca acgcccgcct cgcgcgcgac gatccgcgcc gcttcgaccg
      901 cgtcgcccca ggccaccgcc cgcccttgac cccaccaacc cggtcgttcg cgctcgcccg
      961 ccaacgccac cgcaagtcgc cgcggctggc gcggtaacgc tgcgttcagc gccgcgagct
     1021 cgtcgtcagt cggccggcga tcgacgggga gccgcggcgg tcgcaccaac acgttctcgc
     1081 gaccgtcgat gcgatacacc agcccggttt cgaacaacgc gacgtcggtg tttccgcgag
     1141 agatgttgcg gcgcaacgtc gcgagcagac ccggtaacag cgtcgtacgc aacgccggct
     1201 gctcgtcgga gagcgggttc gccaatgcga gcgtgcgacg ccgcgggtcg tcggctgcca
     1261 ggccgagcgc atcgagatct gccacgccga tgaacgggta gctcggcgcc tcgacatacc
     1321 cggcatcggc cagcccgatc gcgatgcggc gccgagcccg ttgcgcttcg gtgagtccac
     1381 cgctcggtgg cgggaccggc agcacggaag ggattcggtc gtagcccacc agccgggcga
     1441 cctcttcgac cagatcgaac gggtcacgca aatcagggcg ccaactcggg acgccgacgg
     1501 ccaactcatt gccggactgc gcgacctggc acccgaccgc gagcaacgac ccgagcacct
     1561 cgtcgaggtc gtaatcgacg ccgacaacat cggccggcag cgtcgtgctc atgcggatcg
     1621 tgggcagcgg cgccggctga ccgacgtcgg tcgctgttgt ttccacggtg ccgccgccga
     1681 actcggcgag caggtctgcg gcgcgctgca cagcgacggg cgccaccgcg gaatcgacac
     1741 cgcgttcgaa ccggcgcgat gcttcgctga gcaagccgtg tcgccgcgcg cttcgcgaaa
     1801 tgacggacga cgtgaaatgg gcgccttcaa tgagcacctg cgatgtcgtc gaatcgatct
     1861 cggtggaagc gccgcccatg acgccggcga tgccgatcgc gccgctgtcg tcagcaatca
     1921 gcagatcgtc tgggtgcagc gcgcgatcga cgtcgtcgag cgtgcgcact cgctcggcgg
     1981 gcgtcgcacg gcggaccacg atggggcctt gcagtttcgc gaggtcgtag gcgtgcagcg
     2041 gctgtccgag ttcgagcatc acgtagttcg tgaggtcgac ggcaagactc acggaccgca
     2101 cgccggcggt ctgaaggcgg cgacgcatcc attcaggtga gctccggctg gggtcgatcc
     2161 ccctgaccac ctgcgcgacg aagcgatcgc agccacttgg gtcgtcaatg cgcaccggcc
     2221 agccgccgtc ggcagttgcg atggcaaccc cgacgcgttg cgcggggtcg acgaacggga
     2281 cgcgataggc ggctgcaagt tcgcgggcga gaccgcgcac cgacatcgcg tagccgcggt
     2341 cgggcgtcgg cgacacgtcg aggacctcat cgccgaggcc gagcaccgca atcgcgtcgg
     2401 cgccgaccgg ggcgtcgggt gcgagaacaa ggatgcccgt gtgatcgtcg ccgataccga
     2461 gctcgcgctc ggagcagatc atgccgtcac tgatgtgacc gtaggtcttg cgtgcggata
     2521 tctcgaagcc gccagggagc acggcaccgg gcagcgcaac gaccacccga tcgccgaccg
     2581 cgaagttgtg cgcgccacac acgattccgc gcggctcggc gccatcggcg atgcgcacct
     2641 ggcaccaccg aatcgtcttg ccgttggttt gcggctcgtc agtgaacgac acgatctcgc
     2701 ccactgcaag gtcaccggtg acggaccctt cgacctcgac ggactcgacc tcgaatccgg
     2761 cccggacaaa tcgctcggcg acatcgtggc cactctcggt ggccgggatt gcgacgactt
     2821 cacgcaacca cgaaagacct acgcgcacgt cacgactccg gaccgaacgc cagcgcgaag
     2881 cgcacgtcac cctcgacgac atcacgcatg tcgccaccgc catgacgaga cagcagcgtg
     2941 cgctcgatgc ccatgccgaa ggcgaatccg gtgtaccgct gcggatcgac gccacacgcg
     3001 atcagcaccc gcggattgac catgccgcac ccaccccact cgatccagcc ctcggaacgg
     3061 caggtgcgac acggattctc tgggtggcca acagactcgc catgacacac ccagcagcga
     3121 aggtcgactt cggcagacgg ctcggtgaac gggaagaagt gcggccgcag cctcgtcacg
     3181 acgccaaccc cgaacatcgc ctcggcgaac gcgtcgaggg tgccgcgaag gttagccatc
     3241 gtgagccctt cgtcgaccgc aagaccctcg cactggttga acaccggcgt gtgcgtggca
     3301 tcgagctcgt cggtgcgaaa cgtgcggccg ggcgccacga tgtagatggg tggcttgcgt
     3361 gacagcaaag tgcgcgcctg caccggcgag gtgtgcgtgc gcaacacgac tccactgtct
     3421 gccgactcga cgaagaacgt gtcttgaagc gcgcgcgccg ggtgatcggc cggcgtgttc
     3481 agcgcgtcaa agttgaacca ctcggcttcg acctcaggcc cctcggctac gccccaaccc
     3541 atcgacacga acacgtcgac gatgcggtcg gtgaccgtcg agatcgggtg tcgtgctccg
     3601 cgcggagtgc gatcccacgg cagcgtgaca tctacggcct cttcgaccag gatccgggag
     3661 tcacgctgga ccaggagtgc tgcttcacgg tcggccaatg ccgcggcaac tgcagccctg
     3721 gcctgaccca cgcggcgtcc ggcatcagcc tttgcatggg gcagcaacgc gccgatctcg
     3781 cggtttgcga gcgcgagtgg tgaccgatcc cccgcgtgcg cgacccgcag ctgccggagc
     3841 tcgtcgaggt cggtggcagc gccgatggca gcgagcgctt catggcgcag gcgctcgacc
     3901 tcgtcggcgt gcagtggact cacctcgacc gggtcgtagt tgctgttcgg ggcggacatg
     3961 gcaggtgact ctccagagcg ggcggggatc agttgctgag tctagagcgc gaaggccggc
     4021 gtcccggtcg gaagcgtcac ccgaaacgac gcgccaccac ctggcgcgtc ggcggctgcc
     4081 acctcgccgc cgtgcgcttc gacgaggcct ttcacgatgt agaggccgag tccggtgccg
     4141 ccacgacggc cggctcgcca gaatcgccgg aacaccaggt tgcggatctc cgaagcgaca
     4201 ccctcaccgt ggtcagagac ggtcaaggtg agtccgcttg gtggctcgcc gccggccggc
     4261 gatatcgcga tcgtcaccgt gccggcgccg tgccggatcc cgttgtcgac gaggttggcc
     4321 aggatctggt cgagcttgtc cgggtcgagc cacatctcgg gcagcccctc ggcgacctcg
     4381 atctcgaacc ggtcgggtga atcccccgac gcgatgcgcc cgtcaatgag ccgtcgcacc
     4441 gcggccgcga tgtcgaccac ttgcttgcgc agctcgaggc gcccggcgtc gatgcgcgag
     4501 acatcgagca gttcacc
//
LOCUS       DASWVF010000319         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_314155, whole genome shotgun sequence.
ACCESSION   DASWVF010000319 DASWVF010000000
VERSION     DASWVF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314155"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGD55_05665"
     CDS             complement(<1..351)
                     /locus_tag="VGD55_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0869861.1"
                     /translation="MVATAQPTGGGMPSRDELAVWRAFLRAHAQLIRTLESELGAARE
                     IPLASYDVLVQLAEAPAHRLRMTELADRVLLSRSGLTRLIDRLERDGLVRREAVPDDA
                     RGMYAVLTAAGTDRL"
     gene            385..1527
                     /locus_tag="VGD55_05670"
     CDS             385..1527
                     /locus_tag="VGD55_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0869862.1"
                     /translation="MVETARPTDEQVRAALATVQDPDIRRPITELGMVESVVIDSAGH
                     VTVGILLTVPGCPLKEKITSDVTAAVRTVAGVAGVEVNLGYMTDEQRKALQVQLRGPG
                     KPEKEIPFNNPNSLTRIIAIASGKGGVGKSSVTINVAAALAARGLSVGLLDADVYGHS
                     VPRMLGIAARPTQVAGMIMPPQAHGVRVISIGMFVEGNTPVAWRGTMLHRALNQFLTD
                     VYWGDLDVLLVDLPPGTGDIAISLAQFVPGAELIVVTTPQLAAAEVAERAGTIALQTR
                     QRVAGVIENMAWLPCPHCGERVDVFGSGGGQVVVDALSRAFGTEIPLLGQIPLDPGLV
                     SAGDTGMPFVLSHPNAPASLELIRIAESLAIRERSLVGRPLGLQPA"
     gene            complement(1540..1767)
                     /locus_tag="VGD55_05675"
     CDS             complement(1540..1767)
                     /locus_tag="VGD55_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869863.1"
                     /translation="MAAAGFAASVVVGLGGAISGSVAVTGTAGSVGAAGPGAPTTTLP
                     AAALSVVAEPAVAVVPRPGGRVAVSVVYRRP"
BASE COUNT          301 a          647 c          639 g          290 t
ORIGIN      
        1 caaacggtct gttcctgcag ccgtgagcac tgcgtacatg ccgcgggcgt cgtcgggtac
       61 cgcctcgcgt cgcacgaggc cgtcacgctc gagccggtcg atcagtcggg tcaagccgct
      121 gcgtgacagc agcacgcggt cagcaagctc cgtcatgcgc aggcgatgcg ccggcgcttc
      181 ggccaactgc acgagcacgt cgtaggacgc caacggaatc tctcgtgccg caccgagctc
      241 ggattcgagg gtgcggatca gctgtgcgtg ggcacgcagg aacgctcgcc agactgcaag
      301 ttcgtcgcgc gagggcatgc cgcccccggt tggctgcgct gttgcgacca cgtgacgcat
      361 gctagggccg cgtaggatcg gggagtggta gaaacagcgc ggccgaccga cgaacaggta
      421 cgagccgcac tagcaacggt tcaagaccca gacattcgcc gtccgatcac cgagctcggc
      481 atggtcgaat cggtcgtcat cgacagcgct ggtcatgtca cggtcggcat tctgctcacc
      541 gtgccgggtt gcccgctcaa ggaaaagatc acctccgacg tcacggcggc ggtccgcaca
      601 gtggcgggcg tcgctggggt cgaggtcaac ctgggctaca tgaccgacga gcagcgcaag
      661 gcgctgcagg tgcagctgcg tggccccggc aagcccgaga aggaaatccc gttcaacaac
      721 cccaattccc tgacgcgcat catcgcgatc gcatcgggca agggcggcgt cgggaagtct
      781 tccgtcacga tcaacgtggc ggccgccctt gcggcgcgcg ggctctcggt gggcttgctc
      841 gatgccgacg tctacggcca ttcggtgccg cggatgctcg gcatcgcggc tcggcccacg
      901 caagtggccg gcatgatcat gccgccgcaa gcacacggcg tccgggtcat ctcgatcggc
      961 atgttcgtcg agggcaacac tccggtggcg tggcgcggca ccatgctgca ccgggcgctg
     1021 aatcagttcc tgaccgacgt ctactggggt gacctcgacg tcttgctcgt cgacctgcca
     1081 cctggtaccg gtgacatcgc catctcgctc gcgcaattcg tccccggcgc cgagttgatc
     1141 gtcgtcacga cgccgcaact tgccgccgcc gaggtggccg agcgggccgg cacgatcgcc
     1201 ctgcaaacgc ggcaacgcgt agctggtgtc atcgagaaca tggcgtggtt gccgtgcccg
     1261 cactgcggcg agcgagtcga cgtgttcgga tcgggcggcg gccaggtggt ggtcgatgcg
     1321 ctctcgcgcg cgttcggcac cgagattccg ctgctcggcc agatcccgct cgaccctggc
     1381 ctcgtcagcg ccggagacac cggcatgccg ttcgttctca gccacccgaa cgcgccggct
     1441 tcgctcgaac tgatccgcat cgcggagtca ctcgcaatcc gcgagcggag cctcgtcggg
     1501 cgccccctgg gcctgcaacc ggcctgacgt tcagccctgt cagggcctgc ggtaaacgac
     1561 cgacacggcg actcgaccgc ccggccgcgg aacgacggcc acggcaggtt cagcgacaac
     1621 gctcagggcg gcggccggca aggtcgtggt gggcgctcca ggaccggccg cgcccacgct
     1681 gccggcagta cccgtcaccg cgacgctgcc cgagatcgca ccgccaaggc cgacgacgac
     1741 cgatgccgcg aagcctgcgg ctgccaccgt tcgattacgc ggacggcggt ttggtgcagg
     1801 tacgcctgtc ttcaacggtc gcgacggcgc gctgcggccc aggagccggc gcgatcgtga
     1861 gcgtcgggga gtgcgcg
//
LOCUS       DASWVF010000320         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_212616, whole genome shotgun sequence.
ACCESSION   DASWVF010000320 DASWVF010000000
VERSION     DASWVF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212616"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VGD55_05680"
     CDS             <1..632
                     /locus_tag="VGD55_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869864.1"
                     /translation="AYGAVLAAMRGDAAVASLALPVVSQLRASEDQQDQAIVYLLRAF
                     TEAASGQLSDVMRDAQELVDRIESLGIGAETVRWAWPLAVRTAFELADAEAVTRLISV
                     VDAFPRGHLPPILRAERDLASARLRVGSGDRAAGELVREAIDELRAVGSPYHLAWGLL
                     DLAEYSIDVGAAEVASDAIAEAREIATRLRVQPILDRAHQLAAGAAIAA"
     gene            complement(599..1789)
                     /locus_tag="VGD55_05685"
     CDS             complement(599..1789)
                     /locus_tag="VGD55_05685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0869865.1"
                     /translation="MDAETFAALMTDDGRSAVDTAEGLLDAGLSALAVGEQVRRDFTA
                     LPPHVASAAVGQATLRRRGRVKFGADADQMWFTPDGLEQATSAPVARYRSSRFAALAG
                     DLGRPPRVADLCCGIGGDLRALAAAGCAVTGFDRDPVTVMAARANGAEDVECVDVETL
                     DLAGFDAVFIDPARRLSGRRTFDVAAYLPPWSFVIAALRAAPAAAAKLAPGIPHEIVP
                     EGFEAEWVSMGGGLKEAVLWAGSFTDGMRRRATLLPEGASMSSSSDADELAPSGPLRH
                     YLYEPDDAVVRAHLIGDLARSVDGVLLDPTTAYITSDHFVATPFAQAFEVHDVMPFSL
                     KRLRTALRDRHVGSVTIMKRGSAIDVEQLRRNLRLSGTESAVVVLALIGGRHHAAIAA
                     PAAS"
     gene            2008..2313
                     /gene="groES"
                     /locus_tag="VGD55_05690"
     CDS             2008..2313
                     /gene="groES"
                     /locus_tag="VGD55_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0869866.1"
                     /translation="MTTATKVAIRPLEDRIVVQAVEAETTTASGLVIPDTAKEKPQEG
                     IVLAIGPGRFEDGQRVPLDVKEGDRVLYSKYGGTEVKYGGEEYLVLSARDVLAVIDK"
     gene            2432..4069
                     /gene="groL"
                     /locus_tag="VGD55_05695"
     CDS             2432..4069
                     /gene="groL"
                     /locus_tag="VGD55_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643281.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0869867.1"
                     /translation="MPKILQYDESARRSLERGVDALANAVKVTLGPKGRNVVISKSYG
                     VPTITNDGVTIAREIELEDRHENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVHEG
                     LRNVAAGANPLALKRGIEAAVTAVNERLLDTAREVSGKEEIAHVAAISAQDPEVGALI
                     ADAFDKVGKDGVITVEESNTMGLELELTEGLQFDKGYISMYFVTDNERMETVLENPLI
                     LINHGKISSVSDLLPLLEKVIQASKPLLIIAEDVEGEALSTLVVNKIRGTFVSAAVKA
                     PAFGDRRKAMLEDIAILTGGQVVAPEVGLKLDDVGLEVLGTARRVTITKDDTTIVDGA
                     GSAEAVADRVRQLRAEIERTDSEWDRDKLQERVAKLAGGVAVIRVGAATEVELKEKKH
                     RLEDAISATRAAVEEGIVAGGGSALVHAASALDKLDLVGDEATGVSIVRKALVEPRRW
                     IAENAGLEGYVVTEKVAALDLGHGLNAATGEYTDLIADGVIDPVKVTRSALANAASIA
                     ALLLTTETLVVEKPEEPEGDAHGSHGGHSHGPGGHSH"
     gene            4134..>4849
                     /locus_tag="VGD55_05700"
     CDS             4134..>4849
                     /locus_tag="VGD55_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869868.1"
                     /translation="MVRDKAAAPDALAALLTKGERAAFHGPPGTAVPPLEEAVALATG
                     SGRPAEATAATWLLGVAQASRGRFGTALTLLGGVMGPLRAALAGEDTAAELRLFGALA
                     AATVASVHRQLGRHTVAWEFDALGLTAADAMPGLDEAVFDCRLGLASDAVGLGEIEIA
                     GTELAVAAGVAEVHPDWWRQRVRLDWVRAEIALLGDDPTAAQEAAAEAIDRAELARAP
                     RHVAKGLLFLGVSQAHLGSS"
BASE COUNT          834 a         1611 c         1651 g          753 t
ORIGIN      
        1 gggcgtacgg agccgttctc gcggcaatgc ggggcgacgc agccgtcgca tctttggctc
       61 taccggtcgt ttcccagctg cgcgcgagcg aggatcaaca agaccaagcg atcgtctacc
      121 tcctgcgggc gttcaccgag gcggcgagcg gccagttgag cgacgtcatg cgcgatgcgc
      181 aggagctcgt cgatcggatc gaatccctcg gcattggtgc cgagactgtg cggtgggcgt
      241 ggccactcgc tgtccgcact gccttcgagc tcgccgacgc cgaggccgtc acgcggttga
      301 tatccgtcgt cgatgctttc cctcgggggc atttgccgcc gatcctgcgc gccgagcgtg
      361 acctggcgag tgcacgcctt cgagttggta gcggggatcg agcggcgggc gagctagtgc
      421 gcgaagcgat cgatgaactg cgtgcggtcg gcagtccgta tcacctcgcg tggggattgc
      481 tcgatctcgc cgagtactca atcgatgtcg gtgcggccga ggttgcctct gacgcgatcg
      541 ctgaggcgcg cgagatcgcc actcgacttc gggttcaacc gattctcgac cgcgcacatc
      601 aactcgctgc gggcgctgcg atcgcggcgt gatgacggcc accaataaga gcgaggacga
      661 cgacagcgct ctcggtacct gacagtcgca ggttgcgtcg cagctgctcg acatcaattg
      721 cggagcctct cttcatgatg gtcacgctgc cgacgtggcg gtcgcgcaac gcggttcgca
      781 gccgcttcaa cgagaacggc atgacgtcgt gcacctcgaa ggcctgagcg aacggcgtcg
      841 cgacgaaatg gtcgctggtg atgtaggccg tggtcgggtc gagcaggacg ccgtcgaccg
      901 accgcgccag gtcgccgatc aggtgtgcgc gcacgaccgc gtcgtcgggt tcatagaggt
      961 agtgcctgag cggtcccgag ggcgccagct cgtcggcgtc cgatgacgaa ctcatgcttg
     1021 cgccctcggg caggagagtg gcgcggcggc gcatgccgtc ggtgaagctg cctgcccaga
     1081 gcacggcctc cttcaggccg ccgcccatcg acacccactc ggcctcgaag ccttcgggga
     1141 cgatctcgtg ggggatgcca ggcgcgagct tcgcggcggc cgccggcgcc gcgcgcagtg
     1201 ccgcgatcac gaacgaccag ggcggcaagt acgccgcgac gtcgaacgtg cggcgtcccg
     1261 agagacgtcg ggccggatcg atgaagaccg cgtcgaagcc ggccaggtca agggtttcga
     1321 cgtcaacgca ctcgacgtct tctgcaccgt tcgctcgggc ggccatgacc gtcactgggt
     1381 cgcggtcgaa gccggtcact gcgcacccag ccgccgcgag tgcgcgcagg tcgccgccga
     1441 tgccgcagca aagatcagcc acccgaggcg ggcggcccag gtcaccggcg agagcggcga
     1501 agcggcttga gcggtaccgg gcaacgggtg cactcgtcgc ttgctcgagg ccgtcagggg
     1561 tgaaccacat ctggtcggcg tccgccccga acttcacccg accgcgacgt cgaagggttg
     1621 cctggccgac cgcggccgac gcgacgtgcg gcggcaaagc ggtaaagtcg cggcgcacct
     1681 gctcaccgac cgcgagggcg gacagaccag cgtcgagcaa tccctcggcg gtgtcgactg
     1741 ccgaccggcc gtcgtcggtc atgagggccg cgaacgtctc ggcgtccatc gcacgtcccg
     1801 tctggcgcgc gctgtcacgg gccaacaaca gccaacaaca gccaacaaca gcctggcact
     1861 cgggttgacg gagtgctaag cccagcatag tcttcggcat ggagtagctg gcactctcgt
     1921 gctgtgtttg ctaacccggc ctgacccccg cgacggcagg ccaaaccgac gtccacattc
     1981 cactgatcaa ggagggggag tcacccagtg acgaccgcca ccaaagtagc catccgccca
     2041 cttgaagacc gcatcgtggt tcaggccgtc gaggccgaga ccacgaccgc atccggtctc
     2101 gtcatccctg acaccgccaa ggagaagcct caagagggca tcgtcctggc gatcggccca
     2161 ggtcgtttcg aagacggcca gcgggtgccg ctcgacgtca aggaaggcga ccgcgtgctg
     2221 tacagcaagt acggcggcac cgaggtcaag tacggcggcg aggagtacct cgtgctctcc
     2281 gcccgtgacg tcctcgcggt catcgacaag tagtccgcta gcccatgcac gtctgacccg
     2341 tcgccccgga gccgcaaggc accggggcgg cgtgcgtcat cccagctttg aggtttgacc
     2401 gttcgcgcaa tcccgagagg acccgtagcg catgccgaag atcctgcagt acgacgagag
     2461 cgcccgccgt tcgcttgaac gtggcgtcga cgcactcgcc aacgccgtca aggtgaccct
     2521 cggcccgaag ggccgcaacg tcgtcatctc caagagttac ggcgtcccga ccatcacgaa
     2581 cgacggcgtc acgatcgctc gcgagatcga gctcgaagac cgccatgaga acctcggtgc
     2641 ccagctcgcc aaggaagtcg cgacgaagac caacgatgtc gcaggcgacg gcaccacgac
     2701 tgcgaccgtg cttgcgcagg ccatggttca cgagggtctg cgcaacgtcg ccgccggcgc
     2761 caacccgctc gccctgaagc gaggcatcga ggccgctgtc accgctgtga acgagcggct
     2821 gctcgacacg gcgcgcgagg tgtccggcaa ggaagagatc gcccatgtcg ccgcgatctc
     2881 agcccaagat cccgaggtcg gtgcgctgat cgccgatgcg ttcgacaagg tcggcaagga
     2941 cggcgtcatc acggtcgaag agtccaacac gatgggcctc gagcttgagt tgaccgaggg
     3001 tcttcagttc gacaagggct acatctcgat gtacttcgtc accgacaacg agcgcatgga
     3061 gacggtgctc gagaacccgc tcatcctcat caaccacggc aagatttcgt cggtctccga
     3121 tctgttgccg ctgcttgaga aggtcatcca ggcaagcaag ccgctgctga tcatcgccga
     3181 ggatgtcgaa ggcgaagcgc tctcgacgct ggttgtgaac aagatccgcg gcaccttcgt
     3241 atcggccgca gtgaaggctc cggccttcgg tgaccgccgc aaggccatgc tcgaagacat
     3301 cgcgatcctc acgggtggcc aggtcgttgc cccagaggta ggtctgaagt tggacgacgt
     3361 ggggctcgag gtcctcggca ctgcacgccg cgtgacgatc acgaaggacg acacgaccat
     3421 cgtcgacggc gccggctcgg ctgaggcggt cgctgacagg gttcgtcagt tgcgcgccga
     3481 gatagagcgc accgactccg agtgggatcg cgacaagctc caggagcggg tcgccaagct
     3541 tgctgggggc gtcgcggtga ttcgcgtcgg cgcagccacc gaggtcgagc tcaaggaaaa
     3601 gaagcaccgc ctcgaagacg cgatctcggc cacccgggca gcggtcgaag agggcatcgt
     3661 ggccggtggc ggttctgcgc tggtccatgc ggcatcggct ctcgacaagc tcgatctcgt
     3721 cggtgacgag gcgacgggcg tctcgatcgt ccgcaaggca ctcgtcgagc cgcggcggtg
     3781 gatcgccgaa aacgccggtc tcgagggata cgtcgtgacc gagaaggtcg cagcacttga
     3841 cctcgggcac gggctcaacg ccgcgaccgg tgagtacacc gacctgatcg ccgacggcgt
     3901 catcgacccc gtcaaggtga cccgttcggc actcgcgaat gcggcgtcga tcgcagcctt
     3961 gctcctcacc accgagactc ttgtggtcga gaagccggaa gagcccgagg gcgacgctca
     4021 cgggagccac ggcggccaca gtcacggccc gggcgggcac agccactaac cctcaagtgc
     4081 tgccacccgc gattcgcccg gttttgggcg gtcggctcca gtagcctccg cgtgtggtcc
     4141 gtgacaaagc agctgcgccg gacgcgcttg ccgccttgct caccaagggc gaacgggcgg
     4201 cctttcacgg gccgcccggc accgccgtcc cgcccctcga agaggccgtc gcgttggcga
     4261 cgggcagcgg tcggcctgct gaagcgacgg cagcgacctg gttgctcggg gtcgcgcagg
     4321 cttcacgggg gcgctttggc accgcgctga ctctgctcgg tggggtgatg ggtccgttgc
     4381 gcgcggccct ggccggtgaa gacaccgccg ctgaactgcg gcttttcgga gctctcgccg
     4441 cggctacggt ggccagcgtg caccgccagc tcggcaggca cacggtcgca tgggaattcg
     4501 acgcgctcgg cctgaccgcg gctgatgcga tgccagggct cgacgaggca gtcttcgact
     4561 gccggctcgg tcttgcgtct gacgccgtgg gactcggcga gatcgagatc gccggcaccg
     4621 aactcgcggt ggcggcggga gtggctgaag tgcaccccga ctggtggcgc cagcgggtcc
     4681 ggctcgactg ggtgcgggct gagatcgcat tgctcggcga cgatccaacg gcagcgcagg
     4741 aggcggccgc cgaggcgatc gaccgcgccg aacttgcgcg cgccccgcgc cacgtcgcca
     4801 agggcttgct gtttctcggc gtctcccaag cgcacctcgg cagctccga
//
LOCUS       DASWVF010000321         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_339858, whole genome shotgun sequence.
ACCESSION   DASWVF010000321 DASWVF010000000
VERSION     DASWVF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339858"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGD55_05705"
     CDS             <1..525
                     /locus_tag="VGD55_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0869869.1"
                     /translation="DVASVGAGLGRVQTVSRWRMQVSERSDGLIVINDAYNANPESTR
                     AALRALAAMGAGDRQRRTHAVLGVMAELGAGSVGAHRELGETAAHLGVHRLVVVGAGA
                     QGIADGALDAGLDPTRVVVVDDVAAATALLESDLGPDDVVLVKASRSADLQRVAEALL
                     AAPAPVAAPNGGGR"
     gene            522..1583
                     /gene="mraY"
                     /locus_tag="VGD55_05710"
     CDS             522..1583
                     /gene="mraY"
                     /locus_tag="VGD55_05710"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0869870.1"
                     /translation="MKGILAAGTIALVISLFGTPFAIRLLTSRGYGQLIRDDGPTTHH
                     TKRGTPTMGGTVILLAAILGYAAGNMLSASGPTASGLLVLGLATGLGLVGFLDDFIKV
                     RMQRNLGLRARTKLLGQTLVAVVFGILVLQFPDSSGIHPASTHISFVRDTTVALGAVG
                     FVIWALVLVSSTSNGVNLTDGLDGLATGASTMVFAAFVIMTFWQGRNDCLTKAAAGCY
                     PVRDPFDLAILAAAFAGACAGFLWWNAAPARIFMGDTGSLALGGALAGMAIMTRTELL
                     LAVLGGLFVVITMSSIIQIGYFKMTHRRVFKMAPLQHHFELKGWAEVTIVIRFWIIAG
                     LAVALGLGVFYAEWITWQK"
     gene            1571..>2753
                     /gene="murD"
                     /locus_tag="VGD55_05715"
     CDS             1571..>2753
                     /gene="murD"
                     /locus_tag="VGD55_05715"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0869871.1"
                     /translation="MAEVTVSWLPSAAYDAPWHELTVCVAGVGVSGAACARVLRSLGA
                     AVIAVDAGVGDRQQQTAAELAHIGVDVRLGDGDTLPEAVQLVVTSPGWRRDSPLFIAA
                     ASSGTPIWGEPELAWRLREPDAAPWLVVTGTDGKTTTTLMLESMLHAAGHRAASAGNI
                     GAALVELVNGPYDALAVELGSFQLHWSPSVAPLAAAVLNVAPDHLDWWADSFEDYAAA
                     KGLAFAHADTCAVGNADDAVSTRLLARAPGRRVAFTLNSPRPHQLGVVEDLLVDRAFV
                     DDPMNNAVELASLSDMSVPGPHNVANALAAAALARAFGVAPSAVAEGLRAFTPAPHRI
                     ALVAVIDGVQFVDDSKATSPHAAAASLTSFDSIVWIAGGLGKGIPFDDAVRVAAPRLR
                     AA"
BASE COUNT          398 a          888 c          935 g          532 t
ORIGIN      
        1 gacgtcgcgt ctgtgggtgc cggccttggt cgtgtacaga cggtcagccg atggcgcatg
       61 caggtctctg agcgatccga tggactcatc gtgatcaacg acgcctacaa cgccaaccct
      121 gagtcgacgc gagcggcgtt gcgggcgttg gcggccatgg gcgcgggaga tcgccagcgg
      181 cgcacacatg cggtgctggg tgtcatggcc gagttgggtg cggggtcggt tggtgcgcat
      241 cgcgagcttg gcgagacggc ggcacacctc ggcgtccatc gcctcgttgt tgtcggtgca
      301 ggtgcgcaag gcatcgcaga cggtgccctc gacgccggcc tcgacccgac gcgcgtcgtc
      361 gttgtcgatg acgttgccgc ggcgacggcg ttgctcgaat cagatctcgg gccggacgac
      421 gtcgtgctgg tgaaagcgtc acgctcggcc gacttgcagc gggtggcaga ggcgttgctc
      481 gccgcgcctg cacccgtggc tgccccgaac ggaggcggcc gatgaaaggc attctggccg
      541 cgggcaccat cgcgctggtc atctcgctgt tcggcacccc gtttgcgatc aggctgctca
      601 cgagccgcgg ttacgggcag ctgatccgcg acgacgggcc gacgacgcat cacaccaagc
      661 gcggcacgcc gaccatgggc ggcacggtca tcttgctggc cgcgatcctt ggctacgcgg
      721 ctggaaacat gctcagcgcc agcggcccta cggcatcggg attgctcgtg ctcggactcg
      781 cgaccgggct agggcttgtc gggttcctcg acgacttcat caaggtccgt atgcagcgca
      841 acctcggctt gcgggcccgc acgaagctgc ttgggcagac gttggtggcc gtcgtgttcg
      901 gcatcttggt cttgcagttt cccgacagct caggcattca tccggcgtcg acacacatct
      961 ccttcgtgcg cgacacgacc gttgctcttg gcgcggtcgg gttcgttatc tgggcattag
     1021 tgctcgtgtc gtcgacgtcg aacggggtga acctcaccga cggtcttgac ggcctcgcca
     1081 ccggagcatc gaccatggtg ttcgcggcat tcgtcatcat gacgttctgg caaggccgta
     1141 atgattgcct caccaaggct gccgctggct gctatccggt gcgagacccg ttcgacctgg
     1201 cgatcctcgc tgctgccttc gcgggagcat gtgcgggatt cctgtggtgg aatgccgcgc
     1261 ctgcacgcat tttcatggga gacaccggct cactcgcgct cggcggggcg ctcgccggga
     1321 tggccatcat gacccgcacc gagctgctcc ttgccgtgct cggcggactg ttcgtcgtca
     1381 tcacgatgtc gagcatcatc cagatcggct acttcaagat gacccaccgc cgggtgttca
     1441 aaatggcgcc gctgcaacac catttcgaac tgaaaggctg ggccgaggtg acgatcgtga
     1501 tccggttctg gattatcgcc gggcttgcgg tcgccctcgg cctcggcgtc ttctacgcag
     1561 agtggatcac gtggcagaag taaccgtgtc gtggttgccg tcggctgcat acgacgcacc
     1621 atggcacgag ttgaccgtgt gtgttgccgg cgtcggcgtc tccggtgccg cgtgcgcacg
     1681 cgtgctgcga tcactgggtg cagcggtcat cgctgtcgac gctggtgtcg gtgataggca
     1741 gcagcagacg gcagcagaac tcgcgcatat cggcgtcgat gtccgcctcg gcgacggcga
     1801 cacgttgccc gaagctgtgc agctcgtcgt gacgtcgcct gggtggcgac gcgactcacc
     1861 gctgttcatc gcggcggcat cgagtggcac ccccatttgg ggcgagccgg aacttgcgtg
     1921 gcggctacgc gagcccgacg cagccccgtg gctggtcgtc accggtaccg acggcaaaac
     1981 gacgacgacg ttgatgctcg agtcgatgtt gcatgccgcc ggacaccgcg cggcgtccgc
     2041 gggcaacatc ggcgcggctc tcgtcgagct cgtgaacggc ccctacgacg cgttggcggt
     2101 cgagctcggc agctttcagc tgcactggtc gccttcggtc gcaccgctcg ccgccgccgt
     2161 gctcaacgtc gcgcccgacc atctcgactg gtgggcagac tcgttcgagg actacgccgc
     2221 cgcgaagggt ctcgcgttcg ctcatgccga cacctgcgcg gtcggaaacg ccgacgacgc
     2281 ggtcagtacg cggctgctcg ctcgggcgcc gggacgtcga gtggcgttca ccttgaacag
     2341 tccccggccg catcaactcg gcgtggtcga agaccttttg gtggatcggg cctttgtcga
     2401 cgatccgatg aacaacgcgg tcgagctcgc gtcattgagt gacatgtccg tgcccggacc
     2461 gcataacgtc gcgaacgctc tcgccgctgc cgcgctggcg cgtgcgttcg gggttgcacc
     2521 gagtgccgtc gcggagggct tgcgcgcctt cactcctgcg ccgcatcgca tcgcccttgt
     2581 cgccgtgatc gacggggtgc agttcgtcga cgattcgaag gccaccagcc cgcatgctgc
     2641 ggcggcttcg ctgacgtcgt tcgattcgat cgtgtggatc gcaggcggcc tcggcaaggg
     2701 aattccgttc gatgatgccg tgcgtgtcgc agccccgcga ttgcgtgcgg ctg
//
LOCUS       DASWVF010000322         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_339992, whole genome shotgun sequence.
ACCESSION   DASWVF010000322 DASWVF010000000
VERSION     DASWVF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339992"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGD55_05720"
     CDS             <1..203
                     /locus_tag="VGD55_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0869872.1"
                     /translation="LGAFAVTLYPFDIQQQADGLYEALTMAPTRRRELREAAADIVRA
                     NDVGKWLAFQLADLSEITGMWS"
     gene            292..1314
                     /locus_tag="VGD55_05725"
     CDS             292..1314
                     /locus_tag="VGD55_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869873.1"
                     /translation="MPRVRFPLALLAAAVLLITGCASQTSTSARSVTSSPAPAPAGYA
                     CSTADLFTQPVDAAVDQHVTLGDTTAALTGTSESDGVEPTELRGGVLAITRAGDTTVA
                     LPITPPASSQIVDLFILETQSGRSNPDDETAGSLCLVRFSDGAAPVALIALTTGGAHC
                     CITVRAVPIDGSPTLDRDFGNYGPWLRNVSSNASVVPATVPVMVTADNAFAYQFTSFA
                     GSGPPVQLVSWTGTAFTDVTRRHLDVVRDDAQSYLAAYNDPTQPEKLGFLAGWVADEC
                     LAGDAGTAWTFYDQQWRLGHAIDVTDPAWAATANEAAMPQFVVAHGYCPASFGITASR
                     ASSPTK"
     gene            1375..3150
                     /locus_tag="VGD55_05730"
     CDS             1375..3150
                     /locus_tag="VGD55_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY0869874.1"
                     /translation="MSGATSTSVPSEAAATPSTTGAFTHRQVLTILTGLMLGMFLAAL
                     DQNIVATAIRTIGDDLHGLSVQAWVTTAYLITSTVSTPLYGKLSDLYGRKPFFLIALT
                     IFITGSAACAFSTSMYMLAAFRAYQGLGAGGLFSLALAIIGDIVSPRDRAKYQGYFLA
                     VFGTASVLGPVLGGFFAGSSSILTITGWRWVFLVNVPVGIVALYVVWRTLNLPNTHRP
                     ARIDWVGVASLFVCLVPLLVVAEQGQKWGWASGTSLACYALGIAGLVLFILAEHRMGD
                     DALIPLRFFQNRVFSLTSIVGVVVGMGLFGGISVLPLYLQIVRGVSPTEAGLLLIPLT
                     AGLMVGAIVSGQMISRTGHYKVFPIVGSGLCVVALVLMSFIGADTPLWQTDIYGLLFG
                     IGIGNIMQPITLATQNAMPARDIGVATSSATFFRQMGGTLGVAVFLSVLFSTAGGNIA
                     HAYKNAAKSASFQSLTTDPTVTNAAANKPLLSILNGQKLSSSSLNDTAFLKHANKQLA
                     HPFFVGFSNSMDVIFLLAAAVMLIGFLVLLAVEELPLRTMSGIQAQQADAKEALERTN
                     ADADARESGQDADTSGTSGVEPSPA"
     gene            complement(3099..>4158)
                     /locus_tag="VGD55_05735"
     CDS             complement(3099..>4158)
                     /locus_tag="VGD55_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0869875.1"
                     /translation="AVGAWAGLTGVASALGPVVGGVLVGGAPWGWRLVFLINLPLAVV
                     VVLAARHIPETRDESVDGHIDFVGAAFAATGLAAVIYGLTEGSSFGWGARQLGATALG
                     VVLLAVFAAVELHERTPMLPMRLFRSRQFTAANAVTVIVYGALGGVFFLVPVELQQGA
                     GFSPVEAGASLLPVTAMLLLLSARMGRIAARRGPRLQMAVGPILAATGVALLTRIHPG
                     VSYVPAVLPGVLVFGVGMAVTVAPLTATVMAAAPPGDVGVASAVNNDLARTAALFAVA
                     VLPALSGITALAYKSPTALSNGFHNAAWIAATLCALGGVLAGVFIRNDVLAGTTRGAV
                     PGQAGDGSTPEVPEVSAS"
BASE COUNT          711 a         1441 c         1299 g          707 t
ORIGIN      
        1 agctcggcgc gtttgcagtg accctgtatc cgttcgacat tcagcaacaa gcagacgggc
       61 tctacgaggc cctgacgatg gcgccgacgc gacgacgtga gttgcgtgag gccgccgccg
      121 acatcgtgcg cgcgaacgat gtcggcaaat ggctcgcgtt tcagcttgcc gatctgtcag
      181 agatcaccgg gatgtggtcg tagcgttcag cgtgtgcgga ttcagcagtc tctcttgacc
      241 ttcgaccggc cggtagggca accggtcgcg actgccgcgc gtctatggga cgtgccgcgc
      301 gttcgatttc cgcttgcctt gctagccgcg gccgtcctct tgatcaccgg ctgcgcatca
      361 caaacctcaa cctcggcccg ctcggtcacc tcgagccccg caccagctcc agccggttac
      421 gcctgtagca cggccgacct cttcacccag cccgtcgatg ccgcagtcga ccagcacgtc
      481 acgctcggtg acacgaccgc agccctgacc ggcacgagcg agagcgacgg agtcgaaccc
      541 accgagcttc gcggcggcgt tctcgcgatc acccgagccg gcgacaccac ggtggcactg
      601 ccgatcaccc cgcccgccag ctcacagatc gtcgacctct tcatcctcga aacacagagc
      661 gggcgttcca atccggacga cgaaactgcc ggctcgttat gcctggtcag gttcagcgac
      721 ggcgcggctc cggtcgcgct gatcgcccta acaacgggtg gcgcgcattg ctgcatcacc
      781 gtccgggcag tgccaatcga cggcagcccg acgctcgacc gtgactttgg caattacggt
      841 ccgtggctgc gcaacgtctc gagcaacgcg tctgtcgtcc ctgccacagt gccggtgatg
      901 gtcactgccg acaacgcctt cgcctaccag ttcacctcgt tcgccggctc aggtccgccg
      961 gtgcagctag tcagctggac cggcaccgcc ttcaccgatg tcactcgccg tcacctcgac
     1021 gtggtgcgcg acgacgcgca gtcatatctg gccgcgtaca acgacccgac gcaacccgag
     1081 aagctcggct ttctggctgg ctgggtcgcc gacgaatgcc tggccggcga cgcggggacc
     1141 gcgtggacgt tctatgacca gcagtggcgg ctcgggcacg ccatcgacgt gaccgacccg
     1201 gcgtgggcag cgaccgcgaa cgaggctgca atgccacagt tcgtcgtggc gcacgggtac
     1261 tgtccggcga gcttcggcat aaccgcctcg cgggccagtt cgccgacaaa gtagcgtgac
     1321 gtacagcggc gcccctagac aacgaggctg acgcgcacac ctgagggaga ttgcatgagc
     1381 ggagctacgt ccacatcggt gccgagcgag gcagcagcga ccccaagcac gaccggcgcg
     1441 ttcacccacc ggcaggtcct gacgatcctc accggcctca tgctcgggat gttcctcgct
     1501 gccctcgacc agaacatcgt cgcgaccgcg atccgaacga tcggcgacga cctgcacggc
     1561 ctctcggtac aggcgtgggt gacaaccgcg tatttgatca cgtcaacggt ctcgaccccg
     1621 ctgtacggca agctctccga cctctacggt cgcaagccgt tcttcctgat cgcgctcacg
     1681 atcttcatca ccggctcggc cgcctgtgcg ttctcgacct cgatgtacat gctggccgcg
     1741 ttccgcgcct accagggtct tggcgccggc ggcctcttct cactcgccct ggccatcatc
     1801 ggcgacatcg tctcgccgcg tgatcgggcc aagtatcagg gctacttcct cgccgtgttc
     1861 ggcacggcca gcgtattggg gcctgtgctc ggcgggttct tcgccggctc gagctcgatc
     1921 ctgacgatca ccggctggcg ctgggtcttc ctcgtcaacg tgccggtcgg catcgtcgcg
     1981 ctctacgtgg tgtggcgcac gctgaacctg cccaatacac accggcctgc gcgtatcgac
     2041 tgggtcggcg tggcgtcgct gtttgtgtgc ctggtaccgc tgctggtcgt cgccgaacag
     2101 ggtcagaagt ggggctgggc gtccgggacg tcgctcgctt gctacgcact cgggatcgcg
     2161 gggctggtgt tgttcattct tgccgagcac cgcatgggtg acgacgcgct gatcccgctg
     2221 cgcttcttcc agaaccgcgt gttcagcctg acctcgatcg tcggtgtcgt ggtcggcatg
     2281 gggttgttcg gcgggatctc ggtgttgccg ctctatctcc agatcgtgcg gggcgtgtca
     2341 ccgaccgaag ctggcttgtt gctgatcccc ctgaccgccg gtcttatggt cggcgcgatc
     2401 gtctcgggcc agatgatctc gcgcacaggc cactacaagg tcttcccgat cgtcggttcg
     2461 ggtctgtgcg tggtcgcgtt ggtcctgatg tcattcatcg gggccgacac gcccctgtgg
     2521 cagaccgaca tctacggttt gctcttcggc atcggcatcg gcaacatcat gcagccgatc
     2581 acgctggcca cccagaacgc gatgcccgcg cgcgacatcg gcgttgcgac gtcatcggcc
     2641 acgttcttcc ggcagatggg cggcacgctc ggagtcgcgg tcttcctgtc agtgctcttc
     2701 agtaccgccg gcgggaacat cgcgcacgcc tacaagaacg cagcgaagtc ggcgtcgttc
     2761 cagtcgctca cgaccgaccc gaccgtgacc aacgcagccg cgaacaagcc gctgctctcg
     2821 atcctgaacg gtcagaagct gtcgtcgtcg agcctgaacg acactgcctt cttgaagcac
     2881 gcgaacaagc aactcgcgca cccgttcttc gtcggattct ccaactcgat ggacgtcatc
     2941 ttcttgctcg ccgctgccgt catgctgatc ggcttcctcg tgctcctggc cgtcgaagaa
     3001 ctgccgctgc gcacgatgtc gggcatccag gcacagcagg ccgacgcaaa ggaagctctc
     3061 gagcgcacca acgccgacgc cgacgctcgc gaatcaggtc aggacgcaga cacttcgggc
     3121 acctcaggag tcgaaccgtc gccggcttga ccgggaacgg cacccctcgt cgtcccggct
     3181 agtacgtcgt tgcggatgaa gacgccggcg agcacgccac cgagggcgca cagcgtggcg
     3241 gcaatccacg ccgcgttatg gaagccgttc gacagcgcgg tcggtgactt gtaggcgagc
     3301 gcggtgattc ccgacagtgc cggcaagacg gcgacggcaa agagcgcggc ggtgcgggcg
     3361 agatcgttgt tgaccgccga tgcgacaccg acatcgccgg ggggtgccgc ggccatgacg
     3421 gtggcagtca gtggcgcgac agtcactgcc atgccaactc cgaatacgag gacgccgggc
     3481 agcacagccg gcacgtagct aacccctgga tgaatcctgg tgagcagagc gacgccggtg
     3541 gccgcgagga tcggaccgac cgccatctgc agccgcggtc cgcgccgggc cgcgatgcga
     3601 cccatgcgcg ccgacagcag caacagcatc gccgtgaccg gcaggagtga cgcgcccgcc
     3661 tccacagggg agaatccggc accctgctgt agttcgacgg gcaccaggaa gaacacgcca
     3721 ccgagtgcgc cgtacacgat gacggtcacg gcgtttgctg cagtgaactg acgtgaccgg
     3781 aacaggcgca tcggcaacat cggcgtccgc tcgtgcaact cgaccgctgc gaaaacggcg
     3841 agaagcacca cgcccaaagc cgtagcgcca agttgccgcg caccccagcc gaagctcgaa
     3901 ccttcggtga ggccatagat gactgccgcg agcccagtcg cggcaaaggc tgcgccgacg
     3961 aagtcgatgt gaccgtcgac cgactcgtca cgcgtctcgg gaatgtggcg ggccgccaac
     4021 acgacgacga ccgcgagcgg caggttgatc aagaacacga gtcgccatcc ccagggcgcg
     4081 ccccccacga ggacgccgcc aacgactggt ccgagcgccg acgcgacgcc ggtcagaccc
     4141 gcccacgcac ccacagct
//
LOCUS       DASWVF010000323         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_136415, whole genome shotgun sequence.
ACCESSION   DASWVF010000323 DASWVF010000000
VERSION     DASWVF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136415"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..453
                     /gene="arr"
                     /locus_tag="VGD55_05740"
     CDS             31..453
                     /gene="arr"
                     /locus_tag="VGD55_05740"
                     /inference="COORDINATES: protein motif:HMM:NF033144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEY0869876.1"
                     /translation="MPGPLDEGPFFHGTIADLRVGDFLTAGHHSNYRSEIVMNHIYFT
                     AVVAGAGLAAEIAAELAEGDAIPRVYAVEPTGAFENDPNVTDKKFPGNPTRSYRSRAP
                     LRIVSEITQWTRLTPEQLQTWRERLSALLSSDRGEIIN"
     gene            complement(809..1576)
                     /locus_tag="VGD55_05745"
     CDS             complement(809..1576)
                     /locus_tag="VGD55_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0869877.1"
                     /translation="MADGVETYRRSFARRSAAMAEADQTALDQPGICGVLGSAQFPGG
                     RVLITDDRAVPTLEALLPDLLVGILNVFEDASRCRELIEQAGRWSGQEAMAMICPDLH
                     TVPVLAVPAGLTIRTVRRSQTDAADGIPLEEAARACLAAEPDAGSDELPGFVEFLWSL
                     PPTTRLLAAVDDQNVVRATSGSSAFGVEANAYFVSTDEHWRGRGIATAMTASALAWAR
                     EAGASQASLDSSQAGLSIYLRLGFQPVSRARLFTRFS"
     gene            complement(1632..2387)
                     /locus_tag="VGD55_05750"
     CDS             complement(1632..2387)
                     /locus_tag="VGD55_05750"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0869878.1"
                     /translation="MDDPFLLAGETFSSRLIMGTGGIPSLDVLDAALEASGTELTTVA
                     MRRVEPAARGSILDVLARRGVRVLPNTAGCYTAADAVLTAQLAREALGTNWVKLEVIA
                     DEHTLLPDAVELLDAAEHLVADGFVVFPYTNDDPVLARRLEQVGCAAVMPLGSPIGSG
                     LGIRNPHNIELIVESAGVPVILDAGIGTASDAVLAMELGCDAVLLASAVTRAQHPALM
                     ADAMRRAVEAGRLARRAGRIPVRRYAKASSPPA"
     gene            complement(2392..2592)
                     /gene="thiS"
                     /locus_tag="VGD55_05755"
     CDS             complement(2392..2592)
                     /gene="thiS"
                     /locus_tag="VGD55_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0869879.1"
                     /translation="MTVVLNGERRELPDRATLADAVAQLTVAVRGVAVAVNGSVVPRG
                     LWPSTVLADSDDVEVLTAVQGG"
     gene            complement(2589..>3590)
                     /gene="thiO"
                     /locus_tag="VGD55_05760"
     CDS             complement(2589..>3590)
                     /gene="thiO"
                     /locus_tag="VGD55_05760"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY0869880.1"
                     /translation="AAGMLAPVTEVHYGEEDLLRLNLESAKRYPSFVAELEEFTGTPV
                     AYRASGTLAVAFDADDKAALDELHTYQSSLGLTSTPLTARECRQLEPFLATSIRGGLL
                     VEGDHQVDNRRLVDALLEACVRAGVEVRTTDAREITVDGDRVVGVDDIVAEVTVLAAG
                     CWSSVLHGLPLHALPPVRPVKGQIVRLRDNLAHPMLSRNVRAFVRGRPIYFVPRADGE
                     VVLGATSEEQGFDTRLTVEGIGDLLADGRELVPRVAEIELAEATVGLRPGSPDNTPMI
                     GAGALPGLVIATGHYRNGILLAPVTADAVADLLTSGALPQGFERFDPCRFARTAVPA"
BASE COUNT          650 a         1159 c         1167 g          614 t
ORIGIN      
        1 ggttcaacca ggtaggcaga aggatttcag gtgcctggac cgctcgatga gggtccgttc
       61 tttcacggca cgatcgcgga tttgcgtgtg ggtgacttcc tcaccgcggg ccaccattcg
      121 aactaccgta gcgagatcgt gatgaaccat atttacttca cagctgtcgt tgccggagcc
      181 ggcctcgctg cggagatcgc tgcagagctc gccgaaggtg acgcgatccc acgggtgtat
      241 gcggtagagc caaccggagc gttcgagaac gatccgaacg tgaccgacaa gaagttcccc
      301 gggaacccca ctcggtcgta ccgcagtcgc gccccactga gaatcgtcag tgagatcacc
      361 cagtggacgc gactgacacc cgagcagctt cagacgtggc gggagcgact ttcagcgctt
      421 ctttcaagcg accgtggcga gatcatcaat tgagctgcgc gtggaaccgc tgttcctacg
      481 ccacctcggt cacctggttg ggtccccctg ccagcggttt atgcgtctga cagttggtgt
      541 cgtaggccgt ggttcgtcat cgacggacgg ggccgcgtgc tggggtttcg acggcctccg
      601 aagcgagaat ggtgagccac cgcgtatcgg cccgagcgca ctgattgccg ttgcccacgt
      661 tcagctgacg cttcgccttt gccgcgacgc gctcatgagc attgccgcgg ccgtcgccgg
      721 ggcggtggga ccgcctagac ggccgcagcg gacagccgga accgtgtgtg tgcatggctt
      781 gttctaggtc ggcttggtgc ggcggcggct agctgaagcg ggtgaagagt cgggcacggc
      841 ttaccggctg gaagccgagg cggaggtata tcgacagccc ggcttggctg gagtcgagcg
      901 aggcctggga tgcgccggct tcgcgcgccc aggcgagggc cgaggcggtc atggccgttg
      961 cgatacctcg cccgcgccag tgttcgtcgg tgctgacgaa gtacgcgttg gcctcgactc
     1021 cgaatgcgct ggagccagag gtggctcgca cgacgttttg atcgtctacg gcagcgagga
     1081 gtcgtgtcgt tggcggtaag gaccacaaga actcgacgaa tcccggaagc tcatcactgc
     1141 cggcgtcggg ctcggccgcc aaacaggcgc gcgcagcttc ctcgagggga atcccgtcgg
     1201 cggcgtccgt ctgcgagcgg cggacggtgc ggatcgtgag gcccgcgggg acggccaaga
     1261 ccggcaccgt gtgaagatcc ggacagatca tcgccatggc ttcctggccg ctccaacggc
     1321 cggcctgttc gatgagctcc cgacaacgcg acgcgtcttc aaacacgttt aagatcccaa
     1381 ccaaaagatc tggcagcaag gcttccagtg tcgggaccgc acggtcgtcg gtaatcagca
     1441 cccggccacc aggaaactgt gccgaaccga gcaccccgca aatgccgggc tggtccagcg
     1501 cggtttgatc tgcctcggcc atcgccgcac tacggcgcgc gaacgacctg cgataggtct
     1561 caacaccatc tgccacttta ggcaccccca tcgtcgtcgc cttgtacatc ctgagtttcc
     1621 gcagcgccca cctacgcggg tggtgacgat gccttcgcgt agcgacgaac tggtatccgg
     1681 cccgcacgac gagccaaccg gccggcttcg accgcacgac gcatcgcatc agccatcagc
     1741 gccggatgct gcgcccgcgt gacggccgag gcaagcagca ccgcgtcaca tccgagttcc
     1801 atcgcaagga ccgcatcact cgccgttccg atgcctgcgt cgagaatcac tggaactcca
     1861 gcgctttcga cgatgagttc gatgttgtgc gggttgcgaa tcccaagccc cgagccgatc
     1921 ggcgaaccga gtggcattac tgccgcgcag ccgacctgtt cgaggcgacg tgcgagcacc
     1981 gggtcgtcgt tggtgtaagg gaaaacgacg aatccgtcgg ccaccaggtg ttctgcggca
     2041 tcgagcagtt cgaccgcatc aggcagcaac gtgtgctcgt cggcgatgac ctcgagcttc
     2101 acccaattcg taccgagtgc ctcacgcgcg agttgcgcgg tgagcactgc gtcggctgcg
     2161 gtatagcaac ccgcggtgtt cggtagcacg cgcactccac gccgcgccag cacatcgaga
     2221 atcgagccgc gagcagcagg ctcgacccgg cgcatcgcaa ccgtcgtgag ctcggtccct
     2281 gaagcctcga gtgcggcgtc gagcacgtcg aggctcggaa tcccgccggt gcccatgatg
     2341 agacgcgagc tgaatgtctc gccagcgagc aggaacggat cgtccacgat gtcagcctcc
     2401 ctgcaccgcg gtcagcactt cgacgtcgtc ggaatctgcg agcacggtcg agggccagag
     2461 cccgcgtggc acgacacttc cgttgacagc caccgcgaca ccgcgcacgg cgacggtcag
     2521 ttgagcaacc gcgtcggcca gcgtggcgcg atcgggtagt tcacgtcgtt cgccgttcag
     2581 caccaccgtc atgcgggcac cgccgtgcga gcgaaccggc aggggtcgaa ccgctcgaac
     2641 ccctgcggca aggcacccga tgtgagcagg tcggcgaccg catcggccgt caccggcgcg
     2701 agcaggatgc cgttgcggta atggccagtc gcgatgacga ggcccggcaa tgcgccggct
     2761 ccgatcatcg gggtgttatc gggggagcca ggccgtaagc ctacggtggc ttcggcgagt
     2821 tcgatttcag cgacgcgggg caccaattct ctaccgtcag ccaaaaggtc gccgatgccc
     2881 tcgaccgtga gcctggtatc gaagccctgc tcttcgctcg tcgcgccgag caccacctcg
     2941 ccgtcagcac gaggaacgaa atagatcggc cggccacgga cgaacgccct cacattgcgg
     3001 ctgagcatcg gatgcgcgag gttgtcgcgc agccgcacga tctgaccctt caccggccgc
     3061 acgggaggaa gggcgtgcag cggaagaccg tgcagtactg aagaccagca gcctgctgct
     3121 agcacggtca cctcggcgac aatgtcgtcg acgccgacga cgcggtcacc gtcgaccgtg
     3181 atctcacgtg cgtcagtcgt gcggacttct acacccgcgc gcacgcacgc ctctagcaac
     3241 gcgtcgacaa ggcgtcggtt gtcgacctga tggtcgccct cgacaagcag ccctccgcga
     3301 atgctggtcg cgaggaacgg ttcgagctgt cggcactcgc gagctgtcag cggtgtcgat
     3361 gtcaggccga gcgaagactg gtaggtgtgt agctcgtcga gtgcagcttt gtcgtcggca
     3421 tcgaaggcga cggccagcgt gccgctggca cggtaagcaa ccggcgtccc ggtgaattct
     3481 tcaagttctg caacgaaact tgggtacctc ttcgccgact cgaggttcaa gcgcagcagg
     3541 tcttcttcgc cgtagtgcac ctcagtaact ggagcgagca tgcccgcggc
//
LOCUS       DASWVF010000324         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_238208, whole genome shotgun sequence.
ACCESSION   DASWVF010000324 DASWVF010000000
VERSION     DASWVF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238208"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..393
                     /locus_tag="VGD55_05765"
     CDS             52..393
                     /locus_tag="VGD55_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869881.1"
                     /translation="MIEIKVSYDRDAEFALESCKPWAALGLTHDQKAGIDDPIEMERV
                     ADEHADQAHKRFIVTDDCDSVIEKVLPYVELGFEDLVLHAPGHDQKRFLDQFGADVLP
                     TLRQEAEKRVS"
     gene            483..>1958
                     /locus_tag="VGD55_05770"
     CDS             483..>1958
                     /locus_tag="VGD55_05770"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0869882.1"
                     /translation="MAVIVAMAAASASAAQLAVVAEHDTDHVSFVNTATYQLVGGPVA
                     AGEGPTSVAITPDAKYAYVTDSEGETVSVIETGLRHPIATIEKVGKEPFGIAITPDGK
                     YAYVTARGSNQVAVISTQTKEVVKSITVGVEPTGVAISPTGKFAYVANYADSDVEVIN
                     TETMTLVPGKPIKVGEGPMEIEFTPAGTAYVVDEIGKKVSAINTVNKEVTEIPLSSKS
                     GEPQGIAISPDGTEAYVVGLGAGPISVIDTGTNQVTGEVKVAGEPQEVAFAANGNTAY
                     VTENEPHQVQTIDVDTRQTAGSPIGLPGLSLTGIALTPDQSPVAAFTSPRATVGVPAP
                     FDGSASTDADGTIASYNWTVEDGRDATGVSAVHTFLTAGTFNAKLTVVDDEGCGEAMV
                     FTGRTAYCSGGASSVSHPVTVAAAPTAETQPPVCKSNFGVGGLTHNRKNGTAKLRVKL
                     RTAGSLLLFGKKVHAVTRKTKAAGSMTLTIHARVELNKRLKK"
BASE COUNT          412 a          661 c          608 g          277 t
ORIGIN      
        1 ctcaacgagg gcgccgagaa agccgagcgc gacccggcca agatccgcaa gatgatcgag
       61 atcaaggtct cctacgaccg cgacgccgag ttcgcgctcg agtcctgcaa gccgtgggcg
      121 gcgctgggac tgacccacga ccagaaagca gggatcgacg acccgatcga gatggaacgg
      181 gtcgccgacg aacacgccga tcaggcccac aaacgcttca tcgtcaccga cgactgcgac
      241 tcggtgatcg agaaggtcct cccctacgtc gaactcggat tcgaagacct cgtcctccac
      301 gcgcccggcc acgatcagaa gcgcttcctc gaccaatttg gcgccgacgt gctgccgacg
      361 ctgcgccaag aggccgaaaa gcgcgtctcc tgaaaccgag ccgcccctcg gctgcctaga
      421 atgaggcacc gatgggcagg tcggtaagag ttttgcgtca ttcagtggtc gttgcggcgg
      481 tcgtggccgt catcgtggcg atggccgcgg cgagcgcgag cgccgcacag ttggccgtag
      541 tcgcggaaca cgacacggac cacgtctcgt tcgtcaacac ggcgacgtat caactggtcg
      601 gtggaccggt cgcggccggt gaaggcccga cctcggtcgc gatcaccccc gatgccaagt
      661 acgcctacgt caccgactcc gaaggcgaaa cggtctcggt gatcgaaacc ggtctgcggc
      721 atccgatcgc gacgatcgaa aaagtcggca aagagccttt cggcatcgcg atcacccccg
      781 acggcaagta cgcctacgtc accgcccgcg gcagtaacca agtggcggtg atctcgactc
      841 agaccaaaga ggtcgtgaag tcgatcacgg tcggcgtcga acccaccggt gtggcgatct
      901 cgccgacggg caagttcgcc tacgtcgcga actacgccga cagtgacgtc gaagtgatca
      961 acacggaaac gatgacgctg gttcccggca aaccgatcaa agtcggcgaa ggcccgatgg
     1021 agatcgaatt cactcccgct gggaccgctt acgtcgtcga tgaaataggc aaaaaagtct
     1081 cggcgatcaa taccgtcaac aaagaagtca ccgaaatccc gctctcgtcg aaaagcggcg
     1141 aaccgcaagg gatcgcgatc tcccccgacg ggaccgaagc gtacgtagtc ggcctcggtg
     1201 ccgggccgat ctcggtgatc gacacgggca cgaaccaagt caccggcgaa gtcaaagtcg
     1261 ccggcgaacc acaggaagtc gcgttcgccg caaacggcaa caccgcctac gtcaccgaga
     1321 acgaaccgca tcaggtgcag acgatcgacg tcgacacgcg ccagaccgcc ggctcgccga
     1381 tcggcctgcc tggcttgtcg ctgaccggca tcgcgctgac cccggaccag tcgccggtcg
     1441 ccgccttcac gtcgccgcgc gcgacggttg gcgtcccggc gcccttcgac ggctcggcct
     1501 cgaccgacgc cgacggcacg atcgcctcct acaactggac cgtcgaagac ggtcgcgacg
     1561 cgaccggtgt cagcgccgtc cacaccttcc tcaccgccgg caccttcaac gcgaagctga
     1621 ccgtcgtcga cgacgaaggc tgtggcgaag cgatggtgtt caccgggcgc acggcctact
     1681 gcagcggggg cgcgtcgtcg gtgtcgcatc cggtcacggt cgccgcggcg ccgacggccg
     1741 aaacccagcc tccggtctgc aagagcaact tcggcgtcgg tggcctcacc cacaaccgca
     1801 agaacggcac ggcgaagctg cgggtgaagc tgcgcacggc tggatcgctg cttctcttcg
     1861 gcaagaaggt ccacgcggtc acgcgcaaaa cgaaggcggc cgggtcgatg acgctgacga
     1921 tccacgcccg ggtcgagctg aacaaacggc tgaagaag
//
LOCUS       DASWVF010000325         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_212858, whole genome shotgun sequence.
ACCESSION   DASWVF010000325 DASWVF010000000
VERSION     DASWVF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212858"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VGD55_05775"
     CDS             <1..1010
                     /locus_tag="VGD55_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HEY0869883.1"
                     /translation="QGARRKLERVFSRQEFRRAFPGEIADLTNPSRTLEDYSQEVMRC
                     VDTTGVLDAGLKVVIDTAGGTAALVLPTLLGRIGIDVLTVNNRLDESSPTETVAEHMR
                     ALERLGELVASSRAAFGVRFDPVGERISLVDERGVIVHDDRALLVLMDLVAAELHTGS
                     IAIPITTTRIAEDVARFHHVTTLWAPTSPDELTKAAGSTEAIFAGDGRGGYVIPEFNT
                     AIDGIAAFVRLIGLVARTKLTLSQIDARIPQAHVLRRSVPTPWAAKGMVMRSVIEAAG
                     DRELDTTDGVRVVEDDGRWVLVLPDPAEATTHLWAEGPDADSATELLEEWAAVVEGAG
                     T"
     gene            1067..1972
                     /locus_tag="VGD55_05780"
     CDS             1067..1972
                     /locus_tag="VGD55_05780"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY0869884.1"
                     /translation="MSLLLDLMANAVDQGYADAAKRKAGRVRPETPAQRTAPAVAPAA
                     PPTEPVSATAAAPATGLTRWVPTRTTAAAAVVLALAGALFATAAVETHRGNAAAQRDR
                     RQLVQQAKLQTTAVDGLQQQLAALRTQVNDARTRALSVNDRGAVLLNQIKGLEPVDGV
                     VAVAGTGVRIVLDDSPASRAAGSDGSGVILDSDLQLAVNGLFLAGAQAVSINGQRLTT
                     QTAIREAGGAILVDYRPLSPPYQIDAIGPMSLGATFTSGQTAQLFVTEHQLYGLGFTL
                     QDHERLTLPSAANVVVHYAQPLDAQ"
     gene            1969..>2171
                     /locus_tag="VGD55_05785"
     CDS             1969..>2171
                     /locus_tag="VGD55_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HEY0869885.1"
                     /translation="MIAALALAVGVVLGVILHPTVPVGLQPYLPIAVVAALDAVFGAI
                     RAILDGIFDDKVFVVSFIANVLVA"
BASE COUNT          355 a          751 c          705 g          360 t
ORIGIN      
        1 cccagggggc ccggcgcaag cttgagcggg tgttttcacg ccaggagttc cggcgcgcgt
       61 tccccggtga gatagccgac ctgaccaatc cgtcgcgcac cctcgaggac tactcccaag
      121 aggtcatgcg atgcgtcgac acgaccggcg tcctcgatgc gggtctgaag gtggtcatcg
      181 acacagcggg tggcactgcc gcactcgtgt tgcctaccct gctcggtcgc atcgggatcg
      241 acgtcctgac cgtgaacaac cggctcgacg agagttcgcc cacggagacg gtcgccgagc
      301 acatgcgggc attggagcgg ctcggcgaac tcgtcgcctc gtcacgtgcg gctttcggag
      361 tgcgcttcga tccagtggga gaacggattt cgctggtcga cgagcgcggc gtgatcgtgc
      421 acgacgaccg tgcgctactc gtgttgatgg acctcgtcgc cgccgaactc cacaccggca
      481 gcatcgcgat cccgattacg acgactcgca tcgccgaaga cgtcgctcgc ttccaccacg
      541 tgaccacact ctgggcaccg acatcgcccg acgaactgac caaagcggcc ggctcaaccg
      601 aggccatctt cgccggcgat ggcaggggcg gctacgtcat ccccgagttc aacacggcta
      661 tcgacggcat cgccgcgttc gtgcggctca tcggcctggt cgccagaacc aagctgaccc
      721 tgagccagat cgacgcacgc attccccagg cgcatgttct acgtcgctcc gttcccacac
      781 cgtgggccgc gaagggcatg gtcatgcgat cggtgatcga agccgcgggc gatcgcgaac
      841 tcgacacgac cgatggcgtt cgcgtggtcg aagacgacgg ccgttgggta ctcgtgctgc
      901 ccgacccggc cgaagccacc actcacctgt gggcggaggg ccccgacgcc gactccgcca
      961 ctgaactgct cgaagagtgg gccgcggtgg tcgagggggc cggcacctga cgacctcgtc
     1021 aacgcgcgcg tccgaaagcc cggccgaccg tcgtcccgac gcttcgatgt ccttgttgct
     1081 cgacctgatg gcgaacgccg tcgaccaggg ctacgccgac gccgcgaagc gcaaagctgg
     1141 gcgtgtccgt cctgagacgc cagcgcagcg aacggcgccg gccgtggcac cggctgcccc
     1201 gccgaccgag ccggtgagtg ctacggctgc cgcgccggcc accggactca cccgctgggt
     1261 ccccacccgc acaaccgccg cggcggcagt ggttcttgcg ctggcaggtg cgttgttcgc
     1321 caccgcggcg gtcgagacgc atcgcggcaa cgcggcggcc cagcgtgacc gccggcagtt
     1381 ggtgcagcag gcgaagctcc agacgacggc ggtcgacggg ctccaacagc agctcgctgc
     1441 acttcgaacc caggtcaacg acgcccgtac gcgcgcactc tcagtcaacg atcgcggggc
     1501 ggtgctgctg aaccagatca aaggcctcga gccggtcgac ggcgtggtcg cggtggcggg
     1561 tacgggcgtg cgcatcgtgc tcgacgactc accggcatct cgagccgccg gtagcgacgg
     1621 gtctggtgtg atcctcgaca gcgatctcca actcgcggtc aacggtctgt tcttagcagg
     1681 tgcacaagcg gtgagtatca atggccaacg gctcacgaca cagacggcga tccgtgaggc
     1741 gggtggtgcg atcctggtcg actaccgccc gctgtcgccg ccttaccaga tcgatgcgat
     1801 cggtccgatg agcctcgggg cgacgttcac gtccgggcag acggcgcagc tgttcgtgac
     1861 cgagcatcag ctctacggcc tggggttcac cctccaggat catgaacgcc ttacgcttcc
     1921 ctcggcggcc aacgtggtcg tccactacgc ccagccgttg gatgcgcaat gattgccgcc
     1981 ctcgccctcg ccgtcggtgt cgtgctcggc gtcatcttgc atccgaccgt accggtcgga
     2041 ttgcagccgt atctgccgat cgcagtggtg gccgcgctcg acgcagtgtt cggcgcgatc
     2101 cgcgcaatcc tcgacggaat ctttgacgac aaggtcttcg tggtgtcgtt catcgcgaac
     2161 gtcctcgtcg c
//
LOCUS       DASWVF010000326         4739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_314379, whole genome shotgun sequence.
ACCESSION   DASWVF010000326 DASWVF010000000
VERSION     DASWVF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314379"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1084)
                     /locus_tag="VGD55_05790"
     CDS             complement(98..1084)
                     /locus_tag="VGD55_05790"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0869886.1"
                     /translation="MGAPVPIARALNMGLRKALESDPKVMIMGEDVGKMGGVFRVTDG
                     LQKDFGEDRVMDTPLAESGIIGTAIGLAMRGYRPICEIQFDGFVFPAFDQIVSQLAKL
                     HYRSQGRVAMPVVIRIPFGGGIGAVEHHSESPEALFAHIAGLKVVACSNAPDAYTMIQ
                     QAVASNDPIIFFEPKRRYWEKAEIDVDTPLAEAVGLHKARVVRPGRHLTLVAYGPMVK
                     TCLDVARVAADEGHELEVVDLRSLSPVDWPTLTDSVRHTGRCVVVHEAQTFLGMGAEL
                     AARITEQCFYELQAPVMRVGGYPTPYPPSRLEEHFLPDLDRVLDMVDRSLAY"
     gene            complement(1084..2187)
                     /gene="pdhA"
                     /locus_tag="VGD55_05795"
     CDS             complement(1084..2187)
                     /gene="pdhA"
                     /locus_tag="VGD55_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY0869887.1"
                     /translation="MTADPGTGERDLVQLLTPEGQRLEHPDYPLEVTADEMRGFYRDL
                     SLVRRFDLEATALQRQGELGIWAPSLGQEAAQIGSGRALLPGDMAFPTYREHGVAWCR
                     GVEPEALLELFRGVNFGGWDPKEHNFALYTIVIGAQTLHATGYAMGMQRDGAEDAVIA
                     YFGDGASSEGDVNEAFIFAASYNAPVVFFLQNNQWAISVPEERQSRIPLFRRAHGFGF
                     PGVRIDGNDVLACLAVTRKALQYAREGQGPFLIEAFTYRMGAHTTSDDPTRYRLASEL
                     ENWKLRDPIERVKAYLSRNGLADAAYYEAIDAEAAALAVRVRTACLSMPEPNPAEIFK
                     QVYAGGSPLVDEERAAFDNYQAGYEPATVGSGQ"
     gene            complement(2327..3061)
                     /locus_tag="VGD55_05800"
     CDS             complement(2327..3061)
                     /locus_tag="VGD55_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869888.1"
                     /translation="MKLGAALRLPPALAATPLKTLVAAISLVGITTAATVSVIVIGSN
                     AAVSPPSHQPVAGSNFGSPTQQGASPLIVVPKQRKPSTTGVPTPSVNPTPTVTGSSTA
                     PTPAVTPTTVPTPPTSPISSPPAPVTKPKPPKPVTVTVDVPLPVVVPPVVTPPAVVPP
                     TVTPPPVVPPVVVPPVVKPPKPPKPTPPPCHRPPPPPCHKPPGSGDKDPKPKPPDGGQ
                     HPCPHPTPPPCPHPHAPPPPCAHHSH"
     gene            complement(3469..4563)
                     /gene="hisC"
                     /locus_tag="VGD55_05805"
     CDS             complement(3469..4563)
                     /gene="hisC"
                     /locus_tag="VGD55_05805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0869889.1"
                     /translation="MADEQTPRARADLAGIAVYKPGKPSAVGFKSYKLSSNESPYPPL
                     PSVLEAIAHAAQQANRYPDLAASIVVNALAAKLEVPADHVVVGCGSVGVATQLVEAFA
                     GPGDEVVFAWRSFEAYPIIVQVSGATAVPVPLKAGAVHDLAAMADAVTPRTRVVFVCN
                     PNNPTGTAVRRGELEAFLDQVPGDCLVVLDEAYREFNRAPDVPNGVSIYGDRPNVAVL
                     RTFSKAYGLAGLRVGYAVAHPPVADAIRMTSVPFAVSSIAQAAAVASLLPPAEAELFA
                     RVEATVGERARVSEVLAKVGWEVPESEANFVWLATGSSTDKAAARCAEAGIVVRPFSG
                     EGLRVTIGEPEANDIFLAVAADLQADVPKA"
BASE COUNT          784 a         1537 c         1642 g          776 t
ORIGIN      
        1 gccgacgggc cagtgcacga tctcggcctc ggtcagacct tcgccgacgt cgggaagctt
       61 gaactgcttg agcgatgcca caccagcctc cagcggttca gtacgcgagt gagcggtcga
      121 ccatgtcgag cactcgatcg aggtcgggca ggaaatgctc ctccagccgc gacggcgggt
      181 agggggtcgg gtacccgccg actcgcatca caggagcttg cagctcatag aagcactgtt
      241 cggtgatgcg cgccgcgagt tcggcgccca tcccgagaaa cgtctgggcc tcgtggacga
      301 cgacacatcg gccggtgtga cgcaccgagt cggtcagtgt cggccaatcg accggtgaca
      361 gcgacctgag gtcgacgacc tccagctcgt gcccctcgtc ggcagccacc cgcgccacat
      421 cgaggcaggt cttcaccatc ggcccgtagg cgaccaatgt cagatgccgg cccggcctga
      481 ccacgcgcgc tttgtgcagc ccgacggcct ctgcaagagg tgtgtcgacg tcgatctcgg
      541 ccttctccca gtaacgccgc ttgggctcga agaaaatgat cggatcgttg ctcgcgaccg
      601 cctgctggat catcgtgtac gcgtcgggcg cgttcgaaca ggcgacgacc ttgagaccgg
      661 cgatgtgggc gaacaacgct tcgggcgatt cgctgtggtg ctcgaccgcg ccgatgccac
      721 caccgaatgg gatgcggatc acgaccggca tcgcgacccg gccctgcgag cggtagtgca
      781 acttggccag ctggctgacg atctggtcga acgccgggaa cacgaacccg tcgaactgga
      841 tctcacagat cggccggtag ccgcgcatcg ctagcccgat cgcggtgccg atgatgcccg
      901 actcggcgag cggagtgtcc atcacgcggt cttcgccgaa gtctttttgg agaccgtcgg
      961 tcacccggaa tacgccgccc atcttgccta cgtcttcgcc catgatcatg accttgggat
     1021 ccgactcgag cgccttgcgc agacccatgt tcagtgcccg cgcgatgggt accggcgcgc
     1081 ccattactga ccgctcccga ccgtcgccgg ctcgtagccc gcctggtaat tgtcgaaagc
     1141 agcgcgttct tcgtcgacaa gcggagagcc gccggcgtag acctgcttga agatttcagc
     1201 cgggttcggt tcgggcatcg acaagcacgc cgtgcgcacg cgaacagcca gcgcagcggc
     1261 ctccgcgtcg atggcttcgt agtacgcggc gtcggccagc ccgttgcgtg acaggtacgc
     1321 cttcacgcgt tcgatggggt cgcgcagctt ccagttctcg agctcgctcg cgagccggta
     1381 gcgcgtcgga tcgtccgacg tcgtgtgtgc gcccatgcga tacgtgaacg cctcgatgag
     1441 gaacggtccc tgaccttcgc gcgcatactg gagtgccttg cgtgtgacgg cgaggcaggc
     1501 cagcacgtcg ttgccgtcga tccgcacgcc ggggaagccg aacccgtgcg cccgccggaa
     1561 caacgggatc cgcgactgcc gctcttctgg caccgagatc gcccactggt tgttctgcaa
     1621 gaagaagacc acgggcgcgt tgtacgacgc tgcgaagatg aatgcctcgt tcacgtcgcc
     1681 ttcacttgac gccccgtcgc cgaagtaggc gatgaccgcg tcttcggcgc catcgcgctg
     1741 catgcccatc gcatatccgg ttgcgtgcag cgtctgcgca ccgatgacga tcgtgtagag
     1801 agcgaagttg tgttcctttg gatcccagcc gccgaagttc accccgcgaa aaagttcgag
     1861 cagcgcctcg ggctcgacac cgcggcacca ggcgacgccg tgctcgcggt aggtcggaaa
     1921 cgccatgtcg ccagggagta acgcgcgccc tgagccgatc tgcgcggcct cttgcccaag
     1981 cgacggtgcc cagatgccga gctcgccctg tcgctggagt gccgtcgcct cgagatcaaa
     2041 acggcgcacg agcgagaggt cgcggtagaa gccgcgcatc tcatcagcgg tgacctcgag
     2101 cgggtagtca ggatgctcga gccgttgccc ctcaggggtc aaaagctgca ccaggtcgcg
     2161 ctcgcccgta ccggggtcgg ccgtcatcgt gtggcactcc tcctgctcgc cgtcgcgagg
     2221 gtccctcgcg acgggcgccg gtcacgggat cagccggtgg gcatcgtccg tgccaggtac
     2281 cgggtaatcg tcgcagatcg tgagcgggtc gtgggtatcg ctttggtcag tggctgtggt
     2341 gcgcgcacgg cggcgggggc gcgtggggat gcgggcaagg aggcggtgtc gggtgcgggc
     2401 aaggatgttg cccgccgtcg ggtggcttcg gtttggggtc cttgtcaccc gacccgggag
     2461 gcttgtggca cggcggaggt ggcggccggt ggcacggggg cggcgtcggc ttaggtggct
     2521 tcggcggctt gaccacgggc ggcacaacga caggggggac gacgggtggc ggggtcaccg
     2581 tgggcgggac gaccgcaggc ggagtgacga cgggaggcac cacaaccggc agcgggacgt
     2641 cgacggtgac cgtgaccggc ttaggcggct ttggcttggt gacgggcgcg ggcggactgg
     2701 aaatgggtga ggtgggtggt gtcggcacgg tggtcggggt gacggccggc gtcggcgccg
     2761 tgctggaacc ggtcacggtc ggcgtcgggt tcaccgaggg tgtcggcacc ccagtcgtag
     2821 agggcttgcg ttgcttggga accacgatga gcgggcttgc gccctgctgg gtgggtgagc
     2881 cgaagttcga gccggctact ggctgatggg acggcggcga caccgctgcg ttcgagccga
     2941 tgacgatgac gctgacggtc gcggccgtgg tgatgccaac caacgagata gccgcgacca
     3001 gagtcttcag cggggtcgcc gccagcgctg ggggcagtcg caacgcagcc ccgagtttca
     3061 cggcagcgac acctccctaa cagtggcgga caggtccttt ggcgagcgcc gtggctcccg
     3121 ataactactc tatacggacg gtaccgacta agtcactcag cgttgcaagt gtggccgatt
     3181 ggcctagtga caccggttcg tatcgacaca aatcagccga acacaagcgc cttccgcgag
     3241 tcgccggact cgaatttgag cgtgccgaga cctgcgagcc tcgtggagat cgggccggcc
     3301 gccgtcgcgg ccacgggcct ctggggagtc cacgagatgg tgcccctgag cttagacata
     3361 aggaggcgcc gcaaccatcg ccaacgcggg gggcttgccg atagctcaga tggcgcctgc
     3421 gccgagtcgt tggacagggc caaagggggc tgcgcccgag ccgaaaggtc aggcctttgg
     3481 cacgtcggcc tggaggtcgg ccgcgaccgc gagaaagatg tcgttggcct ccggttcgcc
     3541 gatggtgacg cgcagaccct caccggagaa tgggcgcacc acgatgccgg cctccgcgca
     3601 cctggcggct gccttgtcag tactcgagcc cgtcgccagc cagacgaagt ttgcctcact
     3661 ttctgggacc tcccaaccga ccttcgcgag cacctcgctg acccgcgcgc gttcaccgac
     3721 cgtggcctcg acccgagcga acagctcggc ttcggcgggg ggcagcagtg acgcgaccgc
     3781 ggcggcttgg gcgatcgaac tgacggcgaa gggcacgctc gtcatgcgga tcgcgtctgc
     3841 caccggcggg tgcgcgaccg catagccgac gcggagcccg gccaacccgt aggccttcga
     3901 gaaggtccgg agcacggcga cgttgggccg gtcaccgtag atcgaaacac cgttcggtac
     3961 gtcgggagcg cggttgaact cccggtaggc ctcgtcaaga acgacgaggc agtcgcccgg
     4021 cacctggtcg aggaaggcct cgagctcgcc ccgacgaacc gcggtccctg tcggattgtt
     4081 tgggttgcag acaaacacca cgcgggtccg tggcgtgacc gcgtcagcca tcgcggcgag
     4141 gtcgtgcacg gcgcccgcct tcagcggcac cgggacagcg gtggcgccgg agacctggac
     4201 gatgatcggg taggcctcga acgatcgcca cgcgaacacc acctcatcgc ctggaccagc
     4261 gaaggcctcg acgagttggg tggctacgcc gacagatccg caaccgacga cgacgtggtc
     4321 ggccggaacc tcgagtttgg ccgcaagtgc gttaacgacg atggacgccg cgaggtcggg
     4381 gtatcgattc gcttgctgag ccgcgtgggc aatcgcctcg agaacgctcg gcagcggcgg
     4441 atacggcgat tcgttcgacg acaacttata gctcttgaag ccgaccgccg agggcttgcc
     4501 tggtttgtag acagcgatac cggcgagatc ggcccgggcc cgcggcgtct gctcgtcagc
     4561 cactgctacc tccgacactc ccgcacgttt gcacgccgca accctacggc ggccagggtc
     4621 gcggacccca acccgttatc gccccccgcg cgacactttg gcgcgcccgt cggcgtcccg
     4681 ctaccctgct ccggtacagc aggggacccg aaagggtccc gggaggcttc gcctagtcc
//
LOCUS       DASWVF010000327         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_416671, whole genome shotgun sequence.
ACCESSION   DASWVF010000327 DASWVF010000000
VERSION     DASWVF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416671"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGD55_05810"
     CDS             <1..461
                     /locus_tag="VGD55_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869890.1"
                     /translation="TVTGLNAAGGNDVFDTWGGTTTIVDSYLHDPTRNVAGTSHDDDF
                     QLTGGTATLTHDTLIAPIAAQANSCIQLGDLSGSSNGVTLANSLCDGGNYSINANGTA
                     VTSGTVTCGQLVFTGDRFGTDYRYGVKTNLGAPFTTTWSGNVDDTTGNPV"
     gene            complement(573..1670)
                     /locus_tag="VGD55_05815"
     CDS             complement(573..1670)
                     /locus_tag="VGD55_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0869891.1"
                     /translation="MNPRARRSFLRGRRLGHVRALAVIGGSLALTIGVSASAQADTIN
                     QANAQAKKLAAQIEKLQPKADAALAAYDTSLAALGQAVSTNVAAGRIYSSLLAQADNL
                     VTQQDSHIVALYKSGGSLALYASALRAGDPSGLVQLPYLKAIVTTDAEQSAAALRLAD
                     AAKATLAASQTQIDNGLANADSVNVRLAAVQSILAQQEGLLDEASGRAKNLQALKDAA
                     AALAASQAAADQAGAQAAADAQPYPIPPLYRALYMNAATTCPGLSWTVLAAIGQVETH
                     HGSGGMVSSAGALGPMQFLPSTFVEYARDGDHDGLANIMDPADAIYSAAAYLCANGAG
                     QGGQSLYTAIWNYNHADWYVQLVLALSGKIT"
     gene            1763..2536
                     /locus_tag="VGD55_05820"
     CDS             1763..2536
                     /locus_tag="VGD55_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0869892.1"
                     /translation="MPTLTPWEIAGVLGAGVVAGTVNTVVGSGSLLTFPTLLAFGYSP
                     LVANVSNTLGLVPGAASGALGYRRELAGQGSRIRRLGAFSIAGGLLGAGLLLMLPSTS
                     FERVVPYLIVLACVLVVLQPRLSARMIRRAAERPDVPRPHALLDATVFLTAIYGGYFG
                     AAQGVILIALLGIFVDDHLQRLNAAKNVLAALVNGVAALLFIIFAHIAYLPAGLLAVG
                     SAIGGQIGARVGRRLAPDLLRLLVVVVGIGVAVKLFVSP"
     gene            complement(2555..2767)
                     /locus_tag="VGD55_05825"
     CDS             complement(2555..2767)
                     /locus_tag="VGD55_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869893.1"
                     /translation="MDDLQVIGSRGRGSVMRIVRASAALFGSGFVAGLVVALIFHALI
                     HALIIIAAVLIVAGFAIKVMVTRRRS"
     gene            2787..>3503
                     /locus_tag="VGD55_05830"
     CDS             2787..>3503
                     /locus_tag="VGD55_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0869894.1"
                     /translation="MIAAVVINTSTVRQVAASWMMRCVARTVFIDGVTDPRLADYRAL
                     TDVSLRRRIEPEQGIFIAEGELVIRRALRAGYPMRSALMTERWLSTMVDGGALADLVA
                     ETDVPLYVGSEELLESVTGFHVHRGALAAMGRLAPLGAGELVQRLPARARIVVLEDVN
                     SHTNLGAIFRCAAGLGMDAVLLSPSCTDPLYRRSVRVSMGEVFAIPYARFEKWPEGLS
                     EITDAGFHTLALTPDPNAESI"
BASE COUNT          560 a         1139 c         1155 g          649 t
ORIGIN      
        1 cgaccgtcac tgggctgaac gcggctggtg gcaacgacgt gttcgacacg tggggtggca
       61 cgaccaccat cgttgactcc tacctgcacg acccgacgcg caacgtcgcc ggcacttccc
      121 acgacgacga cttccagctc accgggggga ctgcgacgct gacccacgac accctgatcg
      181 cacccatcgc agcccaagcc aactcctgca tccagctcgg tgacctgtca ggttcatcga
      241 acggtgtcac tttggcgaac agcttgtgtg acggcgggaa ctactcgatc aacgccaacg
      301 gcactgccgt cacatccggc accgtgacgt gcgggcagct ggtgttcacc ggcgaccggt
      361 tcggcaccga ctaccgctat ggagtcaaaa ccaacctcgg tgcaccattc acaaccacct
      421 ggtcaggcaa cgtcgacgac accaccggca accccgtcta accggcgaac gcgcgctgtg
      481 cgtcggtgcg ttcagctgaa cgggcgtggt ggcagaaccg gtcgcgttcc taccaacccg
      541 tcacgaaggc agttgcgcgt tcacgtccac ggctacgtga tcttgcctga cagagctagg
      601 actagctgca cgtaccagtc agcgtggttg tagttccaga tcgcggtgta aagcgactgg
      661 ccaccttgac ccgcaccgtt ggcgcacagg taggcggcgg cgctgtagat cgcgtcggcc
      721 ggatccatga tgttggcgag gccgtcgtgg tcgccgtctc tggcgtattc gacgaatgtc
      781 gacggtaaga actgcatcgg gccaagtgcg cccgctgacg agaccatgcc accgctgccg
      841 tggtgcgtct cgacctgacc gatcgcggcc aggacggtcc aagacaatcc cgggcacgtt
      901 gtcgcggcat tcatgtacag cgcccgatac agcggcggga tcggataggg ctgcgcgtcg
      961 gcggccgctt gcgccccagc ctgatcggcg gccgcttgcg acgcggcgag ggccgcggcg
     1021 gcgtccttga gagcttggag attcttggcc cggccggacg cttcgtcgag caacccctcc
     1081 tgctgggcga gaatgctctg cacggccgcg agtcggacgt tgacggagtc ggcgttggcg
     1141 aggccgttgt cgatctgggt ctggctggcg gcaagggtcg ccttcgccgc atcggcgaga
     1201 cgcaacgcgg ccgccgattg ctcggcgtcg gtggtcacga tcgccttcag atagggcaac
     1261 tgcacgagac cggacgggtc acccgcgcgc agcgccgagg cgtagagagc gagcgatcca
     1321 ccgctcttgt agagcgcgac gatgtgggag tcctgttggg taacgaggtt gtcggcctgc
     1381 gccaggagtg agctgtagat gcgcccggct gcgacgttgg tcgagaccgc ttggccgagc
     1441 gctgcaagcg aggtgtcata ggccgccagc gcggcgtccg ccttgggttg aagcttctcg
     1501 atctgtgccg cgagcttctt cgcctgggca ttggcctggt tgatggtgtc ggcctgggcg
     1561 gatgcgctca ccccgatcgt cagcgccagc gagcctccga tcactgctag agcgcgaacg
     1621 tggcccagtc ggcgtccgcg caggaaggag cgccgtgccc gggggttcac cttaggtacg
     1681 tcgaccgttg ccgaccctgc cttgaccctt acggacgatg gtgaccgatc tctccccgcg
     1741 aacaccgggc tagcatcggg gcgtgccgac gctgaccccg tgggagatcg ccggcgttct
     1801 gggagccggc gttgttgcgg ggacggtgaa taccgtcgtc ggatccggct cgttgctgac
     1861 ctttccgacg ctgctcgcgt tcggctattc gcctctggtc gccaacgtct caaacacgct
     1921 cggcctcgtc cccggtgcgg caagtggggc cctcggatac cgccgcgagc tcgctggtca
     1981 gggttcgcgg attcgccggc tcggtgcgtt ctccatagcc ggcggattac ttggggccgg
     2041 cttgctgctc atgcttccga gtacgtcgtt cgaacgcgtg gtgccctacc tcatcgtgct
     2101 tgcgtgcgtg ctggtcgtgt tgcaaccgcg gttgtcggcc cgcatgatcc ggcgtgctgc
     2161 tgagcgaccc gacgtcccac gcccgcacgc gctgcttgac gccacggtgt tcttgaccgc
     2221 gatctacggc ggctatttcg gtgcggcgca aggcgtgatt ctgatcgcac tgctcggcat
     2281 cttcgtcgac gatcatctac aacggttgaa cgccgccaag aacgtgctcg cggcgctcgt
     2341 caacggagtg gctgcgctct tgttcatcat cttcgcgcac atcgcctact tgccggccgg
     2401 gttgctcgcg gtcgggtcgg cgatcggagg gcaaatagga gcaagggtcg gacgccgatt
     2461 ggcgcccgat ctcctcaggt tgctcgtcgt cgtggtcggg atcggcgttg cggtcaagct
     2521 cttcgtctcg ccgtagcgtg cgcccgcaaa agcgtcacga acgccggcgg gtcaccatga
     2581 ccttgatggc aaagccagcc acgatcagaa cggccgcgat gatgatcagc gcgtgaatca
     2641 gtgcgtggaa aatcagggcc accacgaggc ctgcgacaaa gccgctgccg aacaacgcgg
     2701 cgctcgcgcg cacaatgcgc atcaccgacc ctcggccacg gctgccgata acttgcaggt
     2761 cgtccataga actcaagctc cctcaggtga tcgccgcagt ggtgatcaac acctccacgg
     2821 tacgccaggt ggccgcgtcg tggatgatgc gatgcgtggc ccgcacggtg ttcatcgacg
     2881 gcgtaaccga cccgcggctg gccgactacc gcgccctcac cgacgtcagc ttgcggcggc
     2941 gcatcgagcc ggagcagggc atcttcatcg ccgagggcga attagtgatt cggcgggcct
     3001 tgcgcgccgg ctacccgatg cggtcggcgc tgatgacgga gcggtggctc tcgacgatgg
     3061 tcgacggcgg cgcgttggcc gaccttgtcg ccgagaccga cgtccccctg tacgtcggct
     3121 ccgaggagtt gctcgagtcg gtcaccggat tccacgtgca ccgtggggcg ttggccgcca
     3181 tgggtcgcct cgcgccgctc ggtgcaggtg agctcgtgca gcgtttgccg gctcgtgcac
     3241 gcatcgtcgt cctcgaagat gtgaacagcc acaccaatct cggagcgatt ttccgttgcg
     3301 cagcagggct tggcatggac gccgtgttgc tcagcccgag ctgtaccgac ccgttgtacc
     3361 ggcgttctgt gcgggtctcg atgggggagg tgttcgccat tccctatgca cgctttgaaa
     3421 agtggcccga ggggttaagc gaaatcaccg acgcgggatt tcacacactc gcgctcaccc
     3481 ccgacccgaa cgctgaatcg atc
//
LOCUS       DASWVF010000328         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_59554, whole genome shotgun sequence.
ACCESSION   DASWVF010000328 DASWVF010000000
VERSION     DASWVF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59554"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGD55_05835"
     CDS             complement(<1..225)
                     /locus_tag="VGD55_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869895.1"
                     /translation="MFEFRKGIVKGVLEDVVAGAQRPEVVIAGAPVGEPARVVDVAPG
                     RRSGTAEEDATLIAKLDMTNQCRAWSVRVLV"
     gene            268..468
                     /locus_tag="VGD55_05840"
     CDS             268..468
                     /locus_tag="VGD55_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869896.1"
                     /translation="MPTGHSYTRTPPAIASPIKDEVVGSKEPLPEMTVPLEELEWEPL
                     PSDLQTPDPPRSEPSGSDPPPF"
     gene            complement(505..1077)
                     /locus_tag="VGD55_05845"
     CDS             complement(505..1077)
                     /locus_tag="VGD55_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEY0869897.1"
                     /translation="MLSRDARLLEKFLQAPFTYPDIGATASHPSSEPAGYHHVQRSEP
                     IGHGIAHFAAACDVLMSWGMHRGAGLRVTASAARAETDAVVVMRLRLGGSGVGVGSGV
                     GVGVVVPCRVVYVVNEERRVGFAYGTLPGHPESGEEAFVVELDDDEQVRLHITAFSRP
                     ARWFSKLGGPVGRRAQSLFTDRYVRALRRT"
     gene            complement(1142..2587)
                     /gene="ahcY"
                     /locus_tag="VGD55_05850"
     CDS             complement(1142..2587)
                     /gene="ahcY"
                     /locus_tag="VGD55_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0869898.1"
                     /translation="MTFDFKVADLGLADFGRKEISLAEHEMPGLMAMRAQHGASKPLT
                     GARITGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQDHAAAAMAVGPTGSVDAPA
                     GVPVFAWKGETLDEYWWSTEQALLWPDGPDGTGPNMLLDDGGDATLLVHKGVEFEKAG
                     EVPDPSTADSEEFEVVLRLLQRSLTEDPRRWTTIASGLRGVTEETTTGVHRLYQMQES
                     GSLLFPAINVNDSVTKSKFDNKYGCRHSLIDGINRATDVLIGGKVAVICGYGDVGKGC
                     AESLRGQGARVIITEIDPICALQAAMDGYQVATLDDVVATADIFVTATGCLNVITADQ
                     MSRMKHQAIVGNIGHFDNEIDIAGLSKLPGVKRVNIKPQVDEWVFANGNGIIVLSEGR
                     LLNLGNATGHPSFVMSNSFTNQVLAQIELYTKTAQYPVGVYVLPKHLDEAVARLHLSA
                     LGVRLTELTPAQAGYLGVPIEGPYKSDAYRY"
     gene            complement(2584..>3386)
                     /locus_tag="VGD55_05855"
     CDS             complement(2584..>3386)
                     /locus_tag="VGD55_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0869899.1"
                     /translation="REHPFGYGSERYFYAFVVSLVLFTVGGVFALYEGISKIAHPHHV
                     DHAVIAVVVLAVAVGLESLSFRTAVIESSKIKGAQSWVSFVRRTKNPELPVVLLEDTA
                     ALFGLAFALAGVGLSVLTGNPRWDGVGTLAIGALLVTVAIVLSIETKSLLIGESATPE
                     IEARIATALMAGPEVVAVIHMRTLHLSPDELLVAAKIAVAHDDTAAEVARGIDAAERR
                     VRAAVEIARVIYLEPDLARPPTILGAGDQDTHEESTHDQATDEQGDIR"
BASE COUNT          646 a         1155 c         1001 g          584 t
ORIGIN      
        1 caccaagaca cgtaccgacc aagcgcggca ttggttcgtc atgtcgagtt tcgcgatcaa
       61 agttgcgtct tcctcggctg ttcccgaccg gcggcccggt gccacatcga ccactcgtgc
      121 cggttccccg acgggcgcac ctgcgatgac aacctcgggc cgctgtgccc ccgccaccac
      181 atcttcaagc acgcccttga cgatgccctt gcgaaactca aacatcgcac cttgcagcaa
      241 ccatcagccg gcaccttcat ttggaccatg cccaccggac acagctacac ccgcacaccg
      301 ccagcaatcg catccccgat caaggacgag gtggtgggaa gcaaggaacc gctacccgaa
      361 atgacggtcc cactcgagga gctcgagtgg gagccgctgc cctctgatct gcagacgcct
      421 gacccaccgc gttccgagcc atcaggatct gatccgccgc cattctgatg gtcaactgtc
      481 gatcacgatc cccgtggtca tcgatcaagt gcgtcgcagg gctcgcacgt agcgatcagt
      541 aaacagtgac tgcgcccgcc tgcccactgg accaccgagt ttgctgaacc agcgcgccgg
      601 ccgcgagaaa gcagtgatgt gaagtcgcac ctgctcgtca tcatcgagct ccaccacgaa
      661 cgcctcttcg ccgctctcgg gatgaccggg cagcgttccg tacgcgaagc cgacgcggcg
      721 ttcctcgttg acgacgtaca ccactcggca cgggacgacg acaccgacac caacaccaga
      781 accgacaccg acaccagacc cgcccaggcg caaccgcatc accaccactg cgtcggtctc
      841 agcgcgggca gctgaagctg tcacgcgcag gcccgctccg cggtgcattc cccaggacat
      901 gagcacatcg caagccgcgg cgaaatgcgc aatgccgtga ccaatgggtt cggaccgctg
      961 cacgtggtga tagcccgccg gctcggagct tggatgcgac gccgtcgcgc cgatatcggg
     1021 gtaggtgaag ggcgcctgca gaaacttctc aagcaaacga gcatcacggc tcagcacgga
     1081 atcgcttgct cgctcatgaa gaagtctgcc cgtccgcaga cgcagccggt ggccgaaccg
     1141 ctcagtagcg gtacgcgtcg gacttgtaag ggccttcgat cgggacgccg aggtaacccg
     1201 cctgtgctgg cgtcagctct gtcagccgca cgccgagcgc cgacaggtgc agccgcgcaa
     1261 cggcctcgtc gaggtgcttg ggcagcacgt acaccccaac tgggtactgc gcggtcttcg
     1321 tgtacagctc gatctgcgcc agcacttggt tggtgaacga gttgctcatc acgaagctcg
     1381 ggtggcctgt tgcgttgcca aggttcagca accgcccctc ggacagcacg atgatcccgt
     1441 tcccattagc gaatacccac tcgtcgacct gcggcttgat gttgacgcgc ttcacgccag
     1501 gcagcttcga caagccagcg atgtcgatct cgttgtcgaa gtgaccgatg ttgccgacga
     1561 tcgcctggtg cttcatccgc gacatctggt cggccgtgat gacgttgagg cagccggtcg
     1621 cggtgacgaa gatgtcggcg gtcgcgacga cgtcgtcgag cgtggccacc tggtagccat
     1681 ccatagcagc ctgcagtgcg cagatcgggt cgatctcggt gatgatgacg cgcgcgccct
     1741 ggccacgcaa tgattcggcg caacccttgc ccacgtcgcc atagccgcag atcaccgcga
     1801 ccttgccgcc gatcaacaca tcggtcgcgc ggtttatgcc gtcgatcagc gagtgccggc
     1861 agccgtactt gttgtcgaac ttcgacttcg ttaccgagtc gttcacgttg atggccggga
     1921 acagcagcga gccggactcc tgcatctggt acagccggtg cacgccggtc gtggtctctt
     1981 ccgtcacgcc gcgaagaccg gacgcgatcg tcgtccagcg tcgtgggtct tcggtcaatg
     2041 accgctggag caaccggagc acgacctcga actcttcgga gtcggcagtc gacgggtcgg
     2101 gcacctcgcc cgccttctcg aactcgacac ccttgtgcac gagcaacgtc gcgtcgccac
     2161 cgtcgtctaa tagcatgttc gggccagtac cgtcggggcc atcgggccac aacagcgcct
     2221 gctcggtgct ccaccaatac tcgtcgaggg tctcgccctt ccacgcgaac acaggcaccc
     2281 cggccggcgc atcgacgctg ccggtcgggc cgaccgccat ggccgccgcc gcatgatcct
     2341 gtgtcgagaa gatgttgcag ctcgcccacc gcacctgcgc acctagggca acaagcgtct
     2401 cgatcaggac ggcggtttgc accgtcatat gcaacgagcc ggtgatccgc gcgccggtca
     2461 acggcttgct cgcaccatgc tgggcccgca tcgccatgag gccgggcatc tcgtgttcgg
     2521 cgagggaaat ctccttgcgt ccgaagtcgg ccagcccaag atcggcaacc ttgaagtcga
     2581 acgtcatcga atatctccct gctcatcagt ggcttggtcg tgtgtggatt cctcgtgcgt
     2641 gtcttggtcg cccgcaccaa ggatagtcgg tggccgggcg agatctggct caaggtagat
     2701 cacccgtgcg atttctaccg ccgcccgcac ccggcgctcg gccgcgtcga tgccgcgagc
     2761 aacctcggcc gcagtgtcgt cgtgagcgac cgcgatcttc gccgccacaa gaagttcgtc
     2821 aggactcagg tgcagggtgc gcatgtggat cacagcgacg acctcaggtc cggccatcaa
     2881 cgccgtggcg atccgggcct cgatctcggg cgtcgccgac tcaccgatca acaggctctt
     2941 cgtttcgatc gacaaaacga tggcaacggt gacgagtagg gctccgatcg cgagggtgcc
     3001 gacgccgtcc catcgcggat tgccggtcag caccgaaagc ccgaccccgg cgagggcgaa
     3061 cgcaaggccg aacagggccg cggtgtcttc gaggagcacc actggtagct cagggttctt
     3121 tgtccttcgc acaaaactca cccatgactg ggcgcccttg atcttgctcg actcgatgac
     3181 cgcagtgcga aacgacaacg actccagccc gacagcgacg gccagcacca cgacggcgat
     3241 aaccgcatgg tcgacgtgat gcgggtgcgc aatcttgctg atgccttcgt acagcgcgaa
     3301 cacaccgccg accgtgaaca aaacgagcga gaccacgaat gcgtagaagt accgctccga
     3361 cccgtagccg aacggatgct ctcgat
//
LOCUS       DASWVF010000329         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_161575, whole genome shotgun sequence.
ACCESSION   DASWVF010000329 DASWVF010000000
VERSION     DASWVF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161575"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VGD55_05860"
     CDS             <1..1052
                     /locus_tag="VGD55_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869900.1"
                     /translation="ENEETHWPASYGGRKNDSGDDPDVIANYPGRIGFTDANSSPEPN
                     DLNEHGGIYAHLLRNGLDFVNFGNGYEFALVDEDGQTEPTGIREHANVPMEKIVRDRT
                     DHLYPEFNTHIPDAPLPEDPDRFNRFGRFKQVFEAHYVDRATGACKLPSYVDLYYPND
                     HGGGANDIHPNGPTWDAQRYFQDNDAALGLTVDLISKSPCWKDTVIFVVEDDTQNGLD
                     HVDGYRSLFLAISPWVKREHVAKGHLSLASIFKTVDLIFGIPPLNQYDAAATDLRELF
                     TNRPDFAPYDFVQPTYVAMGKPSWKRLTRHIDFSKPDRDEVALRRAIQQSEGLPRHQP
                     PMRHGRAPRSRHSAK"
     gene            1505..>1903
                     /locus_tag="VGD55_05865"
     CDS             1505..>1903
                     /locus_tag="VGD55_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869901.1"
                     /translation="MESIAHRALTSRRFAPCLISLAMLGEGCAALTSSGAVAPMRAEN
                     ATQLAASFAYAYGPATVTVNRVTLQGNADMQASTLGTPPPLPSPFPTLGVRQSLGPNA
                     EVGADIGIMDSGLRLRVGEPEGSSLPCDVAF"
BASE COUNT          342 a          654 c          610 g          297 t
ORIGIN      
        1 ccgagaacga ggagacgcac tggccggcct cgtacggcgg ccgaaagaac gactcgggcg
       61 acgaccccga cgtgatcgcc aactacccgg gccgcatcgg cttcaccgac gcgaacagct
      121 cgcccgaacc caacgacctc aacgagcacg gtggcatcta cgcgcacctg ctgcgcaacg
      181 gcctcgactt cgtcaacttc ggcaacggtt acgagttcgc gctggtcgac gaggacgggc
      241 agacggagcc gaccggcatc cgcgagcacg cgaacgtgcc gatggagaag atcgtccgtg
      301 atcgcaccga ccatctctac cccgagttca acacccacat ccccgacgcg cctttgcctg
      361 aagacccgga tcgtttcaac cggttcggtc ggttcaagca ggtgtttgaa gcgcactacg
      421 tggatcgggc caccggcgcg tgcaagctgc cgagctacgt cgatctctat tacccgaacg
      481 accacggtgg tggcgcgaac gacattcacc cgaacggccc gacctgggac gcgcagcgct
      541 actttcagga caacgacgcg gcgctgggat tgaccgtcga cctcatctcg aagagcccgt
      601 gttggaagga caccgtgatt ttcgtggtcg aggacgacac gcagaacggc ctggatcacg
      661 tcgacgggta tcggtcgctg ttcttggcga tttccccctg ggtgaagcgg gagcacgtcg
      721 cgaagggtca cttgagcctc gcgtcgatct tcaagacggt cgatctcatc ttcggcatcc
      781 cgccgctcaa ccagtacgac gccgcggcga ccgatctgcg cgagctcttc acgaaccgcc
      841 cggacttcgc gccctacgac ttcgtgcagc cgacctacgt ggcgatgggc aagccgagct
      901 ggaagcgtct gacgcgccac atcgacttct ccaaacccga tcgggacgag gtggcgctgc
      961 gtcgcgcgat ccagcagtcg gaggggctgc cgcgacacca gccgcccatg cgccacggcc
     1021 gcgcgccgcg gtcgaggcac agcgcgaaat gaggggaggg gtcctcagag ctcgaggagg
     1081 ccgttgtcag ccggcacctg tgttccgccc acgacgatca ggccgctgtt ggtcgggacc
     1141 gcgactccga ggggagcgat cgatggtgac ccggcacagc tgaccggaga ccaggcgccg
     1201 gcgggaatcg gcggaccacg atctcggtcc ccgtccagac gccgtactcc gcgacgggcg
     1261 gcgcgccgtc gaaggatatc gaccgtcacg ttccgacccc ggcggcgctc gggcgaccca
     1321 caacccgacc acgaaaaccg acgagcgcat caccgcatta tcgcgatttc acgcgatgtt
     1381 gccgcgatcg gtgtcacctc ggaaggtgtt cgaattctga cggtcgcgac ggtcgaagca
     1441 tcggcgccaa gccctttgca agttggcctc tgggttgctg ttagcaggcc gcaagggccg
     1501 acccgtggaa tccatcgctc accgcgcgct cacgtcgcgc cgattcgcgc catgcctgat
     1561 ctcgctcgcg atgttgggcg aagggtgcgc cgcgctcacg tcgtccggcg ccgtcgcccc
     1621 gatgcgcgcg gaaaatgcga cgcagctcgc ggcttcgttt gcatacgcgt acggacccgc
     1681 gacggtgacg gtcaatcggg tcaccctgca gggcaacgcc gacatgcagg cttcgaccct
     1741 cggcacgccg ccgcccctac cgagcccttt cccgacgctc ggcgtccggc agtcgctcgg
     1801 gccgaacgcc gaggtcgggg ccgacatcgg aatcatggat tcggggctcc ggctgcgcgt
     1861 gggagagccc gaaggatcgt cattgccgtg cgacgttgcg ttc
//
LOCUS       DASWVF010000330         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_238307, whole genome shotgun sequence.
ACCESSION   DASWVF010000330 DASWVF010000000
VERSION     DASWVF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238307"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGD55_05870"
     CDS             <1..234
                     /locus_tag="VGD55_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0869902.1"
                     /translation="EAAYAGKGYGDFKTDLADVVVEFVLPVQEKVRHYLDDTAALDAI
                     LAEGAERARGVAVATMKLVRERVGFLPLPPGKG"
     gene            240..779
                     /locus_tag="VGD55_05875"
     CDS             240..779
                     /locus_tag="VGD55_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY0869903.1"
                     /translation="MSRAAHTIGVAIAVPEPYGAELTKWRETLGDPLALAIPPHVTLI
                     TPMQVDDDQLALVIDHLDLVASGQWAFDIQLRGTGSFRPVSPVVFVALAAGISDCERV
                     EQAVRSGPLFHPLKFNYHPHVTVAHDVPPDVLDRAFDELADYEALFAVLGFGLFERGD
                     DGVWRTEREFPFRSSSPSA"
     gene            complement(754..2031)
                     /locus_tag="VGD55_05880"
     CDS             complement(754..2031)
                     /locus_tag="VGD55_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0869904.1"
                     /translation="MSSHLRRVVTPLAVAAVLALGALATFPGTAAADDVIGGAELGQP
                     GIVVGAGAAVPPLPATTATSFVVADADTGEVLAAHNAHEKLAPASTLKALTALTILPL
                     LPPNLPTVAQSDAPSVDGTKAGIVAGTTYTVDNLYTAMLMMSANDAAVSLADANGGLP
                     GTLAQMNTLAAHLQADDTVALTPDGLDAKGQSSSAYDLALIFRAGLTVPQFLYYLSLK
                     TAQFPAPKGQSFQIQTHDRLLTSYAGMLGGKNGYTVAAQASYVGAARRNGHTIIVSVM
                     RDQANFWPEVQALLNWGFAADGQATPVGDLVDPLPLAPLPSPSVAQAAEAAPTIAPAA
                     SAAPATATVAAKARRKSAGSATGISITLIWTAVAVVGLFAGAAWLQLSTRRRRAMETD
                     ERYLHGLHGLSRLSALDDFSSDNKNVKQTASST"
     gene            2124..>2576
                     /locus_tag="VGD55_05885"
     CDS             2124..>2576
                     /locus_tag="VGD55_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0869905.1"
                     /translation="MAGQTESGLPIEPVYDSSALDGFDPATALGAPGAFPYTRGVYPT
                     MYTGRLWTMRQYAGFGSAAQSNHRYRQLLEAGTTGLSVAFDLPTQMGYDSDDPMAAGE
                     VGKVGVAIDSIDDMRTLFDAIPLDKVSTSMTINAPAALLLLMYQLVAEE"
BASE COUNT          400 a          819 c          902 g          455 t
ORIGIN      
        1 gaggcggcgt acgcggggaa gggctacggt gacttcaaga ccgacctcgc agatgtcgtc
       61 gtagagttcg tgctgccggt gcaggagaaa gtccggcatt acctcgacga caccgcagct
      121 ctcgacgcga ttctcgcgga gggtgccgag cgggcacggg gcgtcgctgt cgcgacgatg
      181 aagttggtcc gcgaacgcgt cgggttcctg ccattaccgc cagggaaggg ctgagcttct
      241 tgagccgtgc cgcacacacg atcggtgttg cgatcgcggt gcccgagccc tatggcgccg
      301 aactcaccaa atggcgcgag acgctgggcg atccgctggc cttggccata ccaccgcacg
      361 tcaccctgat aacgccgatg caggtcgacg acgaccagtt ggcgttggtc atcgaccacc
      421 tcgacctcgt tgcgtcaggg cagtgggcgt tcgacatcca gctgcgcggc accgggagtt
      481 ttcggccggt gtcgcccgtc gttttcgttg cactcgcggc cggcatcagt gactgcgagc
      541 gcgtcgaaca ggcggtacgc agtggcccgc tcttccatcc gctcaagttc aactaccacc
      601 cacacgtgac ggttgcccac gacgtcccgc ctgatgttct tgatcgcgcg ttcgatgagt
      661 tggctgacta cgaggcgctg ttcgccgttc tcggatttgg tctgttcgaa cgtggtgacg
      721 acggcgtgtg gcgcaccgag cgggagtttc cttttaggtc gagctcgccg tctgcttgac
      781 gttcttgttg tcggacgaga agtcgtcgag tgccgagagc cgcgagagcc cgtgcagccc
      841 gtggaggtag cgctcgtcgg tctccattgc ccggcggcga cgcgtcgaca actggagcca
      901 cgctgcaccg gcgaagaggc cgacgaccgc gaccgcggtc cagatcagcg tgatcgagat
      961 tccggttgct gaccccgcac tcttgcggcg cgctttggca gccaccgtcg ccgttgcggg
     1021 tgcagctgac gctgccggcg cgattgtcgg cgcggcctcg gcggcttgcg ccacagacgg
     1081 gctcggcagc ggcgcgagcg ggagcggatc gacgaggtca ccgaccggcg tcgcctgccc
     1141 gtcggctgcg aatccccagt tgagcagggc ttgcacctcg ggccagaagt tcgcctggtc
     1201 gcgcatgacc gacacgatga tggtgtgacc gttgcggcgc gcggcgccga cgtagctcgc
     1261 ctgggcggcg accgtgtagc cgttcttgcc gccgagcatg ccggcgtacg acgtcagcaa
     1321 ccgatcgtgc gtctggatct ggaagctctg gcccttgggc gccggaaact gggccgtctt
     1381 taacgagagg tagtacaaga actgcggaac cgtgagcccg gcgcgaaaga tcaaggcaag
     1441 gtcgtaggcg gacgacgatt ggcccttcgc gtcgaggccg tcgggcgtga gtgccacggt
     1501 gtcgtcggct tggaggtggg cggcgagcgt gttcatctgc gcgagagtgc cgggtaggcc
     1561 gccgttcgcg tcggccagcg agaccgcggc gtcgttggcc gacatcatca acatcgctgt
     1621 gtagaggttg tcgacggtgt aggtcgtgcc ggccacgatc ccggccttcg tgccgtcgac
     1681 gctcggtgca tcgctttggg caacggtcgg caggttcggc gggagcagcg gcaggatcgt
     1741 gagcgcagtc agggccttga gcgtgctcgc cggggccagc ttctcgtgcg cgttgtgcgc
     1801 ggccagcacc tcgccagtgt cggcgtcggc caccacgaac gaggtcgcgg tcgtggccgg
     1861 cagtggcggc accgccgcgc ccgcacctac gacaatgccg ggctgaccga gttcggcgcc
     1921 gccgatgacg tcgtcagccg ccgccgtccc ggggaaggtc gccaacgcgc cgagagcgag
     1981 caccgcggcc actgccaggg gcgtgacgac ccggcgcaga tgggagctca tgaccgggca
     2041 cattacctgc cacatcagcc cacggctctg ctcggtcgat gcactgcccc tcccggctcc
     2101 ccgcgcagga acatactggg gcgatggcgg gtcagacgga atcgggactc ccgatcgagc
     2161 cggtctatga cagttcagcg ctggacggct tcgatccggc taccgcgctc ggggcgcccg
     2221 gcgcgttccc ctacacgcgc ggtgtgtatc cgaccatgta caccggccgg ttgtggacga
     2281 tgcggcagta cgcgggattc ggcagtgccg cacagtcgaa tcaccgctac cgacaactgc
     2341 tagaggccgg gacgacgggc ctgtccgtcg cgttcgatct gccgacgcag atgggctacg
     2401 actcagacga tccgatggct gcgggcgagg ttggcaaggt gggagtcgcg atcgattcga
     2461 tcgacgacat gcggacgctt ttcgacgcga tccccctcga caaggtgtcg acctcgatga
     2521 ccatcaatgc tccggccgcc ttgctgttgc tgatgtatca gttggttgcc gaggag
//
LOCUS       DASWVF010000331         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_492995, whole genome shotgun sequence.
ACCESSION   DASWVF010000331 DASWVF010000000
VERSION     DASWVF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492995"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGD55_05890"
     CDS             complement(<1..328)
                     /locus_tag="VGD55_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869906.1"
                     /translation="MLDVNFFDELRIGLATADDIRQWSHGEVKKPETINYRTLRPEKD
                     GLFCEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
                     HIWYFKG"
     gene            complement(389..>2713)
                     /locus_tag="VGD55_05895"
     CDS             complement(389..>2713)
                     /locus_tag="VGD55_05895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0869907.1"
                     /translation="ERMTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNP
                     LAGLTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLATH
                     GRINAFGFVETPYRRAVKGKVTDDVDYLTADEEDRFVIAQANAPLKDDGHFAEDRVLV
                     RRKGGEVDFVTPAAVDYMDVSPRQMVSVATAMIPFLEHDDANRALMGSNMQRQSVPLL
                     KSEAPLVGTGMEFRAAVDAGDVVTSAKDGVVEEVSSDAITVMNDDGTRTTYRMAKFRR
                     SNQGTCINQRPIVSEGQRVQAGAAIADGPCTDQGEMALGRNLLVAFMPWEGHNYEDAI
                     ILSQRLVQDDVLSSIHIEEHEVDARDTKLGPAEITRDIPNVSEEVLADLDERGIIRIG
                     ADVVPGDVLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIDVK
                     VFTRDDAELPPGVNELVRVYVAQKRKITDGDKLAGRHGNKGVISKILPQEDMPFLEDG
                     TPVDIVLNPLGVPGRMNVGQVLETHLGWAAKSGWKVDASGKDGWQQRLADIGALEAPP
                     NTLVATPVFDGAREDEITGLLGSTLPNADGQRPVGENGKARLWDGRSGELYPEAVSVG
                     YIYILKLHHLVDDKLHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYA
                     LQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVLSSDG
                     MTLEMRDTDDEVFRAAEELGIDLSRREPSSVEEV"
BASE COUNT          446 a          879 c          866 g          522 t
ORIGIN      
        1 cacccttgaa gtaccaaatg tgggtgactg gggcggccag ctcgatgtga cccatccgct
       61 cacgacgcac cttggcgcga gtgacctcga cgccgcagcg ctcgcagatg atgcccttga
      121 agcggacgcg cttgtacttc ccgcagtagc actcccagtc gcgggtggga ccgaagatct
      181 tctcgcagaa cagaccgtcc ttctcaggac gaagcgtgcg gtagttgatc gtctccggct
      241 tctttacctc gccgtgagac cactgacgga tgtcgtcagc agtggcgagg ccgatacgca
      301 actcatcgaa gaagttgacg tcaagcacag gttgtcttct ctctcctggt aaccgtcgaa
      361 cagggggccg tctgaacctt tactgcgctc agacctcttc gacgctgctc ggctcccgtc
      421 gagacagatc gataccgagc tcttccgcgg cgcggaagac ctcatcgtcg gtgtcgcgca
      481 tctccaacgt catgccgtcg ctggacagca cctcgacgtt gaggcacagc gactgcattt
      541 ccttgatcag caccttgaac gactcgggga tgccgggctc gggaatgttc tcgcccttga
      601 cgatcgcctc gtagaccttg acccggccga ggacgtcgtc ggacttgatc gtgagcagct
      661 cttgcagcgc gtaagccgcg ccgtaagcct cgagcgccca cacttccatc tcaccaaacc
      721 gctgaccgcc gaactgcgcc ttaccgccca gcggctgctg ggtgatcatc gagtaaggac
      781 cggtcgaacg tgcgtgaagc ttgtcgtcga cgaggtggtg cagcttcagg atgtagatgt
      841 atccgacgct gaccgcctcg ggatacagct cgccgcttcg cccgtcccac aaccgcgcct
      901 tgccgttctc gcccaccggg cgctgcccgt cggcgttcgg cagggtcgag ccgagcagtc
      961 cggtgatttc gtcttcacgg gcaccgtcga agacgggcgt cgcgaccaac gtgttcggcg
     1021 gagcttcgag cgcgccgatg tcagcgagcc gctgctgcca gccgtccttg ccgctggcgt
     1081 cgaccttcca accgctcttc gcggcccagc caaggtgcgt ctcgagtacc tggccgacgt
     1141 tcatgcggcc gggcacgccg agcgggttga gcacgatgtc gaccggggtg ccgtcttcga
     1201 ggaaaggcat gtcctcttgc ggcaggatct tggagatgac gcccttgttg ccgtgccgac
     1261 cggccagctt gtcgccgtcg gtgatcttgc gcttctgcgc gacgtagacc cgcaccaact
     1321 cgttcacacc cggcggcagc tcggcgtcgt cgcgggtgaa caccttgacg tcgatcacct
     1381 tgcccgactc accgtggggc accttcagcg atgtgtcgcg aacctcgcgg gccttctcgc
     1441 cgaagatcgc gcgcagcaac cgctcttcgg gggtgagttc ggtctcgccc ttgggcgtga
     1501 ccttgccaac aagaacgtcg cctgggacga cgtcggcgcc gatgcggatg atgccgcgct
     1561 cgtcgaggtc agcaagaact tcctcgctga cgttcgggat gtcgcgggtg atctctgcag
     1621 ggccgagctt ggtgtcacgc gcatcgacct cgtgctcctc gatgtggatc gaggagagca
     1681 cgtcatcttg cacgagacgc tggctcaaga tgatcgcgtc ttcgtagttg tgaccctccc
     1741 atggcatgaa cgccacgagc aggttgcgac caagcgccat ctcgccctgg tcagtgcacg
     1801 gaccgtcggc gatcgccgca ccggcctgaa cgcgctgccc ctcgctgaca atcgggcgct
     1861 ggttgatgca cgtgccctgg ttggaacgac ggaacttcgc catgcggtag gtggtgcggg
     1921 tgccgtcgtc gttcatgacg gtgatcgcgt cggaggagac ctcttcgaca acaccgtcct
     1981 tggccgatgt cacgacatcg cccgcgtcga cggcggcacg gaactccatg ccggtaccga
     2041 cgagcggcgc ttcggacttc aagagcggca ccgactggcg ctgcatgttc gaacccatga
     2101 gtgcgcggtt cgcgtcgtcg tgctcgagga acgggatcat cgcggtcgcg accgacacca
     2161 tctggcgcgg ggagacgtcc atgtagtcga cggctgccgg cgtgacgaag tcgacctcgc
     2221 cgcccttacg acggacgagc acgcggtctt cggcgaagtg gccgtcgtcc ttcaacggcg
     2281 cgtttgcctg cgcgatgacg aaacggtctt cctcatcggc ggtcaggtaa tcgacgtcgt
     2341 cggtgacctt gcccttgacg gcacgacggt acggcgtctc gacgaacccg aaggcgttga
     2401 tgcggccgtg cgttgcgagc gatccgatca ggccgatgtt cgggccctcg ggagtctcga
     2461 tggggcacat gcggccgtag tgactcgggt ggacgtcgcg gacttcgaaa ccggcacgct
     2521 cgcgtgacaa accgccgggg ccaagcgccg acagccggcg cttgtgggtc aagccggcca
     2581 acgggttggt ctggtccatg aactggctga gctgggaggt tccgaagaac tccttgatgc
     2641 tcgccacgac cgggcgaatg ttgatcaggg tctgcggcgt gatcgcctcg acgtcctggg
     2701 tcgtcatccg ctc
//
LOCUS       DASWVF010000332         9467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_9067, whole genome shotgun sequence.
ACCESSION   DASWVF010000332 DASWVF010000000
VERSION     DASWVF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9067"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VGD55_05900"
     CDS             complement(<1..777)
                     /locus_tag="VGD55_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY0869908.1"
                     /translation="MTISPGTFSFVSDLIRQQAAIVLTPGKEYLVESRLLPLARGAGL
                     DDVGALVAQLQRKPDDLLRRQIVEAMTTNETSFFRDRDPFNALTQVILPELCKVRSAT
                     RRINIWSAACSTGQEPYSIAMSVVDHPLITADWRVDVLATDINNEILERARRARYSQL
                     EVNRGLPAAMLVRHFERADTDWRVVAAVRKLVRFTPLNLADQFDLPQMDVVFLRNVLI
                     YFDPAMKSEVLRRVRRILRPDGFLFLGGAETTLGLDDGFDR"
     gene            complement(774..1928)
                     /locus_tag="VGD55_05905"
     CDS             complement(774..1928)
                     /locus_tag="VGD55_05905"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY0869909.1"
                     /translation="MADIGVLVVDDSVVIRRMVSSVLDADPDIEVVGTAANGRIALDK
                     LEQLHPDIIILDVEMPVMDGLATLRALRLTHPNLPVVMFSTLTERGAMATLDALAAGA
                     TDYVTKPSHAGSVAASMERVRLDLVPKVKGLVGAARARATFAARPAPTMPALPTGRPV
                     RQDRVDVIVIGSSTGGPDAVTSIASKLPADLAVPVLVVQHMPALFTRLFAGRLDRSCP
                     LNVVEAVDGQLVKAGTIIVAPGDHHLSVRRQGADVRVQLTQDPPENYCRPSVDVLFRS
                     AAAVYGSGVLGCVLTGMGRDGTKGAERIRAAGGRIVVQDAASSVVWGMPGAVVAAGHA
                     NDIVPLDRLAETLLAGAASGRRVGVPRLLPASAPRAAGFGGAADQSVRTR"
     gene            complement(2024..2932)
                     /locus_tag="VGD55_05910"
     CDS             complement(2024..2932)
                     /locus_tag="VGD55_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869910.1"
                     /translation="MYSLVNASTVGFDLARLPTGAAVATVLLEALAVQSPLGSRGHER
                     PSLHGFSTFDPLQAADPRRAAAWLEVSALAPPRRLDDALSVVGQLLDDVASAADPSGL
                     ASRQLEPVLRDLTTASFGGLEDLLRLVRLDILDESPEHVVSLASDALAAAYGGQRLSE
                     EVRTLFTVPWVSATRGLAPIPSDLGPYPEELRTILDRIATLTEGEVKVLVDGAAAVEA
                     DWSARMHAAAWAGYLSGRLRPAATAQFQAVRALRACGVDAASAARGVWNSISGCLQAV
                     VVHDILDEVTYGVLVAPWETAVGILG"
     gene            complement(3063..3497)
                     /locus_tag="VGD55_05915"
     CDS             complement(3063..3497)
                     /locus_tag="VGD55_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0869911.1"
                     /translation="MELRQYATFTVDSLLFGVEIHKVQEVIRHQDMTAVPLAGHGAAG
                     LINLRGEVVTAVDLRTRLGLPPRPDGMLPMNVVVRVDDEPVSLLVDTIGDVAHVSSDQ
                     FEEPPETTTGPGRVLVTGAYKLENALLLVLDVARAADIGLAV"
     gene            complement(3499..6249)
                     /locus_tag="VGD55_05920"
     CDS             complement(3499..6249)
                     /locus_tag="VGD55_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY0869912.1"
                     /translation="MDEFDEIVREFLVESYENLDQLDRDLVALEQEPDAHPLLQSIFR
                     TIHTIKGTSGFLAFGKLEVLTHAGEGLLSRLRDGGLRLTRERTTGLLQLVDAVRAILA
                     SIEATGHEDDRDYADLVAQLTALQTPDPEVEASIVPAQGRAIRPAAKRVGRPQAVLPT
                     PPAAVPPAAAESSAPAAPSSKRAPRPERRAVARPTRPSQDAQPVRPGDSPRTDVPSTD
                     LSGDSAPRVPKHDVKAARAARKAEAAGVLPAEQSSTTTGGAAGASASAGLPPNVPMLG
                     ELLQQQAEIDAEDVAVAMLEQQLGDTRPLGEILVDHGQATPEQVREALALQAVAKAEA
                     RAASGGSVTESTLRVDVVLLDTLMRLVGELVLTRNQIVASAATLADPGMQRSSQRLNL
                     LVSELQAGVMKTRMQPIDTVWNKLPRLVRDVAVACGREVGVVMEGRDTELDKTILEAI
                     KDPLTHVVRNAVDHGIEDPQVRVAAGKPAEGTLTLRAFHEGGQVNIEISDDGAGIDPA
                     RIALKAIERGVATVDQLRAMSPREITNLVFVPGFSTAAKVTNLSGRGVGMDVVRTNVE
                     KIGGTVDISSEPGVGTTLKIKIPLTLAIIPALTVECAGERYAVPQVSLDELLCLEGEQ
                     ARAGVEYLSGAPVFRLRGNLVPLVWLDHVLDLTKAPHVDDSVYILILQAEGRRFGLVV
                     DRVLNTEEIVVKPLSRQLKGIGTYAGATILGDGRVALILDVRALARRAQVVRESDSVE
                     SRDVRAVAQAVADGPEPLLLVAIGDRRLGVPLSMVTRLEEIAPDRIERVGQREVVQYR
                     GQLLPLGRLTTMLGEQAHADPTATLKVIVYTQGRRSVGLVVDAVVDIATEAPRSRGDV
                     EAYGVSGVAVVQELVTELLDMEQAIMATDPRFFDAVAAAGGADVSPYESYADDSFAVK
                     GT"
     gene            complement(6354..7223)
                     /locus_tag="VGD55_05925"
     CDS             complement(6354..7223)
                     /locus_tag="VGD55_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0869913.1"
                     /translation="MTIAPEQLGFTGMPRRLFVCTPSKLTTYLDCPRRYRMTYLDRPR
                     PPAGPPWAHNSFGASVHAALAGWWRLPRASRTADGAEGQLDAAWVSQGYRDPVQADGW
                     LARGRAMVRRYVATLDPDREPLGVERTLGARTPKLALAGRLDRLDDRDGQLVVVDYKT
                     GRRPLTVDDARGSLALALYAVAASATLRRPCVRVELHHIPTGAIHAFDHTGESLARQV
                     RRAEEIADEAVAATAALGAVAATTAVPASDAFPPHPGAQCGTCDFRAHCPEGAAAAPA
                     RLPWAALGEPGAS"
     gene            7361..8545
                     /locus_tag="VGD55_05930"
     CDS             7361..8545
                     /locus_tag="VGD55_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0869914.1"
                     /translation="MKLVHKAAATAVVGFVTVGTVSLIALGRAQDALHGMRAETVSAS
                     AVNAARGDVIFAMFCLVVGLLVALGALGLWLRHDVLKPLEVLRSRMASIADGDGDLTR
                     RLGFHRADEIGDLAGEFDRFVIGVQSLVGKVAGTATGLAASAANLSATSEQIATSAHD
                     ATRRAESAASAAEQVSANVASVAGATEEMGSSIREIAHNAHRAAGVAMDAVTMADTTN
                     AAISRLGESSSDIGSVVKLITEIAQQTNLLALNATIEAARAGEAGKGFAVVADEVKDL
                     AQETAKATDDISRRIESVRNDSRDAIAAIEAIRVVISQISDYQVTIASAVEEQSATTS
                     EMGRGYSEVATGSAEIARSIAGVASASSTTLNGVAAARLTTDELRKVADELKSLVSRF
                     TY"
     gene            complement(8555..>9467)
                     /locus_tag="VGD55_05935"
     CDS             complement(8555..>9467)
                     /locus_tag="VGD55_05935"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0869915.1"
                     /translation="RACCCRRLQFSWRPMALRSSLPRIKPVARERRAAGDSFTVDTAT
                     ATLVRDISRRAAWTLLLDGVESSHVDLDDPTVLEFEYVRWLGDIIDCLAPAGDPVDTV
                     HVGGGAMTLARYVMATRPASDQIVLELDGALVELVRERLPWARGRSSRRLRVRVGDAS
                     QAINGLADASWDVVVRDAFRDAEVPASLRTVENASRVKRVLRPTGVYLMNVTDRAPFA
                     VTGVELATLLEVFSDVAVISEPSVLRGRRHGNLIIAAANQPLPVEAIARRIADGGVQG
                     RVRDVGQATQLARGHRPFREADAGLLE"
BASE COUNT         1596 a         3177 c         3225 g         1469 t
ORIGIN      
        1 tcgatcgaag ccgtcgtcga ggccaagggt ggtctccgcg ccaccaagaa acaggaagcc
       61 gtcgggccgc agaattcgcc tcacccggcg caggacctcg gacttcattg ccggatcgaa
      121 gtagatcaag acgttgcgca ggaaaacgac gtccatctgc ggcaggtcga actgatccgc
      181 gagattgagc ggcgtgaagc ggaccagctt cctgacggca gccaccactc gccagtcagt
      241 atctgcccgc tcgaaatggc gcacgagcat ggcggctggc aggcctcggt tgacctccag
      301 ttgggagtac ctggcccgcc gggcccgctc aaggatctcg ttgttgatgt cggtagccag
      361 gacgtcaacc cgccagtcgg cagtgatgag cggatggtcg acgaccgaca tggcgatgct
      421 gtacggctcc tgcccggtcg agcacgcggc cgaccagatg ttgatgcgac gggtcgcgct
      481 gcgtaccttg cacagctcgg gcaggatcac ctgggtcagc gcattgaacg ggtcacggtc
      541 ccggaagaac gatgtctcgt tcgtcgtcat cgcttcgacg atctgacgcc ggagcagatc
      601 gtcaggcttg cgctgaagtt gcgcgacaag cgcgccgacg tcgtcgagcc cggccccgcg
      661 cgccagcggc aacaacctgc tctcgacgag gtactccttg ccaggggtca gcacgatcgc
      721 tgcctgttgc ctgatcaggt cgctgacaaa gctgaacgtg ccggggctga tcgtcacctg
      781 gtcctcacgg actggtctgc ggcgccgccg aaaccggcgg cgcgcggcgc cgacgcaggg
      841 agcagtcggg gcacgcccac ccggcgcccg cttgccgccc cggcgagcaa cgtttcggcg
      901 agccggtcga gcggcacgat gtcgttcgca tgccccgcgg cgaccaccgc gcctggcatt
      961 ccccacacca cggagctcgc ggcgtcctgc acgacgatcc ggccacccgc cgcccgaatc
     1021 cgttcggccc ccttcgtgcc gtcgcgcccc atgccggtga gcacacagcc gagcaccccg
     1081 ctgccgtaca cggcggccgc cgagcggaac aggacgtcga ccgaagggcg gcagtagttc
     1141 tcaggggggt cctgcgtcag ttgaacgcgg acgtcggctc cctggcgtcg gaccgagagg
     1201 tgatggtcgc cgggagcgac gatgatggtg ccggccttga cgagttgacc gtcaactgcc
     1261 tcgacgacgt tcagggggca actgcgatca agtcggcccg caaagagccg tgtgaacaga
     1321 gccggcatgt gttgcaccac aagaaccggc acagcaaggt ccgcgggcag cttcgatgcg
     1381 atcgaggtga ccgcgtctgg accgccggtc gacgagccga tcacgatcac gtcgacgcga
     1441 tcctgacgga ccgggcgtcc cgtcggcagg gcgggcatgg tcggcgccgg gcgcgccgcg
     1501 aacgtcgcgc gcgctcgcgc cgcaccgacg aggcccttga ccttcgggac cagatcgagt
     1561 cggactcgtt ccatcgaagc cgcgacgctg cctgcgtgcg acggcttcgt gacgtagtcg
     1621 gtcgcgccgg cggcgagagc atcgagggtc gccatcgcgc cacgttcggt gagcgtgctg
     1681 aacatgacga cgggcaggtt cgggtgcgtc aggcgcaatg cccgcaacgt ggcgagcccg
     1741 tccatgacgg gcatctcgac gtccaagatg atgatgtccg gatgcagctg ctcaagtttg
     1801 tcgagcgcga tgcgcccgtt cgctgcggta ccgacgactt cgatgtcggg atcggcgtcg
     1861 agaaccgacg acaccatgcg gcggatgaca accgaatcat cgacgacaag aacgccgatg
     1921 tcggccaccg cacacctccc gcagcgtgag tgccgctcgc agtgtgcggt ccggcactag
     1981 accatcggca cgagcggccc aggactgagg cgcggttact tcgtcagccg agaatcccca
     2041 ctgcggtctc ccacggcgcc accagcaccc cgtacgtcac ttcgtcgagg atgtcgtgca
     2101 ccacgaccgc ctgaaggcat ccgctgatgg agttccagac accgcgcgcc gccgacgccg
     2161 cgtcgacccc gcaggcgcgc aacgcacgaa cggcctggaa ttgagccgtc gccgcaggcc
     2221 gcaaccgccc cgatagatag cccgcccacg cggcggcgtg catgcgagcg ctccaatcgg
     2281 cctcgaccgc ggccgctcca tcgacgagga ccttcacctc cccctcggtc agcgtcgcga
     2341 tccgatcaag gatcgtgcgc agctcttcgg ggtaggggcc aagatctgac gggattggtg
     2401 cgaggcctcg ggtggcgctc acccagggca ccgtgaacag ggtgcggact tcttcgctca
     2461 ggcgctgccc gccgtacgcc gccgcaagag cgtcgcttgc gagcgagacg acgtgttcgg
     2521 ggctctcatc gaggatgtcg agcctgacca ggcgaagcag atcctccaag cctccgaacg
     2581 acgccgtggt caggtcgcgc agcaccggct caagctgtct gctggcgagc ccgctgggat
     2641 cagcggcgct ggcgacgtcg tcgagtagct gcccgactac cgagagcgcg tcgtcgaggc
     2701 gacgcggcgg cgccagggcg gaaacctcga gccaggccgc cgcccggcgc gggtcagccg
     2761 cctgcagtgg atcgaaggtg gagaacccgt gcaggctcgg ccgttcatgg cctctggatc
     2821 cgagcgggct ctgaacggcg agagcctcaa gcaggacggt cgcgactgcg gcgccggtgg
     2881 gcagtctcgc gaggtcgaaa ccgacggtcg atgcattgac gagcgaatac aacggaggcc
     2941 ctccggcggt tcgagggctg gaggcggcac gggtaaccgt gacctgccgg cagctcaggg
     3001 acctcgatcc tcccattgcc aaggccgctg gcgagacggc cgaccgggcg aactgcgccg
     3061 ggtcagaccg ccagaccgat atcggcggcc cgcgccacat cgagcaccag aaggagcgcg
     3121 ttctcgagct tgtaggcgcc cgtcacgagc actcggccag ggccggtggt ggtctcaggc
     3181 ggctcctcga actggtcgga ggagacgtgg gcgacatcgc cgatcgtgtc gacgagcagg
     3241 ctgaccggtt cgtcgtctac ccgtacgacg acgttcatcg gcagcatgcc gtcaggacgc
     3301 ggcggaaggc cgagccgggt gcgcaggtcg actgcggtaa cgacctcacc gcgcaggttg
     3361 atgaggccgg ccgcgccgtg gccggcaagg ggcaccgcgg tcatgtcctg gtgacgaatg
     3421 acttcttgca ccttgtggat ctcgacgccg aagagcagcg agtcgaccgt aaatgtggcg
     3481 tactggcgca gttccatgtc aggtcccctt cacggcgaac gagtcgtcgg cgtagctctc
     3541 gtacggcgac acgtcggcgc caccggcggc cgcgaccgca tcgaagaagc gggggtcggt
     3601 cgccatgatc gcctgctcca tatcgagcag ctcggtcacc agttcctgga cgacagccac
     3661 accggagacg ccatacgcct caacgtcgcc ccgcgagcgc ggtgcctcgg tcgcgatatc
     3721 gacgacggcg tcgacgacaa gtcctacgct tcggcggccc tgcgtgtaaa cgatgacctt
     3781 caacgtcgca gtgggatcgg cgtgcgcttg ctcgccgagc atcgtcgtca gacgaccaag
     3841 cggcaatagc tgcccgcggt actgcacgac ctcgcgctgg ccaacgcgct cgatgcgatc
     3901 gggtgcgatc tcttcgagac gggtcaccat cgacagcggg acgccgagac gccggtctcc
     3961 gatcgcgacg agaagcagtg gctccggacc gtcggcaact gcctgcgcaa ctgcgcgcac
     4021 atcgcggctc tcaacggagt cgctctctcg caccacttgg gcccggcggg ccagcgcacg
     4081 aacgtcgagg atcaatgcca cgcggccgtc gccgagaatc gtcgcgcccg cgtaggtacc
     4141 gatccctttc agttgacgcg acagcggctt gaccacgatc tcctctgtgt tgagcacccg
     4201 gtcgacgacg aggccgaacc ggcgtccctc ggcttgcaga atgaggatgt agacggagtc
     4261 gtcgacgtgc ggtgccttgg tgaggtcgag cacgtgatcg agccacacaa gtggcacaag
     4321 gttcccgcgc aatcggaaga ccggtgcgcc tgacaggtac tcgacaccag ccctggcctg
     4381 ctcaccttcg aggcacaaca gctcgtcgag gctgacctgg ggaaccgcgt accgctcgcc
     4441 cgcgcactcg acagtgagcg cagggatgat cgcgagcgtc agcgggatct tgatcttcag
     4501 ggtcgtgccg acaccgggtt cgctggagat gtcgacggtg ccgccgatct tctcgacgtt
     4561 ggtccgcacg acgtccatgc cgacaccgcg gcccgacaag ttggtcacct tggcggccgt
     4621 agagaagccg gggacgaaca cgaggttcgt gatctcacgc ggcgacatgg cgcgcagctg
     4681 atcgactgtt gccacgccac gctcgatggc cttcagagcg atcctggccg gatcgatacc
     4741 cgcgccgtcg tcgctgatct cgatgttgac ctgtccgcct tcgtggaacg cgcgcaatgt
     4801 gagcgtgccc tcggccggct tgccggcggc gacacgcacc tgcgggtctt cgatcccgtg
     4861 gtcgacggca ttgcggacca catgcgtgag cggatccttg atcgcctcaa ggattgtctt
     4921 gtcgagctcg gtgtcacgcc cttccatgac caccccgacc tcgcggccac atgccactgc
     4981 gacgtcgcgc acgaggcgcg ggagcttgtt ccagacggtg tcgatcggct gcatccgagt
     5041 cttcatcacg ccggcctgca gctcgctcac aagcaagttc aggcgttgtg acgagcgctg
     5101 catgccgggg tcggccaacg tcgccgcgct cgcgacgatc tggttgcggg tcaacacgag
     5161 ttcgccgacg aggcgcataa gggtgtcgag caagacgacg tcgacccgca gcgtcgactc
     5221 ggtgactgat ccaccactcg ccgcgcgagc ttctgccttg gcgacggctt gcagggcgag
     5281 tgcctcgcgc acttgttcag gcgttgcctg cccgtggtcg acgaggatct caccgagcgg
     5341 ccgcgtgtcg ccgagttgct gctcgagcat ggcgacggcg acgtcttcgg cgtcgatctc
     5401 ggcctgctgc tgaagcagct cgccgagcat gggcacgttg ggtggcaggc ctgctgacgc
     5461 agacgccccg gccgccccgc cggtcgtggt cgacgattgc tcggctggga gcacccccgc
     5521 tgcctccgct ttgcgggccg cgcgggcagc tttcacatcg tgcttgggga cccgtggcgc
     5581 ggaatcaccc gagaggtccg tcgaaggaac gtcagtgcgc ggggagtctc cgggccgaac
     5641 gggttgggcg tcttgagacg gccgagtggg tcgggcgacc gcccggcgtt ccggtcgggg
     5701 tgcgcgcttg gaacttggcg cggcaggagc gctggactcg gcagccgccg ggggcacggc
     5761 tgccggaggt gtcgggagta ctgcctgagg ccgtccgacc cgctttgctg ccggcctgat
     5821 ggcgcggccc tgtgccggca cgatgctcgc ttcgacctcg gggtcgggtg tctggagagc
     5881 ggtcagctgg gctaccaggt cggcgtagtc acggtcgtct tcgtggccgg tcgcctcgat
     5941 cgaagccaga atcgctcgaa ccgcgtcgac gagttgcaac aagccggtgg tgcgctcgcg
     6001 cgtcagccgg agtccgccgt cgcgcaggcg ggacaacaac ccttcgccgg catgggtgag
     6061 cacttcgagc ttgccaaagg cgaggaaccc gctcgtccct ttgatcgtgt ggatcgtgcg
     6121 gaagatgctt tgcagcaacg gatgggcgtc gggctcctgc tcgagcgcga caagatcacg
     6181 gtcgagctga tcgaggttct cgtagctctc cacgaggaac tcgcgcacga tctcgtcaaa
     6241 ctcgtccatc aacactcctc cgcgccctgc ccggacgcac cgagccaccc aacggtcggt
     6301 cacgacgatc actcatcggc gcatctggcc gacaactgag ccctagatcg cgtctagctc
     6361 gcgcctggct cgccgagcgc ggcccacggc agccgcgccg gtgcggccgc ggcgccttcg
     6421 gggcagtggg ctcgaaagtc gcacgtgcca cattgcgcgc cagggtgtgg cggaaaggca
     6481 tcagaagcag ggacggcggt cgttgctgcc accgccccga gggcagcggt cgcggcaact
     6541 gcttcgtcgg cgatctcctc ggcgcggcgc acctgcctgg ccagcgactc accggtgtga
     6601 tcgaacgcgt ggatggcgcc ggtcgggatg tggtgtagct caacccgcac gcacggacgc
     6661 cgaagtgtcg ccgaggccgc cacggcgtac agcgccaggg ccagcgagcc gcgcgcgtca
     6721 tcgacggtca ggggtcggcg gccagtcttg taatcgacca cgaccagctg gccatcgcgg
     6781 tcatcgagcc ggtcaagccg gccagctagg gcgagcttgg gggtgcgggc gcccagagtg
     6841 cgctcgacac cgagcggctc tcgatcggga tcgagcgtgg cgacatagcg ccgcaccatg
     6901 gcgcgaccgc gcgccagcca cccgtcggct tgcacggggt cgcggtaccc ctgcgacacc
     6961 caggctgcat cgagttggcc ctcagcgccg tcagccgtac ggctggcgcg gggcagtcgc
     7021 caccagccgg caagcgccgc gtggacgctc gcgccgaagc tgttgtgggc ccacggtgga
     7081 ccggccggcg gccgagggcg gtcgaggtag gtcatgcggt agcgccgcgg gcagtcgagg
     7141 taggtcgtga gcttcgaagg agtgcacacg aacagccggc gcggcatgcc ggtgaatcca
     7201 agttgctcag gcgcgatcgt cacgaacccc atggtggcct ctggtgggga cagataccgg
     7261 ctgtgacggg gtcctgagct agctcagctt ggtggcacct cctgccgaag gagattctca
     7321 ggcgccccac cgtgcgggtg cgcgtgatct ggaggcaccg atgaagctcg tccacaaggc
     7381 cgccgcgacc gccgtcgtcg gcttcgtgac agtgggcaca gtcagcctga tcgcgcttgg
     7441 gcgggcccag gacgcattgc acggcatgcg ggcggagacg gtcagcgcga gcgccgtcaa
     7501 cgctgcgcga ggcgacgtga tcttcgccat gttctgcctc gtcgtcgggc tgttggtcgc
     7561 cctgggtgca cttggcctct ggcttcgaca cgacgttctc aagccactgg aggtgctgcg
     7621 cagccggatg gccagcatcg ccgacggcga cggcgacctc acgcgccgac tcggatttca
     7681 ccgcgccgac gagatcggcg acctcgctgg cgagttcgat cgcttcgtca tcggggtgca
     7741 gtcgctggtc ggcaaggtcg cgggcaccgc gactggattg gccgcgtcag ccgcgaacct
     7801 ttccgcgacc tcggagcaga tcgcaacgtc cgcgcacgac gcgacccgcc gggccgagag
     7861 tgctgctagc gcggcagagc aggtgagcgc caacgtggca agcgtcgccg gagcaactga
     7921 agagatgggc tcgtcgatcc gcgaaatcgc gcacaacgcc catcgcgcgg ccggtgtcgc
     7981 gatggacgcc gtgacgatgg ccgacacgac caacgcagcg atctcacgac tgggtgagtc
     8041 gtcgtccgac atcggcagcg tcgtgaagct catcaccgag attgcccagc agacgaacct
     8101 cctcgcgttg aacgcgacca tcgaggccgc ccgcgctggc gaggccggca agggatttgc
     8161 ggtcgtcgcc gacgaggtca aggacctcgc gcaagaaaca gcgaaggcga ccgacgacat
     8221 ctcccgccgc atcgagtcgg tgcgcaacga ttcgcgcgac gcgatcgcgg ccatcgaggc
     8281 gatccgtgtc gtcatttcgc agatcagcga ctaccaggtg acgattgcct cagctgtcga
     8341 ggaacagtcg gcgacgacca gcgagatggg ccgcggctac agcgaagtcg ccaccggcag
     8401 cgcggagatc gcgcggtcga tcgcgggcgt cgcgagtgcg tcgtcgacca cactgaacgg
     8461 agtcgcggcg gcgcggctca ccactgacga attgcgcaag gtagccgacg agttgaagtc
     8521 gctcgtctca cgcttcacct actgagccgc gtacctactc caaaaggccc gcgtcggctt
     8581 cgcggaacgg gcgatggccc cgcgcgagct gcgttgcctg gccgacatca cgcaccctgc
     8641 cttgcactcc gccgtccgcg atgcgccgtg caatagcttc gacgggtagc ggttggttgg
     8701 cggcggcgat gatgaggttg ccgtggcgtc ggccacgcaa gacgctcggt tccgaaatca
     8761 ctgcgacgtc gctgaagact tcgagcagcg tcgccagttc gacgcccgtg acagcgaacg
     8821 gcgcccggtc ggtgacgttc atgaggtaga cgccggtagg gcgcagaact ctcttcaccc
     8881 gactcgcgtt ttcaaccgtg cgaagtgacg ccggcacctc ggcgtcccga aatgcatctc
     8941 gaacgacgac atcccacgac gcatcagcga gtccattgat cgcctggctc gcatcgccaa
     9001 cccgcacccg cagcctccga ctgctgcgac ctcgtgccca cggcagccgc tcgcggacga
     9061 gctcgaccag cgctccgtcg agttcgagca caatctgatc ggacgccggg cgggtggcca
     9121 tcacgtagcg cgcgagggtc atggcacctc ccccgacgtg cacggtgtcg accggatctc
     9181 cggcgggtgc gaggcaatcg atgatgtcgc cgagccaccg cacgtattcg aattcgagca
     9241 ccgtcgggtc atcgagatcg acgtgcgaac tctctacccc gtcgagcaac agggtccagg
     9301 cagcgcgccg cgagatgtcg cgcacgagcg ttgcagtcgc ggtgtcaacc gtgaaactgt
     9361 caccggcggc ccgccgttcg cgggccaccg gcttaatgcg cggcaacgaa cttcgcaatg
     9421 ccatcggccg ccaactgaac tgcaatcgac gacagcagca ggcccgc
//
LOCUS       DASWVF010000333         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_442335, whole genome shotgun sequence.
ACCESSION   DASWVF010000333 DASWVF010000000
VERSION     DASWVF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442335"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VGD55_05940"
     CDS             complement(<1..1339)
                     /locus_tag="VGD55_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869916.1"
                     /translation="MATSAAIALAASIGVVGTAQADSAITPASFTATVAPQLVDAGVS
                     QKSFALTITNCATTVTNCTGPSGQNLGSATIQLPAGFANVSASVSAAGWSIVTPIVGG
                     LVQVDANQNGDDDAPSPNQLTPGQSIVVTIVADTPSTSGYYTFTTAAENDPVGDGPEE
                     DDPADEPFPFAGPQPQVGVGIPDHLVFVGQPSDVQVTSGASTPFICPPPSLEVVTADG
                     SPVTSGSASVSLLADTAFGNPGLGGTTTATTVGALATFGTAACAGGVDASTVGSNFKL
                     KATATWTLGGLTIPLVTAADSSPFSVVQLITTCAAGQTCNGAATDTHTSVKVATTSAT
                     TTDQLEIAIGADSFAGTTCHPFFQPNGIEVTRVVANDRGKTVTLTFDKTIVNQKPLYL
                     ILLFPICFSAPWGNWVTDLGRAPTFNTTTNEFEGLLPICLPRFLPAGNPCVQSR"
     gene            complement(1525..1842)
                     /locus_tag="VGD55_05945"
     CDS             complement(1525..1842)
                     /locus_tag="VGD55_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869917.1"
                     /translation="MSWIVGLPAASAVVVLEAGALEAAARDGAAWEDPAWDDAAGDAE
                     AVADADAGELAEFEADGETAGLGELPPQATSNIAVRATVNTVRLQTKRSEDIATSKAS
                     SKR"
BASE COUNT          321 a          596 c          651 g          342 t
ORIGIN      
        1 tacgcgactg cacgcacggg ttgccagccg gcaagaaccg tggcaagcag atcggcagca
       61 gaccttcgaa ctcgttggtc gtggtgttga aggtcggagc acgaccgagg tcggtgaccc
      121 agttccccca cggcgccgag aagcagatcg ggaacaacag gatcaggtac agcggcttct
      181 gattgacgat ggtcttgtcg aatgtgagag tcaccgtctt gccgcggtcg ttggcgacca
      241 cgcgggtgac ctcgatgccg ttcggctgga agaacgggtg gcaggtcgtg ccggcgaacg
      301 agtcggcacc gatcgcgatc tcgagctgat ccgtggtggt cgcgctggtc gtcgccacct
      361 tgaccgaagt gtgggtgtca gtggcggcac cgttgcacgt ctgacctgcg gcgcaggtgg
      421 tgatgagctg aacgacgctg aacgggctgg agtctgcggc ggtcacgagc ggaatcgtga
      481 ggccgccgag ggtccaggtt gccgttgctt tgagcttgaa gttcgacccg acggtcgaag
      541 catcgacgcc tcccgcgcac gcggcagtac cgaaggtggc gagcgcaccg acggtcgtcg
      601 ccgtcgtcgt gccaccaaga cccgggttac cgaacgcagt gtcggccagc aggctcaccg
      661 aggcgctgcc cgacgtgaca ggagagccgt cagcggtgac gacctcgaga ctcggcggtg
      721 ggcagatgaa aggagtcgag gcaccgctgg tgacctgcac gtcactgggc tgcccgacga
      781 agacgaggtg gtcggggatg cctaccccga cctgcggctg cgggccggcg aacgggaacg
      841 gctcgtcagc agggtcgtcc tcttcggggc cgtcgccgac cgggtcgttc tcggcggcgg
      901 tcgtgaaggt gtaataaccg ctggtgctcg gagtgtcggc gacgattgtg acgacgatcg
      961 actgcccagg tgtgagctga ttcggcgacg gcgcgtcgtc gtcgccattc tggttggcgt
     1021 cgacctgcac gagcccgccg acgatcggcg tgacgatcga ccagccggcc gccgatacgc
     1081 tggccgacac gttcgcgaac ccggctggca gctgaatggt ggcggagccg aggttctggc
     1141 cgctcggtcc ggtgcagttg gtcaccgtcg tcgcacagtt ggtgatggtc agggcgaagg
     1201 acttctgcga tacgccagca tcgactagct gcggcgcgac ggtcgcggtg aaggaggccg
     1261 gagtgatggc gctgtcagcc tgggccgttc cgacgactcc gattgaagcg gcgagcgcga
     1321 tcgcggccga cgtcgccatt gcgaacgcct tccgcgtcgc cgatccacga acacgtgacg
     1381 acatttcgta gtcccctccc catcaagcaa tccggcgcat gcgaacatgc cactgcgtcg
     1441 tgcaggcgca gccccggtag cccccatctc gcttagcagt gtttgtatcg cggccggtgc
     1501 ctatcgagcc atcatccgaa atgactacct cttggaggag gctttacttg tggctatgtc
     1561 ctccgaccgc tttgtctgca accgcaccgt attcaccgtt gctctgacag cgatgttgct
     1621 tgttgcctgc ggtggcaact cacccaagcc agcggtctcg ccgtcggcct cgaactcggc
     1681 cagttctccg gcgtctgcat ctgcgacggc ttcggcatcg ccagccgcat cgtcccaagc
     1741 tggatcctcc caagctgcac cgtcgcgagc tgcggcttcg agtgcgccgg cttcgagcac
     1801 gacgacggcg gacgccgcgg gcaacccgac tatccagctc atgcggtctc accagatcgt
     1861 caaaggcggc gtcggaaccg tcgacatcga cgacatgtcg gtcgtcggat
//
LOCUS       DASWVF010000334         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_264028, whole genome shotgun sequence.
ACCESSION   DASWVF010000334 DASWVF010000000
VERSION     DASWVF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264028"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VGD55_05950"
     CDS             <1..233
                     /locus_tag="VGD55_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869918.1"
                     /translation="ELHGPRPVSGVASGQRVAAVITSLAPAGLADEPLPAGPILLVDD
                     VYSSGWTMTVAASLLGELGASTVYPLVLHRRP"
     gene            279..485
                     /locus_tag="VGD55_05955"
     CDS             279..485
                     /locus_tag="VGD55_05955"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEY0869919.1"
                     /translation="MARISALVAEEHHLTGGAADTAARRRELEEQLDQCWDLLRQRQA
                     KREFGEDPSQAHARPISEVEHYQQ"
     gene            complement(529..>1658)
                     /locus_tag="VGD55_05960"
     CDS             complement(529..>1658)
                     /locus_tag="VGD55_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HEY0869920.1"
                     /translation="TDGQPDGSAAPLTWGAAAQVRLTADLAAGRVVEKPPSTTARYVT
                     HTQAATTLTVTSPANNTAATGTIPVIGTGAPHAKVVIADVATDNNDATTLFSATAAMS
                     GSFNIPVTVAAGTNVLVVTAMTASGATAQTTVTLVNDVVNGTLIFSASDPDNDDNGPG
                     NYAYPTDSAFHPGAFDLEQFQVYDTGTTVTFRVQTRDLTPTFGPDNGAQLIDLYVHNP
                     AAATTSTAAAFASRNYTIAGADAWSRLIEVQGFGQLFVDPSNASQGPVTIRTNPISRY
                     ITFSVDKTALGGTPTSGWAFTIALTGQDGFGQDNARTFAPTPQPFNFGVCAAASADPH
                     CTADPNTVPKVMDTITPAGVLQSTELDYTLGAVVLQGVTIP"
BASE COUNT          241 a          534 c          576 g          307 t
ORIGIN      
        1 tggaactgca cggtccccgg ccagtgtctg gcgtcgcctc gggccagcgg gttgcggctg
       61 tgatcacgag tcttgcgccc gcgggccttg ccgacgaacc gcttcctgcg ggcccgatcc
      121 tgcttgtcga cgatgtctac agctccggct ggaccatgac tgtcgcggcg tccctgctcg
      181 gcgaactcgg ggccagcacg gtctacccgc tggtcctgca tcgccgcccg taggcttgtc
      241 ataacccctg gaggaaaaac ctggccgacg acgacctgct ggcgcggatc tccgcgctcg
      301 tggccgaaga acatcacctc accggcgggg cggccgacac tgccgcccgg cgacgcgaac
      361 tcgaggagca gctcgaccag tgctgggacc tgcttcggca gcgccaggcc aagcgcgaat
      421 tcggcgaaga cccaagccag gcgcacgctc ggccgatctc cgaggtcgag cactaccagc
      481 agtaaccgct ttccgatcgg cggcgcgact tggcccgctc cctggctgct acggaatcgt
      541 gactccctgg agcactaccg ccccgagcgt gtagtcgagt tcggtcgact gcagcacgcc
      601 tgctggcgtg atggtgtcca tcaccttcgg cacggtgttc gggtcggcag tgcaatgcgg
      661 gtcggccgac gcggctgcgc acacgccgaa gttgaacggc tgcggcgtgg gcgcgaacgt
      721 ccgcgcattg tcttggccga atccgtcttg gccagtgagc gcgatggtga acgcccatcc
      781 cgatgtcggc gttccgccca gcgcggtctt gtcgacgctg aacgtgatgt agcgcgagat
      841 cgggttggtg cggattgtca ccgggccttg gctggcattg ctcgggtcga caaacagttg
      901 cccaaagccc tgcacctcga tgagtctcga ccacgcgtcg gcgcccgcaa tcgtgtagtt
      961 ccgtgatgcg aacgcggcgg cggtcgacgt cgtggccgcc gctgggttgt ggacgtacag
     1021 gtcaatcagc tgcgcgccgt tgtcagggcc gaacgtcggg gtcaggtcgc gcgtctgcac
     1081 gcggaaggtg accgtcgtcc cggtgtcgta gacctggaac tgctcgagat cgaacgcgcc
     1141 ggggtggaaa gcactgtccg tcgggtaggc atagttgccc ggaccgttgt cgtcgttgtc
     1201 cgggtcgctc gcgctgaaga tcaacgtgcc gttcacgacg tcgttgacga gcgtgacggt
     1261 ggtctgggca gttgcaccgg aggccgtcat ggccgtgacg accagcacat tcgtgccggc
     1321 cgcgacagtc accggaatgt tgaagctgcc gctcattgcc gccgtcgcgc tgaacaacgt
     1381 cgtcgcatcg ttgttgtcgg tggcgacgtc ggcgatcacg accttggcgt gcggcgcccc
     1441 agttccgatc actgggatag tgccggtggc tgcggtgttg ttggcgggtg acgtgaccgt
     1501 cagcgtcgtg gcagcttgcg tgtgggttac gtatcgggcg gtggtcgacg gtggcttctc
     1561 cacgacgcgg ccggcggcga ggtcagcggt cagccgcacc tgcgccgccg caccccacgt
     1621 cagcggtgct gccgaaccgt cgggttgccc atcggtgt
//
LOCUS       DASWVF010000335         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_365586, whole genome shotgun sequence.
ACCESSION   DASWVF010000335 DASWVF010000000
VERSION     DASWVF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365586"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGD55_05965"
     CDS             complement(<1..294)
                     /locus_tag="VGD55_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY0869921.1"
                     /translation="MLIHPWDAAIDDAEWRDWIADGHDFGQLIAAGGPERQWPNVVPT
                     HFVLDGAVVVLHLARPNPVWHAIEENRRVVMSVTGDYTFIPGYWRTAEGLPSEY"
     gene            complement(321..1913)
                     /gene="serA"
                     /locus_tag="VGD55_05970"
     CDS             complement(321..1913)
                     /gene="serA"
                     /locus_tag="VGD55_05970"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0869922.1"
                     /translation="MSKPIVLIAEELSPAGIDLLSGDFEIREVDGADRAALLPALATA
                     DAVIIRSATKIDAEAIAAAPNLKVVARAGVGLDNVDVKAATNAGVMVVNAPQSNIVSA
                     AEQAIALLFAVARRTPIANASLKSGEWKRSKFTGVEILDKTVGVVGLGKIGQLVAQRL
                     AAFGTHLIAYDPYVQPARAAQYGVRLVTLEELLRESDIITIHLPKTPETIDLISEREL
                     GMVKPGVIIVNAARGGLINEDALAIAVKDGRVGGVGLDVFATEPTTSSPLFEFDNVVV
                     TPHLGASTDEAQEKAGTAVARSVKLALSGEFVPDAVNVQGGAIAEDVRPGLPLAEKLG
                     RVFTALAGGIATRIEVEVRGEIAAYDVKVLELAVLKGVFTDVVEKSVTYVNAPLFAQD
                     RGVEVSLTTDDESPDFRNLITVRGTLPNGHQLSVGGTLAGTKHVEKLVKVDDFDVDLS
                     LTEHLGFFRYVDRPGVVGVLGRILGEHGINIAGMQVARDTRGGHALGALTVDSALPAA
                     VLETIVSEIGAESGRAVDLVEV"
     gene            2133..2372
                     /locus_tag="VGD55_05975"
     CDS             2133..2372
                     /locus_tag="VGD55_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869923.1"
                     /translation="MGSNRVRTLMVLETLMFFNGLLCAGVGWILLFFMAKPAGLIGAL
                     IFWVAATVLWEGKRYVDRLLPFDGSHRGRSRVRRS"
     gene            complement(2536..3537)
                     /gene="ilvC"
                     /locus_tag="VGD55_05980"
     CDS             complement(2536..3537)
                     /gene="ilvC"
                     /locus_tag="VGD55_05980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY0869924.1"
                     /translation="MPVEIFYDDDTDLSIIQNRHVAVLGYGSQGHAHALSLRDSGVDV
                     RVGLLEGSKSRGKAEAEGLRVLTPDAAVEEADLIMVLAPDPVQRHLYKEAIEPNLVDG
                     DALFFGHGLNIRYGLITPPSGIDVCMVAPKGPGHLVRRQFEEGRGVPVLLAVEQDATG
                     KAWDLVKSYAKGIGGTRAGALKTTFKEETETDLFGEQVVLCGGLTELIKAGFETLVGA
                     GYQPEVAYFECLHEVKLIVDLMYEGGISKMYWSVSETAEYGGYTRGPRIVTDATKAEM
                     QKILREIQDGEFAREWMAEYDSGLPNYKRYYAETVDHPIEVTGRALRPMMSWITDAE"
     gene            complement(3604..>3796)
                     /locus_tag="VGD55_05985"
     CDS             complement(3604..>3796)
                     /locus_tag="VGD55_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0869925.1"
                     /translation="KVIDVAADAVTIEATGGAEKIDAFLRVLEPFGVKELVQSGMVAM
                     GRGSRSITDRTLRPVDRIA"
BASE COUNT          633 a         1264 c         1235 g          664 t
ORIGIN      
        1 gtactcggac ggcaacccct ccgccgtccg ccaatagccc ggtatgaacg tgtagtcacc
       61 ggtgacgctc atcacgacgc gacggttctc ctcgatcgca tgccacaccg ggttcgggcg
      121 agccagatgc agcaccacaa ccgcgccgtc cagaacgaaa tgagtcggga cgacgttggg
      181 ccactgccgc tcgggaccgc cggccgcgat gagctgaccg aagtcgtggc cgtcggcaat
      241 ccagtcgcgc cactcggcgt cgtcgatcgc cgcgtcccac ggatgtatca gcacgtcgcg
      301 atcacctccg ggcgcctcgc tcagacctcg acgagatcaa ccgcacggcc cgactcagca
      361 ccgatctcgc tgacgatcgt ctcaagcacc gccgcgggca acgcggagtc gacagtcagc
      421 gccccgagcg catgcccgcc acgcgtatca cgggcgacct gcatgccggc gatgttgatg
      481 ccgtgctcac cgaggatccg accgagcacg ccgacgacac ccgggcggtc gacgtaacgg
      541 aagaatccca ggtgctcggt gagcgagagg tcgacgtcga aatcatcgac tttcacgagc
      601 ttctcgacgt gcttggtgcc cgccaaagtg ccgccgaccg agagctggtg cccgttcggc
      661 aaggtgccac gcacggtgat gagattgcgg aagtcggggc tctcgtcgtc agtggtgagg
      721 ctcacctcga cgccacggtc ttgcgcgaac agcggcgcgt tcacgtaggt cacggacttc
      781 tcgacgacgt cggtgaacac acccttcaag accgcgagct caagcacctt cacgtcgtac
      841 gccgcgatct cgccgcgtac ctcgacctcg atgcgggtcg cgatgccgcc ggcaagtgcg
      901 gtgaagacgc gaccgagctt ctcggccagc ggcaagccgg ggcgtacatc ttcggcaatc
      961 gcgccgccct gcacgttgac ggcgtcaggc acgaactcac ctgacaatgc gagcttgacc
     1021 gagcgcgcga ctgcagtgcc ggccttctcc tgtgcctcgt cagtgctcgc gccgaggtgc
     1081 ggcgtcacga ccacgttgtc gaattcgaac agcgggctgg acgtcgtggg ctcggtggcg
     1141 aagacgtcga ggccgacacc gcccacccga ccgtccttca ccgcgatggc gagggcatct
     1201 tcgttgataa gcccgccacg agcggcgttg acgatgatga cgcccggctt caccatgccg
     1261 agctcgcgct cgctgatgag gtcgatggtt tctggcgtct tgggcaggtg gatcgtgatg
     1321 atgtcgctct cgcgaagcag ctcttcgaga gtgacgagcc gcacgccgta ctgcgctgcg
     1381 cgcgccggct gtacgtaggg gtcgtacgcg atcagatgcg tgccgaacgc ggcgaggcgc
     1441 tgggcaacga gctggccgat cttgccaaga cccacgacgc cgacggtctt atcgaggatc
     1501 tcaacgccgg tgaacttcga ccgcttccac tcacctgact tcagcgaggc gttcgcaata
     1561 ggtgtgcggc gggcgacggc gaacagaagc gcgatcgcct gctcggcggc actgacgatg
     1621 ttcgactgcg gcgcgttcac gaccatcacg ccggcgttcg tcgctgcctt cacgtcgacg
     1681 ttgtcgagac cgacgcctgc acgcgcgacg accttcaggt tcggcgccgc ggcgatcgcc
     1741 tcggcgtcga tcttcgtggc gctgcggatg atgacggcat cggccgtggc gagcgccggc
     1801 agcagcgctg cgcggtcggc gccgtcgacc tcacgaatct cgaaatcgcc gctcagcagg
     1861 tcgataccag ctggggagag ttcttctgcg atgagaacaa tgggcttgct catagctggg
     1921 caatcctccg acgtaagacc cgaatcggga tgttggggtc gtcggcgttg gccaccacgc
     1981 tgtagcacgc gccttgaggc cccgattcta gcgcgcaggt ttcaggcacg tttcacgcac
     2041 ccggcgcccc ggtggggctg gcactgcggg cggccagagc gcacgatgtg tcaaggcgct
     2101 gtcatgacgc tcaggcacgc ggaggaggtc cggtgggctc gaatcgcgtg cgcacgctga
     2161 tggtgctgga gacgctgatg ttcttcaacg gcctcctgtg cgcaggcgtc gggtggatct
     2221 tgctcttctt catggcgaag ccggccggcc tgatcggcgc tctgatcttc tgggtcgcgg
     2281 cgaccgtgct gtgggaaggc aagaggtacg tcgaccgact gctgcctttc gacggctcgc
     2341 accgcggtcg cagtcgagtg cgccgcagct gatcgccgtc cgccctcaac ggcggcgcgg
     2401 ctgaccggcg tccgccctcg ttcttgatca tcgcgaagcc ctggacaggc tgcggtcccc
     2461 gaagacaggc gtgggagccg gtcctcgggg gtcggaaccc cctgaacgtg atcgatgcgc
     2521 gtcgaacggg ccggcctact cggcgtcggt gatccagctc atcatcgggc gcagggcgcg
     2581 accggtcacc tcgatcgggt ggtcgacggt ctcggcgtag tagcgcttgt agttcggcag
     2641 ccccgagtcg tactcggcca tccactcgcg agcgaactcg ccgtcttgga tctcgcggag
     2701 gatcttctgc atctcggcct tcgtggcgtc ggtcacgatg cgcggacccc gggtgtaccc
     2761 gccgtactca gcggtctcgg acaccgacca gtacatcttc gagatgccgc cctcgtacat
     2821 gaggtcgacg atcagcttca cctcgtgcag gcactcgaag taggcaacct cgggctggta
     2881 gccggcgccg acgagcgtct cgaagccggc cttgatgagc tcggtcagcc caccgcacag
     2941 cacgacctgc tcgccgaaga gatcagtctc ggtctcctcc ttgaacgtcg tcttcagcgc
     3001 gccggcccgc gtgccaccga tgcccttcgc gtaggacttc acgaggtccc acgccttgcc
     3061 ggtcgcgtcc tgctcgaccg cgagcaggac cggcacgccg cgaccttctt cgaactggcg
     3121 tcgcaccaga tgtccaggtc ctttcggcgc caccatgcag acatcgatac cggacggcgg
     3181 ggtgatgagg ccgtagcgga tgttcagacc gtgcccgaag aaaagcgcgt cgccgtcgac
     3241 caggttgggc tcgatggcct ccttgtagag atggcgctgc accgggtcgg gagccagcac
     3301 catgatgagg tcggcctctt caaccgcagc gtcaggtgtg agcacccgca ggccctcggc
     3361 ctcggccttg ccgcggctct tcgaaccttc gagcagcccg acgcgcacgt ctacgccaga
     3421 gtcgcgcagc gacaacgcat gcgcatgccc ctggctgccg tagccaagca cagcgacgtg
     3481 gcggttttgg atgatcgaca gatcggtgtc gtcgtcgtag aagatctcaa caggcacggg
     3541 acgtgcgctc cttagctcgg ttcttgcttg atgtgcagcg ggttttggga atgtagaggg
     3601 atttcaggcg atccggtcga ccggacgcag cgtgcgatcg gtgatcgacc gcgagccgcg
     3661 acccatcgcc accatgcccg actggacgag ttctttgaca ccgaacggct cgagcacccg
     3721 caggaacgca tcgatctttt cggcaccgcc ggtggcctcg atggtcacgg catcggcagc
     3781 gacgtcgatc accttc
//
LOCUS       DASWVF010000336         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_187418, whole genome shotgun sequence.
ACCESSION   DASWVF010000336 DASWVF010000000
VERSION     DASWVF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187418"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..557)
                     /locus_tag="VGD55_05990"
     CDS             complement(69..557)
                     /locus_tag="VGD55_05990"
                     /inference="COORDINATES: protein motif:HMM:NF022736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEY0869926.1"
                     /translation="MAPPVFHERRYVPWWWWVVGAVIAIPTTEGVVVLGPEMTGHPSA
                     LLAIGCLVATVVLEAVFLTMLGRSDVDVDETGIRAGRATLAANHLGRARALDAVSAHR
                     LLGPGLRADAELSLRPWIKSAVQIEVEDDGDRTPYWVVATRRPAELVTALDNVRRAKA
                     EL"
     gene            637..1263
                     /locus_tag="VGD55_05995"
     CDS             637..1263
                     /locus_tag="VGD55_05995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0869927.1"
                     /translation="MIEQASGQRPAPGLLAPLVLPPDVRPPIAAADAPAPGAILGQHY
                     ARCYGCGAAQPAGLHMTFAVGDDVRVISEFVVTPDHEGAPGLAHGGILTAALDETQAT
                     LLWLLRKPAVTARLETDFVAPVPVGSIVRIEARCLGLADRKIYTSAEGLLVAASGDTD
                     GTLALRSAALFITVPVEHFTAFGRADLVEASMNRSSEAGPIARIEVNP"
     gene            1260..1727
                     /gene="dut"
                     /locus_tag="VGD55_06000"
     CDS             1260..1727
                     /gene="dut"
                     /locus_tag="VGD55_06000"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0869928.1"
                     /translation="MKGLPVLVRRLYPDVELPTYAHPGDAGADLVTTQAAELAPGERI
                     VLPTGIAIALPDGYAAFVHPRSGLAARYGVALVNAPGTIDAGYRGEIKVIMVNLDPRE
                     SVSFAVGDRICQLVIQRVERAEFHEVAELPGSHRGVGGHGSTGGFSGAQAGGS"
     gene            1727..2461
                     /locus_tag="VGD55_06005"
     CDS             1727..2461
                     /locus_tag="VGD55_06005"
                     /inference="COORDINATES: protein motif:HMM:NF023917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEY0869929.1"
                     /translation="MAFGRGKKGSGSDGVDDDAEAAELDAAEDVGTVEIVVARAPIDE
                     ADAGTDDVLPRIDLGGLRVPVLPGTEVRVELNEAQFPIAATIVHGASSLQVLAFAAPR
                     NSGIWEEVRTEIADSVRAEGGHVDEVDAVFGPELRTRVRGEAQQGKATEQVLRFVGFD
                     GPRWFVRGVFSGPAATHPAQATVLETVLTSIVVVRGTDPMAPRDPLPLRLPKEALAAA
                     DPAAAAAAGPPVLEPRDRSREITETS"
     gene            2483..2914
                     /locus_tag="VGD55_06010"
     CDS             2483..2914
                     /locus_tag="VGD55_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0869930.1"
                     /translation="MHEIEPDAATDASDYDTAGAAGPGVLRRALHRLTTPEHILEAEN
                     RQEQSLEGGGTPVERCRDRAQVCVVGMLRTVTIQPRGGSPSLQAELWDGSGSVDVVWI
                     GRRRIPGIEPGRVIRAEGRMTLRDGRRVMFNPRYELRPSGS"
     gene            complement(2949..3611)
                     /locus_tag="VGD55_06015"
     CDS             complement(2949..3611)
                     /locus_tag="VGD55_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY0869931.1"
                     /translation="MRVAIAGAGAVGRSIAGELVENGHEVLVIDKDSRAIDVDAIPGA
                     EWVLADACEISNLDELGLDRCQVAVAATGDDKVNLVVSLLAKTEYGVPRVVARVNDPV
                     NEWLFDESWGVDVAVSTPRLLSALVEEAVSVGDLVRLMTFRQGQANLVEFTLPPTGRY
                     VGQRVGSVPWPADSALVVILRDGRVIVPSSDDPLEGGDELLFVASPNVEDELRGLLLH
                     GE"
     gene            complement(3611..>4218)
                     /locus_tag="VGD55_06020"
     CDS             complement(3611..>4218)
                     /locus_tag="VGD55_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY0869932.1"
                     /translation="ELCHSVAIIDTDAAAFRRLPPHFRGRKIHGVGLHRSVLEDSGIR
                     QAGAFAAVSSGDNSNIIAARVARETFGVENVVARIYDIRRAEIYQRLGIPTVATVRWT
                     ADQMLRRVLQEGSQALWRDPTGTVQLAEVHVDQAWVGHRLSELESAAGVRVAFVTRFG
                     EGVLPGHDTVFQEGDLLNVIVRDKDAEAATSTLGKVPEESS"
BASE COUNT          677 a         1393 c         1427 g          721 t
ORIGIN      
        1 ggcgatctgc gcggcgatgg tcgcggcgat agccgtgtaa atcttcagcg cgatcgattc
       61 agacttgctc ataactccgc cttcgcacgt cgcacgttgt cgagtgcggt tactaattca
      121 gctggacgcc gagtggcgac cacccagtat ggagtgcggt ctccgtcgtc ctcgacttcg
      181 atctgcacgg cgctcttgat ccatggacgc agcgaaagct ctgcgtcggc gcgaaggccg
      241 ggtccgagca gtcggtgtgc actaacggca tcgagtgctc gggcccgacc gaggtggttg
      301 gcagccagcg tcgcgcgccc ggcgcggatg cctgtttcgt cgacgtcgac gtccgacctg
      361 ccgagcatcg tcaagaacac ggcttcgaga acgactgtcg cgacaaggca cccgatcgcg
      421 agcagtgcgc ttgggtgacc ggtcatctcg ggaccaagaa caaccacgcc ctcggttgtc
      481 ggtatcgcga tcaccgcacc gacgacccac caccaccacg gcacgtagcg tcgctcgtgg
      541 aagacgggtg gggccatggc gccaaggtta cgcagcgagc gggcggttga gaccaccgca
      601 ggcgtcggcc ggtagtctcg cccttcgtga ccgatcgtga ttgagcaggc cagcgggcag
      661 cgtccggcac ctggtctgct cgcgccgctc gtcttgccgc ctgatgtgcg gccacctatc
      721 gccgccgcag acgcccctgc tcccggcgcg attctgggtc agcactacgc gcgctgctac
      781 ggatgcggcg ccgcccagcc agccggtctg cacatgacat tcgcggtcgg tgacgacgtc
      841 cgggtcatct ctgagtttgt tgtgacaccc gaccacgaag gtgcgcccgg actcgcgcac
      901 ggcgggattc tgaccgccgc gctcgacgag acccaagcaa cactgctgtg gttgctgcgc
      961 aagccggcgg tgaccgcgcg gctcgaaacc gacttcgtcg cgccggtgcc agtcggatcc
     1021 atcgtgcgga tcgaagctcg ctgccttggc cttgccgacc gcaagatcta caccagtgcc
     1081 gagggtctgc tcgttgccgc gtctggtgac accgacggca cgcttgcgct tcggtcggcg
     1141 gcgctgttca tcaccgttcc cgtcgaacat ttcaccgcgt tcggtcgcgc cgacctcgtc
     1201 gaggcttcga tgaacaggtc gagcgaggcc gggccgatcg cgcggatcga agtgaatccg
     1261 tgaagggcct gccggttctc gtgcggcgtc tctatccgga cgtcgagttg ccgacgtatg
     1321 cgcatccggg tgacgccggt gccgatctgg tcacgacaca ggcggctgag ctcgcgcctg
     1381 gcgaacggat cgtgctgccg actgggatcg ccatcgcgct gcccgacgga tatgccgcgt
     1441 tcgttcatcc gcggtcaggg ctcgctgcgc gttacggcgt tgcgttggtc aacgctccgg
     1501 gcacgatcga cgcggggtac cgtggtgaga tcaaggtgat catggtgaat ctcgacccgc
     1561 gtgagtccgt cagcttcgcg gtcggcgacc gcatctgcca actcgtgatc caacgcgtcg
     1621 aacgcgccga gttccacgaa gtcgccgaac ttccgggttc acaccgtggt gtaggcggtc
     1681 acggttcgac cggtggattc agtggcgcgc aggcaggtgg ttcgtgatgg cgtttggccg
     1741 gggcaagaaa ggctcgggtt ccgacggcgt cgacgacgac gcagaagcgg ccgaactcga
     1801 cgctgccgag gacgtaggca cggttgagat cgtcgttgct cgcgctccga tcgatgaagc
     1861 cgacgcgggt accgacgatg tgttgccgag gatcgacctc ggcgggttgc gcgtgccggt
     1921 gctgcccggc accgaggtgc gcgtcgaatt gaacgaagca cagtttccga ttgccgcgac
     1981 catcgtgcac ggtgctagtt cgctccaggt gcttgcgttc gcggcgccgc gcaacagcgg
     2041 catctgggaa gaggtccgca ccgaaattgc cgactcggtg cgcgctgaag gcgggcacgt
     2101 cgacgaagtc gacgcagtgt tcggccctga gttgcgcact cgcgttcgcg gggaggcgca
     2161 acaggggaag gccaccgaac aggtcctgcg attcgtcgga ttcgatggtc cgcgctggtt
     2221 cgtgcgcggc gtattcagtg ggcctgccgc gacccatccg gcccaggcca ccgtgctcga
     2281 gacggtactt acgagcatcg tcgtggtgcg cggtacagat ccgatggcgc cgcgcgatcc
     2341 gctgccgctg cgactgccca aggaggcact tgcggcggcc gacccggcgg cagcggcagc
     2401 ggctggccca cccgtcctcg aaccgcgcga tcgcagccgc gagatcaccg agacgagctg
     2461 acgggcaggt atcctcaaag ctatgcacga gatcgaacct gacgccgcga ccgacgccag
     2521 tgactatgac accgccggtg cggcggggcc cggtgtgctg cgtagggcct tgcaccggct
     2581 cacgacgccc gagcacattc tcgaggccga aaaccggcag gagcagtcgc tggaaggtgg
     2641 cggcacacct gtcgaacgct gccgtgaccg tgcccaggtc tgcgtcgtcg gcatgttgcg
     2701 gaccgtcacg atccaaccgc gcggcggatc gccgtccctg caagcagaat tgtgggacgg
     2761 atcgggctca gtcgacgtcg tgtggatcgg tcggcgccgg atcccgggca tcgaacctgg
     2821 ccgcgtcatc agggctgagg gccggatgac actgcgcgac ggccggaggg tgatgttcaa
     2881 cccgaggtac gagttgcggc cttccgggtc ttagtcacgt ccgggtctta gtcacgtccg
     2941 ggtctcagtt actccccgtg cagtagcagt ccgcgcagtt cgtcctcgac gttcggcgac
     3001 gcaacgaaaa gcagctcgtc gccaccctcg agcgggtcgt cgctgctcgg cacgatgacc
     3061 cggccgtcgc gcaggatcac cacgagggca ctgtcagccg gccacggcac cgagccgacc
     3121 cgctggccga cgtagcgacc ggtgggcggc aaggtgaact cgacgaggtt cgcctggcct
     3181 tggcggaacg tcatcagccg caccaggtcg cccacgctga cggcttcctc gaccagcgcc
     3241 gagagcaggc gtggggtcga cacggcgacg tcgacgcccc acgactcgtc gaacagccat
     3301 tcgttgaccg gatcgttgac ccgagccacg actcgcggaa ccccgtactc ggtcttggcg
     3361 agcagtgaga cgaccaggtt gaccttgtcg tctccggtcg cagcgaccgc gacctggcac
     3421 cggtcgaggc cgagttcgtc gaggttggag atctcgcacg catcggccag cacccactcg
     3481 gcaccgggga tcgcatcgac gtcgatcgcg cggctgtcct tgtcgatgac cagcacctca
     3541 tgcccgttct cgacgagttc tcctgcgatc gagcgtccga cggcgccggc tccggcaatg
     3601 gcgacccgca tcagctgctc tcctccggaa ccttcccgag cgtgctggtg gcagcctcag
     3661 cgtccttgtc tcgcacgatc acgttgagca ggtcaccttc ctggaacacg gtgtcgtgcc
     3721 cgggcaaaac gccttcgccg aagcgagtta cgaacgcgac gcggacgccc gcagccgact
     3781 cgagctccga caaccggtgc cccacccatg cctggtcgac gtgcacctcg gcaagttgca
     3841 ccgtgccggt ggggtcgcgc cacagtgcct gggagccctc ttgcagcacc cgccgaagca
     3901 tctggtcggc ggtccagcga acggtcgcga ccgtcgggat gcccaggcgc tggtagatct
     3961 cggcgcggcg gatgtcatag atgcgggcca cgacgttttc gacgccgaag gtctcgcggg
     4021 ccacgcgggc ggcgatgatg ttggagttgt cgccgctgct gactgccgcg aacgcgccgg
     4081 cctgccggat gcctgagtcc tcgagcacgc tgcggtgcag gccgaccccg tggatcttcc
     4141 ggccacggaa atggggcggt aagcgccgga atgccgcggc gtcggtgtcg atgatggcga
     4201 cactgtggca cagttctt
//
LOCUS       DASWVF010000337         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_187426, whole genome shotgun sequence.
ACCESSION   DASWVF010000337 DASWVF010000000
VERSION     DASWVF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187426"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VGD55_06025"
     CDS             <1..1482
                     /locus_tag="VGD55_06025"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEY0869933.1"
                     /translation="ADTGAEPTDATAGADSVTVKVVDTQVGQIAPDGTYQPTPAGHPI
                     EYDYQLTKDTKENGEWRITNAPPYLVLTVSQIESSYQQGYVYFLRPDEQMLVPVRVFL
                     PVTRDQFAGALLNTLRAGPPAWLKQAVTTALPPSTSTATSEPTQVNGVTTVDLPRTVA
                     NLPAAQRNAIDAQISYTLSNTATPQQDFGSLRILAGGQPLISNPQLALQSATDWQSFD
                     PDFLRVDFYYSDVDHLTHDHLGRLVAGDTGILGLTNLLAPVVAPRSSVGGNGELIAGV
                     VQESPNTQALYAGPLLAPKKLLSGSAFTTPSWDSLGNLWTVQQQTSTSAPQVRIAPSG
                     TATLPGPVAAPLLANLVIKQLKISRDGTRVAVLAVSTNVSQVLVGAVANDGSTIGHFY
                     PVAPSLTSVTDFAWASSTTLDILGTVPNSNEPGTSSTLWSVDIDGWAPDGPTIEPVPT
                     TAVSIAAAPGRPAVVGTSGNQIEEYDGGEWQFVANGSAPHYPG"
     gene            1575..2282
                     /locus_tag="VGD55_06030"
     CDS             1575..2282
                     /locus_tag="VGD55_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0869934.1"
                     /translation="MNSFAAVLDLVLPSNCAGCDRSDDALPRLRGLCVDCVATVRQAA
                     PLEWALSSKVRAFAGAAYDGVIRSILLNYKEHGRLCLRRELGGCLTVSVLAAARFSAG
                     RDLLLVPAPSAAAAQRERGHDPVGAMARTVVGQLRAIGIQIGMAAALRQERAVADQSG
                     LDVVARQENLAGALVVTNTAQVRGRRVVVVDDIVTTGATALEAARALTAAGAVVEAVA
                     CVAATPRRFPIGSGAAP"
     gene            2736..3488
                     /gene="raiA"
                     /locus_tag="VGD55_06035"
     CDS             2736..3488
                     /gene="raiA"
                     /locus_tag="VGD55_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0869935.1"
                     /translation="MEIVVTGRHLDVPAQLRTHAEEKLAKVVKLAPKAFRVDVEVSRE
                     RNPRMSEICERVELTCVSPGPVIRAEAAAHEMLEAFDLAWAKLESRLRKAADRRRVHR
                     GARTPISVATATRGLVESPVESRVEAAESEDPYRLAEPPGPIVVREKLHKATPMTLDD
                     ALYEMELVGHPFFLFVESLSGLPSVVYRRHGYDYGVIRIDTADTPVAADQAARATNNA
                     RAARATDNAGAARATNNARAARQTLAASNGHG"
     gene            3595..4392
                     /locus_tag="VGD55_06040"
     CDS             3595..4392
                     /locus_tag="VGD55_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869936.1"
                     /translation="MSDRQQGMADVHVLPDRGERHRNDAGDAGAAGVVVAGELSVTLK
                     ATEPIRVLVVDDHALFRRGLEMVLAQEPDIELCGEASDGAEAVAKAAETLPDIVLMDV
                     RMPKRSGIEACTAIKDVVPSAKIIMLTISDEEADLYDAIKAGAMGYLLKEISIDEVAA
                     AIRAVHGGQSLISPSMASKLLSEFASMIKKGDSRQQVPAPKLTDREMEVLRLVAKGLN
                     NRDIAKQLFISENTVKNHVRNILEKLQLHSRMEAVVYAVREKLLEIT"
     gene            complement(4416..>4693)
                     /locus_tag="VGD55_06045"
     CDS             complement(4416..>4693)
                     /locus_tag="VGD55_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY0869937.1"
                     /translation="SDGRAHGMLAITTFLAIIVAAARLPQLVHAIGGWHGVATASVVI
                     AWAMVASMVAMIASRRSAGNSGYFGMAERVFYVAIVAWLIAVGVALM"
BASE COUNT          815 a         1563 c         1561 g          754 t
ORIGIN      
        1 gccgacaccg gcgcggagcc aaccgacgcc acggcgggtg ccgattcggt cacggtcaag
       61 gtcgtcgaca cccaggtcgg gcagatcgcc ccggacggca cctaccagcc gacaccggcc
      121 gggcatccga tcgagtacga ctatcagctc acgaaagaca cgaaggaaaa cggtgaatgg
      181 cgcatcacga acgcgccgcc gtacctcgtg ctgacggtgt ctcagatcga gagttcctac
      241 cagcaggggt atgtgtactt cctgcgaccc gacgagcaga tgctcgtgcc cgtgcgggtg
      301 ttcttgcccg tcacccgtga tcagttcgcg ggcgctttgc tcaacacgtt gagggccggg
      361 ccccccgctt ggttgaagca ggcggtcacg acggcgctgc cgccaagcac gagcacggcg
      421 acgtcggagc caacgcaggt caacggcgtc accaccgtcg acttgccacg taccgttgcc
      481 aacctgccgg cagcacagcg caacgcgatc gatgcgcaga tcagctacac cctttcgaac
      541 acggcgaccc cgcagcagga cttcggcagc ctgcggatcc tggctggcgg gcagcctttg
      601 atcagcaacc cgcaactcgc gttgcagtcg gccaccgact ggcagtcgtt cgaccccgac
      661 ttcttgcggg tcgacttcta ctacagcgat gtcgaccacc tcacgcatga ccatcttggc
      721 cgtctcgtcg cgggcgacac cggcattctc ggcctcacga acttgctggc acctgttgtc
      781 gcgccgcgca gctcagttgg cggtaacggc gagctgattg ccggcgttgt gcaggagtca
      841 ccaaacacgc aggcgctgta cgccggtccg ctgctcgcgc cgaagaaact gctgagcggc
      901 agcgccttca cgacgccgtc gtgggattcc ctgggcaacc tatggacagt gcagcagcaa
      961 acgagcacca gcgcgcctca ggtgcgaatc gctccctcgg gcaccgcgac gttgccgggg
     1021 ccagtggctg cgcccttgct tgccaacctg gtgatcaagc agctgaagat ctcccgcgac
     1081 gggaccaggg tggcggtgct tgccgtctcg acgaacgtca gccaggtgtt ggtcggcgcg
     1141 gtcgcgaacg acggctccac gatcgggcac ttctatccgg tcgcaccatc gttgacctcg
     1201 gtgacagatt tcgcgtgggc gtcgtcgacc acgctcgaca ttctcggaac cgtgccgaac
     1261 agcaacgagc ctggcacctc cagcacgttg tggagcgtcg acatcgatgg ctgggcaccc
     1321 gatggcccga cgatcgaacc ggtgccgacc actgcggtgt cgattgccgc tgcgccgggc
     1381 cggccggccg tcgtcggaac gtcgggcaac cagatcgagg agtacgacgg cggggagtgg
     1441 cagttcgtgg ccaacggctc cgcgccccac tatccaggct gagtctcacc cgcttgcctg
     1501 tggacgacgt gaagtcgctc cacagatctg tcgcgtgagt cgtgccgcgt ccgtcgcggc
     1561 gcgaggctgt ggccatgaac tcgtttgctg ccgtcctcga cctcgtgttg ccgtcgaact
     1621 gcgccgggtg tgatcgcagc gacgacgcgc tgccgcgatt gcgcgggctc tgcgtcgact
     1681 gcgttgcgac ggttcggcag gcggcaccgc tggagtgggc actctccagc aaagtgcgcg
     1741 cgttcgctgg cgccgcatac gacggtgtga tccgctcgat cttgctcaac tacaaggagc
     1801 acggccgcct gtgtctgcgc cgcgagctcg gcggatgctt gacggtgagc gtgctcgcgg
     1861 ccgcgcgctt cagtgcaggt cgcgacttgt tgctcgtgcc ggctccatcc gcggccgcgg
     1921 cccagcgcga acgaggccac gatcccgtcg gcgcgatggc tcgcacggtc gtcgggcagc
     1981 ttcgtgcgat cggaatccag atcgggatgg cagcggcatt gcggcaggag cgcgcggtcg
     2041 ccgatcaatc aggcctcgac gtcgtcgcac ggcaggagaa tctcgccggt gctctggtcg
     2101 tcacgaacac tgcacaggtg cgcggacggc gggtggtggt cgtcgacgac atcgtgacaa
     2161 cgggggcaac ggctctcgag gcggcgcgag cgttgaccgc tgccggcgcg gtcgtcgagg
     2221 cagtcgcgtg cgtcgcggca acgcctcgcc gattcccgat tgggagcggt gccgcgccct
     2281 gaccgcagcc agacaggctt cgcaagactc gtggctcggg ctaggttggc aaggtggcgt
     2341 ccgcccgggt ccgtggttgc gtcggcaggt tctcctgccg gctagccgat gccagtcgca
     2401 ggctaaacgg tccacgtaag gcgactcgtc gtcgtccgat cacggtgcgg cttagaggta
     2461 agtcctgcct cgccggcggt ccacatggct gccgaccgag agaaggtcag tagtggcaac
     2521 gatgcgcaac tgcgcgctgc gagcacctcg gcaggcggat gtggggtcga agaccaggtc
     2581 ggccgggcgg gcgtctcacc cggccgtgcc agtacgtccg cgttcggtcg agtgcggccg
     2641 tcgatacggg gaaggaaggt agcgaccgtc accatcgccg tccgggcaaa caccgttcgg
     2701 ggcggctggg ggtcttagag acgggagaat ccaaggtgga gatcgtggtc accggccggc
     2761 acctcgacgt cccggcgcag ctgcggacac atgccgagga gaagctcgcc aaggtcgtga
     2821 aactcgcgcc gaaggcgttt cgggtcgacg tcgaggtctc ccgcgagaga aacccgcgga
     2881 tgtcggagat ttgtgaacgc gtcgaactga cctgcgtgtc gcccgggccg gtgatccgag
     2941 ccgaggccgc ggctcacgaa atgctcgaag cgttcgacct ggcctgggcc aaactcgaat
     3001 cgcggttgcg caaagcagct gaccggcggc gggtgcatcg aggcgcccgt acaccgatat
     3061 ccgtggcgac cgcgaccaga ggactggtcg agagtccggt cgagagccgc gtcgaggccg
     3121 ccgaatcgga agacccgtac cgcctcgccg agccgcccgg gccgatcgtg gtccgcgaaa
     3181 agctgcacaa ggcaacgccg atgaccctcg atgacgccct ctacgagatg gaactcgtcg
     3241 gccacccgtt cttcctgttc gtcgaatcgc tcagcgggct gccgagcgtc gtctatcgcc
     3301 gccacgggta tgactacggc gtgatccgca ttgacacagc tgacacgccc gtcgcggccg
     3361 atcaggccgc tcgcgccacc aacaatgctc gcgccgctcg cgccaccgac aatgctggcg
     3421 ccgctcgcgc caccaacaat gctcgcgccg ctcgccagac cctcgccgcc tcgaacgggc
     3481 acgggtgacg cgtctgcgtt acgcatccgg cgtttgccgc gccgacggca cgggcagcca
     3541 caagtcgcag cccctcgtgt ccgtaccatg ggtgcggggt taccgggagg cgacgtgagc
     3601 gacaggcagc agggcatggc cgacgtccac gtgcttcccg atcggggtga gcgccaccgc
     3661 aacgatgcgg gcgacgctgg ggctgccggt gttgtcgtcg ccggagagct gtccgtgact
     3721 ctgaaggcca ccgagccgat ccgggtgctg gtcgtcgatg accacgcgct atttcggcgg
     3781 ggccttgaga tggtgctggc ccaggagccc gacatcgagt tgtgtggcga ggcaagtgat
     3841 ggcgccgagg ccgtcgccaa agccgccgag accctgcccg acatcgtgtt gatggacgtg
     3901 cggatgccca agcgcagcgg catcgaagcc tgcaccgcga tcaaggacgt cgtcccctcg
     3961 gcgaagatca tcatgctgac gatcagcgat gaggaagccg acctctacga cgccatcaag
     4021 gccggcgcta tgggttacct gctgaaggaa atctcgatcg acgaggtcgc agcagcgatc
     4081 cgcgccgtcc acggcgggca gtcgttgatc agcccgtcga tggcgtccaa gctgctcagc
     4141 gagttcgcct ccatgatcaa gaagggcgac tcccgtcagc aggtgccggc acccaagctc
     4201 accgaccgcg agatggaagt gctgcggctc gtcgcgaagg gcctcaacaa ccgtgacatc
     4261 gccaagcagc tgttcatcag cgagaacacc gtcaagaacc acgtgcgcaa catcttggag
     4321 aagttgcagc tgcactcgcg catggaagcg gtggtctacg ccgtccgcga gaagcttctc
     4381 gaaatcacgt agtcattgct ttcgtcgccg cggagtcaca tcagcgcaac gccgaccgcg
     4441 atcagccacg ccacgattgc cacgtaaaac acgcgctcgg ccatcccgaa gtagccactg
     4501 ttgcctgccg accgtcgcga cgcgatcatc gccaccatcg acgcgaccat cgcccaggcg
     4561 atcaccacgc ttgccgttgc aacgccgtgc cagccaccga tggcgtgcac gagctgcggc
     4621 agccgagccg ctgcgacgat gatggctagg aaggtcgtta tcgcaagcat cccgtgagcg
     4681 cggccgtcgc tcg
//
LOCUS       DASWVF010000338         7432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_289211, whole genome shotgun sequence.
ACCESSION   DASWVF010000338 DASWVF010000000
VERSION     DASWVF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289211"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VGD55_06050"
     CDS             complement(<1..422)
                     /locus_tag="VGD55_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0869938.1"
                     /translation="MPGVMVDEERRQVFDEFARARMPELLRFGHALTGSPHAAADLVQ
                     DALERTMLAWARVENQGDPEGYVRRIMVNRNISIWRRRRRETLVLDVPEDAGRRGAGS
                     FEPVVRDLALWDRIRALPPRQRTVIVLRYYEDMSEAQIA"
     gene            complement(484..2001)
                     /locus_tag="VGD55_06055"
     CDS             complement(484..2001)
                     /locus_tag="VGD55_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0869939.1"
                     /translation="MTSTTPSAAASSSSAGASVISAEPAVSSAAEVADLDALAKLLGS
                     RLVRPSDSGYTAASRLYSTRFDGVKPAAVARCASVQDVQHCLDFVRATGTPVAARSGG
                     HSFAGYSTTSGLVIDVGPMKQVQMIGANTARVGAGAALIDVYAGLATQNQSVPGGSCP
                     TVGITGLTLGGGIGVVARKYGLSCDAVTAVELVTVDGKSIRCSENSDADLFWAHRGGG
                     GGNFGIVTALEFQTHPTQTLTHFLVRWDWSHAHDVVTAWQQWLPTTPNELWSSLHIDG
                     AGSASDQPHVYASGVYVGPTSGLAPLLDALQSASKAPFTTRFVKEDGYLSTMFVEGGC
                     ANLTTLACHRADIDPGGQLGRESNAARSDFIAEALAPAGVDVLLTGIEARRGLGLPGG
                     SVLFDAYGGAINSVAPGDTAFVHRDKLACLQYVAPWGASASPSVVAANQAWLDGVYGQ
                     MRPYVSGFAYLNYIDAKLADWQHAYYGANLQRLIGVKAAADPSDLLHFAQGLPAS"
     gene            2351..3097
                     /locus_tag="VGD55_06060"
     CDS             2351..3097
                     /locus_tag="VGD55_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869940.1"
                     /translation="MIGSVVVLVSGCRTSAVSAKGSEPSAAATVSSSTPALTPTVTPT
                     LPPTPTPTPDPTPTKKKYGFGLPSGPTAPGPELLVYAPLQQGDCAAAQTSLGAPDQSG
                     NWSSLPDPAKVLLFQAGVEACTGDLTTAGSLLALDRTLFGATTTEDPQDADSACALYK
                     ALVSVLDQVDPASAQCPTGAEPAWQFDANSNPLDPRTHQDAYAHPATSSTPTPAPSAS
                     TASPTAHPSHILVHPSLILGHSLVTPSATS"
     gene            3105..4064
                     /locus_tag="VGD55_06065"
     CDS             3105..4064
                     /locus_tag="VGD55_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869941.1"
                     /translation="MPEQERPATESVDVSDAAMAGHEVTASPAAAPPAGETSGGAFVG
                     LRPLVALLGNVTVLTSLLYFFGWRQTATQDSDLRLNQDIFGLSTQDYLLRSVGSVIRL
                     LSVLGVVGLLFVYLDHALRWAVAHYGPRDRIVLIAVRSLDVAWLVVPVVVYLLRLWRP
                     DSSDLYIAYPLALGAGVMLIVYRAQVRRRLGLRTDPTDGRSLLERGAVALLLVVAVFW
                     ATSNYADVEGHRLAEGFRTSDLIPVTVFSPQRIDLSGAQEQPISASSTDPAGALYQYR
                     YTGLRLMAHSGGHYFLVPASWTPTSGALVVLSDKDPVRLEFGS"
     gene            4182..5117
                     /locus_tag="VGD55_06070"
     CDS             4182..5117
                     /locus_tag="VGD55_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0869942.1"
                     /translation="MSVIEVTGLRKVYGERTVVDGVSFSVEPGEIFGILGPNGAGKTT
                     TVECIGGLRHRDGGSITVAGMDPAKEGSAFRQVLGIQLQESRLPEKMTVIEALDLYAS
                     FYPSPADPAELLDRLGMVEQRKTYFGKLSGGQKQRLSVALALIGNPRVAILDELTTGL
                     DPAARRDVWDLLEDLRAGGVTLLLVSHFMEEAERLCDRVIVIDHGKIIAADTPEGLAA
                     SVQADQRMSFLPSTPFDLAVLRALPDVHSVNTVGERVVVTGTDNVASAVILGLHGQGI
                     WVGKLRVDQPSLDDAFVALTTSNQAPENSSTEASR"
     gene            5114..5911
                     /locus_tag="VGD55_06075"
     CDS             5114..5911
                     /locus_tag="VGD55_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0869943.1"
                     /translation="MSAASSTKPVTTARMIPAINWRAARAMLVSEGRLLLRNPGVVIW
                     TAVLPVAAAIILGSIPAVRKPSASFGGLSFFQVYQPILVFFALTLLAMQALPDVLTRY
                     REMGVLKRLRTTPVSPALLLCAQLTLILSVAIICGALMVVAPPLGGAPWPRNIGGFVI
                     SYLLGAWAMLGLGMVIASLFHNAKVAAGFGSFLFFVLQFFAGLWWQRPNMPTWLRDIS
                     NFTPSGAAVQALTNSSVGHWPGALYLIVLAAWGTVTSWLAVRFFSWE"
     gene            5976..6170
                     /locus_tag="VGD55_06080"
     CDS             5976..6170
                     /locus_tag="VGD55_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HEY0869944.1"
                     /translation="MRLTDFWWRMRQHFGPTYAESFAHDFVLGELGGRTIDEALAQGV
                     DVQEIWRAVCRTVEVAPKLR"
     gene            6410..>7432
                     /gene="recA"
                     /locus_tag="VGD55_06085"
     CDS             6410..>7432
                     /gene="recA"
                     /locus_tag="VGD55_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0869945.1"
                     /translation="MAAPDRAKALENALAQIERAHGKGSVMRLGDEVREPIEVIPTGS
                     IALDVALGIGGLPRGRIVEIYGPESSGKTTLALHAVANAQRAGGIAAFIDAEHALDPE
                     YAAKLGVDSDALLVSQPDTGEQALEIADTLIRSGAIDIIVIDSVAALVPRAEIEGEMG
                     DSHVGLQARLMSQALRKITGALSNSGTTAIFINQLREKIGVFFGCFNYNTRVTLADGT
                     QEKIGKIVNQKMAVEVLSYDPETDRIVPRKIVNWFDNGPADEFLQFTVAKSGKNGRAQ
                     FAATANHLIRTPGGWRPAGEFLAGDRVMVTEPHRLSSQQMQVVLGSLMGDGNLSPNRR
                     GRSGVRF"
BASE COUNT         1201 a         2365 c         2478 g         1388 t
ORIGIN      
        1 gcgatctgcg cctcggacat gtcttcgtag tagcgcaaca cgatgacggt tcgctggcgc
       61 ggtggcagcg cgcgaatcct gtcccacaag gccaagtcgc ggaccactgg ttcgaagctg
      121 ccagcgccgc ggcgtccggc atcttctgga acgtccaaga ccagcgtctc gcgacgccga
      181 cgtcgccaga tgctgatgtt gcggttcacc atgatgcgcc gcacataccc ctccggatcg
      241 ccctggttct ccacccgcgc ccacgcgagc atcgtgcgct cgagcgcgtc ctggacgagg
      301 tcagccgcgg catgggggct tccggtcaaa gcgtgaccga aacgcagaag ctccggcatg
      361 cgggcgcggg cgaactcgtc aaagacctgt cgccgctcct cgtccaccat caccccgggc
      421 atctcgtacg cagtgacgac gccggacgcc gtcgctgggt tgcatgggct tgcatgagcc
      481 ctttcagcta gcaggcaggc cctgcgcaaa gtgcaacaga tcggacgggt cggccgcagc
      541 tttgacgccg atcagccgct ggaggttcgc accgtaatag gcgtgctgcc agtcggccag
      601 cttggcgtcg atgtagttga ggtaggcgaa gccgctcacg tagggccgca tctggccgta
      661 gaccccgtcg agccacgcct ggttggcggc gacgaccgac ggcgaggcgc tcgcgcccca
      721 gggcgccacg tattgcaggc aggccaactt gtcgcggtgc acgaaggcgg tgtcgccggg
      781 tgcgacggag ttgatcgcgc cgccgtaagc gtcgaacagc acgctcccgc cgggtaggcc
      841 caatccgcga cgggcctcga tgccggtcag cagcacatcg actcccgcgg gcgcgagcgc
      901 ttcggcaatg aagtcggagc gggcggcatt cgattcgcgg ccgagttgtc cgccagggtc
      961 gatgtcggcg cgatggcacg cgagcgtcgt caggttcgcg cagccgccct cgacgaacat
     1021 cgtcgatagg tagccgtcct ctttcacgaa tcgcgtggtg aaaggcgcct tcgacgccga
     1081 ctgcaaagcg tcgagcagtg gcgcaaggcc actcgtcggg ccgacgtata ccccgcttgc
     1141 atagacatgc ggttgatcgg acgccgaccc ggcaccgtcg atgtgcaggc tcgaccatag
     1201 ttcgttcgga gttgtcggga gccattgctg ccaggccgtc acgacatcgt gcgcatgcga
     1261 ccaatcccac ctgacaagga aatgcgtcag ggtctgtgtt gggtgtgttt ggaactccaa
     1321 cgcggtgacg attccgaagt tgccgccacc acctccgcga tgcgcccaga agaggtcggc
     1381 atcgctgttt tcgctgcacc gtatggactt tccgtcgacc gtgacgagtt cgacggcggt
     1441 gactgcgtcg caactcaacc cgtactttcg agccacgacg ccgataccgc cgccgagggt
     1501 caaaccggtg atgccaaccg tcgggcacga gccgccgggc accgattggt tctgggtggc
     1561 gagccccgcg tacacatcga tgagggccgc acccgcgccg actcgtgcgg tgttggcgcc
     1621 gatcatctgc acctgcttca tcgggcccac gtcgatcacc aggcccgacg tcgtcgagta
     1681 gcccgcgaag ctgtgccctc cactgcgcgc ggcgaccgga gtgccggtag cacgcacgaa
     1741 gtcgaggcag tgctgcacgt cttgcaccga cgcacagcgc gcgactgcag cgggtttcac
     1801 accgtcaaac cgagtcgaat acaggcggga cgccgcggtg tagccgctat cggacgggcg
     1861 cacgagtcgg ctcccgagga gcttcgccag ggcgtcgaga tcggcgacct cggccgccga
     1921 cgacactgcg ggctcggcag atattactga ggcacccgcg ctcgaagacg atgcggccgc
     1981 gctgggggtt gtactcgtca agggtgacga tgccggggtg cccgacccgc tcagggtcgg
     2041 cgtcccgctt gggggtgttg ttcgacttgt gcagccctcg atcgcgccga cagcgccgat
     2101 caccgccgcg gcaccagcga gccgcaggaa ctcgcgccga tcgatggggt cctcccagcg
     2161 gggttatgcg tggcggttcc gcgccgcagt ctgctcttcg atgacgtcgg gatcgacgat
     2221 ttgggtgtct cgaggctagc gagtcgttga catatcgggc gatccggcgt atctcggggg
     2281 cagccgggtg ctcttcaagg cgtgaactgc cggaactccc gtaggagcgg ccatgcgtaa
     2341 aggcgtgctc gtgatcggtt ccgttgtcgt gctcgtgtcg ggttgccgga ctagcgccgt
     2401 gtcggccaag gggagtgaac cctcagctgc tgcgacggtc tcaagttcga cgccggcact
     2461 cacgcccacg gtcacgccta ccctgccgcc aacgccgact ccgaccccag acccgactcc
     2521 cacgaagaag aagtacggct ttgggttgcc gagtggcccg acggcaccgg gacctgagct
     2581 gctcgtctat gcgccgcttc agcagggcga ttgcgccgcc gcccagacct cactcggcgc
     2641 gcccgatcag agcggcaatt ggtccagcct gccggacccc gcgaaggtct tgcttttcca
     2701 agcgggcgtg gaagcctgca cgggtgatct cacaacggcg ggctcgttgc tggcactcga
     2761 ccgcacactg ttcggcgcca caacgacgga ggatccccaa gacgccgact cagcgtgtgc
     2821 gctctacaag gcgctggtca gcgtgctcga ccaggtagat ccggccagcg cgcaatgtcc
     2881 gaccggtgca gagccggcct ggcagttcga cgcgaacagc aatccgctcg acccgcgtac
     2941 acatcaggac gcgtacgcgc acccggcaac gtcgtccact ccgacgccgg ccccttcggc
     3001 ttccaccgca tcgcccacgg cccacccgtc gcacatcctc gtgcatccgt cgctcatcct
     3061 cgggcactcg ctcgtgacac cgtctgcgac gagttgagcc aacggtgcct gagcaggagc
     3121 ggcccgcgac ggagagtgtc gacgtttccg acgcggccat ggcggggcac gaggtaaccg
     3181 ccagccctgc ggcggcgcct ccggctggtg agacttcagg cggcgctttc gtcggacttc
     3241 ggccgctcgt cgcgttgctc ggcaacgtca cggtgctgac gtcgctgttg tacttcttcg
     3301 gctggcggca gaccgcgacg caggactccg atctgcggct caaccaagac attttcggcc
     3361 tcagtactca ggattacctg cttcggtcgg tcgggtcggt catcaggttg ctgtcggtgc
     3421 tcggagtcgt tggtttgctg ttcgtgtatc tcgatcacgc gttgcgttgg gcggttgcgc
     3481 attacggtcc gcgcgatcgg atcgtgctga tcgcggtgcg ctcactcgac gtcgcatggt
     3541 tggtggtgcc ggtcgtcgtc tacctgttgc ggctgtggcg ccccgacagc tctgacctct
     3601 acatcgccta cccgctcgcg ctcggcgccg gcgtgatgct gatcgtctac cgcgcgcaag
     3661 tgcgtcgcag gcttggcttg cgcaccgatc cgactgatgg ccggtcgcta cttgaacgtg
     3721 gtgcggtggc tttgttattg gtggtcgccg ttttctgggc gacgtccaat tacgcggacg
     3781 tagaggggca ccggctcgcc gaagggtttc gcacaagtga cctgatccca gtgacggtct
     3841 tcagcccgca gcgcatcgat ttgagcggcg cgcaagagca gccaatctct gcgtcctcaa
     3901 cagaccctgc tggcgcgctc tatcaatatc gctatacagg cctgcgcttg atggcccatt
     3961 ctggcggcca ctacttcctc gtccctgcaa gttggacgcc gaccagtggg gctctcgtcg
     4021 ttctctcgga caaggatccg gtgcggttgg agttcggcag ctgatcgccg tccgacgatg
     4081 agtttcggcg ccttggtcag tcaaccattt gcaacgtgcg gacgttgtgt cgtgacgccc
     4141 cggtggtgcg tgcagctggc ctggtcggaa aggatggagc ggtgtcggtg atcgaggtga
     4201 ccggtcttcg caaggtgtac ggcgagcgca ccgtggtcga tggtgtcagc ttctcggtcg
     4261 agccaggcga gatcttcggg attttggggc ctaacggcgc cggaaagacg acgacggtgg
     4321 agtgcatcgg tggcctacgg caccgcgacg gtggctctat caccgtcgcg ggcatggatc
     4381 cggccaagga gggctcggcg tttcggcagg ttctgggaat ccagctgcag gagtcgcggc
     4441 tgccggagaa gatgaccgtg atcgaggccc tggacttgta tgccagcttc tatccttcgc
     4501 ccgccgatcc ggccgagctg ctggatcggc tgggcatggt tgagcagcga aagacctatt
     4561 tcggcaagct gtccggcggt cagaagcaac ggctgtcggt cgcgttggcg ttgatcggca
     4621 acccgagggt ggcgatcctc gacgagctca cgaccggtct ggatccggct gcacgccgcg
     4681 atgtgtggga ccttctggaa gatctgcgcg ctggtggcgt cacgctgcta ttggtctcgc
     4741 acttcatgga ggaggccgag cggctctgcg atcgcgtcat cgtcatcgac cacggaaaga
     4801 tcatcgccgc tgacacacca gaggggctgg cggcaagtgt gcaggccgac cagcgaatga
     4861 gtttcctgcc gtcgacgccg ttcgacctcg ccgtgcttcg ggcgctgccg gatgtgcatt
     4921 cggtcaacac cgtcggcgag cgggtggtgg tgacgggaac cgacaacgtc gccagtgcgg
     4981 tcattctcgg tctgcatggg cagggcatct gggtgggcaa gctacgtgtc gaccagccca
     5041 gcctcgacga cgcgttcgtg gcattgacca ccagcaacca ggcgccggag aactcgagca
     5101 cggaggcgag tcgatgagcg cagccagttc gacgaagccc gtcaccactg cccggatgat
     5161 cccggcgatc aattggcgcg cggcccgcgc gatgctcgtc agcgagggcc ggctgctgct
     5221 gcgaaacccc ggcgtcgtga tctggacagc cgttctgccg gtcgccgccg cgatcatcct
     5281 cgggtcgatc cctgcggtac gcaagccgtc agcgagtttc ggggggctga gcttcttcca
     5341 ggtctatcag ccgatcctgg tgttcttcgc gctcacgttg ttggccatgc aggcgctgcc
     5401 ggatgtgctg actcgctacc gcgaaatggg tgtgctcaag cggcttcgca cgacaccggt
     5461 atcgcccgcg ctgctgctgt gcgcgcagtt gacgctcatc ttgtcggtcg cgatcatctg
     5521 cggggcactg atggtggtcg ccccgccgct gggtggcgct ccctggccgc gcaacatcgg
     5581 tggcttcgtc atctcctacc tgctcggggc gtgggcgatg ctgggcctgg gaatggtgat
     5641 cgccagcctg ttccacaacg ccaaggtcgc cgccggcttc ggatcgttcc tgttcttcgt
     5701 cctgcagttc ttcgccgggc tgtggtggca gcggcccaac atgccgacgt ggctgcgtga
     5761 catctccaac ttcaccccca gcggcgccgc ggtgcaggcg ttgaccaact ccagcgtcgg
     5821 acattggccc ggtgcgctat acctgatcgt cttggcagcg tggggcacgg tgacctcgtg
     5881 gctagcggtc cgcttcttca gctgggaata ggtcgcgggt accggatacc ggcttgtctg
     5941 ggcaagtcag gaccgttggg cggcatactg cggcggtgcg actgacagat ttctggtggc
     6001 ggatgcggca gcatttcggc ccgacgtatg ccgagtcgtt cgcccacgac ttcgtgcttg
     6061 gcgagcttgg tggccgcacg attgatgagg cgctggccca gggcgtcgac gtccaggaga
     6121 tctggcgggc ggtctgccga accgtcgagg tggccccgaa actgcgctga actgtggcgt
     6181 ttgtgactga tggtggctgt gggttcccag tggttttcag cggcctccgg ggcgtacttc
     6241 tagtcggcgt gtcgccagtc attcgaacag gtgttcggtt actgtccaca gctatcgaag
     6301 tggtccacag cggtggcccg gcggcgggaa agtgacagac ctgccgccta acgtcgtcca
     6361 cgacccggag atttgagacc caacggcgac tgaacgggat ggcgacacca tggcagcacc
     6421 tgaccgcgcg aaggccctcg agaacgcgct tgcccagatc gagcgcgctc acggcaaggg
     6481 ttcggtgatg cgcctgggtg atgaggtgcg agagccgatc gaagtgatcc cgaccggctc
     6541 gatcgcgctt gacgtcgcgc tcggcatcgg cgggctgccg cgtggtcgga tcgtggaaat
     6601 ctacgggcct gagtccagcg gcaagacgac gctggcgttg cacgcggtcg ccaacgccca
     6661 gcgggctggc ggcattgcgg ccttcatcga cgccgagcac gcccttgacc ctgagtacgc
     6721 cgccaagctc ggcgtcgaca gcgacgcgtt gctggtgtcg cagcccgaca ccggcgagca
     6781 ggcactcgag atcgctgaca ctttgatccg ctcgggtgct atcgacatca tcgtcatcga
     6841 ctcagtcgct gctttggtgc cccgcgccga gatcgaaggc gagatgggcg acagtcacgt
     6901 cggcttgcaa gcacggttga tgagccaggc attgcggaag atcaccggtg cgttgagcaa
     6961 ctccggcaca acagcgattt tcatcaacca gctgcgcgag aagattggcg tcttcttcgg
     7021 gtgcttcaac tacaacaccc gcgtcaccct tgcagacggc acgcaggaga agatcggcaa
     7081 gatcgtcaac cagaagatgg cggtagaggt tctctcctac gatcccgaga ctgacaggat
     7141 cgttcctcgc aagatcgtca actggttcga caacggtccg gctgacgaat ttctccagtt
     7201 caccgtcgcg aagtctggca agaacgggcg cgcgcagttc gctgccactg ccaaccacct
     7261 catccggacg ccgggtggct ggcgtccggc cggcgagttt ctcgctggtg accgggtgat
     7321 ggtcactgag ccacatcggt tgagctcaca gcagatgcag gtcgtgctcg gatcgctgat
     7381 gggcgacggc aatctgtcac cgaaccgcag gggtcgtagc ggtgtgagat tc
//
LOCUS       DASWVF010000339         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_314648, whole genome shotgun sequence.
ACCESSION   DASWVF010000339 DASWVF010000000
VERSION     DASWVF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314648"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VGD55_06090"
     CDS             complement(<1..1589)
                     /locus_tag="VGD55_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0869946.1"
                     /translation="MTTSQIVAGGDVAPTPEQIAQAFPVLTEDQRGRLRTYGVAEVVN
                     VGDTLFVPGDLTYDLVLIEQGVVDIVSTPTDGTPEQIVVQHKAGAFLGELNLLTGQIV
                     YLTARVAEAGTVYRVSGESFRRVMAEDPELSDLLLKAFLARREFLRFSPAARNIEIVG
                     SSLSAQALALRTYAARQRLPHLWFDSEAVDGTALMSAAGLTLADLPAVLTPGRVLRSA
                     TPGELAEHLGLSYHRAPGKAVDLVVVGGGPAGLAAAVYGASEGLATVLIDAVATGGQA
                     AASSRIENYLGFPSGLSGAELTGRASIQALKFGAELSSPCKAASLDCSTGQLRVVLVD
                     GTDIVTRSVILATGARYRALPLPRWPDFEGAGIYYAATELEARLCGNQPVAVIGGANS
                     AGQAALYLASRESSVSLVVRGDDLFAGMSSYLADRLLASPQVTIHTQAEVTALHGDAS
                     LSNITVTNRVTGETHDAACKGLFCFIGAVPATDWLTGIALDEDGFIRTDLQLVDADLR
                     EIWTDLGRRPLPFETNVPAVFA"
     gene            complement(1645..2475)
                     /locus_tag="VGD55_06095"
     CDS             complement(1645..2475)
                     /locus_tag="VGD55_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869947.1"
                     /translation="MSVVTAANPSTSPHKPFVFGRLLLAEWTKLRTVRSTMWSLVLLV
                     VVVPGLTFLLTLLISAQWSKSDAGSRAQIVADPTSTILGTGFQLGQLTICVLGVMVIA
                     SEYSTGMIRASLLAVPKRTPMLAAKGVVFAALVFVFGEIVAFPSFFIGAAILHKHAPV
                     SIGDPGVLRAVIGAGLYLTVLGLFALAVAAIVRHTAGAITGVIGFVLVLFPLTKLLPG
                     TVGKHVSAYMPSLAGQLIAHAHQAKDDLLSPWQGFGVFCVWTALLLAVAAYQLRRRDA
                     "
     gene            complement(2475..>3035)
                     /locus_tag="VGD55_06100"
     CDS             complement(2475..>3035)
                     /locus_tag="VGD55_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0869948.1"
                     /translation="GFSLGMGQRLGLAAALLGDPQVLMLDEPVNGLDPEGIVWIRNLL
                     KYLASQGRTVLVSSHLMNEMAVTADHLIVIGRGRLVADCTTQEFIEGHSEHTVLVKSP
                     DLAKLREVVIAAGARVTNDADGEFTVRDLPAPRIGELAARDGLVLYELATQHASLEEA
                     FMELTHESAEYAAPSVPPSDAAGGAA"
BASE COUNT          578 a         1016 c          978 g          463 t
ORIGIN      
        1 gcgaaaacgg cgggcacgtt ggtctcgaac ggcaatggcc gccgtccaag atcagtccag
       61 atctctcgca gatctgcgtc gaccaactgg agatcggtgc ggatgaagcc gtcctcgtcg
      121 agcgcgatac cggtcaacca atcggtggcc ggcaccgcac cgatgaagca gaacagtcct
      181 ttgcaggccg cgtcgtgcgt ttcaccggtc acccgattgg tgacggtgat gttgctcaag
      241 gacgcatcac cgtgcagcgc cgtcacttcg gcctgcgtgt ggatcgtgac ctgcggcgag
      301 gcgagcagac gatcggcgag gtaggacgac atgccggcga acaggtcgtc gccgcgcacc
      361 accagagaca ccgagctttc gcgcgacgcc agataaagtg cagcctggcc cgctgagttc
      421 gcaccgccga tgaccgccac gggctggttg ccgcataggc gcgcctcgag ttcggtcgcc
      481 gcgtagtaga tgccggcgcc ttcgaagtcg ggccagcgcg gcaacggcaa tgcccgatag
      541 cgagcgcccg tcgcgagaat caccgatcgc gtgacgatgt cagtgccatc tacgaggacg
      601 acgcgcagtt gccctgtact gcaatcgagc gacgcggctt tgcacgggct cgaaagctcc
      661 gcaccgaact tcagcgcctg gatcgacgcc cggccggtca gttcggcccc gctcagcccc
      721 gaagggaagc cgagataatt ctcgatccgc gaactggcgg ccgcctgacc accagtcgcg
      781 accgcgtcga tcaggaccgt ggccaacccc tctgacgcgc cgtagacagc agcggcgaga
      841 ccagcaggac caccaccgac caccacgagg tcgacggcct tgccaggcgc gcggtgatac
      901 gacaaaccga ggtgctcggc gagttcgcct ggtgtggcgc tgcgcaacac ccgacccggt
      961 gtgaggaccg ccggcaagtc ggcgagcgtc agccccgctg ccgacatgag cgccgtaccg
     1021 tcaacggctt cagagtcgaa ccacaggtgc ggtagccgtt gccgcgcggc gtacgtgcgc
     1081 aacgcgagcg cctgcgccga caagctgctg ccgacgatct cgatgttgcg cgccgccgga
     1141 ctgaagcgca ggaactcccg ccgcgcaagg aacgccttca gcagcaggtc agaaagctcc
     1201 ggatcttccg ccatcacccg gcgaaaagac tcccccgaaa cccggtacac cgtgcccgcc
     1261 tctgccacgc gagccgtcag gtagacgatc tgcccggtca gcaggttgag ctcaccgaga
     1321 aacgcaccgg ccttgtgctg gacgacgatt tgctcgggcg tcccgtcagt cggcgtactc
     1381 acgatgtcga cgacgccctg ctcgatcagc accaggtcgt aggtgagatc gcccgggacg
     1441 aaaagtgtgt cgccgacgtt cacgacttca gccacgccat acgttcggag ccgcccgcgc
     1501 tgatcctccg tgaggaccgg gaacgcttgc gcgatttgct ccggggtcgg cgccacgtcg
     1561 ccgcccgcca cgatctggct tgttgtcatg gtcacgtgga gctccttgcg aggttgtagc
     1621 cgctcagagc acgatacgtg ctcgtcaggc gtcgcgacgt cgcagctggt acgcggcgac
     1681 agcgagcagc agcgcggtcc aaacgcagaa gacaccgaac ccctgccacg gactcagcag
     1741 gtcgtccttg gcctggtggg catgcgcgat cagctggccg gccagcgacg gcatgtacgc
     1801 gctgacgtgc ttgccgacgg ttcctggcaa caatttggtg agcggaaaca gcacgagcac
     1861 gaatccgatc acgcccgtaa tcgcaccggc ggtgtgacgg acgatcgctg cgacggcgag
     1921 cgcaaacagg ccgagcacgg tcaggtagag gcccgctccg atcaccgcgc gcagcacgcc
     1981 cgggtcaccg atcgagaccg gtgcgtgctt gtgcaggatc gccgccccga tgaagaacga
     2041 agggaacgcg acgatctctc cgaagacgaa caccagcgca gcaaagacga cccctttggc
     2101 ggccagcatc ggcgtgcgct tgggcaccgc gagcaggctg gctcggatca tgccggtcga
     2161 gtactcactc gcgatgacca tcacgccgag cacgcagata gtgagctggc ccagctggaa
     2221 acccgtgccg agaatcgtgc tcgtcggatc ggcgacgatc tgcgcccgcg acccggcgtc
     2281 ggacttgctc cattgcgccg agatcagcaa cgtcagcagg aaagtgagcc caggcacgac
     2341 aacgacgagc agcacgagcg accacatcgt cgaccgcacc gtgcgaagtt tggtccattc
     2401 cgcgagcaac agtcgcccaa agacgaaggg cttgtgaggg gacgtcgagg ggttggccgc
     2461 cgttacgaca ctcatcacgc cgcacctcca gccgcatcgc tcggtggcac gctcggtgcg
     2521 gcgtattccg cgctctcgtg ggtgagttcc atgaacgcct cctcgagcga cgcgtgctgg
     2581 gtggccagct cgtagagcac taatccatcg cgcgcggcca actctcctat tcgcggggcg
     2641 ggcaggtcgc gcacggtgaa ctccccgtcg gcgtcgttag tcactcgcgc gccggcggca
     2701 atgaccacct cccgcagctt tgcgaggtcg ggtgacttga cgagcacggt gtgttctgag
     2761 tgcccctcga tgaactcctg cgtcgtgcaa tcggcgacga gccgaccgcg accgatcacg
     2821 atgaggtggt cggcggttac tgccatctcg ttcatgaggt ggctcgacac caacacggtg
     2881 cgcccctgcg aagccaggta cttgagcagg ttcctgatcc acacgatccc ttcaggatca
     2941 aggccgttga cgggctcgtc gagcatgagc acctgcgggt cgccgagcag cgcagcggcc
     3001 agaccaaggc gctggcccat gccaagggag aagcc
//
LOCUS       DASWVF010000340         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_467912, whole genome shotgun sequence.
ACCESSION   DASWVF010000340 DASWVF010000000
VERSION     DASWVF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467912"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGD55_06105"
     CDS             complement(<1..263)
                     /locus_tag="VGD55_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY0869949.1"
                     /translation="MFLGTHAPRLDAKGRLFLPAKFRDELAEGLVITKGQERCLYVFP
                     AVEFGRLTEALRTSPVTHKGVRDYSRVFFASASDDIPDKQGRIT"
     gene            626..1624
                     /locus_tag="VGD55_06110"
     CDS             626..1624
                     /locus_tag="VGD55_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0869950.1"
                     /translation="MAAFPEAEVGDLKEVAETGRRICAAVEQVIEGKPDVVQLAVTVL
                     LAEGHLLIEDVPGVGKTMLAKALAKAIDCSVRRIQFTPDLLPSDITGVSVFSQDTREF
                     EFKPGPIFANIVVGDEINRASPKTQSALLESMEERQATVDGQTYRLTAPFMVVATQNP
                     IEMEGTYPLPEAQRDRFTARLSMGYPSAAAELEMLDSHGTREPLDDLVPVADAAEVAK
                     AIELVRTVYVAPDVRRYTVDLVAATRASSELRLGASPRATLQLLRTARAAAALDDRDY
                     VLPDDVRALTQPVLTHRLLINAEAQISRRTASEILADIVSRVAVPSPSPRSRANPR"
     gene            1708..2919
                     /locus_tag="VGD55_06115"
     CDS             1708..2919
                     /locus_tag="VGD55_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0869951.1"
                     /translation="MSETDLLRVGIFLCALPIATAVVVARTRYRLSCVRRIEPRRVPV
                     GESAEVVLRVENLSRVATGILLLEDRLPYSLGGRPRFVVNRIESRGTREVSYPVRSDV
                     RGRFVVGPLSLRFADLFGLVELTRSFTTTDALVVTPTVTPLPEIRLGGDWAGGGDSRA
                     RSISTMGEDDVTTREYRHGDDLRRVHWRSTARYGELMVRREEQPWQNRGVLLLDSRVA
                     AHRGEGPGSSFEWAVSAIASVGCHLARDGFGLRLVTDVGTEVSGYSADAMAGTPFESV
                     LLDSLALVTMSAGHSFGPGLAAIRRGGGEGLVIAALGSLTDADLDALARFRHHASACV
                     AIMLDTSTWSSTPQVGSPHSSGARERSHAQSCALLSGAGWRVVSVTARTDMRTMWGNA
                     ATNSRALAGRS"
BASE COUNT          520 a         1045 c          985 g          535 t
ORIGIN      
        1 gtgatccggc cctgcttatc ggggatgtcg tcggatgcgc tcgcaaagaa gacccgcgag
       61 tagtcccgta cgcctttgtg cgtcaccggc gaggtgcgaa gggcctcggt caatcggccg
      121 aactccacgg ccggaaaaac atagaggcac cgctcctggc ccttggtgat caccagaccc
      181 tccgcgagct cgtcgcggaa cttcgctggc aggaacagtc tgcccttcgc gtcgagccgc
      241 ggggcgtgcg ttccgaggaa caccagccac ctgcactcgc gatctgttca gtacctcggc
      301 tctccattcc gcgccactgt actccacact cactcacttt caagtagttc gagcccatga
      361 tccgggccgg ctgctccctg atgtctcagg gcgaccgagg aacacatggg gaacagacca
      421 ggagcggacc ggcgaaccga tcggcgtccg tgcacgttcg caacaagtga cgcaggccga
      481 gaacggacaa ccagctactg ggatcactcg tttgcacgct cgtgacctga gagactcatg
      541 ggcaagctca agccagaacc tcaagcacga cgtggcaccc caagccaggc cgggcacgga
      601 caagcaccaa cagcgaggag gacgggtggc tgcgttcccg gaagcagagg tgggcgacct
      661 caaagaggtc gcggaaacag gtcggcgcat ctgcgccgcg gtggagcagg tgatcgaggg
      721 gaaaccggac gtcgtccagc tggccgtcac cgtgctgctc gccgaaggcc acttgctcat
      781 cgaggacgtc cccggcgtcg gtaagacgat gcttgccaag gccctcgcca aggccatcga
      841 ctgctccgtg cgccgtatcc agttcactcc ggacctgctg ccgagcgaca tcacgggtgt
      901 ctcggtcttc agccaggaca ctcgagagtt cgagttcaaa cccgggccga tcttcgcgaa
      961 catcgtggtc ggcgacgaga tcaaccgcgc atcgccgaag acccagtccg cgctcctcga
     1021 gagcatggag gaacggcagg cgaccgtcga cggacagaca taccgactga cggccccgtt
     1081 catggtcgtt gcgacgcaga acccgatcga aatggaaggc acttacccgc tgcccgaagc
     1141 gcagcgcgac cgcttcaccg cgcggctgtc gatgggatac ccgtcagcgg ccgccgaact
     1201 cgaaatgctc gattcgcacg gcacccgtga acctctcgac gacctcgtgc ccgtcgccga
     1261 cgcggccgag gtagctaagg cgatcgaact ggttcgaacc gtgtacgtcg cgccagacgt
     1321 caggcgctac acagtcgatc tcgttgctgc gaccagagcc tcgtcggaac ttcgcctcgg
     1381 cgcatctcca cgtgcgacct tgcaactgct gcgtacggcc cgcgccgccg ccgcacttga
     1441 cgaccgcgac tacgtgctac cagacgacgt ccgcgcattg actcagccgg tgttgactca
     1501 ccggctgctt atcaatgcag aggcgcagat ctcgcggcgc accgcgtcgg agatcctcgc
     1561 cgacatcgtc agcagagtcg cggtcccgtc gccgtcaccg cgctcgcggg ccaacccgcg
     1621 ctgatgcggg ctgcgctctc aggcctcacg acccgcggcc gaagctttct cgccgcggga
     1681 gtagcggcgt gcctgtgcgg attcctgttg agcgagaccg acttgttacg tgtcggaatc
     1741 ttcttgtgtg cgttgccaat tgcgacagct gtcgtcgtcg cacgcacgcg ttaccgcctg
     1801 tcctgcgtgc gacgcataga accgcgacga gtacccgtgg gcgaaagcgc cgaggtggtg
     1861 ctgcgggtcg agaacctctc gcgcgtcgcg accggcatcc tgctgctcga agaccgtttg
     1921 ccctacagcc ttggcggcag gccgcggttc gttgtgaacc gcatcgaatc gcgcggcaca
     1981 cgtgaggttt cctacccggt tcgaagcgat gtccgcggcc gcttcgtcgt cggcccgttg
     2041 tcgttgcgtt tcgccgacct cttcggactc gtcgagttga cgcgctcgtt caccacgaca
     2101 gatgcactcg tcgtgacacc gacggtgacg ccgttgccag agattcggct cggcggcgat
     2161 tgggcgggcg gcggcgacag ccgcgcacgt tcgatatcaa ccatgggtga agacgacgtc
     2221 acgacccgcg aataccgtca cggcgacgac ctgcgccgcg tgcattggcg atcgactgcg
     2281 cggtatggcg agctgatggt gcgacgcgag gaacagccgt ggcaaaaccg tggggtgctg
     2341 ctgctcgaca gccgggtggc cgcacaccgc ggcgaagggc cgggttcgtc gttcgagtgg
     2401 gccgtgagcg cgatcgcatc ggtgggctgt cacctcgcgc gcgacggctt cggtcttcga
     2461 ctcgtcaccg acgtcggcac cgaggtctct ggatattcgg ccgacgcgat ggccggcacc
     2521 ccgtttgaat cggtgctcct cgacagtctc gcgctggtca ccatgtcagc tggacactcg
     2581 tttggtcccg ggctcgcagc aatccggcgg ggcggcggcg aaggcttggt gatcgcggcg
     2641 ctcgggagct tgaccgacgc cgacctcgac gcgctcgcgc gattccgcca tcacgcatcc
     2701 gcctgcgtgg cgatcatgct cgacacctcc acgtggtctt cgaccccgca ggttgggtcc
     2761 ccacattcgt cgggtgcccg cgagcgcagc cacgcgcagt cgtgcgcgct tctctcgggc
     2821 gccggctggc gagtcgtctc cgtcactgcg cgcaccgaca tgcgaacgat gtggggaaac
     2881 gcggctacga actcgcgcgc actagcgggc cgctcatgaa cccgcggctt cgtatcaccg
     2941 tgttcgctgg gcttgccacg atcctgggct cgctgagcct gctgccgatc ttcagctcga
     3001 ccggctggct gccgcgcgtc ttgctgatgg tcggcgcgat ctccgcaacc tcggccttgc
     3061 tgcaacgcgt acgcgccctg cgtgc
//
LOCUS       DASWVF010000341         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_416966, whole genome shotgun sequence.
ACCESSION   DASWVF010000341 DASWVF010000000
VERSION     DASWVF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416966"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGD55_06120"
     CDS             <1..902
                     /locus_tag="VGD55_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0869952.1"
                     /translation="GPVTQETLTTIDGRVLCYGQWGDPAGFPVFSLHGTPGCRLNRYP
                     DDDAVAAVGARLITYDRPGYGRSDRHRGRQVVDCVGDVAAIADALGIERFAVTGGSGG
                     GPHALAVGARLPDRVTIARSVVGVAPVDAADLDWSAGMDAENVKEFGWALAGEATLYE
                     ELTKAAAEMVANVKADASKVLGDQWQLAEADRAAMRNPIVMQVMREAIPECVVNGPWG
                     WVDDDLAFTNAWGFDLDEIRVPVEVHYGSQDVLVPAAHGAWLALNVPGAKVVVNDDQG
                     HMNTPEHTLELLRTLVYDSTHQA"
     gene            complement(899..2263)
                     /locus_tag="VGD55_06125"
     CDS             complement(899..2263)
                     /locus_tag="VGD55_06125"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEY0869953.1"
                     /translation="MDVHDSLLDLVGETPLVCLSRVATPGGAQLLAKLEQLNPGGSVK
                     DRIAVRMVETAEASGALKPGGTIVEPTSGNTGVGLAIVAARKGYRCVFVMPDKMSAEK
                     INVLKGYGAQVVVCPTAVAPEDPESYYSVSDRLVREIEGAWKPDQYSNPDNPRSHYET
                     TGPELWRQTDGLITCFVAGIGTGGTISGTGRYLKEVSGGAVRVVGADPEGSVYSGGSG
                     RPYLVEGVGEDFWPGTYDRTICDDIIAVSDKDSFLMTRRLARDEGILVGGSSGMAVVA
                     AVHVASHLSPDDVVVVLLPDSGRGYLSKIFNDEWMADYGFLAGSGDTTVADVLERKGT
                     RLPEMVHLHPTETVREAIDVLREYGVSQVPVVRAEPPVMSAEVVGSVVERDLLDALFS
                     GRAALSDALEKHISAPLPMIGGGEPVSAAMIALETADAAVVLIDGKPAGILTRQDLLG
                     FLAH"
     gene            complement(2566..3531)
                     /locus_tag="VGD55_06130"
     CDS             complement(2566..3531)
                     /locus_tag="VGD55_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0869954.1"
                     /translation="MNTASSSAPASSTPGFTSTTASNAGERALVLGGGGSAGNAWLIG
                     VIAGLFEAGLDVTQADLIIGTSAGATAAAQVTSATTPTELLAAILATGPQPQPGPVGP
                     DGGRVRIGPAANHMETTSAIIAAAEDAADMRRRMGAAALEIDAASDGSGQTQWRATVA
                     ARLSSQLWPDRTMLITAVDARSGEPVVFDRHSGVELVDAVAASTANGFGVPPYRIGDN
                     RYIDGGYRSNADNADLAAGYARVLVLAPLGGRSRTPPAWGMQLATQVHDLRERGSRVE
                     TIFPDNNSRTAFGDNMMDLSTRPPAARAGYDQGRALAEPLTAVWL"
     gene            complement(3772..4155)
                     /locus_tag="VGD55_06135"
     CDS             complement(3772..4155)
                     /locus_tag="VGD55_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869955.1"
                     /translation="MVAVPASFAEDLARVVTTLRTTPAGRLRARLAGPFSSRAEAGRA
                     LAGVLANAAQGIEDADQPAMPQWRTPPVLADLAVGDQVRVLGHDFLSALPGAAKVVWT
                     PAGRVSRDELVRDVVASLAEVAAFL"
BASE COUNT          782 a         1448 c         1373 g          699 t
ORIGIN      
        1 gaggcccagt gacacaagaa acgctaacga ccatcgacgg acgggtgctg tgctacgggc
       61 agtggggtga tccggcgggg ttcccggtct ttagcctgca tggcacgccg ggttgtcgtc
      121 ttaatcgcta tccggacgac gatgcggtgg ccgcagtcgg cgcgcggctc atcacctatg
      181 acaggcccgg ctacgggcgc tccgatcgcc atcgtggtcg ccaggtcgtc gattgcgtcg
      241 gcgatgtcgc agcgatagcc gatgcgctcg gcatcgaacg gttcgccgtg acgggcggtt
      301 cgggcggcgg cccacacgca ctcgcggtcg gagcacgctt gcccgatcgc gtcaccatcg
      361 ctcggagcgt ggtcggagtc gctccggtcg acgcggccga cctcgactgg tctgccggca
      421 tggatgcaga gaacgtgaag gagttcgggt gggcattggc tggcgaagcc acgctctacg
      481 aggagctaac caaagcggct gccgaaatgg tcgccaacgt caaggccgat gcgtcgaagg
      541 tacttggcga ccaatggcaa ctcgccgagg ccgaccgagc ggcgatgcga aacccgatcg
      601 tgatgcaggt catgcgcgag gcgataccgg agtgcgtcgt caacggcccg tggggctggg
      661 ttgacgacga cctagcgttc accaatgcgt ggggcttcga tctcgacgag atcagggttc
      721 cagtcgaggt gcactacggc agccaggacg tccttgtgcc ggccgcacac ggcgcctggc
      781 tggcgttgaa cgtgccaggt gcaaaggtcg tcgtcaacga cgaccagggg cacatgaaca
      841 cgcccgaaca cacgttggag ttgctgcgga cacttgtcta cgacagcacg caccaggctt
      901 agtgagcaag aaaaccgagc aaatcctggc gggtgagaat gcctgccggt ttcccgtcga
      961 tcaacacgac cgcagcatca gccgtctcga gagcgatcat cgcggccgac acgggttcgc
     1021 cgccgccgat catcggcagc ggggccgaga tgtgcttctc cagtgcatca gacaatgccg
     1081 cacgaccaga gaacagtgca tcgagcaggt cgcgttcaac gacggagccc accacttcgg
     1141 ccgacatcac cggcggctct gcgcgaacca ccggcacctg cgagacgccg tactcgcgca
     1201 acacgtcgat cgcctcgcgc actgtctccg tcgggtgcag gtggaccatc tcaggaagcc
     1261 gagttccctt gcgctccaag acatctgcga cggtcgtatc gcccgaccct gccaagaatc
     1321 cgtagtcggc catccactcg tcattgaaga ttttcgacaa gtagccacgc ccggagtctg
     1381 gcaacaacac caccaccacg tcgtccggcg aaaggtgcga agcgacgtga acggccgcga
     1441 ctaccgccat gccactcgac ccaccgacga ggatgccctc atcgcgggcg agccggcgcg
     1501 tcatcaggaa cgagtcctta tcggagaccg caatgatgtc gtcgcagatc gtgcggtcgt
     1561 aggtgcccgg ccagaaatcc tctccgacgc cctcgacgag gtagggccgg ccgctgccgc
     1621 ctgagtagac ggacccttcg ggatcggccc cgaccacgcg aaccgcgcca ccagagactt
     1681 ccttgaggta ccggccggtg ccgctgatcg tgccgcccgt gccgataccc gcgacgaaac
     1741 aggtgatcaa gccgtcggtc tgccgccaca gttcgggccc ggtggtctca tagtgcgagc
     1801 gcgggttgtc ggggttgctg tactggtcgg gcttccacgc gccttcgatc tcgcgcacca
     1861 gccgatctga caccgagtag tacgactccg gatcctcagg cgcgaccgcc gtcgggcaga
     1921 caacaacttg cgctccatag cccttgagaa cgttgatctt ttcggcactc atcttgtcgg
     1981 gcatcacgaa cacgcagcga tagcccttgc gcgcggcgac gatcgcaaga ccaacgccgg
     2041 tgttgcccga cgtcggttcg acaatcgtgc cgccaggttt cagcgcacct gaagcctcgg
     2101 ccgtctcgac catgcgaacc gcgatccggt ccttgacgga accacccgga ttcagctgct
     2161 cgagcttggc cagcagttgc gcgccaccag gcgtcgcgac gcgcgacaga caaacgagcg
     2221 gcgtctcccc gacgaggtcg agcaagctgt catgaacgtc cacaagtccg agcctagtca
     2281 ggccggacgc cggatcgagc gacgcgtgac accagtttgt aaagccatgc aagttcgccg
     2341 ccgtgttcag ctcatgccca tcctcaccgg gtcgcccgct cggcccaacc tggacacgtg
     2401 accccgctca tccgactcca gccgccgcac atggacggcc gggcggagac gagcgtcaat
     2461 gcggcggatt cggatgctaa ttcccgcgca aagtcgatgg ctacgtaccg caaccgtctc
     2521 aggatccgtc gtcgtaggac tgtcgaggcg tttgcttctt ggatgtcaga gccagactgc
     2581 cgtgagcggc tcggcaaggg ccctgccttg gtcgtatccg gctcgtgcgg cgggtgggcg
     2641 cgtggacagg tccatcatgt tgtccccgaa tgcagtgcgg gagttgttat cgggaaagat
     2701 cgtctcgact ctgctgccgc gttcgcgtag atcgtggacc tgtgttgcca gctgcatccc
     2761 ccatgccggc ggcgtccgtg atctgccacc caatggtgcc agcaccagca cccgcgcgta
     2821 tcccgctgcc aggtcggcgt tgtcggcgtt ggatcggtag ccgccgtcga tgtatcggtt
     2881 gtcaccgatg cggtagggag ggacaccaaa gccgttggca gtgctggccg cgacggcgtc
     2941 cacgagctcg actccgctgt gacggtcgaa gacgaccggt tcacctgaac gggcgtcgac
     3001 cgccgtgatg agcatcgttc gatccggcca aagctgactg gacagccgag ccgcgacggt
     3061 cgcgcgccac tgcgtttgcc cagagccgtc cgacgccgca tctatttcga gcgccgccgc
     3121 gcccatcctg cggcgcatgt cggccgcatc ctcagcggcc gcgatgatcg cactcgttgt
     3181 ctccatgtgg ttcgccgccg gcccaatccg aacacgtcca ccatcaggcc cgaccggacc
     3241 aggctgtggc tgcggcccag tggcgaggat ggcggcaagc agttcggtcg gtgtagtggc
     3301 gctagtaacc tgagccgcgg ccgtggctcc agccgaggtc ccgataatca ggtcagcctg
     3361 ggtcacatcc aacccggcct cgaacagacc ggcgatgacg ccgatcagcc acgcattgcc
     3421 ggccgatccg ccaccgccaa ggaccaacgc tcgctcgccc gcgttggagg cggtcgtcga
     3481 ggtgaagccc ggggttgaag aagccggagc tgaagaagat gccgtattca tgggagtcgc
     3541 ctttcgtgaa ctgcctgttg ggcgctccca gtggcgacta cgtcagtcgc gcaatcgtgc
     3601 accgcagggg agcacccact accgataccg cgttcatggg tttcacctcc tatcgatgga
     3661 taacgaccgc gaaaacgcta gcacgccggc aacgcggccg tggcgctgtc gaacacgtcg
     3721 ccgacagcca cgcccccata cacctgttgc cgtagaggtg atcggaggtg ctcagaggaa
     3781 agcggcgacc tcggcgaggc tcgccaccac gtcgcgcacg agctcgtcgc gcgaaacacg
     3841 ccccgcagga gtccacacga ctttggccgc gcctggcagc gccgacaaga aatcgtgtcc
     3901 gagcacccgc acctggtcac ccactgcgag atcggccaga accggtggcg tgcgccactg
     3961 cggcatcgcc ggctgatcgg cgtcctcgat accttgcgcg gcgttagcca gaactccggc
     4021 gagcgcccgg cctgcctcag cccggctgct gaacggcccg gccagccggg cgcgcaaccg
     4081 gcccgccggg gtcgtgcgca gggtcgtcac cacccgagcg agatcctcgg caaacgacgc
     4141 agggacggcg accacggccc gcaccgtatc gcgcccagct cgttcgcggc gcgttcgccg
     4201 aaagacttgc cccggcgggc ctcgtgggag caccctgaac tcatggaccg ctaagagttc
     4261 ggccgctcat ggaggcgctc atgaccgtgc accactccga gg
//
LOCUS       DASWVF010000342         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_111118, whole genome shotgun sequence.
ACCESSION   DASWVF010000342 DASWVF010000000
VERSION     DASWVF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111118"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGD55_06140"
     CDS             complement(<1..889)
                     /locus_tag="VGD55_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0869956.1"
                     /translation="MTTSVGSVKRLFVGRPLRSQQLHETLLPKWLALPIFASDPISSV
                     AYATEEIVLVLAVGGGAAALTYAKNVAAAVALLLVIVTLSYRQICKAYPNGGGAFAVS
                     LDNFGENAALTAGSALLVDYVMTVAVSVVSGVVAITSAFPSLHRHAVALSVGFVVLLM
                     VANLRGVKESGRAFAVPTYSFIGLTIVMFAVAAVKAANGGLPRASTASETFTHSHLAG
                     LALILLTLKAFASGCTALTGVEAISNGVPSFRKPKARNAAKTLTAMGTLAVSMFIGIT
                     VLALHVKAHAQPSGNPSVIS"
     gene            1031..2167
                     /locus_tag="VGD55_06145"
     CDS             1031..2167
                     /locus_tag="VGD55_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0869957.1"
                     /translation="MTWRRSLRAASGIGGRRLLGGFALEAVGLPVLTLCLVAVRSHLV
                     FADDLLLYLAAVIAVTLVGGFWPSVVAAIASSLLLNWYFTPPVHTWTIDSPQNVLALA
                     LFVAVAVTVSSVVHLAARRAVLAAGLATEAARAQGLAEGNRIRTALLAAVGHDLRTPL
                     ASVKASVSTLRQTDVTWSPQDQSELLATIEEGADRLNGLISNLLDMSRLQTGAVQLFV
                     RPTSLEEVAPLVVQGVDDGGLLQVEMSEDLPLFAADPGLLERALANLVANALRYSPPG
                     QPPTLAARAVQNGNAPSSVQIDVIDHGPGVPAEQWDRIVQPFEQVGDPRSGNGVGLGL
                     AVTKGFVEAMGGKLSAAATPGGGLTMSIELAVITAGSDGGGEQP"
     gene            2164..2844
                     /locus_tag="VGD55_06150"
     CDS             2164..2844
                     /locus_tag="VGD55_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869958.1"
                     /translation="MTSVLVIDDEAAILRALRINLSARDYQVTTASDGASGLAAMARE
                     RPDVVILDLGLPDMAGTDVIRGIRGWSSVPIIVLSANEQELQKVQALDAGADDYVTKP
                     FGMNELLARLRAATRRAAPGVDEPVVVTPEFTIDLGAKRVVRKDGRDVRLTPTEWQLL
                     EVLARHEGRLVSGRQLLQDVWGPTYGTETNYLRVYMAQLRRKLEPDPARPRYLITEPG
                     MGYRLMTD"
BASE COUNT          524 a          994 c         1041 g          498 t
ORIGIN      
        1 gagagatcac cgacgggttg ccgctcggct gagcatgcgc cttgacgtgc agggcaagaa
       61 ccgtgatgcc gatgaacatg ctgaccgcga gcgtgcccat ggcggtcagc gtcttcgcgg
      121 cattgcgtgc ctttggcttt cggaacgacg gcactccgtt gctgatggcc tcgacgccgg
      181 tgagggcagt acatccagac gcgaaggcct tcagtgtcag caagatgagc gcgagcccgg
      241 ccagatggct gtgggtgaac gtctccgacg cggtcgatgc gcggggtaac ccgccgttcg
      301 cagctttgac tgcagcaacg gcgaacatca cgatcgtcaa accgatgaac gagtaggtgg
      361 gtaccgcgaa cgcgcggccg gactccttca cgccgcgcag gttggccacc atgagcaaga
      421 ccacgaagcc gaccgacaac gcgaccgcat gacggtgcaa cgacgggaac gcgctggtga
      481 tggcgacgac accagaaacc acggagaccg cgaccgtcat gacgtagtcg accaggaggg
      541 cgctgccggc agtcagggcg gcgttctcac cgaagttgtc gaggctgacc gcaaaggcgc
      601 ccccaccgtt cggatacgcc ttgcagatct gtcggtacga cagcgtcacg atgaccagga
      661 gcagcgcgac cgcggcagca acgttcttgg cgtaagtcaa ggcggccgcc ccgccgccga
      721 ccgccaggac gaggacgatt tcctcggtcg catacgccac cgacgagatc gggtcggacg
      781 caaagatcgg cagcgccagc catttcggca gcaacgtctc gtggagttgc tggcttcgaa
      841 gtggccgtcc gacgaagagt cgtttcacgc tgccgactga agtggtcact atctcgacgc
      901 tagtagggct cgaccgacct gagcgtcagt gcgacgtcaa gatcgatcgg gcaaccgtca
      961 aggaagcgtc aagatcgtgg cgcgcccggc cggcggggtg atctggtcgt aggagggcca
     1021 cactcgtgtc gtgacgtgga gacgatcgct gcgcgccgct tcaggcatcg gcggccgtcg
     1081 gctcctcggc ggattcgcgc tcgaggcggt aggccttccc gtccttaccc tttgtctggt
     1141 ggccgtgcga tctcacctgg tgtttgctga cgacctgctg ctctatctgg ctgccgtgat
     1201 cgccgtcacc ttggtgggcg gcttctggcc gtcggtggtc gcagccatcg ccagcagcct
     1261 gttgttgaac tggtatttca ccccgccggt gcacacctgg acgatcgatt cgccgcagaa
     1321 cgtgctcgct ctggcgctgt tcgtcgcagt tgccgtcacc gtgagcagcg tcgtgcatct
     1381 ggccgctcgc cgcgccgtgc tggccgccgg gctggcgacg gaagccgccc gcgcgcaggg
     1441 gttggcggag ggcaaccgga ttcgcacggc attgctcgcg gcggtcgggc acgatctgcg
     1501 caccccgctg gcgtcggtga aggcgagcgt cagcacgctg cgccagaccg acgtgacgtg
     1561 gtcgccgcag gaccagtcgg agctgctcgc caccatcgaa gagggcgccg accggctcaa
     1621 cggcctgatc agcaacctgc tcgacatgag caggctccag accggcgccg tgcaactgtt
     1681 cgtccggccg acgtcgctgg aggaggtcgc gccactcgtt gtgcaaggcg tggacgacgg
     1741 tgggttgctg caggtggaga tgtccgagga cctgccgctg ttcgcagccg atccggggtt
     1801 gctcgagcgc gcgctggcca acctcgttgc gaacgccctg cgttactcac cgccaggcca
     1861 gccgccgacg ctggcggccc gggccgtgca gaacggcaac gcgccgagct cagtgcagat
     1921 cgacgtcatt gaccacggcc ctggcgtgcc agccgagcag tgggaccgca tcgtgcagcc
     1981 cttcgagcag gtcggcgacc cgcgcagcgg caatggcgtc ggactcggcc tcgcggtcac
     2041 gaaagggttc gtcgaggcga tgggaggcaa gctcagcgcc gccgcgacac caggaggcgg
     2101 cctgacgatg agcatcgagt tggccgtcat cacggctggt tcagacggcg ggggtgagca
     2161 gccgtgacga gcgtgctcgt catcgacgac gaagccgcca tcctgcgcgc gctgcgcatc
     2221 aacttgtcgg cccgtgacta ccaggtcacg acggcgtccg acggtgcgtc cggtctcgct
     2281 gccatggcgc gcgagcggcc agacgtcgtg atcctcgacc tcgggctgcc cgacatggcc
     2341 ggcaccgacg tcattcgcgg tatccgtggt tggtcgagtg ttccgatcat cgtgctgtcg
     2401 gcgaacgaac aagaactgca gaaggtgcag gctctcgacg ccggtgccga cgattacgtg
     2461 acgaagccct tcggcatgaa cgaactgctc gcccgattgc gagcagctac ccgccgcgct
     2521 gcaccaggcg tcgacgagcc ggtcgtggtc acacccgagt tcaccatcga cctaggcgcg
     2581 aaacgggtgg tgcgcaagga cggccgcgac gtccggctca cgccgactga gtggcagctg
     2641 ctcgaggttc ttgcgcgcca cgaaggccgg ctcgtcagtg ggcgccagct gcttcaagac
     2701 gtgtggggcc cgacgtacgg cacagagacc aactacttgc gcgtctacat ggcacaactg
     2761 cgccgcaagc tcgagcccga tccggcgcgg ccgcgctacc tgatcaccga gccgggcatg
     2821 ggctaccgcc tgatgaccga ctgagcgtcg ctggcgtcga ttgcgagagg atcgccacat
     2881 gcgcactctt gtcacgcccg gcaaggacac cgatctcatc aagcttgagc tagccgacct
     2941 cgaacccggc gaatcgctga ccttcgtcgc tgatggcgag gcgctcatcg tgatcctgac
     3001 cggcgccctc gacgtcgaag tcgacggtgt accgatgggg agggccggcg gtcgtgc
//
LOCUS       DASWVF010000343         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_442574, whole genome shotgun sequence.
ACCESSION   DASWVF010000343 DASWVF010000000
VERSION     DASWVF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442574"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGD55_06155"
     CDS             <1..639
                     /locus_tag="VGD55_06155"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEY0869959.1"
                     /translation="DAGAGYDPTATGGSNLGPESIIDTLPDPSNSKDTGKQSLLTQVC
                     SRSIAVGKLEGVDGQRPYCTSDGVGAVLSVIHDTGLTGPIKSVVSVNQECPATPFVST
                     YDGAAVTCATFGVDYRIGKIVVVHGPGKGTGNAVPADAVTASASGLDPDISVAYANLQ
                     APRVAKARGISVDTVHKLISQHTVGRALGFMGNPGVDVLQLNLALDKLSGGA"
     gene            678..3260
                     /locus_tag="VGD55_06160"
     CDS             678..3260
                     /locus_tag="VGD55_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0869960.1"
                     /translation="MSGARGTLRIYLGAAPGVGKTFAMLGEGHRRAERGTDVVVGLVE
                     THGRPHTAEAIGDLEVIPRRTINYRGSDFTEMDVDAILTRHPKVALVDELAHTNVPGS
                     RNEKRWQDVEELLAAGITVLSTVNIQHLESLNDVVEKITGVPQRETIPDAIVRSADQV
                     DVVDLSPEALRRRMAHGNIYGPDKVDAALGHYFRIGNLTALRELALLWVADKVDDALQ
                     HYRHEHQIAETWETRERVLVAITGGPEGETLIRRAARIAARSGADLLALHVASSDGLT
                     GANPAMLAAQRLLVESLGGTYHQVVGDDISATLIAFAHAENVTQLVLGASRRSRLGAL
                     LNGAGIGSTTTRMSGDIDVHTVMHDQAGRRHTGPSMPRGLTVRRRITGAVLGAVLLPL
                     LTIVLANTRSSFNLSSDLLGYLLGVVAVALVGGLYSALATAVVSSLLINYYFTPPLYR
                     WTIADHNNIFAIVAFVIVAATVSLVVELAARRTSQAARASAESETLATLAGSVLRGQT
                     GVAALLEQVRETFQLTSVALLEHGTEGAEQEWTVVASVGAQPCQTPADADTEVPVGDR
                     FALAARGRPMAASDLRILGAFAAQAVTAVEQQRLAAAAEAAGPLEEADRMRTALLNAV
                     SHDLRTPLASAKAAVTGLRSADVAWTDTEQAELLATADESLDRLHRLVDNLLDMSRIQ
                     AGALSIRRQPVALHETVPIALDSLGPAARDIELDIPDSLPEVETDPALLERVIANLTA
                     NALRYSPRGTPPRVSASTHADSVELRISDRGPGIPESQRERMFTPFQRLGDTDNTTGV
                     GLGLALSLGLMQALGHTLTPEETPGGGLTMVLGMRISPAATAEPVQALAADSNAVPVP
                     EGRR"
     gene            3257..3952
                     /locus_tag="VGD55_06165"
     CDS             3257..3952
                     /locus_tag="VGD55_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0869961.1"
                     /translation="MTRVLVVDDEPQILRALSITLKARSYDVVVATSGKQALAIAAQS
                     SPDIVVLDLGLPDLDGIDVIRGLRGWSKVPIIVLSGRSQGVSKVDALDAGADDYVTKP
                     FNVDELLARLRAVTRRAPAGSDEVRAVIGDIKVELPGRRLSSRASGSADGVPDVRLTP
                     TEWQLLEVLIRNPGRLVTQREMLRSIDRADVGDQSHYLRQFMNQLRRKLEPDPARPRY
                     LMTEPGMGYRFQP"
     gene            complement(3912..4613)
                     /locus_tag="VGD55_06170"
     CDS             complement(3912..4613)
                     /locus_tag="VGD55_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0869962.1"
                     /translation="MYLLRVSVPDRPGALGALASAIGEAGGDITAVDVVERGPTAAVD
                     DVLVETADAESASTILAKLQALPAIVIEAWQPFTEGDQLRDGLDVVDGLGSTASRALA
                     AITRIAPAVLRARWVVLIDDVSGGVAITQASAGAPWVRWAALPWLPLATAAEVEADPS
                     WLPHEWGADPQLAAAPVGDSHMVLLAVRPIGPRFRHSETAKLANLAAIAALAAHSTQP
                     FGLKAGSDNPSLARS"
     gene            4810..6486
                     /locus_tag="VGD55_06175"
     CDS             4810..6486
                     /locus_tag="VGD55_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY0869963.1"
                     /translation="MEFLHNHIGYSTADAKVALLQTDAEFGGGRFSVYGAGASGPVFT
                     GELQPRGGVMRWRDWRYWEADFGAFETPGRYFVAIDGATPPVVTETFVIADNVLDGQV
                     VSDLVNYLKAVRCTGVFDIADRSCPVFGSTERVDVHGGWYDASGDTSKYLSHLSYANF
                     LNPQQTPQVVWNLIEGRLRLNETDLWMDDRLVDEALHGADFLVRMQSPEGYFYLTVFD
                     KWTKDVNERMVCSFTTQDGHRFDSYQAGYRQGGGSTIAALARASTLPRDGDFVRAAYL
                     AAAENGFAHLEKHNAEYLDDGTENIIDDYCALLAAVELFAATGDEIYRDAADLRLGRL
                     VDRQHPDGWFWADEAKTRSYVHAAEAGMPYAVLLRYIEVFAASDPRLLAAARQAVRRG
                     LQHELDVSAGDNPFSYPRQYVLVPGKPGRVQYFMAHENDSGYWWQGENARLGSLACAA
                     RWGAHYFADDEQFSAALRRYAQATLDWIFGLNPFDTCMMQGHGHNNPRYDLDKWNAPG
                     GVCNGITAGEFDEDDIDFKRAWETDPSQSWRWTEQWIPHGAWLFVALAHR"
     gene            6640..7122
                     /locus_tag="VGD55_06180"
     CDS             6640..7122
                     /locus_tag="VGD55_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0869964.1"
                     /translation="MPLDVNSTTMTDAGVPDAITMLRGSGLRATAARIAVLSEIASQP
                     HAPADEIASAARQRLGAVSMAAVYGVLNTLAAAGLIRRIEPAGSPARYETRIADNHHH
                     IVCRLCNAVADVDCAVGAAPCLAPSDTHGFVIDEAEVTWWGICGSCNDGATSNDSTSS
                     "
     gene            7148..>7597
                     /locus_tag="VGD55_06185"
     CDS             7148..>7597
                     /locus_tag="VGD55_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY0869965.1"
                     /translation="MSQDHPDAVVREMNEPEAEERECPVAHVRRAYPSEGGNNRDWWP
                     NQLNLKILRKHPDVANPMDADFDYAAAFKTVALGELRADIEQVMTTSQDRWPADFGHY
                     GPFFIRMAWHSAGTYRISDGRGGAGAGMQRFAPLNSWPDNVSLDKSRR"
BASE COUNT         1325 a         2511 c         2477 g         1284 t
ORIGIN      
        1 gacgccggtg caggttatga cccgaccgcc accggcggca gcaaccttgg gcccgagagc
       61 atcatcgaca ccctgcccga cccgtcgaac agtaaggaca ccggcaagca gagcctcctc
      121 acgcaggtgt gttcacgcag catcgctgtc ggcaagctcg agggcgtcga cgggcaacgg
      181 ccttactgca cgagcgacgg tgtgggtgcc gtgctttcgg tgattcatga caccggcttg
      241 actgggccta tcaagagtgt cgtatccgtc aaccaggaat gccctgcgac gccgttcgtc
      301 tccacctacg acggtgcagc ggttacctgc gcgacgttcg gggtcgacta ccgcatcggc
      361 aagatcgtcg tcgtacacgg gccaggcaag ggcaccggca acgcggttcc tgcggatgcc
      421 gtaaccgcca gcgcgagcgg tctcgatccc gacatctcgg tggcctatgc gaacttgcag
      481 gcaccgaggg tcgccaaggc acgcggcatc agcgtcgaca ccgttcataa gctgatctcg
      541 cagcacacgg ttggcagggc gcttggcttc atgggcaacc cgggcgtcga tgtcctgcaa
      601 ctgaatctcg ccctcgacaa gttgtccgga ggcgcatagc gcgcgccgga aatagtgtga
      661 ggttggaggt gtcgtcgatg tcgggtgcgc gtggcacgtt gcgcatctac ctcggcgcgg
      721 ccccaggcgt cggcaagacg ttcgcgatgc tcggtgaagg tcatcgccga gccgaacgcg
      781 gtaccgacgt cgttgtcggt ctggtcgaga cgcacggccg cccgcacacc gccgaagcca
      841 tcggcgatct cgaagtcatc ccgcgccgca cgatcaacta ccgcggcagc gacttcaccg
      901 agatggatgt cgacgcgatc ctgacccggc acccgaaggt cgcactggtc gacgagcttg
      961 cgcacaccaa tgttcccggc tcccgcaatg aaaagcgatg gcaggacgtc gaggagttgc
     1021 tcgccgccgg gatcaccgtg ttgtcgacag tgaacatcca gcatctggag tcgctgaacg
     1081 acgtcgtgga aaagatcacc ggtgtgcccc aacgcgagac catccccgat gcgatcgtga
     1141 ggtcggccga ccaggtcgac gtggtcgacc tctcacccga agcgctacgc cggcggatgg
     1201 ctcacggcaa catctacggc cccgacaagg tcgacgccgc gctcggccac tacttccgca
     1261 taggcaacct gactgcgcta cgcgagctcg ccttgttgtg ggtggccgac aaggtcgacg
     1321 acgcgctcca gcactaccgg cacgagcatc agatcgccga gacgtgggag acgcgagagc
     1381 gtgtgcttgt cgccatcacc ggtgggcccg agggcgagac gctgatccgg cgggcagcaa
     1441 gaattgcggc gcgcagtggc gccgatctgc tcgcgttgca cgttgcgtcg tcagacggcc
     1501 tgaccggcgc gaacccggcg atgctcgctg cgcaacgact gctggtcgaa tcactcggcg
     1561 gcacgtatca ccaggtcgtc ggcgacgata tttccgcgac gctgatcgcg ttcgcgcacg
     1621 ccgagaatgt gacccaactc gtgctcggcg catcgcgtcg gtcacgactg ggtgcgttgc
     1681 tcaacggtgc ggggatcggg tcgaccacga cgcggatgtc aggtgacatc gacgtccaca
     1741 ccgttatgca cgatcaggcc ggacgccggc acaccggtcc gtcgatgccc cgaggtctga
     1801 ccgtgcgccg acgcatcacc ggtgcggtgc tcggcgctgt gttgctgccc ctgctcacga
     1861 tcgtgctcgc caacactcgt agcagtttca acctttcgag tgacctgctc ggatatttgc
     1921 ttggcgttgt cgctgtggcg cttgtcggcg ggctgtactc cgcgctggca accgccgtcg
     1981 tgtcgagcct gctcatcaac tattacttca ccccgcctct ctacaggtgg accatcgccg
     2041 accacaacaa catctttgcg atcgtcgcgt tcgtgatcgt cgccgcaaca gtgagccttg
     2101 ttgtcgaact ggccgcgcgc cgtacctcgc aagccgcgcg cgcctcggcc gagtccgaaa
     2161 cgctggccac cttggccggc agcgtcttgc gtgggcaaac aggagttgcc gcacttctcg
     2221 agcaagtgcg ggagacgttc caactgacct ccgtggcttt gcttgagcac ggaaccgaag
     2281 gcgccgagca ggagtggacg gtcgtcgcgt cggtcggagc acagccgtgc caaaccccgg
     2341 ccgacgctga caccgaagtg ccggttggag atcgcttcgc gttggctgct cgcggacgcc
     2401 cgatggcggc gtccgacctg cgcattctcg gcgcattcgc agcccaagca gtgacggccg
     2461 tcgaacagca acggctggcg gcggcagccg aggcggccgg gccactcgaa gaggccgacc
     2521 gtatgcgcac ggcgttgctg aacgccgtca gccacgatct gcgcacgccg cttgcgtcgg
     2581 cgaaagcagc agtcaccggc ctgcgcagcg ccgacgttgc gtggaccgac accgagcaag
     2641 cagagctgct cgctaccgcc gatgagtcgc tcgatcggct gcaccggctc gtcgacaacc
     2701 ttcttgacat gagtcgcatc caggccggcg cgctctcgat tcgccggcag ccggtggcgt
     2761 tgcacgaaac ggtgccgatt gcgctcgaca gcctcgggcc tgcagcacgc gatatcgagc
     2821 tcgatatccc cgactcgctt cccgaggtcg agaccgaccc tgccttgctt gagcgggtga
     2881 tcgcgaatct caccgcgaac gcactgcgct actcacctcg cggaacaccg ccccgcgtat
     2941 cggccagcac acacgccgat tcggtcgagt tgcgaatctc cgaccgtggg ccggggattc
     3001 cggagagcca gcgcgagcgg atgttcacgc ccttccagcg actcggcgac accgacaaca
     3061 cgaccggcgt cggtctcggt cttgcgttgt cgcttggact catgcaggcg ttgggtcaca
     3121 cgctgacgcc cgaagagacg ccaggcggcg ggctgacgat ggtgctcggt atgcgaataa
     3181 gcccggctgc aaccgccgag cctgtccagg ctctcgcggc agactccaac gcagtgcccg
     3241 tacctgaggg ccgcagatga ccagagtgct ggttgtcgac gacgagccgc agatcttgcg
     3301 cgctctgagc atcacgttga aggcccgctc gtacgacgtc gtcgtcgcga ccagtggcaa
     3361 gcaggcgctg gcgattgctg cgcagtcgtc gcccgacatc gtggtgctcg acctcggcct
     3421 acccgatctc gacggcatcg atgtgatccg cggcctgcgc ggctggagca aggtgccgat
     3481 catcgtcttg tccgggcgca gccagggggt ctcgaaggtc gacgcgcttg atgccggcgc
     3541 tgacgactac gtgaccaagc cgttcaacgt cgatgagttg ctcgcccggc tgcgcgccgt
     3601 gacgcgccgt gcgcctgcgg gcagtgacga ggtgcgagcc gtcatcggcg acatcaaggt
     3661 cgagttgccc ggacgccgat tgagttcgcg ggcttccggc tccgccgacg gcgttcccga
     3721 cgtccggctg actcccaccg aatggcaact gcttgaggtg ctgatccgaa acccggggcg
     3781 ccttgtcacg caacgggaga tgcttcgatc gatcgaccgc gctgatgttg gcgaccaaag
     3841 ccactaccta cgacagttca tgaaccagtt gagacgcaag ctcgagcccg acccagcgcg
     3901 gccgcgctat ctcatgaccg agccagggat gggttatcgc ttccagcctt gagcccaaac
     3961 ggttgcgtcg aatgtgccgc cagagcggcg attgctgcga gattcgcgag cttcgccgtt
     4021 tcgctgtggc gaaaccgcgg tccgatcggt cggacggcga gcagcaccat gtgtgagtcg
     4081 ccgaccggcg ctgcggcgag ttggggatcg gcgccccact cgtgcggcag ccaggacgga
     4141 tcggcctcga cctcagctgc ggtcgcgagc ggcagccacg gcaacgctgc ccaccgcacc
     4201 cacggcgcac cggcgctcgc ttgcgtgatc gcgacccctc cgctgacatc gtcgatgagc
     4261 accacccacc gggctcggag caccgctggg gcgatgcggg tgatcgctgc gagcgctcgc
     4321 gatgccgtcg acccgagacc gtcgacgacg tcgagcccat cccttagctg gtcaccttcg
     4381 gtgaacggct gccaggcctc gatcacgatc gccggcagcg cctggagttt cgcgaggatc
     4441 gtgctcgccg actcggcgtc ggcagtctcg accaggacgt cgtcaacggc tgcggtcgga
     4501 ccccgttcga cgacgtcgac cgccgtgatg tcaccgccgg cctcgccgat cgcactggcc
     4561 aacgcgccga gagctccagg ccgatcagga acgctgacgc gcagtaggta caacacgaat
     4621 tccgctcccg tctctgaccg cttcagtcta tggttgcggc gccgtgtgtc ggcactgttc
     4681 aaccgccgtg acgttcggat ttcagcagcg tgccgacgtc gtttggcggg tgtcgctgtg
     4741 gtccctcgga gacgcactcg gggcgtgcgt gcatgatggc cggtgacacg aggagttcgg
     4801 aggaacagcg tggagttttt gcacaaccac atcggttatt cgaccgccga cgccaaggtc
     4861 gccttgctgc aaactgacgc cgagttcggt ggcgggcgtt tcagtgttta cggcgccggg
     4921 gcatcagggc ccgtcttcac gggtgagttg cagcctcgcg gcggcgtcat gcgctggcgc
     4981 gactggcgct actgggaggc cgacttcggg gcgttcgaaa cccccgggcg ctatttcgtg
     5041 gcgatcgacg gtgcgacgcc cccggtggtg acggagacat tcgtgatcgc cgacaacgtg
     5101 ctcgacggtc aggtcgtgtc cgacctcgtg aactacctga aagcggtgcg ctgcacgggt
     5161 gtcttcgaca tcgccgaccg gagttgtccg gtattcggca gcaccgagcg ggtcgacgtc
     5221 cacggtggtt ggtacgacgc atcgggtgac acctcgaaat acctctcgca cctgtcgtac
     5281 gcgaacttcc tgaatccaca gcagacccca caggtcgtct ggaacctcat cgagggccgg
     5341 ctcaggctga acgagaccga tctgtggatg gacgatcggc tcgtcgacga ggcgttgcac
     5401 ggtgccgact tcttggtgcg gatgcaaagt cccgagggct acttctacct caccgtcttc
     5461 gacaagtgga ccaaggacgt aaatgagcgg atggtctgta gcttcacgac gcaggacggg
     5521 caccgtttcg acagttacca ggccggctat cggcagggcg gtggcagcac gatcgctgca
     5581 ctcgctcggg cgagcacgtt gccacgcgac ggcgacttcg tccgcgcggc gtacctcgcg
     5641 gcggcagaaa acgggttcgc ccacctcgag aagcacaatg ctgaatacct cgacgacggc
     5701 actgagaaca tcatcgacga ctactgcgcg ttgctcgcgg cggtcgagct gttcgcagcc
     5761 accggcgacg agatttaccg cgacgcagcc gaccttcggc tgggtcgtct tgtcgatcgc
     5821 caacaccccg acgggtggtt ttgggcagac gaagcgaaga cccgcagtta cgtgcacgcg
     5881 gcagaggccg gcatgccgta tgcggtgctg ttgcgctaca tcgaggtgtt cgccgcgtca
     5941 gatccgagac tgctcgcggc cgctcggcag gcggttcggc gaggtctgca acacgagctt
     6001 gacgtcagtg ctggcgacaa cccgttcagc tatccgcgcc agtacgtgct cgtgcccggc
     6061 aagcctggcc gggtgcagta cttcatggcg cacgagaacg acagcggcta ctggtggcaa
     6121 ggcgagaacg cgcggttagg gtcgctcgcg tgcgcggctc gctggggtgc gcactacttc
     6181 gctgacgacg agcagttcag cgcggccctg cgccggtacg cacaggccac gctcgactgg
     6241 atcttcgggt tgaacccctt cgacacgtgc atgatgcagg gccacgggca caataacccg
     6301 cgctacgacc tcgacaagtg gaacgcgccc ggtggcgtct gcaacggcat cactgccggc
     6361 gagttcgacg aggacgacat cgacttcaag cgggcctggg agaccgaccc gtcgcagtct
     6421 tggcgctgga ccgagcagtg gatcccgcac ggcgcctggc tcttcgtcgc cctcgcccac
     6481 cgctaaccga tcggagtgga tcacgtttac gtgatcaacg cgcccgagga caggcgtagg
     6541 agggtgtcct cggggtccgg cgcctgtcct cggccagccg ataggacgga gtgagtccag
     6601 aacttgactc actccagaaa gccggattag ctgggcctcg tgccactcga tgtcaacagc
     6661 accaccatga cggacgccgg ggtgcccgac gccatcacga tgcttcgggg gagtgggttg
     6721 cgggcgacag cggctcgaat cgccgtgctg tccgagatcg cgagccagcc acacgcaccg
     6781 gccgacgaga tcgccagcgc cgcgcgccag cgtcttggtg cggtctcgat ggccgccgtc
     6841 tatggggtct tgaacacgct cgcggccgcg gggctgatcc gtcgcatcga gcccgcaggc
     6901 tcacctgccc gctacgagac gcggatcgct gacaaccacc atcacatcgt ctgccgactc
     6961 tgcaacgccg tcgcagacgt cgattgcgca gtcggcgccg cgccctgcct cgcgccgagc
     7021 gacactcacg ggttcgtcat cgacgaagcc gaggtgacct ggtggggaat ctgcggcagc
     7081 tgtaatgacg gcgccaccag caatgacagc actagctcct gacgaactga tcgcgaaagg
     7141 atctccgatg tcgcaggacc accccgatgc cgtcgtccgc gagatgaacg agccggaggc
     7201 cgaagaacgc gagtgcccgg tggcacacgt ccggcgtgcg tatccctccg agggagggaa
     7261 taaccgtgac tggtggccaa accagttgaa tctgaagatc ctccgcaagc accccgacgt
     7321 ggccaatccg atggacgccg acttcgacta cgccgccgcg ttcaagacgg tcgctctggg
     7381 cgaacttagg gccgacatcg agcaggtcat gacgacgtca caggaccggt ggccggccga
     7441 cttcggccac tacgggccgt tctttatccg gatggcgtgg cacagcgctg gcacctaccg
     7501 catcagcgac ggccgcggcg gtgccggcgc cgggatgcaa cgcttcgctc cgctgaacag
     7561 ctggcccgac aacgtgagcc tggacaagtc ccgccgg
//
LOCUS       DASWVF010000344         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_340383, whole genome shotgun sequence.
ACCESSION   DASWVF010000344 DASWVF010000000
VERSION     DASWVF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340383"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1107
                     /locus_tag="VGD55_06190"
     CDS             184..1107
                     /locus_tag="VGD55_06190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY0869966.1"
                     /translation="MCGETLIDLVHVDGEIWRALPGGSPANTAVALARLSTSTAMLAR
                     ISGDAFGVRLRERLVGDGVDVRYVVKAAEQSSMAVVDLGDDGSARYSFYLRDTADWQW
                     VTNDLPVAFDPSVTALHAGSMALLMPPGGVVLEAMLERERAQRVVSIDPNVRATICPD
                     PRVYRESVERWLGLAHIVKASVDDVKWLYPDRTPADVLAEWSTRGPSLVVFTRSADGA
                     LARLPSGLEVGVGGIDVEVVDTIGAGDTFTAGLLHAFDEAGLLTITALAQLSAEQVEE
                     ALRFAVLVSAVACTRAGSDPPYLTDLPALSS"
     gene            complement(1111..>2789)
                     /locus_tag="VGD55_06195"
     CDS             complement(1111..>2789)
                     /locus_tag="VGD55_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0869967.1"
                     /translation="ELRMVGATTLDEYRERIEKDPALERRFQPVLVGEPSVEDTIGIL
                     RGLKSRYEAHHKVQISDAALVAAATLSDRYITGRFLPDKAIDLVDEAASRLRMEIDSS
                     PVEIDELRRAVDRMKMEELALAHEDDAASRERLSRLRAELADRSEQLSALTARWDLEK
                     AGLNRVGELKQRLDDARGQAERAQRDGDFETASRLMYADIPALERSLAEAGASAEASD
                     AAGGRGAMVKEEVGPDDIAEVVGAWTGIPAGRLLEGETGKLLRMESELGRRLIGQKRA
                     VAAVSDAVRRARAGISDPDRPTGSFLFLGPTGVGKTELAKALADFLFDDERAIIRIDM
                     SEYSEKHSVARLVGAPPGYVGYEEGGQLTEAVRRRPYCVVLLDEVEKAHPEVFDVLLQ
                     VLDDGRLTDGQGRTVDFRNTILILTSNLGSMYLIDPTLDDDTRRDAVMTLVRGTFKPE
                     FLNRLDEVILFDPLDTDDLTRIVDLQIARLQARLADRRLTLSVTPAALEWLALTGFDP
                     LYGARPLRRLVQSAIGDQLARGLLSGDIRDGQTVQVDVAASKDALVIHAV"
BASE COUNT          491 a          882 c          893 g          523 t
ORIGIN      
        1 cgcgatctgc caaagcgcgc gggagtggtc gcaattccac attcggtgtt ttacgacaac
       61 attgagacag gacgcccgtt ggtgcggttt gctttctgca agcgcgacga agtgctgatc
      121 gaagcaatct ctcggttggg ccaactcgtt tcctagcctc gctggaggtc gtcggtgatt
      181 ttggtgtgcg gggaaaccct gatcgatctc gtccatgttg acggcgagat atggcgagca
      241 ttgcctgggg gatcgcctgc caacaccgcg gttgcgctgg cccgactaag cacgtcgacg
      301 gccatgctcg cgcgaatctc cggagacgca ttcggtgtgc ggttgcgcga gcgactcgtc
      361 ggcgatggtg ttgacgttcg ttatgtcgtc aaggctgctg agcaaagttc gatggcggtc
      421 gttgacctcg gtgatgacgg cagtgcccga tacagcttct accttcgcga cacagccgac
      481 tggcaatggg tcaccaacga tttgcccgtc gcgttcgatc cgtccgtgac ggctttgcac
      541 gcaggttcga tggcactgct gatgccgcct ggtggcgtcg ttctcgaagc gatgcttgaa
      601 cgcgagcggg cgcagcgcgt ggtgtcgatc gacccgaatg tgcgcgcgac gatctgtccc
      661 gatcctcggg tatacagaga atcggtcgag cgatggcttg gcctcgcgca tatcgtcaaa
      721 gccagtgtcg acgacgtgaa gtggctgtat ccagatcgca ccccagctga cgtgctagcc
      781 gagtggtcga cccgcggccc atcgctggtc gtgttcacca gaagcgcgga tggcgcgttg
      841 gcgcgactgc cgtcaggact tgaagtcggt gtcggcggca tcgacgtcga ggtggtcgac
      901 acgatcgggg ccggcgacac ctttacggcg ggccttcttc acgcattcga cgaagccggg
      961 ctgctcacca tcacggcgct cgcacaactg agtgcggaac aggtcgaaga ggcgttacgg
     1021 ttcgccgtcc tggtgtcggc agttgcgtgc acccgcgccg gatccgatcc gccatacctg
     1081 accgacctgc ccgccctcag cagctaggtc ctatacggcg tggatgacga gtgcgtcctt
     1141 cgaggcggcc acgtcgacct gcaccgtttg gccgtcacgg atatcacctg acagcaaccc
     1201 gcgcgcgagt tggtcgccga tcgccgactg cactaaccgc cgcaacggcc gggcgccgta
     1261 gagcgggtcg aagccagtca gtgcgagcca ctccaacgcg gcgggcgtca ccgacagcgt
     1321 gagacgtcga tcggccaacc gggcttgcag gcgggcgatc tgcagatcca cgattcgggt
     1381 gaggtcatca gtgtcgagtg ggtcgaacag gatcacttcg tcgaggcggt tcaagaactc
     1441 gggcttgaac gtgccacgca ccagcgtcat caccgcgtcg cgtcgggtgt cgtcgtcgag
     1501 tgtcggatcg atgaggtaca tcgagcctag gttcgaggtc aggatcaaga tggtgttgcg
     1561 gaagtcgacc gtgcgaccct gaccatcggt cagccgaccg tcgtcgagca cctgcaacaa
     1621 cacgtcgaag acctcagggt gagctttctc gacttcgtcg agcaagacga cgcagtacgg
     1681 acgacgacga acagcctcgg tgagttggcc gccctcttcg tagccgacat agcccggagg
     1741 cgcaccgacg agtcgggcga ccgagtgctt ctcggagtac tcgctcatgt cgatgcgaat
     1801 gatcgcgcgc tcgtcgtcga acagaaaatc ggcgagtgcc ttcgccaatt cggtcttgcc
     1861 gactccggtc ggaccgagga acaggaacga cccggtcggc cgatcggggt cggagatgcc
     1921 agcccgagcc cgacgcaccg catcggaaac ggctgccacc gctcgctttt ggccgatcaa
     1981 gcgacggccg agttcggatt ccatgcgcag caacttgccg gtctcgcctt cgagcaggcg
     2041 gccggccgga atgcctgtcc aggcaccgac gacctctgca atgtcatcgg ggccaacttc
     2101 ttccttgacc atcgcgccac ggccgcccgc agcgtcagat gcctcggccg acgcgccagc
     2161 ttcggcaagc gaccgctcaa gtgcaggaat gtcggcgtac atcaaccgag acgctgtctc
     2221 gaagtcaccg tcgcgctgtg cgcgttcagc ttgcccgcgc gcgtcatcga ggcgttgctt
     2281 cagttcgccg acgcggttga ggccggcctt ctcaaggtcc catcgcgccg tcagcgcgct
     2341 cagctgctca gagcggtcgg cgagctccgc ccgtaaccga gaaagacgtt cacgactcgc
     2401 ggcgtcgtcc tcgtgggcaa gcgcgagttc ttccatcttc atccggtcga cggcccgccg
     2461 aagttcgtcg atctcgaccg gcgacgagtc gatctccatg cgcaaccgtg acgcggcctc
     2521 gtcgacgaga tcgatcgcct tgtcgggcag gaagcgaccg gtgatgtagc ggtcggacaa
     2581 cgtggctgcg gccaccaatg cggcgtcgga gatctgcact ttgtggtgcg cctcgtagcg
     2641 cgacttcaac ccacgcagga tgccgatggt gtcttcgacc gagggctcgc cgaccaacac
     2701 cggctggaag cgtcgctcca gcgccggatc cttctcgatc cgctcccggt actcgtccaa
     2761 agtcgttgca ccgaccatgc gcaactcac
//
LOCUS       DASWVF010000345         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_213283, whole genome shotgun sequence.
ACCESSION   DASWVF010000345 DASWVF010000000
VERSION     DASWVF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213283"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..407)
                     /locus_tag="VGD55_06200"
     CDS             complement(84..407)
                     /locus_tag="VGD55_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869968.1"
                     /translation="MAKFMYLYRGPAPSMTDLTPERAAERMAAFGAWMAGVGTALIDV
                     GNPFGLSGSIRDDGTEGPAGDLIGYTIVEAVDLDGAKALTDSLPFLSNNDGKCVVEIF
                     ELLSM"
     gene            706..1056
                     /locus_tag="VGD55_06205"
     CDS             706..1056
                     /locus_tag="VGD55_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0869969.1"
                     /translation="MGHPVIHAEIRSADPDATRAFFGELFGWKVASEGAFPGYTFIDT
                     GAEGGTYVAISPRQSADDEVLFFVGVEDVDATLAKAEKLGGTIIQPAQHVPGTSFGVF
                     ADAQGHRVGVAANG"
     regulatory      1092..1168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1184..1903
                     /locus_tag="VGD55_06210"
     CDS             1184..1903
                     /locus_tag="VGD55_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY0869970.1"
                     /translation="MSDSDRMVGHVVGLWRYPVKSMGAEALVEADVSWNGLAGDRRWA
                     FIRDGAVRSGFPWLTLRELPELSHYRPSFVEPERPNASRTMVRTPAGDEFDVTDPVLA
                     AELGVGVRIIKHDRGVFDTMPLSLITTQTIAGLGALIGSQLDALRFRPNLLIEASTDD
                     AFPEDAWVGSVLHVGGLQMRVDARDQRCVVVNIDPATRSRNPAVLRAIANDRQACVGV
                     YGSTVQPGRIAVGDPVIATVS"
     gene            complement(1912..2982)
                     /locus_tag="VGD55_06215"
     CDS             complement(1912..2982)
                     /locus_tag="VGD55_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0869971.1"
                     /translation="MTKFSFVANASTDHDRGTGVADRVTTTGGEASAFEYPDRLQNPL
                     ERVRLKGAIFLRAEYREPWAYESMSGPATANFLQRGSDRVVLFHVVASGTCWVAVDGG
                     EKHWASAGDVIVLPYGDQHQMGGLEVADVVPITAFMPPAPWTQMPVLRHGQDGRQTDL
                     VCGYLHSEDTLFNPAMRALPSVFVVRPPAGAAAQWVHANIAYAMQQTSASPPGQGGIP
                     TRLSELLLIEVLRQHLASAPAVERGWAAALRDPVVAPALALLHRRPQHKWTVAELAQA
                     ASVSRSLLDARFRAVLGRSPIRYLTQWRMHVAQNLLATTDLSVLAVARRVGYDAEEAF
                     SRAFKRDIGSSPSVWRTAHYSH"
     gene            3022..3273
                     /locus_tag="VGD55_06220"
     CDS             3022..3273
                     /locus_tag="VGD55_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY0869972.1"
                     /translation="MPLFMDVHTIEGPVGLADVAHAHEADLKIQGDFGVNYKSYWVDE
                     ASGKIFCLVEAPNAEAAATVHRQAHGLVADEIYLVAEGA"
     gene            3502..3792
                     /locus_tag="VGD55_06225"
     CDS             3502..3792
                     /locus_tag="VGD55_06225"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEY0869973.1"
                     /translation="MRIGFSVFMMAVGAVLTFAVKATAKGVNIHTVGVILMIAGAIGL
                     IVTLTVFAPRRRESVVHERVVQGGIAPVAPAAVAAPAANVTQERTVENRDVY"
BASE COUNT          689 a         1245 c         1291 g          704 t
ORIGIN      
        1 aacgagtgtg gcttatgaac caccacagtt ggttccacaa agcggcgggt agtcctccgg
       61 ttcgacgcgt gcgccgcctg cctctacatt gacagcagtt cgaagatttc cacgacgcac
      121 ttcccgtcgt tgttcgacag aaacgggagg ctgtcggtga gcgccttcgc gccgtcaagg
      181 tcgacggcct cgacaatcgt gtatccgatc aagtcgccag ctggtccctc ggtaccgtca
      241 tcgcgaatcg acccgctcaa accgaacggg ttgccgacgt caatgagcgc tgtgccgacg
      301 cccgccatcc aggcgccaaa cgcagccatc cgctcggccg cccgctccgg cgtcaagtcg
      361 gtcatcgaag gcgctgggcc acggtaaagg tacatgaact tcgccatgtc gatcgtcctt
      421 tcgggttggt gcgcgtagca cgccgtcagc cgatattacg aacggcgccg cgccacacgg
      481 acacctgcgc gtaagagttc tcggttggcc ccatggcgcg ggccgcagca tcacctgcca
      541 tgggcagacc tcgaccgttg aagaaaccgt tgaccacact ggcgtcgtca tgcgtcgcga
      601 gctgccgagc tcgcaagaat cggagtagtc gcgcctgtcg attttgcgac ggctcgttcg
      661 tcgtggagat gtccaccgcc gttagatcga ttgaggagca tcgctatggg ccaccctgtg
      721 attcacgccg agatccgttc tgctgacccc gacgcgacgc gcgccttttt cggcgagctg
      781 ttcggttgga aggttgcgtc cgagggcgcg ttccccggct acacgttcat cgacacgggc
      841 gccgaaggcg gcacctatgt cgcgatcagc ccgcgccaaa gtgctgacga cgaggtgctc
      901 ttcttcgtcg gcgtcgaaga tgtcgatgcc accctcgcca aggccgaaaa gcttggcggc
      961 acgatcattc agcccgcgca gcacgtccct ggcacgagct tcggtgtctt cgccgacgcg
     1021 caaggacatc gggtcggcgt cgcagcgaac gggtagttga caacgcggct tcgatcgggc
     1081 gaggctgctt tcgggccgag gggcgctgca acggacctag tccgtcacgc tcggctcgca
     1141 cgcttgtcgg caagggcgcc tcctggtggg aggtgccctt cgcatgtccg attccgatcg
     1201 catggtgggc cacgttgttg gcctgtggcg ctacccagtc aagtcgatgg gcgccgaagc
     1261 tctcgtcgaa gcggacgtgt catggaacgg cctcgccggt gaccgccgct gggcctttat
     1321 tcgggacggc gcggtgcgca gcggctttcc ttggttgacg ctgcgagagc ttccggaact
     1381 gagccactac cgcccgtcgt tcgtcgagcc tgagcggcct aacgcgtcgc gaacgatggt
     1441 ccgcaccccc gcgggcgacg agttcgacgt caccgacccg gtgctcgccg ccgagctagg
     1501 tgtgggagtt cgcatcatca agcacgaccg tggggtgttc gacaccatgc cgctgtcgct
     1561 gatcacgacc cagaccatcg ctggcctcgg cgcgctgatc gggtcgcagc tcgatgcgtt
     1621 gcggttccga cctaacctgt tgatcgaggc gagcacagac gatgcctttc ccgaggacgc
     1681 gtgggtcggt tccgtgctgc acgtcggcgg cttgcagatg cgtgtcgacg cgcgagacca
     1741 gcgatgcgtc gtggtgaaca tcgaccctgc gacaaggagc cgcaacccag cggtgttgcg
     1801 tgcgatcgcc aacgaccggc aggcgtgcgt cggtgtctac ggctcgactg tccaaccggg
     1861 gcggatcgct gtcggtgacc cggtgatcgc gacggtcagt taggtcaggc gtcagtgcga
     1921 gtaatgggcc gttcgccaca cgctcggcga gctgccgata tcgcgtttga acgctcgact
     1981 gaacgcctcc tcggcgtcgt agccgactcg gcgggcgacg gcgagaacac tcaggtctgt
     2041 cgttgccagc aggttctgcg ctacgtgcat gcgccactgc gtcaggtatc gaatcggcga
     2101 acgcccgagc acggcacgaa atcgggcgtc gagcagcgag cgggaaaccg acgccgcctg
     2161 cgccaattcg gccacggtcc acttgtgctg cggtcggcga tgaagcagcg ccagcgcggg
     2221 tgctaccacg ggatcgcgca gcgcagcggc ccagccacgt tcgacggcgg gtgccgacgc
     2281 cagatgctgc cggagaacct cgatcagcag caactcgctc aagcgggtgg ggatgccacc
     2341 ctggcccgga ggtgacgccg acgtctgttg catcgcgtac gcgatgttgg cgtgaaccca
     2401 ctgcgccgct gctccagcgg gtggacgcac gacgaagacc gaaggcagtg cgcgcatcgc
     2461 ggggttgaac agcgtgtcct cactgtgcag gtacccgcac acgagatcgg tttgtcgacc
     2521 gtcctggccg tggcgcagga ccggcatctg tgtccagggc gcgggtggca tgaatgcggt
     2581 gatgggaacg acgtcggcaa cttcgagccc acccatttga tgctggtcgc cgtatggcag
     2641 cacgatgacg tcgccggcgc tggcccaatg cttctcacca ccgtcgacgg cgacccagca
     2701 cgtaccggat gccacgacgt ggaacaacac aacccggtcg ctgccacgct ggaggaaatt
     2761 cgcggtggcc ggcccggaca tcgactcgta cgcccacggc tccctgtact cggcgcgcag
     2821 gaagatcgcg cccttcagcc gtacccgctc aagggggttc tgcaagcggt cggggtactc
     2881 gaacgccgaa gcctcgccac cggtcgtggt tactcgatca gcaacgccgg tgccgcggtc
     2941 atggtcggtt gatgcgttcg ccacgaaact gaactttgtc atatcaacga gcaagccttt
     3001 ggctgagaga gaagtagcac aatgccactg tttatggacg ttcacacgat cgaagggccg
     3061 gtcggcctgg ctgacgtcgc ccatgcgcac gaagcggatc tgaagataca gggagacttc
     3121 ggcgtcaact acaagagtta ctgggtcgac gaggcgagcg gcaagatctt ctgccttgtc
     3181 gaggcaccca acgccgaggc ggccgcgacc gttcaccggc aggcgcacgg cctggtcgcc
     3241 gacgagatct acctcgtcgc tgagggcgcc taatcccgac ggagcggcgg cccggccacc
     3301 caccgccggg gctgctgctc taggcgcccg aagatgcgtg gaggtcgtgg gcactcctgc
     3361 gagcggcacc gtcaacacga acgaacgcac cgacagcggg acggcggctc gctgcgcttc
     3421 gtttcagcgc gtggcgatcg ggtaattgga gttcatccgg ccgcgctgcg gcctgagctc
     3481 tattcacagt tggaggtttc tgtgcgcatc ggattctcgg tgttcatgat ggctgtcggt
     3541 gcagtgctga cattcgctgt caaggcgacc gccaagggag tgaacatcca cacggtcggc
     3601 gtcatcttga tgattgccgg cgcgatcgga cttatcgtca cacttacggt ctttgcgccc
     3661 aggcgcagag aaagtgttgt gcacgaacgc gttgttcagg gcgggattgc tccagttgct
     3721 ccggcagctg tggccgctcc tgccgcgaat gtgacgcaag aacgcaccgt ggaaaaccgc
     3781 gacgtctact aagaacgcac ctgctcagaa cgccgagtgt gcgcaatcgg ggcgagttga
     3841 ccttcgggtg gccccgattg cgcacactcg atgacgggga acgccagtga gacggggccc
     3901 gtacgtagtg aggcggcgac cgttacgac
//
LOCUS       DASWVF010000346         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_213311, whole genome shotgun sequence.
ACCESSION   DASWVF010000346 DASWVF010000000
VERSION     DASWVF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213311"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /gene="thrS"
                     /locus_tag="VGD55_06230"
     CDS             <1..1231
                     /gene="thrS"
                     /locus_tag="VGD55_06230"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0869974.1"
                     /translation="MLAEAEKRDHRKLGLELDLFSFPEELGSGHAVFHPKGGVIRKVM
                     EDYSRAKHLEAGYEFVNTPHITKGKLYEISGHLDWYADGMYPPMELDGGTNYYLKPMN
                     CPMHILIYRARGRSYRELPLRMFEFGTVYRYEKSGVIHGLTRVRGMTQDDAHIFTTIE
                     NMRDELRKLLTFVLELLRDFGLGDFYLELSTKNPEKFVGDDDVWETATATLAEVAAES
                     GLELVPDPGGAAFYGPKISVQARDAIGRTWQMSTIQVDFNLPERFELEYQGADGERHR
                     PVMIHRALFGSIERFFAVLTEHYAGAFPPWLAPVQAIGIPITDAHVSYLDDVVGPALR
                     AAGVRFEVDASDERMQKKIRDAQKQKVPFMLIAGDDDIAAGAVSFRYRDGTQKNGVQL
                     PDAVAEICDAIERRIQV"
     gene            1228..1803
                     /locus_tag="VGD55_06235"
     CDS             1228..1803
                     /locus_tag="VGD55_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY0869975.1"
                     /translation="MTETGPQGDVDIIGAVGVPDGFERMWTPHRMAYLNGENKPQGDP
                     NDPLLCPFCVIPTKSDEDALIVARGTLVYVVLNLYPYNAGHVLVVPYRHVADITELTA
                     DESAEFFLFTQQSVATLRSATTAHGFNVGMNLGSVAGAGIAAHLHQHVVPRWGGDSNF
                     MPIIGRTRVLPQLLSDTRKVLADAWPGHSSL"
BASE COUNT          360 a          615 c          589 g          361 t
ORIGIN      
        1 gatgctggct gaagccgaga agcgcgacca tcgcaagctc gggctcgaac ttgacctgtt
       61 cagcttcccc gaagagctcg gctctggcca cgcggtcttc catcccaagg gtggcgtgat
      121 ccgcaaggtg atggaggact actcgcgggc caagcacctc gaagcgggtt acgagttcgt
      181 caacactccg cacatcacca agggcaagct gtacgagatc tcgggccacc tcgactggta
      241 cgccgacggc atgtatccgc cgatggaact cgacggtggc accaactact acctcaagcc
      301 gatgaactgc ccgatgcaca tcttgatcta tcgcgcgcgg ggccggtcgt atcgcgaact
      361 gccgctgcgg atgttcgagt tcggcaccgt ttaccggtac gagaagtctg gcgtgatcca
      421 cggcttgacg cgagtccgcg gcatgaccca ggacgacgcg cacatcttca ccacgatcga
      481 aaacatgcgc gacgaactgc gcaaactcct taccttcgtg ctcgagctgt tgcgcgactt
      541 cggactcggc gacttctacc ttgaactgtc gacgaagaac ccggagaagt tcgtcggcga
      601 cgacgacgtg tgggaaaccg caaccgcgac gctggccgaa gtcgcggctg aatcgggcct
      661 cgaacttgtg cccgacccgg gcggcgccgc cttctatgga ccgaagattt ccgttcaggc
      721 gcgcgatgcg atcggccgca cctggcagat gtcgaccatc caggtcgact tcaacctgcc
      781 cgagcggttc gaactcgaat accaaggggc ggacggcgag cggcaccggc cggtcatgat
      841 ccatcgcgcc ttgttcggct cgatcgaacg cttcttcgcc gtgctgaccg agcactacgc
      901 cggcgccttc ccaccgtggc tcgctccggt gcaggccatc gggattccga tcaccgatgc
      961 ccacgtgtcg tatctcgacg acgtcgtcgg cccagcgctg cgagcggccg gcgtgaggtt
     1021 cgaggtcgac gcgtctgatg agcgcatgca gaagaagatc cgtgacgcac aaaagcagaa
     1081 ggtgccgttc atgctgatcg ccggcgacga cgatattgct gccggtgcag tctcgttccg
     1141 ctatcgcgat ggcacccaga agaacggtgt gcaactcccc gacgccgtcg ccgagatttg
     1201 cgatgccatc gagcgcagga ttcaggtgtg accgaaaccg gcccacaggg agacgtcgat
     1261 atcatcggcg ccgtcggcgt ccccgatggt ttcgaacgga tgtggacacc gcaccggatg
     1321 gcttacctca acggtgaaaa caagccgcag ggtgatccga acgatccgtt gctctgcccg
     1381 ttctgcgtca ttcccacgaa gtcagacgaa gacgctttga tcgtcgcgcg cggcacgctg
     1441 gtgtacgtcg tcttgaatct ctatccctac aacgcagggc atgtgctcgt cgtgccgtat
     1501 cgccacgtgg ccgacatcac cgagctcacc gccgacgaga gcgctgagtt ctttctcttc
     1561 acgcagcagt cggtcgccac attgcgttcg gcgacgaccg cccacggatt caatgttggc
     1621 atgaacctcg gaagtgtcgc gggcgcgggg attgccgcgc atctgcatca gcacgtcgtg
     1681 ccgaggtggg gtggcgattc gaacttcatg cccatcatcg gccgcacccg ggtgttgccg
     1741 cagttgctga gcgacacccg caaggtgcta gccgacgcct ggccaggtca ctcctctttg
     1801 tgagccgcga tgaccttcgt tgctagctgt ggcggcatcg gctcgtagcg caggttgtgc
     1861 cgggtgaagt tgcccgtgcc gtgcgacagc gaccgcagat cgaccgcgta tctggtgatc
     1921 tccac
//
LOCUS       DASWVF010000347         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_187794, whole genome shotgun sequence.
ACCESSION   DASWVF010000347 DASWVF010000000
VERSION     DASWVF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187794"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3223)
                     /locus_tag="VGD55_06240"
     CDS             complement(<1..3223)
                     /locus_tag="VGD55_06240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0869976.1"
                     /translation="METSPTIGVLSPFVSGFYFGGIIRGVAGAVGEAGARLVAIQTSD
                     PGVVYSERPDASPHPHAAAWEHLAGCIVVIDAATPAYLEAIRHSGKPVVLISKLIPGV
                     ICPTVQPDNKEGVREAVRHLISHGHTAIAFVGMMHQDDIRERYEAYAETLVEAGIEPD
                     PTLVFATADNDENCGFLAGQAMIANGMRSTAVMVATDVNAFGLMQALTAAGLELPRDQ
                     AIVGFDDVDAATFIKPALTSIRQDFGALGRLAATLLLDKLAGIEVANGRHEASTLFVI
                     RESCGCTQDVGGALTAGVKSSAATRRRALEDQLDRTLTADTGAPGRPAIVATAAASIV
                     ELIESAWGGDVTAPTPKLQGELEALYQRSPRNQTVRGVMAAARDFATELMAARVTDAD
                     DTDGPRRVAAQIIRLMLVLSAIHSQDQFNESTYFQNALGTQYAVAMDLLRSHEEDPRS
                     LQWLERTPVRAGCLGLWSEAPGELAPGADPTLAIVGAFDREAGTDSHRGRPGLDETVA
                     ASAFPPVQLLELADEAVDDVVFVLPVKVRSSDWGLLSIVGQIDSKVASGRETLNQWAA
                     LLTVALDHQANVASLEEQREKVERSYVRERGLVEEIRLSEERYALAAEAASGGLWDWD
                     LVNDVVFYSARWIALLECPGDAIGNTSEDWFGRVHPDDLSSLLSAIEEHLVGRSSAVE
                     LEHRIRRGDGSYLWVLCRALVIRGVDDVPIRMVGSLTDVHERKELESQLRHAALYDPL
                     TGLPNRTLFLDRLRVTMTRASRHSDHRFAVLFLDLDGFKLVNDSLGHLLGDALLSSVA
                     ERISADLRPSDTASRFGGDEFAILLDDITAPHNPVAVAERLQDRLARPFHVGGHEIFV
                     TASIGIASSSTGYQSAEDVLRDADTAMYRAKATEKGTHAIFDSGMHTRAVSRLRVEAE
                     FRQAIDGGQLRLYYQPIVAIRTGRTLAFEALVRWEHPTRGLLGPAEFLPIAEETGLIV
                     PMGRWILKEACRQIAEWKRAGAPADLRVSVNVSNKQFWHGGLLEDVQQCLREADLEPR
                     NIALEITETVVMHNADLAERMLMDLHESGFALHIDDFGTGYSSL"
     gene            complement(3296..>3994)
                     /locus_tag="VGD55_06245"
     CDS             complement(3296..>3994)
                     /locus_tag="VGD55_06245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0869977.1"
                     /translation="VLFSAYHLKPVVARLIAVEVVIAETILTVLIDPRGAVIEDLVGV
                     SLILVGIMLTLVKARDRVDHAINSLRYEAEHDAVTGLLSRRAFDADIEQLDAEQPASL
                     ILVDIDDFKTVNDTFGHVVGDQTLRIVADALTANSRQGDRAYRLGGDELAVLMSGIVP
                     ASALRRAEVIRRAVETAPTAFVVRRERGAVTVSLGVASLPPHAPRKSELLRAADAAMY
                     QAKSSGRNRVVAAA"
BASE COUNT          701 a         1331 c         1281 g          681 t
ORIGIN      
        1 cgagcgacga gtagccagtg ccgaagtcgt cgatgtgcag cgcgaagccg ctctcgtgca
       61 gatccatcag catccgctcg gcgagatcag cgttgtgcat cacgactgtt tcggtgatct
      121 cgagagcgat attgcgcggc tcgagatcgg cctccctcaa gcattgctgc acatcttcga
      181 gcaggccgcc gtgccagaac tgcttgttcg acacgttgac gctaacgcgg agatctgcgg
      241 gcgccccggc tcgcttccac tccgcgatct gccggcacgc ttccttgaga atccaccgac
      301 ccatcggcac gatcagccca gtttcctcag caatgggaag gaattctgct gggccgagca
      361 ggccgcgagt cgggtgctcc caccgaacca aggcttcgaa agcaagggtg cgcccggtgc
      421 ggatcgccac gatcggctgg taatacagcc gcaactgccc gccgtcgatc gcctggcgaa
      481 actccgcttc gacccgcagc cggctgacgg cgcgcgtgtg catgccggag tcgaagatcg
      541 cgtgagtgcc cttctcggtc gccttcgccc ggtacatcgc ggtatcggca tcgcgcagca
      601 cgtcttcggc cgattgatag ccggtgctgc tcgaggcgat accgatgctc gccgtgacga
      661 agatctcgtg accaccgacg tggaacggtc gagcgagcct gtcctgtagt cgctccgcga
      721 cggccactgg attgtgcgga gccgtgatgt cgtcgagcag gatcgcgaac tcgtcgccac
      781 cgaaacgcga cgccgtgtca gacgggcgca ggtcggcaga gattctttcg gcgacactgg
      841 aaagaagcgc gtcgccgagc agatgcccga ggctgtcgtt gacgagcttg aacccgtcga
      901 ggtcgaggaa cagcaccgcg aatcggtgat cggagtgccg actcgctcgc gtcatcgtca
      961 cacgcaaccg gtcgaggaac agggtgcggt tcggcaaccc cgtcaagggg tcgtacaacg
     1021 cggcatggcg gagctggctt tcaagctcct tgcgttcgtg gacgtcggtc agtgacccga
     1081 ccatgcggat cggtacgtcg tccacgcccc tgataaccag cgccctgcac aagacccaca
     1141 ggtagcttcc gtcgccgcgc cggatgcgat gctcgagttc gacggccgac gagcggccga
     1201 ccaggtgctc ttcgatcgcc gagagcaagc tcgacagatc atctggatga acgcggccga
     1261 accagtcttc gctggtgttg ccgatcgcgt cgccagggca ttcgaggagt gcgatccacc
     1321 gcgccgagta gaacacgacg tcgttgacga gatcccagtc ccacaaccca ccgctcgcag
     1381 cctcagcggc gagggcatag cgctcctcgc tcaacctgat ctcttcgacg agcccgcgtt
     1441 ctcgtacgta cgagcgctcg accttctcgc gttgctcctc gagtgacgcg acgttggcct
     1501 ggtggtcgag cgcgaccgta aggagcgccg cccactggtt cagggtttct cgccccgaag
     1561 caaccttgct gtcgatctgg ccgacgatgc tgagcagccc ccagtcactc gatcgcactt
     1621 tcaccggcag cacgaacacg acgtcgtcga cggcctcatc ggcgagctcg agcaactgaa
     1681 caggcgggaa cgccgatgcc gcgaccgtct cgtcgaggcc cggccgcccg cggtgtgagt
     1741 cggtgccggc ctcacggtcg aacgcaccga cgatcgctag cgtcgggtcg gcgccaggag
     1801 caagctcgcc gggcgcctcc gaccacagcc caagacagcc ggcgcgaacc ggcgtgcgct
     1861 cgagccattg cagcgaccgc ggatcttctt cgtggcttcg gagcaggtcc atcgcgaccg
     1921 cgtactgcgt gcccagcgcg ttttggaaat acgtgctctc gttgaattgg tcttgggagt
     1981 ggatcgccga cagcaccagc atcaggcgaa tgatctgcgc cgcgacccgc cgcgggccgt
     2041 cggtgtcgtc agcgtcagtg acgcgcgccg ccatcaactc ggtggcgaag tcgcgcgcgg
     2101 cggccatgac gcccctcacg gtctggttgc gtggactgcg ctggtacagc gcttcgagtt
     2161 cgccctgaag cttcggcgtc ggcgccgtga cgtcaccgcc ccacgccgac tcgatcagct
     2221 cgacgatcga cgcggctgcg gtggcgacga tcgccggtcg gcccggggcg ccggtatcgg
     2281 cggtgagggt gcgatcgagc tgatcctcca gagcgcgacg acgtgtggcc gccgagcttt
     2341 tcacccctgc cgtcaaagca ccgccgacgt cctgggtgca gccgcatgac tcgcggatca
     2401 cgaacagcgt cgatgcctcg tgccggccgt tggcgacctc gataccggcg agtttgtcga
     2461 gaagcagcgt ggcggccaac ctgccgagcg cgccgaagtc ctggcggata ctcgtcaatg
     2521 ccggcttgat gaaggtcgct gcgtcgacat cgtcgaatcc gactatggcc tgatcgcgtg
     2581 gaagttcgag acccgctgcg gtcaacgcct gcatcaaccc aaacgcgttg acatcggtcg
     2641 ccaccatcac ggcggtcgat ctcatcccgt tggcgatcat ggcctgaccg gcaaggaatc
     2701 cgcagttctc gtcgttgtcg gccgtggcga acacgagagt cgggtcaggc tcaatgcctg
     2761 cttcgacgag tgtctcggca taagcctcgt agcgctcgcg aatgtcgtct tggtgcatca
     2821 tcccgacgaa cgcgatggca gtgtgcccgt gactgatgag gtgtctgacc gcttccctga
     2881 cgccctcctt gttgtctggc tgcacggtcg ggcagatgac accgggtatg agcttgctga
     2941 tcaggacgac cggcttgccg gaatggcgga tcgcctcgag gtaggcaggg gtagcggcat
     3001 cgatgaccac gatgcagccg gcgaggtgct cccacgccgc cgcgtggggg tgcggcgagg
     3061 catcgggtcg ctcggagtag acgacacccg ggtcggacgt ctggatcgct accagccgcg
     3121 caccggcctc gcccaccgcg ccagcaacgc cgcgaatgat gccgccgaaa tagaagccac
     3181 tcacgaacgg cgacagcaca ccgatcgttg gcgaggtctc catggaagcc ctatcggccc
     3241 aggcgaccgg cttctgagcg tgaattgcgg tgacctgcga tgcctcacga ccccgtcagg
     3301 cagccgcgac gactcgattg cgtcctgagc tcttcgcctg gtacatcgcg gcgtcagcag
     3361 cccggaggag ctctgatttc cgaggggcgt gaggcggcag cgacgccacc ccgaggctga
     3421 ccgtgaccgc tccccgctcg cgcctcacca caaaggcggt cggcgcggtc tcaaccgccc
     3481 gccggatcac ctccgcgcgc cgcaaggcgc tggcagggac gatcccgctc atgagcacgg
     3541 ccagctcgtc cccgccgagg cggtaggcgc ggtcgccttg gcggctgttc gccgtcagtg
     3601 catcggcaac gatccgaagt gtctggtcac cgaccacgtg cccgaacgtg tcgttgaccg
     3661 tcttgaagtc atcgatgtcg acgaggatca aggacgccgg ctgctcggcg tccaactgct
     3721 cgatatcggc gtcgaaggcg cgcctgctca gcaggcctgt gacggcatcg tgctcggcct
     3781 catagcggag cgagttgatc gcgtggtcaa cgcgatcacg cgctttcacc agcgtgagca
     3841 tgatcccgac gagaatcagg gacacgccga ccaagtcctc aatcaccgca ccgcgcgggt
     3901 cgatcagcac ggtcaagatc gtctccgcaa tcacgacctc gacggctatc aatcgagcaa
     3961 cgaccggctt caggtggtac gccgagaaaa gcac
//
LOCUS       DASWVF010000348         5476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_238704, whole genome shotgun sequence.
ACCESSION   DASWVF010000348 DASWVF010000000
VERSION     DASWVF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238704"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGD55_06250"
     CDS             <1..384
                     /locus_tag="VGD55_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEY0869978.1"
                     /translation="IDVRRIETLKDGQEAVGNRTRAKVVKNKCAAPFRQAEFDIIYNQ
                     GISREGSLIDMGVDNGIVRKSGAWYTYEGDQLGQGKENSRTFLKDNPDLADEIAKKIK
                     EKLGIGPRLDVDVPIDLTVPVPIDL"
     gene            504..1082
                     /locus_tag="VGD55_06255"
     CDS             504..1082
                     /locus_tag="VGD55_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY0869979.1"
                     /translation="MARSIGLDLLDASPKTRAQLAEAMRRRGVPDAAATEVLDRFDEV
                     GLIDDAAFADAWVTSRQAGRGLAPRALANELRQRGVPQELIADALGRVDSDDVESAAR
                     DLVTRRARSSAGLPTQVRMRRLVAMLNRKGYSGELAVRVVREVLAVDDRDPDSDPDRD
                     PEGGPGSELDSDPGSALHAPNDVVVGLFDRGH"
     gene            1532..2995
                     /gene="rny"
                     /locus_tag="VGD55_06260"
     CDS             1532..2995
                     /gene="rny"
                     /locus_tag="VGD55_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY0869980.1"
                     /translation="MSTAVWVGLVIVISVVAVAASVAVILVLRRSAASDARAAGGSAL
                     DATYSELAELRAQASSDAAETRRRAQEQADLVDESRRAHEAEVQGLKAELRELRSDLE
                     RRERRLAERELRIDTQQREVEVRERDVAKLDADVTKRRDELEDVAEERRLILERTAGL
                     TAEAAKAELVTAMENQAKREAAIVVREIERTATEDADRRARNIVTLAIQRVASEQTAE
                     SVVSVLHLPSDDMKGRIIGREGRNIRAFETVTGVNLIIDDTPEAVLLSCFDPVRREVG
                     RITLEKLVLDGRIHPHRIEEIYERSVAEVEQLCARAGEDALVEVGITSIHPELVALLG
                     QLRYRTSYGQNVLKHLIESAHLAGIMASELRLDATLLKRCALLHDIGKALTHEAQGSH
                     AIIGAEIARKYGENDDVVHAIEAHHNEVEVRTIEAVLTQAADAISGSRPGARRESLES
                     YVQRLERLEQIASSYDGVEKVFAMQAGREVRVMVLPD"
     gene            3150..4121
                     /locus_tag="VGD55_06265"
     CDS             3150..4121
                     /locus_tag="VGD55_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0869981.1"
                     /translation="MKVLFIGGTGIISSACSRRVIATGSELTILNRGTTTTREVPEGA
                     EVVVADIRDPASVRAALGNRSFDVVADFIAFTPEHVQTDLDLFRDRVGQYVFISSASA
                     YQTPPARLPIVESTPLRNPYWQYSRDKIACEDLLVSAYRADGFPATIVRPSHTYDRTL
                     VPLMGGWADIERMRRGQPVVVHGDGTSLWTMTHHDDFAKAFVGLLGHPQAIGDSFQIT
                     SDEVLTWNQIYNIMADAAGVQATLVHVASDTIAAHDAELGASLLGDRTHSVIFDNTKV
                     KSLVPDYVAAIPFVTGAREIVEWYDADSSRRVVGTDLDALFDKLIAR"
     gene            complement(4127..4828)
                     /locus_tag="VGD55_06270"
     CDS             complement(4127..4828)
                     /locus_tag="VGD55_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0869982.1"
                     /translation="MTSSAKSVRIVVADDHEVVRAGFAELLDTRTDFTVVGTAADGAE
                     AVRQCQELQPDVVLMDVRMPGMDGIEATRQLANSGQSGPRVIILTTFDLDEYVYEALR
                     AGASGFLLKDVTAERLFDAVRVVADGEALLAPGVTRRLIAEFAALRRRTEADAGGELA
                     PASLDVLTARETQVLRLVAEGLSNAEIAVRLVVNEETVKTHVSRVLRKLGLRDRTQAV
                     VLAYESGLVVPHAQR"
     gene            complement(4825..>5476)
                     /locus_tag="VGD55_06275"
     CDS             complement(4825..>5476)
                     /locus_tag="VGD55_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0869983.1"
                     /translation="SFEHAARGERARIARELHDVVAHHISMVAVQAETARLTTPGMPA
                     AGAQRLSAIGDTARTALTEMRRLLGVLREDADRDLADNRRPQPGLKQLNELLDDSREA
                     SGSATRLIVHGSPVALDPGVELAAYRIAQEALTNSRRHAPGAAVDVELRYTDEILCLR
                     VRDNGPGPPFATPDGAHGLLGMGERAAAVGGQFRFGAAVGGGFLVEATLPATAVSP"
BASE COUNT          973 a         1790 c         1819 g          894 t
ORIGIN      
        1 atcgacgttc gtcggatcga gaccttgaaa gatgggcaag aagcggtcgg taaccgcact
       61 cgtgcgaagg ttgtgaagaa taagtgcgct gcccccttcc gccaagcaga attcgatatc
      121 atatataatc agggcataag ccgcgaaggc agccttatcg acatgggtgt cgacaacggc
      181 atcgtccgta agtccggtgc ttggtacacg tacgaaggtg accagctcgg tcagggcaag
      241 gagaactcgc gcacgttcct caaggacaac cccgacctcg ctgacgagat cgcgaagaag
      301 atcaaggaga agctcggcat cggtccacgt ctcgatgtcg acgtcccgat cgatctcacg
      361 gtccccgtac ctatcgacct gtgagcggga gcttcggcaa tcgtcgagga gcgggttcga
      421 caaagcgagc gcggcccggt ggccatgagg acagttccga gctgtcaggc gacccgacgg
      481 aaccgatggc ggatccgaca tcagtggctc gctcgatcgg actcgacctg ctcgacgcgt
      541 cgccgaagac ccgggctcag cttgccgagg ccatgcgccg acgaggcgtg cccgacgccg
      601 cggcgaccga ggtactcgac cggttcgacg aggtcggcct gatcgacgac gccgccttcg
      661 ccgacgcgtg ggtgacatcc cggcaagccg gccgcgggct cgcgccgcgg gcgctggcga
      721 acgaactgcg ccagcgcgga gtcccgcaag agctcatcgc tgacgcgctg gggcgggtcg
      781 acagcgacga cgtcgagtcg gcggcccgcg acctggtgac ccgccgcgcc aggagctctg
      841 ccggcctgcc gacccaagtc cggatgaggc ggctggttgc gatgctgaac cggaagggct
      901 actcgggaga gctagcggtt cgggtggtcc gcgaggtgct cgcggtcgac gacagagacc
      961 ccgacagcga ccccgacaga gaccccgagg gcggtcccgg cagcgagctc gacagcgatc
     1021 cgggaagcgc cctccacgcc ccaaatgacg tggtcgtggg tctgttcgac cggggccatt
     1081 aggagctggg cggcgaggat cggcagcgag cgagaccgga tcgccgggcc gttcggccgg
     1141 tcgcttgacc cgctgatggg gcacacatag cctcgttaaa cagtgaggag ttccccgccg
     1201 gtggcagggc tcgcgacgca ccacagtgac gaccttgaac cacagtgacg gcttacggct
     1261 acctgacgac atatgacacg gctcgaatga gttatctgag acgacttcaa cccacccggc
     1321 atgccggggc ggtttctgta ggaacgtgag gcgcaacccg gtgaacccgc cggtcagcgc
     1381 cttgcgggcg ccacgtgagg cctcgacaaa gaggccagca cctcggcgtc cgcgcatcgt
     1441 gtcgcgtttc tcgtgggcgg tttgcgtcgt tgctcggttc cgtgtcgcaa ggcagctcct
     1501 gcgctgcgac tgacatggaa ggagcccgcg aatgtcgacg gcggtctggg tcgggctcgt
     1561 gatcgtgatc tcggtggtcg cggtcgccgc gagcgtcgcc gtcatcctgg tcttgcgcag
     1621 gtctgccgcc tcagacgcgc gcgctgctgg agggagcgcc ctcgacgcga cctattccga
     1681 gcttgccgag ctgcgggccc aagcctcctc ggacgctgcc gagacccggc gccgagcgca
     1741 ggagcaggcc gacttggtcg acgagtcacg ccgggctcac gaagctgaag tccagggtct
     1801 gaaggccgag ctgcgcgagt tgcggtcaga ccttgagcgc cgcgaacgcc gcctggccga
     1861 gcgggagctc cgcatcgata ctcagcagcg cgaggtcgag gtgcgcgagc gagatgttgc
     1921 gaagctcgac gccgatgtca cgaagcggcg cgacgagctc gaagacgtgg ccgaggaacg
     1981 gcgcctgatc ctcgagcgca ctgccggact caccgccgaa gccgccaagg ccgaactcgt
     2041 gaccgccatg gagaaccagg ccaagcgcga ggccgcgatc gtcgtccgcg agatcgagcg
     2101 caccgccacc gaagacgccg accgccgcgc gcgcaacatc gtgacccttg ccatccagcg
     2161 ggtcgcgagc gagcagaccg cagagtcggt ggtctcggtg ttgcatttgc ccagcgacga
     2221 catgaaaggc cgcatcatcg gccgcgaggg tcgcaacatc cgcgccttcg aaaccgtgac
     2281 tggcgtgaac ctgatcatcg acgacacacc tgaggccgtg cttctctcgt gttttgatcc
     2341 ggtgcgtcgc gaggtcggcc ggatcaccct ggagaagctc gtgctcgacg ggcggatcca
     2401 cccgcaccgg atcgaggaga tctacgagcg cagcgtcgcc gaggtcgagc agctgtgcgc
     2461 gcgggcgggc gaagacgccc tcgtcgaggt cggcatcacc tcgattcatc ccgaactggt
     2521 ggcgctcctt ggccagcttc ggtaccgcac ctcgtatggc cagaatgtgc tgaagcacct
     2581 catcgaatct gcccaccttg ccggcatcat ggccagcgaa ctgcgactcg acgcgacgtt
     2641 gttgaagcgc tgcgcgttgc tgcacgacat cggcaaggcg ctgacccacg aagcgcaggg
     2701 cagccacgcg atcatcggcg ccgagatcgc acgcaagtac ggcgagaacg acgacgtcgt
     2761 gcacgcgatc gaggctcacc acaacgaggt cgaggtccgc acgatcgagg ctgtgctcac
     2821 gcaggcagcc gacgcgatca gcggcagtcg gccgggtgcg cggcgcgaat ccctggagtc
     2881 gtacgtgcag cgcctcgagc ggctcgagca gattgcgagc tcgtacgacg gagtcgagaa
     2941 ggtgttcgcc atgcaggccg gacgcgaggt gcgcgtgatg gtgctgcccg attagatcga
     3001 cgacattcag gcccaggtga tcgcgcgcga cattgccaag cagatcgaag aagagcttgt
     3061 gtaccccggt cagatccggg tcacggttgt tcgcgagtcg cgcgccaccg aacttgcgca
     3121 ctgagccaat cactgaggaa aggtgtcacg tgaaggtctt gttcatcggc ggtaccggca
     3181 tcatcagctc ggcgtgttcg cgccgggtga tcgcgacggg ctccgagctc acaatcctca
     3241 atcgggggac gacgaccacg cgcgaggtgc ccgagggcgc cgaggtggtc gtcgccgaca
     3301 ttcgcgatcc cgcgtccgtg cgtgccgcac tgggcaaccg cagcttcgac gtagttgccg
     3361 acttcattgc attcacgccc gagcacgtgc agaccgatct tgacctgttc cgcgacagag
     3421 tcggccagta cgtcttcatc agctcggcgt cggcctacca gaccccaccg gcccggctgc
     3481 cgatcgtcga gtcgacgccg ctgcgcaacc cctactggca gtactcacgc gacaagatcg
     3541 cgtgcgaaga cctgcttgtt tctgcgtatc gcgccgacgg atttccggcc acgatcgtgc
     3601 gaccttcgca cacctacgac cgcacgctgg tgccgctgat gggcggttgg gccgacatcg
     3661 agcgcatgcg ccgcggccag ccagtcgtcg tccacggtga tggcacgtcg ctttggacca
     3721 tgacacacca tgacgatttc gcgaaagcat tcgtcggtct gctcggacat ccgcaggcga
     3781 tcggcgacag cttccagatc acctccgatg aggtgctgac gtggaatcag atttacaaca
     3841 tcatggcgga cgccgcgggc gtgcaagcaa cgctcgtgca cgttgcttcc gacacgattg
     3901 ccgcccacga cgccgaactc ggtgcgtcgc tgctcggcga tcgcacccac tcggtgatct
     3961 tcgacaacac caaggtgaag tcgctggtgc ccgactacgt ggcggcgatt ccatttgtca
     4021 cgggcgcgcg agagatcgtc gagtggtatg acgctgattc gtcccggcgt gtggttggca
     4081 ccgatctcga tgcgctgttc gacaagctaa tcgcgcgctg agttttctac cgttgcgcat
     4141 gcgggacgac gagtcccgat tcgtaggcca gcacgaccgc ctgggtgcga tcacgcagcc
     4201 cgagtttgcg caggacccga ctcacgtgtg tcttgactgt ctcctcgttc acaacaagcc
     4261 tgaccgcgat ctccgcgttc gaaagaccct cggcgaccag ccgcaacacc tgagtctcgc
     4321 gcgccgtgag cacgtcaaga cttgctggcg cgagttcgcc accggcgtcg gcctccgtcc
     4381 tgcggcgtaa cgccgcgaac tcggcgatga gacggcgtgt gacgccgggc gccagcaggg
     4441 cttcgccgtc tgccactacc cgaaccgcgt cgaaaagcct ttcagcggtg acatctttga
     4501 gcaagaagcc gctggcgccg gcgcgcaacg cctcgtagac gtattcgtcc agatcgaacg
     4561 tggtcaagat gatgacccgc ggcccgctct ggcccgagtt cgcaagctgc cgggtcgctt
     4621 cgatgccgtc catgccaggc atccgtacgt ccatcagcac gacatctggc tgcaactcct
     4681 ggcactggcg cactgcttcg gcgccgtcgg cggccgtgcc gaccacggtg aaatcggttc
     4741 tggtgtcgag caattcggcg aacccggcgc gcacgacctc gtgatcgtcg gcgacgacga
     4801 tgcgcactga cttcgcactc gatgtcatgg gctcaccgcg gtcgcgggca gcgttgcctc
     4861 tacgagaaac cctccgccga ccgcagcacc aaatcgaaac tggcccccga ccgccgcggc
     4921 acgttcgccc atgccgagca acccgtgcgc accgtccggt gtcgcaaacg gcggccctgg
     4981 cccgttgtcg cgaacgcgca ggcacaagat ctcgtcggtg tatcgcaatt cgacgtcgac
     5041 ggcggcgccg ggcgcgtgcc gccgcgagtt cgtcagcgcc tcctgcgcga tgcggtaagc
     5101 ggcaagttcg acgcctggat cgagagcgac cggcgagccg tgcacgatga gccgggtggc
     5161 cgacccagac gcttcgcgcg agtcgtcgag cagttcgttc agctgcttga ggcccggctg
     5221 gggtcgccga ttatctgcga ggtcgcggtc ggcgtcttcg cgcaggacgc cgagcagtcg
     5281 ccgcatctcg gtcagcgccg tgcgcgcggt gtcgccaatg gcagagagcc gttgtgctcc
     5341 cgcggcaggc atgcccggcg tggtgaggcg agcggtttct gcttgcacgg cgaccattga
     5401 gatgtggtgg gcgacgacgt cgtgcagctc gcgagcgatg cgtgcgcgtt caccgcgggc
     5461 cgcatgttcg aaggac
//
LOCUS       DASWVF010000349         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_60010, whole genome shotgun sequence.
ACCESSION   DASWVF010000349 DASWVF010000000
VERSION     DASWVF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60010"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGD55_06280"
     CDS             <1..182
                     /locus_tag="VGD55_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869984.1"
                     /translation="HRVLLERRNLKDGRDDGPCPRVGSLTGVDRAGLKLTLRLKLRLL
                     GMICHDVEAIPKPLS"
     gene            258..953
                     /locus_tag="VGD55_06285"
     CDS             258..953
                     /locus_tag="VGD55_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0869985.1"
                     /translation="MAWRERPVAAAAFADARELIFRPKFRRAIVSGLVFVAAAVMGAY
                     DDGVKAKHVVDNKLVPDHPHRLVALVAIGVCLVAGVLTVQLVGNELARLARHRGSPAA
                     ASAIRLGVQIIGYLAILVTILGLFAVKIESVLLSGAIVSVVLGLAAQQSLGNAFAGVV
                     LLVSRPFKVGDYITLRAGALGGQYDGRVTAISLMFTMMQTAEGPISFPNAAVLSSATG
                     VRDQPGGTSQLIT"
     gene            1346..2692
                     /locus_tag="VGD55_06290"
     CDS             1346..2692
                     /locus_tag="VGD55_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869986.1"
                     /translation="MQVTLAGTQLVTAAILLGSLPPPSTDPACPEPKRSDLLLFTSAD
                     GSTTAVRIDPSSGCTFAWGDSGPHSYAPAKLLWVLSALDTPPASAVASPTTVDEEPYR
                     QPLGLAFPTATHGVLLAVDCPLLPGDCELFSEVSDDSGLTWSARTPMLSTHWTQPDDP
                     PLEFGPAGLAFADASLGYAYAGSDLYKTTDGGHTWIQLAVDGQVSSVVNAGTSTWVVV
                     LHGCRDGSCTGWILDTLTPDGHLASTSTQPTLPPLPEGSDGAAVPFSLLRPDDTTAYV
                     AGFDSVEVTQNGGGTWNTHTTPCTGSHPELAGISAGDPTMLWAVCSAGMGVGFEDKQL
                     WRSTNAGSSWIGPLPLEGDGYSHEITAVSSTTAWRYGDRGNLLHTSDSGRTWQGLLLD
                     AFNTAFGSPSAFAALGTQDAWVFDPYGGYGTEERDLYITADAGRTWRVVAVLKGAR"
BASE COUNT          455 a          984 c          903 g          507 t
ORIGIN      
        1 cccaccgcgt cctgctggaa cgtcgaaacc tgaaagatgg gcgggacgac ggcccctgtc
       61 cgcgggtcgg aagcctgacc ggcgtggatc gcgcgggtct caaactgacc ctgcggctca
      121 aattgcgcct gctcgggatg atctgccatg acgtcgaggc tatcccgaag ccactaagtt
      181 gaccgtgatc tcgtgtcaca gctcgtctcg cagctcgatt cagatctcgt cccgcagctc
      241 gtctcaggga gggcaccgtg gcctggcgcg aacgaccggt agcggcggcg gctttcgccg
      301 acgcacggga gctgatcttt cgcccgaagt tccggcgcgc gatcgtgtcg gggttggtgt
      361 tcgtcgccgc ggccgtcatg ggtgcctacg acgacggcgt caaggccaag cacgtggtcg
      421 acaacaagct ggtgcccgat catccgcatc gtttggtcgc cctcgtagca atcggggtgt
      481 gcctcgtcgc tggcgtccta accgtgcaac ttgtcggcaa cgaactcgct cggctggctc
      541 gacaccgcgg cagcccggcg gcagcgtccg caatccgctt gggcgtgcag atcattggct
      601 acctagcgat tctcgtcacc atcctcggct tgttcgccgt gaagatcgag agcgtcctgc
      661 tgtcgggagc gatcgtcagc gtggtgctcg gccttgcggc gcagcagtcg cttggcaatg
      721 cgttcgcagg cgtcgtgcta ctcgtttcaa gacccttcaa ggtcggcgac tacatcacgc
      781 ttcgggctgg cgcgctcggc gggcagtacg acggccgggt gactgccatc agcttgatgt
      841 tcacgatgat gcagaccgct gaaggtccga tctcgtttcc gaacgcagcg gttctgtcat
      901 cggcaacagg agtgcgcgat caacccggcg gcacctcgca gctcatcacc tgagcgcacc
      961 acgcaacctt ccgcaggccc cgcgcgtcgc cgttcgtggg aggttcctcg tgcttgaccg
     1021 agcgcacgcg tccgctgcac tgatcggcgt gattttcgtc gcggtgttgt cgtcgtgcgc
     1081 agcgcctcct gttcgtagcg ctcagtcggc gccgtcatcg ctggcaccgt catccctggc
     1141 gccgtcatcc ctggcaccgt cacccctggc accgtcaccc ctggcaccac catccccgca
     1201 ggcaccgtcg tccggctgcc cagcaactga ccaatggccg gcggccgcga ccgtgccgcc
     1261 aacggacgtc cggctctttg tctcggcgtc cccaacagca gccgtcgtgt gcgagtactc
     1321 accggccgaa ccccgtactc ccgccgtgca ggtgacattg gccggcacgc aacttgtcac
     1381 tgcggccatc ctgctcggct cattgccgcc tccgagcacc gatccagcgt gcccggaacc
     1441 gaagcgctcc gatctgttgc tgttcacctc cgcggacggc agcacgaccg cagtgcggat
     1501 cgaccccagc agtgggtgca ccttcgcgtg gggcgactcc ggcccgcact cctacgcgcc
     1561 cgcgaagctc ctgtgggtcc tctcggcgct ggatacaccg ccggcatccg ccgtggcgag
     1621 cccgacgacg gttgatgaag agccgtaccg tcagccgctg gggcttgcgt tccccacggc
     1681 cacgcacggt gtcttgctgg cggtcgattg ccccttgctg ccaggggatt gcgaactctt
     1741 cagcgaggtc agcgacgaca gcgggctgac gtggtcggca cgcacaccga tgctgtcgac
     1801 gcactggacg cagcccgacg acccacccct cgagttcgga cccgcggggc tcgcctttgc
     1861 cgatgcgtcg ctcgggtacg cctacgccgg atctgaccta tacaagacga ccgacggcgg
     1921 ccacacgtgg attcagctcg cggtcgacgg gcaggtgagc tcggtcgtca atgccggcac
     1981 atccacgtgg gtggtggtgc tgcatggatg ccgcgacggc agctgcaccg gctggatcct
     2041 cgacacgctc acacccgatg gacacctcgc cagcacgtcg acccaaccca cattgccgcc
     2101 gttacccgaa ggcagtgacg gtgccgctgt gccgttcagc ctgctgcgac ccgacgacac
     2161 caccgcctac gtggctggct tcgacagcgt ggaagtcacc cagaacggtg gcgggacttg
     2221 gaacacacat acgacgccgt gcactggctc gcatcccgaa ctcgcgggca tcagcgcagg
     2281 agacccgacc atgctgtggg cagtgtgttc ggccggcatg ggcgtcggct ttgaggacaa
     2341 gcagctgtgg cgcagcacga atgctggttc gagctggatc ggcccgttgc cgttggaggg
     2401 cgatggctac tctcacgaga tcacagcggt gagttcgacg accgcatggc ggtacggtga
     2461 tcgtggaaac ctcctccaca ccagcgacag cggccgaacc tggcaggggc tcctgctaga
     2521 cgctttcaac accgcgttcg gatcgcccag cgccttcgcg gccctcggca cgcaggatgc
     2581 gtgggtcttc gatccatacg gtggttacgg aacagaagag cgggacctct acatcaccgc
     2641 cgatgccggt cgaacctggc gcgtggtggc cgtcctgaag ggcgcacggt agcgacgcgg
     2701 caacccaccg acgcctagca ccccgccgtc cgcaaggatg aagccctatg cctcgactca
     2761 tggtgatcgt cgccagcacc cgccccggtc gcgttgggcg caaggttgcc gactggttcg
     2821 tcgaggaggc cgctgccaat gcaatgttc
//
LOCUS       DASWVF010000350         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_9524, whole genome shotgun sequence.
ACCESSION   DASWVF010000350 DASWVF010000000
VERSION     DASWVF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9524"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGD55_06295"
     CDS             complement(<1..569)
                     /locus_tag="VGD55_06295"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0869987.1"
                     /translation="MDEVFKALADPTRRELLDELFREDGQTLRALEERFAMTRFGVMK
                     HLKVLQDAGLVVTRRRGREKLHFLNPVPIRLVHDRWVSKYAQPWTAALSDLKHRMETS
                     MEKVFEIYIRTTPERLWEAITDTDIRSKYQFGMRATSDWSPGSRYEMRAGGSDGELGH
                     LIGEGENLEVDPPRRLVQSMVALWGDDVK"
     gene            779..>1527
                     /locus_tag="VGD55_06300"
     CDS             779..>1527
                     /locus_tag="VGD55_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869988.1"
                     /translation="MKTALRRLWRDSTTLQIGVHHERSVVVAGVGDQVGRLLAAFDGA
                     HDRSALRARARSLGLDASIVDRLVSMLTEAAVLDDAATDTHPLAVLPRIERDRLAPDV
                     ASMSLVSGELDGGLSTVSRRQLATVCVVGAGRVGGAVATLLGAAGIGHLFVDDAATCR
                     PADCGPAGAALGDTGSTRAQATHAAIHRISQITRTTALTPAQHYDVVVLATGDAPEMG
                     ALNELVRSGVPHLRVVVREATAVIGPFVLPG"
BASE COUNT          241 a          494 c          507 g          285 t
ORIGIN      
        1 ctttttacgt cgtcccccca caacgcgacc atgctctgaa ccaatcggcg cggtgggtcg
       61 acctcgaggt tctcgccttc gccgatcagg tggcccagct cgccatcact gccgccggct
      121 cgcatctcat agcgagagcc agggctccag tcggacgtcg cgcgcatgcc gaactggtac
      181 ttgctgcgga tgtcggtgtc agtgatcgcc tcccacaacc gctccggagt ggtgcggatg
      241 tagatctcga aaactttctc catcgaagtc tccattcggt gttttaggtc gctgagcgcg
      301 gccgtccatg gctgtgcgta cttgctcacc cagcggtcgt ggacgagccg gatcggtacg
      361 gggttgagga agtggagctt ctctcggccg cggcgtcggg tgacgacgag tcccgcgtcc
      421 tgcagaacct tgaggtgctt catgacgccg aaacgtgtca tggcgaagcg ctcctcgagt
      481 gcgcgcaacg tctgaccgtc ctcgcggaag agctcgtcga ggagctcccg tcgcgtggga
      541 tccgccagtg ccttgaagac ctcgtccacg tcacgaaggt aggtgaccaa atagtcacat
      601 gtcaaggtcg tctttcccac aaccatcgcg gcgtctttcc cacaagcctc atggcgctgg
      661 caccggcgcc tgtggacggt cgcgcagtgg cggctggcct cgatgacacc ctgaggcctt
      721 gcctgcgatc tgcgggtgcg cgagataacg agccgaagga tcaccgttgc gtccggtcgt
      781 gaagacagcg ttgcgacggc tgtggcgtga ctcgacgacg ctgcagatcg gcgtccatca
      841 cgaacgaagt gtcgtggtcg ccggcgtggg tgaccaggtg ggccggctgt tagccgcatt
      901 cgacggcgcg cacgatcgat cggcattgcg agctcgggcc cgctcgttag gtcttgacgc
      961 ctcgatcgtg gaccggctcg tgtcgatgtt gaccgaagcg gcggtcctcg acgatgcagc
     1021 cacagacacc cacccgctcg ccgtgctccc ccgcattgaa cgcgaccggc tcgcacccga
     1081 tgtcgcatcg atgtcgctgg tttcaggaga gctcgacggc gggctctcca ccgtctcgag
     1141 gcggcaactc gccaccgtgt gcgtcgttgg cgccgggcgg gtggggggcg ccgttgcgac
     1201 actgctaggt gccgcgggga tcggtcatct gttcgtcgat gacgccgcga cgtgccgacc
     1261 ggcagactgc gggccggctg gggcggcgct tggcgatacc ggttcgaccc gcgcgcaagc
     1321 cacgcacgcg gcgatccacc ggatcagcca gatcactcgc acgacagcac tcacgccagc
     1381 tcagcactac gacgtcgtgg tcttggccac cggcgacgca cccgaaatgg gtgcgcttaa
     1441 cgagttggtg cgatccggtg tgccacacct gcgggtggtc gtgcgggaag cgacagcggt
     1501 catcggcccg ttcgtgctgc ctgggag
//
LOCUS       DASWVF010000351         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_34838, whole genome shotgun sequence.
ACCESSION   DASWVF010000351 DASWVF010000000
VERSION     DASWVF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34838"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGD55_06305"
     CDS             <1..515
                     /locus_tag="VGD55_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0869989.1"
                     /translation="NVVSFQENLRAARLGNFPSINASAAYGTNSTDISGGTYRSSGSA
                     GLTLTIPVYDRGVTRAQTAQAQGQLDNAVAQLVAAQQGVQLNVRTALFNLVAAYAALD
                     QTNAELTQAQQVLLSTEAQYRAGVTTLPLLLNAQVGITTALTDEVNAAYAVRQAEQAL
                     LYAEGANAAG"
     gene            528..>2009
                     /locus_tag="VGD55_06310"
     CDS             528..>2009
                     /locus_tag="VGD55_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0869990.1"
                     /translation="MISNRRLAAAVGAVLVLCACTRGADGPGTAAAGSGGNSWTHPGR
                     LVMSAASDPKNLNPMIDSSQPTLELSMFLFSYTVRYDQNSQPVPDAVSEVPTIANGDV
                     ARDGLTLRYKLRHDIFWHDGVQLTCRDLRFTWQAVMNPHNNVQTTVGYDAIKDIDCRD
                     PFIAIVHMKRVYASFLQQLWSVNGNAPIMPEHLLAKYNDAKGSFNNAPFQAAPVGSGP
                     FRFVAWDRGSDVRLAAYDRYFRGRPGLNEVVYRIIPDGNTLVTQLQTHEVDLGFNLPA
                     TSWDRARSIPGTTPITPVVYSWVHIDFNLRRPIFSDVRVRRALTYALDRPSLLVKVQH
                     GLGELCDTFINPTLSPQAVNRDVMKYPYDPAKARTLLDAAGWRVGPGGVRTRNGQRLA
                     FTFSTQIESNTGQAMEVQAQSYWRAIGAEVTVKNYPTSQFFANDLTGILTSGKYDVAT
                     YSWAGAADIDQSAIYSAHYMAPHGQNFFFWNNALATAKMDDANE"
BASE COUNT          370 a          688 c          624 g          327 t
ORIGIN      
        1 agaacgtcgt ctcgtttcaa gagaacctgc gcgcagcgcg gctcggcaac ttcccctcga
       61 tcaatgccag cgccgcctac ggcaccaact cgaccgatat cagcggcggc acctaccgca
      121 gtagcggctc ggcgggtctg acgctgacca ttccagtcta tgaccgcggc gtcacgcgcg
      181 cgcagacggc tcaggcgcag ggccagctcg acaacgccgt tgcgcaactc gtcgccgcgc
      241 agcagggtgt ccagctcaac gtccgcacgg cgttgttcaa tttggtcgcc gcgtatgcgg
      301 cgctcgacca gaccaacgcc gaactcaccc aggcgcagca agtgctgctg tcgaccgaag
      361 cgcagtaccg ggcgggcgtc acgacgctgc cgctgctgct caatgcccag gtcgggatca
      421 ccaccgcact cacggacgag gtcaacgcgg cgtacgcggt ccggcaggcc gaacaggcgc
      481 tcctgtatgc ggagggcgcg aacgccgccg gctgaactcc ccgtcgcatg atatcgaacc
      541 gaaggctcgc cgcggcggtc ggcgccgtgc tggtgctgtg cgcctgcacg cggggggccg
      601 acgggccggg gacggccgct gcgggcagcg gcggaaattc gtggacccac ccgggccggc
      661 tggtcatgtc ggcggcctcc gacccgaaga acctcaaccc gatgatcgac tcgtcgcagc
      721 cgacgctcga gctctcgatg ttcctgtttt cgtacacggt gcggtacgac cagaactcgc
      781 agcccgtccc cgacgccgtc agcgaggtac cgacgatcgc gaacggcgac gtcgcgcgcg
      841 atggactcac gctgcgctac aagctgcgtc acgacatttt ttggcacgac ggcgtacaac
      901 tgacctgtcg cgacctgcgc ttcacctggc aagcggtgat gaacccgcac aacaacgtcc
      961 agacgacggt cggctacgat gcgatcaagg acatcgattg ccgcgacccc ttcatcgcga
     1021 tcgtgcacat gaagcgcgtg tacgcatcgt ttctccagca gctgtggagc gtcaacggga
     1081 acgcgccgat catgcccgag cacctgctcg ccaagtacaa cgatgccaaa ggatcgttca
     1141 acaatgcgcc gtttcaggcg gcgccggtcg gcagcggccc gtttcggttc gtggcatggg
     1201 accgcggcag cgacgtgcgg ctggcggctt acgatcggta ctttcgcggt cgccccggtc
     1261 tcaacgaggt cgtgtaccgg atcatccccg acggcaacac gctggtgacg cagctgcaga
     1321 cccacgaggt cgatctgggt ttcaacttgc cggccacttc atgggaccgc gcgcgttcga
     1381 taccgggcac gacgccgatc acgcccgtcg tctattcctg ggtgcacatc gacttcaacc
     1441 tgcgccggcc gatcttctca gacgtgcgcg ttcgccgcgc gctgacctac gcgctcgacc
     1501 ggccgtcgct gctcgtcaag gtgcagcacg gcctcggtga gctctgcgac accttcatca
     1561 acccgacgct ctcaccgcaa gccgtgaacc gtgacgtgat gaagtatccg tacgatcccg
     1621 ccaaagcgcg cacgctgctc gatgccgcgg gctggcgggt gggaccgggc ggcgtccgga
     1681 cccgcaacgg tcagcgcctt gcattcacct tttcgaccca gatcgagtcg aacaccggac
     1741 aagcgatgga ggtgcaggcg cagagctatt ggcgcgcgat cggcgcggag gtgacggtca
     1801 agaactatcc gacctcgcaa tttttcgcca acgacctgac cgggatcttg accagcggaa
     1861 aatacgacgt cgccacctat tcgtgggccg gcgcggccga catcgaccag agcgcgatct
     1921 attcggcaca ctacatggcc ccgcacggac agaacttctt cttttggaac aacgcgctgg
     1981 ccaccgcgaa gatggacgat gccaatgag
//
LOCUS       DASWVF010000352         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_493454, whole genome shotgun sequence.
ACCESSION   DASWVF010000352 DASWVF010000000
VERSION     DASWVF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493454"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..1240
                     /locus_tag="VGD55_06315"
     CDS             467..1240
                     /locus_tag="VGD55_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0869991.1"
                     /translation="MGAPLTEAAISKLREMIISGKYPSGAKLPPEPQLAAELGLSRNT
                     VREAVRALSTARVLDVRRGDGTYVTSLRPELLLEGIAFAVDLMQTELSLELLQVRRIL
                     EPAVTALAAANIDENALVHLEKTLIRMQESASNHAELVHYDADFHAQVAAAAGNDTLA
                     SMLNGISSRTLRARVWRGIIEEEAIARTISEHARILEALRAGDVALAEAAALTHVCTT
                     EASLRRILAESVEAPTKSHQPAKAHAPAKAHSATAKRKP"
     gene            complement(1237..>1626)
                     /locus_tag="VGD55_06320"
     CDS             complement(1237..>1626)
                     /locus_tag="VGD55_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY0869992.1"
                     /translation="GVDSQWRSACAAAVPLVLVDGEPDPLTAAQLDATDAVVTGCRVA
                     IAATGTIVLDGEPDQGRRVLTLVPDHHVVIVFASQVVSSVPESLGRLDPTRPLTFISG
                     PSATSDIEFKRVEGVHGPRVLDVIVVL"
BASE COUNT          315 a          536 c          510 g          265 t
ORIGIN      
        1 atcctcagct gacatgtcga gtgacagatg ctcattcgtg tcgctgcgga gcgcttgatc
       61 ggcactcgag acactcatta gacatccagt gtttgggtct ttatagcggc tctcgatgtg
      121 gctgtaaagc gtccgatacc acaccgttac caagctggca gtgaggcaat tgcgctctcc
      181 cagcgcggaa gtttagcgag agtggccact atgtcgcatt tgcccgcgcg accccgcgtg
      241 ggcgtcagag atccgacgac ttcttccgcc aagaagttga tcacgcccgg cagcggaagg
      301 ccccccgtag gcaggcgtgg gcgcctgtct aggccactcc ccggccgctc aggcaggctg
      361 gcgtcgcgga aattcggcgg catactgacg tccgatgtct gcgggtatcg atgtcgacca
      421 atccgctggc tagactgcgt cgacaggccg acaagatgag ggcatcgtgg gtgcaccact
      481 gaccgaagcg gcgatcagca agcttcgcga aatgatcatc tcgggcaagt acccgtcagg
      541 cgccaagctt cccccggaac cgcagttggc cgccgaactg ggcttgtcgc gcaacactgt
      601 gcgcgaggcc gtgcgggcgc tgtcgactgc gcgagtgctc gacgttcgac gcggcgacgg
      661 aacctacgtc accagcctgc gccccgaact gttgcttgag ggcatcgcct tcgccgtcga
      721 cctcatgcag accgagttgt cgctggagtt gctgcaggtt cgccggatct tggagcccgc
      781 cgtcaccgcg ctcgcagcgg ccaacatcga cgagaacgcg ctggtacatc tggagaaaac
      841 gctcatccgg atgcaggaaa gtgcctcgaa ccacgcggaa ctcgtgcact acgacgctga
      901 tttccacgcg caagttgccg ctgccgccgg aaacgacacc ctcgcgtcga tgctgaacgg
      961 aatctccagc cgtaccttga gagctcgagt ctggcgcggc atcatcgagg aagaagcgat
     1021 tgcgcgcacc atctccgaac acgcgcggat cctcgaggcg ctgcgcgccg gagacgtggc
     1081 gctcgccgaa gccgctgcgc tcactcacgt ctgcacgacc gaggcatcgc tgcgacgaat
     1141 tctcgccgag tcggtcgagg ccccgacaaa gagccatcaa cccgcgaagg cgcatgcgcc
     1201 ggcgaaagct cattccgcta ccgcgaaacg aaagccttaa agcaccacga tcacgtcgag
     1261 gacacggggg ccgtgcacgc cttcgacccg cttgaactcg atgtcgctcg tcgcgctcgg
     1321 gccactgatg aacgtcagcg gacgcgtcgg gtcaaggcgg cccaacgact cgggcacgct
     1381 gctcaccacc tgcgaagcga acacaatcac gacatggtgg tcgggcacga gggtcagcac
     1441 ccggcggccc tggtcgggct cgccatcgag cacgatcgtg ccggtcgcag cgatcgcaac
     1501 gcggcagccg gtgacgacgg cgtccgtcgc gtcgagttgt gcagcggtca acggatcggg
     1561 ctcaccatcg acaagcacca gcgggacggc ggcggcgcat gcactgcgcc actgcgagtc
     1621 gacacc
//
LOCUS       DASWVF010000353         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_366012, whole genome shotgun sequence.
ACCESSION   DASWVF010000353 DASWVF010000000
VERSION     DASWVF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366012"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGD55_06325"
     CDS             <1..242
                     /locus_tag="VGD55_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0869993.1"
                     /translation="SLAWLLGRAGVTSVIVGARNDDQLADNLKAADLVLSADERKRLD
                     DVSAMPLLYPHWHQAKTSGDRLGAPELTLLKPHLK"
     gene            283..477
                     /locus_tag="VGD55_06330"
     CDS             283..477
                     /locus_tag="VGD55_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869994.1"
                     /translation="MSENDDISLDAPRFDRDRDGDLRDTDEEQGDEIELDDLFDLDET
                     EAREVDADVDGDAGDEPRLD"
     gene            complement(488..1165)
                     /gene="merB"
                     /locus_tag="VGD55_06335"
     CDS             complement(488..1165)
                     /gene="merB"
                     /locus_tag="VGD55_06335"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEY0869995.1"
                     /translation="MAPHSEADTEALRVAVYQLFTATATAPSTNDLVAITGQPENEVR
                     SGLGELATARHLVLDAAGQIVMAHPFASIPLGFSVMGRHNLWWGGCAWDSFAMPHVLP
                     DEPDVLVATRCPNCDRPHAWVVERDHPPAGDQVAHFLVPTAHIWDDVVHTCSHQRLFC
                     SVQCVDSWLTKTGNTRGYVMDLATLWRLARHWYDGRLDYGYTRRDPATAAAYFREVGL
                     EGPFWGL"
     gene            1302..2102
                     /locus_tag="VGD55_06340"
     CDS             1302..2102
                     /locus_tag="VGD55_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0869996.1"
                     /translation="MATSTKPAVFIHGLWLHATSWGPWLELFRDAGYEPIAPGWPHEP
                     PSVQEARENPDAVANLGIDDVVDHYAAIINALDAPPVIIGHSFGGLMAEKLLGQGQGV
                     AGVAIDPAQIKGVLPLPLAQLRAGLPGLGNPTNIHKAVSLTQKEFRFGFGNALSEEES
                     DALFEKWTIPSPARPLFQAAAANFALHSEAKVNTDNNTRGPLLLISGLEDHTVPDVVT
                     RSTLKQYRDSTSVTELKQFEGRGHSLTVDNGWKEVANTVLDWLKTNGV"
BASE COUNT          413 a          733 c          754 g          357 t
ORIGIN      
        1 tgtcactcgc ttggttgctc ggccgcgcgg gggtcacgtc ggtgatcgtc ggcgcacgca
       61 acgacgacca actcgccgac aacctcaagg ccgccgacct ggtgctgtcg gccgatgagc
      121 gcaagcggct cgacgacgtg agcgcgatgc cgctgctgta cccgcactgg catcaggcga
      181 agacatcggg cgatcgcctc ggcgcgccgg agctgaccct gcttaagcca cacctgaagt
      241 gatctcgtgc cccctatcgg ccgcctcgag gatgctgagt acatgagcga gaacgacgac
      301 atttcactcg acgcgccgcg ctttgatcgc gatcgtgacg gtgacttgcg cgacaccgac
      361 gaggagcagg gcgacgaaat agagctcgac gatctgttcg atctcgacga gaccgaagcg
      421 cgagaagtag acgcggacgt cgatggcgac gccggcgacg agccccgcct cgactgatcg
      481 gcctctccta cagaccccag aacgggcctt cgagtccgac ctctcggaag tacgccgcag
      541 cggtcgccgg atcccttcgg gtgtacccgt agtcgaggcg gccgtcgtac caatgccggg
      601 caagacgcca cagggtggcg aggtccatga cgtagccacg ggtgttgccg gtcttcgtga
      661 gccaagaatc gacgcactgc accgagcaga acagccgctg gtgagaacac gtgtgcacca
      721 cgtcgtccca gatatgcgcg gtgggcacca ggaagtgggc aacctggtca ccggccggtg
      781 ggtggtcgcg ttcgacgacc cacgcatgcg gccgatcgca gttcgggcag cgcgtcgcga
      841 ccaagacgtc gggctcgtcg ggcaacacat gcggcatggc gaacgagtcc caggcgcagc
      901 cgccccacca caggttgtgg cggcccatca ccgagaaccc gagcggtatc gaggcgaacg
      961 ggtgtgccat gacgatctgg cccgcggcgt caagcacgag gtggcgcgcg gtcgccaact
     1021 cgccaagacc tgagcgcacc tcgttctcgg gctggcccgt gatcgcgacg agatcgttgg
     1081 tcgacggcgc agtcgcggtg gcggtgaaca gttggtaaac ggcgacgcgc aacgcctcgg
     1141 tatccgcctc ggaatgtggg gccacggcga cagagtagcg agctgggctt tacacagctg
     1201 tgggcgactg ttggcttttc gggcgtgccc tgacaggctc ggaagtgccc ggtgactgat
     1261 gcccggtgcg cgtgagcacg agtcgacacg acggaggacg catggctact tcgaccaaac
     1321 ctgcggtatt catccacggc ttgtggctcc acgccaccag ctgggggccg tggctcgagc
     1381 tgttcaggga cgccggctac gagccgatcg ctccgggctg gccgcacgag ccgccgtccg
     1441 tccaagaagc acgtgagaac cccgacgcgg tcgccaacct cggcatcgac gacgtggtcg
     1501 accactacgc ggcgatcatc aacgcgctcg atgcgccgcc ggtgatcatc gggcactcgt
     1561 tcggtggact catggccgag aagttgctcg gccaagggca aggtgtcgcg ggcgtcgcca
     1621 tcgaccccgc gcagatcaag ggcgtgctgc cgctgccgct cgcgcagcta cgcgcgggcc
     1681 tgcccgggct cggcaacccg acgaatatcc acaaggcagt gtcgctgacc cagaaggagt
     1741 tcaggttcgg cttcggaaac gcgttgtcgg aggaagagtc cgacgcgctc ttcgagaagt
     1801 ggacgatccc ctcgccggcc cgcccgctct tccaggccgc agcagctaac tttgcgctgc
     1861 actccgaggc gaaggtcaac accgacaaca acacccgtgg cccgctgctg ctcatctcgg
     1921 ggctcgaaga ccacacggtg cccgatgtcg tcacccgatc gacgctcaag cagtaccgcg
     1981 actcgacgtc ggtgaccgag ctgaagcagt tcgaaggtcg cggtcactcg ttgacggtcg
     2041 acaacggctg gaaagaagtc gcgaacacag tgcttgactg gctgaagacc aacggggtct
     2101 gaggccaact gcgtcggcgg gtctagcccg cttcggagat atggatctcg ccatcgccgg
     2161 tgctcgccgt gatcgacgac gttgatgttg cgtcctgcgg cacggtgata tccgtgccgc
     2221 cgtcgccggt gtgggccgag accttgtacg cagtgcc
//
LOCUS       DASWVF010000354         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_314990, whole genome shotgun sequence.
ACCESSION   DASWVF010000354 DASWVF010000000
VERSION     DASWVF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314990"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGD55_06345"
     CDS             <1..714
                     /locus_tag="VGD55_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY0869997.1"
                     /translation="EVARRVLQRGVAGLRPDLAGRRVVVSAGGTREALDPVRFLGNRS
                     SGRQGYALAMTAVARGAEVTVVSANVTLPDPAGAKVVRVVSAEDMRAAVLEAATGADA
                     VVMAAAVADFRPVTVSASKIKKSHEDPPALELTRNPDVLAALARADRPGRLAQVIVGF
                     AAETDDVVANARTKLAAKGADLLVVNEVGTVKGFDVPDNAGVVLGADGSATPVPAGPK
                     AALADVVWDLVAARLPALS"
     gene            815..>1938
                     /gene="metK"
                     /locus_tag="VGD55_06350"
     CDS             815..>1938
                     /gene="metK"
                     /locus_tag="VGD55_06350"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0869998.1"
                     /translation="MSRRLFTSESVTEGHPDKIADQISDGVLDALIKDDPKSRVAVET
                     LITTGQVHVAGEVTTEAWADIPAIVREVILGIGYDSSKKGFDGASCGVSVSIGSQSPD
                     IAQGVDEGYERRHGEGVDELDRQGAGDQGLMFGYACDDTPELMPLPIALAHRLARRLS
                     EVRKSGVVPYLRPDGKTQVTIEYDGDKPVRLDTVVVSSQHAADIDIDTLLAPDIAEFV
                     VGPEIAALGIDCSGHRLLVNPTGRFEIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFS
                     GKDPSKVDRSAAYAMRWVAKNVVASGLATRCEVQVAYAIGKAHPVGLFVETYGTGTVD
                     DERIQEAVVQVFDLRPGAIIRDLDLLRPIYLQTAAYGHFGR"
BASE COUNT          298 a          652 c          666 g          322 t
ORIGIN      
        1 gaggtcgcgc gccgggttct gcagcggggc gttgccgggt tgcgtcccga tctggccggg
       61 cgcagggtgg tcgtctccgc gggcggcacc cgcgaggcac ttgatccggt gcgattcctc
      121 ggcaaccgca gctcagggcg ccaggggtac gcactcgcca tgacggccgt cgcccggggc
      181 gctgaggtca cggtcgtctc tgcaaatgtc acattgcccg atcccgccgg cgccaaagtc
      241 gtccgtgtcg tttccgccga agacatgcgg gccgccgttc tcgaggcggc aaccggcgcc
      301 gacgcggtcg tgatggctgc ggctgttgcc gacttccggc ccgtcacagt gtcggcgtcc
      361 aagatcaaga aatctcacga agacccgcca gcgctggagc tgacgcgcaa tcccgacgtg
      421 ctcgcagcac tggcccgggc tgaccggccc gggcggctcg cgcaggtcat cgtcggattc
      481 gcggcggaga ccgacgacgt ggtggcgaac gcccgcacca aactcgccgc gaagggagcc
      541 gatctgctgg tagtcaacga ggtgggcacc gtcaagggat tcgatgtccc tgacaacgcc
      601 ggtgtcgtgc tcggtgccga cggcagtgcg acgccggtgc cggccggacc gaaggccgcg
      661 ttggccgacg tcgtctggga tctcgtcgcg gcgcgactgc cagcgctatc gtgacgcttt
      721 tccgtgccgg ccgcggggtg tcctagactc gggcagtccg ttgaggcacc ttcagttgcc
      781 cgtgccgccg cgtgttacag acccagggag cgccgtgtcc cgccgcttgt tcacgtcaga
      841 gtccgtcacc gagggccatc ccgacaagat cgccgaccag atcagcgacg gtgtgctcga
      901 cgcccttatc aaggacgatc cgaagagccg cgtcgcggtc gagaccctga tcacgaccgg
      961 ccaggtgcat gtggcgggcg aagtgaccac cgaggcgtgg gccgacatcc cggcgatcgt
     1021 gcgcgaggtg attctcggca tcggctacga ctcctccaag aaggggttcg acggtgcgtc
     1081 gtgtggagtg tcggtctcga tcgggtcgca gtcacccgac atcgcgcagg gcgtcgacga
     1141 gggctacgag cgtcgccacg gcgagggcgt cgacgagctc gatcgccaag gggccggcga
     1201 ccagggtctg atgttcggct acgcgtgcga cgacacaccc gagttgatgc cgctgccgat
     1261 cgctctcgcc caccggctgg cgcgccggct ctccgaggtg cgcaagtccg gcgtcgtccc
     1321 ttatctgcgt cctgacggca agacccaggt gacgatcgag tacgacggtg ataagccggt
     1381 gcgtctcgac accgtcgtgg tttcgagtca gcatgcggcc gacatcgaca tcgacacgct
     1441 gcttgccccc gacattgccg agttcgtcgt cgggcccgag atcgcggcgc tcggcattga
     1501 ctgctcaggt catcgactgc tggtgaaccc gacgggtcgc ttcgagatcg gcggcccgat
     1561 gggcgacgcc ggcctgaccg gccgcaagat catcgtcgac acctacggcg gcatggcgcg
     1621 ccacggtggc ggtgccttct cgggcaagga cccgtcgaag gtcgaccgtt cagccgccta
     1681 cgcgatgcgc tgggttgcga agaacgtcgt cgcttccgga cttgcgactc gctgcgaggt
     1741 gcaggtcgcc tacgccatcg gaaaggccca cccggtcggg cttttcgtcg agacctacgg
     1801 caccggcaca gtcgatgacg aacgcattca agaggccgtg gtgcaggtct tcgaccttcg
     1861 cccgggcgcg atcatccgcg acctagacct gctgcgcccg atctaccttc agaccgcggc
     1921 ctacggccac ttcggccg
//
LOCUS       DASWVF010000355         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_213451, whole genome shotgun sequence.
ACCESSION   DASWVF010000355 DASWVF010000000
VERSION     DASWVF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213451"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGD55_06355"
     CDS             complement(<1..232)
                     /locus_tag="VGD55_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0869999.1"
                     /translation="MGTIDTDQLSVVGPPNGATIAAALQAPITAALTDYTKTVASQGP
                     CKGPACGSGDFQADFTTSRADSVVVSGTWTIST"
     gene            261..524
                     /locus_tag="VGD55_06360"
     CDS             261..524
                     /locus_tag="VGD55_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870000.1"
                     /translation="MSSTTTRCGAAGAVLAAALEAATAGALEIAVDAGGVDADAAPLD
                     VPAGVVVTAGETTGADDGALACLEPELQAAANVISATSGAARR"
     gene            complement(615..1889)
                     /gene="glyA"
                     /locus_tag="VGD55_06365"
     CDS             complement(615..1889)
                     /gene="glyA"
                     /locus_tag="VGD55_06365"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0870001.1"
                     /translation="MSDHLAALTATDPEVAALVRAEETLQSEKVRLIASENYVSQAVL
                     EATGSVLTNKYSEGYPTKRYYEGQQVIDQVETLAINRAKAVFGVDHANVQPYSGSPAN
                     LAVYLALANAGDTVMGMALPMGGHLTHGWSVSATGIWFHAVQYGVGRESGRVDMDEVR
                     EIALRERPKIIFCGGTAIPRTIDFPAFAEIAREVDAVLVADIAHIAGLIAGGAHPSPV
                     GHASVISTTTHKTLRGPRGAMLMCDEQYAKAIDKAVFPGLQGGPHNHTTAAIAVALHE
                     AQQPSFRDYAATIVTNAQALAENLSSRGYDLVSGGTDNHLILIDLTPQGVAGKPVAKA
                     LDRAGLVVNYNTVPYDPRKPFDPSGIRIGTPAVTSRGMGVSEMRQIGAWFDEVVAAVA
                     KNDEDTVNATTDRVEAEVRELTADFPAPGLPV"
     regulatory      complement(1901..1984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
BASE COUNT          331 a          642 c          704 g          374 t
ORIGIN      
        1 acgtcgagat cgtccaggtc cctgagacca caacagagtc ggcgcgactc gtggtgaagt
       61 cagcctggaa atcgccgctc ccacacgcag ggcctttgca tggcccttgt gacgccacag
      121 tcttcgtgta gtcggtgagc gcggccgtga tgggagcttg cagtgcagct gcgatcgttg
      181 cgccgttcgg cggcccgacg accgacagct ggtccgtgtc gatcgtgccc acgtcgcccg
      241 cgccggttcg atgtgagcgg gtgagttcga ccacgacacg ttgcggcgca gcaggcgccg
      301 tgctggctgc cgcgcttgag gcggcgacag ccggagcact agagattgcc gtcgacgccg
      361 ggggagtcga cgccgatgcc gcaccgctcg acgtccccgc tggggtggtg gtaacagcgg
      421 gtgagacgac aggtgccgac gacggtgcgc tcgcgtgttt agagcccgag ctgcaggcag
      481 ccgccaacgt cattagcgcg acaagcggtg ccgctcgcag gtagttacgg gatggcatcg
      541 gcacgggtct cctctgtcgc agtcgactgt gagacgccca gcgagcgcaa ctggttgtgc
      601 agaaacggaa ggcgctagac cgggagaccg ggtgcgggga agtcggcggt cagctcgcgc
      661 acttcagctt cgacgcgatc ggtcgtggcg ttcacggtgt cctcgtcgtt cttcgccacc
      721 gccgcgacaa cctcgtcgaa ccatgctccg atctgacgca tctcgctgac acccatgccg
      781 cgactggtca ccgctggcgt tccgatgcgg atgcctgacg ggtcgaacgg cttgcggggg
      841 tcgtacggca cggtgttgta gttgacgacg agacctgcac ggtcgagggc cttggcaacc
      901 ggcttgcccg ctactccctg gggtgtcagg tcgataagga tgaggtggtt gtcagtgccc
      961 cccgacacca agtcgtagcc gcgcgacgac aggttttccg cgagtgcttg cgcgttggtg
     1021 acgatcgtcg cggcatagtc gcgaaacgag ggttgttgcg cctcatgcaa cgcaaccgcg
     1081 atcgcggcgg tcgtgtggtt gtgcggaccg ccctgcaggc cggggaaaac ggccttgtcg
     1141 atggccttgg cgtactgctc gtcgcacatc aacatcgcgc cgcgcgggcc gcgcaacgtc
     1201 ttgtgcgtcg tcgtcgagat gacacttgcg tgtccgactg gcgacgggtg cgcaccaccg
     1261 gcgatgagtc cggcgatgtg tgcgatgtcg gcgaccagca cagcgtcgac ttcgcgagcg
     1321 atctcggcga acgctgggaa atcaatcgtg cgcgggatcg cggtacctcc gcaaaagatg
     1381 attttcggcc gctcgcgaag cgcgatctcg cgcacctcgt ccatgtcgac gcgcccgctt
     1441 tcgcgaccga cgccgtactg caccgcgtgg aaccagatgc ccgttgcgga gaccgaccaa
     1501 ccgtgcgtga ggtggccgcc catcggcaat gccatgccca tgacggtgtc gcccgcgtta
     1561 gcaagagcga ggtagaccgc gaggttggca ggggagcccg aatacggctg gacgttggcg
     1621 tggtcgacgc cgaacacggc cttcgcgcga ttgatcgcga gcgtctcgac ctgatcgatg
     1681 acctgctggc cctcgtagta ccgcttggtc gggtaaccct cggagtactt gttcgtcagt
     1741 acggagccgg tggcctcgag taccgcctgt gagacgtagt tctctgaggc gatcagccga
     1801 actttctcgg actgaagcgt ctcttcggcg cgcacgagtg cggcgacctc ggggtcggtt
     1861 gcggtcagcg cagcaaggtg atcagacacg gtgtctcctc ggtggacacc caggcgcacg
     1921 gcgtccgcgt cgatcgctcc ccggtggttg atccaccatc tatgcgccag tcgcgacgtc
     1981 ccgatcctag ccggaacggg cgtctagccc atgcactcga tgtgctcgac ctggaactgg
     2041 gcggtcgcgt c
//
LOCUS       DASWVF010000356         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_85753, whole genome shotgun sequence.
ACCESSION   DASWVF010000356 DASWVF010000000
VERSION     DASWVF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85753"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            915..1997
                     /locus_tag="VGD55_06370"
     CDS             915..1997
                     /locus_tag="VGD55_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870002.1"
                     /translation="MKDNGLFRLVGFAPTVAVCILAACQDVRVDPFAREARPDTGATT
                     SLDAGRLACPPEMIGFASVSGSTQPTTGGGTARPVTVSTLSDLVSEAGSVSPAVIVIK
                     GMVVVPPASQPLQIEVGSNKTIVGADASSGLTGGGFIIDATRNVIIRNLVISFPVGTD
                     GIAVQAAQHVWIDHCELYSDTNPGRATDYYGWLVNIKHASDFVTVSWTRFHDHFNTAQ
                     IGHSDDNSAEDLGHLTVTYHHNLFERTHSGSPRVRFGTVHVFNNDYDAVSDYAVASQD
                     QAQVRVEQNYFQTVAEPLTNTHEMTPPGAIGDILNTYSVDSGPNVLAGSGAPAFTLDA
                     LPYPFMTDSTSSVRAIVSSCAGPGRI"
     gene            complement(2004..>2271)
                     /locus_tag="VGD55_06375"
     CDS             complement(2004..>2271)
                     /locus_tag="VGD55_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870003.1"
                     /translation="GVGGLMIALHVSGNPTAGWAGTAQTGVGVAGCGTIGGTVALSRT
                     IALRGELIGGEAFRRPLASAGGPAALPWGPGFVTVLTAIEAHWF"
BASE COUNT          428 a          740 c          693 g          410 t
ORIGIN      
        1 cgtccgctta gggatttggt caagcaacgc ggcgcgacag gctgcgacgc ggacgatgta
       61 ccggtcagaa gacctggggc ggcaagtcgt gacgccggtg cccgagcgtt gaagacgacc
      121 gggacgagag aggccgggca ccctgtggcg cctggccgtc gatcagggcc aaactgaccg
      181 cgtggtgccg tccgccttga cctcaacagc cgtgtcagaa gctccgcgca cgattcgcag
      241 caacgatcca acgggactgt cgaacgtgcg cgccccacga ggccacgagc acggccgtcg
      301 gcttgcgtcg ggcggcgcct ggcgagacgc ggcgactgtc agccatgctg cgtggccgct
      361 tgcgtccggt tgcgggccgg cgagccgacc gatgtccatc gccccccctg cgccggtcga
      421 tctgcgagcg cgagtcgggg tcgcggagtc cttcaaggtg cgagaccgct tccagcacgt
      481 ccaatccgtc gccgacatgc aggctgacgg tctatgcgac tcgtctcgcc cgccggggag
      541 caatgtcggg aggcggattc agctccaccc acttgaaaag atcgtcaggt cgcgcaccgc
      601 gacggtggta gtcgataagg ccgtgcacct tgtagagcgg cccccgccaa ccacgaaggc
      661 gatgccacca tgcgctatac catcgggcac gacgcacatg gagctcgaaa cgattctctg
      721 cccccgtgcc gctgtttgtc gtgccggtcc aatcgtccac ggttggattc agttgcggcc
      781 aaagaccgaa acggctcaga tcgagcgcag gccgttccga ggacggtcga agttgccaca
      841 agacccgtgt gacatcagtc gcgcgagcac ctggccgccg cctcgcggct caagaatgca
      901 ctacagttga acgcgtgaag gacaatggtc tcttccggct cgtgggtttt gctccaacag
      961 ttgcggtgtg cattctcgcg gcgtgtcaag acgtccgagt cgatcctttt gctcgcgaag
     1021 cacgtccgga taccggagcg acaaccagtc ttgacgcggg aaggctagcc tgtccgccgg
     1081 aaatgatcgg atttgcgtcg gtcagtggtt cgacccagcc gaccacaggc ggtggcactg
     1141 cgcgtccggt cacggtttcg accctttctg acctggttag cgaggcggga tcggtgtcgc
     1201 cagccgtgat cgtgatcaag gggatggtgg tggttccacc ggccagccag cccctccaga
     1261 ttgaggtcgg ctccaacaag accatcgtcg gcgcggacgc gagctcagga ttgacgggcg
     1321 ggggcttcat catcgatgcc acccgcaacg tcatcattcg aaacctcgtg atctcattcc
     1381 cagtaggaac ggacggcatc gccgtccagg ccgcccagca tgtctggata gaccactgtg
     1441 aactctacag cgacacgaac cccggccgcg cgacggacta ctacggatgg ctcgtcaaca
     1501 tcaagcatgc ttccgatttc gtgaccgtgt cttggacgcg gtttcacgac cactttaaca
     1561 cggcgcaaat cgggcattcg gacgacaatt ccgccgagga tttagggcat ctgacggtca
     1621 cctaccatca caatctattc gagcgaacgc actccggttc tccccgagtt cggtttggga
     1681 ccgtgcacgt tttcaataac gactacgacg ccgtcagcga ctacgccgtc gcatcgcagg
     1741 atcaagctca ggtaagagtc gagcagaact attttcaaac cgttgccgag cctctcacta
     1801 atacccacga aatgactccc cctggggcta tcggggacat cctaaatacc tatagcgtag
     1861 attccggtcc caacgttttg gctggcagcg gcgcccctgc cttcaccttg gatgcccttc
     1921 cgtatccgtt tatgacggat tcgaccagct cggtccgtgc gatcgtctcg agttgcgcgg
     1981 ggcctggtcg gatttgacca ggctcaaaac cagtgtgcct cgatcgcggt tagcacggtc
     2041 acgaaaccgg gtccccacgg cagcgccgcg ggtccgccgg ccgacgcgag aggtcggcgg
     2101 aatgcctcgc cacctatcag ttcgccccga agcgcgatcg ttcgggagag tgcaactgtg
     2161 ccgccgatcg taccgcaccc cgcgacaccg acgccggtct gagccgtgcc ggcccaaccg
     2221 gctgtcggat tgcccgacac atgcagcgcg atcataagcc cgcccacccc g
//
LOCUS       DASWVF010000357         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_34944, whole genome shotgun sequence.
ACCESSION   DASWVF010000357 DASWVF010000000
VERSION     DASWVF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34944"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VGD55_06380"
     CDS             <1..834
                     /locus_tag="VGD55_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEY0870004.1"
                     /translation="TGQLLGTANAGAAHTTVHEGAVYVHQGETYVVREFCVDDAVALV
                     SPAAVDYFTTARDVTDIRITATSRTTLWGSATMSFGSVDVTTQVVSYLKRRVMSGEVL
                     GEQPLDLPQRQLSTRAVWWTVGDDQLADAGLAWADLPGAAHAAEHASIGLLPLVATCD
                     RWDIGGVSTARHEDTGRLTVVVYDGYPGGAGFAERGYEAARTWLSATRDAIAACGCET
                     GCPSCVQSPKCGNGNEPLDKAGAIRLLDSLLSGAGVCPTPALPLPLPPGVMSDQPALG
                     H"
     gene            complement(803..1213)
                     /locus_tag="VGD55_06385"
     CDS             complement(803..1213)
                     /locus_tag="VGD55_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEY0870005.1"
                     /translation="MTRQRLGGVMVAESDEGSASLWVLALAMVLWISVAGVVLVGVAI
                     VARHRAATAADLSALAAASAVQRAQLRDDPNASAQACTAARDISAANNATVESCEVSG
                     AVVDVATTVAIPALAALRFLGLDSVSAQARAGPT"
     gene            complement(1210..1626)
                     /locus_tag="VGD55_06390"
     CDS             complement(1210..1626)
                     /locus_tag="VGD55_06390"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEY0870006.1"
                     /translation="MFLSKWFIARRCRGQTWADGGDVTADGYVTAETAMVLPVLALLL
                     AVGLWAVAVAGAQVRCVDAARDAARAAARGESDAIATAVGRAAAPGDAQIELSHRGGV
                     VVVTVTARVGGGLGPLAAIPAPMVTATATAQSEDGP"
     gene            complement(1613..1852)
                     /locus_tag="VGD55_06395"
     CDS             complement(1613..1852)
                     /locus_tag="VGD55_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEY0870007.1"
                     /translation="MSRARAIARWLIRAAARRISRTIPDRLRTARDAGMSTAEYAIGT
                     VAACAFAAVLYKVVTSTQILDLLSGVVSRALHVPF"
     gene            complement(2000..2653)
                     /locus_tag="VGD55_06400"
     CDS             complement(2000..2653)
                     /locus_tag="VGD55_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0870008.1"
                     /translation="MSGSQALPGRAPDASPFVVVAATFGLVVGVAVALSAGAVVGCAF
                     GSATAFGVLRLTSFARARGPTVSRGPIESVGRQIPAALDLLAACLSAGAALAQALAAV
                     GAAFDGEVGTVLSAVARLAMLGAPIETAWSSCLNDRRWAPIARAVIRAHYSGAALTDV
                     LVHLADDRRRGLRADAQIAAQRAGVKAVMPLGVCFLPAFVLVGVVPVVAGFAHALWR"
     gene            complement(2732..>3095)
                     /locus_tag="VGD55_06405"
     CDS             complement(2732..>3095)
                     /locus_tag="VGD55_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0870009.1"
                     /translation="LVGAEPGSSGLRRLAACWRVAAASGASLAPAIDRVADALQDEID
                     VGRDVTSTLAGPRATVQLLAGLPVLGLLLGTAIGARPVEFLLGSGQGLACLIAAAAFD
                     SCGVVWARRIASRAARLR"
BASE COUNT          550 a         1080 c         1038 g          427 t
ORIGIN      
        1 accggccaac tgctcggcac ggcgaatgcc ggagcggcgc acacgacggt gcacgagggt
       61 gcggtctacg tgcaccaggg cgagacatat gtggtgcgcg agttctgcgt tgacgacgcc
      121 gtcgcgctgg tgagccccgc agctgtcgac tacttcacga cggcccgcga cgtgaccgac
      181 atcaggatca ccgcgacctc tcgaaccacc ctctggggtt cggcaacgat gagcttcggt
      241 tcggtcgacg tcaccacgca ggtggtctcg tacctgaaga ggcgggtcat gagcggggag
      301 gtgctcgggg agcaaccgct cgacctaccg caacgacaac tctcgacgcg ggccgtgtgg
      361 tggaccgtcg gcgacgatca actcgccgat gccggcttgg cgtgggctga tctgcccggc
      421 gccgcgcatg ccgccgagca cgcctcgatc ggcctgctgc cgctggttgc tacctgcgac
      481 cgatgggaca tcggcggcgt ctcgaccgct cgccatgaag acaccggccg actcaccgtc
      541 gtcgtgtacg acggctatcc gggtggcgca ggattcgccg aacgcggcta cgaggcggca
      601 cgcacctggc tcagtgcaac gcgcgacgcg atcgcggcgt gcgggtgcga gacgggctgc
      661 ccgtcgtgcg tgcagtcgcc caaatgtggc aacggcaacg agcctctcga caaggcgggc
      721 gcgatccgac tgctcgatag cctcctatcg ggcgccggcg tgtgtcccac accggcgctg
      781 ccgctgccgc tgccgccggg ggtcatgtcg gaccagcccg cgcttgggca ctgaccgagt
      841 cgagaccgag gaagcgcagc gccgcaagcg ccgggatcgc aacggtcgtc gcgacgtcga
      901 cgaccgcacc gctcacctca cagctttcga cggttgcgtt gttggcagcc gagatgtctc
      961 gcgctgctgt gcacgcctgc gctgatgcgt tcggatcgtc acggagttga gcacgttgca
     1021 ccgccgacgc ggcagctaac gccgacaggt cggcagctgt ggctgcacga tgacgcgcga
     1081 caatcgcgac gccgacgagc actacgcccg cgaccgagat ccacagcacc atcgctaggg
     1141 cgagcaccca caggctcgcc gacccttcgt ccgattcggc caccatcacg cctcccagac
     1201 gctgccgggt catgggccgt cttctgactg ggccgtcgcg gtagccgtca ccatcggagc
     1261 cgggatcgcc gcaagtggac cgagccctcc gcccacgcgg gcggtcacgg tcacgaccac
     1321 tacgccacct cggtggctca actcgatctg cgcgtcgcca ggtgcagcag ctctgccgac
     1381 ggccgttgcg atcgcatcgg actcacctcg tgccgccgcg cgcgctgcgt cacgcgctgc
     1441 gtcgacgcaa cgcacctggg cacccgcaac tgcgacagcc cacaggccga cggccagcag
     1501 gagcgcgagc actggaagca ccatcgccgt ttcggcggtg acgtacccgt cggcggtgac
     1561 gtcaccaccg tcggcccagg tttgcccgcg gcaacgacga gcgatgaacc acttagaaag
     1621 gaacatgcag tgctcgcgac acgaccccgc tcagcaaatc gaggatttgg gtgctggtca
     1681 ccaccttgta gagcacggca gcaaacgcac acgcggcgac agtgccgatc gcgtactcgg
     1741 ccgtcgacat ccccgcgtcg cgcgcggtgc gcaacctgtc ggggatcgtc ctactgatcc
     1801 tgcgcgctgc ggctcggatc aaccatcgtg caatagctcg ggctctcgac atgcgggctc
     1861 ctctttcgac gtgcgttcgt ttcgacgtgc gctggctaaa tcgggcgcaa gacgagcatg
     1921 gagtgcgagc gctcaccgag atggtccgcg acgccgaact gtggatcgcg gcgcgcagtc
     1981 atcgccttgt ggacgaacgt catcgccaca gcgcgtgcgc gaaccccgcg accacgggaa
     2041 cgacaccgac gagaacgaat gccggaagga aacacacccc caggggcatt actgctttca
     2101 cacccgctcg ttgcgccgcg atctgcgcat cggcacgcaa cccgcggcga cggtcgtcag
     2161 cgagatgaac gaggacgtcg gtcagtgcgg cacccgagta gtgcgcgcga attactgcgc
     2221 gggcgatggg cgcccaccgc cggtcgttca ggcaactcga ccacgccgtc tcgatcggtg
     2281 cccccaacat cgccagccgt gcgaccgcag acagcacggt gccgacctcg ccgtcgaatg
     2341 cagcaccgac tgcggcgagt gcctgcgcga gtgccgctcc agccgacaag cacgcggcaa
     2401 gcagatcgag cgcggcagga atctggcgac cgacagactc gataggtccg cgggagacgg
     2461 tgggtccgcg ggcccgagcg aaggacgtga ggcgcagcac gccaaatgct gtcgccgacc
     2521 cgaacgcgca tccgacgacg gcgccggccg acagtgcgac tgcgacgccc acaaccaacc
     2581 cgaaggtggc cgcgacgacc acgaatggtg aagcgtcggg cgcacgtcca ggtagggcct
     2641 gcgatccgct cacttcaaga acattggcaa ggcggcggcc ggcggcgcca acaggtcgtc
     2701 gcgacggggc gcgggccgtc tgagccacgc gctatcgcag cctggctgcg cggctcgcga
     2761 tgcgtcgtgc ccatacgaca ccgcaactgt caaaggccgc ggccgcgatc aggcaggcaa
     2821 ggccctgccc ggatccgagg aggaactcga ccggacgcgc accaatcgcc gtcccgagaa
     2881 ggagaccgag caccggcaga cccgcaagca gctgaacggt ggcgcgcggg cctgcgaggg
     2941 tcgatgtgac gtcgcgaccg acgtcgattt cgtcctgcag tgcgtcggcg acccgatcga
     3001 tggcaggtgc aagggaggca cccgatgctg ccgcgactcg ccagcaggca gcgagtcggc
     3061 gcaacccgct ggatccgggc tcggcgccga cgagg
//
LOCUS       DASWVF010000358         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_340740, whole genome shotgun sequence.
ACCESSION   DASWVF010000358 DASWVF010000000
VERSION     DASWVF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340740"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGD55_06410"
     CDS             <1..234
                     /locus_tag="VGD55_06410"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY0870010.1"
                     /translation="ADVDAALALIIEQAIDTLAGSDARYLRECEEASCGALFLDYTGR
                     RRWCSTPACGIRARVRAHRARQRYEVPAGPAGR"
     gene            complement(201..503)
                     /locus_tag="VGD55_06415"
     CDS             complement(201..503)
                     /locus_tag="VGD55_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870011.1"
                     /translation="MSAALISTIALIYAGFSVLGVAAGVSILRKAGYSPWWVLTGFVP
                     LVNVVMVVAFAFADWPVLQEHRRDHRLRTAQIAGDPRFTYSPAAPTYLPAGPAGTS"
     gene            703..1230
                     /locus_tag="VGD55_06420"
     CDS             703..1230
                     /locus_tag="VGD55_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870012.1"
                     /translation="MAEIDGNTDEAFIRALYEEHSRPLLGYALRLTNGDRHRAEDVVQ
                     ETLLRAWRHPEAIHRSPDAVRPWLFTVARHIAVDAYRARKARPPEAGPDGLEIVAVDD
                     DVDRALEAWQVTDALASLTPDHRRVLIETYYRGRSVAEAATELGVPAGTVKSRTYYAL
                     RALRLVLEERGVTEA"
     gene            1227..1943
                     /locus_tag="VGD55_06425"
     CDS             1227..1943
                     /locus_tag="VGD55_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0870013.1"
                     /translation="MSAGGCAEIRLSLGAYVLGALDPADRSRVDAHLATCAECRDELA
                     SLAGLPGLLGRVSRAEVEDEPSDPGPQLLDRLLNAAATQRRQDRRRRWLTSVAAAVIA
                     LAGIGVAIGVGQDRHQTVSATKPPAGVSETFTATNPQTHVQASVTEWKKGWGASLQVT
                     VTGVTAELAGARCQLIAVGPGGKTDVAASWAAPSEGYTGTSKVDANGATALASSDITA
                     FKVVGSDGATLVWVPAVWTT"
     gene            1993..2448
                     /locus_tag="VGD55_06430"
     CDS             1993..2448
                     /locus_tag="VGD55_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0870014.1"
                     /translation="MTLTSIVVLLVLVALTTAAGLIWRSRNGRVRALPDGAMLTASDL
                     GIALGERATLVHFSTAFCQPCRAARVTIGEVSAMVEGVAHVEIDAESHLDLVRRLDIM
                     RTPTVFILDAAGRIVQRASGAPRKADVLAALGRAMPATSTHRRSGAAAA"
     gene            2682..>3063
                     /locus_tag="VGD55_06435"
     CDS             2682..>3063
                     /locus_tag="VGD55_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEY0870015.1"
                     /translation="MADAAGNPALVDPRALRFAATLTAIVLAVVLLTSNGYVLAAQAV
                     VFAIGAIAGLRRAPYGVLFRTTIARRLGPPAEREPEAPPRFAQAVGLGFAAIGVIGFA
                     VGFGWLGVVATAFAFIAAFLNAAFG"
BASE COUNT          497 a         1008 c         1041 g          517 t
ORIGIN      
        1 gccgacgttg acgcggccct cgcgctgatc atcgagcagg cgatcgacac tctcgcaggt
       61 tccgacgccc ggtacctgcg cgaatgtgag gaggccagtt gcggtgcgct ctttctcgac
      121 tacaccggtc ggcggcgttg gtgctcgacc cctgcgtgcg gaattcgagc ccgcgtccgc
      181 gcgcatcggg ccagacagcg ctacgaggta ccggccggtc ctgccggaag gtaggtcgga
      241 gccgccggcg agtaggtgaa tcgggggtcg ccggcgatct gtgccgtgcg caaccggtgg
      301 tctcgccggt gctcctggag caccggccaa tccgcgaagg cgaacgccac caccatgacg
      361 acgttcacga gcgggacgaa cccggtcagt acccaccagg gcgagtagcc cgccttgcgg
      421 agaatcgaca cgccagcggc caccccgagc acgctgaagc ccgcatagat caaggcgatc
      481 gtgctgatca gtgcagcgct catggcagcc tcctcggcgt caagatcggc gcgatggcac
      541 cgttcctgaa acgccaagga ccctcagaaa cctggcacga tcactagtcg tggagtgtcc
      601 ggaccagtcg aggaacccca acgctcgccg gccaggcgcg gcgtgagtcg ctgggcggcg
      661 gacccaggtg aaccgcgacg gatcgggcga cgttcacggg atgtggctga aatcgatggg
      721 aacacggacg aggccttcat ccgtgccctc tacgaagagc actcccgtcc cttgctcggt
      781 tatgcgctgc gccttaccaa tggcgatcgg catcgagcgg aggacgtcgt ccaagaaacg
      841 ttgttgcgcg cctggcgcca cccggaagcg attcaccgta gtccggacgc cgttcggccg
      901 tggctgttta ccgtggctcg ccacatcgcc gtcgacgcct accgggctcg aaaggcaaga
      961 ccacccgagg ccggtccaga cggcctcgaa atagtcgcgg tcgacgacga cgtggatcgt
     1021 gctttggagg cgtggcaagt gaccgatgcg ctcgcgagcc tgacgccgga ccatcggcga
     1081 gtgctgatcg agacgtacta ccgcggccga tccgtcgccg aggctgccac cgaactaggg
     1141 gtgccagctg gcactgtcaa atcgcgcacg tactacgcgt tacgcgccct acgcttggtt
     1201 ttagaagaaa gaggagtgac cgaagcatga gcgccggtgg atgcgcggag atccgactgt
     1261 ccctgggcgc ttacgtgctc ggggcgctcg accccgccga tcgcagtcgt gtcgacgccc
     1321 atctggcgac ctgcgcagag tgccgcgacg agttagcgtc actcgccggg ctgcccggcc
     1381 tgctcggtcg agtgagccgg gctgaggtcg aggacgagcc gagcgatcca ggtccgcaac
     1441 tgctcgatcg cctcctcaat gccgcagcaa cgcagcgccg gcaagaccgt cgccgccgct
     1501 ggctcacctc ggtcgcggcc gcggtgatcg cgctcgcggg gatcggtgtc gccatcgggg
     1561 tcggccagga caggcaccag acggtgtcgg cgacgaaacc cccggctggc gtttccgaga
     1621 ccttcacggc caccaatccg cagacccacg tgcaggcaag cgtgaccgag tggaagaagg
     1681 gctggggcgc ctcgctccag gtcaccgtca ccggagtcac ggccgagttg gccggcgcgc
     1741 gttgccagct gatcgcggtt ggccctggcg ggaagaccga cgtcgcggcg agctgggctg
     1801 cgccgagcga gggctacacc ggcacctcga aggttgacgc gaacggggca accgcgttgg
     1861 cctcgagcga tatcactgcc ttcaaggttg tgggatcaga cggcgcgacc ctggtctggg
     1921 tgccggccgt ctggacgacc tagccagaac tggcggggac taacaccaga tcagctgcgg
     1981 ttgaatggtc tcgtgaccct gacgtcgatc gtcgtgttgc tcgtgctcgt ggcgctgacc
     2041 acggccgcgg gtttgatctg gcggtcacga aacgggcgag tgcgcgcgct tccggatggc
     2101 gccatgctca cggcgtccga cctgggtatt gctcttggag aacgcgcaac cctggtgcac
     2161 ttctcgacgg cgttctgcca gccgtgccgg gccgcccgcg tcaccattgg cgaggtcagc
     2221 gcgatggtcg aaggcgtagc gcacgtcgag atcgacgcgg agtcgcacct cgacctcgtg
     2281 cggcgcctcg acatcatgcg gaccccgacc gtgttcatcc tcgacgcggc cggtcgcatc
     2341 gttcagcggg cgagtggggc tccgcgcaag gccgacgtgc tagcagcgct cgggcgggcg
     2401 atgcctgcga cgagtactca tcggcgttct ggggcggcgg cggcttagaa ggtgacaggg
     2461 actggacggc gtccgtacca tcgagtcatg tatcaggtga aggtagacga gtcgaccagc
     2521 atgctggtgc tcgtcaagag gcgcgcggtc gacctgtgcc gcgtggccgc ctgcctgtgt
     2581 caaaactcct gattgggtcg gcgcagccct aacacgcatt gacgccggac ctgccgcgat
     2641 gtgattcctt cccttccatc gtttcgctca ggagctactc catggcagat gcagcgggca
     2701 accccgccct cgtcgacccg cgcgcgttgc ggtttgccgc gaccctcact gcaatcgttc
     2761 tcgctgttgt tctgctgacg agtaacgggt acgtgctcgc tgctcaggct gtcgtgttcg
     2821 cgatcggtgc gatcgccggg ttgcgccgcg cgccgtatgg ggtcctgttc cgcaccacga
     2881 ttgcgcgccg cctcgggcca ccagccgaac gcgagcccga ggcaccgccg cgattcgccc
     2941 aagcagtcgg gctcggcttc gcagcgatcg gcgtcatcgg cttcgcggtc ggatttgggt
     3001 ggctcggagt cgtcgcaact gcgttcgcct ttatcgccgc atttctcaac gcagccttcg
     3061 gct
//
LOCUS       DASWVF010000359         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_442923, whole genome shotgun sequence.
ACCESSION   DASWVF010000359 DASWVF010000000
VERSION     DASWVF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442923"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /gene="pepN"
                     /locus_tag="VGD55_06440"
     CDS             complement(<1..2103)
                     /gene="pepN"
                     /locus_tag="VGD55_06440"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0870016.1"
                     /translation="MPGNNLSRDEAADRARLIGPASGASHPGSPDQPVPSLQTKVLLD
                     LTAGAAKPGHFVSTSTIRFSCNEPGAATFVDLVADEIRSITLNGQSIEPATNRDADHI
                     KLKNLQAENELEIVAECRYMHTGEGLHRFTDPVDGRVYLYTQFETADAHRVYACFDQP
                     DLKTTFELDVIAPADWQVVSVTAPDLAPQQLEESPDTAASAHWHFPATPPISTYITSV
                     IAGSYHVVRDVHRGLAAEIPLGIFCRKSLAPYLDADDIFLVTKQGFGFFEELFQRPYP
                     FGKYDQLFVPEFNAGAMENAGSVTFLEDYVFRSRVTDTAYESRAGTILHEMAHMWFGD
                     LVTMRWWDDLWLNESFATYMANLATVEATRWPDAWTTFANEEKTWACRQDQLSTTHPI
                     AADIVDIEAVETNFDGITYAKGAAVLKQLVAWVGHEPFFNGLRDYFDLHAWGNSTLHD
                     LLRSLERASGRDLSAWSKEWLESAGINTLRPEVETDAEGLIISFAVLQEAPAEHPTLR
                     SHRLAVGCYERTPSGLSRTRRIELDVVGARTEVSELVGTRQPDLVLVNDDDLAYAKIR
                     LDERSLLTVTEAIGELTASLPRALCWAAAWDMTRDAEMAARDYLRLVIHLLDTETDIG
                     VLQSVLRQARLAVQRWSDPGFRDTGLDALAAACHAHLRHAEPGSDYQLALVRALSACA
                     NSPVYVDIIASVLAGETVF"
     gene            2337..2819
                     /locus_tag="VGD55_06445"
     CDS             2337..2819
                     /locus_tag="VGD55_06445"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEY0870017.1"
                     /translation="MRVYLGSDHAGYELKAHLIGWLTDAGHEPVDCGPPTYVADDDYP
                     PYIIAAALRVVADRGSLGIVIGGSGNGEAIAANKVKGIRAALAWNEDTATLARQHNDA
                     NVVSIGARQHTIGEATRLVEVFLATDFSGEARHVRRLALVTGYEETDVPPPLPEPDAS
                     "
BASE COUNT          509 a         1005 c         1048 g          503 t
ORIGIN      
        1 gaagacggtt tcgccggcca acaccgacgc gatgatgtcg acgtacaccg gtgagttcgc
       61 gcacgcagac agcgcccgaa ccaacgcgag ctggtagtcg ctgcccggct cggcgtggcg
      121 cagatgcgcg tggcaggcgg cggccagcgc gtcgagcccg gtgtcgcgaa acccggggtc
      181 agaccaacgc tgcactgcta gccgcgcctg ccgcagcacg ctttgcagca cgccgatgtc
      241 ggtctcggtg tcgagcaggt ggatcaccag ccgcaagtag tcgcgcgccg ccatctcggc
      301 gtcgcgcgtc atgtcccacg cagcggccca gcacaatgcg cgcggcagcg acgcggtgag
      361 ctcgccgatc gcctcggtca ccgtcagcag cgaccgttcg tcgagccgga tctttgcgta
      421 cgcgaggtcg tcgtcgttga cgagcacgag atcgggctgc cgggtgccga ccagctccga
      481 cacctcggtg cgggcaccga cgacgtcgag ctcgatgcga cgggtgcgcg acaggcccga
      541 cggggtgcgc tcgtagcagc ccacggcgag ccgatgcgag cggagtgtcg gatgttcggc
      601 cggtgcctct tgcagcacgg cgaaggagat gatcagcccc tcggcgtccg tctcgacttc
      661 tggacgcaag gtgttgatgc ccgcgctttc gagccattcc ttcgaccagg ccgagaggtc
      721 gcggcccgac gcccgctcga gcgatcgcaa caggtcgtgc agggtgctgt ttccccaggc
      781 gtgcagatcg aagtagtcac gcaggccgtt gaagaacggt tcatggccga cccacgcgac
      841 gagctgcttg agaacagccg cgcccttcgc gtaggtgatg ccgtcgaagt tcgtctcgac
      901 ggcctcgatg tcgacgatgt cggccgcgat cgggtgcgtg gtcgacagct gatcctgccg
      961 acacgcccag gtcttttcct cgttggcgaa ggtggtccag gcgtcgggcc agcgggtcgc
     1021 ctcgaccgtc gcgaggttgg ccatgtaggt ggcgaacgac tcgttcaacc acaagtcgtc
     1081 ccaccagcgc atcgtgacga ggtcgccgaa ccacatgtgc gccatctcgt gcaggatcgt
     1141 gccggcgcgg ctttcgtacg cggtgtcggt gaccctcgac ctgaagacgt agtcctcgag
     1201 aaacgtgacc gagccggcgt tttccatcgc gcccgcattg aattccggca cgaacagctg
     1261 gtcgtacttc ccgaaagggt atggccgctg gaacagctct tcgaagaacc cgaaaccctg
     1321 cttggtcact aagaagatgt cgtcggcatc gaggtagggc gccagcgatt tccgacagaa
     1381 gatgcccagc gggatctcag ccgccagccc tcggtgcacg tcacgcacga cgtgatacga
     1441 gcccgcgatc accgacgtga tgtaggtcga gatcggtggg gtcgccggaa aatgccaatg
     1501 ggcggacgcc gcggtgtcgg gcgattcctc aagctgctgc ggtgccaggt cgggggcagt
     1561 caccgacacg acctgccaat cggcaggagc gatgacgtcg agctcgaagg tcgtcttcag
     1621 gtcgggctgg tcgaagcatg cgtacacgcg gtgcgcgtcg gcggtctcga actgggtgta
     1681 gagatagacc cgaccatcga cgggatcggt gaagcggtgc aggccctcgc cggtgtgcat
     1741 gtaccggcac tcggcaacga tctcgagctc gttctcggcc tggagattct tcagcttgat
     1801 gtggtcggca tcgcggttcg tcgccggctc gatgctctgg ccgttcaggg tgatcgagcg
     1861 gatctcgtcg gcgaccagat cgacgaacgt cgctgcgcct ggctcgttgc agctgaatcg
     1921 gatcgttgac gtcgaaacaa aatgacccgg cttggcggct ccggcggtca ggtcgagcag
     1981 gaccttggtc tggagcgacg ggacgggctg gtctggcgag cccgggtggc tggcgccgga
     2041 ggccggccct ataaggcgtg cccggtcggc cgcctcgtca cggctcaggt tgttgcctgg
     2101 caccgatgcg agcctctctg tcggatcgtg tcgctgtcgg atcgtgtcgc ggtcggatcg
     2161 tgtcgctgtc ggatcgtgtc gcgcagttaa acggcggtgc agcgatcttc gcacggcgac
     2221 gcagcgcggc gcgaaatgca ccccactgtg gcctaccccg gtgcgaaacc ggccacgcgc
     2281 cgaccgggct gcactctcct taccgtgcgc gcacgggtcg tggcagactc cgcagcatgc
     2341 gcgtctacct agggtccgac cacgccggat acgagctgaa ggcccacctg atcggctggc
     2401 tcaccgacgc cggccacgaa ccggtcgatt gcgggccgcc gacctacgtc gccgacgacg
     2461 actacccgcc ctacatcatc gccgcagcgc ttcgggtcgt cgccgaccgc ggcagccttg
     2521 ggatcgtgat tggcggttca ggcaacggcg aggcgatcgc ggccaacaag gtgaagggca
     2581 tcagggccgc cctggcgtgg aacgaagaca ccgcgacgct cgcccgccag cacaacgacg
     2641 cgaacgtcgt cagcatcggc gcccggcagc acacgatcgg cgaggcgacc cgtctcgtcg
     2701 aggtcttcct cgcgacggac ttctcgggcg aagcgcgcca cgttcgccgc ctcgcgttgg
     2761 tgaccggcta cgaagaaacc gacgtcccac cgccgctccc ggagcccgac gcttcctgac
     2821 gcgacgcggg tcgcgctgtc ggttcgggct tttcaagagc atggcggacg tcgtcgggcg
     2881 tgggtcgtga gacatcgcgg gcacgcaacg cgccgacgac accaacttgc gaaggcccgg
     2941 cgccgaccaa cccgcgcagc gcgccggcgc cggcatgtgg aggcgtgttg gtagtccggt
     3001 tcgtagcagg ctcggaccgt cggggcacgc ctccagcgaa ccacatccga cagacacccg
     3061 gttac
//
LOCUS       DASWVF010000360         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_417408, whole genome shotgun sequence.
ACCESSION   DASWVF010000360 DASWVF010000000
VERSION     DASWVF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417408"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..996)
                     /locus_tag="VGD55_06450"
     CDS             complement(418..996)
                     /locus_tag="VGD55_06450"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870018.1"
                     /translation="MRADAKKNHSHLLSVARDVVTKDGAEASMRDIARRADVGLATLL
                     RHFPTREALFDELLRTKADALTQKAAELEASKPPEEALVSWFRDGVAFVQSYNGVCVM
                     MAGAHADPASALYASTTATHAAGARLLARAQAQGTARADMDGEDLFALMSALGWAAGQ
                     PSFAPRADHLFDIIAGAILTKPSRNDVKKTKR"
     gene            1098..1835
                     /locus_tag="VGD55_06455"
     CDS             1098..1835
                     /locus_tag="VGD55_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0870019.1"
                     /translation="MGKLAGKVAVITGGSSGMALETAKLFVEEGAYVFITGRRQEALD
                     QAVKAIGRNVTGVRADSANLADLDRLYEKVKSTKDHIDIVFASAGGGEVDKPIGSISE
                     RLVDDTLNLNIRGTLFTVQKALPLMREGGSIILNASNAHLKGYAGASVYAAAKAAVRS
                     FARSWTNDLKDRKIRVNSISPGAIGDTAAFAQISKELTDFFVSLVPLGRFGLSKEIAA
                     AVLYLASDDSRFTSAIDLVVDGGMSQV"
     gene            1848..2447
                     /locus_tag="VGD55_06460"
     CDS             1848..2447
                     /locus_tag="VGD55_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0870020.1"
                     /translation="MSYAIVGFGKIGQALAHAFARRNIDVTVASRHPPDALAPQARAI
                     GPAVVAKSLRDAVEADTIILATPFWEHREIAKTLPSWEGKTIIDATNALGAPEELDGL
                     LSSAFVARAFTGAKLVKGFNHLIAAILATDPTVEGGHRVVFLSSDDDSAIAPVAALAK
                     QLGFAPVNLGKLNEGGALVHARGGVWGALIFQDLFKKQQ"
     gene            complement(2454..>2799)
                     /locus_tag="VGD55_06465"
     CDS             complement(2454..>2799)
                     /locus_tag="VGD55_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870021.1"
                     /translation="DPRSMPGEQTLDGYSSADRVAFRSDSFIARLAAVRDGIGIGVLG
                     CFMGDREQSLVRLPFAVSDPTLNLALLVHVDLRQNVRVRAFSEHTYAALVAQRPLFQG
                     DAPQPTQRRRSG"
BASE COUNT          501 a          872 c          959 g          467 t
ORIGIN      
        1 tgaccggtgg cctgcaagcc tggctcgacg ccgggtacga cgctgatccg ccgtgagcgt
       61 atcaacggcg gtgctcgccc tgcgtgccgg attcgcagta gctcatgccg gaacggcagg
      121 gaaaggaccg tcgagccgca agcgatcagc cttttcggtg tggtacacgt tctgcgcctt
      181 ccgtgagagg gaggcaaaca tgagcctcaa gttgatgtta gtgacctgtg cggagacccg
      241 gagcacccgc ggggcccgcc gcctgacacg accgcggcat gtcagagcaa gtccgacggt
      301 gacgcctgca ccgtcaccca ccacgaccac caagaggacg gcgtgtgcgg caaggggaag
      361 tcaggggcgc tggcctgcgt gccgtaggtg atcgcgggcg cccagaccag attcaattca
      421 acgcttcgtc ttcttgacgt cgttgcgcga aggctttgtc aggatggcgc cggcgatgat
      481 gtcgaacagg tgatcggcgc gcggcgcaaa ggagggttgg ccggccgccc agccgagcgc
      541 cgacatgagg gcgaacaggt cctccccatc catgtcggcg cgcgccgttc cctgggcctg
      601 agcgcgggcg aggaggcgcg cgcccgctgc gtgcgtcgct gtggttgaag cgtaaagcgc
      661 agaggccggg tcggcgtggg cgcccgccat catgacgcag acgccgttgt agctttggac
      721 gaacgccacc ccgtcgcgaa accaggatac gagcgcctcc tccggcggct tcgacgcttc
      781 gagctcggct gccttctgcg tcagcgcgtc cgccttcgtg cgcagcagct cgtcgaacag
      841 ggcctctcgt gtggggaaat gacgaagcaa ggtggccaac ccgacgtcgg cgcgccgggc
      901 gatatctcgc atggacgcct cggcaccatc cttggtgacg acgtcacgcg cgactgaaag
      961 caggtggctg tggttcttct tcgcgtcggc tcgcatcggt tgccttgaca agcggatcag
     1021 tgaaccgcat attcggatca ctgatccgga tcatccggac cagtgatcca taagatacac
     1081 acgaaaggtg gccggtcatg ggaaagctcg cgggtaaggt tgcagtcatc acgggtggct
     1141 cgagcggcat ggcgctcgag acggcgaagc tgttcgtcga agagggcgcg tatgtgttca
     1201 tcactggccg gcggcaggaa gcgctcgacc aggcggtgaa ggcgattggc cgcaacgtga
     1261 ccggcgttcg cgccgactcc gccaacctgg cggacctcga ccggctgtac gagaaggtga
     1321 aatccacgaa ggaccacatc gacatcgtct tcgccagtgc cggcggtgga gaggtcgaca
     1381 agccgatcgg gtcgatctcg gagcgcttgg tcgacgacac cctcaacctc aacatccgcg
     1441 gtacgctgtt caccgtccaa aaggcgctgc ccctgatgcg cgagggcggc tcgatcatcc
     1501 tcaacgcctc gaacgcgcac ctcaagggat atgcgggcgc aagcgtgtac gcggctgcca
     1561 aggcggcggt caggtcgttc gcccgaagct ggacgaacga cctgaaggac cgaaagatcc
     1621 gggtgaacag catcagcccg ggcgcgatcg gtgacacggc ggcgttcgcc cagatctcca
     1681 aggagctgac ggacttcttc gtgtcgttgg tcccgttggg acgcttcggg ctttcgaagg
     1741 agatcgcggc cgcggtcctc tatctcgcct cggacgacag tcggttcaca tcggccattg
     1801 atctcgtcgt cgacggtggt atgtcgcagg tctgaaggag gagcatcatg agctacgcga
     1861 ttgtgggatt cggtaagatt ggtcaggccc tggcccacgc cttcgcccgt agaaacatcg
     1921 acgtgaccgt cgcaagccgt catccgcccg atgcgttggc gccgcaggct cgggcgatcg
     1981 ggcccgcggt ggtcgcgaag tctctgcgag acgcagtcga ggccgacacg atcatcctgg
     2041 cgaccccgtt ctgggagcac cgcgagattg cgaagactct gcccagctgg gaaggcaaga
     2101 cgatcatcga cgcgacgaac gcgcttggag cccctgaaga gctggacggt ctactgtcct
     2161 ccgccttcgt cgcgcgggcg ttcaccggcg ccaagctcgt caaaggcttc aatcacctga
     2221 ttgcggccat cctggctacc gatccgaccg tcgagggtgg ccaccgggtc gtgtttctgt
     2281 ccagcgacga cgacagcgcg atcgctcccg tggcggccct ggccaagcag ctcgggttcg
     2341 cccccgtcaa cttgggaaag ctcaacgagg gtggcgcgct ggtgcacgca cgcggaggcg
     2401 tctggggcgc gctcatcttc caagatttgt tcaagaagca gcagtagcgg aaactatccg
     2461 gagcggcggc gctgtgtcgg ctgcggcgcg tctccctgga agagggggcg ctgcgcgacg
     2521 agggcggcgt aggtgtgctc gctgaacgcg cgcacgcgta cgttctgcct gaggtcgacg
     2581 tgaacgagca gcgcgaggtt gagggtgggg tcggacaccg cgaacgggag ccgcacgagc
     2641 gattgttctc gatcgcccat gaagcagcca agcacgccga tgccgatgcc gtcgcgcacc
     2701 gccgcgagcc gcgcgatgaa cgagtcgctg cgaaaggcca cacggtcggc gctggagtag
     2761 ccgtccagcg tctgctcgcc cggcatcgag cgcggatcc
//
LOCUS       DASWVF010000361         7692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_315111, whole genome shotgun sequence.
ACCESSION   DASWVF010000361 DASWVF010000000
VERSION     DASWVF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315111"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGD55_06470"
     CDS             <1..290
                     /locus_tag="VGD55_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0870022.1"
                     /translation="LAAHEVEFGGQPLMLTVREFDLLAYLMGHSRQTFSREDLMRDVW
                     GWSFGDQSTVTVHVRRLRQKLEAASGGTWSPIATVWGVGYRYDPSTVSAVG"
     gene            290..1549
                     /locus_tag="VGD55_06475"
     CDS             290..1549
                     /locus_tag="VGD55_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0870023.1"
                     /translation="MADMTSMPLMAVMTLHAHDVALLVGVSAASSVVVTGVGLVLLKW
                     ARRRPLRDAALIVALTPVLTVVIASAAATMAMFLSRRQFGVLIATVVFAGIGGIVTSM
                     VLARTLMRGSDALRAAAKAMTRGEPYVGPVGTPSAELEALAAELALAHASLDEARRHE
                     RAIEDSRRELIAWVSHDLRTPLAGIRAMAEALEDDVVSTADDRSLYHRRITVEVDRLA
                     RMVDDLFELSRIHAGALQLSLREMELTDVVREAIATASPVAIAKGVRLRSAGLEADGP
                     VVDVDPYALTRVVGNLLGNAIRHTPHDGTIAVAADYDDDFVYVSVSDECGGIADDDID
                     RVFDVAFRGTQARTPAPDGGAGLGLAIARGIVDAHKGRIGAENVGPGCRFTIALPRAH
                     STLARSRTNARRDQPNGRIRGDAESLA"
     gene            1573..1764
                     /locus_tag="VGD55_06480"
     CDS             1573..1764
                     /locus_tag="VGD55_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870024.1"
                     /translation="MGGLVWWARIQWLQGQRPMWREHDATLAAVWRRDETGLHARRDP
                     HADPFIEPPDTAEPVAHST"
     gene            1805..3355
                     /locus_tag="VGD55_06485"
     CDS             1805..3355
                     /locus_tag="VGD55_06485"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0870025.1"
                     /translation="MLLADLVAVSDSVAATPARSEKIRLIGSALRTARTAGTDEVEIA
                     ATFLSGELRQRRTGVGWATLRDLPAPATVPQLTLTEVDEAFDRIAADAGTGTTARRAT
                     QLGMLFGAATDREQAFLRGLVTGELRQGALDGVMVDAIANAADVALAEVRRAFMVRGA
                     LAPIASVALRDGVEGLRALVLEVGRPVRPMLAQSAPDVATALAQTATAGPAAVEWKLD
                     GIRVQVHRNGSEVNVFTRTLDDVTDRVPELVEAALALDVRTVVLDGEAIALDQGGRPR
                     PFQETAARTSSRLDVEALRSSVPLAVFMFDVLHLDGRNLLDLPAADRHAVLAGIVPEK
                     LRVPRVVTGDEGEAARFFADAIDHGHEGVVVKSLQAPYEAGRRGAGWVKVKPRHTLDL
                     VVLAAEWGHGRRVGVLSNLHLGARDGSGGFVMLGKTFKGLTDELLAWQTVQFLELETH
                     RDSSTVYVRPELVVEIAFDGVQTSPRYPGGVALRFARVLRYRPDKLARDADDIGSVLA
                     IHSARSGS"
     gene            3424..4107
                     /locus_tag="VGD55_06490"
     CDS             3424..4107
                     /locus_tag="VGD55_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870026.1"
                     /translation="MRVLVVDDDPGVRESLRRSLIFNGYEVELAEDGQRALSSIALRR
                     PDAVVLDVMMPGLDGLETCRRLRAAGEDVAVLMLTAREAVADRVAGLDAGADDYLVKP
                     FALEELLARLRSLLRRSLVSGDVGQQLKFSDLVLDNGTREVWRGTRPISLTRTEFHLL
                     ELLMRHPRQVLERNQILEEVWGYDFPTTANSLEVYVGYLRRKLEAEGESRLIHTVRGV
                     GYSLRELPA"
     gene            4104..5549
                     /locus_tag="VGD55_06495"
     CDS             4104..5549
                     /locus_tag="VGD55_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0870027.1"
                     /translation="MSTEEIHSVGGMTLRARVSLLVAITAGFAVAAVSLAAYLTMRME
                     LFHELDSSLVSRAQRVAALQQQIPETLQTQPTLTLLASEVDLCLVSGAGDVECTAPGL
                     TKLMQAGPELAVARGTTAQSLRTLDINGTRYRVVAVPVGSDVALVLASSTKDTQTTLN
                     DLGLVLLFVGVVGIVIAAVAGLLVARAGLRPVERLTKAAERVGRTGDLTPIDVESADE
                     LGRLARSFNEMLAAVSSSRALQRQLVADAGHELRTPLTSLRTNLDLLAQSNAAGDRGL
                     PVADREALLLDVRAQVEELSGLVADLVELARDDRPSASAEELEFAAIVERAIERVRRR
                     APGVIFKVDLHSWLVVGETTALERAVTNLLDNAVKWSPADGVVEVSLDDGRRAVVDSG
                     PGIAEADLPHVFDRFYRSTDARAMPGSGLGLAIVRQAAERHGGSVFAGRAHEDGSGGA
                     RLVLDLPGRYPAPPVEHSGSLQQNWGSSVHE"
     gene            5542..7230
                     /locus_tag="VGD55_06500"
     CDS             5542..7230
                     /locus_tag="VGD55_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0870028.1"
                     /translation="MSDGYEGHDGPQQPWWASPSDQPLDPAVPDATAPAEPAEGAEPP
                     KPVLPRYGADPHLGSFPYNEAERPAYVAPPAPVAVPEYTVPDNVPPPIPTSPAYPPPP
                     PTYTAPAYPPPPTYTPPTHTPPTHMPPTYTPPAYAPPAYAPSPSPYGPPSYPPPTSSA
                     PAFPAATPYGEPAADPRATKAGRRAGWPSLFAVAGIAALIGALVAGGLVAATKKTTKT
                     AAPVATPSVSGSASTVAVQPAVSLPSGSVAQIAATLLPSVVSIIVTSGDSGDEGTGIV
                     LTADGSILTNNHVVESAASSGIIAVTLNDGRTVKATIVGRDPTTDLAVIKAAGVTGLH
                     PATLGQDSSLVVGQQVIAIGSPLGLSNTVTSGIVSSLNRPVCTQNCDGTGGGSASTPT
                     VLDAIQTDAAINPGNSGGPLVDMSGKVVGVNSAIATLDQGQTGSGAQSGSIGVGFSIP
                     IDEARRVVDELEKTGHANHALLGIGLQDSVDAVLHTPNGAKVVTVTSGGPAAKAGIQV
                     GDIIVKFDTRTIVDADSLIAATHAAVPDSTVTMSYYRGGHTTTTQLTLGSAASS"
     gene            complement(7234..>7692)
                     /locus_tag="VGD55_06505"
     CDS             complement(7234..>7692)
                     /locus_tag="VGD55_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-beta hydroxysteroid dehydrogenase"
                     /protein_id="HEY0870029.1"
                     /translation="VRSSIVRLAPTNHGDGDNGFMATLVSIARAKGVSGYVGDGSNRW
                     PAVHRLDSANLFRLAVEKAPAGSTLHAIADEGVPFRDIADVIGRHLNIPVVRVAPENA
                     GQHFSWLGGFVAIDSPASSALTRELLGWQPTQLGLIDDLDKGHYFDQPAA"
BASE COUNT         1225 a         2554 c         2558 g         1355 t
ORIGIN      
        1 acctcgcggc tcacgaggtc gaattcggtg gtcagccgtt gatgttgacc gtgcgcgaat
       61 tcgacctcct ggcctacctg atgggtcatt cgcggcagac cttcagccgc gaagacctca
      121 tgcgcgatgt ctggggctgg tcattcggcg atcagtcgac cgtgaccgtg cacgtgcggc
      181 ggcttcggca gaagctcgag gctgcgtcgg gtggtacctg gtcgccgatc gccacggtgt
      241 ggggagtcgg ataccgctac gacccgagca cggtatcggc ggtcggctga tggcagacat
      301 gacctcgatg ccacttatgg cggtgatgac gctgcacgca cacgacgtgg cgctgcttgt
      361 cggcgtctcg gctgcgagct cggtcgtcgt gacgggcgtt ggtctggtct tactgaagtg
      421 ggcgcgccgg cggccgttgc gtgacgcagc cctgatcgtc gcgctgacac cggttctcac
      481 cgtggtcatc gcgagcgccg ctgccacgat ggcgatgttc ttgtcgcgaa ggcaattcgg
      541 cgtccttatc gcgaccgttg tcttcgcagg catcggcggc attgtcacgt cgatggtgct
      601 cgctcgcacg ttgatgcgcg gcagcgatgc gctgcgcgcg gcagccaagg ccatgacgcg
      661 cggggagccc tatgtaggcc cagtcgggac gccgagtgct gagctcgagg cgttggcggc
      721 cgagcttgca ttagcgcatg cgtcgctcga cgaggcacgg cgacacgagc gggcaatcga
      781 agacagccgt cgcgagctca tcgcgtgggt cagtcacgat ctgcgcacac cactcgcggg
      841 gattcgcgca atggccgagg ctctcgaaga cgacgtcgtc tcgaccgccg acgatcgctc
      901 gctctaccac cgcaggatca cagtggaggt cgatcggttg gcgcggatgg tcgacgatct
      961 gttcgagctg agccgcatac atgcgggcgc attgcagttg tctttgcggg aaatggagtt
     1021 gaccgacgtc gttcgagagg ccattgctac cgcctcaccc gtcgccatcg cgaagggagt
     1081 caggttgcgg tcggcgggtc tcgaagctga tggcccggtc gtcgacgtcg acccttacgc
     1141 actcactcgt gtcgtcggca acctgctggg caacgcgatc cggcacaccc cacatgacgg
     1201 cacgatcgct gtcgcggccg actacgacga cgacttcgtc tacgtatcgg tcagcgacga
     1261 atgtggcggc attgccgacg acgacatcga ccgggtgttc gacgtggcgt ttcggggaac
     1321 ccaagcccga acccccgcgc ccgacggcgg tgctggcctt ggactcgcca tcgcgcgcgg
     1381 gatcgtcgac gcacacaagg gacgaatcgg tgcagagaat gtcggaccgg gctgccgctt
     1441 cacgatcgcc ttgccacgcg cgcattcaac gcttgcgcga tcacgtacga acgcgcgccg
     1501 agaccaaccg aacggtcgta tccgcgggga tgccgaatcg ctagcttgag acgtcaggct
     1561 agggggtggc ccgtgggggg ccttgtttgg tgggcgcgca ttcaatggct gcaaggccaa
     1621 cggccgatgt ggcgggagca cgatgccacg ttggcagcgg tctggcgtcg tgacgagacc
     1681 ggcttgcacg cccgccgtga cccgcacgct gacccattca tcgaaccgcc agacactgcg
     1741 gagcccgtcg cccactcgac ctgatccagc cgttcttgtc actgctgcac gcgagaatgg
     1801 cttcgtgctg ctcgccgatc tcgtcgccgt ctccgactcc gttgctgcca cgccggcgcg
     1861 atcggagaag atccggctca tcggtagcgc gcttcgcacc gcacgcaccg caggtaccga
     1921 cgaggtcgag attgccgcca ccttcttgtc gggcgagttg cgacagcgcc gcacgggtgt
     1981 gggttgggcg accttgcgcg atctgcccgc accggcgacg gtcccgcagc tgaccctgac
     2041 cgaggtcgac gaggcgttcg atcgcatcgc cgctgatgca ggcaccggca cgacggcgag
     2101 acgcgcaact caactgggca tgctcttcgg cgcggccacc gatcgcgagc aggcatttct
     2161 tcgtggtctg gtcacaggtg agttgcggca gggtgcgctc gacggcgtga tggtcgacgc
     2221 gattgccaac gcagccgacg ttgcgttggc cgaggtacgc cgtgcgttca tggtgcgggg
     2281 cgcgttggcg ccgatcgcct cggtcgctct tcgcgacggc gtcgaaggtc tgcgcgcact
     2341 cgttctcgag gtcggtcggc cggtgcggcc gatgctggct cagtcggctc ctgacgtcgc
     2401 gacagcgtta gcgcaaaccg cgacagcggg gcctgcagcc gtcgagtgga agctcgacgg
     2461 catccgggtg caggttcacc gcaacgggtc cgaggtcaac gtattcaccc gcaccctcga
     2521 cgacgtcact gaccgagtac ccgagctggt cgaggcggcg ctcgccctcg acgttcgcac
     2581 cgtcgtgctt gatggcgaag ccattgctct cgaccagggc ggtcggccgc ggccgtttca
     2641 agagaccgcg gctcgcacga gcagccgtct tgacgtcgag gcgcttcggt cctcggttcc
     2701 gctggcggtc ttcatgttcg acgtcttgca tctcgacggt cgcaacctgc tcgaccttcc
     2761 ggccgctgat cgtcatgccg tgctcgccgg catcgtgcct gagaaactgc gggtgccgag
     2821 agtcgtcacc ggcgatgagg gcgaggcggc gcgattcttc gccgatgcga tcgatcacgg
     2881 ccacgagggc gtcgtggtga agtcgttgca ggcgccttat gaagccggtc gtcgcggtgc
     2941 cggttgggtg aaggtgaagc ctcgacacac tctcgatctc gtcgtgctcg cagcggaatg
     3001 ggggcatggc cgacgcgtcg gcgtgctgtc gaacctccat cttggcgctc gtgacggcag
     3061 cggggggttc gtcatgctgg gcaagacgtt caaggggttg accgacgagt tactggcctg
     3121 gcagacagtg caattcctcg agctcgagac gcatcgcgat tcgtccaccg tctacgtacg
     3181 gcccgaactc gtggtcgaga tcgccttcga tggtgtgcag acgagcccgc gatacccagg
     3241 tggtgtcgca ttgaggttcg ctcgggtgct gcgctatcgc cccgacaagc tggcccgcga
     3301 cgccgacgac atcggctcgg tgctggcgat ccactcggcc aggtctggta gctagggcgg
     3361 ctccggcggt tcgacgaggc ccctttcagc attcattcag gcatcgcggg cacacttgca
     3421 ggcgtgcgcg ttctcgtcgt cgacgacgat cccggagtca gggagtcgct tcgccgttcc
     3481 ctgatcttca acggctacga ggttgagctc gccgaagacg gccagcgcgc gctgtcgtcg
     3541 atcgcgctgc gccgacccga cgcggtggtt ctcgacgtga tgatgcccgg tctcgacgga
     3601 ctcgagacct gccggcgact tcgtgcggcc ggcgaggacg tcgccgttct gatgctcacg
     3661 gcacgcgagg cggttgccga tcgggtggcc ggactcgacg ccggtgctga cgactacctc
     3721 gtcaagccgt tcgccctcga agaactgctc gcccgactgc ggtcgctgct gcggcgaagc
     3781 ctcgtcagcg gggacgtcgg tcagcaactg aagttctctg atctggtgct cgacaacggc
     3841 accagggagg tctggcgggg cacccggccg atctccctga cgcgtaccga gttccacctc
     3901 ctcgagttgc tcatgcggca cccgcggcag gtactcgagc gcaaccagat tctcgaagag
     3961 gtatggggtt acgacttccc gacgaccgcg aattcgctgg aggtctacgt cggttacctg
     4021 cgacgaaagc tcgaagctga aggcgagtcc cggctgatcc acaccgttcg aggcgtcggc
     4081 tattcgctgc gcgagctgcc cgcgtgagca ccgaagagat ccactcggtc ggcggcatga
     4141 cgttgcgcgc gcgagtgtca ctgctggtgg cgatcacggc gggatttgcc gtcgccgcgg
     4201 tgtcgctcgc cgcgtacctc accatgcgga tggagttgtt ccacgagctc gacagttcgc
     4261 tggtatcacg ggcgcagcgc gtcgccgctt tgcagcagca aattcccgag accctccaga
     4321 cccaaccaac gttgaccctg ctcgcctccg aagtcgacct ctgtctggtc agcggcgccg
     4381 gcgacgtcga atgcacggcg ccgggtctga ccaaactcat gcaggccggg cccgagcttg
     4441 ctgtcgcgcg cggtaccacc gcgcagtcgc ttcgaaccct cgacatcaac ggcacccggt
     4501 atcgcgtcgt cgcggttccg gttgggtcgg acgtcgccct ggtactcgcg agctcgacga
     4561 aggacaccca gacgacgctg aacgatctcg gtctggtgct gctgttcgtc ggggtggtcg
     4621 gcatcgtcat tgctgccgtc gccggtctgc tcgtcgcccg cgcaggcctt cggccggtcg
     4681 agcgtctgac gaaggccgcc gagcgggtcg gtcgcacggg ggacctcacc cccatcgacg
     4741 tcgagagcgc cgacgagctc ggcaggctcg cgcgcagttt caacgagatg ctcgcggccg
     4801 tgtcgtcgtc gcgagcgctg caacggcagt tggtcgctga cgcgggtcac gagttgcgca
     4861 ctccgctgac gagtttgcgc acgaatctcg acctgctggc tcaaagcaac gcagccggtg
     4921 accgggggct gccggtcgcc gatcgagaag cgctgctcct cgacgtccgg gctcaggttg
     4981 aggagttgtc ggggctcgtc gccgatctgg tcgaactcgc tcgcgacgac cgtccgagcg
     5041 ccagcgctga agagctcgag ttcgcagcga tcgtcgagcg ggcgatcgag cgggtgcgcc
     5101 ggcgtgcacc gggcgtgatc ttcaaggtcg acctgcacag ctggctcgtc gtcggggaga
     5161 cgaccgctct cgagcgcgct gtgacgaact tgctcgacaa cgcggtcaag tggagccctg
     5221 ccgacggcgt cgtcgaagtc agtctcgatg acggtcggcg tgctgtcgtc gactcgggcc
     5281 ctggcatcgc cgaggccgac ctgccgcatg tgttcgaccg cttctaccgg tcgaccgacg
     5341 cgcgagcgat gcctggatct gggctcgggc tggccatcgt gcggcaagcg gccgagcggc
     5401 atggcggtag cgtgttcgca ggacgcgctc atgaggatgg cagcggcggc gcgcgtctag
     5461 tcctcgatct gccgggccga taccctgccc caccggttga acattcgggt tctcttcagc
     5521 agaactgggg atcgtcagta catgagtgac ggatacgaag ggcacgacgg tccgcaacag
     5581 ccctggtggg catcgccgag cgatcaaccg ctcgacccgg cagtccctga cgcaacagcg
     5641 ccggcggagc ccgccgaggg cgccgaaccc ccgaaaccag ttctgccacg ctatggcgcc
     5701 gacccgcacc tcggatcttt tccctacaac gaagccgaac ggcctgcgta tgtagcaccg
     5761 cccgcgccgg tggccgtacc cgagtacacc gtcccagaca acgtgccgcc gccgatcccg
     5821 acgtcgccgg cttacccccc gccgccgccg acctataccg ctccggcgta cccaccgccg
     5881 ccgacctaca cgccaccgac acacacgcca ccgacacaca tgccaccgac ctatacgcct
     5941 ccggcgtacg cgcctccggc gtacgcgccg tcgccgtcgc cgtatgggcc gccgagctat
     6001 ccgccgccca cctcttcggc tccggcgttt cctgcagcca ctccgtacgg cgaacctgca
     6061 gccgatccgc gcgctacgaa ggccggacgc cgggctggtt ggccgtcgtt gttcgcggtc
     6121 gcgggtatcg ctgccttgat cggtgctctc gtcgcaggag gcctggtcgc ggccaccaag
     6181 aagacgacca agaccgctgc gcccgtagcg acgccatcgg tctcgggatc ggcgtcaaca
     6241 gttgccgtgc agccggccgt cagtttgccg tcgggctcgg tcgcgcagat tgccgcgacg
     6301 ctgttgccgt cggtcgtgtc gatcatcgtc acgtctggtg actcaggtga tgagggcacc
     6361 ggcatcgtcc tgactgccga cggcagcatc ctcacgaaca atcacgtcgt cgagtcggca
     6421 gcgtcgtccg gcatcattgc ggtcaccctg aacgatggcc gcacggtaaa agccaccatc
     6481 gtcggccgtg atccgaccac tgaccttgcc gttatcaaag ccgcgggcgt gaccgggctg
     6541 caccccgcca ccctcggcca ggattcgtcg ctagtcgtcg gccagcaggt gatcgcgatc
     6601 ggctcgccgc ttggcctgtc gaacaccgtc acctcgggca tcgtcagctc actcaaccgc
     6661 ccggtgtgca cccagaactg cgatggcacc ggcggcggct cggcatcgac gccgaccgtg
     6721 ctcgacgcga tccagaccga cgccgcgatc aacccaggca actccggcgg cccgcttgtc
     6781 gacatgtcag gcaaggtcgt cggggtcaac tcggcgatcg cgacgttgga tcagggtcag
     6841 acagggtcgg gcgcccagtc gggcagcatc ggcgtcgggt tctcgatccc gatcgacgag
     6901 gcgcgtcgcg tggttgacga gctggaaaag accggccacg ccaaccacgc acttctcggc
     6961 atcggacttc aggattccgt cgacgccgtg ctgcatacgc cgaacggcgc gaaggtcgtg
     7021 acggtcacca gcggaggccc cgcggccaaa gccggtatcc aggtgggcga catcatcgtg
     7081 aagttcgaca cgcgaacgat tgtcgacgct gattcgctca tcgctgccac ccacgcagcg
     7141 gtgccggact cgaccgtgac catgagctac tacagaggcg ggcacacgac aaccactcaa
     7201 ctcacgctcg ggtcagcagc gtcgagttag cactcaggcg gccggctgat cgaagtagtg
     7261 acccttgtcg agatcgtcga tgaggccaag ctgagtcggt tgccatccca acagttcgcg
     7321 cgtaagtgcg cttgaggccg ggctgtcgat ggcgacgaag ccgccaagcc agctgaagtg
     7381 ttggcccgcg ttctctggcg cgaccctgac caccggaatg ttcaggtgcc gcccgatgac
     7441 gtcggcgata tcgcggaacg gcacaccttc gtcggcgatt gcgtgcagcg ttgatccagc
     7501 cggtgccttc tccaccgcca agcgaaacag gttggcggaa tccagtcggt gaactgcggg
     7561 ccaccggttg gatccgtcac cgacgtagcc ggagacaccc ttggcgcggg cgatgctgac
     7621 cagcgtcgcc atgaagccgt tgtcgccgtc gccgtgattc gtcggagcca gtcgcacgat
     7681 cgacgaccgc ac
//
LOCUS       DASWVF010000362         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_213583, whole genome shotgun sequence.
ACCESSION   DASWVF010000362 DASWVF010000000
VERSION     DASWVF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213583"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VGD55_06510"
     CDS             complement(<1..420)
                     /locus_tag="VGD55_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870030.1"
                     /translation="MKVAMLGPIAWRTPPRHYGPWELVTSLLTEGLVARGIDVTLFAT
                     LDSHTTAKLDGVRKHGYADDPSVDGRVWEAMHVAHVLSRSHEFDLVHNQLDWLPLAFA
                     DHCAVPMVTTIHGFSGAGILPAYKRAHSHYVAISEADR"
     gene            529..3069
                     /locus_tag="VGD55_06515"
     CDS             529..3069
                     /locus_tag="VGD55_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY0870031.1"
                     /translation="MRTGPRPFRLAAALAVVVAAAAPAVVPAAAHAASAGPALTVDAA
                     AGRHAISTDIYGMNFADAGLATELGLTADRWGGNSTSRYNYTNNTHNTGSDYYFENIV
                     DSTSLDSFVAADLAHGTNPVVTVPAMGWVSKNSPSTHPFACAYKVSVYGAQQSTDPYD
                     PSCGNGKNTSGAQITGNAPSDTSVTENGALDQAMVTHLVQKFGSAAQGGVRSYEIDNE
                     PSLWSSTHIDVHPAPLSYDELWQKSLAAAQGIKAGDPTATVDGPGDWGWCAYFYAPDD
                     PGTCGPGPDRTAHGNLPLAPWYLQQFAAYDQAHGQRLLDYFDEHFYPQESGVSLSTAG
                     SAATQALRLRSTRTLWDPTYTDESWTADLGLGSVYLIPRMKQWVAQYYPGTKTAISEY
                     NWGGLESMNGALAQADVLGIFGSQGLDRAQLWSPPTSSEPGAFAFRMYRNYDGHGSRF
                     GDESVSASSGDQGQLSVYAAQRSTDGALTVQVINKTGGDLTSTLAVHNAPTSAAQVFT
                     YSGANLGAIVQGPGAAFSSGSLSYTFPANSITLLVIPTNIAVSRLGGANRDATAAAVA
                     LAAFPTAGSARAVVLARDDVYTDGLTGSPLASALGGPLLLTNPSLLSADAKTAIAHAL
                     PAGGTIYVLGGTSAITPQTTSALTALGYQVVRIGGADRYATASLVADQLVSAHSVDHV
                     YLATGLNFPDAESAADAAAVNHGVVLLTDGTSIPAATAAWQTAHPGMTVVAIGGAAAS
                     ADPSATPLVGADRYATAARVAASVLPTPAGVVIATGANFPDGLAGAAYAAHFGWAMLL
                     VSPGSTTLDADQASYLHGAASTAKNLVTVGQTVAVPPAAAGAVLAGLS"
     gene            3103..>3332
                     /locus_tag="VGD55_06520"
     CDS             3103..>3332
                     /locus_tag="VGD55_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870032.1"
                     /translation="MTELPVASAHLLQVVDVVDVGPRMRRITVAGAALAEFAAWPGQD
                     VVVHVSDGHGGSVSRRYTIRNLDNAMRLLDLDV"
BASE COUNT          524 a         1112 c         1137 g          559 t
ORIGIN      
        1 gcggtcggct tctgagatcg cgacgtagtg cgagtgcgcg cgcttgtagg cgggcaggat
       61 accggcgcca gagaatccat ggatcgtcgt gaccatcggc actgcgcagt gatcggcgaa
      121 cgcgagcggc aaccaatcga gctggttgtg cacgagatcg aactcgtgtg atcggctcag
      181 cacgtgcgcg acatgcatcg cctcccacac gcggccgtcg accgaggggt cgtcggcgta
      241 gccgtgcttt ctcacgccgt cgagcttcgc ggtcgtgtgc gagtcgagtg tggcgaacag
      301 cgtcacgtcg atgccgcggg ccaccaaccc ctcggtgagc aggctcgtca ccagctccca
      361 cgggccgtag tggcgcggcg gtgtgcgcca ggcgatcggc ccgagcatgg cgaccttcac
      421 cgggcctgtt cgctcctcat ggacgcattc tggcccaggc tgctccgata gggggaaatc
      481 aggcgcaagc aacgcaatcc gggacgtcgc gggcctaggc tgatcctcgt gcgcacaggt
      541 ccccgcccgt tccgccttgc ggccgcgctc gcggtggtcg tcgctgcggc tgcgccagcg
      601 gttgtgccgg ccgcggccca tgctgcttct gctggccctg ccctcaccgt cgatgccgct
      661 gctgggcgac atgccatcag taccgacatc tacgggatga acttcgccga cgcgggcctg
      721 gcgaccgagc tcggcctcac cgccgacagg tggggtggca acagcacgag ccggtacaac
      781 tacacgaaca acacccacaa caccggcagc gactattact tcgagaacat cgttgactcg
      841 acgtcgctcg actcattcgt cgcggccgat ctcgcgcatg gcacgaatcc ggtagtgacc
      901 gttcccgcaa tggggtgggt gtcgaagaac tcgccctcga cgcatccgtt cgcgtgtgcg
      961 tacaaggtct cggtgtacgg cgcgcagcag tcgacggatc cgtacgaccc gagctgcggc
     1021 aacgggaaga acaccagcgg tgctcagatc accggcaacg cgccgtccga tacgtcggtg
     1081 actgagaacg gggcgctcga ccaggcgatg gtgacgcacc tcgtgcagaa gttcggctct
     1141 gctgcgcagg gcggcgtgcg cagctacgag atcgacaacg agccgtcgtt gtggagcagc
     1201 acgcatatcg acgtgcatcc ggcacctctg agctacgacg agttgtggca gaagtcgctc
     1261 gcggcagcgc agggcatcaa ggcgggcgac ccgaccgcga cggtcgacgg ccctggtgac
     1321 tggggatggt gcgcgtactt ctacgcaccc gatgacccgg gcacgtgtgg gccaggtccc
     1381 gaccgcaccg cgcacgggaa cctgccgctg gcgccgtggt acctccagca gttcgctgct
     1441 tacgaccagg cgcacggcca gcgcctgctc gactacttcg acgagcactt ctatccgcag
     1501 gagagcggcg tttcgctgtc gacggccgga agtgcggcaa cgcaggcgtt gcggttgcgg
     1561 tcgacccgca cgctgtggga tccgacgtac accgacgaat cgtggaccgc ggatctcggc
     1621 ctcggctcgg tctatctgat cccgcggatg aagcagtggg tcgcgcagta ctaccccggc
     1681 acgaagaccg cgatcagtga gtacaactgg ggtggcctcg agtcgatgaa cggcgcgttg
     1741 gcgcaagccg atgtgctggg cattttcggg agccagggtc tcgaccgcgc gcagctatgg
     1801 agcccgccca cgtcgtccga gcctggcgcc tttgcgtttc gcatgtaccg caactacgac
     1861 ggtcacggtt cgcgtttcgg tgacgagagc gttagcgcgt cgagcggtga ccagggccag
     1921 ttgtcggtgt acgcggcgca gcgctcgacg gacggcgccc tgacggtgca ggtcatcaac
     1981 aagaccggcg gtgacctgac gagcacgctc gccgtgcaca acgcgccgac gtctgcagcg
     2041 caggttttca cttacagcgg cgcaaatctc ggcgcgatcg tgcaggggcc aggagccgca
     2101 ttctcgtcgg gcagtctttc gtacaccttc ccggcgaaca gcatcacgtt gctcgtgatc
     2161 ccgaccaaca tcgcggtgtc gcgccttggc ggcgccaacc gtgacgcgac cgcggccgcc
     2221 gttgcgctgg cggccttccc gactgctggg tcagccaggg ccgtcgtgct cgcgcgcgac
     2281 gacgtctaca ccgacgggct caccgggtcg ccgttggcga gcgcgctcgg tggcccgctg
     2341 cttttgacca acccgtctct gttatcggct gacgcgaaga ccgcgatcgc gcatgcgttg
     2401 cctgcgggcg gcacgatcta cgtgctcggg ggaacaagtg ccatcacacc gcagaccacg
     2461 tctgcgttga ctgcgctcgg ctatcaggtc gtgcggatcg gtggtgccga tcgatatgcg
     2521 acggcttctt tggtcgccga ccagctcgtg tctgcgcaca gcgtcgacca tgtctacctc
     2581 gcgaccgggc tgaacttccc cgatgcggag tcggccgccg acgcggcagc ggtcaaccac
     2641 ggcgtcgtgc tgctcactga cgggacgtcg atcccagccg cgacagcggc gtggcagacc
     2701 gcgcaccccg gcatgaccgt cgtcgcgatc ggcggcgcgg ctgcgagcgc cgacccctcg
     2761 gcgacgccgt tggtcggtgc cgatcgctac gccacggccg ctcgggtggc ggcaagcgtg
     2821 ttaccgacgc cggccggcgt cgtgattgcg accggcgcca acttccctga cgggctcgct
     2881 ggcgccgctt acgccgccca tttcggttgg gcgatgctgc tcgtgtcacc gggttcgacg
     2941 acgctcgacg ccgatcaggc gagctacctg cacggggcag cgagcacggc gaagaacctc
     3001 gtcacggtcg gccagacggt tgcggtaccg cccgccgcgg ctggggcggt gctggcggga
     3061 ctgagctgag cgtgaaaccg ggcgtgctgg atgatcgacc ctgtgactga gctgcctgtt
     3121 gcgagtgcgc acctgctcca agtggtcgac gtcgtcgatg tcggtcctcg gatgcgccgc
     3181 atcacggttg ccggcgccgc gctcgccgag tttgccgcgt ggccggggca ggacgtcgtc
     3241 gtgcacgtca gcgacgggca cggtggcagc gtcagccgtc gctacacgat ccgcaacctc
     3301 gacaacgcga tgcggctgct ggatctcgac gt
//
LOCUS       DASWVF010000363         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_391889, whole genome shotgun sequence.
ACCESSION   DASWVF010000363 DASWVF010000000
VERSION     DASWVF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391889"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGD55_06525"
     CDS             complement(<1..457)
                     /locus_tag="VGD55_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870033.1"
                     /translation="MLTPEHEAILTAALRAPSAHNAQPWRLEVRPDDQCYELHYDYTD
                     YLPFDPEDRDAYLCMGAFVETLSLAAANLGHRADFEPVFARSGSDLLVGRITIGPAPC
                     EAAIDPLAATISGRHTNRCEYDATPLPDELVAELKNLGAVMVSPAAMAGL"
     gene            complement(493..>1711)
                     /locus_tag="VGD55_06530"
     CDS             complement(493..>1711)
                     /locus_tag="VGD55_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870034.1"
                     /translation="AAELDESSGSSGTPYTWVRGEEELHEIHLTMAILARHLIDDGSG
                     TSRPVVTLNGFSMGAWATGTNVTAALKRLGVVKSTGPDADKMLSAMALLGTEPVFVIA
                     GYPPFLRRLLDAAREQQFDLSRYTMYGVVGGEGMSEALRNRLERTFASVYSAYGASDL
                     DIGVAAETALSIEVRRLAAAHPELAVALFGTTTRLPMVFQYDPSDYYVETIDHELVVT
                     VTRPALVSPRIRYNVHDAGGTLEYADVITACRALGLDPIAAAFARHPFRPFELPFLYV
                     HGRADSTISFMGANIYPEDVEQALGECADSDVLGAFALALIEIDGRDGTCDAVRPCVH
                     IEVLDPSRVGDGELSARLAGVVRDRLLGNSADFRSAVAEDLAAADIRVALHLEGAGPF
                     VGNATRIKRRYIV"
BASE COUNT          322 a          533 c          583 g          273 t
ORIGIN      
        1 caagccccgc cattgcggcg ggagacacca tgacggcgcc gaggtttttt agctccgcga
       61 cgagctcatc gggcaacggc gtcgcgtcgt actcacaacg gttggtgtgc cggccactga
      121 tcgttgcggc gagtggatcg atagcggcct cgcatggcgc ggggccaatc gtgatccggc
      181 cgaccagcag atcagacccg gaccgagcga agacgggctc gaagtcggcg cggtggccga
      241 ggttggctgc cgcgagcgac agggtctcga cgaatgcccc catgcacagg taggcatcgc
      301 gatcctcggg gtcgaagggc aggtagtcgg tgtagtcgta gtgcaactcg taacactggt
      361 cgtcaggacg cacctccaac cgccagggct gcgcgttgtg cgcggaaggt gcccgcagtg
      421 cggcggtcag aatcgcctcg tgctcgggtg tcagcatgtc gcgctcctcg tgggatgttg
      481 tggtgccggg tgtcagacga tgtaccggcg cttgatacgg gtggcgttgc cgacgaacgg
      541 gccagcgcct tcgaggtgga gcgcgacgcg aatatcggcg gcggcaaggt cttcggcgac
      601 agcgctgcgg aagtccgcac tgttgccgag caatcgatca cggacgacgc ctgcaagccg
      661 cgcactcagt tcgccgtcac caacccggct cggatcgaga acctcgatat gaacgcaggg
      721 ccggaccgcg tcgcaggtac catcgcgacc gtcgatctcg atgagcgcca aggcgaatgc
      781 gccgagcaca tcggagtcgg cacactcacc gagagcctgc tcgacgtcct ctggatagat
      841 gttcgcgccc atgaaagaga tcgtggagtc cgcccgaccg tgcacgtaga ggaacggcag
      901 ttcaaacgga cgaaacgggt gtcgggcgaa agctgcggcg atcgggtcaa ggccgagtgc
      961 ccgacacgca gtgatgacat cggcgtactc aagcgttccg ccggcgtcgt gaacgttgta
     1021 tcggatgcga ggagagacga gggccggccg ggtgaccgtc acaaccagct catggtcgat
     1081 ggtctcgacg taatagtcgc tcgggtcgta ctgaaagacc atcggaaggc gcgtcgtcgt
     1141 gccgaacaac gcgacggcaa gctccgggtg cgcggccgcc agccggcgga cctcgatcga
     1201 cagggcggtc tcggcggcaa ctccgatgtc gagatcgctc gcaccgtagg cggagtagac
     1261 cgaagcaaag gtgcgctcca gccggttgcg caacgcctcg ctcatcccct caccacccac
     1321 gaccccgtac atcgtgtatc gcgacaggtc gaattgctgc tcgcgcgcgg cgtcgaggag
     1381 acggcgcaga aatggtgggt agccggcgat gacaaagacc ggctcggtgc ccagcagtgc
     1441 catcgcgctc agcatcttgt cggcgtcggg gccagtcgac ttcacgacac cgagacgttt
     1501 caacgcggcc gtgacgttcg tcccagtggc ccacgctccc atggagaaac cgttcagggt
     1561 gacgacagga cgcgatgtgc cgctgccgtc atcgatcagg tgccgcgcaa ggatcgccat
     1621 cgtgaggtgg atctcgtgca gctcctcttc accgcgtacc caggtgtacg gcgtgcccga
     1681 cgaccccgac gactcgtcaa gctcggcagc g
//
LOCUS       DASWVF010000364         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_35075, whole genome shotgun sequence.
ACCESSION   DASWVF010000364 DASWVF010000000
VERSION     DASWVF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35075"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1683
                     /locus_tag="VGD55_06535"
     CDS             331..1683
                     /locus_tag="VGD55_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0870035.1"
                     /translation="MSDTDPRHRTHRRPTTLLAGTAVVIAAAAGAGAGGVALSKLGRT
                     AGATASSVAGTPVSTGASNGSGTSNASGTAGGSTSTGQPANWGWGYGGYGYSYGGSSG
                     STSSNSGSSATTEGSGGPSDVSTIAAGVDAGLVDINVTLGYQDAKGAATGIVLTSTGE
                     ILTNNHVVDGATAITVTDIGNGKTYRATVVGYDPTHDIAVLQLQGASGLNTAKLGDSS
                     KVTVGEAVVAIGNAGGTGGTPSAAGGAVTALNQQITAGDELGGTSEQLTGLIEVNADI
                     EAGDSGGSLVNSSGQVIGVDTAASSTNGTGQFAFDGSAISSDNQGFAVPINEALSIVK
                     QITSGHGTTTVHVGSTAFLGVELASTDSSGYGYYGYGATVSGATVAGVLDGGTAAGAG
                     LVAGDVITSLGGHAIDSASALSALMFSYHAGTSVKLGWTDSGGQSHTATVVLGSGPAT
                     "
BASE COUNT          319 a          610 c          591 g          262 t
ORIGIN      
        1 gcctcggctt tggcgctgtg tagggccaca cccacgccgt cccggcaatg atcagcagga
       61 ccagcacgat cagcccttga tgccgcaaag cggcttgaaa cgcggccaga atgagcgggc
      121 tgctgcctga cagtgagccg cccatcccct gcatggacgt caccctatgg gagcgctggc
      181 ggcgcggcga ctgcgcccgc agctttccca gcgtgcaccc agacgccggg aattcaggac
      241 cttcttagcg acgtttcacc gcacatccag ctatcggtcg cacgctgatc tcaagcggca
      301 cacatccgcc gactgacatc aggagtagcc atgagcgaca ccgacccccg tcatcgcacg
      361 catcgccgcc cgaccacgct gctcgccgga accgcggtag tcatcgcggc ggcggcaggt
      421 gcgggagcag gcggcgtagc cctttccaag ctaggccgga cggcgggggc gaccgcatcg
      481 tctgtcgctg gcaccccggt gagcacgggc gcctcgaacg gctccggcac ctcgaatgcg
      541 tctggcacgg ccggcggatc gacctccacc gggcagccgg ctaactgggg ttggggttac
      601 ggcggctacg gctactccta cggcggctcg tccggttcga cgtcgagcaa ctccggcagc
      661 tcagctacga ccgagggcag cggcggaccg tccgacgtgt caacgatcgc ggccggtgtc
      721 gacgctggct tggtcgacat caacgtcacg ctcgggtacc aggacgcaaa gggggcggcg
      781 accggaatcg tccttacgtc gaccggcgaa atcctgacca acaaccacgt cgtcgacggc
      841 gcgaccgcga tcaccgtgac cgacatcggc aacggcaaga cctacagggc gaccgtcgta
      901 ggctacgacc ccacgcacga catcgcggtg cttcagctgc aaggtgcgtc gggactgaac
      961 accgccaaac tcggcgactc gtcgaaggtc acggtcggcg aagcggtcgt tgcgattggc
     1021 aacgcgggcg gcaccggcgg cactccaagc gcggcgggcg gtgcggtcac cgcactgaac
     1081 cagcagatca ccgccggtga cgagctcggt ggcacgagcg agcagctcac cgggttgata
     1141 gaggtcaacg ccgacatcga agcaggcgac tcgggtgggt cgctcgtcaa ctcctccgga
     1201 caggtcatcg gcgtcgacac cgcggcgtcg agcacgaacg gcacgggcca gttcgcgttc
     1261 gacggctcgg cgatctcgtc ggacaaccag ggcttcgccg ttcccatcaa cgaggcgctc
     1321 tcgatcgtga agcagatcac ctcaggtcac gggacgacga cggtgcacgt cggctcgacc
     1381 gcgttcctcg gcgtcgaact cgcatcgact gacagcagcg gctatggcta ctacggctac
     1441 ggcgcgacag tcagtggcgc gacggtcgcc ggtgttctcg acggaggtac cgcagcgggc
     1501 gctggactcg ttgccggcga cgtgatcacc tcactgggcg gtcacgcgat cgactcggcc
     1561 tcagccctga gtgcgctgat gttcagctac cacgccggca cctcagtgaa gctcggctgg
     1621 accgatagcg gaggccaatc gcacaccgca actgtggtgc tcggcagcgg cccggcaacc
     1681 tgaccgctcg cgtactgaca ggtttacggc gatgaggacg gcggcgttgc tgggccgccc
     1741 ggcccgcccg acccgccgtt gaatatgcag ccgggagtgg tc
//
LOCUS       DASWVF010000365         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_188119, whole genome shotgun sequence.
ACCESSION   DASWVF010000365 DASWVF010000000
VERSION     DASWVF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188119"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGD55_06540"
     CDS             <1..195
                     /locus_tag="VGD55_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEY0870036.1"
                     /translation="EGQQRANRRSSETDEVVDDTAASDLKERQEKLNDDVDSILDEID
                     EVLEENAEEFVRSYIQKGGQ"
     gene            192..1037
                     /gene="prcB"
                     /locus_tag="VGD55_06545"
     CDS             192..1037
                     /gene="prcB"
                     /locus_tag="VGD55_06545"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEY0870037.1"
                     /translation="MTTDSSGRLPTAFHQPGVGSFADFLGMHAPDMMPGRRTLGPGTV
                     DAPHGTTIVAVTFQGGVVMAGDRRATMGNIIAQRDIEKVFQADEYSCVGIAGTAGLAI
                     ELVRLFQVELEHYEKIQGATLTLEGKANRLSMMIRGNLPMAMQGLAVVPLFAGYDQFV
                     PEPAKAGRIFSYDVTGGRYEEHSFHAVGSGSMFARGALKKFFRDDFTHDEAVRACVES
                     LYDAADDDSATGGPDLTRQIYPVVVACTADGFSRVTDDVLHDLVARVVDARMTRPGGP
                     AAPLS"
     gene            1089..1880
                     /gene="prcA"
                     /locus_tag="VGD55_06550"
     CDS             1089..1880
                     /gene="prcA"
                     /locus_tag="VGD55_06550"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEY0870038.1"
                     /translation="MSAPFYVSPEQLMKDRADYARKGIARGRSNVVLQYADGILFVAE
                     NASKALHKISEVYDRIAFAAVGRYNEFENLRVAGVRLADLTGYTYDRRDVTGRTIANA
                     YAQTLGAIFSGAGEKPYEVEIVVAEVGDEVDGDTMYRLTYDGSVADEHGFVAMGGQAE
                     HITGLLKERYVAGSSLTDALRLAVDVLTQAPDGEMRTLTAAQLEVAALVRSRARRAFR
                     RYVGPELETLLASSVPHQAESAASTPAEPAASDSDDPDVEADPSS"
BASE COUNT          341 a          655 c          631 g          288 t
ORIGIN      
        1 gaaggtcagc agcgcgctaa ccgccgctcg agtgagaccg atgaggtcgt cgacgacacc
       61 gccgcctccg atctcaagga gcggcaggag aagctcaacg acgacgtcga ctcgatcctc
      121 gacgagatcg acgaggtgct agaagagaat gccgaggagt tcgtgcgctc ttacatccaa
      181 aagggtggcc agtgacgacc gatagttcag ggcgtctgcc gacggcattt caccagcccg
      241 gcgtgggctc ttttgccgat tttctgggga tgcacgcgcc cgacatgatg cccggccggc
      301 gcacgttggg ccctggcacg gtcgacgcgc cgcatggaac cacgatcgtg gcggtgacgt
      361 ttcaaggcgg cgtcgtcatg gccggtgacc gccgggcgac gatgggcaac atcatcgccc
      421 agcgcgacat cgagaaggtc ttccaggccg acgagtactc ctgcgtcgga atcgccggga
      481 ctgcgggcct tgctatcgag ctggtgcggt tgtttcaggt cgaactggaa cactacgaga
      541 agatccaggg tgccacgctg accctcgagg gcaaggcaaa ccggctctcg atgatgatcc
      601 gcggcaacct gccgatggcg atgcaagggc tcgcggtcgt cccgttgttc gccggctacg
      661 accagtttgt gcccgagccc gcgaaagccg ggcggatttt ctcctacgac gtcaccggcg
      721 gccgctacga ggagcactcc ttccacgccg tcgggtcggg ctcgatgttc gcccgcggag
      781 cgctcaagaa gttcttccgc gacgacttca cgcacgacga agcggtcagg gcctgcgtcg
      841 agtcgctgta cgacgcggcc gacgacgact cagccaccgg tggccccgac ctcacccggc
      901 agatctatcc cgtcgtcgtc gcctgcaccg ccgacggatt cagccgcgtg accgacgacg
      961 tcctgcacga cctcgtcgcg cgggtggtcg acgcccgcat gacacgcccc ggcggccccg
     1021 ccgcaccgct gagctgacgc gcacccgccg cgcacccgac gaccaggacc ccagaccagg
     1081 agcactagat gagcgcgccg ttctacgtct cgccagaaca gttgatgaaa gaccgcgccg
     1141 actatgcccg caaggggatc gcgcggggtc gcagcaacgt cgtcctccag tacgccgacg
     1201 gcatcttgtt cgtggccgaa aacgcgtcga aggcgctgca caagatcagc gaggtctacg
     1261 accggatcgc gtttgctgcg gtggggcgtt acaacgagtt cgagaacctg cgcgttgccg
     1321 gcgtccgcct tgctgatcta accggctaca cgtacgaccg ccgcgacgtc accgggcgca
     1381 ccatcgccaa cgcgtacgcg cagactctcg gcgccatctt cagcggtgcg ggggagaagc
     1441 cgtacgaggt cgagatcgtc gtggccgaag tcggcgacga ggtcgacggc gacacgatgt
     1501 accggctcac ctacgacggc tcggtggccg acgagcacgg gttcgtcgcg atgggcggcc
     1561 aggctgagca catcaccggc ctgttgaaag agcgctacgt cgcagggtcc tcgctcaccg
     1621 acgccctgag gctggcagtc gacgtcctga cccaggcacc cgacggtgag atgcgcacgc
     1681 tgaccgcggc gcagctcgag gtcgcggcgc tggtgcgctc gcgggctcga cgggcgtttc
     1741 gccgctacgt cggccccgag ctggagacac tgctcgccag cagcgtcccc caccaggccg
     1801 aatcggcggc cagcacaccg gccgagccgg ccgcttcgga ctccgacgac cccgacgtcg
     1861 aggccgaccc aagctcctga gcgcagggca ggtccaagcc gcaggcgttt gccac
//
LOCUS       DASWVF010000366         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_111615, whole genome shotgun sequence.
ACCESSION   DASWVF010000366 DASWVF010000000
VERSION     DASWVF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111615"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGD55_06555"
     CDS             complement(<1..414)
                     /locus_tag="VGD55_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870039.1"
                     /translation="MLLIIVVLLAGGVVLAVTVPKVSRTAAPPLPRPTPTPSQAIITT
                     SQAPPTASLAPVSSPPEFVAWPTAATTGVPASAPPLQPMGQLVVTKNGATYTGLEING
                     CVDIMANDVTISQSVIHCNRAAPAVHVFPEFGNLVL"
     gene            complement(646..>2293)
                     /locus_tag="VGD55_06560"
     CDS             complement(646..>2293)
                     /locus_tag="VGD55_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HEY0870040.1"
                     /translation="LLVARPATGEVTMRKISDQFTRRLRGRAAQVVVVVGALAVVGVG
                     VPVGYIATASATTPATTQNLFTPQPTSATVSTDAKSVELGVNFVPAVSGQILGVEFLK
                     STHNVGTHTGSLWAPSGALLATGTFSKETASGWQTVLFAAPVTVSAGTIYTAGYHAAA
                     GHYDSTTAFFAAPRTSGNLGTHTAAGRKSYSAQTVMPTVDGGNNSNYWVDVIFAPTAD
                     APSPTSSPTTSPSPSPTVTVTPTPTPTASATPTPSPTGTASPTPTQTTTPTPTATPTG
                     SSGSSGTNFNYVLDSVGLKANIATKPNVWPNASNTGVPAGTTLKASGALVVTTAGTVL
                     SGLNISGCVDIQANNVTIKNSLITCGRSSVAIRVAHGFSGFLLQDSEVNGGGNAAGCI
                     GFGDFTLLRDNLHDCVDGVDGGGNAVVEYSYVHDLTRLTGTHNDTFQTVGGDHNLLVG
                     NTLQAYRASTSDLMNSSIQTGHLSSNLTDTLVEYNYMDGGNYTVNAGATSTNGYTIAG
                     YVFVGNQFGPDSRYGPVSELGTGITFDSSNVWAGTTTAVH"
BASE COUNT          400 a          664 c          783 g          446 t
ORIGIN      
        1 caagacgaga ttgccaaact cagggaagac gtgcactgcg ggcgctgcgc ggttgcagtg
       61 gatcactgac tgcgaaatgg tcacatcgtt cgccatgatg tcgacgcagc cgttgatttc
      121 gagcccggtg tacgtggcgc cgttcttcgt cacgacgagt tgacccatcg gttgtagcgg
      181 cggcgcggac gccggaacgc cggtcgtcgc ggctgtcggc catgcgacga actcaggggg
      241 gctcgagacc ggcgcaagac tcgcagtagg cggtgcttgc gacgtcgtga tgatggcctg
      301 cgagggcgtc ggtgtcggcc tcggcagtgg cggcgctgcc gtgcgcgaga ccttcgggac
      361 ggtgacagca agaacgacac cgccagcgag aagcaccacg atgatcagga gcaccgcgcg
      421 ccgactagtc ggacgaactg ctcgccgcca tcgtcgcttc ggggttgagt caccgggcgc
      481 accgtcgtca tgcattctat ggcgagtcta ggccaacgac tgcgagccgg tcggccgtct
      541 tgagagaacc gaggcaatcc caagcaaaga cggcccacga gtcgtggatc cttgggatcc
      601 acgtcgcggg ccgccttccg tgcgagttct acaagcgttc tactttcagt gaactgctgt
      661 cgtcgtgcct gcccagacgt tcgaactgtc gaaggtgatg ccggttccga gctccgaaac
      721 cgggccgtac ctggagtcgg gaccaaactg gttgcccacg aacacgtagc cggcgatggt
      781 gtagccgttc gtgctcgtcg ctcctgcgtt gacggtgtag ttgccgccgt ccatgtagtt
      841 gtactcgacg agcgtgtcag tcaggttcga cgacaagtgg ccggtctgga tgctcgagtt
      901 catgagatcg ctcgtcgagg cgcggtaagc ctgcagagtg tttccaacga gcaggttgtg
      961 atcgccaccc accgtctgga aggtgtcgtt gtgcgtcccc gtcaagcgag tcagatcgtg
     1021 cacgtagctg tactcgacaa ccgcgttgcc gccaccgtcg acaccgtcga cgcagtcgtg
     1081 caggttgtcg cgcagcaggg tgaagtcacc gaagccgatg caaccagcag cgtttccgcc
     1141 accgttgact tcgctgtcct gcaagaggaa gccgctgaag ccatgggcga cgcgaatcgc
     1201 gacactcgac cgaccacagg tgatcagcga gttcttgatg gtgacattgt ttgcctggat
     1261 gtcgacgcaa ccgctgatgt tcaggccgct caatacggtt cctgcggttg tcacgaccaa
     1321 cgcaccgctt gccttcagcg tcgtacctgc aggcacgccg gtgttgcttg cgttcggcca
     1381 cacattcggc ttggtggcga tgttcgcctt gagcccgacg ctatcgagca cgtagttgaa
     1441 gttcgttcct gacgaacctg acgaacctgt cggagtagcc gtaggagttg gtgtggtcgt
     1501 ctgtgtcggt gtcggtgagg cggtgccagt cggtgacggt gtgggtgtcg cggatgccgt
     1561 cggtgttggc gtcggcgtca ctgtgacagt cggcgacggt gaggggctgg tggtgggcga
     1621 cgacgtcggc gacggcgcgt ccgcagtcgg cgcgaagatc acgtcgaccc agtagttgga
     1681 gttgttgccg ccgtccaccg tcggcatgac ggtctgggcg gaatagctct tgcgaccggc
     1741 agctgtgtgg gtgccaaggt tgcccgaggt gcggggtgcc gcgaagaacg ctgtcgtgga
     1801 gtcgtagtga cccgccgcgg cgtggtaccc ggcggtgtag atcgttccgg cgctcacggt
     1861 gactggcgca gcgaacagga cggtctgcca acctgaagcg gtctccttgc tgaatgtccc
     1921 ggtcgccaac aaggcgccac taggggccca caacgagccg gtgtgagttc cgacgttgtg
     1981 agtgctcttc aggaactcca caccgagaat ctgaccggag acggcgggaa cgaagttgac
     2041 gccgagctcg accgacttgg catcggttga gacggtcgcc gaggtcggct ggggggtgaa
     2101 caggttctga gtcgtcgctg gggtcgtcgc acttgccgtt gcgatgtagc cgacgggaac
     2161 cccaactcca actacggcca gcgcaccgac gacgacgacc acttgggccg cacgtcctct
     2221 gagtcgccga gtgaactggt cggaaatctt gcgcatggtg acctctccgg ttgcaggtcg
     2281 agcgacgaga agt
//
LOCUS       DASWVF010000367        11237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_443079, whole genome shotgun sequence.
ACCESSION   DASWVF010000367 DASWVF010000000
VERSION     DASWVF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443079"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VGD55_06565"
     CDS             complement(<1..853)
                     /locus_tag="VGD55_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0870041.1"
                     /translation="MTDASTAADSRWWRDAIIYEIYVRSFADSDGNTVGDLKGITGRL
                     PQLADLGIDAVWLTPFYRSPMVDGGYDVADYRDVDPTLGTLADFDALLARAHELGLRV
                     IVDLVPNHTSDHHAWFKAALAAGPGSPERERYIFRDGRGSDGSEPPSDWIANFGGPAW
                     TQVADGQWYLHMFAPEQPDLNWSNPVVGEEFEGILRFWLDRGVDGFRIDVAHGMAKDM
                     SAPLPDLGIRSVDRQLRYEVVDHPLWDRDEVHDIFRNWRRILDEYSPPRIGVAEAWVM
                     PGRRARYV"
     gene            852..1988
                     /locus_tag="VGD55_06570"
     CDS             852..1988
                     /locus_tag="VGD55_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEY0870042.1"
                     /translation="MPAAYATRPATLSPPYVSLHVETDQERRLMEPVSLPANQPRQFY
                     LGGAEIEAFRGAAGTPASPSERAPEDWIASTVSKHGDSSVGGGATTLPDGRLLRDAVI
                     ADPLEYFGPAHVERFGANPALLVKLLDAGERLPVHCHPDRTFAGAHLASPFGKTEAWI
                     IIGTPGPDPHVYLGFNRDVEASELSEWFETQDSAEILSAMNRVPVGTGDAIFVPAGTP
                     HAVGAGVFIAELQEPTDFSVMLEWRDFGLADRHDGQLGMSAALSLECFNRKQMTDADL
                     ARYVRKAQPNSGGTARLFPDEADPFFQAEEIVVTDSIELSPQYAVLIVVGGTGSLTTA
                     QSEMALTRGSTVLIPYSAGATQLHAAAHEPLRAIRCLSGNASRD"
     gene            complement(2204..4708)
                     /locus_tag="VGD55_06575"
     CDS             complement(2204..4708)
                     /locus_tag="VGD55_06575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870043.1"
                     /translation="MPTRAADVDLDALLLDGRIARIRTVQAADADALRAMHDGVSADA
                     LYLRFFSLSRAVVPEEIQALTRAADDDHQALLAEIGGRVVAVATFERLNDDPTKAEIA
                     FLVDDSHRGRGVGMLLLEHLATAAVTAGVHSFVAETLASNSAMLHVFGDTGFPMISHY
                     NDGVVHIEIALEFDDEFRSAVDRREAHAEAISMRRVLAPLSVAVVGAGSDPAGLGHQI
                     LANIVRGGYRGTLYAVNQSAHRVSGVRAFTSLEALPTAVDLVVIAVPAAGVLDVARQA
                     ADHGAAGLVVISAGFAESNAAGEHLQQELVRVCRETGMRLIGPNCIGLANADPAISLN
                     ATASTTMPPAGGIGLMSQSGGVGFAALQYAARSGVGISSFVSAGNKADVSGNDLLCAW
                     EHDDETRVCALYLESFGNPRKFARIAARVSRRKPVVVVKAGRNVAGEPGLRLQRPGAA
                     RPDIAIDSLFEQAGVTRVDSLAELLDVASLFDLAPLPHGPRVAIVTNSGGPGLLAADA
                     CEAAGLDVVTLSAVTRHRLGAVVPTGTRVGNPVDLLSGADPVMFEAALRVLLDDPEID
                     AVISIYTPLEPGSATGIARAIATVQADVPGKPLLVCFLGVQTMPSELRASGGRPIAPY
                     YAFPEAAAHALGAAVRYTIWRSRPSGTQAVLADLDVARARAIVATALAEFPDGGWLDT
                     RDAAELLATHGVRLTERSSPERELATTPVAIGVVADPTVGPLVMVGLGGLASDMLADW
                     AFRLLPLTVEDARRQIRSLRGAPLLFDYDDAPPCDVVALEDMLLRVAELATNVPELAE
                     LDINPVLVSAGGAQAVKARVRLRPAPAEDPLIRRLR"
     gene            4923..5291
                     /locus_tag="VGD55_06580"
     CDS             4923..5291
                     /locus_tag="VGD55_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0870044.1"
                     /translation="MVESAQPVLDDEILERLERLGASVGEDLLGQLIVLFLADADTHV
                     IDLRRAVAASMPGDVVRLAHSLSGASANLGASNLALMCTGLETAGSVGDLVTGALLLD
                     ALEIELGQVRAALILRIPTT"
     gene            5288..6220
                     /locus_tag="VGD55_06585"
     CDS             5288..6220
                     /locus_tag="VGD55_06585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0870045.1"
                     /translation="MKVLVVDDDPTSRLIAQTAVESLGHDCVTACDGAQGWDALKSDQ
                     PDVVISDWMMPELTGIELFRTIRDGASDAYAYLILLTIESKLYKAIEGMRAGADDYLI
                     KPLDPDALRIRLIAAARVTALHDQLALQRTELEALNLTLTSMSLLDPLTGLGNRRALE
                     EEFEQLEARVARYGHRYCIAMLDIDNFKAFNDIYGHQAGDQALRDVAGQLRAEARKGD
                     ALYRYGGEEFLCILPEHSIPGGAIAVERMRVGVQSFAIPHSGNAEGILTISAGLSVLY
                     PGDTRPVVEVLKEADDALYRAKALGRNRVEGLAC"
     gene            6251..6652
                     /locus_tag="VGD55_06590"
     CDS             6251..6652
                     /locus_tag="VGD55_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870046.1"
                     /translation="MRTIVLLTAVALIPVGLLAASSIVLATHQVSNQVDNRVQATAGV
                     SSVVIGQQTTDLVALLHSYVTRRSLTAGLTAGATGNAAVDSSLASLAKAVPGISAAFV
                     TDMAGTSRNVYPAQPSVIGTNFAYREWYTGL"
     gene            complement(6715..7137)
                     /locus_tag="VGD55_06595"
     CDS             complement(6715..7137)
                     /locus_tag="VGD55_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870047.1"
                     /translation="MPMIDVYATVGTFSDKHTLAKDLANAVMRWEQVPPISLFRNNTA
                     AFVHELPADSLANAAGDSNYVRVQVLTPIGVLDRDKQLGVVRELTDIVATAAGDPSLT
                     DRTWVLISESPEGGWGINGHANTSADIVAAARRELAGE"
     gene            7224..7793
                     /locus_tag="VGD55_06600"
     CDS             7224..7793
                     /locus_tag="VGD55_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0870048.1"
                     /translation="MASDSRASMVRSAAALISSRGVSATSFADVVSDSGAPRGSIYHH
                     FPDGKRQLTEDAVRWTSGRVLAYLRSGPTDSPAQVLTRFIELWRQVVVSSGGSAGCVV
                     AGVAVDTVDDAPMLEIVRATFQSWVDALAEQLTAAGLPPDRAAPIAQITLAGMEGALI
                     LCRAEGSTAPLDIVADELVRLLPSTSLES"
     gene            7837..8820
                     /locus_tag="VGD55_06605"
     CDS             7837..8820
                     /locus_tag="VGD55_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0870049.1"
                     /translation="MPATETVYSIDAGGSRTTAEAARSGEQAQTWSRESFAIASAGRT
                     QAQAVLTSLLDDIRAWADPDESLGCIASSSMPVGQEAPAPRSLVEVIEKHAPRGRVVI
                     VNDVVPLLWSAGIAGVGIVLCSGTGSCVLGRDARGQLVKVGGHEHIISDQGSGYSLAR
                     TALRAAARDADSTGPRTKLRTEAETFFGRPLPALGRWLAELPRARTVVASFAPSVTLA
                     AAEGDDAAKAITQAEAAALVDMVQAAASALSLGELPIIGFAGGVLHGSSYFRELVEAE
                     LAARTLTRPDRTNLHLIDGISAGAQFALRLARNGDGSTLPLPDGEIISIES"
     gene            complement(8817..10298)
                     /locus_tag="VGD55_06610"
     CDS             complement(8817..10298)
                     /locus_tag="VGD55_06610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY0870050.1"
                     /translation="MTLQALDERALEILDSAVPKPRYDRRNVVVGIVHFGVGGFHRAH
                     QAMYVDALMNLGHALDWGICGVGTMSADEGMRDVMSAQDGLYTLVLKHPDGTLEPRII
                     GSIVEYLYAPDSPEAVIARLANPSTRIVSLTITEGGYNIHHVTGEFDAENPAVQRDLL
                     PGAIPLSVFGLVVEALARRRARGTPPFTVMSCDNIQGNGHVARRVFTGFARLRSPDLA
                     DWIEREVRFPSSMVDRITPVTTDDDRLLVERQLQIADAWPVVCEPFTQWVLEDEFVAG
                     RPPLEEVGVQLVESVEPYELMKLRLLNASHQALGYLGYLAGYRLVHEVCQDPLFVDFL
                     MRYMDDEATPTLGPVPGIDLAEYKRSLIGRFSNPYVRDTVSRLCAESSDRIPKFLLPV
                     IREQLRRDGEITRSTAVVASWARYAEGVDEQGEPIDVVDQLRDRLMAAARRQRLDPTA
                     FIADQGVFGDLASDTRFRSSYLAALSMLHKHGARGLLEQLALT"
     gene            complement(10301..11164)
                     /locus_tag="VGD55_06615"
     CDS             complement(10301..11164)
                     /locus_tag="VGD55_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0870051.1"
                     /translation="MSASTRYLADQQTKPITRLLLGVLTWVVVLMFFFPVFWMALTGF
                     KHEPDADTSTPHFLFKPTLDEYKSVWQGAGGLNGLRPYLEHSVLITVLSTVIVLVLAL
                     PAAYALSIRPVPKWRDVLFFFISTKMLPVVGSIIPLYVIARDLHVLDHQRLLVVLYTS
                     MNLPIAVWMLRSFLLEVPAEVIEASRVDGAGLLRQLRSVILPVISPGVAATALICFIF
                     AWNEFFLAVNLTTIRGATVPIYLVGFVQPEGPFLAHLSAAATVACLPVLLAGWAAQDK
                     LVRGLSMGAVK"
BASE COUNT         2107 a         3652 c         3592 g         1886 t
ORIGIN      
        1 gcacgtagcg ggcccggcgg ccgggcatca cccaggcttc ggccacgccg attcgcggcg
       61 ggctgtactc gtcaagaatc cgtcgccagt tgcggaagat gtcatgcacc tcgtcgcggt
      121 cccacaacgg gtggtcgaca acctcgtagc gcagctgccg gtcgacggat cgaataccca
      181 ggtctggcaa cggcgcactc atgtctttcg ccatcccatg ggcaacgtcg atgcggaagc
      241 catcgacgcc acggtcgagc cagaaacgca ggatcccttc gaactcctcg cccacgaccg
      301 ggttcgacca gttcagatcg ggctgctcgg gcgcgaacat gtgcaggtac cactgcccgt
      361 cggcgacttg ggtccacgcc ggcccgccga agttcgcgat ccaatcgctc ggcggctcgc
      421 taccatcact gccgcgaccg tcccggaaga tgtaccgctc tcgttccggt gagcccggac
      481 cggctgccaa tgcagctttg aaccaggcgt ggtgatctga ggtgtggttg ggcaccaggt
      541 cgacgatcac gcgcagaccg agctcatgcg cgcgagcgag cagggcgtcg aagtcggcaa
      601 gcgtgccaag agtcgggtcg acgtcgcggt agtcggcaac gtcatagccg ccgtcaacca
      661 tgggcgagcg gtagaacggc gtgagccaga cggcgtcgat cccgaggtcg gcgagttgcg
      721 gcagccgccc ggtgattccc ttgaggtcgc cgacggtgtt gccgtcggaa tcggcgaagc
      781 tccgcacgta gatctcgtag atgatggcgt cgcgccacca gcgcgagtcg gcagcagttg
      841 acgcgtcggt catgcccgca gcgtacgcaa cccggcccgc tacgctcagc ccgccctacg
      901 tgagcttgca cgtcgagacc gaccaggagc gccgtctcat ggagccagta agcctgccgg
      961 ccaaccagcc gcgccaattc tacctcggag gcgccgagat cgaggccttc cgaggcgcgg
     1021 ccggaacacc ggctagtccg agcgagcgcg cgcctgagga ctggatcgcg tcaaccgtgt
     1081 ccaagcacgg cgacagcagc gttgggggcg gcgccacgac tctgcccgac ggccgcctgt
     1141 tgcgcgacgc cgtgatcgct gacccgttgg agtacttcgg ccctgcccat gtcgagcgat
     1201 tcggcgccaa cccggcgctg ctggtgaagt tgctcgacgc cggcgagcgg ttgcccgttc
     1261 attgtcaccc tgaccgcacg ttcgcagggg cccacctcgc gtcgccgttc gggaagaccg
     1321 aggcgtggat catcatcgga acaccaggcc ccgacccgca cgtctacctc ggcttcaacc
     1381 gcgacgtcga ggcgagcgaa ctctcggagt ggttcgaaac ccaagacagt gcggaaatcc
     1441 tgtcggcgat gaaccgggtg ccggtcggca ctggcgatgc gattttcgtg cccgccggca
     1501 caccacacgc tgtcggcgcc ggagtgttca tcgccgaact tcaagaaccg accgactttt
     1561 ccgtgatgct cgagtggcgc gacttcgggc tggctgatcg ccacgatggc caactcggca
     1621 tgagcgcggc attgtcgctt gaatgtttca accgcaagca gatgacggac gccgatctcg
     1681 ctcgctacgt ccgcaaggcg cagccgaact ctggcggcac cgccaggttg tttcccgacg
     1741 aggccgaccc gttctttcaa gccgaagaga ttgtggtcac cgactcgatc gagctctctc
     1801 cgcaatacgc agtgctgatc gtggtcggcg gcactggcag cctcacgaca gcccaatcgg
     1861 aaatggccct cacccgcggc tcgacggtgc tgatcccgta ctcggccgga gcaacgcagt
     1921 tgcacgcagc tgcgcacgag ccgttgcgcg cgatccgttg cctgtcaggc aatgcgagcc
     1981 gcgactaagc gccgcgggca ggcgaagcgg cgttctgtgt gggtgcatcg ccgagtgttc
     2041 acaaacggct gtctttcgca gggatttcgc gccgattgcg cacactcggc gagcgagcgg
     2101 tcgcgggcaa ggggtcgctg accgagagtg cggcggaccc ggtcgccagc tgaggttttt
     2161 gtaggggacc gtcgggacgt ccccgtggtc aggccaacca cagtcagcgg agccggcgga
     2221 tcaacggatc ctcagcgggc gccggtcgca gccgaacgcg cgcctttact gcttgggcac
     2281 cacccgcaga caccaacacc gggttgatgt cgagttcggc gagctcggga acatttgtcg
     2341 ccaactcggc tacccgcagc agcatgtcct ccagcgcgac gacatcgcac ggcggcgcat
     2401 cgtcataatc gaagagcagc ggcgccccac gcagtgaccg aatttggcgt cgagcgtcct
     2461 cgacggtcag cggcaacaac cgaaatgccc agtcggccag catgtcactg gcgagacctc
     2521 caagacccac catgacgagc ggaccgacgg tcgggtcggc tacgacgccg atcgccaccg
     2581 gggtcgtcgc aagttcacgt tcggggcttg aacgttcggt gaggcgcacc ccgtgcgtcg
     2641 cgagcaactc ggctgcgtca cgcgtgtcga gccagccgcc gtcgggaaac tcggcgagtg
     2701 ccgttgcgac gatcgcccgc gcccgcgcta cgtcaagatc cgcgagcacg gcctgcgttc
     2761 ctgatgggcg cgaccgccat atcgtgtagc gaacagcagc gccaagcgcg tgcgcggccg
     2821 cttcgggaaa tgcgtaatac ggcgcgatcg gacgacctcc agaagcgcgc aactccgacg
     2881 gcatcgtctg gacgccaagg aaacacacga ggagaggttt ccccggcaca tcagcctgca
     2941 cggtcgcaat cgcacgggcg attccggtcg ctgatcccgg ctccaagggg gtgtaaatgc
     3001 tgatgaccgc gtcgatctcg gggtcgtcga gcagcacccg cagggctgct tcgaacataa
     3061 ccggatccgc ccccgagaga agatcgacag gattgccgac gcgggtacct gtaggcacaa
     3121 ctgcgccaag gcgatgccgc gtcaccgcac tcaaggtgac gacgtcgagt ccggcggcct
     3181 cacaggcgtc ggcggccagc aggcctgggc caccgctgtt ggtgacgatg gcgacgcgcg
     3241 gcccatgggg cagcggcgcg aggtcgaaaa ggcttgctac atcgagcaac tctgcgaggg
     3301 aatcgacccg cgtgacgccc gcttgttcga agagactgtc gatcgcgatg tcgggccttg
     3361 cggcgcctgg tcgctgcaga cgcagacccg gttcgccagc gacattgcga cccgctttca
     3421 ccacgacaac gggcttgcgc cggctcaccc gtgcggcgat ccgcgcgaac ttgcggggat
     3481 tgccgaacga ctcgagatac agcgcgcaga cgcgtgtctc gtcgtcgtgc tcccacgcgc
     3541 acaagaggtc gttgccggag acatcggcct tgttgccggc cgacacgaag ctggagattc
     3601 cgacgcccga gcgcgcagcg tattgaagcg cggcgaaccc gacgccaccc gactgagaca
     3661 tgagcccgat accgcccgca ggcggcatgg tcgtgctggc cgtcgcattc aaagaaatcg
     3721 ctgggtcggc gttcgcaagg ccgatgcagt tgggcccgat cagccgcatg cccgtctctc
     3781 ggcacacgcg cacgagctcc tgctgaaggt gctcaccggc cgcattcgat tcggcaaacc
     3841 ccgcggagat caccacaaga ccagccgccc cgtgatcggc agcctgccgc gcgacgtcaa
     3901 ggacaccggc agcgggcact gcaatgacga ccagatcgac agctgtcggc aacgcttcga
     3961 gcgacgtgaa tgctcgcacg cctgacaccc ggtgcgccga ttgattcacc gcgtacagcg
     4021 tgccgcgata tccgccccgc acgatgttcg ccaggatctg atgtccaaga cccgccggat
     4081 cagatcccgc cccgacaacc gcaaccgaca gcggagcaag cactcgtcgc atcgatattg
     4141 cctcggcatg agcttcgcga cgatcgacgg cgctgcgaaa ctcgtcgtcg aattcgaggg
     4201 cgatttcgat gtgcacgacg ccatcgttgt agtgagagat catcggaaat ccggtgtcgc
     4261 cgaagacgtg aagcatcgcg gaattgctcg ccagggtttc agcaacaaat gagtggacgc
     4321 cggcggtgac cgccgcagtc gcgaggtgct cgaggagcag catgccgact ccgcggccgc
     4381 gatgggaatc gtcgacgaga aaagcgatct cggccttagt agggtcgtcg ttgagccgct
     4441 cgaacgtggc taccgcgaca actcggccgc caatctcggc gagcaacgcc tggtgatcgt
     4501 cgtcggcagc cctggtaagt gcctggatct cctcaggaac gacggcgcgc gacaacgaga
     4561 agaatcgcaa gtaaagcgcg tccgccgaca ccccatcgtg catggcccgc aaagcatccg
     4621 cgtcggctgc ttgcaccgtt ctgatccgcg cgatacggcc gtcgagcagc aaggcgtcga
     4681 ggtcgacgtc ggccgcgcgg gtaggcatcg tcgatctcct ctccaggtgt ttgacgctaa
     4741 cgagcgcgcc cgccgaggta ccagcgacgt caggcccgcg tggcagggcc gttggtcccg
     4801 aagcccggca taagggactt cggacctgtt gtcccgggct cgcctcaggt tcgtcgtccg
     4861 acggtcgatg ggcgacctat gtcgcttgac acagcggagc cgatctcgaa cgagctgaac
     4921 ccgtggtcga gtcggcccaa ccggtgctcg acgacgagat cctcgagcgg ctcgagcgcc
     4981 tgggcgcatc agttggagaa gacctcctcg gccaactcat cgtcctcttc cttgctgatg
     5041 ccgacacgca cgtcatcgac ttgcggcgtg ccgtcgcggc aagcatgccc ggagacgtcg
     5101 tgcgactggc gcactccctg agcggagcga gcgcgaacct cggagcctcc aacctcgccc
     5161 tcatgtgcac gggattggag accgcaggtt cggttggcga cctcgtgacc ggggcactcc
     5221 tcctcgatgc gctggagatc gaactgggtc aggtccgcgc agcgctcatc ctgcggattc
     5281 ccacgacatg aaggtgctgg tcgtcgatga cgaccccaca tcgcgactca ttgctcaaac
     5341 ggccgtagaa agcctcggcc acgactgcgt cactgcctgc gacggtgcac agggttggga
     5401 cgcccttaaa tcagaccagc ccgacgtcgt tatcagcgac tggatgatgc cagaactgac
     5461 agggattgaa ttgttccgca ccatccgcga cggggcatcg gatgcgtatg cctacttgat
     5521 tctgttgaca attgaatcga agctttacaa ggcgatcgaa gggatgcgag caggcgccga
     5581 cgactatctg atcaagccac tcgacccaga tgcactgcgg attcgcctga ttgctgcggc
     5641 tcgggtgact gcgttgcacg accagctcgc gctccagcgc accgaactcg aagctctgaa
     5701 tctaaccctg acctcgatgt cgctgttgga tccgcttacc gggctaggaa accgccgagc
     5761 tctcgaggag gagttcgaac aactcgaggc ccgagtcgcc cgctacgggc accgatattg
     5821 catcgcgatg ctggacatcg acaacttcaa agcgttcaac gacatctacg gtcaccaggc
     5881 cggcgaccag gctctgcgcg acgttgctgg ccagctaaga gcggaggcga gaaagggcga
     5941 cgcgctctac aggtacggcg gcgaagagtt cttgtgcatc cttcccgagc attcgattcc
     6001 cggcggggcc atcgcggtcg agcgaatgcg agtcggtgtt cagtcatttg cgatccctca
     6061 ctccggcaac gcggaaggca ttctcaccat cagcgctggc ctgtccgtcc tgtacccagg
     6121 agacacacga cccgtggtcg aggtactcaa ggaagccgac gacgccctct accgagccaa
     6181 ggcattaggg cgcaatcgag tcgaaggctt ggcgtgctag gtgaaatcct cccgatcaac
     6241 gcgcgcttcc ttgcgcacca tcgtgttact aaccgcagtg gcgttgattc ccgtcggtct
     6301 tcttgcagca agcagcatcg tcttggccac ccaccaggtc tctaaccagg tcgacaatcg
     6361 tgtgcaagcg acggccggtg tcagttcggt ggtgatcggc cagcagacaa cggatctcgt
     6421 cgctctcttg cacagctacg tgacccgacg ctcactgacc gcggggctca ccgcaggcgc
     6481 aaccggcaac gccgcagtgg actcgagcct ggcgagcctt gcgaaggctg tccccggcat
     6541 ctctgccgcc ttcgtcaccg acatggcggg cacatcgcgc aatgtctacc cggcgcaacc
     6601 gagcgtgatc ggcaccaact tcgcctaccg cgagtggtac acgggcctct gaccaagccg
     6661 atcaaggccg aggatctcga cgccaagctg acgtactggc tccagcagta agccctactc
     6721 ccccgcgagt tcgcgacggg ctgccgcgac gatgtcggcg ctggtgttcg cgtgcccgtt
     6781 gatgccccag ccaccctcag gggattcgct gatgagcacc cacgtgcggt cggtcaggct
     6841 cgggtcgccc gccgcggtcg cgacgatgtc ggtcagctcg cgcacaaccc cgagctgctt
     6901 gtcgcgatca aggacgccga tcggagtcag cacctgcacg cgaacgtagt tgctgtcgcc
     6961 cgcggcgttg gccagcgaat cagccggcag ttcgtggacg aacgcagcgg tgttgtttcg
     7021 aaagagcgag atcggcggaa cctgctccca ccgcatgacg gcgttggcaa ggtctttcgc
     7081 gagggtgtgc ttgtcactga aggtcccgac tgtggcgtac acgtcgatca tgggcattgg
     7141 agctctcctc gaaatatgat gtccgtcata gagcgtgccc agtgtgcgtt atgacgatcg
     7201 tcatagtcaa gagggagcca gcggtggcca gcgacagccg ggcgagcatg gtgcgcagcg
     7261 ccgccgcgct cattagctcg cgaggcgtca gcgcaacctc gtttgccgac gtggtcagcg
     7321 acagcggcgc tccgcgcggt tcgatctacc accacttccc cgatgggaag cggcaactga
     7381 cggaggacgc cgttcggtgg acctccggtc gggtgctcgc gtatctgcgc tcagggccga
     7441 ccgactcacc cgcgcaggta ctgacgcgct tcatcgagct atggcgtcag gtcgtcgtga
     7501 gctctggggg ctcagccggt tgcgtcgtgg ccggagttgc cgtcgacacg gtcgacgacg
     7561 cccccatgtt ggagatcgtg cgcgcgacgt tccagtcgtg ggttgacgca ctcgccgagc
     7621 aactgactgc tgccggcctg cctcctgatc gagcagcgcc aatcgcccag atcacgttgg
     7681 cgggcatgga aggtgcactc atcttgtgcc gtgccgaggg cagtactgcg ccgctcgaca
     7741 tcgtggccga tgaactcgtg cggcttctgc catcgacaag ccttgagagc tgactgggcg
     7801 ggcggttgtt gccgtagtca cgtctattct gtccccatgc ccgcgaccga gaccgtctac
     7861 agcatcgacg ccggcggctc gcggaccacc gcagaagcag cgcgctcagg tgagcaagcg
     7921 cagacgtgga gtcgcgagtc gtttgcgatc gcctcggcag gtcgcacgca ggcgcaggct
     7981 gttctcacgt cacttctcga cgatatccgc gcgtgggctg accctgatga gtcgctcggc
     8041 tgtatcgcgt cctcgtcgat gccggtcggg caagaggcgc ccgcccctcg atcccttgtc
     8101 gaggtgatcg agaagcacgc tccccgcggc cgggtggtca tcgtcaacga cgttgtccca
     8161 ctcctttggt cggctggaat tgcgggcgtg ggcatcgtgt tgtgcagtgg caccggcagt
     8221 tgcgtactgg gtcgcgacgc gagaggtcag ttggtgaagg tcggtggcca cgaacacatc
     8281 atttccgacc agggcagcgg ctacagcctc gcccgcacgg ccttacgcgc ggccgcccgc
     8341 gacgccgaca gcaccggccc tcgcactaaa ttgcgaaccg aggccgaaac attctttggt
     8401 cgtccgctcc ccgcactcgg gcgatggctc gcagagttgc ctcgcgcccg tacggtcgtc
     8461 gcaagcttcg ccccttccgt cacgttggcg gccgcagaag gtgacgacgc ggccaaggcg
     8521 atcacgcaag ccgaagcagc cgcgctcgtc gacatggtgc aggccgcggc ttcagcgttg
     8581 agcctcggcg agctgccgat catcgggttc gccggcggcg tcctgcacgg cagttcgtac
     8641 tttcgagaac tggtcgaagc cgagttggcg gcgcgcacac tcactcgccc cgaccgcacc
     8701 aatctccacc ttatcgatgg catcagcgcc ggcgcgcagt tcgccttgcg gttagcacga
     8761 aacggcgacg ggtcaacgct gccactgccg gacggcgaga tcatcagcat cgagagctag
     8821 gtcagagcta gctgttccag gaggccgcga gcgccgtgct tgtgcagcat cgacagtgct
     8881 gcgagatagc tcgaccggaa ccgggtgtca ctcgcgagat ccccaaaaac accttgatca
     8941 gcgataaatg cggtcgggtc taggcgctgt cggcgcgcag ccgccatcag gcgatcgcgc
     9001 agttggtcaa ccacgtcgat cggctcgccc tgctcatcta ccccttctgc atagcgcgcc
     9061 cacgacgcca cgactgcggt cgatcgggtg atctcgccgt cacggcgaag ctgttcgcgg
     9121 atgacaggaa gtaggaactt ggggatgcga tcggacgact ccgcgcacaa gcgcgacacg
     9181 gtatctcgca cgtacgggtt agagaaccgt ccaataaggc tgcgcttgta ttccgccagg
     9241 tcgatgcccg gtacagggcc aagcgtcggc gtcgcctcgt cgtccatgta acgcatcagg
     9301 aaatcgacga acaacggatc ctggcagacc tcgtggacga gtcggtaacc cgcgaggtag
     9361 ccgaggtatc cgagggcctg gtggcttgcg ttcaacaacc gcaacttcat cagttcgtag
     9421 ggttccacgc tctcgacgag ctgcaccccg acctcctcga gaggcggccg accggcaacg
     9481 aactcgtcct cgagaaccca ctgcgtgaac ggctcgcaca cgaccggcca cgcatcggca
     9541 atctgcagct gccgttcgac cagcagtcgg tcgtcatcgg tcgtgaccgg cgtaatgcgg
     9601 tcgaccatgg acgacgggaa gcgcacctcg cgttcgatcc agtcggcgag gtcagggctt
     9661 cgcaatcgcg caaagcccgt gaagacgcga cgggcgacgt gaccgttgcc ctggatgttg
     9721 tcgcacgaca tgaccgtgaa gggcggcgtc ccgcgtgcgc gtcgacgagc gagggcctcg
     9781 acgacgagcc cgaagaccga cagtgggatt gcgccgggca gcaaatccct ttgcaccgca
     9841 gggttttcgg catcgaactc acccgtcaca tgatgaatgt tgtagccccc ctcggtgatc
     9901 gtgagcgaga cgatccgcgt cgaagggttg gccagcctgg cgatgaccgc ctcgggtgaa
     9961 tcgggggcgt acaggtactc gacgatcgag ccgatgatgc ggggctcaag tgtcccgtcg
    10021 gggtgtttca gaaccagcgt atagagaccg tcttgggccg acatgacgtc gcgcatgccc
    10081 tcgtcggcac tcatcgtgcc gacaccgcaa atgccccagt cgagcgcgtg tccgagattc
    10141 atcaacgcgt cgacgtacat cgcttggtgc gcgcgatgaa agccgcccac gccgaagtga
    10201 acaatcccga ccaccacgtt ccggcggtcg tagcgcggct ttggcacggc actgtcgagg
    10261 atttcgagcg cgcgttcgtc aagcgcctgc agagtcactg ttacttcact gcgcccatcg
    10321 acaacccgcg cacgagcttg tcttgagctg cccagccagc gaggagcacc ggcaggcagg
    10381 cgacggtcgc ggccgccgac aggtgggcca ggaacgggcc ctccggttgc acgaacccga
    10441 caaggtagat gggcaccgtc gcaccgcgga tcgtggtcag gttgaccgca aggaagaact
    10501 cgttccacgc gaagatgaaa cagatgagag ccgttgccgc aacgccagga ctgatgacgg
    10561 gcaagatgac cgaacgcaac tggcgcagca gcccggcgcc gtcgacccgc gatgcctcga
    10621 tgacctcggc cggcacctcg agaaggaagg accggagcat ccacaccgcg atcggcaggt
    10681 tcatcgaggt gtacagcacg accagcagtc gctggtggtc gagaacgtgg agatcgcgcg
    10741 ctatgacata cagcgggatg atcgatccga cgaccggcaa catcttggtc gaaataaaga
    10801 agaacaagac gtcgcgccac ttcgggaccg gtcggatcga cagtgcatag gcggccggga
    10861 gcgcgagcac caggacgatc acggtcgaca ggactgtgat cagcactgaa tgctcaagat
    10921 agggccggag accgttaagg ccgcctgctc cctgccatac cgacttgtac tcgtcgagcg
    10981 tcggcttgaa gagaaagtgc ggcgtgcttg tatcggcgtc gggctcgtgt ttgaaaccgg
    11041 tcagtgccat ccagaaaacg gggaagaaga acatcaacac gacgacccat gtcaagacgc
    11101 cgagtagcaa cctcgtgatg ggcttggttt gctggtcggc gaggtatcgc gtagacgcgc
    11161 tcatcgccct cgcatccctt cgtcgctgaa cagactcgag atcatccgca acgccaaggt
    11221 cgcgatgaga atggtcg
//
LOCUS       DASWVF010000368         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_366318, whole genome shotgun sequence.
ACCESSION   DASWVF010000368 DASWVF010000000
VERSION     DASWVF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366318"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1025..1321)
                     /locus_tag="VGD55_06620"
     CDS             complement(1025..1321)
                     /locus_tag="VGD55_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870052.1"
                     /translation="MSIQRTIHGNMVTITFVLDDESNTAPASVVGSFNDWTPGAHPFE
                     RSHDGGRAVTIEVPTSLPLHFRYLAADGVWSDDEDADEVTDEGSFIWAAAPPQA"
     gene            complement(1413..3476)
                     /gene="glgX"
                     /locus_tag="VGD55_06625"
     CDS             complement(1413..3476)
                     /gene="glgX"
                     /locus_tag="VGD55_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0870053.1"
                     /translation="MTLPLAGLPFPLGATTTEGGTNFAVASEIADAIEVCLFAEDGSQ
                     TVVELPQRTAHVFHGMLPAIGAGQRYGLRVHGPWEPSSGLRCNPAKLLLDPHATAIDG
                     NIEWGEDVFGHLFDDPERRNDVDSAHAMPKCVVTDRQFNWSGDRAPRTPLAETVIYET
                     HVKGFTKRHPDIPSDIQGTYAGLAHPAAIGYLSELGVTAVELLPVHQFVQDSQLLDRG
                     LRNYWGYNSIGFLAPHGGYSSAGTAGGQVDEFKAMVKALHAAGLEVILDVVYNHTAEG
                     NHLGPTLSMKGIDNPAYYRLVEGDEAHYFDTTGTGNSLQVGHPAALRLIMDSLRYWVT
                     DMHVDGFRFDLASTLTRQNGEIDIHSAFLNLVHQDPVLAPVKLIAEPWDTAGYQVGGF
                     PARWSEWNGKYRDDVRDFWRGEGNLGNLGLRLTGSSDIYASDRRTPTASINFVTAHDG
                     FTLADLTAYNEKHNEANGEDNNDGETNNSSWNCGVEGPTDDAEIKDLRARQRRNLLGT
                     ILLSAGVPMILGGDEIGRTQDGNNNAYCQDNELSWYDWNDVDENLLAFTKTAVKLRRE
                     NPALHPRDYLRGRGGLPAQMVLYRSDGIEMSDDDWQDGGRRALTVALDRRRIQDADGD
                     TTRDRFLLFVNGHDHPLDFTIPPGGHHDWRVVLTTGSPEPVGPVKSGDNLSVRDRSML
                     LLHSA"
BASE COUNT          633 a         1179 c         1207 g          707 t
ORIGIN      
        1 ggctaaccgc gtcagttctt cgtgggcggc ctgccgacgc tcagcggcct gcagtgcggc
       61 gatcgcacgc tgccacgcct cgaaaggctc cgacgcgctc cgcgcgtcag gcacaacaga
      121 catgatcaca ctctccagag ctcgctaccc aggggctaga ggcacggtct gctggctgaa
      181 accacaccaa ttttacaccc gcgttcgagc cgcagcattt gggcacaacc gggtcgtgac
      241 cgctacagtc ccgacgctcg cctgcgtgcc ctacgccgcg tgcgactgcg ccggcggctc
      301 cccctgccaa cagcgcagcc gtgccgggtg catcacattc gccgctgaga aatgtccgac
      361 gatgccccac agtgaagcgc tccgcgactg ggtccggcgc agatccgcta cgacggacca
      421 gcgcttcgcg gtccgctcta caccttgcag caactccgcg aactcgtgaa cgcagatctc
      481 cacggactcg acgccgtcca acccttctca actatgcaga caccgagagc cgaactcgcc
      541 gagccctacc acgaccgcaa cggccacgag caggtcgcaa ggtgaacgcg tcctcatcgg
      601 ccgcgtccgc cgaaccccaa actatcgacg tgctcgcgca aacagcctcc ggcgcgcaag
      661 taaactgtta ctgcccatta caagtcgaag gaccgtgcac cccgctgccg cgaccagccc
      721 cggcaatgcg gacgtcccgt tcgatatctc caccgcgacg tacggcttca cagttgaagt
      781 cgaggacgcc gcttctctga gcgcgctcca ccagcttcac gctggccccg tgagcatgtg
      841 cggccttcag tgcgcgccac gcgggtagcc gcaacgttgc ctggtttgag tgagtcgcgg
      901 gacgtcgagc ccgtcgatgc gctcggccta cgcaacctgc acccatcccg accgagagtt
      961 ccggcaaggc ccaatgtctg aggtgtgttc cgccgttccg tcaacaggac gcaccggcgg
     1021 aaacttacgc ctgcggtggt gcggcggccc agatgaacga gccttcgtcg gtgacctcgt
     1081 cggcgtcttc gtcatcggac cagactccgt cggcggcgag gtaacggaag tgcaacggca
     1141 gagacgttgg cacctcgatt gttaccgcgc gaccgccatc gtgggagcgc tcaaatgggt
     1201 gcgcacccgg cgtccagtcg ttgaacgagc ccacgacgga cgccggtgca gtgttggact
     1261 cgtcatcgag gacgaatgtg atcgtgacca tgttgccgtg gatggtgcgc tgaatgctca
     1321 tctcgctccg ttctgttcgc ggaatgtcct tcggtttcga caaggcgcgc cttctgtatg
     1381 ccgcctgatg aggaggcgcg ggcccggaaa gactaggccg agtgaagcaa gagcatggac
     1441 cggtcgcgca ccgataggtt gtcgccgctc ttgactgggc cgactggctc ggggctaccg
     1501 gtggttaaga cgacacgcca gtcgtggtgg cctccaggtg ggatggtgaa gtcgagcggg
     1561 tggtcgtgcc cgttgacgaa cagaaggaaa cggtcgcggg tcgtgtcgcc gtccgcgtct
     1621 tgaatgcgtc ggcggtccag ggcgacggtg agtgctctcc tgccgccgtc ttgccaatcg
     1681 tcgtctgaca tctcgatgcc gtccgagcgg tagagcacca tctgggccgg cagaccaccg
     1741 cggccgcgta ggtagtcgcg tgggtgcaat gccgggtttt cacgccgcag tttcaccgcg
     1801 gtcttggtga acgccagcag gttctcgtcg acgtcgttcc agtcgtacca ggacagttca
     1861 ttgtcttggc agtaagcgtt gttgttgccg tcctgggttc ggccgatctc gtcgcccccg
     1921 aggatcatcg gcacgccggc ggacagcaat atggtgccga gcaggttacg gcgttgccgc
     1981 gcacgcaggt ccttgatctc ggcgtcgtcg gtcgggcctt cgacgccgca gttccaggag
     2041 ctgttattcg tttcgccgtc gttgttgtct tcgccgttag cctcattatg cttctcgttg
     2101 taggccgtga gatcggcgag ggtaaatccg tcgtgcgccg tgacgaagtt gatgctggcg
     2161 gttggggtgc ggcggtcgga ggcatagatg tcgctgctgc ctgtgagccg aagcccgagg
     2221 ttgccgagat tgccttcgcc gcgccagaag tcgcgaacgt catcgcggta tttgccgttc
     2281 cactctgacc agcgggcggg gaagccaccg acttgatagc cggcggtgtc ccacggttcg
     2341 gcgatgagct tcaccggggc gagcaccggg tcttggtgga cgagattgag gaaggcacta
     2401 tggatgtcga tctcaccgtt ctgccgagtg agggtgctgg ccaggtcgaa ccggaagccg
     2461 tcgacatgca tgtcggtcac ccagtaccgc aacgagtcca tgatgagtcg cagcgctgcg
     2521 gggtggccga cttggaggct gttcccggtg ccggtggtgt cgaagtagtg ggcttcgtcg
     2581 ccttccacga gccgatagta ggcagggttg tcgattccct tcatcgacaa cgtggggcct
     2641 aggtgatttc cctccgcggt gtggttgtac acaacgtcga ggatgacttc gagcccggcc
     2701 gcgtgcaggg ctttcaccat cgctttgaac tcatccacct ggcctccggc ggtacccgcc
     2761 gagctgtatc cgccatgggg ggccaggaaa ccgatcgagt tgtagcccca atagttacgc
     2821 agtccccggt cgagcagctg cgaatcctgt acgaactgat gcaccggcag caactccacc
     2881 gccgtgacgc ctaactcact caggtagcca atcgcggctg ggtgtgccag gcccgcgtag
     2941 gtcccttgaa tgtcgctggg gatatcgggg tgccgtttag tgaacccttt gacgtgtgtc
     3001 tcatagatga ccgtttcggc gagcggggtt cggggtgccc tgtcaccgga ccagttgaat
     3061 tggcgatcgg tcaccacgca cttaggcatc gcgtgcgccg aatctacgtc gtttcgacgc
     3121 tcggggtcgt cgaataggtg gccgaagacg tcctcgcccc actcgatgtt gccgtcgatg
     3181 gctgtggcgt gtgggtcaag cagcagtttg gccggattgc agcgcaagcc cgatgagggc
     3241 tcccacgggc cgtgcaccct gagcccgtaa cgctgacctg caccgatggc gggcagcatg
     3301 ccgtggaaga cgtgcgcggt gcgctggggc aactcgacga cggtctgcga cccgtcctcg
     3361 gcgaacaggc agacctcgat cgcgtcggca atctcggacg caacggcgaa gttggtgcca
     3421 ccctcggtcg tcgtcgcacc aagggggaag gggagtccag caagaggaag agtcacggac
     3481 ccccgatgcc cccgactgag gttgctaaac gcggacgtcg tttggccggc gcacaggtgg
     3541 ggagagcccc ggctgctgct cgtttcatga tggagacccg cgatgccccc acgctcgacc
     3601 tatcggctgc agatcacccg agagtttcgg ttgcaatccg cggccaccct cgtcgactac
     3661 ctgtcggagc ttggaggcgc ggccgtgtac gtatcgcagc tgctcgcggc gacgcgcgga
     3721 tcgcag
//
LOCUS       DASWVF010000369         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_188250, whole genome shotgun sequence.
ACCESSION   DASWVF010000369 DASWVF010000000
VERSION     DASWVF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188250"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..314
                     /locus_tag="VGD55_06630"
     CDS             135..314
                     /locus_tag="VGD55_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870054.1"
                     /translation="MTRSKPVRWFDSLFVFVGMGWVLLWQLVTPEYRVRVPVRARSSR
                     LLKKQAQGIDTRSGV"
     gene            327..1427
                     /locus_tag="VGD55_06635"
     CDS             327..1427
                     /locus_tag="VGD55_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0870055.1"
                     /translation="MRGEQDSQVELLTTTSPGKRVPATHPIREIKRLADLALAKLDST
                     FDAMYSSIGRPSIPPERLLKAQLLIAFYSVRSERQFCERLDYDLLFRFFLDMNLDDAS
                     WDATTFTKNRDRLVEHEVARQFFEEVVRHAKKAGLVSAEHFTVDGTLIEAWASMKSFR
                     KKGEKPEDRPPPDDRGNPTVNFHGEKRSNKTHESTTDPEAQLARKGKGKESKLSYSGH
                     VLMENRNGLCVDVRVAPATGYAERDEALEMLRRLRRRGYDPKTLAGDKGYCLGDFPQR
                     VVDLGIKPHLAVPDNAPTRSPARRFVRSEGYKVSQVIRKRVEEIFGWGKSVAGLRRTK
                     MKGRRRTWDGSYLIMAAYNLTRMTRLLRAAAA"
     gene            complement(1786..2969)
                     /locus_tag="VGD55_06640"
     CDS             complement(join(1786..2705,2705..2969))
                     /locus_tag="VGD55_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0870056.1"
                     /translation="MEEQRKRRARRKFTDEFKARAVRLVLKEGQTAAQVARDLDLVES
                     VLHGWVRQAKIDRGMGPAGALTSEEREELSRLRKENRVLKEERENLKKSGGLLRQGRP
                     MRFAFIGAEKARHSVPMLCRMLKVSRSGYYAWRGRGPSRRAVEDARLGTLIVASHRDG
                     RGTYGSPRVLDDLREWGERVSRKRVARLMKERNLVGEMKKRWRHPASAPSDAVVAPNT
                     LGRAFNVPEPNRVWASDITYVRTHEGWLYLAVVIDLFSRRVVGWSIQPSMHTDIVLSA
                     LTMAVGQRLPGPGLLQHSDRGSQYTSDDYQRALRAHGIECSFSGRGNCWDNAVVESFF
                     GTLKRELIHRRSWPTRREAADAIHEYIEVFYNRRRRHTTLGGKAPAVFEALAERQVAQ
                     AA"
     gene            2968..>3304
                     /locus_tag="VGD55_06645"
     CDS             2968..>3304
                     /locus_tag="VGD55_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0870057.1"
                     /translation="MDTIYSFRVVSTKAGQDQFLTGRIDETTSFDQTDFRSTIPRLQR
                     EAREANRVFVDLLAAIGARMNATPAQMALAWLLARKPWIVPIPGTTKVDRLEENLGAA
                     SLPLSREDMQ"
BASE COUNT          577 a         1077 c         1065 g          585 t
ORIGIN      
        1 tcatgtggcg cgaaaccctc cttgcgaatg acgactccta atcgtatagc ttggaccact
       61 gagcccgttg cggagtgggc tgcagatgat tcggccagcc cagcaacctg gtggaccccg
      121 ctaaggagga caccatgaca cgctctaagc cagttcgctg gttcgattcg cttttcgtgt
      181 tcgttggcat gggttgggta ctgctttggc aattggtgac cccggaatac cgggtccgcg
      241 tgccggtgcg cgcgagatct agcaggctgc tgaaaaagca ggcccagggg atcgacacca
      301 gatccggggt gtgatctgct gtgggcatgc gcggagagca ggattcgcag gtggagcttc
      361 tgacgacgac gtcgccgggc aaacgggtcc cggcgacaca tccgattcgc gagatcaaac
      421 gtctcgccga tctcgcacta gccaagctcg actcgacgtt cgacgcgatg tactcgtcga
      481 tcggccgccc atcgatcccg ccggagcggt tgttgaaggc tcagttgctg atcgcgtttt
      541 actcggtccg ctccgagcgc cagttctgtg agcgcctgga ctacgacctg ctgttccggt
      601 tcttcctcga catgaatctc gacgacgcga gctgggacgc gacgacgttc acgaagaatc
      661 gcgatcgact cgtggagcac gaggtcgcgc gccagttctt cgaggaagtc gtgcgccacg
      721 cgaagaaggc cgggctcgtt tcggcagagc acttcaccgt cgacggcacg ctgatcgagg
      781 cgtgggcgag tatgaagagc ttccgcaaga agggcgagaa gcctgaggat cgcccgccgc
      841 ccgacgaccg cggcaatccg acggtgaact ttcacggcga gaagcgatcg aacaaaacgc
      901 acgaatcgac gacggatccc gaggctcagc tggcgcggaa gggcaaaggc aaagaatcga
      961 agctgagcta ttcggggcac gtgttgatgg agaaccgcaa cggcttgtgc gttgatgtcc
     1021 gcgtcgctcc agcgacgggc tacgccgagc gagatgaggc tctggagatg cttcgacggc
     1081 ttcgccgtcg tggctacgat ccgaaaacgc tggcgggcga caagggctac tgcctcggcg
     1141 atttcccgca gagagtggtc gaccttggca tcaagccgca cctggcggtg ccggacaacg
     1201 caccaactcg gtcgccagca cggcgcttcg tccgcagcga gggctacaaa gtcagccagg
     1261 tcattcgcaa acgggtcgaa gagatctttg gctggggcaa gtccgtcgct gggctgcgcc
     1321 gaacgaagat gaagggccgc cgaaggacgt gggacggcag ctacctgatc atggccgcgt
     1381 acaacctcac gcggatgaca cggctgcttc gcgccgcggc tgcgtgaacc ggaggggctc
     1441 ggggcacctc gtgccccggg ccgcctcgtt attcaccacg acgcggcccc tgatcccgag
     1501 aaatccggcg cagaagccgc cttcttcagc agcctgctag tggaccagtt cggacagacc
     1561 ggcggagtcg gcttctcggt atgaaacgcc gccaagcagc ggtcacgacc agaggcgccg
     1621 ccgccgcatt acgtggcgtc tgtcggtgaa aaaacaaccg cgagtccgcc cttgtccggc
     1681 ccggacgtgg cgctggcgat cacgacgttg ttcgggtcga tttcctgggt gccgcaggcg
     1741 gcaaggccca gcagtgatcc tgccccgatt cagtggactg tttcgttacg ctgcctgagc
     1801 gacctgcctt tccgcaaggg cctcgaatac ggcaggcgcc ttgccgccga gggtcgtgtg
     1861 gcgacggcgg cggttgtaga agacctcgat gtactcgtgg atggcgtcgg cggcctctcg
     1921 gcgcgtcggc cagctgcgcc ggtgaatgag ctcgcgcttc agcgtcccga agaagctctc
     1981 gacgacggca ttgtcccagc agttgccgcg gccgctgaag ctgcactcga taccgtgggc
     2041 gcgtagcgcc cgttggtaat cgtcgctcgt gtactgcgag ccgcggtcgg agtgctgcag
     2101 cagccccggt cctggcaggc gctggccgac ggccatcgtg agagccgaga gcacgatgtc
     2161 cgtgtgcatg ctcggttgga tcgaccagcc gacgacccga cgggagaaga ggtcgatgac
     2221 gacggccagg tagagccagc cctcgtgcgt gcgcacgtac gtgatgtcgc tcgcccacac
     2281 gcggttcggc tcgggcacgt tgaaggcccg gcccagggtg ttcggtgcca cgaccgcgtc
     2341 gctgggcgcc gaagctgggt ggcgccatcg cttcttcatt tctccgacga gattccgttc
     2401 cttcatcagc ctggccaccc gtttgcggct tacccgctct ccccactcgc ggaggtcgtc
     2461 gagcacgcgc gggctgccgt acgtgccgcg cccgtcccgg tgtgaggcca cgatgagcgt
     2521 ccccagccga gcgtcctcca cggctcggcg cgacggtcct cgcccgcgcc aggcgtagta
     2581 cccgctccgc gagaccttca gcatgcggca gagcatcgga accgagtgcc gagccttctc
     2641 cgctccgatg aacgcgaatc tcatcggccg tccttggcga agaaggccgc cgcttttttt
     2701 aagatctccc gctcctcctt caggactcgg ttctctttgc gtagccgcga cagctcctcg
     2761 cgctcctccg atgtcagcgc acccgctggc cccatcccgc ggtcgatctt cgcctgtcgt
     2821 acccagccgt gaagaaccga ttccaccagg tccagatcgc gcgcgacctg cgcggccgtc
     2881 tggccctcct tcagcaccag gcggacggcc ctggccttga actcgtcagt gaacttgcgg
     2941 cgagcacgcc gcttcctctg ctcctccatg gacaccatct actccttccg ggtggtgtcc
     3001 accaaagcgg ggcaggatca gttcctcacc gggcgcattg acgagacgac gagcttcgat
     3061 cagaccgatt ttcgatcgac catcccgcgc ttacagcgcg aagcccgcga agccaaccga
     3121 gtgttcgtgg acctgctcgc cgctatcggc gcccgaatga acgcgacccc agcgcagatg
     3181 gccctcgcct ggttgctcgc gcgcaaaccc tggatcgtgc cgatcccggg gacgaccaag
     3241 gtcgaccgcc tcgaggagaa cctcggcgcg gcgagcctgc cgttgagccg cgaggatatg
     3301 cagg
//
LOCUS       DASWVF010000370         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_9972, whole genome shotgun sequence.
ACCESSION   DASWVF010000370 DASWVF010000000
VERSION     DASWVF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9972"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VGD55_06650"
     CDS             complement(<1..1326)
                     /locus_tag="VGD55_06650"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0870058.1"
                     /translation="MSIGANVVAADGFVPSVATASHVTKRFGATVALKDVGIDVQAGD
                     VHALVGRNGAGKSTLVSILTGLQTPDVGELRFSGKAPPARSDREAWGRVVACVYQHLT
                     IIPTLTVAENLFLNRQTAATPVISWSKLRHRASELLSSWEMDVDVRRLAGDLTVEQRQ
                     LVEIARALSFGARFIILDEPTARLDAAGVARLLGRIRTLQQQGVTFLFISHHLQEIFE
                     LCNTVTVFRDARKVLTAPVSGISHGELVDAMTGEHVSVGDDPRPPRPTTGEAAAKPLL
                     LVTDLTVTPGCHNVTFEVLPGEIVGVAGGGGSGKIAVAEAIVGLRRPDCGEVRVEGVV
                     LRAGNVTAALKAGVGFVPEDRHDQGFVAEMSIGENVTMPVWDRLKSYGVVLSPRRRSA
                     RAQSIIKDLAIVTPGPELVTSALSGGNQQKVVMGRALVSTPRVLVLMAPT"
     gene            complement(1372..>2512)
                     /locus_tag="VGD55_06655"
     CDS             complement(1372..>2512)
                     /locus_tag="VGD55_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY0870059.1"
                     /translation="RISAVVIASVLLAAGCSSKKSSTATTPTSAAPSTAAATSAAPAT
                     TSAATPATSAAAAPTSAAAGGGLIGVDYPRSDSDFWNAYIRYVPQMATTLGATIKTSA
                     SGNDITKLNANADSLVAEGAKALVLAPQDTAGIIPELSKLAGENIPVVTIDTEPDSGK
                     VFMIVRADNKAYGTKSCNYIGTALKGVGNVVEFEGDLASVNGRDRSTAFGACMAANFP
                     KIKVFAEPTKWDTPTAIGQLNTVLAGNKIQGLYMQASIYLSATLADLQSKGLLKPAGS
                     AGHIVMVSNDGVKAEFTAIQAGQLDATIDQPADLYAKYGLYYAQQALAGKTFALGPTD
                     HNSNIVTAADGDLEDQLPASLVTVDGAYPGSVKSTDPSLWGNQSS"
BASE COUNT          449 a          817 c          793 g          453 t
ORIGIN      
        1 ggtcggagcc atcaacacca agacgcgcgg cgtgctcacc agcgcgcggc ccatgaccac
       61 cttttgctgg ttgccacccg aaagcgccga cgttacgagc tcgggacccg gagtcacgat
      121 agcgaggtcc ttgatgatcg actgcgctcg cgctgatcgc cggcgcgggc tcagtacgac
      181 gccgtagctc ttgagccgat cccagaccgg catcgtcacg ttctcgccga tgctcatctc
      241 ggcgacgaaa ccttggtcgt ggcgatcctc gggtacgaac cccacgccgg ccttcagcgc
      301 tgccgtgaca ttgcccgccc gaagcaccac gccttcgacg cgcacctcac cacagtcagg
      361 gcgccgcagc ccaacgatgg cttccgcaac ggcgatcttt ccgctgccgc cgccgccggc
      421 aacaccgacg atctcgccgg gcaacacctc gaacgtcacg ttgtgacaac cgggggtcac
      481 cgtgaggtca gtgacgagca gcagcggctt agcagcagcc tcaccggtgg tgggtctcgg
      541 cggccgcgga tcgtcgccga ccgagacgtg ctcacccgtc atcgcgtcga cgagttcacc
      601 gtgcgagatc cccgacacgg gcgccgtcag aaccttccgt gcatcacgaa acaccgtgac
      661 ggtgttgcac aactcgaaga tctcctggag gtggtggctg atgaacaaga acgtgacgcc
      721 ctgctgctgc aaggtgcgga tgcgcccgag caaacgtgcg acgccagcag cgtcaaggcg
      781 agcggtgggc tcgtcgagga tgatgaaccg tgcgccgaac gacaaagcgc gggcaatctc
      841 gacgagctgg cgttgctcga cagtgaggtc gccggcgagc ctgcgcacat cgacgtccat
      901 ctcccaggag ctgagcaact cagacgcgcg gtgacgaagc ttcgaccagc tgatcaccgg
      961 tgtcgcagcc gtctgccgat taaggaacag gttttcggcg acggtgagcg tcgggatgat
     1021 tgtcaggtgc tggtagacgc aagcgacgac ccgcccccaa gcttcacggt cggatctcgc
     1081 tggcggcgcc ttaccgctga acctcagctc gccgacatcc ggcgtctgta atccggtaag
     1141 gatcgagaca agcgtcgact tgcctgcacc gttgcggccg acgagcgcat ggacatcgcc
     1201 tgcctgcacg tcgatgccga cgtctttaag ggcgacggtc gcgccgaacc tttttgtcac
     1261 atgcgacgca gtggcgacgg atgggacgaa cccatccgcc gccacgacgt tggcgccgat
     1321 cgacacgagc ttctctttcc tagatctctg ttactgccga gcgaggttcg attagctcga
     1381 ctggttaccc cacaaggacg ggtctgtcga cttcaccgac ccggggtagg cgccgtcaac
     1441 tgtcaccagc gaggcgggca actggtcttc gaggtcgccg tccgcggcag taacgatatt
     1501 gctgttgtga tcggttggcc ccaacgcgaa cgtcttgccc gcaagcgcct gctgtgcgta
     1561 gtacaggccg tacttcgcgt acaggtcggc cggctggtcg atcgtcgcgt cgagctgtcc
     1621 tgcctggatt gccgtgaact cagccttcac gccgtcgttg gagaccatca cgatgtgccc
     1681 ggccgaacct gccggcttca gcagaccctt gctctggagg tcagcaagcg tcgccgacaa
     1741 gtagatgctc gcctgcatgt agagcccctg gatcttgttg cccgccagca ccgtgttcag
     1801 ctgaccgatc gcggtcgggg tgtcccactt ggtcggctcg gcgaagacct tgatcttcgg
     1861 gaagttcgcc gccatgcatg caccgaacgc ggtgctgcgg tcgcgaccgt tgaccgacgc
     1921 gagatcgccc tcgaactcaa ccacgttgcc gacgcccttg agagcggtac cgatgtagtt
     1981 gcacgacttc gtgccgtacg ccttgttgtc ggcgcgcacg atcataaaga ccttgccgct
     2041 gtcaggctcc gtgtcgatcg tgacaaccgg aatgttctcg ccggccagct tcgacagctc
     2101 agggatgatg cctgcggtgt cttgcggggc gagaacaagt gccttcgcgc cctcggccac
     2161 caacgaatcg gcgttcgcgt tgagcttggt gatgtcgttg cccgaagcac ttgtcttgat
     2221 cgtcgcgccc agcgtggttg ccatctgcgg cacgtacctg atgtaggcat tccagaagtc
     2281 ggagtcggaa cgcggatagt cgacgccgat caggccgccg ccggcggcgg ccgacgtcgg
     2341 agcggcggca gcagaggtcg ccggagtggc agcggacgtg gtcgcaggag cagccgatgt
     2401 agccgcagcc gttgaagggg cggcactcgt tggggtagtg gccgtactgg acttcttcga
     2461 actgcatcct gccgcaagca gaaccgacgc gatcacaacc gcgctgatcc gc
//
LOCUS       DASWVF010000371         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_137468, whole genome shotgun sequence.
ACCESSION   DASWVF010000371 DASWVF010000000
VERSION     DASWVF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137468"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..785
                     /locus_tag="VGD55_06660"
     CDS             114..785
                     /locus_tag="VGD55_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870060.1"
                     /translation="MVGNARRHLMPTAGAAALVLSMAFVLAGCSKSHHSAALIPKTSV
                     SAANSAAGGGGAGAGASSAAAGGGAAQSAAGGGNTELAIGPVCKWLTAAQVTAASGKP
                     LTDLTGDISGGPQGVLTYHSCTWTPDGGTGSGINIEVGTWPSGSAQLATQKANDESQA
                     SSIPGAATTTTVSVGDGGYDVNYVSAKTDTVTFSKGNSVVSVEVDNGVPGAALSLANL
                     IEPKV"
     gene            complement(792..2090)
                     /locus_tag="VGD55_06665"
     CDS             complement(792..2090)
                     /locus_tag="VGD55_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0870061.1"
                     /translation="MPVVQSVDVVDVRFPTSLDRDGSDAMNQDGDYSAAYVVLSTDEP
                     ELAGFGLTFTIGRGNELCVAAAREQAALLVGMDVDVMAGDMGAVYRILTADSQLRWLG
                     PEKGVVHLSLAAVLNAAWDLAARRAGVPLWRLLVGMSANQLAAVADLRYVSDVLTRAE
                     AESMLAALEPSRAERIAVLEQDGYPCYTTSPGWLGYDDEKLARLCHQAVAAGFSYIKL
                     KVGANLDDDRRRCRIARSILGPDRHLMIDANQVWDVGQAIEWVKALAEFEPLWIEEPT
                     SPDDILGHATIRAAIAPIGVATGEHAHNRVMFKQFLQAGAINYAQIDSCRLASVNEII
                     TVLLMATKFGVPVCPHAGGVGLCELVQHLSIFDYVAVSGSLDGRVTEYVDHLHEHFTD
                     PCIVKNAAYVLPTAPGYSARMHDASVSTYRYPDGDYWTQA"
     gene            2355..2984
                     /locus_tag="VGD55_06670"
     CDS             2355..2984
                     /locus_tag="VGD55_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0870062.1"
                     /translation="MRYRPLGSTGLQVSVLAFGASSLGGVFRSIDEGSGIAAVHTALD
                     LGINLIDVSPYYGLTVAETVLGRALKGVDRDSYLLATKVGRYGEAEFDFSPERVVASA
                     HESMRRLGVDYLDVLQCHDIEFGDLDQIVDETLPALEGLRAAGLVRFIGVTSFPVPAL
                     VAVARRHRIDTVMSYCNYTIENRNLEPWLADLEGEGLGVMNASRWGWVC"
     gene            2996..3310
                     /locus_tag="VGD55_06675"
     CDS             2996..3310
                     /locus_tag="VGD55_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870063.1"
                     /translation="MPGWHPAPASVVAACSDAASLCAARGSDISKLAIQFAVQGPVAT
                     TVVGSADAANMARNVAWVDEEIDFDLLRDVESLLAPVRDIGWSIGRPENADPPATPRA
                     RR"
     gene            3307..4164
                     /locus_tag="VGD55_06680"
     CDS             3307..4164
                     /locus_tag="VGD55_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0870064.1"
                     /translation="MSEPHRIIDSHIHLWDPSVLRYPWLDSEPDLRRPFLPADLRGSC
                     GVTEWVVVQADAQDARDQGIAELEWVASLAASEPSLRGMVVRAPVESGRAVLDLLELV
                     AAHPLTVGVRRLIQDEPVGFAQSARFIDGVRAVGEFGLPFDVCVRAAERGDIAPLVDA
                     CPDVSFVLDHLGKPDIAGGEWGPWREQLIDLARRPNVVCKLSGSTTEAGAGWREYAVR
                     PYLEQALESFGANRCMFASDWPVASLTTTYPRWLDLVSEVVAGCSDAEIDDVFAGTAR
                     RVYAMTKGS"
     gene            4167..4490
                     /locus_tag="VGD55_06685"
     CDS             4167..4490
                     /locus_tag="VGD55_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY0870065.1"
                     /translation="MQRFGSVVRLRPEHRDDYLALHANVWPDVEAALTRANVRNYTIF
                     LHGDLLFGYYEYVGADHAADLARIAADPQTQRWWALTDPCQERLAGTPDGSQWAPMAH
                     IWHLE"
BASE COUNT          786 a         1535 c         1539 g          829 t
ORIGIN      
        1 tcgcgccgct gcttcaccac ccgccgtccc aactcgggta cgtaattcat tactgatgcc
       61 tagcccctgc cacgcttcta gcgttcggtg caggctcatg tgcgggagga atcatggtgg
      121 gtaacgcccg gcggcacctc atgcctacgg ctggtgccgc agcgctcgta ctgtccatgg
      181 cattcgtgct tgccggctgc tcgaagtccc atcacagcgc ggcgttgata ccgaaaacat
      241 cagtgagcgc cgccaattcc gcggcgggcg gtgggggcgc gggtgcgggc gcctccagcg
      301 ccgccgcagg cggtggtgct gcgcagtcgg ccgctggcgg cggcaacaca gaactcgcta
      361 tcggtccggt gtgcaaatgg ctcacggctg cgcaggtcac cgccgcatcg ggcaagccct
      421 tgaccgacct gactggcgac atcagcggcg gaccgcaagg ggtactgacc taccactcct
      481 gcacctggac tcccgacggt ggtaccggta gcggcatcaa catcgaggtc ggcacctggc
      541 catcggggtc ggcgcagctc gccactcaga aggccaacga cgagtcccag gcgagctcaa
      601 ttcctggcgc cgcaacgacg acaacggtga gcgtcggtga cggcgggtac gacgtcaact
      661 atgtatcggc caagaccgac accgtcacgt tctccaaagg caacagcgtc gtcagcgtcg
      721 aggtcgacaa cggcgtgccg ggagcggcgc tctcgttggc gaacctcatc gagcccaagg
      781 tctaggccga tttaggcctg cgtccagtag tcgccgtcgg ggtagcggta ggtgctgacc
      841 gacgcgtcgt gcatgcgggc gctgtagccg ggcgcggtcg gcaacacgta ggccgcgttc
      901 ttcacgatgc acgggtcggt gaagtgttcg tgtaggtggt cgacgtactc ggtgacccgg
      961 ccgtcgaggc tgcccgacac cgcgacgtaa tcgaagatcg acagatgctg aaccagttcg
     1021 cacaacccga ccccgccggc gtgcggacat accggcactc cgaacttggt ggccatcaag
     1081 agcacggtga tgatctcgtt cacactggcg agccggcatg aatcgatttg ggcgtagttg
     1141 atcgcgccgg cctggaggaa ctgcttgaac atcacccggt tgtgggcgtg ctcaccggtg
     1201 gcgaccccga tcggcgcgat cgcggctcgg atcgtcgcat ggcccaagat gtcgtcgggg
     1261 ctcgtgggtt cttcgatcca cagcggctcg aattcggcga gcgccttgac ccactcgatt
     1321 gcctggccga cgtcccatac ttggttcgcg tcgatcatca gatggcggtc gggacccagg
     1381 atcgatctgg cgatacggca tcggcggcgg tcgtcgtcga gattcgcgcc caccttcaac
     1441 tttatgtagg agaatcccgc ggccaccgcc tggtggcaca gccgcgccaa cttctcgtcg
     1501 tcgtagccca gccagcccgg tgatgtcgtg taacacgggt agccgtcctg ctcaaggacc
     1561 gcgatgcgtt ccgcgcgcga cggctcgagc gcggccaaca tcgactccgc ctccgcgcgc
     1621 gtcagtacgt cggagacgta ccgcagatcg gccacggccg cgagctggtt cgcggacatg
     1681 ccgaccagca agcgccataa cgggacgccg gcccggcgcg ccgcgaggtc ccacgccgca
     1741 ttcaagacgg cggccaacga caggtgcacg acgccctttt ccggacctag ccaccgcagt
     1801 tgggagtctg cggtcaggat ccggtagacc gcacccatgt cgccagccat gacgtcgacg
     1861 tccatgccga cgagcaacgc cgcctgctcg cgcgccgccg caacgcacag ttcgttcccg
     1921 cggccgatcg tgaacgtcag tccgaagcca gccagctccg gctcgtcggt cgacagcacg
     1981 acgtaggccg cggagtagtc gccgtcctgg ttcatggcat cggaaccgtc gcggtcgagc
     2041 gacgtcggga accgcacgtc gaccacatcg acggactgaa caacaggcac gagcaacccc
     2101 agaagtgatc ggatgtaacg cgtaacggta gggtcagggt ccgcaaaggt caaacgattc
     2161 gggagcccag gtgcgagccc attcagttta ctgcgctgca ccggcccaac tcatcggcct
     2221 gtttgcgcgg ccaggtctgt cattgatcgg atgacttcgc tagggttttg ggcagtcgcg
     2281 aacgggccat caccagcgcc gtccgctaag tgcactgccc ggcccacccg tcccgaccgg
     2341 cccgaggagc agcgatgcga taccggccgc tgggatcgac ggggctccag gtctccgtcc
     2401 tcgcttttgg agcctcatcg ctgggtgggg tcttccgttc gatcgacgag gggtccggga
     2461 tcgcggccgt gcacacggcc ctcgacctag gcatcaacct gattgacgtg tccccgtact
     2521 acgggttgac cgttgccgag accgttctcg gtcgtgcgct gaagggggtc gatcgcgact
     2581 cgtacctgct cgcgacgaag gtgggtcgat acggagaggc cgagttcgat ttctcgcccg
     2641 agcgggtggt ggcgagcgcg cacgagagca tgcgccgcct cggcgtcgac tatctcgatg
     2701 tgctgcagtg ccacgacata gagttcggcg atctcgacca gatcgtcgat gagacgctcc
     2761 cggcactcga aggcttgcgc gccgccggac tcgtaaggtt catcggcgtg acctcttttc
     2821 cagtgcccgc gctggtggcc gtcgcgcgcc gtcatcgaat cgacaccgtg atgtcctact
     2881 gcaactacac gatcgagaac cgcaatctcg agccgtggct ggccgacctt gagggagagg
     2941 gacttggcgt catgaacgcc tcccgctggg gatgggtctg ctgacgaacc gcggcgtgcc
     3001 gggatggcac ccggcgccgg catcggtcgt ggccgcgtgt tccgacgcag cctctctatg
     3061 tgccgcccgc ggttctgaca tttccaagct cgcgattcag ttcgccgtgc aggggccggt
     3121 agcgacgacg gtggtcggca gtgccgatgc tgcgaacatg gctcgcaacg ttgcgtgggt
     3181 cgacgaggag atcgactttg atctgctgcg cgatgttgag tcgcttctcg cgccggtgcg
     3241 cgacatcggc tggtcgatcg gtcgccccga gaacgccgac cctcccgcaa ctccgcgcgc
     3301 gcgacgatga gcgaacccca ccgcatcatc gactcgcaca tccatctttg ggatccgagc
     3361 gtcctgaggt acccctggct cgactccgag cccgaccttc gccggccgtt cctgcccgct
     3421 gatctgcgtg gcagttgcgg tgttaccgag tgggtggtcg tgcaggcgga cgcgcaggat
     3481 gctcgtgatc aaggcatcgc cgagctggaa tgggtcgcca gcctcgcggc gtccgaaccc
     3541 tcgcttcgag gaatggtcgt ccgcgcaccc gtcgagagcg gtcgggctgt cttggatctg
     3601 ctcgagctgg tcgcggcgca tccgctgaca gtcggcgtcc ggcggctgat ccaggatgag
     3661 ccggtcggct tcgcgcaaag cgcgaggttc atcgacggcg tccgcgcggt tggcgagttc
     3721 ggtttgccgt tcgatgtctg cgtccgcgct gcggaaagag gcgacatcgc tcccctcgtt
     3781 gacgcgtgcc ccgacgtcag cttcgtgctc gatcatctcg gcaaacccga catcgccggc
     3841 ggtgaatggg ggccgtggcg cgagcagctg atcgatcttg cgcgacgtcc caacgtggtc
     3901 tgcaagctct cgggttcgac cactgaagcc ggagccggtt ggcgcgagta cgccgtccgg
     3961 ccttacctcg aacaggcgct cgaatccttc ggggcgaacc ggtgcatgtt cgccagtgat
     4021 tggccggtcg ccagcctcac gacgacctac ccgcgatggc tcgacctagt gtctgaagtg
     4081 gtcgccggtt gttccgacgc cgaaatcgac gacgtgttcg cgggcacggc gcggcgcgtc
     4141 tatgcaatga cgaaaggcag ctgatcatgc aacgcttcgg cagcgtggtt cggcttcggc
     4201 cagaacaccg cgacgattac ctggccttgc acgcgaacgt ctggcccgac gtcgaggccg
     4261 cgttgactcg cgcgaatgtc cgcaactaca cgatcttcct ccacggtgat ctgctcttcg
     4321 ggtactacga atatgtcggc gctgaccacg cggccgacct cgcccgcatc gcggccgatc
     4381 cgcagacgca acggtggtgg gcgctcacag atccgtgcca agagcggttg gcagggacgc
     4441 cggacggttc gcagtgggct ccgatggcgc acatctggca tctcgaatga ctgactcggc
     4501 agcaccagga aggcaacaat gcgcgcactg aggtacgtag ccgccggcaa cgtcgaggta
     4561 caggagatcg acccgacgcc acctggcgag ggccaggtgc agatcgaggt cgcgttcacc
     4621 ggaatctgcg gcaccgacct gcacatcttc cacgggtcta tggatgcccg agtcgctcgt
     4681 ccggctgtg
//
LOCUS       DASWVF010000372         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_443204, whole genome shotgun sequence.
ACCESSION   DASWVF010000372 DASWVF010000000
VERSION     DASWVF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443204"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1659)
                     /locus_tag="VGD55_06690"
     CDS             complement(22..1659)
                     /locus_tag="VGD55_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0870066.1"
                     /translation="MSLSRAASDGRARDGVPVLAIAVRISDYLIPAVATAATLIILGL
                     VGALHSVSWLAAVFAVVVVSASCGAASGVVRGRCNASMDVVISDLHRIVAGDLRVRTD
                     VTSQGQLRDLEIAVRDIVGQMRVLVSAADRCADLLSGGWQTMNEVAWSMMNASEGTVV
                     EVTAAARAAAEVSQSIQFIASATEETTATMREVAAHAIDASGLGQSGVEQITVAGRSV
                     EDLRDASKRVEEVLRLINGVARQTHLLALNATIEAARAGEHGRGFAVVASEVKLLAEQ
                     TASATGNVAATMREIDEGSEHAGSSMLSVSETIYGMSTRQHSIAAAVEQQTSTTQAIA
                     RSTADAADQSVALASSVRSLTNAVRLGAYAGAKARTVAAEVATIEQTLHSIVERYTFD
                     HVEQEAADVDTGPVRGVSTVNGVTVVPNEAMGTAINQCSFEGTWGHAAANLESDGSNA
                     HSSMPGDTCTVRFVGTRIRFYGVVAANHGKASITVDGLEPAVIDQYAPEREQGGLGWQ
                     SPVLPRGEHVCVLTVLAESNPSSRYVWVNVDKFEVEA"
BASE COUNT          315 a          612 c          576 g          322 t
ORIGIN      
        1 acccgcgggt cgggtcgagg atcaggcttc gacctcgaac ttgtcgacgt tcacccagac
       61 atagcgtgag ctcgggttcg attcggccag cacggtaagt acgcagacgt gctctcctcg
      121 cggcaggacc gggctttgcc acccgaggcc gccctgctcc cgctccggcg cgtactggtc
      181 gatgacggcg ggctcgagcc cgtcgacggt gatggaggct ttgccatggt tcgccgcgac
      241 aactccgtag aacctgatgc gggtaccgac gaatcgaacc gtgcaggtgt cgccgggcat
      301 gctcgagtgt gcgttcgaac cgtctgactc caagttcgcg gccgcgtgtc cccacgtgcc
      361 ttcgaagctg cactggttga tcgcggtgcc catcgcttcg ttagggacga ccgtcacccc
      421 gttcacagtg ctcacgcctc gcaccgggcc agtgtcgacg tccgcggctt cctgctcgac
      481 atgatcgaag gtgtaccgct cgacgatcga atgcagcgtt tgttcgatcg tcgcgacttc
      541 ggccgcaacg gtgcgcgcct tggcgcccgc gtacgcgccg agccggacgg cgttcgtgag
      601 cgacctgacg ctcgacgcaa gtgcaacaga ctgatccgca gcgtctgcag tactgcgcgc
      661 gatggcttgc gtcgtagacg tctgctgctc gacagcggca gcgatggagt gttgccgcgt
      721 gctcataccg tagatggttt cgctgacgct gagcatcgac gagccagcgt gctcggatcc
      781 ctcgtcgatc tcccgcatcg tcgcggcgac attgccggta gcagaggcgg tctgctcggc
      841 gaggagcttg acctcacttg ccacgaccgc aaacccgcgg ccatgctcac cggcgcgcgc
      901 tgcctcgatc gtcgcgttca acgcaaggag gtgcgtctgc cgcgccactc cgttgatgag
      961 tcgaaggact tcctcgactc gcttcgacgc atcccgcaga tcttcaacac ttctgccggc
     1021 gacggtgatt tgctccacgc cggattggcc aaggcccgat gcatcgattg cgtgtgcggc
     1081 gacctcgcgc atcgtcgccg tcgtctcctc ggtggcgctc gcgatgaact ggatgctctg
     1141 cgagacctcg gcggccgcac gtgccgcagc ggtgacctcc acgacggtgc cctccgacgc
     1201 gttcatcatc gaccaggcaa cctcgttcat ggtctgccat ccgcccgaga gcaagtccgc
     1261 gcaacggtcg gcggcagaca ccagcacccg catctggcca acgatgtcgc gcaccgcgat
     1321 ttcgaggtcg cgcagttgtc cttgcgaggt cacgtcagtg cgcactcgaa gatcacctgc
     1381 gacgatccga tgcagatcac tgatcacgac gtccatcgag gcattgcagc ggccacggac
     1441 cacgccggac gccgcgccgc acgaagcgct gaccaccacg acggcgaaga cggcagcgag
     1501 ccacgaaacc gagtggagag cgccgacgag gcccaagatg atcaacgtcg ccgcggtcgc
     1561 gaccgccggg atcaaatagt cgctaattcg gacggcgatg gccaaaacgg gcacgccgtc
     1621 gcgtgctcgg ccatcggacg cggcgcgtga aagcgacatc gatggctcct ggtcgaaggg
     1681 gaaactcccc cgttattcga ccggaatcgc ctcgggttga ggccggcagc cggggcagta
     1741 gccctaatgt gaggcgctat gagtgacgac ctcgcgccga ttggaccaga cgaacgctcg
     1801 cgtgcatacg ccaccttgat cttgg
//
LOCUS       DASWVF010000373         9282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_315391, whole genome shotgun sequence.
ACCESSION   DASWVF010000373 DASWVF010000000
VERSION     DASWVF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315391"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2876
                     /locus_tag="VGD55_06695"
     CDS             <1..2876
                     /locus_tag="VGD55_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0870067.1"
                     /translation="QAVVLRAYAKYLRQAGYRFSQAYLESALRANTHVVRLLVSLFET
                     RLDPAFPGDRASGETAIAHELDGALDAVTSLDEDRILRSIATLIRATVRTNYWQRSAE
                     GAPKGYLSLKLDSQAVPDLPEPRPWVEIFVYSPQMEGIHLRFGPVARGGIRYSDRRED
                     FRTEVLGLAKTQTVKNAVIVPVGSKGGFVVKRPPADVSDRRAMQAEVVACYRTLICGL
                     LDLTDNLIADSEGRRTVPPKDTVRHDGDDSYLVVAADKGTATFSDIANGIAAEYGFWL
                     GDAFASGGSAGYDHKAMGITARGAWESVTRHFRELGVNCQTTDFTCVGIGDMSGDVFG
                     NGMLLSTHIRLLAAFDHRHIFIDPSPDAAASFAERRRLFALAGSSWADYDLALISAGG
                     GVWPRSAKSVPISAEMAAALGVKAAAMAPADLMRAILRAPADLLWNGGIGTYVKAHTE
                     SHADVGDRANDAIRVDGRELRCRVVGEGGNLGFTQLGRLEFALGGGHINTDAIDNSAG
                     VDTSDHEVNIKILLDGAARAHELDLAERGPLLVSMTDEVGALVLRDNYAQNLALSCSQ
                     TQAPTMLHVHAAYIEWLEKNGHLVRALEHLPDAEAISARRAAGLGLTRPELALLLAYA
                     KITATRDIVGSDLPDDPFTRRALVDYFPSPLRERFATAMTEHPLRREIIATQLANELV
                     NLSGTSFLYRVVSETAASTSDITRAHSAARMSFRVDQIWSDIEGLDDVVASDVQTELL
                     LGVRQLLDRATRWFLANRRPPLDVPETVDQWQPRIAAVLDALPQRLRGRDAENYGAAL
                     GRYVGAGVPDSIALGAVALTTGLSALAIVDIALQTKRDVDEVAGVHFAIADVLGLSRL
                     GSLIAALPRDSRWHTLARAAARDDLEAAHAELTTDVLLSTGPTATADDRISAWQQANG
                     AALARATSFIADISVSDGADLATLSVALREVRALARAAALPHA"
     gene            complement(2880..4028)
                     /locus_tag="VGD55_06700"
     CDS             complement(2880..4028)
                     /locus_tag="VGD55_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0870068.1"
                     /translation="MRVVIAGAGFGGLELATLLSEEFGNDTDLVLIDKSEGFVFGFSK
                     LDVMFGRTSAAHVAHRYADVVRPGVRFVQSTVRSLDPVARRVTTDDGVFDADILVVAL
                     GADVDPSATPGLVEGGYEFYSVGGAFALRDVLASFAGGRVVIGVTSTPFKCPPAPSET
                     ALLMHDFLTARGLRDSSHISLVMPLGTPIPPSPDASAAILGAFAERGIDWHPGQSVRS
                     LDPGRKVALLSDGDELPYDLFLGVPAHRAPAVVVESGMAVDGWIPVNRLTLETQFPDV
                     YAVGDVTSVGTPKAGVFAEGQATVVAQQIIARVRGSSAASTYDGRGICYLEFGHDMVA
                     KVDVTFLAGQAPAGFLQGPSADLFADKAAFGSQRISRWFNRSWTPLDR"
     gene            complement(4050..4622)
                     /locus_tag="VGD55_06705"
     CDS             complement(4050..4622)
                     /locus_tag="VGD55_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870069.1"
                     /translation="MHQGRQSHPLTLARRVAGAALVSVAITACATNRSHTATDAPSQA
                     PVPSAPSFRSPTPTEPPTPALGSLTTPLPAGITTVKVTFLRGTRPVYEVSITDRATVQ
                     QIIGDVDDSHQDTGQTQGCAMRPVTMTLEFIAPSTDAVYSEDSGCARASLAIGGSQGP
                     LLDAALTDGIEQLLHVTFDLNGSPSVAPAS"
     gene            complement(4592..4780)
                     /gene="rpmB"
                     /locus_tag="VGD55_06710"
     CDS             complement(4592..4780)
                     /gene="rpmB"
                     /locus_tag="VGD55_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0870070.1"
                     /translation="MAAVCDVCGKGPGFGMSVSHSHRRTRRRWNPNIQTVRAVVGRGT
                     HKRLNVCASCIKAGKVTR"
     gene            4924..6435
                     /locus_tag="VGD55_06715"
     CDS             4924..6435
                     /locus_tag="VGD55_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEY0870071.1"
                     /translation="MIEVLDADAVRRWYRSGLRALEASRAEIDSLNVFPVADADTGTN
                     LFLTLQSAVAAADELDVRSADRADLAETATSMARLCLLGARGSSGVILSQLLRGIAEV
                     LAAERRPPRGLALTAAFEHAVTLAYAAVAQPVEGTMLTVARAAAEAARRTQSDRLDVV
                     VAAAVLAAHDALRATPSQLDVLSRAGVVDAGGRGLVVLLDVLQAVIDEREVILPAPRL
                     ASPTLDVGDSGSMSAAYEVMYLIDTTDDRVPALRAALDEIGEAVVVSGGDGLWNVHVH
                     TDDAAAAVELGVAAGRPHDIRVTEINEARAHHAETSERAVMIVLAAGSPALVLRDLLT
                     RSGAQVVETTAADPTSIGAALRKFGAREVVILVEHDGGLRAAVRSATAAPTAANAAIG
                     VIPIAAGVQFLSAVAVHDPHRRLADDVAAMETAASTTRVATAGPASFDAVCVDAVAQI
                     TQLLADGGDLVTVVSTAEVGGRIAGVLAETWPAVDVNHLTVEALGSVVWLGVE"
     gene            6435..8639
                     /locus_tag="VGD55_06720"
     CDS             6435..8639
                     /locus_tag="VGD55_06720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0870072.1"
                     /translation="MADLDAPLRPIVGTIAAVLAEKLDLHTVGDLMRHYPRRWEKRGE
                     LTDFKNLVAGEYATIFARIKKVSSRQITTKTNKRMNKTDVTVTDGSGEVVLTFFNQRG
                     WQLNRFPVGASAYFSGKVEWFNRKLQLVNPEVDVVEDDDDSRSEFAGQILAVYPAVKE
                     AGTVRLRRCVRLVLHDLDAPEDPVPQGIRDELGLMGYSEALEGIHRPNTFAEKDRARM
                     RLAWDEAFVLQIVLAQRRLAAAALPATARRPINDGLRAAFDARLPFELTQGQRDIGEV
                     IAAELAADHPMHRLLQGEVGSGKTVIALRAMLAVIDAGGQAALLAPTEVLAQQHHRSI
                     TQMLGDLALGGQLGGADVATTVTLLTGSMSQAARRKALLDAASGEAGIVIGTHALLED
                     RVQFADLGLVVVDEQHKFGVGQRDALRAKARDDRRPHVLVMTATPIPRTVAMTVYGDL
                     DVSTLVELPKGRSIITTSVAPVGGRPDWFKTVWQRVHEQVAAGHQAYVVCPRIGGGEP
                     DDDPLEPEDDLDDSATTTAAMPRRPPIGVLELAPRLADEYLDDLRIAVLHGQLPSDAK
                     DATMRAFADGTIDVLVATTVVEVGVDVANATAMVIMDADRFGISQLHQLRGRVGRGSA
                     PSRCLLVTELPAGHPGRDRLEAVATTSDGFELSEIDLQLRREGTILAGQQHGRSDLKL
                     LSLARDRDLIRSAREQANAIVEADPELTAHPPLAAAIQRLTATQDAEFIEKA"
     gene            8666..9277
                     /locus_tag="VGD55_06725"
     CDS             8666..9277
                     /locus_tag="VGD55_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY0870073.1"
                     /translation="MTRIVAGQAGGRRLAVPPSGTRPTSDRAREALFSSLESSLGSLK
                     GVRLLDLYAGSGAVGLEAWSRGAARVVLVESAAAAVKVLRMNVTSLRDARGTGSGAPG
                     LGPPVGKIEVLPVKAEKAATVLSASGFDVVFADPPYDLPAVQVAGVLATLAQRGVPRA
                     DAVVVVERATRDRWSWPLGYAATHDRKYGDATLHYGRYDPALL"
BASE COUNT         1530 a         3088 c         3113 g         1551 t
ORIGIN      
        1 agcaagccgt cgtgctccgc gcgtatgcca aatacctgcg ccaagccggc tatcgattca
       61 gccaggcgta tctcgagtca gccctgcgcg ccaacacaca cgtcgtccgt cttcttgtgt
      121 cgttgttcga aacgcgactc gacccggcct tccccggcga tcgagcctcg ggtgaaacgg
      181 cgattgcgca cgaactcgac ggtgctctcg atgcggtcac cagcctcgac gaagaccgga
      241 tcttgcggag cattgcgacg ttgatccggg cgacggtgcg cacgaactac tggcaacgct
      301 cggcggaggg tgcacccaag ggttatctct cgctcaagct tgactcgcaa gcagtgccag
      361 acctgcccga accgcggccg tgggtcgaga ttttcgtgta ctcaccgcag atggagggca
      421 ttcacctgcg gttcggaccg gtggctcgcg gcggcatcag gtactccgac cgccgcgaag
      481 atttccgcac agaggtgctt ggtctcgcca aaacgcaaac cgttaagaac gccgtgatcg
      541 tgccggtggg ttcgaagggc ggcttcgtcg tgaagcggcc gccggccgat gtgagtgatc
      601 gccgggcgat gcaggccgag gtggtggcgt gttaccgcac gctgatctgc ggcttgctcg
      661 atctgaccga caacctgatc gccgactccg agggccggcg taccgtgccg ccgaaagaca
      721 cggttcgcca cgatggcgat gatagctacc tggtggtggc ggccgacaag ggaaccgcga
      781 ctttttcgga catcgccaac ggaattgctg ctgagtatgg gttttggctc ggtgacgcgt
      841 tcgcgtctgg cggatctgcc ggctacgacc acaaggccat gggaatcacg gcgcgcggcg
      901 cttgggagtc agtgacacgg catttccgcg agctcggcgt caactgccag accacagatt
      961 tcacctgcgt cgggatcggc gacatgtcgg gcgatgtgtt cggcaacggc atgttgctgt
     1021 cgacgcacat ccggttgctg gctgccttcg atcaccgcca catcttcatc gatcccagcc
     1081 ccgatgcggc cgcgtcgttc gccgaacgcc gacgactgtt tgccctcgcg ggaagttcgt
     1141 gggccgacta cgacctggca ttgatctcgg ctggtggggg cgtatggccg cgcagcgcga
     1201 agtcggttcc gatctcggcc gagatggctg cggcgctcgg tgtcaaagcc gccgcgatgg
     1261 caccggccga tctcatgcgc gcgatccttc gggcgcccgc cgacctgttg tggaacggcg
     1321 gcatcggcac ctacgtcaaa gcgcacaccg agagccacgc cgacgtcggt gatcgcgcca
     1381 acgatgcgat ccgggtcgat ggccgcgagt tgcgatgccg ggtcgtgggt gagggcggca
     1441 acctcgggtt cacccaactc ggccggctcg aattcgcgct cggcggcggg cacatcaaca
     1501 ctgacgcgat tgacaactcg gcaggggtcg acacctccga ccacgaggtc aatatcaaga
     1561 tcctgctcga cggtgccgcg cgtgcgcacg agctcgacct tgccgaacgt ggacccttgc
     1621 tcgtgagcat gaccgacgag gtcggcgcgt tggtcttgcg cgacaactac gcccagaact
     1681 tggcgctgtc gtgttcgcaa acgcaagccc cgaccatgct gcacgtgcat gcggcctaca
     1741 tcgagtggct ggagaagaac ggccatctcg tacgggcgct cgaacatctc cctgacgccg
     1801 aagccatttc cgcacgccgg gcggccggcc tcggactcac tcggcctgaa ctcgcgctgc
     1861 tgctcgctta cgcgaaaatc accgccacca gagacatcgt cggctccgac ctacccgacg
     1921 atccgttcac caggcgtgcc ctcgtcgact acttcccgag cccgttgcgc gagcggttcg
     1981 ccaccgcgat gaccgaacat ccgctgcgcc gcgagatcat cgctacccag ttggccaatg
     2041 agctcgtgaa cttgtcgggc acctcgttct tgtaccgggt ggtctcagaa acggccgcct
     2101 cgacgtccga catcacccgc gcgcactcgg ctgcgcgcat gtcgtttcgg gtcgaccaga
     2161 tctggagcga catcgagggc ttggacgacg ttgtcgccag cgacgttcag accgagttgc
     2221 tgctgggcgt gcggcagctt ctagatcgcg cgacgcggtg gttccttgcg aatcggcggc
     2281 caccactcga cgtccccgaa actgttgatc agtggcagcc ccgaatcgcg gccgtgctcg
     2341 acgcgctgcc ccagcggttg cgtggtcgtg acgctgagaa ctatggagct gcgcttgggc
     2401 gctatgtcgg tgcgggcgta cccgattcga tcgccttggg ggcggtcgca ctgaccacag
     2461 ggttgtcggc actcgcgatc gtcgacatcg cgctgcaaac caagcgcgac gtcgacgagg
     2521 tggctggcgt gcacttcgcg atcgccgacg tcctcgggct ctcacggctc ggttcgctca
     2581 tcgcggcgct acctcgcgac agtcggtggc acacgctcgc gcgcgccgct gcccgcgacg
     2641 accttgaggc cgcgcacgcc gaactcacga ccgatgtgtt gctgtcgacg gggccgaccg
     2701 ccactgctga cgaccggatc tctgcgtggc agcaggcaaa tggcgcggcg ctcgcgcggg
     2761 cgacatcctt catcgccgac atcagcgtga gtgatggcgc cgacctcgcg acgctgtcgg
     2821 tggccctgcg cgaagtgcgc gcgctcgccc gcgcggctgc cctcccgcac gcgtagcggt
     2881 cagcggtcga gcggggtcca gcttcggttg aaccagcggc tgatgcgctg cgagccgaac
     2941 gcggccttgt cggcgaaaag gtcggctgac ggaccttgga ggaatccggc aggcgcctgg
     3001 ccggcaagga aagtcacgtc gaccttcgcc accatgtcgt gcccgaactc gaggtaacag
     3061 atgccccgac cgtcgtaggt gctggccgca ctcgaaccgc gtacgcgcgc gatgatctgc
     3121 tgcgcgacca cggttgcctg gccttcggcg aagaccccgg ccttgggcgt tccgacgctg
     3181 gtgacgtccc caaccgcgta tacgtcggga aactgcgtct caagcgtcaa ccggttcacc
     3241 ggtatccatc catcgactgc catgcctgac tcgacgacga ccgctggagc acgatgggcc
     3301 gggacgccga ggaagagatc gtaaggcagt tcatcgccgt cactcaaaag ggcaaccttg
     3361 cgccccggat caaggctgcg caccgactgt cctgggtgcc agtcgatgcc ccgctccgcg
     3421 aacgcgccga gtatcgccgc cgacgcgtca ggcgacggcg gaatcggcgt cccgagcggc
     3481 atcaccaacg agatgtgcga ggagtcgcgc aacccccgtg ccgtcaggaa gtcgtgcatc
     3541 aacagcgcag tctcgctcgg cgccgggggg catttgaacg gtgtcgacgt cacgccgatg
     3601 acaacgcgtc cgccagcaaa gctcgccaag acatcgcgca gcgcgaaggc cccgccaacg
     3661 ctgtagaact cgtagccgcc ttctacgagc ccgggagtcg ccgacggatc aacgtccgcg
     3721 cccagggcca ctaccaggat gtcggcgtcg aacacaccgt cgtcggttgt cacgcgtcgc
     3781 gcgacggggt cgagtgagcg cactgtcgat tgcacgaaac gaacaccggg ccggacgacg
     3841 tcggcgtagc gatgcgcaac atgcgcggcg ctcgtacggc cgaacatcac gtcgagcttg
     3901 gagaacccga agacgaaccc ttcggacttg tcgatcagca ccaggtcggt gtcgttgccg
     3961 aactcctccg acaacagcgt cgcaagttca agcccgccga accccgcgcc tgcaatgacg
     4021 acccgcatcc tcgcagccta gaacctgccc taggaggctg gcgccaccga cggcgaacca
     4081 ttgaggtcga aggtgacgtg aagcagctgt tcgattccgt ccgtcaacgc ggcgtcgagg
     4141 agcgggccct gcgaaccgcc gattgccagg ctcgcccgcg cgcagccgct gtcttccgag
     4201 tacacggcgt cagtcgacgg tgcgatgaat tcgagagtca tcgtgaccgg ccgcattgca
     4261 cagccctgcg tctggccggt gtcctggtga ctgtcgtcga cgtccccgat gatctgctgg
     4321 accgtggccc ggtcggtgat cgacacctca tagaccggcc tggtgccccg aaggaacgtg
     4381 accttaaccg ttgtgatgcc ggcgggcagc ggcgtcgtca gcgagccgag cgccggggtc
     4441 ggtggctcgg ttggcgtcgg gctccgaaag ctcggcgcgg acggaacggg cgcttgggac
     4501 ggcgcgtcgg tggcggtgtg cgagcgatta gtggcgcatg ccgtgatggc cacgcttacc
     4561 agcgccgccc cggcgacgcg gcgcgcgagc gtcagcgggt gactttgccg gccttgatgc
     4621 acgaagcgca cacgttcaag cgcttgtggg tgccgcgtcc gacaaccgcg cgcacggtct
     4681 gaatgttcgg gttccagcgc cggcgggtgc gccggtggga gtgggacacg gacatgccga
     4741 accccggacc cttgccgcag acatcgcaga cagcagccac gggagacact ccaagcatga
     4801 ggtgggcggc cgcaggatcg atcgcagccg cagatttcat tcggacaacc gcacgagact
     4861 atccgatggc ccgccggtca cgccaaccgg ccggtgtcgc cacggctggt tagcctcctc
     4921 cttgtgattg aggtcctcga tgccgatgcg gtccgccggt ggtaccggtc ggggctgcgc
     4981 gcgctcgagg cgagccgggc cgagatcgac tccctcaacg tcttccccgt cgccgacgcc
     5041 gacaccggca cgaatctctt cctgacactc cagagcgcgg tcgcagcggc cgacgagctc
     5101 gacgtgcgca gcgccgaccg agccgatctg gccgagaccg cgaccagcat ggcaaggctt
     5161 tgcttgctcg gggcccgcgg cagctcgggc gtgatcttga gccagctgtt gcgcgggatc
     5221 gccgaggtgc tggccgccga gcggcgtccg cctcgaggtc tggccctaac cgcggcattc
     5281 gagcatgcgg tgaccctggc gtacgcggcg gtcgcccaac ccgtcgaggg caccatgctc
     5341 accgtcgccc gcgctgcggc cgaagccgct cgtcgcaccc aatctgatcg gctcgacgtg
     5401 gtcgtggcgg cggccgtgct cgcggcgcac gacgcgctgc gagcaacgcc gtctcagctc
     5461 gacgtgctga gccgcgccgg agtggtcgac gcgggcggca gaggcctcgt cgtgttgctc
     5521 gatgttttgc aggccgtgat cgacgagcgc gaggtcatct tgcccgcgcc tcggctggcg
     5581 tcgccgaccc tcgatgtagg cgattcgggc tcgatgtcgg cggcatacga ggtcatgtac
     5641 ctcatcgaca ccaccgacga ccgcgtcccg gcgttacggg cggcactcga cgagatcggc
     5701 gaggccgttg tcgtctctgg cggcgacgga ttgtggaacg tgcacgtgca caccgacgac
     5761 gccgctgcgg cggtcgagct cggagttgcc gccggtcgac cacacgacat cagggtcacc
     5821 gagatcaacg aggcgcgggc tcaccacgct gagacgtccg aacgcgcggt catgatcgtg
     5881 cttgccgcag ggtcccctgc gctggtcctt cgcgacctgc tcacgcggtc gggtgcgcag
     5941 gtagtcgaaa caacggcagc agatccgacg tcgatcggtg ccgcgctgcg aaagttcggc
     6001 gcgcgcgagg tggtgatcct cgtcgaacat gacgggggcc tgcgagccgc ggtgcggtcg
     6061 gcaactgcag ctccgacggc ggcgaatgcg gccatcgggg tgatccccat cgccgcgggc
     6121 gtgcagttcc tgtctgcggt cgctgtacat gacccgcacc gacggctcgc cgacgatgta
     6181 gccgcgatgg agacggccgc ctcgaccact cgcgtcgcga ccgccggccc cgcctctttc
     6241 gacgcggtgt gtgtcgacgc tgtcgcccag ataacgcagc tgcttgccga cggcggcgac
     6301 ctcgtgacag tcgtgtcaac ggccgaggtc ggcgggcgta ttgccggtgt cctggccgag
     6361 acctggcccg ctgtcgacgt caatcacctg accgtcgagg cgctcggttc ggtggtctgg
     6421 ctcggggtcg agtgatggcg gatctcgatg ctccgctgcg accgatcgtc ggcacgattg
     6481 ccgcggtgct ggcggaaaag ctcgatctgc acacggttgg cgatctcatg cggcactatc
     6541 ccaggcgctg ggagaagcgc ggtgagctga ccgacttcaa gaatctggtc gcaggtgaat
     6601 acgcaacgat cttcgcccgc atcaagaagg tgtccagcag gcagatcacc accaagacca
     6661 acaagcgaat gaacaagact gacgtcaccg tgaccgacgg ttctggcgaa gtcgtcctca
     6721 cctttttcaa ccagcggggt tggcagctga accgcttccc tgtgggggcc agtgcctact
     6781 tctcgggcaa ggtcgagtgg ttcaatcgca aactccagct ggtcaatccc gaggtcgacg
     6841 tcgtcgagga cgacgacgac agtcggtccg agttcgccgg gcagatcctc gcggtttatc
     6901 cagcggtcaa ggaagccggc accgtcaggt tgcgccgatg cgttcggctg gtgctccacg
     6961 atcttgatgc accggaagac cctgtgcctc aagggattcg cgacgagctc gggctgatgg
     7021 ggtacagcga ggccctcgaa ggaatccatc gcccgaatac atttgccgag aaagaccgcg
     7081 cgcgcatgcg cctcgcgtgg gacgaagcgt tcgtgcttca gattgttctt gcgcaacgtc
     7141 ggttggccgc cgccgcactg ccggccactg ctcggcgtcc gatcaatgac ggattgcgcg
     7201 cggccttcga tgcgcgactg ccgttcgaac taacccaagg acagcgagac attggcgagg
     7261 tgattgcggc cgagcttgcc gccgaccatc cgatgcacag gttgcttcaa ggtgaggtcg
     7321 ggtcgggcaa gacggtcatc gcgttgcgtg cgatgttggc ggtcatcgac gcgggcgggc
     7381 aggccgcact gctcgcaccg acagaggtgc ttgcccagca gcatcatcga tcgatcaccc
     7441 agatgctcgg cgacctcgcg cttggtggcc aacttggtgg cgccgatgtc gcgacgactg
     7501 tcacgctgct caccggatcg atgagtcagg cggctcgacg caaggcattg ctcgatgccg
     7561 catctgggga ggcgggcatc gtcatcggca cgcacgcgct gctcgaagac agggtgcagt
     7621 tcgccgatct tggcctcgtc gtggtcgacg agcagcacaa attcggcgtc gggcaacgcg
     7681 acgcgcttcg cgcgaaggcc cgcgacgacc gccgcccgca cgtgctcgtc atgaccgcga
     7741 caccgatccc gcgcacggtc gcaatgaccg tctacggcga ccttgacgtg tcgacgctgg
     7801 tcgagctccc gaagggccgc tcgatcatca ccacctcggt ggcgccggtc ggcggccgtc
     7861 ccgactggtt caagaccgtg tggcaacgag ttcacgaaca ggtcgccgcg ggccatcagg
     7921 cctacgtcgt ctgtccgcgc atcggcggcg gcgagcccga cgacgatccg ctcgagcccg
     7981 aggacgacct cgacgacagc gcgacgacca ccgccgctat gccgcgacgc ccgcccatcg
     8041 gcgtcctcga acttgctcca cggcttgccg atgaatacct cgacgacctg cgcatcgcgg
     8101 tcctgcacgg tcagctgccc agtgacgcga aggatgcgac gatgcgcgcg ttcgccgacg
     8161 gcacgatcga cgtcctggtg gcgacgaccg tggtcgaggt cggggtcgac gtcgcgaatg
     8221 ccaccgcgat ggtgatcatg gacgccgacc ggttcggcat ctcgcaactt caccaactcc
     8281 gtggccgggt ggggcgcgga agtgcgccga gtcgctgcct gctcgtgacc gagttgccgg
     8341 ccggccatcc gggacgcgac cgactcgagg cggtagccac gacgtccgac ggtttcgagc
     8401 tttccgaaat cgaccttcag ctccggcgcg agggcacgat cctggccggt cagcagcacg
     8461 ggcgcagcga cctgaagctg ctctcgctgg cacgcgaccg cgacctcatt cgctccgcgc
     8521 gcgagcaggc gaacgcgatc gtcgaagcag atcctgagct gaccgcgcac cccccgctcg
     8581 cggccgcgat ccagcggctg actgccacgc aggacgccga gttcatcgag aaggcctaag
     8641 tggccaaacg ccgctggcgc gcgcgatgac caggatcgtc gccggtcagg caggcgggcg
     8701 gcgcctcgcg gttccgccgt cgggcacccg accgacgtcc gatcgtgccc gcgaggcgct
     8761 tttctcgagc ctcgagtcgt cactcggcag cttgaagggg gtccggttgc tcgatctcta
     8821 cgccggctca ggcgcggtcg ggctggaagc gtggtcgcgg ggggcggcgc gcgtggtgct
     8881 cgtcgagtcg gccgccgcgg cggtgaaggt gttgcggatg aacgtgacca gcctccgcga
     8941 cgcccgcggc actggaagcg gtgcgcccgg actcggtccg cctgttggga aaatcgaggt
     9001 gctgccggtc aaggccgaga aggccgcgac agtgctttcg gcgtccggct ttgatgtcgt
     9061 ttttgccgac ccgccctacg atctgccggc ggtgcaggtg gcaggagtgc tggccacgtt
     9121 ggcgcaacgg ggtgtgccgc gagccgatgc ggtcgtcgtg gtcgaacggg cgacccgcga
     9181 ccgttggtcc tggcctcttg gctacgcggc gacccatgac cgcaaatacg gcgacgcgac
     9241 gttgcactac ggccggtatg acccggcctt actgtagaag cg
//
LOCUS       DASWVF010000374         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_264844, whole genome shotgun sequence.
ACCESSION   DASWVF010000374 DASWVF010000000
VERSION     DASWVF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264844"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1215
                     /locus_tag="VGD55_06730"
     CDS             94..1215
                     /locus_tag="VGD55_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870074.1"
                     /translation="MSNPYHYGTPVSGDQFAGREQETAGLVARMRDGINVVVISPRRY
                     GKTSLLARAAERMRADGAAVVEINALRCRDLAAFAAQLANHTYRLKGGRWHRVRQAVP
                     EFLRRLRVSPSVTFEGDQPRFSFSSTLSTEDAETVIADVYALLEELSERKPAVLVLDE
                     FQAITTLGAHLPSLFKALADANPHVSLVLEGSKRHLMERLVVSADAPLFGMAERFALD
                     VLPDDVMTAYLRARADVGGKPMSQAAADRLVELAGPVPNDVQQLAYEVFNVAERRITV
                     DDVEQGLLMAVEHEATLHADRFESLAPGQRRVLSELARSPAREPYSASFARDVALATA
                     GSVRTALDALIADELVSFRGGTYRVGNPFFAAWLRESLS"
     gene            complement(1142..1726)
                     /locus_tag="VGD55_06735"
     CDS             complement(1142..1726)
                     /locus_tag="VGD55_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870075.1"
                     /translation="MVPDSFSGYFAAAAAAAGALIGLLFVAVSLRPDDIFGDSTPGGG
                     KALATSSFTGLVNGFFLSMAALIPGSNLGIPAAVLAVLCLSSTARLHRSVGAMRSSVL
                     TSVLSIAVFVFQLVAGIGLVTRPHDEVFVKELAWVTITSFTVALLRAWSLLKGKASSA
                     EKARSTSSDEVMTGSRATTRRRTGFPPGTSHRES"
     gene            complement(1846..2427)
                     /locus_tag="VGD55_06740"
     CDS             complement(1846..2427)
                     /locus_tag="VGD55_06740"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEY0870076.1"
                     /translation="MVLIAAVLVATVPLFGGRLRSLAQLRIRSFWLIASALALQVLAI
                     SVVPTWPHALLVAAHLVSYVMAGWFVWLNRRISGLPIVALGGALNALAIAANGGTLPA
                     SRSALAAAGIHPVAGDFNNSGVLAHPHLAWLGDVLAVPAAVPMANVFSIGDVLILVGF
                     AYGLHHLCGSRLVRGSRRVREGLSTASPSAVPQ"
     gene            complement(2449..>3749)
                     /locus_tag="VGD55_06745"
     CDS             complement(2449..>3749)
                     /locus_tag="VGD55_06745"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY0870077.1"
                     /translation="DTLGHHIGDQVIESTARRLSAALGANAMVSRLGGDEFAVLVPDI
                     EPSAVLIVAHVALRALAAPLTADGVRIDVGASIGVAVAPEHGLTVSTLLRRADVALYT
                     AKEERNSVRIYSLDDDRNTVERLELLGDLRGAIERGEVVVHYQPKIAALTGGVVGVEA
                     LARWEHPGRGLIMPDEFIGLAEHTGLLSLLTDCVLRNGLADLRRWRTVEPTLTLAVNV
                     APAQLNDREFPESVWVALHRAGVPAAALTLEVTENGVMASSGRVRQVLDDLRRMGVQL
                     SIDDFGSGSTSLSYLGRLPLSELKIDKRFVIGLDDPINHAIVRSTIELGHNLGMQVVA
                     EGVETLDVWHELQVLGCDILQGHAIARPLPSIAMTSSLASRRTEGVDFSLLAHRTAAP
                     SLDAPLIGAASVGAPSIGGPLLVVAPDVSQASAPPVSTAH"
BASE COUNT          706 a         1270 c         1221 g          552 t
ORIGIN      
        1 accagccgcc acctccctaa ccgccgccca gttgctcgtc tacgctatgc tagttaacat
       61 gatgtcgact tacaacatag cgaagagacg ttgatgtcga atccgtacca ctacggcacc
      121 ccggtgtcgg gcgaccagtt cgccggccgc gagcaggaga cggccggcct ggtggctcgc
      181 atgcgcgacg gcatcaacgt ggtcgtgatc tcccctcggc gctacgggaa gacctctctg
      241 cttgcgcgcg ccgccgagcg catgcgtgcc gacggcgccg ccgtggtcga gatcaacgcg
      301 ttgcgatgcc gcgatctcgc agcgttcgcg gcgcagctcg ccaaccacac gtatcggctc
      361 aagggtggcc gctggcaccg ggtccgccaa gcagtgccgg aattccttcg ccggcttcgg
      421 gtctcaccgt ctgtgacgtt cgaaggagat caacccaggt tcagcttctc ctcgaccctg
      481 tcgaccgagg atgccgagac cgtcatcgcc gacgtctacg cgctgctcga ggagctcagc
      541 gagcgaaagc ctgccgtcct cgtcctcgac gagttccaag caatcaccac actcggggct
      601 cacctgcctt cgttgttcaa ggcgctcgcc gacgccaatc cacacgtgtc gctggtgctt
      661 gaaggctcga agcgacacct gatggaacgc cttgtcgtct ctgccgacgc gccactgttc
      721 gggatggcgg aacggttcgc actcgacgtc ctgcccgacg acgtcatgac tgcgtacctg
      781 cgcgcacgag ccgacgtcgg cggcaagccc atgtcgcagg cggccgccga ccggctggtc
      841 gaactcgccg gaccggtacc gaacgacgtc cagcaactcg cttacgaagt cttcaacgtc
      901 gccgagcgac gcatcacggt cgacgacgtc gagcaaggac tgctgatggc cgtcgaacac
      961 gaggcaacgc tgcacgccga ccgcttcgaa tcgctcgccc cgggccagcg ccgcgtgttg
     1021 tcggaactcg cacgcagccc cgcacgtgag ccgtattcgg ctagcttcgc gcgcgatgtc
     1081 gccctcgcga cggccggatc cgtgcgcacc gccctcgacg cactcatcgc cgacgaactc
     1141 gttagctttc gcggtgggac gtaccgggtg ggaaacccgt tcttcgccgc gtggttgcgc
     1201 gagagcctgt catgacctca tcggacgacg tcgagcgggc cttctcggcg gacgatgcct
     1261 ttcccttcag tagggaccac gctcgcagca gcgcaaccgt gaacgacgtg atcgtcaccc
     1321 atgccaactc tttgacgaag acctcatcgt gaggccgcgt caccagcccg atgccagcaa
     1381 cgagctgaaa cacgaagacc gcaatcgaaa gcaccgaggt caacacgctc gaccgcattg
     1441 ctccgacgct gcgatgcaat cgcgcagttg aggacaggca gaggacggcc agaaccgccg
     1501 ccgggatacc gagattgctc cccggaatga gcgcggccat cgacaggaag aatccgttca
     1561 ccaagcccgt gaacgagctc gtggccagcg ccttcccgcc gcctggcgtg gaatcgccaa
     1621 agatgtcgtc gggacgcagc gaaaccgcca cgaacagcag gccgatgagg gcgccggccg
     1681 cagcggccgc agctgcgaag tagccagaaa agctgtcggg aaccaccggc gtcctcgtcg
     1741 tatgcgcagg tcagcgtgaa aggcggcccc gatcgtagcg ccgccgcaga gcaaagcgcg
     1801 gcgaagagtg tctcccgcgg cacggccaga cgcgctaaag accgcttatt gagggacggc
     1861 ggacggggaa gcggtcgaga gtccttcgcg gacccggcgc gagcctcgca cgaggcggct
     1921 gccgcacagg tgatgcagtc cgtacgcgaa gccgacgaga atcagcacgt cgccgatgct
     1981 gaacacattg gccatcggga cggcagcggg aaccgcgagt acgtcgccta gccacgcgag
     2041 gtgcgggtgc gcgagcacgc ccgagttgtt gaagtcaccg gcaaccgggt ggatgcccgc
     2101 tgccgcgagc gccgaccgcg acgccggcag cgtgccgccg ttagccgcaa tcgccaacgc
     2161 gttgagggca ccgcccagag cgacgatggg cagtccactg atgcgtcggt tcagccagac
     2221 gaaccatccg gccatcacgt aactgaccag atgcgcggcc acgagcaggg cgtgcggcca
     2281 ggtcggcacc acgctgatgg caagtacctg caaagcaagc gccgacgcga tcagccagaa
     2341 cgagcggatg cgcaactgcg cgagtgatcg cagccggcca ccgaacagcg gcaccgtggc
     2401 gacaagcacg gcggcgatca agaccatgag cacgacgaac tccctggctc agtgggcggt
     2461 gctgacgggg ggtgcggagg cctggctcac atcgggcgcg acgacgagca aaggcccacc
     2521 gatagacggc gcaccaacag acgccgcacc gatcaacggt gcgtccagtg agggagccgc
     2581 ggtgcggtgc gccaacaacg agaagtcgac gccttcggtg cggcggcttg cgagtgacga
     2641 cgtcatggcg atcgatggca acggccgcgc gatcgcatgg ccctggagga tgtcgcagcc
     2701 cagcacctgc agttcgtgcc acacgtcgag tgtctcgacg ccctcggcca cgacctgcat
     2761 gccgaggttg tggcccaact cgatggtcga ccgcacgatc gcgtggttga tggggtcgtc
     2821 caacccgatc acgaaccgct tgtcgatctt caactcgctc agtggcaacc ggcccaggta
     2881 agacagcgac gtcgacccgc tgccgaagtc gtcgatcgag agctgcactc ccatccgtcg
     2941 taggtcatcg agcacctgac gaacgcggcc actcgaggcc atcacgccgt tctcggtcac
     3001 ctcgagggtg agcgccgcgg ccggcacgcc cgcgcggtgc agtgcgaccc acaccgattc
     3061 ggggaactcg cggtcgttga gttgggcggg ggcaacgttg acggcgagcg tcagcgtggg
     3121 ctcgacggtc cgccagcgtc gcaggtcggc gagcccgttg cgcaggacgc agtcggtgag
     3181 cagcgagagc aatccggtgt gctcagcgag accgatgaac tcgtcgggca tgatgagacc
     3241 gcgacccggg tgttcccagc gcgcgagtgc ctcgacaccg acgacgccgc cggtgagtgc
     3301 cgcgatcttc ggctggtagt gcacgacgac ctcgccgcgc tcgatcgcac cgcgcagatc
     3361 accgagcagc tcgaggcgct cgacggtgtt gcggtcgtcg tccaacgaat agatgcgtac
     3421 tgagttgcgc tcttctttgg ccgtgtaaag ggcaacgtcg gcgcgccgca gcagcgtcga
     3481 cactgtgagt ccgtgttcgg gagccaccgc gacgccgatg ctcgcaccga cgtcaatgcg
     3541 cacgccgtcc gccgtcaatg gcgcagccaa cgcacgcagc gcaacgtgcg cgacgatcag
     3601 gaccgccgag ggctcgatgt ccggcacgag tacggcgaac tcgtcgccgc ccaagcggga
     3661 aaccatcgcg ttcgcgccga gcgcagccga cagccgccta gcagtcgact cgatcacctg
     3721 gtcgccgatg tggtggccga gtgtgtcgt
//
LOCUS       DASWVF010000375         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_86191, whole genome shotgun sequence.
ACCESSION   DASWVF010000375 DASWVF010000000
VERSION     DASWVF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86191"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGD55_06750"
     CDS             complement(<1..272)
                     /locus_tag="VGD55_06750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870078.1"
                     /translation="MPGAAGAWEAVPDVEKGPEVRGAAAATRVKIVIPAHDEAAVVDR
                     CLSAVLASARPGEFDVVVISNGSTDDTVARARAAGARLAHEVEVVE"
     gene            417..1418
                     /locus_tag="VGD55_06755"
     CDS             417..1418
                     /locus_tag="VGD55_06755"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0870079.1"
                     /translation="MTQLSEPATLADPTNEATQATESPRIALGGDRKSGLEQFALGVF
                     VVVPFLALLAAIPVFWGWGLGWHDIVIAVVMYAIAGHGITIGYHRLFTHKSFKAARPL
                     RVALAFAGGMAIEGPVVRWVADHRRHHAFSDKEGDPHSPWRFGTSIGALIKGLLYAHV
                     GWIFDPEQTDATRYAPDLIADRDIVAVSRLFWLQVTTSLLLPALIGGLWSMSIAGAVT
                     GFFWGALVRISLLHHVTWSINSICHAVGERPFRTRDKSGNVWWLSVLSMGESWHNLHH
                     ADPTSARHGVLRGQLDSSGRTIWLFEKFGWARDVRWPSPERIKARLVREREREPSSV"
BASE COUNT          280 a          601 c          566 g          310 t
ORIGIN      
        1 atctcgacga cctcaacctc gtgcgcaaga cgtgcacccg cagcgcgcgc ccgcgccacg
       61 gtgtcgtcgg tactgccatt gctgatgacg acgacgtcga actcaccggg gcgagcggag
      121 gcaaggaccg cagacaggca ccggtcgacg acggcggcct cgtcgtgcgc cgggatcaca
      181 atcttgacgc gggtggctgc ggcggcgccg cgaacctcgg gccccttctc aacgtcgggc
      241 actgcttccc acgctcccgc tgctcccggc attccctgat ccgcatggcg cgctcggctg
      301 acgtcttacg ctagcgaaag taagggtggc cgcgtggctg gatgcgtgag ttcagccatc
      361 tggccgcacg ggcggttcgc atcgggtgcg cgcggaccgt aggttaccgt gaccccatga
      421 cgcagctgtc cgaacccgcg accctcgcag accccactaa cgaggcgacc caggcgaccg
      481 aatcgccgcg catcgcactg ggcggcgacc gtaagtctgg tctcgagcag ttcgcactcg
      541 gcgtcttcgt cgtcgtcccg ttcctggccc tactcgccgc gatcccggtg ttttggggtt
      601 ggggcctcgg ctggcacgac atcgtcatcg ccgtcgtcat gtacgcgatc gccggtcatg
      661 gcatcacgat cggctaccac cggctcttca cgcacaagtc gttcaaggcg gcccggccgc
      721 tgcgggtcgc gctggcgttc gccgggggca tggccatcga aggcccggtc gttcgctggg
      781 tcgcagacca ccgccgccac cacgcgttca gcgacaaaga gggtgacccg cattcgccgt
      841 ggcgtttcgg caccagcatc ggcgcgctga ttaagggcct gctgtacgcc cacgttggtt
      901 ggatcttcga ccccgagcag accgacgcca cccgctacgc gcccgacctc atcgccgacc
      961 gcgacatcgt cgcggtgtcg cggctgttct ggcttcaggt cacgacgtcg ctgctgctgc
     1021 ccgctctcat cggtgggctg tggagcatgt cgatcgccgg ggccgtcacc ggcttcttct
     1081 ggggcgcgct cgtgcgcatc tcattgctgc accacgtcac gtggtcgatc aactcaatct
     1141 gccacgccgt cggtgagcgt ccgttccgca cccgcgacaa gtcgggcaac gtgtggtggc
     1201 tctctgtgct gtcgatgggg gagtcgtggc acaacctgca ccacgcggat cccacgtcgg
     1261 cacgtcatgg cgtgctgcgc ggtcaactcg attcgagcgg gcgcacgatc tggctgttcg
     1321 agaagttcgg ctgggcgcgc gacgtgcgct ggccctcacc cgaacgcatc aaagcccgcc
     1381 tggtccgcga gcgcgaacgc gaaccttcgt cggtttgaat cgcggctgcc gaggccacgc
     1441 actcggcgac tttgttggtc tcgattagtc gaagaaggct agccagacca cgatcgccca
     1501 gaacgccagg tcgaacacca agccggcgac gaaactcggg caggcatggc ctaagtgccg
     1561 acgtcagcga tcgatggcgg cgcacgtagt tggccgcacc cctttcgatg cgagcacatc
     1621 gtcgcgtgcg ctcacgccac cgtccgtcaa gtcgccacag tgcaagaaga agcggcagcc
     1681 ctgggcaggg aatcgtggta cagatgatcc attaggggcc gatgcgtgac agggatcctc
     1741 acgatgttgt tacggct
//
LOCUS       DASWVF010000376         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_111846, whole genome shotgun sequence.
ACCESSION   DASWVF010000376 DASWVF010000000
VERSION     DASWVF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111846"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="hpnC"
                     /locus_tag="VGD55_06760"
     CDS             <1..849
                     /gene="hpnC"
                     /locus_tag="VGD55_06760"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY0870080.1"
                     /translation="AKARSENFPVAAWFLPRRLRADLLAIYGYARFVDDIGDETDVDQ
                     LTRLAMLDLVDADVTTLFAGGDATLPAVAALTAGVREGRVPELPLRRLVDANRLDQRA
                     DRYETFADLRAYCVLSADPVGHLVLAALGLATPGRLELSDRVCTALQLAEHWQDVAED
                     LARGRIYLPQEDLRRFGVTDDDLRAEHAGPAVKTLMAFEVARARDLLGRGAPLVRLVP
                     GRAKLAIAGFVAGGRAALHAIAAADFDVLGGAPKATKERLMREALGVLVSRPKRTIGR
                     KRPGRR"
     gene            846..1724
                     /locus_tag="VGD55_06765"
     CDS             846..1724
                     /locus_tag="VGD55_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0870081.1"
                     /translation="MTTENHEADVPAAYAECERIMVKAAKNFSYGIRLLAPAKRGALA
                     AVYALARRIDDIGDGTLPAQEKLRLLDDARRDIKSIGGPTTDPVLLAMGDAAARLPIP
                     VAAFLELVDGCELDVRGATYETFPDLVYYCRCVAGSIGRLSVGVFEASDPVAADPLAD
                     ALGVGLQLTNILRDVREDLQNGRIYLPREELEASGVELRIDANGHVADDGGRTSMLIR
                     EQAARAEKWYAEGLQLLPLLDSRSAACCSAMAGIYHRLLKRIARNPQAVLHERISLPA
                     WEKAYLAARSVTKSRR"
     gene            1721..3109
                     /gene="hpnE"
                     /locus_tag="VGD55_06770"
     CDS             1721..3109
                     /gene="hpnE"
                     /locus_tag="VGD55_06770"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY0870082.1"
                     /translation="MIDKPTLVVIGGGVAGLTAAVTAADAGWAVTVLEARARLGGATH
                     SFTRAFDDGEVIVDNGQHVFLRCCTAYREFLHRLGVENDTVMQSRLDVPVFDPTTGRG
                     ARLRRDRLPAPLHLSRALLRYRLLTPMQRVRAIVAALMLNRVDRNAPSSDAQTFRSWL
                     KAHGQSDAAIERLFDVFTVATLNAPAAQVSLSLGAMVFQDGLLREADACDIGMARVPL
                     GQLHGDAAAAALSGHDIKMRTPVRAVTQLPGNRWQVETDDASIEADAVVLAVPHDVAA
                     DLLPAGAIADPDVVRGLDVAPILNVHVIFDRVVLTEPFIAAVGSPAQFVFDRTAPSGL
                     REGTGQYVAVSVSAADQWIDAPVARLREVFMPELRKLLPAARDAHVLDFFVTREREAT
                     FRPVPGSAARRLGPATALPGLVVAGAWTATGWPATMESAVRSGLAAIDELQKSRRTSP
                     ADSRDVSQQASA"
     gene            3106..>3915
                     /locus_tag="VGD55_06775"
     CDS             3106..>3915
                     /locus_tag="VGD55_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0870083.1"
                     /translation="MTTAAGHPPSPPPSGLPDPDVQVVLTRSRDLTDPPMREAVGRLA
                     PAMRDVAAYHLGWVDADGSESETHRGKSVRPALAMLSAEAVGAPPADSIPGGLAVELV
                     HNFSLLHDDVMDGDTERRHRPTAWAVFGSSAAILAGDSLLSLAFEALLDVEPVRAHPA
                     QRSLAAATQRLIAGQIGDLEFERRLDVTLDECLEMAAGKTAALIACSASIGAHLGGGP
                     TPVISALEGFGYELGLAFQLVDDLLGIWGSSASTGKPVGADLRARKNSLPVV"
BASE COUNT          592 a         1388 c         1293 g          642 t
ORIGIN      
        1 gccaaggcgc gctccgagaa cttcccggtc gcggcctggt tcctcccccg ccggttgcgg
       61 gcggacctgc tcgccatcta cggctacgcg cgcttcgtcg acgacatagg cgatgagacg
      121 gacgtcgatc agctgacgcg actcgcgatg ctcgacctcg tcgacgccga cgtcacgacc
      181 ctgttcgcgg gcggcgacgc gacgctgccg gcggtcgcgg cgctgaccgc gggcgttcgc
      241 gagggtcggg tacccgaact cccgctgcga cggttggtcg acgcgaaccg gctcgaccag
      301 cgggccgacc gctacgagac cttcgccgac ctgcgggcgt actgcgtgct ctcagccgac
      361 ccggttgggc acctcgtgct cgcggcgctc ggccttgcca ccccgggtcg cctcgagctg
      421 tccgaccgcg tctgcaccgc cctccagctt gccgagcatt ggcaagacgt cgcagaagac
      481 ctggcacgtg gccgcattta cctaccgcaa gaggacctgc gccggttcgg ggtgactgat
      541 gacgacctgc gcgccgagca tgcagggccg gccgtgaaga ccctgatggc gttcgaagtt
      601 gcgcgagccc gtgacctcct cgggcgaggc gcgccgctcg tgcgactggt gcccggccgg
      661 gcaaagctcg cgatcgccgg cttcgtagcg gggggccgcg ccgcactgca cgcgatagcg
      721 gccgccgact tcgacgtcct cgggggcgca ccgaaagcga ccaaggaacg actgatgcgc
      781 gaggctctcg gcgttctcgt gagccggccc aagcgcacga tcgggcgcaa acgaccgggc
      841 cggcgatgac cacggaaaac cacgaagccg acgtgcccgc tgcgtacgca gaatgcgaac
      901 gcatcatggt caaggcggcg aagaacttct cctacgggat acggcttttg gcgcccgcca
      961 agcgcggagc gctggccgcg gtgtacgcgt tggcccgccg catcgacgac atcggcgacg
     1021 gcacgctgcc cgcgcaggag aaactgcggc tcctcgacga cgcccggcgt gacatcaaat
     1081 ccatcggcgg ccccacgacc gaccccgtct tgctcgccat gggcgacgcg gcggcccgac
     1141 tgccgattcc ggtagcggca ttcctcgagc tcgtcgacgg ctgcgagctc gacgtccggg
     1201 gcgcgaccta tgagacgttc cccgacctcg tgtattactg ccggtgtgtc gccggttcga
     1261 tcggccggct ctcggtcggt gtcttcgagg cgtcagatcc ggtcgcggcc gatccccttg
     1321 ctgacgctct cggagtcggt ctccagctca cgaatatcct ccgcgatgtg cgcgaggatc
     1381 ttcaaaacgg gcgcatttac ctgccgcgcg aagaactgga agcatcgggg gtcgagcttc
     1441 gtatcgatgc gaacggacac gttgccgacg atggtggccg cacctcgatg ctgatccgcg
     1501 aacaggctgc ccgcgccgag aagtggtacg cggagggtct ccaactgctg ccgctgctcg
     1561 actcgcgaag cgcggcatgt tgctcggcca tggccggcat ctaccaccgg ctgctcaagc
     1621 ggatcgcccg caacccgcaa gccgtgctgc acgaacgaat ctccttgccc gcctgggaaa
     1681 aggcctacct cgcggcacgc agcgtgacga aaagccgccg atgatcgata aaccgactct
     1741 cgtggtgatt ggcggcggcg ttgccgggtt gactgccgct gtcaccgcag ccgatgccgg
     1801 ctgggctgtc accgtgctcg aggcgcgcgc tcgactcggc ggcgcgacgc actcgttcac
     1861 ccgcgcgttc gatgacggcg aggtcatcgt cgacaacggc cagcatgtat tccttcggtg
     1921 ctgcaccgcc tatcgcgagt tcctgcaccg tctcggtgtt gagaacgaca cggtcatgca
     1981 gtcgcgtctc gacgtgcccg tcttcgaccc gacgacgggt cgtggtgcgc gtttgcgacg
     2041 cgaccgcctg cccgctccgc tgcatttgtc acgggcactg ctgcgctacc gtctgctcac
     2101 gccgatgcaa cgtgtgcgcg ccatcgttgc tgcactcatg ctgaatcgcg tcgaccgcaa
     2161 cgcaccgtcg tccgatgcgc aaacctttcg ttcctggctc aaggcacacg gccagtccga
     2221 cgcggcgatc gagcggttgt tcgacgtctt cacggtcgca accctcaatg cgcccgccgc
     2281 gcaggtctca ctttcccttg gtgccatggt ttttcaggac ggcttgctcc gtgaagccga
     2341 cgcgtgtgac atcggcatgg cgcgcgttcc gctcggtcaa ctgcatggtg acgccgcggc
     2401 cgcagcgctt agcgggcacg acatcaagat gcgcacgccg gttcgcgcgg tgacgcagct
     2461 gcccggcaat cgctggcagg tcgaaacaga cgatgcaagc atcgaggcgg acgccgtcgt
     2521 gctggcggtg ccccacgacg ttgcggccga cctgttgccg gcgggggcga tcgccgaccc
     2581 tgacgtcgtg cgcgggctcg acgtcgcacc gattctgaat gtgcacgtga ttttcgatcg
     2641 cgtcgtgctc accgaaccgt tcatcgccgc cgtcggatcc ccagcgcagt tcgtgttcga
     2701 ccgcaccgcg ccatcgggtc tgcgtgaagg caccggccag tatgtggcgg tgtcggtctc
     2761 ggctgccgac cagtggatcg acgctccggt ggcacgcctg cgcgaggtgt tcatgcctga
     2821 gctgcgcaag ctcctaccag ccgctcgcga cgcccacgtg ctcgacttct tcgtgacccg
     2881 cgaacgcgaa gcaacatttc gcccggtgcc gggcagtgcc gcgcgccggc tcgggccggc
     2941 gacagccctg ccgggcctcg tggtcgccgg tgcgtggacc gccaccggct ggcccgccac
     3001 catggagagc gcggttcgca gtggcttggc cgcgatcgat gagttgcaga agtcgcggcg
     3061 cacgtcaccg gcagactcgc gggacgtatc acaacaggcg tcggcatgac gaccgccgcg
     3121 gggcacccgc ccagcccccc gccgtccggc ctgcccgacc ctgacgtgca ggtcgtgctg
     3181 acgagatcgc gcgacctgac cgatccaccc atgcgcgagg cagtcggccg gctcgcgccc
     3241 gccatgcgcg acgtggccgc ctatcacctg ggttgggtcg acgccgatgg cagcgagagc
     3301 gagacccatc gcggcaaatc ggtccggccc gctctggcga tgttgtcggc agaggccgtc
     3361 ggcgcgccgc cagccgacag catccccggc ggactcgcgg tcgagctcgt gcacaacttc
     3421 tccctcctgc acgacgacgt gatggacggc gataccgagc gccgccaccg cccgaccgca
     3481 tgggcagtgt tcggcagctc cgcggcgatc ctcgccggcg attcgctgct atcgctcgcg
     3541 ttcgaggcac ttctcgacgt cgaaccggta cgcgcgcatc cggcccagcg ctcacttgcc
     3601 gccgccacgc agcggctgat cgccgggcag atcggcgatc tcgaattcga acgccgcctc
     3661 gacgtgaccc tcgacgaatg cctcgagatg gcagcaggca agacagctgc gttgatcgcg
     3721 tgttcggcgt cgatcggcgc gcacctcgga ggcgggccga caccggtcat ctcggcactc
     3781 gagggcttcg gttacgaact cggcctcgcg ttccagttgg tcgacgactt gctcggcatc
     3841 tggggctcgt ccgcaagtac cggcaagccc gtcggcgccg acctgcgcgc acgcaagaac
     3901 agcctgccgg tcgtg
//
LOCUS       DASWVF010000377         6066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_60552, whole genome shotgun sequence.
ACCESSION   DASWVF010000377 DASWVF010000000
VERSION     DASWVF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60552"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VGD55_06780"
     CDS             complement(<1..529)
                     /locus_tag="VGD55_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEY0870084.1"
                     /translation="MRHLSALTRAGVGAAGIVLSLAATANASATAPTPPGPTTTPVGS
                     APLVPASAQVEGAAPLTESISFEVVLRSRDQAGLNAFTTAVSTPGSPSYRQFLTPAQY
                     ANRFGPTAATISSVGSQLRALGLTVGTAQGSVVPVSGTLGQVSSAMHTSFRQYRLASG
                     RIAHANLTAPQLPTAV"
     gene            720..2927
                     /locus_tag="VGD55_06785"
     CDS             720..2927
                     /locus_tag="VGD55_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0870085.1"
                     /translation="MVDPEPDRAAPGAWRRVIADVIRFDPHGIRVRGGPRIAVGATIA
                     LAIGYANRSWAGGAAAAGGALSVGISSFVPSQRPRVALLISTAAAMALGTFVGSATSA
                     YAIPHVLAVAAFTFAGGLLVAVQPAATPVGLNAIVGLIVYGRFPGSVQTALQSAGLVA
                     AGGIFQVLLAVVMRGRSHVDRALAGLSGAYAELASYARDLDIARSGLPIAAAIDNAIV
                     DREFSFGVGVAGPAAQACTSLADEARRIRLELLSLASARAAAQESDRPDAALLATFER
                     LASTVEEFLAHVVDGLAHADVPEDLDEALAAAGDVIKELRHVAAAAPSSQLLAARAST
                     AGAALAGQLRAVAALLPDAVDVMQPAPWSAVRSAQAVRSAQRVSGKGARRAEAIAERM
                     AANLTWRSDGFQHAVRLALVVSAAAVLAHAVGIGRGYWLALTAVLVLRPEFSVTFTRG
                     VGRALGTLIGVGLATTIALTTHPHGWVLVALVGVFVWLTGALFNASYAVFSVFVTGAV
                     VFLLAGLDTDPVTTARDRLVATVLGSALALAAYALWPSWGRRPVADAVADLADATRRY
                     VSLALRAVMEPGTDSSKAAAASREVRLARTNAEAAINRSLGDPASRRIDHTLTLDVLA
                     TFRRLAIATHTLRLAPTAALDGVPSALRELVDALDSELGAIAARLRFGRVTVVRAPLR
                     AMHSDVTAALAGRAPLVEAGPLALLVIAETDEMVDATNSLAEILDRAPATVTT"
     gene            3258..5366
                     /locus_tag="VGD55_06790"
     CDS             3258..5366
                     /locus_tag="VGD55_06790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY0870086.1"
                     /translation="MKTAGAIAVTRTTGRQLPAGRRHLPGLDGLRAIAVVGVLLYHAG
                     VGWIPGGFLGVDLFFVISGFLITSLLLAESDLTGRISLRHFYLRRARRLLPALAAMLV
                     VVVSFMAVFFRGDLGQARGDVAAAASYVSNWWYVLHHRSYFVAAGRPSPLQHLWSLAV
                     EEQFYLVWPVVVVALVATRARLRWITVVAVAGALGSAFWMRTLAIRGNVPFDTDSSRV
                     YFGTDTHASALLLGAAAAAFMTGLAGRRVVTLPAIARHLIDMAALAGLVALGWSMYAV
                     GYYTPSLYRGGFLEFAAIAAVVVAIVSAPGGWLGRALDVAPMRWIGERSYGLYLWHWP
                     VFVYTRPGLDWQLHGAVALGVRLAIVAVLTELSYRFVEVPLRRRGVVSTVRAVRPMPR
                     SWRVVAPVLGAVLGVVGVVGATTFVSTRISQSSRASARHAAAAPGRTGGAEANGASGH
                     GGTLGPVSAVGAGSTARSGGAPVGALRPVASAAGSSPAVGRSTSASAGELAQSLPSSP
                     SPASRTQVAMPPTGAPTHPATAPTTARGYPTAPPPIPTGAPPALSAVGDSVMLDAAST
                     LKLVCPGTEVYAVVGWQAKAVFGELDTLRAAGHLGAVVVIETGTNGIVSPKELDAELT
                     ALADRAQVIVINDHMARPWEPSNNAMFPVVVKAHPNAVLVNWDDAANAHPDWLSKDGV
                     HLKPAGRGPYAQLIKTAAGC"
     gene            5410..5955
                     /locus_tag="VGD55_06795"
     CDS             5410..5955
                     /locus_tag="VGD55_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0870087.1"
                     /translation="MEYGLLMGIFRLNHAVLYVRNLDETVQFYTQVLGFQPIGMTPEG
                     FRGAAFLQAPGSTNDHDLGLFEIGPAAGPSGAGRTTVGLYHLAWEVDTLDELERVAEA
                     LTKADALVGSSDHGTTKSLYGRDPNGIEFEIVWLIPADLLDGDALEARRRIGHLDLEK
                     EKARYGATTQGGIGVSRVAVS"
BASE COUNT          865 a         2030 c         2086 g         1085 t
ORIGIN      
        1 ccacagcggt cggaagttgc ggtgccgtga ggttcgcgtg ggcgatgcga cctgacgcga
       61 gccggtactg ccgaaacgag gtgtgcatag cggaactgac ctggccgagt gtgccggata
      121 caggcacgac cgaaccctgt gcggtcccga cggtgagccc cagcgcgcgc aactgcgacc
      181 cgacgctgct gatcgtcgct gccgtggggc cgaaccggtt cgcgtactgg gctggggtca
      241 gaaactgccg atacgacggc gagccgggtg tcgacaccgc tgtggtgaac gcgttgagac
      301 cggcctggtc gcgcgaccgc agcaccacct cgaacgatat ggactcggtg agcggtgcag
      361 cgccctcgac ctgtgcgctc gcggggacca gcggcgcgga cccgacagga gtggttgtgg
      421 ggccgggcgg cgtgggcgct gtcgccgaag cgttggctgt cgcggcgagg ctgagaacga
      481 tcccggcggc gcctacaccc gcgcgcgtaa gtgcacttag gtggcgcacg aatctccccc
      541 agctcggctg actgtgacgc gcccgctccg ccaccttgcc tcccacgata gtgcggcgac
      601 ggcgtcgaac gggcgagatg ggcggttgtg tctgtcgcac cacccatttg cgccggaccg
      661 taggctgcct ccagtcggcg ggtgcagggc aggtgacggg ccgagtggag gcgccgacca
      721 tggtcgaccc ggagcccgat cgcgccgccc cgggcgcatg gcgacgagtc atcgccgacg
      781 tgatccgctt cgacccacac ggcatcaggg tgcggggcgg cccgcggatc gcggtcggcg
      841 ccacgatcgc tcttgccatc ggctacgcga acagatcatg ggccggcgga gctgcggcgg
      901 ccggtggtgc gctctcggtg ggcatctcat cctttgttcc atcgcaacgt ccgcgcgttg
      961 cgctgctcat ctcgacggcc gccgcgatgg cgctcggaac attcgtcggt tcggccacta
     1021 gtgcctacgc gatcccgcac gtgctcgcgg tcgctgcctt caccttcgca ggcgggctgc
     1081 tcgtcgccgt ccagcctgct gcgacgcccg tcggattgaa cgcgatcgtc ggcctgatcg
     1141 tctacggacg ctttccggga tctgtgcaga cagcgttgca aagcgcgggc ttggttgctg
     1201 ccggcggaat ctttcaagtc ttgctcgccg tggtgatgcg cggtcgttcg cacgtcgacc
     1261 gcgcactcgc cggactctcg ggcgcctacg cagaactcgc gtcgtacgcc cgagacctcg
     1321 acatcgcacg ttcaggtctg ccgatcgccg cagccatcga caacgcgatc gtcgaccggg
     1381 agttctcgtt cggggtcgga gtcgccggcc cggcggcgca ggcgtgcacg agtctcgccg
     1441 acgaagctcg tcgcatcagg ctcgagttgc tgagcctcgc gagtgcgcgt gcagctgcgc
     1501 aagaaagcga tcggccggac gccgcgttgc tggcaacttt cgaacggctg gcatcgaccg
     1561 tcgaagagtt tctcgcccac gtggtcgacg gtcttgcgca tgccgacgta cctgaggatc
     1621 tcgacgaggc gctcgccgcc gccggcgatg tcatcaagga actgcgtcac gtggctgccg
     1681 cggcgccctc ctcgcaactg ctcgcggcac gggcaagtac cgcgggcgcc gccctcgctg
     1741 gacagttgcg cgccgtggcc gccttgctac ccgacgccgt cgatgtgatg cagcccgcac
     1801 cttggtcggc ggttcgctcg gcgcaagccg ttcggtcggc acagcgggtg tcgggcaagg
     1861 gggcgcggcg ggccgaggca atcgctgagc ggatggcagc gaatctgacc tggcggtccg
     1921 acgggttcca gcacgctgtt cgactcgcgc tggtcgtcag tgcggcggcc gttcttgctc
     1981 acgcggtggg catcggccgc ggttattggc tcgcgcttac ggccgtcctc gtgttgcggc
     2041 cggaattctc ggtgacgttc acccgagggg tcggtcgggc gctcggcacg ttgatcgggg
     2101 tcgggctcgc aacaacgatc gcgctgacga cgcacccaca cggctgggtg ctggtcgcgt
     2161 tggtcggcgt gttcgtctgg ctcacgggcg cgttgttcaa cgcgagttac gcggtgttca
     2221 gcgtgttcgt caccggagcc gtcgtgttct tgctggccgg tctcgacacc gatcccgtca
     2281 caaccgcgcg cgatcgcctc gtcgccaccg tgctcgggtc ggcgctcgcc ttggccgcgt
     2341 acgcactgtg gccgagttgg ggtcggcgcc cggtcgccga tgcggtcgcc gatctcgccg
     2401 atgcgactcg ccgctacgtc tccctggcct tgcgcgcggt gatggagccg ggcaccgatt
     2461 cgtcgaaggc ggctgccgcc agtagggagg tcaggctcgc ccgcacgaac gccgaggcgg
     2521 cgatcaaccg atcgctcggc gacccggcat ctcgccgcat cgatcacacc ttgactctcg
     2581 acgtcctggc gacctttagg cggcttgcca ttgccaccca cacgctgcgc ctggctccga
     2641 cggcggcgct ggacggcgtc ccctctgctt tgcgtgagct cgtcgacgcc ctcgactccg
     2701 aattgggggc gatcgcggcg cggttgcggt ttggccgggt cacggtcgtg cgggcaccac
     2761 tgcgcgcgat gcacagcgac gtgacggcgg cgctggcagg acgcgcgccg ctcgtcgaag
     2821 ccgggccgct cgcgctcctg gtgatcgccg agaccgacga gatggtcgac gccacgaact
     2881 cactcgccga gatcctcgac cgcgcgcccg ccaccgtcac aacctgagcc cgacccgccg
     2941 tcccttcatc gagtgtgcgc aatcggctct gaattcgctg tcggacgagc cgattgtgaa
     3001 cagtcggtga cacgcaaagt ggggcggacg cggaggcaac cgattgcggg cagcgcgcgt
     3061 cgtcttggcg tggcactcat cggacacgtc gcgccgaccg tgacgcgatc tgggatcggt
     3121 tgcggcgcgc cggtcaagaa cgagcggagc ggctccgatg caatgtcaca ctcgggaacg
     3181 gttgcggtct tcttcccatc gaagacctca accctctcca cctcgaagat ctcaaaaaga
     3241 atgtggcagg ggttggcgtg aagactgcgg gggccatcgc ggtcacccgg acgaccgggc
     3301 gccaacttcc tgcgggccgt cgccacctgc cggggctcga cggattgcgc gctatcgcgg
     3361 tcgtcggggt gttgttgtac cacgctggcg tcggctggat cccgggcggc tttctcggcg
     3421 tcgacctgtt cttcgtgatc tccggcttcc tgatcacatc attgctgctc gccgagtctg
     3481 acctgaccgg ccgcatctcg ctgcggcact tctacctgcg ccgcgcgcgc cggttgttgc
     3541 ccgcgctcgc ggccatgctc gtggtcgtcg tttcgttcat ggcggtgttc ttccgcggtg
     3601 acctcggaca agcgcgcggc gacgtcgcag cggcggcgag ctacgtctct aactggtggt
     3661 acgtgctgca tcaccggtcg tacttcgtcg ccgccgggcg cccgtcgcca ctgcaacatc
     3721 tgtggtcgct tgccgttgag gaacagttct atttggtgtg gccggtcgta gtcgtcgcgt
     3781 tggttgccac gcgcgcgcgg ttgcggtgga tcaccgtcgt tgccgtcgct ggtgcactcg
     3841 gatccgcgtt ctggatgcgc actctcgcga tccgcggcaa tgtgccgttc gacaccgact
     3901 cgtcgcgcgt ctacttcggc accgatacgc acgcgagtgc attattgctc ggcgctgccg
     3961 ctgccgcttt catgacgggt ctagccggac gtcgagtggt gaccctgcct gcaatcgcgc
     4021 gccatctcat cgacatggcg gcactcgcgg gtctcgttgc gttgggctgg tcgatgtacg
     4081 cggtcggcta ttacacgccg tctttgtatc gcggtggatt cctcgaattc gcagcaatag
     4141 cagcagttgt cgtggcaatc gtgtcggcac ctggtggctg gctcggtcgt gcactcgacg
     4201 tcgctccgat gcgctggata ggtgagcggt cgtacggcct ctatttgtgg cactggccgg
     4261 tgttcgtcta caccaggccc gggctcgatt ggcaattaca cggggcggtc gccctcggcg
     4321 tccggctcgc gattgtcgcg gtgctgaccg agctgagcta ccggttcgtc gaggtcccgt
     4381 tgcggcgacg cggtgttgtg tcgacggtgc gtgccgtaag accgatgccg cgatcgtggc
     4441 gggtcgtggc gccggtgctt ggcgcggtgc ttggtgtcgt cggtgttgtc ggcgcgacga
     4501 cattcgtgtc gacgcgcatc agccagtcca gtcgagcaag cgcacggcac gccgccgccg
     4561 cgcccggacg tacaggcggc gccgaagcta acggcgcgtc gggtcacggc ggcactcttg
     4621 gcccggtcag cgctgtcggt gctggcagca ctgcccgctc tggcggcgcg ccggtcggtg
     4681 cgcttcggcc ggtcgcgtcc gcggccggtt cgtcgcccgc tgtgggccgc tcaacgtcag
     4741 catccgcggg cgaactggcc cagtcattgc cgtcatcgcc atcaccggcc agccgcacgc
     4801 aggtcgcgat gccgccgacc ggcgcaccca cccatcccgc aactgcaccg acgaccgcca
     4861 ggggatatcc gacggcgccg cccccgattc ccaccggcgc acctcccgcg ctcagtgctg
     4921 ttggtgactc ggtgatgctc gacgcggcct cgacgttgaa gctggtgtgc cccggcaccg
     4981 aggtgtacgc cgtcgtcgga tggcaggcca aggcggtgtt cggcgaactc gacacgctgc
     5041 gagcggccgg ccacctcgga gccgtcgtcg ttatcgagac gggaaccaac ggcatcgtca
     5101 gcccgaagga actcgacgcc gaactcaccg cgctcgctga ccgggcacag gtgatcgtta
     5161 tcaacgacca catggccaga ccgtgggagc cgtcgaacaa cgcgatgttc cctgtcgtgg
     5221 tgaaggcgca ccctaacgcc gtgctggtga actgggacga cgcggcgaac gcgcacccgg
     5281 actggctcag caaggacggc gtgcacctga agccagccgg tcggggaccg tacgcccagt
     5341 tgatcaagac cgccgcgggg tgctgagatt cagagcaaac gatcataggt agttgaacgc
     5401 tcaactataa tggagtatgg tctgctcatg ggaatcttcc ggcttaacca cgcagtgctt
     5461 tatgtgcgca atctcgatga gaccgtgcag ttctacacac aggtccttgg gttccaaccg
     5521 atcggcatga cgcccgaggg cttccgtggc gcggcgttcc tccaggcgcc cggatcgacg
     5581 aacgaccacg acctcggcct cttcgagatc ggtcccgcag cgggtccttc gggcgccggg
     5641 cgcacgacag tcggtctgta ccaccttgcg tgggaggtcg acacactcga cgaactcgag
     5701 cgagtcgctg aggcgctgac gaaagccgac gccctcgtcg gtagctccga ccacggcacc
     5761 accaagagcc tgtacgggcg cgacccgaac ggcatcgagt tcgagatcgt ctggctcatc
     5821 ccggccgacc tgctcgacgg cgacgccctc gaggcgcgcc ggcgcatcgg tcatctcgac
     5881 ctcgagaagg agaaggcgcg ctacggcgcg acgacgcagg ggggcatcgg cgtatcgcgg
     5941 gtcgctgtca gttgaccggt tgcgttccgg cacgatggcg cccatgagta gcgacgcagc
     6001 agcgacgagc gaccaccggc cagccgaaca attccgggtg gccatcgtcg gctacgggct
     6061 ggccgg
//
LOCUS       DASWVF010000378         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_35441, whole genome shotgun sequence.
ACCESSION   DASWVF010000378 DASWVF010000000
VERSION     DASWVF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35441"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGD55_06800"
     CDS             <1..738
                     /locus_tag="VGD55_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0870088.1"
                     /translation="TGQNPGSLLDGLHSVQVVSDAAAASLSHVDSSVQAAADADAKVA
                     ALRTQQDALTQQAANASTDAQSALAQEQQILDNTNTEVVALEAQLQIQIDEQNAARDA
                     ATLAAAQQAALVAGGAAGAPSALAQTAIAAARTQLGKPYVYGGSGPDSWDCSGLTQWA
                     FAQAGVLLPRTAADQYAAVPTKVQLGNLEPGDLLFWATDTSDPSTIHHVAIYLGAGEM
                     LAAPHTGDLVQIQPVYLDGYIGAVRVG"
     gene            831..2354
                     /locus_tag="VGD55_06805"
     CDS             831..2354
                     /locus_tag="VGD55_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEY0870089.1"
                     /translation="MAPGLLIAERYRLESPLSAETTSQRVGRLWRAYDEVLARPVAIL
                     LVEGDDPGVPEVLAGARAAARLTHAAVVRVYDAGEAPGLAFVVTEFLSGGSLEAQLLV
                     GPLDPSNAVDLVSDLAVGVAAAHDVGLHGLSLTPSSVLFTATGAPRLVGIALADHNMR
                     DATVNGPDGHPLAPHADKVEQARLDAIGLARLLYAALTAKWPGPPPESALPPAPYAEG
                     HLRTPRQVRGGVAREIDAVVAEAIGDESLLRGLAPITSPAEFAAALAPLRTSSDDAHP
                     YGADTQPITELDLRQVRRSAGRIAGEGRWRRIAIVIGVALLGVVIAGLAFSGSSGYLR
                     FVTGAPEPGPTATPTPTPTTTTAAVNTTTTGLVTEFDPYGNHKDPHVAEAPRAVDGNP
                     ATAWRTQIYRTADLGKLKPGVGLLVDFGSPRKVSAVHLVLVGNGTSVELFESTGDTAP
                     TSEKAMTSVASQANAPADVTLRPDDATSARFWMIWLTTLPATAAGFQGGIAEMTFLP"
     gene            2386..3000
                     /gene="sigM"
                     /locus_tag="VGD55_06810"
     CDS             2386..3000
                     /gene="sigM"
                     /locus_tag="VGD55_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HEY0870090.1"
                     /translation="MTDGVADPDVELLTRHLAGDPDAFTEIVHRHRDQLWAVALRTTG
                     DPEEAADAVQEALISAMRHAASFRGDAKLSTWLHRIVVNACLDRLRRRASHPTSALPD
                     DDHAPTNSRDEFADRDTAADVHAALATLPVEQRAAIVLVDIEGYPVDEVAMMLEVPTG
                     TVKSRCARGRAKLVPLLSHLRTGGNPGDADRVEPPMNPPREDFR"
     gene            2997..4034
                     /locus_tag="VGD55_06815"
     CDS             2997..4034
                     /locus_tag="VGD55_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870091.1"
                     /translation="MNTPHPDLETLAELADPEAATAAAAADPETAAVLDHVASCETCT
                     ANLEALREVRAKLRALPPVQMPADVAKRLDAALRAANAPTANVVSLQKRSKQRAGESY
                     QPRWRTMPYGAAASVVVILALVVAGVVGLQHAGGGSKKSSASAAASSAPAAATAGSAT
                     VESGTNYTADQIRAQVAVVVNSNVPGAAAQFPGLSQFGADASSAAPAVAAGAAAPAAS
                     ASAAAAAPAVSVPAAAATTAAAPASSPNFAAAATGGALAPQRNAAAITAPGGPLADPA
                     TLAACIQILAGKPELPVLVDFATFNGAPSTIIVLPDPDVARKLDIYVEADTANCANQE
                     FTYAAFLPPPG"
     gene            4144..>4520
                     /locus_tag="VGD55_06820"
     CDS             4144..>4520
                     /locus_tag="VGD55_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0870092.1"
                     /translation="MVTTSTNAMAGNDATAVRDLIIIGSGPAGYTAALYAARADLRPL
                     VFEGAIGAGGALMTTTEVENFPGFPGGIMGPDLMDNLRKQAARFGAQLVTEDVTAVDL
                     TGPVKVVQVDDQTYYSRSVILATG"
BASE COUNT          773 a         1612 c         1447 g          688 t
ORIGIN      
        1 accggccaaa accccggctc gttgctcgac ggtctgcaca gcgtgcaagt cgtctccgac
       61 gcggcagcag catcgctttc tcacgtcgat tcgtccgtgc aagcagcggc ggacgccgac
      121 gccaaggtcg ccgcgctacg aacccagcaa gacgcgctga cccagcaagc agcaaacgca
      181 tcgactgatg ctcagagcgc gttggctcag gagcaacaga tcctcgacaa caccaacacc
      241 gaggtcgtgg cgctggaagc tcagctgcaa atccaaatcg acgagcagaa cgcggccaga
      301 gacgcagcaa cgctggccgc cgcccagcaa gctgcgttgg tcgcgggcgg tgcagcgggc
      361 gcgccaagtg cgctggctca gactgcgatc gccgccgccc gcacccagct aggaaagccc
      421 tatgtatacg gcggttcagg tcccgacagc tgggattgct ccgggcttac gcagtgggcg
      481 ttcgcgcaag ccggcgtcct ccttccacgt acggcggctg accaatacgc agcagttccg
      541 acgaaggtgc agctcggtaa cctcgaacca ggggacctcc tgttctgggc gaccgatacc
      601 tcggatccgt cgacgattca ccacgttgcg atctacctgg gtgccggcga gatgctcgcc
      661 gctccgcaca cgggcgacct cgttcagatt cagccggtct acctcgacgg ctacatcgga
      721 gccgttcggg tcggctgacg ggaatagcat gggatctcga ttgcgcgcag tatcccgagc
      781 acccgggcag cgagcaccgc gggaggtctg ctgtgggcgg cgcaccggcc gtggcacctg
      841 gcttgctcat cgccgagcgt taccgcctcg aatccccgct atcagctgag acaacatccc
      901 agcgagtcgg ccgcctgtgg cgggcttacg acgaagtact cgcccgaccg gtggcgatcc
      961 tgcttgttga gggcgacgac ccgggcgtac cggaggttct cgccggtgcg cgagcagccg
     1021 cgcgattgac gcacgctgcc gtcgtccggg tctatgacgc aggagaggcg cccgggctgg
     1081 ccttcgtcgt cacggagttt ctgtcgggag gctcgctcga ggctcagctg ctggtcggcc
     1141 cgctcgatcc gtcaaatgca gtcgatctcg tctccgacct cgcggtcggg gtggcagccg
     1201 ctcacgacgt tgggctgcac ggtctttcgc tgacaccgag cagcgtcttg ttcaccgcaa
     1261 ccggcgcgcc acggctcgtc ggcatcgcgc tggccgatca caacatgcgc gacgccaccg
     1321 tcaacgggcc tgacggtcac ccgctcgccc cgcacgccga caaggtcgaa caggcacgtc
     1381 tcgacgcgat cggcttggca aggcttctct acgcggcctt gaccgcgaag tggccaggac
     1441 caccacccga aagcgcgttg cctccggcgc cttacgccga gggccacctg cgcacgccgc
     1501 gtcaggtccg gggtggggtg gcgcgggaaa tcgacgcagt cgtcgctgaa gcgatcggcg
     1561 acgagtcctt gctccgcgga ctggccccga tcacctcacc cgccgagttc gctgcggccc
     1621 tggcgccgct gcgcacatcg agcgatgacg ctcatcccta cggcgccgac acgcaaccga
     1681 tcaccgagct cgaccttcgg caagtccgtc gcagtgcggg ccgaatcgcc ggtgaagggc
     1741 gatggcgccg catcgcgatc gtgatcgggg tcgctctgct cggagtcgtc attgcgggcc
     1801 tggcattttc cggctcgtcg ggttacctgc gattcgtcac gggagcacca gagcccgggc
     1861 ctacggcgac gccaacccct acaccgacaa ccacgaccgc cgccgtgaac acgaccacga
     1921 ccggcctcgt taccgagttc gacccgtatg gcaatcacaa ggacccgcac gtcgccgagg
     1981 cgccgcgcgc cgttgacggc aacccggcga ctgcgtggcg aacacagatc taccgcacgg
     2041 cagacctcgg caagctcaaa cctggcgtcg ggctgctcgt cgacttcggc tcgccccgca
     2101 aggtttccgc cgtccacctt gttcttgtgg gcaacgggac ctccgtcgaa ctgttcgaga
     2161 gcacgggcga caccgcgcct acgagtgaga aggcgatgac gtccgtggcg tcgcaggcca
     2221 acgctccggc cgacgtcacg ttgcgacccg acgacgcgac gtccgcgcgg ttttggatga
     2281 tctggctgac cacgttgccc gcgacggctg cgggcttcca gggcggcatc gcagagatga
     2341 ccttcctgcc gtgatttggc ccgtcacgtc gataggctgc cgggcgtgac ggacggggtg
     2401 gcagatcctg acgtcgagtt gctgactcgg catctcgccg gagaccccga tgcgttcacc
     2461 gagatcgtgc accggcaccg cgaccagctg tgggcggtcg ccttgcgaac gaccggtgac
     2521 cccgaagagg ccgccgacgc agtgcaagaa gcgctgatat ccgcgatgcg ccatgccgcc
     2581 tcgtttcgcg gcgacgcgaa actaagcacc tggttacacc gcatcgtggt caatgcatgc
     2641 ctggatcgat tgcgccgccg cgcctcgcat ccgacaagcg cgctgcccga cgacgaccac
     2701 gcacccacca acagccgcga cgagttcgcc gaccgcgata ccgctgccga cgtgcacgcc
     2761 gcgctggcga ccttgccagt cgagcaacgg gcagcaatcg tccttgtcga tatcgagggc
     2821 tatcccgtcg acgaggtcgc aatgatgctc gaagtgccta cgggcacggt caagagccgg
     2881 tgcgctcgcg gtcgcgcgaa gctggtcccc ctcctctcgc atcttcggac cggagggaac
     2941 ccgggcgacg ccgatcgcgt cgaaccaccg atgaacccac cccgagagga tttccggtga
     3001 acaccccgca cccggacctc gagacactcg cggaactcgc agaccctgag gcggcaaccg
     3061 cagctgcagc agccgacccc gagacggcgg ccgtactcga tcatgtcgcc agctgcgaga
     3121 cgtgcacggc caacctcgaa gctctgcgtg aggtccgggc gaagttgcgc gcgctcccgc
     3181 cggtgcagat gccagcagat gtcgcgaagc ggctcgacgc cgcactgcgg gcagcgaacg
     3241 ccccgaccgc caacgtcgtt tcgctccaga agcgctcgaa gcagcgcgcg ggcgagtcat
     3301 accagccgcg gtggcggacc atgccgtacg gcgccgcggc ctcggtcgtc gtgatcctgg
     3361 cgctcgtcgt agcgggcgtt gtgggtttgc agcacgctgg cggtggctca aagaagagct
     3421 cggcaagtgc cgctgcctcc tccgcgccag ccgcagcgac tgcgggctca gcaacggtcg
     3481 agtccggcac gaactacaca gccgatcaaa tacgcgccca ggtcgcggtg gtcgtgaaca
     3541 gcaacgtgcc gggagcggcc gcgcagttcc ctggcctgag ccagttcggc gccgacgcga
     3601 gctcagccgc acctgctgtt gcggcaggtg cagcggcgcc tgccgcgtct gcgtcagcgg
     3661 ccgccgctgc gccagcggtt tccgtgcctg cagctgcagc tacgacggcc gccgcgcccg
     3721 ccagctcccc caatttcgcc gccgcagcta ccggcggagc cctggcacct cagcgaaacg
     3781 ccgccgcgat cactgcgccc ggcggaccgc ttgccgaccc ggccaccctt gcggcgtgca
     3841 tccagatcct tgctggcaag ccggagctgc ccgtgctggt cgacttcgcc actttcaacg
     3901 gcgcgccgtc gacgatcatc gtgctgccgg accccgacgt cgcacggaag ctcgacatct
     3961 acgtcgaggc cgacaccgcc aattgcgcga accaggaatt cacctacgcg gccttcctcc
     4021 caccacccgg gtgaccgagc acgcgatccg gccgagccgg gaatcgcggc agcctaggat
     4081 ctgttgacgg gtaagccgcc gaagctggcg agcaacttcg agagaggtcc aaggtaggtc
     4141 gacgtggtca caacaagcac gaatgcgatg gccggcaacg acgccaccgc cgtgcgcgac
     4201 ctcatcatca tcggttcagg cccggcgggt tacaccgcgg cgctctacgc ggcgcgcgct
     4261 gacctgcgtc cgctcgtgtt cgagggcgcg atcggcgccg gcggtgcctt gatgaccacc
     4321 accgaggtcg agaacttccc cggattcccg ggcggcatca tgggccccga tctcatggac
     4381 aacctgcgca aacaagcggc tcgcttcggc gcccagctcg tcaccgaaga tgtcaccgct
     4441 gtcgatctga ccggcccggt gaaggtcgtc caggtcgacg accagaccta ctactcccgc
     4501 agcgtgatcc tcgcgaccgg
//
LOCUS       DASWVF010000379         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_494068, whole genome shotgun sequence.
ACCESSION   DASWVF010000379 DASWVF010000000
VERSION     DASWVF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494068"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGD55_06825"
     CDS             complement(<1..218)
                     /locus_tag="VGD55_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870093.1"
                     /translation="MTTDAMAPGEAENRARDLSLERTSIRGAYWTGSRLWVALSAMLF
                     GSAVFTYFYLHSLDNDTLWRAPGQRQSG"
     gene            complement(220..2187)
                     /locus_tag="VGD55_06830"
     CDS             complement(220..2187)
                     /locus_tag="VGD55_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0870094.1"
                     /translation="MAMDTTAPPAVDRAGNPGGPPAPRATRPWWLSPNLVLAFIGAAA
                     GYAIGHMVGNWIAHTYQQVQGEGQNNHAIVLGYLFATFGWLAGIGALNYPVQKLLGYA
                     PASEAEGAHERKPTLRSYFSYTLDHKVVGVQYLVAMLFYFFVAGLFALAIRSELLSPT
                     RHIFQPGTYIEIVGEHGTMMMMLMTSVILGPFGNYLVPLMIGSRRMAYPRLEALSFWI
                     TPAAFIVLLSGIIVGGFPTGWTGYAPLSIEAGEGQDAYLVAFGLMALSMIVGGFNIIC
                     TIINYRAPGMTWTRVPVFVWSMLVTAMLMVLAAPVLVAATYMGLMDRTVQTALFDAQH
                     GGSSYLWENLFWFFGHPEVYILALPGFGIALELLAVFARKPVFGYRTAVAGMVGVGVL
                     SFFVWQHHLFVSGMNPDMRPVVMLTTELISIPTGIIYLAAVGTLWRARLRLTVPILFV
                     LGMLFNFLIGGISGVFLSDVPTDAVAHGSFFVMAHFHYTIMGGLIFTFFGGVYYWLPK
                     MTGRQLNQKLGKIHFWTLFIFFNSTFLPLFAIGELGMPRRVFEYEKALQGLNDWVSFS
                     AYLIGLSMLIFVYNFVKTMFIDPLPTEPVGNPWGSRGLEWMTSTPPVAYNFAHVPTVV
                     SSPYEYGTGAGPMAYIGGEPPGVPTQHEGVN"
     gene            complement(2201..3157)
                     /locus_tag="VGD55_06835"
     CDS             complement(2201..3157)
                     /locus_tag="VGD55_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0870095.1"
                     /translation="MTSASSAPRTDRQHIMRIAVIWVVLSVIGMVLVYFVWGPHMPPG
                     KDSDQARVQQDGNRLLGTIAVPVFVFVWVYLGYVLTTWRVKSSTPVDEVGDGPPLKGH
                     AGFQAIWLGVTTATVLGLFVFGTVDLAHNDGAGTGSGPSPIWTPTGYDQNAAQSKLLV
                     VQVIGQQWRWTFRYPQYGGLESAEMVVPAGQEVQYSVTSLDVIHSFWAVNLGIKADAN
                     PGVNNVAFGTADKIGDINIRCDELCGLYHGSMTSTGKVVSAADFKTWVTNASATYQQL
                     IPFLPPYANTYLPQSDGGYYDPGQDPLPSGPPPGPASPSVIP"
     gene            complement(3157..3822)
                     /locus_tag="VGD55_06840"
     CDS             complement(3157..3822)
                     /locus_tag="VGD55_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870096.1"
                     /translation="MSRSIRTTVVGVVFAVFVAGVVMLFLYFLHYPPTVAAAATGADE
                     EIHLQAVPALGYGPHPTYVSYLADINGKWVHTTMFKLKANTLVHFTIDQYDTGSLLRN
                     SFMDQVQGTVGGTEVLNGGAPVRIINDNTDNGIGHTFAVPSLGISVPMPGIASAATNV
                     CSIAPCDSKFDHNTITFTIMTPAAGLYPWQCFVPCGLGWPYGNGGGMSTLGYMNGFLK
                     VVA"
BASE COUNT          794 a         1223 c         1317 g          662 t
ORIGIN      
        1 aacccggact gtcgctgccc cggcgcccgc cacaacgtgt cgttgtcaag ggagtgcagg
       61 tagaagtagg tgaacacggc cgatccgaag agcatcgctg acagcgccac ccacagccgc
      121 gagcctgtcc agtaagcccc gcgaatcgac gtccgctcaa gactgagatc gcgcgcgcga
      181 ttctctgcct cacctggtgc catcgcatcg gtcgtcacgt cagttcaccc cttcgtgctg
      241 agtcgggacg ccgggtggct cgccgccgat ataagccatc ggtccggcgc cggtgccgta
      301 ttcgtacggg ctcgacacga ccgttggcac gtgcgcgaag ttgtacgcga ccggcggtgt
      361 ggaggtcatc cactccaggc cgcgcgaacc ccacgggttc ccgaccggct cggtcgggag
      421 cgggtcgatg aacatcgtct tcacgaagtt gtagacgaag atcagcatcg acaggccgat
      481 caggtaggcc gagaacgaca cccagtcgtt gaggccttgc aacgccttct cgtattcgaa
      541 gacgcggcgc ggcatgccca gctcgccgat ggcgaacagc ggcaggaacg tcgagttgaa
      601 gaagatgaac aacgtccaga agtgaatctt gccgagcttt tggttgagct ggcgaccggt
      661 catcttcggc agccagtagt agacgccgcc gaagaacgtg aagatcagcc cacccatgat
      721 cgtgtagtgg aagtgggcca tcacgaagaa gctgccgtgt gcaacagcat cagtcgggac
      781 gtcggaaagg aacacgcccg agatgccgcc gatgagaaag ttgaagagca tgccaagcac
      841 gaacaagatc ggcacggtga gtcgtaatcg cgcgcgccac agggttccga cagcggccag
      901 gtagatgatg cccgtcggga tcgaaatcag ttcggtggtc agcatgacga ccggccgcat
      961 gtcggggttc atgccgctga cgaacaggtg gtgctgccag acgaagaacg acagcacacc
     1021 gacgccgacc atgcctgcga ccgccgtgcg gtaaccgaac accggcttgc gtgcgaagac
     1081 cgcgagcaac tcaagcgcga tgccgaaacc gggcagcgcg aggatgtaca cctcggggtg
     1141 gccgaagaac cagaacaggt tctcccacag gtatgagctg ccgccgtgtt gcgcgtcgaa
     1201 caaggcggtc tggaccgttc gatccatcaa ccccatgtac gtcgcggcga ccagcaccgg
     1261 cgcggcgagc accatgagca tcgctgtcac gagcatcgac cagacgaaga ccggcactct
     1321 cgtccaggtc ataccaggcg cccggtagtt gatgatcgtg cagatgatgt tgaagccgcc
     1381 gacgatcatc gagagcgcca tcagcccgaa cgcgacgaga taggcgtctt ggccttcacc
     1441 ggcttcgatc gatagcggcg cgtagccggt ccagccggtc ggaaaaccgc cgacgatgat
     1501 gcccgacagc aagacgatga acgcggccgg cgtgatccag aacgagagcg cctcgaggcg
     1561 cgggtaggcc atgcggcgcg agccgatcat cagcggcacg aggtagttgc cgaacgggcc
     1621 gaggatgacc gacgtcatca acatcatcat catcgtgccg tgctcgccga cgatctcgat
     1681 ataggtgccg ggctggaaga tgtggcgcgt cggtgacaag agttcagagc ggatcgccaa
     1741 cgcaaacaag ccggcgacga agaagtagaa caacatcgcg acgaggtact gcacgccgac
     1801 gaccttgtgg tcgagcgtgt agctgaagta cgaccgcagc gttggcttgc gctcgtgcgc
     1861 cccttcggcc tcgctggccg gcgcgtagcc gagcagcttc tggaccggat agttcagcgc
     1921 accgataccg gcgagccagc cgaaggtggc gaacaagtag ccgagaacga ttgcgtgatt
     1981 gttctgacct tcaccctgca cctgctggta ggtgtgcgcg atccagttgc cgaccatgtg
     2041 cccgatcgcg tatccggcag ctgcgccgat gaatgcaaga accaggttcg ggctcagcca
     2101 ccagggccgc gtcgcgcgcg gtgctggtgg tccgccggga ttgccggccc tgtcgaccgc
     2161 tgggggcgcc gtcgtatcca ttgccatgag tcgcggcctc ctacggaatc acgctgggcg
     2221 aggcgggacc aggcggcggt ccgctgggca gcggatcctg gcctgggtcg tagtacccgc
     2281 cgtccgactg cggcaggtaa gtgttcgcat acggcggcag gaagggaatc aactgctgat
     2341 acgtcgctga cgcgttcgtg acccacgtct tgaagtcggc ggcgctcacg accttgcccg
     2401 tcgacgtcat cgagccgtga tagaggccac acagctcgtc gcatcggata ttgatgtcgc
     2461 cgatcttgtc ggcggtgccg aacgcgacgt tgttcactcc agggttggcg tcggccttga
     2521 tgccgaggtt cacggcccag aacgaatgga tgacgtcaag ggaggtgacg ctgtactgaa
     2581 cctcctggcc cgcgggcaca accatctcgg cgctctcgag gccgccgtat tgcgggtagc
     2641 ggaaggtcca acgccactgt tgcccgatga cctggacgac gagcagtttg ctctgagcgg
     2701 cgttctggtc atagccggtg ggcgtccaga tcggtgacgg ccccgagccc gtaccggcgc
     2761 cgtcgttgtg ggccaggtcg actgtgccga acacgaagag cccgagcact gtcgccgtcg
     2821 tgacgccaag ccagatcgcc tggaacccgg cgtgcccctt cagcggcgga ccgtcgccga
     2881 cttcgtcgac gggcgtgctc gacttgacgc gccaagtggt cagcacgtag ccgaggtaga
     2941 cccatacgaa cacgaagacg gggacggcga tcgtgccgag cagccggtta ccgtcttgct
     3001 gcacacgcgc ctggtcggag tctttgcccg gcggcatgtg cgggccccac acgaaataga
     3061 ccagcaccat gccgatgacc gacagcacga cccagatcac agcaatgcgc atgatgtgct
     3121 gccggtcggt gcgtggtgca gaggacgcag atgtcattag gccacgacct tcaggaagcc
     3181 gttcatgtag cccagcgttg acatcccgcc gccgtttccg tagggccagc caaggccaca
     3241 ggggacgaag cattgccagg ggtaaagccc ggccgccggc gtcatgatcg tgaacgtgat
     3301 cgtgttgtgg tcgaacttcg agtcacaggg cgcgatgctg cacacgttgg tggcggccga
     3361 cgcgatgcct ggcattggta ccgagatacc gagcgacggg acggcgaacg tgtgcccgat
     3421 tccgttgtcg gtgttgtcgt tgatgatgcg caccggcgcg ccgccgttga gaacctcggt
     3481 gccgccgacg gttccttgca cctggtccat gaaggagttg cgcaggaggc tgccggtgtc
     3541 gtactggtca atggtgaagt gcacgagggt gttcgccttc agcttgaaca tcgtcgtgtg
     3601 cacccacttg ccgttgatgt cggcgaggta cgacacatag gtcgggtgcg gtccgtagcc
     3661 gagcgccgga acggcctgga gatggatctc ttcgtccgcc ccggtcgctg ccgcggcgac
     3721 cgtgggtggg tagtgcagga agtacaagaa caacatgacc acccccgcca cgaagacggc
     3781 gaacacgacc ccaacgacgg tcgtgcgtat ggatctgctc atctcggccc tcctgtcggc
     3841 gggttcgtca taaccatgac cgcaccaaga cgtcgagtga cgcggcgata cacagcaagc
     3901 tcaagtaggt gatcgaccag tggaacagcc gcatcggctg cgggtcgtct ccccgggcga
     3961 cgcgcgagcg cagcgcgtgg gcctcacgga ggaaca
//
LOCUS       DASWVF010000380         5994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_60738, whole genome shotgun sequence.
ACCESSION   DASWVF010000380 DASWVF010000000
VERSION     DASWVF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60738"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..2489
                     /locus_tag="VGD55_06845"
     CDS             342..2489
                     /locus_tag="VGD55_06845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY0870097.1"
                     /translation="MVVQLSGVDRDRHEVSVPRAVSDTAPTPLIRRFRPDVEGLRAIA
                     IGLVVLYHAGVPYLSGGYVGVDVFFVISGFVITAQLLRERVKSGSNSLREFYARRMRR
                     IVPAASLVIVATTVISFLALGAVRGRQIAQDGLWSSFFAANIHLAAVGTNYLSAERPP
                     SPLQNYWSLAVEEQFYLVWPVLMLTAAALFARRALRTKLTVLLVLVVGSSLTWSAVET
                     SRNATAAYFSPATRAWELGVGALIAVVASSLRHVPVLVAAIATWSGLLLIVYSAIMLS
                     SRTAYPGTAATMPVFGAALVIIGGTVGPRAGAEWLLRRWPMQWLGKISYSWYLWHLPI
                     LTIAQERNGTLTTHDKVGLVGLSLVAAIATYAIVENPLRMSRKLAIRPLGSVVLGLGV
                     IVATIGGVLAYTAANSGFGASSDVVVAASSGEPVTGSANAVEVAAVIATSTTIQAVPG
                     NVKPGLNHVAGDGGTAYHDGCLAEPTQVTVGTCAFGDLGSRDTLVLYGDSHAAMWLPA
                     FADYATRAQLKLVLLAHSGCPAPDMPFFDAVDHRRYPECDAWHRYAIGRINALAPKYV
                     VVTSSFDGALDPSQRKITPAAWTAGTTRLLNEIAVPNVKVAVLGDIPYPQQVTADCLA
                     AHAAAVYRCSTPRDRAVLGKNQSADADGARAGGAIYVDVNSWFCTATTCPPLAGDVVI
                     YQNAYHITATYSQLLAGVVGDAISESLGAPTGR"
     gene            complement(2502..3776)
                     /locus_tag="VGD55_06850"
     CDS             complement(2502..3776)
                     /locus_tag="VGD55_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870098.1"
                     /translation="MMRILMVSPHPTYSPRGTPISVLNRCRALSALGHEVDLVTYGIG
                     EDVPVPGLRYLRAPVPGIRQVKVGPSAAKLPLNAAVFARTLAVLLRRRSRYDVLHTHE
                     EAGPFGAVISRVFRLPHVYDMGNDLDVVLGNYGLGAGHPLRRIARSVESGTIRSADVV
                     VAHFPSLAARVRDVDPNTHCEVAFNVPLVAHVRDDLVTHYRNAWGLEGKAAVVYTGTL
                     ETYQGIDNLLDAVAVGARNFKLVLIGGSPVQQETLRTRVSTEGLADDVLVVGQLPSEH
                     IPSCLAAADILVSPRATGTNTPLKIFEYMHSGRPIVATRIPSHTAVLDDEVSILTGPE
                     PEQLAAGLAMAVNGGAAIAAKGLAAASRAIEQYGALGYLRAVASAYSRIAGFPAAESI
                     NFDAARARLAGQSDGPHTVVIPDPNSATLSNR"
     gene            complement(3773..4810)
                     /locus_tag="VGD55_06855"
     CDS             complement(3773..4810)
                     /locus_tag="VGD55_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0870099.1"
                     /translation="MDGPPLTQRHGSPKTSAHHRRILALRVFVSVALLGFLLSKAGIH
                     RLGRTLSDASIPWLLLGLGIGCAAKAAQVTQWHGLLRAVGLRRTWLRCARLDCAGNAL
                     DAALPTSIGGDIVRAFYAGVGIERVAAATSVVLRRVCNLPGLVLIMGCTTLADLPNAY
                     AGRITPYAAIGAAGGAAIVAVFTTPLLPALLRSRWLRGPKLGSIASKIHDAISMVRGR
                     QLVVASARGVQFWLLVSASQYCFMRGVGIHVPIVYAALVVTTVNAISLLPISVGGYGL
                     REGAFSAFLAVHGFATAAQGVAVGVCLTAQTLLLGLLGLPLLISLRRQATEAVRAPID
                     LREVSVRESVA"
     gene            complement(4792..5859)
                     /locus_tag="VGD55_06860"
     CDS             complement(4792..5859)
                     /locus_tag="VGD55_06860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0870100.1"
                     /translation="MSGLTVVEHPAMIEQIDPTVSVVVPLYNEEENLDPAVAEILDAL
                     DGLPEIAELILVDDGSTDGTLLKAWAWSQRDPRVRVIEFRRNFGQTAALQAGMAYARG
                     RRVVLMDGDQQNDPHDIAMLLAKMDEGYDVVSGWRRNRQDRALSRKLPSKVANGLISR
                     VTGVRLHDYGCTLKVYDAEVVRDLRLYGELHRFIPALAGMSGARVVEVPVNHRARTRG
                     KSKYGIGRMPRVLLDMFTVKFLLTYLVKPMQFFGLLGLVCFGFGLLTTIYLAGDKLLR
                     SHSLADRPLLILACSAAVVGVVLMCTGLIMELLIRVYHEVGERAPFAVRRTSDDIRLE
                     AARTSQTPRRVRGAHPWTARP"
BASE COUNT         1133 a         1852 c         1896 g         1113 t
ORIGIN      
        1 caccccctcg tgtgttcgtg tgagaaccgg cgcttcggcc cgcccccgag cgcaataaag
       61 caatttcaga tttcatgcat cgcgaatcgc ccgtacgtgc gatttcgaga agtactgaat
      121 caccgaagcc atcctggaag accgctgtta ccggtatgaa agccaggcat taagagtgcg
      181 tgaacacggg gcacacaacg ccggcttttc gttgcggcgt agttcgactc aatgatccag
      241 tggacgtttc cttgcctgtt accttacgta caccgcgatg tttcacgcac aggccatagt
      301 gggaatccgc cgacatcgga taacccgggg gggccccgcg cgtggtcgtg caactttccg
      361 gtgtcgatcg ggatcggcac gaagtgtctg tgccgcgcgc cgtgtcggat accgctccca
      421 cgccgctgat tcgtcgattc cgtccggacg tcgaaggcct gcgggcgatc gcaattgggc
      481 tcgtcgtctt gtaccacgcc ggtgtgccat atctcagcgg cggatacgtc ggggtcgacg
      541 tgttcttcgt tatctccggc tttgtcatca ccgcacaact gctgcgggag agagtgaaat
      601 cggggagcaa ctccctgagg gagttctatg cacggcgcat gcgccgcatc gtgcccgcgg
      661 cgtcgttggt catcgtcgcg acaactgtta tcagcttcct cgctcttggc gcagtgcgcg
      721 ggcgacagat cgctcaggac ggcttgtggt cgtcgttctt cgccgcgaac atccatctag
      781 cggccgtcgg caccaactac ctcagtgccg agagacctcc ttcgccattg caaaactact
      841 ggtcgctcgc agttgaggag cagttctact tggtgtggcc cgtgttgatg ctgaccgcgg
      901 ctgcactgtt cgcccgacga gcgctccgaa cgaagctcac cgtgctgttg gtgttggtgg
      961 tgggttcttc gctcacctgg tccgcagtcg aaacatcacg gaacgccacc gccgcctact
     1021 tctctccagc gacccgtgcg tgggagctcg gggtgggggc ccttatcgcg gtcgtcgcct
     1081 ccagtctgag gcacgttccg gtgcttgtcg cagcgatcgc gacgtggtcg ggcctgctcc
     1141 tgatcgtgta cagcgcaatc atgctgagca gtcggaccgc ctaccccggc acggccgcaa
     1201 cgatgcccgt attcggggcg gccctggtga tcatcggcgg tacggtcgga ccgcgcgctg
     1261 gtgcggagtg gctgcttcgt cgctggccaa tgcaatggct cggcaagatc tcctactcgt
     1321 ggtacctgtg gcacctaccg atattgacga tcgcgcagga gcgcaacggc acgctgacca
     1381 cacacgacaa ggttggactc gtcggcctgt cgctggtcgc agccatcgcg acatatgcga
     1441 tcgtcgagaa cccgctgcgc atgtctcgca agctcgccat ccggccactt gggagtgtcg
     1501 tacttgggct cggggtgatc gttgcaacga tcggtggcgt tctcgcctac accgcggcta
     1561 actcgggatt cggtgcaagc tctgatgtgg tcgtggctgc ctcgagtggt gagccggtaa
     1621 cgggttcagc caacgccgtc gaggttgctg cggtcattgc taccagcaca acgatccagg
     1681 cagtgcctgg caacgtgaag cctgggctga atcacgtcgc tggcgacgga gggaccgcgt
     1741 atcacgatgg gtgcttggct gaaccaacgc aggtgaccgt tgggacgtgc gcgttcggcg
     1801 atcttggctc ccgggacacg ctcgtgcttt atggagattc gcacgcagcg atgtggctgc
     1861 cggcgttcgc cgactatgcc acgagagcgc agctcaagct cgttctgctg gcgcacagcg
     1921 ggtgccccgc accggacatg ccgttcttcg atgcagtcga ccaccggcga tatccggagt
     1981 gcgacgcgtg gcaccggtac gcaatcgggc ggatcaatgc gttggcacct aagtacgtgg
     2041 tcgtgacgag ctcattcgat ggtgcactgg atccgagcca gcgcaaaatc actcccgcgg
     2101 catggacggc gggcacgact cgcctgctga atgagatagc agttccgaac gtcaaggtgg
     2161 cagtcctcgg cgacatcccg tatccgcagc aggtgacggc cgattgtctg gctgcgcacg
     2221 ctgccgcggt gtaccggtgc tcgacgccgc gagaccgagc ggttctcggc aagaaccagt
     2281 cggcggatgc cgatggcgca cgcgctggag gcgcaatata cgtcgacgtc aacagctggt
     2341 tctgcaccgc aacgacgtgt ccgccccttg ctggcgatgt cgtgatctat cagaacgcct
     2401 accacatcac cgcgacgtac tcgcaactct tggcgggagt cgtcggggac gcgatctctg
     2461 agtcgctcgg cgcgccaacc ggccgctagc gacaactgct gtcagcggtt tgacagcgtc
     2521 gcggagtttg gatctgggat cacgaccgta tgaggtccat ccgactgacc ggccaacctc
     2581 gcacgtgccg catcgaagtt aatcgactcc gcagcgggga agcctgcgat ccgtgagtac
     2641 gcgctggcaa ctgctcgaag gtatccgagc gcgccgtact gctcgatcgc tcgagatgcc
     2701 gctgcgagcc ccttagccgc tatcgccgcc ccaccgttca ccgccatcgc caagccggca
     2761 gcgagttgtt caggctcagg tccggtgagg atcgatacct cgtcatcgag caccgcggtg
     2821 tggctcggaa tgcgagtggc gacaatcgga cgcccgctgt gcatgtactc gaagatcttg
     2881 agtggagtgt tggtgcccgt cgccctcggc gacacgagta tgtcagccgc cgccagacac
     2941 gacggaatgt gttcgctcgg gagctgaccc acgacgagca cgtcgtcggc caaaccttcg
     3001 gtgctcacgc gggtgcgaag cgtctcctgt tgaacgggag agcctccgat caggacgagc
     3061 ttgaagttgc gtgcgccgac ggcaacagcg tcgagcaagt tgtcgatccc ctggtaggtc
     3121 tcgagtgtcc cggtgtagac aacggcggct ttaccttcca agccccacgc gtttcgatag
     3181 tgagtcacca ggtcgtcacg gacgtgtgcg accaagggca cgttgaacgc aacctcgcag
     3241 tgggtgttcg gatctacgtc gcggacgcgt gcggccaaac tcgggaagtg cgcaacaacc
     3301 acgtcagctg agcggatcgt gccactttca acgctccgcg cgatccgtcg aaggggatga
     3361 ccggcgccca gtccgtagtt gccaaggacc acatcgaggt cgtttcccat gtcgtagacg
     3421 tgaggaagtc gaaagacgcg actgatgacg gctccgaaag ggccggcttc ctcgtgggtg
     3481 tgcagcacgt cgtatcgaga tcgacgacgg agcagtaccg caagtgttcg cgcaaacacc
     3541 gctgcattca gagggagctt ggcagctgac gggcctacct tgacttgacg aatgccgggt
     3601 accggcgcgc gcaggtagcg aaggcccggc accgggacat cctcgcctat cccgtacgtc
     3661 acgaggtcca cctcgtgacc caaggcagag agtgcccgac agcggttgag cacgcttatt
     3721 ggtgtacccc gcggtgagta cgtggggtgc ggcgagacca tcaggatccg catcatgcaa
     3781 cgctttcgcg tacagagacc tcacgcagat caattggcgc gcgaacggct tcggtggctt
     3841 gtctgcgcag gctgatcagc agcgggagac ccaagagccc gagcagcaac gtttgagctg
     3901 tcagacatac gccgaccgcg acgccttgtg ccgctgtcgc aaacccgtgc acggcgagga
     3961 acgcgctgaa cgctccctcg cgaagcccgt accccccgac cgagatcggc aacaacgaga
     4021 tcgcgttgac ggttgtcacg acaagtgcgg cataaacgat tggaacatga attcccacgc
     4081 cacgcatgaa gcaatactga ctcgccgaca caagcagcca gaactgcact ccgcgggcgc
     4141 tggcgacgac gagctggcgg ccgcgaacca tgctgatcgc gtcgtgaatc ttgcttgcga
     4201 tgctgccgag ctttgggccc cggagccagc gcgagcgcag cagcgcgggc agcagtggtg
     4261 tcgtgaacac cgcgacgatg gctgcgcctc ctgcagcgcc gatcgccgcg tacggtgtga
     4321 tccttcccgc ataggcattg gggaggtcag cgagtgtcgt gcatcccatg atgagcacaa
     4381 ggcccggtag gttgcagacg cgacgaagga ccacactcgt ggcggcggcg actcgttcga
     4441 tccctacacc agcgtaaaac gctcttacga tgtcgccgcc gatactcgtg ggcaacgcgg
     4501 cgtccaaagc attcccggca cagtcgagcc tggcgcaccg cagccacgtt cgccgcaggc
     4561 caacggcgcg aagcagcccg tgccattgcg tgacttgggc tgccttcgcc gcacatccaa
     4621 taccgaggcc taggagaagc caaggaatag atgcatcgct aagtgtgcgt ccgagccggt
     4681 gaatgcccgc tttgctcagg aggaacccga gaagcgcgac tgacacgaac acccgaagcg
     4741 ccaggatccg gcggtgatgc gcactcgttt tgggcgagcc gtgtcgttgc gtcaagggcg
     4801 ggccgtccac gggtgcgcac ctctcacccg tcggggagtt tggctcgtcc gagcggcttc
     4861 gagtcggatg tcgtcggagg tacggcgaac ggcgaacggt gccctctcgc ctacctcgtg
     4921 atagacgcga atgagaagtt ccatgatgag gcccgtgcac atcagaacca cgcccacgac
     4981 tgcggcactg caggcgagga tgagaagtgg gcgatccgcg agtgagtgtg agcgaaggag
     5041 cttgtcgccg gcaaggtaaa tcgtcgtaag gagcccgaag ccaaaacaga cgagaccgag
     5101 cagtccgaag aactgcatag gcttgacgag atacgtcagc aggaacttga cggtgaacat
     5161 gtcgaggaga actcggggca ttcggccgat gccgtacttg ctcttgccgc gagtacgtgc
     5221 gcgatggttg acaggaactt cgacaacccg tgcacctgac atgccagcca gtgccggaat
     5281 gaagcggtga agctcgccgt acagtctcag atcgcggacg acctcggcgt cgtagacctt
     5341 gagcgtgcag ccgtagtcgt gcaggcgtac gcccgtcacc cgtgagatca gcccgttggc
     5401 cacttttgaa ggcagcttcc gcgacagcgc acgatcctga cgattgcggc gccacccgct
     5461 gacgacgtcg taaccttcat ccatcttcgc cagcagcatg gcaatatcgt gggggtcgtt
     5521 ttgctgatcg ccgtccatga gcacgacccg acgtccgcgg gcatacgcca tgcccgcctg
     5581 cagtgccgcg gtctgaccaa agttccgtcg gaactcgatc acgcggacgc gagggtcgcg
     5641 ttgactccac gcccatgcct tgagcagtgt gccgtccgtg ctgccgtcgt cgacaaggat
     5701 gagttcggcg atctcgggca acccgtcaag agcgtcgagg atctcggcga ctgccggatc
     5761 caggttctcc tcttcgttgt agagaggcac aacaacggaa acggtcggat cgatttgctc
     5821 aatcatcgcc ggatgctcga caacagtgag gccgctcata ccgcaacctc ttcgacgtca
     5881 ctggactgtt gcgtgatgtc gatagcagtc ggcgcctgcg cgagccgaga gagccgggag
     5941 tccgaagaac ggttcacact tcggtaccag ccgacgaagc gctcgatacc ctct
//
LOCUS       DASWVF010000381         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_392427, whole genome shotgun sequence.
ACCESSION   DASWVF010000381 DASWVF010000000
VERSION     DASWVF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392427"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGD55_06865"
     CDS             complement(<1..245)
                     /locus_tag="VGD55_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEY0870101.1"
                     /translation="MSAASLFHETFGRSPAGVWSAPGRVNLVGEHTDYNGGYVLPFAI
                     NLRTYAAVSPRDDRRARVLSLQRPGDDVEVALEELRPG"
     gene            complement(242..1210)
                     /gene="galE"
                     /locus_tag="VGD55_06870"
     CDS             complement(242..1210)
                     /gene="galE"
                     /locus_tag="VGD55_06870"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0870102.1"
                     /translation="MKLLVTGGAGYIGSVVSRLLLDEGHIVTVLDNLSTGHADAVADG
                     ATFVEGDISHASVVLSTGDYDGVLHFAAKSLVGESMLKPSLYWRNNVGGTRDLLDAVT
                     AYGVPRFVFSSTAAVYGEPEEIPIRETAATSPTNPYGASKLAVDLMLRDEAAAHGLGA
                     ISLRYFNVAGASRGAGERHATETHLIPIALDAAAGRRPALDVFGTDWPTPDGTCIRDY
                     VHVEDLARAHLLALMATEPGTHVVCNLGNGDGFSVREVIDVVAAVTDRPVPVREVGRR
                     PGDPARLIASHDFASTHLGWTPIADLRRIVSDAWDFHRALRARETS"
     gene            complement(1254..>1770)
                     /locus_tag="VGD55_06875"
     CDS             complement(1254..>1770)
                     /locus_tag="VGD55_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0870103.1"
                     /translation="LTSARAHRDATGRNLFADVLEAEIASGERIVVSTPCWTAFVPFS
                     AHWPMEVHLYPNRQVPDLTSLTDDERADLSRVYLEVLRRMEGVHDDSLPYIAAWHQAP
                     VTTDRDLAYLHLEVFSIRRAPGKLKYLAGSESAMGAFVNDVLPEEAAKLLRAVTPEIA
                     DLVADLAPELS"
BASE COUNT          331 a          565 c          587 g          287 t
ORIGIN      
        1 ccaggacgca gctcttcgag cgcaacttcg acgtcgtcgc cgggccgttg cagtgacagc
       61 acgcgcgctc gccggtcgtc ccttggcgac accgcagcgt aagtgcgcag gttgatcgcg
      121 aacggcagga cgtagccgcc gttgtagtcg gtgtgttcgc caaccaggtt gacgcgaccc
      181 ggcgccgacc agacgcctgc gggtgaccgg ccgaacgtct catgaaacag gctcgccgcg
      241 ctcacgaggt ctccctggct cttagcgcac ggtggaaatc ccatgcgtcg gagacgatac
      301 ggcgaagatc cgcaatgggc gtccaaccaa ggtgtgtcga ggcaaagtcg tgtgacgcga
      361 tgagacgggc tggatcgcca ggtcggcgcc cgacctcgcg caccggaacc gggcgatccg
      421 tcactgccgc tacgacgtcg atgacctcac gcacggagaa gccgtcaccg ttgccgaggt
      481 tgcacaccac gtgggtgccc ggctcggttg ccatgagtgc gagcaggtgg gcacgtgcga
      541 ggtcttcgac gtgcacatag tcgcgaatgc aggtgccgtc gggtgttggc cagtcagtgc
      601 cgaacacatc gagagccgga cgccgaccgg ccgcggcgtc cagagcgatc ggaatcaggt
      661 gcgtttctgt tgcgtgccgc tcacccgccc cgcgcgacgc gccagcgacg ttgaaatacc
      721 gcaagctgat cgcgccgaga ccgtgtgccg cggcctcgtc acgcaacatc agatcaaccg
      781 cgagcttcga tgcaccataa ggattcgtcg gcgatgttgc agccgtctcg cgaatcggta
      841 tctcctccgg ttcgccgtaa accgctgctg tcgaagagaa aacgaatcgc gggaccccgt
      901 atgcagtgac ggcgtcgagc aggtcgcggg tgccgccgac gttgtttcgc cagtagagcg
      961 agggcttgag catcgattcg ccgacgagtg acttcgcggc gaagtgcaag acgccgtcat
     1021 agtcaccggt ggagagaaca acgcttgcgt gactgatgtc accttcgacg aaggtagcgc
     1081 cgtctgcaac ggcgtctgcg tggccggtcg acagattgtc gagcaccgtg acgatgtggc
     1141 cctcgtcgag caacaggcgg ctgacgacgc tgccgatata tcccgcgccg ccggtgacga
     1201 gcagcttcac cgcgactgat ccaaggacgg acccaaggac ggacccaagg cgatcaggac
     1261 agctccgggg ccaagtctgc taccaggtcg gcgatctcgg gtgtgactgc gcgcaacagc
     1321 ttggccgcct cctctggcaa gacgtcgttg acgaacgcac ccatcgccga ctccgaaccc
     1381 gccaagtact tcaacttgcc cggcgcccgc cggatcgaga acacctcaag atgaagatag
     1441 gccaggtcac ggtccgtcgt gaccggagcc tggtgccacg cggcgatgta ggggagcgag
     1501 tcgtcgtgca cgccttccat gcggcgcagt acctcaaggt agacgcggct gaggtcagcc
     1561 cgttcgtcgt cggtcaacga ggtcaggtcg ggcacctgcc ggttggggta gaggtgcacc
     1621 tccatcggcc agtgtgcaga gaagggtacg aaggcggtcc agcacggcgt cgacaccacg
     1681 atgcgctcgc cgcttgcgat ctcggcctcg agcacatcgg cgaacaggtt gcgcccggtc
     1741 gcgtcacgat gggcgcgcgc actcgtcagc
//
LOCUS       DASWVF010000382         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_290320, whole genome shotgun sequence.
ACCESSION   DASWVF010000382 DASWVF010000000
VERSION     DASWVF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290320"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VGD55_06880"
     CDS             <1..1288
                     /locus_tag="VGD55_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870104.1"
                     /translation="AVLTADRQAGAMDLLGLAGRSRLAQVVRVRGVRRLLGFALVAEL
                     PAGMTALAVVLRITQSGGSYARAGLVGAVGAIGVGVCAPMWSRLVDRRGQTVVLIPTA
                     IAVASAGAFLAVLPPRGALAPLLIASALMGLFQPPALNCARTLWPSVVTDPTLLETTY
                     SLDSSLTELVFIVGPLLAVTINAVLGSSAAVASSGLLAGLGALGLASTKASRQARGAP
                     KMEKARGALRSVGVRFLVLSVFTMVIGFAAIDVSTIATARRSAGNGAAGALIAVWGIG
                     GLLGGMAFGMRTWPGRMSTRILIFITLITVFTVALIPITNLVILGALLFIGGLNYAPC
                     FSCINQVVQRIALPGAATESFAWITSGALIGAALGEAAAGFAITHQSPHAGYVVASAA
                     LVVACGVALLGRRAVSAGDVVRAVDVEPREVVSGRA"
     gene            complement(1272..2996)
                     /locus_tag="VGD55_06885"
     CDS             complement(1272..2996)
                     /locus_tag="VGD55_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870105.1"
                     /translation="MFDSSLTWLTSCDKRSKRAPRPSQRRNPGARPGADGPDSTLVGH
                     IDVVQIHHVLPNGRLLLDDVSFRVGEGSKVALVGANGAGKTTLLRFIAGDDKPQAGTI
                     GRSGGLGVMRQFIGSVNDDTTVQDLLLSISPPRVRSAGAEVAEAELAMMETDDEPTQL
                     RYAHALASWGDAGGYQAEVEWDVCTVQALGLTFDACRYRRVGTLSGGEQKRLVLEALL
                     RGPDEVLLLDEPDNYLDVPGKRWLEERLLESPKSVLLISHDRELLDRVANRIVTIEGG
                     TAWTHGGGFASYHQARHDRHDRLDEVRRRWDEEHQRLIELVKTLQQQAKNSVAMASKY
                     HAMQTRLAKFEEAGPPPSAPPPQKVAMRLRGGRTGLRAVTCEQLELSGLVAPFDVEIF
                     YGERVAVLGANGSGKSHFLRLLAGDRVRHTGVAKLGARVVPGWFAQTHEHAELAGRTL
                     ADVLWADRAHDRGRAMNVLRRYELDGQADQPFDTLSGGQQARFQILQLELSGATLLLL
                     DEPTDNLDVASAEALEDALEAFDGTVVAVTHDRWFARSFERFLIFGSDATVTESSEPV
                     WDAVRPVR"
     gene            complement(2917..5256)
                     /gene="lon"
                     /locus_tag="VGD55_06890"
     CDS             complement(2917..5256)
                     /gene="lon"
                     /locus_tag="VGD55_06890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0870106.1"
                     /translation="MSKTGTSGVVELPVLWLDDEVILPGMVAPIALDPGTQAAVDASR
                     NAADSRLLIVPRIDGAYASHGVLAIIEQVGRLPQGGQAVVVRGVQRAKIGAGVAGAGA
                     ALWVQATLIEDSPSTPRTSELATDYRALVISILQLRGAYQVIDTVQRVNDAAALSDLA
                     GYASYLDTEQKLWLLETADVDVRLEELSKWARAQLAELEVSGRIRDEVREGMEKTQRE
                     FLLRQQLAAIRKELGEDEPDGAEDYRARVEKAELPDKVREAALREVGRLERGSDQSPE
                     AGWIRTWLDTVLELPWNTRTQDSTDVTAARAILDADHEGLDDVKDRIVEHLAVRARRA
                     ERGLEIVGGRGSGAVLALVGPPGVGKTSLGESVARALDRTFVRVALGGVRDEAEIRGH
                     RRTYVGALPGRIVRAIREAGSMNPVVLLDEVDKIGSDYRGDPASALLEVLDPAQNHTF
                     RDHYLELELDLSDVLFLATANVLETIPDALLDRMEVVRLDGYTEDEKVAIARNHLLPR
                     QLSRAALDANEVDVDDEALRLIAGEYTREAGVRQLERAIARVLRKAATRLASDPENVP
                     VHVTASTLTEFLGRPRFTPESAERTAVPGVATGLAMTGAGGDVLFIEATAMAGETGLT
                     LTGQLGDVMKESAHIALSYLRAHGSELGIDPSVFDRAIHLHVPAGAVPKDGPSAGVTM
                     VTALASLATGRPVRDDVGMTGEVTLNGRVLPIGGVKQKLLAAHRAGLKTIFLPQRNGP
                     DLDDVPAAVLAALDVRLVADVADIVRQALEARAEAVAAA"
BASE COUNT          817 a         1872 c         1807 g          942 t
ORIGIN      
        1 cgcagtcctg acggcggaca ggcaggctgg agctatggat ctccttggcc tggccggccg
       61 atcaaggctg gcccaggtgg tgcgcgtccg cggcgtgcgg cggctgctcg gctttgccct
      121 ggtcgccgag ctgcccgccg gcatgaccgc gctcgccgtc gtccttcgaa tcacccaatc
      181 aggcggttcc tacgctcggg ccggcctcgt cggcgctgtg ggagcgatcg gcgtcggagt
      241 ctgcgccccc atgtggtcgc ggctggtcga ccggcgcggg cagaccgtgg tgttgatccc
      301 gaccgcgatc gccgtcgcca gcgccggggc cttcctggcg gtcctgccgc cgcgcggagc
      361 tctcgcgccg ctgctcatcg cgtcggccct gatgggactc ttccagccgc cggcactcaa
      421 ctgtgcccgc acgctctggc cgtcggtcgt gaccgatccg acgctgctcg agaccaccta
      481 cagccttgat tcgagcctga ccgagctggt gttcatcgtc ggtcccctgc tcgccgtgac
      541 gatcaacgcg gtgctcgggt cgtcggccgc cgtcgcctcc tccggccttc ttgcaggtct
      601 tggcgcgctc ggattggcgt cgacgaaggc ttctcggcag gcgcgcggcg cgccgaagat
      661 ggagaaggca cgcggggcgc tgcgctcggt cggcgtccgg ttcctggtgc tgagcgtctt
      721 cacgatggtc atcgggttcg cggccatcga cgtgtcgacg atcgcgaccg cgcggcggag
      781 cgcgggcaac ggcgcggccg gcgccctgat cgcggtctgg gggatcgggg gcctgctggg
      841 cggcatggcc ttcggcatgc gcacctggcc aggccggatg tccacgcgga tcctgatctt
      901 catcacgctg atcacggtgt tcaccgtcgc cttgattccg attacgaatc tggtgattct
      961 cggggcgctg ctcttcatcg gaggcctcaa ctacgcaccg tgcttttcgt gcatcaatca
     1021 ggtcgtccag cgcatcgcac tacccggagc cgcgacggag tcattcgcgt ggatcaccag
     1081 tggtgcgctg atcggagcgg cgctgggcga ggcggctgcg ggtttcgcga tcacccacca
     1141 aagcccgcat gcgggctacg tcgtcgcgtc tgcagcgttg gtggtggcgt gtggggtcgc
     1201 gctactcgga cgtcgagccg tgagcgccgg cgacgtcgtc cgggcggtcg acgtcgaacc
     1261 ccgcgaagtc gtcagcggac gggcctgacg gcgtcccaca ccggctccga cgattccgtc
     1321 acggtcgcat ctgacccgaa gatcaggaaa cgttcgaacg agcgggcgaa ccaccggtcg
     1381 tgagtcaccg cgaccaccgt gccgtcgaac gcttctaacg cgtcctcgag cgcctcggcg
     1441 ctcgcaacgt cgaggttgtc ggtgggctcg tcgagcaaca gcaatgtcgc acccgacaac
     1501 tcaagttgca ggatctggaa ccgcgcctgc tgcccgcccg acaacgtgtc gaagggctgg
     1561 tcggcttggc cgtcgagttc gtagcgccgc agcacattca tcgcgcggcc gcgatcgtgt
     1621 gcgcgatcgg cccacaagac gtcggcgagg gtgcgaccgg cgagctcggc gtgctcgtgg
     1681 gtctgtgcga accagccagg gacgacgcgt gcgccgagct tcgctacccc ggtgtgccgc
     1741 acccggtcgc cggccagcag tcgcaggaag tgcgactttc cggagccgtt cgcgccgagc
     1801 accgccacgc gctcgccgta gaaaatctcg acgtcgaacg gcgcgacgag tccgctcagt
     1861 tcgagttgct cgcaggtcac cgcacgaagc ccagtgcgtc cgccgcgtag ccgcatcgcc
     1921 accttctgcg gtggaggggc ggacggcggt gggccggcct cctcgaattt cgcgagcctt
     1981 gtctgcatcg cgtggtactt gcttgccatc gcaacggagt tcttcgcctg ctgctgaagg
     2041 gttttgacca gctcgatcag tcgttggtgt tcttcgtccc accgcctgcg aacctcgtcg
     2101 aggcggtcgt ggcgatcgtg cctggcctgg tgataggaag cgaacccacc gccgtgagtc
     2161 cacgccgtcc cgccctcgat tgtgacgatg cgattcgcga cgcggtcgag gagttcgcgg
     2221 tcgtgggaga tcagcagcac cgatttgggt gactcgagca ggcgttcttc gagccagcgc
     2281 ttgccaggga cgtcgaggta gttgtcgggc tcgtcgagca acaagacctc gtcgggcccg
     2341 cgcaacaacg cctctaggac gagtcgcttc tgctcaccgc ccgagagtgt gccgacccgg
     2401 cggtagcggc acgcatcgaa ggtcagcccg agtgcctgga ccgtgcagac gtcccactcg
     2461 acctcggcct ggtaaccgcc cgcatcgccc cacgaggcaa gtgcgtgcgc gtagcgaagt
     2521 tgggtgggtt cgtcgtcggt ctccatcatc gcgagctcgg cttcggcaac ctccgctccg
     2581 gccgaccgga ctctgggcgg cgagatcgac aagagtaggt cttgcacggt ggtgtcgtcg
     2641 ttgaccgaac cgatgaattg ccgcatgacg ccgagaccgc cgctgcgtcc gatcgtgccc
     2701 gcctgcggct tgtcgtcgcc ggcgatgaat cgcaacaggg tcgtcttgcc ggcgccgttc
     2761 gcgccgacga gggcaacctt ggatccctcg ccgacgcgaa agctcacgtc atcgagcagc
     2821 aagcggccgt tcggtagaac atggtgaatc tggacgacgt cgatgtgacc cacgagcgtc
     2881 gagtctgggc catcggcgcc agggcgcgca cccgggttac gccgctgcga cggcctcggc
     2941 gcgcgcttcg agcgcttgtc gcacgatgtc agccacgtca gcgacgagtc gaacatcgag
     3001 cgctgcaagc accgccgccg gaacgtcgtc gaggtcagga ccattgcgct gcggcaagaa
     3061 gatggtcttc aacccggcac ggtgagccgc gagcagcttc tgcttgacgc cgccgatcgg
     3121 tagcacccgg ccgttgagtg tgacctcacc cgtcatgccg acgtcgtcac gcaccggacg
     3181 tcctgttgca agcgacgcga gcgccgtgac catcgtgacg ccggccgacg ggccgtcctt
     3241 cggcaccgcg cccgcgggca cgtgcaggtg gatggctcgg tcgaacaccg atgggtcgat
     3301 cccgagctcc gaaccgtgcg ccctcaggta cgacaacgcg atgtgtgcgg actccttcat
     3361 gacgtcaccg agctggccgg tcaacgtgag cccggtttca ccggccatcg ccgtggcctc
     3421 gatgaacagc acgtcgccac cagcgccggt cattgcaaga ccagtcgcga caccagggac
     3481 ggcggtccgt tctgcgctct cgggcgtgaa cctcggtcgc ccgagaaact ccgtgagcgt
     3541 gctggcggtg acatgcaccg gcacgttctc cggatcggac gccagtcggg tcgccgcctt
     3601 gcgcagtacc cgggcgatcg cccgctcgag ctgacgcacg ccagcttcgc gggtgtattc
     3661 gcccgcgatc aggcgcaacg cctcgtcgtc gacgtcgacc tcgtttgcat cgagtgctgc
     3721 ccgcgacaac tgccttggaa gcaggtgatt gcgcgcgata gcgaccttct cgtcctcggt
     3781 gtagccgtcg agccgcacga cctccatgcg gtcgagcagc gcatcaggga tcgtctccaa
     3841 cacattcgcg gtcgccagga acagcacgtc cgacaagtca agctcgagct cgaggtagtg
     3901 atcgcggaac gtgtggttct gcgccgggtc aagcacctca agcaacgcac tcgcggggtc
     3961 gcctcggtag tccgacccga ttttgtcgac ctcgtcgagg agcacgaccg ggttcatcga
     4021 acctgcctcg cgaatcgcgc gcacgatgcg gcccggcaat gccccgacgt acgtgcgacg
     4081 gtggccacgg atctcggcct catctcgcac gccgccaagg gccacgcgaa cgaacgtgcg
     4141 gtcgagagca cgcgcgacag actccccgag tgacgtctta ccgacgccgg gagggccgac
     4201 caatgcgagc acagcgccgc taccacggcc tccgacgatc tcgaggccac gttcggcacg
     4261 gcgcgcccgc acggcgaggt gctcgacgat gcggtccttc acgtcgtcca gaccttcgtg
     4321 gtcggcgtcg aggatcgcac gggcagcggt gacgtcggtg ctgtcttgcg tgcgggtgtt
     4381 ccacggcagt tcaagcaccg tgtcgagcca ggtacggatc cagccggcct cggggctctg
     4441 gtcgctaccc cgctcgagcc tgccgacctc gcgcagggcc gcctccctga ccttgtcggg
     4501 cagctcggcc ttctcgacgc gcgcccggta gtcctcggcg ccgtctggct cgtcctcgcc
     4561 gagttccttc cggatcgccg ccaactgctg gcgcagcagg aattcgcgct gcgtcttctc
     4621 catgccttca cgaacctcgt cacggatccg gccgctgacc tcaagctcgg caagctgcgc
     4681 ccgcgcccac ttcgaaagct cttccagccg gacgtcgaca tcggccgtct cgagcagcca
     4741 cagcttctgc tcggtgtcga ggtacgacgc gtacccggcg agatccgaca gcgctgccgc
     4801 gtcgttgacc cgctgaaccg tgtcgatcac ctgataggcg ccacgcagct gaaggatcga
     4861 gatcaccagg gcccggtagt cggttgcgag ctcgcttgtc cggggggttg agggcgaatc
     4921 ttcaatcaac gtcgcctgca cccacagggc cgcgcccgct ccggcgacgc cggcgccgat
     4981 tttggcgcgc tgcactccac ggacgacgac agcctggcca ccctgcggca gccgaccgac
     5041 ctgctcgatg atcgcgagca ccccatggct cgcgtacgcc ccgtcaatcc tggggacgat
     5101 cagcaaccgc gagtcggcgg cgttgcgcga cgcgtcgacg gcggcctggg tgcccggatc
     5161 gagggcgatg ggcgccacca tgcccggcag gatcacttcg tcgtccagcc acaacaccgg
     5221 tagttcaacg acgccggacg tgccagtctt tgacattgct gcctccttga gtctcctacg
     5281 ctcgactcac ggagggccac gcaacttccc gcggcgtgtt cgctgacagc gtggcgcctt
     5341 aatcgtgctg tagcagccat tcgtgcactg cgggcacacg gttgacggtc aaggtgatgt
     5401 ggccgtcgac gtccgtgagg tgggcatcga cgccgggg
//
LOCUS       DASWVF010000383         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_290327, whole genome shotgun sequence.
ACCESSION   DASWVF010000383 DASWVF010000000
VERSION     DASWVF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290327"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="hemE"
                     /locus_tag="VGD55_06895"
     CDS             complement(<1..815)
                     /gene="hemE"
                     /locus_tag="VGD55_06895"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0870107.1"
                     /translation="MTDSSVFLRACRGEAVEHPPVWFMRQAGRALPEYRAARDGIPML
                     QACMTPDLVAEVTLQPVRRYGVDAAIIFSDIVVPLKAIGVDLDIVAGVGPVLATPITT
                     SRDVEELRPLVPEDVEFLDTAVRLVVAELGPTPLIGFAGAPFTLASYLVEGGPSREHL
                     KTKSLMYTQPALWHDLLDRLATIAIEFLRVQVGAGARAVQLFDSWAGVLSPADYRRYV
                     LPHSARVLETLEDLAVPRIHFGVGTGELLGLMGDAGADVVGVDWRVPLDVAAT"
     gene            complement(850..1743)
                     /locus_tag="VGD55_06900"
     CDS             complement(850..1743)
                     /locus_tag="VGD55_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0870108.1"
                     /translation="MTAVLALLASLVWGTSDFMGGVVSRRVRPLLVVAIAHTIALATL
                     LVVAAVAGTFSAKLGYLPWSVAAGAVGLISLVAFYGALSTGTMGIVAPIAGTGAVVPV
                     VVGLASGESPSGWQIAGIVAAVAGVVLASGPELTGRGRADGGGSRPLVLAVIAAVGFG
                     TVFVLIDRAARHNLVMTLLTMRVTSVSIALLLGLGFLVRRRRRVDAAIDDEPVAREWG
                     VHWKQVPAIAIVGWTDAGANALYGAAVRHGLVSVTAVLASLYPAVTVLLARFIEGERM
                     RPIQNIGVGLAMCGVVLLAAA"
     gene            1833..2417
                     /locus_tag="VGD55_06905"
     CDS             1833..2417
                     /locus_tag="VGD55_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HEY0870109.1"
                     /translation="MAARDPHSELPAGFAKAVASLRPVRLRAEIRLAEAPAPQRLAPF
                     AVALTADVSDTDGNDTATGRFVVLHDPAGQEAWQGSTRIVAYVRAELEQEMASDPFLA
                     DVAWTWLTEGLARRLPERGYRAVGGTVTRVTSECYGTMAGRPLVAHVELRVSWTPDND
                     DLGLHLEAWGDVLAAAAGLAPLPPGVAALPNRRR"
     gene            2625..3242
                     /locus_tag="VGD55_06910"
     CDS             2625..3242
                     /locus_tag="VGD55_06910"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEY0870110.1"
                     /translation="MSLPNRQPRPIPAWYADPWAVGMLRWWDGATWTGFTQSPPHQRL
                     AFLKELPWQVWVWWLGILAASVIVCFGAAESTTVAHPGRWLLAGFAIAAALTAGCAAS
                     TLAFSRWRDLLVFGVIVAAITSLALFIPGTVSTSRSCQNAGQPTSSGTHDCDTSDVIG
                     GPVIFVVLCVPSLALAGIGKGVGRLVRRPGNTPTVTALGRWRNSR"
BASE COUNT          596 a         1182 c         1134 g          503 t
ORIGIN      
        1 ctggtcgctg cgacatcgag cggcacccgc caatcgacac cgacaacgtc ggcgcctgcg
       61 tcgcccatca ggccgagcaa ctccccagta ccgacaccga aatgaatccg aggcacggcc
      121 agatcttcga gcgtctcaag cacgcgcgca ctgtgcggaa gcacgtaacg ccgatagtcg
      181 gccggagata gaacgccggc ccacgaatca aagagttgga ccgcgcgggc tcccgcaccc
      241 acctgaacgc gcaggaactc gatcgcaata gttgcgagcc ggtcgagcaa gtcgtgccat
      301 agtgcgggtt gcgtatacat caacgacttg gtcttcagat gctcgcgcga cgggccgccc
      361 tcgacgaggt aactggccag cgtgaagggc gctcccgcga acccgatgag cggtgtcggt
      421 ccgagttcgg caaccaccaa acgcactgct gtatcgagga attcgacatc ttcaggtacc
      481 agtggccgca actcctcgac gtcccggctg gtagttatcg gcgtcgcgag aacaggaccg
      541 acacccgcga cgatgtcgag gtcgacgcct atcgccttca acggcaccac gatgtcgctg
      601 aagatgatcg ccgcatcaac tccgtaccgg cgcaccggct gcaacgtgac ctcggcaacc
      661 agatcggggg tcatacaggc ctgcagcatc ggaatgccgt cgcgggccgc gcggtactcg
      721 ggaagcgcac ggccggcttg ccgcatgaac cacaccggcg ggtgctcgac ggcttcgccg
      781 cggcaggccc ggaggaacac gctgctgtca gtcattgccc catgctgtca ggcggccgcg
      841 aggaggctgt caggcggccg cgagcagcac gacgccacac atcgccaggc ccacgccgat
      901 gttctgaatc ggccgcatcc gctcgccctc gatgaatcgc gcgagcagca cggtgacggc
      961 tgggtaaagc gatgccagca ctgcagtgac gctcaccagg ccgtgccgga cggcggctcc
     1021 gtagagggcg ttcgccccgg catcggtcca gccgacgatc gcgatcgcgg gcacctgctt
     1081 ccagtgcacg ccccactcgc gcgcaacggg ttcgtcatcg atggcggcgt cgacgcgccg
     1141 ccgccggcgc accagaaagc caaggccgag caacaacgcg atcgacacgc tcgtgacacg
     1201 catcgtgagc aaggtcatca ccaagttgtg cctggccgcg cgatcgatca ggacgaacac
     1261 cgtgccgaat ccgacggccg caataaccgc cagcacgagc ggccgcgatc cgccgccatc
     1321 ggcgcgacca cgcccggtca actcaggccc acttgcgagg acgacgccgg caaccgccgc
     1381 cacgatcccc gcgatctgcc acccggaggg agactccccc gacgccagcc cgaccaccac
     1441 cggtaccacc gcaccggtgc ccgcgatcgg ggcgacgatc cccatcgtgc cggtcgacag
     1501 ggcgccgtag aacgcgacaa gggagatcaa gccgaccgcg ccggcggcca ccgaccacgg
     1561 caggtagccc agcttcgcac tgaaggttcc ggccaccgcc gccacgacga ggagcgtggc
     1621 gagggcgatg gtgtgggcga tcgcgacgac cagcaacggc cgcactcgcc ggctcacgac
     1681 cccacccatg aagtcggacg tgccccatac aaggctcgcg agaagcgcga gcacggctgt
     1741 catggcggcc gagcctacgg gagcaggccc acctggcgcc tggtcggcgc gccgcctccc
     1801 gggccggcgc aatggcacct agattgcgag atgtggcagc cagagatccg cacagcgaac
     1861 tgcccgcagg ctttgcgaag gcggtcgcga gcctgcgtcc ggtccggctg cgcgccgaga
     1921 tccggctcgc cgaggcgccc gccccgcagc gactcgcgcc gttcgcggtg gctctgacgg
     1981 ccgacgtcag cgatacggac ggcaacgaca cggctacggg ccgtttcgtc gtcctgcacg
     2041 accccgcggg ccaggaggcc tggcagggct ccactcggat cgtcgcgtac gtgcgcgccg
     2101 aactcgagca ggagatggcc agcgacccgt tcctcgccga cgtcgcctgg acctggctca
     2161 ccgaaggtct cgcaaggcgg ctccccgagc gcggctaccg tgcagtcggt ggcaccgtca
     2221 ccagggtgac gtcggagtgt tacggcacga tggccggccg tccgcttgtc gcccacgtcg
     2281 aactgcgggt gtcgtggacg cccgacaacg acgatctagg cctgcacctc gaagcatggg
     2341 gcgacgtgct cgccgccgca gccggtcttg cgccactgcc tccaggagtt gcggcactgc
     2401 caaaccgccg ccgctaacca gggctagcta ggagtcctgc cgttgcttcg acaagagaaa
     2461 cgccaggagc ccggcggcgc ggcctatccc agccgtcgag gcgagggcct ggcgggccgt
     2521 catgctgcgc gttcaggtga acgcgcacgg cagcgaagtc ggatccgcgc cgtgggctta
     2581 cgaaacctgg tcatacctcg gcgtgcgtgc gagatgatcg agccgtgtcc ttgcccaatc
     2641 ggcagccccg cccgattcct gcgtggtacg cggatccgtg ggcagtcggg atgctcaggt
     2701 ggtgggacgg cgcgacgtgg acggggttca cgcaatcgcc gcctcaccag cggctggcct
     2761 tcctgaaaga gttgccgtgg caggtatggg tttggtggct tgggattctg gccgcctccg
     2821 tgatcgtctg cttcggcgcg gcggagtcga cgacggtggc tcaccccgga cggtggctgc
     2881 tagcggggtt tgcgattgcc gcagcactga cggctggttg cgcggcatcg acgttggcgt
     2941 ttagtcgctg gcgagacctt ctcgtgttcg gggtcatcgt ggctgcgatc acgtcactgg
     3001 cgctgttcat ccccggcacc gtgtcgacga gtcgtagctg tcagaacgcg ggacagccga
     3061 ccagttctgg tacccacgac tgcgacacgt ccgacgtgat tggcggccca gtgatattcg
     3121 tcgtcctgtg tgttccatcg ctcgcgcttg ccgggatcgg aaagggcgtt ggccggttgg
     3181 tccgcagacc tggcaatacg ccaacggtta ctgcgctggg ccgctggcga aattctcggt
     3241 aagaaggcga gccctggctg atctgcgcga atctagccgg cgacggcggg ccggctcgac
     3301 gagttagccg acctgacctc gcccgctgtc ggcgcgccgg ccagtgtgcc gcggcccatg
     3361 cgcagagtca gcgtggttcc ctcaccaagc cacgagtcaa cgtcgagtga accgc
//
LOCUS       DASWVF010000384         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_290406, whole genome shotgun sequence.
ACCESSION   DASWVF010000384 DASWVF010000000
VERSION     DASWVF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290406"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGD55_06915"
     CDS             <1..507
                     /locus_tag="VGD55_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870111.1"
                     /translation="ASTIVAFLAVRLTVTYLVRPAIFSPRHLTLALDPNNMGFGSTNG
                     GPQTLMPNPPNLPNAWVYSTHVVDSAGHGLASATVATACPSLPFPGSGGGRPPGSGTR
                     VRVAGDGIQGALHDCVTKLGATYHEVVTYQPANRYWTLQWGETAVYFAAALALAALSF
                     WWIRRRAR"
     gene            536..865
                     /locus_tag="VGD55_06920"
     CDS             536..865
                     /locus_tag="VGD55_06920"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY0870112.1"
                     /translation="MAYDEDLANRIREVVGREKGLTEKRMFGGLAFLINGNMAVSASG
                     KGGLMLRVDPAETDTLVKEPAARPFEMRGREMDGWLRVDTDELDDDALTRWVRIGVTY
                     AKSLPPK"
     gene            complement(875..1645)
                     /locus_tag="VGD55_06925"
     CDS             complement(875..1645)
                     /locus_tag="VGD55_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870113.1"
                     /translation="MRSSDGSAGDVNYGRVGRAYSSYRQPDARIARLITKALGDARTV
                     VNVGAGAGSYEPTDRVVTAVEPSASMRAQRTEHMPAAVDAVAEALPFEDDSFDAAMST
                     FSVHQWNDLNAGLRELRRVTRGPVVILTCDPDLVRNFWLYDYAPEVLDTEARRYPSID
                     ALADGLEGTLSTTIVPIPLDCSDGFNEAYYGRPEQLLDPDARLSCSAWSFVDQATQER
                     FTDQLRTDLHDGTWDIRHGHLRNLPTLAGSLVLVTSRP"
     gene            1711..2097
                     /locus_tag="VGD55_06930"
     CDS             1711..2097
                     /locus_tag="VGD55_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870114.1"
                     /translation="MVTLDEVVAMASALPDVTEGARWGNRTWLVNGKGFAWERPLSKA
                     DIKRLGDRPPPEGPIIAVRVADLGEREAVLAANLPGFFTIEHFSGYPAFLIQLKKATK
                     KATREAILDAWLACAPPKLAESYLKR"
     gene            complement(2087..3445)
                     /locus_tag="VGD55_06935"
     CDS             complement(2087..3445)
                     /locus_tag="VGD55_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870115.1"
                     /translation="MRPFIILAVVLVFIVVRLTLRYRSRQPKARSVAATAPTKSLQAP
                     RRSWLVVLFGVVGLVAQREVRERIRSRVFQIGTAIILLVVVAAIVIPVLTKSKAQPDQ
                     VGVVGALPAPVRAALIASDASIGDTMTVILEEDMETADADLRAGRVDIVVVDGKQLVV
                     KDAISASDVSTAAQLARVFSATLGTIAAFQAAGLSSTQADTLSQAKPLPVTSLQAPTH
                     RRKPSPGSIISLILVFVMLTQYSTWILTGVAEEKSSRVVEVLLGALRPAQLLAGKVLG
                     IGLVALTQATLIVGVALGLSGAVGSNLLHGSGPVTVLAALLWLFLGYGFYCWMYAAAG
                     SMVERQEQIQSLAFPLSLPLIIGYVVSLITASSARPTTFLEILAYLPPTAPVAIPTLV
                     SLGGATWWEFVISVVVSLASTSAVARLATSIYRRAILRTGRRVRFREVVSARPSRPTI
                     SA"
     gene            complement(3442..4350)
                     /locus_tag="VGD55_06940"
     CDS             complement(3442..4350)
                     /locus_tag="VGD55_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870116.1"
                     /translation="MAAEVGGLLELVGLTRRYGAITALDGLSFSVQAGQVVGFLGPNG
                     AGKTTTMRAVFGLTDLESGEVRWNGAKVDQPARRRFGYMPEERGLYPGMLVGEQVAYM
                     GRLHGLTAGDAVKATNVWLERLGVAERWDSKVDVLSHGNQQRVQLAAALVHEPELIVL
                     DEPLSGLDPAGIDDIGKVLVERASAGCCVLFSSHQLDLVEDLCESVTIIDHGKLVASG
                     RVDDLATSGPRRLVVRVDGDRDATWARGLAGVAVSEINGGAVRLVLDESVDSNVVLSA
                     AMAAGRVSEFSFARRRLSEVFREALA"
     gene            4373..5005
                     /locus_tag="VGD55_06945"
     CDS             4373..5005
                     /locus_tag="VGD55_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0870117.1"
                     /translation="MVTDDSALMPFIRTIAASDASLASSLLAASPSLASAQLAVGASR
                     QEARDFYLGEIGHYAYAGDTALHIAAAGYHAGFARALVSAGANVSAKNRRGAQPLHYA
                     ADGGPGSASWNPTAQAATIAYLIEAGADPNATDNSGVAPLHRAVRTRCAAAVSALLEG
                     GADPRRANKNGSTPRSLASHNTGRGGSGAAPAKEQQREIVRLLELRGATL"
     gene            complement(5017..5217)
                     /locus_tag="VGD55_06950"
     CDS             complement(5017..5217)
                     /locus_tag="VGD55_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870118.1"
                     /translation="MDVAELADLLRETSQHHDPYEKSSPPHDWWNWYAPYMAARQQGR
                     TPEEASAAAGLYMDKIREAGSG"
     gene            5271..5714
                     /locus_tag="VGD55_06955"
     CDS             5271..5714
                     /locus_tag="VGD55_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0870119.1"
                     /translation="MDMKLEVVVLPVADVDRARSFYTGIGWRLDADFSTGDDFRVVQL
                     TPPGSQCSIIFGAGLTTAVPGSTQGLQLTVTDIEAARAELTNAGVEVSEVFHDGGGVF
                     HHAGTQGRVPGADPNHRSYASFASFSDPDGNSWFLQEITERLPGR"
BASE COUNT         1061 a         2000 c         1885 g          953 t
ORIGIN      
        1 gcatccacga tcgtggcgtt cttggctgtg cgactcaccg tcacctactt ggtgcgtccc
       61 gctatcttct cgccgcgtca tctgacgctt gcgctcgatc ccaacaacat gggcttcggc
      121 agcacaaacg gtgggccaca aacgttgatg ccgaacccgc cgaaccttcc caatgcatgg
      181 gtctactcga cgcacgtcgt cgacagcgcg ggccacgggc tcgcctccgc gaccgtggcg
      241 accgcctgcc cgagtctgcc cttccccggt agcggtggcg gccggccgcc agggtcaggt
      301 acacgcgtac gggtcgcggg cgacggcatc caaggcgcgc tccacgattg cgtcacgaaa
      361 ctcggcgcca cgtaccacga ggtcgtgacc taccagccag ccaaccgtta ttggacgctc
      421 caatggggcg agactgcggt ctatttcgcc gccgccctcg cgctggccgc cctctcgttc
      481 tggtggattc gccgccgcgc gaggtgatgt cagacggcgg cggcatactc ctgctatggc
      541 gtatgacgaa gatctggcga accgaatacg tgaggtcgtc ggcagggaga agggcttgac
      601 cgagaagcgc atgttcggcg ggctggcgtt cctgatcaac ggcaatatgg ccgtcagtgc
      661 gagcggcaag ggcggcctca tgctgcgcgt cgacccagcg gaaaccgaca ccttggtgaa
      721 agagcccgcc gcccggccgt tcgagatgcg agggcgcgag atggacggct ggcttcgggt
      781 cgacaccgac gaattggacg acgatgcgct cacgcggtgg gtccgcatcg gcgtcaccta
      841 cgccaagtca ctcccgccca agtaatcgct gcagttacgg ccggctcgtc acgagaacaa
      901 gactgccggc aagcgttggc aggttccgca ggtggccgtg gcggatatcc cacgtgccgt
      961 cgtgcaggtc agtgcgcagc tgatcggtga agcgttcttg cgtcgcttga tcgacgaagc
     1021 tccaggctga gcacgagagc ctcgcgtctg ggtcgaggag ttgttccggt cgaccgtaat
     1081 aagcctcgtt gaatccgtcc gagcaatcca gcgggatcgg cacgatcgtc gtcgacagcg
     1141 tgccttctag accgtccgcc agcgcgtcga tcgaggggta gcgcctcgcc tcggtgtcga
     1201 gtacttccgg tgcgtagtcg tacagccaga aattccgaac caggtcgggg tcacaggtga
     1261 ggatcacgac cggcccgcga gtgacgcgac ggagctcgcg cagcccggcg ttcagatcgt
     1321 tccattgatg cacgctgaag gtcgacatcg cggcgtcgaa gctgtcgtct tcgaaaggca
     1381 gcgcctcggc gaccgcgtca accgcggccg gcatgtgttc ggtgcgctgg gcccgcatcg
     1441 acgccgacgg ctcaaccgcc gtgacgacgc gatcggtcgg ttcgtacgac cccgccccgg
     1501 caccgacgtt gacgaccgtg cgcgcatctc caagggcttt cgtaatcagc cgggcgattc
     1561 gagcgtctgg ttgccggtac gagctgtacg cgcggccgac acgaccgtag ttgacatcgc
     1621 ctgcgctgcc gtccgaactc cgcacgcgca cgcccttctc aaccgatttc ccttgcgcac
     1681 agggtacttt gcgcccggta aggttcgctc atggtgacac tcgacgaggt ggtagcgatg
     1741 gcgtcggcgc tgcccgacgt caccgaaggc gcgcgctggg gcaaccgcac ctggttggtc
     1801 aacggcaaag gcttcgcgtg ggagcgacca ctaagcaagg ccgatatcaa gagactcggc
     1861 gacaggccac ctcctgaggg gccgatcatt gccgttcgcg tggccgacct gggcgagagg
     1921 gaagccgtac tggcggcgaa cctccctggc ttcttcacca tcgagcattt cagcggatac
     1981 ccggcattcc tgatccagct caagaaggcc accaagaagg ccacccgaga agcgatcctc
     2041 gacgcctggt tggcgtgcgc cccgccgaaa ctcgcggaga gctacctcaa gcgctgatcg
     2101 tgggccgcga cggcctcgca gacaccactt cacggaaccg cacccgacga ccggtccgca
     2161 gaatcgcgcg gcgatagatg gacgtcgcga ggcgagcaac cgcgctcgtg ctcgccaggc
     2221 tgacaacaac tgagatcacg aactcccacc aggtggcgcc gccaaggctg accagagtcg
     2281 gaatagcaac aggggccgtc ggtggcaggt acgccaggat ttcgaggaag gtggtaggtc
     2341 tggccgaact cgccgttatg agcgagacga cgtaaccgat gatcagcggc aggctcaacg
     2401 ggaacgcgag gctttggatc tgctcctggc gttcgaccat cgaacccgcc gcggcgtaca
     2461 tccagcagta gaagccatag ccgagaaaaa gccagagcaa ggctgcgagc acggtcactg
     2521 gtccgctgcc gtgcaacaga ttcgacccca ccgcgcccga gagcccaagc gcgaccccca
     2581 cgatcagcgt ggcctgcgtc agcgcgacga gccctatacc gagcaccttt ccggcaagaa
     2641 gctgagctgg ccgaagtgcc ccaaggagca cctcgacgac gcggctggac ttctcctcgg
     2701 cgacgccggt caggatccat gtgctgtatt gcgtgagcat cacgaacacc aagatcaggc
     2761 tgatgatgga tccaggcgac ggcttgcgcc gatgagtcgg cgcttggagg ctcgtgaccg
     2821 gcagcggttt tgcttggctg agcgtgtcgg cctgcgtgct cgacaacccg gccgcctgaa
     2881 acgcagcgat cgtgccgagc gtcgcggaaa agacgcgcgc gagctgcgcc gccgtcgaga
     2941 catcgctcgc ggagatcgcg tccttcacga cgagctgctt gccgtcgacg accacgatgt
     3001 cgactcggcc ggcgcgaaga tccgcgtcgg ctgtctccat gtcctcttcc aagatgacgg
     3061 tcatcgtgtc gccgatactc gcgtcactcg caatcagagc ggcccgcact ggtgccggca
     3121 gggcaccgac gacgccgacc tggtctggct gtgccttgct cttggtcagc accggaatga
     3181 cgatcgccgc gaccacgaca agcaagatga tcgccgtccc gatctggaag acccgcgatc
     3241 tgatgcgttc acgcacctcg cgttgcgcaa cgagacccac gacgccgaac agcacaacca
     3301 accatgaacg ccgcggcgcc tggagcgact tcgtgggggc agtggccgca actgagcgcg
     3361 ctttgggttg ccggctccga tagcgaagcg tcaaccggac cacgatgaag accaggacga
     3421 ccgcgagaat gatgaacggc ctcacgccag cgcctccctg aacacttccg acaggcgccg
     3481 gcgcgcgaaa ctgaattcac tgacccggcc cgccgccatc gccgcactga gcaccacgtt
     3541 gctgtcgacc gactcgtcga gcacaaggcg caccgcacca ccgttgattt cagatacggc
     3601 gacaccagcg agaccacgcg cccacgttgc gtcgcggtca ccgtcgacgc gcacgaccaa
     3661 ccggcgcgga ccgctcgtgg cgaggtcgtc gacgcgaccg ctcgccacca acttgccgtg
     3721 gtcgatgatc gtcacggact cgcatagatc ctcgacgaga tcgagttgat ggctggagaa
     3781 cagcacgcaa cacccagcgc tcgcgcgctc gacgagaacc ttgccgatgt cgtcgatccc
     3841 ggccgggtca agccccgaca acggttcgtc cagaacgatc aactccggct cgtgcacgag
     3901 cgctgctgcg agttgcacgc gctgctgatt gccgtgggag agaacgtcga ccttgctgtc
     3961 ccatcgctcg gcgacgccga gtcgctcgag ccacacgttc gtcgccttca ctgcgtcgcc
     4021 cgcagtcaac ccgtgcagcc gacccatgta cgcgacctgc tccccgacga gcatgcccgg
     4081 ataaaggcca cgctcttcgg gcatgtagcc gaacctgcgc cgggcaggct ggtcaacctt
     4141 cgcgccgttc cacctgacct caccggactc gaggtcggtc agcccgaaca ccgctcgcat
     4201 cgtggtcgtc ttgccggcgc cgttcgggcc gaggaatccg acgacctgac ccgcctgtac
     4261 cgagaacgac agaccatcga gggcggtgat cgcgccgtag cgacgagtca gccccaccag
     4321 ctcgagcagt cccccgacct cggcagccac ggaaccacaa taaggtgtgg cgatggtgac
     4381 cgacgactcg gcgctgatgc ccttcatacg gaccatcgcc gcgagcgatg cgtccctcgc
     4441 gtcctcgttg cttgccgctt cgccgtcgct cgcgagtgca caactcgcgg tcggggccag
     4501 ccggcaggag gcgcgcgact tctacctcgg ggagatcggc cactacgcgt atgcgggcga
     4561 cactgcttta catattgcag ctgccggcta tcacgccggg ttcgcgcgtg cgctcgtctc
     4621 ggccggcgcc aatgtcagcg cgaagaaccg acgcggcgcg cagcccctcc actacgccgc
     4681 cgacggcggg cccggctcgg cgagctggaa tccgaccgcg caggccgcga caatcgcgta
     4741 cctgatcgag gccggtgccg accccaacgc gactgacaac agcggcgtcg cgccgttgca
     4801 tcgtgccgtg cgcacccgct gcgccgccgc ggtgagcgcg ctgctcgaag gcggtgccga
     4861 tccgcgccgc gcgaataaga acggttcgac gccgaggtcg ctcgcaagtc acaacaccgg
     4921 ccgcggcgga agcggcgcgg cgccggcgaa ggagcagcaa cgggagatcg tgcggctgct
     4981 agaactgcgc ggcgcgacgc tgtagttcgc cagacgtcag cccgaccccg cctcgcgaat
     5041 cttgtccatg tagagccctg ccgctgctga ggcctcctcc ggcgtccgcc cctgctggcg
     5101 cgcggccatg tagggcgcgt accaattcca ccagtcgtgc ggcggcgacg acttctcgta
     5161 cgggtcgtgg tgttgcgatg tctcgcgcag cagatccgct agttccgcga cgtccatctg
     5221 tgccctccct cgttgtgact cgtactatcg ctgccacagg gaggtagtca atggacatga
     5281 aacttgaggt ggtcgtgctg cccgtcgccg acgttgaccg cgcgaggagc ttctacacgg
     5341 gaatcgggtg gcggctagat gccgacttct ccactggcga cgattttcgc gtcgtccagc
     5401 tcacaccgcc gggctcgcag tgctcgatca tcttcggcgc cggcctcaca acggccgtgc
     5461 cggggtcaac acagggcctc cagctgacag tgaccgatat cgaagcagcg cgtgcggagc
     5521 tcaccaacgc gggcgttgag gtcagcgagg tcttccacga cggtggtggg gtgtttcatc
     5581 acgccggtac gcagggccgg gtgcccggcg cggatccgaa ccaccgcagc tacgcgtcgt
     5641 tcgcctcgtt cagcgatccc gacggcaaca gctggttctt acaggaaatc acggagcgct
     5701 tgcccggccg gtaagcagtt gcccaacgga gtaccgaggt actccgggtc gagtctgcgc
     5761 gacgtcggcg tcgtggtgag cgcggtgaaa atcaccgatc cgcgagccac cgccacccac
     5821 ggcctatcgc cggtgccggg tcgagcccgt tcggcgcggg ctacaggcgg ctagatcagc
     5881 tgcggtttag ctgcagtcg
//
LOCUS       DASWVF010000385         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_469054, whole genome shotgun sequence.
ACCESSION   DASWVF010000385 DASWVF010000000
VERSION     DASWVF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469054"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..911)
                     /locus_tag="VGD55_06960"
     CDS             complement(102..911)
                     /locus_tag="VGD55_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0870120.1"
                     /translation="MSSVPTPDAGQSGILMWRPTEHIVELILDRPGALNAISTDLASH
                     LGGMLRALAADAAVRAVVLSSSQERAFCVGADLKERAGFSNEQLLAQRPFIKGMFAAL
                     LDLPMPVIAAVGGFALGGGFELALSCDLIVADETAVFGLTEVTVGLVPGGGGTQLLAR
                     RIGAGRAADLVLTGRKVDAPEAFRLGIVDRPATDVHVRQLALDIAATIEGNSPLAVRY
                     AKHALRSGLNTSLHDGLEIEDAAWRAAAMSADRREGISAFTEKRAPRWSGL"
     gene            complement(934..1518)
                     /locus_tag="VGD55_06965"
     CDS             complement(934..1518)
                     /locus_tag="VGD55_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0870121.1"
                     /translation="MRGMAMNLSDGEEVVLDLHPHWGRLFLPVLVLLLACLLAGFGVA
                     LIPKGGGQQIERWILIAIAAVVVIWRTILPYLRWLTTKYVLTTDRLIIRNGILARHGR
                     DIPLNRINDVSFSETVLERMLRSGTLVVESAGDNGQISLTDVPKVEHVQREVYRMVDQ
                     NLGKTGPMSVADGGRAGRGVAADPNAADEAPGQR"
     gene            1579..2130
                     /locus_tag="VGD55_06970"
     CDS             1579..2130
                     /locus_tag="VGD55_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEY0870122.1"
                     /translation="MTASVTVRAATTADAAAIADIYRPYVLETAISFEETPPTAADFE
                     ARMLATPRLPWLVAVREGAVLGYCYASRHAARASYRWAADVSVYVAGGERGRGVGRLL
                     YSELLPLVQGLGYVSVFAGITLPNDASVGFHQAFGLTPVGVYRNVGFKHSKWLDVGWW
                     QLTFTDPPDQPAEPRAWVPPALL"
BASE COUNT          390 a          760 c          676 g          363 t
ORIGIN      
        1 gtcgaccagc tcgaccttcg cggcggtcga cacaatcgcc tcgttctgca gtccgtcgcg
       61 cgggccgacc tcacgaaggc tgatacgcga aggcaggtcg ctcatagtcc cgaccacctc
      121 ggcgcacgct tttcggtaaa ggcgctgatc ccctcacgac ggtccgcgga catcgccgcg
      181 gcccgccacg cggcgtcctc gatttcaagg ccgtcgtgca atgaagtgtt gagcccacta
      241 cgcaaggcat gcttcgcata ccggacggcg aggggcgagt ttccttcgat cgtggccgcg
      301 atgtccaacg ccagttgcct gacgtgcacg tctgtcgcgg gccggtcgac gatcccaaga
      361 cgaaatgcct ccggcgcgtc gaccttgcgt ccggtgagaa ccaggtcggc ggcccgaccg
      421 gcaccgatgc gacgggccag caactgggta ccgccgccgc cgggtaccaa cccgacggtc
      481 acttctgtga ggccgaagac cgcagtctcg tcggcgacaa tcaggtcaca cgacagcgcg
      541 agttcgaacc cgccacccaa tgcgaagcca cccactgctg ctatcacggg catcgggagg
      601 tcgagaagcg ctgcgaacat ccccttgatg aacgggcgct gcgcaaggag ctgctcgttg
      661 ctgaatcccg cgcgttcctt caggtcagcg cctacgcaga aggctcgctc ctgcgacgac
      721 gacaagacga cggcacgcac cgccgcgtcc gcagcaagag cgcgcaacat cccgccgagg
      781 tgtgacgcaa ggtctgtcga tatcgcgttc agcgcgcccg gccggtcgag gatgagttcg
      841 acgatgtgtt ctgtgggccg ccacatcaag atgccagact gcccggcatc gggcgttgga
      901 acagagctca tacgttcgac agtacgccgc agttcagcgt tggcctggag cttcgtcggc
      961 ggcgttcggg tcagcagcaa caccgcgccc agcccgcccg ccgtccgcca cgctcatcgg
     1021 tccagttttc ccaaggttct ggtcaaccat gcggtagacc tcacgctgca cgtgctcgac
     1081 cttcggcacg tcggtcagcg agatctgtcc gttgtcaccc gccgactcga cgaccagggt
     1141 gccgctgcgc aacatgcgct cgagcacggt ttccgagaac gacacgtcgt tgatccggtt
     1201 cagcgggatg tcgcggccgt ggcgcgcgag gatgccgttg cggataatca gccggtcggt
     1261 cgtcagcacg tatttcgtcg tcagccagcg caggtacggc agtatcgtcc gccagatcac
     1321 caccacggcg gcgatggcga taaggatcca ccgctcgatc tgctggcccc cgcccttggg
     1381 gatcagcgcg acgccgaacc cggctaacag acaggccagc aagagcacca gaaccggcag
     1441 aaaaagccgg ccccagtgcg ggtgtaggtc gaggacaact tcttcgccgt ccgacaaatt
     1501 catcgccatg ccccgcatcg ttccatgccg gggcgaattg cgctcgccct ctcgaccgtc
     1561 gggcgacaaa atctccccat gactgcctcc gtgaccgttc gagcagcgac gaccgccgac
     1621 gctgccgcca tcgccgacat ctaccggccg tacgtgctgg agaccgcgat ctccttcgag
     1681 gagacgccgc caacagcagc tgatttcgag gcgcgcatgc tcgcaactcc gcgcctcccg
     1741 tggctggtcg ccgtccgcga aggcgcggtg ctcggctact gctacgcctc gcgccacgcc
     1801 gcccgggcct cgtaccgctg ggcggccgac gtctccgtct acgtcgcggg aggagagcgc
     1861 ggccgcggcg tgggtcgcct gctgtacagc gagctactgc cgctcgtgca aggactcggc
     1921 tacgtcagcg tgttcgcggg catcaccctc ccgaacgacg cgagcgtcgg cttccaccag
     1981 gcgttcggcc tcacgccagt gggcgtctat cgaaacgtcg ggttcaagca cagcaaatgg
     2041 ctggacgtcg gttggtggca gctcactttc accgatccgc ccgatcaacc agccgagcca
     2101 agagcgtggg taccgccagc tctcttgtga ctcaaaggcg aggcgacacg agcgaacgtt
     2161 attacggcat ttccggatac ccgttgtcg
//
LOCUS       DASWVF010000386         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_418097, whole genome shotgun sequence.
ACCESSION   DASWVF010000386 DASWVF010000000
VERSION     DASWVF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418097"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGD55_06975"
     CDS             complement(<1..373)
                     /locus_tag="VGD55_06975"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEY0870123.1"
                     /translation="MTAAGEPDDDPIEAYLDQALVSLPGSARQVRHTLAEIEAHLLDA
                     ATAAQADGLDEPTARALAVRQMGPIAGIAGRPSLTLRLTPALRRRVVLSALLIGGIGG
                     IAVGIGGVIGAVVNATLGDRAI"
     gene            complement(370..693)
                     /locus_tag="VGD55_06980"
     CDS             complement(370..693)
                     /locus_tag="VGD55_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0870124.1"
                     /translation="MRPDMLRGHLDALVLGALASGPAHGYAVIVALRDSTDGEFDLAE
                     GTVYPALHRLENQGFLVASVTEVQGRRRKVYELTASGRRELGEQRADWRRFSMLVTRV
                     IGATA"
     gene            985..1671
                     /locus_tag="VGD55_06985"
     CDS             985..1671
                     /locus_tag="VGD55_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870125.1"
                     /translation="MHNPTTHNEHAPLTIPACPSWCEQPAGHDYDMVTNSTGHLSRYH
                     EKRLAAVDLAAQLLPGDGHDITVGLFAEETAAGGVAECGPALLFLGAENPLLTSEDAR
                     AAAHALLEAAHSLDSLDPDWQAVRAAAHEIDPSPLTCPPWCVAPHDQPRYIAGGASAE
                     HWIWQGARDGIEVSVDVFDGDNGLDEPNITVSGRVEQLTADQADTLVALVTTAAARLR
                     EFGKLEASPS"
     gene            1668..2051
                     /locus_tag="VGD55_06990"
     CDS             1668..2051
                     /locus_tag="VGD55_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870126.1"
                     /translation="MINAPDDGDLEHLRAMTWAEPCPPWCNEHHEPLSALEIVVDMGI
                     EHVWWSRTIDMDPEDGVPRDLTVEVSATRCQDDWDPPVIFVLDGSGGGVEPMTTAATA
                     RRLADTLVAAADQLHLLIEWFENLR"
     gene            complement(2114..>2392)
                     /locus_tag="VGD55_06995"
     CDS             complement(2114..>2392)
                     /locus_tag="VGD55_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870127.1"
                     /translation="CERRRASAMAARDVADDARERRMRASSETSLTSARPTLPDVFVP
                     FDLAVIATGRHERTIRTWARRLKVRSVCDVRTRGVRVALADVVRANLS"
BASE COUNT          417 a          802 c          774 g          399 t
ORIGIN      
        1 cgatagctct gtcgccaagg gtggcgttca ccactgcgcc gatcacgccg ccaatgccga
       61 cggcgatccc gccgatccca ccgatcagca acgccgataa caccacacga cgccgcagcg
      121 caggagtcag gcgcagcgtg agcgacggcc ggccggcgat gcccgcgatc ggacccatct
      181 gccgtaccgc aagcgctcgt gccgtcggct cgtcgagccc atcagcctgc gccgcggttg
      241 cggcgtcgag taggtgtgcc tcgatttcgg ccaaggtgtg ccggacttgg cgcgcagatc
      301 cggggaggct gacgagcgcc tggtcgaggt aggcctcgat cgggtcgtcg tcaggttcgc
      361 ctgccgcggt catgcggtcg ccccgatgac ccttgtgacc agcatgctga accggcgcca
      421 gtcggcccgc tgctcgccta actctcgtcg tccggatgct gtgagctcgt acacctttcg
      481 tcgtcggccc tgcacttcgg tgacactcgc aacaaggaag ccttggttct cgaggcgatg
      541 taatgccggg tagacggttc cttcggccag gtcgaactcg ccatcggtac tgtcgcgcaa
      601 ggcaacgatg acggcgtacc cgtgcgccgg gccggatgcc aaagcaccaa gcacgagcgc
      661 atcaaggtgg ccgcgcaaca tgtcaggtct catacccagg cagtctaggc atagccagcc
      721 gaggtaagca agccttcgac gtctaggcct tcttggtggc caactattcg gccaccccca
      781 ctgacctgcg gcgacgtgct cacgcctcgt ctgacctgcg ctgatgctgt ttgcaagcgt
      841 tggccgacgt tgacctgatt cgagcgtctt gcggactacc tgcggaccaa cgatcgcgcg
      901 gctgagtgcg ccagtgcgcg gtccgcgcct cgatcacagg ctctaaccct gacatcaacc
      961 gactctccga aagggatgac atccatgcac aacccgacga cacacaacga acatgcaccg
     1021 ttgacgatcc ccgcgtgccc gagctggtgc gaacaaccag cgggccacga ctacgacatg
     1081 gtgaccaaca gcaccggtca cctgtcccgc taccacgaga agcgactagc tgcagtcgac
     1141 ctggcggcgc agctcctacc cggtgacggg cacgacatca ccgtcggcct cttcgccgag
     1201 gagacagctg ccggcggcgt cgccgagtgc ggcccggcgc tgctgttctt gggagcggag
     1261 aacccgctgc tgacgtccga ggacgcgcgt gcggccgcgc acgcgttgtt agaggccgcg
     1321 cacagcctgg actcgctcga ccccgactgg caagcggtgc gtgcagccgc gcacgagatc
     1381 gatccctcac cgttgacctg cccaccgtgg tgcgtcgctc cccacgatca gccgcgctac
     1441 atcgccggcg gcgcgagcgc cgagcactgg atctggcaag gagcccgcga cggcatcgag
     1501 gtcagcgttg atgtcttcga cggcgacaac ggcctcgatg agcccaacat caccgtttcg
     1561 ggaagggtcg agcaactcac ggccgaccaa gccgacaccc tggtggctct cgtcaccacg
     1621 gcggcagcgc gcctgcgaga gtttgggaaa ctcgaggcga gtccttcatg atcaatgcgc
     1681 ccgacgacgg ggacctggaa catctgcggg ctatgacgtg ggccgagccc tgcccgccgt
     1741 ggtgtaacga gcaccacgaa ccgctctccg ctctcgagat cgtcgtggac atgggtatcg
     1801 agcatgtgtg gtggtcgcgc acgatcgaca tggaccctga agacggggtt ccgcgcgatc
     1861 tcaccgtgga agtctccgca acgcgatgcc aggacgattg ggacccgccg gtgatcttcg
     1921 tgctcgacgg cagcggtggt ggcgttgagc cgatgacgac tgcggctacc gccaggcgtc
     1981 tggcggacac gctcgtggcg gccgcggatc aattgcacct gctcatcgaa tggttcgaaa
     2041 acctgaggta gatctggccg tgctttcgtg gttgcagagg gcgtcgcgtt cgaggtcgag
     2101 cgcggcgcct ctgctatgac aggttggcgc ggacgacgtc cgcgagtgcg acgcgaacgc
     2161 cgcgtgtcct gacgtcgcag actgagcgga ctttcagccg gcgtgcccag gtgcggatag
     2221 tgcgctcatg cctgccagtg gcgatgacgg ccaggtcgaa cggcacgaac acatcgggca
     2281 gcgtaggccg cgcactcgtg agcgaggttt cgctggaggc tctcatccgg cgctcgcgag
     2341 catcatcagc cacgtcgcgc gcggccatcg ccgaggctcg tcttcgttcg ca
//
LOCUS       DASWVF010000387         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_214255, whole genome shotgun sequence.
ACCESSION   DASWVF010000387 DASWVF010000000
VERSION     DASWVF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214255"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..767)
                     /locus_tag="VGD55_07000"
     CDS             complement(27..767)
                     /locus_tag="VGD55_07000"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870128.1"
                     /translation="MNPDADGAAFVTRYFEHVAADDMAGWPQSQAEEIAVGHRDFAAV
                     RPAGVPKVRVFASPTHPRHAVIEIVTDDMPFLVDSVTQELASKDVAIHLLVHPQFVVK
                     RDLHGRLLAVSGTLDAHDAPKDALVESWMHLEVAGLADQNAEENLRRDIVRVLQDVRD
                     AVEDWPRMRAHAARAADELHAEPPLAVPEAENNEAVEFLRWLADDHFTFLGYREYRLV
                     DDDGVDALAAVSGTGLGILRADKRGRGR"
     gene            complement(757..>1910)
                     /locus_tag="VGD55_07005"
     CDS             complement(757..>1910)
                     /locus_tag="VGD55_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0870129.1"
                     /translation="PLSPPGVWNRLDHRPLEGFVLAITPFNFTSIAGNLPTAPAIMGN
                     VVVWKPAPTSQLSSHYLMALLTEAGLPPGVISMVTGFGSAVSQVAVPHRDLAGIHFTG
                     STPVFQKLWRAVGENIASYRTYPRLVGETGGKDFVIAHPSADIDVLRTALVRGAFEYQ
                     GQKCSAASRAYIPRSVWRRLEGDFLAEVDALAMGDVSDFSNFMGAVIDDRAFARLHEA
                     IERARATSGLSVIAGGQTDDSEGWFVRPTVVQGDDPTDEVFVREYFGPLLAVHVFDDD
                     RYGAVVAQAADASPYALTGAVIAQDREAIADATQNLRYAAGNFYVNDKPTGAVVGQQP
                     FGGGRASGTNDKAGSAQNLLRWTNTRAIKETFVPAKTSQYPHMGPADEP"
BASE COUNT          323 a          625 c          641 g          321 t
ORIGIN      
        1 cgggcgaacc tcctcgggaa ggtccttcag cggccgcggc ctcgcttgtc ggcccgaagg
       61 atgccgagcc cggtaccaga cacggcagca agtgcatcga ccccgtcgtc gtcgaccagc
      121 cggtactcgc ggtagccaag gaacgtgaag tggtcgtcgg ccagccaccg caggaactcg
      181 acggcctcgt tgttctcggc ttccggcacc gcgagcggcg gttcggcgtg cagttcatcg
      241 gccgcgcgag cagcgtgcgc ccgcatgcgg ggccagtctt cgacggcgtc ccggacgtct
      301 tggagcaccc gcacgatgtc gcgacgcagg ttctcctcgg cgttctggtc ggcgaggccc
      361 gccacctcga ggtgcatcca cgactcgacg agcgcatctt tcggtgcgtc gtgggcgtca
      421 agcgtgccgg agacggcgag caaacgtccg tgcaggtcac gcttgaccac gaattgaggg
      481 tgcacaagga ggtgaatcgc gacgtccttg ctcgccagtt cttgggtgac cgaatcgaca
      541 aggaacggca tgtcgtcggt gacgatctcg atgaccgcgt ggcgcgggtg cgtcggtgac
      601 gcaaagaccc gcaccttcgg caccccggcg ggtcgcaccg cagcgaagtc gcgatggccg
      661 acggcgatct cttctgcttg cgactgtggc cagcccgcca tgtcatcggc ggcgacgtgc
      721 tcgaagtacc gtgtgacgaa cgcggcgcca tcggcgtcag ggttcatcgg ccggccccat
      781 gtgcgggtac tgcgacgtct tcgccggcac gaatgtttcc tttatggccc gcgtattggt
      841 ccagcgcagg aggttctggg cagagcctgc cttgtcgttc gtgccgctcg cgcgaccacc
      901 gccgaacggc tgctggccca cgaccgcgcc ggtcggcttg tcgttcacgt agaagttgcc
      961 ggccgcgtag cgcaggttct gcgtggcatc ggcgattgct tcgcgatctt gtgcgatcac
     1021 ggcacctgtc aatgcgtagg gcgacgcgtc agcagcctgc gctacgacgg cgccgtaccg
     1081 gtcgtcgtcg aaaacgtgca ccgcaagaag ggggccgaaa tactcgcgca cgaagacctc
     1141 gtcggtcggg tcgtcgcctt gcacgacggt cggtcgcacg aaccagcctt cgctgtcgtc
     1201 ggtctgccct cccgcgatga ccgataaccc ggacgtcgcg cgagcccgct cgatcgcttc
     1261 atgcaggcga gcaaatgcac gatcgtcgat cacggcgccc atgaagttcg agaagtcgct
     1321 gacgtcaccc atcgcaagcg cgtcgacctc ggccaggaag tcgccttcga gccggcgcca
     1381 caccgaacgc gggatgtagg cgcgcgacgc ggccgagcat ttctggccct gatactcgaa
     1441 agcgcctcgt accaaggcag ttctcagcac atcgatgtcg gccgaagggt gagcgatgac
     1501 gaaatccttg ccgccggttt cgcccacgag acgcggatag gtgcggtaac tcgcgatgtt
     1561 ctcgccgacc gcccgccaca gcttctggaa aaccggcgtc gacccggtga agtggatgcc
     1621 ggcgaggtca cggtgcggca cagcgacctg cgagaccgca gatccgaaac cggtcaccat
     1681 cgagatgacg cccggcggga gtcccgcttc ggtgagcaag gccatcagat agtgcgacga
     1741 cagctgcgat gtcggtgcgg gtttccagac cacaacgttg cccatgatcg cgggcgccgt
     1801 cggcaggttg cccgcgatcg aggtgaagtt gaacggcgtg atggccagca cgaatccctc
     1861 gagcgggcgg tggtcgagcc ggttccagac ccccggcggt gacagcggct
//
LOCUS       DASWVF010000388         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_188745, whole genome shotgun sequence.
ACCESSION   DASWVF010000388 DASWVF010000000
VERSION     DASWVF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188745"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VGD55_07010"
     CDS             complement(<1..307)
                     /locus_tag="VGD55_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870130.1"
                     /translation="MEMTADGESGMFAGWQKTGWSKAGWTKEEWKNAARQRAAEFKAA
                     ARDAGCDRAAFRKLMRDRMRDHWAAAATDTQDTEQSWAEADAPRGERWGGPGHQHRGR
                     "
     gene            462..965
                     /locus_tag="VGD55_07015"
     CDS             462..965
                     /locus_tag="VGD55_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0870131.1"
                     /translation="MPTDGEITFADHAGRLYARRYGMAPMVGRLMGYLAICDPREQSI
                     GELAEALLASRSAIAGAVTVLETLGLISRSRAAGERMDRIRIDLSAPRSMGFDVTEYQ
                     EQGDLAREGLALLDGESLERRAVVLEWAAFADFLVARLPVLEQDWKAHRQALRAAGEL
                     PDNPPSR"
     gene            980..1951
                     /locus_tag="VGD55_07020"
     CDS             980..1951
                     /locus_tag="VGD55_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870132.1"
                     /translation="MTSQNVSAISARAVSKSYGEHVVLDGVDLTVAEGTIFSLLGPNG
                     AGKTTMVRILSTLIRADAGEIRIGGHDVVTDPDAVRALIGVTGQFSAVDNYLTGAENL
                     QLMADLRHFGRDAGRRRVAELLERFELSDAAEKPLSTYSGGMRRRLDLAMTLVGNPRV
                     IFLDEPTTGLDPRSRRTMWQIIRELATDGVTVLLTTQYLEEADRLADQIAVLDHGRVV
                     AEGSPAELKRRIPGGHIQLHFGEAADLRSAANLFPRASADLDQLVLQVPDSDGVASLR
                     RVLDDLDAASIKVESLSIHSPDLDDVFFAVTGRTSSEQAREAEEALR"
BASE COUNT          330 a          659 c          648 g          377 t
ORIGIN      
        1 cgcgaccgcg gtgttggtgc ccgggaccgc cccagcgctc gccgcgcgga gcatctgctt
       61 ccgcccacga ctgctcggtg tcctgcgtgt ctgtcgctgc ggctgcccag tggtctcgca
      121 tgcggtcgcg catcagcttg cggaaagccg cacggtcgca gcccgcgtcg cgagccgccg
      181 ccttgaactc ggcagccctt tggcgggccg cgttcttcca ctcttctttc gtccagccgg
      241 ccttgctcca gccggtcttc tgccagccgg caaacatgcc ggactcgccg tcagctgtca
      301 tctccatgat gtcctcctgc gttggtacgt gtgactatca cgatatatcg cgtacgagtc
      361 ttgtcaagat ggtacaagga ggtgatcgtg gcagcggccc cacgtctcca tcagatcaca
      421 ctgatttgtg ttatctcagt tacatgtgta tcttccgtgc tgtgccaacg gatggagaga
      481 tcactttcgc cgaccacgca ggtcggctct atgcgcgccg ctacggcatg gcgccgatgg
      541 tgggccggct catgggctat ctggcgatct gtgatccgcg ggagcagagc atcggggagt
      601 tggccgaggc gttgctggcg agtcgcagcg cgatcgccgg tgcggtcacc gtgttggaga
      661 cgctcggctt gatcagtcgc tcgcgcgccg cgggtgagcg gatggaccgg atccgcatcg
      721 acctatccgc gccccggtcg atggggttcg acgtgaccga gtaccaagag cagggcgatc
      781 tcgcgcgcga aggcttggcg ctgctcgatg gcgaatcgct cgaacgacgt gcggtcgtgc
      841 tggagtgggc cgcgttcgcg gacttcttgg tcgcgcgact gccggttctc gagcaggact
      901 ggaaagccca ccggcaggcg ttgcgggcgg ctggcgaact ccccgacaac ccgccgtccc
      961 gatgaaagcg agaagtgtga tgacgagcca gaacgtgtcg gcgatcagcg cccgcgcggt
     1021 cagcaagtcc tatggcgagc acgtggtgct cgacggggtc gacctcacgg ttgccgaggg
     1081 cacgatcttc tcgctgctcg ggccgaacgg ggccggcaag acgacgatgg tgcggatcct
     1141 gtcgacgttg atccgtgccg acgcgggcga gattcgtatc ggtggtcacg acgttgtgac
     1201 cgatcccgac gcagtgcgcg cattgatcgg cgtgacgggt cagttttctg cagtggacaa
     1261 ctacctcacc ggcgcggaga acctacaact catggccgac cttcgccact tcggtcgcga
     1321 tgccggacgc cgtcgggtcg ccgaactact cgaacgcttc gagctcagcg acgcagccga
     1381 aaagccgctg tcgacctatt caggaggcat gcggcgccgc ctcgacctgg cgatgacgtt
     1441 ggtcgggaac cccagagtga tcttcctcga cgaaccgacc accgggctcg atccgcgcag
     1501 ccgtcgcacc atgtggcaga tcatccgcga gctcgcgacc gacggcgtca ccgtcttgct
     1561 aacgacgcag tacctcgagg aagcagaccg acttgccgat cagatcgcgg tgctcgatca
     1621 cggccgcgtc gtcgccgaag gcagcccggc tgagctaaag cgccgtatcc ccggtggaca
     1681 catccagctg cactttggcg aagcggccga cctgcgatct gcggcgaacc tgttcccccg
     1741 cgcctcagct gaccttgacc agctggtcct ccaggtgccc gacagcgatg gtgtcgcctc
     1801 gttacgtcgg gtcctcgacg atctcgacgc ggccagcatc aaggtcgaga gcctgtcgat
     1861 ccattccccc gacctcgatg acgtgttctt cgccgtcacc gggcgcacca gctccgagca
     1921 ggcccgtgaa gcagaggaag cgttgcgatg agttcgctca cttttaccgt cgccgattcg
     1981 aggacgatgt tgcgccgcaa cctcaagcac caag
//
LOCUS       DASWVF010000389         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_112187, whole genome shotgun sequence.
ACCESSION   DASWVF010000389 DASWVF010000000
VERSION     DASWVF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112187"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VGD55_07025"
     CDS             <1..460
                     /locus_tag="VGD55_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0870133.1"
                     /translation="KEIMKAPVYTVGLDMPTADVAKLLVQHRISAVPVLDATGAVVGL
                     VSEHDLISRDGPTAADVMSPGIVSVTEDTDVDDVRHLLVDRRIRRVPVMSGGQLVGIV
                     SRADIVALIAMEWVCEVCGTQARGEHPPASCPTCAADTVRFVHLQQPPGT"
     gene            544..1662
                     /gene="pdhA"
                     /locus_tag="VGD55_07030"
     CDS             544..1662
                     /gene="pdhA"
                     /locus_tag="VGD55_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY0870134.1"
                     /translation="MPPAQQKTADRAPAKAGNKRAAKPANTGTAFPGEPVERLRDYYR
                     LMQLIRRFEERTAEMYQRAKIGGYCHLNLGEEATVVGAMDAMHPSDYLFTTYREHGYA
                     IARGIDATRVMAELFGKTTGVSHGWGGSMHLYDVEKRLLGGYAIVGGQIPPATGAALA
                     IAYRGPGGPDTEAVVCQLGDATTNIGAWHEALNLAAIWKLPIVYFIVNNHLGMGTTVE
                     RASGEPDLYKRGSAFRIPGERVDGNDVIAVRDAMSQALRLAREERQPTIVEATSWRLR
                     GHSVVDPATYRTKEEMQRLHDLDPLTAFRRQLIDAKVLSTEGAAAIDADVEIEVNACI
                     AFADESPDPSPDQLFAHAYATPVANAPRELPGDPLHVL"
     gene            1678..2673
                     /locus_tag="VGD55_07035"
     CDS             1678..2673
                     /locus_tag="VGD55_07035"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0870135.1"
                     /translation="MALITYRQALHDTLRSELLRDPNVFLMGEEIGIFEGSYKITAGL
                     LTEFGPERVRDTPISEEGFVGAAIGAAMLGLRPVVEIMTINFSIVAMDQIVNHAAKIH
                     TMFGGQSTVPMVIRTPGGGGQQLAATHSQNLEVWYAHVPGLKVVMPSTPADTKGLLAA
                     SIRDNDPVMFIEHLALYNTKGEVPDGDYVTEIGKAAITKAGTDITIVSYSRMAAVALD
                     VARRLEEDEGISAEVVDLRSLRPLDRATILQSVRKTGKAVVLEEDWLSYGVGAEIAAT
                     IQEGAFDYLDAPVRRVAAAEIPLPYAKPLELAALPDATALLKVIHETLDATRFRG"
     gene            2697..4058
                     /locus_tag="VGD55_07040"
     CDS             2697..4058
                     /locus_tag="VGD55_07040"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEY0870136.1"
                     /translation="MPQVFMPRLSDTMTEGTVSTWTKGVGDRIEKGDVIAEIETDKAT
                     MELEAYDAGILEQILVGVGTTVPIGEVIAMIGDGSGTAAPSAPASSAAPATPVAAAAP
                     EPAPESAPAAEAAPATAPASAAAAAAPTPAALAPAAAPNGKHPLASPLARSIARDAGL
                     DLTTVTGSGPGGRIVRADVQNAIAALAGSGSAGTSGSKPGPSTSTSTLSTAPAVPLQT
                     GPDDEEIPLNNVRKITARRLVESMQQAPHFYVTSVVAADELVAARARINEGLAALAAD
                     GAVTKISVNDLVVKAVAHALRAHPEVNVSFGGDKLIRHRRVHVGVAVALPDGLIVPVI
                     RDADTKTVTQISAETRDLATRARAGKLKPDEFTGSTFSVSNLGMFGVDQFTAVINPPE
                     AAILAVGATKQEPVVRDGQIVVGYTMAITLSVDHRALDGATGAKFLADLTALLENPLA
                     TLA"
     gene            complement(4244..4606)
                     /locus_tag="VGD55_07045"
     CDS             complement(4244..4606)
                     /locus_tag="VGD55_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870137.1"
                     /translation="MAVIVLVTFSTGACSPRQHYAENNTYAALTGDKGEIKGALAAVD
                     GSAGSSSEPVQGAVDVYTLGGHEVANISGVSAWNFELEPGIYTLQATAIDLVCPPIKA
                     TVDKGTVRVIPPLVCTTK"
BASE COUNT          842 a         1623 c         1513 g          791 t
ORIGIN      
        1 caaagagatc atgaaggcgc cggtctacac ggtcggcctc gacatgccta cggccgacgt
       61 cgcgaagctc ctggtccagc accgcatcag cgcggtccct gtgctcgacg ccaccggagc
      121 cgtcgtcggt cttgtgagcg agcacgatct catctcccgc gacggcccta ccgcagcaga
      181 cgtcatgtcg ccgggcatcg tcagcgtgac cgaagatacc gacgtcgacg acgtccgcca
      241 tctcctcgtc gaccggcgca tccgccgggt cccggtgatg tcgggcggcc agttggtggg
      301 catcgtcagc cgggccgaca tcgtggcgct gatcgccatg gagtgggtct gcgaggtctg
      361 cggcacacag gcgcgcggtg agcaccctcc ggcgtcctgc ccgacatgcg ccgccgacac
      421 cgtgcggttc gtccatctcc agcagccgcc gggcacctga gccgatccgc tgcgcccgca
      481 gcaccgacca gccgacgccc agccaacgcc cagtcgatgc ccatacgacc ccaaggagac
      541 gccgtgccgc ccgcacagca gaagaccgca gaccgcgcgc cagccaaggc cgggaacaag
      601 cgcgcagcga agcctgcaaa caccgggacc gcattccctg gcgagcccgt cgagcgcctg
      661 cgcgactact accggttgat gcagctcatc cgccgtttcg aagagcgcac ggccgagatg
      721 taccagcgcg ccaagatcgg tggctactgc cacctcaacc tcggcgaaga ggcgaccgtg
      781 gtcggggcga tggatgcgat gcacccgtcg gactacctgt tcacgacgta tcgcgagcac
      841 gggtacgcca tcgcccgcgg catcgacgcc actcgcgtga tggccgagct gtttgggaag
      901 acgaccgggg tatcgcacgg gtggggcggc tcgatgcatc tctacgacgt cgagaagcga
      961 ctgctcggcg gttatgcgat cgtcggcggt cagattccgc ctgccaccgg agccgcgctc
     1021 gcaatcgcct accggggtcc tggcggtcct gacaccgaag cggtcgtgtg ccagctcgga
     1081 gatgcgacaa cgaatatcgg cgcctggcat gaagcactca acctggccgc gatctggaag
     1141 ctgccgatcg tctacttcat cgtcaacaac cacctcggca tggggacgac ggtcgagcgg
     1201 gcatcgggtg agcctgactt gtacaagcgc ggatccgcgt ttcgcatccc cggcgaacgg
     1261 gtcgacggca acgacgtgat cgccgtgcgc gacgcgatgt cgcaggcgtt gcggctcgct
     1321 cgcgaggagc ggcagccgac catcgtcgag gccacgagct ggcggctgcg cggccactcc
     1381 gtcgtcgacc cagctacgta ccgcaccaag gaagagatgc agcggctgca cgatctcgac
     1441 ccgctgacgg cgtttcggcg tcagctgatc gacgcgaagg tattgagcac cgaaggagca
     1501 gcggcgatcg acgccgacgt cgagatcgag gtcaacgcgt gtatcgcgtt cgccgacgag
     1561 tcacctgacc caagccctga ccagctcttc gcgcacgcgt atgcgactcc ggtcgcgaac
     1621 gcgccacgcg aattgcccgg cgaccccctt cacgtcctgt agcgaaaggc gattcccgtg
     1681 gccctcatca cttatcgcca ggcgctgcac gacacgttgc gctctgagct gcttcgcgat
     1741 cccaacgtct tcctcatggg cgaagagatc ggtatcttcg aaggttctta caagatcacg
     1801 gcgggcctgc tgaccgagtt cggtcccgag cgggtgcgtg acaccccgat ctccgaagag
     1861 ggattcgtcg gcgctgcgat tggtgccgcg atgttgggcc tgcggccggt cgtcgagatc
     1921 atgacgatca acttctcgat cgtcgcgatg gaccagatcg tcaaccacgc ggcgaaaatc
     1981 cacaccatgt tcggcggtca atcaaccgtt ccgatggtga tccggacgcc gggtggtggc
     2041 ggtcagcagc tcgcagcgac gcactcacaa aacctcgagg tctggtacgc gcatgtccct
     2101 ggcctcaaag tcgtcatgcc gtcgacgccc gccgacacga agggcctgct cgcagcatcg
     2161 atccgcgaca acgacccggt gatgttcatc gagcaccttg ccctttacaa cacgaagggc
     2221 gaggtaccgg acggcgacta tgtgaccgag atcggcaagg cggccatcac caaggcgggc
     2281 accgacatca cgatcgtgtc gtactcgcgg atggccgcgg tcgcactcga cgtcgcgcgc
     2341 cgactcgaag aggacgaggg catctcggcc gaggtcgtcg accttcgatc gctacggcca
     2401 cttgatcgcg cgacgatcct ccagtcggtc cgcaagaccg gaaaggcggt cgtgctcgag
     2461 gaggattggc tgtcgtacgg cgtcggcgcc gagatcgcag ccacgattca agaaggtgcg
     2521 ttcgactacc tcgacgcacc ggtgcgccgg gtcgcggccg ccgagatccc cttgccttat
     2581 gcgaagccgc tggagcttgc cgcgctgccc gacgcgacgg ccttgctcaa ggtgatccac
     2641 gaaaccctcg acgccacccg cttccgcggc tgagctcacg agcctaggag ttttcgatgc
     2701 cgcaggtctt catgccacgt ctttccgaca ccatgacgga aggaacagtg agcacctgga
     2761 cgaagggcgt aggtgaccgc atcgagaagg gcgacgtgat cgccgagatc gagaccgaca
     2821 aggccacgat ggaactcgag gcctacgacg ccggcatcct ggaacagatt ctcgtaggtg
     2881 tcggcaccac cgtgccgatc ggcgaagtca tcgcgatgat cggtgacggc tcaggtactg
     2941 ctgcaccatc ggcacctgct tcgtcggcag cacccgcgac ccctgttgcc gcggccgcgc
     3001 cggagcccgc gccggaatct gcgcccgccg ccgaagccgc ccctgcaacc gcgcctgcat
     3061 ctgccgcagc cgccgcggcg ccgacaccgg ctgcactggc gcccgccgct gcgccgaacg
     3121 gcaagcatcc tcttgcctcc cctttggccc ggtcgatcgc acgcgacgct ggtctcgacc
     3181 tcacgacggt caccggctca ggacctggcg ggcggatcgt gcgggccgat gtgcagaacg
     3241 cgatcgccgc actcgcgggt tctggctcag caggaacctc ggggtcgaag cccggcccct
     3301 cgacgtccac ctcgacgctt tcaacggcgc ccgcggttcc cctgcagaca gggcccgacg
     3361 acgaggagat cccgctcaac aacgtgcgca agatcaccgc tcgccggctg gtcgagagca
     3421 tgcagcaggc accgcatttc tatgtgacca gcgtcgtcgc ggccgacgaa ctcgtggccg
     3481 cgcgggcccg catcaacgaa ggcctcgcgg ctctcgcggc cgacggggcg gtgaccaaga
     3541 tcagtgtcaa cgacctcgtg gtgaaggcgg tcgcgcacgc actgcgcgcc caccctgagg
     3601 tgaacgtgtc gttcggtggc gacaagctga tccggcaccg cagggtgcac gtcggtgtcg
     3661 cggtcgcact gcctgatggc ctgatcgtgc cggtgatccg tgatgccgac acgaagacgg
     3721 tgacgcagat ctcggccgag acccgtgatc tcgcgacccg tgcccgcgcc ggcaagctca
     3781 agcccgacga gttcaccggc agcacgttca gcgtcagcaa cctcgggatg ttcggcgtcg
     3841 accagttcac ggcggtcatc aacccgccgg aagcggcgat cctcgctgtc ggcgcgacca
     3901 agcaggagcc ggtcgtacgc gacgggcaga tcgtggtcgg ctacacgatg gcgatcacgt
     3961 tgtcggtcga ccatcgcgcc ctcgacggcg cgaccggcgc caagttcctc gccgatctca
     4021 ccgcattgct ggagaacccg ctcgcgaccc tggcgtagga acggccggat cagtgccgca
     4081 tggtttcttg ccaccacctg gccgcaacga aagcactact ttgccgatga gcggatgtcc
     4141 gctggggcgc ttcttccgtt cccaggcggg attcagtcgg cctcgatcaa actcgtggag
     4201 ggtgtacttg gcgttgacgg aatgcagtcc ttcgttgggc gtctcacttt gtggtgcaga
     4261 cgagcggcgg gatgacccga accgttccct tgtcgactgt tgccttgatc ggcgggcaaa
     4321 ctagatcgat cgcggtggct tgcagcgtgt agatacccgg ctctagttcg aagttccacg
     4381 cggacacccc cgagatgttg gcgacctcat ggccccccag cgtgtagacg tccactgcac
     4441 cctgaacggg ctcggaactg gagccagccg atccatcgac tgccgctagc gctcccttga
     4501 tttcgccctt gtcccctgtc agggcagcgt acgtattgtt ctcggcgtag tgctggcgtg
     4561 ggctgcacgc accggtgctg aatgtgacca acactatgac tgccaccgcg gcaatgcggg
     4621 ccctgtacac caatgtctcc tttcgagacg attgtctggt catcgtaggc gactatccgg
     4681 ccgcctcatc tggctcgatg acgcgtgccg tcatggggtc gtcggacctt gtaactcacg
     4741 tcccgtgtcg aagaacgcgt tgaggtact
//
LOCUS       DASWVF010000390         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_239644, whole genome shotgun sequence.
ACCESSION   DASWVF010000390 DASWVF010000000
VERSION     DASWVF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239644"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VGD55_07050"
     CDS             complement(<1..1192)
                     /locus_tag="VGD55_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HEY0870138.1"
                     /translation="MNPHLLSASVSRSRRTFPSVLFDEAHSEAWSIRAETVAEMNPRH
                     PGDAGYLKAATALRERGMVIDAHTAGLLTADVLADRDVVVLAHPSDGTWERVTGIGSA
                     TLSPDEIDTLETFVRDGGGLVVMAECEQDKYGNNLSGLLERFGVRPLNVTVQDTEHNH
                     QDVVAWVRAQLPPQRGKADLLAQVSAACFYRSGVLSITNPDAVVLAKTSSSADPPDQP
                     LAVALQFGLGRLVVFADSDLFGDDSIGDYDHVTLWSNAITWAASGLSAETSTVAEPSW
                     LLQHPGWVALKDSVETIRGLQAKDGSLDVETHGISARDAATAELDTCVSAVEALRPHF
                     EHDSDYLDALILDVQRWRDGGFAVPDFLDSLMAFHPELHRVDGLEHLVVFPMYTQNGN
                     PDRNL"
     gene            complement(1245..1601)
                     /locus_tag="VGD55_07055"
     CDS             complement(1245..1601)
                     /locus_tag="VGD55_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870139.1"
                     /translation="MSRVQRCVAFPQRARGMSDGVLEAPNRRTTDPDAVMPLMSYSRW
                     KKTDGTFGPVGRVVATLLLIVPLPVFALGVATGIGIIGAGLYVVIVMPWALRDIWKRG
                     ATHVQPPLPARGRPQF"
     gene            1717..>2382
                     /locus_tag="VGD55_07060"
     CDS             1717..>2382
                     /locus_tag="VGD55_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0870140.1"
                     /translation="MDTVDPTGASDLVAYLDHAATTAMVPVAVDAMTRQLGLVGNPSS
                     LHRAGRAARRVVEEARETVAQALHARPSEVVFTSGGTEADNLAVKGIFWARRAADPRR
                     VRILASAVEHHAVLDCVEWLARHEGAEPVWLDVDVLGRVQPETVRSAIEADPDSVALV
                     TVMWANNEVGTVQPIAEIVAIAHAYGIPVHSDAVQAVGQLPVDFAASGLDAMSVSGHK
                     VGGP"
BASE COUNT          388 a          792 c          819 g          383 t
ORIGIN      
        1 cgagattgcg gtcggggttg ccgttctggg tgtacatcgg gaacacgacc aggtgctcga
       61 gcccatcgac ccggtgcagt tcggggtgaa aggccatcag ggagtcgagg aagtcgggca
      121 cggcgaagcc gccgtcgcgc caccgctgca catcgagaat caacgcgtcg agatagtcgg
      181 agtcgtgctc gaagtgcggg cgcagcgctt cgaccgccga aacgcaggtg tcgagttctg
      241 cagtggcggc atcgcgtgcc gagatcccgt gtgtctcgac atcgagagac ccgtccttgg
      301 cctgaaggcc gcggatcgtt tccaccgaat ccttcagcgc aacccagccg ggatgctgca
      361 acaaccagct cggctcggcc acggtagatg tctcagctga caagcctgag gctgcccagg
      421 tgatcgcatt gctccacagc gtgacgtggt cgtagtcgcc gatcgaatcg tcgccgaaca
      481 ggtcggaatc ggcgaacacc acgaggcggc cgagcccgaa ctggagcgcc accgcgagcg
      541 gttgatcagg tgggtctgct gagcttgacg ttttcgccag caccacggcg tccggattag
      601 tgattgacag cacgcccgac cggtagaagc aggcggcaga cacttgcgcg aggaggtcgg
      661 cctttccgcg ctgcggcggc aactgcgcgc ggacccaggc cacgacgtcc tgatggttgt
      721 gctcggtgtc ttgaacggtc acgttcaacg gccgcacgcc gaaccgttcg agcagaccgc
      781 tcaggttgtt gccgtatttg tcttgctcgc attcggccat cacgacgagc ccgccgccgt
      841 cgcggacgaa cgtctccagc gtgtcgatct cgtcaggcga caacgtcgca gagccgatgc
      901 cggtgacgcg ctcccaggtg ccgtcggacg gatgcgcgag gacgacgacg tcgcgatcgg
      961 cgagcacgtc agccgtcagc aatcccgcgg tgtgcgcgtc gatgaccatg ccgcgttcgc
     1021 gtaaagcggt cgctgctttg aggtaccccg cgtcgccagg gtggcgtgga ttcatctcgg
     1081 cgacggtctc agcccggatg ctccaggctt cgctgtgcgc ctcgtcgaac agcacgctcg
     1141 gaaacgtgcg tcgcgagcgc gagacggatg ccgagagcag gtgcgggttc accccatgca
     1201 gcctaccggc cgggcctgcg ccggcccggg tgagcgcgag cggctcagaa ctgagggcgt
     1261 ccacgcgcgg gcaacggcgg ctggacgtgc gtcgcgccgc gcttccagat gtcgcgcaac
     1321 gcccacggca tgacgatgac gacgtagagc ccggcgccga tgatgccgat cccggttgcc
     1381 accccgagag cgaaaaccgg aagcggcacg atcagcagga gggtcgcgac gactcggccg
     1441 accggtccga acgtgccgtc ggtcttcttc cagcgcgagt agctcataag gggcataacg
     1501 gcgtcagggt cggtcgttcg acggttcggg gcctcgagca cgccatcgct catgccgcgt
     1561 gcgcgctgcg ggaaggccac gcaacgctgg acacggctca tcggcgcaat cccgcacgcg
     1621 cacgggtgtt gcgcaggcag ggtctgctcg gcggccacgg tctgtcatcg gcatcttggg
     1681 cgcgctcgtt gagcccggac ggataccctg gaatcgatgg acaccgtcga cccgaccggc
     1741 gcctctgacc tggtcgctta ccttgaccac gccgccacca ctgcgatggt gcccgtggcg
     1801 gtcgacgcta tgacccggca gctcggactg gtcggcaacc cgtcgtcgtt gcaccgggct
     1861 ggccgcgctg cgcgccgggt cgtcgaggag gcccgcgaga ccgtcgccca agccctgcat
     1921 gcccgcccga gcgaggtcgt cttcacctct ggcggcactg aggccgacaa cctcgcggtc
     1981 aagggaatct tctgggcgcg gcgcgccgct gaccctcgtc gcgtccggat cctggccagc
     2041 gcggtggagc atcacgcggt cctcgattgc gtcgaatggc tcgctcgcca tgagggcgca
     2101 gaacccgttt ggctggacgt cgatgtgctg gggcgggtgc agccggagac cgtccgttcc
     2161 gcgatcgaag ccgaccccga ttccgtagcc ctggtcaccg tgatgtgggc caacaatgag
     2221 gtcggcaccg tgcaaccgat cgccgagatc gtcgccatcg cccacgcgta cggcatcccc
     2281 gtgcactccg acgcggtgca ggcggtcggc cagctgcccg tcgacttcgc ggcgagcggt
     2341 ctcgacgcga tgagcgtttc cggccacaag gtcggcgggc cg
//
LOCUS       DASWVF010000391         5376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_494394, whole genome shotgun sequence.
ACCESSION   DASWVF010000391 DASWVF010000000
VERSION     DASWVF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494394"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VGD55_07065"
     CDS             complement(<1..265)
                     /locus_tag="VGD55_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0870141.1"
                     /translation="MFGLDIGGSGIKGAPVDLARGELTAERVRIVTPPGAKVRDVAKT
                     AGEVVKGFGYKGLVGAAFPAVIVDGRALTASNVDTGWIGTDVSG"
     gene            complement(274..1386)
                     /locus_tag="VGD55_07070"
     CDS             complement(274..1386)
                     /locus_tag="VGD55_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0870142.1"
                     /translation="MADAQGSGGTRRPRKTTTIGKTKAPTGRVAFPGISSRAYEHPAD
                     RAALVTLRSVPGFDVLLKGMFGFFSERRLRMMYLASAVRVDERQFSGIYAIYQDAARI
                     LDIEKLPELYINQDPRVNAWALGLDNPFIVVSTGTVDLLDDDELRVVLGHELGHVLSG
                     HALYSSVLFALMRITGVSTIIPLGGLGLRAVITALKEWYRKAELSCDRAGLLVSQDPG
                     AVTRVNMKMAGGARVEDMDLQAFLAQAEEYESTGDAREGVIRILNILNSTHPFAVLRA
                     LEIKRWVASGEYERILAGTYPNRADDPRASFVDEVKAAAASYKQSFDRSPDPLMKFLR
                     DVGGGTVNLGGWVADQVRKMAGGKDPNDGSDDSSEG"
     gene            complement(1397..1537)
                     /locus_tag="VGD55_07075"
     CDS             complement(1397..1537)
                     /locus_tag="VGD55_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870143.1"
                     /translation="MNVGSPAQRKAREALFLVVQAQTAPARAATLERWSATAAARGLA
                     QA"
     gene            complement(1611..2489)
                     /locus_tag="VGD55_07080"
     CDS             complement(1611..2489)
                     /locus_tag="VGD55_07080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0870144.1"
                     /translation="MTEVAGHPLVEAARRLAAELLEPQAAIVDASVVPRSHLQALADA
                     GLLGLLAPAEVGGADASAAVFREVNELLAGADAATWFVQAQHHSVVRMLVSTPSPAAP
                     QYLARLASGELIAGIAFSHLRRFPDRPVVARPVDGGWRLDGVAPWYTGWGLNDVAFLS
                     GVTDEGEVIFGAVPAREGGGLSVRSALRTAALDAACTAVLGLDGVFVADHDVALRESH
                     AQWLAGDTLVANANAAVFGVAASAVELLRTAGVARDEPAATHGGDLRRAPGRRPHALL
                     CTSRYCSAGRRHRRAP"
     gene            2588..5029
                     /locus_tag="VGD55_07085"
     CDS             2588..5029
                     /locus_tag="VGD55_07085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY0870145.1"
                     /translation="MARKSAATPPPPPGVEQIVDIDVSEEMRGSFLEYAYSVIYSRAL
                     PDARDGLKPVHRRILFQMAAMGLRPDRPHVKCARVVGDVMGKLHPHGDTAIYDALVRM
                     AQPFSMRVPLVDGHGNFGSLGGDDPPAAYRYTECRLESAAELMTDGLSEEVVDFGPNY
                     DGQEEEPAVLPAAFPNLLVNGATGIAVGMATSMAPHNLVEVIAAARHLIKKPDATLDD
                     LMRFVPGPDLPTGGKIVGLDGVRDAYETGRGMFRIRATAKVTDVRPRKRGIVVTELPY
                     GIGPEKVIGKIKDLVQSRKLQGIADVRDLTDREHGLQLVIEIKNGFNAEAILDQLYKL
                     TPMEDTFGVNNVALVDGQPLTLGLKELLEVYVDHRFDVVRRRTTYRRRRREEQLHLVQ
                     GRLIAIVDIDEVIAVIRSSDDTAAARTRLMEVFDLSEIQANDILELPLRRLTKFSRLE
                     LEKERDQLSREITELTAILESDELLRTVVSDELGDVAKKFGTPRRTVLLESAGVPSAA
                     VPLEVTDDPCVVLLSATGLLARAQPTDDAPVTEGRTRHDVIASKVPSTARGEVGVVTS
                     FGRMIRLSVLELPALPPTVGAPTLAGGAPVHEFLELEKTENVLALTTLLPTSHGVALG
                     TASGVVKRVLPDYPSNKDSFELIGLKDGDTVVGAVELTSGDEELVFITSDAQLLRFEA
                     ASVRPQGRSAGGMAGVRLSSGAHVVFFGAVPVETDAVVVTIAGSSAALPGTDAGAAKV
                     APYSEYPPKGRATGGVRCHRFLRGEDTLLVGWAGAIPAKATASSGVAIELPAPNGRRD
                     GSGTPLASPVLAIGN"
     gene            5108..>5376
                     /locus_tag="VGD55_07090"
     CDS             5108..>5376
                     /locus_tag="VGD55_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870146.1"
                     /translation="MSEGAPQLHVSSEPTWVVVGLDNGGTCNNATVLTDSGEFLVDHL
                     VENPSRVNDGPEIAVASLADSLEAIIDLTGVPRMHVRAVGLDTPGP"
BASE COUNT          952 a         1857 c         1727 g          840 t
ORIGIN      
        1 tgccggagac atcagtgccg atccagccgg tgtcgacgtt gctcgcggtc agcgcccggc
       61 cgtcgacgat gacggccgga aacgcagcgc cgaccaatcc cttatagccg aagcccttca
      121 cgacctcgcc agcggtcttc gcgacatcac gcaccttcgc gccgggcggc gtgacgatgc
      181 gcacccgctc ggcggtcagc tcgccacgag cgaggtcaac cggggcgccc ttgataccag
      241 aaccgccaat gtcgagaccg aacacgcggg tactcagcct tccgagctgt cgtcggaacc
      301 gtcatttggg tctttcccgc cggccatctt gcgaacctga tcagcgaccc atccccccag
      361 gtttactgtt cccccgccga cgtcgcgcaa aaacttcatc agcggatcag gcgatcgatc
      421 aaacgactgc ttgtacgacg ccgctgcggc tttcacctcg tcgacgaacg acgcacgtgg
      481 atcgtcggca cgattgggat acgtgcctgc gaggatccgc tcgtactccc ccgacgcgac
      541 ccagcgcttg atctccagcg cgcgcagcac cgcgaacgga tgcgtcgagt tgaggatgtt
      601 cagaatccgg atcacgcctt cgcgcgcgtc accggtcgac tcatactctt cggcctgcgc
      661 gagaaacgcc tgcagatcca tgtcttcgac ccgcgcaccg cccgccatct tcatgttgac
      721 gcgggtgacc gctccggggt cttggctgac gagcagaccg gcgcggtcac acgacagttc
      781 ggccttgcgg taccactcct tcagcgcggt gatcacggcc cgcaggccaa ggccgccgag
      841 cgggatgatc gtgctcactc cggtgatgcg catcaacgca aacagcaccg acgagtagag
      901 cgcgtggccc gagagcacat gcccgagttc gtgacccaac accacccgca actcgtcgtc
      961 atcgagcaga tcgaccgtgc cggtactgac gacgatgaac gggttgtcga gacccaacgc
     1021 ccacgcgttg acccgcggat cctggttgat gtacagctcg ggcaacttct cgatgtcgag
     1081 aatccgggcg gcgtcctggt agatcgcgta aatacccgag aactgccgct cgtcgactcg
     1141 caccgcagat gcgagataca tcatgcggag gcgacgttca ctgaagaacc cgaacatgcc
     1201 tttgagcagg acgtcgaaac ccggcaccga ccgcaacgtg acaagcgcgg cccgatcggc
     1261 cggatgttcg taagcccgcg agctgatccc tggaaatgcg acgcgacccg tcggtgcctt
     1321 cgtcttgcca atggtagtcg tcttgcgcgg acgccgcgtg ccccccgaac cctgcgcatc
     1381 agccatgtcg tccacgctac gcctgcgcca aaccgcgggc ggccgcggtc gccgaccacc
     1441 gctcgagggt cgccgctctc gctggcgcgg tctgcgcttg caccacgagg aacagcgcct
     1501 cgcgagcctt gcgctgcgca ggcgacccga cattcatcgc tggtccggag ttcgcagcga
     1561 ccagcgccga cgtgactccg atgagcagtt cgagggcgat ggctcgaagc tcaaggcgct
     1621 cgtcgatggc gtcgcccggc ggaacagtat cggctagtgc ataacaacgc gtgcggacgt
     1681 cggccaggcg cgcgccgaag atctccgccg tgcgtggcgg ctggctcgtc tcgagcgacg
     1741 cctgcggtgc gcaggagctc gacggcactc gccgcgacgc cgaagaccgc cgcgttcgcg
     1801 ttcgcgacca gggtgtcgcc cgcgagccac tgtgcgtgcg actcgcgcaa ggcgacgtca
     1861 tggtcggcga cgaagacgcc gtcgagtccg agcacggccg tgcacgcggc gtcgagcgcg
     1921 gctgtccgca acgccgacct cacgctcagg ccgccgccct cgcgcgccgg caccgcaccg
     1981 aagatcacct caccctcgtc ggtaacgccc gaaaggaacg cgacgtcgtt caggccccac
     2041 ccggtgtacc aaggcgctac tccgtcgagg cgccacccgc catcgacggg ccgcgcgacc
     2101 actggccggt cgggaaaccg gcgcaaatgc gagaacgcga tgccggcgat cagctccccc
     2161 gatgcgagcc gagccaggta ctgcggcgcg gcggggctcg gggtgctgac gagcatccga
     2221 acgaccgagt gatgttgtgc ctgcacgaac caggtagcgg cgtcggcgcc cgcgaggagc
     2281 tcgttcacct cacggaagac ggccgcagag gcgtcggcgc cgcccacctc agcgggtgcc
     2341 agcaagccga gcaacccggc gtccgccaaa gcttgcagat gtgaacgagg gacgaccgat
     2401 gcgtcgacga tggcggcctg gggctcaagc agctctgcgg ccaaccggcg ggcggcctcg
     2461 acgaggggat gcccagccac ttccgtcacg ccagtcaacc tagcaatcgg ccgcagcctt
     2521 tctctgcaaa agctcacgtg cgggtcggtg cctgacggcc ggacacgcga ccgagagaat
     2581 gaccggcatg gcacgcaaat ccgcagcaac gccgccccct ccgcccggtg tcgagcagat
     2641 cgtcgacatc gacgtcagtg aggagatgcg cggcagtttc ctcgaatacg cctattcggt
     2701 gatctacagc cgcgcgctcc cagacgcgcg cgacgggctg aagccggtgc atcggcgcat
     2761 cctgttccag atggcggcga tgggattgcg ccccgaccgg ccgcacgtga agtgcgcccg
     2821 ggttgtcggc gacgtgatgg gcaagctgca cccgcacggc gacaccgcga tctacgacgc
     2881 actcgttcgg atggcacagc cgttctcgat gcgggtgccg ctggtcgacg gtcacggcaa
     2941 cttcggctcg ctcggcggtg acgatccgcc cgctgcctac cgctacaccg aatgccgcct
     3001 cgagagtgcg gccgagttga tgaccgatgg cctaagcgaa gaggtcgtcg acttcgggcc
     3061 gaactacgac ggccaggaag aggagccagc ggtactgcct gcggccttcc cgaacctgct
     3121 cgtcaacggc gcgaccggaa tcgccgttgg catggcgacc agcatggcgc cgcacaacct
     3181 cgtcgaggtc atcgccgctg ctcggcacct gatcaagaag cccgacgcca cgctcgacga
     3241 cttgatgcgc ttcgtgccag gccccgacct gcccaccggc ggcaagatcg tcggcctcga
     3301 cggggtgcgc gacgcgtacg agaccggtcg gggcatgttc cgcatcagag ccaccgcaaa
     3361 ggtgaccgac gtgcgcccgc gcaagcgcgg catcgtggtg acggagctgc cttacggcat
     3421 cggccccgag aaggtcatcg ggaagatcaa ggatctcgta cagtcacgca agcttcaggg
     3481 catcgctgac gtgcgcgacc tcaccgatcg cgagcacggg ctgcaactcg tcatcgagat
     3541 caagaacgga ttcaacgcag aggcgatcct cgaccagctc tacaaactca cgccgatgga
     3601 agacacgttc ggcgtcaaca acgtcgcact cgtcgatggc caacctctca cgctggggtt
     3661 gaaggagctg ctcgaggtct atgtcgacca ccgtttcgac gtcgtgcgcc ggcgcacgac
     3721 ctatcgccgc cgtcgccgtg aagagcaact ccatctcgtg cagggccgcc tcatcgcgat
     3781 cgtcgacatc gacgaggtca tcgcggtcat tcgctcgagc gacgacaccg ccgcagcgcg
     3841 cacgcggttg atggaggtgt tcgacctcag cgagatccag gcgaacgaca tcctcgagtt
     3901 gccactgcgg cgcctgacca agttctctcg tctcgagctg gagaaggaac gcgatcagct
     3961 gagccgcgag atcaccgaac tcacggcgat cctcgagtcg gacgagctgc tccgaaccgt
     4021 cgtgtccgac gagctcggcg acgttgcaaa gaaattcggg acgccgcggc gtaccgtcct
     4081 gctcgagtcg gccggcgtcc ccagcgccgc agtgcccctc gaggtgaccg acgacccgtg
     4141 tgtcgtactg ctctcagcga ccggactgct cgcgcgtgcg cagccgaccg acgacgctcc
     4201 cgtcaccgag gggcgaacac ggcacgacgt catcgcatcg aaggtgccat cgacggcgcg
     4261 cggcgaagtc ggcgtggtca cctcattcgg ccggatgatt cggctgtcag tgctcgagtt
     4321 acccgcgctg ccgccgaccg tcggcgcccc gacgttggcc ggtggtgctc cggtgcacga
     4381 gttcctcgaa ctggagaaga ccgagaacgt ccttgcgctg accaccttgc tcccgacatc
     4441 gcacggcgtt gccctcggta cggcgtccgg tgtggtgaag cgggtgctgc ccgactaccc
     4501 ctcgaacaag gactcgttcg agctgatcgg cctgaaggac ggcgacaccg tcgttggcgc
     4561 ggtcgaactc acctcgggtg acgaagagct cgtcttcatc acttccgacg ctcagctgtt
     4621 gcgctttgag gcggcgagtg tccgcccgca gggtcgatcg gcgggcggca tggccggcgt
     4681 ccggctgtcg agtggtgcgc acgtcgtctt cttcggagcg gtgccggtcg aaaccgatgc
     4741 cgtcgtcgtc accatcgctg gctcgtcggc ggcgctgcca ggtacagatg caggagcggc
     4801 gaaggtcgcg ccctactccg agtacccgcc gaagggccga gcgaccggcg gcgttcgatg
     4861 ccaccgattc ctgcggggcg aagacaccct tctggtcggc tgggcaggcg ccatacccgc
     4921 gaaggccacg gcgagctcag gtgtcgcgat cgagttgccc gcgccgaacg gccgacgcga
     4981 cggttccggc acaccgctgg cgtcccccgt gctggctatc gggaactgac cttcccccta
     5041 ggggattccg gcctagaatc cgattagcgc acccgcgcga gggaattgat cggtgtaagg
     5101 aggtgccgtg tccgaaggtg caccacagct gcacgtgtcg tctgagccga cctgggttgt
     5161 cgtcggtctc gacaacggtg gcacctgcaa caacgccaca gtgctgaccg attcggggga
     5221 gtttctcgtc gaccacctgg tcgagaaccc gagtcgcgtg aacgacggac ctgagatcgc
     5281 cgtcgccagt ttggcggaca gcctcgaggc gatcatcgac ctcaccggcg tgccgcgcat
     5341 gcacgtgcgc gcggtcggcc tcgatacgcc tggccc
//
LOCUS       DASWVF010000392         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_265299, whole genome shotgun sequence.
ACCESSION   DASWVF010000392 DASWVF010000000
VERSION     DASWVF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265299"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1686)
                     /gene="katG"
                     /locus_tag="VGD55_07095"
     CDS             complement(<1..>1686)
                     /gene="katG"
                     /locus_tag="VGD55_07095"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY0870147.1"
                     /translation="DNGNLDKARRLLWPVKQKYGRALSWADLMVFAGNCALESMGFKT
                     FGFAGGRADVWEPDEDVYWGPEETWLGDDRYTGERELESPLAAVQMGLIYVNPEGPNA
                     HPDPLASARDIRETFSRMAMNDEETVALIAGGHTFGKTHGAADPAQFVGPEPEGAPLE
                     EQGLGWKQSYGSGKGRDAITSGLEVTWTPTPTTWDNGFFEILFGYEWEQTLSPAGAHQ
                     WQPKDGGGANTVPDPEDGSLVRPPTMLTTDLALRFDPVYEGISRRFLENPDQFADAFA
                     RAWFKLTHRDMGPIQRYLGPLVPQETLIWQDPIPAVTHELVGAQAIAALKDQLLASGL
                     SVSQLISTAWASASTFRSSDKRGGANGARVRLEPQIGWDVNNPSELSTVLRVLEGIQQ
                     GFNDGFNAAGKHVSLADLIVLGGCAAVEQAAKNAGHDITVPFTPGRADASQEQTDVES
                     FAALEPTADGFRNYFGKGHRLPAEYLLVDRANLLNLSAPEMTVLVGGLRVLGANHDKS
                     KLGVLTTAPESLTNDFFVNLLDLGTTWKQTSADASTFEGRDSSGAVKWTGSRV"
BASE COUNT          291 a          561 c          530 g          304 t
ORIGIN      
        1 caacgcgact gcccgtccac ttcacggcac cgctcgaatc gcgtccttcg aacgtcgatg
       61 cgtcggccga agtctgcttc caggtggtgc ccagatcgag cagattcacg aagaagtcgt
      121 tggtgagcga ctcaggcgcc gtcgttagca cccccaactt cgacttgtcg tggttcgcac
      181 ccagcacccg caggccgccg acgagaactg tcatctcagg tgcactcagg ttcagcaggt
      241 tcgcgcggtc gacgaggagg tattcggccg gcagccggtg gcccttgccg aagtagttcc
      301 ggaatccatc ggcggtcggc tcgagcgcag cgaaggattc gacgtccgtc tgctcctgcg
      361 atgcgtctgc gcggcccgga gtgaagggaa ccgtgatgtc gtggccagca ttcttggcag
      421 cctgctcgac ggcggcacac ccgcccagca cgatcaggtc ggcgagcgaa acgtgcttcc
      481 cggcagcgtt gaagccgtcg ttgaaaccct gctggattcc ttcgagcacg cgcagcacag
      541 tcgacagttc actgggattg ttgacatccc aaccaatctg cggctcaagg cggacgcggg
      601 ctccgttcgc gccaccacgt ttgtcgctgc tgcgaaacgt cgaggccgac gcccaggcgg
      661 tcgagatgag ttgtgagacc gacagccccg acgcgagaag ctgatccttc agcgccgcaa
      721 tagcttgggc tccaacgagc tcgtgcgtga cggccggaat cgggtcttgc caaatcagcg
      781 tctcttgcgg cactaacggg ccgaggtagc gctgaatcgg acccatgtcg cggtgggtca
      841 gcttgaacca ggcccgcgca aatgcgtccg caaactggtc gggattttcg aggaagcgcc
      901 gtgaaattcc ttcatacacc gggtcgaatc gaagcgccag gtcggtcgtg agcatcgtcg
      961 gcggtcgcac gagcgagcca tcctctgggt cgggcaccgt gttcgcaccg ccgccgtcct
     1021 tcggctgcca ctggtgggcg ccggcggggc tcaaggtctg ctcccactcg tagccgaaca
     1081 ggatttcgaa gaagccgttg tcccaggtcg tcggcgttgg cgtccaggtg acttcgagcc
     1141 cacttgtgat cgcgtcgcgc cccttgccac tgccgtagct ctgcttccaa ccaagaccct
     1201 gctcttcgag cggagcgccc tcaggctccg gcccgacgaa ctgcgcgggg tcagcagcgc
     1261 cgtgcgtctt gccgaacgtg tggccgccgg cgatcagtgc gacagtctcc tcgtcgttca
     1321 tggccatccg gctaaacgtc tcgcggatgt cgcgagccga ggccaacggg tcggggtgcg
     1381 cgttcgggcc ttctgggttc acatagatca agcccatctg gacggcggcg agcggggact
     1441 ccagctcacg ctcaccggtg taacgatcgt caccgagcca ggtctcctcc gggccccagt
     1501 agacgtcctc gtcgggctcc caaacgtccg cacgaccacc ggcgaaaccg aaagtcttga
     1561 agcccatcga ctccaacgcg cagttgccgg cgaacaccat caggtcggcc cacgagagcg
     1621 cgcgcccgta cttctgcttc accggccaca gcagccggcg cgccttgtcg aggttgccat
     1681 tgtcgg
//
LOCUS       DASWVF010000393         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_315859, whole genome shotgun sequence.
ACCESSION   DASWVF010000393 DASWVF010000000
VERSION     DASWVF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315859"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1593
                     /locus_tag="VGD55_07100"
     CDS             76..1593
                     /locus_tag="VGD55_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0870148.1"
                     /translation="MRVASEHPAGVLEIATPARKSTLSRAVKAGGAIRLAPSIFVLGA
                     TLPAEQAVNHHRFAIAAQIWPGAVICDASALAGGEATAGWMFICHPDPPRSSDLHLPG
                     LSIAPRVGPGRLPGDMALPFGLSLSGPARTLVENVPLGGPQPRSRPPRAAGVEAVEDR
                     IDAEARTGGAGRIRAQLTQLDVIASSLPTKQVDLVRRRLAAVLGSFVGDAPPRSPRLR
                     ARLSGQPYDQHRIDMFAALAALLGDTAPQPRPALGGDERWRWLAFFEAYFSNFIEGTE
                     FGVEEARLIAIDGFEPADRPQDAHDVAATFRIASDPRLSHAVAKSGDELLDMLRAQHE
                     VLMAARPEKRPGQFKGKQNYAGGYVFVAPDLVRGTLLRGFDCFTGVTDPFQRAVALML
                     LLTECHPFDDGNGRIARLVSNATLSAAGQVRVVIPTVYRNNYLAGLSGVSNGAGRGET
                     LVSVLAFAQKWTASVDWSGWDFADVQMRDSNAYVDPGLAEQSGQRLRLASSLTGA"
     gene            complement(1579..>1961)
                     /locus_tag="VGD55_07105"
     CDS             complement(1579..>1961)
                     /locus_tag="VGD55_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870149.1"
                     /translation="LHGTQIAGLAVALNVPASVCGVACRQMAPGTSLLASLSAAEPAA
                     TGVPWTSMWTTDDQVVLPPDSARFAGATNLVLQQICADDAANHLNLPADPLVIGLTLQ
                     ALDTAKPSSPSASDCTGLRALGAS"
BASE COUNT          322 a          615 c          688 g          336 t
ORIGIN      
        1 ccataagagt tgcatgctgg cctcggttag gctgaaatag ttgcaggatg atgagattgg
       61 agggccggtt agcttatgcg cgttgcatca gagcatccgg cgggcgtcct tgagattgcc
      121 actccggccc gaaagtcgac cctgtctcgt gcggtcaagg cgggcggcgc gatccgactc
      181 gctccgagca tcttcgtcct aggcgcaacc ctgccagccg agcaggccgt caaccaccat
      241 cggttcgcga tcgccgcgca gatctggcca ggcgcggtta tttgtgatgc gagcgcactc
      301 gccggcgggg aggccaccgc agggtggatg ttcatttgcc atccggatcc gccccggagt
      361 tccgacctgc accttccggg tctgagtatc gctccacgcg tcggaccggg tcgactgccg
      421 ggagatatgg ccctgccctt cgggctgtcg ttgtcggggc ctgcgcggac gttagtggaa
      481 aacgtcccgc tcggcggccc gcagccgaga tcgcgtccac ctcgtgcggc aggagtcgag
      541 gcggtcgagg acaggattga cgccgaagcg cgcacgggcg gggccgggcg gatccgcgcc
      601 cagctcacgc aactcgatgt gatcgcttcc tcgttgccca ccaaacaggt ggatctggtt
      661 cggcgtcgcc tcgcggcggt gctgggaagt ttcgtcggcg atgcgccgcc gcgctcgcct
      721 cgactacgcg cccgcctgtc aggtcagccg tacgaccagc atcggatcga catgttcgcg
      781 gcgctcgctg cgctgctcgg cgacaccgcc ccgcagccgc ggcctgccct gggtggcgac
      841 gagcgttggc ggtggttggc gttcttcgag gcgtactttt cgaacttcat agaaggcacc
      901 gagttcgggg tggaggaggc acgcctcatc gctatcgacg gcttcgagcc ggcggaccgc
      961 cctcaggatg cccacgatgt tgccgcgacg ttccgcatcg ccagcgaccc tcgcctgagc
     1021 cacgcggtgg cgaagtcggg cgacgagttg cttgacatgt tgcgcgcgca gcacgaagtg
     1081 ctcatggcag cccgaccgga gaaaaggccg gggcagttca agggaaagca gaattatgcg
     1141 ggcggctatg tgttcgtcgc accggacctg gtgagaggca cgctgctgcg cgggttcgac
     1201 tgcttcaccg gtgtgaccga tccgttccag cgcgcagtgg cgttgatgct cctattgaca
     1261 gagtgtcatc cgttcgacga cggcaacgga aggatcgcgc ggctggtctc caacgccacg
     1321 ctgagcgcag ccggccaggt ccgggtcgtg ataccgacgg tgtaccgcaa caactacctg
     1381 gcaggactgt ccggtgtgag caacggggca ggccgcggcg agaccctcgt gtccgtgctc
     1441 gcgttcgcgc aaaagtggac agcgagcgtc gactggagcg gttgggactt tgctgatgtg
     1501 cagatgaggg actcaaacgc ttacgtggat ccgggactgg ccgagcaaag cgggcaacgg
     1561 ctccgactag cgagttcgct aactggcgcc tagggcgcgc aagcccgtgc agtcggacgc
     1621 cgaggggctg gacggtttcg ctgtgtcgag agcttgcagg gtcaggccga tgacgagcgg
     1681 atcggccggc aagttgagat ggttcgccgc gtcgtccgca cagatttgtt gaagcacgag
     1741 gttcgtcgcc cccgcgaagc gcgcagaatc ggggggcagc acgacctggt cgtcggtcgt
     1801 ccacatcgag gtccacggca ccccggtggc cgcgggctcg gccgcactga gcgaggcaag
     1861 caacgaggtg cccggcgcca tctgccgaca agccaccccg cacaccgacg cgggcacgtt
     1921 gagcgcgacc gcgagcccgg cgatctgcgt gccatgtagg g
//
LOCUS       DASWVF010000394         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163011, whole genome shotgun sequence.
ACCESSION   DASWVF010000394 DASWVF010000000
VERSION     DASWVF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163011"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGD55_07110"
     CDS             <1..804
                     /locus_tag="VGD55_07110"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEY0870150.1"
                     /translation="PSVDVRYLELVRATYEAFWHLDVTVDFVRPDADLSAYKVVAIPS
                     LYLVSDDAAANLSRYVEAGGQLVCNFFSGIVDPTDAVRLGGYPGAFRQLLGVRVEEFY
                     PLLEHQTVTLADGGTGRVWTELLTPAGAQVVTTYADGPLRGSPAITRREVGTGVAHYV
                     TTFLDAPSTKRLLHGICDDAGVATRSTKNDVGAHSMSVDSIEAAGIETIRRRAEGASY
                     LFVINHTDREVDVPAAGVELLSGTPATGSVHVAAGGVAVVRETSTAGAG"
     gene            801..1625
                     /locus_tag="VGD55_07115"
     CDS             801..1625
                     /locus_tag="VGD55_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY0870151.1"
                     /translation="MSTVLAKQRQSMILEHVRRSGGVRVSELTELLGVSDMTVRRDLD
                     VLARHNLIEKVHGGATVASAPSMDEPGFVAKSSRELSEKEAIALAAARLVRPGTAIAL
                     SGGTTTFALAQRITHVRDLTIVTNSIRVIDVLQQAPGAPTVILTGGIRTPSDALVGPV
                     ADLAIRSLHFDIFFLGCHGMDPHAGLTTPNLAESETNRAFIRGARQVVLLADHTKWGT
                     VGLSSFADLDEADVLITDPGLSDDARTAASEHIDRVIVAGTGPEAHDTALATVRQI"
     gene            complement(1638..2564)
                     /locus_tag="VGD55_07120"
     CDS             complement(1638..2564)
                     /locus_tag="VGD55_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY0870152.1"
                     /translation="MVPTGTQYRIGRGDERATITEVGGAVREYGQGHHEGAREIFQSY
                     PERDVSWAFHGSVLLPWPNRVRDGRYMFDGVEQQLALTEPLRRNAIHGLVAWQQWSLL
                     EHGVAHVALNCRTYPSPGYPFQLDTVVRYELVEAGLTVTTTSLNAGDTACPYAIGFHP
                     YVSTGAGVALDDCTLQIPASRRLLLDERLNPVGSEAVEGTESDFRAPRSLRGRFLDDC
                     FTGVLPDSSGLSWVHLASPDGHVVDVWADSSFGFWQVYSGDALPEPLARRSLAVEPMT
                     AAPNAFQSGDGLLRLEPGDSVTTQWGAKLRQA"
BASE COUNT          460 a          939 c          883 g          419 t
ORIGIN      
        1 ccctcggtcg acgtccgcta cctggagttg gtccgcgcga cgtatgaggc gttctggcac
       61 ctcgacgtca ccgtcgactt cgtccgccct gacgccgact tgtcggcata caaggtcgtc
      121 gccattccca gcctgtacct cgtgagcgac gacgcagcag cgaatctgag ccggtacgtc
      181 gaagccggcg gccagctcgt ttgcaacttc ttcagcggga tcgtcgaccc caccgatgcg
      241 gtgcggcttg gtgggtaccc tggcgcgttc cgccaactcc tcggcgtccg ggttgaagag
      301 ttttacccgc tgctggagca ccagaccgtc actctcgcgg acggcggaac cggccgggtc
      361 tggaccgagt tgctgacgcc cgctggcgcg caggtcgtca cgacgtacgc cgacgggccg
      421 ctacgaggct cgcccgcgat cacccgccgc gaggtcggaa cgggcgtcgc ccactacgtc
      481 acgacgtttc tcgacgcccc ctcgacaaag cgattgctgc acggaatttg cgacgacgcg
      541 ggtgtcgcga cgcggagcac caaaaatgat gtcggggctc atagcatgag cgtcgacagc
      601 atcgaggcgg ccgggatcga gacgatccgc cggcgagccg agggcgcaag ctacctgttc
      661 gtcatcaatc acacggatcg ggaggtcgat gtgcccgccg ccggagtcga attgctgagc
      721 gggacgcccg cgaccggttc ggtgcatgtc gccgctggcg gcgtagccgt cgtgcgagaa
      781 acctctaccg ccggggccgg gtgagtacgg tgctcgcgaa gcaacggcaa tcgatgatcc
      841 tcgaacacgt caggcgctct ggcggggtgc gggtcagcga actcaccgaa ctgctcggcg
      901 tctcagatat gacggttcga cgcgaccttg acgtgttggc gcgacacaac ctcatcgaga
      961 aggtgcacgg cggcgcgacg gtcgcgtcgg ccccgagcat ggacgagccc gggttcgtcg
     1021 cgaagtcatc acgtgagctt tcggagaagg aagccatcgc gctggcggcg gctcggctcg
     1081 tgcgccctgg cacggcgatt gcgctctccg ggggcacgac caccttcgcc cttgcgcaac
     1141 gcatcactca tgttcgtgac ctcaccatcg tgaccaactc gatccgggtc atcgacgtcc
     1201 tccagcaggc cccgggcgcg ccgacggtga ttctcactgg cgggatccgc acgccgtccg
     1261 acgcgctggt gggtccggtc gccgatctcg cgattcgatc gctgcacttc gacatcttct
     1321 tcctggggtg ccacggcatg gatccgcacg ccggcctcac caccccgaac ctcgcggagt
     1381 cagagaccaa ccgggcgttc atcaggggcg cgcgtcaggt cgtactgctt gccgaccaca
     1441 cgaagtgggg cacggtgggg ctgagctcgt tcgctgacct cgacgaggcg gacgtgctga
     1501 tcaccgaccc cggcttgtcc gacgacgccc gcaccgcggc gtccgagcac atcgaccgcg
     1561 ttatcgtcgc cggcacgggg cctgaagcac acgacaccgc gctcgcgacc gtccgccaga
     1621 tctgagccgc gcgctcgtca cgcctggcgc aacttcgcgc cccactgcgt cgtgaccgag
     1681 tcgcccggct cgagtcgcag cagcccgtcg ccgctctgaa aggcgttggg cgccgcggtc
     1741 atcggttcga cggcgaggct gcggcgcgcc aacggctcag gcagcgcatc accggaatag
     1801 acctgccaga agccgaaact cgagtcggcc cagacatcga ccacgtgacc gtcggggctc
     1861 gccaggtgca cccacgaaag gccagaacta tcaggcagga cgccggtaaa gcagtcgtcg
     1921 aggaagcgtc cgcgcaacga tcggggcgcc cggaagtcgc tctcggtgcc ttcgaccgcc
     1981 tccgagccga cggggttcag ccgttcgtcg agcaagagcc gccgcgacgc cgggatctgg
     2041 agcgtgcagt cgtcgagagc aacgcccgcg cccgtcgaca cgtaggggtg gaaaccgatc
     2101 gcgtacgggc acgcggtgtc gcccgcgttc agcgacgtcg tcgtcacggt caggcccgcc
     2161 tcaaccagtt cgtagcgcac cacagtgtcg agttggaacg ggtaaccggg gcttgggtag
     2221 gtccggcagt tcaaggcgac atgggcgaca ccatgctcga gcagcgacca ctgctgccac
     2281 gcgacgaggc cgtgaatggc gttgcggcgc aacggctcgg tgagagcaag ttgctgctcg
     2341 acgccgtcga acatgtaccg cccgtcgcgc acccggttcg gccacggcag caagaccgag
     2401 ccgtggaacg cccacgaaac gtcgcgctcg ggataggact ggaagatctc gcgtgcaccc
     2461 tcgtgatgcc cctgcccgta ctcacggacc gccccgccga cctccgtgat cgtcgcgcgc
     2521 tcgtcgccgc gaccgatccg gtactgcgtt ccggtcggca ccatcccgcg agactagtga
     2581 cccgcgccgc ggcgcacaga atccgacatg ccgcagcccc gtggatatcc tccctaggca
     2641 cgcggcccgg cggcggaccc gcgctaccat agccaaacga gaccgaacaa tgttcattat
     2701 c
//
LOCUS       DASWVF010000395         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_392706, whole genome shotgun sequence.
ACCESSION   DASWVF010000395 DASWVF010000000
VERSION     DASWVF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392706"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VGD55_07125"
     CDS             <1..964
                     /locus_tag="VGD55_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEY0870153.1"
                     /translation="MVTKTPKAPIELAGGVTVRTFTPPPDGFDPIKADDRHLLIHGYP
                     RRPDDPDLRERWESVLSRPFHLIEPTFREMPERRRRLPVRTESVATVAQTHGVENTDI
                     WSGAVVHAPKGDRFTWVEGTWTVPNAFPPVHASDGVWYSASTWVGIDGIDGSGDVLQA
                     GCDSDVMTFWGSTSRQLRPWWEWFPAGSYWISNLPVAQGDTLNCLICVTANSTTSAEI
                     FMYNLTSGIAASFAATAPSKTSLLGNSAEWVVERLEIDTSTPELARYGEVYFDECNAG
                     TLNRQLIKGGTGNTINMTDGGSVISTGTIETPSLIQVKYTGPNS"
     gene            complement(982..1917)
                     /gene="truB"
                     /locus_tag="VGD55_07130"
     CDS             complement(982..1917)
                     /gene="truB"
                     /locus_tag="VGD55_07130"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0870154.1"
                     /translation="MPGSAPEPRRRGGAGDPPLRGGLVVVDKPAGLTSHDVVARVRRL
                     AGTKRVGHAGTLDPMATGVLLIGIGRATRLLGHLALHDKDYVATMRLGQTTSSDDAEG
                     EVLTSTPAAHLAPDVIAQAVAGFVGEIKQRPPAVSAIKVDGKRAYARARAGEDVVLAE
                     RPVTVSRLDVIAVRPVEGEDTALLDVDVEVTCSTGTYIRALARDVGATLGVGGHLSAL
                     RRTRVGAFDLTSARGLAELETDFTMTPLDEVAAATFARRDVAADEAAALSHGGTLPAS
                     GMGDVVVAAFGPGDVFIALLEDDEDRARTRAVFVD"
BASE COUNT          356 a          696 c          646 g          322 t
ORIGIN      
        1 aatggtaacg aagacaccta aggcaccgat cgagctggca ggcggagtca ccgtccgcac
       61 cttcacgccg ccgccggacg gcttcgatcc gatcaaggcc gacgaccggc acctactgat
      121 ccatggctac ccgcgacggc ccgacgatcc cgacctgcgc gagcgctggg agagcgtgct
      181 cagccgtccg ttccacctga tcgagcccac cttccgagaa atgccagaac ggcgtcgccg
      241 attgccggtc cgcaccgaat cggtggcgac ggtcgcgcag acccacggcg tcgagaacac
      301 cgacatctgg tcgggtgcgg tcgtacatgc acccaagggt gaccgcttca cgtgggtcga
      361 gggcacctgg accgtgccga acgcattccc gcccgtgcat gcaagcgacg gcgtctggta
      421 ctcggcgtcg acgtgggtgg ggatcgatgg catcgacggt tcgggagacg tgctccaggc
      481 cggctgcgac tccgacgtca tgacgttttg gggttcgacc tcacgtcagc tgcggccgtg
      541 gtgggagtgg ttcccggccg gctcgtactg gatcagcaac ctgccagttg cgcagggcga
      601 cacgctgaac tgcctgatct gcgtgacagc gaactccacg acgtccgctg aaatcttcat
      661 gtacaacctc accagtggca tcgccgcctc gttcgctgcc accgcaccca gcaagacctc
      721 actgctcgga aactccgcgg aatgggtcgt cgagcgactc gagatcgaca ccagcacgcc
      781 tgaactcgcg cgctacggcg aggtctattt cgacgagtgc aacgcgggca ccctgaaccg
      841 ccaactcatc aagggcggca ccggcaacac gatcaacatg acggacggcg ggagcgtgat
      901 ctcgacaggg acgatcgaga ccccaagcct gatccaggtg aaatacacag ggccgaactc
      961 gtagcgcttc gagctaaccg ttcaatcgac gaagaccgcg cgcgtgcgtg cgcggtcttc
     1021 gtcgtcttcg agcagcgcga tgaaaacgtc gcctggtccg aaggcagcca cgactacatc
     1081 acccatgccg gacgccggga gggtgccgcc gtgcgacaac gccgccgcct cgtcggcagc
     1141 gacgtcacgt cgtgcgaacg tggctgctgc gacctcgtcg agcggcgtca tcgtgaagtc
     1201 cgtctcgagt tcggcgagcc ctcgcgcact cgtaaggtcg aacgcgccca cccgggtgcg
     1261 acgcagcgca ctcaagtgtc caccgactcc aagcgtcgcg ccgacatcgc gtgcgagtgc
     1321 tcgaatgtag gtgcccgtcg aacaggtcac ttcaacgtcg acgtcgagca aagcagtgtc
     1381 ttctccttcg acgggccgta cggcgatcac gtcgaggcga ctcacggtca ccgggcgctc
     1441 ggccagcacg acatcttcgc cggcacgggc gcgggcgtag gcgcgcttgc cgtcgacctt
     1501 gatcgcactt acggcgggtg gccgctgctt gatctcaccg acgaatcccg cgaccgcctg
     1561 cgcgatcaca tcgggtgcga gatgggccgc gggtgtcgac gtcagaacct cgccttcggc
     1621 gtcgtctgac gacgttgtct ggcctagccg catggtcgcg acatagtcct tgtcgtgcag
     1681 cgccaagtgg ccgagcagtc gggtcgcgcg gccgattccg atgagcagta caccggttgc
     1741 catcgggtcg agagtgccgg cgtgcccgac acgtttcgta ccagcgagtc gtcgcacccg
     1801 ggccacgacg tcgtgcgatg tcagaccagc gggcttgtcg acaacaacga ggccgccgcg
     1861 cagcggcggg tcgccagcac caccgcgtcg ccgcggctcg ggtgcactcc ccggcactta
     1921 ctccgccgca cctggcgcaa cgcggtacgg gtcggcgtcc cctgccggaa ccgcgtgcgt
     1981 cgcgacctcg tgcacggccg cgtcagcctc gcgcgctttc
//
LOCUS       DASWVF010000396         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_214438, whole genome shotgun sequence.
ACCESSION   DASWVF010000396 DASWVF010000000
VERSION     DASWVF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214438"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VGD55_07135"
     CDS             complement(<1..175)
                     /locus_tag="VGD55_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870155.1"
                     /translation="MNETLATPPSAPLLRCDNLVHVYESSGSRVAALRGIDLVVESGE
                     TVALLGPSGSGKST"
     gene            complement(175..3240)
                     /locus_tag="VGD55_07140"
     CDS             complement(175..3240)
                     /locus_tag="VGD55_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870156.1"
                     /translation="MRLPVVVRGLGYRRGSTAAMLTIAAVGCAAAALAPTYDAAAKTS
                     IMRDTVSSASVIERGFEVREQGSLAHVLDPLSATVDRIVASTAPSGDRPLFASKVEAV
                     EATAYFSEQDQSAPLIYRSDLCAHLTMLAGKCELSTGVVMVSESLAKLNGWQVLQRIT
                     PSGWSTFTISGIYHTPDPSAAYWFDQGNLYFPAEYPASIVDVPPIDAVFTSQDTIESA
                     TGYHSAIDTVDELINQSTLRTTDLPKLTATVTALSNSQDLIQADASAESSMQATVDQV
                     RSSWHSLSVPVVLITAQTLLLVWLLLFVIVTEAIDARGRDIALAKLRGYGRRRVLGVA
                     VSEPAVVLVLAWPLGLVLAWFVSHFLVADLLRAGTPTGLTALSWLIALLGTVGGVAAV
                     SVGGRRAVRRPIVEEWRRVGQETTRRGWIVDSVALTAAAAALLELRLGGSGTPNKSLV
                     LLVPGLLGIAVAVVGSRVLPLVCRALFEFTARGGSLAVFLAVRHVARRPTGLRAVTAM
                     AAAFTLTSFGLASYATGHANRALVAEVSNGAPAVMTVDAKAGSNLGTVVAGLDPSRTQ
                     AMVVDQYLNPTGSGRVLLGIDPQPFAKIASWRSSFAAKPLADVLRALDPSAPPPVIVD
                     GDALRVRLDVRSESQPLVLAADLTVADQLAPIYANLGTITQTGAQVSLAATVTDCPCQ
                     LLDLSVSTTVGPGRGAAVPVQPDIVVNGLDERKAGTWVPVAAGTDDAHRWRTTVGDHA
                     PLAVSATSQGLQWSAGFMSNQVALLQPIDRPDPIPVVIGSTGAAALSGTGQLTGLNGR
                     PLAVVGVASAVVLPGAPTGGVLIDRAYAESAAGGNLTAVQQQVWTTGAVANRIHTALV
                     AAGFHVGDITRAAAQTRVLQRQGPGLASILFLGDSVAAAILAAGVAIAGLAFATRRRR
                     YEYAALEAVGERRSRLFLGLFLEQSMVLLFGALIGIGAGLVAARVVSSSIPVFVTKPA
                     GVPLSYSPSLSALTPTLLGSVGVLLCVAALTSWRLLAGVRPDQLREAAS"
     gene            complement(3237..4442)
                     /gene="hutI"
                     /locus_tag="VGD55_07145"
     CDS             complement(3237..4442)
                     /gene="hutI"
                     /locus_tag="VGD55_07145"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY0870157.1"
                     /translation="MASIRFDNIATLVTNDPANDAGDGSPLGVIHDASMVIEGDQVSW
                     VGHQAHPPDADVRVDLDGQTVIPGFVDSHSHLVFGGDRVEEFAARMSATPYTAGGIAT
                     TVAATRSADSAALQANARRLVAEMAASGTTTVEIKGGYGLTTHDEVRSQSIAHELTSE
                     STFLGAHVVPPEFHNRRDDYVALVAGEMLDACASSARWIDVFCDRGAFDVDEARLILE
                     AGVAKGLVPRLHAHQLSRTGAVELGVALVAASVDHCNAMNADDLDVLAGSSTLATVLP
                     GADFSTRSRYADARRMIDAGIVVAIATDCNPGTSYTTSMPFCIAVAVRDMRLTPAEAL
                     WAATAAGARALRRDDIGVLAVGMSADFAVVAAPSYVHLVYRPGVALVSATWKSGSQIV
                     PVQRFATPS"
     gene            complement(4442..>4969)
                     /locus_tag="VGD55_07150"
     CDS             complement(4442..>4969)
                     /locus_tag="VGD55_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0870158.1"
                     /translation="TQLRSDDISLLAGTSTGVCFCPTTERDLADGIGPAGALAANGVA
                     LSLGSDGHSVIDFFEEARALELDERLATHVRGTFGGVRLLEAATVAGHHALGWADAGQ
                     LAVGMRADLVAVDLDSRRTAGGLVDAETVVFAASASDVTDVIVDGVAIVSERQHLRVN
                     DIGEALRDAIDAVTD"
BASE COUNT          968 a         1660 c         1579 g          762 t
ORIGIN      
        1 gcgtcgactt gcccgaccca gaagggccaa gcagcgccac ggtctcgccg ctttcgacga
       61 cgagatcaat cccccgcagc gcagcaaccc gcgagcccga cgactcgtaa acatgcacga
      121 ggttgtcgca acgcaacagc ggggcggacg gcggggtggc gagcgtttcg ttcatcagct
      181 tgccgcctct cgcaactggt cagggcgaac ccctgcgagc aaccgccaac ttgtcagcgc
      241 agctacgcag agcagcactc cgacggaacc aagaagcgtc ggcgtcagcg cggaaaggct
      301 aggcgaatac gacagcggca caccggccgg cttagtcacg aacacgggaa tcgacgatga
      361 cacgactcgg gccgcgacta ggcccgcgcc aattccgatc aacgccccga agagcaagac
      421 catcgactgc tcaaggaaaa ggccgaggaa taaccgtgat cggcgttcgc caacagcttc
      481 tagtgctgcg tactcgtagc gccggcggcg ggtcgcgaac gccaacccgg cgatcgctac
      541 accggcggca agaatcgcag cagcaacgga gtcgccgaga aacaagatgc tcgccaaacc
      601 ggggccctgc cgctgcaaga ctcgggtctg cgctgctgcc cgcgtgatgt caccaacgtg
      661 gaagcctgca gccacaagag cggtgtggat tcgattcgca actgcccccg tcgtccacac
      721 ttgttgttgc acagccgtga ggttgccacc tgccgcgctc tccgcgtacg cccggtcaat
      781 cagcacgcca ccggtcggcg cccccggaag caccactgcg gaggcgaccc ccacaacggc
      841 gagcggtctg ccgttcagac ccgtcagctg accggtcccc gacagcgccg ctgcacccgt
      901 actgccaatg acgacgggga tcggatcggg ccggtcgatg ggctgtagca gcgcaacctg
      961 gttcgacatg aatcccgccg accattgcaa cccctgcgac gtcgcagaaa ccgccagcgg
     1021 cgcgtggtcg ccaaccgtcg tgcgccaacg atgggcatcg tccgtgcccg cggcgaccgg
     1081 cacccacgtg cctgctttgc gttcatccaa tccgttgacg acgatgtcgg gctgcacggg
     1141 caccgcggca ccgcgacccg ggcccactgt ggtgctgacg gacagatcaa gcagctggca
     1201 cggacagtcc gtcacggtgg cggcgaggct aacctgcgcg ccggtttgag tgatggtgcc
     1261 gagattcgca tagatgggag cgagctggtc agccaccgtg agatcggcag ccaacacaag
     1321 cggctgcgat tcacttctca catcgagtcg cacccgcagc gcgtcaccgt cgacgatcac
     1381 cggcggtggc gcacttgggt cgagggctct gagaacgtca gcaagtggct tggccgcgaa
     1441 gctcgatcgc cagctggcga tcttggcaaa aggctgcgga tcgatgccga gcagcacccg
     1501 gccactgccg gtggggttca agtactggtc gaccaccatg gcctgcgtgc ggctcggatc
     1561 gagcccggcg acgaccgtgc cgaggttgga tcctgccttc gcatcgacgg tcatcacggc
     1621 gggggcgccg ttgctcacct cggcgaccag cgctcgattc gcatgacccg tcgcatacga
     1681 cgcaagcccg aagctggtca gggtaaaagc ggccgccatc gcggtgactg ctcgcaggcc
     1741 ggttgggcgt cgtgcaacat ggcggacggc gaggaacacg gccagcgatc ctccgcgcgc
     1801 agtgaactcg aataaagccc ggcataccaa aggaagaacg cgagagccca cgacggcaac
     1861 ggcaattccg agcaacccgg gcaccaacag cacgagggac ttgttcgggg ttccgctgcc
     1921 acccagccgc agctcgagca gggccgcagc agcagcggtg agcgcgactg agtcgacgat
     1981 ccaaccgcgg cgcgtcgttt cctggccgac ccgtctccac tcctccacga tcggccgtcg
     2041 caccgcgcgt cgccctccta cgctgacggc cgcaaccccg cccacggtgc cgagaagggc
     2101 gatcaaccaa ctgagtgcgg tgagacccgt cggcgtgcca gcgcgcaaga ggtcggcgac
     2161 caagaaatga ctcacgaacc acgcgagcac caggccgagg ggccacgcga gcacgagcac
     2221 gactgccggt tctgacacgg caaccccgag cacgcgacgc cggccgtatc cgcgcaactt
     2281 ggcaagcgcg atgtcacggc cgcgggcgtc gatggcctcg gtgacgatca cgaacaacag
     2341 cagccagacc agtagcaacg tttgtgcggt gatgagtacg accggcaccg acagcgagtg
     2401 ccagcttgat ctgacctgat cgaccgttgc ctgcatgctg ctttctgccg aggcatcagc
     2461 ctgaatcaag tcttgggagt tcgacaacgc agtcacggtt gccgtgagct ttgggaggtc
     2521 ggtggtgcgc agcgtcgact ggttgatgag ctcatcgacc gtgtcgatcg cgctgtgata
     2581 tccggtcgcc gactcgatgg tgtcctgcga cgtgaacacc gcatcgatgg gcgggacatc
     2641 cacaatcgac gccggatact ccgcgggaaa gtacaggttc ccttggtcga accagtacgc
     2701 ggccgacggg tcaggcgtgt gatagatccc gctgattgtg aaggtgctcc agccgctcgg
     2761 ggtgattcgc tgaagaacct gccaaccgtt caacttggct agagactcac tcaccatcac
     2821 gacgccagtg ctgagttcgc acttgccggc aagcattgtg aggtgcgcac acagatctga
     2881 tcgatagatc aagggggcgc tctgatcttg ctcactgaaa tacgctgtcg cctcgacggc
     2941 ttcgaccttc gaggcgaaca agggccggtc acccgaggga gctgtcgatg cgacgatccg
     3001 gtcaactgtc gccgacaggg ggtcgagaac gtgcgccaac gatccttgct cgcgaacctc
     3061 gaatccgcgt tcgatcactg acgccgaact gaccgtgtct ctcatgatcg aggtcttggc
     3121 tgctgcgtca taagtcgggg caagagcggc cgcggcgcag ccgacagctg cgatggtcag
     3181 catggcggcg gtcgaaccgc gacggtaacc aaggccccga acgacgacag gcagcctcat
     3241 gacggcgtcg cgaagcgctg caccggcacg atctgcgacc ccgacttcca ggtggcgctg
     3301 acaagcgcga ccccgggccg gtagaccagg tgcacatacg aaggcgcggc aacgaccgcg
     3361 aagtcggcgc tcatgccgac cgcaagcacg ccgatgtcgt cgcgtcgtaa cgcgcgcgct
     3421 cccgctgccg tcgccgccca cagcgcctca gcgggcgtca gacgcatgtc gcgcaccgcc
     3481 acggcgatgc agaacggcat gctcgtcgtg tacgacgtgc cggggttgca gtcagttgcg
     3541 atggcgacga cgattccggc gtcgatcatg cggcgggcgt ctgcgtaccg gctgcgcgtg
     3601 gagaagtccg cgcccggcaa cacggtcgcc aacgtcgaag acccggcgag cacatcgaga
     3661 tcgtcggcat tcatcgcatt gcagtggtcg accgacgccg cgacgagcgc gacgccgagc
     3721 tcgactgcgc cggtgcgcga gagttgatgc gcgtgcagcc gcggcaccaa acccttggcg
     3781 acgcccgctt cgagaatcag ccgcgcctcg tcgacgtcga acgcgccccg gtcgcaaaac
     3841 acgtcgatcc atcgcgcgct ggaagcacac gcatcgagca tctcccccgc gacaagtgcg
     3901 acgtagtcgt cacgacggtt gtgaaactca ggcgggacga cgtgtgctcc caggaaggtc
     3961 gactcagaag tcaactcatg agcgatcgat tggctgcgca cctcgtcgtg ggtcgtgagt
     4021 ccgtagccgc ccttgatctc aacggttgtc gtgcccgacg cggccatctc tgccaccaac
     4081 cgccgcgcat tcgcctgcag cgccgcgctg tcggccgaac gagttgccgc gacggtggtc
     4141 gcgataccgc ctgcggtgta aggagtagcg gacatacgtg cggcgaactc ctcgacacga
     4201 tcgccgccga agaccaggtg gctgtggcta tcgacgaacc ccggaatcac ggtctggccg
     4261 tcaagatcga cccgcacatc ggcgtccggc ggatgggctt gatgtcctac ccacgacacc
     4321 tgatcgccct cgatcaccat cgatgcgtcg tggatcacgc cgaggggcga tccgtcaccg
     4381 gcgtcgttgg cgggatcgtt ggtcacaagc gtcgcaatgt tgtcgaaccg aatgctcgcc
     4441 atcagtcggt caccgcgtcg atagcgtcac gcagcgcctc gccgatgtca ttaacccgta
     4501 ggtgctgtcg ttctgaaacg atcgcgacgc cgtcgacaat cacgtcggtg acgtcggacg
     4561 ctgaagccgc gaagaccacg gtttcggcat cgacgagacc accagccgtg cgtcgagagt
     4621 ccaagtcgac cgcgaccagg tcggccctca ttccgaccgc gagttgaccg gcgtcggccc
     4681 aaccgagcgc gtgatgtccg gccaccgtcg ctgcttcgag caatcgcacc ccgccaaagg
     4741 ttccgcgcac gtgagtcgcc agccgttcgt cgagttcgag ggcccgcgct tcctcgaaga
     4801 aatcgatgac ggagtgtcca tctgatccaa gagacagcgc gacaccattc gctgccaacg
     4861 cgccagccgg cccgatgccg tctgcgagat cgcgctcggt ggtcgggcag aaacacacgc
     4921 cggtcgaagt tccggcaagc agcgagatat cgtcgctgcg cagctgggt
//
LOCUS       DASWVF010000397         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_367089, whole genome shotgun sequence.
ACCESSION   DASWVF010000397 DASWVF010000000
VERSION     DASWVF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367089"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGD55_07155"
     CDS             complement(<1..364)
                     /locus_tag="VGD55_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870159.1"
                     /translation="MVDAVRGRALGVADACVAGVAGCVADLAGAAVRRHPALGDDARG
                     VDAQIAAAVLVGASGVIGAARAHVRHAIADLTGVAVDGRAAVAGRAAAVEAELLRAVI
                     RDADRIIETRDAGEPRGIA"
     gene            1151..>2038
                     /locus_tag="VGD55_07160"
     CDS             1151..>2038
                     /locus_tag="VGD55_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870160.1"
                     /translation="MNNSCTGCSPATGNTYYVDPINGNDAGATGSAIVGGLATPSCSF
                     KTVTHALSVASSIGATGTKIVIVGQPGQTISLSPSETLPLVVPASVAISTKGGPIRVN
                     LSSASDTAFGGFRLVGDQAVIAPDPSAPLTIDGNANTSGIGIAVSPGSGKTASISYVT
                     VQNTGGDGIAVANGTLNIFQGVTVTNAGSAAKHHDGMNLSGGTVNIGVMAGQATTSFS
                     NNTEHGIYVTGRAVLNVVGVPVTSPAPNGQGTVIANNNGFDGLEIFETPFAAGQSSII
                     GLVAWGNRKHGIQLFGGEKV"
BASE COUNT          355 a          707 c          637 g          339 t
ORIGIN      
        1 ctgcgatacc gcgaggctca cctgcgtcgc gtgtctcaat gatccgatct gcgtcgcgaa
       61 ttaccgcgcg cagcaactct gcctcaacgg cagctgcacg cccggcaact gccgcacggc
      121 cgtcgactgc aacgccggtc agatctgcga tcgcgtgtcg cacatgtgct cgagctgcgc
      181 caatgacgcc gcttgcacca acgagcacgg cagcggctat ttgtgcatcg acgcctcgtg
      241 catcgtcgcc cagtgccgga tgtcggcgga ctgcagcgcc ggccaagtct gcaacacaac
      301 cagctacacc tgcaacgcat gcgtccgcga ctcccagtgc gcgtccgcgt acggcatcaa
      361 ccacctgtgc atcatgggcg cgtgcctctc gggcacgtgc cacaccacgg ccgactgcgg
      421 cggtggcgag ctctgcgacg acactaccca ctcgtgcgtc gcctgcggca gcgattccgc
      481 ttgcctgagc tcttacggcc cagagcacct gtgcatcaag aacgcttgcg tcagcggcca
      541 gtgccgcacc tccggtgact gccccaacgg ctcgctgtgc gacagcccga cccacacgtg
      601 ccatcgctgc gtcagcgaca ccgcctgcca ggtcgaccgg gcctacggtg taaccacggt
      661 ttgcgtgtct ggggcgtgtc aggctgggga ttgccattcg tcgtcgagcg ggtgcccgac
      721 cggtcaggtc tgcggcgtct cgcacgccga tacgtgtggc gcttgcgcga ccgactcgca
      781 ctgcaccggt gatccgatct acggcgccgg ctacatctgc tatcagggtc tgtgccagcc
      841 gggtgattgc cacggcacca gcgcggactg ctccggggcc gaggccggcc agatctgcgg
      901 cgccgtggat accaacaaat gcggaccgtg ctccagcgac ccgcagtgcc agtcggatcc
      961 gttctatgga tcctcacgca tctgccagac cgcaggcggt tccggcagcg ggacctgtgt
     1021 cagcgcggcc tgctccacga gcggcgcctg cggtgccaac cgcgtcgacg tctgctgcag
     1081 ctacatctgc acgcccggca actgctgcct cgacgccgac tgcgccagcg tggggtcgaa
     1141 ttccaggtgt gtgaacaaca gctgcaccgg gtgctcgccc gccacaggca acacctacta
     1201 cgtcgatcca atcaacggga acgacgccgg cgctaccggt agcgctatcg tcggcgggct
     1261 cgccacaccc agctgcagct tcaagacagt tacgcacgcc ctgtcagtcg ccagcagcat
     1321 cggcgcgacc gggacgaaga tcgtgatcgt cggccagccg gggcagacca tttcactcag
     1381 tccctccgag acgctaccgc tcgtggttcc cgccagcgtt gcaatctcga cgaagggcgg
     1441 cccgatccgg gtcaacctct cgtcggcgag cgacacggcg tttggtgggt tccgtttggt
     1501 cggggatcag gcagtcattg cgccagatcc gtcggcgccg ctgacgatcg acggcaacgc
     1561 gaatacttcg gggattggga tcgccgtgtc tccggggtcg ggtaaaacgg cgtccatttc
     1621 gtacgtcacc gtccaaaaca cgggcggcga cggcatcgcc gtcgcgaacg gcacgctgaa
     1681 catcttccag ggcgtgaccg tcactaatgc cggcagcgcc gccaagcacc acgacgggat
     1741 gaacctgtcg gggggcaccg tgaacatcgg cgtgatggcc ggccaagcca cgactagctt
     1801 cagcaacaac actgagcacg gcatctacgt gaccggcagg gccgtcctga acgtggtcgg
     1861 tgtcccggtc acctcgccgg cgccgaatgg tcaggggacg gtcatcgcga acaataacgg
     1921 cttcgacggc ctcgagatct tcgagacgcc cttcgcggct ggccagagct caatcatcgg
     1981 cctggtggca tggggcaacc gtaagcacgg catccaactc ttcggcggtg agaaggtc
//
LOCUS       DASWVF010000398         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_188949, whole genome shotgun sequence.
ACCESSION   DASWVF010000398 DASWVF010000000
VERSION     DASWVF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188949"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..>1745
                     /locus_tag="VGD55_07165"
     CDS             386..>1745
                     /locus_tag="VGD55_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0870161.1"
                     /translation="MTSRTNPFRTVSRFTAVAAVAALAVTACSSTHKTGSPATSSAAS
                     TPATSTSATSAPATTAASSAASPATGGQLNGGKPVTLTWWHNASTQPGLGAFQKVADD
                     FHAANPNVTISVQPIQNETIKTKIQVALQGTPPDIFQQWGGGAEAIQAQSGKLADLTQ
                     LTSSWIGQLGKSAAGWTDNGKQLGVPYDYHVVGFWYRTDLFAKAGISSPPSTMADLLA
                     DITKLKAAGITPMTVGGKDKWPDAFYWENFVLKECPEATITSSIAAAKFTDPCFVKAG
                     ADLKTLLDANPFNTGFLATPAQQGAGSSAGLVANGKAAMELQGDWEIPTIIGLVPTAQ
                     QATVEGEMGFFPFPSITGGNGEPGAALGGGDGFSCSTKAPDACAAFLQYFTSAPVEEE
                     LVKATAISIPSNGGATSAITDPTLQKVLTYLQGVSYNQLYFDQALPTAVGNAVNDAVS
                     NFF"
BASE COUNT          333 a          576 c          545 g          291 t
ORIGIN      
        1 cgccacggaa gatccgccgg tcgcggcagt tcggtcgtcg cgagcccgta ggcgggcggc
       61 tcctgtgtca ccacatcatg tccacttcgt cgtagcctgc gtccgggtgg gctgatcgag
      121 atgggccacc atccgcgaat cggtgctcgg gatagtaccg aaaactttcg gaagtgtctc
      181 gggcaggatc cgtctattct cacgcgaaga taacggttcg ggaaggttcc cgggaatggg
      241 ttgaccgcgt tcatgaggag ccataacgta gcggcgccgt cggaactatc gtgatgttct
      301 cggtaacttt cggtacgacc ggtgacgggc gtactcacct ccgggctccc gtgtcgagta
      361 cgggacccac gctgaaggga cgctcatgac gagccgcacc aacccattcc gcaccgtgtc
      421 ccgcttcacg gcagtcgccg cagtcgcggc gctagccgtc accgcatgct cgagcaccca
      481 caagaccggg tcgcccgcaa ctagctctgc ggccagcacg ccggctacca gcacatccgc
      541 aaccagcgcg cccgctacca ccgctgcaag ctccgcggca tcgcccgcta ctggtggcca
      601 gctcaacggc ggcaagcccg tgacactgac gtggtggcac aacgcgagca cccagcccgg
      661 cttgggcgcg ttccagaagg tggccgacga tttccacgcg gccaacccga acgtgaccat
      721 cagcgttcag ccgatccaga acgagaccat caagacgaag atccaggtcg cgttgcaggg
      781 caccccgccg gacatcttcc agcagtgggg cggcggcgcc gaggccattc aggcccagtc
      841 gggcaagctc gccgacctca ctcagctcac gtcgagctgg atcggtcagc tcggcaagag
      901 tgccgcgggt tggaccgaca acggcaagca gctcggggtc ccgtacgact accacgtggt
      961 cggcttctgg tatcgcaccg acctgttcgc caaggccggc atcagctcac cgccgtcaac
     1021 gatggctgac cttctggccg acatcacgaa gctgaaggca gcaggcatca cgccgatgac
     1081 cgtcggcggc aaggacaagt ggccggatgc gttctactgg gagaacttcg tattgaagga
     1141 gtgcccagag gcaacgatca cgtcgagcat cgcggccgcc aagttcaccg acccgtgctt
     1201 cgtgaaggcc ggcgccgacc tgaagacgct gctcgacgcg aaccccttca acaccggctt
     1261 cctcgcaact cctgcacagc agggcgctgg gagctcagcg ggcctggtcg ccaacggcaa
     1321 ggcagcgatg gaactgcagg gtgactggga aatcccaacg atcatcggtt tggtcccgac
     1381 ggcccagcag gcaacagtcg aaggcgagat gggcttcttc ccgttcccgt caatcactgg
     1441 tggcaacggc gagcccggtg ctgcgcttgg tggcggcgac ggcttctcgt gctcgaccaa
     1501 ggcgcctgac gcgtgtgctg cgttcctgca gtacttcacg agcgccccgg tcgaagagga
     1561 gctcgtgaag gcgacggcca tcagtatccc gtcgaatggt ggcgctacgt cggcgatcac
     1621 cgatccgaca ttgcagaagg tgctcaccta cctgcagggt gtgtcgtaca accagctgta
     1681 cttcgaccaa gcccttccga cggcggttgg caacgccgtg aacgacgcgg tgtcgaactt
     1741 ttttg
//
LOCUS       DASWVF010000399         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_392794, whole genome shotgun sequence.
ACCESSION   DASWVF010000399 DASWVF010000000
VERSION     DASWVF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392794"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGD55_07170"
     CDS             complement(<1..405)
                     /locus_tag="VGD55_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870162.1"
                     /translation="MTTATRRLRRTGVAGFAAAVTAGAMLIGTATNAFAAELAVSTQV
                     GATAATTVNPGTTAAALGDFNIGLPNNFHIGDTITVQLAGAGASCGTTNGAIGYSAAP
                     TVAASGPFTAAFGTTAGSSTDTTPTFTDVLQSS"
     gene            complement(613..1755)
                     /locus_tag="VGD55_07175"
     CDS             complement(613..1755)
                     /locus_tag="VGD55_07175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870163.1"
                     /translation="MRVLHLTSSFPRDARDHVAPFLLDLAHAEQEAGMDVGVLAPHDK
                     GLATVDTVAGVEVHRFRYGLQSMEKLSYRGGLLGSSRRFSGIMLPAYLASFSRAAEKA
                     ARDFRPDVIHAHWWLPAGLCAVPVARRLDVPLIITVHGSDVHLLRYRALRKLASHVMH
                     RASLVATVSEDLQRQILEFDPRLHTAVLRLPVPSGPAAAPMPADPPLRLLAAGRLSHE
                     KGFDVLIEAMRIAVGSGLDVRLDIVGSGPERRRLALLAAPLGDRIQLIPALPHAELMH
                     RLEGVHALVAPSRREGLGMVALEAFTRGRPVVASRVGGLAESVEDGVDGYLVEPEDPI
                     ALAAALARLPFGSPRGVATLRHSSIEVGRSHLEAYASLVSPMVKSR"
     gene            complement(1846..2976)
                     /locus_tag="VGD55_07180"
     CDS             complement(1846..2976)
                     /locus_tag="VGD55_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0870164.1"
                     /translation="MAPRVLIDASAVPADRGGLGRYVDGLLAALAAKDADLAVVCQRV
                     DAERYARLVPAAQIVAGPASLTNRTARIAWEQTGLPLVAEHVAAKVIHAPNYSVPMRP
                     GRPVVTTIHDATFFSEPDLYPPTRGAFYRAAIRSAVKRSTRCIVPSKATRDELVRLVD
                     ADSTRIDVAYHGVDHEVFRPPTEAEAARISARLGLRGQQYIAFLGAFEPRKNVPSLIR
                     GWVSAVGQWDAPPALVIAGGAGWDDEIEEAVAEVPSHLRVIRPGYLRMADLSAFLGGA
                     LVVAYPSHGEGFGLPVLEAMACGAAVLTTHRLSLPEVGGDAVAYTEPDSASIGTALSG
                     LLADEPRRRALGSAAHARSLEFTWEASAQAHLVAYTRAVSGD"
     gene            3120..4112
                     /locus_tag="VGD55_07185"
     CDS             3120..4112
                     /locus_tag="VGD55_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0870165.1"
                     /translation="MVGSAATDRSVVATVVVVAWNGAELIGPCLDALVEQELADGFVT
                     WVIDNASTDSTGSVVRQHPVGARLVSGARNLGFAGAAELAVREATTPLVVVLNQDARP
                     EPGWLAALLAPLTGPDAHRIAAVTSKVIFTADGRLNNTGVIVGRDGYGRDRGFGQPDD
                     GRFASAEDVFAFSGTAAALRVVAARDVGSFDPSFFLYYEDTDLSWRLQLGGWRVRYAP
                     DAIVHHVHAASTDVSSAAFAFYNERNRLLMLAKCAPRTLAVKEVLRFAYITFALPLRR
                     VMRVDVPTGHQFRTATRVRALASFARMLPAVLQKRRAVSRICRRSRAEVARELS"
     gene            complement(4183..>4389)
                     /locus_tag="VGD55_07190"
     CDS             complement(4183..>4389)
                     /locus_tag="VGD55_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870166.1"
                     /translation="VDGSVALQQTASISRPDVAADYPSFGAAHGFAFPVGVKAGKHTV
                     CAFGINVGRGDTNTSLGCTSVNTS"
BASE COUNT          772 a         1435 c         1441 g          741 t
ORIGIN      
        1 agacgactgc agcacgtcag tgaatgtcgg cgtggtgtcg gtcgaggatc cagcggttgt
       61 accgaacgct gcagtgaacg gcccagaggc ggcaacggtc ggcgctgcgc tgtatccgat
      121 ggcgccgttt gtcgttccgc aactcgcacc agcaccggcg agctgcaccg tgatggtgtc
      181 gccgatgtgg aagttgttcg gcaagccgat gttgaagtcg ccgagggctg ccgcggtggt
      241 gccggggttg accgtggtcg cagcggtcgc accaacttgg gtgcttaccg ctagttcggc
      301 cgcgaatgcg tttgtggcgg tgccaataag catcgctcct gccgttacgg ctgccgcgaa
      361 gcccgcgacc cccgtacgac gcaaccgtct ggttgctgtt gtcactcttg cctccatcag
      421 agaagtggtg gtggcgaggt ggctcagccc gaaggcgcaa taccgaatgc cccgccgata
      481 cccgagcaga ctgctcgatt tgggacctta ccggttttgc gctcacggaa gccagacgcc
      541 ccacccacga cttacgtcgt gtcgtggttt gggttaccaa accggacatt cgtccgatct
      601 agcttcctga atctaccgac tcttgaccat gggcgagaca agggaggcat aagcctcgag
      661 gtggcttctg cctacctcaa ttgaagagtg tcttaacgtt gccacccctc tcggggagcc
      721 aaacgggagc cttgccagcg cggcggcgag cgcgatcggg tcctcgggct ccactaaata
      781 gccgtcgacg ccatcttcca cgctctcagc caggccaccg acacggctag cgaccaccgg
      841 gcggccgcgg gtgaacgcct caagcgccac catgccaaga ccctcacgcc gcgaaggcgc
      901 cacgagcgcg tggactcctt cgagccggtg catcagctcg gcgtgtggga gcgcagggat
      961 gagctggatc cgatcgccga gcggcgcggc taataaagca agacgacgac gctcgggacc
     1021 tgagccgaca atgtcgaggc ggacgtcgag gccgctgccc accgcgatcc gcatcgcttc
     1081 gatgaggacg tcgaagccct tctcatgcga aagccgcccg gccgcaagca atcgcagcgg
     1141 cggatcggcg ggcatcggcg ccgcggccgg tccggaaggc accggcaggc gcaaaacggc
     1201 ggtgtgcagg cgcggatcga actccaggat ctgccgctgc agatcctccg acaccgttgc
     1261 caccagcgac gcacggtgca tgacgtggga cgcgagcttg cgcaacgcgc ggtagcgcag
     1321 caggtgaacg tcggagccgt gcacagtgat gatcagcggg acgtcgaggc gccgggccac
     1381 cggcaccgcg cacaacccgg cgggcaacca ccagtgcgca tggatgacgt cgggccggaa
     1441 gtcgcgggcg gccttctcag cagccctcga gaacgacgcc agataggccg gcaacatgat
     1501 gccgctgaac cgccgcgaac tgccgagcaa gccgccgcga tacgacagct tttccataga
     1561 ttgcaggccg taacgaaaac ggtgaacctc gacgccggca actgtgtcga cggtggcgag
     1621 ccccttgtcg tggggcgcca gcaccccgac gtccatacct gcttcttgtt ctgcgtgcgc
     1681 aaggtcaaga agaaatggcg cgacgtggtc ccgcgcatcg cgcgggaacg aggaggtcaa
     1741 atgaagcacg cgcacgctcg tagcgtaacg gtccgagcac gtaatgtccg agtactggcg
     1801 tgtgggtcgg tgaagcgtta aagtcccgcg ccgctagttg agcgttcagt cgccggaaac
     1861 agcccttgtg taagcaacta aatgcgcctg cgcgctggct tcccaggtga attcgagcga
     1921 ccgcgcatgt gccgcgctac ccagcgcacg acggcgcggc tcatcggcca gcaaccccga
     1981 cagcgccgtc ccgattgaag cggagtcagg ctccgtgtag gcgaccgcat cgccgccgac
     2041 ttcgggcaac gacaaacggt gcgtggtgag caccgcggcg ccgcacgcca tcgcttcgag
     2101 caccggcagc ccgaaaccct cgccgtggct gggataggcg accacgagag ccccgccgag
     2161 gaacgccgag aggtcagcca tccgcaagta gccgggtctg atcacccgca ggtgcgaggg
     2221 cacttcggcg acagcttctt cgatctcgtc gtcccagccc gcgccgccgg cgatgacgag
     2281 cgcgggtggc gcatcccact ggccgaccgc gcttacccag ccccggatca ggctcggcac
     2341 gttcttgcgc ggctcgaaag cgccgagaaa agcgatgtat tgctggccgc gcaggccaag
     2401 gcgcgccgag atgcgcgcgg cctcagcctc tgtcggcggc ctgaacacct cgtggtcgac
     2461 gccgtggtac gcgacgtcga tgcgagtcga gtcggcatca acgaggcgga cgagctcgtc
     2521 gcgtgttgcc ttcgacggca cgatgcagcg ggttgatcgc ttcaccgccg agcgaatcgc
     2581 ggcgcggtag aacgcgccac gcgtgggcgg gtaaagatcg ggctcgctga agaacgtggc
     2641 gtcgtggatc gtggtgacca ctgggcggcc aggccgcatc ggcactgaat aattcggcgc
     2701 gtgaataact ttcgccgcca cgtgttcggc gaccagtggc agcccggtct gctcccacgc
     2761 gatgcgcgcg gtgcggttcg tgagcgaggc cggcccggct acgatctgcg cggcgggcac
     2821 cagccgggcg taccgctcgg cgtcgacccg ctggcacacc acggcaaggt cggcgtcctt
     2881 cgccgcaagc gctgcgagca gaccgtcgac gtatctcccg aggccacccc ggtcagcggg
     2941 aacagcactg gcgtcgatga gaacccgtgg cgccacgatg cagtccacct cgaacgttca
     3001 cgtcggatcg atgcttcgga ccgtcatggt ccctggcagt agtcgtgtta gttgtcactg
     3061 ttgcagttgg aagcttactg ccgcgagggc ggtagccagt tgtaggaagg atggtgagaa
     3121 tggtcgggtc ggccgcgaca gacaggtccg tcgtcgcaac cgtcgtggtc gtcgcctgga
     3181 acggcgctga actcatcggc ccctgcctcg acgctctcgt ggagcaggag ttggccgacg
     3241 gattcgtgac ctgggtgatc gacaacgcat cgaccgacag cactgggagc gttgtcagac
     3301 aacaccccgt cggcgcccga ctggtgtctg gcgcccgcaa tctcggcttt gccggtgccg
     3361 ctgaacttgc cgtgcgtgag gcaacgacgc cacttgtcgt cgtcctgaat caagatgcgc
     3421 gccccgaacc gggctggctc gctgcattgc tcgcgccgtt gacaggtccg gacgcacatc
     3481 gcatcgccgc cgtcaccagc aaggtgatct tcaccgccga cggccgactg aacaacaccg
     3541 gcgtgatcgt gggccgtgac ggttacggtc gcgatcgtgg gttcggccag ccggacgacg
     3601 gtaggttcgc gtcggcggaa gacgtcttcg ccttttctgg caccgcggca gcgttacgag
     3661 tggtggcagc tcgcgacgtg ggctcgttcg atccatcgtt ctttctctac tacgaggaca
     3721 ccgatctctc gtggcgcctt caactcggtg gttggcgagt gcgctacgcg ccggacgcga
     3781 tcgtgcatca cgtgcacgcc gcgtcgaccg acgtgtcatc tgcagctttc gccttttaca
     3841 acgagcgcaa ccgactgctc atgttggcaa agtgcgcgcc tcgaaccctt gccgttaaag
     3901 aggttctgcg cttcgcctac atcacgtttg cgttgccgtt gcggcgcgtt atgcgtgtcg
     3961 acgttccgac gggtcatcag ttccgcacag ctacccgcgt gcgcgcgctc gcatcgttcg
     4021 cgcgcatgct gccggctgtg ctccagaaac gccgcgccgt ctcgcggatc tgccgacgct
     4081 cgcgcgcaga agtagcccgc gaactctcct agttccgccg cacactcttg cactgacgcg
     4141 attccgccga cccattccgc gagtactagg gccggtcgtg agctacgagg tgttgaccga
     4201 cgtgcagccg agcgacgtgt tcgtgtcgcc ccggccgacg ttgataccga atgcacagac
     4261 tgtgtgcttg ccagccttga cgcccaccgg gaacgcgaac ccgtgcgcgg caccgaagct
     4321 cggatagtcc gccgcaacgt cgggtcgcga gatcgaagcg gtctgctgca gcgcaacact
     4381 gccgtcgac
//
LOCUS       DASWVF010000400         9343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_35949, whole genome shotgun sequence.
ACCESSION   DASWVF010000400 DASWVF010000000
VERSION     DASWVF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35949"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGD55_07195"
     CDS             complement(<1..414)
                     /locus_tag="VGD55_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0870167.1"
                     /translation="MKIVIAGSSGLIGSALVRVLRSEGDGQAHQVVRLVRREATADDE
                     IAWDPSAGRGPDLAALKGVDAAINLAGVGVGDHRWTDDYKWQILQSRVSSTELLVAAL
                     RQLEPQPRVLLSGSAIGYYGETGDTAVDEDSHAGND"
     gene            complement(411..1871)
                     /gene="sucB"
                     /locus_tag="VGD55_07200"
     CDS             complement(411..1871)
                     /gene="sucB"
                     /locus_tag="VGD55_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEY0870168.1"
                     /translation="MSVSVTMPRLGESVTEGTVTRWLKKEGDQVAVDEPLLEVSTDKV
                     DTEIPSPVAGILSKITVAEDETVEVGSELAVIDDGSANAAPTAPATQQTTTQPDAEVP
                     APPAPAPAPTPPPPPAPPAAAAPTPPPAAPIPVPVQPAPAPAQFSAAPPATAVAAATV
                     LPVQSQDDGGGDPSYVTPLVRKLATEHGVDLGALSGTGVGGRIRKQDVLAAAEAARVA
                     KAAAASNGSASAARPAAAAATASPAAAALRGRTEKMSRLRGVLAARLVESLQTAAQLT
                     TVVEVDITRVARLREATKAEFEAREGVKLSFLPFFALAAVEALKQHPVLNASIDTAAG
                     TITYHDAEHLGIAVDTERGLLVPVVRDAGDLNLGGLARKIADVANRTRNNQVNPDELS
                     GGTFTLTNTGSRGALFDTPILLPPQVGILGTGAVVKRPVVIDDADLGETISVRSMVYL
                     ALTYDHRLVDGADAARFLGTIKARLEEGRFEADLGL"
     gene            complement(1911..3293)
                     /gene="lpdA"
                     /locus_tag="VGD55_07205"
     CDS             complement(1911..3293)
                     /gene="lpdA"
                     /locus_tag="VGD55_07205"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0870169.1"
                     /translation="MANENGTFDLVILGGGSGGYAAAFRASELGLAVALIEKDKVGGT
                     CLHRGCIPTKALLHAAEIADGAREGASIGVRSTFEGIDVPAVHAYKDGVVNRLYKGLQ
                     GLVKANKITYVEGGGKLVSPTAVEVNGQRYEGRHILLSTGSVPRSLPGLDIDGDRVIS
                     SDHALRLDRVPASVVVLGGGVIGVEFASVWKSFGADVTIVEALPHLLPLEDEASSKLL
                     ERAYRRRGINFELGARFESVKTTDAGVVVTLEGGKTIEAEYLLVAVGRGPVSAGLGYE
                     ENGVATDRGFVLVDEYCLTNVPTISAVGDLIPTLQLAHVGFAEGILVAERLGGLSPVP
                     IDYDGVPRITYSEPEVASVGLTEAKAKEKYGDNLKSFTYDLAGNGRSQILKTSGAVKV
                     IAVADGPVVGIHIVGSRVGELIAEAQLIYNWEALPSEVAQLVHPHPTQTEAIGEAHMA
                     LAGKPLHVHS"
     gene            complement(3410..4936)
                     /locus_tag="VGD55_07210"
     CDS             complement(3410..4936)
                     /locus_tag="VGD55_07210"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0870170.1"
                     /translation="MTVIALAADSPAQIKVDAVVIGVAAGADGRPALAPGSQAIDAAL
                     RRRLVTALVSLGAAGDAGECYRIATLGATTAPVVVVVGLGASPGRGRQFDPEVLRRAA
                     GVALRSLAGTRKVAISLGTDTDESLAAVAEGALLGAYSYRRYRQASLEGHPAPVASVV
                     VLVADPKSRAARQVLTRAKIVCDAVCATRDLVNTPPSDLHPREFAEFAVAEATREGLD
                     IEVLDEKALKKGGYGGIIGVGQGSVNPPRLVRLAYRHPKATKTLALVGKGITFDSGGL
                     SLKPAAPMEWMKSDMGGAAAVLEAMVAIARLKLPVNVTGWAPLAENMPSGAAQRPSDV
                     LHIYGGKTVEVLNTDAEGRLVLADALARASEELPDYIVDAATLTGAQLVALGTRTSAI
                     MANDDDFRDAVADAANRAGEQMWPMPLPPELRKSLDSSVADIANMGESFGGMLVAGVF
                     LSEFVGKRDGKPITWAHLDIAGPAFNQGDAYGYTPKGGTGTAVRTFVQLADDVAAGRL
                     "
     gene            complement(5009..6517)
                     /locus_tag="VGD55_07215"
     CDS             complement(5009..6517)
                     /locus_tag="VGD55_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870171.1"
                     /translation="MSQNTTSTSSPAPASHESATHAHIPSWAVLAIACAAQFMVVLDI
                     SIVNVALPKMGQSLDMNQSELQWVVNAYALTFAGFLLLGGRAGDLFGRKKIFLLGLIV
                     FVGASLVGGFAQNGTMLEVARAAQGLGGAILAPSTLSLLTTTYTEQRERARALGLWSA
                     TAGSGGAFGVMAGGILTDELSWRWVLFVNVPIGAVLFAAAWVALRESRGQIRTIRSLD
                     IPGTITVTGGLAVLVYAIVSTNEHPWGSARTVVTLAIGVALLAAFVAIERYTPQPLVP
                     LRIFKMRALSSANGIAIAVGAVMFSMFFFLSLYMQDVLRFSALKTGFAFVPGALMIIL
                     GAQTASRLVSKVGPRRIIVIGGALGAVGMWWLSRLPATGHYYAHVLVPMMLVSLGLGL
                     SIVPVTVAATLGVDRSEAGLASGLVNTTRQVGGALGLAILATIATDRSKGLRTAGALS
                     SGFDRAFLVGGFIALLGALLALTLPSRNTHVAAVEQSARDHSGPPQPAVAEH"
     gene            6724..7872
                     /gene="gcvT"
                     /locus_tag="VGD55_07220"
     CDS             6724..7872
                     /gene="gcvT"
                     /locus_tag="VGD55_07220"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0870172.1"
                     /translation="MTDAPEHAASVGDLLRSPLHDRHIALGAKMGEFGGWTMPLEYAG
                     GGVIAEHTAVRERVGVFDVSHLGKASVRGPGAAAFIDATLTNALSRIEPGGAEYTLCC
                     DDATGGVVDDLIVYLRSDDDVFLVPNAANTAEVVRRLSAAAPSGVGVANLHRDFAVLA
                     VQGPRSPELVEALGLPADHPYMSFVDARWAPGAGAAIDVTVCRSGYTGERGYELVMRS
                     DDAGVVWDQLFEAGVALGVQACGLGARDTLRTEMGYPLHGQDLSLDITPNQARVGWAV
                     GWQKPAFWGRDVLLAERAAGPPRILRGLESLDRGIPRPHMVVNSATGDVVGEVTSGTF
                     SPTRRVGIGLALLKPDVVEGDEVQVDVRGRPSTMRVVKPPFIEGSRAG"
     gene            7869..8390
                     /locus_tag="VGD55_07225"
     CDS             7869..8390
                     /locus_tag="VGD55_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0870173.1"
                     /translation="MSGSDRVVLVGMMGSGKSTIGASLSKLTGWPYIDNDEVVEQMVG
                     MPTRDLLQQRGVAAMRTAESAAAQQVLTMKAPLIAGVAAGIVLDPAVSEQVHDGAFVV
                     FLRAHIDTLAKRVEGTYRPWLGDDPEATLRKLYEGREPLYEKLAHLVVDVDDAKPDDV
                     AHRILDTLNSAAQ"
     gene            complement(8400..>9343)
                     /locus_tag="VGD55_07230"
     CDS             complement(8400..>9343)
                     /locus_tag="VGD55_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870174.1"
                     /translation="QDPLAAAMANGRPSARDVAGRRGHAMTSIAATRARRRPAAVGLH
                     LRSTFALVVASGFGVLAFGWPFLLRSNGGNVNTAHTTDAPWIFAIVLPLLLMVVLAEI
                     ADGGIDAKAIALLGVLAACGGALRPLGIAATGASPVFFLLIPAGRVLGRGFGFVLGTL
                     TLFGSALLTGGVGPWLPFQMLAAGWVGLFAGCLPPLRGRLEIAMLAAYGAAAGMLYGA
                     VTNLWFWPFETSGLSPQISYVPRGNWLTEIHHYASFYVATSIGWDLSRALGTALLVVI
                     AGRPVLLALRRAARRAAFTAPVSFVPATPVVRVGQQG"
BASE COUNT         1592 a         3032 c         3222 g         1497 t
ORIGIN      
        1 gtcattgcca gcatgagaat cttcgtcgac tgcggtgtcg cccgtctcgc cgtagtaccc
       61 gatcgcggag cccgagagca gtacccgcgg ctgcggctcg agttgccgga gcgctgcgac
      121 gagaagttca gtcgacgaca cccggctctg gaggatctgc cacttgtagt cgtcagtcca
      181 gcggtggtca cccacgccga caccagcaag gttgatcgcc gcatcgacgc ccttgagcgc
      241 tgccaggtcg gggccgcgac cggcacttgg atcccacgcg atctcgtcgt cagcggtcgc
      301 ttcgcgtcgc accaatcgca cgacctgatg cgcctgcccg tcgccctcgc tgcgcagcac
      361 ccgcactaac gcggagccga tgagtccgga tgatccggcg atgacgatct tcataggccg
      421 aggtcggctt cgaagcggcc ttcctcgaga cgggctttga tcgtgccgag gaaccgggcc
      481 gcgtcggcgc cgtcgacgag tcggtggtcg taggtcagtg cgaggtagac catcgaccgc
      541 accgagatcg tctcgccgag gtcggcatca tcgatcacca cgggccgctt cacgacggca
      601 ccggtgccga gaatgcccac ttggggcggc agcaggatcg gcgtgtcgaa cagtgcgccg
      661 cggctgccgg tgttcgtcag cgtgaaggtt ccgccggaca gctcatcggg gttgacctgg
      721 ttgttacggg tgcggttggc gacgtcagcg atcttgcgcg cgagaccgcc gagattgagg
      781 tcacctgcgt cgcgaacgac cggaaccagc aagccgcgct cggtgtcgac ggcaatgcca
      841 aggtgctcgg catcgtggta ggtgatcgtg ccggcggccg tgtcgatgct cgcgttcaac
      901 accgggtgct gcttcaacgc ctcgacggcg gccagcgcga agaacggcag gaacgacagc
      961 ttgacgcctt cgcgcgcttc gaactcggcc ttggtggcct cgcgcagtcg cgcgacgcgg
     1021 gtgatgtcga cctcgacgac cgtggtcagt tgcgcggctg tctgcaacga ctcgacaaga
     1081 cgagcagcca ggacgccgcg gagccgggac atcttctcgg tacgaccgcg cagtgccgct
     1141 gcggcgggac ttgctgtcgc agccgctgct gccggtcgtg ccgcacttgc cgaaccgttc
     1201 gatgccgctg cggctttcgc gacccgggcc gcctcggctg cggcgagcac atcttgcttg
     1261 cggatgcgcc cgccgactcc ggtgccgctg agcgcaccga ggtcgacgcc gtgttcggtc
     1321 gccagcttgc ggacaagcgg cgtcacgtag gaggggtcgc caccgccgtc atcttgggat
     1381 tgcaccggca ggaccgtcgc ggctgcgacc gccgtcgccg ggggagcggc ggaaaactgt
     1441 gcgggcgcgg gggcaggctg cacgggcacc gggatcggcg cggcaggggg tggagtcggt
     1501 gcagctgcgg ccggcggcgc gggtggcgga ggtggagtcg gtgctggtgc tggtgcgggt
     1561 ggcgcaggca cctcggcgtc cggctgagtt gtcgtctgtt gtgttgcagg cgcagtcggc
     1621 gcagcgttcg ccgaaccgtc gtcgatcacg gcgagttcgg aaccgacctc gaccgtttcg
     1681 tcctcggcca ccgtgatctt gctgaggatc ccggccacgg gggacgggat ctcggtgtcg
     1741 accttgtcgg tcgacacctc gagcagcggc tcgtcgaccg cgacctggtc gccctctttc
     1801 ttcaaccagc gcgtgaccgt tccctcggtg acgctctccc cgaggcgggg catcgtgacg
     1861 gagacggaca tgtgggcgtt ggctcccggt tcctgaaggt gagagtggac ctagctgtga
     1921 acgtgcagcg gcttgccggc caatgccatg tgcgcttcgc cgatggcctc ggtctgggtc
     1981 gggtgcgggt gcaccaactg ggcgacttca ctgggcagcg cttcccagtt gtagatcagc
     2041 tgagcttcgg cgatgagctc accgacccgg ctgccgacga tgtggatgcc gacgacggga
     2101 ccgtcggcaa ccgcgatcac cttcactgcg cctgatgtct tgaggatctg gctgcggccg
     2161 ttaccggcga ggtcgtaggt gaagctcttg aggttgtcgc cgtacttctc cttggccttg
     2221 gcctcggtga gtcccaccga tgcaacctcg ggctcggagt aggtgatgcg cggcaccccg
     2281 tcgtagtcga tcggtaccgg ggagagcccg ccgagacgct cggcgaccag gatgccttcg
     2341 gcgaagccga cgtgcgcgag ctgcaaggtc gggatcaggt cgccgaccgc tgagatcgtt
     2401 ggcacgttgg tcaggcagta ctcgtcgacc agcacgaatc cccggtcggt ggcgacgccg
     2461 ttctcttcgt agccaaggcc ggcagatacg ggtccgcgac cgactgcgac caacaggtat
     2521 tcggcttcga tcgtcttgcc gccttcgagg gtgacgacca cgcccgcgtc ggtcgtcttg
     2581 acgctctcga acctggcgcc gagttcgaag ttgatgccac ggcggcgata ggcgcgctcg
     2641 agcagcttcg agctggcttc gtcctcgagc ggcagcaggt gcggcaacgc ctcgacgatg
     2701 gtgacgtccg cgccgaacga cttccagacg ctcgcgaact cgaccccgat cactccacca
     2761 ccaagcacca cgacggaggc cggaacccgg tcgaggcgaa gagcgtgatc gctgctgatg
     2821 acccggtcgc cgtcgatgtc gaggccgggc aaggagcggg gcaccgagcc ggtcgagagg
     2881 aggatgtggc ggccctcgta gcgctggccg ttgacctcga ctgcggtcgg tgagacgagc
     2941 ttcccgccgc cctcgacgta ggtgatcttg ttggccttca ccaggccctg caagcctttg
     3001 tagaggcggt tgaccacgcc gtccttgtac gcgtgcaccg ccggaacgtc gatgccctcg
     3061 aaggtcgacc tcacgccgat cgatgcacct tcacgggcgc cgtcggcgat ctcggctgcg
     3121 tggagcagcg ctttggtcgg gatgcagccg cggtgcaggc aggtgccgcc gaccttgtcc
     3181 ttctcgatca gcgcaacggc aaggccgagc tcgctggcgc gaaaggcggc cgcatagccg
     3241 ccgcttccac cgccaaggat caccaggtcg aaggtgccat tctcattcgc cacgaggact
     3301 cctgttggaa agggctgcgg tattcatcct cccacgccga cgcgaggcgc gggatcgggc
     3361 aacgttccgg cagcgttcgg gcagcgtgcg ggcaacgtac ggccaagagc tagagccggc
     3421 cggccgcgac atcgtcggcg agctggacga acgtgcggac ggcggtacca gtaccgccct
     3481 tcggcgtgta cccgtatgcg tcgccctggt tgaaggccgg accggcgatg tcgaggtgcg
     3541 cccaggtgat cggtttgcca tcgcgcttac ccacgaactc ggagaggaat acgccggcca
     3601 cgagcatgcc gccgaacgac tcgcccatgt tcgcgatgtc ggcgaccgag gagtcgaggg
     3661 acttgcgcag ttccggcgga agtggcattg gccacatttg ctcgcccgcg cgattcgcgg
     3721 cgtcggcgac cgcgtcgcgg aagtcgtcgt cgttggccat gatggccgag gtacgggtgc
     3781 cgagtgcgac gagttgggcg ccggtgaggg tcgcggcgtc gacgatgtag tcgggcagtt
     3841 cctcgctcgc ccgcgccaaa gcgtcggcga ggaccagccg gccctcggcg tcggtgttca
     3901 gcacctcgac ggtcttccca ccgtagatgt gcagcacgtc tgacggacgt tgcgcggcac
     3961 ccgagggcat gttctcggca agcggcgccc agccagtgac gttgacgggc aacttcaacc
     4021 gggcgatcgc gaccatcgcc tcgagcaccg cggcggcccc gcccatatcg gacttcatcc
     4081 actccatcgg cgcggccggc ttcagcgaga ggccgcccga atcgaaggtg atccccttgc
     4141 cgaccaacgc caacgtcttc gttgccttcg ggtgccggta cgcgaggcgc acgagtcgcg
     4201 gcggattcac cgagccctga ccgacgccga tgatgccgcc gtagccgccc ttcttcagcg
     4261 ccttctcgtc gagaacctcg atatcgaggc cttcccgcgt cgcttcagcg acagcaaatt
     4321 cggcgaactc tcgtgggtgc agatcggacg gcggggtgtt gacgagatcg cgcgtcgcgc
     4381 agaccgcatc gcacacgatc ttggctcgcg tcagcacctg acgtgccgcg cgtgatttcg
     4441 ggtcggcgac gaggacgacc acagaggcga ccggtgccgg atggccctcg agcgaggcct
     4501 ggcggtaacg ccggtaggag tacgcgccga gcagtgcacc ctcggcgact gcggccaacg
     4561 attcgtcggt atcggtgccg agcgagatcg cgaccttgcg agtaccggct agtgatcgca
     4621 gcgcaacccc ggccgcccgt cgcaacactt ctgggtcgaa ctgccgccca cgaccagggg
     4681 atgcacctag gccgaccacc acgacaaccg gagccgtggt ggcgccgagg gtcgcgatgc
     4741 gatagcactc acccgcgtct ccggcggcgc cgaggctgac gagcgcagtg acgagacgtc
     4801 ggcgcagggc cgcgtcgatg gcctgcgagc caggcgcgag cgccgggcgc ccgtcggctc
     4861 cggcggctac gccgatgacg actgcatcga ccttgatctg ggcaggggag tcggcagcga
     4921 gcgcgatgac agtcacggcc gccgatcgta tagggacgcg gagctcaccg tgcccagcaa
     4981 cgaccgacta atgctcagca acgaccggct agtgctcagc gaccgcgggt tgcggtggtc
     5041 cgctgtggtc gcgtgcgctt tgctcgacgg ctgccacatg ggtgttgcgg ctcggcaggg
     5101 tgagggcgag gagcgctccg agcagggcga tgaatccacc gacgaggaac gcgcggtcga
     5161 atcccgaact cagggcgccg gcagttcgaa ggcccttgga gcggtcggtc gcgatcgtcg
     5221 cgagtatcgc caggccgagc gctccaccga cctgccgggt ggtgttgacc aacccggacg
     5281 cgaggcccgc ttcactgcgg tcgacgccca acgtcgccgc gaccgttacc ggcacgatgg
     5341 aaagcccaag tccaagagac accaacatca tcggcaccag cacgtgggcg tagtagtgac
     5401 cggtcgcagg cagccgtgat agccaccaca tgccgacagc gcctagcgcc cctccgatca
     5461 cgatgatgcg gcgaggaccc accttgctta ccagtcgcga tgccgtctgc gccccgagga
     5521 tgatcatgag cgcgccggga acgaacgcga acccagtctt caacgcgctg aagcgaagaa
     5581 catcctgcat gtagagcgac aggaagaaga acatcgagaa cataactgca ccgaccgcga
     5641 tagcgatgcc gttggccgat gacagcgcgc gcatcttgaa gatgcgtagc ggtaccagtg
     5701 gttgtggcgt gtagcgctcg atggcgacga acgcggcgag caacgcaacc ccgatagcga
     5761 gggtcaccac cgtgcgggca gagccccacg ggtgctcgtt ggtgctgacg atcgcgtata
     5821 cgagcacggc caggccaccg gtcacggtga tggtgccggg gatgtcgaga gagcgaatcg
     5881 ttcggatctg accacgcgac tcgcgcaggg cgacccaggc cgcggcgaac aggacggcac
     5941 cgatcggcac gttcacgaac agcacccacc gccagctcag ctcgtcggtg agaatgcctc
     6001 cggccatgac gccgaacgcc ccaccgctgc cagccgtcgc actccacagg ccgagcgcgc
     6061 gagcgcgctc gcgttgctcg gtgtaggtcg tggtcagcaa gctcagcgtc gatggagcca
     6121 agatggcgcc gccaagaccc tgcgccgcac gtgcgacctc aagcatcgtc ccattctgcg
     6181 cgaagccgcc gaccaggctt gcgccgacga agacgatcag accgaggagg aagatcttct
     6241 tgcgaccaaa caggtcacct gcgcgaccgc cgagcagtag gaagcccgcg aacgtcagcg
     6301 cgtaggcatt gacgacccat tgcagctcgc tctgattcat gtccagcgac tgacccatct
     6361 tgggcaatgc cacattcacg atcgagatgt cgagaacgac catgaattgc gcggcgcagg
     6421 cgatggcgag taccgcccac gacgggatgt gcgcgtgggt ggctgactcg tgtgaagcgg
     6481 gagcaggcga agaggtgctg gtcgtgttct gactcatcgg taacccctga tgtgcggctg
     6541 gctacagcgg gcgggcgtgt acgggcacag agccgacttc ctcgactcta ggcccaaacc
     6601 tacgaacgcc ctgagacatt tccgccggga gggtgcggcg cacgatgacc tgcggtgcgc
     6661 gagggagact gcgcgaggga gatggagggc gtgcccgagg cgcggaagcg gtaacgtccg
     6721 cgcatgacag atgcccctga gcacgccgcc agcgtcggcg acctgctccg ctcgccgctc
     6781 cacgaccgcc acatcgcgct cggagcaaag atgggcgagt tcggcggctg gaccatgccc
     6841 ctcgaatacg ccggcggggg agtgatcgcc gagcacaccg cggtccgcga acgggtcggt
     6901 gttttcgacg tctcgcatct cggcaaggcc tcggtgcgcg gccccggagc agcagccttc
     6961 atcgacgcca cgctgacgaa cgccctgtcg cgaatcgaac ccgggggggc cgaatacacg
     7021 ctgtgttgcg acgacgccac cggtggcgtc gtcgacgacc tcatcgtcta cctgcgctcc
     7081 gacgacgacg tcttcctcgt gcccaatgca gcaaacaccg ccgaggtcgt gcgccggctg
     7141 tccgccgcgg cgccgtcggg cgtcggcgta gcgaatctgc atcgcgattt cgcagtgctg
     7201 gccgtgcagg gtccgcgctc acccgaattg gtggaggcgt tggggttgcc cgcggaccac
     7261 ccgtacatgt cgttcgtcga tgcacgatgg gcgccggggg ccggtgccgc gatcgacgtg
     7321 acggtttgcc ggtcgggcta caccggtgag cgtggctacg aactcgtgat gcgcagcgac
     7381 gacgccggcg tcgtatggga tcagctgttc gaggcggggg ttgccctcgg cgtccaggca
     7441 tgcggtcttg gtgctcgtga caccctgcga accgagatgg ggtacccgtt gcacgggcag
     7501 gacctttctt tggacatcac gccgaatcag gccagggttg gctgggccgt ggggtggcag
     7561 aagccggcgt tctgggggcg cgacgtcttg ctggccgaac gtgcggccgg accgccgcgc
     7621 atcctgcgcg gacttgagtc gctcgaccgt ggaattccgc gcccgcacat ggtcgtgaac
     7681 tcggcgacag gtgatgtggt cggcgaagtg acgagcggta ccttctcgcc gactcgtcgg
     7741 gtcggtatcg ggcttgcctt gctgaagccg gacgttgtcg agggcgacga ggtgcaggtt
     7801 gacgtccggg gccgaccctc gacgatgcgg gtcgtcaagc caccgtttat cgaggggagc
     7861 cgtgcggggt gagcgggtca gatcgtgttg tgcttgtagg gatgatgggg tcgggcaagt
     7921 cgacgatcgg cgcaagcctg tcgaagctca cgggttggcc ctacatcgat aacgacgaag
     7981 tcgtcgagca gatggtcggc atgcctacgc gtgacctgct tcaacaacgt ggggtcgcgg
     8041 cgatgcgcac cgccgaatct gctgctgcgc aacaggttct gacgatgaag gcaccgttga
     8101 tcgcaggtgt cgccgctggc atcgtgctcg atcccgctgt cagcgagcag gttcacgacg
     8161 gagcgttcgt cgtcttctta cgcgcgcaca tcgacacgtt ggcgaaacgg gtcgagggta
     8221 cctacaggcc gtggctcggc gatgatcctg aggcgacgct gcgcaagctc tacgaaggcc
     8281 gagagccgct gtacgagaag ctcgcacacc tggttgtcga cgtcgatgat gcgaagccgg
     8341 acgacgtagc gcaccggatc ctcgacacat tgaactctgc tgcgcaatga cctccatcgt
     8401 cagccctgct gcccgactcg cacgacgggc gttgcgggca cgaacgacac aggagccgtg
     8461 aacgccgcgc gtcgtgctgc ccgtcgcagt gcaagcaaca ccgggcggcc agcgatgacg
     8521 acgagcaggg cagtgccgag agcccggctc agatcccatc cgatcgacgt cgcgacgtag
     8581 aacgaggcgt agtggtgaat ctcggtgagc cagtttcccc gtggtacgta agagatttgt
     8641 ggtgagagcc cactggtttc gaacggccag aaccacaagt ttgtgaccgc tccgtagagc
     8701 atgccggcag ccgcgccata cgcagcgagc atcgcgatct cgaggcgccc gcgcagcggt
     8761 ggcaagcagc ctgcgaagag cccgacccag ccggccgcca gcatctggaa cggcagccac
     8821 ggccccaccc cgcccgttag cagggcgctg ccgaacaacg tgagggtgcc gagcacaaat
     8881 ccgaaacccc gcccgagcac gcgcccggcc gggatgagca agaagaacac cggcgatgct
     8941 cctgtcgctg cgatgccgag aggccgcagg gcacccccgc acgcggccag aaccccaagc
     9001 agcgcaatgg ctttcgcgtc gatgccgcca tcagcgatct cggcgaggac gaccatcagc
     9061 agcagcggca acacgatcgc gaagatccac ggcgcgtcgg tggtgtgcgc cgtattgacg
     9121 ttcccgccgt tgctgcgcag caagaacggc cagccgaaag ccagcactcc gaacccgctt
     9181 gccaccacca acgcgaacgt cgaacgaaga tgcaacccaa cggcggccgg acgccgacgg
     9241 gcgcgcgtcg ccgcgatgct cgtcatggcg tgacctctgc ggccagcaac gtcgcgcgca
     9301 cttgggcgac cgttagccat ggcagcggcg agaggatctt ggc
//
LOCUS       DASWVF010000401         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_494591, whole genome shotgun sequence.
ACCESSION   DASWVF010000401 DASWVF010000000
VERSION     DASWVF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494591"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGD55_07235"
     CDS             <1..791
                     /locus_tag="VGD55_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY0870175.1"
                     /translation="VGAGSQPDFYIGWLEGTLRMMPNLETHVDGHTISWNVLIPAVVI
                     PGILFTAMALYPFIEAWVTRDYETHNICDRPRNRPGRTALGVAVLTELGVCLLAGGND
                     VLAEKYDISLYATTWTFRVAFFVLPPLAFMATRRICLGLQQRDLHSAQHGYETGRIVM
                     LANGEFIEVEAALPEDAIARMGLEYRQGPPPALEAPVDEHGVRSKSRHNPIEKVRNSI
                     GAFLYEPDTEPLTNGHGNGHGTNGHGDAELPAHDQPELTSGSTD"
     gene            829..1197
                     /locus_tag="VGD55_07240"
     CDS             829..1197
                     /locus_tag="VGD55_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY0870176.1"
                     /translation="MPKRAGVDDYFAQLSDVQRPHLEMLRKLSFKADPKAREVLKWNL
                     PVYIRGENTNLWMLQNFKNHCSLRFSPPFFATHKAAVEAAGYEAGAGFIKLPYTRALP
                     TKLLKTLMHARVEEFETGGR"
     gene            complement(1194..1574)
                     /locus_tag="VGD55_07245"
     CDS             complement(1194..1574)
                     /locus_tag="VGD55_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0870177.1"
                     /translation="MRIDRLDHLVLTVADLDATIEFYVGVLGMTDTVFGDGRHALTFG
                     ASKINLHLAGHEFDPRALHPQPGSADLCFIVDDQIEAIAAALTSAGVAIEEGPVNRTG
                     ATGPIRSLYVRDPDHNLVELSNYL"
     gene            complement(1598..1993)
                     /locus_tag="VGD55_07250"
     CDS             complement(1598..1993)
                     /locus_tag="VGD55_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY0870178.1"
                     /translation="MKTEGLLFAGCAFFFAVVDAVYWTLSHDPTGTTALALAVGLAFL
                     VGFYLFVTGRRTGLRPEDRSDADISESAGEFGFFSPHSWWPLFTAASVALVALGVVFA
                     WWLAILGMMFVILTAFGFVLEYYRGIHIP"
     gene            complement(1993..>3606)
                     /gene="ctaD"
                     /locus_tag="VGD55_07255"
     CDS             complement(1993..>3606)
                     /gene="ctaD"
                     /locus_tag="VGD55_07255"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0870179.1"
                     /translation="MTTTDHKLIGQLYLATSFLFFLLAGVMVLLMRAELARPGLQLLT
                     NEQYNQLFTMHGTIMLLLFATPLFIGFANVIVPLQIGAPDVAFPRLNIFSFYLFLFGG
                     LITIGGFLTPGGAADFGWYAYTPLTNAAHSPGVGGDMWIMGLVVSGLGTILGGVNFVT
                     TIMVMRAPGMTMFRMPVFTWNILLTSMLVLLAFPVLAAALLCLEAERKLGAQIFTTAN
                     GGPILWQHLFWFFGHPEVYIVAIPFFGIITEILPVFARKPVFGYKGLVFATISISGLS
                     MTVWAHHMFSTGAVLLPFFSFMTFLIAVPTGVKFFNWIGTLWRGQLSFDTPLLWALGF
                     LVTFLFGGLTGIILASPPLDWHVTDTYFVVAHFHYVLFGTVVFAMFGGFYFWWPKMTG
                     KMLDERLGKVHFWTLFIGFHTTFLVQHWLGVIGMPRRIPDYLPSDGFTTLNTISTLGA
                     FLLGLSMFPFMFNVWKTWRHAPLVGVDDPWGWGASLEWATSCPPPRHNFVRMPRIRSE
                     RPAFDLHHPELIGPARVPVPAGAAEGGGV"
BASE COUNT          792 a         1127 c         1139 g          548 t
ORIGIN      
        1 aggtcggcgc cggatcgcag cccgacttct atatcggctg gctcgagggc acgctgcgga
       61 tgatgccgaa cctcgagaca catgtcgacg gtcacaccat cagctggaac gtgctgatcc
      121 cagctgtcgt catccccggc atcttgttca cggcgatggc gttgtacccg ttcatcgaag
      181 cctgggtcac ccgcgactac gagacccaca acatctgcga ccggccacgc aaccggcccg
      241 gtcgcaccgc cctcggcgtc gccgtgctga ccgaactcgg tgtctgcctc ctcgcaggtg
      301 gcaacgacgt tctcgccgaa aagtacgaca tcagcctgta cgcaacaacg tggaccttcc
      361 gcgtcgcgtt cttcgtgctg ccgcccctcg cgttcatggc aactcggcgc atctgcctcg
      421 ggttgcagca acgcgacctg cacagcgcgc agcacggata cgagactggc cgcatcgtga
      481 tgctcgccaa cggcgagttc atcgaggtcg aggcagcgct gccagaagac gcgatcgcgc
      541 gcatgggcct tgagtaccgg caaggtccgc cgcctgcgct cgaagctccc gtcgacgagc
      601 atggcgtgcg gtcgaagtca cggcacaacc cgatcgagaa ggtgcgcaat agcatcggcg
      661 cgttcctcta cgagccagat accgaaccgc tcaccaacgg gcacggcaac ggccacggaa
      721 caaacggcca cggggacgcc gaattgccgg cgcacgacca acccgaactg acctcaggct
      781 caaccgacta ggtgctgcgt tcagcagggg tacgctgacg ccgtgccaat gcccaagcgc
      841 gcaggtgtcg atgactactt cgcgcaactg agcgacgtgc agaggccgca cctcgagatg
      901 cttcggaagc tcagcttcaa agccgacccg aaggctcggg aggtactgaa atggaaccta
      961 cccgtctaca tccgcggcga gaacacgaac ctgtggatgc tccagaactt caagaaccac
     1021 tgctcgctgc gattctcgcc gccgttcttc gcgacccaca aggccgccgt tgaggccgcc
     1081 ggttacgagg ctggcgcggg cttcatcaag ctgccctaca ctcgcgcgct gccgacgaaa
     1141 ctcctgaaga cactcatgca tgcgcgggtc gaggaattcg aaaccggcgg tcgctagagg
     1201 tagttcgaga gttcgaccag gttgtggtct ggatcgcgaa cgtagaggct gcggatcggc
     1261 ccagtcgcgc cagtgcggtt cacggggcct tcctcgatcg cgacaccggc cgacgtcagg
     1321 gcggcggcta tcgcctcgat ctgatcgtcg acgatgaagc acaagtctgc agatccaggc
     1381 tgagggtgca gcgccctcgg atcgaactcg tgacctgcta ggtgcaggtt gatcttgctc
     1441 gcaccgaaag tcagcgcgtg gcgtccgtcg ccgaagacgg tgtccgtcat tcccagcacg
     1501 ccgacgtaga actcgatggt cgcgtcgaga tcggcgaccg tgagaacgag atggtcaagg
     1561 cgatcgattc gcatagctcg gcctaggttg tctccggcta cgggatgtgg atgccgcggt
     1621 agtactcgag aacgaagccg aacgcggtca ggatcacgaa catcatgccg agaatcgcta
     1681 gccaccaagc gaaaacgacg ccgagtgcga cgagtgcgac tgaagcggcg gtgaacaacg
     1741 gccaccaact gtgcgggctg aagaacccga actcccctgc ggactcagag atgtcggcgt
     1801 cgctgcggtc ttctggccgc aagcccgtac ggcgaccagt cacgaacaag tagaagccca
     1861 cgaggaacgc gagcccgacc gctaacgcca acgcggtcgt gccggtcggg tcgtgcgaca
     1921 acgtccagta gacagcgtcg accaccgcga agaagaacgc acacccggcg aagagcagac
     1981 cttcggtctt catcagacgc cgccgccttc agccgcacct gccggcacgg gcacgcgcgc
     2041 cgggccgatc agctcaggat gatgcaggtc gaacgccggc cgctcggagc ggatacgtgg
     2101 catgcgaacg aagttgtgcc gcggcggcgg gcacgaggtt gcccactcaa gtgacgcgcc
     2161 ccagccccac ggatcgtcga ccccgaccag cggcgcatgc cgccaggttt tccagacgtt
     2221 gaacatgaac gggaacatcg acaacccgag caggaaagcg ccgagcgtcg agatcgtgtt
     2281 cagcgtggta aacccgtcgc ttggtaagta gtcgggaatc cggcgtggca tgccgatgac
     2341 accgagccag tgctgcacga ggaatgtggt gtggaagccg atgaacaggg tccagaagtg
     2401 caccttgccc agacgctcgt cgagcatctt gccggtcatc ttcggccacc agaaatagaa
     2461 tccgccgaac atcgcgaaca ccacggtgcc gaacagcacg tagtggaagt gcgcgaccac
     2521 gaagtacgtg tcagtgacgt gccagtcgag cggcggcgac gccaagatga tgccggtaag
     2581 gccgccgaac aagaacgtca cgagaaagcc cagtgcccac agcaatggcg tgtcgaagct
     2641 cagctggccg cgccacagcg tgccgatcca gttgaagaac ttcacgccag tcgggacggc
     2701 gatcaggaac gtcatgaatg agaagaacgg caacagcacc gcaccggtcg agaacatgtg
     2761 gtgtgcccac acggtcatcg ataagccgga gatcgagatc gtcgcaaaga ccaatccctt
     2821 gtagccgaac accggcttgc gcgcgaacac cggcaggatc tcggtgatga tcccgaagaa
     2881 tgggatcgcc acgatgtaga cctcgggatg gccgaagaac cagaacaggt gttgccacag
     2941 gatcggaccg ccgttggcgg tcgtgaagat ctgcgccccg agtttgcgtt cggcctcaag
     3001 acacaacaac gcggcggcca acacggggaa cgcgagcagc accagcatcg aggtcagcag
     3061 gatgttccag gtaaagaccg gcatgcggaa catcgtcatg ccgggcgccc gcatcaccat
     3121 gatcgtggtg acgaagttga cgccgccgag gatcgtgccg aggccggaga cgaccagacc
     3181 catgatccac atgtcgccgc cgacgccggg cgagtgtgcc gcgttggtca gtggcgtgta
     3241 ggcgtaccag ccgaagtcgg cggccccacc cggcgtgagg aagccgccga tggtgatgag
     3301 accgccgaag aggaacaggt agaagctgaa tatgttcaac cgcggaaacg cgacgtcggg
     3361 tgcgccgatc tgaagcggca cgatgacgtt ggcgaacccg atgaacaacg gcgtcgcgaa
     3421 cagcagcaac atgatcgtgc cgtgcatcgt gaacagctgg ttgtactgct cgttcgtcag
     3481 cagctgaagc ccaggccgag ctagttcggc gcgcatcaac agcaccatga cgccagccag
     3541 caagaagaac aggaacgagg tcgcgaggta cagctggccg atgagcttgt ggtcggtggt
     3601 ggtcat
//
LOCUS       DASWVF010000402         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_265500, whole genome shotgun sequence.
ACCESSION   DASWVF010000402 DASWVF010000000
VERSION     DASWVF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265500"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VGD55_07260"
     CDS             <1..470
                     /locus_tag="VGD55_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870180.1"
                     /translation="SREFRHRPQSALNELTRRQHTALVTWSAFTATFIGVRGITYAIK
                     DNVGPFHNVTLGGEHIHHYMWGIGMVTLAGGLALQDRDAGDAKEVLAAIYGSGAALIV
                     DEFAELLDLQDVYWAKQGRLSVDVAIAIISISGMYLAARPFWHRLVKGQLKAT"
     gene            529..1905
                     /locus_tag="VGD55_07265"
     CDS             529..1905
                     /locus_tag="VGD55_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY0870181.1"
                     /translation="MTTDWGVALAEIDLPEFELPRTKPVIPAATYLQRIDALYQKMAD
                     RGLDAVVIYGDKEHFANTSYLSGFDPRWEESLLVFAPGRTPTLVVGNENVAWARVVPY
                     DINVVRVAKFSLIGQPDPGDVQLADVLRDAGITRGHVGLIGWKFWTGSAEHWIDVPHF
                     IVTEVAKLAGTVQNATDILMNPDGGLRIDNDVDQIAAFEFGASHASAAVNRLLHGIAP
                     GMTELEATQLMNPILLPFSYHPTMLSGPNAVYGVASAGDRVLEIGDPVCAGLGYWGGN
                     TARAGFLVHDASQLPAAASDYLEKMAAPYYATAAAWYETIRIGLTAGELYDVTFSRIG
                     DPFFGVYLNPGHFIHLDEWPSSPVAAGSPVVFKSGNAIQLDIIPITGSGYHTAQIEDG
                     VVLADSSLRASIAAKYPDVWARMQQRRTMLSKTFGIELHEEVLPLSNTAGYFPPFWLA
                     PPLAMVRT"
BASE COUNT          355 a          742 c          662 g          359 t
ORIGIN      
        1 tgtcgcgcga gttcaggcac cgaccccagt ccgcgctgaa cgagctcaca cgtcggcagc
       61 acaccgcgct ggttacctgg agcgcgttca cggcaacgtt catcggggtg cgcggtatca
      121 cctacgccat caaggacaac gtcggcccgt tccacaacgt caccctcggc ggcgagcaca
      181 tccaccacta catgtggggc atcggcatgg tcaccctcgc cggcggactg gcgttgcaag
      241 accgcgacgc cggcgacgcg aaagaggtgc tcgccgcgat ctacggcagc ggcgccgcgc
      301 tgatcgtcga tgagttcgcc gagctcctcg acctccagga cgtctactgg gccaagcaag
      361 gccggctcag cgtcgatgtc gccatcgcca tcatctcgat cagcgggatg tacctcgccg
      421 cccgcccgtt ttggcaccgc ttggtcaaag ggcagctgaa agccacctag ccgggacgcc
      481 gacgtgctgg cgccgaccgc ttagcgcccg ccccgatagc gtggcgacat gaccacagac
      541 tggggcgttg cgctcgccga aatcgatctc cctgagttcg agctgccgcg taccaagccg
      601 gtgattcccg cggcgactta cttgcagcgc atcgacgccc tgtaccagaa gatggccgat
      661 cgcggcctcg acgctgtcgt gatctacggc gacaaggagc acttcgcgaa cacgtcctac
      721 ctgagcgggt tcgatccgcg ctgggaggaa tcgctgctcg tgttcgcgcc gggtcgtacc
      781 ccgaccctgg tcgtcggcaa cgagaacgtg gcctgggcgc gagtggtccc ttacgacatc
      841 aacgtcgtgc gcgtcgcgaa gttcagcttg atcggacagc cggatcccgg cgacgtccag
      901 ctggccgacg tcctgcgtga tgcgggtatc accagggggc atgtgggtct catcggctgg
      961 aagttctgga ccggctcggc cgagcactgg atcgacgtcc ctcacttcat cgtgaccgaa
     1021 gtggccaagc tcgccggcac ggtgcagaac gcgaccgaca tcctcatgaa ccccgacggg
     1081 gggttgcgca tcgacaacga cgtcgaccag atcgccgcgt tcgagttcgg cgcgagccac
     1141 gcctcggccg cggtgaaccg gctgctgcac ggcatcgcgc ccggcatgac cgaactcgaa
     1201 gcgacccaac tgatgaatcc gattctgctg ccgttcagct accacccgac catgctcagc
     1261 ggtccgaacg cggtctacgg agtcgccagc gcgggcgatc gggtgctcga gatcggcgac
     1321 ccggtgtgcg ccggcttggg atattggggt ggcaacaccg cccgcgccgg cttcctggtg
     1381 cacgacgcct cgcagctacc cgccgccgcg agcgactacc tcgagaagat ggccgcgccc
     1441 tactacgcga ccgccgccgc ctggtacgaa acgatccgca tcggactcac cgcgggcgag
     1501 ctctacgacg tcaccttcag ccgcatcggc gacccgttct tcggcgtcta cctcaacccg
     1561 ggccacttca tccacctcga cgagtggccc tcgtcaccgg tcgccgcggg ctcgccggtc
     1621 gtcttcaagt ccggcaacgc gattcagctc gacatcatcc cgatcaccgg cagcggctat
     1681 cacacagccc aaatagagga cggcgtggtg ctggccgact cctccctgcg cgcgtcgatc
     1741 gccgccaaat accccgacgt gtgggcccgc atgcagcagc gccggaccat gttgtcgaaa
     1801 accttcggca tcgagttgca cgaagaggta ctgccgctgt cgaacacggc cggctacttc
     1861 ccgccgttct ggctggcgcc gcccctcgcg atggtccgca cctgacggtt ccgccccgac
     1921 ggagcgccgc gcgaccccga tccgccactg gctggtcggg tatttgaccg gcgcaaacgg
     1981 gtgcatggtg ttgggtctgc tggccgatgg gccgcgacca ccacgctgcc gatcacatct
     2041 tcatcaggtt tgttgccggg ggacaccacg gcgcctgact gttgtgggga ggttgatatg
     2101 tcgcgttcgc gtttgttg
//
LOCUS       DASWVF010000403         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_469327, whole genome shotgun sequence.
ACCESSION   DASWVF010000403 DASWVF010000000
VERSION     DASWVF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469327"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGD55_07270"
     CDS             complement(<1..597)
                     /locus_tag="VGD55_07270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0870182.1"
                     /translation="MARTRFAADRGLSARMVFVMFLLGLVYVLFVGVLIAIHLSPILI
                     IVIAGGFFLFQFFGSDRIALYAMHGKIVTPEEAPELHAIIDRLCAMSNTPKPKVAIAD
                     SDVPNAFATGRSQKRAVVCATTGIMRRLDNEELEGVLAHEMSHVAHHDVVVMTIASFL
                     GVLAGLVTRFAFYATMFGGGNNRGRNNNQDSAAVVELVV"
     gene            complement(612..890)
                     /locus_tag="VGD55_07275"
     CDS             complement(612..890)
                     /locus_tag="VGD55_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870183.1"
                     /translation="MILRILGEGQFEVADHTVEALNGMDNELQAAIDAGDEAAFHTAL
                     AGLLALVREVGQPVAADHLGPSDLVLPAAESTLEEVRALLGNEGLIPG"
     gene            complement(887..1678)
                     /locus_tag="VGD55_07280"
     CDS             complement(887..1678)
                     /locus_tag="VGD55_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY0870184.1"
                     /translation="MGVMKRMSLIFNAKANKVLDRAEDPRETLDYSYTKQLELLQKVR
                     RGVADVATSRKRLELQATQLQQQADKYDGQARAALAQGREDLAREALTRKSALTQQIA
                     DLQTQHAQLLAEEEKLTQASQRLQAKVDAFRTKKETIKATYTAAEAQTKIGEAFSGIS
                     EEMGDVGLAIQRAEDKTQSMQARAGAIDELVASGALNDPTGSPSDDIQLELDRMSSTS
                     DVEAQLAALKGEIAAPAAPAAIEGAPAATTPAAAAPAEAKPEESA"
     gene            1816..2439
                     /locus_tag="VGD55_07285"
     CDS             1816..2439
                     /locus_tag="VGD55_07285"
                     /inference="COORDINATES: protein motif:HMM:NF022686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEY0870185.1"
                     /translation="MFGRGTKDAAGVSNPGDPLGAGPSAPTTKGKATPKRSEAQAARK
                     TAAIGQSSVRGGRTPSGGGSSARSGRSGRLTQQQARARRDAMRRGDESALTARDRGPA
                     RRFARDYVDARRNVIGLFVPVAVPVIILGYSKASHLQIAANIVLYTFVLGSVIDCVRT
                     SRAIRNQVTARFPNESTKGLGLYAAMRAMQFRRMRVPGPRVARGAKV"
     gene            2441..3442
                     /locus_tag="VGD55_07290"
     CDS             2441..3442
                     /locus_tag="VGD55_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEY0870186.1"
                     /translation="MKFRHLGKSGLIVSEIAYGNWLTHGSQVEADAATACVRAAFDVG
                     ITFFDTADVYAQTRAEAVLGDALAGVRRESYELATKVFWPTGPGQNDRGLSRKHIIES
                     CHASLKRLRTDHLDLYQAHRFDVETPLEETLRAFDDLVRQGKVHYIGVSEWSAPQIAD
                     ALRIAADMGLDAIVSNQPQYNMLWRVIEAEVVPLCERAGIGQIVFSPLAPGVLTGKYQ
                     PGAAPPAGSRATDTHGGAGMISRWMGDDLLSRVQLLQPLADDAGLSMATMALAWVLQN
                     PNVASAIIGASRPEQVHENVKASGITLDADLLARIDEVLDPIVERDPAKSQQLAHRP"
     gene            complement(3534..3704)
                     /locus_tag="VGD55_07295"
     CDS             complement(3534..3704)
                     /locus_tag="VGD55_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870187.1"
                     /translation="MTPVDLPPAESFPTQSDAESWLGEEWRPLLAAGVSRVSLLEGDR
                     VVYGPMSLDPES"
BASE COUNT          682 a         1286 c         1270 g          663 t
ORIGIN      
        1 aacgacgagt tcgacaactg ccgcactgtc ttggttgttg ttgcgcccgc ggttgttgcc
       61 gccgccgaac atcgttgcgt agaaggcgaa tcgggtgaca agaccggcta gcacgccgag
      121 gaacgaggcg atcgtcatca ccacgacgtc gtggtgagca acgtgcgaca tctcgtgcgc
      181 gaggacacct tcgagctcct cattatcgag ccgccgcatg atgccagtgg tcgcacacac
      241 cactgctcgt ttctggctgc gcccggtcgc gaacgcattg ggcacatcgc tgtcggctat
      301 cgcgaccttg ggcttcggag tgttcgacat cgcgcagagc cggtcgatga tggcgtggag
      361 ttcgggtgct tcttcaggcg tgacgatctt gccgtgcatc gcgtacaacg cgatgcggtc
      421 ggaaccgaag aactggaaca ggaagaagcc gcccgcgatc acgatgatca ggatcggcga
      481 caggtggata gcgatgagca cgccgacgaa caggacgtac acaagaccga gcaagaacat
      541 gacaaagacc atgcgagccg acagaccgcg gtctgcggca aaacgagttc gggccataag
      601 acggacgccg gtcagccagg gatgagtccc tcattgccga gcagcgcacg cacctcctcc
      661 agagttgatt cagcggcagg cagcaccagg tcggacggcc cgagatgatc ggcagcgacc
      721 ggctgaccca cctcgcggac cagggcgagc agcccggcca gcgccgtgtg gaacgcggct
      781 tcgtcgccgg cgtcaatcgc cgcctggagc tcgttgtcca tgccgttaag tgcctcgacc
      841 gtgtgatcgg cgacctcgaa ctgcccctcg ccgagaatgc gcaggatcat gccgactcct
      901 ccggcttggc ctcagcaggc gctgcagcgg ctggcgttgt ggctgccggt gccccttcga
      961 tggcggcagg cgctgccggg gccgcgatct cgcccttcaa cgccgcgagc tgcgcctcga
     1021 cgtcgctcgt gctcgacatg cgatcgagtt cgagctggat gtcgtcgctc ggcgaacccg
     1081 tcgggtcgtt gagcgcgccg gacgcgacca actcgtcgat cgcacccgcg cgtgcctgca
     1141 tgctttgcgt cttgtcttcg gcccgctgga tcgcgagacc gacgtcaccc atctcttctg
     1201 agatgccgga gaacgcctcg ccgatcttgg tctgcgcctc ggccgcggtg taggtcgcct
     1261 tgatcgtttc cttcttggtg cggaaggcgt cgaccttggc ctgaagccgc tgagaagcct
     1321 gggtgagctt ctcctcttca gcgagcaact gggcatgctg ggtttgcagg tcggcaatct
     1381 gctgggtcag cgccgacttg cgggtgagcg cctctcgggc gaggtcttca cgaccctgcg
     1441 cgagcgctgc gcgtgcctgc ccgtcgtatt tgtcggcttg ctgctgaagc tgggtggcct
     1501 gcaactccag acgcttccgg gaggtcgcga catcggcaac gccgcgccgc accttctgga
     1561 gcagctcgag ctgcttggta tagctgtagt cgagcgtctc gcgcggatcc tcggcgcggt
     1621 caaggacctt gttggccttg gcattgaaga tgagcgacat gcgcttcatc acacccattg
     1681 gtgccgactc cttcgcttga cggtctccac ggacggctcg catgtccgta tccaccttat
     1741 gtgtcgacgg accgcaccgg cagagggttc ccggcgggcc agcgtgctca gctgccctgc
     1801 cgctaacctg tgcacgtgtt cggacgtgga acgaaggatg ccgccggggt aagcaacccg
     1861 ggcgatccgc taggtgctgg ccctagcgcc ccaacgacta aaggcaaagc aacccccaaa
     1921 cgaagcgagg cacaggcagc ccgcaagaca gcggcaatcg gtcaatccag tgttcgcggt
     1981 gggcggacgc cgagtggtgg cggcagttcc gcgcgctcgg gtcggtcggg gcggctgacc
     2041 caacagcagg cccgggcccg ccgcgacgca atgcgccggg gcgacgaaag cgcgttgaca
     2101 gcacgcgacc gtggaccggc ccgtcgcttc gcccgcgact acgtcgatgc ccgccgcaac
     2161 gtcatcggcc tcttcgtgcc agtggcggtg cccgtcatca tccttggcta cagcaaggcg
     2221 tcgcaccttc agatcgccgc caacatcgtg ctgtacacgt tcgtattggg ctcggtgatc
     2281 gactgtgtgc gcacctcgcg cgcgatccgc aaccaggtga cggcgcgctt ccccaacgag
     2341 tcgacaaagg gtctcggctt gtacgcggca atgcgggcga tgcagttccg ccgcatgcgg
     2401 gtgccggggc cgcgagtcgc acgtggcgcc aaggtctgac gtgaagttca ggcacctcgg
     2461 caagagcggg ctcatcgtca gcgagatcgc gtacggcaac tggctgacgc acggatcgca
     2521 agtcgaagcc gacgcagcga cggcatgcgt acgcgccgca ttcgatgtcg gcatcacctt
     2581 tttcgacacc gccgacgtct acgctcaaac gcgcgcggaa gcagtgctcg gagacgcact
     2641 cgcgggcgtg cgccgcgagt cgtacgagct cgccacgaag gtcttctggc ccaccggccc
     2701 gggccaaaac gaccgcggac tttcccgcaa gcacatcatc gagagttgcc acgcctcgtt
     2761 gaagcggctg cgaacagatc acctcgacct gtaccaagcg caccgcttcg acgtcgaaac
     2821 tccgctcgaa gagacgctgc gtgcattcga cgatctggtt cgccagggca aggtgcacta
     2881 catcggcgtg agcgagtgga gcgcgccgca gatcgccgac gcgcttcgca tcgccgccga
     2941 catgggcctc gacgcgattg tgtcgaacca accgcagtac aacatgttgt ggcgggtgat
     3001 cgaggccgag gtggtgccac tgtgcgagcg cgcgggcatc ggccagatcg tgttttcgcc
     3061 gctggcgcca ggggttctga ccggcaagta ccagcccgga gccgcgcctc ctgctggctc
     3121 acgggccacc gacacccacg gcggcgccgg gatgatctcg cggtggatgg gcgacgacct
     3181 gctctcgcga gtgcagctcc tccaaccgct cgccgacgac gccggcttgt ctatggcgac
     3241 gatggcgctg gcgtgggtgt tgcagaaccc caacgtggcg agcgcaatca tcggagcctc
     3301 gcgtcccgag caggtgcacg agaacgtaaa agcgtcaggc atcaccctgg acgccgattt
     3361 gctcgcgcgc atcgatgagg tgctcgatcc catcgtcgag cgcgacccgg ccaagtcaca
     3421 gcagctcgcg caccgcccct agcccatcaa cgtcgcatca acgtggagtg ctgtgagtgc
     3481 tagaacccgc tgcaaactcc acgatcccgg gcaagggtgg tcgagtcgag gggtcaggat
     3541 tcggggtcga ggctcatcgg tccgtacacg acgcgatcgc cctcaagcag gctgacccgc
     3601 gacacgccgg ccgcaagtag cggtcgccat tcctcaccga gccacgactc ggcgtccgac
     3661 tgggtgggaa aggactctgc cggaggcagg tcgacgggtg tcacgggttc accgtcggcg
     3721 ctctcgtacc gccacaacca acccaccgag cgctcccttc ggctacacct tcgatcacgg
     3781 cttcgatccg gacgccgagg cgaggctagc gcttcgacgc gagtgctcgc gctctttgac
     3841 tcgggcgcgc gcggcgtgac aagctcgcgc cacaactaaa gacgcgtccg cgttcagggg
     3901 a
//
LOCUS       DASWVF010000404         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_393029, whole genome shotgun sequence.
ACCESSION   DASWVF010000404 DASWVF010000000
VERSION     DASWVF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393029"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..385)
                     /locus_tag="VGD55_07300"
     CDS             complement(155..385)
                     /locus_tag="VGD55_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870188.1"
                     /translation="MEKRFLLLSDVAEVLNISAAQAYALVRSGELPAIKVGGRGQWRV
                     EVPVLESYIQDAYTRTRDFVSSHPFGSGEPDE"
     gene            607..1290
                     /locus_tag="VGD55_07305"
     CDS             607..1290
                     /locus_tag="VGD55_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870189.1"
                     /translation="MKSPSWLDGRLVIGVVLVLLSVGVGAKIISSSQRYDVMWAASRD
                     IAPGTTLIKSDLVQVDVRFKDHGALYISAAGPSPVGRVTVSPLANGQLIPLVAVPSTP
                     PPAVRLVTIPVERLHMPRGNDLHGVQVDLYVTPKTLNGQAQAKPQLVLANVTIADTIV
                     DSSLASSDGSGVVLSVPVAFVDAVVAAAQLGSVDLVRVPAPATAGVTVGPSSSASPSP
                     SDSVGLASP"
     gene            1287..>2111
                     /locus_tag="VGD55_07310"
     CDS             1287..>2111
                     /locus_tag="VGD55_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870190.1"
                     /translation="MSVPVLTAVTDAVWEADLVSAFEHQDFGVVVVRRCVDLADLLAA
                     AAAGTARAVILSADLRRLDRDALARLATAGVAVIGLVAQGGKPGTSDDAERRLRQLGV
                     RHVLAADAGPAVISAEVTTAVADGVATGGLDPLRLGYGDARAALPDVGALTLVPDEVE
                     ARRDDGRLVAVWGPTGAPGRTTTAVVLATEAARLGCASLLADADCYGGVIAQVLGLLD
                     ESPGLAAAVRQANAGNLDLHALATLARVVGANLRVLTGIARAERWPELRPSGLVAAY"
BASE COUNT          321 a          685 c          689 g          416 t
ORIGIN      
        1 gtaatcgtca cggtggcacc tgcttcggcc tgtcggacca gttcgctggc ttgttgacgg
       61 agctcacgca acccggtcgt cgtcatgcca accatggtag cgcatgtgct acaggtccgc
      121 gaaggctgtt cttgagacgc actacctcgc aggtctattc gtcgggctcg ccgctgccga
      181 agggatggct gctgacgaag tcgcgggtgc gtgtgtacgc gtcttggata tagctctcga
      241 ggaccggcac ctcgactcgc cattggccgc ggccgccgac tttgatcgcc gggagttcgc
      301 ccgaccgcac cagtgcgtac gcctgggccg cggagatgtt cagcacttca gcgacatctg
      361 acaagagcag aaatcgcttc tccatcgacg ctcctcaaac tcgggcagac gctgctatct
      421 acgccggaag gcgtcagtat gccatcggtt acccatgaat gctttcgtta tccacacaac
      481 acttctgttt gtcatcgcaa agtcggacga cggggcagac tgaggacagc gaggggctgc
      541 tgttccgctc tcgccaccag ttacggggga aatgtgtcgg agctacctgc gccgtcggct
      601 acacgcttga aatcgccgtc ctggctcgac ggccgcctgg tcatcggggt cgtcctcgtg
      661 ctgctctccg tgggtgtcgg cgcgaagatc atctcgtcgt cgcagcgcta cgacgtgatg
      721 tgggcagcgt ccagggacat cgcgccaggc acgacgttga tcaaatccga cttggtccag
      781 gtcgacgtgc gcttcaagga ccacggcgct ctgtacatct ccgccgcggg tcccagcccg
      841 gtcggtcgag tcacggtctc gccgctcgcc aatggccagc tgattccact ggtagcagtt
      901 ccgtcaacgc cgccgccggc cgtgcggctc gtcacgatcc ccgtcgagcg actgcacatg
      961 cccaggggca acgacttgca tggtgtgcag gttgacctct acgtcacgcc gaagacgttg
     1021 aacggccagg cgcaagcgaa gcctcagctc gttcttgcga acgtcactat tgccgacacc
     1081 attgtcgatt cgtccctcgc gagcagcgat ggttccggcg tggtcttgtc ggtgcccgtc
     1141 gcttttgtcg atgcagtggt tgccgccgcg cagcttgggt cggtcgacct ggtacgggtg
     1201 cccgctccag cgaccgcggg cgtcacagtc ggtccgtcct cgagcgccag cccctcgccg
     1261 tccgacagtg ttggtctggc ttcgccgtga gcgtccctgt tctaacggcc gtcactgacg
     1321 ccgtctggga ggccgacctg gtttcggcct tcgagcacca agacttcggc gttgtggtgg
     1381 tccggcgttg cgtcgaccta gcagacctac tcgccgcggc tgctgcagga acagcgcgcg
     1441 cggtgattct ctcggccgac ctgcgcaggc tcgatcgcga cgcattggcc cgactcgcaa
     1501 cagcaggcgt cgcggtcatc ggcctggttg ctcaaggcgg caagcccggc acgagtgacg
     1561 acgccgaacg gcgactgcgg caactcggcg tccgccatgt acttgctgcc gatgctggcc
     1621 ctgcggtgat ctccgctgag gtaacgacag cagtcgccga cggcgtcgcg accggtgggc
     1681 ttgatccgct acgtctcggc tacggcgatg ctcgcgccgc gttgcctgac gttggggcgc
     1741 tgacgcttgt gccagacgag gtcgaagccc gacgcgacga cggacggctc gtcgccgtgt
     1801 ggggtccgac aggtgcacca ggccggacga cgactgcggt cgtcctcgcg acggaggcgg
     1861 ctcgtctcgg ctgcgcgtca ttactagcgg acgccgattg ctacggcggc gtgattgctc
     1921 aagtgctggg tctgctcgac gaatcgcccg gccttgccgc ggcagtgcgt caagcgaacg
     1981 caggcaacct tgacctgcac gcacttgcga ccctcgcgcg cgtggtcggt gcgaacttgc
     2041 gagtgttgac tggcattgct cgggccgagc ggtggccgga gttgcggccg agcggactcg
     2101 ttgctgcgta t
//
LOCUS       DASWVF010000405         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_239961, whole genome shotgun sequence.
ACCESSION   DASWVF010000405 DASWVF010000000
VERSION     DASWVF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239961"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2037)
                     /locus_tag="VGD55_07315"
     CDS             complement(<1..2037)
                     /locus_tag="VGD55_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0870191.1"
                     /translation="MRLRRSSLVIGSAAAILAATAAAAAVGVVIVATSHHSTSSHPAV
                     QTVAGSVPSLTAGRGAPVPFVEQEAVNAATNGVVLAKSRAWDTLAGEAVGRTAVTLTG
                     RGKYVEFTTTQPANSLDLRYSIPDRADGSPYQATLSVYVNGHKMHDLTLTNRFSWYYG
                     AYPFTNTPNSGNPHHFYDEVHELLGPELAAGSKVRLEIDAEDTAASYTIDLADFEDVA
                     PARREPAGALSVVTFGADASGASDSSKAFTSAIAAAAAQGASVWVPAATFVVTKHLVV
                     PSNTTIVGAGPWYSVLTGAGVGIYGNTAPNPSTNVHLSDFAIEGTIDVRDDAAQVNGI
                     GGAMGGGSTISNLWIEHTKVGIWFDGPFDAVTIDHTRIDDTTADGINLHDGITNATVT
                     NDFIRNTGDDGIAAWSENDADTDDVISFDTVEVPILANNIAIYGGADNQVTDDVVSDT
                     QTQGGGIHLANRFNAVAISGTTTVARDTALRTGVLDPNWHYGVGAIWFWASDSPMAGA
                     IRVSDVDLIDSSYEGIQLTGSSVTNVAFDKVAITGAGTFALQLEAHGSATFKDVVATK
                     VGASAGTYNCLYTGSSEQAFTVTNDGGNAGWNTTFCGPWPAPVRSYEYPPEAGPDTFG
                     ISLEQPSGWAAPGGSATTTISTSVATGSTQRLQLTTGELPAGVTATFDAGSLSAG"
     gene            complement(2131..3177)
                     /locus_tag="VGD55_07320"
     CDS             complement(2131..3177)
                     /locus_tag="VGD55_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0870192.1"
                     /translation="MAKRLADVAQKVGVSEATVSRVLNGKPGVSTVTRDAVLTALDIL
                     GYERPTLLRGDRARLVGLVLPELQNPIFPALAEVVGGGLAQRGFTPVLCTRTAGGLSE
                     AEYVRMLLEQQVSGVVFAGGAYAEANASHAHFMRLHERGVPVVLVNAAVEHLGFPAVS
                     TDDAVAVEQAFGHLLSLGHERIGLVLGPADHMPSRRKLAAFTAVAAAAGIETGDAVER
                     AMFSIEGGQAAGARLLQRGLTGVICASDPIALGVIRAIRRARLDVPGDVSVVGFDDSA
                     FMNCTDPPLTTVRQPIDAMGRAVVTLLADQIDGASASEEELLFEPELVVRNSTGTARK
                     AAVRSATRGAAPAS"
     gene            complement(3234..5186)
                     /locus_tag="VGD55_07325"
     CDS             complement(3234..5186)
                     /locus_tag="VGD55_07325"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY0870193.1"
                     /translation="MEGDVLWTPSAQRVAAARLTALRDLLADRYGVDLADFSTLQRFA
                     LDHPDDFWSALWDFTGVVGDKGARAFVDAPDMRAARFFPDAAINVAETMLARTDDVPA
                     LLFAREDGKRSVTTWAQLRREVAAVAYALLDAGVEPGDVVAAWLPNVPEAYVTALAAA
                     TIGALYTSTSPDFGSSGVLDRFGQVSPKVLVATDGYLYGGRGHDCLARLKEIREGLPA
                     TRVVVVVPGLADVPDLSGIDGAVPWSDFARPTDRLPPFRRGPFDTPLLVLYSSGTTGP
                     PKCIVHRAGGILLTLLKEHQLHCDVRRDDRVFYYTTTGWMMWNWLAGALASQATLVVY
                     DGSSVYPSPMSLFELIDEFGITLFGTSARFLDTARKAGVAAISEFSSLRTITSTGSPL
                     SVGAFEYVYRDLASDVHLASISGGTDICGCFVMGDPTRPVYSGEIQGPALGMAVEIFD
                     ERGVPAPAGVTGELVCTRPFPSMPLGLWADADGSAYNAAYFAAYPGVWRHGDWASRTA
                     HDGFVISGRSDATLNPGGVRIGTAEIYRQVDQLPQVLESVVVGQRRGDGDDDERVVLF
                     VHLVDGVELDDELAATIRSVIRRGCSPRHVPEVIVSVPDIPRTRSGKLVELAVRSVVD
                     GRAVSSVDALANPESLEFFRNRPELR"
BASE COUNT          958 a         1782 c         1750 g          872 t
ORIGIN      
        1 gcccgcagac aacgagccgg cgtcaaacgt cgcggtcaca cccgcgggca gctcacccgt
       61 tgtgagctgg aggcgctggg tcgagccggt ggctaccgac gtgctgatcg tggtcgtggc
      121 agatccgccc ggcgcggccc atccggaagg ctgctcaagg ctgatgccga aggtgtcggg
      181 gccagcctcg ggcgggtact catagctgcg taccggcgcc ggccatggcc cgcaaaacgt
      241 ggtgttccag cccgcgttcc caccatcgtt ggtgacggtg aaagcctgct cagagctacc
      301 ggtataaagg cagttgtacg tccccgcgga agccccgacc tttgtcgcga ctacatcctt
      361 gaaggtcgcc gatccgtggg cttcgagctg aagcgcgaaa gttccggctc cggtgatcgc
      421 gaccttgtcg aaggcgacgt tggtgaccga tgacccggtg agctgaatgc cctcgtagga
      481 gctgtcgatc agatcgacgt cgcttacccg aatcgcccca gccatcggcg aatcgcttgc
      541 ccagaaccag attgcgccca caccgtagtg ccagttcggg tcgagtaccc cagtgcgaag
      601 cgcagtgtct cgcgcgactg tcgtggtgcc agaaatcgcc accgcgttga acctgttggc
      661 aaggtggatc ccgccgccct gcgtctgcgt atccgacacc acgtcgtcgg tcacctggtt
      721 gtcggcgccg ccgtagatcg cgatgttgtt cgccaatatc ggcacctcga cggtgtcgaa
      781 actgatcacg tcgtcggtgt cagcgtcgtt ctccgaccat gccgcgatcc cgtcgtcgcc
      841 ggtgttccgg atgaaatcgt tcgtcaccgt cgcgttggtg atgccgtcgt gcaggttgat
      901 gccgtcggcc gtcgtgtcgt cgatgcgggt gtggtcgatc gtgacggcgt cgaacggccc
      961 gtcgaaccag ataccgacct tggtgtgctc gatccacagg ttcgagatcg tcgagccgcc
     1021 acccatcgcg ccaccgatgc cgttgacttg cgcagcatcg tcgcggacgt cgatcgtgcc
     1081 ctcaatcgcg aaatccgaca gatgcacatt cgtgctcggg ttgggcgccg tattgccgta
     1141 gataccgact ccggcgccgg tcagcacgct gtaccaaggc ccggctccga cgatcgtcgt
     1201 attactcggg acgacgaggt gtttcgtcac tacgaacgtg gccgccggca cccataccga
     1261 agcgccctgc gcagccgcag cggcgatcgc cgacgtaaac gccttcgagg agtcactggc
     1321 gcccgaggca tcagcgccga aggtgactac cgaaagggcg ccagccggtt cgcgccgcgc
     1381 aggcgcaacg tcttcgaagt ctgcgaggtc gatcgtgtag gaggccgcag tgtcctccgc
     1441 gtcaatctcg agccgcacct tcgaccccgc tgcaagttct gggccgagca gctcgtgcac
     1501 ctcgtcgtag aagtgatgcg gatttccgct gttcggcgta ttcgtgaacg ggtaggcgcc
     1561 gtagtaccag ctgaatctgt tggtcaacgt caggtcatgc atcttgtggc cgttgacgta
     1621 cacagaaagc gtggcctggt agggcgaacc gtcagcacgg tcgggaatgc tgtagcgcag
     1681 gtcgagtgag ttcgccggct gtgtagtggt gaactcgacg tacttgccgc gacccgtgag
     1741 cgtgaccgcg gtgcgtccca ccgcctcacc ggccagcgtg tcccaggcgc ggctcttagc
     1801 cagcaccacg ccattggttg ccgcgttcac ggcttcctgc tcgacgaacg ggactggcgc
     1861 gccgcggccc gccgtcagtg atggaactga gcccgcaacg gtctgtaccg ccgggtgtga
     1921 cgaggtcgag tgatggctcg tagcgacgat gacaacgccg accgcggcag ctgcggcagt
     1981 cgcagccagg atcgcggccg ccgaaccgat caccaggctg gaccggcgca gtcgcatgcg
     2041 ggcacgatgg cacgctatgc gcgtacgttg caagagattg acgaaactac gaacgaactt
     2101 gacaaccgac tatccctgcc ggcccgtctg ctaactggct ggcgctgccc cgcgggtagc
     2161 cgagcgaacg gccgctttgc gggcggtgcc cgtgctgttg cgaaccacta gctccggctc
     2221 gaacagcagc tcttcctccg acgcgctcgc gccgtcgatc tggtcggcga gcagggtcac
     2281 caccgctcgg cccatggcgt cgatgggttg acgcaccgtt gtcaggggcg gatcggtgca
     2341 gttcatgaaa gccgagtcgt cgaatccgac aacggagaca tcaccgggaa cgtcaagtcg
     2401 ggcgcggcgg atcgcccgga tcaccccgag tgcgatcgga tcgctcgcgc agatgacgcc
     2461 ggtaagcccg cgctggagca accgagcacc cgccgcctga ccgccctcga tcgagaacat
     2521 cgcgcgctcg accgcatcac ccgtctcgat gcccgccgcg gcagccacgg cggtgaatgc
     2581 ggcgagtttg cgccgagagg gcatgtggtc ggcggggccg agcacgaggc cgattcgctc
     2641 gtgcccaagt gagagcaggt ggccgaacgc ttgctcgacc gcgaccgcgt catcggtcga
     2701 cacagccggg aacccgaggt gctcgacggc tgcgttcacc agcacaacgg gaaccccccg
     2761 ctcgtgcaag cgcatgaaat gagcatgcga tgcgtttgcc tccgcgtagg caccgccggc
     2821 gaagacgaca cccgacacct gctgctcgag gagcatccgg acgtactcgg cttcggacag
     2881 cccacccgcg gtgcgggtgc acagcaccgg ggtgaacccg cgctgggcaa gaccgccacc
     2941 cacgacctcg gcgagcgccg ggaagatcgg gttctgaagc tccggcagga ccaggcccac
     3001 cagccgcgcg cggtcgccgc gtaggagcgt cgggcgctca tacccgagga tgtcgagcgc
     3061 ggtcaggacc gcgtctcggg tcactgtcga gactcccggc ttgccgttta gcacccggct
     3121 gacggtcgcc tcgctgacgc caaccttctg cgcaacatct gccagtcgtt tagccaccag
     3181 ccaaagttag cgcaagtcac gcaagttccg cgcaagtacc gcaagctctg acgtcagcgc
     3241 agctcagggc ggttgcggaa gaattcgagg ctttcagggt tcgccagggc atcgaccgag
     3301 ctcacggcgc ggccatcgac gacgctgcgg accgcaagct cgacaagctt tccgctgcgc
     3361 gtgcggggga tatcggggac gctcacgatc acctccggca cgtgacgcgg cgagcacccc
     3421 cgtcggatca ccgacctgat cgtggccgca agctcgtcgt cgagctccac gccgtcgacc
     3481 aggtgcacga agagcacgac ccgttcatcg tcgtcaccgt cgcctcgccg ctgcccgaca
     3541 acgacacttt caagaacctg cggcagctgg tcgacctggc gatagatctc agcggtcccg
     3601 atccttactc cacctggatt cagcgtcgca tcggatctgc cggagatcac gaagccgtcg
     3661 tgcgcggtgc gcgaagccca gtcaccatgg cgccaaacgc cggggtaggc ggcgaaatac
     3721 gccgcgttgt aggccgaccc gtcggcgtcc gcccaaagcc ctaacggcat cgaagggaac
     3781 gggcgggtac agacgagttc gcccgtgaca cccgctggtg caggcacgcc gcgctcgtcg
     3841 aagatttcga cggccatgcc gagggccggg ccttgaattt ccccgctgta gaccggacga
     3901 gtcggatcgc ccatcacgaa gcatccgcag atgtcggtac ccccggagat cgacgcaaga
     3961 tggacgtcgg aagcgaggtc gcggtacacg tactcgaatg ctccgacgct gagcggcgag
     4021 ccggtcgacg tgatcgtgcg caggcttgag aactcgctga tcgccgccac gcccgccttg
     4081 cgtgccgtgt cgagaaacct ggccgaagta ccgaatagcg tgatgccgaa ctcgtcgatg
     4141 agctcgaaga gtgacatggg cgatggatag accgacgagc cgtcatagac gacgagcgtc
     4201 gcctgcgagg cgagcgcgcc ggctagccag ttccacatca tccagccggt cgtcgtgtag
     4261 tagaagacgc ggtcgtctcg cctgacgtca caatgcagct ggtgttcttt caagagcgtg
     4321 agcaagatgc cgccggcccg gtgcacgatg cacttcggtg gtccggtcgt gccagacgag
     4381 tacaagacca gcaggggtgt atcgaagggc ccccgccgaa acggcggcag ccgatcggtc
     4441 gggcgcgcga agtcgctcca cggcacggcg ccgtcgatcc ccgacaggtc aggcacgtcg
     4501 gcaagacctg gcacgaccac cacgacgcgc gtagccggca ggccttcgcg aatctccttc
     4561 aggcgcgcga gacagtcgtg gccgcggccg ccgtagaggt agccgtcggt cgccacaagc
     4621 accttcgggc tcacctgccc gaagcggtcg agcacgccgc tgctgccgaa gtcgggggac
     4681 gtcgaggtgt acagcgcgcc gatcgtcgcg gcggcgagcg cggtgacgta agcctccgga
     4741 acgttcggca gccacgccgc gacgacgtcg ccaggctcga cgccggcatc gagcaacgca
     4801 tacgcgactg cggctacctc gcgccgtagc tgcgcccacg tcgtgacaga tcgcttgccg
     4861 tcttctcgag caaagagaag ggccgggacg tcgtcagtgc gcgcgagcat ggtctcggcg
     4921 acgttgattg ccgcgtcggg gaagaaccgc gcagctcgca tgtcgggcgc gtcgacgaag
     4981 gcccgagcgc ccttgtcgcc cacgacaccc gtgaagtccc aaagggccga ccagaagtca
     5041 tcagggtggt cgagggcgaa ccgttgcagc gtcgagaaat ccgccagatc gacgccgtac
     5101 cggtcagcga gcaagtcgcg caacgccgtc aagcgagcgg ccgctacccg ctgcgccgac
     5161 ggcgtccaca ggacgtcacc ctcgatgcgc catcacctct cgaaccggcc gctgatcgag
     5221 ctcacgacgt gctcgggccg gcccacaagt tgacgccgcc ctcgaccgcg tagtggtcaa
     5281 tttcggccag ttcatcggaa ctgaattcga gattctcgag tgctccgacg ttttgatcga
     5341 gctgggcgac gctgctggcc cc
//
LOCUS       DASWVF010000406         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163394, whole genome shotgun sequence.
ACCESSION   DASWVF010000406 DASWVF010000000
VERSION     DASWVF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163394"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGD55_07330"
     CDS             complement(<1..736)
                     /locus_tag="VGD55_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0870194.1"
                     /translation="MLASVPALKLDPDVEMLRFATAGSVDDGKSTLVGRLLHDSKSVL
                     TDTLEAVERASRDRGSDGVELALLTDGLRAEREQGITIDVAYRYFTTARRKFVLADTP
                     GHVQYTRNMVTGASTAELVVILIDARKGIVEQTRRHLAVAALLGVRHLVLAVNKMDLV
                     DFDEAVFTKIADEFAAAAEALGAGGHVVIPVSAHLGDNVVERSTSMPWYTGPTLLEHL
                     EAVPVGFDAWIQPARLPIQYVVRATDY"
     gene            complement(764..1696)
                     /gene="cysD"
                     /locus_tag="VGD55_07335"
     CDS             complement(764..1696)
                     /gene="cysD"
                     /locus_tag="VGD55_07335"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0870195.1"
                     /translation="MSVATAVPSSSVQGRLPHLDVLESEGIHIIREVAAEFERPVLLF
                     SGGKDSVVMLHLARKAFWPAPVPFPLMHVDTGHNFPEVMTYRDDVVERFGLRLLVMSV
                     QDYIDDGRLRERSDGTRNPLQTVPLLDGIVSNRFDAVFGGGRRDEEKSRAKERIFSLR
                     DEFGQWDPRRQRPELWNLYNGRHKAGEHVRVFPLSNWTELDVWQYVQRESIELPSIYL
                     AHQRDVFLRGGMWLTAGEWGGPKPGEPTERKVVRYRTIGDMSCTGAVESDASDVAEVI
                     AEVALSTITERGATRADDKASEAAMEDRKREGYF"
BASE COUNT          314 a          603 c          592 g          309 t
ORIGIN      
        1 gataatcggt cgccctgacg acgtactgaa tcggcagtcg ggccggctgg atccacgcgt
       61 cgaagccgac gggcaccgcc tcgaggtgct cgagcagggt cgggccggtg taccagggca
      121 tcgaggtcga acgctcgaca acgttgtcgc ccagatgcgc cgacaccggg atcaccacgt
      181 gcccgcctgc acctaacgct tcggccgcgg cagcgaactc gtcggcaatc ttggtgaaca
      241 ccgcttcgtc gaagtcgacg agatccatct tgttcacagc aagaacgaga tgccggacgc
      301 cgaggagtgc cgccactgct aaatgccggc gcgtctgttc gacaatgcct ttgcgcgcgt
      361 cgatcaagat gacgacgagt tcggcggtcg aggcgcccgt caccatgttc cgcgtgtact
      421 gcacgtgacc cggggtgtcg gcgagcacga acttgcgccg agcggtcgtg aagtagcggt
      481 aggccacgtc gatcgtgatg ccctgttcgc gctcggcgcg caatccgtcg gtcagcagtg
      541 cgagttcgac gccgtcgctg ccgcggtcgc gactggcccg ctcgaccgct tccaacgtgt
      601 cggtgagcac cgacttcgag tcatgcagca accgcccgac cagggttgat ttgccgtcgt
      661 cgaccgaacc ggccgtcgca aaccgcaaca tttcgacgtc cggatcgagc tttaacgcag
      721 gaacggaagc gagcacggag gcattctcgg atagcaacat ttcctagaag taaccttccc
      781 gcttgcgatc ctccatagcc gcctcggagg ccttgtcgtc ggcgcgggtc gcacctcgct
      841 cggtgatggt gctgagcgcg acctcagcga tgacttccgc gacgtcggac gcgtcggact
      901 cgaccgcgcc ggtgcagctc atgtcgccga tcgtgcggta gcgcacaacc ttgcgttcgg
      961 tcggctcgcc gggctttggg ccaccccact cacccgcggt cagccacatg ccgccgcgaa
     1021 ggaagacgtc gcgctgatgg gcgaggtaga tcgacgggag ctcgatcgac tcgcgctgga
     1081 cgtattgcca cacgtcgagt tcggtccagt tcgacaacgg gaagacgcgc acgtgctcgc
     1141 ccgccttgtg gcgcccgttg tagaggttcc aaagctctgg ccgctgccgg cgtggatccc
     1201 actggccgaa ctcgtcgcgc aggctgaaga tgcgctcctt ggcgcgcgac ttctcctcgt
     1261 cccggcggcc gccgccgaac accgcatcga agcggttgct cacgatgccg tcgagcaacg
     1321 ggacggtctg caacggattg cgggtgccgt cgctgcgttc gcgcagcctg ccgtcgtcga
     1381 tgtagtcctg cacgctcatg acgagcagcc gcaacccgaa ccgctcgacg acatcgtcgc
     1441 ggtaggtcat gacttcggga aagttgtgcc cggtgtcgac gtgcatcagc gggaacggca
     1501 ccggcgccgg ccagaacgct ttgcgcgcca ggtgcaacat gacgacggag tccttgccgc
     1561 cggagaacag cagcaccggg cgctcgaact cggccgcaac ctcgcggatg atgtggatgc
     1621 cctccgattc gaggacgtcc agatgaggca gccggccctg gacggacgac gacggcaccg
     1681 cggtcgcgac actcatgcca ggcctctctc ggtcagtgcc tggtacacgg cagcagctga
     1741 ttcgtcgaca gaacggccgg cggtgtcgag cctgaggtcc ggcgcggtcg gcacctcata
     1801 cgggtcgtag acccccgt
//
LOCUS       DASWVF010000407         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_393186, whole genome shotgun sequence.
ACCESSION   DASWVF010000407 DASWVF010000000
VERSION     DASWVF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393186"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGD55_07340"
     CDS             complement(<1..247)
                     /locus_tag="VGD55_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870196.1"
                     /translation="MSTLPPEPTDEHELWAELAAGHAMSSLDDADEAMYIQHSASCEV
                     CRDLERDLSLVLADLAHATHPVEPPAALKESIMAAVRE"
     gene            complement(244..858)
                     /locus_tag="VGD55_07345"
     CDS             complement(244..858)
                     /locus_tag="VGD55_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870197.1"
                     /translation="MAVDQRGSAYETYDDPSLGKLIAERDADALEELYDRHGGACYGL
                     ARRVVGDDQLAQDVVQEVFLAIWRGAATYDGSRGSLSTWLFALTHHKSVDAVRRSQRH
                     SGRRAPEEALTTEPDPAPGVDEQAMASVRRDQVRAALADLPEAQRKALMLAYFGGYSQ
                     SEIAQLTGIPLGTVKTRTLAALRRLRGVLDEAQLRTVSPEGEGA"
     gene            1173..2228
                     /locus_tag="VGD55_07350"
     CDS             1173..2228
                     /locus_tag="VGD55_07350"
                     /inference="COORDINATES: protein motif:HMM:NF038135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEY0870198.1"
                     /translation="MRWESQGIDSDVSPSASAQTLFARPAVVRTFDSPQFAGMTFFEV
                     HAKSILNKVPGASRLPFGWTINPYRGCSHACRYCFARNTHTYLDLGAGLDFDTKVVVK
                     VNAPSLLRKELGRPSWGGHPVAMGTNVDCYQRAEGRYRLMPGILTALTEAANPFSILT
                     KGSLILRDLPLLRRAAEVTDVSTAVSVGFVDPVLWREVEPGTPNPQKRLEVCRTLNAA
                     GVRCSVLMAPILPGLTDSPDQLARTVTAIATAGACSVTPIVLHLRPGAREWFMGWLDA
                     AHPELVAMYAAMYARSAYAPKPYQDDVCAQVRDLARAAGIGTAHPVDARRVPHPSDDL
                     PHAAVGTHPVGDQLSLL"
BASE COUNT          361 a          771 c          751 g          400 t
ORIGIN      
        1 cttcgcgaac ggcagccata atcgattcct tcaacgccgc cggcggctcc acgggatgtg
       61 tcgcgtgggc gaggtcggcc agcacgaggc tgaggtcgcg ttccaggtcg cgacacacct
      121 cacacgacgc cgagtgctgg atgtacatgg cttcgtcggc gtcgtcgagc gacgacatcg
      181 cgtggcctgc ggcgagctca gcccacagct cgtgttcgtc ggtcggctcg ggaggaaggg
      241 tgctcatgct ccctcccctt cggggctgac ggtgcgcagt tgagcctcgt cgaggacgcc
      301 gcgcaaccgc cgcaacgctg ccagcgtgcg ggtcttgacg gtgccgagtg ggatgccggt
      361 gagctgggcg atctccgact gcgagtaacc cccgaaatat gcgagcatca atgccttccg
      421 ctgggcctcg ggcaggtcag ccagggccgc gcgcacctgg tcacggcgca ccgaggccat
      481 cgcctgttcg tcgacacccg gcgccgggtc gggctcggtc gtcagcgctt cctcaggtgc
      541 gcggcggccg ctgtgccgct gtgatcgcct gacggcgtcg accgacttgt ggtgagtcag
      601 agcgaacagc caggtgctga gggagccgcg cgagccgtcg tacgttgcag cgccgcgcca
      661 aatcgcaaga aacacttctt ggacgacgtc ttgtgccagt tgatcgtcgc ccaccaccct
      721 gcgggctagg ccgtagcacg cgcccccgtg acggtcatag agctcctcga gcgcgtccgc
      781 gtcacgttcg gcgatcagct tgcccaggct ggggtcgtcg tacgtttcgt acgccgaacc
      841 ccgttgatcg acagccacag cgccgagtct cacatatggc gacggcacag caggagcaga
      901 cggcacatcc cccgcccggc ggacctccgg aatgcggagg attcgggtgg gtgcgaggtg
      961 ccgcgtgagc gagctgacag acaacaacct cacgctcctc acgcccgggt tgaaggggct
     1021 gacgccgttc acgagggcta gtggacggta gccacgaccg aaccggcgcg ggtaccgaac
     1081 gcggccctgg aaccatttcg ctgggtggag cgttgccggt tcaccccggg cggggtcctg
     1141 gtcactcgtt cgaacatatg ttctaatgcg ctatgcgctg ggaatcgcaa ggcatcgata
     1201 gcgacgtcag cccctcggcg tccgcccaaa cgctcttcgc acgacccgcg gtggttcgga
     1261 ccttcgactc tccgcagttc gccggcatga cgttcttcga ggtgcacgcg aagtcgattc
     1321 tgaacaaggt gcctggtgcg tcgcggctgc cattcggctg gaccatcaat ccctaccggg
     1381 gctgctcgca cgcctgccgg tactgctttg cacgcaacac gcacacctac ctcgacctgg
     1441 gcgccgggct cgacttcgac acgaaggtcg tcgtgaaggt gaatgcgccg agcctgcttc
     1501 gcaaagagct cgggagaccg tcgtggggcg ggcacccagt cgcgatgggc accaatgtcg
     1561 attgttacca gcgggccgag ggccgttacc gattgatgcc aggcatcctc accgcgctga
     1621 ccgaagcggc gaatccgttc tcgatcctca ccaaaggctc gctgatcctg cgcgacttgc
     1681 cgttgctgcg ccgcgccgcc gaggtcaccg atgtctcgac cgcggtctcc gtcgggttcg
     1741 tcgatcccgt cttgtggcgc gaggtcgagc cgggcacgcc gaacccgcag aagcgcctcg
     1801 aggtgtgccg aaccttgaac gcagctggcg tcaggtgctc ggtgctgatg gcgccgatcc
     1861 tgccgggtct caccgactcc cccgatcaac tcgcccgcac cgtgaccgca atcgctactg
     1921 ccggcgcgtg ttcggtgacg ccgatcgtgt tgcacctgcg cccaggcgcg cgcgaatggt
     1981 tcatggggtg gctcgacgct gcgcacccgg agctggttgc gatgtacgcc gcgatgtacg
     2041 cacgctcggc gtacgcgcca aagccctatc aggacgacgt gtgcgcgcag gtgcgcgacc
     2101 tcgcgcgcgc cgctggtatc ggaactgccc accccgtcga tgctcggcgc gtgccgcacc
     2161 catccgatga cttgcctcac gccgcggttg gcacacaccc ggtcggtgat caactctcgc
     2221 tgctatgacg cacgctagat tctgcggtcg tggggaaacc gctgatcgtg ctcgacatcg
     2281 atg
//
LOCUS       DASWVF010000408         7605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_494912, whole genome shotgun sequence.
ACCESSION   DASWVF010000408 DASWVF010000000
VERSION     DASWVF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494912"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGD55_07355"
     CDS             complement(<1..739)
                     /locus_tag="VGD55_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0870199.1"
                     /translation="MPDSGFDDQLAQLSREELDARSIAELDAGRLPLRAQQRLATMRD
                     DAAFTSALSVDEHHAIRSVGFSPVGQVMGTCVVHPGAYALSMCGYYGFGMQYAWGAAP
                     VVVADQVEAALRRARHSAVSRMVQECEGLRGDGIVGVRLTMQPFIAGGIEVQAIGTAV
                     RADGDVRPPVPFTSDLSGQDFAKLLLSGWLPAGLVMGTAVLIRHDDYTTAWQSGSWNN
                     TEVSGVTALVTSTRDRARHTLRADAARF"
     gene            complement(743..1165)
                     /locus_tag="VGD55_07360"
     CDS             complement(743..1165)
                     /locus_tag="VGD55_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870200.1"
                     /translation="MSTFGQGPDSGQPSLELIAAGLRRDAQDSRLHGQVVLDSLASAL
                     PPELARVERSGGLVRKAKVVGVHISVGDRRYVLKATVSGLASSVCHESGGIVMSTTPV
                     EFDAWVRELLTALSNAAGRSSSAALALQQLAISGTMQG"
     gene            1339..1755
                     /locus_tag="VGD55_07365"
     CDS             1339..1755
                     /locus_tag="VGD55_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870201.1"
                     /translation="MLLIGLLIVVAAAAFAGAVVSENWGGTTYTVHGFGHVLGHLTLA
                     EIFLSGVILTTIFFLGLWVASVSSRIRRRTSNRRRAEARATREERDALLADRDRLARD
                     LDAERAAHPIVVPEYQSQTQPAEVMEPRPATTSVMG"
     gene            complement(1798..2094)
                     /locus_tag="VGD55_07370"
     CDS             complement(1798..2094)
                     /locus_tag="VGD55_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870202.1"
                     /translation="MGGGLVQVGILLVATVIGLPCFFFTLLALLDHFEQGLTNAPRAV
                     VRRVQVVQLSAATESDAATELTGASVVELPHAVQAHDAQASEPFTVNPARAATG"
     gene            complement(2247..2816)
                     /locus_tag="VGD55_07375"
     CDS             complement(2247..2816)
                     /locus_tag="VGD55_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870203.1"
                     /translation="MCRRALLGLEPTPQAAGEARRFVASTCKRWGISNVTDELTLAVS
                     ELVTNAVLYARGHIDVELCVSDHTLVLCVGDKDIRPPVLRPVRLDLLADLDAVPSRGL
                     GSDTDERHPIMSVGASGSVAGGRGLLIVDALADEWGVSERAGGKEVWLTMPVDWPHGQ
                     PCTCAGHTRSPRPGHGCRHIDGPWDETDH"
     gene            complement(2892..3620)
                     /locus_tag="VGD55_07380"
     CDS             complement(2892..3620)
                     /locus_tag="VGD55_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870204.1"
                     /translation="MAEAPAKPAFYSRGRGDGGWRDWLSLLHPPYTAWNLSYVAIGSA
                     LAHPIRLDRLGWSLLGFFLGLGIGAHAFDELHGGPLRTGIASWALATVGGVAILTAAV
                     DGWLVGGLRLLPFIAVGAFLALGYNLEWFGGFLHNAVGLSLSWAAFPVLTGYYAQHWT
                     MSAAAVLVALGAAAQIFAQRTLSTPARWLRRSVRDVRLTATASDGTPVTLTAVDLLRP
                     LEIALKVLSWSFVPVAVGLVLAAR"
     gene            complement(3613..4353)
                     /locus_tag="VGD55_07385"
     CDS             complement(3613..4353)
                     /locus_tag="VGD55_07385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870205.1"
                     /translation="MGTDRAPTELNSFARSLFQDLPSRYDTLAYLLSFGQDRRWRAAV
                     VERVTAARPKRVLDVACGPAGVTLAIANACDAKLVGIDLTEDMLRQGLTNVRLAGAGE
                     QIKLAVARAEELPFPDGTFDAISFSYLMRYVDDPATTIAELARCLAPGGTIASLEFHV
                     PAWTPARVVWWFYTRFVLPAAGAVTGGRAWYRVGRFLGPNISYHYLRHPVGAHVANWR
                     AAGLADVRVQRMSFGGGLVMWATRADGG"
     gene            4441..5181
                     /locus_tag="VGD55_07390"
     CDS             4441..5181
                     /locus_tag="VGD55_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870206.1"
                     /translation="MTAWRDLVDQAAGQLGPVSPTLARHLRTELDEVEQRVGFIERSA
                     PRRSTRDGLHALVALTSARLADTWDPGKLSDVATAVELAYRASLHHRVVSDVNPAGLR
                     SPNTRVVLDGDWSITQAAVLVADIGPSAYRILVRGYGATQVGQLCGVGDPLGLMRAAV
                     ELGALVTEDGHGDAAQRVWRDAAQGLWRDASLSPSRRSTPGAVLAWAVAVQSAEGTKK
                     ARKVAAAGAADVHPSSRSTTTTPAVGRS"
     gene            complement(5070..5591)
                     /locus_tag="VGD55_07395"
     CDS             complement(5070..5591)
                     /locus_tag="VGD55_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0870207.1"
                     /translation="MHRLFIVRHAKAVTHPPTASGGDHERQLAPRGIAVLPGVARSIT
                     EALDGHPLDLVVCSTSARTRETVEKLAAAAPVIASARLKLESKVYLADAARLLKIVRK
                     LPASAETVLICGHNPGFHELALLLLGGDATPERLRYDLPTAGVVVVDLEEGWTSAAPA
                     AATLRAFLVPSAD"
     gene            5875..>7605
                     /locus_tag="VGD55_07400"
     CDS             5875..>7605
                     /locus_tag="VGD55_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0870208.1"
                     /translation="MRSDGSKGRQATAAPRRRRRVPLFAVYAAASLVPVAALGAVLAV
                     TYRADTNNSAMAEARSQASLLADDVVGPQLGASPLQGSIPASAGPAMAAVFSRASGEN
                     RLLRLRIRDMSGEVVWADDGSRNTAEAPGDEVLDAVHGGVVSDLTELNSDPGDTGPVG
                     PKVVEIYQPIDVGSARTEVGVLEMYLPYAPIEADVTAGLHRMYELLGAGLLALWLVLA
                     GISASTTRRLRKRAERLQYIAHHHVASGLLNAEGFSDGLQRHLHADADHEAFAPAVAL
                     IDVSRFREINEALGRARGDEVLLELGRRFAAVAGEGALVARLGGDEFAVASITDHDAD
                     LHDWALRLQAAVAEPVQADGIPVLIEVAVGYYRSEPGQPVDDLIVHADAALGRAKSSV
                     DRCAGYVDRPDSAASSVALLPRFRRAISAGELELHYQPKTKLSDGTVVAMEALVRWNL
                     DGELLPPGAFLPSIEQTAMIHPLTDWVARAALAQLAEWGPATSGLEVAINVSARNVSD
                     PGFAARLLGIVAESGVEPHRLTVEITETALFVDPDAARLCLGTLHDAGIALSIDDFGQ
                     GQTSLAYLAAL"
BASE COUNT         1228 a         2521 c         2583 g         1273 t
ORIGIN      
        1 cgaagcgagc cgcatcggca cgcaacgtgt ggcgcgcacg gtcacgcgtg cttgtcacca
       61 acgcagtgac gccgctcact tccgtgttgt tccacgagcc gctctgccat gcggttgtgt
      121 agtcgtcgtg gcggatcaat acagcggtac ccatcacgag gccggctggc aaccacccgc
      181 tcagcagcaa cttggcgaag tcttgccccg acagatccga ggtgaagggg acaggtggtc
      241 gtacatcgcc gtcggcacgc accgccgtcc cgattgcttg cacttcgatt ccaccggcaa
      301 tgaacggttg catcgtcagc cgtacgccca cgatcccgtc gccgcgcaaa ccctcgcact
      361 cctgcaccat gcggctgact gcggaatgtc gtgctcttcg cagcgctgct tcgacttggt
      421 cggcgaccac caccggtgcc gcaccccatg cgtattgcat gccgaatccg tagtagccac
      481 acatgctgag cgcgtacgcg ccgggatgga cgacgcacgt tcccatgacc tggccgaccg
      541 gtgagaagcc gaccgatctg atggcatggt gctcatcgac gcttagcgct gacgtgaacg
      601 ccgcgtcgtc acgcatcgtc gcgagccgct gttgcgcgcg caacgggagt cgccccgcgt
      661 cgagttcggc gatgcttcgc gcgtcgagtt cttcgcgact gagttgcgcg agttgatcgt
      721 cgaagcctga gtcaggcact cgttagccct gcatcgttcc gctgatcgca agttgttgaa
      781 gggccagcgc cgccgacgag ctgcggccag cggcgttcga caacgccgtc agcagttcac
      841 gtacccacgc gtcgaactcg accggcgtcg tgctcatgac gatgccgccc gactcatgac
      901 acaccgatga cgccaatccg gacaccgttg ctttcagcac gtatcgacgg tcaccgacgc
      961 tgatatggac gccgacgacc ttggccttgc ggaccaggcc tcccgagcgt tcaacccgcg
     1021 ccaattccgg cggcaacgcg ctcgcgagcg agtcgagcac cacttgcccg tgcaggcgcg
     1081 agtcctgcgc gtcgcggcgt aggccggcag cgatcagctc gaggctgggc tggccggagt
     1141 cgggtccctg gccgaaagtg ctcatcaatc tcccgccctc gcgcgcgcaa tttgtggccg
     1201 tatgcgcaga tggtccacat gctgccaccc cgttgtccac caatcatccc tggttcatca
     1261 cttcttcggg gatgggacgg cgtggggcgg ggtagggact ggctggatgt ggtcgatcag
     1321 acgaaagggt gaaacgcgat gcttctcatt ggactgttga ttgtggtcgc tgcagcggcg
     1381 ttcgccggcg ctgtggtctc cgagaactgg ggcggcacga cgtacaccgt ccacgggttt
     1441 ggtcatgtgc tcggccactt gacgctcgcg gaaatcttcc tgtcgggggt gatcctgacg
     1501 acgattttct tccttggtct ttgggtggcc agcgtgtctt cgcggattcg ccgccgcacg
     1561 tccaatcgcc ggcgggccga ggcgcgcgca actcgtgaag agcgcgacgc attgctcgcg
     1621 gaccgcgacc gcttggcaag ggatctcgat gccgagcggg ctgcgcatcc gatcgtcgta
     1681 cctgagtatc agagtcagac gcagccagcc gaggtcatgg agccgcgccc ggcaaccacg
     1741 tcagtcatgg gctgatctgc gcctcttgcc agtggtgccg gcgcccacct actgggttca
     1801 gccggttgcg gcgcgcgcgg gattcaccgt gaacggctct gacgcttgtg cgtcgtgggc
     1861 ttgaactgcg tgcggcaact cgacaacgga ggcgccggtg agttcggttg ctgcgtcgct
     1921 ttcggtcgca gcgctcagct gcacgacctg aacccgtcgg acgacggccc gcggggcatt
     1981 ggtgagtcct tgttcgaaat ggtcgagcag ggcgagcagg gtgaagaaga agcacggcag
     2041 gccgatgaca gtcgccacca ggaggatgcc gacctggaca aggccgccgc ccactgccac
     2101 ctcctgagtc gatcgtggtg tccgactgta cggatcttcg ccgttcacgc tcgcagccaa
     2161 acgcattttg ccgcctcgtc ggcgtgtcgc cgcgactggg gttcgaaaga cgggccgttg
     2221 ggcccgcacg cgcgaaaatc ggcgcgttag tgatctgtct cgtcccacgg tccgtcgatg
     2281 tggcggcaac cgtgcccggg ccgaggcgac cgggtgtgcc cagcgcacgt gcacggctgg
     2341 ccgtggggcc agtcgaccgg catcgtgagc cacacctctt tgccgccagc ccgttcggat
     2401 acgccccact cgtcggccag cgcgtcgacg atgagcagac cacgcccgcc ggcgacacta
     2461 ccgctcgcgc cgacgctcat gatcgggtgg cgttcgtctg tgtcggaccc caatccccgt
     2521 gatggcaccg cgtcgagatc ggcgagcagg tcgagccgta ccggccgcaa gaccggcggc
     2581 ctgatgtctt tgtcaccgac gcacagcacc agggtgtgat ccgacacaca aagctccacg
     2641 tcgatgtggc cgcgggcgta gagaaccgcg ttcgtgacca gttccgacac ggccagggtc
     2701 agctcgtcgg tgacgttgct gatgccccat cgtttgcagg tgctcgcgac gaatcgccgc
     2761 gcctcgccgg cggcttgcgg cgtgggctcg aggcctagga gcgcgcggcg gcacatcgtg
     2821 cgatcgcgtt ggtcgagttg ttcatcagtg tcgccatgcc cggtccggcg gctggctaag
     2881 cctgccgagt gtcaacgcgc ggctaggacc aaaccgacgg cgactggcac gaacgaccac
     2941 gagagcacct tgagcgcgat ctcgaggggg cgcagcaggt caacggcggt gagggtgacc
     3001 ggcgtcccgt ctgatgccgt tgctgtaagg cggacgtcgc gcactgaccg gcgcaaccat
     3061 cgcgcaggag tgctcagtgt gcgctgcgcg aagatctgcg cggccgcacc gagagcgacc
     3121 agaaccgccg cggccgacat cgtccagtgc tgggcgtagt agccggtgag tacagggaaa
     3181 gccgcccatg acagcgaaag cccgaccgcg ttgtgcagga aacctccgaa ccactcgagg
     3241 ttgtagccca gggcgagaaa cgcgccgacg gcaatgaagg gcaacagccg caggccgccg
     3301 accagccagc catcgaccgc ggccgtgagg atcgcaacgc cgccgactgt cgcaagtgcc
     3361 cagctcgcga tgccggtgcg cagcgggccg ccgtgcagtt cgtcgaaagc gtgggcaccg
     3421 atgccgaggc caagaaagaa gccgagcaag ctccagccga ggcggtcgag gcgaatcggg
     3481 tgtgccagcg ccgagccgat tgcgacgtac gacaggttcc atgcggtgta gggcgggtgc
     3541 agcagcgaca accagtcgcg ccagccgccg tcgccgcggc cgcgactgta gaacgcgggc
     3601 ttggccggcg cctcagccac cgtcggcccg cgtcgcccac atcaccaggc cgccgccgaa
     3661 actcatccgt tgcacccgca cgtcggccag gcccgcggcc cgccagttgg cgacgtgagc
     3721 accgaccgga tgccgcaggt agtgatagct gatgttcggt ccgaggaatc tgccgacgcg
     3781 ataccaagcg cgcccgccgg tcacggcgcc cgcggccggc agtacgaacc gggtgtagaa
     3841 ccaccacacg acccgtgccg gagtccaggc cggcacatgg aattcgaggc tggcgatggt
     3901 gccaccgggg gcaaggcagc gggcaagctc ggcgatcgtg gtggccggat cgtcgacgta
     3961 ccgcatgagg tagctgaacg aaatggcgtc gaaagtgccg tcagggaaag gcaattcctc
     4021 tgcacgggcg accgcgagct tgatctgttc gcccgcaccg gcgaggcgga cgttggtcag
     4081 gccctgtcgc agcatgtctt ctgtcagatc gattccgacg agtttggcgt cgcacgcgtt
     4141 ggcaatcgcg agcgtgactc cggctggtcc gcacgcgacg tcgagcaccc gcttcggccg
     4201 agctgcggtg acccgctcga cgaccgcagc ccgccaccgc ctgtcctgac cgaacgaaag
     4261 gaggtaggcc aacgtgtcat agcgggaagg tagatcttga aacagcgagc gcgcgaagct
     4321 gttcagctcc gtcggcgctc gatctgtgcc catactcgct ccaggagttg ctgcgtgagg
     4381 tctttgtcgg ttgagatcgg gagccagcgt acggacctgg gcacggtatg aggaggtggc
     4441 gtgacagctt ggcgtgatct tgtcgaccaa gcggccggtc aactcggccc ggtctccccg
     4501 actctggcgc gccacctgcg caccgagctc gacgaggtcg agcagcgcgt cgggttcatc
     4561 gagcggtcag ccccgcggcg ctcgacgcgc gacggcttgc atgccttggt cgcgctgacc
     4621 agcgcgcggc tggccgacac ctgggatccc ggcaagttga gcgacgtcgc gacggcggtc
     4681 gagctcgcct accgcgcctc gcttcaccac cgggtcgtca gcgacgtgaa cccagccggt
     4741 ctccggtctc ccaatactcg ggtggtgctc gacggcgact ggtcgatcac gcaggcggcc
     4801 gtgctggtgg ctgacatcgg tccgtcggcc taccggattc tggtgcgcgg gtacggtgcg
     4861 acgcaggtcg gtcagttgtg cggcgtcggt gatccgcttg gcctgatgcg cgcggcagtt
     4921 gagctcgggg cgctggtcac tgaagacggt catggcgacg cggcgcaacg agtgtggcgc
     4981 gacgcggcgc aggggctgtg gcgcgacgcc agcctctcgc cgtcccgccg atcaacacca
     5041 ggcgccgtgc tggcctgggc ggtcgcggtt cagtcagctg agggcaccaa gaaggcgcgc
     5101 aaagtcgcgg cggccggcgc tgccgacgtc catccttctt cgaggtcgac caccacgacg
     5161 cctgccgtcg gcaggtcgta gcgcagccgc tccggggttg cgtcaccgcc gagcagcagt
     5221 agcgcaagtt cgtggaagcc cggattgtga ccgcaaatga gcaccgtctc ggcgctcgcg
     5281 ggtagcttcc ggacgatctt cagcagccgg gcggcgtcgg cgaggtacac cttgctctcg
     5341 agcttcagcc gggccgatgc gatcaccggt gcggcggcgg cgagcttctc gacggtttcg
     5401 cgggtgcgcg ccgaggtcga gcacacaacg agatcgagcg gatgtccgtc gagggcttcg
     5461 gtgatgctgc gcgcgacccc gggaagaacc gcaatgccgc gcggcgccag ttgccgctcg
     5521 tggtcgccgc cgctcgcagt cggtggatgg gtcacggcct tggcatggcg cacgatgaag
     5581 agtcggtgca tcagcacagt ctggccgttc agatccgaga gtgcatggcc gatgtactgg
     5641 tgctgcccgg atcgggagcc cgtgtccccg ggcctcacct acagccgtcg cggagggatc
     5701 ggttcgccga agccgcgacg agcttctcgc cgacaggcga ccggctcccg attcgggcag
     5761 cgccaaccca acctgcaccg tcacccacac ggccgtgtga agttgcgcca tttgacgcat
     5821 cgacggcgcg cattcgggcg cacaccgcag gcggctccgg tcgacattta agacatgcgt
     5881 agtgacggca gcaagggccg ccaggcgacg gctgcgccgc ggcgtcggcg gcgcgttccg
     5941 ctattcgccg tgtatgcggc cgcatcgctg gtacccgtgg ccgctctggg cgccgtcctc
     6001 gctgtgacct accgcgccga caccaacaac agcgcaatgg cagaggcacg cagtcaggcg
     6061 tccctgctcg ccgacgacgt tgtcggtccg cagctcggtg cctctccact tcagggctcc
     6121 attccggcga gcgccgggcc ggccatggct gcggtcttca gccgggcgtc gggtgagaac
     6181 cgcctgctca ggctccgcat ccgcgacatg tcaggcgagg ttgtctgggc agatgacggg
     6241 agccgcaaca ccgccgaagc tcccggtgac gaggtcctcg acgcggtgca cggcggcgtc
     6301 gtcagtgacc tcaccgaact gaactccgat ccaggcgaca ccggcccggt tggtccgaag
     6361 gtcgtcgaga tctaccagcc gatcgacgtc ggatctgctc gcacagaggt cggtgtgctc
     6421 gagatgtacc tgccgtacgc gcccatcgaa gcagacgtga cggctggcct gcaccgaatg
     6481 tatgagctgc taggcgcggg ccttctcgca ctctggctgg tgctcgccgg catttccgcg
     6541 tcgaccaccc gccgcctgcg caagcgcgcc gagcgactcc aatacatcgc gcatcaccac
     6601 gtcgcgtcag gacttttgaa cgcggaagga ttctccgacg ggctgcagcg tcaccttcac
     6661 gctgacgcgg atcacgaagc gtttgctcct gcagtcgcgc tgatcgacgt ctcccgcttc
     6721 cgcgagatca acgaggcgct cgggcgagct agaggcgatg aagtgcttct cgagctcgga
     6781 cgccgattcg cggccgtcgc tggtgagggt gcgctcgtcg cgcgcctggg tggagacgag
     6841 ttcgcggtgg cgagcatcac cgatcacgac gccgacctgc acgactgggc gctgcgactc
     6901 caggccgctg ttgctgaacc ggtacaggct gatggcattc cggtcttgat cgaggtggcc
     6961 gtcggctact accgcagcga gccggggcag ccggtcgacg acctgatcgt gcacgccgac
     7021 gccgcgcttg gccgggccaa gtcgtcggtc gacaggtgcg ccggctacgt cgatcggccc
     7081 gatagtgcgg cgtcgagtgt ggccctgcta ccgcgtttcc gccgcgcgat ttcggcgggc
     7141 gaactcgagt tgcactacca gccgaagacc aagctctccg acggaactgt cgtggcgatg
     7201 gaggcactcg tccgctggaa tctggacggc gagttgttgc cgcccggcgc attccttccc
     7261 tcgatcgagc agacggcgat gatccacccg ctcaccgact gggtggcgcg ggcggccctg
     7321 gcccagctcg ccgagtgggg ccccgcgacc tcggggttgg aggtcgccat caacgtgtcg
     7381 gctcgcaatg tgtccgatcc tggctttgcc gcgaggctgc tagggatcgt tgccgagtcg
     7441 ggcgtcgagc cgcaccggtt gacggtcgag atcaccgaga cagcgctgtt cgtcgacccc
     7501 gacgctgcgc ggctgtgcct tggcacgctg cacgacgcgg gcatcgcgtt gtcgatcgac
     7561 gacttcggcc aggggcaaac gtcgcttgcc taccttgcag cgctt
//
LOCUS       DASWVF010000409         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_469702, whole genome shotgun sequence.
ACCESSION   DASWVF010000409 DASWVF010000000
VERSION     DASWVF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469702"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..387)
                     /locus_tag="VGD55_07405"
     CDS             complement(184..387)
                     /locus_tag="VGD55_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0870209.1"
                     /translation="MAQGTVKWFNAEKGFGFISPDGGDADVFVHHSQIQTNGYRSLDE
                     NQRVEFEVTQGPKGPQAEQVRPV"
     gene            complement(723..905)
                     /locus_tag="VGD55_07410"
     CDS             complement(723..905)
                     /locus_tag="VGD55_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870210.1"
                     /translation="MKLRSLVNLRRDLVIALAVPVAGKLMSVAAEQLRQRRGPNQVAD
                     RLDQASQLVRKVSRLV"
     gene            1155..2168
                     /locus_tag="VGD55_07415"
     CDS             1155..2168
                     /locus_tag="VGD55_07415"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY0870211.1"
                     /translation="MTAQSVDDTRRASDYAKLSRIVKDAGLLERRKLYYWARIALNVA
                     MVASAGFALYLANHTWWVLLVAVYWAIVSTQLAFTGHEAGHRQMFRSRQANELAGYLH
                     GNLMIGMSYDWWVDKHNRHHRNPNQAELDPDIGTGAFAFSREAAASRGRFGRFAARWQ
                     SLYFFPLLTLEALNLKIASVRAVATTGARNRGVEAVLLFIHLTGYVTLLLLTMSPLQA
                     LAFLGVHQALFGVYLGCSFAPNHKGMKMLTKDEEMDFLRRQVLTSRNVTGGVPVGMLL
                     GGLNWQIEHHLFPSMPRPNLRRAVPIVREFCESHGLPYAEASLYGSYRLAMGHLHDVG
                     VST"
     gene            2212..2457
                     /locus_tag="VGD55_07420"
     CDS             2212..2457
                     /locus_tag="VGD55_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY0870212.1"
                     /translation="MCRSIKTLRPPYVDEATSEDVTAAALQYVRKIAGMRKPSTANQQ
                     AFDEAVAEIAAATEHLLGHLQLRPAPVRRLEQPTSVG"
     gene            complement(2492..>2875)
                     /locus_tag="VGD55_07425"
     CDS             complement(2492..>2875)
                     /locus_tag="VGD55_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0870213.1"
                     /translation="VVGDLTPDMAMYAEEVFGPVAGLYRVSSREEALEVANGTEFGLG
                     SNAWTNDPAEQEFFIAGLDAGAVFVNGMTTSFAQLPFGGVRNSGYGRELSSHGIKEFC
                     NIKTVWISDGSPATSNTSSAGDHSE"
BASE COUNT          510 a         1010 c          881 g          474 t
ORIGIN      
        1 agatggtcga ggctgggatc gcccgcgagg agttcggcga gtcgtcctcg gcggcgctgt
       61 tcagccaccc gcacggcgtc cccgacatct aagtccccga ccccacccgg ccggcggaca
      121 aacagaaaca gcggcggtgc cggcgtttcc gccgagcacc accgctgtct cgttaatcca
      181 agattagacc gggcggactt gctccgcctg cgggcccttc gggccctgcg tgacctcaaa
      241 ctcgacacgc tggttctcgt cgaggctgcg gtagccgttc gtctggatct gcgagtggtg
      301 gacgaatacg tccgcgtctc cgccgtcggg gctgatgaaa ccgaagccct tttccgcgtt
      361 gaaccacttc acggtgcctt gcgccatctg gggtactcct cattaggtgt gctacgagcc
      421 agccacatcg gcggactcgg gctgccacgt catagttgcc gggagttgga gcgcgcaaac
      481 gcttcgcgaa tgcacgatct ggcccaaaca aaagacgccc gctgcgagaa tccgcgggcg
      541 tcatgaacgt tcgaggagta ctgcaactgc aaccgctagc caccctagca cggggacgcg
      601 cacccgaccg aactccggac gccgaaccct cggagggctc cggtcgctca agcggtcacg
      661 aggcgacggg agaacgcctc acgcactcaa gcgcctcaca cccctgacac ccctgacacc
      721 cctcacacca ggcggctgac cttacggacg agctggctgg cttggtcgag gcggtcggcg
      781 acctggttgg ggcctcgacg ctgccgcaat tgctcggcag cgaccgacat cagcttgccg
      841 gcgacgggca cggcaagcgc gatcacgagg tcgcgcctca ggttgaccag gctccgcagc
      901 ttcacccgag gcagtctacg agacgtgccc cgtcctcgaa ggccgcagcg caccccgtcg
      961 tctaacagcg cgcgcggcgc gccccgtcgt ctaagagcgc gcgcggcgcg ccagccaagg
     1021 tcggacggat ctggacgcgg cccacccctg gcgtgagaca cttccggcag gcggagcagt
     1081 ggctccccga acccatatcc gccatccgga ccccgtggcg atacctcggc gcgcgcgcct
     1141 ggaacggaag tgccttgacc gcacaatctg tcgacgacac ccgtcgcgcg agcgattacg
     1201 ccaagctgtc gcggatcgtc aaggacgcag gcctcctcga gcgccgcaag ctctactact
     1261 gggcacgcat cgcactgaac gtcgccatgg tcgcctccgc cggcttcgcg ctctacctgg
     1321 cgaaccacac ctggtgggtg ctgctcgtcg ctgtgtactg ggcgatcgtc tcgactcaac
     1381 tcgcattcac cggccacgag gccgggcatc ggcagatgtt ccgcagccgc caggcgaacg
     1441 agttggccgg ctacctgcac ggcaacttga tgatcggcat gagctacgac tggtgggtcg
     1501 acaagcacaa ccgtcaccac cgcaatccca accaagccga actcgacccc gacatcggca
     1561 ccggcgcttt cgccttcagc cgcgaagctg cggcgtcgcg cggccggttc ggcaggttcg
     1621 cggcccgctg gcagtcgctc tacttcttcc cactgctcac gctcgaagct ctcaacttga
     1681 agatcgcgag cgtgcgcgcc gtcgcaacga ccggtgcacg caatcgcggc gtcgaggcgg
     1741 tcctgctgtt catccatctg accggatacg tcacgctctt gctgctcacg atgtcgccgc
     1801 ttcaggcgct cgcgttcctc ggcgtccacc aagctttgtt cggcgtctat ctcggttgct
     1861 cgttcgcgcc caaccacaag ggcatgaaga tgctgaccaa ggacgaggag atggacttcc
     1921 tgcgccggca agtcctgacc tcgcgcaacg taaccggtgg tgtgccggtc ggcatgctgc
     1981 ttggtggcct gaactggcag atcgagcacc acctgtttcc gtcgatgccc cggcccaacc
     2041 tgcggcgcgc ggtgccgatc gtgcgcgagt tttgcgagag ccacggactc ccctacgccg
     2101 aggcctcgct gtacggctcg taccggctcg ccatggggca cctgcacgac gtgggcgtca
     2161 gcacctagat cacgatttcg cctgtcggcc tcgcgtcgta ggctggccgc catgtgccga
     2221 agcatcaaga ccctccgccc gccctacgtc gacgaggcga cctcagaaga cgtcacggcc
     2281 gcagcactcc agtacgtgcg caagatcgct ggcatgcgca agccgtcgac ggccaaccag
     2341 caagcgttcg acgaagcagt cgccgagatc gcagcagcga ccgaacactt gcttggccac
     2401 ctacaactgc gaccagcgcc ggtcaggcgc ctagagcagc cgacgtccgt cggttaaggc
     2461 ggctcgaaag cagaccgcaa gaagcaggcc gctattcgct gtggtcgccg gcgctgctgg
     2521 tgttgctggt cgcgggcgag ccgtcgctga tccagaccgt cttgatgttg cagaactcct
     2581 tgatgccgtg gctcgagagt tcgcgcccgt acccggagtt gcgcacgcca ccgaacggca
     2641 actgcgcgaa cgacgtcgtc atgccgttga cgaacacggc tcccgcatcg agacccgcga
     2701 tgaagaactc ctgctcagcc ggatcgttgg tccacgcgtt cgagccgagg ccgaactcgg
     2761 tgccgttggc gacttcgagg gcttcttcgc gcgacgagac ccggtaaagc ccagcaacag
     2821 ggccgaaaac ctcttcggca tacatcgcca tgtcgggcgt gagatcgcca accac
//
LOCUS       DASWVF010000410         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163652, whole genome shotgun sequence.
ACCESSION   DASWVF010000410 DASWVF010000000
VERSION     DASWVF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163652"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VGD55_07430"
     CDS             <1..2040
                     /locus_tag="VGD55_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0870214.1"
                     /translation="SVDFSMPGQPGYETAKKITAIFGPNSGFQAPTVAVVTVPAGTSV
                     TAQSATLETAFATIPQAVPGSRIVDYPNTHDEGLIGKDGRTTMVLIFAPLPKTFGVDP
                     ITGPTGDALKKSLPAGYTIGVTGLNQLASGGDSKGPGVLAETLFGAVGALAVLAFVFA
                     SLLAIIPLAVAAVSILSTFLVVLALTYITDVSFIVQFLVALVGLGIAIDYSLLLVTRW
                     REERDRGASNEAAVIRAVETAGHTVAVSGVTVAIGLIALVVIPVPGLRSVGIGGILIP
                     LVSIAVVLTLLPAILGGIGPRIDWPRVRHEQKASPAWSRWARLIVRRRWIAVGIAGAA
                     LAVAIAPIFGLKVGETSVQALAKTGPARVAYNELLAAGLSPGALTPIEVLTHSSATPA
                     TVAKLQAVKGITSVALPFGVQAHQPGYDDIIAIPASETVNNTTLAPVQGAKKAIAHAS
                     GVVGLTGMGAIEQDYSHAVFGNFPLMFALIAFATFILLVRAFRSLLLAIKAVLLNVIS
                     LAATFGLITWFWQEGHGSQTLFNIPATGAITFWIPLMIFAFLFGLSMDYEVFILSRMR
                     EEYDATGSTNHAVIEGIGRTGRLVTSAALILFMAFASLASAPDTDIKVLATALGAGIL
                     LDATVVRAFLVPALVSLFGRYNWWLPSWMATVLRVQPSPLMPKTFAADSIAYPGD"
     gene            complement(2025..>2402)
                     /locus_tag="VGD55_07435"
     CDS             complement(2025..>2402)
                     /locus_tag="VGD55_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0870215.1"
                     /translation="TLGTGIGSALFLDGELVPNTEFGHIEIDGQDAEIKTAESARERE
                     GLTWEHWAKRVSKYLRNLEAVLWPDLFIIGGGVSRKADKFLPLLEGVRTKVVAAELQN
                     DAGIIGAALWASEKLQTNGQSPG"
BASE COUNT          370 a          797 c          748 g          487 t
ORIGIN      
        1 tcggtcgact tctcgatgcc ggggcaaccc ggctacgaga ccgccaagaa gatcaccgcg
       61 atcttcggtc cgaacagcgg gttccaggca cctacggttg cggtcgtcac cgtgcctgcc
      121 ggcacgtccg taacggcgca atccgccacc ctcgagactg cgttcgcgac gatcccgcag
      181 gcagtgccgg ggtcacgcat cgtcgactac ccgaacacgc atgacgaagg actgatcggc
      241 aaggacggcc gcaccaccat ggtgctgatc ttcgcgccac ttccgaagac ctttggcgtc
      301 gacccgatca cgggcccgac cggtgacgcg ctgaagaaga gcctgcctgc gggttacacc
      361 atcggtgtga ccggcttgaa ccagttagcg tccggtggcg acagcaaggg ccccggtgta
      421 ctggccgaaa ccctgttcgg tgccgtcggc gcgctggccg tgctcgcgtt cgtgttcgca
      481 tcgctgcttg cgatcattcc gctggcggtt gccgcagtct cgattctctc gacgttcctc
      541 gtcgtgctcg cgttgaccta catcaccgac gtgtcgttca tcgtgcagtt cctcgtggcc
      601 ttggtcggcc tcggtatcgc catcgactac agcctcttgc tcgtgacgcg atggcgtgaa
      661 gaacgtgatc gaggtgccag caacgaagcg gcggtcatcc gagctgtcga gaccgctggc
      721 cacacggttg cggtatccgg cgtgaccgtt gcgattggat tgatcgcgct cgtcgtgatt
      781 cccgttcccg ggcttcgaag tgtcggcatc ggcggcatcc tgatcccact cgtgtcgatc
      841 gcagtggtcc tgacgctgct gcccgcgatc ctcggcggta tcggtccgcg cattgactgg
      901 ccgcgcgtca ggcacgagca gaaggcgagc cccgcgtggt cgcgttgggc acgtctgatc
      961 gtgcggcgtc ggtggatcgc cgtcggcatc gccggcgctg cgcttgccgt ggcgatcgcg
     1021 ccgatattcg gcctgaaggt tggcgaaacg tcggtacagg cactcgccaa gaccggcccg
     1081 gcgcgggttg cgtacaacga gctgctcgcc gcgggattgt cgcccggtgc actgacgccg
     1141 atcgaggtgc tcacgcattc gtcggcgacc cctgcgaccg tagcgaagct acaggcggtc
     1201 aagggcatca caagtgtcgc gctgcccttt ggcgtgcaag cgcatcaacc tggctacgac
     1261 gacatcatcg cgatccctgc ctccgaaacg gtgaacaaca ccacgctggc gccggtgcaa
     1321 ggtgcgaaga aggcgatcgc ccacgcctcc ggcgtcgtcg gcctgaccgg catgggcgct
     1381 atcgagcagg actacagcca cgcggtcttc ggtaacttcc ccctgatgtt tgccttgatc
     1441 gcgttcgcga cgttcatctt gctcgtgcga gcgttccggt cgctgttgct cgcgatcaag
     1501 gccgtgctgc tcaacgtgat ctcactcgcg gcgacgttcg gcctgatcac ctggttctgg
     1561 caggaagggc acggctcgca gacgctgttc aacatcccgg ccacgggtgc gatcacgttc
     1621 tggatcccgt tgatgatctt tgcgttcctc ttcggattgt cgatggacta tgaggttttc
     1681 atcctcagcc gcatgcgcga ggagtacgac gcgactgggt ccaccaacca tgccgtcatc
     1741 gaaggcatcg ggcggaccgg tcgccttgtc accagcgcgg cgctcattct gttcatggcg
     1801 tttgcgtcgc tcgcgtctgc tccggacact gacatcaagg tcctagcgac cgcgctgggc
     1861 gcgggcattc tgctcgacgc aaccgtcgtc cgcgcgttcc tggttcccgc gctggtgtcg
     1921 ctcttcggcc gctacaactg gtggctgccg tcgtggatgg cgaccgtact gcgagtgcag
     1981 ccgtcaccgc tgatgccgaa gacattcgcc gccgactcga ttgcctatcc cggcgactga
     2041 ccgttcgtct gcagcttctc cgatgcccag agcgcggcgc cgatgatgcc cgcgtcgttt
     2101 tgcagctctg ccgctacgac tttggtgcgc acgccctcaa gcaacggcaa gaacttgtcg
     2161 gccttccggc tgacgccgcc accgatgatg aacaggtcgg gccacaacac cgcttccagg
     2221 ttgcgcaggt acttcgagac gcgcttcgcc cagtgttccc aggtcaggcc ttcacgctcg
     2281 cgcgcggatt cggccgtctt gatctcggca tcctggccgt cgatctcgat gtggccgaat
     2341 tcggtgttgg gcaccagctc gccgtccaga aaaagtgcgc tgccgatgcc cgtgcccaat
     2401 gt
//
LOCUS       DASWVF010000411         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_367648, whole genome shotgun sequence.
ACCESSION   DASWVF010000411 DASWVF010000000
VERSION     DASWVF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367648"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1233
                     /gene="ugpC"
                     /locus_tag="VGD55_07440"
     CDS             151..1233
                     /gene="ugpC"
                     /locus_tag="VGD55_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY0870216.1"
                     /translation="MAEVEYRHATRIYPGTNTAAVSALDLDIHDGEFMVLVGPSGSGK
                     STALRMLAGLEAVDEGQIMIGDRDVTDVQPKDRDIAMVFQNYALYPHMTVAENIGFHL
                     KIKKRPKAEIKQRVLEAAKLLDLEPYLDRKPARLSGGQRQRVAMGRAIVRQPQVFLMD
                     EPLSNLDAKLRVATRTQIASLQRRLGVTTVYVTHDQVEAMTMGDRVAVLKDGLLQQCD
                     TPRNLFDRPVNVFVAGFMGSPAMNLIELPVTESGVTLSHAVMALTPAQRAALTGSQVT
                     IGVRPEGWDIVSPADGLAATVEVVEELGSDSYSYVTPQIDGGKTIVVRAANAATVPLK
                     GDVIGLRPKVEALHLFDTTSGLRLPD"
     gene            1269..>2421
                     /locus_tag="VGD55_07445"
     CDS             1269..>2421
                     /locus_tag="VGD55_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0870217.1"
                     /translation="MPDLVDLVRSHTTLADDDLDWLHLLVGEWQLLADLSFADLLLWV
                     RTAHSPGFVLVAQIRPVTAQTAYVVDMVGQVIADGKRALVDRAWREGRICREGDPEWT
                     EGIPVREETIPVRHDDRVIAVVARHTNLATVRTPSRLELTYLASAADLARMVAEGRFP
                     FPAGQVDSDSSPRAGDGLLRLDAEGRVTYASPNAVSAYRRLGLAGDVVGIHLGETTAK
                     LAPTAGPIDEPISWLVGGLSGLEADVEIEARGVALRLRTIPLLPGGERVGTLVLLRDV
                     TEVRRRERALLSKDATIREIHHRVKNNLQTVAALLRLQARRLDLPEARTALEEAIRRV
                     RAIALVHDTLSHTLDGPIDFDPVADRLIAMVGEVSAAESGALPRRVGSFG"
BASE COUNT          405 a          834 c          784 g          398 t
ORIGIN      
        1 cctgccgacg cgcgggacta atgacaccct ccgttaaggg tgttataact cgcagggaac
       61 gatcgcatgc acgagtgcga tcggttgcag caacgaaaca cgaaatgcgc atggatgctt
      121 gatcaccaca tcttccgagg agccccactg atggcagagg tcgaataccg ccacgcgacc
      181 cggatctacc cgggcaccaa cactgcagcc gtctccgcgc tcgacctgga catccacgac
      241 ggcgagttca tggtgctcgt cggaccgtcg ggttctggca agtcgaccgc ccttcgcatg
      301 ctcgccggcc tcgaggccgt cgacgagggc cagatcatga tcggtgaccg tgacgtcacc
      361 gacgtgcagc ccaaggaccg cgacatcgcg atggtgttcc agaactacgc gctctacccg
      421 cacatgacgg tggccgagaa catcggcttc cacctcaaga tcaagaagcg gccgaaggcc
      481 gagatcaagc agcgggtgct cgaagccgcc aagctcctcg accttgagcc atacctcgac
      541 cgcaagcccg cgcgcctctc cggcggccag cggcagcgcg tcgcgatggg tcgcgcgatc
      601 gtgcggcagc cgcaggtctt cctcatggac gagccgctgt cgaacctcga tgccaagctc
      661 cgtgtcgcca ctcgcaccca gatcgcctcg ctgcagcgcc ggctcggtgt cacgaccgtt
      721 tacgtcacgc acgaccaggt cgaggccatg acgatgggcg accgcgtcgc ggttctcaag
      781 gacggtctgc tgcagcagtg cgacacgccg cgcaacctct tcgaccggcc ggtcaacgtc
      841 ttcgtcgctg ggttcatggg tagccccgcg atgaacctca tcgagttgcc cgttaccgag
      901 agcggtgtca ccctgtcgca cgcggtcatg gcgctcaccc cggcacagcg cgccgcgctg
      961 actggaagcc aggtcacgat cggcgtccgg ccagagggct gggacatcgt ctctcccgcc
     1021 gatggactcg cggccacggt cgaagtcgtc gaagaactcg ggtccgactc ctactcctac
     1081 gtcactccgc agatcgacgg cggcaagacg attgtggtgc gcgcggccaa cgcggcgacc
     1141 gtcccgctga agggtgacgt catcgggctg cgaccgaagg tcgaggcctt gcatctcttc
     1201 gacaccacca gcggcctccg gctacccgac tagaagaccc ggcatccaga ggttcggcgt
     1261 tcttagccgt gcccgatctg gttgatctgg tccgaagtca cacgacgctg gccgacgacg
     1321 accttgattg gctccacctg ctcgtcggcg aatggcagct cctcgcggat ctgtcgttcg
     1381 ccgatctgct gttgtgggtg cgcaccgctc actcaccggg gttcgtgctc gtcgcccaga
     1441 tccggccggt gacggcgcag acggcgtacg tcgtcgacat ggtcgggcag gtgatcgccg
     1501 acggcaagcg ggcgctggtc gaccgggcct ggcgcgaggg ccgcatctgc cgcgagggtg
     1561 acccggaatg gactgagggc attccggtgc gcgaggagac gatcccggtg cgccacgacg
     1621 accgggtgat cgccgtcgtc gcgaggcaca ccaacctggc gaccgtgcga accccaagtc
     1681 gcctcgagct gacctatctc gcaagcgccg ccgatctcgc tcgcatggtg gccgagggca
     1741 ggtttccttt ccccgcagga caggttgatt ccgacagctc accgcgtgcg ggcgacggtc
     1801 tcttgcgatt ggacgccgag gggcgggtga cctacgcaag tccgaacgcc gtctcggcct
     1861 accggcgact cggcctggcc ggtgacgtcg tcggcattca cctcggcgag accaccgcca
     1921 agctggcacc gaccgccggc ccgatcgacg agcccatctc gtggctggtc ggcgggctct
     1981 cgggtctcga ggcagacgtc gagatcgaag cgcgcggcgt cgcgctgcgg ctgcgcacga
     2041 tcccgctgct gcccggtggt gaacgggtcg gcacgctggt gctgctgcgc gatgtgaccg
     2101 aggtgcgtcg ccgcgaacgc gcgctgctga gcaaggacgc gacaatccgc gagatccatc
     2161 accgggtcaa gaacaacctt cagacggtcg cggcactgct gcgcttacaa gcccgtcgcc
     2221 ttgatctacc ggaagccaga actgccctcg aggaggccat ccgccgggtc cgtgcgatcg
     2281 cgctggtgca cgacacgctc tcgcacacac tcgacggccc gattgatttc gaccccgtcg
     2341 ccgaccggtt gatcgcgatg gtcggtgagg tgtcggccgc ggagtccggc gctctcccgc
     2401 gtcgtgtcgg ctcgttcggg c
//
LOCUS       DASWVF010000412         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163670, whole genome shotgun sequence.
ACCESSION   DASWVF010000412 DASWVF010000000
VERSION     DASWVF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163670"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGD55_07450"
     CDS             <1..412
                     /locus_tag="VGD55_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0870218.1"
                     /translation="EMRYGAGRDIGGTVALLTLGTGIGSALFLDGELVPNTELGHLEI
                     DGQDAELKTAESAREREDLSWEHWARRLSKYLRHFEALLWPDLFILGGGVSRKSDKFV
                     PLLEGVRTKIVVAQLQNDAGIIGAALWASQAKGR"
     gene            449..1048
                     /locus_tag="VGD55_07455"
     CDS             449..1048
                     /locus_tag="VGD55_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEY0870219.1"
                     /translation="MPDNTTADSAQSELWADVDHSGYYPELMADSLRGTLGTEQALAH
                     VVHQETTLDDAMGIRRHVTVLATTPTRLIVCHTDEHPASELTPYPHATTTTEVVPLSR
                     IASVALTTMVANPAEYVPGASYREATLVVGWGAISHVDLEPATCGDENCEADHGYSGT
                     LTADDLSLRVSQDGDGAPVVTQAIRFATVLSEAAARARA"
     gene            1076..>1809
                     /locus_tag="VGD55_07460"
     CDS             1076..>1809
                     /locus_tag="VGD55_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0870220.1"
                     /translation="MPIPRYGSASLADLVPSVLASLGTPTFRGVLSLPPAMGACILLI
                     DGLGAELIRAHPADAPLLYAALTGDAGRDLTSGFPSTTAVSIGSIGTGLPPGAHGILG
                     YQVAIPGTTRLMNSLRWDPEVDPLSWQPLPTAFELAAAAGTTVVEVAKREFEGGGLDR
                     AVLRAPSFVGSDTFGEIAAGAIAQLSAAVAARRPGLVYAYVSDLDWTGHGHGVGSLAW
                     RLQLQLVDRLVEQIVDRLPMGARLYVT"
BASE COUNT          284 a          613 c          599 g          313 t
ORIGIN      
        1 ggagatgcgt tacggcgccg ggcgcgacat aggcggcacg gtcgcgctcc tgacacttgg
       61 taccgggatc ggtagcgcac tctttctcga cggcgagttg gtgccgaaca ccgaactcgg
      121 gcatctcgag atcgatggcc aggacgccga actgaagacg gccgagtcgg cccgcgaacg
      181 tgaagacttg agctgggagc actgggctcg gaggctgtcg aaatacctcc gccatttcga
      241 ggctctgctg tggcccgacc tctttatcct cggcggcggc gtgagccgca agtcggacaa
      301 attcgtgccg ctgctcgagg gtgtccggac caagatcgtc gtcgcgcagt tgcaaaacga
      361 cgccggcatc atcggcgcgg cgctgtgggc ttcgcaggct aagggccgct agccgccgtc
      421 aaggctggcg atagtggaag atggacccat gcccgacaac accacggccg attcggccca
      481 gtcagagctt tgggcggacg tcgaccacag cggctactac cccgaactga tggctgattc
      541 gctgcgcggc acgctcggta ccgagcaggc gctcgctcac gtcgtccacc aggagaccac
      601 gctcgacgac gcgatgggca tccggcgcca cgtcacggtg ctggccacca cgccgacccg
      661 tcttatcgtg tgtcacaccg acgagcaccc ggcgagcgag ctcacgcctt acccgcacgc
      721 gaccacgacg acggaggtcg tgccgctgtc gcgtatcgca tcggtggcac tcaccacgat
      781 ggtggcaaac cctgccgagt acgttcctgg cgcctcctat cgcgaggcga ctctcgtggt
      841 cgggtggggc gcgatcagcc acgtcgacct cgaacccgcg acgtgcggtg acgagaactg
      901 cgaagccgac cacggctact cgggcacgct caccgctgac gatctctccc tgcgggtcag
      961 ccaggacggc gatggtgccc ccgtggtcac acaggcgatc aggttcgcga cggttctgtc
     1021 cgaggcggcc gcgcgggcgc gggcctgact cgtgagtgcg gtccatccgg acacggtgcc
     1081 gattccacgg tacgggtccg catctctcgc cgatctggtg ccatcagtac tggcatcgct
     1141 tggcactccc acgtttcgtg gtgtgctctc gttgccaccc gcaatgggcg cctgcatcct
     1201 gctcatcgat ggacttggcg ctgaactcat cagggcgcac ccagctgatg caccgctgtt
     1261 gtacgcggcg ctcacgggcg atgccgggcg cgacctcacg tcgggtttcc cctcgacgac
     1321 ggcggtcagt atcggctcca tcggtaccgg tctgccgccc ggtgcgcacg gcattctcgg
     1381 ttaccaggtg gcaattcccg gcaccaccag gctcatgaac tcgttgcgct gggaccccga
     1441 agtcgatccg ctcagctggc aaccgctccc cacggcgttc gagcttgcgg cagccgccgg
     1501 gaccacggtg gtcgaagtcg ccaagcggga gttcgagggc ggcggtctgg atcgggccgt
     1561 gctgcgcgcg ccgtcatttg tcggatccga caccttcggg gagatcgcgg ccggcgcgat
     1621 cgcgcagctc tccgccgccg tcgccgcgcg gcggccgggc cttgtctacg cctatgtctc
     1681 cgacctcgac tggacgggtc acggtcacgg ggtcggctca ctcgcttggc ggctgcagct
     1741 ccaactggtc gatcggttgg ttgagcaaat cgtcgaccgg ctaccgatgg gggccaggct
     1801 ctacgtgac
//
LOCUS       DASWVF010000413        10842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_342230, whole genome shotgun sequence.
ACCESSION   DASWVF010000413 DASWVF010000000
VERSION     DASWVF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342230"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..2126
                     /locus_tag="VGD55_07465"
     CDS             147..2126
                     /locus_tag="VGD55_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0870221.1"
                     /translation="MEKSPGQLEDSPVGSGVRWAHYVAALLAAGYLVAVAARHVGSYN
                     ALVDGWGVDVFEVSVGVLCMLRFRHGSWRTITSSTRLFPLLLGAACITWGLGDTALTI
                     ESFGGATPPTPSAADGFGLMFFPLCYLALAVLLRRDTKGVAISAWLDRAIAGLGLAAV
                     AVAFVLHPVLKAVGGWSLASATSMAYPVGDLVLLAVVTGGFATASVARRQVLAVIGIA
                     MVVNFVGDTYAMLQPDSTVGNIANALAWPISILMLSAAAWIQPATTSTTSATSRRSGF
                     VLPGLGAAASLLILICASVGTISKDAVGFATATLFIAGIRLALTVQRAQSVNDERQRA
                     MTDRQEILLKVLTEVARNAQLLTAASERLTATATRLSGGADQASSQADVVANASRLIS
                     TSTQSVATGTEQMTASIAEIARNAADAASAGVEAMQETERTNTTISKLAKSSAAIGTV
                     VQVITTIAQQTNLLALNATIEAARAGEAGRGFAVVAGEVKELATETARATEEISSMIR
                     GIQDDTANSVEAIGRISQTIARINEIQISIASAVEEQRDDGAGNPDRGRGVARCRADQ
                     PSHLGCGRRRQGDGGWRERHPRGRIRAGHDGGDAAQPRIGVRGALASLIRPYYEAASF
                     IVSTPRSRLLRRRWLLRKACATRHEQPYLQHRRAW"
     gene            complement(2023..2820)
                     /gene="deoC"
                     /locus_tag="VGD55_07470"
     CDS             complement(2023..2820)
                     /gene="deoC"
                     /locus_tag="VGD55_07470"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0870222.1"
                     /translation="MESLWTGPVPDYSKPPQGELTVAQVAAMIDHSLLRPELTLHDVT
                     LGCEIAASHEVMSVCCKPADVVHASRLLAHSSVKVGTVVGFPHGSSSTETKVFEAQGA
                     LEDGARELDMVINIGRLRGGEHAYVAADIRAIVEVAATAGALVKVIFENVYLTDDQKV
                     LACHLTEEAGADFVKTSTGFAASGATMEDLALMRRSVGPAMQVKAAGGVRTLGTMLAM
                     VAIGVSRFGATATVTMLADAVDRAARGELRMPSAAAIDAEAVVTAEY"
     gene            complement(2951..4036)
                     /gene="ychF"
                     /locus_tag="VGD55_07475"
     CDS             complement(2951..4036)
                     /gene="ychF"
                     /locus_tag="VGD55_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0870223.1"
                     /translation="MALSFGIVGLPNVGKSTLFNALTKNDVLAANYPFATIEPNVGVV
                     GVPDSRLAALAALYSSAKIVPATVSFVDIAGLVKGASEGQGLGNKFLANIRESDAICQ
                     VTRVFTDPDVAHVDGRVSPKDDIETINTELILADLQTVDKALPRMEKESRLGGPTAKA
                     RAEVLEAVRHAGEVLNSGQTAFAAGLDPEPLRELQLLTSKPFLYVFNVDDEEMGNESL
                     KDELRSLVTPAEAIFLDAKIESELIELPDDEALELLQSIGQSESGLATLARVGFATLG
                     LQTFLTAGPKESRAWTIRRGATAPEAAGVIHTDFQRGFIKAEVVAYDDLMAAGSMLEA
                     KARGKVRIEGKEYVMADGDVVEFRFNV"
     gene            complement(4048..4599)
                     /locus_tag="VGD55_07480"
     CDS             complement(4048..4599)
                     /locus_tag="VGD55_07480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0870224.1"
                     /translation="MPIEIASGSLVIRSWTESDAGALGRAVAENLDHIRPWLPWARNL
                     PSDPGEVFEAMSRWIVEATAKPDEVVGLFVDGEVVGGSGLHPRIGAGGLEIGYWVDRR
                     FTRRGIATTASRSLTNHAFGQPGIDRVEIHHDKANIASEGVPRALGFALVAEVAVAPE
                     TPEQSGVHLIWRFTRAEWDRSGP"
     gene            4672..6102
                     /locus_tag="VGD55_07485"
     CDS             4672..6102
                     /locus_tag="VGD55_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0870225.1"
                     /translation="MTGTSLILLVLGLAVGLTIGYLRAQSRSTSRLATLTAELESTRA
                     HSARELEALRVSSDRELAIERANANDKLEAARLTEDRAREMFAALSSDALHKNSTAFI
                     ERADELIKRVTAESTGDLEKRQQAIELLVSPLKETLTNVEKQMKDVEKERTDAYAALR
                     TQVAQMGKTSDKLQQETSQLVAALRAPQTRGAWGEHQLRQVVEFAGMVEHCDFGEQQS
                     VSTADGMLRPDLIVNLAGGKHVVVDAKVSLVGFLDAMAVEEVGADPTAVAATRAHHLK
                     RHARHLRDHIDQLSAKAYWDLLPNTPEFVVMFIPAETFLNAALEQDPALFQYALEKNV
                     VIATPATLIALLRTVGYAWRQEALATNAHDVLTLGRELYSRLSTMGIHISKLGTKLNS
                     AVQSYNETVASLETRVFTTARRLVDLKVTSEELEAPRPIELVSRKLQRSELVASANDS
                     LVSLPAPQPQLLFEPVPLDPSEHATG"
     gene            6150..6866
                     /locus_tag="VGD55_07490"
     CDS             6150..6866
                     /locus_tag="VGD55_07490"
                     /inference="COORDINATES: protein motif:HMM:NF042451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HEY0870226.1"
                     /translation="MSEPAESLAEPFELLVGPAMPHVDLAARTATPVAHELDADELAD
                     EPDDEVLRPDVEAVEPGPVAADRAVSGRAVSGNVPASRVVSGRGVTGRGIVVMILLVT
                     GVVGLIEVTVAGHRGHAFAIAFVVTAAIGALVVRPRDLPTAIIAPPLLYSVLIVAMSA
                     IDRNDETGGLLTREGVYIGNAFVTGAPALWAGAAATVVIAWYRRRTSASRVAPAKPPK
                     PARPARPAMPVDEEAPPAKS"
     gene            6941..7399
                     /locus_tag="VGD55_07495"
     CDS             6941..7399
                     /locus_tag="VGD55_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870227.1"
                     /translation="MRVRLGALGVAAGVAVVSLVSACSASSAAQSSPVHGAHGSPSSV
                     VRDIDDTANGSTVHVHVGDVVRVTLHSTYWEMNAPSTTALQVGISDVVASPPAPGHVP
                     GLGAGTVVTSYVAHSSGTAQITAHRTSCGEALQCTPDKQSYAVTIAVDAS"
     gene            complement(7431..8405)
                     /locus_tag="VGD55_07500"
     CDS             complement(7431..8405)
                     /locus_tag="VGD55_07500"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0870228.1"
                     /translation="MTQAAASGKRVLLAQPRGYCAGVERAIDSVEKALAVHGAPVYVR
                     KQIVHNKHVVADLEAKGAIFVEETSEVPEGSIVVLSAHGVAPTVYAEATQRKLRTIDA
                     TCPLVTKVHHEARRFSDEGLEILLIGHAGHEEVVGTAGQAPDKVKLVDGPVDAARVEV
                     RDPSRVAWLSQTTLSVDETLETVHALRQRFPELMDPPSDDICYATQNRQAAVKQIAPA
                     SDLVIVVGSRNSSNSVRLVEVALENGARASHLVDDSSEIDETWLDGVVTVGLTSGASV
                     PDSLVLGVLAWLADRGFSEVEEIEAVRETLTFALPPELRRDIKAHAER"
     gene            8467..9690
                     /gene="xseA"
                     /locus_tag="VGD55_07505"
     CDS             8467..9690
                     /gene="xseA"
                     /locus_tag="VGD55_07505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0870229.1"
                     /translation="MALESSAESPIPVRSVAQAIGQWVGRLGRVWVDGQLTQITRRPG
                     TNTVFFVLRDPAADISLNVTAARQLIDGMADQLTEGSRVVVWAKPEFYLARGTLQLHA
                     VEIRPVGVGELLARIERLKRLLASEGLFDADRKRPLPFLPGTIGLVCGRESAAERDVL
                     ENAKRRWPAVQFRVEAVAVQGPYAVAEIVSAVHALDIDPQVDVIVIARGGGSVEDLLP
                     FSDEALVRAVAACRTPIVSAIGHEPDSPLLDLVADVRASTPTDAGKLIVPDVAEEQRR
                     ISLLRDRALRYVTTRIEREQDALRSLRARPVLATPERDVERRSRDVADLVARARRSLG
                     AGLDRAADDVVHLLARVRSLSPAATLERGYAVVQDAEGRVLRSATGVQTGAAIGVRLA
                     SGKLTAAVTSANTED"
     gene            9718..9993
                     /locus_tag="VGD55_07510"
     CDS             9718..9993
                     /locus_tag="VGD55_07510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0870230.1"
                     /translation="MAKAPDAEISHDSISYEAARDELLEVVRQLEAGGVPLEQSLVLW
                     ERGEQLAVLCQAWLDGAKKRLAARRPDPADAGVPVVSASTEAGGGSL"
     gene            9990..>10842
                     /gene="glpX"
                     /locus_tag="VGD55_07515"
     CDS             9990..>10842
                     /gene="glpX"
                     /locus_tag="VGD55_07515"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0870231.1"
                     /translation="MSAAETPDRNLALELVRVTEAAAMAAGRWVGLGEKNDADAAAVD
                     AMRRLIGTVAMRGVVVIGEGEKDNAPMLFNGERVGDGTGAEADVAVDPIDGTRLTAMG
                     MNNAISVMAVAERGSMYDPSAVFYMQKLVTGEAAAPFVDIEAPVAANIAAVAKAKGRA
                     NEDVTVVVLDRPRHEQLVSEIREAGARIKFILDGDVAGAIMAAREGTGIDLLLGIGGT
                     PEGIIAACAVKCLGGVIQAKLWPRDETERVKALDAGLDLDAVLTTDNLVHSDNVFFVA
                     TGITDGEL"
BASE COUNT         1878 a         3675 c         3495 g         1794 t
ORIGIN      
        1 ggcgttgggc ttttctggag tcatgtcgcg tcgaccgagc cctcaaggca ccccgtcggc
       61 gtccgatacg aacctgacga ttcgtccgat cgggtgagct aggcccgaca gcgttcgacc
      121 cgtacccaga ttggccacga acagcgatgg agaaaagtcc cggccagctc gaagacagcc
      181 cagtcggcag tggtgtgcgc tgggcacact acgtcgctgc gctgctcgct gccggctact
      241 tggtcgccgt tgccgcccga cacgtcggct catataacgc gctcgtcgac ggctggggag
      301 tcgacgtttt cgaagtgtct gtcggcgtct tgtgcatgct ccgcttccgg catggctcgt
      361 ggcgcacgat cacgtcgtcg acgaggctgt tcccgctgct gctcggggct gcgtgcatca
      421 cgtggggcct gggcgatacc gcactcacga tcgagtcgtt cggcggagcg accccgccaa
      481 ctccgtccgc cgccgacggc ttcggcctca tgttctttcc gctgtgctac ctcgcgctcg
      541 ccgtgctgct gcggcgcgac accaagggcg ttgcgatcag cgcgtggctc gaccgcgcca
      601 tcgccgggct tggattagca gcggtcgccg tcgcgttcgt tctgcacccg gtgttgaagg
      661 cggttggcgg ctggtcgctg gcatcagcga ccagcatggc gtaccccgtc ggcgatctgg
      721 tgcttctcgc ggtcgtcacg ggcggcttcg caacagcgtc ggtggcgcgc cgacaagtac
      781 tcgccgtcat cggcatcgcg atggtggtca acttcgtcgg cgatacctac gcgatgctcc
      841 agcccgacag caccgtcggc aatatcgcca acgcattggc gtggccgatc tcgatcttga
      901 tgctgtcggc ggcggcctgg atacaaccgg cgacaaccag cacgacgagc gcgacaagca
      961 ggcgaagcgg ctttgtgctg cccggcctcg gtgcagcggc gagcctgctc atattgatct
     1021 gcgccagtgt cgggaccatc agcaaggatg ccgtcgggtt cgcaacggcg acgcttttca
     1081 tcgcgggcat caggttggcc ctgacggtgc aacgagccca atctgtcaac gacgagcgcc
     1141 aacgggcgat gacggatcgc caagagatcc tgctcaaagt actaaccgag gtcgcccgca
     1201 acgcgcagct gctgaccgcg gcgtccgagc gcctgaccgc tactgcgaca aggctcagcg
     1261 gcggcgcaga tcaggcgtca tcacaggcag acgtggtggc caacgcgagt cgactcatct
     1321 cgacgagcac ccagtcagtg gcaactggca cagagcagat gacggcatcg attgcggaga
     1381 tcgcgcgaaa cgccgccgac gccgcctcag ccggcgttga agccatgcaa gagaccgagc
     1441 ggacgaacac caccatcagc aagctcgcga agtcctccgc ggccatcggc accgtcgtgc
     1501 aggtcatcac gacgatcgcg caacagacga acctgctggc gctcaacgca acgatcgagg
     1561 ccgcccgagc tggcgaagct ggtcgaggat tcgccgtcgt ggccggggaa gttaaggagc
     1621 tcgcgacaga aacagccaga gcgacagagg agatcagttc gatgatccga ggaattcaag
     1681 acgacactgc aaactcggtc gaggcgatcg gccgcatcag ccagacgatc gcacgaatca
     1741 acgagatcca gatttccatc gcgtcagcgg tcgaagagca gcgtgacgac ggtgcaggta
     1801 acccagaccg tgggcgaggt gtcgcaaggt gccgagcaga tcagccgtca catctcggtt
     1861 gcggccgacg ccgccagggg gacggcgggt ggcgcgaacg acaccctcgc ggccgcatca
     1921 gagctggcca cgatggcggc gacgctgcac aacctcgtat cggagttcgg ggcgctctcg
     1981 cgagcttgat tcgtccgtat tacgaggccg cgagcttcat cgtcagtact ccgcggtcac
     2041 gactgcttcg gcgtcgatgg ctgctgcgga aggcatgcgc aactcgccac gagcagccct
     2101 atctacagca tcggcgagca tggtgaccgt ggcggtcgct ccaaaccggc tgacgccgat
     2161 cgccaccatc gcgagcatcg tgccgagcgt gcgcacacca ccagccgcct tgacctgcat
     2221 tgccgggccg acagatctcc gcataagggc gaggtcttcc atcgtcgcgc cagacgcagc
     2281 gaaaccggtg cttgtcttga cgaagtcggc gccggcctcc tcggtcaggt gacaagcaag
     2341 caccttctgg tcgtccgtca gatacacgtt ttcgaagatc accttcacca acgcacccgc
     2401 cgttgcggcg acctcgacga tcgcgcggat gtcggccgcg acgtacgcat gctcgccgcc
     2461 acgcagccgg ccgatgttga tcaccatgtc gagctcgcgc gcgccgtcct cgagcgcacc
     2521 ttgggcttcg aacaccttgg tctcggtcga actgctgccg tgcgggaagc cgacgaccgt
     2581 gccgaccttc accgaactgt gggcaagtaa ccggctcgcg tgcacaacat ccgcgggctt
     2641 gcagcacacg ctcatgacct cgtgcgaggc agcgatctcg cagccgagcg tcacgtcgtg
     2701 cagcgtcaac tccggccgca gcaacgagtg gtcgatcatc gccgcgacct gcgcgacggt
     2761 cagctcgcct tgaggcggct tggagtagtc aggcacgggt ccggtccaca gggattccat
     2821 gcgccgatcc tctcagccct accgcgacct cctccgtgag catcgcccgc tgaaggaatt
     2881 cctggatgtt ctcacgcccg gacagcccgc atttccttca ctcggcggca gaacgggtgc
     2941 gtcggactgc ctacacatta aaacggaact cgacgacgtc gccgtcggcc atcacgtatt
     3001 ccttgccctc gatgcgcacc ttgccgcgcg ccttcgcctc gagcatcgag ccggcggcca
     3061 tgaggtcgtc gtaagcaacg acctcggctt tgatgaaccc ccgctggaaa tcagtgtgaa
     3121 tgacgcccgc ggcctccggc gccgtggctc cgcggcggat cgtccacgcg cgcgactcct
     3181 tcggccccgc ggtcaggaac gtctgcaacc cgagggttgc gaaaccgacc cgtgcgaggg
     3241 tggcgagccc tgactcggat tgcccgatcg attgcaacaa ctccagcgcc tcgtcgtccg
     3301 gcagttcgat gagttctgat tcgattttgg cgtcgagaaa aatggcctcg gccggcgtga
     3361 cgagcgaacg caactcgtcc ttgagcgact cgttgcccat ctcctcgtcg tcgacgttga
     3421 acacatatag gaacggcttg gacgtgagca gttgcagttc gcgcagcggc tcgggatcga
     3481 gccctgcagc gaacgcggtc tggcccgagt tcaagacctc acccgcatgt cgaacggctt
     3541 cgaggacctc ggcccgcgcc ttagcggtgg gacctccgag ccggctctct ttctccatgc
     3601 ggggcagtgc tttgtcgacg gtctgaaggt cggcgagtat gagctcggtg ttgatggtct
     3661 cgatgtcgtc cttggggctg acccggccgt cgacatgcgc cacgtcgggg tcggtgaaaa
     3721 cccgcgtcac ctggcagatc gcgtcggatt cacgaatgtt cgcgaggaac ttgttgccga
     3781 gcccttgccc ctccgacgcg cccttcacca gcccggcgat gtcgacgaaa ctgaccgtcg
     3841 cgggcacgat cttggccgag ctgtacaacg cggccaacgc ggccaaccga gaatccggga
     3901 cgccgacaac gccgacgttt ggctcgatcg tcgcgaacgg atagttcgcc gccagtacgt
     3961 cgttcttggt cagcgcgttg aagagcgtcg acttgcccac gttcggcagc ccgacgattc
     4021 cgaaagaaag tgccacaacc agcgagtcta cgggccgctg cggtcccatt ccgcacgggt
     4081 gaaacgccag atgagatgga cgccgctttg ctcgggcgtt tcgggcgcga ccgcgacttc
     4141 tgcgacaaga gcgaacccga gcgcgcgcgg cacgccctcg cttgcgatgt tggccttgtc
     4201 gtgatgtatc tccacgcgat cgatgcccgg ctggccgaac gcgtgattgg tcaacgacct
     4261 cgatgctgtc gtcgcgatcc cgcgtcgggt gaaccgccgg tccacccaat acccgatctc
     4321 gagtcctccc gcgccgatcc gcggatgcag tcccgaccca cccacgacct cgccatcgac
     4381 aaacaggccc acgacctcgt ccggcttggc ggtggcctcg acgatccacc gactcatcgc
     4441 ctcgaacacc tcgccgggat cgctgggaag gttccgcgcc cacgggagcc acggccggat
     4501 gtgatcaagg ttctcggcga cggcacgccc cagcgcgccg gcgtcggact ccgtccagga
     4561 gcggatcacc agggatccgg acgcgatctc gatcggcatt cgccgaccct aaccgccgcc
     4621 ccggcgtggc acagccaggt tttgtcggcg tcccctgtca cgattcgaga catgacagga
     4681 acctcgctca ttctgctggt gctagggctc gcggtcggtc tcacgatcgg ctacctgcgg
     4741 gcgcagtcgc gatcgacgtc ccggctggcg acattgaccg ccgagctcga gagcacgcgc
     4801 gctcattccg cacgcgagct cgaggctctg cgcgtcagct ctgatcgcga gttggcgatc
     4861 gaacgcgcga acgccaacga caagctcgag gctgcgcggc tcaccgaaga ccgtgcccgt
     4921 gagatgttcg ctgcactgtc ttccgatgcc ctccacaaga acagcaccgc cttcatcgag
     4981 cgtgctgacg aactcatcaa gcgcgtcaca gccgagtcca ccggtgatct cgagaagcgg
     5041 cagcaggcca tcgaactgct ggtgagtccg ctgaaagaaa ccctgaccaa cgtcgagaag
     5101 caaatgaagg acgtcgagaa ggagcgcacc gacgcatacg ccgccctgcg cacccaggtc
     5161 gcgcagatgg gcaagacctc ggacaagttg cagcaagaga cgtcgcagct tgttgcagcc
     5221 ttgcgagcgc cgcagacccg tggcgcctgg ggtgaacacc agctgcggca ggtcgtcgag
     5281 ttcgccggca tggtcgagca ttgcgacttc ggtgagcagc agtcggtgag cactgccgat
     5341 ggcatgctgc ggcccgacct catcgtcaac ctggccggcg gaaagcatgt cgtcgtcgat
     5401 gccaaggtct ccctcgtcgg cttcctcgac gcgatggctg tcgaggaagt aggggcagac
     5461 cctacggccg ttgctgcaac gcgagcgcac cacttgaagc gtcacgcgcg acacctgcgc
     5521 gaccacatcg accagctcag cgccaaggcc tactgggacc tactgccgaa cacccccgag
     5581 ttcgtggtga tgttcattcc ggccgagacg ttcctcaacg ccgcgctcga acaggatcca
     5641 gcgctgttcc agtacgcact cgagaagaac gtggttatcg cgacccctgc gaccctcatt
     5701 gcactgctgc gcaccgttgg ctacgcctgg cggcaagaag ccctcgccac gaacgcccac
     5761 gacgtcctca ctctcggccg tgagctctac agccgactgt cgacgatggg cattcacata
     5821 agcaagctcg gtaccaagct gaactccgcc gttcagagct acaacgagac cgtcgcgtca
     5881 ctcgaaacac gcgtgttcac taccgcccgc aggctcgtcg acctgaaggt gacgagtgag
     5941 gagttagaag caccacggcc gatcgagctg gtgagccgca agctccagcg gtccgagctc
     6001 gtcgcgtcgg caaacgactc cctcgtttcc ctccctgcgc cccaacctca gctgttgttc
     6061 gagccggtcc ccctggatcc gagcgaacac gccaccggct gagcgcgtcg atagtggcac
     6121 cggcgcggtg cgcggcctag cgtgggcacg tgtccgaacc cgccgagtcg ctggctgagc
     6181 cattcgagct cctcgttggc ccggcaatgc ctcacgtcga cctcgcggcg cgcacggcaa
     6241 cgccggtcgc ccacgagctt gatgccgacg agctagccga cgagccggac gacgaagtgc
     6301 tgcggccaga cgtcgaggct gtcgagcctg gcccggtcgc ggccgatcgt gccgtgtccg
     6361 gccgagcggt gtctggcaac gtccccgcga gccgggtcgt atctggccgt ggcgtcaccg
     6421 gccggggcat cgttgtgatg atcctgctcg tcaccggcgt cgtcggcctc atcgaggtca
     6481 ccgttgccgg ccatcggggc catgcgttcg cgatcgcgtt cgtcgtgacg gccgccatcg
     6541 gagcactcgt cgttcgcccc cgcgacctgc ccaccgcgat cattgcgccg ccgctgctgt
     6601 acagcgtcct gattgtcgcg atgagcgcga tcgacaggaa cgacgaaacc ggtggactcc
     6661 tcacaaggga gggcgtctac atcggcaacg cgttcgtgac gggtgcgcca gccctctggg
     6721 ccggtgctgc ggcgactgtc gtcatcgcgt ggtaccgacg ccgcacctcg gcctcccgcg
     6781 tcgcgccggc caagccaccg aagccagcaa ggcccgccag gcccgccatg cccgtcgacg
     6841 aagaagcgcc accggcaaaa agctaaaact cgacggaacc gttcgctccc cccgtgcgtc
     6901 tgatgagcca cccagacggt ctcatgagga gtgaacgaca gtgcgagtac gccttggcgc
     6961 cctcggcgtt gccgccggtg tagccgtcgt gagcctggtc agcgcctgca gcgcctcctc
     7021 agcagcccag tcgtcgccag tgcacggcgc gcacgggtca ccgtcgtcgg tcgttcgcga
     7081 catcgacgac actgcgaacg gaagcaccgt ccacgtgcac gtcggcgatg tcgtcagggt
     7141 gacgttgcat tccacgtact gggagatgaa cgcgccgtcc acgacggccc tccaggtggg
     7201 catcagcgac gtcgtcgcgt cgccaccggc gcctggccat gtgccaggcc tcggcgcagg
     7261 cacggtcgtc acgtcgtatg tcgcgcacag cagcggcacc gcgcagatca cggcgcatcg
     7321 cacgtcctgc ggtgaggcat tgcaatgcac gcctgacaag cagtcgtatg cggtcacgat
     7381 cgcggtcgac gccagctgat cggcgtcccg cctgtgcgta taaaacagcg ctagcgctcg
     7441 gcgtgcgcct tgatgtcgcg ccgcaactcc ggcggcagag cgaacgtgag ggtttcgcgc
     7501 acggcctcga tctcttcgac ctcgctgaag ccgcggtcgg cgagccaggc caggacgccg
     7561 agcacgagcg aatccggaac cgacgcgccg ctggtgaggc cgaccgtcac caccccgtcg
     7621 agccacgtct cgtcgatctc gctcgagtcg tcgaccaagt gagaagcgcg cgcgccgttt
     7681 tcgagcgcca cctcgacgag ccgcacggag ttcgaggagt tgcgtgaccc gacaacgatg
     7741 acaaggtcgg acgccggggc gatctgtttc accgcggcct gccggttctg cgtggcgtag
     7801 cagatgtcgt cgctcggcgg atccatcaac tcgggaaagc gctggcgaag tgcgtgcacg
     7861 gtctccaagg tctcgtcgac cgacaacgtg gtctgcgaca gccaggccac gcgagacgga
     7921 tcgcgcacct cgacccgggc ggcgtcgacc ggcccgtcga cgagcttcac tttgtcgggt
     7981 gcttgaccag cggtgcccac aacctcttcg tggcccgcgt gaccgatcag cagaatctca
     8041 aggccttcgt cagaaaagcg ccgagcctcg tgatgcacct tggtgaccag tgggcaggtg
     8101 gcgtcgatcg tgcgtaactt tcgctgcgtc gcctcggcgt aaaccgtcgg cgcaaccccg
     8161 tgggctgaca gcaccacgat cgagccctcg ggaacctcgc tggtctcctc gacgaagatc
     8221 gcgcccttgg cttcgaggtc ggcgaccacg tgcttgttat gcacgatttg cttgcgcaca
     8281 tagacggggg cgccgtgcac agcgagcgcc ttctcgacgc tgtcgatggc ccgttcgacc
     8341 cctgcgcagt agccgcgcgg ctgggcgagg agtacccgct tgccggacgc tgcagcttgg
     8401 gtcatagctc catcgtaagt gccagccaga tcggccgcgg cgggtccgtc atttacgctc
     8461 accgctgtgg cactcgaatc gtcggcagaa tcgccgatcc cggtacgaag cgtcgctcag
     8521 gcgatcggcc agtgggtcgg gcggctcggg cgggtctggg tcgacggcca actgacgcag
     8581 atcacccgac gtccgggcac gaacaccgtg ttcttcgttc tgcgtgaccc ggctgccgat
     8641 atctcgctga acgtgaccgc agcacgtcag ctcatcgacg ggatggccga ccaactcacc
     8701 gagggcagcc gcgtcgtggt ctgggccaag cccgagttct acctcgccag ggggaccctc
     8761 cagttgcacg cggtcgagat cagacccgtc ggagtcggcg aactgctcgc gcgaatcgag
     8821 cggctgaagc gcctcctcgc ctccgagggc cttttcgacg ccgaccgcaa gcggccactg
     8881 cctttcctgc ccggcaccat cggactggtg tgtggccgcg aatcagcggc cgagcgcgac
     8941 gtcctggaga acgcgaagcg gcgctggccc gccgtgcagt ttcgggtcga ggcggtcgct
     9001 gtgcaaggtc cgtacgccgt ggccgagatc gtcagcgcgg tgcatgctct cgacatcgac
     9061 ccgcaggtcg acgtgatcgt gattgcccgt ggcggcggca gcgtcgagga cctactcccg
     9121 ttctccgacg aggccttggt acgcgccgtt gccgcctgcc ggacaccgat cgtgagcgcg
     9181 atcggtcacg aaccagactc gccgttgctc gacctcgttg ccgacgttcg tgcgtcgacg
     9241 cccaccgacg ccggcaagct gatcgtgccc gacgtcgccg aggagcaacg ccggatctcg
     9301 ttgctgcgtg atcgagcgct gcggtacgtc acgacccgca tcgaacgcga gcaggacgca
     9361 ctccgctccc ttcgcgcccg tcccgtgctc gcgacgccag aacgcgatgt cgagcgacgg
     9421 tcgcgcgacg tggcagacct cgtggcacgc gcgcgccgaa gcctcggcgc aggcctcgat
     9481 cgggccgctg acgacgtcgt ccacctgctc gctcgggtcc ggtcactctc gcctgcagcg
     9541 accctcgagc gcggctacgc cgtcgtgcag gacgccgagg ggcgggtctt gcgatcagcc
     9601 accggcgtcc agaccggtgc cgcgatcggc gtccgtctcg cctcggggaa gctgacagcg
     9661 gcggtgacct ccgccaacac cgaagactga gcaccacagg gccctacgct gatctccatg
     9721 gcgaaggcac ccgacgcgga gatctcgcac gactcgatct catacgaggc ggcgagggat
     9781 gagttgctcg aggtcgtgcg gcaactcgaa gcaggcggcg tgccgttgga gcagtcgctc
     9841 gtcttgtggg aacgcggcga gcaactcgcc gtcctgtgtc aggcctggct cgatggcgcg
     9901 aagaagcggc tggcggcgcg acgacccgat cccgccgacg caggtgtgcc ggtcgtttcg
     9961 gcctcgaccg aagccggcgg tggatcgctg tgagtgccgc cgaaacgccc gaccgtaacc
    10021 tcgcccttga actggtgcgt gtcactgaag ctgcggcgat ggcagccggc cggtgggtcg
    10081 gacttggtga aaagaacgac gctgacgcgg ccgcggtcga tgcgatgcgg cggctgatcg
    10141 ggacggtcgc gatgcgaggg gtcgtcgtga tcggcgaggg ggagaaagac aacgccccga
    10201 tgctgttcaa cggcgagcgg gtgggcgatg gcaccggcgc cgaggccgac gtggcagtcg
    10261 atccgattga cggcacccga ctgactgcca tgggcatgaa caacgcgatc tcagtgatgg
    10321 ccgtggccga gcggggatcg atgtatgacc cctcggcggt cttctatatg caaaagctcg
    10381 tgaccggtga ggcggcggca cccttcgtcg acatcgaggc gccagtcgcg gcgaacatcg
    10441 cagccgttgc gaaagcgaag ggacgcgcga acgaggacgt cacggtggtc gtgctcgacc
    10501 ggccccgcca cgagcaactg gtgagcgaga tccgcgaggc cggcgcgcgc atcaagttca
    10561 tcctcgacgg tgacgtcgcc ggcgccatca tggctgcccg cgaaggcact ggcatcgacc
    10621 tactgctcgg catcggcggc accccggaag gcatcatcgc ggcgtgcgcc gtgaagtgcc
    10681 tgggcggtgt gattcaggcc aagctctggc cgcgcgacga gaccgagcgg gtgaaggcgc
    10741 tcgacgccgg gctcgacctc gacgccgtgc tgaccaccga caacctggtc cactccgaca
    10801 acgtgttctt cgtcgccacc gggatcaccg atggcgaact tg
//
LOCUS       DASWVF010000414         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_367710, whole genome shotgun sequence.
ACCESSION   DASWVF010000414 DASWVF010000000
VERSION     DASWVF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367710"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VGD55_07520"
     CDS             <1..764
                     /locus_tag="VGD55_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870232.1"
                     /translation="RYRAAQSGGHMPPTTSATASAAASAATDPVVRFDQVTKTYRDGG
                     VKVEALRGISLAIAPRCFTMIVGPSGSGKTTLLNLIGCIDRPTTGRMEVCGRDLGALP
                     DDQITDFRAQNVAFVFQGFNLLPVLTAYENVEYPLLLLRVPASERRKRAVAMLEAVGL
                     TDYGHHRPNQLSGGQKQRVAIARALIKHPALVLADEPTANLDSRTGAEIIALMRRMQD
                     QQAISFVFSTHDPQLMSHADETFSIRDGRLAEALR"
     gene            761..>1637
                     /locus_tag="VGD55_07525"
     CDS             761..>1637
                     /locus_tag="VGD55_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870233.1"
                     /translation="MMTLKIAFRNILRNRRRSAMTLLAVAVGAMGVVLFGEFVRFVTA
                     GLETNAVQKVGHLTVFRTGYFAFGAGNPAEYGIDGYTNVVELIRRDRILAPRLNVVTP
                     IVSLAGIAGNFDLDASKTFMGTGVVPSDHDRLSRWDEHRVNRRRALVDSGLRDDDETR
                     GVVGVGLGRVLGFCERLHIGDCPPRPNAAAASGPALDGQLAGLALKERVPSAGTGRDA
                     SPQLDLLAATAGGAPHVVSMAVSRAEPQGARELDDNFIAMHIALAQKLVYGRMPAQAT
                     GIVVQLHHTADLPLAR"
BASE COUNT          270 a          538 c          551 g          278 t
ORIGIN      
        1 gacggtaccg cgctgcccaa tctggaggac acatgccacc gaccacttcc gccacagcgt
       61 cggccgccgc ttcggccgcc accgatccgg tcgtccgatt cgatcaggtc acgaagacgt
      121 atcgcgacgg tggcgtcaaa gtcgaagcgc tccgcggcat cagtttggcg atcgcgcccc
      181 gctgcttcac gatgatcgtc ggtccgtcgg gcagtggcaa gacgacgcta ctgaacttga
      241 tcgggtgcat tgatcgtccc accaccggtc ggatggaggt gtgcggccgc gacctgggcg
      301 cgctgccaga cgatcagatc acggacttcc gggcccagaa cgtcgcattc gtcttccaag
      361 gcttcaatct cctgccggtc ttgaccgcgt acgagaacgt cgagtaccca ctgctgctct
      421 tgcgcgttcc agcctctgag cgtcggaaac gcgcggtcgc gatgctggag gcagtcggcc
      481 tgaccgacta cgggcaccac cggcccaatc aactcagcgg cggccagaag cagcgggtgg
      541 cgatcgcgcg agctctgatc aagcacccgg cgctggtgct ggcagacgaa ccgaccgcca
      601 accttgacag tcgaacgggc gccgagatca tcgcgctcat gcggcgcatg caggaccagc
      661 aggcgatcag cttcgtgttc tcgacgcacg acccccagtt gatgtcccac gccgatgaga
      721 ccttctcgat tcgcgatggg cggctggcag aggcgctgcg atgatgacgc tcaagatcgc
      781 gttccggaac attctgcgca accggcgtcg atcggcgatg acgttgcttg cggtcgccgt
      841 cggggcaatg ggcgtggtgc tgttcggcga attcgtgcgg ttcgtgaccg ccgggctgga
      901 gaccaacgcc gtccagaagg tcgggcattt gacggtattc cgcaccggct acttcgcgtt
      961 cggcgcgggc aatccggccg agtacggcat cgacggttac acaaacgtgg tcgagttgat
     1021 ccgccgcgat cggatcctgg cacccagatt gaacgtcgtc acgcccatcg tttcgctggc
     1081 gggcattgcc ggcaacttcg acctcgatgc ctcgaagacg ttcatgggca ccggtgtagt
     1141 tccgtcagac cacgataggc tgtcacgctg ggacgaacac cgcgtgaacc ggcgccgggc
     1201 gctggtcgac tccgggttgc gcgacgacga tgaaacgcgc ggcgtggtcg gtgtcgggtt
     1261 gggccgggtg ctggggtttt gcgagcggct gcacatcggt gactgcccgc cgcggccgaa
     1321 tgcggcagcc gcgagcggcc cggcgctgga cgggcagctc gcggggttgg ccctcaagga
     1381 gcgcgtaccg tcggcgggga caggccgcga cgcgtccccg cagctggacc tgctggcggc
     1441 caccgctggt ggcgcgccgc atgtcgtgag catggccgtc agccgcgccg aaccgcaggg
     1501 ggcccgcgag ctcgacgaca acttcatcgc catgcacatc gcgctcgcgc agaagttggt
     1561 ctatggccgc atgcctgccc aggcgacggg aatcgtggtg cagctgcacc acaccgccga
     1621 tttgcctctg gcccgcg
//
LOCUS       DASWVF010000415         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_11248, whole genome shotgun sequence.
ACCESSION   DASWVF010000415 DASWVF010000000
VERSION     DASWVF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11248"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..899)
                     /locus_tag="VGD55_07530"
     CDS             complement(369..899)
                     /locus_tag="VGD55_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870234.1"
                     /translation="MLGAPHIPSHIKPQEMEQPGSGPLRQNPFAVVVTDAKGGAAATQ
                     AISTWLSWAGDGKPHPAPDGTGAYYFASGFPVNYVVIETGEGFPVIVIGPEVSLAADG
                     STPIGHPSLGVCPAGGGAALATIGGEIHGTTLNNHSGRYDYGPWVTADALNSAARLFN
                     CMGIQITDTKYYAPKS"
     gene            complement(1445..>2230)
                     /locus_tag="VGD55_07535"
     CDS             complement(1445..>2230)
                     /locus_tag="VGD55_07535"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEY0870235.1"
                     /translation="TDIATLRTLIDVPNTVLEWAAEAVVEDADGTYGGSHNFWIYDEG
                     TAGYVWLLDHTDSALEWLEVFTPSLGYKEHPIYWWAGRALPDPPAKNYLIVINDPTTR
                     AQYVNAIATQLAKWNASEIVGWIDIWSQQIAGAVAQDRHKWATMDQFEMAITAMKDMA
                     QNRPQYLHNFLACESGDASQATDQDGDGVAWCNDCDDSNPSVSPGVPEVCGNRVDDNC
                     NGVVDENCPRETPGYPGQPDGGVPPTATGADGGTTNVDGGASG"
BASE COUNT          378 a          737 c          696 g          419 t
ORIGIN      
        1 ggttcgcgtg ccttgtccgc ggcggccgcc gtctcgattc gaggtggttc gaccttggcc
       61 ggccgttgcc cgtcgggtat ggccacggcc ggcttgggcg aaggggcgcg acgctaggat
      121 agtactgtga gaatcagctc caagccgagt caagcacgcc accctcgact cgttctcgtc
      181 atcctcgcgc tcttttcggg atgctattac cgaggatgcg catgcgggcg ataccgagac
      241 cacgaccgcg ggcgcgacca ctaccacgag ttccacggtc acgatcacga tcgccggtag
      301 cgccgtatcg ctcgctcgca ggatccagcc cggtgccgtg cacaggatgg actctgcttc
      361 gcacgcggtc aggacttcgg cgcgtagtac ttcgtgtcgg taatctggat tcccatgcag
      421 ttgaacaacc tcgccgcgct gttgagtgcg tccgcggtga cccagggacc gtagtcgtag
      481 cggcctgaat ggttgttgag cgtggttccg tggatctcgc ctccgatcgt cgcgagggcg
      541 gcgccgccgc ccgccgggca cacgcctaaa gagggatgtc caatgggcgt gcttccgtcg
      601 gcggccagcg agacctcggg accgatgacg atgacgggga acccttcccc tgtctcgatg
      661 accacgtagt tgaccgggaa gcccgacgcg aaatagtagg cgcccgtgcc gtccggagcc
      721 ggatgtggct tcccgtcgcc tgcccatgac agccaggtcg atatggcctg ggtcgccgct
      781 gcgccaccct tcgcgtccgt gacaaccacc gcgaacggat tctgccgaag gggcccgctg
      841 cctggctgct ccatctcctg cggcttgatg tgcgacggta tgtggggtgc accgagcacg
      901 ccgacgctcg tttcaggcac cgtcggcgcg gccgacggcg agatcattcc gccgcagcaa
      961 ccctgcgcgt ccgacaggaa ggtggccggt tggccgaaga tgtatccgat ggacggatcg
     1021 ggagaccatg acgttgcgca tacgtagatc ggcgacgacc cagcgctgtc gatctccagg
     1081 taccccgtct gcgacggttg tgccccatcc tggctgcacg gaaacacctt tccggtggga
     1141 acggccgaac aggtgtccgc cggcgcggtc ggaaggacgc acgcagctgc gtccctcccg
     1201 ttgaccggcg tctgaccggg gccagcgtca gacgcgctcg aggcgggggc tccgccgtct
     1261 gaatggggag acgcagacga accgtcggcc agcccactcg acgtctgtgt actgcttccg
     1321 caggccatcg acaccagtgg gagaaacgcc agaacccatc ggacacacac cactcgtctc
     1381 atcgagagca tcccctgttc ccgaacgtag tttcgttcac cggccaacgc tggtcatgtg
     1441 ccaatcagcc cgatgcgccg ccgtcaacat tcgtcgtgcc cccatccgcg cccgtggccg
     1501 tcggtggtac gcccccgtct ggctgcccgg ggtagccagg ggtctcgcgc ggacaatttt
     1561 cgtccacgac accgttacag ttgtcgtcca cgcggttgcc gcaaacctcc ggcacgccag
     1621 gggagaccga cggattcgaa tcgtcgcagt cattgcacca cgcaacgccg tcaccgtcct
     1681 ggtccgtcgc ctgcgatgcg tcgccacttt cacaggcgag gaagttgtgc aggtactgtg
     1741 gccgattttg cgccatgtcc ttcatggcgg taattgccat ctcgaactgg tccatcgtgg
     1801 cccacttgtg ccggtcctgg gcaacggcgc cagcgatctg ctgcgaccaa atgtcaatcc
     1861 atccgacgat ctcggacgcg ttccattttg cgagctgcgt tgcgatcgcg ttcacgtact
     1921 gagctcgtgt ggtcggatcg ttgatgacga tcagatagtt cttggcaggt gggtcaggga
     1981 gcgcccgccc agcccaccaa tagatcggat gctccttgta tccaagcgac ggcgtaaaga
     2041 cctcgagcca ctcgagcgcc gaatcagtgt ggtccagcaa ccagacgtag ccagccgtgc
     2101 cctcgtcata gatccagaaa ttgtgagaac cgccgtaggt accatcggcg tcttccacca
     2161 cggcctccgc cgcccactcg aggacggtgt tcggtacatc gatcagcgtt cgcagggtcg
     2221 cgatatccgt
//
LOCUS       DASWVF010000416         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_444399, whole genome shotgun sequence.
ACCESSION   DASWVF010000416 DASWVF010000000
VERSION     DASWVF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444399"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VGD55_07540"
     CDS             <1..1414
                     /locus_tag="VGD55_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0870236.1"
                     /translation="GMVAGGPRVIVWGWLFVGGMVMLVGAGMAEVCSSFPTAGGLYYW
                     AAKLAPDGKAPVWSWFTGWFNLLGQVAVTASIDFAMATFTGFFLNLTTGFQPTAKSLF
                     GIYTLVLIAHGILNSLGLNIIAMLNNISVWWHLTGVVVIVGALFILPDAHKHQAISSV
                     FTKYYDGLGWGFGGHAIWVVLVGLMMAQYTLTGYDASAHMTEETRDASTSGPRGIMTS
                     IWVSVVAGLVLMIGLTYAIPYAIGSDGYVAAANSGISAPGVLWVAAIGRHAAEFLILI
                     CLVAQFFCGMASVTANSRMIYAFSRDGAVPGSRFWHKVNKRTRTPTNSIWFAVVGAFI
                     LGLPSLYQNKGYSVAFFAIVSVAVVGLYVSYVIPVLLRRMRGSDFTPGPWQLGKWSPI
                     IGWTAVVWVVIICFPLMLPQFHPAGPNSWNFAPIAVLAVIGFAGIYWLVSARKWFTGP
                     KVQGTAEELAAIEAELSSLG"
     gene            1472..>2832
                     /locus_tag="VGD55_07545"
     CDS             1472..>2832
                     /locus_tag="VGD55_07545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY0870237.1"
                     /translation="MPERQGLISIEELRIEVDAGTIDTVLICLPDMQGRLQGKRLTGR
                     FFLDEVLDHDTEGCNYLLAVDVEMNTVGGYAMSSWESGYGDFVMRPDLSTLRRVPWHE
                     ATVLLMCDLIDSASVPVVAAPRNILRTQTDRLQNNGWRAMIGTELEFLLFRDTFEEAF
                     SRRYQNMTPANQYNIDYSILGTSRVEPILRRIRNGMDGAGMRVESAKGECNLGQQEIA
                     FVYDEALITCDNHTIYKTGAKEIAAQEGMSLTFMAKYDEREGSSCHIHLSLRDAGGES
                     VFAGDGVGDRHGFSRVFEQFLAGQLAAMRELTLFYAPNINSYKRFAEGSFAPTAIAWG
                     HDNRTCALRIVGHSPSSLRLENRVPGADTNPYLAVSAMIAAGLYGIEHELVLPPAFTG
                     NASVADFERLPTTLREALGLWEKSGLARSAFGDEVVAHYANNARVELAAFERSVTDWE
                     RVRG"
BASE COUNT          478 a          883 c          905 g          566 t
ORIGIN      
        1 cggcatggtc gctggtggcc cgcgggtcat cgtctggggc tggctgttcg tcggcggaat
       61 ggtcatgctc gtcggcgctg gcatggccga ggtgtgctcg agcttcccga ccgcgggtgg
      121 gctttactac tgggccgcga agctggcgcc ggacggcaag gctccggtgt ggagttggtt
      181 caccggctgg ttcaacctgc tcggccaggt cgcggtcacc gcgtcgatcg acttcgccat
      241 ggcgacgttc accgggttct tcttgaacct gacaaccggg ttccagccga ccgcgaagag
      301 cctgtttgga atctacacgt tggtactcat cgcgcacgga atcctgaact cactgggcct
      361 gaacatcatc gccatgctga acaacatcag cgtgtggtgg cacctgaccg gcgtcgtcgt
      421 catcgtcggc gcactgttca tcctgcctga cgcccacaag caccaggcaa tttcgagcgt
      481 cttcaccaag tactacgacg ggctcggttg gggtttcggt ggccacgcga tctgggtggt
      541 tctcgtcggt ctcatgatgg cccagtacac gctgaccggg tacgacgctt ctgcccacat
      601 gacagaagaa acccgtgatg cttcgactag cggcccgcgt ggcatcatga cgtcgatctg
      661 ggtgtcagtt gtggccggtc tcgtcttgat gatcgggctc acgtacgcga ttccgtatgc
      721 catcggtagc gacggctacg tggccgccgc gaactcaggc atcagtgcac ccggcgtctt
      781 gtgggttgcc gcgattggtc ggcacgccgc cgagttcctc attctaattt gcctcgtcgc
      841 gcagttcttc tgtggtatgg catcggtgac tgcgaactca cgcatgatct acgccttctc
      901 gcgcgacggc gctgtgcccg gctcgcggtt ctggcacaag gtcaacaagc gcacccgcac
      961 cccaacgaac tccatctggt tcgccgtggt gggcgccttc atcctcggcc tgccgtcgct
     1021 gtaccagaac aaggggtact cggtcgcgtt cttcgcgatc gtgtccgtcg cggtcgtcgg
     1081 tctctacgtc tcgtatgtca ttccggtgtt gcttcgccgg atgcgcgggt ccgacttcac
     1141 ccctggcccg tggcagcttg gcaagtggag cccgatcatc gggtggaccg ctgtcgtgtg
     1201 ggtcgtcatc atctgcttcc cgctgatgct gccgcagttc cacccggctg gccccaacag
     1261 ctggaacttc gcaccgattg ccgtccttgc ggtcatcggg ttcgccggca tctactggct
     1321 ggtgtctgcc cgcaagtggt tcaccggccc gaaggttcag ggtactgcgg aagaactagc
     1381 tgccatcgag gccgagctgt cgtctctcgg atagtcaggt cggtcgagag gtcgggcagg
     1441 tggtgggcgt aggaagggta ggggtgcgca gatgccggag cgccagggct tgatcagcat
     1501 cgaggaactg cgcatcgagg tcgacgccgg aacgatcgac actgtcctga tctgtttgcc
     1561 cgacatgcag ggccgcctgc aaggcaagcg actcacgggt cggttcttcc tcgacgaggt
     1621 actcgatcac gacaccgagg gttgcaacta cctgctggcc gtcgacgtcg agatgaacac
     1681 cgtcggcggc tacgcgatgt cgtcgtggga atccggctac ggcgacttcg tgatgcgccc
     1741 ggatctctcg acgctgcgtc gggtgccgtg gcatgaggca actgttctgc tgatgtgtga
     1801 cctgatcgac agcgcgagcg ttcccgtcgt cgccgcgccg cgcaacatct tgcgcaccca
     1861 gaccgaccgc ctgcaaaaca acggctggcg cgcgatgatc ggcaccgagc ttgaattctt
     1921 gctgttccgc gacaccttcg aagaggcgtt ctcgcggcgc taccagaaca tgacgccggc
     1981 caatcagtac aacatcgatt actccattct cggcaccagc cgggtcgagc caatactgcg
     2041 acgaatccgc aatggcatgg acggcgcggg gatgcgcgtc gagtcagcca aaggcgaatg
     2101 caacctgggg caacaagaaa ttgccttcgt gtacgacgag gcgctcatca cctgcgacaa
     2161 ccacacgatc tacaagaccg gagcgaagga gatcgccgcg caggagggca tgagcctcac
     2221 cttcatggcg aagtacgacg agcgcgaagg aagctcgtgc cacatccacc tgtcgctgcg
     2281 cgacgctggg ggcgagtcgg tcttcgcggg tgatggcgtg ggcgatcggc acggtttctc
     2341 ccgcgtgttc gagcaattcc ttgctggcca gctggctgcc atgcgcgagc tcacgttgtt
     2401 ctacgcgccg aacatcaaca gctacaagag gtttgccgag ggcagctttg cgccgaccgc
     2461 gatcgcgtgg ggccacgaca atcgcacctg cgcgttgcgg atcgtcggcc actccccgtc
     2521 gtcccttcgg ctggaaaacc gggtgccggg cgccgacacg aatccctacc tcgcggtttc
     2581 ggccatgatc gccgccggct tatacgggat cgagcatgaa ctcgtgctgc caccggcatt
     2641 caccgggaac gcctctgtag ccgacttcga gcgactgccg acgaccctgc gcgaagcttt
     2701 ggggctgtgg gaaaagagcg gcctcgcgcg gtcggcgttc ggcgatgagg tcgtcgcgca
     2761 ctacgccaac aacgcacgcg tagaacttgc agcgttcgaa cggtcggtga ccgactggga
     2821 gcgcgtacga gg
//
LOCUS       DASWVF010000417         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_291373, whole genome shotgun sequence.
ACCESSION   DASWVF010000417 DASWVF010000000
VERSION     DASWVF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291373"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGD55_07550"
     CDS             complement(<1..758)
                     /locus_tag="VGD55_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0870238.1"
                     /translation="MTAHQQPGERLGARGPLLTKDVSRLFERLRRTSRFALAVLAFFG
                     LVMPTANAATVVAQAAPTVVAVSVTGNTHVPTDRILAVISTKVGDPFDPAKVQADLRA
                     IADLGFFADQAPPVIRQRPDGVAITYRVIENPVVTSIRFTGNRSVSADTLLALMDTAP
                     GQVFNIKTYQQDVLKINSYYDKIGFGGQLPSHVTDVNITPEGVLTLGIQEGLTVRNII
                     ITSPPDADPILPPSVIIKALVTKPGSPYSEEQRD"
     gene            complement(859..>1999)
                     /locus_tag="VGD55_07555"
     CDS             complement(859..>1999)
                     /locus_tag="VGD55_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0870239.1"
                     /translation="DLTAHNASIDSPQYVRGTVDGAMKIASAAAMPIVSGNLTLSNAS
                     IPFAAVYRNAGGTPGDVPLPVGFDLTLIAGRNVRVQSSIIDVGATGQVVLQGTLAAPK
                     LDGVLSATPGGIFSTYNRVFRIQEAVVRFNPADGVVPYVDLRAYAHVVNPDPDPTRNA
                     VGSADITVSVRGPADELAQGTGISFSSNPPYSNEQILGLLLDASVFGAVNFGQQQNGT
                     TLPGAPGESNALNPPGTTTNQTGIINFNQEAFSLINGQLTQRFLAPMERVLGGALNLT
                     DLELTVDYGGGLGYTMLKQIGHRDVYASFGQTLTYPERTTAGFTSRPNATTSIQFNYF
                     TANGDPQITTNANGTQPFSYVQRLHGLQPLSGRQGFTFSVVRKYP"
BASE COUNT          329 a          599 c          707 g          364 t
ORIGIN      
        1 ttatcgcgct gctcttcgga atacggactg ccgggcttcg taaccagtgc cttgatgatg
       61 acgctgggcg gcagaatcgg gtccgcgtcg ggcggcgatg taataatgat gttgcggacg
      121 gtcaggccct cttgaatccc cagcgtcagc acgccctccg gcgtgatgtt gacgtcggtg
      181 acgtgcgagg gcaattgccc gccgaagccg atcttgtcgt agtagctgtt aatcttcagg
      241 acgtcttgct gataggtctt gatgttgaaa acttggcccg gtgccgtgtc catcagggcg
      301 agcagcgtgt cggccgagac gctgcgattc ccggtaaacc ggatcgatgt caccaccggg
      361 ttctcgatca cgcggtaggt gatcgcgacc ccgtccggcc gctggcgaat caccggcggc
      421 gcctggtcgg cgaaaaagcc caggtccgca atcgcgcgca ggtcggcttg caccttggcc
      481 ggatcgaagg gatcgcccac tttggtggag atcacggcca ggatccgatc ggtggggacg
      541 tgcgtgttcc cggtgaccga gacggcaacg accgtcggtg cggcctgtgc gacgacggtc
      601 gcggcgttcg ccgtcggcat cacgagaccg aaaaacgcga gcacggcgag cgcgaagcgc
      661 gacgtgcggc ggagccgttc gaacaagcgg gagacgtcct tcgtgaggag gggaccccgg
      721 gcgccgaggc gttcgccggg ttgctgatga gcggtcaacg cgccgatggt tgtgcgcgtt
      781 cccggtcccc agggtacgac agagtacgcc gagggccttg tgcgcgcaag cggccgtccg
      841 accgcgcgag gcaagcgctc acggatactt ccgcaccacc gagaaggtga atccttgccg
      901 tcctgagagc ggctgcagac cgtgcaaccg ctgcacgtag ctgaagggct gggtaccgtt
      961 ggcattggtc gtgatctgcg gatcgccgtt ggcggtgaag tagttgaact gaatggacgt
     1021 cgtggcgttg ggccgtgagg tgaagcccgc cgtcgtgcgc tccgggtagg tcagcgtctg
     1081 cccgaagctc gcgtagacgt cgcgatgccc gatctgcttg agcatcgtgt agccgaggcc
     1141 gccgccgtag tcgacggtga gttcgagatc ggtcaggttg agcgcgccgc cgagcacgcg
     1201 ttccatcggt gccaggaagc gctgcgtgag ctgcccgttg atcagcgaaa aggcttcctg
     1261 gttgaagttg atgatgccgg tttggttggt cgtcgtaccc ggcgggttga gggcgttcga
     1321 ttcgcccggt gcgcccggca gcgtcgtgcc gttctgctgc tgtccgaagt tgaccgcgcc
     1381 gaacaccgac gcgtcgagca gcaggccgag gatctgctca ttggagtacg gcgggttcga
     1441 gctgaaactg atcccggttc cctgcgcgag ttcgtcggcg ggaccccgca cgctcaccgt
     1501 gatgtcggcg ctcccgacgg cattgcgcgt cggatccgga tcgggattga cgacgtgcgc
     1561 gtaggcacgc aggtcgacgt acggtacgac gccgtcggcc ggattgaaac gcacgaccgc
     1621 ttcctgaatg cggaagacgc gattgtaggt ggagaagatg ccgcccggcg ttgccgacag
     1681 cacgccgtcg agcttcggtg cagcgagcgt tccttgcagg acgacctggc cggtcgcgcc
     1741 gacatcgatg attgacgact gcacgcgcac gttgcgcccg gcgatcagcg tcaggtcgaa
     1801 gccaaccggc agcggcacgt cgcccggggt accgcccgcg ttgcggtaga ccgccgcgaa
     1861 agggatcgac gcattcgaga gggtcaggtt ccccgacacg atgggcatcg cggccgcgct
     1921 cgcgatcttc atcgcaccat cgaccgtccc gcgcacgtac tgtggcgagt cgatcgatgc
     1981 gttgtgtgcc gtcaggtcg
//
LOCUS       DASWVF010000418         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163776, whole genome shotgun sequence.
ACCESSION   DASWVF010000418 DASWVF010000000
VERSION     DASWVF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163776"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2471
                     /locus_tag="VGD55_07560"
     CDS             156..2471
                     /locus_tag="VGD55_07560"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY0870240.1"
                     /translation="MVQWRSLLPPDRPPPLFRWAKYLLVIGVAIFAASTFVVPLTSSA
                     KPIFDGWLYDSLTVLGAIVVAGRAWSVATERLAWGLLAVAIAANAVGDVVYTLFVGDN
                     GPFPSIAEPFYLAFYPCAYIALLLLIRARATRMAAGVAIDGIACALAMAAVAATIAFG
                     PILASTGGSVAAVAVGLAYPIADLLLLALSVAMLVILGWRSERRVLLLALAFAIYAVA
                     DTVYLYQVAKGSYVEGRFIDAFWPVGTLLTAYASWMPTRRLADRRLDGIRALAAPFGC
                     TAAAVAILVTGTQMKVPDLAVALAAGTLLAVTARLAITFRDVISLAETHTQALTDELT
                     GLANRRSLVTALGAHAAHRTRIDRDGESSLALMLIDLDRFKEINDSLGHDVGDELLRQ
                     LARRLERYIRPGDLLSRFGGDEFAILLSPERDQTAAERVAARIAAALDEPFALDDLTL
                     HVNGSIGIALYPHHCSDPMQLLQRADVAVTVAKKTPTRVALYQIGEDPHSKERVQLIE
                     ALRVGIARGELICHYQPKVSAATGATDSVEALVRWQHPERGLLAPDAFLPLAEQAGLM
                     RPLTLAVLEMALRDASRWRWDNHVVSVAVNLSVTNLLDVELASDVAALLDEHQLPAST
                     LILEITETVLMTDSTRAKSVVDALHALGVGLSVDDYGTGYSSLAYLQDLAIDELKLDR
                     SFIMRLSEDPRTAAIVRSTVELAHSLGLRIVAEGVEDAGTVEMLHRYGCDVIQGYFYS
                     RPIPAAQLTTWLAKGPAAEPSRHLFSRAHPA"
     gene            complement(2570..>3920)
                     /locus_tag="VGD55_07565"
     CDS             complement(2570..>3920)
                     /locus_tag="VGD55_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY0870241.1"
                     /translation="SRTFILMTNGLAAPADDITADVVGPACDAADDVAMLAQLAAMEP
                     GPVAMMVLSGIDPGTLDDDGRLDYAREWDRQQRWASARTQSALAEIMVCDLPDDDAEI
                     SETLEYRAAMVATALQWSPRGAMGRLDLAMRIVYELTGTFALLNAGQLSLRHATVMAE
                     ETESLDQISIALVEARVLERGVGQTPAEFGRSVRKAVLAVVPLVAQLQHASALAGRNV
                     RRAWGRDGMATLVATLPAADAEIVFLALDAVARKAAAADGDTTIGIDARRADALVAWA
                     LAALDDPDLPKQHGRPVALRLTMDLPSLLGMADNPAELSHYGMIPASAGRILAADAPW
                     QRFITEPVTGHLLDFGSTVYRPPQALRDFLIARDRVCRFPGCSMAAERCDLDHCCPFD
                     KGGHTSACNCHALCRRHHIAKTTGGWLLRCNGKGGVTWVAPTGQQFFVAAENHDPAL"
BASE COUNT          671 a         1319 c         1249 g          681 t
ORIGIN      
        1 gtcttgctgc tggcaacctg gggagccttg ctcgtcgcga cctgaaggcg tctgacggcc
       61 tgaacccaga atcgctgagt gggtacgaaa tacggtcaga tcggactcgc gccggtcgat
      121 ctagaggtat ggcgcctgag cggacgcgag gggcgatggt gcaatggcgc tcgctgctgc
      181 cccctgatcg cccgccgccg ctgtttcgtt gggccaaata cctgctcgtg atcggcgtgg
      241 cgatattcgc cgcgtcgacc ttcgtggtgc ccctcacttc gagcgccaag ccgatcttcg
      301 acggctggct gtacgactcc ctgacggtgc ttggcgcgat cgtggtcgcc ggccgcgctt
      361 ggtcagtcgc aaccgagcgc ctcgcctggg gcctactcgc ggtggcgatt gcggccaacg
      421 ccgtgggcga cgtcgtctac acactgttcg tcggcgacaa tggaccgttc ccttcgatcg
      481 ccgagccgtt ctacctcgcc ttctacccgt gcgcgtacat cgccctgctc ctgctgattc
      541 gggcgagagc aacccggatg gctgctggcg tcgcgatcga cggcatcgcg tgcgcgctcg
      601 ccatggcggc agtcgctgcg acgatcgcct tcggcccgat ccttgcctca actggcggct
      661 ccgtcgccgc agtcgcggtg gggctcgcgt atcccatcgc cgaccttctg ctgctcgcgc
      721 tttcggtcgc aatgctcgtg atcctcggct ggcgcagcga acggcgggtg ctgctgctcg
      781 ccctcgcgtt cgctatctac gcggtagccg acacggtgta cctctaccaa gtcgcgaaag
      841 gcagctacgt cgagggcagg ttcatcgacg cgttctggcc ggtgggcacc ttgctgacgg
      901 cgtacgcgag ttggatgccc acccggcgac ttgccgaccg acgactcgat ggcatccgcg
      961 cacttgccgc accgtttgga tgcaccgcgg ctgcggttgc gattctcgtg accggaacgc
     1021 agatgaaggt cccagatctc gcagtcgcgc tcgccgcggg cacattgctg gctgtcaccg
     1081 cccggctggc gatcaccttc cgcgacgtca tcagcctcgc cgagacccac acgcaagcgc
     1141 tcaccgacga gctcaccggc ctcgcgaacc gtcgctcgct cgtcaccgcg cttggcgcgc
     1201 acgctgcgca taggacgcgc atcgaccgcg atggcgagtc gagcctcgcc ttgatgctca
     1261 tcgacctgga tcgcttcaag gagatcaacg attcgttggg ccatgacgtc ggcgatgaac
     1321 tgctccgaca gctcgcgagg cggctcgagc gctacatcag acccggtgac ctgctgagca
     1381 ggttcggtgg cgacgagttc gcgatcctgc tgtcaccaga acgggatcag acggcggcgg
     1441 aacgggtcgc ggcaaggatc gctgcagcgc tcgacgaacc gttcgcgctc gatgacctga
     1501 cgttgcacgt caacggcagc atcggcattg cgctgtaccc acatcactgc tccgatccga
     1561 tgcagttgct tcaacgcgcg gacgtcgcgg tgaccgtcgc caagaaaacg ccgacccggg
     1621 ttgcgctgta ccagataggc gaagacccgc acagcaagga acgggtacag ctcatcgagg
     1681 cgctgcgagt tggaatcgca cgcggtgaac tgatctgtca ctaccagccg aaagtcagcg
     1741 ctgcgaccgg cgcgaccgac agcgtggaag cgctggtgcg ttggcagcat cccgaacgcg
     1801 gactcctggc gccagacgca ttcctgccgc tggccgagca ggccggactt atgcggccgc
     1861 tcaccctcgc ggtgctcgag atggccttgc gggacgcgag tcgctggcgt tgggacaacc
     1921 atgtcgtgtc tgtggccgtg aacctgtcgg tgacgaacct gctcgacgtc gagctcgcaa
     1981 gcgacgtcgc cgcactgttg gacgaacatc agcttccggc gtccaccctg attcttgaaa
     2041 tcaccgagac agtgctgatg acggactcaa cgcgagccaa gtcagtggtc gacgcgctgc
     2101 acgcactggg tgtggggttg tcggtcgacg actatggcac ggggtactcg agccttgcgt
     2161 atctgcaaga cctcgcgatc gacgaactca agctcgaccg cagctttatc atgcgactgt
     2221 ctgaggaccc gcgcaccgca gcgatcgtcc gatcaaccgt cgagcttgcc cacagtctcg
     2281 gcctgcgcat cgtcgcggag ggcgtcgagg acgccgggac cgtcgaaatg ctccacagat
     2341 acggctgcga cgtgatccag ggttacttct acagccggcc gataccagcc gcgcagctaa
     2401 caacctggct cgcgaaggga cccgccgccg aaccatcacg gcatctcttc tcgcgcgccc
     2461 accccgccta gggcaagctc ggccgccccg catagggcaa gctcggccgc cccgcctagg
     2521 gcaaactcgg ccgcttatcg cgctcacctc gatttgggca ggctccgccc tagagcgctg
     2581 ggtcgtgatt ctctgcagcc acgaagaact gctggccggt tggcgcaacc cacgtcacgc
     2641 cgcccttgcc gttacatcgg agtaaccaac cgccggtcgt tttcgcgatg tgatgtcgtc
     2701 gacacagtgc atggcagttg cacgcagatg tgtgaccgcc cttgtcgaac ggacaacagt
     2761 gatcgagatc acatcgctcg gctgccatcg aacatccggg gaaccggcag acccgatcac
     2821 gtgcgatcag gaagtctcga agcgcttggg gcggcctgta gaccgtgctg ccgaagtcga
     2881 ggaggtgccc ggtgacaggc tcagtgatga agcgctgcca cggcgcgtca gctgcgagga
     2941 tgcggcccgc cgatgccggg atcatgccgt aatggctcag ctcggccggg ttgtcagcca
     3001 tgccgagcag gctcggcaag tccatcgtga ggcgaagcgc aaccgggcgg ccatgctgct
     3061 tcggcaaatc cggatcgtcg agtgcggcca gcgcccacgc cacgagcgca tcggcgcggc
     3121 gggcgtcgat gccgattgtg gtgtcaccgt ctgccgcagc agccttgcgc gcgacggcgt
     3181 cgagcgcgag gaagacgatc tcggcatcgg ctgcaggcag agtggccacc agggttgcca
     3241 tgccatcgcg accccaggcc cgccggacgt tgcggccggc cagcgccgaa gcgtgctgaa
     3301 gctgtgcgac cagcggcacc acagccagaa cagccttccg gacactgcgc ccgaactccg
     3361 cgggcgtctg cccgaccccg cgctccagca cgcgtgcttc gaccaaagcg atcgagatct
     3421 ggtcaaggct ttcagtctcc tcggccatca cggtcgcatg ccgcagactc agctggccgg
     3481 cattcagcag tgcgaaggta cccgtcagtt cgtagacaat gcgcatggca agatcgagac
     3541 ggcccatcgc gcctcgcggc gaccattgca acgcggttgc gaccatcgcg gcgcggtact
     3601 cgagagtctc gctgatctcc gcgtcgtcat cgggtaggtc gcacaccatg atctcggcga
     3661 gagcactctg cgtgcgcgct gacgcccaac gctgctgccg atcccactct cgcgcgtaat
     3721 cgaggcgacc gtcgtcgtca agcgttccgg gatcgatgcc agagagcacc atcatcgcca
     3781 ccgggccagg ctccatcgcc gcgagctgag cgagcatcgc cacatcgtca gcggcatcgc
     3841 acgctggacc gacgacatca gcagtgatgt catcggccgg tgcggcgagc ccgttggtca
     3901 tgagaatgaa cgtacgtgag
//
LOCUS       DASWVF010000419         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_36574, whole genome shotgun sequence.
ACCESSION   DASWVF010000419 DASWVF010000000
VERSION     DASWVF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36574"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VGD55_07570"
     CDS             complement(<1..1720)
                     /locus_tag="VGD55_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEY0870242.1"
                     /translation="MALRIALVLFVLLIAAAAWVAYNGMRAKSDLLRAADDVAVLQHQ
                     AESGDVAGERTSLANLQREAHAARTETRGPIWALARSTPLVGGNASAVATVASAVDDL
                     AQHALPSLVGASETLDLQQIAPHDGRIDLAPIQHAAPAILGAASAVQRVRDQVAGIST
                     SGLLPVVRTSVLRLQGDLDKAVTTTSNAARAVTLLPPMLGADGARTYALLFLNNAEVR
                     ATGGIAGSWAIVHVENGGVAIVRQGSAGDVNNHLTGVPVPPDVAAIYTNRPGTFFQDV
                     DMSPSFPTDAALASEMFHQAYGIQVDGVISTDPVALAGLLSATGPVKLPLGGSLTASN
                     AVSLLLSQVYANIQNPAQEDAYFAQAAYAVFAALSAGQGDARATIKSLADAAGAGRLT
                     VWSQHPAEENLLAGTALEGAMPSNEPAGNPTVGVFLNDGTGAKLDYYLREDVAVAPAA
                     SCPARRIAFHVRVTLTSTVPADAAGLPAYVKGGASAGTMRSQVVFAAPIGGSIVSASV
                     DGVRTSIGSGVESGRSEGVVFVDLAPGAEKTIDVELVTASLSSKLRTRGLSPQVRSTP
                     LATPALL"
     gene            complement(1798..2427)
                     /locus_tag="VGD55_07575"
     CDS             complement(1798..2427)
                     /locus_tag="VGD55_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870243.1"
                     /translation="MTSDEPAAIIAVCTGNLCRSPVVERLFRLGFSTRWPSAALPLPV
                     ASAGTQAALGHQIPGSLTSRLLTVGADDSPFVPRQVTPEMLDAAELVIALTRAHRAAV
                     VELHPRAVHYTFTLRELARLATSLPPGDRAPIEPLARLRVFVTAAAGARGASRPGDPT
                     EDDVPDPYGGNDARYGKAFDSLHEATSSILTALCPFQGSSVGVTVLPSR"
     gene            2527..3495
                     /locus_tag="VGD55_07580"
     CDS             2527..3495
                     /locus_tag="VGD55_07580"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0870244.1"
                     /translation="MPAAATNQVTVAVGDEELLVERAIAAVVARARAADAETDVRDLL
                     ATSLEPGQLAELTSPSLFGERRVLVLRDVEQLAKEMVDEVSAALRQADDELGIVLTHK
                     GGARGKQLIDAALAEGATRIDCAKITRPGERIDFVRAEFRVAGRTISESGARALIDTV
                     GTELRELAAACAQLCTDTDGQVDSEDVARYYRGRAEANGFAVSDRALEGRLGDALEQL
                     RWALAVGTAPVLITSALAQGIRALAKVASAPRGLRGADLARDVEMPPWKVERVQRQLP
                     GWTPVGVAAAVTAIARADEQVKGGGTDAGYALEQAVLAIVAARGAA"
     gene            complement(3503..>3599)
                     /gene="rpsT"
                     /locus_tag="VGD55_07585"
     CDS             complement(3503..>3599)
                     /gene="rpsT"
                     /locus_tag="VGD55_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0870245.1"
                     /translation="RQLDKAASKGVIHANQAANRKSALAKRAATL"
BASE COUNT          580 a         1209 c         1253 g          557 t
ORIGIN      
        1 ggagtaacgc tggcgtcgcc aagggagtcg accgcacctg cggcgacaac ccacgtgtcc
       61 gaagtttcga cgacagcgac gcggtgacga gttcgacatc gatcgtcttt tccgcgcctg
      121 gcgcaagatc gacgaacacg actccctccg agcgacccga ctccacccca ctgccgatcg
      181 atgtgcgcac gccgtcgacc gatgccgaaa cgatcgagcc gcctatcggc gcagcgaaaa
      241 ccacctgcga ccgcatcgtt cccgccgacg cgcctccctt cacgtacgca ggcaggcccg
      301 cggcatcggc cggaaccgtc gaggtcagag tgactcgcac atgaaacgcg atccgtctag
      361 caggacatga ggccgcggga gcgacggcca catcttcgcg caagtagtag tcgagcttcg
      421 cgccggttcc gtcgttcaga aacacaccga ccgtcgggtt accagcaggc tcgttagagg
      481 gcatcgcgcc ctcaagtgcc gtgcctgcga gcaggttctc ctcagcggga tgctggctcc
      541 acacggtgag acggcctgct cccgcggcgt ccgccaaaga cttgattgtc gcccgcgcat
      601 cgccttggcc ggccgacaac gcagcgaaga cggcgtatgc ggcctgcgcg aagtaggcgt
      661 cctcctgcgc tgggttctgg atgttcgcgt agacctgcga cagcaacagc gacaccgcgt
      721 tgctagcggt cagcgatcca ccgagtggca gcttcaccgg tccggtcgcg ctgagcagtc
      781 cggctagcgc caccgggtcg gtcgagatca cgccatcgac ctgaattccg tacgcctggt
      841 ggaacatctc gctcgcaagt gccgcatcag tcgggaacga cggtgacatg tcgacgtcct
      901 ggaagaacgt gcctggtcga ttcgtataga tcgccgcgac atcgggcggt accggcacgc
      961 cggtcaaatg attgttcacg tcgccggccg atccctgcct cacgatcgcg acaccgccgt
     1021 tctcgacgtg cacgatcgcc cacgaacccg cgatgccacc cgtggcgcgc acctcggcgt
     1081 tgttcaagaa cagcagcgca tacgtccgcg ccccgtcggc accgagcatc ggcggcagca
     1141 gcgtcactgc gcgcgccgcg ttcgacgtcg tggtgacggc tttatcgaga tcgccctgca
     1201 gtcgcaacac cgacgtacgt acaacgggca gcaggccgga cgtcgagatg cccgcgactt
     1261 ggtcacgcac acgttgcacg gccgaagcgg cgcccagtat cgccggcgcc gcatgttgaa
     1321 tcggcgcaag atcgatgcgg ccgtcgtgcg gcgcgatttg ttgcaggtca agcgtttccg
     1381 aagcaccgac caatgaggga agcgcgtgtt gggcgaggtc gtcgaccgcc gacgcgaccg
     1441 tcgcaactgc actcgcgttg ccgccgacga gcggcgtaga tcgcgcgagc gcccagatcg
     1501 gtccgcgcgt ttcggttcgc gccgcgtgcg cttcgcgctg caggttcgcc aggctggtgc
     1561 gctcgccggc cacgtcaccg ctctcggcct gatgttgcaa cactgcgaca tcgtcagcag
     1621 cgcgcagcag gtcgctcttg gcccgcatcc cgttgtaggc gacccatgcc gcagcggcaa
     1681 tcagcagcac gaacagaaca agcgcgatgc gcagggccaa gccgcggcgc ggccggcgac
     1741 gccctgcgtg tcgtggaccg actgaggtcg agagtgcggg agcactcagg tgcgttgtca
     1801 tcgcgagggc aggacggtca cgccgaccga tgagccctga aatgggcaca gtgcggtcag
     1861 gatcgacgac gttgcttcat gcaggctgtc gaacgccttg ccgtagcggg cgtcgttgcc
     1921 gccgtacgga tccggcacgt cgtcttcggt cggatcgccc ggtctcgatg ccccccgcgc
     1981 acccgccgct gccgtaacga agacccgcag cctggccagc ggttcgatcg gtgcccggtc
     2041 gcctggcggc agcgatgtgg ccagcctcgc tagctcgcgc aaggtaaagg tgtagtgcac
     2101 tgcgcgcgga tgcagctcta cgaccgcggc tcgatgcgcg cgcgtgaggg cgatgaccag
     2161 ctccgcggcg tccaacatct ctggagtgac ctgtcgcggt acgaacggcg aatcatctgc
     2221 gccgacggtc agcagtcgcg acgtgagcga cccggggatc tggtgaccca gcgcggcttg
     2281 cgtcccggcg ctcgcgaccg gcagcggcaa cgccgcgctt ggccaccgcg tgctgaagcc
     2341 caggcggaag agccgctcca ccacggggga gcggcacagg ttgccggtgc agacggcgat
     2401 gatcgccgcg ggctcgtcac tggtcatcgg ggtgattctc ggcgtttcgc accggagcct
     2461 gagcgcggcg agcagtgtcg tgcataagcc ggacgccgta acgctgcgac gtggcatgct
     2521 gccctcgtgc cagccgccgc cacgaaccag gtgacggttg ccgtgggcga cgaggaactg
     2581 cttgtcgaac gggcgatcgc cgccgttgtc gcgcgcgcca gggccgccga tgccgagacc
     2641 gacgtccgcg acctgctggc gacgtcgctc gagccggggc agctcgccga gctcaccagt
     2701 ccctctcttt tcggcgagcg tcgcgtgctt gtcctgcggg acgtcgagca gctggcgaaa
     2761 gagatggtcg acgaggtcag cgcggcgctt cgccaggccg acgacgagct ggggatcgtg
     2821 ctcacgcaca agggcggcgc ccggggcaag cagctgatcg acgccgcgct ggccgaaggt
     2881 gcgacccgga tcgactgcgc gaagatcacc agacccggcg agcggatcga cttcgtccgc
     2941 gccgagttcc gcgtggctgg ccggactatc tccgagagtg gcgcccgcgc gctgatcgac
     3001 acggtcggta ccgagctgcg cgagctcgcg gccgcctgcg cgcagttgtg cacagacacc
     3061 gacgggcagg tcgactccga ggatgttgca cgctattacc ggggtcgggc agaggcgaac
     3121 ggcttcgcgg tctccgaccg cgcactcgag ggtcggctcg gcgacgcgct cgagcagctg
     3181 cggtgggcat tggcggtggg tacggcgccg gtcttgatca ccagcgcgct cgcgcagggg
     3241 atccgggcgc tggcgaaggt ggcgagcgcc cctcgcggac tgcgcggggc cgacttggcg
     3301 cgcgacgtgg agatgccacc gtggaaggtc gagcgggtgc agcgtcagct tcccggctgg
     3361 accccggtcg gcgtcgcggc cgcggtcacc gcaattgcga gggccgatga gcaggtcaaa
     3421 gggggcggga cggacgccgg ctacgccttg gagcaggccg tgctggcgat cgtggccgcg
     3481 cgcggcgcgg cctgacgaat tcttagagtg tggccgcgcg cttggccagc gctgacttgc
     3541 ggttcgcggc ctggttggcg tggatgacgc ccttgctggc ggccttgtcg agctgacgg
//
LOCUS       DASWVF010000420         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_11327, whole genome shotgun sequence.
ACCESSION   DASWVF010000420 DASWVF010000000
VERSION     DASWVF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11327"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGD55_07590"
     CDS             <1..220
                     /locus_tag="VGD55_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870246.1"
                     /translation="WVFVYIGTTPLGSVLTGWICSTAGPRVALLVGAGACVVVAGVAA
                     RVHTPPDPDAALTDLQEMASQRPMPPGR"
     gene            complement(236..>2524)
                     /locus_tag="VGD55_07595"
     CDS             complement(236..>2524)
                     /locus_tag="VGD55_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0870247.1"
                     /translation="GCRHQPLSRAGHPFSHERNTVNIAGRVGSWSARHRKTAIIGWLL
                     LVVAAVVIGSAGTKQITGAEGSNGQSAKAERVLLASGLPDTASENVLVQTKSGLPDAA
                     ALRTAVSQVVTAVGKTNLTANLHSPLDPDGQQYVSHDGHSMLVSFDMTGASDTAVSRV
                     QPLLDAVAGVQRANPSLRVEEVGDASSEKAFNATIGKDFKRAELFSIPLTLGILLAVF
                     GALVAAVVPIGLALTAFIVAGGLLALTSRAVHIDGTATSVMLLVGLAVGVDYTLFYLK
                     REREERALGRSPAEALRIAAATSGRSILVSGLTVVAAVGGLFLTGNATFKGVAEATVL
                     VVAVAVLGSVTVLPALMSLLGDRIEKGRLPFIRSARSGDSRAWRAVLRFVLRRPGRSA
                     VVASGVLVALALPLVGMKTALPGSTDLPKTLPFVQAYDRLSTAFPGGPQPAYVVVAAH
                     DVNAPAVQAGLRAMSAQAVASGEMSAALSARVNAAGTVASVAIPLNGNGSDAASNQAL
                     KTLRDKVIPETIGAVPGVRAYVTGQTAGSKDFGHQLATHAPYVFVFVLGLAFVLLLGA
                     FRSLTVALTAIALNLLSIGAAYGVMVLVFQHHWFDGILGYTSTGSIATWIPLFMFAIL
                     FGLSMDYHVFIVSRIVENRRRGLTTRDAVERGIASTAGVVTSAAVIMVAVFSVFGTLS
                     QVSMKQIGVGLAAAVLLDATIVRAVLLPAVMAMLGERNWYLPRWLQWLPAGSEASDRA
                     EPARPDAVLEPAEGQHTTVAIR"
BASE COUNT          470 a          880 c          788 g          386 t
ORIGIN      
        1 ctgggtgttc gtctatatcg gcacgactcc gcttggcagt gtgctcactg gctggatctg
       61 ctcgacagcg ggcccacgcg ttgcgctgct cgtcggcgcc ggcgcgtgtg ttgtcgtcgc
      121 gggtgtagcg gcccgcgtgc acaccccgcc ggaccccgac gctgccctca ccgacctcca
      181 agagatggcg agtcaacgcc cgatgccgcc agggcgttga ctcgcgagac catctctatc
      241 gaatcgcgac ggtggtgtgt tgcccttcag caggttccag cactgcgtct ggcctcgcag
      301 gctcagcgcg atcgctggcc tccgagcccg ctggcaacca ctgcaaccac cgcggcaggt
      361 accagttgcg ttcgccgagc atcgccatca cggcgggcag caagaccgcc cgcacgatgg
      421 ttgcgtcgag taggacggcg gccgcgagac cgacgccgat ctgcttcatc gacacctgtg
      481 acagcgtgcc gaacaccgag aacaccgcga ccatgatgac ggcggcgctc gtcacgacgc
      541 ccgcggttga cgcgatgccg cgctccacag cgtccctggt ggtcaagcca cgccgccggt
      601 tctcgacgat gcgactcacg atgaagacgt gatagtccat cgagagtccg aacaagatgg
      661 cgaacatgaa aagcgggatc caggtcgcga tcgaacccgt cgaggtgtaa cccagaatgc
      721 cgtcgaacca gtggtgctgg aagaccaaca ccatcacgcc gtaggcagcc ccgatcgaca
      781 gcaagttcag cgcgatcgcc gtcagcgcga ccgtcaacga acggaacgcg cccagcaaca
      841 acacgaacgc cagcccaagc acgaacacga acacgtacgg cgcgtgggtt gcgagctgat
      901 ggccgaagtc cttcgagcct gcggtctggc ccgtcacgta cgcgcgaacc cctggcaccg
      961 cgccgatcgt ctcgggaatc accttgtccc gaagcgtttt caaggcctgg ttggacgccg
     1021 catcactgcc gttgccgttc aacggaatcg cgaccgatgc cacggtgcct gctgcgttga
     1081 cccgagccga cagcgccgcc gacatctccc ccgatgccac cgcctgcgca gacatggcac
     1141 gcagccctgc ctgcacggct ggtgcattca cgtcgtgagc tgccaccacc acataggcgg
     1201 gctgcgggcc accagggaac gccgtcgaca accggtcgta ggcctgcacg aaaggcagcg
     1261 tcttcggcag atcagtgctg ccaggcaatg ccgtcttcat gccgactaga ggcagcgcga
     1321 gcgccactag aacgcccgac gcgacgactg ccgagcgacc cggccggcga aggacgaagc
     1381 gcagcacggc gcgccacgcc cggctgtcac cagaacgcgc ggagcgaatg aagggcaacc
     1441 ggcctttctc gatgcggtcg ccgagcaacg acatgagcgc cgggagcacg gtgactgatc
     1501 cgagtaccgc aaccgcgacg acgagaaccg ttgcctccgc gacacccttg aacgtcgcgt
     1561 tgccggtcag gaagagcccg ccgactgctg caaccacggt gagacctgac accaggatcg
     1621 agcgacccga cgtcgccgcc gcaatccgca atgcctcggc gggcgagcgg ccgagcgcac
     1681 gctcttcacg ctcgcgcttg agatagaaca gcgtgtagtc gacaccgacc gcgagaccta
     1741 ccagcaacat gaccgacgtc gccgtaccgt cgatgtgcac cgcgcgactg gtcaacgcga
     1801 gcagtccgcc cgccacgatg aacgccgtca gcgcgagccc gatcggcacg actgccgcga
     1861 ccaaagcgcc gaacacagca agcaggattc ccagcgtcag cgggatcgag aagagctcgg
     1921 cgcgcttgaa gtccttgccg atcgttgcgt tgaacgcctt ctccgagctc gcgtcgccga
     1981 cctcctcgac gcgcaggctc gggttggccc gctggacccc tgcgacagca tcgagcagcg
     2041 gttgtacgcg cgacaccgcc gtgtcggacg cgccggtcat gtcgaacgac acgagcatcg
     2101 agtgcccgtc atgcgacacg tattgctgcc cgtctgggtc gagcggtgaa tgcaggttcg
     2161 cggtgaggtt ggtcttgccg acggcggtga ctacttggct gacggcggtc cgcagcgccg
     2221 cggcgtccgg cagaccgctt ttggtttgta cgagcacatt ctccgacgcg gtgtcaggca
     2281 gacccgacgc gagcagcact cgctccgctt ttgccgactg cccgttcgag ccctcggccc
     2341 cggtgatttg cttggtcccc gccgaaccga tcacgaccgc tgcgaccact aacagcagcc
     2401 atccgatgat ggccgtcttg cgatgacgtg cactccagct gcctacgcga ccggcgatgt
     2461 tcacggtgtt cctctcgtga ctgaacggat ggcctgccct cgagagtggc tggtggcggc
     2521 accc
//
LOCUS       DASWVF010000421         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_87571, whole genome shotgun sequence.
ACCESSION   DASWVF010000421 DASWVF010000000
VERSION     DASWVF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87571"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VGD55_07600"
     CDS             complement(<1..2004)
                     /locus_tag="VGD55_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870248.1"
                     /translation="MGAIAMCCWVITGLAVRVGFNAGNASYPVSLQDASWIQAPDHAP
                     RAWFRDQWIVPSSATAMTLWYDTPDVVAIDINGISVPLSYHGDLNAEPDVPNARPLVD
                     AVDLSAFAVQGLTTIVLDVTSQDRSAARIRAKVVVTGHNQSATEYTSSGPSSTWLATS
                     NAALLHINGLGGQPAFSASSYPTAGWSAVAAATGRAANRAMAPESVVDRPAPDVGVQA
                     SISDAVVSRTLTLGSLPGWVWLRLWTNGISWIQVNGTDINAGGIGNIDQLSGAVDSTS
                     ASRSNTIPLFQTAPHGETSYLLNIGPWLHKGANSVRIHVTGSLGGVSLLGDIVSSLGA
                     TSLADTGWRATDTLGRSATAVVTGAPALSGMPATAGTGQSTARPIVTGRIVKVRLTSD
                     LYSEPANVRWIRHTLYAALLFLGCAAAIALLTRKRRHRVLPSTALVLVSLLPSFGLVL
                     VANRAAAWSEAVPPWPYSDSLTVVTVALAAVGLILAAVTGLPHRYRTHEMAPRLALRS
                     AVARWRERYTGRTLGRLATVNTAIWLIAIASTALLAYRLSYEPLWQDELSSLAAAEGI
                     RAHILPQWPSGFLYFKSELYSAVIAVIGGLFGDGPVALRLPSVLLHGATVVLFGRGLL
                     PLVVKRNDVRIVATLVFAIAPIELLEARDVRMYQMVQFMAVAFL"
     gene            complement(2034..3404)
                     /locus_tag="VGD55_07605"
     CDS             complement(2034..3404)
                     /locus_tag="VGD55_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870249.1"
                     /translation="MSLALDPTIAHAHVPQQRQHSVPKASRKERLQLFAFLAGIGGTV
                     VALELPWFDRHTFVDIGPASLRFAVGAGWSGWGMTWGSVAWLLVAMCCLCRFSSLSPL
                     KDTRAGRFAATQTRTVAWCIASLPVVVIVYERYVAYATIQSLLYDQARYIQFANELPY
                     NVGGTPLVSVFGISMTPQWSLLLNAVGAGLPIFALAGMSVVLTTSRSVRTFDRSTAPG
                     SKNSRTFIMSLVVIVAALSLFAMPLLARWEQYQGSQAATAGKYAPASRNFQRALVAQP
                     GLASSNSWVNTEAQIEFAGNGPSSAGGELWAAQTYADAGYPVQQLAALETAYTLDPTS
                     VVISEDLVLASVGQAVARTDPTPLLGLPAALQNDDLVRYTLGRIRYRLRDDVDARASL
                     LTVAATARSGDMRSSAWTYLAFIDEDSRHLLAARGDIERALALDPDHANTQAAAYATG
                     LYELAR"
     gene            complement(3407..4405)
                     /locus_tag="VGD55_07610"
     CDS             complement(3407..4405)
                     /locus_tag="VGD55_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0870250.1"
                     /translation="MALVLVIVVGSLTTIAVALRLKSSPTVVVATKAAPGFVQVTANG
                     TGALGAIKDATVPVSFPGVSDPIIVKSVSVVVGTQVRAGDPIATIDPTAIQDYVDQLQ
                     AVVNNDKVAVSVAATAVQKAVLQATLEVEEQHLAAALQYSSTIVAPTSGVISNLFGNV
                     GSVILSGRPIAEIISLSELRATIAVPATQVGTISVGEGVALTFPQLPSVTATASVQAI
                     SPGSSNQGLNVPVTIVLNTVSPLLRVGMQVYATFSKQVAATTALPLIAVHDPDGSAFV
                     FTVDADSRVHEQPVVVAGHDLSTAALASGLPAGALVVMTNAQHLKDGDLVKVSKVQ"
     gene            complement(4522..>6004)
                     /locus_tag="VGD55_07615"
     CDS             complement(4522..>6004)
                     /locus_tag="VGD55_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0870251.1"
                     /translation="GVRVTVDEELLRPVEFRIGARAAGLSKSGGRHSQTYPRLIASDG
                     TELEPRTMRPGSRAFRFAVVCWVGALFFVLAAARPIIQGHGSGTTSQVTLDLLSVFWA
                     IQFVSAAIGLIGLLWPRQQPHTPVSTLAHEMNGRLVSFRIVTRGQNLDALRWSIRSVQ
                     AEMSALPVFEYVIELATDRIVDDIDPSVVQLVTPTEYQTPGGARYKARALHYALTASA
                     IPSDAWIFHLDEESHITTSVIMGIAEAIVEEERTGQLRVGQGAILYHRDFRRHPLLTL
                     ADTLRTGEDLGRYYLQQRIGRAVFGLHGSFILARNDVEGAVGFDLGPQGSITEDAWWA
                     LLEMDRGVRTRWIDGFVVEQSTQSAGDFVKQRRRWFLGLVRVVLHAPVAAQHRFWLAL
                     LVATWAAMPLSIFGVLFQLWTDARPPTALFYAGVASFAIFATHYCVGLQLNLQHHRVR
                     WGWRRLCLYVGQLLLLPAFALLETAALVYAFMEPGYDFHVVRK"
BASE COUNT         1198 a         1831 c         1918 g         1057 t
ORIGIN      
        1 aaggaacgcc actgccatga actggaccat ttggtacatg cggacgtctc gagcttctag
       61 cagttcgatc ggtgcgatag cgaagacgag cgtcgcgacg atccgcacgt cattgcgctt
      121 gacgacgaga ggcagcagac ctcgaccgaa caacacaacg gtcgcgccgt gcagcaatac
      181 cgaggggagc cgaagcgcca ccggcccgtc tccgaacaac ccaccgatga ccgcaataac
      241 cgcgctgtag agctccgact tgaagtagag aaagcccgac ggccattggg gaaggatgtg
      301 ggcgcggata ccctccgcgg ccgcgaggct cgacagttcg tcctgccaga gaggctcgta
      361 cgataagcgg taggcgagca gggccgtgct tgctatggcg atgagccaga ttgcggtatt
      421 gaccgtcgct aaccggccaa gcgttcgccc tgtataccgt tcacgccatc gagcgaccgc
      481 agatcgcagg gccagcctcg gcgccatctc gtgcgtgcga tagcgatgtg gaagccctgt
      541 gacggcggcc agaattaggc ctaccgctgc gagagcgacc gtgacgacgg tgagggagtc
      601 tgagtagggc caaggcggga ctgcttccga ccatgccgcc gctcggtttg cgacgagcac
      661 aagtccaaac gatggcagaa gggacaccag gaccagtgcg gtcgagggca gtacacggtg
      721 ccgtcgcttt cgggttagca gtgcgattgc tgctgcgcac ccgaggaaaa gtagtgctgc
      781 ataaagagtg tgacgtatcc aacgaacgtt ggccggttcc gaatacagat ctgacgtgag
      841 ccgcaccttg acgattcggc ccgtgacgat cggccgcgcg gtcgactgcc ccgttccggc
      901 cgtggcgggc ataccgctga gtgccggggc gcccgtgaca accgctgtcg cggatctgcc
      961 gagcgtgtcc gttgcacgcc agcccgtgtc ggccagtgag gttgcgccga ggctcgacac
     1021 gatgtcgccg agcaaggaca ccccgccgag cgagccggtg acatggattc tgactgagtt
     1081 ggcaccctta tgaagccaag gcccgatgtt gagcaaatag gaggtctcgc cgtgcggagc
     1141 agtctggaag agaggaatcg tgttcgatcg gctggcgctg gtcgagtcga ctgcgcctga
     1201 caactgatcg atattgccga tgccgccggc gttgatgtcg gtgccattga cctggatcca
     1261 cgatattccg ttcgtccaga gtcgcagcca aacccagccg gggagagaac cgagggttag
     1321 cgtccgactc acaacggcgt cgctgatgct cgcttggaca ccgacgtcag gagcagggcg
     1381 gtcgacaaca ctctcgggcg ccatcgcacg gttggccgca cgccccgtcg ctgcggcgac
     1441 cgctgaccaa cccgcggttg ggtagctcga cgcactgaag gccggctgcc cacctagccc
     1501 attgatgtgc agtagcgcgg cgttggaggt cgcaagccag gtcgaggaag gtcctgagct
     1561 cgtgtactcc gtcgcgctct ggttgtggcc ggtcacgacg actttcgcgc ggatccttgc
     1621 tgctgaacgg tcttgcgaag tgacgtcgag gacaatcgtc gtcagaccct gcaccgcgaa
     1681 cgcgctgagg tcgacggcgt cgaccaatgg cctggcgttt gggacgtcag gctcagcgtt
     1741 gagatctccg tgatagctga gaggtaccga gatcccgttg atgtcgatcg cgacgacgtc
     1801 gggtgtgtcg taccacaatg tcatcgcggt ggctgaactc ggcacaatcc actggtcacg
     1861 aaaccatgcc cgaggcgcgt ggtcgggtgc ttggatccat gacgcatctt gaagagatac
     1921 ggggtacgac gcgtttcctg cgttgaagcc cacgcgcacc gcaaggcccg taataaccca
     1981 gcagcacatg gcgatggcac ccatcacgcc agcggtcctg atcagctgac gactcatcga
     2041 gccaactcgt agagacctgt cgcataggcc gcggcctggg tatttgcgtg gtcgggatcg
     2101 agagcgagcg ctcgctcgat gtcgccccgc gccgccagaa gatgtcggga gtcctcgtca
     2161 ataaaggcaa ggtaggtcca tgcggacgat ctcatgtcac cgctgcgtgc agtggccgct
     2221 accgtgagaa gagaggcgcg cgcatccacg tcatctcgga gtcggtatcg aatgcggccg
     2281 agggtgtacc tgaccaggtc gtcattttgt agcgcagccg gtagtccgag gagcggtgtc
     2341 ggatcggtcc tcgccactgc ctggccgact gacgccagta cgaggtcttc cgaaatcacg
     2401 acgctggtcg gatccaatgt ataagccgtc tcgagggccg ctagttgttg caccgggtac
     2461 ccggcgtccg cgtatgtctg tgccgcccag agttcgccgc cggcagatga cggaccgttc
     2521 cccgcgaatt caatctgtgc ctcggtatta acccaggagt tcgatgacgc gagtcctggc
     2581 tgcgcgacga gcgcacgttg gaagtttctt gacgcaggcg cgtacttgcc tgccgtggca
     2641 gcctgcgagc cttggtactg ctcccagcgt gcgagcagcg gcatggcaaa gaggctcagg
     2701 gccgcgacta tgacgacaag gctcatgatg aatgtacgcg agttctttga gccgggcgcg
     2761 gtcgagcgat caaatgtgcg caccgacctt gatgtcgtca gcacgacaga catgccagcc
     2821 agcgcaaata tcggaagccc tgcgcccacc gcattcagca gcagcgacca ttgcggtgtc
     2881 atcgagattc cgaacacact taccaatggc gtaccaccga cgttgtacgg gagttcgttc
     2941 gcgaactgga tgtaccttgc ctggtcgtac agaaggcttt gaatcgttgc gtacgcgacg
     3001 tatcgctcgt agacgatgac gacgaccggc aggctcgcga tacaccacgc gaccgtgcgc
     3061 gtctgcgtcg ccgcgaaccg ccccgcgcgt gtgtccttga ggggggacaa agacgagaag
     3121 cgacacaggc agcacatcgc gacgagcaac cacgcgactg atccccacgt catgccccac
     3181 ccggaccagc cggcgccgac ggcgaaccgg aggctcgccg ggccgatatc gacgaacgta
     3241 tgtctgtcga accacggcaa ctcgagtgcg acgaccgtgc cgcctatgcc ggcgaggaac
     3301 gcgaatagct ggagtcgctc cttccgtgac gcctttggta cggaatgctg tcgctgctgc
     3361 ggcacatgag catgagcgat cgtcggatcc agggccaacg acatggtcat tgcacctttg
     3421 agaccttgac caagtcgcca tccttgaggt gctgcgcgtt ggtcatgacg actaatgctc
     3481 cggccggcag tccgcttgcg agggcggccg tgctgagatc gtgcccagcc acaacgaccg
     3541 gctgctcgtg aacgcggctg tcggcgtcga cggtgaacac gaaagcagac ccgtctgggt
     3601 cgtgtacggc gatcaggggt agggccgtcg tggccgccac ttgcttgctg aaggttgcgt
     3661 acacctgcat gccgacgcgt agcagtggac tgacggtatt caggacaatc gtgaccggca
     3721 cattgaggcc ttggttgctg ctgccagggc taatcgcctg tacggacgca gtcgcggtta
     3781 ccgacggtag ctgtgggaac gtcagtgcca cgccctcacc gacgctgatc gtgccgacct
     3841 gcgtggctgg cactgcgatt gtggctcgca actccgacaa cgagatgatc tctgcgatgg
     3901 gtcggccgga caggatgacc gagccgacgt tgccgaacag gttcgagatg actccactcg
     3961 tcggcgccac gattgtcgat gaatattgca acgctgcggc gagatgctgc tcttcgacct
     4021 cgagagtcgc ttgaagaaca gccttttgga ccgccgttgc cgccacggat acggcgacct
     4081 tgtcgttgtt caccacagcc tggagctggt ctacatagtc ctgaatcgca gtggggtcga
     4141 tagttgcgat cgggtcgccg gcgcgaacct gggtgccgac gacgactgac actgacttca
     4201 cgatgatggg gtcgctcact cccggaaagc tcaccgggac ggtggcgtcc ttgatcgcac
     4261 caagtgctcc ggtcccgttg gcagtgacct ggacgaatcc tggagcagcc tttgtagcca
     4321 ccacgactgt cggcgacgac ttgaggcgca acgccacggc gatcgtcgtg agggagccga
     4381 cgacgataac gagaacgagc gccaaccctt gccagcgcct gcgcggatga ggcgcagttg
     4441 ggcgcggctc ggggcctggt agggaggtct cctcgcgcac gagcagctgg tcggcggagg
     4501 gcctggtcga ttcagatagc gtcattttct caccacgtga aagtcgtagc cgggttccat
     4561 gaaggcgtac accagtgcgg cggtctcgag cagcgcaaag gcaggtaaga gcaggagttg
     4621 ccctacgtag agacacaatc tgcgccaacc ccagcgcacc cgatgatgct gaaggttcag
     4681 ctggagtccg acgcagtagt gggtggcgaa gatggcgaac gatgcgaccc cggcgtagaa
     4741 cagcgctgtt ggtggccgcg cgtcggtcca gagctgaaac agaacgccga agatcgacaa
     4801 cggcatggcc gcccacgtgg cgaccaacag tgcgagccag aaccgatgtt gcgccgcgac
     4861 gggcgcgtgc aacacgaccc gcacgagccc gaggaaccag cgccggcgct gcttgacgaa
     4921 gtctcccgcc gactgggtgg actgttcgac aacgaagccg tcgatccagc gagtgcgcac
     4981 gcctctgtcc atctcgagaa gtgcccacca agcatcctcg gtgatggaac cttgcggccc
     5041 caggtcaaac ccgacggccc cctcgacatc gttgcgagcg agaataaatg aaccatggag
     5101 accgaagacg gcccgaccga tccgctgctg caggtagtac cggcctaggt cctcgccagt
     5161 gcgcaaggtg tcggcaaggg tcaacaacgg atgccgcctg aagtcgcggt ggtaaagaat
     5221 cgcaccctgg ccgaccctca actgacctgt gcgttcctcc tccacgatcg cttcagcgat
     5281 tcccatgatc actgaagtcg taatgtgcga ctcttcatct aggtggaaga tccaggcgtc
     5341 ggacggaatg gcggatgccg tcagcgcgta atggagagct cgagccttgt atcgtgcccc
     5401 gccgggagtc tggtattcag tcggcgtaac cagctgtacc accgatggat cgatgtcatc
     5461 gactatgcgg tctgttgcga gctcgatcac gtactcgaag accggcagcg cactcatctc
     5521 cgcttgaaca gaccggatcg accatcggag cgcgtcgagg ttctgcccgc gcgtcacaat
     5581 ccgaaagctc accagccggc cgttcatctc atgcgcgagc gtcgagaccg gggtgtgagg
     5641 ttgctggcga ggccaaagca gcccgatcaa accgatcgct gcggagacga actgaatagc
     5701 ccaaaatacg ctcaacagat ccagcgtgac ttgcgacgtt gtccccgatc cgtgcccttg
     5761 aatgatcggt cgcgcagcgg ccagcacgaa gaacagggct cccacccagc acaccaccgc
     5821 gaaacggaat gctcgagatc caggtcgcat cgtgcgaggt tcgagctcgg taccgtccga
     5881 ggcgatcagc cgtgggtagg tttggctgtg ccggccaccc gacttgctta accccgcagc
     5941 gcgggcacct atccggaact cgacaggcct gagcagctct tcgtcaaccg tcacgcgcac
     6001 cccc
//
LOCUS       DASWVF010000422         5023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163889, whole genome shotgun sequence.
ACCESSION   DASWVF010000422 DASWVF010000000
VERSION     DASWVF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163889"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VGD55_07620"
     CDS             complement(<1..413)
                     /locus_tag="VGD55_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0870252.1"
                     /translation="MKDASTTVEAGGITVTISGTDLVMAARNATSALVDAGVPKSLMA
                     KDPGLWGPDAAELAATRLGWLDLPNSSRQYIPELRDLHARLATDGIDHVVLAGMGGSS
                     LAPEVIAATAGVRLTVLDTTDAGQVAAAIADRLDRT"
     gene            complement(419..1564)
                     /gene="tal"
                     /locus_tag="VGD55_07625"
     CDS             complement(419..1564)
                     /gene="tal"
                     /locus_tag="VGD55_07625"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY0870253.1"
                     /translation="MASDASSPDPLAQLSAAGVAVWLDDLSRERLSSGNLAALIRDRH
                     VVGITTNPTIFQKAISGADAYATQLHDLATRGVDVGDALRLITTSDVRSGCDVLRPAY
                     DASDTVDGRVSIEVDPRISGDAAKTIAEARSLWWMVDRPNLFIKIPATLESLPAISDA
                     LAEGISVNVTLIFSLERYGLVIDAFFDGLERARAAGIDLAAIGSVASFFVSRVDTEID
                     KRLDKIGTDEAKALRGKAAVANARLAYELYEENFAGPRWASLAASGAKVQRPLWASTG
                     VKDPAYDDTMYVVDLVADHTVNTMPEPTLQAVHDHGVVSGDSIRGHYAGAREVLDQLA
                     AVGIDYDDVVLTLEREGVEKFEASWVDLIAAVNKELEQKELEQKAGS"
     gene            complement(1619..3754)
                     /gene="tkt"
                     /locus_tag="VGD55_07630"
     CDS             complement(1619..3754)
                     /gene="tkt"
                     /locus_tag="VGD55_07630"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0870254.1"
                     /translation="MSNPQASTYPETLVWSDVDAKAVDVIRALAMDAVEAAGSGHPGT
                     AMSLAPAAYLLFQRLLRHDPTEPGWVGRDRFVLSCGHSSLTLYIQLYLSGYGLTLDDI
                     AHLRQWGSLTPGHPEHGHTLGVETTTGPLGQGVGNAVGMAMAARRERGLFDPDTAPGE
                     SPFDHHIYCFASDGDLEEGVSGEASSIAGTQRLGNLTLLWDDNHISIEDDTNIAFTED
                     TVARYAAYGWHVQRVNWLDDNGSYHENVAALYDAFLAAHKVTDRPSFIALRTIIGWPA
                     PNKQNTGKAHGSALGAAEVAATKTVMGMDPDVKFDVPAAVLDHARDVIARGQALRRQW
                     QTGYDTWAADNPDAVALLDRMSRRRLPDGWESALPTFEPSDKGIATRAASGKVLTAIA
                     PVLPELWGGSADLAESNNTTPDAQPSFLPEDRQTKAFPGGPYGRVLHFGIREHAMGSI
                     LNGIALHGGTRVYGGTFLTFSDYMRGAVRLAAIMKLPVTYVWTHDSIGLGEDGPTHQP
                     IEQVAALRAIPGLDVVRPADANETAVAWRTILENADRPAGLILSRQNLPIVDRTVYAS
                     AEGVANGAYILADAGSGKPEAIIIATGSEVPIALAAREMLEGQGMGTRVVSMPCQEWF
                     LEQEPAYQQLVLPPDVLVRVSIEAAVTMGWRDFVGDHGEMVGINHYGASASGAVLFEQ
                     FGFTPDRLVAAVHSSLSKVTGIKGSTTGT"
     gene            3901..4833
                     /locus_tag="VGD55_07635"
     CDS             3901..4833
                     /locus_tag="VGD55_07635"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0870255.1"
                     /translation="MTLTVPGVKIAGTPPPAPRSVRQLVGAYVALTKPRIIELLLVTT
                     VPAMILARRGLPSLWLIVATLIGGSLAAGSANAINCYVDRDIDKVMRRTSRRPLARAL
                     VQPAEALRFGIVLGVAATLMLGFTVNWPSALLADAAILFYVFVYTLGLKRRTPSNIVI
                     GGAAGCFPVLIGWSAVTGGVGWPAVVLFGVIFAWTPPHFWALAMKFRDDYACAGVPML
                     PVVADATTVARKIVTYSYVMVAVSFVLVPVGHAGLPYLGVAAVLGGWFIVEAHRLSAR
                     AKAGEDPRPMRLFHMSITYLTLLFAAVALGQFVR"
     gene            complement(4843..>5023)
                     /locus_tag="VGD55_07640"
     CDS             complement(4843..>5023)
                     /locus_tag="VGD55_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870256.1"
                     /translation="LWGVDDVASAMARAVEHGAVEQGPATDVGDGIVTGSVRTPGGVI
                     LGFIYNPHFHAGAPT"
BASE COUNT          775 a         1621 c         1695 g          932 t
ORIGIN      
        1 gtgcggtcga ggcggtcagc gatcgctgcg gcgacctgcc cggcgtcggt ggtgtcaaga
       61 acagtgagcc ggacgccggc ggtggccgcg atcacttcgg gcgccaacga cgacccaccc
      121 atgccggcaa ggaccacgtg gtcgatgccg tcggttgcca accgcgcatg caggtcgcgc
      181 agttctggga tgtattgccg tgagctgttc ggcaggtcga gccagccgag cctggtcgct
      241 gctagctcgg cggcgtcagg accccacagg ccgggatcct tggccatcag gctctttgga
      301 acgccggcgt cgacaagtgc ggaggtcgcg ttgcgcgcgg ccatgacgag atcggtgccg
      361 ctgatcgtca ccgtgatgcc gccggcctcg actgttgttg atgcgtcctt catgccggtc
      421 agcttcccgc cttctgttcg agttccttct gttcgagttc tttgttgaca gcagcgatga
      481 gatcgaccca cgacgcctcg aacttctcta cgccttcgcg ctcgagcgtc aagacgacgt
      541 cgtcgtaatc gatgcctact gcggcgagct ggtcgagcac ctcccgcgcc ccggcgtagt
      601 gcccgcggat cgagtcgcca gaaaccacgc catgatcatg cacagcttgc agagttggct
      661 cgggcatggt gttcacggtg tggtcggcga cgagatcgac cacgtacatc gtgtcgtcgt
      721 aggcagggtc cttcacgccg gttgacgccc acagcggtcg ctgtaccttc gcgccggacg
      781 ccgcaagcga tgcccagcgc ggccctgcga agttctcctc gtagagctca tacgcgagcc
      841 tggcgttggc taccgcggcc ttgccgcgca gtgccttggc ctcatcggta ccgatcttgt
      901 cgagacgctt gtcgatctcg gtgtcgaccc gcgacacgaa gaacgacgcg acggagccga
      961 tcgcggcaag gtcgatcccg gccgcgcgag ctcgttcgag cccgtcgaag aacgcatcga
     1021 tgaccaagcc gtagcgctcg agagagaaga tcagcgtgac gttgacgctg atgccttctg
     1081 ccaacgcgtc gctgatggcc ggcaggcttt cgagggttgc cgggatcttg atgaataggt
     1141 ttggtcgatc gaccatccac cacagcgatc gggcttccgc gatcgtcttc gcggcgtcac
     1201 ctgagatccg cgggtcgacc tcgatcgaca cccgcccgtc aaccgtgtcg ctcgcgtcat
     1261 acgcggggcg cagcacgtcg caacccgagc gaacgtcgga ggtcgtgatg agacgcaacg
     1321 cgtcgccgac gtcgactccg cgggtggcga ggtcatgcag ttgcgtggca taggcgtcag
     1381 caccgctgat cgccttctga aagatcgtcg ggttggtcgt gatgccgacc acgtggcgat
     1441 cgcggatcaa ggctgcaagg tttcctgagc tcagtcgttc acggctgagg tcgtcgagcc
     1501 agacggcgac gccggctgcg ctgagctggg cgagcggatc agggctggat gcgtcggatg
     1561 ccatgggatg ctccctggtc tgggtgtctg cgcgggttct gcgtggtggg tttgtgtgtc
     1621 aggtgccggt ggtgcttcct ttgatgcccg tgacctttga caggctcgag tgcacggccg
     1681 cgacgagcct gtcgggggtg aacccgaact gctcgaagag gaccgcgcca gacgcgctcg
     1741 cgccgtagtg gttgatgccg accatctcgc cgtggtcgcc gacgaagtcg cgccagccca
     1801 tggtgactgc ggcctcgatg gagacgcgca ccaacacgtc gggcgggagc acgagttgct
     1861 ggtaagcggg ctcctgctcc aagaaccact cctggcacgg catcgaaacg acgcgggtgc
     1921 ccatgccctg accttcgagc atctcgcggg ccgcgagggc gatcgggacc tcgctgcccg
     1981 tggcgatgat gatcgcctcg ggcttgccag aacctgcgtc ggcaaggatg taggccccgt
     2041 tcgccacgcc ttcggctgag gcgtagaccg tacggtcgac gatgggcagg ttctgccgac
     2101 tcaaaatcag gccagccggg cggtcggcgt tctcgaggat cgtccgccag gcgacagcgg
     2161 tctcgttcgc gtcagcaggc cggacgacgt cgaggccggg gatcgcccgc aatgcggcca
     2221 cctgctcgat cggctggtgc gtcgggccgt cctcgccgag gccgatcgag tcgtgcgtcc
     2281 agacgtaggt aacggggagc ttcatgatgg cggccaatcg cactgcgcca cgcatgtagt
     2341 cgctgaaggt caggaacgta ccgccgtaca cgcgggtgcc accgtgcaac gcgatgccgt
     2401 tgaggatcga acccatcgcg tgctcgcgga tgccgaagtg cagaacccga ccgtaggggc
     2461 ccccagggaa ggccttcgtt tgcctgtctt caggcaggaa cgagggctgc gcgtctggcg
     2521 tcgtgttgtt gctctcggcg aggtcagctg agccgcccca cagttcggga agaacagggg
     2581 cgatggcggt gagcaccttg cccgacgcgg cgcgggtggc aatccccttg tcactgggct
     2641 cgaacgtagg cagcgccgac tcccatccgt cgggcagccg acggcgcgac atccggtcga
     2701 gcagggcgac cgcgtcgggg ttgtctgcag cccaggtgtc gtagccggtc tgccactgcc
     2761 ggcgcaatgc ctggccgcgc gcgatgacgt cacgggcgtg gtccaaaacc gcagccggta
     2821 cgtcgaactt cacgtccgga tccatgccca tgactgtctt cgtggcggcc acctcggcgg
     2881 cgcccagcgc cgagccgtgg gccttgccag tgttctgctt gttcggagcc ggccacccga
     2941 tgatggtgcg aagtgcaatg aacgatggcc ggtcggttac cttgtgcgcc gcgagaaacg
     3001 cgtcgtaaag cgcggcgacg ttctcgtgat aggagccgtt gtcgtcgagc caattgaccc
     3061 gctgaacgtg ccagccgtac gcggcgtacc gggcgacagt gtcttcggtg aacgcgatgt
     3121 tggtgtcgtc ttcgatcgag atgtggttgt cgtcccacaa cagagtcagg ttgccgaggc
     3181 gttgcgtccc ggcaatgctc gacgcttccc cgctgacgcc ctcttcgagg tcgccgtcgg
     3241 acgcgaagca gtagatgtgg tgatcgaacg ggctttcacc aggagccgtg tcagggtcga
     3301 acaggccacg ctcgcggcga gccgccatcg ccatgccgac ggcgttgccg acaccctgac
     3361 ccagcggtcc ggtcgtggtc tcgacgccga gcgtgtgccc gtgttcgggg tgtcccggcg
     3421 tcaggctgcc ccactggcga aggtgggcga tgtcgtcgag cgtcagcccg tagcccgaca
     3481 gataaagctg gatgtacagc gtcaggctgg agtgcccgca cgagagcacg aaccgatcgc
     3541 ggccgaccca cccgggctcc gtcgggtcgt ggcgcagtaa tcgctggaac agcagatagg
     3601 ccgccggggc caggctcatc gcggttcccg ggtggccaga accggccgcc tcaaccgcgt
     3661 ccatcgccag cgccctgatg acatcgacgg ctttcgcgtc aacgtcggac cacacaagag
     3721 tctcgggata agtgctcgcc tgcggattcg acatgggctc ctcttgtcct gtcccgaccc
     3781 tgtccgtgca acgtgatttc ccgactgtca ccctacccat gcgcgcgcgc aagccccagg
     3841 gacagctggt ccaaatgacc gtcggtagga tgcccgagtg ccgatgctcg cggaatccgt
     3901 atgactctca cggttcccgg cgtcaaaatc gcaggcacgc ccccacctgc gccccggtcg
     3961 gtccggcagc tcgtcggcgc atatgtcgcg ttgacgaagc cgcgcatcat cgagttgctc
     4021 ctggtcacca cggtgccggc gatgatcctg gcccggcgcg gcctgccgtc gctgtggttg
     4081 atcgtcgcca ccctgatcgg cgggagcttg gccgccggta gcgcgaacgc catcaactgt
     4141 tacgtcgatc gcgacatcga caaggtcatg cggcgcacct cgcggcgtcc cctcgctcgg
     4201 gcccttgttc agccggccga ggcgttgcgg tttggcatcg tgctgggtgt cgcggcaacc
     4261 ttgatgctcg ggttcaccgt caactggccg tcagccctgc tcgccgacgc ggcgatcctg
     4321 ttctacgtct tcgtgtacac gcttggtctg aagcggcgga cgccgtcgaa catcgtcatc
     4381 ggtggggcgg cgggctgctt ccccgtgctg atcggctggt cggcggtcac tgggggagtc
     4441 ggctggcccg ccgtggtgct gttcggggtg atcttcgcct ggacgccacc tcatttctgg
     4501 gccctggcga tgaagttccg cgacgattac gcctgcgccg gagtgccgat gctgccggta
     4561 gttgccgacg ccaccacggt ggcacgcaag atcgtcacct actcctacgt gatggttgcc
     4621 gtctcattcg tcttggtgcc ggtcgggcac gcgggcttgc cgtacctcgg tgtcgcggcg
     4681 gtgctgggcg ggtggttcat cgtcgaagca catcggctct cggcacgcgc gaaggccgga
     4741 gaggacccca ggccgatgcg gctgttccac atgtcgatca cttacttgac gttgttgttc
     4801 gccgccgtcg ctctcgggca gtttgtaagg taggccagtt cgtcaggtag gcgcgcccgc
     4861 atgaaagtgc gggttgtaga tgaagccgag gatcacgcca cccggggtgc gcacgctgcc
     4921 ggtcacgatc ccgtcaccga catcggtggc tggcccttgc tcgactgcac cgtgttcgac
     4981 cgcccgcgcc atcgccgagg cgacgtcgtc gacaccccac aac
//
LOCUS       DASWVF010000423         4457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_316694, whole genome shotgun sequence.
ACCESSION   DASWVF010000423 DASWVF010000000
VERSION     DASWVF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316694"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VGD55_07645"
     CDS             <1..317
                     /locus_tag="VGD55_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870257.1"
                     /translation="GELLRDHLGPGVELSVAGADLISSHTDYLAGHLAPLLLPPDVLV
                     VATSGVVDLATSIIYTATGDGGTALGIGATDLLSLSHAIVLPASNMSHPNAIEAVAAS
                     SS"
     gene            353..826
                     /locus_tag="VGD55_07650"
     CDS             353..826
                     /locus_tag="VGD55_07650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0870258.1"
                     /translation="MPAGRRPVPRLRRVEETSAGGLVVDAGIPGIRGRRAALIARHDR
                     RGRLVWSLPKGHVEEGETPEVAAVREIAEETGIAGRVVAPLGTIDFWFVAEGRRIHKT
                     VHHYLLLAEGGELSDEDIEVVEVAWVPLEELAQRLAYDDERRLVDQVPGLLADIA"
     gene            871..3195
                     /locus_tag="VGD55_07655"
     CDS             871..3195
                     /locus_tag="VGD55_07655"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEY0870259.1"
                     /translation="MLAISALCGTGIAAEATATARAAHAAVSAAPSPTPTSSNAPNRV
                     SITLETLEPKAVQPHDTVVLDGTVTNISGGPLTGVSIALRASTHRIDTRYDLAREADP
                     GNVLGSTLSNTRQTLGSLAVDQTISWHFSLQVDRLDLPSSAADFGAYPFAVDVRSTAD
                     RTTLTTRLPTTLMWLPSGSQFVQTQISWLVPLVDGVHRGEGDTFLDDELATDLAPTGR
                     LGRLLALAATAGVPMTYLVDPALVDDATAMAGTSGQPGGEPSAGVTGVTGIASSSPKP
                     TEKASAAPKAGTKSAPGSPSASPATGYQVAGQAGAQATADGIGEAAAASWLASLRTDV
                     AAPGAALVGLPYGDTDLVAVERAGLTREIAIARSTGQSTLTADLAPPTLPNVVWPIDG
                     TLDEATLDDLAGDLVDTVVLSDTTLPPRDPNAVTGARTNLQTESGTVRAVLTDSAISS
                     MLTSPAASDGVRAAEQRFLAQTMLVTEQRPGAGSSIVVAPPRNWNPAGDFASTMLSDS
                     ATMPWLQGANLGQVAAQPADGVQRKSLVYPATAQTAELPSGELLPIIGLRGTLAACSA
                     ILGSSTAETFINTASIAILRAESSGLRGQPARSAAIRSAVQAELDAQTAKVYIVKPGL
                     ITLTSRKQKIPITVVNNLPDPVTVQIRLTAVNAARLVASPAQPFTVPGNQSRHEVLIE
                     VEATTGGRFQVSAQLWTPEANPRPFGAAVPFILNSTAYGAVALVIAATAAGLVFLLSA
                     IRIVRRIIRARRAHKQAASAPHAESEPADPSPVP"
     gene            3192..>4457
                     /gene="murJ"
                     /locus_tag="VGD55_07660"
     CDS             3192..>4457
                     /gene="murJ"
                     /locus_tag="VGD55_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0870260.1"
                     /translation="MTTAEPQESSLARSSTIMAIGTIASRGTGFIRTAVLAAALGSGA
                     LAETYNIPNTVPNAIYDLLLGGILTSVVVPLLVKAVKDDAAAGEIYAQRLLTVVAVVL
                     GICTVAGVLAAPLIIDVYARGLTGEQRSLSVTFARYFLPQVFFYGLSATMGAILNTRG
                     RFAAPMWAPVLNNIVLIGTGLLFIGLTSGQPVAKLTNLDVRLTTGEIRLVGIGTTAGV
                     VLQTLALWPSLYASGFRWRPRFDWRGNGLGEAVSLGGWVLAYVGVNQVGFAVITNLGK
                     AAGRAAANHHVAHTDTFSAYMYAYQLFQLPYAIVAVSVITALLPRMSRHAAEGRFDDV
                     RADLSTGLRTSGVAVVPAAIMLIALAPQISTVVFRGLPHTRVSPADARYIGWVLVAFG
                     AGLVPFAIFQLLLRAFYSLRDTRTPALINV"
BASE COUNT          726 a         1575 c         1400 g          756 t
ORIGIN      
        1 tcggcgagtt attgcgtgac cacctcgggc caggggtcga actcagcgtc gcgggcgctg
       61 atctcatcag cagtcacacc gactacctcg cgggacactt ggcccccttg ctgctcccgc
      121 ccgacgttct cgtggtcgca acatcagggg tcgtcgacct cgcgacctcg atcatctaca
      181 ccgcgaccgg cgacggtggt accgcgctcg ggatcggggc gaccgatcta ctgagcctca
      241 gtcacgccat cgtgttgcct gcatcgaata tgtcccaccc gaacgcgatc gaggccgtgg
      301 ccgccagctc aagctgaccg ggccgggccc ggcgggccat tacgctcgag gcgtgcctgc
      361 cggacgtcgc cccgtacctc gacttcgccg agttgaagag acctctgctg gcggattggt
      421 cgtcgacgcc ggcatcccag gcattcgtgg ccgccgcgca gcactgatcg caaggcacga
      481 ccggcgcggt cgcctcgtgt ggtcgctgcc gaagggccac gtcgaggaag gcgagacccc
      541 cgaggtggca gcggttcgcg agatcgcaga ggagaccggc atcgcgggtc gggtggttgc
      601 cccgctcgga acgatcgact tctggttcgt cgccgagggc cgccggatcc ataagacggt
      661 ccaccactac ttgttgctcg ccgaaggcgg cgagctgtcc gacgaagaca tcgaggttgt
      721 cgaggtggcc tgggtgcccc ttgaagagct cgcccagcgg ttggcgtacg acgacgagcg
      781 ccgcctcgtc gaccaggtgc cggggctgct cgcggacatc gcgtgactca cgccggccga
      841 cgggcccggt tgccagtcga gctggcagtc gtgctggcga tcagtgcgct ttgtggcacc
      901 gggatcgctg cggaggcaac ggcaaccgcc cgagccgctc acgctgcggt gagcgcggca
      961 ccgagcccga ccccgacatc gtcgaatgcc ccaaaccggg tctcgatcac gctcgagacc
     1021 ctcgagccga aggcagtgca gccgcacgac accgtggtgc tcgacggcac ggtcacgaac
     1081 atctccggcg gccccctgac cggcgtgagc atcgcgttgc gcgcttcgac ccaccgcatc
     1141 gacacccggt acgacttggc ccgtgaggcc gaccccggca acgtgctcgg ttccaccctc
     1201 tccaacaccc ggcagaccct cggctcgctc gccgtcgacc agaccatcag ctggcacttt
     1261 tctttgcagg tcgaccggct agacctaccg agttccgctg ccgatttcgg tgcctacccc
     1321 ttcgccgtcg acgtgaggtc aaccgctgac cggaccacac tgacgacccg gctgccgacc
     1381 acgttgatgt ggttgcccag cggctcgcag ttcgtgcaga cccagatcag ttggctagtg
     1441 ccactggtcg acggcgttca ccggggcgag ggcgacacct tcctcgacga cgaactcgcc
     1501 accgatctcg ctccgacggg tcggctgggt cgccttctcg cgctggccgc caccgccggc
     1561 gtccccatga catacctcgt cgaccccgcg ctggtcgacg acgcgactgc gatggcgggc
     1621 accagcggcc aaccgggcgg cgagccatca gccggcgtga cgggcgtcac cgggatagcc
     1681 agttccagcc cgaagccgac cgaaaaggca tcggccgcac ccaaggctgg cactaagtcg
     1741 gccccgggat cgccatcagc atcgccggcg acgggttatc aagtcgccgg ccaggctggc
     1801 gcgcaggcca ctgccgacgg catcggcgaa gcagcagcgg cgagctggct cgcgagtctt
     1861 cgtactgatg tcgccgcgcc tggagcggca cttgtcggcc tcccgtacgg cgacaccgac
     1921 cttgtcgcgg tcgagcgagc aggcctgacc agagaaattg cgatcgcccg atcgaccggc
     1981 cagtcgaccc tcaccgccga cctcgcaccg cctaccttgc ctaacgtggt gtggccgata
     2041 gacggcacgc tcgacgaagc gacactcgat gaccttgccg gggatctcgt cgacaccgtc
     2101 gtgctcagtg atacgacctt gccgccgcgc gaccccaacg ccgtcaccgg agctcgaaca
     2161 aatctccaga ccgaatcggg cacggtgcgc gctgtgctga ccgactcggc gatcagttcg
     2221 atgctgacga gccccgcagc aagcgacggc gttcgcgctg cggagcaacg gtttcttgcg
     2281 caaaccatgt tggtcaccga gcagcgacca ggggccggca gttccatcgt cgtcgcaccg
     2341 ccgcgcaact ggaatccggc tggcgatttc gcgtcgacaa tgctctccga cagcgccacc
     2401 atgccgtggc ttcagggcgc gaatctgggt caggtcgcag cgcagcccgc cgacggcgtc
     2461 cagcgcaagt cgctcgtgta tcccgccacc gcgcaaactg ccgagctacc gagcggcgag
     2521 cttctcccga tcattggtct gcgcggcacg ctcgccgcgt gcagcgcgat cctcggctca
     2581 agcaccgcgg agacgttcat caacaccgct agtatcgcga tccttcgggc ggagtcgagc
     2641 ggactgcgcg gccagcccgc acggtccgca gcgatcaggt cggcggtgca agccgaactc
     2701 gacgcccaga ccgcgaaggt ctacatcgtc aaacccggcc tgatcacatt gaccagccga
     2761 aaacagaaga tcccgatcac ggtcgtcaac aatctccccg accccgtcac cgtgcagatc
     2821 cgcttgaccg cggtgaatgc cgcgcgccta gtggcgtcgc ccgcgcagcc cttcaccgtg
     2881 cctggcaacc aaagcaggca cgaagtgctg atcgaagtcg aagcgaccac gggtggtcgc
     2941 ttccaggtca gtgcccagtt gtggacgccg gaagccaacc ctcgaccgtt cggcgcagcg
     3001 gttccgttca tcttgaactc caccgcctat ggggcggtcg cgctggtgat tgctgctacc
     3061 gctgccggac tggtgttcct gttgtcggcg atccggattg tgcggcgcat catccgcgcg
     3121 cggcgagcgc acaagcaggc cgcgagcgca ccgcacgcgg agtccgagcc ggctgacccg
     3181 tcgcccgtgc cgtgaccacg gccgaaccac aggagagcag cctcgccaga tcgagcacga
     3241 tcatggcgat cggaaccatc gcgtcgcgtg gcaccgggtt cataagaact gccgtcttag
     3301 cagcggcgct cggcagtggc gccctcgccg agacgtacaa catcccgaac acggttccga
     3361 acgcgatcta cgacctgctt ctcggcggga ttctcaccag cgttgtggtt ccgctgctcg
     3421 tgaaggccgt caaggacgac gcggcagccg gcgagatcta cgcgcagcga ctgctgacgg
     3481 tcgtcgctgt cgtactcggc atctgcacgg tggccggcgt gttggctgcg ccgctgatca
     3541 tcgacgtcta cgcacgcgga ctgaccgggg aacagcgctc cctgtcagtc accttcgcgc
     3601 ggtacttctt gccgcaggtc ttcttctacg ggctaagcgc gactatgggc gcaattctca
     3661 acacccgtgg gaggttcgct gcacctatgt gggcgccggt gctcaacaac atcgtgctca
     3721 tcggcactgg gttgctgttc atcggtctta cgtccggtca acccgtcgcc aagctcacca
     3781 acctcgacgt ccggctcacc acaggtgaga tccgccttgt cgggatcggc acgactgctg
     3841 gcgtcgtcct gcaaaccctt gctttgtggc cgtcacttta tgcgtcggga tttcggtggc
     3901 ggccacgctt cgattggcgc ggcaacggct tgggtgaagc ggtcagcctc ggcggctggg
     3961 tccttgccta cgtcggggtg aaccaggtcg gcttcgccgt catcacgaac cttggcaagg
     4021 cggccggccg ggcggccgcc aatcatcacg tcgcccacac cgacaccttc tcggcctaca
     4081 tgtacgcgta ccagttgttc cagctgccct acgcgatcgt cgccgtatcg gtcatcacgg
     4141 ccctgcttcc gcgcatgagc cgtcatgccg ccgaaggcag gttcgacgac gttcgcgccg
     4201 acctgtcaac gggcctgcga acgagtgggg tggccgtcgt ccctgccgcg atcatgctga
     4261 tcgccttggc gccgcagatt tcgaccgtcg tgtttcgcgg cctgccccac acccgggtga
     4321 gccccgccga tgcgagatac atcggctggg tgcttgtcgc gttcggtgct ggcctggtgc
     4381 cgttcgcgat ctttcagctc ctgctgcgcg cgttctactc gctgcgcgac acccgcactc
     4441 cggcactcat caacgtc
//
LOCUS       DASWVF010000424         5292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_215287, whole genome shotgun sequence.
ACCESSION   DASWVF010000424 DASWVF010000000
VERSION     DASWVF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215287"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGD55_07665"
     CDS             <1..541
                     /locus_tag="VGD55_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870261.1"
                     /translation="AKARTVAAEVADTEKTLRGILERFRFERVELAADAEAGLARVAV
                     TVNGVTTVENDVTGHGLNQFTYAGTWGHATSNLEAAGTNSHSSMPGDTATLRFVGTRI
                     RFYGVVAPNHGTASLAVDGATPATIDQYSPERVQGGLAWESPVLARGEHTFVLTVLGE
                     ANPASRYIWVNVDRVEIDA"
     gene            855..2150
                     /locus_tag="VGD55_07670"
     CDS             855..2150
                     /locus_tag="VGD55_07670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEY0870262.1"
                     /translation="MTDAWSFETKQIHAGQHPDEATHARALPIYQTTSYTFRDTDHAA
                     ALFGLAEFGNIYTRIMNPTQATVEERVAALEGGVAALLVASGQSAETLAILNIAGSGD
                     HIVSSPSLYGGTYNLFHYTFPKLGIEVSFVDDPDDPESWRRAVRPNTKAFFGESLANP
                     KNDFLDIETVAAIAHENGVPLIIDNTVPTPYLLRPFEWGADIVVHSATKYLGGHGTAI
                     AGVIVDGGRFDWAAHADKFPGLTTPDPSYHGVVYTEAVGNLAYIIKARVQLLRDIGPA
                     VAPFNAFLIAQGLETLSLRIERHVANAQKVAEWLEKRDEVERTIYAGLPSSPWYERGK
                     KYSPKGAGAIVSFEIKGGLEAGKRFVNALELHSHVANIGDVRSLVIHPASTTHSQLSP
                     EEQLATGVTPGLVRLAVGIEGIEDILADLDLGFRAAKEA"
     gene            2156..3346
                     /locus_tag="VGD55_07675"
     CDS             2156..3346
                     /locus_tag="VGD55_07675"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0870263.1"
                     /translation="MPSVGGLPPSGAWKEGDHPGDRRFADLGAFDLELGGHLPNVRVA
                     YETWGELNEDSSNAVLVLHALTGDSHVVGPAGPGHVTAGWWDGLIGPGAPIDSGTWFV
                     VAPNVLGGCQGTTGPSSLQSATEQSSLRDTEKQWGSAFPRITVRDQVDVEAALATHLG
                     ITKWASLVGGSMGAMRVLEWLVGYPSRTTAALVLAVGATATGDQIGTQSAQLRAIRSD
                     PNWRGGDYYDAANGDGPADGMGLARRIAHLTYRSAEELQTRFGNEPQDGEDPLFDGRF
                     AVESYLDHQATKLVGRFDAGTYVALTDAMNTYDVGRRRGGVAAALASITAPVIVAGVD
                     SDRLYPIGLQRELAALIPTTVSGLRVVHSDFGHDGFLIERDAVGALVRESLAIADRSM
                     APCR"
     gene            complement(3351..3812)
                     /locus_tag="VGD55_07680"
     CDS             complement(3351..3812)
                     /locus_tag="VGD55_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870264.1"
                     /translation="MSVKVPVKTQENSPVMADAVIAQVIQLKDPDEAISSAVWWRVAE
                     LLRTCTTATTSGPHLTTTLLEHAFSATDDVLSSLSADSDPSNAASVLDSALPLGSGYD
                     ALAATRRSCLRLARLLFSLDATTPAVLATRVNQLRRALTAWRFEALDAFSS"
     gene            complement(3923..>5292)
                     /gene="glpK"
                     /locus_tag="VGD55_07685"
     CDS             complement(3923..>5292)
                     /gene="glpK"
                     /locus_tag="VGD55_07685"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY0870265.1"
                     /translation="TRVVIETTLLSAGLHASDVAAVGITNQRETTVVWNRRTGRPYHN
                     AIVWQDTRTDAIAARLEHSGAGDVIRHKAGVPPATYFSAGKLQWILENVDGVRADAER
                     GDAVFGTIDTWLLWNLTGGTDGGLHVTDPTNASRTMLMNLETLQWDDELLAFFDVPRS
                     MLPQIVPSSGPAESVGGAVSRRDGPLGGEVRLAGVLGDQQAAMVGQACFDVGNAKNTY
                     GTGNFLLLNTGTAIARSKYGLLTTVCYRFGTEPARYALEGSIAVTGSAVQWLRDQLGI
                     ISSAAHSESLAREVEDAAGLYFVPAFSGLFAPHWRSDARGVIVGLSRFHTSAHVARAT
                     LEAICYQTREVVEAMQDDTGVAFTELRVDGGVTANELCMQLQADILGVPVSRPAVAET
                     TALGAAYAAGLSVGFWASIEQVRTHWREDRRWVPTWQEDRRAEGYRGWQKAVDRTLRW
                     VEPDD"
BASE COUNT          921 a         1684 c         1761 g          926 t
ORIGIN      
        1 tgccaaggca cgcacggtcg ccgcagaagt tgcggatacg gagaagacgc tgcgcgggat
       61 actcgagcgc tttcgcttcg agcgcgtcga gctggctgct gacgccgagg caggccttgc
      121 tcgtgttgcg gtcactgtca acggcgttac gacggtcgag aacgacgtga cgggtcatgg
      181 gctcaaccag ttcacgtacg ccggcacgtg gggtcacgcg acgtcgaacc tcgaggccgc
      241 cggcaccaac tcgcactcga gcatgcccgg tgatacggcg accctgcggt tcgtgggcac
      301 gcgcatcagg ttctacggcg tcgtggctcc gaaccacgga acggcgtcac ttgcggtgga
      361 cggcgcgacg ccggcgacca tcgatcagta ctcgcccgaa cgggtccagg gcggcctcgc
      421 ctgggagagt cctgtactag ctcgtggtga gcacacgttc gtgctaaccg tgctggggga
      481 ggccaaccca gcctcacgct acatctgggt aaatgtcgac agggtcgaga tcgacgccta
      541 gaactgacgg atcagggcct tggcgagcct gcgatcgctt gcggcacccg ccaaccgatc
      601 aaaatggtgc gtatccgcct cgacgcaccc acggaccatg tggttgcatg gcgccatgat
      661 gcgaatgcca ctgctgctcg acccgttctg ggtcggccgt cgcgcgcatc atttcgacca
      721 gcgctgttgc tgaccgcgca gcgaactcgc gcgatatcac cggcgcgagc ggagattcct
      781 gagcgttagg gcctgttgtg cccgcactca atcgcaccaa ctcacatcaa aagcgacttc
      841 ccgagaggat tccaatgact gacgcttgga gtttcgagac caaacagatc catgcgggcc
      901 agcaccccga cgaagccacg catgcgcggg cgctgccgat ctatcaaacg acgtcgtaca
      961 cgttccgaga caccgaccac gccgcggccc tcttcggtct cgcggagttc ggcaacatct
     1021 acacgcggat catgaacccg acgcaggcaa ccgtcgaaga acgcgtagcg gcgctagagg
     1081 gcggggttgc cgcgcttctc gtggcgagtg ggcagtcggc tgagacgttg gcgatcctca
     1141 acatcgctgg gtcgggcgac cacatcgtgt cgagccccag cctttacggc ggcacgtaca
     1201 acctcttcca ctacacgttc ccgaagcttg ggatcgaagt gtcgttcgtc gacgaccccg
     1261 acgaccccga gtcgtggcgg cgcgccgttc gcccgaacac gaaggcattc ttcggtgagt
     1321 cgctcgcgaa ccccaagaac gactttctcg atatcgagac agttgcggcg atcgcccacg
     1381 aaaatggcgt gccgctcatc atcgacaaca cggtgcccac gccgtacctg ctccggcctt
     1441 tcgagtgggg cgccgacatt gttgtgcact ccgcgacgaa gtacctgggt gggcacggga
     1501 cggcgatcgc cggcgtcatc gtcgacggcg gccgattcga ctgggccgcg catgccgaca
     1561 agttccccgg gctgaccacg ccagacccga gctaccacgg cgtcgtctac accgaggctg
     1621 tcggcaacct ggcctacatc atcaaggcgc gagtgcagtt gcttcgcgac atcggtccgg
     1681 cagttgcgcc cttcaacgct ttcctgatcg cgcagggtct cgagaccttg tcgctgcgca
     1741 tcgagcggca cgtcgcgaac gcgcagaagg tggccgagtg gctcgagaag cgagacgagg
     1801 tcgagcgcac catctacgcg ggtttgccgt cgtctccgtg gtacgagcgc gggaagaagt
     1861 attcaccgaa gggtgcaggc gcgattgtct cgttcgagat caagggcggt ctcgaagcgg
     1921 gcaagcgctt cgtcaacgcg cttgaactgc acagtcacgt tgccaacatc ggcgacgtga
     1981 ggtcgctcgt catccacccg gcatcgacca cgcactcgca gttgtcgccc gaggagcagt
     2041 tggcaaccgg tgtgacgccg ggtcttgtcc ggctcgcggt cggtatcgaa ggtatcgaag
     2101 acatcctcgc cgacctcgac ctcggcttcc gcgccgcgaa ggaggcctga gtccggtgcc
     2161 atcagttggt ggtctccctc catccggtgc atggaaggag ggagaccacc ctggcgacag
     2221 gaggttcgcc gatctcgggg cgttcgacct cgagctcggc gggcacctgc cgaatgtgcg
     2281 cgttgcgtac gagacctggg gcgagctgaa tgaggattcg tcgaacgcag tgctggttct
     2341 gcacgcgctg actggcgatt cgcacgtcgt cggacccgct ggcccggggc acgtcacggc
     2401 gggctggtgg gacgggctca tcggtccagg tgccccgatc gacagcggta cgtggttcgt
     2461 ggtcgcgccc aacgtccttg gtggctgcca agggacgacc gggccttcat cgctgcaaag
     2521 cgccactgaa caatcgtcgc tccgcgacac tgaaaagcag tggggctcgg cgtttccgcg
     2581 catcacggtt cgcgatcagg tcgacgtcga ggcagcgctc gcgacccatc tcggcatcac
     2641 caaatgggcg tcgctggtcg gcggatcgat gggcgcgatg cgggtgctgg agtggctcgt
     2701 cggatatccg agccggacga cggctgcgct ggttcttgcc gtgggcgcta ccgctaccgg
     2761 cgaccagatc ggcacccaaa gcgcgcaatt gcgagccatc aggagtgatc cgaattggcg
     2821 tgggggcgac tactacgacg ccgccaatgg tgacgggcca gccgatggca tgggccttgc
     2881 ccgcagaatc gcccatctca cgtaccgcag tgccgaggag ttgcaaacgc gtttcggcaa
     2941 cgagccccag gacggtgaag atccgttgtt cgacggcagg ttcgcggtgg agtcgtatct
     3001 cgaccaccag gcgacgaagc tggtcggccg gttcgatgcg ggcacgtatg tggcgttgac
     3061 cgatgcgatg aacacatacg atgttggccg gcgtcgcgga ggtgtcgccg ccgcgcttgc
     3121 ctcgatcact gcgccggtga tcgttgcggg ggtcgactcc gaccgcctgt atccgatcgg
     3181 actccagcgc gaactcgcgg cgctgatccc gacgacagtg agtggcttac gcgttgtgca
     3241 ttcagacttt ggccacgacg gttttctgat cgaacgcgac gccgtcggtg ctctggtcag
     3301 agaatcgctt gcgatcgccg atcggtcgat ggcgccctgc cgctgagtgc ctagctgctg
     3361 aacgcgtcga gagcttcaaa tcgccacgcc gtcaacgcgc gacgcagttg gttgacccga
     3421 gtcgccaaca ccgcgggcgt ggtggcgtcg agggagaaca gcaggcgagc gaggcgcagg
     3481 caactgcgac gggtcgcggc gagggcgtcg tagccggagc ccaagggcaa ggcagagtcg
     3541 agaacggacg ccgcgttgct cggatcgctg tcggcgctca gcgacgacag cacgtcgtcg
     3601 gtcgcgctga aggcatgctc gagcagcgtg gtggtcaagt gcgggccgct agtcgtggcc
     3661 gtcgtgcagg tgcgcagcag ctcagcgacc cgccaccaga ccgcactcga gatggcctcg
     3721 tcgggatcct tcaactggat cacctgggca atcacagcgt cggccatcac tggcgagttt
     3781 tcttgcgtct tcacgggcac tttcacgctc atctgaagct cctctcgcgg attcgtcctg
     3841 cgacccactc tgtttctcag aaccactgtg gtccgggacc ctgaagccct cctgcgtacc
     3901 cgctgagaat ctcctggcag aactagtcgt cgggctcgac ccatcgcagc gtgcggtcga
     3961 ccgccttctg ccagccgcgg tagccctcgg cgcgccgatc ctcttgccag gtcggaaccc
     4021 atcggcgatc ttctcgccag tgagtgcgaa cctgctcgat cgatgcccag aatccgaccg
     4081 aaagcccagc ggcgtacgcg gcgccgagcg cggttgtctc ggcgaccgca ggtcgtgaga
     4141 cagggacgcc gagaatgtcg gcctggagtt gcatgcaaag ctcgttcgcg gtgacgccgc
     4201 catcgacgcg aagctcggtg aaggcgacgc cggtgtcgtc ttgcatcgcc tcgacgacct
     4261 cacgcgtctg atagcaaatg gcctcgagcg ttgcgcgcgc gacgtgagcg ctggtgtgga
     4321 atcgcgacag cccgacgatc acgccgcgcg cgtcggagcg ccagtgcggg gcgaacaatc
     4381 cggaaaacgc gggcacgaag tacagtccgg cggcgtcctc gacttcgcgc gcgagtgact
     4441 cactgtgcgc agcgctggag atgatgccga gttggtcgcg caaccactgc acggccgagc
     4501 ccgtgaccgc gatcgaaccc tcgagtgcat agcgagccgg ttcggtgccg aagcggtaac
     4561 agaccgtggt gaggagtccg tacttcgagc gggcgatcgc ggtgccggtg ttcagaagaa
     4621 ggaaattgcc tgttccatag gtgttcttgg cgtttccgac gtcgaagcag gcttggccga
     4681 ccatggcggc ctgctgatct ccgaggacgc cggccaggcg gacctcgccg ccgagggggc
     4741 cgtcgcggcg acttaccgca ccgccgacag actcggccgg ccccgacgac ggcactatct
     4801 gcggcagcat cgacctcggc acatcgaaaa acgcgagcaa ctcgtcgtcc cattgaagtg
     4861 tttctagatt catcaacatg gtgcggctgg cgttggtcgg gtcggtcaca tgcaggccgc
     4921 cgtcggtgcc gccggtgagg ttccagagca gccaggtatc gattgtgccg aacaccgcgt
     4981 caccgcgctc ggcgtcggcg cgcacgccgt cgacgttctc gaggatccat tgcagcttgc
     5041 ccgcggagaa gtaggtcgcg ggaggtacgc ccgccttgtg gcggatcacg tcgcccgcgc
     5101 cgctgtgttc gagccgtgcc gcgatggcgt cggttctggt gtcctgccac acgatggcgt
     5161 tgtggtacgg gcggccggta cggcggttcc agaccaccgt ggtttcgcgc tggttggtga
     5221 tgccgacggc tgctacgtcg gaagcgtgca ggccggcgga aagcaacgtc gtctcgatga
     5281 cgacccgcgt gc
//
LOCUS       DASWVF010000425         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_470027, whole genome shotgun sequence.
ACCESSION   DASWVF010000425 DASWVF010000000
VERSION     DASWVF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470027"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VGD55_07690"
     CDS             complement(<1..703)
                     /locus_tag="VGD55_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870266.1"
                     /translation="MRTAAIPDAAPLPPLLPTTVGWRWPWLVGTLVLIVTAGGLLLPA
                     LEMGFQLGTSDDEQYTSWVGTPRVEAYFAVIVVSLATFGLLCFRRWNQRTRNVMFAVC
                     ALFGVACGGWGVHESGARRYIGPALAAAVKAVPHPAGATSSGPITVNSDEPAAYLDLL
                     GEPSAWRSWKLPTDSASAACAAVTQMVGGGRGWEARVDRFYCEFDRKEGRVLVRVSDN
                     QLGSTANTIYVTAYPN"
     gene            912..1562
                     /locus_tag="VGD55_07695"
     CDS             912..1562
                     /locus_tag="VGD55_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870267.1"
                     /translation="MRVNTLAATVGLAVLALCATACKSSSHAAGAAAASNPPATETQA
                     DSDNPNVSASTSASQASAAAASPTAPASASPSATASSAPAASAGGAINACSLLTGAQA
                     SALSGRAFGPGVASVLAPGQDQCEYPYNGAGVSLDVIVYEPSSGVTFAMSQSVLGGVG
                     TVMSVSGVGDKAIFAGIELDVMAGKYLFAVQGAGGLNESAGAVAIAKQIAPELASK"
     gene            complement(1609..>3678)
                     /gene="rho"
                     /locus_tag="VGD55_07700"
     CDS             complement(1609..>3678)
                     /gene="rho"
                     /locus_tag="VGD55_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0870268.1"
                     /translation="DDAETGGARTKRRAAGTGLSSMVLPELKALAATMGISGTAAMRK
                     GDLIAAIQGGQQATTNGAGKTRSAPTTRSASTEAAGSTRPARSRRPAGAASEAPASTD
                     FERNGSDHHVAERESAERSASESSRPEGGATAGAESTDGAGQQRGGQAVPSQGGYRQD
                     RPDRQRGGQNRNRDRSERDRGDRADGGQAGQSDQPQQAQGGGQNNQNSQNSQGDRPRQ
                     NQGGQDRQGGQDRQGGQDRQGGQHNQGGQNRQGGQNQGGQNQGGQNQGGQPRDDDEFD
                     SSGRRRRGRFRERNRSARGPGRERFGSEAEPVVSEDDILVPVAGILDILDNYAFVRTS
                     GYLPGANDIYVSLAQVRRYGMRKGDAVTGVVRQPKDNERREKFNAMVRLDTVNGTDPE
                     KAKARPEFGKLTPLYPQDRLRLEGDPGQLTTRVIDLVAPIGKGQRGLIVSPPKAGKTM
                     VLQAIANAITRNNPECHLIVVLVDERPEEVTDMQRSVKGEVIASTFDRPAEDHTMVAE
                     LAIERAKRLVELGHDVVVLLDSITRLGRAYNTAAPASGRILSGGVDSAALYPPKRFFG
                     AARNIENGGSLTILATALVETGSRGDEVIFEEFKGTGNMELKLDRKFADKRIFPAVDV
                     DASGTRKEELLMAPEELQIVWKLRRVLHALDSQQALELLLNKMKETKSNAEFLLQIQK
                     TTSMPID"
BASE COUNT          523 a         1247 c         1231 g          677 t
ORIGIN      
        1 cgttggggta tgccgtcacg tagatcgtgt ttgccgtgga gccgagctgg ttgtcgctga
       61 cgcggacaag gacgcggccc tccttgcggt cgaactcgca gtagaagcgg tccacgcgag
      121 cctcccagcc gcgcccgccg ccgaccatct gagtcacggc tgcgcacgct gccgatgccg
      181 aatctgtcgg caacttccac gatctccacg ccgaaggctc gccgagcaga tcgagatacg
      241 cggcgggctc atccgagttg acggtgattg gccccgacga cgtcgctcct gccgggtgcg
      301 gaaccgcctt gacggcggcg gccagcgcgg gtccgatgta gcggcgcgct cccgactcgt
      361 gcacgcccca accgccgcag gcgacgccga acagcgcgca caccgcgaac atcacgttgc
      421 gggtcctctg gttccaccgt cggaagcaga gaagtccgaa tgtggccagc gagacgacga
      481 tcaccgcgaa gtacgcctca acgcgggggg tgcccaccca gctcgtgtac tgctcgtcgt
      541 cgctagttcc gagctgaaag cccatctcaa gagccggcag cagtagccca ccggccgtca
      601 ctattaggac aagggtccct accaaccacg gccagcgcca acccacggtc gtgggtagca
      661 acggcggcag cggcgcggcg tccggtatcg cggcggttcg caccgtgaag gcatcgacgc
      721 ctcaagcggg gcccttgatc acgaccgcac cagcacggcc tgtctgatct tgaaatacgt
      781 gacacccctt cacctccagg gtggagaagt gtgacttgtt tgggcgtcgt ggcgaattac
      841 gcagctgtta caggtggcgg gttggcttcg agcatcggga cgttcgggcc cggccacagt
      901 ttggagcagc catgcgagtg aacacccttg ccgcgaccgt tggtctcgcc gtccttgccc
      961 tgtgcgcgac ggcttgcaag agcagttcgc acgcggccgg tgccgcggcg gccagcaatc
     1021 caccggcgac ggaaacccag gcggactcgg acaacccgaa cgtgtcggcg tccacctcgg
     1081 ccagccaggc ttctgcagcg gccgcgagcc cgaccgcccc ggcatccgcc tcgccctcgg
     1141 cgaccgcgtc gagcgcgccg gcagcatctg ccggcggcgc gatcaacgca tgctcactcc
     1201 tgaccggcgc tcaggcctcg gcattgtcgg ggcgcgcgtt tggccccggc gttgcgtcag
     1261 tgctggcgcc cggtcaagac cagtgcgaat acccgtacaa cggcgccggc gtgagcctgg
     1321 acgtcatcgt ctacgagccg agcagcggag tcaccttcgc catgtcgcag tcggtgctcg
     1381 gcggcgtggg cacagtgatg tcggtgagcg gcgtgggaga caaggccatc ttcgcgggca
     1441 tcgaactcga cgtgatggcg ggcaagtacc tctttgccgt ccagggcgcg ggcggcctga
     1501 acgagagcgc cggcgcagtt gcaatcgcga agcagatcgc gccagagctt gcttcgaaat
     1561 gatccggact tcggctaccg actcaggcgt cagacctcgt cgtccgtgct agtcgatcgg
     1621 catcgaggtt gtcttttgga tctgcaacag gaactcggcg ttgctcttcg tctctttcat
     1681 cttgttgagc agcaactcaa gcgcctgctg tgagtcgagc gcgtgcagca cccggcgcag
     1741 cttccagacg atctgcagtt cttccggtgc catcaggagt tcttccttac gggtaccaga
     1801 agcatcgacg tcgactgcgg ggaagatccg cttgtcggcg aacttccggt cgagcttcag
     1861 ctccatgttt ccggtgccct tgaactcctc gaagatgacc tcgtcaccac gcgagccggt
     1921 ttcgaccagc gctgtagcga ggatcgtgag cgagccaccg ttctcgatgt tgcgtgcggc
     1981 cccgaagaac cgcttcggcg ggtacagcgc tgccgagtcg acaccaccgg acaagattcg
     2041 accggacgcc ggagccgcgg tgttgtacgc gcggccaagc cgggtgatcg agtcgagcag
     2101 gaccacgacg tcgtggccga gttcgaccag tcgcttcgcc cgctcgatcg cgagctcggc
     2161 gaccatcgtg tggtcctcgg ccggtcggtc gaaggtcgag gcgatgacct cgcccttcac
     2221 cgagcgctgc atgtcggtaa cttcctctgg ccgctcgtcg acgagcacga cgatcaagtg
     2281 gcactcgggg ttgttgcgcg tgatcgcgtt cgcgatcgcc tgcaacacca tggtcttgcc
     2341 ggccttgggc ggcgacacga tcagaccgcg ctgacccttg ccgatcggcg caaccaggtc
     2401 gataacccgg gtcgtgagct ggcccggatc gccctcgagg cgaagccggt cttgggggta
     2461 gagcggcgtg agcttgccga actccgggcg tgccttggcc ttctccgggt cggtgccgtt
     2521 gaccgtgtcg aggcggacca tcgcgttgaa cttctcgcga cgctcgttgt ctttcggctg
     2581 cctgacgacg ccggtgactg cgtcaccctt tcgcatgccg taacggcgca cctgggcgag
     2641 ggacacgtag atgtcgttcg cgccgggcag atagcccgac gtgcggacga acgcgtagtt
     2701 gtcgaggatg tcgaggatcc ctgcgacggg caccaagatg tcatcctcgc tgacgacggg
     2761 ctcggcctcg ctgccgaacc gttcgcgacc cgggccccgc gcactgcggt tccgctcgcg
     2821 gaaacgcccg cggcgccggc gtccgctgct gtcgaactcg tcgtcgtcgc gcggctggcc
     2881 gccctggttc tggccgccct ggttttggcc accctggttt tggccaccct gccggttctg
     2941 gccgccctgg ttgtgctggc cgccctgccg gtcctgaccg ccctgcctgt cttgaccgcc
     3001 ctgcctgtct tgaccgcctt ggttctgacg gggccgatcg ccttgggagt tctgtgagtt
     3061 ctggttgttc tggccgccgc cctgagcctg ctggggctgg tcggactgtc cggcttgccc
     3121 gccgtcggcc cggtcacctc ggtcgcgctc actgcgatcg cgattgcgat tctggccgcc
     3181 gcgctggcga tccgggcggt cttggcggta cccaccctgg ctcgggacgg cctgcccgcc
     3241 gcgttgctga ccggcgccat cggtgctctc ggcgcccgcg gttgcgccgc cctcgggacg
     3301 tgagctctcg gacgcgctgc gctcggcgct ctcgcgctca gcgacgtggt ggtcggagcc
     3361 gttgcgttca aagtctgtcg aggctggcgc ctctgacgct gctccggccg gacgccggga
     3421 gcgggccggc cgggtcgaac cggcggcttc tgtcgatgca gatcgggtgg tcggcgcgga
     3481 ccttgtcttg cccgctccgt tggtggtggc ctgctggccg ccctgaatag cggcgatcag
     3541 gtcgcccttg cgcatcgcgg cggtgccgct gatgcccatc gtggcggcaa gcgccttgag
     3601 ttctggcagc accatcgagc tcaggccggt tccggccgct cggcgtttgg tgcgcgcgcc
     3661 acctgtttcg gcatcgtc
//
LOCUS       DASWVF010000426         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_163936, whole genome shotgun sequence.
ACCESSION   DASWVF010000426 DASWVF010000000
VERSION     DASWVF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163936"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGD55_07705"
     CDS             complement(<1..758)
                     /locus_tag="VGD55_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870269.1"
                     /translation="MSPRTARVTAWSVFVAAAALSAPGVVLDWEARDITSAPATGNRV
                     FDLVLETLSLSLLLVLLAFPIVGLVIATRRRTSPIGWLLLVIGLGWGLVEGTSGYADY
                     GLKHHPGSLPRADIAAAVGMGTWALPIGIIGTFLLLLFPTGHLPSRRWRPVAYAAAFA
                     IAVSTVVSLLQPGPMTSSGYPTASNPIGVESLASVLRPARAVVLLLALTMVASAASLV
                     VRFRRAPPMERLQIKWLAAAAGLVAAIYLGDTSVS"
     gene            complement(898..974)
                     /locus_tag="VGD55_07710"
     tRNA            complement(898..974)
                     /locus_tag="VGD55_07710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(938..940),aa:Ala,seq:cgc)
     gene            complement(1039..1908)
                     /locus_tag="VGD55_07715"
     CDS             complement(1039..1908)
                     /locus_tag="VGD55_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870270.1"
                     /translation="MSSFIVLGIVAIWILVLVPMWLNRHDSDSASRSMDSFSTAMRVL
                     SRRTAARADRRYLVMRRDDWGITVDNEPETSTGRRPLTLRLPVLRLPTRRQRYAPLSG
                     RARLLARRRRIALFFAVLTIGLAVSAAVFHTSWWFELGAGVALAAYVVHLRTDAIRAA
                     EMRRRRLVRTAREAAPRMSSRMAAGDYRYAARDLVAEAEPEVSLVGAVSASGTENGSR
                     WEPVAVPLPTYVSKPVVSRPAAAAPKADAESGTIDLTRPGVWVDSQVDVRHLLEDDDE
                     LAAIISRRRAVND"
     gene            complement(2238..>2729)
                     /locus_tag="VGD55_07720"
     CDS             complement(2238..>2729)
                     /locus_tag="VGD55_07720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0870271.1"
                     /translation="AAPVDAGRGYGDAVRALRRDARLGLCYPFAMLVDDRFAGQVTVG
                     AIARGSANSAYLGYWIAKPFAGRGIMPTALAMVVDHCFADAGLHRVEANIRPENAASR
                     RVVEKLGFRLEGTRERYIHIAGGWRDHLSYALTVEDVPGGLLPRWLAAGGAARLTGAG
                     NQG"
BASE COUNT          534 a          919 c          909 g          367 t
ORIGIN      
        1 gaaacagagg tgtcgccgag atagatcgca gcaacgagtc cggcggccgc ggccagccat
       61 ttgatttgca accgctccat cggcggcgcc cggcgaaacc ggacgacgag gctcgcggcc
      121 gacgcgacca ttgtcaacgc gagcagcaag acgaccgccc tggctggcct gagcacggaa
      181 gccaggcttt cgacgccgat cgggttcgac gcggtgggat atccactgct cgtcatcggc
      241 ccgggctgaa gaagcgacac gaccgtgctg accgcgatgg caaatgctgc cgcgtaggcc
      301 accggtcgcc accggcgact gggcaggtga ccggtcggga acagcagcaa cagaaaggtg
      361 ccaatgatcc cgatcgggag cgcccacgtc cccattccga ccgcagccgc gatgtcagct
      421 cgcggaagcg aacccgggtg atgcttgagt ccgtagtcgg catagccaga ggtgccctcg
      481 accaagcccc agccgagacc gatcacgagc agcaaccacc cgatcggact cgtgcggcgc
      541 cgcgtggcaa tcacgaggcc gacgatcgga aatgccagca acacgaggag caacgagagc
      601 gacagggtct cgagcaccag gtcgaacacc cgattgccgg tcgccggcgc actggtgatg
      661 tcgcgcgctt cccagtcgag cacgactcca ggcgccgaca gcgcggcagc ggcaacgaac
      721 acggaccaag ccgttacgcg cgcagtccgg gggctcattc ggcacctcct cgctgcaaac
      781 ctaggcgtcg ctcgtaagac gatgcgcatc ggacttgggc gacggcaaca gactggcgaa
      841 caccagttac gagaccgtta cgaccactca cggtcggaca cctgtgcggg gcgaaaatgg
      901 tggagctgag gggattcgaa cccctgaccc cctcgatgcg aacgaggtgc gctaccaggc
      961 tgcgccacag ccccgaaatg ggaatggcca ggctaccacc cgggtttggc gcgccctttc
     1021 agtcgttggc ggcccgactt agtcgttgac agcccggcga cggctgatga tcgcggcaag
     1081 ttcgtcatcg tcctcaagca aatgccgcac gtcgacctgg ctgtcgaccc acacgcctgg
     1141 tcgggtgagg tcgatcgtgc ccgactcggc gtcagctttc ggtgccgccg ccgcaggccg
     1201 tgaaacgacc ggcttcgaga catacgtcgg cagcgggacg gcgacgggtt cccaccgcga
     1261 tccgttctcg gtaccggatg cgctcacagc accaacgagc gaaacctcag gctcggcctc
     1321 ggcgaccaga tcgcgcgcgg cgtagcggta gtcaccggcg gccatccgcg acgacatgcg
     1381 gggagcggcc tcccgcgccg tccggaccaa tcgacggcga cgcatctcgg cggcccggat
     1441 cgcgtcggtg cgcaggtgca ccacgtacgc agcgagagcg acgcccgcgc caagctcgaa
     1501 ccaccacgag gtatggaaaa ccgcggccga gacggcgagc ccgatcgtga gcaccgcgaa
     1561 gaagagcgcg atccggcgcc ggcgggcgag caaccgggct cgaccgctta acggcgcgta
     1621 gcgctgacgg cgggtgggca ggcggagcac tggcaaacgg agcgtcagcg gtcggcgtcc
     1681 ggtcgatgtc tcgggttcgt tgtccacggt gataccccaa tcgtcgcgtc gcatgacgag
     1741 ataccggcgg tccgcgcgcg cagcggtgcg ccgcgagaga acccgcatcg cggtagagaa
     1801 gctgtccatc gaccgactcg cgctgtcgga gtcgtgccgg ttgagccaca tcgggacgag
     1861 caccaaaatc cagatagcca cgatgcccag gacgatgaag ctgctcacga gccgctcacg
     1921 ccgtcaggga ggtattcgcc gcaacgccga agccacgtcg tgcgatgggt gctcacgctt
     1981 tgtcacggta gacaggagtg acggatgtga cgagcacatc ggcggtgtgt cggcatatca
     2041 acatgaatgc gacgccgaac gaccacaaga aggcctagcg cggacggcga gatggtggtg
     2101 gtgtcgtcga ctgtgcgcaa tcggcttcaa aacacggcag aaggcagccg attgcgaaca
     2161 ctcggcgcgc aagtgaagcg ggggggctcg caaacacggg tgagccgcga tcgccacgta
     2221 ctaagggtgt aactgctcta gccctggtta ccggctccgg tcagtcgggc ggcgccaccc
     2281 gcagcgagcc atcgcggcag caggccgccc ggcacgtcct cgacggtcag cgcgtacgag
     2341 agatggtcgc gccacccgcc cgcgatatgg atgtagcgct cacgggtgcc ctcgagccgg
     2401 aatcccagct tctcaaccac ccgcctgctc gccgcgttct ctggcctgat gttcgcctcg
     2461 acgcggtgca atccggcgtc cgcgaagcaa tggtcgacga ccatcgccaa ggccgtcggc
     2521 atgatccccc ggcccgcgaa cggcttcgcg atccagtacc cgagatacgc cgagttcgcc
     2581 gacccgcgcg cgatcgcacc gaccgtgacc tgtccggcga agcggtcgtc gacgagcatc
     2641 gcgaacggat aacacaagcc caggcgagcg tcgcggcgca gcgcgcggac cgcgtcgccg
     2701 tacccgcgcc cggcatcaac cggcgcggc
//
LOCUS       DASWVF010000427         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_419184, whole genome shotgun sequence.
ACCESSION   DASWVF010000427 DASWVF010000000
VERSION     DASWVF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419184"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1435)
                     /locus_tag="VGD55_07725"
     CDS             complement(116..1435)
                     /locus_tag="VGD55_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0870272.1"
                     /translation="MKVALDIAVVLALVIIEAVFVATEISLISLRDSQVRGLATKGRR
                     GAAVARLTADPNRFLAGVQIGVTLTSLLSSAYGAVTLSAAAGRELEDLGMTHRSAIVL
                     GFLLVTLSISFVTLVLGELVPKRLALQHAEQAAQLAAPTLDRFASLMRPVIWLLSVWT
                     NIFVRLLGGDPDARRESISEEELRGLVATHESLGRDERRLIDEVFTAGDRQLREVLVP
                     RTEVEFLDAATTVSQALKSVASGPHSRYPVVRGSADDVVGFVHIRDLVMTRADVDPRT
                     VRVGDLARPVKMLPDGKNVLAALSEMRRERQHLAIVVDEYGGTAGIVTLEDLVEEVVG
                     DIRDEYDLDSDGPHRLRGGDVVVDGLLNTDDFAEQAGVTLPEGPYETVAGFLMAELGH
                     LPQLGETVQVGEHRLAVSELDGRRVARVRVSAPPAENEPTEPGHLGE"
     gene            complement(1432..2163)
                     /locus_tag="VGD55_07730"
     CDS             complement(1432..2163)
                     /locus_tag="VGD55_07730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870273.1"
                     /translation="MNRDHAAAWDELGRRDPHWAILSEPGKQGSWDDEGFFATGRTEV
                     DATLKDIGDLLVARDAALDFGCGLGRLTQAMAVHFASVTGVDVARSMVEGAQARNAFP
                     DRVSYVVNTAATLPFDDASFDFVYSNLVLQHVPPPGAERYISELVRVLRPGGVLVFQE
                     LSHRAPTLRNAVLRFVPAGARRIARRTRRGWAATMTMDGVPREKVTALIGAAGGEVID
                     ERVDGAGEPVWKGYRYTVSRPGVVR"
     gene            complement(2194..3408)
                     /locus_tag="VGD55_07735"
     CDS             complement(2194..3408)
                     /locus_tag="VGD55_07735"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0870274.1"
                     /translation="MSKIKVANPVVELDGDEMTRIIWHFIKEQLILPYLDIELEYYDL
                     GIEHRDATGDQVTIDSANAIKKYGVGVKCATITPDEARVEEFGLKKMWKSPNGTVRNI
                     LGGVIFREPIIISNIPRLVPGWTKPIVIGRHAHADQYKATDFVVPGPGTVTITYTPDG
                     DGDPMQFEVAHFAGGGVAMGMYNFDDSIRDFARASLRYGLNRNYPVYLSTKNTILKAY
                     DGRFKDLFAEVFETEFKADFEEAGLTYEHRLIDDMVAAAMKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGRTVEAEAAHGTVTRHFRQHQQGKATSTNPIASIFA
                     WTRGLQHRGKLDGTPEVVGFADALEQVCIETVESGKMTKDLALLIGPDTAWLTTEDFL
                     AALDENLRTKVG"
     gene            complement(3432..4406)
                     /locus_tag="VGD55_07740"
     CDS             complement(3432..4406)
                     /locus_tag="VGD55_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0870275.1"
                     /translation="MSSARKGKVTVVGAGFYGSTTALRLAEYDIFESVVLTDIVEGKP
                     EGIALDINQSRPIEKFETTLIGQTTTVDGDGYGAIEGSRVVVITAGLPRKPGMSRMDL
                     IEVNAKIVRGVAENVAKHAPEAVVIVVSNPLDEMTALTQLATGFTKNRVLGQAGMLDT
                     ARFSNFVAAQLHVPVGAVRTLTLGSHGDTMVPVPSQCTVTIDGQERALTDLLPPDAID
                     ELVTRTRNGGAEIVALLKTGSAYFAPSAAAARMAKAVAEDSGAVMPVCAWVDGEYGIS
                     GVYLGVEAQLGAGGVQRVVETQLTEAELAALHAAAEAVRAKQADVADL"
     gene            complement(4501..>5785)
                     /locus_tag="VGD55_07745"
     CDS             complement(4501..>5785)
                     /locus_tag="VGD55_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0870276.1"
                     /translation="NSDVHKRLKLEQSLRKAIDNDEFVLHYQPKVHVSSGRIVGLEAL
                     LRWEKPGAGVILPDQFVPALEETGLINEVGRWVIRQACKQIASWAKTEIGHMRVAVNV
                     SGRQFLDTDLDGEIGAALLAYGVSADALELELTESSLIPNTERTIATLRKLKARGVQV
                     SIDDFGTGYSCLAYLRKFPIDTLKIDLAFIRNVTTDPADAAMTETIIRMAHSLRMDVI
                     AEGVETAAQLSYLARQGCDQVQGFYFSRALPVLELEELLRAGASLPVHDGTALAPRTT
                     VLLVDDDEVTLSALEALLQQDGYHIVTSLSASNAFELLALHHVQVIVCGGETPLVNRA
                     EFLQRVRDLYPDTLRIVLSASNEPRRVLDAVNRGALYGFYPKPWDDDTLRDNVGAACR
                     HHRRLQDRLQDNALATPERANVHSLEVAALGRVAG"
BASE COUNT         1007 a         1914 c         1838 g         1026 t
ORIGIN      
        1 gcagcgcgcc caggtagttg ccgaggtgaa aggagtcgga tgtcggctgg ataccggaga
       61 ggacgcgcgg ccggccttcc cgaattggct tatctaactg tgggccggtc atggctcatt
      121 ctcccaaatg gccaggctcc gtgggctcgt tttccgctgg cggcgcgctg acccgaaccc
      181 gcgccacccg ccgtccgtct agttccgaca ccgcgagccg gtgctcgccg acctgcacgg
      241 tctctcctaa ttgcggcaga tgacccagtt cggccatgag aaacccggcg acggtctcat
      301 agggaccctc gggcaacgtg accccggcct gctcggcaaa gtcgtcagtg ttcagcaacc
      361 cgtcgacgac cacgtcgccc ccgcgcaggc ggtgcggccc gtcgctgtcg aggtcgtact
      421 cgtcgcgaat gtcgccgacg acttcctcta cgagatcttc gagggtcacg atgcccgcgg
      481 tcccgccgta ttcgtcgacg acgatcgcca ggtgttggcg ttcgcgccgc atctccgaca
      541 gggccgcgag cacgttcttg ccgtccggaa gcattttcac cggccgcgcg aggtcgccga
      601 cccgcaccgt gcgcgggtcg acatcggccc gcgtcatgac gaggtcgcgg atgtgcacga
      661 acccgacgac gtcgtcggcc gacccgcgga cgaccgggta ccgcgagtgc ggccccgacg
      721 cgaccgactt caaggcctgc gaaaccgtcg tcgcggcgtc gaggaattcg acctcagtac
      781 ggggcaccag cacctcacgc aactgccggt cacccgccgt gaaaacctcg tcgatcaggc
      841 gtcgctcgtc gcggccgagc gactcgtggg tcgccacgag accgcgaagt tcttcctcac
      901 tgatggattc gcgacgggcg tcggggtcgc cgccgagcag tctcacgaag atattggtcc
      961 acaccgacag caaccagatg acgggtcgca tcaggcttgc gaaccggtcg agggtaggcg
     1021 ccgcgagttg ggcggcctgc tccgcgtgct gcaacgccag ccgcttcgga accagctcac
     1081 cgagcaccaa cgtgacgaag gagatggaca acgtgacgag gaggaacccg aggacgatcg
     1141 ccgatcgatg cgtcatcccg aggtcttcaa gctcgcgtcc agcggcggcc gacaacgtca
     1201 cggcgccgta cgccgacgag agcaacgagg tgagtgtcac gccaatctga acgccggcaa
     1261 ggaacctgtt cgggtcggcg gtcaaccgtg ccacggcggc gccacgccgg cccttggtag
     1321 cgaggccacg cacctggctg tcgcgcagcg agatgagcga gatctcggtg gcgacgaaga
     1381 cggcctcgat gatcacaagc gccagcacga cggcgatgtc gagcgcgacc ttcatcgcac
     1441 gactcccggg cgcgagacgg tatagcggta gcccttccac accggctcgc cggcgccgtc
     1501 gacgcgttcg tcgatgactt cgccgcctgc tgcgccgatc aatgcggtga ctttctcgcg
     1561 tggcacgcca tccatcgtca tcgtggccgc ccatccgcgc cgagttcgcc tggcgatgcg
     1621 ccgagcgccc gccggcacga agcgaagcac cgcgttgcgc agcgtcggcg cccgatgcga
     1681 gagctcctgg aagaccagca caccgcccgg gcgcagcacc cgcaccaact cggaaatgta
     1741 gcgttcggca cccggcggtg ggacgtgctg cagaacaagg ttcgagtaca cgaaatcgaa
     1801 cgacgcatca tcgaacggca acgttgcggc cgtgttcacc acataggaga cgcgatcagg
     1861 aaacgcgttg cgagcttgcg cgccctcgac catcgaccgc gcaacatcga ccccggtcac
     1921 ggaagcgaaa tgcacggcca tcgcctgggt caggcgccca aggccgcagc cgaaatcgag
     1981 agcggcgtcg cgcgcgacga gcaggtcacc gatgtctttc agtgtcgcgt cgacctctgt
     2041 gcggccggtc gcgaagaacc cttcgtcgtc ccagctgccc tgcttgcctg gttctgacag
     2101 gatcgcccag tgcggatcgc gccgacctaa ttcgtcccac gccgcggcgt gatcacggtt
     2161 catgcggcaa ggttagcggg cgccggacgg cgatcagccg accttggtac gcaagttctc
     2221 gtcgagggcg gcgaggaaat cctcggtcgt gagccacgcc gtgtcaggcc cgatcagcaa
     2281 tgcgaggtcc ttggtcatct tgccgctctc gacggtttcg atgcagacct gctcgagcgc
     2341 atcggcgaag ccgaccactt cgggggtgcc atcgagcttg ccccgatgtt ggagcccgcg
     2401 ggtccaggcg aagatcgatg caatcgggtt ggtcgacgtc gccttaccct gctggtgctg
     2461 ccggaaatgg cgggtgaccg tgccgtgcgc cgcttctgcc tcgaccgtgc ggccgtcggg
     2521 agtcatcaat accgacgtca taaggccgag cgatccgaac ccttgggcca ctgtgtctga
     2581 ctgcacgtcg ccgtcgtagt tcttgcacgc ccacacgtag ccgccctccc acttcatggc
     2641 cgccgcgacc atgtcgtcga tgagccggtg ctcatacgtc aggcccgctt cttcaaagtc
     2701 ggccttgaac tcggtctcga aaacctcggc gaacagatcc ttgaagcgac cgtcgtacgc
     2761 cttcaggatc gtgttcttcg tcgagagata aaccgggtag ttgcggttga gtccgtagcg
     2821 cagcgacgcc cgcgcgaagt ctcgaatcga gtcgtcgaag ttgtacatgc ccatcgcgac
     2881 gccgccgccg gcgaagtgtg cgacttcgaa ctgcatcggg tcgccgtcgc cgtcgggggt
     2941 gtacgtgatc gtgacggtgc ccgggccggg cacgacgaag tcggtggcct tgtattgatc
     3001 ggcgtgggcg tggcggccga tgacgatggg cttggtccag cccggcacca ggcgcggaat
     3061 gttgctgatg atgatgggct cacggaagat gacgccgccg aggatgttgc gaactgtgcc
     3121 gttcggcgac ttccacatct tcttcaagcc gaactcttcg acgcgcgcct cgtcgggcgt
     3181 gatcgtggcg cacttcaccc cgacgccgta cttcttgatg gcgttggccg aatcgatggt
     3241 cacctgatcg ccggttgcgt cgcgatgctc gatgccgagg tcgtagtact cgagctcgat
     3301 gtcgaggtag ggcagaatca actgctcctt gatgaagtgc caaatgatgc gtgtcatctc
     3361 gtcgccgtcg agctcgacga cgggattggc gaccttgatc ttcgacatct gttctccact
     3421 acctcgaatg cttacagatc tgcgacgtcg gcttgcttgg cgcgcaccgc ttcggcagcc
     3481 gcgtgcaacg ccgccaactc ggcctcggtg agttgggtct cgaccactcg ctgcacccca
     3541 ccggctccga gttgggcctc gacaccgagg tagacgccgc tgatgccgta ttcgccgtcg
     3601 acccacgcac acaccggcat gacagcgccg gagtcctcgg cgacggcctt ggccattctt
     3661 gcggcggcgg ccgacggtgc gaagtaggcc gagcccgtct tcaagagcgc gacaatctcg
     3721 gcaccgccgt tgcgcgtgcg ggtgacgagc tcgtcgatcg cgtcgggcgg cagcaggtcg
     3781 gtaagtgcgc gttcctggcc atcgatcgtc accgtgcact gagagggcac cggcaccatc
     3841 gtgtcgccgt gcgagccaag ggtcagcgtg cgaacggcac cgacgggcac gtgcagttgg
     3901 gcggccacga agttgctgaa ccgagccgta tcgagcatgc cagcctggcc gagcacgcgg
     3961 ttcttcgtga atccggtggc caactgggtc agcgccgtca tctcgtcgag ggggttcgac
     4021 acaacaatca cgacagcctc gggcgcgtgc ttcgcgacgt tttcggcgac cccgcgaacg
     4081 atcttcgcgt tcacctcgat cagatccatg cggctcatgc cgggcttgcg tggcaggcct
     4141 gctgtgatga cgacgacccg ggagccttcg atcgcgccgt acccatcgcc gtccaccgtc
     4201 gtcgtctgac cgatcaacgt cgtctcgaat ttctcgatcg gccgcgactg attgatgtcg
     4261 agagcgatgc cttcgggctt tccctcgacg atgtcagtga ggacgacgga ttcgaagatg
     4321 tcgtattcgg ctaaccgcaa cgcggtggtc gagccgtaaa aaccggcacc gaccacggtg
     4381 actttgcctt tgcgcgcact gctcatcgag atcatgctcc tggccagcga gggagaggct
     4441 ccatgctatt gccccaacca ccccccgccg ggcgggggtc agctgcccag atgctttcgg
     4501 ctagcccgcc actcggccca gagcagccac ctcgaggctg tggacgttcg ctcgctcagg
     4561 cgtcgctagt gcgttgtctt gcaagcggtc ctgcaatcga cggtggtgcc ggcacgctgc
     4621 accaacgttg tcgcgcagcg tgtcgtcgtc ccaaggcttg ggatagaacc cgtacagcgc
     4681 accgcggttg accgcgtcga gtacccggcg cggctcattg ctcgccgata agacgatgcg
     4741 cagcgtgtcg gggtacagat ctcgcacccg ctgaagaaac tcagcccggt tcaccaacgg
     4801 cgtctccccg ccgcacacga tcacctgcac atggtgcagc gcgagcagct cgaacgcgtt
     4861 cgacgccgaa agcgacgtca cgatgtgata gccgtcctgc tggagcagtg cttcgagagc
     4921 agacagcgtg acctcgtcgt catcgacgag cagcaccgtc gtgcggggtg cgagcgccgt
     4981 gccatcgtgc accggcagcg acgcgcccgc gcggagcagt tcctcgagct cgagaacggg
     5041 cagtgctcgg ctgaagtaga agccctgaac ctggtcgcac ccctggcgcg ccagatacga
     5101 gagctgcgcc gcggtctcga cgccttcggc gatgacatcc atccgcaggc tgtgcgccat
     5161 ccggatgatg gtctctgtca tcgcagcgtc ggcggggtcg gtagtgacgt ttctgatgaa
     5221 ggcaaggtcg attttgaggg tatcgatcgg gaacttgcgc aggtaggcca gacacgagta
     5281 gccggtgccg aagtcatcga tcgacacctg cacgccgcgc gctttcaact tccgcaacgt
     5341 cgcgatggtc cgctcggtgt tcggaatcag ggagctctcg gtcaactcga gttcgagcgc
     5401 atcggcagag actccgtatg ccagaagagc cgcgccgatc tcgccgtcaa ggtcggtgtc
     5461 gaggaactgc cggccagaca cattgacagc caccctcatg tgcccgatct cagtcttggc
     5521 ccacgaggcg atttgcttgc acgcctgccg gatcacccat cggcccacct cgttgataag
     5581 cccggtctcc tccaacgcgg gaacgaactg gtcaggcagg atcaccccag cccctggctt
     5641 ttcccaacgc agcaatgctt ccaagccgac gatccggccg ctgctcacgt gcaccttcgg
     5701 ctggtagtgc aagacgaact cgtcgttgtc gatggccttg cgcaacgact gctcaagctt
     5761 cagtcgcttg tgcacgtcgg agttc
//
LOCUS       DASWVF010000428         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_189880, whole genome shotgun sequence.
ACCESSION   DASWVF010000428 DASWVF010000000
VERSION     DASWVF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189880"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VGD55_07750"
     CDS             complement(<1..1344)
                     /locus_tag="VGD55_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0870277.1"
                     /translation="MLERLLVAPGRRERVTHVEIVPARAAVFAAWPSWLHPDLIEAYA
                     RLGIGEPWAHQVEAAEHAHAGRHVVVATGTASGKSLAYQLPVLDRMLNGPAGTTALYL
                     SPTKALAADQLRALGELELSWVRASTYDGDTPYEERAWVRAHANLVLANPDLVHRSML
                     PAHRQWASFFRGLSFVVIDECHGYRGVFGSHVAQVIRRLRRVAARYGAFPVFVLASAT
                     VSEPATSASRLIGLDVEAVTDDASPRGASRFVLWEPPLTHFGGGAQFGNGGQSGDSAP
                     VRRSAVAETADLLTDLVADGVRTLAFVRSRRGAEAVALAARRALDEVSPNLGRRVAAY
                     RAGYLPEERRALERQLRSGSIVGLAATTALELGVDVSGLDAVLLAGFPGTRSSMWQQA
                     GRAGRDGQESIAVMTARDDPLDTYLVHHPEALFGRPVEAAVFDPDNPYVLGPHLCA"
     gene            1653..1994
                     /locus_tag="VGD55_07755"
     CDS             1653..1994
                     /locus_tag="VGD55_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEY0870278.1"
                     /translation="MDLSLSTRAVGDHTIVVVGGEIDVYTAPKLREQLIDLVSAGSYH
                     IVVDMENVEFLDSTGLGVLVGGLKRVRAHDGSLRLVCTQERILKIFRITGLTKVFPIH
                     TSVDEAVAATD"
     gene            2019..>2403
                     /locus_tag="VGD55_07760"
     CDS             2019..>2403
                     /locus_tag="VGD55_07760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870279.1"
                     /translation="MTVSFTPLHEHVRTARFIAASVARRAGFTDGVVDEIRLAVSEAC
                     SLAVRFHQAHAPGAPVEVRLIESSKQFSVEVADAVGRAAPESDADWTFDLSDEDAGAA
                     GLSEPVDLGGHERIGLAVISGLVDDM"
BASE COUNT          403 a          814 c          821 g          365 t
ORIGIN      
        1 cgcgcacagg tgtggcccga gcacgtacgg gttgtcggga tcgaacacgg ccgcttcgac
       61 cgggcgtccg aacagcgcct cgggatggtg caccagatag gtgtcgagcg gatcgtcgcg
      121 ggccgtcatt accgcgatcg actcctgccc gtcgcgaccg gccctacccg cctgctgcca
      181 catcgacgac cgggtgccgg ggaatccggc gagcagcacc gcgtcgagcc ccgacacgtc
      241 gacgcccagt tcgagtgcag tcgtggccgc caggcccacg atcgaacccg agcgcagttg
      301 gcgttcgagc gcccggcgtt cttcgggcag gtagccggcg cggtaggccg cgacgcggcg
      361 acccaggttc ggcgacacct cgtcaagcgc gcgacgggca gcgagtgcga cggcctcggc
      421 accgcggcgc gatcggacga acgccagcgt gcggacgccg tcggcgacca ggtcggtaag
      481 tagatcggcg gtctcggcca ccgccgagcg acgcaccggc gcgctgtcac cggactgccc
      541 gccattgccg aactgggcgc cgccaccgaa gtgcgtaaga ggcggttccc agagcacgaa
      601 gcgcgaagcg ccccgaggcg acgcgtcgtc ggtgactgcc tcgacgtcca agccgatgag
      661 cctggacgcc gatgtggctg gctccgacac cgtcgccgac gcgagcacga acacaggaaa
      721 tgccccgtac cgcgcggcca cccttcgaag ccggcgaatc acctgcgcga cgtgcgagcc
      781 gaacactccc cggtacccgt ggcattcgtc gatgacgacg aacgacaggc cgcggaagaa
      841 cgaagcccat tgccggtgcg ccggcagcat cgagcgatgc accagatcgg ggttggcgag
      901 caccagattg gcgtgcgccc gcacccaggc acgctcttca tagggggtgt caccgtcgta
      961 ggtcgaggct cgcacccagg agagctcgag ctcgcccagc gcccgcaact ggtcggcggc
     1021 cagtgcctta gtcggcgata agtagagagc cgttgtgcca gcaggcccgt tgagcatccg
     1081 gtcgagcacc ggcagttgat aggccagcga cttgccagag gcagtgccgg tggcaaccac
     1141 gacatggcga cccgcgtggg cgtgctcggc cgcctcgacc tgatgcgccc atggttcgcc
     1201 gatgccaagc cgggcgtagg cctcgatcag gtccggatgc agccaactcg gccaggcggc
     1261 gaacacagcg gcccgcgccg gcacgatctc gacgtgggtg acgcgttcgc ggcgccccgg
     1321 cgcgaccagc aatcgctcaa gcactcgctc gctcgatcgc atcggcccgg ttgggcgcga
     1381 aggagaggtc aggctggagc ctgcgagcaa ccggggcgac cccggcggcc cgatcgatgc
     1441 gttcgacgac aggctttgcg cggacactgc ggccagtctg ccatcggcgg gcgacggaat
     1501 cggccaccga cgagcccgga cggcacgcaa tgacacccac tgagaagagc gtggttagat
     1561 gcgtggttac ggggtacccg gcttttcaac atagccgcgt gcccggcgtc cgagagtgct
     1621 gcccgtgtgg cgtcctgagc tggaggaacg cagtggatct gtctttgtcg acccgagccg
     1681 ttggtgacca caccatcgtc gtcgttggcg gcgagatcga cgtctacacc gcaccgaagt
     1741 tgcgcgagca actcatcgac ctcgtgtctg ccggcagtta ccacatcgtc gtcgatatgg
     1801 agaacgtcga gttcctcgac tcgaccggcc tcggcgtcct cgtcggtgga ttgaagcggg
     1861 tccgtgccca cgacgggtcg ttgcgcctcg tgtgtaccca ggagcgcatc ctcaagatct
     1921 ttcgaatcac cggcctgacg aaggtcttcc ccatccacac ctcggtcgac gaggccgtcg
     1981 ccgcaacgga ctgagcggcg ccctacctcg tgccaaccgt gacggtctcg ttcactcctt
     2041 tacatgagca tgtacgaacc gctcgcttca tcgcggcgtc cgtggcccgg cgcgcggggt
     2101 tcaccgacgg cgtcgtcgac gagatccggc ttgcggtcag cgaggcctgt tccttggccg
     2161 tgcgctttca tcaggcacac gcccccgggg caccggtcga ggtgcgcctg atcgaatcga
     2221 gcaagcagtt ctcggtcgag gtcgccgatg ctgtcggtcg ggcggcccct gaatcggatg
     2281 ccgactggac gttcgatctc agtgatgagg acgccggggc agcgggcctc agcgagccag
     2341 ttgacctcgg ggggcatgag cgcatcgggc tcgccgtcat cagcgggttg gtcgacgaca
     2401 tgt
//
LOCUS       DASWVF010000429         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_87745, whole genome shotgun sequence.
ACCESSION   DASWVF010000429 DASWVF010000000
VERSION     DASWVF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87745"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VGD55_07765"
     CDS             complement(<1..1423)
                     /locus_tag="VGD55_07765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEY0870280.1"
                     /translation="MGKGAPAAALMGQVRSAIRAYAVSGLSPTEVLSSLDRLFDTLIE
                     DRVVTAVVGMITPSTGRVVLSNAGHPPPLVVRADGSATFCPMQRTLLIAAGLSGSLRP
                     RDEVVLERGDSLIMYSDGLIERRGEVITHGMERLADTATVIARAGWPDHPAVTFASML
                     SVEERTDDIVVLCLKYTGSSVGRISASPVGTSREGMSTLHLEPVVESTPVARHWVAAH
                     LRDLPAEVTSCAALLTSELVTNAVLHAATPMSITLHTLPDRIRVDVADGNPAFPSIKD
                     YGKEAATGRGLTLFNTLASNWGVQAVDGGKIVWFELPVDFPSTPVSVSDGSFRFDLTG
                     ATRAGLGAPEDVDVVSVRLLGIPVALLQKSSEEYEALFRELRLMKERAASSPDAPELP
                     ERLGVLVSQIGTRFNGLGPGMDDLWQTAVDNNVEFFDWTLDLAQSAVVAVEFYDTLLD
                     EADEFGLTQRILTLPASPSSVAVR"
BASE COUNT          268 a          501 c          507 g          245 t
ORIGIN      
        1 ggcggacggc cacgctcgac ggtgatgcgg gcagcgtcag gatccgctgc gtcagcccga
       61 attcgtcggc ctcgtccagc agggtgtcgt agaactcgac ggcgacgacc gcggactgcg
      121 ccaggtcgag tgtccagtcg aagaattcga cgttgttgtc gacggcggtc tgccagaggt
      181 cgtccatgcc cgggcccaac ccgttgaaac gcgtgccgat ctgggagacc aggacgccga
      241 ggcgttcggg cagttccggc gcgtcgggtg agctggcggc tcgttccttc atcaaccgga
      301 gctcacggaa cagggcctcg tattcctcgc tcgacttctg cagcagcgca accggaatgc
      361 ccaggagccg cactgagacc acgtccacgt cttcgggcgc gccgaggccg gctcgcgtcg
      421 caccggtcag gtcgaagcgg aagctgccgt cagagacact cacgggggtc gacgggaagt
      481 cgaccggtag ctcgaaccag acgatcttgc cgccgtcgac cgcctgcacc ccccagttcg
      541 aagccaatgt gttgaacagc gtgaggccgc gaccggtggc cgcttccttc ccatagtcct
      601 tgatcgacgg aaaggcggga ttcccgtcgg ccacgtcgac gcggatgcgg tctggaaggg
      661 tgtgcagcgt gatgctcatg ggagtcgccg cgtgcaggac ggcgttcgtc accaactcgg
      721 acgtcaacag cgcggcgcaa ctggtcactt cggcgggaag gtcgcgcaga tgtgccgcga
      781 cccagtgtcg tgccacaggg gtgctctcca cgaccggttc gagatgcaac gtcgacatgc
      841 cttcccgtga cgtacccact ggcgaggcgg atatgcgccc gacggacgaa ccggtgtatt
      901 tgaggcagag caccactatg tcgtccgtgc gttcctccac gctcagcatg gaagcgaagg
      961 tgaccgcggg atggtccggc caaccggctc gcgcaatgac tgttgccgta tccgccaggc
     1021 gctccatccc gtgcgtgatg acctcgccgc ggcgttcgat caagccgtcc gaatacatga
     1081 tcaacgagtc gccgcgttcg agaacgactt cgtcgcgcgg ccggagtgag ccgctcaggc
     1141 ccgccgcgat cagcagtgtc cgttgcatcg gacagaacgt cgcgctgccg tctgcgcgta
     1201 cgaccagcgg gggaggatgg ccggcgttgc tcaagacgac gcgtcccgtc gagggagtga
     1261 tcatgccgac caccgcggtc acgacacggt cttcgatgag cgtgtcgaag agccggtcca
     1321 acgacgagag gacctccgtc ggcgacaggc cggagaccgc gtaggcgcgg atggcgctgc
     1381 gaacctggcc catcagggcg gccgcgggcg cacccttacc catgacatcc ccgacgctga
     1441 agaacacttc gtcgtcaccg agctcgaaca cgtcgtacca gtcgcccccg gcgtaggtcc
     1501 cggcgactcc cgctcggtac t
//
LOCUS       DASWVF010000430         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_11579, whole genome shotgun sequence.
ACCESSION   DASWVF010000430 DASWVF010000000
VERSION     DASWVF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11579"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1305)
                     /locus_tag="VGD55_07770"
     CDS             complement(121..1305)
                     /locus_tag="VGD55_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870281.1"
                     /translation="MALDAVFRDHWGRVVATLVRVLGDIELAEDAAQEAFAIAAERWP
                     RDGEPENPVGWLILTARNRAIDHIRRQRVLAQKTKLLAYELEIGSEATMREASTFPDE
                     RLELIFTCCHPALALEAQVALTLRTLGGLTTEEIALAFLVPFETMSKRMTRAKHKIRD
                     AGIPFAVPPDDQLPDRLAAVLAVVYLVFNQGWGGGRVDLAGGAIELGRALADLMPDEG
                     EVLALLALMLLHDARRAARLRDGAIVLLDEQDRALWDERQIDEGRQLLQRVLARGDTG
                     PYAVQAAIADLHLHQPRDWGQIAALYGRLAVQTGSPVVELNRAIAVAEIDGPSAGLAV
                     LDALELDHYRYFHSARAELLRRAGRGNEARHAYRRALDLAQTDSERRHLQDRLAELSS
                     SD"
     gene            complement(1295..1633)
                     /locus_tag="VGD55_07775"
     CDS             complement(1295..1633)
                     /locus_tag="VGD55_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0870282.1"
                     /translation="MQYALLAYGSQSSQEAGRPMLPAIADVLARPYVTGWVRLHADES
                     ATTVRNAEGGSLLTDGPFIESKEYLAGLIMIEAANLDEALAIAQELQQTRTSGAIEVR
                     PVVEGLFSGA"
     gene            1815..>2067
                     /locus_tag="VGD55_07780"
     CDS             1815..>2067
                     /locus_tag="VGD55_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870283.1"
                     /translation="MTNTRPPSTASTRQLRWVLGLTSTAYFMVVLDALVVTTALPRMQ
                     RDLHTNLASLQWTVNSYGIAFAAGIITAAALGDRFGRRRV"
BASE COUNT          356 a          679 c          675 g          357 t
ORIGIN      
        1 gaagtcgcgc ggcggtgcgg cctgggcaca gccgccaacc tgcgcctgca ttttcgccgg
       61 ttcgtcggca cgacacccac cgcctaccgc aaggcattcc cgggctgaat gactgtgccg
      121 ttagtcggac gaactcagtt cggccagccg gtcctgaagg tgtcggcgct cgctgtcggt
      181 ctgcgcaaga tcgagcgctc gcctgtaggc gtgccgggcc tcgttgccac gcccggctcg
      241 gcgaagcagc tcggcgcggg ccgagtggaa gtagcggtag tggtcgagtt cgagggcatc
      301 cagcacggca aggcctgccg atggtccgtc gatttcggcg acggcgatgg ctcggttcaa
      361 ctcgacgaca ggggaaccgg tctggacggc gagcctgccg tagagagcgg caatctgccc
      421 ccagtcgcgc ggctggtgca agtgcagatc ggcaatggct gcctgcaccg catagggccc
      481 tgtgtcaccg cgggcgagta ctcgctggag aagctgcctg ccttcgtcga tctgacgttc
      541 gtcccacagc gcacgatcct gctcgtcgag caacacaatc gcaccgtcgc gcaaccgcgc
      601 ggcccgacgt gcgtcgtgca gcagcatgag ggcgagcagc gcaagcacct cgccctcgtc
      661 cggcatcagg tcggcgagcg cacggccgag ctcgatcgcc ccgccggcga ggtcgacgcg
      721 accgcctccc caaccctggt tgaaaacgag gtacacgaca gcaagaactg ccgccagcct
      781 gtcgggtagc tggtcgtctg gtggcaccgc gaatgggata cctgcgtcac ggatcttgtg
      841 cttggcgcgc gtcatgcgtt tgctcatcgt ctcgaacggc acgagaaagg cgagcgcgat
      901 ctcctcggtg gtcaagccac caagggtgcg cagggtgagc gcgacctgtg cttccagggc
      961 gagtgcgggg tggcagcagg tgaagatcaa ttcgaggcgc tcgtccggaa aggtggatgc
     1021 ctcacgcatc gttgcctccg atccgatctc gagttcgtag gcgagcagct tcgttttctg
     1081 tgccaaaaca cgttgccgac ggatgtggtc gatcgcgcgg ttgcgtgcgg tcaggatcaa
     1141 ccaaccgacg gggttctcag gttcgccgtc gcgcggccat cgctcagcag caatcgcgaa
     1201 ggcttcctgc gccgcatcct cagcgagctc gatgtcgccg agaacacgaa cgagggtcgc
     1261 gaccacccga ccccagtggt cgcggaagac ggcgtcaagc gccactgaag agaccttcga
     1321 cgacgggccg cacctcgatc gcgccactgg tcctcgtctg ctgaagctcc tgcgcgatgg
     1381 ctagtgcttc atcgaggttg gcggcctcga tcatgatcag gccggcgaga tactccttgc
     1441 tctcgatgaa gggcccatcg gtcaggagcg accctccctc ggcgttgcgt acggtcgtcg
     1501 ccgactcgtc agcgtgcaga cgaacccagc cggtcacgta gggacgcgcc agcacgtcgg
     1561 cgatcgccgg gagcatcggg cgcccggcct cctggctgct ttggctgcca taagcgagca
     1621 atgcgtactg catcggccct ccagtcgctg atcggtcccg gtttcgagta tctgacgaac
     1681 gacatcgtcg ccgacggaca tggcaggcga cgagatgcga gaaatgtttg atccagctga
     1741 tgtccgtgag gccgcgagcc gttcgtcatt gctccagaac gcacccgacg aggtgataaa
     1801 cgctggagga gccgatgacc aacacccgac cgccgtccac cgcaagcacg aggcagttgc
     1861 gctgggtact cggcctcacc tcgactgcgt acttcatggt ggtgctcgac gcgctcgtcg
     1921 tgaccaccgc gctgccacgc atgcagcgcg acttgcacac caatctcgcg tctctgcaat
     1981 ggaccgtcaa ctcctacggc atcgccttcg cggccggcat catcacggcc gcagcgctcg
     2041 gtgatcgatt cggccgccgg cgggtgt
//
LOCUS       DASWVF010000431         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_470207, whole genome shotgun sequence.
ACCESSION   DASWVF010000431 DASWVF010000000
VERSION     DASWVF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470207"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGD55_07785"
     CDS             complement(<1..601)
                     /locus_tag="VGD55_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870284.1"
                     /translation="MRRRLLVSTLVVALVSVILLGIPLGIVTTKLIRDEAQKRINREA
                     TQIATALDNSASTPPSGSSLDASATAVTMGILKQFASDDRYVSVVEAGQTLATGTKDE
                     TVTVIASASSTNNELASVWIGVTALAIVSLAAAVELARRQAKRLGGPLEDLAIGAERL
                     GAGDPRPRNHRYGVPELDKVAEVLDRSAERIGQLLGAERA"
     gene            complement(608..1276)
                     /locus_tag="VGD55_07790"
     CDS             complement(608..1276)
                     /locus_tag="VGD55_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870285.1"
                     /translation="MTSVLLAEDDVSISEPLARALRREGYDVEVKSEGTAVLQRVLSG
                     GVDLVVLDLGLPTMDGLEVCRRMRAEGRSVPVLVLTARAEEVDLVVGLDAGADDYVTK
                     PFRLAELLARVRALLRRGAPEVPDVNGVRINAESRRAWLGDEELSLTAKEFDLLLALV
                     LGAGKVLTREQLMRDVWGMSRGASSKTLDMHVSWLRRKLGDDAASPRLITTIRGLGFR
                     FERG"
     gene            complement(1301..>1560)
                     /locus_tag="VGD55_07795"
     CDS             complement(1301..>1560)
                     /locus_tag="VGD55_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0870286.1"
                     /translation="ADAAVNAVHANRLAAMADGDERAALIDKLRAEYEADIDIMRLAA
                     ELVVDDIVEPEDLRANLIRRLGAASNKERTFSRRRHGITPV"
BASE COUNT          277 a          541 c          498 g          244 t
ORIGIN      
        1 gcgcacgctc ggcgccgagc agctgaccga tgcgttcggc actgcggtca agcacttcgg
       61 ccaccttgtc gagttcaggc acgccgtaac gatggttacg gggtcgcggg tcacccgcac
      121 cgagccgctc ggcgccgatc gcgaggtcct ccagtggccc accgagccgc ttcgcctgcc
      181 gccgcgccag ctcgaccgca gccgcgaggg acacgatcgc cagggccgtg acgccgatcc
      241 agaccgacgc aagttcgttg tttgtcgacg acgccgacgc aatcaccgtc accgtctcgt
      301 ccttcgtccc ggtcgcgagc gtctgacccg cttcgacgac ggacacgtag cggtcgtcgc
      361 tggcgaattg cttcagaatg cccatcgtta cggcggtcgc agaagcatca agcgatgagc
      421 cggacggcgg ggtgctcgcg ctgttgtcca acgcggtggc gatctgcgtc gcctcgcgat
      481 tgatacgctt ctgcgcctcg tcgcggatca acttggtcgt gacaataccg agcggaattc
      541 ccagcagaat aacggaaact agtgcaacaa ccagcgtaga gacgagcaac cgccttcgca
      601 cgctggatca gccacgctcg aagcggaacc ccaagccacg gatcgtcgtg atgagccggg
      661 gtgaggcagc gtcgtcaccg agcttgcgac gcagccacga aacgtgcatg tcgagagtct
      721 tcgacgacgc cccgcgactc atgccccaca catcgcgcat caactgctcc cgggtgagca
      781 ccttgcctgc gccgagaacg agggcaagca gcaggtcgaa ctccttagcg gtcaagctga
      841 gttcctcgtc gcccagccac gcgcgacgcg attccgcgtt gatgcgcacg ccgttgacgt
      901 cgggcacctc aggagcgccg cggcgcagga gcgcccgcac ccgggccagc agctcggcga
      961 gccggaacgg cttggtcacg tagtcgtcag cgcccgcgtc aaggccgaca acgaggtcga
     1021 cctcctcagc ccgggcggtc aagacgagca ccggcaccga gcgaccttcg gcgcgcatcc
     1081 ggcggcacac ctcgagaccg tccatcgtgg gaagcccgag atcgagcacc acaagatcga
     1141 ccccgccgct gagcacgcgc tgcagcactg cagtgccttc gctcttgacc tcgacgtcgt
     1201 acccctcacg gcgcagggcc cgggcgagcg gttcggaaat cgagacatca tcctcggcga
     1261 gcaagacgga ggtcatgaac gaatcgtatg ggtacgcgag tcaaaccggg gtaatcccgt
     1321 ggcgccttcg cgaaaacgtc cgctccttgt tcgaggctgc gccgagccgg cggatcaggt
     1381 tggcgcgcag atcctcgggt tcgacgatgt cgtcgacgac cagttcggcg gccagccgca
     1441 tgatgtcgat gtcggcctcg tactcggccc gcagcttgtc gatcagcgcc gctcgctcgt
     1501 cgccgtcggc catcgcggcg agccggttgg cgtgtacggc gttgaccgct gcgtcggccc
//
LOCUS       DASWVF010000432         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_11606, whole genome shotgun sequence.
ACCESSION   DASWVF010000432 DASWVF010000000
VERSION     DASWVF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11606"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGD55_07800"
     CDS             complement(<1..269)
                     /locus_tag="VGD55_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870287.1"
                     /translation="MSPAGRRPGKTDTRQVILDAATVCFAESGYDGTTIRAIATQAGV
                     DPALVHHFCGAKGELFAAAVDFPFSPRAVLPALLEGAGDDAGERIV"
     gene            complement(279..1016)
                     /locus_tag="VGD55_07805"
     CDS             complement(279..1016)
                     /locus_tag="VGD55_07805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870288.1"
                     /translation="MSRSRFDAVAQEYDAGRPGYPDDLYDAIEELSGSFLAGALLADV
                     GAGTGISTRGLLERGARVVAVDHGTTMLATLRGRTPATAAVLADANELPFRDGSLDLV
                     TFAQSWHWVDLSRAPREVLRVLRPGGALAVWWNTADEGGEQWLHDHRARLRAVGQHAV
                     GARFDAAWRSIPSAFEHLDVETTNIKWDRTVPMGVILDEVRSKSYIAELGAAGADEFV
                     ERERAMLPPGLLRQPFVTQLAVVRMPA"
     gene            complement(1030..1848)
                     /locus_tag="VGD55_07810"
     CDS             complement(1030..1848)
                     /locus_tag="VGD55_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY0870289.1"
                     /translation="MGGALEGAGASPAVTLSTSSVWPESTAVAFEVAARLGYDGVELM
                     VSGDPVSQDLDTLRRLVDYHQMPVVSVHSPCLLISQRVWGTEPWAKLMRSQIAAERLG
                     AKVVVTHPPFRWQRGYAKGFTAGIVAMHEETDIVFAVENMFPQRGPSGLELGVYSPSW
                     SLLDADYPNVTLDVSHAAVARADPLELLQGFGDRLAHVHLADGTGSRNDEHLIPGRGN
                     QPCAELLRQLSAQRYSGAVVLEVNTRRAASHEDRVTELAEALAFTRQALAATAA"
     gene            1936..2832
                     /locus_tag="VGD55_07815"
     CDS             1936..2832
                     /locus_tag="VGD55_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870290.1"
                     /translation="MPRKPSKSSPPSRPAESSRAATRPAGKTKTAAAEPAHPIIPTQA
                     GASASERIPLPEKGLDFAREWVEFVDPADPEQIFRCDLTWLTSSWGCIFGNGCHGIVP
                     GRAADGCCTHGAFYSGRADEKRVREFAEQLTPETWQFYKKGQKGFTELEDGKRRTRRV
                     DGACIFLNRPGFEGGVGCSLHSLALTIGRHPLETKPDVCWQLPIRRTFDWIDRPDETK
                     VLIVSIGEYDRRGWGSGGHDLNWWCTASPEAHGARDRVYISYGPELTELMGPQAYATL
                     VEHCERRLASKTPVAPHPADPV"
     gene            2923..>3539
                     /locus_tag="VGD55_07820"
     CDS             2923..>3539
                     /locus_tag="VGD55_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0870291.1"
                     /translation="MPKTSTPGQVKLSFRCGECGWESLKWVGRCGECQAWGTVEQLGL
                     PRLRSVAATSVTAAARPIGDIDAEAARSRPTGVDELDRVLGGGLVPGVVVLLAGEPGV
                     GKSTLVLDVASKFAGLDTPDFAGARTLYITGEESTAQVRLRAGRIKALSPHLFLAAET
                     DIGAVLKHVDDVDPALLIVDSVQTMAIADVDGSAGGVTQVRQVAAA"
BASE COUNT          619 a         1206 c         1144 g          570 t
ORIGIN      
        1 acgatccgtt cgccggcgtc atcgcccgca ccttcgagga gcgccggtag aaccgcgcgc
       61 ggcgagaacg gaaaatcgac ggcggctgcg aacagctcgc ccttcgcacc acagaagtgg
      121 tgcaccagtg cgggatcgac gccggcttgg gtggcgatcg ccctgatcgt ggtgccgtcg
      181 taaccggatt cggcgaagca gacggtggcg gcgtcgagga tcacctgacg ggtatcggtc
      241 tttccgggac ggcggccagc gggcgacatc gtcggccctc aggcaggcat gcggacgacg
      301 gcaagctgcg tcacgaacgg ctgcctgagg agccccggcg gcaacatcgc gcgctcgcgc
      361 tcgacgaact cgtcggcgcc cgccgcaccg agctcggcga tgtaggactt cgaacgcact
      421 tcgtcgagga tcacgcccat cgggacggtt cgatcccact tgatgtttgt cgtctcgacg
      481 tcgaggtgct cgaaggccga agggatcgat cgccacgcag cgtcgaatcg ggcaccaacc
      541 gcgtgctggc cgacggcgcg caaccgggcg cgatgatcgt gcagccactg ctcgccaccc
      601 tcgtcggcgg tgttccacca gacggcgagc gcgccgccgg gtcgcagcac ccgcagcact
      661 tcgcgcggcg cacgcgaaag atcgacccag tgccacgatt gcgcgaacgt cacgaggtcg
      721 agcgagccgt cacgaaacgg cagctcgttt gcatcggcca gcaccgctgc tgttgcgggc
      781 gtgcgacctc gcaacgtggc gagcatcgtg gttccgtgat cgacggctac gacgcgcgca
      841 ccgcgttcaa gcagcccacg ggttgagatg ccggtgccgg cgccgacatc ggcaagcaag
      901 gcgccagcga gaaagctgcc cgacaactcc tcgatcgcgt cgtataggtc gtcgggatat
      961 ccgggccggc ccgcgtcgta ctcctgcgcg acggcatcga accgcgaccg gctcacgccg
     1021 ctctcgcatt catgccgctg tcgccgcgag tgcctggcgg gtaaaggcga gtgcctcggc
     1081 aagctcggtc actctgtcct cgtgcgaagc tgccctgcgc gtgttgacct cgagcacgac
     1141 tgcaccgctg taccgctgcg ccgacaactg ccgcagtagc tcggcacatg gctggtttcc
     1201 gcggccggga atgaggtgtt cgtcatttcg tgaaccggtg ccgtccgcca ggtgcacgtg
     1261 ggcgagccgg tcgccgaaac cttggagcag ttcgagtgga tcagcgcgag caaccgccgc
     1321 gtgagaaacg tcgagggtca cattcgggta gtcggcgtcg agcaatgacc aggacggcga
     1381 gtagaccccg agctcgagtc cgctcggccc gcgttgaggg aacatgttct cgaccgcaaa
     1441 gacgatgtcg gtctcttcgt gcatcgcgac gatgccggca gtgaaccctt tcgcgtagcc
     1501 gcgctgccac ctaaagggtg gatgcgtcac gacgaccttc gcgccgagcc gttcggccgc
     1561 gatctgcgag cgcatcaact tcgcccacgg ctcagtaccc caaacccgct gactgatcag
     1621 caggcagggc gaatgcactg acaccactgg catttggtga tagtcgacga gccttcgcaa
     1681 cgtgtcgaga tcctggctca cgggatcacc ggagaccatc aactcgacgc cgtcataacc
     1741 caaccgcgca gccacctcga acgccactgc ggttgattcg ggccacaccg acgaggtcga
     1801 caaggtcaca gccgggctcg ccccggctcc ctccagtgct ccgcccattc tccgagccta
     1861 cgacgccgcg ggccgacaca ccggttgacg tggatcggcg tccggcccag atctcttggg
     1921 gcctagtctt tgcgcatgcc acgcaagcca tcgaaatcat cgccgccgtc gcggccagct
     1981 gaatcgtctc gcgccgcgac tcggcccgcg ggcaagacga agacggccgc cgctgaaccc
     2041 gcgcacccca tcatccccac gcaagctggt gcgtcggcca gcgaacgcat accgctgccc
     2101 gaaaagggcc tcgacttcgc ccgcgagtgg gtcgagttcg tcgacccagc tgaccccgaa
     2161 cagatctttc gttgcgacct cacctggttg acgtcgagct ggggatgcat cttcggcaac
     2221 ggttgtcacg gcatcgtgcc gggccgggcg gctgacggct gctgcaccca tggcgccttc
     2281 tactcgggtc gcgccgacga gaagcgggtg cgtgagttcg ccgaacaact cacacccgag
     2341 acatggcagt tctacaagaa gggccagaag ggcttcaccg aactcgaaga cggcaagcgt
     2401 cgcacccgcc gagtcgatgg tgcgtgcatc ttcttgaacc gccctggctt cgagggtggc
     2461 gtcggctgct cgctgcacag cctcgcgctg acaatcggtc gccacccgct cgaaaccaag
     2521 ccggacgtct gctggcagct tcctatccgc cgcacgttcg actggatcga tcggcctgac
     2581 gagaccaagg tcttgatcgt ctcaatcggt gagtacgacc gcagaggctg ggggtcaggt
     2641 ggccacgacc tcaactggtg gtgcaccgca tcgccagaag cccacggcgc gcgcgaccgc
     2701 gtctacatct cctacggacc cgagctgacc gagctcatgg gcccgcaggc gtacgcgaca
     2761 ctggtcgagc actgcgagcg ccggctcgca tcgaagacgc cggtcgctcc gcaccccgcc
     2821 gaccctgtgt aggcggttca aggcgacgtc agaccgaagt aataaccaca ggtcgtccgg
     2881 cgaggacccg gattgtcatg atgcggcgct agcctgctgc tcatgcccaa gaccagcacg
     2941 cccggtcagg tcaagctgtc gtttcgctgc ggcgagtgcg ggtgggagtc gctgaaatgg
     3001 gtcggtcgct gcggtgaatg ccaggcctgg ggcaccgtcg aacagctagg cctgccgcgg
     3061 cttcgcagcg tcgccgccac atcggtcact gctgccgcac gccccatcgg tgatatcgat
     3121 gccgaagcag cacgcagccg accgaccggc gtcgacgaac tcgaccgggt gctcggcggc
     3181 ggtctcgtgc ctggcgtcgt ggtgttgctc gcaggcgaac cgggtgtcgg caagtcgacg
     3241 ctcgtactcg acgtcgcgtc gaaattcgcc ggtctcgaca cacccgactt cgccggcgcc
     3301 cgcacgctct acatcaccgg cgaagagtcg accgcgcagg tgcgtcttcg ggccgggcgc
     3361 atcaaggcgc tctcgccgca tctgtttctc gcggctgaaa ccgacatcgg ggccgtgctg
     3421 aagcacgtcg atgacgtcga tcctgcgctg ctcatcgtcg actccgttca aaccatggcg
     3481 atcgccgacg tcgatgggtc ggcgggtggc gtgacacaag tacgtcaggt ggcagccgc
//
LOCUS       DASWVF010000433         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_393825, whole genome shotgun sequence.
ACCESSION   DASWVF010000433 DASWVF010000000
VERSION     DASWVF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393825"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2002
                     /gene="secD"
                     /locus_tag="VGD55_07825"
     CDS             137..2002
                     /gene="secD"
                     /locus_tag="VGD55_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0870292.1"
                     /translation="MPPAKRSSSGRPLLLVLLLLAVLYGLMALGGKYSPALGLDLRGG
                     TTVTLEPKSTVGTKVTNGDLNTAKNILANRVNSLGVSSAEVKLEGKDVVISVPGKDTS
                     GVLKNIGETALLSIRQVYETSDTGLTGTDVQDTTSQTVTPSVSPSTSGAPSATPSAST
                     SATPSTSASPSASATSSPSAAPSSSPQALGVPGAAAGVQAQPAAFVRSAASAGPASTP
                     AGASAISAASPDAAASPAASAAPSASASTGATATPETTPSAAELAAYANLDCSLPANQ
                     QGGVHVDDPTKFLVTCGNPDGSTVYFKYLLHPTLILGKDVSTASAQLDTQTGTLWDVE
                     LTFKSGAASTWAKFTAAHVGTATAIVLDSNVFSAEVIQGAIVGPTQITGTFTHKTASN
                     LADVLKYGALPLAFQQQTAQTISATLGSNELKAGLLAGGIGLVLVLFYSFFYYRGLSF
                     VTVTSLALSAAIQYPIIVLLGKAISYTLTLAGIAGLIVAIGVTADSFVVFFERLRDEV
                     RDGNSLRTSVERGWVRARRTIISADVVSLIASVILYLLAIGDVRGFAFTLGLSTVVDL
                     VVVFLFTKPLLTLLAKTKFFSDGHRLSGLDAARLGVDRLRPATSTARRVTPARKV"
     gene            2004..>2593
                     /locus_tag="VGD55_07830"
     CDS             2004..>2593
                     /locus_tag="VGD55_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0870293.1"
                     /translation="MSRLGTIGHQLYTGQASYEFVGKWKRWVALSTVVMVIGLIGLAT
                     RGLSLSLEFKGGTQFTFPDKGYTVSQVSDVTSAAGVKSPVVQRLSNVGVGGANYQVES
                     KPLSQDEITKVTDAIVSHFNVTNQQVSLETVGSSWGSQITHKAVESLIIFLLAIIVYL
                     SLRYEWKMAAAAIVALIHDLIITVGIYALVGFDVSPS"
BASE COUNT          438 a          849 c          793 g          513 t
ORIGIN      
        1 gggttcgggg cccgttcctc aggcctgacc tggtgtcgct cggccctgcc ttcgtgccgg
       61 tccggtaact ccgctgcaac aatgtggtgt tcggcggtcc gcaagcagta tccgtaggtc
      121 tgacagggag attccggtgc cacctgccaa gaggtccagt tcagggcgtc ccctgttgct
      181 ggtcctgcta cttcttgccg tgctttatgg actgatggcg ctcggtggta agtactcacc
      241 ggcacttggt ctcgacctgc gcggcggcac gacggtcacc ctcgagccga agtcgacagt
      301 aggcacgaaa gtcaccaacg gcgacctcaa cacagccaag aacatcctgg cgaaccgggt
      361 caacagcctg ggcgtcagca gcgctgaggt gaagctcgag ggcaaggacg tcgtcatctc
      421 ggtgcccggc aaagacacct ctggcgtgct gaagaacatc ggcgagaccg cgttgctgag
      481 catccggcag gtatatgaga cgagcgacac cgggctgacg ggcaccgacg tccaggacac
      541 cacgtcgcag acggtgacgc cgtcagtctc gccgtcgact tcaggtgcgc cgtccgcgac
      601 accgtccgcc tcgacgtccg ccactccgtc gacttcagca agcccgtcgg cgtccgcgac
      661 gtcgtcaccg tcggctgcgc cgtcctccag cccgcaggcc ctcggcgtcc ccggcgctgc
      721 cgcgggtgtc caagcccagc ccgccgcgtt cgtgcgatcg gcagccagtg ctggtccagc
      781 ttccacgcct gctggcgcct cggcgatctc cgcggcatca ccggatgcgg cggcgtcgcc
      841 agccgcctcg gccgccccgt cggcgtccgc atcgactggc gcgacggcga cgccggagac
      901 cacgccgtcg gcggctgaac ttgcggcata cgcgaacctc gactgttcgt tgccggccaa
      961 tcaacagggg ggcgtgcatg tcgacgaccc gaccaagttt ttggttacct gcggcaatcc
     1021 cgacggttct acggtctact tcaagtacct gctgcacccc acattgatcc tgggcaagga
     1081 cgtctcgacg gcgtcggcgc agctcgacac gcaaaccggc accctgtggg atgtcgagct
     1141 caccttcaag tccggtgcgg ccagtacgtg ggccaagttc accgcggcac atgtcggcac
     1201 agcgacggcc atcgtgctcg actcgaacgt gttctccgcc gaagtcattc agggcgccat
     1261 cgtcggccct acgcaaatca ccggcacctt cacccacaag acggcgtcca acctcgccga
     1321 cgtcttgaag tacggcgcgt tgccgctcgc gttccagcag caaaccgcgc aaacgatctc
     1381 ggcgaccctc ggtagcaacg agttgaaggc cggcctgctc gctggtggca tcggtctcgt
     1441 actggtgttg ttctattcgt tcttctatta ccgcggtttg tcattcgtga cagtgacgag
     1501 cttggcgttg tccgcggcta tccagtaccc gatcatcgtc ttgctcggga aagccatcag
     1561 ctacacgttg acgctcgccg gcatcgccgg actgatcgtt gccatcggtg tgacggccga
     1621 ctcatttgtc gtgttcttcg aacgattacg cgacgaggtc cgcgacggca attcactgcg
     1681 gacgtcggtt gagcgaggct gggttcgcgc tcgacgaacg atcatttcgg ccgacgtcgt
     1741 ctcgcttatc gcgtcggtca ttctgtatct gctcgccatc ggcgatgttc gcggattcgc
     1801 cttcacgctc ggactttcga ccgtggtcga cttggtggtg gtcttcctgt tcaccaagcc
     1861 gctgctgacg ttgctggcaa agaccaagtt cttcagtgac gggcaccgac tgtcgggtct
     1921 tgatgcggcg cgtctcggcg tcgatcgact cagacccgcg acttcgactg cgcggcgcgt
     1981 gacgccggcc aggaaggtct gacatgagcc gcctcggcac gatcggccac cagctctaca
     2041 ccggccaagc gtcgtatgag ttcgtcggca agtggaagcg atgggtagcg ctctcgactg
     2101 tcgtgatggt gatcggcctg atcgggctgg ccacccgtgg gctgagcctg agccttgagt
     2161 tcaagggcgg tacccagttc accttccccg acaagggcta cacggtcagc caggtcagcg
     2221 acgttacttc cgcggcaggc gtcaagtcgc cagtcgtgca acgcctgtcg aacgtcggag
     2281 tcggcggcgc gaattaccag gtcgagagca agcccctcag ccaggacgaa atcaccaagg
     2341 ttaccgacgc gatcgtctcg catttcaatg tgacgaacca gcaggtgtcg cttgagacgg
     2401 tgggttccag ttggggatcg cagatcaccc ataaggcagt cgagagcctg atcatcttcc
     2461 tgcttgcgat catcgtgtac ctgtcgcttc gctacgaatg gaagatggct gcggcggcga
     2521 tcgtcgcgct gattcacgac ctcatcatca cggtcggcat ctatgcgctc gttggattcg
     2581 acgtctcgcc gtc
//
LOCUS       DASWVF010000434         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_266534, whole genome shotgun sequence.
ACCESSION   DASWVF010000434 DASWVF010000000
VERSION     DASWVF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266534"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VGD55_07835"
     CDS             complement(<1..669)
                     /locus_tag="VGD55_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0870294.1"
                     /translation="MHNGTPNSRLMMASWVRDAEQRGVHLLSYDRPGYGGSTAKPGRS
                     IADCTHDVRVIAQAFGIKRLGVWGVSGGGPHALACAALLPDLVTAVASLCSPAPYGGE
                     GLDFFAGMGQDNADDMQLFLDDPPAAREKCRADREDMLAATPDWIHQGLASLLSPTDA
                     NALTLEVATELLAAFQSGLAPGDEGWWEDNVAEMEPWGFDLADIQTPVQLWHGAQDRF
                     VPFQH"
     gene            complement(1215..2411)
                     /locus_tag="VGD55_07840"
     CDS             complement(1215..2411)
                     /locus_tag="VGD55_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870295.1"
                     /translation="MVKRALAWAGGLVLLALVVASVPLTRAAHQSSTASDVGVLVAFA
                     TFGGVGVVVAVRQPRNVMGWILIGIPVCLVLNTDMSAYAAIDYRVHPGRLPVGPLAVQ
                     LQPSWAPAVVLLGLAILLFPDATLPSIWWRRSVIAFLTLSAVWMVGAFGIATDTILLH
                     KIVVDASGNLTTINHPAGDWAWWGVVQDVFFPVLAVSSVAAIARQVATYRSSTGERRL
                     QFKWGMAGATMFVIGVVPLLTVSSSASATWRLISALAPIAVACLPLTIGVAILKYRLY
                     DIDRLISRTLSYALITGLLLGVYVGIVVLTTDVLSFSSPIAVAASTLAAAALFSPVRT
                     RVQRLVDRRFNRVRYDAVATVAAFSARLREAVDLDAVRADLLEVVGRAVEPAHAAIWI
                     NHGSVS"
     gene            complement(2605..>3100)
                     /locus_tag="VGD55_07845"
     CDS             complement(2605..>3100)
                     /locus_tag="VGD55_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0870296.1"
                     /translation="FAALSRRYSYRVADGWLDPLHRHDTLAHPKPLDLDAMNEASTLL
                     LGMHDFAAFCRRRVGATTQRTLLELTWSRDDEGVAIARVVADAFCHSMVRALVGALLE
                     VGDGRRDVHWPAAVLAGRKREDAAKVAPARGLTLEEVLYPDDAGLRARTEQTRRRRDQ
                     PDAY"
BASE COUNT          583 a         1041 c         1010 g          466 t
ORIGIN      
        1 gtgctggaac gggacgaatc gatcttgagc gccgtgccac aactgcacgg gagtctggat
       61 gtcggcaaga tcgaagcccc acggctccat ctcggcgacg ttgtcctccc accagccctc
      121 gtcaccggga gcgagtcccg actggaaggc cgccaggagt tcggtcgcca cctcaagggt
      181 cagtgcgttc gcatccgtcg gcgacagcag ggacgcgagc ccctggtgaa tccagtccgg
      241 tgtcgcagcg agcatgtcct cgcgatctgc acggcatttc tcccgtgcgg ccggcgggtc
      301 atcgagaaag agttgcatgt cgtcagcgtt gtcttgaccc atgccggcaa agaaatccag
      361 accctcaccg ccataagggg caggggagca cagcgacgct acggcggtca ctaggtccgg
      421 tagcagcgcc gcgcacgcca gtgcgtgggg accgccaccc gagacccccc agaccccgag
      481 ccgcttgatc ccaaaggcct gtgcgatgac cctgacatcg tgcgtgcagt cagcgatgct
      541 gcgtcccggc ttcgccgtgg aaccgccgta gcctggtcgg tcgtaactga gcagatggac
      601 tccgcgctgt tccgcatcgc gcacccacga cgccatcatg aggcgagagt tcggcgttcc
      661 gttatgcacc agaactggtc gacccgatgg gtcccccgcc gagtccacgc acagcgtccg
      721 accgtctggc gtcaccacat tcgacgtaga agttgtcacc gcgcgcaagc tacgcccatc
      781 cgaagaacct cgcacgcagt tacccagcgg gcgcgggcga actcaccgcc tgccccggtc
      841 cggggtaata cgcaagcatg cgcacggtac gaaccattga gtcgccacat cagcatgcgc
      901 cccagggctc gcgtgctcat cgtgacaacg cgcgaggcgc gttcatgtga acgcgaggtc
      961 caggtgccgc gttcacctga acgcgatgcc cgcgacgcgc ccgacgtcag atcacccagc
     1021 cgagacgcat gatcggtccg agacgtcgcc aggtccgcgg gcgagtcatc gcggcagtat
     1081 gggatgttcg gccgtccgcg gtctcggtag aacaatacgt tctccgatcc tgtggcccga
     1141 ccggtagcgc gctacgccgg gatctgaacg cagcgaacga acgaggcagt taccgcgtcg
     1201 gcgtagccgt gcggtcaact cacggagccg tggttaatcc agatcgcggc gtgtgctggt
     1261 tcaacggctc ggccaacgac ttccagcaaa tctgcgcgta ccgcgtcgag gtcgacggct
     1321 tctcgcagcc gcgccgagaa tgcggcgacg gtggcgacgg cgtcgtagcg gacgcggttg
     1381 aaccgtcggt cgaccaggcg ctgcacgcgg gtgcggaccg gactgaagag ggctgcggcg
     1441 gccagcgtgg acgcggcgac cgcgattggc gaggagaagg acagcacgtc ggttgtcagc
     1501 accacgatgc cgacatagac accgagcagg agcccggtga tcagtgcata ggacagggtg
     1561 cggctgatca accggtcgat gtcgtacagc cggtatttga ggatcgccac cccgatggtc
     1621 agcggcaaac aggccaccgc gatcggggcg agcgcactga tcagccgcca ggtcgccgat
     1681 gcgctgctgc ttaccgtcaa gagcggaacg acgccgatca cgaacatcgt ggcgccggcc
     1741 atgccccatt tgaattgcag gcgccgttca ccagtggaac tgcggtaagt cgcgacttgg
     1801 cgggcgatcg ccgcgaccga actgaccgcg agcaccggaa agaagacgtc ctggacgact
     1861 ccccaccacg cccagtcgcc ggccggatgg ttgatcgtcg tcaggttgcc gctggcgtcg
     1921 acgacgatct tgtggagcaa gatcgtgtcc gtcgcgatcc cgaacgcgcc gaccatccac
     1981 accgcggaca gggtgaggaa ggcgatcacc gatctgcgcc accagattga cggcagcgtc
     2041 gcgtctggga aaagcaggat cgccaacccg agcaatacga cggctggcgc ccacgacggc
     2101 tggagttgta cggcgagtgg tcccaccggc aaccggccgg ggtgcacgcg gtaatcgatc
     2161 gccgcgtacg ccgacatgtc cgtgttcagc accagacaca ccggaatgcc gatgagaatc
     2221 cagcccatca cgttgcgggg ctgacgcacc gcaaccacaa cgccgacgcc gccgaacgtc
     2281 gcgaacgcga ccaagacacc aacgtcgctg gctgtgctcg actgatgagc cgccctcgtc
     2341 agcggaacac tcgccacaac gagcgcaaga agcaccaggc cacctgccca ggcaagcgcg
     2401 cgtttcacca tcgacgcatc cctcgctgca attctcacca cgtgctcacc cgcgacgcta
     2461 ggggatcgac gttccgacat cgttacggat agcggaaacg ccgggctgga cgggaccggt
     2521 ggccaaacgt cctcaactcg gtagttgact gcgttcgcca accagctttg tgcgccagaa
     2581 acgtcacctg ttgccgtcgg gggactagta cgcgtcgggt tggtcgcgac ggcgcctggt
     2641 ctgttcggtt cgcgcgcgca gccccgcgtc atcgggatac aggacctcct caagcgtgag
     2701 cccacgggcc ggcgccacct ttgcggcgtc ctcgcgtttg cgtcctgcga gcaccgccgc
     2761 gggccagtga acgtcgcggc gcccgtcgcc gacctcgagc aacgcgccga cgagcgcgcg
     2821 caccatcgaa tggcagaacg cgtcggcaac gaccctcgcg atcgcgacgc cttcgtcatc
     2881 gcgactccag gtcagctcga gcaaggtgcg ctgcgtcgtc gcgccaacgc gacggcggca
     2941 gaaggccgcg aagtcgtgca tgccgagcag gagggtggac gcctcgttca tcgcgtcgag
     3001 gtcgagtggc ttcgggtggg ccagcgtgtc gtggcggtgc agcgggtcga gccagccgtc
     3061 ggcgacccgg tacgaatacc ggcggctcag cgccgcgaac
//
LOCUS       DASWVF010000435         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_470284, whole genome shotgun sequence.
ACCESSION   DASWVF010000435 DASWVF010000000
VERSION     DASWVF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470284"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VGD55_07850"
     CDS             <1..1266
                     /locus_tag="VGD55_07850"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0870297.1"
                     /translation="VDIVITEVGGTVGDIESLPFLEAARQVRHEVGRDNVFFLHVSLV
                     PYIGPSGELKTKPTQHSVAALRQVGIQPDAVVCRADRPIPQNVKRKISLMCDVDEEAV
                     IAAVDAPSIYDIPKVLHAEGLDAYVVRRLGLPFRDVDWTAWDHLLRRVHHPAHEVTIA
                     LVGKYIDLPDAYLSVTEALRAGGFANDARVNIRWVTSDDCSTPEGAAKALLGADGIVI
                     PGGFGVRGIEGKLGAIRYARENLLPTLGLCLGLQCLVIESARNVAGLPDANSAEFDAA
                     TPHPVIATMADQRDVIAGERDMGGTMRLGLYPADLLAGSIVREVYGEARVNERHRHRY
                     EVNNAYRAQLEAAGLVVSGTSPDGRLVEFVELPREVHPYFVATQAHPELRSRPARPHP
                     LFRGLVAAALARSAGDSPVDLRAEPVGTA"
     gene            1263..>1825
                     /locus_tag="VGD55_07855"
     CDS             1263..>1825
                     /locus_tag="VGD55_07855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0870298.1"
                     /translation="MTDVSNDPPLADTAADRVVVHSTQRFDGRVWDIRTDVVDLGDGQ
                     QVERDLVVHPGAVGIIAVDDDRQILLVRQYRHPVSALLWEPPAGLLDVAGEDPLAAAQ
                     RELFEEAGYRASRWSVLLDVFMSPGGSSESVRLFLAQDLTPVAEADRFIGEAEERAMI
                     TRWVPLAQARAGLFSGDLHSPLSVMGIL"
BASE COUNT          293 a          588 c          579 g          365 t
ORIGIN      
        1 gtcgacatcg tcatcaccga ggtcggcggc acggttggtg acatcgagtc cctcccgttc
       61 ctcgaggccg cacgacaagt gcgccacgaa gtcggtcgtg acaacgtctt cttcctgcat
      121 gtgtcgctgg tgccctacat cggcccgagc ggtgagctga agaccaagcc gacgcagcac
      181 tcggtcgccg cattgcgcca ggtcggtatc cagcccgacg ccgtagtgtg ccgggctgac
      241 cggccgatcc cgcagaacgt caagcgcaag atcagcctca tgtgcgacgt cgacgaagaa
      301 gccgtgatcg ccgcggtcga tgcaccaagc atctacgaca tccccaaagt gctgcacgca
      361 gagggcctgg acgcttacgt cgtgcgccga ctcggtctgc cctttcgtga cgtcgactgg
      421 accgcgtggg atcacctatt gcgccgcgtg caccaccctg cgcacgaggt gacgattgcc
      481 cttgtgggca agtacatcga cttgcccgac gcgtatctct cggtgaccga ggcacttcgc
      541 gcaggcggat ttgccaacga tgcgagggtg aacattcgtt gggtcacctc tgacgactgc
      601 tcgacacccg aaggcgccgc gaaggcgttg ctcggtgccg acggcatcgt cattccgggt
      661 ggattcgggg tgcgggggat tgagggcaag ctcggcgcca tccgatatgc ccgcgagaac
      721 ttgctgccca cactgggttt gtgtcttggc ttgcagtgcc tcgtcatcga gtcggctcga
      781 aacgtcgcgg gtttgcctga cgccaactcc gctgagttcg acgccgcaac gcctcatccg
      841 gtcattgcga cgatggccga ccaacgcgat gtcattgccg gtgagcgcga tatgggcggc
      901 acaatgcggc tcggcctata cccggccgat ctgctcgcgg gatcgatcgt tcgcgaggtc
      961 tacggcgagg cgcgcgttaa tgaacgtcac cgtcaccgct acgaagtgaa caacgcttac
     1021 cgtgcacagc tcgaagctgc cgggcttgtt gtctccggta cgtcgcccga tggccggctg
     1081 gtcgagttcg tcgagcttcc gcgcgaagtc cacccttatt tcgttgctac gcaggcacat
     1141 ccggagcttc gatcgcgacc agctcgtccg cacccgctgt ttcgtggcct ggtcgcagcc
     1201 gcccttgcgc gttccgccgg cgattcgccg gtcgacctgc gcgcagagcc cgtcggcact
     1261 gcatgaccga cgtgtctaac gacccaccac ttgccgacac tgccgccgat cgcgttgttg
     1321 tgcattccac acagcgtttt gatgggcggg tgtgggatat ccgcaccgac gttgtcgacc
     1381 ttggcgacgg gcagcaggtc gagcgagatc tcgtcgtgca tcctggggcg gtcggcataa
     1441 tcgcggtcga tgatgatcga cagatcctcc ttgtgcggca gtaccgtcac cccgtttctg
     1501 ccttgctgtg ggagccgccc gcggggctgc tcgacgtggc gggagaggac ccactcgccg
     1561 cagcccaacg tgaattgttc gaagaggccg gctatcgcgc ttcgcgctgg tcggtgttgc
     1621 ttgacgtctt catgtcaccg ggcggctcga gtgaatcggt acggttgttt ctcgcccagg
     1681 atctgacgcc ggtcgccgaa gccgaccgat ttatcggcga ggccgaggag cgcgcgatga
     1741 tcaccagatg ggtcccattg gcgcaggccc gtgccggcct gttctctggc gatctccaca
     1801 gcccgctgtc cgtcatgggg attct
//
LOCUS       DASWVF010000436         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_113437, whole genome shotgun sequence.
ACCESSION   DASWVF010000436 DASWVF010000000
VERSION     DASWVF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113437"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VGD55_07860"
     CDS             complement(<1..1100)
                     /locus_tag="VGD55_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870299.1"
                     /translation="MHQWPTNPVAAAGLIAIAVLICVALAGRSGSGFDATWALVWARD
                     LLHGHVAAAAPKGFTSTPHPATVLLAIAIRLIGAGHASAILWAAGIEIAAIALFGGLI
                     RAGQLVGAPLAGVCGALVLAVQPGLRDAIGRGTIDIVFAAVCVWAIVLMWRRPAWAIG
                     CASVAALCRPEGWWLLSVMLPLHWERSRPTIRLVVLGAMTITPIVWLVMGRAMFGDPL
                     AALRITVTNGNGLRTHHGMGALVHGVAAAPGIAAAVLLVSVVAAINNRRHIDALDLAT
                     VVGALMLGTLAFEVLVGAEFVDRYLIATIALMMPGGLRAVLALATRQSTPKLLPAAIC
                     WALVVIGVASTWSQYAVVRRNFAAQADDVASLA"
     gene            1462..>4156
                     /locus_tag="VGD55_07865"
     CDS             1462..>4156
                     /locus_tag="VGD55_07865"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEY0870300.1"
                     /translation="MSWAVYRGRHARRSIVGSATAVTLVASSLAFLQAVGGGRATAGP
                     PPGTALPNFARVSVASNGSQANGPSEQASVSSDGQSVAFTSFAGNLGPANGGIFLRNV
                     TSATTTPVYSGGASWPLASDGGAAVTYLADTNEYAGIQGGIYQWSAATNSTTTVLLQG
                     PNFADPQVSGRASASSDGSKVAYVVRDNTCSTADGPSFNLNISSTAWQFGSVYVWNRS
                     TGTSTLLESCAGDVAISPDGSTLAWVKDVGGALGVYTQNVASNAVNEVSLGHGGTPLD
                     ETEIEDPPTLSSNGRYVIFETDVLADGTPGSGSNYAGIRIVERDTTAAFTYSVPFQVT
                     AGAGHGSDPRPDQQVAPSVTADGHHIAYVYHPAYDAAVATLPSGQAMVANMVSGTLQR
                     ADVTARLRLPAPGYVLQQTAIAGAGRYVVFSSDATDLVPGDTNAPTFTAPRGGFDVFF
                     RDLTGTPPMIPAGEVRQGDAFCPVCQAIEARLDPINTATGAYETDAHDISLPGVGVPF
                     ALARSYSSDNTGVGDFGVGWQVNLTSHLAVLPSGDVQVTAGTGAVVVFHAQGDGTFVA
                     PPEVRATLTANADGSWQIETTDQLTSHYDASGRLTAQVDRNGQGLSYAYGVGGKLATV
                     TDAGGRVVQFTRGTSGPSAGLITAVSVPDGRAWAYTYSYGSDGVTPVLTSEVDPVAGA
                     TTYGYDANNLLATVTDPNGHVVVSVAYSGGTVSSLVEAGSSTPGSYQFNAATGTATTT
                     DPNGGVWTDTYLGNELTSETDPLGNTTNYLYDANGNRIAVTDALGNTTAFTYDANGNR
                     LSETDPLGNVTGYTYDTNNNMLSQTDPLGHVTTYTYDAHGNRLSQTDAAGGVHTWTYT
                     AGGQVASETDPLGHVTTYTYDASGDTTSTTVAAGTAIATTTI"
BASE COUNT          775 a         1417 c         1336 g          628 t
ORIGIN      
        1 gccaaggatg cgacgtcgtc cgcctgcgcc gcgaagttcc gccggacgac ggcgtactgc
       61 gaccaggtgc tggctacgcc gatgacgacc agggcccagc agatcgccgc gggaaggagc
      121 tttggggtgc tctgccgggt cgctagggca agcacagctc gaaggccgcc gggcatcatc
      181 agcgcgatcg tggcaatgag atagcggtcg acgaactctg cgcccaccag tacctcgaaa
      241 gccaacgtcc ctagcatcag cgcgccgacc acagttgcga gatcgagcgc gtcgatgtga
      301 cgtcgattgt tgatcgccgc aacaacggag acgagcagta cggcggctgc gatccccggc
      361 gcggcggcaa caccgtgcac gagggcgccc atgccgtggt gggtgcgaag gccgtttccg
      421 tttgtgacgg tgatacgcag tgcggctagc ggatcaccga acatcgcgcg acccatgaca
      481 agccacacga tgggagtgat ggtcatggcc ccgagcacaa cgagccgtat cgtcggacgg
      541 gatcgctccc agtgcagtgg cagcatcact gacagcaacc accaaccttc ggggcgacac
      601 agcgcggcga cacttgcgca cccgattgcc catgcgggtc ggcgccacat cagcacgatg
      661 gcccacacgc agacagcggc aaagacgatg tcgatcgtgc cgcgaccgat cgcgtcgcgc
      721 agccccggct ggactgccaa cacaagcgca ccacagactc ctgccagcgg agccccgacg
      781 agttgtccgg cgcgtatcaa tccgccgaac aaggcgatcg ccgcgatctc gatgcccgcc
      841 gcccacaaga ttgcggatgc gtggccggct ccgataagcc gaattgcgat agccagtagg
      901 acagtcgccg ggtgcggggt ggaagtgaaa cctttcggcg cggccgctgc gacgtgcccg
      961 tggagaaggt cgcgtgccca gaccaacgcc caggtggcgt caaaaccgga acctgagcga
     1021 ccggcgaggg ccacgcaaat caagacggct attgcaatca accccgcagc ggcgacggga
     1081 ttggtaggcc actggtgcac caggtcctcg gcagggcgaa cccgcagggc agctcgaggt
     1141 cgttcgcgtg cgattcgccg tcgccggtcg cgctcgcgga ctgctccggg catcttcatc
     1201 catcccgtat ggcctaccgg cgagcagggg catccgcgag cgagtttcac cgcatgtcgt
     1261 tcagctgtcc accagatgac gaacgcgctg accttagtac ggctgtaagc agtgctgccc
     1321 attggcccca aacggctttg aggcgcgcat ccgaccccgt tcacgacggt ctatcaaaga
     1381 cacgccaaga ccacctagac aggacggtcg atacacagca caatcggcgc gacaagttgg
     1441 cccgacgggg gagaactcgc catgagctgg gccgtctaca ggggtcgcca cgcccgccgg
     1501 agcatcgtcg gcagcgcgac agccgtcacg cttgtcgctt ccagtctcgc gttcctacag
     1561 gctgtcggcg gcggccgcgc gactgccggc ccccctcccg gtacggcgct gcccaacttc
     1621 gcacgtgtgt ccgtggcgtc gaacgggagc caggccaacg gtccgtccga gcaagcttca
     1681 gtgtcctccg acggccaatc cgtagcgttc acttcgttcg ccggcaatct cggaccagcc
     1741 aacggcggaa tcttcctgcg caacgtcacg tcggcgacca ccacaccggt ttacagcggt
     1801 ggggcgtcat ggccgttggc gtctgatggc ggtgccgccg tcacgtattt ggccgacacg
     1861 aacgaatacg caggcattca gggcggcata taccaatggt ctgccgcgac caactccaca
     1921 acgaccgtgc tcttacaggg gccaaacttc gcagatccgc aagtcagcgg gcgcgcttcg
     1981 gcgtcttcgg atggcagcaa ggtcgcgtac gtggtgcggg acaacacctg ctcgacggcg
     2041 gacgggccgt ccttcaacct gaacatttcg tccacggcct ggcagttcgg ctctgtctac
     2101 gtatggaacc gttcgaccgg cacctcaacg ctgctggaga gttgcgccgg tgacgtggcg
     2161 atctcacctg acggttcgac cctcgcgtgg gtcaaggacg tcgggggagc cctcggcgtc
     2221 tacacgcaga acgtcgcgtc gaacgcggtc aacgaggtgt cgctcggtca cggcggcacg
     2281 ccgctggacg agaccgagat cgaggatcca ccgaccctgt ccagcaacgg gcgctacgtc
     2341 atctttgaga ccgacgtcct tgccgatggc acgccggggt cgggcagtaa ctacgccggg
     2401 atcagaatcg tcgagcggga caccacagcc gcgttcacgt actcggtgcc gttccaggtg
     2461 actgccggcg ctggtcatgg ctcggatccg cgccccgacc aacaggtcgc gccgtccgtc
     2521 accgccgacg gccaccacat cgcctacgtc taccacccgg cgtacgacgc ggccgtcgcc
     2581 acgttgccgt ccggtcaagc gatggtcgcc aacatggtgt ccggcacgct ccagcgcgcc
     2641 gacgtgaccg cacggctgcg gttgcccgcc ccggggtacg tgttgcagca gacggcgatc
     2701 gccggcgccg gcaggtatgt ggtcttctcc agcgacgcaa ctgacctggt gccaggtgac
     2761 acgaacgcgc cgacgttcac cgcaccgcgc ggcgggtttg atgtgttctt ccgcgacctg
     2821 acgggcacgc cgccgatgat tcccgccggg gaggtccgcc agggcgacgc cttttgcccg
     2881 gtgtgtcagg ccatcgaggc ccggctggat ccgatcaaca cggcgaccgg cgcgtacgag
     2941 accgacgcgc acgacatctc gttgccgggc gtcggcgtgc cgttcgcgtt ggcccgctcg
     3001 tactcgtccg acaacaccgg cgtcggtgat ttcggggtgg gttggcaggt caacctgacg
     3061 tcgcatctgg cggtactgcc atctggcgat gtacaggtca ccgccggaac aggcgccgtg
     3121 gtggtgttcc acgcgcaagg cgacggaacg ttcgttgcgc cgccggaggt tcgcgcgacg
     3181 ctgacggcca atgcggatgg cagttggcag atcgagacca ccgatcagct cacgtcgcac
     3241 tacgacgcga gcgggcggct gaccgcccaa gtcgaccgca acgggcaagg actgagctat
     3301 gcctacggtg tgggcggcaa gctcgctacg gtcacagatg cgggcggccg cgttgtccag
     3361 ttcacccgag ggaccagcgg accgtccgcc gggctgatca ccgcggtgag cgtgcccgac
     3421 ggtcgcgcgt gggcctacac gtacagctac ggctctgatg gggtcacgcc ggtgttgacg
     3481 tcggaggtcg accccgttgc cggtgcgacc acctacgggt acgacgcgaa caacctgctg
     3541 gcgaccgtca ccgacccgaa cggacacgtg gtcgtgtccg ttgcgtacag cggcggcacg
     3601 gttagcagtc tcgtcgaggc cggcagttcg actccgggca gctaccagtt caacgccgct
     3661 accggtaccg ccacgacgac ggatccgaac ggcggagtgt ggaccgacac ctacctcggc
     3721 aatgagctga catcggagac tgacccgctc gggaacacga cgaactacct ttatgacgcg
     3781 aacggcaatc ggatcgccgt cactgacgcg ctggggaaca cgaccgcgtt cacctacgac
     3841 gcgaacggca accggctcag cgaaaccgac ccgctgggca acgtcaccgg ctacacctac
     3901 gacacgaaca acaacatgct gagccagacc gacccgctcg ggcatgtcac gacctacacc
     3961 tacgacgctc acggcaaccg tctctcccag accgacgcag cgggcggagt gcacacctgg
     4021 acgtacaccg cgggcggcca ggtggccagc gaaaccgatc cgctcggcca cgtcaccacg
     4081 tacacctacg acgcctccgg tgacacgacc tcaacgaccg tcgccgcggg caccgcgatc
     4141 gcaacgacca ccatcg
//
LOCUS       DASWVF010000437         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_419422, whole genome shotgun sequence.
ACCESSION   DASWVF010000437 DASWVF010000000
VERSION     DASWVF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419422"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGD55_07870"
     CDS             complement(<1..534)
                     /locus_tag="VGD55_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0870301.1"
                     /translation="MKARYLTAATLAAVIAVAGCSSSKSTKSATGSAPAGASTSAAAA
                     GASTPGAASGTLTVWLQTDAQTGWPKEVAAAQTAFNAKYPNVKVDVQYQTWADHLTKL
                     DAALAATPPDVVELGNTETTKYMADGALADISADKAKFDNAATWLKGLGDSVTYNGKT
                     FGVPYYAGARGVIYSTDA"
     gene            826..1581
                     /locus_tag="VGD55_07875"
     CDS             826..1581
                     /locus_tag="VGD55_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0870302.1"
                     /translation="MAARPQTLDEPSSSEPRVPKYYLLKQHLLDMTRTMPAGSPVPPE
                     RTLSIEFGTSRTTVRQALQELVVEGRLERIQGKGTFVAKPKVAQELRLTSYTEDMRAQ
                     GLEPTSTLLDIGYVLADEVLAEKLAIKRGARVLRIERLRLANGEPMAIETTHLSGQRF
                     PGLRRLLARSTSLYAVLSEAYGIKLLEAQETIETVPAPPREAHLLGTDVGLPLLLLSR
                     HSLDDAGLPIEYVRSLYRGDRYKFVAHLQRPTT"
     gene            complement(1756..2841)
                     /locus_tag="VGD55_07880"
     CDS             complement(1756..2841)
                     /locus_tag="VGD55_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0870303.1"
                     /translation="MTGTVTIEPFLRALVESGGSDLHCKVGSPPRVRINGSLRKLQTR
                     DLTAADTEAMVTEVMRKDLVELFARSNEADFAFSLSGVGRFRVNAFRSRGSAGLVFRR
                     VSVGAIPLEDLGLPSILGSLALEPRGLVLVTGPTGVGKSTTLAGMIDHINNNREVHIV
                     TVEDPIEVLHFDKLGMINQREVRVDTEDFSTALRAAMRQDPDVILVGEMRDQETVKAA
                     LAASETGHFVMSTLHTTDAQETVNRIIDFFPPHEQKQVRLSLASALRGIVCQRLVPRA
                     DGEGRCVTMEICINTGRIAEAIADPDKTSLINDLIAEGAYYGMQTFDQHLVALIRDGA
                     VTLEAAMGASTNPHDLTVELRRLGLVA"
     gene            complement(2947..3159)
                     /locus_tag="VGD55_07885"
     CDS             complement(2947..3159)
                     /locus_tag="VGD55_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEY0870304.1"
                     /translation="MAEEVRAEMVANVWKIVVAEGDHVEDGDTLAILESMKMEIPVIA
                     EASGTVSLKVAEGDVVQEGDLIAVIG"
     gene            complement(3189..>3369)
                     /locus_tag="VGD55_07890"
     CDS             complement(3189..>3369)
                     /locus_tag="VGD55_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870305.1"
                     /translation="DREAALELARAAVAAFEIEGLKTNLPFFAELLANEEFISGRYDT
                     GLIDRMRAPAPKPAP"
BASE COUNT          601 a         1077 c         1088 g          603 t
ORIGIN      
        1 ggcgtcggtg ctgtagatga caccgcgggc gcctgcgtag tacggaacgc cgaaggtctt
       61 gccgttatag gtcacggagt ccccgaggcc cttgagccag gtcgccgcgt tgtcgaactt
      121 cgccttgtcg gcgctgatgt cggcgagcgc gccgtcagcc atgtacttgg tcgtctctgt
      181 gttgccaagc tcgaccacgt cgggcggggt cgcggcgagc gcggcatcga gtttggtcag
      241 gtggtcggcc cacgtctggt actgaacgtc aaccttcacg ttcgggtact tggcgttgaa
      301 cgccgtttgc gcggccgcga cctccttcgg ccaaccggtc tgcgcgtcgg tctggagcca
      361 gaccgtcaag gtgccgctcg ccgcaccagg cgtggaagcg ccggcggctg ctgcggaggt
      421 ggaagcgccg gccggagccg aaccggtggc actcttcgtt gacttcgagc tgctgcaccc
      481 ggcaacagcg atcaccgcgg cgagtgttgc cgcggtcaga tagcgagcct tcacgtgcat
      541 cctcctcgaa atcacggtcg gcctcatgct gacgcggccg attggtgtag accattcggt
      601 aagttggcat agaccacttg cagtgtcaag gggttcgccg gaccccgcgg ccggtcgttt
      661 cgcaaccgtg atgtagtagg cgcagcaatg tcgcccatga gagcgctcgc actgcttcaa
      721 ctactagatt ccctagatgg tctagaccta tgagtgtgcg agtagctatg gtgggccacc
      781 gccgatccac ccggaccggc gtaccactgc gagcttgagg tcaacgtggc ggcaagaccc
      841 caaaccctcg acgagccgtc gagcagtgag ccgcgagttc cgaagtacta cctgctcaag
      901 cagcatctgc tcgacatgac gcgcacgatg ccggccggga gcccggtacc accggagcgg
      961 accctcagca tcgagttcgg gacgtcgcgg acaaccgttc gacaggcgct ccaggaactg
     1021 gtggtcgagg ggcgactgga gcggatccag ggcaagggca ccttcgtcgc caagcccaag
     1081 gtggcgcagg agttgcgcct gacgagctac accgaggaca tgcgcgcgca gggcctcgaa
     1141 ccgacgtcaa cgctgctcga catcggatac gtgctcgccg atgaagttct cgccgaaaag
     1201 ctcgccatca agcggggcgc ccgcgtcctt cggatcgaac ggctgcggct cgccaacggc
     1261 gaaccgatgg cgatcgagac cacccaccta tcgggccagc ggttccctgg gttgcgtcga
     1321 cttctcgccc gctcgacatc gctgtacgcg gtgctgtcgg aggcgtacgg catcaagctg
     1381 ctcgaggcgc aggagacgat cgagacggtg ccggctccgc cacgtgaggc gcacctgctc
     1441 ggcaccgatg tcggtctgcc gttgttgctg ctaagccgcc acagccttga cgacgcaggc
     1501 ttgccgatcg agtacgtccg ttcgctgtac cgaggtgacc gttacaagtt tgtcgcgcac
     1561 cttcagcgac caaccactta agcgacggcc acagggcaac ttcacatctg gtgtggtcgc
     1621 gaattcgcga ccacagcggc cgaatcgcaa ccacacgagg tgtgaagtgc cgcgcaaagg
     1681 gagccccgcc cagctgagct aggcgacgag gccctgtgtt cgcggttcgt ggcaagacaa
     1741 cccaagagcc ggtcgtcagg ccacaagacc caaccgacgg agttcgaccg tgaggtcgtg
     1801 cgggttcgtc gatgcaccca tcgcggcctc gagcgtgacc gcaccgtcgc ggatcagtgc
     1861 gacgaggtgc tggtcgaacg tctgcatgcc gtagtacgcg ccttcggcga tgaggtcgtt
     1921 gatgagtgag gtcttgtcag ggtcggcgat cgcttcggca atacggccgg tgttgatgca
     1981 gatctccatc gtcacgcagc gcccctcgcc gtccgcccga ggtaccaggc gctggcagac
     2041 gatgccacga agcgcactgg ccagcgacaa cctgacctgc ttctgctcgt gcggagggaa
     2101 gaagtcgatg atgcggttga ccgtttcctg cgcgtcggtg gtgtgcagcg tcgacatgac
     2161 gaagtggccg gtctctgacg ctgcgagcgc cgccttgacg gtctcctggt cgcgcatctc
     2221 gccgaccagg atcacgtccg ggtcctggcg catggcggcc cgcagagccg tggagaagtc
     2281 ctcggtgtcg actcgaacct cgcgctggtt gatcatgccg agcttgtcga agtgcaggac
     2341 ctcgatcggg tcttcgaccg tcacgatgtg aacttcgcgg ttgttgttga tgtggtcgat
     2401 catgccggcg agcgtcgtcg acttcccgac gccggtcggg ccggtcacca gcacgaggcc
     2461 acgaggctca agcgcgagcg accccaggat cgaggggagt ccgagatctt cgagcgggat
     2521 cgctccgacg ctgacgcggc ggaacaccag cccggccgaa ccacgggagc ggaaggcgtt
     2581 gacgcggaaa cggccgacgc ctgacaacga gaaggcgaag tcagcctcgt tgctcctggc
     2641 gaacagctcg acaaggtcct tgcgcatgac ttcggtgacc atcgcctcgg tgtcagcagc
     2701 ggtcagatca cgcgtctgaa gcttccggag cgacccgttg atgcgcacgc ggggagggga
     2761 accgaccttg cagtgcaggt cggacccacc agattcgacg agggcccgta gaaacggctc
     2821 gatcgtcacg gttccggtca cagtcattcc ttcgaccgct gaggaagctg ccttgaaccg
     2881 cccagagcct gaatccggaa agatcgccca cctgcactat ccgatggtgg cgatcaggtc
     2941 gccgcgttag ccgataacgg cgatgagatc gccttcttgc acgacatcgc cctctgcgac
     3001 cttcaggctg acagtgcccg acgcctcggc gatgaccgga atctccatct tcatcgactc
     3061 gagaatcgca agagtgtcgc cgtcctcgac gtgatcgccc tcggcgacca cgattttcca
     3121 gacattcgcg accatttccg cacgaacttc ctcggccacc aggtggctcc ttcctgttac
     3181 ggctctcgtt acggggccgg tttgggcgcg ggcgcgcgca tgcgatcgat caagccggtg
     3241 tcataccgac cactgatgaa ctcctcgttg gccagtaatt ctgcgaagaa cggcagattc
     3301 gtcttgagtc cctcgatctc gaacgcggcg accgcagccc gcgcgagctc aagcgcggcc
     3361 tcccggtcg
//
LOCUS       DASWVF010000438         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_368247, whole genome shotgun sequence.
ACCESSION   DASWVF010000438 DASWVF010000000
VERSION     DASWVF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368247"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VGD55_07895"
     CDS             complement(<1..1900)
                     /locus_tag="VGD55_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0870306.1"
                     /translation="MSIPEAVDPVEQSHREYDANLEVWPAQVAYLLRELDDVRAQLAD
                     SEETLRAIRHGEVDALVITAGAGSERVFTLSNADRPYRNFVENMSDGAATVSSEGIVL
                     YANQAFADLVALASHQVVGRPVLQFVTDASQARLADMLGPDSLGASVEASLSTTSGNT
                     VPVRISSSSALQVDGDEIICLTVMDLTRERLAEAALAHSAQHDALTNLPNRTLLTDRI
                     RHALERRRGEMSVLALLFCDVDGFKNINDAYGHQVGDDMLRLVAERLCTAVRPEDTVA
                     RIGGDEFVVLCESLDDYNDAATIAYRASAAVAAAITTGPPDLEVTVSIGVAVAAVGDE
                     ATADTLLRDADEAMYKAKRQGPNVVELFDEQLRDAASSSLRLLAELRRAEADHELRLH
                     YQPVLNLQSETVTGVEALIRWQHPTRGLVPPDDFIPFAERSGLVVGIGAWVMHEACEQ
                     AASWTIDMPPTAPQLSISVNVSGRQLAKRAGLVQAVYSALDESCLEPKSLVLEITESA
                     LMDDAETALRVLTELKSLGVRIAIDDFGTGYSSLVYLKRFPVDLLKIDKSFVAGLGRD
                     QEDAAIVRSVIDLAHALGIATVAEGVETPQHLAILQDLGCVYGQGYLWSPAVPAEQAL
                     GWAEHGSDL"
BASE COUNT          339 a          641 c          671 g          371 t
ORIGIN      
        1 gaaggtccga gccgtgctca gcccatccga gcgcttgttc ggccggcact gccggactcc
       61 acaggtagcc ctgtccatag acgcagccga gatcttgcag gatcgccagg tgctggggcg
      121 tctcgacacc ctcggcaacc gtcgcgatgc cgagtgcatg cgcgaggtcg atcacactgc
      181 gcacgatcgc ggcgtcctcc tggtcgcgtc caaggcctgc gacgaacgac ttgtcgatct
      241 tcaataggtc gaccggaaag cgcttcaggt acaccagtga cgagtaccct gtaccgaaat
      301 cgtcgatcgc gatccgcacc ccgagcgact tcaactccgt cagcacccgc aaggcggttt
      361 cggcgtcgtc catcaaggcg ctctcggtga tctcgagcac cagggacttc ggctcgaggc
      421 atgactcgtc gagcgctgaa tacactgctt gcacgagccc ggcccgtttg gcgagctggc
      481 gacccgagac gttgaccgag atgctcagct gtggcgcagt cggcggcatg tcgatggtcc
      541 agctcgccgc ctgttcgcag gcttcgtgca tgacccaggc tccgatacct acgacgagcc
      601 cactgcgttc agcgaacggg atgaagtcgt cgggaggcac taggccgcgc gtcgggtgct
      661 gccaacgtat cagcgcctcg acgccggtca cggtctcgct ttgcaggttg agcacgggct
      721 gatagtgcag gcgcagctcg tggtcggctt cggcgcggcg caactcggcg agcagccgca
      781 gactcgacga ggccgcatcg cgaagttgct cgtcgaagag ctcgacgacg ttgggacctt
      841 ggcgctttgc cttgtacatc gcttcgtctg cgtcgcgcag cagggtgtca gcggtggctt
      901 cgtctcccac cgccgcgacc gcgacgccga tactgaccgt cacctcaagg tcgggcggtc
      961 ctgtggtgat ggccgcggca accgcggcgc tcgcacgata ggcgatcgtg gcggcgtcgt
     1021 tgtagtcgtc gaggctctcg catagcacga cgaactcatc accaccgatc ctggcgacgg
     1081 tgtcttccgg gcggaccgcg gtgcacagcc gctcggcgac gaggcgcaac atgtcgtcac
     1141 ccacctggtg gccgtaggcg tcgttgatat ttttgaaccc gtcgacatcg caaaacagca
     1201 gggccagcac gctcatctcc ccgcgtctgc gctcaagggc gtgccggatg cgatcggtca
     1261 gcagtgtgcg gttcggcagg ttcgtcaggg cgtcgtgttg cgccgaatgc gcgagggctg
     1321 cttcggcgag ccgctcgcgg gtgaggtcca tgactgtgag acagatgatc tcgtcgccgt
     1381 cgacttgaag cgcagacgac gagctgatcc gaaccggcac cgtgttccca ctggtcgtac
     1441 tcagggaggc ctcgacggat gcgccaagac tgtcgggccc gagcatgtcg gcgagcctgg
     1501 cttggctcgc atcggtcacg aattgcagca ccgggcgtcc gacgacctga tggcttgcga
     1561 gtgcgactag gtcggcgaag gcctgattcg catacaacac gatgccctct gacgacacgg
     1621 tcgctgcgcc gtcggacatg ttctcgacga agttgcggta gggccgatct gcgttcgaca
     1681 gcgtgaacac gcgctccgac ccggccccgg ccgtaatcac gagcgcgtcg acctcgccgt
     1741 gcctgatcgc acgcaaggtt tcttctgaat cggcgagttg ggcgcgcaca tcgtcgagtt
     1801 ctcgtagcag gtaggcgacc tgagcaggcc aaacctcgag gtttgcgtcg tactcgcgat
     1861 ggctctgctc gacggggtcg acggcttcgg gaatgctcat cggtcttggg agacggagga
     1921 gtggtccttg atgccgagca gccgcaagac ccgcgccgtg tcggacaggt caccgacgag
     1981 ccggcgtttg ggtaggggcg attccctgat gagggtcggc gc
//
LOCUS       DASWVF010000439         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_266689, whole genome shotgun sequence.
ACCESSION   DASWVF010000439 DASWVF010000000
VERSION     DASWVF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266689"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGD55_07900"
     CDS             <1..558
                     /locus_tag="VGD55_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0870307.1"
                     /translation="SFAFVSVGTGIGMGLVLGGKLHRGAHGAAGEIGYLPFSEGHGAD
                     VEDARQRGSFEAASTAAAVVRAASRAGLADAASAQEVFSAASRGDAGAAAIVAEEALL
                     VAKAVCAVVTVVDPELIVLGGGIGQAPGFLAAVHAELVKMAPVLPDVKMSALGADAVV
                     EGCVAAGLDRAWELVIARIPAPTSE"
     gene            789..1061
                     /locus_tag="VGD55_07905"
     CDS             789..1061
                     /locus_tag="VGD55_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870308.1"
                     /translation="MEETTDEMKSEMTDETMYNVSADASYDDMMMAQDEGAQAEIATI
                     REAYAEKINAAIGANDDKLAIELSRQFRDEAFETLFGANAPRRNVA"
     gene            complement(1110..2948)
                     /locus_tag="VGD55_07910"
     CDS             complement(1110..2948)
                     /locus_tag="VGD55_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEY0870309.1"
                     /translation="MLVLVGIGLLAGLITSLSPCVLPVLPIVLAAGASPLVSEPVASA
                     ELVSPQLVNAGAKAALSRPRPTRPEPAPTRRWRVRSWRPYAVVAGLVLSFSASTLFGS
                     IVLSSLGLPLDLLRNVGIGVLVILGLGLIIRPLGELLERPFVRVTGRPVNPASNGLIV
                     GLGAGFLFVPCAGPVLATIAVVGATHRVGFSAVVLTLAFGIGVGVPLLALALAGDALS
                     RRAGALRRHAPRLRIAGGAVMILIAALIGFNLTDGLQRHVPGYTTALQNSIEGNKTAS
                     KQLQALTAAHPQKASSSSSSGSGTDSSSGCSEGGSTPQNCGLAPEFTGINAWLNTPGD
                     KPLTLAGLRGKVVLIDFWTYSCINCQRTLPHVEAWYRSYENAGLVVVGVHTPEFAFEH
                     VESNVSAQAHALGVKYPVAIDNDYATWNAYSNQYWPAEYLIDASGNIRHVTFGEGDYS
                     GTESVIRQLLTDANPTQVLPTATDVTDTTPTEQQTPETYLGYEYQLNYSGQVPAQDKT
                     QVYKFPATLQPDTFALSGTWDDGMQALTAGADAKLELSYQADIVYLVIGGTGTVSVAL
                     DGVTTSTIEISGVPKLYTLANTPTSDRRTLTLTASPGIEAYDFTFG"
     gene            3396..4856
                     /locus_tag="VGD55_07915"
     CDS             3396..4856
                     /locus_tag="VGD55_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY0870310.1"
                     /translation="MLTELATALHFAANTCTATAAPAACQPSWLDTGSNSWQLTAATF
                     VGLMSVPGLAVLYGGLVPKKWVVNTMFMAFTGFSAVLIVWVLWAYKMGFGAPVGGGTA
                     NTWSYTYGSNFFKNFFDNFVGHPETSLRGTSQISQATLTANGGTSLPLSQASAALFYF
                     QFVFAAITPLLFLGSVLGRIKVKVWLIFVPLWTTFVYAVNAMLIWGGGYWAHEGAVDY
                     SGGYVIHLAAGTSGFVAAWVIGPRLARDRAKFLPHSLPLVSIGAGIVWLGWNGFNGGD
                     PYFASADAATAVMNTNIATVSALLTWVLFDMFLSKSKKATFLGAVNGMIVGLVAITPG
                     AGFVNGFGALLEGIIASAIVWFAWTYLQPILIKRVDDAMGVFYTHGIAGLCGGILVGF
                     FADPSVVVYPLTTSGSPFAASGLIFGHPHSWKLIEEQLLAALTIIVWDAVVTFIILKV
                     ISMFTSLRMSDEELEIGDLAIHGEEAYPSDEGFARV"
BASE COUNT          873 a         1548 c         1631 g          907 t
ORIGIN      
        1 agcttcgcgt tcgtctcggt cggcaccggc atcggcatgg gcctggttct cgggggcaag
       61 ctgcaccgcg gggcgcatgg tgccgcgggc gaaatcgggt atttgccctt ttcggaaggg
      121 catggggcgg acgtcgagga cgcccgccag aggggtagct tcgaggctgc atcaactgct
      181 gccgcggtcg tccgggcggc cagccgggcc gggctggcag atgccgcctc ggcgcaagaa
      241 gtgttcagcg cggcctcccg gggcgatgcc ggcgccgcgg cgatcgtcgc cgaggaggcg
      301 ctgctggtgg ccaaggcggt gtgcgccgtc gtgaccgtgg tcgaccccga gctgatcgtt
      361 ttaggcggcg gaatcggcca ggcgcccggc ttcttggccg cggttcatgc cgaactcgtg
      421 aagatggcgc cggtgctccc cgacgtcaag atgagcgccc tcggtgccga cgcggtcgtc
      481 gaagggtgcg tcgcagcggg cctcgaccgc gcctgggaac tcgtcatcgc gcgtattccg
      541 gcaccaacgt cggaatgacg ctgaactcgg atcagtgacc ttcgtcacca ggaaataccc
      601 tcattcgagg ccgaattaca ggccatttga ggctccgcta atcatgaaca aatgagctcc
      661 cgcatttgcg gggaatgtca cacagtgttt tcccgttggt aacgcatgcg cggccctgtg
      721 ctgtcgatag aactggcaga gtgttgttcg tgtagaacgc gggcttacac aacgagggag
      781 ggcccacgat ggaagagacg acggacgaga tgaagagcga gatgacagac gaaacgatgt
      841 acaacgtgag cgcagacgcg tcttacgacg acatgatgat ggcgcaggac gaaggcgcgc
      901 aggcggagat cgcgaccatt cgcgaggcct acgctgaaaa gatcaatgcg gccatcggtg
      961 ccaacgacga caaacttgcg atcgaactgt cgcggcaatt ccgcgatgag gcgttcgaga
     1021 ctctatttgg agcgaatgct ccacgacgca acgtcgcgta ggagctagta cgccgatcca
     1081 ctattgattg ctccggccgc tgtaaacgct tatccgaacg tgaagtcgta ggcctcgatg
     1141 cccgggctcg cggtgagggt gagcgttcgg cggtcgctag ttggcgtgtt cgcaagcgtg
     1201 tacagcttcg gcacgccgct gatctcaatc gtcgaggtcg tcacgccgtc cagagcgacg
     1261 ctaaccgtgc cggtcccacc gatcacgagg tagacgatgt ccgcctgata gctcagctcg
     1321 agtttggcgt cagcgcccgc ggtcaacgcc tgcatcccgt cgtcccacgt acctgacaac
     1381 gcaaacgtgt cgggttggag cgtcgccggg aacttgtaca cctgagtctt gtcctgtgca
     1441 ggcacctgtc cggagtaatt gagctgatac tcatacccga gatacgtctc cggtgtctgc
     1501 tgctcggtgg gcgtggtgtc ggtgacgtcc gtcgcggtcg gcagaacctg cgtcggattc
     1561 gcgtcggtga gcagctgcct gatcacactt tcggtgccgc tgtagtcacc ctcgccgaac
     1621 gtgacgtgcc ggatgttgcc cgacgcgtcg atcaggtact cagccggcca gtactggttg
     1681 ctgtaggcgt tccaggtcgc atagtcgttg tcgatcgcaa ccgggtactt gacgccgagg
     1741 gcgtgggcct gcgccgagac gttgctctcg acatgctcga acgcgaactc gggtgtgtgc
     1801 accccgacca cgacaaggcc ggcattttca tagctgcggt accaggcttc gacgtgcggc
     1861 agcgtgcgtt ggcagttgat gcagctgtac gtccagaagt cgatgagcac gaccttgccg
     1921 cgcaggccgg cgagagtcag cggcttgtct ccaggggtgt tgagccacgc attgataccg
     1981 gtgaattccg gagccagtcc gcagttctgc ggcgtcgagc cgccttcgga gcaacccgag
     2041 ctcgagtccg tgccagagcc tgagctcgaa cttgagctgg ccttttgggg gtgagcggcg
     2101 gtgagcgctt gcaactgctt cgacgccgtc ttgttccctt caatcgagtt ctgcagcgcg
     2161 gtcgtgtagc ccggcacgtg tcgctgtagc ccgtcggtca ggttgaatcc gatcaaggcc
     2221 gcgatgagga tcatcactgc gccgccggcg atccgcagcc gcggggcatg gcgacgcaat
     2281 gcaccggccc gacgagacag tgcgtcgccg gcaagcgcca gcgccaagag cggcacgccg
     2341 acaccgatgc cgaacgcgag tgtcagcacg accgcgctga atccgacgcg atgcgttgcc
     2401 ccgacgacgg cgatcgtcgc cagaaccggg ccggcgcacg ggacgaacag gaacccggcg
     2461 ccaaggccga cgatcagccc gttgctggca gggttcaccg ggcggccggt cacccgtacg
     2521 aacggtcgtt cgagaagctc cccgagcggg cggatgatca atccaagacc gaggatgacg
     2581 agcaccccga tgcccacgtt gcgcagcagg tcgagcggca gccccagcga actcagcacg
     2641 atcgagccga ataacgtcga cgcgctgaag ctgaggacca gcccggcgac gacggcgtag
     2701 ggccgccacg acctaacccg ccagcgacgg gtgggtgccg gctctggccg ggtcggtctc
     2761 ggacggctga gtgctgcttt ggcacccgcg ttgacgagct gcggactgac cagctcagcg
     2821 ctggcgaccg gctcggagac gagcggcgat gccccggcgg caaggacgat cggcaacacc
     2881 ggcaggacgc acggcgacag gctcgtgatg aggccggcga gcagcccgat cccgaccaga
     2941 acgagcatcc tgcgctccct gttttctgct gacggacagc gacccgatgg caacctcgat
     3001 ggcaacccgc tgggaaccat cccagtcgca gtctcactca ccgccgggtg gctcgcgagg
     3061 tggtccgcat tacggtttgc ggacggttct tgggaagcgg gcggggtacc cgccgcagac
     3121 agcgcggacg tcgccgtgcc acccaatcca tcgccggtta cgtcgacgcg gcgcgcaggt
     3181 aaatagcgtg tgtcgcatcc ggagctagct gcgcgcccga gcttcccgat cacctcggct
     3241 ggaaccgact tcagcgcggc gcagccgcct tgggttcagt taacacacat gcaatggaca
     3301 cgtcgaatag gcgaaacacg gcgcatttac ggtctgcccg attcggtgcg cggccgcatg
     3361 gccgtctcgc ccaacccgtg gacaaggagg gcccgttgtt aactgagctc gcgacggcgc
     3421 tgcatttcgc agcgaacacg tgcactgcta ccgcagcacc ggccgcgtgt cagccgtcat
     3481 ggttagacac cggaagcaac tcttggcaac tcaccgcggc cacgttcgtc ggcctcatga
     3541 gtgtgcctgg tctggcggtt ctgtacggcg gcctcgtacc caagaagtgg gtcgtgaaca
     3601 ccatgttcat ggccttcacc ggcttttccg ccgtcctcat cgtgtgggtg ctgtgggcct
     3661 acaagatggg cttcggcgca cccgtcggcg gtggcaccgc gaacacgtgg agctacacct
     3721 acggcagcaa cttcttcaag aacttcttcg acaacttcgt cgggcacccg gaaacatcgc
     3781 tgcgcggtac gtcccagatc agccaagcga ccttgaccgc gaacggcggt acttcgttgc
     3841 cgctgagcca ggcgagtgcg gcgctgttct acttccagtt cgtgttcgcc gcgatcacgc
     3901 cgctcctgtt cctcggtagc gtgctcggcc gcatcaaggt caaggtgtgg ctgatcttcg
     3961 tcccgctgtg gacgacgttc gtctacgccg tgaacgccat gctgatctgg ggtggcggtt
     4021 actgggcaca cgagggcgcc gtcgactact ccggcggtta cgtcatccac ctcgcagctg
     4081 gtaccagtgg attcgtcgca gcctgggtga tcggcccgcg cctcgcacga gaccgcgcca
     4141 agttcttgcc acacagcctg ccgttggtct cgatcggcgc aggcatcgtg tggctcggct
     4201 ggaacgggtt caacggtggt gacccgtact tcgccagcgc tgacgcggca acggccgtga
     4261 tgaacaccaa catcgccacg gtctctgcgc tgttgacctg ggtgttgttc gacatgttcc
     4321 tgagcaagtc gaagaaggcc actttcctcg gcgccgtcaa cggcatgatc gtgggcttgg
     4381 tggctatcac cccgggagct gggttcgtca atggcttcgg cgccttgctc gaaggcatca
     4441 tcgcctcggc gatcgtctgg ttcgcgtgga cctacctcca gccgatcctc atcaagcggg
     4501 tcgacgacgc catgggtgtg ttctacacgc acggcatcgc cggtctgtgc ggaggcattc
     4561 tggtcggctt cttcgccgac ccaagcgttg tggtttaccc gctgaccacg agcggcagtc
     4621 cgtttgccgc gagcggcctg atcttcgggc acccgcactc gtggaagctg atcgaggagc
     4681 agctgctcgc cgcgctgacg atcatcgtgt gggatgcggt cgtgacgttc atcatcttga
     4741 aggtcatcag catgttcacg tcgctgcgaa tgtcggacga ggagctcgaa atcggcgact
     4801 tggccatcca cggcgaagag gcctacccgt cagacgaggg atttgcacga gtctgatcct
     4861 caagcgcgac cctacgtgcc aaccagcacg tagggccgcg cgttcagccg gtcatatact
     4921 caaacgagag cgggagcctg gcgatgggat cggcgatgg
//
LOCUS       DASWVF010000440         7631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_164296, whole genome shotgun sequence.
ACCESSION   DASWVF010000440 DASWVF010000000
VERSION     DASWVF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164296"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGD55_07920"
     CDS             complement(<1..471)
                     /locus_tag="VGD55_07920"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870311.1"
                     /translation="MIQSVDRAIRVLTALQGARRMTLSELATRLELPPSTVHGIVRTL
                     VGHGMVSQERGSSRYQLGPAVLLLGNVYLDTLELRSKAIPWAEDLARRTGAAVRTGVL
                     LLDDVVIIHHEPRPDGSRQMPEVGIVIPAHASALGKAMLVFDPDAADRVLSTTAL"
     gene            640..1425
                     /locus_tag="VGD55_07925"
     CDS             640..1425
                     /locus_tag="VGD55_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0870312.1"
                     /translation="MLDETSMAQRLTAEFIGTAFLVFIGVGSIPATLIVNGSAPFTFA
                     DLGMISFAFALIVIATVYAFGHIGGNHINPAVTLGLAVSGKFPWSQVPAYMGAQILGA
                     AAGAGAIVGTLGHKASTLGLGVAHYGPTIPVAQAFFAEFIGTFILVFTVFGVIHRKAA
                     AGFAGVAIGFVVFAVIMVVGPVTSSSINPARTIGPMIVQQFSGGAVLWHQLPVYLISE
                     LLGGALAAILYGLISTTPADRAGAGPGVDLADPADATYAHSSS"
     gene            1471..2472
                     /gene="dhaK"
                     /locus_tag="VGD55_07930"
     CDS             1471..2472
                     /gene="dhaK"
                     /locus_tag="VGD55_07930"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY0870313.1"
                     /translation="MKKLINDPANVVSEALLGIEAAHADLEVDHQNKIIYRGDGPHEG
                     KVGLISGGGSGHEPLHGGFVGLGMLDAACAGEVFTSPVPDQMLAATKLVDGGAGVLHI
                     VKNYTGDVMNFEMAAEMAAAETGTRVVSVVTDDDVAVQDSTWTAGRRGVGVTVLLEKI
                     AGAAAEQHRSLDHVAEIARTVNANGRSMGVALTSCTVPAAGKPTFELGETEMELGVGI
                     HGEPGRKRLPLASAREIAELIVEPILADLPFNRGDSVIAFVNGLGGTPLIELYVMYNE
                     VNKIVSGHGINIARSLVGSYITSLDMAGTSVTLLKVDPDLLALWDAPVKTPALRWGI"
     gene            2477..3127
                     /gene="dhaL"
                     /locus_tag="VGD55_07935"
     CDS             2477..3127
                     /gene="dhaL"
                     /locus_tag="VGD55_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY0870314.1"
                     /translation="MTEEVAAPVLDRWVRQFAALVAEQREYLTELDSAIGDADHGANM
                     DRGLKAAVAALDEAQPTTPGAVLSKVGITLVSTVGGASGPLYGTFFMRMGAAFGDTSA
                     ASPGQLAAALRAGLDGVVARGKAEPGDKTMYDALAPSVDALDAALASGQSLADALASA
                     RAAAEAGRDATTPMLARKGRASYLGERSVGHQDPGATSTALLVSALLTTVSAGSSV"
     gene            3133..5607
                     /gene="ptsP"
                     /locus_tag="VGD55_07940"
     CDS             3133..5607
                     /gene="ptsP"
                     /locus_tag="VGD55_07940"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0870315.1"
                     /translation="MSSESNVGIVVVCHSRTLAKAAVALASEMLHGRPVRFAVAAGLD
                     ETTFGTDAVRIKEAIEEVDGPAGVVVLMDLGSAVLSTELALDLLADPTARDRVVLSPA
                     AIVEGLVVAAVAAAGGASRLEVANEARGALLGKAAHLSGSDEIDEHLAPVVADVTAVF
                     TVTNRHGLHARPAARLVSELRSLDATVTLRNATTGTGPVPGSSLSRVATLGVLSGHEV
                     EVAATGPQAHEAVEHLVGLAERQFDEPDDDRNDAPADLAEAAAQGPRPASPGIAIGAA
                     RLLRSPLLADEAVEANGVVDVDAEWRRVVEAIASVRRDVERLRGLAAREMGAADAGIF
                     DAHLGLLGDTSLLADVKHGVAAGSTATAAWRSTIAAVEAQWAALPDPYLRARAADVRS
                     IGDDVLRAMTGAPRATITEPGILVAADLTPAEAAQLDRDLVIGIVLSHGSPSSHAAIL
                     ARSRGIPAIVGAGAGVLAVRDGTLLALDGASGELAVDPDAEALERFRALAAEQSRERA
                     RDLSRASESAITRDGEHVEVAANIGSVGDARSAFAAGADAAGLVRTEFLFLSRDRAPS
                     VNEQEAEYLALAAAIGGRRITLRTLDVGGDKPLPYVFAPLEQNPFLGRRGIRLALEQR
                     ELLTQQLEAICRTARQTPTSVMFPMVSTVQELLDSTSVLRAAAGAEGTPPDLRIGMMV
                     EVPAAALKIETFLPHLDFVSIGTNDLTQYTLAAERGNPAVAALSDALDPGVLQLIAQT
                     CNAARGRVPVAVCGEAASDATAVAILLGLGVRELSVAPAAVPRIKTLVRGLDLTQCVT
                     LAEKALTLRNANDVRQLVSAELGFSG"
     gene            5631..6554
                     /locus_tag="VGD55_07945"
     CDS             5631..6554
                     /locus_tag="VGD55_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0870316.1"
                     /translation="MPRVWVPYAEFVTDIESVGHDVRVDVYTGDDSPPESIDEVEFYV
                     APYSFTPYPLELTAKMGSLRVVQLLTAGFENALPFVRQDVTLCNARGVHDASTAELAV
                     TLMLSSLRGIPEFVLAQQRHEWLHVDRPSLADKTVLILGYGSIGAAIERRVLPFETRV
                     VRVASKARPDDEVHGVDEMPALLPLADIVVVIAPLTPTTRHLVDAKFLAAMRDGALLV
                     NVARGGIVDTDSLVAELLRGRLFAALDVTDPEPPPADHPLWSAPGLLLSPHVGGNTSA
                     FLPRAHALVRAQVARFVAGEPVENVVAGPPG"
     gene            6723..>7631
                     /locus_tag="VGD55_07950"
     CDS             6723..>7631
                     /locus_tag="VGD55_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870317.1"
                     /translation="MTRPESEFREFVGGFADPLARLAYLLTAGTDIEPIALTNDALAQ
                     VRRRWRDAETTGAPETQAVEALLAALPRRRKVASTPAAPDDARGLPSPEDERVAAHVS
                     NEAATAADVDVDALRDAIWTAWLTLAPRARVPIVLADASVASRRLAGIDVPETLGSAH
                     RQEAIADEGLRSLRARVVSDLPARGGTVSFPNQQLIGLLDDTLRQHAGSASVPIDPYP
                     RVLEHVGRQRRRAVSLVAVSLVAVAAAVTVAVRVSTPDKVTATDDAAAASRSGFLAAP
                     QVVPSAAGATASGLSAQGPFGRPPGRS"
BASE COUNT         1196 a         2563 c         2591 g         1281 t
ORIGIN      
        1 caacgcagtc gtcgagagca cccgatcagc agcgtccggg tcgaagacga gcatcgcctt
       61 gccgagcgcg ctggcgtgag cggggatcac gatgccgacc tcgggcatct ggcgactgcc
      121 atcgggccgc ggctcgtgat ggatgatcac gacgtcatcg agcagcagga cgccggtgcg
      181 caccgccgcg ccggtgcgcc gagccaggtc ttcggcccag gggatcgcct tcgaccgcag
      241 ctctagcgtg tcgaggtaga cgttgccgag cagcagcacc gccgggccga gctggtagcg
      301 gctcgagccg cgctcctggc tgaccatgcc gtgcccgacc agcgtgcgga cgatgccatg
      361 cacagtcgag ggcggcagct ccaggcgggt cgcgagctcg gagagcgtca tccggcgcgc
      421 gccttgcagc gccgtcagca cccggatcgc ccggtcgact gactggatca cgcgtcacct
      481 cctctcgggt tgtccagccg gccgctacca cgtacccacg cgtgaggcaa atctactgtc
      541 gaacgggtct tgccatgagt gaaccatgcg tgtaacttcc ctgtcacatt cgacaatgtc
      601 gaatgccatt cgacatatcc gacgcaagga aggggcacca tgctcgacga aacctctatg
      661 gcccagcggc tcacggccga gttcatcggg acggcgttcc tggtgttcat cggcgtcggc
      721 tcgatcccgg ccaccctgat cgtgaacggg agcgcgccat tcaccttcgc ggatctgggc
      781 atgatctcgt tcgcgttcgc cctgatcgtc atagcgacgg tctacgcgtt cggtcacatc
      841 ggcggtaacc acatcaaccc cgctgtgaca ctcggcctgg cagtttccgg caagttcccg
      901 tggagccagg tgcccgccta catgggcgcg cagatcctgg gcgcggcagc aggggccggc
      961 gcgatcgttg gaaccctcgg acacaaggcc agcacgcttg gtctcggcgt cgcccactac
     1021 gggcccacga tccccgtcgc tcaggcgttc ttcgccgagt tcatcggcac cttcatcttg
     1081 gtcttcacgg tcttcggcgt gatccaccgc aaggccgccg cgggcttcgc aggcgtcgca
     1141 atcggcttcg tcgttttcgc ggtcatcatg gtcgtggggc cggtcacgtc gtcgtcgatc
     1201 aaccctgctc gcactatcgg cccgatgatc gttcagcagt tctccggcgg cgccgtgttg
     1261 tggcaccaac tccccgtgta cctcatctcc gaactgctcg gcggcgcgct cgcagccatt
     1321 ctctacgggc tgatcagcac cacgccggct gaccgcgcgg gcgcgggtcc tggcgtcgac
     1381 ctggccgacc ccgcagacgc gacgtacgcc cacagctcca gctgaccgtt catccgcgat
     1441 cgtaagactc gacgaagtga agggacgctc atgaagaagc tcatcaacga tccggcgaac
     1501 gtggtgtcag aggcgctcct cggtattgag gcagctcacg ccgatcttga ggtcgaccac
     1561 cagaacaaga tcatctacag gggtgacggc ccacacgaag gcaaggtcgg gctgatctcc
     1621 ggcggcggct cgggccacga gccactgcac ggtgggttcg tcggtctcgg catgctcgac
     1681 gcagcctgcg cgggcgaggt cttcacctca cctgttcccg accagatgct cgcggccacc
     1741 aagctcgtcg acggcggcgc aggcgtgctg cacatcgtga agaactacac cggcgacgtc
     1801 atgaacttcg agatggcggc cgagatggct gcggctgaga cgggcactcg cgtcgtctcg
     1861 gtcgtgaccg acgacgacgt cgcggttcaa gacagtacgt ggacggccgg acgacgaggg
     1921 gtcggtgtca ccgtcttgct cgagaagatt gcgggtgcgg cggccgagca gcaccggtcg
     1981 ctcgaccacg tcgccgagat cgcacgcacc gtgaatgcca acggccgcag catgggcgtc
     2041 gcgttgacgt cgtgcacggt tcctgcggcc ggcaagccga ccttcgagct cggcgaaacc
     2101 gaaatggaac tcggtgtcgg cattcatggc gagccaggtc gcaagagact gccgctcgca
     2161 tcggcgcgcg agatcgccga actgatcgtc gagccgatcc tcgccgacct gccgttcaac
     2221 cggggcgaca gtgtgatcgc cttcgtgaac ggtctcgggg gcactccgct gatcgagctg
     2281 tacgtcatgt acaacgaggt caacaagatt gtctcggggc acggcatcaa catcgcgcgt
     2341 tcgttggtcg ggtcgtacat cacctcactc gacatggccg gcacgtcggt cacgttgctc
     2401 aaggtcgacc ccgatctgct cgcgctctgg gacgctccgg taaagacacc agccttgaga
     2461 tggggtatct gacgcaatga ctgaagaagt agccgccccg gtgctcgaca ggtgggtgcg
     2521 gcaattcgcc gcacttgtcg ccgagcagcg cgagtacctg accgaactcg actcagcgat
     2581 cggcgacgcc gatcacggag cgaatatgga ccgagggctc aaggctgcgg tggctgcttt
     2641 ggacgaagca cagccgacga cacccggggc tgtgctgagc aaggtcggca tcacgctggt
     2701 gtcgacggtc ggaggcgcga gcggcccgct gtacgggacg ttcttcatgc ggatgggagc
     2761 ggcgttcgga gatacgagcg ccgcctcacc cggccagttg gccgccgcgc ttcgtgccgg
     2821 cctcgacggc gtggtcgctc gtggcaaagc cgagccgggc gacaagacga tgtacgacgc
     2881 cctcgcgcct tccgtcgacg cgctcgacgc cgcactcgcg agcgggcaat cactggctga
     2941 tgcgctggcg tccgcgagag cggccgcaga ggctggtcgc gacgccacga cacccatgct
     3001 cgccaggaag ggacgtgcca gctatctcgg cgagcgcagc gtcggccatc aagaccccgg
     3061 cgcaaccagt accgcgctcc tggtctcggc gctgctcacg accgtaagcg ccggatcctc
     3121 ggtgtgatcg ctgtgtcgag cgaatcgaac gtcggcatcg tcgtcgtgtg ccacagccgc
     3181 acgctggcca aggccgcggt ggcgctggca tcggagatgc tgcacggtcg gccggtgcgt
     3241 ttcgcggttg ccgcaggtct cgacgagacc accttcggca ccgacgccgt ccggatcaaa
     3301 gaagccatcg aagaggtcga cgggcctgct ggtgtcgtcg tcctgatgga tctgggcagt
     3361 gcggtgttga gcaccgagct cgctctcgac ctgctcgccg acccgaccgc gcgcgaccgc
     3421 gtcgtgctct cacccgccgc catcgttgaa gggctcgtcg tcgcagcagt tgctgccgca
     3481 gggggcgcca gtcggctcga ggtcgcgaac gaagcgcgcg gtgcgctgct cggcaaggcg
     3541 gcgcacctgt ctggtagcga tgagatcgac gagcacctcg cgccggtggt cgccgacgtg
     3601 accgctgtgt tcaccgtgac caatcgccat ggcttgcatg cgcggcccgc ggcccgactg
     3661 gtgagtgaac ttcgaagcct cgacgcgacc gtgacgcttc gcaacgcgac gaccggtacc
     3721 ggtccggtgc cggggtcgag cctcagcagg gtggcgaccc ttggcgttct atcggggcac
     3781 gaggtcgagg tcgcggccac cggaccgcag gcgcacgagg ctgtcgagca cctggtcggg
     3841 ctcgccgaaa ggcaattcga cgagccggac gacgaccgca atgatgcgcc ggccgatctc
     3901 gccgaagccg ctgcgcaagg ccctcgtccg gcgtcacccg gcatcgccat cggcgccgcc
     3961 cgcttacttc ggtcgccgct gctggcagac gaggcagtcg aggccaacgg cgtcgtcgat
     4021 gtcgacgccg aatggcgccg ggtggtcgag gcgatcgcca gcgtgcggcg cgacgtcgag
     4081 cggctgcgcg ggctcgcggc gcgggagatg ggcgcggccg acgccggcat cttcgacgcc
     4141 cacctggggc tgctcggcga tacgtctctc ctggcggacg tcaagcacgg ggttgccgcc
     4201 ggatcgacgg cgacggctgc gtggcgctcg acgatcgcgg ccgtcgaggc gcaatgggcg
     4261 gcgctgcccg atccctacct acgagcgcgc gcggccgacg tgcgttcgat tggtgacgac
     4321 gtgctgcgtg cgatgaccgg cgcgccgcgg gcgacgatca ccgagccggg catcctggta
     4381 gcggccgatt tgacgccggc tgaggccgcg caactcgacc gcgacctcgt gatcgggatc
     4441 gtgctctcgc acggcagccc gagctcgcac gcggcgatcc tggcccgatc gcgcggcata
     4501 ccggcgatcg tcggcgctgg cgctggggtg ctcgcggtgc gcgacggcac gctgctcgct
     4561 ctcgacgggg cgtccggcga gcttgccgtc gaccccgatg ccgaagcgct cgaacggttc
     4621 cgcgcgctgg ccgccgagca gtccagagag cgcgcccgcg atctgtcgcg agcgagtgaa
     4681 tcggcgatca cgcgcgacgg cgaacacgtc gaagtcgccg ccaatatcgg ctcggtcggc
     4741 gatgcgaggt cggcgtttgc tgctggggcc gacgcggccg gtctggtgcg caccgagttc
     4801 ctcttcctca gccgcgaccg agccccttct gtcaacgagc aggaagccga atatctcgcg
     4861 ctcgcagcgg ccatcggtgg ccgccgtatc actttgcgca cgctcgacgt cggcggcgac
     4921 aagccgttgc cctacgtctt cgcaccgctc gagcaaaacc cctttctcgg acgacgcggc
     4981 atcaggctcg ccctcgagca gcgcgaactc ctgacgcaac aactcgaggc gatctgccgg
     5041 accgcccgtc agaccccgac cagtgtgatg ttcccgatgg tctcgaccgt gcaggagttg
     5101 ctcgacagca cgagtgtctt gcgggcggcc gcgggcgcag aagggacgcc gcccgacctc
     5161 cggatcggga tgatggtcga ggtgccggcc gctgccctga agatcgagac gttcctgccg
     5221 cacctcgact tcgtgagcat cggtaccaac gatctgaccc agtacaccct cgccgctgaa
     5281 cgcggaaacc cagccgtagc agcactttct gatgctctcg accctggcgt ccttcagttg
     5341 atcgcacaaa catgcaatgc cgcgcgtggg cgggtgccag tcgctgtctg cggtgaggca
     5401 gcctctgacg cgaccgccgt ggcgatcctg ctcgggctcg gtgtgcgcga actgagtgtc
     5461 gccccggctg cggtgcctcg tatcaagacg ctggtccgcg ggctcgacct cacgcaatgc
     5521 gtcacactcg cggaaaaggc cctcaccctg cgcaatgcga atgacgtgcg ccagctggtg
     5581 tcggccgaac tcggcttctc gggctgaacc tggcaggaca caatgggggg gtgccacgcg
     5641 tctgggttcc ctatgccgag ttcgtgaccg atatcgaatc ggtcggccac gacgtccgtg
     5701 ttgatgttta cacgggagac gacagcccac ccgagtcgat cgacgaggtc gagttctatg
     5761 tcgcgcctta ttcgttcacc ccgtacccac tcgagctcac cgcaaagatg ggttcgcttc
     5821 gagtcgtcca gctactgacg gcggggttcg agaacgcgct tccgttcgtg cggcaggacg
     5881 tcacgttgtg caacgcgcgc ggcgtgcacg acgcgagcac cgcggagctc gcggtcacct
     5941 tgatgctgtc gtcgttgcgc gggattccgg agttcgtcct tgcgcagcag cgacacgagt
     6001 ggttgcacgt cgaccgcccg tcgctggccg acaagacggt gctgatcctt ggctacggtt
     6061 cgatcggcgc cgcgatcgaa cgacgggtgt tgccgttcga gacgcgcgtc gtgcgggttg
     6121 caagcaaggc caggccggac gacgaggtgc acggcgtcga tgaaatgccg gcgctcttac
     6181 ccctggccga catcgtcgtc gtgatcgcac ctttgacgcc gacgacccgg cacctggtcg
     6241 atgcgaagtt ccttgctgca atgcgcgacg gcgcgttgct ggtcaatgtc gcgcgcggcg
     6301 ggatcgtcga caccgactca ttggtcgccg aattgctgcg cgggcggttg ttcgcagcgc
     6361 tcgatgtgac cgatcccgaa ccaccgccgg ctgatcatcc gctgtggtcg gcgcctggtc
     6421 tgctgctgtc accccacgtc ggcggcaata cgtctgcctt cttgccgcgc gcccacgcgt
     6481 tggtgcgtgc tcaggtggcc aggttcgtcg caggtgagcc ggtcgagaac gtcgtcgccg
     6541 ggccgcccgg ctgaggggtg aaccggactg gcggctgcgg cgtcttgggt ggagacgaac
     6601 cggtcgaggg cgcagaccga atgccctccg gaacgccctg agcgagagac aatcgcagat
     6661 ggggcaagta ggtcagggcc gccctttcgc gactcgtccc gattggttgg cagagggagc
     6721 agatgacgcg accggagagc gaattccgcg agttcgtcgg aggctttgcc gatccgcttg
     6781 cgcgactcgc ttacctgctc actgccggta ccgacatcga gccgatcgcg ctcaccaacg
     6841 atgcgctcgc ccaggtgcgg cgccgctggc gcgatgccga gacgaccggt gccccggaga
     6901 cgcaagccgt cgaagcgctg ctcgcagcct tgccccgccg gcgcaaggtc gcgtcaacgc
     6961 cggctgctcc cgacgacgcg cgcggtctcc cgtcgccgga agacgagcgc gtcgccgcgc
     7021 acgtaagcaa tgaggcggcc actgccgcgg acgtcgacgt cgacgcgttg cgtgacgcga
     7081 tctggacggc gtggctcacc ttggctccgc gggctcgggt accgatcgtt cttgccgatg
     7141 cgtcggtcgc ttcacggaga ttggccggga tcgacgtccc ggagaccttg ggctctgccc
     7201 accgccaaga ggcgatcgcc gacgaggggt tgcgaagcct acgggcgcgc gtggtgtcgg
     7261 acttgccggc tcggggcggc acggtgtcct ttcccaatca acaactcatt ggcttactcg
     7321 acgacacctt gcgccagcat gcgggttcgg cttcggtgcc gatcgatccg tatccgcggg
     7381 tgctcgagca cgtcgggcgc cagcgccggc gcgcggtgag tctcgtcgcg gtgagtctcg
     7441 tcgcggtcgc ggccgcagtg acggttgctg tacgggtttc cacccccgac aaggtcactg
     7501 caacggacga cgccgcagcc gcatcgcgca gcgggttttt ggcggcgccg caggttgtgc
     7561 cgtcggcggc cggagcgacg gcttctggcc tgtcggcgca gggaccgttc ggccgcccgc
     7621 ccggccgctc t
//
LOCUS       DASWVF010000441         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_266707, whole genome shotgun sequence.
ACCESSION   DASWVF010000441 DASWVF010000000
VERSION     DASWVF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266707"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGD55_07955"
     CDS             complement(<1..287)
                     /locus_tag="VGD55_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0870318.1"
                     /translation="MPADPDFPARASGNPDGLAAPALDPAIAARLKRDEHGLFPAVAQ
                     QHDTGEVLMVGWMDDEALHRTLTTGRATYWSRSRQEYWVKGQSSGHFQFVQS"
     gene            380..541
                     /locus_tag="VGD55_07960"
     CDS             380..541
                     /locus_tag="VGD55_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870319.1"
                     /translation="MTTAIPMPSIPTSRKEIRRRVRRQVMLRRLSRELSRTGDADSQI
                     VLRRPAPTT"
     gene            complement(554..1327)
                     /gene="hisF"
                     /locus_tag="VGD55_07965"
     CDS             complement(554..1327)
                     /gene="hisF"
                     /locus_tag="VGD55_07965"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0870320.1"
                     /translation="MTTTDALLAVRVIPCLDVDAGRVVKGVNFVDLRDAGDPVEMARV
                     YDAEGADEMVFLDITASSGDRETTYDVVRRTAEQVFIPLTVGGGVREVEDFNRLLRAG
                     ADKVGVNTAAVTRPDLLAEAADRFGAQCVVLSIDARRGATESGFEVTTHGGRYDTGID
                     AVAWAARAAELGAGEILLNSMDADGTLAGFDLELIAAVRALVSVPIIASGGAGSASDF
                     APAVLAGADAVLAASVFHFGRLRIGDVKAALQDAGQPVR"
     gene            complement(1324..1695)
                     /locus_tag="VGD55_07970"
     CDS             complement(1324..1695)
                     /locus_tag="VGD55_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0870321.1"
                     /translation="MIQRISSGSPFEAIVGYSRAVVAGPYVEIAGTTAPRAGDTSYAQ
                     AKGALEIIGSALAEAGLTFADVVRTRIFVTDISQWEEVGRAHGEVFGEIRPVSTMVEV
                     AALIDPAMLVEIEATAYRGPS"
     gene            complement(1780..>2121)
                     /locus_tag="VGD55_07975"
     CDS             complement(1780..>2121)
                     /locus_tag="VGD55_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0870322.1"
                     /translation="DVRGTTLSARGWTQDGGELFDVLARLDADGCARYVVTDVRRDGT
                     LTGPNLDLLRSVCAATDRPVVASGGVSSLSDLRALAELVPSGVEGAIVGKALYAGEFT
                     LEQALAEVAAN"
BASE COUNT          367 a          754 c          683 g          317 t
ORIGIN      
        1 gactgcacga actggaagtg accagagctc tgccccttga cccagtactc ctggcgcgag
       61 cgcgaccagt aggtcgcccg gccggtcgtc agcgtgcggt gcagcgcctc gtcgtccatc
      121 cagccgacca tcaacacctc gccggtgtcg tgctgctggg cgaccgcggg gaagagcccg
      181 tgctcgtcgc gcttgagccg ggctgcgatc gcagggtcga gagcgggcgc ggcaagaccg
      241 tcgggattac cggaggctcg cgcggggaag tctggatctg ctggcataca gccattgtgc
      301 cggggaccgg gcagcaccgt cgcgcgagat acgtgccgga acttctcatg gaggcgcatc
      361 agcttccgaa aggaacgccg tgaccaccgc gatcccgatg ccgtcgatcc cgacgagccg
      421 caaggagatc cgtcgccggg tccgtcgcca ggtgatgttg cgccggctgt cccgtgaact
      481 tagtcgcacc ggcgatgctg actcccagat cgtgctgcgc cgcccggctc cgacgacctg
      541 agccctccgc agctcagcgg acgggttggc cggcgtcctg caaggcggct ttcacgtcac
      601 cgatgcgcaa gcggccgaag tggaacacgc tcgccgcgag cacggcgtcg gcccccgcga
      661 gtacggcagg cgcgaagtcg gacgccgatc cggcgccgcc actggcaatg atcgggaccg
      721 acacgagtgc ccgaaccgcc gcgatcaact cgaggtcgaa gccggcaagg gttccgtcgg
      781 cgtccatcga attgagcagg atctcccccg cacccaactc agccgctcga gccgcccacg
      841 cgaccgcgtc gatgcccgtg tcgtaccggc cgccatgagt ggtgacctcg aaccccgact
      901 ccgtggcacc tcggcgcgcg tcgatcgaga gcaccacaca ctgcgcgccg aagcggtcgg
      961 cggcctcggc tagcaagtcc ggcctcgtca cggccgcggt gttcacgccc actttgtcag
     1021 caccggcgcg tagcaagcgg ttgaagtcct cgacttcgcg gactcccccg ccgacggtca
     1081 gtgggatgaa cacctgctcg gcggtgcgcc gcacaacgtc gtaggtcgtt tcgcggtcgc
     1141 cgctgctggc agtgatatcg aggaacacca tctcgtcggc gccctcggcg tcgtacaccc
     1201 gcgccatctc gacagggtcg ccggcatctc gcaggtcgac gaagttcacg cctttcacca
     1261 cacgcccggc atcgacgtcc aggcaaggga tgacgcggac cgcgagcagc gcgtcggttg
     1321 tcgtcacgag ggcccgcggt acgcagtggc ttctatctcg accagcatcg ccggatcgat
     1381 caacgccgcg acctcgacca tcgtggaaac aggccgaatc tcgccgaaca cctcaccgtg
     1441 ggcgcggccg acctcctccc actgactgat gtcagtgacg aagatgcgag tgcggacgac
     1501 gtcggcgaac gtcaacccgg cttcggcgag cgccgagccg atgatctcga gcgcaccttt
     1561 ggcttgcgcg taagacgtgt cgccagcccg cggcgcggtt gtaccggcga tctcgacata
     1621 cggccctgcg acgacagcgc ggctgtaccc gacgatcgcc tcgaacggcg atcccgacga
     1681 tattcgctga atcacgttcc caaccctagc catccgacga cagagcggac gtttactgcc
     1741 ctccaggggc ggtaaacgtc cactctgtgg ccgggcggat cagttcgcgg cgacctcagc
     1801 gagggcttgc tcgagggtga actcgcctgc gtacaaagcc tttccgacga tcgcaccctc
     1861 gactcccgac ggcacaagtt cggccagcgc ccgcagatcg gacagcgacg acacaccgcc
     1921 gctcgcgacg accggtcgat cggtagctgc gcacaccgac cggagcaggt cgagattcgg
     1981 ccccgtcagc gtgccatcgc ggcggacgtc ggtcacgacg tagcgcgcac acccatcggc
     2041 gtcgagacgg gcgagcacgt cgaagagctc gccaccgtct tgggtccagc cgcgtgcaga
     2101 caacgtggtg ccccgcacgt c
//
LOCUS       DASWVF010000442         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_342927, whole genome shotgun sequence.
ACCESSION   DASWVF010000442 DASWVF010000000
VERSION     DASWVF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342927"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGD55_07980"
     CDS             complement(<1..359)
                     /locus_tag="VGD55_07980"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEY0870323.1"
                     /translation="MNPAEAMSTCPGCGLVGAVSHGPCDPYGGSSPACWETFNAVTVK
                     DYGEYRYPEVHQLIVDAYMSQHAAFETAAGRRSVAVHLVGLYLALERGMTGPAVGRTL
                     GQVFPDKRDVASLAPKPG"
     gene            complement(430..>2657)
                     /locus_tag="VGD55_07985"
     CDS             complement(430..>2657)
                     /locus_tag="VGD55_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870324.1"
                     /translation="IAASAGFTPLAHQPPAFLTSCLLLTDGSLMCQEYFGNVWHRLTP
                     DAFGSYVNGTWDTPSIAPMPDGTDANAGCTPCAYRPLFFASQVLPDGKVVVIGGEDLL
                     GFGGWTNIGFLYDPVSNTWSSQLKEAFGGGNVGDATSNVLADGRMILASISSSNLEVF
                     NEATLSFTPLNPTGKADGNDEEGWNMLPNGNILTVDAGIPRQSEIYNPSTNTWGTPLS
                     TIVNLADTSDLGNSAEVGPATLRPDGILVAFSGNTLGQNAFFNTLTNTWSHPAGIDFP
                     LVPGQSNHYAVKDGPAATLPNGNVLVMASPVDTVFDHFNSPSHFWELGFTTNTLTQVA
                     DSPNAASFTSFEGHFTMLPSGEALFVGFNEGAVQDVDVYSNGGAPQNAWRPAITSSPA
                     TLSAGSLNNTISGTLFNGLSQGSAYGDDWQSYTNYPLVRITNTGTGHVFYARTHNHSR
                     MGVEAVGSTQTITTQFDVPSGVETGACTLNVVANGIASAPASCNVVGNTQTVACEAGL
                     SFLVDTGAHVQNGSGGPGEVNNNGTGTTVVSPTATTGNITSKAAVTIQHRSVVTGNVV
                     SASTVTKDSDATVTGTITANGTVTLPALPTLPAFPPPTLGSFTVNSGTLSRGPGSYVS
                     GQVSGGTLILSSGDYFFQSFTINAGGTVRATPTTRIFVQSALNFNASILASTGSPVQP
                     IQLGYAGTSNITLSARFDGTLIAPSAAVTFGTGSPLTFSGSFFAKTIEVAGSSTLACH
                     P"
BASE COUNT          452 a          838 c          872 g          495 t
ORIGIN      
        1 ccaggtttcg gagccagcga agccacgtcg cgcttgtcag gaaacacctg cccaagcgtc
       61 cgtccgaccg ccggccccgt catcccgcgt tcgagagcaa ggtacagccc aaccaagtgc
      121 acagcgacgg agcgacgccc cgcggccgtc tcgaacgccg cgtgctggct catgtacgca
      181 tcgacgatca gctggtggac ctcgggatac cggtactccc catagtcctt gaccgtcacc
      241 gcattgaacg tctcccagca cgcgggcgag gagccaccgt acgggtcgca aggaccatgg
      301 ctgaccgccc cgaccaaccc gcaccccgga cacgtcgaca tcgcctccgc gggattcata
      361 accccacgat tctgaccggc ggggcactaa cgggccacta cctgatcgtc gctcagtgat
      421 tggctctcgc tacgggtggc aggcgagcgt actgctgccg gccacctcga tcgtcttggc
      481 gaagaacgat ccggagaagg tcagcgggga acctgtcccg aacgtgaccg cggcgctcgg
      541 cgcgatcagc gtcccgtcga accgcgcgct cagcgtgatg ttcgacgtcc ccgcgtagcc
      601 gagctggatt ggttgcaccg ggctgccggt gctggccaag atcgatgcgt tgaaattcag
      661 ggcgctctgc acgaagatcc gcgtggtcgg tgtcgcccgc acggtgcccc ctgcgttgat
      721 ggtgaacgat tggaagaagt agtcaccaga tgacaggatc agggtgccgc cgctcacctg
      781 tccggacacg taagagccgg gtccccgcga cagcgtcccg ctgttgacgg tgaacgatcc
      841 cagagtcggc ggtgggaacg ccggaagcgt cggcagcgcc ggcagggtca ccgttccatt
      901 ggcggtgata gtgccggtca ccgtcgcgtc gctgtccttg gtcacggtgc tcgccgacac
      961 gacattgccc gtcacgaccg aacggtgctg gatcgtgacc gccgccttgc tcgtgatgtt
     1021 gcccgtcgtc gcggtggggc tgacgaccgt cgtgccggtc ccgttgttgt tcacctcgcc
     1081 cggaccgccg ctgccgttct ggacgtgagc gcctgtgtcg accaggaagc tcaggcccgc
     1141 ctcgcaggcg actgtctggg tgttgcccac gacgttgcag cttgccggag ccgacgcgat
     1201 gccgttggcc accacgttga gcgtgcaggc gccagtctcc acgccacttg gcacgtcgaa
     1261 ttgagtcgtg atggtctggg tgctccccac tgcttccacg cccatgcggc tatgattgtg
     1321 ggtgcgagcg tagaacacgt gtcccgttcc ggtgttggtg atgcggacca gcgggtagtt
     1381 cgtgtagctc tgccagtcgt cgccgtaggc gctgccctgc gacagaccgt tgaagagcgt
     1441 tcccgaaatg gtgttgttca ggctccccgc gctgagggta gcgggcgacg acgtgatggc
     1501 cggccgccag gcattctgag gcgcgccgcc gttcgagtag acgtccacgt cctggactgc
     1561 gccctcattg aacccgacaa acagggcttc accgctcggc agcatggtga agtgaccctc
     1621 gaaggaggtg aaggaggccg cgttcggcga gtccgcgacc tgggtcagcg tgttggtcgt
     1681 gaagccgagc tcccaaaagt ggctcggcga attgaagtgg tcgaaaaccg tatccacggg
     1741 tgaggccatg accagcacgt tgccattcgg taatgtggcg gccggtccgt ccttgacggc
     1801 gtagtggttg ctctgaccgg ggaccagcgg aaagtcgatg ccggccggat gggaccaggt
     1861 gttggtgagc gtgttgaaga atgcattctg acccagcgta ttgccactga acgcgaccaa
     1921 gatgccgtcc ggcctcaacg ttgcgggtcc cacctccgcc gagttgccca ggtcgctggt
     1981 gtcagccagg ttgacgatcg tcgagagtgg agtcccccag gtattggtcg acgggttata
     2041 aatctccgac tggcggggga tgcccgcgtc gacggtcaaa atgtttccgt tggggagcat
     2101 gttccatcct tcctcgtcgt tgccgtccgc cttgcccgtc gggttcagcg gggtgaacga
     2161 tagcgtggcc tcgttgaata cctcgaggtt cgacgagcta atgcttgcca gtatcattct
     2221 gccgtccgcc agcacgttcg acgtggcgtc acccacgttg ccgcccccga acgcctcctt
     2281 cagctgcgac gaccaggtgt tgctcacggg atcgtacaag aacccgatgt tggtccagcc
     2341 gccgaatccc aggaggtcct cgccgccgat gacgaccacc ttgccgtcgg ggagcacctg
     2401 tgatgcgaag aacagcggtc gataggcgca gggcgtgcat ccagcgtttg cgtctgtccc
     2461 gtccggcatg ggcgcgatgg acggcgtgtc ccacgttccg ttgacgtagc tcccgaatgc
     2521 gtcgggagtc aggcggtgcc aaacattgcc gaagtattcc tggcacatca gcgaaccgtc
     2581 ggtgagcagc aagcaggaag tcaggaaagc cggcggctga tgtgcaagcg gtgtgaagcc
     2641 cgcgcttgcg gcgatcg
//
LOCUS       DASWVF010000443         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_139393, whole genome shotgun sequence.
ACCESSION   DASWVF010000443 DASWVF010000000
VERSION     DASWVF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139393"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGD55_07990"
     CDS             complement(<1..881)
                     /locus_tag="VGD55_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEY0870325.1"
                     /translation="MHARDLRTATGESGAVAIIVALLAVTLFGFAALVVDIGNADSVH
                     AQGQSTVDAAALAGVRTLASGGTEDDVSDIVKHYVAANMGITNWSGCTDPTPLPVLSD
                     PNTVGSCVSTLTSGTPGVTSYQVRVKLPPRHVPSTFGGLFGVSSITISPVAQALSGQP
                     LPPECGPCDPAIDETTQQPMPQPPPTGLPAAIRAMLPNPLDGSVPPAGPVGPTGCPST
                     PGLFTTNVAIVSNSICDLNPGLYVFYNSNLDVEVNGSIASHLAPDNTGVTLVFYGTGS
                     ITVEGHIGILDSNGQRSP"
     gene            complement(863..1501)
                     /locus_tag="VGD55_07995"
     CDS             complement(863..1501)
                     /locus_tag="VGD55_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0870326.1"
                     /translation="MSTLLAVIGGLGRYPGLMRLSVRRSRAGWSPRRGRSPLEEGAAA
                     VEFALVLPVLILILFGIIDYGLYFGDALDTRSGVASATRQAVVDNFDATCLPGSGSAP
                     LDPDVGHLVCMVESRTAPLAGKTYVDVILPTDPANSGSSATGWFAGGSVVVCEATAVT
                     GVTGYVPLPTRGVIRTRLVSQIEQHTDTTTLNESGGFDFTPPGGWSWCTPAT"
     gene            complement(1625..>2253)
                     /locus_tag="VGD55_08000"
     CDS             complement(1625..>2253)
                     /locus_tag="VGD55_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0870327.1"
                     /translation="ANCHAWAETRFGAARGMTTVVAVILGTGIGGGIVLDGHLYRGGF
                     GLAGEPGHVRVVPDGRLCGCGNRGCWEQYASGNALVREARDMADSSPSSLPYLMAAVA
                     GDIDAITGPLVTEAAHAGDEGARRCFETIGRWVGQGLADLATILDPACFVVGGGPSDA
                     GAVLIDPVRESFAAALSGGAYRPHAQILAAELGSAAGLVGAADLARIR"
BASE COUNT          406 a          752 c          758 g          337 t
ORIGIN      
        1 ggtgaccgtt ggccgttgct atcaaggatg ccgatgtggc cctcaacagt tatggatcca
       61 gtgccataga agaccaacgt gacgcccgtg ttgtcgggcg ccagatgact ggcaatgctc
      121 ccgttgacct cgacgtcaag gttgctgttg tagaacacgt acaaaccagg gttgagatca
      181 cagatggagt tgctgacgat ggcgacgttc gttgtgaata gtccaggagt gctcgggcaa
      241 cccgttgggc cgacgggtcc cgcgggtggg accgaaccgt cgagcgggtt cggcagcatt
      301 gcgcggattg cggcgggcag tccggtcggc ggcggctgtg gcatcggctg ctgggtggtc
      361 tcatcgattg ccgggtcgca cggcccgcat tcgggcggca acggctgtcc cgacagtgcc
      421 tgcgcaaccg gcgaaattgt gatcgagctc acgccgaaca atccgccaaa cgttgacggc
      481 acatgcctgg gcggcaactt cactctgacc tggtacgacg tgacacccgg cgtccccgac
      541 gtcagcgtcg agacacaact gccgaccgta ttcgggtcag acagcacggg gagcggggtc
      601 ggatcggtgc accccgacca attcgtgatg cccatgttgg cggcgacgta gtgcttgacg
      661 atgtccgaga catcgtcttc agtgccgccg ctcgcgagcg tccgaacgcc cgccagcgcc
      721 gcggcgtcca ctgtgctctg tccttgcgcg tgcacgctgt cggcgttgcc gatgtcgacg
      781 acgagtgcgg cgaagccgaa tagcgtcacc gcgagtagcg cgacgatgat cgcgaccgct
      841 ccgctctcac cggtggccgt tctcaagtcg cgggcgtgca ccaggaccat ccgccaggcg
      901 gagtgaagtc aaagccgcct gattcgttga gtgtggtcgt gtcggtgtgc tgctcgatct
      961 gcgagaccaa cctcgtgcga atcacgccgc gggtgggcag cggcacgtag ccggtcacgc
     1021 cggtcaccgc ggttgcctca cacacgacga ccgaaccgcc ggcgaaccac ccggtggcac
     1081 tcgacccgga attggccggg tcggtcggca agatgacgtc gacataggtc ttgccggcta
     1141 ggggtgcggt gcgcgactcg accatgcaca caagatgacc gacgtccggg tcaagaggag
     1201 ctgagccact gccgggcaga cacgtcgcgt cgaagttgtc gacaaccgcc tgcctcgtgg
     1261 cgctggcgac gcccgaccgg gtatcaaggg catcgccgaa gtagaggccg tagtcgatga
     1321 tcccgaacag gatcaggatc agcaccggca acacgagcgc gaactcgaca gcggcagcac
     1381 cctcttcgag cggcgaacgc ccacggcgcg gcgaccagcc cgcgcgcgat cgtcgaacgc
     1441 tcagccgcat caggccaggg tagcgaccga gcccgccgat gacggccaac aacgtggaca
     1501 ctccgcgtcg ccagagcacg aggtgccgac aacgtggagt ctggtggtag ctagaggtac
     1561 cgcgaaactc cacgatcatc ggaaccgcga acagcagcga gccggacggc gaggggctag
     1621 cgcctcagcg gatgcgagcc aggtcggccg cgccgacaag acccgcggcg ctaccgagtt
     1681 cggctgccag gatttgtgcg tgcgggcggt aggcaccgcc gctgagcgcc gccgcaaacg
     1741 actcgcgcac cggatcgatg aggaccgcgc cagcgtcgga cggaccgccg cccaccacga
     1801 agcaggccgg atcgaggatc gtcgccaggt cggcaaggcc ctgcccgacc caccgaccga
     1861 tcgtctcaaa gcatcgccgc gcgccctcgt cgcccgcgtg cgccgcttcg gtcacgagcg
     1921 ggccggtgat cgcgtcgatg tcgccagcca ctgcggccat caggtacggc aagctggacg
     1981 gcgaggagtc ggccatgtcc cgagcttccc gaaccaatgc gttgccgctg gcgtactgct
     2041 cccagcaacc gcggttgccg catccgcaca ggcgcccgtc gggcacgacg cgcacgtgac
     2101 caggctcccc cgccaggccg aatccgccgc gatagaggtg gccgtcgagc acgatgccgc
     2161 ccccgatccc ggtgccgagg atcaccgcga cgacggtcgt catcccccgc gctgcaccga
     2221 atcgggtctc ggcccacgcg tggcagttgg cgt
//
LOCUS       DASWVF010000444         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_470512, whole genome shotgun sequence.
ACCESSION   DASWVF010000444 DASWVF010000000
VERSION     DASWVF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470512"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1237
                     /gene="aroC"
                     /locus_tag="VGD55_08005"
     CDS             59..1237
                     /gene="aroC"
                     /locus_tag="VGD55_08005"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041834988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0870328.1"
                     /translation="MLRWLTAGESHGPALVAILEGLPAGVHTSTHEVADALARRRAGY
                     GRGARMSFERDEIDFLGGIRHGVSMGGPIAIRVGNSEWPKWEKVMSPDPVDEDELADL
                     ARNAPLTRPRPGHADLVGMQKYALDDVRPILERASARETAARVALGDIAASFLRQALD
                     VEVLSHVVSIGQVSAPAGLVPTASDRDGIDEDLVRCFHPATSAAMVAEIDSARTEGDT
                     LGGVVEVVAYGLPPGLGSHVHWDRRLDSRLAGALMGIQAIKGVEVGDGFESARRRGSF
                     AHDEIENGPGGVRRRTNRAGGTEGGMSTGEVLRVRAAMKPISTVPRALDTIDVATGEA
                     AKAINQRSDVCAVPAAGVVAEAMVALVLADCVLEKFGGDSLSEVRRNVRGYLDTLVVH
                     "
     gene            1241..1741
                     /locus_tag="VGD55_08010"
     CDS             1241..1741
                     /locus_tag="VGD55_08010"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0870329.1"
                     /translation="MAPLVVLVGPPGAGKSTVGRLLASRFGTTFRDTDLDVEKVAGLK
                     VADIFFEHGEEHFRKLESVAVEHALAEHDGVLVLGGGAILDPHTRELLHGHRVVYIDV
                     DLAEAAKRVGFNRDRPLLLSNPRAQLHALMEARRPLYEEVATATVVSTGKAATAVTAE
                     IAQVIR"
BASE COUNT          288 a          541 c          613 g          316 t
ORIGIN      
        1 gggccgatgc aattcgagca cccgtcaagc ccggctcgtt catgggagac tcactcctat
       61 gctgcggtgg ctgaccgctg gcgagtcgca tggtcctgcg ctcgtcgcga ttctcgaggg
      121 cctgcctgct ggcgtgcaca cctccaccca tgaggtagcc gacgcgctgg cccgccgacg
      181 ggctgggtac ggccgaggcg cccggatgtc ctttgaacgt gacgagatcg acttcctcgg
      241 cggcatcagg cacggcgttt cgatgggcgg cccgatcgcg atccgggtcg gcaacagcga
      301 atggccgaag tgggaaaagg tcatgagccc cgatcccgtc gacgaggatg agctggccga
      361 cctcgctcgc aacgccccgc tcacgcgtcc tcgccctggg cacgccgact tggtcgggat
      421 gcagaagtac gccctggacg acgtccggcc gattctggaa agggcgagtg cccgcgaaac
      481 cgcggcccgg gtggcgctcg gcgatatcgc cgccagcttc ttgcgacagg ccttggacgt
      541 cgaggtgttg agccacgttg tctcgatcgg tcaggtgagc gccccggcag ggctcgtgcc
      601 gaccgcgagc gatcgtgacg gcatcgatga ggacctcgtt cgctgcttcc atccggctac
      661 cagtgcggcc atggtcgccg agatcgacag cgcacgcacc gagggagaca ccctcggcgg
      721 tgttgttgag gtcgtggcct atgggctgcc gccgggcctt ggcagtcacg tccactggga
      781 ccgccggctc gattcgcggc tggcgggtgc attgatgggc atccaggcga tcaagggtgt
      841 cgaggtcggt gacggattcg agtctgcgcg tcggcgcgga tcgttcgccc acgacgagat
      901 cgagaacggc cccggcggag tgcgccggcg gaccaatcgc gcgggtggca ccgaaggcgg
      961 gatgtcgacg ggtgaggtgt tgcgggtgcg cgcggcgatg aagcccatct cgacggttcc
     1021 tcgagccttg gacaccatcg acgtcgcgac cggtgaagcg gcgaaggcga ttaatcagcg
     1081 aagtgatgtg tgtgccgtgc ctgctgcagg tgtcgtcgct gaggcgatgg tggcgctggt
     1141 gctcgccgat tgcgtgctgg agaagttcgg tggtgactca ctgagtgagg tgcgccgcaa
     1201 tgtgcgtggt tacctcgaca ccttggtcgt gcactgaccg atggcgcctc tggttgtcct
     1261 cgtgggtccg cccggagcag gcaagagcac ggttggtcga ctgctcgcga gccgcttcgg
     1321 tacgacgttt cgcgacaccg atctcgacgt cgagaaggtc gcgggtctga aggttgcgga
     1381 catcttcttc gaacatggag aagagcattt ccggaaactt gaaagcgttg ccgtcgagca
     1441 cgcattggca gaacacgatg gggtgctcgt tcttggcggt ggggcgatcc tcgacccgca
     1501 tactcgcgaa ctcctgcatg ggcaccgagt tgtctacatc gacgtcgacc tcgccgaagc
     1561 agccaagcgg gtggggttca accgcgatcg cccgttgcta ctcagcaatc cgcgcgcgca
     1621 actgcatgca ctcatggaag ccaggcgtcc gctctacgaa gaggtcgcga ccgcgactgt
     1681 cgtcagcacc ggcaaggcgg ccaccgcggt caccgccgaa atcgcgcagg tcatccgatg
     1741 accgatcgcc tcatcgtt
//
LOCUS       DASWVF010000445         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_88072, whole genome shotgun sequence.
ACCESSION   DASWVF010000445 DASWVF010000000
VERSION     DASWVF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88072"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VGD55_08015"
     CDS             complement(<1..282)
                     /locus_tag="VGD55_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY0870330.1"
                     /translation="MATLVARVVPFTSLQGLGGRRGRVLVERNLMVYRHTWLIIVSGF
                     FEPVFYLFSIGIGIGHLVPKLIFDGHSVGYTAFVAPALLASSAMNGAIYD"
     gene            complement(286..1176)
                     /locus_tag="VGD55_08020"
     CDS             complement(286..1176)
                     /locus_tag="VGD55_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870331.1"
                     /translation="MPFTTAVSWRSRRSCDGARSKTCSCASPAGPWSIEMTSASLPAI
                     PAVRAYRFWLATYRRTWRGTVSTTVVSPVLYLAAMGVGLGSLVHRSPATGGVPYVQFI
                     GPGLLAATAMQVAATESTYSVLGSFKWVRVYHAMAATPLMPLDILIGHLMWIATRIAM
                     TAGIYLGVVAAFGGIRSPLGLLALPACLLIGMAFACPIVAFSTSRENDSGFAALNRFG
                     IIPMFLFSGTFFPVSQLPEVFRWIAYATPLWHGVELVRSLALGRVDVPADLLHAAYLA
                     AWAAVGFVLAARQFQRKLGV"
     gene            complement(1073..>1697)
                     /locus_tag="VGD55_08025"
     CDS             complement(1073..>1697)
                     /locus_tag="VGD55_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870332.1"
                     /translation="YGRYFGLSRAVASERATELLDFVQLLDRATDKVEPLSGGMKRRL
                     TIARSLINRPDVLLLDEPTTGLDPQARHVVWDRLFRLKQQGVTLVLTTHYMDEAEQLC
                     DRLVVMDKGKIAAEGSPRQLISEYSTRDVVEVRFAIGENETGADKLLGLAPRIEVLPD
                     RVLLYTDDGDACVDAVHDSGVLAVSTLVRRSTLEDVFLRLTGRTLVD"
BASE COUNT          358 a          541 c          547 g          251 t
ORIGIN      
        1 gtcatagatg gccccgttca tcgccgacga cgcaagtagc gcgggcgcga cgaacgcggt
       61 gtaccccacg gagtgtccgt cgaagatcag cttgggcacg agatggccga tcccgatgcc
      121 gatcgagaac aagtagaaca ccggctcgaa gaagcccgac acgatgatga gccaggtgtg
      181 ccggtagacc atgagattgc gttcgaccag cacccgcccg cggcggccac cgagcccctg
      241 tagcgatgtg aacggcacga ctcgcgcgac cagagttgcc atgggtcaga ccccgagctt
      301 gcgttggaac tgccgggcgg cgaggacgaa tccgacggcg gcccaggccg cgagatacgc
      361 ggcgtgcaga agatcagcag ggacgtcgac ccgaccgagg gcgagcgagc gcacgagttc
      421 gaccccgtgc cacaacgggg tcgcgtacgc gatccagcgg aaaacctcgg gcaactgcga
      481 gacggggaag aaggtgccag agaacaagaa catcggaatg atgccgaacc tgttgagagc
      541 agcgaatccg ctgtcgttct cccgcgatgt cgagaacgcg acgatcgggc aggcgaacgc
      601 catgccgatc aacaaacacg ccggcaacgc caacaaaccg agcggcgacc tgatgccacc
      661 gaaggcggca acgacgccga ggtagatgcc cgccgtcatc gcgatccgcg tcgcgatcca
      721 catcaggtgg ccgatcaaga tgtcgagggg catcaaggga gtcgcggcca tcgcgtggta
      781 gacccgcacc cacttgaaag atccgagtac cgagtacgtc gattcggtgg ccgcgacctg
      841 catcgcggtc gctgcgagca gccccgggcc gatgaactgc acgtagggca cgccgccagt
      901 cgccgggctt cggtgcacga gtgaccccag gcccacaccc atggccgcga gatacagcac
      961 aggactcacg actgttgtgc tcacggtgcc acgccaggtg cgacggtacg tcgccaacca
     1021 gaagcgatac gcacgtacgg caggaatggc ggggagcgag gccgaggtca tctcaatcga
     1081 ccaaggtccg gccggtgagg cgcaggaaca cgtcttcgag cgtgctccgt cgcacgagcg
     1141 tcgagaccgc caggacaccg ctgtcgtgaa cggcatcgac gcacgcgtcg ccatcgtcgg
     1201 tgtaaagcag tactcggtcg ggcagcacct cgatgcgcgg tgcgagccca aggagcttgt
     1261 ctgcgcctgt ttcgttctcc ccgatggcaa aacgcacctc gacgacgtca cgtgtcgagt
     1321 actcgctgat cagctgcctt ggcgatccct cggctgcgat cttccccttg tccatgacga
     1381 cgaggcggtc acacagctgc tctgcctcgt ccatgtaatg cgtcgtgagc accagcgtca
     1441 cgccttgctg tttcagtcgg aacaacctgt cccacacgac atgcctggcc tgcggatcga
     1501 ggcccgtcgt cggttcgtcg agcaagagca cgtcgggacg gttgatcagt gagcgcgcga
     1561 tcgtaaggcg ccgcttcata ccgcctgaaa gtggttcgac cttgtcggta gcgcgatcga
     1621 gcaactgcac gaagtcgagc agttcagtgg cgcgctcact cgccaccgcg cgtgacaagc
     1681 cgaagtaacg gccgtag
//
LOCUS       DASWVF010000446         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_368340, whole genome shotgun sequence.
ACCESSION   DASWVF010000446 DASWVF010000000
VERSION     DASWVF010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368340"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGD55_08030"
     CDS             <1..429
                     /locus_tag="VGD55_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0870333.1"
                     /translation="AALAADGGFSLIELLVVMIIIGILASIAIPIFLGQRQKSYDAQA
                     KEDIHSAAIAEESYFVEQQTYLNVPTTGTPPALLASSGFRMSSKTIDITVWTYPNGST
                     TGGSTATQQSGSFCVKVTSGSNTPFYYASFGGGLTTTACT"
     gene            462..1121
                     /gene="nucS"
                     /locus_tag="VGD55_08035"
     CDS             462..1121
                     /gene="nucS"
                     /locus_tag="VGD55_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY0870334.1"
                     /translation="MRLVIARCSVDYVGRLTAHLPSAQRLILVKADGSVSIHADDRAY
                     KPLNWMSPPCVLQEAPGVWTVTNKAGEQLVLTIEEMLHDSSHELGVDPGLVKDGVEAH
                     LQVLLAAHPEALGDGMTLVRREYPTPIGPVDLLCRDQLGRHVAVEIKRRGDIDGVEQL
                     TRYLELLNRDALLAPVRGIFAAQEIKPQARVLALDRGIECVTIDYDALRGADDPTARL
                     F"
     gene            complement(1145..3256)
                     /locus_tag="VGD55_08040"
     CDS             complement(1145..3256)
                     /locus_tag="VGD55_08040"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY0870335.1"
                     /translation="MSGSAVNAEVVNARASRISLLVGGAAAQFAVMPLLSGLADAIAM
                     TSFLAIGIATIRLPLPAVAPGTRPGGFKLTMAPRQATALVLIMLGAVLVLGRVNAFDA
                     NSKAAALATLLLVVQVAHCLALQTRREAALGCAIAIVMLSVGAAFAGDVTLLLPVIVA
                     LPAVAVTAALLHRGSLIDAADVTSTGGAGAIVRACLTPVTLATGLGLVVFLLLPNTSH
                     LHTHTHFINSDTATATDAGSGTAASGAGSRSASNPGAGSIDLRARGALSNASVFEVPA
                     TSPAYWQAAVFTEFDGTHWTTSGPLTRWSGSTSAGGTQVAPADLARAANETTQQYTVR
                     VLQSTGLDVVIGPGRPVAYTGTGVVTTDRSGTAHLGGGPSNGGTYAVVSAVPQDQSDA
                     ALLSSTGSEAIDAQSLEIPADLPARVTQLAASLVAGAESRLAAVNAVDDYLRANETYN
                     LNSPEPGRSVDAVDDFLFVSHQGFCEQFATAAVVMLRSQGIPARLVSGYVDGDLTAVP
                     GERVFRGSDAHAWVQVYYPGVGWVNSDPTAGSIAQVSGHSVRQRIGAALTRLWHRVPG
                     GRWGALLGVALAFVLGVGLAEIGRRWLRRRRRFTGVDRGRVGDGPILAAYLRLDVALH
                     GVERAREPSESLGELARRLGGFVATPAEVASAVSFLERESYGVDPPSTAETAEAIGVF
                     DRLRDAVGRELVAVAANAPST"
     gene            complement(3253..>3564)
                     /locus_tag="VGD55_08045"
     CDS             complement(3253..>3564)
                     /locus_tag="VGD55_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870336.1"
                     /translation="VNVIVGFAEAAVPNPEPFIAAKAAGRPLGVTPKGYHRVFATLRA
                     VVPERSTVDVFLDYIGFGGIALLAVGDGLPFEFLSYLQQAATVAGVRVLYVADFLDGA
                     R"
BASE COUNT          704 a         1230 c         1087 g          543 t
ORIGIN      
        1 gctgccctgg cagcggacgg cggcttttcg ctgatcgagc tcctggtcgt catgatcatc
       61 atcggcattc tcgcgtcgat cgccatcccg atctttcttg gccaacggca gaagagctac
      121 gacgcgcagg cgaaggaaga catccacagc gccgccattg ctgaagagag ctatttcgtc
      181 gagcaacaga cctatctgaa cgtgccgacg accggaactc cacccgcatt gctcgccagc
      241 agcggcttcc ggatgtcgag caagaccatc gatatcacgg tctggaccta cccgaacgga
      301 tcaacgaccg gcggctcgac ggccacgcag caaagtgggt cgttctgcgt caaggtcacg
      361 tccggctcga acacgccgtt ctactacgcg agcttcggcg gcggcctcac cacaaccgcc
      421 tgcacgtaac cagccagcac cgcagcggct aatctaagtc tgtgcggctg gtcatagcac
      481 gatgcagcgt cgactacgtc ggccgattga ccgcccatct tccgagtgcg cagcggctga
      541 tcctcgtcaa ggccgacgga tcggtatcga tccacgccga cgaccgtgcg tacaagccgc
      601 tgaactggat gagcccacca tgcgtgcttc aggaagcccc cggcgtctgg accgttacca
      661 acaaggcggg cgagcaactc gtgctgacca tcgaagagat gctgcacgac tcaagccatg
      721 aactcggtgt cgacccgggc ctggtgaagg acggcgtcga ggcgcacctt caagtactgc
      781 tcgctgcaca tcccgaggcg cttggcgacg ggatgacact cgtgcgccgc gaatacccga
      841 ccccgatcgg accggtcgac ctgctgtgcc gcgatcagct tgggcgccat gtcgcggtcg
      901 agatcaaacg gcgtggcgac atagacggcg tcgaacagct cacgcgttac ctcgaactgc
      961 tgaatcgcga tgctctgctg gcaccagtgc gcggcatctt cgctgcgcag gagatcaagc
     1021 cccaagcacg cgtactggca ctcgatcgcg gaatcgaatg cgtgaccatc gactacgacg
     1081 cgctgcgcgg cgccgacgac ccgaccgcac gtctgttcta ggtcgactgt tctaggtcga
     1141 ctttctaagt cgatggcgcg ttagcggcca ccgcgaccag ttcgcgaccg acggcgtcac
     1201 gcaaccggtc gaagactccg atcgcctcgg cggtctcggc ggtcgacggc gggtcgacac
     1261 catagctttc ccgttcgagg aagctgaccg ccgaagcgac ctccgccggc gtcgcgacga
     1321 accccccaag gcggcgagct agttcaccca ggctctcact gggctcgcgc gcacgctcga
     1381 ctccgtgcag ggccacatcg aggcgcaggt acgcggccaa gatgggccca tcgccaacgc
     1441 gcccgcggtc gactcccgta aaccggcgac gacgccgcag ccaccgccgt cctatctctg
     1501 cgagacccac acccaacacg aaggcgagcg cgacgccgag cagcgcgccc caccgaccgc
     1561 cgggaacgcg atgccacaag cgtgtcaacg cagcaccaat gcgctgccgg acagaatgcc
     1621 cgctgacctg cgcgatcgag ccagctgtcg ggtcgctgtt cacccatccg acgccggggt
     1681 agtacacctg cacccacgcg tgcgcgtcgg aaccacggaa gactcgctcg ccgggcacgg
     1741 ctgtgagatc gccgtcgacg tatccgctga cgagccgcgc cggtatcccc tggctcctca
     1801 gcatgacgac ggccgcggtc gcgaattgct cacaaaaccc ttggtgcgaa acaaaaagaa
     1861 agtcatcgac agcatcgacg gatctacccg gttcggggga gttcaggttg tacgtctcat
     1921 tcgcgcgcaa gtagtcgtcg accgcgttca cagcagcgag gcggctctct gcacccgcaa
     1981 ccaaagatgc cgctagctga gtcacgcgag cgggaagatc ggctgggatc tccagcgatt
     2041 gcgcgtcgat agcttcagat ccagtcgacg agagcagcgc ggcgtccgac tgatcctgtg
     2101 gcactgcgga gaccaccgcg tacgtgcccc cattcgatgg cccccctccc aggtgtgctg
     2161 ttccgcttcg atcagtcgtc acgacgcctg tgcccgtgta ggcgaccggc cggcccggcc
     2221 cgatcacgac gtcgagtccg gtgctttgca acactcgcac ggtgtattgc tgcgtggtct
     2281 cgttggcagc ccgagcaaga tccgccggcg cgacctgcgt gccgccggcg ctcgtcgacc
     2341 ccgaccaccg cgtcagcggt ccgctcgtgg tccagtgggt gccgtcgaat tcggtgaaaa
     2401 ccgcagcctg ccagtaagca ggcgacgtcg ccggaacttc gaacaccgac gcgttgctga
     2461 gcgccccacg cgcgcgcagg tcgatcgaac cggctcccgg attgctcgca ctacgcgagc
     2521 ctgcacccga tgccgcggtg cccgaaccgg cgtcggttgc agttgcggtg tcggagttga
     2581 tgaagtgtgt gtgcgtatgc aggtgtgagg tgttgggcag gagcaggaaa accaccagcc
     2641 ccaagcccgt tgccagcgtc accggagtca agcacgcgcg cacgatcgcg cccgctcctc
     2701 ccgtcgacgt cacgtcagcc gcatcgatca gcgacccgcg atgcaacaac gccgccgtca
     2761 cggcaaccgc aggcaacgcg actatcaccg gcagcagcaa ggtcacgtca ccagcgaagg
     2821 cggcgccgac agacaacatc acgatggcga ttgcgcatcc gagcgccgcc tcacgccgtg
     2881 tttgcagagc aagacaatgc gcaacttgga cgacgaggag caacgtcgcg agtgcagcgg
     2941 ccttcgagtt cgcgtcgaac gcattcaccc gcccaagcac gagcacggca ccgagcatga
     3001 tgagcacgag ggcagtcgcc tgacgcggcg ccatcgtcag cttgaagccg ccaggccgcg
     3061 tacccggcgc caccgccggc agcgggagcc ggatcgtcgc aatgccgatc gccaggaacg
     3121 acgtcatcgc gatcgcgtcg gcaagtccgc tcaacaacgg catgacggcg aattgagccg
     3181 ccgcaccgcc gaccaagagc gagatgcggg atgctcgcgc attgaccacc tcggcattca
     3241 ccgctgaccc gctcatcgcg cgccatcgag gaagtcggcg acatagagca cgcggacacc
     3301 agcgacagtc gccgcttgct ggaggtagga caagaactcg aagggcagcc catctccaac
     3361 cgcaagcaac gcgatgccgc cgaatccgat gtagtcgagg aagacatcga ccgtgctgcg
     3421 ctcgggcacc accgcgcgaa gcgttgcgaa tacccggtga taccccttag gggtcactcc
     3481 cagcggtcgc ccagctgcct tcgcggcaat gaacggctcg gggttgggaa ctgccgcctc
     3541 cgcgaaacca acaatgacgt taac
//
LOCUS       DASWVF010000447         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_11879, whole genome shotgun sequence.
ACCESSION   DASWVF010000447 DASWVF010000000
VERSION     DASWVF010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11879"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..703
                     /locus_tag="VGD55_08050"
     CDS             92..703
                     /locus_tag="VGD55_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870337.1"
                     /translation="MPLDEDPESRTMHADLDTAPVYSGTTPYRVRRHTARDCGWGGLV
                     VLIGVLGLLPDSWLTGVVQSKSMFHALFGLLLCGLVAARFQWWLKISPPAHPRDIRQF
                     TRRLSRMVYLVLYLVTGAMEVINFIGARQDGIRPARDFALLKPTSDSVAFLICGLIAL
                     VSIRVLAFWSWRRLVRAGGASVRAGSTWSEPAALGPVDRPPAK"
     gene            complement(705..3929)
                     /locus_tag="VGD55_08055"
     CDS             complement(705..3929)
                     /locus_tag="VGD55_08055"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0870338.1"
                     /translation="MTSDPVPRGADAPLAGLARLAALIVIGTFGSLSGCGSSHPNDLA
                     QLSSIAVGPPDTAVAMGLASQFTATGIMSDGSKRNLSAQVAWSSSNPAIARMGTGGTV
                     ATVRSGTTTIVAALNGMSGSTSLTVTPATLMAIEVTPPSPAFANGLTLQLLATGIYSD
                     NSTHDLTSAVTWSSSTSTVASVSNSPAPAGLAAASSPGSTTIMAALGAVSGTTTLSVT
                     TATLVSIGVTPMNSTLPNGLTTSLKAIGVFTDHSTHDLTAAVTWNSSVPAVATVGVAP
                     ASGLADAVGLGTTQITATLGSVSGTTMMTVSAAELASIGVTPGNPGVALGLKTQLMAI
                     GTYTDNSTQNLTATASWSSSNPALASVSNAAGFEGLATALDPGAVTVTAAMGAIQGST
                     TLTVSPATLVSIAVTPASPGIANGTSRQFTATGTYTDHSTQNLTTTVAWSSSNAGVAA
                     VSNAAGSNGVATGLAQGSATIGAALGSVAGSTSLSVTAATLVSLTVIPANPSIAAGTT
                     RQFTVTGTYTDHSTQDLTSSVSWSSSDTTLVSVSNAAGTNGLAATLAMGTVTITASLG
                     SAVGSTGLTVTPATLVSIAVIPATPSIADGTTQQFAATGAYTDGSTQPLTLAATWRSS
                     DATVATISNAPGTNGLASSVGPGSAAITATVGSIAGSTLLTITPATLVSIAVTPAVPS
                     ITNRSTQQFTATGSYTDGSTQNLTATVTWGSSAPSIATISNAADSNGVATGAASGSTS
                     ISAVLGSISSPAVTLTVTAAPEYAYVANQNDNTVSEFMLGANGGLTLIGAIAAGTEPN
                     AVVVDPTGRYVYVANWSDGTVSEYVVGSDGTLSSIGTAPAGTEPASITVDTTGEYVYV
                     ANLISNTVSQYAIGPDGVLAALGDVPAAGGPISVTVNPTGHYVYAVDETGNAVSAYAI
                     GAGGLLTSLGTAPTGSNPQSIAVDPSGRYAYVANYNDASVSAYTIGSNGTLTAIGTVP
                     SGSCPESVAVDPAGPYVYAENWCSNSVSEYTIGTGGALSAIGTAATGSGPWFITIDPT
                     GSDVYVVNFNDSTVSEFSIATGGALGAIGTVPTGSGPNAITANY"
BASE COUNT          623 a         1261 c         1346 g          735 t
ORIGIN      
        1 cagatttgtt gcagaatcat tgcacccgca atacaccttt gcattacaaa gtaagatgtg
       61 atgtagtgag agcacgcctg tcattagttg catgccgctc gatgaggatc cggaaagtcg
      121 caccatgcat gccgatctcg ataccgctcc agtctactcg ggtactacgc cctaccgcgt
      181 ccggcggcac accgcccgcg actgcggctg gggagggctt gtcgttctca tcggcgtcct
      241 cggcctgctg ccagattctt ggctcacggg ggtggtccaa tcgaagagca tgttccatgc
      301 gctcttcggt ttattactct gcggcctggt ggcggcgcga ttccaatggt ggctgaagat
      361 ctctccaccg gcccacccgc gggacatccg gcagtttacc cgccggttat cgcgcatggt
      421 ttatctggtg ctttacctgg ttaccggtgc catggaggtg atcaacttca tcggcgcgcg
      481 gcaagacggc attcgccccg cgcgagactt cgcgctttta aaacccacct cagacagtgt
      541 ggcgtttttg atctgcggat tgatcgcgct cgtgtcgatt cgtgtgctcg cgttctggag
      601 ctggcggcgc ctggtccgag ctggcggcgc ctcggtccga gctggcagca cttggtccga
      661 gccggcagca cttggtccgg tcgatcggcc gccagcaaaa tagctcagta attcgcggtg
      721 atggcattgg gcccgctgcc cgtcggcacc gtgccgatgg cgccgagcgc cccgcctgtg
      781 gcgatgctga actcggagac cgtgctgtcg ttgaagttca cgacgtacac atcgctcccc
      841 gtgggatcga tggtgatgaa ccaaggcccg cttcccgtcg cggccgtgcc gatcgcgctg
      901 agcgctccgc ccgtgccgat ggtgtattcg gacacgctgt tgctgcacca gttctccgca
      961 tacacgtagg gacccgccgg gtccacggcg accgactccg ggcagctgcc gctcggcact
     1021 gtaccgatcg ccgtcagcgt gccgttgctc ccgatggtgt acgcggacac gctggcatcg
     1081 ttgtagttgg ccacgtaggc atagcgaccg ctcggatcga cggcaatgga ttgcggattg
     1141 ctgcccgtcg gcgcagtacc gagcgaggtg agcagccctc cggcaccgat cgcgtacgcg
     1201 gacactgcat tgccggtttc gtccacggcg tacacgtagt ggcccgtagg gttgaccgtg
     1261 acggagatcg gaccgcccgc cgccggtaca tccccgagag ccgcgagcac gccatccggg
     1321 ccgatcgcgt actgcgagac ggtgttgctg atgagattcg cgacgtacac gtactcgccg
     1381 gtcgtgtcca ccgtgatcga ggcgggctcg gtacccgcag gcgccgtgcc gatgctgctg
     1441 agcgtaccgt cgctccccac gacgtactcg gagaccgtgc catcgctcca gttggcgaca
     1501 tagacatacc ggcccgtggg atcgaccacc acggcgttcg gctcggtccc cgccgcgatc
     1561 gctccgatca gcgtgagccc gccgttcgct ccgagcatga attccgagac ggtgttgtcg
     1621 ttctgattgg cgacgtaggc atactcagga gcagccgtca cggtcagcgt gaccgcgggc
     1681 gagctgatac tgccgagcac ggcgctgatc gaggtcgagc ccgacgcggc gcccgttgcc
     1741 acaccattcg agtctgcggc attgctgatg gtcgcgatcg agggcgccga gctgccccag
     1801 gtcactgtcg cggtcaggtt ctgcgtagag ccgtccgtat agctgcccgt tgcggtgaac
     1861 tgctgggtcg agcggttggt gatgctcggg actgcgggcg tcacggcgat ggagaccaaa
     1921 gtcgcgggcg tgatcgtcag cagcgtcgag cccgcgatgc ttcccacggt cgccgtgatc
     1981 gccgcggagc ccgggcccac gctgctcgcg agaccattcg tgccgggagc gttgctgatg
     2041 gtcgcgacgg tcgcatccga ggagcgccag gtggccgcca gggtcagggg ttgcgtcgag
     2101 ccatcggtgt atgcaccggt tgccgcgaac tgttgagtcg tgccgtcggc gatgctcggc
     2161 gtcgccggta tcaccgcgat cgaaaccaac gtcgcaggag tgaccgtcaa gccggtcgat
     2221 ccgacggcgc tgcccaaact ggccgtgatg gtaacggtcc ccatcgccag cgtcgcggcg
     2281 agaccgttcg tgccggccgc gttgctcacc gagacgagcg tcgtgtccga tgagctccag
     2341 ctcaccgaac tcgtgaggtc ctgggtcgag tggtcggtgt aagtgccggt cactgtaaac
     2401 tgccgcgtcg ttccggcggc gatgctggga ttcgcgggga ttactgtcag cgagaccagt
     2461 gtggcggccg tcacgctgag cgacgtcgag cccgccacgc tgccgagcgc ggcgccgatc
     2521 gtggccgagc cctgggcgag acccgtcgcg acaccgttcg agcccgccgc attgctcacc
     2581 gcggcgacac cggcattgct cgagctccac gcgacggtgg tcgtaaggtt ctgggtcgag
     2641 tggtcggtgt acgtgccggt tgcggtgaac tgccggctcg tgccattcgc gataccgggg
     2701 ctcgcgggcg tcaccgcaat cgagaccagc gtggccggcg acacggtgag cgtggtcgag
     2761 ccctgaatcg cgcccatcgc ggccgtcacc gtgacggcgc ccgggtcgag cgccgtcgcc
     2821 aacccctcga agccggcggc attgctcacg ctcgcgagcg ccggattgga agagctccac
     2881 gacgctgtgg cggtgaggtt ttgggtggaa ttgtcggtat aagtgccgat cgccatgagc
     2941 tgggtcttga gacccagggc aacgcctggg ttgccgggag tgacgccgat cgatgccagc
     3001 tccgcggcgc tgaccgtcat catggtcgtg ccggaaacac tgccgagcgt cgccgtgatc
     3061 tgagtggttc cgaggccgac tgcgtcggcg agcccgctcg cgggggcgac gccgaccgtg
     3121 gccacggcag gcaccgagga gttccaggtc accgcggcgg tgagatcgtg cgtcgaatga
     3181 tcggtgaata cgccgatcgc cttgagggac gtggtcaacc cattcggtag tgtcgagttc
     3241 atcggcgtca cgccgatcga cacgagggtc gccgtggtta ccgagagcgt agtcgttccc
     3301 gacacggcac ccagggctgc catgatcgtg gtggatccgg gactcgatgc cgcggcgagc
     3361 ccggccgggg ccggtgagtt gctgaccgaa gcgaccgtcg aagtcgagga gctccaggtc
     3421 actgcactgg tgagatcgtg cgtcgagtta tcgctgtaga tgccggtggc caggagctgg
     3481 agcgtcagtc cgttcgcgaa cgccggactc ggcggggtga cctcgatcgc catcagcgtg
     3541 gcgggggtca ccgtcaaact cgtcgatccg gacatgccgt tcagggccgc gacgatggtc
     3601 gtggtaccgg atcgcaccgt ggccacggtg ccgcccgtgc ccatcctcgc aatagcagga
     3661 ttgctcgagc tccaggctac ctgcgccgag agattgcgct tcgagccgtc gctcatgatt
     3721 cccgtggcgg tgaattggct cgcgagcccc atcgcgaccg cggtgtcggg cggcccgact
     3781 gcaatcgagc tcagctgcgc aagatcgttc ggatgcgacg atccgcaacc ggacagcgat
     3841 ccgaaagtgc cgattacgat cagcgcagcc agccgggcga gtccggcgag gggcgcatcg
     3901 gcgcccctcg gcacagggtc cgaggtcatt cattgactcc aatcgcactg cgttcattgc
     3961 aggcg
//
LOCUS       DASWVF010000448         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_241025, whole genome shotgun sequence.
ACCESSION   DASWVF010000448 DASWVF010000000
VERSION     DASWVF010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241025"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VGD55_08060"
     CDS             <1..1460
                     /locus_tag="VGD55_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870339.1"
                     /translation="QPEESARALRRHDGRWWVSLDRWWREPALAIDDTVWAWAIDSVD
                     GTVYAGSNHGVFKSIDGGNTFALTGSPGNGIVYSLTKSGRDLYAGTGGGGFSVSSDGG
                     ATWTNTDIAKGIGLSLNTDTAGSVYAGTTFEGAFVKPVQSNYYHPAQWRRIAWDNIRN
                     CNCQNGHAIAIDPNDDEHIFFTTNDGGLLVSKDGGGSWSDLSANGLGARAPRSVGFDP
                     SNPKRIYVGGYTGNGFWRTSNGGKTWTRSFFGPPNVYPTQPVVDPIDGTVYVATLSMG
                     VYKSIDFGANFTRIDQAPAAAPGVYLNIGGRGLEMDPHDRRTLFLAARGKGTWKTIDS
                     GVSWVQVDPTFALSVTVDPSNSNVVYAAGDPSSGDPGVIKSIDGGITFFPSGNGFPGT
                     SQTARTGAVQVNPRDPNQLFVGFEGDGVYTSSDAGATWVGANTCLTNANVQGITMDLS
                     SPRTLYASTFGSVFKTTGYHIKYGPCEPRLPGYDR"
BASE COUNT          403 a          611 c          600 g          372 t
ORIGIN      
        1 ctcaacccga agaatccgct cgagctcttc gtcggcacga cgggcggtgg tgggtatcac
       61 tcgaccgatg gtggcgcgag ccggcgcttg cgatcgacga cacggtgtgg gcatgggcga
      121 tcgactcggt cgacggcacg gtctacgccg gctcgaacca cggcgtcttc aagagcatcg
      181 acggcggcaa cacgtttgcg ctcacgggct cgccgggaaa tggcatcgtc tattcgctca
      241 cgaagagcgg tcgtgatctc tacgcgggca ccggcggcgg tggcttctcg gttagcagcg
      301 atggtggcgc gacgtggacg aacaccgata ttgcaaaagg catcggtctc tcgctcaaca
      361 ccgacactgc gggcagcgtg tacgcgggaa ccaccttcga gggcgcattc gtgaagcctg
      421 tccagagcaa ctactaccac cccgcgcaat ggcgtcggat cgcgtgggac aacatccgca
      481 actgcaattg ccaaaacggg catgcgatcg cgatcgaccc gaacgacgac gagcacatct
      541 tcttcaccac caacgacggt ggtctgctcg tcagcaaaga cggcggcggc agctggtcgg
      601 acctctccgc caacggcctc ggtgcgcgcg ctccgcgtag tgtcggcttc gacccgagca
      661 acccgaaacg catctatgtc ggtggttaca ctggcaacgg cttctggcgc acgtcaaatg
      721 gcggcaagac ctggacgcgg agttttttcg gtccgcccaa cgtctacccg acccagccgg
      781 tcgtcgatcc gatcgacggg actgtctacg tcgcaacgct cagcatgggc gtctacaaga
      841 gcatcgactt cggcgccaac tttacgcgta tcgaccaagc accggccgca gcaccgggcg
      901 tctatctcaa tatcggtggt cgtggcctcg agatggatcc gcatgaccgc cgcacgcttt
      961 tcctggcggc tcgtggcaag gggacgtgga agactatcga tagtggtgtg tcgtgggtcc
     1021 aggtcgaccc aacctttgct ctctccgtga cagtcgaccc gagcaactcg aacgtcgtct
     1081 acgccgccgg cgacccgtcc tcgggggacc cgggcgtcat caagagcatc gacggtggta
     1141 tcaccttctt tccgtcgggt aacggcttcc cgggcactag ccaaaccgcg cgcaccggcg
     1201 cggtgcaggt taacccgagg gatccgaacc agcttttcgt cggctttgaa ggcgacggcg
     1261 tctatacctc gagcgacgcg ggcgcaacgt gggtcggcgc gaacacgtgc ctcacgaacg
     1321 cgaatgttca gggcatcacg atggatctct cctcaccgag gaccctctac gcctctacct
     1381 tcggctcggt cttcaagacc actggctacc acatcaagta cggcccgtgc gagccgcgcc
     1441 tgcccggcta cgatcgctag gacgaccgcg ggcagctcaa gttcgcaagg caaactagtc
     1501 cccacacctc gacacctcac tcccgtgcgc ggctgctcaa tccggccgga agcggtgtcg
     1561 atgagatcga ctctgcggct acgcggggcg gctaaaaatg gtgttcttac actaggccga
     1621 tctggcagcg agaggacacg gcgacgcaat agccaacatt gttcgtagcg attgctgtga
     1681 ttgctggccg ctcaaccacg tcgggcgtcg cgcccacgga atgcactcta atgatttata
     1741 attcgtggta gggaacggac gaactattga tttgggtgac cttatgcggt ggagggagca
     1801 tggcacaagt gacgtatgaa aaaggtgatc actgcacttc tggtgatcga tccgtacaat
     1861 gacttcattt ccgaaggggg caaggtatgg gatcgcctga aggctgttgc agaagaaaat
     1921 ggttgtgttc cccatcagta cctcaacaat ctgatcgagc aggatcatcg cgctataaag
     1981 cgccga
//
LOCUS       DASWVF010000449         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_368396, whole genome shotgun sequence.
ACCESSION   DASWVF010000449 DASWVF010000000
VERSION     DASWVF010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368396"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VGD55_08065"
     CDS             <1..1125
                     /locus_tag="VGD55_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870340.1"
                     /translation="DGLLLHAGVGAEAGYDTNVYYDSSKPIGSSIFRVMPFAEITNAT
                     RNGAVGTQLTFDARAGLQYRHYGNSEIRSAYADAWNPNAGLSLSYGGAQFGFGVADVF
                     ARIEDPPYATGGIPQSGAIERTNNQASVEGRWAPGGGRLTTTLRYTNMVDVYGGLYSY
                     ADSVTHTLMLDVSWKWLPKTAVFANFQQSYLDYYNHGPTAKSDAYPLTATVGARGLLT
                     AKTSVLLALGYTNSFYQTGGSTSGVWGQTYANLAFTVLPTQLSRVVVGFRHDFLNAVI
                     SNFAYEDTVYAAYVQQLVGRLGLDLSGRYARLDYGGAFVDPAQSGRVDNLFQIGATLD
                     YFLRNWAYVGAGYSLLDNRSNIATDSYLKQQVFARLGVTY"
     gene            1138..>1511
                     /locus_tag="VGD55_08070"
     CDS             1138..>1511
                     /locus_tag="VGD55_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0870341.1"
                     /translation="MSAGSFRRDQLLLVAAVVGFIGCYPHGKPPANVVDQVVAAEDRV
                     GIDDTFDVRVYGEPELSGMFRVATDGTVDYPLAGRLQVAGIRTGDIQRLIVDKLKDRY
                     LKDPQVIVTIKDRNSQKISVLG"
BASE COUNT          275 a          479 c          469 g          288 t
ORIGIN      
        1 gacgggttgc tcctgcatgc aggcgtcggg gcggaggcgg ggtacgacac gaacgtctac
       61 tacgactcat cgaagccgat cggctcgtcg attttccggg tgatgccgtt cgcggagatc
      121 acgaacgcca cgcggaatgg cgccgtcggc acgcaactga ccttcgacgc tcgggcaggt
      181 ctgcaatacc gacactacgg gaactcggag attcgttcgg cttacgcgga cgcctggaac
      241 ccgaacgccg gcttgtccct cagttacggc ggcgctcaat tcgggttcgg cgtcgcggac
      301 gtatttgccc gcatcgaaga cccgccgtac gcgacgggtg gcatcccaca gagtggcgcc
      361 atcgagcgca cgaacaacca ggcgtcggtg gaaggccgct gggcgcctgg aggcgggcgc
      421 ctcaccacga ccctccgcta cacgaacatg gtcgacgttt acggcggctt gtactcctac
      481 gcggactcgg tgacgcacac gctgatgctc gacgtctcgt ggaagtggct tccgaagacc
      541 gcggtgttcg cgaacttcca gcagagctac ctcgattact acaaccacgg accgactgcg
      601 aagtcggatg cgtatccgct gacggcgact gtcggcgcgc gcggtctctt gaccgccaag
      661 acctcggttc tgctcgctct cggctatacg aattccttct atcagacagg cggctccacg
      721 agcggcgttt ggggccaaac ctacgccaac ctggcgttca ccgtgttacc aacgcaattg
      781 agtcgagtcg tggttggttt tcgtcacgat ttcctcaacg cggtcatcag caacttcgcg
      841 tacgaggaca cggtgtatgc cgcctacgtt cagcagctgg tcggacggtt gggcctggac
      901 ctctccggtc gctacgcacg gcttgactac ggcggggcgt tcgtcgaccc ggctcagagc
      961 ggtcgcgtgg acaatctctt ccaaattgga gcgacgctcg actacttcct gcggaactgg
     1021 gcgtacgttg gcgccggcta ctcgttgctg gacaaccgca gcaacatcgc caccgacagc
     1081 tacctgaagc aacaggtgtt tgcgcggctc ggcgtcacgt actagtctcc accgctcatg
     1141 tcagcaggtt cgtttcgcag agaccagctc ttgctggttg ctgcggtggt cggcttcatc
     1201 gggtgttacc cccacggcaa gcccccggcc aacgtcgtcg accaggtcgt cgcagccgaa
     1261 gaccgcgtcg gtatcgacga cacgttcgac gtgcgcgtct atggcgagcc cgagctctcg
     1321 ggcatgttcc gcgtggccac agacggcacc gtcgactatc cactggccgg ccggctgcag
     1381 gtggcgggta tacgtacggg cgacatccag cgactgatcg tcgacaagct gaaggatcgg
     1441 tacctgaagg atccgcaggt catcgtcacg atcaaggacc ggaacagcca gaagatctcg
     1501 gtgcttggtc a
//
LOCUS       DASWVF010000450         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_164422, whole genome shotgun sequence.
ACCESSION   DASWVF010000450 DASWVF010000000
VERSION     DASWVF010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164422"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VGD55_08075"
     CDS             complement(<1..1000)
                     /locus_tag="VGD55_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0870342.1"
                     /translation="MRNRDAQGMLVGVPMPTWSRNPPTWWRTPPAWLIAAVAGVMCIA
                     TRALPWHEAVATTKRVGPILVFLVAITVVAELADAAQVFDVAATRAAKLGRGHTLGLF
                     GLIVVLGSFTTIVLSLDTTAVLLTPVVLALASQLRISVLPFAMAAVWLANTASLLLPV
                     SNLTNLLAVNRLHLSAVSFAAHVWLPAIASIAVTAVVLGVAYRRDLRGRYVVPGAFAI
                     ADPVSFRVCATMCVLIAPFFVIGLPVWLPAMVAGGVLLVLFAFRQPRRIGIALLPWRL
                     VLLVQGLFLVVATLGAHGLDRVLGDAAGHSDSALGVLRTAGVGAGASNIANNLPSYL"
     gene            1048..2505
                     /locus_tag="VGD55_08080"
     CDS             1048..2505
                     /locus_tag="VGD55_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY0870343.1"
                     /translation="MPHRRVRLSVDGTALREGFAAIRTELGVPANFPPEVLAAADRAA
                     AAWSSTTSTLPDRTDIAFITIDPPGSMDLDQAMHISRLTPGTPGKAGYRVRYAIADVA
                     AYVAPGDPVDVEAHVRGETLYSPDTRTPLHPPSIGEAAASLLQDQTRPSLVWTIDLDA
                     DGRQVVVDVKRALVRSRARLNYTQAQQAIDAKTGEGAADGTLALLAEVGRLRHALEVE
                     RGAVSLRLPEQEVDADGKGYRLRYRAPLPCEDWNAQISLLTGMAAAGIMLDARVGILR
                     TLPKPDGGAIGMLRRSAHALDVHWPHSVKYPAFVRGLDPATDTGAALLELSTRLLRGA
                     GYTAFDGTPPAEPNHSAVAAPYAHATAPLRRLVDRYVGELCVAVCAHLDVPPWIREAL
                     PALADVMAESGHRAHALEHACVDLVETVLMASHVGETFEGTIVETGKHGGTVQLRTPP
                     VRARCNAEGLTLGERIEVRLETADPATHTLLFARP"
     gene            2532..2945
                     /gene="rnpB"
                     /locus_tag="VGD55_08085"
     ncRNA           2532..2945
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGD55_08085"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          541 a         1032 c         1025 g          453 t
ORIGIN      
        1 cgagatagct cggcaggttg ttcgcgatat tgctcgcacc agcgccgacg cccgcagtac
       61 gcagcacgcc gagtgcactg tcgctgtgac ccgccgcatc gccgaggacc cggtcgagtc
      121 cgtgcgcgcc caacgttgcg accacgagga acaggccctg cacgagcaac acgagccgcc
      181 atggcaacag cgcgatcccg atacgtcgcg gttgccgaaa cgcaaacagc acgagcagga
      241 cgccgccggc aaccatcgcc ggtaaccaca ccggtagccc gatcacgaag aacggcgcga
      301 tgagcacgca cattgtcgcg cagacccgaa agctcacggg gtcggcgatc gcgaacgcac
      361 ccggcacgac gtagcgtccg cgcaggtcgc gtcgataggc aacaccgagc accaccgccg
      421 tcaccgcgat cgacgcgatc gcgggcaacc acacatgagc cgcgaacgag actgccgaca
      481 ggtgcagtcg attgactgct agtagattcg tcaggttcga caccgggagc aacaggcttg
      541 cggtgttggc cagccacacc gcggccatcg cgaacggcag caccgagatc cgcagctgcg
      601 atgcgagcgc gagcacaacc ggagtcagca gcaccgcagt ggtgtcgagg ctgagcacga
      661 tcgtcgtgaa gctgccgagc acgacgatga gcccgaacaa cccgagcgtg tgaccccggc
      721 cgagtttggc ggcacgggtc gcggcgacgt cgaacacctg cgcggcgtcg gcgagttcgg
      781 cgaccacggt gatggcgacc aggaatacga ggatcggtcc gactcgcttc gtcgtcgcga
      841 ctgcttcgtg ccacggcagc gcgcgggtcg cgatgcacat cacgccggcg acggcggcga
      901 tcagccacgc cggcggagtg cgccaccacg ttggcggatt gcgcgaccac gtcggcatcg
      961 gcacaccgac gagcatgccc tgtgcgtcgc ggtttctcac atcggcctgc tccttacacg
     1021 gggcgggcct tcacatacgg tgtcggcatg ccgcatcgca gggtccgtct cagtgttgac
     1081 ggcacggcat tgcgtgaggg cttcgccgcg atccgcaccg agctcggagt ccccgccaac
     1141 ttcccgcccg aggtattggc ggcggccgat cgtgcggcgg ccgcctggag ctcaaccacc
     1201 tcaaccctgc ctgaccggac cgacattgcc ttcatcacga tcgacccacc aggttcgatg
     1261 gacctcgacc aggcgatgca catctcgcgc ctcacccctg gcacgccggg caaggccggc
     1321 taccgcgtgc ggtacgcgat cgccgacgtt gccgcgtacg tcgcgccggg cgacccggtc
     1381 gatgtcgagg cgcacgtgcg cggcgaaacc ctgtacagcc ccgacaccag gaccccactc
     1441 cacccgccgt cgatcggtga agctgccgcg agcctgctcc aggatcaaac aagaccctcg
     1501 ctcgtgtgga cgatcgatct cgacgccgac ggtcgccagg tagtggtcga cgtgaagcga
     1561 gcactcgtgc ggtcgcgcgc ccgcctcaac tacacccagg cccagcaggc gatcgacgcg
     1621 aagacgggcg aaggtgccgc cgacggaacc ctcgcgttgc tcgccgaggt cggtcggttg
     1681 cggcacgcgc ttgaggtcga gcgcggcgcc gtcagcctac gcttgcctga acaagaggtg
     1741 gacgccgacg ggaagggtta tcggttgcgt taccgcgccc cgctgccgtg cgaggactgg
     1801 aacgcgcaga tctcgctact caccggaatg gcggccgccg gcatcatgct cgacgcccgc
     1861 gtcggcatct tgcggacgtt gccgaaaccc gatgggggag ccatcggaat gctccgccgc
     1921 agcgcccacg ccctcgacgt ccattggccg cattccgtta agtacccggc gtttgtccgc
     1981 ggtctcgatc cagcaaccga cacgggcgcg gcgttgctcg agctatcgac acggttgctg
     2041 cgcggagcgg gctacaccgc gttcgacgga acgccgccgg cggaacccaa tcacagcgcg
     2101 gtcgcggctc cgtacgcaca tgcgacggca ccattgcggc ggcttgtcga ccggtacgtg
     2161 ggcgaactgt gcgtggcagt ttgtgcgcac ctcgacgtcc cgccgtggat tcgtgaggct
     2221 ctacctgcac ttgctgatgt catggctgag tcggggcatc gcgcccacgc acttgagcac
     2281 gcgtgcgtcg acctcgtcga aacggtgctg atggcgtcgc atgtcggaga gacgtttgag
     2341 ggcacgatcg tggagaccgg caagcacggt ggcacggtgc aattgcggac gccgccggtg
     2401 cgcgcccgat gcaacgcgga gggcttgaca ctgggggagc gcatcgaggt ccggctcgag
     2461 accgccgatc cggcgaccca cacgttgctg ttcgcgcggc cctgaccgtc tagagttgct
     2521 gctacggcgg acgagtcggc cgggcgaccg cgttgcgtac cttcgggtgc gtgccgagga
     2581 aggtccgggc tccacagggc aaggtggtgg gtaacgccca cccggggtga cccgcgggac
     2641 agtgccacag aaaacagacc gctgttgggc tcacggtttt ggctttgggc ttggcagtaa
     2701 gggtgaaacg gtggtgtaag agaccaccag cgtgcgaggt gactcgcgcg gcttggtaaa
     2761 ccccacctgg agcaaggtca agaggtcacg cttcggtgtg actgcgcagg cgtttgaggg
     2821 ctgcccgccc gagcctgcgg gtagaccgct ggaggctgtc ggcaacggca gtcgtagatg
     2881 gatggtcgcc cgcccgaacc gcaaggttca ggcgacagaa cccggcctat aggccgactc
     2941 gtccgccctg acctccgtcg tcacagtccc gcgctgaatc cggcatcacg ctgcgtctgg
     3001 tcccggaccg cggtcgcgcc ggagctcaag ccgatcggct ggacgaagac g
//
LOCUS       DASWVF010000451         5896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_215869, whole genome shotgun sequence.
ACCESSION   DASWVF010000451 DASWVF010000000
VERSION     DASWVF010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215869"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGD55_08090"
     CDS             complement(<1..336)
                     /locus_tag="VGD55_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEY0870344.1"
                     /translation="MPSSLRACIDSALAGTTGASLTRNVERLIDSYRSGETPLTPVLA
                     SQDDVLAYAAYRMPATYAVVHAALQRLHTAVPELQPLTLVDFGAGTGAATWAATVVWP
                     GIEKATLLEQ"
     gene            complement(363..719)
                     /locus_tag="VGD55_08095"
     CDS             complement(363..719)
                     /locus_tag="VGD55_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0870345.1"
                     /translation="MAKFMILIYGDADQWAAMSPAEQRVHNAAHEAFAKAAGTRIVGG
                     EELELAPRARTVRKGPAGRVITTDGPFLETKEALGGFYLLEAADVDEAIKLASKLPEV
                     AAGHSGVEIRPVVDHG"
     gene            920..1915
                     /locus_tag="VGD55_08100"
     CDS             920..1915
                     /locus_tag="VGD55_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0870346.1"
                     /translation="MSIAADQPIRWGIISTGGIASAFTGDLALLPDAKAVAVGSRSQS
                     SADAFGDRFVIARRYPTYEALVADPEVDAVYVATPHPGHHAAALLAINAGKAVLVEKP
                     FTINAGEARDLIAAAQAQGTFLMEAMWTRFLPHVIRIREIIATGRLGEIRSVVADHGQ
                     WFPKNPDHRIYAPVLGGGALLDLGIYPLSFASMILGPPTKVTAVSDVAFTGVDAQTSV
                     ILQYGDGRQSISFTTLETRTANTAVINGTEARIEVDGVFYSPNSFSVITRDGEVERFD
                     IPHEGHGLRHQAAEVGRCLRAGLTESTILTLAETLSIMETLDEVRRQIGLTYPSE"
     gene            complement(1857..2126)
                     /locus_tag="VGD55_08105"
     CDS             complement(1857..2126)
                     /locus_tag="VGD55_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870347.1"
                     /translation="MKRHEPDWTSLIAGVTFCAIAVAYLGGEITHRSLELRWVLPMLL
                     IGLGVAGLAGTLVRARRLSEPAPAEMPTPTDTSDLSDDEPRPASP"
     gene            complement(2123..3544)
                     /locus_tag="VGD55_08110"
     CDS             complement(2123..3544)
                     /locus_tag="VGD55_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY0870348.1"
                     /translation="MTQDFSSSAGTPPNPAPDPSGSTSGRHDLRTLRRSRDDRMIGGV
                     CGGLGRYAGIDPIIFRIVLAALAVFGGVGLLLYALGWLLIPEEGSDVSELHRLLRGHG
                     SPLPIIAAIVIGFLGLLAFVDVVNHRPAGQFALLLIVLAVVAVIALTRRAGNEPPTAS
                     ATPPTSTPPMYTPPPTPQYAAAQSAAPQYSPPPQSAPFAAPPQSAPFAAPPHYTAPLG
                     PPAGFSPSRPMYTPPPGYVPPPPKPPRPPSILGPLAISVGIVVIGVLFALDASHTIEI
                     SAQTIFAASLLTVGLALVVGTWIGRGRVLIAFGVVLTIALALAAAIDVPLRGGIGSRD
                     STPTSIAEIPSAYHLGIGRQSIDLTGLQLAGKTVHVTAEVGIGRLWVNVPANVKVVVR
                     GRAGTGSVDVNGSQVGGTHLDRTLLIPTSGTPTPGEIDLDLRVGFGEVSVVQGVALDA
                     PVTPPVPALATPTAPAAPEVIQP"
     gene            3659..4858
                     /locus_tag="VGD55_08115"
     CDS             3659..4858
                     /locus_tag="VGD55_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY0870349.1"
                     /translation="MPPKLTRASPGQPGQLVAGVARGLADHLRVNVALVRVAFIAMSF
                     AAGAGIVMYAAFWVFVPMTPAAPGQDRVAPANRLDGRGQYLAIGALTIGGVLAIQSLG
                     LGLPSELLWPLALTGFGAMVLWRQADEAQRTRWRKAATGRDKPPAVATTIAGACLVIA
                     GAASFLAYRGQLSQVRNGLLAAVVMVAGIAVITGPWWLGTLHELSAERRARIREQERA
                     EIAAHLHDSVLHTLALIQRHVDDPREVQRLVRGQERELRTWLYRPQPDRELDFGADLE
                     RIVAEVEDNHGVPIELVVVGERRLDDGLSATLQAAREAMVNAAKHAGTATISVYAEVE
                     PTKVTVFVRDRGRGFDVAEVPEDRLGVKESIVGRMERHGGHAYVRTAPGMGTEVQLEM
                     SSLMARA"
     gene            4855..5502
                     /locus_tag="VGD55_08120"
     CDS             4855..5502
                     /locus_tag="VGD55_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870350.1"
                     /translation="MSSPVKIRVVIVDDHRLFRAGVRAELGDDIDVVGEAEDVPSAVS
                     VIAQAVPDVVLLDVHLPGGGGVEVLRQIRPVLPDVRFLAVSVSDAAEDVIGTIRAGAC
                     GYVTKTISGGDLADAVTRVAEGDVVFSPRLAGFVLDAFAATDMPAIDPELDALTQRER
                     EVLRLIARGYAYKEIAKELFISVKTVETHVSSVLRKLQLSNRHELTRWATDRRLV"
     gene            5513..>5896
                     /locus_tag="VGD55_08125"
     CDS             5513..>5896
                     /locus_tag="VGD55_08125"
                     /inference="COORDINATES: protein motif:HMM:NF039724.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6314 family protein"
                     /protein_id="HEY0870351.1"
                     /translation="MHAVADLAAYLLGRWSIEREIVDAHHTGFFRGNAEFTGDDGVVD
                     WIEAGQLQWAGETHQAGRRLLLAVRPDSLSVADVAFDDGRFFHRLDLSRGCDTFVHGC
                     APDTYIGSWAVDDSDQFTIRWTIEGP"
BASE COUNT          967 a         1920 c         2018 g          991 t
ORIGIN      
        1 ttgctcgagc aacgtcgcct tctcgatgcc cggccacaca acggttgctg cccaggtcgc
       61 cgcacccgta cctgcgccaa aatcgacaag cgttaaaggc tgaagctccg ggacggcggt
      121 gtgcagccgt tgtagcgcgg cgtgcaccac cgcgtaggtg gcgggcatcc ggtaagcggc
      181 gtaggcgagc acgtcgtctt ggctcgcaag caccggggtg agaggcgttt caccggatcg
      241 gtagctgtcg atgaggcgct cgacgttgcg agtcaagctt gcgcccgtcg tgcctgcgag
      301 cgcgctgtcg atacacgcgc gcaggctcga cggcatggca cgaccctatg cgggtagtcg
      361 cgtcacccgt ggtcgacgac cggccggatc tccaccccgc tgtgcccagc cgccacctcg
      421 ggtagcttgc tcgccagctt gatcgcctcg tcgacatcgg cggcctcgag caggtaaaag
      481 ccacccagcg cttctttggt ctcaaggaac ggtccgtcgg ttgtgatgac gcggccggcc
      541 ggacccttcc gcactgtccg cgcgcgcggt gcgagctcga gttcttcgcc tccgacgatc
      601 cgcgtgcctg cagccttggc gaacgcttcg tgcgcggcgt tgtgcacgcg ctgttccgct
      661 ggggacatcg cggcccactg gtcggcatcg ccatagatca ggatcatgaa cttcgccatc
      721 tcgtgtccgt ccgatcgtga tgagcctgtc aggcccggtt gcttctacga cgactcgcgc
      781 gggtcgcgca tcgacaaaac ggcgacggtc aatttcgagc agcctagggg tatccgcggg
      841 cggagccgtc gagcccgagt cgtaaggttc cgaccagaac gccagctcat cgcacaccgc
      901 cgacatcagg gagacgtacg tgtcgatcgc cgctgaccag ccgattcggt gggggatcat
      961 cagcacagga ggtattgcct cggccttcac cggtgacctc gcgttgctgc ccgacgccaa
     1021 ggcagtcgcc gtcggatcac gcagccagag ttcggccgac gcgttcggcg accgcttcgt
     1081 catcgcgcgc cgctatccca cgtacgaggc gctcgtcgcc gatcccgagg tcgacgccgt
     1141 ctacgtcgcc accccgcacc cagggcatca cgcggctgct ctcctcgcga tcaacgccgg
     1201 caaggcggtg ctggtcgaga agccgttcac gatcaacgcg ggcgaagcgc gcgacctgat
     1261 cgccgccgcg caggcacagg gcacctttct gatggaggcg atgtggacca ggttcttgcc
     1321 ccacgtcatc cgcatccgcg agatcatcgc caccggtcga ctcggtgaga tccgctcggt
     1381 cgtcgctgat cacggtcagt ggttcccgaa aaatcccgac caccgcatct acgcacctgt
     1441 cctcggtggg ggtgcgttgc tcgacctcgg gatctatccg ctgtcgttcg cttcgatgat
     1501 tctcgggccg ccgaccaagg tgaccgcggt ctccgatgtg gccttcacgg gcgtcgatgc
     1561 gcagacctcg gtcatcttgc agtacgggga cggcaggcag tcgatctcgt tcaccacgct
     1621 ggagactcgc acggcaaata ccgcagtgat caacggcacc gaggctcgga tcgaagtcga
     1681 cggcgtcttc tactcgccaa actcgttcag cgtcatcacc cgcgacggcg aggtcgagcg
     1741 attcgacatc ccccacgagg gccacggtct gcgacaccaa gctgccgaag ttggccgctg
     1801 cctgcgcgcc ggcctcacgg aaagtacgat tctgacgctg gccgagacgc tgtcgatcat
     1861 ggagacgctg gacgaggttc gtcgtcagat aggtctgacg tatccgtcgg agtaggcatt
     1921 tctgccggtg ccggctctga cagtcgccgc gctcgcacca acgtcccggc gagccctgcg
     1981 acgccgagac cgatcagcag catcgggagc acccagcgca gttcgagact gcgatgggtg
     2041 atctcgccac cgaggtaagc gacagcgatc gcgcagaacg tgacacctgc gatcaacgag
     2101 gtccaatcag gttcgtggcg cttcatggct gaataacctc cggggcggcg ggcgcagtcg
     2161 gcgtggcgag tgcaggcacg ggcggcgtca cgggagcgtc gagcgcgacg ccttgaacaa
     2221 cgctgacctc accgaagccg acgcgcaggt cgagatcgat ttcgcccggc gtcggcgtcc
     2281 cgctcgtcgg tatgagcagc gtgcgatcga ggtgcgtgcc acccacctgg ctgccgttga
     2341 catcgacgct gccggtgcca gcgcgaccgc gcacgacgac cttgacattc gcgggcacgt
     2401 tgacccagag ccgacctatc ccgacctcgg cggtgacgtg cacggtcttg ccggcaagtt
     2461 gcaaccccgt gaggtcgatc gattgcctgc cgatcccgag gtgatacgcc gacggaatct
     2521 cggcgatcga cgtcggtgtc gagtcacggc taccgatacc accccgcaac gggacgtcga
     2581 tcgctgcggc cagcgccaac gcgatcgtga gaaccacacc gaacgcaatg agcacgcggc
     2641 cccgcccgat ccaggtgccg acgacgaggg ccagtccgac ggtgagcagc gatgccgcga
     2701 agattgtctg ggcgctgatc tcgatcgtgt gtgacgcgtc gagcgcgaac agcaccccga
     2761 tgacgacgat gcccacgctg atcgccagcg gcccaaggat cgagggcggc cgcggtggct
     2821 tcggcggtgg cgggacatat cccggtgggg gcgtgtacat cgggcgggac ggcgagaagc
     2881 cggcgggcgg tccgagcggc gccgtgtagt gcggcggagc cgcgaatgga gccgactgcg
     2941 gcggagccgc gaagggggcc gactgcggcg gcggtgagta ttggggtgct gcggactggg
     3001 cggccgcgta ctgcggggtg ggaggcggcg tgtacatggg tggcgtggac gtcggaggcg
     3061 tcgcggacgc cgttggcggc tcatttccgg ctcgacgggt gagcgcgatc accgcgacga
     3121 ccgcgagcac gatgagaagc agcgcgaact ggcccgccgg cctgtggttg acgacatcga
     3181 cgaaggcaag gaggccaagg aacccgatca cgatggccgc gatgatcggc agcggtgaac
     3241 cgtggccccg gaggagtcga tgcaactcgg aaacatcgct gccttcctcg ggtatgagca
     3301 accagccgag ggcgtacaga agaaggccaa cgccgccgaa gacggcgagc gcggcgagga
     3361 cgatccggaa gatgatcggg tcgatgcctg cgtaccggcc gaggcctccg cagaccccgc
     3421 cgatcatgcg gtcgtcgcgg ctgcgccgca gggtgcgtag gtcatgccgg ccggaggtcg
     3481 agccgctggg gtctggtgcg gggttcggcg gcgtgcccgc agaactggag aagtcttgtg
     3541 tcatagcgac gatcctggcg tcgcgccgct cctgcgccca tcgggaacta ccctgactcg
     3601 accctgactt tgtgctggca acggcgctcc ggggcgcggg acgtgtgacg atcgtggggt
     3661 gccaccaaaa ctgacgcgtg cgagccctgg ccaaccgggc caactggtcg ccggcgtggc
     3721 ccgtggcttg gcagatcacc tgcgggtcaa cgtcgccctc gtgcgagtcg ccttcatcgc
     3781 gatgtcgttc gccgccgggg cgggcatcgt gatgtacgcc gcgttctggg tcttcgtccc
     3841 catgacaccc gccgcaccgg gccaggatcg ggtcgcgccc gcgaacaggt tggacggccg
     3901 aggtcagtac ctcgcgatcg gggcgctcac gatcggcggt gtcctggcca tccagtcgtt
     3961 gggccttggg cttcccagtg aactgctgtg gccactcgcg ctgacggggt tcggcgcgat
     4021 ggtcctgtgg cgccaggccg acgaggctca acgaacccgc tggcgcaagg ctgcgaccgg
     4081 acgcgacaag ccgccagctg tcgcgaccac catcgccgga gcctgcctgg tcatcgccgg
     4141 cgcggccagc ttcctcgcct atcgcgggca actgagccag gtgcgcaacg ggttgctcgc
     4201 agctgtcgtg atggtggctg gcattgccgt catcactgga ccgtggtggc tcggcacatt
     4261 gcacgaactc tctgccgagc ggcgcgcgcg gatccgtgag caggagcggg cagagatcgc
     4321 ggcccacctg cacgactcgg tgctgcacac cctggcgctc attcagcgcc atgtcgacga
     4381 cccgcgcgaa gtgcagcgcc tggtgcgcgg gcaagaacgc gagttgcgca cctggttgta
     4441 ccgcccgcag cccgaccgcg agctcgactt cggcgcggac ctagagcgca tcgtggccga
     4501 ggtcgaagac aatcacggag tgccgatcga actcgtcgtc gtgggtgagc ggcggctcga
     4561 cgacggtttg tcggcgacgc tgcaagctgc tcgggaggcg atggtgaacg cggcgaagca
     4621 cgcaggcacc gccaccatct cggtgtacgc cgaggtcgaa ccgacgaagg tcaccgtctt
     4681 cgtgcgtgat cgcgggcgtg ggttcgacgt tgccgaagtg ccggaggacc ggctcggggt
     4741 gaaggagtcg atcgtcggtc gcatggagcg gcacggcggt cacgcgtacg tgcgcacggc
     4801 gccgggaatg ggaacagaag tccagctcga gatgtcgtcg ttgatggcgc gcgcatgagt
     4861 tcgcccgtga agattcgagt cgtcatcgtc gatgaccacc ggctgttccg cgccggggtg
     4921 cgggccgagc ttggtgacga catcgacgtg gtcggcgaag ccgaagacgt ccccagcgcg
     4981 gtgtcggtca tcgcgcaggc agtgcccgac gtcgtgctgc tcgatgtgca cctgcccgga
     5041 ggaggcggcg tcgaagttct gcggcagatc cggcccgtac tacccgacgt gcgctttctt
     5101 gctgtttctg tttctgacgc agcagaagat gtcatcggca cgatccgggc gggggcttgt
     5161 ggctacgtca cgaagaccat ttctggtggc gatctggccg acgcggtgac gcgcgtcgca
     5221 gagggcgacg tcgtcttctc gccgcggcta gctggcttcg tgctcgatgc atttgcagcc
     5281 accgacatgc cggcgatcga tcccgagctc gacgcgctca cccagcgcga gcgcgaggtg
     5341 ctgcggctca tcgcgcgcgg atacgcctac aaggagatcg caaaggagct gttcatctcg
     5401 gtaaagaccg tcgagaccca cgtgtcgagc gtgctgcgca agcttcaact atccaaccga
     5461 cacgagctca cgcgctgggc taccgaccgt cgtctcgtct gagcgctcga tcatgcacgc
     5521 cgtagccgac ctcgctgcgt acctgctcgg ccgctggagt atcgagcgcg agatcgtcga
     5581 tgcgcaccac accggcttct ttcgcggtaa cgccgagttc accggcgatg acggcgtcgt
     5641 cgattggatc gaggccggtc agttgcagtg ggccggtgaa acgcatcagg ccggacggcg
     5701 gttgctcctc gccgtccggc ccgattcgct ttctgtcgct gacgtcgctt tcgacgacgg
     5761 tcgcttcttc catcggctcg atctgtcccg cggctgtgac acgttcgtcc acggctgcgc
     5821 gcccgacacc tacatcggat cgtgggcagt cgatgactcc gatcagttca cgattcgctg
     5881 gaccatcgaa ggcccg
//
LOCUS       DASWVF010000452         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_88204, whole genome shotgun sequence.
ACCESSION   DASWVF010000452 DASWVF010000000
VERSION     DASWVF010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88204"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VGD55_08130"
     CDS             <1..1234
                     /locus_tag="VGD55_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0870352.1"
                     /translation="RLGSVVLNPGGPGASGIDYAEVEAPTISATLGHAFDVVSFDPRG
                     VGQSAPVKCLTAKQLDQWVAFEGNPGDPAPVNEQDQLAKNFAAGCVAKSGTLLDHVSS
                     VDTARDLDVLRAALGDAKLTYIGASYGTFLGAMYAQLFPTNIRALVLDGALDPAASAS
                     DLDHVQAQGFELALKSYIATCVKSSSCPLGTSTAGAETKLDSWLAGLQTKPIAGSGGR
                     VLTAALAVGGTAAALYSPNSWGELSSALHDAFAGRPDGLLGLSDLIDGRQSDGSYNNL
                     IESNTAINCVDRPGLGPNMAAYAREAAAGATDGPTFGEFIDWGNVACADWPVAPELAP
                     GPINPIGAPPILVIGTLRDPATPYQWAQALAKEVNGPLLTYNGDGHTAFLRSSCVDAV
                     VKAYLATLTTPAVGTTCN"
     gene            1235..1771
                     /locus_tag="VGD55_08135"
     CDS             1235..1771
                     /locus_tag="VGD55_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870353.1"
                     /translation="MEPDTAFGGVTDVRAADPRELYAACYDRLVRVLTLVAGDRAEAE
                     DVVQEAFVRLVPRWSKISAYDDPEAWVRAVAFRILSNRFRRARNAVVAARRSADIRRG
                     VDHVAETSSDGVDIARALALLPVGQRQVVVMHHLLDLPVEEIARALGVPVGTVKSRLS
                     RARVALAPLLHEGSPDHV"
     gene            1764..>2593
                     /locus_tag="VGD55_08140"
     CDS             1764..>2593
                     /locus_tag="VGD55_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870354.1"
                     /translation="MSELRDLLANEAGRGPSVSGPPFETIVARSRRRRVIQVLTVAAV
                     VIVVTAAVVVPSVVGRHDRTATVSADGGADHGATTEAARKAITQQVGEQLVAGVQLPA
                     GAVASSTAPTQALTQPFSSPGSLNLIDVVRFYTVPGTIDAVLAYAQAHPPAGTYFNGT
                     SSSADDSGVTSKGITFGHIATHDYASPTLQVVAVKFGTGVAVRLDAQLVWRPLRTLAE
                     LAPIDATGARACYGGLDCPLSLLNRADARELAAYFNSLDTQIPGDRHGCNQTTLPATF
                     T"
BASE COUNT          426 a          831 c          878 g          458 t
ORIGIN      
        1 gagactcggg tcagtcgtgc tgaatcccgg cggcccgggt gcgtcaggca tcgactacgc
       61 cgaggtcgaa gcgcccacaa ttagcgcgac actcggccac gcgttcgatg tggtgtcgtt
      121 cgatccgcgc ggagtcggcc agagcgcgcc ggtgaaatgc cttactgcga aacagctcga
      181 tcagtgggtc gcgttcgagg gcaacccggg cgacccggcg ccggtcaatg aacaggacca
      241 gctcgcgaag aactttgctg ccggctgcgt cgccaagtcg ggtaccctgc tcgatcacgt
      301 gagctcggtc gatactgcga gagacctcga cgtcctaaga gccgcactcg gcgacgcgaa
      361 actcacctac atcggtgcgt cgtacggcac cttcctcggt gcgatgtacg cgcagctttt
      421 ccctacgaac attagggcgc tggtccttga cggcgcgctc gaccccgcgg cgtccgcttc
      481 agatctggat cacgttcagg cccaagggtt cgagcttgcg ttgaagtcgt acatcgcgac
      541 gtgcgtgaaa tcgagcagct gcccactcgg gacctcgaca gcgggcgccg agaccaagct
      601 cgacagttgg ctcgcgggcc tgcaaacaaa gccgatcgca ggatcgggcg gacgcgtgtt
      661 gacagcggca ctcgccgtcg gtggaactgc cgccgcgctg tatagcccga acagttgggg
      721 cgagctgtcg tcggcattgc atgatgcgtt cgccggtcga cctgacggcc tgctcgggct
      781 gtccgacctg atcgacggac ggcaaagtga tggcagctac aacaacctga tcgagtcgaa
      841 caccgccatc aactgcgtcg accggccagg tctcggtccg aacatggctg cgtacgcgcg
      901 ggaggctgcc gcgggcgcga ccgatgggcc gacgttcggg gagttcatcg actggggcaa
      961 cgtcgcatgc gcagactggc cggtcgctcc cgaactcgcg cccggaccca tcaaccccat
     1021 cggcgcgccg ccgattctcg tcatcggtac gctgcgcgat ccagcgacgc cgtaccagtg
     1081 ggcccaggcg ctcgcgaaag aggtcaacgg tccgctgctg acctacaacg gtgacggtca
     1141 caccgcgttt ctgcgttcga gttgcgtgga cgccgtggtg aaggcgtacc tcgcaacgct
     1201 cacgacgccg gcagtcggga caacctgcaa ttgaatggaa cctgacactg ccttcggggg
     1261 tgttaccgac gtgagagccg ccgacccgcg cgagctctat gcggcgtgct acgaccgcct
     1321 ggtcagggtg ctcacgctcg ttgccggtga ccgggcagag gctgaggacg tcgtgcagga
     1381 agcgttcgtg cggctcgtgc cgaggtggtc gaagatctcg gcgtacgacg atcctgaggc
     1441 gtgggtgcgg gcggtcgcct tccggatcct gtcgaacagg ttcaggcgag ctcgcaacgc
     1501 ggtggtggcc gctcgtcgca gtgctgatat tcggcggggt gtcgatcacg tggcggagac
     1561 ctccagcgac ggcgtcgata tcgcccgagc gctcgcgctg ctgccggtcg gtcaacggca
     1621 ggtcgttgtg atgcatcacc tgctcgacct gccggtcgaa gagatcgcgc gcgcgctcgg
     1681 cgtccctgtt ggcacggtga agtcgcgact gtcgcgcgct cgggtggccc ttgctccgct
     1741 gctacacgag gggagtcctg accatgtctg aactgcgcga tttgctagcg aacgaggcgg
     1801 gccgcgggcc gtcggtgtcg ggcccgccgt tcgagacaat cgttgctcgt tctcgacgtc
     1861 gccgggtgat tcaggtgctt accgtggctg ccgtcgtgat tgtggtgacg gctgccgtcg
     1921 tcgtcccgtc tgtcgtcggt cgtcatgacc ggacggcaac ggtgtcggcg gatggtggtg
     1981 ccgatcacgg cgcgacgacg gaagcggcgc gcaaggcgat cacgcaacag gtgggcgagc
     2041 aactggtcgc gggcgtgcag ttgccggctg gcgcggtcgc gtcgtcgaca gcaccaaccc
     2101 aggcgctcac gcaaccgttc agctcgccgg gctcgctgaa cctgatcgac gtcgtgcggt
     2161 tctatacagt gcccggaacc atcgacgcgg tgcttgcgta tgcacaggca catccgcccg
     2221 ccggcacata tttcaacggg acgtcgagca gtgctgacga cagcggcgtc acgtcgaaag
     2281 gcatcacgtt tggccatatc gcgacccacg actatgcgtc cccgaccctg caagtcgtgg
     2341 ccgtgaagtt cgggacaggt gttgcggtgc ggctcgacgc gcaactggtg tggcggccgc
     2401 tgcgcacgtt ggccgagttg gcgccgattg atgccaccgg cgcgcgagcc tgctacggcg
     2461 gccttgattg cccgctgtcg ctcctgaacc gggccgacgc tcgcgagctc gctgcgtact
     2521 tcaactcact cgacacccag ataccgggcg atcgacacgg ctgcaatcag acgaccctgc
     2581 ctgcgacgtt cac
//
LOCUS       DASWVF010000453         6572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_266877, whole genome shotgun sequence.
ACCESSION   DASWVF010000453 DASWVF010000000
VERSION     DASWVF010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266877"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1185
                     /locus_tag="VGD55_08145"
     CDS             160..1185
                     /locus_tag="VGD55_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0870355.1"
                     /translation="MSFLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTNTGGIL
                     AVGLEAKRMIGRTPGNIVAIRPLKDGVIADFDTTERMLRYFIQKVHRRRYLARGPRVV
                     VCVPSGITGVEQRAVKDAGYAAGARRVYIIEEPMAAAIGAGLPVHEPTGNMVVDIGGG
                     TTEVAVISLGGIVTSQSIRIGGDELDSSIINYVKKEYSLMLGERTAEEIKMAIGSAFP
                     APDEPHAEIRGRDLVSGLPKTIVVSAAEIRKAIDEPVNAIVDAVKTTLDKCPPELAGD
                     IMDRGIVLTGGGALLKGLDERLRHETGMPIHITERPLDSVAMGSGKCIEEFEALQQVL
                     ISEPRGR"
     gene            1251..2342
                     /gene="mreC"
                     /locus_tag="VGD55_08150"
     CDS             1251..2342
                     /gene="mreC"
                     /locus_tag="VGD55_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0870356.1"
                     /translation="MRDSRRTRLVLALLILTAFTLITLDYRSRGGGFFGSVRRTASSV
                     FGPIERGAADVVRPVRHAVQAIGSIGSNHKKVTDLQRQVSALQEQLRIQPYDTARVAE
                     LEQLLHVSAVGQYKLLYAQVIAVSATDGFEWTATIDAGTRDGLKENMTVLNGDGLVGR
                     LKIVGTSTSTVLLAIDPEFNVGVRLPNNAMGVVSGHGRAAMSLMMLDPTARVKVGDGL
                     VTQGSLDQTPFVWGVPVGSVTSATDPLAGDVQTGEVQPYVDFRALDLVGVVVEPPRVD
                     PRQALLAPPLPTVTATATVTATATVTASPIFSFTPTFSPTTSPTGKPSTSRTPKPSVT
                     PTPSHSSTPTPTPTPTPRVSPSPTGSPSG"
     gene            2335..2850
                     /gene="mreD"
                     /locus_tag="VGD55_08155"
     CDS             2335..2850
                     /gene="mreD"
                     /locus_tag="VGD55_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0870357.1"
                     /translation="MAEIPRGVLIVVLVLIAGVIDAVLFDPMNLPGSPPNLLLLVVAA
                     IALVLGPVGGAITGFLAGLAADVLPPADHLIGRYALVLCLAGYLVGLLRDEARDSVFL
                     ALAAVAGAAAVATVLYGALGGVLSDGRVDAHAILRGVPFSIVYDVLLAPFVVPWVMSL
                     ARRAAPQVTRK"
     gene            2885..5290
                     /locus_tag="VGD55_08160"
     CDS             2885..5290
                     /locus_tag="VGD55_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0870358.1"
                     /translation="MNDRSGLRLLIIGALVFSLPITLLGRLWYMQALAAPQYIAAVNA
                     NATSVIHTLAPRGIIVDDVGNPLARNDSALVVSALSTALPKANLGTLKDPKPNPARIA
                     EMQRLSAVLGVPADKLDAEATLCDYFDYGKSAPDKNPGCWSGSPLQPIPLVTINNSTP
                     AGAQKATDVALRVLEQQELYPGVTAQIEDVRAYPAPDNASAAQIIGHVGKISQSDIDS
                     AKNNPTDAATLKIAAGVGGIIGQEGLEAEYNDYLEGTMGTQTFSVTPAGNPVSTLSQT
                     PATPGDTVVTSIDAKVQRIAEQALADAVNTARTKPQHISGKNQTQKADAAAAVVIDTR
                     TGHVIAAANYPTYDPGVWDGSGVDKNVYAQLQTDPGNPLFSQAIQGQYAPGSTFKVVT
                     SAAALDHGGYTLTGTYDCPGTLTEGGRTFHNFDNEGAQGMITFAQALTISCDTFFDEV
                     GATLWNADGQLAGANAQELVVNEAKLWKLGQDTGIDLPIDAKGNIESRQDLKDYDAKN
                     HALWCAEARTDKDPNQKAIDQSNCQYHIPALYQIGEAVNVAIGQGTTTASPLQMADLY
                     AAIGNGGTLYSPRLGKAIVGPDGKLVKTIDAPSTKLQVSQDVLQYIQQALVGVVTDPN
                     GTGASAFAGFPFDRYTVAGKTGTAEVPPKDDTAWFASYGGPVGQPAQYAVVVVVSQGG
                     QGGITAAPAVRQIYDGIWGLDGNPKNPHDQFAAAGPALPDGAPPVALPKLAQTAGSPA
                     AASSAPPPGSSAPSGPSASSGSAAASGSAAPAGFSLPPARLDALPVDAAPAQLRRRVA
                     KRR"
     gene            5287..6486
                     /locus_tag="VGD55_08165"
     CDS             5287..6486
                     /locus_tag="VGD55_08165"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0870359.1"
                     /translation="MMGDWAVPRPRRMSASNAGLFSRDSRLRRMDWVLTAAVLSLSLI
                     GVLLVAAATKPLNPTHPFAVVEHQVLFLAVGLALGGLAATADYRAIRSSAPVIYVLAC
                     LGLLATFAIGSSVAGAKAWIGIGGGLEIQPSEFAKLAVIVLVAMLFNTARADRRDIGD
                     LDVVRALVLMGFPMLLVLLQNDLGTMLVMVAIVFGVLAIGGVPTRWMIGLTVLGFVAT
                     VVIVQLHMLSPYQENRLTAYLHPNSPSVLKDAGYNAHQAQIAIGAGGLKGAGLFHGSQ
                     INSGAVYAAHTDFIFATAGEELGFIGSALIIVLLSVILWRGLRIASHAPDLFGRVVAG
                     GVVCWLAFETFENIGMNLGIMPVTGIPLQFVSYGGSSMFASMLAIGLLQNVHIQTQAA
                     QNSSGGL"
BASE COUNT         1112 a         2186 c         2123 g         1151 t
ORIGIN      
        1 gggaacggac aaatcgggcg ggacgccggg gagcgggcgt cgtccgcagg tttggccgag
       61 tcggtgggtc accgcgtagc atggcgaggc tgtctcggcc gggccgaaac agttcgtgtc
      121 agtcgatcgg aaggttcgca gcaacccatg gcctccaaca tgtcgttcct cggccgtgac
      181 atggccgtcg acctcggaac cgccaacacc ttggtctacg tgcgcgggcg cgggatcgtt
      241 ctcaacgagc cgtctgttgt cgcgatcaac accaacaccg gtggcatcct cgcggtcggc
      301 ctcgaggcga agcggatgat cggccgcacg cccggcaaca tcgtcgcgat ccgcccgctg
      361 aaggacggcg tcatcgccga cttcgacacg accgagcgca tgctgcgcta cttcatccag
      421 aaggtgcacc gccgcaggta ccttgctcgc ggtccgcgcg ttgtcgtgtg cgtgcccagc
      481 ggcatcaccg gtgtcgagca gcgtgcagtg aaggacgccg gctacgccgc aggtgcgcgc
      541 cgcgtctaca tcatcgaaga gcccatggct gcggcgatcg gtgccggtct gccggtccac
      601 gagcccaccg gcaacatggt cgtcgacatc ggcggtggca ccaccgaggt tgcggttatc
      661 tcactcggcg gcatcgtcac gagccagtcg attcgcatcg gcggcgacga actcgacagc
      721 tcgatcatca actacgtcaa gaaggaatac tccttgatgc tcggcgagcg caccgccgaa
      781 gaaatcaaga tggccatcgg ctccgcgttc cccgcgcccg acgagccgca cgccgagatt
      841 cgcggccgcg acctcgttag cggtctcccg aagacgatcg tcgtgagtgc tgctgagatt
      901 cgcaaggcga tcgacgagcc cgtcaacgcg atcgtcgacg cagtaaagac gacactcgac
      961 aagtgcccgc ccgaacttgc gggcgacatc atggaccgtg gcatcgtgct caccggtggt
     1021 ggcgcgctgc tgaagggtct cgacgagcgg ttgcgtcacg agaccggcat gccgatccac
     1081 atcaccgagc gcccgctcga ttcggttgcg atgggttcgg gcaagtgcat cgaggagttc
     1141 gaggcgcttc agcaggtgct catctccgag ccgcgcggtc gctgacgcgc cgacccggtc
     1201 ccggtgtcgc gcatcccctt tgatgttgcc gtgaattgcc gcgtgcgctc gtgagagata
     1261 gtcggcgcac ccgtctcgtg cttgcgctgt tgatcctgac ggcctttacc ttgatcaccc
     1321 tcgactaccg ctcgcgcggg ggagggttct tcgggtcggt gcggcgtacc gcttcgtcgg
     1381 tcttcggacc gatcgaacgc ggcgccgcag atgtcgtgcg accggtacgc cacgccgtcc
     1441 aggccattgg ttcgatcggt agcaatcaca agaaggtgac cgaccttcag cgccaggtgt
     1501 cggctctgca agagcagttg cgcattcagc cgtacgacac cgcgcgggtc gccgagctcg
     1561 aacagttgct gcacgtgtcg gctgtcggcc aatacaaact gctctacgca caggtgatcg
     1621 cggtcagtgc aaccgacggt ttcgaatgga cggcgaccat tgacgccggc acgcgggatg
     1681 gcctcaaaga gaacatgacc gtcctcaacg gcgacggtct cgtcggacgc ttgaagatcg
     1741 tcgggacgtc gacctccacc gttttgctcg cgatcgaccc ggagttcaac gtcggcgtcc
     1801 gactgcccaa caacgcgatg ggcgtcgtgt cggggcacgg ccgcgcggcg atgagcctca
     1861 tgatgctcga cccgacggcc cgcgtgaagg tgggcgacgg cctcgtgacg cagggctcgc
     1921 tcgaccagac ccccttcgtg tggggggtcc cggtcggcag cgtcacatcg gccaccgacc
     1981 ctctcgccgg cgacgtgcaa actggagagg tgcagccgta cgtcgatttc agggccctcg
     2041 atttggtggg cgtcgtggtc gagccgccgc gagtcgaccc ccggcaggcc ctgctcgctc
     2101 cgccactgcc gacggtcacg gcaacggcga cagtgactgc gaccgcgacg gtgacggcaa
     2161 gcccgatctt ctcgttcacg cccactttca gccccacgac gagcccgacc ggcaagcctt
     2221 caacaagccg gacgccgaag ccgagcgtca caccgacgcc gagccacagc tcgacgccga
     2281 cgccaacgcc gacgccaacg ccaagggtct caccgagccc gaccgggagc cccagtggct
     2341 gagattccac ggggcgtgct gatcgtcgtc cttgttctga ttgccggtgt catcgacgcc
     2401 gtgctgtttg acccgatgaa cctgccgggg tcgccaccga acctgctact gctcgtcgtc
     2461 gccgcaatcg cgctcgtgtt gggtccggtc ggaggtgcca tcaccggttt tctcgcgggc
     2521 ctcgcggccg acgtcctgcc gcccgccgac cacttgatcg gccgctacgc gctcgtgctg
     2581 tgcctggccg gttacctcgt cggactgctg cgcgacgagg cacgcgactc ggttttcctt
     2641 gctcttgcag cagttgccgg tgcagctgcg gtcgcgaccg tgctgtacgg cgctctcggg
     2701 ggagtgctta gtgacgggcg cgtcgacgcg cacgcaatcc tgcgtggggt gccgttttcc
     2761 atcgtctacg acgtattgct cgcgccgttc gttgtgccgt gggtgatgtc gctcgctcgt
     2821 cgcgccgcac cccaggtgac ccgcaagtga acgcgccgtt cgtcgccgcg ggaaaggcgt
     2881 acttatgaac gatcgatcag ggctgcgcct actgatcatc ggcgccttgg tgttctcgct
     2941 gccgatcaca ctgctcggtc gcctctggta catgcaggca ctggccgcgc cgcaatacat
     3001 cgctgctgtg aacgccaacg ccaccagcgt gatccacaca ctcgcgccgc gcggcatcat
     3061 cgtcgacgac gtcggcaacc cgctcgcacg caacgacagc gcgctcgtcg tctccgcgct
     3121 cagcacggcg ctgccgaaag ccaacctcgg caccttgaag gatcccaagc ccaacccggc
     3181 ccggattgcg gaaatgcaac ggctgtcggc agtcctcggc gtcccggctg acaagctcga
     3241 cgccgaagcg acgctgtgcg attacttcga ttacgggaag agcgcaccag acaagaaccc
     3301 cggctgttgg agtggttcgc cgttgcagcc gatcccgctg gtcaccatca acaacagcac
     3361 gcctgccggt gcgcagaagg cgactgacgt tgcgctgcga gtgctcgagc agcaggagct
     3421 ttacccggga gtgactgcgc agatcgaaga cgtccgcgcc taccccgcgc ctgacaatgc
     3481 atcggccgcg cagatcatcg gccacgtcgg caagatcagc cagtccgaca tcgacagcgc
     3541 gaagaataat cccaccgacg cagccacatt gaagatagcc gccggcgtcg gcggcatcat
     3601 cggccaagag gggctcgagg ccgagtacaa cgactacctc gaaggcacga tgggcacgca
     3661 gacgttctcg gtgacgccgg ccggcaaccc ggtttcgacg ctgagccaaa caccagcgac
     3721 gccaggcgac accgtcgtca cgagcatcga cgccaaggta cagaggatcg ccgaacaagc
     3781 gttggccgac gcggtcaaca ctgcgcgtac gaagccgcag cacatctcgg gcaagaacca
     3841 gacgcagaag gcggacgccg cggcagctgt cgtcatcgac acccgcaccg gtcatgtgat
     3901 cgctgccgcg aactacccga cgtacgaccc gggagtctgg gacggcagtg gcgtcgataa
     3961 gaacgtgtat gcccagctcc aaactgatcc tgggaacccg ttgttctcgc aagctattca
     4021 gggccagtac gcgccgggct cgacgttcaa ggtggtcacc agcgctgcgg cgctcgacca
     4081 cggcgggtac acgctgacgg gtacgtacga ctgcccggga acgttgacgg aaggtggccg
     4141 aacgttccac aacttcgaca acgagggcgc gcagggcatg atcacgtttg cccaggcgct
     4201 gacgatctcg tgtgacacgt tcttcgacga ggttggcgcg acgctgtgga atgccgacgg
     4261 gcaactcgct ggtgccaacg cgcaggaact cgtcgtcaac gaggcgaaac tctggaagct
     4321 cggccaggac accggcatcg atctgccgat cgacgcgaag ggcaacatcg agtcgcggca
     4381 ggacctgaag gactacgacg ccaagaatca cgcgctgtgg tgcgcggagg cgaggaccga
     4441 caaggatccg aaccagaagg cgatcgacca gagcaactgc cagtaccaca tccccgcgct
     4501 ctatcagatc ggcgaagcgg tcaacgtcgc gatcggtcag ggcacgacga ccgcatcgcc
     4561 gcttcagatg gccgatctgt acgcggcgat cggcaacggc ggcacccttt actcaccgcg
     4621 cctcggcaag gcgatcgtcg gtcccgacgg caagctcgtc aagacgatcg acgcgccttc
     4681 gacgaagctt caggtgtcgc aggatgtctt gcagtacatc cagcaggcac tcgtcggggt
     4741 cgttaccgat cccaacggca ccggcgcttc tgcgttcgcg ggcttcccgt tcgatcgcta
     4801 caccgtcgcc ggaaagaccg gaacagccga agtcccgccg aaggacgaca ccgcatggtt
     4861 cgcctcctac ggtggcccgg tcggccagcc cgcgcagtac gcggtcgtcg tcgtggtctc
     4921 ccaaggcggt cagggcggaa tcactgccgc acctgcggtg cggcagatct acgacggcat
     4981 ctggggtctc gacggcaatc ccaagaaccc gcacgatcag ttcgctgcgg ctggcccggc
     5041 gctgcctgac ggtgcaccgc cggttgcgct gccgaaactc gcccagactg ctggctcgcc
     5101 ggcggccgcg agttcggcgc caccgccggg gtcgtcggca ccgtcaggcc cgtcggcctc
     5161 gtcgggctcc gctgcggcgt cgggctccgc ggcgcctgcc ggtttctccc tcccgcccgc
     5221 gcggcttgac gcgctgcccg tcgatgcggc gcccgctcaa ctgagaaggc gggtggcgaa
     5281 gcgacgatga tgggtgactg ggcggtgccg cggccgcgcc gcatgagtgc ctccaatgca
     5341 gggcttttca gccgcgactc gcggttgcgc cggatggatt gggtgctgac cgcggcggtc
     5401 ttgagcctgt cgctgatcgg tgtgctgctc gtcgccgccg cgacgaagcc actgaacccg
     5461 acacatccct tcgcggtcgt cgagcatcag gtgctgttcc tcgccgtcgg gctcgcgctt
     5521 ggaggccttg cggccacggc ggattaccga gcgatcaggt cgtcggcgcc tgtcatctac
     5581 gtgctcgcgt gcctcgggct tcttgccacg ttcgccatcg gctcgagcgt cgcaggggca
     5641 aaggcgtgga tcggcatcgg cggcggtctt gagatccagc cgtcagagtt cgccaagctc
     5701 gcggtcatcg tgttggtcgc gatgctgttc aacacggcac gtgcggatcg gcgcgatatc
     5761 ggcgacctcg acgtcgttcg agccctggtg ctcatgggtt ttccgatgtt gctcgtgctg
     5821 ttgcagaacg acctcggcac gatgttggtg atggtcgcga tcgtgttcgg ggtactcgcc
     5881 atcgggggtg ttccgacccg ctggatgatc ggcctgaccg tgctcgggtt cgtggcgacg
     5941 gtggtcatcg tccagctcca catgctcagc ccttaccagg agaatcggct gaccgcgtac
     6001 ctgcatccga actcaccgtc ggtgctcaag gatgcggggt acaacgcgca ccaggcgcag
     6061 attgcgatcg gtgccggtgg cctgaagggt gccgggctct tccacgggtc gcagatcaac
     6121 agcggcgcgg tctacgccgc acatactgac ttcatcttcg cgaccgcggg ggaggagctc
     6181 ggcttcatcg gttctgcgtt gatcatcgtc ttgttgtcgg tgatcttgtg gcgtgggttg
     6241 cggatcgcgt cacacgctcc ggacctcttc ggtcgcgtcg ttgccggcgg cgtggtgtgc
     6301 tggctcgcgt tcgagacctt cgagaacatc ggcatgaacc tcggcatcat gccggtcacc
     6361 ggaatcccgc tccagttcgt ctcctatggc ggctcgtcga tgttcgcgag catgctggcc
     6421 attggtctgc tccagaacgt ccacatccag actcaggcgg cgcagaattc cagcggcggc
     6481 ctttagagct agtcttcaag cgtgtcgagc cccgtattga gccccgccct tagccagagt
     6541 tccgagtcgg tttttgcgcg gctcgaaccg tt
//
LOCUS       DASWVF010000454         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_343106, whole genome shotgun sequence.
ACCESSION   DASWVF010000454 DASWVF010000000
VERSION     DASWVF010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343106"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..865)
                     /gene="istB"
                     /locus_tag="VGD55_08170"
     CDS             complement(95..865)
                     /gene="istB"
                     /locus_tag="VGD55_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY0870360.1"
                     /translation="MSEIIRASVEERLARLRLNSVAMRLDAILSQAARKEPTYLDFLD
                     EVLGDELDAKQKKRVAMAIQIAHFPTVKLLEEFDFKFQPSVDQRLVRELATGHFIAGA
                     ENVLLFGPPGVGKTHLAIGLGRAVVEAGHSVLFITATSLIGSLAKAESEGRLAEQIGF
                     FAKPKLLIVDELGYLPFEKRSANLFFQLVTRRYQRGSMLLTTNQMVTQWDQVFGDEMI
                     AAAVLDRLLHHSHALVVQGESYRLRQKKRAGLVARSAG"
     gene            complement(862..2087)
                     /gene="istA"
                     /locus_tag="VGD55_08175"
                     /pseudo
     CDS             complement(862..2087)
                     /gene="istA"
                     /locus_tag="VGD55_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(2516..2668)
                     /locus_tag="VGD55_08180"
     CDS             complement(2516..2668)
                     /locus_tag="VGD55_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870361.1"
                     /translation="MQKGTSVVTQLVIAAVVVGALAFVSGLAVVLLVGTRVTGGLRER
                     PEESLC"
     gene            complement(2961..>3665)
                     /locus_tag="VGD55_08185"
     CDS             complement(2961..>3665)
                     /locus_tag="VGD55_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0870362.1"
                     /translation="VRGRDLASTIAEAQRRIAEQVKLPYDTHLEWAGEINQLNEATGR
                     LALIIPITLLVIAMLVYSSVKNWKDMLIVLGGIPVACCGGIMALLVTRTNFSISAAMG
                     FISIFGIAVQDALIVVTYAQRMWDEGHGLEEGARIAAERRLRPVLMTTFVAMLGLTPA
                     ALSHGIGADTQKPLAIVVIGGALILAVLPRLLQPPLLVLAHARERRWTRRSEAQRILD
                     AKRAESADAVLRPTDG"
BASE COUNT          629 a         1247 c         1193 g          596 t
ORIGIN      
        1 ggcgtgccgc tgctgctgcc taagaagctg tcaaacgaca tcgggaagtg acccccttac
       61 gacactaaaa gtgaccccct catttgttca gtgactaccc cgcgctcctg gccaccaggc
      121 cagcgcgctt cttctgcctg agtcgatagc tctcgccctg cacgacgagg gcgtggctgt
      181 ggtgcaggag tcggtcgagg actgcggcgg cgatcatctc gtcgccgaag acctggtccc
      241 actgcgtgac catctggttg gtcgtgagca acatgctgcc gcgctggtag cggcgcgtga
      301 cgagctggaa gaacaggttg gcgctgcgct tctcgaaggg caggtatccg agctcgtcga
      361 cgatgaggag cttcggcttc gcgaagaagc cgatctgctc ggcgagacgg ccttcggact
      421 cggccttggc gagcgagccg atcagcgacg tcgccgtgat gaagagaacc gagtgtccgg
      481 cctcgacgac ggcgcgtccg aggccgatcg cgaggtgagt cttgccgacg ccgggtgggc
      541 cgaagagcag gacattctcg gcgccagcga tgaagtggcc cgtcgcaagc tcgcgcacga
      601 gccgctgatc cacggacggc tggaacttga agtcgaactc ttcgagcagc ttcaccgtgg
      661 ggaaatgcgc gatctggatg gccatcgcga cgcgcttctt ctgcttggca tcgagctcgt
      721 cgccgagaac ctcgtcgagg aagtcgaggt aggtcggctc tttgcgggcc gcctgcgaca
      781 ggatcgcgtc gagcctcatc gctacggaat tgagacgcag tcgcgcgagc cgctcctcga
      841 cgctggcacg aatgatctcg ctcaagccgc acctgcgatg acggcggcgt agtcgtcgag
      901 cgtgcggccg tgcgccgcca acagcggcga caccgcggct acgtcgtcgc gtggccgcat
      961 gagaccggcg aagtggcctg gctccacgac gcgcgtaaac ggctcgcgaa cgcgacgatg
     1021 tcgcgcgacg acctcgacgc cgtcgaagat cacgacctcg tcgtcgccga gaaggacatc
     1081 gacggtacgg cgcacgagac ggtgcggcac gctgtaacgc acggtctcga cgtcgacgaa
     1141 ggagtccgtg ggaacacgcc gagacaggcg ctgctgtcgg accggcagcg gcctcgctgg
     1201 caacggctgc agggcctgcc tctctcctca cgctcgaagc gatcgagagg ccgctcgtgc
     1261 gtcgtgccgt gagtccgcgt gtcggcggcg atgatccacg tcgcgagatg ggcctccagc
     1321 gcgatgaagc tgtcgaaggt gcggccggcg atggcattgc gcttcacgta gccaacgccg
     1381 gactccgtct tgccctttgt ccgtgctcgg tacgggcggc aggctcgcgg cgcgacgccc
     1441 cagtctgcgc agaacgcgct gaatgcaggg tgcacatgca cgacgcccgc ctcggtgtcg
     1501 cgcccgagaa tcagcgcgcg cgcattgtcg acgagcaccg tctgcgtgac gccgccgaag
     1561 tggaggaacg caccggcgag tccctcgcgc cagtcgtcgt gtcgctcgcg tagaaatgct
     1621 cgcacgtaga tccgacgcga gtagccgagc acggcgacga agaagtagac ccgcatctct
     1681 cggccggcga tgagcacgcg tcgctcgccg aagtcgatct ggagctggtg gcctggcgca
     1741 gtctcgaagc gaatcgtcgc cacctcggcg gcgcgacggg cctgccggtg cggcgcaacc
     1801 gcccgctgca gcgtgcgcag ctccacctcg atccctcgct cgccgagaag ctgccgcgcg
     1861 acgaccgcat tcccggccgc ctcaccgtcg aggagtgcca cggccaccgt gcgctgagcg
     1921 gcgtcgagcc gtcgcgctcg aggcctctcc tgcgctcccg gcgtcgcacc acgcaggtac
     1981 cgccgagcgg caacgcggct gatcccgagc gtcgcggcga tacgcttgga gccccagctc
     2041 atcgcggcca accctcgaat ctgccgcacg atctcaggct ccaacatcgg cacctccgac
     2101 ggtcggagtg ccacgcctgt ctgtctctgg tctatcggtc cctcctgtcg ggagggggtc
     2161 gcattttagt gtcgtttggg ggtcagttta cgtgtcgcct gacacccggc gcctggcagg
     2221 gaggtcgtcg cagcgctctc gccgctcgcc tcccaccgtg ccaccaactt tggccaatct
     2281 gtgcgcttgc accgtatgcc ccgacaacta cgccacgccg ccctttatgt cagctacccc
     2341 agtcgtcgaa cggatacaca cattccgtcc ccgggagagg catctgtgtc aggcacacaa
     2401 aaggtgcgaa cggcctcggc accgtcgcag atcgccgagc ggccgggact cagcccggac
     2461 tgctcggccc cgtgtcattc cgaatcgaca ctggccgggc gcggacccct gacggtcagc
     2521 agagactctc ttccgggcgc tctcgcaatc cgcccgtcac tctggtcccg acaagcagga
     2581 cgaccgcaag accgctgaca aaggccagtg ctccgacgac gacggcggcg atgacgagct
     2641 gggttacgac ggacgtgccc ttttgcaaag ctgctcctgc ggcttgagcg cactcagagt
     2701 tccgtcctgc gatcgcggcc gcgcagtctc gcgggtcgtc ggggtccgaa caagtcgagg
     2761 tacgctcagc ggtcgaccag aagcggtagc gaccgccgag cggatggagc cgagactcat
     2821 tgtcattgac acgcctaata cgggctgcgg gcggcggaac tgcgggcgaa gtccgactgg
     2881 cctgcctgaa attctgtcgg cgccgaaatc gggtgcgaag agcgtgatca ctgccgcccc
     2941 gccgcccgcc cccacgacgg ctatccatca gtcggcctca ggacggcatc agcggactcg
     3001 gcccgcttcg cgtcgagtat ccgctgcgcc tccgaacgcc tggtccatcg gcgctcgcgc
     3061 gcgtgcgcga gcaccagcaa cggcggctga aggagccgcg gaagaacggc caagatcagc
     3121 gctccgccga tcacgacgat cgcgagcggc ttttgggtat ctgcgccgat gccgtgcgag
     3181 agcgccgccg gggttagccc gagcatcgcc acgaacgtgg tcatcaacac gggccggagg
     3241 cggcgctcgg cggcgatgcg ggcgccctcc tccaggccgt ggccttcgtc ccacatccgc
     3301 tgggcgtatg tcaccacgat gagagcgtct tgcaccgcga ttccgaagat cgaaatgaac
     3361 cccatcgcgg cggagatgga gaagttcgtc cgcgtcacca gcaaagccat gatcccgccg
     3421 caacacgcga ccgggatacc gcccagcacg atcagcatgt ctttccagtt cttgaccgac
     3481 gaatagacca gcatagcgat caccagcagc gtgatcggga tgatcagcgc caggcgtccc
     3541 gtggcctcat ttagctgatt gatctccccc gcccattcga ggtgcgtgtc gtagggcaac
     3601 ttcacctgct cggcgatcct ccgttgcgct tcagcaatgg tcgacgccag atcccggccg
     3661 cgcac
//
LOCUS       DASWVF010000455         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_190339, whole genome shotgun sequence.
ACCESSION   DASWVF010000455 DASWVF010000000
VERSION     DASWVF010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190339"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..>1545
                     /gene="treZ"
                     /locus_tag="VGD55_08190"
     CDS             72..>1545
                     /gene="treZ"
                     /locus_tag="VGD55_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY0870363.1"
                     /translation="MRELRVWAPLAQKVAVQITHPTTARTWTRSLVEVGEGWFAADVD
                     EAKDGWTYGFLLDDDGQLLPDPRSRRQPTGVHGPSAFYDDTFEWTDGSWEGRALPGTV
                     FYELHIGTFTDEGTFDAAIKRLDYLADLGVTHLELLPINDFNGLWNWGYDGVLWYATH
                     EAYGGPDGLKRFVNACHRRGLAVTLDVVYNHLGASGNVLPRFGPYLKSGRNTWGDLLN
                     LDQENSRPVRDYIIDNACMWLSEFHVDALRLDAVHALRDDSSLHLLAELSQRVEELAG
                     TVARPLTLVAESDLNDPVMVTPRALGGYGLDAQWDDDVHHCLHALLSGERHGYYADFG
                     SLAAVAKVMSRAFFHDGTYSSFRGRTHGKLVDREAIPGYRFVVCLQNHDQVGNRAAGD
                     RLTDIVDPDLLKVGAVLLLTSPFTPLLWMGEEWGATTRWPYFTSHPEPELAEAVHHGR
                     IEEFRGHGWDLSTVPNPQDLATFHSAKLDWAEREAPGHADM"
BASE COUNT          281 a          520 c          490 g          254 t
ORIGIN      
        1 cgccggcggc gttgtccgcc tagacaccgt cctatccgaa ctccccgtcg cgctgctgct
       61 aaggagcgag cgtgcgtgag cttcgagtct gggctcccct ggctcagaag gtagcggtgc
      121 agatcaccca ccccaccacg gcgcgcacct ggacccggtc gctggtggaa gtgggtgagg
      181 gatggttcgc tgcggacgtc gacgaagcaa aggacggctg gacctacggc ttccttctcg
      241 acgacgacgg gcagctgctg cccgatccgc gctcacgacg ccagccgacc ggcgtccacg
      301 ggccgagcgc cttttacgac gacaccttcg agtggaccga cggctcatgg gaaggccgcg
      361 ccctaccggg aaccgtcttc tacgaactgc acatcggaac cttcaccgac gagggcacgt
      421 tcgacgccgc catcaagcgg ctcgactacc tcgccgacct tggggtgaca cacctcgagc
      481 tgctgcccat caacgatttc aatggcctgt ggaactgggg ctacgacggg gtgttgtggt
      541 acgccacgca cgaggcctac ggggggccgg acggcctgaa acgcttcgtc aacgcctgtc
      601 atcgccgcgg tctcgcggtg actctcgacg tcgtctacaa ccaccttgga gcaagcggca
      661 acgttctgcc gcgattcggg ccttacctga agtcggggcg caacacgtgg ggagacctgc
      721 tcaacctcga ccaagagaac tcccggccgg tgcgcgacta catcatcgac aacgcctgca
      781 tgtggctctc cgaattccac gtcgacgcgt tgcggctcga cgccgttcat gcgctgcgcg
      841 acgacagctc gctgcacctg ctggcggagc tgtcgcaaag ggtcgaagag ctggcgggca
      901 cggtagcacg cccactcacg ctggtggccg agtcggatct gaacgacccg gtcatggtga
      961 ccccgcgcgc gctcggcggc tacggcctcg acgcgcaatg ggacgacgac gtacatcact
     1021 gcctgcacgc gttgctgtcc ggcgaacgcc acggctacta cgcggacttt ggctcgctcg
     1081 ccgcggtggc gaaggtgatg agccgggcgt tcttccacga cggcacctac tcgagttttc
     1141 gcggcaggac ccacgggaag ctggtggacc gggaggccat cccgggttac cggttcgtcg
     1201 tctgcttaca aaaccacgac caggtcggca accgcgcagc cggcgatcgg ctaacggaca
     1261 tagtcgaccc cgacctgctc aaggtcgggg ccgtgctgct actaacttca ccgttcacac
     1321 cgctcttatg gatgggcgaa gaatggggcg caacgaccag gtggccgtac ttcacctcac
     1381 atcccgagcc agaacttgcc gaagccgtgc accacggacg catcgaggag ttccgcggac
     1441 acggttggga tctgtcgaca gtgcccaacc cgcaggatct cgcgacgttc cattcggcca
     1501 agctggactg ggccgagagg gaagcgcccg gccatgccga catga
//
LOCUS       DASWVF010000456         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_88314, whole genome shotgun sequence.
ACCESSION   DASWVF010000456 DASWVF010000000
VERSION     DASWVF010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88314"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1051
                     /locus_tag="VGD55_08195"
     CDS             101..1051
                     /locus_tag="VGD55_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870364.1"
                     /translation="MTATDTVTTSITGTSGTITVVARTSPTRAAGSDRIGTAVTVSQL
                     DFPSGGAGAVVLARSDDFPDALVAAPLAATVRAPLLFVQGGSLPTVTRGEISRVLGAR
                     GTVYLIGSTSAIPTSVETSLQTLGYATTRIGGSDRYATAAAVANVLGNPRTVFLATGI
                     NFPDALSAGPAAAHTGGVVLLTDGSSMPSITRSYLNAHVGTVYAIGGPAAGADPAATR
                     IVGADRMSTAASVASTFFTAPLAVGVASGATFADAMSGGAYLARLGGPILLTYPTSVP
                     ASTSNYLTAVRSSLATSSIFGGTAAISLSVQTTIDHLLGF"
     gene            complement(1212..1589)
                     /locus_tag="VGD55_08200"
     CDS             complement(1212..1589)
                     /locus_tag="VGD55_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870365.1"
                     /translation="MNAGHDRRLLRRSAIASRDGVVFRVGLAALVVGLIGVIPAFALA
                     QDEQTRFGQKASHALVTVAGVVRASDAATLLVGAILLTVSSIYRSRELRAEAHATAGV
                     DPAFMAELPATPGMVPRFNPPPG"
     gene            complement(1611..>1937)
                     /locus_tag="VGD55_08205"
     CDS             complement(1611..>1937)
                     /locus_tag="VGD55_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870366.1"
                     /translation="ISLHTPLGEDGDSEFGDLIEDSEAIVPAEAVSFTLLQEQLHSVL
                     DTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQV
                     LRDYLD"
BASE COUNT          324 a          662 c          627 g          324 t
ORIGIN      
        1 cacatcgtcc gacacttcag catcgctgag tgcgaacgcc aaactgacga acggaatagg
       61 caccttcatt gccacgctgc gaacgatcgg cagccgcacg gtgacggcga cggacacggt
      121 caccacctcg atcaccggga cgtcgggcac cataacggtc gtcgcccgga cgtcgccgac
      181 ccgcgccgca ggatctgacc gcatcggcac cgcggtcacg gtgtcacagc tggacttccc
      241 cagcggtggg gcgggcgccg tcgtgctcgc ccgttccgac gatttccccg acgcgctggt
      301 tgcagcgccg ctggcagcga cggttcgggc gcccctgctg ttcgtccagg gcgggtcatt
      361 gccgaccgtg acgcggggcg agatcagccg ggtgctcgga gcgcgcggga ccgtgtatct
      421 gattggcagc acgtccgcaa tcccgacgag cgtcgagacc agccttcaga cgttgggcta
      481 cgcgaccacg cgcatcggag gctccgaccg gtacgcgacg gcagcggcgg tggccaatgt
      541 ccttggcaac ccgcgcaccg tgtttctcgc aacgggcatt aacttccctg acgcgttgtc
      601 agctgggccg gcggccgccc acactggcgg cgtcgtgcta ctcactgacg gctcgtcgat
      661 gccgtcgatc acccgctcgt atctgaacgc gcacgtcggc acggtctacg cgatcggtgg
      721 ccccgctgcg ggagcggatc cagccgcaac ccggatcgtc ggcgccgacc ggatgtcgac
      781 cgccgcatca gtggcgtcga cgttcttcac ggcgccactg gccgtcggcg tcgccagcgg
      841 agccacgttc gccgatgcga tgtcgggagg tgcctatctc gcccgactcg gtggtccaat
      901 tctgctgacc tacccgacgt cggtaccggc atcgaccagc aactacctca ccgctgttcg
      961 atccagcctg gcgacaagca gcatcttcgg aggcaccgca gcgatctcgc tcagcgtgca
     1021 gacgacgatc gatcacttgc tcggcttctg atcgatcggc ggggaccttc acgttccccg
     1081 tcggaaggca gccgtcagcc gagatccgct ggtccgtgaa cgatgagttg ccacccgacc
     1141 ggcgttgggg gccacgtcgg gtccggtgac catcccggtt cgggcagcca gtcgcgcgtg
     1201 ggcggcgtcg gtcagcccgg tggcggattg aaccggggca ccattccagg tgtcgcaggc
     1261 aactccgcca tgaaggccgg gtcgactccc gccgtcgcgt gggcttcagc gcgcagttca
     1321 cgcgagcggt agatcgatga caccgtgagc aagattgcgc cgacgagcag cgttgcggcg
     1381 tcgcttgccc tgaccacgcc agcgaccgtg acgagcgcgt gactggcctt ctgtccaaag
     1441 cgagtctgtt cgtcttgagc gagcgcgaac gcgggaatta caccgatgag gccgacgacc
     1501 aacgcggcta aaccgacccg aaacaccacg ccgtcacgcg acgcaatcgc actccgccgc
     1561 aggagacgac gatcgtggcc cgcgttcaca actcccccag tggcggagcc ttagtcgagg
     1621 tagtcgcgca gcacttgtga gcgcgatggg tggcgtagct tcgacatcgt cttcgactcg
     1681 atctgacgga ttcgctcgcg agtgactccg tagaccttgc cgatctcgtc gagcgtcttc
     1741 ggctgaccgt cagtgagacc aaaccgcatg gagacgaccc cggcctcgcg ctcggacagc
     1801 gtgtcgagca ccgagtgcag ctgttcctgc agcagtgtga atgacacggc ctcggcgggg
     1861 acgatggcct cggagtcctc gatcaggtcg ccgaactcgc tgtcgccgtc ttcaccgagc
     1921 ggcgtgtgca aactgat
//
LOCUS       DASWVF010000457         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_445335, whole genome shotgun sequence.
ACCESSION   DASWVF010000457 DASWVF010000000
VERSION     DASWVF010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445335"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VGD55_08210"
     CDS             complement(<1..2201)
                     /locus_tag="VGD55_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0870367.1"
                     /translation="MQSDRSNDPVSERRTASLLFADMVGFTPIAEARDPEAVRDLLST
                     YFDRARAIIERYNGTVEKFIGDAVFAVWGVPAAREDDAERAVRAGLDLVATARVLGEE
                     FGIEALQMRVGVSTGQVAVTLGAVAADGQGVVAGDAVNTAARIQAAAAPGEVWVDDTT
                     RSLTTASLAYVSTGSHELKGKSFPVELYHAMRTTAAAGGAQRVDGLEAPFVGRDRELR
                     LVKELFHATAEERRARLVLVAGEPGIGKSRLAWEFEKYADAITQRTHWLRGRCLSYGQ
                     GVAGRVVAEMVRSLLRVTETDDDATVTAALDERLARHVTDEVERDVLRPRLASLLGLS
                     DAVFDQADLFSCWRAFFESLCVADESVTMIVEDLQWADDGFLDFLDYLLDVSRAAIMV
                     LALARPEVTTRRAGIGAGRRSSTVFLEPLPDDVMVRLLDGLVGDLPRELRTELVARAE
                     GVPLYAVETIRALIDRDVVVASGGRYVVDPEAAGALDLSALGPPASLQALLAARLDAL
                     PAAERRVVQDASVLGLSFSRAGIGALAPSDIELDAVLESLRRKEILIVDNDPRSPERG
                     QYRFVQALLRTSAYDTLSRRDKHSRHLAAVEHLAALPDADTIAGVLAAHLLDAISAMP
                     DALDVAILRNRAAQLLEQAAVHAAGVGAPTDALAHYGQLFALDLPDESVARLAIAAST
                     IALDAAARLDESDQWVTAGMLAAQRCGDEENLLRLEMHRSRMLAVRGDMAEA"
BASE COUNT          433 a          837 c          790 g          367 t
ORIGIN      
        1 gcctccgcca tgtcgccgcg caccgcaagc attcgtgagc gatgcatctc gagacgcagc
       61 aggttctctt cgtcaccgca gcgctgcgcc gcaagcatgc cggcagtcac ccattgatcg
      121 ctctcgtcga gccgcgcggc cgcatcgagc gcgatagtcg acgcggcgat cgcgagacgg
      181 gcaacggatt catctgggag gtcgagcgcg aacagttggc cgtagtgcgc aagcgcatcg
      241 gtcggcgcgc cgacccctgc ggcatggacg gcggcttgct ccagcagctg cgctgcacgg
      301 ttgcgcagga tcgcgacgtc gagcgcgtcg ggcatcgccg aaattgcgtc aagcaagtgg
      361 gcggcgagca cgcccgcgat cgtgtcggca tcaggaaggg cggcgagatg ctcgaccgct
      421 gccagatgtc gcgaatgctt gtcacgcctc gacaacgtgt cataggcgct cgttcgaagc
      481 agtgcctgaa cgaaccggta ctgcccacgt tcgggcgagc gagggtcgtt atcgacgatc
      541 agaatctctt ttcgccgcag gctttccagc accgcatcga gttcgatatc ggacggcgca
      601 agagcgccga tgccggcgcg actgaaactc aagccgagca cgctcgcgtc ttggaccacc
      661 cgacgctcgg cggccggcaa cgcatcgagg cgcgcggcga gtagcgcctg caggctcgca
      721 gggggcccga gagccgacaa gtcgagtgcg ccggctgcct cggggtcgac gacgtatcgg
      781 ccacctgaag cgacgactac gtcgcggtcg atcagcgccc gaatcgtctc gacggcgtag
      841 agcggtacgc cctcagcgcg ggcgacgagc tcggtgcgta gttcgcgagg caggtcgccg
      901 accaggccgt cgagtagccg gaccatcacg tcgtcgggca acggctcgag gaataccgtg
      961 ctcgagcgcc ggccggcacc gatgcccgcg cgacgggtcg taacctcggg acgggcaagg
     1021 gccagcacca tgatcgccgc ccggctgacg tcaagcagat agtcgaggaa gtcgagaaat
     1081 ccgtcgtcgg cccactgcag gtcttcgacg atcatggtga ccgactcgtc ggcgacacag
     1141 agcgactcga agaaagcccg ccaacacgag aacaggtcgg cctggtcgaa cacagcgtcc
     1201 gacagaccga gcaagctagc gaggcgtggc cgcagcacgt ctctctcgac ctcgtcggtg
     1261 acgtgccggg caaggcgttc gtcgagtgcc gccgtcacag tcgcgtcgtc gtccgtttca
     1321 gtgacccgca acagcgaccg caccatttct gcgacgacgc ggcccgcaac cccctggccg
     1381 taagacaggc agcgaccccg caaccaatgc gtgcgctgcg tgatcgcgtc tgcgtatttc
     1441 tcgaactccc acgccagccg cgacttcccg atgccgggtt cgccggcgac caacacgagt
     1501 cgcgctcggc gctcctccgc ggtcgcgtgg aacagttcct tgacgagccg caactcacga
     1561 tcgcggccga cgaatggcgc ttcgagtccg tcgacgcgtt gcgcaccgcc agccgccgcc
     1621 gtggtgcgca ttgcgtggta gagctcaacc gggaacgact tgcccttcag ctcatggctg
     1681 ccggtcgaga cgtaggcaag cgaagcggtc gtcagcgagc gggtcgtgtc gtcgacccag
     1741 acctcaccag gggcagcagc tgcctggata cgagccgctg tgttgaccgc atcacccgcg
     1801 accacaccct gcccgtcagc ggcgaccgcg ccgagggtga cggcaacttg tccggtgctc
     1861 accccgactc gcatctgcaa cgcctcgatg ccgaactcct cgccgagcac ccgcgcggtc
     1921 gcgaccaggt cgagcccggc tcgcaccgcg cgctcggcat catcttcgcg cgccgcagga
     1981 acgccccaga ccgcgaacac tgcgtcgccg atgaacttct ctacggtgcc gttgtagcgc
     2041 tcgatgatcg ctcgcgcacg gtcgaaatag gtcgagagca gatcacgaac cgcctcggga
     2101 tcgcgtgcct cggcgatggg agtgaagccc accatgtcgg cgaacagcag cgacgcggtc
     2161 cgtcgctcag agaccggatc gtttgagcgg tcactttgca cggcttgccc gggtacggag
     2221 ggtgcgctgt cacgcccagg aatcggcgtc ccgcattcac tgcagaaccg accaccagtg
     2281 agcggtgctc cgcacgcggc acacaactgc gacatggctt ggccgcattc gctgcagaac
     2341 ttcgcgccag cacggtttac cgccccgcat cggctgcatg cggtgtccga cccggcgccg
     2401 ggcacgaggg cctccttcga ggtacac
//
LOCUS       DASWVF010000458         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_12157, whole genome shotgun sequence.
ACCESSION   DASWVF010000458 DASWVF010000000
VERSION     DASWVF010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12157"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGD55_08215"
     CDS             complement(<1..452)
                     /locus_tag="VGD55_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870368.1"
                     /translation="MFAQEGVPRDPWEKIADAAQVHRFTGVAPTVALHIPWDAVDDYS
                     KLSRHANDLGVAIGTINSNVFQDNDYMLGSVTNPSERVRRKATDHLLACIDIMDATGS
                     RDLKLWFSDGTNYPGQDDIVTRQRRLADALGKVYARLGADQRMLLEYK"
     gene            complement(578..>1516)
                     /locus_tag="VGD55_08220"
     CDS             complement(578..>1516)
                     /locus_tag="VGD55_08220"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0870369.1"
                     /translation="PPARPDDARTDRYLVATAYHARTAQVLSDVAGVLGHNDDQRRYA
                     TRAAKVRVAFADEYVAPSGRISSDSQTAYALALAFDLLPTAAQRRHAGNRLAQLAAEE
                     GYRIATGFVGTPLICDALTSVGAFDTAYHLLNQRECPSWLYPVTMGATTIWERWDSLL
                     PDGTINPGGMTSFNHYALGAVADWLHRTVAGLAPAAPGYRRILVSPRPGGGLTNAAAT
                     LLTPFGAAAVEWTRVGRTFSLTVTVPPGTTASVHLPDPAVEPVDVGSGTHTFSCEFRD
                     PVDDPPIPPKLNMHAQPIDEPVHVLGHAPLLDSQTA"
BASE COUNT          271 a          450 c          500 g          295 t
ORIGIN      
        1 aacttgtact cgagcagcat tcgctggtca gcaccaagcc gggcgtatac cttgcccaac
       61 gcatctgcga gtcgccgctg gcgggtcacg atgtcgtctt ggccgggata gttggtgcca
      121 tcggaaaacc acaacttcaa gtcacggcta ccggtcgcat ccatgatgtc gatgcaggcc
      181 agtaggtgat cggtcgcctt gcggcgtacg cgctcgctcg ggttggtcac cgaccccagc
      241 atgtagtcgt tgtcctgaaa cacattcgag ttgatggttc ctatcgccac ccctagatcg
      301 ttcgcgtggc gagacaactt cgagtagtca tcaactgcat cccaagggat gtgcaatgcc
      361 acggtcggcg cgacgccggt gaatcgatgc acctgtgccg catcggcgat cttctcccac
      421 ggatcccgag gtacgccctc ttgcgcgaac accttgaacc ttgttcccga gttcccgaac
      481 gcccacgagg gcaactcaat cgtctgtctc cgaagcgcag tcatggtgtc tcgcgtgtcg
      541 gtcgtcaaca ttggctctcc agatttcacg atgaaggtca tgctgtttgc gagtccaaga
      601 gcggggcgtg accgagtaca tgcactggct cgtcgatcgg ctgggcgtgc atgttgagct
      661 tcggagggat cggcggatcg tcgacagggt cgcggaactc gcacgagaag gtgtgcgttc
      721 cgctgccgac atcgacgggt tcgaccgccg gatcaggcag gtgcactgac gctgtagtac
      781 caggtggcac cgtcactgtg aggctgaacg tgcgtccaac ccgagtccac tcaaccgcgg
      841 ccgcgccgaa tggtgttagc agcgttgctg ctgcgttcgt gagcccgccg ccgggtcggg
      901 gggacacgag aatgcggcga tacccaggtg cggcaggtgc caagccggca accgttcggt
      961 gcagccagtc ggctactgca ccaagtgcgt agtggttgaa cgaggtcatg ccgccagggt
     1021 tgatggtgcc atctgggaga aggctgtccc atcgctccca gatcgtggtg gcacccatcg
     1081 tcaccgggta gagccacgat gggcactcgc gctggttcag caggtgatag gcggtgtcga
     1141 aggccccgac ggaggtcagt gcatcgcaga tcaatggcgt accgacgaat cccgttgcga
     1201 tccggtagcc ctcttcggcg gcgagctgcg cgaggcgatt accggcatgt cgtcgctgtg
     1261 cagcagtggg aagcaaatcg aacgcgagcg cgagtgcgta cgcggtctgc gagtcgctgc
     1321 taatcctgcc gctcggcgcc acgtattcgt ctgcgaacgc gacacggacc ttggcagcgc
     1381 gggtggcgta ccggcgctga tcgtcgttgt ggccgagtac acccgcgacg tcggagagga
     1441 cctgcgccgt tcgtgcgtga taggcagtcg caaccagata tcgatcggtg cgcgcgtcgt
     1501 cgggacgggc gggtgg
//
LOCUS       DASWVF010000459         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_139703, whole genome shotgun sequence.
ACCESSION   DASWVF010000459 DASWVF010000000
VERSION     DASWVF010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139703"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VGD55_08225"
     CDS             <1..1084
                     /locus_tag="VGD55_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY0870370.1"
                     /translation="FEVDDRELNATILPTDDHVSPDGRVMEVLSEHMCQGWLEGYLLT
                     GRHGLFNCYEAFIHIVDSMFNQHAKWLKTTRRIPWRRPIASLNYLLSSHVWRQDHNGL
                     SHQDPGFIDHVMNKKAEIIRVYLPPDANTLLSVADHCLRSRNYVNVIVAGKQPALNFL
                     SMDEAIAHCTRGIGIWEWASNDCGDTGTGEPDLVMACAGDVPTLEALAATALVREYFP
                     NLSVRFVNVVDLMRLQPDTEHPHGMSDREFDQLFTVDKPVVFAYHGYPWLIHRLTYRR
                     TNHANIHVRGFKEEGTTTTPFDMVMMNDLDRYHLVMDVIDRVPSLGSSAAHVRQRMVD
                     ERLHKRQYTREFGDDAPEIKNWTWPY"
     gene            1072..2106
                     /locus_tag="VGD55_08230"
     CDS             1072..2106
                     /locus_tag="VGD55_08230"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY0870371.1"
                     /translation="MAVLVRILVVNAGSSSLKLRLLGPGDVVEASWDALPDGLPDVDV
                     VVHRVVHGGTAFRGAVVIDEHVEATLQELIPLAPLHQPKTLAALAAVRKLLPDAVQVA
                     CFATAFHATIPEAAATYALPREWIAKYALRRYGFHGLSHAWASGRAKELVPGATRIIT
                     CHLGAGASLCAVLDGASVDTTMGFTPLEGLVMATRSGDVDPGLLMWLATREPDLADVL
                     DKRSGLTGLAGTGDMRALLARADHDARLAIEVYLHQLRKSIGAMATSLGGLDVCVFTG
                     GVGENAPDIRQRAVDGLRFLGLEIDATANSTVSGDADITASDAAARTLVIAAREDLEM
                     ARQAHQLLGI"
BASE COUNT          382 a          701 c          663 g          365 t
ORIGIN      
        1 cttcgaggtc gacgaccgtg agttgaacgc gacgatccta ccgaccgacg accatgtctc
       61 acccgatggc cgggtcatgg aggtgctgtc ggagcacatg tgccagggct ggctcgaagg
      121 ctacctgctc accgggcgcc acggcctgtt caactgctac gaggcgttca ttcacatcgt
      181 cgactcgatg ttcaaccagc acgcgaagtg gctgaagacg actcggcgca tcccgtggcg
      241 cagaccgatc gcaagcctca actacctgct gtcgagccac gtgtggaggc aggaccacaa
      301 cggtctgtcg caccaggatc ccggattcat cgaccacgtg atgaacaaga aggcggagat
      361 catccgcgtc tacctgccac ctgacgccaa cacgttgctt tcggtggccg accactgcct
      421 gcgcagtcgc aactacgtga acgtcattgt cgccggcaag cagcccgcac ttaacttcct
      481 ttcgatggac gaagcgatcg cccattgcac gcgcggcatc ggcatctggg aatgggccag
      541 caacgactgc ggcgacaccg gcaccggcga gcccgacctg gtgatggcgt gtgccggcga
      601 cgtcccgacg ttggaagcac tcgccgcgac cgctctggtg cgggagtact tcccgaacct
      661 gtccgtgcga ttcgtcaacg tggtcgacct gatgcggctc cagcccgaca ccgaacaccc
      721 gcatggcatg tccgatcggg agttcgacca actgttcaca gtcgacaagc cggtcgtatt
      781 cgcctaccac ggctacccct ggctgatcca ccggctgacc taccggcgaa ccaatcacgc
      841 caacatccac gtgcgcgggt tcaaggagga aggcacgacg accacgccct tcgacatggt
      901 gatgatgaac gacctcgacc gctatcacct ggtgatggac gtcatcgatc gggtgccgtc
      961 gttgggctcg agcgccgcgc acgtgcggca gcggatggtc gacgagcggc tccacaagag
     1021 gcagtacacg cgggagttcg gcgacgacgc ccccgagatc aagaactgga cgtggccgta
     1081 ctagtgcgga tcctggtcgt caacgccggg tcgagcagtc tgaagcttcg gctgctcggc
     1141 cctggcgatg tggtcgaggc atcgtgggac gcgttgcccg atggccttcc cgacgtcgat
     1201 gtggtcgtgc atcgggtcgt tcacggcggc accgcgtttc gtggcgcggt ggtcatcgac
     1261 gagcacgtcg aagcaaccct gcaggagctc attccgcttg ctccgctgca ccagccgaag
     1321 acacttgccg cgttggcggc cgtgcgaaag ctgctgcccg acgccgtgca ggtcgcgtgc
     1381 ttcgccaccg cgttccacgc cacgatcccg gaggcagccg cgacctatgc gctgccaagg
     1441 gaatggatcg cgaagtacgc cctgcgccgc tacggatttc acggtctctc gcacgcgtgg
     1501 gcaagcgggc gcgcaaagga gctagtcccg ggcgccacca ggatcatcac ctgccatctc
     1561 ggtgcaggcg cgtcgttgtg cgcagtactc gacggcgcat cggtcgacac cacgatgggg
     1621 ttcactcccc tcgaaggcct ggtgatggcg acccgttctg gcgacgtcga tcctggcctc
     1681 ctgatgtggc ttgctacccg cgaaccggat ctcgccgatg ttctcgacaa gagatcggga
     1741 ctcaccgggc tcgcgggcac cggtgacatg cgcgccctgc tcgcgcgagc cgatcacgat
     1801 gcgcgactcg ctatcgaggt ctacctgcac caactacgca agagcatcgg cgccatggcg
     1861 acctcgctgg gcggtctcga cgtgtgcgtc ttcaccggtg gagtcggtga gaacgccccc
     1921 gacatcaggc agcgcgcggt cgacgggctg cgatttctcg ggctcgagat tgacgccacc
     1981 gcgaacagca ctgtttcagg ggacgccgac atcacggcgt ccgatgcagc tgcgcgcacg
     2041 ctcgtcatcg ctgcacgcga ggatctcgag atggcgcggc aggcacatca actgctcggg
     2101 atctagctcc t
//
LOCUS       DASWVF010000460         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_292355, whole genome shotgun sequence.
ACCESSION   DASWVF010000460 DASWVF010000000
VERSION     DASWVF010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292355"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1258
                     /locus_tag="VGD55_08235"
     CDS             164..1258
                     /locus_tag="VGD55_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870372.1"
                     /translation="MRSTPAEPERPSATAAVLATVAAALETGAVLLEDDDVLLANEAA
                     LALRVVYGRELTSRPLARIAREARRTGVRIAQDLDLPWGSSSRAVHVVAAPVPGTAQV
                     VLLMNDLEESRRLEAVRRDFIANVSHELKTPVGAMLLLAEALRDATDDPVAMKQFTER
                     MLHEAGRMSRLVQELLELSRLQGGEPLPAMESIRVSDVIADATDPLQVRAEAAGITLD
                     VAPANGSQVMGDRRQLTTALTNLIENAIAYSSPGSRVGVGARTFVEGGEPGVEITVTD
                     EGVGIEKVDQERVFERFYRVDTARSRATGGTGLGLAIVKHIANNHGGRVSLWSHPGVG
                     STFTLYLPVPPTNTTATTNGGNPHQKGQES"
     gene            1255..>1845
                     /locus_tag="VGD55_08240"
     CDS             1255..>1845
                     /locus_tag="VGD55_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870373.1"
                     /translation="MTHLLVIEDEESFSETLAYMLTREGFEVRVATTGPAGLEEFDRH
                     GADLVLLDLMLPGLPGTEVCRRLRERSDVPVIMLTARDSEIDKVVGLELGADDYVTKP
                     FSARELVARIRAVLRRRSDTAEAVVSGIVEAGPVRLDPDRHVVSVRGETITLPLREFE
                     LLELLLRNAGRVLTRNQLIDRLWGSDYVGDTKTLDVP"
BASE COUNT          332 a          588 c          596 g          329 t
ORIGIN      
        1 cgggacgaat actcccacgt acccctacgt ccctggcaga gtctgtcacc tgaatggacg
       61 gcggcgggca acaaggcgac acagtgcaga acgcaaggtg aacagtcggc caacgggtcg
      121 gcttagtttg gtgactttcc cacaggtgcc tacgattcgg gttgtgcggt cgactcctgc
      181 ggagcctgaa cggccgtcgg cgacggcggc ggttctcgca accgtcgccg cagcccttga
      241 gaccggtgcg gtccttcttg aggacgacga tgtgttgctc gccaacgagg ccgcgctagc
      301 gctgcgggtc gtctacgggc gcgagctaac ctcccgcccg ctggctcgga tcgcccgcga
      361 ggccaggcgt accggcgtca ggatcgccca ggatctcgac ctgccgtggg gctcgtcgtc
      421 ccgagcagtg cacgtcgttg cggcgccggt gcctggcact gcgcaggtcg tgttgctgat
      481 gaacgacctc gaggaatccc gccgacttga agcagtgcgc cgcgacttca tcgcgaacgt
      541 ctcccatgag ctgaagacgc cggtcggcgc gatgctgctg ctggcggaag ccctgcggga
      601 tgccactgac gatcccgtcg ctatgaagca gttcaccgaa cgcatgctgc acgaggcggg
      661 tcgcatgtcc cgactcgtgc aggagttgct cgaattgtct cgccttcagg gcggcgaacc
      721 gctgccggca atggagtcga tccgagtgag cgacgtcatc gccgacgcga ccgatccgct
      781 tcaagtgcgg gccgaagcgg ctggcatcac gctagacgtc gctcctgcga acgggtcgca
      841 ggtgatgggc gatcgccgtc aactgaccac ggctctcacg aacctcatcg agaacgccat
      901 cgcctactcc tcgcccggat cgcgggttgg ggtgggtgcg cgcaccttcg tcgaaggtgg
      961 cgagcccggc gtcgagatca cggtcaccga cgaaggcgtc ggcatcgaaa aggtcgatca
     1021 agaaagggtg ttcgaacggt tctaccgagt agacacggcg cgatcgcgcg cgaccggcgg
     1081 aaccggtctc ggcttagcga tcgtcaagca catcgcgaac aaccacggcg gccgagtgag
     1141 cctttggagt catccaggag tcggttcgac ctttacgttg tacctgcccg tgcctccaac
     1201 gaacaccaca gccaccacca acggcggtaa cccgcatcag aaaggccagg agtcgtgacg
     1261 catctgctcg tcatcgagga cgaggagtcg ttctccgaga cgctcgcgta catgctcacc
     1321 cgcgaaggat tcgaggtacg cgtcgcaacc accgggccag ccgggctcga agaattcgat
     1381 cggcacggtg ccgacctcgt tcttctcgac ctcatgctgc ctgggctccc gggtaccgag
     1441 gtgtgccgca gacttcgcga gcgcagcgac gtgcccgtca tcatgctgac tgcccgtgac
     1501 agtgagatcg acaaagtcgt cggcctcgag ttgggtgccg acgactacgt gaccaagccg
     1561 ttctccgcgc gcgaactcgt cgctcgcatc agggcggtgc tgcgccggcg cagtgatacg
     1621 gcagaggctg tcgtgtcggg catcgtcgaa gccggacctg ttcgactcga ccccgaccgc
     1681 catgttgtga gcgtgcgtgg tgaaaccatc acgttgccgt tgcgcgagtt tgaattgctc
     1741 gagttgttgt tacgcaacgc tggtcgagtt ctgacacgca accagctcat cgaccggttg
     1801 tggggcagcg actatgtcgg tgacaccaag acactcgacg tccct
//
LOCUS       DASWVF010000461         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_216149, whole genome shotgun sequence.
ACCESSION   DASWVF010000461 DASWVF010000000
VERSION     DASWVF010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216149"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VGD55_08245"
     CDS             complement(<1..1463)
                     /locus_tag="VGD55_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0870374.1"
                     /translation="MITATDVEISVGSRVLLDAVTFRVAPGDRIGLVGRNGAGKTTLT
                     KVLAGEAIPASGTVTTTGQIGYLPQDPRTGDLEILARDRILSARGLDVVVRDMRAAED
                     KMGSTDDSVRDAAVRRYSRIEDHFHVLGGYAAESEAASIAASLGLPDRVLGQSLGTLS
                     GGQRRRVELARILFSGAETLLLDEPTNHLDADSIAWLREFLRSHKGGLVLISHDVTLL
                     ETTVNRVLHLDATRAVLDIYNVGWKTYLDQREADERRRKRERANAERKATTLQAQADR
                     MRYKATKATAAQNMEKRAHKLLAGLEEVRKHDRVAKLRFPDPAPCGKVPLSATGLSKS
                     YGSLEVFTDVDLAIDKGSRVVILGLNGAGKTTLLRTLAGVETADTGEVLAGHGLKLGY
                     YAQEHETLDPDATVLETMRASAPDLADVELRRVLGSFLFSGDTVEQRTKTLSGGEKTR
                     LALARLVVSSANVLLLDEPTNNLDPASREEVLGALRG"
     gene            complement(1476..1787)
                     /locus_tag="VGD55_08250"
     CDS             complement(1476..1787)
                     /locus_tag="VGD55_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY0870375.1"
                     /translation="MTTTVEDVKEALYDVVDPELGINVVDLGLIYDLALDADGIATID
                     MTLTSAACPLTDVIEDQTRSALDGLVKDFKINWVWMPPWGPEKITDDGREQLRAIGFN
                     V"
     gene            complement(1784..2290)
                     /locus_tag="VGD55_08255"
     CDS             complement(1784..2290)
                     /locus_tag="VGD55_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY0870376.1"
                     /translation="MQLESMYQEIILDHYRTPHHKGLREPYDVEVHHVNPTCGDEITL
                     RVKLDGDTVTDVSYDSVGCSISQASASVLTDLVIGHGIDTGLVAHREFVALMQSKGTA
                     TPDEEILEDAVAFTGVSKYPARVKCALLSWMAWKDATAQAQARTSPVENSSSADTALV
                     NTPSERLS"
     gene            complement(2290..>3397)
                     /locus_tag="VGD55_08260"
     CDS             complement(2290..>3397)
                     /locus_tag="VGD55_08260"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEY0870377.1"
                     /translation="VVITEMEHHSNIHRATHALGEEATAAYEGARMKVAAFIGATDTD
                     EVIFTKNSSESLNLVANVLSWGARALKPGDEVVITEMEHHSNIVPWQILCERTGAMLR
                     WFGVTDEGRLDLSNIDELINERTKVVSFVHVSNILGTLNPVQRIAARAHEVGALVVLD
                     ASQSVPQLPVDVHALGIDFLAFTGHKMCGPTGIGVLWGRRGVLEELPPFLGGGEMIET
                     VTMQRSTYAGLPHKYEAGTPPIAEAVALGAAVDYLTNLGIDAVAAHEQQITAYALDAL
                     SGVSGLRVLGPTNMIDRGGAISFTLGDVHPHDVAQVLDGHGVAVRAGHHCGRPIHQRF
                     GVSASTRASFYLYTTLPEIDALVDGLEKAKRFFG"
BASE COUNT          565 a         1087 c         1107 g          638 t
ORIGIN      
        1 tagccgcgca gcgcgccgag aacctcttcc ctgctcgcgg ggtcgaggtt gttcgtcggc
       61 tcgtcgagca acagcacgtt ggcgctcgag acgacgaggc gcgcgagtgc caggcgggtc
      121 ttctcaccac ccgataacgt cttcgtgcgc tgttctaccg tgtcgcccga gaacaggaag
      181 ctaccgagga cgcggcgcaa ttcgacgtcc gccaaatctg gtgccgaggc ccgcattgtc
      241 tcgagcacag tggcatcggg gtcgagtgtc tcgtgctctt gtgcgtaata gccgagcttg
      301 aggccgtggc ccgcaagcac ctcgcccgtg tcggccgttt cgacgcccgc gagggtgcgc
      361 aacagtgtcg tcttgccggc gccgttcaag ccgagaatca ccacgcgcga acccttgtcg
      421 atcgcgaggt cgacgtcggt gaagacctcg agcgaaccgt acgacttcga caaaccggta
      481 gccgataacg gcaccttgcc gcacggtgca ggatcgggaa aacgcagctt cgcgacgcgg
      541 tcgtgtttac gcacttcttc caagccagca agcaatttgt gcgcccgctt ctccatgttc
      601 tgcgcggccg ttgccttggt cgccttgtag cgcatgcggt cggcctgcgc ctgcaacgta
      661 gttgcctttc gctcggcgtt cgcccgctct cgcttgcgtc gccgttcgtc tgcttcacgc
      721 tggtcgaggt aggtcttcca gccgacgttg tagatgtcga gcacggcgcg agtcgcgtcg
      781 agatgaagaa cgcggttcac cgtcgtctcg agcaaggtca catcgtggct gatgagcacc
      841 aggccgccct tgtgtgagcg aaggaactcc cgcaaccagg cgatggagtc agcgtcgagg
      901 tggttggtgg gttcgtccag cagcagcgtc tcggcgccag agaacaagat gcgcgcgagt
      961 tcgacacggc ggcgctgccc gcccgacaac gtgccgaggg actggccgag cacccgatcg
     1021 ggcagcccga ggctcgcagc gatcgatgcg gcctccgatt cggccgcata gccgccgagg
     1081 acgtggaagt ggtcctcgat gcggctataa cgccgaacag cggcgtcgcg cacggagtcg
     1141 tcggtgctgc ccatcttgtc ttcggctgcc cgcatgtcgc gcacgacgac gtcgagcccg
     1201 cgtgccgaca ggatccggtc gcgcgccaga atctcgaggt cgcccgtgcg cgggtcttgc
     1261 ggcaggtagc cgatctgacc ggtcgtcgtg acagtgccag acgccggaat tgcctcgccc
     1321 gcaagcactt tggtcagtgt cgtcttgccc gcgccgttgc gcccgacaag accgatgcga
     1381 tcacccggcg cgaccctgaa cgtgaccgcg tcgagcagca ctcgtgagcc gacgctgatt
     1441 tcaacgtcgg tagcagtaat caccgttaga ccgcgtcaga cattgaatcc gatggcgcgc
     1501 aactgctcgc ggccgtcgtc cgtgatcttc tctggtcccc acggcggcat ccagacccag
     1561 ttgatcttga agtctttcac cagcccgtcg agcgcgctgc gagtctggtc ttcgatgaca
     1621 tcggtcagcg ggcacgccgc cgaggtgagc gtcatgtcga tcgtcgcgat gccgtcggcg
     1681 tcgagggcga ggtcgtagat gagtccaagg tcgacgacgt tgatgccgag ctcggggtcg
     1741 acaacgtcgt acagagcttc cttgacgtct tcaacggtgg ttgtcatgac agcctttcgg
     1801 acggcgtgtt gacgagcgcg gtgtcggcac tcgaggagtt ctcgaccggt gaggtgcgcg
     1861 cctgggcttg cgccgtggcg tctttccagg ccatccatga caacagcgcg cacttgaccc
     1921 gcgccgggta tttcgagacg ccggtgaacg cgacggcatc ttcgaggatc tcttcgtcgg
     1981 gagtcgcggt gcccttgctc tgcatcaatg cgacgaactc gcggtgcgcg actaacccgg
     2041 tgtcgatgcc gtggccgatc accaggtcgg tcagcaccga cgcactggct tggctgatcg
     2101 agcagccgac gctgtcgtac gacacatcgg taacggtgtc gccgtcgagc ttgacgcgca
     2161 acgtgatttc gtcgccgcag gtcgggttga cgtggtgcac ctcgacgtcg tagggctccc
     2221 gcaaaccctt gtggtggggc gtgcggtagt gatcgaggat gatttcttgg tacatcgatt
     2281 cgagttgcat cagccgaaga acctcttcgc cttctcgagc ccgtcgacca gcgcatcgat
     2341 ctcgggcagc gtggtgtaga ggtagaacga cgcgcgggtc gatgcgctga caccgaaccg
     2401 ctgatggatc ggccgcccgc agtgatgacc cgcgcgcaca gcgactccgt gcccgtcgag
     2461 cacctgcgcg acgtcgtgcg ggtgcacgtc gccgagagtg aacgagatcg cgccaccgcg
     2521 gtcgatcatg ttggtcggac cgagaacgcg caacccgcta acgccactca gtgcgtcgag
     2581 cgcgtaggcc gtgatctgct gctcgtgggc cgctacggcg tcgatgccca gattcgtcag
     2641 gtagtcgact gccgcgccga gcgcgaccgc ttctgcgata ggcggcgtgc cggcttcgta
     2701 cttgtggggc aggccggcgt aggtcgaacg ctgcatcgtc actgtctcga tcatctcgcc
     2761 accaccgagg aacggcggca actcttcgag caccccgcgc ctgccccaca agacgccgat
     2821 gcccgtgggt ccacacatct tgtgccctgt gaacgcaaga aaatcaatac caagcgcgtg
     2881 gacgtcgacg ggcagctgtg gcaccgactg cgacgcatcg agcaccacaa gggcaccgac
     2941 ctcatgcgcg cgcgccgcga tgcgctgcac cgggttcaag gtaccgagga tgttcgagac
     3001 gtgcacgaac gacacgacct tcgtgcgctc gttgatgagc tcatcgatgt tggacagatc
     3061 gaggcgaccc tcgtcggtga ccccgaacca gcgcagcatc gcgccggtgc gctcgcacag
     3121 gatctgccac ggcacgatgt tggagtggtg ctccatctcg gtgatgacga cctcgtcgcc
     3181 gggcttcagc gctcgggcgc cccacgacag cacgttcgcg accaggttca gcgactcgct
     3241 ggagttcttg gtgaagatga cctcgtcggt gtcggtcgcg ccgatgaacg cggcgacctt
     3301 catgcgggcg ccttcgtagg ccgccgttgc ctcttcgccc aacgcgtgtg tcgcgcggtg
     3361 gatgttggag tggtgctcca tctcggtgat gacgacc
//
LOCUS       DASWVF010000462         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_470966, whole genome shotgun sequence.
ACCESSION   DASWVF010000462 DASWVF010000000
VERSION     DASWVF010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470966"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VGD55_08265"
     CDS             <1..584
                     /locus_tag="VGD55_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0870378.1"
                     /translation="RGGTVDVEPDLSNAAPFLAAALVTGGTVHIPQWPEQTLQAGDAI
                     CRLLVQMGADISTDAHGLTIRGSGTIHGVDEDLRDTSELVPTVAVLAALADRPSTLRG
                     IGHMRGHETDRLAALAKEINGLGGDVTETPDGLHINPKPLHGGVFGTYNDHRLATSAA
                     VLGLATPGVLVENIATTTKTMPTFVELWSTLLR"
     gene            592..1614
                     /gene="rsgA"
                     /locus_tag="VGD55_08270"
     CDS             592..1614
                     /gene="rsgA"
                     /locus_tag="VGD55_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY0870379.1"
                     /translation="MSRNPRDLDEDDVRVRPGRSTRPRSRLRPAHDEATAAFVLAVDR
                     GRFTCEVGDHEVTAVRARELGRKSVVVGDRVALVGDVSGTTDTLARLVRIEPRTSVLR
                     RSADDSDPIERIIVANAEQLAIVCAVADPEPQPRLIDRYLVAAFDGGLQPLLVLTKAD
                     LADPAPLIEAYALLDLRVVVTHDGQPSDELRAVLRDRLTVLAGSSGVGKSTLINALVP
                     GAGRATGEVNAVTGRGRHVSTSAVVLDLPDGGRVIDTPGIRSFGLAHVDVDRVVAAFP
                     DLAPGTADCPRGCQHVAPDENAPAVDAEIAVCALDEWARTGHAEPARLDSLRRLLAVR
                     DKPDAD"
     gene            complement(1621..2190)
                     /locus_tag="VGD55_08275"
     CDS             complement(1621..2190)
                     /locus_tag="VGD55_08275"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY0870380.1"
                     /translation="MTTDDSTPDAASVLALLVAGDRLPVVAALVLGAQTIDDIAARTD
                     VPARDVLRVLTRLELAGLTSVDGSRWTLHTEILREVVTGHHWPPAEHAPSGESFGSSP
                     AIAAVLRAFFRGGRLVRVPAQRTKRLVVLDRLARDFEPGIRYPEVDVNELLLAYYPDY
                     AALRRYLVEEGYLARENGVYWRTGGSVEL"
     gene            complement(2204..2749)
                     /locus_tag="VGD55_08280"
     CDS             complement(2204..2749)
                     /locus_tag="VGD55_08280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0870381.1"
                     /translation="MRWTVHGERTLYDSAWVRLALVDVEIPGEDGRRFEHHVLRMPKP
                     ASGVVVYDVGRGVLLLWRHRFITDTWGWEVPAGRAEAKETSQQAATREVLEETGWRPG
                     PLRELASYHPNNGMSDMTFNLFVADGAEYVGPPTDPSESERIEWLPVSVVRELLRTGE
                     VRDGLSLTALSLALLFELPAQ"
     gene            2775..3581
                     /locus_tag="VGD55_08285"
     CDS             2775..3581
                     /locus_tag="VGD55_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0870382.1"
                     /translation="MPDARPPDDLTLALLLADAADEISAARFGAIDLVVDTKPDLTPV
                     SDADRAVETVIRTMLAHARPRDAVVGEEFGALANPDSAGRRWVVDPIDGTKNFIRGVP
                     VWATLIALMEGADVLVGVVSAPALSQRWWAARDYGSWTSRPGIAARRNRVSAVASLDD
                     ASLSYSDLGDWGSRSPGFHDLAESVWRTRAYGDFWSHMLVAEGAVDISAEPEVSIWDV
                     AALVVIVEEAGGRVTGVDAAPSPDAPSIVCTNGLLHEAVLATLLPSDTAS"
     gene            3805..4197
                     /locus_tag="VGD55_08290"
     CDS             3805..4197
                     /locus_tag="VGD55_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0870383.1"
                     /translation="MRVRDGMNPDVLTIGPSHTLREASHLMAKRHVGAAVVADADGLG
                     ILTERDVLESVGADEDPDIELVGDHLTKDLVFAAPDWNMDQAAAAMVRGGFRHLVVLD
                     GNDVVGILAMRDIVRSWIAIASSTGAAR"
BASE COUNT          744 a         1510 c         1438 g          730 t
ORIGIN      
        1 tgcgtggcgg cacggtcgac gtcgagcccg acctgtcgaa cgccgccccg tttcttgctg
       61 ccgcgcttgt cacgggcggt actgtgcaca taccgcagtg gccggagcag acgctccagg
      121 cgggcgatgc gatctgccga ctgctggtgc agatgggcgc cgacatcagc acagacgctc
      181 acgggctcac gattcgcggc agcggcacga tccacggcgt cgacgaagat ttgcgcgaca
      241 cgtccgagct cgtaccaacc gttgccgtgc tggccgcgct cgcggatcga ccctcgactc
      301 ttcggggcat cggtcacatg cgcgggcacg agaccgaccg gctcgccgcg ctggcgaaag
      361 agatcaacgg gctcggcggc gatgtcaccg agacacccga tggcctgcac atcaacccga
      421 aacctttaca cggtggggtt ttcggtacgt acaacgacca ccggctggca acctcagcgg
      481 ccgtgctagg gctcgccacg cccggcgtcc tggttgagaa catcgcaacc accacaaaga
      541 ccatgccaac gttcgtcgag ctctggagca ccctccttcg atgatgttgc cgtgagccgg
      601 aatccgcgcg acctcgacga ggacgacgtt cgcgtgcgcc ccggtcgcag cacgcgacca
      661 cgcagccggc tgcgtccggc acacgacgaa gcgaccgctg catttgttct cgcggtcgat
      721 cgaggccggt tcacctgcga ggtcggcgat cacgaggtca ctgcggtgag agcccgcgag
      781 cttggccgca agtcagtcgt cgtcggcgac cgggtcgcgc tggtcggtga cgtgtcgggc
      841 acgaccgaca cgctcgcgcg actcgtgcgc atcgagcccc gcacctctgt gctacgccga
      901 agtgccgacg acagcgatcc gatcgaacgc atcatcgtcg ccaatgccga gcagttggca
      961 atcgtgtgtg ccgtcgccga tccggagccg cagccgcgcc tgatcgaccg gtacctcgtc
     1021 gcggcattcg acggcgggtt acagcctttg ctcgtgttga ccaaggccga cctcgccgac
     1081 cctgcccccc tcatagaggc atacgcattg ctcgacctgc gggtcgtcgt cacccacgat
     1141 gggcagccaa gcgatgagct gcgggcggtc ctgcgcgacc ggctcactgt gctcgcaggt
     1201 agctcgggcg tcggcaagtc gaccctgatc aacgcactcg tcccaggcgc ggggcgggcg
     1261 accggtgagg tcaacgcggt caccggtcgt ggccggcacg tgtcgacctc ggcggtggtg
     1321 ctcgacctgc ccgacggtgg tcgcgtcatc gacaccccag gcatcagatc gttcggcctc
     1381 gcgcacgttg acgtcgatcg ggtggtcgcg gcgtttccag acctcgctcc gggcacggcc
     1441 gactgcccgc gcggctgcca acatgtggca ccggacgaaa atgcgcctgc tgtcgacgcc
     1501 gagatcgcgg tgtgcgcgct cgacgagtgg gcgcgaaccg gccacgccga acccgcccgg
     1561 cttgactcat tgcgccggct cctcgccgtc cgcgataagc ctgatgctga ctgacaagcg
     1621 tcagagctcg acactgccgc cggtgcgcca gtacacgccg ttctcgcgag cgaggtatcc
     1681 ctcttcgacc aggtatcggc gcagggcggc gtagtcgggg tagtaggcca gcaacagctc
     1741 gttgacgtcg acctccgggt agcgaatccc gggttcgaaa tcgcgggcga gccggtcgag
     1801 cacgacgagc cgcttcgtgc gttgcgcagg cacccgcacc agccgaccgc cgcggaagaa
     1861 agcgcgcagc acggcggcaa ttgccggcga cgaaccgaag ctttcaccgg acggcgcatg
     1921 ctcggcgggt ggccagtggt gaccagtcac cacctctctc aaaatctcgg tgtgcaaggt
     1981 ccatcgcgaa ccgtcgaccg acgtgagacc ggccaactcg agcctcgtca gtacgcgcaa
     2041 cacgtcgcgg gccggcacgt cggtgcgggc cgcgatgtcg tcgatcgtct gcgctccgag
     2101 cacgagcgct gcgacgacgg gcagccgatc gccagcgacc aacagcgcta acacactcgc
     2161 tgcgtcgggc gtcgagtcgt cggtggtcac tactgcaatt gccttactgc gcaggcaatt
     2221 cgaagagcaa cgccagcgaa agcgcggtca agctgaggcc atcgcgcact tctcccgtac
     2281 ggagcagctc acgcacaacc gaaacgggca gccactcgat gcgctccgac tcactcgggt
     2341 cggtgggggg tccgacgtac tcggcgccat cagcgacgaa gaggttgaag gtcatgtcgc
     2401 tcatgccgtt gttggggtgg tagctcgcga gttcgcgcaa cggcccgggc cgccacccgg
     2461 tctcttcgag cacttcgcgc gtcgccgcct gctgtgatgt ctccttcgct tctgctcgac
     2521 cggccggaac ctcccaaccc caggtgtcgg tgataaagcg atgccgccaa agcagcaaca
     2581 ccccgcgccc gacgtcgtac accacgacgc cacttgccgg tttcggcatc cgcagaacgt
     2641 ggtgctcaaa gcgtcggccg tcctcgccgg ggatctcgac gtcgacaagc gcgagacgca
     2701 cccacgcgct gtcataaagg gtccgctcac catgaaccgt ccagcgcatg catcgaccgt
     2761 atagcgtgcc gaccatgccc gacgcgcgcc cgcccgacga cctgacgttg gcgctcctcc
     2821 ttgcggatgc ggccgacgag atcagcgctg cacgattcgg agcgatcgat ctcgtcgtcg
     2881 acaccaagcc cgatctcacc cctgtcagcg acgccgaccg cgcggtcgag acagtgatcc
     2941 ggacgatgtt ggctcatgcg cgcccgcgag acgcagtcgt cggtgaggag ttcggggccc
     3001 tggctaaccc cgattcagct ggacgccgat gggtggtcga tccgatcgac ggcaccaaga
     3061 acttcattcg aggagtgccg gtctgggcca cgctgatcgc gctgatggaa ggcgccgacg
     3121 tcctggtcgg ggtcgtcagc gcgcccgccc tgagtcagcg ctggtgggcc gctcgcgact
     3181 acggcagctg gacgtcgcgg cccgggattg ccgctcgccg caatcgagtc tcggcagtcg
     3241 cgtcactcga cgatgcgtcg ttgtcgtact ccgacctcgg cgattggggc tcgcgctcgc
     3301 ctgggttcca cgacctcgcg gagtcggtct ggcgaacccg cgcgtacggg gatttctggt
     3361 cgcacatgct ggtcgccgaa ggcgcggtcg atatctcggc ggagcctgag gtgtcgatct
     3421 gggacgtcgc ggcactggtc gtcatcgtcg aagaggcagg cggccgcgtt accggcgtcg
     3481 acgcggcgcc gagccccgat gcacccagca tcgtgtgcac caacggctta ctccacgaag
     3541 ccgtgctggc gactttgttg cccagcgaca ccgcgtcgta gtccagcacc gctatctcgg
     3601 cacaagagtg acggggcatc ggatcccgct ggctgtggca agtcgcgcgt cggcggtgac
     3661 gagcccgcag tcgagttgct ccgccagcgc gacgttcacc acaccgaccg gggtaccagt
     3721 ccttgtggga gcgcttccgg gggtccacga tgaggcgagg tgcccctttg cgcccttgtg
     3781 caccctgcgc ctttggaggc tgcgatgcgt gttcgggacg ggatgaaccc cgacgtgttg
     3841 acgatcgggc caagccacac gctgcgagaa gccagccacc tgatggcgaa acggcacgtc
     3901 ggcgctgcgg tcgtggccga cgcggacggg ctcggcatcc tcaccgaacg cgacgtcctt
     3961 gagtcggtcg gcgcagacga ggatcccgac atcgagcttg tcggagatca cctcacgaag
     4021 gacctggttt tcgctgcacc cgactggaac atggaccagg cagcagcggc catggtgcgc
     4081 ggcggcttcc ggcacctcgt cgtgctggat ggaaatgacg tcgtcgggat cctcgccatg
     4141 cgcgacatcg tgcgcagctg gatcgcgatc gcgtcctcga ccggagctgc tcgctagtca
     4201 ggttccgaca gtcgcattca gtcagattcc gacagtcgca ttcagtcaga ttccgacagt
     4261 cgcattcagt cagattccga cagtcgcatt cagtcaggtt ccgacagtgt gaccctgatc
     4321 gctcgacacg cctcaaggct gtcgcgcaac acctgcgccc ttggatctgg cacgtcgagg
     4381 attcgctcgg taaccaacgg ccgcaacgca gccagccgtg cg
//
LOCUS       DASWVF010000463         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_496368, whole genome shotgun sequence.
ACCESSION   DASWVF010000463 DASWVF010000000
VERSION     DASWVF010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496368"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VGD55_08295"
     CDS             <1..1046
                     /locus_tag="VGD55_08295"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY0870384.1"
                     /translation="FQQDAVGSPRDGWEYSRSGNPTRAALEQCLAALEGGARGLAFAS
                     GLAAEDCVLRTLCAPYDHVVVPGDAYGGTFRLVDKVFGRWGVAHASVDLSDLDALARA
                     MTPKTTVVWCETPSNPLLGIADIAAIAQIAHEAGAKLVVDNTFASPYLQQPLSLGADI
                     VVHSTTKYIGGHSDVVGGALVAADVGLGEQLAFHQNAIGSIAGPFDSWLVMRGVRTLA
                     VRMDRHCENAVRIANFLAHHRAVSQVLYPGLPTHAGHEIAAKQMRAFGGMVSFRMDAG
                     EAAAIDVCNRARLFTLAESLGGVESLIEHPGRMTHVSVVGSPLEVPSDLVRLSVGIEN
                     VDDLLADLEQALA"
     gene            complement(1043..2296)
                     /locus_tag="VGD55_08300"
     CDS             complement(1043..2296)
                     /locus_tag="VGD55_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870385.1"
                     /translation="MHRLRAKVFGDLPNEVAVLAAVAFSVAVGFGIVAPVIPLFAHHF
                     GVGRAEVGAVLSAFAFMRLASALGVGRLVDAIGERVVLATGIGIVAVSSALAGFAHSY
                     LQLLLLRGAGGIGSAMFSVSAMGLLLRVVGPEQRGRSVGLFQGGFLLGGISGPAIGGP
                     LAAWSIRAPFFVYAGTLVVAGTVAMVGLRRASLARAPDPAHAGHPGRTSLTAALRQRT
                     YRAALVANLADSWAAMGVRNTLVPLFVVESLHRGTAWVGIGFTVVAAFNACTLIPAGR
                     LVDAHGRRPIIVAGCLLSGSSMALLAVSPNIGGYLIAMVVFGVGSGLLDVAPAAVVGD
                     VAGKRGGTVVAAFQMAGDGGSVSGPVVAGWVADTWSYGAAFWVTTGILAGAATLAARA
                     PDVKTGEPAPLAEAEIRADLSAEKA"
BASE COUNT          399 a          829 c          834 g          352 t
ORIGIN      
        1 cgttccagca ggacgcggtg ggaagtccac gcgacggctg ggagtacagc cgctccggca
       61 acccgacccg cgcggcgctt gagcagtgcc tggccgccct cgaaggtggc gcgaggggtc
      121 tcgccttcgc gagtggtctg gccgctgaag actgcgtcct gcgcacgttg tgcgcgccct
      181 acgaccatgt cgtcgtgccg ggagatgcgt acggcggcac gttccggttg gtcgacaagg
      241 tgttcggtcg ttggggggtt gcccacgcgt cggtcgacct atcagacctc gacgcgctcg
      301 cacgtgccat gaccccgaaa accacggtgg tgtggtgtga gacgccgagc aatccgctgc
      361 tcggcatcgc cgacatcgcc gcgatcgcgc agatcgctca tgaggcaggc gcgaagctgg
      421 tcgtcgacaa caccttcgcc tcgccctatc tccagcagcc tttgtcattg ggcgccgaca
      481 tcgtggtgca ctccacgacg aagtacatcg gcggccactc cgatgtcgtg ggcggtgcgc
      541 tggtcgcggc cgacgtgggt cttggcgagc aactcgcgtt ccatcagaac gcgatcggtt
      601 cgatcgccgg cccgttcgac tcgtggctgg tgatgcgcgg cgttcgcacc ctcgccgtcc
      661 gcatggatcg gcactgcgag aatgccgtca ggatcgcgaa cttcctcgcg catcaccggg
      721 ccgtttcgca ggtgctgtat ccgggtttgc cgactcacgc cggtcatgag atcgctgcga
      781 aacagatgcg cgcattcggc ggcatggtgt cttttcgcat ggacgccggg gaagcggccg
      841 cgatcgacgt gtgcaaccgc gcacggctgt tcacgcttgc cgaatcgctt ggcggggtcg
      901 agtcgctgat cgagcatccg gggcgaatga cccacgtcag cgtggtcggg agtccgctgg
      961 aagtgccgtc ggacctggtt cggctgtcgg tcggtatcga aaacgtcgac gatctgcttg
     1021 ccgatctcga acaggctctg gcctaggctt tttcggcgct gaggtcagct cggatctcgg
     1081 cctctgctag cggcgctggc tcgcccgtct tgacgtcggg cgcgcgggca gccagcgtcg
     1141 ccgcgccggc caggatgccg gtcgtcaccc agaacgcagc gccatacgac caggtgtcgg
     1201 cgacccatcc ggcgacgaca gggccgctca ccgacccccc gtcgccggcc atctggaacg
     1261 cggccaccac cgtgccaccg cgcttgcccg cgacgtcgcc gacgacggcg gctggcgcga
     1321 cgtcgagcag gcccgacccg actccgaaca cgaccatcgc gatcaggtaa cccccgatat
     1381 tcggcgacac ggccagcagt gccatcgagc tgcctgacag caagcaacca gcgacgatga
     1441 tcggacgccg accgtgcgcg tcgacgagcc gaccggccgg gattagcgtg caggcgttga
     1501 atgcggcaac cacggtgaat ccgatgccga cccacgcagt accacggtgc agcgattcga
     1561 cgacgaagag cggtaccagc gtgttgcgta cgcccatagc cgcccaactg tccgcgaggt
     1621 tcgcgacgag ggcggcccgg taggttcgct ggcgcagggc ggccgtgagg ctggtgcggc
     1681 cggggtgtcc ggcgtgcgcg ggatcgggtg cacgcgcgag agatgcgcgc cgcagaccga
     1741 ccatcgcgac ggtgccggcg accacgagcg tgcccgcgta gacgaagaac ggcgcgcgga
     1801 tcgaccacgc ggcgagcggg ccgccgatcg cggggcccga gatgccgccg agcaggaagc
     1861 cgccttggaa gagcccgacc gagcggccgc gctgctcggg cccgacgacg cgcagcagga
     1921 gtcccatcgc actgaccgag aacatcgcag agccgatgcc gcccgcgcct cgcagcagta
     1981 gcaactgaag gtagctgtgc gcgaagccgg cgagggcgct gctgacggcg acgatcccga
     2041 tgccggtcgc aaggacgacg cgttcgccga tggcgtcgac gaggcgcccg acgcccagtg
     2101 ctgaggccag ccgcatgaac gcgaacgccg acagcaccgc accgacctcg gcccggccga
     2161 cgccgaaatg gtgagcgaac aacgggatga ccggcgcgac gatgccgaat ccgaccgcaa
     2221 cgctgaacgc gacagccgcg agcacggcca cttcgttcgg aagatcgccg aacaccttcg
     2281 cgcgcaaccg gtgcatccgg atcacgctac ccgctcgccg cgccggcgca gctcagccgt
     2341 gggctcagcc gtgacggccc cggcgcgccc gcagatagtc gctgacgacg tagtcgccga
     2401 gcttgtcgtc ggcc
//
LOCUS       DASWVF010000464         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_88654, whole genome shotgun sequence.
ACCESSION   DASWVF010000464 DASWVF010000000
VERSION     DASWVF010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88654"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1398
                     /locus_tag="VGD55_08305"
     CDS             184..1398
                     /locus_tag="VGD55_08305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0870386.1"
                     /translation="MTDGAHPRVSRRIGSIAESATLAVDARAKALKAAGRPIIGFGAG
                     EPDFATPAAIVEAAVEAARDPKNHRYTPAAGLPELREAIAVKTARDSGFKVTASEVLV
                     TNGGKQAVYQAFATVLDPGDEVILPAPFWTTYPEAIALAGGVPVQVDTDESSGFLVSV
                     AQLEAARTPRTKALLFCSPSNPTGAVYSPDQVAEIGRWAVEHGIWVLTDEIYEHLVYG
                     DATFSSMPVVVPELRSTCIVLNGVAKTYAMTGWRVGWMIGPSDVIKAATNLQSHLTSN
                     VANVSQRAALAAVSGDLSAVAMMREAFDRRRQMMVTMLNEISGVVCVEPKGAFYAYPS
                     VKALLGLELRGRRPATSAGLAELVLEEAEVAVVPGEAFGTPGYFRLSYALGDDDLVEG
                     VNRLGKLLSEAR"
     gene            complement(1403..>1704)
                     /locus_tag="VGD55_08310"
     CDS             complement(1403..>1704)
                     /locus_tag="VGD55_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEY0870387.1"
                     /translation="FFTNPVLREADVPAGAPRWPGADGRVKTSAAWLIEQAGFHRGFG
                     DGRVGLSAKHTLALVNRGGATTTELIAFARQIRDGVAQRFGVTLDVEPVLVGVRL"
BASE COUNT          280 a          590 c          552 g          282 t
ORIGIN      
        1 gaacccccaa ccgccggttt tggagaccgg aactctggcc agttgagcta ctgcccttcg
       61 tggcaccccg cgaaccgtta ccgccctgta ccgctggggt ccagcctcga cagtgtacgt
      121 gccacggctg gaacggtgaa acccggaggc gttcgcgcgc gcccgcgtgg gacgatgaat
      181 ctcatgacag acggagcgca ccctcgggta tctcgccgca tcggatccat cgcagagtcg
      241 gcgaccctcg ctgtcgatgc gcgcgcgaaa gcgttgaagg ctgccggtcg gccgatcatc
      301 ggcttcggcg caggcgagcc cgactttgcg accccggcgg caatagtcga ggccgccgtc
      361 gaagccgccc gcgacccgaa gaaccatcgc tacacccctg ccgccggcct gcccgagttg
      421 cgcgaagcca tcgcggtcaa gaccgcccgc gactcaggat tcaaggtcac cgcatcggag
      481 gtcctcgtca ccaacggcgg gaaacaggcg gtctaccaag ccttcgcaac agtgctcgat
      541 cccggtgacg aagtaattct gccggcacct ttttggacca cctaccccga ggcgatcgcg
      601 ctcgctggcg gcgtccccgt gcaggtcgac accgatgagt cgagtggatt cctggtcagt
      661 gtcgcgcagc tcgaggcagc ccgcaccccg cgcacgaagg cgctgctgtt ctgctccccg
      721 tcgaacccga ctggcgcggt ctactcaccc gaccaggtcg ccgagatcgg ccgctgggcg
      781 gtcgagcacg gcatctgggt gctcaccgac gagatctacg agcacctcgt ctacggcgac
      841 gcgacgttct cgtcgatgcc cgtcgtcgtc cccgaacttc gcagtacctg catcgtcctc
      901 aacggtgttg cgaagaccta cgccatgacc gggtggcggg tgggctggat gatcggcccg
      961 tccgacgtca tcaaggccgc caccaacctt cagtcgcacc tgacctcgaa cgtcgccaac
     1021 gtctcgcagc gggccgcgct cgcggccgtg tcgggcgacc tctccgctgt cgcgatgatg
     1081 cgcgaagcgt tcgaccggag gcgccagatg atggtgacga tgctgaacga gatttccggc
     1141 gttgtgtgcg tcgagccgaa gggcgcgttt tacgcctatc cgtcggtcaa ggcgctcctg
     1201 ggtctcgagc tgcggggccg gcgtccggcg acgtcggctg ggctggccga gcttgtgctc
     1261 gaagaagccg aggttgcggt cgtcccgggt gaggcgttcg gtacccccgg gtacttccgg
     1321 ctctcgtacg cgcttggcga cgacgacctc gtcgagggcg tgaaccggct cggcaagttg
     1381 ctgtccgaag ctcgctaacg ctctacagcc ggacgccgac cagcaccggt tcgacgtcga
     1441 gcgtcacacc aaagcgctgg gcgacaccat ctcggatctg ccttgcgaac gcgatcaact
     1501 cggtggtggt cgcgccgcca cggttgacca gcgcgagggt gtgtttggcc gacaacccga
     1561 cgcggccgtc gccaaatccc cggtggaatc cggcctgctc gatcagccat gccgccgagg
     1621 tcttcacccg gccgtcagca ccgggccagc gcggcgcacc agccggaacg tcggcttcgc
     1681 gcagcacggg attggtgaag aaag
//
LOCUS       DASWVF010000465         8872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_140045, whole genome shotgun sequence.
ACCESSION   DASWVF010000465 DASWVF010000000
VERSION     DASWVF010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140045"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGD55_08315"
     CDS             complement(<1..327)
                     /locus_tag="VGD55_08315"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEY0870388.1"
                     /translation="MATAGPHDPGWYADPLGAGGMRWWNGATWAGPASVPLAPGRRLA
                     GWAKTCLAVGIAIALANLALVSLMLFAVVFFVGGARTVSTTGDLWVCAAVGLAVASLA
                     AAARGAA"
     gene            512..1543
                     /gene="argC"
                     /locus_tag="VGD55_08320"
     CDS             512..1543
                     /gene="argC"
                     /locus_tag="VGD55_08320"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0870389.1"
                     /translation="MGLSAAVAGASGYAGGELLRLLDAHPDITADQIMAASNAGEPVT
                     SVHPHLRQYTGRVFEQTDPARLAEADVVFLALPHGQSAAIASQLPEHTVIVDLGADFR
                     LRDASQWMTYYGGTHAGAWTYGLPELPGARALIASSKRIASPGCYATAITLALAPLYT
                     AGLAEPDDVVVVAASGTTGAGRAMKQHLMASDVMGSLLPYKVGGTHQHTPEIEQALSD
                     VAGAPVSISFTPILAPMPRGILATCTARPKPGVTASDLRAAFKAAYLSEHFVHVLPDG
                     EWPQTGATLGSNATHLQVALDEHAGRIVVVSALDNLTKGAAGQAIQNANIALGLDEAT
                     GLPTIGVTP"
     gene            1540..2694
                     /gene="argJ"
                     /locus_tag="VGD55_08325"
     CDS             1540..2694
                     /gene="argJ"
                     /locus_tag="VGD55_08325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY0870390.1"
                     /translation="MSVTAAKGFRAAGVIAGIKPSGRSDVALVVNDGPIRAAAGVFTG
                     NRIKAAPVKWSQQVLKGGKVAAVILNAGNANACTGSVGFQHTHRTAEEVAALLDYSAA
                     EVAVCSTGLIGEYLPIEKLLTGVHAAYDALSTGGGDDAARAIMTTDTVAKQTVISSPQ
                     GWTVGGMAKGAAMLAPGLATMLVVLTTDAVADAATLDRSLRTGTAKTFDRLDSDGCMS
                     TNDTVLLLASGASGVEADVDELAGAVTQACASLAAQLVDDCEGASKVIEIEVVGAVSE
                     SDAVDVARAVSRSNLLKCAIHGQDPNWGRILAALGTTSATYDDDGVDVAINGVWICRG
                     STIGADRTLVDMSARRVEITIDLHAGDAAATLLTTDLTAEYVHENSAYST"
     gene            2708..3622
                     /gene="argB"
                     /locus_tag="VGD55_08330"
     CDS             2708..3622
                     /gene="argB"
                     /locus_tag="VGD55_08330"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0870391.1"
                     /translation="MSRDLELAQAKAATLHEALPYLARFHGSTVVVKYGGHAMTDPVL
                     AASFAADIVFLRYAGLRPVVVHGGGPQINAQLDTLGIEPKFAGGLRVTTAQTMNVVRM
                     VLVGQVQRDVVGLLNAHGPFGVGLSGEDGQLFTAQRRPAIVDGEPVDIGFVGDISAVE
                     PGVVNSLLDDGRIPVVSSVARGFDDDGTPCIYNVNADTAAGALAVALGAEKLVVLTDV
                     EGLYADWSPDGSSNSVISSLSCDELEVLLPTLSAGMVPKMEACLRAVRGGVPSAHVLD
                     GRVPHAVLLEIFTDEGIGTMVRAKAYDE"
     gene            3615..4805
                     /locus_tag="VGD55_08335"
     CDS             3615..4805
                     /locus_tag="VGD55_08335"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEY0870392.1"
                     /translation="MSNADLLARFNAVMMPTYSPSVPLVRGEGCTVWDADGRAYLDLI
                     AGIAVSSLGHAHPAVVDAVSKQIATIAHTSNLFCNEPALLLAERLLGLLGGSGRVFFT
                     NSGTEANEAAFKLVRKFAGPDRTYVVAAEKGFHGRTMGSLALTGKPSIREPFGPYGAE
                     VVFVPYGDVEALTAAVTGKTAALFLEPMLGEGGVVPAPAGYLRAAREICTATGAVFVL
                     DEIQSGIGRTGSWFLHHSDGVVPDVITLAKGIGGGLPIGVCIGIGTFGEVFGKGDHGS
                     TFGGNPVASAAALAVIDTIENDDLLGHVAKVGHHFAQGLHAVGHPALAGVRSRGLWLA
                     LALSDPNAPAVEKAAREAGFLVNAVQPDAIRIAPPLVLSLDEADRFVAALPAILDAAT
                     GGEL"
     gene            4802..5725
                     /gene="argF"
                     /locus_tag="VGD55_08340"
     CDS             4802..5725
                     /gene="argF"
                     /locus_tag="VGD55_08340"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0870393.1"
                     /translation="MTARHFLIDDDLQPFEVRAILDQADHLKKDRLALRPLAGPRSVA
                     VLFEKQSTRTRVSFEVGIAELGGHPLIIDARTSQLGRGEPIEDTGRVLSRYVSAIVLR
                     TYGHERIEALAASASVPVVNALSDLVHPCQWLADLMTIREHRGEPAGQTITYVGDGNN
                     VAHSILLGGVLAGMHVRVAAPVGYEPLPEIVERAEAIAVHTGGSVAVTDDPIAAATDV
                     DVLYTDVWASMGQEEESATRQLAFAPYQLDDRLVALAADDVMVLHCLPAHRGEEISAS
                     VLDGPHSFVFDQAENRLHAQKALLAFLLDQS"
     gene            5722..6279
                     /locus_tag="VGD55_08345"
     CDS             5722..6279
                     /locus_tag="VGD55_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEY0870394.1"
                     /translation="MSGRQHSTVHVTARTDAPGTKAARHARIIDLLAWTPVHSQSELV
                     GLLAADGFSVTQATLSRDLDEIGAVRLRGAGGGFVYVVPRDGDDAVSRPTSDRVHNAS
                     AAHARLIRMAGELLVSVRASGNIVVARTPPGGAHLLAGAIDHADLATVIGTVAGDDTV
                     LCVCRDPGGGDDVAEQLLHLSDRKT"
     gene            6306..7523
                     /locus_tag="VGD55_08350"
     CDS             6306..7523
                     /locus_tag="VGD55_08350"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0870395.1"
                     /translation="MTERVVLAYSGGLDTSVCIGWIAAETGAEVIAVAADVGQGGEDM
                     DVIRARALACGAVEAIVVDAKDEFADEYCLPALKANALYMDRYPLVSALSRPVINKHL
                     VEAAHRFGATRVAHGSTGKGNDQVRFEVGIGALDPNLTVLAPVRDSGMTRDKAIAFAE
                     EKGLPIDVNKKSPYSIDANVWGRAIETGFLEDIWNAPIEDIYAYTANPASPRAADEVV
                     ITFDQGKPVAIDGKPVTVLRAISELNTRAGAQGVGRLDMVEDRLVGIKSREVYEAPGA
                     IALITAHQELENVTTERDLARFKRQVEQRWGELVYDGLWFSPLKYALDEFIAFSQRYV
                     TGDVRLTLHGGRAVVTGRRSPASLYDYKLATYDTGDVFDQSLAKGFVELWGLPSKTAA
                     RRDLAVQAKTEKS"
     gene            7527..>8872
                     /gene="argH"
                     /locus_tag="VGD55_08355"
     CDS             7527..>8872
                     /gene="argH"
                     /locus_tag="VGD55_08355"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0870396.1"
                     /translation="MSDSGPLDVPSGEPLRLWGGRFEGGPAAALAALSVSVHFDWRLA
                     TYDLLSSRAHARVLHRAGLLDDAELATMLAALDDLDAACQSGAFVPTVADEDVHTALE
                     RGLIERVGALGGKLRAGRSRNDQIATDLRLYLRAQVRVLVERVTELESALVDVAERHK
                     LAPAPGMTHMQHAQPILVAHHLLAHVHAFSRDVDRLRDWDARAAVSPLGAGALAGSSL
                     PLDPVTTARELGFTSSAANSIDAVSDRDFAAEFAFCAAMIGIHLSRIGEEIVLWTSRE
                     FRWVELDDSYATGSSIMPQKKNPDVAELARGKTGRLVGNLTAILTMLKGLPLSYDRDL
                     QEDKEPVFDSLDTLLVLLPAVTGMIATMRIDTARLAAAAPEGFALATDIAEDLVRRGV
                     PFREAHEAVGRLVAWCSANDFEMQDVDDAQLAALSPHLTPQIRLVLTVEGALAARSS"
BASE COUNT         1465 a         2945 c         2960 g         1502 t
ORIGIN      
        1 agccgcgcct cgcgctgctg cggccaagct cgcaacggca aggcccacgg ctgcgcatac
       61 ccacagatcc ccagtcgtgc tgaccgttcg agcaccacca acgaaaaaca ccacggcgaa
      121 tagcatcagc gagacgagcg ctaggttcgc gagagctatc gcgatgccga ccgcaaggca
      181 tgtcttcgcc caccccgcca gccgccgtcc gggcgccagc ggaactgacg ccggacctgc
      241 ccatgtcgca ccgttccacc accgcatgcc gcccgcaccc agggggtctg cgtaccagcc
      301 tgggtcgtgc gggcctgccg tcgccatgcc tagaccatcg gtggctgccc ggtgcgagtt
      361 gaaacgcgat cggtaggccc ggcgaatgtg ggccattctg ttcgcgcgag cgaccggaac
      421 ggtccacccg aacgactacc gtacgagtgg tgtatgctta tgcgggtaga tgcataaaca
      481 tcgtgcgagt ggacggcgag gggcgggcga gatgggactc agtgcggctg tagcgggcgc
      541 cagcggctac gccggtggcg agctgctgcg gctgctcgac gcccatccgg acataaccgc
      601 cgaccagatc atggccgcct cgaacgcggg ggaaccggtg acatcggtgc acccgcacct
      661 gcggcagtac accgggcggg tgttcgagca gaccgatccg gcgcggctcg ccgaggccga
      721 cgtcgtgttc ctcgcgttgc cccacggcca gtcggcggcg atcgcgtcgc agttacccga
      781 gcacaccgtc atcgtcgatc tcggcgccga tttccggctg cgcgacgctt cgcaatggat
      841 gacgtactac ggcggcaccc acgcgggcgc gtggacctac ggcctgccag aactgccagg
      901 tgcccgagcc ttgatcgcca gcagcaaacg gatcgcgagc ccgggctgct acgcaacagc
      961 catcacgctg gcactcgcgc cgctctacac cgcagggctc gcagaacctg acgacgttgt
     1021 cgtggtggcg gccagcggta cgaccggcgc gggccgtgcg atgaagcagc acctcatggc
     1081 gagtgacgtc atggggtcgc tgttgccgta caaggtcggc ggcacccatc agcacacccc
     1141 cgagatcgaa caagctttat cggacgtcgc gggcgctccc gtgtcgatct cgttcacgcc
     1201 gattctcgcg ccgatgccac gcggcatcct ggcgacctgc acagcgcggc cgaagcccgg
     1261 cgttaccgca agcgacctgc gggccgcctt caaagcggcc tatctgagcg aacacttcgt
     1321 gcacgtgctg cccgacggcg agtggccgca gaccggcgcg acactcggct cgaacgcgac
     1381 ccacctccag gtcgctctcg acgagcatgc cggccgcatc gtcgtggtgt cggcgctcga
     1441 caacctcacg aaaggcgctg ctggccaggc gattcagaac gcgaacatcg cgctcgggct
     1501 cgatgaggcg actgggcttc ccaccatcgg agtgaccccg tgagcgtcac cgcagccaag
     1561 gggttccggg ccgccggcgt gattgccggc atcaagccga gcgggcgcag cgacgtcgcg
     1621 ctcgtcgtca acgatgggcc gatccgcgct gcggctggtg tgttcaccgg taaccgcatc
     1681 aaagccgctc cggtgaagtg gtcgcagcaa gtgctgaagg gcggcaaggt cgcggccgtg
     1741 atcctcaatg caggcaacgc gaacgcgtgc actggcagcg tcgggttcca acacacccac
     1801 cgcaccgccg aggaggtcgc tgcgctgctc gactattcgg ccgccgaggt ggcggtgtgt
     1861 tccaccggcc tgatcggtga gtacctgccg atcgaaaagc tgctgaccgg cgtccatgct
     1921 gcgtacgacg cactttcaac tgggggtggc gacgatgccg ccagggccat catgacgacc
     1981 gacaccgtgg ccaagcagac ggtcatctcg tctcctcaag gttggaccgt cggaggcatg
     2041 gcgaagggtg ccgcgatgct cgcgcctggc cttgccacga tgcttgtggt gctgacaact
     2101 gacgccgttg ccgatgccgc aaccctcgac cgctcgctgc gtacggggac cgcgaagacc
     2161 ttcgaccggc tcgattccga cggttgcatg tcgacgaacg acactgtgct actgctcgcg
     2221 tcgggagcgt ccggcgtcga ggccgacgtc gacgaacttg cgggtgcggt cacgcaggcg
     2281 tgtgcctcgc tcgcggccca actcgtcgat gactgcgaag gtgcaagcaa ggtcatcgag
     2341 atcgaggtcg tcggcgcggt gagcgaatcc gatgccgtcg atgtcgcgcg cgcggtctcg
     2401 cgcagcaacc tcttgaagtg cgcgattcac ggccaggatc ccaactgggg tcgcattctc
     2461 gccgcgctcg ggacgacatc ggcgacctac gacgacgacg gcgtcgacgt tgctatcaac
     2521 ggcgtctgga tctgccgtgg ctcgacgatc ggcgccgacc ggacgctggt cgacatgtca
     2581 gcccggcggg tcgagatcac gatcgatctg cacgcgggcg atgcggccgc aacgctgctg
     2641 acgactgacc tcaccgccga gtacgtgcac gagaactcgg cgtactccac atgagaaaca
     2701 tccgaacatg agcagggatc tcgaattggc gcaggcgaag gccgccacgt tgcacgaggc
     2761 gttgccctac ctggcgcgct tccacggcag cacggtcgtt gtgaagtacg gcggtcatgc
     2821 gatgaccgac ccggtgcttg cggcgtcgtt cgcggccgac atcgtgttct tgcgttatgc
     2881 cggcttgcgc ccggtcgtcg tgcatggtgg cggtccgcag atcaacgccc agctcgatac
     2941 gttgggcatc gagccgaagt tcgcgggcgg cctgcgcgtg acgaccgcgc agacgatgaa
     3001 cgtcgtgcgc atggtgctcg tcgggcaggt gcagcgcgac gttgtcggtc tgctcaacgc
     3061 ccacggcccg tttggcgtcg gtctaagcgg tgaagacggc cagcttttca ccgcacagcg
     3121 gcgcccggcc atcgtcgacg gcgagccggt cgacatcggg ttcgtgggag acatcagcgc
     3181 cgtcgagccg ggcgtcgtca acagcctgct cgacgacggc cgcatccccg tggtttccag
     3241 cgtggcaagg ggtttcgacg acgacggcac cccttgcatt tacaacgtca acgccgacac
     3301 ggcagcaggg gccctcgcgg tggcgctcgg tgccgagaag ctcgtcgtgc tcaccgacgt
     3361 cgagggactt tatgccgact ggtcgcctga cggttcaagc aatagcgtga tcagctcgtt
     3421 gagctgtgac gaactcgagg ttttgctgcc cacgctctcg gcgggcatgg tgccgaagat
     3481 ggaagcgtgc ctgcgtgccg ttcgtggagg ggtgccgagc gcccacgttc tggacggacg
     3541 cgtaccccac gccgtcctgc tcgaaatctt taccgatgaa gggatcggca cgatggtccg
     3601 cgcgaaggcc tacgatgagt aacgccgacc tgctggcgcg attcaacgcg gtgatgatgc
     3661 cgacgtattc gccgtccgtt ccgctcgtgc gcggcgaggg ttgcaccgta tgggacgccg
     3721 acgggcgcgc gtacctcgac ctgatcgccg gcattgcggt gtcgtcttta gggcacgcac
     3781 atccggccgt ggtcgacgca gtgtcgaagc agatcgccac gatcgctcac accagcaacc
     3841 tcttttgcaa cgagcctgcg ctcttgctgg cggaacggct gctcggattg ctcggaggca
     3901 gcggtcgggt gttcttcacc aacagcggca ctgaggccaa cgaggccgcg ttcaagctgg
     3961 tgcgcaagtt cgctgggccc gatcgcacct atgtcgtcgc ggcggagaag ggctttcacg
     4021 gccgcacgat gggatccctt gccctgacgg ggaaaccgtc gattcgcgag ccgttcgggc
     4081 cctacggtgc cgaggtcgtt ttcgttccct acggtgacgt ggaagctttg acggcagcgg
     4141 tcaccggcaa gaccgccgcg ctgttcctcg agccgatgct cggcgagggc ggcgtcgtac
     4201 ctgcgcccgc tggctacctg cgcgccgctc gcgagatctg cactgcgacc ggcgcggtgt
     4261 tcgtgcttga tgagatccaa tcgggcatcg gccgtactgg ttcatggttc ctgcaccaca
     4321 gcgatggggt cgtgcccgac gtcatcaccc ttgcgaaggg gatcgggggc ggcttgccga
     4381 tcggcgtctg tatcggcatc ggcacgttcg gtgaggtgtt cgggaagggt gatcacggca
     4441 gcacgttcgg tggcaacccg gtcgcgagcg cggccgcgct cgcggtgatc gacaccatcg
     4501 agaacgacga cctgctcggc catgtcgcca aggtcggtca ccatttcgcc cagggcctgc
     4561 acgcagtggg gcacccggca ctcgccggcg tccgcagtcg tggcttgtgg cttgcccttg
     4621 ccttgagcga cccgaatgcg ccggccgtcg agaaagcagc ccgcgaagcg ggtttccttg
     4681 tcaacgccgt acagcccgac gcgatcagga tcgcgccgcc actcgtgctg tcactcgacg
     4741 aggccgaccg gttcgtcgcc gccctgcccg cgatcctcga cgcggcgacg ggcggcgagc
     4801 tatgacggcc cgtcacttcc tgattgacga cgatctccag ccgttcgagg tgcgcgcgat
     4861 cctcgaccag gccgatcacc tgaagaagga ccgactggcg ctacggccac tcgcgggccc
     4921 gcggtcggtc gcggtgctgt tcgaaaagca gtcgacccgc actcgggtct cgttcgaggt
     4981 cggcattgcc gaactgggcg gacatccgct gatcatcgat gcgcgcacat cgcaactcgg
     5041 ccgcggcgag ccgatcgaag acaccgggcg ggtgctgtcg cgctacgtat ccgcgatcgt
     5101 gttgcgcacg tacgggcacg agcggatcga ggctctagcg gcgagtgcga gcgtgccggt
     5161 cgtgaatgcg ctctcggatc tggtccatcc ctgccagtgg ctcgccgatc taatgaccat
     5221 tcgcgagcat cggggcgaac ccgctggcca gacgatcact tacgtcggcg acggcaacaa
     5281 cgttgcgcac tcgatcctgc tgggcggggt gctcgccggc atgcacgtgc gggtcgcagc
     5341 cccggtgggc tacgaaccgc tgcctgaaat cgtcgagcgg gccgaagcga ttgcggtaca
     5401 taccggtggc agtgtcgccg tcaccgacga tccgatcgct gcagcgactg atgtcgatgt
     5461 cctgtacacc gacgtctggg cctcgatggg gcaagaggaa gagtcggcaa cgcggcaact
     5521 cgccttcgcg ccctaccagc tcgacgatcg tctcgtcgcg ttggctgccg acgatgtcat
     5581 ggtgctgcat tgcctgcctg cgcatcgagg cgaggagatc agtgcgtcgg tactcgacgg
     5641 tccgcactcc ttcgtcttcg atcaggcaga aaaccgcctg cacgcccaaa aggcactcct
     5701 cgcattcctg ctggaccaat catgagtggg cgccagcaca gcaccgtcca cgtaacagct
     5761 cgtactgatg cgcccgggac caaggcggcc cgccacgccc ggatcatcga ccttctcgca
     5821 tggacgccgg tgcactcgca atcggagctc gtcggtctgc ttgctgccga cgggttctct
     5881 gtcacgcagg cgacgctgtc gcgcgacctc gacgagatcg gcgccgtgcg gctgcgcggg
     5941 gccggaggtg ggttcgtgta cgtggtgcca cgcgacggcg acgatgccgt cagcaggccg
     6001 acgtccgacc gggtgcacaa cgcctcggcc gcacacgccc ggctgatccg catggcgggg
     6061 gagttgctcg tctcggtgcg agcgtcgggc aacatcgtgg tggcccgcac accgcccgga
     6121 ggtgcccact tgctggccgg cgccatcgac catgctgacc tcgctaccgt catcggcacg
     6181 gtggcgggtg acgacaccgt gctctgcgtg tgccgtgacc ccggcggggg cgacgacgta
     6241 gccgagcaac tgctgcacct ttccgatcgc aagacctaaa cccgccaaac tcagggagaa
     6301 gtttcgtgac cgagcgcgtc gtactcgcat attcgggagg ccttgacacc tccgtgtgca
     6361 tcggctggat cgccgccgag accggtgcgg aggtcatcgc ggtcgccgcc gacgtcgggc
     6421 agggcggcga agacatggac gtgatccgcg cgcgagcgct tgcctgcggt gccgtcgaag
     6481 cgatcgtcgt cgacgcgaag gacgagttcg ccgacgagta ctgcctaccg gcgctcaagg
     6541 cgaacgccct ctacatggat cgctacccgc tggtctccgc cctgtcgcgc cctgtcatca
     6601 acaagcacct cgtcgaggct gcccacaggt tcggcgctac cagggtcgcg cacggtagta
     6661 ccggcaaggg caatgaccag gtgcgtttcg aggtcggcat cggtgcgctc gatccgaatc
     6721 tcacggtgct cgcccccgtg cgtgactcgg gcatgacccg cgacaaggcc atcgcgttcg
     6781 cggaggaaaa gggtctaccg atcgacgtca acaagaagtc gccgtattcg attgacgcga
     6841 acgtgtgggg tcgtgcgatc gagacaggct tcctcgagga catctggaac gcgccgatcg
     6901 aggacatcta cgcctacacc gcaaaccccg cgagcccgcg tgcggccgac gaggtcgtca
     6961 tcacgttcga ccagggcaag ccggttgcca tcgacggcaa gccggtgacc gtcttgcggg
     7021 cgatcagcga gttgaacacc cgggcaggcg ctcagggtgt cggccggctc gacatggtcg
     7081 aagaccggct cgtcggtatc aagagccgcg aggtctacga agcccccggc gcgatcgcgc
     7141 tgatcactgc gcaccaggag ctcgagaacg tgacgaccga acgcgatctc gcgcgtttca
     7201 agcggcaggt cgaacaacgc tggggtgaac tcgtctacga cggcctatgg ttctcgccgc
     7261 tgaagtacgc gctcgatgag ttcatcgcgt tctcccagcg ttatgtcacc ggcgacgtcc
     7321 ggctgaccct gcacggcggt cgtgcagtcg tgaccggacg tcgcagcccg gcgtccctct
     7381 acgactacaa gctcgcgacc tacgacacgg gtgacgtgtt cgaccagtcg ctggcgaagg
     7441 gcttcgtcga gctctggggg ctgccgagca agaccgcggc ccggcgcgac ctcgcggttc
     7501 aggccaagac cgagaaatcc tgacggatga gcgattctgg gccactcgac gtcccgtcag
     7561 gtgagccgtt gcgactgtgg ggtggccgct tcgaaggtgg tccggcggcc gcgctcgctg
     7621 cattgtcggt ctcggtgcac ttcgactggc ggctcgcgac ctacgacctg ctcagttcgc
     7681 gcgcgcacgc ccgggtgttg catcgggccg ggctgctcga cgacgccgag ctcgcaacga
     7741 tgctcgccgc gctcgacgat ctggatgccg cctgccaatc gggggcgttt gttccgaccg
     7801 tcgccgacga ggacgtgcac acggctctcg aacgcggact catcgaaagg gtcggcgccc
     7861 ttggcggcaa gttgcgggcg ggtcgcagcc gcaacgacca gatcgcgacc gatctccggc
     7921 tctacctgcg ggcgcaggtg cgggtgctcg tcgagcgcgt gaccgagctc gaatcggcgc
     7981 tcgtcgatgt cgccgagcga cacaagctgg cgcccgcgcc gggcatgaca cacatgcagc
     8041 atgcccagcc catcttggtt gcccaccacc tactcgcgca cgttcatgcg ttctcgcggg
     8101 acgtcgaccg gttgcgcgat tgggacgcgc gcgccgccgt ttcccctttg ggcgccggcg
     8161 ctctcgccgg ttcttcgttg cccctcgacc cggtcacgac ggcgagagag ctcggcttca
     8221 cgtcgtccgc cgcgaattcg atcgacgccg taagtgaccg cgacttcgcc gccgaattcg
     8281 cattctgcgc ggccatgatc ggcattcacc tgtcgcgaat cggcgaagag atcgtgctct
     8341 ggaccagcag agaattccgg tgggtggagc tcgacgacag ttacgcgacc gggtcgtcga
     8401 tcatgccgca gaagaagaat ccggacgtcg cggagcttgc gcgcgggaag actgggcgtc
     8461 tcgtcggcaa cctgacggcg atcctcacga tgctgaaggg tttgccgctg tcgtacgacc
     8521 gcgatctgca ggaagacaag gagccggtat tcgactcact cgacacactc ctcgtgctgc
     8581 tgcccgcggt taccggcatg atcgcgacga tgcgcatcga taccgcgcgc ctggccgctg
     8641 ccgcaccaga agggttcgcg ctagcgaccg acatcgctga ggaccttgtc cgtcgcggcg
     8701 ttccgttccg ggaggcgcac gaggcggtcg gccgcctcgt cgcttggtgt tcggcgaatg
     8761 attttgagat gcaggacgtc gacgacgctc aactcgcggc cctatcgccg cacctgacac
     8821 cgcagatccg gttggtgctg accgtcgagg gcgcgttggc ggctcgttcg tc
//
LOCUS       DASWVF010000466         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_292685, whole genome shotgun sequence.
ACCESSION   DASWVF010000466 DASWVF010000000
VERSION     DASWVF010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292685"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..870)
                     /locus_tag="VGD55_08360"
     CDS             complement(49..870)
                     /locus_tag="VGD55_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY0870397.1"
                     /translation="MAEITTLADAVATHVHDGDTLAMEGFTHLIPVAAGHEIIRQGRR
                     NLTLVRMTPDIIYDQLIGAGCARKLVFSWGGNPGVGSLHRFRDAVESAWPAPLEIEEH
                     SHAGMANRYVAGASGLPFAVLRGYVGTDLAAQTPSIKTVTCPFTGEVLTAVPALNPDV
                     TIVHAQRADRAGNVQMWGIVGVQKEAVLAARRVIVTVEEIVDVLEPRPGAVLLPSWVI
                     TAVAQVQHGAHPSYAQDYYDRDNAYYQAWDEISRDRESFTHWVDEHVLVSQRGAS"
     gene            complement(873..2069)
                     /locus_tag="VGD55_08365"
     CDS             complement(873..2069)
                     /locus_tag="VGD55_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY0870398.1"
                     /translation="MTRTQVAIVGAGPAGLTLAHLLHLQGIDSVVVEARGRDYVEHRV
                     RAGVLEQPTVDLLHEVGLGYRVAREGLEHHGTTIRFNRRSHRIDFTELTGSSITVYGQ
                     QEVVKDLIAARIENDLPLLFEVTDARIDDATTTRPRVRYVHDGNEQVIEADIVAGCDG
                     FHGISRPTVPTDVLTIFERTYPFAWLGILARTHPSSEELIYANHENGFALHSMRSPTV
                     TRQYLQVAPDEDLENWSDARIWAELKTRFDDEEGFDLEEGEIFEKGITPMRSFVAEPM
                     QHGRLFLVGDAAHIVPPTGAKGMNLAIADACVLSHAVGAFYATGSEVLLDAYTATCLR
                     RVWRAEHFSWFMTSMLHRPPDQDAFDRRLQLSQLDYVTSSRAAATSLAENYVGLPFPT
                     PWHWKD"
BASE COUNT          363 a          722 c          741 g          406 t
ORIGIN      
        1 tcatcatctc gtcggacgtc caggcaggtg ccgtcacggc tggttctgtc acgacgcacc
       61 tcgctgcgac acgagtacgt gctcgtcgac ccagtgcgtg aacgactcgc ggtcgcgact
      121 gatctcgtcc cacgcctggt agtaggcgtt gtcgcggtcg tagtagtcct gcgcatacga
      181 cgggtgcgcg ccgtgttgca cctgcgcgac cgcggtgatc acccatgacg gaagcagcac
      241 cgcacccggc cgcggctcga gcacgtcgac gatctcctcg actgtcacga tcacccggcg
      301 tgctgcaaga actgcttctt tctggacgcc gacgatgccc cacatctgca cattgccggc
      361 ccggtcggcc cgttgggcat gcacgatcgt cacgtcggga ttcaacgcag gcactgcagt
      421 gagcacctcg ccagtgaacg ggcacgtcac ggtcttgatc gagggagttt gcgccgcgag
      481 gtcggtgccg acgtaaccgc gcaacaccgc gaacggcagg cccgatgcgc cggcgacata
      541 gcggttcgcc atgcccgcat ggctgtgttc ttctatctcc agaggcgccg gccaggccga
      601 ctcgaccgcg tcgcggaacc ggtgcagtga cccaaccccg gggttgccgc cccaggagaa
      661 caccagcttg cgggcgcagc cggccccgat gagctggtcg tagatgatgt cgggcgtcat
      721 gcgcacgagc gtcaagtttc ggcgtccctg cctgatgatt tcgtgccctg ccgccaccgg
      781 aatgaggtgg gtgaaaccct ccatcgcaag ggtgtcgccg tcgtggacgt gggtggccac
      841 tgcgtcggca agggtcgtga tctcagccat cgtcagtcct tccagtgcca cggcgtcggg
      901 aagggcaggc cgacgtagtt ctccgccaac gacgtggccg ccgcgcgtga cgacgtgacg
      961 taatcgagct gcgacaactg aagtcggcga tcgaacgcgt cttggtctgg cgggcggtgc
     1021 agcatcgagg tcatgaacca cgagaagtgt tcggctcgcc acactcggcg caagcaggtt
     1081 gcggtgtagg catcgagcag cacctcgcta ccggtcgcgt agaaggcgcc gacggcgtga
     1141 ctgagcacgc acgcgtcggc gatggcgagg ttcatgccct tggcacctgt tggaggcacg
     1201 atatgcgctg cgtcgccgac caggaacagc cgtccgtgct gcatcggctc ggcgacaaag
     1261 ctgcgcatcg gggtgatgcc cttctcgaag atctcaccct cttcgaggtc gaacccctcc
     1321 tcgtcgtcga accgggtctt caactcagcc caaatgcgcg cgtcggacca gttctcgagg
     1381 tcttcgtcgg gcgcgacttg gaggtactgc cgggtcacgg tgggcgaccg catgctgtgc
     1441 agcgcgaagc cattctcgtg attcgcgtaa atcaattcct cggacgacgg gtgcgtgcgt
     1501 gccagtatcc ccagccacgc gaacgggtac gtccgctcga aaatagtgag cacgtcggtg
     1561 ggtacggtcg gcctgctgat gccgtgaaac ccgtcgcagc ccgcgacgat gtcggcctcg
     1621 atgacctgct cgtttccgtc gtgcacgtac ctcacgcgcg ggcgcgtcgt cgtcgcgtcg
     1681 tcgatgcgtg cgtcggtgac ctcgaagagc aacggcaggt cattttctat gcgcgcggcg
     1741 atgaggtctt tcacgacctc ctgctggccg tacaccgtga tcgacgaacc ggtcagttcg
     1801 gtgaagtcga tgcggtgcga gcggcggttg aagcggatcg tcgtgccatg gtgctcgagt
     1861 ccttcgcggg ctactcggta cccgagcccg acttcgtgta gcaggtcgac cgtcggttgc
     1921 tcgagtacgc cagcccgcac gcggtgctcg acgtagtcgc ggccgcgcgc ctcgactacc
     1981 accgagtcga tgccttgcag atgcagtagg tgggcgagcg tcaatcccgc cgggcctgca
     2041 ccgacaatgg cgacctgagt tcgcgtcatg ggtagaaccg gaagatgggt tgggcgacgc
     2101 acaccggctt gccgctaccc tcgcgctcga tcaccgcgtc gacggtcagc tggacgccgc
     2161 cctcgatgtc gtcgacggcg gccagggtgg ccgtggtccg gattctcgag cccgccggca
     2221 cgggggctgg ga
//
LOCUS       DASWVF010000467         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_471331, whole genome shotgun sequence.
ACCESSION   DASWVF010000467 DASWVF010000000
VERSION     DASWVF010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471331"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..>1592
                     /locus_tag="VGD55_08370"
     CDS             222..>1592
                     /locus_tag="VGD55_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0870399.1"
                     /translation="MTEIAEERPADRHPIDPSTFDVPVVVLSHRAPVAFYEENGHRLA
                     RRGAGGLVTALVGLAAQLNDSVWVCAASTDEDIAVAREYKDAPLHVAMTTHSSIPPIT
                     TADAEGSLQIRFVEVDPVAHEDFYGVISNPLLWFVQHGLYDLSYSPNIGQRERDAFED
                     GYVSVNETFADAVVHEVERCGRRALVMLHDYHFYLVADLVRKRCPDVVLSHFVHIPWP
                     GPDAWRVLPPDIRERLFYGLLGNDVVAFHTERFARNFLLCAQELLGLTIDLDRMTVEI
                     GPRTVTARTYPISIDVAALVETAGSKAVRNRLDDLEQWLVADDAKLIVRVDRTDPSKN
                     IIRGFLAFEKLLCDHPELVGKVRFLAFLQPSRLDVPEYAQYVADIGSVVARVNARYTS
                     RGEAPIDLRLHEDFPTAVAAYTICDVVMVNSLADGMNLVAKEAVSVSRRNAVLALSEN
                     TGAHEEL"
BASE COUNT          273 a          546 c          498 g          275 t
ORIGIN      
        1 ctcttcttct gtcttcgcgc ggcgacacca ccccggccag tcgagcgaac aggcaaaaac
       61 ccgcgtggtg cccagctcga ggtagaccgg ttcgcgcggc atgggttgag cctgccaccc
      121 gggtcagaca ctcgcgctgc cgagccctcc cggcgtccgg catcggacaa ataaccgggc
      181 cgcgacgcta agtggcgacg ccgcagggta ggaaggctgc cgtgaccgag atagccgaag
      241 agcgccccgc cgaccgccac ccgatcgacc cgagcacctt tgacgtaccc gtcgtcgtgc
      301 tctcccatcg ggcgccggtg gcgttctacg aagagaacgg ccaccgcctg gcccgtcgcg
      361 gcgccggcgg actcgtgacg gcgctcgtcg gcctggccgc ccagctcaac gactcggtgt
      421 gggtctgcgc ggcgagcacc gacgaagaca ttgcggtggc acgcgaatac aaagacgctc
      481 cgctgcatgt cgcgatgacg acgcattctt ccatcccccc gatcaccacg gcggacgccg
      541 agggcagcct tcagattcga ttcgtcgagg tcgaccccgt cgcccacgag gacttctacg
      601 gcgtgatctc caatcccctg ctctggttcg tccagcacgg gctctacgac ctgagctact
      661 caccgaacat cgggcagcgc gagcgcgacg cgttcgaaga tggctacgtg agcgtcaacg
      721 agacgttcgc cgacgcggtc gttcacgaag tcgagcgatg cgggcgccgc gcactcgtca
      781 tgctgcacga ctaccacttc tacttggtcg cagatctggt caggaagcgt tgccctgacg
      841 tggttctctc gcatttcgtg cacattccgt ggcctgggcc tgacgcgtgg cgggtgctgc
      901 cccccgacat tcgcgagcgc ttgttctacg ggttgcttgg caacgacgtc gtcgcattcc
      961 acacggaacg cttcgcgcgc aacttcttgc tgtgcgcgca ggagttactc gggctcacga
     1021 tcgacctcga tcgcatgacg gtcgagatcg ggccgcgcac ggtgaccgca cgcacgtatc
     1081 cgatctcgat cgacgtcgcc gctttagtcg aaacggcagg cagcaaggcc gtgcgcaacc
     1141 ggctcgacga cctcgagcag tggctggtcg ccgacgacgc gaaactgatc gtgcgcgtcg
     1201 accgcaccga cccttcgaag aacatcatca gagggttcct ggccttcgag aagctgctct
     1261 gtgatcatcc ggagctcgtc ggcaaggtgc ggttcttggc gttcctccag ccgagccggc
     1321 tcgacgtccc cgaatatgcg cagtacgtgg ccgacatcgg ctcggtcgtc gcccgcgtca
     1381 acgctcgata cacgagccgg ggcgaggcgc ctatcgatct tcgtttgcac gaggacttcc
     1441 cgaccgcggt cgccgcctac acgatctgcg acgtggtcat ggtcaactcg ctcgccgacg
     1501 gcatgaacct ggtcgccaag gaggcggtat cggtgtcgag gcgcaacgcg gtgctcgcgc
     1561 tctcggagaa caccggcgcg cacgaagagc tc
//
LOCUS       DASWVF010000468         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_369186, whole genome shotgun sequence.
ACCESSION   DASWVF010000468 DASWVF010000000
VERSION     DASWVF010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369186"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1015)
                     /gene="rfbB"
                     /locus_tag="VGD55_08375"
     CDS             complement(29..1015)
                     /gene="rfbB"
                     /locus_tag="VGD55_08375"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0870400.1"
                     /translation="MRVLVTGAAGFIGSHYVRTMLSGGYPGFDDAEVTVFDKLTYAGN
                     LENLAPVSDNPRYRFVQGDICDPVAVADVLPGHDIVVNFAAESHVDRSIYGAAEFVVT
                     NVLGAQTLFDACVRAGIGRVVHVSTDEVYGTIPEGSWTEAHILEPNSPYSASKAGADL
                     LARAYAGTYGLNISVTRCTNNYGHYQFPEKVIPLFVTNLIDGLPVPLYGNGENIRDWL
                     HVDDHCRGIQLVVEKGAAGEFYNIGGGLELSNKELTQRLLDACGATWDSVVYVEDRKN
                     HDLRYSLDDAKLRAMGYAPRTSFDDGLAQTVQWYRDNQPWWRALKEKAALAK"
     gene            complement(1080..>1555)
                     /locus_tag="VGD55_08380"
     CDS             complement(1080..>1555)
                     /locus_tag="VGD55_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870401.1"
                     /translation="LFVTAAAVVVVVVPIWAGSREVIYTKTEVGEPALVRSVCAQLRP
                     NDVVLAIGGRARTEWPGTLKVMCGVNVGYLDDNIDVTQMDDIAARVTAGGGRLMLLAE
                     STADKNNAISVAAWPDKPTASVITSEATHTLVTRPGKPTVFLTEMWLGEYQPSGF"
BASE COUNT          270 a          509 c          485 g          291 t
ORIGIN      
        1 ccagccccga cgacgagcca acgatcggtc acttggcgag ggccgctttc tccttcagcg
       61 cacgccacca gggctggttg tcgcgatacc actgcacggt ctgggcgagc ccgtcatcaa
      121 atgaggtgcg cggcgcatac cccatcgccc gcagcttggc atcgtcgagg gagtagcgca
      181 ggtcgtgatt cttccggtct tcgacgtaaa cgacggaatc ccaggtcgcc ccgcaggcgt
      241 cgagcagccg ctgggtgagc tccttattcg acagctcgag cccgccgccg atgttgtaga
      301 actccccggc cgcacccttc tcgaccacga gctggatccc acggcagtgg tcatcgacgt
      361 gcagccagtc tcggatgttc tcgccattgc cgtagagagg taccggcagc ccgtcaatga
      421 ggttcgtgac gaacagcggg atgaccttct cggggaactg gtagtggccg tagttgttcg
      481 tgcagcgggt caccgagatg ttcagtccgt aggtgccggc gtatgcccgc gcaagcagat
      541 cggctcccgc tttgcttgcc gagtaagggg aattcggctc aaggatgtgc gcttctgtcc
      601 acgacccctc gggaatcgtt ccgtagacct cgtcagtcga gacgtgcacg acgcgaccga
      661 tgccggctcg cacgcacgcg tcgaacaacg tctgcgcgcc aagcacgttg gtgactacga
      721 actcggcggc accgtagatc gagcggtcga cgtgcgactc agcagcaaag ttgacgacaa
      781 tgtcgtgacc gggcagcacg tcggcaaccg ccaccgggtc gcagatgtcg ccttgcacga
      841 accggtagcg cgggttgtcg gacaccgggg cgagattctc aagattgccc gcgtaggtga
      901 gcttgtcgaa gactgtcacc tcagcgtcgt cgaagcccgg gtagccgccc gagagcatcg
      961 tgcgcacata gtgcgagccg atgaaaccgg ccgcgcctgt gaccagaaca cgcatggtcg
     1021 ctctgtcctt tcggatgttg ctgtcttcgg ggtcgcttcg ttgctgtcta ttgtgccggt
     1081 cagaacccgc tgggctggta ctcccccagc cacatctcgg tcaggaacac tgtcggtttg
     1141 cccggccgcg tcaccaaggt gtgcgtcgcc tcgctggtga tcaccgacgc cgtgggctta
     1201 tcgggccagg ccgcgaccga tatcgcattg ttcttgtccg ctgtgctctc ggcgagcagc
     1261 atcaatcgcc cgccgccggc cgttacacgg gcggcgatgt cgtccatctg ggtgacatcg
     1321 atgttgtcgt cgaggtaccc gacgttgacg ccgcacatca ccttgagggt gcctggccac
     1381 tccgtgcgtg ccctgccgcc gatagcgagc acgacgtcat tgggtcgcag ctgtgcgcag
     1441 accgagcgca ccaacgccgg ctcgccgacc tcggtcttgg tgtaaatcac ctcgcgactg
     1501 cccgcccaga tcggaaccac gaccaccacg accgcagcag cggtcacgaa caggc
//
LOCUS       DASWVF010000469         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_292811, whole genome shotgun sequence.
ACCESSION   DASWVF010000469 DASWVF010000000
VERSION     DASWVF010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292811"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..229)
                     /locus_tag="VGD55_08385"
     CDS             complement(17..229)
                     /locus_tag="VGD55_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870402.1"
                     /translation="MAKAILGHLGGPDPRMLSEMRRLQQRVRDLETECLRLQAANDAL
                     SAEVSGDELLTLSLSGSVSDQEHVYS"
     gene            complement(500..784)
                     /locus_tag="VGD55_08390"
     CDS             complement(500..784)
                     /locus_tag="VGD55_08390"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY0870403.1"
                     /translation="MTADEPVRLTAWMRGRVQGVGMRWWVRSRALELGLVGSASNLDD
                     GRVSVVAEGSREMCERLLASLRSGATPGHVTGVVERWDSPRGDFAGFTER"
     gene            complement(791..1645)
                     /gene="mutM"
                     /locus_tag="VGD55_08395"
     CDS             complement(791..1645)
                     /gene="mutM"
                     /locus_tag="VGD55_08395"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0870404.1"
                     /translation="MPELPEVETVRRGLERHVVGRRISAVEVHHPRAIRRHVAGGKDF
                     TTALTGRRIVAARRRGKYLWLPLDSGDAVLAHLGMSGQLLMLDATEPPGPHLRVRFAF
                     DDDGAELRFVDQRTFGGLAVSIDGADLPPEIAHIARDVFDPAFDDDDFHARLRLRHTG
                     LKRALLDQSLISGVGNIYADEALWRAKLHYARATETMRRPDTARLLAAVRDVMRSALE
                     QGGTSFDSLYVNVNGESGYFDRSLAVYGQAGEPCPRCGTPIRREPFMNRSSYFCPHCQ
                     PRPRNPHW"
     gene            complement(1655..2404)
                     /gene="rnc"
                     /locus_tag="VGD55_08400"
     CDS             complement(1655..2404)
                     /gene="rnc"
                     /locus_tag="VGD55_08400"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0870405.1"
                     /translation="MTDTGDPLEQLESLAQIHLDHDLLERALTHRSYAYENGGLPTNE
                     RLEFLGDSVLGVIVTETLYRAYPDLAEGQLAKLRAAVVNMRALADVSRALGLGEFIKL
                     GRGEESTGGRDKSSILADGLEALIGAVYVDGGLVAATGLVHRLFDELIDDAARLGAGL
                     DWKTSLQELTASQLLGVPYYEVTESGPDHEKTFEAAVRLGGSVFGTGSGRSKKEAEQQ
                     AAEEAWTRIREAVEATLTVDYSGDESASAAD"
     gene            complement(2440..2619)
                     /gene="rpmF"
                     /locus_tag="VGD55_08405"
     CDS             complement(2440..2619)
                     /gene="rpmF"
                     /locus_tag="VGD55_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0870406.1"
                     /translation="MPVPKRRMSRSNTRSRRAMWKTAAPTLVRCNNPACREPKLPHVA
                     CPNCGTYNRRPVASS"
     gene            complement(2622..>2884)
                     /locus_tag="VGD55_08410"
     CDS             complement(2622..>2884)
                     /locus_tag="VGD55_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY0870407.1"
                     /translation="DDEDDEIGLLVDDYVDLEPLLRDAVVLALPLAPVCRDDCLGLCP
                     DCGARLVDVEPAHGHDQVDPRWAALRAVGTTDGQRINDEQES"
BASE COUNT          480 a          956 c          959 g          489 t
ORIGIN      
        1 caatcgggta gctggatcag ctgtagacgt gctcctggtc ggataccgac ccggacaagg
       61 aaagcgtcaa caactcatcg ccggagacct cggcgctaag agcgtcgttg gccgcctgaa
      121 gacgcaaaca ctcggtctcg agatcgcgga cacgctgctg aagtcggcgc atttccgaca
      181 acatgcgcgg gtccggacca ccgagatgac cgagaattgc ctttgccatg gagatcctcc
      241 actggatgcc ggctacggtc tccaggcgca tttccgcctg gatgccgctt cagcggtgtg
      301 cgttcgtggt tacctctagg gtcgcatccg gccgcctgcc ggtcaagccc tgcgttacga
      361 tccggttacg atgctaaaag ggtctaaatc ggtctattct gggctaagaa acgcccaagt
      421 cgggcactcc cgtaaccggc cccgggcgat caacgtctcg cgcaggtctc agcgagccca
      481 tggcacccct aaatgcggct caacgctccg tgaagccggc gaaatcgccc cgtggcgaat
      541 cccatcgctc gaccaccccg gtcacgtgcc cgggtgtcgc gccactgcga agcgaggcga
      601 gcagccgttc gcacatttcc ctgcttccct cggcgaccac cgacacccgg ccgtcgtcga
      661 ggtttgaagc cgagccgacg agtccgagct cgagcgcacg cgagcgcacc caccaccgca
      721 tgccgacacc ctgcacccgg ccgcgcatcc acgcggtcag ccgaaccggc tcgtcagctg
      781 tcacggcgga tcaccagtgc gggtttcgcg ggcgcggctg gcagtgcggg cagaagtacg
      841 acgagcggtt catgaacggc tcgcgacgga tcggggtacc gcacctgggg cacggctcgc
      901 cggcctggcc gtagacagca agcgagcggt cgaagtagcc gctctcgccg ttgacgttca
      961 catagaggct gtcgaacgac gtcccgccct gctcgagggc gctgcgcatc acatcgcgga
     1021 cggcggcgag cagccgcgcc gtatctggcc ggcgcatcgt ctcggtggcc cgggcgtagt
     1081 gcaacttggc ccgccacaat gcttcgtcgg cgtagatgtt gccaaccccg gagatgaggc
     1141 tctggtcgag caacgctcgc ttcaagccgg tgtgacgcaa ccgcaggcgg gcgtggaaat
     1201 cgtcgtcgtc gaacgccggg tcgaacacgt cgcgtgcgat gtgagcgatc tcgggtggca
     1261 ggtcagcgcc gtcgatcgag actgcgagcc cgccgaatgt gcgctggtcg acgaagcgca
     1321 gttcggcacc gtcgtcgtcg aacgcaaacc gaactcgaag gtgcgggccg ggcggctccg
     1381 tggcgtcgag catgaggagt tggccgctca tgccgaggtg ggcaagcacg gcgtcgcctg
     1441 agtccaacgg gagccacaag tacttgcccc tgcggcgggc ggccacaatg cggcgtccgg
     1501 tcaaggcagt tgtgaagtcc ttaccgccag ctacgtgccg ccggatcgcg cgcgggtgat
     1561 gcacctcgac cgcgctgatg cgccggccga cgacgtgccg ctccagcccg cgtcggaccg
     1621 tttcgacctc aggaagttca ggcacgcgcg tcagtcagtc ggcagcggac gcggattcgt
     1681 cgccggagta atcgacggtc aacgttgcct cgaccgcttc cctgatacgg gtccacgcct
     1741 cttcggcagc ttgctgctcg gcctctttct tgctgcggcc tgagccggta ccgaacacgg
     1801 aaccgccaag gcgaacggcc gcttcgaacg tcttctcgtg gtcgggtccg ctctcggtca
     1861 cttcgtagta cgggacgccg agtagctggc tcgcggtcaa ttcttgaagg gaggtcttcc
     1921 aatcgaggcc tgctccgaga cgcgcggcgt cgtcgatcag ctcgtcgaag agccggtgga
     1981 ccaggcccgt tgccgcgacc aggccgccgt cgacgtagac ggcgccgatc agtgcttcga
     2041 gaccgtcagc gaggatcgac gacttgtcac ggccacccgt cgactcctcg ccgcgaccga
     2101 gcttgatgaa ctcgccgaga ccgagcgctc ggctcacatc ggcaagcgcg cgcatgttga
     2161 cgacggcggc gcggagcttg gcgagctgcc cttcggccag atcgggatag gcgcggtaca
     2221 gggtctcggt gacgatgacc ccgagcaccg agtcgccgag gaactcgagg cgctcgttgg
     2281 tgggcaggcc gccgttctcg tacgcgtaag agcgatgcgt cagcgcacgt tcgagcaggt
     2341 catggtcgag gtgtatttgg gcgaggctct cgagctgctc gagtggatca ccggtgtctg
     2401 tcatctgcgc ccccgtggca cagtgagctg ccttagccgt cagctcgatg cgaccggacg
     2461 ccggttgtac gtgccgcagt tggggcatgc gacgtgcggc agcttgggct cccggcaggc
     2521 ggggttgttg caacgcacca gggtcggggc ggcggtcttc cacatggcgc ggcggctgcg
     2581 cgtattgctg cgcgacattc tccgcttcgg aaccggcacg gctagctctc ctgctcgtcg
     2641 ttgatgcgtt gaccatcggt ggtacctact gctcgcagag cggcccatcg cgggtcgacc
     2701 tggtcgtgcc cgtgcgctgg ctcgacgtcc acgagccgcg ccccacagtc gggacacaag
     2761 cccaggcagt cgtctcggca caccggcgcg agcggtagcg cgagcaccac cgcgtcacgt
     2821 aggagcggct caaggtcgac atagtcgtca accaagagcc cgatctcgtc gtcctcgtcg
     2881 tccg
//
LOCUS       DASWVF010000470         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_369200, whole genome shotgun sequence.
ACCESSION   DASWVF010000470 DASWVF010000000
VERSION     DASWVF010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369200"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VGD55_08415"
     CDS             complement(<1..1497)
                     /locus_tag="VGD55_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0870408.1"
                     /translation="MTVGLQPGVGMKAVVMAGGEGTRLRPMTANQPKPLLPVVNRPIM
                     EHVLRLLRRHGFTDTVVTVQFLASLIRNYFGDGDELGMNLQYATEEKPLGTAGSVKNA
                     EAELRDSRFVVISGDALTDIDLTDMARFHQANNALVTVALKRVPNPLEFGIVNTAEDG
                     RIERFLEKPTWGQVFSDTANTGIYMMEPEIFNHVAAGEAVDWSQDVFPRLLDLGAPVF
                     GYIADAYWEDVGTLESLLQVQADVLNRQVDVDIEGFEMMPGVWVCEGAEVDPEAILKG
                     PLYIGNYAKVEATAELREYTVLGSNVVVKSGAFLHRAVVHDNVFIGPQVNLRGCIIGK
                     NTDIMRAARIEEGAVIGDECVVEEEAYVSAGVKVYPFKTIEAGALVNTSVIWESRGQR
                     NLFGPRGVSGLVNVEITPELAVRLASAYATTLKKGDVVTTSRDVSRAARALKRAVISA
                     LTASAINVRDLEASPEPVSRFEIAHSDSAGGIMIRTTPGDAQSVDISFL"
     gene            complement(1494..2123)
                     /locus_tag="VGD55_08420"
     CDS             complement(1494..2123)
                     /locus_tag="VGD55_08420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0870409.1"
                     /translation="MEAEQVASDRVLTVPNGLSVLRLLGVPLFLWLALGPKADGWAIV
                     VLIISSFTDWLDGKLARLLHQTSRLGQLLDPAADRLYILATVLALSVRHIMPWWLAAV
                     LIGRDVLLAGFLPILKRHGYGPLPVHYLGKAATFNLLYALPLLLLGAGHETVNHVARI
                     IGWAFAIWGTALYWWAGGLYLVQVRQLVRADRRGAAGSIDLDRAKEAAV"
     gene            complement(2199..>2417)
                     /locus_tag="VGD55_08425"
     CDS             complement(2199..>2417)
                     /locus_tag="VGD55_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870410.1"
                     /translation="RLERITDSRIGRRVNPIKDAAENSQALRDSLRATTEFEAAEVAG
                     AAPGVLAELARVRASADARVDSLRHRVA"
BASE COUNT          448 a          783 c          741 g          445 t
ORIGIN      
        1 gaggaagctg atgtcgacgc tttgggcatc acccggcgtc gtccggatca tgatgccgcc
       61 ggccgaatcg ctatgggcga tctcgaacct gctcacgggt tcgggtgaag cctcgaggtc
      121 gcgaacgttg atcgcgctcg cagtaagcgc gctgatgacg gctcgcttca aggcgcgggc
      181 agcgcgcgac acgtcacgtg aggtcgtaac gacgtcaccc tttttcaatg tcgtcgcgta
      241 tgccgaagcg agacggacgg cgagctctgg cgtgatttcg acgttcacga gccctgacac
      301 tccgcgcggc ccgaaaaggt tgcgctgccc tctggattcc cagatcacgc tggtattgac
      361 cagcgcgccg gcttcgattg tcttgaacgg gtagaccttc acacccgcag agacataagc
      421 ctcctcctcg acaacgcact catcgccgat gaccgcgcct tcttcgatcc gagctgctcg
      481 catgatgtcg gtgttcttac cgatgatgca gccacgcaaa ttgacctggg gtccgatgaa
      541 gacgttgtcg tgcacaacgg cgcgatgcaa gaacgcgccg ctcttgacga cgacattgct
      601 gccaagcacc gtgtattcgc gaagctcggc ggttgcctcg accttcgcgt agttgccgat
      661 gtagagcggg cccttcagga tcgcctcggg gtcgacctcc gcaccctcgc agacccagac
      721 gccgggcatc atctcgaagc cctcaatgtc gacgtcgacc tggcggttca gtacgtcggc
      781 ttgcacctgg agcaggcttt cgagagtgcc aacatcctcc caatacgcat cggcgatgta
      841 cccgaagacc ggtgcgccga gatcgagcaa ccgcggaaag acatcttgcg accagtcgac
      901 cgcctcgcct gcggcgacgt gattgaagat ctcgggctcc atcatgtaga tgccagtgtt
      961 cgcggtgtcg gaaaacacct ggccccatgt cggcttttcg agaaagcgct cgatgcggcc
     1021 gtcttcggcg gtgttcacga ttccgaactc gagcggattc ggtactcgct tcaaagcgac
     1081 agtgaccagc gcattgtttg cttggtgaaa ccgcgccata tcggtgaggt ctatatcggt
     1141 gagcgcatcg ccggaaatga cgacgaagcg gctatcgcgt aactctgctt ctgcgttctt
     1201 cacgctgcca gcggtgccga ggggcttctc ctccgtggcg tactggaggt tcatacccag
     1261 ttcgtcgccg tcaccgaaat agttgcgaat caaagacgcg aggaactgga ccgtcacgac
     1321 ggtgtcggtg aagccgtgcc tgcgcagcag ccgcagcacg tgctccataa tcggtcggtt
     1381 cacgaccggc agcagtggct tcggctggtt cgccgtcatc ggtcgaagcc gggtgccctc
     1441 gccgcctgcc atcacgacgg ccttcatgcc gactcccggt tgcaggccga cggtcatacc
     1501 gccgcctctt tggcgcggtc aaggtcgatc gaccctgcgg ccccacggcg atcggcgcgc
     1561 accagctgcc tgacctgcac gagatagagg ccaccggccc accagtacaa cgcggttccc
     1621 cagatggcga atgcccaccc gatgatcctg gcaacgtgat tcacggtctc gtggccggcg
     1681 cccaggagca acagcggaag ggcgtataag aggttgaacg ttgccgcctt gcccaagtag
     1741 tggactggca gcggaccgta cccgtgtcgc ttcaggatcg gcaggaatcc cgcaagcagc
     1801 acatcgcgac cgatcagcac ggcggccagc caccacggca tgatgtggcg aaccgacaac
     1861 gccagcacgg tggccaggat gtagagacgg tcggccgccg gatcaagtag ctggcccagg
     1921 cggctcgtct gatggagcaa tcgcgcgagc tttccgtcga gccagtcggt gaagctgctg
     1981 atgatgagca ccacgattgc ccagccatct gccttgggcc cgagcgccaa ccacagaaac
     2041 aacgggacgc caaggagtct gagaacgctc aggccgttgg gcaccgtgag gacacggtcc
     2101 gacgcaacct gctccgcttc cacccgacgt ccttccgcgg cccctttgtt caccccgtcg
     2161 ctcaggtctc agcttacggc ggttggggga agcggaggct aggcgactcg gtggcgcaaa
     2221 gagtcgacac gggcatcggc ggatgcccgg acccgggcga gttcggctaa gacccccggc
     2281 gcggcccccg cgacctcggc cgcctcgaat tcggtcgtcg ctcgtagcga atcccgcaat
     2341 gcctggctgt tttccgcggc gtccttgatg gggttcactc tccgcccgat gcgcgaatcc
     2401 gtaatccgct ctagccg
//
LOCUS       DASWVF010000471         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_292842, whole genome shotgun sequence.
ACCESSION   DASWVF010000471 DASWVF010000000
VERSION     DASWVF010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292842"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..1557)
                     /locus_tag="VGD55_08430"
     CDS             complement(622..1557)
                     /locus_tag="VGD55_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870411.1"
                     /translation="MLSNRFFALLAVLLLFGCPTANSDSDPTAGGAGGARSGGGAAGQ
                     QQVTKGGGGGGVSGAVGAGRAAAAAGAGGLGGAGGAAGAAASDPIEMPKLPNPDPPAP
                     PPPPPGKPIAVGCAANSECAAGYCVDGVCCNTSCDGQCFSCNQTGQIGYCAAQTTGDD
                     LNAATPCTGPRTCGAPNTVVNASACRLKDLQACKTDGDCGSLHCVTFFADHDQDGYGD
                     TSTTLKLCEEPNGVPPGGYVAVGGDCCDTDANAHPGQTQYFTKANACSSYDYNCDQAI
                     EGISGFVTYPNEPVAQCGTSVHGSCTSCTNTISCH"
     gene            complement(1599..2648)
                     /locus_tag="VGD55_08435"
     CDS             complement(1599..2648)
                     /locus_tag="VGD55_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870412.1"
                     /translation="MTAPAYGHDEPADRHRPAEGSLIGSQLRAQLLERFERQIDLPAW
                     VVAQGFHVSPVQKDPTKLAFADRHGDTLLLTKDLSAGRWLYQTAGEPVERGSIVDLMK
                     RRDGSTLDECLERMAECADLSKRSREAEAYRQASAEVTLRRAVDRHVAAQTVECDAEK
                     GLERLGVEKGAFDGWRFGAASLVLKDPPELAHSRYRESDRAMVIIERPIDAIAYERTH
                     GKQHATYIYVGDNPSDETKRKLAHVIADAPANLTVVAAVAKDRRGTALAEEIADIAGR
                     RPVERRAPEFGSRWADQMQIEGRHRASLARLHQQPDAVLENVRRGLAKALDAGVDVAQ
                     LRTAIVRRPSKGLDR"
     gene            complement(2645..2914)
                     /locus_tag="VGD55_08440"
     CDS             complement(2645..2914)
                     /locus_tag="VGD55_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870413.1"
                     /translation="MTTVVGRSQDIERRAHVYPWGIQIEVWRKVNTLTYPNTARSLPL
                     DLFLTDPRAVQEAVPVDDDPTLETEALEDYPADGRLGGPTEEPQR"
     gene            complement(2911..3492)
                     /locus_tag="VGD55_08445"
     CDS             complement(2911..3492)
                     /locus_tag="VGD55_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870414.1"
                     /translation="MDAARNKLITEHLDIAHAIAHQIANEFYLQHVFDDLLGYANQGL
                     VEAAGRYSPSADTRFRTFCWTRVRGAVIDGIRKQFFGRRKMSDGGRPSHVSYLAKGTS
                     GQPRATPVPLHDPEAALDYRRVRTALVQALAALPEAQREIVIRKHCRNELIHEIAADL
                     GIHRSNAARQHMAGLRALRSALGELGAGEEACQ"
     gene            complement(3664..>4231)
                     /locus_tag="VGD55_08450"
     CDS             complement(3664..>4231)
                     /locus_tag="VGD55_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870415.1"
                     /translation="MVISLPPLRERREDIGLLFLHCVRKALRRGDDRLSDDINVRDEW
                     LPARAVAAICCHPLPGNVRNLEGLAAKVSVEAKAFPVVVAKLKEFLLSSERGEPVRGK
                     LSTMTLAEIVEVLHQTAWSMSQAARTLGVEPSSVSRRLRKDPHIHAFAKLSVADLERR
                     LERTDGDVAALARQLGVSIELLSRRIGR"
BASE COUNT          642 a         1327 c         1523 g          739 t
ORIGIN      
        1 agcaggtttg accgcacgcg gaatcgcagc acacgccgtc cgtgcagaac ccggagccac
       61 aatcgctgtt ggagccgcag gccgtcccga gagggttagg cgtcttgcag acaggcgcgc
      121 tcccgcccgg cgccggcggg aagcagagcg cggacccagc gcacggagag gtggcggttg
      181 tgtcttgccc gctcgtgaga gcggtgcaat ggccgacggc accccgctgg ttgcaggcgt
      241 agcaggtttg gccgcacgcg gaatcgcagc acacgccgtc cgtgcagaac ccagagccac
      301 agtcgctgtt ggagctgcag gccgtaccaa gagagctcag cggcggcggc ggaacgacga
      361 cgacgggcat tggcttggcg tccggcttgg cgttcggagt ggacgttccg gcgtcggttg
      421 ccgctggcgt tgccttggca tccgacgtag gcgagccggc gtcagcgtgc acgttgacgt
      481 gaacatcggg atggtcaccc gcagccgaat cgtgacaacc ggcgacgagg cacagcggta
      541 gagcgacgag gagagagaga gcagacttga tggtgagcat tggcacgagt cctttcgtga
      601 gcattcgcgg gtgcgggtgg gttagtggca cgagatggtg ttggtgcagg aggtgcagct
      661 gccgtgcacc gacgtgccgc actgggcgac aggctcgttc gggtatgtga cgaacccgct
      721 gatgccctcg atcgcttggt cgcagttgta gtcgtaggaa ctgcaggcgt tcgccttcgt
      781 gaagtactgc gtctgtccag ggtgagcgtt cgcgtccgtg tcgcagcagt cgccgccgac
      841 ggcgacgtac ccgccggggg gcacgccgtt cggctcctcg cagagcttca gggtcgtgct
      901 ggtgtcgccg tagccgtctt ggtcgtggtc ggcgaaaaag gtcacgcagt gcagagaccc
      961 gcagtcgccg tcagtcttgc aggcctgaag gtccttcaat cggcaggcgc tggcgttgac
     1021 gacggtgttg ggagcaccac aggtgcgcgg gcccgtgcac ggcgtcgccg cattgaggtc
     1081 gtcgcccgtc gtctgggctg cgcagtaccc gatctgtccg gtctggttgc agctgaagca
     1141 ctggccgtcg cacgaggtgt tgcagcagac gccgtccacg caatagccgg cggcgcactc
     1201 agaattggct gcgcagccca cggcgatcgg ctttcctggc ggcgggggtg gcggtgccgg
     1261 cgggtcgggg ttcgggagct tgggcatctc gatcgggtcg ctggcggccg ctccggcggc
     1321 gccgcctgcg ccaccgaggc cccctgcccc cgccgctgct gccgccctcc cggcgccgac
     1381 ggcacccgat acaccgccac cgccacctcc tttcgtgacc tgctgctggc ctgcagcgcc
     1441 gccgccagat cgcgcgcctc cagcaccgcc cgcggtaggg tccgaatccg agttcgcggt
     1501 cgggcagccg aaaaggagga gtaccgcgag caatgcgaag aatctgttcg agagcatgtt
     1561 gatgtccttg cgttggtggt tggtgacgcg ggtgtcggtt accggtcgag tcccttcgag
     1621 gggcggcgga cgatggcggt ccggagctgg gcgacatcga cgccggcgtc gagagccttg
     1681 gcgagtccgc gacgaacgtt ctcgaggacg gcatcgggct gctggtggag gcgggcgagc
     1741 gaggcgcggt gccgcccttc gatttgcatt tggtctgccc agcggctgcc gaactcgggc
     1801 gcgcgccgct cgactggccg acggccggca atgtcagcaa tctcctccgc gagtgcagtt
     1861 ccgcgccggt ccttggccac ggctgcgacg accgtgaggt tggctggtgc gtccgcgatg
     1921 acgtgcgcga gctttcgctt cgtttcatcg ctcgggttgt cgccgacgta gatgtacgtg
     1981 gcgtgctgct tgccgtgggt tcgctcgtag gcgatggcgt cgatcggacg ctcgatgatg
     2041 accattgcgc gatcactctc gcggtagcgg ctatgagcga gctcgggagg gtctttcagg
     2101 accaagctgg cggccccgaa gcgccacccg tcgaacgcgc ccttttcgac gccgaggcgt
     2161 tcgaggcctt tctcggcgtc gcattcgacc gtctgagccg cgacgtgacg gtctactgcg
     2221 cggcgaagcg tgacctcggc ggaggcctgg cggtacgcct cggcctcgcg agagcgtttg
     2281 gaaaggtccg cgcactccgc cattcgctcg aggcattcat cgagggtgct gccgtctcgg
     2341 cgcttcatca ggtcgacgat tgagccgcgc tcgacaggtt cgccggcggt ctggtagagc
     2401 catcgcccgg cgctgaggtc cttagtcagc agcaacgtgt cgccatggcg atcggcgaac
     2461 gccagcttcg tgggatcttt ctggaccggg ctgacgtgaa agccttgcgc gacgacccac
     2521 gccgggaggt cgatctgtcg ttcgaagcgc tctagcagct gcgctcgaag ctgagaccct
     2581 atgagactgc cctcagccgg gcgatggcga tctgccggct cgtcgtggcc gtaagcgggc
     2641 gcagtcatcg ctgcggctcc tccgtcggcc caccgaggcg cccgtctgcc gggtagtctt
     2701 caagggcctc cgtctcaaga gtcgggtcgt cgtcgaccgg gacggcctct tggacggcgc
     2761 gcgggtcggt caggaacagg tcaaggggaa gggaccgcgc ggtgttcggg taggtgagcg
     2821 tattgacctt ccgccacacc tcgatctgga tgccccacgg gtacacgtgg gctcgacgct
     2881 cgatatcctg gcttcggcca accacggtcg tcattggcag gcctcctcgc cggcgccgag
     2941 ctcgcccagg gcggaccgga gcgctcgcaa ccccgccatg tgctggcgag cggcgttcga
     3001 ccggtggatg ccgaggtccg cggcgatctc gtgaatcagc tcgttgcggc agtgcttgcg
     3061 gataacgatt tcgcgctgcg cctcgggcag cgcggccagc gcctggacga gggcggtgcg
     3121 gacccggcgg tagtcgaggg ccgcctcggg gtcgtggagt ggtaccggcg tagcgcgggg
     3181 ctggccactg gtgcccttgg cgaggtagga gacgtgcgag ggtcggccgc cgtccgacat
     3241 cttccggcgg ccgaagaact gttttcggat gccgtcgatg accgcgccac ggacgcgggt
     3301 ccagcagaag gtcctgaacc tggtgtcagc ggacggggaa taacggccgg cagcctcgac
     3361 gagtccctga ttggcgtaac ctaggaggtc gtcgaagacg tgctgcaggt agaactcgtt
     3421 ggcgatctgg tgagcgatcg cgtgagcgat gtcgagatgt tcggtgatga gcttgttgcg
     3481 ggcggcgtcc atcgcgagtg cctttccggc cgcgtttatc gcgggccgga ccaggacaat
     3541 cgcaaggttg gtgccacgcc cgggcgggcc gaagccggtg cgtattcgtg gggttgcgtc
     3601 cgcgagagca ccggatcggc cgttgcgcgc aacggcacgc aacgatgcgc aatgcgggct
     3661 cggtcagcgc ccgatgcgac gagagagaag ctctatggag acgccgagct gacgggccag
     3721 agcggccacg tcaccgtccg tccgttcgag gcggcgctcg agatcggcca cgctcaactt
     3781 cgcgaaggca tgaatgtggg ggtcctttcg aagccgtctc gacacgctcg acggttcgac
     3841 gccaagggtc ctagcggcct ggctcatgct ccaggcagtc tggtgcagga cctcgacgat
     3901 ctcggcgagg gtcatcgtgc tgagctttcc gcgcacgggc tcgccgcgct cgctcgacag
     3961 caggaactcc ttgagcttcg cgacaacgac cgggaaggcc ttcgcctcta cggacacctt
     4021 ggccgccaag ccttcgaggt tcctgacatt tccgggcaaa gggtggcaac agatcgccgc
     4081 caccgcgcga gccggaagcc actcatcgcg cacgttgatg tcgtccgaca ggcggtcgtc
     4141 ccctcgtcga agcgccttgc gcacgcaatg caggaagagc agcccgatat cctcccgccg
     4201 ctcacgtaac ggcggcagcg aaatgaccat c
//
LOCUS       DASWVF010000472         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_471426, whole genome shotgun sequence.
ACCESSION   DASWVF010000472 DASWVF010000000
VERSION     DASWVF010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471426"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="serS"
                     /locus_tag="VGD55_08455"
     CDS             <1..1131
                     /gene="serS"
                     /locus_tag="VGD55_08455"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0870416.1"
                     /translation="FDTVRASQKTLGKQVAGTKGPDKEALLVHAKDLAAEVREAEAEL
                     ATTDEALREASLALSNIVEAGVPEGGEDDFALVATVGTVPSFDFTPRDHVELGKILGA
                     IDVERAAKVSGSRFYYLTGVGALLELALVNMAMMQAVAAGFIPMLPPALVRSRAMEGT
                     GFLGQAAENVFRLEADDYYLVGTAEVPLAAYRMDETLTADELPLRYVGFSPCFRREAG
                     TYGKDTKGIIRVHQFDKVEMFTFCAPEDAVAEHQRLLGWEREFLDALEIPYRVIDVAS
                     GDLGSSAARKYDCEAWVPTQGKYREVTSTSNCTEFQARRLNVRMRDGDGVRSAATLNG
                     TLVAIPRTIVAIMENHQQADGSVVVPAALRPYLGRDVLEPIA"
     gene            1202..1984
                     /locus_tag="VGD55_08460"
     CDS             1202..1984
                     /locus_tag="VGD55_08460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0870417.1"
                     /translation="MVATDLDGTIVRTDGTISTRTVRALLAADKAGATIVLVTGRPPR
                     WITTIAEQTDHHGIVICSNGAIVYDLHTEQILESTLIPIETVVKVVDRLSTALPDLGF
                     AVENGFDVYRSHTYRGGWNLGPDVALPVHDLDVVTSHAAAKLLASHPEIAADELLVIA
                     RGLVGDLVEPTHSNGRGLVEMGPLGVTKGTALASLAASRGIDRADVVAFGDMLNDLPM
                     LVWAGAGYAMANAHADVLAAVDLTTASNDDDGVAQVLEQLFG"
     gene            complement(2025..>2398)
                     /locus_tag="VGD55_08465"
     CDS             complement(2025..>2398)
                     /locus_tag="VGD55_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEY0870418.1"
                     /translation="IGNMIRQLLEEVRSAPLDEAGRARLKEIHAASVRELESGLSPEL
                     VAELERITLPFSESEVPSESELRIAQAQLVGWLEGLFHGIQTALVAQQMAANAQVEQM
                     RRALPQGTAPEAQRPNQGMYL"
BASE COUNT          398 a          774 c          806 g          420 t
ORIGIN      
        1 ttcgacacgg tgcgcgcctc gcagaagacg ctgggcaagc aggtcgccgg cacaaagggg
       61 ccagacaagg aagccctgct tgtgcacgcc aaggatctcg cggctgaggt tcgcgaggcg
      121 gaagcagagc tcgcgaccac cgacgaggcg ctgcgcgagg cgtcgctggc gttgtccaac
      181 attgtcgagg cgggcgtgcc agaaggtggc gaagacgatt tcgctctggt cgcgaccgtc
      241 ggcactgtgc cgtccttcga cttcacgccg cgcgatcacg tcgagctcgg caagatcctc
      301 ggggccatcg acgtcgagcg cgccgcaaaa gtttcgggct cgcgcttcta ctacctgacc
      361 ggcgtcggtg cgctgctcga actcgcgctg gtcaacatgg cgatgatgca ggcggtggcg
      421 gcgggcttca tcccgatgct gccgccggcg ctcgtgcgtt cgcgggccat ggagggcacc
      481 gggtttcttg ggcaggccgc ggagaacgtg ttccggctcg aagccgacga ttactacttg
      541 gtcgggacgg cggaggtgcc gctcgcggcg tatcgcatgg acgagacact gacggccgac
      601 gagctgccgc tgcgctacgt cggattttcg ccgtgcttcc ggcgtgaggc gggcacctac
      661 ggcaaggaca ccaagggcat catccgggtg caccagttcg acaaggtcga gatgttcacg
      721 ttctgcgcac ccgaggacgc cgtcgcagaa catcagcgtt tgctgggctg ggaacgggag
      781 tttcttgatg cgctcgaaat cccgtaccgg gtgatcgacg tcgcctcggg cgacctgggt
      841 tcaagcgccg cccgcaagta cgactgcgaa gcgtgggttc cgactcaggg caagtatcgc
      901 gaggtcacct cgacgtcgaa ctgcacggag ttccaggctc ggcgactcaa cgtgcggatg
      961 cgcgacggcg acggcgtgcg gtctgccgca acgctgaacg gcacgctggt cgcgatcccc
     1021 cgcacgatcg tcgcaatcat ggaaaaccat cagcaggcgg acggttcggt cgtggtaccc
     1081 gctgccctgc gaccctatct cgggcgcgac gttctcgaac cgattgcgta ggccgcctgt
     1141 gtcgagcgag gtgccggtgt ctcggagttc cgggctgccc acgcgacctg gactgcccaa
     1201 gatggtcgcg acggatctcg acggcaccat cgtcaggacc gacggcacga tctcgacccg
     1261 cacagtgcgc gcgctgctcg ccgctgacaa ggccggcgcg accatcgttc tcgtcaccgg
     1321 tcggccgccg cgctggatca ccacgatcgc cgaacagacc gatcaccacg gcatcgtgat
     1381 ctgctccaac ggcgcgatcg tctacgacct gcacaccgaa caaatcctcg agtcgacgct
     1441 gatcccgatc gagaccgtgg tgaaggttgt cgacaggctt tcgaccgcgc tgccggacct
     1501 cggctttgcc gtggagaacg gcttcgacgt gtacagatcg cacacgtacc gaggcggctg
     1561 gaacctcggg cccgatgttg cgttgccggt tcacgatctc gacgtggtca cctcgcacgc
     1621 ggcagcgaag ttgctggctt cgcaccccga gatcgcggcc gatgagttgt tggtcatcgc
     1681 tcgcgggctg gtcggcgacc tggtcgagcc aacgcattcg aacgggcgcg gactcgtcga
     1741 gatgggtccg cttggagtca ccaaaggcac cgcgttggcg agccttgcgg cgagtcgtgg
     1801 gatcgaccgc gccgacgtgg tcgccttcgg cgacatgctc aacgacctac cgatgctggt
     1861 gtgggccggc gccggatatg cgatggcgaa cgcgcacgcc gacgttcttg cagctgtcga
     1921 tctgacgacc gcaagcaacg acgacgacgg cgttgcacag gtgctcgagc agctcttcgg
     1981 ctagcccatt cagcgtcggg ggccgagggt gcacccctga ccgcctagag gtacatcccc
     2041 tggttcgggc gttgcgcttc gggggccgtt ccttgcggga gtgcgcgtcg catctgttcg
     2101 acttgggcat tcgcggccat ttgttgggcc acgagtgcgg tctgaatccc gtggaacaga
     2161 ccttcgagcc agccaacgag ctgcgcctgg gcgatccgca gttctgattc gctcggaacc
     2221 tcagactcgc tgaacggaag cgtgatgcgc tcgagctcgg cgaccagctc gggagacaga
     2281 ccgctctcga gctcccggac cgacgccgcg tggatctcct tcaaccgcgc ccggccggct
     2341 tcgtcgagcg gcgcagaccg tacttcttcg agcagctgcc ggatcatgtt gccgatcc
//
LOCUS       DASWVF010000473         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_165236, whole genome shotgun sequence.
ACCESSION   DASWVF010000473 DASWVF010000000
VERSION     DASWVF010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165236"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VGD55_08470"
     CDS             complement(<1..996)
                     /locus_tag="VGD55_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0870419.1"
                     /translation="MTTPQDVHSADIANAGALQEAARRHLWMHFTRMSSYTHNDVPII
                     VRGEGAYVYDSRGRRYLDGLAGLFVSMVGHGRDEIAEAAARQSKELAYFPLWSYAHPR
                     AIELAERLANLAPGDLNRVFFTTGGSEAVESAWKLARQYFRAIGQPSRYKVISRDIAY
                     HGATMGALSITGLPAIKNVFEPLVPGSIRVPNTNFYRAPEHGDDLVAFGRWAADEIER
                     AILREGPESVAAVYLEPVQNSGGCFPPPPGYFQRVREICDRHDVLLVSDEVICAFGRL
                     GHMFAADRYGYVPDMITCAKGMTSGYSPIGACLISDRVAEPFLTGDTMFAHGITFG"
     gene            complement(993..1472)
                     /locus_tag="VGD55_08475"
     CDS             complement(993..1472)
                     /locus_tag="VGD55_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0870420.1"
                     /translation="MTPRERPVASAGVLDEVSKAIIEQLQQDGRRSYATIAQAVGLSE
                     AAVRQRVQRLLKSGVIQIVAVTDPLQLGFPRQAMIGLRVEGDIEAVADELEKFPEVDY
                     VVVTAGSFDLLVETVCEDDDHLLELLNGRIRAIPAVRSTETFVYLKLRKQTYTWGTR"
     gene            1639..>2798
                     /locus_tag="VGD55_08480"
     CDS             1639..>2798
                     /locus_tag="VGD55_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0870421.1"
                     /translation="MAAGPQVLRNFVDGAYVDTEAGVTSGIVDPSTGEVFATAPVSEK
                     ADVDRAMRAAAKGFEVWRDATPSVRQRALLRIADAIEARADEIVAAESQNTGKPLALT
                     ASEELPPMVDQIRFFAGAARVLEGKAAGEYMAGFTSFIRREPVGVCAQVTPWNYPMMM
                     AVWKWAPAIAAGNAVVLKPSDTTPVTALLMAEIAAEFLPPGVLNVVCGERDTGRLLVE
                     HRTPSMVSITGSVRAGMEVAGAAARDLKRVHLELGGKAPVVVFDDADLEKTAEGIAMA
                     GYFNAGQDCTAATRVLAGPGIYGDFVAALTEQARGTVVGPPSNADAAYGPVNNANQLA
                     RVSGFVDRLPDHAQLLAGGHRIGDRGYFYEPTVVAGLRQGDEIVTDEVFGPV"
BASE COUNT          488 a          863 c          949 g          498 t
ORIGIN      
        1 gccgaaggtg atgccgtggg cgaacatcgt gtcgccggtc aggaacggct cggccacccg
       61 atcggagatg aggcaggcgc cgatggggga gtagcccgac gtcatgccct tggcgcaggt
      121 gatcatgtcc ggcacgtagc cgtagcggtc agcagcgaac atgtggccaa ggcgaccgaa
      181 cgcgcagatc acctcgtcgg acaccagcag cacgtcgtgg cgatcgcaga tctcgcgcac
      241 ccgctggaag tagccaggcg gcggcgggaa gcagccgccc gagttctgca ccggctcgag
      301 gtagaccgca gcgaccgact cgggcccttc ccgaaggatc gcgcgttcga tctcgtcagc
      361 agcccagcgg ccgaacgcga cgaggtcgtc gccgtgttca ggggcgcgat agaagttggt
      421 gttgggaacg cgtatcgatc cggggaccag cggttcgaac acgttcttga tggcgggcag
      481 gccggtaatc gacagcgcgc ccatggtggc accgtggtag gcgatgtctc ggctgattac
      541 cttgtaccgg ctcggctggc caattgcccg gaagtactgg cgagccagct tccatgccga
      601 ttcgacggcc tcggacccgc cggtggtgaa gaagacgcgg ttcagatcgc cgggtgcaag
      661 gttcgccaac cgctcggcca gttcgatcgc gcgtgggtgc gcgtagctcc acaacgggaa
      721 gtacgcgagt tctttcgatt gacgtgcggc ggcctcggcg atctcgtcgc ggccgtgacc
      781 gaccatgctg acgaacaaac cggcaagtcc gtcaaggtaa cgacggcctc tgctgtcgta
      841 cacgtacgcg ccttcgccgc ggacaatgat cggcacgtcg ttgtgcgtgt acgacgacat
      901 gcgagtgaag tgcatccaga ggtggcggcg agctgcctct tgcaacgccc cggcgttggc
      961 gatgtcggcg ctgtggacgt cttgtggcgt ggtcatctgg tcccccaggt gtatgtctgc
     1021 ttacgaagct tgagataaac gaatgtctcg gtgctgcgga cggccgggat ggcccgaatc
     1081 cgaccgttca gaagctcgag caagtggtcg tcgtcctcgc agacggtttc gaccagcagg
     1141 tcgaacgagc ccgcggtgac cacgacgtag tcgacctcgg gaaacttttc cagctcatcg
     1201 gccacggcct cgatgtcgcc ctcgacgcgc aatccgatca ttgcctgccg cgggaacccc
     1261 aactggagcg gatccgtcac ggccacgatc tgaatgacac cgctcttgag caaccgctgt
     1321 acgcgttgcc gcactgccgc ttctgacaac ccgacggctt gcgcgatcgt cgcgtaggat
     1381 cgtcgtccgt cttgttgcag ttgttcgata attgctttcg agacctcgtc gaggacgccg
     1441 gcagatgcca ccggtcgttc gcgtggggtc attcggcaat catggccccg acccagacgc
     1501 ggtgcaagcg attccgtagt cggcggaccg tgcgcccacg gaatcgatgg tcgaccctcg
     1561 atatcactga tgttctcgtc tcccgcaagc gctctcgccc agctacgctg tcggcaccct
     1621 cgccgtaagg agatagagat ggccgctgga ccacaagtgc tgcgcaattt cgtcgatggc
     1681 gcgtacgtcg acactgaggc gggcgtgacc agcggcatcg tcgacccgag caccggggag
     1741 gtatttgcga ccgcgccggt gtcggagaag gccgacgtcg atcgcgccat gcgcgcggcc
     1801 gcgaagggct tcgaggtctg gcgcgatgcc acgccgtccg tcaggcaacg tgcgctgctg
     1861 cgcattgccg acgctattga ggcccgggct gatgagattg ttgccgcgga atcgcaaaat
     1921 actggcaaac cgcttgcgct cacggcctca gaagagctgc cgccgatggt cgaccagatc
     1981 cgcttcttcg cgggcgccgc gcgggtgctc gaaggtaagg ccgcgggtga gtacatggcc
     2041 ggcttcacgt cgttcatcag gcgtgagccg gttggtgtgt gcgcgcaggt gacgccctgg
     2101 aactacccga tgatgatggc cgtctggaag tgggcaccgg cgattgcggc aggcaacgca
     2161 gtcgtgctta agccgtcaga cacgacgccc gtgaccgcac tgttgatggc tgagatcgca
     2221 gcggagttcc tgccaccggg cgtgctgaac gtggtgtgcg gcgagcgtga cacgggtcgc
     2281 ctgctggtcg aacatcgcac gccgagcatg gtctcgatca ccggcagcgt gcgcgcagga
     2341 atggaggtgg cgggtgcggc cgcgcgcgat ctcaagcgcg tgcacctcga actcgggggt
     2401 aaggctcccg tcgtcgtgtt cgatgacgcc gacctcgaaa agaccgccga gggcatcgcg
     2461 atggccggct acttcaacgc tggccaggac tgcaccgccg ccacccgagt gctggccgga
     2521 ccggggatct atggcgactt cgtcgcggca ttgacagaac aagctcgcgg cacagtcgtc
     2581 ggcccgccgt ccaatgccga tgcagcgtac ggcccggtca acaacgcgaa ccagcttgcg
     2641 cgcgtaagcg gattcgtcga ccggttgccc gaccatgcgc agttgttggc aggtgggcac
     2701 cgcatcggtg accgtggtta tttctatgag ccgacggtgg ttgccggtct gcggcagggc
     2761 gacgagatcg tgaccgatga ggtcttcgga cccgtgat
//
LOCUS       DASWVF010000474         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_318108, whole genome shotgun sequence.
ACCESSION   DASWVF010000474 DASWVF010000000
VERSION     DASWVF010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318108"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGD55_08485"
     CDS             <1..278
                     /locus_tag="VGD55_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870422.1"
                     /translation="LSAMRATRRGRTSEGYAAFRETAHVVPALTPTLLVAYLPPVAAL
                     GRKAYAWVAAHRSTTGSCAVEPGGAPAAAIAPTARTGGDTGSSKDRE"
     gene            275..1207
                     /locus_tag="VGD55_08490"
     CDS             275..1207
                     /locus_tag="VGD55_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870423.1"
                     /translation="MNDSDADVAELAGTAPAVTVIMPTYNRASYVGEAIDHLLELDSP
                     AGGFEIVVVDDGSRDNTPEVLAAYEQLPNVRIMRRVNGGPAAARNTGIGASRGQVLAF
                     TDDDCRPIQEWLTYLLAALDSTKAAGVGGRTIPASATSLVSRFVARRHLSELPNAVDG
                     EVRWLVTCNAAFHREALVAIGGFDESYPVPGGEDTDLCSRLRGAGYRLGVAPDALVRH
                     DHTWRSVRQFADTWYRYGRGDARHLRRDSQPLQPIRRVAADVRYLPTIARNLHADFSD
                     GVAAREAMAFAGLDILRRTSKTVGLVRAYARPSG"
     gene            1209..>2011
                     /locus_tag="VGD55_08495"
     CDS             1209..>2011
                     /locus_tag="VGD55_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0870424.1"
                     /translation="MSVTEPAARPQSDAKSDLQSGNGADLIDRGAGALSTRAGRGVVF
                     TLSSQWVKFVLQLIATVVIARMLDPKDYGVVGEVVALTGFADQLRELGLSDASVQRDG
                     LTRDQLDALFWINTALGLGFAAVFALGSPLVAAFYHRPELVPITLVLSLNFVAGGLSV
                     QHSALLRRRLRFGRLALSDAGPMAVSIVVGIVAAAEGARYWALVAMYLTQGFGRLAVL
                     WIGTDWWPGRPRRARGLHELLTFGADLSFFNVLNYITRNLDNVLIGRYRG"
BASE COUNT          291 a          653 c          665 g          402 t
ORIGIN      
        1 gcctatcggc gatgcgtgcg actcgtcgcg gtcgcaccag cgagggttat gccgcgttcc
       61 gtgagaccgc acatgtcgtc cccgctctga caccgacctt gctagttgcc tacctcccgc
      121 ccgttgccgc gcttggccgc aaggcctacg cgtgggtggc tgcgcatcgc tcgacgacag
      181 gatcgtgcgc agtcgaacca ggcggtgcac cggcggccgc gattgcaccg acggcacgca
      241 ctggtggcga cacgggttct agcaaggatc gagagtgaat gactccgacg cagatgtggc
      301 tgagctagca ggcacggcac ccgctgtcac cgtcatcatg cccacgtaca accgtgcgtc
      361 gtacgtgggc gaggcgatcg atcacctgct cgagctcgac agcccggcgg gtggtttcga
      421 gatcgtcgtt gtcgacgacg ggtctcgcga caacaccccc gaggtgctcg ccgcctacga
      481 gcaactcccc aacgtgcgca tcatgcgacg ggtcaacggc gggccggctg cggcccgcaa
      541 caccggcatc ggtgcgagcc gcggccaggt gctcgcattc accgatgacg actgccggcc
      601 gatccaggag tggctgacgt atctgctagc agcgcttgac tcgactaagg cggctggcgt
      661 cggcggtcgc acgattcccg catcggccac gtcgttggtg tcgcggtttg tcgcccggcg
      721 ccatctgtcg gaactgccga acgcggttga cggcgaggtt cgttggctcg tcacgtgcaa
      781 cgccgcgttc caccgcgagg cacttgtagc gatcggtgga ttcgacgagt cctatcccgt
      841 tccgggcggc gaagacactg acctgtgctc gcggttgcgg ggcgcggggt atcgcctcgg
      901 tgtggccccc gacgcgctcg tgcgtcatga ccacacgtgg cgctcggtca ggcagttcgc
      961 cgatacctgg taccggtacg ggcgtggcga cgcgcgtcat ctacgtcgcg attcgcaacc
     1021 gttgcaaccc attcggcgcg tggcggctga cgttcgctat ctcccgacca tcgctcgcaa
     1081 tctgcatgcc gacttttccg atggcgttgc cgcccgagag gcgatggcgt tcgccggact
     1141 cgacatcctc cgtcgcacca gtaagacggt cgggctcgta agggcctacg cgaggcctag
     1201 cggctgacgt gagtgtgacc gaaccggcgg ccaggccgca gtcagacgcg aagtcagact
     1261 tgcagtcagg caacggggcc gatctcatcg accgtggtgc gggcgctctg tctacgcggg
     1321 cgggccgcgg cgttgtgttc acgctgagtt cgcaatgggt gaagttcgtc ctccagctca
     1381 tcgcaacggt cgtcatcgcc cgcatgctcg acccgaagga ctatggcgtc gtcggtgagg
     1441 tcgtagcgct gaccgggttt gccgaccagc ttcgcgagct cgggctttcc gatgcttccg
     1501 tgcaacgcga tggcctcacc cgtgaccagc tcgatgcctt gttctggatc aacactgccc
     1561 tcgggctcgg gttcgctgcc gtgttcgcgc tgggatcgcc tttggtggcc gcgttctacc
     1621 accgcccaga gcttgtgcct atcacgctcg tgctcagtct caacttcgtc gcgggcggcc
     1681 tgtcggtgca gcactcggcg ttgttgcgtc gccgcttgcg ctttggtcgg ctcgcgttgt
     1741 cggacgctgg cccgatggca gtcagcatcg tcgtcgggat cgtcgcggcg gccgaaggcg
     1801 cccgttactg ggcgctggtt gcgatgtacc tgacgcaagg atttggccgg ctcgcggtct
     1861 tgtggatcgg taccgactgg tggcctggtc gtccgcgacg tgctcgtggg cttcatgagc
     1921 tgctgacgtt cggcgccgat ttgtcattct ttaacgtgct taactacatc acgcgcaacc
     1981 tcgacaacgt tctgatcgga cgttatcgcg g
//
LOCUS       DASWVF010000475         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_318155, whole genome shotgun sequence.
ACCESSION   DASWVF010000475 DASWVF010000000
VERSION     DASWVF010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318155"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VGD55_08500"
     CDS             <1..682
                     /locus_tag="VGD55_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEY0870425.1"
                     /translation="RVFTLKSEDKVAVITELAARPARTLIFVRTKHGADRLAMQLARG
                     GVHAAAIHGNLKQNARQRALDGFAAGHPRVLVATDVAARGIHVDDVDLVVHFDPPNDS
                     KDYLHRSGRTARAGASGTALSLVQPGEVRDVAALHKGAGVSPVAQDVHPGHHAVRELA
                     ASGEPVVVRVVAHRDTQTTTSRSATGRTGGGRTAPSRSGSSSSARRGGSSAPFQGSAR
                     RPRPAGVR"
     gene            728..2095
                     /gene="hisD"
                     /locus_tag="VGD55_08505"
     CDS             728..2095
                     /gene="hisD"
                     /locus_tag="VGD55_08505"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0870426.1"
                     /translation="MFDVARRPEAAESRMLRRIDLRGRAPGTIEIRALLPRAPIDVDS
                     VLESVRPICDDVFKRGANAAREITRRFDDVDLDSLRVPADALADALANLDPLVRAALV
                     EAARRARLVHTAQLRDDVDVEVARGGRVRERWVPVRRVGLYVPGGLVAYPSSVVMNVI
                     PAQVAGVESIALASPPQKAFGGLPHPAVLAACALLGVDEVYAVGGAQAIALLAYGSTA
                     ENGEAVEPVDVVTGPGNVYVAAAKRIVKGVVGIDSEAGPTEIAILADESAEPAHVAAD
                     LIAQAEHDELASCLLVTDSADLLAKVGIELDRQVASTRHIDRVKAALAGQSAYVLVDD
                     IDAGIRVVDAWAAEHLEVVTRDAPAVAARIRNAGAIFVGPWAPVSLGDYLAGSNHVLP
                     TGGTARFTGGLSVQAFQRMIHVVDYDRAALAEVAPLIDALGGAEDLAAHVAAVRARIP
                     KDAHS"
     gene            2092..3222
                     /locus_tag="VGD55_08510"
     CDS             2092..3222
                     /locus_tag="VGD55_08510"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0870427.1"
                     /translation="MSGLDSLPLRDDLRGEVPYGAPQIDVPVRLNVNENPYPPSPSLI
                     ADIAAAVGAAAGELNRYPDREAIALRRDLAGYLKDFEKVDVDPARVWAANGSNEVMHQ
                     LLLAFGGPGRCVLGFAPTYSMYPQYCRDTFTTYETVERRADFTLDAEVAIAAVRDRRP
                     VVTLLTSPNNPTGTTLPLATARAVLDVAAEHGGLVVVDEAYAEFRRPGTPSALELLDE
                     YPHLVVTRTMSKAFAYAGARVGYLVADSGVVDAVRIVRLPYHLSSVTQAVASAALAHA
                     DELLAQVDTLRAERDATVEWLRAQAFSVADSDANFVLFGEFADRHAVWQGLLDHGVLI
                     RETGPRSWLRVSIGTAGEMAAFKQALLAVAPAPVEPLGKVEA"
     gene            3219..>3548
                     /locus_tag="VGD55_08515"
     CDS             3219..>3548
                     /locus_tag="VGD55_08515"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEY0870428.1"
                     /translation="MSRNARVQRTTKESDVLVELELDGSGAVDIETGVPFYDHMLAQL
                     GKHGGFDLTVRTAGDIEVDAHHTVEDTALALGQALREALGDRAGIRRFGDALVPLDET
                     LCQVAVDL"
BASE COUNT          521 a         1222 c         1219 g          586 t
ORIGIN      
        1 ccgggtcttc acgttgaagt ccgaagacaa ggttgctgtc atcaccgagc tcgctgcacg
       61 cccggctcgc acgttgattt tcgtgcggac caagcacggc gctgaccggc tcgcgatgca
      121 gctggcccgg ggtggcgtgc atgctgccgc gattcacggc aacctgaagc agaacgcgcg
      181 ccagcgggcg ctcgacggat tcgccgccgg ccacccgcgg gtgctcgtgg cgaccgatgt
      241 cgcagcacgc ggcatccacg tcgacgacgt tgacctggtc gtgcacttcg acccgccgaa
      301 cgattcgaag gactacctgc accggtcggg ccgtacggcc cgagcgggcg ccagtggtac
      361 tgcgctctcg ctcgtgcagc ccggtgaggt gcgcgacgtc gctgccctgc acaagggtgc
      421 cggggtcagc ccggtcgccc aggacgtgca tcccggtcac catgccgttc gcgagctggc
      481 cgcttcaggt gagcccgtcg tcgtccgcgt cgtcgcccat cgcgacacgc agaccacgac
      541 cagcagatcc gcgaccggca gaaccggtgg gggccggacg gcgcccagcc ggagcgggtc
      601 gagttcgtcc gccaggcggg gtggctcgtc cgcacctttc caaggctctg cacggcggcc
      661 ccggccggcc ggcgttcgct aattggctgg cggcgcgccg gtaggctgag cgcttacgcc
      721 accgatcgtg tttgacgtcg ctcgacgtcc tgaggccgcg gagtcacgca tgttgcgtcg
      781 gatcgacctt cgcggtcggg ctccgggcac gatcgagatc cgcgctttgc tcccgcgggc
      841 gccgatcgac gtcgactctg tgctcgagtc ggtgcgaccg atctgcgacg acgtcttcaa
      901 gcgcggcgcg aacgcggccc gcgagatcac ccgccgcttt gacgacgtcg atctcgattc
      961 actgcgggtg cctgctgacg cgttggccga cgcgctcgcg aacctcgatc ccctggttag
     1021 ggccgcgctg gtcgaggcag ctcgacgcgc caggttggtc cacaccgcgc agctgcgcga
     1081 cgacgtcgac gtggaggtcg cgcgcggcgg ccgggtgcgc gagcgctggg tgccggtgcg
     1141 ccgcgtcggc ctctacgtgc cgggtggtct tgtcgcgtat ccgagcagcg tcgtcatgaa
     1201 cgtcatcccc gcccaggtcg cgggcgtcga gtcgatcgcg ctcgcctcgc cgccgcagaa
     1261 ggcctttggc ggacttccgc acccagccgt cctcgcggcg tgcgcgctgc tcggcgtcga
     1321 cgaggtctac gccgtcggcg gcgcgcaggc catcgccctg ctcgcctacg gcagcacggc
     1381 ggaaaacggc gaagctgtcg aacccgtcga cgtcgtcacc gggcctggca acgtgtacgt
     1441 cgccgccgcg aagcgcatcg tgaagggcgt cgtcggtatc gactccgaag caggcccgac
     1501 cgagattgcg atcctggccg acgaatccgc tgagcctgcg cacgtcgccg ccgacctcat
     1561 tgcacaggcc gaacacgacg aacttgccag ctgcctgctt gtcacagact cggccgacct
     1621 gctcgccaag gttggtatcg agctcgaccg gcaggtcgcc tcgacgcgac acatcgaccg
     1681 ggtgaaggcg gcactggccg gccagtcggc gtacgtcctg gtcgacgaca tcgacgcggg
     1741 gatccgggtc gtcgatgcat gggcagcaga gcaccttgag gtcgtcacgc gcgacgcacc
     1801 cgctgtcgca gcccgcatcc gcaacgccgg cgcgatcttc gtaggcccgt gggcgccggt
     1861 cagtttgggc gactacctgg ccggctcgaa tcacgtcctg ccgacaggcg gaaccgcacg
     1921 tttcaccggt gggctctcgg tgcaggcctt ccagcggatg attcacgtcg tcgactacga
     1981 ccgggctgcc ctcgccgaag tcgctccgct gatcgacgcc ctcggcgggg cagaggatct
     2041 cgctgcgcac gtggccgccg ttcgggctcg catcccgaaa gacgcgcact cgtgagcggg
     2101 ctcgactcgt tgccgttgcg cgacgactta cgtggcgaag ttccgtacgg cgccccgcaa
     2161 atcgacgtcc cggtgcgatt gaacgtcaac gagaacccgt acccgccgtc gccgtcgctg
     2221 atcgccgaca tcgcagcagc ggtgggtgcg gccgcgggcg agctgaatcg ctatcccgac
     2281 cgtgaggcca tcgcgttgcg ccgcgacctc gcgggctatc tcaaagactt cgagaaggtc
     2341 gacgtcgatc ctgcgcgggt ctgggcggcg aacggttcga acgaagtgat gcaccagctg
     2401 ttgctggctt tcggcggccc tggccgttgc gtgttggggt tcgcgccgac gtactcgatg
     2461 tatccgcagt actgccgtga cacgttcacg acgtacgaga cggtcgagcg ccgcgccgac
     2521 ttcaccctcg atgccgaggt tgccatcgct gcggtacgcg accgccgtcc cgtcgtcacg
     2581 ttgctcacct cgccgaacaa cccgaccggc accacgttgc cgctggcgac cgcacgcgcg
     2641 gtgctcgacg tcgcggcaga gcacggcggc cttgtcgtcg tcgacgaggc gtacgcggag
     2701 tttcggcgcc cgggcacgcc aagtgcgttg gagctgctcg acgagtaccc ccacctggtc
     2761 gtcacccgca cgatgagcaa ggcgtttgct tatgcgggag ctcgggtggg ctacctagtc
     2821 gccgacagcg gcgtcgtcga cgcggtgcgg atcgtccggc ttccctacca cctctcgtcg
     2881 gtgacccaag cggtcgcgag tgcggcgctc gcacacgccg atgagttgct tgctcaggtc
     2941 gacaccttgc gcgccgaacg ggacgccact gtcgagtggc tgcgcgcgca ggcgttctcg
     3001 gtcgcagact ccgatgccaa cttcgtgctg ttcggtgagt tcgccgatcg gcacgccgtg
     3061 tggcagggtc tgctcgatca tggcgtgctg attcgcgaga ccgggccgcg gtcgtggctt
     3121 cgagtcagca tcggcacggc cggcgagatg gccgcgttca agcaggccct gctcgcagtc
     3181 gccccggcgc cagttgaacc gttaggaaag gtcgaagcgt gagccgaaat gcacgagtac
     3241 agcggacgac gaaagagtcg gacgtgctcg tcgagcttga gcttgacggt agcggtgccg
     3301 tcgacatcga gactggggtg cctttctacg accacatgct ggcccagctc ggcaagcacg
     3361 gcggcttcga cctgacggtg cgtaccgcgg gtgacatcga ggtcgacgcg caccacaccg
     3421 tcgaggacac ggcgctcgcc ctcggtcagg cgctgcggga ggcactcggc gacagggccg
     3481 gcatccgcag gttcggcgat gcgctggtgc cgctggacga aacgctctgc caggtcgccg
     3541 tcgacctg
//
LOCUS       DASWVF010000476         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_446143, whole genome shotgun sequence.
ACCESSION   DASWVF010000476 DASWVF010000000
VERSION     DASWVF010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446143"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..2531
                     /gene="dnaB"
                     /locus_tag="VGD55_08520"
     CDS             204..2531
                     /gene="dnaB"
                     /locus_tag="VGD55_08520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0870429.1"
                     /translation="MSYQARGAAVSVIELPGSSSREVDRTPPQDINAEQSVLGGMMLS
                     KDAIADVVEALHGTDFYRPAHQTIFDVVLDLYGRGEPVDAVTVAAELTKRGEIARIGG
                     APYLHTLISSVPTAANAGYYAKIVAERAILRRLVEAGTRIVQLGYSGVGNEVDDIVDR
                     AQAAIYDVTERRQTEDYSILESLLQPTMDEIEASGHRGGGLTGVPTGFADLDSLTNGL
                     HPGQLVVIAGRPAMGKALALDTPLPTPFGWTTMGEVVVGEWLMGADGRPTQVVAATDV
                     LLERPCYEVEFDDGCVIVADAQHQWLTETRASRQSGIRTTQEIAETLRGATADTRPNH
                     SVLTTLPLQLDEQRLPIGPYALGVWLCAGRSALARMTSDDAEISWHIEAEGLTLDEGL
                     EQLDRIGVLHNKHIPTQYLRASELQRRALLAGLLDSEATVSPTGTVQITVMNAQLARD
                     IHELVGSLGYRGGFTTRPVNGHTPASAYTTSFTPADNPFRLERKRLALKTRATQHTPR
                     IGSRFIVHVRPIASVPVRCVEVDNAAHLYLAGRSMIPTHNSTLGLDIARAASIRANQT
                     SVLFSLEMSKHEITMRLLSAESRIPLQTMRTGQMRDDDWVKLTRRLGEMASAPLYIDD
                     SPNMTMMEIRSKARRLKQRNELRLVIVDYLQLMTSHSKVENRQQEVSQISRSLKLLAK
                     ELEVPVIALSQLNRGSEQRTDKRPQLSDLRESGAIEQDADVVILLHREDMYERESPRA
                     GEADLIVAKHRNGPTSTIVVAFQGHYSRFVDMAAG"
BASE COUNT          558 a          873 c          854 g          458 t
ORIGIN      
        1 acggcggcga agtgacgaac gcgccttgca cacgtcgtcc acaccgtgtc cccaaggcag
       61 gagcggttgt ccccagctag cccacagatg agtccgcagc cggcccttcc cgcgcaagat
      121 gggactctct aaggtcctac aacgcaggtg tacgccgaat gcgccagcga aaatgtcgtc
      181 cgcttcacgt acgtatgtca gccatgagtt accaggcgcg aggtgcagca gtgagtgtga
      241 tcgaactccc aggttcgagt tcacgggaag tagaccgcac accgccacag gacatcaacg
      301 ctgagcagtc cgtgctcggc ggcatgatgc tgagcaaaga cgcgattgct gatgtcgtcg
      361 aagcgctcca tggcaccgac ttctaccgtc ccgcgcacca gaccatcttc gacgtcgtgc
      421 tcgacctgta cgggcgcggg gagccggttg acgccgtcac cgttgccgct gagctgacca
      481 agcgcggcga gatcgcgcga atcggcgggg cgccctacct gcacacgctg atctcgtcgg
      541 tgcccaccgc tgcgaacgcg ggttactacg cgaagatcgt cgccgagcgc gcgatcctgc
      601 ggcggcttgt cgaggccggc acccgcatcg tgcaactcgg atattcgggt gtcggcaacg
      661 aggttgacga catcgtcgat cgtgctcagg ccgcgattta cgacgtcacc gagcgccgcc
      721 aaaccgagga ctacagcatc ctcgagtcac tcctgcaacc gacgatggac gagatcgaag
      781 cgagcggtca tcgcggcggc gggctcaccg gcgtcccgac gggatttgcc gacctcgact
      841 cactgaccaa cggtttgcat cctggacagc tcgtggtgat tgccggccgc cctgcgatgg
      901 gcaaggcgct agcactcgat acgccactgc caacgccatt cggctggacg acgatgggcg
      961 aggtcgtcgt gggcgagtgg ttgatgggcg ctgacggtcg gcccacgcag gtggtcgcgg
     1021 cgacggatgt gttgctagag cgtccgtgct acgaagtcga gttcgacgac ggttgcgtca
     1081 tcgttgctga cgcgcagcac caatggctga cggagaccag ggccagccga caatctggta
     1141 tacgtacgac ccaggagatc gccgagacgc tgcgaggcgc gacagccgac acgcgcccaa
     1201 atcactcggt gctgaccacg ttgccactcc aactcgacga gcaacggctc ccgattgggc
     1261 cctacgcgct cggagtatgg ctgtgcgccg gacgcagtgc cctggcgcgg atgaccagcg
     1321 acgatgccga gatcagctgg cacatcgaag ccgaagggtt gacgctcgac gaaggcctag
     1381 agcaactaga tcgaatcggc gtgctccaca acaagcacat tccgacccag tacctgcgcg
     1441 cttcggaact gcagcgtcgg gcgttgcttg cgggcctgct cgacagcgag gcaactgtgt
     1501 cgcccactgg cactgtgcag atcacagtca tgaatgcgca attggcccgc gacattcatg
     1561 agctcgtggg cagcctgggt taccgaggtg gcttcacgac aaggccggta aacggtcaca
     1621 cgcccgcttc cgcatacacg acgtcgttta cacccgccga caatcccttc cggctcgagc
     1681 gcaaacggtt ggcgttgaag acacgggcca cacaacacac cccgcgaatc ggttcgcggt
     1741 tcatcgtcca cgtgcgcccc attgcctcgg tgccggttag gtgcgtcgaa gtcgacaacg
     1801 ccgcccacct gtacctggcg gggcggtcga tgatcccaac gcacaactcg acgctcggtc
     1861 tcgacatcgc gcgggcggcc tcgattcgag ccaaccagac gtcagtgttg ttctcgctgg
     1921 aaatgagcaa gcacgagatc accatgcgct tgctctccgc cgaatcgcgc atcccactgc
     1981 agacgatgcg cacgggtcag atgcgcgacg acgactgggt gaagctcacg cgcaggctcg
     2041 gcgagatggc cagcgcaccg ctctacatcg acgactctcc gaacatgacg atgatggaga
     2101 tccgctcaaa ggctcgacgg ctcaagcagc gtaacgagct gcggctcgtc atcgtcgact
     2161 acttacagct gatgacgtcg cactcgaagg ttgagaaccg gcagcaagaa gtcagccaga
     2221 tctcgcgatc gctgaaactg ttggcgaaag aactcgaggt ccccgtgatc gcgttgtcgc
     2281 agctaaaccg aggttctgag cagcgcaccg acaagcgacc gcaactctcc gaccttcgtg
     2341 aatccggcgc aatcgagcag gacgcggacg tcgtgatcct gttgcaccgc gaagacatgt
     2401 acgagcgcga gtcgccccgg gctggtgaag ccgacctgat cgtggcgaag caccgcaacg
     2461 gaccgacaag caccatcgtc gtcgcgttcc agggccacta cagccgcttc gtcgacatgg
     2521 ccgccggcta gcgcgacaag gtttcgaaag cagccatcta gccgctttga gtgcgcatgc
     2581 cggtagtgcc tcagataggt tcgcgtcatg gccagcaagg tgcgagtacc gcaaagaggg
     2641 atcgtcggag gctggaaggg tctcctggaa atcgacggcg acatactgcg agtggttggc
     2701 gacgaccccg cgaaccatct cgaggtcgac tgtgctcagg tgg
//
LOCUS       DASWVF010000477         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_242138, whole genome shotgun sequence.
ACCESSION   DASWVF010000477 DASWVF010000000
VERSION     DASWVF010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242138"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..566)
                     /locus_tag="VGD55_08525"
     CDS             complement(33..566)
                     /locus_tag="VGD55_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0870430.1"
                     /translation="MRSIRVLGVDPGLTRCGIGVVDSAGGGRLALIEVDVLRTPSDAE
                     IGARLLALEVGIEAWIVKHRPDVVAVERVFSQHNVRTVMGTAQAAAVAITCAARNGVP
                     VALRTPSEVKAAVTGQGRAGKDQVAFMVRKLLGAPSLVGPADATDALALAICEVWRGA
                     AAARVNMAVAATQVARR"
     gene            766..1740
                     /locus_tag="VGD55_08530"
     CDS             766..1740
                     /locus_tag="VGD55_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY0870431.1"
                     /translation="MSDVEGLRTPAAPRSSLPLASHPFTCADAINAGYTRGLIRHLVS
                     TGRWLVLRRGVYCDIALSERANRVWLHAAAALLVMTAGAAISHDTAGTLYEWPLGRRR
                     DDPSTIVGRPTIYLTHPPTSRHARRGHPGVIERVAALPASHVAYFQGLLVTTAARTIV
                     DLARFRPYTEGVAVADAALHLGVTTNAQLIAVRDACRSWPGINRASKVIEFADGAAES
                     PLESRSRVAFAIGGLPTPVLQAVIVLRNGRRARVDFLWAQWRIIGEADGRLKIQRPED
                     LWAEKLREDGLRELGYEVVRWTWDEIVNHPEVVVARILRAIARARLRG"
     gene            complement(1837..>2459)
                     /locus_tag="VGD55_08535"
     CDS             complement(1837..>2459)
                     /locus_tag="VGD55_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0870432.1"
                     /translation="ISGNPTLADAIAKAKGSSVPNENIDRAVKRGAGLEAGGADWEPI
                     TYEGYGPGGVAMLIECLTDNRNRAASEVRTAMTRNGGNLADPGSVAYLFNRKGVVLVP
                     KSGRSEDDVLLAVLEAGAEEVNDLDDSFEIVCEPTDLPAVRAALKAAGIAYESADISW
                     LASVSVQLDDDVARRVLRLVDALEDCDDVQNVWANFDVSDDVLAEA"
BASE COUNT          400 a          850 c          793 g          416 t
ORIGIN      
        1 gatcgaggcg acccgccccg acacgaaagc gatcatcgcc gagccacctg ggtggctgcg
       61 acagccatgt tgacgcgggc tgcggcagcc ccgcgccaca cctcgcagat cgcgagcgcg
      121 agtgcgtcgg tggcgtcggc cgggcctaca agacttggag cgccgagcag tttgcgcacc
      181 atgaacgcga cctggtcctt gcctgcgcgg ccttgcccag tgacagcggc cttgacctca
      241 ctcggtgtgc gcagcgcgac cggcaccccg tttcgcgccg cgcaggtgat cgcgacggcc
      301 gcggcttgcg cggtgcccat cacggtgcgg acgttgtgct ggctgaagac gcgctcgacg
      361 gcgaccacgt cgggccggtg cttgacgatc caggcttcga tgccgacctc gagtgccagc
      421 agtcgcgcgc cgatctcggc atcagagggc gtgcgcaaga cgtcgacctc gatcaacgcc
      481 agcctgccgc cgcccgcgct gtcaaccacg ccgatgccgc agcgggttag tcctgggtca
      541 acgccgagga ccctgatcga acgcatgttc gggagcctag cggcgtccgc gctcggttcg
      601 gcgcagcgac gcaccggccg cgcccgcctt tgcccacttc acatccggtg tggtcgcgat
      661 ttaggggctt aaggccggaa tccacaacca caccggatgt gaagtgctgt ccaccggtgc
      721 aactcgccgc tgcctcccca ctgcgccagc cgacatggtc aatgcatgtc ggacgtcgag
      781 gggctgcgaa cacccgctgc cccgaggtcg tcgctgccgc tcgcgagcca tccattcacc
      841 tgtgccgacg cgatcaacgc cggctacacc cgcgggctga tcaggcacct cgtcagtact
      901 ggccgctggc tggtactccg acgcggcgtc tactgcgaca tcgcactctc cgagcgggct
      961 aacagggttt ggctccatgc ggccgccgca ttgctcgtca tgaccgccgg ggcagcaata
     1021 agtcacgaca cggcaggcac gttgtacgaa tggccactgg gacggcggcg agatgacccc
     1081 tcaacgatcg tcggccgccc gacgatttac ctcacccacc cgcctacttc gcgccacgcg
     1141 cgacgcggac atccgggggt catcgaacgc gtggccgccc tacccgcttc acatgttgcg
     1201 tactttcagg gtctcctcgt caccaccgcg gcgcgcacga tcgttgatct cgcgcggttc
     1261 cggccgtaca ccgaaggcgt ggcggtggcc gacgccgcgc tgcacctggg agtaacgacg
     1321 aatgcccagc tcatcgccgt ccgtgatgca tgtcgctcct ggcccggcat caaccgcgca
     1381 tccaaggtga tcgagttcgc cgatggggca gcggaaagcc ccctcgaatc gcgctccaga
     1441 gtcgcctttg ccattggcgg gctgccgact ccagttctgc aagccgtcat tgtgctgagg
     1501 aacggccgcc gtgcgcgggt ggacttcttg tgggcgcagt ggcggatcat cggggaggcc
     1561 gacggtcgcc tgaaaatcca gcgtcccgag gacctgtggg cggagaaact ccgggaggac
     1621 ggcctgcgcg aactgggtta cgaagtcgtc cgttggacgt gggacgagat cgtcaaccac
     1681 cccgaggtag ttgtggccag aattctgcgg gcgattgcac gcgcccgact ccgcggatag
     1741 caacttcaca tccggtgtgg tcgcgaattg accgcagagt ggccgaattc acgaccctac
     1801 ggggcgtgaa gttgcgaatc ggcaagagac gaccggtcag gcttcggcga ggacgtcgtc
     1861 ggagacgtcg aagttcgccc acacgttctg gacgtcgtcg cagtcctcga gcgcgtcgac
     1921 cagtcgcagt acccgccgcg cgacgtcgtc gtcgagttgc acgctcacgc tcgccagcca
     1981 cgagatgtcg gccgactcgt aggcaatccc ggccgccttc agcgccgccc gcacggcagg
     2041 caggtcggtc ggttcacaca cgatctcgaa ggagtcatcg aggtcgttga cctcctcggc
     2101 gccggcttcc aagactgcga gcaacacgtc gtcctcgctg cgcccgctct ttggtactaa
     2161 cacgacgccc ttgcggttga agaggtacgc cacggagcca gggtcagcga ggttcccgcc
     2221 gttgcgcgtc atggctgtgc gcacttcgga cgccgcgcgg ttgcggttat cggtcaagca
     2281 ctcgatcaac atggcaacgc caccggggcc gtagccctcg taggtgatcg gttcccagtc
     2341 ggcgccgccg gcctccagac ccgcgccacg cttcaccgcg cggtcgatgt tctcgttcgg
     2401 cactgaactg cccttggcct tcgcgatcgc gtcggcaagt gtcgggttgc ccgagatgt
//
LOCUS       DASWVF010000478         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_318281, whole genome shotgun sequence.
ACCESSION   DASWVF010000478 DASWVF010000000
VERSION     DASWVF010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318281"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VGD55_08540"
     CDS             <1..907
                     /locus_tag="VGD55_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0870433.1"
                     /translation="GLRIGADMVALSFVRSAEDFADVAKIMDEEGRRVPVIAKIEKPQ
                     AVDRLEEIMEAFDGFMIARGDLGVEMPLEEVPLVQKRAIALARQNAKPVIVATQMLET
                     MFGASRPTRAEVSDVANAVLDGADALMLSGETSVGAHPSLVVQTMARIITTVEKGALD
                     TIPVVTGKARTKGGAIAHAAAEVGADLRARYLVAFTETGDSARRLSRYRSAIPLLAFS
                     PIGSTRNQLALTWGVETFLVPPVSHTDQMVRQVDRALLELERCQLGELVVIVAGSPPG
                     VPGSTNTLRVHRIGDTVETDAPAYS"
     gene            complement(920..992)
                     /locus_tag="VGD55_08545"
     tRNA            complement(920..992)
                     /locus_tag="VGD55_08545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(957..959),aa:Leu,seq:caa)
     gene            1060..>1509
                     /locus_tag="VGD55_08550"
     CDS             1060..>1509
                     /locus_tag="VGD55_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0870434.1"
                     /translation="MREDDAGAKHVPTPADIAKARQSPSRVETTARTETTGRTPTAAA
                     LPPPRSRVVIAEDEALIRLDLKETLDELGYEVVGEAADGEQAVALARELRPDLVILDV
                     KMPVLDGITAAQRIAAAGIAPVVILTAFSQRDLVERARDAGAMAYLVK"
BASE COUNT          252 a          495 c          518 g          244 t
ORIGIN      
        1 gggtctgcgc atcggcgccg acatggtcgc gttgtctttt gtgcggtcag ccgaagactt
       61 cgccgatgtc gccaagatca tggacgaaga ggggcgccgg gtccctgtca tcgccaagat
      121 tgaaaagcct caggcggtcg accggctcga ggagatcatg gaagcgtttg acggcttcat
      181 gattgcccgc ggcgacctcg gggtcgagat gcctctcgag gaggtcccgc tcgtacagaa
      241 gcgtgcgatc gcgctcgccc ggcagaacgc aaagcccgtc atcgtggcga cgcagatgct
      301 cgagacgatg tttggggcgt cgcggcctac gcgagccgag gtctccgacg ttgccaacgc
      361 cgtgctcgac ggggctgacg ccctgatgtt gtcgggggag accagcgtcg gcgcgcaccc
      421 gtcgctcgtt gtgcagacga tggcgcgcat catcacgacc gtcgagaagg gcgctctcga
      481 cacgatcccc gtggtcaccg ggaaggcgcg gacaaagggc ggcgcgatcg cgcacgcggc
      541 agcagaggtc ggtgccgatc ttagggcgcg ttacctggtc gcgttcactg aaaccggtga
      601 cagcgcgcgc cgactgtctc ggtaccggtc ggcgatcccg ttgctcgcgt tcagcccgat
      661 cggctccacc cgaaaccagc tcgcgctcac ctggggagtc gagaccttcc tggtgccccc
      721 ggtctcacac accgaccaga tggtgcgcca ggtcgaccgc gccctgctcg aactcgagcg
      781 ttgccaactc ggcgagctgg tcgtgatcgt tgctggcagc ccgccgggcg tccctggctc
      841 gaccaacact ctgcgcgtgc accgaatcgg cgacaccgta gaaaccgacg ccccggccta
      901 cagctaacaa gcagtcgggt accccgggtc ggattcgaac cgacactgga tggtgtttga
      961 gaccattgcc tctgccgttg ggctaccggg gctgccgccg gcttcccggc aaggcttgca
     1021 gtgcgcgggc caatctatcg ggccgggtag gctggccgcg tgcgggagga cgacgcgggg
     1081 gccaagcacg tgccgacgcc cgctgacatt gcgaaggccc gccaatcgcc gtcccgcgtt
     1141 gaaacgactg cgcggacgga aacaactggg cggacgccga ccgctgccgc cctaccgccc
     1201 cctcggtcga gggtggtgat cgccgaggac gaagcgctga tccggctcga cctcaaagag
     1261 actctcgacg agctgggcta cgaggtcgtt ggtgaggcag cagacggcga gcaggccgtc
     1321 gcgctggcgc gagaactgcg gcctgacctg gtgattctcg acgtcaagat gccggtgctc
     1381 gatggcatca ctgcagcgca gcggatcgcg gcagcgggga tcgcacccgt cgtgatcctt
     1441 acggcgtttt cccagcgcga cctggtcgaa cgggcccgcg acgctggcgc catggcctac
     1501 ctggtcaag
//
LOCUS       DASWVF010000479         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_191288, whole genome shotgun sequence.
ACCESSION   DASWVF010000479 DASWVF010000000
VERSION     DASWVF010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191288"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..852
                     /gene="lexA"
                     /locus_tag="VGD55_08555"
     CDS             52..852
                     /gene="lexA"
                     /locus_tag="VGD55_08555"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0870435.1"
                     /translation="MATETDASDTGSDLPAGDVRRFPDVGADGNGLTPRQRKVLEVIR
                     DSVERRGYPPSMREIGEQVGLTSTSSVAHQLTTLQKKGYLRRDPNRPRAVEVRIPGEI
                     AGTSTSEPRGTRDHSNARDHSNSREQATPFDETESGNSRPTPAYIPVVGRIAAGGPIL
                     AEQAIDDVFPLPRNVVGEGTLFMLKVVGDSMVDAAICDGDWVVVRQQPVAESGDIVAA
                     MIDGEATVKTFKRRDGHVWLMPHNPNYAPIPGDDATILGRVSAVLRKV"
     gene            906..>1559
                     /locus_tag="VGD55_08560"
     CDS             906..>1559
                     /locus_tag="VGD55_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0870436.1"
                     /translation="MDDEQVLASDGVTYVVQVGDTVRRPVRPFTATIQSFLRHLHDRG
                     FNDAPVPLGYDEQGREVLSFVAGDVPQEPLPDYACTDEVLVPLGRLIRRLHEAAADFT
                     APSDAVWGGIPGAPVGVVPLFDKPELISHQDYCPGNVVFRDGLPAAFIDFDLSRPTTR
                     VVDLVNAMYWWTSLMHPSDRPPALRDADVPARVRILADAYGMAAEQRALVVPTAVQRA
                     "
BASE COUNT          264 a          562 c          508 g          225 t
ORIGIN      
        1 agaacgcccc ggtgatcacg tgcagtccag accaacctgt ggaggccttc cgtggcgacc
       61 gagaccgacg ccagcgatac cgggtccgac ctgcccgccg gtgacgtgcg gcggttcccg
      121 gacgtcggcg ccgacggcaa cgggctgacc ccacgccagc gcaaggtgct cgaggtgatc
      181 cgcgactccg tcgagcggcg cggctacccg ccgagcatgc gcgagatcgg cgagcaggtc
      241 ggcttgacct ccacctcgag tgtcgcccac cagctgacca cgctgcagaa gaaggggtac
      301 ctgcgccgcg acccgaatcg accgcgcgcc gtcgaggtcc ggatccctgg cgagatcgcc
      361 ggcacgtcca cgtccgagcc ccgcggcacc cgagaccaca gcaacgcccg agaccacagc
      421 aactctcgtg agcaggccac cccgttcgac gagaccgaga gtggcaactc ccgcccaacg
      481 ccggcctaca tcccggtcgt cggccgcatc gccgctggtg gtccgatcct cgcggagcag
      541 gccatcgacg acgtgttccc actcccccgc aatgtcgtcg gcgagggcac gctgttcatg
      601 ctgaaggtcg tcggcgactc gatggtcgat gcggcgatct gcgatggcga ctgggtcgtg
      661 gtccgccagc aaccggtcgc cgaaagcgga gacatcgtcg cggcgatgat cgacggcgaa
      721 gccaccgtca agacgttcaa gcggcgcgac ggtcacgtgt ggttgatgcc gcacaacccc
      781 aactacgcgc cgatccccgg cgacgacgcg acgatcctcg gccgcgtgag cgccgtcctg
      841 cgcaaggtct gatcagcaag gttcgatcag caccggcgtc cctcggcccg gttagggtcg
      901 agacgatgga cgacgagcag gtactggcca gcgacggcgt cacctatgtg gtgcaggtcg
      961 gcgacacggt gcgccggccg gtacggccgt tcacggcgac gatccagtcc ttcctgcgac
     1021 acctgcacga ccgcggcttc aacgacgctc cagtgccgct gggttacgac gagcaaggcc
     1081 gtgaagtgct gtccttcgtg gccggcgacg tgccacagga gccgctgccc gattatgcct
     1141 gcaccgatga ggtgctcgtg ccccttggca ggttgatccg acggctacac gaggcagctg
     1201 ccgacttcac agcgccaagc gacgccgtgt ggggtggcat ccccggggcg ccagtgggcg
     1261 tcgtcccgct gttcgacaag cctgaactca tctcgcatca ggactactgc cccggcaacg
     1321 tggtcttccg cgacggcctg cccgcggcct tcatcgactt cgacctctcg cgcccgacga
     1381 cccgggtcgt cgacctcgtc aacgccatgt actggtggac ctcgctcatg catccaagcg
     1441 acagaccccc agccctgcgg gacgccgacg tgccggcgcg ggtgcgaatc ctcgccgacg
     1501 cgtacggcat ggcggcagag caacgggcac tcgtggtacc aacggccgtg cagcgcgcc
//
LOCUS       DASWVF010000480         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_293176, whole genome shotgun sequence.
ACCESSION   DASWVF010000480 DASWVF010000000
VERSION     DASWVF010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293176"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGD55_08565"
     CDS             <1..546
                     /locus_tag="VGD55_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870437.1"
                     /translation="WLLILLVVVIGLVIAPGARWITRRRRWTSADTEAARAHAAWAEL
                     TDDMRDLGLEWRGQTDTPRRAAATLVATRRLSTDAAAEQALSRLTRAEELARYAARPE
                     ELRWVDQDPRADETTVRKALFSSVSRGRRLRARLAPSSSSRIAVSVTAWIADAWRGAG
                     LASRRWVNRRRPGRGRDDQQD"
     gene            complement(543..1001)
                     /locus_tag="VGD55_08570"
     CDS             complement(543..1001)
                     /locus_tag="VGD55_08570"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY0870438.1"
                     /translation="MPLSEHEQRVLEQIERALYADDPKFAATVRGIDPRTLHRRRYLR
                     AALGAIVGVVLLPVGIAVQQLELSVAGFVLMLLAFLYGVSTWRQGREGAEVPEPGVAR
                     ATDLPTIRRRGKKLGTPPAPKRSIVKRFEDRWDRRRDGLGGGAPGQDDDR"
     gene            complement(1330..2088)
                     /locus_tag="VGD55_08575"
     CDS             complement(1330..2088)
                     /locus_tag="VGD55_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0870439.1"
                     /translation="MAADRLRTRGSVRTAVVWRSVRTLLVDLVDVTGREQLDVLDAGG
                     GTGGFAVPLAELGHRVTVVDASPDSLAALVRRAADAGVTDRISSVQGDAASLLDVVQP
                     ASFDFVVLHSVLEIVDDPAAALVAVGGVLRLGGLVSVVAANRVAAVLHRAAAGRFDEA
                     LHALRDPLGRYSANDPAPRRFALADLEALFAAAGLTVMATHGSRVFADLVPGGSLDAD
                     PSAVEALIALEFEASELPAFRDIATQLHVVGRRS"
     gene            complement(2119..>2381)
                     /locus_tag="VGD55_08580"
     CDS             complement(2119..>2381)
                     /locus_tag="VGD55_08580"
                     /inference="COORDINATES: protein motif:HMM:NF037499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HEY0870440.1"
                     /translation="GVRRVRRSRNAWVLLTQVAPELAEWAAFFAAGATKRAAAEAGLD
                     RSVSAREADDLLRDSATFLALAETTIGVVHQPALSIDAARAS"
BASE COUNT          422 a          815 c          804 g          340 t
ORIGIN      
        1 tggctgctca tcttgttggt cgtggtgatc ggcctcgtca tcgcgccggg tgcgcgctgg
       61 atcacccgac gacgtcgatg gacgtccgca gacaccgaag cggcacgagc ccatgcggcg
      121 tgggcggagc tcacggacga catgcgcgac ctggggctcg agtggcgtgg ccaaaccgac
      181 actcctcgaa gggccgccgc gacgttggtc gccactcgaa ggctgtcgac tgacgccgcc
      241 gcagagcagg cgctgtcacg gctaaccagg gccgaagaac ttgccaggta cgcggcacgg
      301 cccgaggagc tgcgatgggt cgatcaagat ccgcgcgccg acgagaccac cgtacgcaag
      361 gcgctcttca gttcggtgtc acgtggtcgc aggttgcgtg cgcgcctggc accgtcgtca
      421 tcgagccgga ttgcggtgtc ggtgaccgcg tggatcgccg acgcgtggcg tggtgccgga
      481 ctcgccagcc ggcgctgggt aaatcgacga cggcccgggc ggggccgcga cgatcagcag
      541 gactagcggt cgtcgtcctg tccgggtgct ccgccgccga gaccgtcgcg acgacggtcc
      601 cagcgatcct cgaaccgctt gacgatgctg cgcttcggtg cgggcggcgt gccgagtttc
      661 ttgccgcgcc ggcggatggt cggcagatcg gtcgcgcgcg caacgccggg ttcgggaact
      721 tcggctccct cgcgaccctg gcgccaggtc gacacgccgt acaggaacgc cagcagcatc
      781 aacacgaagc ctgcgacgct cagttcgagc tgctgcaccg cgatgccgac aggtagcagg
      841 acgacgccga cgatggcgcc gagggctgcc cgaaggtaac ggcgacggtg caaggtacgc
      901 ggatcgatgc cgcgaacagt ggcggcgaac ttcgggtcgt cggcgtaaag ggctcgctca
      961 atctgctcga gcactcgctg ctcgtgctca gagagcggca ccatgccctc ccaacgacat
     1021 ccccaccatg cccccgacct gccgtgcgtc cgaactcatt tgcgaccttg ctgcctatca
     1081 gtgtgcaaca cgatccgagt cgaactggct tgccgagcga cgaccgggcc ggtcgtcgca
     1141 ctttcaggat acgagcctgc cggtagcggc gaaagccggt tgggcaagcg atgacaaagg
     1201 aactgcatga gatcggcttg gttccggcct cggcaccggg gtacccagct ccggtaccgc
     1261 accaaacccg gcagcccgac cacaccacag ggtaggtatc gggtggctcg gccggcatct
     1321 ggagagccgt tagcttcgcc ggccgacgac gtgcaattgg gtcgcgatgt cccgaaacgc
     1381 tggcaactcg gaggcctcga actccaacgc aatcaacgcc tcgacggctg acgggtcagc
     1441 gtcgagtgat ccacccggaa ccagatcggc gaagacccgc gagccgtgcg tcgccatcac
     1501 cgtcaacccg gccgcagcga acaaagcttc tagatcggcg agtgcgaagc ggcgcggcgc
     1561 cggatcattg gccgaatacc ggcccagtgg gtcgcgcagg gcatgcaacg cctcatcgaa
     1621 gcgcccggcg gccgcgcggt gcagaacggc agccacccgg ttcgcagcga cgacgctgac
     1681 caggcctccc aggcgcaaga caccgcccac ggccacgagc gctgcggcgg ggtcgtcgac
     1741 gatctcgagc acgctgtgca agacaacaaa gtcgaacgaa gcaggctgga cgacgtcgag
     1801 caggcttgct gcgtcgcctt gaacactgct gatccgatcg gtcacaccgg catcggcggc
     1861 ccgccggacg agcgccgcaa gggagtccgg gctggcgtcg accaccgtca cccggtggcc
     1921 gagttccgcc agcgggaccg caaagccgcc agtgccaccc ccggcgtcga ggacgtcgag
     1981 ttgttcgcgg cccgtcacat cgaccaagtc aacgagcagc gttcgaacgc tgcgccagac
     2041 gacagccgtc cgcaccgagc cgcgggtgcg cagtcggtcg gcggccatga ggcgcacctt
     2101 accggccaca actcctcgtt agctcgctcg tgccgcgtcg atcgatagcg ccggttggtg
     2161 cacgacaccg atggtggttt cggccagtgc gagaaaggtc gcactgtcgc gcagcagatc
     2221 gtcggcctcg cgggcgctca cggatcggtc gagccccgcc tcggctgccg cacgtttcgt
     2281 cgcgccggcc gcaaagaatg cggcccactc ggcgagctca ggcgcgacct gcgtcagcaa
     2341 gacccacgca ttgcgtgatc gacgaacccg ccgaacaccg g
//
LOCUS       DASWVF010000481         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_140528, whole genome shotgun sequence.
ACCESSION   DASWVF010000481 DASWVF010000000
VERSION     DASWVF010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140528"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /gene="groL"
                     /locus_tag="VGD55_08585"
     CDS             complement(<1..1380)
                     /gene="groL"
                     /locus_tag="VGD55_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719200.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0870441.1"
                     /translation="MPKILQYDESARRSLERGVDALANAVKVTLGPKGRNVVIAKSYG
                     VPTITNDGVTIAREIELQDRHENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVHEG
                     LRNVAAGADPLALKRGIEAAVTAVTARLLDTAREVSGKGEIAHVAAISAQDPEVGGLI
                     AEAFDKVGKDGVITVEESNTMGLELELTEGLQFDKGYISPYFVSDTERMEAVLENPLI
                     LLNQGKLSSVSDLLPLLEKVIQAGKSLLVIAEDVDGEALSTLVVNKIRGTFSSVAVKA
                     PAFGDRRKSMLEDIAILTGAQVVAPEVGLKLDDVGLEVLGTARRVTITKDDTTIVDGA
                     GSAEAVADRVRQLRAEIERTDSEWDRDKLQERLAKLAGGVAVIRVGAATEVELKEKKH
                     RLEDAISATRAAVEEGIIAGGGSALVHAASALDNLDLVGDEATGATIVRKALVEPLRW
                     IAENSGLEGH"
     gene            complement(1544..1855)
                     /gene="groES"
                     /locus_tag="VGD55_08590"
     CDS             complement(1544..1855)
                     /gene="groES"
                     /locus_tag="VGD55_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0870442.1"
                     /translation="MTTATKSKVAIRPLEDRIVVQAVEAETTTASGLVIPDTAKEKPQ
                     EGIVLAIGPGRFEDGQRVPLDVKEGDRVLYSKYGGTEVKYSGEEYLVLSARDVLAVIE
                     K"
     gene            2092..>2346
                     /locus_tag="VGD55_08595"
     CDS             2092..>2346
                     /locus_tag="VGD55_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870443.1"
                     /translation="MDADTFASLATDPGREAISHSIAALDAGRSDLAVGEDVRRRFPA
                     LPNQVASAAVEQATLRRRGRAKFGDKADSMWFTAQGLEQAT"
BASE COUNT          370 a          766 c          781 g          429 t
ORIGIN      
        1 gtggccttcg aggccgctgt tctcggcgat ccaccgaagc ggctcgacga gtgccttgcg
       61 gacgatggtc gccccggtcg cctcgtcgcc gacgaggtcg agattgtcga gagcagaggc
      121 cgcatgcaca agcgcggaac cgccgccggc gatgatgcct tcttcgacag ccgcgcgggt
      181 cgccgaaatc gcgtcctcga gccggtgctt cttctccttg agttcgacct cggtcgccgc
      241 acccacccgg atcaccgcaa cgccgccggc gagcttggcg agccgctcct gcagcttgtc
      301 gcgatcccat tcggagtcgg tgcgctcgat ctcggcgcgc aactggcgca cccggtcagc
      361 gactgcctcg gccgaaccag caccgtcgac gatcgtcgtg tcgtccttgg taatggtcac
      421 ccgacgcgcg gtgccgagta cttccaggcc gacgtcgtcg agcttcagcc cgacctcggg
      481 cgctacgacc tgcgcaccgg tcaggatcgc gatgtcttcg agcatcgact tgcggcggtc
      541 accgaacgcc ggcgccttca ccgcgaccga tgagaacgtg ccgcgaatct tgttgaccac
      601 caaagtcgag agcgcttcgc cgtcgacgtc ctcggcgatg acgagcagcg acttgccggc
      661 ctggatgacc ttctcgagca gcggcagcag gtcggagacc gacgacaact tgccctggtt
      721 gagcaggatc aacgggttct ccaacaccgc ttccatgcgc tcggtgtcgc tgacgaagta
      781 cggcgagatg tagcccttgt cgaactgcag gccctcggtg agctcgagct cgaggcccat
      841 ggtgttcgac tcttcgacgg tgataacgcc gtccttgccg accttgtcga acgcctccgc
      901 gatgaggcca ccgacctctg ggtcttgcgc agaaatcgcg gcgacgtggg cgatctcgcc
      961 cttaccggag acctcacggg cggtgtcgag gagacgcgcg gtcacggcgg tgacggcggc
     1021 ttcgatgcca cgcttcagtg cgagcgggtc ggcgccggcc gcgacgttgc gcaggccttc
     1081 gtggaccatc gcctgcgcga gaacggtggc ggtcgtcgtg ccgtcgccgg ccacatcatt
     1141 ggtcttggtg gcgacttcct tggcgagctg cgcgccgagg ttctcgtggc gatcctggag
     1201 ctcgatctcg cgggcgatcg tgacgccgtc gttggtgatc gtcgggacgc cgtagctctt
     1261 ggcgatgacg acgttgcggc ccttcggccc aagcgtcacc ttcaccgcgt tcgcgagcgc
     1321 gtcaacaccg cgctcaagcg agcggcgggc gctctcgtcg tactgcagga tcttcggcat
     1381 gggcgtggag tcctctcaaa gttcaggcag ttcaggaatt cagatagttc aggcggttca
     1441 ggcatttcat gatgcgggcc gacggacgcc gccccgaggc cttgcggctt cggggcggcg
     1501 gtccatgccc gtgcgagctg gcgcgactga cgccgcgagc gagctacttc tcgatgacgg
     1561 cgaggacatc gcgagcggag agtacgaggt actcctcgcc gctgtacttg acctcggtgc
     1621 cgccgtactt gctgtagaga acgcggtcgc cctctttgac gtcgaggggc acccgctgac
     1681 cgtcttcgaa ccggccgggg ccgatggcga ggacgatgcc ctcctggggc ttctccttgg
     1741 cggtgtctgg gatgaccagg ccggaagcgg tggtggtctc ggcctcaacg gcctgcacga
     1801 cgatgcggtc ctcgagcggc cggatggcta ccttggactt ggtggcggtc gtcacagcgt
     1861 gactccccct cctcagttgt tgtgatgatg gttggtgatg aggttggtct tggttaggcc
     1921 tgccgtcgcg gggtcaggcc tgcggacgtg ttagcacact cgcagttagc aaaccaagta
     1981 cgagagtgcc agcacgtcca tccgaagact atgccgggct tagcactccg tcaacccgag
     2041 tgccaggtat gaatccgctc ccagcacagc cgaccgaatg cgagggaacc gatggacgcc
     2101 gacactttcg cttcgttggc gaccgaccct gggcgcgaag cgatctccca ctcgattgcg
     2161 gccctcgacg caggcaggag cgacttggca gtcggcgaag atgtccggcg acgcttccct
     2221 gcgctaccga atcaggtggc ctccgccgcc gtcgagcagg cgacgctaag acgccgcggc
     2281 cgcgcgaagt tcggcgacaa ggccgacagc atgtggttca ccgcacaggg tctcgagcaa
     2341 gcgacc
//
LOCUS       DASWVF010000482         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_471775, whole genome shotgun sequence.
ACCESSION   DASWVF010000482 DASWVF010000000
VERSION     DASWVF010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471775"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGD55_08600"
     CDS             <1..435
                     /locus_tag="VGD55_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0870444.1"
                     /translation="LPATVVVYDPRVDVAVLLVKGLHAPILTFSQPLATAANAVVAGY
                     PQDGPYTTTAARIRGEEHARGPDIYQDADVTRDIYAIRGVVQPGNSGGPLLDTNGAVA
                     GVVFGKAVNDSTTGYALTASQVSAAANLGRTATLPVSTRGCD"
     gene            complement(555..1007)
                     /locus_tag="VGD55_08605"
     CDS             complement(555..1007)
                     /locus_tag="VGD55_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY0870445.1"
                     /translation="MNDRDRDAAGRARNARPRDGLGRPLPRGSAGGTERVPDKLDLSV
                     DDTIALAQKYLDDGRPFHAHEVLEALWKTQPDAERDLWQGLAQIAVGLTHALRGNAVG
                     AATLLRRGARRVAAYGGQTYGIAVGGVVSWAETLADSPTVLPRPLTLR"
     gene            complement(1009..1935)
                     /locus_tag="VGD55_08610"
     CDS             complement(1009..1935)
                     /locus_tag="VGD55_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0870446.1"
                     /translation="MGAPDAGVMVEGPWTHREISANGMRFHAAEMGATQAPLVILLHG
                     FPQFWWSMRHQLEAVAGAGYRAVAPDLRGYGGSDKPPRGYDAFTLASDVAGMVRALGA
                     RDAIIVGHDWGGYAAWCTAVLHPETVRGIVALAAPHPLRFRNALLTEPRGQLMASAHL
                     VGYQRPWVPERQLVRDDADAVAHMLHAWSTPSWPDEESERRYRDQMQIPHVAHSALEY
                     YRWTLRSQLRRDGQRFNQQLREPVGVPTLQLHGALDTCVLPRTAAGSGRYVGGPYTWR
                     LIDDAGHFLQEESPKAVSAEILHWLDELDHSR"
     gene            complement(1986..2387)
                     /locus_tag="VGD55_08615"
     CDS             complement(1986..2387)
                     /locus_tag="VGD55_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY0870447.1"
                     /translation="MTTPLGERSLGELVATLTRDLALLLHQEIELIKADLVASLKKIA
                     KGAAGFAIAAVLALFALPIISIAIALGVHALGVSLGWSFLIVAGGYLVLAAIAALVGV
                     VMLRRAKPSNRAMTSVKADLHAIARKPGSNR"
     gene            complement(2411..3511)
                     /locus_tag="VGD55_08620"
     CDS             complement(2411..3511)
                     /locus_tag="VGD55_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0870448.1"
                     /translation="MVRVPAPAIVLVAAAIASTYISAVRDAVNLHDIEHVVTGALIVI
                     LFDGGLHLGRRKFRDAAIPIVALGAAGTLATTALIAVAAHVVFGLDWRFAFVLGAALA
                     PTDPAVVFSLLANRTIAGRTPAILEGESGFNDPAGIALLLGFVHLATHPHASVSVMFG
                     TFAIAMLVGCVFGAIAGLGIRWVLHPRRPALNAAQSASALAGIFALYAVADLAHGSGF
                     LAVFVAGIVLDEAAPPTNRVLARIQLNLPRLGELVAFVMLGITIDLRTLPTAHAWLIG
                     LGVFGLLTFVLRPVVCLPLLAVLSRRETAFVAWAGLKGAVPMLLGVLAVSGGVPDASR
                     LLAMVFVVVVASIVVQGGALPWVAGKLGLDRG"
     gene            complement(3703..4107)
                     /locus_tag="VGD55_08625"
     CDS             complement(3703..4107)
                     /locus_tag="VGD55_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870449.1"
                     /translation="MNISGHRISTTEVEHALVGHPSVAEAAVVGANDPITGQAIVAFV
                     TIKGSVVQNEEQAAAFVQELRAHAAKEIGAIAKPRDILITPDLPKTRSGKIMRRLLRD
                     VADHRALGDTTTLLDPAVVSSIASQMPASTEE"
     gene            complement(4104..>4419)
                     /locus_tag="VGD55_08630"
     CDS             complement(4104..>4419)
                     /locus_tag="VGD55_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870450.1"
                     /translation="AVAEAAAVGRPDELKGQALVVFVSLRPAFKGDSDLMIALSEHVA
                     KEIGKFARPDLIRFTDALPKTRSATRRRTGRGSPACTSPATARRRTRTATSGCSGVSM
                     TS"
BASE COUNT          782 a         1483 c         1490 g          664 t
ORIGIN      
        1 ctccccgcga ccgtcgtcgt ctatgacccc cgcgtcgatg ttgcggtgct gctcgtgaag
       61 ggattgcacg cgccgatcct gacgttcagc cagccactgg cgaccgctgc caatgctgtg
      121 gtggcgggct acccccagga cggcccatac acgacgacgg cagcacgcat ccgaggcgag
      181 gaacacgcgc gcgggcccga catctatcaa gacgccgacg tcacccgcga catttacgcg
      241 atccgtggcg tggtgcagcc gggcaactcc ggcggaccgt tgctcgacac caacggcgcc
      301 gttgccgggg tcgtgttcgg caaggcggtc aacgactcga ccaccggata cgccttgacc
      361 gcgtcgcagg tgagcgcagc tgcgaacctc gggcgcacag caacgctgcc cgtctcgacc
      421 cgcggctgcg actagcgcgt cccaaccggc gtcccgtacc tacagttcgc gccccagcgc
      481 gtgatcatgc cgttaggggc gatctagcgg tggtgggctg accctaactg catgatcacg
      541 aagggtgggt cgggctagcg gagggtgagc gggcgtggca gcacggttgg cgaatcggcg
      601 agggtctcag cccaactgac cacgccgccc acggcgatgc cgtacgtctg cccgccgtat
      661 gccgcaacac gacgcgcacc tcgtcggagc aatgtcgcag caccgacggc gttgccgcgc
      721 agtgcgtgcg tcaacccaac agcgatctgc gctagccctt gccacaagtc gcgttcggcg
      781 tcgggctgcg tcttccacag cgcctcgagc acctcgtgcg cgtggaaggg ccggccgtcg
      841 tcgaggtatt tctgcgccaa cgcaatcgtg tcgtcgacgc tcaggtcgag cttgtccggc
      901 acccgttcag tgccgccggc agaaccgcgc ggcagcggtc ggccgagccc gtcgcgcggc
      961 ctcgcgtttc gggcccgccc agccgcgtca cggtcgcgat cgttcacgtc aacgactgtg
     1021 gtcgagttcg tccagccagt gcaggatttc cgccgacacg gccttgggtg actcctcctg
     1081 aaggaaatgc ccggcgtcgt ctatcagccg ccacgtgtaa gggccgccga cgtagcgacc
     1141 cgagcccgcc gccgtccgcg gaagtacgca ggtatcaagg gcaccatgca actgcaaggt
     1201 cgggacgccg acgggctcgc gtagttgttg attgaaccgt tgaccatcgc gccgcaactg
     1261 cgaccgcagg gtccagcggt aatactcaag cgccgagtgc gcgacgtgcg gaatctgcat
     1321 ctggtcgcgg taacgccgct cggattcttc gtcaggccag ctcggcgtcg accatgcatg
     1381 cagcatgtgc gccacggcgt ccgcgtcgtc tcgcactaac tgccgttcgg gaacccacgg
     1441 ccgctgatat ccgacgagat gtgcactggc catcaactgg ccgcgcggtt cggtgagcaa
     1501 tgcgttgcgg aatcgcagcg ggtgcggcgc agcgagcgcc acgattccgc gcaccgtttc
     1561 agggtgcaac acggcggtgc accaagctgc gtaaccaccc cagtcgtgtc cgacgatgat
     1621 cgcgtcgcgc gcaccgagtg cgcgcaccat gccggcgacg tcggaggcca acgtgaaagc
     1681 gtcgtaacca cgcggtggtt tgtcgctgcc gccgtatccg cgaaggtcag gtgcgacggc
     1741 gcggtaacca gcgccggcga ccgcctcgag ctgatggcgc atcgaccacc agaactgcgg
     1801 gaagccgtgc aagaggatga cgagtggggc ctgggtcgca cccatctcgg ctgcgtggaa
     1861 ccgcatgccg ttggcactga tctcgcggtg ggtccacggc ccttcgacca tgacgccagc
     1921 atccggagcg cccataggcc cagcgtgcca cacctggggc cggggagcgc tgggctattt
     1981 gcgggctacc gattcgaccc aggcttgcgg gcgatggcgt gcaggtcggc cttcaccgag
     2041 gtcatggcgc ggttcgacgg cttggccctg cggagcatca cgaccccgac cagcgcggcg
     2101 atcgcggcga gaacgaggta gccgccggcc acgatcagga acgaccatcc caggctgaca
     2161 cccagcgcat ggacgccgag cgcgatggcg atcgagatga tcgggagggc gaagagggcc
     2221 aggacggcgg cgatggcgaa tcccgcggcg cctttggcga tcttcttgag cgacgccacc
     2281 aggtcggcct tgatgagctc gatttcctgg tgcaggagca gggcgaggtc gcgcgtcagg
     2341 gtggccacga gctcacccaa cgagcgctct ccgagcggtg tcgtcatgcg aagagtatga
     2401 ggcacccggg ttatccccgg tcgaggccga gcttgccggc gacccagggc agcgcgccac
     2461 cttgcacgac gatcgatgcg acgaccacca cgaacaccat cgccagcaac ctcgacgcgt
     2521 cgggtacccc cccgctgacg gcaagcacgc ccagcagcat cggtacggcg cccttcaagc
     2581 cggcccatgc cacgaatgcg gtctcgcgcc tgctcaagac cgcgagcagc ggcaggcaaa
     2641 ccactggtct cagcacgaac gtcagcagcc cgaaaacacc gagtccgatg agccacgcgt
     2701 gcgcagtcgg caaggtacgc aggtcgatag tgatgccgag catcacgaag gccaccagct
     2761 cgcctagtcg tggcaggttc agctggatgc gggccagcac ccggttcgtc ggtggcgccg
     2821 cttcgtcgag cacgatgccg gcaacgaaga cggccagaaa tcccgagcca tgcgcgagat
     2881 cggcgacggc gtagagagca aagattccgg cgagcgcgct cgccgactgt gcggcattga
     2941 gagccggacg acgcgggtgc agcacccatc ggatgcccag gcccgcaatg gcgccgaaca
     3001 cgcaccccac cagcatcgcg atcgcgaagg tgccgaacat gacggaaact gacgcatgcg
     3061 gatgcgtcgc gagatgcacg aatcccaaca gcagcgcaat gccggccgga tcgttgaatc
     3121 ccgactcgcc ttcaaggatc gccggtgtgc ggccggcaat cgtgcgattt gccagcaacg
     3181 agaacacgac cgctgggtcg gtaggcgcca acgcggcgcc gagcacgaag gcgaatctcc
     3241 agtcgagacc gaagacgacg tgggcggcaa cagcgatgag ggcggttgta gcgagcgtgc
     3301 ctgctgcccc gagcgcgaca atcggaattg ctgcgtcgcg gaacttgcgg cgaccaagat
     3361 gaaggccgcc gtcgaacaag atgacgatga gcgcgccggt gacaacgtgt tcgatgtcgt
     3421 gcaggttgac ggcgtcgcga accgcgctga tgtaagtgga cgcgatcgct gcggcgacca
     3481 gaacgatggc cggtgccggt acccgaacca tcgatgcacc tcaagtcatg gcaggcgaaa
     3541 cctatgccga ccaggcttcc cggcgcacca gcgcgcagca tatcgccgct gtcggcagcg
     3601 ggccgaggac acgctccggc ccccgaggac gggcgtgtaa gggtgtcctc gggggccgga
     3661 accccctgaa cgtgatcgac tcccgaaacg ggaggccctg cgttactcct cggtcgacgc
     3721 gggcatctgg gacgcgatcg agctcacgac agccggatcg agcagggtcg ttgtgtcacc
     3781 cagcgcgcgg tggtcggcga cgtctcgcag cagccggcgc atgatcttgc ccgaccgcgt
     3841 cttcggcagg tcgggggtga tcaggatgtc gcgcggcttg gcgatcgcgc cgatttcctt
     3901 cgccgcgtgt gcgcgcagtt cttgaacgaa ggcagcggcc tgctcctcgt tctgcacgac
     3961 gctgcccttg atcgtgacga acgcgacgat ggcctgaccg gtgatcggat cgttggcgcc
     4021 gaccacggcc gcttcggcga cgctcgggtg accgaccagc gcgtgctcga cctcggtcgt
     4081 cgagatgcgg tgccccgaga tgttcatgac gtcatcgata cgcccgagca gccagaagtc
     4141 gccgtcctcg tccttcttcg cgccgtcgcc ggcgaagtac aggccgggga accgcgacca
     4201 gtacgtctcc ttgtagcgct ccgggtcttg ggcagcgcgt cggtgaagcg gatcaggtcg
     4261 ggccgcgcga acttgccgat ctccttggcg acgtgctcgg agagcgcgat catcaggtcg
     4321 gagtcgccct tgaacgccgg ccgcagcgag acgaagacca ccagcgcctg ccccttgagc
     4381 tcgtcggggc gccccacggc cgcggcctcg gcgacggcc
//
LOCUS       DASWVF010000483         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_242313, whole genome shotgun sequence.
ACCESSION   DASWVF010000483 DASWVF010000000
VERSION     DASWVF010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242313"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..727
                     /locus_tag="VGD55_08635"
     CDS             200..727
                     /locus_tag="VGD55_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870451.1"
                     /translation="MTAVDAEFAAFVRANERSLLRLAWLLMNDRDAAEDLVQTALEKT
                     MPKWASIRQDDPMAYVHRVMVNTRTSWWRRHRGRESVTSDVVDVGVDDRTDAIAEHDR
                     LTTALRSLTEGQRKVVVLRHYEDMSEAQVAALLGCSTGTVKSQNARGLARLRAALAQP
                     ADQSESALATGTEGA"
     gene            724..1533
                     /locus_tag="VGD55_08640"
     CDS             724..1533
                     /locus_tag="VGD55_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870452.1"
                     /translation="MSDNRDPLVSDALRRLAGAAPAPRIDRLEAVVAGVRHRRQVRAT
                     TSTLAAAALVVAAAVGVHSWHTTASDQTVGPVPVTSSPGVAPSSPAASPAASATSILS
                     ATPAPSATTRPTSSKGLVETVTVPASVTVGEQARAVMTVINKGAVATHGGAFTLVNAY
                     AENGTQATGTVSGGHSVGCVAAAPGLRCSVATLEPGHSVQFWVSVAASTQATAIVFDI
                     RWQYTGPDGATTQTPFPRSVKVAALPSSQPPSSEVPVSSEVPTGSPSATSS"
     gene            1574..1894
                     /locus_tag="VGD55_08645"
     CDS             1574..1894
                     /locus_tag="VGD55_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870453.1"
                     /translation="MSPRRTRPTRRSASPGVTVAADEDGDTGLTGGFQFVEVDARGEE
                     WIVRRLTGVSSTKTYRCPGCNQEIRPTTPHVVVWPSLAVDMRRHWHTPCWNSRSRRSS
                     RELR"
     gene            complement(1910..>2910)
                     /locus_tag="VGD55_08650"
     CDS             complement(1910..>2910)
                     /locus_tag="VGD55_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870454.1"
                     /translation="LEFYTFAGIACFVLAVFFYRCRYAVMKRAWVPLVGASFFLPTRS
                     FGSYLFMLVPAAIIAGVTAGPVQISWRPIPRRWLEPALLTGAVLTVGLASIAALSPSP
                     LSIRVIDTQSTGQLGTIDQATVQVSNRTGHTQHPHFTVETQQHATTFWNPVDASGQPV
                     SPTIAPHSQATFILRAPNTGSMPPVAAPMFIAAFTGSPSAMSTSDSYLVSRISTEIIP
                     DAVNSSIPVGQPIALAVQLQDQYGNPVHKAGLTVLLGQVVYAYGGLLAGESSINGQGE
                     GSSPVPANTDANGIAHFTVIGDEVQPQPVFYEAWIADSSTVPHGYSNQVSIQFRAR"
BASE COUNT          504 a          917 c         1007 g          482 t
ORIGIN      
        1 gcgcggccga gcgcgcgtgg cagtacctcg cctgagggcc atacctgtac ttgtcgggga
       61 gcacgacggg gttctgtagg ttcttcttga tccaatctgc cgctcgtgac ttcccgctcg
      121 gctcgccgcg tcgtcctagg ctgacaacct cgcgacgatc gcgtgcgtct accggggcga
      181 ggcaggggga ggaggcgcgg tgacggcagt cgacgccgaa ttcgccgcct tcgtgcgagc
      241 gaacgaacgg tcgttgctgc gcctcgcctg gttgctgatg aacgatcgtg acgccgccga
      301 agatctggtg cagacagcac tcgaaaagac gatgccgaaa tgggcatcga tccgccagga
      361 cgatccgatg gcgtacgtgc accgggtgat ggtcaacacg cggacgtcgt ggtggcgccg
      421 gcatcgcggc cgcgaatcgg tgacgagcga tgtcgttgac gtcggtgttg acgatcgaac
      481 agacgcgatt gccgagcacg acaggctgac cacggcgttg cgcagcctga cggagggcca
      541 acgcaaagtg gttgtccttc gccattacga ggacatgtcg gaggcgcagg tcgcggcgct
      601 gctcggctgc tcgacaggaa cggtaaagag ccagaacgcg cgcggactag cccgactacg
      661 tgcggcactc gcacagccgg cggatcagtc cgaatctgct ctcgccactg ggacggaggg
      721 cgcatgagcg acaatcgcga tccattggtt tcggacgcac tgcgccggct cgcaggtgcg
      781 gcacctgcgc cacgaatcga ccggctcgag gctgtcgtcg ccggcgtgcg ccaccgtcgg
      841 caggtgcgcg caaccacgag caccctggcc gcggccgctt tggtggtggc ggccgcggtc
      901 ggggtccact catggcacac cacggcgtcc gaccagaccg tcggtccggt gccggtgacg
      961 tcgagccccg gtgtggcgcc gagcagcccc gcggcctcgc ctgcggcgtc cgccacgtcg
     1021 atcttgagcg caacgcccgc gccgtcagcg accacccggc cgacgtcgtc gaaaggcctc
     1081 gtggagaccg tgaccgtgcc ggcatctgtg acggtgggcg aacaggcacg ggcggtcatg
     1141 actgtgatta acaagggagc ggtggccacc cacggaggtg cgttcaccct cgtcaacgcc
     1201 tacgccgaga acggcacgca ggcgaccgga accgtgtcag gcggccattc ggttggatgc
     1261 gtcgcggcgg ctcctggcct gcggtgctct gtggcgacct tggagccggg ccacagcgtg
     1321 cagttctggg tgtcggtcgc ggcctcgacg caggcgaccg cgatcgtgtt cgacattcgg
     1381 tggcagtaca ccggccctga tggcgcgacc acacagaccc cgttcccgcg ttcggtcaag
     1441 gtggcggcat tgccgtcgtc gcaaccgccg tcgagtgagg tgccggtatc gagcgaggtg
     1501 ccaacaggtt cgccgagcgc gacctccagc taggttccga ccgcgctgcc accccgcgga
     1561 tacgctggtt tacatgagcc cgcgtcgcac ccgacccacc cgcaggtcgg catctccggg
     1621 agtgactgtc gctgccgacg aggacggcga caccggactc acgggcggtt ttcagttcgt
     1681 cgaggtcgac gcgcgcggtg aagagtggat cgtccgccgg ttgaccggtg tctcttcgac
     1741 caagacctac cgctgcccag ggtgcaatca ggagatccga cccacgacgc cgcacgtcgt
     1801 cgtgtggccg agcctggcag tcgacatgcg ccggcattgg cacacgccgt gttggaactc
     1861 gcggtcgcgc cggtcatcgc gcgaactgcg ctagctgccg ggcaggttgt caacgcgccc
     1921 tgaactgaat ggagacctgg ttcgagtacc cgtgcggcac ggtgctgcta tcggcgatcc
     1981 acgcttcgta gaacaccggt tgcggctgca cttcgtcgcc gatgaccgtg aagtgcgcga
     2041 tgccgttggc gtcggtgttc gcgggcaccg ggctcgagcc ttcgccttgc ccgttgatgc
     2101 tcgactcgcc ggccaggagc ccaccgtagg cgtacacgac ctgaccgagc agcacggtga
     2161 ggccagcctt gtgcaccgga ttgccgtact gatcttgcag ttgcaccgcc aacgcgatcg
     2221 gctggcctac gggaatcgac gagttcacgg cgtccggaat gatctctgtc gagatccgtg
     2281 acacgaggta ggagtcgctc gtcgacatcg ctgacgggct gccggtgaag gccgcgatga
     2341 acatcggcgc tgctactgga ggcatcgacc cagtgtttgg cgcgcgcaag atgaacgttg
     2401 cttgactatg cggagcgatc gtcggactaa ccggctgacc tgaggcatcg accgggttcc
     2461 agaaggtggt cgcatgttgc tgcgtctcga cggtgaagtg cggatgttgg gtgtgtccgg
     2521 tgcggttgga gacctgcacg gtcgcctggt cgatggtgcc gagctggccg gttgattgag
     2581 tgtcgatgac tcgaatagag agcggggacg gcgacagcgc ggcgatcgat gccaggccca
     2641 ccgttagtac agcgccagtc agtaaagccg gctcgagcca acggcgcggt atcggccgcc
     2701 agctgatctg cactggacca gcggtcacac cggcgatgat ggcggcgggt accagcatga
     2761 acaggtaaga accgaacgac cgtgtgggta agaagaatga cgcccctacc aacggaaccc
     2821 acgcgcgctt catgaccgcg tagcgacacc ggtagaagaa caccgccagg acgaaacagg
     2881 cgatgccggc gaaggtatag aactccagcc
//
LOCUS       DASWVF010000484         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_318425, whole genome shotgun sequence.
ACCESSION   DASWVF010000484 DASWVF010000000
VERSION     DASWVF010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318425"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..2048)
                     /gene="ilvD"
                     /locus_tag="VGD55_08655"
     CDS             complement(357..2048)
                     /gene="ilvD"
                     /locus_tag="VGD55_08655"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0870455.1"
                     /translation="MTTNHEPDIKPRSRVVTDGLEASASRGMLRAVGMGDDDWVKPQI
                     GVASSWNEITPCNLSLDRLAKSAKEGVHAAGGYPLEFGTISVSDGISMGHEGMHFSLV
                     SREVIADSVETVMMAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGTTLAGKI
                     GDRDNITIIDAFEAVGGCVRGLVTREEVDAIERVFCQVEGACAGMYTANTMACAAEAL
                     GMSMPGSAAPPAPDRRRDGFARASGEAVVGLLHKGITARQIMTKSAFDNAIAVVMALG
                     GSTNAVLHLLAIAREADVDLDLDDFNRIGDRVPHLADVKPFGRYVMSDVDHVGGVPVV
                     MTALLDAGLIDGSALTVTGRSVAENLADLAPSDPDGKVIHAMTDPLHRTGGLTILRGS
                     LAPDGAVVKTAGIDRAGFEGTARVFDGERAAMDAVENGALQAGDVVVIRNEGPKGGPG
                     MREMLAVTGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEAVDGGPIAFVRDGDR
                     IKLDVTTRTLDLLVDADELAQRRIGWEPLPPKYKTGVLSKYRKLVGSAAYGAVCS"
     gene            complement(2076..>2765)
                     /locus_tag="VGD55_08660"
     CDS             complement(2076..>2765)
                     /locus_tag="VGD55_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870456.1"
                     /translation="AVKDMAARTSASSFVVDVSVGTAEFTVKDAPDIQLLPRFVGSIT
                     SNGPPAASPLPTPSVERGQPDPALVTEALQVAATWEKSGPVVNAHPVVVWGGTDVAGT
                     KLVVLRVDTQIVDLIVLEWSGDAPGLHGEILIHSTAPEVPVAFAYRALDGTRIGVIGS
                     YGAVRAVLHYNGKVSAPVALADGFASFKVTNPSPPPSNDAASDLEIAASVQLFDASGQ
                     VIAAVPEPPSV"
BASE COUNT          483 a          956 c          885 g          441 t
ORIGIN      
        1 tctccatctc aacgcacctc tggacaacgc ggtaactctg gacaacgcgg tgactctgga
       61 caagaaaaag ccccctgtgc cgtgtggcac aaaggggcag cgcgctagct ggaccggttg
      121 gtcagcttgc gcgcttgtct actacaagaa tcttcacacc ctcagaatcg cccacggatc
      181 ggccggtgtc aaccgaccaa cgcgggcgag gcgctcagaa acccttagat acaaggactt
      241 cctactactt tgcggcagtg ctcagtctcc gggtcggcga gggggcggaa ggttggggtc
      301 gctagctgca gacggctctc acacgggccg cctgccttcc gctactcgct ccgctcctag
      361 ctgcagacgg cgccgtaggc cgcggacccg accagcttgc ggtacttcga cagcaccccg
      421 gtcttgtact tcggcgggag aggttcccaa ccgatccgtc gctgcgccag ctcgtcggcg
      481 tccaccaaga gatcgagcgt gcgggtcgtg acgtcgagct tgatgcggtc gccgtcgcgc
      541 acgaacgcga tcgggccgcc atcgactgcc tcgggagcga cgtggccgac gcagagcccg
      601 gtggtgccgc cggagaagcg gccgtcggtc agcaacagca cgtccttgcc gaggccggcg
      661 cctttgatcg cgccggtgac cgcgagcatc tcgcgcatgc ccgggccgcc tttggggcct
      721 tcgttgcgga tcacgacgac gtcgccggcc tgcaacgcac cgttctcgac ggcgtccatc
      781 gcagcccgct cgccatcgaa cacccgtgca gtgccttcga agccagcgcg gtcgatacct
      841 gcggtcttca cgacagcacc gtcgggtgcc aacgatccgc gcaggatcgt caacccgccg
      901 gtgcggtgga gcgggtcggt catcgcgtgg atcaccttgc cgtcggggtc ggacggcgcg
      961 aggtcggcga gattctcagc gaccgaccga ccagtgaccg tgagagccga cccgtcgatg
     1021 agcccggcgt cgagcaatgc cgtcatgacg accggaacac cgcccacgtg gtcgacgtca
     1081 ctcatgacgt agcggccgaa cggcttgacg tcggccaggt gcggaacgcg gtcgccgatc
     1141 cggttgaagt cgtcgagatc gaggtcgacg tcggcctccc gcgcgattgc aagcaggtgc
     1201 aaaacagcgt tggtcgagcc gccgagtgcc atgacgacgg cgatcgcgtt atcgaacgcg
     1261 gacttcgtca tgatctgccg cgccgtaatt cccttgtgca gcaggccgac cacggcctca
     1321 cccgacgcac gcgcgaaccc gtcgcgccga cggtcgggcg cgggcggagc tgccgagcca
     1381 ggcatcgaca tgccgagcgc ctcagcggcg caggccatcg tgttcgccgt gtacatgccg
     1441 gcgcaggcgc cctcaacttg gcagaacacc cgctcgatcg cgtcgacctc ctcgcgggtg
     1501 accagcccgc gcacgcaccc acccacggcc tcgaacgcat cgatgatcgt gatgttgtcg
     1561 cgatcgccga tcttgccggc caacgtggtg ccggcgtaaa ggaacaccga ggcgaggtcg
     1621 aggcgggcgg cggccatcaa catgccgggc agcgatttgt cgcacccggc cagcaacacc
     1681 gagccgtcga gccgctccgc catcatgacg gtctcgaccg agtcggcgat gacttcgcgc
     1741 gacaccagcg agaagtgcat gccctcgtga cccatcgaga ttccgtcgga caccgagatc
     1801 gtgccgaact cgagcggata tccgccggcc gcgtgcacgc cctctttcgc cgacttcgcc
     1861 agccggtcga gcgagaggtt gcacggggtg atctcgttcc acgaactggc gacgccgatc
     1921 tgcggcttca cccagtcgtc gtcacccatc ccgaccgcgc gcaacatgcc ccgcgacgcg
     1981 gacgcctcga ggccgtcggt cacaacgcga ctgcgtggct tgatatctgg ttcgtgattc
     2041 gtcgtcatga ggcgtcacat tacgtgaacg acacgtcaga cggacggcgg ctcggggacc
     2101 gccgcaatca cctgcccgct cgcgtcgaac aactgcaccg atgccgcgat ctccaagtcg
     2161 ctcgcggcgt cgttgctcgg cgggggagac gggttcgtca ccttgaagct ggcgaatccg
     2221 tcagcaagag caacaggagc tgaaactttg ccgttgtaat gcaggaccgc gcgtacggca
     2281 ccgtacgagc cgatcacgcc gatccgggtg ccgtcgaggg cgcggtaggc gaaggccacc
     2341 ggcacctcag gtgccgtgga atgaataagt atttcgccgt gcagcccggg ggcgtcgccg
     2401 ctccactcca gaacgatcag gtcaacgatc tgggtgtcaa cccgcagcac aaccaacttc
     2461 gtgcccgcaa cgtcagtgcc gccccacacc accaccgggt gcgcattgac caccggcccc
     2521 gacttttccc acgtggcggc aacctgtagc gcctcggtca cgagcgcggg gtcgggctgt
     2581 ccacgttcga cgctcggcgt cggtagcggc gacgccgccg gcggcccgtt actcgtgatc
     2641 gaacccacga atcgcggcag gagttggatg tcgggcgcgt ccttgaccgt gaactccgcg
     2701 gtcccgacgc tgacatcgac aacgaagctg gacgccgagg tgcgcgctgc catgtccttc
     2761 accgc
//
LOCUS       DASWVF010000485         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_191425, whole genome shotgun sequence.
ACCESSION   DASWVF010000485 DASWVF010000000
VERSION     DASWVF010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191425"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGD55_08665"
     CDS             <1..475
                     /locus_tag="VGD55_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870457.1"
                     /translation="ALFYGRRAVHAAGVEHPWWQIAANSILATTLLEAGETAQAVELL
                     ETARAVAQQDGSQAYRLRCLAPLAEATGARDVLIEATALLAQISAPAGSAWFWGGDAY
                     LSVARAWLAAGEPAQARLALAPLLDAASRVPWSSTLDVALLLDARAAVAAGSMST"
     gene            complement(480..2600)
                     /locus_tag="VGD55_08670"
     CDS             complement(480..2600)
                     /locus_tag="VGD55_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0870458.1"
                     /translation="MRARYPDLEGFVERDGVKIGYEVFGDGDPTILLLPTWSIVHSRF
                     WKHQVAYLARHYRVITYDGPGNGRSDRPTDPAAYAASAIVDDAVRVLDAVGAKAAIAV
                     GFSLGGGFLLELAARYPQRMLGAVFVAPTVGLSDPHDRPNYPFDDELDTDEGWAKYNA
                     QYWLRDWPGFAEFFMGRVFTEPHSTKAIEDCVGWALETTPEAIIATQRAPYLEYAGDA
                     DDDRAAVYKLADLVSCPCIVVEGSQDDVIGVGGGRRLASALHCEYVEFEGSGHLPAGR
                     DPVHFNLLLRAFVDRVQPPARVTSWSRARSRRPRALYLSSPIGLGHALRDVAIASELR
                     ALQPDIEIDWLAQHPVTTVLQKNGEQVHPASQWLASESAHIESESAEHDLHCFQAFRR
                     MDEILVANFHVLDDVLSAGHYDLVVGDEAWDADFYWHENPELKRTAFAWLTDFVGWLP
                     MPAGGEREAFLTADYNAEMIEQVERYPRVRDRAIFVGHPEDIVPDLFGPGLPSIREWT
                     QEHYEFSGYITGFDPAALGERMALREQLGYHPDEQVCIVTVGGSGVGAALLRRVIAAF
                     PIARQAMPNLRVIVVAGPRIEPASLPSHPGLEIRPYVHDLYKHLAACDLAVVQGGLTT
                     CMELTALRRPFLYFPLRNHFEQNFHVRQRLSRYGAGLCMDYATTDVEAMAAAITSEIG
                     KPVTYEAVETDGAAVAARRIASLV"
BASE COUNT          439 a          860 c          908 g          467 t
ORIGIN      
        1 ggctttgttc tacggacgcc gagcggtgca cgcggcggga gtcgaacatc cttggtggca
       61 gattgccgca aactcgatac tcgcgacgac attgctcgag gccggcgaaa cggcacaagc
      121 agtcgagttg ctcgagacgg cgcgcgcggt cgcacagcag gacggctcgc aggcgtatcg
      181 gctgcgctgc ttggcgcccc ttgccgaggc aaccggcgcg cgcgacgtgt tgatcgaagc
      241 gacggcactg ctcgcgcaga tctcggcgcc cgctggttct gcatggttct gggggggtga
      301 cgcttatttg tccgtcgcgc gcgcatggct agctgccggt gagccggcgc aggcccgtct
      361 tgcattggcg ccgctgctcg acgccgcgtc gcgagtgcca tggtcgtcga cgctcgacgt
      421 cgcgctgctg ctcgacgcga gagcagccgt cgccgcaggc tcgatgtcga cctgatggtt
      481 tacaccagcg aggcgatccg ccgcgcggcc accgccgcgc cgtcggtttc gactgcttcg
      541 taggtcacgg gtttgccgat ttccgaggtg atcgccgcgg ccatcgcctc gacgtcggtg
      601 gtcgcgtagt ccatgcacag acctgccccg tagcgcgaga gccgctgccg cacatggaag
      661 ttctgctcga agtgattccg gagcgggaaa tacaggaacg gccgtcgcag cgctgtcaac
      721 tccatgcacg tcgtcaatcc gccctgcacg acggcaaggt cacacgcggc tagatgcttg
      781 tagaggtcgt gaacgtaggg tcggatctcc aagcctggat gcgacggcag cgacgcgggc
      841 tcgatgcgcg gaccggccac gacgatcacg cgcaggttcg gcatcgcctg acgcgcgatc
      901 gggaacgcgg cgatgactcg gcgcaacagc gcggctccga caccgctgcc gccgaccgtg
      961 acgatgcaga cctgctcgtc cgggtggtag ccgagttgtt cgcgaagtgc catgcgttca
     1021 ccgagcgcgg ctggatcgaa gccggtgatg tagccggaga actcgtagtg ttcctgcgtc
     1081 cattcgcgga tcgacggcaa ccctggcccg aagaggtctg gaacgatgtc ttcggggtgt
     1141 ccgacgaaga tggcccgatc ccgcacgcgt gggtagcgct cgacctgctc aatcatttcg
     1201 gcgttgtagt cggcggtcag gaaggcttcg cgctcaccgc ccgccggcat cggcagccag
     1261 ccgacgaagt cggttaacca cgcgaacgca gttcgtttga gttcaggatt ctcgtgccag
     1321 tagaagtcgg cgtcccacgc ttcgtcaccg acgacgaggt cgtagtgccc ggcgctcaag
     1381 acgtcgtcaa gcacgtgaaa gttcgcgacg aggatctcgt ccattcggcg gaacgcctga
     1441 aaacagtgca ggtcgtgttc ggcggactcg ctctcgatgt gcgcggattc actggccagc
     1501 cactgcgatg ccggatgcac ctgctcgccg ttcttctgca acacggtggt gacgggatgt
     1561 tgtgcgagcc agtcgatctc gatgtcgggt tgcagggcgc ggagctcgct cgcgatggcg
     1621 acgtcgcgca acgcatggcc gaggccgatc ggcgacgaca agtacaaggc gcgcggacgc
     1681 cgtgacctcg cgcgcgacca cgacgtgact cgcgccggtg gctgcacgcg atcgacgaag
     1741 gcgcgaagga gcaggttgaa gtgcacgggg tcgcgacccg ccgggagatg acccgaccct
     1801 tcgaactcga cgtactcgca gtgcaacgcc gacgcgagcc gtcgcccgcc gccgactccg
     1861 atcacatcgt cttgcgatcc ctcgacgacg atgcacgggc agctgaccag gtcggcaagc
     1921 ttgtagaccg cggcgcggtc gtcgtctgca tcgccggcgt actcgaggta gggcgctcgc
     1981 tgggtggcga tgatcgcctc gggtgtggtc tccagtgccc acccgacgca gtcttcgatc
     2041 gccttcgtcg agtgcggttc ggtgaatacc cggcccatga agaactcggc gaagcccggc
     2101 cagtcgcgca gccagtactg cgcgttgtac ttcgcccagc cctcgtcggt gtcgagctcg
     2161 tcgtcgaatg ggtagttcgg ccggtcgtgc gggtcactca gcccgaccgt gggtgcgacg
     2221 aacaccgcac cgagcattcg ctgcgggtaa cgtgcggcca actcgaggag gaaaccgcca
     2281 cccaaggaga agccgacggc gatggctgcc ttcgcgccga ccgcgtcgag caccctgacg
     2341 gcgtcgtcga cgatcgcact cgccgcgtac gcggcagggt cggtcgggcg atcactgcgg
     2401 ccgttgccgg ggccgtcata ggtgatgacc cggtagtgcc gggcgagata ggcgacctgg
     2461 tgcttccaga aacgcgaatg aacgatcgac cacgtcggca gcaacaggat ggtcgggtcg
     2521 ccgtcgccga agacctcgta gccgatcttc acaccgtcgc gttcgacgaa cccctcgagg
     2581 tctggatatc tggctctcat ctcggcctcc ctgtcgctgt cctgtccttg gctgttgccg
     2641 ggagcgtgcc gcgtactcat tgcagggccg ttgc
//
LOCUS       DASWVF010000486         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_446460, whole genome shotgun sequence.
ACCESSION   DASWVF010000486 DASWVF010000000
VERSION     DASWVF010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446460"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1147
                     /locus_tag="VGD55_08675"
     CDS             209..1147
                     /locus_tag="VGD55_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY0870459.1"
                     /translation="MTNASAVTGGHRPPSGLQFRLTFDDQIATVVEVGAAVREFRVGD
                     RDVFQSYAESAFSTGFHGAVLVPWPNRLADGRYVFDGDEYQVAISEPSRMTALHGLSP
                     WRPWSVVEHEADRVSLRLSLLPSPGYPFYLDTTVDYRLGADGLRVTTTSRNVGDRACP
                     YAVGFHPWLSSGDATLDECTLSLDATHRFVTDDRLLPMGEEPVAGTPYDFRTQRLLGA
                     LVLDDAFTGAPRAADGRAWVRLGGPDGRTAAVWMDDSCDYWQLCTGDMLPAHQARRGL
                     AAEPMTAAPNAFVTGRGLRRLEPGEHVTTTWGACLL"
     gene            complement(1165..1830)
                     /locus_tag="VGD55_08680"
     CDS             complement(1165..1830)
                     /locus_tag="VGD55_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870460.1"
                     /translation="MTGIRMTPGTKGFGVAIGCLALALMVTACAGSKSKTASASSSAP
                     AVSPSVSAAPSSAPASGGARNTAAFAAYTACLTQHGVTVPSFSRGPRPSGSFSRPPGS
                     FSRPPGASRGPGGGGFGGFGGSADPSTAAARAACASLLPAGAFGGGGGRTISATTFAA
                     FKSCMSDNGVTITVTDPTQAIRSLNRSDAKTAAALKICQPILGTQGTQGSGAPSPTPA
                     ASS"
     gene            complement(1830..>2291)
                     /locus_tag="VGD55_08685"
     CDS             complement(1830..>2291)
                     /locus_tag="VGD55_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870461.1"
                     /translation="AGVGTGGTAAARPSGAAATGGTAATGGTGATGAAGTGGVATGGA
                     ATTGGAANIVGTVTVVDGTTLYVTDASGNTVKVTTNTGTTVTKTDTGTVKDLAPGETV
                     VIRGVQSSDGVYAAQTVSEGAAGAFAGGFGGRGGRGGAAVPSASASASAGN"
BASE COUNT          347 a          822 c          752 g          370 t
ORIGIN      
        1 gtgcacatag cggcccggat ggttcaggtt gatcgccgac agtcgcgagg ctgccgagag
       61 ggctgagcgc atcgttcctc ctgcgagagt tcccgtcccc taagggcacg atggggtagg
      121 caactcggcg tcagaaagag cagaaaggca cccgccggag ggaccttcgg caacgccacg
      181 ccgatggccc gcgctaacct ctcagccgat gacgaacgct tcggctgtaa cgggcggcca
      241 ccgaccgccg tccggccttc agtttcgact gacattcgac gaccagatcg cgaccgtcgt
      301 cgaagtcggt gccgccgtca gggagttccg ggtcggcgac cgtgacgtct tccagtccta
      361 cgcagagtcg gcgttctcga ccggcttcca cggagcggtg ctcgtgccgt ggccgaaccg
      421 gctcgccgat gggcgttacg tgttcgacgg cgacgaatat caggtggcga tttctgagcc
      481 gtcacggatg accgccttgc acggcttgtc gccgtggcga ccctggagcg tcgtcgagca
      541 cgaggccgac cgggtcagcc ttcgactgtc gctcctcccc agccctgggt acccgttcta
      601 cctcgacacc acggtcgact accggctcgg tgccgacggc ctgcgcgtaa cgacgacgtc
      661 gcgcaacgtc ggtgaccgcg cgtgccccta cgccgtcgga ttccatccct ggctctcgtc
      721 aggtgacgca acccttgacg aatgcacgct gagtctcgac gccacacacc ggttcgtcac
      781 cgacgaccgg ctgctgccca tgggcgaaga gcccgtcgcg ggcacgccgt acgacttccg
      841 cacacagcgc ttgctcggcg cgctggttct cgacgacgcg ttcacgggag caccacgcgc
      901 cgccgacggc agggcctggg tgcgactggg cggccccgac gggcgcactg ccgcggtctg
      961 gatggacgat tcgtgcgact actggcaact gtgcaccggc gacatgctgc ccgcccatca
     1021 ggcacgtcga gggctcgcgg ctgagccgat gaccgctgca ccgaacgcgt tcgtgaccgg
     1081 acgcggcctt cgacgcctcg agcctggtga gcacgtcacg acgacgtggg gcgcctgcct
     1141 gctgtaggcg ccccacgcga cacgtcagct agacgcggcg ggtgtcgggc tcggtgcacc
     1201 cgagccctgc gtgccctgcg tccccagaat cggctggcag atcttcagtg ccgccgcggt
     1261 cttcgcgtcg ctgcggttga gcgaccggat cgcctgcgtc ggatcggtga cagtgatcgt
     1321 cacgccgttg tcagacatgc agctcttgaa cgcggcaaac gtggtggcgc tgatagtccg
     1381 acctccaccg ccaccgaacg ctccggctgg cagcaggctt gcacatgccg cgcgagcggc
     1441 tgctgtactg ggatccgcac tgccaccaaa cccaccgaag ccaccgccgc ccggaccgcg
     1501 gctcgctccc ggcggccgac tgaagctgcc gggcggccgg ctgaaactgc ctgaggggcg
     1561 aggtccgcgc gagaacgacg gcaccgtcac cccgtgttgt gtgaggcacg ccgtgtacgc
     1621 ggcgaatgcg gccgtattgc gggcgccgcc actcgccggc gcgctgctcg gcgcggcact
     1681 aacagaagga ctcactgcgg gggcggacga cgaggcgctg gcagtcttcg acttgctgcc
     1741 ggcgcaagcc gtcaccataa gagcgagtgc gaggcaccca atggcaacgc cgaacccttt
     1801 cgtgcccgga gtcatgcgga tgccggtcat cagttgcccg ccgatgctga cgcagacgcg
     1861 cttggaactg ccgctccacc gcggccgccg cgcccaccga acccgccggc gaacgcgccc
     1921 gccgcaccct cgctcaccgt ctgcgcggcg tacacgccgt cagacgattg cactcctctg
     1981 atcaccactg tctcccctgg cgcgagatct ttcacggtcc ctgtgtcggt cttggtgact
     2041 gttgtcccgg tgttggttgt caccttcacc gtgttgcccg atgcgtcggt gacgtacaac
     2101 gtggtgccat cgacgacggt gacggtgcca acgatgtttg cggcaccgcc cgtcgtggca
     2161 gcaccacctg tcgccactcc gccagtgccc gcggctccgg tagcgccagt gcccccggta
     2221 gcggcagtgc ccccggtagc cgccgcgcca ctcggacgtg cggctgcggt gccgccggta
     2281 ccgaccccgg c
//
LOCUS       DASWVF010000487         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_369654, whole genome shotgun sequence.
ACCESSION   DASWVF010000487 DASWVF010000000
VERSION     DASWVF010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369654"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1137
                     /locus_tag="VGD55_08690"
     CDS             46..1137
                     /locus_tag="VGD55_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0870462.1"
                     /translation="MKANLGAIGLAVAATLGLGFSLTGADSDAAPRTATSASASASSP
                     PSPAALPAPAASSLPFVASSGQAVQLSFSSPGVLAQVLVKVGDRVTKGQALATLDASA
                     ATIAVTSAKANADKAKIALAALTQGLSAQEQAQIAQGEAQAQAAVANAQQALSDAQSE
                     ADQDAETQASLLSAAQQSLTDTQAQASLDATDAAQQVAAAQSQLATDQAQTPPDQVKI
                     AADTAAVTAAQNSQASLALKDTQQVHMATASVTNAENSAVATALRDAQSIHQAQAAVD
                     AQQQQLAATKAADAVHTEPPRPTALENARDDVTSAQAQLQSAELALAATTLRAPAAGV
                     IAAINGLVGESLAGSGATGFITLEEPGSS"
     gene            1134..2234
                     /locus_tag="VGD55_08695"
     CDS             1134..2234
                     /locus_tag="VGD55_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY0870463.1"
                     /translation="MTRLIPRRIVVVIVVVVVAVGLVIGGVALFAKKTPAQAAGADNT
                     AATSTVAVANRSLSAQTNVSATLGYAGIYSVVNQALGSFSRLPKVGDVIEPGSLLYAV
                     GGQPVVLLTGSTPAYRTLVAGISGPDAQELNAALVALGYGSAATLQGSTVFNAATAAA
                     VVQLQARLGVAQTGVLASDQVIFLPSAARITAVSAALGATAQEGEVVATATSTTRQVV
                     VALDATQQAAVKVGDQVTITLPDTSTTPGVVSTVGTVATAPAGNGQGQSGTPTIEVDV
                     TPTDPTATGTLDQAPVLVAITTASVTDALVVPVPALLALSGGGYAVEVVAADGTHQLV
                     GVTLGLFDDAAGLVQINGSSVRAGQHVVVPSS"
     gene            2231..2947
                     /locus_tag="VGD55_08700"
     CDS             2231..2947
                     /locus_tag="VGD55_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870464.1"
                     /translation="MTVVLELDDVTKVYPGQPPVTALDGVSFSVSAGELVGVVGPSGS
                     GKTTLLQMIGTLDRPTSGTVHITDMDVAVLSDRELSRLRAGKIGFVFQQFFLAEHATL
                     LDNVADGLLYAGVALRDRRAAALIALEQVGLADRVTARPTQLSGGQRQRVAIARALVG
                     RPAIVLADEPTGNLDTATGQAILALIEQLHAGGTTIVVVTHDRDIAARMPRQIEMRDG
                     HIIADTAFSASGQLSTPSRS"
     gene            2944..>3405
                     /locus_tag="VGD55_08705"
     CDS             2944..>3405
                     /locus_tag="VGD55_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870465.1"
                     /translation="MTTAVPPRSSRRLRLADLARLSTIGLRTRKLRASLSALGVAIGV
                     AAVVAVLGLSASSQAGLLAEIDKLGTNLLTVTNGQTLFGQAAELPDAAPGMISRIGPV
                     ITVQATGAVTASVYRSPLIPAIQTNALSVLASTLDLPATVGTTLATGRYLNA"
BASE COUNT          595 a         1118 c         1124 g          568 t
ORIGIN      
        1 tcaggcgtcg gcgggtagtg gcggcccgcc agggagcagc agcccgtgaa agccaacctg
       61 ggcgcgatcg gtcttgcggt cgcggcgacc ctcgggctcg ggttctcgct gacgggcgcc
      121 gacagtgatg cggcgccacg aactgcgacg agtgcatcgg ccagcgcctc atcgccgccg
      181 tcaccggctg cactacccgc accggcggcg tccagcctgc cgtttgtcgc gagttcgggg
      241 caggccgtgc aactgagttt ctcgtcgccc ggagtgctgg ctcaggtgct ggtcaaggtc
      301 ggcgatcgcg tgacgaaagg tcaggcgttg gcgactctcg atgccagcgc ggcgacgatc
      361 gcggtgacca gcgcgaaagc caacgcagac aaggcgaaaa tcgcgctagc cgcgttgacg
      421 cagggattgt cggcgcagga gcaggcgcag atcgcccaag gtgaggcgca agcacaagcc
      481 gcagtagcca acgcgcagca agcgttgtct gacgcgcaat ccgaagctga tcaggacgcc
      541 gaaacgcagg cgagtctgct cagcgcggcc cagcagtcgt tgaccgacac gcaggcgcag
      601 gcgagcctcg acgcgaccga tgccgcgcag caggtcgccg ccgcgcagtc acagcttgcg
      661 accgatcaag ctcagacccc gcccgatcaa gtgaagatcg ccgcagacac ggcggctgtg
      721 acagccgcgc aaaacagcca ggcaagcttg gcgttgaagg acacccagca ggtgcacatg
      781 gcgaccgcga gcgtgacgaa tgcggagaac agcgccgtgg caaccgcgtt gcgtgatgcg
      841 cagtcgattc accaggcgca agccgccgtc gatgcgcagc agcagcagct tgcggcaaca
      901 aaggcggccg atgcggtgca cacggaaccg ccgaggccca cggcgctgga aaacgcgcgc
      961 gacgacgtca cgtctgcaca agcacaactg cagtcggctg agctggctct ggcggcgacg
     1021 acgcttcgcg ctccagcggc cggtgtgatt gcggcaatca acggtctcgt cggtgagtcg
     1081 ctcgcaggca gcggcgctac cgggttcatc actctggaag agcccggcag ttcatgacac
     1141 ggctcattcc acggcgcatc gttgtcgtga tcgtggtggt cgtcgtcgcc gttggtttgg
     1201 tgatcggtgg tgtcgcgctc ttcgcaaaga agacacccgc gcaggctgcc ggcgccgaca
     1261 acaccgcggc cacttcgacg gtcgctgttg cgaaccgctc attgtcagca cagacgaatg
     1321 tgagtgcgac gctcggttac gcgggcattt atagcgtcgt caatcaggcg ctcggcagct
     1381 tcagccgact gccgaaagtt ggtgatgtga tcgagcctgg gtccttgttg tacgcggtcg
     1441 gcgggcagcc ggtcgtgctt ctgaccggat cgactcctgc gtaccgcacg ctagtcgcgg
     1501 ggatcagcgg gcctgacgca caagaactga acgccgccct ggtcgcgctc ggctacggca
     1561 gtgcggcgac attgcaaggc agcactgtct tcaatgcggc gacggcagcc gcagttgtgc
     1621 agctacaagc gcgtctcggc gtagcgcaga ccggtgtgct tgcatcggat caggtgatct
     1681 tcttaccgtc ggctgcccgc atcaccgccg tcagcgctgc gttgggcgcc acggcacagg
     1741 aaggtgaggt tgtcgccact gcgacctcga cgacgcgtca ggtggtagtt gctctcgacg
     1801 ccacccagca agctgccgtc aaggtcggtg accaggtgac catcacgctg cccgacacca
     1861 gcacgactcc gggtgtggtg tcgaccgtgg gcactgtcgc gacagctccc gcaggcaacg
     1921 gccagggtca gtcgggcaca ccgacgatcg aggtcgacgt cactccgacc gacccgaccg
     1981 caaccggcac gttggaccag gctccagtgc ttgtggcgat cacgaccgcg agcgtcaccg
     2041 atgcattggt cgttccggtc cccgcgttgc tggcgctatc gggtggcggt tacgcggtcg
     2101 aggttgttgc ggctgatggc acgcatcaac tcgtcggcgt cacgctcgga ctgtttgacg
     2161 atgccgccgg gctcgtacag atcaacggct ccagcgtgcg cgcgggccag cacgtggtgg
     2221 tgccgtcgtc gtgactgtgg ttctcgaact cgacgacgtc accaaggtgt acccagggca
     2281 gccgccggtg accgcactcg acggtgtcag cttcagcgtc agcgcgggcg aactcgtcgg
     2341 tgtcgtcgga ccgtccggct cagggaaaac gacgttgttg caaatgatcg gcaccctcga
     2401 ccgtccgacc agcggcaccg tgcacatcac tgatatggac gtcgcggtgc tcagcgatcg
     2461 ggaattgtcg aggttgcgcg cgggcaagat cggtttcgtg ttccagcagt tcttccttgc
     2521 cgaacacgca actctcttgg acaacgtggc cgatgggctt ctctatgcag gcgttgccct
     2581 acgtgatcgg cgagcagcag cgctgatcgc tctcgagcag gtggggttgg ctgatcgcgt
     2641 caccgcccgg ccgactcagc tctcaggtgg gcaacgccag cgggtcgcga tcgcgcgcgc
     2701 attggtcggg cggccggcga tcgtgctcgc agacgagccc accggcaatc tcgacaccgc
     2761 taccggccag gccatcttgg cgctgatcga gcaactacac gcggggggca ccaccatcgt
     2821 cgtcgtcacc cacgatcgtg acatcgctgc gcgaatgcct cgtcagatcg agatgcgcga
     2881 tgggcacatc atcgccgaca ctgcattctc ggcatccggc caactgtcga caccgagtcg
     2941 atcgtgacga cagccgtgcc gccacggtcg tcgcgacggt tgcgactcgc tgacctcgca
     3001 cgcctctcca ccatcggatt gcgcacccgc aagctgcgtg cgagcttgtc agcgctcggg
     3061 gtcgcgatcg gggtggcggc cgtggtcgcc gtcctcggat tgtcggcttc gtcgcaagca
     3121 ggtctgctcg ccgagatcga caaactgggt acgaacctgc tgaccgtgac caacggccag
     3181 acgctgttcg gccaagccgc cgaactgccc gatgccgcac cgggcatgat ctctcgcatc
     3241 gggccggtca tcactgtgca agcaaccgga gcggtcaccg cctcggtgta ccgcagccct
     3301 ctcattcccg cgatccagac caacgcacta tcggtgctcg cgagcacgct cgatcttccg
     3361 gcgacggttg gcaccactct tgccaccggc cggtacctca acgcg
//
LOCUS       DASWVF010000488         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_242377, whole genome shotgun sequence.
ACCESSION   DASWVF010000488 DASWVF010000000
VERSION     DASWVF010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242377"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2366
                     /locus_tag="VGD55_08710"
     CDS             228..2366
                     /locus_tag="VGD55_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0870466.1"
                     /translation="MAEFVLAHRRWVLSFWGIVLVAGIALSGKTTNRLTLDFSLPGQP
                     GTVTAHQIKADLGNGGDTDPYLVSVTMPAGQQVSGNTAAVAKVFSAVEQRVPNVRVLD
                     EANTGDKAFRTSDGQTAYAILFYRFNPSPTAKLQTDAIRAAAAAAAPAGATIGVTGED
                     ALAVGSSKGGPGVLGETLLGALGALIVLAFVFASFLAFLPLAVAGVSILATFLMLLPI
                     TYLTNVSFIVEFLIALIGLGVAIDYSLLLVTRWREERDHGRDNHDAVVVAMETAGRAV
                     VFSGVTVAIGLLALVVLPVPFMRSMGYGGALIPLASVLSTLTLTPAILGGIGPRVDWP
                     RIRHENRASKFWGRWAALVVRRRWIAAGAALVALFALISVFFGIKIGLASSKSLAKNG
                     PAYTTLQTLEKGGVTTGTLTPMEILVSSDQAQSTAVRVAAIQGVSHVFVSTDPSSTGN
                     GRTAIVAIPDTETVNSHCVGVVKRVKSAIDGLPGVVGVAGVGAAQIDFLHAVYGNFPL
                     MLFIIAVLTYLLLVRAFRSLLLPLKAVVLNLVSLGATLGLMVLFWQDGHGSNAIFGIA
                     ATGAVTFWIPLMVFAFLFGLSMDYEVFILSRIREEYDAAKSTNAAVIEGVGRTGRLVT
                     SAALILFLAFAALASGPGTDLKTLATGLGFGILLDATIVRAVLVPSLVSLFGSWNWYL
                     PAVVAKILRVQPSHPHPERDVPRAPVAADV"
     gene            complement(2534..>2737)
                     /locus_tag="VGD55_08715"
     CDS             complement(2534..>2737)
                     /locus_tag="VGD55_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0870467.1"
                     /translation="LNIRVKDIAAVYAQWSARGAQFLTPPKQHAYEIRCYIRDPDGHL
                     IEVGQTTDPQGDWSAWLPSSTPT"
BASE COUNT          420 a          859 c          903 g          555 t
ORIGIN      
        1 aagcccgacg aggttgcgtc gttggtgtgt ttcctcgcct cagatgcagc gtcgttcatg
       61 actggcgcga ccgtcgtcac cgacggtggc tacaccgtct ggtagccgac caaccggact
      121 gagcacgaat cgagaagcgc cggcgtccca ccctgctcta gcgtgcttct gtcggccttg
      181 atcgccgcaa cggatggcag aacgcgaagg agatgctcgt gcgacggatg gctgagttcg
      241 tgctggcgca ccgccggtgg gtgctgtcct tttggggcat cgtgcttgtt gccgggattg
      301 cgttgtcggg caagacgacc aatcggttga cgctcgactt ctcattgccc gggcagccgg
      361 gaacggtgac agcgcaccag atcaaggccg atcttggtaa cggtggtgac accgacccat
      421 acctggtctc ggtgacgatg ccggcggggc agcaagtctc gggcaacacc gcggcggtgg
      481 cgaaggtctt cagcgccgtc gaacagcgcg tgccaaatgt ccgggtactc gacgaggcca
      541 acaccggcga caaggcgttt cggaccagcg acggacagac cgcgtacgcg atcttgttct
      601 accgtttcaa cccgtcgccc acggccaagt tgcagaccga cgcgatccgc gccgccgccg
      661 ccgccgccgc acccgccggt gccacgatcg gggtcaccgg tgaggacgca ctcgcggtcg
      721 gaagttccaa aggcggcccc ggagtactag gcgagacctt gctcggcgcg ctcggcgcgc
      781 tgattgtgtt ggcgttcgtt ttcgcctcgt tcctggcatt ccttccgctc gcggtggcgg
      841 gggtgtcgat tctggcgacg ttcctgatgc tgttgccgat tacctacctg accaatgtgt
      901 cgttcatcgt cgagttcctc atcgccctga tcggtctggg ggtggcgatc gactattcac
      961 tgctgctggt cacgcgctgg cgggaggagc gcgatcacgg ccgcgacaac cacgacgcgg
     1021 tcgtggtggc gatggagacc gctggcaggg cagtcgtgtt cagcggggtg acagtcgcta
     1081 ttggcttgct agcgctggtg gtgttgccag tgccgttcat gcgcagcatg ggttacggcg
     1141 gcgccctgat tcctctggcc agcgtgctct ccacattgac actgacaccg gcaatcctcg
     1201 gtgggatcgg cccacgggtc gattggccgc ggatccggca tgagaaccgg gcaagcaagt
     1261 tctggggccg ctgggcagca ctcgtcgtgc gccgtcggtg gatcgccgca ggcgctgcgt
     1321 tggtcgcgtt gttcgcgctg atcagcgtgt tcttcggcat caagatcggc ttggcgtcct
     1381 cgaagtcatt ggcgaagaac ggtcccgcat atacaacgct ccaaactctg gagaagggcg
     1441 gcgtcaccac cggcactctc actccgatgg agatcctcgt cagttccgac caggcccaga
     1501 gcaccgccgt ccgggtcgct gcgatccagg gcgtcagtca tgtctttgtt tcgactgacc
     1561 cgagcagcac tggcaacggg cggacggcga tcgtcgccat ccccgacacc gaaaccgtga
     1621 actctcattg cgtcggcgtg gtcaaacggg tcaagtccgc gatcgatggc ctgccagggg
     1681 tcgtcggtgt agccggggtc ggggcggcgc agatcgactt cttgcacgcg gtctatggca
     1741 actttccctt gatgttgttc atcatcgcgg tgctcaccta cttgctgttg gtgcgcgcgt
     1801 tcaggtcgct gctgctcccg ctgaaggccg tcgtgctgaa cctggtgtcc ttgggagcaa
     1861 cgctggggtt gatggtcctg ttctggcagg acggccacgg ctccaacgcg atctttggca
     1921 tcgctgccac cggtgcggtg acgttctgga tcccgctgat ggttttcgcg ttcctgttcg
     1981 ggctgtcgat ggactacgag gtgttcatcc tgtcccgaat acgcgaggaa tacgacgcgg
     2041 ccaagtcgac caacgccgcc gtcatcgaag gcgtcggacg gaccggtcgc ctcgtgacca
     2101 gcgccgcgct gatcctgttc ctggctttcg cggcactcgc ctcagggccc gggaccgacc
     2161 tgaaaacgct ggcgaccggc ctcggcttcg gcatcctgct cgacgccacg atcgtccgcg
     2221 ccgtcctcgt gccgtccctg gtctcgttgt tcggttcctg gaactggtac ctgcccgcgg
     2281 tcgtcgcgaa aatcctgcgc gttcagccgt cccaccccca ccccgaacgc gacgtaccac
     2341 gcgcacctgt ggcggctgac gtttaacggc gcaagggtgc tcagggtcgg ggcggccgga
     2401 tctggtgcga gcgcaactga tctggttcga cgtggactac tgacccgtcg ggtggacaga
     2461 caccagcatc ccatccgtca aactacgcaa ccttgctcac gacggcactg aggatcgggc
     2521 gcacgcctga gcgtcaggtt ggcgtgctgg acggtagcca ggctgaccag tcgccttgag
     2581 ggtcggttgt ttgcccgacc tcgatgaggt gtccgtcggg atcgcggatg tagcaccgga
     2641 tctcgtacgc gtgttgcttc ggcggtgtca ggaattgggc gcctcgggcg ctccactgcg
     2701 cgtagacggc ggcgatgtcc ttgacccgaa tgttgag
//
LOCUS       DASWVF010000489         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_140722, whole genome shotgun sequence.
ACCESSION   DASWVF010000489 DASWVF010000000
VERSION     DASWVF010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140722"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..460)
                     /locus_tag="VGD55_08720"
     CDS             complement(149..460)
                     /locus_tag="VGD55_08720"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY0870468.1"
                     /translation="MKRKTRLALTVFALLALVAGDAFAGSAGMPWEGPLNQLLQSLTG
                     PVAKAVGVFSIVGLGFGIAFSEGGSGLRKALWVVLGLAISFSATSFFLSFMGFSGGLA
                     V"
     gene            complement(572..784)
                     /locus_tag="VGD55_08725"
     CDS             complement(572..784)
                     /locus_tag="VGD55_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870469.1"
                     /translation="MTKGKNMKQLVAAIERGEGDDKKTFWTRVGVAFENRDGSWNLRF
                     DFFPARPTDTTIQLRDFSKDQEKAQD"
     gene            complement(1126..1593)
                     /locus_tag="VGD55_08730"
     CDS             complement(1126..1593)
                     /locus_tag="VGD55_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870470.1"
                     /translation="MATVMYWALRLVVGAAGLFALIVNWRVGLVLLFTVATDFIAMIW
                     MILPGFSLQQEVVIVFLGTLPLGIVVGAGKNALSPVWLTAALCSGVVAIFQLSSYLIG
                     FDEHTLDPGHAIQTLPAAVILVVASGIGLLLPAVLFRRRLQASRPGARGMPTR"
     gene            complement(1581..>2110)
                     /locus_tag="VGD55_08735"
     CDS             complement(1581..>2110)
                     /locus_tag="VGD55_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870471.1"
                     /translation="AYTEAGVTLVALLSPRLAKEIVQIPAAEFRPSSPSFGQTLKQVA
                     VTTATAIPEVIAYVLSSAAGPAHTQVWQVSSARDAAGFQADRLASIKLGQIRMLVRQG
                     QCDHLDTLDALRRSLESSTFVDVGTDNVAAIVVDEITRRMVYVNLRYVRETLRDRALR
                     DVFQEFDEKACTWRP"
BASE COUNT          412 a          684 c          668 g          346 t
ORIGIN      
        1 agagctgatg caatccaaga ccgaatgccc cggcggtcgt ccacaagagc agcgccagtc
       61 tgcgcggcaa cccgaccaac aggatcggct gcgtgagcga tcggtggaac ggaacctcca
      121 gccccggcgt ctccgattgc atgccgacct agaccgccag tccgccgctg aagcccatga
      181 agctgaggaa gaagctcgtc gcgctaaacg agatggcgag tccgagcacg acccagagcg
      241 ccttgcgaag accgctgccg ccctccgaga acgcaatccc gaagcccagg ccgacgatcg
      301 aaaagacccc gacggccttg gcgacgggcc cggtgagcga ctgcagaagc tggttcagtg
      361 gcccttccca cggcatccct gcgctgcccg cgaaggcgtc gccagcgacg agagcgagga
      421 gcgcgaatac agtgagtgcg agacgggtct tccgtttcat gtgacgtcct ttcgctgtga
      481 gaggtctgtc tcaccggttc tcgtgacccc gacgccgaac gttgcgcgag cggcgagcgg
      541 aggagaccag cccctccgct cggccccctg actagtcctg ggctttctcc tgatccttcg
      601 agaagtcccg gagctggatc gtcgtgtcgg tcggtcgcgc cgggaagaag tcgaaccgaa
      661 ggttccagct cccgtcgcgg ttctcgaatg cgacgccaac ccgggtccag aaggtcttct
      721 tgtcgtcccc ttctccacgt tcgatagccg ccacgagctg cttcatgttc tttcccttcg
      781 tcatgttcgt gttcctttcg ctgtttccgc ttccgcggtt cgttcgacct gagcggggcc
      841 cggtcgctcc tcgggatggc cgaccgcttt cgcggggggc acccgctcgc gggtggagcc
      901 gggaaagcag aggccggcca cccgcagggc acgacctgtc gcgaggcgcg acggctgcgg
      961 gtcccgcgcg aacggaccga gaagctgaaa gtcagcgagg gaacgacgac gaacgaccgg
     1021 acaacgcggg cggtcccgag aaccacacga gggttctcgc taccttacgg tccagcgttg
     1081 ctcgtgaagg atgagcgcgc cgccagttcc ggtcggcgtt ccgcttcagc gggtgggcat
     1141 tccgcgcgcg ccgggccgtg atgcttgcag tcttcgcctg aacaggaccg ccggcagcaa
     1201 caagccgatg ccggacgcga cgaccaggat gacagctgcc gggagcgtct gaatcgcgtg
     1261 tccggggtct agcgtgtgtt cgtcgaatcc gatcaggtaa gaagagagtt ggaagatcgc
     1321 caccacgccc gaacagagcg ctgctgtcaa ccagacgggc gagagcgcgt tcttgccggc
     1381 cccgacgacg atgccgagcg gcaacgtccc caggaagacg atcacgacct cttgctggag
     1441 ggaaaagccg ggcagaatca tccagatcat ggcgatgaaa tcggtagcga ccgtaaaaag
     1501 cagcaccaac ccgacgcgcc agttgactat cagagcgaac aaacccgcgg cacccacaac
     1561 caaccgcagc gcccagtaca tcacggtcgc catgtgcatg ccttctcgtc gaactcttgg
     1621 aagacgtccc gaagcgcacg gtcccgcaaa gtctcccgga catagcggag gttcacgtac
     1681 accatgcgac gcgtgatctc gtcgacgacg atcgcggcca cgttgtccgt gcctacatcg
     1741 acgaacgtcg agctctccag cgaacgtcga agcgcatcga gggtatccag gtggtcgcac
     1801 tgaccctgcc tgaccagcat gcgaatctgt ccgagcttta tcgatgcgag acggtcagcc
     1861 tgaaagccag cggcgtctcg agccgacgaa acttgccaga cctgagtgtg tgcaggaccg
     1921 gcggcgctgc tcaggacgta ggcgataacc tccgggatcg cggtggcggt cgtgaccgcg
     1981 acctgcttga gagtttggcc aaatgacgga gacgacgggc ggaactctgc tgccggtatc
     2041 tggacaattt ccttcgccag cctgggcgac agcagcgcga ccagcgtgac tccggcttcg
     2101 gtgtatgcct
//
LOCUS       DASWVF010000490         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_268169, whole genome shotgun sequence.
ACCESSION   DASWVF010000490 DASWVF010000000
VERSION     DASWVF010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268169"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1299
                     /locus_tag="VGD55_08740"
     CDS             220..1299
                     /locus_tag="VGD55_08740"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY0870472.1"
                     /translation="MGSVRVAIVGVGNCAASLVQGVEYYKNADPAERVPGLMHVQFGP
                     YHVSDIKFVAAFDVDGKKVGSDLADAIGASENNTIKFCDVPSTGIIVQRGHTYDGLGD
                     YYRDIISESEDEPVDVVKALKDAKVDVMVSYLPVGSEEADKFYAQCAIDAGVAFVNAL
                     PVFIASDPTWAKKFEDAGLPIIGDDIKSQVGATITHRVMAKLFEDRGVELLRTYQLNF
                     GGNMDFMNMLERKRLQSKKISKTQSVTSQIPHEMAKADVHIGPSDYVPWLDDRKWAYV
                     RLEGRSFGDTPLNLEYKLEVWDSPNSAGVIIDAVRAAMIAKDRGIAGPINSASSYFMK
                     SPPVQYSDDDARQAVADFIAGTVDR"
     gene            complement(1296..>1697)
                     /locus_tag="VGD55_08745"
     CDS             complement(1296..>1697)
                     /locus_tag="VGD55_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY0870473.1"
                     /translation="LHLRFHGYGTGEWTDSAVRRYVRDAGPLLERLHKLTRSDCTTRN
                     KRRAAALSSAYDELERRIEELRQQEEVDALRPELNGDEIGAVLGIAPGPEIGRAYRFL
                     LELRLDVGPIGKDAAAAALREWATEHGVGPA"
BASE COUNT          322 a          547 c          540 g          288 t
ORIGIN      
        1 aggaacgcct cgaccacgtg aagacctcgg tcgcgcgtac ccgcgagcgg gtcgacagct
       61 acaccctcga actacagcgc cacgggctcg agtccgtcga gcgcgaggtt cgttggttga
      121 ccgagttgat cgaaaccgaa cgacgcgccg acaccgcgtc gtccgctaac tagtcctttc
      181 cccctgcaac aggctgtacg agatgaagga gcaccgctca tgggttcggt tcgggtagcc
      241 atcgttggcg tcggcaactg cgccgcgtcg ctcgtccagg gggtcgagta ctacaagaac
      301 gcggatcctg cggagcgggt accgggtctc atgcacgtgc agttcgggcc ctaccacgtg
      361 tccgacatca agttcgtcgc ggcattcgac gtcgacggca agaaggtcgg gtcagacctc
      421 gccgacgcga tcggtgcgag cgagaacaac accatcaagt tctgcgacgt gccgtcgacg
      481 ggcatcatcg tgcagcgcgg ccacacctac gacggtctcg gcgactacta ccgcgacatc
      541 atctcggagt ccgaggacga gccggtcgac gtcgtcaagg cactgaagga cgcgaaggtc
      601 gacgtgatgg tctcctacct gccggtggga tccgaggaag ctgacaagtt ctacgcccag
      661 tgcgcaatcg acgccggtgt ggccttcgtc aacgcgcttc cggtgttcat cgcgagcgat
      721 ccgacgtggg ccaagaagtt cgaggacgcc gggttgccga tcatcggcga cgacatcaag
      781 agccaggtcg gcgcgaccat cacgcaccgc gtcatggcca agttgttcga agaccgcggc
      841 gtcgaattgc tgcgcacgta ccagctcaac ttcggcggca acatggactt catgaacatg
      901 ctcgagcgca agcgcctcca gtcgaagaag atctcgaaga cgcagtcggt cacgtcgcag
      961 attccgcacg agatggcgaa ggccgacgtg cacatcggac cgtccgacta cgtgccgtgg
     1021 ctcgacgacc gcaagtgggc ctacgtccgc ctcgagggtc gctcgtttgg tgacaccccg
     1081 ctgaacctgg agtacaagct cgaggtgtgg gactccccga acagcgccgg cgtcatcatc
     1141 gacgcagtcc gtgcggccat gatcgcaaag gatcgcggca tcgccggacc gatcaactcg
     1201 gcgtccagct acttcatgaa gagcccgccg gtgcaataca gcgacgacga cgcacgtcaa
     1261 gccgttgccg acttcatcgc cggaaccgtc gaccgctagg ccggcccgac gccgtgctcg
     1321 gtggcccatt cgcgtaatgc ggccgccgcg gcatccttac cgatcggacc cacgtcgagc
     1381 ctgagctcga gtaagaagcg gtaggcgcga ccgatctcgg ggccgggcgc aatgccgagt
     1441 acagcgccga tttcgtcgcc gttgagttct ggccgcagcg cgtcgacctc ttcttgctgg
     1501 cgcagttcct cgatgcgccg ttcgagttcg tcataggcac tcgacaacgc cgctgcgcgg
     1561 cgtttgttgc gcgtcgtgca gtcagaacgg gtcagcttgt gcagccgttc gagtagcggg
     1621 ccggcgtcgc gcacgtagcg gcgcactgct gagtcggtcc actcaccggt gccgtagccg
     1681 tgaaaacgca ggtgcag
//
LOCUS       DASWVF010000491         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_420938, whole genome shotgun sequence.
ACCESSION   DASWVF010000491 DASWVF010000000
VERSION     DASWVF010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420938"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGD55_08750"
     CDS             <1..343
                     /locus_tag="VGD55_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY0870474.1"
                     /translation="RLAAPASVDSIPSSAMQEDHVSMGWSAGRKLLKAVDGLTRVIAI
                     EILTAARALDMRAPLLPAAGSAAVVRTLRTRVAGPDVDRWLSPEIEAAVELTRAGTFV
                     EAAQAVVGTLL"
     gene            354..2018
                     /gene="hutU"
                     /locus_tag="VGD55_08755"
     CDS             354..2018
                     /gene="hutU"
                     /locus_tag="VGD55_08755"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0870475.1"
                     /translation="MSGPRPVRAPRGSELSCQGWPQEAALRMLMNNLDPEVAERPDDL
                     VVYGGTGKAARDWTSFDAIVRTLQSLKDDETMLVQSGRPVGVMRTHEWAPRVLIANSN
                     LVGDWATWPEFRRLEHLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKKFNGTL
                     AGTLTLTGGCGGMGGAQPLAVTMNGGAVIVVDVDPARLQRRVEQRYLDEWFDDLDVAV
                     DRALWAKRERRALSVGVVGNAAWVFPELLRRGIAIDIVTDQTSAHDPLSYLPEGIDLA
                     DWHDYAEAKPEEFTERARTSMAAQVEAMVGFMDAGAEVFDYGNSIRGEAQLAGYDRAF
                     AFPGFVPAYIRPLFCEGKGPFRWAALSGDPADIAATDRAVLDLFPENEPLARWIRSAQ
                     EKVAFQGLPARICWLGYGERHLAGLRFNELVADGRISAPIVIGRDHLDCGSVASPYRE
                     TEAMADGSDAIADWPLLNALVNTASGASWVSIHHGGGVGIGRSIHAGQVSVADGTALA
                     AQKLERVLTNDPGMGVIRHVDAGYELANEVAVQRDVRIPMHESANA"
     gene            2015..3241
                     /locus_tag="VGD55_08760"
     CDS             2015..3241
                     /locus_tag="VGD55_08760"
                     /inference="COORDINATES: protein motif:HMM:NF006770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEY0870476.1"
                     /translation="MSAATGESFDLLWSQLLPIGRTPGGYRRFAWNDADLEARAWFTS
                     AALARGLDMQTDRNGNLWAWWLPSGSLGEPRDAFVTGSHLDSVPDGGAYDGPLGVVSA
                     LCAIDVVRSRGIEPTKPVAVVVFADEEGARFGIACAGSRLAVGALPPDTARALTDIGG
                     VTLDAAMKAAGIDTGLLGRDEQALARVGVFIELHIEQGRSLVDRGAAVGIGTGIWPHG
                     RWRFSFTGQANHAGTTRLVDRHDPMLPFAAVALAARVEATRHFGVATFGRLHVEPNGT
                     NAVPSRVTAWLDARAPRDEAVEAIVHGLEVIARERSVSEGVELTVTPESISPVVDFSA
                     DALSRLRDVLGPLPELGTGAGHDAGVLSPALPSAMLFVRNPTGISHSPEEFASRADCL
                     AGIEALAAVMADWISA"
     gene            3238..>3742
                     /locus_tag="VGD55_08765"
     CDS             3238..>3742
                     /locus_tag="VGD55_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0870477.1"
                     /translation="MTQWWCEHAWLPPGQAAPNVLITVVDGLIAGVEIGVDRPPSAQL
                     LRGLVIPGFANTHSHAFHRALRGRTQAGRGDFWSWREQMYAVAQRLTPESYLALARAT
                     FAEMALAGISCVGEFHYVHHQRGGTPYDDPNIMGAALIEAAAQVGVRLTLLDACYLTG
                     GFDEALSE"
BASE COUNT          570 a         1192 c         1290 g          690 t
ORIGIN      
        1 gcggctggcg gcgcccgcga gtgtcgactc gattccgtcg tcggcgatgc aggaggatca
       61 cgtgtcgatg gggtggtcgg ctgggcgcaa gttgctgaag gcagtcgacg gcctcactcg
      121 ggtgatcgcc atcgagattc tcacggcagc gagggcgttg gacatgcgcg caccgctgct
      181 tcccgcagct ggctcagccg ccgtcgtccg cacgttgcgt actcgcgttg cagggccgga
      241 cgtcgaccgg tggctgtcac ccgaaatcga agccgccgtc gagctgaccc gtgcagggac
      301 tttcgtcgag gccgcgcaag cagttgtcgg aacacttctg taaggagcac gtcgtgagtg
      361 gacctcgccc ggtacgcgcg ccgcgcggtt cggagttgtc atgtcagggc tggccgcagg
      421 aggccgcact tcgcatgctg atgaacaatc tcgaccctga ggttgcagag cggcccgacg
      481 atctcgtcgt ctatggcggc accggcaagg cggcgcgcga ctggacctcc ttcgatgcga
      541 tcgtgcgtac gctgcaatcg ctgaaggacg acgagacgat gctggtgcag tcaggccgac
      601 ccgtcggtgt gatgcgcacg cacgagtggg cgccgcgggt gctgatcgcg aactcgaacc
      661 tggtcggcga ctgggcaacc tggccggagt ttcggcgcct cgagcatctt ggcctcacga
      721 tgtacggcca gatgacggcc ggatcgtgga tctacatcgg cacgcaaggc atcttgcagg
      781 gcacttacga gacgttcgcc gcggtcgccg cgaagaagtt caacggcacg ctcgccggaa
      841 cgctgaccct caccggcggt tgcggcggca tgggtggcgc gcaaccgctg gccgtcacga
      901 tgaatggcgg ggcggtgatc gtcgtcgacg tcgacccggc gcggttgcag cgacgtgtcg
      961 agcagcgtta tctcgacgag tggttcgacg atctcgacgt ggccgtcgat cgtgcattgt
     1021 gggccaagcg tgaaagacgg gccctttccg tcggggtcgt cggcaatgct gcttgggtgt
     1081 ttcccgagct gctgcgccgc ggcatcgcga tcgacatcgt caccgatcag acatctgcgc
     1141 acgatccgct ttcgtacctg cccgagggca tcgacctcgc cgactggcac gactacgccg
     1201 aggcgaagcc cgaggagttc accgagcgcg cccgcacatc gatggcagcg caggtcgagg
     1261 cgatggtcgg cttcatggac gccggcgccg aggtgttcga ctacggcaac tcgatccgcg
     1321 gtgaagcgca actcgccggc tacgatcgtg cgttcgcctt tcccggattc gttccggcgt
     1381 acatcaggcc gttgttctgc gaaggcaaag gcccattccg ctgggctgcg ctgtcgggcg
     1441 atcctgccga tattgccgcc acagatcgcg ccgtgctcga cctttttccc gagaacgagc
     1501 cgctcgcgcg gtggattcgt agcgcacaag agaaggtcgc gttccaaggc ctgccggcgc
     1561 gcatctgctg gcttggctac ggcgagcggc accttgccgg cttgcgcttc aacgaactcg
     1621 tcgccgatgg tcgcatcagt gcgccgatcg tcatcggacg cgatcacctc gactgcggtt
     1681 cggtcgcgtc gccctatcgc gagaccgagg cgatggctga cggatccgat gcgatcgccg
     1741 actggccgtt gctgaacgcg ctcgtcaaca ccgcgtcggg cgcgtcgtgg gtgtcgatcc
     1801 accacggcgg cggagtcggc atcgggcgct cgatccacgc cggtcaggtg agtgtcgccg
     1861 acggcactgc tctagctgcg caaaagctcg agcgggtgct gacgaacgat cccggcatgg
     1921 gcgtgatcag gcacgtcgac gccggatatg agctggccaa cgaagtagct gtgcaacgcg
     1981 acgtccggat cccgatgcac gaatccgcca acgcgtgagc gctgcaactg gggagtcttt
     2041 cgacctcttg tggtcgcagt tgctacctat tggccggacg ccgggggggt atcgccggtt
     2101 cgcgtggaat gacgccgacc tcgaggcccg cgcgtggttc acgtcggcgg cgctcgcgcg
     2161 tggcctcgac atgcagaccg accgcaacgg caacctgtgg gcgtggtggc tgccctcggg
     2221 atcgctgggg gaaccgcgtg atgccttcgt cacgggctcg cacctcgact cggtacccga
     2281 cggcggcgca tacgacgggc cgctcggtgt ggtctcggcg ttgtgtgcga tcgacgtggt
     2341 gcgaagccgc gggatcgaac ccacgaagcc ggtcgctgtc gtcgtcttcg ccgacgagga
     2401 gggcgcccgg ttcggtattg cgtgtgctgg ctcgcggctc gcagtgggcg cgttgccacc
     2461 cgacaccgcg cgcgcgttga ccgatatcgg cggcgtgaca cttgacgccg cgatgaaagc
     2521 ggccggtatt gacaccggtt tgttgggacg tgacgaacaa gccctggcac gcgtgggggt
     2581 cttcatcgaa ttgcatatcg aacagggtcg ttcgttggtg gatcggggtg cggcagtcgg
     2641 catcggtacc ggcatctggc cgcacggtcg ttggcgattc tcctttactg gtcaggcaaa
     2701 tcacgccggc accacccggc tcgtcgatcg gcatgacccc atgctgccgt tcgcagcagt
     2761 ggcactcgcc gcacgcgtag aggcgacgcg tcatttcggc gtcgcgacat tcgggcggct
     2821 tcacgtcgag ccgaacggca ccaacgcggt gccctcacgg gtgacggcat ggctcgacgc
     2881 gcgggcgccg cgcgacgagg cggttgaggc catcgtgcac gggcttgagg tcattgcgcg
     2941 cgagcgttcg gtgagcgagg gcgtcgagct taccgtcact cccgagtcga tctcgccggt
     3001 cgtcgacttt tccgccgacg ccctgtcgag gttgcgcgac gtgctcggac cattgcccga
     3061 gcttggcacc ggcgccggcc atgacgcggg agtgttgtca cccgcactgc ctagcgcgat
     3121 gttgttcgtg cgcaacccga cgggcatctc gcattcgccc gaggaattcg cttcgcgagc
     3181 cgattgcctc gccggcatcg aggcactcgc cgccgtgatg gctgactgga tctccgcgtg
     3241 acccagtggt ggtgcgagca tgcgtggctc ccgccgggcc aggctgcgcc aaacgtgctg
     3301 atcacggttg tcgacggcct cattgccggt gtcgagatcg gcgtcgaccg accgccttcc
     3361 gcgcagttgc ttcgaggcct ggtgattcct ggtttcgcga acacccactc gcacgctttt
     3421 catcgcgccc tgcgcggtcg cacccaggct gggcgcggcg acttctggag ctggcgtgag
     3481 cagatgtatg cggtcgcgca gcgattgacc cccgagtcct acctggcgct cgcgcgtgcg
     3541 acgttcgcag aaatggcact cgccggcatc tcctgtgtcg gtgagttcca ctacgtgcac
     3601 caccagcggg gtggcactcc ctacgacgat ccgaacatca tgggtgccgc gctgatcgag
     3661 gcggctgcac aggtcggcgt ccggcttact ttgcttgatg cctgttatct aacaggcgga
     3721 ttcgacgaag cgctgagcga gc
//
LOCUS       DASWVF010000492         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_191610, whole genome shotgun sequence.
ACCESSION   DASWVF010000492 DASWVF010000000
VERSION     DASWVF010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191610"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="rplB"
                     /locus_tag="VGD55_08770"
     CDS             complement(<1..538)
                     /gene="rplB"
                     /locus_tag="VGD55_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0870478.1"
                     /translation="MGIRKFKPTTSGRRGASVADFVEITRTEPEKSLVRPLHSKGGRN
                     VHGRVTVRHQGGGHKRAYRLIDFRRDDKDGVPAKVAHIEYDPNRTARIALLHYADGEK
                     RYIIAPAKLQQGDRVEAGPSSDIKPGNTLPLRNIPVGTVVHAIELRPGGGAKIARSAG
                     TSVQLVAKEGALAQLRMPS"
     gene            complement(571..867)
                     /gene="rplW"
                     /locus_tag="VGD55_08775"
     CDS             complement(571..867)
                     /gene="rplW"
                     /locus_tag="VGD55_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY0870479.1"
                     /translation="MIPDPRDVLYAPLISEKSYGLLDENKYTFLVDPDANKSQIKIAV
                     EQVFGVKVTDVNTMNRKGKRKRTRGGFGKRKDTKRAIVTLVAGDRIDIFGGPVS"
     gene            complement(864..1556)
                     /gene="rplD"
                     /locus_tag="VGD55_08780"
     CDS             complement(864..1556)
                     /gene="rplD"
                     /locus_tag="VGD55_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0870480.1"
                     /translation="MTTVTVAGTSRKIELPADVFDVQVNIPLIHQVVVAQLAAARQGT
                     HDAKTRGEVSGGGRKPYRQKGTGRARQGSTRAPQFAGGGVAHGPNPRNYEQRTPKKMK
                     AAALRAALSDRARGGRLHVLPGFGEGEGPSTKSALAALAAVVTGEQHRVLIVLERADD
                     LTWKSLRNVGGVHLLVEDQLNTYDVLVSDDVVFTEGALKTYVSRASGSPVDAETATVD
                     AADTAEDTEDTR"
     gene            complement(1553..2191)
                     /gene="rplC"
                     /locus_tag="VGD55_08785"
     CDS             complement(1553..2191)
                     /gene="rplC"
                     /locus_tag="VGD55_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0870481.1"
                     /translation="MKGVLGEKLGMTQVFDEAGRIVPVTVVQAGPCVVTQVRTPDVDG
                     YSAVQIAYGAVDPRRVNKPETGHFRKAGVPPRRYLIEVRTKDASEYTLGQEITVDVFA
                     AGQKVDVVGTSKGKGFAGVMKKHNFKGLSSSHGTQRKHRSPGSIGACATPSRVFKGMK
                     MAGRMGGLKTTAQNLTVHAVDTEANLILVKGAVPGAAGRMVFVHTAAKGGAV"
     gene            complement(2217..2525)
                     /gene="rpsJ"
                     /locus_tag="VGD55_08790"
     CDS             complement(2217..2525)
                     /gene="rpsJ"
                     /locus_tag="VGD55_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY0870482.1"
                     /translation="MAGQKIRIRLKAYDHEVIDSSARKIVETVTRTGAQVAGPVPLPT
                     EKNIYCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            complement(2636..3829)
                     /gene="tuf"
                     /locus_tag="VGD55_08795"
     CDS             complement(2636..3829)
                     /gene="tuf"
                     /locus_tag="VGD55_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0870483.1"
                     /translation="MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPELN
                     PFTPFDQIDKAPEEKARGITISIAHVEYQTDNRHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTKEHVLLARQVGVPYIVVALNKADVVDDEEILELVELEVRELL
                     SQYEFPGDDVPVVRVSALKALEGDAKWGESILELLAACDSHVPEPVREIEKPFLMPIE
                     DVFTITGRGTVVTGRIERGIVKVGETIEIVGIAEKTSSTTVTGVGMFRKLLDEGRAGD
                     NVGVLLRGTKREDVQRGQVLCKPGSITPHTQFEANVYILSKDEGGRHTPFFNNYRPQF
                     YFRTTDVTGVVDLPEGTEMIMPGDNTEMTVQLIQPIAMEEGLRFAIREGGRTVGAGRV
                     TKILK"
BASE COUNT          575 a         1221 c         1295 g          765 t
ORIGIN      
        1 ccgacggcat gcgcagctga gcgagcgcgc cttccttggc gacgagctga acactcgtgc
       61 cggctgagcg ggcgatcttc gcgcctccac cgggccgaag ctcgatggcg tggacgacgg
      121 tgccgaccgg gatgttgcgc agcgggaggg tattgccggg cttgatgtca ctgctcgggc
      181 cggcctcgac tcgatcacct tgctgaagct tcgccggcgc gatgatgtag cgcttctcac
      241 cgtcggcgta gtgcagcagc gcgatgcgcg cggtgcggtt ggggtcgtat tcgatatggg
      301 cgaccttcgc gggcacgccg tccttgtcgt cacgacggaa gtcgatgagc cggtacgcgc
      361 gcttgtggcc gccaccttgg tggcggaccg tgacccggcc gtggacgttg cggccgccct
      421 tggaatgcag cggccgcacc agcgacttct ccggctccgt ccgagtgatc tcgacgaagt
      481 cggcgacgct ggcgccacga cgacccgagg tcgtcggttt gaacttgcga atacccatcg
      541 cttctatcct tcagtttctc gggtgcccgg tcaggagacc ggacctccga agatgtcgat
      601 gcggtcgccg gccaccagcg tcacgattgc ccgcttggtg tccttgcgct tgccgaatcc
      661 accgcgggtg cgcttgcgct tgcccttgcg gttcatcgtg ttgacgtcgg tgaccttcac
      721 gccgaagacc tgctccacgg cgatcttgat ctggctcttg ttcgcgtcgg ggtcgacgag
      781 aaacgtgtac ttgttctcgt cgaggagccc gtagctcttc tccgagatca gcggcgcgta
      841 gagcacgtcg cgcgggtcgg ggatcatctg gtgtcctcgg tgtcctcagc ggtatcggcg
      901 gcgtcgacag tcgcggtctc ggcgtcgacc ggtgagccac ttgcccggct cacgtaggtc
      961 ttgagcgcgc cctcggtgaa cacgacgtcg tcggatacga ggacgtcgta ggtgttgagc
     1021 tggtcttcga ccaggaggtg cacaccgccg acgttgcgca ggctcttcca ggtcaggtcg
     1081 tcggcccgct cgagcacgat gagcacgcgg tgctgctcgc cggtgacgac ggccgcaagt
     1141 gctgcgagcg ccgacttggt cgacgggcct tcgccctcac caaagccagg cagcacgtgg
     1201 agccggccgc cgcgagcgcg gtcagacaag gcagcacgca gtgcggcggc cttcatcttc
     1261 ttgggcgtgc gctgctcgta gttgcgcggg ttcgggccgt gggcgacacc accgccggcg
     1321 aactgcggtg cacgcgtgga accctgacgg gctcggccgg tgcccttctg gcggtaaggc
     1381 ttgcgaccgc cacccgaaac ctcgccacgg gtcttcgcgt cgtgcgtgcc ctgacgagcg
     1441 gcagcgagct gcgcgacgac gacctggtgg atcagcggga tgttgacctg gacgtcgaag
     1501 acgtcggcag gcagctcgat cttgcgcgac gtgcccgcga cggtgaccgt ggtcatacgg
     1561 caccaccctt ggcggcggtg tgcacgaaga ccatgcggcc ggcggcacca ggcacggcgc
     1621 ccttgaccag gatcaggttc gcttcggtgt cgacggcgtg cacggtcagg ttctgggcag
     1681 tcgtcttcag gccacccatg cggccggcca tcttcatgcc cttgaacaca cggctcggcg
     1741 tcgcacacgc accgatggag cccggcgagc gatgcttgcg ctgggtaccg tgcgagctcg
     1801 agaggccctt gaagttgtgc ttcttcatga cgccggcgaa gcccttgccc ttgctggtgc
     1861 cgacgacatc taccttctgg ccggcagcga acacgtcgac cgtgatctct tggccaagcg
     1921 tgtactcgga ggcgtctttg gtgcgcacct cgatgaggta gcgacgcgga gggacgccgg
     1981 ccttgcggaa gtgcccggtc tcgggcttgt tcacgcgacg cgggtcgacg gcgccgtagg
     2041 cgatctggac ggcggagtag ccatcgacgt ccggcgtgcg gacctgcgtg acaacgcagg
     2101 gtccggcctg gacgacggtg accggaacga tccggccggc ctcgtcgaag acctgcgtca
     2161 tgccgagctt ctcgcccagc acgcccttga tttctttagc catgtcgtcg aaagccttac
     2221 agcttgatct cgatgtcgac gcccgcagga aggtcgagcc gcatgagcga gtcgactgtc
     2281 ttgggtgtcg gatcgagaat gtcgatgagc cgcttgtgag tgcgcatctc gaagtgctcg
     2341 cggctgtcct tgtacttgtg gggcgaacgg atcacgcagt agatgttctt ctccgtcggc
     2401 agcggcaccg ggcccgcaac ctgcgcacca gtacgcgtca ccgtctcgac gatcttgcgc
     2461 gccgaagagt cgatgacctc gtggtcgtag gccttgagcc tgatgcggat cttctgtccc
     2521 gccatggtga tcctgtctct ctagttcgtg cctagtactg ccgctgcttg accggagcgt
     2581 tttgggcttt caatgacccg gcggccgggg ttggcacccc ggccgccgga ccttgctact
     2641 tgaggatctt ggtgacgcga cctgcaccaa cggtgcggcc accctcgcga atggcgaacc
     2701 gcagaccctc ttccatggcg atcggctgga tcagctgaac cgtcatttcg gtgttgtcgc
     2761 ccggcatgat catctcggtg ccctcaggca ggtcgacgac gccggtgacg tccgtggtgc
     2821 ggaagtagaa ctgcgggcgg tagttgttga agaacggcgt gtgccggccg ccttcgtcct
     2881 tcgacaagat gtagacgttc gcctcgaact gggtgtgcgg agtgattgag ccgggcttgc
     2941 acagaacctg gccgcgttgc acgtcctcgc gcttggtgcc acgaagcagc acaccgacgt
     3001 tgtcgcccgc acggccttcg tcgagcagct tgcggaacat cccgacgccg gtgactgtcg
     3061 tgctcgaggt cttctcggcg atgccgacga tctcgatggt ctcacccacc ttgacgatgc
     3121 cccgctcgat acgacccgtg acgaccgtgc cgcgaccggt gatcgtgaag acgtcttcga
     3181 tcggcatcag gaacggcttc tcgatctcac ggaccggctc gggcacgtgg ctgtcgcacg
     3241 ccgcgagcag ctccaagatc gactcgcccc acttggcgtc gccctcgagc gccttgagcg
     3301 ccgagacgcg aaccacggga acatcgtcgc ccgggaattc atactggctc agcagctcgc
     3361 ggacttcgag ctccacgagc tcgaggatct cttcgtcgtc gacgacgtcg gccttgttca
     3421 gggcaacgac gatgtacggg acgccgacct ggcgggcgag gagcacgtgc tccttcgtct
     3481 gcggcatcgg accgtcagct gcggagacca ccaggatcgc gccgtccatc tgcgctgcac
     3541 cggtgatcat gttcttgatg tagtcggcat ggccggggca gtcgacgtgg gcgtagtgcc
     3601 ggttgtcggt ctggtactcg acatgcgcga tcgagatcgt gataccgcgg gccttctcct
     3661 ccggcgcctt gtcgatctga tcgaacggcg tgaacgggtt cagctcgggg tgtgcgtcgt
     3721 gaaggacctt ggtgatggcc gctgtcagcg tcgtcttgcc atggtcgatg tgaccgatgg
     3781 tgccgatgtt gacgtgcggc ttggtccgct cgaacttcgc cttcgccact ggatgctcct
     3841 tgaggacgga ttgctt
//
LOCUS       DASWVF010000493         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_242604, whole genome shotgun sequence.
ACCESSION   DASWVF010000493 DASWVF010000000
VERSION     DASWVF010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242604"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VGD55_08800"
     CDS             <1..774
                     /locus_tag="VGD55_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870484.1"
                     /translation="AAPVSSAPEVSAVASASNALPTSTPSPLELSALGPVILVPGYGG
                     SDTMLETLANRLHSAGRTTILLALPDDATGDLNGQAAILAARVTQALRAGAPSVDLVG
                     YSAGGIVIGLFVAAHPKEARQIVTIGSPLHGTQIAGLAVTLNLPSSDCPVACREMAPG
                     SSVLASLDAAEPAHTGVPWTSMWTNDDQVVLPPDSARFTGATNIVLQQVCADDTANHL
                     NLPADQLVAGLTLRALDTSDVASPSASDCSTLRALGTGT"
     gene            complement(781..>1720)
                     /locus_tag="VGD55_08805"
     CDS             complement(781..>1720)
                     /locus_tag="VGD55_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY0870485.1"
                     /translation="AGRSPIASVPSYIEPQAPATLPGARIASRHPIPNSRSFYPVGSR
                     LGVFGNIRAGFRLLPTCWGALMADPTLLFVPLVVLFVGGVASAGYIAAFGGLAHLLAG
                     GRAVVAVKTLPLTVVLAVVSVIGRAVVVADATQRLQGAKPRIDVAWRLTVSRLPALIG
                     FGIAQALESTMTAALRNSAAGRIAANVADKAWDFATFLAVPTILFENASPLVAVRRSG
                     SLVASRWGTQLTAQAVLSFAIAACTIPLAVVAIVVAVANAAFGIALLVAILLAAIAVS
                     SALNGILSAAMYRFATTGMLAPGFSEADMWSVFAGR"
BASE COUNT          324 a          575 c          559 g          262 t
ORIGIN      
        1 gccgccccgg tgagtagcgc tcccgaggtc agcgctgtcg cgtcagccag caacgcgttg
       61 cccaccagca cgccttcccc tcttgagctg tccgctttgg gtccggtcat tttggttcct
      121 ggctacggcg gcagcgacac gatgctcgag acgctcgcta atcgattgca cagtgcgggg
      181 cggacgacga tcctgctcgc gttgcctgac gatgcgaccg gcgatctgaa tgggcaagcc
      241 gccatcctcg cggcgcgagt gacgcaggcg ttgcgggccg gcgcgccatc ggtcgacctc
      301 gtcggctact cggccggcgg catcgtgatc ggcctgttcg tggcagcgca tccgaaggag
      361 gcgcgccaga tcgtcaccat cggatcgccg ctgcacggca cacagatcgc cggactcgcg
      421 gtcactctga atctgccgtc gtcggactgc ccagtcgcgt gtcgcgagat ggcgccgggc
      481 tcgtcggtgc tcgcgtcctt ggacgcagcc gagcccgcac acacgggcgt gccgtggacg
      541 tcgatgtgga ccaacgacga tcaagtcgtt ctgccgcccg actcggcgcg cttcaccggc
      601 gcaacgaaca tcgtgcttca gcaagtgtgt gccgacgaca ccgcgaacca cctgaacctg
      661 ccggccgacc aactcgtggc gggcctcaca ctgcgtgcgc tcgacacatc tgatgtggcc
      721 agcccatcgg cgtcggactg ttcgactttg cgcgcactag gcaccggcac ctgacgctcg
      781 ctagcggccg gcgaacaccg accacatgtc tgcttcgcta aatcctggcg ccagcatgcc
      841 ggtcgtcgcg aaccggtaca tcgccgccga caagatgccg tttagagccg aacttacagc
      901 gatcgctgct agcaaaatcg ctaccagcaa agcgattccg aacgcagcgt tcgcgaccgc
      961 gacgacaatc gcgacgacgg cgagcgggat cgtgcaggct gcaatcgcga acgacaacac
     1021 tgcttgcgca gtcagctgag taccccaccg cgaggcgacc agcgagcccg atctgcgcac
     1081 cgccaccaaa gggctggcgt tttcgaacaa gatcgtcgga acggcgagga aggtcgcgaa
     1141 atcccaggcc ttgtcggcga cgttcgcagc aatacggcca gcggcactgt tgcgtagcgc
     1201 ggccgtcatc gtgctctcga gtgcttgtgc gataccgaaa ccgatcagtg caggcaaccg
     1261 gctgacggtc aacctccatg cgacgtcgat gcgcggcttg gcaccttgca atcgctgcgt
     1321 ggcatcggcg acgacaacgg cgcggccaat gacagacaca acggcgagaa cgacggtcag
     1381 cggcagcgtc ttgacggcga cgacggcgcg accacccgcg agcagatgcg cgaggcctcc
     1441 gaacgcggcg atgtagccag cagacgcgac gcctccgacg aacagaacca cgagcggcac
     1501 gaacaagagg gtggggtcgg ccatcaaagc accccagcag gtgggcagca gtcggaagcc
     1561 tgcgcggata ttgccgaaca cgccgaggcg actgcccacg ggatagaaac ttcgggagtt
     1621 cggtatcggg tgtctgctgg cgatgcgcgc gcccggaagc gtcgccggcg cttggggctc
     1681 gatgtacgaa gggacagacg cgatgggact gcgcccggcc
//
LOCUS       DASWVF010000494         6489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_165971, whole genome shotgun sequence.
ACCESSION   DASWVF010000494 DASWVF010000000
VERSION     DASWVF010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165971"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGD55_08810"
     CDS             <1..240
                     /locus_tag="VGD55_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870486.1"
                     /translation="ILFVVAFFVHVPDGVKWAGFILLDVVVQVALGLAAHGAAALGWF
                     HGPNALLLFSLALYTARRSTTVVAATATASASAIS"
     gene            complement(226..1095)
                     /locus_tag="VGD55_08815"
     CDS             complement(226..1095)
                     /locus_tag="VGD55_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870487.1"
                     /translation="MPGIDDIADALYGLPPEQFTAARTQYEKEAKAAGDRDAAAAIHA
                     MAKPSVTAWLANQLAREHRDELRPLLELGAGLRDATRDLAGDQLRTLSRQQHQLVYAL
                     VQQARQLARAVGRSVSDDAARGLEETLHAALADERAASLLLAGRLTEAMHSSGLDIDA
                     PTNVIPLRRKDSGESSLGRRQPREEERRRAEQDLVEAQRALANATAELDNARALAADA
                     DRAAQAAKERIDELRQQLEEASSSASDIDRRRDEHTRALHRAELAVHNAERQAVAAQD
                     RRDRIAGDTSGDG"
     gene            1185..2057
                     /locus_tag="VGD55_08820"
     CDS             1185..2057
                     /locus_tag="VGD55_08820"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0870488.1"
                     /translation="MTETAGAARPADAALRRPGRILPLLPARMRRQVRPKLWQEIAFI
                     LISYFVYSQIRDAVPIHERPALLRGRQILYAEAHVGLNFELSVNKFFDHVHWGGWHYL
                     ANIANYYYALMHFVIVVSVLVWVYVKRPLNYRSARTVLYATNAIAAFGFWFYPLAPPR
                     LLPNHGFIDTVVKYHTWGSWGSNGISKVSNQYAAMPSLHIGWSLWAGITLFMLSGRWW
                     IRALGLLYPVVTFFVIIGTANHYTLDAAGGVVALLGGFLIQRLLTGRHVYAAGAEDIP
                     EHQHRHPAAPAPNT"
     gene            complement(2065..3360)
                     /locus_tag="VGD55_08825"
     CDS             complement(2065..3360)
                     /locus_tag="VGD55_08825"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870489.1"
                     /translation="MTELRAEQGGQSVQRPPDSAMANREFRGIVAAQFTSECGDQIAA
                     IALSYLVYTRSDSAFLAAATYAVTFAPWVLGSVLLSPLVDRLPRRRVLVVCDLARVVV
                     IGLLSFLVSFHSVPIPVLLLLVLISSCFSPPFGAARSSMLPDIFEAGPGYVSAVAIGR
                     ILQQVDSVFGFALGGVIVAAVSPRGALAIDSGTFLLSYIFTIVAVRDRPAAIPGGRPS
                     VRSLLSEVGPNLREVLSSRTRRAMLLLSAAALLFLIAPESLAVAYARQHGHGAVAAGL
                     LTASQPLGIALGAWLFIRYVPPRSQGRLLLPLASTGAAVLALTSLVPPVWLACVLWAL
                     AGVTQCFLVTTIAAYNVVTDRALRGRANGIAAATISISQGLGFLIWGAVGNWRGAAAG
                     VAWAGVAGLLIMALVRYSWPDAVIEAAWDKLDAAQRRSV"
     gene            complement(3357..6359)
                     /locus_tag="VGD55_08830"
     CDS             complement(3357..6359)
                     /locus_tag="VGD55_08830"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0870490.1"
                     /translation="MTTSEQERPTSRLARFARLGFTDAARADAIAARAGIGFGLLTEI
                     AGSADPDLALESLVHLVDATANSSAADDRHNALALDLALRADVDFRRRLFAVLGSSAA
                     LGDHLATHPADWVDLADPGFDDSRPTWLGLRTRLTAAVVGKTGPAAEYALRSTYRRLL
                     MRLAARDLTGQATVDDVAAELADLASATLDAALVVATAELGADAAACRLAVIGMGKCG
                     GRELNYISDVDVVFVAEAADDADAVAALRTAGQLAAGIIRVCASIWEVDTALRPEGKM
                     GPLVRTLASHVAYYHRWAKTWEFQALLKARPVAGDFALGQAYVDALRPMVWTAAGREH
                     FVEDVQAMRRRVEASLPAADAEREIKLGPGGLRDVEFAVQLLQLVHGRADDTLRSATT
                     LNALADLAAGGYVGRRDAVLLADAYRFLRTLEHRLQLHHLRRTHLLPIADADIRRLGR
                     GMGFRRDPVGELHAEHTRHAREVRRLHEKLFYRPLLAAVARLPGDEARLSLDAAAARL
                     EALGYTDPESALRHLETLTSGLSRRAAIQRTLLPVMLSWFADAPDPDGGLLAFRQVSD
                     SLGDTHWYLRLLRDEGAAAQHLALVLASGRYAAELFLRAPESVAMMADSGEGSESPHQ
                     LRPRPAADLRAELLSAVRRYDDAEAAVSTARGLRRRELLRVACADLLGLLDTAEVGQA
                     LSDVTSATLDAVLDAVIRKVEADRGGGPLPMKLAIIGMGRLGGGEQGYGSDADVLFVH
                     EPVSGADEAQAAAVAHLVANEVRRLLAVPAPEPPVDVDADLRPEGRQGPLTRSLGSYV
                     AYYARWSKVWESQALLRAAPVAGDADLGARFIAAIDPIRWPPEGLAPADVREIRRIKA
                     RVEKERLPRGADPTLNTKLGRGGLSDVEWTVQLLQLRFAGGVTGVAGLRSTSTLQTLE
                     AARVADLIDAGDAETLASAWRLATRVRNALVLARGKPADSIPTDFREVVRIARLVGYS
                     PQQSGDFLELYRRVTRRSRAVVDKIFYA"
BASE COUNT         1095 a         2273 c         2134 g          987 t
ORIGIN      
        1 atcctgttcg tcgtcgcgtt cttcgtgcac gtaccggacg gcgtgaagtg ggccggattc
       61 atcctgctcg acgtcgtcgt gcaggtcgcg ctgggactgg ccgcgcacgg agccgccgct
      121 ctgggttggt tccacggacc caacgcgctg ctgttgttct cgttggccct ctacacagcg
      181 cgacgatcca ccaccgtggt ggccgccacc gccaccgcaa gcgcgtcagc catctcctga
      241 cgtatcgccg gcgatacggt cgcgacggtc ctgcgcggca acggcttggc gttcggcgtt
      301 gtggacggcg agttcggcgc ggtgtagggc gcgcgtgtgc tcgtcgcgac ggcggtctat
      361 gtcggacgcc gaactgctcg cctcctcgag ttgctgacgc agttcgtcga tccgctcctt
      421 ggcggcttgc gcggcccggt ccgcgtcggc ggccagggcc cgcgcgttgt cgagctccgc
      481 ggtcgcgttg gcgagcgcac gctgggcttc gacgagatct tgctcggccc ggcggcgctc
      541 ttcctcgcgc ggctgccggc gtccgagtga actctcgcct gagtccttgc ggcggagcgg
      601 aatgacgttg gtcggggcat cgatgtcgag tccggagctg tgcatcgcct cggtcaggcg
      661 gccggcgaga agcaagctcg ctgcgcgctc gtcggcgaga gcggcgtgca acgtctcttc
      721 caacccgcga gcagcgtcat cgctgaccga gcggccgacg gcgcgcgcga gttgccgggc
      781 ctgctggacc agcgcgtaca cgagttggtg ttgctgtcgc gacaaggtgc gcagctgatc
      841 cccggccagg tcgcgggtgg cgtcgcgtag tccggcaccc agttcgagca gcggccgcaa
      901 ctcgtcgcga tgctcgcgag ccaactggtt cgccagccac gccgtcacgc tcggcttcgc
      961 catcgcgtgg atcgccgcag ctgcgtcgcg atcgcccgcc gccttggctt ccttctcgta
     1021 ctgcgtgcgg gcggccgtga actgttccgg cggcagcccg tacagcgcgt cggcgatgtc
     1081 atcgatgccc ggcatgttgg gcaccctagt gcgacgtgga cagcggcggc accacagctg
     1141 cgagtacgtg tcgccgaggt ctgacaagat gacaggtcgt gggcatgacc gagacggcgg
     1201 gcgctgcgcg tcccgccgac gcagcgcttc ggcgtccggg ccgcatcttg cccttgctgc
     1261 ctgcccgcat gcgccgtcag gtgcgaccca agctgtggca agagatcgcg ttcatcctga
     1321 tcagctattt cgtctactcg cagattcgcg acgcggtgcc gatccatgaa cgacccgcac
     1381 tcctgcgcgg ccggcagatt ctctatgccg aagcgcacgt cggtctgaac ttcgaactca
     1441 gtgtcaacaa gttcttcgac cacgtgcatt ggggtggctg gcactacctc gcgaacatcg
     1501 cgaactacta ctacgccctg atgcatttcg tgatcgtcgt ctccgtgctg gtgtgggtct
     1561 acgtgaagcg gccgctgaac taccggtcgg cacgcaccgt gctctacgcg acgaacgcaa
     1621 tcgccgcgtt cgggttctgg ttctatccac tcgccccgcc gcggctgctc cccaaccatg
     1681 gcttcatcga cacggtcgtg aagtaccaca cgtggggatc gtggggctcc aacggcatct
     1741 cgaaagtctc gaaccagtac gccgccatgc cgtcgctgca catcggctgg tcgctgtggg
     1801 ccggcatcac cttgttcatg ctgagcggcc gctggtggat tcgagcgctc ggtctgctgt
     1861 acccggtcgt cacgttcttc gtgataatcg ggacggcgaa ccactacacc ctcgatgcgg
     1921 cgggcggtgt ggttgccctt ctcggcggct tcctgatcca gcggctactg acgggccggc
     1981 acgtctacgc agcaggcgcc gaagacatcc ccgagcacca gcaccgccac ccggcagcgc
     2041 ccgcgccgaa tacctgaacc gcgactagac cgacctgcgc tgcgcggcgt cgagcttgtc
     2101 ccacgcagcc tcgatcacgg cgtccggcca gctgtacctg accagcgcca tgatcagcaa
     2161 cccggcaacg cctgcccacg caacgcctgc agccgcgcct cgccagttgc cgacggcacc
     2221 ccagatcaag aatccgagcc cttgactgat cgagatcgtc gcagcggcga tgccgttggc
     2281 tcgcccgcgc aaggctctat cggtgaccac gttgtaggcc gcgatcgtgg tgacgaggaa
     2341 gcactgcgtc accccagcca gtgcccacag cacgcacgcc aaccacaccg gcggcacgag
     2401 ggatgtcagc gcgagaacgg ccgcacctgt actcgccaac ggcagcagta atcgcccctg
     2461 gctgcgcggc ggcacgtatc tgatgaacaa ccaggcgccg agcgcgatcc ctagcggttg
     2521 cgacgccgtc aacaaaccag cggcaaccgc gccatggccg tgctggcgtg cgtaggcgac
     2581 ggcaagactc tctggagcga tcaagaacaa cagggctgcg gccgacagca ggagcatcgc
     2641 ccgacgggtc cggctgctca gcacctctcg caagttcgga ccgacttccg agagcagcga
     2701 gcgcacgctc ggccggccgc ccggtatcgc cgcaggtcgg tcacgtaccg cgacgatcgt
     2761 gaagatgtac gacagcagga acgtgcctga gtcgatcgcg agtgcccctc gaggcgacac
     2821 tgcggcgaca atcacgccgc ccaacgcgaa gccgaacacc gagtcgacct gctgaaggat
     2881 ccggccgatc gcaaccgcac tcacgtagcc cgggcccgct tcgaagatgt cgggaagcat
     2941 cgaactgcga gcagcgccaa acggcggcga aaagcagctg gagataagca cgagcagtag
     3001 cagcaccggg atcggaacgc tgtggaacga gaccaggaag ctgagcaggc cgatgacgac
     3061 gacccgcgcg aggtcgcaca ccaccaagac gcgtcgcctg ggtagccggt cgacgagcgg
     3121 tgacagcaac acgctgccga gcacccacgg cgcgaacgtc acggcgtagg tcgcggccgc
     3181 aaggaacgcg ctgtcggacc gcgtgtacac caggtacgac aacgcgatcg cggcgatctg
     3241 gtcaccgcat tcgctcgtga actgcgcggc tacgatcccg cgaaactcgc ggttggccat
     3301 ggcgctgtcg ggcgggcgct gcactgactg ccctccttgc tccgcccgta gctcagtcat
     3361 gcatagaaga tcttgtcgac gacggccctg gatcgcctcg tcacccgacg gtacaactcc
     3421 aggaagtcac cggactgctg cggagagtag ccgaccagac gagcgatccg aacaacctcc
     3481 cggaagtcgg tcggtatcga gtcggccggc ttgccgcgcg cgagcacaag agcattgcgc
     3541 accctcgtcg ccaaccgcca ggcagacgcg agcgtttcgg cgtcccccgc atcgatcaaa
     3601 tccgcgacgc gtgctgcctc gagggtttgc aaggtcgagg tcgaacgaag gccggcgaca
     3661 ccagtgacac cacccgcgaa ccgcagctga agcaactgca cggtccactc gacgtccgac
     3721 aatcccccgc gaccgagctt cgtgttcaac gtcgggtcgg ccccacgcgg cagccgctcc
     3781 ttctcgacgc gagccttgat gcggcggatc tcgcggacgt cggccggagc caaaccctcg
     3841 ggtggccacc ggatcgggtc aatcgcggcg ataaagcgcg cgcccaaatc cgcatcgccc
     3901 gccaccggag ccgcccgcaa caacgcctgg ctctcccaca ccttcgacca ccgcgcgtaa
     3961 taggcgacat acgagccaag actgcgtgtg agcgggccct gtcggccctc aggacgaaga
     4021 tcggcatcga cgtcgacggg tggttctggg gccgggacgg cgaggagccg gcgaacctcg
     4081 ttcgcgacca agtgggctac cgcagcggcc tgcgcctcgt cagctcccga caccggttcg
     4141 tgcacgaaca gcacgtcggc gtcactgccg tacccctgct cgccaccccc gagacgtccc
     4201 atgccgatga tggccaactt catcggcaac ggcccgcccc cgcgatccgc ctcgaccttg
     4261 cggatcaccg cgtcgagcac cgcatcgagc gtggccgacg tgacgtccga cagcgcctga
     4321 cctacctcgg ccgtgtcgag cagaccgagc agatcagcgc acgccaccct caacagttcg
     4381 cggcgacgca gaccgcgtgc cgttgagacc gcagcctccg catcgtcgta gcgccgcacc
     4441 gccgacagca gttcagcccg caagtcggcg gcgggtcgcg gccgaagctg atgcggactc
     4501 tcactgccct cacccgagtc ggccatcatc gccaccgact caggcgctcg caggaacagt
     4561 tcggccgcat accgaccact cgcaagaacg agagcgagat gctgcgccgc cgcgccctca
     4621 tcgcgcagca gccgcaagta ccagtgcgtg tcacccaggg aatcggacac ttgccgaaat
     4681 gcgagcaacc cgccgtccgg gtcaggagcg tctgcgaacc acgagagcat gaccggcagc
     4741 aacgtccgct ggatcgcggc gcgcctgctg agtccactgg tcagcgtttc gaggtgacgc
     4801 aacgcgctct cgggatcggt gtaacccagc gcctcgaggc gcgcggcggc cgcgtcgagt
     4861 gacagccgag cttcgtcccc gggcagccgt gcgacggcgg cgagcaacgg ccggtagaac
     4921 agcttctcgt gcaagcgccg gacctcacgt gcgtgccgtg tgtgctcggc gtgcagctca
     4981 cccaccggat cgcgccgaaa gcccatgccg cgaccgagcc tgcggatgtc ggcgtccgcg
     5041 atcggaagca ggtgggtgcg acgcaggtgg tgcagctgaa gccggtgttc cagcgtccgc
     5101 aagaagcgat aggcatcggc gagcaacacc gcatcgcgac ggccgacgta gccgccggca
     5161 gccaggtcgg cgagcgcgtt cagggtggtg gcgctgcgca gcgtgtcgtc agcccgaccg
     5221 tgcacgagtt gcagcaactg caccgcgaac tcgacgtcgc gcaggccgcc ggggccgagc
     5281 ttgatctcgc gctcggcgtc ggccgccggc aagctcgcct cgacccgccg tcgcatcgcc
     5341 tggacgtcct cgacgaagtg ctcacgaccg gcagcggtcc acaccattgg ccgcagcgcg
     5401 tcgacgtagg cctgcccgag tgcgaagtca ccggccaccg gccgcgcctt cagcagcgcc
     5461 tggaattccc aggtcttcgc ccatcggtgg tagtacgcga cgtggctggc caacgtccgc
     5521 acgagcgggc ccatcttgcc ttcaggacgc agcgcggtat cgacctccca gatcgacgca
     5581 cacacgcgga tgatgcccgc ggcgagctga cccgcggttc gtaaggcagc aactgcatct
     5641 gcgtcgtcag cggcttccgc cacgaacacg acatcgacgt cgctgatgta gttcagctcg
     5701 cgcccgccgc acttgcccat gccgatcacg gcgaggcggc acgccgcggc atcagcacct
     5761 agctccgccg tggcaacgac cagtgcggcg tcgagggtcg ccgaggccag gtcggcgagc
     5821 tccgcagcga cgtcgtcgac ggttgcctgg ccggtgagat cgcgggcggc cagccgcatg
     5881 agcaagcgcc ggtacgtcga gcgcagcgcg tactcagcag caggcccggt cttgccgacg
     5941 actgctgcgg tcaacctggt gcgcaggcca agccaggtcg gtctgctgtc gtcgaagcct
     6001 gggtcggcca ggtcgaccca gtcggcagga tgggtcgcca ggtggtcacc gagcgcggcg
     6061 ctcgacccga gcaccgcgaa caaccggcgc cggaagtcga cgtcggcccg caacgccagg
     6121 tcgagcgcga gcgcgttgtg gcgatcgtcg gccgccgagc tgttggcggt cgcgtcgacg
     6181 agatgcacga gcgattcgag cgccaggtct ggatccgcac tgcccgcgat ctctgtcagg
     6241 agcccgaagc cgatgcccgc ccgcgcagcg atcgcgtcgg cccgtgcggc atcggtgaag
     6301 ccgaggcggg cgaagcgggc gagtcgactg gtcggccgct cctgctcgct ggtggtcacg
     6361 ccccgacggt agcgggcagc ggggccgcgt cagaggatgg gcaggtagcg gtcgcgctca
     6421 tatgcggtga cttgacgctg gtagtcggcc cactcgtcgc gcttgttgcg caggaagaaa
     6481 tcgaagacg
//
LOCUS       DASWVF010000495         5843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_38817, whole genome shotgun sequence.
ACCESSION   DASWVF010000495 DASWVF010000000
VERSION     DASWVF010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38817"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGD55_08835"
     CDS             <1..585
                     /locus_tag="VGD55_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY0870491.1"
                     /translation="KIFSLSGHVARPGQYEAPLGITLRELLDATGGMREGHELKFWTP
                     GGSSTPLFTAEHLDVPLDFEGPAAHGSMLGTTALQLFDETTCVVRAVLRWSEFYAHES
                     CGKCTPCREGVFWYVQILERLERGEGDEHDLDTLLDLSENILGRAFCALGDGATSPVT
                     SSIQYFRDEYIAHFDGRGCPFDPVASTVFAGASA"
     gene            585..3077
                     /locus_tag="VGD55_08840"
     CDS             585..3077
                     /locus_tag="VGD55_08840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEY0870492.1"
                     /translation="MTVTTNPPAAGGGATPAKSNLVTLTVDGFEISVPKGTLVIRAAE
                     HLGIQVPRFCEHPLLDPVGACRQCLVEVEGQKKPQASCTITVTEGMVVKTQYTSAVAE
                     KAQNGVIELLLVNHPLDCPVCDKGGECPLQNQTMTNGYSESRFRDVKRTYPKPIAISA
                     QVLLDRERCVLCTRCTRFAQQIAGDPFIEMLERSALEQVAVYEDQPFNSYFSGNTVQI
                     CPVGALTGTQYRFRARPFDLVSSPSVCEHCASGCGQRNDHRRGKMLRRLAGVDPQVNE
                     EWNCDKGRWAFSYATAPDRLDSPLVRDADGDLVPASWPEALRAAAAGLAAARNGAGAG
                     VLVGGRLTVEDAYAYAKFARVALGTNDVDFRGRVHSDEELAFLGSRVAGRFLEVTYDD
                     LEAASTVLLVGFEPEEESPIVFLRLRKAAMKKHAKVVSIAPFATRGLEKMRGRLIATV
                     PGAEAVVLQALAAGETVEVPPGVDSHGYSELLTEAAAGLALPASLILVGERLAAVPGG
                     LSAAVALADATGARLAWIPRRAGERGAVEAGALPNLLPGGRALADNDARGEVALNWAT
                     SISAKPGRDTSGMLAAATDGSLGALVVAGVDPDDLPDPAAAHAALAAAGFVVSLELRR
                     SAVTDVADVVFPVAAAVEKAGTFIDWEGRSRPFEASLTDSGQLSDLEVLHALADALDV
                     HLGLPDLRTARAELQALSAHTGSMAAPSAPTAMPAPPVKLTPGHAVLATWHELLDEGS
                     LQDGTPYLAATAKPIHARLSPATARAVGVVDGAPVTIRAGKAGGAITLDLLITAMPDG
                     VVWVPTRSVGSAVHRTLGVGAGAVVSLEGGSQ"
     gene            3074..4477
                     /gene="nuoH"
                     /locus_tag="VGD55_08845"
     CDS             3074..4477
                     /gene="nuoH"
                     /locus_tag="VGD55_08845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0870493.1"
                     /translation="MNAHVLGAQLVASAVSEPNHRDLWWLIILKTVVVFGFLMVMTLF
                     MIWAERRVVARMQQRLGPNRVGPFGLLQGLADGIKLALKEDLIPTAADRIVFILAPII
                     SAIPAFMAFAVIPFGPTVSIFGSHTNLQLTDLPVAVLFVLAMSSMGIYGIVLAGWSSG
                     STYPLLGGVRSAAQMISYEVAMGLSFVGVFIQSGSLSTSEIVAAQQQRWFVLSAFVSF
                     LVYAISMVGETNRAPFDLPEAEGELVGGFHTEYTSLKFALFFLAEYINMVTVSALMTT
                     LFLGGWRAPWPISLWHDANTGWWPMLWFFAKLFCVLFVFIWLRGTLPRLRYDQFMKFG
                     WKVLIPASLIWIMVEAAIKVLDRKQLIWGIGVTAILVIAASFVYDSVEQNKQRAVEEE
                     EEAASRAPFDPYAGGFPVPPMPGQQLPLRRPVPILAGNAGAASAPTDDDDDPSDPNDP
                     NDTDPSDPSGSGEVIGG"
     gene            4470..5015
                     /gene="nuoI"
                     /locus_tag="VGD55_08850"
     CDS             4470..5015
                     /gene="nuoI"
                     /locus_tag="VGD55_08850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY0870494.1"
                     /translation="MASFFGPVKGFGVTFATMFKAVNTELYPEHKKITMPRFHGRHVL
                     NRHPDGLEKCVGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQINYLRCILC
                     GLCIEACPTRALTMSNEYEMADDNRADLIFTKEQLLAPLAPGMELPPHPMRLADAKDY
                     YRGTPAAHVMFGDDDAPEVTE"
     gene            5015..5833
                     /locus_tag="VGD55_08855"
     CDS             5015..5833
                     /locus_tag="VGD55_08855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0870495.1"
                     /translation="MPAVVLASSMHGGGPETAIFWIFAPLCVLAALGMVFSSNTVHGA
                     LLLGGVMLSLAVFYAAQDAPFLAFVQVIVYTGAVMMLFLFVLMLIGVDTRESLVETLR
                     GQRLAASSAALGFLILLFAGIRQASITTSAGLASANNPTDAQGNAVAGQGNIFEIARL
                     LFTRYIFAFEVTSALLITAALGAMVRAHREPHEPKRGQREQMRDRFKTGTHPTPLPGP
                     GIYARHNAVDTPALLPDGSPSELSVPPAVEELRLERGRNPQPAQLGGKPGTRQP"
BASE COUNT          921 a         1931 c         1914 g         1077 t
ORIGIN      
        1 aagatcttct cgctctcggg tcacgtcgcc cggcccggtc aatacgaagc gccgctcggc
       61 atcacgttgc gtgagttgct cgatgccacg ggcggcatgc gtgagggcca cgagctcaag
      121 ttctggacgc cggggggcag ctctactccc ttgtttaccg ccgaacacct cgacgtcccg
      181 ctggatttcg agggacccgc cgctcacggc tcgatgctcg gcacgaccgc cctccaactg
      241 tttgacgaga ccacgtgtgt cgtgcgcgcc gtgctgcggt ggagcgagtt ctatgcgcac
      301 gagtcgtgcg gcaagtgcac gccgtgtcgc gagggcgtgt tctggtacgt ccagatcctc
      361 gagcgtctcg agcgcggcga aggcgacgag cacgacctcg acacgctgct cgatctctcc
      421 gagaacattc tcggccgtgc attctgcgca ctcggcgacg gtgcgacgag cccggtgacg
      481 tcgtcgattc agtacttccg cgacgagtac atcgcccact tcgacggtcg cggctgcccg
      541 ttcgatccgg tggcatcgac ggtgttcgcg ggggcgagcg cctgatgacc gtcacgacga
      601 atccgccagc ggcgggcggc ggggcaacgc ccgcgaagtc caacctcgtg accttgacgg
      661 ttgacggctt cgagatctcg gtacctaagg gcaccttggt catccgggct gctgagcacc
      721 tcggcatcca ggtgccgcga ttctgcgagc acccgctgct cgatcccgtc ggtgcgtgcc
      781 ggcagtgcct tgtcgaagtc gagggccaga agaagccgca ggcgtcgtgc acgatcacgg
      841 tcaccgaagg catggtcgtc aagacgcaat acacctcggc ggtcgctgag aaagcgcaga
      901 acggcgtcat cgagttgctg ctggtgaacc acccgctcga ctgtccggtc tgcgacaagg
      961 gtggggagtg cccgctccag aaccagacga tgaccaacgg ctattccgag tcgcgctttc
     1021 gtgacgtcaa gcgcacctac cccaagccga ttgcgatctc agctcaggta ctgctcgacc
     1081 gcgaacgctg tgtgctgtgc acccggtgca cgcggttcgc gcagcagatc gccggcgacc
     1141 cgttcatcga gatgctcgag cgttcggcgc tcgagcaagt cgcggtctac gaagaccagc
     1201 ctttcaactc atacttctcc ggcaacaccg tgcagatctg cccggtcgga gcgctcaccg
     1261 gtacgcagta ccggttccgt gcgcgtccat tcgacctggt gtcatcgccg agcgtgtgcg
     1321 agcactgtgc gtcggggtgc gggcagcgca acgaccaccg ccgcgggaag atgttgcgtc
     1381 gcctagcggg tgtcgatccg caggtcaacg aggagtggaa ctgcgacaag ggccggtggg
     1441 ccttcagcta cgcgaccgcg cccgatcggc tcgactcacc gctggtgcgc gacgccgacg
     1501 gcgacttggt gcccgcgtcg tggccagagg cgctgcgcgc agccgcagcc gggctggccg
     1561 cagcgcgcaa tggtgcaggc gccggcgtgc tggtcggtgg tcggctcacc gtcgaagatg
     1621 cctacgccta cgccaagttc gcgcgggttg cgctcggtac gaacgacgtc gacttccgcg
     1681 ggcgggtgca ttctgacgaa gagctcgcct tcctcggatc gcgggtcgcg ggccgcttcc
     1741 tcgaggtgac gtacgacgat ctcgaggctg cgtcgaccgt gttgctcgtc gggttcgagc
     1801 cagaagaaga gtcacccatc gtcttcctgc ggttgcgcaa ggcagcgatg aagaagcacg
     1861 ccaaggtcgt gtcgatcgcg cccttcgcca cccgcggcct tgagaagatg cgcggccgcc
     1921 tgatcgccac cgttccaggc gccgaagccg tggtcttgca ggcactcgcg gcgggcgaaa
     1981 cggtcgaggt gccgccgggt gtcgactctc acggatactc cgagttgctc accgaggccg
     2041 ccgccggcct cgcacttccg gcgagcctga tcctggtcgg tgagcggctc gcggccgtcc
     2101 ccggcgggtt gtcggccgcg gtcgcgctgg ctgatgcgac cggcgcccgg ctcgcctgga
     2161 ttccgcgccg agcaggtgag cgcggagccg tcgaggccgg tgcgctgccc aacctgttgc
     2221 caggtggtcg tgcccttgcc gacaacgacg cgcgcggcga ggttgcgctg aactgggcga
     2281 cgtccatctc ggcaaagcct ggccgcgaca cgagcggcat gctcgccgcc gcgaccgacg
     2341 gctcgctggg tgcgctggtt gtggcgggcg tcgatcccga cgaccttccc gacccggcag
     2401 ccgcgcacgc cgctcttgct gccgcgggct tcgttgtcag cctcgagctc cgacgatcag
     2461 ccgtgactga cgtcgccgac gtggtgttcc cggtcgccgc ggccgttgag aaggcgggca
     2521 cgttcatcga ctgggaggga cggtcgcggc cgttcgaggc ttcgttgacc gacagcggtc
     2581 aactgtcgga tctcgaggtg ctgcacgcac tggccgacgc actcgacgtc catctcggat
     2641 tgccggatct gcgtaccgcg cgcgccgagt tgcaggctct ctcagcacac accggttcga
     2701 tggccgcgcc gtcggccccg actgccatgc ccgccccacc tgtgaagttg acgcccggtc
     2761 acgccgtgct ggcgacgtgg cacgagttgc tcgacgaggg cagcttgcaa gacggcactc
     2821 catacctggc cgcaactgca aagccaatac atgcccggct atcgccggcg accgcacggg
     2881 cggtaggagt ggtcgacggc gcgcccgtca cgattcgggc cggcaaggcc ggcggtgcga
     2941 tcacgctcga cctgctcatc accgcgatgc ccgacggagt cgtgtgggtg cccacccgct
     3001 cggtgggcag tgcggtgcac cgtaccctcg gtgtcggcgc cggtgctgtc gtcagtctcg
     3061 aagggggctc ccagtgaacg cgcacgttct gggtgcgcag ctcgtcgcga gtgcggtgag
     3121 cgagccgaac catcgtgacc tgtggtggct catcatccta aaaaccgttg tggtgttcgg
     3181 tttcttgatg gtcatgacgc tgttcatgat ctgggcagag cgccgcgttg tcgcccggat
     3241 gcagcagcgg cttgggccca accgggtcgg ccccttcggt ctgttgcagg gtctcgccga
     3301 cggcatcaag ttggcactga aggaagacct cattcccact gccgctgacc gcatcgtgtt
     3361 catcctggcg ccgatcatct cggcgattcc ggcgttcatg gcgttcgcgg tgatcccgtt
     3421 cggcccaacg gtctcgatct tcggcagcca cacgaacctg caactgaccg acctgccggt
     3481 cgcggtgttg ttcgtgctcg cgatgagttc gatgggcatc tacggcatcg tgcttgccgg
     3541 ctggtcgagt ggctcgacgt acccgttgct cggaggcgtc cgctcggctg cccagatgat
     3601 cagctacgaa gtggcgatgg gcctctcgtt cgtgggcgtc ttcatccagt cggggtcgct
     3661 gtcgacatct gagatcgttg ccgcgcagca gcagcgctgg ttcgtgctgt cggctttcgt
     3721 ctcgttcctc gtttacgcga tctcgatggt gggcgaaacc aaccgtgcgc cgttcgacct
     3781 gcccgaggct gagggcgaac tcgtgggcgg gttccacacc gagtacacgt cgctgaagtt
     3841 cgcgctgttc ttcctcgccg agtacatcaa catggtcacg gtctccgcgc tgatgaccac
     3901 gctgttcctt ggcggttggc gggcaccctg gccgatctcg ttgtggcatg acgccaacac
     3961 cggttggtgg ccgatgctgt ggttcttcgc caagttgttc tgcgtgctct tcgtcttcat
     4021 ctggttgcgt ggcacgctgc cgcggttgcg ctacgaccag ttcatgaagt tcggctggaa
     4081 ggtcctgatc ccggcaagcc tgatctggat catggtcgag gccgccatca aggtgctcga
     4141 ccgcaagcag ctcatctggg gaatcggcgt cacggcgatc ctcgtcatcg cggcgtcgtt
     4201 cgtttacgac agcgtcgagc agaacaagca gcgcgccgtc gaggaagaag aggaagccgc
     4261 gagtcgcgca ccgttcgatc cgtacgcagg tggattcccc gtaccgccga tgccgggtca
     4321 gcagctgcca ctacgccgac ccgtgccgat cctggccgga aacgcgggcg cggcgagtgc
     4381 gccgaccgac gatgacgatg accccagcga ccccaacgac cccaacgaca ctgacccgtc
     4441 tgacccctct ggctctggcg aggtcatcgg tggctagttt cttcgggccg gtgaagggtt
     4501 tcggcgtcac gttcgccacc atgttcaaag cggtcaacac cgagctctac cccgagcaca
     4561 agaagatcac gatgccgcgc ttccacgggc ggcacgtact caacaggcat cccgacggct
     4621 tggagaagtg cgtcggctgt gagctgtgcg catgggcctg cccagccgac gccatctatg
     4681 tcgaaggtgc cgacaacacc gaagaagagc gcttctctcc aggcgagcgg tacggccgcg
     4741 tttaccagat caattacttg cgttgcatct tgtgcggctt atgcatcgag gcctgcccga
     4801 cccgtgcgct cacgatgagc aacgagtacg agatggccga cgacaacaga gccgacctga
     4861 tcttcaccaa ggagcaactg ctcgcacccc ttgctcccgg catggagttg ccgccacacc
     4921 cgatgcgcct cgccgacgcc aaggactact accgaggcac cccggcagcg cacgtcatgt
     4981 tcggtgacga cgacgccccc gaggtgaccg agtaatgcca gcggtcgtcc tcgcttcgag
     5041 catgcatggt ggtggtcccg agaccgccat cttctggatc tttgctccgc tgtgcgtgct
     5101 cgctgcgctc ggcatggtgt tttcgagcaa caccgtgcac ggggcgctgc tgctcggcgg
     5161 cgtcatgctg tcgctcgcgg tcttctacgc ggcgcaagac gcgccgttcc tcgcgttcgt
     5221 gcaggtcatc gtctacaccg gcgcggtgat gatgctgttc ctgtttgtgc tgatgctcat
     5281 cggcgtcgac acccgcgaaa gtctcgtcga gacattgcgt ggccagcggc tcgctgcgtc
     5341 aagcgcggca cttggctttc ttatcttgtt gttcgccggc attcggcagg cttcgatcac
     5401 cacgagcgca gggctggcga gtgcgaacaa tccgaccgac gcgcagggca acgcagtcgc
     5461 gggccaaggc aacatcttcg aaatcgcgag gctcctgttc accaggtaca tctttgcttt
     5521 cgaggtcacg agcgccctgc tcatcacggc agccctaggc gcgatggtgc gcgcgcaccg
     5581 cgaaccgcat gagcccaagc gtggtcagcg cgagcagatg cgcgaccggt tcaagaccgg
     5641 gacccacccg acgccgttgc cggggccggg catctatgcg cgacacaacg cggtcgacac
     5701 tccggcgttg ctacctgacg gcagcccgtc ggagttgagt gtgccgcctg ccgtcgagga
     5761 attgcggctc gagcgcggcc gcaacccgca gccggcccaa ctcggtggca agccaggaac
     5821 gaggcagcca tgagcccgac cta
//
LOCUS       DASWVF010000496         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_472253, whole genome shotgun sequence.
ACCESSION   DASWVF010000496 DASWVF010000000
VERSION     DASWVF010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472253"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VGD55_08860"
     CDS             complement(<1..1382)
                     /locus_tag="VGD55_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0870496.1"
                     /translation="MRKVLIANRGEIAVRIARACRDAGLASVAVYAEPDLDSLHARAA
                     DEAYALGGSTPAESYLSISKVLAVAARSGADAVHPGYGFLAENASFARAVVDAGLIWI
                     GPPASAIDALGDKVQARHIAASVGAPLVPGTAEPVADADEVVSFAREHGLPIAIKAAF
                     GGGGRGLKVARTLEEIPELFASAVREAVTAFVRGECFVERYLDRPRHVETQCLADAHG
                     NVVVISTRDCSLQRRHQKLVEEAPAPFLSADALATLYSSSKAILGAAGYVGAGTCEFL
                     LGADGTISFLEVNTRIQVEHPVSEEVAGIDLIREQFRIADGEELGYDDPPLRGHSIEF
                     RINGEDPGRGFFPAPGTVTAWRPPSGPGVRLDSGVSAGTVVPQAFDSLLAKLIVTGAS
                     RTEALARARRALAEFEIDGMATALPFHRAVVDDPAFAPSDPQTPFSVHTRWIETEFVN
                     TIAPYVSSAG"
     gene            1451..>2330
                     /locus_tag="VGD55_08865"
     CDS             1451..>2330
                     /locus_tag="VGD55_08865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0870497.1"
                     /translation="MRTEWIEAARPRVLTDAARSAALLLFVGAVVITLEATVVPTPQY
                     PHGLRWVPWLVAAVFAVVGVLLWRMPERLPTLCWPLVPAVPVVVILLIALGSRDATAS
                     SQLAFSLPLLFAAYHLKPVVARLVAGEIVIAETILCVLIDPPGRVIEDSVGVSLILVG
                     IMVTLVTARNRFDDAISSLRYEAQHDAVTGLLTRRTFDFDIEQLDDGEPVSLILVDID
                     DFKTVNDTFGHDIGDQTLRIVAKALSANSRQNDRAYRLGGDELAVLLSGTTPETALRR
                     AEVIRRAVETGPTAFVA"
BASE COUNT          388 a          788 c          763 g          391 t
ORIGIN      
        1 tcacctgccg aagaaacgta cggcgcgatg gtgttgacga actcggtctc gatccagcgg
       61 gtgtgcacgc tgaatggggt ctgcgggtct gacggagcga acgcgggatc gtcgaccacc
      121 gcgcgatgaa acggcagcgc agtcgccatt ccgtcgatct cgaactcggc gagtgcgcga
      181 cgcgctcggg cgagtgcctc cgtgcgagat gcgccggtca cgatgagctt cgccagcaac
      241 gagtcgaacg cctgggggac aaccgtgccc gccgagactc cggagtcgag ccgcactccc
      301 ggtcctgaag gcggccgcca ggcagtgacc gtgccgggag cggggaagaa accccgaccc
      361 ggatcctcgc cgttgatacg gaattcgatc gagtgaccgc gcagcggcgg atcgtcgtag
      421 cccagctcct cgccgtccgc gattctgaat tgttcgcgga tcaagtcgat gcctgccact
      481 tcctcagaca ccgggtgctc gacctgaatg cgggtgttca cctcgaggaa cgagatggtg
      541 ccgtccgcgc cgagcaagaa ctcacacgtg cccgcgccga cgtagccggc cgcccccaag
      601 atggccttcg atgacgagta cagggtcgcg agagcatccg ccgaaaggaa cggcgccggc
      661 gcttcctcga ccagcttttg gtggcggcgt tgcaacgagc agtcgcgcgt cgaaatcacc
      721 acgacgttgc cgtgagcatc tgcgaggcat tgcgtctcga cgtgccgcgg ccggtctaag
      781 tagcgctcga cgaagcactc accgcgaacg aacgcggtga cggcctcgcg caccgccgac
      841 gcaaaaagtt cagggatttc ctcgagcgtg cgggccactt tgagtccgcg accgccgccg
      901 ccgaatgccg ccttgatcgc gatcggcaac ccatgctcac gagcgaacga gacgacctcg
      961 tcggcgtcgg cgaccggctc ggcagtgccc ggcacgaggg gagccccgac gctcgcagcg
     1021 atgtgtcgtg cctgaacctt gtcgcccaag gcgtcgattg ccgaggccgg cggcccgatc
     1081 cagatcaacc cagcgtcgac cacggcccgg gcgaacgacg cattttcggc gagaaagccg
     1141 taccccgggt gcacggcgtc tgcccccgag cgagcggcca ctgcaagaac cttggagatc
     1201 gacaggtagc tctccgccgg cgtcgaaccg ccgagcgcat aggcctcgtc agccgcgcgg
     1261 gcatgaagcg agtcgagatc aggctcggcg taaaccgcga cgctcgccaa cccggcgtcc
     1321 cggcatgccc tcgcgatgcg aacggcgatc tcgcccctgt tggcgatgag aaccttgcgc
     1381 acccggcctc cctagcttgc cggggagtct acgtacctca gttcaggtcg tcagcggccg
     1441 atagggcgcc gtgcgaacag agtggatcga ggctgctcgc ccgcgggtgc tgacagacgc
     1501 ggcccggtcg gctgccctcc tcctgttcgt gggtgccgtc gtcatcaccc tcgaggcgac
     1561 ggtcgttccg acgccgcagt atccgcacgg ccttcgctgg gtgccctggt tggtggccgc
     1621 agtcttcgcc gtcgtcggcg tgctgctgtg gcggatgccg gagcggctgc ccacactgtg
     1681 ctggccgttg gtgcctgcgg ttccggtggt cgtcatcttg ctgattgcgc tcggcagccg
     1741 cgatgcgacc gcctcttcgc aactcgcatt ctcgttgccg ttgctcttcg cggcgtacca
     1801 cctcaaaccc gtcgtggcgc gcctggtcgc tggcgagatt gtcatagcgg agacgattct
     1861 gtgcgtgctg atcgacccgc ccgggcgggt catcgaagac tcggtcggcg tgtcgctgat
     1921 cctcgtcggc atcatggtga ccctggtcac ggcccgaaat cgcttcgacg acgcgattag
     1981 ctcacttcgc tacgaggccc agcacgacgc ggtcaccggg ctgttgaccc gtcgcacctt
     2041 cgacttcgat atcgagcagt tggacgacgg tgagccggtg tccctgatcc tcgtcgacat
     2101 cgatgatttc aagaccgtga acgacacgtt cggccacgac atcggcgacc agacccttcg
     2161 cattgtggca aaggcgctct cggccaacag ccgccaaaac gatcgcgcgt accgcctcgg
     2221 gggagacgaa ctcgccgtgc tgctgagtgg caccaccccg gagaccgcac tgcgtcgggc
     2281 agaggtgatc cggcgggctg tcgagaccgg tccaacggcg ttcgtcgccc
//
LOCUS       DASWVF010000497         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_497518, whole genome shotgun sequence.
ACCESSION   DASWVF010000497 DASWVF010000000
VERSION     DASWVF010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497518"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VGD55_08870"
     CDS             <1..532
                     /locus_tag="VGD55_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY0870498.1"
                     /translation="HERGAVCHVTVATAWGCPYEGDIAVGRVVDVAKRAVADGADGLS
                     FGDTTGMATPTRVTELVGETRMSLPAVPLNLHFHNTRGTALANVLAALQLGVADFDAS
                     VGGLGGCPYAPGASGNLASEELVHMVEDMGVATGVDLEALIDVAALAERIVGRELPSQ
                     VLRAGPRTRLTTYEPR"
     gene            532..999
                     /locus_tag="VGD55_08875"
     CDS             532..999
                     /locus_tag="VGD55_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870499.1"
                     /translation="MRRIRGALDGSGAVIGAAVFFFALGLILIPVVFLSSVRWTGTSV
                     QGVARGGIVYYSYDGQNYSLDDTSRFDSHTVYFRPSRPDTTAQLGNSAATVVDIASVA
                     VPFLIALAIVGMETRRLLRTRRTRNDPAAIGFGHGLDLEVVHRLLDERRREQS"
     gene            complement(1002..1874)
                     /locus_tag="VGD55_08880"
     CDS             complement(1002..1874)
                     /locus_tag="VGD55_08880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870500.1"
                     /translation="MAAENPQRLRQLLDSGTEAAWSIAAIAVALTPSSPVDVARAARE
                     VVAAVGIDPADHLDRPGIAAEAAAALLQTAALVGGGGASWADQPDEALIAQGRSSAQG
                     AAMLKTHGVPMMQGLGDALSRPGARMLDVGTGVAALAVAWAELFPALTVVGLDVMPRV
                     LALAANNVAASTVADRVILREQDIGTLDDEGEYVLAWLPAPFVPEPALRAGVVRIARA
                     LTPGGWAMVAHSKYGGDPIEDALSRFKTAAYGGTALNADQAQSLLRDAGLGDVMTMPT
                     PQGAPGITVGRKGL"
     gene            complement(1898..>2840)
                     /locus_tag="VGD55_08885"
     CDS             complement(1898..>2840)
                     /locus_tag="VGD55_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870501.1"
                     /translation="TGLVFAVAFLTGDKHVLVAVNAETGALAWTRPVDPPGSHSNVEQ
                     ERGALLLAGGRVWVPYGGLDGDCGPYHGYVVGITTGGQGTASIYQVPSSREAGIWTPA
                     GPAADAAGHVYVSVGNGAQTDGNAKYDLSDSVIELNGTSVVSFFAPATWASENAADLD
                     LGTTGPVILPTGQVFVAGKNGNAYLLKQGALGGLGGKVPSIAICTAFGGAAYTAGTIY
                     IPCTNGVRAVHISGSTMTPVWHASPAGSPIIGGGAVLAVAQSAGVLYALDPTTGAVKS
                     QVALGDPTTRFATPALAADGFAYVGTQHGDLVIVATS"
BASE COUNT          473 a          948 c          925 g          494 t
ORIGIN      
        1 gcacgagcgc ggcgccgtct gccacgtgac tgtcgcaacg gcgtgggggt gcccctatga
       61 gggtgacatc gcggtcggcc gcgtcgtaga cgtcgccaag agagctgtgg cggacggcgc
      121 cgacgggttg tcgttcggcg atacgaccgg catggcaacc ccgacgcgag tcaccgaact
      181 ggtgggcgag actcgcatgt cgttgcccgc tgttccgctg aacctgcact ttcacaacac
      241 gcggggtacc gcgctggcga acgtgttggc ggcgttacaa ctgggtgttg ccgatttcga
      301 cgcgagcgtt ggtggccttg gcggatgtcc ttacgcgcca ggtgcttctg ggaatctcgc
      361 gagcgaagaa ctcgtgcaca tggtcgaaga catgggggtc gcgacgggtg tcgatctgga
      421 ggcgctgatc gatgtcgcgg ccctggccga acgcatcgtc ggtcgcgagc tgccgtcaca
      481 ggtgctgcgc gccggcccgc gtacgcgtct cactacctat gagccacgtt gatgcgtcga
      541 atcagagggg cgctagacgg cagcggcgct gtcatcggtg ctgcggtgtt cttcttcgcg
      601 ctcgggctca tccttatccc cgtcgtcttc ctctcgtccg tgcggtggac ggggacctca
      661 gtgcaaggtg tcgcccgcgg cggcatcgtc tattacagct acgacggcca gaactacagc
      721 ctcgatgaca cgtcccgctt cgactcgcac acggtgtact tcagacccag ccgcccagac
      781 acgacagccc aactcggcaa cagcgcagcg acggttgtcg acatagcaag cgtggccgtc
      841 ccgtttctta ttgcgctggc catcgtcggc atggaaacac gccgcctctt gcgcacccgc
      901 cgcacgcgaa acgacccggc agccatcggc ttcgggcacg ggctagacct tgaggttgtg
      961 caccgactgc ttgacgagcg tcgtcgcgaa caaagctgac ctcaaagacc cttccggccg
     1021 accgtgatgc ccggagcgcc ttgcggcgtg ggcatcgtca tgacgtcgcc gaggccggcg
     1081 tctcgcagca gtgattgggc ttggtcggcg ttgagcgcgg tgccgccata cgccgcagtc
     1141 ttgaaccttg agagcgcgtc ctcgatgggg tccccgccgt acttgctatg cgcgaccatc
     1201 gcccaaccgc ccggcgttag cgcgcgtgcg atccgcacga cacctgcacg taacgcaggt
     1261 tctggcacga acggggccgg caaccacgca agcacatatt cgccttcgtc atcgagagtg
     1321 ccaatatctt gctcgcgcag gatgacacgg tctgcgaccg tgctcgcagc aacgttgttc
     1381 gcagccaatg ccagcactcg cggcatgaca tcgaggccga caaccgtcag ggccggaaag
     1441 agctcggccc aggcgaccgc cagcgcggcc acccctgtgc cgacgtcgag catccgtgcg
     1501 cctgggcggc tgagcgcatc acccagcccc tgcatcatcg gaactccgtg tgtcttgagc
     1561 atcgccgcgc cctgagcact cgagcgtccc tgcgcgatca acgcttcgtc tggttgatct
     1621 gcccacgatg cgccgccgcc gccgacaagg gcggcagtct gcagaagtgc tgcggccgcc
     1681 tcggcggcga tgcctgggcg atcgaggtga tccgccgggt cgattccgac cgcagcgaca
     1741 acctctctgg cggcgcgggc gacgtcaacg gggctgcttg gcgtgagggc tacggcgatt
     1801 gcggcgatcg accacgctgc ttcggtgcct gagtcgagca actgtctcaa gcgctgcggg
     1861 ttttcagcgg ccacgctcta cctccaaggt gcaaacgtca tgatgtcgcg acgatcacca
     1921 aatcaccatg ctgggtgccg acgtaggcga aaccgtcggc ggccagtgcc ggcgtcgcga
     1981 atcgggtggt ggggtcgcca agtgcgacct gcgacttcac cgcacccgtc gtcgggtcga
     2041 gcgcatacaa aacgccggcg gattgcgcca cggcaagtac cgctccgccg ccgatgatcg
     2101 gcgatccggc cggcgatgcg tgccagaccg gcgtcatcgt cgaccctgag atatgcacgg
     2161 ctctcacgcc gttggtgcac gggatgtaga tcgtcccagc tgtgtacgca gcgcctccaa
     2221 acgcggtgca gatcgcaatg ctcggcacct tgccaccgag accgccaagc gcaccttgct
     2281 tcaacaaata ggcgttgccg ttcttgccgg cgacgaagac ctgacctgtc ggcaggatca
     2341 ccggcccggt cgtaccaagg tcgaggtcgg ccgcgttctc acttgcccac gtcgcaggag
     2401 caaagaacga gacgacgctg gtgccgttga gttcgatgac cgagtcactc aggtcgtact
     2461 ttgcgttgcc gtcggtctgc gcgccgttgc caaccgagac gtacacatgt ccggccgcgt
     2521 cggcggccgg tccggcgggt gtccagatgc cggcctcgcg tgagctcggc acttggtaga
     2581 tcgatgcggt gccctgacct ccggtggtga tgcccacgac gtacccgtga tacggcccgc
     2641 agtcgccgtc caacccgcca tatggcaccc acacgcgacc gccggcgagc agcagcgctc
     2701 cccgttcctg ctcgacgttg gagtgcgaac caggagggtc gaccggtcgg gtccaggcga
     2761 gcgcgccggt ctcggcattg accgcgacta gcacgtgctt gtcgccggtc aggaacgcga
     2821 cagcgaagac gaggccggtc
//
LOCUS       DASWVF010000498         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_140965, whole genome shotgun sequence.
ACCESSION   DASWVF010000498 DASWVF010000000
VERSION     DASWVF010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140965"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGD55_08890"
     CDS             complement(<1..270)
                     /locus_tag="VGD55_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0870502.1"
                     /translation="MPVRSHTLIDVQTPTGLAQADLERPATAPTGLLLLGHGAGGSIE
                     APDLRAMTAAARGAGLAVARVRQPYSVAGRRAPAPAAQLDAAWTAV"
     gene            complement(272..1027)
                     /locus_tag="VGD55_08895"
     CDS             complement(272..1027)
                     /locus_tag="VGD55_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY0870503.1"
                     /translation="MCGRYATARDPADLIEEFGVDRDLVDEQLAPNYNVAPTDPVYAV
                     VERLNKEQPSEGPQRQLRVVRWGLVPSWAKDRKIASKLINARSETLSEKPAFRRAFAT
                     RRCLLPADGYYEWYTDDPADSAADTPRGKLPKQPYFIRNKGGGVLPMAGLYEFWRDPA
                     AEPDVDPWLVSSTVITTEATDDIGRIHDRMPMFVEPENWARWLDPRIDDLEMLGSLLM
                     PAAAGRLEAYPVSTQVNDVRNNGAQLIEPLSGA"
     gene            1073..>1769
                     /locus_tag="VGD55_08900"
     CDS             1073..>1769
                     /locus_tag="VGD55_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870504.1"
                     /translation="MSEPEPSRLPAAVLWPAPTASKPVDATIALPGSKSITNRALPLA
                     ALADSPTIVRGALRSRDTDLMAAGLRALGADIDDSGDDWSVTPAALRGPADIDCGLAG
                     TVARFLVAISSLARGPVRFDGDPRARERPMRPLLDGLRQLGLRVDDDGTHSLPLTVFG
                     TGELPGGQCVIDSSSSSQFVSALLLAAPRATGAIVVRHVGSILPSLPHIEMTVAMLRS
                     RGVVVDDSQPNTWQ"
BASE COUNT          267 a          603 c          597 g          302 t
ORIGIN      
        1 aaccgcggtc cacgcggcgt cgagctgggc agcaggcgcc ggagcgcgac gaccggcaac
       61 cgagtaaggc tgacgcacgc gggccaccgc gagtccggcc ccgcgcgccg ccgcggtcat
      121 cgcgcgcagg tcaggcgcct cgatgctgcc gcctgcgccg tggccgagca gcaacaagcc
      181 ggtcggtgcg gtggccgggc gttcgaggtc agcctgcgca agacccgtcg gggtctgtac
      241 gtcgatcagc gtgtggctcc taacaggcac gtcatgcacc cgatagcggc tcgatcagct
      301 gcgcgccgtt attgcgcacg tcgttgacct gcgtcgatac cggatacgcc tcgagcctgc
      361 cagcggcagc gggcatcagg agcgagccga gcatctcgag gtcgtcgatg cgcgggtcga
      421 gccagcgcgc ccagttctcc ggctcgacga acatcggcat ccggtcatgg atgcggccga
      481 tgtcgtcggt ggcctcggtg gtgatcaccg tcgatgacac aagccacgga tcgacgtccg
      541 gctccgccgc gggatcgcgc cagaactcgt agagccccgc catgggcagc acgccgccgc
      601 ctttgttgcg aatgaagtag ggctgcttgg gaagctttcc gcgcggggta tcggccgccg
      661 agtctgccgg gtcgtctgtg taccactcgt aatacccgtc ggcgggcagc aggcagcgtc
      721 gcgtcgcgaa tgctctgcga aacgctggct tctccgagag cgtctccgac ctggcgttga
      781 tgagcttgct ggcgatcttg cgatccttcg cccacgaggg aacaagcccc catcggacga
      841 cgcgcagctg gcgttgtggc ccctcgctcg gctgctcctt gttaagacgc tcgacgactg
      901 cgtacaccgg gtcggtcggc gcgacgttgt agttaggcgc caactgctcg tcgaccaggt
      961 cgcggtcgac cccgaattcc tcgatgagat cggccgggtc gcgcgccgtc gcatagcgtc
     1021 cgcacatccc tgcaggatgc caccccgtcg agcaccccgg taagttcgtg gcgtgagcga
     1081 acccgaacca tcgcggctgc cagcggcagt tctgtggcct gcgccgacgg cgtcgaaacc
     1141 ggtcgacgcg accatcgcgc tgccgggttc gaaatccatc actaacagag cgttgccact
     1201 agctgcgctt gcggacagcc cgacgatcgt gcgcggcgcg ctgaggtcgc gcgatacaga
     1261 tctcatggca gcagggttgc gcgcgctagg tgccgacatc gacgactcgg gcgacgactg
     1321 gtctgtcaca cccgctgcgc tgcgcggccc ggccgatatc gactgcggcc tggcgggcac
     1381 cgtcgcccga ttcctcgtcg cgatttcgtc gctcgcccga ggaccggtgc gcttcgacgg
     1441 cgatccgcgt gctcgcgagc gtccgatgcg gccgcttctc gatgggttgc ggcaactcgg
     1501 cctccgagtc gacgacgacg gcacgcactc gctgccgctg accgtgttcg gcactggaga
     1561 gttgccgggc ggccagtgcg ttatcgactc gtcgagctcg agccagttcg tgtcggcact
     1621 cttacttgca gcgccacggg caaccggcgc gatagtcgtg cgccatgtcg ggtccatcct
     1681 gccgtcactt ccgcacatcg agatgacggt cgcgatgttg cggtctcgcg gtgtcgtcgt
     1741 cgatgacagc cagcccaaca cctggcagg
//
LOCUS       DASWVF010000499         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_191798, whole genome shotgun sequence.
ACCESSION   DASWVF010000499 DASWVF010000000
VERSION     DASWVF010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191798"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..824
                     /locus_tag="VGD55_08905"
     CDS             69..824
                     /locus_tag="VGD55_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0870505.1"
                     /translation="MDTEPYTELDPLEPPAAEVPAAPAPTGLRRDQVYGELRKRLMLG
                     EFPVNSRLVEERLAQVLGVSRTPVREALVRVLADGLVNRVDGGYYVALPDFTGLRDLY
                     DLRITLELRGISRALESDAVRHDLALLEPLRDSWRGLWDDRPEPNPDFVSVDEDFHVT
                     LLKSSGNAALAQTLVSINSRIRPVRMYDYMTEKRIELTISQHLGILEAVLNTRMDDAL
                     LALRRHVGESLEVVERRAARAITAMALRRAGVS"
     gene            821..>2286
                     /locus_tag="VGD55_08910"
     CDS             821..>2286
                     /locus_tag="VGD55_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870506.1"
                     /translation="MIDLDAPLLEVKDLTKRFGALVANDRVGFELPAGQVHALLGENG
                     AGKSTLVKMLYGVYKPDGGVIKRDGREVTITSPATARDLGIGMVFQDLRLVPALTVWE
                     NVALHIGGQRILNAGKVQSDIAEASERYGLAVNPKARVADLSIGEWQRVELVKVLLGG
                     AKVLILDEPTSVLTPTEVDGLFTVVRQLRSEGVGIVIITHKMREVRDIADQVTVLRAG
                     RVVFANTPEGDLSDDALATAMVGETVVPVRNERSGDAESVPPLLKASGISLTGLGGGP
                     GLRHVDLELWAGEILGIAGVAGNGQRELTDVLSGAAQSESGELIVGGVSMTHANPARF
                     HRAGVVAVVADPVRQFVVPGLSVAAHAALWASSQHGGGLHFDVKVAARKLRADGDRLG
                     LRPADPSRQLDRLSGGNIQRVLLTLALGEPAKILVVSYPTRGLDVLTTETTRALLLAA
                     RDSGTAVVLISEDLDELLALSDRIAVLAHGRIAGTVAGG"
BASE COUNT          376 a          735 c          759 g          416 t
ORIGIN      
        1 ggccacgcaa cacgaaagtt acgttcgata cacgggtcgg caatgtcgac tgcctagcgt
       61 tccgcgccat ggacacggaa ccgtatacag agctggatcc tctcgagccg ccggccgcgg
      121 aggttcccgc ggcgcctgca ccgaccggtc tacgccgcga ccaggtctac ggtgagctgc
      181 gcaaacggct gatgctcggc gagttcccgg tcaacagccg cctcgtggag gagcggctgg
      241 cccaggtgct cggggtctcc cgcacgcctg tacgggaggc gctcgttcgg gtgcttgcgg
      301 acgggctggt taaccgggtt gacggcggct actacgtcgc gctgcccgac ttcacggggc
      361 ttcgagatct ttacgacctg cgtatcaccc tggagctgcg cgggatctcg cgcgctctgg
      421 agtctgatgc tgtccgccac gacctcgcac tgctcgaacc gctgcgcgac tcctggcgag
      481 gcctgtggga cgaccgaccg gagcccaacc ccgatttcgt ctccgtcgac gaggacttcc
      541 acgtcacctt gttgaagtcg tcaggaaacg cggcgctcgc gcagacgctc gtctcgatca
      601 actcacgcat tcgaccggtc cgcatgtacg actacatgac tgagaagcgc atcgaactca
      661 cgatctcgca gcacctgggg atcctcgaag cagtgttgaa cacccggatg gacgacgcgt
      721 tgctggcgtt acgccgtcac gtcggcgaat ccttagaagt tgtcgagcgt cgagccgcgc
      781 gggcgatcac ggcgatggcg ttgcgaaggg caggggtctc atgattgacc tcgatgctcc
      841 gctgcttgag gtgaaggacc tcaccaaacg atttggtgcg ttggtcgcca acgatcgcgt
      901 cggcttcgag ttgccggccg gccaggtgca cgcgctgctc ggagaaaacg gcgcaggtaa
      961 gagcacgctg gtcaagatgc tctatggcgt ttacaagccc gacggcggcg tcatcaagcg
     1021 cgacggtcgc gaggtaacca tcacctctcc cgctaccgcg cgcgacctcg gcatcggcat
     1081 ggtgttccag gatctgcggc tcgtcccggc gctgaccgtc tgggagaacg tggcattgca
     1141 tatcggtggc cagcggattc tcaacgcggg caaggtgcaa agcgacattg ccgaggcgtc
     1201 cgagcgctac ggcctcgccg tgaaccccaa ggcgcgagtc gccgatctct ccatcggtga
     1261 gtggcagcgg gtcgagctgg tcaaggtgct gttgggtggc gccaaggtat taatccttga
     1321 cgagccgacg agcgttttga cccccaccga ggtcgacggt ctgttcaccg ttgtgcgcca
     1381 actgcgatct gaaggcgtcg gcatcgtcat catcacccac aagatgcgcg aggtgcgcga
     1441 catcgccgat caggtgaccg tgttgcgtgc aggcagggtt gtctttgcca acactcccga
     1501 gggcgacctc agcgacgatg cgctggccac cgcgatggtc ggcgagaccg tcgtgcccgt
     1561 gcgaaacgag cgatctggtg acgcagaatc ggtcccgcct ctcttgaagg cgagcgggat
     1621 ctcgcttact ggcctcggcg gtggcccggg cctgcggcac gtcgacctcg aactgtgggc
     1681 gggggagatt ctcggaatcg ccggagtcgc cggcaacggg caacgtgaac tcactgacgt
     1741 gttatccggc gctgcgcagt cggagagcgg cgagctgatc gtcggcggcg tttcgatgac
     1801 ccacgcgaac ccggcgcgtt ttcaccgcgc gggcgtggtg gctgtcgtcg ccgacccggt
     1861 taggcagttc gtcgtccctg gcctcagcgt cgctgcgcat gcggccctgt gggcgagttc
     1921 gcagcacggc ggtgggttgc acttcgacgt caaggtggcg gctcgaaagc tgcgtgcgga
     1981 cggcgaccgg ctgggcttgc gcccagctga cccgtcgcgc caactggatc gactctccgg
     2041 tggcaatatc caacgtgtcc tgctcacgct cgcactcggt gagccggcca agattctcgt
     2101 cgtctcctac cccacacgcg gtctggacgt gctcacgaca gagacgacgc gcgcgctcct
     2161 gctggccgcc cgcgactcag gaacagcggt ggtgctcatc tccgaggatc tcgacgaact
     2221 gcttgccctt tcggaccgca tcgctgtgct cgcgcacgga cggatcgccg gcacggtcgc
     2281 cggcgg
//
LOCUS       DASWVF010000500         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_472334, whole genome shotgun sequence.
ACCESSION   DASWVF010000500 DASWVF010000000
VERSION     DASWVF010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472334"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGD55_08915"
     CDS             complement(<1..260)
                     /locus_tag="VGD55_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870507.1"
                     /translation="MRTQLAPGTADHVVEGIRIRVTTYGSPGDGLDSSPAVLLLHGFP
                     TSSQLWRQVGPEMCAGTDDDLFVLAPDLVQLGESERTGCRIDL"
     gene            347..1576
                     /gene="ilvA"
                     /locus_tag="VGD55_08920"
     CDS             347..1576
                     /gene="ilvA"
                     /locus_tag="VGD55_08920"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0870508.1"
                     /translation="MTVALPRDPQEWVADARAAAALLDGVARRTPIESSRGLAELVGG
                     DVLLKCENLQRAGSFKIRGAYVRIARLTDDERARGVVAASAGNHAQGVALAASLLGAK
                     STVFMPEGAPLPKVEATRKYGADVRFFGHTLDEALAEAISFAAATGAMLIHPFDHPDI
                     VAGQATVGLEILEQRPDARTIVVSTGGGGLLSGVAVVVNALRPDIRVVAVQAQGAAAF
                     PGSLEAGTPTRLASMSTIADGIAVGCPGDLTFELVRDYVDRVVTVSEDSLSRALLFCL
                     ERAKLVVEPAGVAAVAAIIDAPEMFEPPVVAVLSGGNVDPVLLMGVLRHGLAAAGRYL
                     SFQVTISDRPGALAALLQALAAAGGNVLDVVHQRTAPTLRLDEVAVALQVETRGPTHS
                     DEVVDMLRSQGYRLEFT"
     gene            complement(1669..>1900)
                     /locus_tag="VGD55_08925"
     CDS             complement(1669..>1900)
                     /locus_tag="VGD55_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870509.1"
                     /translation="PRGPAALLRPPPRLLLRPLPDLPLPALLLTLLVPRLRRTSATPS
                     VISNAAAKSGTRGNDVTCERAWCTASGFRGDD"
BASE COUNT          291 a          613 c          663 g          333 t
ORIGIN      
        1 agatcgatac ggcagccggt gcgctcggat tcgccgagtt gcaccaagtc gggtgccagc
       61 acgaagaggt cgtcgtcggt accggcgcac atctccggac cgacctggcg ccacaactgt
      121 gacgacgtgg ggaagccgtg cagcagcaat accgcaggcg aactgtcgag tccgtcaccg
      181 gggctgccgt aggtcgtgac ccggatccgg atgccctcga cgacgtggtc ggccgtgccc
      241 ggagccagtt gggtgcgcat ccgttcatcc tgcaccggat cgggacgccg aaaccggctg
      301 gggcggcgca atctttttct aaggccatcg gtgcgagact tcgggcatga cagtcgcact
      361 gccacgcgat ccgcaggagt gggtcgccga tgcgcgtgcg gctgcggcgc tactcgacgg
      421 ggtcgctcgc cgcacgccga tcgaatcgtc gcgcggtctc gctgaattgg tcggcggcga
      481 cgtgctgttg aaatgcgaga acttgcaacg cgccggatcg ttcaagattc gcggtgccta
      541 cgttcgcatt gcgcgtttga cggacgacga gagggcgcgc ggtgtggtcg ccgcgagtgc
      601 cggcaatcac gcgcagggtg tcgcgctcgc cgcatcgttg ctcggcgcca agtcgacagt
      661 cttcatgccc gaaggtgcgc cgctgccgaa ggtcgaggcc acccgcaagt acggcgccga
      721 tgtgcggttc ttcggccaca ccctcgatga ggcgttggcg gaggccatct catttgcggc
      781 tgccacgggc gccatgctga ttcacccgtt cgatcacccc gacatcgtgg ccggccaggc
      841 gactgtgggt ctggagatcc tcgagcaacg tcccgatgcg cggaccatcg ttgtcagcac
      901 cggtggcggc gggttgcttt caggtgtcgc cgtcgtggtc aacgccctgc ggcccgacat
      961 cagggtcgtc gcggtgcaag ctcaaggtgc tgcagcattc cccggttcgc tcgaggccgg
     1021 gacgccgacc aggctcgcga gcatgtcgac aatcgctgac ggcatcgcgg tcggatgccc
     1081 cggtgacttg acgttcgaac tcgtccgcga ctacgtcgac cgcgtcgtga cggtgtctga
     1141 ggattcgctg tcgcgggctc tcttgttttg tctcgaacgc gcgaagctgg ttgtcgagcc
     1201 tgccggagtc gccgcagttg ccgcgatcat cgacgcccca gagatgttcg agccgccggt
     1261 ggtcgcggtg ctgtcgggcg gcaatgtcga tccggtcctg ttgatgggcg tgctgcggca
     1321 cggtctcgcc gccgcgggcc gctacttgtc gttccaagtc acgatcagcg accggcccgg
     1381 cgcactcgcc gccctgctgc aggcgctcgc ggccgcgggc gggaacgtgc ttgatgtggt
     1441 gcaccaacgc accgcgccga cactgcgcct cgacgaggtc gcggtggcgc tgcaggtcga
     1501 aacgaggggt ccgacgcact cagacgaggt cgtggacatg ctccggtcgc agggctaccg
     1561 cctcgagttc acctagcgaa gttcacccgg gtgacgtaac cgaaagcttc aggtcgccgt
     1621 ccggtggggt cgaaacaagt agatgaatgc ccgaatctgg gtgctcgctc agtcgtcccc
     1681 ccgaaagccg gatgccgtgc accacgccct ttcgcacgtg acgtcattgc cccgagtgcc
     1741 gctcttggcg gcggcgttgc tgatcacgct cggcgtcgca cttgttctgc ggagccgcgg
     1801 caccaacagg gtcagcagca gggccggcaa cggcaggtca ggcagcggtc ggagcagcag
     1861 tcgaggcggc ggccgtagaa gcgcggccgg accccggggc
//
LOCUS       DASWVF010000501         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_421270, whole genome shotgun sequence.
ACCESSION   DASWVF010000501 DASWVF010000000
VERSION     DASWVF010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421270"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGD55_08930"
     CDS             <1..732
                     /locus_tag="VGD55_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0870510.1"
                     /translation="EAIGQFDLFGSADDAEGLAVGLGFEIVIPSTEWDKRALLAAERD
                     MLGLYVSDHPLFGVEHVLSRLVDCSISSLTTDERAEGGIITIGGIVSGLQRKMTRQGS
                     PWAIATVEDLEGAIEVLFFPQTYQQYAMSLAEDAVICVKGRLSRRDDIPSLHAIELFL
                     PDIGENAQRGPVVLQMPAARCTPPVVERLREVLATHPGVTEVQLQLRNGARTTVLRLD
                     ETLRVDATPALMGDLKALLGPSCLV"
     gene            889..1164
                     /locus_tag="VGD55_08935"
     CDS             889..1164
                     /locus_tag="VGD55_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0870511.1"
                     /translation="MLGLIISLIVIGLVAGALARLAVPGKQNLTVGGTIVLGIVGSFV
                     GGFLGYLLFHHNSQNGFIQPSGIVGSVIGSIIVLLLWLRFGNKPARR"
     gene            1256..2236
                     /locus_tag="VGD55_08940"
     CDS             1256..2236
                     /locus_tag="VGD55_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870512.1"
                     /translation="MAEPVNAGLGCAYAVTGLTKVYRSKSGEVTATDGIDLLVRRGEV
                     FGLLGPNGAGKTSLVRQLIGLLRPDSGRIELFGHDVVAAPRVVPDFVAYLPQDDAALA
                     DLSPADALETTARLRGLDRRDALSARDAMIDELDLSSFASRPISKLSGGQRRLACVGA
                     ALVGDRPVLVLDEPTTGLDPTARRAVWSAIERRRLEAATTVVLVTHNVLEAETVLDRV
                     AILDRGRVIASDTPGRLKAMVDRELRLELAWRHDPPDDDLLVSSLRARAVEHGRRWSV
                     RLPADEARAALAQLSNGPLFAVLDDFTLSTPSLEDVYLTLGGAARDLERT"
     gene            2233..3006
                     /locus_tag="VGD55_08945"
     CDS             2233..3006
                     /locus_tag="VGD55_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870513.1"
                     /translation="MSALPAPAPRPSFGATYAGVLKGQLARAKIAAIPIFLAATLQSV
                     GLVVLLKGLVDSHSDVTEQSVVAGSSVTVVSFVALNLLAQRFGVLKASGALDYFGALP
                     VRPSAIVTATATAWASFTIPGSVLVAVGGAAFYRLPLSHVWVIAPVVVAAGVSLAGLG
                     ALIGLLAPRPEIATVAGQLGMSLVMFLGLVPAHRLPGVVQAVRAVVPSTYAVDAFAES
                     FKATPSWTTISADLAICVGVCVLALTASAWAFRRAVRSR"
     gene            3030..3725
                     /locus_tag="VGD55_08950"
     CDS             3030..3725
                     /locus_tag="VGD55_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870514.1"
                     /translation="MSDDSTDPRSNGSAPEPSEPTLAYPQPRPIPYGSSPYAVTVTTT
                     NPVPDVRQQPLLELADLWTVILGTICVIPLGVVAGFSWLWLAPRAMAVADGKGKTGLL
                     DPGTKAFAGADVTFLFITIVAGLLCGVVAAIIARHRGVAVSVALAAGGTTAAVLAAWI
                     GRTMSGGPAKHWASHVAAGNHRYFLELATRQYLVAWPLAALAVTFVVALLTPDRPVEI
                     VPTEPAPAESGPS"
     gene            complement(3732..4208)
                     /gene="ybaK"
                     /locus_tag="VGD55_08955"
     CDS             complement(3732..4208)
                     /gene="ybaK"
                     /locus_tag="VGD55_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY0870515.1"
                     /translation="MTNGTPALVVLARAGVGHTVHPYDHDPSIRSFGDEAAKALGVST
                     SRIFKTLLVDVDGQLVVAVVPVSGALDLKALAEATGGKRAAMADVAVAERTTGYVVGG
                     ISPLGQRRKLPTVIDASALDHPTIYVSAGRRGLDVELAARDLVAMTGATLAAIARD"
     gene            4695..>5346
                     /locus_tag="VGD55_08960"
     CDS             4695..>5346
                     /locus_tag="VGD55_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0870516.1"
                     /translation="MLDLAMAQPAPADRTFAELGLPKALVMALSKRGIESPFAIQSHA
                     IPDALAGRDVLGRAQTGGGKTMAFGLPLLARLATSGSMRMSKAPRALVLVPTRELAMQ
                     VADALRPLGEALGLKLCCVFGGAPYGKQIDALNRGVDLVVATPGRLIDLLERRCLVLS
                     AVEVAVIDEADHMADLGFLPAVTTILDQTAPDGQRMLFSATLDRGVDKLVRAYLKDP"
BASE COUNT          773 a         1870 c         1766 g          937 t
ORIGIN      
        1 gaagcgatcg ggcagttcga ccttttcggt tcggccgacg acgccgaagg cctggccgtc
       61 ggcttgggct tcgagatcgt gatcccctcg accgagtggg acaagagagc gctgctggcc
      121 gccgagcgcg acatgctcgg gctctacgtc tctgaccacc cgttgttcgg cgtcgagcac
      181 gtgttgtcgc ggctcgtcga ctgttcgatc agttcgctca caaccgacga gcgcgccgaa
      241 ggcggcatca tcacgatcgg tgggatcgtg tcgggtctgc aacggaagat gaccaggcag
      301 ggcagcccat gggcgatcgc gaccgtcgag gatctcgaag gcgcgatcga ggtgctgttc
      361 ttcccccaga cctaccagca gtacgcgatg tcactcgcag aagacgcggt gatctgcgtc
      421 aaaggcaggc ttagccgccg tgacgacatc ccgagcctgc acgcgatcga gctgttcctt
      481 cccgacatcg gcgagaacgc gcagcgcgga ccagtcgtcc ttcagatgcc agccgctcgc
      541 tgcacgccgc cggtcgtcga acggctgcgc gaggtacttg cgacccatcc tggggttacc
      601 gaggtgcagc tccagctgcg caacggggcg cgcacgaccg tgttgcgact cgacgagacc
      661 ctgcgcgtcg atgcaacacc cgcgctgatg ggggacctga aggcattgct cggcccgtcc
      721 tgcctcgtct gacgctgccg cctccacccc tcagatggca ggaggaaatt gatgcatgat
      781 ggaggcatgt caactgtcgg acgccgttcc atcttcgcag cgctcagcct cgcggtcgcg
      841 cgcttcctcc gccgccgccg caagcgccgc ctctaaagga agggaaccat gctcggcctg
      901 atcatcagtc tcatcgtcat cggtctcgtg gccggcgctc ttgcccgact cgccgtcccc
      961 ggcaagcaga acctcactgt cggcgggacg atcgtgctcg gcatcgtcgg atcgttcgtc
     1021 ggcgggttcc tcggctactt gctcttccac cacaactcgc agaacgggtt catccagccg
     1081 tccggcatcg tcgggtcggt catcggctcg atcatcgtgc tgctgctgtg gcttcggttc
     1141 ggcaacaagc ccgcgcgccg gtaagtgcgg cgataggcgc gcggcagggc cgaccgccga
     1201 tccgcgcccg tcggcccgta gcctgctcga cgagatgacg gccgaggagc aagtgttggc
     1261 cgagccggtc aatgccgggc ttggctgcgc ttacgcggtc accggcctca cgaaggtcta
     1321 ccgcagcaag tccggcgagg tgaccgccac cgacggcatc gatctcctgg tcaggcgcgg
     1381 cgaggtgttc ggcctgctgg gtccgaacgg tgcaggcaag acgagtctgg tcaggcagtt
     1441 gatcggcctg ctgcgaccag actccggccg catcgaattg ttcggtcacg acgtggtggc
     1501 ggcgccccgc gtcgtccccg atttcgttgc ctacctgccg caagacgatg ccgcgctcgc
     1561 cgatctctcg ccagccgatg cgcttgagac gaccgcgcgg ttgcgcgggc tcgaccgccg
     1621 cgatgccctc agcgcgcggg acgcgatgat cgacgaactc gacctctcga gctttgcgag
     1681 ccgtccgatc agcaagctgt cgggtggtca gcggcggttg gcctgcgtcg gtgcggcgtt
     1741 ggtcggtgat cgcccggtcc tggtgctcga cgagccgacg accgggctcg atccaacagc
     1801 gcgtcgagcg gtgtggtcgg cgatcgagcg tcgccgcttg gaggccgcga ccacggtggt
     1861 gctcgtcacg cacaacgtgc tcgaggccga aacggtgctc gaccgcgtcg cgatcctcga
     1921 ccgtggccgg gtgatcgcga gcgatactcc cggccggctg aaggcaatgg tcgatcgcga
     1981 gttgcggctc gagcttgcgt ggcgccatga tccgccggac gacgacctgc tggtgtcgag
     2041 cttgcgcgct cgcgcagtcg aacacggccg tcgctggagt gttcggctgc cggccgacga
     2101 ggcccgcgct gccttggcgc agctgtcgaa cgggccgctc ttcgcggtgc tcgacgactt
     2161 cacgctgtcg acaccgtcgc tcgaggatgt gtacctcacc ctcggcggcg cagcacgcga
     2221 tctcgagcgc acgtgagcgc gctccccgcg cccgcaccgc ggccgtcgtt tggcgcgacg
     2281 tacgcgggcg tgctcaaagg ccaactcgcc cgcgcgaaaa tcgccgcgat ccccatcttc
     2341 ttggccgcca ccttgcagtc ggtcggcctc gtggttctgc tgaagggcct tgtcgacagc
     2401 cacagcgacg tgaccgagca gtccgtcgtc gcgggatcgt cggtcactgt cgtctcgttc
     2461 gtcgcactca acctgctcgc gcagcgcttc ggcgtgctga aggcatcggg cgcgctcgac
     2521 tacttcggcg cattgccggt gcgtcccagc gcgatcgtca ccgcgaccgc taccgcgtgg
     2581 gcgagtttca cgatcccggg atcagtgctc gtggcagtgg gcggtgcggc gttctaccgg
     2641 ttgccgctgt cgcacgtctg ggtgatcgcc ccggtcgtcg tagcggcggg agtgtcactg
     2701 gcaggtctcg gtgcgttgat cggccttctc gcacctcgac ccgaaatcgc cacggtcgcg
     2761 ggccaactcg gcatgtcgtt ggtcatgttc ctcggcctgg tgccggccca ccggttgcca
     2821 ggtgtcgtgc aggcggtacg ggctgtggtg ccttcgacat acgcggtcga cgcgttcgcc
     2881 gaatccttca aagcaacacc gagttggacg acgatcagtg ccgatctcgc gatctgtgtc
     2941 ggtgtgtgcg tgctggcgtt gaccgcctcg gcgtgggcgt ttcgccgtgc cgtccgctcc
     3001 cgttgaaccc cgccggttac ggtgagcacg tgagcgacga ctcgacagat ccgcgatcga
     3061 atgggtcggc ccccgagccg tcggaaccaa cgctcgccta tccgcagccg cggcccatcc
     3121 cgtacgggtc gtcgccgtac gcggtgaccg tcaccaccac caacccggtt cctgacgtcc
     3181 gccagcagcc gctgctcgaa ctggccgatc tgtggactgt gattctcggc actatctgcg
     3241 tgatcccgct tggagtcgtc gcgggcttca gctggctgtg gcttgcgcca cgcgcgatgg
     3301 ccgtcgctga cggcaagggc aagaccggcc tcctggaccc cggcaccaag gccttcgccg
     3361 gcgccgacgt gacgttcctc ttcatcacga tcgtggccgg gctgctgtgc ggagtcgtgg
     3421 cggcgatcat cgcgaggcac cgcggggtgg cggtcagcgt ggcgctcgcc gccggcggta
     3481 ccaccgcggc ggttctcgcc gcgtggatcg gccgcacgat gtcgggcggc ccagcgaagc
     3541 actgggcaag ccacgtcgcc gcaggcaacc accgctactt tctcgaactg gcaacccggc
     3601 aatacctcgt cgcctggccg ctggccgcgc tggcggtcac gttcgtcgtc gcgctgttga
     3661 cgcccgaccg cccggtcgag atcgtgccga cggagccagc gccagcggag tcgggaccga
     3721 gctagtaccg gctagtcccg ggcgatcgcc gcgagcgtgg cgcctgtcat cgcgacgagg
     3781 tcgcgggcag cgagttcgac gtcgagcccg cggcgtccgg ctgagacata aatcgtcgga
     3841 tggtcaagcg ccgacgcgtc gatcaccgtc ggaagcttgc gccgctgccc tagggggctg
     3901 atgccgccga cgacgtaccc cgtcgtgcgt tcggcgaccg cgacgtcggc catcgcggca
     3961 cgtttgccac ccgtcgcctc ggcgagcgcc ttcaggtcaa gtgcgcccga gaccgggact
     4021 accgcgacga ccagttggcc gtcgacgtcg accagcaagg tcttgaagat tcgggacgtc
     4081 gacaccccga gcgccttcgc tgcctcgtcg ccgaaggacc tgatgctcgg gtcatggtcg
     4141 taggggtgca cggtgtggcc gacgccggcg cgcgcaagga cgacgagtgc cggggtgccg
     4201 ttggtcacgg cgggggagtc tagaaccgct gatgatcacc ccgccactgg gccgcaggtg
     4261 tcctgccgcg tgcgtacccc gccgcctgct agtctgtacc ccgacgacgg cacaacatgc
     4321 cgtccctccc gcgggcgagc cgcccgcgag gctacgtccc tcccaggatc agaacctctt
     4381 catcagaaac tctgatcctt tggcgcgcaa gcagcgctcc acgtcgaacc tgacgatgat
     4441 gacgatgcct ccgcaatacc gacggagcgt caccttttat gtctgcccct tcttactcgt
     4501 ccacgtctgc gcgcgcctca ggtgcgcctt tcagcgcccg ctcgtcgggc gccccctccc
     4561 gcggcaccgc ccctaatcgg ggcgccgcac ccagccgcag cacaacccgc cggcccaacc
     4621 gtggcgccag caagtcgacc cagtcgcgtg catcccagtc gccgaaggcg tcggctgcct
     4681 ccgaactcga gcgcttgctc gacctcgcga tggcgcagcc cgcacccgcc gaccgcacct
     4741 tcgccgaact cggcctcccc aaggctctcg tcatggcgct gagcaagcgg ggcatcgagt
     4801 cgccgttcgc gatccagtcg cacgcgattc ctgatgctct cgcgggccgt gacgttctcg
     4861 gccgcgccca gaccggtggc ggcaagacga tggcgttcgg gcttccattg ctcgcccgac
     4921 tcgcgacctc gggctcgatg aggatgtcga aggcaccacg agcgctggtt ctcgtcccga
     4981 cccgcgaact cgcgatgcag gtggctgacg cgcttcgccc gctcggcgag gcgctcgggc
     5041 tcaagctgtg ctgcgttttc ggcggtgcgc cgtatggcaa gcagatcgac gcgctgaacc
     5101 gtggcgtcga cctcgtggtc gcgacacctg gccggttgat cgacctcctc gagcgccgtt
     5161 gcctggtgct ctcagccgtt gaggtagccg tgatcgatga ggccgaccac atggccgacc
     5221 tcggcttcct gccggcggtc accacgatcc tcgaccagac cgcgccagac gggcagcgga
     5281 tgctcttctc cgcgaccctc gaccgtggtg tcgacaagct cgtgcgtgcc tacctcaagg
     5341 atcctg
//
LOCUS       DASWVF010000502         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_344733, whole genome shotgun sequence.
ACCESSION   DASWVF010000502 DASWVF010000000
VERSION     DASWVF010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344733"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /gene="pknB"
                     /locus_tag="VGD55_08965"
     CDS             complement(<1..1363)
                     /gene="pknB"
                     /locus_tag="VGD55_08965"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY0870517.1"
                     /translation="MLDAVDTTTSDALVGRVLDGRYRVEARVARGGMATVYRALDTRL
                     DRVVALKVMHQIFAENDQFVARFIREAKSAARLSHPNVVAVYDQGEDAGYVFLAMEYV
                     QGRTLRDLLRERTRLAPTDALSILEPVLAALSAAHAAGLVHRDVKPENVLLADDGRVK
                     VADFGLARAAANLEATSATSLIGTVAYLAPEQVIRGIADPRSDVYAAGIMLFEMITGR
                     QPYDGETAISVALRHAHEDVPAPSSFVSGVPESVDLLVARATARDADRRPHDAGAFLA
                     EVVRVRSGLVAAGANAVSLQELTQNTLVVELPDTMSVGADPPWQSGRASAPPPAVTPP
                     PPDDEDEYYRPHPHRRGWIAFWVILLLAAAAVAAGWWFAKGRWTTTPNLASLTSSAAI
                     TKAKAAHVTVKVGDPIVSDKVPANFVAEQTPAASHKVLRGDAVTIHLSSGPQLYQVPS
                     FNAG"
     gene            1476..2132
                     /locus_tag="VGD55_08970"
     CDS             1476..2132
                     /locus_tag="VGD55_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0870518.1"
                     /translation="MHPRVVLFDLDGTLTASGPGITRSAASALQAVGRSPLDEAALLG
                     FIGPPLIESFREIAGLDEAAVIEALVAYRSYFVERGMFENSVYPGIMTLLQELCDAGR
                     RLAVTTSKPLPYAVPIIEHFALGGYFEGVFGPTADDDGIDKKAVVAHALNAMAIDDTT
                     KVVLVGDRFHDVVGAHENGIACIGALWGYGAREELLAAGVDALAADADELAILLGLRA
                     "
BASE COUNT          374 a          716 c          794 g          388 t
ORIGIN      
        1 ctccagcatt gaacgatggc acctgataca gctggggccc gctcgacaga tggatcgtga
       61 cggcgtcacc gcgtaacacc ttgtgagacg ccgctggtgt ctgctcagcg acgaagttcg
      121 cgggaacctt gtcggagacg atcgggtcgc cgaccttcac cgtgacgtga gcggctttcg
      181 ccttcgtgat ggcagccgac gacgtcaggc tggccaggtt gggcgtggtc gtccagcggc
      241 ccttcgcgaa ccaccagccg gcagcgacag cggcagctgc taggagcaag attacccaga
      301 atgcgatcca gccacgtcgg tgcggatgcg ggcggtagta ctcgtcttcg tcatcgggcg
      361 gtggcggcgt gacggcaggc ggcggcgcgc tggcgcggcc tgactgccag ggcggatcgg
      421 ctccaaccga catcgtgtcg ggaagctcga cgacaagcgt gttctgggtc aactcctgca
      481 agctcaccgc gtttgctcct gcagcgacaa ggccgctgcg cacgcgcacc acctcagcga
      541 ggaacgcacc ggcgtcgtgc ggtcggcgat ccgcatcgcg tgcagtggcg cgggcgacga
      601 gcaggtcgac cgattcaggg acgccggaga cgaaggacga cggcgccggt acgtcctcgt
      661 gcgcgtggcg caacgccacc gagatcgcgg tctcgccgtc gtagggctgc cggcccgtga
      721 tcatctcgaa caacatgatg ccggcggcgt agacgtcgct tctcgggtcg gcgatgccgc
      781 ggatcacctg ctcgggcgcg aggtaggcga cggtgccgat cagcgacgtc gcgctggtcg
      841 cctcgaggtt ggcggccgcc cgcgccagcc cgaagtcggc caccttgacg cggccgtcat
      901 cggcaagcag cacgttttcg ggcttcacgt cacggtgcac aaggccggcg gcgtgagcgg
      961 ccgagagcgc cgcgagcacc ggctcgagga tcgagagcgc atcggtcggc gccagccggg
     1021 tgcgctcccg caacaggtcg cgcagcgtgc ggccctggac gtattccatc gccaggaaca
     1081 cgtaacccgc gtcttcgccc tggtcgtaca ccgcgacgac gttggggtgc gacagccggg
     1141 cggccgactt cgcctcgcgg atgaatcggg ccacgaattg atcgttttcg gcgaagatct
     1201 gatgcatcac cttgagcgcg acgacgcggt caaggcgggt gtcgagggct cggtagacgg
     1261 tggccatgcc gccgcgtgcg acgcgcgcct caacccggta acggccatcg agcacgcggc
     1321 cgactagggc gtccgacgtc gtcgtatcca cggcgtcgag catacgtgcg gagccctccg
     1381 gtacagggca ggcgcgacgc tttggcacgg gacgtcgaag gggctggagg tcgggtgacg
     1441 gccggcggtt gaacgggata cgggaggatg gccaggtgca tccacgcgtg gttctcttcg
     1501 atcttgacgg cacattgacg gcgtcgggtc ccggcatcac ccgctccgcg gcgtccgccc
     1561 tgcaagctgt gggccgctcg ccgctcgacg aggcagccct gctgggcttc atcggcccac
     1621 cgctgatcga gtcgtttcgc gagatcgccg ggctcgacga ggccgcggtg attgaggcgc
     1681 tcgtcgccta tcggagctat ttcgtcgaac gcggcatgtt cgagaactcc gtctaccccg
     1741 ggatcatgac gctgctgcaa gagctttgtg atgcgggacg ccgacttgct gtcactacct
     1801 caaagccgct gccgtacgcc gtgccgatca tcgagcactt cgcgctcggc ggttacttcg
     1861 agggtgtgtt cgggccaacg gctgacgacg acggcatcga caagaaggcc gtcgttgcgc
     1921 atgcgctgaa cgcgatggcg atcgacgaca cgacaaaagt tgtgctcgtc ggtgatcggt
     1981 tccacgatgt cgtcggagcc cacgagaacg gcatcgcatg catcggtgcg ttatggggtt
     2041 acggtgcgcg agaggaactt ctcgccgcag gtgtcgatgc actagcagcg gacgccgacg
     2101 agctggcgat cctgctcggc ctgcgagcct gatgtgcagt ttgctgtcca cgcgcgaact
     2161 ggatgttcga tggcaaagaa ttcattcgct cacgcgcaca tctgccgatt tgcggtcgta
     2221 tatcgcggcg ctccactggc tgggagactg aggtcatggg accggaacct gt
//
LOCUS       DASWVF010000503         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_318961, whole genome shotgun sequence.
ACCESSION   DASWVF010000503 DASWVF010000000
VERSION     DASWVF010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318961"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            655..>1690
                     /locus_tag="VGD55_08975"
     CDS             655..>1690
                     /locus_tag="VGD55_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870519.1"
                     /translation="MPRLVLLVGSLAVLGVLCVLGAAMEIAGAEPTPALWRWALVVAL
                     PIVGEVAKVGIRRGASRHGYSWRETAFVLGLVLVPPAHLVLAMTVGITVVALARRASM
                     PKVVFNAAVVAIETVVGCVIVESAHVYSSGLMWGAVFSMRTVVLIALATLAVHVVSTG
                     LTSAAVATERRRPVWLSVRDDLRVAVVIWVRNVGAALLIMASLSWSVWFTVLILCALL
                     GVQALSTDRAAIREERFAWHRLQVAIDELRDVELDQLIREATTAVATMMRADAAEIQL
                     DEATGHGTTVRASDIEDAHAPSQDEHRHGHSMDIELTTREGRIGELRLLFAGPVTLNA
                     TETDCLNAFAN"
BASE COUNT          272 a          529 c          556 g          333 t
ORIGIN      
        1 tcgcgagtcg cccccgcgtt ctcggtcgtg accaccaccg tgacggtcag tcgcacgcgt
       61 cccccagtct gcttcgccca cgctccattc ggacagtctc ggcgcgaatc cgcctacgtg
      121 ggggcattcc ggctgtgcct gtggacgacg gacctttggc acgctcagtg actcttcctg
      181 atcacccgcg gtgattactc cgaacggccc agtgaccaat tacgccattt gggtgtacca
      241 agagtgacgt atgcgtcagg atcggcaatg ccggacgtcg ggtgccagct ggcttttcgt
      301 ggatggacag cctgaaagag acccgaccgc ccggcgaacg tcgcgtgacg gtacctgcag
      361 ttgcgctcga gacccccggc gcgaaggagg tgcccgcatg cgcggatcga cctggttgta
      421 agcacggcca cgccgtttga ggttccacaa ccgcggcgtg agtaccagag aaggggccgg
      481 ttggcgccgg ccctttctca tgtccggaac ccggacatga gttccatcgg agtagcgaac
      541 gcccgagtaa tcggccgttc ggcctatgtg atccgccgtg ccgttgtcga tgttcctcta
      601 ggggatgttt cggccaaagg ggcggcgatg gttcagcgtg cgcggctgcg tgcgatgccg
      661 cgccttgtgc tgctcgtcgg cagcctcgcc gttctcggcg tgctttgcgt tctcggcgcc
      721 gccatggaaa tcgctggagc cgaacccacc cctgcccttt ggcgatgggc tctagtagtc
      781 gcgctcccca tcgtcggtga agttgccaag gtcggcatcc ggaggggtgc gagccggcac
      841 ggatacagct ggcgagagac ggcgttcgtg ctggggctcg tacttgttcc gccggcgcat
      901 ctcgtgctgg cgatgacggt gggtatcacc gtggtggccc tggcgcgacg agcgtcgatg
      961 cccaaagtcg ttttcaacgc agctgtcgtc gccatcgaga cggtcgtcgg ctgcgtgatt
     1021 gtcgagtcgg cgcacgtcta ttcgtccgga cttatgtggg gcgcggtgtt cagcatgcgc
     1081 accgtggtcc tcatcgcgct cgcgaccctt gcggtccatg tcgtctcgac cggcctcaca
     1141 tcggcggcgg tcgcgaccga acgtcgccga ccagtgtggc tcagcgtgcg cgacgacctg
     1201 cgcgtagcgg tggtcatctg ggtgcgcaac gtcggagctg cgcttctcat catggcgtcc
     1261 ctcagttggt cggtgtggtt caccgtgctg attttgtgcg cactcctcgg cgtccaggct
     1321 ctcagcacag atcgggccgc gatccgggaa gagcgattcg cttggcaccg gctacaggtc
     1381 gcaatcgacg agttgcgcga tgtggaactt gaccaactga tacgcgaggc gacgaccgcg
     1441 gtcgccacga tgatgcgcgc ggacgcggca gagattcagc tcgacgaagc caccgggcac
     1501 ggaaccacgg tgcgcgccag cgatatcgaa gatgcgcacg caccctcgca ggatgaacac
     1561 cgccatggtc acagcatgga tatcgagctg accactcgag agggccgcat tggagaattg
     1621 cggctgcttt tcgcagggcc agtcaccctc aatgcgaccg agaccgattg tttgaacgca
     1681 ttcgcgaatg
//
LOCUS       DASWVF010000504         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_166237, whole genome shotgun sequence.
ACCESSION   DASWVF010000504 DASWVF010000000
VERSION     DASWVF010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166237"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..986)
                     /locus_tag="VGD55_08980"
     CDS             complement(144..986)
                     /locus_tag="VGD55_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HEY0870520.1"
                     /translation="MLRGSPVRVLDDRDLVVVRDLLDRDPVANVFVSSRVDGAGLDPW
                     RLGAEVWGYLAGGRVEALCYAGANLVPVAGSADACRVFAEKARRQGRRCSSIVGPADQ
                     VGMLWALLESAWGPPRDVRPQQPLLAISDPPKLAPDPLVRRVRPDELDILYPACVAMF
                     TEEVGVSPANGDGGSLYRARVGELIAQGRAFARIEDGRVVFKAEIGAATTEACQIQGV
                     WVEPALRGRGYANGGMAAVVTYAQASVAPVVSLYVNDYNAAARAAYARVGFAQVGSFM
                     SVLF"
     gene            complement(1036..2169)
                     /gene="ispG"
                     /locus_tag="VGD55_08985"
                     /gene_synonym="gcpE"
     CDS             complement(1036..2169)
                     /gene="ispG"
                     /locus_tag="VGD55_08985"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0870521.1"
                     /translation="MPLVSPLAPRRVSRQLNVGGVLVGGNAPVSVQSMCITLTADINA
                     TLQQIAELTASGCQIVRVAVPSQDDADALPIIAKKSQIPVIADIHFQPKYVFAAIEAG
                     CAAVRVNPGNIKKFDDQVKEIAHAARDHGTPIRIGVNAGSLDPRMLAKYGGKATPEAL
                     VASAKWEASLFEEHDFHDIKISVKHHDPLIMIEAYRQLAAETDYPLHLGVTEAGPLMQ
                     GTIKSAVAFGILLSEGIGDTIRVSLSAPPVEEVKVGIGILESLGLRQRKLEIVSCPSC
                     GRAQVDVYKLAEEVTAGLEGMTVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFV
                     RGEVVKTVPEAQIVETLIEEAMRLADEMVSDGVASGEPQVTVV"
     gene            complement(2245..>3497)
                     /locus_tag="VGD55_08990"
     CDS             complement(2245..>3497)
                     /locus_tag="VGD55_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0870522.1"
                     /translation="GPLPHLRHRLTRRTLNMMAIGVLAFVVALLVSVMIHEAGHFVTA
                     KLFGMKATQFFVGFGPTLWSRRRGETEYGVQAIPAGGFVKIIGMTPLEDVAPEDQPRA
                     FINKPGWQRFIVLVAGSTMHFVIALVLLLILLLGWPSNLHGAAQVGTVENCVSTTTDQ
                     SCPAGAPPAPALGKFKPDDVITAVNGQPVVHGSQQLTSLIGNAAPGPITFTVQRAGST
                     LNLVVTPVVVDGRSRVGIQVGDAKNATYTRVGVAGAVTGAFKMFGTFFVGSFKAFGIV
                     PHEIGQLISHPAAKREVSANGGGGQVTSVFGVARFAGQAFQADGFSGGVATVVEIVAS
                     VNIFVGIFNLFPFLPLDGGHVAILFYEKVRNAWRRRRHLIAAGPVDLNKLMPFTYGVL
                     AIIVAVSALILVADIANPVANPFG"
BASE COUNT          621 a         1193 c         1128 g          555 t
ORIGIN      
        1 gcaagatcac ggcccgatcg tatctggcgg gctttccgcg cccgcccgac cacacagtgg
       61 acgtttactg ccccctgagg gcagtaaacg tccacgctgt gcgcagacct gacctagaag
      121 cggagacccg accgagacct gacctagaag aggaccgaca tgaacgagcc gacctgggcg
      181 aagccgaccc tcgcgtaggc ggcgcgcgcg gcggcgttgt agtcgttcac gtacaggctg
      241 accaccggcg ccaccgaagc ctgcgcgtac gtcaccacgg ccgccatgcc gccgttggca
      301 tacccacgcc cgcgcaacgc aggctccacc cacacgcctt ggatctggca cgcctctgtc
      361 gtcgccgcgc cgatctcggc cttgaagacc acccggccgt cttcgatccg cgcgaacgcc
      421 cggccctggg cgatcagctc ccccacccgc gcccggtaga gcgagccgcc gtcgccattg
      481 gccggtgaga cgccgacctc ctcggtgaac atcgccacgc aggccggata caggatgtcg
      541 agctcgtcgg gtcgcacccg ccgaacgagc gggtcgggcg ccagtttcgg ggggtcgcta
      601 atggcaagca agggctgttg gggccggacg tcgcgtggtg ggccccatgc ggattcgagc
      661 agcgcccaca acatgccgac ctgatctgcg gggccgacga tcgatgaaca tcgacgacct
      721 tggcggcggg ccttctcggc aaacaccctg cacgcgtcgg cgctgcccgc caccggcacc
      781 aggttggccc cggcgtagca gagcgcctcg acccggccgc cggcgagata cccccagacc
      841 tcggcgccga gccgccacgg atcaaggcct gcaccgtcga cacgggagct gacaaacacg
      901 ttcgcgacgg ggtcgcgatc gagcaagtcc cgcaccacga cgaggtcacg atcgtcgaga
      961 acccgcacgg gcgaaccacg cagcacgaac tgccctcgct tcggttccgg ggccgtcgcc
     1021 tgaggtcggg gctgcctaga cgacggtgac ctgaggctcg ccggaggcta cgccgtccga
     1081 caccatctcg tcagccaacc gcattgcttc ttcgataaga gtctcgacta tctgcgcctc
     1141 cggcacggtc ttgacgacct cgccgcgcac aaagatctgc ccttttccgt tgcccgaggc
     1201 gacgccgaga tcggcctcac gtgcctcgcc cgggccgttg acgacacacc ccatcacggc
     1261 cacccgcaac ggaaccgtca tgccttcaag gcccgcggtg acctcctcgg ccagcttgta
     1321 gacgtcgacc tgggcccgcc cgcacgacgg gcacgagacg atctcgagct tgcgctggcg
     1381 caggccgagc gactccaaga tcccgatgcc gaccttgacc tcttcgacgg gcggcgccga
     1441 gagcgagacg cggatcgtgt cgccgatgcc ctccgacagc aagatgccga aggcgacggc
     1501 cgacttgatc gtgccctgca tgagagggcc ggcctcggtc acgccgaggt gcaacgggta
     1561 gtcagtctcg gcggcgagct ggcggtaggc ctcgatcatg atcaacgggt cgtggtgctt
     1621 gaccgagatc ttgatgtcgt ggaagtcgtg ctcctcgaac aggctcgcct cccacttcgc
     1681 cgaggccacg agagcctcgg gtgtcgcctt gccgccgtac ttcgcgagca tccgcgggtc
     1741 gagtgagccg gcgttgacgc cgatgcggat cggcgtgccg tggtcgcggg ccgcgtgcgc
     1801 gatctccttg acctggtcgt cgaacttctt gatgttgccc gggttcacgc gcaccgccgc
     1861 acagccggcc tcgatcgcgg cgaacacgta cttgggctgg aagtggatgt cggcgatcac
     1921 cgggatctgg gatttcttgg cgatgatcgg cagcgcatcg gcgtcgtctt ggctcggcac
     1981 ggcgactcga acgatctgac atccggacgc cgtcagctcg gcgatctgct ggagcgtcgc
     2041 gttgatgtcg gcagtcaaag tgatgcacat cgactgcacc gagactggcg cgttaccgcc
     2101 gaccagcacg ccgccgacgt tcaactgccg cgacacgcgc cgaggtgcga gcgggctcac
     2161 gagaggcatc tccgggagtc ccaggttgac ggttgtcacg gtcttttcga gcctctcagt
     2221 cggactgcct cgagatcgac attactagcc gaacgggttc gccaccggat tcgcgatatc
     2281 ggccaccagg atcaacgccg acacggccac aattatggcc agaacgccgt aggtaaacgg
     2341 catcagcttg ttgagatcga ccgggcctgc cgcaatcagg tgtcggcgtc ggcgccaggc
     2401 gttgcgaacc ttctcgtaga agaggatcgc gacatggccg ccgtcgagcg gcaggaacgg
     2461 aaagaggttg aagatcccga cgaagatatt gaccgacgca acgatctcga ccacggttgc
     2521 gacgccaccc gagaacccgt cggcctggaa cgcctggccc gcaaatctgg cgactccgaa
     2581 aacgctggtc acctgccctc ctccgccgtt tgcgctcacc tctcgcttcg cggctgggtg
     2641 actgatcagc tggccgatct cgtgcggcac gatgccaaag gctttaaagc tgccgacgaa
     2701 gaaagtcccg aacatcttga aggcgccggt gacagcaccg gcgacgccga cccgcgtata
     2761 cgtggcgttc ttggcgtcgc cgacttggat gccgacccgc gatcggccgt cgacgacgac
     2821 cggtgtgacg acgaggttga gcgtgctacc ggctcgttgc accgtgaacg tgatcgggcc
     2881 gggcgccgcg ttgccgatca gcgacgtgag ctgctggctg ccatgtacga caggctgccc
     2941 gttgacggcg gtaatgacgt cgtcgggctt gaacttgccc agcgcaggcg ccgggggcgc
     3001 gcccgctggg cacgactggt cggtagtcgt gctcacacaa ttctcgacgg tcccgacttg
     3061 cgcggcgccg tggaggttcg acggccagcc gagcagcagg atcaacagca gcacgagggc
     3121 gatcacgaag tgcatcgtcg agcccgccac caacacgatg aaccgctgcc agcccggctt
     3181 gttgatgaac gcgcgcggct gatcctcggg cgcgacgtcc tccaggggcg tcatgccgat
     3241 gatcttgacg aagccgcccg ccggaattgc ctggactccg tactcggtct cgccgcgccg
     3301 gcgtgaccac agcgtcgggc cgaagccgac gaagaactgg gtcgccttca tcccgaaaag
     3361 ctttgcggtc acgaagtgac cggcctcgtg gatcatgacc gaaaccagca aggcgaccac
     3421 gaaggccagc acgccaatcg ccatcatgtt gagagttctc ctggtgagtc ggtgccgtag
     3481 atgagggagc gggcccg
//
LOCUS       DASWVF010000505         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_38959, whole genome shotgun sequence.
ACCESSION   DASWVF010000505 DASWVF010000000
VERSION     DASWVF010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38959"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..470
                     /locus_tag="VGD55_08995"
     CDS             39..470
                     /locus_tag="VGD55_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0870523.1"
                     /translation="MSAWLVWTIVAAACAAAEAVTLTLVLGFVSLAAVVALVVAVVGG
                     PVPLQLGAFIAGSIGLVGVARPIAKSHLRTPAELKSGIDALVGKSALVLERVDAHHGQ
                     VKIGGEIWTARAYMEDQVLEPGTSVDVVKIQGATALVYGTE"
     gene            474..1475
                     /locus_tag="VGD55_09000"
     CDS             474..1475
                     /locus_tag="VGD55_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0870524.1"
                     /translation="MSPELIALIVVAIFVLLVVGRTIRIVPQARAGIVERFGRYNKTL
                     TPGLNIVVPFVDRLRPLLDLREQVISFPPQPVITEDNLVVGIDTVLYFQVTDARAATY
                     EIANYIQAIEQLTVTTLRNVAGGMDLEKTLTSREEINSQLRVVLDEATGKWGIKVNRV
                     ELKSIDPPASIKDSMEKQMRADRDKRAAILTAEGFKQSQILTAEGEKQSAILKAEGQA
                     QAAVTQATADAKAQALRAEGQAKAIGTVFQAIHEGKPDAELLAYQYLQMLPQIAQGTA
                     NKLWIVPSEMGKALEGLGSLVGGLTNGGSSSPNGAAATAPAKPAAIAAPPPPDDSAS"
     gene            complement(1468..>1652)
                     /locus_tag="VGD55_09005"
     CDS             complement(1468..>1652)
                     /locus_tag="VGD55_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870525.1"
                     /translation="GDHDGKADIMDPADAIYTAAAYLCANGAGQGGPSLTTAIWNYNH
                     ADWYVQLVESLASKIS"
BASE COUNT          299 a          527 c          527 g          299 t
ORIGIN      
        1 cgcccgcgcg gtgtgaagta ccggcctatg atcgagttat gtccgcttgg cttgtttgga
       61 cgatcgtggc agctgcctgt gccgccgccg aagccgtgac cctgacgctg gtcctcggat
      121 tcgtctccct tgccgcggtc gttgcgctcg tcgtcgcagt ggtcggtggt cccgtgccgc
      181 tgcaactggg tgccttcatc gccggctcta tcggactcgt tggggtggcc aggccgatcg
      241 ccaagtcgca cctgcgcaca ccggccgaac tgaagagcgg catcgacgcg ctcgtgggca
      301 agagtgcgct ggtcctcgaa cgggtagacg cccatcacgg tcaggtcaag atcggtggcg
      361 agatctggac cgcccgcgcc tacatggaag accaggttct cgaacctggg acgtcggtcg
      421 acgtcgtaaa gatccagggc gccaccgctc tggtctatgg aacggagtag ttcatgagtc
      481 ccgagctcat tgcgctcatc gtggtcgcga tcttcgtgct gctcgtcgtc gggcgcacga
      541 tccgaatcgt gccgcaggcc cgggcaggca tcgttgagcg tttcggtcgc tataacaaga
      601 cgttgacccc gggcctgaac atcgtggtgc cgttcgtcga ccgcttgcgc ccgctgctcg
      661 acctgcgcga gcaggtcatt tcgttcccgc cacagccggt catcaccgaa gacaacctgg
      721 tcgtcggcat cgacactgtc ctgtacttcc aagtcaccga tgcgcgcgcg gcgacgtatg
      781 agatcgcgaa ctacatccag gcgatcgaac aactcaccgt cacgacgctt cgtaacgtcg
      841 ctggtgggat ggatctcgaa aagaccttga cctcacgcga agagatcaac tctcagttgc
      901 gcgtcgtact cgacgaggcc accggcaagt ggggtatcaa ggtcaaccgg gtcgaactca
      961 agtcgatcga cccgccggcg tccatcaaag actcgatgga aaagcagatg cgggccgacc
     1021 gtgacaagcg cgccgcgatc ctcaccgccg agggcttcaa gcagtctcaa attctcacgg
     1081 cagagggcga aaagcagtcg gccatcttga aggccgaggg tcaggcccag gctgcggtca
     1141 ctcaggcgac ggctgacgca aaggcccaag cgcttcgggc agaaggccaa gccaaggcga
     1201 tcggcaccgt ctttcaagcc atccacgagg gcaagccgga cgccgagttg ctggcgtacc
     1261 aatacctgca gatgcttccg cagatcgccc aaggtacggc gaacaagttg tggatcgtcc
     1321 cgagcgagat ggggaaggcg ctcgagggcc tcggcagcct tgtcggcggc ctcaccaacg
     1381 gtgggtcaag ttcgccgaac ggcgccgcgg caacggcgcc ggccaagccg gccgcgatcg
     1441 ccgcgccacc accgcccgac gactcagcta gctgattttg ctggctagcg actccacgag
     1501 ctgcacgtac cagtcggcgt ggttgtagtt ccagatcgcg gttgtcagtg atggtccgcc
     1561 ttgcccggcc ccgttcgcgc agaggtacgc ggccgcggtg tagatggcgt cagccgggtc
     1621 catgatgtcg gccttgccat cgtggtcgcc gt
//
LOCUS       DASWVF010000506         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_166280, whole genome shotgun sequence.
ACCESSION   DASWVF010000506 DASWVF010000000
VERSION     DASWVF010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166280"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /gene="cynS"
                     /locus_tag="VGD55_09010"
     CDS             <1..369
                     /gene="cynS"
                     /locus_tag="VGD55_09010"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:TIGR00673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEY0870526.1"
                     /translation="DACGRSEVWTTSALLGQQAMSASEAAAVAPLLDLGPDDVTLLQV
                     PPSKGSLTATPPTDPLIYRFHEIVQVYGTTLKELIHENFGDGIMSAIDFEMFVDKLPD
                     PKGDRVKVTMHGKFLPYRKW"
     gene            complement(377..3262)
                     /locus_tag="VGD55_09015"
     CDS             complement(377..3262)
                     /locus_tag="VGD55_09015"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEY0870527.1"
                     /translation="MSSRSQTQATGALLLMFAAMLAAPARADQDDDDRPRDGRTLLAT
                     GQFVTPLAPPDAQQQFLNPGLANYPNFVANEAVRSQLSPDGTTLLILCAGYNTNLDKN
                     AQVDLPASSQYIFVFDVAGARRSRPKLRQVIHQKNAYVGLVWAPSGQTFYATGGNDDA
                     VYAYRQSGGKWAQSAKIALGHSVGAGLNLPGVFTTEPNAAGLAISADGSTLVVANNYN
                     DSISVIDTATASVRYEHDLRPYFPNNEGTDGVAGGEFPHAVVIKGNGTAYVSANRDRE
                     VVVVDISALNAGRLIKRIALDGLPNGIALNAAQTKLYVAQDNADQVAVIDTASNAVTA
                     KIDTRAPLGMLAPGYTGAAPFAVTISPDQKTLFAVNDGANSIAVIPLAGPHANTVTGL
                     IPTAYGPKDITLSADGRWMYIINGKSEQGPNPLNLFYDTDKLTTVTYPGGNAAAAVAA
                     AAANQYQLNNDRSTLVSARVPSGEDLEELTRQVALNNFYSVPPNEHDERVMKFLRSKI
                     HHVIYIVKENRTFDQVLGDLQNGSQGDASLAVFGKQITPNFHRIASHFVTLDHFLDTG
                     DASMDGWGWSLQGRATSTLSLTQNINYAGVDRGLSYLSEGTNREVPTGLTVPQRLAIF
                     GSIYSSALAGQPGGANNVLPGTADVAASDAPFGKGKGYIFDAVLAAGGTLRNYGFLTY
                     NVGPLVDNPGAVGAQQVVPLQKTMVQPGITDLFFRGFDQDYPDLWRYHEWKREFDQYV
                     ANGNLPTMQTVRFNHDHTGSYSSAVAGVNTPETQQADNDLSTGKLIEDIAHSRYARDT
                     LIFIIEDDSQDGPDHIDSHRSTAYVVGPYVKKNDVIGKHYSTVSMLRTIEDVLGLQHL
                     NLNTAYQRPMTEVFDIHCDGSWDYRAVASKILQTTTLDLSGEQFAEGPAVKPTHTAVW
                     WARQTRGFDWSQEDRVPVDRYNRIIWRGMKGNAPYPLFAAKGTKRGD"
BASE COUNT          514 a         1039 c         1135 g          633 t
ORIGIN      
        1 gatgcgtgcg gacgcagcga ggtgtggaca acgtcagccc tactcgggca gcaggcgatg
       61 agcgcctccg aagccgcggc tgttgccccg ctcctggacc tcggccccga cgacgtcacc
      121 ttgctgcagg tgccgccctc aaaagggtcg ctcacggcga cgccgccgac cgaccccctc
      181 atctatcggt tccacgagat cgtgcaggtg tatggcacga ccctgaagga gctgattcac
      241 gagaatttcg gtgacggcat catgagcgcc atcgacttcg agatgttcgt cgacaagctc
      301 cccgacccga agggcgaccg cgtgaaggtg acgatgcacg gcaagttcct gccttaccgc
      361 aagtggtaag gcagcttcag tcgccgcgct tggtgccttt ggctgcgaac agcgggtacg
      421 gagcgttgcc cttcatcccg cgccagatga ttcggttgta gcggtcgacc ggcacgcgat
      481 cctcctgcga ccagtcgaag ccgcgggtct gccgcgccca ccaaacggcg gtgtgagtcg
      541 gcttgacggc cgggccctcg gcgaactgct caccgctcag atcgagcgtc gtggtttgca
      601 ggattttcga cgccaccgcc cggtaatccc agctcccgtc gcagtggatg tcgaacacct
      661 cggtcatcgg tcgctggtag gcggtgttga ggttcaggtg ctgcaggccg agcacgtcct
      721 cgatggtgcg caacatgctg acggtgctgt agtgcttgcc gatgacgtcg ttcttcttca
      781 cgtacggacc caccacgtag gccgtcgagc ggtgcgagtc gatgtggtcc ggcccgtcct
      841 gcgaatcgtc ctcgatgatg aagatcagcg tgtcgcgcgc gtagcggctg tgggcgatgt
      901 cctcgatcag cttgccggta gaaagatcgt tgtcggcctg ctgcgtctcc ggcgtgttca
      961 ctcccgccac cgccgaactg tagctgccgg tgtggtcatg gttgaagcgg accgtctgca
     1021 tggtcgggag gttgccgttc gcaacatact gatcgaactc gcgcttccac tcgtggtagc
     1081 gccacagatc ggggtagtcc tggtcgaagc cgcggaagaa caggtctgtg atgcccggct
     1141 gcaccatcgt cttctgcaac ggcaccacct gctgcgcacc cactgcgccc gggttgtcga
     1201 ccagggggcc gacgttgtag gtcaagaagc cgtagttgcg cagcgttccg ccggccgcca
     1261 gcaccgcgtc gaagatgtag cccttcccct tgccgaacgg cgcatcgctg gcggcaacgt
     1321 cggcggtccc cggcagcacg ttgttggccc cgcccggctg gcccgccagc gccgaggagt
     1381 agatggaccc gaagatcgcg agccgttgcg gaacggtgag gcccgtcgga acctcgcggt
     1441 tggtgccctc ggatagatac gagaggccgc ggtcgacgcc ggcgtagttg atgttctggg
     1501 tcagcgacag cgtgctcgtc gcgcgcccct gcaacgacca gccccagccg tccatgctgg
     1561 cgtcgccggt atccagaaag tgatccagcg tcacgaagtg gctcgcgatt cgatggaagt
     1621 tcggcgtgat ttgcttgccg aacaccgcca gcgacgcgtc gccctgggag ccgttctgga
     1681 gatcgccgag gacctggtcg aaggtccggt tctctttgac gatgtagatc acgtggtgga
     1741 ttttcgagcg cagaaacttc atcacccgct cgtcgtgctc gttgggcggg accgaataga
     1801 agttgttgag cgcgacttgc cgggtcagct cctcgaggtc ttcgccgctc ggaacccgcg
     1861 cactgaccag cgtcgagcgg tcattgttga gctggtactg attggccgcg gcggccgcga
     1921 ccgcggcggc cgcgttccca cctgggtacg tcacggtcgt cagcttgtcg gtgtcgtaga
     1981 acaggttgag ggggttgggc ccctgctcgc tcttgccgtt gatgatgtac atccagcggc
     2041 catcggcgct gagcgtgatg tccttcggcc catacgccgt cgggatgagt ccagtgaccg
     2101 tgttggcgtg cggaccggcc agcgggatca cggcgatcga gttcgcgccg tcgttgaccg
     2161 cgaagagggt cttctggtcc ggagagatcg tcaccgcgaa aggcgccgcg ccggtgtagc
     2221 ccggcgccag cattcccagc ggggcacgcg tgtcgatctt ggccgtgacc gcgtttgacg
     2281 cggtgtcgat caccgccacc tggtcggcgt tgtcctgcgc gacatacagc ttcgtctgcg
     2341 cggcgttcag cgcgatgccg ttcggcagac cgtcgagagc gatgcgcttg atcagcctgc
     2401 cggcgttgag tgccgagatg tcgacgacga cgacctcgcg atcgcggttg gcggacacgt
     2461 acgcggtgcc gttgcccttg atgacgacgg cgtgggggaa ctcgccgccg gcgacgccgt
     2521 cggtgccctc attgttcggg aagtatgggc gcaggtcgtg ctcgtaacgg accgaagcgg
     2581 tggccgtatc gatgacgctg atcgagtcgt tgtagttgtt ggcgacgacc agcgtgcttc
     2641 catcggcgga gatcgccagg ccggccgcgt tcggctcggt ggtgaagacg ccgggaaggt
     2701 tcaggcccgc gcccaccgag tggcccagcg cgatctttgc cgactgtgcc cactttccgc
     2761 cgctttggcg gtaggcgtag acggcgtcgt cgtttccccc ggtcgcgtag aaggtctggc
     2821 ccgacggcgc ccagacgagg ccgacgtagg cgttcttttg atggatgacc tggcgcagct
     2881 ttggcctcga ccgtcgagct ccggcgacgt cgaagacgaa gatgtactgg ctggaggccg
     2941 gcagatcgac ctgcgcgttc ttgtcgagat tggtgttgta gcccgcgcag aggatcagca
     3001 gcgtggtgcc gtccgggctc agctgcgagc gcaccgcttc gttggcgacg aagttcggat
     3061 agttggcgag gcccgggttc aggaactgct gctgcgcgtc cgggggcgcg agcggcgtga
     3121 cgaactggcc cgtggccaag agagtccggc cgtcgcgcgg ccggtcatcg tcatcctgat
     3181 ccgcgcgcgc cggcgcggcc agcatcgcgg cgaacataag gagcaacgct ccggtcgctt
     3241 gggtctgact cctcgagctc atctgggacc cctggatctc gcgagcgcag actcgacgtc
     3301 cgctcgcagg aatccagacg g
//
LOCUS       DASWVF010000507         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_141164, whole genome shotgun sequence.
ACCESSION   DASWVF010000507 DASWVF010000000
VERSION     DASWVF010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141164"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2324
                     /locus_tag="VGD55_09020"
     CDS             <1..>2324
                     /locus_tag="VGD55_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY0870528.1"
                     /translation="VTAANLPWAADSVVQDAGVNAAIDTLTTDGTNIYGGGYVFGAGG
                     NLEGTFEADPTTGAIKWLEDCHGDTYSTYSDGNVMYEVGHQHYCDDVPDAFPQNNPWV
                     IHRSQAFSTAVAGTLLHNTQSAYADFGGTPAPEQLDWYPTITSGTYTGQNQGAWTVTG
                     NGQYLVEGGEFPSVNGVAQQGLVRFAVRAIAPNKMGPQVTGQNFVPTITSITSGTARV
                     AFQANWDQDNTALTYKVVRNSNTASPVYTTTVNSTFFNRPMLGFTDTGLVVGTTYKYR
                     LYVTDPLGNQVAGDTVSFTPTANTVSPYAQAIINDAPNTYWRLGESTGATAYDWAGYS
                     DGIVGAGVTRGAAGAITGDPNTASTLNGTATAIISHPLSQIAPATFSIEGWFQTTSTT
                     GGRILGFGDTQTGTSGVADRTVYMTNSGQLEFGVISGSSVTGTKTTIASTGSYNDGQW
                     HYVVASLGSAGMSLYVDSKRVAQNAAVTTGIHYQGWWRLGGDTLSGYPSTPSNVNFTG
                     SVDDFAFYSTPLTAAQIANHYNASGRTAAGARPTDTYGSTIYDAAPNFYWRLDETSGT
                     VAHDVTPNGNNGNYTGGVTFGTPGAITGTTDTAVTLNGTNGLISTTAAVPGPTVYSEE
                     LWFKTNTTTGGKLIGFGSAATGSSGSYDRMVYMLNNGELTFGAYTGTTNTITSSASYN
                     DGKWHQMVATEASDGMKLYVDGALVASGTNTGSQSYNGYWRVGGDNIASWPNQPTSSY
                     FAGSVDEAAIYPNELSAAQVSDHFAKGNGAPNVP"
BASE COUNT          478 a          815 c          689 g          342 t
ORIGIN      
        1 ggtgaccgcc gcgaacctgc cctgggcagc cgactccgtc gttcaggacg ccggggtcaa
       61 cgccgcgatc gacaccctca ccactgacgg caccaacatc tacggcggcg gctacgtctt
      121 cggcgccggc ggcaacctgg aaggcacgtt cgaggccgac ccaacgactg gcgccatcaa
      181 gtggctcgag gactgccacg gtgacacgta ctccacctac tccgacggca acgtcatgta
      241 cgaagtcggc caccagcact actgcgacga cgtccctgac gcattcccgc agaacaaccc
      301 gtgggtaatc caccggtcgc aggcgttctc aaccgccgtt gccggcacgt tgctgcacaa
      361 cacccaaagc gcctacgccg acttcggtgg cacgccggca cctgagcagc tcgactggta
      421 cccgacgatt acttccggta cctacaccgg ccagaaccag ggcgcgtgga cagtcacggg
      481 caacgggcag tacctcgttg agggtggcga gttcccgagc gtcaacggcg tcgcacagca
      541 gggtttggtg cgcttcgcgg ttcgcgcaat cgccccgaac aagatgggtc cgcaggtgac
      601 cggtcagaac ttcgttccga ccatcacttc gatcaccagc ggcaccgcgc gcgttgcatt
      661 ccaggccaac tgggaccaag acaacactgc gctgacctac aaggtcgtgc gcaatagcaa
      721 caccgcaagc ccggtctaca cgaccacggt gaactcgacc ttcttcaacc gtccgatgct
      781 cggtttcacc gacaccggcc tcgtggtcgg gaccacgtac aagtaccggt tgtacgtcac
      841 agacccactg ggcaaccagg tcgctggcga cactgtctcc ttcacaccga ccgccaacac
      901 ggtgagccct tacgcgcagg cgatcatcaa cgacgcgccg aacacgtact ggcgcctggg
      961 tgagtcgacc ggtgccacgg cctacgactg ggccggttac tccgacggca tcgtcggtgc
     1021 cggcgtcacc cgcggagcag ctggcgcgat caccggcgac ccgaacacgg cctcgacgtt
     1081 gaacggcaca gcgaccgcga tcatctcgca cccgttgagc cagatcgccc cggccacttt
     1141 ctcgatcgaa ggttggttcc agacgaccag cacgacgggc ggccggatcc tcggcttcgg
     1201 cgacacccag acaggtacga gtggggttgc cgaccgaacc gtctacatga cgaacagtgg
     1261 ccagctcgag ttcggggtga tctccggctc cagcgtgacc ggcacgaaga ccacgatcgc
     1321 tagtaccggc tcatacaacg acggccagtg gcactacgtg gtcgctagcc tcggcagtgc
     1381 cggcatgtcg ctctacgtcg actccaagcg agtggctcag aacgccgcag tgaccacggg
     1441 catccactac caaggttggt ggcgcctggg cggtgacacc ctgagtggct acccgagcac
     1501 gccgtcgaac gtcaacttca ccggatccgt cgatgacttc gcgttctact cgacaccgtt
     1561 gacggcagcg cagatcgcca accactacaa cgcaagcggc cggacggcgg cgggcgcgcg
     1621 tccgaccgac acctacggtt cgaccatcta cgacgcggca ccgaacttct actggcggct
     1681 cgacgagacg tcaggcaccg ttgcgcacga cgtcacgcct aacggcaaca acggcaacta
     1741 caccggtggt gtcacgttcg gcacccctgg cgccattacg ggcacgaccg acactgcggt
     1801 caccctgaac ggcaccaacg gcctgatctc gacgaccgcg gcagtgcccg gaccgaccgt
     1861 ctacagcgaa gagttgtggt tcaagacgaa cacgacaacc ggtggcaagc tcatcggctt
     1921 cggtagcgca gctacgggta gcagcggcag ctatgaccgc atggtctaca tgctcaacaa
     1981 cggcgagctc acgttcggcg cctacacggg caccacgaac acgatcacca gctcggcgag
     2041 ctacaacgac ggcaagtggc accagatggt cgccaccgag gccagcgacg gcatgaagct
     2101 ctacgtcgac ggcgcgctcg ttgcgagcgg cacgaacact ggctcgcagt cgtacaacgg
     2161 gtactggcgg gtcggcggcg acaacatcgc gtcgtggccg aaccagccga ccagcagcta
     2221 cttcgccggc tccgtcgacg aggcagcgat ctacccgaac gaactctccg cagcgcaggt
     2281 cagcgaccac ttcgcgaagg gcaacggcgc gccgaacgtg ccgc
//
LOCUS       DASWVF010000508         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_421437, whole genome shotgun sequence.
ACCESSION   DASWVF010000508 DASWVF010000000
VERSION     DASWVF010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421437"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VGD55_09025"
     CDS             <1..1679
                     /locus_tag="VGD55_09025"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY0870529.1"
                     /translation="QVAQLSKFAGGIGISWSRVRSRGALIRGTNGHSNGIVPWLRTLD
                     SSVAAVNQGGRRKGAACVYLETWHPDIEEFLELRDNTGEDARRAHNLNLANWIPDEFM
                     RRVEADEMWSLIDPDQVPSLPDLWGDAFERVYRQAEVDGLVVRQVKARDLYSKMMRTL
                     AQTGNGWMTFKDASNRTSNQTALASNVVHLSNLCTEIVEVSNDGETAVCNLGSINLAA
                     HLLHDADGVAIGIDWPKLRATARTAVKFLDRVIDINYYPSAQAAASNPRWRPVGLGVM
                     GLQDVFFALRLPFDSARAMTLSTQLAEDIYVTSLEASCELAAESGPHPAYSETRAAQG
                     QLQPDLWGIEVLQVERWQALRVRIAEFGLRNSLLIAIAPTATIASIAGCYECIEPQVS
                     NVFKRETLSGEFLQVNAALVRELKVHGSWTPEVRNQIKRDDGSIAGVSSVPAEVRELF
                     RTAWELPQRSLIELAASRAPYVDQSQSLNLFLASPTIGKLSSMYLHAWKSGLKTTYYL
                     RSRPATRIQQATVAAIEVSAPPEPAAEIVSDDAAALACSLENPDSCEACQ"
BASE COUNT          322 a          544 c          575 g          318 t
ORIGIN      
        1 cgcaagtcgc acagttgtcg aaatttgctg gtggcattgg gatttcctgg tctcgcgtgc
       61 ggtcgcgcgg tgcgctgatc cgcggcacga acggccactc gaacgggatc gtgccgtggc
      121 tgcgaaccct cgactcctca gttgccgcag tgaatcaggg cggacgccgg aagggagccg
      181 cgtgtgtcta cctcgagact tggcacccag atatcgagga gttcctcgaa ctgcgcgaca
      241 acaccggtga ggatgctcga cgagcccaca acctgaacct ggcgaactgg attccggatg
      301 agttcatgcg ccgtgtcgag gccgacgaga tgtggtcgct gatcgacccc gaccaggtgc
      361 cgtcgctgcc agacctttgg ggcgacgctt ttgagcgggt ctaccggcag gccgaggtcg
      421 acgggctggt cgtcaggcaa gtcaaggcgc gcgacctcta cagcaagatg atgcgcacgc
      481 tcgcgcaaac tggcaacggc tggatgacat tcaaggacgc ctcgaaccgg acgtcgaacc
      541 agaccgcgct cgcgtcgaat gtcgtgcacc tgtcgaactt gtgcacggag atcgtcgagg
      601 tcagcaacga cggcgagacc gcggtgtgca acctcggttc gatcaacctc gcggcgcatc
      661 tgctgcacga cgccgatgga gtcgcgatcg gcatcgactg gccgaagctt cgagccacgg
      721 cccgcaccgc ggtgaagttc ctcgaccggg tcattgacat caactactac ccgagcgcgc
      781 aggcggcggc gagcaacccg aggtggcggc cggtgggtct cggcgttatg ggtctacaag
      841 acgtgttctt cgcgctgcgg ttgccgtttg actccgcgcg ggcaatgacg ctgtcgaccc
      901 aactggccga agacatctac gtcacatcgc tcgaggcgtc atgcgagctg gccgccgaat
      961 ccggtccgca tccggcgtac tccgagactc gcgctgcgca gggccagttg cagccggacc
     1021 tgtggggcat cgaggtcttg caggtcgaac gctggcaggc gttgcgggtg cgcatcgccg
     1081 agttcggtct gcgtaactcg ttgctcatcg cgattgcgcc gacggcgacg atcgcctcga
     1141 ttgccggctg ctacgagtgc atcgagccgc aggtgtcgaa cgtgttcaag cgcgagacgc
     1201 tgtcgggtga gttcctgcag gtcaatgccg cgctggtgcg cgaactcaag gtgcacggga
     1261 gttggacgcc agaggttcgt aaccagatca aacgcgatga cggttcgatc gccggcgtca
     1321 gctccgtacc agcggaggtt cgcgagcttt tccggacggc gtgggaattg ccgcagcgat
     1381 cgctcattga gctggctgcc agccgagctc cgtacgtcga ccagagccag tcgctgaact
     1441 tgttcctggc gtcgcccacg atcgggaagc tgtcatcgat gtacctccac gcctggaagt
     1501 cgggattgaa gacgacgtat tacttgcgat cgcggccggc aacccgcatt cagcaggcca
     1561 ccgtcgctgc gatagaggtg agcgcaccac cagagccggc tgcagagatt gtctccgacg
     1621 atgccgctgc cttggcctgc tctttggaaa accccgacag ctgcgaagcc tgtcagtaaa
     1681 ggagaatcga catgactatc gacgcgcggt acatcgacct cgacgctccg gcgtcgcagc
     1741 cggctcggct actcgaccc
//
LOCUS       DASWVF010000509         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_39055, whole genome shotgun sequence.
ACCESSION   DASWVF010000509 DASWVF010000000
VERSION     DASWVF010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39055"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..849)
                     /locus_tag="VGD55_09030"
     CDS             complement(154..849)
                     /locus_tag="VGD55_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0870530.1"
                     /translation="MNATATPPALIFDFDGLLVDTESTALRAWREQFAVHELDFPLDT
                     WHRYIGSQGSQEAMLAAIREGGVDFDDAELRADWRKRHNAIAAVEPLRPGVAAFLDEA
                     ITAGSHLAIASSATRDWLDDHLERLGIRPLFDVVCARDNGRVKPAPDIYLAALAGLKA
                     SADNAIAFEDSPNGITAAKGAGLMCVAVPNPVTAQLPLGGADLLVESFTDISFPVLVS
                     VLRGAARYAPPNV"
     gene            complement(849..1280)
                     /locus_tag="VGD55_09035"
     CDS             complement(849..1280)
                     /locus_tag="VGD55_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870531.1"
                     /translation="MAELAGYSGTPLARKLGIRDDAVLALLDAPPSWSVPELPGGVEV
                     KRGTSASRLRGAGLAEIDVALVFCRTAADVAAVPPLLGDLPKRSALWVAWPRKAGGHV
                     SDVDENLLRDVLLPTGVVDVKVAALDTDWSGLKFVWRKENR"
     gene            complement(1282..2175)
                     /gene="era"
                     /locus_tag="VGD55_09040"
     CDS             complement(1282..2175)
                     /gene="era"
                     /locus_tag="VGD55_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0870532.1"
                     /translation="MAPQHRSGFACVVGRPNAGKSTLLNALVGQKVAITSDRPQTTRH
                     AVRGIVHRPDAQLIVVDTPGLHKPRTLLGERLTDVVRTTLAEVDVIVFCVPANEKVGP
                     GDKFIAAELARLDKTPVIAVLTKTDAADRAEVATQLLALSRLGDWAEIVPVSAVAGEQ
                     VELLADLLIARLPAGPPLYLDGELTDEPEMVMIAELIREAALDGVREELPHSLAVVVE
                     EMKPREGKDLIDVFAVMYVERPSQKAIVIGAGGTRLRDVGTRARKQAEALLGTRVYLD
                     LRVKVAKDWQRDPAQLRKLGF"
BASE COUNT          365 a          746 c          734 g          333 t
ORIGIN      
        1 cgctgcgcga tgacgcgccc ggaagaatcg cgattgctgc tcattgctgc tcctcgtatg
       61 gcgggtggcg gaatacggcc ggcacgcagc aatcccgcga cgaggagacc ggcctcttag
      121 agggtctcgc cgcggcagct aaggagcagg agcctagaca ttgggaggag catacctcgc
      181 cgcaccgcgc aacacggaaa ccaataccgg aaacgagatg tcggtgaacg actcgaccag
      241 caggtcggcg ccgccgagcg gaagctgcgc cgtcaccggg ttcggcacgg cgacgcacat
      301 caggcccgcc cccttggccg cggtgattcc gttcggtgaa tcctcgaacg cgatcgcgtt
      361 gtcggccgac gcctttaagc cagcgagggc cgcgagatag atgtcgggcg ccggcttcac
      421 ccggccgttg tcacgcgcac agacaacgtc gaaaagcggc cggatgccga gccgctcgag
      481 gtgatcgtcg agccagtcgc gggtcgcgct cgacgcaatc gcgaggtgcg acccggcagt
      541 gatggcctcg tcgagaaacg ccgcgacgcc cggccgcagc ggctccacgg cggcgatcgc
      601 gttgtgccgc ttccgccagt ccgctcgcag ctcagcgtcg tcgaaatcga ctccgccctc
      661 acgtatcgcc gcgagcatcg cctcttggct gccctgcgat ccgatgtagc ggtgccaggt
      721 gtcgagcgga aaatccagtt cgtggacggc gaattgctcc cgccaggcgc gcagcgcagt
      781 cgactcggtg tcgacgagca acccgtcgaa gtcgaagatg agggcgggtg gggtcgccgt
      841 tgcgttcatc accggttctc cttgcgccac acgaacttca gccccgacca gtcggtgtcg
      901 agcgcggcaa ccttcacgtc gaccacaccg gtcggcaaca gcacgtcgcg gagcaggttc
      961 tcgtcgacat cgctcacgtg accgccggcc ttgcgcggcc acgccaccca gagtgcactt
     1021 cgtttcggga gatctcccag cagtggcggg acggcggcca cgtcagcggc ggttcggcag
     1081 aacaccagcg cgacgtcgat ttcggccaga cccgcgccgc ggagcctcga cgcggaggtg
     1141 ccgcgcttga cctcgacgcc acccggcagt tcgggcaccg accatgacgg cggcgcgtcg
     1201 agtaaggcca gcaccgcgtc gtcccgaatg ccgagttttc gcgcaagtgg cgtgcccgag
     1261 tagcccgcga gttcggccac ctcagaatcc cagcttgcgc agctgagcgg gatcgcgctg
     1321 ccagtccttg gcgaccttca cccgcaggtc aaggtagacg cgggtgccga gcagcgcctc
     1381 ggcctgcttg cgggcacgcg tgcctacatc gcgcagccgc gtaccgccgg cgccgatcac
     1441 gatggctttc tggctcggcc gctcgacata catgacggcg aagacgtcga tcagatcctt
     1501 gccctcgcgc ggtttcatct cctcgacgac cacggcgagg ctgtgcggca actcctcgcg
     1561 gacgccgtca agcgcggctt cacgaatcag ttcggcgatc atcaccatct cgggctcgtc
     1621 ggtcagctcg ccgtcgagat acaacggcgg ccccgcggga aggcgcgcga tcagcaggtc
     1681 ggcgagcaac tcgacctgct cgccggcgac cgcagagacc ggcacgatct cggcccagtc
     1741 gccgagccgc gacagtgcga gcagttgggt cgcgacctcg gcgcggtcgg ccgcatcggt
     1801 cttggtaagc accgcgatga caggggtctt atcgagccgc gcgagctcgg ccgcgatgaa
     1861 tttgtcgccg gggccgacct tctcgtttgc gggcacgcag aacacgatga cgtcgacctc
     1921 ggccagcgtg gtgcgcacga cgtcggtcag gcgctcgccg agcagggtgc gcggcttgtg
     1981 caggccgggt gtgtcgacga cgatcagctg cgcgtcgggc cgatgcacga tgccgcgcac
     2041 agcatggcga gtggtctgcg gacgatcgct ggtgatcgcg accttctggc ccacgagcgc
     2101 gttcagcagc gtcgacttgc cggcgttagg gcgccccacg acgcaggcaa atccggaacg
     2161 atgctgaggg gccacccc
//
LOCUS       DASWVF010000510         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_396029, whole genome shotgun sequence.
ACCESSION   DASWVF010000510 DASWVF010000000
VERSION     DASWVF010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396029"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGD55_09045"
     CDS             <1..253
                     /locus_tag="VGD55_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0870533.1"
                     /translation="FAEQIPALGGTMLGGEALQPTTTATSVRDDIVTDGPFVETKEAL
                     GGYYVIEAADLDQALAIAKACPAPYGGVEVRPIMEFPTG"
     gene            250..1509
                     /locus_tag="VGD55_09050"
     CDS             250..1509
                     /locus_tag="VGD55_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870534.1"
                     /translation="MTTPPEDVHRAIADAHRREWAFVLAATARVAGDIDLAEECVQDA
                     YVSALDAWTRGGVPLNPAAWLTTTARNRALDAHRRNHTLRAKLPLLVEPDVAAPEPED
                     VDHIRDDRLRLVFTCCHPALGREAQVALTLRLVCGLTTADIARAFLVTEPTMAARVTR
                     AKKKIAAARIPYRVPEAAELPDRLDAVLTVVHLLYTTGHTAPLGEQLVRADLVDRSLE
                     LARMLLALMPDEREVRGLLALILLSDARSATRVDAAGNLVLLEDQDRTKWDADRISEG
                     AALVVEALRGGGAGRFAVQAAIAAVHAEATTYADTDWPQILALYDELLRLWPSPVVAL
                     NRCVAVLMINGPAAALAELDELDATGRLAGYRYLPATRADLLRRLGRVGEAAHEYRTA
                     IDLAENDAESAFLQGRLAETLSMGSAV"
     gene            complement(1620..2636)
                     /locus_tag="VGD55_09055"
     CDS             complement(1620..2636)
                     /locus_tag="VGD55_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0870535.1"
                     /translation="MKQRTLGTGLTVSEVGYGCMSLTGAYGSTGGPTRQDAIALIRRA
                     AELGVTLFDTAEVYGPFTNEDIVGEALEPLRDQVKIATKFGFAFAENGAQPGGLCSRP
                     DSIRQAVEHSLLRLRTDHIDLYYQHRVDPDVPIEDVAGAVKELIEAGKVLHFGLSEAA
                     AGTIRRAHAVHPVTAVQSEYSMWWRDRERDTIPVLAELGIGLVPYSPLGKGFLTGTID
                     TSTTFADGDFRGQTPRFQGEALTANLALVDVLNRIAADAGATPAQLALTWLLDQQPWI
                     VPIPGTKNPQRLEENTATAAVTLTDDQLAELRAAADQIRIVGARYPEAQDAMTNRDAP
                     LPSG"
     gene            complement(2716..3126)
                     /locus_tag="VGD55_09060"
     CDS             complement(2716..3126)
                     /locus_tag="VGD55_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0870536.1"
                     /translation="MDITIHASFLPHDDPEASLAFYRDTLGFDVRLDVGKGTMRWITV
                     GPPDQPGTSIVLAPPAANPGITDDERRTIAEMMAKGTYAVLLLATKDLDGTFERLQAG
                     EAEIIQEPTEQPYGIRDCAVRDPAGNLIRIQELH"
     gene            complement(3131..3580)
                     /locus_tag="VGD55_09065"
     CDS             complement(3131..3580)
                     /locus_tag="VGD55_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0870537.1"
                     /translation="MTSPPAPAQRLRDLARLRRVRDRIDREYAQPLDVEALALGAHMS
                     TGHLSREFRLAYGESPYSYLMTRRIERAMALLRRGDLSVTEVCFAVGCSSLGTFSTRF
                     TELVGMPPSTYRTQEAGAVQGMVPCVAKQVTRPIRNREARVDAPQLA"
     gene            complement(3577..>3821)
                     /locus_tag="VGD55_09070"
     CDS             complement(3577..>3821)
                     /locus_tag="VGD55_09070"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY0870538.1"
                     /translation="HRMSGMQNAPVWVRRPVVTLVGVLLVGVGAVLLVLPGPGLLVMA
                     LGLAVLATEYRAAQRLLDRIKLPINRARARWASRRA"
BASE COUNT          587 a         1251 c         1341 g          642 t
ORIGIN      
        1 attcgccgag cagatccctg cgctgggcgg cacgatgctc ggtggggaag cgttgcagcc
       61 gacgaccacg gcgacttcgg tgcgcgatga catcgtcacc gacggtccct tcgtcgagac
      121 gaaggaagcg ctcggtggct actacgtcat cgaagccgcc gatctcgacc aggcgctcgc
      181 gatcgcaaag gcgtgtccgg ctccgtacgg cggcgtggag gttcgaccga tcatggagtt
      241 ccccaccggg tgacgacgcc gcctgaggac gtgcaccggg ccatcgccga cgcgcaccgt
      301 cgcgagtggg cattcgtgct cgccgcgacg gcgcgcgtgg cgggcgacat cgacctggcc
      361 gaggagtgcg tgcaagacgc gtacgtctcg gcgctcgacg cgtggacgcg aggtggcgtc
      421 ccgctcaacc cggcggcgtg gctgacgacc accgcccgca accgagcgct ggacgcgcat
      481 cgccggaacc acacgctgcg ggcgaaactg ccgctgctcg tcgaacccga cgtcgcagca
      541 cccgaacccg aggacgttga ccacatccgc gacgaccggt tgcgattggt gttcacctgc
      601 tgccacccgg cgctcggccg ggaggcacag gtggcactga cgctgcggct cgtgtgcggg
      661 ctcacgacgg ccgatatcgc gcgggcgttc ctggtgacgg agccgacgat ggcggcccgg
      721 gtcacgcgag ccaagaagaa gatcgccgcg gcccggattc cgtaccgcgt gcccgaggcc
      781 gcggagttgc ccgaccgact cgacgcggtg ctgaccgtcg tccacctgct gtacacgacc
      841 ggccacaccg caccgctggg ggagcagctc gttcgggccg acctcgtcga tcgatcgctc
      901 gagctggcgc gaatgttgct cgcgctgatg cctgacgagc gcgaggtccg cggtctgctc
      961 gccctgatcc tgctcagcga cgcccggagc gcaacccgtg tcgacgcagc aggcaatctg
     1021 gtgctgctcg aagaccaaga ccggaccaag tgggacgccg accggatcag cgagggcgcc
     1081 gctcttgtgg tcgaggcgct gcgtggaggg ggagccggac gcttcgccgt ccaggccgcc
     1141 atcgctgcgg tgcatgcgga agccacgacc tacgccgaca ccgactggcc gcagatcctg
     1201 gccctgtacg acgagctgct caggttgtgg ccatcgccgg tggtcgcgct gaatcggtgc
     1261 gttgcagtgc tgatgatcaa cggacccgcg gccgccctcg ccgagcttga cgagctcgac
     1321 gcgacaggtc ggctcgccgg ctaccggtac ctgccagcaa cgcgcgctga tctgttgcgc
     1381 cggcttggcc gcgttggcga ggccgcgcac gaataccgca ccgcgatcga cctggccgag
     1441 aacgacgccg agagcgcgtt ccttcagggg aggctcgctg agaccttgtc gatggggtca
     1501 gcagtctagg aatcagtacg acttgacata atgtacctta tcggcactta agagggaacg
     1561 gggtgccgta ggaaccgact cggtctcaac gagtcccgta ctggccccct tggcgccatc
     1621 tagccgctcg gcagcggcgc gtcacggttg gtcatggcgt cttgtgcctc cgggtaacgg
     1681 gcgccgacga tgcggatttg gtccgccgcg gcccgcagtt ccgcgagttg gtcgtcggtc
     1741 agcgtcaccg ctgcggttgc agtgttctcc tcgagccgtt gcgggttctt ggtgccgggg
     1801 atcggcacga tccagggctg ctggtcgagc agccaggtca gcgcgagctg tgctggtgtg
     1861 gcacccgcgt ccgcggcgat gcggttgagg acgtcgacga gcgccaggtt ggcggtgagc
     1921 gcctcgccct ggaaacgcgg tgtctggccg cggaagtcgc catccgcgaa cgtggtgctc
     1981 gtgtcgatgg tgccggtcag gaatcccttc cccagtgggc tgtagggcac gagcccgatg
     2041 ccgagctccg ccagcacggg aatggtgtcc cgttcgcggt cacgccacca catcgagtac
     2101 tcgctctgca ctgccgtgac cgggtggacg gcatgtgccc tgcggatcgt gccggcggca
     2161 gcctctgaca gaccgaaatg cagcaccttg ccggcctcga tcagttcctt gactgcgccg
     2221 gcgacgtcct cgatcggcac gtccgggtcg acgcggtgct ggtagtacag gtcgatgtgg
     2281 tccgtgcgga ggcggagcag cgaatgctcc acggcctggc ggatgctgtc cggacggctg
     2341 cacagcccgc ccggctgagc gccgttctca gcgaaggcga agccaaactt ggtcgcgatc
     2401 ttgacctggt cgcgcagggg ttccaaggcc tcaccgacga tgtcctcgtt ggtgaatggt
     2461 ccgtacacct cggcggtgtc gaacaaggtc acgccgagtt cggcggcgcg gcggatcagc
     2521 gcgatcgcgt cctgccgggt cgggcctccc gtgctgccgt aggcgccagt caggctcatg
     2581 cagccgtagc cgacctccga gacagtcagc ccggtgccca aggttcgctg tttcatgaca
     2641 gttccttcag tagttactag ctgactgggt ccccgatagt ggtgatcctt cgagtgggag
     2701 gggacggggc ttcgctcagt gcaactcttg gatgcggatc aggttgccag cggggtcgcg
     2761 gacagcgcaa tcgcgaatcc cgtacggctg ctcggtcggt tcctggatga tctcggcctc
     2821 gcctgcctgg aggcgctcga acgtgccgtc cagatccttg gtggccagca gcagcacggc
     2881 gtaggtgccc ttggccatca tctcggcgat cgtgcgtcgc tcgtcgtcag tgatgcccgg
     2941 attggcggcc ggcggggcga gcacgatgga cgtgcccggc tggtcgggcg gcccgaccgt
     3001 gatccagcgc atcgtccctt tgccgacgtc gaggcggacg tcgaagccga gggtgtcgcg
     3061 atagaaggcc agcgatgctt cggggtcgtc gtgcgggagg aagctcgcgt gaatggtgat
     3121 gtccatagcg tcaggctagt tgcggcgcgt cgactcgtgc ttctcgattc ctgatcggtc
     3181 tggtgacttg tttcgccacg cacggcacca tcccttgcac cgccccggct tcctgggtgc
     3241 ggtacgtgct cggcggcatg cctaccaact cggtgaagcg ggtgctgaac gtgccgagcg
     3301 acgagcagcc gaccgcgaag cacacctcgg tgacgctgag gtctcccctg cgcagcagcg
     3361 ccatcgcgcg ctcgatgcgt cgcgtcatca ggtagctgta cggcgattcg ccatatgcga
     3421 ggcggaattc acgactcagg tgccctgtcg acatgtgcgc gccgagcgcg agcgcctcga
     3481 catcaagggg ctgcgcatat tcgcggtcga tcctgtcgcg cacccggcgc agccgtgcga
     3541 ggtcacgcag gcgctgcgcg ggggctggcg ggctggtcat gcgcgccggc ttgcccagcg
     3601 ggcgcgcgcc ctattgatcg ggagcttgat gcggtcgagc aagcgctggg cggcccggta
     3661 ttcggtcgca agcactgcga gaccaagggc catcaccaac aggcctgggc cagggagcac
     3721 gagcagcacg gcgcccaccc cgaccagcag gacgccgacc agcgtgacca cagggcggcg
     3781 aacccagacg ggtgcatttt gcatgccact catccggtgc a
//
LOCUS       DASWVF010000511         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_39113, whole genome shotgun sequence.
ACCESSION   DASWVF010000511 DASWVF010000000
VERSION     DASWVF010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39113"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1168)
                     /gene="prcA"
                     /locus_tag="VGD55_09075"
     CDS             complement(416..1168)
                     /gene="prcA"
                     /locus_tag="VGD55_09075"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEY0870539.1"
                     /translation="MSAPFYVSPEQLMKDRADYARKGIARGRSNVVLQYADGILFVAE
                     NPSKALHKISEVYDRIAFAAVGRYNEFENLRVAGVRLADLTGYTYDRSDVTARTIANA
                     YAQTLGAIFSGAGEKPYEIEIVVAEVGESEDDDTIYRLTYDGSVTEEHAFVAMGGQAE
                     HISGLLKERFVADSSLTAALRLAVDVLTQAPDGEMRTLTAGQLEVAALVRSRARRAFR
                     RFVGPELEALLAASSAHQAELPDADVDPDVES"
     gene            complement(1226..2071)
                     /gene="prcB"
                     /locus_tag="VGD55_09080"
     CDS             complement(1226..2071)
                     /gene="prcB"
                     /locus_tag="VGD55_09080"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEY0870540.1"
                     /translation="MTTDGSGRLPTAFHQPGVGSFADFLGMHAPDMMPGKRTLGPGTV
                     DAPHGTTIVAVTYTGGVVMAGDRRATMGNIIAQRDIEKVFQADEHSCVGIAGTAGLAI
                     ELVRLFQVELEHYEKLQGATLSLEGKANRLSMMIRGNLPMAMQGLAVVPMFAGYDLLV
                     SDPEKAGRIFSFDVTGGRYEEHSFHAVGSGSMFARGALKKFFREDFSQQDAVRACVEA
                     LYDAADDDSATGGPDLTRQIYPVVVSCSIEGFQRVADDILHDMVGRVVDARMIRPGGP
                     AAPLS"
BASE COUNT          340 a          683 c          694 g          364 t
ORIGIN      
        1 agccgcgggc gacgagcgcc tcttcatagg actgtgcggc gacacgcttc tcgttcacca
       61 gccggtcgac gacctcggga ggtgatgcgt tctcctgtgc caggtccagc cgtgccacgg
      121 cggcaaggta cgcccgcatc aatccgtgga gccgtgcatc gttcgtccag gacgccatgt
      181 caccctcctt cgcagacccg ggcggcggct gcggatctcc ccgcagttcc accaatcacc
      241 cgtcgtttcc ccagtcacag agagtgatcg gccaattgga ccagtgaact tgagcccaat
      301 cggcggaaag attcgccgcg gctggctcag cgctcaggcg ccgttggctt agtgccgttg
      361 cgccggttgc gcagcgtaac cgcgcagctg gctcagtgcg ggcctggacg ccgtctcagg
      421 actcgacgtc ggggtccaca tcggcgtcgg gtagctcggc ttgatgggcg gagctcgccg
      481 ccagcagcgc ctcgagctcg gggccgacaa agcggcggaa tgcgcgccgt gcgcgcgacc
      541 tgaccagcgc ggcgacctcg agttgcccgg cggtcagcgt ccgcatctcg ccgtctggcg
      601 cctgcgtgag cacgtcaacc gccagccgca aggcagcggt gagcgaggag tcggcgacga
      661 aacgctcctt cagcaggccg ctgatgtgct cggcctgacc gcccatggcc acgaacgcgt
      721 gctcctccgt gacagagccg tcgtaggtca gccgatagat cgtgtcgtcg tcctcggact
      781 caccgacttc ggccaccacg atctcgatct cgtagggctt ctcccccgcg ccgctgaaga
      841 tcgcacccag cgtctgggcg taggcgttcg cgatcgtccg agcggtcacg tcactgcggt
      901 cgtaggtgta gccggtcagg tcggcaagcc ggacgccggc aacccgcagg ttctcgaact
      961 cgttgtagcg accgaccgcc gcgaacgcga tccggtcgta gacctcactg atcttgtgca
     1021 gcgccttcga aggattctcg gcaacgaaca agatgccatc ggcgtactgg agtaccacgt
     1081 tgctgcgacc gcgcgcgatg cccttgcggg catagtcggc ccggtccttc atcagttgct
     1141 cgggcgacac gtagaacggc gcgctcatgt gctgctcctg gtcggtgtct ggtgtttcac
     1201 gggtgaggtc agctcggtag ttcgcttagc tgagcggtgc ggctgggccg ccgggccgga
     1261 tcatgcgggc gtcgaccacg cggccgacca tgtcgtgcag gatgtcatcg gcgactcgct
     1321 ggaagccctc gatcgagcaa gacacaacga ccgggtagat ctgacgggtg aggtcaggac
     1381 cgccggtcgc tgagtcgtcg tcggccgcgt cgtacaacgc ctcgacgcac gcgcggacag
     1441 cgtcttgctg gctgaagtct tcgcggaaga acttcttcag ggcgccgcgg gcaaacatgg
     1501 atcccgagcc gaccgcgtgg aacgagtgct cctcgtagcg gccgccggtg acgtcgaacg
     1561 agaagatgcg accggccttc tcggggtcgc tgacgagcaa gtcgtagccg gcgaacatcg
     1621 gcaccacggc gaggccctgc atcgccatcg gcagattgcc acggatcatc atcgagagcc
     1681 gatttgcctt gccctcaagg gaaagtgtcg cgccttggag cttttcgtag tgctcaagtt
     1741 ctacctggaa cagtcggacg agttcgatcg ccagaccggc tgtaccggcg atgccgacgc
     1801 atgagtgctc gtcggcctgg aacacctttt cgatgtcgcg ctgggcaatg atgttgccca
     1861 tcgtggcgcg gcggtcgccg gccatcacga caccaccggt ataggtgacc gcaacgatcg
     1921 tcgtgccgtg cggcgcgtcg accgtgcccg ggccgagggt gcgcttgcca ggcatcatgt
     1981 cgggcgcgtg catgccgagg aagtcggcga aggagccgac gcccggctgg tgaaacgcgg
     2041 tcggcagccg acctgaaccg tcggtcgtca ctggccaccc t
//
LOCUS       DASWVF010000512         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_268752, whole genome shotgun sequence.
ACCESSION   DASWVF010000512 DASWVF010000000
VERSION     DASWVF010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268752"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..357)
                     /locus_tag="VGD55_09085"
     CDS             complement(37..357)
                     /locus_tag="VGD55_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY0870541.1"
                     /translation="MSRRPRLWCRVVVTGDSMLPALRAGDCLLVAVRARVHAGDVVVA
                     RRPAVPGLLLVKRAIRRADVGWWLLSDNAAEGLDDSRVFGVLPDDAVVGKVLFRYYPW
                     RRRR"
     gene            466..867
                     /gene="sodN"
                     /locus_tag="VGD55_09090"
     CDS             466..867
                     /gene="sodN"
                     /locus_tag="VGD55_09090"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY0870542.1"
                     /translation="MLARILTPKTVVHAHCDLPCGVYDPAQARIEAESVKAVCEKYQA
                     NEDPAFRTRALIIKEQRAELVKHHLWVLWTDYFKPVHLETYPQLHTLFWEATKLAGAA
                     GTKGQADPAVAQQLLDKIAEIDTIFWATKKA"
     gene            complement(876..2030)
                     /locus_tag="VGD55_09095"
     CDS             complement(876..2030)
                     /locus_tag="VGD55_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0870543.1"
                     /translation="MASIAKRLDGQWRARYRDDAGKEHARHFGRKVDAQRWLDDVTAA
                     IVTGQYVDPKAGRTTVAEYAKVWQAAQVGSPGTARIVDNAVRLHIVPRLGTTPLAAVR
                     RGDAQALVKALSMTYAAGTVRNVYDVLARIMASAVDDRLIAATPCRRIVLPRIDDGEI
                     SPPAVEDILALAAAVPRRYRAVVVTLAGSGLRIGELLGLSVADVDFLRRTVRVERQRT
                     QSGELTAPKTGKSVRTVPLGQVVIDELAAHLAAFPSAEWLFTVDGDEPLQYRSWKWIW
                     RTAHAARQRAEVAAAAKQERKSVTFAAVGTHDLRHFYASALIAGGATVKQVQTVLGHA
                     SAMITLRTYAHLWPGDDDRTRAVMDSTLDVLRTARGLAATSGGIAAGQEA"
     gene            complement(2033..>2336)
                     /locus_tag="VGD55_09100"
     CDS             complement(2033..>2336)
                     /locus_tag="VGD55_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870544.1"
                     /translation="EYITAAATSKIVKEEGGVVTSVVQVDDQAQRNAINEIRDARRSG
                     RRRVTPDLLVRVAAVHADHADRPVEAVQDAFQTAYRTAARYVQLARAQGLIPPKEH"
BASE COUNT          378 a          806 c          769 g          383 t
ORIGIN      
        1 gctggctgcg ggccggcggc cgtcgagcgc tgaaccctat cggcggcggc gccaggggta
       61 gtagcgaaac aacaccttgc cgacaacggc gtcatcgggc aggacgccga agacgcggct
      121 gtcgtcgagg ccttcggcgg cgttgtccga caacagccac cagccgacgt ccgcccgacg
      181 tattgccctt ttgacgagaa ggagtcctgg aacggccgga cggcgggcga ccacgacgtc
      241 gccggcatgc acccgagcgc ggacggcgac cagcaggcag tcacccgccc gcaacgccgg
      301 cagcatcgag tcaccggtga cgacgacgcg acaccataaa cggggacgtc ggctcattca
      361 gtgatcacct cccggcaggt tgcaggccac gatgccaggg gtagtgtcca gcgagatcag
      421 cagatcgacg tccggacagg tacctgcgga ggaagcccca tgaagctgct cgcccgaatt
      481 ctcacgccca agaccgtggt tcacgcgcat tgcgacctgc cgtgcggcgt ctacgacccg
      541 gcacaggcac gcatcgaagc tgagtcggtc aaggccgttt gcgagaagta ccaggcaaac
      601 gaagaccccg cattccgcac gcgggccctg atcatcaagg agcagcgcgc cgaactcgtc
      661 aagcatcacc tgtgggtgct gtggaccgat tacttcaagc ctgtgcatct cgagacctac
      721 ccgcagctgc acaccctgtt ctgggaggcc acgaagttgg ccggtgcggc aggtacgaag
      781 ggccaggccg acccggcggt cgctcagcag ctcctcgaca agattgccga gatcgacacg
      841 atcttctggg ccaccaagaa agcctaggta ccacctcagg cctcctgacc tgcggcgatg
      901 ccgccggacg tggccgcgag tccgcgcgca gtccgcaaga catccagtgt cgagtccatg
      961 acggcccgag ttcggtcgtc gtctccgggc cacagatggg cgtaggtccg cagcgtgatc
     1021 atggcgctcg cgtgaccgag gaccgtctgg acctgcttca ccgtggcgcc tccggcgatc
     1081 agcgccgagg cgtagaagtg gcgtaggtcg tgcgtcccga cagcggcgaa tgtcaccgac
     1141 ttgcgctcct gcttggccgc tgcggcgacc tctgctcgtt gccgcgccgc atgcgccgtt
     1201 cgccagatcc acttccagga ccggtactgc aacggctcgt cgccgtcgac cgtgaagagc
     1261 cactcggcgc tcgggaatgc cgcaagatgt gccgccagct cgtcgatgac cacctggccg
     1321 aggggcaccg tccggaccga cttgcccgtc ttcggcgcgg tcagctcgcc gctttgggtc
     1381 cgctggcgtt cgacgcgcac ggtgcgacgc aggaagtcga cgtcggcaac actcaggccg
     1441 agcagctcgc cgatccgcaa cccggagcca gccagcgtga caacgacggc tctgtaccgc
     1501 cgcggcacag cggctgctaa cgcgagtatg tcttcgacgg ccggcggcga gatctcgccg
     1561 tcatcgattc gaggtagcac gatccgccgg cacggcgtgg ccgcgatgag ccggtcgtcg
     1621 accgcgctcg ccatgatccg cgcaaggacg tcgtacacgt tcctgaccgt gccggcggcg
     1681 taggtcatag acagtgcctt gacgagtgcc tgcgcatcgc ctcgtctcac cgcggcgagc
     1741 ggtgtggtcc cgagtcgggg aacgatgtgg agtcgcaccg cattgtcgac gatccgcgcc
     1801 gtgccagggc tgccgacttg cgccgcctgc cacaccttcg cgtactcggc gaccgtcgtt
     1861 cggcccgctt tggggtcgac gtactggcct gtgacgatcg ccgccgtgac gtcgtcgagc
     1921 caccgctggg catcgacttt gcgtccgaaa tgccgcgcgt gctctttgcc ggcgtcgtct
     1981 cggtagcgcg cacgccattg gccgtccagt cgcttcgcga tgcttgccac gatcagtgct
     2041 ccttcggtgg gatcaggccc tgcgctcgcg cgagttgcac gtatcgggcg gctgtccggt
     2101 aggccgtctg gaacgcgtcc tgcacggctt cgacgggccg gtcggcgtgg tcggcgtgga
     2161 cggccgccac ccgcacgaga aggtccggag tgacccgtcg gcggccgctg cggcgagcgt
     2221 cgcggatctc gttgatcgcg tttcgctgcg cctggtcgtc gacctgcacc acgctcgtca
     2281 ccacgccccc ctcctccttc acgatcttcg acgttgcggc cgcagtgatg tattcg
//
LOCUS       DASWVF010000513         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_396126, whole genome shotgun sequence.
ACCESSION   DASWVF010000513 DASWVF010000000
VERSION     DASWVF010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396126"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VGD55_09105"
     CDS             complement(<1..712)
                     /locus_tag="VGD55_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEY0870545.1"
                     /translation="MFRCRTSCRPVLCRAAVVSVAIVLFGRALTANADEAWGDRVEEA
                     PQPGSAPLYLRTALEELGFLGLQTAWYWGHSFHGDEKFNWHNWSTRLTSTHDMVLDDD
                     KFRTGAVGHPIAGAGYYLIARGNGFNAISSFAITVLASTTWQYFSEWNEKPATNDLLV
                     TPVAGWAIGEASFRLGQYFIERGPGVFGCVGAALFSPVATYNDARPCRFHRGDAGEEE
                     GQERRPDLGHRFDLGVGTA"
     gene            1101..3089
                     /locus_tag="VGD55_09110"
     CDS             1101..3089
                     /locus_tag="VGD55_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870546.1"
                     /translation="MACQSLERRRCGPTGAGLLPSLVLVAGLAGSCSFKPSGTPAPVA
                     GGGGNAGLAVDGGTATTGGGNLPGVTSLQLSPAMASIAVTNSAPVATQQYTVTGTVGG
                     HSEDLTSMVAYSATPPGIVKVDNTGLATATGTAGGRVTITASTGSVSAQATLMVTYSF
                     TGADPGAAGTGFPANAQAIFSGAPADPSRAPKLVYPNDGVLFPPNVSGIEVHFTPGKN
                     NTLFEVSLSGPLSTVTTFVRCTAPNGIKGCIYLPAPSLWASVARGNAGQGPVTVKVSG
                     TDDTGTGAGTSSPITMRFAQDDIQGALYYWTTSGQSAIERWDFAGGSAATAYLTPTNT
                     DGHTCVGCHALSPNGDKLVASAGGENDGRLLLWNVSANTALQPFPLVQKSQFESWNAD
                     GTKFVGVFGDSLPGQAGPVSLMIFDGTSGAVTSTIDLGGLRADHPDWSKNTGGPDTIV
                     FTAADPAAATPDQQPANGGISYVRMSNGSWGPPATLVPTQLGKNRYYPAISPDGNLVV
                     YDESTCAKTPRLGQTPDSSCNGDVDPTATMFVTTFSGGAPVPLTRANSPGVADGTTTA
                     LTNSFPRWAPFVERLDEMHQLVWLTFSSTRQYGLRSPPPSTAPDEAVASTLIWMVGVD
                     LDAGGSDPSYSAFCLPFQDVTTSNHIAQWAKYFIQGPG"
     gene            3141..>3815
                     /locus_tag="VGD55_09115"
     CDS             3141..>3815
                     /locus_tag="VGD55_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY0870547.1"
                     /translation="MLSLLSTQSGQRGWKRHAVILRVGVLLAAAAFLAGRVRWREVGT
                     TVLHARAGLLGTVILLNGSILIIKAVRLRLMTAGRLSVSTSILTKVAVAALNNLSPLR
                     GGDIARLWMLERHAGMTRTSGLAVGVSELLVEVTTLCSLVALIAGALPGQRWALIAAL
                     VVLGPAVALLIVLPRARSALRDGLRPLRNLKTVGVIVLLSSAEWLIESGMIVVTAKAM
                     GLNIGFA"
BASE COUNT          660 a         1237 c         1245 g          673 t
ORIGIN      
        1 gggcggtgcc gaccccgaga tcgaaccggt ggccgaggtc ggggcgacgc tcctgaccct
       61 cttcctcacc ggcgtcccct cggtgaaagc gacacgggcg ggcgtcgttg tacgtcgcga
      121 ccggcgaaaa gagcgcggcc ccgacgcacc cgaagacccc cggccctcgt tcgatgaaat
      181 actgtcccag ccggaagctc gcctcaccga tggcccaacc tgccacgggc gtgaccagta
      241 gatcgttcgt cgccggcttt tcgttccatt cgctgaagta ctgccaggtc gtcgacgcca
      301 gcacggtgat cgcgaacgac gagatcgcgt tgaacccatt gccacgggcg atgaggtagt
      361 agccggcccc cgcgatcgga tgcccgacgg cgccggtccg gaacttatcg tcgtcgagca
      421 ccatgtcgtg agtgctggtc aggcgcgtgc tccagttgtg ccagttgaac ttctcatcgc
      481 cgtggaagct gtgtccccag taccaggccg tctgcaagcc gaggaacccc agctcctcca
      541 gtgcggtacg caggtacagg ggcgccgatc cgggctgtgg cgcttcctca acacggtcac
      601 cccacgcttc gtcggcgttc gctgtcagtg cgcggccgaa tagaacgatc gcgacggaaa
      661 cgactgctgc gcggcacagg accggccgac acgacgtgcg acatcgaaac atctgcgccg
      721 aggtccacca accggtagct ccccgagatc acagttctca ccggagtgat gcgcacaagt
      781 gagcggtcac gccggccgcc aactagcaga gatcggctga cgcgcctccg gcgcatcgca
      841 actggcggca cgatgtccgc cgttcgcttt cagccacgcc ggccgttcat gtccggcgaa
      901 cgcaaccgca ggaggagtaa ggatggaaac ccggggtcga tcggtgagtt tccttcaggt
      961 caaggccgtg accccacctg gtgcccactg gtgtgggtgg tcgagagtgg gtaaccgacg
     1021 cctcctcggt gtgcgcggcg ccgtgatggc agcgagcagc tcaagcaacg ccagtcgtgg
     1081 cgccgcatac caaccccctc atggcatgtc agtctctcga gcgccgtcga tgtgggccaa
     1141 cgggcgcggg cctgctaccg tcgctggtgc tggtcgcagg gctcgcagga agctgctcgt
     1201 tcaaacccag cggcacaccc gcccccgtcg caggcggagg gggaaacgcc gggttggccg
     1261 tcgatggtgg caccgcaacc actggcggtg gcaaccttcc cggagtcact tctcttcagc
     1321 tcagtccggc tatggcatcc atagcggtga cgaatagcgc accggtcgcg acccagcagt
     1381 acacggtgac cgggacggtc ggcggtcatt cagaagatct aacttcgatg gttgcttact
     1441 cagccacgcc gcccggaatc gtgaaggttg acaacacggg actggcgaca gcgacgggta
     1501 cggctggcgg acgcgtcacg ataaccgcct cgaccgggag cgtctcagcg caggcgacat
     1561 tgatggtgac gtattcgttc accggcgcgg atccgggtgc cgcggggacc ggtttcccgg
     1621 caaacgcgca ggcgatcttc tcgggagcgc cggccgatcc gagccgtgcc ccaaagctgg
     1681 tgtatccgaa cgacggggtc ctgtttccgc ccaacgtgag cgggatcgag gttcacttca
     1741 cgccggggaa gaacaacacg ctgttcgagg tcagcttatc cggtccgctc tcgacggtga
     1801 ccaccttcgt gcggtgcacg gccccaaacg gaatcaaagg gtgcatctac ctgccggctc
     1861 cctcgctctg ggcatccgtg gcccgcggga acgctggcca gggccccgtg acggtcaagg
     1921 tgagcggaac ggacgacacg ggaaccgggg ctggtaccag ttcgcccatc acgatgcgct
     1981 ttgcccagga cgacatccag ggggcgctct attactggac gacgagcggt caaagcgcga
     2041 tcgagcggtg ggatttcgcg ggtggctcgg cggcgacggc ctacctgaca ccgacgaaca
     2101 ctgatgggca tacctgcgtc ggctgccacg ctttgtcccc gaatggcgac aagctggtcg
     2161 caagcgccgg cggcgagaac gacggccggc tgttgctttg gaacgtcagc gccaacactg
     2221 ccctgcagcc cttcccgctg gtccagaaga gccaattcga atcgtggaac gccgacggca
     2281 cgaagttcgt cggcgttttc ggcgacagct tgcccggtca ggcaggaccc gtgagcttga
     2341 tgatcttcga tgggacgagc ggcgcggtca cgtccaccat cgatctgggt ggcctgcgcg
     2401 ccgatcaccc cgactggtcg aagaacactg ggggaccgga caccatcgtg ttcacggctg
     2461 ccgatccggc agctgcgacg cccgatcagc agccggcgaa cgggggaatc agttatgtca
     2521 ggatgagcaa tgggtcttgg ggcccacccg cgactcttgt gccgacgcaa ctcggcaaga
     2581 acaggtacta cccagcgatc tcgccggacg gcaatctcgt cgtttacgac gagtccacct
     2641 gcgcgaagac cccaaggctg ggacagacgc ccgattcgtc ctgcaacggc gacgtcgatc
     2701 cgacggccac gatgttcgtg accacgtttt cgggcggtgc cccggtccca ctgacccgcg
     2761 cgaacagccc gggtgtggcg gatggtacga caaccgccct caccaactcc ttcccgcgat
     2821 gggcgccgtt cgtcgagagg ctcgatgaaa tgcatcagct cgtgtggctg acgttttcgt
     2881 cgacgcgaca atacgggttg cgttcgccgc cgccatcgac ggcccccgac gaggcggtgg
     2941 cgagcaccct catctggatg gttggcgtcg acctggatgc cggcggctca gaccccagct
     3001 actcggcgtt ctgtttgccg ttccaggacg tgacgacatc gaaccacatc gcgcagtggg
     3061 cgaagtactt tattcagggc cccggttgaa ttgcggctag agtcgcgacg ccgatcgtct
     3121 cgcggcgctt ggatcctgat atgctgtcgc tgttgtccac ccagtcaggc cagcgcgggt
     3181 ggaaacgtca cgccgtcatc ctcagggtcg gcgtcctgct cgcggcggcg gcattcctgg
     3241 ccggccgtgt gaggtggcgc gaggtcggaa cgaccgtgtt gcacgcgcga gcaggactgc
     3301 tcggcaccgt catcctgctc aacggatcga tcttaatcat caaagcggtg cggctgcgcc
     3361 tcatgaccgc aggtcggctt tcagtctcga cgagcatcct gaccaaggta gccgtcgcgg
     3421 cgctcaataa cctctctccg ctacgcgggg gcgacatcgc gcgtctgtgg atgctcgagc
     3481 ggcatgcggg gatgaccaga acgtccggcc tcgcggttgg tgtctcggaa ctgctggtcg
     3541 aggtcacgac cttgtgcagt ctcgtcgcgc tgattgccgg cgcgctacct ggccagcggt
     3601 gggcgctcat cgccgcgctt gtcgttcttg gccctgcggt ggccctcctc atcgtgcttc
     3661 cgagggctcg ttcggcgctt cgggacggtt tgcgacccct gcggaatctg aagaccgtcg
     3721 gagtcatcgt tctgctgtcg agcgcagagt ggctgattga atcagggatg atcgtcgtga
     3781 cggccaaggc catgggtctc aacatcgggt ttgct
//
LOCUS       DASWVF010000514         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_141422, whole genome shotgun sequence.
ACCESSION   DASWVF010000514 DASWVF010000000
VERSION     DASWVF010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141422"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /gene="rpsI"
                     /locus_tag="VGD55_09120"
     CDS             <1..235
                     /gene="rpsI"
                     /locus_tag="VGD55_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0870548.1"
                     /translation="ALDGVEQFDIVCRITGGGISGQAGALRLGIARALILIDPENRPT
                     LKKAGYLTRDARVIERKKYGLKKARKAPQYSKR"
     gene            240..1625
                     /gene="glmM"
                     /locus_tag="VGD55_09125"
     CDS             240..1625
                     /gene="glmM"
                     /locus_tag="VGD55_09125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0870549.1"
                     /translation="MPDNSVRVMDAEQTRFRLFGTDGVRGVANQELTAELALELAVAA
                     AHVLGEAGAFEGHRPLAVVGRDPRASGEFLEAAVVAGLASAGVDVLRLGVLPTPAVAY
                     LTADLKADLGVMLSASHNPMPDNGIKFFARGGHKLSDELEDAIEARLLEEWQRPIGAG
                     VGRVHDYPEATDRYVDYVVSTVPNRLDGLKVVVDCANGAASFAAPEALRRAGATVIAI
                     GSEPDGLNINEGCGSTHLELLSEAVLAHRADVGVAHDGDADRCLAVAADGSVIDGDQI
                     LAILSIAMNESGRLARGTVVATVMSNLGFRLGMEAAGIKVVETAVGDRYVLEAMRADG
                     YTVGGEQSGHVILLDYATTGDGILTALQLLARITKTKQSLLELAAVMHRLPQVLINVT
                     DVDRTRAETDAELLAAVERASNDLGTSGRILLRPSGTEPVVRVMVEAATRDQANAVAD
                     QLARVVRERLG"
     gene            1629..>2189
                     /locus_tag="VGD55_09130"
     CDS             1629..>2189
                     /locus_tag="VGD55_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEY0870550.1"
                     /translation="MCGIVAYVGPQPARDVVLSGLRRLEYRGYDSAGIAVLDAGLVHV
                     RRKAGALSNLEVVLETDPLPAGGTGLGHTRWATHGRPTDDNAHPQIDCTGGLALIHNG
                     VIENHAQLRARLIKDGHTFRSQTDTEVAAHLLEEAFASCGDLAESLRRVCRILDGSFS
                     FVAVHRHAPGLVVGARRNSPLVLGVGD"
BASE COUNT          355 a          734 c          727 g          373 t
ORIGIN      
        1 agcgctcgac ggcgtcgagc agttcgacat cgtgtgccgc atcaccggcg gtggcatcag
       61 cggccaggcc ggtgcgcttc gcctcggcat cgcccgcgcg ctgattctga tcgatcccga
      121 gaaccgcccg accttgaaga aggccggcta cctcacccgt gacgcccgtg tcatcgagcg
      181 taagaagtac ggtctgaaga aggcccgcaa ggctccgcag tactccaagc ggtaaacgcg
      241 tgcccgacaa ctcggtgcgc gtcatggatg cggagcaaac acgcttccgg ctgtttggta
      301 ccgacggtgt tcgcggtgtc gcgaaccagg aactcaccgc cgaacttgcc ctcgaactcg
      361 ccgtcgctgc agcccacgtc ctcggcgagg ccggcgcctt cgagggtcat cgaccgctcg
      421 cggtcgtggg ccgcgaccca cgtgcatccg gtgaattcct cgaggccgct gttgtcgccg
      481 gcttggccag tgcaggcgtc gacgtgttgc ggctcggcgt cctcccaacg ccagccgttg
      541 cgtacctgac ggctgacctg aaggccgacc tcggcgtcat gctgtcggcg agccataacc
      601 cgatgcccga caacggcatc aagttctttg cgcgtggcgg ccacaagctt tccgacgaac
      661 tcgaagatgc aatcgaggca cggctgctcg aagagtggca gcgtccgatc ggggccggcg
      721 tcgggcgcgt gcatgactac cccgaggcga ccgatcgcta cgtcgactac gtcgtctcca
      781 ccgtgcccaa ccggctcgac ggcctcaaag tcgttgtcga ctgcgcgaat ggtgcggctt
      841 ccttcgcggc tccagaggca ctgagacgag cgggcgcaac ggtgattgcg atcggcagcg
      901 agcccgacgg cctcaacatc aacgaagggt gtggctcgac gcacctcgag ttgctctccg
      961 aggcggtcct tgcacaccgt gccgatgtcg gcgtcgcgca cgacggcgac gcagaccgtt
     1021 gccttgccgt tgcagccgac ggatccgtca tcgacggcga ccagattctg gccatcctgt
     1081 cgatcgcgat gaatgagtca gggcggctcg cgcggggcac ggtcgttgcg accgtcatgt
     1141 cgaatctcgg gttccggctc ggcatggagg ccgcgggaat caaggtcgtg gagacggcgg
     1201 tcggtgaccg ttacgtcctc gaagcgatgc gcgccgacgg ttacacagtc ggcggcgagc
     1261 agagcgggca cgtgatcctg ctcgactacg cgacgactgg tgacggcatt ctgaccgcgc
     1321 tgcagttgct cgcacgcatc acaaagacca agcagtcgct gctcgaactc gccgcggtca
     1381 tgcaccggtt gccgcaggtg ctcatcaacg tcaccgacgt cgaccgcacg cgtgccgaga
     1441 ccgacgccga gttgctcgcg gctgtcgagc gcgcaagcaa cgacctcggt acatccggcc
     1501 gcattctgct gcgccccagc ggaaccgagc cggtagtgcg ggtgatggtc gaggctgcca
     1561 ctcgcgacca ggccaacgct gttgccgacc agctagcccg ggtcgtgcgc gagcggttgg
     1621 gttagaccgt gtgcggcatc gtggcctacg tcggaccgca gccggctcgc gacgtcgtcc
     1681 tctccggtct tcggcggttg gagtaccgcg gctacgactc ggcaggcatc gccgtgctgg
     1741 acgccggttt ggtgcacgtc cggcgcaagg ccggagcgtt gtcgaacctc gaggtcgtgc
     1801 tcgagacgga tccgctgccc gctggcggta ccggtctagg gcacacccgc tgggctacgc
     1861 atggccggcc gacggacgac aacgcgcacc cgcagattga ctgcaccggc gggctcgcct
     1921 tgattcacaa cggtgtcatc gagaaccatg cccagctgcg ggctcggctg atcaaagacg
     1981 gccacacgtt tcgatcgcag acggacaccg aggtagcggc tcacctgctc gaggaagcgt
     2041 tcgcttcgtg cggcgatctc gcggaatcgc tcaggcgcgt ctgccggatt ctcgacgggt
     2101 cgttcagctt cgtcgccgtc catcgccacg cacccgggct tgtcgtcggt gcgcggcgca
     2161 actcgccgct ggtgctcggc gtcggcgat
//
LOCUS       DASWVF010000515         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_243219, whole genome shotgun sequence.
ACCESSION   DASWVF010000515 DASWVF010000000
VERSION     DASWVF010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243219"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /gene="fabI"
                     /locus_tag="VGD55_09135"
     CDS             complement(<1..633)
                     /gene="fabI"
                     /locus_tag="VGD55_09135"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0870551.1"
                     /translation="MGLLAGKRLLITGVLTEASIAFDAARLAQQEGAHVVLSSYGRVF
                     RLTERIAGRLPEPPPVIELDVTNVDDLAALPERVGAHLDGLDGVVHAIAFAPGDALGG
                     NFLNTGWDDVATALHVSAFSLKALTMAALPMMPGGGSVVGLDFDAKVAWPLYDWMGVA
                     KAALESTSRYLARDLGKQNIRVNLVSAGPIRTMAAKGIPGFDELAAIWETR"
     gene            complement(634..1338)
                     /gene="fabG"
                     /locus_tag="VGD55_09140"
     CDS             complement(634..1338)
                     /gene="fabG"
                     /locus_tag="VGD55_09140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY0870552.1"
                     /translation="MPRSVLVTGGSRGIGLAAAHAFAAQGDRVAVTYRSSEAPQGFFA
                     IHCDVTKPDDVESAFELVEAEHGPVEVLVANAGITRDTLLLRMTDDDWDDVIATNLTG
                     AYRVARRAARAMLRNHGGRMIFVSSVVGLSGSAGQANYAATKSGLVGFARSLAREFGS
                     RGITANVVAPGFVETDLTAVLREPRRAELLDQIPLKRYATTDEIAGVIRFLASDEAAY
                     ITGAVIPVDGGLGMGH"
     gene            1641..2018
                     /locus_tag="VGD55_09145"
     CDS             1641..2018
                     /locus_tag="VGD55_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0870553.1"
                     /translation="MSTMTADTLGTVSANVLADGIIVRLNGSLDVASLPDLRATLLST
                     RPEGCDDVLVDAGHVTEVHERALAVLIAASEWARQTGGRLRFAAMSPALVDTAEYFDV
                     TDLLPRLPGPGGRAGGAAHLQAR"
     gene            2202..>3372
                     /locus_tag="VGD55_09150"
     CDS             2202..>3372
                     /locus_tag="VGD55_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0870554.1"
                     /translation="MADREIDPSFLDLPLRSLADAALSRARELGVTHADVRVERLRSQ
                     HLRLHDASLEGRLDDEDTGFAVRVVHEGTWGFAAAVDLTPEAAVRVAEQAVAIAKASR
                     VVNREPVELADEPVYPDKHWVSAYDVNPFDVPDTDKVALLAERSARLLAADGIDHVDA
                     AVAQVQENKFFADLSGTMTTQQRVRLNPTLAAVRIDADSGRFETMESLAPPAGRGWEF
                     LTGTGWDWDSEIAEIPELLAEKMKAPSVEAGNYDLVIDPSNLWLTIHESIGHATELDR
                     ALGYEAAYAGTSFATFDKLGTLAYGSPHMHVTGDRTAKYGLATIGYDDDGVAGQQWDL
                     IRDGVLVGYQLDRRMAKLKSLGRSNGCAFADSSGRVPLQRMANVSLQPTPNGPSTQ"
BASE COUNT          561 a         1188 c         1119 g          504 t
ORIGIN      
        1 tcgggtctcc cagatggcgg cgagctcgtc gaagccggga atccccttgg cggccatcgt
       61 gcggatcggc cccgcggaga ccaggttgac cctgatgttc tgcttgccca ggtcgcgggc
      121 caggtagcgc gaggtagatt cgagcgccgc cttggcgact cccatccagt catagagcgg
      181 ccaggccact ttcgcgtcga agtcgaggcc gacgaccgac ccgccgcccg gcatcatcgg
      241 cagcgccgcc atcgtgagcg ccttcaacga gaaggcggag acgtgcaggg cggtcgcgac
      301 gtcgtcccag ccggtgttga ggaagttgcc gccgagggca tcgccgggcg cgaacgcgat
      361 ggcgtgcacg acgccgtcga ggccgtcgag atgcgcgcca acccgctcgg ggagagccgc
      421 caggtcgtcg acgttcgtga cgtcgagctc gatgaccgga ggtggctcgg gcagccggcc
      481 cgcgatccgc tcggtgagcc tgaacacccg gccataggac gacaagacca cgtgtgcgcc
      541 ctcttgctgc gcaagcctcg cggcgtcgaa cgcgatcgat gcctctgtca ggacgccggt
      601 gatgagcagg cgcttacctg cgagtaggcc cattcagtgc cccattccca acccgccgtc
      661 gaccggaatg acggcaccgg tgatgtaggc ggcctcgtcg ctggccagga agcggatcac
      721 gccggcgatc tcgtcggtgg tcgcgtaacg cttcagcggg atctgatcga gcaactcggc
      781 ccgcctcggc tcgcgcagga ccgccgtgag gtcggtctcg acgaaccctg gcgcgaccac
      841 gttggccgtg atgccacgcg agccgaactc gcgtgccagc gaccgggcaa agccgaccag
      901 ccccgacttg gtcgccgcgt agttggcttg acctgccgac cccgacagcc cgaccaccga
      961 cgacacgaag atcatgcggc cgccgtggtt gcgcagcatc gcgcgggcgg cccggcgcgc
     1021 aacccggtag gcgccggtca ggttcgtcgc gatgacgtcg tcccagtcgt cgtcggtcat
     1081 gcggagcagg agcgtgtcgc gcgtgatgcc ggcgttagcg acgagcacct cgacagggcc
     1141 gtgttcggcc tcgacgagct cgaacgccga ctcgacgtcg tcgggcttgg tcacgtcgca
     1201 gtggatcgcg aaaaagccct gcggcgcctc actcgaacgg tacgtcacgg cgacgcgatc
     1261 gccctgcgca gcgaacgcgt gcgctgcggc gaggccaata ccgcggcttc cgccggtgac
     1321 gagcacagag cgcggcacgc aagtcccttc tcaagctccg gcacggttgc cggcaacgac
     1381 acaatcggtc gctcgccgag gctaccgggt aaccggggac gggagcgcgc ccctgccccg
     1441 ccgatcgctt cgaggggtcc gtttacgcgg ggaaggcgca cccggccacg ctcagctacg
     1501 ccgggtggac taacgcaggt cgccgcaacg gtgggggcgc gatcgtccag cggcgcagga
     1561 atttcgctcc gtccggtcct gcgaccaggg ggtggcgatc ccgatcaaat aactgtcgtc
     1621 cagctacttg ggagaaccac atgagcacca tgaccgccga caccctcgga accgtgagcg
     1681 ccaacgtcct tgccgacggg atcatcgtcc gcctgaacgg ctcgctcgac gtggcttcgc
     1741 tgcctgacct gcgcgcgacg ctgctgagca cccgccccga gggctgcgac gacgtgcttg
     1801 tcgacgccgg ccacgtcacc gaggtgcacg agcgtgccct cgcggtcctc atcgccgcct
     1861 ccgagtgggc gcgtcagacc ggtggtcgcc tgcgcttcgc ggcaatgtcc cccgccttgg
     1921 tcgacaccgc cgagtacttc gacgtcactg acctgctgcc tcgtctgcct ggtccggggg
     1981 gacgggcagg cggggcggca cacctccaag ctcgctaaag ccgccgcgtt acccctgctt
     2041 ttcctggctc taccagcccg tcgcgcacac cgccccacct accgccgtcc cgccggaccc
     2101 ccttcccggc tggacggcgg taggcatgtc tgggcccggt cgtagggtgg gccttcgatc
     2161 actcgcctgt ggcggcgcgt agacacggag gtaaccggct gatggccgat cgcgagatcg
     2221 atccgagctt cctcgacctg ccgctgcgaa gcctggctga cgcagcgctg tcacgtgcgc
     2281 gagagctcgg ggtgacccac gccgacgtac gggtcgagcg ccttcgctcg cagcacctgc
     2341 gccttcacga cgccagcctc gaagggcgac tcgatgacga agacactggg ttcgccgtgc
     2401 gggtcgtgca cgagggcacg tggggattcg ccgcagcggt cgatctcacg cccgaagcgg
     2461 cggtccgggt cgccgaacag gcggtcgcga tcgcaaaggc gagcagggtg gtcaaccgcg
     2521 agccggtcga gctggccgac gaaccggtgt accccgacaa gcactgggta tcggcctacg
     2581 acgtgaaccc gttcgacgtc ccggataccg acaaggtggc gctgctcgcc gagcggtccg
     2641 cccgactgct cgctgcagac ggcatcgacc acgtcgatgc cgccgtcgcc caggttcagg
     2701 agaacaagtt cttcgctgac ctctcgggca cgatgacgac gcagcaacgg gtgcgcctga
     2761 accccacact cgcggccgtg cgcatcgatg ccgattcggg ccgcttcgag acgatggaaa
     2821 gcctcgcgcc gccagccggt cgtggttggg agttcctcac cggtaccgga tgggactggg
     2881 actccgagat cgccgaaata cctgagctac tcgccgagaa gatgaaggcc ccctcggtcg
     2941 aagccggcaa ctacgacctc gtcatcgacc cgtcgaacct gtggctgacc atccacgaat
     3001 cgatcggcca cgccaccgaa ctcgacaggg cgctcggcta cgaggcggcc tacgccggca
     3061 cgagtttcgc aaccttcgac aagctcggca ccctcgccta tggatcccca cacatgcacg
     3121 tcaccggcga ccgcaccgcg aaatacggcc tcgcgaccat cggctacgac gacgacggcg
     3181 tagccggaca gcaatgggat ctgatccgcg acggcgtgct cgtcggctac cagctcgacc
     3241 gccgcatggc caagctcaaa agccttggcc gatccaacgg ctgcgcgttc gccgactcaa
     3301 gcggtcgggt gccgttgcag cgcatggcga atgtgtcgtt gcagccaacg cctaacggcc
     3361 cgtcgacgca ag
//
LOCUS       DASWVF010000516         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_345134, whole genome shotgun sequence.
ACCESSION   DASWVF010000516 DASWVF010000000
VERSION     DASWVF010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345134"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..380)
                     /locus_tag="VGD55_09155"
     CDS             complement(252..380)
                     /locus_tag="VGD55_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870555.1"
                     /translation="MQGPRFLVARSAFVRARQAGLESTDRRLDIAKVRKGKPANPL"
     gene            complement(501..1775)
                     /gene="glyA"
                     /locus_tag="VGD55_09160"
     CDS             complement(501..1775)
                     /gene="glyA"
                     /locus_tag="VGD55_09160"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0870556.1"
                     /translation="MPDHLAALDATDPEIARLIRAEETLQAEKVRLIASENYVSQAVL
                     EATGTVLTNKYSEGYPGKRYYEGQQVIDQVEVLAINRAKELFGVDHANVQPYSGSPAN
                     LAVYLALAEVGDTVLGMALPMGGHLTHGWSVSATGKWFNAVQYNVGRESGRVDMDEVR
                     EIALRERPKIIFCGGTAIPRTIDFPAFAEIAREVDAVLVADIAHIAGLIAGGAHPSPV
                     GHAGVVSTTTHKTLRGPRGAMLMCDEEYAKAIDKAVFPGLQGGPHDHTTAAIAVALHE
                     AQQPSFREYAAAIVTNAKALAEELMARGFDLVSGGTDNHLILVDLTPQGVAGKPVAKA
                     LDRAGLVANYNTVPYDTRKPFDPSGIRLGTPAVTSRGMGVAEMRQIGAWIDEVVAAVA
                     KDDEDAVNATTDRVEAQVRELTSDFPAPGLPV"
     regulatory      complement(1787..1871)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            2047..>3343
                     /locus_tag="VGD55_09165"
     CDS             2047..>3343
                     /locus_tag="VGD55_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870557.1"
                     /translation="MSDVTTPQLSTRARTRVGRYARFVAAALIAVPISFFAAGVANAA
                     AATHFSVSAPANAVVGNAVTVTVTALDGSNAVATTYAGTVHFTSTDAGAVLPANSTFS
                     GGIGSFSVTFSAAGTQHVTVTDTSSSSITGTSGAVTVAQTTTHLSATAAATAPAGNSF
                     VVTVKALDGGGNVVTGYAGTVKLTSTDSAAVLPANSTLSSGQHAFTVTLKTAGSQTVT
                     ATDTANSGISGTTTTITIAPATTTHLLVTAPSSSVAGLSFVVAVTAKDAYQNTVTTYG
                     GTVHLTSTDTSATLGANAKLTNGVGNFTATLRTLGGRTVKATDTVTSSITGTSGTITV
                     VARTAPIRAAGSDRIGTAVAVSQLNFPAGGAGAVVLARSDDFPDALVAAPLAATTRAP
                     LLFVQGASLPTVTRVEISRVLGAHGTVYLIGSTSAIPASI"
BASE COUNT          551 a         1130 c         1099 g          563 t
ORIGIN      
        1 tcgccttcgg caagagccag ctcggtcggc ttgccgcccg gtcggggcga ttcgacgaag
       61 ccctcgagct gctgcgctcg gcccgagacg actacgtgcg tgatggcgag cacgccgaag
      121 tgctcgagac cgacgcgcga atcgcagagt gcctggtgct tcaaggagca ttcgccgacg
      181 cgcgcgcagc cgctgacgac gcgttggtgc gggcgtcgtc gaactcggcc atcttgcccc
      241 agattccctt gctacaaagg gttcgcgggc ttgcccttgc gaaccttggc gatgtcgagg
      301 cggcggtcgg tgctctcgag gccagcctgt cgggcgcgca cgaacgccga gcgcgccacg
      361 aggaagcgtg gaccttgcat gcgctggtga cgctacgccg aaccgttgac ctcgactcgg
      421 tcgacccgat gatcgagcag cggcaagccg cgctgttcga acaactcggc atcgtcgatg
      481 ttgccgagcc gcctgacggt ctagaccggc aggccaggcg ccgggaagtc ggaggtgagc
      541 tcacgcacct gtgcctcgac ccggtcggtg gtcgcgttga cggcgtcttc gtcgtccttc
      601 gcaacagcgg cgacgacttc gtcgatccac gcgccgatct gccgcatttc ggcgacaccc
      661 atgccgcggc ttgtcaccgc cggcgtgccg aggcgaatgc cagaggggtc gaagggcttg
      721 cgggtgtcgt agggcaccgt gttgtagttg gcgacgagtc cggcgcggtc gagggctttc
      781 gcgactggct tgccagccac gccctgcgga gtgagatcga cgaggatcag gtggttgtcg
      841 gtgccgcctg acacgagatc gaatccgcgc gccattagtt cctcggcgag tgccttcgcg
      901 ttcgtcacga tcgccgcggc gtactcgcgg aacgacggtt gctgcgcctc gtgcagagcg
      961 acggcgatcg cggccgtcgt gtgatcgtgc ggaccgccct gcaagccagg gaaaacggcc
     1021 ttgtcgatgg ccttcgcgta ctcctcgtcg cacatcagca tcgcgccgcg cgggccgcgc
     1081 agcgtcttgt gagtggtagt cgagacgacg ccggcatggc cgaccggcga cgggtgcgcg
     1141 ccacccgcga tcaggccggc gatgtgtgcg atgtcggcca cgagcacggc atcgacttca
     1201 cgtgcgatct cggcaaaggc aggaaagtca atcgtgcgag ggatcgcggt gccgccacag
     1261 aagatgattt tcggtcgctc gcgaagcgcg atctcgcgca cttcgtccat gtcgacgcga
     1321 ccgctctcgc ggccgacgtt gtactgaact gcgttgaacc acttgccggt cgccgacacc
     1381 gaccacccgt gcgtgagatg gccgcccatc ggcagcgcca tgccgagcac ggtgtcaccg
     1441 acctcggcga gcgcgagata caccgcaagg ttcgccggcg accccgaata aggctgcacg
     1501 ttcgcgtggt cgacaccgaa gagctctttg gcgcggttga tcgccagcac ctcgacctga
     1561 tcgatgacct gctggccttc gtaatagcgc ttccccgggt aaccctcgga gtacttgttc
     1621 gtcagcaccg tgccggtcgc ctcgagcaca gcctgcgaga cgtagttctc agaagcgatc
     1681 aggcgcacct tctcagcctg aagcgtctct tcggcgcgga tcagccgggc gatttcagga
     1741 tcggttgcgt caagtgcggc gaggtggtca ggcatggcgt ctcctcggtg gacacccagg
     1801 cgcacggcgt ccgcgtcgat cgctccccgg tggttgaccc caccatctat gcgccagtcg
     1861 cgacgccccg atcgtagtgc aaagggccga atgcagcggg acgccgaggg gccggcctcg
     1921 gcccggaaac gtgggcgctt ccgtccgagg caaacctctg ccaccatgac gcaaccaatc
     1981 cggctaggtg tgcgtctaac ggcgaactcg agccagtctt ggcagccatc actacgcggg
     2041 ggaccgatga gcgacgtcac cacaccacag ctgtccacac gagccaggac gcgagttggc
     2101 cggtacgcgc gcttcgtcgc ggcggcactg atcgccgtcc cgatctcatt cttcgcggca
     2161 ggcgtcgcga acgcagccgc cgccacccac ttctcggtga gtgcgcccgc gaacgcggtc
     2221 gtcggcaacg cggtgactgt gacggtgacc gccctcgatg gcagtaacgc cgtcgcgacg
     2281 acctacgcgg gcacggtgca tttcacgtca accgacgcag gtgccgtact cccggcgaac
     2341 tcgacgtttt ccggcggaat cggcagcttt agcgtcacgt tttccgctgc cgggacccag
     2401 cacgtgaccg tcactgacac cagttcatcg tcgatcaccg gcacctcggg tgcggtgacc
     2461 gtcgcgcaga ccacaacgca tctgagcgcg accgcagcgg cgaccgctcc ggcgggcaac
     2521 tcctttgtcg tgaccgtgaa ggccctcgac ggcggcggga acgtcgtgac cggctacgcc
     2581 ggaacggtca agttgaccag caccgattcg gcggccgtgc tgcccgcgaa ctcaacattg
     2641 tcgagcggcc agcacgcgtt cacggtgacg ctcaagacgg ccggcagcca gacggtcacc
     2701 gcaactgaca cagcgaactc ggggatctca gggacaacga ccacgatcac catcgccccc
     2761 gctacgacga cgcacttgct ggtcactgcg ccgagctcgt cggtcgccgg tctgtcgttc
     2821 gtggtcgcgg tgaccgcgaa ggacgcctac cagaacaccg tcacgaccta tggcggcact
     2881 gtccacctca cgtcgaccga cacgtcggcg acactcggcg cgaatgccaa actcaccaat
     2941 ggcgtcggga atttcacggc aaccttgcgc acgttgggcg gccgcaccgt gaaggcgacc
     3001 gacaccgtga cctcgtcgat caccggaacg tcgggcacca tcacggtcgt tgcgcgcacg
     3061 gcgccgattc gcgctgctgg atcggaccgc atcggtaccg cagtggcggt gtcgcagctg
     3121 aacttcccgg ccggcggcgc cggcgcggtc gtgctcgccc ggtctgatga cttccccgat
     3181 gctttggtcg cggctccttt ggccgcgact accagggccc cgctgctgtt tgtgcaaggt
     3241 gcgtcattgc cgaccgtgac cagggtcgag atcagccgcg tgctcggcgc gcacgggacg
     3301 gtgtatctga tcggtagcac gtccgcgatt ccggcgagca tcg
//
LOCUS       DASWVF010000517         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_166609, whole genome shotgun sequence.
ACCESSION   DASWVF010000517 DASWVF010000000
VERSION     DASWVF010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166609"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGD55_09170"
     CDS             complement(<1..765)
                     /locus_tag="VGD55_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEY0870558.1"
                     /translation="MTEHAIEVWPGTPYPLGATYDGAGTNFAVFSEVAESVDLCLFSD
                     DDVETVITLPEYDAFVWHGYLPSISPGQRYGYRVHGPYEPSQGARCNPKKLLLDPYAK
                     AVQGNVDWDESLFGYPFGHPDERNDADSAAHAMKAVVINPYFDWRSDHQLRVPYHESI
                     IYEAHVKGMTIEHPDIPESIRGTYAGLAHPVMVDYLNSLGITAVELMPVHQFVTDSGL
                     VDKGLSNYWGYNTIGFFAPHNGYSAAGDRGEQVQEFK"
     gene            1081..>1872
                     /locus_tag="VGD55_09175"
     CDS             1081..>1872
                     /locus_tag="VGD55_09175"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0870559.1"
                     /translation="MRSRDGLMTVVAAIVGVLVFSSLTACERSVAIDTLDTVPSAPVS
                     SAPPVSSAKPTPAPPAQVTIAPADGASAVAPDARVSVSVANGTLTDVTVHDPTGDTVV
                     GELGQNAHSWDSTGSLAPATQYLVTATAVDATGTATERSTSFTTAAPANVLGVKIAPL
                     EGEVVGVGMPIIVYFTAAVTDEAAVERALTVEASEPVVGSWHWYGNKEVHYRPMDYWP
                     AGDQVTLHVNLKGVDAGKGVWGTADRTLNFTIGDSHVSTVNALTHK"
BASE COUNT          321 a          609 c          617 g          325 t
ORIGIN      
        1 cttgaactcc tgcacctgct cgccgcggtc gccggctgct gagtagccgt tgtgcggggc
       61 gaagaaaccg atcgtgttgt atccccagta gttcgacaat cccttgtcga cgaggccact
      121 gtcggtgacg aactggtgca ccggcatcaa ctccacagcc gtgatgccga gcgagttcag
      181 gtagtcgacc atgaccgggt gcgccagccc ggcgtaggtg ccgcggatgc tctcggggat
      241 gtcgggatgc tcgatcgtca tgcccttcac gtgggcctcg tagatgatcg attcgtggta
      301 cggcactcgc aactggtggt cgctgcgcca gtcgaagtac gggttgatga ccactgcctt
      361 catcgcgtgc gccgccgaat cagcgtcgtt gcgctcgtcg gggtggccga acgggtagcc
      421 gaagagggac tcgtcccagt cgacgttgcc ctgcaccgcc ttcgcgtacg ggtcgagcag
      481 cagcttcttc gggttgcacc gggcgccctg cgagggctcg taggggccgt gcacccggta
      541 gccatatcgc tggcctgggc tgatgctggg gaggtaaccg tgccagacga acgcgtcgta
      601 ttccggcagc gtgatgacgg tctcgacgtc gtcgtcgctg aacaggcaca ggtcgaccga
      661 ttcggcgacc tcggagaaca ccgcaaagtt ggtgccggcg ccatcgtagg tcgcgcctag
      721 cgggtagggc gtgcctggcc agacttcgat cgcatgctcg gtcatgtcat cccgtcgtcg
      781 tccggcggct cgtcaactct gacattgccc caacactgtg cggcaaacct gttccaaact
      841 tatggcagca gccagtgaac gaaggggggg cctagggcgt catgctggtg gcacccccgt
      901 agccgcaatc agctgcaacc agccgtaacc caatcgagtc ctacagggaa cggatggcgg
      961 tgccgagagg aacggcacga caactagcac gaatacccgc caaatccccc cgaccggggg
     1021 gaccttgacg cacactggaa gttcgtccgc acatttggca gggtcttcga aggaggcacg
     1081 gtgcgttcgc gggacggcct catgacggtg gtcgcggcca tcgtcggcgt cctggtgttc
     1141 tcgtcgctga ccgcgtgcga acgctcggtc gccatcgaca ccctcgacac cgtgccctcg
     1201 gctccggttt cgtcagcgcc gcccgtgtcg tcggcaaagc caacgcctgc gcctcccgcg
     1261 caggtgacga tcgcgccggc cgacggtgcc agtgcggttg cccccgacgc ccgggtctca
     1321 gtgtcggtcg cgaacggcac cctgacggac gtcacggtgc acgacccgac cggtgacacg
     1381 gttgtcggcg aactcggcca gaacgcgcat tcgtgggaca gcaccggctc gcttgcgcca
     1441 gccacgcagt acctcgtgac cgccactgcc gtcgatgcga ccggtacggc gaccgagcgg
     1501 tcgacctcgt tcacgacagc cgcgccggcg aacgtcctcg gcgtgaagat cgcgccgctc
     1561 gaaggcgagg tggtcggggt cgggatgccg atcatcgtct acttcaccgc cgcggtcacc
     1621 gacgaagccg cggtcgagcg agcactaacg gtcgaggcga gcgagcccgt cgtcggttca
     1681 tggcattggt atggcaacaa ggaagtgcac taccggccga tggactactg gcccgccggc
     1741 gaccaggtga ccttgcacgt caacctcaag ggcgtcgacg ccggtaaggg tgtgtggggc
     1801 accgctgacc gcacgctgaa cttcacgatc ggagattcgc atgtctccac cgtcaacgcg
     1861 ttgacgcaca ag
//
LOCUS       DASWVF010000518         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_90419, whole genome shotgun sequence.
ACCESSION   DASWVF010000518 DASWVF010000000
VERSION     DASWVF010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90419"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VGD55_09180"
     CDS             <1..973
                     /locus_tag="VGD55_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY0870560.1"
                     /translation="HGSLQPVDDAPELSPPVIQTLMYAILTQRQRERFEAELELDFAY
                     AVPGQSRFRVNMYRQRDAVGAAFRLIPFDIKPLEALGIPPSVSNFAMLPRGFVLVTGP
                     TGSGKSTTLAGIVDLANRKRRDHIMTVEDPIEFLHQHKSCLINQREVGEDTHSFTNAL
                     KHVLRQDPDIILVGEMRDLETISVALTAAETGHLVFATLHTQDAAQTIDRVIDVFPPY
                     QQQQVRVQLAGALQGVVSQTLCKTADGRGRQVATEVLVATPAIRNLIREGKTHQIYSA
                     LQAGARHGMHTMDQHLAELVKGGRITYETALEKCNNVEDFNRLSGRG"
     gene            987..>1682
                     /locus_tag="VGD55_09185"
     CDS             987..>1682
                     /locus_tag="VGD55_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0870561.1"
                     /translation="MTTTFQYTVRDRAGKLVKGTISAENQTMVMQRLKAGGYAPVSIT
                     QANSGMKKEITIPGFGKKVKLKDLSVMARQFATMVNSGLSLLRTLSILTEQTTNKELA
                     RVLGVVRNEVETGTALSAALAKHPRVFPPIMINMVRAGEVGGFLDSVMMQLAVNFESE
                     VKLRGKVKSAMTYPVVVLCISVVAVIGMLLFIVPVFKKLFIQLGGKLPLPTQILVDLS
                     GMLEFLLPAAIIGA"
BASE COUNT          321 a          548 c          506 g          307 t
ORIGIN      
        1 gcacggcagc ctgcagccgg tcgacgacgc gcccgagttg tcgccgccgg tgatccagac
       61 gctgatgtac gcgattttga ctcagcgcca gcgcgaacgg ttcgaagccg agctggagct
      121 tgacttcgcc tatgccgtgc ctgggcagtc ccgcttccgc gtcaacatgt accgccagcg
      181 cgacgcagtc ggtgccgcct tccgcttgat tcccttcgac atcaagccgc ttgaggcact
      241 gggcatcccg ccgtcggtat cgaacttcgc catgttgcct cgtggcttcg tgctggtcac
      301 cggtccgacc gggtctggca agtcgaccac cctcgccggc atcgtcgacc tcgccaaccg
      361 caagcgccgc gaccacatca tgacggtcga agacccgatc gagtttctgc accagcacaa
      421 gtcttgtctc atcaaccagc gagaagtcgg cgaggacacc cactcgttca cgaacgcgct
      481 gaagcacgtt ctgcggcaag accccgacat catcttggta ggtgagatgc gtgacctcga
      541 gacgatctca gtggccctga cggctgctga gaccggtcac ttggtcttcg cgaccctgca
      601 cacgcaggac gccgcacaga cgatcgaccg tgtcatcgac gtcttcccgc cttaccagca
      661 gcagcaggtt cgggtgcaac tcgccggtgc tcttcagggt gttgtcagcc agacactgtg
      721 caagaccgcc gatggccgcg gccgccaggt cgccacagag gtgttggtgg caacccccgc
      781 gatcaggaac ctgatccgcg agggcaagac gcaccagatc tactcggcgc tgcaagccgg
      841 tgcgcgtcac ggcatgcaca cgatggacca gcacctggca gaacttgtca aggggggccg
      901 gatcacctac gagaccgcac tcgagaagtg caacaacgtc gaagacttca accggctttc
      961 cggccgggga tgacggggat cacgacatga ccacgacgtt ccagtacacg gtccgagatc
     1021 gcgcgggcaa actcgtcaag ggcacgatca gcgctgagaa ccagacgatg gtcatgcagc
     1081 gcctgaaggc tggcggctac gcgcccgtca gcatcaccca ggcgaattcc gggatgaaga
     1141 aagagatcac catcccgggg ttcggcaaga aggtgaagct caaggacctt tcggtcatgg
     1201 cccggcagtt tgccacgatg gtcaactccg gtctctcgtt gctgcgtacc ctgtcgatcc
     1261 tgaccgagca gaccaccaac aaagagctgg ctcgggtact cggcgtcgtg cgcaatgagg
     1321 tcgagacggg taccgctttg tcggccgctc tcgccaaaca cccacgtgtg ttccccccga
     1381 tcatgatcaa catggtgcgg gccggcgagg tcggcggatt cctcgactcc gtcatgatgc
     1441 agctcgcggt gaacttcgag tccgaggtca agttgcgcgg caaggtgaag tcggccatga
     1501 cctacccggt cgtggtgctt tgcatctccg tcgtcgccgt catcggcatg ctgctgttca
     1561 tcgtgccggt gttcaagaag ctgttcatcc agctcggcgg caagctgcca ctgccgaccc
     1621 agatcctcgt ggacctgtcc ggcatgctcg agtttctgct gccggcggcg atcattgggg
     1681 ca
//
LOCUS       DASWVF010000519         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_218104, whole genome shotgun sequence.
ACCESSION   DASWVF010000519 DASWVF010000000
VERSION     DASWVF010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218104"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VGD55_09190"
     CDS             complement(<1..422)
                     /locus_tag="VGD55_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0870562.1"
                     /translation="MPQGLNWAIGGLSDGGFCAANLALRHPGSFGAVASLDGFYSAYS
                     DLAVMNKVFGVGSPAIAANDPSTLAVDVQSELPRFWIMSGSGAASDTFAAQSFRQIVT
                     TREPIEWVVLRGGQHTPPAWRATLPSLLTWTWLAISGGP"
     gene            complement(407..1228)
                     /locus_tag="VGD55_09195"
     CDS             complement(407..1228)
                     /locus_tag="VGD55_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870563.1"
                     /translation="MFGPTSWVFLGALALGFAGLLWWLARAGHLALKVVAGVLAFALS
                     TLFGAALVNQHYAYYTSWGSMVAAATGSRVVGYEAGFGLGGTSAGSNATDLLGRARMN
                     GSRGQRSATTQQTVTNEQSPIRFPIATKGLPGGVSIPTISVRAAATAGTGRVVELALP
                     GARSGINRKGFVYLPPQYFDPAYAHTYFPVVELLHGDPGDPSGWIYALQVPEIMDRGI
                     NTGSIGPMIVVMPATFSGQHGEDCVNAPMGSRTTPMSASTFPQTSSATSGPCRKA"
     gene            complement(1392..>1602)
                     /locus_tag="VGD55_09200"
     CDS             complement(1392..>1602)
                     /locus_tag="VGD55_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870564.1"
                     /translation="AKHHVATGVNASSHTFVYAADRAFVAAALLLVIAVALVASMRSG
                     QPRVMSGEPETAAELDADLEALGVA"
BASE COUNT          287 a          551 c          525 g          239 t
ORIGIN      
        1 gggcccccag aaatcgcgag ccaggtccag gtgagcagcg agggcagcgt cgcccgccag
       61 gccggcggag tgtgctgacc accccgcagc acgacccact cgatcggctc cctggtcgtc
      121 acgatctgcc ggaaggactg cgccgcgaag gtgtcgctcg cggcgcccga tcccgacatg
      181 atccaaaacc gcggcaactc tgattggacg tcgacggcga gcgtcgaggg gtcgttcgcc
      241 gcgatggcgg gcgatcccac tccgaagacc ttgttcatga cggcgaggtc ggagtacgcc
      301 gaatagaacc cgtcgagcga cgcgaccgct ccgaagctgc ccgggtgccg caacgccagg
      361 ttcgccgcgc aaaagccacc gtcggacaac ccgccgattg cccagttcag gccttgcggc
      421 atggccctga agtcgctgac gacgtctgcg ggaacgtcga cgctgacata ggtgtcgtcc
      481 tggagcccat gggggcgttg acgcagtcct cgccgtgctg gcccgaaaac gtcgcgggca
      541 tcaccacgat catcgggccg atcgagcctg tgttaatccc ccgatccatg atctcgggca
      601 cctgcagtgc atagatccag cccgacgggt cgccgggatc gccgtgcagg agttcgacca
      661 ccgggaagta ggtgtgggcg tacgcgggat cgaagtactg gggtggcagg tagacgaacc
      721 ccttgcggtt gatgccgctt cgcgcaccag gcagtgccag ctcgaccacg cggccggtgc
      781 cagccgtcgc ggctgcgcgc acgctgatgg tggggatgct gaccccgccg gggagacctt
      841 tagtcgcgat cgggaagcgg atgggcgact gctcgttcgt gaccgtctgc tgggtcgttg
      901 ccgatcgctg gcctctggaa ccgttcattc gtgcccgccc gagcaagtcg gtcgcgtttg
      961 agcctgcgga agtgccaccg agcccgaacc ccgcctcgta gccgaccacc cggctgccgg
     1021 tcgcggcggc caccatcgat ccccagctcg tgtagtaggc gtagtgctga ttcacaagtg
     1081 ccgcgccgaa cagggtgctg agcgcgaacg ccagcacgcc ggcgacgacc ttcagcgcca
     1141 ggtggccggc acgcgcgagc caccacagca ggccggcgaa accgagcgcc agtgctccca
     1201 agaagaccca gctcgtcggc ccgaacacgt tcacactcct tgtcgcctac gcactagacg
     1261 gccaagctgc cctcgaagtt gcaaccggtg ccaggttcca acgtccaaag cagtccccac
     1321 aaaaccagtc acaaagcaat gaggtcgagc ggccggtaga ccggccgctc gacctcgaga
     1381 tcgtgcgggt gttacgcgac gcccagtgcc tcgaggtcgg cgtcgagttc cgccgccgtt
     1441 tcgggctcgc cggacatcac ccgcggctgc cccgaccgca tgctcgcgac gagagccact
     1501 gcgatcacca ggagcagggc cgctgccacg aacgcgcggt cagccgcata cacgaaggtg
     1561 tgcgaactcg cgttcacgcc agtagcaacg tggtgcttgg cc
//
LOCUS       DASWVF010000520         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_218149, whole genome shotgun sequence.
ACCESSION   DASWVF010000520 DASWVF010000000
VERSION     DASWVF010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218149"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..422
                     /locus_tag="VGD55_09205"
     CDS             15..422
                     /locus_tag="VGD55_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870565.1"
                     /translation="MGAADPFQLFRILFEVRLGAGFAPETQIAFMLDQCHNIEPKIPG
                     QIRSVMNVQEATAKALLIDVDQLTAMQAAGDVLGANAVLMDAYNTDVRSLLADLREQM
                     GLDPNPMAAYAASGYAEKIAADRVGGQAAGWGA"
     gene            422..2470
                     /locus_tag="VGD55_09210"
     CDS             422..2470
                     /locus_tag="VGD55_09210"
                     /inference="COORDINATES: protein motif:HMM:NF006188.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEY0870566.1"
                     /translation="MALGDGAAQVAALLARSNRLGSDPTVTNYAGGNTSCKGNELDPV
                     TGQPVELLWVKGSGGDLGTLTEKGLAVLRLDRLRSMVGVYQGVEHEDDMVATFDFALH
                     GRGGAAPSIDTAMHGLVDADHVDHLHPDAGIAFATAADGAELTQRCFGDRVVWVPWRR
                     PGFQLGLEIAQAHRAHPNAIGVILGGHGITAWGSTSDECEARSMEIIRGAQAFIDAQG
                     KSEPFGAPRNSYTALPEAKRRERAAAIAPTVRGLASTDHVQIGHFDDDAEVLDFTASA
                     HCARLVELGTSCPDHFLRTKVRPMLLDLPTTASTDEILSRLRELHSSYRDEYRAYYER
                     HADADSPAMRGADPSIVLVPGVGMFSFGKDAQTARVAGEFYRNAIRVMRGAEAISTYA
                     PISEAEKFRIEYWQLEEAKLKRLPAPRPLAGRVALVTGAGSGIGRAIATRLCAEGACV
                     VVADRDAASADLVAKELGGRDKAIAVFMDVTSEDDVASAFGEAGLAFGGVDLVVNNAG
                     LSISKPLSETTLDDWDLQHDVMARGSFLVSRAAAKVMRAQGHGGDIVYISSKNSVFAG
                     PNNVAYGAAKADQAHQVRLLAAELGEFGIRVNGINPDGVVRGSGIFAGGWGAERAAVY
                     GVREEELGAFYAKRTILKHEVLPEHVAAAVFALTGGDLAQTTGLHIPVDAGVAAAFLR
                     "
     gene            2470..3966
                     /locus_tag="VGD55_09215"
     CDS             2470..3966
                     /locus_tag="VGD55_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEY0870567.1"
                     /translation="MTLRPAEPTRPPGDGPLPLQAASTTYGLDRVANRRPRVGLVAGG
                     LGTYWPQFPDLLPQLQRSAARVSERMTELNCEVIDVGFISDAEEGGVAAEKLRVAGCD
                     LIVGFLTTYMTATMIVPIAQRSGAPVLLINLQPTEVMDHATFDTGQWLAYCGACPLPE
                     MANAFERCGVPFRSVSGYVEDPNAWERISGWIKAAGVRGTLRNARHGSMGHLYPGMLD
                     VSTDLTMVTAHLGGHMEVVEFDDLRVRVDAVSEAEVEARVALAREIFELDGSVDADDF
                     AWAAKVSVGLDRLVDDFSLDSIAYYHRGLEGEIHERLGAGMILGSSLLTARGIPVVGE
                     FELRTSLAMLIMDRLGAGGSFTEFQALNFRDGVVEMGHDGPAHLAISDQRPLLRGLGV
                     YHGKRGFGVSVEFDVKHGPVTAFGIGQRRDGSYVGVAAEGEVVGGPLLRVGNTTSRVD
                     FGCDPGVWTDAWSGSGVGHHWALGTGHRIAELRATAELLELDLVEVRT"
     gene            3963..>5320
                     /locus_tag="VGD55_09220"
     CDS             3963..>5320
                     /locus_tag="VGD55_09220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEY0870568.1"
                     /translation="MTVEDIVVAAVDLGATSGRVMLGRIGPGGISMEHIHRFPNEPIQ
                     LSTGLHWDLYGLFDEVLVGLRAAQQSLGAGESISSIGIDTWAVDYGLLDADAELLGPP
                     FCYRDSRTAKGVDRVHAKVSPEELYAINGLQFLPFNTLYQLAAEQETTRWDNARTMLL
                     LPDLLAYWLTGEVGAESTNASTTGLFDARTGDWALSLVDSLEIPRSMLPPLREPGAVI
                     GQLLPDIAAKTGLPATTTVVAVGSHDTASAVLAVPADGDRFAYISSGTWSLVGLELPA
                     PILSEASRTANFTNERGVDGTTRYLRNVAGLWLLNESIRSWNASGWQVDLHDLLEAAA
                     ALPDGGPVVDPNDAEFLAPGDMPARIDLACRESGQLAPTDPPAVVRCILDSLAHAYAD
                     TLADAVRLSGTSIETVHIVGGGSSNAFLCTLTARATGLPVVAGPVEATALGNVLVQAR
                     TAG"
BASE COUNT          882 a         1697 c         1792 g          949 t
ORIGIN      
        1 acgacgacct catcgtgggc gcggccgacc cgttccagct gttccggatc ctgttcgagg
       61 tccggctcgg cgccgggttc gcccccgaga cacagatcgc gttcatgctg gaccagtgcc
      121 acaacatcga gccgaagatc ccgggccaga tccgctccgt catgaacgtt caggaggcaa
      181 cagcgaaagc tctgctcatc gatgttgacc aactgaccgc gatgcaggcg gccggcgacg
      241 tgctgggtgc caatgccgtg ctgatggacg cgtacaacac cgacgtacga tcgttgttgg
      301 ctgacttgcg cgaacagatg ggactggacc ccaacccgat ggcggcatac gcggcatccg
      361 gctacgccga gaagatcgcg gccgatcgtg tcggcggcca ggctgccggc tggggtgcgt
      421 gatggcctta ggtgatgggg cggcgcaggt cgcggcgctg ctggcccgaa gcaaccggct
      481 cggatcggat ccgaccgtca cgaactacgc gggcggcaat acgtcgtgca agggcaacga
      541 actcgacccc gtcacgggcc aaccggtcga actgctgtgg gtcaagggtt caggtggtga
      601 ccttggaact ttgaccgaga aggggctcgc ggtcctgcgc ctcgatcgtc tgcggtcgat
      661 ggttggtgtc taccaaggcg tcgagcacga ggacgacatg gtggcgacgt tcgacttcgc
      721 cctgcacggt cggggcggcg cggcgccgtc gatcgacacc gcgatgcacg gcctggtcga
      781 cgccgaccac gtcgaccatc tgcaccccga cgcgggcatc gccttcgcca ccgcggcaga
      841 cggcgcggag ctgacgcaac gctgcttcgg cgatcgcgtc gtgtgggttc cctggcgccg
      901 gccagggttc cagctcgggc tcgagatcgc gcaagcgcat cgcgcgcacc ctaatgcgat
      961 cggggtgatt cttggcgggc acggcattac tgcctggggc tcgacaagtg acgagtgcga
     1021 ggcgcgctcg atggagatca tccgaggggc gcaagcattc atcgacgcgc aggggaagtc
     1081 cgagccgttc ggcgccccgc gcaatagcta caccgcgttg ccggaagcta aacggcgtga
     1141 gcgcgcagcc gccattgcac cgacggtacg tgggctggcg tcgaccgatc atgtgcagat
     1201 cggccacttc gacgacgacg ccgaggtgct cgacttcacg gccagcgcac actgtgcgcg
     1261 acttgtcgag ctcggcacct cgtgccctga tcacttcctg cgcaccaagg tgcgcccgat
     1321 gctcctcgac cttccgacaa ccgcaagcac cgacgaaatc ctgagccggt tgcgcgagtt
     1381 gcacagcagt taccgcgacg aataccgggc atattacgag cgccatgcgg acgccgattc
     1441 gccggcgatg cgtggagccg acccatccat cgtgctggtg ccaggcgtcg gcatgttcag
     1501 cttcggtaaa gacgcccaga cggctcgagt cgcgggggag ttctaccgca acgcgatccg
     1561 cgtcatgcgt ggcgcagagg cgatctcgac ctacgcgccg atctctgagg cggagaaatt
     1621 ccgtatcgag tactggcagt tggaggaagc gaagctcaag cggctgccgg caccgcggcc
     1681 gctcgcgggc cgggtcgcgc tggtgaccgg tgccgggtct gggatcggcc gggcgatcgc
     1741 aacccggcta tgtgccgaag gtgcctgcgt ggtcgtcgcc gatcgtgacg cggcgtcggc
     1801 cgacctggtc gccaaggaac ttggcgggcg cgacaaggcg atcgccgtgt tcatggacgt
     1861 gaccagtgaa gacgacgtgg cgtccgcgtt cggcgaggcc gggctggcct tcggcggggt
     1921 cgatctcgtc gtgaacaacg cggggctgtc aatctcgaag ccgttgagtg agacgacact
     1981 ggatgactgg gacctccagc acgacgtcat ggcccgcggt tctttccttg tctcacgggc
     2041 ggcggcgaag gtgatgcgcg cccaaggaca cggtggcgac atcgtctata tctcaagtaa
     2101 aaactcggtg ttcgccggac cgaacaacgt cgcgtacggc gcggcgaaag ccgaccaggc
     2161 gcaccaggtg aggctgctcg cggccgagct cggcgagttc ggcattcgcg tcaacggcat
     2221 caaccccgac ggagtcgtgc ggggttcagg catctttgct ggcggttggg gcgcagaacg
     2281 cgctgcagtc tatggagtgc gagaagagga gctgggggct ttctatgcca agcgcacgat
     2341 cctcaagcac gaggtgttgc ccgagcacgt cgcggccgcg gtgttcgcgt tgacgggcgg
     2401 cgacctcgcg cagacgaccg gcctgcacat ccccgtcgac gcgggcgtcg ctgccgcctt
     2461 cttgcgctga tgacgctccg tccggcggag ccgactcgtc cacccggtga tggcccgctg
     2521 cccttacaag cagccagcac gacgtacggt ctcgatcgcg tcgccaaccg gcgtccgcgc
     2581 gtcgggctcg tcgccggcgg gctcggcacc tactggccgc aattccccga tctcctgccg
     2641 caactgcaac gatctgccgc gcgcgtgtcg gagcggatga cggaactgaa ttgcgaggtc
     2701 atcgacgttg gcttcatctc cgacgccgaa gaaggtggcg ttgcggcgga gaagttgagg
     2761 gtggccggct gcgatctcat cgtcggcttt ctcacgacgt acatgacggc aacgatgatc
     2821 gtgccgatcg cccagcgcag tggcgcccct gtgttgctca tcaacctcca gccaacggag
     2881 gtgatggatc acgcaacctt cgacaccggt caatggctcg cgtactgcgg cgcgtgccca
     2941 ctccccgaga tggccaacgc gttcgagcgc tgcggcgtcc cgttcagatc agtgtcgggc
     3001 tacgtcgaag acccgaacgc gtgggagcgc atcagcggct ggatcaaggc agccggggtg
     3061 cgcggcacgc tgcgcaatgc gcggcacggt tcgatggggc acctttaccc gggaatgctc
     3121 gacgtatcga ccgacctgac gatggtgacg gcgcacctcg gcgggcatat ggaagtggtg
     3181 gagttcgacg acctgcgggt gcgcgtcgat gccgtgagtg aggccgaagt cgaagccaga
     3241 gtcgccctcg cccgcgagat cttcgaactc gacggaagtg tcgacgccga cgatttcgcc
     3301 tgggccgcaa aggtttctgt tgggctcgac cgccttgtcg acgacttctc cctcgactcg
     3361 atcgcctact accaccgtgg gctcgagggt gagattcacg agcgtctcgg tgccggcatg
     3421 atcctcggtt cgtcgttgct caccgcccgc gggatcccag tcgtgggcga gttcgaactg
     3481 cgcacgtcgc tggcgatgct catcatggat cgcctcggcg ccggcggatc cttcaccgag
     3541 ttccaggcac taaacttccg cgatggcgtc gtcgagatgg gccatgatgg gccggcccac
     3601 ctcgcgatca gcgaccagcg gccattgcta cgcggtctcg gcgtctacca cggcaagcgg
     3661 ggcttcggag tgtccgtcga gttcgacgtg aagcacgggc cggtcaccgc gttcggtatc
     3721 ggccaacgca gggacggtag ttatgtcggt gtcgctgccg agggtgaggt tgtcggcggc
     3781 cctttgctac gcgtcggcaa tacgacgtcc cgcgtcgatt tcggttgtga ccccggtgtc
     3841 tggactgacg cctggagcgg gtctggcgtc ggtcaccatt gggcgcttgg caccggccac
     3901 cggatcgccg aactgcgcgc gaccgcggag ttactcgaac tcgatctcgt cgaggtccgc
     3961 acttgacggt cgaagacatc gtcgttgccg cggtcgatct cggcgcgacc agtggccgcg
     4021 tgatgcttgg tcgcatcggc ccgggtggta tctcgatgga acacattcac cggtttccca
     4081 acgagccaat ccagctgtcg accgggttgc attgggatct gtacggcctt ttcgacgagg
     4141 ttctagtcgg ccttcgcgct gcacaacaat cactgggtgc tggcgaatcg atttcgagta
     4201 tcggtatcga cacgtgggcc gtcgattacg ggcttcttga cgcggacgcc gaattgctgg
     4261 gtccgccgtt ctgttaccga gactcacgta ccgcaaaagg cgtcgatcgg gtgcatgcca
     4321 aagtttcgcc cgaggagttg tacgcgataa acggcctgca gttcttgccg ttcaacacgc
     4381 tttaccaact tgcggccgag caagaaacca cgcgctggga taacgcgcgc acgatgctgc
     4441 tattgcccga cctgcttgcg tattggctca ccggtgaagt cggtgccgaa tcgacgaatg
     4501 cctcgacaac cgggcttttc gatgcgcgca cgggcgactg ggcgctctca ctggtcgaca
     4561 gtctcgagat tccgcgcagt atgttgccgc cgctgcgcga acctggtgcg gtaattggtc
     4621 aactgctgcc ggatatcgca gcgaagacgg ggctgccggc gacgacaacc gtggtcgcag
     4681 tcggctcgca cgacaccgca tctgccgtcc tcgcggtgcc ggcggacggc gaccggttcg
     4741 cctacatctc gtcgggcacc tggtcactcg tcgggctcga gctgccggct ccgatcctca
     4801 gcgaggcgag ccgcaccgca aacttcacga acgaacgcgg cgtcgacggc acgacgcgct
     4861 acctgcgcaa cgtcgccggt ctttggttgc tgaacgagtc gattcgttcg tggaatgcga
     4921 gcggatggca ggtcgacttg cacgacctgc tcgaagcggc ggccgcgctg cccgatggcg
     4981 ggccagtcgt cgaccctaat gacgcagagt tcctggcgcc cggcgatatg ccggctcgca
     5041 tcgacctggc atgtcgtgag tctggacagc ttgcgccgac cgacccaccg gccgtcgttc
     5101 gctgcatcct cgacagcctc gcgcacgcgt acgcggacac gctcgccgac gctgtgcggc
     5161 tttcgggtac gagcattgag actgttcaca tcgtcggtgg cggatcgagc aacgccttcc
     5221 tctgtaccct gaccgctcgc gcgacggggc tgccggtggt cgccgggccg gtcgaggcga
     5281 ccgccctcgg caacgtgttg gtgcaggccc ggactgccgg
//
LOCUS       DASWVF010000521         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_116020, whole genome shotgun sequence.
ACCESSION   DASWVF010000521 DASWVF010000000
VERSION     DASWVF010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116020"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VGD55_09225"
     CDS             <1..1021
                     /locus_tag="VGD55_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0870569.1"
                     /translation="SVAAAISPNDRLQQRFAPWTTYVIVPLFALANAGIPISGHFLSK
                     AVTSPITLGILVGYVLGKPIGIVGMSWLVTAITRRRLRPPVGWAAVAGAGTIAGIGFT
                     VSLLIASLAFTGTQLQQAKAGVLAAAVLASLVTWIIFRVTERLPKRQRIVALLGRSEL
                     IVDLVDEVDVERDHIRGPQESLVTLLEYGDLECPYCGQAEPVVRELLSEFGDIRYVWR
                     HLPLTDVHPNAQLAAEATEAAALQGAFWEMHDLLFTHQDALHMKDLIRYAGELGLDVE
                     RFTDDVKHHVGAARVASDVDSADLSAVSGTPTFFVNGVRHHGAFDITSLKTAVTAAGA
                     RAIIA"
     gene            1148..>2695
                     /locus_tag="VGD55_09230"
     CDS             1148..>2695
                     /locus_tag="VGD55_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0870570.1"
                     /translation="MQTSPRQRKSRLRRVGTATAVVGSALALSLPVAASAVQPTTPTV
                     STTTTPIKHLVVIFQENVSFDHYFGTYPSATNSSGQPFNAAHGTPKVNNLVDTKGLGG
                     VGNLLTNNPNKDAANAQVNPQRLDPATINGILTCDQDHDYNDEQKAFDGGAMDKFVTT
                     VGTASGKSGPGQPCSADDVMNYYDGNTVTGLWNYAQQYAMSDNYFAATYGPSAPGAIN
                     LISGDTGNVGLQINGAATDGDTVSDGNGGFSLIEDAQPYYDDCSTRDAASLTGTNIGD
                     ELNAAGLSWGWFQGGFKPTTSFAVATAASPQPTSTFTPDQFKGKFATAPASDQGLCNA
                     VHPVGAAVGGTGGTVAGPTNYGNKDDYIAHHEPFQYYASTANPHHLAPASLAAIGTDT
                     QTSIAGVPQFDTANHQYDTSDFNSLVGAISHGYLSADHLPAVSFLKAPGYQDGHAGYS
                     DPIDEQKFVVDEINALQHTPDWSSTAVIVTYDDSDGYYDHVFSGVHNASNTASVTTPP
                     GPQDFLSG"
BASE COUNT          518 a          931 c          814 g          432 t
ORIGIN      
        1 gagcgtcgcc gcagctatct cgccgaacga ccggcttcag caacgtttcg cgccctggac
       61 cacgtacgtc atcgtgccgc tgttcgcgct tgccaacgcg ggtattccga tcagcgggca
      121 cttcctgtcg aaggcagtca cctcaccgat cacgctcggg attctcgttg gatatgtgct
      181 cggtaagccg atcggcattg tcggcatgtc gtggctcgtc accgccatca cgcgccggcg
      241 gctgcgacct ccggtcggct gggcggcggt cgctggagcc ggcacgatcg cgggcatcgg
      301 cttcaccgtc tccctgctga tcgcctcgct cgccttcacc ggaacccaac tccagcaagc
      361 aaaagccggt gtgctggccg ccgcggtact cgcgtcgctg gtcacctgga tcatcttccg
      421 cgtcaccgag cgattgccga agcgtcagcg gatcgtggcg ctactcggca ggtcggaact
      481 catcgtcgac cttgtcgacg aggtcgacgt cgagcgcgat cacatccgcg gaccacagga
      541 gtcgctcgtc actctgctcg agtacggcga cctcgaatgt ccctactgcg gtcaagccga
      601 gccggttgtt cgcgagttgc tcagcgagtt cggcgacatc aggtacgtgt ggcggcacct
      661 gccactcacc gacgtgcacc cgaacgcgca gctcgcggcc gaggccaccg aagcggctgc
      721 gctccaaggt gcgttctggg agatgcacga cttgctgttc acgcatcaag acgcgctgca
      781 catgaaggac ctcatccggt acgcgggcga actgggactt gacgtcgaac gcttcaccga
      841 cgacgtcaag catcacgttg gtgcagcgag ggtcgcgagc gacgtcgaca gcgccgacct
      901 cagcgcggtg tccgggacgc cgacattctt cgtcaacggg gtccgccacc acggcgcctt
      961 cgacatcacg tctctcaaga ccgcggtgac ggctgccggg gcccgcgcga tcatcgcctg
     1021 accgctcggt ttggctttcg ttcacctaac gctcacccgc catccggccg gagtcactca
     1081 gccttttacc agcgtcgcgt caggtaggag gtggccaccg ccgtggtggt caagtgagga
     1141 gacagccatg cagaccagcc cgcgccagcg gaagtcgagg cttcgcagag tcgggacggc
     1201 gacagcagtc gtcggatcgg cccttgcgct cagccttccg gtggcagcta gcgccgtgca
     1261 gccgaccaca cccacagtga gcaccaccac tacgccgatc aagcacctcg tggtgatctt
     1321 ccaggagaac gtctcgttcg atcactactt cggcacgtat ccgagcgcga cgaacagctc
     1381 aggccagccg ttcaacgcag cgcacggaac gccgaaagtc aacaacctgg tcgacacgaa
     1441 agggctcggt ggcgtcggca acctgttgac caacaatccc aacaaggacg ccgcgaacgc
     1501 tcaggtgaac ccgcagcgac tcgatcccgc gaccatcaac ggcattttga cgtgtgacca
     1561 ggaccacgac tacaacgacg agcagaaggc gttcgacggc ggggcgatgg acaagttcgt
     1621 caccacggtc ggcaccgcat cgggcaagag cggccccggc cagccgtgta gcgccgacga
     1681 cgtcatgaac tactacgacg gcaacaccgt caccgggttg tggaactacg cgcagcaata
     1741 cgcgatgagc gacaactatt tcgccgcgac gtacggccct tcggcgccgg gcgcgatcaa
     1801 cctcatctcc ggcgacaccg gcaacgtggg cttgcagatc aacggtgcag ccactgacgg
     1861 cgacaccgtc agcgacggca acggcgggtt ctccctcatc gaggacgcgc agccttacta
     1921 cgacgactgc tccacgcggg acgccgcatc gctcacgggt acgaacatcg gcgacgaact
     1981 gaacgcggcc ggactcagct ggggctggtt ccagggtgga ttcaagccga ccacgtcgtt
     2041 cgcggtagcg accgctgctt ctccgcagcc gacaagcacg ttcacgcctg accagttcaa
     2101 gggcaaattc gcgacagcgc cggcgtccga ccaaggtctt tgcaacgccg tgcacccggt
     2161 cggggctgca gtcggtggca ccggcggaac ggtcgcgggg ccgacgaact acgggaacaa
     2221 ggacgactac atcgcccacc acgagccgtt ccagtactac gcgtcgacgg cgaacccgca
     2281 ccacctcgca ccggcgtcac tcgcggcgat cggcaccgac acgcagacga gcatcgcggg
     2341 cgtgccgcag ttcgacaccg cgaaccacca atacgacacg agtgacttca actcactcgt
     2401 cggcgcgatc tcacacggtt acctgtcagc cgaccacctg cctgccgtca gcttcttgaa
     2461 ggcgccgggc tatcaggacg ggcacgcggg ctactccgat ccgattgacg agcagaagtt
     2521 cgtggtcgac gagatcaacg cgttgcagca cacgcctgac tggtcgagca ccgccgtcat
     2581 cgtcacctac gacgactccg acggttacta cgatcacgtg ttcagcggcg tgcacaacgc
     2641 gtcgaacacc gcgtcggtca ccaccccgcc tggtccgcag gacttcctga gcggc
//
LOCUS       DASWVF010000522         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_319523, whole genome shotgun sequence.
ACCESSION   DASWVF010000522 DASWVF010000000
VERSION     DASWVF010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319523"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..492)
                     /locus_tag="VGD55_09235"
     CDS             complement(139..492)
                     /locus_tag="VGD55_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HEY0870571.1"
                     /translation="MQCFSTFAAAISAATDAVGPLTSVILSIDYTGTNESGSSLTWRQ
                     ASGCGSYSASSMPTGWNDAISSVSNFNGCGTTLYQNTSFSGSAVSVAVDATATTLGGF
                     NREASSQKWCAANSC"
     gene            complement(916..1413)
                     /locus_tag="VGD55_09240"
     CDS             complement(916..1413)
                     /locus_tag="VGD55_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870572.1"
                     /translation="MNFSRKTLIVGVTSATLLIGGGGVAMASTGGGSGATSSTAGLLT
                     SVSPDPTTTGGGITAIQQRVDARVVKLTTRLTKIQGRVDASTKLSAADKATLDADLSK
                     TVADLATAKQDVDAATTRADLRAARPALQTLRADVKQLVSDVKSIRAAASSGTPNATD
                     SALLT"
BASE COUNT          241 a          475 c          529 g          277 t
ORIGIN      
        1 gttgacacca cgagcggggc tccaccgggt agctcttcgc cgccctaagc ctgggaatgc
       61 ccgcaacggg gggcgggcat tcccacgaac aggtgcgggc cggtcgtctc atccgacgcc
      121 ggcccgcacc tgcactgttc agcaactgtt cgcggcgcac catttctgcg aggacgcctc
      181 gcggttgaag ccgccgagcg tggtcgctgt tgcgtcgacc gcgaccgaga cggctgaccc
      241 ggaaaagctg gtgttctggt aaagcgtcgt cccgcagccg ttgaagttac tcaccgatga
      301 gatcgcatca ttccaacctg tcggcatact gctcgcgctg taactgccgc atccactggc
      361 ttgcctccag gtgaggctgc tgcccgactc gttcgtaccg gtgtagtcga tactcagaat
      421 cacactcgtg agcggaccca cagcatcggt cgctgcagag atcgcggccg cgaacgtgct
      481 gaagcattgc attgcagggg gctgccccga gatagcagac ggttgcgcga cgcaatggcg
      541 cgcggcgggc tgggcagaag ctgagctggg aaggatcgcg agggagcacc ctagagtgct
      601 cgcgagaagg acggcggcta cgcgggcgtg tcgactgaac atctgatcct ccggtgtttg
      661 gccgtttccg ccggtgatcc gacggctcgg gcgcccgcac catcgcgggt cgcccggtac
      721 gcagagcact gatgcggcgg tgtaaataca cagcccggcc atccgccacg cagactcgcc
      781 cggagtagac cgcaacgtcc gattgcacta ggtgggcggc cgtggaagcg ttagttcaac
      841 ggtccggcag ctatcagcac gccggtgttg ccaccggcgt gctgacgtgc ggttctgggc
      901 cgctgtggag atcgcttagg tgagcagggc agagtcggtg gcgttgggcg taccactgct
      961 ggcggccgcg cgaatggact tgacgtcact gaccaactgc ttgacgtcgg ctcgcaacgt
     1021 ttgcaacgcc ggtcgcgcgg cccggaggtc ggcacgcgtg gtcgcggcgt cgacgtcctg
     1081 cttagcagtc gcgagatctg cgaccgtctt gctcaggtcg gcgtcgagcg tggccttgtc
     1141 ggctgcgctg agcttggtgc tcgcgtcgac cctgccctgg atcttcgtca ggcgcgtcgt
     1201 cagtttcacg acgcgggcat cgactcgctg ctggatcgcc gtgatgccgc cgcccgtcgt
     1261 ggtgggatcg ggcgacacgc tcgtgagcag gcccgccgtg cttgaggtgg caccagaacc
     1321 gccgccggtc gaggccatgg cgacgccgcc gccgccgatg agcaaggtgg cggatgtgac
     1381 gccgacgatg agggtcttgc gactgaagtt catggggaag ctcctggtcc gttgtccgca
     1441 actcgggccg agccgcgttt gtgtgttgcc tcaaatgtcg gcgggccacg tcaaccgcag
     1501 gcgcaggggt ttgtacggat ca
//
LOCUS       DASWVF010000523         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_192548, whole genome shotgun sequence.
ACCESSION   DASWVF010000523 DASWVF010000000
VERSION     DASWVF010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192548"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGD55_09245"
     CDS             complement(<1..342)
                     /locus_tag="VGD55_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0870573.1"
                     /translation="MDLGIYSFGDRHPDPFTGVQVSVADQYANTLERIKLADELGLSF
                     YGLGEHHLDTYAISNPGTVLAAAASVTKQITLASAVTVLSTEDPVRVYQQFTTLDQLS
                     RGRAELLAGRGS"
     gene            complement(395..793)
                     /locus_tag="VGD55_09250"
     CDS             complement(395..793)
                     /locus_tag="VGD55_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870574.1"
                     /translation="MTAGTAPALPLRAASWWYVGQMAAGLVGPVAVGDALADAVVGVA
                     AALDVAAAGDDAGALEASALDVPVLDAAAVDTAAADAAVLEADDFGWLDVHAASASMP
                     PTRTAISRRCPMPTSLPAFRPLTYPGCPGL"
     gene            804..>1898
                     /locus_tag="VGD55_09255"
     CDS             804..>1898
                     /locus_tag="VGD55_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0870575.1"
                     /translation="MTAKAKLDGAVYASPLVLRDSEGDLVIAASENNTLYGLRNNGSV
                     VWSRHIGTPVNGSTLPCGNINPTGITGTPVYDPATGLVFAVAFLSGDKHLLVAVNAET
                     GTLAWTRPVDAPGSHANVEQERGALLLSGGKVWIPYGGLNGDCGPYHGYLVGIATSGQ
                     GAAAIYQVPSSREAGIWTPAGPSADAAGHVYVSVGNSAQTNPNAAYDMGDSVIELSGT
                     TVVSFFAPATWATENARDLDLGTTGPVILPTGQVFVAGKNGNAYLMKQGALGGIGAKV
                     PSIAVCTAFGGAAYNDGTIYIPCTDGVRAVHISTSAMTGSWHASPSGSPIVGGGAVLT
                     VAPSAGTLFALDPASGTTKAQVNLGDSTTRF"
BASE COUNT          334 a          642 c          609 g          313 t
ORIGIN      
        1 cgagcctctg cctgcaagca attccgcgcg cccgcgactg agctggtcga gagtggtgaa
       61 ctgctggtag acccgcaccg gatcctcggt actcaacacc gtcacggccg aggccagcgt
      121 gatctgcttc gtgacgctgg ctgctgccgc cagcaccgtg cccggattgg agattgcgta
      181 ggtgtcgagg tggtgttccc cgagcccgta gaacgagagc ccgagttcgt cggcgagctt
      241 tattcgttcg agggtgttcg catactggtc ggcgactgat acctggacgc cggtaaaggg
      301 gtcggggtgt cgatcaccga agctgtagat gccgaggtcc atgccccttt gaacccgcgc
      361 tttcccgtcg ttgtttccga ctgatcagaa cacatcacaa cccagggcac cccgggtagg
      421 tcagtggccg aaatgcgggc agactggtcg gcatgggaca tcgtcgactg atcgccgtcc
      481 tcgtgggcgg catactcgcg ctcgcagcgt gcacgtccag ccacccgaag tcgtcggctt
      541 cgagcacggc agcgtcggcg gcggctgtat cgaccgctgc ggcatcaagt accgggacgt
      601 cgagtgcgct cgcttcgagc gcgcctgcat cgtcacctgc ggctgccaca tcaagcgcgg
      661 cggccacccc gaccaccgcg tcggccagcg catcgcccac cgccaccggt ccaaccaatc
      721 cggcagccat ctggccgacg taccaccacg acgcggcacg cagcggtaac gcgggcgctg
      781 tccccgccgt cacggcattg aaggtgaccg cgaaggcaaa gctcgacggc gccgtttacg
      841 ccagcccgct ggttttgcgt gacagcgagg gcgacctcgt catcgcagcg tcggagaaca
      901 acacgctgta cgggttgcgc aacaacggca gcgtcgtgtg gtcacgtcac atcggcacgc
      961 cggtcaacgg ttcgacgctg ccgtgcggca acatcaaccc gaccggcatc accggcacac
     1021 ccgtctacga tccggctact gggctcgtgt tcgcggtcgc gttcctgtct ggcgacaagc
     1081 acctgctcgt cgctgtcaac gcagagacgg gcacgctcgc atggacccgg cctgtcgatg
     1141 cgccgggctc ccacgcgaac gtcgagcaag aacgcggcgc cttgttgctc agcggcggca
     1201 aggtgtggat accgtacggc ggcctcaatg gcgactgcgg cccctaccac ggctacctcg
     1261 tcggtatcgc gacctcaggt cagggcgccg cggcgattta ccaagtgccg agctcgcgag
     1321 aggccggaat atggacaccg gccggcccgt cagccgacgc ggcaggacac gtctacgtgt
     1381 cagtcggcaa cagcgcgcag accaatccga atgccgccta cgacatgggc gactccgtca
     1441 tcgaactgag cgggacgacc gtcgtgtcgt tcttcgcgcc ggcgacatgg gccaccgaga
     1501 acgcgcgtga tctcgacctg ggcacgaccg gcccggttat tctgccgacc ggacaggtct
     1561 tcgtcgcagg caagaacggc aatgcctatc tcatgaagca aggtgcgctc ggcggcatcg
     1621 gtgccaaagt cccgagcatc gcggtgtgca cggcgttcgg aggcgcggcg tacaacgacg
     1681 gcacgatcta cattccttgc accgacggcg taagggcggt ccatatctcg acaagtgcga
     1741 tgaccggctc atggcatgca tcgccgtccg gctcaccaat cgtcggcggt ggcgccgtgc
     1801 tgacggtcgc gccgtcggcc ggcacgctgt ttgcactcga tccggcgtcc ggcacgacga
     1861 aggcgcaagt caacctcggc gactcgacca cgcgattc
//
LOCUS       DASWVF010000524         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_166758, whole genome shotgun sequence.
ACCESSION   DASWVF010000524 DASWVF010000000
VERSION     DASWVF010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166758"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGD55_09260"
     CDS             <1..181
                     /locus_tag="VGD55_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870576.1"
                     /translation="RVDVVLADQRVVAKADTLRSAAAELGGRLVLAPVAMSDGSPRHD
                     PELLAAAYARIFEID"
     gene            210..1190
                     /gene="whiA"
                     /locus_tag="VGD55_09265"
     CDS             210..1190
                     /gene="whiA"
                     /locus_tag="VGD55_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEY0870577.1"
                     /translation="MAMTSAVKDELSRLTVTKSCCRKAETSALLRFAGGLHLVGGHVV
                     VEAELDTGSSARRLRKDIAELYGHGSDIAVVAPGGLRKGSRYVVRVVKDGDLLAKQTG
                     LLDGRGRPIRGLPPQVVSGGVCDAEAAWRGAFLAHGSLTEPGRSSALEVTCPGPEAAL
                     ALVGAARRLGVQAKAREVRGVDRVVIRDGDAIGALLTRLGAHDSVLAWEERRVRREVR
                     AAANRLANFDDANLRRSARAAVAAGARVKRALELLGEEAPDHLVAAGHLRIEHAQASL
                     EELGALADPPMTKDAVAGRIRRLLAMADKRASDLGQPGTEAGLTPDMLVP"
     gene            1432..2442
                     /gene="gap"
                     /locus_tag="VGD55_09270"
     CDS             1432..2442
                     /gene="gap"
                     /locus_tag="VGD55_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0870578.1"
                     /translation="MTIRVGVNGFGRIGRNFYRAVAASHGSNIEIVAVNDLTDTKTLA
                     HLLKYDTVLGTFAGQVSHGEGSITVDGKEIKVLSQRDPALLPWGELGVDIVIESTGHF
                     TDADKAKAHITAGAKKVIISAPAKGEDGTFVLGVNDDSYDPAVHHIISNASCTTNCVA
                     PPAKVLQDSFGIVKGFMTTVHAYTNDQVILDFPHKDLRRARAAAQNIIPTTTGAAKAV
                     ALVLPELKGKLDGFALRVPVPDGSITDLVVELEREVTRDEVNAAYKAAAEGPLKGYLV
                     YTEDEIVSSDIVGTPASCTFDSKLTMVSGKTVKVLGWYDNEWGYSSRLADLTALVASR
                     LG"
     gene            2454..>3093
                     /gene="pgk"
                     /locus_tag="VGD55_09275"
     CDS             2454..>3093
                     /gene="pgk"
                     /locus_tag="VGD55_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0870579.1"
                     /translation="MRSVDDLEVAGRTVLVRADLNVPLDSSGGGIPVITDDKRIQASV
                     PTIRDLVGRGAKVVVMAHLGRPKGEVKPELSLRPAAARLSELLGQPVGFPDDANDGIA
                     GTAAQAAVAATAQGGVVLLENLRFEAAETSKDDAERAVFAAQLAALAGEGGSYVGDGF
                     GAVHRKHASVYDLPQLMPHAAGGLVLAEVSVLRRLTDDLARPYVVVLGGAKVS"
BASE COUNT          511 a          997 c         1090 g          495 t
ORIGIN      
        1 tcgggtcgat gtcgtgcttg ctgaccagag ggttgttgcc aaggccgata cgttgcgatc
       61 agcggctgcg gagttagggg gccggttggt gctggcgccc gtcgcgatga gcgacggaag
      121 cccgcgtcac gaccccgaac tgctcgcggc ggcgtatgcg cggatcttcg agatcgactg
      181 atgttcgaaa gcaactaagg ggaagaacca tggcgatgac gtcggcggtc aaggacgagt
      241 tgtcgcggtt gaccgtcacc aagtcgtgct gccgcaaggc ggagacgtcg gcgctgctgc
      301 gcttcgccgg gggcttgcac ctggtcggcg gtcacgtcgt cgtcgaagcc gaactcgaca
      361 ccggatcgtc ggcccgacgg ctgcgcaagg acattgccga gctctacggc cacggatccg
      421 acatcgcggt ggtcgcgccc ggtgggttgc gcaagggcag tcggtacgtc gtgcgggtcg
      481 tgaaggacgg cgatctgctg gcgaagcaga ccgggttgct cgacggtcgc ggccgcccga
      541 tccgcgggct gccgccgcag gtcgtgtcgg gcggcgtgtg cgacgcggaa gccgcctggc
      601 ggggtgcttt tctcgcccac ggctcgctca ccgagcccgg cagatcgtcg gcgctggagg
      661 tgacctgccc cggccccgag gcggcgctgg cgctcgtcgg agcagcccgg cgcctcggcg
      721 tccaggcaaa ggctcgtgag gtgcgcgggg tcgatcgggt ggtgatccgc gatggcgacg
      781 cgatcggcgc gctgctcacc cggctcggcg cgcacgacag tgtgctggcc tgggaagagc
      841 ggcgggtgcg gcgcgaggtc agggcggcgg cgaaccggct cgcgaacttc gacgacgcga
      901 acctgcggcg gtcggccagg gcggcggttg cggcgggtgc gcgggtgaag cgagccctcg
      961 aattgctggg cgaagaggct ccagaccacc tggtcgcggc cggtcatctg cggatcgagc
     1021 acgctcaggc gtcgcttgag gagctcggcg cgctcgccga cccgccgatg actaaggacg
     1081 ccgtggcggg ccgcatcagg cgcctgctcg cgatggccga caagcgcgcg agcgacctcg
     1141 gtcagcccgg caccgaggcg ggccttaccc ctgacatgct ggtcccctga cgtccgccgc
     1201 cagacccgcg cgtcgatcac gtttacgtga tccagacccc cgaagacagg cgtagaagcc
     1261 tcgggagggg gaccgcggcc tgtccaagga gatcatttac aagggacggc ggccggacgt
     1321 cgcggagtgg ccgttatcgg gaccgttacc ggggaatccg gtgcaaagcg cccggtgagg
     1381 atcggttagt ctcaggttgc agaggatctt tctcgaatga ggagctcagt cgtgactatt
     1441 cgtgtcggcg tcaacggttt cggccgcatc ggacgcaact tctaccgtgc cgtcgccgcc
     1501 tcccatggca gcaatatcga gatcgtcgcc gtcaacgacc tgacggacac caagaccctg
     1561 gcgcacctgc tgaagtacga caccgtgctc ggcaccttcg ccggtcaggt cagccacggg
     1621 gaaggctcca tcacggtcga cggcaaagag atcaaggtgc tgtcgcagcg cgaccccgcg
     1681 ctgctgccgt ggggcgagct tggcgtcgac atcgtcatcg agtcgaccgg tcacttcacc
     1741 gacgccgaca aggccaaggc gcacatcacc gcaggcgcga agaaggtcat catctcggcg
     1801 ccggcgaagg gcgaggacgg cacgttcgtg ctgggcgtga acgacgacag ctacgacccc
     1861 gccgtccacc acatcatcag caacgcctcg tgcacgacga actgcgtggc gccgcccgcg
     1921 aaggttttgc aagacagctt cggcatcgtc aagggcttca tgacgaccgt tcacgcctac
     1981 accaacgacc aggtgatcct cgacttcccg cacaaggacc ttcgtcgggc ccgggccgct
     2041 gcgcagaaca tcatccccac gacgaccggg gccgcgaagg ccgtcgcgct cgtacttccc
     2101 gagctcaagg gcaagctcga cgggttcgcg ctgcgcgttc cggttcctga cggttcgatc
     2161 accgaccttg tggtcgagct cgagcgcgaa gtgacccgcg acgaggtcaa cgcggcgtac
     2221 aaggctgcgg ccgaagggcc cctcaaggga tacctcgtct acaccgaaga cgaaattgtg
     2281 tcctccgaca tcgtgggcac accggcgtcg tgcaccttcg actccaagct caccatggtg
     2341 agcggcaaga ccgtcaaggt gctggggtgg tacgacaacg agtgggggta ctccagtcga
     2401 ctcgctgatc tcactgcgct tgtcgcgtca cgactcggct aggggctgcg tcgatgcgta
     2461 gtgtcgacga cctcgaggtc gccggccgga cagtgcttgt ccgggccgac ctcaacgtcc
     2521 cgctcgattc cagcggtggc ggcatccccg tgatcaccga cgacaagcgc atccaggcga
     2581 gcgttcccac gatccgtgat cttgtgggcc gcggggccaa ggtcgtcgtg atggcgcacc
     2641 ttggccggcc gaagggcgag gtcaagcccg agttgtcgtt gcggcccgct gcggcgcggc
     2701 tgtcggaact cctcggccag ccggtcggct ttcccgacga tgccaatgat ggcatcgcag
     2761 gtacggcagc gcaggcagcg gtcgcggcga ctgcgcaggg cggagtcgta ctgctcgaga
     2821 acctgcgctt tgaagcagca gaaaccagca aggacgacgc tgagcgggca gttttcgcag
     2881 ctcaactcgc cgctctggct ggtgagggcg ggtcctacgt cggtgacgga ttcggtgccg
     2941 tgcaccgcaa gcacgccagc gtctacgact tgccgcagct gatgccgcac gcagcgggcg
     3001 gtcttgtgct ggccgaggta tcggtgctgc gcagactgac cgacgacctc gcgcggccct
     3061 acgtggtcgt tctcggcggc gcgaaggtct ccg
//
LOCUS       DASWVF010000525         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_269196, whole genome shotgun sequence.
ACCESSION   DASWVF010000525 DASWVF010000000
VERSION     DASWVF010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269196"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VGD55_09280"
     CDS             <1..637
                     /locus_tag="VGD55_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870580.1"
                     /translation="APDDGPARRVRVLVGLGVAAFFFYTGLEVTAGQWAASFFRGPLG
                     LSAGAAGPAVFAYWGSLTAARFAIAIPKRTPRPELLVRVGCAGGLVASIVIWWAPSTA
                     AVVAGFAIMGAALAPVFPALVTLTPIRIGHARAQHAIGWQIAGANVGAAGISALVGVI
                     LQHEGLRTLGPCLLAIAVIVVACNVALEVLSPRRAAPLESTDDPRGAKVVP"
     gene            634..2199
                     /gene="guaA"
                     /locus_tag="VGD55_09285"
     CDS             634..2199
                     /gene="guaA"
                     /locus_tag="VGD55_09285"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0870581.1"
                     /translation="MSTDGETPTVLVVDFGAQYAQLIARRVREADVYSEIVPHTMSVD
                     EIVARKPAAVILSGGPSSVYADGAPSIDASLFETGIPVFGICYGFQAMAQALGGVVER
                     TGLAEFGGTKVTVAPASDGVLLRDLPTQQSVWMSHGDSVTVAPAGFTVTASSDGAAVA
                     AFEDVSRRLAGVQFHPEVRHTEHGQTMLRRFLREVAGISPNWTTAGIVADQVTRIREQ
                     SGSGRVICGLSGGVDSAVAAALVQRAVGSQLTCVFVDHGLLREGEAEQVERDFVASTG
                     VRLHVVDASATFLAALEGVSDPEAKRKIIGREFIRAFEAAARSISATAPVDFLVQGTL
                     YPDVVESGGGPGTANIKSHHNVGGLPDDLQFALVEPLRTLFKDEVRAVGAELGLPDAL
                     VWRQPFPGPGLAVRIIGSVTAERLAVLREADAIAREELTAAGLDRDIWQCPVVLLADV
                     RSVGVQGDGRTYGHPIVLRPVSSEDAMTADWSRVPYEVLARISTRITNEVREVNRVVV
                     DITSKPPGTIEWE"
     gene            complement(2360..2965)
                     /locus_tag="VGD55_09290"
     CDS             complement(2360..2965)
                     /locus_tag="VGD55_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0870582.1"
                     /translation="MSRQFAVTWDYRCPFARNAHEHLLTGLAAGADWQVTFLPFSLGQ
                     VHVAEGAPSVWEKPEQDSGIVALQAGVVVRDDYPDLFPGVHRALFAARHDEALRLDDR
                     AVVSDALERHGVPVEKVFARIDDGSALATVKAEHEGYAASHDVWGVPTFIVDDQAVFI
                     RFMHRATDGAEPAASIDSVERVVELIGWTDLNEFKHTSVPR"
BASE COUNT          487 a         1011 c         1037 g          582 t
ORIGIN      
        1 tgcgcccgac gacggcccgg cccgccgagt gcgagtgctc gtaggccttg gcgttgctgc
       61 ctttttcttc tacaccggcc tcgaagtgac ggctggccag tgggctgcga gcttctttcg
      121 cggtccgctc ggcctcagtg ccggtgccgc cggacccgcg gtcttcgcct actggggatc
      181 gctgacggcg gcccggttcg cgatcgcaat tcctaagcgg acgccgaggc ctgagcttct
      241 ggtcagggtc gggtgcgccg gggggctggt ggcctcgatc gtgatctggt gggcaccatc
      301 gacggccgcg gtcgtcgcgg gattcgccat catgggggca gcacttgcac cggtctttcc
      361 cgcacttgtg accctcaccc cgatccggat cggtcatgcg cgggcgcagc acgcgatcgg
      421 gtggcagatc gccggggcca atgtcggtgc ggctgggatc tcggctctcg tcggcgtgat
      481 ccttcaacac gagggcttgc gaacgcttgg accctgcctg ctggcgatcg ctgtcatcgt
      541 ggtggcgtgc aacgtggccc tggaggtgct gagcccacgt cgagccgcgc ctctagaatc
      601 aacagacgat ccccgaggtg cgaaggtggt tccgtgagca ccgatggcga gacgcccacc
      661 gttctcgtcg tcgacttcgg ggcgcaatac gcgcagctga tcgcacgccg agtgcgcgag
      721 gccgacgtct acagcgagat cgtcccgcac acgatgtcag tcgacgagat cgttgcccgc
      781 aagccggcag cggtgattct ctccggtggc ccgtcgtccg tttatgccga cggtgcgccc
      841 tcgatcgacg catcgctgtt cgagacgggc atcccggttt tcggtatctg ctatgggttc
      901 caggccatgg cacaggcact cgggggagtg gtcgagcgca ccgggcttgc cgaattcggt
      961 ggcaccaagg tgaccgtggc tccggcgtcc gacggtgtac tgcttcgtga ccttcccacc
     1021 cagcagtcgg tgtggatgtc gcatggcgac tccgtcaccg ttgcccccgc cggcttcacc
     1081 gtgacggcgt catccgacgg cgcagcggtg gctgcattcg aagacgtgtc acgccgactc
     1141 gccggcgtgc aattccaccc cgaggtccga cacaccgaac acggtcagac gatgctgcgc
     1201 cgtttcttgc gcgaggtcgc gggcatttca ccgaattgga cgacggcggg catcgtcgcg
     1261 gatcaggtga ctcgtattcg tgagcagagc ggttcgggcc gcgtcatctg cggcctctct
     1321 ggcggggtcg attcggccgt tgccgcagcg ctcgtgcaac gcgctgtcgg ctcacagctg
     1381 acgtgcgtgt tcgtcgatca cgggttgctg cgtgagggcg aagcagaaca ggtcgagcga
     1441 gatttcgttg cgtccactgg tgttcgtctt cacgttgtcg acgcctcagc cacattcctc
     1501 gctgctctgg agggtgtgag cgacccggag gcgaagcgga aaatcatcgg ccgcgagttc
     1561 atccgcgcat tcgaagcggc ggcccgttcg atcagtgcca ccgcgccagt cgactttctc
     1621 gtgcagggca ctctttatcc tgacgtcgtc gagtcgggcg gtggtcccgg caccgcgaac
     1681 atcaagtcgc accacaacgt gggcggcctt cccgatgatt tgcaatttgc attggtcgag
     1741 ccgttgcgca cgttgttcaa agacgaagtg cgtgcggtcg gtgccgaact cggattgcca
     1801 gacgctcttg tgtggcggca gccgttcccc ggccccgggc ttgcggtgcg catcatcggt
     1861 tcggtcaccg ccgagcggct ggcggtattg cgcgaagccg acgcgatcgc ccgcgaagag
     1921 ctcaccgcag caggtctcga ccgcgatatc tggcagtgcc cggtcgtgct gcttgccgac
     1981 gtccgctctg ttggtgtgca aggtgacggt cgtacctacg gccacccgat cgtgttgcgt
     2041 ccggtgtcat cggaagatgc gatgacggct gactggtcgc gcgttccgta tgaggtgctc
     2101 gcgcgcatct cgacgcgcat cacgaacgaa gtgcgggaag taaatcgggt cgtagtcgac
     2161 atcaccagca agccgcccgg aaccatcgag tgggagtagg gcggctcgca cctgccgttg
     2221 tagcagtctc acgtgccgtt gtagcaagat caaccgccgg gaacctaggc gttgggtcac
     2281 tacaaccggc ccgacgtgtt ccacctcagc gtcgacacga ggtcgccccc agccgtgacg
     2341 ttcggcgtcg actaacgact cagcgcggta cagacgtgtg cttgaactcg ttgaggtcgg
     2401 tccagccgat gagctcgacc acacgctcga cggagtcgat ggacgctgcc ggctcggcgc
     2461 cgtccgtagc tctatgcatg aagcggatga agaccgcctg gtcatcgacg atgaacgtcg
     2521 gcacccccca cacgtcgtgc gacgcggcgt agccctcgtg ctctgccttg accgtggcaa
     2581 gcgccgagcc gtcgtcgatc cgggcaaaca ccttctcgac cggaacgccg tggcgctcca
     2641 gcgcgtcact caccacggca cggtcgtcga ggcgcaacgc ttcgtcatgc cgggccgcga
     2701 acagcgcgcg atgcacgccg gggaacaggt cggggtagtc gtcgcgcacc acgacgccag
     2761 cctggagcgc aacgatgccg ctgtcttgct ccggtttctc ccacacgctc ggcgctccct
     2821 cggccacgtg tacctgcccg agagagaacg gcaggaaggt gacctgccag tcggcgccgg
     2881 cggccaggcc cgtcaggagg tgttcatggg cgttgcgggc gaaggggcac cggtaatccc
     2941 aggtgaccgc gaattgtcta ctcattttca cgacaacctt accgaggccc gggccattcc
     3001 cgcggccggc cgcttaacgc agcgagcggg tcgcgtggtt cggcagcaag acgatcagta
     3061 gcgtcgcaat cggtccgagt agcagcgaga tgatccacca ccacagcccg ctgcggc
//
LOCUS       DASWVF010000526         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_294481, whole genome shotgun sequence.
ACCESSION   DASWVF010000526 DASWVF010000000
VERSION     DASWVF010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294481"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGD55_09295"
     CDS             complement(<1..520)
                     /locus_tag="VGD55_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870583.1"
                     /translation="MTAVLDAAGLGKRYRQRWALRDCTLQVPAGRVVGLVGPNGAGKT
                     TLLHLAVGLLQPSAGRLDVLGARPADSPAQLGRVGFVAQDTPVYASLSVADHLKLGGW
                     LNPRFDAAAAQRRIDQLGLDRRQRAGKLSGGQRAQLALTLAIAKRPELLVLDEPVASL
                     DPLARREFLQHLM"
     gene            complement(517..909)
                     /locus_tag="VGD55_09300"
     CDS             complement(517..909)
                     /locus_tag="VGD55_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0870584.1"
                     /translation="MIEFHLNTASGVSPYLQLVQQVRRALLLGLLAEGDQLPTVKDVV
                     AHLAINPNTVLKAYRELEHSGLVAARAGVGTFVTSTLRDASLAAHGPLRQELERWFGK
                     ARRAGLDDESIEALFQDTFRTLTEQGVA"
     gene            1037..1624
                     /locus_tag="VGD55_09305"
     CDS             1037..1624
                     /locus_tag="VGD55_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870585.1"
                     /translation="MASADLLLHPVRLRIVQAFLGDRALTTTQLAAELGDVPAGSLYR
                     HLALLTKAGVVQVVAERRVRAVVERTYTLRTFAARVDAAEAAAMTSEQHARAFTVFVG
                     GLLADFDRYLASGQPDLQRDGVSYGMLAMWLTDAEYADFLHDVGVLVAPRLANAPGKG
                     RRRRLVSTVMLPARDKDSTVRGSIVKRTTAKGAKK"
     gene            1621..>1936
                     /locus_tag="VGD55_09310"
     CDS             1621..>1936
                     /locus_tag="VGD55_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870586.1"
                     /translation="MIEARDLTKRYGDKLAVDHLSFTVAPGRVTGFLGPNGAGKSTTM
                     RLVLGLDRPETGSATIAGHPYRDHVEPLKVVGALLEAKAVHPGRTARNHLLFLAQTQG
                     IAN"
BASE COUNT          342 a          610 c          651 g          333 t
ORIGIN      
        1 ccatcaggtg ctgaagaaac tcacgccgcg cgagcggatc gaggctggcc accggttcgt
       61 cgagaacaag cagctcgggt cgtttcgcga tcgcgagcgt gagggcgagc tgggcgcgtt
      121 ggccgcccga cagcttgcca gcgcgctgtc tgcggtcgag gccgagctgg tcgatgcggc
      181 gttgcgctgc ggctgcgtcg aagcgcgggt taagccagcc gcccaacttc aggtgatcgg
      241 ccaccgaaag cgaggcgtag acgggggtgt cctgggcgac gaaaccgacg cggccgagtt
      301 gcgccggact atcggctggc cgagcaccga ggacgtcgag tcgcccggca ctgggttgca
      361 gcaggccgac ggcgagatgc agcagcgtgg tcttgccggc gccgttcggc ccgacgaggc
      421 cgacgacgcg gccggccggc acttgcaagg tgcagtcgcg caatgcccac cgttggcggt
      481 aacgcttgcc gagacccgcg gcgtcgagga cggcggtcac gcgacgccct gctcagtcag
      541 ggttcgaaag gtgtcctgaa acagggcctc gatgctctca tcgtcgagcc cggcccgacg
      601 tgcctttccg aaccagcgct cgagttcctg ccgcaaggga ccgtgcgcgg ccagtgatgc
      661 gtcgcgcaac gtcgaggtga cgaaggtgcc cacgcctgcg cgtgccgcga ctaggccgct
      721 gtgttcgagc tcgcggtagg ccttcagcac ggtgttcggg ttgatagcca ggtgcgcgac
      781 cacgtccttg acagtcggaa gctggtcacc ttcggcgagc aatcccagca acaacgcgcg
      841 tctgacttgt tggaccagct ggagatacgg ggagacaccc gaggcggtgt tcagatggaa
      901 ctcgatcacg attgctcctc attgaactag gtaactagca caatagttac ctgtgcatgg
      961 cgccaatagc tagacgtcaa ctgttaccac atacgtgaac attcacattt ctgagaaccg
     1021 gcgatacggt gcaggcatgg cgagcgcaga tctgctactg cacccggtgc gactgcgcat
     1081 cgtgcaggcg ttcctcggtg accgggcgct cacgacgacc caacttgcag ccgagctcgg
     1141 cgacgtccct gctgggagct tgtaccgcca tctcgccttg ctcaccaaag ccggagtcgt
     1201 gcaggtcgtg gccgagcggc gagtgcgcgc cgtcgtcgaa cgcacctaca cactgcgcac
     1261 gttcgcggcc cgcgtcgatg ccgcggaggc tgctgcgatg acttccgaac agcacgcgcg
     1321 tgctttcacg gtgttcgtcg gtgggttgct cgccgacttc gaccgctacc tcgcgtcggg
     1381 ccagccggac cttcaaaggg acggcgtcag ctacggcatg ttggcgatgt ggctcaccga
     1441 cgccgagtac gccgacttcc tgcacgacgt gggtgttctc gttgcaccgc gcctcgcgaa
     1501 cgcgcccggc aagggacgcc gccgccgtct ggtatcgacc gtcatgctgc ccgcgcgtga
     1561 caaggattcg acagtgaggg gttcgatagt gaagcgcacg accgcgaaag gtgcgaagaa
     1621 atgatcgagg cgcgcgatct caccaagagg tacggcgaca agcttgccgt cgaccatctg
     1681 tcgttcaccg ttgcgcccgg cagagtgacc gggtttctcg gtccgaacgg cgcgggcaag
     1741 tcgacgacca tgcggctggt gttggggctt gatcgtccag aaactggcag cgcgacgatc
     1801 gcgggtcatc cgtaccgcga ccacgtcgag ccgctgaagg ttgtcggcgc gttgctcgaa
     1861 gcgaaggccg ttcatcccgg tcgcaccgcg cgcaatcact tgctgttttt ggcccagaca
     1921 caaggaattg ccaaca
//
LOCUS       DASWVF010000527         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_422038, whole genome shotgun sequence.
ACCESSION   DASWVF010000527 DASWVF010000000
VERSION     DASWVF010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422038"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..>1812
                     /locus_tag="VGD55_09315"
     CDS             94..>1812
                     /locus_tag="VGD55_09315"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0870587.1"
                     /translation="MRRLVTVSMLALVVPAIVVAVPVVSAPQPSPRPVAATMHHIAIS
                     PMLALVSATHSQPLTLVAQQSTSNFRAVGLSWRTPPSGTAITLRAQIEVRSGGQWSGW
                     QELDSSDPGTDASSDGADAPAAGAARDGTDPLWVDHGNGVAIRLLSVSGGEPIDLRVD
                     LIDPGTSPADATAGTAPAASSAQADAGQPTILTRTDWGADESLRMNACPTGPQYTGTP
                     QVAFLHHTDTPNNYAPEDVPAIIRSIYAFHVEGEGWCDVGYNFLVDRFGRIWEGRYGG
                     IDKAVLGAHTGGFNTNSFGVSLIGTFNTAQPPQPMIDATAQLMAWKLALSYANPVGTA
                     TLTAAPFSEVRFAAGTKVLFNVISGHRDADQTSCPGNAAYALLPQLRQETLQDMGAGL
                     VNPTSIVTTPRTVAGNGTVHVVAGMLAPGSWQLLVQDASGSTVRTMTGSGAGIDTTWD
                     MTSDAGTPVPAGAYTLTLSGSQNNQTALPWTTSLVVGGVFGSLDTATTQIGQISLSGW
                     AARAADTTPAVLNITIDGALAGSITPSRPRLDVAAIYPQYTGNLGYSASEPATPGYHN
                     LCVIGVD"
BASE COUNT          319 a          615 c          569 g          309 t
ORIGIN      
        1 agcgaaagat caagattcgt aacgatcttg ggcgaatcat gcttaggtgg catagatccg
       61 tcgtttacca cttcggcccc aggagtgaca tcggtgcgac gcctggtcac ggtttcgatg
      121 ctggccttgg tcgtgccggc aatcgtcgtc gccgtgcctg tcgtctcggc gccgcagccc
      181 tccccgcgcc cggtcgccgc cacgatgcac cacatcgcca tcagtccgat gctcgctctc
      241 gtgagcgcaa cccacagcca gccattgact cttgtagccc agcagtcgac gtccaacttc
      301 agggcagttg ggctcagctg gcggactcct ccgagcggca ccgccatcac gctgcgcgcc
      361 cagatcgagg ttcgctcggg tggtcagtgg agcggctggc aggaactcga ctctagtgat
      421 ccgggcaccg atgcgagctc agacggggct gacgccccgg cggcgggggc ggcgcgcgac
      481 ggaactgacc cgttgtgggt cgaccacggc aatggcgtgg cgatccggct gctatctgtc
      541 agcggcggcg agcccatcga cttgcgcgtc gatctgatcg accccggaac ctcacccgct
      601 gacgccactg ctggtaccgc gcccgcagct tcgagtgcgc aggctgatgc cggacaaccg
      661 acgattctga cccgcaccga ctggggtgct gacgagtcgt tgcgcatgaa cgcgtgcccg
      721 accggacccc aatacacggg cactccgcag gtcgctttcc tgcatcacac cgacactccc
      781 aacaactacg caccggagga cgtgccggcg atcatccgct cgatctacgc gttccatgtc
      841 gagggcgaag ggtggtgtga cgtcggctac aacttcctgg tcgaccggtt cgggcgtatc
      901 tgggaaggcc gctacggcgg tatcgacaag gcagtgcttg gcgcgcacac cggcggcttc
      961 aacaccaaca gcttcggcgt ctcgctgatc ggtacgttca acaccgcgca acctccgcag
     1021 ccgatgatcg atgcgaccgc gcagttgatg gcgtggaagc ttgcgctctc gtacgcaaac
     1081 ccggtcggca ccgcaacgct gacggcggcg ccgttcagtg aggtgcgttt cgctgccggc
     1141 accaaggtgt tgttcaacgt gatctcgggg caccgcgacg ctgaccagac cagttgcccg
     1201 ggtaacgctg cctacgcttt gctgccccag ctgaggcaag agacgcttca agacatgggc
     1261 gccggcctcg tgaatccgac cagcatcgtg acgacgcctc gcacggtggc aggcaacgga
     1321 actgtgcatg tcgtcgcggg catgctcgca cctggatcgt ggcagctgct ggtgcaagac
     1381 gcttcaggtt ccacagtgcg cacgatgacc gggtcagggg ccggcatcga cacgacgtgg
     1441 gacatgacga gcgacgccgg cacgccggtg ccggccggtg cctacaccct tactttgagc
     1501 ggcagccaga acaatcaaac ggcactaccg tggacgacgt cactggtcgt cggcggagtg
     1561 ttcgggtcac tcgacacggc aacgacgcag atcggccaga tctcactcag cggctgggcc
     1621 gcccgcgccg ctgacaccac gcccgccgta ctcaacatca ccatcgacgg cgcgctcgcc
     1681 ggctcgatca cgccctcgcg gccgcggctc gatgtcgcgg cgatctaccc gcagtacacc
     1741 ggcaacctcg gttactcggc cagtgagcct gcgacgccgg gttaccacaa cctgtgcgtc
     1801 ataggtgtcg at
//
LOCUS       DASWVF010000528         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_141821, whole genome shotgun sequence.
ACCESSION   DASWVF010000528 DASWVF010000000
VERSION     DASWVF010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141821"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VGD55_09320"
     CDS             <1..1097
                     /locus_tag="VGD55_09320"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870588.1"
                     /translation="GRLPSASDRWKSFGIFDTWATDHLPLRNDAIKANTTVERDIFGE
                     SPDYGANGTATTTTATGVAGISAPQPSAPAANAVTDFPTVVTGIDGWLYYGTDFRDAC
                     HPVLPAPVIAAQLGRLSQIMQRANKKFVMVIAPDKSDIYPEYLPIADQGVTCTSKAQS
                     ALWAALAAKPPAGYLDLHGAILAAKAQAPGTLLYRKLDTHWNGRGALIYGHLLADSLD
                     PTLWQTTTVTPAGDSNANTDLEALLGLRTPEQAPLFKVTRPGVVQTVNSDFQIANTTT
                     DAPLVTDPTLFISDSFTESARFAINPFFQAAGFINSSAAGSAFPSLVQAIQSSKLVVL
                     EIVERDVVGGQAPILQTSVLDHLAASLGEG"
     gene            complement(1099..1866)
                     /locus_tag="VGD55_09325"
     CDS             complement(1099..1866)
                     /locus_tag="VGD55_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870589.1"
                     /translation="MRAALAMEWEKLRPVHKTSRTIALAIGLAVAVSALVALAVASSA
                     STMSVQDRHTFDSVGISLEGVNAAVLAVAAFGILSITRESATGMMVVTFLAQPARLRV
                     LAAKLLTHAGVAGLAGAGACVAAFAVGQTLLSTGGLATGWSAPQLPSALGGGVVYLML
                     VCVWGIALGALVRTSAAAITSSAFLLVVAPILVQVLPTSMVQQASRWLPSQIGVQAMS
                     AHHDPHAFAPWTGLAVLAGYVAVTLIAGAVRMVRRDP"
     gene            complement(1908..>2553)
                     /locus_tag="VGD55_09330"
     CDS             complement(1908..>2553)
                     /locus_tag="VGD55_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870590.1"
                     /translation="TAVEHLRWLAVANDVPRARADDVLETVGLDGVAGRKVGGFSLGM
                     RQRLGIAAALIGDPPVLILDEPINGLDPEGIRWIREFMQELAADGRTVFVASHLMSEM
                     ALTASALVVIGAGRLIAAESVIDFTERAVSSVRVRSPQLEEIELALSIDGVTSWRQGE
                     VLEIVGVDIEYVGATAARIGATLFELSPQRASLEDAFMQLTCDAAEFATRLRAS"
BASE COUNT          486 a          883 c          791 g          393 t
ORIGIN      
        1 tcggtcgatt gccgagtgcg agcgacaggt ggaagtcgtt cgggatcttc gacacgtggg
       61 cgaccgatca tctgccgctt cgcaacgatg cgatcaaggc gaacaccacc gtcgaacgcg
      121 atatcttcgg cgagagcccc gactacggtg cgaacggcac ggcgaccacg acaacagcga
      181 ccggagtggc cggcatatcg gctccacaac cttcagcacc tgctgcgaac gcagtcaccg
      241 acttcccgac cgtggtcacc ggcatcgacg gttggttgta ctacggcacc gatttccgcg
      301 atgcgtgtca tcccgtgctg ccggctccgg tcatcgccgc gcaactcggg cggctctcgc
      361 agatcatgca gcgtgcgaat aagaagttcg tcatggtgat cgcccctgac aagagcgaca
      421 tctatcccga atacttgccg atcgccgatc aaggcgtgac ctgcacctcg aaggcgcagt
      481 cagcgctgtg ggcggctctc gccgcgaaac cgccagccgg ttacctcgat ctgcacggcg
      541 cgatcctggc ggccaaggcg caggcgccgg gtacgttgct ctatcgcaag ctcgacacgc
      601 actggaacgg caggggcgcg ctgatctacg gccacctgct tgccgacagc ctcgacccca
      661 cgttatggca aaccacgacc gtgacgccgg ccggagacag caacgccaac actgacctcg
      721 aggcactgct tggcttgcga acaccggagc aggccccgct gttcaaggtg acccggcccg
      781 gcgtcgtcca aacagtgaac agcgatttcc agatcgcgaa caccaccacc gatgcgccgc
      841 tcgtgaccga tccgacgctg ttcatcagcg actcgttcac cgaatccgca cggttcgcga
      901 tcaatccgtt cttccaagcc gcggggttca tcaactcctc tgcggccgga agcgcgttcc
      961 cttcgctggt acaggccatc caaagctcga agctcgtggt gctcgagatc gtcgaacgcg
     1021 atgtcgtcgg tggtcaggcc ccgatcctac aaacctcggt actcgaccac ctcgccgcca
     1081 gcctcggcga aggctgattc acggatcgcg gcgaaccatc cgcaccgcac ccgcgatgag
     1141 cgtgaccgcg acgtagcctg cgagcacggc gaggccggtc cagggcgcga aggcgtgtgg
     1201 gtcgtgatgc gccgacattg cctggacgcc gatttgcgac ggtagccacc gcgaagcctg
     1261 ctgcaccatg ctcgtgggaa gcacctgcac caagatcggc gcgaccacga gcaggaacgc
     1321 actcgacgtg atcgcggcgg ccgacgtgcg gaccagcgcg ccgagtgcga ttccccacac
     1381 gcacacgagc atgaggtaca cgactccccc gccgagcgcc gagggcaact gcggcgccga
     1441 ccatccggtg gcgagaccac cggtgctgag cagcgtttgc cccacggcga aggcagcgac
     1501 gcaggcgcca gcacccgcaa gcccggcaac gcccgcatgg gtaaggagtt tcgcggcaag
     1561 cacccgcagc cgggcaggct gcgcgaggaa cgtcaccacc atcatgccgg tggcggattc
     1621 gcgggtgatc gacaagatgc cgaacgcggc caccgcaagc acggccgcat tcacaccctc
     1681 aagggagatg ccgacggagt cgaatgtgtg ccgatcttga accgacatcg tcgatgccga
     1741 cgaggcaacc gcgagcgcga caagtgcgct gacggcgacg gcgaggccaa tggcgagcgc
     1801 gatcgtgcgc gacgtcttgt gcacgggccg gagtttctcc cactccatgg caagcgcggc
     1861 ccgcacgtca ggtctcgctc tcgttggctt cggcccgcat gtcaggctca gctcgcgcgc
     1921 aggcgcgtgg cgaactcggc cgcgtcgcac gtcagttgca tgaaggcgtc ttccaacgac
     1981 gcccgttgtg gtgagagctc gaagagcgtc gcgccgattc tggccgctgt ggcaccgacg
     2041 tattcgatgt cgacgccgac gatctcgagg acctcgccct gacgccacga cgtcacgccg
     2101 tcgatcgaca acgcgagttc aatctcctcc agttgcgggc ttcgcacccg caccgagctc
     2161 acggcacgct cggtgaagtc gatcacgctc tcggcggcaa tcaaccggcc ggcaccgatc
     2221 accacgaggg cactcgcggt caacgccatc tcgctcatca ggtgactcgc aacgaagacc
     2281 gtgcggccat cagcggccag ctcttgcatg aactcccgga tccagcgaat tccctcaggg
     2341 tcaagaccgt tgatcggttc gtcgaggatc agcaccggcg ggtcgccgat gagtgcggcc
     2401 gcgatcccga gccgctgccg catgccgagg gaaaagccgc ctaccttgcg acccgcgacg
     2461 ccgtcgaggc cgacggtctc aagcacgtcg tcggccctgg cgcgcggaac gtcgttcgca
     2521 acggcgagcc agcgcaaatg ttctactgct gtt
//
LOCUS       DASWVF010000529         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_498446, whole genome shotgun sequence.
ACCESSION   DASWVF010000529 DASWVF010000000
VERSION     DASWVF010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498446"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGD55_09335"
     CDS             complement(<1..366)
                     /locus_tag="VGD55_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0870591.1"
                     /translation="MSSASDALRSDLAHLYRRAGFGATPAELDAAVTAGYAATVSSLV
                     QRPSASDQGVLATPPPQLSVAPAPKTIASAAERKAYQAQVSSEGQELSLWWLDRMVMA
                     TNPFPEKLTFFWHGHFATAI"
     gene            complement(451..792)
                     /locus_tag="VGD55_09340"
     CDS             complement(451..792)
                     /locus_tag="VGD55_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY0870592.1"
                     /translation="MVEVGRERFEELVAQALDGIPEQFTKAMRNVAVFVEDVPADPHL
                     LGLYVGVPLTERTTSYAGVLPDRIMIYRSTLCAYCDTEEQVIAEVRKTVVHEIAHHFG
                     IDDDRLHELGY"
     gene            886..958
                     /locus_tag="VGD55_09345"
     tRNA            886..958
                     /locus_tag="VGD55_09345"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Glu,seq:ttc)
     gene            1008..1082
                     /locus_tag="VGD55_09350"
     tRNA            1008..1082
                     /locus_tag="VGD55_09350"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Asp,seq:gtc)
     gene            1136..1209
                     /locus_tag="VGD55_09355"
     tRNA            1136..1209
                     /locus_tag="VGD55_09355"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Phe,seq:gaa)
     gene            1285..1764
                     /locus_tag="VGD55_09360"
     CDS             1285..1764
                     /locus_tag="VGD55_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870593.1"
                     /translation="MGPTKIQGLPAHILLVHLVLAAVPIAALVLIAAAVWPAAGKRLG
                     VLTPIVAFGALITVPVTTHAGKWLQARVASTPLILKHVALGKQVLPWMLGLFLVAVLV
                     WLWERSSKPALHGRAGRIVVGVLAVAISVGAVQQVVRTGEAGSRALWTNNFSSQPRN"
     gene            1832..2296
                     /locus_tag="VGD55_09365"
     CDS             1832..2296
                     /locus_tag="VGD55_09365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0870594.1"
                     /translation="MALIVASTAPNFTLEGWYDGVAEFSLAKRRGVPTVLAFYPGDER
                     LVCTRQLCTYSDNISDLHLFDAEVWGISPQSVESHRDFAGAKKLKRPLLTDVESAVAH
                     AYGVVGPLGIRRSVFVIDGEGRIAWRKVTSLGVSIPSTQEIREALRALRPAA"
     gene            2369..>2902
                     /locus_tag="VGD55_09370"
     CDS             2369..>2902
                     /locus_tag="VGD55_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0870595.1"
                     /translation="MPEADDAARTYVWRGVAGPFEVVVDPGVFVPSSTSKVLAEALRV
                     DPGDIVVDAGCGCGVLSLVAAKLGAAAVIGTDFSVAAVNCSTANAERLGLADIVEFRS
                     GNLLEPVAGTRANVVIADVSGIPDVIAQVTGWFPDGKGGGRTGAELPVAMLENVADYL
                     APGGRVYLPTGTIQDDAA"
BASE COUNT          489 a          989 c          940 g          484 t
ORIGIN      
        1 gatcgcggtc gcaaagtgcc catgccagaa aaacgtcagc ttctccggga acggattggt
       61 cgccatcacc atgcgatcaa gccaccagag gctgagctct tggccctcag acgagacttg
      121 cgcctggtaa gccttgcgct ccgccgcgct cgcaatcgtc ttcggcgcgg gggcgaccga
      181 aagctgcggt ggcggcgtcg caagcactcc ctgatcggac gccgatgggc gctgcaccag
      241 cgaactgacg gtcgccgcgt accccgcggt gactgcagcg tcgagttcgg ccggcgtcgc
      301 gccgaaccca gcccggcgat acaggtgcgc caagtcgctg cgcagtgcat cggaggcgct
      361 gctcatgacc cgagcccgct catcatcccc agagtaaagc gctcaaatgt tcgggccgtg
      421 tcaacatcgc gaaactgtcg ggttcgggcg tcagtacccc agttcgtgca gcctgtcgtc
      481 gtcaatgccg aagtgatgag cgatctcgtg gacgacggtt ttgcgcacct cagcgatcac
      541 ctgctcctcg gtgtcgcaat aggcgcagag cgtggaccgg tagatcatga tccggtccgg
      601 caggaccccc gcgtaggagg tcgtgcgctc ggtcagcggc acgccgacgt acaagccgag
      661 caggtgcggg tcggctggga cgtcctccac gaagaccgcc acgttgcgca tcgccttggt
      721 gaattgctcc ggaatgccgt cgagggcctg ggcgacaagc tcctcaaagc gctcacgccc
      781 gacctccacc accccgccag caaaccacgg ggccgtcccc tatgcttgac cagtccccga
      841 cggatgacgc cggggctgtg gcctatcgac gcaaatctgg ctaccgtccc catcgtctag
      901 tggcccagga caccgccctt tcaaggcggc ggcacgggtt cgaatcccgt tgggggcacg
      961 caagtcgcag ttttcagtcc taaaggcacg gccgcagtaa cacggatggt cccgtggtga
     1021 agcctggagt tcacgccgcc ctgtcaaggc ggaggtcgcg ggttcgaatc ccgtcgggac
     1081 cgcaacacgc acgagctgca gttcgaagag cagtggctcc gaattgcagt ggcaaggcca
     1141 ggtagctcag tcggtatgag cgcccgcctg aaaagcggga ggtcgacagt tcgatcctgt
     1201 ccctggccac tccctcgcag cgcctgaacc agtcgcccgc cggagccgtt gcgagaatca
     1261 gagctcacca acggctggag tgtcatgggt ccgaccaaga ttcaaggact gcccgcccac
     1321 atcctgctcg tccatctcgt tctagcggcc gtgccgatcg cggcgctcgt gctgatcgcc
     1381 gctgcggtgt ggccggcggc gggtaagcgg ctcggtgttc tgacgccgat cgtggcgttc
     1441 ggagcgctaa tcaccgtgcc ggtgacgacg cacgccggca aatggctgca ggctcgcgtc
     1501 gcgtcgactc cactgatcct caagcacgtc gcgctcggca agcaggtatt gccctggatg
     1561 ctcgggctgt tcctggtcgc cgtcctcgtg tggctgtggg agcgcagcag caagccggcc
     1621 ctgcacggtc gggccggtcg catcgtcgtc ggcgtcctcg ccgtcgcaat ctcggtcggc
     1681 gcggtgcaac aggttgtgcg taccggcgag gccggctcac gcgcgctgtg gaccaacaac
     1741 ttcagcagcc aaccccgcaa ctaaccgcgc accctcgaac tgacgccacc ctgcgactga
     1801 aggcaaccag gcgggacgcc gaaaagacgg catggccctc atcgtcgcat cgactgcgcc
     1861 gaacttcacc ctcgagggct ggtacgacgg agtcgccgag ttcagcctcg cgaagcgacg
     1921 cggcgtcccg accgtactgg ccttctatcc gggggacgag cggctcgtct gcacccggca
     1981 gctgtgcacc tactccgaca acatctccga cctgcacctc ttcgacgccg aggtgtgggg
     2041 catctcaccc caaagcgtcg agtcacatcg cgactttgcc ggcgccaaga aactcaaaag
     2101 gccgctcctc accgacgtcg agagcgcggt ggcccacgcc tacggcgtcg taggcccgct
     2161 cggaatacgc cgatcggtct tcgtgatcga cggcgagggg cgcattgcct ggcgcaaggt
     2221 cacatcactt ggcgtgtcga tcccatcgac tcaggagatc cgcgaggccc tgcgcgccct
     2281 tcgtcccgct gcctgacatc atttgggtat cgccaccgcg ccattaggct ggggccgcgg
     2341 cggaacactg gtgcagaagg gaagagcgat gccggaggcg gacgacgctg cgcgtacgta
     2401 cgtctggcgc ggcgtagccg ggcccttcga ggtagtagtc gaccctggcg tcttcgtgcc
     2461 gagctcgacc agcaaggtgc tcgccgaggc gttgcgggtc gacccaggcg acatcgtcgt
     2521 cgatgctggc tgcgggtgcg gcgtcctctc cctcgttgcg gccaagctcg gagctgccgc
     2581 cgtcatcggc actgatttct cggtcgcagc ggtcaactgc tcgaccgcca acgccgagcg
     2641 cctcgggctc gccgacatcg tcgagtttcg ctcgggcaac ctcctcgagc cggtcgcggg
     2701 tactcgagcc aacgtggtga tcgccgacgt ctcggggatc cccgacgtga tcgcccaggt
     2761 gaccgggtgg ttccccgacg gcaagggcgg tggccgcacc ggcgccgaac tccctgtggc
     2821 gatgctcgaa aacgtcgccg actacctcgc gccgggcggg cgcgtgtatc tgccaaccgg
     2881 gacgatccag gacgacgcgg cg
//
LOCUS       DASWVF010000530         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_116222, whole genome shotgun sequence.
ACCESSION   DASWVF010000530 DASWVF010000000
VERSION     DASWVF010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116222"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VGD55_09375"
     CDS             <1..1306
                     /locus_tag="VGD55_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870596.1"
                     /translation="TGSLEIAAPQIVEVPASRRARIVSGSVLIVVGVLCAVAFGLGSK
                     AGHMSRFRLSPPGAKVTMPNLVVPARIFALVVGIAVAAAGFWRISKDFSKRAMKWVVA
                     LTIVFVVFSFLCWSVIGKSATPLDLFGSGGLFGQTIFLAVPLILGALAGVMCERSGVI
                     NVAIEGQMLVGAFAGAMFGTISKNAAIGTIAGMLAGALIAALLAVFAIKYVVNQVVLG
                     VVLNLLALGLTGFLYDAVMAPNSNSYNSVGTLSVLKIPLLGNIPIIGPLLFDQTIIVY
                     LTYVLIFAVDILLFRTRWGLRTRAVGEHPKAADTVGINVRRTRYINVLIGGMLAGLAG
                     ASLTIGNSSDFVKNMTGGRGFIALAAVIFGRWSPRGAVGAALLFGFATSLQTVLSFIG
                     TPVQIPSDFLGMLPYLATIFAVAGLVGRVRAPAADGEPYEGR"
     gene            1303..1728
                     /locus_tag="VGD55_09380"
     CDS             1303..1728
                     /locus_tag="VGD55_09380"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0870597.1"
                     /translation="MTPGRTEAAIDWATLRDAARGAMGASYSPYSRYAVGAAALVDDG
                     RIVVGCNVENASYGLTLCAECGVVSALQITGGGTLLALACVDAAGEALMPCGRCRQLL
                     WEHGGADMLIDHVSGPLPLADLLPHAFGPDHLDVDPKGR"
     gene            1730..>2375
                     /locus_tag="VGD55_09385"
     CDS             1730..>2375
                     /locus_tag="VGD55_09385"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY0870598.1"
                     /translation="MDVVDVIRDKRDGNVLNDAAIDWLIDAYTKGAIAEEQMSALLMA
                     IFFNGMSPRELNRWTAAMIESGERMSFDDIGKPTTDKHSTGGVGDKITLPLAPIVAAC
                     GAAVPQLSGRGLGHTGGTLDKLESIPGWRASISNAEMRTQLRDVGAVICAAGAGLAPA
                     DRKLYALRDVTATIESIPLIASSIMSKKIAEGTSALVLDVKVGVGAFMKDVDRAR"
BASE COUNT          365 a          761 c          770 g          479 t
ORIGIN      
        1 cacagggtca ttggagatcg ccgcaccgca gatcgtcgag gtgccggcgt cgcgacgtgc
       61 tcgcatcgtg tcgggctcgg tgctgatcgt cgttggcgtg ctctgcgcag tggccttcgg
      121 cctgggctcc aaagctggcc acatgtcacg gttccggctc tcgccgccag gcgcaaaagt
      181 caccatgccg aacctcgtcg tgcctgctcg gatctttgct cttgtcgtgg gcattgccgt
      241 cgcggccgcc ggattctggc gcatcagcaa ggacttctcg aagcgcgcga tgaagtgggt
      301 cgttgccctg acgatcgtct tcgtcgtctt ctcctttctg tgttggtcgg taatcggcaa
      361 gagcgcgacc ccgctggacc ttttcggttc tggtggcctg ttcgggcaga ccatcttcct
      421 tgccgtaccg ctcatcttgg gcgcgctcgc aggtgtgatg tgcgagcgat ctggggtcat
      481 caacgtcgcg atcgaaggcc agatgctggt cggcgcgttc gcaggcgcga tgttcggcac
      541 gatctcgaag aacgccgcca tcggaaccat cgccggcatg ctcgcgggcg cgctaatcgc
      601 tgcgctgctc gcggtgttcg ccatcaagta cgtcgtgaac caggtcgtgc tcggcgtcgt
      661 cctgaacttg ctcgctcttg ggttgaccgg cttcctttac gacgccgtca tggcgccgaa
      721 ctccaactcg tacaacagcg tcggcacgct gtcggtgctg aagattccgc tactgggcaa
      781 catcccgatc atcggcccgt tgctgttcga ccagaccatc atcgtgtacc tgacctacgt
      841 gctgatcttt gccgtcgaca tcttgctgtt ccgtacccgt tgggggctgc gcacacgcgc
      901 ggttggcgag caccccaagg ctgccgacac ggtcggcatc aacgtgcgac gcacccgcta
      961 tatcaacgtg ctcatcggcg gcatgctggc cggtcttgcc ggtgcttcgt tgaccatcgg
     1021 caacagttcg gacttcgtga agaacatgac cggcgggcgc gggtttattg cgctcgcggc
     1081 ggtcatcttc ggccgttgga gtccgcgcgg cgcggtgggc gcggccctgc tgttcggctt
     1141 cgcgacgtcg ctgcaaaccg tgctttcgtt cattggtacg ccggtccaaa tcccgtcgga
     1201 cttcctcggc atgctgccat acctcgccac catcttcgcc gtggccgggc tggtcggacg
     1261 tgtgcgcgct cctgccgccg acggtgaacc ctacgagggc cgatgacccc aggtcgtacg
     1321 gaggctgcga tcgactgggc gaccttgcgt gacgcagcac gaggcgcgat gggcgcctcg
     1381 tactcgccgt actcgcgcta cgcagtcggt gccgcggcac tcgtcgacga cgggcggatc
     1441 gtcgttggct gcaacgtcga gaatgcctcg tacggcctca ccttgtgcgc ggaatgcggc
     1501 gtcgtctctg ccttgcagat caccggcggc ggcacgctcc tcgcactggc atgcgtcgac
     1561 gctgccggcg aggcattgat gccgtgcggg cgatgccgcc agttgctgtg ggagcacggg
     1621 ggagcagaca tgttgatcga ccacgtgtca ggtccgctgc cccttgctga cttgctgccg
     1681 cacgcgttcg gccctgacca tctcgacgtc gatccgaaag gtcgctgacg tggacgtcgt
     1741 cgacgtcatc agggacaagc gcgacggcaa cgttctcaac gacgcggcca tcgactggct
     1801 gatcgatgcc tatacgaaag gcgccatcgc cgaggagcag atgtcggcgc tgctcatggc
     1861 gatcttcttc aacggcatga gcccacgaga actcaatcgc tggacggcgg cgatgatcga
     1921 gtcgggtgag cgcatgtcgt ttgatgacat cggaaaaccc actaccgaca agcattcgac
     1981 cggcggagtt ggcgacaaaa tcaccctgcc gctggcaccg atcgtggcgg catgtggcgc
     2041 cgcggtgccg caactgagcg gccgcgggct tggtcacacc ggtggcacgc tcgacaagct
     2101 cgagtcgatc ccaggctggc gagcttcgat ctccaatgcc gagatgcgca cccagttgcg
     2161 cgacgtcggt gcggtcattt gcgcagcggg cgccgggctc gcgccggctg atcgcaagct
     2221 ctacgcgctg cgcgacgtca ccgcgaccat cgagtcgatt ccgctgatcg caagctcgat
     2281 catgagcaag aagattgccg agggcacgtc cgctctggtg ctcgacgtga aggtcggcgt
     2341 cggggctttc atgaaggacg tcgaccgggc gcgcg
//
LOCUS       DASWVF010000531         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_422119, whole genome shotgun sequence.
ACCESSION   DASWVF010000531 DASWVF010000000
VERSION     DASWVF010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422119"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGD55_09390"
     CDS             <1..1051
                     /locus_tag="VGD55_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0870599.1"
                     /translation="LFRSGLQVRAIVALSPEDVLFSASTEPTEQHLWGYFSDGKCVQL
                     TSEAGVHGGTGASDVLVLSSASLSSDGVRTVVRSNGVEVGQIASFGEPAPFSPRVSIS
                     ARGRREVRTALVLPRDHIEGTSALPVLMDPYGGPHAQRVVASRGAYLNAQWLADQGFA
                     VVIADGRGTPARGLAWERAIAGDFATAVLEDQIDALHAVAADHSDLDLTRVGIRGWSF
                     GGYLAALAVLRRPDVFHVAVAGAPVTDFRLYDTFYTERYLGTDTSAASYAVSSLLGDA
                     AGLTRPLMIVHGLADDNVVAAHTLRLSSALLAAGRAHSVLPLSGVTHMTSQEDVAANL
                     VKLEVEFLKNALSHS"
     gene            complement(1060..>2566)
                     /locus_tag="VGD55_09395"
     CDS             complement(1060..>2566)
                     /locus_tag="VGD55_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0870600.1"
                     /translation="KKLPVVLVINKVDRADARIAEVVDETYELFIDLMDDDVDHSSIL
                     DFPIVYACAKAGRASLNRPVDGTMPDSPDLEPLFQTILKTVPPPSFDPDVPLRAHVTN
                     LDASQYLGRLALLRVHSGTMKRGQQVAWCKSDGTIEKVRLTELLGTQALERVPIETAG
                     PGDIVAIAGIPEITIGETIADPDDPRPLPIITVDEPSLSMTVGINTSPLAGETGKKLT
                     ARLVKNRLDAELVGNVSIRVLTTERPDTWEVQGRGELQLAILVEIMRREGFELTVGKP
                     QVVTKRVDGKIHEPMERLTIDAPSDYQGVLIQLLALRKGRLEQMVDHGTGWIRMEYIV
                     PARGLIGFRTEFLTETRGTGLLHHVHEKYEPWHGELRTRPTGSLVADRRGPTTGFALS
                     NLQERGTMFVGPQTEVYEGMIVGENSRADDMDVNPTKEKKLTNMRQSSSDVLVPLIPH
                     KVLSLEQALEFCREDECVEVTPGAVRIRKVELSMSEREKYRGRRAKAARED"
BASE COUNT          410 a          823 c          848 g          485 t
ORIGIN      
        1 gctcttccga tctgggttgc aggtgcgggc gatcgttgcg ttgagtcccg aggacgtttt
       61 gttcagcgcg tcgaccgagc cgaccgaaca gcacctttgg ggatacttca gcgacggcaa
      121 gtgcgtgcag ttgacctccg aagcgggagt gcacggcggg acgggggcca gcgacgtgct
      181 cgtcttgagc agcgcttccc tatcgagcga cggtgtccgg accgtcgttc gctcgaacgg
      241 ggtcgaggta gggcagatcg cgtcgttcgg ggagcccgcg ccgttctcgc ctcgcgtctc
      301 gatctccgcg cggggacgcc gcgaggtgcg caccgccctc gtgttaccgc gtgaccacat
      361 cgaaggaacg tccgccctgc ccgtcttgat ggacccgtac ggcggccccc acgcgcagcg
      421 ggtcgtggcg tcgcgcggcg cgtacctgaa cgcgcagtgg ctggccgacc agggattcgc
      481 ggtcgtgatc gccgacggtc gcggcacacc ggcacggggc ctggcatggg agcgcgcgat
      541 cgccggagac ttcgcaacag cggtgctgga ggatcagatc gatgcgctgc acgctgtcgc
      601 ggccgatcac agcgacctcg atctcacgag ggtgggcatt cgcggttggt cgttcggcgg
      661 ctaccttgcg gcgctggccg tgctgcggcg tcccgacgta tttcatgttg ctgtcgccgg
      721 agcgccggtt actgacttcc ggttgtacga caccttctac acggagcggt acctcggcac
      781 cgacacgtcg gccgcttcct atgcggtgag ctccctgctc ggcgacgccg cagggctgac
      841 ccggccgctg atgatcgtgc acggcttagc cgacgacaac gtcgtagcgg cgcacacctt
      901 acggttgtcg tcggcgttgt tagctgccgg ccgagcgcac agcgtgctac cactctcggg
      961 cgtgacccac atgacgtcgc aggaagatgt ggcagccaac ctcgtcaagc tcgaggtcga
     1021 gttcctgaag aacgcgctat cgcatagcta gtcccagagc tagtcctcgc gagcggcctt
     1081 ggcccggcgt ccgcgatatt tctctcgctc tgacatcgac agttcgacct tgcggatgcg
     1141 caccgctccc ggcgtcacct cgacgcactc gtcctcgcgg cagaactcga gcgcctgctc
     1201 gagtgacaac accttgtgcg ggatcagcgg cacgagcacg tcgctcgacg attgccgcat
     1261 gttcgtgagc ttcttttcct tggtggggtt gacgtccatg tcatcagcac gcgagttctc
     1321 gccgacgatc atcccctcgt agacctcggt ctgcgggccg acgaacatcg tgccgcgttc
     1381 ctggaggttc gacagtgcga agcccgttgt tgggccgcgc cggtcggcaa cgagcgaacc
     1441 ggtcgggcgg gtacgcaact cgccgtgcca cggctcgtac ttctcgtgca cgtggtgcaa
     1501 caggccggtg cctcgtgtct cggtgaggaa ctcggtgcgg aacccgatca gcccacgcgc
     1561 cggcacgatg tactccatcc ggatccagcc ggtcccgtgg tcgaccatct gctcaaggcg
     1621 gcccttgcgc aatgcaagga gctggatcag cacgccttgg tagtcagaag gcgcgtcgat
     1681 cgtcaaccgc tccatcggct cgtggatctt gccgtcgacc cgcttcgtga cgacctgcgg
     1741 cttgccgacc gtgagttcga agccttcacg ccgcatgatc tcgacgagga tcgcgagttg
     1801 gagctcgccg cgaccttgga cttcccacgt gtcaggtcgc tcggtggtga gcacacggat
     1861 agagacgttg ccgacgagct cggcgtcgag acggttcttc accaaccgtg ctgtgagctt
     1921 cttgcccgtc tcaccagcaa gcggtgaggt gttgatgccg accgtcatcg acaagctcgg
     1981 ctcgtcgacc gtgatgatcg gcagcggacg cgggtcgtcg ggatcggcaa tggtctcgcc
     2041 gatcgtgatt tcggggatgc cagcgatggc aacgatgtcg ccagggcccg cggtctcgat
     2101 cggcacccgc tcgagcgcct gcgtaccgag caactccgtg agccggacct tctcgatcgt
     2161 gccgtcggac ttgcaccagg ccacctgctg cccgcgcttc atcgtgcccg aatgaacgcg
     2221 cagcagcgca aggcgaccga ggtactgcga cgcgtcgagg ttcgtcacat gcgcacgcaa
     2281 tggcacatcg ggatcgaagg acggcggcgg aacggtcttg agaatggtct ggaacaatgg
     2341 ctcgaggtcg gggctatcgg gcatcgtccc gtcaactggt cggttcaacg atgcgcggcc
     2401 cgccttcgca catgcgtaga cgatcgggaa atcgaggatg ctcgagtggt cgacgtcgtc
     2461 gtccatcaga tcgatgaaca actcataggt ctcgtcgacg acctcggcga tgcgcgcgtc
     2521 ggcgcggtcg accttgttga tgacaaggac gacggggagc ttcttc
//
LOCUS       DASWVF010000532         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_345540, whole genome shotgun sequence.
ACCESSION   DASWVF010000532 DASWVF010000000
VERSION     DASWVF010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345540"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VGD55_09400"
     CDS             <1..730
                     /locus_tag="VGD55_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0870601.1"
                     /translation="IEAVGADVAGYEVGDRVFGVVTKPYLGDGGFADYVTVATAVGLA
                     KLPASIEFADAGVLGLAGVAAISAFDKANIQPGQVVLIAGATGGVGSQALQLAVKAGA
                     TVIATAVTAEEKQLVTDLGAAETVDYNEPIAPAVHANYAAGVDVVLHFAGDAGAVLAA
                     ARKGGTFVSTLIMSAEQLPSPDVTVLGVYANPDEATLNRVATNHAKGVTRVRIERTYS
                     LDEVPDALAAFATGTLGKLAITVA"
     gene            complement(742..1569)
                     /locus_tag="VGD55_09405"
     CDS             complement(742..1569)
                     /locus_tag="VGD55_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870602.1"
                     /translation="MSTLTAARTTDLGPGRVTQASVIRSEWIKVRSLRSTWYSLLAAL
                     LVIDGLGTLFSALHAHRVESGNPAGSGIQIDVTQVSLRGVFLAQLAIGVLGVLVVTGE
                     YSTGMIRSSMAAVPRRQPVLIAKAAVFAALVFVVSLVGTLAAFLLGQRAQASTHEQAT
                     LSTPGAVRAIVGGALFLTLIGLLAVGLGFLIRSTAGAIATLFGIVLVAPILTNALPAP
                     YSNDVAKYLPLNAGTQILTTMHQDPGMLGPWAGIGVTALYAVVALLAGLFMLKRRDV"
     gene            complement(1566..>2417)
                     /locus_tag="VGD55_09410"
     CDS             complement(1566..>2417)
                     /locus_tag="VGD55_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870603.1"
                     /translation="GPNGAGKSTTMRLLLGLDRPSAGGSLINGKPYADHPAPLREVGA
                     LLEARAVHTGRSARNHLLAMAATNRIPRARVDEMISMVGLEDVAGKRVGGFSLGMGQR
                     LGIASALLGDPGVLLLDEPVNGLDPEGILWIRNLLKALAAEGRTVFVSSHLMSEMAVT
                     ATELIVVGRGKLITSGTVATVIGAATGGAVLVRTTEADRLAALLVGDGYHAQRVAHDT
                     LHVTGTDSTEIGLVAARNAIALMELTPQQATLEEAFMEITRDAVEFHGTSVANVPAYS
                     AAADGSR"
BASE COUNT          439 a          779 c          794 g          405 t
ORIGIN      
        1 gatcgaggcg gtcggcgccg acgtggcggg ctatgaggtc ggcgatcgag tcttcggcgt
       61 ggtgaccaag ccctacctcg gcgatggtgg cttcgccgac tacgtcacgg tcgcgacagc
      121 agtcggcctc gcgaagttgc cggcgagcat cgaattcgcg gacgccggcg tgctggggtt
      181 ggccggcgtc gcagccatca gtgctttcga caaggcgaat attcaacctg gtcaggtcgt
      241 gctcattgcc ggcgcgaccg gtggcgtcgg gagccaggcc ctccagctcg ccgtgaaagc
      301 gggcgcgacc gtcatcgcta ctgctgtgac cgctgaagag aagcagttag ttactgacct
      361 cggcgcagct gagaccgtcg actacaacga accgatcgca cccgccgtcc acgcgaatta
      421 cgctgctggt gttgatgttg tgctgcattt tgctggtgat gccggtgccg tgctggcggc
      481 cgcgcgcaag ggtggcacct tcgtgtcgac tctgatcatg tcggcggaac aactgccgag
      541 tccggatgtc actgtgctgg gcgtgtacgc aaatcccgac gaggccacgt tgaaccgcgt
      601 cgcaaccaat cacgccaagg gtgtgacgcg tgtgcgcatc gagcggacgt actcactcga
      661 cgaagtaccc gatgcgctcg cagctttcgc caccggcacg ctgggcaagc tcgccatcac
      721 cgtcgcctaa gcggccgtcg atcagacgtc gcgccgcttg agcatgaaga ggccggcgag
      781 cagggcaacg accgcgtaca gggcagtgac gccgattccg gcccatggcc ccagcatccc
      841 cgggtcttga tgcatggtgg tgaggatctg cgtcccggcg ttcaggggaa ggtacttcgc
      901 gacgtcgttt gagtacggtg ccggtagcgc gttcgtgagg atcggcgcga cgagaacgat
      961 gccgaacagg gtcgctatcg ctcccgcggt actgcgtata aggaagccga gcccaaccgc
     1021 gagtagcccg atgagcgtga gaaaaagggc cccacccaca atcgcccgca cggcgcccgg
     1081 ggtgctcagg gtcgcctgct cgtgagtgct cgcttgggct cgctgaccga gaaggaaggc
     1141 ggccagcgtg ccaaccaggg agacaacgaa aaccaacgcc gcgaacactg ccgctttggc
     1201 gatcaacacg ggttggcgac gtggtacagc ggccatcgac gaccggatca tgccggtgct
     1261 gtactcgccg gtgaccacga gcacgccgag cacgccgatc gccagttgtg cgaggaacac
     1321 accgcgcagg ctgacttgcg tcacgtcgat ctggatccct gaacccgcag ggttaccgct
     1381 ctccactcgg tgggcatgca gcgcgctgaa aagcgtcccg aggccatcga tgacgaggag
     1441 ggccgcgagc aggctgtacc aggtcgagcg cagcgatcgc accttgatcc actcggaccg
     1501 gatcaccgag gcctgggtca ctcgacctgg accaaggtca gtggttcgcg ccgccgtcag
     1561 ggtactcatc gcgacccgtc cgcggcggct gagtacgcgg ggacattggc gacgctagtg
     1621 ccgtgaaact caaccgcgtc acgcgtgatc tccatgaacg cttcctccag agtcgcttgc
     1681 tgaggagtga gttccatcag cgcgatggcg ttgcgggcag ctaccaggcc gatctcggtg
     1741 ctgtcggttc cggtgacatg gagcgtgtcg tgcgcgactc gctgagcgtg gtacccgtcg
     1801 ccgacgagca gcgccgctaa ccgatccgcc tctgtcgtgc gaaccagcac cgcgcccccg
     1861 gttgctgcac cgatcaccgt ggctacggtc cccgaggtga tgagcttgcc gcggccgaca
     1921 acgatcagct cagtcgcagt gaccgccatc tcgctcatga ggtgcgagga gacgaacacc
     1981 gtgcgaccct cggcggcgag agccttcaac aggttgcgga tccacaagat cccttcggga
     2041 tccaagccgt tcactggctc gtccagaagg agcacgcccg ggtcgcctaa tagcgccgat
     2101 gcgataccga ggcgctggcc catgccaagg gaaaatccgc cgactcgctt acctgcgacg
     2161 tcctcaagac caaccatgct gatcatctcg tcgactcgcg cccgcgggat gcggttggtc
     2221 gcggccattg ccagcaggtg attgcgggca gaacggccgg tgtgaacggc gcgagcctca
     2281 agtagcgcgc ccacctcgcg caacggcgcc ggatgatccg cgtagggctt cccgttgatg
     2341 agcgagccac cagcgcttgg tcgatccagt ccgagcaaca gacgcatcgt cgtcgacttc
     2401 cccgcgccgt tcggacc
//
LOCUS       DASWVF010000533         7630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_294650, whole genome shotgun sequence.
ACCESSION   DASWVF010000533 DASWVF010000000
VERSION     DASWVF010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294650"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGD55_09415"
     CDS             complement(<1..954)
                     /locus_tag="VGD55_09415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870604.1"
                     /translation="MAPAIEIVVPVHNEEHDLEPSVRRLHDYLTANFPLSFRITIADN
                     ASTDATSMIAAALASELPEVVSVRLEQKGRGRALRRVWSSSDAVLLAYCDIDLSTDMA
                     ALPALLAPLASAHSELSIGTRLAHGSSVVRGAKREFISRCYNLVLRTALGVRFSDAQC
                     GFKAIRADVAREILPLIEDNCWFFDTELLVLAERAGLRIHEVPVDWVEDPHSSVDIKA
                     TALADLRGVWRVGWALSTGAVQLDPLRQRFGRSRGAPAPAALGARVRRFALIGLLSTC
                     VQLLLFLLLRGPFGAIPANAVALLLATVANTAANRRFTFGLR"
     gene            complement(1134..1580)
                     /locus_tag="VGD55_09420"
     CDS             complement(1134..1580)
                     /locus_tag="VGD55_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870605.1"
                     /translation="MVLRTVSGRRTGESSDDLESWLVQLQDCPPVTRTVVTICANDVH
                     GSEPATWFYVEADAKTAVARYRCMSCGDSKDVLDSGEHWNFPRMWACPGCSASIAEIA
                     AGLHTDADGVVNWLALAARCVDCGTIEGLTDFNTDALPSDEVLRQL"
     gene            complement(1718..3379)
                     /locus_tag="VGD55_09425"
     CDS             complement(1718..3379)
                     /locus_tag="VGD55_09425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0870606.1"
                     /translation="MRGFVLTTFAELGIAPETAEALSAVGITIPFPVQALAIPLALTG
                     TDLIGQARTGTGKTLAFGAPLLQLIDFVDDPRPRALIVVPTRELAIQVAADLVIAGKG
                     LGARVLAVYGGRSYEPQVAALKAGIDVVSGTPGRLLDLARQGHLDLSDIQVLVLDEAD
                     EMLDLGFLPDVERILALTPASRQTMLFSATMPGEVISLSRKYLKQPTRIRAEEPDEGR
                     TVPETAQFVYRAHSMDKPEVLARILQAQGRGLAMVFCHTKRHCDRLAADLTERGFAAA
                     AVHGDLGQGQREQALRAFRAGKVDVLVATDVAARGIDVEGVTHVINYSCPDDEKTYLH
                     RIGRTGRAGASGVAVTLVDWDDLARWKSINALLDLSFPDPPETYSTSAELHEQLGIAR
                     GVTGVLPRADRTRAGLGAEVLEDVGETGRPRNSAGGSASGPRRGAGGRSAAASSSRSG
                     SSAGARTTKPRTAPGGSTEAPRRQRTRSAAADGAPTERAATAPRDVPSSGASAGSDSS
                     AADGAPRRRRSRGGRGRSGGAKPEQGASPSVGTSTAPPAPRSANS"
     gene            3566..4264
                     /locus_tag="VGD55_09430"
     CDS             3566..4264
                     /locus_tag="VGD55_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEY0870607.1"
                     /translation="MSEERVPDVDLMADPTYRDAVTDLLGVLAYAELVAFERLAYDAR
                     MAPTLEDKAALARLATAEFGHYQLLEHHLEGMGVEAGKAMAPFVVPLEAFHAMTAPSD
                     WAESLVKAYVGDGIAADFYREIAQLLDPKARALVLEVLADTGHAEFAVERVRQAIEAD
                     PTIAGRLALWGRRIVGEALAQAQAVCAEREALVTLLVGGVPGTSADLAELMRTFTRIT
                     DAHMGRMAALGLSA"
     gene            complement(4266..4490)
                     /locus_tag="VGD55_09435"
     CDS             complement(4266..4490)
                     /locus_tag="VGD55_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEY0870608.1"
                     /translation="MEVKIGVQYAAREIVVESGQTPAAVEALVAAAVKDGGLLILADE
                     RGRRVLVPVEKLAYVEIGEASERRVGFGTM"
     gene            complement(4535..5458)
                     /gene="ligD"
                     /locus_tag="VGD55_09440"
     CDS             complement(4535..5458)
                     /gene="ligD"
                     /locus_tag="VGD55_09440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0870609.1"
                     /translation="MQVGDRRLAISNLEKILYPQTGFTKGEVLDYYARIAPALLPHLA
                     GRPLTRKRYPNGVDGPSFFEKNAPRGTPDWVRTVNLPVPGSTMNRETIDFVIVDDLPA
                     LVWTANLAALELHVPQWKVGPRGGVQDPDLLVLDLDPGPPATIVECCEVALLLKEILD
                     ADGLTAYAKTSGSKGLQLYAPIHPTPDDDTRAYARALAKRLEKQRPDLVVSDMTKALR
                     HNKVLVDWSQNAAAKTTVAPYSLRARPEPTVSTPISWAEVSACRTPEQLVFLPDAVLK
                     RIEMDGDLLADLEALPTATRPRLPRPAAPTG"
     gene            5568..6602
                     /locus_tag="VGD55_09445"
     CDS             5568..6602
                     /locus_tag="VGD55_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0870610.1"
                     /translation="MRSIWKGSISFGLVSIAVKVYSATEEKDIAFHQVHRDDGGRIKY
                     KRICSIDGEEVAYNDIAKGYELPDGDVVVLTDDDLAELPLTSNREIDVLQFVPLDQVD
                     PIYFNKSYYLEPDARATKAYVLLRDTLEKSGRVAVVKVALRRREALATLRVREGVITL
                     ETMLWPDEIRAPEFGFLDESVDVRPAETAMASSLVDSLSGDFDPSDFTDQYRAALESV
                     VEAKIEGRDLIPAPDSGEAEASGTVVDLVAALQASIAAVKAGAAAGAAGAAKRSPAAK
                     KPAAAKSLASKSPASKAASPKAATPKAVTPSSTATRAVAKKAATKTPAVAKAVEKAVE
                     KKPVRARRSA"
     gene            6712..7413
                     /locus_tag="VGD55_09450"
     CDS             6712..7413
                     /locus_tag="VGD55_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870611.1"
                     /translation="MSVSDNLDIVAALREVPGVADADVEPDGDGDGLGLLRLGLAPGV
                     DEVEVASAVGRLLRERFGLGVDADRVQLVEDMQADADDPDIEDQEGQDADRFRPAIRR
                     MHIISSGLQVAAEVTLSVGKSARAIGRPVPETSAIGRAEGTATQSGVQRAVATATLRA
                     VEELLGGKARFELDLVELTPTGRDRAVLVALTMLSTNGSEKLTGVAGVREDVRQAAIR
                     ATLDALNRRVENMVS"
BASE COUNT         1267 a         2487 c         2646 g         1230 t
ORIGIN      
        1 gcgaagcccg aaggtgaagc gacggttggc cgcggtgttg gcgaccgtgg ccagcagcaa
       61 cgccaccgcg ttcgccggga tcgccccgaa cggcccgcgc agcagcagga acagcagcag
      121 ctggacacag gtgctgagca gcccgatgag cgcgaaacgc cgcacccgcg cgcccagcgc
      181 ggcgggcgcg ggggcgccgc gagagcggcc gaatcgctgc cgcagcggat cgagctgtac
      241 ggctccggtc gacagcgccc atcccacccg ccacacaccg cgcaggtcgg ccagcgcggt
      301 ggccttgatg tcgacgctgc tgtgcgggtc ctccacccag tccaccggga cctcgtggat
      361 tcgcaggccg gcgcgttcgg ccagcaccag cagctcggtg tcgaagaacc agcagttgtc
      421 ctcgatcagc gggagtatct cgcgggccac gtcggcccgg atcgccttga acccgcactg
      481 cgcgtcggag aaccgcaccc ccagtgcagt gcgcaggacc aggttgtagc agcgcgagat
      541 gaactcccgc ttcgcgccgc gcaccaccga ggacccgtgt gccagccggg tcccgatgga
      601 cagctcggag tgcgcggagg ccaatggcgc cagcagcgcg gggagggcgg ccatgtcggt
      661 ggacaggtcg atgtcgcagt aggcgagcag caccgcatcc gagctcgacc agaccctccg
      721 cagcgcccgg ccccgccctt tctgctcgag gcgtaccgag acgacctcgg ggagctccga
      781 cgcgagcgcg gccgcgatca tcgaggtggc gtcggtgctg gcgttgtcgg cgatcgtgat
      841 gcgaaacgag agcgggaagt tcgctgtcaa gtagtcgtgc agccgccgca cgcttggctc
      901 gaggtcgtgc tcctcgttgt gtaccgggac cacgatttcg atcgcgggcg ccacagtcgc
      961 aaggccgtga gtcagagcgt cgtccgccga cgcgtcgtgg cgctgggggg cagggcgggt
     1021 gggaagcgtc gtcgttgtca tgccatgacc ttcgcccgcc ggcatgcggc gaagctgaat
     1081 gtgacctgac ggcgatgttg gagaaggtcg taggcgcctt acgtcaaagg cagctacagc
     1141 tgacgcaaga cctcgtccga cggcaacgcg tcagtgttga agtcggtcag gccttcgatg
     1201 gttccgcagt cgacgcaacg tgcggcgagc gcgagccagt tcacaacgcc gtcggcgtcg
     1261 gtgtgcagtc cggccgcaat ctcggcgatc gacgccgagc agccagggca cgcccacatc
     1321 cgcgggaagt tccagtgttc cccagagtcg aggacgtcct tgctgtcacc acagctcatg
     1381 cagcgatagc gggcgacggc ggtcttcgcg tctgcctcga cgtagaacca ggtggccggc
     1441 tcagaaccgt ggacgtcgtt cgcgcagatc gtcacgacgg tcctggtgac gggcgggcag
     1501 tcctgaagtt ggaccagcca cgactcgagg tcgtctgagc tctcgcccgt acgacgccca
     1561 gaaactgtcc gtaaaaccac gggtcctcct cgcgtgtgcc cgactccatc gtcggtacgc
     1621 ggggggcccg gcttgaatca gtggaggccc tcagagaggt gaaatatcca ggatcggacg
     1681 tgctgcgctc agccgtcggc ggctgttggc gttgcgatca gctgttcgcg ctgcgcgggg
     1741 ccggtggcgc tgtgctggtg cccacactcg gactcgcacc ctgctcaggc ttggcgccgc
     1801 ccgagcggcc tctgccgcct cgcgaccggc gacgccgcgg ggcgccgtcg gcagcagaac
     1861 tgtcagaccc ggcacttgct cctgacgacg gcacgtccct tggggcggtt gccgcccgct
     1921 cggtgggtgc gccatcggct gctgctgacc tcgtgcgctg ccggcgaggg gcttcggtcg
     1981 agcccccggg tgcggtgcgc ggcttggtgg tacgtgcccc tgccgagctt cccgatcgac
     2041 tgcttgacgc agcggccgac ctgcctccgg cacccctgcg aggtccagac gccgagcccc
     2101 ctgccgagtt tcgaggccgg ccggtctcgc cgacatcttc gagcacttcg gcgccaagac
     2161 ccgcccgcgt gcgatcggca cgagggagca cgccggtgac cccgcgcgca atgcccaact
     2221 gctcgtgcag ttcagcggag gtcgagtagg tctcgggcgg gtcggggaac gacagatcga
     2281 gcagcgcgtt gatcgacttc cagcgggcga ggtcgtccca gtcgaccagc gtcaccgcga
     2341 cgcccgatgc gccggcccga ccggttcggc caatgcggtg caggtaggtc ttctcgtcgt
     2401 ccgggcatga gtagttgatg acgtgggtga cgccttcgac gtcgatcccg cgggctgcca
     2461 cgtcggtggc cacgagcaca tcgaccttgc cagctcgaaa cgccctgagc gcctgctcgc
     2521 gttgaccttg gccaaggtcg ccgtgcaccg cagcggcagc gaatccacgt tcggtcaggt
     2581 cggccgcgag ccggtcgcag tggcgcttgg tgtggcagaa gaccatcgcg agcccgcgac
     2641 cttgtgcctg gaggatccgg gccaacacct cgggcttgtc catcgagtga gcgcgataga
     2701 cgaactgtgc ggtctcaggg accgtgcggc cttcgtcggg ctcttctgcg cggatccggg
     2761 tcggctgctt gaggtacttg cgcgacagcg agatcacctc gccgggcatc gtcgccgaga
     2821 acagcatcgt ctgccggctt gcaggtgtga gggcgagaat gcgctcgacg tcgggcagga
     2881 agcctaggtc gagcatctcg tcggcctcgt cgagcacgag aacctggata tcggacaggt
     2941 cgaggtggcc ctgccgggcc aggtcgagca atcggccagg agtgcccgac accacgtcga
     3001 tgccagcctt cagcgcggct acctgcggct catacgagcg acccccatag acggccagca
     3061 cccgcgcgcc taggcccttg cctgcgatca cgaggtcagc ggcgacctgg atcgcaagtt
     3121 ctctggtcgg gacgacgatc agtgcgcgtg gtcgcggatc gtcgacgaag tcgatgagct
     3181 ggagcaacgg cgccccgaag gccaacgtct tgccggtacc cgtgcgcgcc tgaccgatca
     3241 ggtcggtgcc ggtgagggcc agggggatcg cgagcgcttg gacggggaac ggaatggtga
     3301 tgccgacggc cgacaaggcc tcggcggtct cgggagcaat cccgagttcg gcaaaggtgg
     3361 tcagaacgaa acctctcaaa aggagctgac cctgcggtcc ggacgatcgt ggacgctggg
     3421 gtgttcacag cgctgctcgc agggtccggg cgggagcggg gtgcggggtg cgcccaagct
     3481 gttggtgcag cctaccgcga tctaggaagc ctgcgatcca ggaagcacta cggggcgacg
     3541 gcgcgtcggc actacgcttc ggggcgtgag cgaggagcgc gttcccgacg tcgacctgat
     3601 ggccgacccg acctatcgag acgccgtgac cgacttgctc ggagtgctcg cctatgccga
     3661 gttggtggca ttcgagcggc tggcctatga cgcccgcatg gcaccaacgc tcgaagacaa
     3721 ggcagccctc gcccgccttg cgaccgcgga gttcggtcac taccaactgc tcgagcatca
     3781 ccttgagggc atgggcgtcg aagccgggaa ggcgatggca cccttcgtcg tccccctcga
     3841 ggcatttcat gccatgacgg cgccgagcga ctgggcagag agcctcgtca aggcctacgt
     3901 cggcgacggc atcgcggcgg atttctatcg cgagatcgcg caactgcttg acccgaaggc
     3961 gcgggcgctc gtgcttgagg tcctggccga caccggacac gcggagttcg cggtcgagcg
     4021 ggtgcggcag gcgatcgaag ccgacccgac cattgccgga cgtctcgcct tgtggggacg
     4081 ccgcattgtc ggggaagcac ttgcccaagc tcaggctgtc tgcgcagaac gcgaggccct
     4141 cgtgacgctg ctggtggggg gagtcccagg caccagcgca gatctcgcag aactcatgcg
     4201 gacgttcacc cggatcaccg acgcgcacat ggggcggatg gcggcactcg gcctgtcggc
     4261 ctgagctaca tcgtgccgaa gccgacccgc cgctcggatg cttcgccgat ctcgacgtac
     4321 gccagcttct cgacaggaac caggactcgc cggccacgtt cgtcggccag gatcagcagc
     4381 ccgccgtcct tgaccgcagc ggcgaccagc gcctcgacgg cggctggggt ctggccgctc
     4441 tcgacgacga tctcgcgtgc cgcgtactgg acaccgatct tgacctccac ggtcatcctc
     4501 cgcatgttgc gacgttcgtg ctggtaggtc gaacctaccc cgtcggcgcg gcgggccgcg
     4561 ggagtctggg tcgcgtagcg gtgggcaggg cttcgagatc ggccagcaaa tcgccgtcca
     4621 tctcgattct tttgagcact gcgtctggca ggaaaacaag ctgctcgggt gtccggcacg
     4681 ccgaaacctc ggcccaactg atcggcgtcg agaccgtcgg ttcggggcgc gcccgcagcg
     4741 aatagggcgc cactgtcgtc ttggctgcgg cgttctgact ccagtcgacg agcaccttgt
     4801 tgtggcgcag cgccttcgtc atgtcggaga cgacgaggtc ggggcgctgc ttctcgagtc
     4861 gcttcgcgag ggcgcgtgcg taggcccggg tgtcgtcgtc gggcgtcggg tgaatggggg
     4921 cgtacagctg aaggcccttc gagccgctcg tcttggcgta ggcggtcagg ccgtcggcgt
     4981 cgaggatttc tttcaggagg agcgcgacct cgcagcattc gacgatcgtc gcgggcggtc
     5041 ctgggtcgag atcgagcacg agcaaatccg ggtcttggac gccgccgcgt ggccctacct
     5101 tccactgcgg cacatgcaac tcaagagcgg ccaggttcgc cgtccagacc agtgctggaa
     5161 ggtcgtcgac gatgacgaag tcgatcgttt cgcggttcat cgtgctgccg gggacaggca
     5221 ggttgacggt ccgcacccag tcgggtgttc cgcggggcgc gttcttctcg aagaacgacg
     5281 ggccgtcaac gccgttgggg tagcgtttgc gggtcagcgg gcgccccgcg agatggggta
     5341 gcaacgccgg cgcgatgcga gcgtagtagt cgagaacctc gcccttggtg aatcctgttt
     5401 gtgggtagag gatcttttcg aggttggaga tcgccaggcg gcggtcgccg acctgcacag
     5461 ttgttttgtt tgcttcgccc gagggcatgg tctagggctc cacgaaccga ggcaccaaca
     5521 gaggcacgaa cagaggcacc aacagaggca cgaacagagg atggctcatg cgcagcatct
     5581 ggaaggggtc catctccttc ggcttggtca gcatcgccgt gaaggtgtac tcggcgacgg
     5641 aggagaagga cattgccttc caccaggtgc atcgtgacga cggcggtcgg atcaaataca
     5701 agcgcatctg ctccatcgac ggcgaggaag ttgcctacaa cgacatcgcc aaagggtacg
     5761 agctacccga tggtgacgtg gtcgtactca ccgacgacga ccttgccgaa ctgccgctga
     5821 cgagcaatcg cgagatcgac gtcctccagt tcgtgccgct cgatcaggtc gacccgatct
     5881 atttcaacaa gagctactac ctcgaacctg acgcgcgcgc gaccaaggcc tacgtgctgt
     5941 tgcgcgacac cctcgagaag tccgggcggg tggcggtcgt caaggtggcc cttcggcgcc
     6001 gcgaggcgtt ggcgacgctg cgggtgcgag aaggcgtgat cactctcgag accatgttgt
     6061 ggcccgacga gatccgcgct ccggagttcg gcttcctcga cgagagcgtc gacgtgcgtc
     6121 ccgccgagac cgcgatggcc tcgtcgctgg tcgattccct atcaggcgat ttcgacccca
     6181 gcgacttcac cgaccagtac cgggccgcac tcgaatcggt ggtcgaggcc aagatcgaag
     6241 gccgcgacct gatcccggca ccggattccg gggaggccga agcctctggc accgtcgtcg
     6301 acctggtcgc ggcgctccag gcgagcatcg ctgcggtgaa ggcaggggca gcagccggcg
     6361 ctgccggcgc cgcgaagcga tcaccggctg cgaagaaacc cgcggcggcc aagtctttgg
     6421 cctcgaagtc tcccgcgtcg aaagctgcct ccccgaaagc agcaacgccg aaagctgtca
     6481 cgccgtcgtc caccgctacg cgagccgtcg ccaagaaggc cgccaccaag acgccagccg
     6541 tggcgaaggc ggtggagaag gcggtggaga agaaacccgt gcgagcccgc cgttcggcgt
     6601 aaacgcccgc gcgttcggcg cttaagcagg acggtcccca cgccgacttc agcgtaagag
     6661 gccggcgccc acccgcgtcg tcccaccgct tatcggtcat ttggagccca cgtgagcgtc
     6721 agcgacaacc tcgacatcgt cgcggctttg cgcgaggtgc ctggtgtcgc cgatgccgac
     6781 gtcgagcctg acggtgacgg cgacggatta gggctgctcc ggctcggact cgctcctgga
     6841 gtcgacgagg tcgaggttgc gagcgctgtc ggtcgcctgt tgcgcgaacg gttcggcctt
     6901 ggcgtcgacg ccgatcgtgt tcagttggtc gaagacatgc aggccgacgc cgacgatccg
     6961 gacatcgaag accaagaagg ccaggacgcc gatcggttcc gccccgcgat caggcgcatg
     7021 cacatcatct cttccggact tcaagtcgcc gctgaggtga cgctgtcggt cgggaagtcg
     7081 gcgcgtgcca tcgggcgacc cgtcccagag acgtcagcga tcggccgcgc ggaaggcacc
     7141 gcgacccaaa gcggcgtcca gcgcgcagtg gccaccgcaa ccctgcgagc agtcgaggag
     7201 ttgctcggcg gcaaggcgcg tttcgagctc gacctcgtcg aactgacccc gaccggacgc
     7261 gaccgcgcag ttctggtcgc cctcacgatg ctgagcacga acggcagcga gaagctcacc
     7321 ggagttgccg gcgtgcgcga agacgtccgt caggcggcca ttcgggccac gcttgacgcg
     7381 ctcaaccgtc gcgtcgagaa catggtgagc tagccacctc cttctccaca cgccgccgtc
     7441 cctttcggcg actgaaggaa ttccgggggg tcgcggaccg agaactgccc gaaattcctt
     7501 cactcggcgg atgcgtggta gaccgttgtg ggtggacgcg caaagttggc caggtagcac
     7561 gttcgagacg tcgtgcgggc cggtgttcgt gcgccacgac ccggcggcaa gtgacctgga
     7621 cgccgcgggc
//
LOCUS       DASWVF010000534         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_116368, whole genome shotgun sequence.
ACCESSION   DASWVF010000534 DASWVF010000000
VERSION     DASWVF010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116368"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGD55_09455"
     CDS             complement(<1..480)
                     /locus_tag="VGD55_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870612.1"
                     /translation="MRIAVVGPTHPHTGGIVHHTTELSHQLAAAGHDVDLISWSAQYP
                     GFLHPGTLRVPGGEPEVRVYPRTSYTLAWWNPITWWRTGVRLRLYDAVVFVHASTVQV
                     PAYLTMLRALRAGSGRSLTPRVVVVAHNVFPHERHFGDAVLVRALLSRAAGVLVHSPE
                     "
     gene            complement(485..1477)
                     /locus_tag="VGD55_09460"
     CDS             complement(485..1477)
                     /locus_tag="VGD55_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0870613.1"
                     /translation="MAAQPLSVAPETPVAASRFPGGSTQDGEAAPVVVPPRPYVTVVL
                     PCYNEQDHVLDEIARISVALDASGYSYELVAIDDKSTDETLARLRAAEKNYPKLKVVA
                     FRRNGGSGTVRRIGTHDARGQIVVWTDADMTYPNERIPELVRYLDDNPDVDQVVGART
                     SEQGTHKLLRVPAKWVIRKLAEWLSGTSIPDLNSGLRAFRREVSLPYLRLLPPGFSCV
                     TTITMSFLSNQHTVDYVPISYSQRAGHSKFHPLKDAYRYILQVLRMVMYFNPLKVLMP
                     LAIWLLVIGFGKGIFDVVRYHFHLTTNTLLVFVTGLIIGAVALLADLIVRSRGE"
     gene            complement(1628..>1924)
                     /locus_tag="VGD55_09465"
     CDS             complement(1628..>1924)
                     /locus_tag="VGD55_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870614.1"
                     /translation="PYVLQPLAFNAGYTPAVGKTVDVVRGDAFADALVAGPVAGHHKN
                     VIVMTADPATVGGGASSFLHSAVEVGVVQTVQAIGGTSALSDATLAAVTQSLGG"
BASE COUNT          384 a          626 c          626 g          288 t
ORIGIN      
        1 ctcaggagaa tgcacgagca cacctgccgc gcgcgacagc aacgcacgca ccagcacggc
       61 atcgccgaaa tgccgctcgt gcgggaaaac gttgtgggcg accacgacga cgcgcggcgt
      121 cagcgagcgt ccgctgccag cccgcagcgc ccgcagcatg gtcaggtagg cgggcacctg
      181 aacggtcgag gcgtgcacga acacaacagc gtcatacaga cgaagccgga cgccggttcg
      241 ccaccaggtg atcggattcc accaggccaa ggtgtacgac gtgcgcgggt agacgcgcac
      301 ctcgggctcg ccgccgggca cgcgcaacgt gcccggatgc aggaagcccg ggtactgcgc
      361 cgaccaggag atgaggtcga catcgtgacc ggcggcagcg agttgatggc tcaactcggt
      421 ggtgtggtgc acgatgccgc cggtgtgcgg gtgcgtcggc ccgacgacag cgattcgcac
      481 gtcgtcactc tccccgcgag cggactatca ggtcagcgag caacgccacg gcgccgataa
      541 tgagcccggt gacgaacacc agaagtgtat tcgtcgtcaa atggaagtga taacgcacca
      601 cgtcgaagat gcccttgcca aagccgatga cgagcagcca aatcgccaac ggcatgagca
      661 ctttgagcgg attgaaatac atcaccatcc gcagcacctg aaggatgtag cggtacgcgt
      721 ccttcaacgg gtggaatttc gaatggccgg cccgttggga gtaggagatc ggaacgtagt
      781 cgacggtgtg ctggttcgac aggaacgaca tcgtgatcgt ggtgacgcag gagaatccgg
      841 gtggcagcag ccgaagatac ggcagcgaca cctcgcgccg gaacgcacga agccccgagt
      901 tgaggtcggg aatcgaggta cccgacagcc attcggcaag cttgcggatc acccacttcg
      961 ccggcacccg cagcagcttg tgggtgccct gctcgctggt acgcgcaccg accacctgat
     1021 cgacgtcggg attgtcgtcg aggtagcgca cgagctcggg gatccgctcg ttcgggtagg
     1081 tcatgtcagc gtcagtccat acgacgatct gcccgcgggc gtcgtgcgtc ccgatacgcc
     1141 gaaccgtgcc agacccaccg ttgcggcgga acgccaccac cttcagcttc gggtagttct
     1201 tctcggcggc gcgtagtcga gcgagagtct catccgtcga cttgtcgtcg atcgccacca
     1261 gctcgtagct gtacccgctc gcgtcgagcg caacactgat gcgggcgatc tcgtccagca
     1321 cgtgatcctg ctcgttgtaa cacggcagca cgacggtgac ataaggccga ggcgggacga
     1381 cgacgggtgc cgcttccccg tcttgcgtgc taccgcccgg aaagcgcgac gcagcgaccg
     1441 gggtctccgg cgcgacactc aacggctggg cagccacgac aggtcctccg acgctggagc
     1501 acaggcaaag atcaccggaa gtttaccctc cggagggact cgcacccctg atagctccct
     1561 gtgaaccacc tgtgccccca gaagtcacaa actgcgaccc ggcaccccaa agaaccgtcg
     1621 cgagcggcta gccgccgagc gattgcgtga ccgcagcgag cgtggcgtcg ctgagggcac
     1681 tggtgccgcc gatggcctgg actgtctgga cgacaccgac ctcgacggcg ctgtgcaaga
     1741 agctcgacgc accccctccg acggtcgccg gatcagccgt catcacgatc acgttcttgt
     1801 ggtggcccgc gaccggtccc gcgacgagcg cgtcggcgaa agcgtcgccg cgaacgacgt
     1861 caacggtctt gccgacggct ggcgtgtacc cggcattgaa agccaggggc tgcagcacgt
     1921 acgg
//
LOCUS       DASWVF010000535         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_396778, whole genome shotgun sequence.
ACCESSION   DASWVF010000535 DASWVF010000000
VERSION     DASWVF010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396778"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2010
                     /locus_tag="VGD55_09470"
     CDS             <1..>2010
                     /locus_tag="VGD55_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0870615.1"
                     /translation="AEVAGVLGFAQRSLGLVSPTASTTDVSDAQLPPATGLVKTKLFE
                     SLLQSIETLTQRAPVVLVFEDLQWADSASIEVFDFLTRNLGDRPVLLVATYRSDEVDR
                     GGALRRLLAELGRHSRVSLLELSGLDRVEIASLLTDIVGGTPDWTLVDAVLTRSGGNP
                     FFAEELAAARHAKALPTALRNVVMMRIERLSPHALHVVGVAAAAGTSIHHRLLSATAR
                     IDADELDAAVSEAIDHKVLVADADTGSFRFRHALLREAAYDALLPGERARLHRALAGA
                     LTAHPELGAAGPGYAAMELADHWWEAADWPEALRASVNAADAAAGVFAMPEAYAYFER
                     ALAAGDLTPTDSQVWVGIDRVDLGVKTADAAYMSGETQRSVDLVRRALEALDAGTDPQ
                     RAAICYTMLGRNAWAIGDSALALDSLHAAAALLPADAPSLELAGILAEEARSLMLMSH
                     MDRAEAKAHEAIATARAVGARREEGHALNTLGCIVSDRGDNTRALVLMRESLAIAEEL
                     GNPDQLYRGYNNLAHVLSQAGQLDEAAALVFEGDAASDKFIGIRLGAAGQNSAEALIR
                     LGRLDEADDLLRRLARGTGNCVFGPFAVRSIIAMRRGLLDDAVALLVAGEETSGGLDT
                     VNVQGWLHTLRAEIYLERGEPRAAMEQVDRALTTAVGAEDDTLVIE"
BASE COUNT          350 a          678 c          652 g          330 t
ORIGIN      
        1 gctgaggttg cgggggtact cggcttcgcg cagcggtccc tcggactcgt ctctccaacg
       61 gcctcaacca ccgacgtgtc cgacgcgcag ttacccccgg cgactgggct ggtcaagacg
      121 aagctgttcg agtcgctgct ccagagcatc gaaaccctga cgcagcgggc cccggtcgtg
      181 ctggtcttcg aagacctgca atgggccgac tccgcaagca ttgaagtgtt cgacttcctt
      241 acccgaaacc tcggtgaccg cccggtactt ctggtggcga cctaccgatc cgacgaggtc
      301 gatcgaggtg gcgcccttcg ccgcctgctc gccgaactcg gcagacacag ccgcgtctcg
      361 ctgcttgaac tgtcaggtct cgaccgggtc gagattgcga gcctgctcac cgacattgtc
      421 ggcgggacgc ctgattggac gctcgtcgac gccgtcctga cacgttcggg cggcaacccg
      481 ttcttcgcgg aggaactggc ggcggcacgc cacgccaagg cgctcccgac ggccctgcga
      541 aacgtcgtca tgatgcgaat cgagcgcctc tcgccacacg ccttgcatgt cgtgggggtt
      601 gccgccgcgg ccggcacgtc gattcatcac cgattgctgt ccgcaaccgc gcgcatcgat
      661 gccgacgagc tcgacgccgc ggtgtcggaa gcaatagatc acaaggtgct cgtggcagac
      721 gcagacacgg gttcgttccg attccgccac gcactcctgc gggaggcggc ctacgacgcg
      781 ctgctgccag gcgagcgagc ccgcttgcat cgagcgctcg ccggcgcatt gacagcccac
      841 cccgaactcg gcgctgccgg tcccggctac gcagcaatgg agttggccga ccattggtgg
      901 gaggccgcgg attggcccga ggcgctacgc gcatccgtta atgccgccga cgctgcggcc
      961 ggggtcttcg caatgcctga ggcgtacgcg tacttcgagc gcgccctcgc agcgggtgac
     1021 ctcaccccga ctgactcgca agtatgggtc ggcatcgacc gcgtcgacct cggtgtgaaa
     1081 accgcagatg ccgcctacat gagcggtgaa acccagcgat cagtcgacct cgtacgacgt
     1141 gctcttgagg ccttggacgc cggcactgac ccgcagcgag cggccatctg ctacacgatg
     1201 ctggggcgca acgcatgggc gatcggtgac tctgcgctcg cgctcgattc cttgcacgca
     1261 gcggcagcgc tcttgcccgc cgatgctccg tcgcttgagt tggcgggaat ccttgctgag
     1321 gaagctcgga gcctgatgct catgtcgcac atggaccgag ccgaggcgaa ggcgcacgag
     1381 gccatcgcaa cagcccgcgc cgtcggtgcc cgcagggagg aaggacacgc cctcaacacg
     1441 ctgggctgca ttgtcagcga ccgaggcgac aacacccggg cgctagtgct gatgcgcgaa
     1501 tcgctggcca tcgccgaaga actaggcaac cccgaccagc tttaccgcgg atacaacaat
     1561 ctcgcgcacg tcctgagtca agcagggcaa ctcgacgaag ccgccgcgtt ggtcttcgaa
     1621 ggcgatgccg cgagcgacaa attcatcggc atccgactcg gtgcggccgg tcagaacagc
     1681 gcagaggcgc tcatccgact cggccgtttg gacgaggccg acgacctgct gcgacgactt
     1741 gcccgcggaa caggcaactg cgtgttcggg cctttcgcag ttcggtccat catcgcgatg
     1801 cgccgcggac tgctcgacga cgccgttgcc ttgctggtag cgggcgagga gacgtcaggc
     1861 ggactcgaca ccgtgaacgt gcaggggtgg ctgcacacgt tgcgcgcgga gatctacctt
     1921 gaacgcggcg agccgcgcgc cgcgatggaa caggtcgatc gcgcgcttac gaccgccgtc
     1981 ggcgccgaag acgacacgct ggtaatcgag
//
LOCUS       DASWVF010000536         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_422235, whole genome shotgun sequence.
ACCESSION   DASWVF010000536 DASWVF010000000
VERSION     DASWVF010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422235"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGD55_09475"
     CDS             <1..220
                     /locus_tag="VGD55_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870616.1"
                     /translation="SISRMFRVHRVTVARWLWRAGDLIQNGLRQTFRDRLGVASQEFD
                     SLARRLPSRLDYDLSSALEPSVKVAAGD"
     gene            complement(230..2377)
                     /locus_tag="VGD55_09480"
     CDS             complement(230..2377)
                     /locus_tag="VGD55_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870617.1"
                     /translation="MTGNGLADIVAVNSDGINMRRSGRWFFATKVETLSTLAPSASAT
                     RLMADLTGDKRADYVELSPTAPVTVRVRQNRRIPIRFVQLMSPTSDCGSRSSDATILE
                     ALADANLKYQDVGLEFYARPSTSYPTQCPVLSLSTTVVAQALSSLGGSASQAELANVR
                     DALNPACDMGAGDFSSASGSDQLWWLSARCAVAGEIVGFVTDNPAGHFSGDAAKGKML
                     VLHGVDTQLAHEIGHYLGLPHNFEVGLPYGMDPLTGQDDPTSAFWDLAFQPSQSKGPD
                     LFFNSRAEAAVYEPQLTRIQIGTYVPGDPTFQSVDGWTCPNAFGSPCPSPSLPVGSVA
                     MPLAGNTFQLLYTFYPSSNPPQLDPRMKGISPHPADGLIDPNTQVPLPGINVMSYNYG
                     GAQGAPGEQAWFSLSQIEFIQNALTFDVAVSKLDATGHVIFGDRPRLGIDVPPASLQT
                     VPTGGGVMPGPGTLASNGAMTWVVGGNPYSADGNQVYQWNPHTRVWDPIAAGANQVTL
                     PPGSPTAWAVTGSGSRVKRWTGSSFAVLPADPAGTCVRSLVVGVHSSGTYAWSRSCQS
                     ADGSGNYPIIRYDDTNGWTSIAGPEPGASVNGAFDLAVDWQGSVWALANRAGQPAVYR
                     ANLGSNGLPANWTRYAAPAGAVHITGGADIHGNDHIPVNILDSSQGVSIFNNEGSSWS
                     PAMTLPFSVRQVSGALPYLWVVNDADGSIWYVR"
     gene            complement(2572..3902)
                     /locus_tag="VGD55_09485"
                     /pseudo
     CDS             complement(2572..3902)
                     /locus_tag="VGD55_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          683 a         1254 c         1304 g          733 t
ORIGIN      
        1 gtccatctcg cgtatgtttc gggtgcatcg ggtcactgtt gcacgatggc tctggcgcgc
       61 cggagacctg atccagaacg gcctgcggca gacgtttcgg gaccggctgg gcgtcgcctc
      121 gcaagagttc gacagccttg caaggcgact ccccagcagg ctcgactatg acctatcgtc
      181 ggcgctggag ccctcagtca aggtcgctgc cggcgattga ccgctcggct cagcggacat
      241 accaaatact cccgtctgca tcgttgacca cccagagata gggcagcgct cccgagacct
      301 gcctgacact aaacggcagg gtcatggctg gtgaccacga cgagccctcg ttgttgaaga
      361 tcgacacgcc ttgcgacgag tcgagaatgt tgacggggat gtggtcgttg ccgtgaatgt
      421 ccgcaccacc ggtgatgtga acggcgccgg ccggcgctgc gtagcgcgtc cagttggcgg
      481 ggaggccgtt cgatccgaga tttgccctgt acaccgcggg ctgccctgcc ctgttcgcga
      541 gcgcccacac agaaccctgc cagtccaccg ccaggtcgaa cgcgccgttg acggatgcgc
      601 ccggctcggg acccgcgatg gacgtccatc cgttcgtgtc gtcgtaccga atgatcgggt
      661 agttaccgga gccgtccgcg ctctggcagc tccgggacca ggcgtatgtc cccgacgagt
      721 ggacccccac caccaacgac ctgacgcagg ttccggccgg atcggctgga aggacggcga
      781 acgatgagcc cgtccagcgc ttcacccgcg acccgctccc ggtgaccgcc caggcggtcg
      841 gcgacccggg cggcagcgtc acctggttcg cgccggcggc tatcggatcc cacacccgcg
      901 tgtgggggtt ccactggtac acctggttgc cgtcggcgga ataaggattg cccccaacga
      961 cccaggtcat cgcaccgttc gaagccaggg ttccgggccc cggcatgacg ccgccgccgg
     1021 tgggcacggt ttgcagcgac gccggcggca catcaattcc tagtcgggga cgatcaccga
     1081 agatcacgtg cccggtcgcg tcgagtttgc tgaccgcaac gtcgaacgtc agcgcattct
     1141 gaatgaattc gatttgcgaa aggctgaacc atgcttgttc gcccggtgca ccttgcgcgc
     1201 ctccgtagtt gtacgacatg acgttgatcc caggcagcgg cacctgcgta ttgggatcga
     1261 tgagaccgtc cgctggatgt gggcttatgc ccttcatcct gggatccagc tgggggggat
     1321 tgctggaagg atagaacgtg tacagaagct gaaacgtgtt cccggccagc ggcatcgcga
     1381 ccgagccaac cggcaaagaa ggactggggc acgggctccc aaacgcattc ggacacgtcc
     1441 acccgtccac tgattgaaag gtcgggtcgc cgggcacata agtcccgatc tggattcgag
     1501 tcagctgcgg ttcgtagaca gccgcttccg ctcggctgtt gaagaacagg tccggtcctt
     1561 tcgattggga aggctggaag gccaagtccc aaaacgccga cgtcggatcg tcctgtcctg
     1621 tcagcggatc cattccgtag ggcagcccca cctcaaagtt gtgcggcaga ccgaggtagt
     1681 gaccgatctc gtgggcaagc tgggtgtcca ccccatgcag caccagcatt ttgcccttgg
     1741 cggcgtcccc tgaaaagtgc ccggcagggt tgtccgtgac aaaacccacg atttcgccgg
     1801 ccaccgcgca gcgcgccgac aaccaccaca actgatccga cccactcgcg gaactgaaat
     1861 cgcccgcacc catgtcgcag gcgggattca aagcgtctcg aacattcgcg agctccgcct
     1921 gcgatgccga tcctcccaac gacgagagcg cttgagcaac cacggtggtg ctcaggctca
     1981 gaaccgggca ttgggttgga tacgacgtgc tcggtctggc atagaattcc agaccgacgt
     2041 cctgatactt gaggtttgca tcagcgaggg cttccaggat agtcgcatcc gacgatcgtg
     2101 agccgcagtc gctcgtcgga gacatgagct ggacgaaacg aatcgggatc ctccggtttt
     2161 gccgaacccg cactgtcacc ggggcggtcg gagacaactc aacataatcg gcccgtttgt
     2221 cgccggtgag gtcagccatg agccgggtcg cggacgcaga cggcgccagc gttgacagcg
     2281 tttcgacttt ggtggcgaag aaccatcgcc ccgaacgccg catgttgatg ccgtcgctat
     2341 tcaccgccac gatgtccgcc agaccgttgc ccgtcacgtc ggtcaggaaa gtatcgacgt
     2401 ttccgtagaa cgctgtcgtc gaccaggtct cgggtgagcc caggccaagg cttggccgga
     2461 acctgtcaac gagaatgaga cagcggtggc tcgagtttag tgaatgtcct cggcgtcgat
     2521 cgacgcctgc gttggtgggt tgatccagac agcggtgggt ggtggcggtg gcgtgggcac
     2581 gccagcaggg aagcgctcag gatgcgcgac gtgtgcggcg acgaggacgc gagctcgcgc
     2641 tgcgacgcgc tcgtcggcga ggccgaagtg gacgtcgtgc ggggtcagga ggccgatgcc
     2701 accgtggcga tgctcgcggt tgtaccagtc gaagaacggg ccgcagtgcg cccggccgtg
     2761 ctgggcggag ccgaagcgat cgggaaagtt gggccggtac ttcagcgtct tgaactgcgc
     2821 ctcggagaac gggttgtcgt tggagacgtt cggcctcgag tggctctgcg tgacgccgag
     2881 gtcggcgtac agatgcgcga tcgtcttcga cgtcatcgac ggacctcgat ccgcgtggat
     2941 tgcgagttgg cccggctcga tgccgtgtcg ccgacaggac tcttcgatca gcttcttggc
     3001 aagggtcgcg ctttcgcggt gcgcgatcat ccagccgacg acgtagcggc tgaagatgtc
     3061 gagcacgacg tacaagtaga ggtaggtcca cttcgcgggg ccgaggagct tcgtgatgtc
     3121 ccagctccac acctggttcg gcgccgtcgc cagcaactcg ggcttcttgt acgtcgggtg
     3181 ccgcagttga tcgcgccgct cacggacctc ggcattttca gcgaggatcc ggtacatcgt
     3241 ccgctccgag cagaggtaac gattctcgtc aagaagccga gcgtagacct cggccggcgc
     3301 ctggtcggcg aagcgcggct cgtgcaggac gtcgagcacg gttcgtctct cggcgacggt
     3361 gagggcccga gtcggcgtcg gccgtggtgg cggcgcattg gcctcgaggt gccggtagta
     3421 cgtcgcccgc ggcacagcca gcgcgcggca caggcggccg acgccgagtc tcgaaccgtg
     3481 gagcgcgacc gtctccatca cgattcctcg ctcgggaacg gacgtcccag cagtgcggcc
     3541 actttttttt gaatctcagc gatggcctcg gcgcgctcgg cacgcgtcgt cagcttggcg
     3601 atctggcgtt ccatgtcggc gatgcgtttg tcacgcgggt cgggtgccgt cgtcgccggc
     3661 ccgcgcttcc ttgccttggc tccgggggcc aactcgcctc gatcccgtgc ttgccgccac
     3721 gtcgtcaggt gcgacgagta gaggccttca cgccgaagca gcgcaccgat gtcgccggga
     3781 cctttgcagg cctcggcctc cttcacgatc tgcagcttgt actcgacggt gtacgtccgt
     3841 cgcctagcct tctcgggcac ctcggtcacc tcgccgaggg acgttatcgc ggtcgcggtc
     3901 atggtgggat ccttgctcgc cctgctcagt aaacttcagg ggtcgagctg tctcactcac
     3961 gttggcagag aggg
//
LOCUS       DASWVF010000537         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_447960, whole genome shotgun sequence.
ACCESSION   DASWVF010000537 DASWVF010000000
VERSION     DASWVF010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447960"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VGD55_09490"
     CDS             <1..1367
                     /locus_tag="VGD55_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870618.1"
                     /translation="SEPGQFITWGYYDQYVTDELCEFLRIDGVPDGTYDLVVEENTDR
                     LINCDGTESSCHFDGRLDDNVVAMRITIAGSQVSFPAPSLEGGATGLGPFEMLGAPAV
                     VTHAIDTYDLYYVASDHGLYTLHQNASGVWTGAGGGSSDAGSLVFNPSAAGYPMTGTI
                     AAVVVDNRALATPTIRTDVFGGATTGDLLRFSITGSGLQVFQYGVAGQGYGIAGTAAV
                     VDSSAKNAIAVWTTPQGAVAFGSYSGTDWLPPRLVSAPFVAASQTPGLTSSGDGMFHL
                     FIKDSSGNAWYDQYVNGAWGTWQQLSTGAVAPWQGNLAAVSSYMRSADVFATTTDHRV
                     FRNTWTETDAGQGFSGWSDPLAGNNADRRVGSSLTAVSTGTRKKDVFFYDAISPTMLW
                     QLHYDETVTGPQPPALPWTLRNLSKILVVQPMWPVVVGSWADNTFDLFQKFTDARIGV
                     RRQR"
     gene            1438..1707
                     /locus_tag="VGD55_09495"
     CDS             1438..1707
                     /locus_tag="VGD55_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870619.1"
                     /translation="MNPGSRVSGAGAVLALLPLVGTTGCKGVVDPIPSAFAASEQRAY
                     HGGLLGRFVEEVGATDPGCDPGSVMGYFDGNTVTALWNYARSTTP"
     gene            complement(1697..>1938)
                     /locus_tag="VGD55_09500"
     CDS             complement(1697..>1938)
                     /locus_tag="VGD55_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870620.1"
                     /translation="TSHVAAPRSPHPHISIPTRVGRAAGGNFRGHDVGDVHGVSSVPS
                     TTAKWAPRHSLPVAALRPADIEGPNVDPKKLSLKA"
BASE COUNT          316 a          630 c          645 g          347 t
ORIGIN      
        1 catcggagcc ggggcaattc atcacctggg gctattacga ccagtatgta accgacgagc
       61 tctgtgagtt cctgcgtatc gatggggtcc ccgacggcac ctacgatctt gtcgtggagg
      121 aaaacaccga ccgtctcatc aattgcgacg gcaccgagag cagttgccac ttcgacggcc
      181 ggctggacga caacgtcgtc gcgatgcgca tcaccatcgc aggcagccag gtgtcttttc
      241 ctgctccttc cctggagggc ggggccacag gactcggccc ttttgagatg ctgggagcgc
      301 cggcggtcgt gacgcacgcg atcgacacct acgacctcta ctacgtggct tcggaccatg
      361 gcctttatac ccttcatcag aatgcgagcg gcgtctggac cggggcgggg ggcggctcca
      421 gcgatgctgg ttcgctggtg ttcaacccgt ccgccgccgg gtacccgatg acggggacga
      481 ttgccgccgt ggtcgtcgac aaccgcgcgc tcgcgacgcc cacgatccgg accgacgtct
      541 ttggcggagc gacgacgggc gatctgctcc gcttctcgat taccggcagc ggtctgcagg
      601 tcttccagta cggggtcgca ggccaggggt acgggatcgc cggaaccgcg gcggtggtgg
      661 attcgagcgc gaagaacgcg attgccgtgt ggacgacgcc gcaaggcgca gtggcgttcg
      721 gctcctacag cggaacggat tggctcccgc cgcggctggt gtcggcccca ttcgtcgcgg
      781 ccagccaaac gccggggctc acgtcgtccg gcgacggcat gtttcacctg ttcatcaaag
      841 acagctcggg aaacgcctgg tacgaccagt acgtgaacgg cgcctgggga acctggcaac
      901 agctctcgac cggcgctgtc gcgccctggc aggggaacct ggccgctgtg tcctcataca
      961 tgaggtcggc cgacgtgttc gcgaccacga ccgaccaccg ggtgttccga aacacctgga
     1021 ccgagacgga tgcgggacag gggttctccg gctggtcgga tccgcttgcc gggaacaacg
     1081 ccgacaggcg cgtgggttcg tcattgacgg ccgtcagcac gggaacgcga aagaaggacg
     1141 tgttcttcta cgacgcgatc agtcccacga tgctgtggca gctccattac gacgagacgg
     1201 tgaccggtcc ccagcccccc gctctgccct ggacgctcag gaatctgagc aagatccttg
     1261 tcgtgcagcc catgtggccc gtcgtcgtcg ggtcctgggc cgacaacacc ttcgatctct
     1321 tccagaagtt cacggacgcc cggatcgggg tgcgccggca gcgctagctt cggcacgctt
     1381 gacgcccttt gccgcttgtt ctcctaaccc gcgcatgccg agaagcaccc cgcgcgggtg
     1441 aaccccggct ctcgagtttc gggcgccggc gctgtgctgg cgttgctgcc gctggtcggc
     1501 acgaccggtt gcaagggcgt cgtcgatcca attccgtcgg cgttcgcggc ctccgagcaa
     1561 cgcgcctatc atggcggcct tctcggccga ttcgtcgagg aggtgggcgc gaccgacccg
     1621 ggctgcgatc cagggtccgt gatgggctac ttcgacggga acaccgtcac cgcgctctgg
     1681 aattacgcgc gcagcactac gccttgagcg acaacttctt cgggtcgacg ttcggtccct
     1741 cgatgtctgc cggccgcaag gcggccacgg gcaggctgtg gcgcggcgcc cactttgcag
     1801 tcgtggaggg aacggaggac acgccatgaa cgtcgcccac gtcatgacca cggaagttgc
     1861 cacctgccgc tcgaccgact cgcgtgggaa tcgagatgtg gggatgggga cttctcggcg
     1921 cggcgacgtg gctggtct
//
LOCUS       DASWVF010000538         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_371220, whole genome shotgun sequence.
ACCESSION   DASWVF010000538 DASWVF010000000
VERSION     DASWVF010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371220"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGD55_09505"
     CDS             <1..624
                     /locus_tag="VGD55_09505"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY0870621.1"
                     /translation="HRVPYRANLWALRAVGVRQVLAPCAVGSLRADIAPGSLVVPDQV
                     VDRTWGREHTFYDVPGSVVHVGFADPYCPNGRHTVVTVAAGQDWAPRDGGTLVVIQGP
                     RFSTRAESEHHTAQGWTVVGMTGQPEATLARELALCYTSIAIVTDLDAGVEVGQGVTH
                     LEVLDAFAASIARLRSLLSGAIAALPTERTCECSHALDGQTLPFALP"
     gene            624..1436
                     /locus_tag="VGD55_09510"
     CDS             624..1436
                     /locus_tag="VGD55_09510"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY0870622.1"
                     /translation="MRRRRDAGAALAWVPLRPTPPPLRDAPSLRPPRRTVEHVWREIR
                     IGARRYRQLLRAALVTIALALVVARLAPKPPATIAVVAAARDLPIGEVLTAADLHTVN
                     LPPATVPSGVATDVGAVVGSQLSGPLRRNEPLTDVRLGGGVLRRPGSGLVSAPVRLAD
                     SQAALLLQPGQRIDVLAANTDTGGGSAPVAGAGNPAGVAGPVVGAAATVVAADVMVVA
                     IPIASAPPGATSDATGDVGAEGTLVVLATTVIQARQLAQAQVSSRLSAVVVG"
     gene            1542..1946
                     /gene="mscL"
                     /locus_tag="VGD55_09515"
     CDS             1542..1946
                     /gene="mscL"
                     /locus_tag="VGD55_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0870623.1"
                     /translation="MSGFKKFLLRGNLVDLAVAFVIGAAFAALVTSLVKDFITPLLGL
                     IGGKGAFGGRTFTWGHSVFAYGDFINAVIAFIIMAAVVYYLVVVPYGALLDRYKKEPE
                     PDAPVRECPECLSSIPAAATRCAFCTAVSAAA"
     gene            complement(2045..2176)
                     /locus_tag="VGD55_09520"
     CDS             complement(2045..2176)
                     /locus_tag="VGD55_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870624.1"
                     /translation="MSESIPEQTADDTDAGWNDESAEEKAERDAEWYARETPPHHGD"
     gene            complement(2251..2541)
                     /locus_tag="VGD55_09525"
                     /pseudo
     CDS             complement(2251..2541)
                     /locus_tag="VGD55_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
     gene            2643..3494
                     /gene="purU"
                     /locus_tag="VGD55_09530"
     CDS             2643..3494
                     /gene="purU"
                     /locus_tag="VGD55_09530"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY0870625.1"
                     /translation="MDGFILTLSCPDRVGIVHAVTGFLSAHRCNIVDSQQFGDRDTGR
                     FFMRVHLEGNDEPRSLDELRDAFAAVGETFAMDWHIDGETARARIIIMVSQQGHCLND
                     LLYRWRIGSLPGDIAAIVSNHRDFEVLAKSYGVPFHYVPVTTSTRDDAEQRLLDIVAE
                     TGAELVILARYMQVLSARVCEQLPGRIINIHHSFLPSFKGAKPYHQAHDRGVKLIGAT
                     AHYVTANLDDGPIIEQEVARVDHGDDVENLTSIGRDVECLALARAVRWHLEHRVLLDG
                     HKTIVFR"
BASE COUNT          532 a         1248 c         1204 g          609 t
ORIGIN      
        1 caccgggtgc cctaccgggc gaatctgtgg gcgctgcggg ctgtcggcgt ccgccaggtg
       61 cttgcgccgt gcgccgtcgg ctcgctgcgc gctgacatcg cgccgggcag tctcgtcgtc
      121 cccgatcaag tcgtcgaccg cacgtggggc cgcgagcaca cgttctacga cgtccccggc
      181 agtgtcgtgc acgtcgggtt cgccgacccg tactgcccga acggacggca caccgtcgtc
      241 actgtcgcgg ccggccaaga ctgggcgccg cgcgatggcg gcaccctcgt cgtgatccag
      301 ggcccacgat tttccactcg agcggaatct gagcatcaca ctgcccaagg ctggaccgtg
      361 gtcggcatga ccggtcagcc cgaagcgacc ctcgcccgcg aactggcgct ttgctacacc
      421 tcgatcgcga tcgtgactga tctcgacgcc ggcgtcgagg tcgggcaagg cgtcacgcat
      481 ctagaggtgc tcgatgcgtt cgcggcgagc attgcgcggc tgcggtcgct gctgagcggt
      541 gcgatcgcgg cgctgccgac tgagcgaacc tgtgagtgct cgcacgcgct cgatggccag
      601 acgctgcctt tcgccctgcc gtgatgaggc ggcggcgcga cgccggcgct gcactcgcct
      661 gggtccccct tcgacccacg ccgccgcctc ttcgcgacgc gccatcgctg cgccctccgc
      721 ggcgaaccgt cgagcacgtg tggcgcgaga tccggatcgg cgcgcgtcgc taccggcagt
      781 tgctccgcgc cgcgctcgtg acgatcgcgc tcgcccttgt cgtcgcacgg ctggcaccca
      841 agcccccggc gacgatcgcg gtcgtggctg cggctcgcga tctgccgatc ggagaggtgc
      901 tgaccgcggc cgacctgcac acggtgaacc tgcctccggc gaccgtccca tcgggggtcg
      961 cgactgacgt gggcgcggtc gtcgggagcc agttgtcggg gccgctgcga cgaaatgagc
     1021 ctctaaccga cgtccggctg ggcggtggcg ttctccggcg gcccgggtcc ggactcgtct
     1081 cggcaccggt gcggctcgcc gactcccaag ctgccctgct gctgcaaccg ggccagcgca
     1141 tcgacgtcct ggcggcaaac actgacaccg gcggtggatc ggcccctgtc gccggcgctg
     1201 gtaacccggc aggcgtcgcg gggccagttg tcggcgccgc cgcgaccgtc gtcgccgcgg
     1261 acgtgatggt cgttgcgatc ccgatcgcgt cggcaccgcc gggggcgacg tccgacgcaa
     1321 ccggtgacgt cggcgccgag ggcaccctcg tcgtgctcgc cacgactgtg attcaggcga
     1381 ggcagctcgc ccaggcgcag gtcagctcgc gactgtcggc agttgtcgtc ggctgagcag
     1441 gggtgcgcaa atcggccata gagcgctatg ttccggtgcc gcttcgatgt gtctggcatc
     1501 gacggtggtc cggacccgag ccccgacaga gggagaacac agtgtcaggc ttcaagaagt
     1561 tcctgctgcg cggaaacctg gtcgacctcg cggtcgcgtt cgtcatcggc gccgcgttcg
     1621 ctgcactggt gacgtccctc gtcaaggact tcatcacccc gttgctcgga ctcatcggcg
     1681 gcaagggagc gttcggcggc cgcaccttca cctggggcca cagcgtcttc gcctacggcg
     1741 acttcatcaa cgccgtgatc gcgttcatca tcatggctgc ggtcgtttac tacctcgtcg
     1801 tcgtgcccta cggagcgctg ctcgaccgct acaagaagga gccggagccc gacgcgccgg
     1861 ttcgcgagtg ccccgagtgc ctgagcagca tccccgccgc ggccacccgg tgcgcgttct
     1921 gcacggcggt atcagcagcc gcgtagtcct gggcaacaac gtggagtgct gagcgtgttc
     1981 tagcgacgtc cagactccac gttgtcccgt cggcgaggac cgtcggcgag gaccgtcggc
     2041 gaggtcagtc gccgtggtgc gggggcgtct cgcgggcgta ccactcggcg tcccgctcgg
     2101 ccttctcttc ggcgctctcg tcgttccacc ccgcgtcggt gtcgtcggcc gtctgctcgg
     2161 ggattgactc gctcatcggt cgcccgccag ggcagattcg gcattcgagg cggtgccggc
     2221 cgaggctgcc gagggcgtct cagccgcggc gtcgaggttc aacacccgca cgtgcaacac
     2281 gctgcgctgc tgcaaggccg cccgcagcgc gcggtgcagg ccgtcctcga ggtagagctc
     2341 gtcgtgccac tgcacgacgt gcgggaacag gtcgccataa aacgtcgagt cgtcatcgag
     2401 caggacgtcg agggcgagca ccttcttggt cgtgaccaac tggtcgagcc ggacctgccg
     2461 gggcggcacc gcggcccaat cgcgcagcat caggtcgtgc gcagggtagg gccggccctc
     2521 ctgaacggtc ttgaagatca cgaggtcccc ctatccgtcg gccgtgtaag ccatgcaagc
     2581 cgtgcgaccc gcaggcacta ttctgcgtca aacgaagaca acagcgagag ggtgcccaca
     2641 tcgtggacgg cttcatcctc acgctctcat gccccgaccg ggtcggcatc gtgcacgcgg
     2701 tcaccggatt cttgtccgct caccggtgca acatcgtcga cagtcagcag ttcggcgacc
     2761 gcgatacggg ccgcttcttc atgcgcgtgc atctcgaggg taacgacgaa ccgcgcagcc
     2821 tcgacgagtt gcgagatgcg ttcgcggcag tgggtgagac cttcgcgatg gactggcaca
     2881 tcgacggcga gacggcccgc gcccgcatca tcatcatggt gtcgcagcag gggcattgcc
     2941 tgaacgactt gctgtaccgc tggcgcatcg gctcattgcc cggcgatatc gcggccatcg
     3001 tctcgaacca tcgcgacttc gaggtgctcg cgaagtcgta cggcgttccg ttccactacg
     3061 taccggtcac cacttcgacg cgggacgacg cggagcagcg gttacttgac atcgtcgccg
     3121 aaaccggcgc tgagctagtg attctcgccc gctacatgca ggtgctgtcg gcgcgggtgt
     3181 gcgagcaact gccgggacgc atcatcaaca tccatcactc gttcttgccg agcttcaaag
     3241 gcgccaagcc ctaccaccag gcccacgatc gcggagtgaa gctgatcggc gcgaccgcgc
     3301 actacgtgac ggccaacctc gacgacggtc cgatcatcga gcaagaggtc gcgcgggtcg
     3361 atcacggcga cgatgtcgag aacctcacct cgatcgggcg ggacgtcgag tgcctagccc
     3421 tcgcgcgcgc agttcgctgg cacctcgagc accgcgtgct cctcgacggc cacaagacga
     3481 tcgtcttccg ctagcgccgc aacagcccgt gcatctgccg ccctcggagg gcggtgatcg
     3541 cactggttgt ggctgatggg cgtgcgacag tggtcggcat ggcggcgcac atc
//
LOCUS       DASWVF010000539         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_39923, whole genome shotgun sequence.
ACCESSION   DASWVF010000539 DASWVF010000000
VERSION     DASWVF010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39923"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGD55_09535"
     CDS             complement(<1..409)
                     /locus_tag="VGD55_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870626.1"
                     /translation="MRLDRAATKLLALASTVGVVAAITVTSAAPAGASTAASFGPRLQ
                     AIVALYGDSAISVPGLHVQSLLGSIHSEIVDGTAAQLAQLATTPGVLGVEPNVAAHLE
                     SDSFGAKSTSSGHDAGGDSNGDNSGSNAPSPSSP"
     gene            871..1488
                     /locus_tag="VGD55_09540"
     CDS             871..1488
                     /locus_tag="VGD55_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870627.1"
                     /translation="MVVDDHPLVRESMVARLTAMGAREIVEAASCAEARARARASGPR
                     DLCVVDVGLPDGSGLDLLAELRSAGWPRLVVLSAADDPYSVRAAFVAGAQGYLLKSAS
                     PVVVADGVRRVLDGGVYADPSVASLLAAGLRNGPTNEGVGELSGREVEVLRLVADGRS
                     NKEIGDELGLSALTVKSHLARIARKLGTGDRAEMVALAMRAGVIR"
     gene            1597..2997
                     /locus_tag="VGD55_09545"
     CDS             1597..2997
                     /locus_tag="VGD55_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY0870628.1"
                     /translation="MSESAVSAVFLEPVMPPEESSAPAGVPPTDTAVEPETVELAPLL
                     QVRDGVPHIIDDDETLTAAVARLAAGTGPVAVDAERASSYRYGHRAYLVQLRRVGAGI
                     LLIDPIACPDLSSVSAALGDAEWVVHAANQDLPCLADLGMYPPSLFDTELAGRIAGFP
                     RVGLATMVENLLGWRMEKDHSAADWSSRPLPEPWLRYAALDVELLIELRDALESALDR
                     QGKLAWAHEEFEHVRTAPPAAISVERWRRLSGIHKIRHRRQLAIVRSMWVTRDRIARE
                     TDTSPSRLLPDASILQVALETPANARELAGSADFHGRGSRRYLAQWWAAIADALAEDV
                     DGLPEMTRPSEGPPPGHRWAERDRAAADRLAAAKAVVAALADEHGLPAENLLQPDAVR
                     RLAWRPPSDLFAGPVGDDLRGFGAREWQIALTAGPLAHALLRAATHEGTHESAHEAGD
                     PSIEASNEPGQTVATD"
     gene            3074..3943
                     /locus_tag="VGD55_09550"
     CDS             3074..3943
                     /locus_tag="VGD55_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870629.1"
                     /translation="MSMPVGGPNREERTALRRCMLAVSVLDDVDMLPTDDGMVLSGVP
                     QLTVTFADIAAAIGATDPAGPTARHRLELWLRARRAIADKSLDELAETIRPVGLPVGH
                     DVHPGPSWVRQPILGGCLDLGVGFVGLFRDVPDAVVVTPQSVLDAAGIDPGPWWRGAT
                     EYLENMGALATTRWRRDPRSPVRPMGDCDVITLLASSLFRGALCADAGGMRAIAAPVR
                     TRGWLDLSRIDPAFAQTAAALADDESRGFPRPLLLTVDEVSMVSEGGRPAEIVLRDPA
                     TDEKRWLRDVLYH"
     gene            complement(4083..>4575)
                     /locus_tag="VGD55_09555"
     CDS             complement(4083..>4575)
                     /locus_tag="VGD55_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0870630.1"
                     /translation="IRFPKGALPRDIERIDRIGGLDVLRRDEAADVLIVAFGPMAALA
                     LEVSGRLADQGIASTVVDPRWVLPVNPAIAGLAASHSLVVSIEDHGRQGGAGSTVSMA
                     LRDAGVSTPVQVQSIPQRFLAQGKRAEILSEIGLTAQEIARQVVESVAGIDALDLTQP
                     VTD"
BASE COUNT          716 a         1595 c         1509 g          755 t
ORIGIN      
        1 tcggactcga aggagagggg gcgttcgatc cgctgttgtc accgttcgag tcgccgccgg
       61 cgtcgtgacc actggaggtc gacttggcgc cgaacgagtc gctctcgagg tgcgccgcga
      121 cgttgggctc gacgcccagg acgccggggg tcgtcgcgag ctgggccagc tgggccgcgg
      181 tcccgtcgac gatctcgcta tgtatggagc cgagcagcga ctgcacgtgc agtcctggca
      241 cggaaatggc cgagtcgccg tagagcgcaa caatggcctg aaggcgaggt ccgaaactgg
      301 ccgccgtcga ggcacctgcc ggcgctgctg acgtgaccgt gatggcagct acaaccccca
      361 cggtggatgc aagagcaagc aacttcgtgg ccgctcgatc cagtctcacc aatatctcct
      421 cgcgatgcgc aggtctagcc cacgtgctgc ccacgtgctt tctcggcaaa gcacgcctag
      481 atctgttgtc cgggagccag agaccgccca cttggcgtag tgattcgcgc tagtccggtg
      541 atggtgttct ctgggtattt agagtcatct gctcggccct acctggcgtc tggccgagct
      601 acaacggcgg gaccgcgctc accgagtgat ctatggagta gggcgatctg agccgacggc
      661 gcgagaatcg agcgcttcta ccgcaaacgg actaatccga agcttcagcc gcgcctcgcg
      721 ccgtccgatg cattggacga gagatgaccg cagaccgtgg tcatcgagca gtgcggccgc
      781 ggaagcggcc ctgacgctag gaggaccggg gtgacggcgt tgtttgagca cgtgccgccc
      841 cagccggaga actccgcggg cttcaccgcc atggtggtcg acgatcaccc cctggtccgc
      901 gagtcgatgg tcgcccgcct caccgcgatg ggagctcgcg aaatcgtcga ggctgccagc
      961 tgcgcggaag cgcgtgcccg agcccgagcc agcggtcccc gagacttgtg tgtcgttgac
     1021 gtcggcctgc ccgacggttc cggccttgat ctgctcgccg agctccgcag cgcgggctgg
     1081 cctcgccttg tcgtgctctc ggcggctgac gatccctact ccgtacgcgc cgcgttcgtt
     1141 gccggcgccc agggctacct gctgaagtct gcgtcacctg tggtcgttgc cgacggcgtc
     1201 cgccgggtgc tcgacggcgg cgtttacgcc gatccttcgg tggcttctct tcttgcagcc
     1261 ggtttgcgca acggcccgac caacgaaggc gtcggcgaac tgtcgggccg cgaggtcgag
     1321 gttctgcgat tggttgccga cggccgctcc aacaaagaga tcggcgacga gctcggtctc
     1381 tcggcgctga ccgtcaagag ccacctcgcc cgcatcgcac gcaagcttgg aacgggtgac
     1441 cgcgccgaga tggtcgcgct ggccatgcgc gccggcgtta tccgctagtc cttaccactg
     1501 agctcagggg cggtgacggg ccatctcggc ctgtcgctgg cctgtcgctg gcctgtccgt
     1561 gcgcctccgg cgcactcctg gtcgtacctt ggtgacatgt cggaatcggc tgtctcagcg
     1621 gtcttcctcg agcctgtgat gccgcccgaa gaatcttcgg cccccgcggg cgtgccccca
     1681 acggacaccg ccgtcgaacc cgagaccgtc gaactcgctc ccctgcttca ggtacgcgac
     1741 ggcgtcccgc acatcatcga cgacgatgag acgttgactg ccgccgtcgc gcgattggcc
     1801 gccggcaccg gcccggtcgc cgtcgatgca gagcgtgcct cgagttaccg ctacgggcat
     1861 cgcgcctatc tggtccaact ccgacgcgtg ggcgccggca tcttgctcat cgacccgatc
     1921 gcctgccccg acctgtcgag cgtgagcgcg gctctgggcg atgccgaatg ggtcgtccac
     1981 gctgcgaacc aggacctccc gtgtcttgcc gatctcggga tgtacccgcc gagcctgttc
     2041 gacaccgaac tcgccgggcg gatcgccgga tttccccgcg tgggcctcgc cacgatggtc
     2101 gagaaccttc tcggttggcg gatggagaag gatcactcgg ctgctgactg gtcgagccgt
     2161 ccgttgccgg agccgtggct tcgatatgca gcgctggacg tcgagttgct gatcgagttg
     2221 cgcgacgctt tggaatccgc actcgaccgt caaggcaagc tcgcctgggc tcacgaggaa
     2281 ttcgagcacg tacgcactgc accgcccgcg gccatttcgg tcgagcgttg gcgccggctc
     2341 tctggcatcc acaagatccg gcaccgccgt caactcgcga tcgtacggtc gatgtgggtc
     2401 accagagatc ggattgcccg cgagaccgac acctcaccga gccgattgct acctgatgca
     2461 tcgatcctcc aggtagcact cgagacgccc gccaatgccc gcgagctcgc cggctccgcc
     2521 gacttccacg gccgtggcag ccggcgctac ctagcgcaat ggtgggccgc gattgccgac
     2581 gccctggccg aggacgtcga cgggttgccc gagatgaccc ggccgagcga aggtccgcca
     2641 cccgggcacc ggtgggctga gcgcgaccgt gccgcggccg atcgactcgc ggcggccaag
     2701 gctgtcgtcg ccgcactcgc cgacgagcat ggactccctg ccgagaatct cctccagccc
     2761 gacgccgtgc gccgactggc ctggcgaccg ccgtcggatc tgttcgccgg cccggtcggt
     2821 gatgacttgc gcgggtttgg cgcgcgagag tggcagatcg ccctcacggc gggcccgctc
     2881 gcccacgccc tgctgcgggc cgcgacgcac gaaggcacac acgaatcggc ccacgaagcc
     2941 ggcgacccca gcatcgaggc cagtaacgaa ccggggcaga ccgtagcaac cgactgacaa
     3001 caggcgactc cacccgcaat ttcacccctt gcgcgctagt gcgatttgac cacccacagg
     3061 taccgtcacc tccatgtcga tgcccgtggg gggcccgaac cgtgaggaac gcacggctct
     3121 tcgccgctgc atgctcgcag tctcggtgct cgatgacgtc gacatgctcc cgaccgacga
     3181 cgggatggtg ttgagcggcg ttccccagct gacggtgaca ttcgctgaca tcgccgctgc
     3241 catcggcgct actgatcccg ccgggccgac tgcgcgccac cgccttgagt tgtggttgcg
     3301 cgcgcgacgg gcaatcgccg acaagtctct tgacgagctg gccgagacca tcaggccggt
     3361 aggactgccg gtcggtcacg acgtgcatcc gggcccaagc tgggtacggc agccgattct
     3421 cggtggctgc ctcgacctgg gcgtcggatt cgtcgggctg ttccgagacg tacccgacgc
     3481 agtcgtcgtg acgccgcaaa gcgtgctcga cgccgccggg atcgacccag gcccgtggtg
     3541 gcgcggcgcg accgagtacc tcgagaacat gggggcgctg gcaaccaccc gttggcgccg
     3601 cgacccccgc agccccgtgc gcccgatggg cgactgcgac gtgatcacgc tgctcgcgtc
     3661 gtcactgttc cgcggcgcgt tgtgtgccga cgcgggcggg atgcgcgcga tcgcggctcc
     3721 cgtgcgcacc cgagggtggc tcgatctgtc caggatcgat ccggcgttcg cccagaccgc
     3781 ggccgccctg gccgacgacg agagccgcgg gttcccacgg ccgctgctac tcacagttga
     3841 cgaagtctcg atggtctccg agggcggccg cccggccgag atcgtgctgc gtgatccagc
     3901 aaccgacgaa aagcgctggc tacgcgacgt cctctatcac tgaccgcgcc aaccacccca
     3961 cccgcgcgaa cccggccgcg ccggtcgccg agtgttcgca atcggttgag aaaacgcccg
     4021 tagcagagcc gattgtgcac actcggcgag gtcgaccgcg cgcggcgggc ggtggaccag
     4081 gtttagtcgg tgacgggttg cgtcaggtcg agcgcgtcga tcccggcgac cgactcgacg
     4141 acctgacgcg cgatctcctg agccgtgagc ccgatctcgc tcaggatctc ggcgcgctta
     4201 ccctgcgcca agaaccgttg cgggatcgat tgcacctgga ccggtgtcga gacacccgcg
     4261 tcgcgcagcg ccatcgacac cgtcgaacct gcgccaccct gccggccatg gtcctcgatc
     4321 gaaacgacaa gtgagtggga cgccgcgagg ccggcgatcg ccggattaac cggcagcacc
     4381 caccttgggt caacgaccgt cgaggcaatg ccctgatcgg ctagccggcc cgacacctca
     4441 agagcgagcg cggccatcgg cccgaacgcc acgattagca cgtcagcggc ctcatcgcgg
     4501 cgcaagacgt cgagaccgcc gatgcggtcg atccgctcga tgtcgcgggg cagggcgccc
     4561 ttcgggaaac ggatc
//
LOCUS       DASWVF010000540         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_498735, whole genome shotgun sequence.
ACCESSION   DASWVF010000540 DASWVF010000000
VERSION     DASWVF010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498735"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VGD55_09560"
     CDS             complement(<1..565)
                     /locus_tag="VGD55_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870631.1"
                     /translation="MRWGIALTAWLVASAATPAAAAVTSTWNGGDGNWSNAANWSAGI
                     PGPTDTASFSNSTLPCNIGSSGAIAVSVAAIAISKGVTINLNSGSSLTVSGTWTQSAG
                     IFNGGTGAIQITGDLALSGGTFNSTSGLLKIAGAFNKMGGTFSGGTGRVMLASTTSQA
                     LTITGTTTFNNLYINDGLIGYWKLDDSG"
     gene            complement(615..1826)
                     /locus_tag="VGD55_09565"
     CDS             complement(615..1826)
                     /locus_tag="VGD55_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870632.1"
                     /translation="MIDHMKVTLLRPDGKHHDEMNFKLKPAAGYFTAPMGHLVHGQAM
                     NVDAIIKGVDKGDDHLSVDDTVQYRPDLAVSRIDVPSAPTIGLPTSIAATVREMMGDL
                     GATADCLLSIDGTVVDRVPSIWVDAAGVVTCHFTATFKTAGQHTLRVDVGNVRPGDYD
                     MSNNGASATVTVGAGFAFTASASDATYSGADVTQVLDGSGNVLYDDNETFSGSVQSAS
                     LNGSWGMPVKFPLASVSVTAQSGSDSWSLIGLPSLPAGSTDPSNGTCGSGSDTTGFNW
                     VTVCTANVNGAPATTVAVSEFAGEVTYHSDGVCQQTTALADCAGGFSWNTGGSDVYAA
                     RHPFAGNLTLTVNVTDAAGANLQAFPVLPMTPFVSQYDSPQSCDPLGDGTQNCSKHTY
                     TETGVKAAVTP"
     regulatory      complement(2222..2297)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          405 a          806 c          930 g          515 t
ORIGIN      
        1 ttcccgaatc gtcgagcttc cagtaaccga tcaagccgtc gttgatgtag aggttgttga
       61 acgtcgtcgt acccgtgatc gtcagggcct ggctggtcgt ggacgccaac atcacacggc
      121 cagtgccgcc gctgaacgtg ccccccatct tgttgaaggc gccggcgatc ttcaagagac
      181 cggaggtgga gttgaatgtc cctcccgaca gggccaggtc gccggtaatc tggatcgcgc
      241 cggtgccccc gttgaagatg ccggcgctct gcgtccacgt accgctgacg gtgaggctgg
      301 aaccggaatt gaggttgatc gtgacgccct tcgaaatcgc gatggcggcg accgacacgg
      361 cgatcgcccc ggacgagccg atattgcagg ggagcgtgga gttgctgaag cttgccgtgt
      421 cggtggggcc gggaatgccc gccgaccagt tcgcggcgtt gctccagttt ccgtctccgc
      481 cgttccaggt gctggtgacc gccgcggcgg ccggcgtggc cgcggaggcg accagccatg
      541 cggtgagggc gatgccccac cgcatggcca ggactgcttt ggtcgcgctc cgcttcatcg
      601 aatccgacga gggctcaagg ggtgacggcg gccttgacgc cagtttcggt ataggtgtgc
      661 ttcgagcagt tctgggtgcc gtcccccaag ggatcgcagc tctggggcga atcatactga
      721 gacacgaatg gcgtcatcgg caggaccggg aacgcctgca ggttcgctcc cgccgcatcc
      781 gtgacgttga cggtcaaggt gaggttcccg gcgaaggggt ggcgggcggc gtacacgtcg
      841 ctaccaccgg tgttccagct gaaaccaccg gcgcagtctg cgagggcggt tgtctgctgg
      901 cagacgccgt cggagtggta ggtcacctcg ccggcgaact cggacaccgc aacggtggtc
      961 gccggagcgc cgttcacgtt cgccgtgcac acggtaaccc agttgaaccc cgtggtgtca
     1021 ctgccggagc cgcaagtccc gttgctgggg tcggttgagc cggccggcag gctgggaaga
     1081 ccaatcagag accagctgtc gctgcccgat tgagccgtca ccgacacgct cgcgagcggg
     1141 aacttgacgg gcatccccca gcttccgttc agggatgcgc tttgcaccga gccgctgaac
     1201 gtttcgttgt cgtcatagag aacgttgccg ctgccgtcga gcacctgcgt cacgtcggcg
     1261 cccgagtagg tggcgtcgga ggcgctcgcc gtgaacgcga acccggcacc gaccgtcacg
     1321 gtcgcgctgg cgccattgtt gctcatgtcg taatcgccgg ggcgcacgtt gccgacgtcg
     1381 acgcgcagcg tgtgctgtcc ggctgtcttg aacgtcgcgg tgaagtggca ggtgaccacg
     1441 ccggcggcgt ccacccagat ggagggaacg cggtcgacga ccgtgccgtc gatggacagc
     1501 aggcagtcgg cggtggcgcc caggtcgccc atcatctcgc gcacggtggc agcgatgctg
     1561 gtgggcaatc cgatcgtggg cgccgacggc acgtcgatgc ggctgaccgc caggtccggc
     1621 cggtattgca ccgtgtcgtc caccgacagg tggtcatcgc ccttgtcgac gcctttgatg
     1681 atggcgtcga cgttcatggc ttgaccgtgg accaaatggc ccatcggcgc cgtgaagtat
     1741 cctgccgccg gcttgagctt gaagttcatc tcgtcgtggt gcttcccgtc gggtcgcaag
     1801 agtgtgacct tcatgtgatc gatcaccccg ttgggcgccg aaccgtcgtc gaaagtgccg
     1861 gtcgttgcct cgaagaccgc gttcttctgc acgtcgagca gcgttcgaac ggcgagagtt
     1921 gcgttgccgg cgcgagaccg atgaaacatg tggcggtgtc gatagtgttc acagcgtttg
     1981 tggaagtggt ggtctctgtc gtggtcgggg tcgtgcggca tgccgcacgc ggcgtcgggg
     2041 atgtctttga acccgccagg tgggtccgcg tgtgccgccc gggcgccgcc gaggagagtc
     2101 gccgtggtcg acgccaaata cagcgcgagc caccggccgc ctccgtgtcg ggggctcttg
     2161 tgttcagggg tgtgggctgt ctgtcctttg ctcgacctcg tgtccatggt tgtccctctc
     2221 ttgtggtaac ccggccgact cagtgagtcc cgttggcttt gcgtcccccg atcgctcggg
     2281 gtttgccttt gtcacaagca cttccctacg aagtactgtg ctgtctggaa cagaatcgat
     2341 gccgacgcac gcgccagatg ggaccggcat ccacgccgtg gaggcggggc ggtcactctg
     2401 tctgggccgt tgtcgtcgga cggctggcct tgtggaggcg cgcacgggga tctcacggcg
     2461 ccccccgacg cctattggtt ccatcggagg gctgccgttc ggtcttgaga ggatcgacag
     2521 aagaaatttg cgttagctgc gcaggtagcg cgtgagcagc tcgacggcca gcttcttgtg
     2581 gtcgctctgc cagcccgccg aacacaggtc ggtgcggaac aggaaggaga gggtgtgggc
     2641 gttggagcgg tggagg
//
LOCUS       DASWVF010000541         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_319926, whole genome shotgun sequence.
ACCESSION   DASWVF010000541 DASWVF010000000
VERSION     DASWVF010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319926"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGD55_09570"
     CDS             <1..533
                     /locus_tag="VGD55_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY0870633.1"
                     /translation="IRESIARVVPGFDDFNARVRVPGGFTLPHPARDSRTFKTESGRA
                     EFTVNGLAVLRPGPGCLILQTIRSHDQYNTTIYGLNDRYRGIHHGRRVVFVHPDDIAT
                     LGLADGDIVDLVSVWTDGVARRAPVFRVVSYPTAQGCAAAYFPETNVLVPLDSTADTS
                     NTPTSKSIIVRLEPTP"
     gene            577..1050
                     /locus_tag="VGD55_09575"
     CDS             577..1050
                     /locus_tag="VGD55_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0870634.1"
                     /translation="MSSSRGLATRQADCDAARQADSGFSLIELLVVMIIIGILAAIAI
                     PIYLSQQQKAADAQAKSDVHSAAIAEESYFADFQTYIPEPLSTTPPTALAAQGFKMSS
                     KTLDVTVYTYASGSTTGGSTTLSQNGGYCVKVTSGSTKAFYYTSFLGGITNTACL"
     gene            1108..1767
                     /gene="nucS"
                     /locus_tag="VGD55_09580"
     CDS             1108..1767
                     /gene="nucS"
                     /locus_tag="VGD55_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY0870635.1"
                     /translation="MRLVIARCSVDYVGRLTAHLPSAQRLILVKADGSVSIHADDRAY
                     KPLNWMSPPCTLQEAPGVWTVTNKAGEQLVLTIEEMLHDSSHELGVDPGLVKDGVEAH
                     LQVLLAAHPNALGEGMTLVRREYPTPIGPVDLLCRDVSGVSVAVEIKRRGDIDGVEQL
                     TRYLELMNRDALIAPVRGIFAAQEIKPQARVLAVDRGIECVTIDYDALRGADDPTARL
                     F"
     gene            complement(1764..>2267)
                     /locus_tag="VGD55_09585"
     CDS             complement(1764..>2267)
                     /locus_tag="VGD55_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870636.1"
                     /translation="AVLQTTTPSLRQRVGSSLARLWHRVPGGRFGALAVLTLAVVIGT
                     GVSTVGRRWLRRRRRYAGIDRGRRDDGPILAAYIRLDIALRGVERARAPSESLGEFAG
                     RLGGFVATPGEVAAAIRCLERETYGVDPPSPAESGAAIAVFDRQSQRAGSELVAVAVG
                     SGTTNPR"
BASE COUNT          430 a          774 c          687 g          376 t
ORIGIN      
        1 ggattcgcga gtcgatcgcg cgagtggtgc cgggcttcga tgatttcaac gcgcgagttc
       61 gggtaccggg cgggttcacg cttccgcatc cggcccgcga ttcgcgcacg ttcaaaacgg
      121 agagcggtcg ggccgagttc accgtcaacg ggttagcagt tctgcgaccc ggtcccggct
      181 gcctcatctt gcagaccatt cgttcgcacg accagtacaa caccacgatc tacggactta
      241 atgatcgcta ccgcggcatt caccacggcc ggcgcgtcgt gttcgtgcac cctgacgaca
      301 tcgcgacgct cggtctcgca gatggggaca tcgtcgacct ggtcagcgtg tggaccgatg
      361 gcgtcgcgcg ccgtgcacct gtgtttcgcg tcgtgtccta ccccaccgcg cagggctgtg
      421 cagccgcgta cttccccgaa accaacgtcc tggtgccgct cgacagcaca gcggacacca
      481 gcaatacgcc gacgtccaaa tcgataatcg tgcgcctgga gccgacacct taaagcaagc
      541 ctaaagtgcg gcattcggaa atccgaagat gtacgcatgt cttcctctcg cgggctcgcc
      601 acgcgtcaag cggactgcga cgctgcccgg caggccgact cgggcttcag cctgatcgag
      661 cttctagtcg tcatgatcat catcggaatc cttgcggcca tcgcgatccc gatctacctg
      721 agccagcagc aaaaagcggc ggatgcccag gcgaaaagtg acgtgcacag cgccgcgatc
      781 gccgaagaga gctacttcgc cgacttccag acttacattc cggagccgct ctcgacgacg
      841 ccaccgacgg cgttggccgc tcagggcttc aagatgtcga gcaagaccct cgacgtcacg
      901 gtctacacgt acgcctctgg gtcgacgaca ggcggatcga caaccctctc gcaaaacggt
      961 gggtactgcg tgaaagtaac gtcgggctca accaaggcct tctattacac gagcttcttg
     1021 ggcggcatca ccaacaccgc ctgcctatag cgacgttcgc cagccccacg tcgtccggtc
     1081 aatgttcttg gcgctaacgt tcgcggtgtg cggctggtga ttgcgcgatg cagtgtcgac
     1141 tacgtcggcc ggctgacagc ccatttgccc agcgcgcagc gactgatcct cgtcaaggcc
     1201 gacggctcgg tctcgatcca cgccgacgat agggcgtaca agccgctgaa ctggatgagc
     1261 ccgccgtgca cactgcagga agctcccggc gtgtggaccg tgacgaacaa ggccggcgaa
     1321 caactcgtgc tcaccatcga agagatgctg cacgactcca gccatgaact cggcgtcgat
     1381 cccggtctgg tcaaggacgg cgtagaggca catctgcagg tactgctcgc ggcacacccg
     1441 aacgccctcg gcgaaggcat gacgttggtt cgccgtgaat acccgacacc gattggcccg
     1501 gtcgatctgc tgtgtcgcga cgtatccggc gtcagcgtgg cggtcgagat caagcgtcgc
     1561 ggcgacattg acggcgtcga gcagctgact cgctacctcg agctgatgaa ccgcgacgca
     1621 cttatcgcac cggttcgtgg catcttcgcg gcccaggaga tcaagccaca ggctcgagtg
     1681 ctggccgtcg accgcggcat cgagtgcgtc accatcgact acgacgcgtt gcgcggcgcc
     1741 gacgatccga ccgcccgcct cttctagcga gggttggtcg taccgctgcc cactgcgacc
     1801 gcgacgagct cagagcctgc acgctggctc tgccgatcga acaccgcgat tgccgcgccc
     1861 gactccgcag gagatggcgg gtcgacgccg taggtctcac gctcgaggca cctgatcgcg
     1921 gccgcgacct cacccggagt cgcgacaaac ccgcccaggc gacctgcgaa ctcgcccaaa
     1981 ctctcactcg gcgcgcgggc ccgctcgact ccgcgcaacg caatgtccag ccgaatgtag
     2041 gcggccagga tcggaccatc gtcacgacga ccgcgatcga tacccgcgta acggcgccgg
     2101 cgccgcagcc accgacgtcc gaccgtgctg acgccggtgc cgatgacgac cgcgagcgta
     2161 agaacggcga gagccccgaa gcgtccgccc gggacgcgat gccatagtcg ggccagcgat
     2221 gaaccgacgc gctgccgtag agacggcgtc gtcgtctgca agaccgc
//
LOCUS       DASWVF010000542         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_371247, whole genome shotgun sequence.
ACCESSION   DASWVF010000542 DASWVF010000000
VERSION     DASWVF010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371247"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGD55_09590"
     CDS             <1..473
                     /locus_tag="VGD55_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY0870637.1"
                     /translation="LCDPRAMRSTLFEFAGGEPAFLALATAHHARCLADPELNHPFSH
                     PGNPQHLERLASYWGEVLGGPPTYSQSCGDQTFLLRMHAGNGDMSDLGRRFVECFVLA
                     ADDAQLPDDPAFRAALRAYMEFAVGEMISYPDSDDVIPTDAPTPRWSWDGLQAS"
     gene            complement(470..1276)
                     /locus_tag="VGD55_09595"
     CDS             complement(470..1276)
                     /locus_tag="VGD55_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0870638.1"
                     /translation="MQDREGSAGERFVRLELSAPGVGTIRLDRPPVNAMNVATQDQLH
                     EVAVEVAARDDIRAVVIYGGPRSFAAGVDVKEMAAMTHADMLARVGPMQAAFTAVADI
                     AKPVIAAITGYALGGGCELALAADIRFCADDAKLGQPEMLLGVIPGGGGTQRLARLIG
                     PARAKELIFTGRTVDADEAARLGLIDRILPSAQVYEAAVAWAAQFADGPALALAAAKR
                     TIDEAMASDLAAGLAAERAAFADMFATHDRTIGMQSFITSGPGNAHFTGH"
     gene            1345..1971
                     /locus_tag="VGD55_09600"
     CDS             1345..1971
                     /locus_tag="VGD55_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY0870639.1"
                     /translation="MALRGRLLAAAGVIGPCVFIADWAILGAHAKNYSPVSDAISELA
                     RMHATTRPAMTAGFLVFGAALPTYAVALRQALPGQAWKFAAANGVATLGVAAFSLGTP
                     TSGDIHGVFAGLAYASLAATPIAASVVLRRRGQYKLARTSIATGIAVGTALVVSVVGP
                     THVHGLFQRIGLTIGDAWLIASALSMMRTLPPTPPQPALSGQQVDPAG"
     gene            1976..>2596
                     /locus_tag="VGD55_09605"
     CDS             1976..>2596
                     /locus_tag="VGD55_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY0870640.1"
                     /translation="MDIWPGRPFPLGPTASADRAGTNFALFSQRASAVDLCLFDASGN
                     ETQVPLRASTFHVWHGYVPGVAPGTHYGYRVDGDFNPAGGLRFNHNKLLVDPYARAID
                     GAFTLDDAVFGYPVATHDDTARDDRDSAPFVPRSVVVRDDFDWGADRPPAVAWADTLI
                     YEVHVRGFTMAHPDIPQQLRGTYAGLVHPAALEHLTKLGVTTLELMP"
BASE COUNT          405 a          945 c          816 g          430 t
ORIGIN      
        1 ccctgtgcga ccctcgcgct atgcgttcta cgctcttcga gttcgccggt ggtgaaccgg
       61 cctttctggc gttggccacc gcacaccacg cccggtgcct cgccgacccc gagctgaacc
      121 atcccttctc acatccggga aatccccagc atctcgagcg gctggcctcg tactggggag
      181 aggtcttggg cgggccgccg acgtactcgc aatcgtgcgg ggaccaaaca ttcctcctgc
      241 gcatgcacgc cggcaacggt gacatgagtg atcttggacg ccgatttgtc gagtgcttcg
      301 tgctcgcagc cgacgacgcc cagctgcccg acgaccccgc ttttcgcgcc gcacttcgcg
      361 cgtacatgga attcgcggtc ggcgagatga tctcctaccc cgacagcgac gacgtcatcc
      421 caaccgacgc accgacgccg agatggtcgt gggacgggct gcaagcgtcc tagtgcccgg
      481 tgaagtgggc gttgcccggg cctgatgtga tgaacgactg catcccaatt gttcgatcat
      541 gcgtggcaaa catgtcagca aaggcggcgc gctcggctgc aagcccggcg gccaggtcgg
      601 acgccatcgc ttcgtcgatc gtgcgcttcg cggccgcaag cgcgagagct ggcccgtcgg
      661 cgaattgagc ggcccacgcg accgctgcct cgtaaacctg cgcgctcggc aagatccggt
      721 cgatgagtcc gagtctggcc gcttcgtcgg cgtccaccgt gcggccggtg aaaatgagct
      781 ccttcgcgcg cgccggcccg atcagtcgcg ccagccgctg ggtgccgcct cccccaggga
      841 tgacgccgag cagcatttcg ggttgcccga gcttcgcgtc gtccgcgcag aatcgaatat
      901 cggcggcgag cgcgagctca caaccgccgc cgagcgcgta cccggtgatg gccgcaatca
      961 ccggcttcgc aatgtcagcc accgcggtga acgcggcctg catcggtccg acgcgggcga
     1021 gcatgtcggc gtgagtcatc gccgccatct ccttgacatc gacaccggcg gcgaacgacc
     1081 gcggacctcc gtagatcacg acagcccgga tgtcgtcgcg cgccgcaacc tcgactgcga
     1141 cctcgtgaag ctggtcttgc gtcgcgacat tcatcgcgtt caccggcggt cggtcgagcc
     1201 ggatcgtgcc aacccccggc gcgctcagct cgaggcggac gaaccgctcg ccggctgacc
     1261 cttcgcgatc ctgcatcgcg ccaggctatc cgcgaaacgg agcggcagcc ggttggcgtc
     1321 ccgcatcaag ggcacaatgc tggcgtggcc ctacgcggac gtctcctcgc cgcggccggc
     1381 gtgatcgggc cctgtgtctt tatcgccgac tgggcaatcc tgggcgcgca cgcaaagaac
     1441 tactcccctg tcagcgacgc gatcagcgag ctggcccgca tgcatgccac gacccgcccc
     1501 gccatgactg ccggattcct cgtgttcggt gcggcgctgc ccacctacgc ggtcgcgctt
     1561 cgccaggcgc tacccggtca ggcctggaag ttcgccgcgg ccaacggcgt cgcaacactc
     1621 ggcgtggcgg cgttctccct cggcacaccg acgtcgggcg acatccacgg agtcttcgcc
     1681 ggcctcgcct acgcctctct agcggcaacg ccgatcgccg cgagcgtcgt gctccgtcgg
     1741 cgcgggcagt acaaacttgc gcggacgtcg atcgcaaccg gcatcgctgt cggtaccgca
     1801 ctcgtggtca gcgttgtggg cccgacccac gtgcacgggt tgttccagcg catcggactc
     1861 acgatcgggg acgcgtggct catcgcgtca gccctgtcga tgatgcgaac gctgccaccg
     1921 accccacccc agcccgccct gtcaggtcag caggtcgacc cggcaggata agccgatgga
     1981 catctggcca ggccgcccgt tcccgctcgg cccgaccgcc tccgctgacc gagccggcac
     2041 caacttcgca ctgttctcgc agcgagccag tgccgtcgac ctgtgcctct tcgacgcaag
     2101 cgggaacgag acgcaggttc cgctgcgcgc atcgacgttt cacgtgtggc acggatacgt
     2161 cccgggcgtg gcgcccggaa cgcactacgg ctatcgcgtc gacggcgact tcaacccagc
     2221 aggcggcttg cgcttcaatc acaacaagtt gctcgtcgat ccctacgccc gtgcgatcga
     2281 tggtgcgttc accctcgacg acgcggtgtt cgggtacccg gtcgccacgc acgacgacac
     2341 ggcgcgcgac gaccgggact ccgcgccgtt cgtgccgagg tcggttgtcg tgcgcgacga
     2401 tttcgactgg ggagccgacc gaccgcctgc agtggcgtgg gccgacacgc tcatctacga
     2461 ggttcacgtg cgcggattca cgatggcgca ccccgacatt ccgcagcagc tgcgcggcac
     2521 ctacgcgggt ctggtccatc cggccgccct cgagcacctg accaagctcg gggtcacgac
     2581 gctcgaactc atgccc
//
LOCUS       DASWVF010000543         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_91019, whole genome shotgun sequence.
ACCESSION   DASWVF010000543 DASWVF010000000
VERSION     DASWVF010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91019"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGD55_09610"
     CDS             <1..275
                     /locus_tag="VGD55_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0870641.1"
                     /translation="SAHTRERIILVGDVPSPIDPPKGCRFHPRCPKAAQQCVDFEPEL
                     VARGDDPPTHETACHFPVADDEDLALARPTIAAAERVVAEPGILST"
     gene            310..1293
                     /locus_tag="VGD55_09615"
     CDS             310..1293
                     /locus_tag="VGD55_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870642.1"
                     /translation="MTQGIGDAISAGESDQLPEVLVPGLEGTKAIRGRSPFALAFDRL
                     RRDRGAMISLGVIILIILVAIGAPLFGVLTGHGPNHQYRTPNALSASGQPHAPSGTFW
                     FGTDDQGRDVLVRTAYGARISLIVGIGATAITIVVGVLVGLVTGYFGGIVDTVLARII
                     DMVLSIPYLLFAISLFAILGHLNIWLVMLAIAAFGWASVGRIIRGQVISMREREYIEA
                     ARSLGASDWRIIFVDILPNVIAPAIVFSTLLLPVSVLSEAALSYLGIGIPPPTADWGQ
                     MISDAQPLYQVAWWYLFFPSLALLITTLAFNIFGDGVRDAFDPRSDRLFAK"
     gene            1303..2265
                     /locus_tag="VGD55_09620"
     CDS             1303..2265
                     /locus_tag="VGD55_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870643.1"
                     /translation="MIRFLIRRLSLGVVVMFMVTVAIYGMFYLGSPMDIARRLAGRNA
                     TPDLVQQIYKNLGLNKPLWMQYRHFVWRLLHGDLGRDYYHQLPVTTVLKQDAPITISL
                     AIGAAIIWFLLGVSTGVYSSIRPRSFMDRSFTASALFFYSLPVFVLGVTLRYFLFYRL
                     TLHHFKWFPAEGYVSLTSSPFEWARHLILPWLTLALISAAAYTRLSRTSMLEVLGEDY
                     IRTARSKGLTERRVVLRHALRSALTPVVTQFGIDFASVLGGAILTESVWGLPGLGRES
                     VQAIENQDLPVIIGIVIVAAAAIILANILVDMAYAVLDPRVRLH"
     gene            2360..2587
                     /gene="secG"
                     /locus_tag="VGD55_09625"
     CDS             2360..2587
                     /gene="secG"
                     /locus_tag="VGD55_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0870644.1"
                     /translation="MIGALEIIISVTSLLLVVLILLHRGKGGGLSDMFGGGFTSSFAG
                     SSVVERNLDRFTLITAFVWFASIVGLLLLFK"
BASE COUNT          414 a          849 c          791 g          590 t
ORIGIN      
        1 ggtccgcgca cacccgcgaa cggatcatcc tggtgggcga cgtgccgtcg cccatcgatc
       61 caccgaaggg ttgccgcttc cacccgcgtt gtcctaaagc ggctcagcaa tgtgtcgatt
      121 tcgagcccga actcgttgca cggggcgacg acccgccgac ccacgagacg gcatgccatt
      181 tccccgtcgc cgacgacgag gatctcgcct tggccagacc aacgattgcg gctgccgaac
      241 gggttgtcgc cgagcccgga atcttgtcga cgtgaaccga atcatcacaa ctgcgcagga
      301 ggtgccgcga tgacccaggg tatcggcgat gccatctccg ccggcgaatc cgaccaactt
      361 cccgaggttc tcgttccggg acttgaggga accaaggcga tccggggtcg cagcccgttc
      421 gcgctcgcgt tcgaccggtt gcgccgcgac cgcggtgcga tgatctcgct cggtgtgatc
      481 atcttgatca ttttggtcgc gatcggggcg ccgctgttcg gcgtgctcac tggccacggg
      541 cccaatcacc aataccgcac gccgaatgcg ctgtcggcat ctggacaacc gcatgcaccc
      601 agcggcacct tctggttcgg caccgacgac caggggcgcg acgtgctggt gcgcactgcg
      661 tacggtgcgc ggatttcgtt gatcgtcggt atcggggcta cggcgatcac catcgtcgtc
      721 ggtgtgctcg ttggtctggt gacgggctac ttcggcggca tcgtcgacac cgtgctggct
      781 cgcatcatcg acatggtgct gtcgatcccg taccttctct tcgcgatctc gttgttcgcc
      841 atcttgggtc atctcaacat ctggctcgtg atgctcgcca ttgccgcgtt cggatgggcg
      901 tcggttggcc gcatcattcg cgggcaggtg atctcgatgc gagaacgcga gtacatcgag
      961 gctgcccgat cgttgggcgc gagtgactgg cggatcatct tcgtcgacat cctgccgaac
     1021 gtgatcgctc cggctatcgt gttctcgacg ctgctgttgc cagtcagcgt gctgagtgag
     1081 gctgcgctct cgtatctcgg aatcggcatc ccgccgccga cggccgactg gggccagatg
     1141 atctctgatg cgcaaccgct ctatcaggtc gcctggtggt atctcttctt tcccagtctg
     1201 gcgctgctca tcacgacgct ggcgtttaac atcttcggcg acggtgtgcg cgatgcattc
     1261 gaccctcgca gcgaccgtct gttcgcgaag tagggcctgc cgatgatccg ctttctcatt
     1321 cgccgcctga gcctgggtgt cgtcgtcatg tttatggtga cggtcgcgat ctatggaatg
     1381 ttctacctgg gctcaccgat ggacatcgcg cgacggcttg ccggccgcaa cgcgaccccc
     1441 gacttggttc agcagatcta caagaacctc ggtctgaaca agccgctgtg gatgcaatac
     1501 cgccacttcg tttggcggct tttgcatggc gatctcggaa gggactacta ccaccaactg
     1561 ccagtcacga cggttctgaa acaggacgcc ccgatcacca tctcgctcgc gattggtgcg
     1621 gcgatcatct ggttcttgct cggcgtctcg accggcgttt actcgagcat ccggccgcgc
     1681 tcgttcatgg accgatcatt tactgcgagc gcgctcttct tctactcgct cccggtcttc
     1741 gtactcggcg tcaccttgcg gtatttcctg ttctaccgac tgactctgca ccacttcaag
     1801 tggttccccg ccgagggata cgtctcgtta acctccagcc catttgagtg ggcccgacat
     1861 ctgatccttc cgtggctcac attggccctg atctctgccg ctgcctatac aaggctgagc
     1921 cgcacgtcga tgcttgaggt tctcggcgag gattacattc gcaccgcgcg ttcgaagggc
     1981 ttgaccgagc ggcgggtcgt ccttagacac gcgcttcgca gtgccctcac acccgtggtc
     2041 acgcagttcg gcatcgactt tgcaagtgtg ctcggcggcg cgattctgac cgaaagcgtg
     2101 tggggcctgc cagggcttgg ccgcgagtcg gtgcaagcca tcgagaacca agacctgccg
     2161 gtgatcatcg gaatcgtcat cgttgccgct gccgcgatca tcctcgcgaa catcctggtc
     2221 gacatggcct acgccgtgct cgaccctcgg gtccggctgc actgaggggg tatccttata
     2281 aatagcgcgc cgccttgtgt ggcgccgccc agaaacaccc gcgatctacc cgagtacaca
     2341 cccggccaag gagacgctcg tgatcggcgc gctggagatc atcatctcgg tgacgagctt
     2401 gttgctcgtc gtcctgatct tgctgcaccg cggcaagggt ggtggcctgt ctgacatgtt
     2461 cggcggtggc ttcacgtcga gcttcgcggg ctcctcggtc gtcgagcgca acctcgaccg
     2521 gttcaccctc atcacggcct tcgtgtggtt cgcctccatc gtcgggctgc tgttgctctt
     2581 caagtaggag agggtttgtc gcgaggccaa tcggctcgca cgcttttgtc gacacggagg
     2641 agat
//
LOCUS       DASWVF010000544         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_294849, whole genome shotgun sequence.
ACCESSION   DASWVF010000544 DASWVF010000000
VERSION     DASWVF010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294849"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1397
                     /gene="obgE"
                     /locus_tag="VGD55_09630"
     CDS             33..1397
                     /gene="obgE"
                     /locus_tag="VGD55_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0870645.1"
                     /translation="MPTFVDRVVLHASAGNGGNGCASIHREKYKPLGGPDGGTGGRGG
                     NVALVVDPDTTTLLALHHAPHRRATSGKPGQGSNRDGANGEDMILPVPDGTVVKSQDG
                     VVLADLVGAGTTYVVARGGRGGLGNAALASPRRKAPGFALLGEPGAVEDVVLELKTVA
                     DVALVGYPNAGKSSLVAALSAARPKIADYPFTTLVPNLGVVQAGESRFTVADVPGLVR
                     GASEGRGLGLEFLRHVERCTALVHVIDCATMEPDRDPVSDLEAIEAELAAYDETDLGG
                     VPLHERPRIVALNKVDVPDARDLAEMVRAEIEARGIKVLIVSAATREGLRELSFAMAE
                     LVEQARAARPVDESTRIVLRPRAVDDSGFTVTREGDAFVVRGERPERWIKQTDFTNDE
                     AVGYLADRLNRLGVEAELAKAGAEEGAEVVIGDVAFDWEPGMLSGAEARIGPRGTDAR
                     LDGR"
     gene            1403..2530
                     /gene="proB"
                     /locus_tag="VGD55_09635"
     CDS             1403..2530
                     /gene="proB"
                     /locus_tag="VGD55_09635"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0870646.1"
                     /translation="MSERRAAVTAARRIVVKIGSSSLTRPEGGLDQANIDALASALVG
                     RLGDGTACVLVSSGAIAAGLRPLGLTTRPRDLATQQAAASVGQGLLVESYARAFGRHD
                     VRVGQVLLTSDDMIRRVHYLNAERTLTKLLELGVVPVVNENDTVVTEEIRLGDNDRLA
                     ALVAHLVRADLLVLLSDVDALYTAPPSRAGSHRLDDVHGEADLVDVDLGGVGRVGIGT
                     GGMITKVEAARLATSAGIPVVLASAGEVVAALAGEHTGTLFHPTGVRVASRLLWLAHA
                     TTPRGRLVLDPGAVAAVVDRRLSLLPAGITAVEGEFAAGDPVDLVDNAGQAVARGLVN
                     YDATELPELLGRSTRELAAKLGPAYEREVVHRDDLVLLTNR"
     gene            2704..3057
                     /locus_tag="VGD55_09640"
     CDS             2704..3057
                     /locus_tag="VGD55_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870647.1"
                     /translation="MSGLQAKLWHVTVTVGGESMETSLVQAALERLSGEQPFLLSGRY
                     GSDRAEVRYWEEAPECADAAALALRLWGEHRASAGLPAWHVLGVEVVDREIFQRRGGE
                     GGVSLIAPAGGWRPF"
     gene            3111..4394
                     /locus_tag="VGD55_09645"
     CDS             3111..4394
                     /locus_tag="VGD55_09645"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY0870648.1"
                     /translation="MSTAASTPPSGDSAADVLATARRARVAANVLATTPRATKDSALQ
                     AVAAALIARAHEVLQANKVDVETAAAAGASESFIDRLRLDLRRIEAIAGDVVKTAGLP
                     DPVGEVVRGGVLANGLELRQLRVPLGVVGIIYEGRPNVTVDAAALCLKSGNAVLLRGS
                     SSALMSNQALVAILRDAISSVGLPADAVQLVPGAGHEPAKHLMRARGLVDVLIPRGGA
                     ELIRSVVEESTVPVIETGVGNCHIYVDADADLDMAVAILLNAKTSRPSTCNTAETVLV
                     HADVADAFLPRALAALRSAGVTVHGDAAVVSMDGAVVPATDADWDTEYLSLDIAAAVV
                     PSLDAAIAHIREHGTAHSEAIVTTSQAAARTFIAQVDAAAVLVNASTRFVDGAEFGFG
                     AEIGISTQKLHARGPMGLAELTSTKYVVVGDGHVR"
     gene            4405..5034
                     /gene="nadD"
                     /locus_tag="VGD55_09650"
     CDS             4405..5034
                     /gene="nadD"
                     /locus_tag="VGD55_09650"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0870649.1"
                     /translation="MNEAPPVLRPRIGVMGGTFDPIHHGHLVAASEVASKFGLDEVIF
                     VPTGAPWQKTDRQVSPAEDRYLMTVIATASNPRFSVSRVEIDRPGPTYTSDTLTQLRR
                     ELAESRGADPEIFFITGADALAQIMTWNNANMLVDLAHFVGVTRPGHPLADPRGLPEG
                     AVSLVEIPALAISSTECRERVGHNQPIWYLVPDGIVQYISKRGLYRSQA"
BASE COUNT          827 a         1691 c         1809 g          857 t
ORIGIN      
        1 ttgtttctag agatcctcga caggagggca cgatgccgac attcgtcgat cgtgtcgtcc
       61 tgcatgccag cgcaggcaac ggcggcaacg ggtgcgcgtc gatccaccgc gagaagtaca
      121 agccgctcgg cggacctgac gggggtaccg gtggccgcgg cggaaacgtc gcgctcgtgg
      181 tcgatcctga caccacgacg ctgctagccc tccatcacgc cccgcaccgg agggcgacgt
      241 ccggcaagcc gggccagggc agcaaccgcg acggtgccaa cggcgaggac atgatcctgc
      301 ccgtccccga cggcacggtg gtgaaatcac aggacggcgt ggtgctggca gacctcgtcg
      361 gcgccggcac gacctacgta gtggcgcgcg gaggccgggg cggcctcgga aacgcggcgc
      421 tagcctcacc tcgccgcaag gcgcccggct tcgccctgct cggcgaaccc ggcgcggtcg
      481 aagacgtcgt cctcgaactc aagaccgtcg ctgacgtcgc gctggtcggc taccccaacg
      541 ccggcaagtc gtcgctggtc gccgccctgt cagccgcccg ccccaagatc gccgactacc
      601 cgttcacgac gctggtcccg aatctcggcg tcgtgcaggc gggggagtcg aggttcaccg
      661 tggccgacgt tcctggcctg gtccgcggcg ccagcgaggg ccgcgggctc ggcctcgaat
      721 tcctgcgcca cgtcgagcgc tgcacggcgc tggtgcacgt aatcgactgc gccaccatgg
      781 agcccgatcg cgaccctgtc agcgatctcg aggcgatcga agcagaactc gctgcctacg
      841 acgagaccga tctcggcggc gtcccgctgc atgaaagacc gcgcatcgtg gcgttgaaca
      901 aggtcgacgt ccctgacgct cgcgatctcg ccgagatggt gcgagccgag atcgaggcgc
      961 gcggcatcaa ggtgctcatc gtgtcggcgg ccacccgcga gggtctgcgt gaactgtcgt
     1021 tcgcgatggc cgagctcgtc gagcaggcac gagccgcgcg gccggtcgac gagtcgaccc
     1081 gcatcgtgtt gcggccgcgc gcggtcgacg acagcggatt cacggtcacc cgcgagggcg
     1141 acgcgttcgt cgtgcgcggc gagcgacccg agcgctggat caagcagacc gacttcacca
     1201 acgacgaagc cgtcgggtat ctggccgacc gcctgaaccg gctcggggtc gaggcggagc
     1261 ttgcgaaagc cggcgccgaa gagggcgccg aggtcgtcat cggcgacgtc gcgttcgact
     1321 gggagcccgg catgttgtcg ggcgcagaag cccggatcgg accgcgcggc accgacgcgc
     1381 gcctcgacgg tcggtgacag gtatgtccga gcgtcgcgcg gcggtgacgg ctgcgcgccg
     1441 catcgtcgtc aagatcggat cgtcgtcgct caccaggcca gaaggtgggc tcgaccaggc
     1501 gaacattgat gcgctcgcgt ccgcgcttgt cgggcgtctt ggcgacggga cggcgtgtgt
     1561 gctggtgtcg tcgggtgcga ttgccgccgg gctccggccg ctcggactca cgactcggcc
     1621 gcgtgatctc gcgacccagc aggccgccgc gagcgtcggt caaggactgc tggtcgagtc
     1681 gtatgcgcgc gcgttcgggc ggcacgacgt gcgcgtgggg caagtgctgc tgacgtccga
     1741 cgacatgatc cgccgcgtgc actacctcaa cgcagaacgc acactgacca agctgctcga
     1801 gcttggcgtg gtccctgtcg tgaacgagaa cgacaccgtc gtgaccgaag agattcgctt
     1861 aggcgacaac gaccggctcg ctgctctcgt cgcccatctg gtgcgcgccg acctgctcgt
     1921 tctgctctcc gacgtcgacg ctttgtacac cgcgccgccc agccgtgcgg gatcgcaccg
     1981 gctcgacgac gtccatggcg aagcagatct tgtcgacgtc gatctgggtg gcgtcgggcg
     2041 agtagggatc ggtaccggcg gcatgattac caaggtcgaa gccgcgcggc tcgcaacgtc
     2101 ggccgggatt cccgtcgtcc tcgcatctgc tggtgaagtc gtcgcagcac ttgccggcga
     2161 acacaccgga acgctgtttc acccgacggg cgttcgggtt gcgagtcggc tgctgtggct
     2221 cgcgcatgcg acgacgccgc gaggtcggct cgtcctcgac ccaggcgcag tcgcggccgt
     2281 cgtcgacagg cggctttcgt tgctgccggc cggcatcacc gctgtcgagg gcgagttcgc
     2341 tgccggcgac ccggtcgatc tcgtcgacaa tgcggggcaa gcggtcgccc gtggcctggt
     2401 gaactacgac gccaccgagt tgcccgagct gctcggccgt tcgacgcgcg agctagcggc
     2461 gaagctcggc ccggcatatg aacgcgaggt cgtgcaccgc gacgacctgg tgctgctcac
     2521 gaaccgctga cgcccaagta caggcccgga accgcgacgg aaaagcgtag ttgcgaaggg
     2581 ggccagaggt accgagttca ggaaaaatgc ctccggaaat ggccccggac tgatcaacta
     2641 tgttccgccg ttgatggggg actcgggcga gggccgagtc gtgacgagta gggccaactg
     2701 gggatgagcg ggctgcaagc gaagctgtgg catgtcaccg tcaccgtcgg cggcgagtcg
     2761 atggaaacgt ccttggtgca ggcggctctc gagcgattgt cgggggagca gccgttcctg
     2821 ctctctggcc gttatggcag cgaccgcgcc gaggtgcggt actgggaaga agcacccgag
     2881 tgcgccgacg ccgctgcgct cgcgttgcgg ctgtggggcg agcatcgcgc gagcgcgggc
     2941 ttgcccgcat ggcatgttct tggtgtcgag gttgtcgacc gcgagatctt tcagcgccgt
     3001 ggcggtgaag gcggtgtgtc gctcatcgcg cccgccggag gttggcggcc tttctaagct
     3061 cgccgcccta tcctgggagt gccgacacgt cgaccgccta ggaggatcag ttgagcaccg
     3121 ccgctagcac cccgccgtcc ggcgatagtg ctgctgacgt gctcgctacc gcacgacggg
     3181 cccgcgtcgc agccaacgtg ctggccacca cgccgcgcgc caccaaagac agtgccctgc
     3241 aggcggttgc tgctgcgttg atcgcgcggg ctcatgaagt gcttcaagcc aacaaggtgg
     3301 acgtcgaaac tgcggccgcg gcgggtgcga gcgaatcgtt catcgaccgg ctgcgacttg
     3361 atctgcgtcg gatcgaggcg atcgcgggcg acgtggtgaa gacggccggg ctaccggatc
     3421 cggtcggcga agtcgtgcgc ggtggagtgc tcgccaacgg cctcgaactg cgacagctgc
     3481 gcgttccgct cggcgtcgtc ggcatcatct atgagggccg gccgaacgtg accgtcgatg
     3541 cggcggcatt gtgtttgaag agcggcaatg cggtgctgct gcgcggatcg tcgtccgccc
     3601 tgatgtcgaa ccaagcgctt gtcgcgatcc tgcgcgatgc catctcatcg gtcgggttgc
     3661 ctgccgatgc ggtgcagctt gttcctggtg cgggccacga gccggcgaag catctgatgc
     3721 gtgcacgcgg actcgtcgac gtcttgatcc cgcgcggtgg tgctgagttg attcgcagcg
     3781 tggtcgaaga atcgacggtg ccggtgatcg agacgggcgt cggcaactgc cacatctatg
     3841 tcgacgcgga cgccgacctc gacatggccg tggcgattct gctcaacgcc aagacgtcgc
     3901 ggccgagtac gtgcaacacg gcagagacgg tgctcgtgca tgcagatgtc gccgacgcgt
     3961 ttcttccgcg cgcactcgct gcgttgcgct ctgcgggcgt gactgtgcac ggcgacgctg
     4021 cggttgtttc gatggatggt gccgtcgtgc cggcgaccga tgccgactgg gataccgaat
     4081 acctctcact cgacatcgcg gccgccgtcg tgccatcact cgacgcggcg atcgcgcaca
     4141 tcagagaaca cggcacggct catagtgagg cgatcgtcac aacgtcgcag gccgctgctc
     4201 gcaccttcat cgcgcaggtc gacgcggccg cggtgttggt caacgcgtcg acccgattcg
     4261 tcgacggggc cgagttcggc ttcggggccg agatcggcat ctcgacacag aagttgcatg
     4321 cgcgcggacc gatgggcctg gcggaactga cgtcgacgaa gtacgtcgtg gtgggcgacg
     4381 ggcacgtccg gtgagcacga ccgggtgaac gaggccccgc ccgtcctacg accgcgcatc
     4441 ggagtgatgg gcggcacgtt cgacccgatc catcacggtc acctcgtcgc ggcgagtgag
     4501 gtggcgtcaa agttcggcct cgacgaagtg atcttcgtgc cgacgggcgc cccctggcag
     4561 aagaccgacc gccaggtctc cccggccgag gatcgctacc tgatgacggt gatcgcgacc
     4621 gcgtcgaacc cgcgattctc ggtgagcagg gtcgagatcg atcggccggg gcccacctac
     4681 acgtccgaca ccttgaccca gttgcggcgc gaacttgccg agtcgcgtgg tgcagatccg
     4741 gagatctttt tcatcacagg ggccgacgcg ctcgcccaga tcatgacctg gaacaacgcc
     4801 aacatgctgg ttgatttggc ccactttgtg ggggtgacac gaccgggcca cccactcgct
     4861 gacccccggg ggctccccga gggggcggtg agtctcgtag aaattcccgc tctggcaatt
     4921 tcttctaccg aatgtcgcga aagggtcggg cacaatcagc cgatctggta cctcgtgcca
     4981 gacggaattg tgcagtacat ctccaagcgc ggcttgtacc ggagccaggc gtgagcggca
     5041 agcacggccg cgccaagccg gggcgcagtt ccgagctgtc cggcgccgtt tccgagggcg
     5101 cccaccaagg tggcgcgtcg gtcatcgagg cgcccgccga ggtcgccccg gttcacgcgc
     5161 cgacggttgt ctacctgccg attt
//
LOCUS       DASWVF010000545         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_320022, whole genome shotgun sequence.
ACCESSION   DASWVF010000545 DASWVF010000000
VERSION     DASWVF010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320022"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VGD55_09655"
     CDS             <1..983
                     /locus_tag="VGD55_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870650.1"
                     /translation="IIGQLIVSSGISAVLDLGLMRKAEQARLVADLLDYLFTHNREPL
                     WLILEEADAFAPQQPMGELTRVLGEVDRIARRGRNFGFRLISITQRPAKLNKDVLTQL
                     STLVALGVTSPQDRDAVKAWVEGNADREQAKRVYESLARLPVGEGWVWAPDHDLLERF
                     KFPAIRTLDTSKTPKAGEARVMAPVLASADVAKLTRQIESIQAAQGAAARRRRASVAP
                     RVAVSVEQLPSARAEGPLPVQPRTGSRRRAGGALTASAPSQIARAMLAARTAAKLTQV
                     ELAARLRTSQAAILRLEKGRSRPSSRTLHRIAKATGHELVISFVKRRSPF"
     gene            1411..2634
                     /gene="istA"
                     /locus_tag="VGD55_09660"
     CDS             1411..2634
                     /gene="istA"
                     /locus_tag="VGD55_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY0870651.1"
                     /translation="MLEPEIVRQIRGLAAMSWGSKRIAATLGISRGAARRYLRGAAPG
                     AQERPGARRLDADQRAVAVTLLDGEAAGNAVVARQLLGERGIAVELRTLQRAVAPHRQ
                     ARRAAEVATVRFETAPGHQLQIDFGERRVVIGGREMRVYFFVAVLGYSRRIYVRAFLR
                     ERHDDWREGLAGAFLRFGGVTQTVLVDNARALILGRDTETGVVHVHPAFSAFCADWGV
                     APRACRPYRARTKGKTESGVGYVKRNAIAGRTFESFLALEAHLATWMIAADTRIHGTT
                     HERPLDRFEREERQALRALPARPLPVRQQRLSRRVPTDAFVDVETVRYSVPHRLVRRT
                     VEVLLGDDEVVIFDGVEVVARHRRVREPFTRVVEPGHFAGLMRPRDDIASVTPLLPAH
                     GRTLDDYAAVVAGAA"
     gene            2631..3401
                     /gene="istB"
                     /locus_tag="VGD55_09665"
     CDS             2631..3401
                     /gene="istB"
                     /locus_tag="VGD55_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY0870652.1"
                     /translation="MSEIVRARVEERLLRLRLGSVATRLDAILSQAARKEPTYLDFLD
                     EVLGDELDAKQKKRVAMAVQIAHFPTVKLLEEFDFKFQPSVDQRLVRELATGHFIAGA
                     ENVLLFGPPGVGKTHLAIGLGRAVVEAGHSVLFITATSLIGSLAKAESEGRLADQIGF
                     FAKPKLLIVDELGYLPFEKRSANLFFQLVTRRYERGSMLITTNQMVTQWGQVFGDEMI
                     AAAVLDRLLHHSHVLVVQGESYRLKQKKRAGLVARSAG"
BASE COUNT          644 a         1210 c         1231 g          627 t
ORIGIN      
        1 cgatcatcgg ccaactgatc gtctcgtccg gcatctccgc ggtgctggac ctcggcctca
       61 tgcggaaggc cgagcaggcg cgtctcgtcg ctgatctgct cgactacctt tttacgcaca
      121 accgcgagcc gctttggcta atcctggagg aagctgatgc cttcgccccg cagcaaccca
      181 tgggggagct gacgcgggtg ctcggagaag tggaccgcat cgctcgccga gggcgaaact
      241 ttgggtttcg gctgatcagc atcacgcagc gtccagcgaa gctaaacaaa gacgtgctga
      301 cacagctgtc gaccctcgtc gccttgggag tcacctcgcc tcaggatcgg gatgccgtga
      361 aagcctgggt cgagggcaac gcggatcgcg agcaggcaaa gcgcgtttac gaaagcctcg
      421 cgaggttgcc cgtcggcgaa gggtgggtgt gggcacccga ccacgatctg ctcgagcgct
      481 tcaagtttcc cgccattcgc acactcgaca cctcgaagac acctaaggcg ggcgaggctc
      541 gagtgatggc tccggtactt gcctccgccg atgtcgccaa gctcacgcga caaatcgaat
      601 ctatccaagc cgctcaaggc gccgccgctc ggagaaggcg ggcgtccgtc gctccccgtg
      661 tagcggtctc tgtcgagcag cttccatctg ctcgggcgga ggggcccctc ccggtacagc
      721 cccggaccgg gtctagacgg cgtgcaggag gggcgctaac tgcctccgct ccgtcccaga
      781 tcgcccgagc aatgttggct gcccgcacag cggcgaagct aacccaggtc gagcttgccg
      841 ccaggctgag gacgtcccag gctgctattc tgcggctgga aaaaggcaga agccggccgt
      901 cctcacgaac cctgcaccgg atcgccaagg cgacggggca cgagctcgtc attagtttcg
      961 tgaaacggcg gtcacctttt taggggggca tgaccgacga cgtcgagtga tcgatgtggg
     1021 ctccggacag gggcgagatt cggacgcaag cagagccgcg cagcaggcgc gttggaagta
     1081 ccccgaagcg ggagagcggc ggaccggtgc acgctacccg actgccctaa ccgcggaagc
     1141 ctcagccgcg cgtaagaaca gtcggacgga ttgtcagacc gggagatgcc ggccagcgaa
     1201 ctctcagaag cgcgcccacc aaactctcag aagcgcgcca aggctcccgg cgaagtcagt
     1261 tccctactcc tccggattcg atggctgccg cccgtgtcag gcgacacgta aactgacccc
     1321 caaacgacac taaaagtgac cccctccggg caggagggat cgatggacca gggacagaga
     1381 gacgtggcac tccgggcgac ggaggtgccg atgttggagc cggagatcgt gcggcagatt
     1441 cgagggttgg ccgcgatgag ctggggatcg aagcgcatcg cggcgacgct cggaatcagc
     1501 cggggtgctg cccggcggta cctgcggggt gcggcgccgg gggcgcagga gaggcctgga
     1561 gcgcgacggc tcgacgctga tcagcgtgct gtcgccgtga ccctgctcga cggcgaggcg
     1621 gctggcaatg ccgtggttgc gcgccagctt ctgggcgagc gcgggatcgc ggtggagttg
     1681 cgcactctgc agcgggcagt ggcgccgcat cgccaggctc gccgcgccgc cgaggtggcg
     1741 acggtgcgct tcgagacagc gcccggtcat cagcttcaga tcgacttcgg cgagcggcgc
     1801 gtggtcatcg gcggccgaga gatgcgggtc tatttcttcg tcgcggtgct gggctattcg
     1861 agacggatct atgtgcgggc gtttctgcgc gagcgacacg acgattggcg cgaggggctc
     1921 gccggtgcct tcctacgctt cggcggcgtc acccagacgg tgctggtcga caatgcgcgg
     1981 gcgctgatcc tcgggcgcga caccgaaacg ggcgtcgtgc acgtgcaccc agcgttcagc
     2041 gccttctgcg ccgactgggg cgtggcgccc cgcgcttgtc gtccgtacag ggcacggacc
     2101 aagggcaaga ccgagtcggg cgtcggctac gtgaagcgaa atgccatcgc tggccgtacc
     2161 ttcgagagct tcctcgcgct cgaggcgcat ctggcgacgt ggatgatcgc cgccgacacg
     2221 cggattcacg gcacgacgca tgagcgacct ctcgatcgct tcgagcgcga ggagaggcag
     2281 gctctgcgag cgttgcccgc caggccgctg cccgttcgac agcagcgtct gtcccggcgc
     2341 gtccccacgg atgctttcgt cgacgtcgag acggttcgct acagcgttcc tcatcgcctg
     2401 gttcgtagga ccgtcgaggt cctcctcggc gacgacgagg tcgtgatctt cgacggtgtc
     2461 gaggtcgtcg cgcgacatcg ccgtgttcga gagcccttca cgcgagtcgt cgagcccggt
     2521 cacttcgctg gcctcatgcg accacgtgat gacattgctt cggtgacgcc gctgttgccg
     2581 gcccacgggc ggaccctcga cgactacgct gccgtcgtcg caggtgcagc gtgagcgaga
     2641 tcgttcgcgc ccgtgtcgag gagcggctcc tccgactgcg cctcggctcg gttgccacgc
     2701 gcctcgacgc gatcctgtcg caggcggccc gcaaagagcc tacctacctc gacttcctcg
     2761 acgaggtcct cggcgacgag ctcgacgcca agcaaaagaa gcgggtcgcg atggccgtcc
     2821 aaatcgcgca tttcccgacg gtgaagctgc tcgaggaatt cgacttcaag ttccagccgt
     2881 cggtcgatca gcgattggtg cgcgagcttg ctacaggcca cttcatcgcc ggcgccgaga
     2941 atgtgctgct gttcggtcca cctggcgtcg gcaagaccca tctcgcgatc ggtctcggcc
     3001 gcgcagtcgt cgaggcgggg cactcggttc tcttcatcac ggcgacgtcg ctcatcggct
     3061 cgctcgccaa ggccgagtct gagggacgcc tcgccgatca gattggcttc ttcgcgaagc
     3121 cgaagctcct catcgtcgac gagctcgggt acctgccctt cgagaagcgc agcgccaacc
     3181 tcttcttcca gctcgtcacg cgtcgctacg agcgcggcag catgctcatc accaccaatc
     3241 agatggtcac gcagtggggc caggtcttcg gcgacgagat gatcgccgcc gctgtcctcg
     3301 accggctcct gcatcacagc cacgtcctcg tcgtgcaggg cgagagctac cgactcaaac
     3361 agaagaagcg cgccggcctg gtcgccagga gcgcggggta atcactgaac aaacgagggg
     3421 gtcattttta gtgtcgtaag ggggtcactt cccgatgtcg tttgacagcc cggccacggc
     3481 cagtccgccg acgatacctg cgaccaacgt gatgaggtag cctgcaccca gaagcggcag
     3541 cgttaccacg taaacggctc tccagtcttc ctgtcctgcg tattccacgg cgaccgatca
     3601 cgcattccag agaaattgat cagtgaaccg gtcccccgat tgggggcccg cagcggcccc
     3661 atagcggcta tccggacggt cggcatcgcg gggtcaagag cggcgcaagc cg
//
LOCUS       DASWVF010000546         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_193205, whole genome shotgun sequence.
ACCESSION   DASWVF010000546 DASWVF010000000
VERSION     DASWVF010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193205"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGD55_09670"
     CDS             complement(<1..432)
                     /locus_tag="VGD55_09670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY0870653.1"
                     /translation="MRTLAASIDLQPTKTLGQNFVIDGGTVQRIVRSAALLPDDVVLE
                     IGPGLGSLTLALLPNVRRVIAVEIDGRLAELLPQTVAARLPEFADRLTVIHRDGLTLD
                     LRGDDLPIAIVANLPYNVAVPVLLTMLARLPSVRRVLVMVQL"
     gene            complement(531..1643)
                     /locus_tag="VGD55_09675"
     CDS             complement(531..1643)
                     /locus_tag="VGD55_09675"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEY0870654.1"
                     /translation="MPPRIVVVSVYGVITAALVAGTTAFVSLDKNVTIAVDGQVTHVR
                     TYAASVDGVLHRAGVTVGPHDSLTPAPSVRVHDGTQISIARGRLVTLTVDGVTHQAWV
                     TGDNVDQVLQQAGLRIPDAANVSVSRDARVPLSGMSISIDMPHLVNVRVDGTVHEVIS
                     TQETLAGVLDQAHIVVNADDEVSTDLRTRPYDGLVLVIVRISSGQQLESAPVPFTTQT
                     VPDATMLVGTKKVEQVGSAGTMVSTYQLSYADGVQTAKNLVAQQVTLQPVSQLIGFGT
                     KPKPKPKPAPVYKIAADGLNWAALARCESGGRANAVDPPYYGLYQFRANTWHAVGGSG
                     LPSAASASEQTYRAQLLYKRSNWRTQWPVCGHNLFS"
     gene            complement(1935..>2722)
                     /locus_tag="VGD55_09680"
     CDS             complement(1935..>2722)
                     /locus_tag="VGD55_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0870655.1"
                     /translation="PAPAPLSVETVDSHCHLDAMGVPVAEALAAAQAVGVRRLVTVGD
                     TLPSSRWCAETAAAFPDVYAAVAVHPNECADFTQVDADELAQLAQLPDVRAIGETGLD
                     YYWGRVDPLVQQAVFRTHIEIAKSAGKALVIHDRDAHDDVLRILDEVGAPDRTVFHCF
                     SGDISISRHSVKRGYHLSFAGTVTFKNAHALREAASVVPLELMLVETDAPFLTPTPYR
                     GRPNAPYLIPLTVRALADVKHVDEDAVASATAVNAQRVFGSWR"
BASE COUNT          497 a          866 c          893 g          466 t
ORIGIN      
        1 gagttgcacc atgacgagca cccggcgtac cgaaggcaat cgggccagca tggtcaacag
       61 caccggcacc gcgacgttgt acggaaggtt cgcgacgatc gcgatcggca ggtcgtcgcc
      121 gcgcaagtcg agcgtcagcc cgtcacgatg aatgaccgtc agccggtcgg cgaactcagg
      181 cagccgcgcg gcgacggtct gcggcaatag ctcggcgagc cggccatcga tctcgacggc
      241 gatgacgcgc cgcacgttcg gaagcagcgc caaggtcagg gagccgagcc caggcccgat
      301 ctcgagcacg acgtcgtcgg gaagaagcgc cgcactgcga acgatccgct gcactgtgcc
      361 gccgtcgatg acgaagttct ggccaagggt cttcgtaggc tgaaggtcga tcgacgcggc
      421 gagcgtgcgc acatcagttg caccgagcag cgcacctgcc ggcgtcgctt ccccccggtc
      481 tcgcgctacg tgtcgccctt gggcttttgc tgtggagtct tctgacgtgt tcaggagaac
      541 aggttatggc cgcacacggg ccactgtgta cgccagttgc tgcgcttgta gagaagctgt
      601 gcgcggtagg tctgctcgct ggccgacgca gcgctcggca gaccgctgcc gccgacggcg
      661 tgccaggtgt tcgcccggaa ctgatagagc ccgtagtagg gcgggtcgac ggcgttcgcc
      721 cggccgcctg attcgcaacg cgccagcgca gcccagttca ggccgtcggc ggcgatcttg
      781 tagacgggtg ccggcttggg cttcggcttg ggcttggtac cgaagccgat gagctgcgag
      841 accggctgca gcgtcacctg ctgcgccacc aggttcttag ccgtttgcac gccgtcggcg
      901 tagctgagct gataggtgct caccatcgtg cccgcgctgc ccacctgctc gaccttcttg
      961 gtaccgacga gcatcgtggc atcgggcacc gtctgggtcg tgaacgggac cggcgcgctc
     1021 tcgagctgct ggccgctgct gatgcgcacg atcacgagga ccagtccgtc gtagggccgc
     1081 gtccggaggt cggtcgagac ctcgtcgtcg gcattgacca cgatgtgggc ctggtccagc
     1141 acgccggcta gcgtctcttg cgtactgatg acttcgtgca ccgttccgtc gacgcgcaca
     1201 ttcacaagat gcggcatgtc gatcgagata ctcatgcccg acagcggaac tcgtgcatca
     1261 cgggagaccg acacgttcgc agcatcagga atacgaagac ccgcctgctg caatacctgg
     1321 tcgacattgt cgccagtgac ccatgcctga tgcgtaacac cgtcgacagt aagtgtgacc
     1381 aaccgaccgc gcgcaatcga aatctgcgtg ccgtcatgaa cgcgcaccga gggtgcgggc
     1441 gtcaatgagt catgcggtcc gacggtaacg ccggcgcgat gcaaaacacc atcaacagat
     1501 gcagcgtacg tgcggacatg cgtgacctga ccgtcgaccg caatggtcac gtttttgtcg
     1561 agactgacga acgccgtcgt ccccgctacc agggcagcgg tgatgacgcc gtacaccgaa
     1621 acgacgacga tccgaggtgg cacaggactc cgctgttcga agggtccaga ggacgggaag
     1681 tgtggcgcgc gcagcgcagc tgggtcgaaa agcgatcccg ggacgccgac cacaagcttc
     1741 cccgctctgg ccgaacactg acgtgaacgt aacgaaccag tagttgagga agcaagcgat
     1801 cggtggttcg gggtaccgcg cttggtgtcg atttgccctg ctcttaatgg agttcgcccg
     1861 gtacggcagg acggccgcca ccggggctgt ggcggctgtg acttctgtga ccgggccgct
     1921 gcccagaccc tggcctagcg ccaactgccg aatacccgct gcgcgttgac cgctgtcgcc
     1981 gacgcgactg catcttcgtc gacatgcttc acgtcggcga gcgcgcgcac cgtcaacggg
     2041 atgagatacg gcgcattcgg ccggccgcgg tacggcgtcg gtgtgaggaa cggagcatcg
     2101 gtctcgacga gcatcaactc gagcggcacg acagatgctg cttcgcgaag tgcatgcgcg
     2161 ttcttgaacg tcactgttcc ggcgaacgac agatggtacc cgcgcttgac actgtgtctc
     2221 gatatcgaga tatctccgga gaagcagtgg aacactgttc gatcgggtgc accgacctcg
     2281 tcgagaatcc tcaacacgtc atcgtgcgca tcgcgatcgt gaatcaccaa cgccttgccc
     2341 gcgctttttg cgatctcgat gtgagtgcga aagacagctt gttgcacgag cggatcgacc
     2401 cgcccccagt agtagtcgag tccggtttct ccaatggccc ggacgtcggg aagctgggcc
     2461 agttgcgcca actcatcggc gtccacctgg gtgaagtcgg cacactcatt ggggtgtacg
     2521 gccaccgcgg catagacgtc gggaaaggcc gccgccgtct cggcacacca ccgcgacgac
     2581 ggcagggtgt cacccacagt gaccaggcgg cggacgccga cggcctgggc ggcggccaaa
     2641 gcctcggcga ccggcacccc catcgcgtcg agatggcagt gcgaatcgac ggtttcgacg
     2701 ctcaggggcg ccggcgccgg cg
//
LOCUS       DASWVF010000547         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_218920, whole genome shotgun sequence.
ACCESSION   DASWVF010000547 DASWVF010000000
VERSION     DASWVF010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218920"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGD55_09685"
     CDS             <1..882
                     /locus_tag="VGD55_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870656.1"
                     /translation="ATTTDPAVLWAIQNGPSKLYRLLWNSTTNTWESDALNSWATGKT
                     IHYLNGLGDPDSEGVTKAEFADSSIYVSTERDNDANTINRFSVLRYDTTAAGTDLTAV
                     NDWELTADLPATGINLGLEAITYVPDAALVSGHFFDEAANAVYDPARYPNHGTGLFFV
                     GLESNGVIYGYALNHADNSFTRVATFASGQVAIMDLSYDREVGYLWAQCDNTCGNKAT
                     VLRLDTAAMSPTAGRFTIGRVFDRPASLPNSNFEGIGIAPEAECAGGQKHFFWSDDNA
                     LDMHSLRRGSIPCGAFF"
     gene            complement(879..>1975)
                     /locus_tag="VGD55_09690"
     CDS             complement(879..>1975)
                     /locus_tag="VGD55_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEY0870657.1"
                     /translation="FREKPSINDLIMTPMGGAVIGEATYRVGRMFAAGRPSIPNCIGA
                     MVFSLVATLNETSVCRSTSRPPYDGVGLSQSTPHRLDVAVGESFARFDGGPLSGQLLI
                     DFAAVVVTNPACNRPGHETVPVHAGQWTSLIVESLLSEGNLPGFWLHTDMVWWGRYAR
                     HFGEPSGEQGDRPHGHGLLLALDSTFDYDTRDLPAEKDRTATAGILGPRLEYTSRRGR
                     VTWRAELGVEYGFSIVTSLVYPFAAPTLAGQEIKSELWQQGYYYAQSVVARAGVQARI
                     GTVELSAAARLMDAWSFDRGDRYQGEITNNFSLFDQRTWVRTAVTVPIFGGPLNAMLM
                     AERDDRSSHIPVFSVHSVETRGALSVLARF"
BASE COUNT          352 a          703 c          616 g          304 t
ORIGIN      
        1 gcgacgacca ccgaccccgc cgtgctgtgg gccatccaga acggcccctc caagctgtac
       61 cgcctgctct ggaacagcac gacgaacacc tgggagtctg acgccctcaa cagctgggcc
      121 accggcaaga ccattcacta cctgaacggg ttgggcgatc ccgattccga gggagtcacc
      181 aaagccgaat tcgcggacag ctcgatctac gtgtcgacgg agcgcgacaa cgacgcgaac
      241 accatcaatc gcttcagcgt gcttcggtac gacaccaccg ccgccggcac ggacctgacc
      301 gcggtcaacg actgggagct caccgccgat ctgccggcca ccggaatcaa cctgggcctg
      361 gaagcaatca cgtacgtgcc cgatgcggcg ctggtgtcgg gacacttctt cgacgaggcg
      421 gcgaatgcgg tctacgaccc ggcgcggtac ccgaatcacg gcacggggct gttcttcgtc
      481 ggactcgagt cgaatggcgt catctacgga tacgcgttga accacgccga caacagcttc
      541 acccgggtcg cgacgttcgc cagcggccag gtggcgatca tggatctgtc gtacgaccgc
      601 gaggtcggct acctgtgggc ccagtgcgac aacacgtgcg gtaacaaggc caccgtgttg
      661 cggctggaca ccgccgcgat gtcgcccacg gccggccgct tcacgatcgg ccgcgtcttc
      721 gaccgcccag cctcgttgcc gaactcgaac ttcgaaggca tcggcatcgc gccagaggcc
      781 gagtgcgccg gcgggcagaa gcactttttc tggtccgacg acaacgccct cgacatgcac
      841 tcgctccgcc gcggctccat cccctgcggc gcgttcttct agaagcgagc cagcaccgaa
      901 agcgcgccgc gcgtctcgac gctgtggacg ctgaacacgg ggatgtggct gctgcggtcg
      961 tcgcgctccg ccatcagcat cgcattcagc ggaccaccaa agatcggcac ggtcaccgcc
     1021 gtccgaaccc aggtacgctg atcgaaaagc gagaagttgt tggtgatctc cccctgatag
     1081 cggtcgccgc ggtcgaatga ccaggcgtcc atgagacgcg ccgccgccga gagctcgacg
     1141 gtgccgatgc gcgcctggac gccggcgcgc gccaccacgc tctgcgcgta gtagtagccc
     1201 tgctgccaga gctcgctctt gatctcctgc ccggccaacg tgggcgccgc gaagggatag
     1261 accagcgagg tcacgatcga aaagccgtat tcgacgccca gctccgcgcg ccaggtcaca
     1321 cgcccgcgac ggctcgtgta ctcgagccgc ggtcccagga tcccggcggt cgccgtgcgg
     1381 tccttctccg ccggaaggtc ccgcgtgtcg tagtcgaacg tgctgtccag cgccaacagc
     1441 aggccatggc cgtgcggccg gtcgccctgc tccccgcttg gctcgccgaa gtgccgggcg
     1501 taccggcccc accacaccat gtcggtgtgc aaccagaagc cgggcaggtt tccctcagac
     1561 agcaggctct cgacaatcaa cgacgtccac tgtcccgcgt gcaccggcac cgtctcgtgg
     1621 cccggccggt tgcacgcggg gttcgtgacg accaccgccg caaaatcgat gagcagctgt
     1681 ccgctgagtg gaccgccgtc gaagcgcgcg aaggattcgc caaccgccac atccagtcgg
     1741 tgcggcgtgc tctgcgacag cccgaccccg tcgtacggcg ggcgcgacgt ggatcgacac
     1801 accgacgtct cgttgagcgt cgcgaccagc gagaacacca tcgcgccgat gcaattgggg
     1861 atgctgggcc ggccggcggc gaacatgcgc ccaacccggt aggtcgcctc cccgatgacg
     1921 gcgccgccca tcggcgtcat gatgaggtcg ttgattgacg gcttctccct gaact
//
LOCUS       DASWVF010000548         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_116874, whole genome shotgun sequence.
ACCESSION   DASWVF010000548 DASWVF010000000
VERSION     DASWVF010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116874"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VGD55_09695"
     CDS             complement(<1..1106)
                     /locus_tag="VGD55_09695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0870658.1"
                     /translation="MAARVRMDTELADPRPSRSLRASVIERLRSSGGVAQFLVVGVLF
                     ACSAVAVGLYVFTAFHRMRFRFELEWMEGGILEHVKRVLSGKTVYPAPSVDFAAFIYN
                     PLYYYVAAPFCAVFGLAMFPLRLVSFLASVVSFLLLCLSVKKHTGKLLAGVLATGLFA
                     GTFAASGAWFDLARIDSLGLALVLAAGYLLIGPRDRPGDVSGRALIASGALAALAILT
                     KQSAAFVVPVLLLRAFTIGRFRGAALFGGALLLPLGFAVLALQIQSHGWYWYYAFQLP
                     STHTIAGREPLVLDFWRYELFAALPIAATTALLELCCLPITGASRNWFVPAFAVTLVL
                     VSYVTRLHMGSYTNDLMPAHAGLAILSTTLLSRLA"
     gene            complement(1148..2617)
                     /gene="ahcY"
                     /locus_tag="VGD55_09700"
     CDS             complement(1148..2617)
                     /gene="ahcY"
                     /locus_tag="VGD55_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0870659.1"
                     /translation="MAEISTFKRAPVVKGKPDYVVRDLSSAAFGRKEIDLAETEMPGL
                     VATREEFAKSQPLAGARIAGSLHMTIQTAVLIETLEALGAEIRWCSCNIFSTQDHAAA
                     AIADRGVPVFAVKGESLEEYWDYSWRVLQWSDGGTPNLILDDGGDLTLLVHLGVKAEK
                     DPSFLEQDPGHEEGRVLYALVKRAIKERPGFFAQAAKNIKGVSEETTTGVHRLYQMAQ
                     KKELLFPAINVNDSVTKSKFDNLYGCRHSLPDGIMRATDVMLSGKVVVIAGYGDVGKG
                     CAQAMKGQGARVLITEIDPICALQAAMEGYEVTTMTKAAPRGDIFVTTTGNEFVITEA
                     HMNAMKHRAIVCNIGHFDSEIEISKIKKHKWVEVKPQVHEVIWPDGKSIIVLAEGRLV
                     NLGCATGHPSFVMSNSFTNQTIAQIDLWLASQRKPTLSGVAGYEPGRVYTLPKKLDEK
                     VARLHLAKLGVELEELTDRQASYLGIPKEGPYKADHYRY"
     gene            complement(2750..3148)
                     /locus_tag="VGD55_09705"
     CDS             complement(2750..3148)
                     /locus_tag="VGD55_09705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870660.1"
                     /translation="MSRAELLHEIEAGVTFWGYADDGEMLGIMGLQDVRDVTLIRHAY
                     VRTANQRCGIGALLLGHLRQLTARPILIGTWAEAPWAIRFYRRHGFEPVGREKKERLL
                     RRCWTIPDRQIETSVVLADDRWRASHLRLT"
     gene            3206..3688
                     /locus_tag="VGD55_09710"
     CDS             3206..3688
                     /locus_tag="VGD55_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870661.1"
                     /translation="MRSSSSCARSSCRRSRATRKRSPPRPRARAGPSASTSSTLQSSR
                     SRSAASAVSTAPCVTPTCHRRKRLATLDRARLPNKVTKQLPALCDGGFVERGDNLLVF
                     GLPGRGKTHVVAAIGHELVRRGYRVLFTPTFALVQRLLAAKRDLRLERELVSLDTYPL
                     "
     gene            complement(3843..>4482)
                     /locus_tag="VGD55_09715"
     CDS             complement(3843..>4482)
                     /locus_tag="VGD55_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870662.1"
                     /translation="KPCNDNTWGCCCNENPQYENQHITKQFGGDPSITYHVKLRLAGV
                     SERYWYTGGTLDPVSKLFYAGGLPTVNGPPPGTTPRPSACKIQPPKTDGPPNNFALGF
                     TVPTEVNPSDGCFNGFNIFAMTVSSPKKSYYLNYTQDFDGTDRQPHACYENDYTVTVP
                     IAGQAQLDFYVIDGDHHQVANTGTQMVPNVKTKQPYHGNFWEFQVLDVTTGD"
BASE COUNT          816 a         1492 c         1382 g          792 t
ORIGIN      
        1 gccaagcggg acagcagggt cgtgctcaag attgccaatc cagcatgcgc cggcatcaga
       61 tcgttcgtgt agctccccat gtggagccgc gtcacgtacg acaccagcac cagcgttacg
      121 gcgaatgccg gaacgaacca gttcctgcta gcgcccgtga tcggcagaca gcacaactcc
      181 aacaaagcgg tcgtggctgc aatcggcaac gcggcgaaca gctcgtatcg ccaaaaatcg
      241 aggaccagtg gctcacgtcc ggcgatggtg tgagtcgacg ggagctgaaa cgcgtagtac
      301 cagtaccacc cgtgcgattg aatttgcagc gcgagcacgg cgaagcccag cggtagcaac
      361 aacgcgccac cgaacagcgc cgcgccgcgg aatcggccga tcgtgaacgc gcgcaacagc
      421 aggactggca cgacgaacgc ggcgctctgc ttcgtgagga tggcgagcgc ggccagcgcg
      481 cccgacgcga tcagggcacg gccgctgacg tcgcccggcc ggtcgcgcgg accgatgagt
      541 agataaccgg ctgccaacac caacgcgagg ccgagcgagt cgatgcgcgc gaggtcgaac
      601 caggccccgc ttgccgcgaa cgtgcctgca aagagcccgg tggcgagcac accggccagg
      661 agtttcccgg tgtgcttctt gacggagaga cagagcagca gaaacgagac aacagaggcc
      721 aggaacgaca ccagccgcag cggaaacatc gcgagcccga acaccgcaca gaacggagcc
      781 gcgacgtagt agtagagggg gttgtagatg aaggcggcga aatccacgga cggcgccggg
      841 taaaccgtct tgccggagag cacccgcttc acatgctcca gaattccccc ctccatccac
      901 tccagctcaa accggaagcg catccggtgg aaggcagtaa agacgtagag cccgacagcc
      961 accgcgctgc acgcaaagag aaccccgacc accaggaact gcgcgacccc acccgaagag
     1021 cgcaacctct cgatcaccga cgcacgaagc gatcgagagg gtcggggatc ggcgagttcg
     1081 gtatccattc gaacgcgtgc cgccatcggc gccgccttgc aaggcggcgc cgacggtcgg
     1141 gatcggtcta gtagcggtag tgatcggcct tgtacgggcc ctctttgggg atgcccaggt
     1201 aggaggcctg gcggtcagtg agctcctcca gctcgacgcc cagcttcgcc aggtgcaggc
     1261 gcgcgacctt ctcgtccagc ttcttcggca gcgtgtagac acgccccggc tcgtagccag
     1321 ccacgcccga gagcgtgggc ttgcgctgcg acgccagcca cagatcgatc tgcgcgatcg
     1381 tctggttcgt gaacgagttg ctcatcacga agctcgggtg gccagtcgcg cagcccaggt
     1441 tcaccagacg cccctcggcg agcacgatga tcgacttgcc gtccggccag atcacctcgt
     1501 gcacctgggg cttcacttcg acccacttgt gcttcttgat cttcgagatc tcgatctccg
     1561 agtcgaagtg gccgatgttg cacacgatcg cgcggtgctt catcgcgttc atgtgcgcct
     1621 cggtgatcac gaactcgttg ccggtcgtgg tcacgaagat gtcgccgcgg ggcgcggcct
     1681 tggtcatcgt cgtgacctcg tacccctcca tcgctgcctg cagcgcgcag atcggatcga
     1741 tctcggtgat caggacacgc gcgccctgcc ccttcatggc ctgggcacac cccttgccga
     1801 cgtcgccgta acccgcgatg acgacgacct tgcccgacag catgacgtcg gtcgcgcgca
     1861 tgatgccgtc cggcagcgag tgacggcagc cgtagaggtt gtcgaacttg gacttggtga
     1921 ccgagtcgtt cacgttgatg gccgggaaca gcaactcctt cttctgggcc atctggtaca
     1981 ggcggtgaac gccggtcgtc gtctcctcgg agacgccctt gatgttcttg gcggcctgcg
     2041 cgaagaagcc cggccgctcc ttgatcgcgc gcttgaccag cgcgtaaaga acccgcccct
     2101 cttcgtggcc gggatcctgc tccaggaagg acggatcctt ctcggctttg acgcccaggt
     2161 gaaccagcaa cgtgaggtca ccgccgtcgt ccaggatcag gttcggcgta ccgccgtcgg
     2221 accactgcag gacgcgccac gagtagtccc agtactcctc caggctctcg cccttgaccg
     2281 cgaacaccgg cacgccacga tcggcgatcg ccgcggcggc gtgatcctgg gtgctgaaga
     2341 tgttgcacga gcaccagcgg atctcggcgc ccagcgcctc gagcgtctcg atgaggacgg
     2401 ccgtctggat ggtcatgtgc agcgagccgg cgatgcgcgc gcccgccagc ggctgggact
     2461 tcgcgaactc ctcgcgtgtg gcgaccaggc ccggcatctc ggtctcggcc aggtcgatct
     2521 ccttgcgccc gaaggccgcc gacgacaggt cgcggaccac atagtcgggc ttgcccttca
     2581 cgacgggcgc tcttttgaac gtgctgatct cggccatctc tatctccttg ttgtcctgaa
     2641 acgcgaccta ctatatccca ttattcgtat taacggatgt ggaattcgat attaccagat
     2701 ccccctgacc tctgcccgat ctacccagcg cgaccgtggg gtgcctgact tatgtcagcc
     2761 gcagatggct cgcgcgccat cggtcgtcag caagaacaac ggacgtctcg atctgtcggt
     2821 cgggaatcgt ccaacaccgc ctcagcagtc gttccttctt ctcgcggccg acgggctcaa
     2881 agccatgacg cctgtagaac cggatggccc agggcgcttc ggcccatgtt ccgatgagga
     2941 tcggccgagc cgtcaactgc cgcaaatgtc caagtaggag cgcgccgatg ccgcaccgct
     3001 ggttggcggt ccgcacgtat gcgtgccgaa tgagggtgac atcccgaaca tcctgcagac
     3061 ccatgatgcc aagcatttcc ccgtcgtccg cataacccca gaacgtgacg cccgcctcga
     3121 tctcgtggag tagctccgcg cgcgacatgt acggactacg cagcaggtcg ggaggaattg
     3181 cgccgcgata agccgagcgc cctcgatgcg ctcctcgtcc tcttgcgcgc gctcaagctg
     3241 ccggcgttcg cgcgctactc ggaagagatc gcctccaagg ccacgcgcga gggctggacc
     3301 ttcggccagt acctccagca ccttgcagag ctcgagatcg aggagcgccg cgtccgccgt
     3361 atcgaccgcg ccctgcgtga ctccgacctg ccatcggaga aaacgtctcg ccaccctcga
     3421 tcgcgcgcgc ctgccgaaca aggtgacgaa gcagcttccg gccctgtgcg acggcggctt
     3481 cgtcgagcgc ggcgacaatc tcctcgtctt cgggctgccc ggtcgaggca agacccacgt
     3541 cgtcgccgcc atcggccacg agctcgtccg tcgtggctac cgcgtgcttt tcacgccgac
     3601 cttcgccctc gtccagcgac tcctcgccgc caagcgcgac ctgcgcctcg agcgcgagct
     3661 cgtctcgctc gacacctacc cgctgtgatc ctcgacgaca tcgggtaccc gctcgtcaag
     3721 cctgagcacg cgtaggacgt gcgagtccac cggttcgtcc atcgcgacac ccaaatgaag
     3781 tcagcggctc acatcggccg gcaaacgaca gagctctcca ccggtcggtc atgatggttc
     3841 gctcagtccc cggtggtgac gtctagaacc tggaactccc agaagttgcc gtgatagggc
     3901 tgcttggtct tcacgttggg aaccatttga gttccggtgt tggccacctg atggtggtcg
     3961 ccgtcgatca cgtagaagtc gagctgcgct tgtccggcga tcggcaccgt cacggtgtag
     4021 tcgttctcgt aacaggcgtg cggttgccgg tccgtgccgt cgaagtcctg ggtgtagttc
     4081 aggtagtagc tcttcttcgg cgacgacacc gtcatcgcga agatgttgaa cccgttgaag
     4141 cagccgtcgc tcggattgac ctcggtcggc acggtgaagc ccagcgcgaa gttattcggc
     4201 ggaccatccg tcttgggcgg ctggatcttg cacgccgacg ggcgcggagt cgtcccagga
     4261 ggagggccat tcaccgtcgg caggcctccc gcgtagaaga gtttgctcac tgggtccagt
     4321 gtcccgcccg tgtaccagta gcgctccgat acaccggcga ggcggagctt gacgtggtac
     4381 gtgatgctcg gatcacctcc gaactgcttc gtgatgtgtt ggttctcgta ctgcgggttt
     4441 tcattgcagc aacaacccca cgtgttgtcg ttgcacggct tg
//
LOCUS       DASWVF010000549         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_167548, whole genome shotgun sequence.
ACCESSION   DASWVF010000549 DASWVF010000000
VERSION     DASWVF010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167548"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2465)
                     /locus_tag="VGD55_09720"
     CDS             complement(<1..>2465)
                     /locus_tag="VGD55_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0870663.1"
                     /translation="GRAALDVVAAVSQLGVELGVPELAARAGVVTGEVAVTVGGTTEG
                     MVAGDAVNTASRVQAAARGGTVLVDAATRRLALTAVGFDDVGEFLLKGKAEPEQLWLA
                     VRVLAGVGGAQRVDGLEAPLTGRDPELRTIKELFHAAAERRVPRMVVVSGPAGVGKSR
                     LGWEFEKYIDGLADFVLWHRGRCLSYGEGLAFSALVEIVRQRFAIADEDPPAIAATKL
                     AEGLVRFVPDPVEQGYVGARLARLLGLPLAGDDGAALSRDELFAGWRLFFERMAATEP
                     VALLIEDAHYADAGLLDFCDQLVDWARDLPIFVLVFGRPELELLRPGFGTGRNRTTLT
                     LDPLDTASMGLLVDALVPGMPADARTAITLHAQGIPLFAVETVRSLVDQDIVVPREGV
                     YRLVGDVGELTVPNSLHSLLAARLDALEPAARELIADAAVLGSTFPAEALVATSGKPD
                     AQVRAVLADLLQREVLEVSADKLSPQRGTYRFAQEMLRQVAYDTISRRDRKARHLTVA
                     AYLRATFPGDGDEVMAVVARHYLDALAAVPDDPDIPQLRSLAIAALVRAGERSQRAGA
                     QAPAAASYAAAAECIIEQPQAHKGDLSAAVMFERAAEAAIFAIAYADAERYGARARQL
                     YLEDGQPRAAARAQATAGQALRRAGRHTEARELFNQAITVLRPDPDADTVTALIELAT
                     TEVFAGGPASQALADEALALGEALDVEPALLSRLFTVGGFAHAFAGHRAQATAYLRES
                     TRLAERANDVAALARSYLNLSDVLTATDAPAAADAARIAAEHARRNGDREMLAVAITN
                     LVGALVAMGEWSAADEVLHAGAD"
BASE COUNT          413 a          843 c          844 g          365 t
ORIGIN      
        1 tcgtctgcgc cggcgtgcag cacctcgtcg gccgccgacc actcccccat cgcgaccagc
       61 gccccgacga ggttcgtgat cgcgaccgcg agcatctcgc ggtcgccgtt tcggcgggcg
      121 tgctcggcgg cgatgcgcgc cgcgtcggcg gctgcaggcg catcggtcgc ggtcaggaca
      181 tcggagaggt tcagatacga acgggcgagt gcggcgacgt cgttggctcg ctcggccagc
      241 ctggtggatt cccgaaggta ggcagtggcc tgtgcgcggt gccctgcgaa ggcgtgcgcg
      301 aatccgccga ccgtgaaaag cctcgagagc aaggccggtt cgacgtcgag tgcctcgccc
      361 aatgcgagcg cctcatcagc cagcgcctgc gatgccggcc caccggcaaa tacctccgtg
      421 gtggcgagtt cgatcaatgc ggtcacggtg tcggcgtcag ggtcaggacg caacaccgtg
      481 atcgcttggt tgaagagttc gcgtgcctcg gtgtgccgcc cggctcggcg cagcgcctga
      541 ccggcggtgg cctgggcccg agcggccgcc ctcggttggc cgtcctcgag gtagagctgg
      601 cgggcgcggg cgccatagcg ctcggcatcg gcgtaggcaa tggcgaagat cgcagcctca
      661 gcggcccgct cgaacatcac cgccgcactc agatctccct tgtgagcctg cggttgttcg
      721 atgatgcact cagctgcggc cgcgtagctc gcagccgcag gcgcctgggc accggcccgc
      781 tgcgaccgtt caccggctcg caccaatgcg gcgatggcaa gcgatcgcaa ctgcggaatg
      841 tcggggtcgt caggcacggc cgcgagcgcg tcgaggtagt ggcgggcaac cacggccatg
      901 acttcgtcac cgtcaccggg aaacgtcgcc cgcaaatagg cggcaacggt gagatgtcgt
      961 gccttgcggt cgcgccgcga gatcgtgtcg tatgcaacct gtcgcagcat ctcttgggcg
     1021 aaccggtagg tgccgcgttg gggtgacaac ttgtcggcac tgacctcgag cacctcgcgc
     1081 tggagaaggt cagccaacac ggcgcgcacc tgggcgtcgg gttttcctga cgttgccacc
     1141 agtgcctcag cgggaaaggt cgacccgagc accgcggcgt ccgcgatcaa ctcacgagcc
     1201 gccggctcga gggcgtcaag acgcgctgcg agcagtgagt gcaagctgtt cggcacggtc
     1261 aactcaccga catcgcctac cagccggtag acaccttcgc gcgggacgac gatgtcctgg
     1321 tcgaccagcg accgcaccgt ctcgaccgcg aacagcggga ttccctgcgc gtgcagggtg
     1381 atcgcggtgc gcgcgtcggc aggcatgccg ggcaccagcg cgtcgaccag caggcccatc
     1441 gaggctgtgt cgagggggtc gagggtcaac gtcgtgcgat tgcgcccggt gccgaacccg
     1501 ggccggagta gttcgagttc gggccggccg aagaccaaca cgaagatggg tagatcgcga
     1561 gcccagtcga ccaactgatc gcagaagtcc aagagcccgg cgtcggcgta gtgcgcatct
     1621 tcgatgagca acgccaccgg ctcggtagcg gccatccgtt cgaaaaagag tcgccagccg
     1681 gcaaagagct cgtcccggct aagtgccgcc ccgtcgtcgc cggcgagcgg caagccgaga
     1741 aggcgggcga gccgggcgcc gacgtagccc tgctcgactg gatctggaac gaatctcacg
     1801 agaccctcag cgagcttcgt cgcggcgatc gctggcggat cctcgtcggc gatagcgaat
     1861 cgctgccgca cgatctcgac gagcgccgag aacgcgagcc cttcaccgta ggaaaggcag
     1921 cggccgcgat gccacaacac aaagtcggcc aggccgtcga tgtacttctc gaactcccaa
     1981 ccaagccgcg acttgcctac gcccgccggg ccactcacca cgaccatgcg aggcactcga
     2041 cgttcggcgg cggcgtggaa cagttccttg atggtgcgca gttcagggtc gcgaccggtg
     2101 agcggcgcct cgaggccgtc gacacgttgc gccccgccca cgccggccag cacccgaacc
     2161 gcgagccaca gttgctcggg ttcggccttg cccttcaaca agaattcgcc gacgtcatcg
     2221 aagccgaccg cggtcaaggc aaggcgcctg gtcgcggcat cgacgagcac ggtgccgccg
     2281 cgtgcggcgg cctgcacgcg cgaagcggtg ttgacggcgt cgcccgccac catcccttct
     2341 gtggtgccac caacggtcac ggcaacttcg ccggtcacca ccccggccct cgcagcgagt
     2401 tcgggaacgc cgagttcgac gccgagctgg ctgaccgctg cgacgacgtc gagggccgcg
     2461 cgtcc
//
LOCUS       DASWVF010000550         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_193448, whole genome shotgun sequence.
ACCESSION   DASWVF010000550 DASWVF010000000
VERSION     DASWVF010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193448"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGD55_09725"
     CDS             <1..664
                     /locus_tag="VGD55_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870664.1"
                     /translation="PIGGTVYLLGGNSALAPAVASSVAALGFTTVRIAGPDRFTTAAL
                     VAAELGNPVTVFEADGTNFPDALSAGTAAASVGGAILLTAGSSQSPATASYLAAHPGD
                     KRYAIGGPAASADPGATAYVGADRFATSVAVAEAFFPAPGSIGLASGFTFPDALSGGS
                     VAAMNHGPIVLVPSTGTLPVTVASYLATAKASATAAWLFGGTSSVSADVFNQAASLLT
                     AP"
     gene            666..1259
                     /locus_tag="VGD55_09730"
     CDS             666..1259
                     /locus_tag="VGD55_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870665.1"
                     /translation="MDDDQSAGDADVLITGAGEGRSRVPRLAWIAFCAAIVLALVAGI
                     ANSTDRGSRHREFNALLGATIDGQATAVHADDLVESTRSYTMPLLGSAQPNVRAGLQQ
                     LIAQSATEGAKEVRASRAKVASTSVLPWHSSLHHAKAAELSYLDAWAAYLDSVAQGGD
                     TGAIPTAALALKRTDASAALHSAAPDRTASLAIPSSL"
     gene            1256..>2159
                     /locus_tag="VGD55_09735"
     CDS             1256..>2159
                     /locus_tag="VGD55_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870666.1"
                     /translation="MIRNRPTGPHGRNGDDGVDSVDGLDTVVRATALRILNRAWDPAG
                     GYCRPNRRSYPHLWMWDSCFHAIAWFALGDERGTRELEAIFAGQLPNGFLPHMRYGRR
                     TYARGPLKAVSSFTQPPVYARALKAGADAGTAPSDHLLDCAKRALEALWRDRERDGLL
                     VIVHPWEAGTDDSPRFDSWVGSSTWSRRVWTAFDRSLLKRTVFSPEGQAIDNPGFVVA
                     PASFNAIAADAAVTLGELLGDADWIARGRALADALDDAAWDADQSMWRDVAFTGGGDS
                     VQIPTLDATLPAMCTADSAKSSAAL"
BASE COUNT          340 a          733 c          715 g          371 t
ORIGIN      
        1 cccgattggc ggcaccgtgt acctccttgg agggaactcc gctcttgctc ccgcagtggc
       61 aagcagcgtc gcagcgctcg ggttcaccac cgtgcgcatc gccggccctg accgctttac
      121 gacggccgcg ctcgtcgcgg ccgagctcgg aaatccggtc acggtgttcg aggccgacgg
      181 cacgaacttc cctgacgcac tctcggccgg tacggcggct gcctcggtgg gtggggcgat
      241 cctgttgacc gccggctctt cgcaatcgcc ggctaccgcg tcgtatctcg ctgctcaccc
      301 aggtgacaag cgttacgcaa tcggcgggcc cgcggcctcc gctgaccctg gtgcaacggc
      361 ctacgtcgga gccgaccggt tcgccacctc cgtcgccgtc gccgaagcgt tcttcccggc
      421 accgggatca atcggcttgg ccagcggttt caccttcccc gacgcgctct caggtggcag
      481 cgtggcggct atgaaccacg gtccgatcgt cttagtgccg agcactggaa cgttgccggt
      541 caccgtcgcg agctatctcg cgacggcgaa ggcgagcgca actgcggctt ggctgttcgg
      601 cggcaccagt tcggtgagcg ccgatgtgtt caaccaagcg gcgagcttgt tgacggcgcc
      661 ctgagatgga cgacgatcag tccgcgggtg acgccgacgt cctgatcacc ggggctggtg
      721 aaggccggag ccgggtgccg cggcttgcgt ggatcgcgtt ctgcgccgcg attgtgctcg
      781 cccttgtcgc aggcatcgcc aacagcaccg accgtggttc gcggcatcgc gaattcaacg
      841 cacttctcgg cgccacgatc gacgggcagg caaccgccgt gcacgccgat gacttggtcg
      901 aatcgacgag gtcatacacg atgccgttgc tcggttctgc gcaacccaat gttcgcgccg
      961 gacttcaaca actcatcgcg caatcggcga ccgaaggtgc gaaggaagtg cgagcgagcc
     1021 gcgcgaaggt ggcgtctacg tccgtgctgc catggcacag ttcgctgcac catgcgaaag
     1081 cggccgagct cagctacctc gatgcgtggg ctgcctacct cgacagcgtc gcgcagggcg
     1141 gcgacaccgg ggcaattccc accgcggcac tcgccctgaa gcgcactgac gcgtccgccg
     1201 cgttgcacag cgcggcgccc gaccgtactg cttcgctcgc gatcccctcg tcgctttgat
     1261 ccgcaaccgc ccgacggggc ctcatgggcg caacggggac gacggagtcg acagcgttga
     1321 cggtctcgac acggtggtgc gcgcgactgc gctgcgaatt ctcaaccggg cgtgggatcc
     1381 agcgggcgga tactgccgac ccaaccggcg cagctacccg catttgtgga tgtgggactc
     1441 gtgttttcac gccatcgcgt ggttcgcgtt aggcgacgaa cgcggcaccc gcgagctcga
     1501 ggcgatcttc gcgggccagt tgccgaacgg attcctgccg catatgcggt acggccgaag
     1561 gacctatgcc cggggaccgc tgaaggcagt gtcgtcgttc acgcagccgc cggtttatgc
     1621 ccgtgcattg aaagcagggg cggacgccgg caccgcgccg tccgaccact tgctcgactg
     1681 cgcgaagcgt gctctcgaag cactgtggcg cgatcgggaa cgcgacgggt tacttgtcat
     1741 cgtgcatcca tgggaagcag gtaccgacga ctcgccacgc ttcgacagct gggtcggctc
     1801 gagcacctgg agccgccggg tctggaccgc gttcgatcgc tcgctgctca agcgcacggt
     1861 attcagcccc gagggccagg ccatcgacaa tcctgggttc gtcgtcgcgc cggcgtcgtt
     1921 caacgccatc gcagcagacg ccgcggtaac gcttggcgaa ctcctcggcg atgcggactg
     1981 gatcgcgcgc ggccgagcac tcgccgatgc cctcgacgac gcagcgtggg acgccgatca
     2041 gtcgatgtgg cgcgacgtcg cgttcaccgg aggtggtgac agcgtgcaaa ttccgacgct
     2101 cgacgcgacg ttgcccgcga tgtgcacggc cgactctgcg aagtcatcgg cagcgttgc
//
LOCUS       DASWVF010000551         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_244310, whole genome shotgun sequence.
ACCESSION   DASWVF010000551 DASWVF010000000
VERSION     DASWVF010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244310"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1590
                     /locus_tag="VGD55_09740"
     CDS             223..1590
                     /locus_tag="VGD55_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY0870667.1"
                     /translation="MESSGFFPDPGVQQAPDTNTHESALVDDPLTSPNWSASEVPSFA
                     PSMLVQEPEVAAPPVESVFAPAASVAPLQPVAATLIERSSPDVVHARPVHESTTTIAG
                     LDLTALLASVVSLGASDLHLTAGARPMIRLNGSLQPLEDTPELSPPVIQTMLYAILTQ
                     RQREKFEAELELDFAYAVPGQARFRVNLYRQRDAVGAAFRLIPFDIKPLEALGIPPSV
                     SNFAMLPRGFVLVTGPTGSGKSTTLAGIVDLANRKRRDHIMTVEDPIEFLHQHKSCLI
                     NQREVGEDTHSFTNALKHVLRQDPDIILVGEMRDLETISVALTAAETGHLVFATLHTQ
                     DAAQTIDRVIDVFPPYQQQQVRVQLAGALQGVVSQTLCKTADGRGRAVATEVLVATPA
                     IRNLIREGKTHQIYSALQAGARHGMHTMDQHLAELVKGGRITYETALEKCNNVEDFNR
                     LSGRG"
     gene            1604..>2017
                     /locus_tag="VGD55_09745"
     CDS             1604..>2017
                     /locus_tag="VGD55_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0870668.1"
                     /translation="MTTTFQYTVRDRAGKLVKGTITAENQTIVMQRLKAGGYAPVSIT
                     QANSGLKKELTIPGFGGKKVKLKDLSVMARQFATMVNSGLSLLRTLSILSEQTTNSEL
                     ARVLGVVRNEVETGMALSAALAKHPRVFPPIMINMV"
BASE COUNT          390 a          679 c          599 g          349 t
ORIGIN      
        1 agggcatggt caccctgcgc cacgacggcc tggcgaaggt cgccgcggga gtcacgtcct
       61 tggaagagat cttccgcgtg gtcgtctgac gcaacgcaat ttggcgaacg gcgcgcaatc
      121 gtgcgccgcg gattcggccg accggctcaa ggagaacgag ctatcggtcg atgtgacgtc
      181 ctgaaccatg ccggacccgg caggtgcgaa aggggcttct cggtggaaag cagtggcttc
      241 ttcccggatc caggcgtgca gcaggcgccg gacacaaaca cacacgagtc cgccctggtg
      301 gacgatccgc tgacgagccc gaactggagc gcctctgagg tgccgagctt cgcgccgtcg
      361 atgctcgtgc aagagcccga ggtcgcggca cctccggtcg aatccgtctt tgccccagcc
      421 gcgtccgtcg cgccattaca gcccgtcgcg gccacgctca tcgaacgcag ctcgcccgac
      481 gtcgttcacg ccaggccggt gcacgagagc acgacgacga tcgctggcct cgacctgacg
      541 gcgctcctcg cgtcggtcgt ctcactcggc gcgtccgacc tgcacctcac agccggcgcc
      601 cgaccgatga ttcgtctcaa cggcagcttg cagccccttg aggacacccc cgaactgtcg
      661 cctccggtga tccagacgat gctgtacgcg atcctcaccc aacgccagcg cgagaagttc
      721 gaagctgagc ttgagctcga cttcgcctac gcagtccctg gtcaggcacg attccgagtc
      781 aacctttacc gccagcgcga cgcggttggt gcagcgtttc gtctgatccc gttcgacatc
      841 aagccactcg aagctctcgg catcccgccg tcggtctcga acttcgccat gctgccgcgt
      901 ggtttcgtcc tcgtcaccgg gcctacgggt tcgggcaagt cgacgacgtt ggccggcatt
      961 gtcgacctcg cgaaccgcaa gcgtcgcgat cacatcatga cggtcgaaga cccgatcgag
     1021 ttcttgcacc agcacaagtc ctgcctgatc aaccagcgcg aagtcggtga ggacactcac
     1081 tcgttcacca acgcactcaa gcacgtgctg cgccaggacc ccgacatcat cctggtcggt
     1141 gagatgcgcg acctcgagac gatctccgtc gccttgactg cagccgagac tggtcacctc
     1201 gtcttcgcca ccctgcacac ccaggatgcc gcccagacga tcgaccgtgt catcgacgtg
     1261 ttcccgcctt accaacagca gcaggttcgt gtgcagcttg ccggcgcgtt gcagggtgtc
     1321 gtcagtcaga cgctatgcaa gacagccgac ggtcgcggcc gcgcagttgc caccgaggtg
     1381 ctcgtcgcga ctcccgcgat tagaaacctg atccgcgaag gcaagacgca ccagatctat
     1441 tcggcactgc aagcaggcgc gcgtcacggc atgcacacga tggaccagca cctggcagag
     1501 cttgtcaagg gtggccgcat cacctacgag accgcgctcg aaaagtgcaa caacgtcgaa
     1561 gacttcaacc ggctttccgg ccggggatga cggggatcac gacatgacca cgacgttcca
     1621 gtacacggtc cgagatcgtg cgggcaagct cgtcaaggga accatcaccg ccgagaacca
     1681 gacgatcgtc atgcagcgcc tcaaggccgg cggctacgca ccggtcagca tcacgcaggc
     1741 caactccggg ttgaagaagg aactcacgat cccggggttc ggcggcaaga aggtgaagct
     1801 gaaggatctt tcggtcatgg cgcggcagtt cgccacgatg gtcaactcag gtctgtcgtt
     1861 gctgcgcact ctgagcatcc tttcggagca gacgaccaac agcgaacttg ctcgggtgct
     1921 cggcgttgtt cgcaacgagg tcgagaccgg tatggcattg tcagccgctc ttgccaagca
     1981 tccccgggtc ttcccaccga tcatgatcaa catggtg
//
LOCUS       DASWVF010000552         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_474101, whole genome shotgun sequence.
ACCESSION   DASWVF010000552 DASWVF010000000
VERSION     DASWVF010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474101"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VGD55_09750"
     CDS             <1..855
                     /locus_tag="VGD55_09750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0870669.1"
                     /translation="GPDSASLELVPPADASIATLWLTAYAVARAQNTKRKFYLVQDFE
                     PMFYPASTSYALAEETYRLGLYALCNTENLRQIYADDYGGKGMSFMPAIDPSVFHAHD
                     RPFRSDDTAATVFVYARPGHWRNCWELASLALRELKERLGDGVRIVTAGSWATDPDAA
                     DAIKQLGLLPYKATGELYRHCDVGLALTVSKHPSYLPLELMACGVPVVAFDNSWGHWI
                     LKDGENSLLARRTVPGLVDAMERMVVNAELRRTLATNAVADIAAHHGSWDAAFRGIHD
                     YLCDPENH"
     gene            861..>1703
                     /locus_tag="VGD55_09755"
     CDS             861..>1703
                     /locus_tag="VGD55_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0870670.1"
                     /translation="MEVVRRRIAVVTTEPLSAELAGPAIRAVELGRVLATEHDVEVAS
                     TASCSGPGPDANWQFVDAAALHALAARCDVIVVGGDVLAANTWLAEARPAIVVDLYDP
                     FHLEQLEQARDLGVAERRRIVFGTIDVLNTQAALGDTFLCASGRQRDMWLGHLASCGR
                     INPATYDADPTLRSLLRVVPFGVADSPPVAGAHALRGVVRGIGVDDEILLWGGGIYNW
                     FDPKTLILAVDKLRTDRPNLRLVFMGVQHPNPDVKTMRAAVEAWELSNKLELTDVHVF
                     FSDSS"
BASE COUNT          277 a          563 c          548 g          315 t
ORIGIN      
        1 gggcccgaca gtgcgagtct cgaactcgtg ccgccggccg atgcctcgat cgccacgctc
       61 tggctcacgg cgtacgccgt cgcgcgggcg cagaacacga agcggaagtt ctacctggtg
      121 caggacttcg agccgatgtt ctacccggcc agcacgtcgt acgcgctcgc agaggagacc
      181 taccggctcg gcctctatgc actgtgtaac accgagaacc tgcggcagat ctacgccgac
      241 gactacggcg gcaagggcat gagcttcatg cccgcgatcg acccttcggt gttccacgcg
      301 cacgaccggc cgttccgttc cgacgacacg gcagcaacgg tgttcgttta cgcgcgacct
      361 ggccactggc gcaactgctg ggagctcgcg tcgctcgcgc tgcgcgaact gaaagaacgt
      421 ctcggcgacg gtgtccgcat cgtcacggcc ggctcgtggg cgaccgatcc cgatgccgcc
      481 gacgcgatca aacaactcgg gttgctccca tacaaggcga ccggcgagct ctaccggcac
      541 tgcgatgtcg gcttggctct caccgtgtcg aaacacccgt cgtacttgcc gctcgagctg
      601 atggcgtgcg gagtgcccgt tgtcgcgttc gacaattcct gggggcactg gattctcaag
      661 gacggtgaga actcgctgct cgcccggcgt accgtgccgg gtctggtcga cgcgatggaa
      721 cgcatggtgg tgaacgccga gctgcgtcgg acgcttgcga ccaacgccgt tgccgacatc
      781 gctgctcatc acggcagttg ggacgccgcg tttcgcggca tccacgacta cctgtgcgac
      841 cccgagaatc actgacgccg gtggaggtcg tgcggcgccg gattgcggtc gtcacgaccg
      901 aaccgttgag tgcggaactc gccggtccgg cgattcgtgc cgtcgaactc ggtcgcgttc
      961 tcgccaccga gcatgatgtc gaggtcgcgt caaccgcgtc gtgcagcggg cccggccccg
     1021 acgcgaactg gcagttcgtt gacgccgccg cgttgcacgc gcttgccgct cggtgcgacg
     1081 tcatcgttgt cggtggcgac gtgttggcgg caaacacgtg gctggccgaa gcgcggccgg
     1141 ccatcgtggt cgacctctac gacccgttcc acctcgagca actcgagcag gcgcgcgatc
     1201 tgggcgttgc cgagcgtcgc cgcattgtct tcggcaccat cgatgtgctg aacacgcagg
     1261 cggcgctcgg cgacacgttc ctttgcgcgt caggacgcca gcgcgacatg tggctcggcc
     1321 atctcgcctc gtgcgggcgc atcaacccgg cgacctacga cgccgacccg acgcttcgct
     1381 cgctgcttcg agtggtgccg ttcggtgttg ccgactcacc accagttgca ggtgcccatg
     1441 cattgcgtgg tgtggtccga ggcattggcg tcgatgacga gatcttgctc tggggcggcg
     1501 gaatctacaa ctggttcgac ccgaagacgc tgatcctggc cgtggacaaa ctacgcactg
     1561 atcgaccaaa cctgcggttg gtgttcatgg gcgtgcagca cccgaaccca gacgtgaaga
     1621 cgatgcgagc cgccgttgag gcgtgggaac tctcgaacaa actcgagctg accgatgtcc
     1681 atgttttctt ctctgattcg tcc
//
LOCUS       DASWVF010000553         7361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_397465, whole genome shotgun sequence.
ACCESSION   DASWVF010000553 DASWVF010000000
VERSION     DASWVF010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397465"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGD55_09760"
     CDS             <1..257
                     /locus_tag="VGD55_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870671.1"
                     /translation="LHSLMRAQLGLTLGFIALAVGLLISLPLVVSLIPSLATRQVFGI
                     PLTLAVLGVAVYPVLVGIAIAYVRLAERTERHFLDLVEKL"
     gene            265..1986
                     /locus_tag="VGD55_09765"
     CDS             265..1986
                     /locus_tag="VGD55_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY0870672.1"
                     /translation="MTFAPLALAAIIVVTLITMVVGALGIRVARTPDDFMVAARAVPP
                     FRNAAAISGEYLSAASFLGVAGLILKDGLGMLWYPVGYAAGYLMLLLLVAAPLRRFGA
                     YTIPDFAEGRLGEPRLRQVATVFVLVIGWFYLLPQLKGAGLTLHVLLGTPYWVGVGVL
                     AVVVTSNIAAGGMKGVTFVQALQYWIKLVAIAVPAVVLLAVVGGLHRNALTKPAPPVF
                     SETTTVRVQTSVDINVVTPVTITAHGFIEGVRVDGPLTLNPGKYDVRDGAVLVFPAGS
                     PTPHASGLPILSGKSWVSPFVDAGHRGAHPLAATYSLIVATFLGTLGLPHILVRFYTN
                     RDGHSARRTTIIVLMLLGGFYIFPAVFAVLGRLRDPGLYLTGQTDSVVLVLPHALIGG
                     IGGSVLGALVAAGAFAAFLSTSSGLLVSVAGAMAHDLLRGGVRTFRRCAVVAGAVGLV
                     AGLQVQKFDLNMLVGWCFAIAASTFCPLLVLGIWWRRLTWVGAVAGLVLGGTTSSLAI
                     ILTMVGVGQRGWPGVLLGQPAIWTVPLAFFAMIAVSRATPAYLPADLESKLLQLHLPE
                     ALRPLRG"
     gene            complement(2019..2588)
                     /locus_tag="VGD55_09770"
     CDS             complement(2019..2588)
                     /locus_tag="VGD55_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870673.1"
                     /translation="MLQPGDLSADWSASGYVSDPNEAAESAALAKCVGGRDNYPDQTG
                     EAHSPDYSLGNATISSDAGSFKTPADIAADVAIIKSPKISTCYQQLAHAQLLASLPAG
                     STINTQSIVITAGTGGGPSNVVATGVGKFNVTVRGALNDLFINVAFITGPLTEAEIDF
                     SNVGSPVPASIQAPLIAKIAARAAAVAAV"
     gene            2596..2859
                     /locus_tag="VGD55_09775"
     CDS             2596..2859
                     /locus_tag="VGD55_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870674.1"
                     /translation="MSRAVAGGPIGALGDAAAGGKDAPGDEAPGDAPLEAGGLKSAPN
                     DAGAEGGVNVGVELFVHPVSTSEVHTTRTAHAEDFDNWDTSTD"
     gene            complement(2991..4280)
                     /locus_tag="VGD55_09780"
     CDS             complement(2991..4280)
                     /locus_tag="VGD55_09780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY0870675.1"
                     /translation="MTVFDDLVGQERVVALLRAAAVSAAEVVWAREMGLFESLEEAPA
                     VLPDAVAPAVPGAAMTHAWLFTGPPGSGRSVAARAFAAALQCTAGLQDGGVGCGVCAG
                     CHTVLAGTHADVEVVTPQGLSLGVREARELVARAALMPSGRHWQVILIEDADRLTEGA
                     ANVLLKAIEEPSPRTVWLLCVPSAQDLVITIRSRCRVVALRTPPTSAVASVLVSRDGV
                     APDVADFAARAAQGHVGRARRLATDADARSRRHAVLQLPSALTDVGRCLAVASELVES
                     VKADAAAAISLLDEAETTSYKEAFGEGGTATGVGRGSAGGAAARGALRSAAAALKELE
                     GRQKSRATRTQRDALDRALTDLAGFYRDVLVVQLGADVDIANVDEAVAISALAASSTA
                     EATVRRLSALLGCRTSLEANANPLLTVEALTLALRAG"
     gene            complement(4288..6363)
                     /gene="tmk"
                     /locus_tag="VGD55_09785"
     CDS             complement(4288..6363)
                     /gene="tmk"
                     /locus_tag="VGD55_09785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0870676.1"
                     /translation="MAVEVGGFRGVLAIKPFRKLWIALSLSSFGDWLGFLAQTALAAQ
                     LATGHTYLVQNSSVGLVVFVRLLPAVLLAPLAGAFADRLDRRLTMVIADIGRFSLYVS
                     IPIVHTLWWLFIATFLIETLSLFWIPAKEATVPNLVPRDRLEQANQLSLLTTYGSAPV
                     AALVFALLSLFTGVLGHAFHFFTTNRIDLALYFDGATFLVSAATIFTLREVSHQGGRR
                     RREASGAGDAPPISQPSVIRAIIEGWHFIGQTKVVRGLVIGMLGAFAAAGVVVGLAKR
                     YTLDLGGGDPAYGAMFGSVFVGLAVGMFLGPKFFRRLSRRRLFGVAITASGVTLAATA
                     VVHNLVLVVLGAAVMGAFSGVAWVTGYTLLGLEVEDEKRGRTWATLQSMMQVDLLLMV
                     AAGPFIAGAIGEHTLHIKRVVLRENGAAFTLLGAGIIALAVGVVAYRQMDDRDVPLWR
                     DLIGAIRGWHPLDGRGVETGLFIAFEGGEGAGKSTQSKLLATWLVERGYEVVTTREPG
                     ATPIGRQLRHLLLDPENGELAPRAEALLYAADRAQHVATVIRPALARGAVVVSDRYVD
                     SSLAYQGVGRALSTAEIDRLSQFATSGLRPDLTVLLDVRVSVGLARTVGRDAAPDRLE
                     AESTAFHERVRAAFRELAEAGADRYLVIDAGLSPEAIHQQIVRRISDMVPAMSPDLQP
                     TVQLKVGRR"
     gene            complement(6518..>7361)
                     /locus_tag="VGD55_09790"
     CDS             complement(6518..>7361)
                     /locus_tag="VGD55_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY0870677.1"
                     /translation="GTYLERDGQRANIPDGVAPDEVTVDYARALLDKPSGDRPLGVDP
                     VSGRELVAKDGRYGPYVTEILPEESKLKPRTASLFQSMSLDTVTIDDALRLLTLPRIL
                     GAVEGEEVVASNGRYGPYVKKGSETRSIESEEQLLTISLQQALDVLAQPKPRGRRAAA
                     AGPLRELGVDPANAKPIVVRDGRFGPYVTDGETNATLRKGDDVAAITLERAAELLADK
                     RARGPAPKKTAARRPAKKTATKSAAKSASKSVVKGAAKAASKGTAKKASPRKGAKKSA
                     TKRP"
BASE COUNT         1231 a         2498 c         2396 g         1236 t
ORIGIN      
        1 tgctgcactc acttatgcgt gcccagctcg gactcacctt gggattcatc gcactcgcag
       61 tcggactctt gatctcactc ccccttgtcg tcagcctcat cccgtcgctg gcgaccaggc
      121 aggtattcgg cattccgctc accctcgcgg tgctcggcgt tgcggtatat ccggtgctgg
      181 tcggcattgc gattgcctac gtccgtcttg ccgaacgaac tgaacgccac ttcttggatc
      241 tggtcgagaa gttgtgagca ataagtgacc ttcgcgccgc tcgctcttgc cgccatcatc
      301 gtcgtgactc tcatcacgat ggtcgtcgga gcgctcggta ttcgcgtcgc ccgcacaccc
      361 gacgacttca tggtcgccgc acgagccgtc ccgccctttc gcaatgccgc cgccatctcc
      421 ggcgaatacc tgtcggctgc atcatttctg ggcgtcgcgg gtttgattct caaagatggt
      481 ctcggcatgc tgtggtatcc ggtcggatac gccgccggat atctcatgct gctgctcctc
      541 gtcgcagcac ccttgcgacg attcggcgcg tacacgattc ccgacttcgc cgaaggccgc
      601 ctgggtgagc cacgtcttcg tcaggttgca acggtcttcg tgctcgtcat cggctggttc
      661 tacctattgc ctcaactgaa aggcgcaggc ctcacgctgc acgttctgct cggcactcct
      721 tattgggttg gcgttggcgt cctcgccgtt gtcgtgacga gcaatatcgc cgcgggcggc
      781 atgaagggcg ttaccttcgt gcaggctctc cagtactgga taaagctcgt cgcgatcgcg
      841 gtgccggccg ttgtgctgct cgcagtggtc ggcggcctgc atcgcaacgc gctcaccaag
      901 ccggcgccgc cggtgttctc ggaaacgaca actgttcgcg tgcagacctc ggtcgacatc
      961 aatgtcgtca cacccgtcac catcaccgcc cacggattca ttgaaggcgt cagggtcgac
     1021 ggcccgctca cgttgaatcc aggaaaatac gatgtgcggg acggcgcggt gctggtcttc
     1081 cccgcaggct cgccgacgcc gcacgcgtcg ggattgccaa tccttagcgg caaatcgtgg
     1141 gtatcaccgt ttgtcgacgc cggccatcgc ggcgcgcacc cgctggccgc gacctattca
     1201 ctgatcgtcg cgacatttct cggaacgttg gggctgccgc acatccttgt gcgcttctac
     1261 acgaatcgcg acggccattc tgcgcgtcgc acgacgatca ttgttctgat gttgctcggc
     1321 ggcttttata tcttccccgc tgtgttcgcc gtgctcgggc gattacgtga ccctggcctc
     1381 tatctcaccg ggcaaactga ttccgtcgtg ctcgtgctgc cgcatgcgtt gatcggcggc
     1441 atcggcggct cggtgctcgg cgcactggtc gcagcgggtg cgttcgccgc gttcctgtca
     1501 acgtcatccg gcttactcgt cagcgtcgca ggtgcgatgg cacacgatct gttgcgcgga
     1561 ggcgtgcgga cgttcaggcg atgtgcggtc gtcgcgggtg ctgtcgggtt ggtcgccggc
     1621 cttcaggtgc agaagttcga cctcaacatg ttggtcgggt ggtgctttgc gattgcggca
     1681 tcgacgtttt gcccgctgct ggtgctcgga atctggtggc gccgccttac ctgggtgggc
     1741 gcagtcgctg gcctggtgct cggcggtacc acgtcgtccc tggcgatcat tttgacgatg
     1801 gtgggtgtcg gacaacgcgg ctggcccggc gtcctgctcg gacagccggc gatctggaca
     1861 gtgcccctcg cgttcttcgc catgatcgcc gtctcgcggg cgacgcccgc ctacctgccc
     1921 gcggacctcg aatcgaagct gctccagctc cacctgccgg aggcattgcg gccgctgcgc
     1981 ggctaatgcg cgcgacaggt gatcgagcaa tgtcccgatc agacagccgc gacagccgct
     2041 gcccgcgcgg cgatctttgc aatcaacgga gcctgaatgc tcgcgggcac cggcgatccg
     2101 acgttcgaga agtcaatctc agcttcggtc agcggacccg tgatgaacgc gacgttgatg
     2161 aacaggtcgt tgagcgcgcc ccggacggtg acgttgaact tgccgacgcc ggtggcaacg
     2221 acattgctcg gtccgccgcc ggtgccagct gtgatgacaa tcgattgggt gttgatcgtc
     2281 gagccggccg gcagcgacgc aagcagctgg gcgtgggcca gctgctggta gcaggtcgag
     2341 atcttcgggc tcttgatgat ggccacgtcg gccgcaatgt cggccggcgt cttgaagctg
     2401 ccggcatcag atgagatcgt cgcgttcccg agggagtagt cgggtgagtg cgcttcaccg
     2461 gtctggtcgg ggtagttgtc gcggccgccg acacacttcg caagcgcggc gctctcggct
     2521 gcttcgttcg gatcggacac gtagccgctg gccgaccagt cggcggaaag gtcacccggt
     2581 tgcaacacga tcgctgtgag ccgcgcagtc gcaggtggtc cgatcggtgc gctcggtgat
     2641 gcagccgccg gcggcaagga cgcgcccggc gacgaggcac ccggcgacgc accgctggag
     2701 gcgggtgggc tgaagagtgc gccgaacgac gcaggcgctg agggcggcgt gaacgtgggc
     2761 gtcgaactct tcgtacaccc cgtcagcacg agcgaggtgc acacaaccag aaccgcgcac
     2821 gccgaagact tcgacaattg ggacacgtcc acagactagg tgctcggccg gtggcgggtg
     2881 cgagttgagc gaatcacggc gcgcttcggg acggtgctcg ccggcaacgt ggagcgccga
     2941 ggtcgtccta ggtgccgcca gactccacgt tgaccgggca gcggcgcgac ctagccggcg
     3001 cgtagggcca acgtcagcgc ttcgacagtc agcaacggat tcgcgttcgc ctcgagcgac
     3061 gtacgacacc cgagcagcgc cgagagccgg cgcaccgttg cttccgcagt cgacgatgcc
     3121 gccagcgcag aaatcgcgac cgcttcgtcg acgttcgcga tgtcgacgtc ggcgccgagt
     3181 tgcacgacga gcacgtcacg gtagaagccg gcaagatcgg taagcgcgcg gtcgagcgcg
     3241 tcgcgctgag tgcgcgtcgc acgtgatttc tgccgacctt ccagctcctt tagtgcagca
     3301 gccgccgatc gcagcgcgcc acgcgccgca gccccgccgg ctgacccgcg acccacaccg
     3361 gtcgccgtcc cgccctcgcc gaaggcttct ttgtacgacg ttgtctcggc ttcgtcgagc
     3421 agcgagatcg ccgctgcggc gtcggccttc acggactcca ccaactcaga agccacggcc
     3481 aggcagcgtc cgacatcggt caacgcagac ggcagctgca gaacggcatg gcggcgggag
     3541 cgcgcatcgg catcagtggc gaggcgacgc gcgcgcccga catggccctg cgccgcgcgc
     3601 gccgcaaagt cggcgacgtc aggagcaacg ccatctcgcg acaccagcac cgaggcgacc
     3661 gcactggtcg gtggcgtgcg caacgcgacc acgcgacacc gcgagcggat cgtgatcacg
     3721 aggtcttgtg ccgacggcac gcacaacaac cagactgtgc gcggtgaggg ctcctcgatc
     3781 gccttcaaca acacgttcgc tgcgccctcg gtcaagcggt cggcgtcctc gatcaagatg
     3841 acctgccagt ggcggcccga cggcatcaac gcagcgcgcg cgacgagttc gcgcgcctcg
     3901 cgcacgccaa gcgacaatcc ctgcggcgtg acgacctcga cgtcggcgtg cgttccggcc
     3961 agcacggtgt ggcacccggc gcaaacgccg caaccgacgc cgccgtcttg caacccggcc
     4021 gtgcactgca gcgcggccgc gaacgcgcgc gccgcgacgg accgccccga cccgggcgga
     4081 ccggtgaaca gccacgcatg cgtcatcgca gcacctggca ccgcaggagc aacagcgtcg
     4141 ggcagcaccg caggcgcttc ctcgagcgac tcgaacaggc ccatctcgcg ggcccacaca
     4201 acttcggccg ctgaaacggc agccgcgcgc agcaacgcaa caacgcgctc ctgcccgacc
     4261 aggtcgtcga agacggtcac cgtgaactca acgccgcccc accttcaact gcaccgtggg
     4321 ctgcaagtca ggtgacatcg ccggaaccat atccgagatc cgccgcacga tctgttgatg
     4381 aatggcctct ggggataacc ccgcgtcgat cacgaggtac cggtcagcgc cggcctcggc
     4441 cagttcgcgg aacgccgcac gcacccgctc gtgaaacgcg gtcgactctg cctccaaccg
     4501 gtcgggagcc gcatcgcgcc caaccgttcg agcaagcccg acgctgaccc ggacgtcgag
     4561 gagaaccgtc aagtcagggc gcagtcccga cgtggcgaat tgagacagcc gatcgatctc
     4621 ggccgtcgac aacgcgcggc caacgccctg gtaggcaagg gaggagtcga cgtagcgatc
     4681 gctgacgaca acagctccgc gcgcaagcgc cggcctgatc acggtcgcca catgctgagc
     4741 gcggtcggcc gcgtacagca gcgcctcggc tcggggggca agctcgccgt tctcggggtc
     4801 gagcaagagg tgccgcagtt ggcgcccgat cggcgtggcg ccaggctcgc gcgtggtgac
     4861 aacctcgtag ccgcgctcga cgagccaggt cgccagcagc ttgctctgcg tcgacttacc
     4921 ggcaccttcg ccgccctcga acgcgatgaa caggccggtc tcgacgccgc gaccgtcgag
     4981 cggatgccac ccgcgtatcg ccccgatgag gtcacgccag agcggcacat cacgatcgtc
     5041 catctgccgg taggccacca ccccgacagc caacgcaatg atgccggcgc cgagcagcgt
     5101 gaaggccgcg ccgttttcgc gcagcacgac ccgcttgatg tgcaaggtgt gctcaccaat
     5161 ggcacctgcg atgaacgggc cggcggcaac catgagaagc aggtcgacct gcatcatcga
     5221 ctggagcgtc gcccaggtgc ggccgcgctt ctcgtcttcg acttcgaggc cgagcaacgt
     5281 gtagccggtt acccaggcca cgccagagaa cgcgcccatg accgcggcac cgagaactac
     5341 gagcacgagg ttgtgcacga ctgcggtcgc ggccagcgtc acccctgacg cagtgatcgc
     5401 gacgccgaaa agccgacgac gggacaatcg ccggaagaac ttggggccga ggaacatgcc
     5461 gacggccaaa ccgacaaaca ccgaaccgaa catcgccccg tacgcggggt cgccgccgcc
     5521 gaggtcgagc gtgtaccgct tcgcgagacc gaccacgaca ccggccgccg cgaacgcgcc
     5581 gagcatgccg atcaccaggc cgcgcacgac tttggtctgg ccgatgaaat gccagccctc
     5641 gatgatcgcc cgaatgacgc tgggctggga gatcggcggg gcatcacccg cgccactggc
     5701 ttcgcgccgc cgccgtccgc cttggtggga gacctcacgc agggtgaaga tcgtcgccgc
     5761 ggacaccagg aacgtcgcgc cgtcgaagta cagcgcgagg tcgatccgat tcgtcgtgaa
     5821 gaaatggaac gcgtgaccga gcacgcccgt gaagagcgag agcagcgcga acacgagcgc
     5881 cgccaccggc gcggacccgt acgtcgtcag caggctcaac tggttggcct gctcgagccg
     5941 gtcgcgcggc accaggttgg gcacggtggc ttccttggcc ggaatccaga acaggctcaa
     6001 ggtctcgatg aggaaggtcg ctatgaagag ccaccacaag gtgtgcacga tcgggatcga
     6061 cacgtagagc gagaaccggc cgatgtcggc gatgaccatc gtgaggcgcc ggtcgagacg
     6121 atcggcgaaa gcgccggcga gcggagcgag cagcaccgcg ggcagcagtc gcacgaagac
     6181 aacaaggccg acggaggagt tctgcaccag gtaggtgtga ccggtcgcaa gttgcgcagc
     6241 cagtgccgtt tgggcgagga atccgagcca gtcaccgaag ctcgacagcg acagtgcgat
     6301 ccacagtttg cggaacggct tgatcgcgag cacgccccga aacccgccga cttcgacagc
     6361 cacccgggca gcgtcgacca gcggagccgc gttcggcccg gtgcccgcga ctggctcgga
     6421 aatcaggtac tcccgactgc cgcgaccgat gacgtactcg gcccttcacg gctcgctcgt
     6481 cccgctggat acctgcgagt atctcgcgtc ggtcgtttca gggtcgcttg gtcgcgctct
     6541 tcttggctcc cttgcgcggc gaagccttct tcgcggtgcc cttgctagcc gctttcgcgg
     6601 cccccttcac aacagatttc gacgcagact tcgcggcgct cttcgtcgcg gttttcttgg
     6661 cgggccgacg ggctgcggtc ttcttcggcg ccggcccgcg cgcccgcttg tcggcgagca
     6721 attcggccgc ccgctcgagc gtgatcgcgg cgacgtcgtc acccttgcgc agcgtggcgt
     6781 tggtctcgcc gtcggtcacg tagggtccga aacgaccatc gcggacgacg attggtttcg
     6841 catttgccgg atcgacaccg agctcacgca gcggaccggc ggccgccgct cggcgtccac
     6901 gcggcttggg ctgggcaagt acgtcgagcg cctgctgcaa ggagatagtc agcagctgct
     6961 cttccgattc gatcgaccgg gtctcggaac ccttcttgac gtagggcccg tagcgcccgt
     7021 tgctggcgac gacctcctcg ccctcgaccg cgccgagaat gcgcggcaat gtaagcagcc
     7081 gcaaggcgtc gtcgatggtc accgtgtcga gcgacatcga ctggaaaagg ctcgccgtcc
     7141 gcggcttgag cttcgactcc tcgggcaaga tctcggtgac gtacgggccg tagcgaccgt
     7201 ccttcgcgac cagttcgcgg cccgacaccg ggtcgacgcc aaggggccga tcgccgctgg
     7261 gcttgtcgag caaggcccgc gcgtaatcga ccgtcacctc gtcgggcgcg actccgtcgg
     7321 ggatgttcgc ccgctggccg tcgcgctcga ggtaggtgcc g
//
LOCUS       DASWVF010000554         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_40517, whole genome shotgun sequence.
ACCESSION   DASWVF010000554 DASWVF010000000
VERSION     DASWVF010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40517"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VGD55_09795"
     CDS             complement(<1..762)
                     /locus_tag="VGD55_09795"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEY0870678.1"
                     /translation="MPPSTLHRTAAAGGVAAALMVGESLSLAGTAGAVTPDAWAQLRA
                     CEASGNYAANTGNGFYGAYQFDARTWHGLGYSGVASQASPAEQDQAAQRLYQSRGWEP
                     WPSCSAKLGLVDDRTADRGTQRPPLAPVVVPTPTPAPSTAPAPIAVPAAPVVAVAAPA
                     PVAAPVVKAPVASPTSTVVAGVTGWHGQYLSVADVSHVRADVKAWQLEMVAAGYAITV
                     DGQYGPQSAAATTKFEAAHGLAVESPGIVGPQVWAA"
     gene            complement(906..1928)
                     /gene="ppk2"
                     /locus_tag="VGD55_09800"
     CDS             complement(906..1928)
                     /gene="ppk2"
                     /locus_tag="VGD55_09800"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY0870679.1"
                     /translation="MPSAPTQTPTSKRKRRNVMWESVDLTRSLDGLSLEADNEDLDDP
                     ILVGADGKRVNTWREHYPYEERLERGEYERQKRALQVELLKMQDWIHDVGARVCVLFE
                     GRDAAGKGGTIKRFMEHLDPRAARTVALGKPTEREAGQWYFQRYVGHLPTAGEMTLFD
                     RSWYNRAGVERVMGWTTDEKYKLFLEQAPVFERLLTDDGLILIKVWFSVSRSEQRTRF
                     IIRRIDPVRQWKLSPTDLALIDRWDDFTVAKAAMFDATDTDYAPWTVIRSNDKRRARV
                     AAMRHVLARVPYPDKDAGIIGKADSSIVGPPWKIYENDEQRIARPPGRTSSKTPEPTH
                     EAAAAH"
     gene            complement(2046..2990)
                     /locus_tag="VGD55_09805"
     CDS             complement(2046..2990)
                     /locus_tag="VGD55_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0870680.1"
                     /translation="MTSQQTRNARRQQRLVIAIALNVAVVVAQIIWGVLAHSLGLLAD
                     AGHNLADVAGLAIAVIALRYSLRSATMARSFGHHRATILAALANGGLLLAVTGFIAVD
                     AVVRLLHPKPVHGGIVALVALGAAVLNGVGAALVHERRGRYDLNMSAAKLHLVSDATV
                     SLAVAAAGLVIVLSHGTFWLDPAVSLVVCVAIASQAVRLLHRSADVLLESTPKAIDPE
                     AVLSAVSRVPTVVDVHDLHVWSLSSDIHAMSAHVAVSGHPTLEQAQATGEQIKEMVAT
                     EFDIAHATIEMECEACPEPDPCALESPSAFAVLHPHHH"
     gene            complement(3054..>3184)
                     /locus_tag="VGD55_09810"
     CDS             complement(3054..>3184)
                     /locus_tag="VGD55_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0870681.1"
                     /translation="MAWEVTDAEPAARAIVALLSDWFPKTTGEIVHVDGGVHAMGS"
BASE COUNT          530 a         1055 c         1071 g          528 t
ORIGIN      
        1 agcggcccag acctgtggtc cgacgatgcc ggggctctcg accgcaaggc cgtgcgcagc
       61 ctcgaacttc gtcgtggcgg cagccgattg cggaccgtac tgaccgtcga cggtgatcgc
      121 gtagccagcc gcaaccatct cgagctgcca ggccttcacg tcagcgcgaa cgtgtgagac
      181 atcggcgacg ctcaagtact ggccgtgcca gccggtcaca ccagccacga cggtgctcgt
      241 gggagacgcc accggtgcct tgacgacggg cgccgcgacg ggtgcggggg cggccacggc
      301 aacaaccgga gcagcgggga cggcgatcgg tgcaggcgcg gtcgaaggcg cgggtgtggg
      361 tgtaggcacg acgactggcg ccagcggtgg gcgctgagta ccgcggtcag ctgtcctgtc
      421 gtcgacgagt ccgagcttcg cactgcacga gggccacggc tcccaaccgc gcgactgata
      481 caggcgttgg gcagcctggt cttgctcggc tggtgacgcc tgagacgcga cgccggagta
      541 accgagaccg tgccaggtgc gggcatcgaa ctgatacgca ccgtagaagc cgttgccggt
      601 gttcgctgcg tagttgccgc tcgcttcgca tgcgcgcagc tgggcccagg catcgggcgt
      661 gacagctcct gcggtgcctg cgagtgagag cgactctccg accatcaacg ctgcggcgac
      721 tcccccggcg gcggccgttc gatggagggt gctgggaggc atcggccttg cgtgccgcgg
      781 gcccgagggt cgtccatgac gcggaccggc atagctgtgc aaacccatga cttccgactc
      841 gacacggacg gtccgtacct tgaccccgaa cagggcaaac gccacgtgca attcgcccgt
      901 tcggcctagt gcgccgcggc cgcctcgtgc gtgggctcgg gcgtcttcga cgacgtacgc
      961 ccaggcgggc gcgcgatccg ctgctcgtcg ttctcgtaga tcttccaggg gggtccgacg
     1021 atgctcgaat ccgccttgcc gatgatcccg gcgtccttgt cgggataggg aaccctggcc
     1081 agcacgtggc gcatggcagc gactcgggcc cgtctcttgt cgttgctgcg aatgacggtc
     1141 cacggcgcgt agtcggtgtc ggtggcatcg aacatcgcag ccttcgccac ggtgaaatcg
     1201 tcccagcggt cgataagcgc gaggtcggtc gggctcagct tccactgccg aactgggtcg
     1261 atgcgcctga tgatgaagcg ggtgcgttgc tcgctgcgtg acaccgaaaa ccacaccttg
     1321 atgaggatta acccatcatc ggtcagcaac cgctcgaaca ccggcgcctg ctcgaggaac
     1381 aacttgtact tctcgtccgt ggtccagccc atgacgcgtt cgacgccggc ccggttgtac
     1441 caggagcggt cgaacaaggt catttctccg gccgtcggca gatgacccac atagcgctgg
     1501 aagtaccact gcccagcctc gcgctcagtg ggcttgccca gggccacggt gcgggctgcg
     1561 cgcggatcga ggtgctccat gaaccgcttg atcgtgccgc ctttgccagc agcatcgcgg
     1621 ccctcgaata gcacgcacac ccgcgccccg acgtcgtgaa tccagtcctg cattttcagc
     1681 aactcgactt ggagtgcacg cttctggcgt tcgtattcgc cacgctcaag ccgttcctcg
     1741 taggggtagt gctcccgcca ggtgttcacg cgcttgccgt cggccccgac gaggatcggg
     1801 tcgtccaagt cctcgttgtc tgcctcgagg ctcagaccgt ccagagaacg cgtgagatca
     1861 acgctctccc acatgacatt gcggcgcttc cgcttcgatg tcggggtttg ggtcggcgct
     1921 gacggcatgc gttactccct ttccgttcgc tcgccgagaa tctgccgcat ctgggacgcg
     1981 gacagcaaga tcgcgggtgt cgcgagggtg aacggcgggt gaagtctcgc ggcaccgcat
     2041 cgcacctagt ggtggtgcgg gtgcagcacc gcgaacgccg agggtgattc gagggcgcac
     2101 ggatcgggct cggggcacgc ctcacattcc atctcgatcg tggcgtgcgc gatgtcgaac
     2161 tcggtcgcca ccatctcctt gatctgctca ccggtcgcct gcgcctgctc gagcgtgggg
     2221 tggccggaga cagccacgtg agccgacatc gcatggatgt cactcgaaag actccatacg
     2281 tgcaagtcat ggacgtcgac cacggtcggc acgcggctca ctgcggagag cacggcctcg
     2341 gggtcgatgg ccttgggcgt cgattcgagc agcacgtcgg cgcttcgatg caggagccgc
     2401 actgcctggc tcgcgatggc gacgcacacc actaacgaca cggcggggtc gagccagaac
     2461 gtgccgtggc tcagcacgat caccagcccc gccgctgcga cggccaacga caccgttgcg
     2521 tcggagacga ggtgcaactt cgcagccgac atgttcaggt cgtagcggcc gcggcgttcg
     2581 tgcacgaggg cggcaccgac gccgttgagt accgcggcgc cgagcgccac cagcgcaacg
     2641 atgccgccgt gaaccggctt cggatgcagt agccggacga cggcgtcgac cgctatgaag
     2701 ccggtgaccg cgagcagcaa cccgccgttg gccagagccg ccaggatcgt cgcccgatgg
     2761 tgaccgaacg agcgtgccat ggtggcactt cgcagcgaat accgcagcgc gatcaccgcg
     2821 atcgccagcc ccgcgacgtc cgccagattg tgccctgcgt cggcaagaag cccgagtgaa
     2881 tgagcgagca ctccccagat gatctgggcg acgacgaccg ccacgttgag cgcaatcgcg
     2941 atgaccagcc gctgctggcg gcgcgcgttg cgggtctgct gggacgtcac ctgctcaacg
     3001 gtacggctcg tggggccgat gggcgccaag acgtgcatca aaacggacgc cgatcagctg
     3061 cccatcgcgt ggacgccgcc gtcgacatgg acgatctcgc cggtcgtctt ggggaaccag
     3121 tcagacagca gcgcgacgat tgcccgggcc gcgggctcgg catcggtgac ctcccacgcc
     3181 atcg
//
LOCUS       DASWVF010000555         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_65700, whole genome shotgun sequence.
ACCESSION   DASWVF010000555 DASWVF010000000
VERSION     DASWVF010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65700"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGD55_09815"
     CDS             complement(<1..347)
                     /locus_tag="VGD55_09815"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY0870682.1"
                     /translation="MSEAGPTKTRGSNGTPAPPVDLVTTPSPPRHEEDELLEHQDTFY
                     IQATSSRTDDRTRVLKNSDTFGVFDRFGDVQPVGLGEQGVYDDGTRFLSRLELRIGGR
                     RPLLLSSTVRKEN"
     gene            complement(344..1423)
                     /locus_tag="VGD55_09820"
     CDS             complement(344..1423)
                     /locus_tag="VGD55_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0870683.1"
                     /translation="MRIAQVSPLYESVPPKLYGGTERVVYFLTEALLDLGHEVTLFAS
                     GDSRTRARLIPTVPIALRLDANCRELVAPHVQLIEEVMRRAAEFDVIHFHVDYFGFPL
                     ASRSNVPHVTTQHGRLDIPELEPLFRLFPNEPQVSISDAQRGPLAFANWAATVYHGLP
                     PDIYRLQPERGSYLAFLGRISPEKGVDHAIEIAQRLDMPLKIAAKIGKPEQSYYEQEI
                     KPLLADPRIEFVGEIGEAEKQDFLGNAAALLFPVEWPEPFGLVMIEAMACGTPVIAFR
                     RGAIPEVVQDGVSGFVVDSVEEAVAATPRAIALPRAQCRAGFEARFLVERMARDYMAV
                     FESLCEVERGSRWVRARRAAGAEIT"
     gene            complement(1513..>1732)
                     /locus_tag="VGD55_09825"
     CDS             complement(1513..>1732)
                     /locus_tag="VGD55_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870684.1"
                     /translation="DNSRLLQAWEYCGKRSRKGKKVQDMEDFLVAFGDYMEARNDVAK
                     TAREEAARMLGQPLPKEKEKKEGASAAP"
BASE COUNT          272 a          599 c          564 g          297 t
ORIGIN      
        1 tcgttctctt ttcggacggt cgagctgagc agcagcgggc gacggccccc aatccgcagc
       61 tccagtcggg acagaaaccg cgtgccgtcg tcgtacaccc cctgttcgcc gagcccgacc
      121 ggctgcacgt cgccgaagcg atcgaacacc ccgaacgtat cgctgttctt gagcacgcgc
      181 gtgcggtcgt cggttcgcga cgaagtcgcc tggatgtaga aagtgtcttg gtgctcgagg
      241 agctcgtcct cctcgtgacg cgggggactg ggcgtcgtca ccagatcgac cggcggcgcg
      301 ggcgtcccgt tcgagccgcg cgtcttcgtc gggcctgctt cgctcacgtg atctccgcgc
      361 cggcggcccg ccgcgcgcgc acccaccggc tgcccctctc aacctcgcag aggctctcga
      421 agaccgccat gtagtcgcgg gccatccgtt cgaccaggaa gcgcgcctcg aaaccagcgc
      481 ggcactgggc gcgcggaagc gcgattgccc gcggcgtggc ggcgaccgct tcctcgacgg
      541 agtcgacgac gaatccggac acgccgtcct ggacgacttc cggaatcgct ccgcgtcgaa
      601 acgcaatcac gggcgtgcca caggccatcg cctcgatcat gaccagtccg aagggctcgg
      661 gccactcgac gggaaagagc agcgccgccg cgtttcccag gaaatcttgc ttctccgctt
      721 caccgatctc gccgacgaac tcgatgcgcg ggtccgcgag cagcggtttg atttcctgct
      781 cgtagtagga ctgctcgggc ttgccgatct tggcggcgat cttcagcggc atgtccagcc
      841 gctgggcgat ctcgatggcg tggtcgactc ccttttccgg cgagatgcgc cccaggaacg
      901 ccagatagct gccgcgctcg ggctgaaggc ggtaaatgtc gggcggcagg ccgtgataca
      961 cggtcgcggc ccagttcgcg aaggcaagcg gcccacgctg cgcatccgag atcgacacct
     1021 gcggctcatt ggggaacagc cggaacagcg gctccagctc ggggatgtcg aggcgcccgt
     1081 gctgagtcgt cacgtgcggc acgttggacc gcgacgccag cgggaacccg aagtagtcca
     1141 cgtggaagtg gatgacgtcg aactcggccg cccgtcgcat gacctcttcg atgagctgca
     1201 cgtgcggcgc gacgagctcg cgacagttgg cgtccagacg cagggctatc ggcaccgtcg
     1261 ggatcagccg tgcgcgtgtc cgcgagtcgc cgctcgcgaa caaggtcact tcatgaccga
     1321 ggtccagcaa ggcctcggtc aggaagtaga cgacccgctc ggtcccgccg tacagcttgg
     1381 gcgggacgct ctcgtaaagt ggtgacacct gcgcgatccg catcccgccc tcccggctcg
     1441 ggaaataacg taatcacgca ggggcgcctt ggccggcgcc ccggaacgct cgaggctggg
     1501 gcgcagcgcg tttcagggcg cggccgacgc gccttctttc ttttccttct ccttgggcaa
     1561 cggctggccc agcatgcggg cggcctcttc gcgcgccgtc ttggcgacgt cgttacgggc
     1621 ctccatgtag tcgccgaacg cgaccaggaa gtcctccatg tcctgcacct tcttgccctt
     1681 gcgcgaccgc ttgccgcagt actcccacgc ctgaagcaac cggctgttgt ct
//
LOCUS       DASWVF010000556         5852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_397482, whole genome shotgun sequence.
ACCESSION   DASWVF010000556 DASWVF010000000
VERSION     DASWVF010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397482"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1574
                     /locus_tag="VGD55_09830"
     CDS             213..1574
                     /locus_tag="VGD55_09830"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY0870685.1"
                     /translation="MKGSRSFPADFVWGVATSAYQIEGAHDEDGRRPSIWDTFCRQPG
                     AIDNADTGDVACDHYHRWQEDLDLVEQLGADAYRFSVAWPRVIPGGTGAVNQKGLSFY
                     DALVDALLAKGITPFVTLYHWDLPQDQQDKGGWPLRDTAYAYADYASVVVTALGDRVA
                     NWVTVNEPLCSAWLGHLEGVMAPGVKDIAAAVPAAFHLHLAHGLGVQAIRSAAKLTPS
                     VGIVNNLSPVVAATDRAEDVAAAQRGDGHTNRWWLDPIHGRGYPHDMVEVYGVEPPVQ
                     GNDLDVISQPLDFLGINYYFREVARDDPQGVTPRVAHVRVPDALETAMGWEVYPQGLE
                     DILVRVADEYAPMKIFVTENGSAWADHPDADGDVDDVERAQYLESHIEACAGALSRGA
                     PIGGYFAWSLLDNFEWAYGYDKRFGLLRVDYDTQRRTIKTSGRRFTDIVERHRASIPE
                     VVA"
     gene            1593..2567
                     /locus_tag="VGD55_09835"
     CDS             1593..2567
                     /locus_tag="VGD55_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870686.1"
                     /translation="MRIFVRRIGFYLLAAVAAITLDFFIPRMVPGDPVLTELGTMKNA
                     PPQALAALELQYGGTHQSMLHQYLHFWNMLLHGDLGYSLSLGNVPVTHIIAQHMYWTV
                     VLVGLSTIISFVVGTTIGTLAAWKRGSWLESLLPITTFLQAMPYFFMATLLVLLFSVH
                     FKFFPSVGGYDFTHSSIGLNWKFIKDAAYHGILPALTIVGASLAGWIIGMRNMVVTVR
                     DEDYVLLAEAAGLPKHRVIWYAVRNAILPQVSSFALALSFVVGGSLLTEIVFSYPGIG
                     FTLLAAVKSLDYSLVQGLFLVITLVVLAANFVADIVYVALDPRTRQEA"
     gene            2574..3650
                     /locus_tag="VGD55_09840"
     CDS             2574..3650
                     /locus_tag="VGD55_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870687.1"
                     /translation="MAIVEVSAGNAAGSVPSSAARRPRRQFRLGRSKKMLVGLIILGC
                     FLVVAVIGPIVAPYHPNAIGPLVGPPRHGSTVPTQLPGAHHLLGQTNIGGDVFSQLLA
                     GTRPTILVSFLAGFIATFLAIVIGITAGYIGGLVDEVLSVIANVFLVIPALPLLIAVG
                     AFLGPTRTSDPLIIGVIIALTGWAWGARVLRAQAMSMRSRDFVEAARVSGESAWRIIM
                     TEIMPNLTPVIASSFLFTTLYAIGTYVGLGFLTVVGAGVDYNWGTMLFDATSSSAVES
                     GFWWWYIPPGIAIAVLGTSLALINFGIDEFINPRLRIATGGRKVRKSLGFKLRPQLGF
                     TPVIRTQAIDASRGVRGAGLEEAL"
     gene            3647..4879
                     /locus_tag="VGD55_09845"
     CDS             3647..4879
                     /locus_tag="VGD55_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870688.1"
                     /translation="MTLTNGANGRSPASSSSQASQRPTHHGADLLLEVRGLSVDYGLG
                     DHPVHAVDDVSLSIRRGEVVGLAGESGSGKSTLAYAVTRLLRDPGVITGGEVSFYDWP
                     KGSYRASGAGDFDDTVGPGHATATTEPRIVDLLSDDRDVVRAVRWSDIAVVFQSALHA
                     LNPVLRIGRLIDDVLKTHEKDMTRAQRQARAKELLEMVGISADRLSSYPHELSGGMRQ
                     RVMIAVAVALRPSLLIMDEPTTALDVVIQREILEGLMQLREQLGFSVLFITHDLSLLI
                     EIADTIAVMYAGRLVEKAPAKALFRAPRHPYTYGLTKSFPSLHGEKKVMAGIEGSPPD
                     LRKIPSGCPFHPRCAWAMDICATQDPPVLTLHDASGSREVLCWLQDGAHEVPPELNRV
                     PPAAGSAAALPSMRGRRP"
     gene            4876..>5852
                     /locus_tag="VGD55_09850"
     CDS             4876..>5852
                     /locus_tag="VGD55_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870689.1"
                     /translation="MIEHDDVPLLEAVDVTKHYRVRVGRAGRSVVHAAENISVALYPG
                     TVTAVVGESGSGKSTLSRMLARTQKLTSGKLYFQGEEVSLSKRVPADYRRSVQLVLQD
                     PFESLNPVHTVRYILSRPLIIHGRTGGNTEQAVLNLLRRVALEPAEQFIGKYPHELSG
                     GQRQRVSIARGLAVHPKVLLADEPVSMLDVSIRLGVLNLLADLRDRERLAILYVTHDI
                     ASARYLADTIMVMYAGKVVEVGPADQITNFPAHPYTKLLLSAAPDPDADEKVWPGAAG
                     APPSLVQPPSGCRFHPRCAYAMPICSTDLPPAFRVAETDDAGPHVADNHT"
BASE COUNT          994 a         1867 c         1843 g         1148 t
ORIGIN      
        1 ttacgaccgt gcggcagcca ttgcgggaga tgggagatgc agccgctcgc atgctgctct
       61 cgtatttcga gggttcacca gtttctgaga cgcgacaggt gatcccgacg acgctcgtcg
      121 tgcggagttc ctccatacca gcgtgagccg cacaagacgg gtgcccgaac agagttgtgc
      181 ctgaacacag aagcgatgga gggacattct tcgtgaaggg ttcgagatct ttccccgccg
      241 acttcgtgtg gggggtggcg acgtccgcgt atcagatcga aggcgctcac gacgaagatg
      301 gacgccgacc atcgatctgg gacacgtttt gtcgccaacc cggcgcgatc gacaacgccg
      361 acaccggcga cgtcgcgtgc gaccactacc accggtggca ggaagatctc gacctcgtcg
      421 agcagttggg cgccgacgct tatcgcttct ccgtcgcatg gccgcgggtg atcccgggcg
      481 gtaccggcgc cgtgaatcag aagggcctca gcttctacga cgcgctcgtc gacgccctgc
      541 tggcgaaggg catcacgccg ttcgtcacgc tctaccactg ggatctgccg caggaccagc
      601 aagacaaggg cggctggccc ctgcgcgata cggcatacgc gtacgccgac tatgcctcgg
      661 tggttgtgac cgcgcttggt gatcgcgtcg ccaattgggt gactgtgaac gagccgctgt
      721 gttcggcgtg gcttggtcac cttgaaggcg tgatggcccc gggcgtcaaa gacatcgctg
      781 ccgcggtgcc cgctgcgttt catctgcacc tcgctcacgg gctcggcgtc caggcgattc
      841 ggtctgccgc gaagctcacg ccgtcggttg gcatcgtgaa taacctcagc ccggtggtgg
      901 ccgcgacaga tcgcgcagaa gacgtcgcgg cggcacagcg cggcgacggg cacaccaaca
      961 gatggtggct ggatccgatc cacggccgtg gatatccgca cgacatggtc gaggtttacg
     1021 gagtcgagcc tcccgttcag gggaacgacc tcgacgtgat ctcccaaccg ctcgacttcc
     1081 tcggcatcaa ctactacttc cgcgaggtgg cgcgagacga cccacagggc gtgacacctc
     1141 gagtggcgca tgtccgcgtg cccgatgcgc tggaaaccgc gatgggctgg gaggtctacc
     1201 cacaggggct cgaggacatc ttggtccgtg tcgccgacga gtacgcgccg atgaaaatct
     1261 tcgtcaccga aaacggctct gcctgggccg atcaccccga cgccgacgga gacgtcgatg
     1321 acgtcgagcg tgctcaatac ctcgaatccc acatcgaggc gtgcgccggc gcgttgagcc
     1381 gtggggcccc cattggcggc tacttcgcgt ggtcccttct cgacaacttc gagtgggcat
     1441 acggctacga caagcgcttc ggattgttgc gggtcgacta cgacactcaa cgacgcacca
     1501 tcaagacgag cggccggcga ttcactgaca tcgtcgagcg gcaccgcgcg tcgatccctg
     1561 aggtcgtagc gtgaacgggg cggggtgaag atatgcgtat atttgtgagg cgtatcggct
     1621 tctatctgct ggcggccgtc gctgcgatca cgctcgactt cttcatcccg cgtatggtgc
     1681 cgggcgatcc ggtgctcacc gaactcggca ccatgaagaa cgcgccgcca caggcgctcg
     1741 ccgctctcga gctccagtac ggcggtacgc accaaagcat gcttcatcag tacctgcact
     1801 tttggaacat gctgcttcac ggcgatctcg gctactcgtt gagcctgggt aacgtgcccg
     1861 tcactcacat catcgcccag cacatgtact ggacagtcgt gctcgtcggt ttgtcgacga
     1921 tcatcagctt cgtcgtcggt acgacaatcg gcacgctcgc ggcctggaaa cgtgggtcgt
     1981 ggctcgaatc gttgcttccg atcacgacgt tcctgcaggc catgccctac ttcttcatgg
     2041 ctacgttgct cgtgctgctt ttctcggttc acttcaagtt cttcccatcg gtgggcggtt
     2101 atgacttcac ccacagctcg atcgggctga actggaagtt catcaaggac gccgcctacc
     2161 acgggatcct gcctgcactg acgatcgtcg gcgcgtcgct cgccggctgg atcatcggca
     2221 tgcgcaacat ggtggtgact gtgcgcgatg aggactacgt gttgcttgcc gaggccgccg
     2281 ggttaccaaa gcaccgggtg atttggtacg cggttcgaaa cgcgatcctt ccccaggtct
     2341 cgagtttcgc gttggcgctc agcttcgtgg tcggtggctc gctcctgacc gaaatcgtct
     2401 tctcgtatcc cggtatcggc ttcaccttgc ttgcagcggt caagagcctc gactactccc
     2461 tggtgcaggg cctcttcctg gtcatcacgc tggtcgtcct cgctgcgaac ttcgtggcag
     2521 acatcgtgta cgtcgcgctc gatccacgca cccgacagga ggcgtgagcg acggtggcga
     2581 ttgtcgaggt ttcagcaggc aatgcagcgg gatcggtccc atcgtctgca gcgcggcgcc
     2641 ctaggaggca attcaggctc ggccggtcga agaagatgct cgtgggattg atcattctcg
     2701 ggtgcttcct ggtcgtcgcg gtcatcggtc cgatcgtcgc gccctaccac ccgaacgcga
     2761 tcggccctct tgtcgggccg ccgagacacg gctcgacggt cccaacgcaa ctccccggcg
     2821 cgcaccatct gctaggtcaa accaacatcg gtggcgacgt tttctcccag ctccttgctg
     2881 gcacgcggcc gacaatcctg gtgtcgttcc tggccggttt catcgcgacg ttcctcgcca
     2941 tcgtgatcgg gatcaccgcc gggtacatcg gcggtctggt cgacgaggta ctgtcggtga
     3001 tcgcgaacgt gttcctggtc atccccgcac tgcctttgct catcgctgtc ggcgcgttcc
     3061 tcgggcctac ccgcaccagc gatccgctga tcatcggtgt catcatcgcc ttgaccgggt
     3121 gggcctgggg ggcacgggtg ctccgcgccc aagcgatgtc gatgcggagc cgcgacttcg
     3181 tcgaggcggc tcgagtcagc ggtgagagcg cgtggcgcat catcatgacc gagatcatgc
     3241 cgaacctgac gccggtcatc gcgtcgtcgt ttctcttcac cacgctctac gccatcggaa
     3301 cgtacgtggg tctgggattt ctcaccgttg tcggggctgg tgtcgattac aactggggca
     3361 cgatgctgtt cgacgcaaca tcgagctcag cagtcgagtc tggcttctgg tggtggtata
     3421 tccctccggg aattgccatc gcggttctcg gcacgagtct ggcgttgatc aacttcggta
     3481 tcgacgagtt catcaatccg cgactgcgga ttgccaccgg cgggcgcaag gttcgcaaga
     3541 gccttggctt caagttgcga ccgcagttgg gcttcacccc cgtcatccgc acgcaggcga
     3601 tcgacgcgag ccgtggggtg cggggcgccg gcctcgagga ggcgctgtga ccctgacaaa
     3661 cggcgcgaac ggacgatcgc ccgcctcgtc gtcatcgcag gcttcgcagc gaccgacaca
     3721 ccacggtgcc gatttgttgt tggaggtccg cggattgagc gtcgactacg ggttgggaga
     3781 ccatcccgtg cacgctgtcg acgacgtcag tctttcgatt cggcgcggcg aggtggtcgg
     3841 tctcgccggg gagagcggca gtgggaagtc aaccctcgcc tacgccgtga ccaggctcct
     3901 gcgagaccca ggggtcatca ccggcggtga ggtttctttc tatgactggc cgaagggttc
     3961 gtatcgggcc tctggcgctg gcgatttcga tgacaccgtc ggtccggggc atgcgacagc
     4021 cacgaccgag ccacgaatcg tcgacttgct gagcgatgac cgcgacgtcg tgcgtgcggt
     4081 tcgctggtcc gacattgctg ttgtgtttca aagtgccctg cacgctctca atccggtcct
     4141 acggatcggc cgtctgatcg acgacgtgct gaagacgcac gagaaagaca tgacgcgggc
     4201 gcagcgccag gctcgcgcga aggaactcct cgagatggtg ggcataagtg ccgaccggtt
     4261 gagcagctac ccgcacgagt tgtccggcgg tatgcgacag cgcgtcatga tcgccgtcgc
     4321 cgtagcgctg cggcccagcc tcctgatcat ggacgaacct acaacggcgt tggacgtcgt
     4381 catccaacgc gagattctcg aagggttgat gcagctgcgc gaacagctcg ggttcagcgt
     4441 gctgttcatc acccatgacc tgtcattgct gatcgagatc gccgacacga tcgcggtgat
     4501 gtacgcgggc cggttggtcg aaaaggcacc tgcgaaggcg ctgttccgtg cgccccgcca
     4561 cccctacacc tacgggctca caaagtcatt cccgtcgttg cacggcgaga agaaggtgat
     4621 ggccggcatc gagggctcac cgcccgacct acggaagatt ccgagtggct gcccgttcca
     4681 ccctcgctgc gcctgggcga tggatatatg cgcgacccaa gacccgccgg tgctcacgct
     4741 gcacgatgca tcgggcagtc gcgaggtctt gtgttggctg caagacggcg cccacgaggt
     4801 tccccccgaa ctcaatcggg ttccaccggc cgcaggctcc gccgccgctc ttccgagtat
     4861 gcgcgggcgg cggccgtgat cgagcacgac gacgtgccgt tgctggaagc ggtcgacgtc
     4921 acgaagcact accgggtccg ggtcgggcgc gcgggtcgaa gcgtcgtgca tgcggcagaa
     4981 aacatttcgg tcgcgctgta tccgggcacc gttacggcgg tcgtgggcga gagcggatct
     5041 ggcaagtcga cgctgtcgag aatgcttgcg cgcacccaga aactcacctc gggcaagctg
     5101 tacttccagg gcgaagaggt aagcctgtcc aagcgggtac ctgccgacta tcggcgttct
     5161 gtgcagcttg tgttgcagga tccgttcgaa tcgctcaacc ctgtccacac ggtgcgctac
     5221 atcttgagcc gacccctgat catccacgga cgcactggcg gcaacaccga gcaagccgtc
     5281 ttgaacctcc tccggcgggt tgcgctcgag cccgccgagc agttcatcgg caagtacccg
     5341 cacgagcttt ccggcggcca acgccagcgg gtatcgatcg cacgaggatt ggctgtgcac
     5401 cccaaggtgc tgctggccga cgagccggtg tcgatgctcg acgtatcaat ccgactcggt
     5461 gtcttgaacc tgctcgctga ccttcgtgat cgagagcggc tggcgatcct gtacgtgacg
     5521 cacgacatcg cgtctgctcg ctatctcgcc gacacgatca tggtgatgta cgccggcaag
     5581 gtggtcgagg tcgggcctgc agaccagatc accaacttcc cggcccatcc gtatacgaag
     5641 ttgctgctga gcgcggcgcc ggatcccgac gccgacgaga aggtgtggcc cggggctgcg
     5701 ggtgcgccac cctccctcgt gcaaccgccg tccggctgca ggtttcaccc tcgctgcgcg
     5761 tatgccatgc cgatttgctc aaccgaccta ccgcccgcgt ttcgggtcgc cgaaaccgac
     5821 gatgccggcc cgcacgttgc cgacaaccac ac
//
LOCUS       DASWVF010000557         9353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_40528, whole genome shotgun sequence.
ACCESSION   DASWVF010000557 DASWVF010000000
VERSION     DASWVF010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40528"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1413)
                     /gene="thrB"
                     /locus_tag="VGD55_09855"
     CDS             complement(466..1413)
                     /gene="thrB"
                     /locus_tag="VGD55_09855"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0870690.1"
                     /translation="MGVSFRAAPARVRVPATSANLGPGFDSFGLALALYDDVVVRVAE
                     SGLVVDVAGEGADDVRRDKRNLVVQAVRATFDALGGQPRGLEIVCANRIPHGRGLGSS
                     AAAIVAGVVAARELVLGGAERLPDDAVLRLAAQLEGHPDNVAACLAGGFTMAWTEPGT
                     EPGTTGSPALLTRALHLDVHKSIAAVAFVPDTKSSTTKARKLLPESVPHHEAAANAAR
                     AALLAQALTRRPELLLVATQDALHQQYRASAMPRSAALLARLREAGIAAVISGAGPTV
                     LALTTTAQAETVAAFDARWFTASQIAIDTQGARVMPLAG"
     gene            complement(1413..2480)
                     /gene="thrC"
                     /locus_tag="VGD55_09860"
     CDS             complement(1413..2480)
                     /gene="thrC"
                     /locus_tag="VGD55_09860"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0870691.1"
                     /translation="MSARPRWRGVIEEYREWLPVTDATPVVTLQEGGTPLLPAPVLSG
                     LTGCDVFLKVEGANPTGSFKDRGMTMAISKAVEDGSKAVICASTGNTSASAAAYAARA
                     GLLCAVLVPRGKIALGKLAQALVHGAKLLQVDGNFDDCLSLASKLAQDYPVTLVNSVN
                     PDRIQGQKTAAFEIVDALGDAPDVHCLPVGNAGNITAYWKGYVEYAAAGRAANRPKMR
                     GFQAAGSAPIVLGHVVENPSTIATAIRIGNPASWVNALDARDQSGGAIESVTDRQILS
                     AYRLLASKEAVFVEPASAASVAGLLMAHEQGTLPAGLRVVCTVTGHGLKDPEWAISGA
                     PAPITIAADAMLAARHLGLDA"
     gene            complement(2477..3781)
                     /locus_tag="VGD55_09865"
     CDS             complement(2477..3781)
                     /locus_tag="VGD55_09865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY0870692.1"
                     /translation="MSGRPLRIALLGCGVVGVEVARLLTEQADDLAARVGVPLELAGV
                     AVRRLNRKRDIDLDPALFTTDAAALVARDDIDIVVEVIGGIEPARSLLLTAMSHGASV
                     VTANKALLAEDGATLYDAASKHGVDLYFEASVAGAIPLLRPLRESLAGDTVHRVLGIV
                     NGTTNFILTRMDETGAGFAEALDEATALGYAEADPSADIDAFDAAAKAAILAGLAFHT
                     RVTIADVHREGIGDVTATAVLSARDMGCVVKLLAICERTADGRGVSVRVHPAMIPRSH
                     PLAGVREAYNAVFVEAEAAGQLMFYGRGAGGAPTASAVLGDIVAVARNKVKGALGPGE
                     SAYADLELRSMGETLTRYHISLDVADKAGVLAQVANVFSDHDVSIQAVRQSGRGDDAS
                     LVIVTHTASDAALSATVADLRTLDIVRDVASVMRVEGEDTDR"
     gene            complement(3917..5314)
                     /gene="lysA"
                     /locus_tag="VGD55_09870"
     CDS             complement(3917..5314)
                     /gene="lysA"
                     /locus_tag="VGD55_09870"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY0870693.1"
                     /translation="MHTHEAGALHADAREPLSPTPVAPADVNALDPTIWSSNVTRSDA
                     GVLTIAGVAATDLARDFGTPAYVVDEADFRARCQNWVGAFPAADVFYAGKAFLTTEIA
                     RWVRDEGLGLDVCTGGELALALHAGFPADRIALHGNNKSVAELAAALDAGVGRIVVDS
                     FEEIDRLTSLAQGRALRPRVLVRTTVGVEAHTHEFIATAHEDQKFGFSLAGGAAKEAT
                     RRILESDTLELVGLHSHIGSQIFDTAGFEVSAHRVVGLLREIRDDYGVELPELDLGGG
                     VGVAYVPTDDPPEISYIAAQLADIIERECNAASLPRPRLSVEPGRAICGPTTLTLYEV
                     GTIKPIEVGAGLVRTYVSIDGGMSDNIRTALYDATYSCTLANRASIAAPVLSRVVGKH
                     CESGDIVVRDAFLPADVAPGDLIAVPVTGAYCRSLASNYNYLGRPPVVAVRDGVARVI
                     VRRETIDDLLRLDVE"
     gene            complement(5317..6969)
                     /gene="argS"
                     /locus_tag="VGD55_09875"
     CDS             complement(5317..6969)
                     /gene="argS"
                     /locus_tag="VGD55_09875"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0870694.1"
                     /translation="MTPAQLSDSVIAAIAAAVAAGDLHVELPQAVTVERPKNREHGDY
                     ATNVALQLAKTAGRPPREIAELIAAQLRGTPGIAEVNVAGPGFLNVSLESAEQGEVAR
                     QIVEAGASYGHTKTQGGQRVNLEFISANPTGPLHLGHTRWAAVGDAIGRVLSAAGAEV
                     TREFYINDRGTQMDRFGASLEAAALSQPVPADGYHGDYIADLAAQIVRTRPSIVDLPE
                     GERVEAFREAGYELQLAEQKAVLDAFGTHFDVWYTERSLHSSGAVERGLARLREMGHV
                     YESEGAVWLRTTDFGDDKDRPLIKTNGELTYFASDTAYYVDKRARGFDVCIYLLGADH
                     HGYVQRLRTVAACAGDDPDYNIAVLIGQLVKILKGGVEIRLSKRAGEIVTLQEFVDLA
                     GVDAVRYSLARYPSDSPLTLDIDVITRKTNDNPVFYVQYAHARIASLLRNATELGIAR
                     GELDPALLTHGREVDLLGTLAEFPRVVASAAELREPHRVARYLEELAGVYHRFYDECR
                     VLPQGDDQVTDITRARLWLCEATRTVLANGLGLLGVGAPERM"
     gene            complement(7012..7422)
                     /locus_tag="VGD55_09880"
     CDS             complement(7012..7422)
                     /locus_tag="VGD55_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0870695.1"
                     /translation="MEHPRSRVLVVDDDPMIRHLITVNLELEGFDVTHADDGESCLRA
                     VELLRPDVIVLDVAMPLLDGLATAARLRADPSTSGIKIVMVSARAQQTDIRRGLEAGV
                     DVYLTKPFDPDVLIRAVRDLAETSLPDAEGPRIP"
     gene            7499..7570
                     /locus_tag="VGD55_09885"
     tRNA            7499..7570
                     /locus_tag="VGD55_09885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7532..7534,aa:Arg,seq:ccg)
     gene            complement(7626..8801)
                     /locus_tag="VGD55_09890"
     CDS             complement(7626..8801)
                     /locus_tag="VGD55_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY0870696.1"
                     /translation="MSKTRLVITAVVLEGRSQAEVARTYGVSKGWVSKLVARYRAEGE
                     AAFEPRSRRPKTSPHATPAATVELIVALRGRLAAAGLDAGPDTIGWHLAHEHKVTVSA
                     ATISRQLTKAGLVISAPTKRPRSSYIRFQADLPNQCWQSDFTHYRLSTGADTEIVSWL
                     DDCSRKALHASAHTAVTGPIVLATFRETVARYGVPASTLTDNGMVFTTRLAGGKGGRN
                     HLENELRRLHVQQKNSRPNHPTTCGKVERFQQTMKKWLRAQPDQPATIPELQALLDVF
                     IDLYNTGRPHRSLPHRATPEAIYDALPKALPANSRDTDTHLRIRHDRIDDSGVITVRH
                     AGKLHHIGIGRTHARTHVVALIADLDIRIVNAITGELLRHLNLNPDIDYQPQNTKNP"
     gene            complement(9009..>9353)
                     /locus_tag="VGD55_09895"
     CDS             complement(9009..>9353)
                     /locus_tag="VGD55_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870697.1"
                     /translation="TFPAPASGFSHLLFTVDGKWLVGSDTTAIYVWDGRTRQLVVSQL
                     SLPSNGATDALALAATDDHRLFVGTQTSLVVIDMDPSTWESQACSMAGRTLTRAEWTQ
                     FLPGRSYAPACR"
BASE COUNT         1552 a         3057 c         3143 g         1601 t
ORIGIN      
        1 tgttgtttcg gtcactcgaa tccttccgga aaaggcccac agcgtcggag ccggtgtcat
       61 gcacctgctc taactggagc cactttcaac tcgccgggcg gtgagccacg gcggtttcct
      121 gggagctatg gcgaagcgcc atagctgtta cacggggaag catcagcggc ttgcagaact
      181 gagtctcgat ccaccgtgaa tgctggtacg gcccggaatt gcggactgca ccagaaatca
      241 tggggtccac gcgcggagga agaccggaat tcggttctgt cggtttcggc agcccgggat
      301 gccggtcgaa ccggcgcgtc gtggggagca agcatccaga ccgggtaagg cgactggtgc
      361 aaaatcatcc tagcacccct gccccacccg aagacacctg ccacacctag aggctcggcg
      421 ccaccatcag acacatctga cgggacggcg cggggtgcag gttcgttagc ccgccagcgg
      481 catgacgcgc gcgccctggg tgtcgatcgc gatctgcgag gccgtgaacc agcgcgcgtc
      541 gaacgcggcg acggtctcgg cctgcgcggt cgtggtcaac gccagcaccg tggggcctgc
      601 gccggagatc accgcagcga tgccggcttc acgcaaccgc gccagcaatg ctgcgcttcg
      661 cggcatcgcc gaggcacggt attgctggtg cagggcgtct tgcgtcgcga cgagcagcaa
      721 ctcgggacgc cgggtcaacg cctgcgcgag caacgccgca cgtgccgcgt tcgcggcagc
      781 ttcgtggtgg ggcaccgact ctgggagcaa cttgcgggcc ttcgtcgtcg acgacttggt
      841 atcgggaacg aaggcaaccg cggcaatgct cttgtggacg tcgaggtgca gcgctcgagt
      901 caacagcgcg ggtgaacccg ttgttcccgg ttcggttccc ggctcggtcc aggccatcgt
      961 gaagccgccg gccaaacacg ccgccacgtt gtcggggtga ccttcgagct gcgcagcgag
     1021 tctgagcacg gcgtcgtcgg gcaatcgctc ggcgccgccg agcacgagct ctcgggccgc
     1081 cacgacgccc gccacgatcg cggccgccga cgagccaagg cctcggccgt gcggaatccg
     1141 gttcgcgcag acgatctcga ggccgcgggg ctgaccacca agagcgtcga acgtcgcccg
     1201 caccgcttgc acgacgaggt tgcgcttgtc gcgccgtacg tcgtcggcgc cctcgccggc
     1261 gacatcgacc acgaggcctg actccgcgac ccgcacgacg acgtcgtcgt acagcgcgag
     1321 cgcgaggccg aagctgtcga agcccgggcc caggttggcg cttgtggccg gcacccgcac
     1381 ccgggccggc gcggcgcgaa acgagacgcc cattacgcgt ccagaccgag gtggcgtgcc
     1441 gcgagcattg cgtcggccgc gatcgtgatc ggggccggtg cacccgagat cgcccactcg
     1501 gggtccttca agccgtggcc cgtcaccgtg cagaccaccc gcagacccgc gggcagcgtg
     1561 ccctgttcgt gagccatgag caggccagcc acgctcgcag cggaggcagg ctcgacgaag
     1621 acggcctcct tacttgccag caaccggtac gcggacagga tctgccggtc agtcaccgac
     1681 tcgatcgctc cccccgattg gtcgcgagca tcaagcgcgt tgacccagct cgccgggttg
     1741 ccgatgcgga tcgcggtggc gatggtcgag gggttctcga ccacatggcc gagcacgatc
     1801 ggcgccgaac ccgcagcctg gaatccgcgc atcttcggtc ggttggcagc gcgcccggcg
     1861 gctgcgtact cgacgtagcc cttccagtag gcggtgatat tgccagcgtt gccgaccggc
     1921 aggcagtgca cgtcgggcgc atcgccgagc gcgtcgacga tctcgaacgc cgcggtcttc
     1981 tgaccttgaa tgcggtcggg attgaccgag ttgaccaacg tgaccgggta gtcctgcgcg
     2041 agcttgctcg ctaatgacag gcagtcgtcg aagttgccgt caacctgcaa caacttggcg
     2101 ccgtgcacga gggcctgtgc gagcttgccg agcgcgatct tgcctcgcgg caccagcacc
     2161 gcgcacagca agccggcgcg cgctgcgtac gcagccgcgc tggcgctcgt gttgccggtc
     2221 gacgcgcaga tgactgcctt cgaaccgtcc tcgacggcct tgctgatcgc catcgtcatg
     2281 ccacggtctt tgaacgagcc ggtcgggttc gcgccttcga ccttcaagaa gacgtcgcag
     2341 ccggtgagcc cggaaagcac cggcgcgggc agcaacgggg tgccaccctc ttgcaacgtc
     2401 acgaccggcg tggcgtcggt aacgggcagc cactcgcggt attcctcgat cactccacgc
     2461 caacggggcc gggcactcat cggtcggtgt cctcgccctc gacccgcatc acgctcgcga
     2521 catcacgcac gatgtcgagc gtgcggaggt cggcgacggt cgctgacaag gcggcatcag
     2581 acgcggtgtg cgtcacgatc acgaggctcg cgtcgtcgcc gcgccctgat tgccgcaccg
     2641 cctggatcga gacgtcgtgg tcagaaaaca cattggccac ctgggcgagg acgccggctt
     2701 tgtcggccac gtcgaggctg atgtgatacc tcgtcagggt ctcgcccatc gagcgcaact
     2761 cgagatcggc gtacgcggat tcgcccggac caagcgcgcc cttcaccttg ttgcgcgcga
     2821 ccgcaacgat gtcgccgagc accgcactcg ccgtcggagc gccgcccgcg ccgcgaccgt
     2881 agaacatcaa ctggccggcc gcttcggctt cgacgaagac tgcgttgtaa gcctcgcgga
     2941 cgccggcaag cgggtgcgac cgcggaatca tcgccgggtg caccctgacg cttactccgc
     3001 gcccatcggc cgtgcgctcg cagatcgcga gcagcttcac cacgcaaccc atgtcgcgag
     3061 cactgagcac cgcggtcgcg gtgacgtcgc cgatcccctc tcgatggacg tcggcgatcg
     3121 tgacccgggt atgaaaggca agacccgcga ggatcgcagc tttcgcggcc gcgtcgaacg
     3181 cgtcgatatc ggcgcttgga tctgcctcgg catagccgag tgcggtcgcc tcgtcgagcg
     3241 cctcggcgaa cccggcgccg gtctcgtcca tccgggtcag gatgaagttg gtcgttccgt
     3301 tgacgatgcc gagcacccga tgcacggtgt cgcccgcgag cgattcgcgc agcggccgca
     3361 gcaacgggat cgcaccggcg acgcttgcct cgaagtacag gtcgacgccg tgcttgctcg
     3421 cggcgtcgta aagcgtcgcg ccgtcttctg ccagcagcgc cttgttggcg gtcacgaccg
     3481 atgctccgtg gctcatcgca gtaagcagca gcgatctcgc gggttcgata ccgccgatca
     3541 cctcgaccac gatgtcgatg tcgtcacgcg ccaccagagc cgctgcgtcg gtagtaaaca
     3601 gggctgggtc gaggtcgatg tcacgcttgc ggttgaggcg gcggactgcg acacccgcaa
     3661 gttcaagagg aacgccgacc ctggccgcga gatcgtcggc ctgctcggtc agcagcctcg
     3721 ccacctcgac tccgacgaca ccacagccga gcagggcgat ccgcagcggt cgcccgctca
     3781 tcgtgaatct ccgtccctca gtaggtggtc gtgccgtcgt tggtcgtgcc gtggctggta
     3841 gctcgtcgcc cgctcattct gccctgttcc cgccagcagc cgccgcgtca gtggtccgcc
     3901 acctcacgcg gcggcctcac tcgacgtcca gccgaaggag gtcatcgatc gtctcgcgcc
     3961 gcacgatcac cctggcgacg ccgtcgcgaa ccgcgacgac tggcggccgg cccagatagt
     4021 tgtagttgct cgccagcgat cggcagtagg cgccggtgac gggtaccgcg atcaggtcgc
     4081 cgggcgccac atcggcgggc aggaacgcgt cgcgcaccac gatgtcgccg gattcgcagt
     4141 gtttgccgac gacccggctc aagaccggcg cggcgatcga cgcgcggttc gccagcgtgc
     4201 aggaataggt cgcgtcgtag agcgcagttc ggatgttgtc gctcatgccg ccgtcgatgc
     4261 tgacgtaggt gcgaaccagc ccggcaccga cctcgatcgg cttgatcgtg ccgacctcgt
     4321 acaaggtcag tgtcgtcggc ccgcagattg cgcggccagg ctcgacactc agccgtgggc
     4381 gagggaggct ggcggcgttg cactcgcgct cgatgatgtc ggccaattgt gctgctatgt
     4441 aagagatttc aggcggatcg tcggtcggga cgtaagcgac tccgacgcca ccaccaaggt
     4501 cgagttcggg cagttcgacg ccgtagtcgt cgcgaatctc acgcagcagg ccgacgacgc
     4561 gatgcgccga cacctcgaag ccggcagtgt cgaagatctg cgagccaatg tgcgagtgca
     4621 ggccgaccaa ctcgagcgtg tcgctctcca gtatgcggcg ggtggcttcc ttggccgctc
     4681 caccggcgag ggaaaagccg aacttctggt cctcgtgtgc ggtcgcgatg aactcgtggg
     4741 tatgtgcctc gacacctacg gtcgtgcgca cgagaacacg ggggcggagt gccctgccct
     4801 gggcgagcga cgtcagccgg tcgatctcct cgaacgagtc gacgacgatc cgcccgacac
     4861 cggcatcaag tgccgcagcc agttcggcga ccgacttgtt gttgccgtgc agcgcgatcc
     4921 ggtcggcagg gaagccggcg tgcagggcaa gggccagctc gccaccggtg cagacgtcga
     4981 gcccgaggcc ttcgtcgcgc acccagcggg cgatctcggt cgtgagaaaa gccttgcccg
     5041 catagaagac gtcggcggcg gggaacgcgc cgacccagtt ctggcaacgc gcccggaagt
     5101 cggcctcgtc gacgacgtac gccggggtgc cgaagtcacg ggcaaggtcg gtggccgcca
     5161 ctcccgcgat tgtgagcacc ccggcgtccg acctggtgac gttcgacgac cagatcgtcg
     5221 gatcaagagc gttcacatcg gcgggcgcga ccggcgttgg tgacaacggc tcgcgggcat
     5281 cggcgtgcag cgctccggcc tcgtgggtgt gcacgactac atccgctcgg gtgcaccgac
     5341 gccgagcagg ccgaggccgt tcgccagcac ggtgcgggtc gcttcgcaca gccacagccg
     5401 agcccgcgtg atgtcagtaa cttggtcgtc accctgcggg agcacccggc actcatcgta
     5461 gaaacggtgg tacaccccgg cgagctcttc cagatagcgc gcgacgcggt gtggctcacg
     5521 tagctcggca gcgctggcga cgacacgcgg aaactccgcc aacgtgccga gcagatcgac
     5581 ctcgcgccca tgggtgagca atgcggggtc gagttcgccg cgcgcaatgc cgagttcagt
     5641 tgcgttgcgc aacaacgagg cgatccgtgc gtgcgcgtac tgcacgtaga acaccggatt
     5701 gtcgttggtc ttgcgagtga tgacatcgat gtcgagagtc aacggtgagt cgctggggta
     5761 ccgcgcgagt gagtagcgaa ccgcatcgac ccctgccagg tcgacgaact cctgcagcgt
     5821 gactatctct cccgcgcgct tcgatagccg gatctcgacg ccgcccttga gaatcttcac
     5881 cagctgcccg atgagcaccg cgatgttgta gtcgggatcg tcacccgcgc acgcagcgac
     5941 tgtccggagg cgttgtacgt acccgtggtg gtcggcgccg agaaggtaga tgcacacgtc
     6001 gaaaccacgc gcccgcttgt cgacgtagta cgcggtgtca gacgcgaaat acgtcagctc
     6061 gccgttggtc ttgatgagcg ggcggtcctt gtcgtcaccg aagtcagtgg tgcgcaacca
     6121 caccgcaccc tcggattcat agacgtggcc catctcgcgc agccgcgcca atccgcgttc
     6181 gaccgcaccc gacgagtgca acgagcgctc ggtgtaccag acgtcgaagt gggtaccgaa
     6241 tgcgtcgaga accgccttct gctcagccag ttgtagctcg tacccggcct cgcggaatgc
     6301 ctcgacccgc tcgccttctg gcaggtcgac gatgctgggg cgggtgcgca cgatctgcgc
     6361 ggcgaggtcg gcgatgtagt cgccgtggta accatcggcc ggaaccggct ggctcaaggc
     6421 cgcagcttca agagacgcac cgaaacgatc catctgcgta ccgcggtcgt tgatgtagaa
     6481 ctcgcgggtc acctcggccc cggctgcact gagcacccgc cctatggcat cgcctacagc
     6541 ggcccacctc gtgtgaccca gatgcagcgg cccggtcggg ttggcggaga tgaactcgag
     6601 gttcacccgt tgtccgccct gcgttttcgt gtgcccgtac gaggcaccgg cctcgacgat
     6661 ctgcctggcc acctcgccct gctcggcgct ttccaagctc acgttcagaa aaccgggacc
     6721 ggcgacgttc acctcggcga tgccaggcgt tccgcgaagt tgggccgcga tcagctcggc
     6781 gatctcgcgc ggtggccggc cagcggtttt tgccagctgg agagccacat tcgtcgcgta
     6841 gtcgccgtgc tccctgttct tcggccgctc gacggtgacc gcttgcggca gctcgacatg
     6901 gaggtcgcca gccgcgacgg cggcagcaat cgcagcgatc acagagtcgg acagttgagc
     6961 gggagtcacg ggcgcgaggc tatcgcagca tgagatccat tctcgtgcgc gttaggggat
     7021 tcgcggcccc tcggcgtccg gcaggcttgt ttctgcgagg tcacgaacgg cacggatgag
     7081 cacgtcaggg tcgaacggct tggtgaggta cacatcgaca ccggcttcga gcccgcgcct
     7141 gatgtcggtc tgctgcgccc tcgccgagac catcacaatc ttgatgccgg acgtcgaggg
     7201 gtcggcgcgc aacctcgctg cagtagccaa cccgtcgagg aggggcatcg ccacgtcgag
     7261 cacgatgaca tcagggcgga gcagttcaac tgcacgcagg cagctctcgc cgtcgtcggc
     7321 gtgggtgacg tcgaagcctt cgagctcgag gttcaccgtg atcaggtgcc ggatcatcgg
     7381 atcgtcgtcg acgacgagca ccctcgaccg agggtgttcc acgcggtcag attggtccga
     7441 accactcacg cgtcggacag tagtgcacgg gatccgctga taccctcggc acacttgagc
     7501 ccccgtagct caggggatag agcaccgccc tccggaggcg ggggcgcagg ttcgaatcct
     7561 gccgggggca ccgtgtcatc tctcaagaca tcggaaacct ctgaacctgc ggatgaaccc
     7621 gcgggtcagg ggtttttggt gttttggggt tggtagtcga tgtcggggtt gaggttgagg
     7681 tggcgcagta gttcgccggt gatggcgttg acgatgcgga tgtcgaggtc ggcgatgagt
     7741 gcgacgacgt gggttcgggc gtgggttcgg ccgatgccga tgtggtgcag tttgccggcg
     7801 tgacgcacgg tgatgacacc ggagtcgtcg atgcggtcgt gccggatccg caggtgggtg
     7861 tcggtgtctc ggctgttagc cggcagcgct ttgggcaacg cgtcgtagat cgcttctggg
     7921 gttgcccggt gcggtagtga gcggtgcggg cgcccggtgt tgtagaggtc gatgaagacg
     7981 tcgagcaacg cctgcagctc ggggatggtg gccggttggt cgggttgagc gcggagccat
     8041 ttcttcatcg tctgctggaa ccgctcgacc ttgccgcagg tggtcgggtg gttgggtcgg
     8101 gagttctttt gctgcacgtg cagacgacgc agctcgtttt ccaagtggtt gcgcccacct
     8161 ttcccgccgg ctaggcgggt ggtgaacacc atgccgttat ccgtcaacgt cgacgccggg
     8221 accccatacc tggcaacggt ttcgcggaac gtggcgagca cgatcgggcc ggtcaccgcg
     8281 gtgtgcgcgc tggcgtgcag cgctttgcgg gagcagtcat caagccagga cacgatctca
     8341 gtgtcggcgc cggtggaaag ccggtagtgg gtgaagtcgg actgccagca ctggttgggc
     8401 aagtcggctt ggaagcgaat gtaggaggag cgtggacgtt tcgtcggcgc ggatatcacc
     8461 aggcccgctt tggtcagctg ccggctgatc gtggcggccg acaccgtcac cttgtgctcg
     8521 tgggccaggt gccagccgat cgtgtccggg ccggcgtcca gacccgccgc ggccagccgc
     8581 ccgcgtagcg cgacgatcaa ctccaccgtc gccgccggcg ttgcgtgcgg agaggttttc
     8641 ggccgccgcg accgcggctc gaacgctgcc tcgccttcgg cccggtaacg ggccaccagc
     8701 ttgctcaccc agcccttgga caccccatag gtgcgggcga cctcagcctg agaacgaccc
     8761 tccagaacaa ctgcagtgat caccagacgc gttttgctca tcgcgcagca tcagcgcacc
     8821 cggtttccta tgtcgtgaga gacccgtttc ctatgtcctg atgcagaaca ctgccgggcg
     8881 cgctttttca tttgaccgcc aagaccttcg atcggttctt cgactacttc cacgaggact
     8941 acggcgtatg gccgctcact tatcgagccg gaaggtgtct gggcgggcgc aggctacgcc
     9001 gacgaggtct agcggcacgc aggtgcgtag gagcgaccgg gcaagaattg agtccactcc
     9061 gcgcgcgtca atgtccgccc ggccatgctg cacgcctggc tttcccaagt ggatggatcc
     9121 atgtcgatca caacgaggct ggtctgcgta ccgacgaaca agcggtggtc atcggtggca
     9181 gcgagcgcaa gggcatcggt tgcaccgttc gacggcagcg atagctgtga gaccacgagc
     9241 tgccgggttc gaccgtccca tacgtagatc gccgttgtat cgcttccaac taaccacttg
     9301 ccgtcgaccg taaacaggag gtggctaaag ccacttgccg gggcgggaaa cgt
//
LOCUS       DASWVF010000558         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_219306, whole genome shotgun sequence.
ACCESSION   DASWVF010000558 DASWVF010000000
VERSION     DASWVF010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219306"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1688)
                     /gene="groL"
                     /locus_tag="VGD55_09900"
     CDS             complement(63..1688)
                     /gene="groL"
                     /locus_tag="VGD55_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631882.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0870698.1"
                     /translation="MSKIIAYDETARRALERGMNQLADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPMALKRGIEAAVASVSAALLALAKDVETREQIASTASISAADPAIGEMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGHLSAYFVTDTERMETVLDDPYI
                     LVVNSKISSVKDLLPLLEKVMQSGKPLAIIAEDVEGEALATLVVNKIRGTFRSVAVKA
                     PGFGDRRKAMLGDIAILTGAQVISEEVGLKLETAGLDLLGKARKFVTTKDETTIIEGA
                     GDADAIAGRVNQIRAEIEKSDSDYDKEKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALLQAGRTAFEKLNLEGDEATGARIVEIALEAPLK
                     QIAINAGLEGGVVVEKVRNLEPGWGLNAATGEYVDLIKAGIIDPAKVTRSALQNAASI
                     AALFLTTEVVIAEKPERNPAPAGGGGGGGDMDF"
     gene            1911..2738
                     /locus_tag="VGD55_09905"
     CDS             1911..2738
                     /locus_tag="VGD55_09905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870699.1"
                     /translation="MTDDWRAINLANWESRVSLHIGPGGYDLDAFDDPTHLSTVVRYD
                     LPRLGRLDGLDVVHLQCHLGTDTVSLARLGPRSTTGLDFSPSALASARELATRASADV
                     TFVDGDAYDAVELLGANRFDLVYTGIGAICWLPNIRQWAAVVAGLLRPDGRLFMREGH
                     PVLWSLSDPRPDGLLVVEYPYFENEGVRFSEETSYQGTGTLSAPESVSFNHGLGEIFT
                     ALQEAGLKMTSFEEHREVPWNPLGEAMVESPDFPGEFVLAEGRDRLPLSYTIQAVRS"
     gene            complement(2735..4039)
                     /gene="thrC"
                     /locus_tag="VGD55_09910"
     CDS             complement(2735..4039)
                     /gene="thrC"
                     /locus_tag="VGD55_09910"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0870700.1"
                     /translation="MVRGEDEERGFTVSAIQSPPAKTVLGSATALSCRECGEQYPISP
                     RYACEMCFGPLEVAYDYAGVTRESIEGGPHNIWRYKQLLPVPSNVADTPNLEPGMTKL
                     VLANNLARELGMRKLWVKDDSGNPTHSFKDRVVAVALAAARELGFSVLACPSTGNLAN
                     AVAAAAARAGIKSVVFIPADLERQKVVTTAVYGGTLVAVQGNYDDVNRLASEIAGDEE
                     DWAFVNVNVRPFYAEGSKTLGYEIAEQLGWRLPEQVVIPIASGSQLTKVDKAFTEFGK
                     LGLVDATPYRVYGAQAFGCSPVSKAFKAGYDVVQPVRPDTIAKSLAIGNPADGPYVLD
                     VCRRTGGLVEDVTDDEIVDGMRLLARTEGIFGETAGGVTVGVLRKLLAAGQLDPDAET
                     VIFNTGDGLKTLDAVASVVKPSVTIPPSAEAFRAFLDAEGLA"
BASE COUNT          636 a         1359 c         1301 g          750 t
ORIGIN      
        1 aacaaagcgg tggggcggcg cctggtttcc cggacgccgc cccagccacg atcgagggac
       61 tcttagaagt ccatgtctcc gccgccgcca ccgccgccgg caggagcagg gttgcgctcc
      121 ggcttctcgg cgataacgac ctcggtggtg aggaagagcg ccgcgatcga cgccgcgttc
      181 tgcagtgccg atcgggtgac cttcgcgggg tcgatgatgc cggccttgat caggtcgacg
      241 tactcaccgg tcgccgcgtt gaggccccag ccgggctcga ggttgcgcac cttctcgacc
      301 acgacgccac cttcgaggcc tgcgttgatc gcgatctgct tcagcggtgc ctcgagggcg
      361 atctcgacga tgcgagcgcc agtggcctcg tcaccttcga ggttgagctt ctcgaatgcg
      421 gtgcgaccgg cctggagcag cgcgacgcca ccaccggcga cgatgccctc ttcgacggct
      481 gccttcgcgt tgcggaccgc gtcttcgatg cggtgcttgc gctccttgag ctcgacctcg
      541 gtggccgcgc ccgccttgat gactgcaacg ccgccagcca acttggccaa ccgctcctgc
      601 agcttctctt tgtcgtagtc ggagtcgctc ttctcgatct ccgcacgaat ctggttgacc
      661 cgaccggcga tggcgtcggc gtcgccggca ccttcgatga tcgtcgtctc gtccttggtc
      721 gtgacgaact tgcgggcctt gccgagcagg tcgaggccag cggtctcgag cttcagaccg
      781 acttcctcgc tgatgacctg cgcgccggtg aggatcgcga tgtcgccgag catggccttg
      841 cggcggtcac cgaagccggg cgccttgact gcgaccgaac ggaacgtacc gcggatcttg
      901 ttcacgacca gggtcgcgag ggcttcgcct tcgacgtcct cggcaatgat cgcgagcggc
      961 ttgccgctct gcatgacctt ctcgagcagc gggagcaggt ccttgacgga cgagatcttg
     1021 ctgttcacaa cgaggatgta cgggtcgtcg agcacggtct ccatgcgctc ggtgtcggtg
     1081 acgaagtacg ccgacaggtg acccttgtcg aagcgcattc cctcggtgag ctcaagctcg
     1141 agaccgaagg tgttgctctc ctcgacggtg atgacgcctt ccttgccgac cttgtccatc
     1201 gcttcggcga tcatctcgcc gatcgctggg tcagccgctg agatggacgc ggtcgaggcg
     1261 atctgctcgc gggtctcgac gtccttcgcc agggcaagta gcgccgccga aacactcgcg
     1321 actgcagctt cgatgccacg cttcagtgcc atcgggttcg cgccggccgc gacgttgcgc
     1381 aggccttcac gaacgagcgc ctgggcaagc acggtcgcgg tcgtcgtgcc gtcaccggcg
     1441 acgtcgtcgg tcttcttcgc gacttccttg accagctctg cgccgatctt ctcgtagggg
     1501 tcttcgagct cgatttcctt cgcgatgctg acgccgtcgt tggtgatcgt gggggcgccc
     1561 cacttcttct ccaacacgac gttgcggccg cgcgggccga gcgtcacttt cacggcgtcg
     1621 gcgagctggt tcataccgcg ctcgagggcg cgacgagcag tctcgtcata cgcgatgatc
     1681 ttggacatgc ggatggtcct cccggtcggg aaacatagct acggccgagc tcgacgcccg
     1741 cgccggccga ccacacctcg ccgacgtcat gtccgccggc tcggcagggt ctcgaacggc
     1801 ccggccagga aactgtcact ctcacgctca gagtgctagc tcatgtttag cactcagacc
     1861 acccgagtgc aaccggcgcc gggtgaacct tggcctctag attgagcgtc atgacagacg
     1921 actggcgggc gatcaatctc gcgaattggg agtcacgggt atcgctccat atcggtcctg
     1981 gcggctacga cctcgacgcc ttcgacgatc cgacccacct gtcgacggtg gtgcgctacg
     2041 acctccctcg cctcggtcgc ctcgatggcc tcgatgtcgt ccaccttcag tgccacctgg
     2101 gcaccgacac ggtctccctc gcccggctgg gcccgcgctc gacaaccggc ctcgacttct
     2161 cgccgtccgc cctcgcatcg gctcgagagc tcgccacgcg tgcgagcgcc gacgtcacct
     2221 tcgtcgacgg cgatgcgtac gacgcggtgg agttgctcgg cgcgaaccgg ttcgacctcg
     2281 tctacaccgg catcggcgcc atctgctggc tgccgaacat ccgccagtgg gcagccgtcg
     2341 tggcggggtt gctgcgtcct gatggccggc tgttcatgcg cgaggggcat ccggtgctgt
     2401 ggtcgctgag tgatccgcgg cccgacggtc tgctcgtggt cgagtatccg tacttcgaaa
     2461 acgagggagt ccgcttcagc gaggaaacga gctaccaggg gaccggcacc ctcagcgcgc
     2521 ccgagagcgt gagcttcaac catggcctcg gcgagatctt cacagcgcta caagaagccg
     2581 gcctcaagat gacgagtttc gaggaacatc gcgaggtccc gtggaaccct ctgggcgagg
     2641 cgatggtcga aagtccagac ttcccgggcg aattcgtact cgccgagggg cgcgatcggc
     2701 tgccgctcag ctacacgatc caagccgtgc gcagctaggc cagcccctcg gcgtccaaga
     2761 aggctcgaaa tgcttcggcg gacggcggga tggtgaccga gggcttgacg accgacgcca
     2821 ccgcgtcgag ggtcttcaga ccgtcgccgg tgttgaagat gacagtctcg gcgtcggggt
     2881 cgagctggcc ggccgcaagc aacttacgca gcacgccgac cgtgacgccg cccgccgtct
     2941 cgccgaagat gccctcggtg cgggcgagta gccgcatgcc gtcgacgatc tcgtcgtcgg
     3001 tgacatcctc gacgagccca ccggtgcggc gacagacgtc gagcacatac ggtccgtcag
     3061 ccgggttgcc gatggcgagt gatttggcga tcgtgtcggg tcgcaccggc tgcacaacgt
     3121 cgtagcccgc cttgaacgcc ttcgacaccg gcgagcaacc gaacgcctgc gcgccgtaga
     3181 ctcggtacgg cgtcgcgtcg accaagccga gcttgccgaa ctcggtgaaa gccttgtcga
     3241 ccttcgtcag ctgcgagcct gaagcgatcg ggatcaccac ctgctcaggt aaccgccagc
     3301 cgagttgctc ggcgatctca tagcccaatg tcttcgagcc ctcggcgtag aacggccgca
     3361 cgttgacgtt gacgaacgcc cagtcttctt cgtcgcccgc gatctccgat gcgagccggt
     3421 tgacgtcgtc gtagtttccc tggacggcga ccagcgtccc gccgtagacg gcggtcgtca
     3481 cgaccttctg ccgctcgagg tccgccggga tgaacaccac cgacttgatg ccggcgcggg
     3541 cagccgcggc agcaaccgcg ttcgccagat tgcccgtcga cgggcacgcc aacactgaga
     3601 atccgagttc gcgcgcagca gcgagcgcga cggcgacgac gcggtccttg aacgagtgcg
     3661 tcgggttgcc gctgtcgtcc ttcacccaga gcttgcgcat gccgagctcg cgggcgagat
     3721 tgttggcgag caccagtttt gtcatgccag gctcaagatt tggtgtgtcg gcgacgttcg
     3781 acggcaccgg cagtagttgc ttgtagcgcc agatgttgtg cggcccgcct tcgatcgact
     3841 ctcgcgtcac gccggcgtag tcgtaggcca cttcgagcgg gccgaagcac atctcgcagg
     3901 catagcgggg gctgatcgga tactgctcgc cacactcgcg acacgacagc gcggtggcgg
     3961 agcctaagac agtttttgcg gggggcgact ggatcgcgga cacggtgaag cctctctcct
     4021 catcttcccc gcggaccatc cacggg
//
LOCUS       DASWVF010000559         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_448790, whole genome shotgun sequence.
ACCESSION   DASWVF010000559 DASWVF010000000
VERSION     DASWVF010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448790"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2178
                     /gene="topA"
                     /locus_tag="VGD55_09915"
     CDS             <1..>2178
                     /gene="topA"
                     /locus_tag="VGD55_09915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY0870701.1"
                     /translation="DETRRLVIVESPAKAKTIAGYLGRNYIVESSIGHIRDLPTSAAD
                     IPAKYKKEPWSRLGVDVDHGFEPLYVVNADKRQQVAKLKAALAGVDELYLATDEDREG
                     EAIAWHLLEVLNPQVPVHRMVFHEITRDAIREAAQHPRELNQALVDAQETRRILDRLY
                     GYEVSPVLWKKVMPRLSAGRVQSVATRMVVDRERDRIAFRSASYWDVNALFATTPTFE
                     ATLAAVDGQRVAQGRDFDSLGVLKTKNLVHLDERQATELAGGLSNTEFVVRSVESKPY
                     RRSPAPPFMTSTLQQEASRKLRFSSQTTMRIAQRLYENGHITYMRTDSTTLSETALNA
                     ARNQVRELYGSEFLPDVPRRYEKKVKNAQEAHEAIRPSGDAFATPGELAGQISGDELR
                     LYELIWQRTVASQMTDATGNTVTMRLGGTSRTGRDAEFSASGTVIVHQGFLKVYVESS
                     DDDAADDAGNERRLPNLSEGDTARATSLEAVGHSTNPPSRYTEASLVRALEERGIGRP
                     STFASIMSTILDRGYIFKKGTALVPTFLAFAVVQLLERHFGDLVDYDFTAEMEEDLDR
                     IADGETARTAWLTHFYFGGPLDGVAATHSPKGPNGHDDNPGLKAMVHDRLGEIDARDV
                     SSFPLGDSGIVVRVGRYGTYLERDGQRANLPDGVAPDEVTQEYAQALLDKPSGDRPLG
                     VDPISGRELVAKDGRYGPYVTEILPEDSKAKPRTASLFQSMSLD"
BASE COUNT          416 a          744 c          697 g          321 t
ORIGIN      
        1 gacgagaccc ggcgtctcgt catcgtcgaa tccccggcga aggccaagac gatcgcgggc
       61 tacctcggcc ggaactacat cgtcgagtcg agcatcgggc acattcgcga cctcccgacc
      121 tcggccgccg acatcccggc gaagtacaag aaggagccgt ggtcacgcct cggcgtcgac
      181 gtcgatcacg gattcgaacc cctctacgta gtcaacgccg acaagcggca gcaggtcgcg
      241 aagctgaagg cggcgctggc cggagtcgac gagctttacc tcgccacaga cgaggaccgc
      301 gagggcgagg ccatcgcctg gcacttgctc gaggtgctga acccgcaggt gccggtgcac
      361 cggatggtct ttcacgagat cacccgcgac gcgatccgcg aggcggccca gcatccgcgc
      421 gagctgaacc aggcgctggt cgacgcgcaa gagacccggc gcatcctcga ccgcttgtac
      481 ggctacgagg tcagcccggt gctgtggaag aaggtcatgc cgcggttgag cgccggccgg
      541 gtgcaaagtg tcgcaacccg catggtcgtc gaccgcgaac gcgaccgcat cgctttccgc
      601 agtgcgtcgt actgggacgt caacgccctc ttcgccacca cccccacgtt tgaggccacc
      661 cttgccgcgg tcgatggcca gcgcgtcgcg cagggtcgcg acttcgactc actcggcgtg
      721 ctgaagacga agaacctggt gcacctcgac gagcgccaag caactgagct tgccggcggg
      781 ttgagcaaca ccgagttcgt ggtccgcagc gtcgagagca agccgtaccg gcgcagcccg
      841 gccccgccgt tcatgacgag cacgcttcag caagaggcca gccgcaagtt gcgattcagc
      901 agccaaacca cgatgcggat cgcccagcgc ctctacgaga acggccacat cacctatatg
      961 cggaccgact cgacgaccct gagcgagacg gctctcaatg ccgcccgcaa ccaggtgcgc
     1021 gagctgtacg ggagtgagtt cctccccgac gtcccgcgta ggtatgaaaa gaaagtcaag
     1081 aacgcgcaag aggcgcacga ggcgatccgg cctagcggtg acgcttttgc cacgcccggt
     1141 gagctcgcgg gtcagatttc gggcgacgag ttgcggcttt acgagctgat ctggcagcgc
     1201 accgtcgcct cacagatgac cgacgcgaca ggcaacaccg tgacgatgcg tctgggtggc
     1261 acgagccgca ccggccgcga cgccgagttc tcggcatccg gcacggtgat cgtgcaccag
     1321 ggtttcttga aggtctacgt cgagagcagc gacgacgatg ccgccgacga cgcgggcaac
     1381 gagcgcaggc tgcccaacct gtcggagggt gacaccgcgc gagcgacctc gctcgaagcg
     1441 gtcgggcaca gcacgaaccc gccgtcccgc tacaccgagg cgagcctggt gcgagcgctc
     1501 gaagagcgtg ggatcggtcg cccgagcaca ttcgccagca tcatgagcac gatcctcgac
     1561 cgcggttaca tcttcaagaa gggcaccgcg ctcgtcccga ccttccttgc gttcgccgtg
     1621 gtgcagttgc tcgagcgcca ctttggcgac ctcgtcgact acgacttcac cgccgagatg
     1681 gaagaagacc tcgaccgcat cgccgacggc gagacggccc gcacggcatg gctgacgcac
     1741 ttctacttcg gcggtccgct cgacggtgtc gccgccacgc acagtcccaa gggaccgaac
     1801 ggtcacgacg acaaccccgg actcaaggcg atggtgcacg accggctcgg cgagatcgac
     1861 gctcgcgacg tcagctcgtt cccgctcggc gactcgggca tcgtggttcg cgtcggccgg
     1921 tacggcacct acctcgaacg tgatggccag cgggcgaatc tgcccgacgg ggtggcgcct
     1981 gatgaagtga cacaggagta tgcccaggcg ctgctcgaca agccgagtgg tgaccggccg
     2041 cttggtgtcg acccgatctc gggccgcgag ctcgttgcaa aagacggccg ctacggcccc
     2101 tacgtgacag agatcctgcc tgaagattcg aaggccaagc cccgcaccgc cagcctcttc
     2161 cagtcgatgt cgctcgac
//
LOCUS       DASWVF010000560         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_372007, whole genome shotgun sequence.
ACCESSION   DASWVF010000560 DASWVF010000000
VERSION     DASWVF010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372007"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VGD55_09920"
     CDS             <1..1178
                     /locus_tag="VGD55_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEY0870702.1"
                     /translation="RDIETLMSDPATKIIVCCGSGGVGKTTLAAALGVRAAEHGRRVV
                     TLTIDPARRLAQSLGLEELDNVPRKVAGVRSSKKSGGGELHAMMLDMKRTFDEIVEAH
                     ATPAKAAQILANPFYQSLSSSFAGTQEYMAMEKLGQLRASGKWDLIVVDTPPARSALD
                     FLDAPARLARFLDGRFIRMLLVPAKVGGRGYAKVMQVGLGLVTSTMTKLLGTQLLTDV
                     QTFVAAMDTMFGGLRQRADATAKLLRAPGTIFVVVAAPEPDALREASYFVDRLVEEDM
                     PLAGVVVNRVHRTTVPQITAVQALSAADALDDDADTGSNTDGALTAGVLRVHAERMAV
                     QAAEQRRVDGFALAHPNVALASARALAQDVHDLTGLRELGAALCDRGRADAGASAGS"
     gene            complement(1164..1472)
                     /locus_tag="VGD55_09925"
     CDS             complement(1164..1472)
                     /locus_tag="VGD55_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY0870703.1"
                     /translation="MAWTTDWTTRAGCRGTDPDALFVQGAAQNRAKTLCFGCPVRAEC
                     LADALDNRVEFGVWGGLTERERRALLRSRPNVTSWQRLLSTAMSEFERSLSEGYSATG
                     "
     gene            1671..>1877
                     /locus_tag="VGD55_09930"
     CDS             1671..>1877
                     /locus_tag="VGD55_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870704.1"
                     /translation="MARTAREGRIGFGGRLWRALALGVLSILVGLLVAGLALPAAGGL
                     GTATRNAIGTITFSDLPASLQTPPL"
BASE COUNT          294 a          634 c          628 g          321 t
ORIGIN      
        1 ctcgcgatat cgagacactc atgtccgacc cggccacgaa aatcatcgtg tgttgcggct
       61 ccgggggcgt tggtaagacg acactcgcgg ctgccctcgg cgtccgcgct gctgaacatg
      121 ggcgccgcgt cgtcaccctc acgatcgatc cggccaggcg gctggctcaa tcacttgggc
      181 tcgaagagct cgacaacgtt ccgcgcaagg tggcaggcgt gcgttcgtcg aagaagtccg
      241 gcggcggcga gctgcacgcg atgatgctcg acatgaagcg cacgttcgac gagatcgtcg
      301 aggcgcacgc gacgccggcg aaagccgcgc agattctggc gaacccgttc tatcaatcgc
      361 tgtcgtcgag cttcgcgggg acgcaggaat acatggcgat ggagaagctc gggcaactgc
      421 gtgcgtcagg caagtgggat ctcatcgtcg tcgacacccc gccggcacgt tcggcgttgg
      481 acttcctcga cgccccggcg cgactcgcgc gctttctcga cggccggttc atccgcatgc
      541 tgctcgtccc tgcgaaggtc ggcggtcgcg gatacgcgaa ggtcatgcag gtaggactgg
      601 gtctcgtcac cagcacgatg accaagctgc tcggaactca gctgctgacc gacgtccaga
      661 cctttgttgc tgcgatggac acgatgttcg gcggcttgcg ccaacgagcc gacgccaccg
      721 cgaagctgct gcgcgcccct ggcacgatct tcgtcgtcgt ggctgcgcct gagcccgacg
      781 cccttcgcga ggcttcgtac ttcgtcgatc ggttggtcga ggaagacatg ccgttggcgg
      841 gcgtcgtcgt gaaccgcgtg catcgcacga cggtgcccca gatcaccgcc gtgcaggcac
      901 tgtctgcggc cgatgcgctc gacgacgacg ctgataccgg cagcaacaca gacggcgcgc
      961 tcacggccgg cgtcctgcgc gtgcatgccg agcgcatggc ggtgcaagcc gccgagcagc
     1021 ggcgggtcga cggtttcgcg ctggcgcacc cgaacgtggc gctggcgtcg gcgcgggcac
     1081 tggcccaaga cgtgcacgac ctgacgggct tgcgtgaatt gggtgcggcg ctgtgcgatc
     1141 gcggtcgagc tgacgctggg gcctcagccg gtagctgagt agccctcgga gaggctccgc
     1201 tcgaactcgc tcatcgcggt gctcagaagg cgctgccacg acgtgacgtt cgggcggctg
     1261 cggagcaggg cgcgacgctc tcgctcggtg agcccgcccc acacgccgaa ctcaacccgg
     1321 ttgtcgaggg catctgccag gcattcggcg cgcaccgggc agccgaagca caaggtctta
     1381 gcgcggttct gtgcggcgcc ctggacgaac aacgcgtccg ggtctgtgcc gcggcatcct
     1441 gcgcgagttg tccagtcagt ggtccaagcc atcgggcgcc ccgttcgttg ccgacttcag
     1501 accgatcacg tcaccccccc ggtgcccgct cgatctgctt cgaaggtacg gacagtgacg
     1561 aggcgaacaa aagcctccgt tcggcttaat cggggtcgtc cgggtggacc gtggcacggc
     1621 caattgggtt agcgaggcct agtcccccat gcagcggtta ccctgcacta atggctagaa
     1681 ccgcacgaga agggcgtatc ggcttcggcg gacgcctgtg gcgtgccctc gccctcggcg
     1741 tgctgtcgat tctggtcggg cttctcgtcg cgggccttgc cctgccagcc gcgggcgggc
     1801 tcggcaccgc gacccgcaat gcgatcggca ccatcacctt ctccgacctc cccgcgtcgc
     1861 tccagacacc cccgctc
//
LOCUS       DASWVF010000561         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_193803, whole genome shotgun sequence.
ACCESSION   DASWVF010000561 DASWVF010000000
VERSION     DASWVF010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193803"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGD55_09935"
     CDS             complement(<1..272)
                     /locus_tag="VGD55_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870705.1"
                     /translation="MLVAVRGVSLSVAPGEKIAIVGANGAGKTTLLRTIAGAHPLSSG
                     SISLDGDDVSKLPAYKRVAAGIAMVPEGRKLFADMTVRENLQLAGR"
     gene            complement(311..1033)
                     /locus_tag="VGD55_09940"
     CDS             complement(311..1033)
                     /locus_tag="VGD55_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870706.1"
                     /translation="MDVLVGRGLVKRYGDLVVLDDVDFTLGAAEAVGIVGPNGAGKTT
                     LLSVLAGSVELTAGEVLLRDQVVTNLRPDVRCGRGLARAFQVPRPFGGMTVLENVLVG
                     ATAGGHLRGIPAYDRSIDALVTCGLDGLSNRRADSLGLLHRKRLELARALATDPAVLL
                     LDEIGGGLTDAEAAQLVVTIQQLHAQGIAIVWIEHIVHILVQVVDRLVCMDAGKVIAD
                     GPPEEVLQDAAVVSAYLGRGPQ"
     gene            complement(1096..2427)
                     /locus_tag="VGD55_09945"
     CDS             complement(1096..2427)
                     /locus_tag="VGD55_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0870707.1"
                     /translation="MSGSEMSARAMSLPGPSRRQFLQGAGFGLLGLAVGSPLLAACSS
                     SGSKAGSGGSLKIGFVSPITGPAAGFGEPDGYVLSLARKAFANGLTIGGKNYAITIVD
                     KDGQSDPQRSAQVANDLINSDKVDLMLTTSTPETVNPVSDACEAAGVPCVSTVVPWEA
                     WYFGRGAKPGAPSPFKFTYHFCFGVQQFDNAYTTMWPQVSTNKKVGVMWPNDADGNAI
                     RSALGPLLTKAGYTIVDPGAYTDGTNDYSSQIAKFIAEDCEIFNTFPLPPDFATFWQQ
                     AAQQGYKPKIAQIAKTGLFPSQVEALGAIGVGLAGGVYWDPTWPYNSSLTKITSKQLG
                     DGYQTTAKKQWNQQLGASLALFDVAAAALQASADPKDKAGVAKSISTLTVDTPVGTLA
                     WGKGPVPNVVATPIIGGQWIKGTGSYPLDFVICENSSDPKVPIAAKLQPYV"
     gene            2571..3434
                     /locus_tag="VGD55_09950"
     CDS             2571..3434
                     /locus_tag="VGD55_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY0870708.1"
                     /translation="MFGVVRIVATSGSARSTRPAIASEYSQSLERGLAILSAFSETRP
                     LLGTAELGRCVELNKSTAHRYISTLTELGYLQQDQGTRKYRLGPRVVNLGFAAINSME
                     ITKIAAPHMQALSDETGLAVSMAVLDGVDIVYTQRVRSNSAGFTGIDLNLHIGSRLPA
                     YCTSMGKVLLAHQRPEVLKSILDRTDLARRGPNTLTAREALIAALRRVRQIGLAVNDQ
                     ELAPGLRSLAVPIRDGFGEVIAALNIAVHMSIGNASLESTVSRLEGPLRKAAGEISSR
                     MGYRESPAVYR"
     gene            3511..>4607
                     /locus_tag="VGD55_09955"
     CDS             3511..>4607
                     /locus_tag="VGD55_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0870709.1"
                     /translation="MTEQPLLAQDIWRGKIFVGGSWAPSARGATAAVIEPATGAELGS
                     IGEAGADDVAVAAESAAAAQVAWAATPYPARAAVLRRAGDLWAEHAEEISWWNIREVG
                     TIPGMAGFALHVASQECYEASALPSRAYGELIPTEEPRLSMARRVPAGVVGVISPFNV
                     PIILGIRSVAPALALGNAVILKPDPRTSVTGGVTIARIFEEAGLPAGVLQMVPGGVEV
                     GEALVTHPLVRVISFTGSTSAGRRVGELAARHLKRVHLELGGNSAMIVLDDADVDAAV
                     GLAAWGSFFHQGQICMTTGRHIVAASLYDDFVERLAARAQKLPVGNPATDQVALGPVI
                     DARQRDKIHSLVTQSQSAGARVAAGGTFEGL"
BASE COUNT          763 a         1538 c         1532 g          774 t
ORIGIN      
        1 tgccggccgg cgagctgcaa gttctcgcgc acggtcatgt cggcgaagag cttgcgcccc
       61 tcgggcacca tcgcgatgcc tgccgcgacc cgcttgtagg ccggcagctt gctgacgtcg
      121 tcgccgtcga gggagatcga gccggaagaa agtggatggg cgccggcgat cgtgcggagc
      181 agcgtcgtct tgcctgctcc gttggcgccg acgatcgcga tcttctcgcc cggcgcgacg
      241 ctcagcgata cgccgcgcac cgcgacgagc aaaccgtgag aggcgacgag gtggtcgaca
      301 gcgagcaggc tcactgcggc ccccggccga gataggcgct gaccaccgca gcgtcctgaa
      361 ggacctcttc gggagggccg tcggcgatga ccttgcctgc gtccatgcac acgagccggt
      421 cgacgacctg cacgaggatg tgcacgatgt gttcgatcca gacgatggcg atgccctgcg
      481 cgtgtaactg ctgaatggtg acgaccaact gcgcggcctc ggcgtcggtc aatccgccgc
      541 cgatctcgtc gagcagcagg accgcgggat cggtggcaag ggcgcgcgcg agttcgagcc
      601 gcttgcggtg tagcagcccc aaactgtcgg cgcggcggtt cgacaaccca tcgaggccac
      661 acgtgaccag cgcatcgatg gaacggtcgt acgcgggtat gccacgaagg tgcccgccgg
      721 ccgttgcgcc gacgagcacg ttctccaaga ccgtcatgcc accgaacggc cgcggcacct
      781 ggaacgcacg tgccaatccg cgcccgcatc gcacgtcggg ccgaaggttc gtcaccacct
      841 ggtcgcgcag caacacctcg cccgcggtca gctcgaccga cccggcgaga acactcagca
      901 acgttgtttt gccggccccg ttcgggccga cgatgccgac tgcctcggca gcaccgaggg
      961 tgaaatcgac atcgtcgaga acgacgagat cgccgtaacg cttgaccagt ccgcggccga
     1021 ccaggacgtc caagccggcc ccacttccga catcaaccgc gacgctgcta tcagtgctat
     1081 ccgcgtgcct gccggtcaca cgtagggctg cagcttagcc gcgatcggaa ccttgggatc
     1141 ggaggagttt tcgcagatga cgaagtcgag cgggtagctt ccggtgccct tgatccactg
     1201 gccgccgatg atcggggttg cgacgacatt gggcaccggg cccttgcccc aggcaagcgt
     1261 cccgaccggc gtgtcgaccg tcagggtcga gatgctcttc gcgaccccgg ccttgtcttt
     1321 cgggtctgcg ctggcctgca acgccgcggc ggcgacgtcg aacagcgcca ggcttgcacc
     1381 gagctgctgg ttccactgct tctttgccgt tgtctggtag ccgtcgccga gttgcttcga
     1441 cgtgattttc gtgagcgacg agttgtaggg ccaggtcgga tcccagtaga cgccgcccgc
     1501 gagcccgacg ccgatcgcac cgagcgcctc gacctgcgag gggaacaagc cggtcttcgc
     1561 gatctgggcg atcttcggct tgtaaccctg ctgcgcggcc tgctgccaga acgtcgcgaa
     1621 gtcaggcggc aacgggaacg tgttgaagat ctcgcagtct tcggcgatga acttcgcgat
     1681 ctgcgacgag tagtcgttgg tgccgtcggt gtaagcaccg gggtcgacga tcgtgtagcc
     1741 ggccttggtc agtagcggcc cgagcgccga gcgaatcgcg ttgccgtcgg cgtcgttggg
     1801 ccacatgacg ccgaccttct tgttggtcga cacctgtggc cacatcgtcg tgtaggcgtt
     1861 gtcgaactgc tggacgccga agcagaagtg gtaggtgaac ttgaacggcg acggcgcacc
     1921 cggctttgct ccacgaccga agtaccacgc ctcccacggc acgaccgtcg agacgcacgg
     1981 cacaccggct gcctcgcacg cgtcggacac ggggttgacc gtctcgggcg tcgaggtggt
     2041 gagcatcagg tcgaccttgt cgctgttgat gaggtcgttc gcgacctggg cactgcgctg
     2101 cgggtctgac tggccgtcct tgtcgacgat cgtgatcgcg tagttcttgc caccgatcgt
     2161 caagccgttc gcgaacgcct tgcgcgcgag gctcagcacg taaccgtcag gctcgccgaa
     2221 gcccgcggcc gggccagtga tcgggctcac gaacccgatc ttcaggctgc cgccggagcc
     2281 tgccttcgac cccgagctac tgcatgccgc gagcagcggg ctgcccacgg cgaggccgag
     2341 aagaccgaac ccggctccct ggaggaactg gcgccgcgac ggccccggga gcgacatcgc
     2401 gcgagccgac atttcagaac ccgacatttc agaacccgat atttcagagc caggctcgtt
     2461 ctgcacgtga gacctccggg tttcgtgggc gacgagcccg aagtttcccg acgacctgta
     2521 ccagcgggca acaccgtgtt ctaatactcg gcacacgtta ccaagttgaa atgtttgggg
     2581 tggtccggat cgtcgccact agcggttctg cccgctcgac ccgccccgca atcgcgagcg
     2641 agtactccca gtcgctcgag cgcgggctcg cgatcttgtc ggccttcagc gagacccggc
     2701 ccctcctcgg caccgccgag ctcggccggt gcgtcgagtt gaacaagtcg accgcgcacc
     2761 gttacatctc gacgctgacc gagcttgggt atctccagca agaccagggc acccgcaagt
     2821 accgactcgg cccgcgggtg gtcaatctcg gcttcgcggc gatcaactcg atggagatca
     2881 ccaagatcgc ggcgcctcac atgcaggcgc tttcagacga gaccgggctg gccgtgagca
     2941 tggcggtgct cgatggcgtc gacatcgtgt acacccagcg ggtgcgtagt aacagcgccg
     3001 gtttcaccgg gatcgacctc aacctgcaca tcggttcgcg gctgccggcc tactgcacct
     3061 cgatgggcaa ggtgctgctg gcccaccagc gtcccgaggt gctgaaaagc atcctcgacc
     3121 gcaccgacct cgcccgccga ggccccaaca ccttgaccgc gcgcgaggcg ctcatcgccg
     3181 cattgcgccg ggtgcgccag atcggcctcg ccgtcaacga ccaggagctc gcccccggcc
     3241 tgcgctcact cgccgtcccc attcgtgatg ggttcggcga ggtcatcgcc gcgctgaaca
     3301 tcgcggtgca catgtcgatc ggcaacgcat cgctcgaatc gaccgtcagc cggctcgagg
     3361 gcccgctgcg caaggcggcc ggagagatat cgagccgcat gggatatcgc gaatcccccg
     3421 cggtgtaccg atagttagca cgctgttgta tttgacggaa cccccgatct atcttttgcc
     3481 aggttcgcga gatggaagag ggcgcacacg atgaccgagc agccgctgct ggcgcaggac
     3541 atctggcgag gcaagatctt cgtcgggggt agctgggcgc cgagcgcgcg gggcgcgacg
     3601 gctgcggtaa tagagcccgc gaccggcgca gaactcgggt cgatcggtga agccggagcc
     3661 gacgacgtcg ccgtcgctgc cgagtcggcg gccgcagcgc aggtcgcctg ggccgctacg
     3721 ccatacccgg ctcgggccgc ggttctgcgt cgcgcgggtg acctgtgggc cgagcacgcc
     3781 gaagagatca gctggtggaa catccgcgag gtcggcacga tccccggcat ggcggggttc
     3841 gccctccacg tcgcctcgca ggagtgctac gaagcgtccg cactgccgag tcgcgcctac
     3901 ggcgagctga tcccgaccga agagccgcga ctgtcgatgg ctcgccgcgt tccggccggc
     3961 gtggtcggag tcatctctcc gttcaacgtg ccgatcatcc tgggcatccg ttcggtcgcg
     4021 cccgcgcttg ccctcggcaa cgccgtgatc ctcaagcccg acccgcgcac gtccgtcacg
     4081 ggtggcgtga cgatcgcgcg catcttcgaa gaagcgggct tgccggccgg tgtcttgcag
     4141 atggtgcccg gcggtgtcga agtcggcgaa gcgctcgtga cccacccact cgtgcgggtg
     4201 atctcgttca ccgggtcgac gtcggctggg cggcgggtcg gcgagctggc agcgcgccac
     4261 ctcaagcggg tgcacctcga actcggcggc aactcggcga tgatcgttct cgatgatgcg
     4321 gacgtcgatg cagcggtcgg tctcgccgcc tgggggtcgt tcttccatca aggtcagatc
     4381 tgcatgacga ccggccgcca catcgtggcg gcaagccttt acgacgattt cgtcgagcgg
     4441 ttggcagcca gagctcagaa gcttcccgtc gggaacccgg caaccgatca ggtggcgctg
     4501 ggcccggtga tcgacgcgag gcaacgcgac aagattcaca gcttggtgac gcagagccaa
     4561 tcggcgggag cgagggtcgc cgccggtggc acgttcgagg gattgtt
//
LOCUS       DASWVF010000562         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_219565, whole genome shotgun sequence.
ACCESSION   DASWVF010000562 DASWVF010000000
VERSION     DASWVF010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219565"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..904
                     /gene="dapB"
                     /locus_tag="VGD55_09960"
     CDS             152..904
                     /gene="dapB"
                     /locus_tag="VGD55_09960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY0870710.1"
                     /translation="MSDLLKVVVLGANGRMGATVCDAVRAAPDLELVGGFDIGYSHDA
                     LVESGAEVVVDFTTPSAVMGNVEFCISHGLHVVVGTTGFDADRIATLRKWVDAYPGTG
                     VLIAPNFGIGAVLMMAFAEKAARYFESAEIVELHHPHKADAPSGTSRHTAERISAAWR
                     DAGLAPIPDATTSGLDGARGADVDGVHVHSVRLGGLVAHQEVLFGGHGETLTVRHDSY
                     DRVSFMPGVLLAVRNIAAHPGLTVGLENLLDI"
     gene            917..1495
                     /locus_tag="VGD55_09965"
     CDS             917..1495
                     /locus_tag="VGD55_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870711.1"
                     /translation="MTSESDPIDAEHANPEPVGSLQLDDVVLPIYELGAFNDADLRRV
                     KSFVTNFGSWLLPAGVASAVASHDGLGDLEWIHDTGELVLLGGVPTEGTISAELPGFA
                     GVSAELTPAFLGGEAGATEGDGPGMVREYFKSDVLPPGSRVALMAHIAHGPAAHELLW
                     GWHRQHRKEGGWLWLVERLARIESQDGSDHLG"
     gene            1499..3067
                     /locus_tag="VGD55_09970"
     CDS             1499..3067
                     /locus_tag="VGD55_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0870712.1"
                     /translation="MPALPERADVVIVGAGHNGLVAATFLARSGLDVVVVEAGTTIGG
                     AAKTERPFARVPDLQQSTGAYLLGLMPPELPRKLGVEFPVLRRDPHYFLPTTGDRYLL
                     FGSDRAATRRQYEEFFSAEDWKADEALQRELDLLRTDLAPAWLAEPMPLHETAQRYVR
                     PELQATFVDLCTGSVGDYLDRFGFTSDLVKAMYAVTDGLSGLDATWDTPGSGHNFLVH
                     NMCRLPGADGTWMVVRGGMGTVTSTLGQLALAAGATIVTGHAVTSIEISDAAVRGVVL
                     EGDRTVAAENVVVATDPFRLRHLVGSSVLPGALNAKLDSWKHYGTTMKVNLALTDLPT
                     FTCLPERRGQHGATIHLLPEDDVLGSLQRTYADVKAGRLADVPTIEWYIHTTVDPSLQ
                     DAGGHHSSALFVQWAPYEIAGSNWDAERAGYVEKLLSICDRFAPGTSELVADTFALAP
                     PDIEAHFGMTRGHIHHVDNAVAFADRMPYFTGVSGLYAGGAGCHPAGSVIGAAGHNVA
                     MRVLADLGRALPDL"
     gene            complement(3195..3761)
                     /locus_tag="VGD55_09975"
     CDS             complement(3195..3761)
                     /locus_tag="VGD55_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870713.1"
                     /translation="MSTPMHPHADGSVRVAVPPDASTIAGIQADALRQNYAALLPDEA
                     IDDFDVAAATNGWTEAITSPPSPRHRVFVAVGLDGVVGFAATAPATDTDLNPEADAEV
                     LALHVAPDQVRLGHGSRLMAAAVDHAHDDGVARLVMWVFAADDPLRMFLRENGWEADG
                     STRDLDVGELLHQVRMHTGIKDPPDLIA"
     gene            4121..>4524
                     /gene="thyX"
                     /locus_tag="VGD55_09980"
     CDS             4121..>4524
                     /gene="thyX"
                     /locus_tag="VGD55_09980"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY0870714.1"
                     /translation="MSEPSAGASERAEIAESAEAAEPVRPRFRSDVTVELIKHVASDA
                     DVLFAARVSTKGESSLDDVHADPSRSQGLLNYLMRDRHGTPFEHSSMTFYVQAPIFVF
                     REFMRHRTFSYNEESGRYRQLEPVFYTPAPER"
BASE COUNT          704 a         1491 c         1535 g          794 t
ORIGIN      
        1 tgagcaggtg cgcgaggtcg ccgcttcagt cttgtcggcg aagcagacgc ttgcggtgat
       61 cgggcccttc gacgacggtg cgtttgcgga cgctgtcgcg tgagcgactg gatgcggggc
      121 gtcgcgtgag cgactgggtg cggggcgtcg cgtgagcgat ctcctgaagg tcgtagtcct
      181 cggggcaaac gggcgcatgg gcgcgaccgt gtgcgacgct gtgcgcgcag ccccagatct
      241 tgagttggtc ggcgggttcg acatcggcta cagccacgac gccctcgtcg agtcaggcgc
      301 agaggtcgtc gtcgacttca cgaccccatc tgcggtgatg ggcaatgtcg agttttgcat
      361 tagccacggc ctgcacgtcg tcgtcggcac tacaggcttc gatgccgacc gcatcgcgac
      421 gctacgaaag tgggtcgacg catatcccgg gaccggcgtg ctgattgcgc ccaacttcgg
      481 gatcggcgcc gtgctcatga tggcgttcgc cgagaaggct gcccgctatt tcgagtcggc
      541 cgagatcgtc gagctgcatc acccgcacaa ggccgacgcc ccttctggca cttccaggca
      601 caccgctgag cgcatttcgg ccgcatggcg cgacgctggc cttgcgccga tccccgacgc
      661 cacgacgtcc ggccttgatg gtgctcgcgg ggctgacgtc gacggcgtgc acgttcattc
      721 ggtgcgcctc ggcggtctgg tggcccacca ggaggtgctg ttcggcgggc atggtgagac
      781 gctgacggtg cgccacgatt cctacgaccg ggtgtcattc atgccgggtg ttttgctcgc
      841 ggtgcgtaac atcgcagcgc acccaggtct gacagttgga cttgagaacc tgctcgacat
      901 ctagagaggc agcccgatga cgtccgagtc cgaccccatt gatgccgagc acgcgaaccc
      961 cgagccggtc ggatcgctcc agctcgacga cgtcgtgctg ccgatctacg agctcggcgc
     1021 gttcaacgac gccgatctgc gtcgggtgaa gagcttcgtg acgaacttcg ggtcatggct
     1081 gttgccagct ggcgtggcga gtgccgtggc ctcgcatgac ggtcttggcg atctggagtg
     1141 gattcatgac accggcgaac tcgtgctgct cgggggcgtc ccgaccgaag gcaccatctc
     1201 cgctgagctg cccgggttcg cgggcgtgtc tgccgaactg acgcccgcat ttctcggcgg
     1261 agaggccggg gcgaccgagg gcgatgggcc ggggatggtg cgcgagtact tcaagtccga
     1321 cgtgctcccg cccggatctc gggttgcgct gatggcccac atagctcacg gacctgcagc
     1381 gcatgagttg ttgtggggct ggcaccgcca gcaccgcaaa gagggcggct ggctatggct
     1441 ggtcgagcgc ctcgcgcgga tcgagtcgca agacggctct gaccacctcg gctgatcggt
     1501 gcccgcgctg cccgaacggg ctgacgttgt catcgtcggc gccggccaca acggtcttgt
     1561 cgcggcgacg tttctcgccc gcagcggtct cgacgtcgtt gtcgtcgaag cagggacgac
     1621 gattggcggc gcggccaaga ccgagcggcc gttcgcacgg gtgcccgacc tccagcaatc
     1681 gaccggcgcc tacctgctcg ggttgatgcc gcccgaactt ccccgcaagc tcggcgtcga
     1741 gtttccggtg ttgcggcgcg acccgcacta cttcttgccg accaccggcg accggtacct
     1801 gttgttcggc tccgaccgcg ccgcaacgcg tcggcagtac gaggagttct tctcggccga
     1861 agactggaag gccgacgagg cgctgcaacg cgaactcgac ctgctgcgta cggatctcgc
     1921 ccccgcctgg ctcgccgagc cgatgccgct gcacgagacc gcgcagcgct acgtccgccc
     1981 cgagttgcag gcgacgttcg tcgatctgtg cacgggcagt gtcggtgact atctcgaccg
     2041 tttcggcttc accagcgacc ttgtgaaggc gatgtacgcc gtcaccgacg gcctgtcggg
     2101 cctcgatgcg acctgggaca ccccaggctc cggccacaac ttcctcgtac acaacatgtg
     2161 ccgcctgcca ggcgccgacg gcacctggat ggttgtgcgc ggcggcatgg gcaccgtgac
     2221 gtcgactctc ggtcagctag cgctcgcggc cggtgcgacc atcgtcaccg gccacgccgt
     2281 gacaagcatc gaaatatcgg acgccgcggt gcgtggcgtc gtcctcgaag gtgaccgcac
     2341 tgttgctgct gagaacgtcg tggtggcaac ggatccgttt cggttgcggc acctcgtggg
     2401 ctcctctgta ttgccggggg ccttgaacgc caagctcgat tcgtggaagc actacggcac
     2461 cacgatgaag gtcaatcttg cgctcaccga tctgccgacg tttacgtgct tgccggagcg
     2521 ccgcggccag cacggcgcga cgatccatct gctgcccgaa gacgacgtgc tcggctcgct
     2581 tcagcgcacg tatgccgacg tgaaggcggg tcggttggcc gacgtcccga ccatcgagtg
     2641 gtacatccac acgacggtcg acccttcgtt gcaggacgcc ggcggccacc actcgtctgc
     2701 attgttcgtg caatgggcgc cgtacgagat cgccggttct aactgggacg ccgagcgtgc
     2761 cgggtacgtc gagaagttgc tgtccatctg cgaccgcttt gcgccgggca catctgagtt
     2821 agtggccgac acgttcgcgc tggcgccacc cgacatcgaa gcccacttcg gcatgacccg
     2881 cggacacatt caccacgtcg acaacgccgt cgcgttcgcc gaccggatgc cttatttcac
     2941 aggcgtttca gggctgtacg ccgggggagc ggggtgccac ccagccggct cggttatcgg
     3001 tgccgcgggc cacaacgtcg cgatgcgggt gctagccgac ctaggccgcg cgttgcctga
     3061 cctctaacga ccacctctca cccgcaacag tggcggccgg gcccggcaag ttggaagccc
     3121 ctggacactc aagagagcgt tctcgggctt ccaagttggc acgaagcgcg gtcggagggt
     3181 gggtggggca gggtttaggc gatgaggtcg ggcggatcct tgatcccggt gtgcatccgc
     3241 acctggtgca gtagttcgcc gacatcgaga tcgcgggtcg agccgtcggc ttcccagccg
     3301 ttctcgcgca ggaacatccg cagtgggtcg tcggccgcga agacccacat gaccaggcgg
     3361 gcgaccccgt cgtcgtgcgc gtggtcgacg gcggccgcca tcaaccggct gccgtgcccc
     3421 agccgcactt ggtctggagc gacgtgcaac gccagcacct cggcgtccgc ctcaggattg
     3481 aggtctgtgt ctgtcgcggg ggccgtcgct gcgaagccca ccacaccgtc gagtccaacc
     3541 gcgacgaaca cccggtgccg gggggacggc gggctggtga tggcctcggt ccagccgttg
     3601 gtcgcggccg cgacgtcgaa gtcgtcgatc gcctcgtcgg ggagcagcgc cgcgtagttc
     3661 tgccgcaggg cgtcggcttg aatgcccgcg attgtcgacg catcaggcgg gacggcgacc
     3721 cgcacgctgc catcggcgtg cgggtgcatg ggggtcgaca ccgcgccatc ttcgcagcag
     3781 ccacgcgcca tcttcgcagc aggcaaaagt gtcagtggcc gcggatagaa aggaccggtc
     3841 ggcggagcac tgaagccggg ctcgaggccc aagaggagat ggcgatgtac ggatatctga
     3901 accagttggt agcagaggcc cacatcaacg aattgcgggc ccacgcagcc cgtcagcgac
     3961 tgattgccca ggcagcgaag gcagcccgag ggtctcgcgg ccgcaagtct ggggttatcg
     4021 cggcatcggc cgctccggtc ggcccggttt cggcgggcgt tacccgaacg gcgagcctcc
     4081 gggtcaggcc ggctgcggac tgctgactat ggtgtccgtc atgtccgagc catccgctgg
     4141 agcctctgag cgggctgaga tcgcggagtc ggccgaggcc gccgaacccg tgcggccgcg
     4201 ctttcgcagc gacgtcacgg tcgagctgat caagcacgtg gcgagtgacg ccgacgtgct
     4261 cttcgccgcc agggtctcga cgaagggcga atcgtcgctc gacgatgtcc acgccgaccc
     4321 ttcgcgttcg cagggcctgc tcaactacct catgcgcgac cggcacggca cgccgttcga
     4381 gcactcgtcg atgacgttct acgtgcaggc cccgatcttc gtcttccgtg agttcatgcg
     4441 gcaccgcacc ttcagctaca acgaggagag cggccgctac cggcagcttg agccggtctt
     4501 ctacaccccg gcgcccgagc gcaa
//
LOCUS       DASWVF010000563         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_244676, whole genome shotgun sequence.
ACCESSION   DASWVF010000563 DASWVF010000000
VERSION     DASWVF010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244676"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VGD55_09985"
     CDS             <1..973
                     /locus_tag="VGD55_09985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEY0870715.1"
                     /translation="PGVDVSDLAASLAGAAAHRTLNAGPNHVLTAAPNWMPGPADDPT
                     PADAVPPPHFPSTTPARPVTVAILDTGISAHPWFTGQTWFGECGPEVQEVPDADHDSR
                     LDSVAGHGTFIAGVVAKHAPAASLMIGKIIAGDGVTDELRLLRGLSRLRAHAHEHRTP
                     VDVISLSLGCYTHDDKPSPVLEHGLNSFGHETVVVACAGNFGSHRPYWPAAMKRVVAV
                     ASLDAKGVDRAEFSNYGWWVDASTVGENVVSSFFDFDGPVGSSPTPEDFTGYATWSGT
                     SFAAPRVAAAIAARAGTHGVPAAAAAAELLDPTEHRSMPDLGVLVDP"
     gene            1032..1616
                     /locus_tag="VGD55_09990"
     CDS             1032..1616
                     /locus_tag="VGD55_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0870716.1"
                     /translation="MLELSTSELLAEAGAGNSAAWDELVRRYSGLVWAVARGFALSMA
                     DAADVSQTTWLRLVEHLTKLREPEHLGGWLATTARNECLRLLRKNGREVIDADVGVDL
                     ESGEPTPEAVVLDNERDRLLWLSLGEIPQRCQVLLRALALTPPPSYGEVSAALGMPVG
                     SIGPTRARCLDHLKKRMLVHQSAAGTATQGEGGE"
     gene            1616..2068
                     /locus_tag="VGD55_09995"
     CDS             1616..2068
                     /locus_tag="VGD55_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870717.1"
                     /translation="MVEPTQDPLLTSLFNVVTNVDPVPASVVEAARAAFIWRTIDAEL
                     AELTADSAMATAGVRSSSAPRLLTFEGAGVEVEVEVAQTGSTRRLTGQLVPVGPARVT
                     VRWSGGTQEAVADDLGRFAVEGIPAGTVSLVVDRAGAEHPIVTSWISI"
     gene            2082..4700
                     /locus_tag="VGD55_10000"
     CDS             2082..4700
                     /locus_tag="VGD55_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0870718.1"
                     /translation="MSVTTAPELTRLQQRAEAVYGQVSVAPALAETSARRIIEESHSD
                     PTARVMAMRALALTVRQTAGPIESIKILREAVRFGERHRLGRCVAEARMTYAAMLADT
                     GRIPAALTECDRAATILKGRDAGPLLAQRALILARAGRSEEAIANFARALPLLRAAGD
                     VRFQCLLFINRSNLLAYLGRLGAAERDLRGGIELARAHGLHDLVTVMSENLGFLKLRR
                     GDIPAALRLFAVALEGADTAPGLVATHDRAEALLIAGMPGEARESLERRVDDVEEAGF
                     AVDLAEWHLLLARAALMEGEADIARAAAQRALAEFRAQGRARWALLAQQIVIRARWAS
                     GERTTALSRAARDAHAKLWTAGWQVAALHCLVVAGRVELEAGRLSSARADLARAAQAR
                     RRGPADLRAAAWYAEAMLRLASEDTRGATTALKAGLRVVDDHAASLGATDLRVHATGL
                     GADLAEQGVRLAVESGKPVAVLEWIERFRAGTLRRRPVRPPSDARLAGELADLRRVTA
                     ELAQTTADGEDSRGLRAEQLRLEEAVRHRSRHARGSRAPIREFDVDELRAAVGERALV
                     EILRVDDAMLAVTLTDDRLRLHELGSYPEAMRELESLRFSLHRLARKHGSATSLAAAQ
                     VGLSHATDSLDASLMRPVLDVVGDRELILVPTGRLHALPWPTLPSLRGRPVCVAPSAT
                     TWLAATRANSRSRPGSRVVLVAGPNLEHAEPEVAAVAKLYNSPAVLSGPGATADVVRQ
                     KMDGAQLVHIAAHGRFRADNPQFSAIDLADGPLTVYDLERVRRGPRQLVLSACDSGLS
                     AVHAGDELMGLAGAVFSLGTSTLIASVVPVADDLTEALMVEVHRELCAGNSASHALAI
                     AQQRVRADGFVCFGAA"
     gene            4700..>5146
                     /locus_tag="VGD55_10005"
     CDS             4700..>5146
                     /locus_tag="VGD55_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870719.1"
                     /translation="MADSIVVATTPSEYDAFGVLIREYWDWLLTRYSVHPGLMEDIGA
                     HQGLDDELNTVATVYRPPAGKALLAMRHGQVSGGVGYRDLHDGSCEMKRMFVPERFQG
                     HGTGRLLCEALMTTAADDGFRLMRLDTGYLNAEAMGMYASLGFSPCP"
BASE COUNT          805 a         1713 c         1759 g          869 t
ORIGIN      
        1 acccggcgtc gacgtgagtg acctcgccgc aagcctcgcc ggcgcggcag ctcaccgcac
       61 gctcaacgcc ggcccgaacc atgtgctcac ggcggcgccg aactggatgc ctgggccagc
      121 cgacgaccca acgcccgccg acgccgtccc gccgccgcat ttcccatcga caacgccagc
      181 gcgccccgtc acagtcgcga tcctcgacac cggcatctcg gcgcacccgt ggttcacggg
      241 ccagacttgg ttcggcgaat gcgggcccga ggtgcaggag gttcccgacg ccgaccatga
      301 cagtcggctc gactccgtcg caggtcacgg cacgttcatc gccggcgtcg tcgcaaagca
      361 cgccccagca gcaagtctga tgatcggcaa gataatcgcg ggtgacggcg ttaccgacga
      421 actccgactg ctgcgcgggc tttcccggct gcgcgcgcac gcacacgaac accggacgcc
      481 ggtcgatgtc atcagcttgt cgctcggctg ttacacccac gacgacaaac cttcacctgt
      541 cctcgagcac gggctcaact cgttcgggca cgagactgtg gttgtcgcgt gcgcggggaa
      601 cttcggcagc caccgtccgt attggccagc tgccatgaag cgggtcgtcg ccgtcgcctc
      661 gctcgacgcg aaaggtgtcg accgcgcgga gttctcgaac tacggctggt gggtagacgc
      721 ctccaccgtc ggggagaacg tcgtgtcgag cttcttcgat ttcgatggtc cggttggcag
      781 ctcccccacc ccggaagact tcacgggcta cgcgacgtgg agcggtacct ccttcgccgc
      841 acccagggtt gcggccgcca tcgcggcgcg cgcggggacc catggcgttc cggcggccgc
      901 ggcggccgcc gagcttctcg acccgaccga gcaccgctcg atgcccgatc tcggcgtgct
      961 ggtcgaccca tgagggatat cgtcgtgatc ctgcgtaacc ggagaccaaa gtgcctcggg
     1021 agggtcctgc cgtgctcgag ctcagcacga gcgagcttct tgcagaggcg ggggcgggca
     1081 acagcgcggc gtgggacgag cttgtccgcc gctactcggg tctggtctgg gcggtcgccc
     1141 gcggattcgc actctccatg gcagatgccg ccgacgtcag ccagacgacc tggcttcggc
     1201 ttgtcgagca cctcacgaag ctgcgtgaac ccgaacacct cggtggctgg ctcgccacca
     1261 ccgcacgcaa cgaatgcctg cggctgctgc gaaagaacgg ccgcgaagtc atcgatgccg
     1321 acgtcggcgt cgacctcgaa tcgggcgaac ccacccctga ggcggtcgtg ctcgacaacg
     1381 agcgcgaccg attgttgtgg ttgtcacttg gcgagatccc gcaacggtgt caggtcttgc
     1441 tgcgtgcgct cgcgctcacg ccgcccccaa gctatggcga ggtttccgca gctctcggga
     1501 tgccggtggg cagcatcggc ccgacgcgag cacgctgcct tgaccatctc aagaagcgga
     1561 tgctcgtgca ccagagcgct gccggcactg ctactcaagg cgaagggggc gagtgatggt
     1621 cgagccgact caagacccgc tactgacctc gctgtttaac gtggtcacga acgtcgaccc
     1681 ggtgccagct tcggtcgtcg aagcggctcg ggccgcgttc atatggcgaa cgatcgacgc
     1741 cgaactggcc gaactcaccg ccgactcggc gatggcgacg gcgggtgtgc ggtcgtcgtc
     1801 cgccccgagg cttttgacct ttgaaggcgc cggggtcgag gttgaagtcg aggtcgccca
     1861 aaccggttca acgcggcgcc tcaccggtca gctggtgccg gtcggccctg cgcgcgtgac
     1921 cgtgcgctgg tccggtggca cccaagaggc agtggccgac gacctcggca ggttcgcggt
     1981 tgaagggatc ccggctggca ctgtgagcct tgtggtcgat cgcgccggcg ccgagcaccc
     2041 gattgtgacg agctggatct cgatctgaca cgctgcccag catgtcggtc accactgccc
     2101 cggagctgac gcggcttcag cagcgtgccg aggcggtgta cggccaggtg tcggtcgcgc
     2161 cggcactcgc ggagacgagc gcccgccgca tcatcgagga gtcgcactcc gacccgactg
     2221 cgcgggtcat ggcgatgcgt gcgctggccc tgaccgttcg gcagacagcc ggacccatcg
     2281 agtcgatcaa gatcctgcgc gaagctgtcc gtttcggtga gcggcatcgc cttggccgat
     2341 gtgtcgctga ggcgcggatg acctacgcgg ccatgctcgc cgacaccggt cgaatacctg
     2401 cggccctcac cgaatgcgat cgcgccgcga cgatcctgaa gggaagggac gccggtccgc
     2461 tgctcgccca acgcgcgctg atcctcgcgc gggcgggacg ttccgaagag gcgatagcga
     2521 acttcgcccg cgcgttgccg ctactacggg cggcaggcga cgtccgattt caatgcctgc
     2581 tcttcatcaa ccgcagcaac ctgctcgcct atctcggacg gctgggtgcc gccgagcgcg
     2641 atcttcgcgg tggcatcgag ctagcacgcg cgcacgggct gcatgacctc gttacggtca
     2701 tgtcggagaa cctcggcttt ctcaagttgc ggcgcggcga tattccggcc gcgcttcgac
     2761 tgtttgcggt ggcactcgag ggagctgaca cggcccccgg gctcgtcgcc acgcatgacc
     2821 gcgcggaagc gctgttaatt gcgggcatgc caggtgaggc gcgggagtcg ctcgagcgac
     2881 gggtcgacga cgtggaggag gccggattcg cggtcgatct cgccgagtgg cacctcttgc
     2941 tggcacgcgc ggcgttgatg gagggcgaag ccgacatcgc gcgagcagcg gcgcaacgcg
     3001 cgctcgccga gttcagagcc cagggtcgcg cccgctgggc gttgctcgcg caacagatag
     3061 tgattcgtgc gcggtgggcg agcggtgaac gaacgactgc gctgtctcgc gcggcccgag
     3121 atgcacacgc aaaattgtgg acggcggggt ggcaggtcgc ggcgctgcat tgcctcgttg
     3181 tcgccggccg tgtcgagctt gaggctggcc gattgtcatc agcgcgcgcc gacttggctc
     3241 gggcagcgca agcgcgccgg cgcgggcccg ccgatctgcg cgctgcagcc tggtacgccg
     3301 aggccatgct gcggctggca tcggaggaca cccgcggcgc gaccaccgcg ctgaaggcgg
     3361 gcctgcgggt ggtcgacgac cacgcggcat cgctgggtgc gaccgacctg cgagtgcacg
     3421 caaccgggct tggcgccgat ctcgccgagc agggcgttcg gctggcggtc gaatccggca
     3481 agccggtcgc cgtcctcgaa tggattgaac ggtttcgggc cggcaccctg cggcgtcgac
     3541 cggtgcggcc accctcagat gcccggctcg caggtgaact cgcagacttg cgtcgggtca
     3601 ccgccgagtt ggcgcagacg acggccgacg gcgaagactc gcgcgggttg cgcgccgagc
     3661 agctgcgtct cgaggaagcc gtgcggcacc ggtcgaggca cgcgcgaggc agccgcgctc
     3721 cgatccgcga gttcgacgtc gacgagttgc gcgccgcggt aggtgagcgc gcgttagtcg
     3781 agatcctgcg tgtcgacgac gcgatgctcg cggtcacgtt gaccgacgac cgattgcgcc
     3841 tgcacgaatt gggctcgtat cccgaggcga tgcgcgagct cgagtcgctg cggttctcgt
     3901 tacaccggct agccagaaag cacggatcgg ccacgtcgtt agcggccgcg caggtaggac
     3961 tgtcccacgc cacagattcc ctcgacgcct cactgatgcg accggtgctc gatgtcgtcg
     4021 gtgaccgcga gttgatcctc gtgccgaccg gtcgactcca cgcgctgccg tggccgacgc
     4081 tgccgtcatt gcgcggccga ccggtatgcg tcgcgccgtc ggcgacgacg tggctcgcag
     4141 caacgcgcgc gaacagtcgg tcgcgccctg gcagccgtgt tgttctcgtc gccgggccca
     4201 atctcgaaca cgcggagcct gaggtcgcgg ctgtcgcgaa gctgtacaac tcacccgcgg
     4261 tgctgagtgg tcccggtgcg acggcggacg tcgtcaggca gaagatggac ggcgcgcagc
     4321 tggtgcacat cgccgctcac ggtcgcttcc gcgcagacaa cccgcagttc tcggcgatcg
     4381 atctcgcaga tggtccactc accgtctatg acctcgaacg cgtgcggcgc ggaccacgac
     4441 aactggtgtt gtcggcgtgc gattcgggac tgtctgcggt gcacgcgggt gacgaactca
     4501 tgggtctggc cggagctgtt ttttctttag gtaccagtac attgatcgcg tcggtcgtgc
     4561 cagtagcgga cgatctcacc gaggccctca tggtcgaggt gcaccgcgag ttgtgcgccg
     4621 gcaattcggc gtcccacgca ttggcgattg cacaacaacg cgtacgcgcc gacggcttcg
     4681 tctgcttcgg cgccgcctga tggctgactc gatcgtcgtc gcgactacgc ccagcgagta
     4741 tgacgcgttc ggcgtcttga tccgcgagta ctgggactgg ttgctgacgc ggtactcggt
     4801 tcacccgggc ctcatggaag acatcggagc tcatcaaggt ttggacgatg aactgaacac
     4861 agtcgccacc gtgtaccgac cccccgcagg taaggcgctg ctcgccatgc gtcacggcca
     4921 ggtcagtggc ggagttggct atcgagattt gcatgacggc tcgtgtgaga tgaagcggat
     4981 gttcgtccct gagcgtttcc aggggcacgg caccgggcgc ctactgtgcg aggctttgat
     5041 gacgacagca gccgacgacg gcttccggct gatgcgactc gacacgggct atctcaacgc
     5101 tgaggcgatg ggcatgtatg cgtcgctggg cttctcgcca tgcccg
//
LOCUS       DASWVF010000564         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_65998, whole genome shotgun sequence.
ACCESSION   DASWVF010000564 DASWVF010000000
VERSION     DASWVF010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65998"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..758
                     /locus_tag="VGD55_10010"
     CDS             138..758
                     /locus_tag="VGD55_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870720.1"
                     /translation="MTRSHITVRAAGEPDVAALVVLWEELKRYGGLNPRVAALTDDRK
                     IEERVRRLISRPTSRILLASIADEPVGMAMLTTVPLGALNETACVQVEYTVVAEGYRR
                     RGVGRALMGAAACYAEDIGADQVTVSVSPMSREANRFFAQLGLTPMSVRRVAPASALR
                     RRIATLEHTPNPERATRRPLGTRTAVRTALRRLALAAPVADQPHDA"
     gene            821..3526
                     /gene="polA"
                     /locus_tag="VGD55_10015"
     CDS             821..3526
                     /gene="polA"
                     /locus_tag="VGD55_10015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0870721.1"
                     /translation="MTPLEAARERPRLLLIDGHSVAYRAFYALPVQNFSTTTGQPTNA
                     VYGFTSMLINVLRDEKPSHLAVAFDISRQSFRTETFAEYKANRSATPNEFKGQISLIQ
                     DVLTALAIPVVTAEGYEADDVIATLATQAEDAGLDVLICSGDRDVFQLVSDHVTVLYP
                     RKGVSDMARMTPEAVEERYGLTPAQYADFAALRGDPSDNLPSIPGVGEKTAAKWVHDY
                     GSLTGLVDRVDEVPGKAGGLLREHLASVLRNRQLTELVREVPLSVGPLDLQLGQWNRE
                     EVHRIFDSLQFKVLRERLYATLSAPEPEADAGFTVEGGLLGPGEVPAWLSEHTAPGVR
                     VGVSVAGSWGRGTGIVTGLALAAADGAGAYLDPTQLTAADDQALAAWLADPDRPKAVH
                     DAKGPTLALAAHGWPLAGLSSDTALAAYLALPGQRSFDLGDLALRYLHRELRADGATA
                     ANGSAQLSLDGLDEDGAAVNEIVRARAVLDLATALDVDLDERGATKLLTDVELPLIGV
                     LAGLEQVGIATDLEVLADLSSELGAAVKVAEQAAFAVVGREFNLGSPKQLQQILFDEL
                     GLPKTKRIKTGYTTDADALQSLLAQTEHPLLEHLLHHRDVSRLKTVVDSLIPMIDDHG
                     RVHTTFNQLVAATGRLSSTDPNLQNIPIRTAEGRRIRQAFVVGAGFESLMTADYSQIE
                     MRIMADLSGDDALVEAFSSGFDFHATTAASVFGVPLVDVDGVLRNRVKAMNYGLAYGL
                     SAYGLSQQLGISADEARGLMVEYFERFGGVRDYLSRVVAQARQDGYTETILGRRRYLP
                     DLNSDNRQRREMAERMALNAPIQGSAADIIKVAMLNVDRALRAAGLKSRMLLQVHDEL
                     VFELAPGERADVEVLVRREMAGAYPLKAPLDVSVGVGPNWDAAGH"
     gene            3747..>4351
                     /locus_tag="VGD55_10020"
     CDS             3747..>4351
                     /locus_tag="VGD55_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870722.1"
                     /translation="MSTRSFQRLGVAGLAALTVVGLLSGCAAKKKTASASTAAASTAA
                     AATDTSSKVVATGGGKFCQQVADSVNNNVAKEAATGGSSDSLKTSVQNFKSIEGSVLK
                     SAPGAIKPDLVTLFGALDQFYSALAADNYDFTKIDPSVEAPLETPAVQAAEKNVDDYM
                     KNTCGIDIGGDAASANTGDVEASASAALASALAKLTASAAPA"
BASE COUNT          758 a         1458 c         1407 g          728 t
ORIGIN      
        1 gtctccacat cgccgccaaa cctcgctacg ctccggtttc tggcacggtt cgggaacgcg
       61 ttcgcgagag ccgggggcaa tgagcttgat gcggcgcgtg gcgtcgtgga cgagcacgtc
      121 gttcttagga gcaaagcgtg acccgatctc acatcacggt gcgagccgcc ggcgaacccg
      181 acgtggctgc cctcgtcgtc ctgtgggaag agttgaagcg ctacggaggt ctcaatcccc
      241 gcgtggctgc gctcaccgat gaccgcaaga tcgaagagcg agtacgccgc ctgatcagcc
      301 gcccgaccag ccggatcctg ctcgccagca tcgccgacga gcccgtcggg atggccatgc
      361 tgaccactgt tccactcggc gcgctgaacg agacggcgtg cgtgcaggtc gagtacacgg
      421 tcgttgcaga gggatatcgc aggagggggg tcggccgcgc tttgatgggc gccgctgcct
      481 gctacgccga agacatcgga gccgaccagg tgaccgtgag cgtctcgccg atgtctcgcg
      541 aggcaaatcg cttctttgca caactcggtc tgacgccgat gtcggtacgc cgcgtcgctc
      601 cggcctcggc cttgcggcgc cgcatcgcga cgctcgagca cacccccaat cctgagcgcg
      661 ccacgaggcg cccacttggc acccgaactg ccgtgcgcac cgcgctgcgg cggctcgccc
      721 ttgccgcgcc ggtcgccgat caaccgcacg acgcctgagc gctgccggcc gctagctctc
      781 ggcgccgcca ccgtcagacc cagtgcctag gatcacaggc gtgacccccc ttgaagctgc
      841 ccgcgagcgt cctcgactgt tgttgatcga cggccattcg gtggcctacc gagcgttcta
      901 cgcgctgccc gtgcagaact tttcgacgac tacaggtcaa ccgaccaacg ccgtgtacgg
      961 ctttacgtcg atgctcatca acgtgcttcg tgacgagaag ccgagccacc tcgccgtcgc
     1021 gttcgacatc tcacgacaga gttttcgcac cgagacgttc gccgagtaca aggcgaacag
     1081 gtcggcaacg ccgaacgagt tcaagggcca gatcagcctg atccaagacg tcctcaccgc
     1141 tctcgcgatt cccgtcgtca ctgctgaggg ctatgaagcc gacgacgtga tcgcgacgtt
     1201 ggcgacgcaa gccgaagatg cgggtctcga cgtgctcatc tgctcaggtg accgcgatgt
     1261 cttccagctc gttagcgacc atgtcacggt gctgtacccc cgcaagggcg tctccgacat
     1321 ggcgcgcatg acacccgagg ccgtcgaaga gcggtacggc ctgacgcccg cgcaatacgc
     1381 cgactttgct gcactacgtg gtgacccaag cgacaacctg ccgagcatcc ctggtgtcgg
     1441 tgagaagacc gcggcgaagt gggtgcacga ctacgggtcg ttgaccggtc tcgtcgatcg
     1501 cgtcgacgaa gttcccggca aagcaggcgg tctgctgcgc gaacacctgg cgagcgtgct
     1561 gcgaaaccgc cagctcaccg agctcgtgcg cgaagtgcca ctatccgtcg gtccgctcga
     1621 cttgcagctc ggccaatgga atcgcgagga agtccaccgc atcttcgact cgttgcagtt
     1681 caaggtgttg cgcgagcggc tctatgcgac cttgtcggcg cccgagcccg aggccgacgc
     1741 cgggttcacc gtcgagggcg gcttgcttgg tccgggcgaa gtgccggcat ggctcagcga
     1801 gcacaccgcg cccggggttc gggtcggtgt ttctgtcgcc ggtagctggg gccgtggcac
     1861 cggcatcgtc acggggctgg cattggctgc tgctgacggc gcgggcgcgt atctcgaccc
     1921 gacccaactc acggcggccg acgaccaggc actggcggcc tggctggctg acccagaccg
     1981 acccaaggcc gtgcacgatg cgaagggccc gacgctggca ctggctgcgc acgggtggcc
     2041 gcttgcgggc ctgagcagcg ataccgcgct cgcggcctac ctcgcgttgc ccggccagcg
     2101 ttccttcgat ctaggtgacc ttgcgctgcg atatctgcat cgcgaactgc gtgccgacgg
     2161 ggccaccgcc gcgaacgggt ctgcgcagtt gtcgctcgac ggtctcgatg aagacggcgc
     2221 ggcggtcaac gagatcgtgc gagctcgcgc ggtgctcgat ttggcaacag ccctcgacgt
     2281 cgacctcgat gaacgcggcg caacgaagct cctcaccgac gtcgaactgc cgctcatcgg
     2341 cgtactcgcg ggtctcgagc aggtgggcat cgccaccgat ctcgaggtgc tcgccgacct
     2401 gtcgagcgaa ctcggcgctg cggtcaaggt tgccgagcag gccgcgttcg cggtcgtcgg
     2461 ccgtgagttc aacctcggct cacccaagca actccagcag atcctgttcg acgagcttgg
     2521 cctgccgaaa acgaaacgca tcaagacggg ctacaccacc gacgccgatg cgttgcaaag
     2581 cttgcttgcc cagaccgagc atccgttgct cgagcacttg ttgcaccacc gcgacgtgtc
     2641 gcggttgaag accgtcgtcg actcgttgat tccgatgatc gacgatcacg gccgcgtgca
     2701 cacgacgttc aaccaactgg tcgccgcgac aggccgactg tcgtccaccg acccgaacct
     2761 ccagaacatt ccgatccgaa ccgccgaagg ccgccgcatc cggcaagcct tcgtggtcgg
     2821 ggcggggttc gagtcgctga tgacggccga ctacagccag atcgaaatgc gcatcatggc
     2881 cgacctgtcg ggtgacgacg ccctcgtcga ggcattcagc tccggattcg attttcacgc
     2941 aacgacggcc gcgagcgttt tcggcgtccc tcttgtcgat gtcgatggcg tcttgcgaaa
     3001 ccgggtgaag gcgatgaact acgggctcgc ctacggcctg tcggcatacg gcctgtcaca
     3061 gcagctcggc atcagcgccg acgaagcacg cggcctcatg gtcgagtact tcgaacggtt
     3121 cggcggtgtg cgcgattacc tcagccgcgt tgtcgcacag gcccgccagg acggctacac
     3181 cgagacgatt ctcgggcgcc gccggtacct gcccgatctc aacagcgaca acaggcaacg
     3241 tcgcgagatg gccgagcgca tggcgctgaa cgctccgatc cagggcagcg ccgccgacat
     3301 catcaaggtc gcgatgctca acgtcgaccg tgcattgcgc gccgcggggc tgaagtcccg
     3361 gatgctcctc caggttcacg acgagctggt cttcgagctc gcgccaggcg agagggcgga
     3421 cgtcgaggtg ctggtgcgtc gtgaaatggc cggcgcctac cccctcaagg caccgctcga
     3481 cgtttcggtc ggggtgggcc cgaactggga cgccgccggc cactgacccc gtccggctac
     3541 agggcgccca cgccacgccg tagcgaacgt tgcgatcttg tgctggtcgc gcgtggggcg
     3601 ccccggtatc ttcttgcccg ttacaacatc cgcagaccaa tcggggtggg cggatgtatc
     3661 aagcggcgga cctggggtct cctcacgatt ggtcggagaa ccgaccggtt gcaggcactt
     3721 cgatccacct acctggggag acgcacgtga gcacgagatc cttccaacgt cttggtgtcg
     3781 ctggtcttgc tgcgctcaca gtggtgggcc tgctgagcgg ctgcgcggcc aagaagaaga
     3841 ctgcgagcgc atcgaccgcg gcggcgtcga ccgcggcggc ggcgaccgac acgtcctcga
     3901 aggtcgtggc gaccggaggc ggcaagttct gccagcaggt tgccgacagc gtcaacaaca
     3961 acgtggccaa ggaggccgcg accgggggga gctccgactc gctcaagacg agcgttcaga
     4021 acttcaagag cattgagggc agcgtgctca agtcggcgcc gggcgcgatc aagcctgacc
     4081 tggtgaccct gttcggtgcg ctcgaccagt tctacagcgc gctcgcggcg gacaactacg
     4141 acttcaccaa gattgacccg agcgtcgagg cgccactgga gaccccggca gtacaagccg
     4201 cggagaagaa cgtcgatgac tacatgaaga acacctgcgg catcgacatc ggcggcgacg
     4261 ctgcgtccgc caacaccggt gacgttgaag cgtccgcgtc tgcagcgttg gcgtccgctc
     4321 tggcaaaact gactgcgtcg gcggctccgg c
//
LOCUS       DASWVF010000565         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_117444, whole genome shotgun sequence.
ACCESSION   DASWVF010000565 DASWVF010000000
VERSION     DASWVF010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117444"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /gene="rpoB"
                     /locus_tag="VGD55_10025"
     CDS             <1..2076
                     /gene="rpoB"
                     /locus_tag="VGD55_10025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0870723.1"
                     /translation="AKRDGIVESVDATRIVVRPTQESDDDPLSAKPDIYNLTKFQRSN
                     QNTCINQKPIIERGDPVKMGDVIADGPATERGELALGENILVAFMPWGGYNFEDSILV
                     SENLVKNDHFTSIHIEEFECVARDTKLGKEEITCDIPNVGEEALKDLDESGIVRIGAE
                     VKPQDILVGKITPKGETQLSPEEKLLRAIFGEKAGDVRDSSLRVPPGVQGIVISAKVF
                     SRKGTEKDERAKDIEDQEKEKLLADQRDEVKIVSESYHAKMRKLLVGKTTAVRLVDDK
                     GKVLCAKGQKIENATLDEIPARYWPELQVEEAGKTQEKLEQLATQLQEDVNAIEALYR
                     DKISKLTKGDELPPGVIKMVKVYVAIKRKLSVGDKMAGRHGNKGVVSRVLPEEDMPFL
                     ADGSPVGIVLNPLGVPSRMNVGQILETHLGGAALYLGKQIDEMIQREWTGDALRAKMK
                     KVFTDPHHIELLDSLDDKGLLRYAQTLREGVHVATPVFDGAKEVDIKALLTQANLNTN
                     GQATLFDGKTGEAFDHDVTVGVMYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQF
                     GGQRLGEMEVWAMEAYGAAYGLQEFLTVKSDDVIGRTRMYEAIVKGEHVLESGLPESF
                     NVLLKELQSLCLNVELIEDPNAPRKQESTLELMAREVAAKASAAAAQAAAASASTAPA
                     AQASELGGE"
BASE COUNT          453 a          670 c          711 g          301 t
ORIGIN      
        1 gccaagcgcg acggcatcgt cgaatccgtc gacgccaccc gcatcgtcgt ccgtccgact
       61 caggagagcg acgacgatcc gctgtcggcc aagcccgaca tctacaacct gaccaagttc
      121 cagcggtcga accagaacac ctgcatcaac cagaagccga tcatcgagcg gggcgacccg
      181 gtcaagatgg gcgacgtcat cgctgacggt cccgcgaccg agcggggcga gctggcgctg
      241 ggcgagaaca tcctggtcgc gttcatgccc tggggtggct acaacttcga ggactcgatc
      301 ctcgtcagcg agaacctggt caagaacgac cacttcacct cgattcacat cgaggagttc
      361 gagtgcgtcg cgcgcgacac caagctgggc aaggaagaga tcacctgcga catcccgaac
      421 gtcggcgagg aggccctcaa ggacctcgac gagtccggca tcgtgcgcat cggcgccgag
      481 gtgaagccgc aggacatcct ggtcggcaag atcacgccga agggcgagac ccagctgtcg
      541 ccagaagaga agctgctccg cgcgatcttc ggcgagaagg ccggcgacgt gcgcgacagc
      601 tcgctgcgcg tcccgccggg cgtgcagggg atcgtcatca gcgccaaggt gttctcgcgc
      661 aagggaaccg agaaggacga gcgcgcgaag gacatcgagg atcaggagaa ggagaagctt
      721 ctcgctgacc agcgcgacga ggtgaagatc gtctccgagt cgtatcacgc caagatgcgc
      781 aagctgctgg tcggcaagac cacggccgtg cgtctggtcg acgacaaggg gaaggtcctc
      841 tgcgccaagg ggcagaagat cgagaacgcc acgctggacg agattccggc gcgctactgg
      901 cccgagctgc aggtcgaaga ggccggcaag acccaggaga agctcgagca gctggctacc
      961 cagctgcaag aggacgtcaa cgcgatcgag gcgctttacc gcgacaagat ctcgaagctc
     1021 accaagggcg acgagctccc gccgggcgtc atcaagatgg tcaaggtcta cgtggccatc
     1081 aagcgcaagc tgtcggtcgg cgacaagatg gccggtcgtc acgggaacaa gggcgtcgtt
     1141 tcgcgcgttc tgccggaaga ggacatgccg ttcctcgccg acggctcgcc ggtcggcatc
     1201 gtcctcaacc cgctgggcgt tccgtcgcgt atgaacgtcg gtcagatcct cgagacgcac
     1261 ctgggcggcg ccgcgctgta cctgggcaag cagatcgacg agatgatcca gcgcgagtgg
     1321 accggggatg cgctgcgcgc gaagatgaag aaggtcttca ccgacccgca ccacatcgag
     1381 ctgctggaca gcctggatga caaggggctg ctccggtacg cgcagacgct gcgcgagggt
     1441 gtccacgtcg cgacgccggt gttcgacggc gccaaagagg tcgacatcaa ggcgttgctg
     1501 acgcaagcca acctcaacac caacggacag gcaaccctgt tcgacggcaa gacgggcgaa
     1561 gccttcgacc acgacgtcac ggtcggcgtc atgtacatgc tgaagctcca ccacctggtc
     1621 gacgacaaga tccacgcgcg ttcgatcggg ccgtactcgc tggtcaccca gcagccgctg
     1681 ggcggtaagg cgcagttcgg cgggcagcgg ctcggcgaga tggaggtctg ggcgatggag
     1741 gcgtacggcg cggcttacgg cctgcaggag ttcctgacgg tcaagtccga cgacgtcatc
     1801 ggccggacgc gcatgtacga ggccatcgtc aagggcgagc acgtgctgga gtcgggtctg
     1861 cccgagtcct tcaacgtgct cctcaaggag ctgcagtcgc tctgcctgaa cgtagagctc
     1921 atcgaggatc ccaacgctcc tcgcaagcag gaatcgacgc tggagctgat ggcgcgcgaa
     1981 gtcgccgcca aggccagcgc cgccgcggcc caggccgccg cagcctctgc cagcaccgcg
     2041 ccggcagctc aggcgagcga actgggtggc gagtaacggt ttgacgattc acaatccacg
     2101 gtccgcgaaa aacacagggg caagatgaaa gacat
//
LOCUS       DASWVF010000566         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_40844, whole genome shotgun sequence.
ACCESSION   DASWVF010000566 DASWVF010000000
VERSION     DASWVF010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40844"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGD55_10030"
     CDS             <1..259
                     /locus_tag="VGD55_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0870724.1"
                     /translation="ALRLAELREDPDLLAEIEAAVEDANRVVSRAESIRRFQILDRDF
                     TEAGGQLTPTLKLRRDVVMAEFASEIEGLYSPTGPSAEMAR"
     gene            368..1087
                     /locus_tag="VGD55_10035"
     CDS             368..1087
                     /locus_tag="VGD55_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870725.1"
                     /translation="MVARTPAHVETIGLRVAFGPVVAVHSLDLTVQPASAVALVGRNG
                     AGKSTTLRVLSGVLPATSGQVMVAGVDVTRDPARAKAHVGYCPDVGGLIPRATPWEHL
                     ALAAKLRGLGPDWQDRARDLLELFDLVGAADRVTAGFSHGMGRRLSVVLAVLHDPSVL
                     LLDEPFDGVDPLGVDATLEVISQARANGSAVVVSTHLLELAAAACDEVVVLRSGQIVA
                     AGPSAELRGEAGELRYRALLA"
     gene            1084..>1813
                     /locus_tag="VGD55_10040"
     CDS             1084..>1813
                     /locus_tag="VGD55_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870726.1"
                     /translation="MTGAQLPALVRLRWQMVRAERVRVGLLVLAACIPGLMCGAVIAG
                     QLAPRSHSFDVTVLGPTAFLGFAVLSLVAPLAAGGGNELFPPEQLVAYPIRPATHFFE
                     SLLVAPLNLAWMSQFILLFGMTSFVTGARPGLGLALVTTLVYVAMVTVAGQMVAWILI
                     GARQSVLGRRVVWALTAAIVVTTLVVLQQHLGYRVLDNSPTRPAVSAVLAGGEHRYAA
                     WAAGTVALGVILFGCLWCGPRVCAW"
BASE COUNT          237 a          615 c          626 g          335 t
ORIGIN      
        1 ggcactgcgc ctagccgaac tgcgcgaaga tcccgacctg ctcgcggaga tcgaggcggc
       61 cgtcgaggac gccaatcgcg tggtctcgcg tgctgagtcg attcggcgct tccagatcct
      121 ggatcgcgac ttcaccgaag caggcggtca gctcacgccg acgctcaaat tgcgacgcga
      181 cgtcgtcatg gcggagttcg ccagcgagat cgaaggtttg tattcgccca ccggaccaag
      241 cgccgagatg gcacgctgac tttgggagga catttcgggg gaagccaggc ggtgccttca
      301 gggggatgca gtctcggtcg agcagacatg cgagatcgcg tggtgtttct ggtgccggag
      361 gtgagcagtg gttgcccgca cgcccgccca cgtcgagaca atcggcttgc gggtcgcatt
      421 tggccccgtc gtcgccgttc actcactcga cctgaccgtg cagcccgcgt cagcggtcgc
      481 cctcgtgggc cgcaatggcg cgggtaagtc gacgaccctg cgagtgctct ccggcgtgct
      541 cccggccacc agcggccagg taatggtcgc cggcgtcgat gtcacccgcg acccggcgag
      601 agccaaggcg cacgtcggat actgcccgga cgtcggcggg ctgatccccc gcgcaacgcc
      661 gtgggagcac ctcgctttgg cggccaagct gcgagggctc ggacctgact ggcaagaccg
      721 cgcgcgcgac ctgctcgaac tgttcgacct cgtcggcgcc gccgatcggg tcaccgcagg
      781 cttctcccac ggcatgggac gtcgactctc ggtcgtgctc gccgtcttgc acgatccttc
      841 cgtgttgctg cttgacgagc cgttcgacgg tgtcgacccg ctcggtgtcg acgccacgct
      901 cgaagtgatt tcacaagctc gcgccaatgg ttccgctgtg gtcgtctcga cgcacttgct
      961 cgaactcgcg gccgcagcgt gcgatgaggt ggtggtgctg cgctcagggc agatcgtcgc
     1021 cgccggtcca tccgccgagc tgcgcggcga ggccggcgag ctgcgctacc gagccctgct
     1081 cgcatgaccg gggcgcaact tccggcgctc gttcggttgc ggtggcagat ggtgagggcc
     1141 gagcgcgtcc gggtcgggct actcgtgctg gccgcctgta tccccgggct gatgtgcggc
     1201 gcggtcatcg ccggccagct ggcgccacgc tcccactcct tcgacgtgac ggtgctcgga
     1261 cccaccgcat tcctcgggtt cgcggtgctc tcgctggtcg cgccactcgc agccggcggg
     1321 ggtaacgaac tgttcccgcc tgaacaactg gtcgcgtacc cgatccggcc agccacccac
     1381 ttcttcgagt cgttgctcgt cgcgccgttg aacctcgcgt ggatgagcca gttcatcctg
     1441 ctgttcggca tgacgtcgtt cgtgaccggc gcccgccccg gcctcgggct cgcgctggtt
     1501 accactcttg tgtacgtcgc catggtcacg gtcgccgggc agatggtcgc gtggatcctg
     1561 atcggtgccc ggcagagcgt gctcggcagg cgcgtggtgt gggccctgac agccgcgatc
     1621 gtggtgacga cgctggtcgt gttgcagcag cacctcggct accgggtcct cgacaactcg
     1681 ccaacccggc cggccgtgtc ggccgtgctc gccggcggcg agcaccgcta cgccgcgtgg
     1741 gcggccggca cggttgcgct cggagtcatt ctgttcggct gtctttggtg cgggccacgg
     1801 gtctgtgcgt ggg
//
LOCUS       DASWVF010000567         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_270508, whole genome shotgun sequence.
ACCESSION   DASWVF010000567 DASWVF010000000
VERSION     DASWVF010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270508"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      14..145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            136..1485
                     /locus_tag="VGD55_10045"
     CDS             136..1485
                     /locus_tag="VGD55_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY0870727.1"
                     /translation="MAATAQAQVEAPLTLDEPAAKALGFFDQVGLWGNLGVSLLGFTG
                     ALFVLQPRADSPGLGLAAALLATFVGTALGAAAVGLAAIPGARTGAPSMVLLRGLFGA
                     RLSYLPTGLNILQLVGWGTFEIVIISSAAHQLWPTMPQNGYVVIAGVITTALALRPLG
                     VVRVLRKYVTAAVAIVVVYLFVQLLRHPVPPVPHSSWRNFWPGVDIALAVAISWVPVA
                     ADYSRHSKSGRTAFGAAFVGYTITQTACYALGIIAILSVPAASDGSNHGVWGAFIAVF
                     LGKAAFAVLAIREIDQSFCDVYSTAVSTQNLRPHWDRRVIAFIVGTAATLLALVFHIA
                     NYQNFLFLIGSVFVPLFGVLVVDYFVLKGHRSWDVAERAPSRWLMLLPWAAGFCVYQL
                     VNPGAIPRWAGFWTRIARDIDFTPQTWMSASLLSFIAAALLTLMVRSGGAHCDRAGR"
     gene            complement(1531..1851)
                     /locus_tag="VGD55_10050"
     CDS             complement(1531..1851)
                     /locus_tag="VGD55_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY0870728.1"
                     /translation="MLIVTTNDLPGYRVDEVLGEVFGLTVRSRNAFSQMGAGLKSILG
                     GELRGMTTALTDSRNQVMQRLIDEATARGANAILAMRFDTSEMGGVWTEICAYGTAVR
                     VTKL"
     gene            1905..>2787
                     /locus_tag="VGD55_10055"
     CDS             1905..>2787
                     /locus_tag="VGD55_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0870729.1"
                     /translation="MTLVSDNLLAARALDSMADAVVVGDNEGRVRLWNLGAERLFGWT
                     SKDVVGGPFPGIPDQQAERERDVALARVMSGEHVSLATKRRRADGSVVDVWIVFSPMR
                     AWLDGPIDGWLAVLRDATQQRAVQRELRRRAELVGRLATVVATLNSDLDLGTVLRRIS
                     ESGRELLAADGAAYVVLDGDDLVIAAVSELNSALVGERIPLAESAVATLLQSGRSSMA
                     LNNSDYPNTSLLVASTIRRLPRFAIGLTRLDGLPSGALYVFFAAERRAVGRAELGVLE
                     LLADTAGAALTNARAFDR"
BASE COUNT          385 a          940 c          939 g          523 t
ORIGIN      
        1 gcgtacgctg gttggcgcac accgcgggag ctcgggcgaa accgggctga gagggcggct
       61 ggccgggatc tccggcctaa acgggccgcc gaccgctcga acctgaccgg gtaatgccgg
      121 cgtagggagt ggcagatggc agcaactgcg caggcgcagg tcgaggcgcc gctcacgctc
      181 gacgaaccgg cggcgaaggc gctcggcttc ttcgatcaag ttggcctttg gggcaacctc
      241 ggtgtcagcc tgctcggctt caccggcgcc ctcttcgtgc tccagccccg tgccgacagc
      301 cccggcctcg gcctcgccgc ggccttgctc gcgaccttcg tcggcacggc cctcggtgca
      361 gcggccgtcg gcctcgcagc tatcccggga gcacgcactg gcgcgccgtc gatggtattg
      421 ctgcggggcc tcttcggagc gcgcctctcc tacctgccga ccggtctgaa catcttgcag
      481 ctcgtcggtt ggggcacgtt cgagatcgtc atcatctcct cggccgccca ccagctctgg
      541 ccgacgatgc cgcagaacgg gtacgtcgtc atcgcaggtg tcatcacgac agccctcgcg
      601 ctacggccgc tgggcgtcgt ccgggtgctt cggaaatacg tgacagcagc ggtcgcgata
      661 gttgtcgtct acctcttcgt acagctgctg cgacatccgg tccccccggt cccacacagc
      721 tcgtggcgca acttctggcc tggcgtcgac atcgcgctgg ccgtcgcgat ctcgtgggtg
      781 ccggtcgcgg ccgactactc gcgtcattca aaatcaggcc ggacggcgtt cggtgccgcg
      841 ttcgtcggct acacgatcac ccagacggcg tgctacgcac tcggcatcat cgcgatcctg
      901 agcgtgcccg ccgccagcga cgggtcgaac cacggcgtgt ggggagcgtt catcgcggtg
      961 ttcctcggta aagctgcgtt cgcggtgctg gcgatccgtg agatcgacca atcgttttgc
     1021 gacgtgtact cgaccgcggt aagcacccaa aacctgcgcc cgcactggga tcgccgcgtg
     1081 atcgcgttca tcgtcggcac ggccgcgacg ctgttggccc tggtgttcca catcgcgaac
     1141 taccagaact tccttttcct gatcggctcg gtgttcgtgc cgctgtttgg cgtcttggtc
     1201 gtcgactact tcgtgctcaa agggcatcgc agctgggacg tcgcggagcg ggcgccgtcg
     1261 cgttggctca tgttgctgcc ctgggcggcc ggcttctgcg tgtaccaact cgtgaatcct
     1321 ggcgcgatcc cacgctgggc aggcttttgg actcgcatcg cgcgcgacat cgacttcaca
     1381 ccgcagacct ggatgagcgc ctcgctgctg tcgttcatcg cagctgcgct gctcacgtta
     1441 atggtgcgca gcggcggtgc tcactgtgat cgtgcgggcc gctgatcggc gtccgagccg
     1501 cactcttgaa gcgcgtaccg cgccaacgct ctagagcttc gtgacccgaa ccgccgtgcc
     1561 gtaggcgcag atctcggtcc agaccccgcc catctctgag gtgtcgaagc gcatggcgag
     1621 gatcgcgttc gccccgcgcg ccgtggcctc gtcgatcagc ctttgcatga cctggttgcg
     1681 gctgtcggtg agcgcggtgg tcatgccgcg caactccccg ccgaggatgg atttgagccc
     1741 ggcacccatc tggctgaacg cgttgcgaga tctgacagtg agcccgaaga cctcgccaag
     1801 cacctcgtcg acccggtagc cgggcaggtc gttcgtcgtg acgatcagca tgtcgagcag
     1861 cctagggcac cggtaacgcg gtcgagcgcc tccgcggtga cacgatgacg cttgtgtccg
     1921 acaacctgct cgcggcccgg gcgcttgact cgatggccga cgccgtcgtg gttggcgaca
     1981 acgagggccg ggtgcgcctg tggaatctcg gcgccgagcg gttgttcggg tggacgtcga
     2041 aggacgtggt cggcggacct ttccccggca tccccgacca gcaggccgag cgtgaacgcg
     2101 acgtcgcgct cgcacgggtc atgtcggggg agcatgtctc gctcgccacg aagcggcgcc
     2161 gggctgacgg cagtgtcgtc gacgtctgga tcgtcttcag tccgatgcgt gcgtggctcg
     2221 acgggccaat cgacggttgg ctcgctgttt tgcgtgatgc aacgcagcag cgggcggtgc
     2281 agcgcgagct tcgccgccgg gctgagctgg tcggccggct ggctacggtc gttgcgacgc
     2341 tgaactccga cctcgacctc ggcacggtcc tgcggcgcat cagcgagtca gggcgcgagt
     2401 tgctcgcagc cgacggcgcc gcctacgtgg tgctcgacgg cgacgatctt gtgatcgcgg
     2461 ccgtctcgga gttgaactcc gctctggtcg gcgagcgcat cccgttggcc gagtctgccg
     2521 tcgcgacgct gctgcagtcg ggccgcagtt cgatggcgct caacaacagc gactacccga
     2581 acacctcgct gctggtggcg agcacgatca ggcgccttcc ccggttcgcg atcggcctca
     2641 cccggctcga cgggctgccc tcgggtgcgc tctacgtctt cttcgcagcc gagcgtcgcg
     2701 cagtggggcg cgctgagctt ggcgttctcg agcttttggc ggacaccgcg ggggcagcac
     2761 ttacgaatgc gcgcgcgttc gaccgga
//
LOCUS       DASWVF010000568         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_295749, whole genome shotgun sequence.
ACCESSION   DASWVF010000568 DASWVF010000000
VERSION     DASWVF010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295749"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /gene="infB"
                     /locus_tag="VGD55_10060"
     CDS             <1..2234
                     /gene="infB"
                     /locus_tag="VGD55_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0870730.1"
                     /translation="GVNRPSPGMMPPRPAPGGSSGPGSYGTRGPSAGGPGAGRSTGGG
                     VGAPRSGFPSARPGGPGAGRGGPSAGPGGAGGPGAGPGGPPGAGGRPGPGGRGRGGGT
                     AGAFGRGPGGRPTRGRKSKKQRRQEFDNMQAPSIGGVQVPRGNGQVLRLPRGSSLSDF
                     ADRINANPASLVQVMFHLGEMVTATQSVNEETLQLLGAELGYDVQVVSPEDEDRELLE
                     SFDLEFGEDEGEEGDLSARPPVVTVMGHVDHGKTKLLDAIRNTNVVAREHGGITQHIG
                     AYQVVAESANGPRAITFIDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTIEAL
                     NHAQAAGVPIVVAVNKIDKEDADPTKVRGQLTEYGLIAEEYGGDTMFVDVSAKSNLHI
                     DALLEAILLTADAALDMRANADQDAQGVAIEAHLDRGRGPVATVLVQRGTLRVGDSIV
                     AGEAFGRVRAMLDEFGETVAEATPSRPVQVLGFTSVPDAGDNFLVVPEDRVARQIAER
                     RSARERNAQLADSRGRRSLEDILERMEKGEVEELRLILKGDVSGSVEALEDALVNLDV
                     GADVELRVIDRGVGAITQNDVNLAVASDAVIIGFNVRPAERVSELIDREGVDVRYYSV
                     IYQAIDEVEAALKGLLKPEFEEVRLGTAEIRDVFRSSKFGNIAGCLVRSGVITRNSKA
                     RLIRDGVVVNDHLQIESLKRFKDDATEVREGYECGIGLGSFNDIKVDDVIETFEMKEK
                     PRA"
BASE COUNT          378 a          773 c          803 g          409 t
ORIGIN      
        1 tcggtgtcaa ccggccctcg cctggcatga tgccgccgcg cccagcgccc ggtggttcca
       61 gcggccccgg tagttacggc acgcgtggtc cttctgctgg cggtccgggt gccggtcgct
      121 caaccggcgg cggtgtcggc gccccgcgca gtgggttccc ctcggctcgt cccggcggtc
      181 ccggtgccgg tcgcggcggc cctagcgctg gccccggcgg tgccggtggt cctggcgcgg
      241 gtcctggtgg accgcccggt gcgggtgggc gtcccggtcc cggtgggcgc ggtcgcggtg
      301 gcggtaccgc gggcgcgttc ggtcgtggcc caggcggtcg gccgacccgt ggtcgcaagt
      361 cgaagaagca gcgacgtcaa gagttcgaca acatgcaggc gccctcgatc ggtggcgtgc
      421 aggtcccgcg cggcaacggg caggtcctgc ggctgccccg aggctcgtcg ttgtcagact
      481 tcgccgatcg catcaacgcg aacccggcat cgttggtgca ggtcatgttc catctcggtg
      541 agatggtgac cgcgacccag tcggtgaacg aagagacgtt gcagctcctt ggtgccgagc
      601 tcggctacga cgttcaggtc gtcagccccg aggacgaaga ccgcgagttg ctcgagagct
      661 tcgatctgga gttcggcgag gacgaaggcg aagagggcga cctctccgct cgtccgcccg
      721 tggtcaccgt catgggtcac gtcgaccatg gcaagaccaa gctgctagac gcgatccgca
      781 acaccaacgt ggtcgcgcgc gagcacggcg gcatcaccca gcacatcggt gcctaccagg
      841 tcgttgccga gtcggcgaac ggtccccggg cgatcacctt catcgacacc ccgggccacg
      901 aggcgttcac cgcgatgcgt gcacgtggtg cgcaggtgac cgacatcgcg gtgctggtgg
      961 tagctgctga cgacggtgtg atgcctcaga cgatcgaggc actcaaccat gcccaagcag
     1021 ccggcgtccc gatcgtcgtc gccgtcaaca agatcgacaa ggaggacgcc gacccgacga
     1081 aggtccgcgg ccagctgacc gagtacggct tgatcgctga ggagtacggc ggcgacacga
     1141 tgttcgtcga cgtctcggcg aagtcgaacc tgcacatcga cgcgctcctt gaagcgatcc
     1201 tgctcaccgc agacgccgcg ctcgacatgc gggcgaacgc cgatcaggac gctcaaggtg
     1261 tggccatcga ggcgcacctc gaccgtggtc gtggcccggt ggcgaccgtg ctcgtgcagc
     1321 gcggaacgct gcgcgtcggt gactcgatcg tggccggtga ggcgttcggt cgggtgcggg
     1381 cgatgctcga cgagttcggc gagacggtcg cggaagcaac gccgtcgcgc ccggttcagg
     1441 tgctcgggtt caccagcgtt cccgacgctg gcgacaactt cctcgtcgtc ccagaagacc
     1501 gcgttgcccg gcagatcgcc gagcgccgtt cggcgcgcga acgcaatgcg caactcgccg
     1561 acagtcgcgg tcgtcgcagc ctcgaagaca tcctcgaacg gatggaaaag ggcgaggtcg
     1621 aggagttgcg actcatcctc aagggtgacg tctccggctc ggtcgaagct ctcgaggacg
     1681 ccttggtgaa tctcgatgtt ggtgcggacg tcgaactgcg ggtcatcgac cgcggggtcg
     1741 gcgccatcac ccagaacgac gtcaacctcg ctgtcgcgtc cgacgccgtc atcatcggct
     1801 tcaacgtgcg gccggccgag cgggtcagcg aactcatcga tcgcgaaggc gtcgacgtcc
     1861 gctactactc ggtcatctac caggcgatcg atgaggtcga ggcggctctg aagggcctgc
     1921 tcaagcccga gttcgaagag gtgcgtcttg gtactgccga gattcgcgac gtcttccgct
     1981 cgagcaagtt cggcaacatc gccggctgtc tcgttcgctc gggcgtgatc acccgcaact
     2041 ccaaggcccg cttgattcgc gatggtgtgg tcgtcaacga ccatctccag atcgaatcgc
     2101 tcaagcgttt caaggacgac gccactgagg tccgcgaagg gtacgagtgc ggtatcgggc
     2161 tcggctcgtt caacgacatc aaggtcgacg acgtgatcga gacgttcgaa atgaaggaga
     2221 agcctcgggc ttagtaggcc tcgcgtcggc cttcggccta acgccctccc ttggactgct
     2281 tcgctttacg tccttcggaa gggcattagg cctcggcctc cgtcgatggc ccgctacctc
     2341 cgaggtccct gcccttcctt tcg
//
LOCUS       DASWVF010000569         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_372315, whole genome shotgun sequence.
ACCESSION   DASWVF010000569 DASWVF010000000
VERSION     DASWVF010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372315"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1470)
                     /locus_tag="VGD55_10065"
     CDS             complement(70..1470)
                     /locus_tag="VGD55_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0870731.1"
                     /translation="MPKRRTGLATRIALVTTLAAAIAVLIAGLVSARVISRAAETTGH
                     KVLEQYANLSVAAADLSRTGRVAVPAKIIALLHTQQVQLLVVQPDGRVTGPEGAVTAN
                     GIVTPAEVAALKAGNNFSDLRRLSGGRYYLEGRPITTGTAAGGGVVLVQKVADARSAG
                     GSVGLRLLIALAAGLVVAAFTGVLLARRLARPLQHAAGAARRLSTGARDVRLVPEGPA
                     EVAAVADALNALSTALATSEGRQREFLLSISHELRTPLTAVKGYAEALADGVVPAAAV
                     EATGHVLLAESSRLERLVSDLLDLARLGAEDFRIDITRLDLVVLVRQASLVWADRCAR
                     EGVEFRLEVPPAPVIVDTDPTRVRQIIDGLAENALRVTPSGRPIVFAVGATSYEAVMQ
                     VRDGGPGLTEDDCRVAFDRSALYDRYRGVRRVGTGVGLALVAGLTARLGGRASAGRSP
                     EGGACFTITLPGRPGV"
     gene            complement(1463..2173)
                     /locus_tag="VGD55_10070"
     CDS             complement(1463..2173)
                     /locus_tag="VGD55_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870732.1"
                     /translation="MSQTSGPDGDGTSKGLVLVIEDERAIADLERMYLTREGSGVHVE
                     SDGRAGLAAARNLHPVAIVLDVGIPIIDGTQVCRMLRADGNWTPILFVTARDEEVDRV
                     LGLELGADDYLTKPFSPRELVARVKAVLRRSSSEAASQERVLVVGAVTVDPARRRVMA
                     NGEPIELTATEFELLEFLCRRPGRVFPREHLLSEVWGYEAAAGTRTVDVHVAQLRAKL
                     GDASPIRTVRGVGYSADA"
BASE COUNT          411 a          855 c          826 g          369 t
ORIGIN      
        1 acctgtcggg tgctgctgga acctttctca gggtagcggc cgggctcgta gccccctccg
       61 accccccggc tagacgcccg ggcggcccgg caacgtgatc gtgaaacacg cgccgccttc
      121 cggcgatctg ccggcgctgg cgcggccacc gaggcgagcc gtcaggcccg ccacgagggc
      181 aagaccgacg cccgttccga cccggcgcac accgcggtaa cggtcgtaca gagcagaccg
      241 atcgaaggcg acgcggcagt cgtcttcggt caggccgggg cctccgtcgc gaacctgcat
      301 cacggcctca tacgaggtcg caccgacggc gaacacgatg ggccgaccag acggcgtcac
      361 tcgcagtgcg ttctcggcga gaccgtcgat gatctgccgc accctggtcg ggtcggtgtc
      421 gacgatgaca ggagcaggtg gcacctcgag tcggaactcg accccttctc gagcgcatcg
      481 atcggcccag accagcgatg cctgcctgac cagcacgacg agatcgagcc gggtgatgtc
      541 gatcctgaag tcctcggcac cgaggcgcgc aaggtcgagc aggtcggaga caagtcgttc
      601 gagacggctt gattccgcca gcaacacatg accggtcgct tcgactgctg ccgccggcac
      661 cacgccgtcc gccaaagctt ctgcgtagcc cttcaccgcc gtcagcggcg tgcggagctc
      721 atgcgaaatc gacagcagaa actcgcgctg cctgccctcg ctcgtcgcga gagcagtgct
      781 caaagcgttc agtgcgtcgg cgacggcggc aacttcggca ggaccttcag gcacgaggcg
      841 gacgtcgcgg gcacccgtcg acagtcgcct cgcggccccc gcggcatgct gcaagggccg
      901 ggcaagtcga cgcgcgagca gcactccggt gaacgcggca accacgagac cggctgccaa
      961 ggcaatgagc aaacgcaggc cgaccgaccc gccggcagag cgcgcgtcgg cgaccttctg
     1021 caccaacacg acgccgccgc ccgcagccgt accggtcgtg atcgggcgac cctcgaggta
     1081 gtaacggccg ccgctgagcc ggcgcaggtc cgagaagttg ttgcctgcct tcagcgccgc
     1141 gacctccgcg ggggtgacga tcccgttggc agtcaccgcc ccctcgggac cagtcaccct
     1201 gccgtcgggt tgcacgacga gcagttgcac ctgttgcgta tgcaacagcg cgatgatctt
     1261 tgcgggcacg gctacccgac cggtgcgcga gaggtctgct gcggccaccg acaagttggc
     1321 gtactgctcg agaaccttgt gccccgtggt ctcggccgcc ctgctgatga ctcgcgccga
     1381 taccaaaccc gcgatgagaa cggcgatggc cgcggccaac gtcgtcacca gtgcgatgcg
     1441 ggttgccagc ccggtgcggc gtttaggcat cggcgctgta tccgacccca cgcacggttc
     1501 ggatcggact cgcatcaccg agcttggcgc gaagctgtgc gacatggacg tcgacagtgc
     1561 gggtgccggc ggcggcttcg tatccccaca cctccgacag caggtgctcg cgcgggaaga
     1621 cccgaccggg acggcggcac aggaactcga gcagctcgaa ctcggtcgcg gtcagctcga
     1681 tcggttcgcc gttggccatg acccgtcggc gcgcagggtc gacggtgacg gcaccgacga
     1741 cgaggacacg ttcctgcgac gctgcctcac tgctgctgcg ccgcaacacc gccttcacgc
     1801 gggcgaccag ttcgcgcggg ctgaacggct tggtcaggta gtcgtccgcg ccgagctcaa
     1861 gaccaagtac tcgatcgact tcttcgtcgc gggcggtgac gaacaggatc ggcgtccagt
     1921 tgccgtcggc tcgcagcatg cgacagacct gggtgccgtc gatgatcgga ataccgacgt
     1981 cgagcacgat cgcaaccgga tgcaggttgc gggcagccgc gagccctgcc ctgccgtcgg
     2041 actcgacgtg cacgccggaa ccttcgcggg tgaggtacat ccgttcgagg tcggcgatgg
     2101 cccgctcgtc ctcgatgacc aggacgaggc ctttggatgt gccgtcgccg tccggccctg
     2161 acgtctggct catcgtggtc ctcccgatac ggacgctaga gcagcgtcga cgtctagagc
     2221 ctcgacttga gcacgcaacg ccgtgttaga ggaatgttcg aggaatgttc gggcccgcgg
     2281 gcaggtgagt taggcggtcg ggtcgtcgcc ctcgtcgagg cggtcccaca tgccgacctc
     2341 ggtcaggggg gcgttcttcg ccggccccga ttcgtagcgt cggcccatcg agggccaccg
     2401 gccgctgcgc gtgacggcga gcaggccaca gcccgtcccg agcaacccag ccgccagggg
     2461 c
//
LOCUS       DASWVF010000570         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_15719, whole genome shotgun sequence.
ACCESSION   DASWVF010000570 DASWVF010000000
VERSION     DASWVF010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15719"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /locus_tag="VGD55_10075"
     CDS             <1..1644
                     /locus_tag="VGD55_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY0870733.1"
                     /translation="VGDQPYDTPLLADGVTAGSPAANTGDADAESATPHSTSGRLGVS
                     GAGLPRRAEQLLLSGDITYHLPPPTMLREGSHARARTKANDTVVEALTTVLQQFEIDA
                     TVTGFTRGPTVTRYEVELGPAVKVERITALSKNIAYSVASADVRILSPIPGKSAVGIE
                     IPNADRDVVSLGDVLRSQVATSDHHPMVVALGKDVEGRFICANLAKMPHILVAGATGS
                     GKSTCINTIVTSILTRATPDEVRMVLVDPKRVELTSYEGIPHLVTPIITNPKKAAEAL
                     QWVVREMEMRYDDLAASGYRHIDDFNRAVHSGTLVPPPGRERVYRPYPYLLVVVDELA
                     DLMMVAPRDVEDSIVRITQLARAAGIHLVLATQRPSVDVVTGLIKANVPSRLSFATSS
                     LADSRVILDQAGAEKLVGLGDALFLPMGASKAIRLQGAFVSESEIEAVVSHCKEQLQP
                     TYRDDVTMPAAAKREADDDVGDDLDLLCQAIELVVTTQFGSTSMLQRKLRVGFAKAGR
                     LMDLMESRGIVGPSEGSKARDVLAKPDELDDIVATLRGE"
BASE COUNT          327 a          559 c          591 g          323 t
ORIGIN      
        1 gtgggtgacc agccgtacga caccccgctg cttgccgacg gggtgaccgc cggatcgcca
       61 gctgcaaaca caggcgatgc ggacgccgaa agcgcgacgc cgcactcgac gtcagggcga
      121 ctcggcgtgt cgggggcggg ccttccgcga cgagccgagc aactgctcct gtcgggcgac
      181 atcacctatc acttgccgcc accgacgatg ctgcgtgagg gctcccatgc gcgcgcgcgc
      241 acgaaggcga acgacacggt cgtcgaggca ctcacgactg tccttcaaca gttcgagatc
      301 gatgccacgg tgacggggtt cacgcgaggc ccaacggtga cgcgctacga ggtcgagctc
      361 ggccctgcag tgaaggtcga gcgcatcacc gcgttgtcga agaacatcgc gtactcggtc
      421 gctagcgctg acgtgcggat cttgtcgccg atcccaggca agtcggccgt cggcatcgag
      481 atcccgaacg ccgaccgcga cgtggtcagc ttgggtgacg tgttgcgatc gcaggtcgcg
      541 acctcggatc accacccgat ggtggttgcg ctcggcaagg acgtcgaggg tcggttcatc
      601 tgcgcgaact tggcgaagat gccacacatc ttggtggctg gtgcgaccgg gtcagggaag
      661 tcgacgtgca tcaacacgat cgtgacgtcg atcctcacac gagctacgcc cgatgaagtg
      721 cgcatggtgc tggtcgaccc caagcgggtc gaattgacaa gttacgaagg aattcctcac
      781 ctcgtcacac cgatcatcac gaaccccaag aaggccgcgg aggccttgca atgggttgtc
      841 cgcgagatgg agatgcgcta cgacgacctc gcggcgtcgg gctaccggca catcgacgac
      901 ttcaatcgcg cggtccactc cggaacgctc gtgccgccgc caggccgtga gcgtgtatat
      961 cggccttacc cctacctgct ggttgttgtc gacgagctcg ccgacctgat gatggtcgct
     1021 ccccgcgacg tcgaagactc gatcgtgcgc ataacgcagc tcgcgcgggc cgctggtatt
     1081 catctcgtgt tggccaccca gcggccgagc gtcgatgtcg tgaccggact catcaaggcg
     1141 aacgtgccgt cgcgcctttc gttcgccacg tcgagcctcg ccgacagtcg agtgatcctt
     1201 gaccaggccg gtgccgagaa gttggtcggt ctcggcgatg cgctgttcct cccgatgggt
     1261 gcgagcaagg cgatccgctt gcagggtgcg ttcgtatcgg agagcgagat cgaagcggtc
     1321 gtcagtcatt gcaaggagca gttgcagccg acgtatcgag atgacgtgac gatgcccgcc
     1381 gcggcgaagc gggaagccga cgacgatgtc ggcgacgacc tcgacctgct gtgccaagcc
     1441 atcgaactcg tggtgaccac gcagttcggc tcgacgtcga tgctccagcg caagctgcgg
     1501 gtgggcttcg cgaaggcggg tcggctgatg gacctcatgg aaagccgagg catcgtgggc
     1561 ccgagtgagg gctcgaaggc cagggacgtc ctcgccaaac cggatgaact ggacgatatc
     1621 gtcgcgaccc ttcgcgggga gtgacacgcg cgatccaggg aagaatctcc ctagggtttg
     1681 cttcgcgtac ccaggccgtc aggtttcccg tcactttgtc gacacctagt actggtcata
     1741 gactcggcgc tgtcgacctt tttgtgcgat gcgtgtgtcc acctgcggtg ttcaaatgcg
//
LOCUS       DASWVF010000571         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_474716, whole genome shotgun sequence.
ACCESSION   DASWVF010000571 DASWVF010000000
VERSION     DASWVF010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474716"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..892
                     /locus_tag="VGD55_10080"
     CDS             404..892
                     /locus_tag="VGD55_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870734.1"
                     /translation="MSALPLIGNLAWAPVPAIESVEVLDRFNGVPTFGLFSAGGERQL
                     FWRVTCYVPRSYSVWLYVPLTTDDESRLAHAEPSDLLGGLIFGSSAPRYATVGVAMDY
                     RLVFEREWQVPQDIDTQRLLDDVLRFLLDALTIALSEDMPAARREIVYTTSEAIRELV
                     AA"
     gene            complement(980..>2262)
                     /locus_tag="VGD55_10085"
     CDS             complement(980..>2262)
                     /locus_tag="VGD55_10085"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEY0870735.1"
                     /translation="VETGQRLRDPGNVFVQDCIDRMAATHVLPRAVLAEQVKGAAGYL
                     DQRLLRTVVEQNFPDPRALDEWVPKQDFTGRKSFVRAFAPRLIHVLPGNSPGVAVKSI
                     AQGALVKAVNLFKMSSSDPFTTVAVLRTMADIDPDHAIVKSMSAVYWRGGDDAVERAL
                     YRPQYFDKIVAWGGGDAISNVVKYLGPGFQLVSFDPKTSISMIGREAVASDEALDRAA
                     DLAAGDVMVLNQEACVASRFIFIEANHDDADRFCGRLHARIVERAAASGDARPLDLDL
                     REQVDMLMMLDDEFRVWGKTDGKGLVIRSDEPVDFHPINKTANVVVVRSLDDAARYVN
                     VATQTVGFFPFDRMPAYRDLLASGGAQRIVRLGEAGPSTIGNPHDAMYPLHRFVHWMA
                     HEDGAEGPADLQRGRAETVVDLRRPLGRCLGMDS"
BASE COUNT          379 a          781 c          701 g          401 t
ORIGIN      
        1 ccggccggcg accgccgcgc ctgaccgtga aagaaggccg accgggcacg aacggtcgtt
       61 tgagccaatg atttgatcgg ctacctcgtg ccaggtttac acttgtatag tcgactacca
      121 tcgacagagt atgcgaatca actacttaac ccgaccatgc agcaaggccg cggcggtcct
      181 ggtcactcgt tacctcgctc caggtgccac aactcgcgtt acgaccgccc gacgcctgcg
      241 cgaggccggc tttcgcgtgc tccacagtcc caccaagaca aaccggctcc atgtgagcgt
      301 cttcccgccg ctgacaaccg ctggcgagcc tgccgactgg ggcagccaac tggcggccct
      361 gttcaacgca tgcttcactt gagtcaagaa gagaggacag gacatgagcg cgctgccgtt
      421 gatcggcaac ctggcctggg ctccggtgcc cgcgatcgag agcgtagagg tgctcgaccg
      481 ctttaacggc gtgcccacat tcggtctgtt cagcgcaggt ggagagcgac agctgttctg
      541 gcgcgtcacc tgctacgtcc cgcgttccta ctccgtctgg ctgtacgtcc cgctcaccac
      601 tgacgacgag agccgcctag cgcacgccga gccatcggac cttctcggtg gcctcatatt
      661 cggttcatcg gctccccgtt acgcgactgt tggcgttgcc atggactacc gtctggtttt
      721 cgagcgcgag tggcaggtcc cgcaggacat cgacacccag cgactgctcg acgacgtcct
      781 caggttcctg ctcgacgcgc ttaccatcgc cctcagcgag gacatgccgg ccgctcgccg
      841 ggaaatcgtg tacacgacct ctgaagctat ccgcgaactt gttgcagcct aagtctgacc
      901 agcgtgaact cggttatgaa ggcatctggg ttggtgtagc tgagcgactg actgccagac
      961 cgtgtcaact ggcattccac tagctgtcca ttccgagaca tcgcccgagc ggtcggcgca
     1021 ggtcgacaac cgtttcggca cgaccacgct gtaagtcggc cggcccttcg gccccgtcct
     1081 cgtgcgccat ccagtgcacg aagcggtgca ggggatacat ggcgtcgtga gggttgccga
     1141 tggtgctcgg cccggcctcg cccagccgca cgatgcgctg ggccccgccg cttgcgagca
     1201 ggtcccggta ggcgggcatc cgatcgaacg gaaagaaccc gaccgtctgc gtagccacgt
     1261 tcacgtacct cgcggcgtcg tcgagcgagc ggaccacgac cacgttggcg gtcttgttga
     1321 tcgggtggaa gtccaccggt tcgtccgaac ggatcaccag tcccttgccg tcggtcttgc
     1381 cccacacccg gaactcgtcg tccagcatca tcagcatgtc gacctgctcg cgcaggtcca
     1441 ggtcaagcgg ccttgcgtcg cccgaggcgg ccgccctctc gacgatgcgc gcgtgcagcc
     1501 gcccgcagaa gcggtcggcg tcgtcgtggt tcgcctcgat gaagatgaac cggctggcga
     1561 cgcacgcctc ctggttgagc accatgacgt cgccggcggc gaggtcggcc gcgcggtcga
     1621 gcgcctcgtc cgaggcgact gcttcccggc cgatcatcga gatcgacgtc ttggggtcga
     1681 acgacaccag ctggaagccg ggaccgaggt acttgaccac gttgctgatc gcgtcgccgc
     1741 cgccccaggc cacgatcttg tcgaaatact gcggacggta gagcgcccgc tccaccgcgt
     1801 catcgccccc gcgccagtac acggccgaca tcgacttcac gatggcgtga tccgggtcga
     1861 tgtccgccat cgtccgcagg acggcgaccg tggtgaacgg atcgcttgac gacatcttga
     1921 acaggttgac cgccttgacc agcgctccct gggcgatgga cttcaccgcg acccccggcg
     1981 agttgccggg cagcacgtgg atgagccggg gcgcgaacgc gcgcacgaag ctcttgcgcc
     2041 cggtgaagtc ctgcttcggc acccactcgt ccagcgccct cggatcgggg aagttctgct
     2101 cgaccaccgt tcgcagcagc cgctggtcga gatacccggc cgcgcccttg acctgctccg
     2161 ccagcaccgc ccggggcagc acgtgggtgg cggccatccg gtcgatacag tcctgcacga
     2221 agacgttgcc tgggtcgcgg agccgctggc cggtctcgac ca
//
LOCUS       DASWVF010000572         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_346925, whole genome shotgun sequence.
ACCESSION   DASWVF010000572 DASWVF010000000
VERSION     DASWVF010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346925"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="gatB"
                     /locus_tag="VGD55_10090"
     CDS             complement(<1..1206)
                     /gene="gatB"
                     /locus_tag="VGD55_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0870736.1"
                     /translation="MSTAYDEALAAYEPVFGLETHVELGTKSKMFCGCSTTFGAAPNT
                     HVCPTCLGLPGSLPVTNAVAIASTIRIGLALHCSIASWCRFARKNYFYPDMPKNYQIS
                     QYDEPLCVEGYLDVEVNGEVVRVGIERVHLEEDTGKSLHVGGSTGRIHGATHSLVDYN
                     RAGIPLVEIVTKPVPGTGALAPEVARAYVTELRETLRTLDVSDVRMEEGSLRCDVNLS
                     LNPIGATEWGTRSETKNVNSLRSVERAVRSEIERQALVLTGGGRVKQETRHFHEDSGL
                     STSGRSKEQAEDYRYFPEPDLVPIAPADAWIEELRATLPELPFDRRRRLRDEWGISVL
                     DMQAALNAGAVGSIEATVARGVPARDAYKWWMGELARQANERGVEVSELPVSPEQVAR
                     IVALVDAGKL"
     gene            complement(1203..2696)
                     /gene="gatA"
                     /locus_tag="VGD55_10095"
     CDS             complement(1203..2696)
                     /gene="gatA"
                     /locus_tag="VGD55_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY0870737.1"
                     /translation="MTDLTRLTAAQIAAAIASGEASALEVTQAHLDRIDAVDKSLHAF
                     LHIDTEGALAAAAGVDAKRASGHHLGALAGVPLALKDVLTMKGVPTTCGSKILEGWRP
                     PYDSTVVRKLRAADVVILGKTNMDEFAMGSSTENSAYGVTRNPWDLERIPGGSSGGSS
                     AAVASYEAPLAIGTDTGGSIRQPAAVTGIVGVKPTYGGVSRYGLVAFSSSLDQAGPFA
                     RNVFDAALLHAAIAGHDPMDSTSIDAPVPDVVAAALHGDVRGLRIGVVRELNGEGYDN
                     GVRQRFSEAVALLGELGASVGEVSCPHFDYAMPAYYLIAPSECSSNLARFDAMRFGLR
                     IGDDGTHSAEEVMALTRAAGFGAEVKRRIMIGTYALSSGYYDAYYGSAQKVRTLISRD
                     FAAAFENFDVLISPTTPTTAFRIGERSDDPLKMYLADLDTIPSNLAGNASMSVPCGLS
                     PDDGLPVGLQVMAPAMADDRLYRVGAALEAALVGHWGSPLLEKAPAL"
     gene            complement(2700..2996)
                     /gene="gatC"
                     /locus_tag="VGD55_10100"
     CDS             complement(2700..2996)
                     /gene="gatC"
                     /locus_tag="VGD55_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0870738.1"
                     /translation="MPSITRADVAHLARLSRLALTDEELDQMATQLDVIINSVARVSE
                     VAAADVPPTSHSVPLTNVFRADLVVPCLSPEEALAGAPAAEDQRFRVPRILDEE"
     gene            3095..3448
                     /locus_tag="VGD55_10105"
     CDS             3095..3448
                     /locus_tag="VGD55_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870739.1"
                     /translation="MAAAFSASGVVHLVHPATFTRIVPTFLPAKTGLVYVTGVAELIC
                     AAGLWRRDRWAGVATAALLVVIWPANLQMAINAQRGDDSLTKVEDWVRLPLQIPLIWC
                     ALQSGRTRVRRTPEG"
BASE COUNT          615 a         1171 c         1144 g          579 t
ORIGIN      
        1 caacttcccg gcgtcgacga gcgcgacgat tcgagcaact tgctcgggac tcacgggcaa
       61 ctcggagacc tcgacgccgc gctcgttcgc ctgccgcgcc agctcaccca tccaccactt
      121 gtaggcatca cgggccggga cgccgcgggc aaccgtggcc tcgatcgatc cgaccgcccc
      181 cgcattgaga gcagcctgca tgtcgagtac ggagatgccc cactcgtcgc gaagccgccg
      241 ccggcgatcg aaggggagtt cgggcagtgt cgcgcgcaac tcctcgatcc acgcatcggc
      301 cggtgcaatc gggacaaggt cgggctcggg gaagtagcgg taatcctcgg cctgttcctt
      361 acttcgcccc gacgtcgaga ggccgctgtc ttcgtgaaag tgccgcgtct cctgcttgac
      421 gcgacccccg ccggtcagca cgagggcctg ccgctcgatc tccgatcgca ccgcacgctc
      481 gaccgaccgc agcgagttga cgttcttcgt ctcactacgc gtgccccact cggtggcgcc
      541 gatcggattg agcgagaggt tcacgtcaca ccgcagcgag ccctcttcca tgcgcacgtc
      601 ggaaacgtcg agcgtacgaa gggtttcccg caactcggtc acgtacgcgc gagcgacttc
      661 gggcgcgagc gcgccggtgc cgggcaccgg cttcgtgacg atctcgacca gcgggatgcc
      721 ggccctgttg tagtcgacga gcgaatgtgt ggcaccgtga atgcgcccgg tggaaccgcc
      781 gacatgtagc gactttccgg tgtcttcttc gagatgcacc cgctcgatgc caacccgcac
      841 gacctcgccg tttacttcga cgtcgagata tccctcgacg cacaaaggct cgtcgtactg
      901 cgagatctgg tagttcttcg gcatgtcggg atagaagtag ttcttgcgag cgaaccggca
      961 ccacgacgcg atcgagcagt gcaacgctag gccgatgcgg atcgtcgacg cgatcgccac
     1021 cgcgttggtg acgggcaacg aacccggcaa gcccaggcag gttgggcaga catgagtgtt
     1081 gggtgcggcc ccaaacgtcg tcgagcaacc gcaaaacatc ttcgacttcg tgccgagctc
     1141 gacgtgggtt tccagaccga acaccggctc gtaggcggcg agcgcctcgt cgtaagcggt
     1201 actcataacg cgggggcttt ctcgaggagc ggcgaacccc agtgaccgac cagggcggcc
     1261 tcaagtgcgg cgccgacccg gtacagacgg tcatcggcca tcgccggcgc catcacctgg
     1321 aggccgaccg gcaacccgtc gtcgggcgaa agcccgcacg gcaccgacat cgacgcgttg
     1381 cccgcaaggt tcgacgggat cgtgtcgagg tcagcgaggt acatcttcaa cggatcgtcg
     1441 cttcgctcac cgatgcggaa cgccgtggtc ggcgtggtcg gcgagatgag cacatcgaag
     1501 ttctcgaagg cggcggcgaa atcgcgactg atgagcgtgc gtaccttctg cgccgacccg
     1561 taatacgcat cgtagtagcc gctcgacaat gcgtaggtgc cgatcatgat gcggcgcttg
     1621 acctcagccc cgaagccggc tgcgcgggtc agcgccatca cctcctcggc gctatgcgtt
     1681 ccgtcatcgc caatgcgcaa cccgaagcgc atcgcgtcga aacgcgcgag gttcgacgag
     1741 cattcgcttg gtgcgatcag gtagtaggcc ggcatggcgt agtcgaagtg cgggcaactc
     1801 acctcgccga ccgacgcccc gagttcgccg agcaacgcga cggcttcaga aaatcgttgt
     1861 cgtaccccgt tgtcgtaacc ctcgccattc agttcccgca cgacaccgat ccgcaatccg
     1921 cgaacgtccc cgtgcagcgc tgccgcaacg acgtcgggca ccggtgcgtc gatcgacgtg
     1981 gaatccatcg ggtcgtgacc cgcgatcgcc gcatgcagca acgcggcatc gaagacgttg
     2041 cgcgcgaacg ggccggcttg gtcgagcgac gacgagaacg cgacgagccc gtagcgcgag
     2101 actcccccgt acgtcggctt cacgccgacg atgccggtga ccgcagccgg ctggcggatc
     2161 gatccgccgg tgtcggtgcc gatcgcaagc ggcgcctcgt acgacgcgac cgccgcactg
     2221 gatccgcccg acgaaccgcc cggaatccgc tcgagatccc acgggttgcg cgtcacgccg
     2281 tacgcggagt tctcggtcga tgaccccatc gcgaactcgt ccatgttggt cttgccgaga
     2341 atgacgacat cggccgcgcg gagcttgcga acgacggtcg agtcgtacgg cgggcgccac
     2401 ccttcgagga tcttcgaccc gcaggtggtc ggcaccccct tcatggtgag gacgtctttc
     2461 aatgcgagcg ggacgccggc cagcgcgccg aggtgatgac ctgaagcacg tttggcgtcg
     2521 acgccggcgg cagccgccaa ggcaccttcg gtgtcgatgt gcaggaaggc gtgcaaagac
     2581 ttgtcgacgg catcgatgcg atcaaggtgg gcctgcgtca cctcgagggc cgaggcctcg
     2641 cccgacgcga tggcggcggc aatctgggca gccgtcaacc tcgtgagatc ggtcaccgcc
     2701 tactcctcgt ccagaatgcg cggcacccgg aagcgctggt cttctgctgc gggagcgccg
     2761 gcaagagcct cctcgggcga taggcacggc accaccaggt cagcgcgaaa cacgttggtc
     2821 agcggcaccg agtgcgacgt cggcggcaca tcggcggccg cgacctcgct gacccgcgcg
     2881 acggagttga tgatgacgtc gagttgggtg gccatctggt cgagctcctc gtcggtcagg
     2941 gctagacgcg acagccgggc gagatgcgcg acgtcggcgc gggtgatcga tggcacggat
     3001 tcatcttagg gtgccgccgt acagtgccag agtggattac gccgcggtga acgaaagccc
     3061 gaaagaccgt tggcgccacc ggagcccttg gatcgtggcc gctgcgttca gcgccagcgg
     3121 cgtggtgcac ctcgttcacc ccgccacctt cacccggatc gtgccgacct tcttgcccgc
     3181 caagactggc ttggtctacg tgaccggcgt cgccgagttg atctgcgcgg ccggattgtg
     3241 gagacgtgat cgctgggccg gcgtcgccac tgccgcattg ctcgtcgtca tctggcccgc
     3301 caacctgcaa atggcgatca acgcccagcg gggagacgac tccctgacca aggtcgagga
     3361 ctgggtgcgc ctgccgctcc agattccgct gatctggtgc gcgctccaat cgggtcgtac
     3421 acgagtcaga cggacgccgg aggggtgaac tcggcgatcg cggcgtccca gtcgatggcg
     3481 atcgcaccgg gattgtcagc aagcacttt
//
LOCUS       DASWVF010000573         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_449404, whole genome shotgun sequence.
ACCESSION   DASWVF010000573 DASWVF010000000
VERSION     DASWVF010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449404"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..>1539)
                     /locus_tag="VGD55_10110"
     CDS             complement(123..>1539)
                     /locus_tag="VGD55_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0870740.1"
                     /translation="IDLMDDDIDHASILDFPIVYACARAGRASLNRPDDGTMPDSPDL
                     EPLFATILKTVPAPSFDPDVPLRAHVTNLDASQYLGRLALLRVHSGTMKRGQQVAWCK
                     SDGTIEKVRLTELLGTSALERVPIETAGPGDIVAIAGIPEITIGETIADPDDPRPLPI
                     ITVDEPSLSMTVGINTSPLAGESGKKLTARLVKNRLDAELVGNVSIRVLNTERPDTWE
                     VQGRGELQLAILVEIMRREGFELTVGKPQVVTKRVNGKVHEPMERLTIDAPSDYQGVL
                     IQLLALRKGRLEQMVDHGTGWIRMEYIVPARGLIGFRTEFLTETRGTELLHHVHERYE
                     PWHGELRTRPTGSLVADRRGPTTGFALSNLQERGTMFVGPQTEVYEGMIVGENARQDD
                     MDVNPTKEKKLTNMRQSSSDVLVPLIPHKTLSLEQALEFCREDECVEVTPANVRIRKV
                     ELSMSEREKYRGRRAKAARED"
BASE COUNT          253 a          502 c          497 g          287 t
ORIGIN      
        1 gtgcaacctg tgcaacccgt gtacgcggag ccgccacagc ccgctcacgt cgagcccacg
       61 cacgcgccga gtcacgcacg gccgatccac gagacgcagc ccatcccgat cgacccgcag
      121 ctctagtctt cgcgggcggc ctttgcccgg cgtccgcgat atttctctcg ctctgacatc
      181 gacagttcga ccttgcggat gcgcacattc gccggcgtga cttcgacgca ctcgtcttcg
      241 cgacagaact ccagagcctg ctcgagagac aaggtcttgt gcgggatcaa cggcaccaag
      301 acgtcgctcg acgactgacg catgttcgtg agcttctttt ccttggtggg gttgacgtcc
      361 atgtcgtctt ggcgagcgtt ctcgccgaca atcatgccct cgtagacctc ggtctgaggg
      421 ccgacgaaca tggtgccgcg ctcttgcagg ttcgatagcg cgaagccggt cgtcgggccg
      481 cgacggtcgg caacaagcga cccggtcggg cgggtacgca gctcgccgtg ccacggctcg
      541 taacgctcgt gcacgtggtg cagcaactcg gtgccgcgcg tctcggtcaa gaactcggta
      601 cggaagccaa tcagaccgcg agcaggcacg atgtattcca tccggatcca gccggtgccg
      661 tggtcgacca tctgctcgag ccggcccttc cgcagcgcaa gcagctggat gaggacgccc
      721 tgatagtcgc tcggcgcatc gatggtcagt cgctccatcg gctcgtgcac cttgccgttg
      781 acgcgcttgg tgacgacttg cggcttgccg accgtgagct cgaatccttc acgccgcatg
      841 atctcgacga ggatcgcgag ttgaagctcg ccgcggccct gaacttccca ggtgtcggga
      901 cgctccgtgt tcagcacccg aatcgacacg ttgccgacga gttcagcgtc gagccggttc
      961 ttgaccagcc gcgccgtgag tttcttgcct gactcgccgg cgagcggtga ggtgttgatg
     1021 cccaccgtca tcgacaggct cggctcgtcg acggtgatga tcggcagcgg gcgcgggtcg
     1081 tcggggtcgg cgatggtctc accgatggtg atctcgggga tgcccgcgat ggccacgatg
     1141 tcgcccggac ctgcggtctc gatcggcacc cgctcaagag cgctggtacc gagaagctcg
     1201 gtaaggcgca ccttctcgat cgtgccgtcg gacttgcacc acgcaacttg ctgcccgcgc
     1261 ttcatcgtgc cggagtgcac gcgcagcagc gcgagccgac cgaggtactg cgaggcatcg
     1321 aggttagtga cgtgcgcacg cagcggcaca tcggggtcga acgacggcgc cggcaccgtc
     1381 ttgagaatcg tcgcgaataa cggctcgagg tcaggactgt cgggcatcgt gccgtcgtct
     1441 ggcctgttca gtgatgcccg accggcgcgc gcgcacgcgt agacgatcgg gaagtcgaga
     1501 atgctcgcgt ggtcgatgtc gtcgtccatc agatcgatg
//
LOCUS       DASWVF010000574         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_372682, whole genome shotgun sequence.
ACCESSION   DASWVF010000574 DASWVF010000000
VERSION     DASWVF010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372682"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VGD55_10115"
     CDS             complement(<1..1084)
                     /locus_tag="VGD55_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0870741.1"
                     /translation="MSYRQSPLRSSGRGGRGPRGRRGLGAGLILLLVVILAGAGVGAF
                     YLYPRSSHTHTTSGTPGSPSSPASPSVIVSPTNPADALAQQFAQAWSKGDLTGVQYLG
                     GLSGTDVQSAYTTIVQQLQAVSVNVTAQPTQPTSDASIFSSTLNVTWHLVGDQTWTYQ
                     STVDVNNVAGSWQLAWKPSVVQPSLGAGDTLQYTRLAPTRATILDGAGQPIIEARPVV
                     DIGLEAGMVLDPVTTASNMSKILGVDAPSLVTRIKGSTSDEFVDVITLREPDYEKVAA
                     QLAPIPGLVLRDLNEDLSPSHDFARSLLGTVGPVTKEIVDASNGRYISGDVGGLGGLE
                     KDFDATLSGSPGYEIQALHQAAAPTVV"
     gene            1126..1461
                     /locus_tag="VGD55_10120"
     CDS             1126..1461
                     /locus_tag="VGD55_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0870742.1"
                     /translation="MLVYEGVGLLLVVALWIFCLVDVIVSKEDECQNLPKLVWLLIVL
                     MLPDVGSVLWLIAGRPKGLRQSWQQRTLRQATGPGMPRTAPRTRMPSAGSNPDDDEEF
                     LRSIRRPDE"
     gene            1458..1994
                     /locus_tag="VGD55_10125"
     CDS             1458..1994
                     /locus_tag="VGD55_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870743.1"
                     /translation="MIPPRSTRQRWFNSIVAVVIVIAAVIEIRLYVVPASDGARSADA
                     VVVLGGQEYQARLRTADAMLNKYPGSVVVVSIPDGITCPPQPPGASAILCFSPNPSTT
                     QGEARTAAKLAAKYGWKTMEVVTTADQVWRARLRFSRCWSGNLTMVRSDSSIYSRMRA
                     VPYETAATVKAETMQRSC"
BASE COUNT          369 a          645 c          650 g          339 t
ORIGIN      
        1 agaccacggt gggcgcggcc gcttgatgga gcgcttggat ctcatagccc gggctgccgg
       61 agagcgtcgc gtcgaaatcc ttctcgagac ccccaagacc gccgacgtca ccggagatgt
      121 accgcccgtt cgacgcgtcg acaatctcct ttgtgaccgg gccgacggtg ccaagcagcg
      181 atcgggcgaa gtcgtgcgaa ggactcaggt cttcgttcag gtcgcgcaaa accaggccgg
      241 gaatcggggc caactgcgca gcgaccttct cgtaatccgg ctcgcgcaac gtgatgacat
      301 cgacgaactc atcagacgtc gaccccttga tgcgcgtcac caacgacggc gcgtcgacgc
      361 caaggatctt cgacatgttc gacgccgtcg tgactggatc gagaaccatc ccggcttcta
      421 atccgatgtc gacgaccggg cgcgcttcga tgatcggctg cccggcgccg tccaaaatcg
      481 tcgcccttgt cggagcaagg cgggtgtact gcaacgtgtc accggcgcca agtgacggct
      541 gcacgaccga cggcttccag gcgagctgcc aactgcccgc gacattgttg acgtcaacgg
      601 tcgactggta cgtccacgtc tggtctccca ccaagtgcca agtgacgtta agcgtcgacg
      661 agaaaatact cgcatcggac gtcggttggg tcggttgtgc ggtgacgttg accgacaccg
      721 cctgcaactg ctgaacgatg gtcgtatagg cagactgcac gtcggttccg ctgaggccgc
      781 cgaggtactg gacgccggtc aggtctccct tggaccaggc ctgcgcgaac tgctgcgcca
      841 gcgcgtcggc ggggttggtc ggcgacacga tgaccgacgg cgacgcaggc gacgacggcg
      901 atccgggcgt ccccgacgtc gtgtgggtgt gcgacgagcg cggatacagg tagaacgccc
      961 ccacgcctgc gccagccaga atcaccacga ggagcaggat cagcccagcg cccagccctc
     1021 gcctgccgcg ggggccgcga ccccctcggc ctgaagatcg caacggcgac tggcggtagc
     1081 tcacccgatg agcctagtaa tctgtccgcg acgaagggtg ggggcatgct cgtctacgaa
     1141 ggcgtcggac tgcttctggt tgtcgcgctg tggatcttct gcctcgtcga cgtgatcgtg
     1201 tcgaaagagg atgaatgcca gaatctgccc aagctcgtat ggctgctcat cgtgctcatg
     1261 ctgccggacg tcggttccgt cttgtggctg atcgctggtc ggccaaaggg actccggcag
     1321 tcgtggcagc aacggacgtt acgccaggcg acgggcccgg gaatgccgag gacggcaccg
     1381 cgaaccagga tgccctcggc tggatcgaat ccggacgacg acgaggagtt tctgcgaagc
     1441 attcgccgcc cggacgagtg atccccccgc gctcaacccg gcagcggtgg ttcaacagca
     1501 ttgttgcggt cgtcatcgtg atagccgcag tgatcgagat ccggctgtac gtcgtcccgg
     1561 catctgatgg tgctcggtcg gccgacgcgg tcgtcgtgct gggtggccag gaataccaag
     1621 cccgcctaag aacagccgac gcaatgctga acaagtaccc gggttcggtg gtcgtcgtct
     1681 cgatccccga cggcatcacc tgcccgccgc agccgcctgg ggcgtccgcg atcctctgtt
     1741 tcagcccaaa cccgagcacg acgcaggggg aggcgcgaac cgcggcgaag ctcgcggcga
     1801 agtacggctg gaagacgatg gaggtcgtga cgacggccga tcaagtctgg cgggcccgcc
     1861 tgcgcttctc ccgttgctgg tccggaaacc tgaccatggt ccgatccgat tcgagcatct
     1921 actcacgcat gcgcgccgtt ccgtacgaga ccgccgccac ggtgaaagcc gaaactatgc
     1981 aacgcagctg ctgacaaacg ccg
//
LOCUS       DASWVF010000575         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_143609, whole genome shotgun sequence.
ACCESSION   DASWVF010000575 DASWVF010000000
VERSION     DASWVF010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143609"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..134)
                     /locus_tag="VGD55_10130"
     tRNA            complement(61..134)
                     /locus_tag="VGD55_10130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Ile,seq:cat)
     gene            211..2226
                     /locus_tag="VGD55_10135"
     CDS             211..2226
                     /locus_tag="VGD55_10135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY0870744.1"
                     /translation="MFESRGWDDGLDEAQLAAATHGDAPLVILAGAGTGKTRTLVARV
                     AQLVDRGVDPTRILLLTFTRRAADDMLARASTICGDRAGRQLWGGTFHAVAHKLVSAH
                     AETLGLPPALSVLDPADARDLMDLLRSDHHLSGAEQRLPRGDTLVDIYSRAVNTSQPA
                     RDVIASSFPWCDPHTESILELFRAYVARKREHGFLDFDDLLLAWRSLLTDPTLGPTIA
                     TRWDYVLVDEYQDVNQIQVDIVTLLRPGGQGLTVVGDDAQAIYSFRGTDSKHIVELSQ
                     TLTDARVVRLERNFRSRQRLLDLANAVRPSTDGEHLVLHSERTGGPRAQLVRCHDAAS
                     EARLVVDAVLEGAEQGERLRDHAVLMRAAHHSDLLEVELTARGVPFVKYGGLKFLEAA
                     HVKDFVAGLRLLDNRIDEIAWFRLLRLHDGIGPARARALLDSLQPGDAEAEQRHADVA
                     AIAPPGSRVALTAMLDAVVCARQQKAPADRAGAVLEFLRPLLTNRYADHAARLTDLDR
                     LVGAAANAPDLAGFVAELTLDPPASTTDLAGPPHLDDDYLVLSTVHSAKGLEWESVHV
                     IHVVDGAFPSDLALSTKTGLLEEQRLFYVAVTRARDRLSIYTPLRMPHHRFARDDLHS
                     YALGSRFLDDAALAALDVQESVVTRPSIVALPANARVAMPALDELWT"
BASE COUNT          514 a         1010 c          943 g          509 t
ORIGIN      
        1 cgtgaccact caaccgaagg tcgaatgaga ggcatcagcc gaagtaatac agggccgggg
       61 tggggcggct ggggctcgaa cccaggaccg acggattatg agtccgctgc tctgaccggc
      121 tgagctaccg ccccgaggtc cgacacgccg aagcctatcg agcgggcgcg ccttgacccg
      181 gcatcggcgg cgtccggtat tgtcgaacag gtgttcgaat cacgcggctg ggacgacggt
      241 ctcgacgagg cccaactcgc cgcggcgacc catggcgacg cacccctggt gatcttggcc
      301 ggtgccggca ccggcaagac gcgcacgctg gtcgcccgcg tcgcccaact cgtcgatcgc
      361 ggcgtcgacc cgacgcgaat cctgctgctg acgttcaccc ggcgtgccgc tgacgacatg
      421 ctcgcccgag cgagcacgat ctgcggtgac agggccggcc gccagctctg gggcggcacg
      481 ttccacgcgg tcgcgcacaa gcttgtctcc gcccacgccg agacactcgg gctcccaccc
      541 gcactgtcgg tgctcgatcc ggctgacgcg cgcgacctca tggatctgct gcgatccgac
      601 catcacttgt cgggtgcgga gcaacgactc ccccgcggtg acacgttggt tgacatctat
      661 tcgcgtgcgg tcaacacgtc gcagccggcg cgcgacgtaa ttgccagcag cttcccgtgg
      721 tgcgatcccc acaccgagtc gattctcgaa ctgtttcgcg cgtacgtggc acgcaagcgc
      781 gagcacggat ttctagactt cgacgaccta cttctcgcgt ggcgaagctt gctgaccgac
      841 ccgacactcg ggccaacgat cgccacccgc tgggattacg ttctggtcga tgagtaccag
      901 gacgtcaatc agatccaggt cgacatcgtc acgctgctaa gaccgggcgg ccaaggcctc
      961 actgtcgtcg gcgacgacgc gcaagccatc tacagtttcc gcggcaccga cagcaagcac
     1021 atcgtcgagc tctcacagac acttaccgat gcgcgagtcg ttcgactcga acgcaatttc
     1081 cgttctaggc aacgacttct cgacctcgcg aacgccgtcc gaccgagcac tgacggcgag
     1141 catctcgtgc ttcattccga gcgaacgggc gggcctcgcg cgcaactcgt gcgctgccac
     1201 gatgcagcat ccgaagctcg gctcgtcgtc gacgccgtcc tcgaaggagc ggagcaagga
     1261 gaacgtctgc gcgaccacgc cgtcctgatg cgcgcggcac atcacagcga tctcctcgaa
     1321 gtcgagctca cggcgcgtgg cgtcccgttt gtcaagtacg gcggactaaa gttcctcgaa
     1381 gcagcacacg tcaaagactt cgtcgcgggg ctacgtctgc tcgacaatcg gatcgatgag
     1441 atcgcgtggt tccggttact gcgattgcac gacggcatcg gtccggcgcg agcccgcgcg
     1501 ttgctcgact ccctgcaacc cggtgacgcc gaggcagaac aacgccatgc cgacgtcgct
     1561 gctatcgcgc cgcctggttc tcgggtcgct ctcaccgcga tgttggacgc cgtggtgtgt
     1621 gcgcggcagc agaaggctcc cgccgatcgg gccggggcag tactcgaatt tctgcggccg
     1681 ctgctgacaa atcgctacgc cgatcacgcc gcgcggctga ccgacctcga ccgccttgtc
     1741 ggcgcggcag ccaacgcgcc agacctcgcg gggttcgtcg ccgagctcac actcgacccg
     1801 ccggcgtcga cgaccgacct cgccggacca ccgcatctcg atgacgacta cctcgtgctc
     1861 tcgaccgttc actcggcgaa aggcctggag tgggagtccg tgcatgtcat tcacgtcgtc
     1921 gacggcgcgt tcccctccga cctggcactg tcgacaaaga ccggattgct tgaggaacaa
     1981 cggttgttct acgtcgcagt cactcgggcc cgtgatcgcc tctcgatcta cacaccgctg
     2041 cgcatgccgc accatcggtt tgcgcgtgac gacctacaca gctatgcgct cgggagccga
     2101 ttcctcgacg atgcggcgct ggccgccctc gacgtccagg aaagtgttgt gacgaggccg
     2161 tcgatcgtgg cgctgccggc aaacgcacga gtcgccatgc cggcgctcga cgagctgtgg
     2221 acttagctgg gtatttggct cagtggcgcg acgagagtcc gggattgccg ggtgtagcag
     2281 tgcgatccgc acgtcacgca ggtgaaagcg ttctggtcct cgacgccggg tgatccgcag
     2341 tcgaagcacg acatcgcgcc gcgtggcatt gggggtagag cgtcggcgtc agtacgcggg
     2401 gcgaccaact gccgcacgac ctcgtcgagg gcctgcgcta ccgcaatctc gcactcggcg
     2461 cagcgcgcac gtcgcaccgt gacgccgctg cccgcaggcg ttggcgtctc ccggccgcat
     2521 gaatcgcagg gcacagtcgg ctcgggatac gactcggtcg aagatgtctg ggtgctcacg
     2581 cctttggtat cggcgtgagc gctcttaggt gaacggccac ctgcgcggcg aacgggtcta
     2641 agtgtcgact gtttggccca acaggcggcg cgacacacgg gacatacccg aggtcctttg
     2701 cacagaccgc ccacgatggg acagtgacca tgaggcgcct ggtttctgct cccaggtagt
     2761 gccaggcgcc gaactatgct ccgtggcgcc gctccgctcc ttcgcgaaaa tgcgatggcg
     2821 gacgccgata gcgtgaagcg tctggatcag cggccgagag agtgcgcaca gtaatgacgg
     2881 ttccccgttt cacaccacgc gttgatgccc ggccggtcga cacggcatgg ccgcgattgc
     2941 agtggccacc ggccgccgac gtgcgactgc gcggtc
//
LOCUS       DASWVF010000576         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_271099, whole genome shotgun sequence.
ACCESSION   DASWVF010000576 DASWVF010000000
VERSION     DASWVF010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271099"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGD55_10140"
     CDS             <1..307
                     /locus_tag="VGD55_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870745.1"
                     /translation="SAASYAMGLILKDEDAFAPFIQGVSLPLLLLSGVLLPMSLAPSW
                     LRHLSQINPLTHVVDATRALFRGDFGNGDVLWGIAVTIALAALLAAWGTRTFQRQSA"
     gene            complement(372..1685)
                     /locus_tag="VGD55_10145"
     CDS             complement(372..1685)
                     /locus_tag="VGD55_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0870746.1"
                     /translation="MSSSVTPLPHAAPAPPERTMPTEVRDALSWLAHVLDESLPLRST
                     EDTSDFAHRRLNRGGIGLITLHEQLGPLTAGTAAHAFGNDMRRFPPVEVAGLYSSESP
                     RWKLLDLADDIKSVPSGLVAAFAAGTLAEADLVVSISIDGQYKAVNVNARAEDAKLAQ
                     AYLNDLRRRSREADSYLRGRCLRVRGDGESINVTPMKAPTAKRSDVIVSEAVWRELDL
                     NVSALFARRELLTKLRLGTNRGMLLYGPPGTGKTALCRVLAAELVGDVTVVLCDARAI
                     GVRMGEVYQELSRLAPALIVLEDLDLVVGNRRLGRSGGLHDFLAALDGVMSNHDGIVT
                     VATTNDIKALDDAAVRTARFDRIIEVPLPDAGLRALILTRYLGSLASAIDVRAVAAAT
                     DGASGAVLRELVRRAVLVEGDAFTTATLLRVARDGLGATTTGQYL"
     gene            1898..>2862
                     /locus_tag="VGD55_10150"
     CDS             1898..>2862
                     /locus_tag="VGD55_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0870747.1"
                     /translation="MSILNGRATTIYDVARMAGVSKSTASRVLTGDRRVSAEAVEQVS
                     HAAAALGYVPNPAALSMSTGKGTRVVIGVVSPTARLVVDDYLARVVATAAKVCAPERI
                     GVGLEALALDGADPLDVLARDPTVRGVVLVNTTDSVLRAVDHRLAGRIVSIGVGSPLI
                     PSVDVDNHTAAREITCRLVESGRRRIAMVSGPRWLPCVDRMVQGYGEVMRDAGLQRRV
                     VAGGFKAEDGRTATASVLRRWPDIDAIFAVCDDTAMGVLAQLRARHIGVPSDVAVAGF
                     DDTALAQFAGLTTATHPVETIAEAATRAVLRASAARPPDMYFASHL"
BASE COUNT          455 a         1008 c          944 g          455 t
ORIGIN      
        1 gtcggcggcg tcgtatgcga tgggtctgat cctgaaagac gaggacgcgt tcgcgccgtt
       61 catccagggc gtctcgctgc ccctgctgtt gctgtcggga gtgctgctcc cgatgtcgct
      121 cgcgccgagt tggctgcgac acctgtcgca gatcaatccg ctcacgcatg tggttgatgc
      181 gacgcgggct ctgtttcgcg gcgacttcgg aaatggtgac gtgttgtggg ggatcgcggt
      241 gacgatcgcg ctcgctgcgc tactggcggc gtggggaacg cggaccttcc agcgccaaag
      301 cgcctaggcg agaagatcgg tcccgcctag gtcgcacgcg gcagcgacaa cgccgaccga
      361 ccggccagca cctacaggta ctggccggtc gtcgtcgcgc cgagcccatc gcgggcgacc
      421 cgcagcagcg tggccgtcgt gaacgcgtcg ccctcgacca gcaccgcgcg ccgcaccagc
      481 tcgcgcagca ccgcaccgct cgccccgtcg gtcgcggcag cgacggcacg gacgtcgatc
      541 gccgaagcca gcgaaccgag gtagcgcgtc aagatcaacg cgcgcaaccc ggcgtccggc
      601 aaaggaacct cgatgatgcg gtcgaaccga gccgtccgca ccgccgcgtc gtccaacgcc
      661 ttgatgtcgt tggtcgttgc gaccgtcacg atgccgtcgt gattgctcat gacgccgtcg
      721 agcgccgcca agaaatcgtg caggccaccc gagcgaccta gccgccgatt gccgacgacc
      781 aggtcgaggt cctcaagcac gatcagcgcg ggcgcgagcc tgctcagctc ctggtagacc
      841 tcgcccatgc ggacgccgat tgcgcgcgcg tcgcacaaca cgaccgtgac gtcaccgacg
      901 agctccgccg ccagcacccg acacaacgcg gtcttgccgg tacccggcgg accgtacaag
      961 agcatgccgc gattcgtgcc aagccgaagc ttggtcagca actcgcgccg cgcgaacaac
     1021 gccgacacgt tgaggtcgag ttcgcgccac actgcctcgc tcacgatgac gtccgaccgc
     1081 ttcgccgtcg gcgccttcat gggcgtgacg ttgatactct cgccgtcacc gcgtacccgc
     1141 aggcagcgac cacgcaggta gctgtcggcc tcacgcgacc gtcggcgcag atcgttcagg
     1201 tatgcctgcg cgagcttcgc gtcctctgcc cgcgcgttca cattgacggc cttgtactgc
     1261 ccgtcgatgc tgatcgacac cacaagatcg gcctcggcca acgtccccgc tgcaaatgca
     1321 gcaaccagcc cgctcggcac gctcttgatg tcgtcggcaa gatcgagcag cttccacctc
     1381 gggctctcac tcgagtacag accggcgacc tcgaccggcg ggaaccgacg catgtcgttg
     1441 ccgaacgcgt gcgccgcggt acctgcggtc aacggcccaa gctgctcgtg caacgtgatc
     1501 aacccgatgc cgccgcgatt caagcggcgg tgcgcgaaat cgctggtgtc ttcggtcgag
     1561 cgcaacggca gactctcgtc aagcacgtgc gccaaccacg acaacgcgtc acgaacctcg
     1621 gtgggcatcg tgcgctcggg cggagccggt gccgcgtgcg gcaaaggcgt aactgacgac
     1681 gacatcggac tactcaactc cttacgcgcg cgacggggca ctcccgtcgg ctaggcgcgc
     1741 aggagtgcca tccgtgatga ccatggagta cgacatgact gcacgagggt aggggcatct
     1801 cacgagggcg gccagtcagt ttccgtactg aatcggttga cggcggctcg cgcgcgctga
     1861 ttggctggcg ccgtaaccgg ttccggaggt gcagggcgtg tcgattctca acggccgcgc
     1921 gaccacgatc tacgacgtcg cgcgcatggc gggcgtttcg aagtcgactg cctccagggt
     1981 tttgacgggc gaccggcggg tgtcggccga agccgtcgag caggtgagcc acgccgctgc
     2041 cgcgctgggc tatgtgccca acccggcggc gctctcgatg tcgacaggca aaggcacccg
     2101 cgtggtgatc ggcgtcgtct cgccgaccgc gcggttggtc gtcgacgact acctcgctcg
     2161 cgtggtcgcc acggccgcca aggtgtgcgc acctgagcgc atcggtgtcg gcctcgaggc
     2221 gctggcactc gacggcgctg acccactcga cgtcttggct cgcgacccga ctgtgcgcgg
     2281 cgtcgtcctg gtcaacacca ccgattcggt tctgcgcgcg gtcgaccacc gcctcgccgg
     2341 gcgcatcgtg tcgatcggcg tcggctcgcc gctgatcccc tcggtcgatg tcgacaacca
     2401 cacggccgcg cgcgaaatca cctgccgcct ggtcgagtcg ggccgccgcc gcatcgcgat
     2461 ggtcagcggc ccgcgctggt tgccgtgcgt cgaccgcatg gtgcagggct acggggaggt
     2521 catgcgcgac gcgggattgc aacgccgcgt tgtcgccggc ggattcaaag cagaagacgg
     2581 ccggacggcg acagctagcg tcctgcgccg ctggcccgac atcgacgcga tcttcgctgt
     2641 ctgtgacgac accgcgatgg gcgtgctggc gcagttgcgg gcccggcaca tcggcgtccc
     2701 cagtgatgtc gccgtcgctg gattcgacga caccgccctc gcgcagttcg cggggctcac
     2761 caccgcgacg catccggtcg agacgatcgc cgaggccgcg acccgtgcgg tgctgagggc
     2821 cagcgccgcc cggccgcccg acatgtactt cgcgtcgcac ct
//
LOCUS       DASWVF010000577         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_271130, whole genome shotgun sequence.
ACCESSION   DASWVF010000577 DASWVF010000000
VERSION     DASWVF010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271130"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGD55_10155"
     CDS             <1..268
                     /locus_tag="VGD55_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870748.1"
                     /translation="TDRDTLSRLVKAGRHARGLHFQLVSVRTVAQSPGSVTLAVADTL
                     AGYDVVGPTGSAEPVAGRGLRTWTVVLRAQPLSGPWRIASIDAA"
     gene            324..>1500
                     /locus_tag="VGD55_10160"
     CDS             324..>1500
                     /locus_tag="VGD55_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870749.1"
                     /translation="MVKPRIVGVLVFAGALVACGSVRTGPATGRGSIAALSSIAGPSA
                     APSSAPLASSAPPASVPPASIPATGSPFPAGSELGIPQAEMESDAEVLYAQNGVTLVA
                     ITPGCCGSQEGKPSTLWMTTDLRHWRDVTPPGSRKLVNAGYFPGMYAGFDEASFLNPT
                     TGWVTTWNSGNLAVIAYRTSDGGKTWTSVGMSGHGDHAGDADWIQLLTPKVAFDENVA
                     ATAPNFSLAMTSDAGRSWHDIYRWPSPTNDLAPPPMPFAMPIFFVTATRGFAASGIPP
                     AEGDVTGDFFATSDGGVTWSRLTPPSIKNGACPAVQTYPLTVQCLVSLPRFADSTNGV
                     LATEVIDGANASVGFDVTRDAGTSWHLATSVDVALPVFPSGGYPTTNYAFVSMPSITT
                     "
BASE COUNT          222 a          500 c          507 g          271 t
ORIGIN      
        1 cactgaccgc gacacgttga gtcgactggt gaaggcgggt cggcacgcgc gcgggctgca
       61 tttccagctg gtgtcggtgc gaacggtcgc gcagtcacca ggctcggtga cgctcgcggt
      121 cgccgatacg ctcgccggct acgacgtcgt cgggccgacg ggttcggccg agccggtcgc
      181 cggtcgcggc ctgcgcacgt ggaccgtcgt cttgcgcgct cagccattgt cgggtccgtg
      241 gcgcatcgcc tcgatcgacg cagcctgacc tcaccagtcg gtccgcgatc caaccgatcg
      301 cccgcgtcgg gcgtcctgcc gttgtggtga aaccgcggat cgttggtgtg ctcgtgttcg
      361 ccggggcgtt ggttgcctgc gggagcgtta ggactggacc ggccaccggt cgcggatcga
      421 ttgcggcgct gtcctcgatc gccgggccgt cggccgcgcc ctcgtcggcg ccactggcat
      481 cgagtgcgcc tccggcgtcg gtgccaccag catcgatccc tgcaactggc tcgccgttcc
      541 cggctggctc cgagctcgga attccccaag ccgagatgga gtccgacgcc gaggttcttt
      601 acgcgcagaa cggggtcact ctcgtcgcca tcacgcccgg ctgttgtggg tcgcaggagg
      661 gcaaaccgtc gacgctttgg atgaccaccg acctgaggca ctggcgcgac gtcactccgc
      721 cggggtctcg caagctggtg aatgccgggt acttccccgg gatgtatgcg ggattcgacg
      781 aggcctcatt cctcaacccg accaccggct gggtcacgac gtggaacagc ggcaacctcg
      841 cggtcattgc gtaccgcact agcgatggcg gcaagacctg gacttcggtc gggatgagtg
      901 ggcatggcga ccacgctggc gacgccgatt ggatccagct actgacaccg aaggtcgcgt
      961 ttgatgagaa cgtcgcggct acggcgccga acttcagtct tgcgatgacc tcggacgccg
     1021 gccggtcgtg gcacgacatc taccgatggc cgtcaccgac gaacgatctg gcgccaccgc
     1081 cgatgccgtt cgcgatgccg atcttcttcg tcacggcgac ccgggggttc gcggcttccg
     1141 gcattccgcc cgccgagggc gacgtcaccg gggacttctt cgccaccagc gacggcggcg
     1201 taacgtggtc gcggctgacc ccgccgtcca tcaagaatgg agcttgcccg gcggtgcaga
     1261 catacccgct gactgtccag tgcctcgtgt cgttgccgag gttcgccgac tcgacgaacg
     1321 gagtgttggc gactgaggtg atcgacggcg cgaacgcgag tgtcggattc gacgtcacgc
     1381 gcgacgccgg cacgagttgg cacctggcga cgtcggtcga tgtggccctg ccggtgtttc
     1441 caagtggcgg atatccaacg accaactacg cgttcgtctc gatgccgtcg atcaccacat
//
LOCUS       DASWVF010000578         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_474952, whole genome shotgun sequence.
ACCESSION   DASWVF010000578 DASWVF010000000
VERSION     DASWVF010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474952"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..>1512)
                     /locus_tag="VGD55_10165"
     CDS             complement(87..>1512)
                     /locus_tag="VGD55_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0870750.1"
                     /translation="TLYSVLSHMATPDKNVVTLEDPVEIQLPGITQVQTNERAGLTFS
                     KGLRSILRQDPDVVLVGEVRDSETAELALQASMTGHLVLTTLHTNDAVAALTRLVDMG
                     IQPFLIASSLTLVIAQRLVRRPCAECVAPYIPSARVLTLLGLTEADLVGATPMRGRGC
                     GECGGTGYRGRTGIFEVLPVTASLRSVLSHTPTEAAVGAAARAAGMTTLRGSGLARAR
                     RGETTYEEILRATQVDAIEGQRCSACDRAVDDDMIACPWCETIVDRGHCTACARSLEP
                     GWKICPWCRTAARETATAVGSGVRLPRLLVVGDDGAVPNLIRSAAGTAMDVDTAATAD
                     DALTAVWEGDYDGVIVDQSLPGVGGVELLRLMRTGSRTAALPLMLVGKFDEDSRDSHV
                     GGIDEVVALSASPIEMMTRLTLLTERSPHASAFNGAGVRAGAAAIDQPVVSDEKPAPA
                     NRRVRSVPVASERRSRQSANRPKS"
BASE COUNT          252 a          503 c          510 g          247 t
ORIGIN      
        1 ttgaggtctg ccacgcgcgt gcatctgccg ggcatcgaag ctcacttcgc ccgtcgcttt
       61 actcggcagg agctcgcccg gctcggtcag ctcttgggtc ggttggccga ctgacggctt
      121 ctgcgctcgg aggccaccgg caccgaacgc acccgtcgat tggccggtgc gggcttctcg
      181 tcggagacga cgggctggtc gatggcggcg gcgccagcgc gcactcccgc gccgttgaaa
      241 gctgacgcgt gtggactgcg ctcggtcaac agcgtcaggc gcgtcatcat ctcgatgggc
      301 gatgcgctga gcgcgacgac ctcgtcgatg ccacccacgt gggaatcacg ggaatcctca
      361 tcgaacttcc cgaccagcat caacggcagt gccgcagttc ggctgccggt acgcatcaga
      421 cgcaacagct ccaccccgcc gacgccgggc aggctttggt cgacgatcac gccgtcgtag
      481 tcgccctccc acaccgcggt gagggcgtcg tcggcggtgg cggccgtgtc gacatccatc
      541 gcagtacccg ctgccgatcg gatcaggttg gggaccgcgc catcgtcacc taccacaagc
      601 aaacgcggta agcggacgcc ggagccaacg gccgttgcgg tctctcgtgc ggcggtacgg
      661 caccacggac agatcttcca accaggctcg agcgaccgcg cgcaggctgt gcagtgccca
      721 cggtcgacga tggtctcgca ccacgggcaa gcgatcatgt cgtcgtcgac tgcgcggtcg
      781 caggcggaac accgttggcc ttcgatcgcg tcgacctgcg tagcgcgaag gatctcctca
      841 tacgtcgtct cgccacgtcg ggcacgggcc aggcccgagc cgcgcaacgt cgtcatgccg
      901 gcggcgcgtg cggcagcccc gactgccgcc tcggtgggtg tgtgtgacag cacggaccgc
      961 aagctggcag tgaccggcag gacctcgaag attccggtgc gaccgcgata cccggtgcca
     1021 ccgcactcgc cacagccgcg accacgcatc ggggtggcgc cgacgaggtc ggcttccgtg
     1081 agaccgagca gcgtaaggac gcgggcgctc ggtatgtagg gcgcgacgca ctcggcacaa
     1141 gggcggcgaa ccagtcgctg tgcgatgacc agggtcaacg atgatgcaat caggaacggc
     1201 tgaatgccca tgtcgaccag tcgcgtcaat gcagccactg cgtcgttggt gtgcaaggtc
     1261 gtgagaacga ggtgcccggt catcgacgcc tgcaacgcga gttcggctgt ctcggagtcg
     1321 cggacctcac cgacgagaac cacgtcgggg tcttggcgca ggatcgaccg caagcccttg
     1381 ctgaacgtga gacctgcgcg ttcgttcgtc tgcacctgcg tgatgccggg caactggatc
     1441 tcgaccgggt cttcgagcgt cacgacgttc ttgtccggcg ttgccatgtg cgacagcacg
     1501 gagtagaggg tg
//
LOCUS       DASWVF010000579         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_500396, whole genome shotgun sequence.
ACCESSION   DASWVF010000579 DASWVF010000000
VERSION     DASWVF010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500396"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..886)
                     /locus_tag="VGD55_10170"
     CDS             complement(26..886)
                     /locus_tag="VGD55_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY0870751.1"
                     /translation="MTAEPEVLRYTAFTDTPDGGNPAGVVLDATGLDADTMQAIAKEV
                     GYSETAFLTPAADDGRTFNIRYFAPSIEVPFCGHATIATGVVLGERLGEGLFLLHAKS
                     GDVPVDVDKDDNGVRATLTSVEPDLKDLDAADLDALLEARRWGHDDLDPTYPPKVAYA
                     GIWHPVLAAKTRARLADLDYDFETLRTLMTDLGWTTIQLVWREAPTIFHVRDPFAAGG
                     VVEDPATGAAAAALGHYLRETGLIDPPFTLTLHQGVDMGRPSLLIVHVDDADPRIRVG
                     GHAVAILEAT"
     gene            complement(900..1379)
                     /locus_tag="VGD55_10175"
     CDS             complement(900..1379)
                     /locus_tag="VGD55_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0870752.1"
                     /translation="MTETNASSDPMRSAIDVEFVRPEILYVLRRDVLRPGQSLSAVKH
                     EYDFQPDTFHVAAFGPRNQVAACASFYPEPPPDKAAENAWRLRAMASRPELRGQGYGA
                     AALRYGIAEIRRRGGTLLWCNARTGAVGFYERIGFSIVSDEFEVESIGPHFVMEIHL"
     gene            complement(1390..>1785)
                     /locus_tag="VGD55_10180"
     CDS             complement(1390..>1785)
                     /locus_tag="VGD55_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0870753.1"
                     /translation="LKNLTSQLIGRFCQSAEAATREAYGRGPLTRYDADLIVPRDSRI
                     ECALLKAVTAYYVMNREGVAAIHSKQRTLLLELVDRLTSQTGEGLEPLFRTAWAAAPG
                     DAAKLRVIVDQVASLTDTSAVIWHRRAAR"
BASE COUNT          308 a          556 c          595 g          326 t
ORIGIN      
        1 atccggccgg ccacgatcgt tgagtctaag tggcttccaa gatcgccacc gcgtgcccac
       61 cgacccggat gcgcggatcg gcgtcgtcca catgcacgat cagcaggctg ggccggccca
      121 tgtcgacgcc ctggtgaagc gtcagtgtga acggtgggtc gatcaggccg gtttcgcgaa
      181 ggtagtggcc aagcgcagcg gcggcagcgc cggtcgcggg atcctcgacc actcccccgg
      241 ccgcgaacgg gtcgcggacg tgaaagatcg tcggcgcctc acgccagacc agctgaatcg
      301 tggtccaccc gaggtctgtc atgagggtgc gaagcgtctc gaagtcgtag tcgagatcgg
      361 ctagccgggc gcgcgttttc gctgccagca ccgggtgcca gatgcctgcg tacgcaacct
      421 tcggagggta ggtcgggtcg agatcgtcgt ggccccatcg ccgcgcttcg agcagcgcat
      481 ctaggtcggc ggcgtcgaga tctttcagat cgggctcgac gctcgtcagc gtcgcgcgca
      541 caccgttgtc gtctttgtcg acgtcgaccg ggacgtcgcc ggatttcgcg tgcagcagaa
      601 agagcccttc gccgagtcgt tcgccgagca cgacaccggt cgcgatcgtc gcgtgcccgc
      661 agaacggcac ttcgatggac ggcgcgaagt accgaatatt gaacgtgcgg ccgtcatcag
      721 cggccggggt aagaaaagcc gtctccgaat agccgacttc tttggcgatc gcctgcatgg
      781 tgtcggcatc gagaccagtt gcgtcgagca cgacacccgc cgggtttccg ccgtcggggg
      841 tgtcggtgaa cgccgtgtac cggaggacct cgggctcggc ggtcattcgg gttcggccgt
      901 cataagtgga tctccatgac gaagtgtgga ccgatcgact cgacctcgaa ttcgtcgctc
      961 acgatggaaa atccaatccg ctcgtagaag ccaaccgcgc ctgttcgggc attgcaccac
     1021 agcagcgtgc cgccgcgcct gcgaatttcc gcgatgccat aacgcaaggc ggcagccccg
     1081 taaccctggc cgcgcagttc gggccgggag gccatggctc gcagccgcca cgcgttctcc
     1141 gcggctttgt ctggcggtgg ctcaggatag aacgatgcgc aggccgctac ttgattgcgc
     1201 ggaccaaatg cagcgacgtg gaaggtgtcg ggctggaagt cgtactcgtg cttgaccgca
     1261 ctcagtgact ggcccggcct caagacatca cgcctgagca cgtagaggat ctcgggtcgg
     1321 acgaactcga catcgatggc gcttcgcatc gggtcggagc ttgcgttggt ttcggtcacg
     1381 gtctgcttct caccgggcgg ctcgtcgatg ccagatcact gccgatgtgt cagtcagcga
     1441 ggcgacctgg tcgacaatca cccgcagttt ggccgcatcg ccaggagcgg ccgcccacgc
     1501 cgtccggaaa agaggttcga gtccttctcc ggtctgcgat gtgagccggt cgacgagttc
     1561 gagcaacaag gtgcgctgct tgctgtgaat cgccgcgacg ccctcgcgat tcatgacgta
     1621 atacgcggtg acagccttga gtagcgcgca ctcgatgcgg ctgtcgcgag gcacgatgag
     1681 gtcggcgtcg tagcgagtca ggggtccacg accgtaggcc tcacgagtgg ccgcctcggc
     1741 tgactggcag aacctgccga tgagctggct ggtgagattc ttcaa
//
LOCUS       DASWVF010000580         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_41653, whole genome shotgun sequence.
ACCESSION   DASWVF010000580 DASWVF010000000
VERSION     DASWVF010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41653"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGD55_10185"
     CDS             complement(<1..619)
                     /locus_tag="VGD55_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0870754.1"
                     /translation="MTSDVGRRSVGADETAQANRRWWDTAADDYQAEHGEFLGGPDQA
                     RFIWGPEGLDEASAQLLGPTAALAGRRVLEIGCGAASCARWLAAQGAFTVAFDLSARQ
                     LRHAGVPNGSVRLLQADAQRIPISDAGIDIACSAYGALPFVADSAAVLGEVYRVLQPG
                     GRFVFSVTHPIRWALPDDPGPGGLTISSSYFDRTPYVESDADGRAT"
     gene            861..>2291
                     /gene="rpsA"
                     /locus_tag="VGD55_10190"
     CDS             861..>2291
                     /gene="rpsA"
                     /locus_tag="VGD55_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY0870755.1"
                     /translation="MTSSTYVRPETVLGAAPHISPQVAVNDIGSSEDFLAAIDETIKY
                     FNDGDIVDGTIVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPNDIVKVGDHIEALV
                     LQKEDKEGRLILSKKRAQYERAWGTIEQIKENDDIVKGTVIEVVKGGLILDIGLRGFL
                     PASLVEMRRVRDLQPYVGRELEAKIIELDKNRNNVVLSRRAWLEQTQSEVRQTFLTTL
                     KKGQIRNGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGMEVTVEVLD
                     VDMDRERVSLSLKSTQEDPWQAFARTHAIGQVVPGKVTKLVPFGAFVRVEEGIEGLVH
                     ISELAERHVEIPEQVVQVGDEIFVKVIDIDLERRRISLSLKQANESSGDTAVADTHFD
                     PTLYGMAANYDAEGNYIYPEGFDSETNEWLPGHEEKQAEWERQYAEARARFEAHQSQI
                     VEAQKAEAAAGTEDDAETSATSSTTTS"
BASE COUNT          427 a          760 c          753 g          351 t
ORIGIN      
        1 aggtcgcccg cccatcggcg tccgactcga cgtagggggt gcggtcgaag tacgacgagc
       61 tgatcgtgag tccgcccggc ccgggatcgt cgggcaaggc ccagcggatc gggtgcgtca
      121 ccgagaagac gaaccgaccg ccgggttgca agacgcggta gacctcgccg agcactgcgg
      181 cggagtcggc gacgaagggg agcgcgccgt aggcgctgca cgcgatgtcg atacccgcgt
      241 cgctgatcgg gatgcgctgg gcgtccgcct gtaggagacg gaccgatccg ttcggcaccc
      301 ccgcatgccg gagctggcga gctgacaggt cgaaggcgac cgtgaaggcc ccctgtgcgg
      361 cgagccaccg ggcacacgac gcggcgccgc agccgatctc cagcacccgg cgcccggcga
      421 gcgcagcggt cgggcccaga agttgagcag aagcctcgtc aagcccctcc ggaccccaga
      481 tgaagcgcgc ctggtcgggg ccaccgagga actcgccgtg ctcggcttgg tagtcgtcgg
      541 cagcggtgtc ccaccagcgc cggttggcct gcgccgtctc gtcggcgccc actgaccggc
      601 gtccgacatc gcttgtcaca ctcgcccctt tctgcatgat cacggtccgg attgaccggt
      661 tcccagcgtg ccacgtaagg tggttggtgc gtgtgggcct gcgtgcgcct cggtatggag
      721 caggctcgcg atcgcttcgg tcgacctcgg taacccctcc cgcgcttaac tgccgcagtg
      781 tggccgccgg tacagcggcc gaaacgacgg gcccgtacgc gcctgccgac ctgttgtccg
      841 cgaccggagc cgactccctt atgaccagca gcacctacgt acgacccgag accgtcctcg
      901 gagcagcacc gcacatctcg ccccaagtgg ccgtcaatga catcggatcc tccgaggact
      961 tcctcgctgc gatcgacgag acgatcaagt atttcaacga cggagacatc gtcgacggca
     1021 cgatcgtgaa ggtcgaccgt gacgaagtgt tgcttgacat cggctacaag accgagggcg
     1081 tcattccctc ccgcgagctg tcgatcaagc acgacgtcga tcccaacgac atcgtcaaag
     1141 tgggcgacca catcgaggcc ctggttctcc agaaggagga caaggagggt cggctgatcc
     1201 tgtccaagaa gcgcgcacag tacgagcgcg cttggggcac gatcgagcag atcaaggaaa
     1261 acgacgacat cgtcaagggc accgtcatcg aggtcgtcaa gggcggcctg atcctcgaca
     1321 tcggtctgcg cggcttcttg ccggcgtccc tcgtcgaaat gcgtcgcgtc cgcgacctcc
     1381 agccctacgt cggccgcgag ctcgaggcga agatcatcga gctcgacaag aaccgcaaca
     1441 acgtcgtgct ctcgcgccgt gcgtggctcg agcagaccca gtccgaggtg cggcagacct
     1501 tcctcaccac gttgaagaag ggtcagatcc gcaacggcgt cgtcagctcg atcgtcaact
     1561 tcggtgcgtt cgtcgacctc ggcggcgtcg acggtctcgt ccacgtctcc gagctgtcgt
     1621 ggaagcacat cgaccacccg agtgaagtgg tcgaggttgg catggaggtc acggtcgagg
     1681 tgttggacgt cgatatggac cgtgagcgcg tctcgctctc gctcaagtcg acccaggaag
     1741 acccctggca ggcattcgcc cgcacccacg cgatcggcca ggtcgtgccg ggcaaggtca
     1801 ccaagctggt tccgttcggc gcattcgtgc gggtcgaaga gggcatcgag gggctcgtgc
     1861 acatctcgga gctggccgag cgccacgtcg agatccccga gcaggtcgtg caggtcggcg
     1921 acgagatctt cgtcaaggtc atcgatatcg acctcgagcg ccgtcgcatc agcttgtcgc
     1981 tgaagcaggc caacgagagc agcggcgaca ccgcggttgc cgacacgcac ttcgacccca
     2041 cgctgtacgg catggcggcg aactacgacg ccgagggcaa ctacatctac cccgagggct
     2101 tcgactccga gaccaacgaa tggttgccgg gtcatgagga gaagcaggcc gagtgggaac
     2161 ggcagtacgc cgaggcccgg gcgcgcttcg aagcgcacca gagccagatc gtcgaggcgc
     2221 agaaggccga ggcggcagcg ggcacggaag acgacgctga gacctccgcc acttcctcca
     2281 caaccacctc g
//
LOCUS       DASWVF010000581         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_500504, whole genome shotgun sequence.
ACCESSION   DASWVF010000581 DASWVF010000000
VERSION     DASWVF010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500504"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="araB"
                     /locus_tag="VGD55_10195"
     CDS             <1..1438
                     /gene="araB"
                     /locus_tag="VGD55_10195"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY0870756.1"
                     /translation="PAAVASAGISASDVVGIATDFTACTVLPTRADGTPLCMAPEFAD
                     EPHAWVKLWKHHAAQGQADRINELARLRGEPWLPRYGGKISAEWEFAKALQVLEEAPA
                     VYAATDRWIEAADWIIWQLCGVETRNACTAGYKGIFQDGQYPSADYLAELNPGFAKFV
                     VDKLEFPLSPLGGRAGSLTEEAAAWTGLPAGIAVAVGNVDAHVTAPAAQAVAAGQMVA
                     IMGTSTCHVVNAGSLAEVPGMCGVVDGGIIDGFFGYEAGQSGVGDIYAWFADDIVGAN
                     VTDAARAAGQNVHEYLSALAAEQEVGHHGLVALDWVNGNRSVLVDAGLSGVIVGLTLA
                     TKPEDIYRALVEATAFGTRLILSTFESSGIAISEFIAAGGLIKNNFVMQTYADVLARP
                     ISVIDTDQGPALGSAIHAAVAAGEYPDVPTASAAMGKKRTHVFLPNLAASQTYDLLYA
                     EYVRLHDYFGRGENDVLHRLRAIRNAHR"
     gene            1435..2124
                     /locus_tag="VGD55_10200"
     CDS             1435..2124
                     /locus_tag="VGD55_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEY0870757.1"
                     /translation="MTGHAPAGVDPAAFDAVCREVADLHAELIRWGLVVWTSGNVSAR
                     ISGTDCIVIKPSGVSYADITPESLVACDLDGQQIYGNGSPSSDTAAHAFVYQQLPDVG
                     GVVHTHSTYATAWAARGEAIPCVLTAMADEFGGEIPIGPFALIGDHSIGRGIVDTLRG
                     RRSPAVLMKNHGVFTIGKTPRDAVKAAVMCEDVARTVHIARQLGEPLSIEPADIDRLY
                     DRYTNAYGQQP"
     gene            2153..3667
                     /gene="araA"
                     /locus_tag="VGD55_10205"
     CDS             2153..3667
                     /gene="araA"
                     /locus_tag="VGD55_10205"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY0870758.1"
                     /translation="MPDSNEKLECWFLTGSQGLYGAQTLRQVDQQSSEIVSTLNAGPE
                     IGVRIVWKPVLTDADAIRRACIDASSDDNCIGVVAWMHTFSPAKAWIAGLDILRKPLL
                     HLHTQANLSLPWASIDMDFMNLNQAAHGDREFGYIETRMRIPRKTVAGHAKDPAVRSR
                     IDSWARAAAGFHAARTMRLARFGDNMRDVAVTEGDKVEAQLRLGVSVNTYGVNDLVAA
                     VDAADARSIDETVAQYEHEYTIVPELAKTGDRHESLRYAARIEVGLRSFLEQGAFTAF
                     TTNFEDLGGLRQLPGIAVQRLMADGFGFGGEGDWKTAAMVRIMKVIAGNRPGGTSFME
                     DYTYHFGPGEPKILGAHMLEVCPSIATGRPSCEIHPLGIGGREDPVRLVFDAAPGPGF
                     VVGLIDLGDRFRMVANDIEIVAPDEPLPKLPVARAVWKPAPTLSTSAESWLTAGGPHH
                     TVLSTAVGTDALEDFAVMTDIELVRIDADTTTSRFARELRWNQAYYRLAQGLSR"
     gene            3687..4028
                     /locus_tag="VGD55_10210"
     CDS             3687..4028
                     /locus_tag="VGD55_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870759.1"
                     /translation="MLDKKLQLKDGHTIALIGDPVQLPLEAHPAGLETADAVLVFAVN
                     HEKLQENLAALQSAARQGKLTWVAYPKAKQLDTDLDRDIIRDMVNQNGLDPVRQIALD
                     DIWSALRLKAL"
BASE COUNT          770 a         1297 c         1345 g          754 t
ORIGIN      
        1 accagcagca gttgcgtcag ctggcatctc ggcgagcgac gtcgtcggga tcgcgaccga
       61 cttcacggcc tgcactgtgt tgccgaccag ggcagacggc actccgttgt gcatggcgcc
      121 ggagttcgcg gacgaaccgc atgcgtgggt gaagttgtgg aagcaccacg cggcgcaagg
      181 tcaagccgat cgcatcaacg aactcgcgcg gctgcgtggc gaaccttggc taccgcgcta
      241 tggcgggaag atctcagcgg aatgggaatt cgcgaaggcg ttacaggtgc ttgaagaggc
      301 gcccgctgtg tatgccgcca ccgatcgctg gatcgaggcg gccgactgga tcatctggca
      361 gctgtgtggc gtcgagacga gaaacgcttg cacggccggc tacaagggga tcttccagga
      421 cgggcagtat ccgtctgctg actacttggc cgagctcaac ccgggctttg cgaaattcgt
      481 ggtcgacaag ctcgagtttc cgctgtcacc gctcggcggt cgcgctggct cactgaccga
      541 agaggccgct gcatggaccg gcctacccgc aggcattgcg gtggccgtcg gcaacgtaga
      601 cgcgcacgtg accgcgcctg cggcgcaggc cgtcgctgcc gggcaaatgg tcgccatcat
      661 gggaacctcg acgtgtcacg tcgtcaacgc cggatcgctc gcggaggtgc caggcatgtg
      721 tggcgtcgtc gatggcggaa tcatcgacgg cttcttcggc tacgaagccg gccagagcgg
      781 cgtcggggat atctatgcgt ggttcgccga cgacatcgtg ggcgctaacg ttaccgacgc
      841 ggcacgtgct gcgggccaga acgttcacga gtacctgagc gcactcgcgg ccgagcaaga
      901 ggttggccac cacggcctcg tcgcgttgga ctgggtcaac ggcaaccggt cggtgctcgt
      961 tgacgcaggt ctttctggcg tcatcgtggg cctgacgttg gcgacgaaac cagaagacat
     1021 ctaccgagcc ctcgtcgagg ctacggcgtt cgggacgcgg ctgatcctgt cgacctttga
     1081 aagctcgggt atcgcgatct cggagttcat cgctgccggg ggcttgatca agaacaactt
     1141 cgtgatgcag acctacgccg acgtgcttgc gcgcccgatc agcgtcatcg ataccgatca
     1201 gggcccagct ctcggctcgg ccattcatgc agccgtcgcg gctggcgaat atcccgacgt
     1261 ccccacggcg tcggcggcga tgggaaagaa gcgcacgcac gtcttcctgc cgaatctggc
     1321 cgcctcgcaa acctacgatc tgttgtacgc cgagtacgtc aggctgcacg actacttcgg
     1381 ccgtggcgag aatgacgtcc tgcatcgcct gcgcgcgatc agaaatgcac accgatgaca
     1441 ggtcacgcac cggcgggtgt agatccggcg gcattcgacg cggtatgccg cgaggtcgct
     1501 gatctgcatg cggagttgat tcggtggggc ctggtggtgt ggacgtcggg caacgtctcg
     1561 gcacgcattt cgggaaccga ttgcatcgtc atcaagccga gtggcgtttc gtatgccgat
     1621 atcacgccgg aatcactggt cgcctgtgat ctcgatgggc agcagattta cggcaacgga
     1681 tcgccgtcca gcgatacggc ggcgcacgct ttcgtctacc agcagttacc cgatgtcggc
     1741 ggcgttgtgc atacgcactc gacctatgcc accgcctggg ccgccagagg cgaagcgatc
     1801 ccgtgcgtgc ttactgctat ggccgatgag ttcggcggcg agattccgat cggcccgttc
     1861 gcattgatcg gtgaccactc gatcggtcgg ggcattgtcg ataccttgcg cggacgccga
     1921 tcaccggccg tcctgatgaa aaatcatggt gtcttcacga tcgggaaaac accacgcgac
     1981 gcagtgaagg cagccgtgat gtgcgaagac gtcgcccgca cagtgcacat cgctaggcag
     2041 ctaggggagc ccttgagcat cgaacctgcc gacatcgacc ggctctacga ccgctacacg
     2101 aacgcctacg gacagcagcc atagcaagcc catgacaacc caggagcagt acgtgcccga
     2161 cagtaacgag aagcttgaat gctggttcct cacgggcagt caagggttgt acggagcgca
     2221 gaccctgcga caagtcgatc aacagtcgag cgagatcgtc tctacgctca acgcggggcc
     2281 agagatcggc gtccgcattg tgtggaagcc ggtgctgacc gatgccgacg cgatccggcg
     2341 cgcgtgcatc gacgcgagct ctgacgacaa ctgcatcggt gtcgtcgcgt ggatgcatac
     2401 gttctcgccg gcgaaagcgt ggatcgcagg gctagacatc ttgcgaaagc cgctgcttca
     2461 cctgcacacc caggcgaatc tttcgttgcc gtgggcgagc atcgacatgg atttcatgaa
     2521 tctgaaccag gccgcgcacg gcgaccgcga gttcggctac atcgagaccc gcatgcgcat
     2581 cccgcgtaag acggtcgccg gtcatgcaaa agatccggcg gtgcgctcgc gcatcgactc
     2641 gtgggctcga gcggctgcgg gcttccatgc tgctcgcacg atgcgactcg cacgattcgg
     2701 cgacaacatg cgcgacgtgg cggtcaccga gggcgacaag gtcgaggcgc aactgaggct
     2761 cggcgtttcg gtgaacacct acggcgtcaa cgacctcgtc gctgccgtcg atgcagccga
     2821 tgctcgcagt atcgatgaga cagttgcgca gtacgagcac gagtacacga tcgtgcccga
     2881 gctcgcgaag accggcgatc ggcacgagtc cttgcgttac gcggcgcgca tcgaggtcgg
     2941 attgcgcagc ttcctcgagc agggcgcgtt cactgcgttc accaccaact tcgaggatct
     3001 cggtgggttg cgacaactgc cggggatcgc ggtccagcga ctgatggccg acggcttcgg
     3061 cttcggcggc gagggtgact ggaagaccgc tgcgatggtg cgcatcatga aggtcatcgc
     3121 cggcaaccgc ccaggcggta cgtcgtttat ggaggactac acctaccact tcgggcccgg
     3181 cgagccgaag atccttggcg cgcacatgct cgaggtctgc ccgagcatcg ccacgggccg
     3241 gcccagttgc gagatccatc cactcggcat cggcggacgg gaagaccccg ttcgcctggt
     3301 gttcgatgcc gctcctggtc caggcttcgt cgtgggtctc atcgatctgg gtgaccgctt
     3361 ccggatggtc gcgaacgaca ttgaaatcgt cgcgccggac gagccgctgc cgaagctgcc
     3421 ggtcgcgcgt gcggtgtgga aacccgcacc gacattgagc acatcagcag agtcgtggtt
     3481 gaccgctggg ggtccgcacc acactgtgct tagcacggcg gtcggcaccg acgcactcga
     3541 ggactttgcc gtgatgactg atatcgagct ggtccgcatc gacgccgaca ccacgacgtc
     3601 ccgctttgct cgtgaactgc ggtggaacca ggcctactac cggcttgccc aaggcctctc
     3661 ccgctagcag ctgtaaagtc agcggtatgt tggataagaa gctccagctc aaagacggtc
     3721 acaccattgc gctcattggc gacccggtcc agttgcctct ggaggcacat cccgcaggcc
     3781 tggaaactgc cgatgccgta ctcgtgtttg cggtgaatca cgagaagctg caagaaaacc
     3841 tggcggcgct ccagtctgcc gctcgccagg gcaagctcac ctgggtggcc tatcccaagg
     3901 ccaagcagct cgacaccgac cttgaccgcg acatcatccg cgacatggtg aaccaaaacg
     3961 gactcgatcc ggtgcgccag attgcgctcg acgacatctg gtccgcgctg cggctcaaag
     4021 cgctttagcc acgccttcag ctgcgaggtt taggcgcgtc aggctcccca tcctgcctgg
     4081 gtgcccccgg ttcgctcgct cacgatgcgt tgggcgtatc cggaggccgc gtaggcggct
     4141 ctcgggtccg ggtgaaggcc catggc
//
LOCUS       DASWVF010000582         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_347519, whole genome shotgun sequence.
ACCESSION   DASWVF010000582 DASWVF010000000
VERSION     DASWVF010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347519"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VGD55_10215"
     CDS             complement(<1..422)
                     /locus_tag="VGD55_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0870760.1"
                     /translation="MKILAVLPHFEPDVAPTGVIASRLVEELGGLGHHVDVVTSLPWY
                     EHHAVLPAWRGRIVRREKTNWGSITRVHPFPTGDKLSIPKRAVGFAAFCALDAAVAAA
                     GAHVDVAFAMTPPLPMAATGWFAALARRAPLVLNVQDV"
     gene            complement(419..1567)
                     /locus_tag="VGD55_10220"
     CDS             complement(419..1567)
                     /locus_tag="VGD55_10220"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0870761.1"
                     /translation="MTLTVGVNLLWLVPGVVGGTESYATGLLERLVERDDVDVVAYAL
                     PDFVTTYPALATAAHTVVPPMPTGRHVVRRVLVENAWLPRHLRAQHIGLVHHLGGIVP
                     PGCRVPSVVTLHDLQYLAYPDYFSAAKRRYLSATQGSSLKRARVVMAVSEFTRTQAID
                     AFDLDADKVIVVPPVIRPLSAVSTQRRTAVLHELGVRGDFILNPAAAYPHKNHALLVN
                     AFAEVVKHHDVSLVLTGAAGAGAWGSAHSTRAEISDLVARLGLDRHVRMPGYVTAEQL
                     GVLYEEASMLAFPSRFEGFGIPVVEAMSVGCPVIVADATALPALVGDAGLLVDPDDVG
                     AWADAMNGLLDNPDARQRLADAGRARATELAAVDPVALVVDAYQRAAS"
     gene            complement(1564..2646)
                     /locus_tag="VGD55_10225"
     CDS             complement(1564..2646)
                     /locus_tag="VGD55_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0870762.1"
                     /translation="MRLRAAVTLEQCWHDVPGGTASSALETVKALQKRSDIEVLGVAA
                     RHDTLPPAPWTPTVNIYQLSLPRVALYESWHALRWPRVQAATGPIDVIHATTFAIPPK
                     SAPLVVTVHDLAFLHEPSHFTRHGRRFFTRGLALARTQADLVLVPSQSTLDDCVGAGF
                     DASRLRLVPHGVAVPDVSASQIAAFVSQHHLPERYVLWCGTLEPRKNVPTLLRAFAQV
                     RRSDPSLALVLVGPTGWGDVPTEAAGVHLLGFLPAEDLHAAYAGARAFCYPSLREGFG
                     LPVLEAMAHGVPVVTSSGTAMAEFVGDAGFLVDPLDADAMARGLEKAMGDRHDELAKA
                     GREKAAVYTWNKAAELTAEAYRQVAS"
     gene            complement(2643..3719)
                     /gene="gmd"
                     /locus_tag="VGD55_10230"
     CDS             complement(2643..3719)
                     /gene="gmd"
                     /locus_tag="VGD55_10230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0870763.1"
                     /translation="MAATTIPRSDAEATVSTSVSPSGKTALVTGITGQDGSYLAELLL
                     SKGYEVHGLIRRASTFNTERLDPIYQDPHDSDVRLRLHYGDLHDGVGLVNLMHAVEPD
                     EVYHLGAQSHVKVSFEMPDYTGDVTGMGTIRLLEAIRSSGISTRFYQASSSEMFGSTP
                     PPQNENTPFHPRSPYGAAKMYAYWVTVNYRESYGMFATNGILFNHESERRGETFVTRK
                     ITRAIARIQAGLQDKLYLGNLDAKRDWGYAPEYVEAMWQMLQVDEPDDFVIATGASYS
                     IQQFVEFAFERAGLDWRKYVETDPAYLRPAEVDDLCGDASKAKAALGWQATTHTPELA
                     RLMVDADIAMLADQLAGRTVRVDR"
BASE COUNT          680 a         1193 c         1249 g          604 t
ORIGIN      
        1 aaaacgtcct gcacgttcaa gacgagcggc gcccgtcgag ccagcgcggc gaaccagccc
       61 gtcgccgcca tcggcagcgg gggagtcatc gcgaacgcga catcgacgtg cgcgccagcc
      121 gcagcgaccg ccgcgtcgag cgcgcagaac gcggcgaacc cgaccgcacg tttgggaatg
      181 cttaacttgt cgccggtcgg aaacggatga acgcgggtga tgctgcccca gttggtcttc
      241 tcgcggcgca cgatgcggcc gcgccatgcg ggcagcaccg cgtggtgctc gtaccacggc
      301 aacgacgtca cgacatcgac atgatggcca agaccgccga gctcttcgac gaggcggctc
      361 gcgatcacgc cggtcggcgc gacgtcgggc tcgaaatgcg ggagcacggc gaggatcttc
      421 acgaggcggc tctttggtag gcgtcgacga ctagcgcaac cggatcaacc gcggccaact
      481 cggttgcgcg ggcacgaccc gcgtcggcga ggcgttgcct tgcgtcgggg ttgtccaaca
      541 aaccgttcat cgcgtcggcc cacgcaccga cgtcgtcggg gtcgacgagc aggccggcgt
      601 cgccgacgag tgcgggcaat gccgtcgcgt cggccacgat caccgggcag ccgacgctca
      661 tcgcttcgac gaccgggatg ccaaaaccct cgaaacggga gggaaaagcg agcatcgaag
      721 cttcttcata caggacgccg agctgctcgg cggtcacgta gccgggcatc cgcacgtggc
      781 gatcgagacc aagccgtgcg acgaggtcgg agatctctgc gcgtgtcgag tgcgccgaac
      841 cccaggcgcc ggcgccggcc gcacccgtca gcacgagtga gacgtcgtgg tgcttcacga
      901 cctcggcaaa cgcgttcacc aacagcgcat gattcttgtg cgggtaggcg gcggccggat
      961 tgaggatgaa atcgccgcgc acaccgagtt catgtagcac cgcggttcgg cgctgtgttg
     1021 acaccgctga cagcggtcgg atcacaggcg gtacgacgat caccttgtcg gcgtccagat
     1081 cgaaagcgtc gatggcctgc gtgcgggtga attcgctcac cgccatcacg actcgagccc
     1141 gcttcagcga actgccttgc gtggcggaaa gatagcgacg cttggccgcg ctgaagtagt
     1201 cgggatacgc caggtactgc aagtcgtgaa gcgtcacgac ggaaggcacc cggcaacccg
     1261 gcggcacgat gccgccgagg tgatgcacaa gtccgatgtg ctgggcacgc aaatgccttg
     1321 gcagccacgc attttcgacc agcacccgcc tgacgacgtg gcgcccggta ggcatcggcg
     1381 gcacgacggt atgtgcggcc gttgctaacg cagggtaggt cgtcacgaaa tcgggcagtg
     1441 cataggcgac gacgtcgacg tcgtcacgtt cgacgagccg ctcgagcagg cccgtcgcgt
     1501 agctctcggt gccgccgacg actcccggca cgagccacag caggttgacg ccgacggtga
     1561 gcgtcacgac gccacctggc ggtacgcctc agcggtgagc tcggcggctt tgttccaggt
     1621 gtacacggcc gctttctccc gcccggcctt ggcgagctcg tcgtgtcgat cacccatcgc
     1681 tttttccaag ccccgagcca tggcgtcggc atcgagcgga tcgaccagga agccggcgtc
     1741 gccgacgaac tccgccatcg cggtaccgct cgacgtgacg accggaactc cgtgtgccat
     1801 cgcctcgagc acgggcagcc cgaacccttc gcgcaggctc gggtagcaga acgcccgtgc
     1861 gccggcgtag gcggcatgca ggtcttcggc aggcaggaat cccaacaagt ggacgcctgc
     1921 cgcctcagtc ggcacgtcgc cccaaccagt cgggccgacg agcaccagtg cgagcgacgg
     1981 atcgctacgt cggacttggg cgaacgcccg gagcagggtc ggcacgttct tgcgcggctc
     2041 tagcgtgccg caccagagca cgtagcgttc gggcaagtga tgttggctca cgaacgcagc
     2101 gatctgcgac gcgctgacat cggggacggc gaccccgtgc ggcacgagtc gcaatcggga
     2161 cgcatcgaaa ccggcaccga cgcagtcgtc gagggtcgac tgcgatggca cgagaaccag
     2221 atcggcctgg gtacgcgcaa gggcgagtcc gcgcgtgaaa aagcggcgtc cgtgcctggt
     2281 gaagtgcgaa ggctcgtgca gaaacgcgag gtcgtgcacg gtcaccacaa gcggtgcgct
     2341 cttcggcggg atcgcgaacg tcgtggcgtg aatgacgtcg atcggaccgg ttgccgcctg
     2401 cacccgcggc caccgcagtg cgtgccacga ttcgtaaagc gcgacccgtg gcagcgacag
     2461 ctggtagata ttgacggtcg gcgtccacgg cgccggcggc aacgtgtcgt ggcgcgctgc
     2521 taccccgaga acctcgatgt cggaacgttt ctggagggcc ttcaccgtct cgagcgctga
     2581 cgacgcggta ccccctggca cgtcgtgcca gcactgctcg agcgtgacgg ccgcgcgcaa
     2641 tctcaccggt cgacgcgtac ggttcgcccg gcgagttgat ccgcgagcat cgcaatgtcg
     2701 gcgtcgacca tcagtcgcgc gagttcggga gtgtgcgtcg tcgcctgcca tcccaaagcg
     2761 gccttggcct tcgacgcgtc gccgcacaag tcgtcgacct cggcggggcg gaggtaggcg
     2821 ggatctgtct cgacgtactt gcgccagtcg agcccagcgc gctcgaaagc gaactcgacg
     2881 aactgctgga tcgaatacga cgcaccggtt gcgatgacga agtcgtcggg ctcgtcgacc
     2941 tggagcatct gccacatggc ctcgacatat tcaggtgcat aaccccagtc gcgcttggcg
     3001 tcgagattgc ccaggtaaag cttgtcttgt aatccagctt gaatacgcgc gatggcgcgg
     3061 gtgattttgc gcgtcacgaa ggtctcgccg cgccgctcac tttcgtggtt gaacaggatt
     3121 ccgttggtcg cgaacattcc gtacgactcg cggtaattta ccgtgaccca atacgcgtac
     3181 atcttggcag cgccatacgg cgaacgggga tggaatggcg tgttctcgtt ttgtggcggg
     3241 ggagtcgaac cgaacatctc agagctcgac gcctgataga accgcgtcga aattcccgac
     3301 gaacgaatcg cttcgagcaa acggatcgtg cccatgcccg tgacgtcgcc cgtgtagtcg
     3361 ggcatctcga aactgacctt cacgtggctc tgtgcaccga ggtggtaaac ctcgtcgggc
     3421 tcgacggcgt gcatgagatt gaccaggcca acgccgtcgt gcaggtcgcc gtagtgcagc
     3481 cggagccgca cgtcgctgtc gtgcggatct tggtagatgg ggtcgagccg ctcggtgttg
     3541 aaggtcgacg cccgacggat caaaccgtgg acctcatacc ccttcgagag cagcagttcg
     3601 gccagatacg agccgtcttg gccggtgata ccggtcacca gcgcggtctt gccggaagga
     3661 gagacagacg tggaaacggt ggcctccgca tcgcttcgcg ggatggtcgt ggcggccatc
     3721 gcgcaa
//
LOCUS       DASWVF010000583         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_41715, whole genome shotgun sequence.
ACCESSION   DASWVF010000583 DASWVF010000000
VERSION     DASWVF010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41715"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGD55_10235"
     CDS             complement(<1..243)
                     /locus_tag="VGD55_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0870764.1"
                     /translation="MPAFIVRGPLDVSTAATLRAQLTGLLATDGTDDLVVDLHRMGPL
                     DSVGLGLLVAIHRQAQRGGRRLVLIGVPPRVMRVLTA"
     gene            complement(374..1231)
                     /locus_tag="VGD55_10240"
     CDS             complement(374..1231)
                     /locus_tag="VGD55_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0870765.1"
                     /translation="MTRVAVVGAGLMGAGIAQVAARAGYDVVLHDTTPKSLDRATRAI
                     AASQARLVARGELTEADAEASRARIVTTTDLDNIASAEIVVEAVFEQLEVKLEVFREI
                     DARAAPGAVLATNTTAIPITKIAAATSRPQDVVGTHFFSPVPVMQLCELVRGDATSNE
                     TLARARAFAESIGKTCVVVERDVAGFVTSRLLVAFVNEAIALVEAGIATPADIDVACR
                     LGFGHTMGPLATVDLTGLDVILHAARNIYTETGEEKFAPPGLLEQMVADGLLGRKSGQ
                     GFYSYERQD"
     gene            complement(1228..2508)
                     /gene="murA"
                     /locus_tag="VGD55_10245"
     CDS             complement(1228..2508)
                     /gene="murA"
                     /locus_tag="VGD55_10245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0870766.1"
                     /translation="MERIRVAGSTRLAGEVFISGAKNSVLKLMAAALLAPGRTTITAV
                     PGILDVDIMAELLRRLGCDVDSTPGRLILDVPDRIGHEADYDLVRRLRASIAVLGPLL
                     ARCGEARVALPGGDNIGSRGLDLHVAGLQKLGAELDSEHGFLIARAPRGLVGAPVWLD
                     FPSVGATENLMMAGVLARGTTIIDNAAREPEIVDLARMLQGMGAKVDGAGSSTIEIQG
                     VTALSPATHATVPDRIVAGTFAMAAVMTQGDIEIHNARPEHLEIVLDRLVGSGAAVSE
                     LDDGFHVEMNGRASAVDVVTLPYPGFPTDFLPMIIGLNSVAEGAAMVTENVFEGRFRF
                     IQELVRLGADIRTDGHHAVIRGRERLSGAPVEATDIRAGAGLVLAGLVADGVTTVSEV
                     HHIDRGYAGFVEQLQALGADVVRESDPGASPLMS"
     gene            2551..3138
                     /locus_tag="VGD55_10250"
     CDS             2551..3138
                     /locus_tag="VGD55_10250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0870767.1"
                     /translation="MAVHLTRIYTKTGDTGTTALVDGSRTSKADARLMAFGDCDEANS
                     ALGVALALGGLPLDVRELLVRVQNDLFDVGADLATPVVANPRTLPLRILEADIERIES
                     AIDHYNAQLPDLASFVLPGGSAGAALLHVVRTIVRRSERHTWAALAEFGASMSPLPAR
                     YLNRLSDLLFVLARYANTTAAGGAGDVLWKPAGSR"
     gene            complement(3146..4231)
                     /locus_tag="VGD55_10255"
     CDS             complement(3146..4231)
                     /locus_tag="VGD55_10255"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0870768.1"
                     /translation="MTVDAFRDLERALLKVAFGPDDIAHAAWARTGPALDIDQLPFEL
                     HGLFPLIAQALRRLDLQPAELPRFDGVRKRLWTLNSVRLRVLEDACGLLSASGVQPVV
                     TGGMAVLLYRDDIGLRPLTEVDVGLPLEHGEQATRCLENAGWIVKRRRRDGFLMEQRA
                     IVMEKAGQTLTLRWAEPSLTSGRSYGGRDGGSPVLRLHPAVGAGLLLESPADVLTFTL
                     VDGLSLPGYLPVRRSTDALLLGSLLDPAVDWGAFLGRVARKQAQPEVAGQVRRLADLC
                     GAPPQWVANELATAKVPLRERLVLGRDRSPAVTASLRRGRRRNSVAALVGVRRELADI
                     WDLADAHAVGIAAAQRLARRRRGRSEI"
     gene            complement(4269..>4791)
                     /locus_tag="VGD55_10260"
     CDS             complement(4269..>4791)
                     /locus_tag="VGD55_10260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY0870769.1"
                     /translation="VRMMRQDRGGIGAARNVAVSMATGEFLTFLDADDRFPADKVDVQ
                     LRAFDADPSLDVVYGHVREFVSPDVTAEQAARIRPAVDYHVSHLSGVMLIRRSAFDKV
                     GPWKVGLKVGTGVEWYTRSCEQNLRTAILPEVLLERRLHLSNNGLQQADHRLQYAAIV
                     KAALDRRRLQGSG"
BASE COUNT          877 a         1635 c         1500 g          779 t
ORIGIN      
        1 tgcggtcagc actcgcatca cacgaggcgg gacgccgatc agcaccaacc gccgtccgcc
       61 tcgctgtgct tggcggtgaa tagcgacaag cagcccaaga cccaccgaat ccagtggtcc
      121 catgcggtgc aggtcgacga cgaggtcgtc ggtgccgtca gtagcaagca ggcccgtcaa
      181 ctgggcacgc aacgtggctg ctgtcgagac gtcgagcggc ccacgcacga taaaagctgg
      241 catccgccct cctcattaat tgcccttcgg gccaccccgt cgcaccctta gacgcaccat
      301 ctgggcgtcc ggttgcctcc gcagcttcac aaatcagaca agattcaccc agccgacacg
      361 ccggaccgtt cggtcagtct tggcgttcgt agctgtagaa cccctggccg gacttgcggc
      421 cgagcagccc gtcagcgacc atctgctcga gcagtcccgg cggagcgaac ttctcctcgc
      481 cggtctcggt gtagatgttg cgcgcggcat gcaggatcac atcgagcccc gtcaggtcga
      541 cggtcgcgag cggacccatg gtgtgcccga agccgagcct gcatgcaacg tcgatgtcgg
      601 cgggcgtcgc gatgccggct tcgacgaggg cgatcgcctc gttcacgaac gcgacgagca
      661 gccgcgatgt aacgaagccc gcgacgtcac gttcgaccac gacacaggtc ttgccgatcg
      721 actcggcgaa ggccctcgca cgggcgagtg tctcgttcga ggtcgcgtcg ccacgaacga
      781 gttcacacag ttgcatcacc ggaaccggtg agaagaagtg agtgccgacg acgtcctgtg
      841 gtcgcgatgt cgcagctgcg atcttggtga tcgggatcgc ggtcgtattc gtcgcgagca
      901 ccgcgccggg tgcggcgcgc gcgtcgatct cgcgaaagac ctcgagcttg acctcgagtt
      961 gctcgaagac cgcctcgacg acgatctccg cactcgcgat gttgtcgagg tcagtcgtgg
     1021 tcacgatcct tgcgcgtgag gcctcggcgt ccgcctcggt gagttcccca cgagcgacga
     1081 gccgcgcctg cgacgcggcg atcgccctcg tcgcacgatc gagcgacttc ggcgtcgtgt
     1141 cgtgcagcac cacgtcgtag ccggcccgag ctgctacttg tgcgatgccg gcacccatca
     1201 atccggcacc caccactgcg acgcgcgtca cgacatcagg ggcgaggccc caggatcgga
     1261 ttcgcggacc acgtcggcac ccaacgcttg cagttgctcg acgaaccccg catatccgcg
     1321 gtcgatgtga tgcacctcgc tcacggtagt cacgccgtcg gcaacaagac cggcgagaac
     1381 aaggccggcc ccagccctga tgtcggtcgc ctcgacgggc gcacccgaga gcctttcgcg
     1441 accgcggatc actgcatgat gaccatcggt cctgatgtcg gcaccgagcc tgaccaactc
     1501 ttgaatgaag cggaagcggc cttcgaacac gttctcggtc accatcgcgg cgccctcggc
     1561 gaccgaattc aagccgatga tcatcggaag gaagtcggtc ggaaatccgg gatacggcaa
     1621 cgtcacgacg tcgaccgccg acgctcgacc gttcatctcg acatggaagc cgtcgtcgag
     1681 ctcgctgact gcagcgccag aacccacgag acggtcgagc acgatctcga gatgctcggg
     1741 ccgcgcgttg tgaatctcga tgtcgccttg cgtcatgacc gcggccatcg cgaacgtgcc
     1801 ggcgacgatg cggtctggca ccgtcgcatg agttgcgggt gacagtgccg taacgccctg
     1861 gatctcgatc gtcgaactac cggcgccgtc gaccttcgca cccatgcctt gcagcatccg
     1921 tgccaggtcg acgatctcgg gttcgcgcgc ggcgttgtcg atgatcgtgg taccgcgtgc
     1981 aagcacaccc gccatcatca ggttctcggt tgcaccgacg ctggggaagt cgagccacac
     2041 cggcgcaccg acaagaccgc gaggagcccg cgcgatcaag aacccgtgct cgctatcgag
     2101 ttcggccccg agcttttgca atccggccac gtgcaggtcg aggccgcgcg agccgatgtt
     2161 gtccccgccg ggcaacgcga cccgagcctc gccgcaccga gcgagcaacg ggccgagcac
     2221 cgctatcgat gcgcgcagtc gtcggaccag gtcgtagtcg gcctcatgac cgatccgatc
     2281 ggggacgtcg aggatcagcc gccctggcgt gctgtcgaca tcgcacccca ggcggcgcaa
     2341 gagctcggcc atgatgtcga cgtcgaggat gccggggacc gcggtgatcg tcgtccggcc
     2401 cggcgcaaga agagccgccg ccatcagctt gagcacactg ttctttgcac cgctgatgaa
     2461 cacctcaccc gccagcctgg tgctgcccgc gaccctgatc cgctccatcg cggcatggta
     2521 gtcccgggcg ggcgcattag gctcgagacc atggctgtgc acctgacccg catctacacg
     2581 aaaaccgggg acaccggaac gaccgcgctc gtcgacggct cccgcacctc caaggccgac
     2641 gcccggctca tggcattcgg cgactgcgac gaggcgaact cggctctcgg cgtcgcgctg
     2701 gcgctcggcg ggctgcccct cgacgtccgc gaactgttgg tgcgcgttca aaacgacctg
     2761 ttcgacgtcg gggccgacct cgcgacgccg gtcgtcgcga acccacgcac gctgccgtta
     2821 cggatcctgg aagccgacat cgagcgcatc gagagcgcca tcgatcacta caacgcacaa
     2881 ctgcctgatc tcgcgagctt cgtactaccc ggaggaagtg cgggtgcggc gttgctccac
     2941 gtcgttcgca cgatcgtgcg tcggtctgaa aggcacacgt gggcggcact ggcggagttc
     3001 ggtgcgtcga tgagcccatt acctgcgcgc tatctgaacc ggttgtccga cctgctcttc
     3061 gtgctcgcgc gttatgcgaa cacaacagcg gccggcggtg ccggcgacgt gctctggaag
     3121 cccgcaggca gccgctgatc gcgccttaga tttcggagcg acctcggcgc ctgcgtgcga
     3181 gtctctgggc cgccgcgatg cccacggcgt gcgcgtcggc gagatcccag atatctgcga
     3241 gttcgcgacg gacgccgacc aacgcggcga cggaattgcg gcggcgaccc ctgcgcaggg
     3301 atgcggtcac ggcgggagag cgatctcggc ccagtacgag ccgttcccgc aaaggtactt
     3361 tcgcggtcgc gagttcattc gcgacccact ggggaggtgc accgcagaga tcggctagcc
     3421 gcctcacctg cccggcgacc tcgggttgcg cctgcttgcg cgcaacccgc ccaagaaaag
     3481 cgccccagtc gacagcggga tcgagcagcg aaccgaggag caacgcatcc gtactgcgcc
     3541 gaaccggaag atacccaggc aggctcaacc cgtcgacaag cgtgaaggtg aggacgtcgg
     3601 ctgggctctc gagcagcagc cccgctccaa ctgctggatg aagccgtagg accggtgaac
     3661 caccgtcgcg gccgccgtag gagcggccac ttgtcaacga aggctcggcc caacgcaagg
     3721 tcagagtctg acctgccttt tccatgacga tcgcccgctg ctccatcagg aagccgtcgc
     3781 ggcgccgcct cttgacgatc catccggcat tctcgagaca acgcgtcgct tgctcgccgt
     3841 gttcgagcgg cagcccaacg tcgacctccg tgagtggacg taggccgatg tcatcgcggt
     3901 acaagagcac cgccattccg cccgtaacca ccggttgcac gccactcgct gagagcagac
     3961 cgcaggcgtc ctcgagaaca cgcaggcgga ctgaattcaa ggtccagaga cgtttccgaa
     4021 caccgtcgaa ccgcggaagc tcagctggtt ggaggtcaag tcgacgcaac gcttgtgcga
     4081 tgagcgggaa caggccgtgc agctcgaacg gcagctgatc aatatcgagc gccggaccgg
     4141 tacgtgccca cgccgcatgc gcgatgtcat ccgggccaaa tgccaccttg agcaacgcgc
     4201 gttcaaggtc gcggaaggca tccacggtca tgcgccaata accgtcatgc gctaaccacc
     4261 gtcatgagct agccgctgcc ctgcaggcgg cgccggtcga gcgcggcctt gacaatagcg
     4321 gcgtattgca atcggtggtc cgcctgctgc agtccgttgt tcgataggtg cagtcgccgc
     4381 tcgaggagaa cctcgggcag gatcgccgtc ctgaggttct gttcgcagga tcgcgtgtac
     4441 cactcgactc ctgtgccgac cttgagcccc actttccacg ggccgacttt gtcgaacgcc
     4501 gagcgtcgga tgagcatcac cccggacaaa tgcgaaacgt ggtagtcgac cgcaggtcgg
     4561 atccgagcgg cctgctctgc ggtgacgtca ggactcacga actcccgcac gtggccatac
     4621 acaacgtcga gcgacgggtc ggcgtcgaat gcgcgaagct gcacgtccac cttgtccgcc
     4681 gggaagcgat catcagcatc gagaaaggtc aagaattcac cggtcgccat cgagaccgcg
     4741 acattgcgcg cggctccgat accgccgcga tcctgcctca tcatccgcac c
//
LOCUS       DASWVF010000584         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_143857, whole genome shotgun sequence.
ACCESSION   DASWVF010000584 DASWVF010000000
VERSION     DASWVF010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143857"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGD55_10265"
     CDS             complement(<1..274)
                     /locus_tag="VGD55_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870770.1"
                     /translation="MTDFAQLETTARRVRGHAIRMATNGGCFLGASLSCADILVHLYG
                     RVLRTAPDRAQDPTRDVLLLSKGHDVPALYGTLAEAGYFPVSRLANH"
     gene            complement(271..1119)
                     /locus_tag="VGD55_10270"
     CDS             complement(271..1119)
                     /locus_tag="VGD55_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEY0870771.1"
                     /translation="MLAAGVQTKAGRPNVVAVIQARTGSTRLPGKVLRPVAGRPLLEW
                     MLDRLAWATELDDLVVATTWLAADAPIRALCDRRGVSWFGGHPTDLLDRHLKVGRARG
                     ADAVVKIPSDCPLIDPSIVDLVVARFRVEFADVAFVSNLHPPTWPDGNDVEVVRMDAL
                     EEAWCLATRAFEREHTTPFIWDQPDRFAIANVAWPRGEDLSATHRLTLDYEEDLQLIG
                     EVLRALHRPDRPFTVDEIVDLLDARPELRALNARHLGSSWMSRHAADLKTLAGAPVAT
                     EKEARP"
     gene            complement(1229..>1587)
                     /locus_tag="VGD55_10275"
     CDS             complement(1229..>1587)
                     /locus_tag="VGD55_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0870772.1"
                     /translation="KDQCSFKTDSDQLMPGCDAKLKKLANTLIDVKKHLNVSGVTSFK
                     FEVSGHTDTSGKPEHNKELSAKRAATIAHELESRGLASNEIISVGMGADRPLVTPDDT
                     PAKKAKNRRYEIQIRF"
BASE COUNT          243 a          520 c          543 g          281 t
ORIGIN      
        1 ggtggttcgc cagacgcgac accgggaagt accccgcctc ggccagcgtg ccgtaaagag
       61 ccggtacgtc gtgccccttc gacagcaaga gaacgtcccg ggtcgggtcc tgagctcggt
      121 caggcgccgt ccggagcacc cgcccgtaga ggtgcaccag gatgtcggcg cacgacagcg
      181 acgcgcccag gaagcatccc ccgttcgtcg ccatccggat cgcatgtccg cgcacgcggc
      241 gcgcggtcgt ctcgagctgc gcgaagtcgg tcacgggcgc gcttccttct cggtcgccac
      301 gggtgcgccg gccagggttt tcaggtcggc agcgtgccgg gacatccagc tactgcccag
      361 gtggcgtgcg ttcagcgccc gcagctccgg gcgcgcgtcc agcaggtcca cgatctcgtc
      421 gaccgtgaac ggccggtcgg gacggtgaag ggcccgcaag acttcgccga tgagctgcag
      481 gtcttcttcg tagtccaggg tcagccggtg cgtggcggag aggtcctcac cccgcggcca
      541 cgccacgttg gcgatcgcga accgatctgg ctgatcccag atgaacgggg tcgtgtgttc
      601 gcgctcgaaa gcgcgcgtgg cgaggcacca ggcttcttcg agcgcgtcca tccggacgac
      661 ctcgacatcg ttgccgtcag gccaggtcgg cgggtgcagg ttgctgacga acgcgacgtc
      721 ggcgaactcg acccggaatc gcgccacgac gaggtcgacg atcgaggggt cgatgagagg
      781 gcagtcggac ggaatcttga cgaccgcgtc ggcgccgcgc gcgcgaccga ccttcaggtg
      841 gcgatcgagc agatcggtcg ggtgcccacc gaaccaggag acgcctcttc ggtcgcacag
      901 ggcccggatc ggcgcatccg cggcgagcca ggtggtggcg accaccaggt cgtcgagctc
      961 ggtcgcccag gccagacgat cgagcatcca ttccagcagc ggccgcccgg ccactggacg
     1021 gagcaccttt ccgggcagcc gggtcgaccc cgtacgggcc tggatgacgg cgacgacgtt
     1081 cggtcgccca gctttggttt gcacgccggc agcgagcatc tgatcatgct tgttaccggc
     1141 ttttcaccgc gctctcggaa actgatcggt tccgaattgt cgacagccgt caccaagatg
     1201 acggcatcgc gacagtcgcg tggccgtctc agaaccggat ctggatctcg tagcgccggt
     1261 tcttggcctt cttggcgggc gtgtcgtcgg gggtgaccag cgggcggtcc gcgcccatcc
     1321 ccacggagat gatctcgttc gaggcgagcc cgcgcgactc cagctcgtgc gcgatcgtgg
     1381 ccgcgcgctt ggcggacagc tccttattgt gctcgggttt gccgctggtg tccgtgtggc
     1441 cagaaacttc gaacttgaag ctggttacgc ccgagacgtt caagtgtttc tttacgtcta
     1501 tgagggtgtt cgccagcttc ttgagcttgg cgtcacagcc cggcatgagc tgatcgctgt
     1561 ccgtcttgaa gctgcactgg tccttgc
//
LOCUS       DASWVF010000585         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_118303, whole genome shotgun sequence.
ACCESSION   DASWVF010000585 DASWVF010000000
VERSION     DASWVF010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118303"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..631
                     /locus_tag="VGD55_10280"
     CDS             155..631
                     /locus_tag="VGD55_10280"
                     /inference="COORDINATES: protein motif:HMM:NF022199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HEY0870773.1"
                     /translation="MRVVLDFVDGAGWIVVFAVVLLIAFVVRRGAVRRRGATLHCGLR
                     MGVTATNPSSRGWHTGMARYTPEDLEWFRLLSLRYQPNLTFVRRSIEITSRRDPRGAE
                     TFAVPRDAVIVTCNVITRAGDPLSVEFAMSDAAVTGFLAWIESSAPGAHSHPDTRV"
     gene            843..>1811
                     /locus_tag="VGD55_10285"
     CDS             843..>1811
                     /locus_tag="VGD55_10285"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0870774.1"
                     /translation="MTVSSRVRPSTKTRAAQPLARLLLDSIDDSVRGTVSSAAYAGIE
                     PDALESAVFIHKIAPAVYVHLRDSGDAPASLIEPMMQRYQLQVARQLQVLSDLAGLRN
                     VLTDTGVAWAAMKGPVLAERLWSRPDLRTYIDLDILVDRRRFGDVLDTLIAHGATMVD
                     QNWSLIEQQVRAEVSLTLPNGTALDLHWHVVNQRRLRSQFTFPIADMLARAVDAKIGG
                     TTVPTLDPSDTLLHLAYHTAHSGGHRLMWLKDVERAAADRDLDWAETLRRARAYHVRL
                     PLAIVLARTAKAIGFEVPPPAAAFAPAKHSAWGLFAATTDRWSPVPV"
BASE COUNT          273 a          587 c          601 g          350 t
ORIGIN      
        1 actcgcccga gcaacggcag ggtcggacgc cgggtcggcc ggcgtgggtg acggtggtga
       61 cggctcgaac gaggcggctg ccgcacgcgc ccgagcgcga ctcgcggccg ccggtcagcc
      121 cgtctgacgc acgctaagac ggggagcgga cgagatgcga gttgtccttg acttcgtcga
      181 tggcgccggc tggatcgtcg tctttgctgt tgtccttttg atcgcgtttg tggtgcgtcg
      241 cggtgccgtg cgacgccgtg gtgcgacctt gcattgcggg ctgcgcatgg gcgtcacggc
      301 cacaaaccca tcgagtcggg gttggcacac cgggatggct cgctacacac ccgaagacct
      361 cgagtggttc cggctgctca gcttgcggta tcagccgaac ttgacgtttg ttcgccgcag
      421 catcgagatc acgagtcgcc gtgatccgcg cggcgccgag acattcgccg tcccgcgtga
      481 tgctgtgatc gtcacctgca acgtgatcac ccgtgcgggc gacccattga gcgtcgaatt
      541 tgccatgagc gacgctgccg tcactgggtt cttggcgtgg atcgagtcgt cagctccggg
      601 cgcgcattcg caccccgaca cgcgggtcta gctgggtcgt ccctggtatt gctccgttcg
      661 gagtagtccg cgaccatccg gcctgtcagt ttgggtggcc gaaggtacca atccggggcc
      721 tcaggggcgg ctttgtggtg tgtatcaagg ccatgttcgt tcactcaccg ttagggtagc
      781 cacgccgcat gacggcgaag ttgtagcggt gttgaggtga agaaccaggt ggggtgcgtg
      841 ctttgaccgt cagttcccgc gttagaccgt cgaccaagac cagagcggct cagccactgg
      901 ctcggctgct actcgactcg atcgacgact ccgtgcgcgg cactgtttcg tcggccgcgt
      961 atgccggtat cgaacccgac gcccttgagt cagcggtctt cattcacaag atcgcgccgg
     1021 ctgtctacgt gcatctgcgt gactcgggcg acgcgcctgc gagcctcatc gagccaatga
     1081 tgcagcgcta ccagttgcag gtggcccgcc aactacaggt gttgtcggac ctcgccggcc
     1141 ttcgcaatgt gctgaccgac acgggcgttg cgtgggcggc gatgaaaggc ccggtgctcg
     1201 ccgaacgcct gtggtcacgg cccgatctgc gtacctacat cgacctcgac atcctggtcg
     1261 atcggcgcag gttcggcgac gtgctcgaca ccctgatcgc ccacggcgcg acgatggtcg
     1321 accagaactg gtccctgatc gagcagcagg ttcgcgctga ggtctcgttg acgttgccca
     1381 acggcaccgc tctcgacttg cactggcacg tcgtgaacca acgccgattg aggtcgcagt
     1441 tcacgttccc gatcgccgac atgctcgctc gcgcggtcga cgccaagatc ggtgggacga
     1501 cggttccgac cctcgacccc tccgacacct tgcttcatct ggcgtatcac accgcgcact
     1561 ccggcggtca ccggttgatg tggctgaaag acgtcgagcg tgccgccgct gaccgtgacc
     1621 tcgattgggc ggagacgttg aggcgggccc gggcctacca cgtgcgactg ccgctggcca
     1681 tcgtgctcgc gcggacggca aaggcgatcg gcttcgaggt accgccgccc gcagcggcct
     1741 tcgcgccggc caagcactcg gcgtggggtc tgttcgcggc gacaaccgac cgctggtcgc
     1801 cggtccctgt c
//
LOCUS       DASWVF010000586         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_500648, whole genome shotgun sequence.
ACCESSION   DASWVF010000586 DASWVF010000000
VERSION     DASWVF010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500648"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="purN"
                     /locus_tag="VGD55_10290"
     CDS             <1..489
                     /gene="purN"
                     /locus_tag="VGD55_10290"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0870775.1"
                     /translation="QHTQAIERAQRTGVPTFVLELSQFTDRAEWDAALTEAVAVYAPD
                     LVILAGFMKLVGPVFMAKFDGRIVNTHPALSPSFPGMHAPRDALAYGVKVTGCTLFVV
                     DDGIDTGPIVAQAAVPVLDDDDESTLHERIKNVERQLLVDTVAAMARQGWTVTGRKVT
                     IP"
     gene            486..>1971
                     /gene="purH"
                     /locus_tag="VGD55_10295"
     CDS             486..>1971
                     /gene="purH"
                     /locus_tag="VGD55_10295"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0870776.1"
                     /translation="MSTDRRRIRRALISVYDKTSLAELGQGLASAGVQIVSTGTTAAV
                     LAENGVPVVKVEDLTGFPECLDGRVKTLHPKVHAGLLADTRKPEHLRQLEELGIEAFD
                     LVVCNLYPFRETAARGAEPDAVIEQIDIGGPSMVRAAAKNHPSVAVVVSPARYLEILD
                     ALSDGGFTLEQRKALAVDAFVHTASYDIAVASWLGSTYAPTDDGTGFPAWVGASWHRG
                     EVLRYGENPHQRAALYRADHGSPGLAQARVLHGKAMSYNNYVDTDAARRAAYDFTQPC
                     VAIIKHANPCGIALGADIAEAHRKAHACDPASAYGGVIATNRHVTLELAQQIAEVFTE
                     VVCAPGFDSAALDLLTQRKNLRLLECAGMHPGRTVETRQISGGMLMQSVDDIEADGDD
                     SANWTLQAGAAADPSTLADLDFAWRASRSVKSNAILLATDRATVGVGMGQVNRVDAAR
                     LAVWRAGDRSSGSVAASDAYFPFADGLQLLIDAGVRAVVEPGGSI"
BASE COUNT          326 a          648 c          674 g          323 t
ORIGIN      
        1 cagcacaccc aggcgatcga acgggcgcag cggaccggcg tcccgacatt tgtcctggag
       61 ctttcgcagt tcaccgacag ggccgagtgg gacgccgcgc tcaccgaggc agtcgctgtc
      121 tacgcgcctg acctcgtgat cttggccgga ttcatgaagt tggtcgggcc ggtgttcatg
      181 gcgaaattcg acgggcggat cgtgaatacg catccggcgc tgtcgccgtc atttcctggc
      241 atgcacgcgc cgcgcgacgc cctcgcctac ggcgtcaagg tgacgggctg cacgttgttc
      301 gtcgtcgacg acgggatcga cacgggtcca atcgtcgcgc aagccgcagt gcccgtgctc
      361 gacgacgacg acgagtcaac gctgcacgaa cgcatcaaga acgtcgaacg ccaactgctc
      421 gtcgacactg tcgccgcgat ggcccggcag ggttggaccg tcaccggaag aaaggtcacc
      481 atcccgtgag taccgatcgg cgtcggatca ggagggcgct gatcagcgtc tacgacaaga
      541 caagcctggc cgagcttggt caggggttgg cgagcgccgg cgtgcagatc gtctcgacgg
      601 gcacgaccgc cgcggtgctc gcagagaacg gcgtgccggt cgtcaaggtc gaagacctca
      661 ccggatttcc cgagtgcctc gatggccggg tgaagacgct gcacccgaag gtgcacgccg
      721 gtctgctcgc cgatactcga aagcccgagc accttcgcca gctcgaagaa ctcggcatcg
      781 aggcgttcga cctggtcgtg tgcaacctgt acccgtttcg ggagaccgcc gcgcggggtg
      841 cggagcctga tgcggtgatc gagcagatcg acatcggcgg gccgtcgatg gtgcgggccg
      901 ctgcgaagaa ccacccgtcg gttgccgtcg tcgtatcgcc cgcgcggtat ctggagattc
      961 tcgacgcgct gtctgatggc ggcttcactc tcgagcaacg caaggcgctc gccgtcgacg
     1021 cgttcgtgca caccgcgtcg tacgacatcg cggttgcgtc atggttgggc agcacgtacg
     1081 cgccgaccga cgacggcacc gggttcccgg cctgggtcgg cgcgagctgg caccgaggcg
     1141 aggtactgcg gtacggcgag aacccacacc agcgggctgc gttgtaccgc gcagaccacg
     1201 gctcgcccgg actcgcgcaa gcgcgcgttc tgcatggcaa ggccatgtcg tacaacaact
     1261 acgtcgacac cgatgccgct cggcgtgccg cctatgactt cacgcagccg tgcgtcgcga
     1321 tcatcaagca tgcgaacccg tgcggcatcg cgctcggagc tgacatcgcc gaagcgcatc
     1381 gcaaggcgca cgcctgcgac ccggcgtctg catacggcgg cgtgatcgcc acgaatcggc
     1441 acgtgacgct agagctcgcg cagcagatcg ccgaggtctt caccgaggtc gtgtgcgcgc
     1501 cgggcttcga ttccgctgcg ctcgacctgc tgacgcaacg caagaacctg cgactgctcg
     1561 agtgcgcggg gatgcatcct ggccgcaccg tcgagacccg ccagatcagc ggcggcatgc
     1621 tgatgcaaag cgtcgacgac attgaggcgg acggcgacga ctcggcgaac tggactctcc
     1681 aggcgggcgc cgctgctgac ccctcgaccc ttgccgatct cgacttcgcg tggcgggcat
     1741 cgcggtcggt gaagtcgaac gcgatcctgc tggctactga ccgggccacg gttggcgtcg
     1801 gcatggggca ggtcaaccgg gtcgacgctg ccaggttggc ggtgtggcgg gccggcgatc
     1861 ggtcgtccgg ctcggtggcc gcgtcggatg cctacttccc gttcgccgac gggcttcagt
     1921 tgctgatcga tgccggcgtg cgggcagtcg tcgagccggg cggctcgatc c
//
LOCUS       DASWVF010000587         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_449932, whole genome shotgun sequence.
ACCESSION   DASWVF010000587 DASWVF010000000
VERSION     DASWVF010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449932"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGD55_10300"
     CDS             <1..445
                     /locus_tag="VGD55_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0870777.1"
                     /translation="ERGTARLPVLELTAYGAGAGAVVLAFVSPVWTFPFSALGHSARF
                     AGHATPVWILLSVVVLVGTVLAYLLGMSALAYLPSPSATVIATLEILVAAVVAWGVLG
                     EHMTAAEIAGGIIILTGVVVVAERARLRPELPELEYVEAPDLTRT"
     gene            complement(464..913)
                     /locus_tag="VGD55_10305"
     CDS             complement(464..913)
                     /locus_tag="VGD55_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4439 domain-containing protein"
                     /protein_id="HEY0870778.1"
                     /translation="MSGLRLDAALVAAVAAENAAVYGYGVAGARLATPQEKAAARVAY
                     DAHRAQVDVTTQWVNDLHLGATPPPPAPVYALPTPVTDPASARVALAAIEESTAAAYA
                     DVVAAVLPAGGELQRLAAGWLQAAAVREAQWRTMSVPFPGLVGRLTS"
     gene            complement(910..1563)
                     /locus_tag="VGD55_10310"
     CDS             complement(910..1563)
                     /locus_tag="VGD55_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870779.1"
                     /translation="MPDLPPLTRRTVLRMFAVAAVGLLAGCRSTKRPAAAPAPVSSPA
                     PQATSSSAIATPTLTPQPTLSPDELLAKRVASAEAELVAAYDSAASQHPELASLLAPF
                     RADHAAHADALVPGSASPPPPSPPPSSTPPSSTLPSSTPSSTASPSGSPPSGSPSSIS
                     PPPNPVLTHLADAEHSAAAARIDDLAATTGSLASLLASIGGCEAAHAALLATARPTQ"
     gene            1736..2281
                     /gene="rimP"
                     /locus_tag="VGD55_10315"
     CDS             1736..2281
                     /gene="rimP"
                     /locus_tag="VGD55_10315"
                     /inference="COORDINATES: protein motif:HMM:NF000930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0870780.1"
                     /translation="MLPSSASAGRNLAALLTPAVEDTGLDLEDVAVTRVGHRAEVRVL
                     VDKDGGVSLDDVAQVSQAISTVLDQPEADAILGAEPYVLEVSSPGVDRPLVEPRQWRR
                     AAGRLVRVSLTAGGEITGRILTADAHGVRLAVNPAKPKGAPREQTFTFDELGPGRVQV
                     EFNRPAGTAETDANITDEVAR"
     gene            2278..3354
                     /gene="nusA"
                     /locus_tag="VGD55_10320"
     CDS             2278..3354
                     /gene="nusA"
                     /locus_tag="VGD55_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0870781.1"
                     /translation="MNIDMAALRSLEREKDVSFDLLVEAIESALLIAYQRTEGANAHA
                     RALVDRASGEVTVLATEMDDDGNPIREFDDTPTDFNRIAATTAKQIILQRLRDAEHEL
                     TFGEYAGREGDIVGGVIQQQHDSRNVFVDLGKVEAILPAGEQVPGERYEHGSRIRCYV
                     VQVHKGVRGPQITLSRTHPGLVRKLFALEVPEIADGTVEIAALAREAGHRSKIAVRSN
                     VAGLNAKGACIGPLGQRVRAVMAELHGEKIDIVDWSDDPAEFVGHALSPARVSAVEVV
                     DLANRSARVTVPDYQLSLAIGREGQNARLAARLTGWRIDIRSDNPDANPDANPAAENP
                     APAEAASPAASLQGSERDAQPSGG"
     gene            3383..3652
                     /locus_tag="VGD55_10325"
     CDS             3383..3652
                     /locus_tag="VGD55_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY0870782.1"
                     /translation="MRSCVGCKKRAAKSDLLRLAVVRHDEQFRVVPDPRGQRPGRGAS
                     LHPDPHCLDLAERRRAFPRAFRLAGPLDFTAVRGYIEAQPRGTRA"
     gene            3817..>4096
                     /locus_tag="VGD55_10330"
     CDS             3817..>4096
                     /locus_tag="VGD55_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0870783.1"
                     /translation="MAKVRVYELAKELNVESKAVMAKLQEFGEFVRSASSTIEAPVVR
                     KLRESFPAPAADAAPVPAPRAAATARTADKPRAVEAAPPVVAAPPAPVA"
BASE COUNT          661 a         1335 c         1452 g          648 t
ORIGIN      
        1 cgaacgcggg acggcgcggc tgccggtgct ggagttgacc gcgtacggcg ccggagctgg
       61 cgcagtcgtg cttgcttttg tctcgccggt ctggactttc ccgttctccg cgctcggcca
      121 ctcggctcga ttcgccgggc acgcaacgcc ggtctggata ttgctgagcg tcgttgtgtt
      181 ggtcggcacc gtgctggcgt acctgctggg catgtcagcg ctcgcgtatc tgccatcgcc
      241 gtcggcaact gtcatcgcca cgctcgagat cctggtagct gcagtagttg cttggggtgt
      301 gcttggtgaa cacatgacag cggccgagat cgccggcggg atcatcatct tgactggcgt
      361 cgtggtggtg gccgaacgag cgcgccttcg accggaactt cccgagctcg agtacgtcga
      421 ggcgcctgac ctcaccagga cctaggttcg cccaccgacc gtcttacgac gtgaggcggc
      481 cgacgagccc cgggaacgga accgacatcg tccgccattg ggcttctctg actgcggctg
      541 cttgcaacca gcccgcggcg agacgctgta gctcgccacc ggcgggcagc actgcagcga
      601 cgacatcggc gtacgcggct gcggtcgact cctcgatggc ggcgagggcg acgcgggccg
      661 atgcaggatc ggttacgggc gtcggcagcg cgtacaccgg tgcaggtggc ggcggtgtcg
      721 caccgaggtg caggtcattg acccactgcg tcgtgacgtc aacctgtgct cgatgggcgt
      781 cgtatgccac gcgcgctgcg gccttctctt gcggggtcgc gagccgtgcg cccgcgaccc
      841 cgtagccgta gacggccgcg ttctccgcgg cgacagcagc caccagtgcg gcatcgaggc
      901 ggaggccgct cattgcgtcg gcctcgctgt cgctaggagt gcggcatgag ctgcttcgca
      961 accgccgatc gaggcgagca ggctggcaag cgatccggtg gtcgcggcga gatcgtcgat
     1021 gcgggctgcg gcagcgctgt gttcggcgtc tgcgaggtgg gtcagaaccg ggttgggcgg
     1081 cggggagata gacgacggtg agccggacgg cggtgaaccg gacggcgacg cagtcgacga
     1141 tggcgtgctc gaaggcagcg tgctcgaagg cggcgtgctc gaaggcggtg ggctcggggg
     1201 agggggactc gccgagccgg gcacgagcgc gtccgcatgt gcggcgtggt cggcccggaa
     1261 cggggccaag aggctggcca gttccgggtg ctgcgaagcc gcgctgtcgt aagcggccac
     1321 caactctgcc tcagcgctcg ccacccgttt ggccaataac tcgtcgggtg acagcgttgg
     1381 ttgtggggtg agcgtcggtg tcgcaatagc cgatgagctc gtcgcctgag gcgcgggtga
     1441 gctcaccgga gctggcgcgg cggcgggtcg cttcgtcgat cggcaaccgg ccagcagccc
     1501 cacggccgca acggcgaaca ttcgcagcac ggtccgcctg gtgagtgggg gcaggtccgg
     1561 cacgaggtcc acctggcctc actcctcgcg gacgggaaag gtctgcaacg cggcgacagc
     1621 ttacggaggc gtcggacagc tacgctgggc ccgagcacgt ttgaaccggt cacccgaacc
     1681 ggcgagctca caacgaacca ctgacaacag cagagcaagc gaggaggtcg gcaggatgtt
     1741 gccgtcgagc gcctccgctg gccgcaacct ggccgcgctg ctcacccctg cggtcgaaga
     1801 caccggcctc gacctcgaag acgttgccgt caccagggtc ggccaccgag ccgaagtcag
     1861 ggtgctcgtc gataaggacg gtggggtcag cctcgacgac gtcgcccagg tgagccaggc
     1921 gatctcgacc gtgctcgacc agcccgaagc cgacgcgatc ctcggagctg agccctacgt
     1981 gctcgaggtg agttcaccgg gcgtcgaccg accgctcgtc gagccgcgcc aatggcgacg
     2041 cgcggcgggt cggctcgtgc gggtgagctt gaccgccggc ggggagatca ccggccggat
     2101 cctgacggcc gatgcccacg gcgtccgcct ggcggttaat ccggcgaagc ccaaaggcgc
     2161 gccccgcgag cagaccttca ccttcgacga gctcggtccc gggcgcgtgc aggtcgaatt
     2221 caaccgcccg gccggcaccg cggagaccga tgccaacatc accgatgagg tggcgcggtg
     2281 aacatcgaca tggctgcctt gcgcagcctc gaacgcgaga aggacgtctc gttcgatctg
     2341 ctcgtcgagg cgatcgaatc ggccttgctg atcgcctatc agcgcaccga gggcgccaac
     2401 gcgcatgccc gtgccttggt tgatcgcgcc agcggcgagg tgacggttct cgccaccgag
     2461 atggacgacg acggcaaccc gattcgcgaa ttcgacgaca cgcccaccga tttcaacaga
     2521 atcgcggcca ccacggcgaa gcagatcatc cttcagcgcc tgcgcgacgc cgaacacgag
     2581 ctcacgttcg gcgagtacgc ggggcgcgaa ggcgacatcg tcggcggcgt gatccagcag
     2641 cagcacgact cgcgcaacgt gttcgtcgat ctgggcaagg tcgaggcgat cttgcctgct
     2701 ggtgaacagg ttccggggga gcgctacgaa cacggctcgc ggatccgctg ctacgtcgtg
     2761 caggtgcaca agggcgtgcg cgggccgcag atcaccttgt cgcgcaccca ccccggcctg
     2821 gtccgcaagc tgttcgctct cgaagtaccc gagattgccg acggcactgt cgagatcgcc
     2881 gcgcttgcgc gcgaggcagg tcaccgctcg aagattgcgg tgcgctcgaa cgtcgctggc
     2941 ctgaacgcga agggcgcctg catcgggccg ctcgggcagc gggtgcgcgc tgtcatggcc
     3001 gaactgcacg gcgagaagat cgacatcgtc gactggtcgg acgaccctgc cgagttcgtg
     3061 gggcacgccc tgtctcctgc ccgtgtgagc gcggtcgagg tcgtcgatct ggcgaaccgg
     3121 tcggcgcggg tcacggtgcc cgactaccag ctctcgctcg cgatcggccg cgagggccag
     3181 aacgccaggc tcgccgcgcg cctgacgggc tggcggatcg acatccgctc ggataacccc
     3241 gatgccaacc ccgatgcaaa tcccgctgcc gagaatcccg cacctgccga ggccgccagc
     3301 ccggcggcgt ccctgcaagg cagtgagcgt gatgcacaac cctcaggggg gtagactcgg
     3361 cgtggccgaa cgcgtcgagc ccatgcgctc ttgtgtgggt tgtaagaaac gggcggccaa
     3421 gtccgacctt ctgcgtctcg cggtggtcag gcacgacgag cagttccgcg tcgttcccga
     3481 tccacgcggg cagcgtccgg gccggggcgc ctcgttgcat cccgatccgc actgcctcga
     3541 tctcgccgag cgacgtcggg cgttccccag ggcgtttcgc ctggcgggcc cgctcgattt
     3601 caccgctgtg cgggggtaca tcgaggcgca gccgagaggg acaagagcct agtgttcgag
     3661 ggcctgaagt aaccagagtc gccgtgacgg gaagcaggtc gagcaagtcg atgagcgctc
     3721 gatgagtacg cagcgatgag taaggcgcct gatgaataag taagtgcggc ccgggatcga
     3781 cctgttcgag cacccgggcc aggagacggg agtcacgtgg ccaaggttcg ggtgtacgag
     3841 ctcgccaaag agctcaatgt tgaaagcaag gccgtcatgg ccaagcttca ggaattcggg
     3901 gagttcgttc gatcggcctc ctcgactatc gaggcgccag tcgtccgcaa gctgagggag
     3961 tcgttcccgg cgcctgctgc ggatgcggcg ccggtgccag ctccacgcgc ggctgctact
     4021 gcgcgcacgg ccgacaagcc gcgtgcagtc gaagcggccc cgcccgtggt cgctgctcca
     4081 cctgctcctg ttgcac
//
LOCUS       DASWVF010000588         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_373201, whole genome shotgun sequence.
ACCESSION   DASWVF010000588 DASWVF010000000
VERSION     DASWVF010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373201"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..1094)
                     /locus_tag="VGD55_10335"
     CDS             complement(543..1094)
                     /locus_tag="VGD55_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY0870784.1"
                     /translation="MSGGDPHLSRRQLLVIGVGLGTIGCDSAKQRPPCVGPSDGPGIG
                     CLVGLERLTVAGAASLPVGWATIVGADDNNAAIVAHDALGFYALSATCRHQCCTVTLC
                     DGDCRQPIPSPNDCASPRMAQLATSGAAFLCPCHGSTYGADGSVLNGPSIQPLPALAM
                     QIAGADVIVDLSRSVSSTDRVKA"
     gene            complement(1091..2272)
                     /locus_tag="VGD55_10340"
     CDS             complement(1091..2272)
                     /locus_tag="VGD55_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870785.1"
                     /translation="MTVAVALGPAVGACGGSSSHDAVVPAALRDVERDGEGLVNTTFG
                     DTPTGHLPDWARAQGVLVLLKQVWSNTKAGYPKLPATQVQMVDDAVATLDQAIPGMDQ
                     RAAAFASNKVGLAVPELFDYFHTGAPIGVVRMDAVFRQVGLDGHYGDLTAVQHDLDSL
                     MADWATTKPSVAASVPTCHRVGGTATVVADIDDSLSRLTAAVPSNDMTAIQTVSDEGA
                     LEIDTLELLFDCPKDGPAPASGLGSACSATTACGSGLVCDTAKAGGKCAPDATNKIGT
                     PCTSTNDCGTDSRSACQTEAGDGYRGGYCFMEPCNDVSVCPVGGTCVALGGETPGCFK
                     ACTADTDCRTTEGYVCQLFVTTPPIGFGPTDHACAFPCTRDQDCQQPLKCDVASGKCT
                     P"
BASE COUNT          426 a          824 c          801 g          406 t
ORIGIN      
        1 aatgtaagac gccacaggat ggagaggagc cccttgccgg acggcgtctc gacgaggggg
       61 cgactcgatc gagagttttc tagtagcgca caacgacagc ccggcggacg tgcgggcacg
      121 tgacgaacgc gtcgatcacc gcgccgggca gggagaggcg ccatcgccaa ttcccatgcc
      181 aaccccacac gacccaccgg cgcgggccac ggatgcccgg aatgcttgcg gtgacagtcc
      241 cagaacccaa tgacgattac ctagtcgccc ggcggacaaa agaagcctct gcctttctcc
      301 gctccttctc ggtgtgcgcc ttgacccaat gagtggccca gcatgtcgcg gcgtcgaagc
      361 gctcgtcgtt gtcctcggca aaacagatct cgcagtagaa gggggcatgt cggacacggc
      421 gctgacagac ggagcatctc gaaaaagtcg gcacgacgtg accctttgac acagggatat
      481 catgcgtcca cgaagcgttt ctgccgcgcc caatttgacg cgagccgact ctgagcatcc
      541 gtctacgcct tcacgcgatc cgtcgaactc acagaccgac tcaggtcgac gatcacgtcc
      601 gctccagcga tctgcatcgc gagcgccggg agcggctgaa tcgagggacc gttcaacaca
      661 gagccgtcgg caccgtaggt gcttccatgg caggggcata ggaaagctgc accgctcgtg
      721 gcgagttggg ccatccgagg cgatgcgcaa tcgttcgggc tcggaatggg ctgccgacag
      781 tcgccgtcgc agagcgtgac ggtacaacat tgatgacgac aggttgcgct caaggcgtag
      841 aagccgagcg cgtcgtgggc gacgatggcc gcgttgttgt cgtcggcgcc gacgatcgtg
      901 gcccacccga ccggcagcga tgccgcgccc gcgaccgtca gccgctccag cccgacgagg
      961 cagccgatac ccggcccgtc ggacgggccc acgcacggcg gccgctgttt tgcactgtca
     1021 caaccgatcg tgccgaggcc gacgccgatg acgaggagct ggcgccgaga cagatgggga
     1081 tcgccgccgc tcaaggtgtg cacttgcctg acgcgacgtc gcacttgagc ggctgctggc
     1141 aatcctggtc gcgcgtgcac ggaaacgcgc acgcgtgatc ggtgggcccg aagccgatcg
     1201 gcggcgttgt gacaaacagt tggcatacgt acccctcggt cgttcgacag tcggtgtcgg
     1261 cggtgcaggc tttgaagcac ccgggcgttt cgcctcccaa ggccacgcag gtgccgccga
     1321 ccgggcaaac actcacgtcg ttgcacggct ccatgaagca atacccgccc ctgtagccgt
     1381 cgccggcttc ggtctggcac gcggaccgcg aatctgtgcc gcaatcgttg gtgctcgtgc
     1441 acggcgttcc gatcttgttc gtcgcatccg gcgcgcattt gccgccggcc tttgcggtgt
     1501 cgcagaccag tcccgacccg caggccgtgg tggcgctgca ggccgagccc agacctgaag
     1561 ccggcgctgg tccgtccttc gggcagtcga agagcagttc cagtgtgtcg atttccagcg
     1621 cgccctcgtc gctgaccgtc tggatcgccg tcatgtcgtt gctcggaacg gctgcggtga
     1681 gacgcgacag ggagtcgtcg atgtcggcta cgaccgtggc ggtcccgccc acgcggtgac
     1741 aggtcggcac gctcgcggcg acgctcggct tcgtcgtcgc ccaatcagcc atgaggctgt
     1801 cgagatcgtg ctggaccgcc gtgagatccc catagtgccc gtccagcccg acctgcctga
     1861 ataccgcgtc catccgcacg acgccgattg gcgcgccagt gtggaaatag tcgaacagct
     1921 caggcacggc aaggccaacc ttgttggaag cgaacgcggc cgcgcgctgg tccattccgg
     1981 gaattgcctg atcgagcgtc gccaccgcgt cgtcgaccat ctgtacctgc gtcgcgggga
     2041 gcttgggata cccggccttc gtgttgctcc agacttgctt caggaggacc aacacaccct
     2101 gagcgcgcgc ccaatcgggg aggtgtccgg tgggcgtgtc gccgaacgtc gtgttgacca
     2161 gtccctcccc gtcacgctcc acgtcccgca gagcggcggg cacaacggcg tcgtgagacg
     2221 acgagccgcc gcacgccccg accgcggggc cgagggcgac cgcaacggtc atccaaacgc
     2281 cgtacgagag cctccgtcgc cgcgtctccg cgctcacggc tccctgccgt tcgcgcacca
     2341 ggtggcatcg aacgttcccc gcagcgaacc actggggaac gtgagctgga agctgccggt
     2401 tgcgttcttg ttcgcgctga gggcggtgag agtaaccgtg ctccctgcgc tcgcgat
//
LOCUS       DASWVF010000589         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_195040, whole genome shotgun sequence.
ACCESSION   DASWVF010000589 DASWVF010000000
VERSION     DASWVF010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195040"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VGD55_10345"
     CDS             complement(<1..1240)
                     /locus_tag="VGD55_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0870786.1"
                     /translation="MTLFDDATRQAQAAVAPLAVRMRPRTLDEVVGQQQLRAPGTPLR
                     RMVENPDAGGSSVFLWGPPGTGKTTLAHVVSNATSRRFVELSAVNAGVKDVREALEEA
                     KRELGMSGRQTVLFIDEVHRFSKTQQDALLPAVENRLVTLIAATTENPFFSVISPLLS
                     RSLLLTLTPLTDDDIREVVDRALADERGLRGEYKLEADAQGQLIRMAGGDARRALTYL
                     EAAADGAGPARAITLEVLATAVDRAAVRYDRDGDQHYDVASAFIKSLRGSDVDASLHY
                     LARMIEAGEDPRFIARRLVILASEDIGLADPTALTTAVAAHQAVAFIGFPEASLTLAQ
                     AVIALAVAPKSNAVMTAISAALDDVRRGLIGSVPAYLRDTHYPGAAKLNHGSGYRYPH
                     DDAAGVVAQRYAPEPVADREY"
     gene            complement(1377..>1794)
                     /locus_tag="VGD55_10350"
     CDS             complement(1377..>1794)
                     /locus_tag="VGD55_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0870787.1"
                     /translation="SPKPEFAESFHEAPGEALAYAYDIVLNGSEIGGGSIRIHRGEMQ
                     QRVFDVIGLTKAEAESKFGFLLEAFTYGPPPHGGIAFGWDRVLTMLTGTESIREVIAF
                     PKTASGGDPLTGAPTTITPQQRKEAGIDFAPKPPAT"
BASE COUNT          294 a          622 c          586 g          292 t
ORIGIN      
        1 agtactcacg gtcggcgacc ggctcgggag cgtaacgctg cgccacgacg cctgccgcgt
       61 catcgtgcgg gtagcggtag ccgctgccgt ggttgagctt ggccgcgccc ggataatggg
      121 tgtcacgaag gtacgcaggc accgagccaa tgagcccgcg ccgcacgtcg tcgagcgccg
      181 cgctgatggc ggtcatcacc gcgttcgact tcggtgcgac tgcgagcgca atgacagctt
      241 gcgccaaggt caagctcgcc tcggggaacc cgatgaacgc caccgcctga tgggctgcga
      301 ccgcggtggt cagagcggtc gggtcggcga gcccgatgtc ttcgcttgcc aagatcacca
      361 agcggcgggc gatgaacctc ggatcctcac cggcctcgat catgcgcgct aggtaatgca
      421 ggctcgcgtc gacgtcgctg ccacgcagcg acttgatgaa cgcactcgcg acgtcgtagt
      481 gctggtcgcc gtcgcggtcg tagcgaaccg ctgctcggtc gaccgcggtg gccagcacct
      541 caagggtgat cgcccgcgcg ggtccagcac catcggcggc cgcttcgagg taggtcagcg
      601 cccggcgggc atcacctccg gccatccgga tcagctgccc ctgcgcgtcc gcctcgagtt
      661 tgtactcacc ccgcaacccg cgctcatcgg ccagtgctcg atcgacgacc tcgcgaatgt
      721 cgtcgtcggt aaggggtgtc agagtcagca ggagtgagcg cgagagcagc ggggaaatca
      781 ctgagaagaa cgggttctcg gtcgtcgctg cgatcaacgt gacgagacgg ttctcgacag
      841 cgggcagcag cgcgtcttgc tgggtcttgg aaaaccggtg cacctcgtcg atgaacagca
      901 cggtctgccg gcccgacatc ccgagctctc gcttcgcctc ctcgagagct tcgcggacgt
      961 ccttgacccc cgcgttcacc gccgacaact ccacaaaacg gcgggacgtc gcgttgctca
     1021 cgacgtgcgc aagcgtcgtc ttcccggtgc caggcggacc ccacaggaac accgaactac
     1081 cgccggcatc agggttttcg accattcgtc gcagcggcgt gccgggcgca cggagctgct
     1141 gctgaccgac cacctcgtcg agggtgcgcg gccgcatccg gaccgcgagc ggcgcgacgg
     1201 cagcctgcgc ttgccgggtc gcgtcgtcga acaatgtcac cctgcgcccc tccgggtcga
     1261 cgtcacagca ccgaccctac ctagggcggc caccactcgg cgtccggtca aagtctgcgg
     1321 tgcccgccgc taacgtggag cgccggcgtt gttctagcga cgacgagact ccacgatcag
     1381 gtggccggcg gcttcggcgc gaaatcgatg ccggcttcct tgcgctgctg gggagtgatc
     1441 gtggtcggcg cgccggtgag cgggtcaccg cccgacgccg tcttcggaaa cgcgatcacc
     1501 tcgcggatcg actcggtacc ggtcagcatc gtgagcacgc ggtcccaccc gaacgcgatg
     1561 ccgccgtgcg gaggcggccc gtacgtgaac gcctcgagca ggaagccgaa cttcgactct
     1621 gcctccgctt tcgtcaggcc gatcacatcg aacacccgct gctgcatctc accgcgatgg
     1681 atacggatcg agccaccgcc gatctcgctg ccgttcagca cgatgtcgta cgcgtacgcg
     1741 agcgcctccc ccggcgcttc gtgaaacgac tcagcgaatt cgggcttcgg tgac
//
LOCUS       DASWVF010000590         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_195056, whole genome shotgun sequence.
ACCESSION   DASWVF010000590 DASWVF010000000
VERSION     DASWVF010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195056"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1027)
                     /locus_tag="VGD55_10355"
     CDS             complement(8..1027)
                     /locus_tag="VGD55_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870788.1"
                     /translation="MMDVEGLLRETLNDPRRRLEPVPGMFDAVSERARQRRQRGLRLA
                     SAMTVVVLIAGVATAIGIGTQNHTKVAAPASITPSPQTGQATPINLGVGSTTSFALAP
                     SGIFVGRTEPSQLLQVSFAEASVIKRTATTDDIDGIAADSVEGLVWTWSSSQPIVSDT
                     STDVGSTIVRARTTLPGTVTIDAPAAVPAYTFSAAALDGDLWLATSDGLFVVALDQAS
                     WSVTKLVSGSVFSIAADAVRDRILYGSAVGTTDAGSNSLGSIAVHEINARTRTAIAVG
                     TPLPIGKESIAIVGDQIWVAGYGSGSTPRVFHLDGSSLEPIATSDLNLGSPLELGPGA
                     RLRSQ"
     gene            complement(1024..1527)
                     /locus_tag="VGD55_10360"
     CDS             complement(1024..1527)
                     /locus_tag="VGD55_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870789.1"
                     /translation="MNRADEDDFRAFVAARSSALLWFAHLLTGDRNAAEDVVQTALAK
                     TALGWSRVRRKDNPEGYVRRAIVNTHLNAVRRKPWREQPHEYIPEDAEVRRPEGELDD
                     RDAMWHALAELPPRQRAVLVLRYYEDLSESDIADVLGCSRGTVKSQAAKGLVHLRRIV
                     QKEDASP"
     gene            1910..>2411
                     /gene="rpsP"
                     /locus_tag="VGD55_10365"
     CDS             1910..>2411
                     /gene="rpsP"
                     /locus_tag="VGD55_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0870790.1"
                     /translation="MAVKIKLMRLGKMRAPYYRIVVADSRTKRDGRAIEEIGKYHPKE
                     DPSFIEVKGDRAAYWLSVGAQPTEAVTAILKVSGDWQKFKGLPAPPPMLVAKPKADKK
                     IAFEAAAKEGSGEPTTDATTPKSRRAPAKKTADPSSPESSDAAAVAVEAEEAEAGGVE
                     AITAVQS"
BASE COUNT          424 a          781 c          818 g          388 t
ORIGIN      
        1 cgagccgtca ctggctgcgt aagcgtgccc ccggaccaag ttcgagcggg ctgccgaggt
       61 tcaagtcgct cgtcgcaatg ggttccaacg acgacccatc aaggtggaac acccgaggag
      121 tggaacctga cccgtagccg gcgacccaga tctgatcgcc gacgatcgcg atcgactcct
      181 tgccgatagg gagcggggtc ccgacggcga ttgcggtgcg ggtgcgtgcg ttgatttcgt
      241 ggacggcgat gctgccgagg gagttcgagc cggcgtccgt cgtaccgacc gcactgccgt
      301 acaggattcg gtcacgcacg gcgtcggcgg cgattgagaa cactgacccg ctgacgagct
      361 tcgtgacgga ccacgatgct tggtctaggg cgacgacaaa taggccatcg gatgtcgcga
      421 gccacaggtc accgtccagc gccgcggccg agaaggtgta ggccggaacg gccgctgggg
      481 catcgatcgt cactgtgccc ggcaacgtcg tacgggcacg gacgatcgtg gagccgacgt
      541 cggtcgaggt gtccgacacg attggttggc ttgacgacca tgtccacacg aggccctcaa
      601 ccgagtcagc agcgatgccg tcgatgtcgt cggtcgtcgc ggtgcgtttg atgaccgacg
      661 cctccgcgaa ggaaacctgg aggagttgcg aaggttcggt ccgcccgacg aagattcccg
      721 atggcgcgag ggcaaacgag gttgtgctgc ccacaccgag gttgatcggc gtcgcttggc
      781 cggtctgcgg cgacggcgtg atcgacgcag gtgctgcgac cttggtgtgg ttctgcgttc
      841 cgataccgat cgcagtcgcg acgccggcga tcagcacaac gacagtcatc gcgctggcaa
      901 gccgcagacc ccgttgccgt cgctggcgcg ctcgctcgct cacggcgtcg aacatccccg
      961 gcaccggctc aagacgccgt cgcgggtcat tcaatgtctc gcgcagcaac ccctcgacgt
     1021 ccatcatggc gatgcgtcct ccttttgcac gatccgccga agatgaacca atcctttggc
     1081 ggcctggctt ttcacggttc cccgactgca accgagcaca tcggcgatgt cggattcgga
     1141 gaggtcttcg tagtaccgga gcaccagcac ggctcgctgc cgtggcggca gttctgcgag
     1201 tgcgtgccac atggcgtcgc ggtcgtcgag ctcgccctcg gggcggcgca cctcggcgtc
     1261 ctcggggatg tactcgtgcg gttgctcacg ccacggcttg cgacgcaccg cgttcaggtg
     1321 cgtgttgacg atcgcgcggc ggacatatcc ctcggggttg tccttccggc gcacccgcga
     1381 ccagccgagc gctgtttttg cgagcgcggt ctggacgacg tcctccgcgg cgttgcgatc
     1441 acccgtgagc aggtgtgcga accagagcag ggccgaggag cgcgccgcga cgaacgccct
     1501 gaagtcgtcc tcgtcggcgc ggttcaccgg ctagctccca tcggggtcat gtacctaagg
     1561 acacggtctc gcgcgcgccg gttgcccacc agcccgaaga gaggcgcgag ccacttcaca
     1621 tccggcgcgg tcgtaaagcg ggctgaattg cgcgggattc acgaccacac cggatgtgaa
     1681 gacggggagg cgagacgcga gggcgcaggg acggcggggt ggtcggcgtc tcgcgaaagc
     1741 tcgccgatgt gcgtcctcgg cggtcggtct ggcagaatgg cgggtcgaac accttccggc
     1801 ggaggccctc taccctgcgc tggtcgtgtc ctcgaggcgt cgcgaccggg tccccaccac
     1861 gtcgatcgac ggctcacctg tcaaacatga gttggagcac cgaagtcccg tggcagtcaa
     1921 gatcaagctc atgcgcctag gcaagatgcg cgcgccgtac taccggatcg tcgtcgccga
     1981 ctcgcgcacc aagcgcgacg gcagggcgat cgaggagatc ggcaagtacc acccgaagga
     2041 agatccgtcc ttcatcgagg tcaagggcga tcgcgccgcg tattggctca gcgtcggcgc
     2101 ccagccgacc gaggcggtca ccgcgatcct caaggtcagc ggcgattggc agaagttcaa
     2161 gggcctgccc gcgccgccgc cgatgctggt tgccaagccg aaggctgaca agaagatcgc
     2221 cttcgaagct gccgccaagg aaggctcagg cgagccaacc accgacgcga caacgccgaa
     2281 gtcgcgccgg gcccctgcga agaagaccgc cgacccgagt tcgcccgagt cgtcagacgc
     2341 cgccgctgtc gccgtcgaag ccgaagaagc cgaagctggt ggagtcgagg caatcaccgc
     2401 cgtgcaaagc g
//
LOCUS       DASWVF010000591         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_144059, whole genome shotgun sequence.
ACCESSION   DASWVF010000591 DASWVF010000000
VERSION     DASWVF010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144059"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VGD55_10370"
     CDS             complement(<1..773)
                     /locus_tag="VGD55_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870791.1"
                     /translation="MSSTSHDFATRLSAELHQSLDAVPVDDLAWFDAQPKANERAGRR
                     RRRARMRMTATAVAATVAVAVVVAQAGTRRTSRPATPSRPQTFQVVATADVGGATPAV
                     LAAGGGSVFAGLWDSGQLLRIDPITLRTTATLQVGTPRSGPLSISYGDGAVWVLNFDD
                     SQLWRVDPATMTPTLRVPLPAQPSQVAFGDGAVWVTVCCSSTETATRQRLLRIDPTTG
                     TITGTHQVAGEGETVPLAVGPDVVVTTQNGPVDVIDPNT"
     gene            complement(770..1294)
                     /locus_tag="VGD55_10375"
     CDS             complement(770..1294)
                     /locus_tag="VGD55_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870792.1"
                     /translation="MGDNDAGPEPGRDELVAVLYHRHHRELVRLAYGLLGDRGEAEEV
                     VQDAFAALLSRWSSVRDIQAAPGYLRTAVLNGVRGRWRRRRVRALADRTLRRDPASKE
                     SDVVEQAVVLAALARLPLRKRECVLLRYYADLSEIETAAVLGISVGTVKSQTSKALTQ
                     LAAFLEPERAEVTQ"
     gene            1369..2235
                     /locus_tag="VGD55_10380"
     CDS             1369..2235
                     /locus_tag="VGD55_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0870793.1"
                     /translation="MQIGVVYPQTELRGDPVAVRRIGKAVEDLGFDYLLAYDHVLGAV
                     HADRTPALTGPYTERHPFHDPLVMFGYLAGITERIGFATGVLILPQRQTALVARQASD
                     VDLLSGGRLRLGVGIGWNPVEYEALGQDFHTRGVRQEEQIQLLRRLFTEPVVDFTGRF
                     DRVDRAALVPKPTRSIPIWLGGYGEKAFDRAARLGDGFIFFGGSGVEHAIENLTWLHS
                     RLRDVGRSVEDFGAEFVALPQSIEHLRADVDRWRDAGGTHLSIVTMGQGLDSIDAHID
                     FLATSAAALSLS"
BASE COUNT          394 a          798 c          746 g          393 t
ORIGIN      
        1 atggtgttcg gatcgatgac gtcgaccgga ccgttctggg tggtgaccac tacgtctggc
       61 ccgacggcga gcgggaccgt ctcaccctca ccggcgacct gatgcgtgcc ggtgatcgtt
      121 cccgtcgtcg ggtcgatgcg caacagtcgc tggcgggtcg cggtctcggt cgaactgcag
      181 cacaccgtca cccacacggc gccgtccccg aaagcgacct gagagggctg agcgggcaac
      241 gggaccctca aggttggggt catcgtcgcg ggatcgaccc gccaaagctg actgtcatcg
      301 aagttcagaa cccataccgc gccgtccccg taactgatcg acagcggccc gcttcgcggc
      361 gtgccgacct gcagagttgc ggtagtacgc aaggtgatcg gatcgatgcg caggagttgt
      421 cccgaatccc acagcccggc gaatactgag ccgccacccg cagccaatac cgctggggtg
      481 gctccgccga catcggcggt cgccacaacc tgaaacgtct gcggtcgact cggagtcgcc
      541 gggcgcgacg tgcgtcgggt gcccgcctgc gccaccacga cggcgacggc cacggtcgct
      601 gcgaccgccg tcgcggtcat cctcatccgc gcgcgcctgc gtcggcgtcc ggcacgttcg
      661 tttgctttcg gttgcgcgtc gaaccatgcg aggtcgtcaa cgggcacggc gtctagtgat
      721 tggtgcaact cggcactaag ccgggtggcg aagtcgtgcg atgtgctgct cattgggtca
      781 cctctgctcg ctcgggctcg aggaacgccg ctaactgcgt gagcgccttc gaggtttgac
      841 tcttcacggt gccgacgctg atcccgagca ctgcggcggt ctcgatctcc gacaggtcgg
      901 cgtaatagcg cagcagcacg cactcgcgtt tgcgtaacgg caggcgagca agtgccgcaa
      961 gcaccacagc ctgctcgacg acatcgcttt ccttggacgc cgggtcccga cgcagcgtgc
     1021 ggtcggcaag ggcgcgtacc cgccggcggc gccaacggcc gcggacgccg tttagcactg
     1081 ccgtccgcag atagccggga gctgcctgga tgtcacgcac ggatgaccac cggctcaaca
     1141 acgcggcgaa cgcgtcctgc accacctctt cggcctcgcc tcgatcgccg agcaagccgt
     1201 acgcaagccg aacaagctcg cggtgatgcc ggtggtacag caccgcgacc agctcatcac
     1261 ggccaggctc gggcccggcg tcgttatcgc ccacgagcaa caagagtcct cggccctgcc
     1321 aaaggttgcc agcgcgcagc ctcgggatct cgtggcaggc tgctgggtat gcagattggc
     1381 gttgtctatc cccaaaccga gcttcgcggc gatcccgtcg ctgtccggcg aatcgggaaa
     1441 gcggtcgaag acctcggctt cgactacctg ctggcttatg accatgtgct cggcgcagtt
     1501 cacgccgatc gaacgcctgc actgacgggt ccgtacaccg agcgccaccc gttccacgac
     1561 ccactcgtga tgttcggcta tctcgcgggg atcaccgaac gaatcggttt cgcgaccggg
     1621 gtgttgatcc ttccgcaacg acagacagcg ctggtcgctc gccaggcatc cgacgtcgat
     1681 ctgctgtcgg gcggccggtt gcgccttggt gtcggcatcg gctggaaccc agtcgagtac
     1741 gaagcgctcg ggcaggactt ccacacccgc ggcgtccggc aagaggaaca gatccaactg
     1801 ctgcgccggc tgttcaccga acccgtcgtc gacttcaccg ggcgcttcga ccgcgtcgac
     1861 cgcgcagcgc tcgtgccgaa accgacgcgc tcgatcccga tttggctcgg cggatacggc
     1921 gaaaaggcat tcgaccgggc cgcccggcta ggcgacggct tcatcttctt cggcggcagc
     1981 ggcgtcgaac acgccatcga aaacctgacc tggctgcaca gtcggctacg agacgtcggg
     2041 cgatcagtcg aagacttcgg tgcggagttc gtcgccctcc cgcagagcat cgaacatcta
     2101 agggcggacg tcgaccggtg gcgcgatgcg ggcggcactc acctgtcgat cgtcacgatg
     2161 ggtcaaggcc tcgactccat cgatgcccac atcgacttcc tggcgacgag tgcagcggcg
     2221 ctcagtctgt cgtgaccagc tgcgacaggc ttcccgcgtg aggcaccgcg atccatccct
     2281 cacgcctggc caactcgatc gcatcgcccg tcgggggagt agtgaggcca c
//
LOCUS       DASWVF010000592         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_93015, whole genome shotgun sequence.
ACCESSION   DASWVF010000592 DASWVF010000000
VERSION     DASWVF010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93015"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3511
                     /locus_tag="VGD55_10385"
     CDS             <1..>3511
                     /locus_tag="VGD55_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0870794.1"
                     /translation="ATTFTMPAANNTTLKVTDDTDHTCDDGFQYTVQITSAAPDDTVV
                     SLYAGNLLLRTTTVTGGTASFPVVLSTGSTAESLSIQYPSTTACSVTSTVTVDCPNTP
                     PSCTISAPVISATHPALNGIATPAGDRVSSVGSPYQATFKVQTSAEDGQPVTLAVNNV
                     APGSATTMLNATASAGVATFTVTLVPDATYAAAATCTNRNGISGSSSKGTFLVDTTAP
                     DLTVNSPSAGQFIVAPVVHVCGQTDSPDATALSSSLGASQNNFCVTVGSAATPSCAPV
                     TAVNSPACIDIPCPGAAPFALTIALKDSAGNPTSQTLTGLTCVSALPTVQIISPLSGS
                     PGFTDKTKNILAATAPTGVKDLDANTAGAQVNVVACTDAVGTAVLTVGHVGDATLAQL
                     GSSVPTAAAVAGDNCPAGLPNVVKFPGVTLPQSTEGADGTLVAATRLIVSVTSAANTL
                     ATGVSPADDVWIDTVPPAVGLAGPSDFCSFKQSSQATVPQSPEFTADDSLVVLDVTNG
                     AVTTSYDTPVSYMSGVAQFANVILEQGQNTVTVTNSDPAGNSTSVNVCTVTVGMAPVV
                     TFTTPTAGAILCPAGATSTACIPDNNAGTAGWQGSLAVTVTAGSTNTAVTTGDLVTFS
                     IGGNSLGSANLDGSGHAQLNGITIPDGVQTVVATTAAITNSGVGAGSVTVTVDTTPPA
                     APTGLNAAVLDRRKTTMQLTWTAPSDGVSANVAGYQVRYAKVPIDSTNFDNPAVTTAV
                     AYTGAPSTPGHVDGVTVSGLYIENGYYFAVEAVDVAGNISPILASPASGTCTCAGQCC
                     AAHFNMTTVASGLGLSDEQFGNQLDGTGDVNGDGLSDIIVGTTDGQHAYLYLGTSGTF
                     SPSAPAVTFSGDGTTVLFGYGVAQIGDIDADGREDIAISDPGSLKVFIYKGRSVWPLT
                     MGPADANYVVSTDASYVGSAFATSIARLGDFNGDGVDDFAIGADGYKVGGFSSVGRVI
                     IVLGAAGFGGVALPSATGAITIDGDSSLTKPLFGAQVLGLGHFYSVTTGTTLVVSSPG
                     LSSSANSSQGRLYAFHGQPGTAGAISIATADSVVVGPVNKTDMGSVLSNLGPLVNGLP
                     SVASGNPLDAVSTSGISGSVFLFSGDTTAGVFANRITLSRSGASRNGEVMLGGAVSGR
                     NSTYSLLGDSHADLVLAAQTAANI"
BASE COUNT          638 a         1160 c         1078 g          635 t
ORIGIN      
        1 cgcgacgacg ttcacgatgc cggccgcgaa caacaccact ttgaaagtca ccgacgacac
       61 cgatcacacg tgtgacgacg ggttccagta caccgttcag atcaccagcg ccgctccgga
      121 cgacaccgtc gtttcgcttt atgccgggaa tttgctgctc aggaccacca cggttacggg
      181 cgggaccgca tcgttccctg tggtgctttc cacgggcagc acggccgaat cgctttcgat
      241 ccagtacccg agcacgaccg catgctccgt gacgagcacc gtcacggtgg actgcccgaa
      301 taccccgccc agctgcacga tcagcgcccc ggtgatcagt gcgacccacc cggcattgaa
      361 cggcattgcg acaccggccg gcgaccgcgt cagctcggtc ggatcgccgt accaggcgac
      421 gttcaaggtg cagaccagcg ccgaggacgg ccagccggtc actctggcgg tgaacaacgt
      481 tgcgccaggg agcgcgacga caatgctgaa cgcgacggct tccgctggcg ttgcaacatt
      541 caccgtcacc ctggtccccg acgccaccta cgcagcggcc gcgacctgca cgaacaggaa
      601 tgggattagt ggcagttcga gcaaggggac gttcctagtc gacacgacag cgcccgacct
      661 gacggtgaac agccccagcg ctggccagtt catcgtcgcg ccagtcgtgc acgtctgcgg
      721 gcagacggac tcgccggatg cgaccgcgct gtccagctcc ctcggagcca gtcagaacaa
      781 cttctgtgtc accgttggat cggccgccac gccgagctgc gcgccggtga cggccgtcaa
      841 ctcgcccgct tgtatcgaca ttccctgccc cggcgctgcg cccttcgctt tgacgatcgc
      901 gctgaaagac tccgcgggga acccgacgtc gcagacgctg accgggctca cctgcgtgtc
      961 tgctctgcct accgtccaga tcatctcgcc gctttcgggc tcgcccgggt ttacggacaa
     1021 gaccaagaac atcctggctg ccaccgcgcc gacgggcgtc aaggacctgg acgccaatac
     1081 tgcgggcgcg caggtcaacg tcgtcgcgtg caccgacgcc gttgggaccg cggtcctcac
     1141 cgtcggtcac gtgggtgacg cgacgctggc gcagctcggc agctccgtcc cgaccgcagc
     1201 ggcggtggcg ggcgacaatt gccccgccgg tctgccgaac gtcgtgaagt tccccggcgt
     1261 cacgctgccg cagagcacgg agggcgctga cggcaccctg gtcgcggcca cccggttgat
     1321 cgtctccgtg acgtcggccg cgaacactct ggccactgga gtcagcccag ccgacgacgt
     1381 ctggattgat accgttccac cggcggtcgg gctggccggc ccaagcgatt tctgcagctt
     1441 caagcagtcg tcgcaggcga ccgttcccca gtcgccagaa ttcaccgccg acgattcctt
     1501 ggtggtgctg gatgtgacga acggagccgt gacgacgagc tacgacacgc ccgtgtccta
     1561 catgtccggc gtcgcccagt tcgcgaacgt catcctggag caggggcaaa acaccgtcac
     1621 cgtcacgaac agtgacccgg cgggcaactc gacgtccgtc aacgtctgca ccgtgacagt
     1681 cggtatggcg cccgtcgtca ccttcacgac cccgaccgcg ggcgcgatcc tgtgcccagc
     1741 cggcgcgacc agcaccgcgt gcatccccga caacaacgcg ggcactgccg gctggcaggg
     1801 tagcctggcc gtgactgtga ccgcgggttc caccaacacc gccgtcacga ccggcgacct
     1861 cgtgacgttc tcgatcggcg gtaactcgct cggcagcgcg aacctggacg gctccggcca
     1921 cgcccagctc aacggcatca ccatccccga tggcgtccag accgtcgtcg ccacaacggc
     1981 agcaatcacc aactcgggtg tcggagctgg cagcgtgact gtcacggtcg ataccacccc
     2041 acccgctgcg ccgacggggt tgaacgcggc ggttctggat cgccggaaaa cgacgatgca
     2101 attgacttgg actgctccca gcgacggcgt ttccgcgaac gtcgctggct atcaggtgcg
     2161 gtacgccaag gtcccgatag attcgacgaa cttcgacaac cccgcggtaa cgaccgccgt
     2221 tgcgtacacg ggagcgccgt caactcccgg ccacgtcgac ggggtgacgg tgagcggtct
     2281 ctacatcgaa aacggttact acttcgcggt cgaggccgtt gacgtcgcag gaaacatcag
     2341 cccgatcctc gccagtccag caagcggcac ctgcacgtgc gcgggccagt gctgcgcggc
     2401 acatttcaac atgacgaccg ttgcgagtgg tcttggtttg tctgacgaac agttcggtaa
     2461 ccaactcgac ggcaccgggg atgtcaatgg agacggcttg tccgacatca ttgtcggtac
     2521 caccgacggg caacatgcat acctgtatct cggtacgtcg gggaccttct cgccctcggc
     2581 cccggcagtg acattctctg gtgacgggac gacggtcctt ttcggatacg gtgtggccca
     2641 gatcggagac atcgatgcgg acgggcgaga ggacatcgca atatcggacc cgggatcgct
     2701 gaaggtcttc atctacaagg gcaggagtgt ttggcctttg acgatggggc ccgccgacgc
     2761 gaactacgtc gtctctacag atgcctcgta tgtagggtct gcatttgcaa cgtccatagc
     2821 ccgactgggt gacttcaacg gcgacggcgt cgatgacttc gcgatcggcg cagacggcta
     2881 caaggtcggt ggtttctcca gcgtggggcg agtcatcatc gtcctgggag cggccggctt
     2941 cgggggggta gctctgccca gcgcgaccgg tgcaatcacc atcgatggtg attcttcgtt
     3001 gacgaagccg ctgttcggcg cgcaggttct cggattgggc catttttatt cggtgaccac
     3061 cgggacaacc ctcgtcgttt cgtcgcccgg cctctcgagc tctgcgaaca gcagccaggg
     3121 gcgcctttac gccttccatg gtcagccggg cactgccggc gcaatttcta ttgccaccgc
     3181 tgacagcgta gtcgtcggtc ctgtcaataa gacagacatg ggcagcgtgc tgtcaaattt
     3241 aggccccctg gtcaacggct taccctcggt tgcgagtgga aatccgttgg atgcggtttc
     3301 cacatccgga atcagcggaa gcgtattttt gttctcgggc gatacgactg caggcgtgtt
     3361 tgcgaatcga atcacactga gtcgttctgg tgcgagtcgc aacggagagg tcatgttggg
     3421 tggcgcagtt tccggccgaa attcaacata ttcgttgctg ggtgattcac atgcagactt
     3481 ggtgctagct gctcaaacag cggcgaatat t
//
LOCUS       DASWVF010000593         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_450060, whole genome shotgun sequence.
ACCESSION   DASWVF010000593 DASWVF010000000
VERSION     DASWVF010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450060"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..448)
                     /locus_tag="VGD55_10390"
     CDS             complement(50..448)
                     /locus_tag="VGD55_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HEY0870795.1"
                     /translation="MASPRPEDVAALVRSSAGDGVWLPVDGSSMLPTISAGSQVRVVS
                     VSRRPRIAEIWAFCDPNGEIIVHRYRRQDHAQRFVFRGDNVKQSDMPVDAERLIGVVR
                     VIDANGTKRAAGWLARVRWIAHRAGARIRR"
     gene            462..1352
                     /locus_tag="VGD55_10395"
     CDS             462..1352
                     /locus_tag="VGD55_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870796.1"
                     /translation="MRLVVAEPALVVEVDRLFAPMPDHDGPYAAEIVFQAQAPAVPDR
                     APDEVYEPILLWRAGDELFLDSGGPLRALATPTHVVVGGVAGDGALGALLLRRIVHHV
                     IAHVLSLSGRLVAHGAAVGRHGQGILMLGASGSGKSSSSYLASLAGWSLLSDDLVVVA
                     NGSNGLELIGVHRAIAVPPDVIEAEAADIEHDLRDRRRPDVVLDARPHRLAAVALVGH
                     GGEQGSVDQVSGLAVASAFLGSTPAAGNTDVAAEALRTAAALAMFPCFRLLLPDTARA
                     RLDETGGLFERIAESAGIPR"
     gene            complement(1403..1669)
                     /locus_tag="VGD55_10400"
     CDS             complement(1403..1669)
                     /locus_tag="VGD55_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0870797.1"
                     /translation="MTYRRNPAVVFQHVDDRVMMMNPDRAEFVTVSPTGAQVWDALET
                     PGTADDIVQRLLPKWPGVDPEQMLKDIDTFLVKLEEAGAVFVEK"
     gene            complement(1776..2204)
                     /locus_tag="VGD55_10405"
     CDS             complement(1776..2204)
                     /locus_tag="VGD55_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0870798.1"
                     /translation="MLDETSPVFVISVAAELSGLHPQTLRTYDKLGLVSPDRAVGRGR
                     RYSSRDISQLREVQRLSQEHGVNLAGIKRILELENQVTALRQRLAELSYELDAVHASA
                     ARQVAEAHASHRRDLVPITQMTATVVWRPGTPGPRLGGTS"
     gene            complement(2207..3388)
                     /gene="dnaJ"
                     /locus_tag="VGD55_10410"
     CDS             complement(2207..3388)
                     /gene="dnaJ"
                     /locus_tag="VGD55_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031072 - heat shock protein binding
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0870799.1"
                     /translation="MSTRDYIEKDYYKSLGVPKDATAAEIKKAYRKLARVHHPDANAG
                     GGAKSEEKFKEISEAYDVLSDQRSRKEYDEARSLFGGGGFRMPGSSGGANGAPGGFNF
                     DLGDLLGNARAGGAGAAGGIGDILGGIFGGGGRRPNTTQQPRRGADVETEVTLDFEHA
                     VRGETVALRMATPHPCTACNGTGAKAGTTPRVCPTCSGTGVTSRGAGGFAFSEPCRDC
                     RGRGLIVDDPCPVCHGSGRAMSEHTMQVRIPAGVADGQRIRLKGKGSPGERSGPNGDL
                     IVLVHVRPHELFGRAGDNLTITLPVTFPEAALGAEVKVPTLDGPTVTLKLPPGTTNGR
                     VLRVRGKGVPRRDGTKGDQLVTIEVAVPQKVSGKAKEALEAYRIATASDNPRAHLPGG
                     G"
     gene            complement(3416..4051)
                     /gene="grpE"
                     /locus_tag="VGD55_10415"
     CDS             complement(3416..4051)
                     /gene="grpE"
                     /locus_tag="VGD55_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0870800.1"
                     /translation="MSGSDPFDQPIVRDKRRVHFDPASGAVSQDPRSGEGPASAGASD
                     PGSRGGVDSVELSQLRIQVAERTADLQRLQAEYANYRKRVERDREAVREFAVAEALAA
                     LLPVLDDIGRAREHGELEGGFRQVAEALDAAVTKLGLTRFGQPGDPFDPTQHEALMHS
                     YSDEVSEPTAVQILQSGYSYAGRVIRPARVAVAEPTAALPADETVEEPSDQ"
     gene            complement(4048..5913)
                     /gene="dnaK"
                     /locus_tag="VGD55_10420"
     CDS             complement(4048..5913)
                     /gene="dnaK"
                     /locus_tag="VGD55_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0870801.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGEPTVIANAEGSRTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNADRTIRSVKRHIGTDWAVTIDGKKYTSQEISARILQKLKRD
                     AEAYLGEQITDAVITVPAYFNDSQREATKEAGTIAGLNVLRIINEPTAAALAYSLDKG
                     EKEQTILVFDLGGGTFDVSLLEIGEGIVEVKSTSGDTHLGGDDWDQRLVDFLVTAFKN
                     SNGIDLAKDKMALQRLREAAEKAKIELSQQAETTVNLPYITASAEGPLHLEVKVTRSE
                     FQKMTSDLLDRTKGPFHQAIKDAGIKVDQIDHVVLVGGSTRMPGVVDLVRELTGGKEP
                     NKGVNPDEVVAVGASLQAGVMKGEVKDVLLLDVTPLSLGIETKGGVFTKLIERNTTIP
                     TKRSETFTTADDNQPSVQIQVYQGEREMAAYNKKLGMFELTGLPPAPRGIPQIEVTFD
                     IDANGIVHVSAKDLGTGKEQSMTITGGSALPKDDIERMMREAEAHAEEDRARKEDAET
                     RNQAETLVYQTEKFLRDNAESVPAPERDAVDTALAGLKKALEGSDFAAIRTATDQLAA
                     ESQKMGSAIYANAQANSANSADAGHADHEHSASEEGSTDGADEVIDAEIVDEGKS"
     gene            6245..>7249
                     /locus_tag="VGD55_10425"
     CDS             6245..>7249
                     /locus_tag="VGD55_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870802.1"
                     /translation="MTRRLLHGRPFRFFSGGVVAITLMGGGSIVTATAASAVTPVPSN
                     VHRIAGSTRLATAIGASQDQFPTAGSASAVVLARSDTFPDALAGGPLAAKVHGPLLLT
                     SSASLDPAVSVEILRVLPAGGTVYILGGTAAVSAAVATTLTANHYVVIRLSGADRFAT
                     AVAVADQMGDPTTVFEATGTNFPDALAGGPAAIKTGGVILLTNGATQSTATAAYLAAH
                     PGGTHYALGGPAAAADPVATPLMGDDRFWTSIAVAGTFFPDATTVGAATGLNFPDALA
                     AGSDLAAKGAPLLLVPTAGALPTAIAVDLLNRSFTGSNSVLLFGGTASVSDDVATQLG
                     V"
BASE COUNT         1079 a         2426 c         2385 g         1359 t
ORIGIN      
        1 tcccgccgtc cgtacgcctg aattcgcgct gtttcgccga tcagccttgc taacgccgta
       61 ttcgcgcccc cgcccgatgg gcgatccagc gcacgcgtgc aagccaccca gccgcccgct
      121 tcgtgccgtt cgcgtcaata accctgacca cgccgatgag ccgctccgca tcgaccggca
      181 tgtcgctctg cttgacgttg tcgccgcgaa acacgaatcg ctgcgcgtgg tcctggcggc
      241 ggtaacggtg gacgatgatc tcgccattgg ggtcacagaa cgcccagatc tctgcgatcc
      301 tcggacgccg ggagacactg acgacccgca cctggctgcc ggcagaaatc gtggggagca
      361 tggacgaacc atcgacgggc aaccacacgc cgtcccccgc actcgatcga acgagcgcgg
      421 cgacatcttc gggtcgcggg cttgccatcg ctggccttcc tgtgaggctg gtggtcgccg
      481 agccggcgct cgtcgtcgag gtcgaccggc tcttcgcgcc gatgcccgac cacgacggac
      541 cttatgccgc cgagatcgtc ttccaggcgc aggcgcccgc agtgcccgac cgggcaccgg
      601 atgaagtcta cgaaccgatc ctgctgtggc gcgccggtga tgagttgttc ctcgacagcg
      661 gaggtccgct gcgcgcgttg gccaccccga cgcacgttgt cgtgggtggt gtcgcgggtg
      721 acggcgccct cggtgcgctg ctacttcgcc gcatcgtgca ccacgtgatc gcccacgtcc
      781 tcagtttgtc cgggcgcctt gtcgcgcacg gcgcggcggt cggccggcac gggcagggca
      841 tcttgatgct cggtgcgagc gggtcgggga agtcgtcgtc gtcctacctg gcgagcctcg
      901 ccggctggtc gttgctcagc gacgatctgg tggtcgtggc gaacgggtcc aacggactcg
      961 agctgatcgg cgtccatcgt gcaatcgctg tgccgcctga cgtgatcgag gccgaagccg
     1021 ccgacatcga gcacgacctt cgcgaccggc gtcggcccga cgtcgttctc gatgcgcgcc
     1081 cgcatcggct ggccgcagtc gcgctggtag ggcacggtgg cgagcagggc agcgtcgatc
     1141 aggtctcagg actcgcggtc gcgagcgcat ttctcggctc gacgcctgcc gcgggcaata
     1201 ccgatgtggc ggccgaggcg ttgcgaacag ccgcggccct cgccatgttc ccgtgctttc
     1261 ggctgctgct gccagacacg gcgcgcgccc ggctcgatga aacaggcggc ttattcgagc
     1321 ggatcgccga atcggcaggc attccgcggt agacacgcag agacgggcat ttcaagcatg
     1381 ggcggacgcc gcgggcagcc gactacttct cgacgaagac cgcacccgct tcttcgagct
     1441 ttaccaagaa ggtgtcgatg tccttgagca tctgctcggg atcgacgccg ggccatttcg
     1501 ggagcagccg ctgcacgatg tcgtcggccg tgccgggggt ctcgagtgca tcccacacct
     1561 gggcgccggt ggggctcacc gtgacgaact ccgcgcggtc aggattcatc atcatcaccc
     1621 ggtcgtcgac gtgctggaac acgacagccg ggttgcgccg atacgtcacc gtggtgcggc
     1681 cctccaggtc ggttcgtaca ggtatcgagc gtcacgggat ggcgcttgag cgcgcccatc
     1741 atgccgttga agtgccgtcc ccgcgactct tgagcctacg aggtgcctcc gagccgcggc
     1801 ccgggagttc cgggccgcca gacgaccgtg gcggtcatct gggtgatcgg aacaagatca
     1861 cgccggtggg aggcgtgtgc ctcggccacc tgacgggcgg cgctcgcgtg cacagcgtcg
     1921 agttcgtagg agagctcggc gagccgctgc cgaagggccg tgacctggtt ctcgagctcg
     1981 aggatccgct tgatcccggc cagattgacg ccgtgctctt gcgacagtcg ctgcacttcg
     2041 cgcagctggg agatgtcgcg gcttgagtac cgccgacctc gcccgaccgc gcggtcgggc
     2101 gacaccaaac cgagcttgtc gtacgtgcgc agcgtctggg ggtgcaggcc cgagagctcc
     2161 gcagccaccg agatcacgaa gaccggactg gtttcgtcga gcatgactac ccgcctcccg
     2221 gcagatgcgc tcgtggatta tccgacgcgg tcgcaatgcg gtaggcctcg agcgcttcct
     2281 tggccttgcc ggaaaccttc tgcggtaccg cgacctcgat ggtgacgagc tggtcgcctt
     2341 tggtgccatc gcgacgtggc acgcccttgc cacgaacgcg caacactcgc ccgttggtgg
     2401 tgccaggcgg cagtttgagg gtcacggtcg ggccgtcgag ggtcggcact ttcacctcgg
     2461 cgccaagtgc cgcttcgggg aaggtcacgg gcaacgtgat cgtgaggttg tcacccgccc
     2521 ggccgaacaa ctcgtgcggc cgcacatgca ccaagacgat caggtcgccg ttcgggccgc
     2581 tgcgctcgcc gggggagcct ttgcccttga gccggattcg ctgaccgtcg gcaacgccgg
     2641 ccggaatgcg tacctgcatg gtgtgctcgc tcatcgcccg gccgctgccg tggcagacgg
     2701 gacaagggtc gtcgacgatg agaccgcggc cgcggcagtc gcggcacggc tctgagaacg
     2761 cgaacccgcc ggcacctcgc gaggtcacgc cggtgccact acacgtggga cacacgcgtg
     2821 gagtcgtgcc ggccttcgcg ccggtgccgt tgcaggcagt acacggatgc ggagtcgcca
     2881 tccgcaaggc gaccgtctcg ccgcgaaccg cgtgctcgaa gtcgagagtg acctcggttt
     2941 cgacgtcggc tccgcggcgc ggttgctgcg tcgtattggg acggcgacca ccaccgccga
     3001 agatgccgcc gaggatgtcg ccgatgccac cggctgcgcc ggcaccgccc gctcgtgcgt
     3061 tgccgagcag gtcaccgagg tcgaagttga agccgcccgg agccccgttg gcaccgccgg
     3121 aacttcccgg catccggaag ccgccgccgc cgaacagcga tcgcgcctcg tcgtactcct
     3181 tgcgcgacct ctgatcagac agcacgtcgt atgcctcgga gatctccttg aacttctcct
     3241 cagattttgc gccgccgcca gcgttcgcgt cggggtgatg gacgcgtgcg agtttgcggt
     3301 acgccttctt gatctcagcg gctgttgcat ctttggggac gccgagtgac ttgtagtagt
     3361 ccttctcgat gtagtcgcga gtgctcatcg gcgtccctcc cttctggctt gtttcttact
     3421 ggtcggacgg ttcttcgacg gtctcgtctg ccggcagcgc ggccgttggc tcggcgaccg
     3481 cgacccgtgc cggccgaatg acgcgacccg catagctgta gccggactgg aggatctgca
     3541 cggcggtcgg ctcgctgacc tcgtcggagt aggagtgcat cagcgcttcg tgctgggtgg
     3601 ggtcgaacgg gtcgcccggc tgaccgaaac gggtgagccc caacttggtg actgcagcat
     3661 cgagcgcctc ggcaacctga cggaatccgc cttcgagttc accgtgctcg cgagcgcggc
     3721 ctatgtcgtc gagcacgggc agtaatgccg ccaacgcctc ggcgaccgca aactcccgca
     3781 cggcttcgcg gtcacgctcg actcgcttgc ggtagttggc gtattcggcc tgtaggcgtt
     3841 gaagatctgc cgtgcgctcg gctacctgga tgcgcagctg tgagagttcg accgagtcga
     3901 cgccaccgcg agagccggga tctgaagcgc ccgccgaagc gggcccttcc cctgatcgcg
     3961 ggtcttgcga gaccgcaccg gacgccggat cgaaatgcac ccggcgcttg tcgcggacga
     4021 tcggctgatc gaacggatcc gacccgctca cgacttgccc tcgtcgacga tctcggcgtc
     4081 gatcacctcg tcagcgccgt cggtcgagcc ctcctcggag gcagagtgct cgtggtcggc
     4141 gtgaccggcg tcggcgctgt tggcgctgtt cgcctgtgca ttcgcgtaga tcgctgaacc
     4201 catcttctgg ctctccgcgg cgagctggtc ggtcgcggta cggatcgcgg cgaagtcgct
     4261 tccttcgagt gccttcttca agccggcgag cgctgtgtcg acggcgtccc tctcgggcgc
     4321 cggaaccgac tcggcgttgt cacgcaggaa cttctcggtc tggtacacga gggtctcggc
     4381 ctggttgcga gtctcggcgt cctccttgcg tgcacggtct tcctcggcgt gtgcctcggc
     4441 ttcgcgcatc atccgctcga tgtcgtcctt cggcagcgca gagccaccgg tgatcgtcat
     4501 cgactgctcc ttgccggtac cgaggtcctt ggccgacacg tgcacgatgc cgttggcgtc
     4561 gatgtcgaag gtgacctcga tttgcgggat tccgcgtggt gccggtggca gcccggtgag
     4621 ttcgaacatg ccgagctttt tgttgtacgc agccatttcg cgctcgcctt ggtacacctg
     4681 gatctgcacg ctcggctggt tgtcgtcagc cgtcgtgaac gtctccgacc gcttcgtcgg
     4741 gatcgtggtg ttgcgctcga tgagcttcgt gaacacgcca cccttggtct cgatgccgag
     4801 cgacaaaggc gtgacgtcga ggagcaaaac gtccttgacc tcacccttca tgacaccggc
     4861 ctgaaggctc gcgccgacag caacgacctc gtcggggttg acgcccttgt tcggctcctt
     4921 gccaccggtc agttcgcgga ccaggtcgac gacccctggc atccgagtcg aaccaccgac
     4981 caggacgacg tggtcgatct ggtcgacctt gatgccggcg tccttgatgg cctggtggaa
     5041 ggggcccttg gtgcgatcga gcaggtcaga cgtcatcttc tggaactcag acctggtgac
     5101 cttcacttcg aggtgcaggg gaccctcggc gctggccgtg atgtagggca ggttgaccgt
     5161 ggtctcggcc tgctgcgaca gctcgatctt ggccttctcg gctgcttcac gcagtcgctg
     5221 gagcgccatc ttgtctttcg ccaggtcgat gccattgctg ttcttgaacg ccgtcacgag
     5281 gaagtcgacg agacgctggt cccagtcgtc gccaccgagg tgggtgtcgc cgctggtcga
     5341 cttgacctcg acgatgccct cgccgatctc gagcagggac acgtcgaacg tgccgccacc
     5401 gaggtcgaag acgaggatcg tctgctcctt ctcacccttg tcgaggctgt aggccagagc
     5461 cgcagctgtg ggctcgttga tgatgcgcag gacgttcagg ccagcgatgg tgccggcctc
     5521 cttggtggcc tcgcgctggg agtcgttgaa gtaagcgggg acggtgatca ccgcgtcggt
     5581 gatctgctca cccaggtacg cctcggcgtc ccgcttaagc ttttgcagga tgcgtgcgct
     5641 gatctcctgc gaggtgtatt tcttgccgtc gatggtgacg gcccagtcgg tgccgatgtg
     5701 gcgcttgacc gaccggatgg tgcggtcggc gttggtgacg gcctgccgct tggcgacctc
     5761 accgaccagg acctcaccat tcttggcgaa cgcgacgacc gagggcgtag tgcgcgatcc
     5821 ttcggcgttc gcgatgaccg tgggctcgcc gccttcaaga acggcaacga cggagttcgt
     5881 cgtgcccagg tcgatgccga ccgctcgtgc catggggtgc tccttcaaag aactagggct
     5941 ctagctgaaa gttgagtctg atgcgctcat gttaatcggc gcacaagccg gcgtcaaaca
     6001 gacttgagcc ggtaaggctc aactcctgac aacctcgccg acaaccttgc gcttggccgc
     6061 gcccgggcgg tacgtgacgt taacgccaga cgtccagatg cgatcggacc gcgcaaatac
     6121 gggcgacccg tccagataca gaggggacgt ggcgcgatca ccctttcgcc ccgaaatgct
     6181 tcccgctaac ctcagggccc ggtaacggtt ggcacccccg acagaacggt tggtttacat
     6241 gtccttgacc cgcagattgc tccacgggcg tccgttccgc ttttttagcg gcggcgtggt
     6301 cgcgatcacg ttgatgggcg ggggctcgat cgtcaccgcc acggccgcga gcgccgtcac
     6361 gccggtgcca tcgaacgtcc accgcatcgc tggctcgacc cggctcgcga ctgcgatcgg
     6421 agcctcacag gaccagttcc cgaccgctgg cagcgcgagc gccgtcgtgt tagcccgctc
     6481 cgacacgttc ccggacgccc tagccggcgg gccgcttgca gcgaaagtgc acggcccatt
     6541 gctgctgacc tcgtcagcca gcctcgaccc tgcggtcagc gtcgaaatcc tgcgcgttct
     6601 gccggccgga ggcaccgttt acattcttgg cgggacggcg gcggtgagcg cagcggtcgc
     6661 cacgaccctc actgccaacc actacgtcgt gatccgcctc tccggcgccg accggtttgc
     6721 gaccgcggtc gccgtcgctg atcagatggg cgacccaacg acggtgttcg aggccaccgg
     6781 aacgaacttc cctgacgccc tggcaggcgg gcccgctgca atcaagacag gcggcgtgat
     6841 cctgctgacg aacggcgcca cccagtcgac tgcgacggcc gcctacctgg cggcacaccc
     6901 cggcggcact cactacgcgc ttggcggtcc ggccgccgca gccgatccgg tagcgacgcc
     6961 cctgatgggc gacgatcgct tctggacgtc catcgctgtc gctggcacct tcttcccgga
     7021 cgccacgact gtcggcgctg cgaccggatt gaacttcccc gacgcactgg cggccggttc
     7081 ggacctcgca gccaaggggg ctccgcttct cttggttccc actgcaggcg cgctgccgac
     7141 cgcgattgcg gtcgatctct tgaatcgctc cttcacggga tcgaacagcg tgctgctttt
     7201 cggtggcacg gcgtcggtaa gtgacgatgt tgcgacacaa ctcggcgtc
//
LOCUS       DASWVF010000594         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_322075, whole genome shotgun sequence.
ACCESSION   DASWVF010000594 DASWVF010000000
VERSION     DASWVF010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322075"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1816)
                     /locus_tag="VGD55_10430"
     CDS             complement(155..1816)
                     /locus_tag="VGD55_10430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0870803.1"
                     /translation="MAGTSGSAASAEFIDVLAAEAVPADFEPALMIPWQSGLDPVTGL
                     ADRRWFLRAVAMADAHPAGISIEVGIIAVRINGLRRIMHYYGRPAGDGVLLLAAEVLY
                     EWGRGDDLAARLGPDSFAILAHTGASGLTAAAARLRAKFAEVGVPATVGTAMRDAFGS
                     ASTALRAAKREIGKDTTALHAEDTASRAVAGARAATEAHVLQAQATGILMQWHHCGAE
                     RARLELAYQGHEMGRPVTAMARLLVAIAAGEPLAEEDAAHGIELQRALRLAANVAAES
                     GRRVPDVAVRKSVGAGRSTGTGQYTPGRQMQPVWSIRPPNAASGLLSLPVPDVVARAE
                     DLLLAGRYQGAANPSGSGGDWFDAFVLPDGTAALVLGDVAGHDALAAATMMQLRTLLR
                     GFAVSHEVAPSEVLRRLDRFFVQLDLGLLATAFFGWVHADPDGGLVLRWCNAGHLPPV
                     LLARDGETSVLECRDDLLLGLGVETARADLSLRLPDASTLLLYSDGLIETRTHDLDHG
                     LARLRAAARSLAALGIAELCDALVNAMANPTSHDDVTLLAVRVPC"
     gene            complement(1929..>2272)
                     /locus_tag="VGD55_10435"
     CDS             complement(1929..>2272)
                     /locus_tag="VGD55_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY0870804.1"
                     /translation="GLGALDLFRSVAGPMSTTQESNALLFADAAAQILLDLIDDANTN
                     NRGEPLDQWDQLASLSRIEVYQATGMLMAQLDLPPGAALSRLRAYAFVHELTAAEVAE
                     LVISRRLRMEV"
BASE COUNT          438 a          781 c          709 g          344 t
ORIGIN      
        1 tgacggcgga gggagtggaa acacagattc aacgtgacga agtccgcacg atcgggtgcg
       61 aagccgcgca gggcttcttc tacgcaaggc caatgcctgc ggcggccgtc agtgcgcatt
      121 tacagagcgt ttagcacccc cacgtaccgg acagctagca cggaactcgc accgcaagca
      181 acgtgacatc gtcgtgcgac gtcggattcg ccatcgcgtt gacgagtgcg tcgcacaact
      241 ccgcgattcc gagcgctgca agcgaccgag cagcggcgcg aagacgcgcc agcccgtggt
      301 caaggtcatg agtgcgagtc tcgatgagcc cgtcgctgta caagagcaac gtgcttgcat
      361 cgggcaatcg caacgacagg tcagctcgag cggtctcgac accgaggccg agtaggaggt
      421 cgtcgcggca ctcgagcacc gacgtctcac catctcgcgc gagcaggaca ggcggcaggt
      481 ggcctgcgtt gcaccagcgg agcacgaggc caccgtcggg gtcggcgtgt acccagccga
      541 aaaaggcggt cgcaagcagg ccgagatcca gttgcacgaa gaatcggtca agtcggcgaa
      601 gcacttcgct cggtgccacc tcgtggctga ctgcaaatcc gcgcaacagc gtgcgcaact
      661 gcatcatcgt cgcagccgca agcgcgtcgt ggccagcgac gtctcccaag accaacgcgg
      721 ccgtaccgtc gggcaggacg aaggcatcga accagtcgcc acccgaaccg ctcggatttg
      781 ccgcgccttg gtatcgaccc gcgagtaaca gatcctcagc tcgcgccacc acatccggca
      841 ctggcaatga cagcagaccg ctggcggcgt tgggcggtcg gatgctccac accggctgca
      901 tctgccgacc cggtgtgtac tgccccgtac ccgtagatcg gccggctccg accgacttgc
      961 gcaccgcgac gtccggcacc cgtcggccag actctgccgc gacgttcgca gccaaccgca
     1021 gggctcgttg aagctcgatg ccgtgcgccg catcctcctc ggcgagtggc tcgcccgcgg
     1081 cgattgcgac gagcaacctg gccatggccg tcaccggccg gcccatctca tgcccctgat
     1141 acgcaagttc gagacgtgcc cgctcagcgc cacaatggtg ccactgcatc aagattccgg
     1201 ttgcctgcgc ctggagcacg tgcgcctcgg tggccgcccg cgcaccggct accgcgcgcg
     1261 atgcggtgtc ctccgcgtgc aaggccgtgg tgtccttgcc gatctcacgc ttcgcggcgc
     1321 gcaacgccgt cgatgcgctt ccgaatgcgt cgcgcatggc cgtcccgact gtcgcgggca
     1381 cgccgacctc ggcgaacttc gcccgcaacc gcgcagcagc agcggtcaac ccggacgcac
     1441 cggtatgcgc caggatcgca aagctgtcgg gacccaaacg agccgccaga tcgtcgccgc
     1501 ggccccactc gtacagcacc tcagcggcaa ggagcagtac accgtcaccg gcaggccgcc
     1561 cgtagtagtg catgatgcgt cgcagcccgt tgatgcgtac cgcgatgatt ccgacctcga
     1621 tcgatatgcc cgcagggtgc gcgtccgcca ttgcgacggc tctgaggaac caacggcgat
     1681 cggccaagcc ggtcaccgga tcgagtccgc tctgccacgg aatcatcagc gcgggctcga
     1741 agtcggcggg cacggcctcg gcagcgagaa cgtcaatgaa ctcagcagac gcggcgctac
     1801 ccgatgtgcc agccaccacg atcaacctcc gcacaaacat gacgcgagcg gacgcgttgc
     1861 catggtctgg agcagcgcac tcgacgccag cggcgtcgag tcttgctaaa tgcacagctg
     1921 ggcctgtgtc acacctccat ccggaggcgt cgcgagataa cgagttcggc gacctcggct
     1981 gctgtcaact cgtgcacgaa cgcataggcc cgcaaccggc tcagcgccgc cccgggcggt
     2041 aaatcaagct gtgccatgag catgccggtc gcttggtaga cctcgattcg agaaagcgaa
     2101 gcgagctgat cccactggtc cagcggctca ccgcggttgt tcgtgtttgc atcatcgatc
     2161 aggtcgagca agatctgagc cgccgcatcg gcgaaaagca gagcgttact ctcctgagtg
     2221 gtcgacatcg gtccagcaac tgaccggaac aggtcgagcg ctccgagacc ga
//
LOCUS       DASWVF010000595         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_399002, whole genome shotgun sequence.
ACCESSION   DASWVF010000595 DASWVF010000000
VERSION     DASWVF010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399002"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VGD55_10440"
     CDS             complement(<1..1238)
                     /locus_tag="VGD55_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0870805.1"
                     /translation="MPDDATHAELVARHRYVMPSWMALYYDDPIEIVSGSGRRVTDGQ
                     GREYLDFFAGILTNMLGYNVPEVRAAIKAQADSGILHTSTLYLIRSQIELAEKIARLS
                     GIPDAKVFFTSSGTEANEAALLLATQYRRSNQVLALRNSYHGRSFASMAITGNRGWSA
                     SSLSPVNVSYVQSGYRYRSPFGHLSDADFIDACADDLRDTITTTTSGDVACMIAEPIQ
                     GVGGFATPPDGYFAAFKEILDSYGILFISDEVQTGWGRTGEHFWGIEAHGVVPDAMTF
                     AKGLGNGTSIGGVVARAEIMDCLNANSISTFGGNPLTTAAANATLDYVLSHDLQANAA
                     KVGTTLLEGIRGLQHAHPFIGDVRGKGLMIGVELVVPGSKTPDPAAATRMMEETRARG
                     LVIGKGGLHGNVLRVAPPLTL"
     gene            complement(1261..>2150)
                     /locus_tag="VGD55_10445"
     CDS             complement(1261..>2150)
                     /locus_tag="VGD55_10445"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0870806.1"
                     /translation="RPHADQPYALTGVKLGDEQFELSDKWDAAGRLALVGIGVEPGLS
                     DIFARYAADHLFSQVEEVGIRDGANLSVEGYDFAPTFSIWTTIEECLNPPVVWEKDRG
                     WFTTEPFSEPEVFDFPEGIGPVECVNVEHEEVLLVPRWVQAQRVTFKYGLGDEFIGVL
                     KTLHKIGLDRVDKIKVGGVEVSPRDVVAACLPDPATLGDRMRGKTCAGTYVTGLGKDG
                     QPRATYLYHVVDNEWSMREYGAQAVVWQTAVNPVVALELLATGAWKGAGVLGPEAFDA
                     QLFLDMLVAYGSPWGMRDQ"
BASE COUNT          380 a          692 c          707 g          371 t
ORIGIN      
        1 agcgtcagcg gaggcgcgac ccgcagcacg ttgccgtgca ggccaccctt accgatcacg
       61 agccctcgcg cacgcgtctc ttccatcatg cgcgtcgcag cggcagggtc aggggtcttc
      121 gaaccgggga cgacgagttc gacgccgatc atgaggccct tgccgcgcac gtccccgatg
      181 aaggggtggg cgtgctgaag accacgaatt ccttcaagca gagtcgttcc gaccttcgcc
      241 gcgtttgcct ggagatcgtg cgaaagcacg tagtcgaggg tggcattggc tgccgccgtg
      301 gtgagcggat tgccaccgaa cgtcgagatg gagttggcat tcaggcagtc catgatctcg
      361 gcgcgagcaa cgacgccacc gatcgacgtc ccgttgccaa ggccctttgc gaacgtcatc
      421 gcatcgggca cgacaccgtg ggcctcgatc ccccagaagt gttcgccggt acggccccag
      481 ccggtctgca cctcgtcgga gatgaacaag atgccgtagg agtcgaggat ctccttgaac
      541 gcggcgaagt agccgtcggg cggcgtggcg aacccgccca caccttgaat cggttcggcg
      601 atcatgcacg cgacgtcgcc cgatgtggtg gtcgtgatgg tgtcgcgcag gtcgtccgca
      661 catgcgtcga tgaaatctgc gtcggagagg tggccgaacg ggctgcgata gcggtagccg
      721 ctctgcacgt agctgacgtt gaccggcgac aggctcgatg cgctccagcc gcggttgccg
      781 gtgatcgcca tgctggcgaa tgagcgcccg tggtagctgt tgcgcaaagc cagcacctga
      841 ttgctgcgcc gatactgcgt agcgagcaag agcgcggctt cgttcgcctc ggtaccagac
      901 gaagtgaaga agaccttcgc gtcggggatt ccggagagcc gcgcgatctt ctccgcgagt
      961 tcgatctgcg atcggatcag atagagcgtc gaggtgtgca ggatgccact gtcggcctgc
     1021 gccttgatcg cggcgcgcac ctcggggacg ttgtagccga gcatgttggt gaggatcccg
     1081 gcgaagaagt cgaggtactc acgcccctgc ccgtcagtga cgcggcggcc ggagccagac
     1141 acgatctcga tgggatcgtc gtagtagagc gccatccacg acggcatcac atagcggtgg
     1201 cgcgccacca gttcggcgtg ggtcgcatcg tccggcatcg tcaaacactc cttcgaagag
     1261 tcactggtcg cgcattcccc atggtgagcc gtaggccacc agcatgtcga ggaagagttg
     1321 ggcatcgaac gcctcggggc cgagcacgcc agcgcccttc caggcgccgg tcgcgagcaa
     1381 ttcgagcgcg accacggggt tgaccgcggt ctgccagacc actgcctgcg ccccgtattc
     1441 gcgcatcgac cactcgttgt cgacgacgtg gtacaggtac gtggcgcgcg gctgaccgtc
     1501 cttgccgagc ccggtgacgt aggtgccggc gcaggtcttg ccgcgcatcc ggtcgccgag
     1561 cgtcgcgggg tcaggcaggc acgccgccac cacgtcgcgc ggggagacct cgacgccgcc
     1621 gaccttgatc ttgtcgacgc ggtcgagccc gatcttgtga agtgtcttca ggacgccgat
     1681 gaactcgtcc cccaacccat acttaaaggt gacgcgctgc gcctggaccc accgaggcac
     1741 cagcagcact tcctcgtgct cgacgttcac gcactcgacc ggaccgatcc cctcggggaa
     1801 gtcgaacacc tcgggctcac tgaacggctc ggtcgtgaac cagccacgat ccttctccca
     1861 gacaactggc gggttcagac attcctcgat ggtggtccag atagagaagg tcggcgcgaa
     1921 gtcgtacccc tctactgaga ggttcgcgcc gtcgcggatg ccgacttctt cgacctgcga
     1981 gaacaggtgg tcagccgcgt accgcgcgaa gatgtcggag agaccgggct cgacgccgat
     2041 accgacgaga gcgagccgcc ccgcggcgtc ccacttgtcg gaaagctcga attgctcgtc
     2101 accgagcttc acaccggtga gcgcgtacgg ctggtctgcg tgcggccgtg
//
LOCUS       DASWVF010000596        11548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_296752, whole genome shotgun sequence.
ACCESSION   DASWVF010000596 DASWVF010000000
VERSION     DASWVF010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296752"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VGD55_10450"
     CDS             <1..728
                     /locus_tag="VGD55_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY0870807.1"
                     /translation="ATALRGFEVGDLLIVSRTLRTAETLAARVDGRAMRMGHLEHELA
                     EADVVVTCTGADGVVLNVDVVERAMAARPETRPLFILDVAVPRDVDPRVAKLAGVTLA
                     DLDVLAPVVQHAAASDDVREARRIVDAELAAYVAARRASGVAPTVVALRDKAAQMVAT
                     ELHRLDRRLPDLDEAARQEIASTVRRVVDKVLHAPTVRVQELANTAGPESYPEALRRL
                     FDLDPAAPAAIASPDPTEPADQP"
     gene            725..1714
                     /gene="hemC"
                     /locus_tag="VGD55_10455"
     CDS             725..1714
                     /gene="hemC"
                     /locus_tag="VGD55_10455"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0870808.1"
                     /translation="MSPASTSMPTDTPTTVVRLGTRRSALAMAQATTVAQALRATGSA
                     VELVGVITDGDRSSTALTESGGVGVFVTQLREELLSGTVDVVVHSLKDLPTAPAPGLR
                     IAAIPTREDPRDALVARDRLTLATLPAGASVGTGSPRRAAQLRAIRSDLDIRPLRGNV
                     DTRIGKVAAGELDAIVVAAAGLARLGRLDEATQLLEVDVMAPAPGQGALAIECRDPDG
                     PGEPDDPRGEPARRFHDLLAIFDDPAARAAVTAERGLLAALEAGCTAPVGAFAEVGPA
                     LDLHLFAVVVSVDGVTAIRGSADGPASDAEGIGRRLAADLLARGAGTLMGTGL"
     gene            1761..3386
                     /locus_tag="VGD55_10460"
     CDS             1761..3386
                     /locus_tag="VGD55_10460"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0870809.1"
                     /translation="MRAAPKATSPKITGAVAFVGAGPGDPGLLTLRAVDLLGRADVVI
                     ADELPTSDLTRHCRADALIVDLSAVEADRPLTKAERAKVVVDAVKSGKSVVRLLAGDP
                     MFDGDGAEEAAACTKAKLNVEVVPGVPIAAAVPAYAGIALVDAKHREVRVIDARPSVS
                     GTSGPDWAAAAAGHQTIVVLRVEAPAAVAHELVQHGRSADTAVAATRAGTTTDQHTVV
                     STLGTLAADVKAAGLAGPVVLVVGDVVKSRSALSWFETRALFGWRVLVPRTKEQAGEL
                     SDRLRTYGAVPAEVPTIAVEPPRTPQQMERAVRGLVTGRYEWIAFTSVNAVRAVREKF
                     EEYGLDARAFSGIKVAAVGEQTAAALQAFGVKPDLVPAGEQSAAGLLEDFPPYDDILD
                     PINRVFLPRADIATETLVAGLVELGWEVDDVTAYRTVRAAPPPAATREAIKTGGFDAV
                     VFTSSSTVRNLVGIAGKPHATTVIACIGPQTAKTAEEHGLRVDVLAETPSVECLADAL
                     AAYGLAQRMVAEANGEWTGKPGRPRASSRRKAK"
     gene            3391..4443
                     /gene="hemB"
                     /locus_tag="VGD55_10465"
     CDS             3391..4443
                     /gene="hemB"
                     /locus_tag="VGD55_10465"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0870810.1"
                     /translation="MPHEIGSDAAELGRLVSRPRRLRRTPALRRLVAQTRLSASDLIL
                     PLFVKEGIREPAPIASMPGVVQHTRDSLRKAAAEAVAEGVGGLILFGIPANKDPRGSG
                     ADDPSGIVQLALRDLVAEVGDATVLMSDLCLDEYTDHGHCGIVVESPTGEQVIDNDAT
                     LLRYASIAVAQATAGSHVVAPSGMMDGQVAAIRTALDSAGFSEVAVLAYSAKYASALY
                     GPFRDAAECAPQFGDRRTYQQDSANSGEALREVALDIAEGADMVMVKPAMAYLDVVRR
                     VRDMVDVPVGAYQVSGEYAMVEAAAANGWIDRDAVILETLTSIRRAGADFVLTYWAAE
                     VARLLRDAPVSRRDQR"
     gene            4440..5138
                     /locus_tag="VGD55_10470"
     CDS             4440..5138
                     /locus_tag="VGD55_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0870811.1"
                     /translation="MNMTPTGTPPLAVLLDAGGVLLLPNPFTVAAVLRAAGGSLDPDV
                     VPRAHYAGVAAMDARKTADWSQYHRVFVRFADVPPDGVDDAMAGLASLFESPVPGTWN
                     VVPLGVADHLRDLAATGVAIGVVSNADGTVEATLRRCDIPFDVVIDSTLVGFAKPEPE
                     IFALALERLGVAASDAVHVGDMVSADVHGAHAAGVRPLHLDPFGDCPYPLGHHDHVQS
                     LGDVVALIAVPAPL"
     gene            5135..6451
                     /gene="hemL"
                     /locus_tag="VGD55_10475"
     CDS             5135..6451
                     /gene="hemL"
                     /locus_tag="VGD55_10475"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY0870812.1"
                     /translation="MNVGNATSQALFERAVAVTPGGVNSPVRAFRSVGGTPRFMASGH
                     GPYLVDVDGREYVDLVCSWGPLVLGHAYPAVVDAVTDAVAKGTSFGAPTRGEVELAEE
                     IVRRVRPVEQVRLVSSGTEATMTALRLARGFTGRSKVVKFAGCYHGHVDALLVSAGSG
                     LATLALPDSPGVTGTSAADTIVVPYNDLAAVEAVFEDYGDQIACIITEAAAANMGVVA
                     PLPGFNAGLLEIAHRHGALLILDEVMTGFRVSAAGWYGLDAVDADLFTFGKVMGGGLP
                     AAAFGGRADIMQRLAPVGPVYQAGTLSGNPLATAAGLATLRECTPKVYAHLDTTAADV
                     GRLVSDALSAAGVAHRLQFAGNLFSFFFTEHDVANFDDARAAESWRYSAFFHALLDNG
                     VYAPPSAFEAWFVSAAHDDVALERLADALPHGARAAATSRGPEVGA"
     gene            6448..7083
                     /locus_tag="VGD55_10480"
     CDS             6448..7083
                     /locus_tag="VGD55_10480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0870813.1"
                     /translation="MSERTVVHLLRHGEVANPDRILYGRLPGYHLSDAGVLMAKHAAQ
                     TVAGRDIVTLVSSPLDRAVETATPVAEQFGLEIRIDDRLIEASNVFEGLTFGVGDGSL
                     RRPAHWRYLINPFRPSWGEPYAELAARMLAAVASARDAARGHEAICVSHQLPIWTARR
                     KVERKRLWHDPRRRQCALGSLTSFTFDGDVIVNINYTEPSGHAGAGQVAGA"
     gene            7084..7701
                     /locus_tag="VGD55_10485"
     CDS             7084..7701
                     /locus_tag="VGD55_10485"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0870814.1"
                     /translation="MGRTSSTSRTSRLIAGALGAACALGLSACSASSAATSDQQTNYI
                     ALNGAGVVTTYAVGHRKVAPQVSGTDLDGKPLTLATYAGKVIVLNFWASWCPPCRSEA
                     PALEQVAVDTRASGVQFVGVDIRENGASDGVNFVNTHHIDYPSFADQSSRIALDFRST
                     GVQSPPTTIVIDRQGHIAARGLGEMTYSQLLSVVQKVAAEPEATT"
     gene            7698..8507
                     /locus_tag="VGD55_10490"
     CDS             7698..8507
                     /locus_tag="VGD55_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY0870815.1"
                     /translation="MIGLLAWHGSVGSSFQHTVTSGPLVLAIPVALLAGLVSFLSPCV
                     VPLVPGYLSYVTGLTGADIDDTRDGSGDGHRGHRRGRVLAGSVLFVLGFSAVFVTSGA
                     LFGYAGGNLRAHTVVVDRILGVVMIVLGLAFMGLIPGLQREARFHRLPAAGVAGAPVL
                     GFLFGVGWTPCIGPTLGAVQSLAFSGSSATQGALLTFAYCIGLGLPFIITALAFRHAV
                     GVLAVVKRHYALVTRIGAAMLIAVGVLLITGAWHTLVGDLQSVINSYVPAV"
     gene            8509..10188
                     /locus_tag="VGD55_10495"
     CDS             8509..10188
                     /locus_tag="VGD55_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY0870816.1"
                     /translation="MSETPTEVDDIATAIKLDTAPVDRDQGLALAETDELGTRQRAAR
                     TTATSVLGWLRWAWYSLTSMRTALILLFLLALAAVPGSVLPQHASNPLRVQEYYTKHP
                     TLAPIINRLGGFDVFGSPWFAAVYLLLFISLAGCVIPRSRRHLRAMRARPPAAPRNLA
                     RLPYFTSWTASGDVDTVVAASEKLLRAKRFRLDRAPTSVAAEKGHLRETGNLVFHLSL
                     LVVLVGVAVSSNFGFTGTVLVTEHSGFSDTAIAYDDLKTGPLVSRTKLPPFSFTLNSF
                     SATYQRTGSTRGAANSFDARVTWQPNPNAKPRPYDITVNHPLSSGGANVYLVGHGYAP
                     HFRVTDRTGQVFDQVTPFLPNGNTFESQGVVKLPDALPQQLGITGFFLPSAVEDPSGR
                     PISIFPAPDNPIAVIGVFAGDLGLDNGVPRSVYTLDTTHMTLLATTQLAPGQTLVLPN
                     GQGSVTFVGFDQWATFQISRDPGKGVVLVAVAFMVAGLLLSLRVRRRRFWVRAVPAGP
                     MSPGRTLVEVGGLTRTDASGGFDEEFAGLVAALKSELRPVPEDDVENLRED"
     gene            10191..11210
                     /gene="ccsB"
                     /locus_tag="VGD55_10500"
     CDS             10191..11210
                     /gene="ccsB"
                     /locus_tag="VGD55_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY0870817.1"
                     /translation="MGVDVGLATLSNELIAGGIAAYSVGFLAYAVDAAFGNHAPQLPG
                     EKVAPVDVRVPALVGARAGGAAADPSVVEPSQSDAGSGGSFFAAHIGRFAIAVTVIGW
                     LLQGGAIVSRGLSVHRLPWGNMYEFTLALTFTAVTAFLVIMMRQPARYLGVFVMTPVV
                     LALGIAVLVLYVAPAPLVPALHSYWLAIHVTAAIIASGVFTVATAATTMFLVADRYAT
                     AMAAGRPVRFSALARIMPSPDVLDRTAYRAYAFAFPVWTFAVIAGAIWAEYAWGHYWQ
                     WDPKETWAFITWVIYAGYLHARATAGWRGRKAAYIAMLGFTSILFNFFIVNIFISGMH
                     SYAGV"
     gene            11229..>11548
                     /locus_tag="VGD55_10505"
     CDS             11229..>11548
                     /locus_tag="VGD55_10505"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0870818.1"
                     /translation="MSFADLPDFLDRLEKDGDLVRVKAQVDPYLEITEIVDRVVRAGG
                     PALLFENVKGSKLPLAMNVFGTQARMAKALGVDNLDEIGARLGGLLKPELPKGFAGLK
                     DAMS"
BASE COUNT         1697 a         3833 c         3912 g         2106 t
ORIGIN      
        1 cggctacagc gctgcgcggg ttcgaggtgg gagatctgct catcgtgagc cgcacgctgc
       61 gaaccgcaga gacactcgct gcgcgtgtcg acggtcgcgc catgcggatg ggccatctcg
      121 aacacgaact cgccgaggcc gatgtggtgg tcacctgtac cggggccgac ggcgtggtgc
      181 tcaacgtcga cgtcgtcgaa cgcgcgatgg ccgctcggcc cgagacgcgg ccacttttca
      241 tcctcgacgt agccgtaccg cgcgatgtcg acccccgggt tgccaagctg gctggtgtca
      301 cccttgccga cctcgatgtg cttgctccgg tggtgcagca cgcggcggct tccgacgacg
      361 tgcgcgaggc gcgccgcatt gtcgacgccg agcttgccgc ttacgtcgcc gcgcgtcgtg
      421 cgtcaggggt tgccccgact gtggtcgcgc tacgcgataa ggccgcgcag atggtcgcga
      481 ccgaactgca tcggctcgat cgccgactgc cagatctcga cgaggcggcc cgccaggaga
      541 tcgcttcaac cgtgcggcgc gtcgtcgata aggtgttgca cgcgccgacg gttcgggtgc
      601 aggaactcgc gaacaccgcg gggccggagt cttacccaga ggcgttgcga cgattgttcg
      661 atctcgatcc ggcggcgcct gccgcgatcg cttcgccaga cccgaccgag cctgcggatc
      721 agccatgagc ccagcttcga cgtcgatgcc gaccgacaca ccgaccacgg tcgttcgcct
      781 tggcaccagg cgcagtgccc tggcgatggc gcaggcgaca acggtcgcgc aagcgctgcg
      841 tgccaccgga tccgcagtcg agctcgtggg cgtcatcacc gacggcgatc gcagcagcac
      901 tgcgctcact gagtcaggtg gcgtcggggt gttcgtcacg cagctccgcg aagaattgtt
      961 gagcggcact gtcgatgtcg tcgtgcattc gttgaaggat cttccgaccg cgcccgctcc
     1021 cggccttcgc atcgcggcga tcccgacccg tgaggatcca cgcgacgctc tcgtcgcgcg
     1081 tgatcgactg acgctggcga cattgcctgc gggcgcgagc gtcggcaccg gctcaccgcg
     1141 ccgcgcggca cagcttcgcg cgatccgctc tgacctcgac atcaggccgc tgcggggcaa
     1201 cgtcgacacc cgcatcggca aggttgctgc cggtgagctc gacgcgatcg tggtcgcggc
     1261 ggccgggctg gcccgcctag ggcggctcga cgaggcgacg caactgctcg aggtcgacgt
     1321 catggcgccc gcgccgggac agggagccct cgccatcgag tgccgcgacc ccgacggccc
     1381 cggcgagcct gacgaccccc gcggcgagcc cgcgcgacgg ttccacgatc tgttagcgat
     1441 cttcgacgat cccgccgccc gcgccgccgt caccgccgag cgcggccttc tcgcggcgct
     1501 cgaggcggga tgtacggccc ctgtcggggc atttgccgag gttggcccgg ctctcgattt
     1561 gcatcttttc gcggtggtcg tcagcgtgga cggcgtaacc gcgatccggg gctcggctga
     1621 cgggccggcg agcgacgccg aagggatcgg acgccgcctg gctgccgacc tgttggcgcg
     1681 aggcgccggc acgttgatgg gaactggtct gtgagatttt ccgttcgcac gtcacgcaca
     1741 cacgacagaa caggacgacg atgagggcag cgcccaaggc cacaagcccc aagatcacgg
     1801 gtgcggtggc gttcgttggt gccggaccag gcgaccccgg tctcttgaca ctacgggcgg
     1861 tcgacctgct cggtcgcgcc gacgtggtga tagccgacga gctgccgacc tccgacctca
     1921 cgcggcactg ccgtgccgac gctttgatcg tcgacctgtc ggcggtcgag gccgatcggc
     1981 cgctgacgaa ggcagagcgc gccaaggtcg tggttgatgc ggtcaagtcg ggcaagagcg
     2041 tggtcaggct gctcgcgggc gatccgatgt tcgatggtga cggcgccgaa gaggccgccg
     2101 cgtgcacgaa ggcgaaactc aacgtcgagg tcgtgccggg cgtcccgatc gcggccgctg
     2161 tcccggccta cgccggcatc gcactggtcg atgcgaagca ccgcgaagtg cgtgtcatcg
     2221 acgcccgtcc ctcggtgtca gggacgtccg gcccagattg ggctgctgct gccgcggggc
     2281 accagaccat cgtcgtgttg cgcgtcgagg caccggccgc cgtcgcgcat gagctcgtcc
     2341 agcacgggcg ctcggccgac accgccgtcg ccgcgacgcg cgccggtacg accaccgatc
     2401 aacacaccgt cgtgtcgacg cttggcacgc ttgcagccga cgtgaaggcc gcaggcctgg
     2461 ccggaccggt cgtgctcgtc gtcggcgatg tcgtgaagag ccgaagcgcg ctgtcgtggt
     2521 tcgagacccg cgcgttgttc gggtggcggg tgctggtgcc gcgcaccaag gagcaggccg
     2581 gcgagctgtc cgaccgcttg cgcacctatg gcgcggtgcc tgccgaggtg ccgacgatcg
     2641 cggtcgagcc gccgcgtact ccgcagcaga tggagcgggc agttcgcggt ctcgtgacag
     2701 gtcgctacga gtggatcgcg ttcacgtcgg tcaacgcggt tcgggcggtc agagagaagt
     2761 tcgaggagta cggcctcgac gcgcgcgctt tctcgggcat caaggtcgct gcggtgggcg
     2821 agcagaccgc agccgcgctc caggcgttcg gtgtgaagcc cgacctggtg ccggcaggcg
     2881 aacagtcggc ggccggcctg ctcgaggatt tcccgccgta tgacgacatt ctcgatccga
     2941 tcaaccgggt gttcctgccg cgcgccgaca tcgcgaccga gaccctggtg gctgggctcg
     3001 tcgaactcgg ctgggaagtc gacgacgtca ccgcctaccg cacggttcgg gccgcgcccc
     3061 cgccggcggc tacgcgtgaa gccatcaaga ccggcggctt cgacgcggtc gtgttcacgt
     3121 cgtcgtcgac cgtgcgcaat ctcgtcggca tcgcgggcaa gccgcacgcg acgaccgtga
     3181 tcgcgtgtat cgggccgcag acggctaaga cggctgaaga gcacggcctg cgcgttgacg
     3241 tgttggccga aactccgtcg gtcgagtgct tggccgatgc gctcgcggct tatggtctcg
     3301 cgcagcggat ggtggccgag gcgaacggcg agtggaccgg caagcctggt cggccgcggg
     3361 cgagttcgcg acgcaaggcg aagtagcgca gtgccgcacg agatcgggtc ggacgccgcg
     3421 gagctcggcc gccttgtgtc gcggccgcgc cggctgcgtc gtacacccgc attgcggcga
     3481 ctcgtcgcgc agacgcggct gtcggcgtcc gacctgatct tgcctttgtt cgtgaaggaa
     3541 ggcatccgtg agccggcgcc gatcgcgtcg atgccggggg tcgttcaaca cacgcgtgac
     3601 tcgttacgca aggcggctgc cgaagcggtt gccgagggtg tcgggggatt gatcctgttc
     3661 gggatcccgg caaacaagga tccgcgtggt tctggtgccg acgatccgtc gggcatcgtg
     3721 caactcgcgt tgcgcgatct ggtggccgag gtcggcgacg cgaccgtgct gatgagtgat
     3781 ctttgccttg acgagtacac cgaccacggc cattgcggaa tcgtggtcga gtcgccgact
     3841 ggtgagcagg tgatcgacaa cgatgcgacc ctgttgcgtt acgcatcgat cgcggtcgcg
     3901 caggccacgg cgggctcgca cgtcgtcgcc ccgagcggaa tgatggacgg ccaggtcgct
     3961 gccattcgca cagcccttga cagtgctggg ttttccgagg tcgcggtgct cgcttattcg
     4021 gccaagtacg cttccgcgct gtatgggcca tttcgcgacg ctgcggaatg cgcgccgcag
     4081 ttcggtgatc ggcgcaccta ccagcaagac agcgcgaata gcggcgaggc gttgcgcgag
     4141 gtcgcgctcg acattgcaga gggcgccgac atggtgatgg tgaagccggc gatggcctac
     4201 ctcgacgtcg tgcggcgggt gcgtgacatg gtcgacgttc cagtcggggc gtatcaagtc
     4261 agtggtgagt acgcgatggt cgaggcggcc gcggcgaacg gctggatcga ccgtgatgcc
     4321 gtgatcctcg agaccctgac gtcgatccgc cgcgcgggcg ccgacttcgt gctgacgtac
     4381 tgggcggccg aggtggctcg gttgctacgc gacgcgcccg tgtcgcgccg cgaccagcga
     4441 tgaacatgac accgaccgga acgccgccgc tcgccgtgct gctcgacgcg ggcggagtat
     4501 tactgctgcc caacccgttc accgttgccg ccgtgctgcg agccgctggt gggtcactcg
     4561 accccgacgt ggtcccgcgc gcgcactacg cgggtgtggc tgcgatggac gcgcgcaaga
     4621 ctgccgactg gagccagtac caccgggtct tcgtacggtt cgccgacgtg ccgcccgacg
     4681 gcgtcgatga cgcgatggcg gggctggcca gcctcttcga gtcgccggtg cccggcacct
     4741 ggaacgtcgt accactcggc gtcgccgacc acttgcgcga tctcgcagcc acgggcgtcg
     4801 ccattggtgt ggtgtcgaat gccgatggca ccgtcgaagc aaccctgcgg cgctgcgaca
     4861 tcccgttcga cgtcgtcatc gactcgaccc ttgtggggtt cgccaagccg gagccggaga
     4921 tcttcgcgct cgcacttgag cgactcggcg tcgccgcgag cgacgcagtg cacgtcggtg
     4981 acatggtgtc tgctgacgtc cacggggccc acgctgccgg cgtccggccg ttacatctcg
     5041 acccgttcgg agattgtcct tatcccctag ggcatcacga ccacgtgcag tcgcttggcg
     5101 atgtcgtcgc cctcatcgcc gtaccggcgc cgctgtgaac gtcggcaacg cgacgtcgca
     5161 agccctcttc gagcgcgctg tcgcggtgac gccgggggga gtgaactcac ccgtccgagc
     5221 atttcgcagc gtcggtggca ccccgcgttt catggcgagc ggccacgggc cctacctcgt
     5281 cgacgttgac gggcgtgagt atgtcgatct cgtctgctcg tggggtccac tggtgctcgg
     5341 ccacgcctac ccggcggtcg ttgacgccgt caccgatgcc gtggccaaag gcacgagctt
     5401 cggcgcaccg acgcggggcg aggtcgaact cgccgaggag atcgtccgcc gggtccggcc
     5461 cgtcgagcag gtgcggttgg tgagctcggg taccgaggcc acgatgacgg cgcttcggtt
     5521 ggcacgcggc ttcaccggcc ggtcgaaggt cgtgaagttc gcgggttgct accacggcca
     5581 cgtcgacgct ctgctcgtat cggcgggatc cggtcttgcc acgttggcac tccccgattc
     5641 gccgggagtc acaggtacct cggccgccga cacgatcgtg gtgccctaca acgatctcgc
     5701 agcagtcgaa gcggtgttcg aggactatgg cgatcagatc gcgtgcatca tcaccgaggc
     5761 ggctgccgcg aatatgggtg tcgtcgcgcc acttcccggt ttcaacgctg gcctgctcga
     5821 gattgcccat cgccatggcg cgctattgat tctcgacgag gtcatgaccg gattccgcgt
     5881 ctcagcggcg ggctggtacg gcctcgatgc agtcgatgcc gacctgttca cgttcggcaa
     5941 ggtgatgggc ggtgggctgc cggcggctgc gttcggaggt cgcgccgaca tcatgcaaag
     6001 gctcgctcca gttgggcccg tctatcaagc gggcacgttg tcgggtaacc ctcttgcgac
     6061 tgctgcaggg ctcgcgacac ttcgcgagtg cacgccgaag gtctatgcgc acctcgatac
     6121 aacggcggcg gacgtcggcc ggcttgtctc tgatgcgttg agtgcggctg gagttgcgca
     6181 tcggctccag ttcgcgggga acctgttctc gttcttcttc accgagcacg acgttgccaa
     6241 cttcgacgat gcgcgtgccg cggagtcgtg gcgttactca gccttctttc acgcgctgct
     6301 cgacaacggt gtttacgcac cgccgtcggc cttcgaagct tggttcgtct cggctgcaca
     6361 cgacgacgtg gcgttagagc gcctcgctga cgctttgcct catggtgccc gcgcagctgc
     6421 aaccagccgc gggccggagg tcggcgcatg agcgagcgca ccgtcgttca tttgcttcgc
     6481 cacggcgagg tagcgaaccc cgaccggatt ctctacggca ggctgcctgg gtatcacctt
     6541 tccgatgctg gcgtgctgat ggccaagcat gcggcgcaga cggtcgcggg ccgtgacatc
     6601 gtcacgctgg tgtcgtcacc gcttgaccgt gcggtggaga cggcaactcc tgtcgcggag
     6661 cagttcgggc tcgagattcg tatcgacgac cggttaatcg aagcctcgaa cgtgtttgaa
     6721 ggcctgacct tcggggtcgg tgacgggtct cttcggcggc ccgcgcattg gcgctatctg
     6781 atcaatccgt tccggccttc gtggggcgag ccgtacgccg agttggcggc gcgcatgctc
     6841 gccgcggtgg ccagtgcgcg tgacgccgct cgtggacacg aggcgatctg cgtcagccac
     6901 caactgccga tctggacagc tcgccgaaag gtcgaacgca agcggctctg gcacgaccca
     6961 cgtcgccggc agtgcgcgct tggcagtctc accagcttca cgttcgacgg cgacgtcatc
     7021 gtgaacatca actacaccga accttccggc cacgcaggcg ccgggcaggt cgcgggcgcc
     7081 tagatgggcc gcacatccag cacctcccgc acctcccgcc ttatcgccgg ggcgctcggc
     7141 gctgcgtgcg cgctcgggct tagtgcgtgc tccgcatcga gtgctgccac cagtgaccag
     7201 caaaccaact acatcgcgct gaacggcgcc ggcgtcgtga cgacgtatgc agtcgggcat
     7261 cgcaaagtcg ccccgcaggt ttcgggcacc gatctcgacg ggaaaccgct caccttggcc
     7321 acctacgccg gcaaggtcat cgtcttgaac ttctgggcgt cgtggtgccc gccgtgccgc
     7381 agcgaggcgc cggctcttga gcaagtggcc gtcgataccc gcgcgtctgg cgtgcaattc
     7441 gtgggcgtcg acatcaggga gaacggcgcg tccgatggcg tgaacttcgt caacactcac
     7501 cacatcgact accccagctt cgccgatcag tcgtcgcgca ttgcgctcga cttccgcagc
     7561 accggagtgc agtcgcctcc gaccacgatt gtcatcgacc ggcaagggca catcgccgca
     7621 cgcggccttg gcgagatgac gtactcgcaa ctgctgtcgg tcgtgcaaaa ggtcgccgcc
     7681 gaacctgagg cgacgacatg atcggtctgc ttgcgtggca cggctccgtc gggagttcct
     7741 tccagcacac ggtcaccagc ggcccacttg tcttggcgat accagtagca ctcctggccg
     7801 gcctcgtatc gttcctttcg ccctgcgtgg tgccgcttgt gccgggctac ttgtcttacg
     7861 tcacaggcct tacaggcgcc gacatcgacg acacgcgcga cggctctggt gatgggcacc
     7921 gcggtcatcg tcggggccgg gtgctcgcgg gcagcgtgtt gttcgtcctc gggttctcgg
     7981 cggtgttcgt gacgtcagga gcgctcttcg gctatgcggg cggcaacttg cgcgcacaca
     8041 cggtggtcgt cgaccggatt ctcggcgtgg tgatgatcgt gcttggtctc gcgttcatgg
     8101 gcctgatccc cggactgcaa cgcgaagcgc gcttccaccg gctgccagca gcgggagtcg
     8161 cgggtgctcc tgtgctcgga tttcttttcg gtgtcgggtg gacgccgtgc atcgggccga
     8221 cactgggcgc cgtccagagc ctcgcatttt cagggtcaag cgcgacgcag ggtgcgctgc
     8281 tgacgttcgc gtactgcatc ggcctcggct tgccgttcat cattactgcg cttgcgttca
     8341 ggcacgccgt cggcgtgctc gcggtcgtca agcggcatta cgcgctggtc acccgcatcg
     8401 gcgcggcaat gctcatcgcg gtaggtgtac tgctgatcac cggtgcgtgg cacaccctcg
     8461 tcggcgacct ccaatcggtc atcaacagct acgtgccggc ggtctgagat gagcgagact
     8521 ccgaccgagg tcgacgacat tgccaccgcc atcaaactcg acaccgctcc ggtcgatcgc
     8581 gaccaagggt tagcgctcgc cgaaaccgac gagctgggca ctcgccagcg tgccgcccgc
     8641 acgaccgcga catcggtctt ggggtggctg cggtgggcct ggtattcgct gacgtcgatg
     8701 cgcaccgcgc tgatcctgct gttccttctt gcgctcgccg cggtcccggg aagcgtgctg
     8761 ccacaacacg cgagcaaccc gttgcgggtg caggagtact acaccaagca cccgacgctc
     8821 gcgccgatca tcaacaggct cggtggcttc gacgtgttcg gttcgccgtg gttcgcggcg
     8881 gtctacctgc tgctgttcat ctcactcgca ggttgcgtga ttccccgcag ccgtcgccac
     8941 ctgcgagcga tgcgtgcccg accacctgcg gctccgcgca atctcgcccg cttgccatat
     9001 ttcacatcgt ggacggcgag tggtgacgtc gacacggtcg ttgccgcatc ggagaagttg
     9061 ctgcgcgcca agcggtttcg cctcgatcgc gcgcccacaa gcgtcgccgc agaaaagggc
     9121 cacctgcgcg agaccggaaa cctggtgttc catctgtcgt tgcttgtggt gctggtcggc
     9181 gttgcggtct cgtcgaactt cggcttcacc ggcacggtgc tggtgaccga acattcgggg
     9241 ttttccgaca cggcgatcgc gtacgacgac ctcaagaccg gtccgctcgt cagccgcacc
     9301 aagctgccgc cgttctcctt cacgttgaac tcgttcagcg cgacgtacca gcgcaccggc
     9361 tcgactcgtg gggccgcgaa cagcttcgac gcgcgggtga cctggcagcc gaacccaaac
     9421 gccaagccgc gcccttacga catcaccgtc aaccatccgt tgagctcggg cggcgcgaac
     9481 gtctacctgg tcggccacgg gtacgcgccg cattttcgcg tcaccgaccg caccggccag
     9541 gtgttcgatc aggtgacgcc gttcttgccc aacggcaaca cgttcgaatc gcaaggtgtc
     9601 gtcaagttgc ctgacgcgtt gccgcagcaa ctcggcatca ccggcttctt cctgccttcg
     9661 gcggtcgaag acccgagcgg ccggccgatc tcgatcttcc ccgcacccga caacccgatc
     9721 gcggtcatcg gggtgttcgc gggtgacctc ggtctcgaca acggggtgcc gcgctcggtc
     9781 tacacgctcg acacgacgca catgaccctc ctcgcgacga cgcagttggc gcctgggcag
     9841 acgttggtgt tgccgaacgg gcagggcagc gtgacgttcg tgggcttcga tcagtgggcg
     9901 actttccaga tcagccgcga ccccggcaag ggcgtcgtgc tcgtggcggt cgctttcatg
     9961 gtcgccggat tgttgctctc tctgcgggtg cgcaggcgcc ggttctgggt gcgggcggtg
    10021 cctgcaggtc cgatgtcgcc tgggcgtacc ttggtcgagg tcggtgggct cacccgcacc
    10081 gatgcaagtg gcggcttcga cgaggaattc gccggcctgg tggcggctct caagtcggag
    10141 cttcggccgg tgccagaaga cgacgtcgag aaccttcggg aggactgatc gtgggcgtcg
    10201 atgtcggcct ggccacgctg tcgaacgagc tcatcgccgg tggcattgcg gcgtactcgg
    10261 tcgggttcct tgcttacgcg gtagacgctg ctttcggcaa ccacgcgccg cagttgcctg
    10321 gcgagaaggt ggcaccagtc gacgtccgcg tgcctgctct tgtcggtgca cgtgctggcg
    10381 gcgctgcagc cgacccttcg gtggttgagc cgtcgcagtc ggacgccggt tctggcggtt
    10441 cgttcttcgc cgcgcacatc ggccggttcg ccatcgccgt caccgtcatc gggtggttgc
    10501 ttcagggcgg cgcgatcgtc agccgcggct tgtccgtgca ccggttgccg tggggcaaca
    10561 tgtacgagtt cacgttggcg ctgacattca cggcagtcac ggcatttttg gtgatcatga
    10621 tgcggcagcc ggcgcggtat ctcggcgtct tcgtcatgac gcccgtggtg ctggcgctgg
    10681 gtattgcagt gctggtgttg tacgtcgcgc ccgcgccact ggtgccggcc ctgcactcct
    10741 actggctggc gatccacgtg acagccgcca tcatcgcgtc aggggtgttc accgttgcga
    10801 ccgccgccac gacgatgttc ctcgtcgccg accggtacgc caccgcgatg gcagcaggtc
    10861 ggccggtcag gttttcggcg ctggccagga tcatgccctc gcccgacgtc ctcgaccgca
    10921 cggcctatcg ggcgtacgcg ttcgcgttcc cggtctggac cttcgcggtc atcgccggcg
    10981 cgatctgggc cgagtacgcg tggggccact actggcagtg ggacccgaaa gagacctggg
    11041 ccttcatcac ctgggtgatc tacgccggct acctgcacgc cagggccacg gccggctggc
    11101 gcggacgcaa ggccgcctac atcgcgatgc tcgggttcac ctcgatcctg ttcaacttct
    11161 tcatcgtgaa catcttcatc agcggcatgc attcgtacgc cggggtgtag acccgcgata
    11221 cttgatctat gagcttcgcc gacctgcccg acttcctcga tcgcctggag aaggacggcg
    11281 acctcgtgcg ggtcaaggcg caggtcgacc cttaccttga gatcaccgag atcgttgacc
    11341 gggtcgtgcg ggcaggcggc cctgcgctgc tgttcgagaa cgtcaagggc tccaagctcc
    11401 cgctggccat gaacgtgttc gggacccagg cccggatggc gaaggcgctc ggcgttgaca
    11461 acctcgacga gatcggcgcc cgcctcggtg gtctgctcaa gcctgagctc ccgaaaggct
    11521 tcgccggcct caaggatgcg atgagcaa
//
LOCUS       DASWVF010000597         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_220931, whole genome shotgun sequence.
ACCESSION   DASWVF010000597 DASWVF010000000
VERSION     DASWVF010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220931"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGD55_10510"
     CDS             complement(<1..295)
                     /locus_tag="VGD55_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0870819.1"
                     /translation="MFTGIIEELGTIVDVRETAVGSERAVRMTVGGPLVCADVHAGDS
                     IAVSGVCLTVVDSAAGQFSADVMLETLTRSTLARARNGVAVNLERAVTLQTRLG"
     gene            complement(298..1329)
                     /gene="ribD"
                     /locus_tag="VGD55_10515"
     CDS             complement(298..1329)
                     /gene="ribD"
                     /locus_tag="VGD55_10515"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0870820.1"
                     /translation="MSAPESDASLMRRAIELAELGLGSTSPNPIVGAVIVDGDGTVVG
                     QGFHARAGEPHAEVVALRDAGGRAQGATVFVTLEPCRHTGRTPPCTQALIDAGVSRVV
                     YAVDDPDPVAGHGAANLRTAGIAVEAGLLHDEAARSNAAWLHFTRTGRPYVTWKYAAT
                     LDGRVSAADGSSRWITGEAARHDVHVLRSRSDAIVVGTGTVLADDPRLDVRLPGGAIT
                     HQPLRVVVGRREIRHTAKVFGGSAPSLQIADHDLASVLKSLAERDVVSVLIEGGPTLA
                     GAFVAAGLVDRIVAYIAPALLGSGLPALGDAGIETLSDALRFHVEAIDLVGDDVRITA
                     TTSAPKRSE"
     regulatory      complement(1425..1574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1593..>2070)
                     /locus_tag="VGD55_10520"
     CDS             complement(1593..>2070)
                     /locus_tag="VGD55_10520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0870821.1"
                     /translation="SGGPCAVAYLDLDNFKAFNDAHGFLRGDKVILLLATALRRAIVG
                     VVPPAFIGHVGGDDFVLVCRPDQVEDLSRAAVGYFDDHVPALHDADEVARGYLEMADR
                     LGVLRQYPLVSVSVGIATSERRTFSDYREVVVVATEMKGVAKGASGSAIAVDRRAV"
BASE COUNT          367 a          725 c          660 g          318 t
ORIGIN      
        1 cgccgagccg cgtctgcaaa gtgacggccc gctcgagatt gacggcgaca ccgttacggg
       61 ccctcgcaag ggtcgaccga gtcagtgtct caagcatgac gtcggcgctg aactggccag
      121 ccgccgagtc gacaaccgtc aaacacacac ccgacaccgc aatcgagtca ccggcgtgaa
      181 catcggcgca taccagcggc ccgccgaccg tcatccgaac ggcccgctcg ctgccgaccg
      241 cggtttcgcg cacatcgact atcgtgccga gctcttcgat gatgccggtg aacacgctta
      301 ctccgatcgc tttggtgctg aagtcgtcgc cgtgatccgg acgtcgtcgc ccacaaggtc
      361 aatcgcctcg acgtgaaatc tcaaggcgtc ggacagggtt tcgattcccg cgtcgccaag
      421 agccggcaga cccgaaccca gcagcgcggg cgcgatgtag gcgacgatcc ggtcgacaag
      481 gccggcggcg acgaaagccc ccgcgagagt tgggccgcct tcgataagca cgctgacgac
      541 gtctcgctcc gcgagggact tcaggaccga cgccagatcg tggtcggcga tctgcagact
      601 tggagccgag ccgccgaaga cctttgccgt atgccggatt tcacggcgtc cgacgacaac
      661 ccgcaacggc tggtgggtga tggccccgcc cggtagccgg acatcgagtc gcggatcatc
      721 ggccagcaca gtgcctgttc cgacgacgat ggcgtcactg cgagaccgca gcacatgcac
      781 atcgtgcctg gcggcttcgc cggtgatcca ccggctcgag ccgtcggctg cactcacccg
      841 accgtcgagt gtcgctgcat atttccaggt cacgtacggg cggccggtgc gggtgaagtg
      901 cagccatgcg gcattagaac gggcggcctc gtcgtgcagc agacctgcct cgacagcaat
      961 gcccgcggtt cgcaggttgg cagcaccgtg acccgcgacg gggtcggggt catcgacggc
     1021 gtacacgaca cgggagaccc ccgcgtcgat gagagcctgc gtgcacggcg gcgtgcggcc
     1081 ggtgtgtcga cacggttcga gggtgacgaa aacggttgcg ccctgcgcgc gccccccggc
     1141 gtcccgtaaa gcaacgactt cagcgtgcgg ctcaccggct cgcgcgtgga agccctgacc
     1201 gacaaccgtg ccgtctccat cgacgatcac cgcgccgacg atcgggttgg gactggtcga
     1261 gccgagaccg agttctgcca gctcgatggc gcgacgcatc aagctggcgt cggactctgg
     1321 cgcgctcatc tgtctcctcg cctcgaccgg ccgcaacgca cgcggacaag gcgacgaaga
     1381 cgtcatgcga acgtgcccgc gcacgcttgg cgcacggaca gctcccgcgc agcctcccat
     1441 ccggactttc accgtcggtc ccggaattcc accgagtcaa ccgtccgagg gagacgcagt
     1501 ttcctgcgta gccttcggcc gggtcgcgga ctgtcaccgc cggttcggaa ttacaccgac
     1561 cccggagcgc acgtgcttgt acccctctag tttcacacag cgcggcggtc gacggcgatc
     1621 gcggagccgc tcgcgccctt cgcgacgcct ttcatctcgg tcgcgaccac cacgacctcg
     1681 cggtagtcgc tgaacgtgcg gcgctcgctc gtggcgatgc cgaccgagac cgagaccaac
     1741 ggatactggc gcagaacgcc caaccggtcg gccatctcga ggtacccgcg cgccacctcg
     1801 tcggcatcgt gcagcgccgg cacgtggtcg tcgaagtatc cgactgccgc gcggctcagg
     1861 tcttcaacct ggtcagggcg gcagaccaga acgaagtcgt cacctccgac gtgcccgatg
     1921 aacgccggcg gcaccacacc gacgatcgct cggcgcaacg ctgtggcgag caacaggatg
     1981 accttgtcgc cgcgcaagaa tccgtgcgcg tcgttgaacg ccttgaagtt gtcgaggtcg
     2041 aggtacgcca cagcgcacgg cccgccactg
//
LOCUS       DASWVF010000598         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_169171, whole genome shotgun sequence.
ACCESSION   DASWVF010000598 DASWVF010000000
VERSION     DASWVF010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169171"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VGD55_10525"
     CDS             <1..855
                     /locus_tag="VGD55_10525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0870822.1"
                     /translation="KAYVQPDGSIKTFRPDANARRFQRSAQRLALPELPVDDFVAAAD
                     LLIEADRAWVPAEDEKSLYIRPFMMASEAFLGVRPANHVTFLVIASPAGAYFAGGLHP
                     VSIWLSEEYTRAAPGGTGAAKCAGNYAASLIAQQEASANGCDQVVFVDALEKRWVEEL
                     GGMNVFFVFDDGSLVTPPVSGTILEGVTRDAIRTIAAEHGHATVERPIDIDEWREGVT
                     SGRITEVFACGTAAVVTPLGRLAWRGGELTMGDGEPGKVTQDIRDSLLALQYGHAPDT
                     HGWMHTVC"
     gene            863..1399
                     /locus_tag="VGD55_10530"
     CDS             863..1399
                     /locus_tag="VGD55_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870823.1"
                     /translation="MEPNAGVGCLDGMYSLTQDAGAPRLAQDLPAQAGFEEVYRRCRP
                     GLVRLGHLLTGSAAAAEDVVHDAFVGYARAGSKVTDPAAYLRRSVVNLSMNVHRRSTR
                     ERRHLGTLTESVVEPTEVDETWLLVRQLPPRQRAVLVLRFYLDLSEAETARVLGCRPG
                     TVKSTTSRALAKLKETMS"
     gene            1396..2373
                     /locus_tag="VGD55_10535"
     CDS             1396..2373
                     /locus_tag="VGD55_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870824.1"
                     /translation="MTPNDDEVLARLRAALRTVAERTPISGGRLEDVWAAPAQKHSRP
                     MVTIVSAAAAVAAIAAVTAVLADTHSAGSQSLVPASAGESSESSLSPTPTVTVAPSPV
                     PSPTPTITATPSPLLAAADCVPENYYVVASPSEVAGLTYMLPATPAGYVLYGAWGTIS
                     RNQCANSVTWYAEYDPVSGAPGDGTNAIQVTVTKLGGDSLEGDFASARPVASTPITVN
                     GIEGYVFSKDNAYTAIGWTSGGAQITLAGPISHGDIQSLVTVADSLTLLSPNDPRIVA
                     PADCQVPAGSVCGSDSATPSPSLSPSTDSRTATVAPTPYPTTPTPTPTP"
BASE COUNT          439 a          987 c          938 g          440 t
ORIGIN      
        1 aaggcctacg tgcagccgga cggctcgatc aagacgtttc ggccggacgc gaatgcgcgc
       61 aggttccagc ggtcggcgca gcgactcgcg ctgccggagc taccggtcga cgacttcgtc
      121 gcggctgccg acctgctgat cgaggccgat cgcgcgtggg tgccggctga ggacgagaaa
      181 agcttgtaca tcaggccttt catgatggcg agcgaggctt tcctcggcgt ccggcctgca
      241 aatcacgtga cgttccttgt catcgcgtcg cccgccggcg cgtatttcgc tggtgggctg
      301 cacccggtct cgatctggct gtcggaggaa tacacccgcg cggctccagg cggtaccggc
      361 gcggcgaagt gcgcggggaa ctacgcggcc agcctgatcg cgcagcaaga ggcgagcgcg
      421 aacggctgcg accaagtggt gttcgtcgat gcgctcgaga agcgttgggt cgaagagctc
      481 gggggcatga acgtcttctt cgtgttcgac gacgggtctt tggtgacgcc gccggtgtca
      541 ggaacgatcc tcgagggtgt gacgcgtgac gcgatccgca cgatcgccgc tgagcacggc
      601 cacgcgacgg tcgagcggcc aatcgacatc gacgagtggc gcgagggcgt gacgtcgggc
      661 cgcatcaccg aggtgttcgc ctgcggaacg gccgctgttg tcacgccgtt gggccgcctg
      721 gcctggcgcg ggggagagct cacgatgggc gatggcgagc ccggcaaggt gacccaggac
      781 atccgcgact ccctgctcgc actccagtac ggccacgcgc ccgacaccca cggctggatg
      841 cacaccgtct gctaacgctc cggtggaacc gaatgccggg gtggggtgtc ttgatggcat
      901 gtacagcctg acgcaggacg ccggtgcgcc gcggctcgcc caggacctcc cggcccaagc
      961 ggggttcgaa gaggtctacc ggcggtgccg cccggggctg gtacgcctcg ggcacctgct
     1021 gaccggatcg gccgcagcgg cggaggacgt cgtgcacgac gcgttcgtcg gctatgcccg
     1081 cgccggatcg aaggtgaccg atcccgctgc ctacctgcgg cggtcggtcg tgaacctgag
     1141 catgaatgtc caccgccgca gcacccgcga acgccgccac ctcggcaccc tcaccgagtc
     1201 ggtcgtcgaa ccgaccgagg tcgacgagac gtggctgctc gtcaggcagt tgcccccgcg
     1261 ccagcgcgcc gtgctggtgc tgcggttcta cctcgatctg tccgaagccg agaccgcccg
     1321 cgtcctcgga tgccgtccgg gcacggtcaa atcgacgacc tcgcgcgcgc tcgccaagct
     1381 caaggagacg atgtcatgac acccaacgac gacgaagtgc tggcgcgcct gcgggctgct
     1441 cttcgaacag ttgcagagcg gacgccgatc agcggcggcc gtctcgaaga tgtgtgggcc
     1501 gccccggccc agaagcactc gcgcccgatg gtgacgatcg tgtcagcggc cgcagcggtc
     1561 gccgcgatag cggcggtgac cgcagtgctt gcggacaccc attcggccgg ttcgcagagt
     1621 ttggtgcctg cgtcggcggg tgagtcgtct gagtcgtctt tgtcgccgac gccgacggtc
     1681 acggtggccc cgagcccggt acccagcccg accccgacga tcacggcaac cccgtcgccg
     1741 ttgttggcgg cggccgactg cgtgccggag aactactacg tggtcgcctc gccgtccgag
     1801 gtcgcaggat tgacgtacat gctgcccgcg acacccgcag gctacgtgct ctatggcgcg
     1861 tggggcacca tctctcgcaa ccagtgcgcg aacagcgtca cgtggtacgc cgagtacgac
     1921 cccgtctccg gcgcgcccgg cgacggcacg aatgcgatcc aggtcaccgt gaccaaactc
     1981 ggcggcgatt cgcttgaggg cgacttcgcc agcgcgcgac ctgtcgcctc gaccccgatc
     2041 acggtcaacg gcatcgaggg gtacgtgttc agcaaggaca atgcgtacac cgccatcggc
     2101 tggaccagcg ggggagcaca gatcaccctc gccggtccga tcagccacgg cgacatccag
     2161 tcgctcgtca ccgtcgccga ctcgttgacg ctgctgagcc cgaacgaccc gcgcatcgtt
     2221 gcaccggccg attgccaggt gccggcaggc agcgtgtgcg ggagcgacag cgcgacaccg
     2281 tcgcccagcc tctcgccgtc caccgattcg cgtaccgcaa ccgtcgcgcc gaccccttac
     2341 ccgacaaccc cgaccccgac gcccaccccc tgacgcccac ccccgggccc gtttcttgaa
     2401 cttggaaggg cgtggacgcc caagagcgcg tccctgcctt tccaagttcg gcggtggcgg
     2461 cgttgtttgg tcgatcgggg gcgtcactag gcgtgaccag gtcgtacatc gtgtggcaca
     2521 ctgaccgggt gacgtcttcg cagctgatta tcgcctaggc gcgccggtcc agatccttag
     2581 accagcgcgc ccgacctccc gcatctgcgg ggggtctttt tgttgtgtcc gacagtgtcc
     2641 gacccgcccg cgcccgaccc gcccgcgccc aacccgcccg cgcccacccg gagaggtacc
     2701 ccgatgtccc cgcagcccga cgcctttcac gtctacgaca cgaccctgcg cgacggcgcc
     2761 cagggtgagg gcatggccct cacggtcgcc gacaagctgg ccat
//
LOCUS       DASWVF010000599         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_347969, whole genome shotgun sequence.
ACCESSION   DASWVF010000599 DASWVF010000000
VERSION     DASWVF010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347969"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VGD55_10540"
     CDS             <1..420
                     /locus_tag="VGD55_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0870825.1"
                     /translation="RELVDLLESRGVEVFYAIHPVAGRMPGHMNVLLAEANVPYEQLK
                     EMDDANPEFSSTDVAVVVGANDVVNPAARDAPGSPIYGMPILNVDEATNVVFMKRSMR
                     PGFAGIDNALLYNAKTTLFFGDAKDSLSKLVAAVKAV"
     gene            complement(417..1064)
                     /gene="upp"
                     /locus_tag="VGD55_10545"
     CDS             complement(417..1064)
                     /gene="upp"
                     /locus_tag="VGD55_10545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY0870826.1"
                     /translation="MRTLVADHPLIAHKLTALRDQRTDSPTFRRLVDELVTLLAYEAT
                     RDVRTTDVDVVTPVAHARGTRLARPRPLVVPVLRAGLGMLEGMCRLLPTAEVGFLGLV
                     RDRETLVATTYASRLPDSLAGRQVYVLDPMLATGGTLSAAIDLLVERNADQVTAICLL
                     AAPEGLAFMESHFADSKVPLSIVTAAVDEKLNEVGYIVPGLGDAGDRLYGTVGSG"
     gene            1152..1691
                     /locus_tag="VGD55_10550"
     CDS             1152..1691
                     /locus_tag="VGD55_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEY0870827.1"
                     /translation="MSSDRRPGGSEVGSGSAGSADDAGRDFAVVCYREEGRWELGLLP
                     ERASSSLESLIAVLRQQPGEVTVGLVDIADDFFIAARLSGEDVRLLLSDVTAADEWRL
                     ARDVLEWLGIPEPTDDDLDDVVPVGDLALFSDLGLPEIELGLMLSDIDAYADEMLFSI
                     ARRLGFGDELERLVDAGVH"
     gene            1738..2265
                     /locus_tag="VGD55_10555"
     CDS             1738..2265
                     /locus_tag="VGD55_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEY0870828.1"
                     /translation="MSHFAALAAKTDDTWHSSEVELEGLADIDEVAEVMRDASVDDGP
                     VLLFVEQDDEWFAIVRVDGAGEPRTFISDARVVPGSELAALVFEEVPEDVVEELDDAS
                     LDDDDDVDVDEEVEEPAARPDAEPRGATDVLADFGISAAQLLAMCAEEGMLPADVITA
                     VCERLGCAEEIEVYR"
     gene            2235..2726
                     /gene="tadA"
                     /locus_tag="VGD55_10560"
     CDS             2235..2726
                     /gene="tadA"
                     /locus_tag="VGD55_10560"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY0870829.1"
                     /translation="MRRGDRGLPLIGSDESLMRLALDQASLALATGDVPIGAVVVDAA
                     GDVIGAGCNAREATGDPTAHAEIVALRAAAVSVGGWRLAGCTLVVTLEPCTMCAGALV
                     LARVDRLVFGAVDPKAGAVGSLWDVVRDRRLNHRPEVTGGVLEAECGELLRSFFEGRR
                     GVS"
     gene            2737..2826
                     /locus_tag="VGD55_10565"
     tRNA            2737..2826
                     /locus_tag="VGD55_10565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2771..2773,aa:Ser,seq:cga)
     gene            complement(2881..>3585)
                     /locus_tag="VGD55_10570"
     CDS             complement(2881..>3585)
                     /locus_tag="VGD55_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870830.1"
                     /translation="VAGLIILTRFREPTLHRTGQASRLREHLAATRQTVTQQPGVAPI
                     ALTMMLGAALTQMLFEFGPLWLVAAAVPTAFFGPYTAAMTSTLGLGGLLAGRVQLNRP
                     AVAVAVAVTMSGCGLALAVGGSGPLLIAAQVVLVGLLVTIGIHLSRQLHDLVPSQQRS
                     GVSSGIGTLSWLTFLPCSLLFAALSGNDGIRAAGWIITALVTVAGVTLVRIVTGPRTR
                     PMCADFLGTSTGAPTY"
BASE COUNT          580 a         1149 c         1250 g          606 t
ORIGIN      
        1 cgcgagcttg tcgatctgct cgagtcgcgg ggggtcgagg tcttctatgc gatccatccg
       61 gtggccggtc gcatgccagg ccacatgaac gtgctgctcg ccgaggcgaa cgtgccctac
      121 gagcaactca aagagatgga cgacgcgaac cccgagttca gctcgaccga cgtcgccgtg
      181 gttgtcggcg ctaacgatgt cgtgaacccg gcggcccgcg atgcacctgg cagcccgatc
      241 tacgggatgc cgatcctgaa cgtcgacgag gcaacgaacg tcgtcttcat gaagcgttcg
      301 atgcgccccg gcttcgccgg catcgacaac gcactgctct acaacgcgaa gaccacgctg
      361 ttcttcggcg atgcgaaaga ctcgctgtcg aagctcgtcg ccgcggtgaa ggccgtctag
      421 cccgagccga ccgttccgta caggcggtcg ccggcgtcac ccagacccgg cacgatgtag
      481 ccgacctcgt tcaacttctc gtcgaccgcg gccgtcacga tcgacagcgg caccttcgag
      541 tcggcgaagt gcgactccat gaacgcgaga ccctcgggcg ctgcgagcag gcagatggcg
      601 gtcacctggt cggcgttgcg ctcgaccagc aggtcgatcg ccgccgagag tgtgccgccc
      661 gtcgcgagca tcgggtcgag cacgtacacc tggcgaccgg cgagtgagtc ggggagtcgt
      721 gacgcgtagg tggtcgcgac cagcgtttcg cgatcgcgga ccagaccgag aaacccgacc
      781 tcggctgtcg gcaggagccg gcacatccct tcgagcattc caagcccagc ccgcaagacc
      841 gggacgacga ggggccgggg tcgcgccagc cgggtgccgc gcgcatgggc caccggcgtg
      901 acgacgtcaa cgtccgtcgt ccgcacgtcg cgcgtcgcct cgtaggcgag cagggtgact
      961 agctcgtcga cgaggcggcg aaaggtcggg gagtcggtgc gctggtcgcg cagggcggtg
     1021 agtttgtggg cgatcagcgg gtggtccgcc acgagggtgc gcatggcccg acacggtacc
     1081 gcgagctcgg gagccggatt gagtgcgtcc ggccattggc tcgtccggcg aacgcgtgcc
     1141 acgatgcagt catgtcgagc gatcgcaggc ccgggggtag tgaggtcggt tcgggctcag
     1201 ccggctcggc ggacgatgcc ggccgggact tcgcggtggt ctgttaccgc gaggaagggc
     1261 gctgggagct tgggttgctc cccgagcgcg cgtcgtccag cctcgaatcg ttgatcgccg
     1321 tgctccgcca gcagccgggt gaggtgacgg tcggcctcgt tgacattgct gatgacttct
     1381 ttatcgccgc gaggctgagc ggcgaagacg tccggctgct gctctccgac gtgacggccg
     1441 ccgacgaatg gcgcctggcg cgcgacgtcc tcgagtggct cgggatccct gagccgaccg
     1501 acgacgacct cgacgacgtg gtcccggtgg gtgatcttgc gctgttctca gatcttgggc
     1561 tccccgagat tgagttgggg ctcatgctgt ccgacatcga tgcgtatgcc gatgagatgc
     1621 tgttctcgat agcccgccgg ctcgggttcg gcgacgaact cgagcgcctc gtcgacgcgg
     1681 gcgttcacta gatccctacc gctccgatca gccacatcag ctttttcggg agattgcgtg
     1741 tctcacttcg ccgccttggc agccaagacc gatgacactt ggcacagttc tgaggtcgag
     1801 ctcgaaggtc tcgccgacat cgacgaggtc gccgaggtga tgcgtgacgc gtctgttgac
     1861 gacgggccgg tgctgctttt cgtcgagcag gacgacgagt ggttcgccat cgtgcgcgtc
     1921 gacggcgcgg gtgagcctcg cacgttcatc tcagatgctc gggtcgttcc tggcagcgaa
     1981 ctcgcggcac ttgtgttcga ggaagtacca gaagacgttg tcgaagaact cgacgacgcc
     2041 tccctcgatg acgacgatga cgtcgatgtc gacgaagaag tcgaggagcc tgcggcgcgt
     2101 cccgacgccg aacctcgcgg cgcgaccgac gtgctcgccg acttcgggat cagcgcggcg
     2161 caactgctgg cgatgtgcgc cgaagagggc atgctccctg ccgacgtcat caccgcggtc
     2221 tgcgagcgcc ttggttgcgc cgaggagatc gaggtctacc gctgatcggc tccgacgagt
     2281 cgctcatgcg gctcgcgctt gaccaggcct cgctcgcgct tgctaccggt gacgtgccaa
     2341 tcggtgcggt tgtggtcgac gccgccggtg atgtgatcgg tgccggctgc aacgctcgtg
     2401 aggctacggg cgaccccacc gcgcacgccg agatcgtggc cttgcgcgca gcggctgttt
     2461 ctgtaggcgg ctggcggctg gcaggctgca ccctggtcgt gactcttgag ccatgcacga
     2521 tgtgcgccgg cgccttggtg ctggcccggg tcgatcggct cgtgttcggc gcggtcgatc
     2581 cgaaggctgg cgcggtcggg tcgctctggg acgtcgtccg cgatcgccgc ctcaatcacc
     2641 ggcccgaggt cacgggcgga gtgctcgagg ccgagtgcgg cgagctgctt cggtcgtttt
     2701 tcgagggacg tcgaggggtc agctaggctc tcccgcggtg gagtcgcgta gtggccgagc
     2761 gcgcacgcct cgaaagcgtg tgagggtgaa agctctccgt gggttcaaat cccacctcca
     2821 ccgccaaatc gctcagccaa agcagccccc cgtttcatca cgtaaggggg gtgcgggtcc
     2881 tcaataggtg ggcgcacctg tcgaagtccc aagaaagtct gcgcacatcg gtctcgtcct
     2941 cggccctgtc acgatgcgga cgagcgtcac gcccgcgacg gtgacgagtg cggtgatgat
     3001 ccagccggcc gccctgatgc catcgttgcc gctgagggcg gcaaacagca gtgagcacgg
     3061 cagaaacgtc agccacgaca acgtgccgat gccggacgac acaccggacc gctgctgaga
     3121 aggcaccaag tcatgcagct gccgcgagag gtggatgccg atcgtcacca gcaggcctac
     3181 gagcacgacc tgggcggcga tcagcagcgg cccgctcccg ccaacggcca gggccagacc
     3241 gcacccgctc atcgtgactg cgacggcgac cgcaacggcg ggccggttca gctgcacgcg
     3301 ccctgcaagt agcccaccga gtccgagagt cgacgtcatc gccgccgtgt aagggccaaa
     3361 gaatgcagtc gggacggcgg cggcgacgag ccagagcggg ccgaactcga acaacatttg
     3421 ggtcagtgcg gctccgagca tcatcgtgag cgcgatcggt gcgacccctg gctgctgggt
     3481 caccgtctgc cgggtggcgg caagatgctc gcggagtcgt gatgcctgtc cggtccggtg
     3541 cagcgtgggt tcccggaagc gcgtcaggat gatcagcccc gcgac
//
LOCUS       DASWVF010000600         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_195353, whole genome shotgun sequence.
ACCESSION   DASWVF010000600 DASWVF010000000
VERSION     DASWVF010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195353"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VGD55_10575"
     CDS             <1..735
                     /locus_tag="VGD55_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEY0870831.1"
                     /translation="TTLPEWDFEARKDIYPLLLNVPYFDLYRRQVIKQADLEFALYTF
                     GNYFSDEDKARNVDYYEPRTVRDSSLSASVQAVVCAEVGHLELAHDYAYEAALIDLHD
                     IHNNTRDGLHIASLAGAWIALVAGFGGLRDTGGTLSFDPHLPHDISRLTFNLRWHGSR
                     LKVAVGRDEATYSLRDGTDGSITFRHGGASDTDGEDITLPSGESVTRPIAARPPLLPR
                     PKQPPGREPAPRPGSARDDTSGSPTS"
     gene            747..>1978
                     /locus_tag="VGD55_10580"
     CDS             747..>1978
                     /locus_tag="VGD55_10580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0870832.1"
                     /translation="MDRDALAVVPEAVPQIHPKASGGSFRALHSRPFRLYFAGQVASA
                     SGTFLQQTAIGWLVFRITGSASSLGLVLAAGGVPSLLLGPWGGSIADRFDLRKLLIVT
                     QSIFAALAATLWALAAAGSASVAVIVAVNVTSGVVSIVDSPARQAFVSRLVAPDDLAS
                     AVSLNGVVMNSARVIGPAIAGALIVTVGTTPCFAVNAISYAAVIVALAALHPLSSSPP
                     RGHGSKAGVRAGLRYARGRQQLWLPLLMMAIAGLLAFNFAVILPVLARDTFHGSGGTY
                     GLMSTMLSVGSIAGSLGVGFLHHPRRIYLVAASLAFGVALALTAAAPTVALACVALVV
                     TGATAFSYVTLSSTTLQLHSAPAYRGRIVALWVFVYIGTTPLGSALTGWICSTAGPRV
                     ALLVGAGACAVVAGVAARV"
BASE COUNT          283 a          690 c          623 g          382 t
ORIGIN      
        1 acgacgctgc ccgagtggga tttcgaggcg aggaaggaca tctacccgct gctgctgaac
       61 gtgccgtact tcgacctcta ccggcgccag gtcatcaagc aggcggatct ggagttcgcg
      121 ttgtacacct tcgggaacta cttcagcgac gaagacaagg cccgcaacgt cgactactac
      181 gaaccgcgga ctgtccgcga ttcgtcactg tcggcaagcg ttcaagccgt ggtgtgcgcc
      241 gaggtcgggc atctcgaact cgcacacgac tacgcgtacg aagccgcgct gatcgacttg
      301 cacgacatcc acaacaacac tcgcgacgga ctccacatcg cctccctcgc cggggcgtgg
      361 atagcccttg tcgccggctt cggcggcctg cgcgacaccg gcggcacgct cagtttcgac
      421 ccgcacctgc cgcacgacat ttcgcggctc accttcaacc tgcgctggca cggatcgcga
      481 ctgaaggtag cggtcggccg cgacgaagcg acgtactcgc tgcgcgacgg gaccgacggc
      541 tccatcacct tccgccacgg cggcgcgagc gacaccgacg gcgaggacat cacgttgccc
      601 agcggtgaat cggtcacccg ccccatcgcc gcacggccac cgttgttgcc tcggccgaaa
      661 cagccgcctg gtcgagagcc ggcaccccgg ccgggctctg cccgcgatga cacatcagga
      721 tcgccgacta gctagtttgt gtcgctatgg atcgcgacgc gcttgcggtg gtacctgaag
      781 cggtcccgca gatccacccg aaggcgtcag gcggctcgtt tagagcactt cattcgcgcc
      841 cgttccggtt gtacttcgcg ggccaggtgg cctcggcgag cgggacgttc ctccagcaga
      901 ccgccattgg ctggctggtc tttcggatca cgggatcggc gtcgtcgctc ggccttgtcc
      961 ttgccgcagg cggcgtcccg tcgttgctgc tcggaccttg gggcggctcg atagccgaca
     1021 ggttcgacct gcggaagttg ttgatcgtaa cgcaatcgat cttcgccgca ctcgctgcga
     1081 ccctgtgggc cctggctgca gccggctcag cgtcggtggc cgtcatcgtc gccgtcaacg
     1141 tcacgagtgg cgtcgtctcg atcgttgact caccagcccg gcaggccttc gtgagccgcc
     1201 ttgtggcgcc cgatgatctg gccagcgcgg tcagcctcaa cggggtcgta atgaacagtg
     1261 ccagggtgat cggccctgcg attgccggtg cgcttatcgt caccgtcggc acgacaccat
     1321 gtttcgccgt caacgccatc tcctatgccg ccgtcatcgt cgcgctggca gcgctccacc
     1381 ctctctcgag cagccccccg cgtggccacg gctcgaaggc gggtgtgcgg gccgggttgc
     1441 gctacgcgcg cgggcgtcaa caactgtggc taccgctgct gatgatggcg atcgccggcc
     1501 tgctggcatt caacttcgcg gtcattcttc cggtgttggc tcgcgacacc tttcatggct
     1561 ccggcggcac ttacgggctg atgtcgacca tgctcagcgt gggctcgatc gccggttcgc
     1621 tcggtgtggg cttccttcat catccgcgac ggatatatct cgtcgccgcg tcgctcgcct
     1681 tcggcgtcgc actcgccctg acggccgcgg cgccaaccgt tgcactcgcg tgcgtcgccc
     1741 tggtcgtgac cggcgcgact gcattctcct atgtcacgct gtcgtcgacg acactccagc
     1801 ttcattcggc gccggcctac cgcggacgca tcgtggcgct ctgggtgttc gtctacatcg
     1861 gcacgactcc gcttggcagt gcgctcactg gctggatctg ctcgacagcg ggcccgcgtg
     1921 ttgcgctgct cgtcggcgcc ggcgcgtgtg ccgtcgtcgc gggtgtagcg gcccgcgt
//
LOCUS       DASWVF010000601         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_246118, whole genome shotgun sequence.
ACCESSION   DASWVF010000601 DASWVF010000000
VERSION     DASWVF010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246118"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="VGD55_10585"
     CDS             complement(<1..2032)
                     /locus_tag="VGD55_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0870833.1"
                     /translation="MNVASGEVVGVEALARWQHPTLGLLFPDAFIPTAEHTSLIGPFT
                     SYVLDAALAQARIWADAGRPLPVAVNLSGRNLLDEHLPVEVADLLAAHAVPASLLELE
                     VTESAITLDPVRARRILQSLSDLGIRLSIDDFGAGYTSLSQLKMLPVDELKIDRSFVM
                     TMSSDARDALIVHSVVELARNLGLIVVAEGVETAQALVTLRGFGCDVAQGYHIARPVA
                     VEAFDAWLMARKPSADVPLDATPTRRASDAVLAERDFGEATSPAAALRASEERFRALF
                     TLAPIGILEARPDGTIVAVNPQACAMTGYDRDELIGRPATILIDPTDRAAQARSMASL
                     SNATAYTARRDYRRKDGTPLTVLISVAVVRQTSGAVSRMVGMMVDITELEAAQQKIAA
                     ASLELATNQMFTDSLLDNAGAGILACDTTGNVTVINRTARGWHALDPEVEPNAEQLAQ
                     LKEDLFELDGVTPLAVENNAMVRAFRDGITSKTEMVIAPAGGKATRVITTGMPLRGPV
                     GEVVGAVSTMHDVTLLRQRETELDQSIAFRDAVFAASRDVIFVADAATNRNLWSSRNL
                     TEFGYTDQQLKDLAGNTIDTLVHPDDRSRVREQNAAAQGLPDGEALRIRYRIRVDGGM
                     YRWFGRSVTPFARDAAGTVTQLLGVASDVAELVEVEERLIDAALHDSLTGLPS"
BASE COUNT          374 a          636 c          652 g          390 t
ORIGIN      
        1 gactgggtaa cccggtgagc gaatcgtgga gagccgcgtc gatgagtcgc tcttcgacct
       61 ccacgagttc ggcgacgtca ctggcgacac caagaagttg cgtgacggta cccgccgcgt
      121 cacgtgcgaa cggcgtgacg ctgcggccga accagcggta catcccgccg tctacgcgta
      181 ttcggtagcg gatccgcaag gcctcgccgt cgggcaatcc ttgagccgcc gcattctgtt
      241 cgcgaaccct agaccgatcg tcggggtgca caagagtgtc gatggtgttg cctgcgagat
      301 ccttcagctg ttgatcggtg tagccgaact ccgtgaggtt ccttgacgac cacaggttgc
      361 gattcgtggc tgcatcggcc acgaagatta catcgcggct ggccgcgaag accgcgtcac
      421 ggaaagcgat actctggtcg agctccgtct cgcgctggcg caataacgtg acgtcgtgca
      481 tggtgctgac cgcaccgacg acctcaccca ccggaccgcg caggggcata ccggtcgtga
      541 tcactcgcgt tgctttcccg ccggccggcg ctatgaccat ctcggttttc gaggtgatgc
      601 catcgcgaaa cgcccggacc atcgcgttgt tttcgacggc aagtggcgtg acgccatcga
      661 gctcgaagag atcctctttt agctgcgcaa gctgctccgc gttcggctcg acctcgggat
      721 caagtgcgtg ccagccacgt gccgtccgat tgatgacagt cacgttgcca gtggtgtcgc
      781 acgcgagaat tccagcaccc gcgttgtcga gtagcgaatc cgtgaacatc tgattcgttg
      841 ccagctcgag gctcgcggcg gcgattttct gttgtgccgc ttccaactca gtgatgtcga
      901 ccatcatgcc gaccatgcgc gataccgcgc ccgaggtctg gcgcacaaca gccacgctga
      961 tcagcaccgt aagtggtgtc ccatccttgc ggcggtagtc gcgccgcgcc gtatatgccg
     1021 tggcatttga tagcgacgcc atgctccgcg cctgcgcagc tcggtccgtc gggtcgatca
     1081 ggattgtcgc tggtcgcccg atcagttcgt cgcggtcgta gcccgtcatt gcgcatgcct
     1141 gtggattgac cgcgacgatc gtgccgtcgg gacgtgcttc caaaatgccg atgggagcaa
     1201 gcgtaaagag cgcgcgaaag cgttcttcgc tggcgcgtag tgcggccgcg ggcgaggtcg
     1261 cctcaccaaa gtcgcgttcg gccaggacgg catcgctcgc tcggcgcgtt ggtgtcgcgt
     1321 cgagtggtac gtctgcagac ggcttccgcg ccatgagcca ggcgtcaaac gcttcgacgg
     1381 ctaccggccg agcgatgtgg tagccctgcg caacgtcgca gccaaagcca cgaagcgtca
     1441 cgagcgcctg cgcagtctcg acaccttccg cgacgacgat gagtcccagg ttccgcgcga
     1501 gctcgacgac gctgtgcact atgagcgcat cgcgggcatc ggacgacatc gtcatgacga
     1561 aggatcgatc gatcttgagt tcgtcgaccg gcaacatctt caactgacta agactggtgt
     1621 agccggcgcc gaagtcgtcg atcgacagtc ggatgccgag atctgaaagg ctctgcagaa
     1681 tccgtcgcgc ccgcaccgga tcgagggtga tcgccgattc ggtgacctcg agttcgagca
     1741 gtgacgccgg cactgcgtgt gcagcgagca gatcggctac ctcgaccgga aggtgctcgt
     1801 caagaaggtt gcggccggaa aggttcaccg ccacaggcaa cggccggccc gcgtctgccc
     1861 agatacgagc ctgtgcgagt gcagcatcga gcacgtagct ggtaaaaggg ccgatgaggc
     1921 tggtgtgctc tgcggtcgga atgaaggcat ccgggaacag cagtcccagt gtcggatgct
     1981 gccagcgtgc gagtgcctcg acacccacaa cctcgccact ggcgacgttc actttgggtt
     2041 gaaagtgcaa ca
//
LOCUS       DASWVF010000602         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_67456, whole genome shotgun sequence.
ACCESSION   DASWVF010000602 DASWVF010000000
VERSION     DASWVF010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67456"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1642)
                     /gene="hflX"
                     /locus_tag="VGD55_10590"
     CDS             complement(59..1642)
                     /gene="hflX"
                     /locus_tag="VGD55_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0870834.1"
                     /translation="MTNIPSIKSSADAERFDAPADDDAPADAGSFDASADDDAPADAD
                     ASADDDAPERQADYVPSDFDGDFDGDQFDREDRQALRRVAGLSTELQDVTEVEYRQLR
                     LERVVLVGVWTEGTLRDAENSLVELKLLAETAGSEVLDGIIQRRDKPDPATYIGSGKA
                     KELRDIVLSTGADTVICDGELAPGQLRQLEEVVKVKVVDRTALILDIFAQHARSKEGK
                     AQVELAQMTYLLPRLRGWGESLSRQAGGQAAGGVGIGGRGPGETKIETDRRRIRARMA
                     KLRREIADMSTAREVKRQERKRGEVPSVAIAGYTNAGKSSLLNRLTGAGVLVENQLFA
                     TLDPTVRRAQTSDGRAFTLTDTVGFVRHLPHQLVEAFRSTLEEVAQADLIVHVVDGSD
                     TDPFAQLVAVREVLAEIDAQEVPELVVINKIDVADPETVDAVTRAEQRRGNRVVAISA
                     RTGAGIAELIAALDEMVPRIDVGIDVLVPFADGRLVARIHELGEVLTEEHTGDGTHIT
                     AKVPRRVAGELSAYAVTPA"
     gene            complement(1736..>2318)
                     /gene="dapF"
                     /locus_tag="VGD55_10595"
     CDS             complement(1736..>2318)
                     /gene="dapF"
                     /locus_tag="VGD55_10595"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0870835.1"
                     /translation="FARYLLDAGLVEPGRQTIATRAGVVTAWADLDGDITVDMGPPAI
                     SAGSTAVVDGHIYEGVAVSMGNPHLVCVTDVDVASLDLTAMPDVDVLAFPEGVNVEFV
                     NIVGGRHAVMRVHERGSGETQSCGSGACAVVAALAAIEGTNTGEYIVDVPGGRLIVSF
                     DDAGHLLLRGPAVLVAEGRFRVPGSPAIELGSA"
BASE COUNT          391 a          785 c          754 g          388 t
ORIGIN      
        1 ggctcgaccg cggcgcgcac ccaaaggcga gctagctcac gcctgcgccc gagctagctc
       61 atgccggcgt gaccgcgtag gccgacagct cgccagcaac gcggcgcggc accttcgccg
      121 tgatgtgagt gccatcaccg gtgtgttctt cagtcaggac ctcgccgagt tcgtggatgc
      181 gcgccaccag ccggccgtcg gcgaacggca ccagcacgtc gatgcccacg tcgatgcgcg
      241 gaaccatctc gtcgagcgcc gcgatcagct cggcgatgcc agcaccggtg cgggccgaga
      301 tcgcgacaac gcggttgccg cgacgctgct cggcgcgcgt caccgcatcg acggtctcag
      361 gatcggcaac atcaatcttg ttaatcacca caagctcggg aacctcttgc gcatcgatct
      421 cggcaagcac ttcgcggacg gcgaccagct gggcaaatgg atcagtgtcg gagccgtcaa
      481 ccacatgaac gatgaggtcg gcctgcgcga cttcttccag cgtcgaccga aatgcctcga
      541 ccagctgatg tgggaggtgt cgcacgaaac cgaccgtgtc ggtcagggtg aacgcccgcc
      601 cgtcggacgt ttgggcccgc cgcacagtcg ggtcgagggt ggcgaatagc tggttctcga
      661 cgagcacgcc ggcgccggtg aggcggttga gcagcgacga cttgccggcg ttggtgtatc
      721 ccgcgatcgc gaccgaaggg acctcgccac gcttgcgttc ctgccgcttg acctcgcgcg
      781 ccgtcgacat gtcggcgatc tcgcgccgca gtttcgccat gcgcgcgcgg atgcgacgcc
      841 gatccgtctc gatcttcgtt tcaccaggac cacgaccacc gatgccaaca ccgccggctg
      901 cttgaccacc ggcctggcgt gacagtgatt caccccagcc tcgaagtcgc ggcaacaggt
      961 aggtcatctg agccagctcg acctgcgcct tgccttcctt gctgcgggcg tgctgcgcga
     1021 aaatgtcgag gatcagtgcg gtgcggtcga cgaccttcac cttgacgact tcttcaagct
     1081 ggcgcagctg cccgggggcg agttcaccgt cgcagatcac cgtgtcggcc cccgtcgaga
     1141 gcacgatgtc gcgcaactcc ttggccttgc ccgagccgat gtaggtggca gggtcgggct
     1201 tgtcgcgacg ctggatgatg ccgtcaagca cctcagaccc ggccgtctcg gcgagcaact
     1261 tcagttcgac aagcgaattc tcggcgtcgc gcagcgtgcc ctctgtccag accccgacga
     1321 gcacgacgcg ctcgagtcgc agctgccggt actcgacctc ggtgacgtcc tgaagttcgg
     1381 tcgagaggcc ggcgacccgg cgcaacgcct gccggtcttc gcggtcgaac tgatcaccgt
     1441 cgaagtcgcc gtcaaaatcg ctgggcacat agtcggcctg acgctcgggc gcgtcgtcat
     1501 cagcggacgc gtcggcatca gcgggcgcgt cgtcatcagc ggacgcgtcg aagctcccgg
     1561 cgtccgcggg cgcgtcgtca tcagcgggcg cgtcgaacct ctcggcgtcc gcgctgctct
     1621 tgatgcttgg gatattcgtc atcgcctatc gatcgtgaca cgtttcggtc cgccgtgctc
     1681 agtgcatttg cccgcgacgg acaagttgga agggcgtgga cgctcaagag agcgttcagg
     1741 cgcttccaag ttcgatggcc ggtgagccag gcacgcggaa acggccttcg gcgaccaaga
     1801 ctgcggggcc gcgcaggagt agatggcctg cgtcgtcgaa cgaaacgatc aaccgaccgc
     1861 ccggaacgtc gacgatgtac tcaccggtgt tcgtcccttc gatcgccgcg agggcggcca
     1921 ccactgcgca cgcgccgctg ccacacgact gcgtttcgcc ggacccgcgt tcgtgcaccc
     1981 gcatgacggc gtgccgtccg ccgacgatgt tgacgaactc gacgttgacg ccttcgggaa
     2041 acgccagcac atcgacgtcc ggcatcgcgg tgagatctaa cgacgctacg tcaacgtcgg
     2101 tgacgcagac gagatgcgga ttgcccatcg agacggcgac gccttcgtag atgtgaccgt
     2161 cgacgacggc ggtcgagcca gccgagatcg cgggcggccc catgtcgacc gtgatgtcgc
     2221 cgtcgaggtc ggcccacgcc gtgacgacgc cggcgcgcgt cgcgatcgtc tggcgaccag
     2281 gttcgacgag gcccgcatcg agcaggtagc gcgcaaac
//
LOCUS       DASWVF010000603         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_93303, whole genome shotgun sequence.
ACCESSION   DASWVF010000603 DASWVF010000000
VERSION     DASWVF010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93303"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="cobA"
                     /locus_tag="VGD55_10600"
     CDS             complement(<1..1195)
                     /gene="cobA"
                     /locus_tag="VGD55_10600"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY0870836.1"
                     /translation="MNAGYPLLLDVAGRAVLVVGAGPVGARRATALADAGARVTVVAP
                     VVDEVLSGDNRIIFTRRAYEFSDLAGAWLIHACTGDDNVDSQIAADAEAAQVWCVRAS
                     SASSSAAWVPAVARVDDLTVAVNAGGDPGRAVAVRDAVRLLLETGELPLRRHRRAEAM
                     GSVALVGGGPGDPGLITVRGRRLLAEADVVVVDRLGPRALLDELAPDVEVIEAGKSPD
                     NHTLPQDAINELLISLARQGKRVVRLKGGDPFVFGRGGEELVALVRAGVACEVVPGVT
                     SAIAVPAAAGIPLTHRTMSRQFTVVTGHEDLDWSKLAAVEGTLVILMGATRVERVAKE
                     LIKAGRDPNTPVAVIENGTLPEQRTTTGSLDTIGGRATAVGVRAPAVVVVGDVATLHD
                     VAHDLV"
     gene            complement(1192..>1873)
                     /locus_tag="VGD55_10605"
     CDS             complement(1192..>1873)
                     /locus_tag="VGD55_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY0870837.1"
                     /translation="PLLEHLEAVPVGFDAWIQPARLPIQYVIRSTDYRGYAGQLASGV
                     IEIDDVVVIMPSGMRTTIVGIDTFDGEVPLAHAPQSVVVRLADDIDVGRGDLIVHPRQ
                     EPRVVREFDATVCQLSDRPLHAGDRVLIKHTTKVVKALVASVNHRLDVVTLEHDAADS
                     LGLNEIGGVTLRMAGPLAVDDYADDRLTGSFLVIDEHDGQTLAAGLVGDPIRRTSSAS
                     ASSGSETP"
BASE COUNT          331 a          660 c          600 g          282 t
ORIGIN      
        1 acaccaagtc gtgcgccacg tcgtgaaggg tcgctacgtc gcctacgacc acgaccgcag
       61 gtgctcgcac acccaccgcg gtcgcccggc cgccgatcgt gtcgagtgac cccgtcgtgg
      121 ttcgctgctc cggcagcgtg ccgttctcga tcacggcgac cggcgtgttc ggatcgcggc
      181 cggccttgat cagttctttc gcaacgcgct cgacgcgggt cgcacccatc aagatcacca
      241 atgtgccttc gaccgcggca agtttcgacc agtcgagatc ctcgtggccg gtgacgacgg
      301 tgaactggcg cgacatcgtc cggtgcgtca acgggatccc tgctgcggcc ggcacggcga
      361 tcgcacttgt gacacctggc acgacctcgc aggccactcc ggcccggacg agcgcgacca
      421 actcttcgcc gccacggccg aacacgaacg ggtcgccgcc cttcaacctg acgacgcgtt
      481 ttccttgcct agcaagggaa atcagcaact cgttaatggc gtcttgcgga agtgtgtggt
      541 tgtccggaga cttaccggcc tcaatcacct cgacgtcagg ggcgagctcg tcgagcaacg
      601 cacgcggacc tagacgatcg acgacgacga cgtccgcttc ggccagcaat cgccgtccgc
      661 gcacggtgat caagccggga tcaccgggac cgcccccgac gagcgcgacc gagcccatcg
      721 cttcggcgcg acgatgacga cgcagcggca gttcgccggt ttcgagcaac aagcgcacgg
      781 cgtcacggac ggccaccgcc cgaccggggt caccgcctgc gttgacggcg accgtgaggt
      841 cgtcgactcg ggcgaccgcc ggaacccatg ctgccgacga tgacgccgac gacgcgcgca
      901 cgcaccacac ctgtgccgct tcggcatcgg cggcaatctg agagtcgaca ttgtcgtccc
      961 cggtgcaggc atgtatcaac caggccccgg caaggtcgga gaactcgtat gcgcgccgcg
     1021 tgaagatgat gcggttgtcg cccgacagaa cctcgtcgac gaccggcgcg accacagtga
     1081 ctcgcgcacc cgcatcggcc agtgcggtcg cgcgacgagc accgaccggc cccgctccga
     1141 ccaccaacac cgcccgcccg gcgacgtcga gaagcagcgg atagccggcg ttcacggcgt
     1201 ctcgctaccc gaggaggcgc tggccgaaga cgttcggcgg atcgggtcgc ccaccagccc
     1261 ggcggcaagc gtctgcccgt cgtgctcgtc gatgacgagg aaactgccgg tcaaccggtc
     1321 gtcggcgtag tcgtcgacgg ccagcggccc cgccatgcgc agcgtcacgc caccgatctc
     1381 gttcaagcca agcgagtcgg ccgcatcgtg ctcaagtgtc acgacgtcaa ggcggtggtt
     1441 cacgcttgcc accaacgcct tgacgacctt agtggtgtgc ttgatcagca cccggtcgcc
     1501 ggcatgcagc ggtcgatcgg agagctggca gaccgtcgcg tcgaactcgc ggacgacccg
     1561 cggctcctgg cgaggatgaa cgatgagatc gccgcggccg acgtcgatgt cgtcggcgag
     1621 gcgcacgacg actgactgcg gcgcgtgtgc cagcggcacc tcgccgtcga acgtgtcgat
     1681 ccccacgatg gtcgtgcgca tccccgaggg catgatgacg acaacgtcgt cgatctcgat
     1741 gacacccgat gcgagttgcc cggcgtagcc gcggtaatcg gtcgacctga tcacatactg
     1801 aatcggcagc cgtgcgggct ggatccatgc atcgaagccg accggcaccg cctcgagatg
     1861 ctcgagcaag ggc
//
LOCUS       DASWVF010000604         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_475725, whole genome shotgun sequence.
ACCESSION   DASWVF010000604 DASWVF010000000
VERSION     DASWVF010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475725"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGD55_10610"
     CDS             complement(<1..628)
                     /locus_tag="VGD55_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY0870838.1"
                     /translation="MSLLVVGLSHRSAPLPLLERVSLPAEAAGKLLDDVVAAPAVDEA
                     MLLSTCNRVELYASVEKFHAGVAALSELLARHTGVDFDELSPHVYVHYEERAAQHLFA
                     VTSSLDSMLVGEHQILGQVRTAFRNAQDRGDAGSVLHEVVEHALHAAKRAHAETGIDT
                     AGSTLVEAGLQLGVEALRGKGASSIEADAGLAGRRAIVVGAGSMASVAA"
     gene            complement(625..1374)
                     /locus_tag="VGD55_10615"
     CDS             complement(625..1374)
                     /locus_tag="VGD55_10615"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY0870839.1"
                     /translation="MRANSPHGRSPGSRVSPNQASKNHASNQASNQAPSPPRGIPDAS
                     IARLPLYLRALQTMADRGVTTVSSHELAVASGVNSAKLRKDLSHLGSYGTRGVGYEVA
                     YLVYQISRELGLTQDWAVAIIGIGNLGRALANYGGFATRGFRTACLIDADPRQVGQEV
                     GGLVVSHLDDLEMLIAEHGIAIGVLATPAGAAQQVADRLVAAGIRSVLNFAPAVLTVP
                     PDVELRKVDLSTELQILAFHEQRRVSAGVSA"
     gene            complement(1438..1686)
                     /locus_tag="VGD55_10620"
     CDS             complement(1438..1686)
                     /locus_tag="VGD55_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY0870840.1"
                     /translation="MSTTAPRIALYTRPGCHLCEAAREVVEKVSADLGEPYVECDITL
                     DEDLLGRYVESIPVVEVDGVQVDFWRVSEERLRAALRR"
     gene            1862..2650
                     /locus_tag="VGD55_10625"
     CDS             1862..2650
                     /locus_tag="VGD55_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0870841.1"
                     /translation="MVTPRVRRASKWVATLSLVASALVGSAALASPAFASPAGDLGAA
                     TNASRTSNGLPALTVNASLTAVAQAWANQLAAANVLSHNPALRNQVSDWSVLGENVGM
                     AGDIPTVQAAFMASPPHRANILDPRYTQMGVGEASSIYPSCGCKVLWVVVDFRRPTTV
                     AAAPAPNATPTPVKPATAKTVTPAKPVTKVAPKASAKVVTGQVTDKVVTQSTTVAGVA
                     SGVVAASATALRTQLAASTDPAGASSDPVGRMLNFANVLSTLPG"
     gene            complement(2709..2807)
                     /locus_tag="VGD55_10630"
     CDS             complement(2709..2807)
                     /locus_tag="VGD55_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEY0870842.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLKRTRVQRRNKK"
     gene            complement(3061..3297)
                     /locus_tag="VGD55_10635"
     CDS             complement(3061..3297)
                     /locus_tag="VGD55_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870843.1"
                     /translation="MLKVRCVMGAVERPRDKALAEVRFLTVAEVAALMRVSKMTVYRL
                     VHSGELPAVRVGRSFRVPEQAVHDYLRDAFIEAG"
BASE COUNT          619 a         1180 c         1093 g          576 t
ORIGIN      
        1 tagccgcaac tgatgccatc gaccctgctc cgacgacgat cgcacggcgc ccagccaacc
       61 cggcgtccgc ctcgatgctt gacgcaccct tgccgcgcaa cgcctcgaca cccaactgaa
      121 gacccgcctc gacgagcgtg ctgccggccg tatcgatgcc ggtttctgca tgcgcacgct
      181 tagccgcatg caaggcatgc tcgaccacct cgtgcaaaac gctgcctgca tctccgcgat
      241 cctgcgcatt gcggaacgcc gttcttacct ggcccaaaat ctggtgctcg ccgaccagca
      301 tcgaatcaag gctcgacgtc accgcaaaca gatgttgcgc agcacgctcc tcgtaatgca
      361 catagacatg cggggagagt tcgtcgaaat cgactcctgt atgccgcgcg agcaactcag
      421 acaacgctgc gacaccggcg tggaacttct cgaccgacgc gtacagctcg acgcggttgc
      481 acgtcgacag caacatcgct tcgtcaactg cgggcgccgc gacgacgtcg tcaagcagct
      541 tccctgcggc ctcggcgggc aacgagacgc gttcgagcag cggtaacggc gcgctgcgat
      601 gggagaggcc gacgacgagc aggctcacgc cgacaccccc gccgagactc gtcgctgttc
      661 atggaacgcg aggatctgca actcagtcga gaggtcgacc ttgcgcaact cgacgtcggg
      721 ggggacggtc agcaccgcgg gagcaaaatt gagcacgctt ctgatacctg cggcgaccag
      781 gcgatcggca acctgttgcg cagcgcctgc cggcgtcgca agcaccccga tcgcgatgcc
      841 gtgctcggcg atgagcatct cgagatcatc gagatgcgag acgaccaggc cgccgacctc
      901 ttgcccgacc tggcgggggt cggcgtcgat gaggcaggcg gtgcgaaagc cgcgggtggc
      961 gaagcccccg tagttggcca gcgcccggcc gaggttgccg atgccgatga tcgcgacggc
     1021 ccaatcctgc gtgagaccga gctcgcggga gatctggtag acgaggtagg ccacctcata
     1081 gccgacgccg cgggtgccgt aagaacccag atgagagagg tccttgcgca gttttgccga
     1141 gttgaccccc gacgcgacgg ccaactcgtg cgacgagacg gtcgtgacgc cgcgatctgc
     1201 catcgtctgg agcgctcgca ggtagagcgg cagtcgggcg atcgaggcgt caggaatgcc
     1261 tctgggcggg gacggcgcct ggttcgaggc ctggttcgag gcgtggttct tcgaggcctg
     1321 gttcggggat acgcgggagc ccggggacct gccatgaggg ctgttcgctc gcactggggc
     1381 tccggactcg tcgggtcgtg cccagcgtac gtgtttgtga acacgcgcac aaaagcgtta
     1441 gcgacgcaga gcggcgcgca gtcgttcctc ggatacccgc cagaagtcga cttgaacgcc
     1501 gtcgacctcg accacgggga ttgattcgac gtacctgccg agcagatcct catcgagggt
     1561 aatgtcgcac tccacgtaag gctcaccgag gtcggccgac accttttcga caacttctct
     1621 agcggcttcg cacaggtggc agccgggccg cgtgtacagc gcgattcgcg gcgcggtcgt
     1681 cgacaccggc acacattaac ccgaaggtta ggccgatcgg cggtactgct gacctgcggc
     1741 gatacgccga ctgcgcgacg tcttcacaac cagtaggccg tcagggtgat cctgctccgc
     1801 aatgtccggt atgcggcttt tgcggttcaa gcgtcctgag cggtccgccg accaatacac
     1861 catggtcacc cctcgagttc ggcgtgcaag caagtgggtg gccacgcttt ccctggtcgc
     1921 atcagcgctc gtcggctcgg cggctttggc gtccccggcg ttcgcgagtc cggccggcga
     1981 ccttggagct gcgacgaacg cctcgcggac cagcaacgga cttcccgcgc tcaccgtgaa
     2041 cgcatcgctg accgccgtcg cgcaggcgtg ggccaaccag ctcgccgccg ccaacgtgtt
     2101 gtcgcacaac ccggcgcttc gcaatcaggt cagcgactgg agtgtcctcg gtgagaacgt
     2161 cggcatggcc ggcgacatcc cgaccgtcca ggcggccttc atggcaagtc ccccgcatcg
     2221 ggccaacatc ctcgaccccc gctacaccca gatgggtgtc ggcgaggcgt cttcgatcta
     2281 cccctcgtgc ggctgcaagg tgctgtgggt cgtcgtcgac tttcggcgcc cgacgacggt
     2341 cgcggcagcg cctgccccca acgccacccc gaccccggtc aagcccgcga cggcgaagac
     2401 cgtgacgccc gccaagccgg tgaccaaggt cgcgcccaag gcctcagcca aggttgtgac
     2461 gggtcaggtc acagacaaag tcgtgacgca aagcacgacg gtcgccggtg ttgcttctgg
     2521 tgtcgtggct gcgtcggcaa cggccctgcg cacccagctc gcggcatcga ctgatccggc
     2581 cggggcaagc agtgaccccg tcggacgcat gctgaacttc gccaacgtgt tgtcgacgct
     2641 gccgggctga cccagcccta gatcttgtcg cgccgctact gcttgcgtcg ctacttcttg
     2701 ttgcgtcgct acttcttgtt gcgtcgctga acgcgggtgc gcttcagcag cttgcggtgc
     2761 ttcttcttcg ccatccgctt gcgacgcttc ttgatgaccg aacccacaac gaacccactt
     2821 cccacgggga cagtgcccct gacacgacgt gctcgaaaag atctgaaaga catgccttga
     2881 tcgccaccgg ccgaccagag gccgacgccg cacgcgcctc acggccctga cagccctgac
     2941 ggcgaggcag cgatcgtgcg acaccgccac cgtgaggcct tgtcggccct gcggctcaca
     3001 tcagcgtagc tgtaacccgc gtcgcccgac cgcgctgcga atgtgcaccg agacgcgcgg
     3061 tcagcctgct tcgatgaacg cgtcgcgcaa gtagtcgtgg accgcttgct cgggtacccg
     3121 gaatgagcgg ccgacccgaa cggcgggcaa ctctcccgaa tgcaccaagc gatagacggt
     3181 cattttcgac acgcgcatga gcgcggcgac ctccgcgacc gtgaggaacc gcacttcggc
     3241 cagcgctttg tcgcgtggcc gttcaactgc acccatgacg caccgaactt tcagcacgcg
     3301 acgggtgccg gcttccccac cgacagacga tcacgcacgt gcgtgaccga gggtagcgat
     3361 gttggggtca gtggggaagg gatcgtcccc cgaggcgaca aaatgcaccg tcggggagcg
     3421 tctgtcacat attcggcaac gctgcgtgac gatcgaacgc gccctcac
//
LOCUS       DASWVF010000605         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_67512, whole genome shotgun sequence.
ACCESSION   DASWVF010000605 DASWVF010000000
VERSION     DASWVF010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67512"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VGD55_10640"
     CDS             complement(<1..1569)
                     /locus_tag="VGD55_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870844.1"
                     /translation="MSTPNASLLPVAGGAEVKAYAASLIRKQRRAVGGVLALYSLAGF
                     AGLAGPRLLGDIVGAVQHGTTRAHIDRIAMILAGFVIAQAILVRLARYASSVIAEGIF
                     AELREEFLRKVISLPLSTVERAGTGDLLTRMTSDIDAMSRSVRFAMPETMIAVATAVL
                     TLLAAFITAPLVALPCLAVFPLLFVATRWYLKRAPQGYLRERRVYSHMFAGMTETLEG
                     GRTIDAMRLRDKRIRRGDDDVRRAYVAERYTLRLRTVWFPSTEAAYVVPVVGSLLVGG
                     LLAIHGRASLAQVTAVTLYFQQLVDPVDRLLGWLDELQVGASSLARLVGVGRVPADRT
                     ATGAEPDGEELRAQGVTFAYRAGRDVLHGVDLDVRPGERIAMVGPSGAGKSTLGRLLA
                     GIHGPGDGSVTVGGVPLVDLEIERLRREVALVTQEHHVFVGSVADNMRLAKPSADDDQ
                     LRSVLQSIDALDWVTALPEGLGTMVGSGGHILTPAQAQQIALARLVLADPHTLVLDEA
                     TSLLDPRAARRLERS"
     gene            complement(1566..>2595)
                     /locus_tag="VGD55_10645"
     CDS             complement(1566..>2595)
                     /locus_tag="VGD55_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870845.1"
                     /translation="FARYQTRSQDVRRKGIAVAAVGSVLDAAEVLLPGVFVVLITWLG
                     ARLALQHKLTVGQLVSFYGYAAFLLAPMRIATEVADRFTRGLVAAKRVVKVLELQPTL
                     PAASQPVPVPRQGELVDTTSGLVVRTGDFVAVAAGEPAEASALADRLGRYRDPDPGEK
                     VLFDGVPLTEMSLDDVRAAALIVDKDPRLFAGQLRDQLDPHRQAGTLGVQEAIDAAVA
                     QDAVDSVPGGLDGVLDERALGLSGGQRQRIALARALLADPQILILDEPTSAVDAYTEA
                     RIADTLRAHRAGKTTIIMTTSPLLLDRADRVVLLQGDRVVAEGHHRDLITSDARYRSV
                     VTRDEETA"
BASE COUNT          455 a          859 c          889 g          392 t
ORIGIN      
        1 tgaacgctcg agccgtcgag ccgcgcgcgg gtcgagcaac gacgtcgcct cgtcgaggac
       61 caatgtgtgg gggtcggcaa gcacgagacg cgccagcgcg atctgctgtg cctgcgcggg
      121 cgtcaagatg tggccacccg agcccaccat ggtgccgaga ccttcgggca gtgccgtgac
      181 ccagtcgagc gcgtcgattg actgcaagac agaccgcaac tggtcgtcgt ccgccgaagg
      241 ttttgcaagg cgcatattgt cggcgacaga accgacgaag acgtggtgct cctgcgtcac
      301 gagcgcgacc tcgcgtcgca gtcgctcgat ctcgagatcg acgagaggaa cgccgcccac
      361 tgtcacgctc ccgtctccgg gaccgtggat cccggcgagg aggcggccga gtgtgctctt
      421 gcccgcgccg ctcgggccga ccatggcaat gcgctcgccc ggacgtacat cgagatcgac
      481 gccgtgcagc acgtcgcggc ccgcgcggta ggcgaatgtg acgccttgcg cacgcaactc
      541 ttcgccgtcg ggctcggcgc cagttgcggt gcggtcggcg ggtacccggc cgacgccgac
      601 gagccgcgcg agtgacgacg cgccgacctg gagctcgtcg agccagccga gaagtcggtc
      661 gaccggatca acgagttgct ggaagtagag cgtcaccgca gtcacctgag ccaggctcgc
      721 gcgcccatgt atcgcaagca ggccaccgac gagcagcgag ccgacgaccg gaaccacgta
      781 cgcggcctcg gtgctcggga accacaccgt gcgcaggcgc agtgtgtagc gctcggcgac
      841 gtacgcgcgc cggacgtcgt cgtcgccacg ccggatccgc ttgtcgcgca gccgcatcgc
      901 atcgatggtg cgcccgcctt cgagcgtttc cgtcatgccg gcgaacatgt gcgagtagac
      961 gcgccgctcg cgcaggtagc cctgcggtgc tcgcttcagg taccagcggg tcgcgacaaa
     1021 cagcaacgga aatacggcca ggcagggcaa cgcgaccagt ggcgccgtga tgaaggcggc
     1081 aagcagggtg aggacggcgg ttgcgaccgc gatcatcgtt tccggcatcg cgaaccgcac
     1141 gctgcgcgac atcgcatcga tgtcactcgt catgcgggtg agcaggtcgc cggtgccggc
     1201 acgttcgacg gtcgacaagg gcagcgagat gactttgcgc aggaattcct cgcgcagctc
     1261 ggcaaaaatg ccttcggcaa tgacggacga cgcgtagcgg gcaagccgca cgaggatcgc
     1321 ctgcgcaatc acgaagccgg ccaggatcat cgcgatccgg tcgatgtgcg cgcgggtggt
     1381 tccgtgctgc accgcgccga cgatgtcacc gagcagccga gggccggcca ggccggcgaa
     1441 ccccgcgagg gagtagaggg caagcactcc accaacagcg cgacgttgct tgcgaatcaa
     1501 gctcgcggcg taggccttga cctcggcgcc ccccgctacc gggagtagtg acgcgttagg
     1561 ggtgctcatg ctgtttcctc gtcgcgtgta acaaccgagc ggtatcgcgc atcactggtg
     1621 atgaggtcgc ggtggtgccc ctcggcgaca acccggtcgc cctgcagcag cacgacccgg
     1681 tcggcgcggt cgagcagtag cggactcgtg gtcatgatga tcgtcgtctt gccggcacga
     1741 tgcgcccgca gcgtgtcggc gattcgcgct tctgtatagg cgtcgaccgc actcgtcggt
     1801 tcgtcgagga tcaggatctg cggatcggcg agcagtgcgc gggcaagggc gatccgttgg
     1861 cgttggccac ccgacagccc cagtgcccgc tcatcgagta cgccgtcgag accaccaggc
     1921 accgaatcga ccgcgtcttg cgcgaccgcg gcatcgatgg cttcttgaac accgagcgtt
     1981 ccggcctggc ggtgggggtc gagttggtcg cgcaactgtc cggcgaacag gcgcgggtct
     2041 ttgtcaacga tcagggcggc ggcgcggacg tcgtcgagcg acatctcggt aagcggcact
     2101 ccatcaaaga gcaccttctc gccggggtca ggatcgcggt agcggccgag gcgatcggcg
     2161 agtgcgcttg cctcagcggg ttcgcccgca gccacggcga cgaagtctcc cgttctgacg
     2221 acaaggcccg aagtcgtgtc gaccaattct ccttgtcgtg gcaccggcac cggctgcgat
     2281 gccgccggca acgtcggctg cagctcgagg accttcacga cccgcttggc cgcgacgagc
     2341 cctcgagtga acctgtcggc gacctcagtc gcgatgcgca tcggcgcaag caggaacgct
     2401 gcgtacccat aaaacgagac cagctggccg accgtaagtt tgtgctgaag cgcgagccgg
     2461 gcgccgagcc acgtgatgag gaccacgaag acgcccggca gcaagacctc cgcagcgtcg
     2521 agcaccgagc cgaccgccgc gaccgcgatg cctttacgcc tcacatcttg cgagcgcgtt
     2581 tggtagcgcg caaag
//
LOCUS       DASWVF010000606         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_246207, whole genome shotgun sequence.
ACCESSION   DASWVF010000606 DASWVF010000000
VERSION     DASWVF010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246207"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..513)
                     /locus_tag="VGD55_10650"
     CDS             complement(139..513)
                     /locus_tag="VGD55_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870846.1"
                     /translation="MSHATATQSVMMEVPGVGAFVRALLPINLTGEHKLTYGVWLAID
                     PRELPGVFDVWWEPGYAQLQLAGLLANVVQPWGMLGSPVLTEVRDPDQTPYCASSTDP
                     TLNGVLTEHWDHDLVLGPIGNT"
     gene            1093..>1950
                     /locus_tag="VGD55_10655"
     CDS             1093..>1950
                     /locus_tag="VGD55_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0870847.1"
                     /translation="MTSLPRPHDDGGRDPDVWLSRRVAGLALRLVVLVVPLLVAVATS
                     FVVTRIVHAPKNFWPRTLWLAGVVAACGLLVVPARRVLRRLLPLAALLELAIRFPGAA
                     PTRWKVARQAGTIRHLELLAAGVPDTEPVKAATTILALVASLSRHDRVTRGHSERVRV
                     FTDVIAQEMALSRADRDRLRWAALIHDVGKLEVPGRLLRKPGKPSPQEWETLRLHPQV
                     GARLAAPLNGWLGSFGAVVSQHHERFDGTGYPLGLAGEEISVGARIVGLADAFEVMTA
                     ARPYKRAMSR"
BASE COUNT          285 a          602 c          687 g          376 t
ORIGIN      
        1 agggcatcga gcagtgccgc gtgcgctggc ttcgtcaccg tgagacgttc gttcacgctt
       61 tacaggatcc accatctcgc gtggtgaaca tccgcctttg ccgcatggcg cgcgacgaga
      121 ctcgcgggct tcttggtctt aggtgttccc gatcgggccg agcactaggt cgtgatccca
      181 gtgctccgtg aggacgccat tcagggtcgg gtcggtggag ctcgcgcagt agggtgtttg
      241 gtcggggtct cgaacttcgg tcagtaccgg tgatccgagc attccccacg gttgcaccac
      301 gttagcgagt agaccggcta gctgaagttg ggcgtagccc ggctcccacc acacgtcgaa
      361 cacacccggc agttcacgcg gatcgatcgc aagccacacg ccgtaggtga gcttgtgttc
      421 cccggtcagg ttgatcggca gtagtgctcg gacgaacgcc ccaacgccgg gtacctccat
      481 catcacggac tgcgtggcgg tcgcgtggct catccaagtg ccgacggttc gctcctgatc
      541 cggcagtgcc aagacggggt caggtagacc gaatcgaaca tgacgatcgt gggcgtctac
      601 cgcgcggccg cacgacgggc agtgcagggt gtccaccgcc ctagtatcca gccgcgtccg
      661 actcctcggg gtgaggtcga gcgtctgagc gtcaacctca acgcactgat ctgccgcgag
      721 cggtgcgtcg tcagccagat gttcgggagt catgcggcgg ggtcgtttcg gtaggagatg
      781 atgcgccggt gaccctgtcg ggcgtgagat gacaacactc ccgatccgcg cgcgggtgcc
      841 gacggcggcg gggactcgat gccgtatgac gtgtatgggt atctgcgtgg aggcccgagg
      901 gcggcgctgg tctcggcgtt ggcggtgatg catacgaggg ggctgatcgg ggctgggcgg
      961 cccggaatga cgcttggcgc gagcatttgc cgctggtcgc gcgcttcggc gcggctccgt
     1021 gagcccaacg gccgaccgat gatgagccag tcggcggtgc gcgatttcgc agaatgctcc
     1081 gatctttcca gggtgacgag tttgccgcgc cctcatgacg atggcgggcg cgatcccgac
     1141 gtgtggcttt cgcgccgtgt cgctggcctc gccctgcggc tggtcgtgct ggtcgtgccg
     1201 ctgctcgtcg cggtcgcgac gtcgttcgtc gtgactcgta tcgtgcacgc gccgaagaac
     1261 ttctggccgc gaactctgtg gctcgccggg gtcgttgctg cgtgcggcct gctcgtcgtt
     1321 ccggctcgga gagtgttgcg gcggctgctg ccgttggcgg cgctgcttga gctggcgatc
     1381 cgtttccctg gagcagcacc gacgaggtgg aaggttgccc gtcaggccgg cacgatccgc
     1441 caccttgagc ttctcgcagc aggcgttccc gacaccgaac cggtcaaggc ggcgacgaca
     1501 atcttggcac tggtcgcctc gctctcgcgc catgaccgtg tgacgcgcgg gcattcagag
     1561 cgagtgcgcg tcttcaccga cgtgatcgcc caggagatgg ctctttcgcg cgcggacaga
     1621 gatcgattgc gatgggccgc gttgatccac gacgtcggca agctcgaagt tcccggtcgg
     1681 ctactccgca agccagggaa gccgagcccg caggagtggg agacactgcg cctgcatccg
     1741 caagttggag cccggctcgc tgctccgctg aacggttggc tcggctcgtt tggtgcggtc
     1801 gtcagccagc accatgagcg gttcgacggc accggctatc cgttggggct tgcgggcgag
     1861 gagatctcgg tcggtgcccg catcgtcggg ctcgcggacg cgttcgaggt gatgacagct
     1921 gcccgtccat acaaacgggc gatgtcgcgg
//
LOCUS       DASWVF010000607         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_348151, whole genome shotgun sequence.
ACCESSION   DASWVF010000607 DASWVF010000000
VERSION     DASWVF010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348151"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1603
                     /locus_tag="VGD55_10660"
     CDS             242..1603
                     /locus_tag="VGD55_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870848.1"
                     /translation="MSARSARHTPDAHFDNGRAGSALRAVRQLITSTLAVIALAASGS
                     AALAAAGSTVFAGTAVAATATPIAVIAPANVGLFGSGDPTYDGVYRQSLAIIGLVATG
                     HTPNAPAINWLLSQQCADGSFTAYRADPTKACTAASEDENSTALAIQALTALGKPTAT
                     AIAALKHFQLADGGFYDNTAFGPAASDANSTGLAESAFVAAGIDPSTVTSTGGKTADD
                     YLRSIQIACSAASGPGAYDFQGEATLHANDYASVQALLGELGKTLTVGVAAAGGSVPA
                     CPATITTAATSAAAATSYLAARLAASNGQIPSSLGSGIDWTTTANAVLDLEAAGAGNA
                     SVAAALTALETNVKAYVQPAGTFVPGPLATLLLVAHASGIDPGAFGGADLTTSLATTE
                     RVAVTPVVAPAPAPTTTPTTTALPMTGATDVRPIGSVAALLVGLGIGALAVSRRRTRA
                     PRG"
     gene            1608..2504
                     /locus_tag="VGD55_10665"
     CDS             1608..2504
                     /locus_tag="VGD55_10665"
                     /inference="COORDINATES: protein motif:HMM:NF040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO2322 family protein"
                     /protein_id="HEY0870849.1"
                     /translation="MIRAASWVVAALSCLAGVAAFTVATPAPASASTAYRYWAFYLAH
                     GSTWQYSQRGPASEYPVDGDVEGWRFAIQVDAATGLSPRQVPDFAALCASTPAKAGEI
                     RVGLVIDFGVAIDAPAHESPPAGVVPGCIYVHAGDSGAAVLQAATTVRIGTGSRVGLV
                     CGIDGYPKTECTPAVTAPTASTPGAHPSTTPSSTPPQNAQAPTAPPAPLSSAAPASAS
                     SITPGGSIDGSLTPSDPASLLSSSASAIVPLGAAGQPTSLASLRSTSHSTDPLEYTAL
                     IGVVLVVVIGGAAFWRTRRGQP"
BASE COUNT          395 a          945 c          863 g          404 t
ORIGIN      
        1 attctcgggc ccttcagtca cctcgaagga aatcgtcgag tgaggcggca agccggatta
       61 cctgccggat gcgaattggc tccaatcgcc tgccgaggtt cgcggggcgg agcccgggtc
      121 gctcgacccg ccatcgcccc cacgtcgcgc cgtctgcgca cgtggggttt gcgttgtcgg
      181 gagcggacgg cggacgccgg gatccatcgc gttgatcgcg gcgcatcccg agaggaattc
      241 gatgtccgca cggtccgcac gtcatacccc agacgcacac ttcgacaacg gccgggccgg
      301 gtctgccctt cgcgccgtgc gtcagctgat cacgtcgaca ctcgccgtca tcgcgctggc
      361 agccagcggg tctgccgccc tagccgccgc cggatccacc gtcttcgccg gcactgcagt
      421 agcggcgacc gccacaccga tcgccgtcat cgccccagcg aatgtcgggc tcttcgggtc
      481 gggagacccg acctacgacg gcgtctaccg gcagagcctc gccatcatcg gcctggtggc
      541 caccggccac acacccaacg cccccgccat caactggctg ctgtcgcagc agtgcgccga
      601 cggctcgttc accgcgtatc gagccgaccc cacgaaggcc tgcacggcgg cttcggaaga
      661 tgagaactcc acggcgctgg cgatccaggc tttgacggcg cttggcaagc cgaccgcgac
      721 agcgatcgcg gctctgaagc acttccagtt ggccgacggc ggcttctacg acaacaccgc
      781 tttcgggccc gccgcctcag acgccaactc gactggactt gccgagagtg cgttcgtggc
      841 tgccggcatc gatccctcga ccgtcacgtc gacgggcggc aagacagccg acgactacct
      901 gcgctcgatc cagatcgcct gctccgcggc gtccggtcct ggtgcgtatg actttcaggg
      961 cgaggcgacc ctgcacgcaa acgactacgc gagcgtccag gcgctgctcg gtgagttggg
     1021 caagacgctg acggttgggg ttgcggcggc cggtggttcg gtgcctgcgt gccctgcgac
     1081 gatcaccacc gcggcgacct cggccgccgc agccacgagc tatctcgcgg cacgactcgc
     1141 ggcgtcgaac ggccagattc cgtcgtcact gggaagcgga atcgattgga cgacgacggc
     1201 gaatgcggtg ctcgaccttg aggccgcggg tgctggaaac gccagcgtcg ccgccgcact
     1261 cacggcactg gaaaccaacg tgaaggcgta cgtgcaaccg gccgggacgt tcgtgcccgg
     1321 accactggcg acgttgttgc tcgttgcgca tgcgagcggg atcgaccccg gtgcgttcgg
     1381 tggcgccgac ctcaccacga gcctcgccac gaccgagcgg gtcgccgtaa caccggtcgt
     1441 cgcacccgca cccgcgccga ccaccacacc cacgacaacg gcgctgccga tgaccggagc
     1501 caccgacgtg cgacccatcg gctcggtcgc agctctgctc gtcgggctcg gcatcggcgc
     1561 cttggcggtc agccgcaggc gcactcgggc accgcgggga tgaccgcgtg atccgagccg
     1621 cgtcgtgggt ggtcgcggcc ctgtcatgcc tcgccggcgt cgcggccttc acggtcgcga
     1681 cgccggcacc cgcgagcgcc tcaaccgcct accgttactg ggctttctac ctcgctcacg
     1741 gcagtacgtg gcagtactcg cagcgtggac cggcgagcga atacccagtc gatggggacg
     1801 tcgaaggctg gcgtttcgcg atccaggtcg atgctgcgac cggactctcc ccgcggcaag
     1861 tgcccgactt cgcggcgctg tgcgcatcga ctccggcgaa ggccggcgag atccgggtcg
     1921 ggctcgtgat cgactttggg gtggcaatcg acgcgccggc tcacgagtcg ccgcctgccg
     1981 gcgtggtgcc gggatgcatc tacgtgcacg ccggtgacag cggcgccgcg gttttgcagg
     2041 cggcgacgac ggtacggatc ggcaccgggt ctcgcgttgg cctggtctgc gggatcgacg
     2101 gctacccgaa gaccgagtgc acaccagcgg tgacggcgcc gacagcttct acgcccggtg
     2161 cgcacccatc gacgacacct tcgtcgacgc ctccgcagaa tgcgcaagca cccaccgcgc
     2221 ctccggcacc gctgtcgtcg gcagcgccgg cgtccgcgag ctcgatcacc cccgggggct
     2281 cgatcgacgg ctcgctcaca ccaagcgatc cagcgtccct tctgagcagt tccgcgtcgg
     2341 cgatcgtgcc ccttggcgcg gccgggcagc cgacctcact cgcgtcgttg cgatcgacga
     2401 gtcacagcac cgacccgctg gagtacacgg cgttgatcgg cgtcgttctt gtcgtcgtga
     2461 tcggcggcgc cgcattctgg cgcacccgac gtgggcaacc atgacgctga acctccgccg
     2521 cccgcgagag gctcacatcg cgcgatctgc acgagcgctg catccgggag cgtggtgggc
     2581 ctggtccctc gcactgggag cagccgc
//
LOCUS       DASWVF010000608         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_297014, whole genome shotgun sequence.
ACCESSION   DASWVF010000608 DASWVF010000000
VERSION     DASWVF010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297014"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VGD55_10670"
     CDS             complement(<1..994)
                     /locus_tag="VGD55_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0870850.1"
                     /translation="MTTTSGALAVRTVVLPDAEERDFFVVDGRITFEKPHYLDVETLA
                     DRGFALPGLVDAHCHVGLGAQGGTPIDVSREHAIADRDGGALLLRDAGVPETHYALDG
                     EADLPRIVHAGRHLARTRRYIRGYALELEPGQLPDAAAAQAVRPGGASTPWVKIVGDW
                     IDREVGDITPLWHPDELTEAVRRAHDAGARVGVHVFGEEALPDIIAAGVDSIEHGTGL
                     DASMLDEVARRGIAVVPTMVMVSQFDGFAEQATRYPVYAARMRRLKQSADARMMATFD
                     AGVPIYVGTDAGGHLPHGLAVDEMLLMHGAGMPNIDVLAAGSWKARQWLGLPGIV"
     gene            complement(991..2601)
                     /gene="ffh"
                     /locus_tag="VGD55_10675"
     CDS             complement(991..2601)
                     /gene="ffh"
                     /locus_tag="VGD55_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0870851.1"
                     /translation="MFDTLSDRLESVFKTLRSKGRLSDADIDATTREIRVALLEADVA
                     LPVVREFIDAVKERSRGASVSAALNPAQQVISIVNEELVHILGGETRRLRYAKNPPTV
                     IMLAGLQGSGKTTLAGKLAKWLQSQGHAPLLVAADLQRPNAVEQLQVLGKQAGVPVFA
                     PEPGSGVGNPVAVAKAAIEHAKRLNHDIVVVDTAGRLGVDAEMMQQAINIRDAVDPDE
                     ILFVVDAMVGQDAVNTAQAFLDGVGFDGVVLTKLDGDARGGAALSVASLTGRQIMFAS
                     TGEKLDDFDIFHPERMASRILGMGDILTLIEQTQGAYDAGEAEETARKLSEGDLTLED
                     FLEQMMMLRKMGPIGNLLGMLPGMGQMKEQIAQVDDKDIDRIAAIIRSMTPIEREDPK
                     IINGSRRVRIANGSGVTVAEVNSLVDRFFEARKMMKQMAGMGGLPGMGARRATKAVKK
                     GKKGRGGRPGGRPGGVPAGLARTLPGGEGPAGIGGGMGMPGQLPGQLPGQLPPGMKMP
                     DLSKLRFPPTGGTGSKGGNQGPAGPRPR"
     gene            complement(2638..>3934)
                     /locus_tag="VGD55_10680"
     CDS             complement(2638..>3934)
                     /locus_tag="VGD55_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0870852.1"
                     /translation="LATKCPPMPIPWPASARDAFIALLGAGRPALVVFEALDQAGLMA
                     RLIPEWESVRYRRQHNPLHRYTVDRHLVEAAIEAAALTRRVARPDLLLLGALFHDIGK
                     GSDGDHSIAGAALMPGIAARIGLNDRDSAVLVTLVRYHLLLSQTATRRDLDDPATVAA
                     VAKAVGSPLVLELLHALTEADARATGTPMWDDWRARLVADLVRRTQLVLAGETVPLPG
                     APGADLPAAIIKLAELGQLAVISESTSEHSRITVVAPDQPGLLWRSAGVLALHRLGVR
                     AASATSIGPTAVTVFDVEPTYFSEVDPARIQDDMRRAIEGGLDVAQKLARREATAATR
                     KSIALPPATVLLPGGASDLATVLEVRAHDRPGLLFTVTRVLAAEGLDVRSARVETLGA
                     EVVDAFYVTDRAGRPLSSERAETVRHGVEEALRGSGPRA"
BASE COUNT          656 a         1352 c         1269 g          657 t
ORIGIN      
        1 cgacaatgcc tggcaatccc aaccactgcc gggctttcca cgaaccggcg gcgagtacgt
       61 cgatgtttgg catgccggcg ccgtgcatca aaagcatctc gtcgacagcg agaccgtgcg
      121 gcaggtgccc acctgcatcg gtgccgacgt agatcggcac gccggcatcg aacgtcgcca
      181 tcatccgcgc atctgcactc tgcttcagcc gacgcatgcg ggccgcgtac accggatacc
      241 tcgtcgcttg ctcggcaaag ccatcgaact gcgacaccat caccatcgtc ggcaccaccg
      301 cgatgccgcg ccttgccacc tcgtcgagca tcgatgcgtc aagaccggtg ccgtgctcga
      361 tcgagtcgac accggcggcg atgatgtcag gcagcgcttc ttcaccgaac acatgcacac
      421 cgacccgcgc gccagcatcg tgcgcgcgac gaactgcttc agtaagttcg tcggggtgcc
      481 atagcggcgt gatgtcaccg acctcgcgat cgatccaatc gccgacgatc ttcacccacg
      541 gtgtgctggc gccgccgggt cgaactgcct gtgcagcagc ggcatcgggc aactgcccag
      601 gctcgagttc gagcgcatac ccacggatgt aacggcgggt gcgggccagg tggcgaccgg
      661 cgtgcacgat gcgcggcaag tcagcttcgc cgtcgagcgc gtagtgggtc tcgggtactc
      721 cggcatcgcg caacagcagt gcgccgccat ctcgatcggc gatcgcgtgc tcgcgcgaca
      781 catcgatcgg cgtgccaccc tgcgccccca ggccgacatg gcagtgcgca tcgaccaagc
      841 cgggtaacgc gaaaccgcgg tcggccaatg tctcgacatc gagatagtga ggtttctcga
      901 acgtgatacg gccgtcgacg acgaagaagt cgcgctcctc ggcgtccggt aagacgacag
      961 tacgaacggc gagtgccccg ctcgtagtgg tcaacgcggc ctcggaccgg ccggcccctg
     1021 attgccaccc ttgctacccg tgccaccggt cggcgggaac cgaagtttcg acaggtcggg
     1081 catcttcatg ccagggggaa gttgtccagg caactgcccg ggaagttggc ccggcatgcc
     1141 catgccgccg ccgattccgg ccgggccctc accgcctgga agcgttcgag caagccctgc
     1201 agggacgccg cctggtcgcc cgcctggcct gccgcccctg cccttctttc cttttttcac
     1261 tgccttggta gcgcgccgcg cacccatgcc gggcagcccg cccatgccgg ccatttgctt
     1321 catcatcttg cgagcttcga agaagcggtc gaccaaactg ttgacctcgg caaccgtcac
     1381 acccgaaccg ttcgcgatgc gcacccgacg tgagccgttg atgattttcg ggtcctcgcg
     1441 ctcgatcggc gtcatcgacc tgatgatcgc ggcgatccgg tcgatgtctt tgtcgtcgac
     1501 ctgcgcgatc tgctccttca tttggcccat gccgggcagc atgccgagca ggtttccgat
     1561 cgggcccatc ttgcgaagca tcatcatctg ctcgagaaag tcttcgagcg tgaggtcgcc
     1621 ttcgctgagt ttgcgggcgg tctcttcggc ttcacctgcg tcgtaggcac cttgcgtctg
     1681 ctcgatcagc gtcaagatgt cgcccatgcc gaggatgcgc gacgccatgc gctcgggatg
     1741 gaagatgtcg aagtcgtcga gcttctcgcc ggtgctcgcg aacatgatct ggcggccggt
     1801 caacgaagca accgaaaggg ccgcaccacc gcgcgcatcg ccgtcgagct tggtgagcac
     1861 aacgccgtcg aagccgacgc cgtcgaggaa cgcctgcgct gtgttgacgg cgtcctggcc
     1921 gaccatcgcg tcgacgacga acaggatctc gtcggggtcg acggcatcgc gaatgttgat
     1981 ggcttgctgc atcatctcgg cgtcgacgcc aaggcgacca gccgtgtcga cgacgacgat
     2041 gtcgtggttc agtcgcttcg catgctcgat cgcggccttg gccacggcaa ccggattgcc
     2101 gacgccgctg ccaggctcgg gtgcgaacac cggcacgccg gcctgcttgc cgagcacttg
     2161 caactgctcg accgcgttcg gccgctgcag gtcggccgcg acgagcagtg gtgcgtgccc
     2221 ttgcgactgc aaccacttcg cgagcttgcc agcgagggtg gttttacccg agccctgaag
     2281 accggcgagc atgatgaccg tcggtgggtt cttcgcatag cgaaggcgcc gagtttcacc
     2341 gccgaggatg tggacgagtt cctcgttgac gatcgagatg acctgctgtg ccgggttcag
     2401 cgcagccgag acggatgccc cgcgtgagcg ctctttgacc gcgtcgatga attccctgac
     2461 gaccggcagc gcaacgtccg cctcgagcag ggccacccgg atctcgcgag tcgtcgcgtc
     2521 gatgtcggcg tccgacagcc gtcccttgct gcgcagggtc ttgaagaccg actcgagccg
     2581 gtcagagagg gtgtcgaaca cgcgcgccag cctaccgact gcgcgtgcag cgtgccgtca
     2641 cgcccgcggc ccgctgcctc gcaacgcttc ctcgacgccg tggcgtaccg tctccgcacg
     2701 ctcgctcgac aacggccggc ccgcgcgatc ggtgacgtag aaggcgtcga cgacttcggc
     2761 gccgagcgtc tcgacccggg ccgaccgcac gtcgagacct tcggccgcga gcaccctggt
     2821 gaccgtgaac agcaacccgg gccggtcatg ggcgcgcacc tcgagaacgg tggcgagatc
     2881 tgatgccccg cccgggagca gcacggtcgc aggcggcagc gcgatcgact tgcgggtcgc
     2941 cgccgtcgct tcgcgacggg ccagcttctg tgcgacgtcg aggccacctt cgatggctcg
     3001 tcgcatgtcg tcctggatgc gtgccggatc gacctcggag aagtacgtcg gctcgacgtc
     3061 gaagaccgtg accgcggtcg ggccgatcga cgtcgcggac gcagcgcgga cgccgagccg
     3121 gtgcagcgcg agcactccgg ccgaccgcca caacagcccg ggctggtcgg gagccacgac
     3181 cgtgatgcgg ctgtgctcgg acgtgctctc ggagatcacc gccaactgac caagctcggc
     3241 aagcttgatg atcgcggccg gtagatcggc gccgggcgca cccggcaacg gaacggtctc
     3301 accggcgagc accaactgcg tgcggcgcac caagtcggca acgagacgcg cacgccagtc
     3361 gtcccacatc ggggtgccgg tcgcccgtgc gtcggcctcg gtcaatgcat gcaacaactc
     3421 gagcaccagc ggtgagccca cggctttcgc gaccgccgca accgtcgcgg ggtcgtcgag
     3481 atcgcgtcga gtagcggtct gcgaaagcag caggtggtaa cgcaccagcg tgacgagcac
     3541 cgcgctgtcg cgatcgttca gcccgatgcg cgctgcgatg cccggcatca acgcggcgcc
     3601 ggcgattgag tggtcgccgt cgctgccctt gccaatgtcg tggaagagcg cgccgagcaa
     3661 cagcaggtcg gggcgcgcga cccgacgtgt caacgccgcc gcctctatcg ctgcctcgac
     3721 gagatgccgg tcgactgtgt agcggtgcag cggattgtgt tgccggcggt agcgaacgct
     3781 ctcccactcc ggaatcagcc gagccatcag acctgcctga tcgagagctt cgaacaccac
     3841 cagcgctggg cgacctgcac ctagcaacgc gatgaacgca tcgcgtgccg atgctggcca
     3901 cgggatcggc atcggcgggc atttcgttgc cagc
//
LOCUS       DASWVF010000609         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_42355, whole genome shotgun sequence.
ACCESSION   DASWVF010000609 DASWVF010000000
VERSION     DASWVF010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42355"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="rplB"
                     /locus_tag="VGD55_10685"
     CDS             complement(<1..666)
                     /gene="rplB"
                     /locus_tag="VGD55_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0870853.1"
                     /translation="MGIRKFKPTTSGRRGASVADFVEITRTVPEKSLTRPLHSKGGRN
                     VHGRVTVRHQGGGHKRAYRLIDFRRDDKDGVPAKVAHIEYDPNRTARIALLHYADGEK
                     RYIIAPARLQQGDTVEAGPSSDIKPGNTLPLRNIPVGTVVHAIELRPGGGAKIARSAG
                     TSVQLVAKEGAYAQLRMPSGEIRNVDVRCRATVGEVGNAEQSNINWGKAGRMRWKGKR
                     PTVR"
     gene            complement(700..999)
                     /gene="rplW"
                     /locus_tag="VGD55_10690"
     CDS             complement(700..999)
                     /gene="rplW"
                     /locus_tag="VGD55_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY0870854.1"
                     /translation="MTIPDPRDVLYAPLISEKSYGLLDENKYTFLVDPDANKSEIKIA
                     VEKVFGVKVTDVNTMNRKGKRKRTRGGFGKRKDTKRAIVTLVAGDRIDIFGGPVS"
     gene            complement(996..1604)
                     /gene="rplD"
                     /locus_tag="VGD55_10695"
     CDS             complement(996..1604)
                     /gene="rplD"
                     /locus_tag="VGD55_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0870855.1"
                     /translation="MPAEVFDVQVNIPLIHQVVVAQLAAARQGTHDAKTRGEVSGGGR
                     KPYRQKGTGRARQGSTRAPQFAGGGVAHGPNPRNYEQRTPKKMKAAALRGALSDRARG
                     GRLHVLPGFGEGEAPSTKAALSALASVVTGDQHRVLIVLERNDDQTWKSLRNVDGVHL
                     LVEDQLNTYDVLVADDVVFTEGALQTYVKRATGAAAVEEEQK"
BASE COUNT          240 a          532 c          521 g          313 t
ORIGIN      
        1 acggacggtc gggcgcttgc ccttccagcg catgcggccg gccttgcccc agttgatgtt
       61 cgactgctcc gcgttgccga cctcaccgac ggttgcccgg cagcgcacgt caacgttacg
      121 gatttcgccg gacggcatac gcaactgcgc gtaggcgcct tccttggcga cgagctgaac
      181 actggtgcct gccgagcgcg cgattttcgc cccaccaccg ggccgaagct caatggcatg
      241 gacgacggtg ccaaccggga tgttgcgcag cggcaaggtg ttgcccggct tgatgtcact
      301 gctcggaccg gcctcgacgg tgtcgccctg ctgcaacctc gccggcgcga tgatgtagcg
      361 cttctcgccg tcggcatagt gcagcaaagc gatgcgcgcg gtgcggttcg ggtcgtactc
      421 gatgtgcgcg accttggcgg gcacgccgtc cttgtcgtca cgacggaagt cgatgagccg
      481 gtaggcacgc ttgtgaccgc caccctggtg gcggaccgtg acccggccgt gcacgttgcg
      541 accacccttg gaatgcagcg gccgcgtcag cgacttctcc ggcaccgtcc gagtgatctc
      601 gacgaagtcg gcaacgctgg cgccacggcg accggaggtc gtcggcttga acttgcggat
      661 acccatcgct tcttttcctt cagctcatta gtaggccggt caggagaccg gaccaccaaa
      721 gatgtcgatg cggtcgccgg ctaccagcgt cacgattgcc cgcttggtgt ccttgcgctt
      781 gccgaatccg ccgcgggtgc gcttgcgctt gcccttgcgg ttcatcgtgt tgacgtcggt
      841 gaccttcacg ccgaagacct tttccaccgc gatcttgatt tcgctcttgt tggcgtcagg
      901 gtcgaccagg aacgtgtact tgttctcgtc gagcaagccg tagctcttct ccgagatgag
      961 cggcgcgtag agcacatcgc gcgggtcggg gatcgtcatt tctgctcctc ctcgactgca
     1021 gcggcacccg tcgcgcgctt cacataggtc tgcagcgcac cttcggtgaa caccacgtcg
     1081 tcggcaacca acacgtcgta ggtgttgagc tggtcttcaa cgagcaggtg cacgccgtcg
     1141 acgttgcgca ggctcttcca ggtctggtcg tcgtttcgct cgagcacgat cagaacacgg
     1201 tgctggtcac cggtcacgac cgacgccagc gccgagagtg cggccttggt ggaaggcgct
     1261 tcgccctcac cgaatccggg cagcacgtgc aggcggccac cgcgcgcgcg gtcggacaac
     1321 gcgccacgaa gtgcggcggc cttcatcttc ttcggcgtcc gctgctcgta gttgcgcggg
     1381 ttcggaccgt gtgcgacgcc accgccggcg aactgcggag cacgggtcga gccctggcgg
     1441 gcgcggccgg tgcccttctg gcggtacggc ttgcgacctc caccagacac ctcgccacgg
     1501 gtcttcgcgt cgtgcgtacc ttggcgggcg gcagcgagtt gcgcgaccac gacttggtgg
     1561 atcaacggaa tgttgacctg cacatcgaag acctcggcgg gcagct
//
LOCUS       DASWVF010000610         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_501236, whole genome shotgun sequence.
ACCESSION   DASWVF010000610 DASWVF010000000
VERSION     DASWVF010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501236"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VGD55_10700"
     CDS             <1..497
                     /locus_tag="VGD55_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0870856.1"
                     /translation="WFAGGGRPRPRRGDVRLAVLTLLAEKPMHGYQIITELGTRSGGV
                     WRPSPGSVYPTLQQLQDEGLVTVSEEDGRRTFSLTDAGRAEVERASAGRRAPWEDMAE
                     EADDASGRLKGLVGQLIAATLQVATAGSHDQVAQAEKVLTDARRAIYRLLAEDEPKDP
                     KAQS"
     gene            494..904
                     /locus_tag="VGD55_10705"
     CDS             494..904
                     /locus_tag="VGD55_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEY0870857.1"
                     /translation="MTTAIRIGDAEREAAISSLTSHFAEGRLTQVEHDERTTLALHAK
                     TGRDLDVLFADLPRRGEATPATRRPARGFRIERLVQPLFAALFIGVMIFVILNLLPTI
                     ALVILAVVACRLLFGFRRHHWYRSQRWYGPPRRW"
     gene            complement(905..1333)
                     /locus_tag="VGD55_10710"
     CDS             complement(905..1333)
                     /locus_tag="VGD55_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY0870858.1"
                     /translation="MTITNEWNAKIVADFRENEGRVGGNFEGAPITILHHVGRKSGRE
                     YLAPVMYLPADDNPDTIYVFASAGGAPVHPKWYDNMIAAGTATVEVGTQSYPVTVTDV
                     TGAERDRVYAEQVKRYPGFGEYESKNVGIRTIPVLKLTRA"
     gene            1388..1768
                     /locus_tag="VGD55_10715"
     CDS             1388..1768
                     /locus_tag="VGD55_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0870859.1"
                     /translation="MMSDSRPAFVLHEHHKPRHHFDLRLESDGVLRSWALPRGLPPDS
                     THDRLAVPVDDHELDHLTYTDADKSIADIGWWEQHDANEKRIVFTLHGATSDVRYALI
                     NTGGDWLLHRVKQQVSGSAGGAGA"
     gene            complement(1745..2350)
                     /locus_tag="VGD55_10720"
     CDS             complement(1745..2350)
                     /locus_tag="VGD55_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870860.1"
                     /translation="MNDNDILDRVTASASGLHMETPVDAIVARGHVRRRRRVSGTAAV
                     GVIAAVGLAVGLVTVEGDQHPESSGGVQLASFTLVSNTDGTATLTLVKGVPLDADGLR
                     HKLADAGIPAIVNVGRACDSHPQPAGLDQVVSPQRRSDGSVVLTITPSAMPAGAELSI
                     GVFPTGRTWSLATIGAPLTCETTVPQTSGSGSEGSGPRPAS"
     gene            complement(2343..2900)
                     /locus_tag="VGD55_10725"
     CDS             complement(2343..2900)
                     /locus_tag="VGD55_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0870861.1"
                     /translation="MREAIVSQQPATFVDFYQASRDGCLRAVMVSTGDRQLAEELVAE
                     AFARAWASWPKVGRHPAPAAWVVRTALNTGISWWRKRRREFPLEGHDAPVSTELEGVD
                     SEVMNALRRLPARQREVVALRIFLDLDTETTARALGIAPGTVTAHMSRAITALRRELA
                     PSNFERGMSVTPGFLMTEEKRQRNE"
     gene            complement(2917..>3710)
                     /gene="miaA"
                     /locus_tag="VGD55_10730"
     CDS             complement(2917..>3710)
                     /gene="miaA"
                     /locus_tag="VGD55_10730"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0870862.1"
                     /translation="VDLAHRMNGEIINADSMQLYQGMDVGTAKLTQDERQGVVHHLLD
                     IWPVTRTASVADYQTHARAAIADIAARGRTPILVGGSGLYIRAVIDDMRFPGTDADVR
                     ANLETELAAVGAPAMHARLAAVDAATAATMLPTNGRKIVRALEVAAIGGGSFTGLMPP
                     YESVYDALLIGVDRPTAELDERITQRVATMWERGLVEEVRTLELAGLRDGVTARRALG
                     YRQVLAMLDGELSEEQARAQTVQATKRFVRRQRSWFRPDPRINWL"
BASE COUNT          623 a         1216 c         1198 g          673 t
ORIGIN      
        1 cgtggttcgc gggcggcggg cgaccaaggc cccggcgcgg cgacgtccgc ctcgccgtgt
       61 tgacgctctt ggccgagaag ccgatgcacg gctaccagat catcaccgag ctcggcacgc
      121 gcagcggcgg tgtgtggcgc ccgagccctg gctcggtgta cccgacgctt cagcaactgc
      181 aggacgaggg tctcgtcacg gtttccgagg aagacggcag gcgaactttt tcactgacgg
      241 acgccgggcg ggctgaggtc gagcgcgcgt ctgcaggtcg tcgggccccg tgggaggaca
      301 tggccgaaga ggccgacgac gcatccggcc ggctcaaggg cctcgtcggg caactgatcg
      361 cagcgaccct tcaggtggca actgcaggca gccacgacca ggtcgcgcaa gccgagaagg
      421 tgctcaccga tgcacgccgg gcgatctacc ggttgctcgc cgaggacgaa ccaaaggatc
      481 cgaaggcgca gtcatgacaa ctgccatacg cataggggac gccgagcggg aggccgcaat
      541 ttcctcgctc acaagccatt tcgccgaagg acgactgacg caggtcgaac acgacgaacg
      601 gacgacgctc gcgttgcacg caaagaccgg tcgcgatctc gacgtgctgt tcgccgatct
      661 cccgcgacgt ggcgaagcaa cgccggccac tcggcgtccc gctcgaggat ttcgcattga
      721 gcgactggta cagccactgt tcgcggcgct attcatcggc gtgatgattt tcgtgatcct
      781 gaacctgctg ccgaccatcg cgctggtgat actcgcggtc gtggcatgcc ggctgctatt
      841 cgggtttcgc cgtcaccact ggtacaggtc gcaacggtgg tacggaccgc cgcgtcgttg
      901 gtagtcaggc gcgcgtcagc ttgagcaccg ggatcgtgcg gatgccgacg ttcttgctct
      961 cgtactcgcc gaacccgggg tagcgcttga cctgctcggc gtacacccgg tcacgctcag
     1021 cgccggtgac gtcggtgacc gtgaccgggt agctctgggt tccgacctcg acggttgccg
     1081 tgcccgcggc gatcatgttg tcgtaccact tagggtgaac gggtgcgccg cctgccgatg
     1141 cgaagacgta gatcgtgtcg gggttgtcgt cggcgggcag gtacatgacc ggggcgaggt
     1201 actctcggcc actcttacga ccgacgtgat gcaggatcgt gatgggtgcg ccctcgaagt
     1261 tgcctccgac acggccttcg ttttcccgga aatcggcgac gatcttggcg ttccactcgt
     1321 tggtgattgt catggaggtc tcccgtcgat caagatttga ttgcgccttc aatcgcaacg
     1381 gctcccgatg atgagtgatt cccggccggc gttcgtcctg cacgagcacc acaaaccacg
     1441 ccaccacttc gacctgcgcc tcgagtccga cggagtgctc cgttcctggg cattgccccg
     1501 aggtctgccg ccggattcga cccacgaccg tctcgccgtg cccgtcgatg atcacgagct
     1561 ggaccacctg acctacaccg atgccgacaa gtcgattgcc gacatcggct ggtgggagca
     1621 gcacgacgcg aacgagaagc ggatcgtgtt caccctgcat ggtgcaacgt ccgacgtgcg
     1681 ctacgccctc atcaacaccg gcggcgactg gctcctgcac agggtcaagc agcaggtgag
     1741 cggctcagct ggcggggcgg gggcctgagc cttcgctgcc ggatccactc gtttggggca
     1801 ccgtggtttc acacgtcagc ggggcgccga tcgttgccag gctccacgtg cgacctgtcg
     1861 ggaagacgcc gatgctcagc tccgcacccg caggcatcgc ggacggggtg atggtcaaga
     1921 caacggaccc gtcggagcgc cgctgcggcg aaacgacctg gtcgagacct gccggctgcg
     1981 ggtgcgaatc gcaggcgcga ccgacgttaa cgattgccgg aatgccggcg tcggccagct
     2041 tgtgacgcag tccgtcggcg tcgagcggaa cccccttgac cagggtcaaa gtggccgtgc
     2101 cgtcggtgtt gctgaccaac gtgaacgacg cgagttgcac accgccactg ctctcagggt
     2161 gctggtctcc ttcgaccgtc accaggccga ccgccaatcc gacggccgcg atgaccccga
     2221 ccgctgccgt tcccgacacc cgacgccgac gtcgcacgtg acctctcgcc acgatcgcat
     2281 cgacgggtgt ttccatgtgg agccccgacg cagacgcggt cacccggtcg aggatgtcat
     2341 tgtcattcat tgcgttgcct cttctcttca gtcataagaa atccgggagt gacgctcatt
     2401 ccccgttcga aattgctggg cgcaagttcg cgacgcagcg ccgtgatcgc gcgggacatg
     2461 tgcgccgtca cggttcctgg ggcgatacct agtgcgcgtg cggtggtttc ggtatcgaga
     2521 tcgaggaaga tccgcaacgc gacaacctcc cgctgacgtg ccggcaggcg ccggagcgca
     2581 ttcatcacct cggaatcgac gccttcgagt tctgtcgaga ctggggcgtc gtgcccttcg
     2641 agcgggaact cgcgacgccg cttgcgccac cacgagattc ctgtgttgag tgcggtgcgg
     2701 acgacccacg cggccggtgc cggatgccgg cctactttcg gccacgacgc ccacgcacgc
     2761 gcgaatgcct cggcgacaag ctcctcggca agctgacgat ctccagtgct gaccatcacc
     2821 gctcgcaggc atccatcgcg gcttgcctga tagaagtcga cgaatgtcgc tggctgctgg
     2881 ctcacaattg cttcacgcat gagaagcccg attcacttac aaccagttga tgcgcgggtc
     2941 gggtcgaaac catgatcgct gcctgcgcac gaacctcttc gtcgcctgca ccgtttgtgc
     3001 gcgcgcctgc tcttctgaca actcaccgtc gagcatcgcg agcacctgcc ggtatccgag
     3061 cgcgcgtcgg gcggtcactc cgtcgcgcag tcccgcgagt tcgagggtgc ggacttcttc
     3121 gacgagtccg cgctcccaca tcgtcgcgac tcgctgcgtg atccgctcgt cgagctcggc
     3181 ggtcggccgg tcgacgccga tgagaagagc gtcgtaaacg gattcatacg gcggcattaa
     3241 accggtgaac gaaccaccgc cgatggccgc cacttcgagt gcgcgcacga tcttgcgtcc
     3301 gttggtcggc aacattgtcg ctgcggtcgc cgcgtcgacg gcagccaggc gggcgtgcat
     3361 tgccggcgca cccacggcgg cgagttcggt ttcgagattc gcccgcacat cggcgtccgt
     3421 gcccggaaaa cgcatgtcgt cgataaccgc gcggatgtac agacccgagc caccgacaag
     3481 tatcggcgtt cgtccccgag cagcgatgtc ggcgatcgcg gccctcgcgt gcgtctggta
     3541 atcggcgacg cttgcggtgc gtgtgacggg ccagatatcg agcagatgat ggacgacgcc
     3601 ctgccgctcg tcctgcgtca gtttcgcggt gccgacgtcc atgccctggt acagctgcat
     3661 cgagtcggca ttgatgatct cgccgttcat gcgatgtgcg aggtcgacac
//
LOCUS       DASWVF010000611         6063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_67577, whole genome shotgun sequence.
ACCESSION   DASWVF010000611 DASWVF010000000
VERSION     DASWVF010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67577"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..823
                     /locus_tag="VGD55_10735"
     CDS             143..823
                     /locus_tag="VGD55_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870863.1"
                     /translation="MPVTDAGNLAHNAVTALNTVKVVLNTYQQIQIMQSQLRYQLQSL
                     ETLNPSSFSGLLAAINQSETTYAMLQGDLTTMGFTVSQINRDFDRLFPRSQTQWNSVR
                     YSDFNGYYDRWNGELETSAKAAARAQAGISVLDASNRQIASILAQSNTASSGEVRQLQ
                     LMNQQLAVLHADLGSILQNLATTGRVLTDWTAGGVGEQMLARERARRRLDGYTSRGKP
                     SRVLKALP"
     gene            827..1915
                     /gene="trbL"
                     /locus_tag="VGD55_10740"
     CDS             827..1915
                     /gene="trbL"
                     /locus_tag="VGD55_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEY0870864.1"
                     /translation="MDVNVLTTVLTTFVNALATGLHSVQSGAGGIMRSLAAIEIVLCG
                     FWIALDGGERLSTICKFLLQLTFWMFFAANFAALAKVFSDTLIQYGLSASGQSGNVSL
                     LLDPSRIAGMAFDATQPLIQSMDDAGLTHMKDAIILGLCYLLIMACFFVIACQMCLAI
                     VEYYLIVTLSTCLVPFGISKHTRFIAEKAIGAVVSVSVKLMVLGFIVGLIQPVLAQLR
                     FSGGGEIKLNEVMSMILVCGLVAVVVWRAPGFAADLLAASPSLSAAAVGQHVTGAVSN
                     ATRAASGALSAGVGATRNAATLVRAGATGTAGAFRMVGSAMARGWQGSTTAAAAGGDR
                     ELAGAFGSRGATQPKAAARTPPPPTKSV"
     gene            1950..2660
                     /gene="trbF"
                     /locus_tag="VGD55_10745"
     CDS             1950..2660
                     /gene="trbF"
                     /locus_tag="VGD55_10745"
                     /inference="COORDINATES: protein motif:HMM:NF010446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HEY0870865.1"
                     /translation="MRLRPSSWEPAAPLDTPYRRARQEWDRRIGGAVVQASNWRRATF
                     AVLAVVALEAIGLVVLGAQPKAVPHLIQVDKLGAAAYLGPLERASLRDFQPPPASFEY
                     HLRRFVSDTREISSDVAVVQRNWLDAYRLVTQNGANQLNAYVRDHDPISRSENKARVS
                     VQVDVVVPISRESWQVDWTETLWDEHGGEIETSAWRATFRIVLRIPQTDAELKTNPIG
                     LYIDEVHWARLADGRNAK"
     gene            2657..3619
                     /gene="trbG"
                     /locus_tag="VGD55_10750"
     CDS             2657..3619
                     /gene="trbG"
                     /locus_tag="VGD55_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEY0870866.1"
                     /translation="MIRRLSLFAVFVSFLTGCAGRQPAPRYVRAEPVKDDPPKPTVVQ
                     VPHPIPVPGQLKPAPATNRRRASSPKHPSDIIREANRKATEGPSADDYYNAIMTYDYT
                     PGTLYQVYTAPQKLTDVQLQSGEHIVGKPATGDNIRWVLARGSSAVGPNEQQHIYIKP
                     TRPDLDTTLCINTDRRSYILELHSYDEAWMAAVAWRYPQDEVAQLESTLDQQQMLAQS
                     STPTGVSIDKLNFGYGIAVLSGRPQWVPVQVFDDTHKTYIRFPAAMLDREAPALFVVS
                     SSGDTQLVNYRVKNDTYIVDRLFEDAELRLGQQTQEIVRIVRTR"
     gene            3627..4985
                     /locus_tag="VGD55_10755"
     CDS             3627..4985
                     /locus_tag="VGD55_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEY0870867.1"
                     /translation="MDDTTKRDIQPSSNAAQSADEMTGSRPATNPSAGHAAPGPAALT
                     APSQFTYASKLPADHPGLQLDVQKGRTLRRGPVVTGVAIVAGATAIALVVAFSPSKDG
                     AKAASATPSAESSPPEIVLPDSIKNPTPAPRTPAMGAAQQDPTPASGAAGGRQHLREA
                     GTRRSGAGADERNRILASPILVDVDNPVQTESGVHPPVLGVPAAGLLSADRAAAPPPS
                     SASASVAGQGDANMQQHKSDFLTRDGLNSSTYVSQPVLAPRSPYEVKAGTIIPTSLIT
                     GINSDLPGQIVGQVRENVYDTVTGNYLLIPQGSRLLATYDSAVTFGQQRVLVCWNRLI
                     RPDGISIGLECMPGVDLSGYAGFADDVDHHWFRIITGVALGTLLSATAQRSQGDVSGY
                     NPTVPQLWAANAGSSVNQAGQAITQKNLSIQPTITVRPGYSVNVLVSKDIVLPPYNRA
                     DR"
     gene            5066..5836
                     /locus_tag="VGD55_10760"
     CDS             5066..5836
                     /locus_tag="VGD55_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0870868.1"
                     /translation="MNREHISQAELAGRLGQKPELDQTSGGVTFARLNIATSERYTDR
                     SGEIREKTEWTRAVAWGDLAESIANRFDKGSPIVLAGAMRVNSYEKDGAKNRVLELHV
                     DSAEPNLEAGTCRNEARLVGAVRSVESKTLDSGTAMTVLSVGTTTKQNGKDREDWHSV
                     TLWGRTAESGAKQIAVGDLISVNGSIRHRSVPGADGIERRLSAIDGRQFQVLERAKER
                     DHDQAAPAKTPDLASEAGGAAPPRARSRQRGKSVERDM"
BASE COUNT         1088 a         2116 c         1850 g         1009 t
ORIGIN      
        1 ctgactgaag cggcggacca gttggcccag ctcgcccggc gcaccggccc ctatccctcg
       61 tgaggtgacc atgcgtctaa ccctgttcgc cgcgttgatt ttcgtcgccc tcaccctgga
      121 gtcgtccccc gcgcggagcc aggtgccggt gaccgacgcg ggcaacctcg cccacaacgc
      181 ggtcacggcg ctcaacaccg ttaaggtagt tctcaatacc taccagcaga ttcagatcat
      241 gcagagtcag ctgcggtacc agctgcagtc cctcgagacc ctgaaccctt cttccttctc
      301 gggcctgctg gccgccataa atcagagcga gacgacctac gccatgctcc agggcgatct
      361 gacgacgatg ggcttcaccg tctctcagat caaccgcgac ttcgatcgtc tcttcccccg
      421 atctcagact cagtggaaca gcgtccgcta ttccgatttc aacgggtact acgaccggtg
      481 gaatggggag ctcgagacgt cggcgaaggc cgccgcgcgc gcccaggcgg gcatcagcgt
      541 cctcgatgca tcgaaccgtc agatcgccag cattcttgcg cagtccaaca cggcatccag
      601 cggtgaggtg cgccagctcc agctcatgaa ccagcaactc gcggtgcttc acgcagacct
      661 cgggtcgatc ctacagaacc tggccacgac ggggcgcgtt ctcacggact ggacggcggg
      721 gggggtcggc gagcagatgc tggcgcgcga gcgggctcgg cggcggctcg acggctacac
      781 cagccgcgga aagcccagcc gggttttgaa ggcgctgccg taagtcatgg atgtcaacgt
      841 cctcacgacc gtcctcacca cgttcgtcaa cgctctcgcg accggacttc attctgtcca
      901 atccggtgcc ggtgggatca tgcggtcgct ggccgcaatc gagatcgtcc tctgcggctt
      961 ctggatcgcg ctggatggcg gcgagcggct gtcgaccatt tgcaagttcc tactccagct
     1021 cacgttttgg atgttcttcg cagcgaactt cgccgcgctc gccaaggtct tttccgacac
     1081 gctgatccag tacggactgt ctgccagcgg tcagagtggc aacgtctcgc tgctcctcga
     1141 tccctcgcgc atcgccggaa tggccttcga tgcgacgcaa ccgctcattc agtcgatgga
     1201 cgacgccggc ctgacgcaca tgaaggacgc catcatcctc gggctctgct acctgctgat
     1261 catggcgtgc ttcttcgtga tcgcctgcca gatgtgcctc gccatcgtcg agtactacct
     1321 gatcgtcacg ttgtcgacct gcttggtgcc tttcggcatc tcgaaacaca cacggttcat
     1381 cgccgagaaa gctatcggcg cggtggtctc cgtcagcgtc aagctgatgg tcctcgggtt
     1441 catcgtcggt ctgatccaac cggttcttgc ccaactccga ttctctggcg gaggcgagat
     1501 caagctcaac gaggtcatgt ccatgatcct cgtgtgcggg ctcgtggccg tggtcgtctg
     1561 gcgcgcgccc gggttcgccg ccgaccttct cgcggcgtct ccgtccctct ccgccgctgc
     1621 ggtggggcag cacgtcaccg gggccgtctc gaacgcaaca cgagccgcgt cgggcgcttt
     1681 gtccgccggc gtcggcgcta ccagaaatgc cgccacgctc gtccgagcgg gagcgacggg
     1741 cacggccggc gcattccgca tggtcggctc cgcgatggcg cgtggatggc agggtagcac
     1801 cacggcggca gccgccggcg gcgatcggga gcttgcaggc gccttcggct cgcgtggcgc
     1861 gacgcagcca aaggcggccg ctcggacacc gcccccgcca acgaagtccg tgtgagcacg
     1921 gttttcgcgg ccgtgccgac aaggagggtg tgagacttcg accttccagt tgggagcccg
     1981 ctgcaccgct cgacacacct taccggcggg cgaggcaaga gtgggaccgc cgcatcggcg
     2041 gggccgtcgt gcaggcatcg aattggcgca gagccacttt cgcggtgctc gccgtggtag
     2101 cgctcgaggc cattggtctg gtcgtcctcg gtgcgcaacc gaaggcggtt ccgcatctca
     2161 tccaggtcga caagctcggc gccgcggcct acctcggccc gctcgagcgt gcgtcgctca
     2221 gggacttcca gccgccgccc gcgtcgttcg agtaccacct tcgccgcttc gtttcggaca
     2281 cccgcgagat ctcctcggac gtcgctgtcg ttcagcggaa ttggctcgac gcgtatcgcc
     2341 tcgtcaccca gaacggtgcc aaccagctga acgcctacgt gcgcgatcac gatccgatca
     2401 gtcggtccga gaacaaggcg cgcgtgtcgg tccaggtcga cgtcgtggtc cccatctccc
     2461 gcgagagctg gcaggtcgac tggaccgaga ccctctggga cgaacacggc ggtgagatcg
     2521 aaacctctgc gtggcgggcg acgtttcgca tcgtccttcg gatacctcag accgacgcag
     2581 agctgaagac caatccaatc ggcctctaca tcgacgaagt ccactgggcc cgacttgccg
     2641 acggaaggaa cgccaaatga tccgacgtct ctcactcttc gccgtctttg tgtctttcct
     2701 gaccggttgt gcgggccggc aacccgcgcc gcgctacgtg cgcgccgaac cggtgaagga
     2761 cgacccgccg aagccgaccg tcgtccaggt gccacatccc attcccgttc cgggtcagct
     2821 gaagcccgct cccgccacga accggcgccg ggccagctcg cccaaacacc cctccgacat
     2881 cattcgcgag gccaaccgca aggcgaccga agggccgtcg gcggacgact actacaacgc
     2941 gatcatgacc tacgactaca ccccggggac gctctaccag gtctacaccg ccccgcaaaa
     3001 gctcacggac gtccagctgc agtccgggga gcacatcgtg ggcaagccgg ccactggcga
     3061 caacatccgc tgggtcctcg cacgagggtc gtcggccgtc ggtccgaacg agcagcagca
     3121 catttacatc aagccgaccc ggccggacct cgacaccacg ctttgcatca acaccgaccg
     3181 tcgaagctac atccttgagc tgcactccta cgacgaagcg tggatggcgg cggtcgcctg
     3241 gcgctatccg caggatgaag tggcgcagct tgaaagcacc ctcgaccagc aacagatgct
     3301 cgcgcagtcg tcgacgccga ccggcgtcag tatcgacaag ctgaacttcg gctacggcat
     3361 cgccgtcctg tccggccgtc ctcaatgggt gccagttcag gtcttcgacg acacgcacaa
     3421 gacgtacatc cgcttccccg ctgcgatgct cgatcgcgaa gcaccggccc tcttcgtggt
     3481 ctcgagctcc ggtgacacgc agttggtgaa ctaccgagtc aagaacgaca cgtacatcgt
     3541 cgaccgactg ttcgaggacg cggaactccg cctcggccag cagacccagg aaatcgttcg
     3601 aatcgtccgg acccgatagg gcagtcatgg acgacaccac caagcgagac attcagccgt
     3661 cgtcgaacgc cgcacaatcg gcagacgaga tgacaggcag ccgtccggcc acaaatccga
     3721 gtgccggaca cgctgcaccc gggccggcag ccctaacggc accgagtcaa ttcacctatg
     3781 cttccaagct gccggctgat caccccggac tgcagctcga cgtccagaaa ggccggaccc
     3841 ttcggagggg cccggttgtc accggggtcg cgatcgtggc gggagcgacg gccatcgctc
     3901 tggtcgtggc cttttcgcca tcgaaagacg gtgcgaaggc agcgtcggcg acgccgtccg
     3961 cggagtcctc cccgccggaa atcgtcctac ctgactccat caaaaatccg acccccgctc
     4021 cgcggacgcc cgcgatgggg gcggcgcagc aggaccccac gcccgcgtcc ggcgcggcgg
     4081 gagggcgcca gcacctccgc gaggcgggga cgcggcggtc gggcgccggc gccgacgagc
     4141 gcaaccgaat tctcgcgtcg ccgatccttg tcgacgtcga caatcccgta cagacggaat
     4201 cgggcgtcca ccctcccgtt ctgggcgtgc cagccgccgg cctgttgtcc gcggatcgcg
     4261 ccgcggcgcc gccgccatcg agcgcatctg cgtccgtggc cggtcaaggc gatgccaaca
     4321 tgcagcagca caagagcgac tttcttactc gcgacggcct caacagttcc acgtacgtat
     4381 cgcagcctgt cctcgcgccg cggagcccgt acgaagtcaa agccggcacc atcatcccga
     4441 cgagcctcat caccgggatc aacagcgatc tgcccggcca gatcgtcggt caggtccgcg
     4501 agaacgtcta cgacacggtc acgggcaact atctgctcat cccacaaggc tcgcgcttgt
     4561 tggccaccta cgacagcgcc gtcacatttg gacagcagcg cgttctggtc tgctggaacc
     4621 gcctgattcg gccggacggc atctctattg gcctggagtg catgcctggc gtcgaccttt
     4681 ccggctacgc cggtttcgcg gatgacgtcg atcaccactg gtttcgaatc atcaccggcg
     4741 ttgcgctggg caccctcctc tccgcgactg ctcagcgaag ccagggcgac gtgtccgggt
     4801 acaaccccac cgtcccccaa ctttgggcgg cgaacgccgg gtccagcgtc aatcaggctg
     4861 gacaggcgat cacgcagaag aacctttcga tccagccgac catcaccgtc cgccccggct
     4921 actccgtcaa cgtgctcgtc agcaaggaca tcgtccttcc accctacaac cgagctgacc
     4981 gatgagccgt tggggctttc ccacgaggcg aagccagttc tgagcaccgt cccttccgga
     5041 cgccacccgc aacaggagat tgaccatgaa tcgtgaacac atcagccaag cagaactcgc
     5101 cggccggctc gggcagaagc ccgagctcga tcagaccagc ggtggagtga cattcgcccg
     5161 cctcaacatc gccacgtccg agcgctacac cgaccgatcg ggcgaaatcc gtgagaagac
     5221 cgagtggact cgcgcggtcg cctggggcga cctggccgag tcgatcgcga accggttcga
     5281 caagggttcc ccgatcgtcc tggcgggcgc gatgcgcgtc aacagctacg agaaggatgg
     5341 cgcgaagaac cgcgtcctcg agctgcacgt cgactcggcg gagcccaatc ttgaggccgg
     5401 cacttgtcgc aacgaggctc gcctggtcgg ggccgtgcgt agcgtcgaga gcaagacgct
     5461 cgacagcggg accgccatga ccgttctcag cgtcggcacc accacgaagc agaacggcaa
     5521 ggaccgagag gactggcaca gcgtcactct ttgggggcgg accgcggaga gcggtgccaa
     5581 gcagatcgcg gtcggcgatc tgatctccgt caacgggtcc atccgccacc gcagcgttcc
     5641 cggtgctgac ggtatcgagc gccgcctttc ggccatcgac ggtcgtcagt ttcaggtact
     5701 ggagcgcgcg aaagagcgcg accacgacca ggcggcgccg gccaagacac cggacctcgc
     5761 cagcgaggcc ggcggcgccg cgccccctcg tgcccggagc cgtcagcgcg gaaagagcgt
     5821 cgaacgcgac atgtagccgg ggcgccgggc gcggtccgcg tccggcgctt ctcctttcct
     5881 accggagccc accgatgaat agcgccgcac cgaacccgag gttgatcaga attctcctct
     5941 cggacgatga gctgctgtgg ctcgacatcg agctgaggcg tatcggcgag tggtcgaccg
     6001 atccgcctga cgtgcgggcg tacgccgggt tgaccaccaa agctgcgctc aagatctcca
     6061 agg
//
LOCUS       DASWVF010000612         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_221234, whole genome shotgun sequence.
ACCESSION   DASWVF010000612 DASWVF010000000
VERSION     DASWVF010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221234"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /gene="ybeY"
                     /locus_tag="VGD55_10765"
     CDS             <1..171
                     /gene="ybeY"
                     /locus_tag="VGD55_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0870869.1"
                     /translation="VQDELELLCTHGILHLLGFDHAEPDDEKEMFWLQRDLLTAWQSA
                     RTTQTSAESAGA"
     gene            168..1640
                     /locus_tag="VGD55_10770"
     CDS             168..1640
                     /locus_tag="VGD55_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0870870.1"
                     /translation="MNPGDVLWPICVVVLIFVAGLCAAADAALSRVSRVHVDDYLRDD
                     RRGAVALRRVIAEPPRYLNVVLLLRVAAEMTAAVMVAVWAIGRFGNSWETVLATAASM
                     VVVSYVVVGVAPRTLGRQHADGVALFAAPWVVRLAGILGPLPKFLIAVGNALTPGQGF
                     RDGPFTSEAELRDMVDLAEASKVIESDERQMIHSVFELGNTIVREVMVPRTDMVFIEQ
                     TKTLRQAMSLALRSGFSRLPVVGENEDDIVGTVYLRDITRRIFEYRESESTERVESLM
                     RPATFVPDSKPVDVLLREMQAERIHLVIVVDEYGGTAGLATIEDILEEIVGEITDEHD
                     REGPRIERLADGGVRVTARLPVDELAELFDVEIEDDDGVETVGGLLAQALGRVPIPGA
                     TATVAGLELVAEGARGRRNTIASVVVRLTDEERAKSRRRREAIDGIPGAEEPAAVPET
                     EALPMASSAAEAAGVAPASNGSGRTAAAAPSSDGSRRADV"
     gene            1633..>1890
                     /locus_tag="VGD55_10775"
     CDS             1633..>1890
                     /locus_tag="VGD55_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870871.1"
                     /translation="MSDPIELKPDDEKLVTLARSARARNNTVEGAAVRDDTGRTYVAT
                     TVELSSLPLSALQAAVVIAVASGADALEAAAVVGAAATARTE"
BASE COUNT          316 a          607 c          648 g          319 t
ORIGIN      
        1 gtacaagacg agctagaact gctttgtacg cacggcatct tgcacctgct cggcttcgac
       61 cacgccgagc cggatgacga gaaagagatg ttctggctgc agcgcgacct gctcaccgcg
      121 tggcagtcgg cccggacgac ccagacgagc gccgaaagtg ccggcgcatg aatcccggcg
      181 acgtcctgtg gccgatctgt gtggttgtcc tgatctttgt ggccggtctg tgcgccgcgg
      241 cggacgctgc gttgtcgcga gtctcgcggg tacatgtcga cgactatttg cgcgacgacc
      301 gacgcggcgc tgtcgcgttg cgaagggtga tcgcggagcc accgcgctac ctcaacgtgg
      361 tgctcttgtt gcgcgtcgca gccgagatga ctgccgccgt gatggtcgcc gtctgggcga
      421 tcggccggtt cggcaacagc tgggagaccg tgctggccac ggccgcgtcg atggttgtgg
      481 tcagctacgt cgtggtcggg gtggcgcctc gcacgcttgg ccgccagcac gctgacggtg
      541 tggcgctgtt cgccgcgccc tgggtcgtgc gtctcgccgg gattctcggg ccgctgccga
      601 agttcctcat cgcagtcggc aatgcgctga cccccggtca aggttttcgc gacgggccgt
      661 tcaccagcga agcagagctg cgcgacatgg tcgacctcgc cgaggccagc aaggtgatcg
      721 agagcgatga gcgccagatg atccactcgg tcttcgagct gggcaacacg atcgtgcgcg
      781 aggtgatggt gcctcgcacc gacatggtgt tcatcgaaca gaccaaaacg ttgcgacaag
      841 ccatgtcact cgcgttgcgc agcggattct cccggttgcc ggtggtcggc gagaacgaag
      901 acgacatcgt cggcaccgtc tacttgcgcg acatcacccg ccgcatcttc gaataccgtg
      961 aatctgagtc gaccgagcgc gtcgaatcgc tcatgcggcc ggcgacgttc gtaccagaca
     1021 gtaagccggt cgacgtactg ctgcgcgaga tgcaggcgga gcgcattcac ctcgtcatcg
     1081 tcgtcgacga gtacggcggc accgcgggcc tcgccacgat cgaggacatt cttgaagaga
     1141 tcgtgggcga gattactgac gaacacgatc gcgaaggccc gcgcatcgag cgactcgccg
     1201 atggtggtgt tcgggtcact gcccggctgc cggtcgacga actcgcggag ctgtttgacg
     1261 tcgagatcga agacgatgac ggtgtcgaga ccgtgggcgg cctgctcgcg caggcacttg
     1321 gccgagtacc gattcccggc gcgaccgcaa cggtcgccgg ccttgagctg gtcgccgagg
     1381 gcgcacgcgg tcggcgcaac acgatcgcca gcgtcgtcgt ccggctgacc gatgaagaac
     1441 gcgccaagtc gcgtcgaagg cgcgaggcga ttgacgggat ccctggtgcg gaagagcctg
     1501 ccgccgtgcc cgaaaccgag gcgctcccca tggccagcag cgccgctgaa gcggccggtg
     1561 tggcaccggc gtccaacgga tcgggtcgca ccgccgccgc agcaccgtcg tccgatggat
     1621 cgcggcgcgc cgatgtctga cccgatcgaa ctcaagccgg acgacgagaa gctggtcact
     1681 ctcgctagat cagcacgagc gcgcaacaac acggtcgagg gtgctgctgt gcgcgacgac
     1741 accggccgca cttacgtcgc gaccaccgtc gaactgtcgt cgcttccgct ttcggcgcta
     1801 caggcggccg tcgtcatcgc ggtggcgagt ggcgcagacg cgctcgaagc ggccgcggtc
     1861 gtcggtgccg cagcgacggc tcgcaccgag
//
LOCUS       DASWVF010000613         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_221253, whole genome shotgun sequence.
ACCESSION   DASWVF010000613 DASWVF010000000
VERSION     DASWVF010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221253"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGD55_10780"
     CDS             complement(<1..332)
                     /locus_tag="VGD55_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870872.1"
                     /translation="MTRRRLQAVLGYENRAGPHGAVPVCVAAACAISLSVAGLLPTIV
                     IVGAGAAVALAHRRRSRAQQVTETQIAVVDLCRATAAELRAGHASARAFASAADAAPL
                     RLAVLLRTA"
     gene            complement(349..1509)
                     /locus_tag="VGD55_10785"
     CDS             complement(349..1509)
                     /locus_tag="VGD55_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEY0870873.1"
                     /translation="MTADAALVARVRGRLADDASAPTAARVAALVREEAGVRGTAQVL
                     AAIETLRSELLGAGPLEALLHETGVTDVLVNGPREIWVDRGSGLELADLHFSDEREVR
                     RLAVRLAAGVGRRLDDAVPCADIRLPDGVRLHAVLPPVSPSGAHLSFRVPRQRAFSLD
                     EMVDGETMSLLAAKLLRDVIASRASFVVTGGTGTGKTTLLSTLLGCVDPRERIVVVED
                     SGELRPDHPHVVRLEARSANIEGVGAIELRSLVRHALRMRPDRLVVGEVRGAECVDLL
                     AALNVGQDGGAGTLHANSVADVPARIEALCTAAGLTREAAHSQLAAGVQVVVQLARRA
                     DGRRVLDSIGVVVRRSDGLADVLPAVRVRGSSWFDDEGRAALDHVLQRRAEL"
     gene            complement(1506..2585)
                     /gene="ssd"
                     /locus_tag="VGD55_10790"
     CDS             complement(1506..2585)
                     /gene="ssd"
                     /locus_tag="VGD55_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEY0870874.1"
                     /translation="MTADPDLLDDVLRLCAAAGVDVEVAPDSTAARPSWARASLVLVG
                     DDVSVAINRAALRHRADVVLVSRDLDHGNIWERACGVGAEHVAVLPDAEPWLVSRLAD
                     AVDGGGSRGLVVGVVGGRGGAGASTLAAAMAVTAMRAGRRAMLVDADPYGGGADLLFG
                     GEHALGLRWPDVASAKGRIGGEELVNSLPRMGELAVLSCDRHDPPELKAEAMSSVLEA
                     AVRACDMVIVDLPRRIDGAAQVALAAANVVLLLVPAEVRAAAAAARVASEVARFADDV
                     RVVVRGPAPGGLDAVDIASALGLPLAGQLKAEPDLALALERGEVPAGRGRGPLAEFCR
                     GFLAPTLSDAHAVAPIGAEPAWLDR"
     gene            2979..3785
                     /locus_tag="VGD55_10795"
     CDS             2979..3785
                     /locus_tag="VGD55_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY0870875.1"
                     /translation="MSTTRPAAFFDLDKTIIAKSSVMAFGRPFYQGGLINRRALLKST
                     YAQFVYLVGGADHDQIERMRSYLSQLCKGWDVEQVKTIVAETLHDLIDPLIYDEAAAL
                     IEEHHAAGLDVVIVSTSGIEVVEPIGELLGADRIIATRMVVKDGKFTGDIDYYAYGEA
                     KAAAVTELAAEEGYDLSASFAYSDSSTDIPLLELVGFPSVVNPDRALRKEAMSRGWPV
                     LNFSNPVPLRARVGLGSPRSPVYAAAGLGAGMAAAGLVWYARRRNKAASA"
BASE COUNT          789 a         1512 c         1358 g          583 t
ORIGIN      
        1 gctgtccgta agagcacggc caaccgcaac ggcgctgcat ctgcggccga cgcgaatgcc
       61 cgggccgacg catggccggc gcgcagttcg gctgcggttg cgcggcacag atcgacgacg
      121 gcgatctgcg tttcggtgac ctgctgcgcg cggctgcgac gacgatgtgc cagcgcgaca
      181 gctgcgccgg cacccacgat cacgatcgtg ggaagcagcc cagcgactga gagcgagatc
      241 gcacacgcgg cagcgacgca caccggaacc gcgccgtgcg ggccggcgcg gttctcatag
      301 ccgagcacgg cctgcaaacg gcgccgcgtc acaggcccac ctgtggtgtc acaactcggc
      361 ccgtcgttgc aacacgtgat cgagcgcagc gcgaccctcg tcgtcgaacc aactcgaacc
      421 gcgcacacgc actgcgggca acacatcggc aagaccatca ctgcgccgga cgacgacacc
      481 gatcgagtcg agcacgcgtc gcccgtctgc ccgccgggcg agctgaacaa ccacttgcac
      541 gccggcggcg agttgactat gcgccgcctc gcgcgtcaac ccggctgcag tgcacagcgc
      601 ctcgatgcga gctgggacgt cggcaactga gttcgcatgc aaggtgccag cgcccccgtc
      661 ttgaccgacg ttcaatgccg cgagcaggtc gacgcattcg gcgccgcgca cctccccgac
      721 aacgaggcgg tcgggtcgca tccgaagggc gtgccgcacc aatgaccgca gctcgatagc
      781 gccgacgcct tcgatgttcg ccgaccgcgc ctcgagcctt acgacgtgcg gatgatcggg
      841 acgcagctcc cctgaatcct cgacgacgac gatgcgttca cgtggatcga cgcaaccgag
      901 caacgtcgac aagagcgtcg tcttgccggt gcccgtacca ccggtcacca caaacgaggc
      961 ccgcgacgca atcacatcgc gcaagagctt ggctgcgaga agcgacatcg tttcgccgtc
     1021 gaccatctcg tctagggaga acgcacgctg ccgggggacg cggaacgaga gatgtgcacc
     1081 gctgggcgac accggcggca acactgcatg cagccgcacc ccgtcgggaa gccggatgtc
     1141 tgcacagggc actgcgtcgt cgagacgcct gccaaccccc gctgcgagtc gaaccgccaa
     1201 tcggcgaacc tcgcgctcgt cgctgaaatg caggtcggca agctccagcc cgctaccgcg
     1261 gtcgacccag atctcgcgcg gcccgttgac gaggacgtcg gtcactccgg tctcgtgcaa
     1321 gagtgcctcg agcggccccg caccgagcaa ctcgctacgc aacgtttcga tggcggcgag
     1381 aacttgcgcg gtgcctcgca cgccggcctc ttcgcgcacg agcgccgcta cccgcgctgc
     1441 cgtcggtgcg ctcgcgtcgt ccgcaagccg accgcgcacc ctcgccacga gcgcggcgtc
     1501 ggccgtcacc ggtcgagcca cgcgggttcg gcgccgatgg gcgcgaccgc atgcgcgtcg
     1561 gagagagtcg gggccagaaa cccccggcag aactcggcga gcggcccgcg gccccgaccg
     1621 gccggaacct caccgcgttc aagagccaag gcgaggtcgg gctcggcctt gagttgcccc
     1681 gccaacggca gacccaacgc gctggcgatg tcgacagcgt cgagaccacc cggggcaggg
     1741 ccgcgaacga cgacccgaac atcgtcggcg aacctggcaa cctcgctcgc gacccgtgcc
     1801 gccgccgcgg ccgcacggac ttccgccggc accaaaagga gcacgacgtt cgcagcggcc
     1861 agggcgacct gtgccgcgcc gtcgatgcgg cgcggcaggt cgacgatcac catgtcgcac
     1921 gcccggaccg ctgcctcgag cacgctcgac atcgcctcgg ccttcagctc gggcggatcg
     1981 tgacgatcgc acgacagcac cgcgagctcg cccatgcggg gaagtgagtt gacgagttct
     2041 tcaccaccga tgcgaccctt tgcactcgcg acatcgggcc accgcaagcc gagtgcgtgc
     2101 tcgccgccga acagtaggtc ggcgccgccg ccatagggat cggcatcgac caacatggca
     2161 cggcggcctg cccgcatcgc ggtgaccgcc atcgctgcgg cgagcgtgct cgcacccgcg
     2221 ccgcctcggc caccgacgac gccgaccacc aacccacgcg agccaccacc atcgaccgcg
     2281 tcggccagtc gagagacaag ccagggttcg gcgtcgggca gcacggcgac gtgttcggca
     2341 ccgacgccgc aggcgcgctc ccagatgttg ccgtgatcaa gatctcggct gaccaagacg
     2401 acatcggcgc ggtgtcgcaa cgccgcccgg ttgatcgcca cgctgacgtc gtcaccaacc
     2461 aacacaagcg atgcccgcgc ccatgaagga cgcgccgccg tggaatcggg agccacctcg
     2521 acatcgaccc ccgcagccgc gcacagccgc aggacgtcgt cgagaaggtc cgggtcggca
     2581 gtcacgagca gtggacggtt gccgcctaca gggatgggca tgcattgacg atgtggggcg
     2641 atgacccaca cagccacgcg ccggaccgcg gctgtggatc accgatcgaa tccgagcccc
     2701 tggggacaac cgaacccaga tcgaaacggg caaccgaagc ggacgcccga acgttgggaa
     2761 ttaaggaacg gcccccgccg ggggggtagc gggggccgtt cacgaagggt ccgactctgg
     2821 ggggagagcc gtaccagtac aaacgtaacg cttctacacc gctcggcaca cctccccaca
     2881 gcgactttct atgacgaaca gaacgtcggg acaaaagacc tatgcacaca ccgccactac
     2941 cggacggacg gccaccttcg gcgtcctatt ctcaagtcat gagcaccacg cgaccggcag
     3001 cctttttcga cctcgacaag acgatcatcg ccaagtcgag cgtcatggct ttcgggcgcc
     3061 cgttttacca gggcgggctc atcaaccggc gcgccctgct caagagcacc tacgcccagt
     3121 tcgtctatct cgtgggcggc gccgaccacg atcagatcga acgcatgcgg agctatcttt
     3181 cccagctgtg taagggatgg gacgtcgaac aggtcaagac gatcgtcgcc gaaacgctcc
     3241 acgacctgat cgacccgctg atctacgacg aggccgccgc gctcatcgaa gagcaccacg
     3301 cagccggcct tgatgtagtc atcgtgagca cctcggggat cgaggtggtc gaacccatcg
     3361 gtgagctgct cggcgccgac cgcatcatcg ccacccgcat ggtcgtcaaa gacggaaagt
     3421 tcaccggcga catcgactac tacgcctacg gcgaagccaa ggcggccgcc gtcaccgaac
     3481 tcgctgccga agagggctac gacctctcgg cgtcgttcgc ctacagcgac tcctcgaccg
     3541 acatccccct gctcgagctc gtcggcttcc cctcggtcgt caacccggat cgcgccctgc
     3601 gcaaggaggc gatgtccagg ggttggccag tgctcaactt cagcaatccg gtgccactgc
     3661 gggcgcgcgt gggtcttggt tcgccgcgca gcccggtcta tgcggcggca ggcctgggcg
     3721 ccggcatggc cgccgcgggc ctggtctggt acgcccggcg gcgcaacaag gccgcgtcgg
     3781 catagcggcc cggcacagag ctcacgcaac cgcgtcattt cccagcgaca gccggggcgg
     3841 ccaccggggt accaaagact gagagtcttc cctgtcgcag cctcttccga tcgagtggcg
     3901 ataggcgtag aaacaaattg ggtcgggtac ggcgaggggt cacccctcga agttccgggt
     3961 ccgcgcacga cacctcaggc acccacgcgg aaccagccga atcaggcggt cgagtcgggc
     4021 ggtgactcct cggagtttcc tgctcgccga cacttcgcag gtgcacgctt ggtaaccgga
     4081 ggatcgtgcg agcagcggcg tcccgtgcaa acggggcgcc gctcgcattt gctgatcgtg
     4141 gacgtaaagc gacggtcaag ccagccgagc tcgcggttct ttgaggcgtc gacgggtact
     4201 tgatcggacg ccgaggcggt gccaacttgg tgcggtacga at
//
LOCUS       DASWVF010000614         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_501298, whole genome shotgun sequence.
ACCESSION   DASWVF010000614 DASWVF010000000
VERSION     DASWVF010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501298"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VGD55_10800"
     CDS             complement(<1..999)
                     /locus_tag="VGD55_10800"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY0870876.1"
                     /translation="MPAIDRHRLATLIQRERVAYAVAHPASKALNQSATHLLGRVPMT
                     WMAKWSGGFPLYLDQARGNRVVDADGHEYVDFALGDTGAMAGHSPQATVDAVADRIDR
                     LGGITAMLPTADAEWVAADLTRRFGLPHWSFTLTATDANRWAIRLARLVTGRPKVLVF
                     AYCYHGSVDEAFALAGPDGEAVPRPGNVGPPVPLHLTTRVAEFNDLASVESALAHGDV
                     AAILTEPALTNIGIVLPEPGFMAGLRALATRYGALLMIDETHTISAGPGGATRLWDLE
                     PDIFVIGKSIGGGIPTGAYGVSTEVLRMVEAHQEADLVDVGGVGGTLAGNALSIAAM"
     gene            complement(1004..2095)
                     /locus_tag="VGD55_10805"
     CDS             complement(1004..2095)
                     /locus_tag="VGD55_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870877.1"
                     /translation="MTASSAGSVGVGSTDAASAGAVSTSPTQLPAASGSNVLPLPSAS
                     GPLSIFADVVIKGAFSTAQAGLTAAEGPDGAVFVAGVATSPQIVWVVDGISPAAVAEH
                     LAGPVTALAADADNLYVGVGKSVYAYSRTTGALVHTWTQAALFGTVNQLALAGNRLWG
                     LYTAPDDGPPVAPAALVEIDPSSNHPVRTVTGITGAFSIAAGSSGVYYVTKQSSQLVE
                     QTNNGDTVSVPTHQSVDLQLSGPSAIQAVAVVGGQVVLQHDAGQGLDAVLNTYDAGSL
                     AGPSAPISFSAAEQLVATPSGLFVIGNPDTGACSAGQQTCVRRFSLAGGNVGDPLALP
                     DDTLATDVTGPYPTVVLGAGSDVHVLRIG"
     gene            2439..>3903
                     /gene="kaiC"
                     /locus_tag="VGD55_10810"
     CDS             2439..>3903
                     /gene="kaiC"
                     /locus_tag="VGD55_10810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEY0870878.1"
                     /translation="MTAQAAVALKAPVPFPERGLRKTATGISGLDEVTGGGLPFGRPT
                     LVCGPAGCGKTLLAMEFLVRGIIEFDEPGVFVSFEETVDELVTNVVSLGFDLQQFQDD
                     GLLVVDQIYLAPEDVQVSGAWDLQGLFIRLGAAIDEVGAKRVVLDTIETLFGAFADAA
                     VLRAELRRLFAWLKQKGVTAVITGERGDGTLTRHGIEEYVSDCVIVLDHRVNEQTSTR
                     RLRVLKYRGSLHGTNEYPFLIGANGISVLPITSLNLQHTVSDKRVSTGMESLDAMLDG
                     GGVYRGSSVLISGTAGTGKSTMAAQFCAAACGRGERALYFAFEESQAQIVRNMASVGI
                     DLAHWVNEGLLQFRCIRPSLLGLEAHLASMQEIVSAFDPAVVVMDPISDIFHAGEGDE
                     VAAMLTRQVDYLKSRGVTAVFTSLNTIRDAENSEQQIGSLIDTWVIAKLAEFDGGRTR
                     VLYVLKSRGMAHSHEIRDFVLSETGLELTGIYHGARAG"
BASE COUNT          675 a         1258 c         1328 g          642 t
ORIGIN      
        1 catcgcggcg atcgagagtg cgttgcccgc gagagtgcca ccgacgccgc cgacgtcgac
       61 gaggtcggcc tcctgatggg cctcgaccat gcgcaagacc tcggtgctca cgccataggc
      121 gccggtcggt atgccgccgc cgatgctctt gccgatcacg aagatgtcgg gctccagatc
      181 ccagagccgg gtagcaccac ccgggccggc cgagatcgta tgcgtttcgt caatcatcag
      241 cagggcgcca tagcgcgtcg ctagtgcgcg aaggcccgcc atgaaccccg gctccggcag
      301 cacgatgccg atgttcgtca gcgccggctc ggtcaaaatc gcggccacgt caccgtgcgc
      361 cagcgccgac tcgacgctgg cgaggtcgtt gaactccgcc actcttgtcg tcaggtgcag
      421 gggcacgggt ggtccgacgt tgccgggtcg cggcaccgct tcgccgtcag gcccggcaag
      481 cgcgaacgct tcgtcgaccg acccgtggta gcagtaggcg aacaccagca ccttcggacg
      541 cccggtcacg agccgcgcca acctgatcgc ccagcggttg gcgtctgtgg ccgtcagcgt
      601 gaaggaccaa tgcggcagcc cgaaccgccg ggtgaggtcg gcggccaccc actcggcgtc
      661 cgcggtggga agcatcgccg tgatcccacc caagcggtcg atgcgatctg cgactgcgtc
      721 gaccgtcgcc tgcgggctgt gccccgccat ggcgccggtg tcaccgagag cgaagtcgac
      781 atattcgtga ccgtccgcgt cgacgacccg attccctcgt gcctggtcca gatagagcgg
      841 gaatcccccg ctccacttgg ccatccacgt catcggcacc cgaccgagca gatgcgtcgc
      901 ggactggttc agcgctttgg acgccgggtg cgccacggcg taggcgaccc gctcgcgctg
      961 gatgagggtc gcgagtctgt gccggtcgat ggcaggcatg cggtcagccg atgcggagca
     1021 cgtgcacgtc ggatcctgct ccgaggacca ccgtcggata ggggccggtc acgtcggttg
     1081 ccagcgtgtc atcgggcaac gcgagcgggt cacccacgtt gccgccggcc aacgagaacc
     1141 ggcgcacgca cgtctgctgc ccagcactac acgctccagt atcggggttg ccgatgacga
     1201 acaacccgct tggggtggcc accaactgct cggcggccga gaaagagatc ggggctgacg
     1261 gtccggcaag tgaacccgcg tcgtaggtgt tgagcaccgc gtcgaggccc tgacccgcgt
     1321 cgtgctgcaa cacgacctgc ccgcccacaa ccgcgaccgc ctggatcgcc gacggacccg
     1381 acaactgaag gtcgacggat tggtgcgtcg gcacagacac cgtgtcgcca ttgttggttt
     1441 gctcgaccag ctggctgctc tgcttggtca cgtagtacac gcccgaactg cccgcggcaa
     1501 tggagaacgc gccggtgatg ccggtgacag tgcgcaccgg atggttcgac gaggggtcga
     1561 tctcgacgag agcagcaggt gccactggcg gcccgtcgtc cggcgccgtg tacaggcccc
     1621 acagccgatt cccggcaaga gcgagctgat tcaccgtgcc gaacaacgcg gcctgcgtcc
     1681 acgtgtggac cagcgcgccc gtcgtccggc tataggcata caccgacttt ccgacgccga
     1741 cgtagaggtt gtcggcgtcg gcggcgagcg cggtgacagg cccggccagg tgctccgcta
     1801 cggctgccgg gctgatgccg tcgacgaccc agacgatctg cggtgacgtc gccactcccg
     1861 ccacaaatac cgcgccgtcg gggccttcgg cggctgtcag gccggcttgc gcggtggaga
     1921 acgcaccctt gatcaccaca tcggcgaaga tcgacagcgg cccgctcgct gagggcaagg
     1981 gcagcacatt cgagccggac gccgctggca gctgagtcgg ggacgttgag accgcacccg
     2041 ccgaggccgc atccgtggag ccgaccccga ccgaaccggc cgaactcgca gtcacggccg
     2101 ccgacgaagc cgccgctggg cgggggctgg ccgacggacc acttccctgc ggcgccgggg
     2161 cccgatgggt ggaagtgcag gccacgagca accccgcggc gagacatcgg gaagcgatga
     2221 gccgcaacgc tgcggcgtcc aggccacgcc gctcagtcga tctgttggtt tcgataggca
     2281 cagatcctgc cacggtgaga gccgaatgat gatcgccgca agcgcaagag tcgttgtggc
     2341 gatgtgggcg ggaggaacct gtttcgactg gtttgtgaag tgtcactgtg ggtagcaaat
     2401 cagctacagg ccgaccctcg gtcaggttgg agacgcccat gaccgcacaa gccgcggtgg
     2461 ccctgaaagc acccgtcccg ttccccgaga ggggcctgcg caagaccgcc acgggcatca
     2521 gtgggttgga cgaggtgacc ggtggagggc tgccgtttgg tcgtccgacg ttggtctgcg
     2581 gaccggcagg gtgcggcaag acgctgctcg ccatggagtt tctggtgcgc ggcatcatcg
     2641 aattcgatga accgggagtg ttcgtctcgt tcgaggaaac ggtcgacgaa ctcgtcacca
     2701 acgtggtgtc gctcggcttc gacctgcagc aatttcagga cgacgggctg ctggtggtcg
     2761 accagatcta cctggcaccg gaggacgtgc aagtttccgg tgcgtgggac ctgcagggat
     2821 tgttcatccg gcttggagcc gcgatcgacg aggtcggcgc gaagcgggtg gtgctcgaca
     2881 ccatcgagac gttgttcggc gcgttcgccg acgcagctgt tttgcgggcg gagttgcgcc
     2941 ggcttttcgc ttggttgaag cagaagggtg tcaccgcagt tatcaccggc gagcgcggcg
     3001 acggcacgct cacgcggcac ggcatcgagg agtacgtatc ggattgcgtg atcgtcctcg
     3061 accaccgggt gaatgagcag acctcaacaa ggcgcctccg ggtactgaag taccgcggct
     3121 cgctgcacgg cacgaacgag tacccgttcc tgatcggcgc taacggcatc tcggtcttgc
     3181 ccatcacctc gttgaacctg cagcacacgg tgtctgacaa acgggtgtcg accggaatgg
     3241 agagcttgga cgcgatgctg gacggtggcg gcgtctaccg gggcagctcc gtgctgataa
     3301 gcggtactgc gggcaccggg aaatcgacga tggcggcaca gttctgcgcg gcagcttgtg
     3361 gccgcggcga acgcgcgctg tacttcgcct tcgaggaatc acaggcgcaa atcgtgcgca
     3421 acatggcatc ggtgggcatc gacctggcgc actgggtcaa tgagggattg ctccagttcc
     3481 ggtgcatccg gccgagcctg ctgggattgg aagcacacct ggcgtcgatg caggagatcg
     3541 tctcggcatt cgaccccgct gtggtcgtga tggatccgat ctctgacatt ttccacgctg
     3601 gcgagggcga cgaagtcgcc gcgatgttga cgcgccaggt cgactacctg aagagccgcg
     3661 gcgtgaccgc cgtgttcacg tcgttgaaca cgatccgcga cgccgagaac tcagagcagc
     3721 aaattggatc cttgatcgac acctgggtga ttgcaaaact cgccgagttc gacggcggcc
     3781 gtacccgcgt gttgtatgtg ctgaagtcgc gtggcatggc ccactcgcac gagatccgcg
     3841 acttcgtact gagcgagacc ggcctcgagc tcaccggcat ttaccacggg gcccgagcgg
     3901 gtc
//
LOCUS       DASWVF010000615         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_348347, whole genome shotgun sequence.
ACCESSION   DASWVF010000615 DASWVF010000000
VERSION     DASWVF010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348347"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGD55_10815"
     CDS             <1..341
                     /locus_tag="VGD55_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0870879.1"
                     /translation="YGSGGSSSGVVRILKDLDHGIDGLNVLVVEDIVDSGLTLSWLVA
                     NLRSRGPASVEVCALMRKPDAVKQDVDVRYLGFDIPRDFVVGYGLDYAEKYRNLPFVA
                     TLKRSVYDPQ"
     gene            549..>1726
                     /locus_tag="VGD55_10820"
     CDS             549..>1726
                     /locus_tag="VGD55_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY0870880.1"
                     /translation="MNVKRYARGPLFWVLLIVVFLVLLNGLWSSGSSFKSVSTSQIVS
                     NISSGNVSTAVFHDKEQTVQLTLKTPLAGTQTTKIQASIAPDQELQLENQLDAVATAQ
                     KLDRSKLYTVKVSHTSLLLEILLNALPILLLVGLMIFMLTQMQGGGSRVMNFGKSKAK
                     LISKDTPKTTFADVAGADEAIEELVEIKEFLENPAKFQAIGAKIPKGVLLYGPPGTGK
                     TLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFTQAKENAPAIIFIDEIDAV
                     GRHRGAGLGGGHDEREQTLNQLLVEMDGFDVKGGVILIAATNRPDILDPALLRPGRFD
                     RQIVVDRPDMEGRKGILRVHAKGKPFEGDVDLDVIARRTPGFTGADLANVINEAALLT
                     A"
BASE COUNT          300 a          536 c          564 g          326 t
ORIGIN      
        1 cctacggctc cggcggctcg tcgtctgggg tggtccggat tctgaaggac ctcgaccacg
       61 gcatcgacgg cctgaacgtg ctggtcgtcg aggacatcgt cgactcaggg ctcacgctct
      121 cgtggttggt ggctaacctg cgctcgcggg gtccggcttc ggtcgaggtg tgcgcgctga
      181 tgcgcaagcc agatgccgtc aagcaggacg tcgatgtgcg ctatctcggc ttcgacattc
      241 cccgtgactt tgttgttggg tacgggctcg actatgccga gaagtaccgg aacctgccgt
      301 tcgtcgcgac ccttaagcgt tccgtatacg accctcaata aaggtgggca cgacccttcg
      361 taaggtgtgg ggccgaccct caataggttc ggttcgggaa cgaccgaggg gggctggggg
      421 ttatgcagtg ggcggcgggg gtttcggctg tgggtgaacg cttagcccgc ggtgtaccgt
      481 cgagggtggt acgagatgtc ggtagcgcgt cgattcggta ggagggacgg agctttcgct
      541 ccgcatccat gaacgtgaaa cggtatgccc ggggcccgct gttctgggtc ctcctcattg
      601 tcgtgttcct cgttctgctg aacgggctct ggagcagcgg cagctccttc aagtctgtgt
      661 cgacgtcgca gatcgtgtcg aacatctcct ccggcaacgt ttctacggcg gtgttccacg
      721 acaaggaaca gaccgtccag ctcaccttga agacaccgct tgccggcacg cagaccacga
      781 agatccaggc ctcgatcgcg cccgaccagg agctccagct ggagaaccag ctcgacgccg
      841 tggcaaccgc gcagaagctc gatcgctcga agctgtacac ggtcaaggtc agccacacga
      901 gcttgctgct cgagatcttg ctgaacgcgc ttccgatcct gctgctggtc ggcctgatga
      961 tcttcatgct gacccagatg cagggcggcg gctcgcgggt catgaacttc ggcaagtcca
     1021 aggcgaagct gatcagcaag gacaccccaa agaccacctt cgccgacgtc gcgggggccg
     1081 acgaggcgat cgaagagctg gtcgagatca aggagttcct cgagaacccg gccaagttcc
     1141 aggcgatcgg cgcgaagatt cccaagggcg tcctgctcta cggccctccc ggtaccggca
     1201 agaccttgct ggcgcgggcc gtggctggcg aggccggcgt cccgttctat tcgatttcag
     1261 gctctgactt cgtcgagatg ttcgtcggcg tcggagcgtc gcgggtgcgc gatctcttca
     1321 cccaggcgaa ggagaacgct ccggcgatca tcttcatcga cgagatcgac gctgtcggcc
     1381 ggcaccgcgg tgccggactt ggcggcggcc acgacgagcg tgagcagaca ctgaaccagc
     1441 tcctcgtcga gatggacggc ttcgacgtga agggcggcgt gattttgatc gccgcgacca
     1501 accgtcctga catcctcgac ccagcgctgc tgcgtcctgg ccggttcgac cgtcagatcg
     1561 tcgtcgaccg gcccgacatg gaaggccgca aaggcatcct tcgggtgcac gccaagggca
     1621 agccgttcga gggcgacgtc gacctcgacg tgatcgctcg gcgcacccca ggattcaccg
     1681 gcgccgacct cgcgaacgtg atcaacgagg cggccctgct taccgc
//
LOCUS       DASWVF010000616         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_322584, whole genome shotgun sequence.
ACCESSION   DASWVF010000616 DASWVF010000000
VERSION     DASWVF010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322584"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2489)
                     /locus_tag="VGD55_10825"
     CDS             complement(<1..>2489)
                     /locus_tag="VGD55_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0870881.1"
                     /translation="GFSAVTKAWFNSAFATPTPAQVLAWEAIGRGENTLVVAPTGSGK
                     TLAAFLWSLDRITAAPVPADPSKRCRVLYISPLKALAVDVERNLRAPLVGMRHAATRL
                     GLPEPKVEVALRSGDTPAVERRRLTTKPPDILITTPESLFLILTSAARESLQAIDTVI
                     VDEVHAVAATKRGAHLALSLERLDAMLEKPAQRIGLSATVRPVDEIARFLSGPRPATV
                     VQPPAVKTFDISVVVPVEDMDSPDEATDEEPRKSIWPHVEERISDLIDAHRSTIVFAN
                     SRGLAERLTHRLNDIGFERGRETEIARAHHGSVSREQRSITEEMLKSGQLPAVVATST
                     LELGIDMGAVDLVVQVESPPSVASGLQRIGRAGHQVGAVSRGVIFPKYRGDLVQTAVV
                     AERMKAGAIESIRIPRNPLDVLAQHLVSMTAMQTWDVDDALALVRRAAPFAALPQSAF
                     DAVLDMLAGRYPSDEFAELRPRLVWDRVAGTIVGRPGSQRLAVTSGGTIPDRGLFGVF
                     LAGEGNSRRVGELDEEMVYESRVGEVFTLGSSSWRIVEITHDQVLVVPAPGVPGKLPF
                     WHGDAQGRPLELGRALGAFVRELAALPKAAAHARLLDAGLDDWAAANLLSYLAEQAQA
                     TGRLPDDHTIVVERFHDELGDWRVVVHSPFGAQVHAPWALAIGARLRERYGVDVQTMH
                     ADDGIVLRIPETDEAPPAQLALLASDEVDDAVTREVGGSALFAARFRECAARALLLPR
                     RRPDRRTPLWQQRQRSAQLLSVASEYGSFPIVLETMRECLQDVFDVPGLVELMRDIEA
                     RRVQLVDVETPAASPFARSLLFGYVAAFIYE"
BASE COUNT          424 a          802 c          857 g          406 t
ORIGIN      
        1 cctcgtagat gaatgcggca acgtagccga agagcagcga acgtgcgaac ggcgatgctg
       61 cgggcgtctc gacgtcgacg agctgtacgc gtcgcgcctc gatgtcgcgc atcaactcaa
      121 cgagcccggg aacgtcgaac acgtcttgca ggcactcgcg catcgtctcg agcacgatcg
      181 ggaacgagcc gtactcactg gcgaccgaca acagttgcgc cgagcgttga cgctgttgcc
      241 acagcggtgt tcgacggtcg ggacgccgac ggggaagcag caacgcacgc gcggcacact
      301 cccggaaccg tgcagcgaac aatgccgagc cgcccacttc gcgggtgacc gcatcgtcga
      361 cctcgtcgga ggccagtagc gcgagttgcg cgggcggggc ttcgtcggtt tcggggatgc
      421 gcagcacaat gccgtcgtcg gcgtgcatgg tctggacgtc gaccccgtag cgttcgcgca
      481 atcgcgcgcc gatcgcgagc gcccagggtg catgcacctg cgcgccaaag ggggagtgca
      541 cgacgacccg ccagtcgccg agttcgtcgt ggaatcgctc gaccacgatc gtgtggtcgt
      601 cgggcagccg gccggtggct tgcgcctgct cggctaggta cgacagcagg ttcgctgccg
      661 cccagtcatc gagacctgcg tcaagcaacc gcgcatgtgc tgcggctttc ggcaacgccg
      721 cgagttcgcg aacgaacgcg ccgagcgcgc ggccgagttc gagcggtcga ccctgggcgt
      781 cgccatgcca gaacggcagc ttgcccggta cgccaggtgc agggacgacg agcacctggt
      841 cgtgcgtgat ctcgacgatg cgccacgacg acgaaccgag cgtgaacacc tcaccgacgc
      901 gtgactcgta gaccatctcc tcgtcgagct cgccgactcg tcgcgaattt ccctcgcctg
      961 caaggaaaac tccgaaaaga ccgcggtcgg gaatcgtgcc accgctggtg acagcgagcc
     1021 gctgcgagcc tggccgaccg acgatggtgc cggcaacccg gtcccacacg agtcgagggc
     1081 gcagctcggc gaattcgtcg cttgggtacc gacccgcgag catgtcgagc accgcatcaa
     1141 acgccgattg ggggagtgcg gcgaagggcg ctgcgcgccg aaccagtgcg agggcatcgt
     1201 cgacgtccca tgtctgcatt gcggtcatcg agacgaggtg ctgcgcgagc acatcgagcg
     1261 gattgcgcgg gatgcgaatc gactcgatcg cgcccgcttt catgcgttcg gccaccaccg
     1321 ccgtctggac gaggtcgccc ctgtacttgg ggaagatcac gccacgcgag accgcgccga
     1381 cctggtgacc cgcacggccg atgcgttgca gaccgctcgc cacgctcggc gggctttcga
     1441 cctggacgac gaggtcgacc gcacccatgt cgatgccgag ctcgagggtg ctggtggcga
     1501 cgacagctgg caactgccca gatttcagca tctcttctgt gatgctgcgc tgctcgcgac
     1561 tgaccgagcc gtgatgggcg cgcgcgatct cggtttcgcg gccgcgctcg aagccgatgt
     1621 cgttgaggcg atgggtcagg cgttcggcca gaccgcgcga gttcgcgaac acgatcgtcg
     1681 atcgatgtgc gtcgatcaga tcgctgatgc gctcttcgac gtgtggccag atcgacttgc
     1741 gcggctcttc gtcggtcgcc tcgtcggggg agtccatgtc ttcgacgggc accaccacgg
     1801 atatgtcgaa ggtcttgacg gctggcggtt gcacgactgt cgctggccgc ggaccgctga
     1861 ggaaccgcgc gatctcatcg acgggtcgca ccgtcgccga caggccgatg cgttgcgctg
     1921 gcttctcgag catcgcgtcg agccgttcaa gcgacagtgc gagatgtgca ccgcgtttgg
     1981 tcgcagccac cgcgtgcact tcgtcgacga tgaccgtgtc gattgcctgc aaagactcac
     2041 gtgccgcaga agtgaggatc aggaacagcg actcaggcgt cgttatcagg atgtctggtg
     2101 gcttggtggt caggcgccga cgctcgaccg ccggggtgtc gccggagcgc agcgcgactt
     2161 cgacctttgg ttcgggcaaa ccgaggcggg tggccgcgtg tcgcatgccg acgagaggtg
     2221 ctcgcaagtt gcgctcgacg tcgaccgcga gtgccttcag cggagagatg tagagcaccc
     2281 ggcagcgctt cgacgggtcg gccgggacgg gcgcggcagt gatgcgatcg agtgaccaca
     2341 agaacgcggc gagggtcttg cccgaccccg tcggcgcgac gacgagggtg ttctcgccgc
     2401 ggccgatcgc ctcccacgcc aagacctggg cgggcgtcgg agtcgcgaac gccgagttga
     2461 accaggcttt cgttaccgcc gagaagcct
//
LOCUS       DASWVF010000617         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_501390, whole genome shotgun sequence.
ACCESSION   DASWVF010000617 DASWVF010000000
VERSION     DASWVF010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501390"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGD55_10830"
     CDS             complement(<1..417)
                     /locus_tag="VGD55_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0870882.1"
                     /translation="MTSMPADGVVVRQPAEHVVEVVLDRADALNALSTALAEQLTGVL
                     RDLARDDRVRAVVLSSSNDRAFCVGADLKERAGFDNEQLLAQRPVMRALFSSLLALPM
                     PAIAAVTGFALGGGFELALSCDLIVAEASAVFGLTEV"
     gene            complement(453..1007)
                     /locus_tag="VGD55_10835"
     CDS             complement(453..1007)
                     /locus_tag="VGD55_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0870883.1"
                     /translation="MGMNLSEGEEIVLDLHPNWARLVVPVLVLLVVCLLAGFGVALIP
                     KGGGQLIERWILIGIGVVFVIWLTILPYLRWLTTKYVLTNDRLVIRSGILARHGRDIP
                     LNRINDVSFTETVFERMLRSGTLVVESAGERGQISLTDVPHVEHVQREVYRMVDQHSC
                     RVGPGGGDDGQGNPERPAPDAPAK"
     gene            complement(1044..1913)
                     /locus_tag="VGD55_10840"
     CDS             complement(1044..1913)
                     /locus_tag="VGD55_10840"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0870884.1"
                     /translation="MASSRWTDIERPPLDVRSLTRGLVDYGIWREIRVVDETGSTNAD
                     VAGLARSGAAAGLVLIAEHQSAARGRLDRTWVAPARSGLTFSVLLRPADVTPDVMPDL
                     RPGLPPLPSTAVPPSRWSLLPLVVGFGVASAVRRIAGVEVDLKWPNDAMVGDRKVAGI
                     LAERLDDAVVVGVGINVTLREAELPVPTAISLALAGATCTDRDTLLRAVVRGIGDEYL
                     SWRRVEGDGERSVLPRYRELCTTIGRDVSVDVGSDATVEGRAVGIEADGSLVVETPIG
                     RRSLSAGDVVHVR"
     gene            complement(1917..>4207)
                     /locus_tag="VGD55_10845"
     CDS             complement(1917..>4207)
                     /locus_tag="VGD55_10845"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0870885.1"
                     /translation="RAGDSLATSVAEVRRLHSEDRVASLFAGLLLHPVLTAHPTEARR
                     RAVVTAISRAGAQLDRIDDPRAGAGEQTDARRRLLEEIDILWRTAHLRATSVDPLDEV
                     RTAMAVFDETLFLVVPTLYRGLDHVLLGDRSGTEPPAAPAFFRLGSWVGGDRDGNPTV
                     TAQITREALQIQAGHVLLALERAATRIGRTLTAEAATTPSSRELTQAIEEARHKHPDL
                     LGGLALRAPDEPHRLFVLYTAARIRARRLGEAGGYRRPDELIAQLVLVQESLSKAGAH
                     RLAFGELQHLIWQARTFGFHLAELEVRQHSGVHATALAELASGEVSAMTEEVLETIRT
                     IAWLQGEYGVDACRRYIISFTRSAADIAAVFELAAIATHGNPPVLDVIPLFETQDDLD
                     RSVEILDRALELDAVQERLASNGRRLEVMLGYSDSAKDVGPVSATLALYDAQGALAAF
                     AKRHGITLTLFHGRGGALGRGGGPANRAVLSQAPGSVDGRFKVTEQGEVIFARYGHPV
                     IAHRHLDQVAAAVVLASTPAIEEAVAAAANKHRATFERVSDAARTAYRDLVGTERFAE
                     FFGAVSPLAEIGSLRMGSRPARRTAGSGLADLRAIPWVFAWSQTRATLPGWYGLGSGL
                     EAVGDIDVLRAAYDEWPLFTTMLDNVEMSLAKADRDIAERHLALGGRPELAATILAEF
                     DRTTSWVLRVLNHETLLEGHRVLGRAVELRNPYVDALSHLQLRALEALRAGIDDDEER
                     ERTQRLLLLTVNGVAAGLQNTG"
BASE COUNT          717 a         1473 c         1391 g          626 t
ORIGIN      
        1 gacctccgtg agcccgaaga ccgcactggc ttctgccacg atgaggtcgc acgacagggc
       61 cagctcgaac ccgccgccga gcgcaaaccc agtgacagcg gcgatcgccg gcatcggcag
      121 cgccaggagt gaactaaaca gcgccctcat cacgggacgc tgcgcgagca gttgttcgtt
      181 gtcgaatcca gcccgctctt tcaggtcggc cccgacgcaa aatgcgcggt cgttcgacga
      241 cgaaagcacg accgcgcgca ccctgtcgtc gcgagcgaga tcccgaagga cgccggtcag
      301 ctgctcggcc aatgcggtcg acaaggcgtt cagcgcgtcg gcgcggtcga gcacgacctc
      361 gaccacatgc tccgccggct gccgcaccac gacaccgtcg gcgggcatcg aggtcatgcc
      421 accgacctta cgcgcccgcc acgccgaggc gactacttcg ctggggcgtc cggcgcgggc
      481 cgttccggat tgccctgccc gtcatcgccg cctccgggac cgacgcgaca gctgtgctga
      541 tccaccatcc ggtagacctc acgctggaca tgctcgacat gtggcacgtc ggtgagcgag
      601 atctgaccgc gctcccccgc cgactcgacg acgagagtcc cgctgcgcaa catccgctcg
      661 aagaccgtct cggtgaacga gacgtcgttg atccggttca acgggatgtc gcggccgtgg
      721 cgcgcgagga tgccgctgcg gatcacgagc cggtcgttgg tgagcacgta cttggtcgtg
      781 agccaccgca ggtacggcag gatcgtcagc cagatcacga acaccacgcc gatcccgatc
      841 aggatccagc gctcgatcag ctggccgccg cctttcggga tcagtgcgac cccgaacccg
      901 gcgagcaagc aaacgacgag gagaacgagg acggggacga cgagcctggc ccagttcggg
      961 tgcaggtcaa ggacgatctc ttcgccttct gacaggttca tgcccatgcc ccgcatcttg
     1021 gcatgccggg gcgcctcgca cgctcacctg acgtgaacaa catctccggc cgacagtgat
     1081 cggcgtccga tcggcgtctc tacgacgagc gaaccgtcag cctcgatgcc caccgcgcgg
     1141 ccctcaacgg tcgcatcgct accgacgtcg acgctgacgt cgcggccgat ggtcgtacac
     1201 aactcgcgat acctcggcag cacgctgcgc tcgccgtcac cctctacccg ccgccaactg
     1261 aggtactcgt cgccaatgcc gcgcaccacc gcgcgcagca aggtgtcccg atcggtgcag
     1321 gtcgcacctg ccagtgccag cgaaatcgct gtgggcacag gcaattccgc ttcgcgcaga
     1381 gtcacgttga tgccgacccc cacgaccacc gcatcatcga gccgttcggc gaggatcccg
     1441 gcgaccttgc ggtcgccgac catcgcatcg tttggccact tcaggtcgac ctctaccccc
     1501 gcgatgcgac gcacggccga tgcgacgccg aagccgacca ccagcggcag caggctccac
     1561 cgagagggcg gcaccgccgt cgacgggaga ggtggcaggc ccgggcgcag atcgggcatc
     1621 acgtcgggtg tgacatcggc cgggcgcaac agcacagaga aggtcaagcc cgagcgagct
     1681 ggcgccaccc aggtgcgatc gagccgacca cgcgcggcgc tctggtgctc tgcgatcagc
     1741 acaagcccgg ccgcagcacc cgatcgcgcc agcccggcga cgtccgcgtt cgtcgaacct
     1801 gtctcatcga ccaccctgat ttcgcgccag attccgtagt cgacgagtcc acgcgtaagc
     1861 gagcggacgt cgaggggcgg gcgttcgatg tcagtccagc gtgacgacgc caacgctcag
     1921 ccggtgttct gtaagccggc cgcaactccg ttgactgtca gcagcagcag ccgctgggta
     1981 cgctcgcgtt cttcgtcgtc gtctattccg gcccgcagcg cctcgagcgc gcgcagttgc
     2041 aggtgcgaga gcgcgtcgac gtaggggttg cggagttcga ccgcgcggcc gagcacccgg
     2101 tgcccttcga gcagcgtctc gtggttaagc actcgaagga cccacgacgt cgtgcgatcg
     2161 aactcagcaa gaatcgtcgc ggccaactcg gggcgcccgc caagggccag gtgcctctcg
     2221 gcgatgtcgc ggtcggcttt cgccaacgac atctcgacgt tgtcgagcat cgtcgtgaac
     2281 agcggccatt cgtcgtaggc ggcacgcaag acgtcgatgt cgccgaccgc ctcgagaccg
     2341 ctgcccaggc cgtaccagcc gggcagcgtc gcccgggtct gcgaccacgc gaagacccac
     2401 ggaatcgctc gcaggtcggc cagcccgctg ccagccgtgc gccgggccgg gcgcgagccc
     2461 atgcgcaacg agccgatttc ggccaacggg ctgaccgccc cgaagaactc ggcgaaccgc
     2521 tcggtgccga ccaggtcgcg gtaggcagtg cgcgctgcgt cactgacccg ctcgaacgtg
     2581 gcgcggtgtt tgttcgcggc cgcggccacc gcctcttcga ttgccggggt cgaggccaac
     2641 accaccgccg cggcgacctg atcgaggtgg cgatgggcga tcaccgggtg gccgtagcgg
     2701 gcgaagatga cctcgccctg ctcggtgact ttgaaacggc cgtcgaccga ccctggagcc
     2761 tggctcaaca ccgcgcgatt cgccggacca ccaccgcgcc cgagtgcgcc gccgcgcccg
     2821 tggaacaagg tgagcgtgat gccgtgccgc ttggcaaagg cggccaacgc gccctgcgcg
     2881 tcgtacaggg ccagagtcgc cgagaccggg ccaacgtcct tggccgagtc ggagtagccg
     2941 agcatgacct cgagccgccg gccgttcgag gcgaggcgtt cctgcaccgc atcgagttcg
     3001 agcgcgcggt cgaggatctc gaccgaccgg tcgaggtcgt cttgggtttc gaacaacggg
     3061 atcacgtcga gcaccggtgg gttcccgtgc gtcgcgatcg cggcgagctc gaaaacggcc
     3121 gcgatgtcgg ccgccgatcg ggtgaagctg atgatgtacc gacggcaggc gtcgacgccg
     3181 tattcgcctt gcagccaggc gatcgtgcga atcgtctcga gtacctcttc ggtcatcgcc
     3241 gacacttcac cgctcgcaag ctcggcaagc gccgtcgcgt gcacgccgct gtgctggcgc
     3301 acttcgagtt cggcgagatg aaatccgaac gtgcgcgcct gccagatcag atgctgcaac
     3361 tcaccaaatg ccaaccggtg cgcgccggcc ttcgacagcg actcctggac gagcacgagc
     3421 tgtgcaatca attcatcggg acgccgatag ccgcccgcct cgccaagccg ccgggccctg
     3481 atccgggctg ccgtgtagag gacgaacagt cggtgcggtt cgtcgggcgc ccgcagggcc
     3541 agcccgccaa gcaaatcggg atgcttgtgc cgagcttctt cgatggcctg cgtgagttcg
     3601 cggctcgaag gcgtcgtggc tgcctcggcg gtcagcgtgc ggccgatacg cgtggcggcc
     3661 cgctcgagtg cgagcaacac gtggccggcc tggatctgca atgcctcgcg ggtgatctgg
     3721 gcggtcacgg tcgggttgcc gtcgcggtcg ccgccgaccc aactgccaag acggaagaac
     3781 gctggcgcag caggtggctc ggtgcccgaa cggtcgccga gcagcacgtg atcgagcccg
     3841 cggtacaacg taggcacgac gaggaagagg gtctcgtcga agacggccat ggccgttcgc
     3901 acctcgtcga gcggatcgac cgatgtcgcg cgcagatgtg cggtgcgcca cagaatgtcg
     3961 atctcttcca gcaacctccg ccgcgcatcg gtctgctctc ccgcgcctgc gcggggatcg
     4021 tcgatccggt cgagctgagc accggcacgt gagatcgcag tgacgacggc ccggcgacgt
     4081 gcttcggtcg ggtgggcggt caggaccggg tgcagcagca ggccggcgaa caggctcgcg
     4141 acgcggtctt cgctgtgcag ccgccggacc tcggcgaccg acgttgcaag cgaatcgcca
     4201 gcccgcc
//
LOCUS       DASWVF010000618         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_399478, whole genome shotgun sequence.
ACCESSION   DASWVF010000618 DASWVF010000000
VERSION     DASWVF010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399478"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGD55_10850"
     CDS             <1..717
                     /locus_tag="VGD55_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0870886.1"
                     /translation="RVFDFSRDGIRRSLDESLARFGLDRVDILFLHDPDDHWQQAIDE
                     ALPTLAALRDEGLVRSIGAGMNQSTMLTRFVRESDVDVVLLAGRYTLLDQRALDDLLP
                     ACLERGTSVVLGGVFNSGVLANPDRNVTYDYGQAPRDVIERAARIADICAGHGVDVRA
                     AALQFPVAHRAITTVVIGARSRDEVEQNTQYAGAVIPSQLWDDLRNEGLLRADAPVPT
                     HRPADDSDRPDTDRNVSAPS"
     gene            714..1580
                     /locus_tag="VGD55_10855"
     CDS             714..1580
                     /locus_tag="VGD55_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0870887.1"
                     /translation="MSFFLARLGAAVHERPFVVADGIASSIEMLTTDIDGSFLEGGGI
                     ERVRAAIAEGRLPVAGPLETLGRRFGPPIARPGKVVCVGLNYRDHATETNAAIPTEPI
                     LFMKDPLTVIGPDDDVLVPRRSVKTDWEVELAVIIGRTARYLDDEAAARTCIAGYAIA
                     NDVSEREFQLERGGQWDKGKSCETFNPLGPWFVPADEIADPQALGMRLWVNGTLRQDG
                     TTANMIFGVAHVVHYISQFMVLRPGDVINTGTPAGVAQGQPEPKPFLRAGDVLEVEID
                     GLGSQRQYIGQG"
     gene            1590..2357
                     /locus_tag="VGD55_10860"
     CDS             1590..2357
                     /locus_tag="VGD55_10860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0870888.1"
                     /translation="MTTEFEGLVAVVTGGSSGIGLQTAKLLAARGAKVACLDLVIDGL
                     DPALFGVRADVTDDESVAAAIGIVVSHFGGIDIVINNAGIGARGDVAANDDAEWHRVY
                     DVNVVGLVRVSRAALPYLRASSHAAIVNTGSICAWTGLPARALYSATKGAVLALTLAM
                     AADHVREGIRVNCVNPGTADTPWVGRLLDAAPDPVAERVALEARQPLGRLVTADEVAH
                     AIAYLASPAAASTTGTALAVDGGVFGLRVPAGPPAAP"
     gene            complement(2364..>2781)
                     /locus_tag="VGD55_10865"
     CDS             complement(2364..>2781)
                     /locus_tag="VGD55_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0870889.1"
                     /translation="YEIPGTNEAMTGLDISGFKAMISNVVRDYPNIAVAATTLREVHS
                     ASVNDWGAIAWSADTDFVESTFRRGLEILDRVGGGDSFASGLAFGLMETGSLRSAIEY
                     GAAHGALAMSTAGDTSTANRAEVEALMRGVGAGVQR"
BASE COUNT          473 a          920 c          911 g          477 t
ORIGIN      
        1 cgcgtgttcg acttcagccg cgacggcatc cgcaggtcgc tcgacgagag cctggcccgt
       61 ttcggcctcg accgcgtcga catcctgttc ctccacgacc ccgacgacca ctggcagcag
      121 gccatcgacg aagcgctgcc gacgcttgcg gccctgcgcg acgaaggact agtgcgctcg
      181 atcggtgccg gtatgaacca aagcacgatg ctgacgcggt tcgttcgaga atccgatgtg
      241 gacgtcgtgt tgctcgcggg ccggtacacg ctgctcgacc agcgcgctct tgacgacctg
      301 ttgccagcat gcctcgaacg tggcacctcg gtcgtacttg gcggggtgtt caacagtggc
      361 gtcctggcca accccgaccg caatgtgacc tatgactacg ggcaagcacc ccgcgacgtc
      421 atcgagcgtg ctgcgcggat cgctgatatc tgcgctgggc acggtgtcga cgtccgagct
      481 gctgcgctgc agttcccggt cgcccatcgg gcgatcacga cggttgtgat cggtgcccgc
      541 agccgcgacg aggtcgagca gaacacgcag tacgccggtg cggtgatccc ttcacagctc
      601 tgggacgatc tgcgcaacga gggcctccta cgcgccgatg cgccagtccc tacccatcgc
      661 ccagccgacg acagcgacag acccgacacc gacagaaatg tgagcgcgcc ttcatgagct
      721 tcttcctggc ccggctcggt gcagccgtcc acgagcggcc tttcgtcgtc gccgacggca
      781 tcgcctcgag catcgagatg ctcacgaccg acatcgacgg cagtttcctc gaaggcggcg
      841 gcatcgagcg ggttcgcgca gcaatagccg agggccggct gcccgtagcg ggaccactcg
      901 agactttggg tcggcggttc gggcccccaa tcgcgcggcc gggcaaggtc gtgtgcgtcg
      961 ggctgaacta ccgcgaccac gcgaccgaaa cgaacgccgc aatccccacc gagccgatcc
     1021 tgttcatgaa ggatccgttg acggtgatcg gtccggacga cgatgtgttg gtcccgcgcc
     1081 gttctgtgaa gaccgactgg gaagtcgagc ttgcggtgat catcgggcgc actgccagat
     1141 atctcgacga cgaggccgcg gcacgcacgt gtatcgctgg ctatgcgatc gcgaacgacg
     1201 tgtcagaaag ggagttccag ctcgagcgag gtggccagtg ggacaagggc aagagttgcg
     1261 agaccttcaa tccactcgga ccgtggttcg tccccgctga cgagatcgcc gacccccagg
     1321 cgctcggcat gcgactgtgg gtcaacggca cgttgcgcca agacggcacc accgccaaca
     1381 tgattttcgg cgtcgcgcac gtcgtgcact acatcagtca gttcatggtg ctgcggcctg
     1441 gcgacgtcat caacacgggc acaccggccg gggttgcgca gggtcaaccc gagccgaagc
     1501 cgttccttcg cgccggcgat gttctcgagg tcgaaatcga cggtctcggc agccagcggc
     1561 aatacatcgg acagggttag gagggcacaa tgacgactga gttcgagggt ctcgtcgcgg
     1621 tggtgacggg tggttcgtct ggaatcggtc ttcaaaccgc gaagctgctc gctgcgcggg
     1681 gagcaaaggt tgcctgcctc gacctggtga tcgacggcct cgaccctgcg ttgttcggcg
     1741 tgcgggccga tgtcaccgac gacgaatccg tcgccgccgc catcggcatc gtcgtatcgc
     1801 atttcggtgg catcgacatc gtcatcaaca acgctggcat cggggcgcgt ggcgacgtcg
     1861 ccgctaacga cgacgccgag tggcaccgcg tctacgacgt caacgttgtg gggctggtac
     1921 gagtgagccg cgcggcgctg ccctacctgc gggcctcatc gcacgcggcc atcgtcaata
     1981 ccggatcgat ctgcgcgtgg accggcctgc ccgcgcgggc gctctattcg gccaccaaag
     2041 gcgcagtgct cgcgctgacg ctcgcgatgg cggcggatca cgtccgcgaa ggtatccgcg
     2101 tcaactgcgt gaaccccggg acagccgata cgccgtgggt cggacgactg ctcgatgcgg
     2161 cgcccgatcc ggtcgcggag cgcgtagcgc tcgaggcccg tcagccactc ggtcggctcg
     2221 tgaccgccga cgaggtcgca cacgcgatcg cctacctcgc gtcaccggcg gcagcgtcga
     2281 ctactggcac ggcgcttgct gtcgacggcg gggtgttcgg cctacgggtg ccagctggcc
     2341 cgcctgcggc tccatagctg cgcctagcgc tgcacgccgg cgccgacacc cctcataagc
     2401 gcttcgactt ccgcgcgatt ggcggtcgac gtgtcaccgg cggtcgacat ggcgagcgca
     2461 ccgtgcgcag caccgtattc gatggcactc ctgagcgaac cggtctccat caaaccgaag
     2521 gccagaccgg aggcgaagga gtcaccgccg ccgacgcgat cgaggatttc cagtccgcga
     2581 cggaaggtgg actcgacaaa gtcggtgtca gccgaccatg cgatggcacc ccagtcgttg
     2641 acgctcgcgc tgtgtacctc gcgtagtgtc gtggccgcga cagcgatatt cgggtagtcg
     2701 cgcacgacgt tggagatcat cgccttgaac ccgctgatat cgaggcctgt catagcctcg
     2761 ttcgttccgg ggatttcgta g
//
LOCUS       DASWVF010000619         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_450619, whole genome shotgun sequence.
ACCESSION   DASWVF010000619 DASWVF010000000
VERSION     DASWVF010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450619"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VGD55_10870"
     CDS             complement(<1..1596)
                     /locus_tag="VGD55_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0870890.1"
                     /translation="MVARIIAVLLLARGVYGDPARLLARRASGGFPISGQPLRLGEFV
                     ATFALAQDNAFGQPLESQLRSCLLAAAICEEAGFDAQLSETVYWVALLRYIGCTGHAH
                     EVATLFGDEIAIRAQTLVHDAANPAEVMRDVFAYATAGHSPEEQEQIVAAIKVGAHDW
                     AVHNFASGCEVGDMLARRLDMNDDVLDALRFTFERWNGNGFPAGAKGEEIPLAMRVVH
                     LSHDMEAIARLFSPAQALETVRERRDGTYDPELADIFSTHGAGFLERLGKTDPWDEVL
                     ALEPRPRRILEGADLDNALEVAADFIDLKSPYMAGHSRRCALLSADAAALLGCSADEI
                     ATVRRAALVHEFGTTAIPNSILDKTGGLTRAEFDRVEIHPMLTEQMLRRTPALAALNP
                     VAAAHHEKVDGSGYHKRVRADAVSTCGHILATADMYVGLTTDRADRPAFGPHLAAAEL
                     RRLVVTGVLDQRATSAVLQAAGHADDGSRTTPAPKHVSGLTPREVEVLRLAARGLTTG
                     EIASQLFISPKTADHHIQHVYGKI"
     gene            1580..1852
                     /locus_tag="VGD55_10875"
     CDS             1580..1852
                     /locus_tag="VGD55_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870891.1"
                     /translation="MILATTKVENYERFLEIFSTKGAEKRALHGSKGATVFRDPVEDD
                     RVWVLFDWDADGWKKFVSDPEVPAILQEAGHIGKPQAAELGGRYAA"
     gene            1896..>2714
                     /locus_tag="VGD55_10880"
     CDS             1896..>2714
                     /locus_tag="VGD55_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEY0870892.1"
                     /translation="MNETVPTRRPIKVGVITDQTGPLSVMGIANANVAAMVIDDINAE
                     GGLLGRPLELHLEDSETTDSAGEAKARKLIEQDRVDVIFGGIYSSMRQAIKGPAVVEG
                     KTLYVYPEQYEGQESDPLIFCTGPVPAQQIDPFIPWLMRQTGARKFYLPSADYIWPHV
                     LNARVREVVVANGGQIVGEEYFPLDHMDYEATIDAIVSTGAEVVFNTLVPPGLVPFLQ
                     GLHDSGFVKNGGKLVCTYFDENVLDMLPPEQVEGIYSCLDYYQDVSDPFSRELLD"
BASE COUNT          482 a          858 c          900 g          474 t
ORIGIN      
        1 gatcttcccg tacacgtgct ggatgtggtg gtcggcggtt ttgggtgaga tgaagagctg
       61 gcttgcgatc tcgccggtcg tcagcccgcg ggcagccaag cggagcacct cgacctctcg
      121 cggtgtgagc ccactcacgt gcttgggcgc aggagtggtc cgggagccgt cgtcggcgtg
      181 gccggcggct tgcaagaccg cactggtcgc gcgctgatcg agcacgccag ttacgacgag
      241 gcgccgaagt tcggcggccg ctaagtgagg cccgaaggcc ggccgatctg cgcggtcggt
      301 ggtgagaccc acgtacatat cggcggtcgc caggatgtgc ccgcaggtgc tgaccgcgtc
      361 ggctcgtact cgcttgtggt agccggagcc gtcgaccttc tcgtggtgcg cagcggcgac
      421 tggattcagt gccgccagtg ccggcgtccg gcgcagcatc tgctcggtca gcatggggtg
      481 gatctcgacc cggtcgaact cagcgcgggt gaggccgccc gtcttgtcga ggatcgagtt
      541 cggtatcgcc gtcgtgccga actcgtggac gagtgccgct cggcgcaccg tcgcgatctc
      601 gtcggcggag caaccgagca gcgccgcagc gtcggcactg agtagcgcgc atcgacggct
      661 gtggcccgcc atgtacggcg acttaaggtc gatgaagtcg gccgcgacct caagggcgtt
      721 gtcgaggtcg gcgccctcga gaatccggcg cggtcttggt tcgagcgcca gcacttcgtc
      781 ccacggatct gtcttcccga gtcgctcgag aaatccagct ccgtgcgtgg aaaagatatc
      841 ggccagctcg gggtcgtaag tgccgtcgcg tcgctcgcga acggtctcaa gggcctgcgc
      901 gggggagaag agacgagcga tcgcttccat gtcgtggctg agatgcacga cccgcatggc
      961 gagtggaatc tcttcgcctt tcgcgccggc cgggaagccg ttgccgttcc atcgttcgaa
     1021 cgtgaagcgc agtgcgtcga gcacgtcgtc gttcatgtcg agtcgtcgcg ccagcatgtc
     1081 gccgacttcg catcccgacg cgaagttgtg gacggcccag tcatgcgcgc cgactttgat
     1141 cgccgcgacg atctgctctt gctcctccgg cgagtgtccg gcagtcgcgt aggcgaacac
     1201 gtcgcgcatg acctcggccg ggttcgcggc gtcgtgcaca agcgtctgcg cgcgaatcgc
     1261 gatctcgtcg ccgaacaagg tggcgacctc gtgcgcatgc cctgtgcacc ctatgtagcg
     1321 cagcagcgcg acccagtaga ccgtttcgga cagttgcgcg tcgaagcctg cctcctcgca
     1381 gatcgccgcc gccagcaggc acgaacgcag ctgcgactcg agcggttggc cgaacgcgtt
     1441 gtcctgcgcg agggcgaatg tcgcgacgaa ctcgccgagc cgcagcggtt gaccagaaat
     1501 agggaaacct cccgatgccc ggcgtgcgag gagacgcgca ggatcaccgt acacaccccg
     1561 agcaagaagg agaacggcga tgatcctggc aaccacgaaa gtcgagaact acgagcgctt
     1621 cctcgagatc ttctcgacga agggcgccga gaagcgcgcg ctgcatggct ccaagggcgc
     1681 gaccgtgttc cgcgacccgg tcgaggacga ccgggtttgg gtcctcttcg attgggacgc
     1741 cgacggctgg aagaagttcg tgtccgaccc cgaagtcccg gcaatcctcc aggaagccgg
     1801 ccacatcgga aagccgcagg ccgccgaact cggtggtcgt tacgccgcct gacgacagcc
     1861 ctccaagccc gagctggcat cactaaggag tggatatgaa cgaaacagta cccacccggc
     1921 gtccgatcaa agtcggtgtc atcacggacc agacgggtcc gctctcagtg atgggcatcg
     1981 cgaatgcgaa cgtcgccgcg atggtgatcg acgacatcaa cgctgagggc ggtctcttgg
     2041 gacgcccgct ggagttgcat ctcgaggaca gtgaaacgac cgacagcgcc ggggaggcga
     2101 aggcccgcaa gctcatcgag caggatcggg tcgatgtcat cttcggcggc atctacagct
     2161 ctatgcgcca ggccatcaaa ggtccggccg tcgtagaggg caagacgctc tatgtgtatc
     2221 cggagcagta cgagggacag gaaagcgatc ccctgatctt ctgcaccggt ccggtaccgg
     2281 ctcagcagat cgacccgttc attccgtggc tgatgcgaca gaccggcgcg cgaaagtttt
     2341 acctgccgtc ggccgactac atctggccgc acgtgctgaa cgccagggtc cgtgaggttg
     2401 tcgttgcgaa cggtggccag atcgtgggcg aggagtactt cccgctcgac cacatggatt
     2461 acgaggcaac catcgacgcc atcgtctcga ccggcgccga ggtcgtgttc aacactctcg
     2521 ttccgcccgg cctcgtgccg ttcctccagg gtctgcacga ctctggcttc gtcaagaacg
     2581 gcggcaagct ggtctgcacg tacttcgacg agaacgtctt ggacatgttg ccgcccgagc
     2641 aggtcgaggg catctacagc tgcctcgact actaccaaga cgtcagcgat cccttcagcc
     2701 gggaactgct cgac
//
LOCUS       DASWVF010000620         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_195714, whole genome shotgun sequence.
ACCESSION   DASWVF010000620 DASWVF010000000
VERSION     DASWVF010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195714"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VGD55_10885"
     CDS             <1..1777
                     /locus_tag="VGD55_10885"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0870893.1"
                     /translation="KPARYDRAVILRMSTLFLRTLREDPAEAEVPSHRLLVRAGYVRR
                     IAPGIYSWLPLGYEVFRNVERIVREEMDRMGAQEVHFPALIPREIYEASGRWSDYGET
                     LFRLKDRKGADYLLAPTHEELFTLLVKGEYSSYKDYPVTLYQIQTKYRDEARPRAGIM
                     RGREFVMKDSYSFDLDDEGLANSYAAHRVTYQKTFERLGLDYRIVFAVSGAMGGSASE
                     EFLAPTESGEDTFVQCANCDYAANTEAVEIAVPAPIERADHPPSQVLDTPGTPTIQTL
                     VDHLNALGLGREFTAADTLKNLVLKTQAPGSTKWELLVIGVPGDREVDLKRVQGQLEP
                     VQVAQAEPADLAAEPRLVKGYIGPQILKEIGIRYLVDPLVVPGSAWVTGANVENQHAA
                     AVVRGRDFEPDGEIGAVEIRDGDLCARCGGRLSIARGIEIGHIFQLGRKYADVFGLDA
                     LGPDAKPIRITMGSYGLGVSRAVAALAEQHHDELGLVWPASVAPADVHLVAAGKEDQL
                     TAAEKLAHDFAEAGVRVLFDDRGGVSVGVKFKDAELIGVPLIVVVGRGLVDGLVELRD
                     RASGDRGDVALDSVVAHVKRKLTVS"
     gene            1863..2471
                     /locus_tag="VGD55_10890"
     CDS             1863..2471
                     /locus_tag="VGD55_10890"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY0870894.1"
                     /translation="MHVTLYGFVLFLHILTAIVAFMMAAIVHAGLPVLARARDVREMR
                     SWAAILHRLEPLFPVAALLLLGFGAWLVHLSDGEIKFSDSWLLTGLITLVVVEAVAGA
                     KLAPKAKLAVRLVEEAADGPVPDAVRQAALEPWIWYPTHIATFGFGAVVYLMAAKPNG
                     AWPPVIVIIGVLIGVAAARLQLRALTAPPAAATSSLQTEAAK"
     gene            2624..>4078
                     /locus_tag="VGD55_10895"
     CDS             2624..>4078
                     /locus_tag="VGD55_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0870895.1"
                     /translation="MRRQHLFATVALAATISLALAACSSSKPKATGGSSGSTAATGKL
                     QQVVLGTTDKVISIDPAGAYDEGSWVLQWNIYQALLKIKAGTSDVVPDAASACTWTDT
                     VTYTCKLNPGLVFSNGDPLDAAAVVFSINRVIKINDPNGPSSLLSSMKSVAAQGTDTV
                     VFTLNAPNSVFPYILTTGAGNIVDPKVFPADKLLPDGPGDIGSGVYTLSKYTANQQAV
                     LTPNPKYKGDDVLANKGFIVHYEQTPATLKLDVEQGNVDVAWRSLSPTDIASLQTETS
                     KVKVVKGNGIEIRYLVFNTSLPPAKDLATRQAVAYSIDRNAIANTVYNGTVTPLYSMV
                     PAGLTGANQAYKDLYGATPDAAKAKAVLAAAGVTTPVDITIWWTPSHYGAVSGDEYTE
                     IKRELEATGLFKVTLQSAEWDAYSKAYPKDQYAAFELGWFPDYPDADDYLAPFYLKGG
                     FYNNHYDNPAIDTLLTQEEGETDATKRLGELQQIQ"
BASE COUNT          730 a         1373 c         1300 g          675 t
ORIGIN      
        1 aaagcccgcc agatacgatc gggccgtgat cttgcggatg tcgaccctgt tccttcgcac
       61 gttgcgtgag gaccccgccg aagccgaggt ccccagccac cgcctcctcg tgcgcgccgg
      121 ctacgtcaga cgcatcgctc cgggcatcta ctcctggctg ccccttggct acgaggtgtt
      181 ccgcaacgtc gagcgcatcg tgcgcgagga gatggaccgt atgggggcgc aggaggtcca
      241 ttttcctgcg ctgatcccgc gagagattta cgaggcgagc ggccgctgga gtgattacgg
      301 cgagaccctc tttcgcctca aggaccgcaa gggcgccgac tacctgctcg ccccgaccca
      361 cgaggagctc ttcacgctgc tcgtgaaggg cgagtactcc tcatacaagg actacccggt
      421 cacgctctac cagatccaga cgaagtaccg cgacgaagct cgcccgcggg ccggcatcat
      481 gcgcggtcgc gagttcgtga tgaaagacag ttacagcttt gacctggacg acgaggggct
      541 ggcgaactcc tacgccgccc accgcgtgac ctaccagaag accttcgagc ggctcggcct
      601 cgactaccgg atcgtcttcg cggtgtccgg agcgatgggc ggatccgcgt ccgaggagtt
      661 cctcgcaccg accgagtcgg gcgaagacac ctttgtgcag tgcgcgaact gcgactacgc
      721 ggcgaacacc gaggctgtcg agatcgccgt gcctgccccg atcgagcgcg ccgatcaccc
      781 gccatcgcaa gtgctcgaca cccctggcac cccgacgatc cagacgctgg tcgaccacct
      841 gaacgctctg ggcctcggtc gcgagttcac ggccgccgac acgttgaaga acctcgtgct
      901 gaagacccag gctcccggca gtaccaaatg ggagctgctc gtcatcggcg ttccgggcga
      961 ccgcgaggtc gacctcaagc gggtgcaggg ccaactcgaa ccggtccagg tcgcccaggc
     1021 cgagcccgcc gacctggcgg ccgaaccgcg cttggtgaag ggctacatcg gcccgcaaat
     1081 cctgaaagag atcggcatcc gttacctcgt cgatccgctc gtggtgccgg gcagcgcatg
     1141 ggtcaccggg gccaatgtcg agaaccagca cgccgcggcc gtggtgcgcg gtcgcgactt
     1201 cgaacctgac ggtgagatcg gtgccgtcga gattcgcgac ggcgatctgt gcgcgcgctg
     1261 cggcgggcgg ttgtcgatcg cgcgcggtat cgagatcggc cacatcttcc agctgggccg
     1321 caagtacgcc gacgtcttcg ggctcgacgc gctcgggccg gatgccaagc cgatccgcat
     1381 caccatgggg tcatacggtc tcggcgtctc gcgggcggtt gccgcacttg ccgagcagca
     1441 ccacgacgag ctcggcctgg tctggccagc ttcggtcgcg ccggccgacg tccaccttgt
     1501 ggccgcaggc aaagaggatc agctcacggc cgccgagaag ctggcccacg acttcgccga
     1561 agccggagtg cgggtgttgt tcgacgatcg gggtggtgtg tcggtcggtg tgaagttcaa
     1621 ggacgccgag ctgatcggcg tcccgctgat tgtcgttgtc gggcgaggcc ttgtcgacgg
     1681 gctcgtcgag ctgcgtgacc gggcgagtgg tgatcgtggc gacgtcgcgc tcgactccgt
     1741 cgtcgcacac gtcaagcgca agctgaccgt ttcctgaatc cgaccacgcc accctctacc
     1801 aggggtgccc gggccgcact atcgttccga ccgtctgcgg tacccatggt atggaggtct
     1861 acatgcatgt cacgctctac gggttcgtgc ttttcctgca catcttgacc gcgatcgttg
     1921 cgttcatgat ggcggcgatc gtgcacgccg gactgcccgt tctggcgcga gcgcgtgacg
     1981 tgcgtgagat gcggtcgtgg gccgcgatcc tgcaccggct cgaaccgctg ttcccggtcg
     2041 ccgcgctgct ccttctcggg ttcggtgcgt ggctcgtgca cctgtcggac ggcgaaatca
     2101 agttcagcga cagctggctg ctcacaggtc tgatcaccct cgtcgtggtc gaggcagttg
     2161 ccggcgcgaa gctggcgccg aaggccaagc tggccgtgcg cctcgtcgag gaagccgccg
     2221 acggtccggt gcccgacgcc gtccggcagg ccgctctcga gccctggatc tggtacccga
     2281 cgcacatcgc gacgttcggc tttggtgcgg tcgtgtacct catggccgcg aagcccaacg
     2341 gtgcgtggcc gccggtgatc gtcatcatcg gagtgctgat cggggtcgcg gcggcgagac
     2401 ttcagctgcg agcgctgacg gctccgcccg ccgccgcgac gtcgtcgctc caaaccgagg
     2461 ctgccaagta gttccgcaga taacgatttg gacagccgat cgtaagggac cttcctaaag
     2521 ccgtcgtaaa accgcttagc tgaccagcac acgccgccga ccgcggcgag ttctcgcgcg
     2581 cgatgccgcc accctggtgt cgcgctgaca tggaggcccc tgaatgcgac gacagcacct
     2641 gttcgccacc gtggctctgg cagccaccat cagccttgcg ctcgctgcat gcagctcgag
     2701 caagccgaaa gcgaccggcg gctcgagcgg gagtaccgcg gcgaccggaa agctccagca
     2761 ggtcgtgctc ggtaccaccg acaaggtgat ctcgatcgac ccggccggcg cctacgacga
     2821 gggttcctgg gtgctgcagt ggaacatcta ccaggcattg ctgaagatca aggcgggcac
     2881 gtccgacgtc gtccccgacg ctgcgagcgc ctgcacctgg accgacaccg tcacctacac
     2941 ctgcaagctg aacccgggcc tggtcttcag caacggtgac ccgctcgacg cggctgccgt
     3001 cgtgttctcg atcaaccggg tcataaagat caacgacccg aacggaccgt cgagcctgct
     3061 ctcctcgatg aagagcgtcg cggctcaggg caccgacacc gtcgtgttca cgctgaacgc
     3121 cccgaactcg gtcttcccgt acatcctcac gacgggtgcc ggcaacatcg tcgacccgaa
     3181 ggtgttccca gccgacaagc tgctccccga tggccctggc gacatcggat cgggcgtcta
     3241 caccctgagc aagtacacgg cgaaccagca ggcggttttg acgccgaacc cgaagtacaa
     3301 gggtgacgac gtgctcgcca acaaggggtt catcgtgcac tacgagcaga ccccagcaac
     3361 cctcaagctc gacgtcgagc agggcaacgt cgacgtcgca tggcgcagcc tgagcccgac
     3421 cgacatcgcc tcgctccaga ccgagaccag caaggtcaag gtcgtcaagg gcaacggcat
     3481 cgagatccga tacctggtgt tcaacacctc gttgccgccg gcaaaggatc tcgcgacaag
     3541 gcaggcggtc gcgtactcga tcgaccgcaa cgcgattgcg aacaccgtct acaacggcac
     3601 ggtcacgccg ctgtactcga tggtgccggc gggtctgacc ggggccaacc aggcctacaa
     3661 ggatctctac ggtgcgacgc ccgatgcggc gaaggccaag gcggtccttg cggcagctgg
     3721 tgtgacgacg ccggtcgaca tcacgatctg gtggaccccg tcgcactacg gcgcagtgtc
     3781 gggcgacgaa tacaccgaga tcaagcgcga gctcgaagca accggcttgt tcaaggtgac
     3841 cttgcagagt gcggagtggg acgcgtacag caaggcctac ccgaaggacc agtacgcagc
     3901 cttcgagctg ggctggttcc cggactaccc ggacgctgac gactacctgg cgcccttcta
     3961 cctcaagggc ggtttctaca acaaccacta cgacaacccc gcgatcgaca ctctcctcac
     4021 gcaggaagag ggcgagaccg acgccaccaa gcggttgggt gaactccagc agatccag
//
LOCUS       DASWVF010000621         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_221450, whole genome shotgun sequence.
ACCESSION   DASWVF010000621 DASWVF010000000
VERSION     DASWVF010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221450"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGD55_10900"
     CDS             <1..553
                     /locus_tag="VGD55_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0870896.1"
                     /translation="RGVNVTAEVTPHHLLLTDDLAATYDPLYKVNPPLRSRDDVEAVR
                     RGLADGTIDAVATDHAPHSLEDKETEWQAAASGMLGLETALSVVAQTMVETGLLEWAG
                     VVDRMSIRPARIGRLNGQGQPIAVGAPANVVLIDPWARRVIDPRALASKSRNTPYAGR
                     ELPVRVVHTFLRATPTVLDGKPA"
     gene            550..1677
                     /gene="carA"
                     /locus_tag="VGD55_10905"
     CDS             550..1677
                     /gene="carA"
                     /locus_tag="VGD55_10905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY0870897.1"
                     /translation="MTVLTSRRPPAVLVLEDGRTFHGQSYGAVGGTFGEAVFATGMTG
                     YQETLTDPSYHRQVVVMTAPHIGNTGVNDDDAESTRIWVAGYVVRDPARVPSNWRSRR
                     TLDEELAAQGVVGISGIDTRALTRHLRERGAMRVGISTDSADVDGLLDRVRATDRMEG
                     ADLAGQVSTVNPYVVPAIGERRFTVAAIDLGIKSMTPHRMAQRGMQVHVLPATSSIDD
                     VLAVEPDGVFFSNGPGDPAASVGPVQLLSEVLERRLPFFGICFGNQLLGRALGFGTFK
                     LNYGHRGINLPVMDKLTGKVEVTAHNHGFAVEAPLEGEHPTPFGRARVSHVCLNDEVV
                     EGLECRDTPAFSVQYHPEAAAGPHDAAYLFDRFGDLMKAGR"
     gene            1677..>3825
                     /gene="carB"
                     /locus_tag="VGD55_10910"
     CDS             1677..>3825
                     /gene="carB"
                     /locus_tag="VGD55_10910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0870898.1"
                     /translation="MPKRTDLKSVLVIGSGPIVIGQACEFDYSGTQACRVLRAEGLRV
                     SLVNSNPATIMTDPEFADATYVEPITPEFVEMIIAKERPDALLATLGGQTALNTAVAL
                     HEAGVLEKYNVELIGANIEAIQAGENRQRFKEIVAAVGAESARSVICHTLADCLASTD
                     ELGYPVVVRPSFTMGGAGSGIAYNETDLRRIAGAGLQASPTTEVLLEESILGWKEYEL
                     ELMRDRKDNVVVVCSIENVDPMGVHTGDSITVAPALTLTDREYQHLRDIGIDVLRAVG
                     VDTGGCNIQFAVQPETGRVIVIEMNPRVSRSSALASKATGFPIAKIAAKLALGYTLDE
                     IPNDITRETPASFEPSIDYVVVKVPRFAFEKFPGVDDTLTTTMKSVGEAMAIGRNFTE
                     ALQKALRSLEQTGSSFRWDGDLGEREALVEKSKRPTDGRIVTVQQAIRAGASVDELHD
                     ATGIDKWFLDQILLLDEVASDLAQAESLDIETLRHAKRHGFSDAQIAAIRGVSEVSVR
                     DARHAIGLRPVYKTVDTCAAEFAANTPYHYSSYDDETEVVPRTNPAVLILGSGPNRIG
                     QGVEFDYSCVHASFALREAGFETVMINCNPETVSTDYDTSDRLYFEPLTLEDVLEVVH
                     AERIAGPVVGVIVQLGGQTPLRLAQALKDAGVPIIGTTPEAIHLAEDRGAFGQVLARA
                     NLPAPRHGTATSFDEAKAIADEIGYPVLVRPSYV"
BASE COUNT          664 a         1245 c         1280 g          636 t
ORIGIN      
        1 gcgcggggtg aatgtcaccg ccgaggtcac gccgcatcac ctgctgctca ctgatgacct
       61 ggcggccacc tacgacccgc tctacaaggt caatccgccg ctgcgcagcc gtgatgatgt
      121 cgaagcggtc cgtcgcggac tcgccgacgg cacgatcgac gccgtcgcga ccgaccatgc
      181 tccgcattct ttggaggaca aggaaaccga gtggcaggcg gcagcttcgg ggatgctcgg
      241 tctcgagacg gctctctcgg tcgtcgcgca aacgatggtt gagaccggcc tgctcgaatg
      301 ggccggcgtc gttgaccgca tgtcgatacg ccctgcccgc atcgggcgct tgaacggcca
      361 agggcagccc atcgcggtcg gcgcgccggc caacgttgtt ctcatcgatc cgtgggcgcg
      421 ccgcgtcatc gaccctcggg cgcttgcgtc gaaatcccgc aacacccctt acgccggccg
      481 ggagctgcca gtgcgcgtcg tccacacctt ccttcgcgcg actccgaccg tgctcgacgg
      541 gaaacccgca tgacggtgct cacttctcgg cgtccgcccg cagttctcgt tcttgaggat
      601 ggccgcacgt tccacggcca gtcctacggc gcagtcggtg ggaccttcgg cgaagcggtg
      661 ttcgcgaccg gcatgaccgg gtaccaggag acgctcacgg atccgagcta ccaccggcag
      721 gtggtcgtga tgaccgcgcc gcacatcggc aacaccgggg tgaacgacga cgacgcggaa
      781 tcgacgcgca tctgggttgc ggggtatgta gttcgcgacc cggcgcgggt gccgtcgaac
      841 tggcgctcga ggcgcaccct cgacgaagag cttgcggcgc aaggcgttgt cggcatcagc
      901 ggcatcgaca cgcgcgccct gacgcggcac ctgcgcgagc gcggcgcgat gcgggtaggc
      961 atctcgacag acagcgcgga cgtcgatggg ctgctcgatc gggtccgcgc gacggatcgg
     1021 atggaaggcg ccgacctcgc cggccaggtg tcgacggtaa acccctacgt cgtgccagcg
     1081 atcggcgagc ggcgctttac ggtggctgcg atcgaccttg gcatcaagtc gatgacgccg
     1141 caccgcatgg ctcaacgtgg catgcaggtg cacgtgcttc ccgcgacgtc gagcatcgac
     1201 gacgttctcg cggtcgagcc cgacggagtg ttcttctcca acggcccggg cgacccggcc
     1261 gcgtcggtag ggcctgtgca gttgctgtcg gaggtgctcg aacgcaggct gccgttcttc
     1321 ggcatctgtt tcggtaacca gttgctcggc cgcgcgctgg gcttcggcac cttcaagctc
     1381 aactacgggc accgcggcat caaccttccg gtgatggaca agctcaccgg caaggtcgaa
     1441 gtaaccgcgc acaaccacgg cttcgctgtc gaagcgccgc tcgagggcga gcaccccacg
     1501 ccgttcggcc gggcgcgggt gagccatgtg tgcctcaacg acgaggtcgt cgaagggctc
     1561 gagtgccgtg atacgccggc attctcggtg cagtaccacc ccgaggcggc ggccggcccg
     1621 cacgacgctg cctacctgtt cgatcgcttc ggtgacctga tgaaagcggg gcgctgatgc
     1681 cgaagcgcac cgacttgaag tcggtcctgg tgatcggctc tgggcccatc gtgatcgggc
     1741 aggcctgcga gttcgactac tcaggtaccc aggcgtgccg ggtgttgcga gccgagggct
     1801 tgcgggtttc gctggtgaac tccaacccgg ccacgatcat gaccgatccc gagttcgccg
     1861 acgccacgta cgtcgagccc atcacgcccg agttcgtcga gatgatcatc gccaaggagc
     1921 ggcccgacgc gttgctggcg accctgggtg ggcagaccgc gctgaacacc gccgtagcgc
     1981 tgcacgaggc cggcgtgctc gagaagtaca acgtcgagtt gatcggcgcg aacatcgagg
     2041 cgatccaggc gggggagaac cggcagcggt tcaaggagat cgtggccgcc gtcggtgccg
     2101 agtcggcacg ctcggtgatt tgccacaccc tggccgactg tctcgcgtcg actgacgaac
     2161 tcgggtaccc cgtcgtcgtc cggccctctt tcaccatggg tggtgcgggg tctggaatcg
     2221 cgtacaacga gaccgacctg cgtcgcattg cgggtgccgg actccaggcg agcccgacca
     2281 ccgaggtgct gctcgaggag tcgatcctcg ggtggaagga atacgagctc gagctgatgc
     2341 gcgaccgcaa ggacaacgtc gtcgtggtct gctcgatcga gaacgtcgac cccatgggcg
     2401 tgcacacggg tgactcgatc acggtcgcgc cggcgcttac gctgaccgac cgcgagtacc
     2461 agcacctgcg cgacatcggc atcgacgtgt tgcgggcagt cggcgtcgac accggcggct
     2521 gcaacatcca gttcgccgtg cagcccgaga ccggtcgggt catcgtgatc gagatgaacc
     2581 cgcgggtgtc gcgatcctcg gcattggcga gcaaggcgac cggatttccc atcgccaaga
     2641 ttgccgcaaa actcgcgctc gggtacaccc tcgacgagat cccgaacgac atcacccgcg
     2701 agacaccggc gagcttcgag cccagcatcg actacgtcgt cgtcaaggtt ccgaggttcg
     2761 cgttcgagaa gttcccgggt gtcgacgaca cgttgaccac gacgatgaag agcgtcggag
     2821 aggccatggc gatcggccgc aactttaccg aggcgttgca gaaggctctg aggtcactcg
     2881 agcagacagg gtcgtcgttt cggtgggacg gcgatctggg tgagcgcgag gcgctcgttg
     2941 agaagtcgaa acgacccacc gatggccgga tcgtgacggt gcagcaggcg atccgggccg
     3001 gcgcgagtgt tgacgaactg cacgacgcga caggcatcga caagtggttc ctcgatcaga
     3061 tcctcctgct cgacgaagta gcatccgacc tcgcacaggc agagtctctc gatatcgaga
     3121 cacttcggca cgccaaacgg catggattct ccgatgcgca gatcgcggcg atccgaggcg
     3181 tcagtgaggt cagtgtgcgt gacgcgcggc acgcgatcgg cctgcggccg gtgtacaaga
     3241 ccgtcgacac ctgcgccgcc gagtttgccg cgaacacgcc gtaccactac tcgagctatg
     3301 acgacgagac cgaggttgtg ccgcgaacga atcctgcggt gctgatcctc gggtcgggtc
     3361 ctaatcgcat cgggcagggc gtcgagttcg actattcctg cgtccacgca agcttcgctt
     3421 tgcgcgaggc cgggttcgag accgtgatga tcaactgcaa ccccgaaacg gtgtcgaccg
     3481 actacgacac gagcgaccga ctttacttcg agccactgac ccttgaggac gtgctcgagg
     3541 tcgttcacgc cgagaggatt gctggccccg tggtcggggt catcgtgcaa ctcggcggcc
     3601 agaccccgct ccggcttgcc caggcattga aggacgccgg cgtgccgatc atcggcacga
     3661 cccccgaggc gatccacctg gccgaagacc gcggcgcttt cggtcaggtg ctggcgcggg
     3721 cgaacttgcc ggcgccacgg cacggtacgg cgacatcttt cgacgaggcc aaggcgatcg
     3781 cagacgagat cgggtacccc gtgctggtgc ggcccagtta cgtgc
//
LOCUS       DASWVF010000622         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_373948, whole genome shotgun sequence.
ACCESSION   DASWVF010000622 DASWVF010000000
VERSION     DASWVF010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373948"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /gene="rplF"
                     /locus_tag="VGD55_10915"
     CDS             <1..234
                     /gene="rplF"
                     /locus_tag="VGD55_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0870899.1"
                     /translation="DLEFALGFSHPVNIPAPDGITFEVQAPTRFVVRGIDKQQVGEVA
                     ANIRKLRKPEPYKGKGVRYQGEVVRRKVGKAGK"
     gene            237..626
                     /gene="rplR"
                     /locus_tag="VGD55_10920"
     CDS             237..626
                     /gene="rplR"
                     /locus_tag="VGD55_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0870900.1"
                     /translation="MPVGLISRRKARGSTKAALISRRHFRVRKKVSGTALRPRLVVNR
                     STRHVFAQVVDDTTGRTLASASTMEADVRATKGDKTAKARKVGELLAKRATAAGVGPV
                     VFDRGGFQYHGRVAAIADGAREGGLTL"
     gene            694..1347
                     /gene="rpsE"
                     /locus_tag="VGD55_10925"
     CDS             694..1347
                     /gene="rpsE"
                     /locus_tag="VGD55_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0870901.1"
                     /translation="MPGPQRRGPGGAAGAGAGAAGASAGSGDGRRDNRRDNRRPEGAA
                     EKTQYIERVVAINRVAKVVKGGRRFSFTALVVVGDGDGSVGIGYGKAKEVPAAIAKGV
                     EEAKKSFFKVPRIQHTIPHPITGEAAAGVVLLRPASPGTGVIAGGPVRAVLECAGIHD
                     VLSKSLGSDNAINVVHATVAALKGLMRPEEVAARRGLPLEDVAPAALLRARAAGMGA"
     gene            1349..1531
                     /gene="rpmD"
                     /locus_tag="VGD55_10930"
     CDS             1349..1531
                     /gene="rpmD"
                     /locus_tag="VGD55_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009190145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0870902.1"
                     /translation="MGRLKVTQTRSEIGGTSRQRNTLRTLGLKRMRDTVVKEDRPEIR
                     GMIKTVTHLVAVEEVD"
     gene            1612..2046
                     /gene="rplO"
                     /locus_tag="VGD55_10935"
     CDS             1612..2046
                     /gene="rplO"
                     /locus_tag="VGD55_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0870903.1"
                     /translation="MKVHHLRPAPGSHKPKTRVGRGEASKGKTAGRGTKGTKARYQVP
                     ERFEGGQMPLHMRLPKLKGFTNRFRTEYQVVNLEWIAAHYPKGGAVTPDDLVAKGAVR
                     KGELVKVLGDGEIGVKLQVSAHGFSASAKDKIEAAGGSATKL"
     gene            2186..>2675
                     /locus_tag="VGD55_10940"
     CDS             2186..>2675
                     /locus_tag="VGD55_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0870904.1"
                     /translation="MNYALLRAFGQAFRTPDLRKKMLVVLFIVLLFRLGSAIPAPGVS
                     ETNIHACLVQVTGGNNANVYTLINLFSGGALLKLSILALGIMPYITSSIIIQLLVVVI
                     PRLERLKQEGQSGPAKLTQYTRYLTIGLAILQSSAFVALARTPGQIFTGCSTPIIPND
                     GWQ"
BASE COUNT          496 a          855 c          908 g          416 t
ORIGIN      
        1 gacctcgagt tcgcgctcgg cttctcgcac ccggtcaaca tccccgcgcc cgatgggatc
       61 accttcgagg tgcaggcacc gaccaggttc gtggtgcgcg ggatcgacaa gcagcaggtc
      121 ggcgaggttg cggcgaacat ccgcaagttg cgcaagccgg agccttacaa gggcaagggc
      181 gttcggtacc aaggtgaagt cgtgcgccgc aaggtcggaa aggctggtaa gtagtcatgc
      241 ccgttggatt gatcagccgc cgcaaggcgc gtggatccac caaggcggcg ctcatttcgc
      301 gccggcactt ccgcgtgcgc aagaaggtct ccggcactgc tctgcgaccc cggctcgtcg
      361 tgaaccgctc gacgcggcac gtgttcgctc aagtcgtcga cgacaccacc gggcgcacgc
      421 tcgcctcggc gtcgacgatg gaggccgacg tgcgcgcgac caagggcgac aagaccgcca
      481 aggcacgcaa ggttggcgaa ctgcttgcca agcgggctac cgctgcgggc gtcggacctg
      541 tcgtgttcga ccgtggcggc ttccagtacc acggccgcgt cgcggcgatc gccgacggcg
      601 cccgcgaagg tgggctcacg ctgtgaccgc gaagacgaat tgcaccaatc gcatcaaccg
      661 cgcgacgacg cacgtcgaga agaggaacgt ctgatgccag gacctcagcg ccgcggacca
      721 ggtggggccg cgggcgccgg cgcaggcgcc gccggggcaa gtgcgggcag tggcgacggg
      781 cgccgtgaca accgtcgtga caaccgccgt cccgaaggcg cagccgagaa gacccagtac
      841 atcgagcgcg tcgtagcgat caaccgcgtc gccaaggtcg tcaagggtgg ccgccggttc
      901 agcttcaccg cgctcgtcgt cgtcggcgac ggtgacggca gcgtcggcat cggatacggc
      961 aaggccaagg aagtaccggc cgcgatcgcc aagggtgtcg aggaagcgaa gaagagtttc
     1021 ttcaaggtgc cccggatcca acacacgatt ccgcacccga tcaccggcga agctgccgcg
     1081 ggtgtcgtgc tgttgcgccc cgcaagcccg ggtaccggcg ttatcgctgg tggtccggtg
     1141 cgtgcggtgc tcgagtgcgc cgggatccac gacgtgctgt cgaagtcgct tggctccgac
     1201 aacgcgatca acgtggtcca cgccaccgtg gcggcactga agggcctcat gcgtcccgaa
     1261 gaagtcgcgg cgcgccgcgg actgccgctc gaagacgtcg cgccggccgc gctgctgcgc
     1321 gcccgcgcgg ccgggatggg agcttgagat gggccgactg aaggtcaccc agacccgatc
     1381 tgagatcggc ggtacgagta ggcagcgcaa caccttgcgc actcttggtc tgaagcgcat
     1441 gcgcgacacg gtcgtcaaag aagaccgtcc cgagattcgc ggcatgatca aaacggtgac
     1501 gcatctcgtc gccgtcgagg aggtcgactg acgatggcag agacaacgag caagccgaaa
     1561 aaagctgcag ctaccgccga ggcggaagcc acgccacgcg ccgccggagt cttgaaggtt
     1621 caccacctgc ggccggcccc cggatcgcac aagccgaaga cccgcgtggg tcgaggcgaa
     1681 gcctcgaagg gcaagaccgc gggtcgcggg accaagggca ccaaggcgcg ctaccaggta
     1741 cccgagcgtt tcgagggcgg gcagatgccg ctacacatgc ggctgcccaa gctcaagggc
     1801 tttacgaacc gttttcgaac cgagtaccaa gtcgtgaacc tcgagtggat cgccgcgcac
     1861 tatcccaagg gcggcgccgt tactccagac gacctggtcg cgaagggtgc ggtgcgtaag
     1921 ggcgagctgg tcaaggtgct tggcgacggc gagatcgggg tcaagctcca ggtcagcgcg
     1981 cacgggttct cggcgtccgc gaaagacaag atcgaggctg ccggaggaag tgctaccaag
     2041 ctgtaagaaa gctccggtcc gcccgagccg gaccggtggg ttagagtcac ggagcgggcg
     2101 ccggtagctg gtttgcgttt ctcccgggca ccttcaggct cgtgcacgac agctggatgc
     2161 tcaacgcagg cgcgcaggag gaatcgtgaa ctacgcccta ctcagggcct tcgggcaggc
     2221 gttccgcacg cctgacctgc gcaagaagat gctggtcgtg ctcttcatcg tgctgctctt
     2281 ccgcttgggc agtgcgatcc ctgcgccggg tgtttccgag acgaacatcc acgcgtgtct
     2341 tgtccaggtc actggcggca acaacgccaa cgtctacacg ctgatcaact tgttcagcgg
     2401 tggcgcgttg ctcaagctgt cgatcttggc gctcggcatc atgccgtaca tcacgtcgag
     2461 catcatcatc cagctgctcg tcgtggtgat cccgcggctt gagcggttga agcaggaagg
     2521 tcagtcgggc ccggcgaagc tgacgcagta cacgcgttac ctgacgatcg gtctggccat
     2581 ccttcagtcg tccgcgttcg tcgcgcttgc gcgcacgccg ggccagatct tcactggctg
     2641 cagcacgccg atcattccca atgacggctg gcagg
//
LOCUS       DASWVF010000623         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_42660, whole genome shotgun sequence.
ACCESSION   DASWVF010000623 DASWVF010000000
VERSION     DASWVF010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42660"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>2373
                     /locus_tag="VGD55_10945"
     CDS             132..>2373
                     /locus_tag="VGD55_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870905.1"
                     /translation="MTSTNIDVFGNAYQSIDSGCIGGCARVDPVLTRYAQPAEVSGST
                     GWLWQNIDTYTSGTGDIPASTPRNRTEQTYDPEGSPLATSRVIQGSLPLARSNPGGGG
                     VAPAPPTASTDRTALLVTHAYDTFGNPITTVAAGGRCRATSYDASYAALPVNDTVDVG
                     TVPSGGSCGGTALVSSAAYDRGLGTITTLTDMRGETTNVQFDGLGRIAALFRPDPATG
                     IASAVPSMTLAYQLPSGPTTPYTLVHTEVQDGTTPTAQSYRESWTYVDGLGRTFVTLN
                     QADPASGDAGPWVVSGLTTFDGKGAPKLKYLDYFYSGDPTQFVASGSVGSAYSQQTYD
                     AFGRDLYTYALDRTAALANVYHALSVDAWDAADMTPTSGHYGTFATTQSDGHGRTIAV
                     TERAYTGKSPQAYVTTTAYESTGEPVLITRSSTSLPAAASVSRWMRYDSIGRMVANVE
                     PDTTVGYVADPAASVPAQTWRYAYDDEGDLVGTSDARGCGANFTYDTGGRIASEDYSP
                     CAATQPAYSTAPEVTYLYDAPDPAVTTGINGFAVTAGWYRGRLASTADRAGKTIVRYD
                     GRGRTTGVARQLVSPAGAVAPRWYVQESTFDADDRLTVEQTGAVDSGAALSITTQTTT
                     TFSKRSLTKSVVSNQWTVVASVAHAADKKPLQVVYGDAAGTTSLMSYDARRRLSSVQT
                     YRGPPAIWSSPPQTYVPPPSYAGPPSTFQLLLQDLAYTYDSVGNPLAIQDSRTPSEWP
                     SGAQPVS"
BASE COUNT          434 a          829 c          747 g          363 t
ORIGIN      
        1 cagccgacgt tcagctggag ctcacaccgc cgacgtattc cgccctggag gagaccatca
       61 tcccgcctgc ggcaccgaca cagcagtttg gcacgatgct cctgcaggga gccgcaggga
      121 tggcgcacct gatgacgtct acgaacatcg acgtcttcgg caacgcatat caatcgatcg
      181 atagcggctg catcggtggg tgcgcccgcg tcgatcccgt tctgacccgc tatgcgcaac
      241 ccgccgaagt gagcggaagc accggctggc tctggcagaa catcgataca tatacgagcg
      301 gaacggggga cattccggcg agcactccgc gaaatcgcac cgagcaaacg tacgaccccg
      361 aagggagccc gctcgcgacg tcgagggtaa ttcagggttc gcttccgctg gcacgatcca
      421 accccggagg cgggggagtt gcgcccgcgc cgccgaccgc gtccaccgac cgcacggcgc
      481 tcctcgtcac acacgcgtac gacacgttcg gcaatccgat cacgaccgtc gcggctggcg
      541 gccggtgtcg cgcgacttct tacgacgcga gttacgcggc gctccccgtc aacgacactg
      601 tcgacgtcgg cacagttcct tcgggtggaa gctgcggcgg aaccgcgctc gtttccagtg
      661 cggcatacga tcgggggctt gggacgatca cgacgctcac cgacatgcgg ggcgaaacga
      721 cgaacgtgca gttcgacggg ctcgggcgca tcgcggcgct gtttcggccg gatccggcga
      781 caggcattgc gtccgcagtc ccctccatga ctctcgcgta tcagctccca agtggcccga
      841 cgaccccgta cacgctcgtt cacaccgagg tccaggacgg caccaccccc accgcgcaga
      901 gctatcgaga gtcatggacc tacgtggacg gcctggggcg caccttcgtc acgctgaatc
      961 aggccgaccc cgcttccggc gacgccggcc catgggtcgt gtcagggctg acgacgttcg
     1021 atggcaaggg agcgccgaag ctgaagtacc tcgactactt ctattccggc gacccgacgc
     1081 agttcgtcgc gagcggctcc gtgggttcgg cgtactccca gcagacatac gacgccttcg
     1141 gtcgcgatct gtacacctac gcgctcgatc gcaccgccgc cctggcgaac gtctaccacg
     1201 cgctatcggt cgacgcgtgg gatgccgccg acatgacgcc aacgagcggg cactacggca
     1261 cgttcgccac gacacagagc gacggacatg ggcggaccat cgccgtcacg gagcgagcgt
     1321 acacaggaaa gagtccccaa gcgtacgtga cgacgaccgc gtacgaatcg accggcgaac
     1381 cagtgctcat cacgcggtcg agcacgagcc tacctgcggc ggcgtcggtg tcacgatgga
     1441 tgcgttacga ctcgatcgga cggatggtcg ccaacgtcga gccagatacg acggtcggct
     1501 acgtcgcaga tccggccgcc tccgttcccg cgcaaacgtg gcgctacgcg tacgacgacg
     1561 agggtgacct cgttggcacc agtgatgcgc gggggtgcgg cgcgaacttc acgtacgaca
     1621 cggggggccg gatcgcgagc gaggactact cgccgtgcgc cgccacacag cccgcctact
     1681 cgaccgcacc ggaggtgaca tacctctatg acgcgcccga tccggcggtc acgactggga
     1741 tcaacggctt tgccgtcacc gcgggctggt atcgcgggcg gcttgcatcg accgctgatc
     1801 gagccggcaa gacgatcgtg cgctacgacg gccgtggacg gacgacaggc gttgcgcggc
     1861 agctcgtctc ccctgcagga gcggtcgcgc cgcgctggta cgtgcaggag agcaccttcg
     1921 atgccgacga ccgcctgacg gtcgagcaga ccggtgccgt tgactccggg gccgccctct
     1981 cgatcacgac ccaaacgacg accacgtttt cgaagcgcag cttgaccaag agcgtcgtga
     2041 gcaaccagtg gaccgtggtt gcgtccgtgg cgcacgctgc cgacaagaag ccgctccagg
     2101 tcgtgtacgg cgacgcggca ggcaccacat ccttgatgag ctatgacgcc cgccgccgac
     2161 tgagcagcgt ccagacctac cgcggtcccc cggcgatctg gagctctcct ccgcagacct
     2221 atgtgccgcc gccctcctat gccgggccgc ccagcacctt ccagcttctg cttcaggatc
     2281 tcgcttacac ctacgatagc gtcggcaatc cgctcgccat ccaggactcg cgcacgccga
     2341 gtgaatggcc atccggcgcg cagccggtat cgc
//
LOCUS       DASWVF010000624         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_93788, whole genome shotgun sequence.
ACCESSION   DASWVF010000624 DASWVF010000000
VERSION     DASWVF010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93788"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..439
                     /locus_tag="VGD55_10950"
     CDS             92..439
                     /locus_tag="VGD55_10950"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0870906.1"
                     /translation="MLDETIDLDRTFHALADSSRRSMVERLTLGPASVSELAYPLAMS
                     LAAVVQHVQVLEACGVVTTAKVGRTRICRIEPVAMTAAERWITDRRQGWESRLDRLGD
                     VLNADAATREENP"
     gene            436..903
                     /locus_tag="VGD55_10955"
     CDS             436..903
                     /locus_tag="VGD55_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0870907.1"
                     /translation="MTLSTVTHDTVVIARTYDFPVATVFRAWADPRRKARWFAGSADA
                     LDNGYDLDFRVGGREVNRGGPPGGPVYTYSAEFRDIVTDQRIVSTSEMFADGSRISVT
                     VATVEFRESGGTTHLVVTEQAVFLDGHETAAQREEGTRSLLDSLAVSLAGGEP"
     gene            933..1499
                     /locus_tag="VGD55_10960"
     CDS             933..1499
                     /locus_tag="VGD55_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0870908.1"
                     /translation="MRTVASFIMISLDGFYEGRNGELDWPIIDQEFGDFALTQLDAAA
                     TLGFGRATYEHMAAYWPTAQATANDPAIASRMNDKDKLVFSTTLTEASWSGTTIVRGE
                     ATQHLPMIKAATDKELLVIGSAHLTANLAQAGVLDELRVMVCPITLGQGRSLFEDLNG
                     RVSLTLLRVKQFDSGNLVLTYRPSPHTN"
     gene            1854..2180
                     /locus_tag="VGD55_10965"
     CDS             1854..2180
                     /locus_tag="VGD55_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0870909.1"
                     /translation="MILLRRLLGDALRQQRLSQERTLRDVSAGARVSLGYLSEVERGQ
                     KEASSELLSSICDALGVQLSTVLREVSDELHVLESATAGPALVPVSGSAHGSTAHADH
                     QVVAAA"
     gene            2255..2929
                     /locus_tag="VGD55_10970"
     CDS             2255..2929
                     /locus_tag="VGD55_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0870910.1"
                     /translation="MAPPTADVIDEARNGGDSPSAHESASTRGTRSRAGNAMGRTHEA
                     ALRGALAAVAKYGSRKATMGDIALLAGVAKATLYNHFRTREDVYAAVAQAEVNAVAVA
                     ARAKLPEGFDAAVAEAGRLIADHPALRRVATDDPATLVAIATPGDGAAWAAARAHVTA
                     ALTQAGYAPTPAAVDVVLRYLTSQLLAPSSSVERTDAAALIMRAVSGQLQLPDSAPVG
                     SADTSS"
     gene            complement(2856..3671)
                     /locus_tag="VGD55_10975"
     CDS             complement(2856..3671)
                     /locus_tag="VGD55_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY0870911.1"
                     /translation="MPEGNTVWLAARTLRTALAARPLTIAELRVADLATTDLVGHHVT
                     DVVPRGKHILIRLDADLTLHSHLRMDGKWRIHPADQPVRDPHDHVRVVLGNAVHTAIG
                     DRIHDVALIPTAAEHTLVGHLGPDLLGPDWDAGEAVRRLLADPRRTIAEALLDQRNLA
                     GAGNIFKTETCFLRGVSPWTPVGDVRDLPGLVDLVHRLLLANRERYEHVTTGDARPGQ
                     RTWVYDRARRPCRRCGTEIQSAWQGDPGRLSYWCPRCQPGPSPEVVAGQKPRA"
     gene            complement(3687..>5639)
                     /locus_tag="VGD55_10980"
     CDS             complement(3687..>5639)
                     /locus_tag="VGD55_10980"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0870912.1"
                     /translation="VAAFIYEGDAPLAERRAQALALDSALLGELLGQAELREMLDADA
                     IDEIERELQHLTDERKARDLEGVADLLRLIGPLSTAEAVERGATPRWLAELEDARRAI
                     RIRITAEERWAAIEDAGRLRDALGSPLPVGIPDAFTESVNDPLGDLVSRYARGHGPFT
                     AADVAQRFGLGIAIVTGVLERLTATGRVVRGEFRPGGSGIEWCDAGVLRSIRRRSMAR
                     LRKEVEPVPAKAFGRFLPAWQHARNPLRGVDGVLRAVEQLQGVVLPASALEQLVLPCR
                     VADYSPAMLDELTVSGDVVWAGHGSLPGNDGWVALYPAESAPLLLPAREPNPTMSLLQ
                     RDLLAAMRGGGAMFFRELSNRVGASDDRELAAALWELVWSGLVTNDSLAPLRVLLGTG
                     RPRNTRSTTRRGRPLLPSRSGPPTVGGRWSLLPEPATDETRRSHALAETLLDRYGVLT
                     RGAVQAEGVPGGFAAVYRVLTAFEDAGRCRRGYFVEGLGAAQFASPGAVDRLRAPSGA
                     DAESPRAIVLAATDPANPYGAALPWPTRTVRAGSPLAAPPEREGHRPGRKAGALVVLV
                     DGELAVYVERGGKTLLSFEPESNEAAPDVIQTAVEALALAVRDGWLGRLDVERADGGS
                     VLQSPLGTALEAAGFRATPRGLRLRL"
BASE COUNT          973 a         1873 c         1871 g          922 t
ORIGIN      
        1 gcttgagggc ggtctcaggc cgagctctgg gccgcattct atagtcaagc atctacttga
       61 ctatagcgcg tgacctcgta cacttaagta catgcttgac gaaacgatcg acctcgatcg
      121 gaccttccac gcgctggctg atagcagcag gcgttccatg gtggaacgac tcaccctggg
      181 tcccgcttcg gtgagtgagc tggcgtaccc cctggctatg tccctcgcgg cggtggttca
      241 gcacgttcag gtgcttgaag cctgcggcgt cgtgacgacc gcgaaggtcg gcaggacgcg
      301 catctgccgg atcgagccgg tagcgatgac ggccgccgag cggtggatca ccgaccggcg
      361 tcaaggttgg gaatcccgac tggatcgact tggcgacgtg ctgaacgcag atgccgctac
      421 tcgcgaggag aacccgtgac cttgtccact gtcacccatg acaccgtcgt catcgcgcgc
      481 acgtacgact tcccagtcgc aacggtgttc cgagcgtggg cggatccgcg tcgcaaagct
      541 cgttggttcg ccggctctgc cgacgcactc gacaacgggt acgacctgga ctttcgggta
      601 ggagggcgcg aggtcaatcg cggcggacct ccgggtgggc ccgtgtacac ctactccgcg
      661 gagttccggg acatcgtgac tgatcagcgg attgtctcca cttcggagat gtttgccgac
      721 ggctcccgta tctcggtgac ggttgccacc gtggagttcc gcgagtccgg cggcacaacg
      781 cacctcgtcg tcacggagca agcagtgttc ctcgacggcc acgagacagc ggctcaacgc
      841 gaggaaggga cccggtcctt gctcgattcg ttggcggtct cgctcgccgg aggcgagccg
      901 tgatacggcg gttcacccac aaggaccatc caatgcgaac cgtggcatcg ttcatcatga
      961 tcagcctcga cggtttctac gaaggccgaa acggcgaact cgactggccg atcatcgatc
     1021 aggagttcgg cgatttcgcc ttaacacagc tcgacgcggc agccacgctc ggcttcggac
     1081 gcgcgaccta tgaacacatg gccgcctact ggccgacagc acaggccaca gcaaacgacc
     1141 cggccatcgc gtcacggatg aacgacaaag acaagctcgt cttctcgacg acgctcaccg
     1201 aagcgagctg gtcaggaacc accatcgtcc gcggcgaagc gacacagcac ctaccgatga
     1261 tcaaggctgc tacggacaaa gaactgctcg tgatcgggag cgcgcatctc accgccaacc
     1321 tcgctcaagc cggcgttctc gatgaactgc gtgtcatggt ctgcccgatc accctcggcc
     1381 agggccgatc actcttcgag gatctgaacg gccgtgtctc actcacgctc ctccgagtca
     1441 agcagttcga ctccggcaac ctcgtgctca cctaccgacc gtcacctcac acgaactgag
     1501 ccaggagaca gcaggccgaa gaaccgtgcc gccgtgctcc gtttcgtacc gaacatcgac
     1561 aggttcagcc gaggcccccg cgccctaacc ggcactacgg ctgatcctgg ttgaggtgac
     1621 gtctctaggt agatcggctg gcgaccccgg tcgtggttca gacctcgcat atccaggtgc
     1681 cggcaccgcg agataggtat gcttacactg ttacatcaca agacccgaac ggataagtca
     1741 caggccggga tcgggtgtca agatcagtcg agttgctcgc tcagcatgcc gatgacacaa
     1801 ggtacggtgg tcgtgatgcc ggcagcggcc aggctgaggg cgaggagggc acaatgatcc
     1861 tgctgcgcag gttgcttggc gacgcattgc gccaacagcg cctctcacaa gaacgcacgc
     1921 tgcgagatgt atccgccggt gcgcgggtct ccttgggcta tctgtccgaa gtcgagcgcg
     1981 gccagaagga agcgtcgtct gagttgctgt cgtcgatctg cgatgcactc ggcgtccagc
     2041 tttcgaccgt cttgcgcgag gtcagcgacg agttgcacgt gctcgaatcg gccaccgccg
     2101 gtcctgctct tgttcccgtg tccggcagcg cgcacggcag cacggcccat gccgaccacc
     2161 aggtcgtcgc agccgcctga cctgcaccag cccgccgtcc ccacccgcgt tcgtttcctt
     2221 cggtgtcggc cgtgcgtgcg cctagactgc cgctgtggca cctccgacgg cagacgtgat
     2281 cgacgaagcc cgtaatggcg gcgattcacc gagcgcgcac gaatccgcga gcacgcgggg
     2341 cacgcgaagt cgcgctggga acgcgatggg tcgcacccac gaagctgccc tgcgcggggc
     2401 attggccgcc gtcgctaagt acggcagccg caaggccact atgggcgaca tcgccttgct
     2461 cgccggtgtc gccaaagcca cgctgtacaa ccacttccgc acccgcgagg acgtctacgc
     2521 cgcggtcgca caagccgagg tcaatgcggt cgcggttgca gcacgtgcca agctccccga
     2581 aggcttcgac gcggcggtcg cggaagcagg tcggcttatc gctgaccacc ctgcattgcg
     2641 gcgggttgcg accgacgacc ccgcgaccct cgttgccatc gcaaccccag gagacggtgc
     2701 cgcgtgggcc gcggcgcgcg cgcacgtgac ggccgcgctc acacaggcgg gttacgcacc
     2761 aacgccagcc gctgttgacg ttgtgctgcg ttacctcacg agtcagctgt tggcgccatc
     2821 gtcgtcggtt gagcgaacgg acgccgcagc gctgatcatg cgcgcggttt ctggccagct
     2881 acaacttccg gactcggccc cggttggcag cgcggacacc agtagctgag cctgccgggg
     2941 tcgccctgcc atgcgctttg aatctctgtc ccgcatcgcc ggcacggtcg acgtgcgcgg
     3001 tcgtacaccc acgtgcgttg gccgggtcgc gcgtcgcccg tcgtgacgtg ctcgtaccgc
     3061 tcgcggttcg ccagcaggag tcgatggacg agatcgacaa ggccagggag atcacggacg
     3121 tcgcccaccg gcgtccatgg tgaaacgcct cgcaggaagc acgtttcggt cttgaagatg
     3181 ttgccggcgc cggcaaggtt gcgctggtct agcagcgctt cggcgatggt gcgtctcgga
     3241 tccgcgagca gtcggcggac cgcttcgccg gcatcccagt cggggccgag caggtcgggc
     3301 ccgaggtgcc cgacgagggt gtgttcggcg gcggtgggga tcagtgcgac gtcgtgaatg
     3361 cggtcaccga ttgcggtgtg gaccgcatta ccgagcacga cgcgaacgtg gtcgtgcgga
     3421 tcgcgtaccg gttggtcggc agggtgaatg cgccacttgc cgtccatgcg caggtggctg
     3481 tgcagcgtca ggtcagcgtc gaggcggatc agaatgtgct tgccgcgcgg cacaacgtca
     3541 gtgacgtgat gcccgacgag gtcggtggtc gcgaggtcgg cgactcgcaa ctctgcgatg
     3601 gtcagcgggc gcgcggcaag tgcggtccgc aacgtgcggg ccgcaagcca gacggtgttg
     3661 ccctcaggca tcgcacatcg tgggcgtcac aaccgcaggc gcagacccct cggggtcgcg
     3721 cggaacccgg ctgcctccaa cgcggtgccg agcggtgact gcaacaccga cccgccgtcg
     3781 gcacgttcga catcgagccg cccgagccag ccgtcgcgaa cggcgagggc aagcgcctcg
     3841 accgccgtct ggatcacatc gggtgcggcc tcgttgctct cgggctcgaa cgacagcagc
     3901 gtcttgccgc cgcgctcgac atagaccgca agttcgccat cgaccaggac gacgagggca
     3961 cccgccttgc ggcccggccg atgtccttcc cgctcgggcg gggcagccag gggactgccc
     4021 gcgcgaacgg ttcgggtcgg ccagggcagt gccgcgccgt agggattcgc cggatcggtg
     4081 gccgccagca cgatcgcgcg cgggctctcg gcgtcggcgc ctgagggtgc tcgtaggcgg
     4141 tcaaccgcgc caggcgaggc gaactgagcc gccccgagac cttctacgaa gtaaccgcgt
     4201 cggcagcggc cggcgtcctc gaaggcggtc agcactcggt agaccgcggc gaacccgccg
     4261 ggcacgcctt cggcctgaac agccccgcga gtgagcacgc cgtaacggtc gagcaacgtt
     4321 tcggcgagcg cgtgcgagcg ccgcgtctcg tcggtcgcag gttcgggcag caggctccat
     4381 cggccgccga ctgtcggagg gccagagcgg cttggcaaca gtggtcgacc gcgtcgagtg
     4441 gtcgaccggg tgttgcgcgg tcgaccagta ccgagtagca cgcgcagtgg cgcgagcgag
     4501 tcgttcgtca cgaggcccga ccacacaagc tcccacaggg cggcagcgag ttcgcgatca
     4561 tcggatgcgc cgacgcggtt cgacagctcg cggaagaaca tcgccccgcc gccgcgcatg
     4621 gcggcgagaa ggtcgcgctg aagtaatgac atcgtcgggt tcggttcgcg ggccggcagg
     4681 agcagaggcg ccgactccgc gggatacagc gcgacccacc catcgttgcc gggcaaggat
     4741 ccgtggccgg cccacacgac gtcaccactg acggtgagct cgtcgagcat cgcgggcgaa
     4801 tagtcggcga cacggcaagg caaaaccagc tgctcgagcg ccgacgctgg caacaccaca
     4861 ccctggagtt gttcgaccgc gcggagcaca ccgtcgacac cgcgcagtgg attgcgcgca
     4921 tgctgccacg cggggaggaa ccggccgaag gctttcgccg gtaccggctc gacctctttg
     4981 cgtagccgtg ccatcgaccg gcgccggatc gaccgcaaga cgcccgcatc acaccattcg
     5041 atgcccgaac cgccaggccg gaactcgccg cggacaacgc gacccgttgc ggtcagtcgt
     5101 tcgaggacgc cagtgacgat cgcgatgccg agcccgaatc gctgcgcgac atcagccgcc
     5161 gtgaacgggc cgtggcctcg cgcgtagcgc gagacaagat cgccgagcgg atcgttgacc
     5221 gattcggtga aggcgtcagg aatccctaca ggcaacggac ttccgagcgc atcgcgcagt
     5281 ctcccggcgt cttcgattgc cgcccatcgc tcttcggcgg tgatgcggat tctgatggcg
     5341 cgtcgggcgt cttcaagctc agcgagccag cgtggcgtag caccccgctc gacggcttcg
     5401 gccgtagaaa ggggcccgat gagccggagc agatcggcaa caccttctag atcgcgtgct
     5461 ttccgttcgt cggtgaggtg ctgcagttcg cgttcgatct cgtcgatcgc gtcggcgtcg
     5521 agcatctcgc gcagctcggc ctggccgagc aattccccga gcagtgcgct gtcgagggcg
     5581 agtgcttggg ctcggcgttc ggcgagtggc gcgtcgccct cgtagatgaa tgcggcaac
//
LOCUS       DASWVF010000625         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_374036, whole genome shotgun sequence.
ACCESSION   DASWVF010000625 DASWVF010000000
VERSION     DASWVF010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374036"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGD55_10985"
     CDS             <1..348
                     /locus_tag="VGD55_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870913.1"
                     /translation="WRVACGISTVLFLTAAIIQLASMTNLGWAPGSSGFASVYVATMP
                     LFAIYCLATAYWLETLLARSLRVRWVLSPEGTNRDDRIQIAFAGSAVGAKAFVVFVAL
                     VNIVLFVLFSVLN"
     gene            complement(465..1151)
                     /locus_tag="VGD55_10990"
     CDS             complement(465..1151)
                     /locus_tag="VGD55_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870914.1"
                     /translation="MTDSPTQADATDADAQLQPVAPADPQTPPLWSWVLRHRKSSIAG
                     TVVIVLALVAVVDWPHRATPGQLRSDFAQYATQVRGDLQSCSVEVTETLSAYNQITAG
                     VVRDRQTAQAIASQTALDCTPMGNSRIDDLGTLQPPSSLANYGLGAATQQLYVWTFPA
                     AVDVAQDIERLMSSPGDPATLADVKAKLTQMNTVGTSAQNAFNKAAGSMATPPVDFAL
                     DQVRPSVLVG"
     gene            1247..2119
                     /locus_tag="VGD55_10995"
     CDS             1247..2119
                     /locus_tag="VGD55_10995"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0870915.1"
                     /translation="MIAVIAAVHGRGLRRHLNAIARSGRPTGPWLGQAFIFWAALVVL
                     LLAVVSPVDYWSDTYLSAHVVQHILLAFVAPPLLVLGAPWVPLLRGLPRPVARVYGAL
                     LRRTRGRRPVSGASGWRAAAAVRKVASRPWTPVVLFNVNMLFWHFPGPFDLGARNSSV
                     HIWLEHGSFFGLGVCLWLQIFGSYPFRPALPPVGRVVALIASNVTMVVIAMTMVMFTH
                     DLYPWYASRGLAAQESDQQIAGAILWVCGEVTFLPSILYTITKWLDDGADRSTRRAVA
                     GSPVANHRGAQMLL"
     gene            complement(2151..>3293)
                     /locus_tag="VGD55_11000"
     CDS             complement(2151..>3293)
                     /locus_tag="VGD55_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870916.1"
                     /translation="GRRGVAAAFTAGVVTAGALTACGSHSTSASDTAGAISVPLATSV
                     VTSSATWATFAMGHLDDPENTFWEEFTLPTGADQWVERTPVDVADNGGLVTAPTSDGV
                     VVGFRPSADLKFSPLASTSDDGATYTPGLLSDGLANVPDALSVTASGHAAALTSTEVM
                     TSGAMLSAWEPVTTLSAISTSSVGQSCGVQELTGVLVTENGLFVGAACSSPGVVGLLQ
                     HSGSAFVSAGLRLPSGDAQARISVLRLVQYKGTIAVLLGVRTDASTSYVAAWNPAPGS
                     AAWTVSPPLTLPGALVSTAVTGDGSYAMMTSDNSGLRAALIAERGTSWTQLPPPPAGT
                     ATLAVSKARTDALVVDSATFIDYQLTDGQWVKGQTTQVAIPYGSSG"
BASE COUNT          493 a         1053 c         1111 g          636 t
ORIGIN      
        1 tggcgagtgg cgtgcggcat ctccacggtg ctgttcttga cagccgcgat catccagctt
       61 gcgagcatga cgaacctggg ctgggcgccg ggctcgtcgg gcttcgcgtc ggtctacgta
      121 gcgacgatgc cgctgtttgc gatctactgc ctcgccaccg cgtactggct cgagacgttg
      181 ctggcacggt cgttgcgggt gcggtgggtg ctctcgcccg aggggacgaa tcgcgacgat
      241 cgcatccaga tcgcgtttgc cggctcggcc gttggcgcga aggccttcgt ggtcttcgtg
      301 gcgctggtca atatcgtgct cttcgtcctc ttctccgtgc tgaactgaga ggtagcgatg
      361 cacaccagcc agttcatgac gttcatcttt ccggtgtcac tgtttgtggc cgcggtggtg
      421 tggggcttct tccaacgccg ggggaactag ctctgctcac gcggttaccc gacgagcacg
      481 ctcggccgca cctgatcgag tgcgaagtca acgggcggtg tcgccatgct ccctgccgct
      541 ttgttgaacg cgttctgcgc gctggtgcca acggtattca tctgggtgag cttggccttg
      601 acgtcggcca gcgtggccgg gtcgcccggc gacgacatga ggcgctcgat gtcctgggcg
      661 acatcgacgg cggccgggaa ggtccacacg tacagctgct gcgtcgcggc gccgaggccg
      721 taattcgcca gcgacgaagg cggctgcaag gttccgaggt cgtcgatgcg gctgtttccc
      781 atcggcgtgc agtcgagcgc ggtctggctg gcgatcgcct gagccgtctg ccggtcgcgc
      841 accacgcctg cggtgatctg gttatacgcg ctgagcgtct ctgtgacctc gacgctgcac
      901 gactggagat cgcctctgac ttgggtcgcg tactgagcga agtcagatcg cagttggcct
      961 ggagttgcgc gatgcggcca gtcgactaca gcgacaagag cgagcacgat gacaaccgtg
     1021 cctgcgatgc tgctcttgcg atggcgcaga acccacgacc acagcggcgg tgtctgtgga
     1081 tcagccgggg cgaccggctg cagctgcgcg tcggcgtccg tcgcgtcagc ctgagtcggg
     1141 gagtctgtca ccgctggaac actacagcca caacatgtgt cgcaactgag gaaggaacgc
     1201 ttcgatagcc gtcttccagc actggtcctt cgacccgttc attgtggtga tcgcggtcat
     1261 cgctgctgtc cacgggcgcg ggttgcggcg gcacctgaac gcgatcgcga ggtcgggccg
     1321 cccgaccggt ccgtggctcg ggcaagcgtt catattttgg gctgccctcg tcgtcctgct
     1381 gctggctgtt gtatcgcccg tcgactattg gtccgacacc tatctgagtg cgcacgtggt
     1441 gcaacacatc ttgctggcgt tcgttgcgcc gccgctgctc gtgctcggag cgccgtgggt
     1501 tccgctgttg cgtgggttgc cgcggccggt cgcacgcgtg tatggcgcgc tgctacgacg
     1561 gacgcgtggt cggcgtccgg tttctggtgc gtcggggtgg cgcgccgcgg ccgctgtgcg
     1621 caaggttgcg tcgcggccgt ggacgccggt cgtgctgttc aacgtcaaca tgctgttctg
     1681 gcacttcccc ggaccgttcg acctcggcgc gcggaactcc agcgtgcaca tctggctcga
     1741 gcacggatcg ttcttcggac tcggcgtgtg cctgtggctc cagatcttcg gctcgtaccc
     1801 gttccggccg gcgcttccgc ccgtcgggcg cgtcgtcgcg ctcatcgcgt cgaacgtcac
     1861 gatggtggtg atcgcgatga cgatggtgat gttcacccac gacctctacc cctggtacgc
     1921 gagccgcggt ttggccgccc aggagagcga tcagcagatc gccggtgcga ttctgtgggt
     1981 gtgcggcgag gtgacgttcc taccgtcgat cctctacacg atcacgaaat ggctcgacga
     2041 cggagctgat cgttcgacgc gccgcgccgt cgcggggtcg ccggttgcca atcatcgtgg
     2101 agcccagatg ttgctctagc acccgccagg ctccaccttg ttgtggagag tcagccgctt
     2161 gagccgtacg ggatcgcaac ctgcgtcgtc tgccccttga cccactgccc gtcggtcagt
     2221 tggtagtcga tgaaggttgc gctgtcgacc acgagtgcgt cggtgcgcgc cttcgaaacg
     2281 gccagcgtgg cggtacccgc gggaggcggc gggagttggg tccaactcgt gccccgctcg
     2341 gcgatcagcg cggctctcaa cccgctgttg tcggacgtca tcatcgcgta gctgccgtcg
     2401 ccggtgaccg cagtcgagac gagcgcgccg ggcaacgtca gcggcggcga tacggtccaa
     2461 gccgccgagc ccggtgcagg attccacgct gcgacataac tcgtcgaagc atcagtgcgg
     2521 acgccgagga gaaccgcaat tgttcccttg tactgaacca ggcgcagcac cgaaatccga
     2581 gcctgcgcat caccgctcgg caatcgcagg ccggccgaaa cgaacgccga cccggagtgc
     2641 tggagaagtc cgaccacgcc aggcgacgaa catgcagcgc cgacgaacag tccgttctca
     2701 gtaacgagca cgccggtcag ctcttgcacg ccacacgatt gcccgaccga ggacgtgctg
     2761 atcgcggaga gggtcgtcac cggctcccag gccgacagca tcgcgccgct cgtcatcacc
     2821 tctgtcgacg tcagtgcggc ggcgtggccg ctcgccgtca cgctgagcgc gtcgggcacg
     2881 ttagccaagc cgtcgctcaa caggcccggt gtgtaggtcg cgccatcgtc cgatgtcgat
     2941 gcgagcggcg agaactttag atcggcgctg ggcctaaagc cgacgacgac gccgtcggag
     3001 gtcggtgccg tcacgagccc gccattgtcg gcaacgtcga caggtgtgcg ttcgacccat
     3061 tggtcggcgc cggttggcag cgtgaactct tcccagaacg tgttctcagg atcgtcgagg
     3121 tgacccatcg cgaacgttgc ccacgtggca gaagacgtga cgaccgatgt cgcgagcgga
     3181 acgcttatcg cgcctgcggt atctgaagcg gacgtcgagt gcgagccgca ggcggtcaac
     3241 gcgcccgccg ttacgacgcc ggctgtaaag gcggcggcaa cacctcggcg tcc
//
LOCUS       DASWVF010000626         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_93855, whole genome shotgun sequence.
ACCESSION   DASWVF010000626 DASWVF010000000
VERSION     DASWVF010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93855"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="rplX"
                     /locus_tag="VGD55_11005"
     CDS             <1..300
                     /gene="rplX"
                     /locus_tag="VGD55_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0870917.1"
                     /translation="ISGKDRGFKGRVIETDPGRERVLVEGVNRIRKHTKVTSTTRGAK
                     TGGIVTQEAPVHVSNVMVVCPECDKATRIGRRQVLNENTGKTIPVRICKKCGKEI"
     gene            311..892
                     /gene="rplE"
                     /locus_tag="VGD55_11010"
     CDS             311..892
                     /gene="rplE"
                     /locus_tag="VGD55_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0870918.1"
                     /translation="MTTTTIEAPKASTIPRLKQRYRDEIAGQLREQFGYENVMQIPGL
                     TKVVVNMGVGEAARDAKLIDGAVRDLTTITGQKPQVTRSKKSIAQFKLRENMAIGCHV
                     TLRGDRMWEFLDRLLALALPRIRDFRGLSPRQFDGHGNYTFGLTEQVMFHEIDQDKID
                     RFRGMDITVVTTAKTDDEGRAFLRLLGFPFKEN"
     gene            892..1077
                     /locus_tag="VGD55_11015"
     CDS             892..1077
                     /locus_tag="VGD55_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY0870919.1"
                     /translation="MAKNALKIKAAAKPKFKVRGYTRCNRCGRSHSVYKKFGICRICL
                     REMAHRGELPGITKSSW"
     gene            1172..1579
                     /gene="rpsH"
                     /locus_tag="VGD55_11020"
     CDS             1172..1579
                     /gene="rpsH"
                     /locus_tag="VGD55_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0870920.1"
                     /translation="MMMTDPIADMLTRVRNANHAYHDNVPIPYSKIKTHVADILKEQG
                     YIAGWHVEDVEGHVAAKTLVVELKYGPNRERSIAGVRRVSKPGLRVYAKGTNLPRVLG
                     GLGIAIISTSGGLLTDKQCAKRGVGGEVLAYVW"
     gene            1597..>1938
                     /gene="rplF"
                     /locus_tag="VGD55_11025"
     CDS             1597..>1938
                     /gene="rplF"
                     /locus_tag="VGD55_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0870921.1"
                     /translation="MSRIGRLPIPVPSGVDVDITDRVVTVKGPKGTLTHTLADPILIE
                     KGDDGALHVTRPDDEKRSKERHGLSRTLVANMVTGVTDGFSKTLEIVGTGYRVQAKGS
                     DLEFALGFSHPV"
BASE COUNT          410 a          610 c          612 g          306 t
ORIGIN      
        1 atctccggca aggaccgcgg tttcaagggc agggtcatcg agaccgaccc tggtcgcgaa
       61 cgcgtccttg tcgagggcgt caaccgcatt cgcaagcaca cgaaggtcac ctcgaccacc
      121 cgtggcgcga agaccggtgg catcgtcacg caggaagcgc cggtgcacgt cagcaacgtc
      181 atggtcgtgt gccccgaatg tgacaaggcg acgcgcatcg gacgtcggca ggtgctgaac
      241 gagaacaccg gcaagacgat tccggtgcgg atctgcaaaa aatgcggcaa ggaaatctga
      301 ggatcacgac atgacgacca cgaccatcga agcgcccaag gcgagcacga ttccgcgcct
      361 caagcagcgc taccgcgacg agatcgccgg gcagctgcgt gagcagttcg gctacgagaa
      421 cgtcatgcag atccctggcc tcaccaaagt tgtcgtcaac atgggtgtcg gcgaggctgc
      481 tcgtgacgcc aagttgatcg acggcgcggt gcgcgacctg acgacgatca ccggccagaa
      541 gccgcaggtg acccgctcga agaagtcgat cgcccagttc aagctgcgcg agaacatggc
      601 gatcggctgc cacgtcacac tgcgcggcga ccgcatgtgg gagttcctcg accggttgct
      661 cgctctcgcg ctgccccgta tccgtgactt ccgcggcttg tcgccgcgac agttcgatgg
      721 ccacggcaac tacacattcg gtctcaccga gcaggtcatg ttccacgaga tcgaccagga
      781 caagatcgat cgcttccgcg gcatggacat cacggtggtc acgaccgcga agaccgacga
      841 cgaggggcgc gcctttcttc gcctgctcgg cttccccttc aaggagaact gatggcgaag
      901 aacgcactga agatcaaggc ggccgcgaag ccgaagttca aggtgcgcgg gtacacgcgc
      961 tgcaaccggt gcggccggtc gcactcggtg tacaagaagt tcggcatctg ccgcatctgc
     1021 cttcgcgaga tggcgcaccg cggcgaactg cccggcatca cgaagagcag ttggtagtga
     1081 gctgcgtagc tggtaaccct ctatacgccg caggtcgtca cggtgaatcc acgacgaggc
     1141 gaaaccacgg cgagaaaggc catgtggcca catcatgatg accgacccga tcgcggacat
     1201 gttgacgcga gtgcgcaatg cgaaccacgc gtaccacgac aacgtgccga ttccgtattc
     1261 caagatcaag acgcacgtcg ccgacatcct caaagagcag ggctacatcg ccggctggca
     1321 cgtcgaggac gtcgaaggcc acgttgcggc gaagactctg gtcgtcgagc tgaagtacgg
     1381 cccgaaccgc gagcgttcga tcgcgggcgt ccgccgcgtc agcaagcctg gtctgcgcgt
     1441 ctatgcgaag ggcaccaacc tgccgcgggt cctgggcggc cttggcattg ccatcatctc
     1501 gacatcaggc gggctgctga ccgacaagca gtgcgcaaag cgtggggtag gcggggaagt
     1561 cctcgcctac gtctggtaac aggaagagac gaactcatgt cccgtatcgg aagacttccg
     1621 atccctgttc ccagcggggt cgacgtcgac atcaccgacc gcgtggtcac ggtcaaaggc
     1681 ccgaagggca cgctgacgca tacgcttgcc gacccgatcc tcatcgagaa gggtgacgac
     1741 ggtgccttgc acgtcacgcg ccctgacgac gagaagcgga gcaaggagcg gcacggcctg
     1801 agccgcacgc tggtcgccaa catggtgacc ggggtaaccg acggtttctc gaagaccctc
     1861 gagatcgtcg gtaccggcta tcgcgtgcag gccaagggct cggatctgga attcgctctc
     1921 gggttctcac accccgtc
//
LOCUS       DASWVF010000627         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_17531, whole genome shotgun sequence.
ACCESSION   DASWVF010000627 DASWVF010000000
VERSION     DASWVF010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17531"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGD55_11030"
     CDS             <1..364
                     /locus_tag="VGD55_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870922.1"
                     /translation="QPVLFTARAVILVHARGVQGYGWGALTGAGWPQLVVHVGGIEAT
                     IGPFDGLVSGNTMLTEGASMRLDRLSAFPIVAGSRDCIRLVGNDGTGAREWKVSPRSS
                     SIDELWTQLLAAGVTPSD"
     gene            complement(541..1113)
                     /locus_tag="VGD55_11035"
     CDS             complement(541..1113)
                     /locus_tag="VGD55_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870923.1"
                     /translation="MPTCSDADPLPHLPNRVLVAGASGAGKTTLARRIPKALGIAHVE
                     IDALFHGPGWVTRPSFREDVDQFTEQPQWVTEWQYDEVRALLVARADTLVWLDLPRRT
                     VMARVTSRTIGRRLRREQLWNGNTEPHLVTVFTDRDHIIRWAWRTYRRTEYLVAQASI
                     QNPSLTVVRLRTQHEVDSWIAGPLANVAKS"
     gene            1222..1638
                     /locus_tag="VGD55_11040"
     CDS             1222..1638
                     /locus_tag="VGD55_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870924.1"
                     /translation="MAACTLHEIETAIRRAWSLDTCDPVDVPEWSAANPARGQCGATA
                     LVLHDLLGGDLLRAEVRYADGSRQGHHYWNLLTGGVEIDLTAEQFGAGEVIQAPVVVA
                     RPDGPPRRCAEQYLRLRSLVLAELHVQPHPRRVVRR"
     gene            1799..2437
                     /locus_tag="VGD55_11045"
     CDS             1799..2437
                     /locus_tag="VGD55_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0870925.1"
                     /translation="MKKYLLFDHDGVLVDTEFWYYTAGQRALADLGVSLDKEQYLRDM
                     SQGLGTWAQGRQAGIDEETISRQRKVRDVYYREYLRTEAIEIEGVVDILAELSNFVRM
                     AIVTTAKRVDFEIIHEKRQIRQFMDFVLVREDYELAKPHPEPYLTGLRRFGAAKEETL
                     VVEDSSRGLNSAVAAGIECVVVDNDFTKAQDFSQATYRINTLSELKDILAFT"
     gene            complement(2544..2774)
                     /locus_tag="VGD55_11050"
     CDS             complement(2544..2774)
                     /locus_tag="VGD55_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY0870926.1"
                     /translation="MARGVVKFWNAEKGWGAISSEELPAGHDAWAHFSQIMDMPGYRT
                     LEAGQLVEFSYESARQDSFNFRAIQIRLVAGD"
     gene            3263..3601
                     /locus_tag="VGD55_11055"
     CDS             3263..3601
                     /locus_tag="VGD55_11055"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0870927.1"
                     /translation="MTCVVAVPATGDPFEALGDPNRREILRLLSSGDKPVQEIAAALP
                     ISRPAVSRHLRLLKDAGLVAERPVGTRRIYHLQAEGVHAVQAYLEGIWGDAASRFRLL
                     AENTERSPQQ"
     gene            3598..4062
                     /locus_tag="VGD55_11060"
     CDS             3598..4062
                     /locus_tag="VGD55_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0870928.1"
                     /translation="MTDPLRFSFDVACSVEHAFAVWTSGIDAWWPRDHTVTADPGLVV
                     VLQGVVGGRIFERTPDGAEHDWGEVTAWEPPTLLAYRWHLGQDLADATDVEVRFVAQG
                     AAATRIDVEHRGWDRLGARGEVLRGRNRLGWDAFLSHYAPATAVPATAEGVQ"
     gene            4062..4466
                     /locus_tag="VGD55_11065"
     CDS             4062..4466
                     /locus_tag="VGD55_11065"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870929.1"
                     /translation="MAEGSKSDPWVLDTPPGTSEYTMYKDEEADPPALVCQVGSTTLK
                     YQLRAIDDLAAWLRKQGDWVPLGAADEQKPAAEGTVEAWGRADDNPVGGWYGLRKGYR
                     GRFGMYMPPLLEALGLAELTHDPRNNRMRATS"
     gene            4463..4765
                     /locus_tag="VGD55_11070"
     CDS             4463..4765
                     /locus_tag="VGD55_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870930.1"
                     /translation="MSTTRVIRYTTHPEHADENARLIGDVFSALAGKMPAGLHYAALR
                     LEDGVSFMHIVTLDGEENPLNDVPAFAEFQSNIMNRVVAPPVTVFATVVGNYELLV"
     gene            complement(4762..>5260)
                     /locus_tag="VGD55_11075"
     CDS             complement(4762..>5260)
                     /locus_tag="VGD55_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870931.1"
                     /translation="VLLAFAIDFEHDSTVSLAISANVLRVLTDGGVRVRDLPMLTGIS
                     KEAAAMSAKYLEAVGCATIEPVPAPDRGQCIRLTAKGRAAQEYAGKLSSTIETDWQTR
                     FGTDAVRSIRKSLEPLVGGGTVDSPLFAGLEPYPDGWRAAVPKPATLPHFPVVLHRGG
                     FPDGS"
BASE COUNT          920 a         1593 c         1755 g          992 t
ORIGIN      
        1 tcaaccggtg ctcttcaccg ccagagctgt gattctcgtg cacgcgcgtg gcgtgcaggg
       61 ttacggctgg ggagctctca ccggtgcagg atggccacag ctcgtggtgc atgtaggcgg
      121 gatcgaagcg accatcggac cgttcgatgg gctggtgtca ggtaacacaa tgctgaccga
      181 gggcgccagc atgcgcctgg atcgtctgtc ggcatttccg atagttgccg gcagtcgcga
      241 ttgcatcagg ctcgtcggca acgacggcac cggggcccgc gaatggaagg tgtcgccgcg
      301 gagtagctcg atcgatgaat tgtggactca gctgcttgcc gccggggtta ctccgagtga
      361 ctaggtcccg tttctgagat gcgttttcgc gttgggtgcg gggtgatttc gtcggcggtt
      421 ttggttccca gaatcggtag gcgcgacgtt ccacgcgcac acaactgccg ctgtgttggt
      481 cgatcgcgcc gagttgggag tgcctgcacg gcacctgtcg ggtcacgctt gttggtccgg
      541 tcagctcttg gcgacgttcg ctagggggcc ggcgatccag ctgtcgacct cgtgttgcgt
      601 tctgaggcgc acgacggtca ggctcgggtt ctggatcgac gcctgcgcta ccaggtactc
      661 ggttcggcgg tatgtcctcc aggcccagcg gatgatgtgg tcgcggtcgg tgaagacggt
      721 caccaggtgg ggttcagtgt tgccgttcca cagttgctca cgacgcagtc gtcgaccaat
      781 ggtgcggctg gtcacccggg ccatcactgt gcgcctcggt aggtcgagcc aaaccagtgt
      841 gtctgctcgg gcgaccagca atgcgcgcac ctcgtcgtat tgccattcgg tcacccactg
      901 cggctgctcg gtgaactggt cgacgtcctc cctgaagcta ggccgcgtga cccatccggg
      961 tccgtggaac agggcgtcga tctccacatg ggcgatccca agggctttgg ggattcgacg
     1021 cgcgagggtg gtcttgccgg cgccgctggc cccggccacc aagacgcggt tgggtaaatg
     1081 gggcagaggg tcggcgtcgc tgcatgtcgg cacgggtcga cagtagtcac ggccacacga
     1141 agccgtgacg atgtgtcagc gaagacgacc gatcgcccta cagggtggcg gcgcgcgcgc
     1201 ggatgcgggc acgataggtc ggtggctgcg tgcactctcc atgagatcga gacggcgatc
     1261 cgtcgagcct ggtcgcttga tacctgcgac cccgtcgatg tgcccgaatg gtcggcggcc
     1321 aatcccgctc gaggccaatg tggtgcgacc gctctcgtgc tgcacgatct cctcggcggt
     1381 gatctgctgc gcgcggaggt gcggtacgcc gatggttcgc gtcaaggtca ccactactgg
     1441 aacctgctca ccggcggggt tgagatcgac ctgaccgcgg agcagttcgg cgccggcgag
     1501 gtcatccaag cgcctgttgt cgtggcccgt cccgatgggc caccgcgacg ttgcgcagaa
     1561 cagtacctgc gtcttcggag cctggtgctg gccgagctac atgtccaacc tcacccacga
     1621 cgagttgttc gccgctaagg cctcgccgac tccccacgca ctattccgct gtgacccgcg
     1681 catctctcac tccgatttgc ggacgtcgaa gaagtaccca ggtctgccgt gcgatgacac
     1741 ctcacctgct gacacagtga ccctgttgac agcctgcggc acggaaagga tcgacagagt
     1801 gaagaagtac ctcctcttcg atcatgacgg cgttcttgtg gataccgagt tctggtacta
     1861 cacggctgga cagcgcgccc tagcagattt gggtgtgtcg ttggacaagg agcaatacct
     1921 gcgggacatg agccagggat tgggcacgtg ggcccaaggc aggcaggcgg gtatcgatga
     1981 ggagaccatc agtaggcagc ggaaggttcg tgatgtctac tatcgggagt atttgagaac
     2041 ggaagccatc gagatcgaag gcgtcgtcga catccttgcc gagctgtcga acttcgtgcg
     2101 catggcgatc gtgacgaccg cgaaacgcgt cgactttgag atcatccatg agaagcgcca
     2161 gatcagacag ttcatggatt tcgtgctcgt ccgcgaggac tacgagctag ccaagccaca
     2221 cccggagcct tatctgaccg ggttgaggcg cttcggagcc gccaaggaag agacgctggt
     2281 ggtcgaggac tcttccagag ggctgaactc ggccgtggcg gccggtatcg agtgcgtcgt
     2341 tgtggacaac gacttcacga aagcgcagga cttttcacag gccacatatc ggatcaacac
     2401 tctgagtgaa ctgaaagaca tcctcgcgtt cacctgacgg agggttaccc gttcctacgg
     2461 tcggcccggc agggtcgtcc gcgcgtgccg cagtgacggt cgattctgcc gcgatggcgg
     2521 cggaccggtt accaggtgca gtgtcagtcg ccggcgacca gccgaatctg gatcgcgcgg
     2581 aagttgaaac tgtcctggcg ggccgactcg tagctgaact caactagctg acctgcctca
     2641 agggtgcggt agccgggcat gtccatgatc tgggagaagt gtgcccacgc gtcatgcccc
     2701 gcgggtagct cctcggagct gatcgctccc cagcccttct cggcgttcca gaacttgacg
     2761 actccgcgtg ccatgtcctg accataggac ggcagctgga cacgacggat cgtgactcac
     2821 gtgcgcgtcg agcgggacgc ccggccttgc tgcgatgacg cgcctctgtc gtgccgatta
     2881 tttgggcgcg ttcaggccac gtcgaaacgc tacgagccga ctgtgccctc gttcgcgagt
     2941 gtggccgctg gcggcggcgc gccacattca gccattcgtc ggtggcggcg acgcgtcatt
     3001 ggcgagactg gccccgaggt cggccagcac ggcggcgagg catacgggta gatgcctggc
     3061 ggaagacctt cgtgaagggc ggtgagctga ggcacagggg tcgtgttgac gatcccaccc
     3121 tgccaaagtt gtgctgccca gaagtactcc cgtggccgcg ggggaccgga tcggggtggg
     3181 cgggcatcct gatcatcggg ttgtgccctc ggtaactcat gagttaccgt aaccctacgg
     3241 ttaccgatgg tcgatctggg tggtgacgtg cgtggttgcg gtgcctgcaa caggggaccc
     3301 gttcgaggcg cttggcgacc cgaatcggcg cgagatcctg cggctgctga gcagtggcga
     3361 caagccggtg caggagatcg cggccgcact gccgatcagc cggcccgccg tgtcgcgaca
     3421 cctgcgcctc ttgaaggatg ccggcctcgt cgccgaacgc cccgtcggga ctcggcgtat
     3481 ctaccacctc caagccgagg gagttcacgc cgtccaggca tatctcgaag gtatttgggg
     3541 tgacgccgcg agccggtttc gactcctcgc cgagaacact gaacggtcgc cgcagcaatg
     3601 accgatccgc tccgattttc gttcgacgtc gcgtgctcgg tcgagcacgc cttcgcggtg
     3661 tggacgtccg gtattgacgc gtggtggccg cgagatcaca cggtgaccgc cgaccccggc
     3721 ctggtcgtcg tcctgcaagg cgttgttggt ggtcgaatct ttgagcggac gccggacggt
     3781 gctgagcacg actggggcga ggtcacggcg tgggagccac cgacgctgtt ggcgtatcga
     3841 tggcatcttg gccaggacct cgccgacgcg accgatgtcg aggtccggtt tgtagcgcaa
     3901 ggtgctgccg ctacccgaat cgacgtcgag catcgcggct gggaccggct cggagctcgc
     3961 ggcgaagtgc ttcgcggtcg gaatcggctc ggctgggacg catttctctc gcactatgca
     4021 cctgcgactg cggtgccagc tactgcggaa ggagttcagt aatggcggag ggttcgaaga
     4081 gcgacccgtg ggtgctcgac acgccaccag ggacgtcgga atacacgatg tacaaggacg
     4141 aagaggccga tccgccggcg ctggtttgcc aagtcgggtc gaccacattg aagtaccagc
     4201 tgcgtgccat cgacgacctt gccgcgtggt tgcgaaagca aggtgactgg gtgccgctcg
     4261 gtgccgccga tgaacaaaag cccgcagcag aaggcactgt cgaggcatgg ggccgtgctg
     4321 acgacaatcc ggtcggcggt tggtacggcc tgcgcaaggg ctaccgcggc cgcttcggca
     4381 tgtacatgcc accactgctc gaggcactcg gtctcgccga actcacgcac gacccgcgca
     4441 acaacaggat gcgagcaacc tcatgagtac gacacgagtc atccgctaca cgacccatcc
     4501 cgagcacgcc gatgagaacg cgcggttgat tggcgacgtt ttctcagccc tggcagggaa
     4561 aatgcccgca ggtttgcact atgcggcctt gcgtctggag gacggcgtca gcttcatgca
     4621 catcgtgacg ttggacggcg aagagaaccc gctgaacgac gttccggcgt tcgctgagtt
     4681 ccagtcaaac atcatgaatc gcgttgtcgc accaccggtc acagtcttcg caacggtcgt
     4741 cggcaactac gagcttctgg tctagctgcc gtcggggaat ccgccacgat gcagcaccac
     4801 cgggaaatgc ggcagggtgg ccggcttggg cacggccgct cgccagccgt cgggataggg
     4861 ctccaatccg gcaaacagcg gtgagtcaac cgtgcctcca ccaacgagtg gttcgagcga
     4921 cttacgaatc gagcggacgg cgtcggtgcc gaatcgtgtc tgccaatccg tctcgatcgt
     4981 gctgctgagt ttgccggcgt actcctgggc ggcacgtccc ttcgctgtca ggcgaatgca
     5041 ttgtccccgg tcgggagccg gcacgggttc gatcgtcgcg cagccgaccg cctccagata
     5101 cttggccgac atcgcggccg cttccttcga aatgcccgtc agcatcggca gatcgcgcac
     5161 ccgaacaccg ccatcagtaa ggacgcgcaa cacgttcgcg ctgatggcga gtgacaccgt
     5221 cgagtcatgt tcgaagtcga tcgcgaacgc gagcagcacc
//
LOCUS       DASWVF010000628         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_196019, whole genome shotgun sequence.
ACCESSION   DASWVF010000628 DASWVF010000000
VERSION     DASWVF010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196019"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..750
                     /locus_tag="VGD55_11080"
     CDS             289..750
                     /locus_tag="VGD55_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870932.1"
                     /translation="MTNPWQISSHLRGAVLRAFGAKIGHSVLLRPRLRVKFPWKLTIG
                     DRSWIGEGVWLHNQAHLDIGADVVVSQDTFITTGTHAFRDDMALQTRPVTIEDGVWLT
                     ARCVVLAGSRIGRNALITPNTVVRGVVPPDVMFGSGEGRVLGARNVENREA"
     gene            complement(911..>3087)
                     /locus_tag="VGD55_11085"
     CDS             complement(911..>3087)
                     /locus_tag="VGD55_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0870933.1"
                     /translation="WVAHRNRRRVRLRVLAVALICAAGAAAAAAWRLAAVQRGPLPRL
                     ARAHSSVTLELVVTNDPHLSAGSSTFTQQLVVLTARAVTVTTPEGSTAISSPIVVLTT
                     AHGWLDLQPTQRVTATGRLSVPMPGALVTAVFDARGPPALVGRPSLPEVVAGHIRTGL
                     RAAATPLASGPRGLLPGLVEGDTSGLSPDLVAAFRRTGLTHIVAVSGANVAIVVGAAL
                     AIARQLGARRRAQALVGVLTIVAFVVVARPQASVLRAAAMGLVAVLALATGRRRRALP
                     ALCAAVLCLIYVDPTLSRSVGFALSVVATGALLVLAPPLRARMSTRMPGWLADALAVP
                     TAATIACAPLIASISGQVSLASIPANLLAEPAVASATILGVVTAGLAQVSMGAAQVVA
                     RVAGIPCAWLVFVARTFARLPGAAVPWPRGARGSVTLALLLVAVAALVEMRRLRVARR
                     WLGVAVRLVTVVGVIAGGFAIGNRLSRHPWPPRDWVLAACDVGQGEAIVLRSASDAAV
                     LVDTGPSPPALDQCLIALGVRSIASIVLTGGSAAAVGGLPGALHARAVGDIDGGAELA
                     TDATVRVRGWAAAAGVVAGAAPPGIVHVAGDVRWRVLAEFASARVVVLSVAGLTVLVA
                     GDLDVADEVELVSQVKSLNADVLVVPHHGASQDDRFLRAVHPRLAIVSVGRGNSQHDP
                     SADVLKTLSDVVGPAGRVVRTDEHGDSAISVNSSWPHVAPQH"
BASE COUNT          600 a         1078 c          996 g          413 t
ORIGIN      
        1 cgtttcggac ggcgattgct tgaggtcggt cacgcccatc cgtgtcggcg catgtcgcaa
       61 cccactgaag ccgtcacaac cgggtcggta ttgggcggcg cgagaactta ggctccgcga
      121 tcttccggcc gatcacgact gcgactgacg ccagtcggtg cacgaacgag ccggagggac
      181 acattgagcg agcactcgat tgatctgtcc gctgctcccg gcgagagagc cgcatgggac
      241 cgaccggccc tcatcgtgta cctgtggtcc gcgtgcgagt tgttattcgt gacgaaccca
      301 tggcagatca gttcgcacct gcgaggcgcg gtcttgcgcg cgttcggagc gaagatcgga
      361 cactccgtgt tgcttcggcc ccgattgcgc gtcaagttcc cttggaagct caccatcggc
      421 gaccgcagct ggatcggcga aggcgtttgg ttgcacaatc aagctcactt ggacatcggg
      481 gcggacgtcg tcgtgtcaca ggacacgttc atcaccacag gcacccacgc ctttcgcgac
      541 gacatggcac tccaaacgcg gccagtcact atcgaggatg gcgtgtggtt gaccgcccgt
      601 tgcgtcgtgc ttgctggcag ccgcatcggc aggaatgctc tgatcacacc aaacaccgtg
      661 gttcgcggcg ttgtgccgcc ggacgtcatg ttcgggtctg gcgagggccg cgttctgggt
      721 gcacgcaacg tggagaaccg cgaggcgtga acatcatcga gctcgccgcc tcgaccgatc
      781 cgcgctatgg cggcgcggcg acggcagtgc gggttcgcct tcgtggtctt cgggccacgc
      841 tgaggcgacg ccttccggtg ccgacgtcgt ccacatacac ggttcgacga cgttactgca
      901 tgcggcgaga tcagtgctgc ggtgctacgt gcggccagct cgagttgacc gagatcgcgc
      961 tatcaccgtg ctcgtccgtg cgaaccacac gacctgcggg accgacgacg tccgacaagg
     1021 tcttcagaac gtccgccgaa ggatcgtgct gactgttacc gcgcccgacc gacacgatcg
     1081 caagccgcgg gtgaactgcg cgcagaaacc ggtcgtcctg cgaagcgccg tgatgcggga
     1141 cgacgaggac gtcggcattc agcgacttca cctgcgagac cagttcaacc tcgtcggcta
     1201 cgtcgaggtc acccgcaacg agaacagtca gacctgctac cgaaagcacc accacccgag
     1261 cagacgcgaa ctcggcgagc acccgccatc gcacatcgcc cgcgacgtgg acgatgccgg
     1321 gaggtgcggc gccggcaaca acccccgccg ccgctgccca gcccctgacg cgcaccgtgg
     1381 cgtcagttgc caactcggcg ccgccgtcga tatctccgac cgcgcgtgca tgaagcgcgc
     1441 ctggcagccc accgacagcg gctgccgaac cacccgtgag cacgatcgag gcaatcgatc
     1501 ggacgccgag ggcaatcaga cattggtcca gcgcaggcgg cgacgggcca gtgtcgacga
     1561 gaacggccgc atccgacgct gacctaagaa cgatggcctc gccctggcca acgtcgcacg
     1621 ccgccaacac ccagtcgcgc ggtggccacg ggtgccgcga aagccgattg ccgatcgcga
     1681 agccgcccgc aatcacgccg acgacggtca ccagccgaac agccacaccc agccaccgac
     1741 gcgcgacacg caatctgcgc atctcgacga gcgcggccac cgcgacaagg agcaaggcaa
     1801 gagtgacgga accacgcgca ccgcgcggcc acggcactgc ggcgcccggc agccgcgcga
     1861 acgtacgcgc gacgaacacc agccatgcac acggaatacc ggcgacacgc gccacaacct
     1921 gcgccgcgcc catcgatacc tgcgcgagac cagccgtcac gacaccgaga atcgtcgctg
     1981 acgcgactgc gggttcggcc agcaagttcg ctggaatcga cgccaggctc acctggcccg
     2041 agatcgaagc gatgagcggc gcgcaggcga tcgtcgccgc agtcgggacg gcgagtgcat
     2101 ccgccagcca gccaggcatc cgcgtcgaca ttcgcgcgcg caacggcggc gccaacacca
     2161 agagcgcgcc ggtcgcgacg accgacaacg cgaaccccac gctgcgcgac aacgtcgggt
     2221 cgacatagat caggcacagc accgccgcac acaaggccgg caacgcccgc ctacggcggc
     2281 cggtcgcaag ggcgagcacc gcgacgagac ccatcgcggc cgcacgcagc acgctcgcct
     2341 ggggacgtgc aacgacaacg aacgccacaa tcgtgaggac gccgaccagt gcctgtgcgc
     2401 ggcgacgcgc accgagctga cgggcgatcg caagagcggc accgaccacg atggcgacat
     2461 tcgcaccgga gaccgcaacg atgtgcgtga gtccggtacg acgaaatgct gcgaccagat
     2521 caggtgacag acctgacgtg tctccctcga cgagaccggg cagcaggccg cggggtccac
     2581 tcgctagcgg tgtggcggcg gcccgcagac ccgttctgat gtgacctgcc acgacctcgg
     2641 gcagcgatgg gcgaccaacc agtgccggcg gaccgcgagc atcgaacacc gcggtgacga
     2701 gtgcacccgg catcggcaca ctgagccggc cagtcgcggt cactcgctgc gtcggttgca
     2761 ggtcgagcca tccgtgcgcc gtcgtgagaa ccacgatcgg cgagctgatc gctgtcgaac
     2821 cctctggggt cgtgacggtg accgcacgcg ccgtcaacac gaccagttgc tgggtgaacg
     2881 tcgatgaccc cgccgacaga tgcggatcgt tggtgacgac gagttccagc gtgaccgagc
     2941 tgtgtgcacg cgcgagccgc ggcaacgggc cgcgttgcac ggctgcgagc cgccacgctg
     3001 cggccgccgc tgcaccggcc gcgcagatca aagcgacggc aagaacgcgc aaccgaacac
     3061 gccgccgatt acgatgcgcg acccaag
//
LOCUS       DASWVF010000629         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_322968, whole genome shotgun sequence.
ACCESSION   DASWVF010000629 DASWVF010000000
VERSION     DASWVF010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322968"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VGD55_11090"
     CDS             complement(<1..787)
                     /locus_tag="VGD55_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY0870934.1"
                     /translation="MLDPRELYTLETSGSDAPRLDRPILVHALNGFVDAGEAVRLTHE
                     HLLNTLTHRIIGRFDVDQLHDYRARRPPMIFDADHWESYLAPELLLYAMTDAAGEQFL
                     LLTGPEPDVQWERYIAAVIGLIEHFGVRLTIGLNAIPMAVPHTRPVGITSHASRQELI
                     PDNEPWVQRVQVPASAGHLLEFRLTERGLDAIGFAAHIPHYLAQTEFPDAAERLVGAI
                     ALAGGLVLPTDELHVAGTAAKLEIDKQVADSPEAMALVGALEQQ"
     gene            1047..2672
                     /locus_tag="VGD55_11095"
     CDS             1047..2672
                     /locus_tag="VGD55_11095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY0870935.1"
                     /translation="MSVSSHSSVFADRRRVTREALERGAFDVVVIGGGIIGLSTAWHA
                     ARSGLRVAVIDAGDFAGATSSASTKLVHGGLRYLAMGDVRLVYENHAERRALGDKLAP
                     HLVQPVPFLVPLYEGGPHGRMKIGAGMLVYSALSRFDDGPGKLLSARKAAAMVPALRT
                     EGLRGCGLYYDHQMNDSRVALMVAHAAADAGAVLANYVEVVALRELDGQVAGVDVRDR
                     IDGAEFGVDARVVVNASGPWVDRIRALEDPSAAPSVRLSKGAHVIVAQREPWRAAVTL
                     PLEAGRVSFAIPWEGQLLLGTTDEPYEGEPGEVSCTDADVAQIFAEAGQAIDAAYLDP
                     AGVRYSFAGLRVLPLGDRDTQHAKRETVISRGPRGLVSVAGGKWTTFRRIGMEVLEHV
                     HELLPGTEIDRADAVLPGAAHPDSVARVLRASTPDLPDDVVAHLAMHYGTYAHELASL
                     MALDPTLARRIHPEGPDVWAQVVYAGSHEWACNADDVLRRRTTVEVRGLATPAVRAEV
                     TAMLTGTRAGFDVPRPRLGRIALWPRRRQAAGA"
     gene            2759..>3428
                     /locus_tag="VGD55_11100"
     CDS             2759..>3428
                     /locus_tag="VGD55_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0870936.1"
                     /translation="MPVPTDNPHINFFQAITIGVMQGVTELFPVSSLGHSVLVPALIG
                     GSWETLVSQEHASESPYLAFIVGLHVATALALLLFFWRDWVRLIRGFAASLPRLARRL
                     FRQPVDGPLLRTVHEKMAWLIIVATIPAGVIGLALEHVFRTLFAKPLAAAIFLTINAG
                     ILFAGERLRRRSKERVDATLAAEADAKATAHDDPTYDPEHDPENDVAITRRFTFRDAA
                     VVGVF"
BASE COUNT          517 a         1106 c         1201 g          604 t
ORIGIN      
        1 actgctgctc tagggcaccg accaacgcca tcgcttcggg agagtcggcg acctgcttgt
       61 cgatttcaag cttggcagct gtgccggcga cgtgcagctc gtcggttggc agcacgaggc
      121 cgccggccag cgcgatggca ccgaccaatc gctctgcggc gtcggggaat tcggtctgcg
      181 cgaggtagtg cgggatgtgc gcggcgaagc cgatcgcgtc gagcccgcgt tcggtcagcc
      241 tgaactcgag cagatgcccg gcgctggccg gcacctgcac gcgctgcacc cacggctcgt
      301 tgtcggggat cagctcttgg cgtgatgcgt gcgaggtgat gccaacaggc cgggtatgtg
      361 gcacggccat ggggatcgcg ttcaagccaa tcgttaggcg gacgccgaaa tgttcgatca
      421 acccgatgac ggccgcgatg tagcgctccc actggacatc gggttcaggg ccggtcagga
      481 gcaggaattg ctcgccggcg gcgtccgtca tggcatacag cagcaactca ggggctaggt
      541 aactctccca gtggtctgcg tcaaagatca tcggtggccg acgcgcccgg tagtcgtgga
      601 gctggtcgac gtcgaaccgg ccaatgatcc gatgcgtcaa cgtgttgagc aggtgctcgt
      661 gggtgagccg caccgcctcg cccgcgtcga cgaacccgtt caacgcgtgg acaagaatcg
      721 ggcggtcgag cctgggcgcg tcggatccag aggtttcgag ggtatagagc tcgcgtgggt
      781 cgagcacggt tgcgggcctc cctctatccg gccttctagt cggccgccga tctgccgaag
      841 tgacgtgagt acccattctc tcgcaccgcg aggcgttcgg tcggacggcg gtcttggcgc
      901 gttcagggga tgttcaaccg cgagatccga cttggacacg acattgcggc cgcgaaagcg
      961 ggagcattgg acgagcaagg gcccgcgcag gataggaaga gcgtgagcag acggtaacga
     1021 cgagtggcga agggagggcg tcgtaggtgt cagtgtcctc gcacagttct gtgtttgccg
     1081 atcggcggcg ggttacccgc gaagccctcg aacgcggcgc ctttgacgtg gtcgtcatcg
     1141 gcggcggcat catcggcttg tcgaccgcct ggcatgccgc gcggtcgggc cttcgggtcg
     1201 cggtgatcga cgccggcgat tttgcgggcg cgacctcaag tgcttccacc aagctggtac
     1261 acggcggctt gcgctacctg gccatgggcg acgtccggct ggtgtatgag aaccacgcgg
     1321 agaggcgcgc gctcggcgac aagctcgcac cgcaccttgt gcagccagtg ccgtttctgg
     1381 tgccgctcta tgagggcggg ccgcacgggc ggatgaagat cggcgccggc atgctcgtct
     1441 actcggcgct ctcgaggttc gacgacggtc cgggcaagct gctgtcggcg cgcaaggcgg
     1501 ccgcgatggt gccagctctg cgcaccgaag ggttgcgcgg ctgtgggctg tactacgacc
     1561 atcagatgaa cgacagccgg gtggcgctga tggttgcgca cgctgcggcc gatgcgggcg
     1621 cggtcctggc gaactacgtc gaagtggtcg cgcttcgcga gctcgacggc caggtcgcgg
     1681 gcgtcgacgt acgcgaccgc atcgacggtg ccgagttcgg cgtcgatgcg cgcgtcgttg
     1741 tgaacgcaag cgggccatgg gtcgaccgca ttcgcgcact cgaagacccc tcggctgctc
     1801 cgagtgtgcg gctgtcgaaa ggcgcacacg tgatcgtcgc gcaacgagag ccgtggcgag
     1861 ccgccgtcac gttgcccctt gaagccggcc gggtctcgtt cgcgattccg tgggaggggc
     1921 aattgctgct cggcactacc gacgagccgt acgagggaga gcctggcgag gtctcgtgta
     1981 ccgacgccga cgtggctcag atctttgccg aagcgggtca ggcgatcgat gcggcttatc
     2041 tcgacccggc tggggttcgc tactcgttcg ccgggttgcg cgtgctcccg ctgggcgacc
     2101 gcgataccca gcacgccaag cgcgagacgg tgatcagcag ggggccgcgt ggcctggtca
     2161 gcgtggccgg tggcaagtgg accacgttcc ggcggatcgg catggaagta ctcgagcacg
     2221 tgcacgaact cctgcccggt accgagatcg accgcgccga cgccgttttg ccgggcgccg
     2281 cgcatcctga ctcggtggcg cgggtgctgc gggcttcgac gcctgacctg cccgatgacg
     2341 tcgtcgccca cctggccatg cactacggca cctacgccca cgagcttgcg tcattgatgg
     2401 cgctcgaccc aacgcttgcg cgtcggatcc atcccgaggg tcccgacgtg tgggcacagg
     2461 tcgtctacgc gggcagccac gagtgggcct gcaacgccga cgacgtcctt cggcggcgga
     2521 ccacggttga ggtgcgcgga ctcgccacac cggcggttcg ggccgaggtc accgcgatgc
     2581 tgacaggtac gcgggccggc ttcgacgtgc cccggccgcg gctcggccgg attgctctgt
     2641 ggccgcgtcg gcgccaggcg gccggagcct aaccggtcgt ttgggtcatg aacgggctcg
     2701 tcggcccgta tcgtttggcg gaagcatccg ccgtccgctg acccatcgag gctcccgcgt
     2761 gccagtcccg actgacaatc cgcacatcaa cttcttccag gcgatcacga tcggggtcat
     2821 gcaaggcgtg acggagcttt tccccgtctc cagccttggc cactcggtac tcgtgcctgc
     2881 cctgatcggt ggctcgtggg agaccctcgt cagccaggag cacgcatcgg agtcgcccta
     2941 cctggcgttc atcgtcgggc tgcacgtcgc tacggcgctt gcgctcctgc tgttcttttg
     3001 gcgcgactgg gtgcggctta tccgtgggtt cgccgcgtcg ctgccgcgcc tggctcgccg
     3061 cctgttccgc cagccggtcg acggaccttt gctgcggacc gtgcacgaga agatggcctg
     3121 gctgatcatt gtcgcgacga tccccgccgg agtgatcggg ctcgccctag agcacgtgtt
     3181 tcgcacgcta ttcgcgaagc cgcttgccgc cgcgatcttc ctgacgatca acgcgggcat
     3241 cttgttcgcc ggcgaaagat tgcgccgccg gtcgaaggag cgcgtcgacg caacgttggc
     3301 cgctgaggcc gacgctaaag ctacggcgca cgacgacccc acctacgacc ccgagcacga
     3361 cccggagaac gacgtcgcga tcacccgccg gttcacgttc cgcgacgccg ccgtcgtcgg
     3421 ggtgttcc
//
LOCUS       DASWVF010000630         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_145089, whole genome shotgun sequence.
ACCESSION   DASWVF010000630 DASWVF010000000
VERSION     DASWVF010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145089"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..799
                     /locus_tag="VGD55_11105"
     CDS             53..799
                     /locus_tag="VGD55_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870937.1"
                     /translation="MTSARPLQAEDVTRAAWIGPRLAAFASGVNGVIPNGFEAYARIL
                     HPALSDGDQLTRWSEVAATTGTVDHALMQFHSLVGVSPFDSQVTSGAWTGSTPEQGNL
                     EPESLAALLAVLAQHTPAGEQCWFCLWEGYGMPFGTERSTGPGAPRIDFEARVELPQR
                     NYVLFGGPLESAPEIGHWATPDWFLPHSPNIFWPDDRSWCVASEIDLYCTYVGGSRAV
                     IDELLVDERLEVWEANLDDPVVHDSDELNG"
     gene            complement(796..1587)
                     /locus_tag="VGD55_11110"
     CDS             complement(796..1587)
                     /locus_tag="VGD55_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY0870938.1"
                     /translation="MSEPNTTPSAEVLLAALRNSRARLVETVTPLTPEQLRSQSYDTE
                     WSIAQVLSHMGTGAIFFKLILEAGLAGEPAPGIDVMTPIWDLWNAKSPEEQAHDGLES
                     DAALLGAFEALDAQQRADWEMDLWGTHRDFAGVVRMRVSEHAFHTWDVVVALDPKAQL
                     GPDSVDVIIDTLAPLVARVSKPLDEQLVIGVETEDPDRNFVLTTGADGATLEPLDARS
                     DADRLLLLPAEAFARLVFGRLDADHTPPGADDPILEKLRPVFPGI"
     gene            complement(1671..>2080)
                     /locus_tag="VGD55_11115"
     CDS             complement(1671..>2080)
                     /locus_tag="VGD55_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870939.1"
                     /translation="QRTTFTPTGTEDDIVGMGYENTTDPSFAPYDHPDQKAVAEVMTG
                     VDLGLPGPQWSRTYPADPARAMTATVDQALFDAVTAVSPGPVDPVANPNARRVGALQV
                     DYGWLAFPGAYWYAEQEPVHSFYSRSQDFTKSF"
BASE COUNT          337 a          674 c          714 g          355 t
ORIGIN      
        1 ccgtgcacga acagccgacg ctcgacgagg ccgcgctcga cgaggcggcg ctgtgacgtc
       61 ggcgcggccg ttgcaggccg aggacgtcac gcgcgcagcg tggatcgggc cgcggctcgc
      121 ggctttcgcg tccggcgtga acggcgtcat tccgaacggg ttcgaggcgt acgcgcgcat
      181 tctgcacccg gctttgtctg acggtgatca actcacgcga tggagtgagg tcgccgccac
      241 gaccggtacg gtcgaccacg cgctcatgca atttcactcc ctcgtcggtg tttcgccctt
      301 tgattcccag gtgacctcag gtgcctggac cggctcgacc cccgagcaag gcaaccttga
      361 gcccgaatcc ttggcggcac tgctcgccgt cctggcacag cacaccccgg caggcgagca
      421 gtgctggttc tgcctgtggg agggctatgg catgccgttc ggcacggagc gctcgaccgg
      481 tcccggcgca cctcggatcg acttcgaggc gcgggtcgaa ttgccgcaac gcaactacgt
      541 gctgttcggc gggccgcttg aatcggcgcc ggagatcggt cactgggcga cgccggactg
      601 gttcctgccg cactcgccga acatcttctg gcccgacgac aggtcgtggt gcgtcgccag
      661 cgagatcgac ttgtattgca cttacgtcgg cggatctcgc gcggtgatcg acgagctgct
      721 cgtggacgaa cggctcgagg tgtgggaggc caacctcgac gacccggtcg tccacgacag
      781 cgacgaacta aatggctaga tcccggggaa gaccggccgc agcttctcga ggatcggatc
      841 gtcggcgccg ggaggcgtgt gatcggcatc gagtcggccg aagacgaggc gcgcaaaggc
      901 ttccgcaggt agcagcagca ggcggtcggc gtcggatcgc gcgtcgagcg gctccagcgt
      961 tgcgccgtca gcgccggtcg tcagcacgaa gttgcggtcc ggatcttcag tctcgacacc
     1021 gatgacgagc tgttcgtcga gcggcttgct cacgcgcgct acgagaggcg cgagcgtgtc
     1081 gataatcacg tcgaccgagt cggggccgag ctgcgccttc gggtcaagcg ccaccacgac
     1141 gtcccaggtg tggaacgcat gttcactcac ccgcatccgc acgacgccgg cgaagtcgcg
     1201 atgcgtaccc cacaagtcca tctcccagtc ggcccgctgt tgcgcgtcga gcgcctcgaa
     1261 cgcgccgagc aatgcggcgt ccgactcaag tccgtcgtgc gcctgttctt ctgggctctt
     1321 cgcattccag agatcccaga ttggagtcat cacgtcgata ccgggcgccg gctcgccagc
     1381 gaggccggcc tccaggatga gcttgaagaa gatcgccccc gtgcccatgt gcgacagaac
     1441 ctgtgcgatc gaccactcgg tgtcgtacga ctggctccgc aactgctcag gcgtcagcgg
     1501 ggtgacggtc tcgaccagcc gcgcgcgtga gttgcgcaac gctgcaagca agacttcggc
     1561 ggacggcgtg gtgttcggtt ccgacatagt gacccgctca acagttgaag gcacgaccag
     1621 atgcctccac gttagtcgag cggcgcgccc agtgcggtgg gcgggcgggt tcagaaggac
     1681 ttcgtgaagt cctgcgaccg cgagtagaag gagtgcaccg gctcctgctc ggcgtaccaa
     1741 taggcgccag ggaacgcgag ccagccgtaa tcgacctgca gtgccccaac gcgtcgagca
     1801 ttcgggttgg cgaccgggtc gactggccca ggggatacgg ccgtgacggc gtcgaacagc
     1861 gcttggtcca cggtggcggt catcgcccgc gccggatcgg ctgggtaggt gcgcgaccat
     1921 tgcggcccgg gcaggccgag gtcgacgccc gtcatcacct cagccaccgc cttctggtcg
     1981 ggatggtcgt acggcgcgaa tgatgggtcg gtggtgttct cgtaacccat gccgacgatg
     2041 tcgtcctcgg tgccggtggg cgtgaacgtg gtgcgctgcg
//
LOCUS       DASWVF010000631         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_502015, whole genome shotgun sequence.
ACCESSION   DASWVF010000631 DASWVF010000000
VERSION     DASWVF010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502015"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGD55_11120"
     CDS             <1..832
                     /locus_tag="VGD55_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0870940.1"
                     /translation="AAHLAYASAAHYRLVSTGSGPPLLQLHGLGGTCDQLARYSMDLV
                     VGSLLAPDLRAHGQTALVGAPDAFHFNGLADDLVALLDVLHYPRAAVVGVSMGAGVAV
                     NLALRYPERVSALLLIRPAWTDEPSPVHLSALSHAGDLLQRMGNVVGARVFADSAEYR
                     HVLGISAAAAASLLDQFRDPLAAQRAVRLVQMPLSNPFKRLADLGALDVPTTVVAAPA
                     DPLHPVEVADAWAAHIPGSAWFQTASRDSDPAAYHRDLGTSVERFVASLVPAGGLQRG
                     "
     gene            825..>1598
                     /locus_tag="VGD55_11125"
     CDS             825..>1598
                     /locus_tag="VGD55_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY0870941.1"
                     /translation="MADIGDGYGVTTTEVPTDVPSSMHPSTMHPSSMHPSSMHSAFSE
                     ERRAKILEVVTSRGRIRTSELAELLGVAEPTVRRDVADLDRQRLVRRTHGGVLAMRPA
                     YEPELAARAQLNVPGKRAIAQACLALIDDRDSVFLDSGTSNEMIAEALRRALTEAPPG
                     SVVKPAHVTVLTNAIKIASLLGDVPTVRHTVLGGQFRSLGGCMVGPLALENLQQFTVN
                     TAFIGVTGLSDNGFSVSDLGDAQLKEAIMDRARRVIVPMD"
BASE COUNT          243 a          520 c          528 g          307 t
ORIGIN      
        1 ggccgcgcac ctcgcttatg cgagcgctgc gcactaccgg ctggtcagca cggggagcgg
       61 tccgccgctt ctgcagctac acggtcttgg cggaacctgc gaccagctcg ctcggtactc
      121 gatggatctc gtggtcggct cgctgttggc tcccgatctg cgcgcccacg gccagacggc
      181 acttgtcggc gcgccagacg cgtttcactt caacggcctc gccgatgacc tagttgcgtt
      241 gctcgacgtg ctgcactatc cgcgtgctgc cgtcgtcggt gtctcgatgg gcgcgggagt
      301 cgcggtcaac cttgcgcttc ggtatccaga acgggtcagc gccctgctac ttatccggcc
      361 ggcatggaca gatgagccga gtccagttca tctctccgcg ttgagccacg ccggtgatct
      421 cctgcagcgc atgggcaatg tggtcggtgc gcgcgtcttc gccgattcgg ccgagtacag
      481 gcatgtcctc ggcatatcgg cggcggcagc ggccagcttg cttgaccaat tccgtgatcc
      541 gttggctgcg cagcgcgcgg ttcggcttgt gcagatgccg ctgtcgaatc cgttcaagcg
      601 gcttgccgac ctcggcgcac tcgacgtccc cactaccgtc gtcgcggccc cagccgatcc
      661 actgcatccg gtcgaggtcg ccgacgcatg ggctgcccac attcccggtt ccgcgtggtt
      721 ccagaccgcg tcgcgtgaca gtgatccggc cgcgtaccac cgcgacctcg gcacatcggt
      781 cgaacggttt gtcgcgtcgc tcgtacccgc aggaggactg caacgtggct gacattggcg
      841 atggatacgg cgtgacaacg accgaagtac ccacggatgt gccgtcgtcc atgcacccgt
      901 cgacaatgca cccgtcgtcg atgcacccgt cgtctatgca cagcgcgttc agcgaagagc
      961 ggcgcgcgaa gatcctggaa gttgtcacgt cgcgcggccg gatccgaacg tcggaacttg
     1021 ccgaactgct cggcgttgcc gaaccgaccg tgcggcgcga cgtggccgat ctcgatcgcc
     1081 aacggttggt gcgacgcacc cacggcggcg ttctggccat gcgcccggca tacgagccgg
     1141 agctggctgc ccgtgctcag ctgaacgtgc cagggaagcg ggcgatcgcg caggcgtgcc
     1201 tcgcgctgat cgatgaccgc gactcggttt tcttggacag cggtaccagc aacgaaatga
     1261 ttgcggaggc gttgcggcgg gccctgaccg aggcgccgcc ggggagtgtt gtgaagccgg
     1321 cgcacgtgac cgtgctgacg aacgcgatca agattgcgtc gctgcttggt gacgtgccga
     1381 cggttcggca caccgttctc ggcgggcagt ttcggtcact tggtggctgc atggtcggtc
     1441 cgcttgcgct ggagaacctt cagcagttca cggtcaacac cgcattcatc ggtgtcacgg
     1501 gcttgtccga caacgggttc agcgtctccg acctgggtga tgcgcagttg aaggaagcga
     1561 tcatggaccg cgctcgccgg gtgatcgtgc cgatggat
//
LOCUS       DASWVF010000632         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_502033, whole genome shotgun sequence.
ACCESSION   DASWVF010000632 DASWVF010000000
VERSION     DASWVF010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502033"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGD55_11130"
     CDS             <1..541
                     /locus_tag="VGD55_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0870942.1"
                     /translation="IAPHLFHRDGVNAIPYQLELALPHMAALTAEGIRADLAASREHL
                     AAQGFAMSATGIVGFCMGGSIALIAASEQPYGAAVTFYGRGIIEGRFGFRPLGELAPK
                     LLSPWLGLYGDLDPSIPVGEVEALRVAAAAAQVPTMVVRYAEAGHGFHCDARPDVYNE
                     AAADDAWTKTLDWFARYLA"
     gene            complement(615..1346)
                     /locus_tag="VGD55_11135"
     CDS             complement(615..1346)
                     /locus_tag="VGD55_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0870943.1"
                     /translation="MATNTIPGRHRVLVVDDEPSISDLVATALRFEGFEVSTASTGNE
                     ALEAVEASRPDLVVLDVMLPDFDGFEVARRLAAKGKSVPVLFLTARDATDDKVLGLNV
                     GGDDYVTKPFSLAELVARVHAVLRRSHGNGHGTRISFSDLEMDDDTREVFRGGRLIEL
                     TATEYRLLHYLLVNARKVLSREQLLDHVWDYDFGGDSGVLETYISYLRKKVDVDAPPL
                     IHTVRGVGYTIRLSNRPADAVARPS"
     gene            complement(1456..>2391)
                     /locus_tag="VGD55_11140"
     CDS             complement(1456..>2391)
                     /locus_tag="VGD55_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0870944.1"
                     /translation="RVEQARAVYEGVAIYPNVDALWGVADDIDLIVVATPNRDHAPIA
                     HAAIAAGIAVVIDKPVAVTVGEAESVIEAAAARGVPLSVFQNRRWDGDFLTLQRLLGE
                     GALGDVNRFESRFERWRPVPKGGWRESGSVSDAGGLLFDLGPHLIDQALQLFGPVADV
                     YAEVDRRRQSVETDDDVFIALHHASGVRSHLWCSAVASDLGPRLRVLGTRAAYVKYGL
                     DVQEDALRLGGRPDRDAAWGTETSDRWGTLGVPGETTPVATEPGAYQRYYEGVAAALR
                     GDGAMPVDPHDSVAGLRIIEAARRSAQDREVVSLP"
BASE COUNT          420 a          789 c          790 g          392 t
ORIGIN      
        1 gatcgcgccg cacctgttcc atcgggacgg ggtcaacgcg atcccgtacc agctggagct
       61 cgcactccct cacatggcgg cgttgactgc tgagggaata cgcgccgacc tcgcagcttc
      121 acgcgaacac cttgcggcac agggattcgc gatgtcggcg acgggcatcg tcgggttctg
      181 catgggtggg tcgatcgcgc tgattgcggc gtcggagcag ccgtacggcg cggccgtcac
      241 gttctacggc cgcggcatca tcgagggacg gttcggcttt cgaccactcg gcgaactcgc
      301 gccgaagctg ctgtcgccgt ggctcgggtt gtacggcgat ctcgacccga gcatcccggt
      361 cggcgaggtc gaggctttgc gcgttgccgc agcggctgcg caagtgccga cgatggtggt
      421 ccgctacgcg gaagcgggtc acgggtttca ttgcgacgcg cgaccagatg tctacaacga
      481 ggccgccgcc gacgacgcct ggaccaaaac cctcgactgg ttcgcccgct acctcgcgta
      541 ggcgagatcg tggagtgccc agcgtgctct aggtcgcttc agactccacg atgatgtggt
      601 ggccggcggg cgggttagct cgggcgggct actgcgtcgg ctggtcggtt ggacaaccgg
      661 atcgtgtacc cgacgccacg gacagtgtga atgagcggcg gcgcatcgac gtccaccttc
      721 ttgcgcaagt agctgatgta ggtctcgagc acgccagaat cgccgccgaa gtcgtagtcc
      781 cacacgtgat cgagaagctg ctcccggctg aggaccttgc gcgcattgac gagtaggtag
      841 tgcagcaggc gatactcggt agcggtgagt tcgatcagcc ggccgccgcg aaagacctcg
      901 cgggtgtcgt cgtccatctc gagatcgcta aacgagatgc gggtgccgtg accgttgccg
      961 tggctgcgac gcaagacggc gtgcacgcga gcgaccaact ccgccagtga gaacggctta
     1021 gtcacgtagt cgtcgccacc gacgttcagg ccgagcacct tgtcgtcggt cgcatcacgc
     1081 gcggtcagga agaggaccgg aacgctcttg cccttggcgg ccagccggcg agcgacctcg
     1141 aatccgtcga agtcgggcag catcacgtcg agcacgacca ggtcgggccg cgatgcctcg
     1201 accgcctcga gagcctcatt gccggtgctg gcggtgctga cctcgaatcc ttcgaaccgc
     1261 agtgcggtcg cgaccagatc ggaaatgctc ggctcgtcat cgacgaccag cacccgatgc
     1321 cggccgggga ttgtgttcgt agccacaagc gccaccctcc tgagggcccc cactctgccg
     1381 ccgaaggctg cacaaaggct gggagaaacc tggaggattc ctgggaattc cccaagacct
     1441 ccggccgggc ccacatcagg gcagggagac gacctctcga tcctgcgccg agcggcgggc
     1501 ggcttctatg atccgcaagc cggcaaccga gtcgtgcggg tcgaccggca tcgcgccgtc
     1561 gccgcgcaag gctgcggcaa ctccctcgta gtagcgctgg tacgcaccgg gttcggtcgc
     1621 gacaggcgtc gtctcccccg ggacgccgag ggtgccccag cgatcggagg tctcggtccc
     1681 ccatgcggca tcgcggtcag gccggccacc gagtcgcagt gcgtcctcct gaacgtcgag
     1741 cccgtatttg acgtaggcgg cccgagtgcc gagcacccgc agccgcggtc cgaggtctga
     1801 cgcaaccgcg ctgcaccaca agtgactgcg cacaccgctg gcatggtgca aggcgatgaa
     1861 gacatcgtca tcggtctcga cactttggcg acgccggtcg acctcggcgt acacatcggc
     1921 caccggtcca aagagttgca gggcctggtc gatgaggtgt gggccgagat cgaacagcag
     1981 ccccccggcg tccgatactg aaccgctttc acgccagccg cccttcggaa caggccgcca
     2041 ccgctcgaac ctcgactcga accggttgac gtcgccgagc gcgccttcac ccagcaatcg
     2101 ttgcagggta aggaaatcgc cgtcccatcg tcggttttga aaaacactga gagggacgcc
     2161 gcgggcagcc gccgcctcga tgacggactc tgcttcgccg acagtgacag caaccggttt
     2221 gtcgatcacg accgcgatgc cggccgcgat cgccgcatgg gcaatcggag cgtggtcgcg
     2281 gttcggagtc gcaacgacga tcagatcgat gtcgtcggca acaccccaca gagcgtcgac
     2341 attcggatag atcgcgacgc cttcgtagac ggcgcgagcc tgctcgacac g
//
LOCUS       DASWVF010000633         9463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_297796, whole genome shotgun sequence.
ACCESSION   DASWVF010000633 DASWVF010000000
VERSION     DASWVF010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297796"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..752
                     /locus_tag="VGD55_11145"
     CDS             354..752
                     /locus_tag="VGD55_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0870945.1"
                     /translation="MTTTVAPRGALGIAEVLAAARARLGRVTAEQAHAEVRAGAVVVD
                     IRPAAQRLAEGEIPGAVVIERNVLEWRLDPRSDHRLPMASFGLRVIVVCSEGYTSSLA
                     AVSLLDLGIAGATDLEGGFKAWQAAGLPVK"
     gene            850..2217
                     /locus_tag="VGD55_11150"
     CDS             850..2217
                     /locus_tag="VGD55_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0870946.1"
                     /translation="MEQVDVVVLGMGPGGEYLAGRLAETQLDVIGIDQRLVGGECPYF
                     GCVPSKMMIRAANALAEGRRIPLLAGDSRVTADWAPVAARIRNEATDDWNDKVAVDRF
                     EGKGGHFVRGHGRLTSPNTVSVGDREFEARRGIVLNTGSDPAVPPIPGLAGTPFWTNR
                     EAIQTTHVPASLIVLGGGAIGVELAQVFARFGSRVTVVEAAPRLVYFEEPDASAAVAE
                     VFGREGISVRVGTSATGVQHDGTGFTVDLAGGDSVHAEQLLVATGRRVDLAAVGLDTV
                     GVDVTARAVPVDSRMRVAPGVWAIGDMTGKGAFTHVSMYQAEIAVADILGISGIADAE
                     YAALPRVTFTDPEVGSVGMTQEQATAAGIDVLVGRAPVSSSARGWIHGPGNDGFIMLV
                     GDANRGVLVGATVVAPCGGEVMSALVVAIHAEVPLERLLSMIYAYPTFHRGILDALKD
                     LRRPA"
     gene            complement(2223..2786)
                     /gene="lepB"
                     /locus_tag="VGD55_11155"
     CDS             complement(2223..2786)
                     /gene="lepB"
                     /locus_tag="VGD55_11155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0870947.1"
                     /translation="MDTPKKVHWGREWVILIVLTVIGTFLLRTYAVQSYRIPSGSMEK
                     TLHGCTPGCNDDRVLVDKLSYKLHGIHRHDVVVFQATGNWIPVVGGREDVIKRVIGLP
                     GDTVQCCDAQGRVELNGVSQNEPYVFEDNHYAFGPVVVPKGQLFVMGDHRSDSSDSRY
                     NGTIPESSVVGHAFVRIWPITRIHTLH"
     gene            complement(2845..4020)
                     /gene="manA"
                     /locus_tag="VGD55_11160"
     CDS             complement(2845..4020)
                     /gene="manA"
                     /locus_tag="VGD55_11160"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEY0870948.1"
                     /translation="MVNPVMPYAWGSREAIATLQGRPVGDVPEAELWMGAHPQATSLL
                     ERDDGSPGEALDALIAADPLGVLGERCVERFGPRLPFLLKVLAADQPLSLQVHPNAEQ
                     AQRGFAAEEGAGIDPAAPNRCYRDPYPKPELLVALTDFHVLQGFRAASDAAAALKALD
                     VFGLDPLVEALQGGTPTGEVFLRLIEWPADDRLRLVSDVRLASAASATGALDGIAHWI
                     NWLAVRYPNDPGVVGVALLNYLVLRPLEGLYVRPGQIHAYLHGTGVEILGGSDNVIRG
                     GLTPKHVSAADLRAILDVGAVEPALVPATAATDGGDQTWPTPQPELELRRVQIVTAKR
                     LAPAGPSVLLCTEGSVEVGDGDGTVSLRPGESAFAFAGTDLDVTGRGVLMRANPGPC"
     gene            4202..4978
                     /locus_tag="VGD55_11165"
     CDS             4202..4978
                     /locus_tag="VGD55_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0870949.1"
                     /translation="MSFTELLRGSRPQQLAPATPGTLDKIATAVREIGIVVGLFVLWR
                     LASAISIGGDSGAFARGRWIWRLERTLRLPSELTVQNGLLRHHDLVQVVNLFYLAAHV
                     GGLVLFLPWLFIRHREHYRHWRNVIAVFTGLSVLIQLLSVAPPRLLPQYGFVDTGALY
                     HESAYQHLGQGLIGQLSSMPSIHVGWAIAIAMAVISTSTSRWRWLVVAHPILTVYAVV
                     ASANHFWLDCVAAAGLLLLVIAGVNALARHQAAKRKPAVT"
     gene            complement(4971..5231)
                     /locus_tag="VGD55_11170"
     CDS             complement(4971..5231)
                     /locus_tag="VGD55_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEY0870950.1"
                     /translation="MCTYMTATLAAQGSAKGQGDWFNVTDASVYLDHPVHAQAGHTLN
                     IDFRNPSLGPAARVAAELTADSARALAHAILEILDAAPPGLT"
     gene            complement(5234..5977)
                     /locus_tag="VGD55_11175"
     CDS             complement(5234..5977)
                     /locus_tag="VGD55_11175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0870951.1"
                     /translation="MYDGIFAETTTVTGDGGDLIEAYFARPLGPDPVGSVVVIHHLPG
                     YDAATKEIARHFAALGFAALMPNLYSREAPGARPDDAAAMVRAMGGVPDARLVGDVAG
                     AAAHLRALPYTNGKVGVIGYCSGGRQSFLAATSTPLDAAVDCYGAFVVTAPPEASGLK
                     WLPIVKRAPLLGCPLLGLFGAEDKNPSPEEVGELKKALDDAGKTYEFHSYDDAGHAFF
                     ATDRPSYRPAAAVEGWQRITEFFTRYLGA"
     gene            6114..8027
                     /locus_tag="VGD55_11180"
     CDS             6114..8027
                     /locus_tag="VGD55_11180"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEY0870952.1"
                     /translation="MLRFRRLGVGVAIGGTVAGLGLMGLVAAAAQASASTTIDIRGSW
                     SEVTTTQGATYPQTVTFVTENFSTGAVTGTDVGNGQSLTVTGTIKGSTLTTHSVEAGY
                     SSDAVGTITKSGATLAWNGTFTDSVGHPNGTFTATLTTPASGTTSTSGAGILDVRGLW
                     DGYYIQPGGAKYHNPVVFVTENFSTGQLTGTDNGTDGAATITGTISGRTINATIAQPP
                     SYSANVTWVISADGTKLVSGGITDPTAPNAFVLFRHGAPVGAGSAVTSGAGVTSGPAP
                     ITALPTPSQAFSSPGRIIASAAISIAALLFITFPANLFNKTFEENYDVIAAWWGPRLR
                     RFRRKPSTEGPDAEGPADESAPDTAAKKDRLKFGVVVIAGAILGGLLNPKFGANSASV
                     NSFIATLLSVITGTVVGTAVGFLYRKRKKHDLTRHFEALPAGLAIAALCVVLSRVSDF
                     QPGYLYGVVCGVAFAGSLAKNENGHLVALGHLATIAVAVIAWFLWVPVNHAAIHHTGV
                     FPLVILDDLLASLFVGGLVGSVINLIPVRFMPGHALASWHRGAWMAVFGVAAFGMTEI
                     VVFPSQHNHAGHAPLATILVLFVLFGGGSLAFREYFSRRDKRLELAAAPADGPPPGVP
                     TASASPAPETSGL"
     gene            8072..>9463
                     /locus_tag="VGD55_11185"
     CDS             8072..>9463
                     /locus_tag="VGD55_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870953.1"
                     /translation="MDATADTLQKALRINLDPRWYGTIAEIGAGQEVARWFFRAGGAA
                     GTVAKSISAYDMAVSDAVYGKSGRYVSMPRLQAMLDLEFRLNIERLDAERGDSNAFFA
                     FADTVTARSYSGGNECHGWMGVKFQAQPHDEPSQIIMHVRMLDVEAALQQEALGIVGV
                     NLLHAAFFQHHEPETLVESLLDRLTTGRIEIDMIELKGIEFRAVDNRLMALKLVQVGL
                     SGAAMFGPDREVLQPSEVLRKKAILVERGSFRPPTVVNIDMLESALAKFESDPEVSSR
                     EILMLTELTMANLLAGGGDVDRRDFLARADLLAACGMTVLISDYVEYHRLAAYLAWRT
                     DGRIGMVMGVPSLIDLFNESSHAKLPGGILESFGRLFKNDLKLFVYPMLRDDRVTTVD
                     DLVVGGDLQPLYDYLARRGSFVGLDNYKPDYLPILSRDVLRRIASGDAMWESMVPPEV
                     GELIKKRGFFGYHR"
BASE COUNT         1612 a         3148 c         3132 g         1571 t
ORIGIN      
        1 cgtagtcggc cgcgcgacga ccgtggcgtt tggacggtct cggcgggacg gaagggaaga
       61 aagaccaggg cagtgaggga cggcgcggcg tgcgcgaggc tggtaggtcg cctcttagcg
      121 ggtggcgccg tgacgtgcgc agctgcaaca cagcgcgctg gcgtgccgga caaggtccag
      181 atgcacgcgg gcgtggagat gcgctccacc gctcagggca ttgaaggcaa tagccatgct
      241 gacaacggta gcaacgggtg ctcgcttatg tctactattc cgatagccga agattagata
      301 gactcacccg gctccaacag tcaaggacca cgacgagtga gagaacacag gtcatgacaa
      361 cgacagttgc gccccgcggt gcgcttggga tcgcggaggt cctcgcagca gcacgggcgc
      421 gccttggtcg ggtgacggct gaacaggctc acgccgaggt gcgcgccggc gccgtagtcg
      481 tcgacatcag gccagccgct caacgcctcg ccgaaggcga gatcccgggc gcggtcgtga
      541 tcgagcgcaa cgtgctcgag tggcgactcg acccacgcag cgatcaccgg ctgccgatgg
      601 caagctttgg gctgcgcgtg atcgtcgtct gctccgaggg ctacacctcg agccttgccg
      661 cggtgagcct gctggatctc ggcatcgcag gcgcgaccga tctcgagggc ggcttcaagg
      721 cctggcaggc cgcaggcctc cctgtgaagt gaaggcagca ggacttccgg tcgagtaacc
      781 gcactaccgg catgtacaag acgggcgtca caatcaaggg gtccgatcgc cgtccggccg
      841 gaggctctca tggaacaagt cgacgtcgtc gttttgggca tgggcccagg tggggagtac
      901 ctcgccggcc ggctggccga aacgcagctc gatgtgatcg gaatcgacca acgcctggtg
      961 ggcggtgagt gtccttactt tggctgcgtg ccgagcaaga tgatgatccg cgcggccaac
     1021 gcacttgcag aaggccgtcg aattcccctg ctggcaggcg attctcgggt caccgccgat
     1081 tgggcaccgg tggcggcgcg cattaggaat gaagccactg atgactggaa cgacaaggtg
     1141 gcggtcgatc gcttcgaggg caagggcggt cacttcgtcc gaggtcatgg ccgcctgacg
     1201 agccctaaca cggtcagcgt cggcgatcgc gaattcgagg cgcgccgggg gatcgtcctt
     1261 aacaccggga gcgaccccgc ggtccccccg attccggggc tagccggcac gccgttttgg
     1321 accaaccgtg aagcgataca gacgacccat gtgccggcgt cgctcatcgt cttgggtggc
     1381 ggtgcgatcg gcgtcgagct cgcccaggta ttcgctcgct tcggttcacg cgtgactgtg
     1441 gtcgaggcgg cgccgcggct ggtctacttc gaggagccag acgccagcgc ggcagtggcc
     1501 gaagtcttcg gccgcgaagg catcagcgtg cgcgtcggga cgtcggcgac cggcgtccag
     1561 cacgacggca ccggcttcac cgtcgatctc gccggcggcg acagcgttca cgccgagcag
     1621 ttgctcgtcg cgaccggccg tcgggtcgat ctcgccgcgg tcggactcga cactgtcggc
     1681 gtcgacgtga cagctcgggc ggtcccggtc gactcccgga tgcgcgtagc gccaggggtc
     1741 tgggccatcg gcgacatgac cggcaagggt gctttcaccc acgtctcgat gtaccaagcg
     1801 gagatcgcgg tcgccgacat tctcggcatc agcgggatcg ccgacgccga gtacgccgcc
     1861 ctcccgcgag tgacgttcac cgatcccgaa gtgggctccg tcggcatgac gcaagaacag
     1921 gcaacggcgg ccggaatcga cgtcctggtc ggccgtgccc cggtctcatc gtcggcgcgc
     1981 gggtggatcc acggccccgg caacgacgga ttcatcatgc tcgtcgggga cgcgaatcgc
     2041 ggggtgctgg tcggcgcaac cgtggtcgcg ccgtgcgggg gtgaggtgat gtcggcgctc
     2101 gtggtcgcaa tccacgccga ggtgccgctc gagcggctgc tttcgatgat ctatgcgtat
     2161 ccgacgttcc accgcgggat cctcgacgcc ctgaaggacc tgcgccgccc ggcctaacag
     2221 ggctagtgga gggtgtggat ccgggtaatc ggccaaatcc tgacgaacgc gtggccgaca
     2281 acgctgctct cggggatcgt cccgttgtag cgcgagtccg acgaatccga gcggtggtcg
     2341 cccatcacga acagttggcc cttcggcacg acaaccgggc cgaatgcgta gtggttatct
     2401 tcgaacacgt acggctcgtt ctgcgaaacg ccgttgagtt cgacccggcc ctgcgcgtcg
     2461 cagcactgca ccgtgtcacc gggcaatccg atcacccgct taatcacgtc ttcgcggccg
     2521 ccgacaacgg gaatccagtt ccctgtcgcc tgaaagacga cgacgtcgtg ccgatgaatg
     2581 ccgtgcagtt tgtacgacag cttgtcgacg agaactcggt cgtcgttgca gcccggtgtg
     2641 cacccgtgca gcgtcttctc catcgaccct gaagggatgc ggtacgactg caccgcgtac
     2701 gtccgcaaca agaaggtgcc gatcacggtc agcacgatca agatgaccca ctcgcgaccc
     2761 caatggacct tcttcggggt gtccatggga acggtctcgg tggtcaccat gggcggtcac
     2821 gatcggccct ggcgaccggc atcatcagca cggtccgggg tttgcgcgca tcaggactcc
     2881 acgaccggtc acatcaaggt ctgtacccgc gaatgcgaac gccgactcgc cagggcgcaa
     2941 gctgaccgtg ccatcgccgt cgccgacctc gacgctgccc tcagtgcata ggaggacaga
     3001 cgggccagcc ggcgcgagcc gcttggccgt cacgatctgc acgcgccgca actctaactc
     3061 gggctggggc gtcggccagg tctggtcgcc accgtcggtg gccgccgtcg caggcaccag
     3121 cgcaggctcg accgcgccga cgtcgaggat cgcgcgcagg tcggcggctg agacgtgctt
     3181 gggcgtcagc ccaccgcgaa tcacgttgtc ggatcctccg agtatttcga cgccggtacc
     3241 gtgcaggtag gcgtggatct gtcctggacg aacatacaga ccctcgagcg ggcgcaagac
     3301 caggtagttc agcaaggcaa cgcccaccac gcccggatcg ttcgggtacc gcactgcgag
     3361 ccagttgatc cagtgggcga tgccgtccag ggctccggtg gcgctcgcgg cgctggccaa
     3421 gcgcacatcg ctcacgagcc gcaagcggtc gtcagccggc cactcgatca gccgaaggaa
     3481 gacctcgccc gtcggcgtcc cgccttgaag cgcttcgact aaggggtcga ggccaaaaac
     3541 gtcgagcgct ttgagtgcgg ccgcggcgtc gcttgcagca cgaaaaccct gcaatacatg
     3601 gaaatccgtg agcgctacga gcagctcggg cttcggataa ggatcgcgat aacagcgatt
     3661 gggtgcggcc gggtcgatgc ccgcaccttc ttccgcggcg aagccccgct gtgcctgctc
     3721 ggcgttcgga tgcacctgga gcgacagcgg ctgatcggcc gccaacacct tcaaaaggaa
     3781 cggcagccgc ggcccgaagc gttcgacaca acgctcaccc aggacgccga ggggatctgc
     3841 cgcgatcagg gcatcgagag cctcacccgg cgaaccgtcg tcgcgctcga ggagtgacgt
     3901 ggcttggggg tgagcaccca tccacagttc cgcctcggga acatcgccga ccggccggcc
     3961 ctgcagcgtc gcgatcgcct cgcgggagcc ccaggcgtag ggcattacgg ggttcaccat
     4021 gcgccggacc gtcatacctg acgagcctag ggcccgcgcc cgcccgatcg tgggcgcggc
     4081 ttcagtgctg gccggcgccg ttcgatggac aggtatgacg gcggtcacga ctgggagatg
     4141 ggtgtgcccg gcgtgccgtc acgccaatcc ggtcgaggcc gacgtgtgcg ggggctgcgc
     4201 ggtgagcttc accgagctgc tgcgcggttc acgaccacaa cagttggcac cagcgacgcc
     4261 cggaaccctc gacaagatcg cgactgccgt gcgcgagatc ggcatcgtcg ttggcctctt
     4321 cgtactctgg cggctggcga gcgcgatctc gatcggcggc gactccggtg ccttcgcgcg
     4381 cggtcgatgg atctggcggc tcgaacgaac gcttcgactg ccaagcgagc tcaccgtgca
     4441 gaacggattg ctccgccacc acgatttggt gcaggtcgtc aacctgttct acctggccgc
     4501 ccatgtcggc gggcttgtgc ttttcctgcc gtggctgttc attcgccatc gcgagcacta
     4561 ccggcactgg cgcaacgtca tcgccgtgtt caccggcctg tcggtgctga tccaactgct
     4621 ctcagtggca ccgccgcgat tgcttccgca gtacggcttc gtcgatacgg gtgcgctcta
     4681 tcacgagtcg gcctaccagc acctcggcca aggcctgatc ggacagctgt cgtcgatgcc
     4741 gtcgatccac gtgggctggg cgatcgccat cgcgatggcc gtgatctcga ccagcaccag
     4801 ccggtggcgc tggttggtcg tcgcccaccc gatcctgacg gtctacgcgg tcgtcgcgag
     4861 cgccaaccac ttctggctcg actgcgtcgc cgccgccggg ctgctcctgc tggtcattgc
     4921 cggcgtgaac gcgctagcgc gacatcaagc agccaagcga aagccggccg tcacgtaaga
     4981 ccgggtggcg ctgcgtcgag gatctccaga atcgcgtgcg ccaacgccct ggccgagtcg
     5041 gctgtcaact cggccgcgac ccgtgccgcc ggaccgaggc tcggattgcg aaagtcgatg
     5101 ttgagggtgt gcccagcctg cgcgtgcacc ggatggtcga ggtagacgct cgcgtcggtg
     5161 acgttgaacc agtccccttg gcctttggcg ctgccttgtg cggcaagcgt cgccgtcatg
     5221 taggtgcaca tggtcaggcc ccaaggtagc gcgtgaagaa ttcggtgatg cgctgccaac
     5281 cttcgacggc cgccgcaggg cggtagctcg ggcgatcggt ggcgaagaag gcgtgcccag
     5341 catcgtcgta cgaatggaac tcatacgtct tgcccgcgtc gtccagagcc ttcttgagct
     5401 caccaacctc ctcaggtgac gggttcttgt cttcggcacc gaaaagcccg agcagggggc
     5461 agccgagcag cggagcccgt ttgacgatcg gcaaccactt cagccccgat gcctcgggcg
     5521 gcgccgtgac gacaaaggcg ccgtagcaat cgacggcagc gtccaacggc gtactcgtcg
     5581 cggcgagaaa cgactgccgc ccacccgagc agtaaccgat aaccccgact ttcccgttcg
     5641 tataaggcaa agcgcgcagg tgcgccgcag caccggcgac gtcgccgacc agcctggcgt
     5701 cgggaacgcc gcccatcgcg cgcaccatcg ccgcggcgtc gtcaggccgc gcccccggtg
     5761 cttcacgcga atacagattc ggcatcagcg cagcaaatcc gagcgctgcg aaatggcgag
     5821 cgatctcctt cgtcgccgcg tcgtacccgg gcagatgatg aatcaccacc accgacccga
     5881 ccggatccgg accaagcggg cgcgcaaagt aggcctcgat caagtcgccg ccgtcgccgg
     5941 tgacggtggt cgtctccgca aagatcccgt cgtacatcga tgctcctcat cacgtgcgaa
     6001 tcgccacagg aacgacatgg ggccggacgc caagctggcc ggtgtccggt tcacgctacg
     6061 tcgacctgat caaaaccctc acgcgaccac cgagttggcg tcatcatcga cggatgctga
     6121 ggttcagacg gctcggggtc ggtgtcgcca tcggtggcac cgttgccggt cttgggctga
     6181 tgggcctcgt ggcggcagcc gcgcaggcat cggcgagcac gacgatcgac atccgcggat
     6241 cgtggtcgga ggtcacgacg acccagggag cgacctatcc gcagacggtc acgttcgtca
     6301 cagagaactt ctcgaccggg gccgtgaccg gcacggacgt cggcaatggc cagtccttga
     6361 ccgtcaccgg caccatcaag ggctcgacat tgaccacgca cagcgtggag gccggttact
     6421 catctgatgc cgtcggcacc atcaccaaga gcggcgccac gctggcctgg aacggcacgt
     6481 tcaccgacag cgtcgggcac ccgaacggca ccttcaccgc aacgctgacc acgccggcga
     6541 gcggcaccac gtcgacgtcc ggggcgggaa tcctcgacgt ccgcgggctg tgggacggct
     6601 attacatcca gcccggcggc gcgaagtacc acaaccctgt ggtcttcgtg accgagaact
     6661 tcagcaccgg ccagctcacc ggtaccgaca acggcactga cggtgcggcc acaatcaccg
     6721 gcaccatctc tggccgcacg atcaacgcga cgatcgccca accgccgtcc tactcggcca
     6781 acgtcacctg ggtcatctca gccgacggga cgaagctggt ctcgggcggc atcaccgacc
     6841 cgacggcccc gaacgcgttc gtgctcttcc ggcacggtgc accggtcggt gcgggtagcg
     6901 cagtgacgag cggtgccggt gtcacgtcag gacccgcacc catcaccgcc ctgcccaccc
     6961 cctcgcaagc gttctcgtcg cccggccgca tcatcgcgag cgcagccatc agcatcgcgg
     7021 cgctgttgtt catcacgttc ccggcgaacc tgttcaacaa gacgttcgaa gagaactacg
     7081 acgtcatcgc ggcgtggtgg ggcccgcggc tgcgacgctt ccgccgcaag ccttcaaccg
     7141 aggggccgga cgcggagggg ccggcagacg agtcagcgcc cgacactgcc gcaaagaaag
     7201 accggctgaa gttcggcgtc gtcgtcatcg ccggcgcgat cctcggcggc ctgctgaacc
     7261 cgaagttcgg tgcgaacagc gcctcggtga acagcttcat cgcgaccctg ctctcggtca
     7321 tcaccggcac cgtcgtcgga accgcggttg ggttcctcta ccgcaagcgc aagaagcacg
     7381 acctgacccg gcacttcgag gcgctaccag ccggcctggc gatcgcggcc ctgtgcgtcg
     7441 tgctctcgcg agtctccgac ttccagcccg gctatctcta cggcgtcgtg tgcggtgtcg
     7501 cgttcgcggg cagtctggcg aagaacgaga acgggcatct ggtggcgctg ggtcatctgg
     7561 cgacgatcgc cgtcgcagtg atcgcctggt tcctttgggt accggtgaat cacgcggcca
     7621 ttcaccacac cggggtattc ccgctggtga tcctcgacga cctgctcgcg tcgctgttcg
     7681 tcggtggact ggtcggcagc gtgatcaacc tgattccggt gcggttcatg ccggggcatg
     7741 ccttggcaag ctggcaccgc ggcgcgtgga tggcggtgtt cggcgtcgcc gcattcggga
     7801 tgacggagat cgtggtgttc ccgtcgcagc acaatcacgc cgggcacgcg ccgctggcga
     7861 cgatcctggt cttgttcgtg ctgttcggcg gtggctcgct cgcgttccgg gagtacttct
     7921 cgcgccgcga caagcgcctg gaacttgccg cggctccggc cgatggccca ccccccggcg
     7981 tccccactgc ttctgctagc cctgcaccgg aaacatcggg gctgtagtct cccgccgtcg
     8041 ggcctgatgg gctcgcgaga ggagtgcgtg cgtggacgcc accgctgaca cccttcagaa
     8101 ggcgttgcgg atcaacctcg acccgcgctg gtacggcacg atcgccgaga tcggcgccgg
     8161 ccaggaggtg gcccgctggt tctttcgcgc tgggggcgcc gcgggcaccg tcgcgaagtc
     8221 gatctcggcc tacgacatgg cagtcagcga cgcggtctac ggcaagtcag gccggtacgt
     8281 ctcgatgccc cgcctgcaag cgatgctcga cctcgagttc cggctgaaca tcgaacgcct
     8341 cgacgccgag cgcggcgact ccaacgcctt cttcgcgttc gccgacaccg tcacggcccg
     8401 cagttacagc ggcggcaacg agtgtcacgg ctggatgggc gtgaagttcc aagcccagcc
     8461 ccacgacgag ccgagccaga tcatcatgca cgtgcgcatg ttggacgtcg aagccgcgct
     8521 ccagcaggaa gcgctcggca tcgtcggggt gaacttgctg cacgccgcgt tcttccagca
     8581 ccacgagccg gagacgctgg tcgaaagcct ccttgatcgg ctgacaaccg gccgcatcga
     8641 gatcgacatg atcgagctga aggggatcga atttcgcgcg gtcgacaacc ggctcatggc
     8701 cctgaagctg gtgcaggtcg ggctgagcgg cgccgcgatg ttcggccctg accgggaggt
     8761 tctccagccg agcgaggtgc tgcggaaaaa ggcgatcctc gtggagcgcg ggagcttccg
     8821 tccgccgact gtcgtgaaca tcgacatgct cgagtcagcc ttggcgaagt tcgaatccga
     8881 ccctgaggtc agcagccgcg agatcctcat gctgaccgag ttgacgatgg caaacctgct
     8941 cgccggaggc ggcgacgtcg accgccgcga ctttcttgcg cgggccgacc tgcttgcagc
     9001 gtgcggcatg accgtgctga tctccgatta cgtcgagtac caccgtttgg cggcctacct
     9061 cgcgtggcgc acggacgggc gcatcggcat ggtgatggga gtgccgagcc tgatcgatct
     9121 gttcaacgag agcagtcacg cgaagctgcc cggcgggatc ctcgagagct tcggtcggct
     9181 gttcaagaac gatctcaagc tcttcgtcta cccgatgctg cgcgacgacc gggtgacgac
     9241 cgtcgacgac ctggtcgtcg gcggtgacct tcagccgctg tacgactacc tcgcccgacg
     9301 cgggagcttc gtcgggctgg acaactacaa gcccgactac ctgccgatcc tgagccgcga
     9361 cgtgctgcgc cggatcgcga gcggtgacgc gatgtgggaa tcgatggtgc cgcccgaggt
     9421 cggcgaactc atcaagaagc gcgggttctt cggctaccac cga
//
LOCUS       DASWVF010000634         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_502068, whole genome shotgun sequence.
ACCESSION   DASWVF010000634 DASWVF010000000
VERSION     DASWVF010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502068"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGD55_11190"
     CDS             complement(<1..385)
                     /locus_tag="VGD55_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0870954.1"
                     /translation="MADQKVTKAVIPAAGLGTRFLPATKATPKEMLPVVDKPAIQYVV
                     EEAVAAGLDDILMITGRSKRALEDHFDRNYELEDTLRAKGDADRLSLVQEATDLATVH
                     YVRQGEPRGLGHAVLVAAQHVGNEAF"
     gene            complement(451..>1729)
                     /locus_tag="VGD55_11195"
     CDS             complement(451..>1729)
                     /locus_tag="VGD55_11195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0870955.1"
                     /translation="ELSEAAARAASGDLDIAIPVRSKDEVGQLATAFNHMTRELQTYI
                     RQLESSRDELRQNLDRLGATLTHTHDLDGILAVVLDTAIGSAQATGGAIMFLDGDGNL
                     AVQVRRGEVAESVDVGTVVPLGKGISGQVAATGEPVCGRVGDGPGLRRVPSEPDAASV
                     IAVPLKQSGRIVGVLNLYDKEGAREFTSHDLDTILAFSTQAGVAIDNVLLHQEAQRLS
                     LTDPLTGLWNYRYLTIGLGHEIERATRFSRPLAALMLDLDRFKSINDKYGHQVGDAVL
                     IELAGRMRAEVREVDTVSRYGGEEFVIVLPETDAIGAARLAARLGTVIRSTPFCLDQG
                     GNGHAVDPHIGLDVTASIGVAVFPEHGMTPSRLLRSADDALYAAKDAGRDGWRFALAT
                     QDDGTNQERRVLVVPDTDIALPEGADRPRFGDD"
BASE COUNT          266 a          569 c          583 g          311 t
ORIGIN      
        1 cgaacgcctc gttaccgacg tgctgcgccg cgaccagaac ggcgtggccc aatccgcgcg
       61 gttcgccctg acgcacgtag tgcaccgtcg ccaggtccgt cgcctcttgt acgagcgaca
      121 gccggtcggc gtcccctttg gcgcgaagcg tgtcttcgag ttcgtagttg cggtcgaagt
      181 ggtcctcaag tgcgcgcttg ctgcgacccg tgatcatcaa gatgtcgtcg agcccggcgg
      241 cgacagcctc ctcgacgacg tactggatcg ccggcttgtc gacgaccggc aacatctctt
      301 tcggcgtcgc ctttgtggca ggcaggaagc gggtgccgag accggcggcc gggatgacgg
      361 ccttggtgac cttctgatcc gccatgaccg aaccctaacg ttttgggtcg gtcctcgggc
      421 aaaccgggct cggtcgtccc gcgatcaggc tcagtcgtcg ccgaagcggg ggcgatctgc
      481 gccttcaggt agcgcaatat cggtatctgg gacgacgagg actcggcgtt cctggttcgt
      541 gccgtcgtcc tgcgtggcca gtgcaaagcg ccatccgtca cggccggcgt ctttggccgc
      601 atacagagcg tcgtcggcac tgcgcagcaa tcgcgacggc gtcatgccgt gctcggggaa
      661 gacggcgacg ccgatcgagg cggtgacgtc gagtccgatg tggggatcga ccgcgtgacc
      721 gttaccgcct tggtccaggc agaacggcgt cgacctgatg acggtgccga ggcgcgcggc
      781 aagacgcgcg gcgccgatcg catcggtctc ggggagcacg atcacgaact cctcgccgcc
      841 gtagcgcgag acggtgtcga cctcgcggac ttcagcgcgc atccggccgg ccaactcgat
      901 cagcaccgcg tcgccgacct ggtggccgta cttgtcgttg atcgacttga agcggtcgag
      961 gtcgagcatt agcgccgcga gtggtcgact gaatcgcgtg gcgcgctcga tttcgtggcc
     1021 gaggccgatc gtcaggtagc ggtaattcca cagaccggtc agcggatcgg tgaggctgag
     1081 gcgttgcgcc tcttgatgca gcagcacgtt gtcgatggcg acgcctgcct gcgtgctgaa
     1141 cgcgagaatc gtgtcgaggt cgtgcgacgt gaactcgcgc gcgccctcct tgtcgtaaag
     1201 gttcagcaca ccaacgatgc ggcccgactg cttcagcggg acggcgatca ccgacgcggc
     1261 gtccggctcg ctgggcactc gccgcaaccc tggcccatcg ccgacccgcc cgcacaccgg
     1321 ttcacctgtc gcggcaactt ggcctgagat gcccttgccg aggggcacga cggtgcccac
     1381 atcgacgctc tcggcaacct cgccccgacg cacctgcaca gcgagattgc cgtcgccgtc
     1441 gaggaacatg atcgcgccgc cggtcgcctg agcactgccg atcgcggtgt cgaggacgac
     1501 cgcgagaatc ccgtctaggt cgtgcgtatg cgtgagggtc gcgccgagcc ggtcgaggtt
     1561 ttgccgcaac tcgtcgcgac tgctttcgag ttgccggatg taggtctgca actcgcgcgt
     1621 catgtggttg aaggcggtgg ccagctggcc gacctcgtcc ttactccgca ctggaatcgc
     1681 gatgtcgagg tcgccactcg cggcccgcgc ggcagcctcg gacagttcg
//
LOCUS       DASWVF010000635         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_451349, whole genome shotgun sequence.
ACCESSION   DASWVF010000635 DASWVF010000000
VERSION     DASWVF010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451349"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1251
                     /locus_tag="VGD55_11200"
     CDS             85..1251
                     /locus_tag="VGD55_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0870956.1"
                     /translation="MTIKKRAPSGVVDVLRLLVVVFFAGLGYEVSKRFSSGAFELGPL
                     NGVMVGVIIGSGLGYVLGGVLGRTTVTVVGRTEHSLRQTSAEDLVAGSFGGVIGVLVG
                     AGVAWWIFLLLQPFLAFPLFGFVVAVIGYLGYRLGTSRREEMLGMFASRAGMGPRPLA
                     TSNLPRIVDTSVAIDGRVLDVVRAGFLHGTLLVPSPVLAELQGLADAADDIRRSKGRR
                     GLQNLEALKREPGVNVEILEEEVPGVPEVDAKLVRLCLDRGFPLLTLDTNLAKVAALA
                     GVHVMNLHGLALALRPPVSAGDDVVVHLSRPGKEHGQAVGYLDDGTMVVAERSRDHIG
                     DEVAIHVTSVLTTANGRMVFGQPVAAPSAPSAPIPHTPASAVAPTPAAPLGRSL"
     gene            1266..1964
                     /gene="ispD"
                     /locus_tag="VGD55_11205"
     CDS             1266..1964
                     /gene="ispD"
                     /locus_tag="VGD55_11205"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY0870957.1"
                     /translation="MPAAGRGERLGPGQPKALRTLGGVPMLVHAVTALSRARLVDLVV
                     VAAPPDGVAEVEALLALHDIGADLLVVAGGEHRQESVARALEALPPEVDVVLVHDAAR
                     PLAPSELVDAVAGAVLAGHSAVVPGLAMTDTVKRVVTDGQNDQQTEQVVETVQRVDLR
                     CIQTPQGFDRAVLVKAHAAADGFATDDAGLVERLGLPVVVVAGSAEAFKITRPIDLLL
                     AEAVLLERRANGEF"
     gene            1987..2490
                     /gene="ispF"
                     /locus_tag="VGD55_11210"
     CDS             1987..2490
                     /gene="ispF"
                     /locus_tag="VGD55_11210"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY0870958.1"
                     /translation="MTVVLPRVGLGLDVHPFENGRPLWVAGLHWPGETGLAGHSDADV
                     VAHACCDALLSAAGLGDLGTQFGTSDPAWAGASGTALLGETVRRVREGGYEVGNVAVQ
                     LIGNAPRLGPRRDEAQSVLSTAVGGVVSLSATTTDRLGLTGRGEGLAAMATALVYPRA
                     GRPTLAP"
     gene            2487..3890
                     /gene="cysS"
                     /locus_tag="VGD55_11215"
     CDS             2487..3890
                     /gene="cysS"
                     /locus_tag="VGD55_11215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0870959.1"
                     /translation="MTLRIYDTASRQLRDFEPIEPGRVSIYLCGATVQAPPHIGHVRS
                     AVAFDVLARWLSASGHRVVLCRNVTDIDDKILAVAAAAGVPYWEIAQRNQRLFTDAYA
                     ALGCQPPTVEPHATGHVPEMIVLMRRLIEAGHAYAVDGDVYFSVRSFPAYGALSGQDP
                     KNLQQGESDVDTKRDPLDFALWKNAKSGEPSWETPWGSGRPGWHLECSAMATKYLGAR
                     FDIHGGGLDLVFPHHENEQAQSRAAGDGFANFWMHNGLVTLGGDKMSKSLGNTLSVGN
                     LLDQVRPSELRYYLAAPHYRSSVDFTAAALEEAAVAYRRVEGFVERALELVGAGLPSS
                     LASLTLGSFPAAFVDAMNDDIGVPQGLAVLHNTVREGNAALADGDKARVGVALRHVLA
                     MLSVLGLDPVGDAASGVSDLHLVVDRLVALALEQRTAARERKDYAAADAIRDQLAEAG
                     IIVEDTAAGARWQLRRD"
     gene            3893..>4570
                     /locus_tag="VGD55_11220"
     CDS             3893..>4570
                     /locus_tag="VGD55_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0870960.1"
                     /translation="MAGGKRGGQGGRGAKPGSHRKGASVGSGGQKRKGLEGRGPTPPA
                     EERKGHVAQGRARRAAASGRPAASGRPAAGSRSSSSGRPVAGGRPSTARRPTRSTRPD
                     PDAPEIVTGRNPVVEALRAGIPASALYVANGIEVDERVREAIKLANQLRIPLLDAERV
                     ELDRYAQGGVHQGLVLQVPPFDYAHPDELLDRAFSAGVPLLVALDGVTDPRNLGAVVR
                     SVGAFGGH"
BASE COUNT          646 a         1459 c         1621 g          844 t
ORIGIN      
        1 gaggacaagg ccgaagcgat ccttgacgag gtcctcgcct cctaacatgg ggtagagccg
       61 gagtccggcc gcctttggag ggtcatgacc atcaagaagc gcgcccccag tggcgtcgtc
      121 gatgtgctgc gccttcttgt cgtcgtgttc ttcgcggggc tcggctacga ggtgtcgaag
      181 cgcttcagtt ccggcgcgtt cgaactcggc ccgctcaacg gcgtcatggt cggcgtcatc
      241 atcggttcgg gcctcggcta cgtgctcggt ggtgttctgg gccggacgac ggtcactgtc
      301 gtcggtcgca ccgagcacag cttgcgacaa acatcggccg aagacctcgt tgccggatcc
      361 tttggcggtg tcatcggtgt gctcgtcggc gctggagtcg cgtggtggat cttcctgcta
      421 ctccagccat ttctcgcgtt tccgctgttc ggattcgtcg tggccgtgat cggctacctc
      481 ggctaccgtc tcgggacaag ccgtcgcgaa gagatgctcg gaatgttcgc aagccgagcc
      541 ggcatggggc cgagaccatt ggcgacgtcg aacctgccgc gcatcgtcga cacgtccgtc
      601 gcgatcgacg ggcgggtcct cgatgtcgtg cgcgctggct tcctacacgg cacgctgctg
      661 gtgccgagcc cagtgcttgc ggagttgcaa ggcctcgccg acgcagcaga cgacatccgt
      721 cgttcgaagg gtcgccgcgg cttgcagaac ctagaggcgc tgaagcgcga gcccggtgtc
      781 aacgtcgaga tactcgagga ggaggtccct ggcgtgcccg aggtcgacgc caaactcgtc
      841 cgcctctgcc tcgatcgcgg ctttcccctg ctcacactcg acaccaacct tgcgaaggtc
      901 gccgcgctgg ccggcgtcca tgtcatgaat ttgcacggcc tcgcactcgc gctgcggccg
      961 ccggtgtcag cgggtgacga cgtcgtggtt catctctcgc gtccgggcaa ggaacacggt
     1021 caggccgtcg gctacctcga cgacggcacg atggtggtag ccgagcgctc gcgcgaccac
     1081 atcggtgacg aggtggcgat tcacgtcacg agcgtgctca ccaccgcaaa cggtcgcatg
     1141 gtgttcggcc agccggtagc tgcaccaagt gcgccctccg ctccgatacc tcacactccg
     1201 gcttccgcgg ttgcgcctac gccggccgcg ccgttgggtc gatcgctgtg accgttgcgg
     1261 cgatcgtgcc cgcggcgggg aggggtgaac ggctcggtcc tggtcagccg aaggcattgc
     1321 gcacgctggg cggagtcccc atgctggtgc acgcggtgac cgcgctgtcg cgcgctcgtc
     1381 tcgtcgacct cgtcgtggta gctgcgccgc ccgacggagt cgcggaggtc gaggcgttgc
     1441 tcgcgttgca cgacattggc gccgatctgc ttgtggtcgc cgggggagag caccggcagg
     1501 aatctgtcgc ccgggcgctc gaggcgctgc cgcccgaggt tgacgtcgta ctcgtacacg
     1561 atgcggcgcg tccgcttgct ccgagcgaac tcgtcgacgc cgtggcgggt gcggtgctcg
     1621 cagggcacag tgctgtcgtg ccgggtcttg caatgaccga cacggtgaag cgggtcgtca
     1681 cggacggtca aaacgatcag cagaccgagc aggtcgtcga gaccgtgcag cgcgtcgatc
     1741 tgcgatgcat ccagactccg caggggtttg accgcgccgt gttggtgaag gcgcacgccg
     1801 ctgccgacgg ctttgctact gacgacgctg gcctggttga gcgcctgggt cttcccgttg
     1861 tcgttgttgc tggatccgcc gaggcgttca agatcactcg accgatcgac ctgctcctcg
     1921 ccgaagcggt gctgctcgaa cggcgggcga acggtgagtt ctgatctacc tgatgacccc
     1981 cgaccggtga cggtcgtgct gccgcgggtc gggctcgggc tcgacgtcca cccgttcgaa
     2041 aacggtcgcc cgttgtgggt cgccggcctg cactggccgg gtgagacggg gctcgccggg
     2101 cactccgacg ctgatgtcgt cgcgcacgcc tgctgcgacg cgttgctgtc ggccgccggc
     2161 cttggcgacc tgggcacgca gttcggtacc agcgatccgg cgtgggcggg cgcctcgggt
     2221 accgcgcttc tcggggagac cgttcggcgg gtgcgcgaag gcgggtacga ggtcggcaat
     2281 gtcgccgtcc aactgattgg gaatgcgcct cgcctcggcc cacgtcgcga cgaagcgcag
     2341 tcggtgttgt cgaccgcggt tggtggcgtc gtgtctttgt cggcgaccac caccgacagg
     2401 ctcgggctca ccggtcgtgg cgaagggctg gccgcgatgg cgaccgcgtt ggtctatccg
     2461 cgggccggtc gccctactct tgccccgtga cgctgcgcat ctacgatacg gccagccgcc
     2521 agctgcgtga cttcgaaccg atcgagcccg gccgggtctc gatctacctg tgcggtgcga
     2581 ccgtgcaggc gccgccgcac atcggtcacg tccgctcagc ggtggctttc gacgtcctcg
     2641 ctcgatggct ttcagcaagt ggtcatcggg tcgtgttgtg ccgcaatgtc accgacatcg
     2701 acgacaagat cctggccgta gctgcggctg cgggagtgcc gtactgggag atcgcccaac
     2761 gcaatcagcg gcttttcacc gacgcgtatg cggccctggg ctgccagccg ccgaccgtcg
     2821 agccgcacgc gaccggacat gtgcccgaga tgatcgtgct gatgcgccgc ctgatcgagg
     2881 ccggccacgc gtacgcggtc gacggcgacg tctacttctc ggtgcgctca tttcctgcgt
     2941 atggcgcgtt gtcgggacag gacccgaaga acctccagca gggtgagtcg gacgtcgaca
     3001 ccaagcgcga cccgctcgac tttgcgctct ggaagaacgc caagtcgggc gagccgtcgt
     3061 gggaaacgcc gtgggggtcg ggtcgccccg ggtggcatct cgagtgctcg gcgatggcga
     3121 ccaagtacct gggcgcgcga ttcgatattc acggcggcgg cctcgatctg gtgttccccc
     3181 atcacgagaa cgagcaggcg cagtcgcgag cggcgggtga cgggttcgcg aacttctgga
     3241 tgcacaacgg ccttgtgacg ctcggtggcg acaagatgag caagtcgctc ggcaacacgc
     3301 tgtcggtcgg caacttgctc gatcaggtgc ggccctctga gttgcggtat taccttgccg
     3361 cgccgcacta ccggtcgagc gtcgacttca ccgcggcggc gctcgaagag gccgctgttg
     3421 cgtatcgccg ggtcgaaggt ttcgttgaac gagctttgga gcttgtcggc gcgggccttc
     3481 cctcgtcgtt ggcgtcgttg acgctggggt cattccccgc tgccttcgtc gatgcgatga
     3541 acgacgacat tggagtcccg cagggtctgg ccgtgctgca caacacggtg cgcgagggca
     3601 acgcggcttt ggcggacggc gacaaggcgc gcgttggcgt ggcacttcgg cacgttctcg
     3661 cgatgctgtc ggtgctcggg ctcgatccag ttggtgacgc cgcgtccggg gtatccgatc
     3721 tgcatctggt ggttgaccgt ttggtggcct tggcccttga gcagcggacg gcggcgcggg
     3781 agcgcaaaga ctacgcagct gccgacgcga ttcgcgatca actggccgag gcgggcatca
     3841 tcgtggaaga caccgccgcg ggagcccgct ggcagctgcg gagggattga tcatggcggg
     3901 tggaaagcgc ggcggccagg gcgggcgcgg cgccaagccg ggctcgcatc gcaagggcgc
     3961 gtcagtcggc tcgggcggac agaaacgtaa gggtctcgag ggtcgtgggc ctacgccgcc
     4021 ggccgaagag cgcaagggtc atgtggcgca gggtcgggct cggcgcgcgg ctgcctctgg
     4081 tcggccggct gcctctggtc ggcccgcggc tgggtcgcgt tcttcttcga gcggtaggcc
     4141 ggtcgcgggt gggcgaccgt caactgctcg gcgtccaact cgaagcactc gacctgaccc
     4201 agatgctccc gagatcgtca ccgggcgcaa ccccgtcgtc gaggcgctgc gggcggggat
     4261 tccggcgagc gcgctctatg tggcgaacgg catcgaggtc gacgagcggg tgcgtgaggc
     4321 catcaagttg gccaaccagc tccggattcc gctgctcgac gccgaacgtg tcgagctcga
     4381 tcggtacgcg cagggcggtg tgcaccaggg cttggtgttg caagtgccgc cgttcgacta
     4441 cgcgcatccc gacgaattgc tcgaccgcgc gttctcggca ggcgtgccgt tgctggtcgc
     4501 actcgatggc gtgacagatc cgcgcaatct tggcgcggtt gtgcgttcgg tcggcgcgtt
     4561 cggcggtcac
//
LOCUS       DASWVF010000636         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_119746, whole genome shotgun sequence.
ACCESSION   DASWVF010000636 DASWVF010000000
VERSION     DASWVF010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119746"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1648
                     /gene="groL"
                     /locus_tag="VGD55_11225"
     CDS             20..1648
                     /gene="groL"
                     /locus_tag="VGD55_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460405.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0870961.1"
                     /translation="MSAKEIIFSESARQSIASGLNTLANTVKVTLGPRGRNVIIEKSW
                     GSPTVTKDGVTVAKEIELEDKLANMGAQMVKEVASKTSDIAGDGTTTATVLAQAIYAE
                     GSKLVTAGHNPMDIKRGIEAAVIKIVENLKVQAKPTKGKTEIAQVGTISANGEAVIGQ
                     ILAEAMEKVGKEGVITVEEAKSMETTLEVVEGMQFDRGYLSPYFVTDAERMEAVLDDA
                     FILIHEKKLSNMKDLLPVLEQAAKSGRPLVVIAEDIDGEALATLVVNKLRGTLQVCAV
                     KAPGFGDRRSEMLKDIAVLTGGQAVTEDLGLKLENITLKDLGRAKRITVDKDNTTIVE
                     GAGKKVDIEARVKQLRVQIEETTSEYDREKLQERLAKLVGGVAVIKVGAATESEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRALPALDALKFDDDRQYGVKIIRRAVEEPM
                     RQIAANAGVDGSIVVDRVKNGKGAFGFNAATEEYEDLVKAGVIDPTKVVRAALQNAAS
                     VASLMLSTEALIADRPKRKSGAGDASGAGAGGDF"
     gene            1740..2162
                     /locus_tag="VGD55_11230"
     CDS             1740..2162
                     /locus_tag="VGD55_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0870962.1"
                     /translation="MRTTKVKDIMSEVVETLQAGDTLARARRQLELGGIHHLPVVDAD
                     EHVIGLVTYHRLLEACIAPEGAGALVPEATAVETIMQKDVLTVDQETTAAEAARLLDN
                     NRFGSLPVTKDEKLVGIVTAADFVRVALRYLEREVHHM"
     gene            complement(2173..2775)
                     /locus_tag="VGD55_11235"
     CDS             complement(2173..2775)
                     /locus_tag="VGD55_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0870963.1"
                     /translation="MSTGRRRTVLLVDDDSDWRDVLAEHLDAAGFSVAVATDGYVAWR
                     SFLESEPLVVVTDVQMPFMDGRELLANVRARNFRVPVIVVTASPAQAQAQARAAELDD
                     AYVVIAKPADPDQIIATVKAAISHRTSHTPLAKIWTVATSLALRNERRPLSAMRSWLW
                     TRRGRAAAVSAIAAVLVVSLVLAVRLRPAHRRLFLLRGTA"
BASE COUNT          618 a          907 c         1026 g          476 t
ORIGIN      
        1 gagactggag ccagaagtca tgtcagccaa agaaatcatt ttttcggaga gcgctcgtca
       61 gagtatcgcg agcggtctca acacgctcgc caacaccgtc aaggtgaccc tggggccgcg
      121 cggccgcaac gtcatcatcg agaagtcctg gggttcgccg acggtgacca aggacggcgt
      181 cacggtggcc aaggagatcg agctcgagga caagctcgcc aacatgggcg cgcaaatggt
      241 gaaggaggtc gcgtcgaaga cctctgacat cgccggtgac ggcaccacca ccgccacggt
      301 gctcgcgcag gcgatctacg cggaggggtc caagctggtc accgccgggc acaacccgat
      361 ggacatcaag cgcggcatcg aggcggcggt catcaagatc gtcgagaacc tcaaggtcca
      421 ggcgaagccc accaagggga agaccgagat cgcccaggtc gggacgatca gcgccaacgg
      481 cgaggccgtg atcggccaga tcctggccga agcgatggag aaggtcggta aggaaggggt
      541 catcaccgtc gaggaagcga agtccatgga gacgacgctg gaagtggtcg aggggatgca
      601 gttcgaccgc gggtaccttt cgccctactt cgtgaccgac gcggagcgga tggaggcggt
      661 gctcgacgat gcattcattc tcatccacga gaagaagctc tcgaacatga aggacctgct
      721 gccggtcctc gagcaggcgg ccaagagcgg tcggccgctg gtcgtcatcg ccgaagacat
      781 cgacggcgag gccctggcga ccctggtggt caataagctg cgcggcactc tgcaggtctg
      841 cgctgtcaag gcaccgggct tcggcgaccg tcgcagcgag atgctgaaag acatcgcggt
      901 gctcacgggt ggacaggccg tcaccgagga tctgggcctg aagctagaga acatcacgct
      961 caaggatctt ggtcgcgcca agcgcatcac cgtcgataag gacaacacca cgatcgtcga
     1021 aggtgccggc aagaaggtgg acatcgaggc ccgcgtcaag cagctccgcg tccagatcga
     1081 ggagaccacg tccgagtacg atcgcgagaa gttgcaggag cggctcgcga agctggtcgg
     1141 cggcgtggcc gtcatcaagg tcggtgctgc gaccgagtct gaaatgaagg aaaagaaggc
     1201 cagggtcgag gatgccttgc acgccacgcg cgcggccgtc gaagagggga tcgttccggg
     1261 cggcggtgtc gcgttgattc gagcgctgcc agctttggac gcgctcaagt tcgacgacga
     1321 ccgtcagtac ggcgtcaaaa tcattcggcg ggccgtcgaa gagccgatgc gccaaatcgc
     1381 cgcgaatgcg ggcgtcgatg gctcaatcgt cgtggatagg gtcaagaacg gcaaaggcgc
     1441 cttcggcttc aatgcggcca cagaggagta cgaggacctg gtcaaggctg gcgtcatcga
     1501 ccccacgaag gttgtgcgcg ccgcactgca gaacgccgcc tcggtcgctt cgctcatgct
     1561 ctcgacggag gcgctcatcg ccgatcgacc caagaggaaa tctggcgctg gcgacgccag
     1621 tggtgccggc gccggcggcg acttctagat cagccggttt cgacgactcg cgccttcacc
     1681 gtaggcgatc caggaagctc gaaggcgagc ggcaggcgcg cttacggtat ggtggttcga
     1741 tgcggaccac gaaggtgaag gacatcatga gcgaggtcgt cgaaacgctg caggcggggg
     1801 acacgctggc aagggcgcgg cggcagctgg agcttggcgg gattcatcac ttgcccgtcg
     1861 tcgacgccga cgaacacgtc atcgggctcg tcacatacca tcgcctcctg gaggcgtgca
     1921 tcgcgccgga gggtgcgggt gcactggtgc cggaagccac ggcggtcgag accatcatgc
     1981 agaaagacgt tctcaccgtc gaccaggaga cgaccgcggc ggaggcagcg cgtttgctgg
     2041 acaacaacag gttcggcagc ctaccggtga cgaaggacga gaagctcgtc gggatcgtta
     2101 ccgcggcaga cttcgtgcgc gtcgcgcttc gatacctcga gcgcgaagtg caccacatgt
     2161 gagcccgctg gattaggcgg tgccgcgcaa gaggaacagc ctgcggtgcg ccggccgcag
     2221 ccggacagcc agaacgaggc tgacgaccag taccgccgcg attgcggaga ctgcggcggc
     2281 ccgtccgcga cgcgtccaca gccatgaacg catggcactg aggggtcgac gctcgttccg
     2341 tagcgcgagc gatgtagcga ccgtccaaat tttggcgagc ggggtgtgcg acgttcggtg
     2401 gcttatggcg gccttcaccg tcgcgatgat ttggtcggga tccgccggtt ttgctatcac
     2461 gacatacgcg tcgtccagct cagccgcgcg cgcttgcgct tgcgcttgcg ctggcgaggc
     2521 ggtcaccacg atgactggaa cacggaaatt tcgggcccgg acattggcca gcagctcgcg
     2581 accgtccatg aacggcatct gcacgtcagt gacgacgact aacggctcag actccaggaa
     2641 actccgccat gcgacgtagc cgtccgtcgc gacagcgaca ctgaatccgg ctgcgtccag
     2701 atgctcggcc aagacgtcgc gccagtccga atcgtcatcg acgaggagta ccgtccggcg
     2761 ccgtcccgtc gacataccat caacgtaagg tcccctgctc aggatccaac ctaaggtgcg
     2821 tgctgttgga ctccggcgtg cggatccgat cgccctggag gctgttcgcc gtaccattcg
     2881 tgcgcaggtt ccttcgcacg cgagcggcag ggctgtggcc ggcgtcaacg atggtcgtga
     2941 cacatacaag aaacacaatg tccggcggtc gcgtcctgct cgtggaggac gacgagggga
     3001 tccgtcagaa catcgccgag attcttt
//
LOCUS       DASWVF010000637         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_349103, whole genome shotgun sequence.
ACCESSION   DASWVF010000637 DASWVF010000000
VERSION     DASWVF010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349103"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..901)
                     /gene="sufC"
                     /locus_tag="VGD55_11240"
     CDS             complement(101..901)
                     /gene="sufC"
                     /locus_tag="VGD55_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY0870964.1"
                     /translation="MSALVIRDLHVVVNAGTKGETGAIGSGEESREILRGVDLTVNAG
                     ETHAIMGPNGSGKSTLAYSIAGHPKYTITKGSVTLDGHDVLAMKVDERARAGLFLAMQ
                     YPVEVAGVSVSNFLRTAATAIRGEAPKVRTWVGEVKGAMAKLQMDPAFAERNVNEGFS
                     GGEKKRHEILQLELLNPKFAVLDETDSGLDVDALRVVAEGVNRFRAQPDRGVLLITHY
                     TRILRYIEPDFVHVFVDGRIVEEGGRELADRLEAQGYERYANTDAKTA"
     gene            complement(898..1260)
                     /locus_tag="VGD55_11245"
     CDS             complement(898..1260)
                     /locus_tag="VGD55_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0870965.1"
                     /translation="MSRYVKVGEVASLPRPRGVLRVEVEGEPVAIVCTGRDEFHAISD
                     VCSHAEVALSDGEIHGYTVECWLHGSKFDVRTGKPLGPPATRAVPVYDIKFDGDDVYV
                     DVAPSGSPSSQPNQESVR"
     gene            complement(1260..2471)
                     /gene="sufD"
                     /locus_tag="VGD55_11250"
     CDS             complement(1260..2471)
                     /gene="sufD"
                     /locus_tag="VGD55_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0870966.1"
                     /translation="MTELTTTEDLELGELQRAKAELTDAPAAPEGHAHGRGGEGGRHL
                     DRTFSFDPQAFPEPNSRQELWRFAALPRLRPLFAAPDADGSLAFSWASGAPVEVVPIS
                     DSRVGAVHTPFDRLSAIALQKATEALVITVAGELTEPVVVGAKGTGGVSYGHIVVDVA
                     PHATGTIVLDHVGTAQYAENVEIKVGDGAAVTVVSIQDWDDDAVHASAHTALLGRDAR
                     LRHINISFGGSAVRVTPAVRFSGPGADVELLGLFFADAGQHIDNRLLVDHEMPHCKSR
                     VNYRGALQGAGARSVWTGDVIIRATGIGTDTYEVNRNLLLTDGARADSVPNLEILTGE
                     VVGAGHASATGRFDDEQLFYLQARGIPADQARRLIVRGFFADVIERIQVPELVERLLA
                     TVDRELDSSVS"
     gene            complement(2475..>2921)
                     /locus_tag="VGD55_11255"
     CDS             complement(2475..>2921)
                     /locus_tag="VGD55_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY0870967.1"
                     /translation="FAGEGQHQDAGSKMVHAAPHTSSTIISKSVARGGGRTSYRGLVQ
                     IMDGAYGSKSTVKCDALLIDAISRSDTYPYVDVREDDVSMGHEATVSKVSEDQLFYLM
                     SRGMTEDEAMAMIVRGFVEPIARELPMEYALELNRLIELQMEGSVG"
BASE COUNT          490 a          949 c          965 g          517 t
ORIGIN      
        1 gtagatcagc ggtaggccgt catggacgcg ccgctcgagg atcgggaagt ccttgcggac
       61 aagctcgacg tcgtagggtg cgttcatcgg atccacctcc tcacgcggtc ttggcatcgg
      121 tgttggcgta acgctcataa ccctgcgcct cgagccggtc ggccagctcg cggccgccct
      181 cttcgacgat gcggccgtcg acgaacacgt gcacgaaatc gggctcgatg tagcgcagga
      241 tgcgcgtgta gtgcgtgatg agcaggacgc cgcggtcagg ctgtgcacgg aaccggttca
      301 cgccctcggc gaccacccga agtgcgtcga catcaaggcc ggagtcggtc tcgtcgagca
      361 ccgcgaattt cgggttcagc agttcgagtt gaaggatctc gtgacgcttc ttctctccac
      421 cagagaagcc ctcgttcacg ttgcgctcgg caaacgcagg atccatctgc aacttcgcca
      481 tggcgccctt gacctcgccg acccaggtgc gcaccttcgg tgcctcgccg cggatcgccg
      541 tcgccgcggt gcgcaggaag ttcgacaccg agacgcctgc gacctcgacc gggtactgca
      601 ttgcgaggaa cagcccggcc cgcgcgcgct cgtcgacctt catggcgagc acatcgtggc
      661 cgtcgagggt gaccgacccc ttggtgatcg tgtacttggg gtgaccggcg atcgagtacg
      721 ccagtgtcga tttgccagaa ccgttcgggc ccatgatggc gtgcgtctcg ccggcgttca
      781 ccgtgaggtc gacgcctcgc aagatctcgc gggattcttc gccgctgccg atcgcgccgg
      841 tttcaccctt cgtgcctgca ttgacgacga cgtgcaggtc gcggatcaca agcgcgctca
      901 tcgcacggac tcctggtttg gttgagaaga cgggctaccg gatggggcta cgtcgacgta
      961 aacgtcgtcg ccgtcgaact tgatgtcgta caccggcaca gcacgcgttg ccggggggcc
     1021 gaggggtttg ccagtgcgca cgtcgaactt cgaaccgtgc aaccagcact cgacggtgta
     1081 gccatggatc tccccgtcgg atagcgctac ctcggcgtgc gagcacacat cggagattgc
     1141 gtggaattcg tcgcggccgg tgcagacgat cgcgaccggc tcgccctcga cctcgacgcg
     1201 cagcacgccg cgcggacgcg gcaaggaggc gacttcaccg accttcacgt accggctcat
     1261 cacgacacgc tgctatcgag ttcgcggtcg actgtggcga gcaagcgctc gaccaactca
     1321 ggtacttgaa tgcgctcgat gacgtcagcg aagaaccctc gcacgatcag ccggcgggcc
     1381 tggtcggctg gtatcccccg cgcctgcaag tagaaaagct gctcgtcgtc gaaccggccg
     1441 gtggcgctcg cgtggccggc gccgacgacc tcgccggtca agatctcaag gtttggcacg
     1501 gagtcagcgc gggcgccatc ggtgagcaac aggttgcggt tgacctcgta ggtgtcggtg
     1561 ccgatgccgg tcgcgcgaat gatgacgtca ccggtccaga ccgagcgggc accggcacct
     1621 tgcaatgcgc cgcgatagtt gacccgactc ttgcagtgcg gcatctcgtg gtcgacgagc
     1681 aaccggttgt cgatgtgctg acccgcgtcg gcgaagaaca agccgagcaa ctcgacgtcc
     1741 gcccctgggc ctgaaaaacg aacggcgggg gtgacgcgaa cggcgctacc gccgaagctg
     1801 atgttgatgt gccgcagtct cgcgtcgcgg ccgaggaggg cggtgtgagc agacgcgtgc
     1861 accgcatcgt cgtcccagtc ctggatgctc accacggtga ctgcggcgcc gtcgccgact
     1921 ttgatctcga cgttctctgc atactgcgcg gtgccgacgt ggtcgagcac gatcgtgccc
     1981 gtcgcgtgcg gcgcgacatc gacgacgatg tgcccgtacg acaccccacc ggtgcccttg
     2041 gcgccgacca cgaccggttc ggtgagctcg cccgcaacgg tgatgacgag tgcttcggtc
     2101 gccttctgca gggcgatcgc cgacagtcgg tcgaacgggg tgtgcacggc gccgacgcgg
     2161 ctatcgctga tcggcacgac ctcgacaggc gcaccactgg cccatgagaa cgcgagcgat
     2221 ccatcggcat cgggagctgc gaaaagcggc cggagtctgg gcagcgcagc gaaacgccag
     2281 agttcctggc ggctgttggg ctcggggaat gcctgcgggt caaaggaaaa ggtgcggtcg
     2341 agatggcggc cgccttcgcc gcctcgaccg tgcgcatgac cttccggcgc cgcgggtgca
     2401 tcggtgagct ccgctttggc gcgctggagt tcgccgagct cgagatcctc ggtcgtggtg
     2461 agctcggtca tgggtcagcc cacagaccct tccatctgca actcgatcag ccggttcaac
     2521 tcgagggcgt actccatcgg caactcgcgc gcgattggct cgacgaagcc gcgcacgatc
     2581 atcgccatgg cttcgtcctc ggtcatgccc cggctcatca agtagaagag ctgatcttcg
     2641 ctgaccttcg acacagtcgc ttcgtggccc atcgaaacgt cgtcttcgcg gacgtcaacg
     2701 taggggtagg tgtcggagcg gctgatggca tcgatgagga gcgcgtcgca cttcacggtc
     2761 gacttcgacc cgtaggcgcc gtccatgatc tggacgagcc cgcgatagct cgtgcggcca
     2821 ccaccccggg cgacggactt cgagatgatc gtcgacgacg tgtgcggcgc ggcgtgcacc
     2881 atcttcgagc cggcgtcctg gtgctggccc tcgcccgcga a
//
LOCUS       DASWVF010000638         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_400275, whole genome shotgun sequence.
ACCESSION   DASWVF010000638 DASWVF010000000
VERSION     DASWVF010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400275"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGD55_11260"
     CDS             complement(<1..457)
                     /locus_tag="VGD55_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0870968.1"
                     /translation="MKSALRYEWMRLRTLRSTWWLTGLSLLVAGLLGLAAFGIKNPPA
                     GGPQALTITDYGDIITGGGGGAILLVSILLSIIGVFAIGHEYRYGTIRPTLTALPRRS
                     NVLAAKVIVVTAYVFVVALVAVVLRYLVAVAILGHRLLKLGLLPSPIARV"
     gene            complement(454..1365)
                     /locus_tag="VGD55_11265"
     CDS             complement(454..1365)
                     /locus_tag="VGD55_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0870969.1"
                     /translation="MIEAIGLGKRFGDRRAVDNLTFQVQPGVVTGFLGPNGAGKSTTM
                     RLMLDLDHGDGLTRFDGQRYRDIASPMRRVGVLLEAKAFHPTRTARNHLRMLASANSI
                     PVKRVDEVLALVGLESVARKKPKSFSLGMGQRLGLAAALLGEPDILLLDEPANGLDPQ
                     GITWLRDFLKVYAAGGRTVLASSHLLAEMELMADHLIVIGRGKLLADTPIAEFVARSS
                     HTSIVVRSPEAARLSELLVARGATVIPEGDGRLSLTGATQEEVGDLAFAERVPLHELT
                     TRTATLEEAFLEVTAGSQEFETTGGLA"
     gene            1445..2125
                     /locus_tag="VGD55_11270"
     CDS             1445..2125
                     /locus_tag="VGD55_11270"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0870970.1"
                     /translation="MASSRAAKPSSEPELKFASATMHIAVAGVVGVVGAVIMGVTGGW
                     GFAALVGWDVMAAVIVAWIWLTIWPRDPRSTASHAVREDPGRPIVDLLLLGASVASLV
                     GVGFVIAASGNSSGAEKAGYVGFAVVTVFLSWAVVHTAYTLRYARLYYAGKAGGLNFH
                     QIPDREPQYVDFAYISITVGMTFQVSDPEVTSTVMRATILRHALLAYLFGVVIIGVVI
                     NVLAGLTK"
     gene            complement(2141..3100)
                     /locus_tag="VGD55_11275"
     CDS             complement(2141..3100)
                     /locus_tag="VGD55_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0870971.1"
                     /translation="MTTAPVELVTFGETMGVLGTDELGPLRNGHRMVLGIAGAESNVA
                     IGVRRLGHSVAWTGRVGADPIGRLVVRELRAEGVDVSHATTDADAPTGFMLKVRRTTA
                     TSEVIYARKDSAGSRLQRSDLATDLIAAARVLHITGITMALSDSARDCVLAALDVARS
                     HNVRICLDINHRNALWTDDAARSALLDVASRCDFMFASEHEAALLVGALSPTDAARAM
                     ADLGPQHAIIKRGDLGYVASIDGQLHSGAALSVPVADPVGAGDAFVAGYLASWLDGAP
                     TADALRTANCAGAFVVAVSGDWEGLPTRAELDAFGAGGDPVAR"
     gene            complement(3111..3776)
                     /locus_tag="VGD55_11280"
     CDS             complement(3111..3776)
                     /locus_tag="VGD55_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0870972.1"
                     /translation="MSDRLEVPSALLATGVVAIARAASASRVEAAVEALVGAGVTCIE
                     VPLTTPGAVATIASLVTTYAGSACIGAGTVLTTEQASACIDAGAAFLVSPSAVPEVVK
                     LAAARGVPSMPGALTPTEIVAAWASGAAAVKLFPASLGGVRYLRDVRAPLPDIALLPT
                     GGIAIDDVTAYVDAGAIAIGVGGPLFGDALDGGDLDALAARGRRFLAVVAEARQARGT
                     AVR"
     gene            complement(3794..>4403)
                     /locus_tag="VGD55_11285"
     CDS             complement(3794..>4403)
                     /locus_tag="VGD55_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HEY0870973.1"
                     /translation="DEATLGQVRAFVEALARTLVDLDADGLLDDRSPYFVLTCGGSEQ
                     LDVVTSVLRQPLACSRPVRAVLRSGAYVTHDHGLYAATSPLASQLQPAIEVWCQVLSR
                     PEPTLALLGAGRRDVSFDAGLPVALWRRSVRGGEVVPFHATVSKLNDQHAFLDLDAGD
                     VLEVGDLVGLGISHPCTTHDKWQLMPLLDDERQVIDCVRTYF"
BASE COUNT          770 a         1480 c         1463 g          690 t
ORIGIN      
        1 acacgcgcgc aatcggcgac ggcaacaggc caagtttgag cagccgatgg cccaagatcg
       61 ccaccgcgac caggtagcga agcacgacgg ctaccagcgc cacgacgaag acgtaggccg
      121 ttacgacgat gactttcgcc gcgagcacgt tcgagcgcct cggtagtgct gtcaacgtcg
      181 gccggatcgt gccgtagcgg tactcgtggc cgatagcgaa gacaccgatg atcgacagca
      241 gaatcgacac gaggaggatc gcgccgccgc cgccgccggt gatgatgtcg ccgtagtcgg
      301 tgatggtgag tgcctggggg ccaccggccg gtgggttctt gatgccaaac gccgcaagac
      361 cgagcaggcc cgcgaccagc agcgacaagc ccgtcagcca ccacgtcgag cgcagcgtac
      421 gaaggcgcat ccattcgtag cgaagggcgc tcttcatgcg agccctccag tcgtctcgaa
      481 ctcctgcgaa ccggcagtca cttcgaggaa agcctcctcg agcgtggccg tgcgggtggt
      541 gagctcgtgc agcggtaccc gctcggcgaa cgcgaggtcg ccgacctcct cctgcgttgc
      601 gcccgtcagc gacagtcggc cgtcaccttc ggggatcacg gttgcgccgc gtgcgacgag
      661 caactcgctc aacctggccg cctcgggact gcgcacgacg atgctcgtgt gcgatgacct
      721 ggccacgaac tcggcgatcg gagtgtcggc gaggagcttg ccgcgaccga tgacgatcag
      781 atggtcggcc atcaattcca tctccgcgag gaggtggctc gacgcgagca ccgtgcgacc
      841 gcccgcggcg tacaccttca ggaagtcgcg gagccaggtg atgccttgcg gatcgaggcc
      901 gttggcgggt tcgtcgagga gcaagatgtc gggttccccg agcagcgccg ccgccagacc
      961 gagccgctgc cccatgccga gagagaacga cttaggcttc ttgcgcgcga ccgattcgag
     1021 gccaacgagc gccagcacct catcgacgcg cttgaccggg atcgagttcg ccgacgcgag
     1081 catgcgcaag tgatttcgag ctgtccgcgt cggatggaac gccttggctt cgagcagcac
     1141 accgacgcgg cgcatcgggc tcgcgatgtc gcgatagcgc tgaccgtcga atcgcgtgag
     1201 gccgtcgccg tggtcgaggt cgagcatcag gcgcatcgtc gtcgacttcc cggccccgtt
     1261 cggcccgagg aagccggtaa cgacgccagg ctgcacctgg aaggtcaggt tgtcgaccgc
     1321 acggcggtca ccgaaacgct tgccgagccc gatcgcttcg atcacggcta ctcccctgtg
     1381 gatcggacct ggtgatgccc ggcccagcag agtgtagagg gggcaggagt gccacgatgg
     1441 cgacatggca tctagcagag cggcgaaacc gtccagcgaa cccgagctga agttcgcgtc
     1501 ggccaccatg cacatcgcag tggccggcgt cgtgggcgtc gtgggcgccg tcatcatggg
     1561 cgtcacgggc ggctgggggt tcgcggcgct ggtcggctgg gacgtgatgg ccgcggtcat
     1621 cgtggcctgg atatggctga ccatctggcc acgagacccg cgctcaactg caagtcacgc
     1681 cgtgcgcgag gacccgggtc gcccgatcgt cgacctgttg ctcctaggcg ccagcgtcgc
     1741 cagcctggtg ggggtcggtt tcgtcatcgc ggcgagcggt aatagcagcg gcgccgaaaa
     1801 ggccggctac gtggggttcg ccgttgtgac cgtttttctt tcatgggccg tcgtccacac
     1861 cgcctacaca ttgcgctacg ccaggttgta ctacgcaggt aaggcgggcg gcctcaactt
     1921 ccaccagatc cccgaccgcg agccgcaata cgtcgacttc gcctacatct cgataacggt
     1981 cggcatgacc tttcaagttt cggatccgga ggtgacctcg acagtcatgc gggcgacgat
     2041 cctgcggcac gcgttgctgg cgtacctatt cggagtcgtc atcatcggcg tcgtcatcaa
     2101 cgtgttggcc ggtctcacga agtagcacga cagcgagcag tcagcgggcg acggggtcgc
     2161 ccccggcgcc gaaagcgtcc aactcggcgc gggtcggcag gccttcccaa tcaccactga
     2221 cggcgacgac aaatgctccg gcacagttcg ccgtccgcaa tgcgtctgct gttggtgcgc
     2281 cgtcgagcca cgacgcgagg tagcccgcaa cgaacgcgtc gcccgcaccg actggatcgg
     2341 ccaccggcac cgacaacgcg gcgccggagt gcagttgccc gtcgatgctc gcgacgtaac
     2401 cgaggtcgcc gcgcttgatg atcgcgtgct gcggaccgag atcggccatc gcccgcgccg
     2461 cgtcggtcgg acttagcgct ccgacgagca gcgcggcttc gtgttcggag gcgaacatga
     2521 agtcgcaacg cgatgcgacg tcgagcaacg ccgaacgtgc ggcgtcgtcg gtccacaacg
     2581 cgttgcggtg gttgatgtcg aggcagatgc gtacgttgtg cgatctggcg acgtcgagcg
     2641 ccgcgagcac gcagtcgcgc gcagaatcac tgagcgccat cgtgatcccg gtgatgtgca
     2701 acacccgcgc tgccgcaatc agatcggtcg cgagatcgct tcgttgtagt cgcgagccgg
     2761 cactgtcttt gcgcgcatag atgacttccg atgtcgcggt ggtgcggcgg accttcagca
     2821 tgaatccggt tggtgcgtcg gcatcggttg ttgcgtgcga gacgtcgacg ccttcggcgc
     2881 gcagttcgcg gacgacgagg cggccgatcg ggtcggctcc gacacggcca gtccacgcca
     2941 ccgaatgccc tagacgtcgc actccgatcg cgacgttcga ctctgctccg gcgatgccga
     3001 gcaccatgcg atggccgttg cgcagcggcc cgagttcgtc ggtgccgagc actcccatcg
     3061 tctcgccgaa cgtcaccagc tcgaccggcg cggtcgtcat cgcattgccg tcatcgcact
     3121 gccgtgccgc gagcctgccg cgcctcggcg acgacggcca ggaaacggcg tccccttgct
     3181 gcgagtgcgt cgagatcgcc gccgtcgagc gcgtcaccga acaacggccc gccgactccg
     3241 atcgcgatcg ctccggcgtc gacatacgcg gtcacgtcgt cgatcgcgat gccaccggtc
     3301 ggcagcagcg cgatatcggg cagcggcgcc cgcacatcgc gcaggtaccg cacaccaccc
     3361 aacgacgcag ggaaaagctt cactgccgct gcaccgcttg cccatgcggc cacgatctcg
     3421 gtcggagtca acgctccagg catcgacggg acgccgcggg cagccgcgag tttgacgacc
     3481 tcaggcactg cacttgggga gacaagaaat gccgcgccgg cgtcgatgca cgcgcttgcc
     3541 tgctccgtgg ttagtaccgt gccggcaccg atgcacgccg agcctgcgta cgtcgtcacc
     3601 agcgacgcga tcgtggcgac cgcaccgggc gtcgtcagcg gcacctcgat gcaggtcacg
     3661 cccgcgccga cgagggcctc gactgcagcc tcgaccctgc ttgccgacgc ggcccgcgcg
     3721 atcgcgacga caccggtcgc gaggagtgcg ctcggcacct cgaggcggtc agacatcgcg
     3781 ggccccagaa gccttagaag tacgtgcgca cacagtcgat gacctgtctc tcgtcgtcga
     3841 gcaacggcat gagctgccac ttgtcgtgag tggtacacgg atgcgaaata ccgagaccca
     3901 cgaggtcgcc gacctcgagc acgtccccgg catcgagatc gagaaaagca tgctggtcgt
     3961 tgagcttgct cacggtcgcg tggaagggca cgacctcgcc tcccctgacc gagcgtctcc
     4021 acaacgcgac tggaagcccg gcatcgaacg acacgtcgcg gcgacccgca cccagcagcg
     4081 cgagcgtcgg ctcgggtcgc gaaagaacct ggcaccacac ttcgatagcg ggctgcaact
     4141 gcgaagccag cggggacgtc gcggcgtaca ggccgtggtc gtgcgtcacg tatgccccac
     4201 tgcgcaagac cgcacgcacg ggtcgggagc atgcgagcgg ctgccgcaac accgaggtca
     4261 cgacgtccaa ctgctcgctg ccaccacacg tcagcacgaa gtacggcgag cggtcgtcga
     4321 gcaggccgtc ggcgtcgagg tcgaccagtg tgcgggcaag cgcttcgacg aacgcgcgca
     4381 cctggcccaa cgttgcttcg tcg
//
LOCUS       DASWVF010000639         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_196481, whole genome shotgun sequence.
ACCESSION   DASWVF010000639 DASWVF010000000
VERSION     DASWVF010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196481"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGD55_11290"
     CDS             complement(<1..606)
                     /locus_tag="VGD55_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870974.1"
                     /translation="MSPAGVLRLISVALIGGAAALAVHLPWGSPGWFGLPLLAIAVAI
                     CETALINVTVARQRVTFSLTDGVVAAAFLLAPGWWSVVAVVAGLLPTLILTHKSALKV
                     QYNLTQYFVSTMMGALVVVHLGGGVGAALVGMVVWWFCNQVLSAVPLSIMGGESFIPM
                     IFEDAGLHSVHAAATTSIGLLGAWLAVHAPFGLLGLLVPVLV"
     gene            complement(603..>1928)
                     /locus_tag="VGD55_11295"
     CDS             complement(603..>1928)
                     /locus_tag="VGD55_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0870975.1"
                     /translation="DPASSPAPSPSTDGSTDGSTPPDPSASPSVTPLVAPSVGVIAPS
                     TLGGKAGTPNAGRGVTVALIDTGVADTAALNRASGRLVDAVDTSGLNTPNGQVLENGV
                     FTDGYGHGTFMASLIAGGQVPGSGATGLGVAPGATIDVVKVADADGNTSLAAVLAGLD
                     WVATHSDQVDVANLSLSVDPPASRYGIDPLNFAVTLTRAAGVTVVVAAGNTPGVVGDP
                     GFGPASLTVGAADTTGTTPSVASFSGYANVDGMSKPDVVAAGVNLLGEMPVGSTIDQE
                     FPNARQASGLFRGTGTSQSTAIVSGLAALYLQSHPNASPFDVKSAIRDAASSITSGKT
                     DGQGLVAIPTGHGSGNVGESNLNFRQWISTAPQWGDLFTGGAFDARRWSARRWSASGW
                     DARRWAGVTFDARRWSDVTFDSRLWSARMWGSRMWASRMWSASYWGDST"
BASE COUNT          370 a          646 c          654 g          258 t
ORIGIN      
        1 gacaagtact ggcaccagaa gaccgagcaa cccgaacggc gcgtgcactg caagccatgc
       61 accgagcaac ccgatgctcg tcgtcgctgc cgcatggacg ctgtgcaacc cggcgtcctc
      121 gaaaatcatt ggaatgaagc tctcgccgcc catgatcgac agcgggacgg ccgacaacac
      181 ctggttgcag aaccaccaca ccaccatgcc gacgagcgcg gcgccgacgc cgccaccgag
      241 gtgcacgacg acgagcgcgc ccatcatggt cgagacgaag tactgcgtca ggttgtactg
      301 aactttcagc gccgacttgt gcgtgaggat cagcgtcggg agcagaccag cgacgacggc
      361 caccactgac caccatccgg gcgcgagcaa gaacgcagcc gcgaccacgc cgtcagtcaa
      421 cgagaacgtc acccgctgcc gggcaaccgt cacgttgatc agcgcggttt cacagatggc
      481 aacggcgatg gccagcaacg gcaacccgaa ccagccggga ctgccccagg gcaggtgcac
      541 ggcaagcgca gcagcgccgc caatgagcgc gacgctgatg agccggagca cgcccgctgg
      601 gctcacgtgc tgtcgcccca gtacgacgcc gaccacatgc gcgatgccca catccgcgag
      661 ccccacatgc gcgcgctcca caggcgagag tcaaacgtca cgtccgacca ccggcgagca
      721 tcaaaggtca cgccggccca acggcgggca tcccatccag aggcgctcca gcggcgggcg
      781 ctccagcggc gagcatcgaa cgcgccaccg gtgaacaggt cgccccactg cggagcggtc
      841 gagatccact ggcggaagtt caagttgctc tcgccgacgt tgccagaacc gtgaccggtc
      901 gggatcgcga cgaggccctg accgtcggtc ttacccgacg tgatgctcga agcagcgtcg
      961 cgaatcgccg acttgacgtc gaacgggctg gcgttggggt gcgactggag gtacagcgca
     1021 gccaagccgc tgacgatggc ggtcgactgg ctcgtacccg taccgcggaa gaggccactt
     1081 gcctgccggg cgttcgggaa ctcctggtcg atcgtcgagc cgaccggcat ttcgccgagc
     1141 aggttcacac cggcagcgac gacgtcaggc ttgctcatgc cgtcgacgtt cgcgtagcca
     1201 ctgaaggagg cgacgctagg tgtcgtgccc gtggtgtcgg cagcgccgac ggtcagtgac
     1261 gcaggaccga aaccggggtc acccacgacg ccaggtgtgt tgcccgcggc caccacgacg
     1321 gtgacaccag cagctctggt gagcgtgacg gcgaagttca agggatcgat gccatagcgg
     1381 gacgccgggg ggtcgacgct cagcgacagg ttggcaacgt caacctgatc ggagtgcgtc
     1441 gcgacccagt cgaggccggc aagaacggcg gccaacgacg tgttgccatc ggcgtcggcg
     1501 accttgacga cgtcgatcgt cgcgcctggc gcaacaccga ggccagtggc accactgccg
     1561 ggcacttggc cgccggcgat gagcgacgcc atgaacgtgc cgtggccgta gccgtctgtg
     1621 aagacgccgt tctcaaggac ctgcccgttc ggcgtgttca agccagacgt gtcgaccgcg
     1681 tcgacgagac gaccagaagc gcggttcagt gccgctgtgt cagcaacacc ggtgtcgatg
     1741 agcgcgacag tgacgccgcg acccgcgttc ggcgtgccgg ccttgccacc gagcgtgctc
     1801 ggggcgatga cgccgacgct tggcgcgacg agcggggtga cggacggcga ggcgctgggg
     1861 tccggagggg tggatccgtc agtcgaaccg tccgtgctgg ggctcggcgc gggagagctc
     1921 gccgggtc
//
LOCUS       DASWVF010000640         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_94434, whole genome shotgun sequence.
ACCESSION   DASWVF010000640 DASWVF010000000
VERSION     DASWVF010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94434"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..638
                     /locus_tag="VGD55_11300"
     CDS             51..638
                     /locus_tag="VGD55_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870976.1"
                     /translation="MIGPVRHNRGMGHLSLDQLMQGLDHVRAAPTTQGELAAIVSRPA
                     PGEREVLTEATLDHVVGLVGDNWLARGSKSGPALLDSQVTVMNVRAAELVADGTERMP
                     LAGDQLYLDLDLSVDNLPAGSLIRVGHAVLEVSEKPHLGCAKFVERFGEEAMRFVNSR
                     VGRELRLRGANTRVVVPGVIRVGDIVTRDAPPPSV"
     gene            837..3803
                     /gene="uvrA"
                     /locus_tag="VGD55_11305"
     CDS             837..3803
                     /gene="uvrA"
                     /locus_tag="VGD55_11305"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0870977.1"
                     /translation="MVDRLVIRGAREHNLKDVSLDLPRDALIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTSRNPRSTVGTI
                     TEVYDYLRLLYARIGHPHCPVCGRPISRQTPQQIVDRILELDEGSRFQVLAPLIRGRK
                     GEYADLFGDLQTKGFARVRVDGVVYPLTEPPTLKKQEKHTIEVVVDRLAVKESAKRRL
                     TDSVETALKLAGGLLTLEFIDLPEGDPHRERVFSEHLACTYDDLSFEELEPRSFSFNS
                     PFGACTECTGLGTKLEVDPDLIIADPSLSIAKGVIAPWAGGHGSDYFVRLLESLADTM
                     SFSTATPWNKLPVKVRKAVLYGYDEQVHVRYKNRYNRERSYYTTYEGVIPFIERRHGE
                     VESEWSREKFEGYMREVPCPVCHGKRLKPVSLAVTLDDKSIADVAALPIGECATFLRG
                     LELSPREKQIAERLLKEVNSRLGFLLDVGLHYLSLDRAAGTLAGGEAQRIRLATQIGS
                     GLVGVLYVLDEPSIGLHQRDNHRLIDTLVRLRDLGNTLIVVEHDEDTIRTADWVVDIG
                     PGAGEHGGQVVVSGSLKDLLTSKDSLTGAYLSGRRKIDVPAARRRRDAKRVLTVKGAR
                     EHNLRDVDVSFPLGCFIAVTGVSGSGKSTLVNDILYAALARELYGAKTVPGRHLRITG
                     LDQVDKAIHVDQSPIGRTPRSNAATYTGVFDNIRKLFAETTEAKIRGYLQGRFSFNVK
                     GGRCEACAGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLDVHFKGKTIADVLDMPIE
                     EAAEFFAAIPAIARHLVTLVEVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRT
                     VYVLDEPTTGLHFEDVRKLLGVLQSLVDKGNTVVVIEHNLDVVKTADWVIDMGPEGGS
                     GGGLVVAEGTPEQLAQSEESHTGVFLRHLLAAQPTPAHQSPAGPATRRPREASAQAAP
                     AEPATRRPRKATAARS"
     gene            3860..>4196
                     /locus_tag="VGD55_11310"
     CDS             3860..>4196
                     /locus_tag="VGD55_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0870978.1"
                     /translation="MVDPASYRPATGSIPESPGVYRFRDPQDRVVYVGKAKNLRSRLN
                     SYFTDIEALHPRTQSMVWSASRVDWTVVATEVEALQLEYNWIKEFDPRFNVKYRDDKS
                     YPYLRLVSHP"
BASE COUNT          739 a         1363 c         1357 g          737 t
ORIGIN      
        1 accgctgtac gcatccatgg ccacattgtg cactcgataa gcgctcgcgc atgatcggac
       61 ccgtccgcca caatcgcggc atgggccacc tctcactcga ccagttgatg caggggctcg
      121 accacgttcg cgcggcgccg accacgcagg gcgaactggc ggcgatcgtt tcgcggccgg
      181 cgccggggga gcgggaggtc ctgaccgagg cgacacttga tcacgtcgtc gggttggtcg
      241 gcgacaactg gctcgcgcgc ggcagcaaga gtgggccggc cctgctggac tcgcaggtca
      301 ccgtgatgaa cgtgcgggcg gccgagctcg tcgcggacgg caccgagcgg atgcccctag
      361 cgggcgacca gctgtacctc gacctcgacc tcagcgtcga caacctgccc gcgggaagcc
      421 tgattcgggt ggggcacgcg gtgctagagg tgagtgagaa gccgcacctg ggctgcgcca
      481 aattcgtcga acgcttcggg gaggaggcca tgcggttcgt gaactcgcgg gtaggtcgcg
      541 agctgcggct gcgcggagcg aacacccgag tcgttgttcc gggggtgatc cgcgtcggcg
      601 acattgtgac gcgcgacgca cccccaccct ccgtctgaca aggcggaatc gggagcgcgc
      661 aaccggtgtt gggctcggtg ccggttggtc tcgggagcgc gctcgaaccg ctcccaattc
      721 gagagccggc atttctgtcg gaccccacac ctacgatgga ccgagcccgg tggtttccgg
      781 gttcagcggg gtctagcagg gttcgcgacg agtcgagggg atgggtcagc cgcgtcatgg
      841 tcgatcgtct ggtgatccgc ggcgcccgcg agcacaacct caaggatgtc tcgctcgacc
      901 tgccgcgcga cgccttgatc gtcttcaccg ggctttcggg ttcggggaag tcgagcctcg
      961 cgttcgacac gatcttcgct gagggacagc gccgctacgt cgagtcgctg tcggcgtatg
     1021 cccggcagtt cctcgggcag atggataagc ccgacgtcga cttcatcgag ggcctctcac
     1081 cggctgtttc gatcgaccag aaatcgacct cgcgcaaccc gcggtcgacc gtcggcacga
     1141 ttaccgaggt ctacgactac ctccgcttgc tgtatgcccg tatcgggcac ccgcactgtc
     1201 cggtctgtgg tcgcccgatc tcgcggcaga ccccgcagca gatcgtcgac cgcattctcg
     1261 agctcgacga gggcagccga ttccaggttc tcgcgccgct gatccgcggc cgcaagggcg
     1321 agtacgccga ccttttcggc gacctccaga cgaaggggtt cgcgcgcgtc cgggtcgacg
     1381 gcgtggtcta cccgctgacc gagccgccga cgttgaagaa gcaggagaag cacacgatcg
     1441 aggtagtcgt cgaccgcctt gcggtgaaag agtccgccaa gcgccggctg accgactcgg
     1501 tcgagaccgc gctgaagctc gcgggtggtc tgctgacgct ggaattcatc gacctgcccg
     1561 aaggcgatcc gcaccgcgag cgagtgttct ctgagcatct cgcgtgcacc tacgacgacc
     1621 tgtcgttcga agagctcgag ccacgttcgt tctcgttcaa ctcaccgttc ggcgcgtgca
     1681 ccgaatgcac gggtctcggc accaagctcg aggtcgatcc cgacctgatc atcgccgacc
     1741 cgagtttgtc gatcgcgaag ggtgtcatcg caccttgggc cggcggtcat gggtccgact
     1801 acttcgtccg gctgctcgaa tcgcttgccg acacgatgag cttcagcacc gcaacgccgt
     1861 ggaacaagct gccggtgaag gtgcgtaagg ccgtgctgta cggctacgac gagcaggtcc
     1921 acgtccgcta caagaaccgc tacaaccgcg agcgctcgta ctacaccacc tatgagggcg
     1981 tcatcccgtt catcgaacgc cggcatggcg aggtcgaaag cgagtggagc cgcgagaagt
     2041 tcgagggcta catgcgcgag gtgccatgcc ccgtgtgcca tggcaagcga ctcaagccgg
     2101 tgtcgctcgc ggtcactctc gacgacaaat ccatagcgga cgtcgcggcg ttgccgattg
     2161 gcgaatgcgc caccttcttg cgtggtctcg aactttcccc acgcgagaag cagatcgctg
     2221 agcggttgct gaaagaagtc aactcccgct tgggatttct gctcgacgtc ggcctgcatt
     2281 acctctccct tgatcgcgca gccggcaccc tcgcgggtgg cgaggcgcaa cgcatcaggt
     2341 tggccacgca aatcgggtcg gggctcgtgg gcgtcctgta cgtgcttgac gaaccgagca
     2401 tcggattaca tcaacgcgat aaccaccggc tcatcgacac gttggtgcga ctgcgcgatc
     2461 tcggcaacac gctcatcgtc gtcgagcacg acgaagacac cattcgcacc gccgactggg
     2521 ttgtcgacat cgggcccggc gcaggcgagc acggcggcca ggtcgtcgtg tcgggttcac
     2581 tcaaggattt gcttaccagt aaggattctt tgaccggcgc gtacctgtcg ggtcgccgta
     2641 agatcgacgt gccggcggca cgtcggcgcc gtgatgcgaa gcgggtactc actgtcaagg
     2701 gtgcgcgcga gcacaacctg cgcgatgtcg acgtgagctt cccgttgggc tgcttcatcg
     2761 ccgtcaccgg tgtttccggc tcgggtaagt cgacgttggt caacgacatc ctttacgcgg
     2821 cacttgcgcg cgagctttac ggtgcgaaga ccgtacccgg caggcacctt cgcatcaccg
     2881 gcctcgacca ggtcgacaag gcgattcacg tcgaccagag cccgatcggt cgcactccgc
     2941 gatcaaacgc cgcgacgtat acaggtgtgt tcgacaacat tcgcaagctt ttcgccgaga
     3001 ccaccgaggc gaaaatccgt gggtacttgc agggccggtt ctcgttcaac gtgaagggtg
     3061 gccgctgcga ggcatgcgcc ggcgacggca cgataaagat cgagatgaac ttcctgcccg
     3121 acgtgtacgt gccgtgcgag gtctgtcacg gcgcgcggta caaccgcgag acgctcgacg
     3181 tgcacttcaa gggcaagacc atcgccgacg ttctcgacat gccgatcgag gaagctgccg
     3241 agttcttcgc ggcgatcccg gcgatcgcgc ggcacctcgt caccctggtc gaggttggtc
     3301 tcggatatgt gcggctcggt cagccggcgc cgaccctgtc gggtggtgag gcgcagcgcg
     3361 tcaagctcgc gtcagaactc cagaagcggt cgacaggtcg cacggtctat gtccttgacg
     3421 agccgacaac agggctgcat ttcgaggatg tacgcaaact gctcggcgtc ctgcaatccc
     3481 ttgtcgacaa gggcaacacc gtcgtggtca tcgagcacaa cctcgacgtc gtgaagactg
     3541 ccgactgggt catcgacatg ggtccagagg gcgggtcggg cggcggtctt gtggtcgctg
     3601 aaggcacccc cgagcagctg gcccagagcg aagaaagcca caccggcgtg ttcctgcggc
     3661 acctgctcgc ggctcagccg acccccgcgc atcaatcgcc cgccgggcca gccactcgcc
     3721 gtccgcgaga agcgagtgcg caagctgcac ccgccgagcc cgccactcgc cgtccacgca
     3781 aggcaactgc cgcgcggtcc tgagtgagac ggcctttcgc ggaccgcgat ttgtccgcgc
     3841 cgtcggtacg ctggacaccg tggtagaccc cgcgagttat cggcccgcga cgggttcgat
     3901 tcccgagtcg ccgggcgtgt atcgctttcg cgatccgcaa gaccgggtcg tctacgtcgg
     3961 caaagccaag aacctgcgat cgcggctgaa ctcgtacttc accgacatcg aggcgttgca
     4021 tccgcgcacc cagagcatgg tgtggtcggc aagccgcgtc gactggactg tcgtcgcgac
     4081 agaggtcgag gctctccagc ttgagtacaa ctggatcaag gagttcgacc cgcggttcaa
     4141 cgtgaagtac cgcgacgaca agagctaccc ctacctgcgg ctcgtctcgc acccgt
//
LOCUS       DASWVF010000641         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_273006, whole genome shotgun sequence.
ACCESSION   DASWVF010000641 DASWVF010000000
VERSION     DASWVF010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273006"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VGD55_11315"
     CDS             complement(<1..1355)
                     /locus_tag="VGD55_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0870979.1"
                     /translation="MPNQVDLPALEHEVLRRWRDTKVFERSLAATQGRPQWTFYEGPP
                     TANGMPGTHHVEARVFKDLFPRFKTMKGYHVPRKAGWDCHGLPVELAVEKELGFNGKQ
                     DIEAYGIAEFNAKCRTSVERHVDAFERLTERMGYWVDMSQAYRTMDPSYIESVWWSLK
                     TVFDKGLLVSDYRVAPYCPRCGTPLSDHEVSLGYLDVTDPSVFVRFPVTSGPFAGQAD
                     LLVWTTTPWTLVSNTAVAVHPDVLYDVVSLAATDDAPADRFVIARPLVEKVVGDRPRE
                     VLRSFTGRELEHWSYQRPFDLVDFDGERAHYVVLADYVTTEDGTGLVHQAPAFGADDL
                     AVSRAYRLPVVNPVRPDGTFDESLPLVGGMFFKAADKPLVADLRERGLLFRLTPYEHS
                     YPHCWRCDTALLYYALPSWYIRTTAIKDELLRENETTTWYPETIKHGRYGDWLRNNID
                     WA"
     gene            complement(1375..>1717)
                     /locus_tag="VGD55_11320"
     CDS             complement(1375..>1717)
                     /locus_tag="VGD55_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0870980.1"
                     /translation="GIARQLKARGVRLLALTNWSAETFPRARHRMTFLDEFDGVLVSG
                     TVGLVKPDPAIYRLLVERHDVDVRRCVFIDDSLHNVEAAAILGFDAIHFRSSEQLAGE
                     LRQREMLAPLD"
BASE COUNT          305 a          533 c          567 g          312 t
ORIGIN      
        1 gcccagtcga tgttgttgcg caaccagtcg ccataacggc cgtgcttgat ggtttcgggg
       61 taccacgtcg tggtctcgtt ctcgcgcagc agttcgtctt tgatcgcggt ggtacggatg
      121 taccagctcg gcaacgcgta gtagagcagc gcggtgtcgc aacgccagca atgcggataa
      181 ctgtgttcgt agggtgtgag ccggaaaagc agaccgcgtt cgcgaaggtc ggcgaccagt
      241 ggtttgtcgg cggctttgaa gaacatcccg ccgacgagtg gcaacgactc gtcgaaggtg
      301 ccatcgggcc gcaccgggtt cacaaccggc agtctgtacg cgcggctgac ggcgaggtcg
      361 tcggctccga acgcgggtgc ctggtgcacg agaccggtgc cgtcttctgt cgtgacgtaa
      421 tcagcgagga cgacgtagtg cgcacgttcg ccgtcaaagt cgaccaggtc gaagggtcgt
      481 tggtacgacc aatgctcaag ctcacgtccg gtgaagctgc gcaggacctc acgcggtcga
      541 tccccgacga ccttctcgac cagcggccgc gcgatcacga accggtcggc cggcgcatcg
      601 tcagtcgccg ccaaactcac gacgtcgtag aggacgtcgg gatgcacggc gacggcggta
      661 ttgctgacca gcgtccacgg cgtcgttgtc cacacgagca ggtcggcctg ccccgcgaac
      721 ggccctgatg tcacagggaa ccggacgaag acgctcgggt cggtgacatc gagatagccg
      781 agcgacacct cgtggtcaga cagcggcgtg ccacatcgcg ggcagtacgg cgcgacgcga
      841 tagtccgaga ccaggaggcc cttgtcgaag acggtcttca gcgaccacca cacgctttcg
      901 atgtagctcg ggtccatcgt gcggtaggcc tgcgacatgt cgacccaata gcccatgcgc
      961 tcggtgagcc gctcgaacgc gtcgacgtgc cgctcgaccg acgtccggca tttcgcgttg
     1021 aactccgcga tgccataggc ctcgatgtct tgcttgccgt tgaaaccgag ttccttttcg
     1081 acggcgagct cgacgggcag gccgtgacag tcccagccgg ctttgcgcgg gacgtggtag
     1141 cccttcatgg tcttgaagcg cgggaacaga tccttgaaga cccgggcctc gacgtggtgt
     1201 gtgccgggca tcccgttcgc ggtcggcgga ccttcgtaga aggtccattg cggccgcccc
     1261 tgggtcgccg cgagcgatcg ctcgaagact ttcgtatccc gccatcggcg cagcacctcg
     1321 tgctcgagcg cgggcaggtc gacctggttg ggcatcggcg tgaacatcga agcgtcagtc
     1381 taggggagcg agcatctcgc gctgtcgcag ttcgccggct agctgctccg atgaccggaa
     1441 gtggatggca tcgaatccca agattgccgc ggcctctacg ttgtgcagcg agtcgtcgat
     1501 gaaaacgcat ctgcggacat cgacgtcgtg gcgttcgacc agcagtcgat agatcgccgg
     1561 atccggcttg accagaccca ctgtgcctga cacgagcacg ccgtcgaact cgtcgaggaa
     1621 cgtcatgcga tgacgggctc gcggaaacgt ctcggccgac cagttggtca gggccagcag
     1681 tcgcacgccg cgtgctttca gttgccgcgc gatcccg
//
LOCUS       DASWVF010000642         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_170467, whole genome shotgun sequence.
ACCESSION   DASWVF010000642 DASWVF010000000
VERSION     DASWVF010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170467"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VGD55_11325"
     CDS             <1..510
                     /locus_tag="VGD55_11325"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0870981.1"
                     /translation="AHAGGGYAERRRSCEEAARQLGVPYLRDIGGSDLESALARLDGP
                     DRDVLRRRARHIITENDRVLKTVERLSTGRLRDIGPILTQSHASMRDDFEISVPELDT
                     AVEAALAAGALGARMTGGGFGGSAIALIRAAELDDVSRAVEAAFADHGFGAPQIWSPR
                     ASSGAIRDS"
     gene            799..1689
                     /locus_tag="VGD55_11330"
     CDS             799..1689
                     /locus_tag="VGD55_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0870982.1"
                     /translation="MAGPDRVATSILISQTGFPTNLSASAVVLARADDFADALAGSPL
                     AAHFQGPLLLVPTDGLTPPLRSEIQRVLPVGRTVYLLGGVQAIAPGVATDLQTLGFNP
                     VRLAGVDRYDTAVKIANLLGDPPTLFEADGTNFPDGLSAGPAAALSHGAVLLTSGHVQ
                     APETAVYIAAHPTDVRYAIGGPAAAADPKAQALVGADRYATSIAVARELFASPGVVDI
                     ATGGDFADALSGGPVAGVQGAPMILVAPNGPLPGPVQAYFNRYSNTVLSALLFGGTSA
                     VSTTVAKEIAQALVLVPSSS"
     gene            complement(1706..2098)
                     /locus_tag="VGD55_11335"
     CDS             complement(1706..2098)
                     /locus_tag="VGD55_11335"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEY0870983.1"
                     /translation="MASDPEELLRSIERTREELAHAVDTIAGRLDPKRAAKRGVDAVR
                     DGVTTVVDGARVKLHLGSESGTAARSESATAAAKAAKTASDAAVSAVSAKLAQVTPIA
                     KKVPKPAATGMAALVAFAAIVAVRRHRH"
     gene            complement(2113..3165)
                     /locus_tag="VGD55_11340"
     CDS             complement(2113..3165)
                     /locus_tag="VGD55_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0870984.1"
                     /translation="MSVRALSVVPGKPETAGVSERPDPSPDEGAMLVQGLLVGVCGTD
                     IEIIQDGYGWVPPGQDRLVLGHESLGQVIDAPAGGGFAAGDLVAGIVRRPDPVPCEPC
                     AAGQWDFCRNGQYTERGIKERNGYAATQWRVEPEFAVKLDSRLGDCGVLMEPTSVVSK
                     AWEQSERIAARAPFERKIALVTGAGPIGLLAALLGVQRGYEVHVLDRVEGGPKPAIVH
                     DLGATYHSGDVAALGIKPDIVIECTGYGPLVFELTNVVANDAVVCLAGISGGSREVPT
                     SLNMINKQLVLENIVLFGSVNAARRHYEQAAEALAKADLAWLARLVSRKVPLTNYADA
                     LARNDDDVKVAIDLRS"
     gene            3212..3685
                     /gene="bcp"
                     /locus_tag="VGD55_11345"
     CDS             3212..3685
                     /gene="bcp"
                     /locus_tag="VGD55_11345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY0870985.1"
                     /translation="MSTPVHLSAGDPAPDFTLPDADGRPVSLRDFRGRRVIVYFYPEA
                     MTAGCTTQACDFRDSLSQLDGAGITVLGISPDPVAKLARFREEQDLTFPLLSDPARTT
                     LDAYGAYGEKKLYGKTVVGVIRSTFVVGADGVLEVAQYGVKATGHVAKLRRELEI"
     gene            3696..3768
                     /locus_tag="VGD55_11350"
     tRNA            3696..3768
                     /locus_tag="VGD55_11350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3729..3731,aa:Leu,seq:tag)
     gene            3820..4464
                     /locus_tag="VGD55_11355"
     CDS             3820..4464
                     /locus_tag="VGD55_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0870986.1"
                     /translation="MVTATALGGVLVVALAMVLTPGPNMMYLVSRSITQGRRAGFISL
                     GGVAVGFLFYLTATNLGLSELFVAVPEMYVTVKLAGACYLGWLAFKALRPGGTSFFAP
                     IALPPDSPRRLFTMGLLTNLLNPKAAIMYLSLIPQFERPAAGHLIAQGFILGGVQIVA
                     SLTVNSCIVLAAGSISTFLRGRPAWLRVQRYLMGTVLGALAVKLATDHARPLPA"
     gene            4560..>5027
                     /locus_tag="VGD55_11360"
     CDS             4560..>5027
                     /locus_tag="VGD55_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0870987.1"
                     /translation="MEVLVEAVGLTKHYPARDRHSQGFTAVDGIDFTLRRGELFGFLG
                     PNGAGKSSTMRMLACVSPVTSGTLEVLGMDPAKDGRRIRSRLGVVQQDDALDSELSVR
                     ENLEIYGRYFGLSRSDVAERALELLEFAQLTERSTERVDALSGGMRRRLTIARA"
BASE COUNT          774 a         1706 c         1596 g          951 t
ORIGIN      
        1 gcgcatgccg ggggcggata tgccgagcgg cgacggtcgt gtgaggaggc cgcacgccaa
       61 ctcggcgtgc cctacctacg tgatatcgga ggctccgacc tcgagtcggc gcttgcccgc
      121 ctcgacggcc cggatcgcga cgtgcttcgg cgtcgggcgc ggcacatcat caccgaaaat
      181 gaccgcgtgc tgaaaaccgt cgagcggttg agcaccggcc ggttgcgcga catcgggccg
      241 atcctcacgc agtcgcacgc atcgatgcgc gacgacttcg aaatcagcgt gcccgaactc
      301 gacacagcgg tcgaggcggc gctcgcggcc ggtgccctgg gtgcccgcat gaccggtggc
      361 ggtttcggcg gcagcgccat cgcgctcatt cgcgctgccg aactcgacga cgtgagccgg
      421 gccgtcgagg ctgcgtttgc cgaccatggg ttcggggcac cgcagatttg gtccccgcgc
      481 gcatcgtcgg gggcaattcg ggattcttga cctgcccgac gatccttcga ccgcaccagc
      541 ttgctcttgt cggttagggt gcggcccaag gatcgtcggc gttttggtga tccgaccacc
      601 cacgtctcca gggggatacg actgatgccg ccgtcgcgcc gactccgggc ccggtcagcc
      661 gcagcccgcg tcaccgcggt cgtcgtgctg gtggcggcgg cgtccttggc acctgagagc
      721 ggcgcgctcg catcaacgcc ccgcgcagcc accacaccaa gcccgcggac ctcgaccggt
      781 caaggaacgc tcacgcgcgt ggcggggcct gatcgggttg cgacgtcgat tctgatctcg
      841 cagaccggct tcccgaccaa cctctcagcg tctgcggtgg tgctggcgcg tgccgacgat
      901 ttcgctgacg ctctcgccgg cagtccgcta gcagcacatt ttcagggacc gttgctgctg
      961 gtacccaccg acggcctcac cccgcccctg cgaagcgaaa tccagcgggt gttgcccgtc
     1021 ggccgcaccg tctatctgct gggcggcgtt caagcgatcg caccgggtgt cgccactgac
     1081 ctgcagacgc ttggcttcaa tccggtgcgg cttgcagggg tagatcggta tgacacggcg
     1141 gtcaagattg cgaatctgct cggtgatccg ccgacgttgt tcgaggccga cggcacgaac
     1201 ttccctgacg gcctgtcggc ggggccggcc gccgccctca gccacggagc cgtcctcctc
     1261 acgtcgggtc acgtccaggc ccccgagacc gccgtgtata tcgctgcgca cccgaccgac
     1321 gttcgttatg cgatcggtgg cccggctgct gcagccgatc ccaaagcgca agcccttgtg
     1381 ggcgctgacc gctacgcgac gtcgatcgcg gtcgctcgcg agctgtttgc gtcgcctggc
     1441 gtcgttgaca ttgcaacggg cggcgacttc gccgacgcgc tttcgggtgg gccggtggcg
     1501 ggcgtccaag gcgcgccgat gatcctggta gcgccgaacg ggccactccc gggcccggtg
     1561 caggcgtact tcaaccgcta ctcgaacact gtgctgtctg cgttgctttt cggcggcacg
     1621 tcggcggtca gcacgacggt tgcgaaggag atcgcgcagg cactcgtgct cgtgccgtcg
     1681 tcgtcttagc tgccctcgta gctgcttagt gtcggtggcg gcgcaccgcg acgatcgcgg
     1741 cgaaggcaac cagcgcggcc atgccggtag cggccggttt cgggaccttc tttgcgatgg
     1801 gcgtaacctg cgcgagcttt gcggaaacgg ccgacaccgc ggcgtccgac gcagtctttg
     1861 cagcttttgc tgcagcagtt gctgattcag acctcgctgc ggtgccggac tccgacccga
     1921 gatgaagctt cacccgcgcg ccgtcgacca cggtcgtgac cccgtcgcgc accgcgtcga
     1981 ccccgcgctt cgcggcccgc ttggggtcga gccggccggc gatggtgtcg accgcgtgcg
     2041 cgagttcttc gcgggtgcgc tcgatcgaac gcagcaattc ctcgggatca cttgccacgg
     2101 ttcagactct cctcagcttc gcaggtcgat ggcgaccttg acatcatcgt cgtttctggc
     2161 aagcgcgtcg gcgtagtttg tcagtggcac cttgcggctt accaaccggg cgagccacgc
     2221 cagatcagcc ttcgccaacg cctcggcggc ttgctcgtag tgccggcgcg ctgcattcac
     2281 gctgccgaac aagacgatgt tctcgagcac caactgcttg ttaatcatgt tcaggctcgt
     2341 cggcacctcg cggctgcctc ccgagatgcc ggccaggcag accacggcgt cgttcgcgac
     2401 gacgttggtg agctcgaaca cgagcggacc ataccccgtg cactcgatga caatgtcagg
     2461 cttgatgccg agcgcggcga cgtcgccgga gtggtaggtc gcgccgaggt cgtgcacgat
     2521 tgccggctta ggcccaccct cgacgcgatc gagcacgtgc acctcatacc cccgttgcac
     2581 accgagtagc gccgctagca ggccgatcgg accggctccg gtgacgaggg cgatcttgcg
     2641 ttcgaatggt gcccgcgcag cgatcctctc gctctgctcc cacgccttcg acacgaccga
     2701 cgtcggctcc atcaagacgc cgcagtcacc gagccgcgaa tcgagcttca ccgcaaactc
     2761 gggctcgacc cgccattgcg tagccgcgta accgttgcgt tccttaatgc cgcgctcggt
     2821 gtactggccg ttgcggcaga agtcccactg cccggcggcg cacggttcgc aaggaaccgg
     2881 atcgggacgc cggacgatgc cggccaccag gtcgcccgct gcaaatccac cacctgccgg
     2941 cgcatcgatc acctgcccga gcgactcgtg cccgagcacc aaccggtctt ggccgggcgg
     3001 cacccagccg tagccatcct ggatgatctc gatgtcggtg ccgcagacac cgaccagcag
     3061 cccttgcacg agcatcgcgc cttcgtcggg cgacgggtcg gggcgctcgc tcacccctgc
     3121 ggtctcgggc ttgcccggta ccactgacaa ggctctgacg ctcacgttgc ctccatgatc
     3181 gacgtcccgc cctcagggta gcgttgtgcg tgtgagcaca cccgtacacc tctcggccgg
     3241 cgatccggca cccgatttca ccttgccgga cgccgacggt cgaccggttt ctctcagaga
     3301 ttttcgtggt cgccgcgtca tcgtgtactt ctacccggag gccatgacag ctggctgcac
     3361 gacgcaggcg tgcgacttcc gcgacagctt gagccagctc gatggagccg gcattaccgt
     3421 gctgggcatc tcacccgacc cggtcgcgaa gctggctcgc tttcgcgagg aacaagacct
     3481 cacgttcccg ctgttgtccg accccgcccg aaccacgctt gatgcgtacg gcgcgtatgg
     3541 cgagaagaag ctctatggca agacagttgt cggcgttatt cgatcgacgt tcgtcgtcgg
     3601 cgccgatggg gtgttggagg tcgcgcagta cggcgtgaaa gcaacaggtc acgtcgccaa
     3661 gttgcgcagg gaactcgaga tctagatcag ttctaggggc cgtagcccaa tggcagaggc
     3721 acgcggttta ggtccgcgcc agtgtgagtt cgactctcac cggccctact cacgagtaaa
     3781 gcgatgctcc agaacaactg gctgacatag cctggcgcga tggtaacggc gactgcgctt
     3841 ggtggagttc tggttgtcgc gttggcgatg gtgctgacgc ctggcccgaa catgatgtat
     3901 ctggtgtcac gctcgatcac ccagggccgt cgcgcggggt tcatctcact tggcggcgtc
     3961 gcagtcggct ttctcttcta cttgaccgca acgaacctcg ggttgtcgga gctgttcgtc
     4021 gccgtccccg agatgtacgt cacggtcaag ctcgccgggg cctgctacct cggatggctc
     4081 gcgtttaagg cgcttcggcc aggtgggacg tcattctttg cgccaatcgc gctgccacct
     4141 gattcgccac gtcggctgtt caccatgggc ctgctgacca atcttctgaa ccccaaagcc
     4201 gcaatcatgt acctctcgtt gattcctcag ttcgaacggc cggcggccgg acatctcatc
     4261 gcgcaaggct tcatcttggg cggcgtgcag atcgtggcat cgctgacagt gaactcgtgc
     4321 atcgtgctcg ccgccggctc gatctcgacg ttcttgcgcg gtcgcccggc ctggttgcgc
     4381 gtgcagcggt acctgatggg caccgtgctc ggcgcgctgg cggtgaagtt ggccaccgac
     4441 cacgcacgac cattgcccgc ctagctacgg caaaacgggt tcttccaacg ctctgccgtt
     4501 atctttcatt gcctgcataa ctatttgcgc tcaacgacgg attctcctac gctccgctcg
     4561 tggaggttct ggtcgaggcc gtcgggctga caaagcatta cccggcccgc gatcgccact
     4621 cccaaggctt caccgctgtc gacggcatcg acttcacgct gcgccgaggt gagctgttcg
     4681 gatttctcgg gcccaatgga gccggcaagt catcgacgat gcgcatgctc gcctgtgtgt
     4741 cgcccgtcac cagcggcacg ctcgaggtgc ttggcatgga cccggccaag gacggtcgcc
     4801 ggatccggtc gcggctcggc gtcgtccagc aagacgatgc gctcgactcc gaactcagtg
     4861 tgcgtgagaa ccttgagatc tacggccgct atttcggcct ctcgagatcc gatgtcgccg
     4921 aacgagcact cgagctgctc gagtttgccc agctgactga acggtcaacg gagcgggtcg
     4981 acgcgttgtc gggcggtatg cgccgtcggc tcaccatcgc ccgcgcc
//
LOCUS       DASWVF010000643         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_349318, whole genome shotgun sequence.
ACCESSION   DASWVF010000643 DASWVF010000000
VERSION     DASWVF010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349318"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..2331)
                     /locus_tag="VGD55_11365"
     CDS             complement(214..2331)
                     /locus_tag="VGD55_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870988.1"
                     /translation="MPRTLLSVVVVAILASPVVSVVGCSEQPPTAAVTAGVGSIDVAV
                     VQGGVTLSSVSYSITGPSGFSQMGAINVSASATISTLIGGIPAASGYALRLTSTATDG
                     TTTCGGSASFNVTAGSVSTVSVTLDCHQAARTGSVAINGTVNVCPIADGLSASPADVA
                     MGSSVALLIAAHDPDSGPSPLAYSWAAPSGTFSNPTAANPTFTCTALGPVTVTATVSD
                     GDPTAACAASMSAVVTCEPSTLLVPRSLVVSSSTYDRTVGAAAALTVGTKLAGTATAT
                     TGNNYVTVWNNATVDSSFGVTSPIQLTDLDPTTNQILSRVTVPTDQVVTSFPSKSELG
                     LHVTTDTAGPHLVFVGYGGAGVGALDVSNSDAVPGQDPTNPVTFAFGSSYAFPRTIVS
                     MDGSGHFSYTPTVNYGGNNGRAALLGSNGLYYTVGNANNGSASTFGPNGTNPDVTETT
                     GLEVVNPINGLSSSVSIPINNSAEVNPLIQYTFGTAKPDRPGKDNNYRGIVEFGGALY
                     FTKGSGGNGIQTVYTVPTLPTVANAASTTISIVPGFPTDSAKATGGTFTPFALFFANA
                     TTMYVTDEGTGNTTDASTHAGLEKWSLVNGTWQLDYVLTQGLIGQIDRGLNGADGPWP
                     DVTTVGLRNVTAVVEGDQVTLWATTSTSSASGDNGADPNKVVMITDQLSATTLTDPAA
                     AESFKTVVGPTYGTVYRGVAFVN"
     gene            complement(2592..3677)
                     /locus_tag="VGD55_11370"
     CDS             complement(2592..3677)
                     /locus_tag="VGD55_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0870989.1"
                     /translation="MNLQPKRIGAACLLSLALGVACEPPGSEMLANDQSAGARVDPAQ
                     PYSTDPFARASRRRESPYAIVVLPDTQYYAARYPAVFKAQTTWIVQQRGARAIAFVVH
                     EGDLVDTEDVAQWSVASDSMDILADARVPYVLSMGNHDYAVGPSGWPTGRSTMIDQFF
                     PVARFSGNSWFYGTLDPAHIENSYAVLEVPDTEAHWLVVSLEFGPRDAVVKWADEVLK
                     RHSRLPAIVVTHAYLYDDGTRYDVVRRPDQKWNPHRYPIVTQPGGVNDGEEIWQKLIL
                     PNPNVEFVLCGHVLGKGVARSTSTRPDGTIVHEILANYQTLPIGGGGYLRILTFFPDS
                     RVVHVETYSPYLAVSKTDGANDFYLPY"
BASE COUNT          716 a         1293 c         1296 g          753 t
ORIGIN      
        1 cttctcgcgg ccttctaact gctaccggca tccgtcgcca caccaccgca acggttcgtc
       61 gacagtcgcc cgcgacaact cgggggctgc gaaggtccgc gcgattcgtt ctcacaagcg
      121 cgcccttgac cgcccggcta gatattcgtc gctcctcgcc ccttggcttc gcgattcgcg
      181 tggcagaccg cgcggacgtt ggcacgctct tcgtcagttc acgaacgcga caccgcgata
      241 gacggttcca taagttggac cgacgacagt cttgaacgat tcggctgccg ccggatccgt
      301 tagggtcgtc gcggacagct ggtccgtgat catgacgacc ttgttcgggt cggcgccgtt
      361 gtcacccgag gcgctcgacg tcgaagtggt tgcccacagc gtgacctggt caccctcgac
      421 gacggcggtc acgttgcgga gcccgacggt ggtcacatcc ggccacggac cgtctgcgcc
      481 attcagtccg cgatcaatct gaccgatcag tccctgagtc agcacgtagt ccagctgcca
      541 cgtcccgttg accaggctcc acttctcgag ccctgcatgg gttgacgcgt cggtcgtatt
      601 gcctgtgcct tcgtctgtga cgtacatcgt cgtcgcgttg gcgaagaaca gggcgaaggg
      661 cgtgaaggtg ccccccgtcg ccttggccga gtcagtcggg aacccgggga cgatactgat
      721 ggtcgtgctg gccgcgtttg ccacggtcgg aagcgtcggg acggtgtaga cggtttgaat
      781 cccgttgccg ccgctgccct tggtgaagta aagcgccccg ccgaactcca cgatcccgcg
      841 gtagttgttg tccttgcccg gcctgtcggg cttggcggtt ccgaaggtgt actggatgag
      901 cgggttcacc tcggccgagt tgttgatagg gatggacacg ctcgacgaga ggccattgat
      961 cgggttgacg acctccaggc ccgtcgtctc tgtcacgtcc gggttggtgc cgttgggccc
     1021 gaaggtcgag gcgctgccat tgtttgcgtt ccccaccgtg tagtagagac cgttcgagcc
     1081 aagcagcgcc gcccgcccat tgttcccgcc gtaattcacc gtgggggtgt aagagaagtg
     1141 gccgcttccg tccatcgaca cgatcgtgcg cggaaacgcg tagctcgacc cgaatgcgaa
     1201 cgtcacggga ttcgtgggat cctgtccggg cacggcatcc gagttcgaga cgtccagcgc
     1261 gccgacgccg gcgccgccat agccgacgaa caccaggtgc ggccctgcgg tatccgttgt
     1321 gacatggagg ccgagctcgg actttgaagg gaaactggtg acgacctggt cagtcggcac
     1381 cgtgacgcgg ctcaggatct ggttcgtggt ggggtcgagg tcagtgagct ggatcggcga
     1441 cgtgacaccg aacgagctgt ccaccgtcgc gttattccag accgtgacgt agttgttccc
     1501 ggtcgtggcg gtggcggtgc ccgccagctt cgttccaacc gtgagcgcag cagccgcgcc
     1561 cacagtccgg tcataggtgc tgctgctcac caccaaggag cgtggcacga gcagcgtgct
     1621 cggctcgcag gttaccaccg ccgacatact cgcggcgcag gccgccgtcg gatcgccgtc
     1681 ggacacagtc gccgtcacgg tgacggggcc gagcgccgtg caagtgaacg tcggatttgc
     1741 cgccgtagga ttgctgaaag ttccagaggg cgccgcccaa gagtatgcga ggggagacgg
     1801 accgctgtcc ggatcgtgcg ccgcgatcaa aagtgcgact gaagatccca tcgccacatc
     1861 ggccgggctg gcggaaagac cgtcggcgat cgggcagacg ttgacggtgc cgttgatggc
     1921 caccgaccca gttctggccg cctgatggca gtccagcgtg acgctgacgg tcgacaccga
     1981 cccagccgtg acgttgaagc tggcgcttcc gccacaggtg gtcgtcccgt cggtggccgt
     2041 cgacgtcagc ctgagggcgt agccgcttgc cgcggggatg ccgccgatca gcgtcgaaat
     2101 tgttgcgctc gcgctgacgt tgatcgcccc catctgcgaa aagccgctcg gcccggtgat
     2161 cgagtagctc acggaactca gagtcacacc tccctgcacg accgcgacgt cgatggatcc
     2221 gacgccagcc gtgactgcgg cggtaggggg ttgctcactg caaccgacga cactcacgac
     2281 cggcgacgcc aggatcgcga cgacgaccac gctgaggaga gttcgaggca agatgacctc
     2341 caaaggagcg agaaccaggc accgtgctac aaaagcggat cctgcatgag gaagcgcgac
     2401 gggggcgacg ccgccctcag atccttgaga tccgagcaga ccgcctccgg aactgaacgc
     2461 cccttcctct atcccatttg gagcgccgcg aatgtgtcga cctcgcaaac tctctcgtcg
     2521 cgccggttac cgccttgggt tcatcgtcgt gtctggcagt gccagaccta gcggcggctg
     2581 accctcttcg gtcagtaggg aaggtagaaa tcgttcgcgc cgtctgtctt cgaaacggcg
     2641 aggtaaggag agtaggtctc gacatggacg acccgcgagt ccggaaagaa cgtcaggatc
     2701 cggagatacc cgcccccgcc aatcggcaac gtttgatagt tcgccagaat ctcgtgaacg
     2761 atcgtgccgt ccggcctcgt gctcgtcgag cgagccacgc cttttccgag gacgtggccg
     2821 catagcacga attcgacgtt tgggttcggc aatatcagct tctgccagat ttcttcgccg
     2881 tcgttcacgc cgcccggctg ggtgacgatc gggtagcgat ggggattcca cttctggtct
     2941 ggccggcgaa ccacgtcgta gcgggttccg tcatcgtaaa gatacgcatg ggtgaccacg
     3001 attgcgggta accgggagtg tcgtttgagc acttcgtcgg cccacttcac gaccgcgtca
     3061 cgaggaccaa actcgagcga cacgaccaac cagtgcgcct ccgtatcagg gacctcgagc
     3121 accgcgtagc tattctcgat gtgggcagga tccagcgtcc catagaacca ggagtttccc
     3181 gagaaccgtg caacgggaaa gaactggtcg atcatcgtgc tgcgaccggt cggccaccca
     3241 ctcggcccaa cggcatagtc gtggttgccc atcgataaca cgtagggcac ccgcgcatcc
     3301 gccaggatat ccatgctgtc ggaagcgacc gaccattgcg cgacatcttc cgtgtcgacc
     3361 aggtcgcctt cgtggaccac gaacgcgatg gcgcgcgctc cgcgctgctg gacaatccaa
     3421 gtcgtctgcg ccttgaacac agccgggtat ctcgccgcgt aatactgcgt gtccggaagg
     3481 acaacaatcg cgtacggact ttcacggcga cgagaggcgc gagcgaacgg gtcggtagag
     3541 tatggttgcg cggggtcgac tcgcgccccc gcactctggt cattggccag catctcgctc
     3601 ccgggcggct cgcaagcaac accgagtgcc agggacaaca gacacgcggc gccaatgcgt
     3661 tttggttgaa ggttcattgt cgccgttcgt ccgctccgtc agccccgctg attgggctag
     3721 cggcaccctc gatctatcat ggttccgctt tcgggattgg cgacgcgcga ggccgggcgc
     3781 gcgcgcaatg cgtcggttga cggggaggat gtcggccatc gccgtatcgg gcagtccgag
     3841 tcgaccgtcg acgtcaccac cggacatccc ggctactggc ggttgtcggc gatgatctcg
     3901 acgagcggaa tgcgaacacg ggccacgtcg cagctcaacg atttgacgcg tgactttggc
     3961 gagcatgatg atcgtgctgc ccacgccagg agacatacgg gacccctcgg tcctgcttcg
     4021 tccttggcac ccacgcgacc ttcttacgca ccgccggg
//
LOCUS       DASWVF010000644         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_451628, whole genome shotgun sequence.
ACCESSION   DASWVF010000644 DASWVF010000000
VERSION     DASWVF010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451628"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1124
                     /locus_tag="VGD55_11375"
     CDS             93..1124
                     /locus_tag="VGD55_11375"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0870990.1"
                     /translation="MLHAGIDVHKRCTQICIENEDGTITERRILTERRRLMEEFGGRP
                     QTRILLEAATESEWVARCLEGLGHEVIVGDPNFAPMYAQRSRRIKTDRRDARALFDAC
                     RLGAYRPAYRCSDAHRSVRARVGVRDVLVRTRTKCIAMVRALLRQEGIKPERGSTSSF
                     AQRMRELGLHEPLASDIAPILALLDTLTEKIRAADQEMAKLASEEVPQRLMSVPGVGP
                     VTAVAFVSTLDRPSRFRSAKQVRAYLGLVPREHSSGERSSRGRITKQGGSRMRYLLVQ
                     AAWAVLRSRPTPAAEPLRRWATTVAGRRGKFIAVVGLARRLAGILFALWRDGTSFVVP
                     ASGSREVTA"
     gene            1798..2229
                     /locus_tag="VGD55_11380"
     CDS             1798..2229
                     /locus_tag="VGD55_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870991.1"
                     /translation="MRHRIRIQPYIPPELYRKLRAYSAVHDLTDSAVAEAALSEYLGR
                     DEIEEGLMLRRVTGVDSAITRLQQDVDVLSQTLVALAQYALASAPPVVSPDDARKARR
                     LFAEVLARAAKEMDAGVRLAGLLDRARSAQSHADPATRGER"
     gene            2276..4069
                     /locus_tag="VGD55_11385"
     CDS             2276..4069
                     /locus_tag="VGD55_11385"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HEY0870992.1"
                     /translation="MSDDNTIERSDVFRPRIGRRTRPKPERMPTTFLGQLRGALRRQG
                     FGSTGAARARKGSKRRGTIAVRPPNARSRRCVIKARYVKVTPESYKAAKLHLAYLERE
                     GVERDGSAGNLYGADEGFDAQIFRTPVDGEKRQFRFIVSPEDGERLDLKEFTRGFMRQ
                     VEKDTGRSLIWTAVNHYNTDNPHVHVVVRGVDRDGHDVRIDGRYIAQEMRWRAQEVLT
                     RELGPRSDLEMERQRSVYVGREALTPIDRVLAHYQGADGKVTLERLASGQPTERAACL
                     GRLSTLERLELATREGPVQWQLRTGWQRELDRLGQQAEIANRVYRAIPSSAGQHEHVE
                     PGRAIPRFEGAVRAIGLHDEQTGKLYAVVEAPKGLPRYLQLPPNVAQTVRVGDKLSIG
                     SAPDRWVKPSDERIVQFAQRNQGVYDPLAHQRALEENRQRAQDVSGPTPAELVAANVR
                     RLERLERYGLVTHLPDSRWGVSPDLIGKLEAREQSHPRHALRVEIQRPVPERAIDVGA
                     NDRRSTGEQLAKQLRMTYVESPERLTGHVVACPPNAVGREYVQVVDAARGRFTLVPQP
                     PRASELLGRVVTVVADRDRKLSIRMERDLGR"
BASE COUNT          832 a         1342 c         1551 g          668 t
ORIGIN      
        1 ccgatgtagc ctgagatgga caaacgcaga tgggatgaag cgcgggccac aggcgaacgg
       61 gggtaaacct cgggctcgcc aggaggggaa ccatgcttca cgcgggtatc gacgttcaca
      121 agcgctgcac ccagatctgc atcgaaaacg aggacgggac gatcactgag cgtcggatct
      181 tgaccgagcg ccggcggctg atggaggagt tcggcggccg gccgcagacg cgcatcttgc
      241 tcgaagctgc tacggagagt gagtgggtgg cccgttgcct ggaaggcctc ggccacgagg
      301 tcatcgtcgg tgacccgaac ttcgcgccga tgtacgccca gcgaagccgg cgtatcaaga
      361 cggatcgccg agacgcacgg gcgctgttcg acgcgtgcag attgggtgcc taccgaccgg
      421 cttatcgctg ctcggacgcc caccgcagcg tgcgcgcccg ggtgggcgtg cgcgacgtgc
      481 tggtacgaac ccgcaccaag tgcatcgcca tggtccgagc actcttgcgc caggaaggga
      541 tcaaaccaga gcgcggcagc acctcgtcgt tcgcgcagcg gatgcgcgag ctcggactgc
      601 acgagccgct ggccagcgac atcgcgccga tcctggcgct gctcgacacg ctgacggaaa
      661 agatccgcgc tgccgaccag gagatggcaa agctggcctc ggaggaggtg ccgcagcgac
      721 tcatgtcggt gcccggcgtt ggtccggtca cggctgtggc cttcgtctcg actctggatc
      781 ggccgtcgcg ctttcggagc gcgaagcagg tgcgcgcgta cctcggattg gtgccgcgag
      841 agcacagctc gggagagcgt tcgagtcgag gccgaatcac caaacaaggt ggaagcagga
      901 tgcgttacct gctcgtgcaa gcggcctggg cggtgctccg ctcccgcccc acgccagccg
      961 cagagccgct acgccgatgg gccacgacgg tcgccggtcg gcgcggaaaa ttcatcgccg
     1021 tcgtcgggct tgcccgtcgt ctcgccggga tcctcttcgc cctttggcgt gacggaacgt
     1081 cgttcgtggt gcccgcttcg ggcagtaggg aggtgacggc gtagcccaca aagtttttcg
     1141 acgccgctcc aagtcgccgc ggccgtgatt cggtccggct agggaacggt cttcgacgtc
     1201 ggtacacgac cgatgcaagg tttggtaggt gagcacggaa tcttgcctgg cgcagttgag
     1261 gccgagacgc aggaggtgcc gcccaccaaa gtcggatccc atcttgcgcg agacgcaccg
     1321 gcgtctcact cgtagcgcga aaagaagggt gacctgctat ttcgtgtgcc gccgcgctga
     1381 tcgtccgaga gccgtttcgg gctcgcccgc ggatacgcca ttggtgcggc caacgatcgc
     1441 aactcgtcgc ggggacgctg ttagacacct gattccagtt gacaccatcg cgcgcttcaa
     1501 agaagggcaa gataaaggaa cggagctcac gcccgctaag cgggcgcgct cgttccgaaa
     1561 agtgtagagg ttggcgctgt cgcgcggatg atcgctctca cgtaaggctt cggaatcgtg
     1621 gccgtcgggc agtcattcac cgagtcggcg ggcgcgtgat ctcgacggag agcggcacca
     1681 gggacgattt cgagctcccg gcgcccagtg tggcaacgag agcacccccg tttgcgaaca
     1741 ccaggtagca ggcggcgagg acagttcttg ccggcggggc agcgaccagg agacgccatg
     1801 agacatcgga tccgcatcca gccgtacatc ccgcccgaac tctaccggaa gctccgagcg
     1861 tactccgcgg tgcacgatct caccgacagc gccgtagcag aggcagccct gtcggagtac
     1921 ctcggacgtg acgaaatcga ggaagggctc atgctgcgac gggtgacggg cgtggacagc
     1981 gcgataaccc gactgcaaca ggacgtcgat gtgctgtcgc agacgctcgt ggcgctcgca
     2041 caatacgcgc tcgcgtcggc gccgccagtg gtgtcgccag atgacgcgcg aaaggcgcgt
     2101 cgcctgttcg cagaggttct ggctcgggcc gccaaagaga tggacgcggg cgtgcggttg
     2161 gccggattgc tcgatcgggc acgcagcgcg cagtcgcacg ccgacccagc tacgcgaggt
     2221 gaacggtagg gaggcgaggg cgagacgacg tccctgggga cgcagggatg aacccatgag
     2281 cgacgacaac acgatcgagc gcagcgatgt gtttcgaccc cggatcgggc ggcgcaccag
     2341 gccgaaaccg gagcgcatgc ccacgacatt tcttgggcaa ctccgaggtg cgctgcggcg
     2401 gcaagggttt gggagcaccg gcgcagcacg tgcgcgcaag ggaagtaaac gccggggaac
     2461 catcgccgtt cgtccgccga acgcccgaag tcgacgctgc gtgatcaagg cgcggtacgt
     2521 gaaggtgaca cccgagagtt acaaggcggc gaagctgcac cttgcctact tggaacggga
     2581 gggtgtcgag cgcgatggct cggcggggaa cctctatgga gcagacgagg gcttcgacgc
     2641 gcagatcttc cgcacaccgg tcgacggcga gaagaggcag tttcggttca tcgtctcgcc
     2701 ggaggatggc gagcgactcg acctgaagga gttcacccgc gggttcatgc ggcaggtcga
     2761 gaaggacacc ggccggtcgt tgatctggac ggcggtgaac cactacaaca ccgacaaccc
     2821 ccacgtacac gtcgtcgtgc gcggtgtcga tcgagacggg cacgacgttc ggatcgacgg
     2881 ccgctacatc gctcaggaga tgcgctggcg agcgcaggag gtgctgacgc gcgagctcgg
     2941 accacgatcg gatctggaga tggaacgcca gcgttcggtg tacgtcggga gagaggcact
     3001 gacgccgatc gatcgcgtgc tggcgcacta ccagggcgcc gacggaaagg tgacgctgga
     3061 acggttagcg agcggccagc cgacggagcg cgcggcttgt cttggacggc tgtcgacgct
     3121 ggagcgactt gagctggcga cgcgggaagg tcccgtccaa tggcagctga ggaccggatg
     3181 gcagcgggag ctcgatcggc ttggacaaca ggccgagatc gccaatcgcg tctatcgcgc
     3241 tatccccagc agcgccggac agcacgagca tgtagagccc ggacgggcga tccctcgatt
     3301 cgagggggcc gtccgcgcga tcggcctgca cgacgagcag accggcaagc tgtacgcggt
     3361 cgtcgaagca ccgaagggcc tgccgcgata cctccagtta ccgccgaatg tcgcgcagac
     3421 ggtgagggtt ggggacaagc tgtcgatcgg gagcgcgccc gatcgatggg tcaagccgag
     3481 cgacgagcgg atcgtccagt tcgcacagag aaaccagggt gtctatgacc ctctggcgca
     3541 ccagcgagcg ctcgaggaga accgccagcg cgcccaagac gtcagcggac cgaccccggc
     3601 cgagttggtc gcggcgaatg ttcggcggct ggaacgcctg gaacggtacg gtctggtcac
     3661 gcaccttccg gatagccgct ggggtgtgtc gccggatctg atcgggaagc tcgaagcgcg
     3721 cgagcagagt cacccgaggc atgctctccg ggtcgagatc cagcgaccgg tccccgagcg
     3781 agccatcgac gtcggggcga acgatcggag atcgacaggc gaacaacttg cgaagcaact
     3841 tcgcatgacg tacgtcgaat cccccgaacg cctgacgggc catgtcgtcg cctgtccgcc
     3901 aaacgctgtg ggccgggagt acgtccaggt cgtggacgcg gcacgcggcc gattcacgtt
     3961 ggtcccgcag ccgccccggg catcggagct gttggggcgc gtcgtcaccg tcgtagcaga
     4021 tcgcgatcgc aagctgtcga tccggatgga aagggacctc ggccggtgac cgcaggcggc
     4081 tttcagcagt agggatggga aatcgagggc cacatcgccg gacgagcgct cgcgagtgcg
     4141 aggacacgtt ggcgaaggct ctgcgtcggc cttttcgcat ctcgagaatc gatagccggg
     4201 caacgccgcc gcgaaatttg cgaacaccga tccgagaagg cgggtcgttc ggcggtgcgc
     4261 gctcttgggc gcgcgtcgag cgagtggatc tgagcccgaa ccgtcggcgg ctccctttca
     4321 gagaaggagg actgtcgtga atcgcgcgaa aatcagtcgt cggcgacggg atgccggtgt
     4381 ggctacccaa ccc
//
LOCUS       DASWVF010000645         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_425855, whole genome shotgun sequence.
ACCESSION   DASWVF010000645 DASWVF010000000
VERSION     DASWVF010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425855"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGD55_11390"
     CDS             <1..231
                     /locus_tag="VGD55_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0870993.1"
                     /translation="EAPAFGPSVWGVDGDFAGTTITVFVAVATDLSGARLDGVIGGLP
                     INLDATHASVTGEYEGAPALFPLLLCSPLYFF"
     gene            complement(365..>2734)
                     /locus_tag="VGD55_11395"
     CDS             complement(365..>2734)
                     /locus_tag="VGD55_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY0870994.1"
                     /translation="GQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNYIGTEHILLG
                     LIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKQEATAGGPAEGTQSTSLVLDQ
                     FGRNLTQAAREGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     AIVKGEVPETLKDKQLYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRGDIILFIDEL
                     HTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAALERRFQPIQV
                     AEPSLAHTIEILKGLRDRYESHHRVSITDGALVAAATLADRYISDRFLPDKAIDLIDE
                     AGSRMRIRRMTAPPDLREFDEKIAGVRREKESAIDAQDFEKAASLRDREKQLLAAKTQ
                     REREWKAGDMDVVAEVDEELIAEVLATATGIPVFKLTEEETSRLLRMEDELHKRVIGQ
                     EPAIKALSQTIRRTRAGLKDPKRPGGSFIFAGPSGVGKTELSKTLAEFLFGDEDSLIQ
                     LDMSEFMEKHTVSRLFGSPPGYVGYDEGGQLTEKVRRKPFSVVLFDEVEKAHPDIFNS
                     LLQILEDGRLTDAQGRVVDFKNTVIIMTTNLGTRDISKGFNMGFSKEHDAQGNYERMK
                     AKVNEELKQHFRPEFLNRVDDTIVFHQLTEEEIVTIVDLMIAKVDIRLKDRDMGLELT
                     PDAKKVLAKKGYDPVLGARPLRRTIQREIEDVLSEKILFGELKAGQIVVVGAEGVDTP
                     EAAFTFRGEAKPVLVPDIPPLPETAPAAAPAAAADPAGAPGASPAPAAE"
BASE COUNT          441 a          887 c          873 g          533 t
ORIGIN      
        1 gaggcacccg ccttcgggcc gagtgtttgg ggcgtcgacg gggacttcgc cggcacgaca
       61 atcacggttt tcgtcgcggt agccactgac ctgtccggcg cccgcctcga tggcgtcatc
      121 ggcgggctac cgatcaacct cgatgcaacc cacgcgtcgg tcaccgggga gtacgaaggc
      181 gcaccagcat tgttcccgtt actcctctgc tcgccgctgt acttcttcta gcgggcgcaa
      241 cgagcgtctt gggcatgtgg gcgttcagct gaatgcggcg tgtgcgttcg gcggcgacac
      301 aaaccctcgc ggacatgcgt agggccggca cccgcgaatg ggtgccggcc ctacaacgct
      361 cgaactattc ggcggccggt gctggtgatg cgcctggagc tccggccgga tcggcagcag
      421 cagctggtgc agccgccggt gccgtttctg gaagcggtgg gatgtcgggc acgagaaccg
      481 gcttcgcctc gccgcggaag gtaaacgcgg cttcgggggt gtcgacaccc tcggcgccga
      541 cgaccacgat ctggccagcc ttcaactcgc caaacaggat cttctccgac aacacgtctt
      601 cgatctcacg ctggatcgtg cgacgcaacg ggcgcgcacc aaggaccggg tcgtaaccct
      661 tcttggcgag caccttcttc gcgtcaggcg tgagctcgag gcccatgtcg cgatccttca
      721 gccgtatgtc gaccttcgcg atcatcaggt cgacgatcgt gacgatctct tcctcggtga
      781 gctggtggaa gacgatcgtg tcatcgacac ggttcaagaa ctcgggccgg aagtgctgct
      841 tgagctcctc gttgaccttc gccttcatcc gctcgtagtt gccctgcgcg tcatgctcct
      901 tcgagaagcc catgttgaag cccttggaga tgtcacgcgt gccgagattg gtcgtcatga
      961 tgatgacggt gttcttgaag tcgaccaccc gaccctgagc gtcggtcaag cgaccgtctt
     1021 cgaggatctg caacagcgag ttgaagatgt cggggtgcgc cttctcgacc tcgtcgaaca
     1081 agaccaccga gaacggcttg cgccgcacct tctcggtcag ctgaccgccc tcgtcgtaac
     1141 cgacgtagcc gggaggcgaa ccgaacagtc gcgacacggt gtgcttctcc atgaactcgc
     1201 tcatgtcgag ctggatcaac gagtcctcgt cgccgaacag gaactcggcg agcgtcttcg
     1261 agagctcggt cttaccgaca cctgacgggc cggcgaagat gaacgaaccg ccgggacgct
     1321 tcgggtcttt caggccagca cgcgtacggc gaatcgtctg cgacagcgcc ttgatcgccg
     1381 gctcctggcc gatgacccgc ttgtgcagct cgtcctccat gcgcagcaac cgtgacgtct
     1441 cttcctcggt caacttgaac accgggatgc cggtagcagt cgccagcacc tcggcaatca
     1501 gctcttcgtc gacctcggcg acgacgtcca tgtcgccggc cttccactcc cgctcgcgct
     1561 gcgtcttggc agcgagcagt tgcttctcgc gatcgcgaag cgatgccgcc ttctcgaagt
     1621 cttgtgcgtc gatcgcgctt tccttctcac gacggacacc ggcgatcttc tcgtcgaact
     1681 cgcgaagatc cggcggcgct gtcatgcgac gaatgcgcat gcgcgagccg gcctcgtcga
     1741 tgaggtcgat cgccttgtct ggcaggaacc ggtcgctgat gtagcggtcg gcgagggtcg
     1801 ctgctgcgac gagggcgccg tcggtgatgg acacccggtg gtggctctca tagcggtcgc
     1861 gaagaccctt caggatctcg atggtgtggg cgagcgacgg ctcggccacc tggatcggct
     1921 ggaaccgccg ctcgagggcg gcgtccttct cgaggtgctt gcggtactcg tcgagcgtcg
     1981 tcgcgccgat tgtctggagc tcaccgcgag ccagcatcgg cttcaagatg gacgccgcgt
     2041 cgatcgcgcc ttcagcggca ccggcaccga cgagcgtgtg cagctcgtcg atgaacaaaa
     2101 tgatgtcgcc gcgggtgcgg atctctttga ggaccttctt caaccgctcc tcgaagtcac
     2161 cgcggtagcg gctgccggcg acgagcgcgc caaggtcgag ggtgtacagc tgcttgtcct
     2221 tcagcgtctc gggcacctcg cccttgacga tggcctgcgc gaggccctcg acgatcgcgg
     2281 ttttgccgac gccgggctca ccgatcagaa ccgggttgtt cttggtgcga cgcgacagca
     2341 cctgcatgac gcgctcgatt tccttctcgc gcccgatgac cgggtcgagc tttccttcgc
     2401 gcgcggcctg cgtgaggttg cggccgaact gatccaagac gagggacgtc gactgggtgc
     2461 cctcagcggg accgccggcg gtcgcctctt gcttgccctg gtatccagac agcaactgga
     2521 tgacctgctg gcgcacgcgg ttcaggtcgg cgccgagctt caccagcacc tgcgcggcga
     2581 cgccttcgcc ctcacggata aggccgagca ggatgtgctc ggtaccgatg tagttgtgtc
     2641 caagttgcag tgcctcgcga agcgagagct cgaggacctt cttcgcacgg ggcgtgaaag
     2701 ggatatgacc ggacggcgcc tgctggccct gccc
//
LOCUS       DASWVF010000646         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_349436, whole genome shotgun sequence.
ACCESSION   DASWVF010000646 DASWVF010000000
VERSION     DASWVF010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349436"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1427)
                     /gene="obgE"
                     /locus_tag="VGD55_11400"
     CDS             complement(63..1427)
                     /gene="obgE"
                     /locus_tag="VGD55_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0870995.1"
                     /translation="MPTFVDRVILHASAGNGGNGCASIHREKYKPLGGPDGGEGGRGG
                     NVALVVDTDTTTLLAFHHAPHRRATSGKPGQGSNRDGADGEDMILPVPDGTVVKTEDG
                     VVLADLVGAGSTYIVAQGGRGGLGNAALASPRRKAPGFALLGEPGAVQDVVLELKTVA
                     DVALVGYPNAGKSSLVAALSAARPKIADYPFTTLVPNLGVVQAGESRFTVADVPGLVR
                     GASEGRGLGLEFLRHVERCSALVHVIDCATMEPDRDPVSDLEAIEAELAAYDETDVGG
                     VPLNERPRLVALNKVDVPDARDLAEMVRAEIEARGVKVIIVSAATREGLRELSFAMAA
                     LVEQARAARPSEENTRIVLRPRAVDDSGFTVTKVGTSFVVKGDRPERWIKQTDFTNDE
                     AVGYLADRLNRLGVEAELAKAGAEEGAEVVIGDVAFDWEPGMLAGSEHRIGPRGTDSR
                     LDGR"
     gene            complement(1523..1777)
                     /gene="rpmA"
                     /locus_tag="VGD55_11405"
     CDS             complement(1523..1777)
                     /gene="rpmA"
                     /locus_tag="VGD55_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0870996.1"
                     /translation="MAHKKGASSSRNGRDSNAQFLGVKRYGGQLVNAGEILIRQRGTH
                     HHPGLNVGRGKDDTLFALAQGRVEFGTARGRRVVNIVPGT"
     gene            complement(1787..2101)
                     /gene="rplU"
                     /locus_tag="VGD55_11410"
     CDS             complement(1787..2101)
                     /gene="rplU"
                     /locus_tag="VGD55_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0870997.1"
                     /translation="MYAIVRSGGRQHKVAVGDVVEVDRVEGEPGTSLTLAALLLVDGD
                     AVTSDAKALGNVKVTAEVVDHTKGPKIHILKYKNKTGYRKRQGHRQRHSRLKVTGIET
                     GK"
BASE COUNT          354 a          775 c          732 g          383 t
ORIGIN      
        1 cctgcgcgca cccgcgacga gcggccgggc gacgccttcg ccggttgcgg acgtcaccga
       61 catcaccgac cgtcgaggcg cgagtcggtt ccgcggggtc cgatccgatg ctcagacccg
      121 gccaacatgc cgggctccca gtcgaaagcg acatcgccga tgacgacctc tgcaccctct
      181 tcggcgccgg ccttcgcaag ctccgcttcg acaccgagcc ggttcagccg gtcggccaga
      241 tacccaactg cttcgtcgtt ggtgaagtcg gtctgcttga tccagcgctc aggacgatcg
      301 cccttgacca cgaaactcgt gccgaccttg gtcacggtga agccgctgtc gtcgaccgcg
      361 cgcggccgaa gcacgatgcg ggtgttttct tcgctcggtc gcgcagcccg ggcctgctcg
      421 accaacgcgg ccatcgcgaa cgagagctcg cgcaggcctt cgcgggtcgc cgccgacacg
      481 atgatgacct tgacaccgcg cgcttcgatc tcggcgcgca ccatctcagc aaggtcgcgc
      541 gcatcgggca cgtcgacctt gttcagcgca accagtcgcg gacgctcgtt caacgggaca
      601 ccgccgacat cggtttcgtc gtaggccgcg agctcggctt cgatcgcctc gaggtcgctc
      661 accggatcgc ggtccggttc catcgtggcg cagtcgatga cgtgcaccaa cgcgctgcac
      721 cgctcgacat ggcgcaggaa ctcaagaccc agaccgcggc cctcactcgc gccccgaacc
      781 agcccgggaa cgtcggccac cgtgaagcgc gactcccctg cctgcacgac gccaaggttc
      841 ggcacgaggg tcgtgaaggg atagtcggcg atctttggtc ttgccgccga cagcgccgcg
      901 accagcgacg acttgcccgc gttcggatag ccgacgagcg cgacatcggc gacagtcttc
      961 agttcgagaa cgacatcctg gacggcgcct ggctcgccca gtaacgcgaa gccgggcgcc
     1021 ttgcgacgtg gcgacgcgag cgcggcattg ccgagacccc cacggccgcc ttgcgccacg
     1081 atgtaggtcg atccagcgcc aacgaggtcg gcgagcacga cgccgtcttc ggtcttgacc
     1141 acggtgccgt cgggaacggg caggatcatg tcttcgccgt cggccccgtc gcggttgctg
     1201 ccctggccag gcttgcccga cgttgcccgc cggtgcggtg catggtggaa cgccagcaac
     1261 gttgtggtgt cggtatcgac gaccagcgcg acgttgcccc cgcgaccgcc ttcaccgccg
     1321 tccgggccac cgagcggctt gtacttctca cgatggatcg acgcacaccc gttgccgccg
     1381 ttgccggcac tcgcatgcag gatgacgcgg tcgacgaagg tcggcaccgt gtcctcctgt
     1441 cgtgggctcg tgatccagat atgacacgag ggcggaccga aaacggttcg ccctcgtggt
     1501 cgtgcctagg tgatggaccg ggctacgtcc ccggaacgat gttgaccacg cgacggccgc
     1561 gggcggtacc gaattcgacc cggccctgcg cgagtgcgaa gagcgtgtcg tccttgccac
     1621 gaccgacgtt caagcctggg tggtgatgcg tgccgcgctg ccggatcagg atctcgccag
     1681 cgttgacgag ctgcccgccg tagcgcttga caccgaggaa ctgggcgttg gagtcgcggc
     1741 cgttacggct ggacgacgcg cctttcttgt gggccatgcc agcagtctac tttccggtct
     1801 cgatgccagt gaccttgagg cggctgtggc gctgacggtg gccctgccgc ttgcggtacc
     1861 cggtcttgtt cttgtacttg aggatgtgga tcttgggacc cttggtgtgg tcgaccactt
     1921 cggccgtcac cttgacgttg ccgagagcct tggcgtcgct ggtcacggcg tcaccgtcga
     1981 cgagaagaag cgcggcgagc gtgagtgacg tgcctggctc gccttcgacc cggtcgacct
     2041 cgacgacgtc gccgacggcc accttgtgct gcctgccgcc gctgcgaacg atcgcgtaca
     2101 ccgcggaact cctgactgct cgttgctcgg actggactca ccgcggcagt tcacaactgt
     2161 gaaaggcatg cgggaccggg cgcaatgacg atgcaccgac ccccaagagt acggaccagc
     2221 gcggcagggg gtcaaaccga ggcc
//
LOCUS       DASWVF010000647         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_349450, whole genome shotgun sequence.
ACCESSION   DASWVF010000647 DASWVF010000000
VERSION     DASWVF010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349450"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..507
                     /locus_tag="VGD55_11415"
     CDS             151..507
                     /locus_tag="VGD55_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0870998.1"
                     /translation="MSKSVLELTPIQTVACCSPMTREPLSAGQADQVSTLLKALADPV
                     RLRLMSLVLSHIGGEACVCDLTGAFELSQPTISHHLKVLHDAGLLDRDKRGVWVYYRA
                     RQDALDSIAALISPAA"
     gene            504..1244
                     /locus_tag="VGD55_11420"
     CDS             504..1244
                     /locus_tag="VGD55_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY0870999.1"
                     /translation="MTRPVNAGYLPLARRATAEFIGTAFLVMAVVGSGIAAASLSPAD
                     PGLQLLENSLATGAALTALILALQPVSAAFNPVVTLVERALGAIGTVETAVLIGVQLA
                     GGIAGCVLANLMFDLHAVTLSTHNRSTGGHWLGEVVATLGLLLVIFGAARTGRSNKVA
                     FAVGGYISAAYWFTSSTSFANPAVTVARMFSNSFSGIAPASVFPFVAMQLLGGLIAYA
                     AIRLLYPGVVELADVHERSPHLTLAMKG"
     gene            1248..1679
                     /locus_tag="VGD55_11425"
     CDS             1248..1679
                     /locus_tag="VGD55_11425"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0871000.1"
                     /translation="MSTSVSTPMSIPTVLFACIHNSGRSVAARVLAEHYGHGAVTAIS
                     AGSEPGDTINPAVAKVLAERGLSTDAEVPKLLDFDTVVGADVVITMGCGETCPVAPGK
                     RYEDWPIDDPAGQDMETVRRIVDEIDMRVRALIEKLVESVG"
     gene            1689..2783
                     /locus_tag="VGD55_11430"
     CDS             1689..2783
                     /locus_tag="VGD55_11430"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0871001.1"
                     /translation="MKVVVPRETAEHERRVACVPDTVTRLAAAGLSVTLERGAGVAAG
                     YPDESYVSAGAEIADRAALKLADVVLAVQPLPIADAATLREGALVLSFLQPVAEPELI
                     KTFAAHKVSAMSLERLPRISRAQSMDALSSQSLVIGYRAALIAAERLPRFFPLLMTAA
                     GTVPPAKVLVLGAGVAGLQAIATARRLGAVVSAYDVRTAAAEEVKSLGATFVALDLEA
                     LEGAGGYAREMTDDRAARQRELLTPFLAASDAVITTAAVPGRKAPLLVDTAMVEAMRP
                     GTVIIDIAAESGGNCELSRPGEDVVHNGVLVWGGRNMASGMAFDASRLYARNLANLVL
                     LMTKEGNVVPDLDDEIVRGCLVVHAGEVRS"
     gene            2797..3123
                     /locus_tag="VGD55_11435"
     CDS             2797..3123
                     /locus_tag="VGD55_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY0871002.1"
                     /translation="MNNIQLLTVFMLSMFVGFEVISKVSAVLHTPLMSGANAIHGVIL
                     VGAILITGTASGGVQLTLGLLAVVLATANMVGGFVVTDRMLEMFKGRGKAKAAAAKGP
                     TDGTNA"
     gene            3120..>4064
                     /locus_tag="VGD55_11440"
     CDS             3120..>4064
                     /locus_tag="VGD55_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0871003.1"
                     /translation="MSTATEFAYFVTALCFILALKGLSSPKTARYGNLIGAFGAALAV
                     VVTVSQRHLGHRGPIIAAIVVGAVIAVPAARLVKMTAIPQMVAMFNGVGGAAAMLVAL
                     DEYVRLHSVDSTAAAIAVAFTAIVGSVSFTGSLVTFAKLQELMTGRPILVPGAQVVTP
                     LVGLSAVGCAIGAVATSNVALVVVCTGLSLALGVLFVLPVGGADVPVVISLLNAFTGL
                     TVAASGYLLDNTLLIVAGTLVGASGTMLTQLMARAMGRKITGVLFGAFSGGSSAGSTE
                     EVAATDRGVRSAGADDIAILLAYARKVAIVPGYGLAVAQ"
BASE COUNT          617 a         1302 c         1348 g          797 t
ORIGIN      
        1 cttcagccgc agcaacagcc gccggtttgg cagcagtccg tggtttcgca gcaatcggtc
       61 atggcggcgt cccttctggt tcccacacat agacgtgcgt ctatgtgctc cgaggttgcc
      121 acagacatag gtaggcgtca atatagatac atgtctaagt ctgtgctgga gttgacgccg
      181 atccaaactg tcgcgtgctg ctcgccgatg acccgcgagc cgctctccgc agggcaagcc
      241 gaccaggtgt cgacgttgct gaaggccctc gctgacccgg tgcggctgag gctgatgtcc
      301 ttagttctgt cgcacatcgg cggcgaagcg tgcgtgtgcg acctcaccgg tgccttcgag
      361 ttatcgcagc cgaccatcag ccatcacctc aaggtgctgc acgacgccgg actcctcgac
      421 cgcgacaagc gcggggtgtg ggtgtactac cgggctcgcc aggacgcgct cgattcgatc
      481 gctgcactca tcagtccggc agcgtgaccc gcccagtgaa cgccggctac ctaccgctgg
      541 ctcgacgcgc gaccgccgag ttcatcggaa cggcgttcct cgtcatggcg gtcgttggtt
      601 ctgggatcgc cgctgcgagc ctctcgcccg cagatccagg gctgcagctc ctagagaatt
      661 cgctcgcaac gggcgctgcg ctcacggcac tgatccttgc tttgcaaccg gtctcggcag
      721 ccttcaaccc ggtggtgacg ctggtcgagc gtgcgcttgg ggcgatcgga actgttgaga
      781 ccgcggtgct gatcggcgtc cagcttgcag gtggaatcgc cgggtgcgtg ctcgcgaacc
      841 tgatgttcga cctgcacgca gtaacgcttt cgacccacaa ccgctcgacc ggcgggcact
      901 ggctcggtga agtcgtcgcg acgctcggcc tgctgttagt gattttcggt gcagccagga
      961 cggggcggtc gaacaaggtc gcgtttgccg ttggcggcta catcagcgct gcctactggt
     1021 tcacgagctc gaccagcttc gcgaatcctg ccgtcaccgt cgcccggatg ttctcgaact
     1081 ccttctcggg gatcgcacca gcctcggtat ttccgttcgt ggcgatgcag ctccttggcg
     1141 ggttgatcgc ctatgccgcg atccggctgc tctacccagg cgtggtggag ctcgccgacg
     1201 tccatgagcg ttcaccgcac ctcacactcg caatgaaggg ctgagccatg agcacttctg
     1261 taagcactcc tatgagcatt cctactgtgt tgttcgcctg catccacaac tccggtcgat
     1321 cggttgcggc gcgcgtgctc gctgagcatt acgggcacgg cgccgttact gccatctcgg
     1381 ctgggtcgga gcctggagac accatcaacc ccgcagtggc caaggttttg gcggagcgcg
     1441 gcctctctac ggacgccgaa gttccaaagc tgctcgactt cgacaccgtg gtcggagccg
     1501 acgtcgtcat cacgatgggg tgcggggaaa cctgccccgt cgcgcccggc aagcgctacg
     1561 aggactggcc gattgacgac ccggcaggcc aagacatgga gaccgtgcga cgcatagtcg
     1621 acgagatcga catgcgggtg cgagcgctga tcgagaagct ggtcgaatcc gtgggatagg
     1681 ttgcgggcgt gaaagtcgtg gtgccgcgcg agacggccga gcacgagcgg cgcgtggcgt
     1741 gcgtacccga caccgtgacc cggcttgccg ccgcaggctt gagtgtgacg cttgagcgtg
     1801 gcgcgggcgt tgcggccggt taccccgacg agtcgtatgt gtcagccggt gccgagatcg
     1861 ccgatcgcgc ggcgctgaaa ctggccgacg tggttcttgc agtgcaaccg ctcccgattg
     1921 ccgatgcggc gactctgcgc gagggcgcgc tcgtgctgag tttcctgcag ccggtcgccg
     1981 agccggagct gataaagacc ttcgcggcac acaaggtgag cgcgatgtcg ctcgaacggc
     2041 tgccgcgtat ctcgcgcgcg cagagcatgg acgcgttgtc gtcgcagtcg ctggtcatcg
     2101 gctatcgcgc agcactcatt gcggccgaac gcctgccgcg tttcttcccg ctgctgatga
     2161 ctgccgccgg caccgtgcct cccgccaaag tgctggtgct cggcgcgggg gtcgcgggac
     2221 ttcaggcgat cgcgactgct cgccggcttg gcgcggtggt ctctgcctac gacgtccgga
     2281 cggcggcggc tgaggaagtg aagagcctcg gcgctacgtt cgtcgctctc gacctcgagg
     2341 cactcgaggg cgccggcggt tacgcgcgtg aaatgaccga cgatcgggcc gcccgccaac
     2401 gcgaactcct cacccctttc cttgcggcct ccgatgcggt catcaccacg gcggccgtac
     2461 ccggtcgcaa ggctcccttg ctcgtcgaca ccgcgatggt cgaggcgatg cgcccgggca
     2521 ccgtcatcat cgacatcgcc gccgagtcgg gtggcaactg cgaactgtca cgcccgggtg
     2581 aggacgtcgt gcacaacggc gtgctcgtgt ggggtggacg caacatggcg agcggcatgg
     2641 cgttcgatgc cagccgcctc tacgcgcgca acctcgcgaa cctggtgctg ctcatgacca
     2701 aggaaggcaa tgtcgtgccc gatctcgacg acgaaatcgt gcgtggctgc ctcgtcgtgc
     2761 atgccgggga agtccgctca tgacaggcat cggccgatga acaacatcca actgctgacg
     2821 gtcttcatgc tgtcgatgtt cgtcggcttc gaggtcatct cgaaggtctc cgcggtcttg
     2881 cacactccgc tgatgtcggg cgcgaacgcc attcacggcg tgatcctggt cggcgcgatt
     2941 ctcattaccg ggacggcgag tggcggggtt cagctcaccc tcggcctgtt ggcggtggtg
     3001 ttggccaccg ccaacatggt cgggggattc gtggtcaccg accgcatgct cgagatgttc
     3061 aagggccggg gcaaggcgaa ggcggctgca gccaaaggcc cgaccgacgg gaccaacgcg
     3121 tgagcaccgc gactgagttc gcgtacttcg tcacggccct gtgcttcatc ttggcgttga
     3181 aggggttgtc gtctccgaag accgcccgtt acggcaacct gatcggcgcg ttcggcgcgg
     3241 cgctcgccgt ggtggtcacg gtctcgcagc gccaccttgg ccaccgcggc ccgatcattg
     3301 cggccatcgt ggtcggcgcg gttatcgctg ttccggccgc gcggctggtg aagatgaccg
     3361 cgatccctca gatggtcgcg atgttcaacg gcgtcggtgg tgcggcggcc atgctcgtcg
     3421 ctctcgacga gtacgtgcgg ctgcactccg ttgactccac ggcggctgcg atcgcggttg
     3481 ccttcaccgc aatcgtcggc agtgtgagct tcaccggatc gctcgtcacg ttcgccaaac
     3541 ttcaggagtt gatgactggc cggccgatcc tggtgcccgg tgctcaggtc gtgacgcctt
     3601 tggtcggcct cagcgcggtc ggttgcgcaa tcggtgcagt tgcgacgtcc aacgtggcat
     3661 tggttgtcgt ctgcacaggg ctttcactgg cactcggcgt gctattcgtg ctgcccgtgg
     3721 gtggtgccga tgttcccgtc gtgatctcgt tgctgaacgc gttcaccggg ctcaccgtcg
     3781 cggcgtcggg ttacctgctc gacaacacgc tgctgatcgt cgctggcacg ttggtcggcg
     3841 catcaggaac gatgctgacg cagctgatgg cacgcgcgat gggccgcaag atcacggggg
     3901 ttttgttcgg cgcattctct ggtgggtcga gcgccggatc gaccgaagag gtcgcggcca
     3961 ccgaccgcgg cgttcgctct gccggcgctg acgacatcgc cattctcctt gcctacgcgc
     4021 gaaaagtggc catcgtgccg gggtatgggc ttgccgtcgc gcag
//
LOCUS       DASWVF010000648         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_349453, whole genome shotgun sequence.
ACCESSION   DASWVF010000648 DASWVF010000000
VERSION     DASWVF010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349453"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGD55_11445"
     CDS             complement(<1..264)
                     /locus_tag="VGD55_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY0871004.1"
                     /translation="MPSKTFNADAEVPAFAQRVLDLVDAIPAGKVLAYGDIAEYLGEG
                     GPRQVGSVMSTWGGAVPWHRVVHADGTPAPRHDGEVLRLLRADV"
     gene            complement(276..1466)
                     /gene="moeZ"
                     /locus_tag="VGD55_11450"
     CDS             complement(276..1466)
                     /gene="moeZ"
                     /locus_tag="VGD55_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HEY0871005.1"
                     /translation="MESKVSLAPLVEPADSLTVEEVRRYSRHLIIPDVAMAGQKRLKN
                     ARVLAIGAGGLGSPTLMYLAAAGVGTLGVIDYDTVDESNLQRQIIHGQSDIGRSKAQS
                     ARDSIHEINPLVKVVLHEERLDNDNVFEIFNQYDLIVDGTDNFATRYLVNDACVLLGK
                     PYVWGSIYRFDGQASVFWAENGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCASIGSIQ
                     ATEAIKLLTGVGESLIGRLMIYDALEMSYRTVKIRKDPECPICGKNPTITELIDYETF
                     CGAVSDEAVVAAQGSTIVPRELKKWIDDGENIFLVDVREPAEFEIVSIPGATLIPKGE
                     FLNGSALERMPQDKRIVLYCKSGMRSAEALAIVKGAGFADAVHVGSGVVGWVNQVDPS
                     LPSY"
     gene            complement(1480..>2092)
                     /locus_tag="VGD55_11455"
     CDS             complement(1480..>2092)
                     /locus_tag="VGD55_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0871006.1"
                     /translation="EHPELVASLTLVAPALPDLRLRLGSVQLLIALTPLLGPLMVRKL
                     MNADPEWMAKRIYALCYGNPKAISPLRHSRELATLRQRASLPYSAEVYREALRATVMS
                     YIARGPRRMWRQAAAVGVPTLVISGGRDRLVSQRVTARARRTFRDVEMVNLPAAGHVA
                     HLEDPKAVVAAMSAFLSRPDLKRGMSGRRGTVVEGAGRTSPPR"
BASE COUNT          365 a          702 c          677 g          348 t
ORIGIN      
        1 cacgtcagca cgcagcagtc gcaacacttc gccatcgtgg cgcggcgccg gcgtcccgtc
       61 ggcatgcacc acgcgatgcc aaggcaccgc gccaccccag gtcgacatca ccgagcccac
      121 ctgtcgcggg ccgccctcac cgagatattc ggcgatgtcg ccgtacgcca gcactttgcc
      181 tgccggtatc gcgtcgacga ggtcaagcac ccgttgcgcg aaggcgggca cctcggcgtc
      241 cgcgttgaaa gtcttgctcg gcacgaacgg agaagttagt acgacggcag gctcgggtcg
      301 acctgattca cccagccgac gacgccgcta ccgacgtgca ctgcatcggc gaatccggcg
      361 cccttgacga tcgccagtgc ctcggccgag cgcatgcccg acttgcagta aaggacgatc
      421 cgcttgtctt ggggcatccg ctcgagagcc gatccgttga ggaactcgcc cttcgggatc
      481 agcgtcgcgc cggggatcga cacgatctcg aactcggccg gctcacgaac gtcgaccagg
      541 aagatgttct cgccgtcgtc gatccacttc ttcagctcgc gcggcacgat cgtcgagccc
      601 tgagcggcga cgactgcctc atcggagacc gccccacaga acgtctcgta gtcgatcagt
      661 tcggtgatcg tcgggttctt gccacagatc gggcactcgg gatccttgcg gatcttgacc
      721 gtgcggtacg acatctcgag ggcgtcgtag atcatcagcc ggccgatcag cgactcaccg
      781 actcctgtca gcagcttgat cgcctcggtc gcctggatcg agccgatcga cgcgcacagc
      841 actccgagca cgccaccctc ggcgcacgac ggcaccatgc cgggcggcgg cggttctggg
      901 tagaggcacc ggtagcacgg accgttttcg gcccaaaaca cagacgcctg cccgtcgaag
      961 cggtagatcg agccccacac gtacggcttg ccgagcagca cgcacgcgtc gttgaccagg
     1021 tagcgggtcg cgaaattgtc ggtgccgtcg acgatcaggt cgtactgatt gaagatctcg
     1081 aacacgttgt cgttgtcgag ccgctcttcg tgcaacacga ccttgacgag cgggttgatc
     1141 tcgtggatcg agtcgcgggc cgactgcgcc ttcgaccggc cgatatcgct ctggccatgg
     1201 atgatctggc gctgcaggtt cgactcgtca acggtgtcgt agtcgatgac accgagcgtg
     1261 ccgacacctg ctgcggcgag gtacatgagc gtcggtgatc ccagcccacc agcaccgatc
     1321 gccaacacgc gggcgttctt caggcgcttc tgaccggcca tcgcgacgtc gggaatgatc
     1381 aggtgccgcg aatagcgccg gacctcttcg acggtcaggc tgtcggccgg ttcgaccagc
     1441 ggggcaaggg acactttcga ctccatcagc agggcggcat cagcgcggcg gggacgttct
     1501 tccggcgcct tccacaacgg tcccacggcg cccgctcatt ccccgcttca ggtcgggccg
     1561 ggacaggaac gcactcatgg cggccacgac agccttcgga tcctccaggt gggcgacgtg
     1621 gccggctgcc ggcaagttca ccatctcgac gtccctgaac gtacgccgtg ctcgagcagt
     1681 gacccgctgc gagacgagcc ggtcgcggcc gccagaaatg acaagagtcg ggacgccgac
     1741 cgccgcggcc tgtcgccaca tgcggcgtgg tccgcgggca atgtaggaca tcacggtggc
     1801 gcgcaaggcc tcgcggtaga cctcggcact gtaggggagg ctcgcccgct ggcgcagcgt
     1861 cgcgagctcg cgcgagtggc gcagcggcga gatcgctttt gggttgccgt aacacagcgc
     1921 gtagatgcgc ttggccatcc attccgggtc cgcgttcatg agcttgcgca ccatcagtgg
     1981 gccgagcagc ggggtgagcg caatcagcag ttgcacgctg cctagtcgca gccgcagatc
     2041 gggcagcgcg ggggcaacaa gcgtgagcga ggccaccagc tcgggatgtt cg
//
LOCUS       DASWVF010000649         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_43526, whole genome shotgun sequence.
ACCESSION   DASWVF010000649 DASWVF010000000
VERSION     DASWVF010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43526"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGD55_11460"
     CDS             <1..426
                     /locus_tag="VGD55_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0871007.1"
                     /translation="PDLTLCGLTVAVEKGTTEESELPALSTKCTAAGKKAITGLSTDE
                     QNAANLALTSGRADVGSADSPIAAYAVKQLAGKIALAGAIYETAPYGIAIPKKPGLAP
                     AIKAALAAMIADGSYKAVLDKWGDDAGAITTPAINGATS"
     gene            434..1366
                     /locus_tag="VGD55_11465"
     CDS             434..1366
                     /locus_tag="VGD55_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0871008.1"
                     /translation="MTSDSDTAAGRPDTIRAVPVRHPGRWVAIAVIAVLAAMLIHMLL
                     TNPNLEWSAIRKYLFSQIILRGVWMTIKLTVLSMAIGIVLGIVAAVMRLSANPVVRSA
                     AWLYVWFFRGTPLLVQILFWFSISSLFKSLAFGIPFGPHFAGENTNHLISLFSSALLA
                     LSLNEGAYMSEIVRAGILSVDEGQTEAAHALGMTKIKTMRRIVLPQAMRVIVPPTGNE
                     TNSMLKSTSLVSTIGIFDLLRSAQAIYNANYKTIPLLVVVVIWYLAMTSVLQVGQFYL
                     ERHYGRGSTRTMALTPFQRAKLLLSSVQGRGNQL"
     gene            1363..2127
                     /locus_tag="VGD55_11470"
     CDS             1363..2127
                     /locus_tag="VGD55_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0871009.1"
                     /translation="MTQLMVDAQKVHKSFGAVEVLKGIDFQVEPGQVVCLLGPSGSGK
                     STFLRCINHLEKINAGRLFVDGELVGYRQQGDKLHELSEKDVAKKRTEIGMVFQHFNL
                     FPHMTAMGNIIEAPCQVKNEPKAEVRQRATELLAKVGLADKASAYPRQLSGGQQQRVA
                     IARALAMRPKLMLFDEPTSALDPELVGDVLAVMKDLARSGMTMIVVTHEIGFAREVAD
                     TVVFMDGGVVVEQGPPSEVISNPRHERTKTFLAQVL"
     gene            2152..>2696
                     /gene="rfbC"
                     /locus_tag="VGD55_11475"
     CDS             2152..>2696
                     /gene="rfbC"
                     /locus_tag="VGD55_11475"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY0871010.1"
                     /translation="MEVRALAVPGAWEFTPRQIADERGVFLEWFRADIVDRTIGHALE
                     IAQCNQSVSRRGTIRGVHYADVPPSQAKYVYCPGGAVLDVIVDIRAGSPTFGVVDTVR
                     LDNIDRRAVYISEGLGHAFMALTDGATLTYICSSGYAPDREHGVNPLDPALDIPWPKD
                     LEPILSDKDRAAPSLAEAAEQG"
BASE COUNT          506 a          879 c          845 g          466 t
ORIGIN      
        1 cctgacctca cgctctgcgg cctgacggtc gccgtcgaga agggcactac cgaggagagc
       61 gaactgcccg ccctgtcgac caagtgcaca gcggccggca agaaggcgat caccgggttg
      121 tcgaccgacg aacagaacgc cgccaacctc gcgctgacga gtggacgtgc ggatgtcggt
      181 tcggccgact cgccaatcgc ggcatacgcg gtgaagcaac tcgcagggaa gatcgcactc
      241 gctggcgcga tctatgagac cgcgccctac ggcatcgcga ttccgaagaa gcccgggctc
      301 gcgccggcta tcaaggccgc acttgcggcg atgatcgcgg acggcagcta caaggcggtg
      361 cttgacaagt ggggcgacga cgctggagcg atcaccacgc cggccatcaa cggcgccact
      421 agctaacccg tagatgacct ctgacagcga caccgcagcg ggacggcccg acaccattcg
      481 ggccgtcccg gtacggcacc ccggtcgctg ggtcgccatc gccgtcatcg cggttctggc
      541 ggcaatgctg atccacatgt tgctgaccaa ccccaacctt gagtggtcag cgatccgcaa
      601 gtacctcttc agccagatca tcctgcgcgg cgtctggatg accatcaagt tgacggtgct
      661 gtcgatggcg atcggcatcg tgctcggcat cgtggcggca gtgatgcgat tgtccgcgaa
      721 tcccgtcgtc agaagcgccg cctggttgta cgtgtggttc ttccgcggca cgccgctact
      781 cgttcagata cttttctggt tctcgatcag ctcgttgttc aagtcgctgg ccttcggaat
      841 tcccttcggc ccgcactttg cgggcgagaa cacgaaccat ctgatctcgc tcttctcaag
      901 cgcgttgctg gcgttgagct tgaacgaggg cgcgtacatg tcggagatcg tgcgggccgg
      961 aatcctgtcg gtggacgaag gccagaccga ggccgcgcac gcgctcggga tgacgaagat
     1021 caagacaatg cggcgcatcg tgctgccgca agccatgcgc gtcatcgtgc cgccgaccgg
     1081 caacgagacc aacagcatgt tgaagagcac atcgctggtc agcacgatcg gcatcttcga
     1141 tctgctgcgc tccgcccagg cgatctataa cgcgaactac aagacgattc cactgctcgt
     1201 ggtcgtcgtg atctggtacc tcgcgatgac gtcggtgctg caagtggggc agttttactt
     1261 ggagcgtcac tacggtcgcg gctcgacccg cacaatggcg ctcacgccct tccagagagc
     1321 caagctgctc ctgtccagcg tgcaagggcg cgggaatcag ctatgaccca gctcatggtc
     1381 gacgcgcaga aagtgcacaa gtcgttcggc gcggtcgagg tactgaaggg catcgacttc
     1441 caggtcgagc ctggtcaggt ggtctgctta cttggcccgt ctggttcggg caagagcacg
     1501 ttccttcgct gcatcaacca cctcgagaaa atcaacgcgg gccggctgtt cgtcgacggt
     1561 gagttggtcg gctaccggca gcagggcgac aagctgcacg agctctcaga gaaggacgtc
     1621 gccaagaagc ggaccgagat cggcatggtc ttccaacact tcaacctctt tccgcacatg
     1681 accgcgatgg gcaacatcat cgaagcgccc tgccaggtca agaacgagcc gaaggccgag
     1741 gtcaggcagc gggcgaccga actgctggcc aaggtgggcc tcgccgacaa ggcgagtgcc
     1801 tatccccgcc agctctccgg cggacagcag cagcgggttg ccatcgccag ggcgcttgcg
     1861 atgcggccca agctgatgct gttcgacgag ccaacgtccg cgcttgaccc cgaactagtc
     1921 ggcgacgtcc tcgccgtcat gaaggatctc gcccgctcgg gcatgacgat gattgtcgtc
     1981 acccacgaga tcgggttcgc ccgcgaagtc gctgacaccg tagttttcat ggacggcggg
     2041 gtggtggtcg aacagggtcc accatccgag gtcatctcca accctcgcca cgaaaggacc
     2101 aagaccttcc tggcgcaggt tctctgaccc cgcccagtag gctcggcgct catggaagtt
     2161 cgcgcactcg cagtcccagg cgcgtgggag ttcaccccgc gccagattgc tgacgagcgc
     2221 ggggttttcc tggagtggtt tcgcgccgac atcgtcgacc gcacgatcgg gcacgccctt
     2281 gagatcgcgc agtgcaacca gtccgtctcg cggcgcggca cgattcgggg tgtgcactac
     2341 gccgacgtgc ccccgagcca ggccaagtac gtctactgcc caggcggggc ggtgctcgac
     2401 gtgatcgtcg acatcagggc gggctcgccg acattcggag tcgtcgacac cgtgcggctc
     2461 gacaacatcg accgacgggc ggtctacatc tccgagggcc tggggcatgc gttcatggcc
     2521 ctgaccgacg gcgcgaccct gacgtacatc tgctcgagtg gctacgcgcc cgaccgcgag
     2581 cacggcgtga acccgctgga tcccgcactc gacatccctt ggccaaagga cctcgaaccg
     2641 atcctgtccg acaaggatcg cgcggcgccg agcctcgccg aagccgccga acaagg
//
LOCUS       DASWVF010000650         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_170719, whole genome shotgun sequence.
ACCESSION   DASWVF010000650 DASWVF010000000
VERSION     DASWVF010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170719"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1376)
                     /locus_tag="VGD55_11480"
     CDS             complement(18..1376)
                     /locus_tag="VGD55_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0871011.1"
                     /translation="MTTRRRFLRSAAVTVGLPFLPSVMRVGSRARAATCGAPQRFLSW
                     FLPCGVVMPDWTPTTTGTGWTMPYILEPLAPVRSKIVVLTGHDHMVSTTSNPGGHSAG
                     TAGFLTMRKIANDYTDANRTSLDQVIAKQTAVCMRPVGSLQLTLSVPEDSCDSAPCQF
                     VHTVSYDANNPLPHQVSPLQAFNSVFTGTSTTASIADAARRQALRQSVLDHVLGETQS
                     LRTSLSPSDRLKLDEYQTSVRTLEMQIRNMASSAQCAMAKSPGMDAVPYADQVKAMID
                     IIALAFQCDMTRVVSFMMARGSSLQDWSFLVNQSSPHHRVSHHNNDANNLAMLRQINR
                     WEITQLAGLLQRLDAMQDVNGKTVLDNTLMYCSSEISDGNSHNKWDMPVLLAGSAGGA
                     LKIDGRHISYTKMTFPRPIVGPQGGPHTEHTFVSILNAFGIAANTFGDATVSGPNTDI
                     LP"
     gene            complement(1373..>1667)
                     /locus_tag="VGD55_11485"
     CDS             complement(1373..>1667)
                     /locus_tag="VGD55_11485"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HEY0871012.1"
                     /translation="DNGSPIDATGSIGNTKFDGAVGLANAVASSPQFVPCMAQQALTY
                     AVGRSFEGADARAYAAGLGTKLVADGHGTWRGLFAAVATSEAFTTRRGEATTP"
BASE COUNT          248 a          542 c          576 g          301 t
ORIGIN      
        1 tccgcgcatg gtgcgtttca cggcaagatg tccgtgttgg gcccgctgac cgtcgcgtcg
       61 ccgaaggtgt tcgccgcaat cccgaacgcg ttcaggatcg acacgaacgt gtgctccgtg
      121 tgcgggcccc cctgcggccc cacaatcggg cgcgggaacg tcattttcgt gtagctgatg
      181 tgccgcccgt cgatcttcag ggcgcccccg gcgctgcccg ccagcagcac gggcatgtcc
      241 cacttgttgt ggctgttccc gtcggagatc tcgctgctgc aatacatcag cgtgttgtcg
      301 aggaccgtct tgccgttgac gtcctgcatg gcgtccagtc gctggagcag cccagccagc
      361 tgcgtgatct cccagcggtt gatctgccgg agcatcgcca ggttgttcgc gtcgttgttg
      421 tgatgtgaca cgcgatgatg cggcgacgac tgattcacca ggaacgacca gtcctgcagg
      481 ctggatccgc gcgccatcat gaagctcacg acgcgcgtca tgtcgcactg gaacgctagc
      541 gcgatgatgt cgatcatggc cttcacctgg tccgcgtacg gaacggcgtc catgcccggc
      601 gacttcgcca tcgcgcactg cgcggacgac gccatgttgc gaatctgcat ctccagcgtg
      661 cggacggagg tctggtactc gtcgagcttg aggcgatcgg acgggctcag cgacgtccgc
      721 aggctctgcg tttcgccaag gacgtgatcg agaacgctct ggcgcaacgc ttgccggcgg
      781 gcggcatccg cgatgctggc cgtggtgctg gttcccgtga agacgctgtt gaacgcttgc
      841 aacggcgaca cttggtgcgg caacgggttg ttcgcgtcgt acgaaaccgt gtgcacgaac
      901 tggcaggggg cggagtcgca gctgtcctcg ggcaccgaca acgtgagctg cagcgagccc
      961 accgggcgca tgcagacggc cgtctgcttg gcgatgacct ggtccaggct ggtgcggttt
     1021 gcgtcggtgt agtcgttggc gatctttctc atcgtcagaa agcccgcggt ccccgccgaa
     1081 tggccacccg ggttgctggt ggtcgacacc atgtggtcgt gccccgtcag cacgacgatc
     1141 ttcgagcgca cgggggcgag cggctccagg atgtacggca tcgtccaccc ggtgccggtg
     1201 gtcgtcggcg tccagtcggg catcacgacc ccgcacggca ggaaccacga caagaagcgc
     1261 tgcggggcgc cgcaggtggc ggcccgcgcg cgcgaaccga ccctcatgac ggacggcagg
     1321 aacggcaggc cgactgtgac ggccgcgctt cgcaagaatc gtctccgcgt tgtcatgggg
     1381 tggtcgcctc tccgcgccgg gtggtgaacg cctcgctggt cgccacggcg gcgaagaggc
     1441 cgcgccaggt gccgtgcccg tccgcgacca gcttggtccc caagccggcg gcgtacgcgc
     1501 gcgcgtcggc gccctcgaac gagcgcccca cggcgtaggt cagcgcctgc tgcgccatgc
     1561 atggaacgaa ctgagggctg ctggcgacgg cgttggccag tccgacggcg ccgtcgaact
     1621 tcgtgttccc gatcgagccg gtggcgtcga tcggggagcc gttgtcg
//
LOCUS       DASWVF010000651         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_374963, whole genome shotgun sequence.
ACCESSION   DASWVF010000651 DASWVF010000000
VERSION     DASWVF010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374963"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGD55_11490"
     CDS             <1..188
                     /locus_tag="VGD55_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871013.1"
                     /translation="LVWKRINCHAKGCYRVGLHHVDGTPYITCGKHHPTHPGSGPATA
                     EEIADAHAMAHASTPAA"
     gene            214..1062
                     /locus_tag="VGD55_11495"
     CDS             214..1062
                     /locus_tag="VGD55_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0871014.1"
                     /translation="MPNVALSWQAATLIGAACIAAAAIVGRVASDDGRWWLKVAPFGR
                     EVGIILGLYALWQIAGGVSLGHTDEAVRRGAWLWQHERGWRFPSEVAVQRPILGHPLI
                     VQFCNIYYATMHFGMLLAVLLWLYIRHRDAYVRTRTLIVLTTTACLLVGLVPVAPPRL
                     VHVGMVDTAALYGQSVYAVTGRLGADQYSAMPSVHVAWAFLVAVAVIMATRSRWRWLI
                     LVHTALTLYVVVATANHFWLDGVVAVALLAISLVVQVVSRRLSLRWRRPIIEQVDAED
                     LVASRS"
     gene            complement(1067..1540)
                     /locus_tag="VGD55_11500"
     CDS             complement(1067..1540)
                     /locus_tag="VGD55_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0871015.1"
                     /translation="MLSLTYWGFDGQPHQGALVVNASVVPAVTQAFSTLFAEHFPIRT
                     MIPIAAFGASDPASTAADNTSSFNCRYAVANGPPQWSAHAYGEAIDINTVENPYVFNG
                     QVLPANATAYRVRSPYRPGMAVPGGQLVEAFAAVGWQWGGRWTAPDYQHFSSTGG"
     gene            1539..1838
                     /locus_tag="VGD55_11505"
     CDS             1539..1838
                     /locus_tag="VGD55_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871016.1"
                     /translation="MRNCAGPTGHPACQLFASVAAVTAVALATKTSGTTGAGAGGLGT
                     VVGTEVGVEVGVEVGADGRGFAGCVGVGARVLRLGVAMAGLEVGVGGVTGVDAAG"
     gene            1848..2813
                     /locus_tag="VGD55_11510"
     CDS             1848..2813
                     /locus_tag="VGD55_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY0871017.1"
                     /translation="MLAIAVLAGVPANPAAELTAAVLTAAVLTAAAELPPACGCPLVH
                     PATRTRQARAAIDAHRLGVIRVRLARRILRRRRKCDRIDAVNHEHDLVDTIEMYLRTI
                     FELEEEGAVPLRARIAERLMQSVPTVSQTVARMERDGLVTVEGDRRLELTPAGRSLAT
                     RVMRKHRLAECLLVDVIGLEWEFVHEEACRWEHVMSEAVERRLVDVLGHPQESPYGNP
                     IPGLAELGEGTGDVEFREGLVALSTVTDMTQSVIVRRIGEPLQSDTALMSRLRRAGIR
                     PNLPVRISRVDGRLLVGRDGDAAEIDDVVASHVFVSTAPVVATAS"
     gene            complement(2823..3341)
                     /locus_tag="VGD55_11515"
     CDS             complement(2823..3341)
                     /locus_tag="VGD55_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871018.1"
                     /translation="MQPIVTRKTPAAADAREQRDVRMMGLPVALFLRTQEHHDELVRE
                     FTLMAIRESATDVGSTPRTVLPARLRELIDILGRRFDASTSRADIELQAAVERGDPTV
                     DLTYHVRSHVAAELIVSTELMDDADEFCRQEKLLTLPRDAAMVAFSRWYNREFLRQLD
                     GLPPTPWDGPVS"
     gene            complement(3446..3616)
                     /locus_tag="VGD55_11520"
     CDS             complement(3446..3616)
                     /locus_tag="VGD55_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0871019.1"
                     /translation="MSATDKVKNAAQSAKGDIKKAAGHATNNPRLEAEGQADKTGGSL
                     KQAGEKVKDAFK"
     gene            3896..>4339
                     /locus_tag="VGD55_11525"
     CDS             3896..>4339
                     /locus_tag="VGD55_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0871020.1"
                     /translation="MSLVSADAHADHENFEDPGEYEPETAAEPAPGTGAHGSPETGAK
                     GAVAAHPGNREVLIAKIVVLDTSALMSDPEGIFDAFPGADMVIPLTVVQELDGLKKRL
                     DPAGGAARDVLRRIEQLRLAAGGDLRKPLERPFGGPVRMQVNGVSK"
BASE COUNT          665 a         1409 c         1480 g          785 t
ORIGIN      
        1 gcctggtgtg gaagaggatc aattgccacg ccaagggctg ctatcgggta ggcctgcatc
       61 acgtcgacgg caccccctac atcacctgcg gcaagcacca cccaacgcac cccggcagcg
      121 gacccgcgac cgctgaagag attgcggacg cccatgcgat ggcgcacgca tccacccctg
      181 ccgcctgacc gtggccatgc gcgatgatgt ctcgtgccca acgtcgccct gagctggcaa
      241 gccgccactc tgatcggtgc tgcgtgcatc gcggccgcgg cgatcgtggg tcgcgttgcc
      301 tcggatgatg ggcgctggtg gctgaaagtc gcgccgttcg gccgcgaggt cggcatcatc
      361 ctcggcctgt atgcgctgtg gcagatcgcg ggcggcgtct cgctcggtca caccgacgaa
      421 gcggtgaggc gcggcgcctg gctttggcaa cacgagcgtg ggtggcgttt cccgagtgag
      481 gtcgcggttc agcgcccgat cctcggccac ccactgatcg tgcagttctg caatatctat
      541 tacgcgacga tgcatttcgg catgctgctc gccgtcctgc tgtggcttta catccgtcat
      601 cgcgatgcct acgtgcgcac ccgcactctc atcgtgttga cgacgaccgc gtgcctgctg
      661 gtcggccttg tgcccgtcgc gccgccgcga ctcgtccacg tcggcatggt cgacaccgcc
      721 gctctgtacg ggcagtcggt gtacgcggtc accggccggc tcggcgcgga tcaatactcc
      781 gcgatgccgt cggtgcacgt ggcgtgggca tttctggtcg cggtcgcggt cattatggcg
      841 acgcgcagtc gctggcgctg gttgatcctc gtgcacaccg cgctgacctt gtacgtcgtc
      901 gtcgcgaccg cgaatcattt ctggctcgac ggagtggtgg ccgtcgcgtt gctcgcgatt
      961 tcccttgtgg tgcaggtggt ttcgcggcgg ctctccctcc gttggcggcg cccgatcatc
     1021 gagcaggtgg acgccgagga cctcgtcgcg tctcgcagct aaccgcttac ccgccggtcg
     1081 acgagaaatg ctgatagtcg ggtgcggtcc aacggccacc ccattgccag ccgactgctg
     1141 cgaacgcctc gacaagctgg ccgcccggta ctgccatgcc aggccgatac ggcgagcgca
     1201 cccgataggc ggtggcgttc gctggaagaa cctggccgtt gaagacataa ggattctcga
     1261 ccgtgttgat gtcgatcgct tcgccatagg cgtgcgccga ccattgcggc ggcccgttcg
     1321 ccactgcgta ccggcagttg aagctcgacg tgttgtcggc ggcggtcgac gctggatcgc
     1381 tcgcgccgaa tgcagcgatc gggatcatcg tgcggatcgg gaaatgctcg gcaaacaagg
     1441 tgctgaacgc ttgcgtgacg gccggcacga cggatgcgtt cacgacgagc gcaccctggt
     1501 gcggctgccc gtcgaatccc cagtaggtca agctcagcat gcgcaactgc gcgggcccga
     1561 ccgggcaccc cgcgtgccaa ctcttcgcga gcgttgccgc cgtgacggcc gtggcgttgg
     1621 cgacgaagac atcagggacg acgggtgcgg gcgccggcgg gctcggcacc gtggtcggca
     1681 ctgaggtcgg cgttgaggtc ggcgttgagg tcggcgctga cggccgcggt ttcgccggat
     1741 gcgtcggtgt cggggcgagg gtcttaaggc tcggggtggc gatggccggg cttgaggttg
     1801 gggtgggcgg cgtgacgggg gttgatgcgg ctggctgact caccgccgtg ctcgcgatcg
     1861 ccgtgctcgc gggcgtccct gccaacccgg ccgccgaact caccgccgcc gtactcaccg
     1921 ccgccgtact caccgccgcg gctgagctgc cgcccgcgtg cggatgcccg ctcgtgcacc
     1981 cggcgaccag gaccaggcag gcgagggcgg ctatcgacgc gcaccgactg ggagtcatca
     2041 gggtcaggct agcccgccga attctgcgca ggcgccgcaa gtgcgacaga atcgatgccg
     2101 tgaaccacga gcatgatctt gtcgacacga tcgagatgta cctgcgcacg atcttcgagc
     2161 tcgaagaaga aggcgcggtg cccttgcgtg cacggatcgc cgagcggctc atgcagagcg
     2221 tcccgacggt gtcgcagacc gtggcgcgca tggagcgcga tgggctggtc actgtcgagg
     2281 gcgatcgtcg gctcgagctc accccggccg gccggtcgct ggcaacgcgg gtcatgcgca
     2341 agcatcgcct cgccgagtgc ctgctcgtcg acgtgatcgg cctcgaatgg gagttcgttc
     2401 acgaagaggc ttgtcgttgg gagcacgtca tgagcgaggc ggtagagcgc cggctcgtcg
     2461 acgtcctcgg gcatccgcag gagtcgccat acggaaaccc gatcccgggc ctggccgagc
     2521 tcggcgaagg caccggtgac gtcgagttcc gcgaaggcct cgtcgcgctg agcaccgtca
     2581 ccgacatgac gcagagcgtc atcgtgcggc gcatcggcga gccgctgcaa tccgacaccg
     2641 cgttgatgtc gcggctgcgg cgagccggca tcagacccaa cttgccggtg cgcatctcgc
     2701 gagtcgacgg ccgcctgctc gtcggccgcg acggggacgc tgccgaaatc gacgacgtgg
     2761 tggcatcgca cgtcttcgtg tcgactgcac cggtggtcgc caccgcgtcg tgatcggtgc
     2821 cgctagctga ccgggccgtc ccatggcgtt ggcggcaggc cgtcgagttg gcgaagaaac
     2881 tcgcggttgt accaacgact gaacgcgacc attgcagcgt cgcgtggcaa cgtcagcagc
     2941 ttctcctgac ggcagaattc gtcggcgtca tccatgagtt cggtactcac gatgagctcg
     3001 gcggcgacat gacttcgcac gtggtaggtc aagtcgaccg tcggatcgcc acgttcgaca
     3061 gcggcctgaa gctcgatgtc ggctcgtgac gtgctcgcgt cgaatcggcg tccgagaatg
     3121 tcaatcaact cgcgaagccg ggccggcaaa acggtgcgcg gcgtcgatcc gacatcggtt
     3181 gcgctctccc ggatcgccat cagcgtgaac tctcggacga gttcatcgtg gtgctcttgc
     3241 gtgcgcaaga agagggctac gggcaaaccc atcatgcgga cgtcgcgttg ctcgcgtgca
     3301 tcggccgccg cgggtgtttt tcgagtgacg atcggttgca cgcaggcatt ttcgctgatg
     3361 agagccgcgg attcgcgcag ctagaccgcg acatgcagga gggccggccc gcaccgaccc
     3421 tcccggcgtt tatttcagtg cacgactact tgaacgcgtc cttgactttc tcgccggcct
     3481 gcttcaacga tccgcccgtt ttgtcggctt gtccttctgc ctcgagtcgc gggttgttgg
     3541 tcgcgtgtcc ggcagccttc ttgatgtcgc ccttagccga ctgtgcggcg ttcttgacct
     3601 tgtcagtggc gctcatgcgg tcaacctcct catggtcttg acggtcttgc gtgaagcgga
     3661 gcttgcacgc gtgagagcac ctacccgtcc cgccggggtc tatgcaccgg ccccacggct
     3721 taaaaaacgg ccgtccgcgg cgagcgtcgc gacacgatct tgagcgcgcg ccgcgcctcc
     3781 ccccaacgtc gcgactcgcg cgacaccatc ggcgaagcga ctgcccgggc gcattacggt
     3841 ggtcgtgccc aggcaccgct ggtagcggcg gttgccgtga cgacgggatg gctgcatgag
     3901 cctcgtttca gccgacgcgc acgccgatca cgagaacttc gaagatcccg gggagtacga
     3961 gcctgaaacg gccgccgagc ctgcgcctgg caccggcgcc cacggctctc ccgaaacggg
     4021 cgcgaagggc gcggtcgcgg cgcatcctgg taacagggag gtcctcatcg ccaagatcgt
     4081 ggtgctcgac acgtcggccc tgatgtcaga tccggaaggg attttcgatg cgtttccggg
     4141 cgccgacatg gtcattccgc tcaccgtggt gcaagaactc gacggcctga agaagcggct
     4201 cgatcccgca ggtggggcag cacgcgacgt cctgcgtcgg attgagcagt tgcgactagc
     4261 cgcgggtggc gacctgcgta agccgttgga gcggccgttc ggtggcccgg tgcgcatgca
     4321 ggttaacggt gtgtcgaag
//
LOCUS       DASWVF010000652         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_502622, whole genome shotgun sequence.
ACCESSION   DASWVF010000652 DASWVF010000000
VERSION     DASWVF010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502622"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1541)
                     /locus_tag="VGD55_11530"
     CDS             complement(300..1541)
                     /locus_tag="VGD55_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY0871021.1"
                     /translation="MSPEQAAAAAMVAEARERGLELTGPNGLLKLFTKNVLETALNEE
                     ITEHLGHAKNRADPGRETTNVRNGSRSKTVISDAAGEVDIDVPRDRAGTFEPQIVRKR
                     QRRLADVDEIVLSLYAKGLTTGEISAHFAEIYGASVSKETVSRITESVMAEMQDWAGR
                     PLDEVYVAVFVDAIMVKVRDGQVANRPMYAAIGVTVDGRKDVLGLWAGSGGEGAKFWL
                     SVLTDLRNRGVRDVFFLVCDGLKGLPEVVENVWPAAIVQTCIVHLIRNTFRLTSKRDT
                     EAIKRDIRRIYTAPTPDAALAALDDLEEKWGKTYRAMIRMWRNAWTEFVPFLDYDTEI
                     RTVLCSTNAIESLNARYRRAVRARGHFPTENAAMKCLYLVTRSLDPTGTGRARWTVRW
                     KPVINAFAITFSDRWPSAESY"
     gene            1787..>2035
                     /locus_tag="VGD55_11535"
     CDS             1787..>2035
                     /locus_tag="VGD55_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871022.1"
                     /translation="MARQAADRRGERERRQQERDAVLRELAAQRQLVATIAQRTERAE
                     QARELRAQQEDTAARLDADAGAELEQLTGLLAAAAAEPA"
BASE COUNT          368 a          685 c          616 g          366 t
ORIGIN      
        1 accagctgca cgatcctcgt cccgagcccg gtgagcacga cacgcgaccg ccaagccctc
       61 gaatcccaac aagcacgacg catcatcgct accgtcgacc acggtccgcg gctctccctt
      121 cgctgctcca agacacagcg aggatcaagg agtcgcgggc cgcccgcgtt caggcgcgcc
      181 gcacgggccc aacctcaggt tgcccgccaa gattgaagag ccctttaact caatcttcac
      241 gaaccatcga cacgctgtgg gcgggtctgt aactggaacg gtggttccgg cgttgttggt
      301 cagtaactct cagcggacgg ccagcggtca ctgaaggtga tcgcgaacgc gttgatcacc
      361 ggtttccacc ggaccgtcca tcgtgcgcgg cctgtcccgg tcgggtccag gctgcgggtg
      421 acaaggtaca ggcacttcat cgcggcgttc tcggttggga aatgaccacg agcacgtact
      481 gcccgccggt accgagcgtt caaggattcg atcgcgttgg tcgagcacaa cacggtacgg
      541 atttcggtgt cgtagtcgag gaacggtacg aactcggtcc aggcgttgcg ccacattcgg
      601 atcatcgccc gatacgtttt tccccatttc tcttccagat cgtccagggc ggcgagcgcg
      661 gcgtcgggag tgggcgcggt gtagatacgc cgaatatccc gcttgatcgc ctcggtgtcg
      721 cgtttcgagg tgagccggaa cgtgttgcgg atcagatgca cgatgcaggt ctgcacgatc
      781 gccgcgggcc acacgttctc cacaacttcc ggtagtcctt tcaggccgtc gcagacgagg
      841 aagaacacat cacgcacgcc gcggttgcgt aggtcggtca gcacgctgag ccagaacttc
      901 gcaccctccc caccactgcc cgcccacagt cccagcacgt ccttgcggcc gtccaccgtg
      961 acgccgatgg cggcgtacat gggccggttg gcaacctggc cgtcgcggac cttgaccatg
     1021 atcgcgtcca cgaacaccgc gacgtatacc tcatcaagcg ggcgacccgc ccagtcctgc
     1081 atttccgcca tcacggactc ggtgatccgc gaaactgttt ccttcgacac tgacgcccca
     1141 taaatctcgg cgaaatgcgc cgagatctcc ccggtcgtca aacccttcgc atacaacgac
     1201 aacacgatct cgtccacatc cgcgaggcgt cgctgtcgtt tgcgcacgat ctgcggctcg
     1261 aacgtgccag cacggtctcg tggcacgtcg atgtccactt ctccggccgc atcggagatc
     1321 accgtcttcg accgtgaacc atttcgcaca ttcgtcgtct cgcgaccggg atcggcgcgg
     1381 ttcttcgcat gcccgagatg ctcggtaatc tcctcgttca gcgcggtttc caacacgttc
     1441 ttggtgaaca gtttcaacag cccgttcggg ccagtcagct cgaggccacg ttcccgggcc
     1501 tcggccacca tcgccgccgc ggcggcctgt tccggcgaca gctgtcgccg ggcttgcctc
     1561 tcatccttgc gtggactcac agggtccgat gtcatcactc acagtgccca tcccgtcgga
     1621 tctcagcccg gcgtgtcggg gccgaaacca ccgttcgctg cacaggcccc atagagcggc
     1681 cgaacccgac acgacaagat tcgtcaccgg ggcagcggtg ccaccggcca cacgcttcgt
     1741 gatgaggtta ttgcggggtg atgaggcggt gtcgatttgg gatgagatgg ccaggcaggc
     1801 ggctgaccgt agaggggagc gtgaacggcg gcagcaggaa cgcgacgctg tcctccgcga
     1861 gcttgccgct cagcggcagc tggtcgcgac catcgcgcaa cgcactgaac gtgcggagca
     1921 agcacgggaa ctgagggcgc agcaggagga caccgctgcc cggttggatg ccgacgccgg
     1981 ggcggagtta gagcagctca ccggcctgct ggcagcggca gccgctgaac ccgcg
//
LOCUS       DASWVF010000653         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_120236, whole genome shotgun sequence.
ACCESSION   DASWVF010000653 DASWVF010000000
VERSION     DASWVF010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120236"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="VGD55_11540"
     CDS             <1..1769
                     /locus_tag="VGD55_11540"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0871023.1"
                     /translation="LERAATRIGRTLTAEAATTPPSAELSAAIDEARRDHPELIGGLA
                     LRAPDEPHRLFVLYTAARLEARRLGEPGGYRRPDELIEQLVLVQESLAAAGAHRLAFG
                     ELQHLIWQARTFGFHLAELEVRQHSGVHARALQEIGAGGDVSGAVSAMTDEVLETIRA
                     VAWLQGEYGVAACRRYVISFTTSAADIAAVFELASIATAGEPPVLDVVPLFETQDDLQ
                     RCVVVLEEALQLPPVQARLAANGRRLEVMLGYSDSAKDVGPVSATLALYDAQGALAAW
                     AKQHGITLTLFHGRGGALGRGGGPANRAVLSQAPGSVDGRFKVTEQGEVIFARYGHPV
                     IAHRHLDQVAAAVVLASTPAVEELVAAAATKHRATFEQVSDAARTAYRELVGADRFAE
                     FFAAVSPLAEIGSLRIGSRPSRRTAGSGLSDLRAIPWVFAWSQTRATLPGWYGLGSGL
                     EVVGDVSVLQAAYAQWPLFTTMLDNVEMSLAKADRDIAQRHLALGGRPDLADTILAEF
                     DRTTSWLLRVLGQEKLLEKHSVLGRAVELRNPYVDALSHLQLRALEALRAGIDDDDER
                     ERTQRLLLLAVNGVAAGLQNTG"
BASE COUNT          289 a          626 c          656 g          329 t
ORIGIN      
        1 cactcgaacg tgccgccact cgtatcggtc gcacactgac ggccgaagca gccaccaccc
       61 caccaagcgc cgagctgagt gcggcgatcg acgaagctcg gcgcgaccat cccgagctga
      121 tcgggggcct cgcattgcgt gctcccgacg aaccgcaccg gctcttcgtg ctctacacag
      181 cggctcgtct tgaggcgcgg cggctcggcg agcccggcgg ctaccggcgt cccgatgaat
      241 tgattgaaca gctcgtactc gtgcaggagt cgcttgccgc ggcaggcgct catcggttgg
      301 cgttcggtga gttgcagcac ctgatctggc aggcgcgcac atttgggttc cacctcgctg
      361 aactcgaggt tcgccagcac agcggcgtcc acgctcgcgc gttacaggag atcggcgcgg
      421 gcggcgatgt gtcgggcgct gtgtcggcga tgaccgacga ggtgctcgag acgattcgcg
      481 cggtggcgtg gctgcaaggc gagtacgggg ttgccgcgtg tcgccgctac gtcatcagct
      541 tcaccacatc ggcggctgac atcgcggcgg tgttcgagct tgcgtcgatc gcgacggccg
      601 gcgaacctcc ggtgctcgat gtcgtcccgc ttttcgagac gcaggacgac cttcagcgat
      661 gcgtggtcgt gctcgaggag gcgctgcaac tgccgcccgt gcaggcccgg ctggcggcta
      721 acggtcgccg gctcgaggtg atgctcggct actccgactc tgcgaaggac gtggggcccg
      781 tctccgcgac gcttgcccta tacgacgcgc agggggcatt ggccgcgtgg gcgaagcagc
      841 acggcatcac gttgacgttg ttccacgggc gcggcggtgc cctgggtcgg ggcggggggc
      901 cggcgaaccg tgcggtgctc agccaagctc ccggatcggt cgacggccgc ttcaaagtga
      961 ccgagcaggg cgaggtcatc ttcgcgcgct acgggcatcc ggtgattgcg caccggcatc
     1021 ttgaccaggt cgcggcggcg gtcgtgctcg catcgacgcc cgcggtcgaa gaactcgtcg
     1081 cagctgccgc aaccaagcat cgagcgacgt tcgagcaggt cagcgacgcg gcccgcacgg
     1141 cttaccgcga gctagtcggt gccgaccggt tcgctgagtt cttcgcggcc gtcagcccgc
     1201 tcgctgagat cggttcgctg cgcatcgggt cgcggccctc tcgccgtact gcggggagcg
     1261 gactttctga cctgcgcgcg atcccctggg tgttcgcgtg gtctcagacc cgcgcaacgc
     1321 tccccggttg gtacggcttg ggcagcggac tcgaagtggt cggcgacgta tccgtgctcc
     1381 aggccgcgta tgcgcagtgg ccgctgttca cgacgatgct cgacaacgtc gagatgtcgc
     1441 tggccaaggc cgaccgcgac atcgcccaac gccatcttgc gttaggtggc cgcccagact
     1501 tggccgacac gatcttggcc gagttcgacc gcactacgtc atggctgttg cgagtgctcg
     1561 gccaggagaa acttctcgag aagcacagcg tgctcggccg cgctgtcgag ctgcgaaacc
     1621 cttacgtaga cgcactttcg catctccaac tgcgtgccct cgaggcgttg cgcgcaggca
     1681 tcgacgacga cgacgaacgt gagcgcacgc agcggttgct gctgctcgcg gtaaacgggg
     1741 ttgcggccgg gctgcagaac accggctgag ctgtgacgtc gccctggacc gacctcgaac
     1801 ggccgccgct atcggtgcga gcgctgactc gcggcctcgt cgactacggc atctggcgtg
     1861 agatcagcgt cgtcgaagag accggttcga ccaacgcgga
//
LOCUS       DASWVF010000654         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_451912, whole genome shotgun sequence.
ACCESSION   DASWVF010000654 DASWVF010000000
VERSION     DASWVF010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451912"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGD55_11545"
     CDS             complement(<1..268)
                     /locus_tag="VGD55_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0871024.1"
                     /translation="MTLGVRESGTADGLPVLHFHGTPGSRLELAWADDAVAQAGVQLV
                     TFDRPGYGRSTQVPFSLSRVAHLAMELADRLGWQRFATLGWSGGG"
     gene            complement(273..524)
                     /locus_tag="VGD55_11550"
     CDS             complement(273..524)
                     /locus_tag="VGD55_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871025.1"
                     /translation="MVISKRVAWFLIAFGVWSWVIWPVFLKNIWKDSRSWNHGMTAFF
                     AVHLVLTVVSLVLGTAIGVLGIRAVRILSIRGQRLHGHH"
     gene            596..940
                     /locus_tag="VGD55_11555"
     CDS             596..940
                     /locus_tag="VGD55_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0871026.1"
                     /translation="MVVLQSSDAEVDRVFQALADATRRDILTRARDGQSVSALARHYD
                     MSFAAVQKHVAVLERAALVTKRRRGREQIVQGNEATLRAATELLAAFEQIWIGRATQI
                     AGILADEDGEIA"
     gene            937..1443
                     /locus_tag="VGD55_11560"
     CDS             937..1443
                     /locus_tag="VGD55_11560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0871027.1"
                     /translation="MTVITSTKDPVAMTMTVTAEFDASPDRVWTVWEDARKLERWWGP
                     PTYPATFTRHDFVVGGESRYFMTGPAGETPGGWWRIDALDSPHRIEFANGLAGEDGEP
                     KPGVEPMPSNVTFEAVDGGTRMTVTTRFVSVEQMQTLLGMGMQEGMTQAVGQIDALLS
                     PAAAVSHP"
     gene            complement(1456..>2805)
                     /locus_tag="VGD55_11565"
     CDS             complement(1456..>2805)
                     /locus_tag="VGD55_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0871028.1"
                     /translation="HPHAIAVRRATARLYKTVKLRNRKEKRSSVVAADEAITAATATG
                     APGRIDDETRGLALTASATAPIVGVLGKARACYICKERYRDVDAFYHQLCPKCAALNR
                     SRRDARADLTDRRALLTGGRAKIGMYIALRLLRDGAHTTITTRFPNDAVRRFAAMHDS
                     SQWLHRLSIVGIDLRDPSQVVALADAVAAAGPLDILINNAAQTVRRTPGAYAQLVAAE
                     SAPPSEGPLPELLDLGYSVGTAPAAIGGHFPTHALTPHALTALALTSGSASLDRVAEQ
                     TAIDAGGLVPDLDAVNSWTRRVHEVDPVELLEVQLCNMIAPFILISRLRPSLAAATAR
                     RKYVVNVSAMEGQFGRIYKGAGHPHTNMAKAAVNMLTRTSARDLFAERILMTSVDTGW
                     ITDERPHPTKARLAGEGFHAPLDLIDGAARVYDPIVRGEQGEDLYDCFLKDYAKSAW"
BASE COUNT          547 a          907 c          893 g          458 t
ORIGIN      
        1 gcccgccgcc cgaccaaccg agcgtcgcga accgttgcca cccgagacgg tcggcaagct
       61 ccatggcgag gtgcgcgacc cgcgatagcg agaacggcac ctgcgtggat cgcccgtagc
      121 caggtcggtc gaacgtgacc agttgaacgc ccgcttgcgc caccgcatcg tcggcccatg
      181 ccaactccaa gcgcgacccc ggcgtcccgt gaaaatgcag tacgggcagc ccatcagcag
      241 tgccggactc gcgcacgcca agggtcacgt catcaatggt gaccgtgtag tcgctgacca
      301 cgaatagaga gtatgcgcac tgcgcggatt ccgagcaccc cgatcgccgt cccgagaacg
      361 agcgagacga cggtcaacac gaggtggacg gcgaagaacg ccgtcatccc gtgattccac
      421 gaccgcgagt ccttccagat gttcttcagg aagacgggcc agatcaccca actccacaca
      481 ccgaaggcga tcaagaacca cgcgacccgc ttggaaatga ccatggtcga agtatcccgg
      541 ctcgcacacc gctgccactt gcatcgaacg caagagtcct ctaacgtaca accaaatggt
      601 tgtacttcag tcgagcgacg ccgaggtcga ccgcgtcttc caggccctgg cggacgcgac
      661 ccggcgcgac atccttacgc gcgcgaggga cgggcagtcg gtatcggctc tcgcccggca
      721 ctacgacatg agcttcgccg cagtgcaaaa gcacgtcgcg gtattggaac gggccgcgct
      781 cgtcaccaaa cgaagacgag gacgcgagca gatcgtgcag ggaaacgagg caactcttcg
      841 agccgcaact gaactgctcg cagccttcga gcagatatgg atcgggcggg ccactcaaat
      901 cgccggcatc ctcgccgacg aggacggaga aatcgcatga ctgtcatcac cagcacgaag
      961 gacccggtcg cgatgaccat gaccgtgacc gcagaattcg acgccagccc ggatcgggtc
     1021 tggaccgtgt gggaagacgc ccggaagctg gagcgctggt ggggaccgcc gacctacccc
     1081 gcgacgttca cgcgacacga cttcgtcgtc ggaggcgagt cgcgctactt catgacaggc
     1141 ccggctggcg agacgccggg tggctggtgg cgcatcgacg cgctcgacag tccgcaccgc
     1201 atcgagttcg ccaacggcct cgcgggcgag gacggcgaac cgaagccagg agtcgagccc
     1261 atgccgagca acgtcacctt cgaagctgtc gacggcggca cccggatgac ggtcacgacc
     1321 cgcttcgtca gcgttgaaca gatgcagacg ctgctcggca tgggcatgca agaaggcatg
     1381 acccaggcag tcggtcagat cgatgcgctg ctgtcccccg cagcagccgt cagtcaccct
     1441 tgacgtaccg ggtgactacc aggcggactt cgcatagtcc ttgaggaagc agtcgtacag
     1501 gtcttcgccc tgctccccgc gcacgatcgg gtcgtagacg cgagccgcgc cgtcgatgag
     1561 atcgagcggg gcatggaacc cctcgccggc aagacgggcc ttcgtcggat gcggccgttc
     1621 gtcggtgatc cagccggtgt cgacactggt catcaagatg cgctcggcga acaggtcgcg
     1681 cgcgctcgtg cgcgtcagca tgttcaccgc ggccttcgcc atgttggtgt gcggatgtcc
     1741 ggcgcccttg tagatgcggc cgaactgccc ttccatcgct gacacgttca cgacgtactt
     1801 gcgtcgagcc gtagcggccg ccagcgaagg ccggagccgg cttatgagga tgaacggcgc
     1861 gatcatgttg cacagctgca cctcgagcag ctcgactggg tcgacctcgt gcacccgcct
     1921 ggtccagctg ttcacggcgt cgagatcagg gaccagcccg ccggcgtcga tcgccgtttg
     1981 ttcggcaaca cgatcgaggg acgccgatcc gctggtcagt gccaacgctg tcagcgcatg
     2041 tggtgtcaat gcgtgggtag ggaaatggcc gccgatcgcg gcaggtgcag tgcctacgga
     2101 ataaccgaga tcgagcagtt cgggtaacgg cccttcagat ggtggcgcac tttcggctgc
     2161 gaccaattgc gcgtaggcac cgggcgttcg acgcacggtc tgcgccgcat tgttaatgag
     2221 aatgtcgaga ggcccagctg ctgcaactgc atcggccaac gcgaccacct gcgaggggtc
     2281 gcgtaaatcg atacccacaa tgctcagtct gtgcaaccac tgagagctgt cgtgcatcgc
     2341 ggcgaatcga cgaacggcat cgttggggaa acgcgtggtg atggttgtat gtgcaccatc
     2401 gcgcaacaac cgcagcgcga tatacatacc gatcttcgcg cgaccgccgg tcagcaatgc
     2461 gcggcgatca gtcaaatcgg cgcgtgcatc acgacggctg cgattcaacg ccgcgcactt
     2521 cggacacagt tggtgataga aggcgtcgac gtcgcgatag cgttccttgc agatatagca
     2581 agcgcgcgcc tttcccagca ctccgactat cggcgccgtt gcactggcag tgagtgcgag
     2641 cccgcgtgtc tcgtcgtcga tccgtcccgg cgcgccggtt gcggtggccg ccgtgattgc
     2701 ctcgtcggcg gcgacgaccg acgagcgctt ttcctttcgg ttgcgcagct tcacggtctt
     2761 gtagaggcga gcagttgcgc gccgcaccgc gatcgcgtgc ggatg
//
LOCUS       DASWVF010000655         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_375066, whole genome shotgun sequence.
ACCESSION   DASWVF010000655 DASWVF010000000
VERSION     DASWVF010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375066"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VGD55_11570"
     CDS             complement(<1..629)
                     /locus_tag="VGD55_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0871029.1"
                     /translation="MRRLTAVPLSVLALGTAAVGYAAGIERRSPRLRTLEVPVLPEGS
                     SPLRILHFSDAHLTPRQTWKRDWIRSLAAYEPDLVINTGDSIAHVDAVPAFLEAVAPL
                     LDKPGAFVFGSNDFAAPVFKNPAKYLFPDDGGRPKGVELPWRELREAMSDAGWLDLTN
                     ARGYLKADGRQIELVGVGDPHIWEDNYDAVAGSADPGVLLTIGVTHSPEP"
     gene            671..1135
                     /locus_tag="VGD55_11575"
     CDS             671..1135
                     /locus_tag="VGD55_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0871030.1"
                     /translation="MPADTSLKTRLHADLTTAIKGRDELRTATLRMVLSAITNEEVSG
                     AAARQLSDDEVLKVLAREAKKRREAAEAFAAGNRPELAERERAEGGVIDAYLPTQLSD
                     DELRALVSQAVAEAGASGPQAMGAVMKVANPLVAGRAEGGRVAAEVKRQLAG"
     gene            complement(1286..>3440)
                     /locus_tag="VGD55_11580"
     CDS             complement(1286..>3440)
                     /locus_tag="VGD55_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0871031.1"
                     /translation="QAIVAIEDSRFYEHHGIDPKGILRAVVKDTSSGDAVQGASTITQ
                     QYVRQALVETNAQDNNTAAAAAATSKNLSRKLTEARYALALEQKLTKDQILEDYLNTV
                     YFGDGAYGISAAAQHYFNESASDLTLPQSALLAGVINNPSTFDPVLNPKNATARRNIV
                     LDRMLVNYPTSYTAAQIQTAEDAPLGLNVFPIPNGCATATAPFFCDYVVHDILNGSEF
                     GATVTDRANLLRRGGLVVTTTLDPVMQAAAQNAVNKVVPASSSLNAGLAAAIDMVDPK
                     TGAIKAMAIDRPYGSGLGQTEVNLAADYVNHGGSQGRNAGSTFKIFVLAAALAKGIPT
                     GLTINSPGKITIPAGSETTCDGLPITTPWKLVNAEASEGGVFNMVTATAASVNTYFAQ
                     LEVKTGLCAPITIAQDLGVHKATGGDLDQVPAFVLGAEPVDPLSVAGAFASFANNGKF
                     CTPQSITRVTWQVGDVTTNRAVPAPNCGQVLDPAIAEGVTTLLQGVLKAGGTGAGLDI
                     GRPAAAKTGTDDDFKNAWFSGYTPNLAASVWVGNPDSTIPMRAITVNGKKYPEVFGAT
                     LSGPIWQQAMKGALANQPIENFPNAPDLILRGPAIKIPSLQGQDPKAAAAALTNLGLY
                     PTIVATPVTSSEPAGKVEATDPVAGTTVYGGALVKILVSNGIPAVVLSSPPASEPPGS
                     QVPGSPGAPGTIGIGGGTPSPSATVTPSPTGSPSH"
BASE COUNT          551 a         1094 c         1178 g          617 t
ORIGIN      
        1 ggttctggcg aatgtgtgac gccgatcgtc agcagcacac ctggatcagc gctacccgca
       61 acggcgtcgt agttgtcctc ccagatatgc ggatcaccga cgccgacgag ttcgatctgg
      121 cgcccgtcag ctttcaggta cccgcgcgca ttggtcaggt cgagccaccc ggcgtccgac
      181 atcgcttctc gaagttcacg ccacgggagc tcaacacctt ttgggcgacc accatcatcg
      241 gggaagaggt atttcgctgg attcttgaag accggtgccg cgaaatcgtt cgagccgaag
      301 acgaatgcac ctggcttgtc gagcagcggc gcgacagctt cgagaaacgc cgggaccgca
      361 tcgacgtggg cgatgctgtc gccagtgttg atgaccaggt cgggttcgta cgcggcgagg
      421 ctgcgaatcc agtcgcgctt ccaggtctgc ctgggcgtga ggtgcgcgtc cgaaaaatgc
      481 agtatccgca gcggactcga gccctccggc agcaccggca cctcgagcgt tcgcaaccgt
      541 ggcgagcgcc gttcgatccc cgcggcatac ccgacagctg cagtaccaag ggcaagaacg
      601 ctgagcggga cggcggtgag tcggcgcata gcgtcaagtt tcgcagacgc cgcggtgaga
      661 cactggaccc atgcccgctg acacgtcact caagactcgc ctgcacgctg atctgaccac
      721 cgcgatcaag ggccgcgacg agttgcgcac ggccaccttg cgtatggtcc tttcggcgat
      781 cacgaacgaa gaggtatcgg gcgcggccgc tcgccagctt tccgacgacg aggtgttgaa
      841 ggtgctcgcg cgtgaggcga agaagcgccg ggaggccgcg gaggcgttcg cggcgggcaa
      901 ccgtccggag cttgcggagc gcgagcgcgc tgagggcggc gtgatcgatg cctaccttcc
      961 gactcagcta tctgacgacg agctgcgcgc gctggtctcg caagccgttg ccgaggcggg
     1021 cgcatccggg ccacaggcga tgggtgctgt gatgaaggtc gcgaacccgc tggttgcggg
     1081 ccgggccgag ggtggccgag tcgcggctga ggttaagcgc caactcgccg gctgaccgcc
     1141 gcccgcgcac ccgcgcaaga ggagcccgaa ggttcgcggc gacggcccgg atcgtggagt
     1201 gtggcgatcg ctagaggtgc cttggcactc cacgctaatc cgagggcggc cgcctcgccc
     1261 gccggccgac cgtcgtcgcg ccgagctagt gcgacgggga gccggttggg ctcggcgtca
     1321 ccgttgcgct cgggctcggc gttcccccgc cgatgcctat ggtcccaggc gccccgggcg
     1381 agcctggaac ctgcgagccc ggcggctccg acgccggagg cgacgacaac acgacggccg
     1441 gtatcccgtt gctgaccagg atcttcacga gcgcgccgcc gtacaccgtc gtgccggcca
     1501 ccggatcggt ggcttcgacc ttgcccgccg gctccgacga cgtcacgggc gtggcgacga
     1561 tcgtcgggta gaggccgagg ttcgtgaggg ccgcggcggc cgccttcgga tcctgtcctt
     1621 gcaggctcgg gatcttgatc gccggcccgc gcaggatcag gtcgggtgcg ttcgggaagt
     1681 tctcgatcgg ctggttcgca agcgcgccct tcatcgcttg ctgccagatg gggcccgaca
     1741 gcgtcgcgcc gaacacctcg ggatacttct tgccgttcac cgtgatcgcc cgcatcggga
     1801 ttgtcgagtc ggggttgccg acccacaccg acgcggcgag gttgggcgtg tagccgctga
     1861 accaggcgtt cttgaagtcg tcgtcagtac cggtcttggc ggcagccggg cggccgatgt
     1921 cgagcccggc gccggtgcca ccggccttca gcacaccctg caagagggtc gtgacgccct
     1981 cggcgatcgc cgggtcgagc acctgtccgc agttcggcgc cggtaccgct cggttggtgg
     2041 tgacgtcgcc gacctgccaa gtgacgcggg tgatcgactg cggggtgcag aatttgccgt
     2101 tgttcgcgaa cgacgcgaaa gcacccgcga cgctgagtgg gtcgaccggt tctgcgccga
     2161 gcacgaaggc cggaacttgg tcaaggtcac caccagttgc tttgtggacg ccgaggtcct
     2221 gggcaatcgt gattggcgca caaagacccg tcttcacctc gagctgcgcg aagtaggtgt
     2281 tcaccgaagc ggcagtcgcg gtgaccatgt tgaacacgcc accctcggac gcctcggcgt
     2341 tgacgagctt ccacggcgtc gtgatcggca ggccgtcgca cgtggtctca ctgcccgcgg
     2401 ggatcgtgat cttccctggt gagttgatcg tcaggccggt cggtatcccc ttcgccagtg
     2461 cagcagccag cacgaaaatc ttgaacgtcg agccggcgtt gcggccctgc gaaccaccgt
     2521 ggttgacgta gtcggccgcc aggttcacct cggtctggcc gagccccgag ccatacggcc
     2581 ggtcgatagc cattgctttg atggcgccgg tcttcgggtc gaccatgtcg atcgccgcgg
     2641 caagcccggc gttgagactc gatgatgccg ggacgacttt gttgaccgcg ttctgtgccg
     2701 ccgcctgcat caccgggtcg agcgtcgtcg tgacgacgag accaccgcga cgcaacagat
     2761 tcgcccggtc ggtgaccgtg gcaccgaact cggatccgtt caggatgtcg tgcacgacgt
     2821 agtcgcagaa gaacggggcc gtcgcggtgg cgcagccgtt cggaatcgga aacacattca
     2881 gcccgagcgg agcatcctcc gctgtctgga tctgggccgc cgtatagctc gtggggtagt
     2941 tgaccagcat tcgatcgagc acgatgttgc ggcgggcagt tgcattcttc ggattcagca
     3001 ccgggtcgaa tgtgctgggg ttgttgatga ctccggcgag caatgcagac tgcggcaacg
     3061 tcaaatcgga cgccgactcg ttgaagtagt gctgggcggc ggccgaaatg ccgtaagcgc
     3121 cgtcgccgaa gtacaccgtg ttgaggtagt cctcgaggat ctggtctttc gtcagcttct
     3181 gctcgagcgc cagtgcgtac cgtgcttcgg tgagtttgcg cgacaggttc ttcgacgttg
     3241 cggcagccgc cgcagccgtg ttgttgtctt gggcgttggt ctcgaccagc gcctggcgca
     3301 cgtactgctg agtgatcgtc gaggcaccct gcaccgcatc gccagacgac gtgtctttca
     3361 cgactgcccg caggatgccc ttcgggtcga tcccgtggtg ctcgtagaaa cgggagtctt
     3421 cgatggccac gatcgcctgc
//
LOCUS       DASWVF010000656         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_196946, whole genome shotgun sequence.
ACCESSION   DASWVF010000656 DASWVF010000000
VERSION     DASWVF010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196946"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..429
                     /locus_tag="VGD55_11585"
     CDS             244..429
                     /locus_tag="VGD55_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEY0871032.1"
                     /translation="MADYEFRQLLLPRGTSRNAAWRLLTEQAEHHGWELDRLRLYPDG
                     TRRVRLRRRILRVARTL"
     gene            complement(451..>1569)
                     /locus_tag="VGD55_11590"
     CDS             complement(451..>1569)
                     /locus_tag="VGD55_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0871033.1"
                     /translation="VTRIEGTHPGRGALLIHGHLDVVAADPAHWKVDPFAGEIIDGCL
                     WGRGAIDMKDMDAMSLAVIRQWMREGRQPPRDIVLAYVADEEAGGRKGAHWLVDRHPE
                     LFDGCTESISEVGGFSIQIRDDLRLYPVQTAQRGMAWMKLVARGRAGHGSLVNHANAV
                     TELAEAVARIGRYEWPVRLTKTVEAFLDELGQAIGVELDPYDLGAVAGKLGTITALIA
                     ATVSNTANPTMLEAGYKVNVIPQEASAHIDGRFLPGHEEEFYATIDELLGPNVTRETI
                     NADIAIETEWDGALVEAMKASITSEDPQGKAIPYCLFGGTDDKSFSLLGIRGVGFSPL
                     RLPPDLDFAGMFHGVDERVPVEGLKFGTRVLDHFLSNC"
BASE COUNT          262 a          491 c          519 g          297 t
ORIGIN      
        1 ggccgcttgg acgtgcggat cgttctcgcc agctcgtgac tcgacggcgg cgaggtgggc
       61 gtcgagggcg gatttcaaac cggccacggc ggcgcgaacc tgctctggcg tggcggagaa
      121 ctcgtcgggc atggggtgaa cgctaccggc ccacccccat ggacgcgcaa cgtcgatctt
      181 ttggggccga gatttggacg caaatgtgag gccgcgcgga ccttgatgcg agcacaatga
      241 accatggccg actacgaatt caggcagctg ctactgccgc gtgggacgtc gcgcaacgct
      301 gcttggcggc tgctgaccga gcaggcagaa caccacggct gggaactcga ccgcctgcgc
      361 ctttaccccg acggcacccg acgggtgcga ctgcgtcgac ggatcctgcg ggtcgcgcgg
      421 acgttatagg cagctagcag ttcgtcaaac tcagcagttc gacagaaagt ggtcgagcac
      481 cctcgtaccg aacttcaggc cctcgaccgg cacccgctca tcaacgccgt ggaacatgcc
      541 ggcgaaatcg aggtcgggtg gcagccgaag tggcgagaag ccgactcccc tgatgccaag
      601 cagcgagaac gacttgtcat cggtgccacc gaacaggcag tacgggatcg ctttgccctg
      661 aggatcttct gaggtgatgg atgctttcat cgcctcaacc aacgcgccgt cccactctgt
      721 ttcgatcgcg atgtcggcgt tgatcgtctc gcgcgtcacg ttgggcccga ggagttcgtc
      781 gatggtggcg tagaactctt cttcatggcc agggagaaag cggccgtcga tgtgcgccga
      841 tgcctcttgc ggtatcacgt tgaccttgta acccgcctcg agcatcgtcg ggttggctgt
      901 gttgcttacg gtcgctgcaa tgagcgcggt gatcgtgccg agcttgcctg caaccgcgcc
      961 gaggtcgtac gggtctagct cgacgccgat cgcttggccg agttcgtcga ggaatgcttc
     1021 gactgtcttc gtgagacgca ccggccattc gtagcggccg atccgtgcga ctgcctccgc
     1081 gagttcggtg acggcgttcg cgtggttgac caggctcccg tgtcctgcgc ggcctctcgc
     1141 gacgagcttc atccatgcca tgccgcgctg cgcagtctgt acaggataga gccgcaggtc
     1201 gtcgcggatt tgaattgaga agccgccgac ctcactgatc gactcagtgc atccgtcgaa
     1261 cagttcgggg tgacggtcga cgagccaatg cgcgcctttg cgaccgccgg cctcttcgtc
     1321 ggcgacgtag gcgagcacga tgtcgcgcgg tggctggcgg ccttcgcgca tccactgtcg
     1381 gatcacggcg agcgacatcg cgtccatgtc cttcatgtcg atcgcgccgc ggccccatag
     1441 gcagccgtcg atgatctcgc ccgcgaacgg atcgaccttc cagtgcgccg ggtcggcggc
     1501 gacgacatcg aggtggccgt ggatcaacag cgcgccgcgg cctggatggg tgccttcgat
     1561 gcgggtgac
//
LOCUS       DASWVF010000657         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_451968, whole genome shotgun sequence.
ACCESSION   DASWVF010000657 DASWVF010000000
VERSION     DASWVF010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451968"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="VGD55_11595"
     CDS             <1..1827
                     /locus_tag="VGD55_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0871034.1"
                     /translation="SRARRCITDHPPGTRAFSGHIIVCGLEGIGVTIVEQLHRSGQQV
                     VVLEQFTKPAQLASVSGWITAVIASKGSLATTLDAAGITAASAIVCVVAQDLQNVQIA
                     LQARHMSAHIRVVSQLGNNAVRRAMAVDNGPGAVIDGASLAAPAIVEACLGRRLHEMQ
                     FGTTTFVAATVPVAGGTLRSAFGDLAPVAVTRQQPNDTQPTVFACPGRDFVTMPGDYA
                     TMLGTPDELRMRGIEFATNAALGETPHRAGRLRQLPGAVRAAIGEFDPGVYRALIALL
                     TLVTISTLLLHAGYRKPGMSVLDALYFSVETVATVGFGDFSFAQQDSLLRIWAIFLML
                     AGITTTAVLMAYVADFLISRRLNHSIGRRRARGMSGHVIVVGLGAFGLQVAARLQAVG
                     KQVVVIERDERNRFLSEAATLDLPIIFGDATSTVTLESAQLRHAAAIAILTSDDMVNI
                     ETGIAVRDLLDDQWTVGSLPVVMRVFDQSLSRTISARFGFQNIQSIEEITAPWFVAAA
                     LGLEVLGAFSVADQPFTIGRITIAADSGLVGMAMQGLSANTRVIALHRHATGVLEHPP
                     RRGTRFDEGDLAYIVGPNDELIGVLRTATGATVSLDQVPEVV"
BASE COUNT          302 a          630 c          592 g          338 t
ORIGIN      
        1 agccgggcgc ggaggtgcat cacggatcac ccaccgggga cccgtgcatt cagcgggcac
       61 atcatcgtct gcggcctcga aggcatcggc gtcaccatcg tcgagcaact gcaccgaagc
      121 ggccaacagg tcgtggtcct ggagcagttc accaagcctg cccaactcgc gtcggtgtcg
      181 ggctggatca ccgctgtgat cgcgtcgaag ggcagcctcg ccacaacgct cgacgccgcc
      241 ggcatcactg ccgcaagcgc gatcgtgtgc gtcgtcgcgc aagacctcca aaacgttcag
      301 atcgctctgc aagcccggca catgagtgcg cacatcaggg tcgtctcgca gctcggaaac
      361 aacgcagtgc ggcgcgccat ggcggtcgac aacggaccag gcgcggtcat cgacggcgcc
      421 tccctggcag cccccgcaat tgtcgaagcc tgcctcggac gtcgattgca cgagatgcag
      481 ttcggcacga ccaccttcgt cgcggcgact gttcccgtcg cgggcggcac cttgcgttcg
      541 gccttcggcg acctcgctcc ggtggccgtc acccgccagc aacctaacga cacgcagccg
      601 accgtgttcg cctgcccggg ccgtgatttc gtgaccatgc ccggtgacta cgccacgatg
      661 ctcggcacac ccgatgagct ccgcatgcgc ggaatcgagt tcgcgacgaa tgccgccctg
      721 ggcgaaaccc cgcatcgggc tgggcgactc cggcagttgc cgggcgcggt gcgtgcggcg
      781 atcggtgaat tcgaccccgg cgtctaccgt gcgctgatcg cgttgctgac tctcgtcacc
      841 atctctacgc tgctgctgca cgcgggttac cgcaagcccg gcatgagcgt gctcgatgcc
      901 ctctatttca gcgtcgaaac cgtcgcgacc gtcggcttcg gtgatttcag cttcgctcag
      961 caagactcgc tgctgcgcat ctgggccatc ttcttgatgc tggccggcat caccacgacg
     1021 gcggtcctga tggcgtacgt ggccgacttc ctgatcagcc gtcgcttgaa tcattcgatc
     1081 ggacgccgac gggctcgggg tatgtccgga cacgtgatcg tcgtggggct tggggcgttc
     1141 ggtctgcagg tggccgcccg tttgcaggct gtcggcaagc aggtcgtggt catcgaacgc
     1201 gacgagcgca ataggttctt gtccgaggcc gcgacacttg acctgccgat catcttcggc
     1261 gacgcgacgt cgacggtgac ccttgaatcg gcgcagctgc gccatgctgc ggccatcgcg
     1321 attctgacca gtgacgacat ggtgaacatc gagaccggca tcgccgtgcg tgacctgctc
     1381 gatgaccaat ggacggtggg gtcgcttccg gtcgtgatgc gagtcttcga ccagtcgctg
     1441 tcgcgcacga tctcggcccg cttcggcttt cagaacatcc agtcgatcga agagatcacg
     1501 gcgccgtggt tcgtggcggc cgcgctgggg ctcgaggtgc tcggggcgtt ctcggtcgca
     1561 gaccagccgt tcaccatcgg ccgtatcacg atcgctgccg acagcggcct ggtcggcatg
     1621 gcgatgcagg gcctctccgc caacacccgg gtgatcgcac tgcatcgcca tgcgactggc
     1681 gtgctcgaac acccaccgcg tcgcggaacc cggttcgacg aaggcgatct cgcctacatc
     1741 gtcggcccca acgacgagct gatcggcgtg ctccgcaccg cgaccggggc gaccgtcagc
     1801 ctcgaccaag tgcccgaggt cgtctaggtg tatgcttgcc gataggcaac tatctgtgag
     1861 ga
//
LOCUS       DASWVF010000658         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_145867, whole genome shotgun sequence.
ACCESSION   DASWVF010000658 DASWVF010000000
VERSION     DASWVF010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145867"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VGD55_11600"
     CDS             <1..905
                     /locus_tag="VGD55_11600"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0871035.1"
                     /translation="RGIGPAYSDKMSRIGVRVQDLFDEKILRQKVEGALLPKNQLLVK
                     IYNRAAINTSAVVTELLEYADRLKPMVQDTSLLLNNALDEGKTVLLEAGQATLLDVDH
                     GTYPFVTSSNATAGGACTGSGIGPTRIDRVIAVVKAYTTRVGSGPFPTELEDADGEKL
                     RSIGAEFGTTTGRPRRCGWYDAVIARYATRVNGVTDFVLTKLDVLSDFDRIPVCVGYR
                     LPDGSVIDEMPMTQTDFHHAQPVYEEFPGWREDISEAREFDDLPKNDRKYVEALQDMS
                     GAPFSVIGVGPARDQSLQLRPLTP"
     gene            902..2155
                     /gene="purD"
                     /locus_tag="VGD55_11605"
     CDS             902..2155
                     /gene="purD"
                     /locus_tag="VGD55_11605"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0871036.1"
                     /translation="MNVLVIGGGAREHAICLALRNDPSVTTLHCAPGNAGIAAIAAVH
                     EVDVHDPAAVAELALQLTAELVVIGPENPLVAGVVDAVQAKGIACFGPTRAAARLEGS
                     KAFAKEIMAAADVPTAASRVCVDARQVAAALDEFGPPYVVKDDALAAGKGVVVTLERD
                     VALEHASHCGRVVIEEYLDGPEVSLFAITDGTTVVPLIPAQDFKRLADGDHGPNTGGM
                     GAYAPLPWAPPTLIDDMMRDVLRPTIDEMRRRGTPFAGLLYAGIALTSKGVRVVEFNA
                     RFGDPETQVVLALLKTPLASLLQAAATGTLADHPPLEWHAGFAITVVIAAKGYPGQPV
                     NGDPIGGLDGIPAPAYVLHAGTAADASGQVVSAGGRVLSVTATGDTLEAARDAAYAGV
                     DQITLEGAQYRSDIALLAVRGEITA"
     gene            2218..2739
                     /locus_tag="VGD55_11610"
     CDS             2218..2739
                     /locus_tag="VGD55_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY0871037.1"
                     /translation="MRATDEAEFKAYAAARMRDLRRTAFLLCGDWHHADDVVQTVFTK
                     LYVNWDKVQRHDRLDGYVKTMLVRATFDRRRRMSWRRELSSASPPETPTPTPSNVEDR
                     LLLFDALAKMPPRQRAVVVLRFWQDLDVTETAHLLGCSEGTVKSQSARGLAKLRELLA
                     PADSEPSMEGQPR"
     gene            2736..3590
                     /locus_tag="VGD55_11615"
     CDS             2736..3590
                     /locus_tag="VGD55_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871038.1"
                     /translation="MNDEDFARRAFAAAFRSGQTGEPPTLPDVELLALHGRRANRYRH
                     GAYAVGTTVLAGVTVVGVVGGPALLGLGSNSPSGISVGAGAPAAPAPSSSSSAPDPAK
                     PSSGVPCATPPAINWASVLAGALPAGVTATADHLANCVQLPDGTRSVEALFKLSTGNV
                     GLQVDVATGPEIARKLTGGAGKIGADASPEPGDSPSPNPSGSTGSKGAAAGNCSKVGP
                     DENACISRLTKDSASVVDVQILRTGTSPIVVDVAASNGKNLATPDTGQLPSDATMLAI
                     AEAVAAHF"
BASE COUNT          707 a         1227 c         1199 g          598 t
ORIGIN      
        1 gccgcggcat cgggcccgcg tactccgaca agatgtcgcg catcggcgtc cgggtccagg
       61 acctcttcga cgagaagatc ctgcgccaaa aggtcgaagg cgccttgctg ccgaagaacc
      121 agctgctcgt gaagatctac aaccgcgcag ccatcaacac gagcgccgtc gtgaccgaac
      181 tgctcgagta cgccgatcga ctcaagccga tggtgcaaga caccagcctg ctcctgaaca
      241 acgccctcga cgagggcaag accgtgctgc tcgaggccgg ccaggcaaca ctgctcgacg
      301 tcgaccacgg cacgtatccg ttcgtcacgt cctcgaatgc aacggctggt ggtgcctgca
      361 cgggttcggg catcggaccc acccgcatcg accgggtcat cgccgtcgtg aaggcgtata
      421 cgacccgcgt cggcagcggt cctttcccta cagaacttga agatgcggac ggcgagaagt
      481 tgcgctccat cggagcggaa ttcggcacca caactgggcg tccgagacgt tgcggctggt
      541 acgacgcggt catcgcgcgc tatgccacgc gggtcaacgg cgtcaccgac ttcgtgctga
      601 ccaagctcga cgtgctgtcg gacttcgatc gaatcccggt gtgcgtcggc tatcggctac
      661 ccgacggcag cgtcattgac gagatgccga tgacgcagac cgacttccac catgcgcagc
      721 cggtctacga agaattccct ggctggcgcg aagatatctc cgaagcacgg gagttcgacg
      781 acctaccgaa gaacgaccgc aaatacgtcg aggcgttgca agacatgtcc ggcgcgccat
      841 tcagtgtgat cggcgtgggt ccggcgcgcg accaatcctt gcagttgcgc ccactgacgc
      901 cgtgaacgtc ctcgtcatcg gtggcggtgc gcgcgagcat gcaatttgtc tcgccctgcg
      961 aaatgacccg agtgtgacga ccctgcactg tgctcctgga aacgcaggca ttgcggcaat
     1021 cgcagccgtt cacgaagtcg acgtgcacga ccctgcggcg gtagctgagc tagctctaca
     1081 actcaccgcc gaactggtgg tgatcggccc agaaaaccct ttggtagcag gggttgtcga
     1141 cgcggtacaa gctaaaggta tcgcgtgctt cggacccacc cgagcggccg cacggctcga
     1201 agggagtaag gcgttcgcga aggaaataat ggctgccgcc gacgtcccga ctgctgcgtc
     1261 gagggtgtgc gttgacgcca gacaggttgc tgccgcactc gatgagttcg gtccgccgta
     1321 tgtcgtgaaa gacgacgcac tcgcggccgg caagggagtc gtcgtcaccc tcgaaagaga
     1381 cgtcgcgctc gagcacgcga gccattgcgg gcgcgtcgtc atcgaggaat acctcgatgg
     1441 tcccgaggtg tcgctgttcg cgatcaccga cggcacaacg gtggttccgc tcatcccagc
     1501 acaagacttc aagcgcttag cggacggcga tcacggaccg aataccggag gcatgggcgc
     1561 ctacgcacca ctgccatggg cgccgcccac cctgatcgac gacatgatgc gcgacgtcct
     1621 tcggccgacg atcgatgaga tgcgcaggcg aggcaccccg ttcgcaggcc tcctctacgc
     1681 aggcattgcg ttgacatcga agggcgttcg agtcgtcgag ttcaacgcac gtttcggaga
     1741 cccggagacg caggtcgtgc tggcgttgct caagacgccg ctggcatcac tgctccaggc
     1801 cgccgccacc ggcacgctgg ccgatcatcc gccgctggag tggcatgcgg gattcgcgat
     1861 caccgtggtg atcgcggcca agggctatcc cggacagccg gtcaacggcg acccgatcgg
     1921 cggactcgat ggcattccgg cacccgccta cgtgctgcac gcgggcaccg ccgccgacgc
     1981 atcgggccag gtggtctcgg cgggcggacg cgtgctgtcg gtcaccgcga caggagacac
     2041 cctggaagcc gctcgcgacg cggcctacgc cggcgtcgat cagatcaccc tcgagggcgc
     2101 tcagtatcga agcgacatcg cactcctcgc cgtccgggga gaaatcacgg cctaggtgca
     2161 acctggcacg ccagcccaca cctgtaggag ggtgcaggca acgcgagagg accaggcgtg
     2221 cgagcgaccg atgaggctga gttcaaggcc tacgccgctg cccgcatgcg cgatcttcgt
     2281 cgcacggcgt tcttgttgtg cggcgattgg caccacgccg acgacgtcgt gcagaccgtt
     2341 tttaccaagc tgtatgtgaa ttgggacaag gtccagcggc acgaccggct cgacggctac
     2401 gtgaagacga tgctcgtgcg cgccactttc gaccgacgac gccgcatgtc gtggcgacgt
     2461 gaactgagct cagcgtcacc acccgaaacc ccaaccccaa ccccaagcaa cgtcgaagac
     2521 cggctgctct tgttcgacgc gctcgcgaag atgccaccaa gacaacgtgc cgtcgtggtg
     2581 ctgcgcttct ggcaggacct cgacgtgacc gaaaccgcac acctgctcgg ctgtagcgaa
     2641 ggaactgtga agagccaaag tgctcgcggg ctcgcgaagt tgcgcgaact cctcgcaccc
     2701 gctgatagcg agccctcgat ggaaggacaa ccacgatgaa cgacgaagac tttgcccggc
     2761 gcgcctttgc ggccgcgttc cgctccggcc aaacgggaga accgccaact ttgccggacg
     2821 tcgagttgct ggcgctgcac ggaaggcggg cgaatcgcta ccggcacggc gcctacgccg
     2881 tcggcacaac cgtgctggcc ggggtaaccg ttgtcggcgt ggtgggcggt ccggcgctgc
     2941 tcgggctcgg cagcaactcg ccgtccggca tctcggtagg agcaggcgca cccgcggcgc
     3001 ctgcgccgtc atcttctagt tcggcacctg atcccgcaaa gccatcttct ggggtcccgt
     3061 gcgccacccc gcccgcgatc aactgggcaa gtgtgctggc gggcgcccta ccggcgggtg
     3121 tgactgcgac ggccgatcac ctcgcgaact gcgtccagct ccccgatggc acgcgaagtg
     3181 tcgaagcctt gttcaagctc tcgacgggga acgtcggact ccaagtggac gtggccaccg
     3241 ggcccgagat tgctcggaag ctgactggcg gggcagggaa aattggtgct gacgcgagcc
     3301 cggagccggg agactcgccg tcgccgaacc cttcaggatc cacggggagc aagggggcgg
     3361 ctgccggaaa ctgctcgaag gtcggcccgg acgaaaacgc ctgtatctca cgcctcacca
     3421 aggattcggc gtccgttgtc gacgtccaga tcctgcgcac cggcacaagc ccgatcgtgg
     3481 tggacgtcgc ggcaagtaat ggaaagaacc tcgcgacacc cgacaccggg caactcccga
     3541 gtgacgcgac gatgctggcc atcgccgagg ccgtggccgc tcacttctaa gggtcaggca
     3601 cgggcggtgc gagaatggtc gggtcatgac aaacgcgccg cagattcccg acgtgctggc
     3661 caccaggtac gtctcccccg aggtggtgca gctctggtcg cctgcagcca aggtgattct
     3721 cgagcggcgc c
//
LOCUS       DASWVF010000659         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_273541, whole genome shotgun sequence.
ACCESSION   DASWVF010000659 DASWVF010000000
VERSION     DASWVF010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273541"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VGD55_11620"
     CDS             complement(<1..1590)
                     /locus_tag="VGD55_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871039.1"
                     /translation="MTAASAAQRLYDPAHPARTAPRLYSGQRKRLTLAAIAFAFKQSP
                     VWITPLLTANIIDAVVSRRPVSVIWENAAVMGGLIVVNAPLTWAYVRWLSLALRTVEV
                     ESRQALCERLQQLSIGYHKRMSAGVLQTKVIRDVETIVETSRQTFDSGMAASTTLVGA
                     LVLTAWRVPEFLPVFLIAVPASAGLVHTMRRRMSDRNKAFRKQVDQLSARVSEMTQLI
                     PVTRAHALEQDELERIDSTLYGVRDQGVRLDVLNGRFNAFAWVLFQLLSVSCLVIAAW
                     GARTGALKVTPGDVVLLSTYFVTLTGSVTMLLNVAPLVSKGIESMRSMAEVLTDPDLE
                     INAGKRRVSRVHGRVTFENVAFTYPDAAEPALVDICLDVQAGETVALVGPSGSGKSTM
                     LSLVIGFLTPTHGRLLLDGEDRRDLDLRGYRRFLAVVPQETLLFDGTVRDNVTYGQSD
                     LAEARVRDALEAANAWDFVQAMGGLDAMVGQRGGQLSGGQRQRLAIARALIRDPRVLV
                     LDEATSALDNASETLVQDAPAR"
     gene            1653..>2310
                     /locus_tag="VGD55_11625"
     CDS             1653..>2310
                     /locus_tag="VGD55_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0871040.1"
                     /translation="MARSGFNLQWLYSKRAEPRGPDELVLDAIAAWGFDFVRLPTDYR
                     LWSRDDDPYRPDERVLALIDDVLIACQSRGIHLSLNLHRAPGYVITGWETEPYNLWAD
                     EPAQDAFVATWERFAQRYETVAADDLSFDLVNEPPALGLRGFTREAHEAVIRRTVASI
                     RAISAGRPLVIDGLDGGNLAMPELADLGLTQSVRGYQPMRVSHYEAPWWPGHIGMPEP
                     A"
BASE COUNT          412 a          782 c          732 g          384 t
ORIGIN      
        1 cctggcgggc gcgtcttgca caagagtctc cgacgcgtta tcgagcgccg acgtcgcctc
       61 gtcgagcacg agcacccgcg ggtcgcggat aagggcacgc gcgatcgcaa gtcgttgccg
      121 ctgcccaccg gaaagctgac cgccgcgctg cccgaccatg gcgtcgagcc cgcccatcgc
      181 ctgaacgaaa tcccatgcgt tcgcggcttc gagggcgtcg cgcacccgcg cctcggcgag
      241 atcggactgt ccgtaggtga cgttgtcgcg caccgtaccg tcgaacagca gcgtctcctg
      301 cgggacgacc gcgaggaacc tgcgataccc gcgcaagtca agatcgcgcc gatcctcgcc
      361 atcgagcagc aggcggccgt gggtcggcgt caggaatccg atgacgaggc tgagcatcgt
      421 cgactttccc gatcccgaag gcccgaccaa cgcgactgtc tcgcccgcct gtacgtcgag
      481 gcagatatca acgagcgccg gctccgcggc gtccggataa gtgaacgcca cgttctcgaa
      541 cgtgacgcgg ccgtgcacgc gagagacgcg ccgcttcccc gcgttgatct cgaggtcagg
      601 gtctgtcaga acttcggcca tcgagcgcat cgactcaatg cccttcgaca ccagcggagc
      661 gacgttcagc agcatcgtca ccgagccggt cagcgtgacg aaatacgtcg acagcaggac
      721 gacgtcgcct ggcgtcacct tgagcgcacc ggtccgggca ccccaagcgg cgatcaccag
      781 gcagctgacg ctcagcagtt ggaaaagcac ccaggcgaac gcgttgaacc gcccgttcaa
      841 gacgtcgagg cggacgcctt ggtcgcgcac gccgtacagc gtcgagtcga tcctttcgag
      901 ttcgtcttgt tcgagcgcgt gcgcgcgcgt gacgggaatg agctgcgtca tctcgctgac
      961 gcgcgccgac agttgatcga cctgctttcg gaatgccttg ttgcggtcgc tcatgcggcg
     1021 ccgcatggtg tgcacgaggc cggccgatgc gggcacggcg atgaggaaga ccggcaggaa
     1081 ctccggcact cgccacgcgg tgagcaccag agcgccgaca agcgtggtgc tcgccgccat
     1141 tccggaatcg aaggtctgtc gcgacgtttc cacgatggtt tcgacgtcgc gaatcacctt
     1201 tgtttgcagc actccggcgc tcatccgctt gtggtagccg atcgacaact gctgaaggcg
     1261 ctcgcacagc gcctgccgcg attcaacttc gaccgtgcgc aacgccaggc tcagccaccg
     1321 aacataggcc catgtcagcg gcgcgttcac cacgatcagt ccgcccataa ccgctgcgtt
     1381 ctcccagatc accgataccg gacggcgaga tacgaccgcg tcgatgatgt tggctgtcaa
     1441 cagcggtgtg atccacaccg gcgactgctt gaacgcgaag gcgatggctg ccaacgtcag
     1501 ccgtttgcgc tgacccgagt acagccgagg agcggttctt gccgggtgcg ccgggtcgta
     1561 gagacgttgc gccgcgctcg cagcggtcat gtccgacgca cctcatgact catcgactta
     1621 tgcgcttcag ttccgagaga aaggtactcc acatggcgcg gtcgggtttc aacctgcaat
     1681 ggctatacag caagcgggcg gagccgcgcg gccccgatga gttggtgctc gacgcgatcg
     1741 ctgcctgggg cttcgacttc gtgcgattgc ccacggacta ccggttgtgg agccgtgacg
     1801 acgaccccta ccgacccgat gagcgcgtgc tcgcgctcat cgacgacgtc ctcatcgctt
     1861 gccagtcgcg cgggatacac ctgtcgctga accttcatcg cgcacccggc tatgtcatca
     1921 cgggctggga aacagagccc tacaacctgt gggccgacga gccggcgcaa gacgccttcg
     1981 ttgccacctg ggaacggttc gcgcagcgct acgagacggt cgctgccgac gacctcagct
     2041 tcgacctggt gaacgagccg ccggcgctag gtctgcgcgg cttcactcgc gaggcgcatg
     2101 aagcggtgat caggcgcacc gtcgcgtcga tccgcgcgat ctcagcgggg cgcccgctcg
     2161 tcatcgacgg cctcgacggt ggcaacctcg cgatgccgga actcgcagac ctggggctga
     2221 cccaaagcgt ccgcggttac caaccgatgc gcgtgtcgca ctacgaggca ccctggtggc
     2281 ctgggcacat cggcatgccc gaaccggcgt
//
LOCUS       DASWVF010000660         6902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_171015, whole genome shotgun sequence.
ACCESSION   DASWVF010000660 DASWVF010000000
VERSION     DASWVF010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171015"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VGD55_11630"
     CDS             <1..1172
                     /locus_tag="VGD55_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0871041.1"
                     /translation="FEESAPALRAVSEWLTGMGGRIDTITDQLREVGDAASRIGRIAT
                     QTHILALNASIEATKNVTDGRGFAVIAESVRTLASESAASAVSINTTLGALATSLDVF
                     RSDSAEADAHARSVQASTDAMSEFISELTGRAARVSDSAQTIEDTATASQEGTSHFVD
                     SLSRLTEGIGRSQKEIGRSRDRVASLLGSANVVLQDLSASGVRTVDTPYIDAAVNAAA
                     RAKAIFEDAVARGAIAREDLFDENYVPIPGTDPLQLLTKFTGFAEKSVLPLMEEVHAS
                     LPASVYCVAGDRNGYAPANTKEYSHPQGPDPAWNESHCRNRRIFDNDVATRAAKNTAG
                     PLIQVYRRDLGGGTFDIVKDAAAPIFVDGRHWGAFRIGYIAGGAESSSAASSSSD"
     gene            complement(1118..1507)
                     /locus_tag="VGD55_11635"
     CDS             complement(1118..1507)
                     /locus_tag="VGD55_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0871042.1"
                     /translation="MADADDVRRLALALPHVEEIKSDGFDFRVGGKGFVWSYPERRPG
                     ERRRIRTDIAVLFVGDEAEKQALLLGEPDVFFTTSGYDGFPLVMLWLEKVDVDRLAEL
                     VTDAWRMRAPQSLLDDAALDDSAPPAM"
     gene            complement(1548..1844)
                     /locus_tag="VGD55_11640"
     CDS             complement(1548..1844)
                     /locus_tag="VGD55_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871043.1"
                     /translation="MDGKPFGVVHFFTGGTKDQYEASIAAVHPADGLPAGQLFHAAGA
                     SEGGWTIMAVHESKESWENFRDGTLMPRMQQGIEGGFAAPPVETEVDLYNVVSD"
     gene            1935..2384
                     /locus_tag="VGD55_11645"
     CDS             1935..2384
                     /locus_tag="VGD55_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0871044.1"
                     /translation="MTTDDVERWTPAWLALIRTHARLWDQVEAQMRHDSGLTLPRYDV
                     LMQLDRSGGRLRLTELASSVVLSPSGLSKLLDRMEASELLRREPDPTDARSTLARLTP
                     RGRLLVRKARQTHHAWLQKAFGDALNERDVADLTRIMTRLDAQISSA"
     gene            complement(2394..4043)
                     /locus_tag="VGD55_11650"
     CDS             complement(2394..4043)
                     /locus_tag="VGD55_11650"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEY0871045.1"
                     /translation="MNYEAAAAQWLAEDPDPDTRAELSALLAGGDAEGLADRFSARLQ
                     FGTAGLRGALGAGPNRMNRAVVIRAAAGLAAYLRATGGTSVVIGYDARHKSDVFARDT
                     AAVMEGAGITASVLPRALPTPVLAFAIRYLGVSAGVMVTASHNPPQDNGYKVYLGDGG
                     QIVPPADAAISQHIDAVGPLADVPRTDAWTVLDESVLHAYLTGITSLPLLVALPHRDV
                     RIAYTPLHGVGRDVVLAAFTRAGFTSLSVVAEQGAPDPDFPTVAFPNPEEPGAIDLAL
                     ALARSTSADLVLANDPDADRCAVAIPNPRVEGGWRTLRGDEVGVLLADFLLTQGISGR
                     YATTIVSSSLLSQMAARHGMAYSETLTGFKWIVRNGDRVYGYEEALGYCVAPSLARDK
                     DGISAALIVALLAATPKADGSTLADRLDDIARSYGVYATDQVSIRVSDLSQIGAAVDR
                     LRADPPSSLGGLPVVGFDDLAVGANGLPPTDGLRFRLDGSALGIGPARVVVRPSGTEP
                     KLKSYLEVVVDVTDTVDDARARARSQLDAIAADLSAALKLG"
     gene            complement(4040..4846)
                     /locus_tag="VGD55_11655"
     CDS             complement(4040..4846)
                     /locus_tag="VGD55_11655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY0871046.1"
                     /translation="MTTAVPSPAESAEQAAARLAEITGVAAHDVAIVLGSGWVPAADA
                     IGTPTHDFAVTDLPGFAPPAVLGHAGRIRSIDAAGRRVLVFLGRTHLYEGRGVDTVVH
                     GVRTAAQAGCRVIVLTNGCGGLREGYVPGQPVLIADHLNLTATSPLHGATFVDLTDLY
                     STRLRSIARDVDPSLTEGVYAQLPGPHYETPAEIRMLRTLGADLVGMSTTLEAIAARE
                     AGAEVLGISLVTNLAAGMTGEPLDHQEVLAAGQASAARMGALLAQVVARL"
     gene            complement(4843..5313)
                     /locus_tag="VGD55_11660"
     CDS             complement(4843..5313)
                     /locus_tag="VGD55_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY0871047.1"
                     /translation="MALYAAYASNLDPEQMLTRAPHSPAQGTGWLQGWRLTFGGEELS
                     FEGALATVVEDAGSQVFVMLYDVPAFDEPELDRWEGNALGLYDKIRVRVATLEGDLTA
                     WLYVLNGYEGGLPSARYLGMIADAAEVAGAPDDYVKDLRLRPCASVDDDLPGAP"
     gene            5351..6772
                     /locus_tag="VGD55_11665"
     CDS             5351..6772
                     /locus_tag="VGD55_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEY0871048.1"
                     /translation="MTRIAIIGGGPGGYEAALVAAQLGAEVTVVERTGVGGACVLTDC
                     VPSKTLIATAEAMTALALSPRLGVRFDGGTDHDAGNITVDATQVNARVRSLAKAQSDD
                     IASRLAAENVRVIAGSARLGAPTVAGHRLTVTSAEGTVETTEQLDADVVLLATGAHPR
                     VLPTAVPDGERILTWHQIYDLDAFPERLIVVGSGVTGAEFAGAYQALGSQVTLVSSRE
                     RVLPGEDADAAAVVEAVFRRRGMTVMSQARASAVTRTADGVVVQLADGSVVEGTHCLM
                     AVGSVPNTDDLGLDDAGVRISASGYIEVDRVSRTSSPGIYAAGDCTGVLMLASVAAMQ
                     GRIAMWHALGEAVRPLRLSMVAANVFTDPEIAAVGVSQSAIDSGGLEVRSVKLPLARN
                     ARAKMAGVTDGFVKLFARPGTGVVLGGVVVAPKASELILPVTMGVQLGLTVDQIAQTF
                     SVYPSLSGSITEAGRQLMETHLD"
BASE COUNT         1202 a         2300 c         2286 g         1114 t
ORIGIN      
        1 ggttcgagga atcggcgccg gcgttgcgcg cggtcagcga gtggctgacc ggaatgggcg
       61 gccgcatcga cacgatcacc gatcaactgc gcgaggtcgg cgacgccgcg tcgcggatcg
      121 gtcgcatcgc cacgcagacg cacatcctgg cgctgaacgc gagcatcgag gccaccaaga
      181 acgtcaccga cggcaggggc ttcgccgtca tcgccgaaag cgtgcgaact ctcgcttcgg
      241 agagtgcggc ttctgcggtg tcgatcaaca cgacgcttgg cgcgctcgcg acctcactcg
      301 acgtattccg ctccgacagc gcggaggcgg acgcccacgc ccggtcggtg caagcaagca
      361 ctgacgccat gtcggagttc atctccgagc tcaccggacg cgccgcgaga gtgtccgact
      421 cggcccagac gatcgaggac accgcaacag ccagccagga agggacgtcg catttcgtcg
      481 actcgctgag ccggctcact gagggcatcg gtcgttcgca gaaggagatc ggccgcagcc
      541 gcgaccgagt cgcgtcgttg ctcgggtcgg cgaacgtggt gctccaggac ctttcggcct
      601 ccggggtgcg gaccgtcgac accccataca tcgatgccgc ggtcaacgcg gccgctcgag
      661 caaaggcgat attcgaggac gccgtggcgc gaggcgcgat cgcacgtgaa gacctgttcg
      721 acgagaacta cgtcccgatc ccaggtaccg atccacttca gttattgacg aagttcaccg
      781 gattcgccga gaagagcgtc ttgccgttga tggaagaagt gcacgcgagc ctgcccgcca
      841 gcgtctattg cgtggcaggt gatcgcaacg gctacgcccc tgcgaacact aaggagtact
      901 cacatccgca aggccccgac cctgcgtgga acgaatcgca ctgccgaaat cgccgaatct
      961 tcgacaacga cgtcgcgacg cgggcggcca agaacacggc gggcccactg atccaggtgt
     1021 accgccgcga tctggggggt ggcacgttcg acatcgtgaa ggatgccgcc gcaccgatat
     1081 tcgtcgatgg ccgccattgg ggcgcgttcc ggatcggcta catagcgggc ggcgctgaat
     1141 catcgagcgc ggcgtcatcg agcagcgact gaggcgcacg cattcgccat gcgtcggtaa
     1201 ccagttcggc caagcggtcg acgtcgactt tctcgagcca gagcatgacc agcgggaagc
     1261 cgtcgtaccc cgaggtcgtg aagaagacgt caggttcccc gagcagtagc gcctgcttct
     1321 ccgcctcgtc gccgacgaac aacaccgcga tgtcggtgcg gatccggcgc cgttctcccg
     1381 gccggcgctc gggataggac cacacgaagc ccttgccgcc cacgcgaaag tcgaagccgt
     1441 cgctcttgat ctcttccacg tgaggaaggg ccagcgccag gcgccggaca tcgtcggcgt
     1501 cagccaccgc tggccctgcc cgatcgcagc gtgtacatgg cccaaaacta gtccgagacg
     1561 acgttgtaca ggtcgacctc ggtctcgacc ggcggcgcgg cgaacccacc ctcgatgccc
     1621 tgctgcatgc ggggcatcag cgtgccgtcg cggaagttct cccagctttc cttcgactcg
     1681 tgcacggcca tgatggtcca gccaccctcg gatgcgcccg ccgcgtgaaa gagctggccg
     1741 gcgggcaacc cgtcggccgg gtggacggcg gcgatcgatg cctcgtactg atccttggtg
     1801 cctcccgtga agaagtgcac tactccgaag ggcttgccgt ccatgtctgc tcccttgacc
     1861 gaagagaata tttgacatgc caagtaatac gatcaatcac ttggcatgtc aagtagttcg
     1921 gatacactaa atccgtgacg acggacgacg tagagcggtg gacgcctgca tggctcgcgc
     1981 tcatccggac ccacgcgcgc ctatgggacc aagtcgaggc acaaatgcgc cacgacagcg
     2041 gtctcacgtt gcctcgctac gacgtcctta tgcagctcga ccgttctggc gggcggctgc
     2101 gcctcaccga gctcgcctca agtgtcgtgc tctcgccgtc aggattgtcg aagcttctcg
     2161 accgcatgga ggcgtccgaa ctgctgcggc gcgagccgga tccaaccgac gctcgttcga
     2221 cccttgcccg cctgacacca cggggccggt tactcgttcg aaaggcacgc cagactcatc
     2281 acgcatggct ccaaaaggca tttggtgacg cactcaacga acgcgacgtc gccgacctca
     2341 cccgcatcat gacccggctc gacgctcaga tctcgagcgc gtaggggctt cgactagccg
     2401 agcttcaatg cggcgctcag atccgcggct attgcgtcga gctgcgatct cgcacgcgcc
     2461 cgtgcgtcat cgacggtgtc ggtgacgtcg acgacaacct cgaggtagct cttcagcttg
     2521 ggctcagtac cactgggccg gacgacgact cgcgcgggcc cgatgcccag tgccgaaccg
     2581 tcgagccgga accgcaaccc gtcggtcggc ggcaaaccgt tggctcccac cgccagatcg
     2641 tcgaagccga cgactggcaa cccgcctagc gacgacggcg gatccgcccg cagccgatcg
     2701 accgcagcac cgatctgcga gaggtccgac accctgatcg acacctggtc ggtggcatag
     2761 acgccatacg agcgagcgat gtcgtcgagc cgatccgcaa gcgttgaccc gtcggccttc
     2821 ggcgtcgcgg ccagcaacgc gacaatcagt gctgcgctga tgccgtcctt gtcgcgcgca
     2881 agcgagggcg ctacgcaata tccgagcgcc tcttcgtacc catacacgcg gtcaccgttt
     2941 cgtacgatcc acttgaaccc cgtcaatgtc tcggagtacg ccatgccatg acgcgctgcc
     3001 atctgcgaaa gcagcgagga ggacacgatc gtcgtcgcgt agcgaccaga aattccttgc
     3061 gtcagtagga aatcggccag caacactccg acctcgtcac cgcgcagcgt ccgccagcca
     3121 ccttcaacgc gcggattcgg aatcgcgacc gcgcaccggt cggcgtccgg atcattggca
     3181 agcacgagat cagccgacgt cgagcgcgca agtgctaacg ccaggtcgat cgcgcctggc
     3241 tcttcagggt tcggaaatgc aacggtcggg aaatccgggt cgggcgcgcc ctgctcggcg
     3301 acaaccgaca acgacgtgaa gcccgcgcgc gtgaacgcgg cgagcacgac gtcacgaccg
     3361 acaccatgca gtggcgtgta cgcaatccgc acgtcacgat gcggcagtgc aacgagcaac
     3421 ggcaacgacg tgatgcccgt caggtaggca tgcagcaccg actcgtcgag cacggtccag
     3481 gcgtcagttc gcgggacgtc ggcgagcggg ccgaccgcgt cgatgtgctg tgagatagcg
     3541 gcatcggccg gcggcacgat ctgaccgccg tcaccgaggt agaccttgta gccgttgtcc
     3601 tgcgggggat tgtgcgatgc cgtcaccatg acgcccgccg agacgcccag ataccgaatc
     3661 gcaaacgcga gtactggtgt cggcagtgcg cggggcagca ccgatgctgt gattccggcg
     3721 ccctccatga cagcggcggt gtcacgcgcg aacacgtcgg acttgtgccg cgcgtcgtac
     3781 ccgatcacta ccgaggtacc gcccgtcgca cgcaggtacg cagcgaggcc agctgccgcg
     3841 cggatcacga ctgcacggtt catgcgattt gggccagcgc cgagcgcgcc gcgcaaaccc
     3901 gctgtgccga actgaagccg cgcgctgaac cgatcggcca gcccctcggc gtcccctcct
     3961 gctagaagag ctgaaagctc ggcgcgagtg tcggggtcgg ggtcctcggc cagccactgc
     4021 gcagcggctg cttcgtagtt cacagccgcg ccacgacctg cgctagcagc gcacccatgc
     4081 gcgccgcaga tgcctggccg gcagcaagca cctcttgatg gtcgagcggt tcgccggtca
     4141 tgccggccgc gaggttggtg accaacgaga tgccgagcac ctcggcaccc gcctctcgcg
     4201 cggcgatcgc ttcgagcgtg gtcgacattc cgacgaggtc agcgccgagc gttcgcaaca
     4261 tgcggatctc tgcgggcgtt tcgtagtgcg gcccgggaag ttgcgcgtag acaccttcgg
     4321 tgagactcgg atcgacgtcg cgcgcgatcg accgcaggcg agtgctgtac agatcggtca
     4381 ggtcgacgaa cgtcgcgccg tgcagcggcg aggtggcggt caggttcagg tggtcggcga
     4441 tgagcaccgg ctggcccggc acgtagccct cgcgaagccc accgcaaccg ttcgtcaaca
     4501 cgatgacccg acagccggct tgcgccgccg tgcggacgcc gtgcacaacc gtgtcgactc
     4561 cgcgcccctc gtacagatga gtccgaccca agaacaccag cactcgccgg ccagccgcgt
     4621 cgatcgagcg aatacgtccg gcgtgcccga gcactgcggg aggagcgaaa ccgggcaggt
     4681 cggtgacggc aaagtcgtgc gtcggcgtgc cgatcgcgtc ggctgccggt acccagccag
     4741 aaccgagcac gatcgcgacg tcgtgcgcgg caacaccggt gatctcggcc agcctcgcag
     4801 cggcctgttc ggccgattcg gcaggggacg ggacagcggt tgtcatggcg ctccaggaag
     4861 gtcgtcgtcg accgaggcgc aggggcgcag gcgcaggtct ttgacgtagt cgtccggagc
     4921 gccggcaacc tcggcagcgt cggcgatcat gccgaggtag cgcgccgacg ggagcccgcc
     4981 ctcgtagcca ttgagcacgt acagccacgc cgtgaggtcg ccctcaagcg tcgcgacccg
     5041 cacccggatc ttgtcgtaca ggccgagggc attgccttcc caccggtcga gttcgggctc
     5101 gtcgaatgcg gggacgtcgt acagcatcac gaatacctgg ctgcctgcgt cttcgacgac
     5161 cgtggcgaga gctccttcga agctcagttc ttcgccaccg aacgtgaggc gccagccttg
     5221 aagccagccg gttccttgcg cgggggagtg tggcgcgcgc gtcagcatct gctcgggatc
     5281 gaggttgctg gcgtaggcag cgtaaagggc cacgcaccga aggttagacg gttacgacac
     5341 aatgcggcat gtgacgcgca tcgccatcat cggaggcgga cccggcggct acgaagccgc
     5401 actcgtcgcg gcacaactgg gcgccgaggt caccgtcgtc gaacgcaccg gtgtgggcgg
     5461 cgcctgtgtg ctcaccgact gcgtgccctc gaagaccctg atcgccaccg ccgaggcgat
     5521 gaccgccctc gcgctgtcgc cgcgcctcgg cgtcaggttt gacgggggca ccgaccacga
     5581 cgcgggcaac atcacggtcg acgccacgca ggtcaacgcc cgggttcgca gcctggccaa
     5641 ggcgcagtcc gacgacatcg ccagcaggct cgccgccgag aacgtgcgcg tgatcgccgg
     5701 atcggcgcgg ctgggcgcac cgacggtcgc gggtcaccga ctcacggtga cgtctgctga
     5761 gggcactgtc gaaacaaccg aacaacttga tgcggacgtc gtgttgctgg cgacgggtgc
     5821 gcacccgcgg gtcctcccga cggctgtgcc cgacggcgag cggatcctga cgtggcacca
     5881 gatctatgac ctcgacgcgt tccccgaacg gctgatcgtc gtcggttcag gcgtcacggg
     5941 cgcggagttc gccggcgcgt atcaggctct gggctcgcag gtcaccctgg tgtcgtcgcg
     6001 cgagcgggtg ctaccgggcg aagacgcaga tgctgcggcc gtcgtcgaag cagtgtttcg
     6061 ccgtcgaggg atgacggtga tgagccaggc gcgtgcatcg gcggtcaccc gcactgctga
     6121 cggggtggtc gttcaactcg ccgacgggag tgtcgtcgag ggaactcact gcttgatggc
     6181 ggtcgggtct gtccccaaca ctgacgatct cggcctcgac gatgcgggcg tgcgcatctc
     6241 ggcgtccggc tatatcgaag ttgaccgcgt ctcgcgcacc tcgtcgcccg gcatctacgc
     6301 agcgggcgat tgcacgggcg tgctgatgtt ggcgtcggtt gccgcgatgc agggccggat
     6361 cgcgatgtgg catgcgctcg gtgaggcggt gcgaccgctg cgcttgtcga tggtcgcggc
     6421 caacgtgttc acggatcccg aaatcgccgc ggttggcgtg tcacaaagcg cgatcgattc
     6481 aggcggcctc gaggtgaggt cggtgaagct cccgctcgca cgcaacgcgc gggccaagat
     6541 ggcgggcgtc accgacggct ttgtgaagct gttcgcccgg ccgggcaccg gggtcgtgct
     6601 cggtggcgtc gtcgtcgcgc caaaggcgag cgagctgatt ctcccggtca cgatgggggt
     6661 gcagctcggg ctcaccgtcg atcagattgc gcagacgttc tcggtctatc cgtcactgtc
     6721 gggcagcatc accgaggccg gccggcagct gatggagacc cacctcgact agcgctactt
     6781 gctgggtgtt gcgctcggct tcttggtcgg cttcggcttt gccgtcgagc tcacgtgcgg
     6841 cttcttcgtg gggctcggcg tcggctccgg aggcggtggc gtcgtcgcct tgggcgagac
     6901 ga
//
LOCUS       DASWVF010000661         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_273631, whole genome shotgun sequence.
ACCESSION   DASWVF010000661 DASWVF010000000
VERSION     DASWVF010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273631"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VGD55_11670"
     CDS             complement(<1..1383)
                     /locus_tag="VGD55_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY0871049.1"
                     /translation="MSPARSASASASRPASRKQVQQLDRVIIRLAGDSGDGMQLTGDR
                     FTSETAGFGNDLSTLPNFPAEIRAPAGTLPGVSSFQLHFADHDIMTPGDAPNVLVAMN
                     PAALKANIADLPLGADIIANADEFTLRACQRIGYAGNPLEDGTLGEYRVSAVPLTSMT
                     VEALKEIDISKKEAERAKNMFALGLLSWMYNRPTESTLEFLERKFAKTPSVAKANVTA
                     FRAGYNFGETTETFSVSYEIKPAPMRAGTYRNISGNLALSYGLVAASQATGLSLFLGS
                     YPITPASDILHELSKHKAFGVRTFQAEDEIAGVTAALGAAFGGSLGVTTTSGPGVALK
                     SEAIGLGVMLELPLIIVDVQRGGPSTGLPTKTEQADLLQAMFGRNGEAPVPIVAPSTP
                     SDCYATALEAARIATKYRTPVFLLSDGYLANGSEPWLLPDVALLPDLRVTFATSPNHV
                     DAKGEPEFWPY"
BASE COUNT          295 a          498 c          482 g          276 t
ORIGIN      
        1 atatggccag aactccggct cacccttggc gtcgacgtga ttgggcgacg tcgcaaacgt
       61 gacccgcagg tcgggcaaca acgcgacgtc cggcaaaagc cacggctccg aaccgttcgc
      121 caggtaacca tcggaaagca agaacaccgg cgtgcggtat ttggtagcga tccgggccgc
      181 ctccagtgcg gtcgcatagc aatccgaggg tgtgctcggc gcgacgatcg gcaccggcgc
      241 ctccccattg cggccgaaca tcgcctgcaa aaggtcggcc tgctcggtct tggtcggcag
      301 cccggtcgac gggccgccgc gctgaacgtc gacgatgatc agcggcagtt cgagcatcac
      361 gccgagcccg atcgcttcgc tctttagcgc aacgccgggc ccagacgttg tggtgacgcc
      421 gagactcccg ccgaacgctg caccaagcgc tgcggtgacg ccggcgatct cgtcctcggc
      481 ctgaaacgtg cgaacgccga aagccttgtg cttggacagt tcatgcaaga tgtcggacgc
      541 cggagtgatc gggtaggagc caaggaacaa cgacaaaccc gtcgcctgcg acgctgctac
      601 caacccgtac gacaacgcca agttgccgga gatattgcgg taggtaccgg ccctcatcgg
      661 cgctggcttg atctcataag agaccgagaa ggtctccgtc gtctcaccga aattgtaacc
      721 agcccggaac gccgtcacgt tggccttcgc gaccgaaggt gtcttggcga acttgcgctc
      781 cagaaactcc agcgtcgatt ccgtcgggcg gttatacatc cacgacagca ggcctagcgc
      841 gaacatgttc ttcgcccgct cggcctcctt cttggagata tcgatctcct tcagcgcctc
      901 aaccgtcatt gacgtcaaag gcactgcaga cacccgatat tcgccgagcg tgccgtcttc
      961 cagcgggttg ccggcgtagc cgatgcgctg gcaggcacgc agcgtgaact catccgcatt
     1021 cgcgatgatg tcagcaccca gcggcaggtc ggcgatgttt gccttcaatg ccgccgggtt
     1081 catcgcgacc aacacatttg gcgcatcgcc gggcgtcatg atgtcgtggt cggcaaagtg
     1141 caactggaac gacgacaccc ccggcagtgt gccggccgga gcccggatct cggcagggaa
     1201 gtttggcaac gtcgacaggt cgttgccaaa accggcggtc tcagaagtga accggtcacc
     1261 cgtgagctgc atgccgtcac cggagtcacc ggcgagccgg ataatcaccc ggtcgagttg
     1321 ctggacttgc ttgcgagagg ctggccgaga cgccgaagcc gacgctgatc gtgcgggtga
     1381 caccgggcgt cgaacctcct cgagggcgtc gactcactgt ggaacaccgc agaaatcgtg
     1441 tgggaacgct gtcgacgcgc ggcttacatg ctacgtcggt tcgcggccgc cctcatggtc
     1501 aggtaccgcg gcgcgcgggt tgatctgggc gatcttggct tcctcttcgt c
//
LOCUS       DASWVF010000662         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_43959, whole genome shotgun sequence.
ACCESSION   DASWVF010000662 DASWVF010000000
VERSION     DASWVF010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43959"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1200)
                     /locus_tag="VGD55_11675"
     CDS             complement(139..1200)
                     /locus_tag="VGD55_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0871050.1"
                     /translation="MRPLNRVLVTIAVSSLVLVAGCSSKKSTAAATSSSPAATAASGS
                     AGGGGDVTLNLVAYSTPQPAYAKLIAAFQKTTAGAHVKFTQSYGASGDQANAVVSGQA
                     ADVVEFSLLTDMAKLVKANIVAPDWNANAQKGFVTDSVVSLVVRKGNPKGIKDWSDLI
                     KPGVKVLTPNPFSSGSARWNILGAYGAQIKEGKTDAEATDYLKSLFKNVLVQDASGSK
                     ALADFVGGAGDVLISYENEAIYAQAKGQAVDYVIPSDTLLIENPLAVTTTTKHPVEAK
                     AFVDFLYSQAGQQIFADIGYRPTLPGVTSTLNKFPTPPGLFTIADLGGWPAATTKFFD
                     PTTGLITAIEKSNGVATTK"
     gene            complement(1498..2715)
                     /locus_tag="VGD55_11680"
     CDS             complement(1498..2715)
                     /locus_tag="VGD55_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0871051.1"
                     /translation="MPRSMSRRQFLITSGVVSGAALAAGATGCSFHHLVAAGKKTPLA
                     VGTKILVLVTMYGGNDGLNTVIPYTDKAYYAGRPDLAYEQADVLPLADDLGLNPSMVG
                     FKKLYDAGQLAVVRGVGYPNADRSHFRSMSIWQTASPDTPQVTGWLGRWLDANGADPL
                     LAVNVGPVLPPLLAGQKCAGAALPSSGGVTLPDGSYGSGLRALDAVSAASQPLMQRVA
                     QSGTDLFRVATTFGPTLASPAAAPKAGGANDIAGQLDLVARCIAANAPTRVYSVQLGG
                     FDTHADEKGTQSQLLGQLDAAVSGFLTKVAAGPHGNDVVVTLYSEFGRRVAANGSQGT
                     DHGTASNVFVAGAGVKGGMFGDQPSLTDLSMDDLKYNIDFRSVYATLLEGVLAADSGE
                     VLGSGSFPRLAFI"
     gene            complement(2727..4034)
                     /locus_tag="VGD55_11685"
     CDS             complement(2727..4034)
                     /locus_tag="VGD55_11685"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0871052.1"
                     /translation="MSTQSSDVLRSDLAHLYRRAGFGATAAELDLAVTAGYAATVAAL
                     VVPQPIADSGVQATPAPQFDVLAPPGKSATAAARQQYATQARSQSLDLALWWVDRMVV
                     ATNPFPEKLTFFWHGHFATAIQKVRSAALMLRQNELFRSQGLGAFDALDLAVARDPAM
                     MIWLDTAQDVASHPNENFARENMELFTLGYGNYTEDDVREAARCYTGWRYNRTTDAFT
                     EVARLHDNGSKTVLGQTGDFDGTDVVRILTHSPASQRWICTRIWCRFARTITTDPVVN
                     ALITTYAQHLDVGELMKAAFTTPAFLTTKGELVKQPVEYVVGALRALKLRADNKKYLS
                     VLQNLGQVPFDPPNVGGWPFDDAWLTTAASYTRLQFARTIASTGDLSAVADESPSTRA
                     DAAAHLLSVDGWGSQTRVALAQVADDPISLVTLALCAPEYVVN"
     gene            complement(4092..>4363)
                     /locus_tag="VGD55_11690"
     CDS             complement(4092..>4363)
                     /locus_tag="VGD55_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY0871053.1"
                     /translation="TKAMRNVAVFVEDTSGDPRLLGLYVGIPLTERTTSYAGVLPDRI
                     MIYRLTICSYCDTEEQVVAEVRKTVVHEVAHHFGIDDDRLHELGY"
BASE COUNT          797 a         1351 c         1415 g          800 t
ORIGIN      
        1 acgaaaccga ggccgagtgc ggtgccgccg cgggagttgc tccccgatcg tgaacgccac
       61 catcccggtt gggtcgtcgt agtaacgggc gaagcggacc gggcgtcatc gcccggcccg
      121 ctcgcgtctt cgagtgtgtt atttggtagt agcgacaccg tttgacttct caatggccgt
      181 gatcaagccg gtggtcgggt cgaagaactt cgtggtcgcc gccggccaac cgccaaggtc
      241 ggcgatcgtg aagagccccg gcggtgtcgg gaacttgttt aacgtcgacg tcacccccgg
      301 aagggtcggg cggtagccga tgtcggcgaa gatctgctga cctgcttgtg agtaaaggaa
      361 gtccacgaac gccttcgcct ccacggggtg cttggtggtc gtggtgaccg cgagcgggtt
      421 ctcgatgagc aacgtgtcgc ttgggatcac gtagtcgact gcctgaccct tggcctgagc
      481 atagatcgcc tcgttctcat acgagatgag cacgtcgccg gcgccgccca cgaagtcggc
      541 aagtgccttc gagccgctgg cgtcctgcac tagcacgttc ttgaacaggc ttttcaggta
      601 gtcagtcgcc tcggcgtccg tcttgccttc cttgatctgc gccccgtagg caccgaggat
      661 gttccaccgt gcgctacccg acgagaacgg gttcggcgtg agcaccttca cacctggctt
      721 gatgaggtcc gaccagtcct tgatgccctt cgggttcccc ttgcgaacga ccagcgagac
      781 taccgaatcg gtgacaaacc ctttctgcgc attggcgttc cagtcagggg cgacgatgtt
      841 cgccttcacc agcttcgcca tgtcggtcag cagcgagaac tcgacaacgt cggcggcctg
      901 gccgctgacg accgcattcg cctggtcgcc cgatgcccca tacgactggg tgaacttcac
      961 gtgcgcacct gcggtcgtct tctggaacgc agcgatcaac tttgcgtacg caggttgcgg
     1021 ggtcgagtac gccacgaggt tcagggtcac gtcgccgccg ccgcctgctg agccgctcgc
     1081 ggcagtcgcg gcgggagaag acgaagtcgc cgcagcggtc gatttcttgg aactgcagcc
     1141 cgcgaccagc acgaggctgg aaacggcaat ggttactagc actctgttca gcgggcgcac
     1201 ggtagttctc caggtttatc taggaatcgt tatctagtaa taagctatgg aatggctgac
     1261 agtcagccat cgaaggctca agcaaaggat tcggggtgaa cggcggcgcg gtgcccgcct
     1321 tcaatctcct ggcgctgcgg tcagcagggc agacacagtg cggacgtcac gcgcagcaga
     1381 tcgacatgaa gtcgctgcgt gaggaggacg cgagtcatga gaggggaccc tagcacatcc
     1441 gacgtgcgtt ctctgctttg cctgtagaga tgcaaccaag caagccaaga gaggtgctca
     1501 gatgaaggcg aggcgcggaa aactcccgct gccgaggacc tcacccgagt ccgcggccag
     1561 cacgccttcg agcaacgtgg cgtagacgga cctgaaatcg atgttgtact tcagatcgtc
     1621 catgctcagg tcggtcaggc tcggctggtc accgaacatg ccgcctttga cgccggctcc
     1681 ggccacgaac acgttcgacg cggtgccgtg atcggtgccc tggcttccgt tcgcggccac
     1741 ccgtcgaccg aactctgagt acagcgtgac cacgacgtcg ttgccatgag ggcctgcggc
     1801 aaccttggtc aggaagcccg acaccgcggc gtccagctgg ccgagaagtt ggctctgggt
     1861 gcccttctcg tcggcgtgag tgtcaaatcc gcccagctga acgctgtaga cgcgggtcgg
     1921 tgcattcgcg gcaatgcagc gagccaccag gtcgagctgg cccgcgatgt cgttcgcccc
     1981 gccggctttg ggcgcggcag caggcgatgc gagcgtcgga ccgaacgtgg ttgccaccct
     2041 gaacaggtcg gtacccgact gagcgacccg ctgcatcaag ggctggctcg ctgccgacac
     2101 ggcgtcgagc gcgcgcaggc cactgccgta actgccgtcg ggcaacgtca caccaccgct
     2161 ggagggcagc gccgcgcccg cgcacttctg gccggcgagc agcgggggta ggacgggacc
     2221 gacattgacc gcgagtagtg gatctgcgcc gttggcgtcg agccagcgac cgagccagcc
     2281 cgtgacctgc ggggtgtccg gagaggcggt ctgccagatc gacatcgagc gaaagtggct
     2341 gcgatcggcg ttcggatatc caacgccgcg gacgacggca agctgcccgg cgtcgtagag
     2401 cttcttgaac cccaccatcg acggattcag cccgaggtca tcagcgagcg gaagcacgtc
     2461 ggcctgctcg taggcgaggt cgggccgtcc cgcgtagtac gccttgtcgg tgtaggggat
     2521 gacagtgttc agcccgtcgt ttccgccgta catcgtgacg agcaccagga ttttggtacc
     2581 aacggccagc ggcgtcttct tgccagctgc cacgagatgg tggaacgaac agccggtcgc
     2641 tccagcagca agtgcagcgc cgctcaccac accgctcgta atgagaaact gccgtctcga
     2701 catcgacctt ggcatggcca tctcctctag ttcacgacgt attcgggcgc gcacaaggcg
     2761 agggtcacga gagaaatcgg atcgtctgcc acctgggcaa gagcgacgcg ggtctgcgac
     2821 ccccagccgt cgactgagag caggtgggca gccgcgtcgg cccgtgtcga cggcgactcg
     2881 tctgcgactg cggagagatc gcctgtgctc gcgatcgttc gcgcgaactg aagtctcgta
     2941 tagctcgccg ccgtggttag ccaggcgtcg tcaaatggcc agcccccgac gttcggcgga
     3001 tcgaacggaa cttggcccag attttgcagc actgacaggt acttcttgtt gtcggcgcgc
     3061 aacttcagcg cccgcagcgc acccacgacg tactcaaccg gctgcttcac aagctcgccc
     3121 tttgtcgtga gaaaggccgg tgtcgtgaac gcggctttca tcaactcacc gacatcgagg
     3181 tgctgcgcgt acgtcgtgat caacgcattc accacgggat cggtcgtgat cgtccttgcg
     3241 aaccggcacc agatgcgggt gcaaatccag cgctgagatg cggggctgtg ggtgagaatg
     3301 cgcacgacgt cggtgccgtc gaagtcgccc gtctgaccaa gcactgtctt cgacccgttg
     3361 tcgtgcaggc gggcaacttc agtgaaggca tcagtcgttc ggttgtaccg ccagcccgtg
     3421 tagcagcgag cggcctctcg tacgtcgtcc tctgtgtaat tgccgtagcc gagagtgaag
     3481 agctccatgt tctcgcgcgc gaaattctcg ttcgggtgcg aagcgacgtc ttgcgccgtg
     3541 tcgagccaaa tcatcattgc gggatcgcgc gccaccgcta ggtcgagcgc atcgaatgca
     3601 ccgagccctt gcgacctgaa tagctcgttc tgccgcaaca tcaacgcggc actgcgcacc
     3661 ttctgaattg ctgtggcgaa gtggccgtgc cagaagaacg tcaacttctc cggaaacgga
     3721 ttggtggcca ccaccatgcg gtcgacccac cacagcgcaa ggtcgagtga ctgactccgt
     3781 gcctgcgtgg cgtactgctg gcgggcagcc gccgtggcgg acttgccggg cggggcaagg
     3841 acgtcgaatt ggggtgcggg cgtcgcctga acacccgagt cagcgatggg ctgcgggacc
     3901 acgagcgcgg cgacggtggc ggcgtagccc gcagtgaccg caaggtcgag ttccgcggca
     3961 gtcgccccaa agccggcgcg gcggtacaga tgtgcaagat cactgcgcaa cacgtcagaa
     4021 ctctgcgtgc tcataggtgc agtagacgcc gcgaatgttc gaggaacatc aggagcgcat
     4081 cacggttgcg ttcaatagcc cagttcgtgc aggcggtcgt catcgatccc aaagtggtgg
     4141 gcgacctcgt ggaccaccgt tttgcgcacc tcggcgacga cctgttcctc ggtatcgcag
     4201 taggagcaga tggtcaatcg gtagatcatg atccggtcgg gcaggacccc tgcgtaggag
     4261 gtcgtgcgct ccgtcagcgg aatgccgacg tagaggccga gcaacctcgg gtcgcctgac
     4321 gtgtcctcga cgaagacggc cacattacgc atcgccttgg tga
//
LOCUS       DASWVF010000663         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_197215, whole genome shotgun sequence.
ACCESSION   DASWVF010000663 DASWVF010000000
VERSION     DASWVF010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197215"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VGD55_11695"
     CDS             complement(<1..1106)
                     /locus_tag="VGD55_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0871054.1"
                     /translation="MAESVVPTAGVRRVRITPNGWAALAGAGLMFALAVQTKSVWMQV
                     VGSALLGLLGISALSVVRRRPGLAVSMQAPTEVFVGAPFDIRIAVSNTGRQTTPPLRI
                     TYDVNVNPPPVAPALVYVDPVAVGDTAVLSVTRVPLRRGAAGSSRLVVDMIGSFGFFT
                     CSYAVAGTPAFFAAPLAAAPIDVTRALSSEAGGSGPMGAGLDVRGVREWRPGDAVRHV
                     HWRSTARTGHLSVLEYGEPTIDMFGVLIAGTRADPRFEAELALAASTTWQGLDDGVMV
                     VITVADDAIPNVKLLKSKAADPELLRLSPTGYHKIFAGLRAAVPDAETVDGLLAHVGA
                     GGIVLLATGDGLADGFVSYVEAAASATGVRLIRVA"
     gene            complement(1099..2112)
                     /locus_tag="VGD55_11700"
     CDS             complement(1099..2112)
                     /locus_tag="VGD55_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0871055.1"
                     /translation="MTSGPMLAALPDGTSRALASAVAARLIGNVERVVHGRRAAVELV
                     AAGLLAGGHVLVQDGPGSGKTTLARAFARSVGGSFRRIQGTADLLPGDITGSAMWDPD
                     QHSFVFVPGPVFANVLLVDELNRATPRAQSALLEALDEGAVTVDGVRHALPEPFFVVA
                     TQNPTDQYGTYPLPEGQLDRFSVALTLGENDLATERQVVREQLLRPTVDDLQPVVAPH
                     DLRWVQDEVRRTHVADGVLDFALNVVRATRRHPAVRIGASSRAALSLVRCAQARTLVY
                     DREFVVPDDVKVLAPYVLGHRLVLQRGVVAQGLRGNGFDALGAGSAVVAEVLASVPVP
                     VRG"
BASE COUNT          430 a          762 c          727 g          320 t
ORIGIN      
        1 gcgacgcgta tcaggcgcac gccagtcgcg ctggccgccg cctcaacgta ggacacgaag
       61 ccgtctgcga gtccgtcgcc ggtggcgagc agaacgatgc caccggcccc gacgtgggcg
      121 agcaagccgt cgacggtttc agcatcggga actgcagcgc gcaggcccgc aaatattttg
      181 tggtaccccg tcggcgataa ccgcagcagt tcgggatctg cagccttgct cttgagcaac
      241 ttgacgttcg gaatcgcatc atcagcgacc gtgataacca ccatcacgcc gtcgtccagc
      301 ccttgccatg tggtcgacgc tgctagcgcc agctcggcct cgaaccgcgg atcggcgcgg
      361 gtgcccgcaa taagtacgcc gaacatgtcg atcgtcggtt cgccgtattc gaggaccgac
      421 aggtgaccgg ttctcgccgt cgaacgccaa tgcacgtgac gcaccgcgtc cccaggtcgc
      481 cattcacgca ctccacgcac gtcgaggcct gcgcccatcg gaccggaacc accggcctct
      541 gaacttaatg cccgtgtgac gtcgatgggc gcagctgcca gcggagccgc gaagaaggca
      601 ggggtgccgg ccactgcgta ggagcaggtg aagaagccga acgacccgat catgtcgacc
      661 acgaggcgac ttgatccggc cgccccgcgc cgaagcggaa ctcgcgtcac cgagagaaca
      721 gccgtgtcac ccaccgcgac ggggtcgacg tagacgagcg cgggcgccac cggcggcggg
      781 ttgacgttca cgtcgtacgt gatacgcaac ggtggagtcg tttgccggcc cgtgttgctc
      841 acggcgatcc ggatgtcgaa cggcgccccg acgaacacct cggtcggtgc ctgcatgctg
      901 accgccagcc caggtcgcct gcgcacgacc gaaagcgccg agataccgag caggcccagc
      961 agcgcgctgc cgacgacctg catccacacg ctctttgtct gcacggctag cgcaaacatg
     1021 aggccggcgc cagccaacgc ggcccacccg ttcggggtga tccgcactcg ccggacgccg
     1081 gcggttggaa cgaccgactc agccacggac cgggaccggc accgaggcca acacctcggc
     1141 gaccaccgcg ctcccggcgc cgagcgcatc gaaaccgttg ccacgcagtc cctgggcaac
     1201 gacgcctcgc tgcaacacga gacgatgccc gagcacataa ggcgcgagga ccttcacgtc
     1261 gtccggtacg acgaactcac ggtcatagac aagcgtgcgc gcttgcgcac atcgcaccaa
     1321 cgacaacgcc gctctcgagc tggcgccgat gcgaacggcg ggatgccgac gcgttgcgcg
     1381 tacgacattc aaagcgaaat cgaggacgcc gtcggcgacg tgggtgcggc gcacttcgtc
     1441 ctgcacccat cgcaggtcat gcggtgccac cacgggctga aggtcgtcga ctgtcggccg
     1501 caacaactgc tcgcgaacga cctgtcgctc ggtcgcgagg tcgttctccc ccaacgtcaa
     1561 agctaccgag aatcggtcga gctgcccttc gggcaacggg taagtgccgt actgatcagt
     1621 ggggttctgc gtcgccacga cgaagaacgg ttcaggcaat gcatggcgga cgccgtcgac
     1681 agtgaccgca ccttcgtcga gcgcttcgag caatgcagac tgcgcgcgcg gagtcgcgcg
     1741 gttcaactcg tcaacgagca gcacgttggc gaacacgggg cctggtacga agacgaaact
     1801 gtgctggtcg ggatcccaca tcgccgagcc ggtgatgtcg ccgggcagca ggtcggcagt
     1861 gccctggatc cgccggaagc tgccaccgac tgaacgtgcg aacgcgcgcg cgagggtcgt
     1921 cttgccgctg ccaggaccgt cctggacgag gacgtgccca ccggcgagaa ggccggcggc
     1981 aaccaactca accgcagcac ggcggccgtg tacgactcgc tcgacgttgc cgatcaggcg
     2041 tgcggccact gccgacgcaa gcgcgcgcga ggtcccgtcg ggcagggcag ccagcatggg
     2101 gccgctcgtc ataggtcctc cgggatcaac gggtgcgctc acttgtctca tcggccgact
     2161 ggcgaccgtc atgaaggatc accggggcag cggatcagac gagaactagt gaacccagta
     2221 gaggaaggcg atctgggtc
//
LOCUS       DASWVF010000664         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401142, whole genome shotgun sequence.
ACCESSION   DASWVF010000664 DASWVF010000000
VERSION     DASWVF010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401142"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /gene="glmS"
                     /locus_tag="VGD55_11705"
     CDS             complement(<1..1556)
                     /gene="glmS"
                     /locus_tag="VGD55_11705"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0871056.1"
                     /translation="MCGIVAYVGPQPARDVVLDGLRRLEYRGYDSAGIAVLADGAVSA
                     RRKAGPLSNLEKMLESDPLPDGGTGLGHTRWATHGRPTDENAHPQLGCRGDVALIHNG
                     VIENHAVLRARLIGDGHVFGSQTDTEVAAHLLEDAFAKYGDLAEAMRQVCHVLEGSFS
                     LVALHREAPGLVVGARRNSPLVVGVGDSEMFLASDVAAFIAHTRDAVELGQDQVVAVT
                     ADGFTITTFDGAAVDGRHYHVDWDVSAAEKGGYDYFMLKEIAEQPDAVAATLLGRLTT
                     DGALMLDEMRLDETLLREVDKIVVVACGSAYHAGMIAKYAVEHWVGLPCEVELASEFR
                     YRDPILDSHTLVIAISQSGETLDTLMAVQHAREMNARVLAICNTVGSTIPREADAVLY
                     THAGPEIGVAATKSFLTQLVAVYLVALYLAQVRGTQKPDDISQVVHELVEVPERLAQV
                     LRTMEPVRSIARSLVGVQTVLFLGRHVGYPVALEGALKLKELAYMHAEGFAAGELKHG
                     PIALVDEGLPV"
     gene            complement(1560..>2239)
                     /locus_tag="VGD55_11710"
     CDS             complement(1560..>2239)
                     /locus_tag="VGD55_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0871057.1"
                     /translation="DLLIATVLATGADVGVAHDGDADRCLAVGADGSVVDGDQILAIL
                     AIAMHESKRLVNDTVVATVMSNLGFRLSMEAAGISIVETAVGDRYVLEAMRAGGHNLG
                     GEQSGHVILLDYANTGDGILTALQLLSQVAHTKQPLVELAAVMHRLPQVLINVTDVDR
                     SRAETDAELLAAIARAQGDLGASGRILLRPSGTEPVVRVMVEATTRAKAEAVAGELAG
                     VVRERLG"
BASE COUNT          404 a          754 c          703 g          378 t
ORIGIN      
        1 acgggcaggc cctcgtcgac cagtgcgatg ggtccgtgct tcaactcacc agccgcaaac
       61 ccctcggcgt gcatgtaggc gagttccttc agcttcagcg cgccttcgag cgcgaccggg
      121 tagccaacgt gacgccccaa gaacaacacg gtctggacgc cgaccagtga ccgtgcaatc
      181 gaacgcaccg gctccattgt cctgaggacc tgcgccaacc gctctggcac ctcgaccagc
      241 tcatgcacga cctgtgaaat gtcatcgggc ttctgggtgc cacgcacttg cgcgagatag
      301 agagcgacca ggtagacggc gacaagctga gtgagaaagc tcttcgttgc ggcgacaccg
      361 atctctgggc cggcatgggt gtacagcacg gcgtcggctt cacgcggaat cgtcgaaccg
      421 acggtgttgc agatcgcgag tactcgggcg ttcatctcgc gtgcgtgctg cactgccatc
      481 aacgtgtcga gggtctcacc cgactgcgag atcgcgatga ccaacgtgtg gctgtcgagg
      541 atcgggtcgc gatagcggaa ttcactcgcg agttcgactt cgcacggcag gccgacccag
      601 tgctccacgg cgtacttggc gatcatgccg gcgtggtagg cagatccaca cgcgaccacc
      661 acgatcttgt cgacctcgcg cagcaacgtc tcgtcgagtc gcatctcgtc aagcatcaac
      721 gcgccgtcgg ttgtgagccg accgagcaac gtcgccgcga ccgcgtctgg ttgctcagcg
      781 atctccttca gcatgaagta gtcgtagccg cccttttcgg cggccgagac gtcccagtcg
      841 acgtggtagt gccgaccgtc gacggcggcg ccatcgaagg tcgtgatcgt gaatccgtca
      901 gccgtcaccg caacgacctg gtcttgaccg agctcgaccg catcgcgggt atgcgcaatg
      961 aaagccgcga catcgctggc caggaacatc tccgagtcac cgacccccac gacgagcggc
     1021 gagttacgcc gcgcaccgac gacgaggccc ggtgcttcgc ggtgcagggc gacgagactg
     1081 aacgagccct cgagcacgtg acacacctgg cgcatcgctt ctgcaagatc gccgtacttt
     1141 gcgaacgcgt cttcaagcaa atgcgccgcg acctcggtgt cggtctgcga tccgaacaca
     1201 tgaccgtcgc cgatcaggcg cgcgcgcaag actgcgtggt tttcgatcac gccgttgtgg
     1261 atcagagcga cgtcaccgcg gcaacccaat tgcgggtgcg cgttctcatc cgtgggtcgg
     1321 ccgtgcgtgg cccacctggt gtgtccgaga ccggttccgc catcgggcaa cggatccgac
     1381 tcgagcatct tttccagatt cgagagtggg ccggctttgc gtcgcgccga caccgcgccg
     1441 tccgctagaa ccgcgatgcc agccgagtcg tagccgcggt attccaaccg ccgcaacccg
     1501 tcgagcacga cgtcacgagc gggctgcggg ccgacgtagg cgacgatgcc gcacacggtc
     1561 tagcccaacc gctcgcgcac gactccggca agctcaccgg cgacggcctc ggccttggcg
     1621 cgagtggtgg cctcgaccat gacccgaacg accggctcgg tgccgctcgg acgcagcagg
     1681 atccgacccg atgcaccaag gtcaccctga gcgcgcgcga tagccgcaag gagttcggcg
     1741 tcggtctccg cacgcgagcg gtcgacgtcg gtgacgttga tcagcacttg cggcaaccgg
     1801 tgcatgacgg ctgccagctc gacgagtggt tgcttcgtgt gcgctacctg cgagagcaat
     1861 tgcaacgcag tcaggattcc gtcgccggtg ttcgcgtagt cgagcaggat cacgtgcccg
     1921 ctctgctccc cgccgaggtt gtggccacca gcccgcatcg cctcgagcac gtagcggtca
     1981 ccgacggccg tctcgacgat ggagattccc gccgcctcca tgctgagccg gaagccgaga
     2041 ttcgacatca cggtggcaac gaccgtgtcg ttgaccagac gtttcgattc gtgcatcgcg
     2101 atcgccagga tcgccaggat ctggtcgccg tcgacgaccg acccgtcagc gccgactgcg
     2161 aggcaccggt cggcatcgcc atcgtgcgcg acgccgacgt cggctccagt cgcgaggacc
     2221 gtcgcaatca ggagatcga
//
LOCUS       DASWVF010000665         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_324229, whole genome shotgun sequence.
ACCESSION   DASWVF010000665 DASWVF010000000
VERSION     DASWVF010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324229"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..698
                     /locus_tag="VGD55_11715"
     CDS             144..698
                     /locus_tag="VGD55_11715"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0871058.1"
                     /translation="MSDDMFEDVLRAAKAGDENAFAQIWRALNPPVERYLRTLAPSSA
                     GDLASETWLQVIRNLSSFSGGEPAFRAWVFTIARNKVIDAARHDKRRPLTTSDNSAID
                     ACASPDDTALLAMERLSTDSALALIATLPSDQAEVIMLRVVAGLDAATVATIVGKSPG
                     AVRVAAHRGLRRLQELVGRPPVTL"
     gene            757..>1701
                     /locus_tag="VGD55_11720"
     CDS             757..>1701
                     /locus_tag="VGD55_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871059.1"
                     /translation="MPNLPFRRRHGDPADAPQLDELLDAGRLDASAAPEWQSVSDVLR
                     SAAAAAEPSELAGEAAIMAAFRRERVGVRHRRPQPIARCKSMFSTLLTGRIAVGLAAG
                     VVTVTGAATAAYACVLPSPIQSFAHSTIGAPKPSVSKVAAVTAVDAANWKHDNDPSVT
                     PSVTVSSSVTVSPSTSTSPKPHPSFSLDPTQAAAVQALIIYRVCQEYAVVTKDGKTLD
                     PKALALLTKAAGSASAIAAYCAALPVPPKFCTATPLPTVTATATPTASPSPKTDNGKG
                     DWWRWCGICPAVTATPSVSPTATATTDADGDHDGSGKFK"
BASE COUNT          290 a          604 c          523 g          284 t
ORIGIN      
        1 ctccgggtct tttctggtcc tcgtccgagc gtacgcggag tgatcatcga cgccatcacg
       61 tccgcacctg acatctgccg gaggtttggc gtctggggat tcttcgttag ggtcgacccc
      121 gagggtgccg atgacatgcg gggatgagcg acgacatgtt cgaggacgtt ctccgcgccg
      181 ccaaagctgg cgacgagaat gcgttcgccc agatctggcg cgcgctgaac cctcccgtcg
      241 agcgctattt gcgcaccctt gcaccgtcgt ccgcgggcga tctcgcctcg gagacctggc
      301 tccaggtgat ccgcaacctg tcctcgtttt caggcggaga gcccgccttt cgcgcctggg
      361 ttttcacgat cgcgcgtaac aaggtcatcg acgccgcccg ccatgacaaa cggcgtcccc
      421 tcacaacgtc agacaactcg gcgatcgacg cgtgcgcctc gccagacgac accgcactcc
      481 tcgcgatgga gcgcctgagc acggattccg cgctcgctct catcgcgacg ctcccgagcg
      541 accaggccga agtcatcatg ctgcgggtgg tcgcagggct tgatgcggcg acagtcgcca
      601 ccatcgtggg caagagccca ggcgcagtgc gggtagccgc ccaccgcggg ctgcgtcgcc
      661 ttcaggagct cgtgggccgc cccccagtca cactgtgagc tcgccaagaa ggagcccagt
      721 aacgctatga acgacgatga cgtttgggga ggtgagatgc caaacctgcc tttccggcga
      781 cgccacggcg acccggccga cgccccgcag ctcgatgagc tgctcgatgc cggccgtctc
      841 gacgcaagcg ccgcccccga atggcagtcg gtgtcggatg tgctgcggtc cgcggctgca
      901 gctgctgagc cttccgaact tgctggcgag gcggccatca tggccgcctt ccggcgggaa
      961 cgtgtgggcg tccgacaccg tcgaccccaa ccaatcgcga ggtgcaagag catgttctcg
     1021 actctgctca ccggacggat cgccgtcggc ctagcggccg gcgtcgtcac tgtcaccggc
     1081 gcagccacgg ccgcgtacgc gtgcgtcctg cccagcccga ttcagtcgtt cgcccacagc
     1141 acgatcggcg caccaaagcc gagtgtttcc aaggtcgcgg cggttactgc tgtcgatgcc
     1201 gctaactgga agcacgacaa cgatcccagc gtgaccccat cagtcacggt cagctcgtcg
     1261 gtcacggtct cgccgtcgac ctcgacgtcg cctaagcccc acccgtcgtt cagtctcgac
     1321 ccgacccaag ccgctgccgt tcaggcgctg atcatttatc gcgtctgcca ggagtacgca
     1381 gtggtgacga aggacggcaa gactctcgac cccaaggcac tcgcgctcct gacgaaggct
     1441 gcgggcagtg cgtcagcaat cgctgcgtac tgtgctgcac tcccggttcc gccgaagttc
     1501 tgcacggcga caccgcttcc gacggtcacc gccaccgcga ctccgaccgc cagcccgagt
     1561 ccgaagacgg acaacggcaa gggcgactgg tggcgctggt gcggaatctg tcccgcagta
     1621 acggccactc cgtcggtctc cccgaccgcc acagcgacaa ccgacgctga cggcgaccac
     1681 gacggctcag gcaagttcaa g
//
LOCUS       DASWVF010000666         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_69312, whole genome shotgun sequence.
ACCESSION   DASWVF010000666 DASWVF010000000
VERSION     DASWVF010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69312"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1360)
                     /locus_tag="VGD55_11725"
     CDS             complement(125..1360)
                     /locus_tag="VGD55_11725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY0871060.1"
                     /translation="MTSDATLHGPWTQLPPARSAPIRAAIARRIFVRVAQQVGVRVEL
                     PKGQALVKGAATAPGIRLYRPAQFFRRVGRDGLIGFGESYMAGDWDADEGSDLASVLT
                     PLARRMATLVPGVLQRVRRAYDARQPASEKNDLRGAKQNIARHYDLSNELFALFLDDT
                     MTYSSALWDEQSPDGPLSAAQVRKIDRLLDLAEVRAGTRLLEIGTGWGELAVRAACRG
                     AHVTTLTLSREQQALAVRKAIARGVAELVDIQLLDYRQATGTYDAVVSVEMIEAVGAE
                     FWPTYFATIDRVLAPGGRVAIQAITIDHQRLLATKGSYTWIHKYVFPGGIIPSLHAVR
                     EVVTEHTRLRVTSATAHGLDYARTLHQWLERFHANAAGVHGLGFDERFIRMWRFYLAY
                     CEAGFASGYLDVHQLVLEH"
     gene            complement(1357..2142)
                     /locus_tag="VGD55_11730"
     CDS             complement(1357..2142)
                     /locus_tag="VGD55_11730"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY0871061.1"
                     /translation="MVTRERVLDLRTTHDLAGVVTPALYRTRIGHTRHRPWRHAFRYR
                     HPMWLVDVARLPRGVLLGGLLRFEARDHLGDPTRSIHENVVAFAAEHDVDVSDDRIVM
                     LANARMFGYVFNPITVFWCLTSEGGHEAVRCVIAEVHNTYGGRHCYFLVPDDSGRAST
                     DKALYVSPFNPVDGRYDMRFSKPAERVHVVVALHRAGALAFSASLSGSRAAPTVGAVL
                     RTIAHYPLGALQVSALIRYQGIRLRLRGLPIVDRPTAARGSTR"
     gene            complement(2136..3374)
                     /locus_tag="VGD55_11735"
     CDS             complement(2136..3374)
                     /locus_tag="VGD55_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0871062.1"
                     /translation="MIGAGVSGLTAAWILQRDAEVTLYEALPRLGGHAHTHDVPAGNR
                     FVAVDSGFIVHNERTYPNLLRLFSELGVATQQTQMSMSVRCDGCGLEYAGAKGFGGVF
                     ARAGSLRKPAYLRMLTEVKRFHLAARRLLAHPEEQPQTLKTFLREGGYSRYFVQHFMV
                     PVVSCVWSCSPGTALLYPARYLFQFLDHHGLLSVSGSPTWRTVVGGSRTYVERATKEL
                     AGVRTQTPVRAVIRGVNSVEIRDADGLAHSYDQVVIATHADEALAMIADPTPEQRRIL
                     GAFTYSLNRTVLHTDASLLPASNRVRASWNYRLDACQTTADRVQVSYDMNRLQGLDEP
                     ANYVVTLNADDRIDASRVIAAMDYTHPIYTTESLAAQHELPALSDHLVAFAGAYHGWG
                     FHEDGCASGVRAAESLGSTW"
     gene            complement(3389..>3718)
                     /locus_tag="VGD55_11740"
     CDS             complement(3389..>3718)
                     /locus_tag="VGD55_11740"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY0871063.1"
                     /translation="LDTFRKDPANKGKVLDRGLWRYTRHPNYFGDACLWWGLGLIAFA
                     HWPGIATIASPALMTWFLSRKTGKPLLESRMATTRPGYAEYIERTSGFFPLPPRTLQP
                     TRPGTAV"
BASE COUNT          643 a         1187 c         1277 g          611 t
ORIGIN      
        1 cgtgccgcgc tctgggacga actgaccgcc gagttcgtgt tgcagtcggc ggtcggtgtg
       61 cgaccgggca cctgcctggc cgcggcgatc gaggccggtg actccgtcct ctaactcggc
      121 agcatcagtg ttcgagcacg agttggtgca cgtcgaggta gccgctcgcg aagccggcct
      181 cgcagtaggc gaggtagaac ctccacatcc gaatgaaccg ctcgtcgaac ccaagcccgt
      241 gcaccccggc ggcgttcgca tggaatcgct cgagccactg gtgcagcgtg cgggcgtagt
      301 cgaggccgtg agcagtggct gatgtcacgc gcaggcgggt gtgctcggtg acgacctcgc
      361 gaaccgcgtg aagtgagggg atgattccgc cgggaaacac gtacttgtgg atccaggtat
      421 acgagccctt ggtcgcaagc agtcgctggt ggtcgatcgt gatcgcctgg atcgcgaccc
      481 gaccaccagg cgccagcacc cgatcgatcg tggcgaagta ggtgggccag aactccgcgc
      541 cgaccgcctc gatcatctcg acgctgacga ccgcatcgta ggtcccggtg gcctgccggt
      601 aatcaagcag ttggatgtcg acaagctcgg cgacgccgcg cgcgatcgct ttgcggaccg
      661 caagcgcctg ctgctcgcgc gacagcgtca aggtggtcac gtgtgccccg cggcacgccg
      721 ctcgcaccgc cagctcaccc cagccggtgc cgatttcgag aagccgggtg ccagcccgaa
      781 cctcggcgag atcgagcagc cgatcgatct tgcgcacttg agctgcgctc agtggcccgt
      841 cgggtgactg ctcgtcccac agcgcgcttg agtaggtcat cgtgtcgtcg aggaacagcg
      901 cgaacaattc gttcgacagg tcatagtgcc gcgcgatgtt ctgcttggcg ccgcgcagat
      961 cgttcttctc ggacgccggt tggcgggcgt cgtacgcgcg acgaacgcgc tgaagcacgc
     1021 ccggcaccag cgttgccatg cgtcgcgcta gcggggtcag gaccgaagcg agatcgctgc
     1081 cttcgtcggc gtcccaatca ccggccatgt agctctcacc gaatccgatg agaccgtcac
     1141 gacccacgcg gcgaaagaat tgcgcaggtc ggtacaagcg gatgccaggc gccgtcgctg
     1201 cgcccttcac cagcgcctga cccttcggca attcgacgcg gacgccgacc tgctgcgcca
     1261 ctcgcacgaa gatccggcgt gcgatggccg cgcggatcgg tgcgcttcgc gcgggaggga
     1321 gttgcgtcca cggcccgtgg agcgtggcgt cgctcgtcat cgggtcgacc cccttgcggc
     1381 cgtcggacgg tcgacaatcg gcagcccccg taaccgaagc cggatgccct ggtaacggat
     1441 cagtgcgctc acctgcagcg ccccgagcgg gtagtgcgca atcgtgcgaa gtaccgcgcc
     1501 gacggtggga gcggcgcggc ttccggacag gctcgcgctg aatgcgagcg caccggcccg
     1561 gtgcagtgcg acgacgacat gtacgcgctc ggcgggcttg ctgaaccgca tgtcgtagcg
     1621 gccgtcgacc gggttgaacg gcgagacgta cagggctttg tcggtactcg cgcgtccgga
     1681 gtcgtcagga acgaggaagt agcagtgccg accgccgtac gtgttgtgca cctcagcgat
     1741 cacgcatctc acagcttcgt gcccgccctc tgaggtaagg caccagaaca ccgtgatcgg
     1801 attgaagacg tagccgaaca tccgcgcgtt ggcgagcatc acgatgcggt cgtcggacac
     1861 gtcaacgtcg tgttcagcgg caaatgccac cacgttctcg tgaatcgaac gggtggggtc
     1921 gccgaggtgg tcgcgggcct cgaaccgcag cagccccccg agcagcacgc cgcgcggcag
     1981 ccgcgccaca tcgaccagcc acatggggtg gcggtagcga aaggcgtggc gccacggtcg
     2041 gtgacgcgtg tgcccgatcc tggtgcggta cagcgcgggc gtgaccaccc cggcgagatc
     2101 gtgggtcgtg cgcaggtcga gaacccgctc gcgggtcacc atgtcgaacc cagactctcc
     2161 gcggcgcgga ccccggatgc gcagccgtcc tcgtggaatc cccagccgtg gtaggcgccc
     2221 gcgaaggcta cgaggtggtc gctcaaagcc ggcaactcgt gctgcgcagc tagcgactct
     2281 gtcgtgtaga tcgggtgggt gtagtccatc gcggcgatga cccggctggc gtcgatccgg
     2341 tcgtcggcgt tcagcgtgac gacgtagttc gcaggctcat cgagaccttg cagccggttc
     2401 atgtcgtagc tgacctgtac gcgatcagcg gtcgtctggc aagcgtcgag ccggtaattc
     2461 catgacgcgc ggacgcggtt cgacgccggc agcagcgagg catcggtgtg cagcaccgtg
     2521 cggttcagcg agtaggtgaa cgcgccgagg atcctgcgct gttcgggagt cgggtcggcg
     2581 atcatcgcca gcgcctcgtc ggcatgtgtc gcaatgacca cctgatcgta ggagtgcgcc
     2641 agcccgtcgg cgtcccgtat ctcgacgctg ttgacgccgc gaattacggc gcgtacggga
     2701 gtctgggtgc gaaccccagc gagctctttg gtggcgcgct cgacgtaggt gcgcgaacct
     2761 ccgacgaccg tgcgccaggt cggcgatccc gacaccgaca gcagcccgtg gtgatcaagg
     2821 aactggaaca ggtatcgggc cgggtacagc agagcggtgc ccggtgagca cgaccacacg
     2881 cacgacacaa ccggcaccat gaagtgctgc acgaagtaac gcgagtaacc gccctcgcgc
     2941 agaaacgtct tgagcgtctg gggttgctct tcggggtggg cgagcaatcg ccgtgcagca
     3001 aggtggaacc gcttcacctc ggtgagcatg cgcaggtagg cgggctttcg cagtgaacca
     3061 gcacgcgcga acaccccgcc gaagcctttc gcccccgcgt attcgagtcc acaaccatcg
     3121 cagcgcaccg acatgctcat ctgcgtctgt tgggtcgcca cgccgagttc ggagaacagc
     3181 cgaagcaggt tggggtaggt ccgctcgtta tggacgatga acccgctgtc gacggcaaca
     3241 aaacgatttc cagccgggac gtcgtgtgtg tgggcgtgtc caccaagtct cggtagcgct
     3301 tcgtaaaggg tgacttcagc gtcgcgttgc aggatccagg ctgccgtgag cccggagaca
     3361 ccggcaccga tcaccgcgac ggatcgcctc atactgcggt tcccggccgg gtgggctgga
     3421 gcgtacgggg agggagtggg aagaacccgc tcgtgcgctc gatgtactcc gcgtagccag
     3481 gtcgggtcgt ggccatacgc gactcgagca acggcttgcc ggtcttgcgg gagaggaacc
     3541 aggtcatcaa cgcgggggag gcgatcgtcg caatgcccgg ccaatgggcg aacgcgatga
     3601 gcccgaggcc ccaccacaag caggcgtcac cgaagtagtt ggggtggcgt gtgtagcgcc
     3661 agaggccgcg gtccaggacc ttgcccttgt tggccgggtc cttcctgaac gtgtcgag
//
LOCUS       DASWVF010000667         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_273762, whole genome shotgun sequence.
ACCESSION   DASWVF010000667 DASWVF010000000
VERSION     DASWVF010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273762"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VGD55_11745"
     CDS             <1..1004
                     /locus_tag="VGD55_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871064.1"
                     /translation="TAPAGGWYFCQGDPGAATCADGTGTLVHQLAMPMLGPGDVTYNP
                     NDGNLYYREVLSNAHGNVERISRVALIGGAPQILVQGPDSVNETEVSFGTPVSSPDGN
                     YLAYWQMTLRFNVPGGPTQAPIPGAQTVGPPGDLQNEPTTTRLVQIKIQNLRDLKTAP
                     VIVPLSMVNDSGPLLGWSADSKQLLLVGPGGKVESLNVGSDGVPLGVNGTVFDPALVV
                     PGCQVSQTLMSSSSDFFVVASCAQTIEVVRVQGGAPRPFGMLVNTKGWEVSDAAIDTT
                     GNVLALSWFAPPGPPQCVEVDGSARIIDGVPTAIQLETTPGCMTAGGPAAPPPSSSP"
     gene            1067..1681
                     /locus_tag="VGD55_11750"
     CDS             1067..1681
                     /locus_tag="VGD55_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0871065.1"
                     /translation="MLGTPTRDRQAERREATRAEILDAAWAIAHAKGLTELTLRDVAE
                     AIGMQAPSLYSYFASKNAIYDAMFGQAWTQYLAAMTELEPTLPRSPRAGIKAIAHTFF
                     DFAIAVPARHQLINERVISGFVPSAESYAPAVEVLERTRAHMERYGVTKPDQFDLCIA
                     IIGGLVNAQLANDPGGDRWARLLDQAVDMFADHLDIPRSRRRTR"
BASE COUNT          317 a          580 c          564 g          303 t
ORIGIN      
        1 caacggcgcc ggccggcggc tggtatttct gccagggcga ccccggcgcg gccacgtgcg
       61 ccgatggcac ggggacgctg gtgcatcagc ttgcgatgcc gatgctcggc cccggtgacg
      121 tgacgtacaa cccgaacgac ggcaacctct actaccgcga agtgctcagc aacgcgcacg
      181 gcaacgtcga acgcatcagt cgggttgctc tcatcggtgg cgcgccgcag atcctggtgc
      241 aaggccccga ctcggtgaac gaaaccgagg tgtcgttcgg cacgccggtc tcctcacccg
      301 atggcaacta cctcgcctac tggcagatga cgttgaggtt caacgtgccg ggcggcccga
      361 cgcaagcacc gatccctggc gcgcagactg ttggcccacc cggtgatttg caaaacgaac
      421 cgaccaccac gcgacttgtg caaatcaaga ttcagaacct gcgcgacctc aagaccgcac
      481 ctgtcatcgt tccgctctcg atggtgaacg attccggtcc gctgctcggc tggtcggccg
      541 acagtaagca gctccttctg gtcggccccg gcggcaaggt cgagtcgctc aacgtcggca
      601 gcgacggcgt cccacttggc gtcaacggca cggtgttcga cccggcattg gtggtgcccg
      661 gctgtcaggt ttcgcagacg ttgatgtcgt cgtccagcga tttctttgtc gtcgcgtcat
      721 gcgcgcagac gatcgaggtc gtcagggttc agggtggggc gccgagaccg tttggcatgc
      781 tcgtgaacac caaggggtgg gaggtcagcg atgccgcaat cgacacgacc ggcaacgtgc
      841 tcgctctcag ctggttcgcg ccgcccgggc cgccgcagtg cgtggaggtc gacggcagtg
      901 cgcgcatcat cgacggagtg ccgaccgcga tccagctcga gacaacgccc gggtgcatga
      961 cggccggcgg accggcagcg ccgccgccgt cgtcctcgcc atagacgcag gttgcaaagt
     1021 tgccgaggct tgacgaatca gctcggcctg cgccatgctc ctaatcatgt taggcactcc
     1081 gactcgcgat aggcaggcgg aacggcgtga ggcgactcgc gccgagattc ttgacgcggc
     1141 gtgggcgatc gcacacgcga agggtcttac cgagctgacc ttgcgtgacg tcgccgaggc
     1201 gatcggcatg caagcgccgt cgctctactc gtactttgct tcgaagaacg ccatctatga
     1261 cgcgatgttc ggtcaggcct ggacgcaata cctggccgcg atgaccgagc tcgaaccgac
     1321 tcttccgcga tcgccgcgcg ccggcatcaa ggcgatcgcg cacacgttct tcgactttgc
     1381 gatcgccgtt ccggcgcgtc atcagttgat caacgagcga gttatctcag gcttcgtgcc
     1441 gagtgcggag tcgtatgcac cagccgtcga agtgctcgaa cgcacccgag cccacatgga
     1501 gcggtacggc gtcacgaagc ccgaccagtt cgatctgtgc atcgcgatca tcggcgggct
     1561 ggtcaatgcc cagctggcga acgaccccgg cggcgaccgc tgggcccgac tgctcgacca
     1621 ggcagtcgac atgtttgccg accacctgga catacctcga tcgcgaagga gaacccgatg
     1681 acgatcaccg ccgagtcgac gaaatcgcgg ccacgcacct cgacgctcga tagggcgacg
     1741 ttgatgcagt tggctgccgc cgag
//
LOCUS       DASWVF010000668         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_477625, whole genome shotgun sequence.
ACCESSION   DASWVF010000668 DASWVF010000000
VERSION     DASWVF010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477625"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGD55_11755"
     CDS             <1..343
                     /locus_tag="VGD55_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871066.1"
                     /translation="WWRWFDGAAWTAHTHPMSQPPFGFGPAPQQRDRALEMVLPINRL
                     PLAIVAGYLGIFSLLVFPGPIAVFVSVIAIRQLGSKPDVAGRGRAWFGLIAGALASVI
                     LVSIIANSLNH"
     gene            complement(368..1243)
                     /gene="hisG"
                     /locus_tag="VGD55_11760"
     CDS             complement(368..1243)
                     /gene="hisG"
                     /locus_tag="VGD55_11760"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0871067.1"
                     /translation="MLSVVLPKGSLEASTLDLFEAADLGVSRGSDRDYHASIDDPRID
                     TVRFLRPQEIPTYVEQGLFDLGITGRDWITETDADVVSLGELEYSKATSNPVRIVLAV
                     VKDHIAERGDQLPDGVRVSTEFPQLTKRYFDRIGVRATIVPSYGATEAKVPDIVEAIV
                     DLTETGTSLRKHGLKIIDTLLTSRTEVITNAAAYADPEKRAAMDDVMLLLRGAVRARG
                     NVLIKLNVADAQLPAVLAVLPSMGSPTVTALASGDMHAVESVVPKRGVNMLIPTLKAA
                     GARDILEIAISKIVD"
     gene            complement(1287..1817)
                     /gene="ribH"
                     /locus_tag="VGD55_11765"
     CDS             complement(1287..1817)
                     /gene="ribH"
                     /locus_tag="VGD55_11765"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0871068.1"
                     /translation="MTGTGRPDLATIDASGLSLGIVAARWNTEVVDALVQRALEAARA
                     CGVREPVVVRVAGALELPVVAQQLAVEHDAVVCLGAVIRGGTPHFEYVCDAVTAGLTR
                     IALDAATPIGNGVLTCDNLAQALDRCGLPDSAEDKGWEATVAALDAAQVLRSLRHPRR
                     NVGPGVGPGFGGPASS"
     gene            complement(1831..3141)
                     /locus_tag="VGD55_11770"
     CDS             complement(1831..3141)
                     /locus_tag="VGD55_11770"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY0871069.1"
                     /translation="MTFDAIDRAIEEIAAGRAVIVVDDEDRENEGDLIAAASKVTPEL
                     VAFMVRHTSGVLCVPMQSATLDRLGLPPMTSRNEDAKQTAYTVSVDARRGITTGISAA
                     DRAHTNRVLADSATEPYELTRPGHVFPLRAVAGGVLRRTGHTEAAVDLAVLAGLTPAG
                     VISEIVDDDGSMARLPRLLEFAKEHSLAIVSIADLVAYRRRHETTVKRVAEARLPTSH
                     GEWRAVGYRSTLDDAELVALVMGEVGGHDDDGENVLVRAHSECLTGDVFASLRCDCGP
                     QLDAAMSAIALEGRGVVLYLRGHEGRGIGLMRKLHAYELQDNGADTIDANLELGLPAD
                     ARDYGTGAQVLVDLGVRSMRLLTNNPAKRAGLEGWGLSVVGRVPLPTHHNPDNMRYLL
                     TKRDRMGHDLLLPGHPQFVAVPPVDASVDAVDSVDSVDQGGGLS"
     gene            complement(3138..>3512)
                     /locus_tag="VGD55_11775"
     CDS             complement(3138..>3512)
                     /locus_tag="VGD55_11775"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HEY0871070.1"
                     /translation="ALAALGVAVLLPVLQSIGEGEFFHPSWLYYLADSWIFVGSMVAT
                     YAMARGWTEFWLCWIGVDIVGVPELLHYHYYPSAVLYAIYAGFVIWGFFVWRQLQRTA
                     LVPRGLSPIAVATEHAGQEASG"
BASE COUNT          603 a         1176 c         1140 g          593 t
ORIGIN      
        1 ctggtggcgc tggttcgacg gcgcggcctg gaccgcccat acccatccga tgtcccagcc
       61 gccgttcggt ttcggccctg cgccacagca gcgggaccgc gcactggaga tggttctccc
      121 gatcaaccgg ttgccactcg cgatcgtcgc cggttacctc gggatcttca gcctgctcgt
      181 gttccctgga ccgatcgccg tcttcgtctc cgtgatcgcg atccgacagc tggggtcgaa
      241 gccggacgtt gcaggccgcg ggcgtgcctg gttcgggttg atcgccggtg ccctggcgag
      301 cgtgatcctg gtcagcatca tcgcgaactc gctcaaccac tgaactcgct caagacctga
      361 gcggccgtca gtcgacgatc ttcgagatcg cgatctcgag aatgtcgcgg gcgccggcag
      421 ccttcagggt cgggatcagc atgttgacgc ccctcttggg tacgaccgac tcgactgcgt
      481 gcatgtcgcc cgacgccaac gccgtgacag tcggcgaacc catcgacggc aatacggcaa
      541 gaaccgcagg cagttgggcg tcggccacgt tgagtttgat gagcacgttg ccgcgagccc
      601 tgacggctcc gcgcaacagc aacatcacgt cgtccatcgc ggcgcgtttc tcgggatcgg
      661 cgtaagcagc tgcattcgtg atgacctcgg tgcgcgacgt caacaacgtg tcgatgatct
      721 tcaggccgtg cttgcgcagg cttgtaccgg tctcggtgag atcgacgatt gcctcgacaa
      781 tgtcgggcac cttcgcttcc gtcgcgccat agctcggcac gatcgtcgcg cggacgccga
      841 tccgatcgaa atagcgcttc gtgagctgag ggaactcggt cgagacccgc acgccgtcgg
      901 gtagttgatc cccgcgctcg gcgatgtggt ccttcaccac cgccagcacg atgcgcaccg
      961 ggttgctcgt ggccttggag tactcgagtt cgccgagcga caccacgtcg gcgtcggtct
     1021 cggtgatcca gtctcgacca gtgatgccga ggtcgaagag cccctgctcg acgtaggtcg
     1081 ggatctcttg cggccgcaag aaccggactg tatcgatgcg cgggtcatcg atcgacgcgt
     1141 ggtagtcacg gtcagatcca cggctcacgc caaggtcggc cgcctcgaac aggtcgaggg
     1201 tggacgcttc gagggagcct ttgggcaaga cgacggaaag cacgtgcgtt gcctctcgga
     1261 tcggggacgg cgcagggacg gcgcggtcag ctcgacgccg gcccaccgaa gccgggaccg
     1321 acgcctgggc caacgttgcg ccgaggatgt cgcagggacc gcaggacttg cgctgcgtcg
     1381 agcgctgcca cggtcgcctc ccagcccttg tcttcagcgc tgtcgggcaa accgcagcgg
     1441 tcaagagcct gcgcaaggtt gtcacaggtg agcacaccgt tgccgatcgg ggtcgctgcg
     1501 tccagcgcaa ttcgcgtcag ccccgccgtt accgcatcgc acacgtactc gaaatgcggg
     1561 gtgccgccac ggatcacggc gccaagacac acgacggcgt cgtgctcaac ggcgagttgc
     1621 tgcgcgacga ctggtagctc caaagcgcct gcaacccgga cgacgacggg ctcgcgcacc
     1681 ccgcacgcgc gcgccgcctc gagtgcacgc tggaccagcg cgtcgaccac ctcggtgttc
     1741 cagcgggctg cgacgatgcc gaggctcagg cccgacgcat ctatcgtcgc aaggtcgggc
     1801 cggccagtcc ctgtcatgcc gcacctgccg tcatgacaga cccccgccct gatcaactga
     1861 gtcgactgag tcgactgcgt caactgaagc gtcgaccggc ggtaccgcga cgaactgcgg
     1921 gtgtccgggc aacaacaggt cgtggcccat ccggtcgcgc ttggtcagca ggtaccgcat
     1981 gttgtcgggg ttgtggtggg tcggcaaagg gacgcggccc accacactga gcccccaacc
     2041 ttcaaggccg gcccgcttgg ccggattgtt cgtaagcagg cgcatcgacc gcacgcccag
     2101 gtcgacgagc acctgcgcgc cggtgccgta gtcgcgagcg tcagcgggca ggccgagttc
     2161 gaggttcgca tcgatcgtgt cggccccgtt gtcttgcaac tcgtacgcgt gcagcttgcg
     2221 catcagccca atacctcgac cttcgtgacc gcgaaggtag aggacgactc ctcgcccttc
     2281 cagcgctatc gccgacatcg cggcgtccaa ctgcggaccg cagtcgcaac gcagcgaggc
     2341 aaagacgtcg ccagtgaggc attcagagtg agctcggacc aggacgttct caccgtcgtc
     2401 gtcatggcca ccaacctcac ccatcaccaa tgcgacgagc tcggcgtcgt cgagagtgct
     2461 gcggtagccg accgctcgcc attcgccgtg cgacgtgggc aaccgagctt cggccacgcg
     2521 tttcactgtg gtttcgtggc gccgccgata ggcgaccagg tcggcgatcg agacgatcgc
     2581 gagcgagtgc tccttcgcga actccaggag tcggggcaag cgcgccatcg agccgtcgtc
     2641 gtcgacgatt tcgctgatga cgcccgccgg ggtaaggccc gccaggacgg ccagatcgac
     2701 ggcggcctcg gtgtgaccgg tgcgccgcag cactccacca gccacagcac gcagtgggaa
     2761 cacgtgcccc ggtctcgtca actcgtaggg ctcggttgcg gagtcggcca gcacccggtt
     2821 cgtgtgcgcg cgatcggccg ccgagatgcc ggtggtgatg ccacgacgtg catcgacgga
     2881 gaccgtgtac gcggtctgct tcgcgtcttc gttgcgcgac gtcatgggtg gtagcccaag
     2941 gcggtcgagg gttgcgctct gcatcggcac acacagcacg ccgctcgtgt ggcgcaccat
     3001 gaacgcgacg agctcaggtg tcaccttcga cgccgcggcg atgagatcgc cctcgttctc
     3061 gcgatcttcg tcgtcgacga cgatcacggc acgccctgcg gcgatttcct cgattgcgcg
     3121 gtcgatcgcg tcgaacgtca tcccgacgcc tcctgtcctg cgtgttcggt cgccaccgcg
     3181 atcggtgaaa gcccacgcgg aacgagcgcg gttcgctgaa gctggcgcca cacgaagaat
     3241 ccccagatca cgaagccggc gtagatcgcg tacaacacag ccgacgggta gtagtggtag
     3301 tgcaggagct cgggtacgcc gacgatgtcg actccaatcc aacagagcca gaactcggtc
     3361 cagccgcggg ccattgcgta cgtcgcaacc atcgaaccga cgaagatcca cgagtcggcg
     3421 aggtagtaaa gccagctcgg gtggaagaac tccccctcgc cgatgctttg cagcacgggc
     3481 agcaggacgg cgaccccaag cgcggcgagt gc
//
LOCUS       DASWVF010000669         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_95314, whole genome shotgun sequence.
ACCESSION   DASWVF010000669 DASWVF010000000
VERSION     DASWVF010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95314"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1002
                     /locus_tag="VGD55_11780"
     CDS             40..1002
                     /locus_tag="VGD55_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0871071.1"
                     /translation="MPTVVIADDSPTLRRIVTSVLTREGFEVIPAEDGVEAVQAVFKS
                     HPDAVVLDVQMPRVSGYVAARLLKDDWQTSEIPIILLTSLDAASDRYWGAQTGADRYL
                     TKDFKAPDLVAAVSGAIAAAEAARGGRPAMRPDPLELDADDVLSRVCDLLDRKLFETS
                     VAAEVTGISASAKNFEETVAQVLGVLQRFVDNDVAAVLLIEERSAFVAIARDSSQLQY
                     TEFLTAAVDALGAATGTSIAVSDMDLQIMDPEEKLGADDEGRMATFLSMPLRGHGGRV
                     IGVLALSSAAKSAFSETALGTLRLVEDPAAIVIDNARLAVARAR"
     gene            1017..1538
                     /locus_tag="VGD55_11785"
     CDS             1017..1538
                     /locus_tag="VGD55_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0871072.1"
                     /translation="MSADPVDPDPADPVDVATFRRVNGKFATGISVAATEHKGIEHAM
                     TVNAFTSVSLEPLLVLICVEKITRFHAAVLATGLWALSVLSDDAEDAARWFAEKARPT
                     GGQMANYPHRPGQVTGMPVLEQAMAIIECRTRAIYDGGDHDIVLGDVVGLWSDPAPRQ
                     PLLYFGGQYRRLP"
     gene            1586..>3027
                     /locus_tag="VGD55_11790"
     CDS             1586..>3027
                     /locus_tag="VGD55_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY0871073.1"
                     /translation="MLDHEEPSHARSVPALRISLIVLGCLIVLGGAAYAAAYLYVGGG
                     VLPHTTVDGVPVGRLSESAAQAKVAAGLAPRSDAPITVTVAGQFFTLDPATAGLTVDT
                     RATIAAGVQRSASPVSLWKALFAHPKLDAAVSVDTAKLDAAVTALNAKIVGGGHDGGI
                     TFKGVTPVAIPPVPGIEIVHDKALAAIRAAYLSTSAPIALPVASVQPSVTEAAVEHAL
                     TTIAAPAVAAPINLVVGTTTLPVNPSVIAANLTFVSKGGALVPVVDSDGITAAIGSSA
                     FAGLETPAKDASFDVKSGVPVIVASVEGGTADLSALGSAITGVLTKAAPRVINVPAKG
                     IDPSFTTADAEKLGVNEMVSTFTTHHPCCAARVTNIHAIANVVNGAVVMPGATFSLNQ
                     FVGPRDTKRGFVEAPMIEDGLFENAVGGGVSQFATTLYNAVFFAGLRDITHTPHSYYI
                     SRYPAGREATVSYPAPELIFQNNEPTAIVIT"
BASE COUNT          484 a         1017 c          975 g          551 t
ORIGIN      
        1 gccacaaaga cagtgatgct cgcttcttgg aggaatcccg tgcccaccgt ggtgatcgcc
       61 gacgacagtc cgaccctgcg acgcatcgtg accagcgtgc tcacgcgcga aggattcgag
      121 gtcatccccg ctgaggatgg cgtcgaggct gtgcaggccg tgttcaagag ccatcccgac
      181 gcggtcgtcc tcgacgtcca gatgcctcgc gtttctggct acgtggcagc ccggttgctc
      241 aaagacgact ggcagacctc cgagatcccg atcatccttt tgacgtcgct cgacgcggcg
      301 agtgaccgat actggggcgc gcaaaccggc gccgaccgct acctgaccaa ggatttcaag
      361 gcacccgacc tcgtggcggc tgtgagcggt gcgattgccg cagccgaagc cgcgcgcggt
      421 gggcggcctg cgatgcgtcc cgatccgctc gagctcgacg ccgacgacgt cttgtcgcgc
      481 gtgtgcgacc tgctcgaccg caagctgttc gagacgtccg tcgccgccga ggtcacgggc
      541 atctcggcga gcgcgaagaa cttcgaggag accgtcgcgc aggtactggg agtgctccag
      601 cgtttcgtcg acaacgatgt agcggctgtg ctgctcatcg aggagcgttc tgccttcgtc
      661 gccattgcgc gcgactcctc gcagcttcag tacaccgaat tcctcacggc cgctgtcgac
      721 gccttgggcg ccgcgaccgg aacctcgatc gccgtgtccg acatggatct tcagatcatg
      781 gatccagaag aaaaacttgg ggcagacgac gaggggcgca tggcgacgtt cctgtcgatg
      841 cccttgcgtg gccatggcgg acgtgtcatc ggggtgctgg cactgtcgag cgccgcgaag
      901 agcgcgttct ctgaaacagc acttggcacg ctgcgccttg tcgaggaccc ggccgccatc
      961 gtgatcgaca acgctcgtct cgcggtcgcc cgcgctcgct gactacgctg gagcgcatgt
     1021 ccgctgaccc cgtcgacccg gacccagcgg accctgtcga cgtagcgacc ttccgccgcg
     1081 tcaacgggaa gtttgcgacc ggcatctccg tcgctgcgac ggagcacaaa ggcatcgagc
     1141 acgcgatgac cgtcaacgcg ttcacctcgg tgtcgcttga gcccttgctg gtgctcatct
     1201 gcgtcgagaa gatcacccga ttccacgctg ccgtgctggc caccggcctg tgggcgctct
     1261 cggttctctc cgacgacgcc gaagacgctg cccgctggtt cgccgagaaa gcgcgcccga
     1321 cgggcggcca gatggccaac tacccgcacc ggccagggca ggtcaccggc atgccggtgc
     1381 tcgagcaggc gatggcgatc atcgagtgcc gcacccgggc gatctacgac ggcggcgatc
     1441 atgacatcgt tctcggcgat gtggtcggtt tgtggtccga tccggcaccc cgccagccgc
     1501 tgctgtactt cggcgggcag taccgccggt taccatgacc ggactgtcct agccccgctg
     1561 ctcgtttctg gagataccgc cggccgtgct tgatcacgaa gagccatcgc acgcccgctc
     1621 ggtccccgct cttcgaatca gcctcatcgt cctcggttgc ctgattgtcc ttggcggcgc
     1681 ggcttacgcc gcggcgtatc tctacgtcgg cggcggggtg ttgccccaca ccacggtcga
     1741 tggagtgccg gtggggcggc tctcagaatc ggccgcgcag gcgaaggttg cggccggtct
     1801 cgcgccccgc tctgatgcgc ccatcaccgt gacggttgcg gggcagttct tcaccctcga
     1861 tccggccacc gccggcctga cggtcgacac ccgtgccacg atcgccgccg gcgtgcaacg
     1921 cagcgcgagc cccgtgtcgt tgtggaaggc attgttcgcg catccgaaac ttgatgccgc
     1981 tgtctcggtc gatactgcga agctcgatgc cgcggtgacc gcgttgaacg ccaagatcgt
     2041 gggcggtggt cacgatggcg gcatcacgtt caagggtgtg acgccggtcg cgatcccgcc
     2101 ggtgcccggc atcgagatcg ttcacgacaa ggcgttggcc gcgatccgcg ctgcgtacct
     2161 gtcgacgtcc gcgccgatcg ctttgcctgt cgcttcggtg cagccgagtg tcaccgaggc
     2221 ggcggtcgag cacgcgctca cgaccatcgc tgcgccagcg gtggcggcac cgatcaacct
     2281 cgtcgtcggg acgacgacgc tgccggtaaa tccttctgtg atcgcagcga atctgacctt
     2341 cgtctccaag ggcggcgcgc tggtgccggt cgtcgacagt gacggaatta ccgctgccat
     2401 cggctcttct gctttcgcag gtctcgagac gccagcgaag gacgcatcgt tcgacgtcaa
     2461 gtcgggggtg ccggtcatcg tggcgtcggt cgagggcggt accgccgatc tgtctgcgct
     2521 cgggtctgcg atcaccggcg tgctgacgaa ggccgcgccg cgtgtgatca acgttcctgc
     2581 gaagggcatc gatccttcct tcaccaccgc ggacgccgag aagctcggcg tcaacgagat
     2641 ggtgagcacc ttcaccacgc atcacccgtg ctgcgcagcg cgggtcacga acattcacgc
     2701 gatcgccaac gtcgtcaacg gtgccgtcgt gatgccgggc gcgacgttca gcctgaacca
     2761 gttcgtgggt ccgcgcgaca cgaagcgcgg cttcgtcgag gcgccgatga tcgaggacgg
     2821 acttttcgaa aacgccgtcg gcggtggtgt ttcacagttc gcgacaacgc tttataacgc
     2881 ggtgttcttc gccggtctgc gagacatcac gcacacgccg cacagctact acatcagccg
     2941 ctatccggcg gggcgcgagg ccacggtgtc gtatccggcg ccggaactca tcttccagaa
     3001 caacgagccg acggcgatcg tgatcac
//
LOCUS       DASWVF010000670         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_375469, whole genome shotgun sequence.
ACCESSION   DASWVF010000670 DASWVF010000000
VERSION     DASWVF010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375469"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VGD55_11795"
     CDS             complement(<1..1171)
                     /locus_tag="VGD55_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0871074.1"
                     /translation="MPAIFDKLMRAGEGKILRTLKAVSDQVNSVETEFETLSDAELRA
                     QTDEFKERYAGGESLDDLMPEAFATVREAARRTLGQRHFDVQIMGGAALHMGNIAEMR
                     TGEGKTLVSTLPVYLNALSGEGVHVVTTNDYLARRDSEWMGRVHRFLGLDVGVILSQM
                     PSAERRAAYAADITYGTNNEFGFDYLRDNMALSAEELVQRGHNFAVVDEVDSILIDEA
                     RTPLIISGQGHDATELYGQFAKIIPRLVKDEDFTVDQKAHAAPITEKGVAKVEKMLGV
                     TNLYDQRNLELTHQLNAALKAWNLFHKDQQYIVKDGEVVIVDEFTGRLMYGRRYSDGI
                     HQAIEAKEGLDVRSEDQTLATITFQNYFRLYDKLAGMTGTAKTEEREFREIYGLNV"
     gene            1314..2510
                     /locus_tag="VGD55_11800"
     CDS             1314..2510
                     /locus_tag="VGD55_11800"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0871075.1"
                     /translation="MPRPTAREFMAVELSADEARRFALAAQHLVGSPDRRAGVAGLLG
                     SLRAVQLDTISVLARSHELVPYARLGAVGRPKIDAAYWGGDAFEYWAHAACVMPMSQW
                     PLFAFRRRRFLGRSKEWGQHSGAPSKAVLARVRAEGPMTATDLGGAKKSAQWWGWSEV
                     KRSAEFLLATGQLVCTRRVGWRRIYDLPERAIPADVLHDDLDDDQCQVALIQLAAGCL
                     GVATGSDLADYFRLKKLDVARLLPQTGLVPVAVRGWAAPAWAQADTLDRGLPRGRHRT
                     TLLSPFDSLVWDRARTARIFGLDHRLEAYVPKPLRQHGYFSMPVLAGGQLIARVDPAR
                     VAGTLTARQVTVIPEHATAARLPATSVAIRDALSEAAAWVGCSAIAIERVTPESAVDR
                     VRLAVA"
     gene            2518..>2863
                     /locus_tag="VGD55_11805"
     CDS             2518..>2863
                     /locus_tag="VGD55_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY0871076.1"
                     /translation="MATAEAVAGVTCLAATGIGVVAIAVLHVLPTGLSPAHSAVSQYG
                     ITRYRLGYRVQTIAFAVAGGAAAAGLAEAVPGRAPALIALLTVFVVARAIISWYPMDE
                     PGGEPTNHGRMHG"
BASE COUNT          393 a          932 c         1000 g          538 t
ORIGIN      
        1 ctacgttgag cccgtagatc tcgcggaact cacgctcttc ggtcttcgcg gtacccgtca
       61 tcccggccag cttgtcgtag aggcggaagt agttctggaa cgtgatcgtc gcgagcgttt
      121 ggtcctcgct gcggacgtcg agcccttcct tcgcttcgat cgcttggtgg atgccgtcgg
      181 agtaccggcg cccgtacatc agccggccgg tgaactcgtc gacgatgacg acctcgccgt
      241 ccttgacgat gtactgctgg tccttgtgga acaggttcca cgccttgagc gcggcgttga
      301 gctggtgggt gagctcgagg ttgcgctgat cgtagaggtt cgtgacgccg agcatcttct
      361 cgaccttcgc gacgcccttc tcggtgatcg gcgccgcgtg cgccttctgg tcgaccgtga
      421 aatcttcgtc cttgaccagg cgcgggatga tcttggcgaa ctgcccgtag agctcggtcg
      481 cgtcgtggcc ctggccgctg atgatcagcg gcgtacgcgc ctcgtcgatc aggatcgagt
      541 cgacctcgtc gacaacggcg aagttgtgcc cgcgctgcac gagttcttcg gcgctcaggg
      601 ccatgttgtc gcgcaagtag tcgaagccga actcgttgtt ggtgccgtag gtgatgtcgg
      661 ccgcgtaggc ggcgcgccgt tcggccgacg gcatctgcga caggatcacg ccgacatcga
      721 ggccgaggaa ccggtgcacc cggcccatcc actcagagtc acggcgggcc aggtagtcgt
      781 tggtcgtgac gacgtggacg ccctcgccgg acagcgcgtt caggtacacc ggcagcgtcg
      841 agacgagcgt cttgccctcg ccggtgcgca tctccgcgat gttgcccatg tgaagggcgg
      901 cgccacccat gatctggacg tcgaagtgcc gctggccgag cgtcctgcga gccgcctcgc
      961 gaaccgtcgc gaacgcctct ggcatcaggt cgtcgaggct ctcgccgccc gcgtaacgct
     1021 ccttgaactc atcggtctgc gcccgcaact cggcgtccga cagcgtttca aactcggttt
     1081 cgaccgagtt gacctggtcg gacactgcct tgagcgtgcg aaggatcttc ccttcgccgg
     1141 cgcgcatgag cttgtcgaag attgcgggca cgaagctttg ctcctcgaaa cggtcctgga
     1201 cgatacggga cggcgggggc gtgcggtccg tccttccgcc atcgtaggcg aggctgaggc
     1261 gtcccggcgc gtagcgccca cgtggcgtcg gtggtgccac ctacgatccg accatgccca
     1321 gacccaccgc gcgggaattc atggccgtgg agctgtcagc cgacgaggcc cggaggttcg
     1381 cgctggctgc tcagcatttg gtcggctccc ctgaccgccg ggccggcgtc gcggggctct
     1441 tggggtcgct tcgggcggtc caactcgaca cgatctcggt gttggcgcgg tcgcacgagc
     1501 tcgttcccta tgcacggctc ggcgcggttg gccgcccaaa gatagacgcc gcgtactggg
     1561 gtggcgacgc gtttgaatac tgggcgcacg cggcgtgcgt catgccgatg tcgcagtggc
     1621 cgttatttgc gtttcgccgg cgtcgcttcc tgggccggtc caaggaatgg ggtcagcact
     1681 ccggcgcgcc gtcgaaagcg gtgctagctc gggtgcgggc cgagggtccg atgaccgcga
     1741 cagacctcgg cggggcgaag aagtcggcgc agtggtgggg ctggtcggag gtgaagcggt
     1801 cggccgaatt cttgctggcg actgggcagc tcgtgtgcac ccgtcgggtc ggctggcggc
     1861 ggatttacga cctgcccgaa cgggcgattc cggccgacgt gctgcatgac gatctcgacg
     1921 acgaccagtg ccaggtggcg ctgatccagc tggccgcggg ctgtctcggt gtcgccaccg
     1981 gctccgacct cgccgactac ttccggttga agaagctcga cgttgcgcga ttgcttccgc
     2041 agaccgggct ggtgcctgtc gccgtgcgtg ggtgggcggc tcccgcgtgg gcacaggcgg
     2101 acaccctcga ccggggtttg ccgcgcggcc gtcaccgcac cacgctgctc tccccgttcg
     2161 attcacttgt ctgggaccgc gcgcggacgg ctcggatctt cgggctcgac caccggctcg
     2221 aggcctacgt gccgaagccg ttgcgccagc acggctactt ctcgatgcca gtactcgccg
     2281 gtgggcaact gatcgcgcgc gtcgacccgg cgcgggtcgc tggcacgttg accgcccggc
     2341 aggtgaccgt cattcctgag cacgcgacag cggccaggtt gcccgcgacc tcggtggcta
     2401 tccgcgacgc cctctcagag gccgctgcgt gggtgggctg cagcgcgatt gcgatcgagc
     2461 gcgtcacccc tgagagtgct gtggatcggg tacgactcgc ggtggcatga tccccgtgtg
     2521 gcaactgcag aggccgttgc aggcgtgacc tgtttggccg ccaccggcat cggtgtcgtg
     2581 gcgatcgcgg tgctccacgt actgcccacg gggttgtccc ccgcgcacag cgcggtgagc
     2641 cagtacggga tcacccgcta ccgcctcggt taccgagtgc agaccatcgc gttcgcggtc
     2701 gcgggtgggg ccgcagcggc cggcctcgca gaagcggtgc ccgggcgggc gccggcgttg
     2761 atcgccctgc tcaccgtgtt tgtcgtggcg agggcgatta ttagctggta cccgatggac
     2821 gagcccggtg gcgaacccac caatcacggg cgcatgcacg gcg
//
LOCUS       DASWVF010000671         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_146291, whole genome shotgun sequence.
ACCESSION   DASWVF010000671 DASWVF010000000
VERSION     DASWVF010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146291"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..516
                     /locus_tag="VGD55_11810"
     CDS             58..516
                     /locus_tag="VGD55_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0871077.1"
                     /translation="MAELSTVDGLAQLSFLVQSTLDRRAAEHELSMIQTRLLGVLRDR
                     TPTMNELAKLLDLDKSSVTGLVDRAERRGLVERTTSAADGRVVLVSLTRAGRSLVSAV
                     AKRFEADVSALLKHLPVADRAALSGLITRVLVAHAAREGVDLFPMYPRPS"
     gene            763..1698
                     /locus_tag="VGD55_11815"
     CDS             763..1698
                     /locus_tag="VGD55_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871078.1"
                     /translation="MARYNHPPNWPKPPKGWTPPQGWKPNPKWPPAPSGWQFWIDEPK
                     PASSAAAAPVTPAAAALAPAAAVAPAYRGSRRKSPFGAITGAVSAALLARGHRVRSTL
                     VFSAVCIVLLAVIAVIVVGVPKKSTNVGKVTSGALPTGQARPASAGTGSAKPSASAPT
                     SQPPVSPTAAVTPSATTATTPLSAAPLVATSASAIPTPTPTPTPTSTPTPTPNKPAPP
                     VPTTCVASVSDATPHHDEVVNVIVATAPGAHVSVTAHYHTERTTHHAVADATGQASVP
                     FDVLDAIYGFNVRVTVVASGAGGTLDCATSYTPRP"
     gene            1810..2073
                     /locus_tag="VGD55_11820"
     CDS             1810..2073
                     /locus_tag="VGD55_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871079.1"
                     /translation="MPTVIGHHDVKDTAHWLASPKREEFFGPLGVTNIRTFVNPQNRN
                     QVAVLMDVADLDVLMAAMQEPAAADAMASDGLLPETLVILIEE"
     gene            2256..2717
                     /locus_tag="VGD55_11825"
     CDS             2256..2717
                     /locus_tag="VGD55_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871080.1"
                     /translation="MTNTELLSDLKVAFTPSSLDVRTEIELDAGRRRVFDALLHIAAW
                     WPDRGRHGSRIVFEPHVGGRFFESCDDGCGILLGQVSRLLTPDEFAIEGSFGLDQPVC
                     ALWSVRLDSGGTKTVLHGQFRAFGALDDDLRMATVTAWERRYAALAQYVMA"
     gene            2740..3900
                     /locus_tag="VGD55_11830"
     CDS             2740..3900
                     /locus_tag="VGD55_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY0871081.1"
                     /translation="MPNPSLADVVAVIEGLYPPSLAAEWDAIGLVCGDPSQPVGRILF
                     AVDPVDPVVDEAIDRGADLLVTHHPLFLRGTSSVAATTVKGRSVHRLIRAGIGLHVAH
                     TNADHARPGVSDALAAALGLIDTRPLEPLPAPALDKVVVFVPEADAEKLIDALSAARA
                     GELGDYSRCAWTTAGVGTFVPGLRANPAVGTVGEVASVAETRVEMVFARGARHDVVSA
                     LRAAHPYEEPAFDVVELASWPGDVGTGRVGEVPATTLDAFASVVGSALPSTGGGGRVA
                     GDLERVVRRVAVCGGAGDAYLRHASASGSDVFVTSDLRHHVVSEHLADGGCAVIDMPH
                     WATEWPWLGDAADRVRAGLEARGTTVVTEVSTTPTDPWVARVDAASPRKDSH"
     gene            3936..>4551
                     /locus_tag="VGD55_11835"
     CDS             3936..>4551
                     /locus_tag="VGD55_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871082.1"
                     /translation="MQALDSSLDRLDHRRATLPELATIEALSSRVREVSDALVVAETL
                     ESDLGREQRKAEGDVDQVRARSDRDQQRLDAGQVSSPRELENLQSEIGSLARRRSDLE
                     DVVLEIMERREAAQADMSSLGAEREKLSAELVAVTSRRDELFGEIDAEIAASRSARAA
                     VAPDVGVELLTLYEKIRASSDGVGAAALHRGRCEGCHLTVNPSEL"
BASE COUNT          717 a         1554 c         1507 g          773 t
ORIGIN      
        1 ttcgtgtcac gtacaaacat agttggtgcc acgtactaaa tcaactacga tgtagtcatg
       61 gcagaactga gtacggtcga cgggctcgct cagctttcgt tcctggtcca gagcacgctc
      121 gaccgccgag cggccgagca cgagctgtcg atgattcaga cgcgacttct cggcgtcctg
      181 cgcgatcgca ccccgaccat gaatgaactg gccaagctgc tcgatctcga caagtccagc
      241 gtgacgggtc ttgtcgaccg cgccgagcgg cgcggcttgg tcgagcgcac tacctcagcc
      301 gcagatggcc gggtggtgct ggtttctctg acccgcgccg gtcgctcgct ggtgtcggcg
      361 gtcgcaaagc gattcgaggc cgacgtgtca gcgctgctca aacacctgcc agtcgccgat
      421 cgggctgccc tgtcgggatt gattacccgt gtcctggttg cccacgccgc tcgtgaaggc
      481 gtcgatctgt tcccgatgta tccgaggccg tcctagcgac tcttatgagg ggcgccttcc
      541 cttaagggcg acgcctgacc cgacgaaaga accaccgcag tgccagcagg ccaatgaacc
      601 gcagcagcaa gggcataccg gccagcgtaa gggtcggaga ggccaccggg catcgcgagc
      661 gcattcggta ttcgtccgga cggcggcgcg acacgccctg cgattcgtgg accggccgga
      721 tcgactccca tacgctccga gcgttgatct tgaagggaat tgatggcgcg ctacaaccat
      781 ccgccgaact ggccgaagcc ccccaagggc tggacgccac cccagggatg gaaaccgaac
      841 ccgaagtggc cacctgcgcc gtcgggttgg cagttttgga ttgacgagcc caagccggcg
      901 tcgtcggctg ccgctgcgcc cgttactccc gcggccgcgg ccctcgcgcc cgccgcagcc
      961 gtcgcgcccg cttaccgcgg ctcccgccga aagtcgccgt tcggcgctat caccggggcc
     1021 gtctccgcag cgctcctagc gcgagggcac cgagtgcgca gcacgctcgt ctttagcgcg
     1081 gtgtgcatcg tcctgctcgc tgtgatcgcg gtcatcgtgg tcggcgtccc gaagaaatcg
     1141 acgaacgtcg gcaaggtcac ctccggcgcg ttgccgacgg ggcaggcccg gccggccagc
     1201 gcaggtaccg gatcggcgaa gccgagcgcc agcgcgccga cgtcgcaacc tccggtgagt
     1261 cccacggcag cagtgacgcc atcagccacg accgcaacga cgccccttag cgccgcgccg
     1321 ctcgtcgcta catccgccag cgcaatcccg acgccaaccc cgacgcccac gccgacttcg
     1381 acgcccacgc cgacgccgaa caagccggcg ccgccagtac cgacgacgtg tgtcgcgagc
     1441 gtctctgacg cgactcccca ccacgacgag gtggtcaacg tcatcgtggc gacggcgccc
     1501 ggcgcccatg tcagcgtgac cgcgcactac cacacggagc gcacgaccca ccacgccgtc
     1561 gccgacgcca ccggccaagc gtccgtgcca ttcgatgtct tggacgcgat ctacggattt
     1621 aacgtgcggg tgaccgtcgt ggcgtccggc gccggaggca cgctcgactg cgcgaccagc
     1681 tacacgccgc gtccataagc ggctgcggcc cgctcggctc tagcgcgcac ccaggcgcgg
     1741 acctacgatc cgcagaccag gccattgatt gctgcctggc ccaccgcaag aaacgacaag
     1801 ggagtcgcta tgcccacggt gatcggccac cacgatgtca aagacacggc tcactggctc
     1861 gcgtcaccga agcgcgagga gttcttcgga cccctcggtg tcaccaacat ccgcacgttc
     1921 gtgaatccgc agaaccgcaa ccaggtcgcg gttctgatgg atgttgccga cctcgatgtc
     1981 ttgatggccg cgatgcagga gccagcagcg gccgatgcga tggcctccga cgggctgctt
     2041 ccggagaccc tggtgattct gatcgaggag taggacggcc gccgcgcggc gtcctgcaac
     2101 ttcttgccct acccgcacgt catcgaaata cggcgtcaca accgctcacc ccgggcggac
     2161 gccgagtggc ccggttcgct tcggcagccg ggccacgacc aactcaggga cgacgaccct
     2221 ggatttcggc accgggtcac ggcacgatga gggccgtgac gaacaccgag ttgttgagtg
     2281 atctcaaggt cgcgttcacg cccagttcac tcgacgtccg aacagagatc gagttggacg
     2341 ccggtcggcg gcgagtcttc gatgcgttgc tgcacatcgc ggcttggtgg ccagatcgcg
     2401 gcaggcacgg ctcgcggatt gtgttcgaac cacacgtcgg cggccggttc ttcgagagct
     2461 gtgacgacgg ctgcggaatc ctgctcggtc aggtctcgcg actgctgaca cccgacgagt
     2521 tcgcgatcga gggatcgttt ggtctcgacc agccggtgtg cgcgctgtgg tcggttcgcc
     2581 tcgactccgg tggaacgaag acggtgctgc acgggcagtt ccgtgccttc ggggccctgg
     2641 acgacgacct gcggatggcg acggtgaccg cgtgggagcg gcgttacgcg gcgctcgcgc
     2701 agtacgtgat ggcctgagcc tgccgttaac ctgtgagttg tgccgaatcc gagtctcgct
     2761 gacgttgtcg cggtcatcga ggggctgtac ccgccgtcac tggcggccga gtgggacgcc
     2821 atcgggctgg tctgcggcga cccgtctcag cctgtggggc gcatcttgtt cgccgtcgat
     2881 cccgtcgatc ccgtcgtcga cgaggccatc gatcgcggtg ccgacctgct cgtgacacat
     2941 cacccgctct tcctgcgcgg gacgtcatcg gtagcggcga cgaccgtgaa aggccgctcg
     3001 gttcaccggt tgatccgcgc cggtatcggc ctgcacgtcg cgcacaccaa cgctgaccac
     3061 gcgcggcctg gagtatccga tgcgctcgcg gccgcgctcg gactgatcga cacccgcccg
     3121 ctcgagccgc tgcccgcgcc cgcgctcgac aaggtcgtcg tcttcgtccc tgaggcggac
     3181 gccgagaagc tgatcgacgc gctctcggcc gctcgagcgg gcgagcttgg tgactactcg
     3241 cgctgcgcgt ggaccaccgc tggtgtcggg acgttcgttc ccggcttgcg ggcaaatccc
     3301 gcagtcggaa ccgttggcga ggtcgcaagc gttgccgaga ctcgcgtcga gatggtgttc
     3361 gcgcgtggcg cgcgccacga cgttgtcagc gcgttgcggg cggcgcatcc gtacgaggag
     3421 ccagctttcg acgtcgtcga actcgcctcg tggccaggcg atgtcggcac cggtcgggtt
     3481 ggtgaggttc cggctacgac gctcgacgcg ttcgcttcgg tcgtcggttc cgcgttgccg
     3541 tcgaccgggg gcggcggccg cgtcgcgggt gatcttgagc gggtcgtgcg gcgcgtcgct
     3601 gtgtgtgggg gtgccggcga tgcgtacctg cgacacgcct cggcgtccgg ctcagatgtc
     3661 tttgtcactt ctgatttgcg gcatcacgtg gtctccgagc atctcgccga cggcggttgc
     3721 gcggtgatcg acatgcctca ttgggcgacc gagtggccct ggctgggtga cgctgccgac
     3781 cgagtccgcg ccggactcga agcgcgcggc actacggtgg tcacggaggt ttcgacgacg
     3841 ccgaccgatc cctgggtcgc ccgagtcgat gccgcctcac cccgaaagga ttcgcactga
     3901 acgccgatcc cgccagccag gcccgcctgc tcgacttgca ggcgctcgac tcgagcctcg
     3961 atcggctcga tcacaggcgg gcgactctgc ccgagctcgc gacgatcgag gcgttgagtt
     4021 cgcgggtccg tgaggtcagt gacgcactcg tcgtcgccga gacgctcgag tctgacctcg
     4081 gtcgcgaaca gcgcaaagcc gagggggacg tcgaccaggt gcgggcccgt tcggaccgtg
     4141 accagcagcg actcgacgcc ggtcaggtca gctccccgcg cgagctcgag aacctgcaat
     4201 ccgagatcgg atcccttgcc cgccggcgtt cggatctcga ggacgtcgtg ctggagatca
     4261 tggagcgccg cgaagcagcg caggccgaca tgtcgtcgct cggcgccgaa cgcgagaagc
     4321 tctccgcgga gctcgttgct gtcacctcac gccgcgacga gttgttcggc gagatcgacg
     4381 ccgagatcgc tgcgagccgc tccgcccgcg ccgccgtcgc tcccgatgtc ggagtcgaat
     4441 tgcttaccct gtatgaaaaa atcagagctt cgtcggatgg cgtcggcgcg gcagcgttgc
     4501 atcgcggccg gtgtgagggt tgccatctga cggtgaaccc gtctgagctc g
//
LOCUS       DASWVF010000672         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_197426, whole genome shotgun sequence.
ACCESSION   DASWVF010000672 DASWVF010000000
VERSION     DASWVF010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197426"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGD55_11840"
     CDS             <1..803
                     /locus_tag="VGD55_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0871083.1"
                     /translation="SLLAILGVPAPRDVVQHTGEIMRKVAFDDKTPTAIAGAVAAARE
                     NARGAREAISSEMWECLNVTWRTMPERRRAAARLGPNTYLDYVRERTAMFSGLAESTL
                     SHDDGWHFLVLGRSLERADMTARLLSVQLLTADQPVTWVTVLSACGGYEAFLHTYGGM
                     VDRARAVQFLALDRSFPRSVLHSLSTAEQCLAALNPQLARGLVADPARRIVGQARTAL
                     EYVEVADLVHDLSEYLVPVQEACASASQAVSRRYFTYATPLAWAQELV"
     gene            800..1645
                     /locus_tag="VGD55_11845"
     CDS             800..1645
                     /locus_tag="VGD55_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0871084.1"
                     /translation="MSWRLKVVHTTRFAYATAAHASYNEARLTPPTLPSQTTLESRIE
                     IEPTAPVYRYHDYWGTQVSAFDLDNEHSELTVRATSTVETVKPIARIEQPLGWLELQH
                     PKATDKFAEYLAQTTRSAVDDELVAEVREQIQGMSPTETALRVSSWIHDAVEYVPGST
                     GVQTSAREAWHRRQGVCQDIAHLSAGLLRRFGIPARYVSGYVHPKRNATIGETVKGQS
                     HAWVEWWDGDWTGHDVTNDQPIGVHHVVVARGRDYGDVAPLKGVYQGRSASHLAVTVD
                     VTRLS"
     gene            1646..2482
                     /locus_tag="VGD55_11850"
     CDS             1646..2482
                     /locus_tag="VGD55_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0871085.1"
                     /translation="MSAELSIGGTHVGCDLVLDVVEPSTVVLSIAPAASAGVLDSEQF
                     SLTCNGDPRDVYPRRIPSAHGASLHGLELPVGRVSVHYQAHLTTGGPATSMLSDEAEL
                     LKYRRPSRFCPSDRLTGFASAELGHLSAGRGQLDGLVVWVARRLAYVSGSSGPLDTAV
                     ETLLAGQGVCRDYAHLVVAMARALDLPARCASVYAPGLWPMDFHAVAEVYVDGKWNVV
                     DATYLAPRESLVRIATGRDAADTAFMTTLTGSVEMLEVDVRASVDGELPRDSFTDAIV
                     LR"
     gene            complement(2453..2929)
                     /locus_tag="VGD55_11855"
     CDS             complement(2453..2929)
                     /locus_tag="VGD55_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871086.1"
                     /translation="MFITNHVLSGALIGLAAPRRPASVALVAAGSHFALDSVPHWGSR
                     DDVSFRKVAIVDGLVGLATMRAVMGVVPRGTRVAVLAGMLGAAFPDSDKPALMFFGRS
                     PFPAPVDVFHAKIQRESSHGIRTEIKAALAMVAAVRVLSWLRGVTAELAEDDRVGE"
     gene            complement(2962..>3421)
                     /locus_tag="VGD55_11860"
     CDS             complement(2962..>3421)
                     /locus_tag="VGD55_11860"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0871087.1"
                     /translation="ETTALALSNVYGPRQDPHGEAGVVAIFGNALLQGRSAVIYGDGS
                     SIRDYVFVEDVADAFARAAGDKGNGRRFNIGTGVGTSVRALHTAVADAALAPDDPVFA
                     WPRLGELQAITLDVSAASAALGWVPRTALPEGLVKTIHWIQTQPDRPPAK"
BASE COUNT          590 a         1150 c         1130 g          551 t
ORIGIN      
        1 ggtcgttgct ggcaattctc ggcgtccctg cccctcgcga tgtcgttcaa cataccggcg
       61 agatcatgcg caaggttgcc ttcgacgaca agacgccgac cgccatcgcc ggcgcggtcg
      121 cggccgcgcg cgagaacgca cgcggcgctc gtgaggccat ctccagtgag atgtgggagt
      181 gcctgaacgt cacctggcgc accatgccag aacggcgaag ggccgccgca aggctcggcc
      241 caaacaccta tctcgactac gtccgcgaac gcaccgcgat gttctccgga ctcgccgagt
      301 cgacgctgtc gcacgacgac ggctggcact tcctggtgct tggccgcagt ctcgaacgcg
      361 ccgacatgac ggcgcggctg ctgtcggtgc aactgctcac cgccgaccaa ccggtcacct
      421 gggtaaccgt gctctcggca tgcggcggct acgaagcgtt tctgcatacc tacggtggca
      481 tggttgaccg cgctcgcgcc gtgcagttcc ttgcactcga ccggtcgttc ccgcgttcgg
      541 tgctgcactc gctgtcgact gctgagcagt gcctggccgc cctgaacccc cagctggccc
      601 gtgggctggt cgccgatccc gcgcgccgca tcgtcgggca ggcccgcacg gcgttggagt
      661 acgtcgaggt cgccgacctc gtacacgatc tgagcgagta cctcgtaccc gtacaagaag
      721 catgcgccag cgcgagccag gcggtatccc gcaggtactt cacgtatgca accccccttg
      781 cctgggcaca ggagctggta tgagctggcg attgaaggtc gtgcatacga cccggttcgc
      841 gtacgcgacc gccgcccacg cgtcgtacaa cgaggcccgg ctcactccgc cgacgctgcc
      901 gtcgcagacg acgcttgagt cgcggatcga gatcgagccg accgcgccgg tctaccgcta
      961 ccacgactat tggggtactc aggtttccgc attcgatctc gacaacgagc actccgagct
     1021 cacggtgcgg gcgacgagca cggtcgagac ggtgaagccc atagcgcgca tcgagcaacc
     1081 gcttggctgg ctcgagctgc aacacccgaa ggcgaccgac aagttcgccg agtacctcgc
     1141 gcagacgaca cgctcagcag tcgacgacga gctcgttgcc gaggtgcgcg agcagatcca
     1201 aggcatgtca ccaacggaga cggcactgcg ggtctcgtcg tggattcacg acgcggttga
     1261 gtacgtgccg ggctcgaccg gcgtgcagac tagcgcgcgc gaggcgtggc accgaaggca
     1321 gggtgtctgc caagacatcg cgcacctcag cgctgggctt ctccggcgat tcggaatacc
     1381 tgcccgctac gtgtctggtt acgtgcaccc gaagcgcaac gcgacgatcg gtgagacggt
     1441 caagggtcag agccacgctt gggtcgaatg gtgggacggc gactggaccg gtcacgatgt
     1501 caccaatgac cagcccatcg gcgtccacca tgtcgttgtt gcacgaggtc gtgactacgg
     1561 cgacgtcgca ccactgaagg gtgtgtatca gggtcggtcg gcgagccacc tcgcggtcac
     1621 ggttgacgtc accaggctga gttgagtgtc ggctgagttg agtatcggcg gcacgcacgt
     1681 cggctgcgac ctcgtgctcg atgtcgtcga gccgtcgacg gtcgtgctga gcatcgcacc
     1741 cgcggcgagt gctggcgtgc tcgacagcga gcagttcagc ctgacgtgca acggcgatcc
     1801 gcgcgacgtc tacccgcgcc ggattccgag cgctcacggt gcttcgttgc acgggcttga
     1861 gcttccggtc ggacgcgtca gcgtgcacta ccaggcgcat ctcacgacgg gtgggcctgc
     1921 gacatccatg ctcagtgacg aggcggagct gctgaagtac cggcgtccga gcaggttctg
     1981 cccatcggat cggctcaccg gcttcgcgag tgccgagcta ggccacctct cggctggccg
     2041 tgggcagctc gacggcctcg tagtttgggt cgcgcgaagg cttgcgtacg tgtcgggatc
     2101 gagtggtcca ctcgacaccg cggtcgagac attgcttgcg gggcagggtg tctgccgcga
     2161 ctacgcacac ctcgtcgtcg cgatggcgcg cgcgctcgac ctgccggcgc gatgtgcgtc
     2221 ggtgtacgcg ccgggcttgt ggccgatgga tttccacgcg gtcgcggagg tgtacgtcga
     2281 cggcaaatgg aacgtggtcg acgccaccta cctcgcgccg cgcgagagcc tcgtacgcat
     2341 cgcgactggc cgcgacgcgg ccgacacagc cttcatgacc acactgacgg gcagcgtcga
     2401 gatgctcgag gtcgacgtcc gggccagcgt cgacggtgag ttgccacgcg actcattcac
     2461 cgacgcgatc gtcctccgct aactcagccg tcaccccgcg cagccagctc aggactcgca
     2521 cggctgccac catcgcgagc gcggctttga tctccgtgcg gatcccgtgg ctcgactcgc
     2581 gttgaatctt ggcgtgaaac acatcgaccg gcgcagggaa tggcgagcga ccgaagaaca
     2641 tcaacgcggg cttgtcggaa tcgggaaagg ccgcaccgag catgccggcc agcaccgcga
     2701 cccgcgtgcc gcgcggcacc acacccatca cggcccgcat cgtcgccaag ccgaccaacc
     2761 cgtcgacgat cgcgaccttg cgaaacgaca cgtcgtcgcg gctgccccag tgcggcacgg
     2821 agtcgagtgc gaaatggcta cctgcagcga caagagcaac tgaggccgga cgccgaggag
     2881 cggccaatcc gatcaaggcg cccgacagca cgtggttggt gatgaacatg cggccagcct
     2941 aggcgagcag cctgcccgcg ctcacttggc gggcgggcgg tctggttgcg tctggatcca
     3001 gtggatcgtc ttgacgagac cttcaggcaa ggcggtgcgc ggcacccaac cgagggcggc
     3061 actcgcagcc gacacgtcga gcgtgatggc ctgcaactca ccgaggcgcg gccacgcgaa
     3121 caccggatcg tcgggggcga gagcagcgtc ggcgacagcg gtgtgcaggg cacgcactga
     3181 cgtgccgact ccggtgccaa tgttgaaccg acgcccattg cccttgtcgc cggccgcccg
     3241 tgcgaacgca tcggcgacgt cctcgacgaa cacgtagtcg cggatcgacg agccgtcgcc
     3301 gtagatgacc gccgaccgac cttgtaacaa cgcgttgccg aaaatcgcga cgacccctgc
     3361 ctcgccgtgc ggatcctggc gagggccgta cacgttcgac agcgcgagtg ccgtcgtctc
     3421 g
//
LOCUS       DASWVF010000673         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_452490, whole genome shotgun sequence.
ACCESSION   DASWVF010000673 DASWVF010000000
VERSION     DASWVF010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452490"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGD55_11865"
     CDS             complement(<1..311)
                     /locus_tag="VGD55_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0871088.1"
                     /translation="MIVVDASVLANAVGDDDDAGRQARDLLRDSGELAAPDLVDVETT
                     AVLRRRWLARALTDRRFEEAIDDLKNMPIQRYPTLALIRRAFELRENVTAYDACYVAL
                     A"
     gene            complement(308..538)
                     /locus_tag="VGD55_11870"
     CDS             complement(308..538)
                     /locus_tag="VGD55_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871089.1"
                     /translation="MTNVLVRDLTAETHRELVRRADARGQSLQQYLAAELTRLASSPT
                     LDDVLARIETHSGGRVGLAQAVADLADERGRR"
     gene            704..>2174
                     /locus_tag="VGD55_11875"
     CDS             704..>2174
                     /locus_tag="VGD55_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871090.1"
                     /translation="MRHKRAAVAVTALLLSGGVPAGLASAAPIRGGNAVISPRSASNG
                     TDVIDLGNLGGGASDAGAINDQGEVVGQSVGHAFSWTAAGGMVDLGDPFGTLPASTYA
                     AGVNDAGVVVGDSTTLPPFTEAFAFTWTPAGGIALVGGLHFGDTPDAVAINSQGQMAG
                     NNTTPTGTKHAVFWTAGHAPVDIGVLGGSSGGSSATALNDNGQVVGTSSTTTADDAFS
                     WTQAGGIVDLGSLGGGSSDAVALNNAGEVVGFSYNSHAQSHAFSWTAGGGMVDLGTLG
                     GTMSQPFGINSYGQVVGWSTNASGVADAFSWTQSGGMVDLGNFGGAGGSLAMAVNNGG
                     EVIGRSQTASGDWDPFSWTSAGGMVDLGNLPGDWGYPVAINDAGQIVGYSFQAHNLGP
                     DEATLWQPQNFGGGGGGGGGGGSGGGPAVGVVSAGGSLSSDPVGTTPTTSNPLVITVT
                     SPLAGTVTIDKTPPNTPVVGYRQLGLGATISAPVTSTAAP"
BASE COUNT          402 a          680 c          714 g          378 t
ORIGIN      
        1 tcggcaagag cgacataaca agcgtcgtac gccgttacgt tttcgcgtaa ctcgaacgct
       61 cgacggatga gcgccagtgt ggggtatcgc tgaatcggca tgttcttgag atcgtcaatc
      121 gcttcttcga atcgtcggtc cgtcaaggcg cgggcgagcc agcgacgacg aagcaccgcc
      181 gtggtctcga catcgacgag atccggggcg gcaagctctc cggaatcacg gagtaggtcg
      241 cgagcttgtc ggcccgcatc atcgtcgtcg cctaccgcgt tcgccagcac tgaagcatcg
      301 accacgatca ccgacggcca cgttcatccg ccaggtccgc gactgcttgg gcgagaccga
      361 cgcggccacc gctatgggtc tcgatacgcg ctaagacgtc gtccaaggtc ggggaactcg
      421 ccaaccgagt cagctcagca gcgaggtatt gctgcaggga ctgaccgcga gcatcagcgc
      481 ggcgaactaa ttcgcgatga gtctcggcgg tcaggtcgcg cacgagcaca ttggtcatgc
      541 ttgcattatg ctatcaactt ctcttacatg caagcggccc ggtacacacc gttcgcacca
      601 tttgttccgc ggacgtcacc cgcccggcat cacgaaccgt ctgcggtcat ctcgtgcggg
      661 tgcaagactg cgggccgcag ccgtgcctcg aggagaaatt gaaatgcgcc acaaacgtgc
      721 cgccgtcgcc gtcaccgccc ttcttctcag cggaggcgtc ccggccgggc tagcgtctgc
      781 agcgccaatt cgcggcggca atgccgtgat atcaccgcga tcagcaagca acggcaccga
      841 cgtcatcgac ctcggaaatc tcggcggcgg cgcaagcgac gctggggcta tcaacgatca
      901 gggcgaggtg gtgggccaaa gcgtggggca cgcattctcg tggactgcgg ccggcggcat
      961 ggtagatctc ggcgacccct tcggaacgct gcctgcgtca acctacgccg ccggcgtgaa
     1021 cgacgccgga gtcgtggtcg gtgacagcac taccctgccg ccgtttactg aggcattcgc
     1081 attcacttgg actccagccg gaggcattgc cctagtcggc ggtcttcatt tcggcgatac
     1141 gcccgatgcc gtcgcgatta acagccaggg tcagatggcc ggcaacaaca caacgcctac
     1201 gggaacaaaa catgctgtct tttggacggc gggacatgcg cccgtcgaca tcggtgttct
     1261 gggcggctca tccggaggca gtagcgctac agcgctgaac gacaacggcc aggttgtcgg
     1321 cacgagttcg acgacgacgg cggacgacgc gttctcctgg acgcaagctg gcgggatcgt
     1381 cgatctcggg agcctaggtg gtggcagcag cgacgcggtc gcgctcaaca acgcgggcga
     1441 ggtcgtcgga ttcagctaca acagccatgc gcagtcgcac gcgttctcgt ggactgccgg
     1501 tggcggcatg gtcgacctcg gcaccctcgg cggcacaatg agccagccgt tcggcatcaa
     1561 cagctatggc caggtagtcg ggtggagcac caacgcgagc ggggtcgcag acgcgttctc
     1621 atggacgcag agcggcggga tggtcgacct cggcaacttc ggtggcgccg gcggaagtct
     1681 tgcgatggcc gtgaacaatg gtggcgaggt catcggtcgt agtcagaccg ccagcggtga
     1741 ttgggacccg ttctcgtgga cgtcggctgg cgggatggtc gacctcggca acctgcccgg
     1801 cgactgggga tatccggtcg ctatcaatga cgccggccag atcgtcggct atagctttca
     1861 ggcacataat ctcggcccag acgaagcgac gctttggcag ccgcaaaact ttggtggtgg
     1921 cggcggtggt ggtggcggcg gaggcagcgg tggcggtccg gccgtcgggg tcgtgagtgc
     1981 cggcgggtcg ttgtcatcag acccggttgg caccacgcca accacgtcga acccactcgt
     2041 gatcaccgtg accagcccct tggcagggac ggtcacgatc gacaagacgc ccccgaatac
     2101 gccggtcgtg ggctatcgac agcttggcct tggcgccaca atctcagcgc cggtgacatc
     2161 gaccgccgcg ccgt
//
LOCUS       DASWVF010000674         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_299037, whole genome shotgun sequence.
ACCESSION   DASWVF010000674 DASWVF010000000
VERSION     DASWVF010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299037"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1287)
                     /locus_tag="VGD55_11880"
     CDS             complement(100..1287)
                     /locus_tag="VGD55_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0871091.1"
                     /translation="MNKTAIRLVAVAAVAGLGIAGCSSKKASTAASSAPAAATTSAAP
                     AATSSAAPAATSSAAPPASSAPAASGTVGVILPDTQSSTRWETADRPALVKAFAAAGI
                     KADIQNALGDNAKFQSIAQSMIQEGVKVLIITSIDPATSIQVETEAKSANIPVIDYDR
                     LVLGGSAAYYASYDGVQVGVEQGQCLVDAMAKIGKPTGSIIMIDGSPTDNNATLFGQG
                     AHSVIDKSTLKKVSEQAIPNWDNTQAGKTFQQLLTANGNKIDGVYVMNDGMAGPVITV
                     LKADGLNGKVPVTGQDATSAGLQAILSGDQTCTIYKDATAEATAAATLAVDLIQGKTP
                     DGLTTSNDPQGKRSVPSILLKPELIDITTVGDPIKSGAVPYASVCTGAFVALCTKAGI
                     AAS"
     gene            complement(1470..2417)
                     /locus_tag="VGD55_11885"
     CDS             complement(1470..2417)
                     /locus_tag="VGD55_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0871092.1"
                     /translation="MRRLALASLVANVGIVATGGLVRLTNSGLGCPTWPRCENSDFVP
                     TNALSWHGAVEFGNRMLTGVVLAVVAAVFIAAVRLRPANAEIRRWAWVTLLGVPAQAV
                     MGGLVTLSKLNPWVVAPHFLLSMVLIAASTVLWWRARAPRDVGPPLPRLVRRMGLAVW
                     AVTYLVFVIGTAVTGSGPHAGNATAIRLPFRTEAVSQLHADVVMLLVGLAISLAVYVT
                     AAGAPAAVRTTTRILVGVLAAQATIGFAQYFSGLPIGLVELHVTGAAVLAAAATAVVV
                     ALHDTTPTRAPHEGHEGHEGREGPAGSESHERQDLRNTV"
BASE COUNT          434 a          838 c          901 g          449 t
ORIGIN      
        1 gtcggcccag atctcatgac agtcacagca cgcaaacaag gaaccggtgg cgtggccggg
       61 ttgaccggcc acgccaccgg acgagctccg ggatggggct taggaagccg cgatcccagc
      121 cttggtgcag agtgcgacga acgcgccggt gcagaccgat gcgtacggca ctgcgccgga
      181 cttgatcggg tcgccgacgg tggtgatgtc gatcagttca ggcttgagca ggatcgaagg
      241 aacgctgcgc ttgccctgcg ggtcgttcga cgtcgtcaag ccatccgggg tcttgccctg
      301 gatcaggtcg acggccagcg ttgctgcggc agttgcctca gcggtcgcgt ccttgtagat
      361 ggtgcaggtc tggtcgcccg acaggatggc ctgcaagcca gctgatgttg cgtcctgacc
      421 ggtcaccggc accttgccgt tgagcccgtc ggccttgagg acggtgatga ccggaccggc
      481 catgccgtcg ttcatgacat acacgccatc gatcttgttg ccgttggccg tgagcaactg
      541 ctggaaggtc ttgccggcct gggtgttgtc ccagttcggg atggcctgct cggaaacctt
      601 cttcaaggtg ctcttgtcga tgaccgagtg cgcgccctga ccgaacagcg tcgcgttgtt
      661 gtcggtcggt gaaccgtcga tcatgatgat cgatccggtc ggcttgccga tcttcgccat
      721 cgcgtcaacg aggcactgac cctgctcgac gccgacctga acgccgtcgt acgaggcgta
      781 gtaggcagca ctgccgccga gaaccaagcg gtcgtagtcg atgacgggga tgttcgcgga
      841 cttcgcttca gtctcaacct ggatcgacgt tgcagggtcg atcgacgtga tgatgaggac
      901 cttgacgcct tcctggatca tgctctgcgc gatgctctgg aacttggcgt tgtcgccgag
      961 cgcgttctgg atgtcggcct tgatgccggc agcggcaaac gccttgacga gggccgggcg
     1021 gtcagcggtc tcccaacggg tggacgactg agtgtcgggc aggatcacgc cgacggtgcc
     1081 gctggctgca ggggcgcttg acgcgggcgg ggccgccgag ctggtggcag caggcgctgc
     1141 tgagctggtg gcagcaggcg ctgcgctcgt ggtggcggcg gcaggagcgc tggacgccgc
     1201 ggtggatgct ttcttgctgc tacagccggc aatgccgagc ccggctaccg ccgcgacggc
     1261 aacgagccga atcgcagtct tgttcatgca cggtcccctt ttgcttcctg ggcgcaggac
     1321 gtggtgccct gcaggactgc ccagttgttg tcgcgcacaa catatgcacc gcgagcgcgg
     1381 aacgtaaggg ggaacggtgt catcgcccac gttcggaatg gatctgttac ataggcgtga
     1441 ccctgcgcgg tcagtcgaat accgacgttt tagacggtat tgcggaggtc ttgcctctcg
     1501 tgactctcag accccgcagg cccctcgcgc ccctcatgcc cctcatgccc ctcatgcggg
     1561 gccctcgtgg gggttgtgtc gtgcaatgcc acaaccacgg ccgtcgcagc cgctgcgagt
     1621 accgcggcgc ccgtgacgtg tagctccacg agcccgatag gcagcccgct gaagtattga
     1681 gcgaatccga tcgtcgcttg ggcggcaaga accccgacga ggatgcgagt ggtggtgcgc
     1741 accgcggccg gcgcacccgc tgctgtgacg tagaccgcca gcgatatggc caagccgacg
     1801 agcagcatca ccacgtcggc atggagctgg ctcaccgcct cggtccggaa cggcagccgg
     1861 atcgcggtgg cgtttcctgc gtgcggtccg ctgccggtga ctgcggttcc aatcacgaac
     1921 accaagtacg tgacagccca gacggcgaga cccatgcgcc gcacgaggcg gggcagtggc
     1981 gggccgacgt cgcgtggcgc gcgagcacgc caccacaaca cggtgcttgc cgcgatcaac
     2041 accatcgata gcaagaagtg gggcgcgacc acccacgggt tgagcttcga cagcgtgacc
     2101 aggccaccca tcacggcctg ggctgggacg ccgagaagcg tgacccaggc ccaacggcgg
     2161 atctcagcgt tggcaggccg cagccgcaca gcggcgatga agaccgcggc aaccacggcg
     2221 agaacgacgc cggtgagcat gcgattgccg aattcgacgg caccgtgcca tgacagcgcg
     2281 ttcgtgggca cgaagtcgct gttctcgcag cgcggccagg ttgggcagcc aaggcctgag
     2341 ttcgtgagcc tgacgagccc gccggtggcc acgatgccca cgttggcgac cagcgaggcg
     2401 agcgcgaggc ggcgcatggt gaccgaggac ggccgccagt ctcgccagcg ccgccacctc
     2461 ccatgccgcc acttcccatg ccgccactgc ccgccggtgg ccggcgaatc cggcgactga
     2521 cgggtggtcg attgggccga aggggctgtg gacgctgcca ttgcgcgacg atcgtacggc
     2581 tggtagccgc tgccgcagcc cgatccaccc gccgtccagc tt
//
LOCUS       DASWVF010000675         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_44264, whole genome shotgun sequence.
ACCESSION   DASWVF010000675 DASWVF010000000
VERSION     DASWVF010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44264"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VGD55_11890"
     CDS             <1..843
                     /locus_tag="VGD55_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871093.1"
                     /translation="ADGGRTWTTAPEGSGHGDHAGDADWIQLLSPTVAFDENIEATAS
                     NMSLRVTTNAGASWQTLYTGPPAASTPTPSAYEAPMLFVSQLRGFAVSGIPPAESQID
                     QGFFETSDGGVSWAVEKPPPARSTTCPSDNLGTVECLFSLPSFSDANHAVLASEVVDG
                     AHATVGFDVTGDGGSTWRQRTTVDVPIPLVPAGSYPGTYALVATPSSRDWWIVSATGT
                     GVTTRITSNAGQDWAITNSPRVLGSPGKLEALDATHALLMTQLITSDGGTSALYVTSD
                     GGRT"
     gene            897..1196
                     /locus_tag="VGD55_11895"
     CDS             897..1196
                     /locus_tag="VGD55_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871094.1"
                     /translation="MSLVNDFLTFIGVYNGNSRWYLFWGGFGSCLSELAILAVVWRKI
                     NCHAKGCYRVGLHHVDGTPYITCGKHHPAHPGSGACTAEQIAEAHADAQQAAPAA"
     gene            1305..>1647
                     /locus_tag="VGD55_11900"
     CDS             1305..>1647
                     /locus_tag="VGD55_11900"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0871095.1"
                     /translation="MPTRITPSLWFDTEAEDAAIFYTSVFKNSRVINVNRYTDAGPRP
                     AGTVMTVDFELDGTRFVGINGGPQFTFDEAVSFQISCEDQGEVDYFWERLSEGGSEGP
                     CGWLKDKFGLSW"
BASE COUNT          306 a          547 c          518 g          276 t
ORIGIN      
        1 gcagacggtg gcaggacttg gaccaccgcg cccgaagggt ccggccacgg cgaccatgct
       61 ggcgacgccg attggatcca gctgctgagc ccgaccgtcg ctttcgacga gaacatcgag
      121 gctacggcgt ccaacatgtc cctgcgggtc accacgaacg caggggcgtc gtggcagacc
      181 ctgtacacgg gcccacccgc agcttcgacc ccgaccccga gcgcatacga agcgccgatg
      241 ctcttcgtct cccaactccg cgggtttgcc gtgtctggca tcccgcccgc cgagagccag
      301 atcgaccaag ggttcttcga aaccagcgac ggcggggtct cgtgggcggt tgagaaaccc
      361 ccgcccgcac ggtcgacgac gtgtccgtcg gacaatctcg ggacggtcga gtgcctgttc
      421 tcgctgccat ccttcagcga cgcaaatcac gcggtgcttg caagcgaggt cgtcgacggc
      481 gcgcacgcca ccgtgggatt tgacgtcacc ggcgacggcg ggtcgacctg gcgacagaga
      541 accaccgtcg acgtcccgat accgctcgtt cctgcgggca gctatccggg gacctacgcg
      601 ctcgtcgcga caccgtcgag tcgcgactgg tggatcgtgt ctgcgaccgg cacgggcgtg
      661 accactcgaa tcacttcgaa cgccgggcaa gactgggcga ttaccaacag ccctcgggta
      721 ctcgggtcgc ccggcaagct cgaggcgctc gacgctacgc acgctctgct gatgacgcaa
      781 ctcatcacgt cggacggcgg aacgagcgct ctctacgtca cgagcgatgg cgggcgcacc
      841 tgaaaaacgc tctttggtag ctgagcaatt gggcgtcagc gcgataactt cgagccatga
      901 gcctcgtgaa cgatttcctc acattcatcg gtgtctacaa cggcaacagt cggtggtacc
      961 tgttctgggg cggcttcggc agttgcctca gcgaactcgc gatcctcgcg gtcgtctggc
     1021 gcaagatcaa ctgccacgca aagggctgct accgagtggg actgcaccac gtcgatggca
     1081 cgccgtacat cacctgcggc aagcaccatc cggcgcatcc gggcagcggc gcgtgcacgg
     1141 ccgagcagat cgccgaggcg catgccgacg ctcagcaggc cgctcctgct gcgtaaagcc
     1201 aggccaagcc ggtcggacgg cgatgagttc cgtcggcgcg gaacgtcggt atgggtgcac
     1261 gatgggttga agaaccgttc cgccgtgacc gaaaggacgc tgcgatgcca accagaatca
     1321 ccccgagcct atggttcgac accgaggccg aggacgccgc gattttctac acttccgtgt
     1381 tcaagaactc gcgtgtcatc aacgtcaacc gatacaccga cgccggtccg cgccctgccg
     1441 gcaccgtgat gactgtcgat ttcgagctgg acgggacccg attcgttggc atcaacggtg
     1501 gcccgcaatt cacgttcgac gaggccgtct cgttccagat cagctgcgaa gaccagggcg
     1561 aggtcgacta cttctgggaa cgactctccg agggtggctc cgagggcccc tgcggctggc
     1621 tcaaagacaa gttcggtctc tcgtggc
//
LOCUS       DASWVF010000676         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_197499, whole genome shotgun sequence.
ACCESSION   DASWVF010000676 DASWVF010000000
VERSION     DASWVF010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197499"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1538
                     /locus_tag="VGD55_11905"
     CDS             291..1538
                     /locus_tag="VGD55_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0871096.1"
                     /translation="MSGGSRLDPYVDRYAARTHGMTTSEIRALFAVANRPEIIGMGGG
                     SPCVSALPLEVIGEMTAKLITDHGAQALQYMGGQGDERLRELICGVMALEGISASADD
                     VIVTVGSQQALDVVSRIFLDPGDVVIAEAPSYVGALGTFTQYQADVVHVTMDDEGLVP
                     DALRAAIADIARSGRRAKFVYTVPNYHNPAGITLIEQRRDEIVAICADAGLLILEDNP
                     YGLLGFDGPPLPALRARVADGIIYLGSFSKTFAAGVRVGWVLAPPGVKEKLIMASEAT
                     TLCPVALSQYVVAEFLAHHPWQQQVKVFRELYRTRRDACLEALEASFPVGSSWTRPAG
                     GFYVWVTLPEGIDAKAMLPRAIQGRVAYVPGVGFYSDGQGRRNLRLAYSLPEPDRIRE
                     GIRRLAGVLAEELDIVATFGGGA"
     gene            1535..2509
                     /locus_tag="VGD55_11910"
     CDS             1535..2509
                     /locus_tag="VGD55_11910"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0871097.1"
                     /translation="MSDLGHVLVLAGGLSYEREVSLSSGRRVTDALASLGVEVELVDI
                     DAQLLPRLLQDPPSAVFLAVHGIEGEDGGLRDALDLSDVPYVGATGAAARLAFDKPVA
                     KAIVGRAGVHTPASVALQHAAFRDLGGAALLERVVDRLGLPLVVKPARGGSALGVTVV
                     RDAHELPAAMVGCYSYDGVALLERHIVGVELAVSVVDDGSGPVALPAVEIEALSGTFD
                     YASRYTAGMTEYHVPARLSPELARAAGAVAVLAHQALGLRDLSRTDLIVSADGTIEYL
                     ETNVAPGMTTTSLLPLAAAAAGLELGALCRDLLHRAAVRGFSAPGAPL"
     gene            2546..>2741
                     /locus_tag="VGD55_11915"
     CDS             2546..>2741
                     /locus_tag="VGD55_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0871098.1"
                     /translation="MTTLENRPNTALLVVDVQNGVVGEAYERDAVVANIRSLVAKARL
                     SHVPVVWVQHSDDEIINGSND"
BASE COUNT          444 a          930 c          875 g          492 t
ORIGIN      
        1 tccttttcca acgccacgtc gcccgactcc tcggctcgcg cgcgactgac cggatccagt
       61 tcccagaagg cgcagcgcct gcatcgaacc ggcagatcgt caaggttgtc gagggtcaac
      121 ccgaccacgc gtcgcgacat cgcacctcca cgggcgcata gggccccgaa cagccccgaa
      181 ccgcctcgtc agcgcaatgc ttctcggcga gtcgggcgga ccgctaaata ggcggacccg
      241 aatcaccggg acggtagcct tgagctctca aggaggacgc tttcgtggac atgtcaggtg
      301 ggtcgcggct cgacccgtat gtcgatcgtt atgccgcccg cacacacggc atgacgacgt
      361 cggagatccg cgcgctgttc gccgtcgcta accggccgga gattatcggc atgggcggcg
      421 gctcgccgtg cgtcagcgcg ctcccgctcg aggtcatcgg tgagatgacc gcgaagctca
      481 tcaccgacca cggcgcacag gcccttcagt acatgggtgg ccagggcgac gagcgcctgc
      541 gcgaactgat ctgcggagtc atggcactcg aaggaatctc agcgtccgct gacgacgtca
      601 tcgtcaccgt gggttcacag caggccctcg acgtggtgtc gcgcatcttc ctcgatccgg
      661 gcgatgtcgt catcgccgaa gcaccctcct acgtcggcgc cctcggaaca ttcactcaat
      721 atcaggcaga cgtcgtacat gtgacgatgg acgacgaggg gctggtgccc gacgctttgc
      781 gagcggctat tgccgatatc gcccgttctg ggcgtcgcgc aaaattcgtc tatacagtgc
      841 cgaattacca caatccagca ggtatcaccc tcatcgaaca gcggcgcgac gagattgttg
      901 ccatttgtgc ggacgcgggc ctgctgattc tcgaggacaa cccctacgga ttgctcggct
      961 tcgacggtcc cccgcttccc gctctccggg cgcgggtcgc cgacgggatc atctacctcg
     1021 gctcgttctc caagaccttc gccgcgggtg tgcgagtcgg ctgggtactg gccccgccag
     1081 gggtgaaaga gaagctcatc atggcgagcg aggccacgac gttgtgcccg gtcgcgctgt
     1141 cgcaatacgt ggtcgccgag ttcctggccc atcatccgtg gcaacagcag gtgaaggtct
     1201 ttcgcgagtt gtaccgaacc cggcgcgacg catgtctcga agcgctcgaa gcgtcgttcc
     1261 ctgtgggttc ttcctggacc cggcctgctg gcggcttcta cgtctgggtc accctgccag
     1321 agggcatcga cgccaaagcg atgctcccgc gagcgatcca gggccgcgtt gcctacgtgc
     1381 cgggcgtcgg cttttactcc gacgggcagg gacgccgaaa cttgcgcctt gcctatagcc
     1441 tccccgagcc cgatcgcatc cgcgagggca tccgcaggct cgccggcgtg ctggccgagg
     1501 agctcgacat cgtcgcaact ttcggtggcg gcgcttgagc gacctcggac acgtgcttgt
     1561 tctcgccggc gggctctcgt acgagcgaga agtgtcgctg tcgtcagggc gtcgggtcac
     1621 agacgcgctg gcgagccttg gggtcgaggt cgaactggtc gacatcgatg cgcagctact
     1681 gccgcggttg ttgcaagacc cgccgtccgc cgttttcctt gctgtgcacg gaattgaagg
     1741 cgaagacggc ggtctgcgcg atgcccttga tctcagtgac gtgccgtatg tcggggctac
     1801 cggcgcggcc gcaaggttgg cgttcgacaa accggtggcc aaagcgatcg tgggccgcgc
     1861 cggcgtgcac accccggcgt ccgtcgcctt gcagcatgca gcttttcgtg acctcggcgg
     1921 cgcggcactt ctcgagcgcg tcgtcgaccg gctcgggcta ccgctcgtcg tcaagcccgc
     1981 acgtggaggc tcggcgctcg gggtcaccgt cgtgcgcgat gcccacgagc tccccgcggc
     2041 gatggtcggt tgttactcct acgacggcgt cgcgttgctc gagcgacaca ttgtcggcgt
     2101 cgaactcgcg gtcagcgtcg ttgacgacgg gagtggtccc gttgcactgc ccgccgtcga
     2161 gatcgaggca ctcagtggca cgttcgacta cgcctcgcgc tacaccgccg gcatgaccga
     2221 ataccacgtg cccgcccggc tgagccccga gcttgcgcgg gctgcaggtg cggtcgcagt
     2281 gttggcccac caggctctcg gcctgcgcga cctttcgcgc actgacctaa tcgtgtcggc
     2341 tgacgggacg atcgagtacc tcgagaccaa cgtcgcgccc ggcatgacga ccacgagcct
     2401 gctgccccta gcggcagcag cagccggcct cgagctcggt gctctttgcc gcgacctgct
     2461 ccaccgcgcc gctgtccgtg gattctcggc gccaggcgcg ccgctctaac agccctggaa
     2521 cgggccacac cgacaaggac tcgccttgac cacactggag aaccgcccga acacggcact
     2581 gctcgtcgtc gacgtgcaga acggcgtcgt cggcgaagct tacgagcgcg atgcggtcgt
     2641 cgcgaacatt cgcagcctgg tcgcgaaggc gcgactgtcg cacgtcccgg tcgtctgggt
     2701 tcaacactcc gacgacgaaa tcatcaacgg cagcaacgac t
//
LOCUS       DASWVF010000677         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_477885, whole genome shotgun sequence.
ACCESSION   DASWVF010000677 DASWVF010000000
VERSION     DASWVF010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477885"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..>1766
                     /locus_tag="VGD55_11920"
     CDS             126..>1766
                     /locus_tag="VGD55_11920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0871099.1"
                     /translation="MARELHASDPAVERPYPVGPELEQVVLRNMLNDAAEAIYFKDLQ
                     SRFIAVSRALATLHGREPEELLGLTDFDLFTPEHAGAAYADEQRVIATGVPLLNKEEK
                     ETWPDRGDTWVTSNKRPLRDLDGVIIGTFGLSRDITRRVTAEQDATRAADELALTHAE
                     LGRVEAQLRTVLDTSSDGIALYDTQLRYQYLNAAARRAAEDPYWDFLGRTDRELGRDE
                     AFLAVWEASLKSVLSTGENRSVDFSLGSGRDLRWFAAQLSAQRETQHGPPIGVVASTR
                     EVTELKRAQSKLAHQAVHDPLTGLANRVLLTDRLTRALLRMERQPGRIALLFVDLDHF
                     KDVNDAHGHEAGDRILVEVGERLTEIARRIDTVARFGGDEFVLLCDKLNTDDDVRVVA
                     DRVVRGIAEPFVVNGGAALHVTASVGVVMTCDPYATSESLVRDADAAMYQAKERGRNH
                     FQFFDAGIRDLAAAKNATENDLSHALERGQFRLEYQPVFNLSGRRYIVGAEALIRWDH
                     PDRGTVPPTEFIGIAESRGLIVPIGTWVLNEACRQLVEW"
BASE COUNT          308 a          585 c          582 g          291 t
ORIGIN      
        1 gaccccagcc ccccagaagg cctgagcggg gaatttgcct tggattggca cgtgcgtggc
       61 cgatggggag cggataaatg acctagtccg gcccaagggc cgacccaacg gacgcgaggt
      121 gacgcatggc gcgcgagctc cacgcctccg accctgcggt cgagcgtccc tatccggtcg
      181 gacccgagct cgagcaggtc gtcctgcgca acatgctcaa cgacgctgcc gaggcgatct
      241 acttcaagga tctccagagc aggttcatcg cagtcagccg cgcgcttgcc acgttgcatg
      301 gcagggagcc cgaggagctc ctcggcctta ccgacttcga cctcttcacc cctgagcacg
      361 cgggcgccgc atacgccgac gagcagcggg tcatcgccac cggtgtgccg ttgctcaaca
      421 aggaggagaa ggaaacctgg cccgaccggg gcgatacctg ggtcacgagc aacaagcgtc
      481 cgctgcgcga tctcgacggg gtgatcatcg gtaccttcgg gctctcccgc gacatcaccc
      541 gtcgggtgac cgccgagcaa gacgcgacca gagccgccga cgagctagcc ctcacccatg
      601 cggagctggg gcgcgtcgag gcgcagctgc ggaccgtcct cgacacgtcg tcagacggca
      661 tcgcgctcta cgacacgcag ttgcgctatc agtacttgaa cgcggcggcg cgacgcgctg
      721 cggaggatcc gtactgggac ttcctcgggc gtaccgaccg cgagctcggt cgtgacgaag
      781 ccttcctggc ggtgtgggaa gcgagcctga agagcgtgct ctcgaccggc gaaaaccgct
      841 ccgtcgactt ttcgcttggc agcggtcgcg acctgcggtg gttcgcagcc cagctgtcgg
      901 cccaacgcga aacccagcac ggaccgccga tcggggtggt cgcgtcgacc cgtgaggtca
      961 ccgaactcaa gcgtgcgcag agcaagctcg cgcaccaggc ggtgcatgat ccgctcaccg
     1021 ggctggccaa ccgggtgctg ctgaccgacc ggctgacccg cgcactgctg cggatggagc
     1081 gccaacccgg ccgcatcgcc ctgctgttcg tcgaccttga ccacttcaag gacgtcaacg
     1141 acgcgcacgg gcacgaggcc ggtgatcgca ttctcgtcga agtcggcgag cggctcaccg
     1201 agatcgcccg ccgcatcgac accgttgcgc gctttggcgg cgacgagttc gtgctgctgt
     1261 gcgacaaact caacacggac gacgatgtgc gggtcgtcgc cgatcgcgtc gtacgtggta
     1321 tcgccgagcc gttcgttgtc aacggcgggg cggcgttgca tgtgacggca agcgtcgggg
     1381 ttgtgatgac ctgcgatccg tacgcgacgt cggagagtct cgttcgtgac gcggacgccg
     1441 cgatgtacca ggcaaaggaa cgcggccgca atcatttcca gttcttcgac gccggcattc
     1501 gagatcttgc tgccgcgaag aacgcgaccg agaacgatct gtctcatgcg ctggaacgcg
     1561 ggcagttcag gctcgagtac cagccggttt tcaatctctc gggtcgccgc tacatcgtcg
     1621 gcgccgaagc gctgattcgc tgggaccacc ccgaccgcgg cactgttcct cctacggagt
     1681 tcatcggtat tgccgagagc cgcgggctga tcgtgccgat cggcacctgg gtgctgaacg
     1741 aagcgtgccg tcagcttgtc gagtgg
//
LOCUS       DASWVF010000678         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_95517, whole genome shotgun sequence.
ACCESSION   DASWVF010000678 DASWVF010000000
VERSION     DASWVF010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95517"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1743)
                     /locus_tag="VGD55_11925"
     CDS             complement(67..1743)
                     /locus_tag="VGD55_11925"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEY0871100.1"
                     /translation="MTPVRSARAPAIGVAAATVVLYAILAVWACLTPPFRAPDEPQHV
                     STVLRLAYSHTYPAAGHAQLYPAVKGAEPVLGFRIVGDFLDTSPEKAAPASERGPQTF
                     RQLAAQAGPYTLKDFDQMTQHPPGYYAVMALPTRLFNLIDDTPRDAIVIMRLLSALLL
                     IPLPYLAFRLTRALGGGTIASMTAAFLPAGLPQLTHIGGAVNNDALIIPLAALLVVEA
                     VELAKFGATMKRAIWFGITYAAALWTKGMALPLLVLPIGAYLLAARREGLRQVLKPLV
                     ATAAISCIGFSWWIANVVRFHTLQPQGYPAAYLAQLPHGNATFSAWFSGFFDSMTKSF
                     WLDAGWLEMKPTRWAYVSASIVLLLLIVIAFIRSRGNRGLFVLAQIGWIAVMASIFQQ
                     SHAQFVDRSTLAGSQGRYLFTGVTAMAAAIVLALRPQEHQDHQAGRRVAGHGSAATRR
                     PTATRRAGSESRVPLAPLVPLAVVFVAFYGLARGLHHFYVGSSWSARLHVMSTWSPLR
                     VREIAVIAAVAVIAALVGVVALWASRSGGPEPAEPAEGSSRPGPQAQVAA"
     gene            complement(1740..>2328)
                     /locus_tag="VGD55_11930"
     CDS             complement(1740..>2328)
                     /locus_tag="VGD55_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY0871101.1"
                     /translation="VHVSTDYVFAGAATAPYDEGAQTDPRSAYGRTKLEGERAVLREL
                     PDAYIVRTAWVYGAVGNNFVKTMAKLERDRETVSVVDDQRGSPTWSYDLAQGLVALGS
                     SAAPGGIYHCTNSGETTWFGFTRAIFEELGADPDRVLPTTTAAFPRPAPRPAYSVLGN
                     RRWLEAGLEPLPHWRDALHEAFARSGPQLRAQSSS"
BASE COUNT          437 a          734 c          808 g          349 t
ORIGIN      
        1 ctgccccggg actaggtccg gagccgcctc cgggcgtccg ccgcggaggt tatgtgcatc
       61 ggcccgtcag gccgcgacct gagcctgcgg accaggccgg ctggagcctt cggcgggctc
      121 agcgggctct gggccgcccg atcgcgaggc ccacaacgcc acgaccccga ccagcgcagc
      181 gatgaccgcc acggcggcga taaccgcgat ttcccgcact cgaagcgggc tccaggtgct
      241 catcacatgc agccgagccg accaactcga tccgacgtag aagtggtgca gcccacgggc
      301 gagcccgtag aaggcgacga aaacgaccgc cagcgggacg agcggagcca gcgggacacg
      361 gctctcgcta ccggcacggc gagtagctgt gggacggcgg gtggcggccg aaccgtggcc
      421 agcaacccgg cgtccggcct gatggtcttg atgttcttgc ggacgcaacg cgagaacaat
      481 cgcggcggcc atcgcggtga cgccggtgaa caggtagcgg ccctgcgagc cggcaagcgt
      541 cgagcggtcg acgaactgcg catgcgactg ctggaagatc gaggccatca ccgcgatcca
      601 gccgatttgg gccagcacga acagcccgcg gttaccacga ctacggatga aggcgatgac
      661 gatgagcagc agcaagacga tgctcgcgga gacgtaggcc catcgcgttg gcttcatttc
      721 aagccagcct gcatcgagcc agaacgactt cgtcatcgag tcgaagaagc cactgaacca
      781 cgcgctgaag gtcgcgtttc cgtggggcag ctgtgcgagg taagcggccg gatatccctg
      841 tggttgcagg gtgtggaacc taacgacgtt cgcgatccac cagctgaatc cgatgcagct
      901 gatcgcagca gtcgcgacaa gtggcttgag aacctgccgc aagccctcac ggcgtgcggc
      961 gagcaggtac gcaccgatcg ggagtacaag caggggcaac gccatgcctt tggtccacag
     1021 cgcggcggca taggtgatgc cgaaccagat tgcgcgcttc atcgtcgcgc cgaacttcgc
     1081 caactcgacg gcttcgacga caagcagagc agcgagcggg atgatgagtg cgtcgttgtt
     1141 gacagcaccg ccgatgtggg tgagttgcgg caacccggcc ggcagaaacg ccgccgtcat
     1201 actggcgatc gtgccgccgc caagagcgcg cgtcagccgg aaagccaggt acggtaaggg
     1261 aatcagcaac aacgccgaga gcaaccgcat gatgacgatc gcgtcgcggg gcgtgtcatc
     1321 gatcagattg aagaggcgcg tcggcagcgc catgaccgcg tagtaacccg gcgggtgctg
     1381 ggtcatctgg tcgaagtcct tgagcgtgta cgggcccgcc tgcgctgcta gctggcgaaa
     1441 tgtttgcggg ccgcgctcac tcgctggcgc cgccttttcc ggtgaggtat cgaggaagtc
     1501 accgacgatg cgaaatccga gaaccggctc ggcgcctttg acggctgggt aaagctgcgc
     1561 gtgaccggct gccggatacg tgtgcgaata ggcaagacgg agaaccgtcg acacgtgctg
     1621 cggttcgtca ggtgcgcgaa acggcggggt gaggcaggcc cagacggcga ggatcgcgta
     1681 cagcacaacg gtcgcggcgg cgaccccgat cgcgggtgcc cgggctgacc gcacgggtgt
     1741 cacgacgacg attgtgcacg cagttgcggg ccggaccgtg caaatgcctc gtgcagcgcg
     1801 tcacgccaat gcggcaacgg ctcgagccct gcttcgagcc agcgccggtt gccaagtacc
     1861 gaatatgccg gtcgcggtgc aggtcgcgga aaggcagccg tggtcgtcgg aagtacccgg
     1921 tcggggtcgg cgccaagttc ttcgaagatg gcccgggtga acccgaacca tgtcgtctcg
     1981 ccgctgttgg tgcagtggta gatgccaccc ggtgcggctg acgatccgag tgcgacaagt
     2041 ccttgggcca ggtcgtacga ccacgtcggc gagccgcgct ggtcgtcgac gacggagacc
     2101 gtctcgcgat cgcgctcgag cttcgccatg gtcttcacga agttgttgcc gaccgcgccg
     2161 tacacccacg cggtgcgcac gatgtaggcg tcgggaagtt cgcgcagcac agcccgctcg
     2221 ccttcgagct tcgtccgccc gtaggccgac cgaggatcgg tctgcgcccc ctcgtcgtac
     2281 ggcgccgtcg cagcgccggc gaacacatag tcggtggaga cgtgcacg
//
LOCUS       DASWVF010000679         9483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_223352, whole genome shotgun sequence.
ACCESSION   DASWVF010000679 DASWVF010000000
VERSION     DASWVF010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223352"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGD55_11935"
     CDS             <1..234
                     /locus_tag="VGD55_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0871102.1"
                     /translation="DPDTLMSRSQTEYNTPDALLDMIKEDLVAERVVIQAYTETIAWL
                     GHNDPTTRRIFEEILAEEEEHADDMLTLLQRNS"
     gene            complement(266..1159)
                     /locus_tag="VGD55_11940"
     CDS             complement(266..1159)
                     /locus_tag="VGD55_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0871103.1"
                     /translation="MTDDDWLADGFDACRPHLHAVAYRMLGSVPEAEDAVQESWFRLA
                     GNDATAINDLRGWLTTVVGRICLDMLRSRRVRQDGYFGVRLPNPIVEEDMGADPEGQV
                     ILADSVGMALLVVLETLAPAERLAFVLHDVFGVPFDQISEIIDRTPDATRQLASRARR
                     RVQQAPQPDADVPKQRRAVDAFVAAAREGDFDALMTILDPDVVFRADAGPDGPDTSGM
                     DVIASPFRRPLIGANAVARQVLRTAPRFISLVRPMLVNGSPGLLVGTPTAPMGAVAFT
                     VVKGRIVAIDLVVGAVTVRHS"
     gene            1331..2314
                     /locus_tag="VGD55_11945"
     CDS             1331..2314
                     /locus_tag="VGD55_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0871104.1"
                     /translation="MVTINDRPVIEAQELVKTYAGGIQAVRGVSFEVGAGEAFGLLGP
                     NGAGKSTTIGMLNSTVKPTSGRAFLGGIDVDRDPIGTRAISSVVFQDTVVDRPLTGRR
                     NLEVHLRLWGVAASTGKAALAELAKTVGIADILDRQVATYSGGQRRRLEIVRALLSAP
                     RVLFLDEPTVGLDTRIRHELFDVIATLRERTGVTIIMTTHYLDEAERLCDRLAVIDRG
                     SVVACDTPANLLALVGEEVLELRVNDAGKAASAVLDAGIPAEDVLIVGETVTISLRAL
                     SSNHILGVLAAMEVAVRSATTRRPTLDDVYLRLTGDLLLASSDAGNREDRS"
     gene            2311..3159
                     /locus_tag="VGD55_11950"
     CDS             2311..3159
                     /locus_tag="VGD55_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871105.1"
                     /translation="MTTATINAQALRVHRRSKSVNAIPTLTMRRLALTIRSPRSLAVP
                     LLAPLLFALVVAPALSNTVAAPGQRTASLTFFALATAGLLIPLNCMFSGLGVVVDRAQ
                     GAMRELLVAPIRRVDIVLGNLLAAMVITTLQLSVLIGAAAVRGAAFETGRHVLWFLAA
                     AGLFCVVIYGLAEILATRLTSPEEYTAALPAVAIVPFFFAGSLYPITALPHWLEDVAR
                     VLPLTHTLALFRYGLTSNGATALHSIWGMRSDTTMAALSLGVVALYASVIIIVAIRVF
                     TKAGKN"
     gene            3219..3740
                     /locus_tag="VGD55_11955"
     CDS             3219..3740
                     /locus_tag="VGD55_11955"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0871106.1"
                     /translation="MTEEIRGVPTSAARIRLSRIVDALDSNLQGNVHGGAIMRLVDEA
                     AGVVASRHSGGRAVTAVVDELQFLVPVHVGDIVTCDAQVNWTGRTSCEVGVRVTAQPW
                     NQAGDVGRHVASAYLVFVAIDDDEHPRTIPPVMPQNDEDRRRFREAEIRRGTRLARRE
                     AILRSRERGSAQP"
     gene            complement(3731..5314)
                     /locus_tag="VGD55_11960"
     CDS             complement(3731..5314)
                     /locus_tag="VGD55_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY0871107.1"
                     /translation="MAPPTGRPKVLDPAGEQAAYEHAQRRARIIGRLIVGFFAVVVLI
                     GGIVGVAFKEIFGKVHFQKSLSHVLVASARPQPAAKPNTATGGGAAGGAGGGTAKNIL
                     ILGVDSRLGENSTFQVKGAPAQTESLSDTAILAHLAANGKDVTLVSIPRDSVVQIPAC
                     EKVDSDGNPILDKNGQLQYSKPTKALFNEAIELGGPYCSAKTLESLSGVRIDNFLEID
                     FTGVVKMSKAIGGVPLNICSPIHDSNTGLNLPAGPVNLEGDQALAFVRARYGLTGGDD
                     LHRIQRQQQFMASMVRKALSSANFFNPATMYSFYSAVASSLSTDMTSSQLLSLGINYR
                     HTNTSNIVFVTVPTYPAPKGDPFYEHLYWSEDEATALFADIHDDRAIPVTSGSNVANI
                     TLPRSAVSVEVLNGTSTDGLARQVRDELSAAGFDVVSIGTASNSPVAKTTVAYQASHT
                     TSMQTLVGALSVTPQEVLDSSSGSNIVLTLGQDWGGLATASPSASAPASAPASSPATA
                     APIPNLKATNAGNTSCVQG"
     gene            complement(5437..7002)
                     /locus_tag="VGD55_11965"
     CDS             complement(5437..7002)
                     /locus_tag="VGD55_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY0871108.1"
                     /translation="MGHPGLPPERPGHPGFPGEHLALPPELNPRGTRRPAKSGKPRKP
                     SRPTKRGTWRSPLGITAAVLAVCVLAASGLLYTRYLHYDHNLTRAAGVIKPGGAAATD
                     GAENVLLVGSDSRTGAGDQFAQAPKGQTAVEGARSDTVILAHLAKGHQAATLVSLPRD
                     SWVTIPAYTDAKGVVHPAHMDKLNAAFSLGGAALLVSTVQQLTEIHIDHYVEIDFAGF
                     QSMVDSLGGVDVCLSKSAHDVQTGLNLSAGVHHLDGKTALAFVRQRYGLPLGDIDRIK
                     RQQQFLASMVRKVESAGTLTNPLKLNDFLDALTKSISVDSGMSAADLAKLALKLKGLG
                     TGNIVLTTMPLSGFSTQNGVDVDDVDVPKATALFSSLAIDAPVAATTSAAAPAAPAAA
                     LTVPASAVHVQVFNGGGVTGLARRAASDLSKLGFQLVGTPSDRINHASGSVIAYGPTQ
                     AEAARTLQAAIPGSTLQANPQLDTTLELTVGSSYAGAHTPGASASPSTTATSPANAET
                     TGGTTAANASCTD"
     gene            complement(7447..7662)
                     /locus_tag="VGD55_11970"
     CDS             complement(7447..7662)
                     /locus_tag="VGD55_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871109.1"
                     /translation="MSGTPAAPTYLVGLSKGTGTLLHEIGALLLRIGALLLDTGALPF
                     ETSALPLETSALPLETGPVTPELVLAR"
     gene            7661..7966
                     /locus_tag="VGD55_11975"
     CDS             7661..7966
                     /locus_tag="VGD55_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871110.1"
                     /translation="MTAVLDWIRSGSTARGTMGLSGHPEPITEIPPGSYPLCISFVGD
                     SFTGRLEVKGQPVPISGQLTATGVDVEFDASPAMDPTLRFTSIGPGSYSYPPSHPAS"
     gene            8040..9104
                     /locus_tag="VGD55_11980"
     CDS             8040..9104
                     /locus_tag="VGD55_11980"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEY0871111.1"
                     /translation="MKALVLSGGAGTRLRPITHTSAKQLVPVANKPVLFYGLEAIRDA
                     GITDVGIIVGDTHAEIEAAVGDGSALGIKATYIPQDAPLGLAHCVLIARDFLGNDDFV
                     MYLGDNFIIGGITDLVDEFKRGGADAQILLTRVDNPQQFGIAELDDAGHIVRLVEKPA
                     EPKSDLALVGVYMFSPTIHEAVNAITPSRRGELEITDAIQWLVDHGKDVRPHLVTGYW
                     KDTGRLEDMLECNRKVLESVEPSCLGSVDDLSRIIGRVVIEEGASIVRSTVRGPAIIG
                     RDTRIVDSYVGPFTSIYHDCVIDSTEVEHSIVLESTTITGVSRIEDSLIGKEVEVSPS
                     TAMPRAHRLMLGDHSKVSLA"
     gene            9113..>9483
                     /locus_tag="VGD55_11985"
     CDS             9113..>9483
                     /locus_tag="VGD55_11985"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0871112.1"
                     /translation="MRASADPTSGRRIDADIVGVATLTISAAAVYTVFEIEQQRHFNT
                     AGYDLGIFDQAIHSYAHFHAPISLMKGVHNGFGTHFSELGDHFSPIIAAVAPVYRVFP
                     DAVTLLIVQGLLLAASVPSGWL"
BASE COUNT         1601 a         3061 c         3107 g         1714 t
ORIGIN      
        1 gaccccgaca cgctcatgtc ccgctcgcag acggagtaca acacccctga cgcgctgctc
       61 gacatgatca aagaggacct cgtcgccgag cgggtggtta tccaggctta caccgagacg
      121 atcgcgtggc tcggccacaa cgacccgacc actcgccgga tcttcgaaga gatcctggca
      181 gaggaggaag agcacgccga cgacatgctc accctgctcc agcgcaacag ctgaccctgg
      241 gccacacctg ggcggctgcg ttgtcctagc tgtggcgcac tgtcaccgca ccgaccacaa
      301 ggtcgatcgc cacgatccgg cctttcacga ccgtgaacgc aacggcgccc atcggcgcgg
      361 tgggcgttcc aaccaggagg cccggtgacc cattgaccaa catgggccgg accagcgaga
      421 tgaaacgtgg cgccgtgcgc agcacttgtc gggcgaccgc gttggcgccg atcaggggac
      481 gccggaacgg cgaagcgatg acgtccatac ccgacgtgtc gggaccatct ggtccggcat
      541 ctgcccggaa caccacatcg ggatcaagga tggtcatgag tgcgtcgaag tcgccctccc
      601 gcgcggcagc cacgaacgca tcaacggctc gtcgctgctt cgggacgtcg gcgtcgggtt
      661 gcggtgcttg ctgtaccctg cgccgcgccc tgcttgcgag ttggcgagtc gcgtcgggcg
      721 ttcggtcgat gatctccgag atttgatcga acgggacgcc gaaaacgtcg tggagaacga
      781 aagcgagccg ctccgcaggc gccagggttt ccaggacgac gagcaacgcc atgccgaccg
      841 aatcggcaag gatcacctgg ccttcaggat ccgcgcccat gtcttcctcg acgatcggat
      901 tcggcaaccg aacaccgaag tacccgtctt gcctaacgcg cctggaccgg agcatgtcga
      961 ggcagatgcg acccacgaca gtggtgagcc aaccgcgcag gtcattgatt gcagtggcgt
     1021 cgttgcccgc gagccgaaac cacgattcct gtaccgcgtc ctcggcttcc ggcacagaac
     1081 cgagcattcg gtacgcgacc gcatgtaggt gcgggcggca cgcatcgaag ccgtccgcca
     1141 gccagtcgtc gtccgtcata ggtgtgcctg gactttcttc cataaccaaa ggacgcgcgg
     1201 gcggcacgga atgtgacatg gcgccgcgcg tcacattcca ctccagcgcc acgtcttcat
     1261 taatagctgg tctccgacag cgggctgcgc ggcagccacc acgcaggcaa tcgagggaga
     1321 tatacgtgtg atggtcacca tcaacgacag acccgttatc gaggcgcagg agctggtcaa
     1381 gacctacgcc ggcggcatcc aagcggtccg gggagtcagt ttcgaggtcg gggccggcga
     1441 agcattcggt ctcctcgggc caaatggggc aggcaaatcg acgacaatcg ggatgctcaa
     1501 cagcaccgtc aagccgacat cggggcgggc tttcctgggc gggatcgacg tcgaccgtga
     1561 tccgattggc acccgcgcga tcagcagtgt ggtttttcag gacacggtcg tcgaccgtcc
     1621 gctgaccggt cggcgcaacc tggaggttca tctgcggctg tggggcgtgg ccgcatcgac
     1681 gggcaaggcc gcgctcgcgg agctggcgaa gaccgttggc atcgctgaca tccttgaccg
     1741 ccaagttgcc acttatagcg gtggccagcg caggcgactc gagattgtcc gggcgctgct
     1801 gtcagctccg cgggtgctgt tcctcgatga gccgaccgtc gggctcgaca cacgtatacg
     1861 tcacgagttg ttcgacgtca tcgcaactct gcgcgaaaga accggcgtca cgatcatcat
     1921 gaccacgcac tacctcgacg aggcggagcg actctgcgac cggctggctg tcatcgaccg
     1981 gggatcggtc gttgcgtgcg acacgccagc caacctcctc gcattggttg gcgaggaggt
     2041 cctcgagctc cgcgtgaacg acgcgggaaa ggcggcgtcg gcggttcttg acgcaggtat
     2101 cccagcggag gatgtcctga ttgtcggcga gaccgtgacg atcagcctgc gggcactgag
     2161 cagcaaccac atccttggcg tccttgctgc gatggaggtt gccgttcgtt cggccaccac
     2221 acgtcgtccg acattggacg acgtctacct gcgtctcact ggcgacctgc tactcgcgag
     2281 cagcgacgcc ggcaatcgag aggaccgatc atgacaaccg ccacgatcaa tgctcaagca
     2341 ctacgcgtgc atcgacgctc gaagagcgtt aacgcgatac cgacgctgac gatgcgccgt
     2401 ttagcattga cgatcagatc gccaaggtcg ctggcggtgc cgcttctcgc gccactgctg
     2461 ttcgcgctcg tcgttgcgcc cgcgctgtcg aatacagtcg cggcgccagg ccagcgaacg
     2521 gcgtctctga cattcttcgc tctggcaaca gcggggctcc tgatcccgct caactgcatg
     2581 ttctctggcc ttggtgttgt cgtcgacagg gcgcagggtg cgatgcgcga actgctcgtg
     2641 gcgccgatcc ggcgcgtgga catcgtgctt ggcaacctcc ttgccgccat ggtgatcacg
     2701 acactccaac tgagcgttct cattggtgcc gccgccgtgc ggggcgcagc gtttgagacc
     2761 ggtcggcacg tcctgtggtt cttggcggcg gcagggctct tctgcgtcgt gatctacgga
     2821 ctagcggaga tcctcgcgac gaggctcacg agccctgagg aatacacggc tgcgcttccg
     2881 gccgtcgcga ttgttccgtt cttcttcgcg gggtcgctgt atccgatcac agcactaccg
     2941 cattggttgg aggacgtcgc tcgcgtgctg ccgctgacac atacgctggc cttgttcagg
     3001 tacggattga cgtcgaacgg cgccacggcg ctgcatagca tctggggcat gcgcagcgac
     3061 acaaccatgg ctgcactgtc gctcggcgtg gtcgcgctgt acgcaagcgt gatcatcatc
     3121 gtggcgattc gggtcttcac gaaggcgggc aagaactagc tttcggcgag caggactccg
     3181 gccaccgagc atgcacgggg cggtgggatg atcaagccgt gaccgaggag attaggggcg
     3241 tgccgaccag cgccgcccgc atcaggctca gccgtatcgt cgatgcgctc gactcgaacc
     3301 tccagggcaa cgtccacggt ggcgcgatca tgcggctcgt cgacgaggcg gccggcgtgg
     3361 tggcctcccg gcacagcggc ggccgtgcgg tgacggcagt cgtcgacgag ttgcagttcc
     3421 tcgtgcccgt gcatgttggc gacatcgtga cctgcgacgc ccaggtcaac tggaccgggc
     3481 ggacgtcgtg cgaggtcggc gtccgggtga ctgcgcagcc gtggaaccag gccggcgatg
     3541 tcggccgcca cgtcgcgtcg gcgtacctag tcttcgtcgc catcgatgac gacgagcacc
     3601 cgcgcacgat cccgccggtg atgccacaaa acgatgaaga ccgcaggagg tttcgcgagg
     3661 ccgagatccg tcgtggtacc cggttggccc ggcgcgaggc aatcctgcgt tcacgtgaac
     3721 gcgggagcgc tcagccctga acgcagctcg tgttccccgc gttcgtggcc ttgaggttcg
     3781 ggatcggagc cgccgtcgcg ggcgacgacg ccggtgcgct ggcgggtgca ctcgccgacg
     3841 gggaggccgt agcaaggcca ccccagtctt gaccgagcgt caagacgatg ttcgaacccg
     3901 agcttgagtc gagaacctcc tgcggcgtga cgctgagagc accgacgagt gtctgcatcg
     3961 acgtggtgtg acttgcctga taggccacgg tggtctttgc gacgggactg ttcgatgcgg
     4021 tgccgatcga cacgacgtcg aaccctgcgg cactgagttc gtcgcgcacc tggcgggcga
     4081 ggccgtcggt cgacgtcccg ttcagcacct cgaccgacac tgccgagcgc ggcagcgtga
     4141 tgttcgccac gttcgacccg ctcgtgaccg ggatcgcgcg gtcgtcgtga atgtcggcga
     4201 acaacgcggt ggcttcgtcc tcgctccagt agaggtgctc gtagaacggg tcacccttgg
     4261 gcgccggata ggtcggcacg gtgacgaaca cgatgttcga tgtgttcgtg tgccggtagt
     4321 tgatgccgag cgagagcagt tgcgagctcg tcatgtcggt gctcagtgag ctcgcgaccg
     4381 ctgagtagaa gctgtacatc gtcgctgggt tgaagaagtt cgcgctcgac agcgccttgc
     4441 gcaccatcga ggccatgaac tgttgctggc gctgaatgcg gtgcaagtcg tcgccgccgg
     4501 taaggccgta gcgcgccctg acaaacgcca gcgcctggtc cccctcgaga ttcaccggcc
     4561 ccgcgggcag gttcagcccg gtgttcgagt catggatggg actgcagatg ttgaggggca
     4621 cgccaccgat ggcctttgac atcttcacca cgccggtgaa gtcgatctcg aggaagttgt
     4681 cgatgcgcac gccgctcaga ctctccagcg ttttggccga acagtagggc ccgccgagct
     4741 cgatcgcctc gttgaacagc gccttcgtcg gcttgctgta ttggagctga ccgttcttgt
     4801 cgaggatcgg attgccgtcg ctgtcgacct tttcgcatgc ggggatctgg acgacggagt
     4861 cgcgcgggat gctgacgagc gtcacgtctt tgccgttggc tgccaggtgg gcgaggatcg
     4921 ccgtgtcaga aagcgattcg gtctgcgccg gcgcaccctt gacctggaag gtgctgttct
     4981 caccgaggcg actgtcgact ccgaggatca gaatgttctt cgcggttccg ccgccagcac
     5041 cgccagcagc gccaccgccg gtcgcagtat taggcttcgc cgccggttgg ggtcgtgcgc
     5101 tggccaccag cacatgactc agcgactttt ggaagtggac cttgccgaag atctccttga
     5161 aggcaacgcc gacgatcccg ccgatgagca cgaccacggc gaagaagccg acgatgaggc
     5221 ggccgatgat gcgcgcccga cgctgcgcgt gctcatacgc cgcctgctca cccgcaggat
     5281 cgagcacctt tgggcggcct gtcgggggag ccataggccg gattctcctg gtaggtcggt
     5341 cgactcatct cgcggctcgg catctcttgg catctgacgc ccccgggtcc gaccgcgccc
     5401 tatcgtaacg gtcgggctgg cccgccgcgt tcccggtcag tcggtgcacg aggcgttcgc
     5461 cgccgtcgtc ccgcccgtcg tctcggcatt tgcagggctt gtcgctgtgg tgctggggct
     5521 ggcactggcg ccaggtgtgt gtgcgccggc gtaggaggat ccgaccgtta gttcgagggt
     5581 cgtgtcgagt tgcgggttgg cctgcagcgt cgaaccgggg atcgcggcct gcaacgtgcg
     5641 cgccgcctca gcctgcgtag gcccatacgc gatgacgctg cccgatgcgt ggttgatgcg
     5701 atcggacggt gtgccaacaa gctgaaaacc aagcttcgac agatcggacg ccgctcggcg
     5761 ggcgaggccg gtaacgccgc cgccgttgaa cacctggaca tgcaccgcac ttgccggcac
     5821 ggtcagcgcc gcggccggtg ctgctggtgc tgccgcgctc gtcgtcgccg ccaccggcgc
     5881 gtcgatcgcc agggacgaga acagtgcggt ggccttcggt acgtcgacgt cgtcgacatc
     5941 gacgccgttt tgggtgctga agccagagag cggcatcgtc gtcagcacga tgttgccggt
     6001 gccgagaccc ttgagcttca aggccagctt ggcgagatca gccgcactca tacccgaatc
     6061 gacgctgatc gacttcgtca gcgcgtcaag gaaatcgttc agcttcaacg ggttcgtcag
     6121 cgtgccagcg ctttcgacct tgcgcaccat cgatgccagg aattgctgct ggcgcttgat
     6181 ccggtcgatg tcgccgagcg gcagtccgta acgctgccgc acgaacgcaa gtgcggtctt
     6241 cccgtcaagg tggtgcacgc ccgccgacag attcagtcca gtctgcacgt cgtgcgcgga
     6301 tttcgagagg cacacgtcga cgccgccaag tgagtcgacc atgctctgga aacccgcgaa
     6361 gtcgatctcg acgtagtggt cgatgtgaat ctcggtgagt tgctgcaccg tgctcacgag
     6421 cagggccgca ccgccaagag agaaggcggc gttcaacttg tccatgtgcg cggggtgcac
     6481 gacacccttg gcgtcggtgt acgccgggat cgtgacccac gagtcgcgcg gtaacgagac
     6541 caaggttgcc gcctgatggc ccttcgccag gtgcgcgagg atcacggtgt cggatcgggc
     6601 gccctcgacc gccgtctggc ccttgggtgc ctgcgcgaat tggtcccctg ccccggttcg
     6661 gctgtcggag ccgaccagga gcacgttctc agcgccgtcg gtcgccgccg cgccacccgg
     6721 cttgatgacg ccggcggccc ttgtgaggtt gtggtcgtag tgcaggtagc gggtgtagag
     6781 caacccgctc gccgcgagca cgcagaccgc aagcaccgct gccgtgatcc cgagcgggct
     6841 gcgccaagta ccgcgtttgg tcggtcgtga gggtttgcgc ggtttgcccg atttagcggg
     6901 acgtcgagtg ccgcgcgggt tgagttctgg cggcagcgcg aggtgttccc cagggaagcc
     6961 ggggtgaccg gggcgctcgg gcggcaggcc gggatggccc atgggagcgc tttgatctgg
     7021 cacggtctgt cgtgtctcca gttagagggg tgttcacgcg tcttcgaggc taaccggcgg
     7081 ctggcgccaa gccctgcaat cactgacgcg acgcgcgcac ccttcgttgt atcccgactc
     7141 gttgtatccc gacgtccggt accacgcgct cccgcggtgc aaccaccgct gcccgaacaa
     7201 gttgtggacg tttaccgccc cctgagggca gtaaacgtcc acgctgtggg ccgatccgag
     7261 acgctttcgt gacccatgcc ccggaacgcc gcactcgaag gccaacgtca gaggtgcgac
     7321 caccgacctg gaggcgacga tgcaggtttc cgactcgacc acgcgggctc gacctcgccg
     7381 ggcacaagag gtaggcggcg tatgccttgc tggggtgcta ttggtcgggt tgacggcctg
     7441 cggcagtcag cgcgccagca ccagctcagg cgtaacgggc cccgtctcga gcggcagcgc
     7501 gcttgtctcg agcggcagcg cgcttgtctc aaacggcagc gcgcccgtgt cgagcagcag
     7561 tgccccgatc ctgagcagca atgccccgat ctcgtgcagc agcgtgccgg tgcccttcga
     7621 cagcccaacg aggtacgtgg gtgccgcggg ggtgccggac atgacggctg ttctggattg
     7681 gatacggtcg ggctcgaccg cgagagggac gatggggctc tcgggacacc ctgagccgat
     7741 aaccgagatc ccgcctggca gctacccact gtgcatctcg ttcgtgggcg actcgttcac
     7801 cgggcggctt gaggtcaagg gtcaaccagt gcccatctcg ggacagctca cggcgacggg
     7861 cgtcgatgtg gagttcgatg cctcacccgc catggatcca accctgcgtt tcacgagcat
     7921 cggccccggt agttacagct atccgccaag ccatccggca agttgacgat cgatccgcag
     7981 ggaagtctca ggaagctagc tccggtgccc gaaacgatgc tccggtagcc tctccgggca
     8041 tgaaggcact cgtattgtcg ggcggcgccg gcacccgctt gcggcccatc acccacacct
     8101 cggccaagca gctggtcccc gtcgccaaca agccggtgct cttctacggc cttgaggcca
     8161 tccgcgacgc gggcatcacc gatgtcggca tcatcgtcgg tgacacgcac gcggaaatcg
     8221 aagcggcggt cggtgacggc tcggcgctcg gcatcaaggc cacctacatc ccgcaggacg
     8281 cgccactcgg cctcgcgcac tgcgtgctca tcgctcgtga tttcctcggc aacgacgact
     8341 tcgtcatgta tctcggcgac aacttcatca tcggtggcat cactgatctg gtcgatgagt
     8401 tcaagcgtgg cggtgccgac gcgcagatcc tgctgacccg cgtcgacaac ccgcagcagt
     8461 tcggcatcgc cgaactcgac gacgccgggc acattgtgcg tcttgtcgag aagccggccg
     8521 aaccgaagag cgacctggcg ctcgtcggcg tctacatgtt ctcgccgacg atccacgagg
     8581 ccgtcaacgc gatcacgccg agccgtcgcg gcgaactcga gattaccgac gcgatccagt
     8641 ggctggtcga ccacggcaag gacgttcgcc cgcacctggt caccgggtac tggaaggaca
     8701 ccggccgtct cgaagacatg ctcgagtgca atcgcaaggt gctcgagtcg gtcgagccga
     8761 gttgccttgg cagcgttgac gacctcagtc gcatcatcgg cagggtcgtc atcgaggagg
     8821 gcgcctcgat cgtccgctcg accgtgcgcg gcccagcgat cattggccgg gacacccgca
     8881 tcgtcgacag ctacgtcggg ccgttcactt cgatttacca cgactgcgtg atcgattcga
     8941 ccgaggtcga gcactcgatc gttctcgaat cgacgaccat taccggtgtc agccggatcg
     9001 aggattctct tattggcaaa gaggtcgagg tctcgccgtc gaccgccatg ccgcgggctc
     9061 accgcctgat gctgggcgac cacagcaagg tgtcactcgc ctgagccccc gcatgcgtgc
     9121 ctccgccgat ccgacctccg gtcgacgtat cgatgccgac atcgtcggcg tcgcgactct
     9181 cacgatctcg gcagcagcgg tgtacaccgt gttcgagatc gagcagcagc gccacttcaa
     9241 caccgcaggc tacgacctgg gcatcttcga ccaggcgatc cacagttacg cccacttcca
     9301 tgcgccgatc tcgttgatga agggcgtgca caacggcttc ggcacgcatt tttccgaact
     9361 gggcgaccat ttctcaccga tcatcgcggc ggtcgcgccc gtctaccggg ttttccccga
     9421 cgcggtgacg ctgctgatcg tgcagggcct gctgctcgcg gcgtccgtgc cctcgggttg
     9481 gct
//
LOCUS       DASWVF010000680         4390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401525, whole genome shotgun sequence.
ACCESSION   DASWVF010000680 DASWVF010000000
VERSION     DASWVF010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401525"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGD55_11990"
     CDS             <1..675
                     /locus_tag="VGD55_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0871113.1"
                     /translation="EQVGLKVVASADDQPAAVVQGYSPDLAYADLAETALAVRSGALW
                     VATNVDSTLPSRRGLLPGNGSLVAAVALATGQRPVVAGKPELPLHAEGVTRTHAKNPL
                     VVGDRLDTDIEGAFAADTDCLLVLTGVTRAADLVDAPIKHRPTYLALDLSGLIEPQPD
                     VEWDGTVARCGRWVATATSSEIRVDGEGQALDGLRSLLTAAWAARPLEGVRVDASAAL
                     RQLGFG"
     gene            complement(682..1593)
                     /locus_tag="VGD55_11995"
     CDS             complement(682..1593)
                     /locus_tag="VGD55_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY0871114.1"
                     /translation="MTNLRIESVHSHAFWDRSLEPVLRVAPGAEISLELRDASGGQIT
                     PDDGPDAIARLDLGRVNPCTGPIAVDGLEPGDGLVVTILDIEPAPWAWTANIPGFGLL
                     ADDFPDAHLWISHVEVGRIHTPIGVELETRPMVGTIGVAPVTYGRHPILVPAEHGGNM
                     DICQLGIGTELHLPVLVPHALFSAGDSHALQGDGEICGTGAEMGSTLTVRLDRAPAPH
                     RGTPWFEHEAPALGTRFAATTGIGPDLFEAARDASRRGVELVVTRTGLAPIDAYLLLS
                     LIGDLRISEIVDAPNWVVSLHIPIRYL"
     gene            complement(1601..1933)
                     /locus_tag="VGD55_12000"
     CDS             complement(1601..1933)
                     /locus_tag="VGD55_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY0871115.1"
                     /translation="MATPEECRVAIGQLAARLAGPGDKRAEGLDRSVSAAVTDLDIVF
                     RGHLHDGVLDDVTTDEGPAAQIRLTLTSDDLVLIAGGELSLGSAFMSGRMKLHASLPD
                     MLRLRTLI"
     gene            2064..2300
                     /locus_tag="VGD55_12005"
     CDS             2064..2300
                     /locus_tag="VGD55_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871116.1"
                     /translation="MSDDLYDADQDEATAEAADQAVDERPQPSVPVPTGVPSVDVAVE
                     RLAELDALPTSEHVAVYDAVHRQLQDALADLDGA"
     gene            2325..3128
                     /locus_tag="VGD55_12010"
     CDS             2325..3128
                     /locus_tag="VGD55_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0871117.1"
                     /translation="MRRARLDAELVRRGLARSRNQAAELVTAGRVTVSGQVATKAATA
                     IGPDDPLVVISDADEPAYVSRGGHKLAGALEVFGPLGLAISGRRCLDAGASTGGFTDV
                     LLRAGAASVVAVDVGYGQLAWSLQTDARVVVVDRTNVRDLTLDVIGTPVDVVVGDLSF
                     ISLRIVLPALVGVLASEGDLVLMVKPQFEVGRERVGTGGVVRDASARADAVRGVADAA
                     ATLGFGVRGIVASPLPGPAGNVEYFLWLHASAAPLDDLELARAIEEGPH"
     gene            3151..4047
                     /locus_tag="VGD55_12015"
     CDS             3151..4047
                     /locus_tag="VGD55_12015"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY0871118.1"
                     /translation="MSRSVLLVCHTGRAEAVTAAREVARFLADAAIDVRVLETEADEL
                     DIPFAVGVPSTPDGARGAEAVIVLGGDGTLLRAAEVAREAEVPLLGINLGHIGFLAEA
                     EPADLAGTVRRIADRDYKVEERLTLDVEVTVDGEVVWRNWALNEASVEKSNRERMMDV
                     VVEVDGRPMSQFGCDGVVMATATGSTAYAFSAGGPILWPDVEALLVVPLNAHALFARP
                     VVVTPKSTLAVDILGNEPIGVVWCDGRRGHALEPGARVQTRGSDRRLKLARLRHGPFT
                     DRLVAKFGLPVRGWRGAAGLPE"
     gene            4057..>4390
                     /locus_tag="VGD55_12020"
     CDS             4057..>4390
                     /locus_tag="VGD55_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0871119.1"
                     /translation="MLEELRISGLGVIDDAVLDLSPGLTVVTGETGAGKTMVVTGLGL
                     LFGGRADAGLVRAGSTRAAIDGRLRITPGGQVAGRVDELGGDLDDETLLLSRIVGADG
                     RSRAQVGGR"
BASE COUNT          676 a         1366 c         1592 g          756 t
ORIGIN      
        1 gagcaagtcg ggttgaaggt cgtcgcgtcg gccgacgacc agccggccgc ggtggtgcag
       61 ggctactcac cagaccttgc gtatgccgac ctcgccgaaa cggcgctggc ggtgcgatcc
      121 ggtgccttgt gggttgcgac gaatgtcgac agcacactgc cgagccggcg tggattgctg
      181 cccggcaacg gatcgctggt cgccgccgtt gctctggcga ccgggcagcg tcctgtcgtc
      241 gcaggtaagc cggagttgcc actgcatgcc gaaggcgtga cgcgaacgca cgccaagaat
      301 ccgctggtgg tcggcgatcg cctcgacacc gacatcgagg gggcgttcgc ggccgacacc
      361 gactgcctgc tcgtgctgac cggtgtcacg cgtgccgctg acctcgtcga tgcaccgatc
      421 aagcaccggc cgacctatct ggcgctcgac ctttccggct tgatcgagcc ccagcctgac
      481 gtggagtggg acggcaccgt cgcgcgctgc ggtcggtggg tcgccactgc gacaagcagt
      541 gagatcaggg tggacggcga ggggcaggcg ctcgatggcc tgcgctcctt gttgacggct
      601 gcctgggcgg cgcggccgct cgagggtgtg cgagtcgatg cgtcggcggc cttgcgccag
      661 ctggggtttg ggtgaatcag ctcagagata ccggattggg atgtgcaggc tgacgaccca
      721 gttcggcgca tcgacgatct cggaaatgcg caggtcgcct atgaggctca gcaggaggta
      781 tgcgtcgatc ggagcgagcc cggtgcgggt gacgaccaac tcgacgcccc tgcgcgaagc
      841 atcgcgcgcc gcctcgaaga ggtcgggtcc gatgcccgtg gtcgccgcga accgggtgcc
      901 gagcgcggga gcttcgtgct cgaaccaagg cgtgccgcga tgcggcgcgg gtgcacgatc
      961 caggcgcacc gtcagggtcg agcccatttc ggcaccggtg ccgcagatct cgccgtcgcc
     1021 ctggagcgca tgggagtcgc ccgcggaaaa caacgcgtga gggacgagca ccgggaggtg
     1081 gagctcggta ccgattccca gctggcagat gtccatgttc ccgccgtgtt cggcgggcac
     1141 gagtatgggg tgccgcccat acgtgaccgg agccaccccg atggtcccca ccatcgggcg
     1201 tgtttctaac tcgactccga tcggcgtgtg tatccggccg acctcgacgt gagagatcca
     1261 caggtgagca tcggggaaat cgtcggcgag caggccgaac ccggggatgt tcgcggtcca
     1321 ggcccatggg gctggctcga tgtcgaggat cgtgaccacg agaccatcgc ctggctcaag
     1381 gccgtcaacg gctatcgggc ctgtgcaagg gtttacccgg cccaaatcca ggcgagcgat
     1441 ggcatcgggc ccgtcatcgg gagtgatctg cccgccgctc gcatcacgaa gttcaagtga
     1501 gatctcggcg cccggagcaa ctcgcagaac gggctcgagc gaacggtccc agaacgcgtg
     1561 gctatggacc gactcgatgc gcaggttagt catggcgtgc ctagatcagc gtccgcagcc
     1621 tcaacatgtc gggcagcgac gcgtgcagtt tcatccggcc cgacatgaat gcggatccga
     1681 gcgagagttc gccgccggcg atgagaacga ggtcgtcgct ggtcagcgtc agccggatct
     1741 gagcagccgg cccctcgtcg gtcgtgacgt cgtcgagcac tccgtcgtgc aggtgccccc
     1801 gaaacacgat gtcgaggtcg gtcaccgcgg cgctcaccga ccgatcgagt ccttcggcac
     1861 gcttgtcccc gggcccggcg agccgtgccg ccaattggcc gatggccacc ctgcactctt
     1921 cgggagttgc cacgatgttc ctctcgctct gcccccggct cgtcggctgg cgggacgtcg
     1981 ggaggctacc tcagcgccga gcgcaacggc tggggccacg ccgtcgccga gcatcggccg
     2041 atctggaccg ataccgtggc ggtgtgagcg atgacctgta tgacgcggac caagacgagg
     2101 cgaccgccga ggccgcggac caggctgtgg acgaacgccc gcagccgagc gtgcccgtgc
     2161 ccaccggcgt cccgagtgtt gatgttgccg ttgaacggct cgccgaactc gatgcgctgc
     2221 ccaccagcga gcacgtcgcc gtctacgacg cggtgcaccg gcaactccag gatgcgcttg
     2281 ccgatctcga cggcgcctga gagccctgag agcccattgc cgtggtgcgc cgcgcacggt
     2341 tggacgccga actggtacgc cgcggccttg ctcgctcgcg caaccaagcc gctgagcttg
     2401 tcacggcggg gcgggtcacg gtgagcgggc aggtcgccac gaaggccgca acagcgatcg
     2461 ggcctgacga tccgttggtc gtgatatcgg acgccgacga gccggcgtat gtgtcgcgtg
     2521 gaggccataa attggccggc gccctcgagg ttttcggtcc tcttgggctc gcgatttcgg
     2581 gccggcggtg ccttgacgca ggtgcgtcga ccgggggatt caccgacgtg ttgttgcgtg
     2641 ccggcgccgc gagtgtcgtt gcggtcgacg taggttatgg ccaactcgcc tggtcattgc
     2701 aaacagatgc gcgcgtggtt gtggtcgacc gcaccaacgt gcgcgatcta acacttgacg
     2761 tgatcggaac acccgtcgac gtcgtcgttg gtgatctgtc gttcatctcg cttcgcatcg
     2821 tgttgcccgc gctggtaggt gtgctcgcca gcgagggcga tctggtgctg atggtcaaac
     2881 cgcaattcga agtgggtcgc gaaagggtcg gaacgggcgg agttgtccgc gatgcgtcgg
     2941 cgcgcgctga cgccgtgcgg ggggtcgctg atgcagcggc gacgcttggg ttcggtgtgc
     3001 ggggtatcgt cgcgagcccg ctacccgggc cggcggggaa tgtggagtat ttcctgtggc
     3061 tgcatgcaag tgcagctccg ctcgacgatc tggagttggc tcgggcgata gaagaaggac
     3121 cgcattagtg actgcagtgg aaggaaaact gtgagcagaa gcgtcttgct cgtctgtcac
     3181 accgggcgcg ccgaggcggt gacggcagcg cgggaggttg cacgcttcct tgccgacgcc
     3241 gcgatcgacg tgcgcgtgct cgagaccgag gcagacgagc tcgacattcc ctttgctgta
     3301 ggcgttccca gcacgccgga cggcgcacgc ggagccgagg ccgtcatcgt gcttggcggt
     3361 gacggcacgc ttcttcgggc cgccgaggtc gcgcgcgaag ccgaggttcc gctgctcggc
     3421 atcaaccttg gccacatcgg attccttgca gaagctgagc cggccgacct cgctggcacg
     3481 gtccggcgga tcgccgatcg cgactacaag gtcgaggagc gcctgacgct cgacgtcgag
     3541 gtcaccgttg atggcgaggt ggtgtggcgg aactgggcct tgaacgaagc gtcggtggag
     3601 aagtcgaatc gcgaacgcat gatggacgtc gttgtcgagg tcgacggccg cccgatgtcg
     3661 cagttcggtt gcgacggcgt ggtgatggcg acggcgacgg gttcgacggc gtacgcgttt
     3721 tccgccggcg ggccgatcct gtggccagat gtggaggcac tcctcgtcgt cccgctcaat
     3781 gctcatgcac tttttgcccg gcccgtcgtg gtgacgccga aatcgacgct tgcggtcgac
     3841 atcctcggga acgagccgat cggggtggtc tggtgtgacg gacggcgcgg ccatgccctc
     3901 gaaccgggcg cgcgggtgca gacccggggc agcgatcgcc ggttgaagct cgccagactc
     3961 cgtcacgggc cgttcaccga ccgattggtg gccaagttcg ggctgcctgt gcgcggttgg
     4021 cgcggcgcgg ccgggttacc cgaatagcat cgacgggtgc tcgaagaact gcggatcagc
     4081 gggttgggcg tcatcgatga cgccgtgctc gatctttcgc ctgggctaac ggtggtcacc
     4141 ggcgagaccg gtgccggcaa gacgatggtc gtgaccggcc tcggcctttt gttcggcggt
     4201 cgcgccgacg cgggcctcgt gcgcgcgggc tcgacgcggg ccgcgatcga cgggcggctg
     4261 cgcatcacgc ccggaggcca ggttgccggc cgggtcgacg aactcggcgg tgacctcgat
     4321 gacgagacat tgctcttgag caggatcgtc ggggccgatg ggcggtcgcg ggcgcaggtg
     4381 ggtggccgtt
//
LOCUS       DASWVF010000681         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401537, whole genome shotgun sequence.
ACCESSION   DASWVF010000681 DASWVF010000000
VERSION     DASWVF010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401537"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGD55_12025"
     CDS             complement(<1..273)
                     /locus_tag="VGD55_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0871120.1"
                     /translation="MSARSTSRASLSIGEVLTHLRTDFPDVTISKIRFLEAEGLVEPD
                     RTSAGYRKFSHADVQRLRYVLTAQRDHYLPLRVIKDHLDALDRGLAP"
     gene            complement(285..770)
                     /locus_tag="VGD55_12030"
     CDS             complement(285..770)
                     /locus_tag="VGD55_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0871121.1"
                     /translation="MSSERQDSTTTISISGLEVLEPSPDDEGLADQAAIDGLPPGAGL
                     LVVRRGPNAGSRFLLDADVTTAGRHPESDIFLDDVTVSRRHAEFRKTADGFFVRDVGS
                     LNGSYVNRERIDDASLQGGDEVQIGKFRLVFLTGRAAPSPGSTAGDTGDTGTTGHTRA
                     Q"
     gene            complement(1025..1429)
                     /gene="gcvH"
                     /locus_tag="VGD55_12035"
     CDS             complement(1025..1429)
                     /gene="gcvH"
                     /locus_tag="VGD55_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0871122.1"
                     /translation="MLALEVHVIPDDLHYTAEHEWVKVIDGGIRVGITDHAQEALGDI
                     VFVTLPAPGTQVEAGQPLGEVESTKSVSDIYAPVSGAVLARNEALDARPELVNADTYG
                     EGWMIEIAPTDPAAVHALLDAAAYTSLVSSSE"
     gene            complement(1444..2346)
                     /locus_tag="VGD55_12040"
     CDS             complement(1444..2346)
                     /locus_tag="VGD55_12040"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY0871123.1"
                     /translation="MTDADAADEPAEPTEPAEPDGTPSARTPLQMLVDAGRPRASRGQ
                     LIGALLCGVLGFALVTQVHSAAGLGFTTARQSDLVDILDSLSARSDQLRAQIATEQTS
                     LSKLTGGTDQTQAALDEAQSRAQTLQILAGTVAAMGPGIELQISDPQHQVGADVLLEA
                     LEELRDAGAEAIQITGSAAATGATPTDRAGVTATTSPTDRAGASGRAVRLVASSYFLD
                     ATDTSGVIVDGVLLAPPYDIIAIGDPQTITTAMGIPGGVLETLTGKGATGTVIQHDVV
                     RVTALHVLATPQYARPAPALSSAG"
     gene            complement(2343..>2453)
                     /locus_tag="VGD55_12045"
     CDS             complement(2343..>2453)
                     /locus_tag="VGD55_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HEY0871124.1"
                     /translation="GDQLGVGSQLSTGVVVVLGIRIFSNAAAIRRHLFRA"
BASE COUNT          399 a          790 c          830 g          434 t
ORIGIN      
        1 cggtgcgagg ccgcggtcga gtgcgtcgag gtgatccttg ataacccgca gtggcagata
       61 gtggtcgcgc tgtgcggtga gtacgtagcg cagccgctgg acgtcggcat gggagaactt
      121 gcggtagccg gccgaggttc gatcgggttc gaccaatcct tcggcctcga ggaacctgat
      181 cttcgagatc gtgacgtcgg ggaaatcagt gcgcagatgc gtcagcacct caccgatgct
      241 caaggacgca cgcgatgtcg accgggcgct cacggctgcc atcatcactg cgctctggta
      301 tggccagtgg tgccagtgtc gccagtgtcg cctgctgtgg agccggggct cggtgcggcc
      361 cggccggtca ggaagaccag cctgaacttg ccgatctgca cctcgtcgcc gccctgcaac
      421 gaagcgtcgt cgatccgctc ccgattcacg tagctgccgt tcaagctgcc aacgtcgcgc
      481 acgaagaagc cgtcagccgt cttgcggaac tcggcgtgcc ggcgtgacac ggtgacgtcg
      541 tcgagaaaga tgtcgctctc tggatgccgg ccggccgtgg tcacatcggc atcgagcagg
      601 aatcggctgc cggcgttcgg gccgcggcgc acgacgagca ggcctgcccc aggcgggaga
      661 ccgtcgatgg ctgcctgatc ggccagcccc tcgtcgtccg gcgaaggctc taagacttca
      721 agaccggaga tcgagatcgt cgtcgtggag tcttgacgct cggagctcac ggggtgaccc
      781 ggcctgttca gggcgtgacc gcagctcgcg cagaaccgag agtcgtcagg attctcgtgc
      841 ccgcaattcg tgcagaagat cgtcggcatc gggttcgtcc ctccccctca ggttcgattg
      901 gtctttcggg ctgcgaccgt caggtagagg ttgacggtca gggcaaccta tgcgcgcaac
      961 ggacgccggt caactcgtca cgctactcag gaagcctggc aggttactga gaagcctgtc
     1021 gggcttattc agaactgctg acgagcgacg tataggccgc cgcgtcgagc agtgcgtgga
     1081 cggcggctgg gtcggtcggt gcgatctcga tcatccagcc ttcgccgtag gtgtcggcgt
     1141 tgaccagctc tgggcgcgcg tcaagcgcct cgttgcgggc aagcaccgca cctgagacag
     1201 gcgcgtagat gtccgacacg ctcttggtcg attcgacctc gccgagcggc tgaccggcct
     1261 cgacctgcgt gcccggcgcg ggaagggtga cgaacacgat gtcgccgagg gcctcttgcg
     1321 cgtggtcagt gatgccgacc cgaatgcctc cgtcgatgac cttcacccac tcgtgctcgg
     1381 cggtgtagtg caggtcgtcg ggtatcacgt gaacctccag cgcgagcatc ggacgagcat
     1441 cggtcaaccg gcagatgaga gagcaggcgc cgggcgagcg tactgaggcg tggcgagcac
     1501 gtgcaacgca gtgacccgga cgacgtcgtg ctggatcacc gtgccagtag cgcccttccc
     1561 tgtcagtgtc tcaagcactc ccccggggat ccccatcgcc gtcgtgatcg tctgcgggtc
     1621 gccgatggcg atgatgtcgt aagggggggc gagcaggacc ccatcgacga tcaccccgct
     1681 ggtgtcggtt gcgtcgagga agtagctgct ggcgaccaag cggaccgcac gccctgaagc
     1741 tccggcccga tccgtcgggc ttgtggtcgc tgtgacacca gctcgatcgg tcggcgtcgc
     1801 accggtggcc gccgcgctgc cggtgatctg gatcgcctcg gcgcccgcgt cgcgcaactc
     1861 ctcgagtgcc tcgagcagta cgtcggcacc gacttgatgt tgcggatcgc tgatctgcaa
     1921 ttcgatgccc gggcccatcg ccgccaccgt gccggccagg atctgcagcg tctgcgctcg
     1981 cgattgcgct tcatccaacg ccgcctgcgt ctgatcagtg ccacctgtca gtttcgacag
     2041 cgacgtctgc tcggtcgcga tctgggcacg aagctgatcg gagcgcgccg acaggctgtc
     2101 gaggatgtcg acaagatctg attgccgcgc tgtggtaaac ccgagcccgg ccgcgctgtg
     2161 cacctgggtc acgagggcaa accccagcac cccgcacaac agggcgccga tcagttggcc
     2221 acgactcgcc cgtgggcggc ccgcatcgac gagcatctgc aagggagttc gggcactcgg
     2281 cgtcccgtcc ggttccgctg gctcggtggg ctcagccggt tcgtccgccg cgtcggcgtc
     2341 ggtcatgcgc ggaatagatg tcgccggatc gctgcggcgt tggagaagat ccggataccg
     2401 aggaccacga cgacgccagt cgacaactgg ctacccacac cgagctggtc gcc
//
LOCUS       DASWVF010000682         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401561, whole genome shotgun sequence.
ACCESSION   DASWVF010000682 DASWVF010000000
VERSION     DASWVF010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401561"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VGD55_12050"
     CDS             <1..1101
                     /locus_tag="VGD55_12050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0871125.1"
                     /translation="RTRQDRVSRELLTVWLLPIALVLPPAYVMISLIAARVYLYLRVW
                     HAQAIKAVYNTAAAGLAYGTASLLHRALLHASSKTHLTPAQFTGGAAAAAILLISVAA
                     YCVVHSVLVGAVVVLTAGKEALGNFLKDREGLVVDIVGICLGIVAAVAWVADPVFAVF
                     LLPPVLLLEHQLFSGLRRAVRTDLLTEVANPQYWREVAGREVERASSSGDHLAVLMVD
                     IDHFKAVNDKYGHLAGDEVLAAVARTVAQALRPRDLVGRLGGEEFGAVLSGLNLLDAE
                     GAAERLRSQVRDVRVRADSGDWISVTVSVGVSELSVTGGDLRQLLDAADVALYAAKAA
                     GRDCVRAATREPGTVIDLSIPDQRAPAAEKSS"
     gene            complement(1117..2127)
                     /locus_tag="VGD55_12055"
     CDS             complement(1117..2127)
                     /locus_tag="VGD55_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0871126.1"
                     /translation="MPAVTVENPLTLPRVPLTDPALDRDRPVESVTTAPSGLEGEGFP
                     VRRAFHGVDLRALDPFIHMDQMGEVEYAPGEPKGTPWHPHRGFETVTYMIDGLFEHHD
                     SNGGGGLITNGDTQWMTAGAGILHIETPPEHLVASGGLFHGFQLWVNLPKAMKFAAPR
                     YQDIRASEVSLLASPDGGVLLRVIAGQVAGHAGPGITHTPIALVHATLSPGASLTLPW
                     NPDYNALVYTLAGRGSVGTGRRPISMGQLAVFGSGDTITIAADQTQETRSPTMDVLIL
                     GGKPIQEPVAMYGPFVMNTKAEVMQAFEDYQAGRLGTIPAEHAEHVDEAHAGVEGSTP
                     DA"
     gene            complement(2249..3280)
                     /locus_tag="VGD55_12060"
                     /pseudo
     CDS             complement(2249..3280)
                     /locus_tag="VGD55_12060"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
BASE COUNT          530 a         1097 c         1076 g          577 t
ORIGIN      
        1 cgcacccgcc aagaccgcgt ttctcgcgag ctgttgacgg tctggctgtt gccgatcgcg
       61 ctggtgctac cgccggccta cgtgatgatt tccctcatcg ctgcccgcgt ttatctgtac
      121 ctgcgggttt ggcacgctca ggcgatcaag gctgtttaca acaccgccgc ggccggcctg
      181 gcctatggca cggcctcgtt gttgcacagg gcattgctgc acgcgtcgtc gaagacccac
      241 ctgaccccgg cgcagttcac cggcggcgcg gcggcggcgg cgattcttct gatcagcgtc
      301 gcggcgtatt gcgtggtcca ctccgtgttg gtcggcgcag tcgtcgtgct gaccgcaggc
      361 aaagaagcgc tcggcaactt cctcaaggac cgcgagggtc ttgtcgtcga tattgtcgga
      421 atttgtcttg gcatcgtggc ggccgttgcg tgggtggccg acccggtctt cgcggtcttc
      481 ttgttgccgc ccgtgctctt gctcgaacac cagctcttca gcgggttgcg cagggccgtg
      541 cgcaccgacc tgctcaccga ggtggccaac ccgcagtact ggcgtgaggt tgccggccgc
      601 gaagtcgagc gagcttcgtc gagcggcgac cacctcgccg tgctgatggt cgacatcgat
      661 cacttcaaag ccgtgaacga caagtacggc cacctcgcgg gcgacgaggt cctggcagct
      721 gtcgcacgca cggtcgcaca ggcgttgcga ccacgcgacc tggtgggccg gctgggcggg
      781 gaggagttcg gcgcagtcct gagtggattg aacctgctcg atgccgaggg cgccgccgag
      841 cgcctgcgca gtcaggtgcg cgacgtgcgc gtgcgcgccg acagcggtga ctggatcagc
      901 gtgaccgtct cggttggtgt cagcgagttg tcggtcaccg gcggcgacct gcggcaactt
      961 ctcgatgccg ccgacgtggc gctctacgcg gcgaaggcag ccggtcgcga ctgcgtgcgc
     1021 gccgcgacgc gggaacccgg aaccgtgatc gacctgtcga tccccgacca acgggctccc
     1081 gccgcggaaa aaagcagcta agaaagacct gccaggtcaa gcgtccggtg tcgaaccctc
     1141 gacaccggcg tgcgcttcat cgacatgctc cgcatgctct gctggaatcg tgcccagccg
     1201 accagcctgg tagtcctcga acgcctgcat gacctcagcc ttggtgttca tcacgaacgg
     1261 gccgtacatc gccaccggct cctggatcgg cttgccacca aggatcagca catccatcgt
     1321 cgggctgcgc gtctcctgcg tctgatcggc cgcgatcgtg atggtgtcgc ctgacccgaa
     1381 gaccgcgagc tgtcccatcg agatcggacg ccgaccagta ccgacagatc cacgcccggc
     1441 cagcgtgtac acaagagcgt tgtagtcggg gttccatggc aacgtcagcg acgcacctgg
     1501 cgaaagcgtc gcgtgcacga gggcgatcgg ggtgtgcgtg atgccggggc ctgcgtgacc
     1561 cgcgacctga ccagcgatga cgcgcaacag cacgccccca tcaggtgacg cgagcagcga
     1621 gacctcactc gcgcgaatgt cctggtagcg cggcgccgcg aacttcatcg ccttcggcag
     1681 gttcacccac agctggaaac cgtggaacag gccgccgctc gctacgaggt gctccggcgg
     1741 ggtctcgatg tgcaagatgc cggcgcctgc ggtcatccac tgcgtgtcgc cattggtgat
     1801 gaggccaccg ccaccgttgg agtcgtggtg ctcgaacagg ccgtcgatca tgtacgtgac
     1861 cgtctcgaag ccgcgatgcg gatgccacgg tgtgcccttg ggctcccccg gcgcgtattc
     1921 gacctcgccc atctggtcca tatgaatgaa cgggtcaagg gcgcgcaggt cgacgccgtg
     1981 gaaggcgcgg cggaccggga agccttcacc ctcaaggccg gacggcgcag tggtgaccga
     2041 ctcgaccgga cggtcacggt cgagcgccgg atctgtcaac ggcacccgcg gcagcgtcag
     2101 cgggttttca acggtcacag caggcatcgg atcctcctcg atcggtggcc cttctgggct
     2161 accttcatcg tgccgaacag cggtctccgt gctgcaaaca ctgttgcaca cgcaatcatt
     2221 cccggcactc ctgcagcccg aaaagcgcct acagcccgag cagcgccggc aggtggcgcg
     2281 gcggcggatt gccgaacccg agcacggtgt cgtgaatcct gcggtccgac cacgccggct
     2341 gtgcgagtcg cagccgcccc acgacgtccg ccatctgctt gccaccaacg aaataggtcg
     2401 agagctgggc actgcttagc aacgcccgcc gccacttgcc ggtggcctca ccgtcttctt
     2461 gatgcccgcg tcctgtcatc agcgccatcg cgtcggcttc ggtgaggccg tcgcagtgca
     2521 cgcggacgtc gagtatcgcg ttgatcgtgg cgcgcatccg catcttgagt tgctgcaccg
     2581 ccacggtcgg gccgccgaat ccttgccgca ccatctcggc ctccgcgtac atcgcccagc
     2641 cctcgacgaa cggaccactc caataggcct ggtgcacctt cgtcggcacg gggccgcgcc
     2701 tgccgtgcgc gagttgcacg acgtggcccg gcatcgcctc gtgaatgacc agattgcgca
     2761 gcgcatgcac gttgcattcg cggaagaagg aggcgacccg ctcggccggt cagtcggcag
     2821 gcgtcggcga gatcgcaaag aacgtcgacg tgccgggcgg gtcgaggggc ccgggcgggt
     2881 cgcagtacgc gaccgctacc ccgcggtgga tctcgggcat cacgatgatc tcgaccggat
     2941 cgtcgtacaa cgtgatcagg tcgtgctcgg tgacgaagtc ggtcgtctcc gtcatcgcgt
     3001 ctttcgccag atccacgacc gtgtcgttcg tcggggcgtc gcctgccagc cgatcgagca
     3061 cctcacgggg agtgccgcca agagttgacg caatctcggc aagcttgtct tcgagcaacg
     3121 caagctcgtc gacggctgcc tgaagaacct gttcgggcgt catatcgaca tcgagcacga
     3181 ggctgagctt gcgagcgaag cgctcggccc ccaggcgcgg attgccgtcg gcgcggggaa
     3241 gttcagcgtt cagccactcg atgtgatgcg tgagcgccgt
//
LOCUS       DASWVF010000683         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401571, whole genome shotgun sequence.
ACCESSION   DASWVF010000683 DASWVF010000000
VERSION     DASWVF010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401571"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VGD55_12065"
     CDS             complement(<1..1380)
                     /locus_tag="VGD55_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0871127.1"
                     /translation="MACDANGETADLSKDVSPDTLAPNNRLVIYELPTAWSISRSTDE
                     PERATATFADVNAMVDATVGGANFAELPILAPGTSYLTALGVNALELLPCADSFYKRE
                     WGYDTSHYLAPDADLGYPEGNLSPTPNRDLATLIKSCHANGLRFFIDVVMAFGKEDPY
                     NHIDSQDFHLDDPAAAAKVNDPDAWTSFRSDGSRSFRNGFGSTLWRYANRVTTYDPVA
                     GTAGPIAPAAQFMLTYLTRWMEDFHVDGVRIDSVENVANWDFLSAFKNLARTMWKARW
                     TALGLPGDAESRFLVVGEELSLPFDLLRNGVLDGLWNDDFQRRARAAILGRNVDGEPT
                     FEWTVKKAIDCRLLTAAGFTDGTQAVIYLTKHDVEGAERLFTMLGRIFPGQPDQVERR
                     IKLGFVCLLTAVGVPMLLAGEEFADQHDLFDINGRVDQNGGKQIDPVNYSRITASGDD
                     DDGHNAPMRR"
BASE COUNT          283 a          547 c          538 g          302 t
ORIGIN      
        1 ccgccgcatg ggcgcattat ggccgtcgtc gtcgtcaccg gacgcggtga tccgactgta
       61 gttcaccggg tcgatctgtt tcccgccgtt ctggtcgacc cggccattga tgtcgaagag
      121 gtcatgctga tcggcgaact cttcccctgc cagaagcatg ggaaccccga ccgccgtcag
      181 caggcagacg aagcccagct ttatgcgccg ctcgacctga tccggctgac cggggaagat
      241 tcgcccgagc atggtgaaca gccgctcggc gccctcgacg tcgtgcttcg tcagatagat
      301 gaccgcctgc gtgccgtccg tgaagcccgc ggccgtgagc aggcggcaat cgatggcctt
      361 cttgacggtc cattcgaacg tgggctcgcc gtccacgttg cgccccaaga tggcggcgcg
      421 tgcgcgtctt tgaaagtcgt cattccatag gccgtccagc accccattcc tgaggaggtc
      481 gaagggcaac gagagctctt ccccgacgac caggaaccgc gactccgcgt cgcccggcag
      541 gcccagcgcg gtccaccgag ccttccacat tgtccgcgcg agattcttga acgcgctgag
      601 gaagtcccag tttgcgacgt tctccacgct gtcgattcgc accccgtcga cgtgaaagtc
      661 ctccatccag cgcgtgaggt aggtcagcat gaactgggcc gcgggcgcga tcggcccggc
      721 cgtgcccgcg acggggtcgt aggtcgtcac gcgattcgca tagcgccaca acgtgcttcc
      781 gaacccattg cggaatgacc gcgatccgtc actgcggaac gaagtccagg cgtccggatc
      841 gttgaccttg gccgcggcgg ccggatcgtc gagatggaag tcctgcgagt cgatgtggtt
      901 gtacggatcc tccttgccga acgccatgac cacgtcgatg aagaaccgca gaccgttcgc
      961 gtgacagctc ttgatcagcg tcgccaggtc gcgattcggg gtcggcgaca gattgccttc
     1021 ggggtagccg agatcggcgt ccggggccag gtaatgggac gtgtcgtagc cccattcgcg
     1081 cttgtagaag ctatccgcac agggaagcag ctccagcgcg ttgacgccca gggctgtcag
     1141 atacgacgtg ccgggcgcca ggatcggtag ctccgcgaag ttggctcccc cgaccgtcgc
     1201 gtccaccatg gcgttcacgt cggcgaacgt ggcggtcgcc cgttcgggtt cgtcggtcga
     1261 ccggctgatg gaccaggcgg tcggcagctc gtagatgacc agccggttat tgggcgcaag
     1321 cgtgtcgggg gacacgtcct tcgacagatc agccgtctca ccattcgcgt cgcacgccac
     1381 cagcgctcct gtcgaaagct tcacaacagc ggccggttgt gtggcgtcgc ccgcgcccgg
     1441 cgcgaaaacg cgccagtcca ccgacttcgc gaatgggtcc gtgacggcga tccgcgccgg
     1501 tgccggaagc gtcgagcgac tgtcatcgac ttcgaaccag tagtggtaga cggtgccatc
     1561 cgtcagtccc gcggccgtgc ccgcaatcaa ccagaccgaa gctctgcgtg ggtcacgcgt
     1621 cagcgacgtt ctggaaacgt tcttcagcgt cggggggttg cccgactgga
//
LOCUS       DASWVF010000684         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_19357, whole genome shotgun sequence.
ACCESSION   DASWVF010000684 DASWVF010000000
VERSION     DASWVF010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19357"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1341
                     /locus_tag="VGD55_12070"
     CDS             1..1341
                     /locus_tag="VGD55_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0871128.1"
                     /translation="MTASTEESCARTIATSLASRAYRRNVATSEIDELVTFYKTVRGL
                     SSTVTFASGVAAMIEVLLQAPEFLYRVEMGTPVAGSSDVKRVAGREMATRLSYLFWQT
                     MPDDTLFQAADAGMLDTNDGVSAQATKMVDDPRTKQMVAFFFDNLLPIPDLAGLTRSP
                     TLFPNWSSSIGVAMRSEVQRVLQYEIFENTSQSSPPYMPGSWPAILTAPYTFVNKALF
                     NYYGTSAFASGTPAVTTTDLTKVTLNPDQRVGLMTLGGIMAGLTTTDLTNPVLRGSFI
                     INKLMCKNLSVPTGLNPVAPDPYSAKTARERYGLHSASATCSVCHKSIDPLGLPFENY
                     DAIGLYRSAEHWVDPNTQMAYDTTIDAHGAVPGVDGAAKNGVELVKLLATSPEVESCF
                     ASHWMRFAYGRSLQSGDACNQQSVNTTFAGGGYNIKQLLLAVTQSDGFLYRPAQ"
BASE COUNT          267 a          502 c          495 g          247 t
ORIGIN      
        1 gtgacggcgt ccaccgaaga gtcctgcgcg cgcacgatcg cgacgtcgct cgcctcgcgc
       61 gcctaccgcc ggaacgtcgc gaccagcgaa atcgacgagc tggtgacctt ctacaagacc
      121 gtgcgtggcc tgtcgtcgac ggtcacgttc gcttcgggcg tggcggcgat gatcgaggtg
      181 ctgctgcagg ccccggagtt cctgtaccgc gtggaaatgg gaacgcccgt tgccgggagc
      241 agcgacgtca agcgggtggc cgggcgcgag atggcgacgc ggctgtcgta cctgttctgg
      301 cagaccatgc ccgacgacac cctgttccag gccgccgacg ccggcatgct ggacaccaac
      361 gacggggtgt cggcccaagc gacgaagatg gtcgacgatc cgcggaccaa gcagatggtg
      421 gcgttcttct tcgacaactt gctgccgatc ccggatctcg ccggcctgac gcgaagcccg
      481 acgctgttcc cgaactggtc gtccagcatc ggcgtggcga tgcggtccga ggttcagcgc
      541 gtccttcagt acgagatctt cgagaacacg tcgcagtcgt cgccgccgta catgccgggc
      601 agctggccgg ccatcctgac cgcgccgtac accttcgtga acaaggcgct gttcaactac
      661 tacgggacat ccgcgttcgc gtccggaacg ccggcggtca ccacgaccga cctcacgaag
      721 gtgacgctga atccggatca gcgggttggg ctgatgacgc tgggcgggat catggcgggc
      781 ctcacgacca ccgacctcac gaaccccgtg ttgcgcggca gcttcatcat caacaagctc
      841 atgtgcaaga acctgtcggt gccgacgggc ctgaacccgg tcgcgccgga tccctacagc
      901 gccaagacgg cgcgtgagcg atacgggctg cactcggcca gcgcgacctg ctcggtctgc
      961 cacaagtcga tcgatccgct gggcctgccg ttcgagaact atgacgccat cggcctttac
     1021 cggagtgccg agcactgggt cgaccccaac acgcagatgg cctacgacac gaccatcgac
     1081 gcgcacggcg cggtgccggg cgtggacgga gcggccaaga acggcgtcga gctggtgaag
     1141 ctgctcgcca cgtcgcccga ggtggaatcc tgcttcgcgt cgcactggat gcggttcgcg
     1201 tacggccggt cgcttcagtc gggcgacgcc tgcaaccagc aatcagtgaa cacgacgttc
     1261 gccggtgggg gttacaacat caaacagctc ctgctggcgg tcacccagag cgacggcttc
     1321 ctctaccgcc cggcccaata ggagacttgg tatgtcgcgt ttcaagctcg atcgtcgaac
     1381 ggtcatcaag ggcgccggca ccatagccat cgcattgccg tggctggaga tcatgggtcg
     1441 cgggtccaag gcacgcgcgc agaccgcgtc cgtgccgctg aagcggttcg tcacggtgta
     1501 ccagccgggc g
//
LOCUS       DASWVF010000685         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_503542, whole genome shotgun sequence.
ACCESSION   DASWVF010000685 DASWVF010000000
VERSION     DASWVF010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503542"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGD55_12075"
     CDS             <1..576
                     /locus_tag="VGD55_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0871129.1"
                     /translation="DPSGLPSYDVPTIGATNWAGGLSATEHLIELGHRRIGIITGPPR
                     LQCSRARLDGYRAAIEAAGLQVDQDLVHTGDFYHEAGFTGGSRFLDLADPPTAIFASS
                     DQMAFGVYEAARQHGLRVPSDLSVVGFDDLPEAAWTSPPLTTVRQPLAEMGMLAARTV
                     LRMVRGEAIETLRLELATQLVVRESTAAPRS"
     gene            676..>1721
                     /locus_tag="VGD55_12080"
     CDS             676..>1721
                     /locus_tag="VGD55_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0871130.1"
                     /translation="MPLARSRLVDAAVCAFTVGAALALVRLLSGAHSLTLRSDVLVVL
                     LASALGTTAVGFVRTRSDAALAAAIGKLDKVAAGDLMVSTAVVSSGHAGLVEAAIRDI
                     VVQQRQLLMETERSAELLGKGWKTINEVAWTMMDTSEGTVAEVTAAARAAGEVSENIQ
                     FIAGATQETTATMQEVASHAVDASGIGQSGVEQIAHAGKTVEDLQIASRRVDVVLALI
                     GQVARQTHLLALNATIEAARAGEHGRGFTVVAGEVKQLAEKTAQATGDVTATMREIEN
                     GSQRAVASINSVTETIQGMSSRQHSIAAAVEQQTATTHAIARSTAMAADQALALEGNV
                     KSLTNAVRLGAYAGA"
BASE COUNT          295 a          550 c          583 g          293 t
ORIGIN      
        1 gacccgtcgg ggttgccgtc gtacgacgtt ccgaccatcg gggcaacgaa ctgggccggc
       61 gggctcagtg cgactgaaca tctgatcgag cttgggcacc gccgcatcgg catcatcacc
      121 ggaccgccgc gacttcagtg cagtcgcgcg cgtcttgacg gttaccgggc cgcgatcgag
      181 gctgccggtc tgcaagtcga ccaggatctg gtgcacaccg gcgacttcta tcacgaggcg
      241 ggattcactg gcggttcgcg atttctcgat ctcgccgacc cgccgaccgc catcttcgcg
      301 tcgagcgacc agatggcgtt cggcgtctac gaggcggcgc ggcagcacgg gctacgcgtg
      361 ccgagcgacc tcagtgtcgt tggcttcgac gacttgccag aagcggcctg gacctcgccc
      421 ccactcacga ccgtgcggca gccactcgcc gagatgggga tgctcgcagc gcgcaccgtt
      481 cttcggatgg tccgcggaga ggcgatcgag acgctgcgcc tcgaactggc aacccaacta
      541 gtcgtccgcg aaagcaccgc cgcgcctcgc tcgtgacttg gctggcgggt gccctcaact
      601 cttgctcttc ggggccgatg agtcgatggg ccattctttc ggccaggagg cagtaggaag
      661 caccaggagg tatcgatgcc gctcgcgcgc tcgcgtctcg tcgacgccgc tgtgtgcgcg
      721 ttcactgtcg gtgcggcgct cgcccttgtc aggctgctga gcggcgccca ctccctgacg
      781 cttcggtctg acgttctcgt cgttcttcta gcctccgcgc tcggcacgac cgcggtcggc
      841 ttcgtgcgca cgcgctctga tgcagcgctc gcggccgcga tcggcaagct cgacaaggtc
      901 gccgctggcg acttgatggt ttcgacggcc gtcgtctcgt caggacacgc cggacttgtc
      961 gaagcagcga ttcgcgacat cgtcgtgcag cagcgccagt tgctgatgga gacggagcgg
     1021 agcgcggagc ttcttggcaa gggatggaag accatcaacg aggtggcctg gacgatgatg
     1081 gacacctcgg agggaacggt cgccgaggtg acggccgcag cgcgcgctgc cggagaggtg
     1141 tctgagaaca tccagttcat tgcgggcgcc acgcaggaga ccacggccac tatgcaggaa
     1201 gtcgcgtcgc atgctgtcga cgcgtcaggg atcggccagt ctggcgtcga gcaaatcgcg
     1261 cacgcgggca agaccgtgga ggatctgcaa attgcgtccc ggcgtgtcga cgtcgtcctc
     1321 gcacttatcg gacaagttgc tcgacagacc catttacttg cactgaacgc cacgatcgag
     1381 gcggcgcgag cgggcgagca cggccgcggc ttcaccgtgg tagccggcga agtgaaacag
     1441 ctggcggaga agaccgcgca ggcgacgggc gatgtcaccg cgacgatgcg tgagatcgag
     1501 aatgggtcgc agcgtgcggt cgcctccatc aacagcgtga ccgagacgat ccaaggcatg
     1561 agcagtcgcc agcattcgat cgctgcggca gtcgagcagc agaccgcaac gacgcatgcg
     1621 atcgcgcgca gcacggcaat ggcggccgac caagcgctcg ctcttgaggg gaacgttaag
     1681 tcgcttacga atgcggttcg actcggcgcc tatgccggtg c
//
LOCUS       DASWVF010000686         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_401636, whole genome shotgun sequence.
ACCESSION   DASWVF010000686 DASWVF010000000
VERSION     DASWVF010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401636"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VGD55_12085"
     CDS             <1..1102
                     /locus_tag="VGD55_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871131.1"
                     /translation="GGGAGTAGATAGTNRGGGGGAGTAGATAGTNGGGGKGGGAGSGA
                     GGASAGTVVAIPPYTISVWATGTNDYQAPDSIETDGTHIWVGYQNKTTKDGSDGGAGF
                     VANSTVVEYTLDGTTVLKTFSLAGHVDGLRVDPTNKNVWVTSNEDGAPHLFMIDATTQ
                     VVTSYVLPSPLPHGGGLDDIGFVDGKMFVAASAPSATKATQNRPALYQVTISGTTATL
                     TQALAGNGSATDYNVADAGVALNLTDPDSITISPTGDLVLVSQGDNQVLFVTHPGSTP
                     AIKSLTVATQLDDTVWTTNTKGQLLVVDGKANIIYSVRRSDGWTVGIVYTETPDDSTI
                     PNILGTLDLTTGNIQPIAVGFGKATGLLFLPQ"
     gene            complement(1234..1386)
                     /locus_tag="VGD55_12090"
     CDS             complement(1234..1386)
                     /locus_tag="VGD55_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871132.1"
                     /translation="MEIPWNFWCVSYWSNEMSNAIFGFLSNMTVLTHDDFPRRRTAAF
                     ETVSDP"
     gene            1495..2445
                     /locus_tag="VGD55_12095"
     CDS             1495..2445
                     /locus_tag="VGD55_12095"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEY0871133.1"
                     /translation="MTVNGQFYGLYLAEEGFTKSLLAQFLPGNTDGDLLKGGTEAHTN
                     IGTTNWTKVQALDSATDMGTLRTLVDVPNTVLESGAEAVVEDADGYYGGFHNFWIYDQ
                     GTAGYVWLLDHTDSALEWVEMFTPLGYKEHPIYWWMGRPLPDPPAKNYLLVINDPTAR
                     TQYVNAIATQVAKWNATEIVGWIDTWSKQIADAVGQDTHKWATTDQFWMAIAAMRDEA
                     QNRPQYLNGFLACENGDPSQASDQDGDGVAWCNDCDDSNASVHPGAPEVCGNHVDDNC
                     NGVVDENCPGEKPGYPGEPDGGVPPSPGADAGVLTDAGRG"
     gene            complement(2442..2768)
                     /locus_tag="VGD55_12100"
     CDS             complement(2442..2768)
                     /locus_tag="VGD55_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871134.1"
                     /translation="MGGAPPVSRAGFTSVAPDVEMNRLQTLTTAKGASAPRFLPAALG
                     LFLTSSCAQLNQLGFNQPDVTLIQQPSVRFVGATAVRANRGAADYPGGFDVARTGAEA
                     PLLADS"
     gene            complement(2798..>3260)
                     /locus_tag="VGD55_12105"
     CDS             complement(2798..>3260)
                     /locus_tag="VGD55_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871135.1"
                     /translation="GATDAGHDAAVTLYPLTTGDSCFDVLTVANVMDGCGIAPGSIVG
                     AALSVNYTMTATTGTLTVGTMGAEGAGPIANNTGTLTRDGDTSDSTMPLCTWHQMDTA
                     QVTLTGSNAFTLTVTEVETNLSAACGATAPNCTSTWTWTMKKGSKTPPTCQ"
BASE COUNT          614 a         1046 c         1057 g          543 t
ORIGIN      
        1 cggcgggggg gccgggacgg ccggcgcgac tgctgggacg aataggggtg gcggcggggg
       61 ggccgggacg gccggcgcga ctgctgggac gaacgggggt ggtggcaaag ggggcggcgc
      121 cggatcgggc gcggggggcg cgagcgccgg gaccgtcgtc gccatccctc cgtacacgat
      181 ctccgtatgg gcgacgggga ccaatgacta ccaggcgccc gattcgatcg agactgacgg
      241 aacgcacatc tgggtgggtt accagaacaa gaccaccaag gacgggagtg acggcggtgc
      301 ggggttcgtg gccaacagca cggtggtcga atacaccttg gacggtacca ccgtcttgaa
      361 aacgttttca ctggcggggc acgtcgacgg ccttcgggtc gaccccacga acaagaacgt
      421 atgggtgacg tcaaatgagg acggcgcacc tcacctgttc atgattgacg ccaccaccca
      481 ggtggtgacc agctacgttc tcccttcgcc tcttccccac ggggggggcc tcgacgacat
      541 cgggttcgtc gatgggaaga tgttcgtggc cgcgtcggcg ccctccgcaa cgaaggctac
      601 tcagaaccga ccggccctct atcaggtcac catttcaggg accacagcga cgctcaccca
      661 ggcgctagcc ggtaacggat ccgcgacgga ctacaacgtg gccgatgccg gggtagccct
      721 caatctgacc gatccagact ccatcacaat cagccccacc ggggacctcg tgctcgtctc
      781 ccagggggac aatcaggtgc tgttcgtgac ccaccccgga tcgacgccgg ccatcaagtc
      841 gttgaccgtt gccacccagc tcgatgatac cgtgtggacc acgaacacga agggccagtt
      901 gctcgtggtc gacggaaagg caaatatcat ctactccgtt agacggagtg acgggtggac
      961 ggtgggcatc gtctataccg agacgcctga cgattctaca atccccaaca tcctgggcac
     1021 cctcgatctc acgacgggaa acatccagcc gatcgccgtg ggctttggca aggcgaccgg
     1081 cctcctcttc ctgccgcagt aattcccgtt ccacagaggt gaactgccac gggatccttg
     1141 gacgctgttt gcctcagcgc aatgtagtga ggcctgacgg ctgcgcgctc tccacttgtc
     1201 ggtcggttgc aaatactacc ccatcgtctt ccactacggg tctgaaaccg tttcgaacgc
     1261 ggcggtccgc ctccggggga agtcgtcgtg ggtcaacacc gtcatgttcg acaagaatcc
     1321 gaagatggcg ttcgacatct cgttcgacca atacgacacg caccagaagt tccacgggat
     1381 ctccacgatc cacttcgaga tggcgcgcga tgaatggagt ttcctcagtg agcgcgtcgg
     1441 caacaactgg ttccgcgaga tcggtctcac ggcccttgct cgaacagcgc caccatcacc
     1501 gtcaacggcc agttctacgg gctctacttg gcggaggaag gcttcacgaa gtcactgctc
     1561 gctcagtttc tccccgggaa cacagacggc gaccttctca agggggggac ggaggcgcac
     1621 acgaacatcg gaacgacgaa ctggacgaag gtgcaggcac tcgacagcgc gactgacatg
     1681 ggcaccttgc ggacgctggt cgacgtgccg aacacggtgc tcgaatcggg agccgaagcc
     1741 gtcgtcgaag acgccgacgg ctactacggc ggttttcaca acttctggat atacgatcag
     1801 gggaccgcgg gatacgtctg gttgctggat cacaccgact ccgctctcga gtgggtcgag
     1861 atgttcaccc cgctcggtta caaggagcat ccgatctact ggtggatggg tcggcccctg
     1921 cccgatccgc ccgccaagaa ctacttgctg gtcatcaacg atccgaccgc gcgcacccag
     1981 tatgtgaacg cgatcgcgac gcaggtcgcg aagtggaacg ccaccgagat cgtcggctgg
     2041 atcgacacct ggtcgaagca gatcgccgac gcggttgggc aggacacgca caagtgggcg
     2101 accaccgatc agttctggat ggcgatcgcc gcgatgaggg acgaggccca gaatcggccg
     2161 cagtacctga acggcttctt ggcctgtgag aatggcgacc cgtctcaggc gtctgaccag
     2221 gacggggatg gggttgcgtg gtgcaacgac tgcgacgact caaacgcgtc tgtccacccc
     2281 ggcgcgccgg aagtctgcgg caaccacgtc gacgacaact gcaacggcgt ggttgacgag
     2341 aactgccccg gtgaaaagcc cggctatccg ggcgagccgg acggcggggt tccgccctca
     2401 cctggcgcgg acgcgggcgt cttgaccgac gctggccgcg gctaagagtc ggctaagagc
     2461 ggcgcctcag cgccggtgcg agccacgtca aagccgcccg ggtaatctgc cgcgccgcga
     2521 ttggcacgca ccgcggtcgc gcccacgaac cgcaccgacg gctgctggat gagggtcacg
     2581 tccggctgat tgaatcccag ctgattgagc tgcgcgcacg aagacgtgag gaacagcccc
     2641 agcgctgccg gcaggaaacg cggcgccgaa gctcctttcg ccgtggtcag ggtttggagc
     2701 cgattcatct cgacgtcagg agcgaccgag gtgaaaccgg cgcgtgatac tggcggcgca
     2761 ccgcccacca gcatcacgtc ggatcgatca cgccgcccta ctgacaggtc ggcggggtct
     2821 tcgacccctt cttcatcgtc caggtccagg tggacgtgca gttcggcgcc gtcgcgccac
     2881 aagccgccga aaggttcgtc tcgacctcgg tcaccgtgag cgtgaacgcg ttcgagcccg
     2941 tgagcgtcac ttgcgccgtg tccatctggt gccaggtgca cagcggcatc gtcgagtccg
     3001 acgtgtcgcc gtcgcgcgtg agcgttccgg tgttgttggc gatcgggccg gcgccctcag
     3061 cgcccatcgt gccgaccgtc agcgtcccgg tcgtcgccgt catcgtatag ttcacggata
     3121 gggcggcgcc gacgatactg cccggggcga tgccgcaccc gtccatcacg ttcgccaccg
     3181 tcagcacgtc gaagcagctg tcgccggtag tgagcgggta caacgtcacc gccgcgtcgt
     3241 ggccggcgtc ggtcgcgccg
//
LOCUS       DASWVF010000687         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_324807, whole genome shotgun sequence.
ACCESSION   DASWVF010000687 DASWVF010000000
VERSION     DASWVF010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324807"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGD55_12110"
     CDS             complement(<1..193)
                     /locus_tag="VGD55_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0871136.1"
                     /translation="MPVPGELLFVAPRRAKPPRHLADLSPVERRTAVGELGQPLFRAS
                     QLSNHYFSRLVDDPAAMTDV"
     gene            334..1377
                     /locus_tag="VGD55_12115"
     CDS             334..1377
                     /locus_tag="VGD55_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0871137.1"
                     /translation="MGLGHDISREFIAVPDGAKGQLLFKWPDHQIRRFTSAIVDVDEL
                     AIFISQGRVIGLLQPGRHRIDASELPFLGDVADKLSGGDAYDSELFFVSTHEFPDLTF
                     GGPVDSVRDPETRLVVQLRAYGDFALQVVDPSSLITKLTGTVDLADPAAVVAWAADQV
                     LKAVRTVVVGHVVNDQWPVLGLAGRTPDIEAGTVLAANQALATYGIRVTRFGNVTVSV
                     SDEDAEQLRTLARDTAYTALTGSFGTYAQGEALLGAGKGMANGQGGGNEALGIASLGL
                     GAGVLGAFAGAQQAVAPAAAPAPATSAAPASAAPTAIAAPVVAPAFCSHCGHALAAGA
                     LFCSACGTAVPAS"
BASE COUNT          252 a          544 c          493 g          251 t
ORIGIN      
        1 ggacgtcggt catcgccgcc gggtcgtcga ccagccgact gaagtagtgg ttggacagct
       61 gagacgcccg aaatagcggc tgaccgagct cgccgaccgc cgtccgccgc tcgacaggcg
      121 acagatcggc cagatgacgc ggcggcttgg ctcggcgcgg ggcgacgaag agcagttctc
      181 cggggacagg catggtgcgt ccagtctccc ctacctcaag gaccgccgcc tcaaggaccg
      241 ccgcctcaag gaccaccgcc tcaaggacca ctgcgcgcac accgacatta aggaagaacg
      301 gcaggtatcc gccccacgat cacaggagtg ttgatgggtc tcggccacga catcagtcgc
      361 gagttcatcg ccgtgcccga cggcgccaaa ggtcagctgc tcttcaagtg gcccgaccac
      421 caaatccgtc gtttcaccag cgcaatcgtg gacgtcgacg aactggcgat cttcatttcg
      481 cagggccgcg tcatcgggct cctgcaaccc ggtcggcacc gaatcgacgc gagcgagttg
      541 cccttccttg gcgatgtcgc cgacaagctg agtggcggag acgcctacga ttccgagctc
      601 ttctttgtca gcacgcatga attcccagat ctcaccttcg gggggcccgt cgactcggtg
      661 cgcgacccgg agaccaggct ggtcgtgcag ctgcgggcgt acggcgactt cgccctccaa
      721 gtcgtcgacc cgtcctcgct catcaccaag ctcaccggaa ctgtcgatct cgccgacccc
      781 gctgccgtcg tcgcgtgggc ggccgatcag gttctcaagg ccgtccgcac tgtcgtggtc
      841 ggtcacgtgg tgaacgacca gtggccggtg ctaggtctgg ccggtcgcac ccccgacatc
      901 gaggccggca ccgtactggc cgcgaaccag gcgctcgcca cttacggcat ccgggtcacc
      961 cggttcggca atgtcaccgt cagcgtgtcc gatgaggacg ccgagcagct acggacgcta
     1021 gcccgcgaca ccgcctacac ggcgttgacc ggctcgttcg gcacctacgc gcagggcgag
     1081 gcgctgcttg gtgccggtaa ggggatggcc aacggtcaag gcggcgggaa cgaagctctc
     1141 gggatcgcct cgctcgggct cggcgctggc gtgctcggtg cgttcgcagg agcccagcag
     1201 gcggtcgccc ccgcggccgc gcctgcgccg gctacatcgg ctgcgcctgc atccgctgcg
     1261 cctaccgcta tagcggcccc cgtcgtcgcg cccgcgttct gctcgcactg cgggcatgcg
     1321 ctcgccgcgg gcgccctgtt ctgctcggcc tgcggcaccg ccgtccccgc ctcgtagtga
     1381 gcatcgcgtg agcttgttca agaagcgcga cgacgaggca acacgactgc agtcgttcgt
     1441 gctggatcca ccagcgacgg gcacgcccag cgccatcggc atcgccggtg acgtcgacat
     1501 cactgccatt caggtagtcg acgcgacgtt tgatccgaaa
//
LOCUS       DASWVF010000688         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_223574, whole genome shotgun sequence.
ACCESSION   DASWVF010000688 DASWVF010000000
VERSION     DASWVF010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223574"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..203)
                     /locus_tag="VGD55_12120"
     CDS             complement(63..203)
                     /locus_tag="VGD55_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871138.1"
                     /translation="MTTGALEAGPADESEPVAADDDAAGVAGADAGDEAEVAGGEPVE
                     VA"
     gene            403..1257
                     /locus_tag="VGD55_12125"
     CDS             403..1257
                     /locus_tag="VGD55_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871139.1"
                     /translation="MPAALTGASPAITKVRFATASDGWAFGPQLWSTHDGGHTWSSTA
                     TPGPVSDVEASGGEAYALVASCGTPPCVQGDRLLKTPVSTDGWTAISGPALPDGAGSV
                     SPHGNVVWVVVNGGGSSTFDMITGGTSWHRLANPCTSAGADLSLTGVAPVSASAMFLL
                     CAGNPGAGSEDKIVLFSHDGGAHASATSAAPSRGGIATGIAAASTAVVAVSAASGASF
                     VYRSADGGNTWGTALTQGDGGVGYYDVGFTTSTQGVAIYGNPAVGNAATSLLMTYDAG
                     ATWKPVTF"
     gene            1236..>1774
                     /locus_tag="VGD55_12130"
     CDS             1236..>1774
                     /locus_tag="VGD55_12130"
                     /inference="COORDINATES: protein motif:HMM:NF041024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEY0871140.1"
                     /translation="METGHLLTLSRTLDIGPQRLERWLRGFEERHGDVEWSASGAALN
                     VVAADGATAVLVAPYPPLPDGWADGDAVAAMVEHALRRRVIGVLLVRLGGYAAGVFDG
                     DEVVASKVGSRQVHGRSAAGGWSQHRFARRREGQVKVALEAAINTAAAVVVPHASRLA
                     AVVTGGDRPALRTVLADARL"
BASE COUNT          237 a          586 c          647 g          304 t
ORIGIN      
        1 gcgcgggctg ccgtcatcac actgggcgct cttcttgcta tggctggctg ttcgagcagc
       61 catcacgcga cctcaaccgg ttcacctccg gccacctcgg cctcgtcgcc ggcgtctgca
      121 cccgcgaccc ccgcggcgtc gtcgtcggcg gccacgggct cgctctcatc tgcaggccca
      181 gcctcgagtg ccccagtcgt cacgccgcct ccaacggcga cacccgctgg actggccggg
      241 ttccgaccgg cgtccgtcac attcgtctcg gcgacgcaag ggttcgtgct cggcgctcag
      301 acatgcgcgg gtgcagcctg tggtcagggt tgctcctctg gaacgtgcgc caccctcgca
      361 acgtcggtcg acgcggggaa gacgtggacc aacgtcggga tagtgcccgc tgcgttgacg
      421 ggcgcgagcc cggcgattac caaggtgcgg ttcgccacgg cgtccgacgg ctgggctttc
      481 ggcccgcaac tgtggtcgac ccacgacggc gggcacacgt ggagcagcac cgcgacgcct
      541 ggaccggtga gcgacgtcga ggcttccgga ggtgaggcct atgcgctcgt cgcgagctgc
      601 gggacgccgc cgtgcgttca gggtgatcgg ctcttgaaga cgcccgtctc gacggacgga
      661 tggacggcca tctccggtcc ggcgcttccc gacggcgctg gatctgtctc gccgcacgga
      721 aacgtcgtgt gggtcgtcgt caacggcggc gggtcgagca cgttcgacat gatcaccggc
      781 ggcacgagct ggcaccggct ggcgaatccc tgcacatcgg ccggggctga cctgagcctg
      841 accggagtcg cgccagtgtc ggcgtccgcc atgttcttgt tgtgtgctgg caatccgggc
      901 gccggcagtg aagacaagat tgtgctgttc tcccacgacg gtggtgcgca cgcttctgca
      961 acgagcgccg caccgtcgcg cggcgggatc gccaccggca tcgcggcggc aagcaccgcg
     1021 gtggttgcag tgtcggcggc gtcgggtgcg agcttcgtct accgctccgc cgatggcggt
     1081 aacacctggg gcactgcgct gacgcagggt gacggcggcg tcggctacta cgacgtcggg
     1141 ttcacgacct cgacgcaagg tgtcgccatc tacggcaatc cggcggtcgg caatgcggca
     1201 acgagcctgc tcatgaccta cgacgccgga gcgacgtgga aaccggtcac cttctgacgc
     1261 tgtcacgcac cctcgacatc ggtccgcagc ggctggagcg ttggctgcgc ggatttgagg
     1321 agaggcatgg ggacgtcgag tggtccgcgt ccggtgctgc gctgaacgtt gtcgctgccg
     1381 acggtgcgac agctgtactc gtcgctccgt accctccgct gccggacggc tgggccgatg
     1441 gcgacgcggt ggctgcgatg gtcgagcacg ccttgcgccg gcgggtcatc ggcgtgttgc
     1501 ttgtgcgcct tggaggctac gcggccgggg tattcgacgg cgatgaggtg gtggcgtcga
     1561 aggtgggctc gcgacaggtg cacggcaggt cggcggcggg cgggtggtcg cagcatcgct
     1621 tcgcgcgccg tcgcgaaggt caggtgaagg tcgcgttgga ggcggcgatc aatactgcgg
     1681 cggctgtcgt ggtgccgcac gcgtcgcgac tcgcggcagt cgtcaccggc ggtgatcgcc
     1741 cggcgctgcg taccgtgctc gctgacgcac ggct
//
LOCUS       DASWVF010000689         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_223577, whole genome shotgun sequence.
ACCESSION   DASWVF010000689 DASWVF010000000
VERSION     DASWVF010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223577"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /gene="pilM"
                     /locus_tag="VGD55_12135"
     CDS             <1..1048
                     /gene="pilM"
                     /locus_tag="VGD55_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY0871141.1"
                     /translation="VGLDIGTSAVRAAELSVGRGGYTLERFGQVALPVGAVVDGEVLD
                     RDAVTAALKVLWAQTKFSTKRVVLGVANQKVVVRQVDLPWMEPKELRNALAFQVQDFI
                     PIPVDQAIMDFHPLEEFTNDSGNRMLRVLLVAAGREMVGSCLDAVQHAGLTPVMVDLT
                     SFAIIRASTHADALGMDTQAEALIDIGARVTNIAVHQGGQPKFVRVLLMGGADITEAV
                     AERMGIPLEQAEALKQQIGIPATAAERDENPAGRVIDGIAASFVEEIRGSLDYYLASP
                     AAVPIRRIVVSGGGSRLENLVSRLAAVTRLPVECANPLASVRIGNTKLSTEQLGHIER
                     LAAVPVGLAMGVAS"
     gene            1045..1728
                     /locus_tag="VGD55_12140"
     CDS             1045..1728
                     /locus_tag="VGD55_12140"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY0871142.1"
                     /translation="MSTTTTSTRVVELPRVNLLPPEIFEKRQLQRAQAGLGVVVIAAL
                     VGVGLVYVSGGSKVTDAKSRLAASQATQTNLQSQLNQLNFVTAEAAQAQAAKSMLTQA
                     TASTIPFSTYLADLSLLTPKNVWFTAITLTSSVSPGTITDPTTAPATVGNVTFSGQAL
                     AHNDVATWLDNAAKEGGFADPYFSSSTESVIPGTGTAVGSKPKTWVTFSSTVNLTSAA
                     LCGAQPGKC"
     gene            1730..2512
                     /locus_tag="VGD55_12145"
     CDS             1730..2512
                     /locus_tag="VGD55_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871143.1"
                     /translation="MSRIQQWIAGTVVAVLVIVAAGWFLAISPQKHKVSKLDSSATQQ
                     EQANSGLRTKLATLKVAAAAVPSEQAEVNAITQKIPSDPAMPSYVRALTVIAQKYSVE
                     LVSIAPGTPAAVTIAVKAAAPAAAAAATPSAAPAAAATPAVAVAPVSLQAISVSLTVQ
                     GGYWQIQQFTDALEKLARTTVVSAISLAPAGALKKVTLPSSAPTPPAYKSLGATITLS
                     VFMNSSDTFDIPAAPAQASGASVPGAGAASAAPAQPSASASN"
     gene            2524..3252
                     /locus_tag="VGD55_12150"
     CDS             2524..3252
                     /locus_tag="VGD55_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871144.1"
                     /translation="MSESSLSPTGSSQKNQRLALVAVVGVAVVGVIVYLLLFSGGSSS
                     SPAAARVTPHVAASAPASASPSAIQAVPAAFTGAVGRDPFQPALTPPPVAATASSSAA
                     AASAAAASASKAAAASPSPTLIVIPTLTLQPSPTVTVTASPSPTATVTVTPSPTGLPT
                     AGNSITLTLVRADGSGTVDVTVTNGATTTPYNGVAPGQVFGTYFKLVSILSDSSTTPT
                     TYGADFEYGDQFVQLAKGQSATLG"
     gene            3345..4523
                     /gene="aroC"
                     /locus_tag="VGD55_12155"
     CDS             3345..4523
                     /gene="aroC"
                     /locus_tag="VGD55_12155"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041834988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0871145.1"
                     /translation="MLRWLTAGESHGPALVAILEGLPAGVHTSTAEVADALARRRAGY
                     GRGARMSFERDEIDFLGGIRHGVTMGGPVAIRVGNSEWPKWEKVMAPDPVDEDELADL
                     ARNAPLTRPRPGHADLVGMQKYALTDVRPILERASARETAARVALGEVASSFLRQALD
                     VEVLSHVVSIGQVSAPAGLVPDPADRDRIDGDPVRCFDPATSDAMVAEIDAARKDGDT
                     LGGVVEVVVHGLPPGLGSHVHWDRRLDSRLAGALMGIQAIKGVEVGDGFESARRRGSY
                     AHDEIENTALGVRRRTNRAGGTEGGMSTGEVLRVRAAMKPISTVPRALDTIDVATGEA
                     AKAINQRSDVCAVPAAGVVAEAMVALVVADAVLEKFGGDSVGEVRRNVRGYLESLVVS
                     "
     gene            4534..5034
                     /locus_tag="VGD55_12160"
     CDS             4534..5034
                     /locus_tag="VGD55_12160"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0871146.1"
                     /translation="MAPLVVLVGPPGAGKSTVGRLLASRFGTTYRDTDLDVEKVAGMK
                     VADVFFEYGEDHFRELERVAVADALSEHDGVLVLGGGAILDPTTRALLHDQRVVYIDV
                     DLADATKRVGFNRDRPLLLGNPRAQLHALMEARRPLYEEVAMTTVNSSGKTVAAVTAE
                     VAEVIR"
     gene            5031..>5736
                     /locus_tag="VGD55_12165"
     CDS             5031..>5736
                     /locus_tag="VGD55_12165"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEY0871147.1"
                     /translation="MTDRIVVDGDEPYDVFVGVGALGELGRLVPPDVRRIALVHQGAV
                     ALLVDDIEKALSAHGDVVRIEVPDGEAAKTAAVTVAAWDTLAAHGFTRTDVVIGIGGG
                     AVTDLAGFIAATWLRGVRVLQVPTTLLAMVDAAIGGKTGINIAAGKNLVGAFHPPAGV
                     ICDLSTLDSLPHADYVAGLAEVVKSGFIADPEILELIQSDPVGASTPAGPHTAALVVR
                     TIAVKAAVVGADLREAA"
BASE COUNT          944 a         1897 c         1878 g         1017 t
ORIGIN      
        1 cgtcggtctc gatatcggga cgtctgcggt acgcgccgcc gagctttcgg ttggccgcgg
       61 cggttatacc ctcgagcgct tcggccaggt agccctgccc gtcggcgcag tcgtcgatgg
      121 cgaggtcctc gatcgggacg cagtcaccgc agcgctcaag gtgctgtggg ctcagaccaa
      181 gttctcgacc aagcgagttg tcctcggcgt tgcgaaccag aaagtcgtcg tgcgtcaggt
      241 cgacctgccg tggatggagc cgaaggagct gcgcaacgcg ctcgccttcc aggttcagga
      301 cttcatcccg atcccggtcg accaggccat catggacttc caccccctcg aagagttcac
      361 caacgactcc ggcaaccgca tgctgcgggt gctgctcgtc gctgccggcc gcgagatggt
      421 cggttcgtgt ctcgatgccg tgcagcacgc agggctcacg ccggtcatgg tcgacctgac
      481 gtccttcgcg atcatccgcg catcgactca cgcagacgca ctcggcatgg acacccaagc
      541 tgaggcgctc attgacatcg gcgcccgcgt gacgaacatc gccgttcacc aaggtggtca
      601 gcccaagttc gtccgtgtcc tcctgatggg tggcgctgac atcaccgagg ctgtcgccga
      661 gcggatggga atccccttgg agcaggccga ggcgctgaag cagcagatcg gcattcccgc
      721 gactgccgca gagcgcgacg agaacccagc aggtcgtgtc atcgacggga tcgcagcgtc
      781 gttcgtcgaa gagatccgcg ggtcgctcga ctactacctc gcgtcacctg ccgccgtccc
      841 gattcgccgg atcgtcgtat ccggtggagg gtcgcggttg gagaacctgg tctcacggtt
      901 ggccgccgtt acccggctgc ccgtcgagtg cgcgaatcca ctcgcttccg tgcgaatcgg
      961 caacaccaaa ctgtcgaccg aacagctcgg tcacatcgag cgcctggcgg cggtgcccgt
     1021 cgggctggca atgggagtcg cctcgtgagc acgaccacga cgtccaccag ggtggtcgaa
     1081 ctgcctcggg tgaacctcct tccaccggag atcttcgaga agcgtcaact gcagcgggcc
     1141 caagcgggcc tcggcgttgt cgtcatcgcg gcgctcgtcg gcgtgggcct tgtctacgtc
     1201 agcggcggtt ccaaggtcac tgacgcgaag tcacggctcg cggcatcgca ggccacccaa
     1261 accaatctcc agtcacaact gaaccaactc aactttgtta cggccgaagc ggctcaggcg
     1321 caggcagcga agagcatgtt gacgcaggca actgcctcca cgattccgtt ctcgacgtac
     1381 cttgccgacc tcagcctgct gacgccgaag aacgtctggt tcacggccat cactcttacg
     1441 agttcggtgt cgccaggaac catcaccgac ccgacaaccg ctccagcgac agttggcaac
     1501 gtgacgtttt ccggtcaggc gctcgcgcat aacgacgtcg cgacctggct cgacaacgcg
     1561 gcgaaggaag gcggcttcgc cgatccctac ttcagctcgt ccaccgagag cgtgattccg
     1621 ggtaccggaa cggctgtcgg ttccaagccg aagacttggg tcacgttcag ctcgacggtc
     1681 aacctcacat ccgcggcgct gtgcggcgcc cagccgggaa agtgctgaca tgtctcgaat
     1741 ccaacagtgg attgccggca ccgtagtcgc cgtacttgtc atcgtggcgg cgggttggtt
     1801 cctcgcgatc tcaccccaaa agcacaaggt ctcgaagctt gactcgtcgg ccacccagca
     1861 ggaacaagcc aacagcgggt tgcgtaccaa gctcgcgacg ctgaaggtgg cggcggcggc
     1921 ggtaccgagt gagcaggccg aggtcaatgc gatcacgcag aagatcccga gcgatccggc
     1981 gatgccgagc tacgtccgcg ctctcaccgt gattgcgcaa aagtacagcg tcgagctggt
     2041 ctcgatcgcg ccaggtacgc cggcggctgt caccatcgct gtgaaggctg cggctcccgc
     2101 cgctgctgct gcggcgacgc cttctgcggc accggctgct gcagcgacgc ccgctgtcgc
     2161 tgtcgcgccg gttagtctgc aggcgatctc ggtgtcactc accgtgcaag gcggttactg
     2221 gcagattcag cagttcaccg acgctttgga gaagctggcg cgtacaactg tcgtcagcgc
     2281 tatctccctc gctccggctg gagcgttgaa gaaagtgacc ctgccgagct cagcgccgac
     2341 accgcctgcc tacaagagct tgggcgccac gatcaccctc agcgtcttca tgaactcaag
     2401 cgacaccttc gacatccccg ctgcgccggc ccaggcgtcg ggcgcgtcgg tcccaggcgc
     2461 aggggctgca tctgcagcgc cggcgcagcc gtccgctagc gcgagtaact aggagacccg
     2521 gatatgagcg agtcctcact gtcgccgact gggtcgtcgc agaagaatca gcgcctcgcg
     2581 ctggtcgcgg tcgtcggagt ggccgtggtc ggagtcatcg tctacctgct gctgttctcc
     2641 ggcggatcgt cgtcgagtcc ggccgcagcg cgagtgacgc cgcatgttgc ggcgtcggca
     2701 cctgcctcgg cgagcccatc cgcgatccag gcggtccccg cggcctttac cggcgcggtc
     2761 gggcgcgacc cgttccagcc tgcgctgaca ccaccgccgg tagcggccac tgcgtcgtcg
     2821 tctgcggcgg cagcctccgc cgcagctgcg tcggcttcca aagctgccgc tgcgtcgccg
     2881 tccccgacgt tgatcgtcat tccgacgctg acgctgcagc catcgccgac ggtcaccgtc
     2941 actgcgtcac cgtcgccgac cgcaacggtg acggtcacac cgtcgccgac tggtctaccg
     3001 actgctggca actcgatcac gttgacgctc gtgagggctg atggttccgg cacggtcgac
     3061 gtgaccgtga cgaacggagc gaccaccacg ccctacaacg gcgttgcacc gggacaggtg
     3121 ttcggcacct acttcaagct ggtgtcgatc ctgtctgaca gctcgaccac acccaccacc
     3181 tacggcgccg acttcgaata cggcgaccag ttcgtccagc tagccaaggg gcagtcggcg
     3241 acgctcggct aactccaccc cgcttgacct ttggccccga ccggtttcga ccggccgggg
     3301 ccgagtcaat tctccggtaa cgcagttatg ggagactcgc ggtcatgctg cggtggctga
     3361 cagccggtga gtcgcatggt cctgcactcg tcgcgatcct cgagggactt cccgccggcg
     3421 tgcacacatc gaccgccgag gtcgccgacg cgctcgctcg gcgccgcgcc ggctacgggc
     3481 ggggtgcccg catgtcgttc gagcgcgatg agatcgactt cctcggtggc atcaggcacg
     3541 gcgtcacgat gggcggcccg gtggcgatcc gggtcggcaa cagcgagtgg ccgaagtggg
     3601 agaaggtcat ggctcccgac ccggtcgacg aagacgagct ggccgatctc gcgcggaacg
     3661 cgccgctgac acgtcctcgc cctggccacg ccgacctggt cggtatgcag aaatacgcac
     3721 tgaccgacgt ccggcccatt cttgaaaggg cgagcgcccg cgagaccgcg gcacgggttg
     3781 ctcttggcga ggtcgcgtcg agcttcttgc gtcaggcctt ggacgtcgag gtgctgagcc
     3841 acgtcgtatc gatcggtcag gtgagcgcac cggcggggct cgttcccgac cccgccgacc
     3901 gtgaccgcat cgacggcgat ccggtgcggt gcttcgaccc cgcgaccagc gacgccatgg
     3961 tggccgagat cgacgcagct cgaaaggacg gcgacaccct tggcggcgtc gtcgaagtcg
     4021 tggtgcacgg cttgcctccg gggcttggca gccatgtgca ttgggatcgg cggctcgact
     4081 cgcgcctcgc gggcgcgttg atggggatcc aggctatcaa gggtgtcgag gtcggcgacg
     4141 ggttcgagtc ggctcggcga cgcggctcgt acgcacacga cgagatcgag aacaccgctc
     4201 ttggcgtgcg acgccgaacc aaccgcgcgg gcggcaccga aggtggcatg tcgacggggg
     4261 aggtgctgcg cgttcgcgca gctatgaagc ccatctcgac ggtgccacgc gcactcgaca
     4321 ccatcgacgt tgcaaccggt gaggccgcaa aggcgatcaa tcagcgcagt gacgtctgtg
     4381 ccgtacctgc tgcgggtgtc gtggccgagg cgatggttgc gctcgtggtc gcggacgccg
     4441 tcttggagaa gttcggcggc gactccgtcg gcgaggtacg tcgcaacgtg cgcggctacc
     4501 tcgagagcct cgtcgtctcg tgacacctcg gtgatggcgc ctctcgtggt ccttgttggc
     4561 cctcccggcg ctggcaagag cacggtcggg cgattgctcg ccagccgttt cggcaccaca
     4621 tatcgcgaca ccgatctcga tgtcgagaaa gtcgcgggca tgaaggtcgc tgacgtgttc
     4681 ttcgaatacg gcgaggatca ttttcgagaa cttgagcgcg tggcggtcgc cgatgcatta
     4741 agcgagcacg acggcgtgct cgtcctcggc ggcggtgcga ttctcgatcc gaccactcgc
     4801 gcgctgttgc acgaccagcg cgtggtctac atcgacgtcg accttgccga tgccacgaag
     4861 cgggtggggt tcaaccgcga tcgtcctttg ctgctaggca atccgcgggc acagctccac
     4921 gcgttgatgg aagccagacg acccttgtac gaagaggtcg cgatgacgac cgtcaacagc
     4981 tcgggcaaga cggtcgcggc cgtcaccgcc gaagtggccg aggtgatcag atgaccgatc
     5041 gcatcgtggt cgacggcgac gagccctacg atgtcttcgt cggcgttggc gcgctcggcg
     5101 agttgggtcg gttggttcct ccggacgtgc gccggattgc cttggtgcat cagggcgcgg
     5161 tcgcgctctt ggtcgacgac atcgagaaag ccttgtcggc tcatggcgac gtcgtacgca
     5221 tcgaggttcc cgatggtgag gcggcaaaga ccgccgcggt tacggtcgcc gcgtgggaca
     5281 ccctcgcagc ccacgggttc acgagaacag atgtcgtgat cggtatcggt gggggagcgg
     5341 tcaccgatct ggccggcttc atcgccgcca cctggcttcg cggtgtccgg gtccttcagg
     5401 tacccacgac gttgctcgca atggtcgacg cggccatcgg cgggaaaacc ggtatcaaca
     5461 ttgcggccgg gaagaacttg gtcggcgcgt tccatccgcc ggccggagtc atctgcgatc
     5521 tgagcacgct cgactcgctt ccgcatgccg actacgtcgc cggcctggcc gaggtcgtca
     5581 agtcgggatt cattgctgat ccggaaatcc tcgagctgat ccagtcggat cctgttggtg
     5641 catcgacgcc ggcggggccg cacacggctg ccctcgtcgt ccggaccatt gcagtgaagg
     5701 ctgccgtggt cggtgctgac ctgcgtgaag cggcgg
//
LOCUS       DASWVF010000690         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_146802, whole genome shotgun sequence.
ACCESSION   DASWVF010000690 DASWVF010000000
VERSION     DASWVF010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146802"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VGD55_12170"
     CDS             <1..557
                     /locus_tag="VGD55_12170"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0871148.1"
                     /translation="QIVFEIDGALVDLVRARLPWRRNRRLRIRVGDARAGLAAIADAS
                     CDLVVRDAFRDAVVPVNLRTVECAIRVKGILRPGGVYVMNVADRAPFATTGVELATVA
                     EAFSDVAVISEPGVLRGRRHGNLVIVASNEPIPVSALARRVADGGVQGRVRDRVQSME
                     IARGHRALRDADVTGTEFGSAQLQ"
     gene            complement(566..>1673)
                     /locus_tag="VGD55_12175"
     CDS             complement(566..>1673)
                     /locus_tag="VGD55_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0871149.1"
                     /translation="SKADDALHHMRAGEVTSSAVNAAHNDMVAATICLLVGLLLVLVA
                     LGLWLRHDVLRPLEVLRGRMADIADGDGDLTRRLAFHRDDEIGSLAGEFDRFVTGMQS
                     LVGKVAGTAAGLATSAASLSATSEQIASSAHDATRRAESASSAAEQVSSNVASVAGAA
                     EEMGSSIREIAHNAHRAAGVAIDAVTMADTTNAAISRLGESSLDIGSVVKLITEIAQQ
                     TNLLALNATIEAARAGEAGKGFAVVADEVKDLAQETARATDDISRRIESVRNDSRDAI
                     AAIEAIRVVISQISDYQVTIASAVEEQSATTSEMGRGFTEVATGSAEIARSIAGVASA
                     SSTTLTSVASARVTTDELRKVADELKTLVSRFTY"
BASE COUNT          263 a          539 c          557 g          314 t
ORIGIN      
        1 cacagatcgt gttcgagatt gacggtgcgc ttgtcgatct cgttcgagcg cggctgccgt
       61 ggcgccgcaa ccggaggcta cgcatccgag tcggtgacgc gcgcgccgga ctcgcagcaa
      121 tcgccgatgc gtcgtgcgac ctcgtggtcc gcgatgcgtt tcgcgacgcc gtggtgccgg
      181 tgaacttgcg caccgtcgag tgcgcgatac gcgtgaaagg aatccttcgc cccggtggcg
      241 tctacgtcat gaacgtcgca gatcgcgcgc cctttgcgac aaccggcgtc gagttggcaa
      301 cggtggccga ggctttcagc gacgtggcgg tgatctcaga gccgggagtc cttcgcggcc
      361 gtcggcacgg caacctcgtc atcgtcgcgt cgaacgagcc aatcccagtc agcgccctcg
      421 cgcgtcgggt cgccgatggc ggcgtgcaag gcagggtgcg ggatcgggtc cagtcgatgg
      481 aaatcgcgcg cggacatcgc gcactgcgtg acgccgacgt cactggtact gagttcggca
      541 gtgctcagct tcaatagatg cgcggtcagt atgtgaagcg cgagaccagc gtcttgagct
      601 cgtcggcaac tttgcgcagt tcgtcggtcg tgacccgcgc tgaggcgaca ctcgtcaatg
      661 tcgtcgatga ggcgctcgcg acgccggcaa tcgaccgggc gatctcggcg ctgccagtgg
      721 ccacctcggt aaatccgcgg cccatctcac ttgtcgtcgc tgactgttcc tcgacggccg
      781 acgcgatggt cacttggtag tcgctgatct gactgatgac gacccgaatc gcttcgatgg
      841 ccgcaatcgc gtcacgcgaa tcgttgcgca ccgactcgat gcggcgagag atgtcgtcgg
      901 tagcccgtgc ggtttcctgc gcgagatcct tcacctcgtc ggcgactact gcgaatccct
      961 tgccagcttc gccggctcgc gctgcttcga tcgttgcgtt aagcgcgagc aggttcgtct
     1021 gctgcgcaat ctcggtgatg agcttgacga cgctgccgat gtcgagcgac gactcgccca
     1081 gcctcgagat agcagcattg gtcgtgtcgg ccatggtcac tgcgtcgatg gcgacaccgg
     1141 ccgcgcggtg tgcgttgtgc gcaatctcgc ggatcgacga tcccatttcc tcggcggcac
     1201 cggccacgct ggccacgttg ctgctgacct gctcggctgc gctcgaagcg ctttcggctc
     1261 gacgggtcgc gtcgtgtgcc gacgaggcga tttgctcgga cgtcgcggac aggctcgccg
     1321 cagacgtggc aagaccggcc gcggtacccg cgaccttgcc gacgagtgac tgcatgccgg
     1381 tgacgaagcg atcgaactca ccggccaggc tgccgatctc gtcgtcacga tgaaaagcga
     1441 gtcgccgggt caggtcaccg tcgccgtctg cgatgtcggc catccggccc cgcagcacct
     1501 caagtgggcg cagcacgtcg tgacgcagcc acaggccgag ggcaacgaga acgagcagca
     1561 atccgacgag caggcagatc gtcgcggcga ccatgtcgtt gtgtgcggcg ttcaccgcgc
     1621 tcgatgtgac ctcgccggcc cgcatgtggt gcagtgcgtc gtcagccttt gac
//
LOCUS       DASWVF010000691         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_324922, whole genome shotgun sequence.
ACCESSION   DASWVF010000691 DASWVF010000000
VERSION     DASWVF010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324922"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGD55_12180"
     CDS             complement(<1..211)
                     /locus_tag="VGD55_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY0871150.1"
                     /translation="MHTHELQVSNGSDLVTDLTDAVAGFCRGLGDGLLNVFVPHATAG
                     VALMETGSGSERDLAATIDRLWPPDV"
     gene            complement(198..1175)
                     /locus_tag="VGD55_12185"
     CDS             complement(198..1175)
                     /locus_tag="VGD55_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0871151.1"
                     /translation="MSSTAAEPGTIRTLEVPEGLEGERLDAALARLFGFSRTRAAELI
                     GQGRVQLDGSTPIKSDRVTAGSWLQVELPEVAAAGLAIVSTPVANLLIVYDDADIVVV
                     DKPVGVAAHPSPGWDGPTVVGALVAAGYRVSTSGAEERQGVVHRLDVGTSGLMVVAKS
                     EHAYTVLKQAFRDRTVDKTYHALVQGHPDPARGTIDGPIDRHPVNDYKFAVVADGKPS
                     ITHYETLEGHAMASLLEVKLETGRTHQIRVHMAALRHPCVGDLAYGADPTLAAKLGLT
                     RQWLHAVRLAFAHPGDGHWIEFTSTYPADLEHALQVVRAGVEGERRAHS"
     gene            complement(1172..1780)
                     /gene="lspA"
                     /locus_tag="VGD55_12190"
     CDS             complement(1172..1780)
                     /gene="lspA"
                     /locus_tag="VGD55_12190"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY0871152.1"
                     /translation="MQTTRGTSLNGPGPSTQPGSTLIVGRRRHLGVLFAVAVFVVAAD
                     VISKIIVVAKLSGRPPVRLLDGLLTLDYTRNAGAAFSIGTGATYLFGIVAIAVIAVIL
                     RTARRLFSRPWAVVLGLLLGGATGNLIDRLVRSPGVLRGHVVDWIQLPHFAVFNLADS
                     AITIGGVLAVLLALMGRQLDGTVARSEQAPKPSEPSPDGPNS"
     gene            complement(1753..>2135)
                     /locus_tag="VGD55_12195"
     CDS             complement(1753..>2135)
                     /locus_tag="VGD55_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY0871153.1"
                     /translation="PAIPDEPWTKKELTAIRIELEAEAQRLRGEIEIAESELADRLRD
                     GGDGAGDDQADAGTKTFEREHEMSLANNARDLLVQTEHALGRIEDGTYGRCENCGNPI
                     GKLRLQANPRATLCVSCKQREERR"
BASE COUNT          376 a          714 c          703 g          342 t
ORIGIN      
        1 ggacgtcggg gggccagagc cgatcgatcg tggcggcgag gtcgcgttca ctgccagagc
       61 cggtctccat caaggcaaca ccggcagtgg catgcggcac gaacacgttc agcagcccgt
      121 cacccagacc ccggcagaaa ccggccaccg cgtcggtcag gtcagtcacc aggtcggagc
      181 cgttgctgac ctgcagctca tgagtgtgca cgccgctccc cttcgactcc ggcgcgcacg
      241 acctgcagcg cgtgttcgag gtcggccgga taggtgctgg tgaactcgat ccagtgcccg
      301 tcaccggggt gggcgaaggc gagccgcacc gcatgcagcc attggcgggt cagaccgagc
      361 ttcgcggcaa gtgtggggtc ggcgccgtat gcgagatcac ccacgcacgg gtgccgaagc
      421 gcggccatgt gcacccggat ctggtgcgtg cgcccggtct cgagcttgac ctcgagcagg
      481 ctcgccatcg cgtgaccttc gagcgtctcg tagtgggtga tgctcggctt accgtcggcg
      541 acgacggcaa acttgtagtc gttaaccgga tggcgatcga tcggcccgtc gatcgtgccg
      601 cgcgccggat ctggatgtcc ctggacgagc gcgtggtacg tcttgtcgac cgtccggtct
      661 cgaaacgcct gcttgagcac cgtgtaggcg tgctcgctct tggccaccac catcagcccg
      721 ctggtgccga cgtcgaggcg gtgcacgacg ccctgccgct cctccgcacc ggaggtcgaa
      781 actcgatagc cagctgcgac gagtgccccg acgacagtcg ggccatccca acccgggctc
      841 ggatgcgccg cgacgccgac gggcttgtcg accacgacga tgtcagcgtc gtcatagacg
      901 atcagcagat tcgccaccgg cgtcgagacg atcgccagcc cagcagcggc cacttcaggc
      961 agttcgacct ggagccatga gccggccgtc acccggtcgg acttaatcgg tgtcgagccg
     1021 tcgagctgca cccggccctg cccgatcagc tcggcggcgc gggtacggga gaagccaaac
     1081 agccgggcaa gcgctgcgtc gagacgttcg ccttccagac cctcgggcac ctcgagagta
     1141 cgaatcgtgc cgggctcggc cgccgtactg ctcacgagtt gggcccgtcc gggctgggtt
     1201 cggatggctt gggcgcctgc tccgaacgag cgactgtgcc gtcgagttgg cggcccatca
     1261 aggcaagcag caccgcgagc acgccgccga tggtgatggc cgagtcagcc aagttgaaca
     1321 ccgcgaagtg cggcagctga atccagtcga cgacgtgacc acgcagaacc ccgggcgagc
     1381 gaacgagccg gtcgatcagg ttgccggtcg ccccaccgag cagcaacccg agcacaaccg
     1441 cccaaggcct gctgaaaagc cgccgggcgg tacgcaagat caccgcgatc accgcaatcg
     1501 ccacgatgcc gaacaggtaa gtcgcgccgg tgccaatgct gaacgcggct cctgcattac
     1561 gggtgtagtc caaggtcaac aggccatcga ggagccgaac cggaggccga ccggacaact
     1621 tggccaccac gatgatcttg gaaatgacat cggccgcgac gacgaacacc gcaacggcga
     1681 acaggacgcc gagatggcgt cgccgaccca cgatgagcgt tgaacccggc tgggtgctgg
     1741 gtcctggccc gtttagcgac gttcctcgcg ttgtttgcat gacacacaca gggtcgcacg
     1801 agggttggcc tgaagtcgaa gcttgccaat cggattgcca cagttctcac agcgaccgta
     1861 agtgccgtct tcgatgcggc cgagcgcgtg ttcggtctga accagcaggt cacgggcgtt
     1921 gttggccagc gacatctcgt gctcgcgttc gaacgtctta gtgccggcgt cggcctggtc
     1981 gtcgccagcg ccgtcgccgc catcgcgaag gcggtcagcc aactcggact cggcaatctc
     2041 gatctcgccg cgtaggcgct gcgcctcggc ttcgagctcg atgcgaatcg ccgtcagttc
     2101 cttcttcgtc catggctcgt ccggaatcgc cgggg
//
LOCUS       DASWVF010000692         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_452960, whole genome shotgun sequence.
ACCESSION   DASWVF010000692 DASWVF010000000
VERSION     DASWVF010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452960"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VGD55_12200"
     CDS             complement(<1..1328)
                     /locus_tag="VGD55_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0871154.1"
                     /translation="MSWLPAGTLMDRAATGLAATCARLLPKVYGSRVVLLVGSGDNGG
                     DALFAGALLAQRGAVVSAVLLGSMAHAGGLTALRTAGGRIVGADAMANAELVIDGIVG
                     IGGKGGLRDDAAAVVELIPADALVVAVDVPSGVDADTGEVEGRAIRADVTVTFGTWKP
                     GLLIDPGAEHAGAVELVDIGLGPMLPTASITALQAHDVETLLPAAEAESDKYSRGVVG
                     IVAGSATYTGAALLATGGALRAGAGMVRFASVAQSAELVRARWPEALVTVLTDSPDVD
                     VLAAGRVQAWVVGPGIGTDAFAEKLVAQVLSTNLPVVVDADALTIVARNPALVATRGA
                     PTVLTPHAGELTRLLGLEQEARRDVEARRLQHARAAAQQLRATVLLKGSTTVVCGPDG
                     VTRVNSNGTPWLATAGSGDVLSGITGALFAGGLSAFDAASCAAYLHGAAGR"
     gene            complement(1388..>2024)
                     /locus_tag="VGD55_12205"
     CDS             complement(1388..>2024)
                     /locus_tag="VGD55_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0871155.1"
                     /translation="KTFLTQLVAVYLVALYLSQVRATQYGDDVATVVHELAEMPRNVA
                     QVLTTMEPVREIARSLAGVTTVLFLGRHVGYPIALEGALKLKELAYMHAEGFAAGELK
                     HGPIALIEEGVPVVVVVPSPLGRSVLHAKIVANIQEVRARGARTIVIAEEGDDSVVPY
                     ADELIRVPAVPTLLQPLVSVIPLQVFACELAVARGNNVDRPRNLAKSVTVE"
BASE COUNT          355 a          727 c          646 g          296 t
ORIGIN      
        1 cgccctgccg cgccgtggag ataagccgcg cacgatgcgg cgtcgaacgc ggaaagtcca
       61 ccagcgaaca gcgcccccgt gatgcccgag agcacgtcgc cgctgcccgc tgtcgcaagc
      121 catggcgtgc cgttggagtt cacccgagtc acgccatcag gcccgcaaac caccgtcgtc
      181 gaacccttca acaacaccgt cgcccgcagt tgctgcgcag cggcgcgagc atgctgaaga
      241 cgccgggcct cgacgtcgcg ccgtgcctct tgctcgaggc caagcagccg ggtgagctca
      301 cctgcgtgcg gggtcaacac ggtcggggcg ccgcgggtcg ccaccagcgc agggttccgc
      361 gcgacgatcg tgagcgcgtc agcgtcgaca accaccggca ggttcgtgct cagcacctgc
      421 gccaccagct tctcggcgaa cgcatcggtg ccgataccag gccccacgac ccacgcctgc
      481 acgcgaccgg cggccagcac atcgacgtcc ggactgtcgg tcaaaaccgt caccagcgcc
      541 tcgggccagc gagccctgac gagctcggcc gattgcgcaa cggaggcgaa tcgcaccatg
      601 cccgcgcccg cccgcagcgc gccaccggtg gcgagcagcg ccgcacctgt gtacgtcgcg
      661 ctgccggcga cgatgccgac gacaccgcgg ctgtatttat cggattcagc ctcggccgcc
      721 ggcagcaacg tctcgacgtc gtgagcttgc agcgcggtga tcgaggcggt cggcagcatc
      781 ggcccgagac cgatgtcgac gagctcaacc gcgccggcgt gctcggcgcc cgggtcgatc
      841 agcaggcctg gcttccaggt accgaacgtc accgtgacat cggcgcggat tgcacgacct
      901 tcgacctcgc cggtgtcggc atcgacgccg ctcggtacgt caaccgcgac gaccaacgcg
      961 tcggcgggaa tcaactcgac cacggcggcg gcgtcatcac gcaagccgcc cttgccgccg
     1021 atgcccacga tgccgtcgat gacgagttcg gcattggcca tcgcgtcagc gccaacgatg
     1081 cggccgcctg cggtgcgcaa agcggtgagc ccaccggcgt gggccatcga gccgagaagc
     1141 accgccgaca ccactgcacc gcgctgcgca agcaacgcgc ctgcgaacaa tgcgtccccg
     1201 ccgttgtcac ctgagccgac gagcaacacg acgcgcgagc cgtaaacctt cggcagcagg
     1261 cgcgcgcatg ttgcggccag accggtagcc gcccgatcca tcaacgtgcc ggcgggtagc
     1321 cacgacatca gcgccgcttc ggccgcgcgg acatttgcta cgtcgtgggc gtgcctcatt
     1381 gccgttgtta ttcgaccgtg acggacttcg cgagattgcg cggccggtcg acgttgttgc
     1441 cgcgcgccac agccagctcg caggcgaaga cctgcagcgg aatcaccgac accaacggct
     1501 gcaacaacgt cggtaccgcg ggcacccgga tcagctcgtc ggcgtaaggc acgactgagt
     1561 catcgccttc ttcggcgatc acgatcgtgc gggctccgcg agcccggact tcctggatgt
     1621 tcgccacgat tttcgcgtgc agcaccgagc gtccgagcgg tgacggcacg accacgacca
     1681 ctggaacccc ctcttcgatg agggcgatcg gcccgtgctt cagctcgccg gctgcgaacc
     1741 cttcggcgtg catgtaggcc agctctttca acttcagcgc accttcaagg gcgatcggat
     1801 atccgacgtg acggccgaga aaaagcacag tcgtgacacc ggccagtgac cgcgcgatct
     1861 cgcgcaccgg ctccatcgtt gtgagcacct gcgcgacgtt tcgaggcatc tcggccagtt
     1921 cgtggacgac ggtcgcaacg tcgtcgccgt attgcgtggc acgcacctgg ctgaggtaga
     1981 gcgcgacgag gtagaccgca acgagttggg tgaggaacgt cttc
//
LOCUS       DASWVF010000693         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_197948, whole genome shotgun sequence.
ACCESSION   DASWVF010000693 DASWVF010000000
VERSION     DASWVF010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197948"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..997)
                     /locus_tag="VGD55_12210"
     CDS             complement(101..997)
                     /locus_tag="VGD55_12210"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0871156.1"
                     /translation="MTDYKHIASGKVRDLYEMDDGRLLFVASDRISAYDFVLPTPIPD
                     KGRVLTAMSIWWFDQLADVVPNHLLSTDVPPEFEGRATICERLDMLPVECIARGYLTG
                     SGLADYRRTGAVSGLPLPPGLVDGSRLPEPLFTPSTKAPIGEHDENISLGDVVDTVGA
                     EAAAELERLTLAVYGRAEEIARARGIILADTKFEFGRRRAGGAHDVNSAAESGDATIV
                     LADEVLTPDSSRFWPADQYEPGRAQPSYDKQYVRDWLTSPASGWDKSSEKPPPPLPDD
                     VVAKTREKYIEAYERLTGLTWS"
     gene            complement(1102..>2241)
                     /gene="purB"
                     /locus_tag="VGD55_12215"
     CDS             complement(1102..>2241)
                     /gene="purB"
                     /locus_tag="VGD55_12215"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0871157.1"
                     /translation="HKGMTSRDLTENVEQLQVRESLIIVRDRMVAALARLARLATENA
                     ELVMVGRTHNVAAQATTLGKRFASAGEELLIALQRIENLLERYPLRGIKGPVGTAQDM
                     LDLVDGDAAKLAQLESSVARHLGFDRISTSVGQVYPRSLDYDVVTALVQAAAGPSSLA
                     TTIRLMAGLELVTEGFKAGQVGSSAMPHKMNTRSCERVNGLAVVLRGFASMTSELAGD
                     QWNEGDVSCSVVRRVALPGAFFAADGLFQTFLTVLDDFGAYPAVVQRELDRYLPFLAT
                     TKVLMAAVRRGVGREVAHEVIREHAVAVALALREKGSDRNDLLERLASDERLKLTSTD
                     LDALVSTPLSFTGAATAQVAAFCDQVALVTALHPDAAFYVPGEIL"
BASE COUNT          361 a          736 c          764 g          380 t
ORIGIN      
        1 ctcggcgccc ataaagaccc tgggcaccca cgaggtcgtg gtgcgcgtga ctggagatgt
       61 ctccgccaag gtcgcgatcg aggtcgtctc cgcctaacgg ctacgaccag gtcaggccgg
      121 tcagccgctc gtaggcctcg atgtacttct cgcgggtctt cgccacgacg tcatcgggca
      181 gcgggggcgg cggcttctcc gagctcttgt cccagccgga cgcgggcgag gtcagccagt
      241 cgcgcacgta ctgcttgtcg tacgacggtt gcgcgcggcc cggttcgtat tggtcggccg
      301 gccagaaccg cgacgagtcg ggggtgagta cttcgtcggc cagcacgatg gtcgcgtcgc
      361 ccgactcggc cgcgctgttc acatcgtgcg cgccgccggc gcgacgccgt ccgaattcga
      421 atttcgtgtc ggctaagatg atgccgcggg cccgcgcgat ctcttcggcg cgaccgtaga
      481 cagccagcgt cagccgttcg agttcggcgg ccgcttccgc gccgacggtg tcgacgacat
      541 cgccgagcga aatgttctcg tcgtgctcgc cgataggggc cttagtcgac ggcgtgaaca
      601 gtggctccgg cagccgcgat ccgtcgacga gccccggcgg caaagggagt cccgaaactg
      661 cgccggtacg ccggtaatcg gcgagccccg aaccggtgag gtagccgcgg gctatgcact
      721 cgaccggcag catgtcgagc cgctcgcaga tcgtcgcgcg gccctcgaac tcgggtggca
      781 cgtcggtcga cagcaagtgg ttggggacga cgtcggcgag ctggtcgaac caccagatcg
      841 acatggcggt caacacgcga cccttgtcgg ggatcggcgt gggcagcacg aagtcgtagg
      901 cggagatgcg gtcgctggcg acgaacagca accgcccgtc gtccatctcg tagaggtctc
      961 tgactttgcc tgaggcgatg tgcttgtagt cggtcacgcc ggtgagtttc tcagaaccgg
     1021 ggcggacgcc gaccagcggc ctccatctcg actgtcagac agtacgggtc tagagggcag
     1081 tgcgcgtctg tcagcgggca gctacagaat ctcgcccggg acgtagaacg cggcgtccgg
     1141 atgcagggca gttaccagcg cgacctggtc gcagaacgcc gccacctgcg cggtcgcggc
     1201 gccggtgaag ctgagcggtg tcgacacgag ggcgtcgagg tccgtactcg tgagcttcag
     1261 gcgctcgtca ctcgcaagcc gttcgagcag gtcgttgcgg tcgctgccct tctcgcgcaa
     1321 agccagtgcg accgcgaccg cgtgctccct gatcacctcg tgcgcgactt cgcggccgac
     1381 tcccctgcgg accgccgcca tgagcacctt cgtcgtcgca aggaacggca ggtaacggtc
     1441 gagctcgcgc tgcacgacgg cggggtaggc accgaagtcg tcgagaaccg tgaggaaggt
     1501 ctggaacaga ccgtcggctg caaagaatgc gcccggcagc gcgactcgtc gcacgaccga
     1561 gcacgacacg tcgccctcgt tccactggtc gcccgcaagc tcactggtca tcgacgcgaa
     1621 gccgcgtaag acgaccgcca gcccgttgac gcgttcgcac gaccgggtat tcatcttgtg
     1681 cggcatggca ctcgagccga cctgcccagc cttgaatcct tcggtcacca gttcgaggcc
     1741 ggccatcaga cggatcgtcg tcgcgaggct cgacggtccg gcggccgctt gtacgagcgc
     1801 ggtgaccacg tcgtagtcga gcgagcgcgg gtacacctgg ccaacgctcg tgctgatccg
     1861 atcgaacccc agatggcgtg ccacgctgct ctcgagttgc gcgagtttgg cggcgtcgcc
     1921 atcgactaga tcgagcatgt cttgtgcggt gccgaccggg cccttgatgc cacgcaacgg
     1981 gtagcgctcg agcaggtttt cgatgcgctg cagggcgatg agcaactctt cgccggccga
     2041 cgcgaaacgc ttgccgagcg tggtcgcctg cgccgcgacg ttgtgcgtgc gcccgaccat
     2101 gacgagctcg gcattttcgg tggcgagccg ggcgagccgg gcgagcgcag ccaccatgcg
     2161 gtcgcgcacg atgatcagtg attcgcgcac ctgcagctgc tcgacatttt cggtcaggtc
     2221 acgcgacgtc atgcccttgt g
//
LOCUS       DASWVF010000694         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_95913, whole genome shotgun sequence.
ACCESSION   DASWVF010000694 DASWVF010000000
VERSION     DASWVF010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95913"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGD55_12220"
     CDS             <1..598
                     /locus_tag="VGD55_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871158.1"
                     /translation="TSSTRRQAERFMCRHATQPLPAEPHSAATARGFLRRRFAEWELV
                     PLLDDAEVALTELVTNALRHAHTPLEVSLSSENAMVEIAVCDGSPVFPTLRPERKDLD
                     SEIAEVLAEEQTLGAVLPDDDPRLAIGGAGPLTGGRGLLIVQAIAAQWGASPLRDGKA
                     VWIRTPVPDGWPHSAGCPCATSPQATRLASGHRVVHRN"
     gene            709..1938
                     /locus_tag="VGD55_12225"
     CDS             709..1938
                     /locus_tag="VGD55_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY0871159.1"
                     /translation="MTRDGDLSAEDQLTNFRAITDSKLGRLDVEDLLVELLERVRGIL
                     DADTAAVLLLEPETGDLVARAARGIEEEVRQGVRVPMGVGFAGRIADSKEPVLLDRVD
                     STTVANPILWEKGIRTMLGVPLLVGDEVLGVLHVGRLEHRPFNSSDAELLQVVADRVA
                     GAVQARQLAVERAATALLERSLLPGRLPDCAGLEFAARYITPEEPVGGDWYDVFTVPS
                     GDLWVVTGDVAGHGLHAAVVMGRVRSALRSYALLSSDPAQVLELTSRKVQHFEAETMV
                     TAVCATASFPYREFRVATAGHPPPLVVPADRPGFYPDLPVGPPLGVHAVAGRSSAVVG
                     LAEGSVLLFYTDGLIERRGESIDEGLERLRCAFSADSPEAVCRQLAKAMLDDGIQDDV
                     AIVAVRRPELPSVWPLA"
BASE COUNT          343 a          703 c          719 g          383 t
ORIGIN      
        1 aacatcctcg accagacgcc aagcggagcg attcatgtgc cgacacgcga cgcagccgtt
       61 gccagccgag ccccactcag ccgcaaccgc ccgcggattt ttgcgtcgtc gcttcgccga
      121 gtgggagttg gttccgcttc ttgacgacgc cgaagtcgct ctcaccgagt tggtgaccaa
      181 cgcgttgcgt cacgcgcaca cgccactgga ggtcagcctg agctccgaga acgcgatggt
      241 ggagatcgcg gtctgcgacg ggagcccggt ctttcccacg ctgcgtcctg agcgtaaaga
      301 cctcgattct gagattgcgg aggtgctcgc tgaggagcaa accctcggag cggtgctacc
      361 cgacgacgac ccccgtctgg ccatcggcgg cgcaggaccg ctcacgggcg gccgaggact
      421 gctcatcgtc caagcaatcg ccgcccagtg gggggcaagc ccgcttcgcg acggaaaagc
      481 cgtgtggatc cgaacaccag tccccgacgg ctggccgcat tcggccggct gcccctgcgc
      541 aacctcgccc caagcgacac ggctggcctc cggccaccgg gttgtccacc gaaactgacg
      601 atggcgttga catgaggact gggtcgggac gggaatcacc atgccgttgc cttggctgtt
      661 gccgtcgcgt ttgccgtcgg ctcgagctaa cacggatacg ctctgcccgt gacgagggac
      721 ggcgacctca gtgccgaaga ccagctgacc aactttcggg ccatcactga cagcaagctg
      781 ggccgtttgg acgtcgagga cctgctcgtc gagttgctgg agcgtgtgcg cggcatcctc
      841 gacgcggata ccgccgccgt gttgctcctc gagcctgaga ctggcgacct cgtcgcgcgg
      901 gcggcgcgcg gcatcgagga agaggtccgc cagggcgttc gggtgcctat gggggtggga
      961 tttgccggtc gcatcgccga ttcgaaggag ccggtactcc tggaccgcgt ggactcgacg
     1021 acggtcgcga acccgatcct gtgggagaag gggatccgga ccatgctcgg cgtccccctc
     1081 ttggtaggcg acgaagtcct gggcgtgctc catgtagggc gcctcgaaca ccgacctttc
     1141 aactcgtccg acgcggaact gctgcaagtg gtcgccgacc gggtcgcagg ggcggtccag
     1201 gcccgtcagt tggcggtcga acgagcggca accgcgctct tggagcgaag cctcttacct
     1261 ggtcggttac ccgactgtgc cggtctggag ttcgccgccc gctacatcac ccccgaagaa
     1321 cccgtcggcg gtgattggta cgacgtgttc accgttccct ccggtgatct ttgggtagtg
     1381 acgggcgacg tcgccgggca tggtctgcat gcagcggtgg tgatgggccg agtgcgaagt
     1441 gccttgcgtt cctatgctct gctgagcagc gaccccgccc aggtattgga gctcacgagc
     1501 cgcaaggtcc agcacttcga ggcggaaacg atggtgaccg cggtgtgcgc gaccgcctca
     1561 ttcccgtacc gcgagttccg ggtcgcgacg gctggccacc cgcccccgct tgtggttccc
     1621 gccgaccggc cgggctttta cccggacttg cctgttggtc caccgctggg ggtgcatgcc
     1681 gttgccggtc gttcgtcggc cgtggtgggg ctcgccgaag gatcggtgct gctcttctat
     1741 accgatggcc tgatcgaacg caggggcgag agtatcgacg aaggtctgga aaggctgcgc
     1801 tgcgcattca gcgctgatag cccggaggcg gtgtgtcgcc agctcgccaa agcgatgctg
     1861 gacgacggga ttcaagacga tgtcgccatc gtcgccgtcc ggcgacccga acttccctcc
     1921 gtgtggccac tggcttgagc cgaccgcctc ggaagcggcg cccctcaccg tcagtcgcgt
     1981 gcctacttga cgctgaactc cgtcagcacg caacgccata aaggcgtggc cacgggttag
     2041 gccgttctgg gcgactgact tgccagccgc agcctgacgc tcggcggtga cctgcgggca
     2101 gggctctaca ccggcttttc aacgtagtcg ccagccgggc agaaggta
//
LOCUS       DASWVF010000695         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_121442, whole genome shotgun sequence.
ACCESSION   DASWVF010000695 DASWVF010000000
VERSION     DASWVF010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121442"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..807)
                     /locus_tag="VGD55_12230"
     CDS             complement(166..807)
                     /locus_tag="VGD55_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0871160.1"
                     /translation="MTSSATPTKTHPYHHGDLPHALIEAAVELAREGGPGAIVLREAA
                     RRVGVSPNAAYRHFDSLPDLVVQVALVALHRMALTMQAELERCVSTDDPGLDAQVRME
                     AVGRGYVRFALEEPGLFATAFTSPEKTGMHERANADNLLTPETLLTDRLDELVATGVL
                     NPDEYDAAAKTGWGIAHGLSMLLLGPLSGVPLDERQALIDACLHIVTRGIRSS"
     gene            1002..>2284
                     /locus_tag="VGD55_12235"
     CDS             1002..>2284
                     /locus_tag="VGD55_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0871161.1"
                     /translation="MSAGFDNPASASSKAKVLLDQKFGGDPDMIFLVHATSGTVDGSA
                     ATSAGEALAAKLKADPRLTGVTSYWTTHAPSLRSKDGTDALILTRVVGDDNKAATTST
                     KLLSDYAHENTPAATVRIGGALGTGIGGQVTKDLAVAESIAVPLTLILLMVVFGSVVA
                     ALLPLSIAVLAIFGTLAELNVLTHVTSVSIFAINLTTALGLGLGIDYALLMVSRFREE
                     LANGVDVAEAVARTTATAGRTIAFSALTVAAALAAMIVFPVYFLKSFAYAGVGVTAFA
                     AMGALIVMPALLTVLGHRVNAGRIPGIKAVRSAETPFWGRLAKGVMRRPIIAAVPVIA
                     VLLFMATPLLKVTFAAPDDRALPTSAASHQVGDALRNDFATAPSVTDVVVSPPLSPAT
                     LATYEAALSAVPGVQHVADSGTVSGTQRLTITTGL"
BASE COUNT          394 a          758 c          725 g          407 t
ORIGIN      
        1 gccgaactgt tgccggagtt gatcagccgc gacgaatggg gattcccact gattcccgat
       61 tcgaccgtgc cagcaccact gcgcgatcac gccgagcggg caatcgcggc gtgtccaagg
      121 ttggcgttgc ggctctcaaa agcctagagc gtcatggaaa aaggcctaac tcgaccgaat
      181 ccctcgggtg acgatgtgca gacaggcatc gataagtgcc tgccgctcgt ccaacggaac
      241 gcccgacagc ggaccgagta gcagcatcga caggccgtgc gcgatacccc aaccggtctt
      301 ggccgcggcg tcgtactcgt cggggttgag cactccggtg gcgaccagtt cgtcgagccg
      361 atcggtgagc agcgtctccg gcgtcaacag gttgtcagca ttggcgcgct cgtgcatgcc
      421 cgtcttctcc ggtgaggtga acgcggtcgc gaatagtccg ggttcctcaa gcgcgaatcg
      481 gacgtagccc cgacccaccg cctccatccg cacctgcgcg tcaagaccag ggtcgtcggt
      541 cgacacgcat cgctcgagct cagcctgcat cgtcaacgcc atccggtgca gcgcaaccag
      601 tgcgacctgc acgaccagat cggggagcga gtcgaagtgc cggtaagccg cgttgggcga
      661 cacgccaacc cgccgggctg cctcccgaag gacgatcgcg cccggcccac cttcgcgagc
      721 cagttcgacg gccgcctcga tgagtgcatg gggcagatcg ccgtggtggt aggggtgggt
      781 tttggttggt gtcgctgaac tagtcacgcg aaacatgttg acagcgtgaa catccagatg
      841 gcaagttcac ggtgtcaaca tcgtgcagcg aagtcaggga gtctgtcgtg ttctacacac
      901 tgtgaacggt catcgtcagg cgcgcccggc tggttctggc gctcagtatc gcggcgctgc
      961 tggtcttcgc cgttctcggc gtcggtgtct tcggccgtct gttgtcggcc ggcttcgaca
     1021 acccggcgtc cgcctcgagc aaggccaagg tgttgctcga tcagaagttc ggcggcgatc
     1081 cggacatgat cttcctggtg cacgcgacca gcggcacggt cgacggctcc gcggcgacga
     1141 gcgccggcga ggccctcgcg gccaaactca aagccgaccc gaggttgacg ggcgtcacct
     1201 cgtactggac gactcacgca ccgagcctgc gttcgaagga cggcaccgac gcgctgatcc
     1261 tgacccgggt agtcggcgac gacaacaaag ccgccaccac gtcgaccaaa ctgctctccg
     1321 actacgcaca tgagaacacc ccggccgcaa ccgtgcggat cggcggcgca ctaggcaccg
     1381 gcatcggcgg ccaggtcacc aaagatctcg ctgtcgccga gagcatcgcg gtgccactca
     1441 ctctcatctt gctgatggtc gtattcggca gcgtggtcgc tgcactgctg ccactgtcca
     1501 tcgcggttct cgcaatcttc ggaacgctcg cagaactcaa cgtgctcacc cacgtcacga
     1561 gcgtcagcat cttcgccatc aacctgacca ccgcgctcgg cctcggtctg ggcatcgact
     1621 acgcgttact gatggtgagc cggttccgtg aagagctcgc gaacggcgtc gacgtagcag
     1681 aagcagtggc gcggacgacg gcaactgccg gtcgcacgat cgctttctct gcgttgacgg
     1741 tggccgcggc gttggccgcg atgatcgtgt tccctgtcta cttcctgaag tcattcgcct
     1801 acgcaggcgt cggtgtgacc gcgttcgccg cgatgggcgc gttgatcgtg atgcccgcgt
     1861 tgctgaccgt gctaggtcac cgggtgaatg ccggccgcat tcccggaatc aaggcggtgc
     1921 gcagtgccga gaccccgttc tggggtcggc tggccaaggg cgtcatgcgg cgtccgatca
     1981 tcgctgcggt gcccgtcatc gccgtgctgc tcttcatggc gacgccgctg ttgaaggtga
     2041 ccttcgcagc gcccgacgac cgagcgctgc cgaccagcgc ggcgagccac caggtcggcg
     2101 acgcgttgcg caatgacttc gccacggcgc ccagtgtcac cgacgtcgta gtctccccac
     2161 cgctgtcacc ggcaactctg gcaacgtatg aagccgcact aagtgcggtg cctggcgtgc
     2221 agcacgtcgc cgactccggt acggtctcgg gcacccaacg gctcacgatc acgacgggcc
     2281 tgta
//
LOCUS       DASWVF010000696         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_248846, whole genome shotgun sequence.
ACCESSION   DASWVF010000696 DASWVF010000000
VERSION     DASWVF010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248846"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..>1566
                     /locus_tag="VGD55_12240"
     CDS             155..>1566
                     /locus_tag="VGD55_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY0871162.1"
                     /translation="MTITVTDGTGGGVMAGILSNATTRRQQEDALIAIATRYHASGID
                     LDYENMAFFANGNPSLVAPTRAGFDAMVQELSAMLHARGMILAVDVITKLSEPGGSPA
                     QQVYDYPTIGRYADRVRIMTYDEHAQGTGYPGGPVSGISWLEPIIAFATTVIPPAKLY
                     MGVPLYGYDWSSKTNRGSAVSYTQAANLMAQYHAMRQWSIPDGSPFFTYTDASGAHHT
                     VWYDDAESLQARLPLVGKYGLGGIAMWSFGNEDPAIWGVLRAATYGPNPFGSVGSAAL
                     WPGGVRVTGWSIDPNSTAPINVDLYVDGKFVTRTLAGVDRPDVGNIYFAYGTTHGFDT
                     TVDLPAGQHQICAYGINVGTGTTNPKLGCIGATVPTNNPTGHLETASGKAGVLTVAGW
                     TLDPDTAAPLTVAVYVDGKLTGETTANVSRPDVAKAYPPWGDDHGFSTSVDVSGGTHQ
                     VCAYAINQRLGTTNPSLGCDT"
BASE COUNT          289 a          523 c          486 g          268 t
ORIGIN      
        1 caccgccgac gcgtcgctta acccagacct cttttccgac gtctccgagt tctggttcca
       61 cgcggtaagc gcgacgtcga tcgtggcgtc tggaaccacg cccgatgccc agctgacgtc
      121 gtcgatcgcg agcatccgca gccacgatgt acctgtgacg atcacggtca ccgatggcac
      181 cggcggcggc gtcatggccg gcatcttgtc gaacgccaca acgcggcggc agcaagagga
      241 cgcgctgatc gcgattgcga ctcgctacca cgcgagcggc atcgacctcg actacgagaa
      301 catggcattc ttcgcgaacg gcaacccgtc gttggtcgcg cccactcgcg caggcttcga
      361 cgcgatggtg caagaactct cagccatgtt gcacgcgcgc ggcatgatcc tcgcggtcga
      421 cgtcatcacc aaactcagcg agcccggcgg ctctccggcg cagcaggtct acgactaccc
      481 gacgatcggt cgatacgcag accgggtgcg gatcatgacc tacgacgagc acgcgcaggg
      541 caccggttat cccggtggac cggtcagtgg catcagctgg ctcgagccga tcatcgcgtt
      601 cgctacgacg gtgatcccgc cggcgaagtt gtacatgggc gttccgttgt atggctacga
      661 ctggtcgagc aagaccaaca gaggcagcgc ggtctcttat acgcaggccg caaatttgat
      721 ggcgcagtac cacgcgatgc gtcaatggtc gatccccgat ggttcaccgt tcttcacgta
      781 taccgacgca tcgggtgcgc atcacacggt ctggtatgac gacgcggaat ccctacaggc
      841 gcggctgcca ctggtcggca agtacggcct cggtggcatc gcgatgtggt cgttcggcaa
      901 tgaggaccca gcgatctggg gagttctgcg cgcagcgacg tacggcccga acccgttcgg
      961 cagtgtcggc agcgcagcac tgtggcccgg tggcgtgcgg gtgaccggct ggtcgatcga
     1021 cccgaactcg accgcgccga tcaacgtcga cctgtacgtc gacggcaagt tcgtcactcg
     1081 cacgttagcc ggtgtcgatc ggcccgacgt cggcaacatc tacttcgcct acggcaccac
     1141 gcacgggttt gacaccaccg tcgacttgcc cgcgggacag catcagatct gtgcgtacgg
     1201 catcaacgtg ggcaccggaa cgacaaaccc gaagctcggt tgtatcggtg ccaccgttcc
     1261 gaccaacaac ccgaccgggc atctcgagac tgcctcgggc aaggctggcg tgctcacggt
     1321 tgctggttgg acgctcgacc cagacaccgc cgcgccgctg accgttgccg tctatgtcga
     1381 cggcaagttg accggcgaga cgaccgcgaa cgtgtcgagg ccagacgtcg ccaaggccta
     1441 cccgccttgg ggcgatgacc acggcttctc cacctcggtc gacgtgtcgg gtggcacgca
     1501 tcaggtctgc gcctacgcga tcaaccagcg gctcggcacg acgaacccga gcctgggttg
     1561 cgacac
//
LOCUS       DASWVF010000697         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_478366, whole genome shotgun sequence.
ACCESSION   DASWVF010000697 DASWVF010000000
VERSION     DASWVF010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478366"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGD55_12245"
     CDS             complement(<1..302)
                     /locus_tag="VGD55_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871163.1"
                     /translation="MGLFKSRDDQAARLQSFVFADPTGVGDVGAAGGIDIAAVQRVDA
                     SFDPTLFLAGATSLFTDVRAALSAGSIDPIAGRLSPELTSMFGNQLQYAMTAGHQLT"
     gene            complement(315..1355)
                     /locus_tag="VGD55_12250"
     CDS             complement(315..1355)
                     /locus_tag="VGD55_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0871164.1"
                     /translation="MGLGHDISREFIAVPDGAKQQLLYKWPDRQIRRFTSAIVDVDEL
                     AIFISQGRVIGILQPGRHQVDATELPFLGDVADKLSGGNAYDSELFFVSTHEFPDLTF
                     GGPVDSVRDPETRLIVQLRAYGDFALQVVDPAALITKLTGTVDLADPAAVIGWAAEQV
                     LKAVRTVVVGHVVNDEWPVLGLAARTPDIEAGTVAAANQALTGYGIRVTRLGNVTVSL
                     SDEDAEQLRTLARDTAYTALTGSFGAYAQGEALLGAGKGMANGQGGGNEALGIASLGL
                     GAGVLGAFANQAAPAAVPAGAPVAAPAAAPAVTAPVATATPAAFCSHCGHALVGGALF
                     CSACGSAVATAP"
     gene            1476..>1941
                     /locus_tag="VGD55_12255"
     CDS             1476..>1941
                     /locus_tag="VGD55_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531116.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0871165.1"
                     /translation="MPVPGELLFVAPRRAKPPRHLADLTPADRRAAVGELGHPQFRAT
                     QLSTHYFSRLVDDPAAMTDVPAASREALVGALLPTLLTPVREVSCDDGATRKTVWRLF
                     DGTLVESVLMRYFGAISAGRTELGREADEPDATTRGRDRVTICVSSQAGCGMN"
BASE COUNT          313 a          642 c          678 g          308 t
ORIGIN      
        1 gtgagctggt gtccagccgt catcgcgtat tgcaactgat tgccgaacat cgaggtgagc
       61 tcgggcgaca gccggccagc gatcgggtcg atacttcctg cactgagcgc ggcccgcaca
      121 tcggtgaaca gagacgtcgc acccgcgagg aacaacgtcg ggtcgaatga tgcgtcgacc
      181 cgctgcaccg ctgcgatgtc gatcccgcct gctgcgccga cgtcgccgac tccggtggga
      241 tcggcgaaga cgaacgactg gagtcgggcg gcctggtcgt cacgcgactt gaacagaccc
      301 atgtggtcag cagctcacgg tgcggtagcg acggcgcttc cgcacgcgga gcagaacagc
      361 gccccgccga cgagtgcgtg tccgcagtgg gaacagaaag cagccggagt ggcggtggcg
      421 accggcgcag tgacggcagg tgccgctgcc ggggctgcga ccggggcgcc agcaggtaca
      481 gccgcgggtg ccgcctgatt ggcgaaggcg ccgagaacgc ccgcaccgag gccgagtgac
      541 gcgatgccga gggcttcgtt gccgccgccc tggccattgg ccatgccctt gccggcgccg
      601 agcagcgcct cgccttgtgc gtacgcgccg aacgagccgg tcaaagccgt gtaggccgtg
      661 tcgcgggcaa gcgtgcgcag ctgttcggcg tcctcgtcag agaggctcac cgtcacgttg
      721 ccgagcctgg tgacccggat tccgtagccc gtgagtgcct gattcgcggc ggctacggtg
      781 cccgcctcga tgtcgggagt gcgggccgcg aggccgagca ccggccactc gtcgttcacg
      841 acgtgaccga cgaccacggt gcgaaccgcc ttcagaacct gctctgcagc ccagccaatc
      901 acggcggccg ggtcggccag gtcgaccgtc ccggtcagct tcgtgatgag cgcagccggg
      961 tcgacaacct gaagcgcgaa gtcgccgtag gcccgcagct gcacgatgag gcgggtctcg
     1021 ggatcgcgca ccgagtcgac cggaccaccg aacgtcaagt cggggaactc atgcgtgctg
     1081 acgaagaaaa gttcggagtc gtaggcgttt ccaccgctga gcttgtcggc gacatcgccg
     1141 aggaagggca actcagtcgc gtcgacctgg tgtcgaccgg gttgcaggat gccgatgacc
     1201 cggccctgcg aaataaagat cgccagctca tcgacgtcca cgatcgcgct cgtgaaacgg
     1261 cggatctggc ggtccggcca cttgtacagc agttgctgct tggcgccgtc tgggaccgcg
     1321 atgaactcgc gactgatgtc gtggccgagg cccatcgcgt ctcctgggat cgagtgcgcg
     1381 ggtcgaggcc cgctcctcct gcttgtcggt ggcttgggag ccctccttga gcagtgagca
     1441 cgcggcgctc ccgggactga gagactggac gaaccatgcc tgtccccgga gaactgcttt
     1501 tcgtcgcccc gcgccgagcc aagccgcctc gccatctggc cgatctgacg cctgccgaca
     1561 ggcgtgcggc cgtcggcgag ctcgggcacc cccagttccg ggcgacccag ctatcgacgc
     1621 attacttcag caggctcgtt gacgaccctg cggcgatgac cgacgtcccg gccgcgtcgc
     1681 gcgaagcgct tgtgggcgcc ttgctgccga ccctcctgac gcccgttcgc gaggtttcgt
     1741 gcgacgacgg cgcgacccgc aagaccgtct ggcggctgtt cgacggaacg ctggtcgagt
     1801 cggttctgat gcgctatttc ggtgccatct cggctgggcg gaccgagctc ggtcgtgaag
     1861 cagacgagcc tgacgcgacg acccgagggc gcgaccgggt gacgatctgc gtgtcgagcc
     1921 aggccggctg cgggatgaac t
//
LOCUS       DASWVF010000698         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_95958, whole genome shotgun sequence.
ACCESSION   DASWVF010000698 DASWVF010000000
VERSION     DASWVF010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95958"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VGD55_12260"
     CDS             complement(<1..810)
                     /locus_tag="VGD55_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY0871166.1"
                     /translation="MADPKGFLTTERETPQTRPVDVRIMDWKEVYYPFPHADLEKQAG
                     RCMDCGIPFCHNGCPLGNLIPEWNDLVWRRDWREASERLHATNNFPEVTGRLCPAPCE
                     SACVLAIDPSQTGGSVTIKQVEHEIADRAWDDGWVTPQVPGRQTGKTVAIVGSGPAGL
                     AAAQQLTRAGHTVAVYERADRIGGLLRYGIPEFKMEKAVLDRRLAQMSAEGTRFRPGI
                     EIGVDMTGRELRSRYDAVVLAIGATSPRDLPITGRHLGGVHQAMEYLPIANR"
     gene            complement(803..5326)
                     /gene="gltB"
                     /locus_tag="VGD55_12265"
     CDS             complement(803..5326)
                     /gene="gltB"
                     /locus_tag="VGD55_12265"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY0871167.1"
                     /translation="MSSPAFGLYDKAHGLYDPANEHDACGVAFVADLAGGRSHDVVAN
                     ALTALRNLEHRGATGREIDTGDGAGLLTQIPDEFFRAVVDFTLPPAGGYAVGIAFLPT
                     DDVGAAAAVTAIERIVDDEGLHALGWRELPVVRDLAGPSARAMMPSFRQLFIAPNHAS
                     ITGLALERLTFRARKRIEREAEIYFGSLSPRTIVYKGMLTAPQLEPFFPDLSDQRFAS
                     ALALVHSRFSTNTFPSWPLAHPYRYIAHNGEINTIKGNRNWMRARESLLASSLIPGDL
                     EQLFPICTPGGSDSMSFDEVLELLHLGGRSLPHAILMMIPEAWEKNTEMDAERRAFYE
                     FHASVMEPWDGPACVTFTDGTLIGAVLDRNGLRPARYWVTDTGLVVLASEVGVLDIDP
                     ASIVRKGRLQPGRMFLVDTAAGRIVDDAEIKAQLAVEQPYDDWLHAGLLKLPQLPDRE
                     HIIYSHESVLRRQQTFGYTEEELRILLAPMARAGAEPIGSMGNDSPLAALSVRPRLLF
                     DYFSQLFAQVTNPPLDAIREELVTSLSGAIGPEQNLLLPGPISCRQIVVPFPVIDNDE
                     LAKILHVNDDGDLPGLAPFVARGLYHVDGGGEALERRLTNICAEISDAIEAGARIIVL
                     SDRDSDRERAPIPSLLLTAAVHHHLIREKARTKVGLIVEAGDIREVHHVALLVGYGAA
                     AVNPYLAMETVEDMARTGAITGMSGDVAVHNLVKALGKGVLKVMSKMGISTVASYTGA
                     QVFEALGLSHDLVDRYFTGTSSKLGGVGLDVIAGEVASRHTHAYPLGGVQQSHRRLQV
                     GGDYQWRREGEPHLFNPETVFRLQHSTRTGRYELFKDYTKLVDDQSRELMTLRGLFKL
                     REGVRPPVPLDEVEPVSSIVKRFSTGAMSYGSISSEAHETLAIAMNSLGARSNTGEGG
                     EDAERLYDNRRSAVKQVASGRFGVTSEYLTGADDLQIKMAQGAKPGEGGQLPGNKVYP
                     WIAKTRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKNSNPRARVHVKLVAEVGVGTV
                     AAGVAKAHADVVLISGHDGGTGAAPLTSLKHAGGPWELGLAETQQTLIANGLRERIVV
                     QTDGQLKTGRDVVIATLLGAEEFGFATAPLVVSGCVLMRVCHLDTCPVGVATQNPELR
                     KRFSGKPEFVVNFFEFIATEVREYLSALGFRTIDEAVGQVELLDTADAIEHWKASGLD
                     LAPMLHAPVEVDEPRRHLIDQDHGLEKALDNALIALAEGALNERIPVKFEIPIRNVNR
                     TVGTMLGHEVTKRYRGEGLPDNTIDITFTGSAGQSFGAFVPRGITLRLVGDANDYVGK
                     GLSGGRIIVRPYDEASFAAHHNVIAGNVLLYGATSGEMFVRGVVGERFCVRNSGANAV
                     VEGVGDHGCEYMTGGVVVVLGSTGRNFAAGMSGGIAYVVDLDPARVNTEMVEVEPLDA
                     ADCDQLLLLVRKHADETGSLLATEMLSQWSKTVERFAKIMPRDYKRVLAARARAEHEG
                     LDVNEQIMAASHG"
BASE COUNT          938 a         1752 c         1758 g          941 t
ORIGIN      
        1 gcggttggcg atcggcaggt actccatcgc ctgatgaacg ccgccgaggt gccggccagt
       61 gatcggcaga tcgcgtggcg acgttgcgcc gatggcgagt acgacggcgt cgtagcgcga
      121 gcgcaactca cgacccgtca tgtcgacgcc gatctcgatg ccagggcgaa atctggtgcc
      181 ctcggccgac atctgcgcaa gccgccgatc gagaaccgcc ttctccatct tgaactcggg
      241 aattccgtac cgcagcaagc cgccgatccg gtcggcccgc tcgtagaccg cgacggtgtg
      301 gcctgctcga gtcaactgct gcgccgcagc gagtccggcc gggcccgacc cgacgattgc
      361 gacggtcttg ccggtctggc ggccaggcac ctgcggcgtc acccacccgt cgtcccatgc
      421 cctgtcggcg atctcgtgtt cgacctgctt gatcgtgacc gagccgcccg tctgcgacgg
      481 gtcgatcgcg agcacgcacg ccgattcgca tggcgcggga cacagccggc ccgtgacctc
      541 ggggaagttg ttggtcgcgt gcaatctctc cgaggcctca cgccaatcgc ggcgccacac
      601 gaggtcgttc cattcgggaa tgaggttccc gagcgggcac ccgttgtgac agaacgggat
      661 accgcaatcc atgcagcggc cggcctgctt ttccaggtcc gcatgaggaa atgggtagta
      721 gacctctttc caatccatga tgcggacgtc gacgggccgc gtctgcggtg tctctcgctc
      781 ggtggtgaga aagcccttcg ggtcagccat gtgacgcagc catgatctgc tcgttgacgt
      841 cgaggccctc gtgctcggct cgtgcgcgcg cagcgagcac gcgcttgtag tcgcgcggca
      901 tgatcttggc gaacctctcg accgtcttcg accactgcga aagcatctcg gttgccagca
      961 acgagcccgt ctcgtcggca tgcttgcgca ccagcaacaa caactggtcg cagtcggcag
     1021 catccaacgg ttcgacctcg accatctcgg tgttcacccg tgcagggtcg aggtcgacga
     1081 cgtaggcgat gccgcccgac atgcccgcgg cgaagttacg cccggttgag ccgaggacga
     1141 ccacgactcc gccggtcatg tactcgcagc cgtgatcgcc aactccttcg acaacggcgt
     1201 tcgccccaga gttgcggaca cagaatcgct cgcccacgac accacgcacg aacatctccc
     1261 ccgatgtcgc gccgtagagc agcacgttgc cggcgatgac gttgtgatgg gcagcgaacg
     1321 acgcctcgtc gtaagggcgc acgatgatgc gaccgcccga caatcccttg cctacatagt
     1381 cattcgcgtc gccgacgagt cgcagcgtga tgccacgagg tacgaaggca ccgaacgact
     1441 ggcccgccga accggtgaat gtgatgtcga tcgtgttgtc gggaagcccc tcaccgcggt
     1501 agcgcttcgt tacttcgtgg ccgagcatgg tgccgaccgt acggttgacg ttgcggatcg
     1561 gaatctcgaa cttcaccgga atgcgctcgt tcaacgcgcc ctcggccaac gcgatcaacg
     1621 cgttgtcgag cgccttctcg agaccgtggt cttggtcgat gagatggcgg cgtggttcgt
     1681 cgacctcgac cggcgcgtgc agcatcggtg cgaggtcgag cccgctggcc ttccagtgtt
     1741 cgatcgcgtc ggcagtgtcg agaagctcga cctgaccgac agcttcgtcg atggtgcgaa
     1801 aacccagcgc agacagatat tcgcgcactt cggtcgcgat gaactcgaag aagttcacga
     1861 cgaactcggg cttgccgctg aaccgcttgc gcagttcggg gttctgcgtc gcgacgccga
     1921 ccggacatgt gtcgagatgg cacacgcgca tcagcacgca gcccgagacg acgagtggcg
     1981 cagtggcaaa accgaactct tcggcaccga gcaacgtcgc gatgacgaca tcacggccag
     2041 tcttcagctg cccatcagtc tggacgacga tgcgctcacg caacccgttg gcgatcagcg
     2101 tctgctgggt ttctgcgagg cccaactccc acgggccgcc ggcgtgtttg agcgacgtca
     2161 gcggggctgc gccggtgcca ccgtcgtggc cggaaatcag gacgacgtcg gcgtgcgcct
     2221 tcgccacgcc ggccgccacc gttccgacac cgacctcagc gacgagcttc acgtgaaccc
     2281 gcgcgcgggg gttcgagttc ttcagatcgt ggatcagttg cttgagatcc tcgatcgagt
     2341 agatgtcgtg gtgcggcggc ggcgagatca ggccgacacc gggggtcgag tgacgcgtct
     2401 tcgcgatcca cgggtacacc ttgttgccgg gaagctgacc gccctcaccc ggcttcgccc
     2461 cctgcgccat cttgatctgg aggtcgtcgg caccggtgag gtattcactg gtgacgccga
     2521 accggccact ggccacctgc ttcacggccg accgccggtt gtcatagagc cgctcggcgt
     2581 cttcgccacc ttcgccggtg ttcgacctgg caccgagtga gttcatcgcg atagcaaggg
     2641 tttcgtgggc ctcgctgctg atcgaaccgt agctcatcgc acccgtcgag aagcgcttca
     2701 cgatcgagct gaccggctcg acttcgtcga gcggcaccgg agggcgcacg ccctcgcgca
     2761 gcttaaacaa gccgcgcaac gtcatcagct cgcgcgactg gtcgtcgaca agcttggtgt
     2821 agtccttgaa cagctcgtag cggccagtgc gcgtcgagtg ctggagccgg aacaccgtct
     2881 ccgggttgaa caggtgcggc tcgccctcac gccgccactg gtagtcgccg ccgacctgca
     2941 accggcggtg cgactgctgc acaccaccga gcgggtacgc gtgcgtgtgc cgcgacgcga
     3001 cctctccagc gatgacatcg agcccgactc ctccgagctt cgacgacgtc cccgtgaagt
     3061 aacggtcgac aaggtcgtgc gaaagtccga gcgcttcgaa gacctgcgcg ccggtgtagg
     3121 aggcgaccgt cgagatgccc atcttcgaca tcaccttgag cacgcccttg ccgagcgcct
     3181 tgacgaggtt gtgcaccgca acgtcgccac tcatgccggt gatcgcgccg gtgcgcgcca
     3241 tgtcttcgac ggtttccatg gcgaggtacg ggttcaccgc agccgcgcca tagccaacca
     3301 gcaacgcaac gtgatgcacc tcgcgaatgt cgccggcttc gacgatgagg ccaaccttcg
     3361 tgcgggcttt ctcgcgaatg aggtgatggt ggacggcggc cgtcagcaac agggacggaa
     3421 tcggtgcgcg ctcgcgatcg gagtcgcggt cggacaatac gatgatgcgt gcgccagctt
     3481 cgattgcgtc ggagatctcg gcgcagatgt tggtgagccg gcgctcgagc gcctctcccc
     3541 cgccatcgac atggtacaag ccgcgcgcca cgaacggcgc cagccctggg aggtcgccgt
     3601 cgtcgttcac gtgcaggatt tttgcgagct cgtcgttgtc gatgaccggg aacggcacca
     3661 cgatctgccg gcacgaaatg ggcccaggca gcagcaggtt ctgctccggc ccgatggcac
     3721 ccgacaacga cgtgacgagc tcttcgcgga tcgcgtcgag gggtgggttg gtcacctgcg
     3781 cgaacagctg gctgaagtaa tcgaagagca gccgaggccg caccgacaaa gcagcgagtg
     3841 ggctgtcgtt gcccatcgag ccgatcggct cggcaccggc acgtgccatc ggcgcgagca
     3901 ggatgcgcaa ctcttcttcg gtatagccga aggtttgctg gcgacgcagg acggattcgt
     3961 gcgaatagat gatgtgctcc cggtcgggca actgcggaag cttcagcagc ccggcgtgca
     4021 accagtcgtc gtagggctgc tcgactgcga gctgcgcctt gatctcggcg tcgtcgacga
     4081 tgcgaccggc agccgtgtcg acaaggaaca tccggcccgg ctggagccgg cccttgcgca
     4141 cgatcgacgc ggggtcgatg tcgagaactc cgacctcgct ggcaagaacg acgagcccgg
     4201 tgtcggtcac ccagtaacgc gcaggacgaa ggccgttgcg gtcgagcacc gccccgatga
     4261 gcgtgccgtc agtgaacgtc acgcacgcag ggccgtccca cggttccatg acactcgcgt
     4321 ggaattcgta gaacgcccgc cgctcggcgt ccatctcggt gttcttctcc caggcctcag
     4381 ggatcatcat caagatggcg tgcggaagtg accggccacc aagatgcagc agttcgagca
     4441 cctcgtcgaa gctcatcgag tcgctgccgc ccggcgtgca gatcgggaag agttgttcga
     4501 ggtcgccggg gatcaaactg cttgccagca ggctttcgcg agcccgcatc cagttgcggt
     4561 ttcccttgat ggtgttgatc tcgccgttgt gcgcgatgta gcgatacggg tgcgcaagcg
     4621 gccagctcgg gaacgtgttc gtcgagaacc gcgagtgcac gagtgcgagc gccgacgcga
     4681 atcgctggtc tgacaagtcg gggaagaacg gctcgagttg cggcgcagtg agcatgccct
     4741 tgtagacgat cgtgcgcggc gatagcgacc cgaagtagat ctccgcttcg cgctcgatcc
     4801 gcttgcgcgc ccggaacgtc aaccgctcga gagcaagacc ggtgatgctc gcgtggttcg
     4861 gcgcaataaa caactgacgg aagctcggca tcatggccct ggcgctcgga ccagcaagat
     4921 cgcggacgac tggtagctca cgccagccga gtgcgtgcag accctcgtcg tcgacgatgc
     4981 gctcgatcgc ggtgacagca gcggcggccc cgacgtcgtc ggtgggcagg aaggcgatgc
     5041 cgacggcgta cccgcccgca ggcggcagcg tgaagtcgac gaccgcgcgg aagaactcgt
     5101 cggggatctg ggtcagcagt ccggcgccgt cgccggtgtc gatctcgcga ccagtcgcgc
     5161 cgcggtgctc gaggttgcgc agcgcggtga gggcgttcgc gacgacgtca tgactgcgcc
     5221 caccggcaag gtcggcaacg aacgcgacac cgcacgcgtc gtgctcgttt gctgggtcgt
     5281 agagtccgtg ggctttgtcg tagagtccaa atgcgggact ggacatggct ctcccgtcgt
     5341 ctcctgccgc gtgtgcaggc ccgacgcata gcgccgtaag ccgtgcacg
//
LOCUS       DASWVF010000699         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_171964, whole genome shotgun sequence.
ACCESSION   DASWVF010000699 DASWVF010000000
VERSION     DASWVF010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171964"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGD55_12270"
     CDS             <1..205
                     /locus_tag="VGD55_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871168.1"
                     /translation="NEALISGAGSRRAQARGVVGGILAGVGGSAGIAALSSSDNRSTL
                     VVIMLLGMVIGVALVVAAFSQRS"
     gene            complement(243..425)
                     /locus_tag="VGD55_12275"
     CDS             complement(243..425)
                     /locus_tag="VGD55_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871169.1"
                     /translation="MSGHSYEVRGGQPVRVGAPPRNLPADLQQFIDQPPWTLEMDGRG
                     RPVVDDRGELTTRPAM"
     gene            complement(603..980)
                     /locus_tag="VGD55_12280"
     CDS             complement(603..980)
                     /locus_tag="VGD55_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871170.1"
                     /translation="MQSSSHCPDEVVGKVTGFRERADGLELDIRLADPDRLPSAGKVS
                     SLLADDMVPSISFGFRDASSKPHPFLRGVQQFVKAVVDEVSFVAKPSIPGTRVLSLRH
                     AGRGHSLDAEVALARMRLHRMRA"
     gene            complement(1196..1268)
                     /locus_tag="VGD55_12285"
     tRNA            complement(1196..1268)
                     /locus_tag="VGD55_12285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1233..1235),aa:Ala,seq:ggc)
     gene            complement(1342..1977)
                     /locus_tag="VGD55_12290"
     CDS             complement(1342..1977)
                     /locus_tag="VGD55_12290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0871171.1"
                     /translation="MGGRKVVVWRHGQTAWNAQRRFQGQLDIELDDVGRAQVARAAVL
                     LTGVSPSAIVSSDLVRARETAQALADLTGLPVATDKRLREIDVGSWSGLTFEEVFERF
                     PDEAAAWRDGGEGRRGGGESLVEVGQRGVAAVWDALVDVGGGGTLVVATHGAAGRAIV
                     ASMIGLAPPSWRALGSLANCCWSLMGEGEAGWRLFDHNAGALPQPVVGDDR"
     gene            complement(1978..2418)
                     /gene="rsfS"
                     /locus_tag="VGD55_12295"
     CDS             complement(1978..2418)
                     /gene="rsfS"
                     /locus_tag="VGD55_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0871172.1"
                     /translation="MTATPRAIELAVAAAEAAADKLAHNIQVFDVSDRLVITDVFVLA
                     SAPNDRQVKSIVDAVEDRLREMGAKPASREGQHEGRWVLLDYSDVVVHVQHTEEHTFY
                     GLERLWKDCPQVPLPASVTDGLRQMDAESFDSRGAAAAGRASVL"
     gene            complement(2478..>2682)
                     /locus_tag="VGD55_12300"
     CDS             complement(2478..>2682)
                     /locus_tag="VGD55_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871173.1"
                     /translation="LQGENTDDMPNATAPSVVVIFGQTSVQINQGDAVARALGLPISD
                     VKVSDQSFSVADIVVKLGADYKG"
BASE COUNT          457 a          916 c          861 g          448 t
ORIGIN      
        1 gaatgaggca ctgatatccg gagccgggag ccgtcgcgca caggcccggg gcgttgttgg
       61 cggaatccta gccggcgtcg gagggtctgc cgggatcgca gcactgtctt cgagcgacaa
      121 tcgcagcaca ctcgtggtga tcatgctcct aggcatggtt atcggtgtgg cgttggtggt
      181 tgcggcgttc agccaacgct catgactatg accaggggta gcaaggccta acttcgtcga
      241 tgtcacatcg ccggtcgtgt ggtgagttcg ccacgatcgt ccacgacggg ccggccacgc
      301 ccatccatct ccagcgtcca cggcggctgg tcgatgaact gctgcagatc cgccggcagg
      361 ttccgcggcg gtgcgccgac ccgcaccggt tgcccaccgc gcacctcgta tgagtgcccg
      421 ctcaagctgt gaaacacctc cggcggcgga gcgtcctgct ggtcatgcca cgcgttgtat
      481 cgctccaggg cggccgcgtc agcgatgcgt cgccgcgcag aggctgcaca ccgggttcgt
      541 gaaggctccg gcggtcatcg gggtctcctt cgtcggttcg ggtgagggtc gccggatcag
      601 tttcatgcgc gcatcctgtg aagtctcata cgcgcgagcg cgacctcggc gtcgagactg
      661 tggccgcggc ccgcatgacg tagcgacaac acacgcgtgc cggggatcga aggcttcgcg
      721 acgaacgaca cctcgtccac gaccgccttg acgaactgct gaaccccgcg caggaacggg
      781 tgcggcttcg acgacgcatc ccggaacccg aagctgatac tcggcaccat gtcgtcggcc
      841 agcagcgacg ataccttccc ggcgctcggc aggcggtcgg ggtccgccag gcgaatgtcg
      901 agctcgagac catcggcgcg ttcacgaaac ccggtgactt tcccgacgac ctcgtcgggg
      961 cagtgcgatg aggactgcac gcaggcagac gattcgaccc tgttccacgc agcgcaggcg
     1021 ggcatagccc aggccgtgga tgaggtcgcc cgccgcgaga agctcgactc gaaagcctag
     1081 ttcgctacct cgcggcattc ggtctgctca gcggtggtga caaagtgttg acacgcggac
     1141 gtcactagcg gcgagttaca acctgcgcag accgtttgac ctgcggtttc gtgtgtggag
     1201 cttaggggac tcgaacccct gaccccttgc ctgccaggca agtgctctac cagctgagct
     1261 aaagccccgt cgtgccctcg cgggcgtcgc gaagtctagc ggacggcgag aacgacccgg
     1321 cgtccgtcaa tcaacgaccc ctcagcggtc gtcgccgacc acgggctgcg gcagcgcgcc
     1381 ggcgttgtgg tcgaacagcc gccatcccgc ctcgccctcc cccatcagcg accagcagca
     1441 gttcgcgagt gatcccagcg ctcgccatga cggcggcgcg agaccgatca tcgacgcgac
     1501 gatcgcccgc cctgcggctc catgcgtcgc aacgaccagc gtgccgccgc cgccgacgtc
     1561 aacgagcgcg tcccaaacgg ccgcgacccc tcgttgaccg acctcgacga ggctctcgcc
     1621 accgccgcgg cgtccctccc cgccgtcccg ccatgctgcc gcctcgtcgg ggaaccgctc
     1681 gaacacctcc tcaaacgtca gccctgacca cgagccgacg tcgatctcgc gcagtcgctt
     1741 gtcggtcgcg accggcaggc ccgtgaggtc ggcgagcgcc tgcgcagttt cgcgggcacg
     1801 gacaagatca gacgaaacga tggcggacgg cgagacgccg gtgagcaaga cagccgctcg
     1861 cgcgacttga gctcggccaa catcatcgag ttcgatgtcg agctgtccct ggaaccggcg
     1921 ctgcgcgttc catgccgtct gaccatggcg ccagacgact actttgcgac cgcccactca
     1981 gagcactgaa gcgcgacccg cggcagcagc gccgcgggag tcgaagctct cggcatccat
     2041 ctgacgcagg ccgtcggtaa ccgacgcggg cagcggcacc tgtggacagt ctttccacag
     2101 gcgctcgagg ccgtagaagg tgtgctcctc ggtgtgctgc acgtggacga cgacatcgga
     2161 atagtcgagc agcacccacc ggccctcgtg ctgaccctcg cggctcgcgg gcttcgcacc
     2221 catctcgcgc agccggtctt cgacggcgtc gacgatcgac ttcacctgac ggtcgttcgg
     2281 cgctgacgcc agaacgaaca cgtcggtgat cacaagtcga tcactgacgt cgaagacctg
     2341 gatgttgtgg gccaatttgt cggccgcggc ttcggcggcg gcgacggcga gctcgatcgc
     2401 tcggggggtc gcggtcatac ctgggtgagc tcctgtcgaa aaggcggccg gaaaacggcc
     2461 gctacccctc tatggtctca tcccttgtag tcggccccga gttttacgac gatgtcggcc
     2521 acgctgaacg actgatcgct gactttcaca tcggagatcg gcaggccaag cgcccgcgcc
     2581 acggcgtccc cttgattgat ctgtaccgac gtctggccga agatcacgac gaccgatgga
     2641 gccgtcgcat tgggcatgtc gtcggtattt tcgccctgaa gg
//
LOCUS       DASWVF010000700         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_121461, whole genome shotgun sequence.
ACCESSION   DASWVF010000700 DASWVF010000000
VERSION     DASWVF010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121461"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGD55_12305"
     CDS             complement(<1..205)
                     /locus_tag="VGD55_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0871174.1"
                     /translation="MGFPAGLFAANCYVVAPAAGERCVVIDPGQDAVPALSEVLREHR
                     LRPAAVLLTHGHIDHMWSAYPVCG"
     gene            361..1188
                     /locus_tag="VGD55_12310"
     CDS             361..1188
                     /locus_tag="VGD55_12310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0871175.1"
                     /translation="MAGKDRKRQIARERYLKRMAAKAEARAKARRRTAMGLSAGAVIA
                     IVGIVLAITLPGKNKSAANLSSPVSTASASPSAAPSSPIPPIPTTASGCTSKLSLVAS
                     PVANFPILPAGVDPQLKTEPIVTIPAGAVPTKTQVKDLVVGTGTTVGPHDSVTVNYLG
                     VNYVDCSEFDSSWSRDSAATFSLDEVVPGFTKGIGGDSASGIAPMKVGGRREIIVPPV
                     DGYGTTGQGAVKPNEELVFVVDVLAASAPSASPSAAGSGAASTSPAASTSPSQAATS"
     gene            complement(1222..>3048)
                     /locus_tag="VGD55_12315"
     CDS             complement(1222..>3048)
                     /locus_tag="VGD55_12315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0871176.1"
                     /translation="MARDPRVLVLKLADRLHNMRTLGFLPEEKQERKARETLEIYAPL
                     AHRLGMNTLKWELEDLSFATLFPKRYDEVVRLVTDRAPSRDTYLAGVIEQVNTQLRTA
                     KVKASVTGRPKHYYSIYQKMIVRGRDLNDIYDLLGVRVLVDSVRDCYAALGIIHATWN
                     PVPGRFKDFIAMPKFNLYQSLHTTVIGPDGKPVELQIRTHAMHRRAEYGIAAHWKYKE
                     DSAGGVESPSTGNDMAWLRQLLDWQKEAADPGEFLESLRFDIQGAEVYVFTPKGDVVA
                     LPQGSGPVDFAYAVHTEVGHRCIGARVNGRLVPLDSTLDNGDAVEIFTSKAQNAGPSR
                     DWLAFVKSPRARTKIRQWFAKERREDAIEDGKQAIARAMRKQGLPLQRLLSDDALGNL
                     ARDMHYTDVSGLYAAVGENHVTAQTVVQRLMLARGGEEGAVEDLAETINPNAPGPRRP
                     LRSTGDPGVVVKGASDIWVKLARCCTPVPGDAINGFVTRGNGVSVHRLDCDNMVSLTE
                     QEPDRVVAVEWAPGAASVFLVVVQVEALDRSRLLADLTQVLSDQHVNILAASVTTTRD
                     RVAVNRFTFEMGDPKHLGHVLKAVKAVDGVYDAYRVTSTARA"
BASE COUNT          511 a         1006 c         1017 g          514 t
ORIGIN      
        1 cgccgcagac cgggtaggcg gaccacatgt ggtcgatgtg accgtgggtc agcaagaccg
       61 ctgccgggcg cagccggtgt tcgcgaagaa cctcagacaa cgcggggacg gcgtcctgcc
      121 cgggatcgat cacgacgcac cgttcgccgg cggcgggggc aacgacgtag caattcgccg
      181 cgaagaggcc agcggggaac cccacgacga gcaagatcgt cctctctcgc gcaaacctgt
      241 gagctcgctc ggacctagca gcgaccatgc tgcgacctgg atcgcgctgc ggggtgagcc
      301 taccggcccc tctagactgt cggcgctgtc atatcccgca cgatccccca aggaggccgc
      361 gtggccggca aggaccggaa gcgtcagatc gcccgcgagc ggtacctgaa acggatggca
      421 gcgaaagccg aagcccgagc caaggcccgc cgccgcacgg ccatggggct gtcggccggt
      481 gccgtgatcg ccatcgtggg catcgtgctt gccatcacgc tgccaggtaa gaacaagtcg
      541 gcggccaacc tctcgtcacc ggtcagcaca gcgtcagcat cgccgtcagc ggccccgtcg
      601 tcgccgatcc caccgatccc gaccacggcg agcggctgca cgagcaagct cagcctcgtc
      661 gcctcacctg ttgccaattt cccgatcctg cctgcgggag tcgatccgca gctcaagacc
      721 gagccgatag tgacaatccc agccggagct gttccgacga agacgcaggt caaggatctc
      781 gtcgtcggca cgggcacaac ggtcgggccg cacgacagcg tgacggtcaa ctacctcggc
      841 gtgaattacg tcgactgctc cgagttcgac tcgtcgtggt cgagagattc ggctgcgaca
      901 ttctcactcg acgaggtcgt gccgggcttc accaaaggca tcggcggcga ctccgccagt
      961 gggatcgccc cgatgaaggt cggcggccgt cgcgagatca tcgttccgcc ggttgacggc
     1021 tacggcacga ctggccaagg tgcggtcaag cccaacgaag aactggtctt cgtggtcgac
     1081 gtgctcgctg cgtcggcacc gagcgccagc ccttcggcgg ccggctcggg cgcggcatcg
     1141 acaagcccag ccgcttcaac gagccctagc caggcggcga cttcgtagcc cgtcgtcgtt
     1201 gcgactgccc gcgcccgttc gtcaggcgcg ggcagtgctc gtcacgcggt aggcgtcgta
     1261 cacgccgtcg actgccttga cggccttcag gacgtgcccg aggtgcttgg ggtcgcccat
     1321 ctcgaaggtg aaccggttca cggcaacacg gtcgcgagtc gtcgtgaccg aggccgcgag
     1381 gatgttcacg tgctggtccg acagcacctg ggtgaggtcg gcgagtagcc ggctgcggtc
     1441 gagcgcctcg acctgcacga cgacgaggaa caccgacgcc gcgccgggag cccattcgac
     1501 cgctacgacg cgatcaggct cttgctccgt gaggctcacc atgttgtcgc agtcgagccg
     1561 gtgcacagaa acgccgttgc ctcgcgtgac gaaaccgttg atggcgtcgc caggcaccgg
     1621 cgtgcagcag cgcgccagct tgacccagat gtcgcttgcg cccttcacga ccacgccggg
     1681 atcaccggtc gaacgcagcg gacgccgcgg cccgggcgcg ttcggattga tggtctcggc
     1741 gaggtcttca accgcacctt cttcgccacc acgcgcgagc atgaggcgtt ggacgacggt
     1801 ttgtgccgtc acgtgattct caccgactgc ggcgtaaagg ccggacacgt cggtgtagtg
     1861 catgtccctg gcaaggttgc cgagcgcgtc gtcggacaag agccgctgga gtggcaggcc
     1921 ctgcttgcgc atggcgcgcg cgatcgcctg cttgccgtct tcgatcgcgt cctcgcggcg
     1981 ttccttggcg aaccactgcc ggatcttggt gcgggcgcga gggctcttga cgaacgccag
     2041 ccagtcgcgg ctcgggcccg cgttttgcgc cttggaggtg aagatctcga cggcgtcgcc
     2101 gttgtcgagc gtgctgtcga gaggcacaag ccggccgttg acccgcgcgc cgatacagcg
     2161 gtgcccgacc tcggtgtgca ccgcgtaggc gaagtcgact ggccctgagc cttgcggcaa
     2221 cgcgacgacg tcgcccttgg gcgtgaagac gtagacctcg gcgccttgaa tgtcgaaccg
     2281 caacgactcg aggaactcgc ccgggtcggc ggcctccttc tgccagtcaa ggagctgccg
     2341 caaccaggcc atgtcgttcc cggtcgacgg cgactcgacg ccgccggcgg aatcttcctt
     2401 gtacttccag tgcgctgcga tgccgtattc ggcccgccgg tgcatggcgt gggtgcggat
     2461 ctgaagttcg accggcttgc cgtcgggacc gatcaccgtc gtgtgcagcg actggtagag
     2521 gttgaacttg ggcatcgcga tgaagtcctt gaagcggcca ggcaccgggt tccaggtcgc
     2581 gtggatgatg ccgagcgccg cgtagcaatc acggaccgag tcgacgagca cacgcacgcc
     2641 gagcaggtcg tagatgtcgt tcaggtcgcg gccgcgcacg atcatcttct ggtagatcga
     2701 gtagtagtgc ttcggccgac ccgtcaccga cgccttgact ttcgcggttc gaagctgggt
     2761 attgacttgt tcgatcacgc cggcgagata ggtgtcgcgg ctcggcgcgc gatcagtaac
     2821 aagccgcact acctcgtcat accgcttggg aaacagggtt gcgaacgaga gatcttcgag
     2881 ctcccacttc agggtgttca tgccgagccg gtgcgccagg ggtgcgtaga tctcgagggt
     2941 ctcgcgggcc ttgcgctcct gcttctcctc gggcaggaac ccaagagtgc gcatgttgtg
     3001 cagccggtcg gccaacttca gcacgagcac ccgagggtcg cgcgccat
//
LOCUS       DASWVF010000701         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_198055, whole genome shotgun sequence.
ACCESSION   DASWVF010000701 DASWVF010000000
VERSION     DASWVF010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198055"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1568
                     /locus_tag="VGD55_12320"
     CDS             174..1568
                     /locus_tag="VGD55_12320"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY0871177.1"
                     /translation="MHGSSPFPADFVWGVATSAYQIEGAHDVDGRRPSIWDTFCQQPG
                     AIDGGDTGDVACDHYHRWQEDLDLIQQLGVDAYRFSVAWPRIIPTGTGAVNQKGIDFY
                     DALVDGLLARGVTPFVTLYHWDLPQDQQDKGGWPLRDTAYAFADYATVVATALGDRVS
                     NWVTMNEPLCSAWLGHLEGVMAPGVKDIAAAVPASFHLHLGHGLGVQAVRSAAKLTPS
                     IGIVNNLSPVLPASDSAEDIAAARRGDGHTNRWWLDPIHGRGYPQDMVEVYGVEPPVR
                     GNDLDVIAQPLDCVGVNYYFRELTRDNPAGVGPKVAAVRVPGAVETAMGWEVYPQGLE
                     DILVRVADDYAPKKIYVTENGSAWIDKPADDGVVDDVERADYLESHIGACAGARRRGA
                     PVAGYFAWSLIDNFEWAYGYDKRFGLVRVDYDTQARTIKTSGRRFADVIEQHRATTTG
                     ASTTADSASAVGTV"
     gene            1572..>2045
                     /locus_tag="VGD55_12325"
     CDS             1572..>2045
                     /locus_tag="VGD55_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871178.1"
                     /translation="MRIFARRIGFYLLAAVAAITLDFFIPRLVPGNPVLTELAGMKSA
                     PPQALAALELQYGGTHQSMLQQYLHFWNMLLHGNLGYSLSLGNVPVTDIIAQHMWWTV
                     VLVGLSTVISFIVGTTIGTLAAWRRGSWLESLLPITTFLQAMPYFFMATLLVLVFS"
BASE COUNT          365 a          647 c          649 g          384 t
ORIGIN      
        1 ctcgtcattc ctacgacgct cgtagtgagg ggctcgacat agcaacgacg agagcgacat
       61 agccaagcgc ttcatgcgcc cgaagtcaca aatgtgtctc ggctttccgg agccgtggcc
      121 tttacgaccc atgcgcggta acgttcccac ggtcgatcgg agaggaagca ttcgtgcacg
      181 gttcgagccc atttcccgcg gacttcgtgt ggggcgtcgc tacgtcggcg taccaaatcg
      241 aaggcgcgca cgatgtggat ggtcgccgtc cgtcgatctg ggacacgttc tgccagcagc
      301 ccggcgcgat cgacggtgga gacaccggtg acgtcgcgtg cgatcactat caccggtggc
      361 aagaggatct cgacctcatc cagcagctcg gcgtcgatgc ctaccggttc tcggtcgcgt
      421 ggccacgcat cattccgacc ggcaccgggg cggtaaacca gaaggggatc gacttctacg
      481 acgcgctcgt cgacggcctc ctcgcgaggg gggttacgcc gttcgtcacc ttgtaccact
      541 gggatctgcc gcaggaccag caggacaaag gtggctggcc gctccgcgat acggcgtacg
      601 cgttcgccga ttacgccacc gtggtcgcga ccgcgctcgg tgatcgagtc tctaactggg
      661 tgaccatgaa cgagccgctg tgctctgcat ggctcggcca tctcgaaggc gtgatggcac
      721 ccggcgtcaa agacatcgct gctgcagttc cggcttcgtt ccacttgcac ctcggccacg
      781 ggctcggcgt ccaggccgtt cgatctgctg cgaagctcac gccgtctatc ggcatcgtga
      841 acaacttgag cccggtgctg ccggcgagcg acagcgcaga ggacatcgcg gctgcccgtc
      901 gaggcgacgg ccacacgaac agatggtggc tcgacccgat tcacggccgt ggctatcccc
      961 aagacatggt cgaggtctac ggagttgagc cgcccgttcg cggcaacgac ctcgacgtta
     1021 ttgcccaacc gctcgattgt gtcggcgtga actactactt ccgcgagctg acccgagaca
     1081 atccggcagg cgtcgggccg aaggtcgcgg ctgtgcgggt accaggtgcg gtcgagaccg
     1141 cgatggggtg ggaggtctac ccgcaggggc tggaagacat cctcgtgcgc gtcgccgacg
     1201 actacgcgcc aaagaagatc tacgtcaccg aaaacggttc tgcgtggatt gacaagccag
     1261 ctgatgacgg cgtcgtcgac gacgtcgaac gcgctgacta cctcgagtcg cacatcggtg
     1321 cctgcgccgg agcgcgtcgt aggggagcgc cggtcgcggg ctacttcgcg tggtcattga
     1381 ttgacaactt cgagtgggcc tacggctacg acaagcgctt cgggttggtg cgcgtcgact
     1441 acgacaccca agcgcgcacg atcaagacga gcggacgccg gtttgccgac gtcatcgagc
     1501 agcaccgcgc caccacaaca ggtgcgtcaa cgaccgctga ctcggcctcg gctgtgggaa
     1561 cggtctgacc tatgcgcata ttcgcccggc gtatcggctt ttatctgctg gccgcggtgg
     1621 ccgcaatcac gcttgacttc ttcattccgc ggctggtgcc gggaaatccg gtgctgaccg
     1681 agctcgcggg gatgaagagc gcgccaccgc aagcactcgc ggccctcgaa ctgcagtacg
     1741 gcggtacgca ccagagcatg ctccagcagt atctgcactt ctggaacatg ctcctgcacg
     1801 gcaatctcgg atactcgctg agcttgggca atgtgcccgt cacggacatc atcgcccagc
     1861 acatgtggtg gaccgtcgtc ctcgtcggat tgtcgacggt gatcagcttc atcgtgggga
     1921 cgacgatcgg gacgctcgcg gcgtggcggc gaggttcgtg gcttgagagc ctgctgccga
     1981 tcacgacctt cttgcaagcg atgccctact tcttcatggc aacactcctc gtactggtgt
     2041 tctct
//
LOCUS       DASWVF010000702         5195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_427196, whole genome shotgun sequence.
ACCESSION   DASWVF010000702 DASWVF010000000
VERSION     DASWVF010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427196"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGD55_12330"
     CDS             <1..216
                     /locus_tag="VGD55_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0871179.1"
                     /translation="YASSLLNPDADDDAVARVAALVEEAGGRAWTVAECGRRLETAAE
                     VLSTADIEPQAGQALLAIANYVTARDR"
     gene            216..1418
                     /locus_tag="VGD55_12335"
     CDS             216..1418
                     /locus_tag="VGD55_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0871180.1"
                     /translation="MSNGPLVTPGAAASVSASAEAALAATRGDGPPPAQTLLPRWLVT
                     GGAWSWRLIALGIVGYFVLRFILRIELVVLPFLGGMVLAALLRPLASRFQRLGLSRLL
                     AAWATFLIAILVVLGVGTLVVYRSTVEWHTLVSDLSATADKFRHWLSTGPLHLKDQDL
                     KNLQQKGIDQLNQHRGAIVNGVLSGASIIAEVIAGVILTAFITFFLIYDGDRIWRFVS
                     SPFRARTAERVDRAALAAWSTLSGYIRGSVIIATIHGLAMGITLSILGVPLVAPLTLL
                     VFITSFVPLVGVLVGGGLAVFVTMGTQGVTAGIILLAVLVVEHQLEGHLLQPVIMGRY
                     VALHPLAIALALTVGTILEGVVGAIVAVPLVAMLHAAWPHLRESELMVTPDEPPPPGH
                     VLVGDHLN"
     gene            complement(1415..1795)
                     /locus_tag="VGD55_12340"
     CDS             complement(1415..1795)
                     /locus_tag="VGD55_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871181.1"
                     /translation="MPCDAASVAAARQLVRGELTTHAHDVPDRVELVEAASLLVSELV
                     TNAIVHARTDIELRLLADDRMLRAEVTDRNPTLPTRRRPTALTGTGRGLQLIDELASR
                     WGVLVSDAGKTVWFEMTFAIQRTG"
     gene            1964..3130
                     /locus_tag="VGD55_12345"
     CDS             1964..3130
                     /locus_tag="VGD55_12345"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY0871182.1"
                     /translation="MRQVRAGYELRNRGNLLPVAAVVAVASLLMSACTQASHASAIAD
                     KTPTSSPVSAVAASSAAAESAAASEAAAASESALAAGVSGAAGGALGGLDPLTGLPAA
                     SPAAATRPALGVKIDNVEGAWPQAGLNQADIVFDLPVEGGLTRLLAIFHSQDVPLFGP
                     IRSARPVDADLLHLLGHAYFAFSGGTSSDLGPINDHSNATPMWWDVSPSLFVIRHDHA
                     IPHQVFGTTAMLYAAGEQRSPSKTPPPPMFSYATALPTGVSAATNITAQYSAATAIWT
                     WNGKQYLRNQSGHADLLLDGSQVSSTNVVVMSVSVQNTAAHDSHGTVVPLPVVLGSGQ
                     VWVFRNGLVEKGTWSRAGENAPMKLQTSTGQIIALAPGRTWVEVLPNSRLPQIS"
     gene            complement(3134..4402)
                     /gene="kynU"
                     /locus_tag="VGD55_12350"
     CDS             complement(3134..4402)
                     /gene="kynU"
                     /locus_tag="VGD55_12350"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY0871183.1"
                     /translation="MPVADATDRLYAQSLDEADPLREFRLRFLPFPGPDADQLIYLDG
                     NSLGRPPTSALDAITELVATAWAAGVGSWGDWIELPSVVGDVLGAELLGAAAGQVLIS
                     DSTTVNLYKLAAAALDARPDRRTIITEAANFPTDRYVLEGLAAARGLRLDYVDVDVLA
                     GASASDLDLGPDVALVCLSHVDYRSAAIADLAGITRAAHEVGALVLWDLCHSVGSIPI
                     ALDAAGVDLAAGCTYKYLDGGPGAPAFLYVARELQDSLRQPIWGWFGQRDQFAMGATY
                     DPQPSIDRFATGTPNIVGIRLVEAGVRLLAEAGVDRLRAKGIALTTYLIDLFDSWLAP
                     LGFELASPRDSASRGSHISLRHPAAYQLCRALIDNLVVPDYREPDLIRLGVSALTTSF
                     ADVHEAMRRLHSLTVAGAYLDIETVKSRVT"
     gene            complement(4405..4704)
                     /locus_tag="VGD55_12355"
     CDS             complement(4405..4704)
                     /locus_tag="VGD55_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEY0871184.1"
                     /translation="MSDIEELPHREAPGIGTLTMPETDARPVPRLSHGDGDHERFAHY
                     APKDKIVEASVMGTPITALCGKVWVPSRDPKRFPVCPECKDIYENVLTGGDQPAQ"
     gene            complement(4750..4941)
                     /locus_tag="VGD55_12360"
     CDS             complement(4750..4941)
                     /locus_tag="VGD55_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871185.1"
                     /translation="MSSTAKTETVTSLSGLGASAFTVTKGGKIVGVDTITSGALLVSL
                     AGVFTGDVRVIPERLIALY"
     gene            4946..>5195
                     /locus_tag="VGD55_12365"
     CDS             4946..>5195
                     /locus_tag="VGD55_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871186.1"
                     /translation="MANAAGVLGPMLRMKTTVLSAEANSQLRCEGEDVGNDVFKLATA
                     GANTATGIAAHDVGPAPGAAAPAGAADGVDVDFALRADA"
BASE COUNT          898 a         1716 c         1697 g          884 t
ORIGIN      
        1 tacgcgtcga gcctgttgaa ccccgacgcc gacgacgacg ccgtagcccg agtagccgcc
       61 cttgtcgaag aagccggcgg ccgcgcctgg accgtcgccg aatgcgggcg tcgcctggag
      121 accgctgcag aagttctgtc gactgcggat atcgagccgc aagcggggca ggctctgctc
      181 gcgatcgcaa actacgtgac ggccagggac cgctgatgtc gaacggacca ctcgtaacac
      241 ccggcgctgc ggcatctgtg tctgcctccg ctgaagcggc gctggccgcg accagaggcg
      301 acggtccgcc gcctgcgcag acgttgctcc cgcgctggct cgtgaccggc ggcgcgtggt
      361 cgtggcggct gattgccctt ggcatcgtgg ggtacttcgt tctgcgattc atccttcgca
      421 tcgagttggt cgtgctcccg ttcctgggcg gcatggtctt ggccgcgctc ttgcgtccac
      481 tcgccagtcg cttccaacgg ctcggccttt cgcggctgct cgcagcgtgg gcgaccttcc
      541 tgatcgcgat cctcgtcgtc ctcggcgttg gcacgttggt ggtgtatcgc tcgacggtcg
      601 agtggcacac gctcgtcagc gacttgtcgg cgacggccga caagttccga cactggttgt
      661 cgacggggcc gctgcacctg aaggaccaag acctgaagaa tcttcagcaa aagggcatcg
      721 accagttgaa ccagcaccgc ggagccatcg tcaacggcgt cctgagcgga gcgagcatca
      781 tcgctgaggt catcgccggc gtcatcttga cggcgttcat cacgttcttc ctcatctacg
      841 acggcgaccg catctggcga ttcgtgagct cgccgtttcg ggcgcgcacg gccgagcgcg
      901 tcgaccgagc cgcacttgcc gcgtggagca cgttgtcggg ctacatccgc ggcagcgtca
      961 tcatcgccac gatccacggg cttgcgatgg gcatcacgct gtcgatcctc ggcgtcccgc
     1021 ttgtcgcgcc gttgacgttg ctcgtcttca tcaccagctt cgtgccgctg gtcggagtac
     1081 tcgtcggcgg tggcttggcg gtgttcgtga cgatgggcac ccagggcgtg accgccggca
     1141 tcatcttgct cgcggtcctc gtggttgagc atcagctaga gggccacctc ctccagcccg
     1201 tcatcatggg ccgctacgtc gccctgcacc cactcgcgat cgccctcgcg ctcacggtcg
     1261 gaacgatcct cgaaggtgtc gtcggcgcga tcgtggcggt accgctggtc gcgatgctgc
     1321 acgcggcgtg gccgcacctg cgcgagtcag aactcatggt cacacccgac gagccaccac
     1381 caccaggaca cgtgcttgtc ggtgaccatc tgaactagcc ggtgcgttgg atcgcgaacg
     1441 tcatctcaaa ccacaccgtc ttgccggcgt ccgacaccaa cacaccccac cggctcgcca
     1501 gctcgtcgat gagttgaagg ccacggccag tgccagtcaa tgcggtcgga cgccgacggg
     1561 tcggcaacgt cggattgcga tcggtcacct ctgctcgcaa catgcgatcg tcggcgagca
     1621 gccgcaattc gatatcggta cgcgcatgga cgatcgcgtt tgttaccagc tcgctgacca
     1681 gcaggctcgc tgcttcgacg agttcgacgc gatcaggtac gtcgtgcgcg tgagtggtca
     1741 gttcgcccct gaccagctgg cgagccgcgg caacgctggc agcgtcacaa ggcaagacag
     1801 cccggacgtc cacatcgaaa acgatacgag gatcaccgcc ggcaaccttg cgactcgccc
     1861 gcgcgttctc ttcggtaacg cgggctagcc acccgccacg acagttcggc aggtagggtc
     1921 ggccggaatc aagacgcgag cgagcacagg aagcaggcac cacgtgcgac aggttcgcgc
     1981 cggatatgag ctgagaaatc ggggcaactt gctgccagtc gcagcagttg tcgccgtggc
     2041 cagcttgctc atgagcgcgt gtacgcaggc ctcacatgcc agtgcaatag cggacaaaac
     2101 gccgaccagc tcgccggtgt ccgccgtcgc ggcaagttcg gcggccgccg agtcggctgc
     2161 cgcttccgag gccgcggcag cctcggagtc ggcgctcgca gcgggggtgt cgggcgcggc
     2221 aggcggtgcg ctcggcggtc ttgacccact caccgggttg ccggcggcga gtccggcggc
     2281 ggcaactcga cccgcgcttg gtgtgaagat cgacaatgtc gagggcgcct ggccgcaggc
     2341 cggtctcaac caggccgaca tcgtgttcga ccttcccgtc gagggcggcc tgacccgcct
     2401 tctggcgatc ttccactccc aagacgtgcc actcttcgga ccgatccgct ccgcccggcc
     2461 ggtcgacgcc gatctgctgc acctactggg ccacgcctat ttcgccttct ctggcggcac
     2521 gagttcagac ctcggcccga tcaacgacca ctcgaatgcg accccaatgt ggtgggacgt
     2581 ctcgccgtcg ctgttcgtga tccgccacga ccacgcgatc ccccatcagg tgttcggcac
     2641 caccgcgatg ctgtacgcag cgggcgagca gcgatcgccg tcgaagaccc cgcccccgcc
     2701 gatgttcagt tacgccaccg cgcttccgac tggcgtatcg gcggcgacga acatcacggc
     2761 gcagtacagc gcggccaccg cgatctggac ctggaacggc aagcagtacc tgcgcaacca
     2821 gagcggtcac gccgacctgc tgctcgacgg cagccaagtc tcgagcacga acgtggtcgt
     2881 gatgtcagtg tcggtgcaaa atactgcagc acacgactcg cacggcacgg tggtcccact
     2941 gccggtcgtg ctcggctccg ggcaggtgtg ggtcttccgc aacggcctgg tcgagaaggg
     3001 cacctggagc cgcgccggcg agaacgcacc gatgaaactc cagacgtcga caggccagat
     3061 catcgcgctg gccccgggtc gcacctgggt tgaggtgctc ccgaactcgc ggctgccgca
     3121 gatcagctga cggctacgtc actcgtgact tgactgtctc gatatcgaga taggcacctg
     3181 cgactgtcaa cgagtgcaga cggcgcatcg cctcgtgcac gtcggcaaaa ctcgtcgtca
     3241 gtgcggagac accgagcctg atgagatcag gctcccggta gtcaggcacg acgagattgt
     3301 cgatgagggc gcgacatagc tgatacgcag cggggtggcg cagcgagata tgcgatccgc
     3361 gagacgccga gtcgcgcggg ctcgcaagtt cgaagcccag cggcgcgagc cagctgtcga
     3421 acaagtcgat cagataggtc gtcaacgcaa tgcctttggc acgcagtcga tcgacacccg
     3481 cctcggcaag caaccgcacg cccgcttcga ccagcctgat gccgacgatg ttcggagtgc
     3541 cagtcgcgaa tcggtcgatc gacggttgcg gatcgtacgt cgcgcccatc gcgaactgat
     3601 cgcgctggcc gaaccatccc cagatcggtt gtcgcaatga gtcctgcaat tcccttgcca
     3661 cgtagagaaa agcaggcgca cccggcccac cgtcgaggta cttgtacgtg cagccggctg
     3721 cgagatctac acctgcggca tcgagtgcga tcggtataga gccgaccgag tgacacaggt
     3781 cccacaacac cagcgcgccc acctcgtgtg cggcgcgcgt gatgccagcc aggtcagcga
     3841 tggcagccga tcggtagtcg acgtgcgaca ggcacaccag cgcgacgtca ggaccaagat
     3901 cgaggtcgga cgccgaagcg ccggcgagta cgtcaacgtc gacgtaatcg agccgaaggc
     3961 ctcgtgccgc tgcgagtcct tcgagcacgt accgatcggt cgggaagttc gcggcttcgg
     4021 tgatgatcgt gcgccgatcc ggccgggcgt ctagtgctgc cgcggcgagc ttgtagaggt
     4081 tgaccgtggt cgagtcgctg atgagcacct gccctgcggc tgcgccgagc agctcagcgc
     4141 caagcacgtc gccgacaacg cttggcagtt cgatccagtc gccccacgat ccgacgccgg
     4201 cggcccaggc ggtcgcgaca agttcggtga tggcgtcgag cgctgaagtc ggcggcctgc
     4261 caagggagtt accgtcgaga tagatcagct gatcggcgtc cgggcctgga aaaggcagga
     4321 accgcagccg aaattcgcgg agcggatcag cttcgtcgag agattgtgcg tagagacggt
     4381 cggtggcgtc ggcaacgggc atcgctactg cgccggctgg tcgccgccgg tgagcacgtt
     4441 ctcgtagatg tccttgcact cgggacacac cgggaaccgt ttgggatcgc gcgacggaac
     4501 ccagaccttg ccgcacagcg cggtgatcgg cgtgcccatg acggacgcct cgacgatctt
     4561 gtccttcggt gcgtagtgcg cgaatcgctc gtgatcgccg tcgccgtgcg acagtcgcgg
     4621 caccggccgc gcgtcagtct cgggcatcgt cagcgtgccg atgccgggcg cttcgcggtg
     4681 cggcagttct tcgatgtcgc tcatgcggag cagtctaaag gagcccggag atctcgtgga
     4741 ccctgatcgc tagtacagcg cgatcaagcg ctcgggaatc acccggacgt ctccggtgaa
     4801 gacaccggcg agtgacacga gcagcgcgcc tgaggtgatg gtgtcgaccc cgacgatctt
     4861 gccgcccttg gtgaccgtga atgccgacgc accgagacca ctgagcgacg tgaccgtctc
     4921 ggtcttcgcc gtagaggaca tgagcgtggc gaacgccgcc ggtgtgctcg gcccgatgtt
     4981 gaggatgaag accaccgtgt tgtccgccga agcgaactcg caactcagat gcgagggcga
     5041 ggatgtcgga aacgacgtct tcaagctcgc gacggccgga gcgaatactg cgaccgggat
     5101 agcggcgcac gacgtcggtc cggcaccggg cgcggccgca cccgccggag cagcggatgg
     5161 cgtggacgtc gactttgcgt tgcgagctga cgctc
//
LOCUS       DASWVF010000703         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_44834, whole genome shotgun sequence.
ACCESSION   DASWVF010000703 DASWVF010000000
VERSION     DASWVF010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44834"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="dprA"
                     /locus_tag="VGD55_12370"
     CDS             complement(<1..827)
                     /gene="dprA"
                     /locus_tag="VGD55_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0871187.1"
                     /translation="MSDDQHPNPMTTSGVGDHNDRIARLLLGRVSEPGDVALGRVIAD
                     LGAVGTVQRLRAGTLTHNGLVNLRARLDVARPDRDLEAGAVVGARFVIPGDAEWPDQV
                     DDLVRLGRRDAGVPLGLWVRGPLNLRVACRRSVAVVGARASTAYGDYVASEFGIGLAE
                     RAWTVVSGGAYGIDGAAHRGALAGEGPTVAVLACGVDIAYPRGHSALIDRIAQTGLVV
                     SEWPPGCAPMRYRFLVRNRVIAALTVGTVVVEAAIRSGALSTANRARELDRHIMSVPG
                     "
     gene            complement(824..>1898)
                     /locus_tag="VGD55_12375"
     CDS             complement(824..>1898)
                     /locus_tag="VGD55_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871188.1"
                     /translation="ELVVALRSGRRPEMALDRPVGDDAPLADASAAKEHFDIELDLSD
                     VLGQPSGRRAVEVAAAGGHHALLLGDPGTGKTMLAARLPGLLPELDRVAALEVTAVHS
                     IAGVLPGDCPLVTRPPFRDPHHTTSVAALVGGGSGMARPGVASLAHHGVLFLDEAPEF
                     AAGVLDALRQPLESGHVTIARAGGTARYPARFLLVLAANPCGCSTSSAKPGSCTCSPL
                     IRRRYLNRISGPLLDRVDLQVRMDPVSRAELLADRHHVEHSATVAGRVIVARSRAAER
                     LAGSPWRTNSDVPGRELRDRWAPARTALVAAERALDAGRLSARGLDRVLRVAWTLADL
                     ADRTRPTADDIDEALYLRTGVAS"
BASE COUNT          341 a          676 c          635 g          246 t
ORIGIN      
        1 cccggcacgg acatgatgtg ccggtcgagc tcacgtgctc ggttcgcggt gctgagcgcg
       61 ccgctgcgaa tggccgcctc aacgacaacc gtgccaaccg tcagcgcggc gatgacacgg
      121 ttgcgcacca ggaatcggta gcgcatcggc gcgcagccgg gaggccactc actcacgacc
      181 aggccggtct gcgcgatccg gtcgatgagc gccgaatgcc cgcgggggta cgcgatgtcc
      241 accccgcagg cgagcactgc aacggtgggc ccctcgccag caagtgctcc ccgatgggcg
      301 gcaccatcga tgccgtaggc accgcccgat acgacggtcc atgcgcgctc ggcgagcccg
      361 attccgaact cgctggcgac atagtcgcca tacgcagtcg acgcgcgagc acccacgacg
      421 gcaaccgagc gacggcaggc gacccgcaga ttgagcggtc cgcgaaccca caagcccagc
      481 ggcacgcctg cgtcacggcg accgagccga accaggtcat cgacctggtc gggccactcc
      541 gcgtcgcccg gaatgacgaa cctcgcacca accaccgccc ccgcctcgag gtcgcgatcg
      601 ggacgtgcga cgtcgagacg cgcacgcagg ttcaccagtc cgttgtgggt cagcgtgccg
      661 gcgcggagtc gttgcacggt gccgaccgcg ccgaggtcgg cgatgaccct tcccagcgcg
      721 acgtcgccgg gctcggagac ccgacccagc agcagccgag cgattcgatc gttgtggtcg
      781 cccacccctg atgtggtcat cgggttcggg tgctggtcgt cgctcacgag gcaacccccg
      841 tgcgcaggta gagcgcctcg tcgatgtcgt cggccgtagg gcgtgtgcgg tcagcaaggt
      901 cggcgagcgt ccacgcgacg cggagtaccc gatcaaggcc acgagcagac aaccgacccg
      961 cgtcgagtgc gcgttcggca gcgaccagcg ccgtgcgggc aggagcccat ctgtcgcgca
     1021 gttctcgacc tgggacgtcg ctgttcgttc gccacgggct acccgccagg cgctcagcag
     1081 ccctcgaccg cgcgactatc actcggccag cgacggtcgc actgtgctcc acgtggtggc
     1141 gatcggccaa cagttccgcg cggctcactg gatccattcg cacctggaga tcaacccggt
     1201 caagcaacgg accggagatg cggttcaggt aacgacgccg gatcagcggt gagcaggtgc
     1261 acgagccggg cttcgcactc gatgtcgaac agccgcaggg attcgcggcg agcaccagca
     1321 ggaaacgcgc cggatagcgg gcggtgcccc cagcccgcgc gatcgtcacg tgacccgatt
     1381 cgaggggctg ccgcaacgcg tcgagcacgc cggccgcgaa ctcgggagcc tcgtccaaga
     1441 aaagaacccc gtgatgcgca agcgacgcaa caccgggtcg ggccatgccg ctgccgccac
     1501 caacaagagc ggcgacggac gtcgtgtggt gcgggtcgcg aaacggcggc cgagtaacta
     1561 acggacaatc gcctggcaac acccctgcga tcgagtgcac cgccgtgact tcaagtgccg
     1621 cgacccgatc caactcgggc aacaacccag gcaaccgagc agcgagcatc gtcttgccgg
     1681 tacctgggtc gccgagcagc aacgcgtgat ggccgccggc cgccgcgacc tcgaccgccc
     1741 ggcggcccga cggctgaccc aaaacgtcgg acagatccag ctcgatgtcg aagtgctctt
     1801 tcgcggccga cgcgtcggcg aggggggcgt cgtcgccgac cggacgatcg agggccatct
     1861 ccggccgtcg gccgctgcgt aacgcaacaa cgagttcg
//
LOCUS       DASWVF010000704         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_198082, whole genome shotgun sequence.
ACCESSION   DASWVF010000704 DASWVF010000000
VERSION     DASWVF010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198082"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGD55_12380"
     CDS             <1..499
                     /locus_tag="VGD55_12380"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEY0871189.1"
                     /translation="WIDVIRASGKTKQADVVAMLKDDYALTHGAAHRVSLVAREALGT
                     QQTRGDSETNVLDGLYAGKKTALRPIHNKLLAAVYALGKEIEIAPKKGYLSLRRRKQF
                     AMIKPAAGHVDVGLILDDAATTERLESAATFNALFTHRVRLREPRDVDGELTAWLKRA
                     YTEAK"
     gene            848..1156
                     /locus_tag="VGD55_12385"
     CDS             848..1156
                     /locus_tag="VGD55_12385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY0871190.1"
                     /translation="MKAKKTDVIVLATARAKPGKESDLEDALRECAGPTREQAGCVQF
                     FLLRAADDKSTIVGYERWVSEADHQRHLKGAHIQRLMARMADILSGPPEIVAYETIDE
                     "
     gene            1242..1664
                     /locus_tag="VGD55_12390"
     CDS             1242..1664
                     /locus_tag="VGD55_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0871191.1"
                     /translation="MATIKKDRAEVFRALTTQTGLSSWWSTEVTADKPVGGVIRFRFL
                     GDFHPELKIVALEEPSLVTWRCVAGHDNWRDNEFEFRLEDVSDDTRLKFVQTYSRELD
                     DVQYGTYNFNWGYYLESLRLYCEAGRGKPFDPKTGRSG"
     gene            1762..2067
                     /locus_tag="VGD55_12395"
     CDS             1762..2067
                     /locus_tag="VGD55_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0871192.1"
                     /translation="MVTIIVNFNIKADRMDEATACIARFVQRIEQNEPETLTYRSYQD
                     TSNPLHFLHFMEFKDEQAYERHRTSAYLKEFVGILYPCCSESPPTQPWYLNAFKRKD"
BASE COUNT          450 a          672 c          732 g          365 t
ORIGIN      
        1 atggatcgac gtcatccgcg catcgggcaa gacgaagcag gctgatgtcg tcgcgatgct
       61 gaaggacgac tacgccctga cgcacggtgc ggcgcatcga gtgtcgctcg tcgcccgcga
      121 ggcactcgga acgcagcaga ccaggggcga ttcggagacg aacgtccttg acgggctcta
      181 cgccgggaag aagacggcgc tccggccgat ccacaacaag ctgctcgccg ccgtctacgc
      241 cctcggcaag gagatcgaga tcgcgcccaa gaaggggtac ttgagtctca ggcgacgcaa
      301 gcagttcgcg atgatcaagc ccgccgccgg tcacgtggac gtcggcctga tcttggatga
      361 tgcggcgacg acggaacgcc tcgagtccgc ggccacattc aacgcgctct tcactcatcg
      421 tgtacggctt cgcgagccac gcgacgtgga cggcgagctc acagcttggt tgaagcgggc
      481 gtacacggaa gcgaaatgac gtacgcatcg cctggagtgg cgcgggccat gtccatctct
      541 cacggacagg ctcgagacag cgcggacgac ggtcggttac ggcgccgcgg gccgcttcaa
      601 cgggcgggtc cggtctcacg aacacccgta tcctcggccg tggattggca tttccgcatc
      661 ggcgtcgtcg cgaagatccc ccgctggcag gctcgggcag acggcagaca tggcgaaggc
      721 tgcgctctac ctcacgtcgg acgactcctc gtagtcgtcg gggacagagc tggcgtcgac
      781 ggccggtcgg gcgcaacgct gaaaagcagt tccctcacgc aactccacga acaagaagag
      841 gctgaccatg aaagcgaaga agacggacgt tatcgtcctc gcaaccgcac gagccaagcc
      901 cggtaaagag agcgacctgg aggacgcgtt gcgcgaatgc gcaggcccca cccgtgagca
      961 ggccggttgc gtgcagttct tcctgctccg tgccgcggac gacaagtcaa cgatcgtcgg
     1021 ctacgagcgc tgggtctcgg aggccgacca ccagcgccat ctcaaggggg ctcacatcca
     1081 gcggctgatg gcccggatgg ctgacatcct ctccggaccg ccggagatcg tcgcctacga
     1141 gacgatcgac gaatagctag catggcgagt ctcacgccgc acgggcgcag gtggcatgcg
     1201 ttgaaatggg gagggagctc aagttgaaaa cgataattca cgtggcaacg atcaagaagg
     1261 accgggccga agtgtttcgc gctctgacta cgcagactgg cctctcgtcg tggtggtcga
     1321 ccgaggtgac cgcggacaag ccggtgggtg gcgtaatccg gtttcgtttc cttggggact
     1381 tccacccgga gctgaagatc gtggccttgg aggagccttc gctcgtcacg tggcgctgcg
     1441 ttgctggtca cgacaactgg cgcgacaacg agttcgagtt tcgtctcgag gacgtcagcg
     1501 acgacacgcg actcaaattc gtgcagacct actcgcgcga gctcgatgac gtgcagtacg
     1561 ggacctacaa cttcaactgg ggctactacc tcgagagtct tcgcctctac tgcgaggccg
     1621 gtagggggaa gccgttcgac ccgaagacgg ggcgatcggg ctgagcactg gagcgtgggc
     1681 gcgctcgacg agtggcggac gcgcacgaaa gcgacgggtc gcgtgggatg aactgttggt
     1741 agccaaacgc ggggtgcaga cgtggtcacg atcattgtca acttcaacat caaggcggat
     1801 cgcatggacg aggcgacggc ctgcatcgct cgcttcgttc agcgcatcga gcagaacgag
     1861 cctgagacgc tcacgtaccg ctcgtaccag gacacgagca acccgctcca cttcctccac
     1921 ttcatggagt tcaaggacga gcaggcctac gagcgtcacc ggacgagcgc gtacctcaag
     1981 gagttcgtcg gcattctgta cccatgctgt tcggagtcgc ctccgacgca gccttggtac
     2041 ttgaacgctt tcaaacggaa ggactaggtt atggccgcca gacgacgggg gcaaggcgga
     2101 gcgacgacgt tctcgcaggc aggcgctggc aaacccccga tcagctgtca cgtttcgtgg
     2161 tgtcgaggag tcacatgagg ctcgaggacg aggtcgccgt cgtcaccggg ggtaacagc
//
LOCUS       DASWVF010000705         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_503850, whole genome shotgun sequence.
ACCESSION   DASWVF010000705 DASWVF010000000
VERSION     DASWVF010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503850"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGD55_12400"
     CDS             complement(<1..816)
                     /locus_tag="VGD55_12400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0871193.1"
                     /translation="MTALVEAPLRKLDDAPVIAPAKRRHPRVWIGLALFAICSGHALL
                     TLRLHNGVFEDEGLYIYQGHRAIAHLLHGVRLSDNPGSYLSGAPALYPVYAALLDHLG
                     GLEAVRFGSLPFTLLGICCVYAIGRRLFSTAAGLLGGAALAVCGPAIFLSHFATYDAA
                     IVGLVALATWLAIRSAQDDGLLWAPMIAAILVLAGFTKYAAFAYVPMVFALGVVVGWR
                     VYRWRVVRRAVFGLLCAVALFFVVIEVWGRSLIPGIELTTTNRKVLGPTSAHVL"
     gene            complement(813..2444)
                     /locus_tag="VGD55_12405"
     CDS             complement(813..2444)
                     /locus_tag="VGD55_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase catalytic subunit"
                     /protein_id="HEY0871194.1"
                     /translation="MNTLHGLLLPSDQAKHAGLRDRLPTPPTDKEKYAYFGPQRRWIV
                     FVQFAGFSSIAASVTMFMVRTGWTWFVLGPPLALTILASICSLLTSSRGRRFDIAYYE
                     SVVANWSASANYSVDVFLPSAGESLNLLRNTYRYVSQLHWLGPLTIYVLDDSGRPDVE
                     TLAREFGFTYLSRPDRGRMKKAGNLAFGYEHSSGDLIVVFDADFCPRSDFLDSTVPFM
                     RDSVIGIVQTPQYFDVDPSQNWLQRGAGATQEFFYRWVQPARDRSDGAICVGTNAVYR
                     RKALAKAGGFAQIGHSEDVHTGVNLLRVGYLTRYVPVVVAKGACPDAINAFVVQQYRW
                     CTGSMSLLFSRTFHHTPLTTRQRLCFWTGFLYYITTAINVFAMWLPPLIVGFIYPHKM
                     HPSNYVLVIVAVLVRMSLIPVLTSERGTWAGLARIQMLYSFAHATALIHSVRGRSVSW
                     VATGAARGTGLSRQIRMLTVGWLVSCQALLWLAILRDVPRYGISNYWPMVVFASINLY
                     LVAPIVGGLTPRTLAARRAVRRSVMLRATAPDGGQ"
     gene            complement(2586..>2824)
                     /locus_tag="VGD55_12410"
     CDS             complement(2586..>2824)
                     /locus_tag="VGD55_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871195.1"
                     /translation="AITEWGVTQLTNGHGGGDDPAFVDRMFDFMTDPANNVAYAHYFN
                     TGSVADDHTLTGSTKFPESAAEYRDRVQALAGPK"
BASE COUNT          591 a          890 c          909 g          434 t
ORIGIN      
        1 cagcacgtgc gcgctcgtcg ggccgagcac cttgcggttc gtggtcgtca attcgatgcc
       61 ggggatcagc gaccgtcccc agacctcgat cacaacgaag aacaacgcaa ccgcgcagag
      121 cagtccgaat accgctcggc gaacaacccg ccagcgatag acccgccacc ccaccacgac
      181 gccgagtgcg aacaccatcg gcacgtaggc gaacgccgcg tatttggtga agccggctag
      241 aacgaggatc gcggcaatca tgggcgccca cagcagcccg tcgtcctgcg cgctgcgaat
      301 ggccagccac gttgcgagcg cgaccaagcc gacgatcgcc gcgtcgtacg tcgcgaaatg
      361 tgagaggaag atggccgggc cgcacactgc cagcgcggcc ccaccgagaa ggcccgctgc
      421 cgtgctgaac aagcgccgcc cgatggcgta gacgcagcag atccctagca gcgtgaacgg
      481 gaggctgccg aaccgcactg cctcgagccc gccgaggtga tcgaggagcg ccgcataaac
      541 cgggtacagc gcaggggcac cagacagata agagcctgga ttgtccgaca gccgcacgcc
      601 gtgcaggaga tgcgcgatcg ctcgatgccc ttggtagata tacagtccct cgtcttcgaa
      661 gacgccattg tgcagacgaa gtgtcagaag ggcgtggcct gagcagatcg cgaacagcgc
      721 aaggccgatc cacactctcg ggtgccggcg cttcgcaggc gcgatgaccg gtgcgtcatc
      781 gagtttgcgc aacggcgcct cgaccagtgc ggtcactgcc cgccgtccgg cgcagtcgca
      841 cggagcatca cagacctacg aacggcgcgg cgggcggcga gcgttcgagg agtgagacct
      901 cccacgatgg gtgccacgag gtagagattg atgctcgcga agaccaccat cggccaatag
      961 ttcgagatgc cgtaccgcgg cacgtcccgg aggatggcga gccacagcaa ggcctgacac
     1021 gacaccaacc agccgacggt cagcatgcgg atctgtcgcg aaaggccggt tccgcgggcc
     1081 gctcccgtgg ccacccagct caccgaccgc ccacgcaccg aatgaatcag cgcggttgcg
     1141 tgcgcgaagc tgtagagcat ctggatccgc gcgagccctg cccacgtacc ccgctcagac
     1201 gtaagtaccg gaatcaacga catcctgacg agaacggcca cgatcaccag cacgtagttc
     1261 gacggatgca tcttgtgcgg atatataaat ccgacgatca acggcggtag ccacatcgcg
     1321 aacacgttga tcgcggtggt gatgtagtac aggaagccgg tccaaaagca gagtcgctgt
     1381 cgggttgtca atggcgtgtg atgaaacgtc cgcgagaaga gcaggctcat cgagccggta
     1441 caccaacgat attgctggac gacgaatgcg ttgatcgcgt cgggacacgc gcccttcgcc
     1501 acgacgaccg ggacgtaacg cgtgaggtag ccgacacgca acaggttgac accggtgtgc
     1561 acgtcctcgc tgtggccgat ctgcgcaaag ccgccagcct tggcgagtgc cttccggcga
     1621 tagaccgcgt tggtgccgac gcagattgcg ccatctgaac gatcacgcgc cggctgcacc
     1681 caccggtaga agaactcctg ggtggcgcca gccccgcgtt gtagccagtt ctgcgacgga
     1741 tcgacgtcga agtactgcgg ggtctgcacg atgccgatga ccgagtcacg catgaacggc
     1801 acagtggagt cgaggaaatc cgagcgcgga cagaagtctg cgtcgaacac gacgatcaag
     1861 tcgccgctcg aatgctcgta gccgaaggcg agattgcccg ccttcttcat gcgtccacgg
     1921 tcaggccggc tgaggtaggt gaagccgaac tcgcgcgcaa gcgtctcgac gtcggggcga
     1981 ccggagtcgt cgagcacgta gatcgtcaaa ggcccgagcc agtgcagctg cgagacgtag
     2041 cggtacgtgt tgcgcagaag gttcagcgat tcgcccgcgc tcggcaggaa cacgtcaacg
     2101 ctgtaattgg cggacgccga ccagttggcc acgaccgatt cgtaatacgc gatgtcgaac
     2161 cgacgacctc gcgacgatgt caggagcgag cagatgctcg cgaggatcgt gagcgcgaga
     2221 gggggcccga gcacgaacca ggtccagccg gtccgcacca tgaacatcgt gacgctcgcg
     2281 gcaatggagc tgaatcccgc gaactgaaca aagacgatcc agcgccgttg gggtccgaag
     2341 taggcgtact tctccttgtc ggtcggcggc gtcgggagcc ggtcgcgaag gcccgcgtgt
     2401 ttggcctggt ccgagggcaa caggaggcca tgcagcgtgt tcacggctac ctccgcagga
     2461 ctgccgaaac cccatctgca gcttcggccc cagggccctc attgagagca cgacactaaa
     2521 cggtcttagg ccctcaaagc gatatctatc aggttaaatt ttcgatcgct ccgggcggat
     2581 tgtgatcatt taggccccgc gagggcctga acccggtcgc ggtactcggc cgcagactcg
     2641 gggaacttgg tgctccccgt cagcgtgtgg tcgtcagcca ccgacccggt gttgaagtag
     2701 tgcgcgtacg cgacgttgtt ggccggatcg gtcatgaagt cgaacatgcg atcgacgaac
     2761 gccgggtcgt cgccgccgcc gtgcccgttc gtcagttggg tgacacccca ctcggtgatg
     2821 gcca
//
LOCUS       DASWVF010000706         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_325110, whole genome shotgun sequence.
ACCESSION   DASWVF010000706 DASWVF010000000
VERSION     DASWVF010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325110"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1286)
                     /locus_tag="VGD55_12415"
     CDS             complement(90..1286)
                     /locus_tag="VGD55_12415"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0871196.1"
                     /translation="MQQQRERVDVPLRLGDRGLAVVELRHRLRQLELLPSDEVGGGAA
                     AVDDFDDACDLAVRSFQQQRGLRIDGVVDRETYRGLDEARWRLGDRPLSFAVRHPYVG
                     DDVAGLQHRLIDMGFDCGRADGVFGPRTENALREFQRNVGLPADGACGPATFRALRQL
                     ARTVVGGKANELREYERLQHGGPTMAGKLVIIDPGHGGADRGSTNGMLDEAGLVDDIA
                     NRVEGRLAAAGALAYRTRGGEGSTQSPGDLELLDRLTSDAERGAFANAAEADLLVSIH
                     VDGHANPQCRGVSTYYYGTVNDRSLVGARLAELIQQEILARTDLLDCRSHPKTWELLR
                     RTRMPAVRIELVYLTNPGDAARLGRPEFRDELAEAIVVAVQRLFSPAELPSRESIELS
                     RQTISV"
     gene            complement(1346..1669)
                     /gene="trxA"
                     /locus_tag="VGD55_12420"
     CDS             complement(1346..1669)
                     /gene="trxA"
                     /locus_tag="VGD55_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0871197.1"
                     /translation="MGATKSVTDETFATDVLASDTPVLVDFWAEWCGPCRMVAPVLEE
                     IANEHADKVRVVKLNIDENPETARAYSILSIPTLNVYQGGQVVKQIIGAKSKSALLKD
                     LEAFL"
     gene            complement(1723..>2254)
                     /locus_tag="VGD55_12425"
     CDS             complement(1723..>2254)
                     /locus_tag="VGD55_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0871198.1"
                     /translation="FRDQDIAVVGGGDSALEEATFLSKFARSVTVVHRRDELRASKIM
                     QDRAKANDKVGFRWNTEVVDVIGDDKVAGLTVRDTVTGADDQLPVTGLFVAIGHDPRS
                     ELFRGQVDLDAAGYVKVQQPSTRTNLDGVFACGDLVDHTYRQAITAAGTGCAAALDAE
                     RWLAAQQAALKPAFAE"
BASE COUNT          368 a          711 c          766 g          409 t
ORIGIN      
        1 gacgtggcgt gaggacgtcg agggtgcgct cgagcggttg ctcgggtcgg ttgcgcctga
       61 gggtgcactt cggcccgtgt gatcggtagc tacaccgaaa tcgtctgccg gctcagttcg
      121 attgattcac gcgacggcag ctcggccgga gagaacagtc gttgaacagc gacgacgatt
      181 gcctcggcaa gctcgtcgcg aaactcgggc cggccaaggc gtgcggcatc acctggattg
      241 gtgaggtaaa ccaactcaat gcggaccgcg ggcatcctgg tgcggcgtag gagctcccag
      301 gttttcgggt ggctgcggca gtcgagcagg tcggtgcgcg ccaggatttc ttgctggatc
      361 aactcggcaa ggcgggcgcc gacaagtgac cggtcattga cagtgccgta gtagtacgtc
      421 gagactccgc ggcactgcgg gttcgcgtga ccgtcgacgt ggatggagac caggagatct
      481 gcctcggctg cgttggcgaa cgccccgcgc tcggcgtcac ttgtcagccg gtcgaggagt
      541 tcgaggtcgc ccgggctctg cgttgacccc tcgccgccgc gggttcggta cgcaagggca
      601 ccggcagctg cgagccgacc ctcgaccctg ttcgcgatgt cgtcgacgag gccggcctcg
      661 tcgagcatgc cgttggtgga tccacggtcg gctccgccgt ggccgggatc gatgatgacg
      721 agtttgcccg ccatcgtcgg accgccgtgt tggagccgct cgtactcgcg caactcgttc
      781 gccttgccac ctaccacggt tctggcgagc tggcgaagcg ctcgaaacgt cgcggggccg
      841 caggcgccgt cagctggcag acccacgttg cgctggaact cgcgcaacgc attctcggtt
      901 cgcggaccga agacgccgtc ggcgcggccg cagtcgaagc ccatgtctat taagcggtgc
      961 tgtagcccag caacgtcgtc gcctacgtag ggatgccgca cagcgaaaga cagcggccga
     1021 tcgcccagcc gccagcgcgc ttcgtcgagg ccgcggtagg tctcgcgatc gacgaccccg
     1081 tcgatgcgca agccacgctg ctgctgaaaa cttcggaccg ccaggtcgca cgcgtcgtcg
     1141 aagtcatcga ccgccgcggc accgccaccg acctcgtcac taggcagcag ctcgagctgg
     1201 cgtagccggt ggcgtagctc gaccacagca agaccgcggt caccgagccg caagggcacg
     1261 tcgacgcgct cgcgctgttg ttgcatgggg gtcctcatgg ctcatctacc tgggactacc
     1321 gggccggaag agccatgcta gcgggctaca ggaacgcttc cagatccttg agcagagccg
     1381 acttcgactt ggcgccgatg atctgcttca cgacctggcc tccttgatag acgttgaggg
     1441 ttgggatcga caggatcgag taggcgcggg cggtctcggg gttctcgtcg atgttcagtt
     1501 tgacgaccct caccttgtca gcgtgctcgt tcgcgatctc ttcgagcaca ggcgccacca
     1561 tgcggcaggg accgcaccat tcggcccaga agtcgaccag caccggcgta tcgctagcga
     1621 ggacgtcggt ggcaaacgtc tcgtcggtga cggacttggt ggctcccacg atgggctcct
     1681 gtcggttgat gtgattcgta cgggtttgta gcatttgggg tactactcgg cgaaggccgg
     1741 tttcaatgcg gcctgctggg cagccagcca acgctcggca tcgagcgcgg ccgcgcagcc
     1801 ggtgcccgct gcggtgatcg cctgacggta ggtgtggtcg accagatccc cacaggcgaa
     1861 gaccccatcg aggttggttc gcgtcgaggg ctgttggact ttgacgtaac cagcagcgtc
     1921 gaggtcgacc tgaccacgga agagttcgct gcgcgggtcg tggccgatgg caacgaacag
     1981 gcccgttacc ggcagctgat cgtcggcgcc cgtgaccgtg tcgcgcacgg tcagcccggc
     2041 gaccttgtcg tcgccgatca cgtcgaccac ctcggtgttc cagcggaagc cgaccttgtc
     2101 gttggccttc gcacgatctt gcatgatctt gctcgcgcga agctcgtcgc ggcgatggac
     2161 cacggtgacg ctgcgcgcga acttcgacag gaacgtggct tcctcgagcg cggagtcacc
     2221 gccgccaacg acggcgatgt cttggtcgcg gaag
//
LOCUS       DASWVF010000707         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_19780, whole genome shotgun sequence.
ACCESSION   DASWVF010000707 DASWVF010000000
VERSION     DASWVF010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19780"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..295)
                     /locus_tag="VGD55_12430"
     tRNA            complement(225..295)
                     /locus_tag="VGD55_12430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Gly,seq:ccc)
     gene            503..1033
                     /locus_tag="VGD55_12435"
     CDS             503..1033
                     /locus_tag="VGD55_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0871199.1"
                     /translation="MATEPILIPDATAEPAAYKQALLDLIGETDPVETMAATVAQWRE
                     ITSALTPEQLTTEPVDGEWSAAEITGHLFDVDLVYGFRWRLLLTEDSPTYPGYDEKLW
                     TPMPRPPFPALLDAWAGLRAANMLVLQANWPAAAQRVAIHSQQGPETFDVTLRKIAAH
                     DLAHLNQLRRTAEAAV"
     gene            1077..1652
                     /gene="dcd"
                     /locus_tag="VGD55_12440"
     CDS             1077..1652
                     /gene="dcd"
                     /locus_tag="VGD55_12440"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY0871200.1"
                     /translation="MLLSDRDIRAELAAGRVILEPYDPIMVQPSSVDVRIDKYFRVFE
                     NHRYPHIDPAIEQPDLTRQVEPDVDEPFILHPGEFVLASTYEVITLPDDVAGRLEGKS
                     SLGRLGLLTHSTAGWIDPGFSGHVTLELSNVATLPIKLWPGMKIGQLCLFRTSSPSEF
                     PYGSSVYGSRYQGQRGPTPSRSWQNFHRTQI"
     gene            1673..2032
                     /locus_tag="VGD55_12445"
     CDS             1673..2032
                     /locus_tag="VGD55_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0871201.1"
                     /translation="MHARISEIVLDCPDPDVMADFYSALTGWPVVYREEDWISLKGDA
                     GVKLAFQLAPDHVAPQWPNPGHPQQLHLDFIVDDADEAQQWAIGLGARVLDASPEHKT
                     FRVYSDPAGHPFCLCTS"
     gene            complement(2029..>2474)
                     /locus_tag="VGD55_12450"
     CDS             complement(2029..>2474)
                     /locus_tag="VGD55_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0871202.1"
                     /translation="TQLLASLVAEGRLHAASAIDSSVTYHDPCYLGRHNKVYGEPRDV
                     LAAVPGLTSTEMPRCKERGFCCGAGGARMWMEERVGKRINHERIDEALALDPDLVVTA
                     CPYCRVMLGDAVRDRQSSGEARGDVEVIDVAEVLLRSVRGPGPAS"
BASE COUNT          455 a          863 c          735 g          421 t
ORIGIN      
        1 gatacaccct cattgcagtc atccccttcg cattttgtgg tcgggaacca agacggccca
       61 cgacatcgca aatgttgaaa ggtctgccga aggctggtcg gatacttaca tgcatcccga
      121 gggcagcgga agagccaatc cgggtgtatc cggcttccct tatgcggggg cctgttagtg
      181 gatcttctgg acgaggtgcc gcaggtcgtt gtctggagaa agtgagcggg tgacgggaat
      241 cgaacccgca ctgtcagctt gggaagctga tgttctgcca ttgaactaca cccgcacgtg
      301 cgccccagcc acggccgaaa ccagcggtcc gagcgaacga cccgcctact ctaccgacgt
      361 cccggcccac cgtcggtgtc gaagtatcca tcgtcgtgca ggcccaccta catcgacgat
      421 gaagccgtag ctaatgcagt tggcgcaaca cacacgcacc gggtaaacag tcggtcacca
      481 gccccggcgt gcggaggaaa ccatggcgac tgaaccgatt ctgatccccg acgcgacggc
      541 cgagccggcc gcatacaagc aggcgctgct cgatctcatt ggcgagaccg atcccgtcga
      601 gacgatggcc gccacggtcg cacaatggcg cgagatcacg tccgcgctga cgcctgaaca
      661 actgacgaca gaaccggtcg acggcgagtg gtcagccgcc gagatcaccg gacacctctt
      721 cgacgtcgac ctggtctacg gctttcgttg gcgactgctg ctcaccgagg actccccgac
      781 ctatcccggc tacgacgaga agctgtggac cccgatgccg cgcccaccat tcccggccct
      841 gctcgatgcc tgggccggcc tgcgggcagc caacatgctc gtgctccagg cgaactggcc
      901 cgccgcggca cagcgcgtgg cgattcactc ccagcagggc ccggagacgt tcgacgtgac
      961 gcttcgcaag atcgccgcgc acgatcttgc ccatctcaac cagcttcgcc ggacggcgga
     1021 agccgccgtc tgaacgcgac cgaacgtcag ggcagaagga ccggtagatt gcccgcgtgc
     1081 tgctctccga ccgcgacatc agagccgagc tcgcggcggg ccgcgtgatt ctcgaaccgt
     1141 acgacccgat catggtgcag ccgtccagtg tcgacgtccg catcgataag tacttccggg
     1201 tcttcgagaa ccaccgttat ccccacatcg accccgcgat cgagcagccc gacctcaccc
     1261 gccaggtcga gcctgacgtc gacgagccgt tcatcttgca ccccggtgag ttcgtgctcg
     1321 cctcgacgta tgaggtcatt accttgccgg acgacgtcgc tgggcgcctc gaggggaagt
     1381 cgagcctcgg tcgcctgggg ctgttgaccc acagcaccgc cgggtggatc gatcccggat
     1441 tctctggcca cgtgactctc gagctgagca acgtcgcgac gttgccgatc aagctctggc
     1501 ccggcatgaa gatcggccag ctctgcctct tccgcacgtc ctcgccgtcc gagttcccgt
     1561 acggcagttc tgtctatggg tcgcgatatc aaggacaacg cgggccgacg ccgtcccggt
     1621 cgtggcagaa ctttcaccgc actcagatct gagccttgac cgaggagttc acatgcacgc
     1681 gcggatcagc gaaatcgttc ttgattgccc cgacccagat gtcatggccg acttctactc
     1741 agccctgacc gggtggccgg tcgtgtaccg agaggaggac tggatctccc tcaagggcga
     1801 tgccggcgtg aagctcgcgt tccaattggc gcccgaccat gtcgcgccgc agtggccaaa
     1861 cccgggtcat cctcagcagc tccacctcga cttcatcgtc gacgacgctg acgaggccca
     1921 gcagtgggcg atcgggctcg gcgcgcgcgt gctcgacgca tcgcccgagc acaaaacctt
     1981 ccgcgtctac tccgaccctg ccggccaccc gttctgcctc tgcaccagct agctcgctgg
     2041 gcccgggccg cgtaccgacc gcaacaacac ctcagccacg tcgatcacct cgacgtcgcc
     2101 gcgggcctcc cctgacgact gccgatcacg caccgcatcg ccgagcatga cccggcagta
     2161 cgggcacgcg gtcaccacga ggtccgggtc gagcgcaagc gcttcgtcga tgcgttcgtg
     2221 gttgatgcgc ttgccgacgc gctcctccat ccacatgcgt gcaccgcccg cgccgcaaca
     2281 gaatccgcgt tccttgcagc gcggcatctc ggtcgacgtc agcccaggca cggcggcgag
     2341 cacatcgcgc ggttcgccgt acaccttgtt gtgacggccg aggtagcacg gatcgtggta
     2401 ggtcactgac gaatcaatgg cggacgccgc gtgcaaccga ccttcggcta ccagcgaagc
     2461 caacaactgg gtgt
//
LOCUS       DASWVF010000708         5596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_427334, whole genome shotgun sequence.
ACCESSION   DASWVF010000708 DASWVF010000000
VERSION     DASWVF010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427334"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..248
                     /locus_tag="VGD55_12455"
     CDS             15..248
                     /locus_tag="VGD55_12455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY0871203.1"
                     /translation="MKTAGALVGGGLALAGGAVGAAIGDGIAGNALISGVARQPEAQS
                     RLTTLFFITVGLVEAAYFINLVFMILFVYVLGK"
     gene            259..834
                     /locus_tag="VGD55_12460"
     CDS             259..834
                     /locus_tag="VGD55_12460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0871204.1"
                     /translation="MPTVTASTVTASSNFLIPNGTFIAELVGFVIIVVVLARTLVPRI
                     NKALAERQEMIRRSYSDAEEAQARLDAAEAEYRELIAAARADAAKQREEAREEGAQIL
                     ADLRAQAQVESERLRQAAQQQLEAERSRTVEALRAEVGALAVELAGRVVGEALADDAR
                     QRRVVERFLTDVESRAGEAAGSAAAGEQVRG"
     gene            834..1676
                     /locus_tag="VGD55_12465"
     CDS             834..1676
                     /locus_tag="VGD55_12465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0871205.1"
                     /translation="MQGASRDALAHAWTYVERQLTRSGDEHHEASAVGEELFGVVAVL
                     DEQVGLRRALSDPAVDGKRKADLAKAVFGSHVSDITLRIVTEVVGARWSRMRDLADAT
                     ETLAVLAMLTEADAHPGSGHRAGESDDVEDELFRFGRIVEANAALRAALADPALPDEN
                     KVSLVNALVEDKARPVTVQLLTQIATRPRGRSPEDAIADYTNIASRRRARLVARVTTA
                     VALSDTEIARLQEALAGLYGHEVHLQIEVDADIVGGVVVQVGDEVLDGSVAGRLAEAR
                     RRLE"
     gene            1749..3398
                     /gene="atpA"
                     /locus_tag="VGD55_12470"
     CDS             1749..3398
                     /gene="atpA"
                     /locus_tag="VGD55_12470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0871206.1"
                     /translation="MAELTIRPEEIRDALERFVSSYTPDVASREEIGRVTEAGDGIAR
                     IEGLPSTMANELLRFADGTLGIALNLDVREIGAVVLGPFEGIEEGQIVKRTGEILSVP
                     VGDGFLGRVVNPLGEPIDGLGEIEAEGRRALEIQAPTVVQRQPVKQPLQTGIKAIDAM
                     TAIGRGQRQLIIGDRQTGKTAVAIDTIINQKENWATGDPKQQVKCIYVAIGQKGSTIR
                     AIQGALEDSGAMAYTTIVAAPASDAAGYKYLAPYTGSAIGQHWMYKGEHVLIVFDDLS
                     KQAEAYRTVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSMTGLPIIETK
                     GNDVSAYIPTNVISITDGQIFLETDLFNSGVRPAINVGISVSRVGGSAQVKAMRSVAG
                     RLRLDLAQFRELEAFAAFGSDLDAASRAQLERGSRLVELLKQAQYSPFPVEQEVVSIW
                     AGTTGQLDDVPVADIRRFEKEFLEHLRLQNKGILDSIAQTKELSDETAESLKGAITAF
                     KKNFETSEGKSLVGNEEAEDLDESTMSNEKVRKYVPPPADK"
     gene            3407..4309
                     /locus_tag="VGD55_12475"
     CDS             3407..4309
                     /locus_tag="VGD55_12475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY0871207.1"
                     /translation="MGAQVRVYRRRIRSVRATAKITRAFELIAASRMIKAQQRVAAST
                     PYADAITRAVSAVASQSNIDHPLTTKHENNTRAAVLLVTSDRGLAGAYSSNVLKEGES
                     LTAFLRESGFEVLPYIVGRKGLAYYRFRDRVIAGEWSGFSESPSYANAKDIADTLIAA
                     FNTPTEDGGVDEIHVVYTQFISTLTQTPIIRRVLPLEVEETTEELAGGPLPPYEFEPS
                     AEGVLDALLPRYVEARVYNALLQGAASELAARRRACKSATDNAEELIKTYTRLANAAR
                     QADITQEISEIVGGADALAATGSE"
     gene            4316..>5596
                     /gene="atpD"
                     /locus_tag="VGD55_12480"
     CDS             4316..>5596
                     /gene="atpD"
                     /locus_tag="VGD55_12480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0871208.1"
                     /translation="MTATVDKAAGSDAGHTGIGRVARVIGPVVDVEFGVDDLPEIYNA
                     LEVDIDLSGEDADVEGLHKHTLTLEVSQHIGDNLIRAISMQPTDGLVRGAPVRDTGSQ
                     ITVPVGDVTKGHVFNALGKVLDVPEESLKITERWPIHRSSPPFDQLESKTEMFETGIK
                     VIDLLTPYVLGGKIGLFGGAGVGKTVLIQEMITRVAKNFGGVSVFAGVGERTREGNDL
                     FLEMTESGVIKDTALVFGQMDEPPGTRLRVALAALTMAEYFRDVQNQDVLLFIDNIFR
                     FTQAGSEVSTLLGRMPSAVGYQPTLADEMGVLQERITSTRGHSITSLQAIYVPADDIT
                     DPAPHTTFAHLDATTVLSRPISEKGIYPAVDPLDSTSRILDPQYIGEEHFRVANRVKQ
                     ILQRYKDLQDIIAILGIDELGEEDKVLVGRARRIE"
BASE COUNT         1006 a         1835 c         1830 g          925 t
ORIGIN      
        1 atcttgacag cgcgatcaag actgctggcg cgctggtcgg tggcggactc gcgctcgccg
       61 gcggcgcggt aggagccgcg attggtgacg gcatcgccgg taacgcactc atctccggtg
      121 tcgcccgcca gcccgaggcg cagagccgcc tcaccacgct gttcttcatc acggtcggtc
      181 tggtcgaggc tgcctacttc atcaacctcg tcttcatgat ccttttcgtg tacgtcctcg
      241 ggaagtagga agtcgaccgt gccgactgtc accgcgtcga ccgtcactgc gtcgtcgaat
      301 ttcctgatcc cgaacggcac gttcatcgcc gagctggtgg gctttgtgat catcgtcgtc
      361 gtgctggcca ggacgcttgt gccgcgcatc aacaaggccc tcgctgagcg ccaggaaatg
      421 attcgtcgca gctacagcga tgccgaggaa gcgcaggcgc gtcttgacgc tgccgaggcg
      481 gagtaccgcg agctgattgc cgccgcgcgt gctgacgccg cgaagcagcg cgaggaggct
      541 cgcgaggaag gcgcccagat tctggccgac ctgcgtgcgc aggcccaggt cgaatccgag
      601 cgcctcaggc aggccgcgca gcagcaactc gaggccgaac gctcgcggac ggttgaggca
      661 ttgcgtgccg aagtcggcgc gcttgccgtc gagctcgcgg gtcgggtcgt cggcgaggcg
      721 ctggccgatg acgctcgtca gcggcgggtc gtcgaacggt tcctcaccga tgtcgagtcg
      781 cgcgcgggcg aagcagcagg atcggcagcc gccggtgagc aggtgcgcgg ctgatgcagg
      841 gagctagtcg ggacgcactt gcgcacgcgt ggacctacgt cgaacgccag ttgacacgaa
      901 gcggcgacga gcaccacgag gcgtctgctg tcggcgagga gctgttcggc gtcgtggccg
      961 tcctcgatga gcaagtgggc ctgcgccgcg cgctgtctga ccccgccgtc gacggcaagc
     1021 gtaaggccga cctggcgaag gcggtgttcg gctcgcatgt gtcggacatc acgctgcgga
     1081 tcgtgaccga ggtcgtcggc gctcggtggt cacgcatgcg tgaccttgcc gacgccaccg
     1141 agacgcttgc ggtgctagcg atgttgaccg aggccgacgc tcaccccggc agcggccacc
     1201 gcgcaggcga gtccgacgac gtcgaggacg agctgtttcg gtttggccga atcgtcgagg
     1261 caaacgctgc cctgcgcgca gcgctcgccg atccggcatt gcccgacgag aacaaggtgt
     1321 cgttggtcaa cgcactcgtc gaggacaagg cgcggccggt caccgtgcag ctgttgacgc
     1381 agatcgcgac gcgtccccgt ggtcgctcgc ccgaggatgc gattgccgac tacaccaaca
     1441 ttgcgtcgcg tcgtcgtgca cggctggtcg cgcgtgtaac cacggcagtc gcgctctccg
     1501 acaccgagat cgcccgcttg caggaggccc tcgccggcct ttacggccac gaggtgcatc
     1561 ttcagatcga agtcgacgcc gacatcgtcg gcggtgtggt cgtgcaggtc ggtgacgaag
     1621 ttctcgacgg ctcagtcgcg ggtcggctgg ctgaggcaag gcgacgtctt gaataacaac
     1681 ccgcgcgcat caatgccagg gacgtccgca ccacgagtct tacgaaaccc acgaacgagg
     1741 atggactgat ggcagagctc acgatccgcc cggaggagat ccgcgacgcg ctcgagcgtt
     1801 tcgtgtcgtc gtacacccct gacgtcgcat cgcgcgaaga gatcggccgg gtgaccgaag
     1861 ctggcgacgg catcgcgcgc atcgaggggc tgccttcgac gatggctaac gagttgctcc
     1921 ggttcgccga cggcacgcta ggcatcgccc tgaacctcga cgtgcgcgag atcggcgcgg
     1981 tcgtcctcgg tccgttcgag ggcatcgaag aaggccagat cgtcaagcgc accggcgaga
     2041 tcctgtccgt gccggtgggt gacggctttc tcggccgggt cgtcaacccc ctcggcgagc
     2101 cgatcgacgg cctgggcgag atcgaggccg aaggtcgtcg ggcactggag attcaggcgc
     2161 cgacggttgt tcagcgccag ccggtaaagc agcccttgca gaccggcatc aaggccatcg
     2221 acgcgatgac ggcgatcggg cgcggtcagc ggcagctcat catcggcgac cggcagaccg
     2281 gcaagaccgc ggtggcgatc gacacgatca tcaaccaaaa agagaactgg gcgaccggtg
     2341 atcccaagca gcaggtgaag tgcatctacg tcgcgatcgg gcagaagggc tcgacgatcc
     2401 gagcgatcca gggcgcactc gaagactccg gcgcaatggc gtacaccacg atcgttgcgg
     2461 caccggcgtc tgacgcggcc ggctacaagt acctcgcgcc gtacaccggc tcggcgatcg
     2521 gccagcactg gatgtacaaa ggcgagcacg tgctgatcgt cttcgacgac ctctccaagc
     2581 aggccgaggc ctaccgcacg gtgtcgctgc tgctccgccg tccgcctggt cgtgaggcct
     2641 accccggcga cgtcttctac ttgcactcgc ggttgctcga gcgttgcgcg aagctgtccg
     2701 acgaactcgg cggcggatcg atgaccgggt tgccgatcat cgagaccaag ggcaacgacg
     2761 tctcggccta catcccgacc aacgtgatct cgatcaccga cggccagatc ttcctcgaga
     2821 ccgacctctt caactcgggc gtgcgaccgg ccatcaacgt cggtatctcg gtgtcgcgag
     2881 tcggcgggtc ggcacaggtc aaggcgatgc gttcggttgc cggtcgcttg cgactcgacc
     2941 tagcgcagtt ccgcgagctc gaggcgttcg ctgctttcgg ctccgatctc gacgccgcat
     3001 cgcgtgcgca actcgagcgt ggttcgcggc tcgtcgagct gctgaagcag gcgcagtact
     3061 caccgttccc cgtcgagcaa gaagtcgtgt caatctgggc cggcacgacg ggtcagctcg
     3121 acgacgtgcc ggtggccgac atccgccggt tcgagaagga gttcctcgag cacctgcgcc
     3181 tgcagaacaa gggcatcctc gactccattg cgcagactaa ggagctctcc gacgagaccg
     3241 ccgagagcct gaagggcgcg atcacggcct tcaagaagaa cttcgagacc tccgaaggca
     3301 agtcgctcgt tggcaacgaa gaagccgaag atcttgacga gtcgacgatg tcgaacgaga
     3361 aggtccgcaa gtacgtcccg ccgccggctg acaagtagat cgcgctatgg gtgctcaggt
     3421 ccgggtctat cgccgccgca ttaggtcagt gcgcgccacg gcgaagatca cccgtgcctt
     3481 cgaactgatc gcggcgtcgc gcatgatcaa ggcgcagcaa cgggtggctg cctccacgcc
     3541 gtatgccgac gcgatcactc gcgccgtctc ggcggtcgcg agtcagtcaa acatcgacca
     3601 cccgctgacg acgaagcacg agaacaacac gcgggccgct gtcctgctcg tcactagtga
     3661 ccgcggtctc gccggtgcgt acagctcgaa cgtgctgaag gagggcgagt cgctcacggc
     3721 gttcttgcgt gagagtggct ttgaggtgtt gccctacatc gtgggccgca agggcttggc
     3781 gtactaccgc ttccgcgatc gcgtcatcgc gggggagtgg agcggcttct ccgagagccc
     3841 gagctacgcc aacgccaagg acatcgccga cacgttgatc gccgcgttca acaccccgac
     3901 cgaagacggc ggcgtcgacg agatccacgt cgtctacacg cagttcatct cgacgctgac
     3961 gcagacaccg atcatccggc gggtcctgcc cctcgaggtc gaagagacca ccgaagaact
     4021 ggctggcggc ccgctgccgc cgtacgaatt tgaaccgagt gccgaagggg tgctcgatgc
     4081 gctgcttccg cgctacgtcg aggcgcgcgt ctacaacgca ttgcttcagg gcgctgcatc
     4141 agagctggcc gctcgtcgtc gcgcgtgcaa gtcggcgacc gacaacgcgg aagagctcat
     4201 caagacctac acgcggttgg cgaacgcagc tcgccaggcc gacatcaccc aagaaatcag
     4261 tgagatcgtc ggtggcgctg acgcgctcgc cgctaccggg agcgagtgag aactcatgac
     4321 tgcaacagtc gacaaggccg caggttctga cgccgggcac accggcatcg gccgcgttgc
     4381 ccgtgtcatc ggcccggtgg tcgacgtcga gttcggcgtc gacgacctgc cggagatcta
     4441 caacgcactc gaggtcgaca tcgacctttc aggcgaggac gcggacgtcg aggggttgca
     4501 caagcacacg ctgaccctcg aggtctcgca gcacatcggc gacaacctca tccgcgcgat
     4561 ctccatgcag cccaccgacg gactcgtgcg tggtgcaccg gttcgtgaca ctggcagcca
     4621 gatcaccgtg cccgtcggtg acgtgaccaa gggccacgtc ttcaacgccc ttggcaaggt
     4681 gctcgacgtc cccgaagagt cgctgaagat caccgagcgc tggccgatcc accgttcgtc
     4741 gccgccgttc gaccagctcg agagcaagac cgagatgttc gagaccggca tcaaggtgat
     4801 cgacctgctc accccctacg tgctcggcgg caagatcggc ctgttcggcg gtgccggtgt
     4861 cggcaagacc gtgctgatcc aggagatgat cacccgcgtc gccaagaact tcggcggtgt
     4921 gtcggtgttc gccggtgtcg gtgagcgcac ccgtgagggc aacgacctct tcctggaaat
     4981 gaccgaatcg ggcgtcatca aggacaccgc gctcgtgttc ggtcagatgg acgagccgcc
     5041 gggcacccgt cttcgggtcg cgctcgccgc gctcacgatg gcggagtact tccgcgacgt
     5101 gcagaaccag gacgtgctgc tcttcatcga caacatcttc cggttcacgc aggccggctc
     5161 tgaggtgtcg acgttgctcg gccgcatgcc gtcggcagtc ggttaccagc cgacgctggc
     5221 cgacgagatg ggcgtgctcc aagagcgcat cacgtcgacc cgcggtcact cgatcacctc
     5281 gctgcaggcg atctacgtgc ccgccgacga catcaccgac cctgcgccgc acaccacgtt
     5341 cgcgcacctc gacgcaacga ccgtgctgtc gcgtccgatt tcggagaagg gcatctaccc
     5401 cgcggtcgac ccgctcgact cgacgtcgcg cattctcgac ccgcagtaca tcggcgagga
     5461 gcacttccgc gtcgcgaacc gggtgaagca gatcctccag cgctacaagg acctgcagga
     5521 catcatcgcg atcctcggca tcgacgaact cggtgaagaa gacaaggttc tcgtcggtcg
     5581 cgcccgtcgc atcgag
//
LOCUS       DASWVF010000709         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_427342, whole genome shotgun sequence.
ACCESSION   DASWVF010000709 DASWVF010000000
VERSION     DASWVF010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427342"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VGD55_12485"
     CDS             complement(<1..1443)
                     /locus_tag="VGD55_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0871209.1"
                     /translation="MTTGVVVILAIAAALVGLAAEYARRLQTAQTALLEALRPVANGD
                     LTTKINISAGRRGAEVADFVTAIADRLARVLTQVEHTKSQLTAGWHDVDEMAWKSLDN
                     TETTAARVASAARSAEDISEHLHLIATATEELAATIQEIARHASEASGVAATATSEVG
                     AANHTVGELAAASRHIDEVLHLIAMIATQTHMLSLNATIEAARAGAAGSGFAVVAGEV
                     KKLSQQTGEATTSVSASVASIQDGVGLAAEAMGRVTATITRVNDNQQGIAAAVDQQSL
                     TTRDIGANATEAARGATALADDVSALVASIRLAAYAGAHARTVAAELADLDSGLGSML
                     DLYKFNRVQLDLATEVAESVGVRRVGNVTTIPHYVTGSGLNEIEFTENWRQSKANLES
                     DSGDAYCGMPGDVATVRFAGTRIRYYGAPESNRGMIAISVDGGPETIVDQYGLTRDTK
                     MFWQSDVLLAGQHTMRVRVTGEQNPDSRYIW"
BASE COUNT          291 a          646 c          645 g          368 t
ORIGIN      
        1 ccagatgtag cgcgagtcgg ggttctgctc gccggtgacg cgtacgcgca tcgtgtgctg
       61 accggccagg agaacgtcgg actgccagaa catcttcgtg tcgcgggtga gtccgtactg
      121 atcgacgatc gtttcaggcc cgccgtcgac cgagatggcg atcatgccgc gattcgactc
      181 gggagcaccg tagtagcgga tcctggtacc cgcgaatcgc acggtggcga cgtcgcccgg
      241 catgccgcag taagcgtcgc cgctgtcgct ttcgaggttg gccttcgact gtcgccagtt
      301 ctcggtgaac tcgatctcat tcagccctga ccccgtgacg tagtgcggga tcgtcgtcac
      361 gttgccgacg cggcgaaccc caacactttc ggctacctct gtggcgaggt cgagttgcac
      421 ccggttgaac ttgtacaagt cgagcatcga accgagcccg gagtcgaggt cggccagctc
      481 ggcggcgaca gttctcgcat gtgcgccggc atacgccgca agccgaatac ttgccactag
      541 cgcgctgaca tcgtcggcga gtgcagtggc cccacgggcc gcttccgtcg cgttcgcgcc
      601 gatgtctctg gtggtcaggc tttgctggtc aacggccgcc gcgatgccct gctggttgtc
      661 gttgacgcgc gtgatggtgg ccgttacccg gcccattgcc tcggcggcca accccacgcc
      721 gtcctggatc gatgcgaccg aagcgctgac actcgtcgtc gcctcgcctg tctgttgcga
      781 caacttcttg acctcgccag cgaccaccgc gaagccgctg cccgcggcac ctgcacgggc
      841 cgcctcgatg gtcgcgttca acgacagcat gtgggtctgc gtggcgatca tcgcgatcag
      901 gtgaagcacc tcgtcgatgt ggcgagacgc ggccgccaat tcgccgaccg tgtggttggc
      961 cgcaccgacc tctgacgtcg ccgtggcggc gaccccggac gcttcggaag cgtgccgggc
     1021 gatctcctgg atcgtcgccg cgagttcttc ggtcgccgtt gcgatcaggt gcaggtgttc
     1081 tgagatgtcc tctgcggagc gagcggctga cgccacccgt gcggcggtcg tctcggtgtt
     1141 gtcgaggctc ttccaggcca tctcgtcgac gtcatgccag cccgcggtga gttgtgactt
     1201 ggtgtgctcg acctgggtca gcacccgggc cagtcggtcg gcgatcgcgg tcacgaaatc
     1261 agcgacctca gcccctcggc gtccggccga gatgttgatc ttcgtggtga ggtcgccgtt
     1321 cgcgaccggg cgcagcgcct cgagcagtgc tgtctgcgcg gtctgtaggc gccgcgcgta
     1381 ctccgcagcg aggccgacga gggctgctgc gatagcgagg atcaccacga cgcccgtcgt
     1441 catgccacct ccgccacctc aggctcgctg tccctgaaga catcgacgcc gggtgagctg
     1501 ccttgaggtc ggcggcagct ggtgagtgga ggcgctcgac gaaatccggt catgccccct
     1561 cgtactagtg caaggttggg cggaccgggc gaattccgtg ctccaatttg cgcgaaactt
     1621 ggccggaacg acccgagtcg gatctgttct tgtttggttt cgggcgcgcc gcgtgcatga
     1681 gcgaaaaccg atcaggcact ctgaatccgc gcgacgacga cgggtcactt gtccccccgg
     1741 gtgacacggg cttgtcgcga agtggcggtc cggctcgcgc atcccccacg cagagccgga
     1801 ccgccgcgcc cccggatgca ggccgcaccc cccagctagc ggcctaccga gaaaaagtct
     1861 cgcaaacggc ggcatcggcg ggtattggcc gtccggccac aaccgtgagc gcatctgctc
     1921 atgttttgcg agcgttcggc gcagccgggt
//
LOCUS       DASWVF010000710        12062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_453290, whole genome shotgun sequence.
ACCESSION   DASWVF010000710 DASWVF010000000
VERSION     DASWVF010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453290"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGD55_12490"
     CDS             <1..363
                     /locus_tag="VGD55_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0871210.1"
                     /translation="GLGVIPWSPLQGGLLGGILRKQDSNRSKSGRADDTLAANRPAVE
                     AYEAFCDELGEEPANVALAWLLHQPAVTAPIIGPRTIDQLNGAIRAIEIKLDDKALAR
                     LDEIWPGPGGEAPEAYSW"
     gene            complement(452..946)
                     /locus_tag="VGD55_12495"
     CDS             complement(452..946)
                     /locus_tag="VGD55_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0871211.1"
                     /translation="MDLTDRQIIAELEADGRLTVTELAERVNLTVSPCHRRLRELEQS
                     GVIRGYRAVIDPAAVGLGFEVLVHVTMDREDAATIAMFESGLAAVGEVRHAERLFGDP
                     DYLLRIATADIAAYQSLRDEKLATLPGVQRLTSTIVMKRIVNDRPFPIAESAAATDPH
                     QVRR"
     gene            1085..1672
                     /locus_tag="VGD55_12500"
     CDS             1085..1672
                     /locus_tag="VGD55_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871212.1"
                     /translation="MGDVLDYAPIAATAFAIPQFLPQLVKLRKTGDTAGVSWPWAVLT
                     SVNNAAWAAYFVLFRYWRALVPACAATLLAGVLAAMLAARGRAKLRPALLTVGWAALL
                     TAACVAGRSVLGTLLTAGFVLQITPPIWVAYRTDRPTGISYGSWLLIVAELACWAAFG
                     VHESDPRLIVLGCTGVTAGILMLLRIRHTAVARCS"
     gene            1712..2551
                     /locus_tag="VGD55_12505"
     CDS             1712..2551
                     /locus_tag="VGD55_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0871213.1"
                     /translation="MTHPESPTLLLNDGRQLPQLGLGTYGLNGPRGTDAVVAAADLGY
                     RLFDTAFRYGNEAAVGAGINTASATRNELFVTTKFDGEFQGGGRALRGLDDSLRRMGL
                     DYVDLILIHWPLPRRDLYVDTWNTFEQLLASGKTRSIGVSNFKPAHLERLRKDTAVVP
                     AVNQIELDPTIARVDARAYHDAHGIVTQAWSPLGGAGSPISRNPTIAEIAAAHGRTPA
                     QIALRWCIEIGVSTVPKTGNVARMVENIDIFDFALTGDDMARIAALDEGENAAQDSDR
                     IGH"
     gene            complement(2588..3892)
                     /locus_tag="VGD55_12510"
     CDS             complement(2588..3892)
                     /locus_tag="VGD55_12510"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0871214.1"
                     /translation="MFRALSVRNYRLYAGGQVVSNTGTWMARVTQDWLVYHILTHDNS
                     FALGVVTALQFMPALAFGMYGGMLGDRYPKRVVLTITQSAMALLSLLSGALIVAHAME
                     LWTICLIALVFGVASALDMPVRQAFVIEMVGRDVLQNAVSLNSATFNAARMVGPAVAG
                     LLIEAFGAAPGFFFNAASYLAVIIGMLAMTKTELHVAPRVARARGQLREGLTYVRNRP
                     ELLLPVMLMGFIGTFGFNFQITNALIAQGVFHRGAGSYGILSTALAVGSLGGALLGAR
                     RRQRPRVRLLIIAGLVFSVLVAVSGFVPSYPLFMLLLIPTGGAGILLATSCNAVLQLS
                     AAPEMQGRVMALYTTVFVGCAPFGSLLVGWLGGAFNPRWALLAGGLICAVSVVGCALF
                     YVRAQRLEIRVADHVWWPRALAQDQFRDELEDEALLADVQAR"
     gene            complement(3898..4329)
                     /locus_tag="VGD55_12515"
     CDS             complement(3898..4329)
                     /locus_tag="VGD55_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0871215.1"
                     /translation="MPSKTATSELASELRVSLMRLARRLRAERLDEGLTPTQLAVLGS
                     LDRHGPMTLGELAAHEKVQPPSMTRTTAILEERGLLTRVTEEVDRRQVRIAISDAGLA
                     LLREDRKRRDAWLAARFDQLTGDERALLHQVAPLLDRLAQS"
     gene            4401..4706
                     /locus_tag="VGD55_12520"
     CDS             4401..4706
                     /locus_tag="VGD55_12520"
                     /inference="COORDINATES: protein motif:HMM:NF022205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HEY0871216.1"
                     /translation="MAAPRRPDPPPLQTNEFAPVIVATGLWVVAFFILLSQHHSLASH
                     GHGWYVWVGLSGFLLGLWGLSLMLLRRRGIQRRAQSRSASASSTDDGTAPRSNSLST"
     gene            complement(4634..7498)
                     /locus_tag="VGD55_12525"
     CDS             complement(4634..7498)
                     /locus_tag="VGD55_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871217.1"
                     /translation="MLASWAASPARFREDANAEEDLVLGGYRDRVLIELAQNAADAAG
                     RAGVRGRLRFDLDSADAVLRASNTGAPIDAAGVESASTLRASSKRDQGSSGAVGRFGV
                     GFAAVLAVTDEPSIVATEGSVTWSLDRLRDDVAAIPALAAEVARRGTALPILRLPYAG
                     EGLRPGDGFDTEVVLPLRDATAVELVRSLLDDVDAVLLLTLPTLATVEIVIDGAAREL
                     HAVETADGLVVDGTRWIVHETNGRFDAELLRDRPVEERGRRDFVLTWAVPVDEAGAPV
                     ALPASVPGVVHAPTPTDEPLTLPALLVAPLPLDPTRRHVAPGPLRDALLDHAATAYAD
                     LVRGMSASAALLDLVPVSVAAGAIDGELRARILPALREVPFLSTAESSIRMRPANAVM
                     LDDAGLASDDSLGVVLAPVLPELVPASWARRNPTALAALGVRRLPLGTLIDDLATVER
                     EPAWWHSLYSALAGAGISSDVLSGVPVPLGSGGVARSARGLLMPGQFGDLTVLGLRTI
                     HADAAHPLLLRLGAVEADPRAVLADDHVRAAVENSYDAAEPAEVADAILRLVAAANLQ
                     AGDEIWLAELALPADDGELFVAGELLMPQGKLAGVVADDAPFGTVDPALVERWGAQVL
                     EAVGVLDSFAIVTDTDVVGADHDLDLEGEYLDSVRAVLAVDEPVVVSEFVGVRDLEFV
                     RDDAWPAALALLSKPPLRATVVDRAVVIGGGLQARVPSYTAWWLRERRVVDGRLPASD
                     PLLHGLFDVVGIEGDDEFLEAAGALRDLDDADHDEIAARLGDPARTVTRDQTRALYAR
                     LEPRDAPALVRAVLRNELAVVRAGDAVVVDAPDLLGLLGEFAIVPASLDDATRVADAL
                     DVALASELGRFEVASTGVPHDDHVVHDPLLVVAVGGETQRVAWRLADGVLHVDAQSLD
                     FGLGRGRAWRDGQWSRRYVLSELLRGAVPSSVLLAEADLD"
     gene            complement(7534..8457)
                     /locus_tag="VGD55_12530"
     CDS             complement(7534..8457)
                     /locus_tag="VGD55_12530"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEY0871218.1"
                     /translation="MTQTPIDTTAPSVDKAEPVLAPDAVCAAAVDLARRAAQAVAGHQ
                     LVGDHAGVEADAERIVTHFFACTDRAYTAWRWAVTVVRAPRSKTVTVDEVVLLPGPDA
                     LLAPAWVPWSERLLAGDLGVGDVLPTAADDERLVPGYAAIGAHDETDDVDTIDALVNE
                     LWLGRPRVLSEFGRLEAAERWYEGDRGPEAPIARAADLHCASCGFLVLMRGALSRVFG
                     VCANEYAPDDGHVVSFDHGCGAHSEALVAQTEPLRTPEAIDLDIDEIGGDGGVAEVVD
                     VVTVDEVALDDDVPVDEVAEFALDDDAAGTH"
     gene            8514..9962
                     /locus_tag="VGD55_12535"
     CDS             8514..9962
                     /locus_tag="VGD55_12535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0871219.1"
                     /translation="MSEPRTTEPHQPDGFGRAVVHLIAGAGGGAARWFTRSTRAEGAD
                     ASGLASLIDLHAIQSAGDALLTVALANTLFFSVSVDQARSKVALYLLITMAPFAVIAP
                     VIGPLLDRFRNGRRYALAATLILRAFLAWVMAGAVGKHGGFALYPAAFGALVCSKAYG
                     VSRSAVVPRVLPPNVTLVRGNSRLTLWGVLTATVAAPIGQGLSWATGSPSWTLRLCAL
                     VYLAGSVFAFKLPERVDSSEGEVRLRRRPRRASQPRRSDDSQQREVARSTYRRILPPL
                     RGIGPRMTTMLRASAGLRAFTGFLTLFLAFLIRKHPLGVFVPGADLGIIVACAAIGSV
                     LGTTLGGWLKPRQPEILAIVSLFAASIIGIVTAIWFNLATAALAILIANVSQSLSKLG
                     LDSVIQRDAVEHVRTSAFARSETVLQLSWVLGGFVAILLPSNGSLGLALGAAVLVVVL
                     LSTMKAVRTRVSPDRGIELLRERRRPAVSSDT"
     gene            9959..10648
                     /gene="mqnB"
                     /locus_tag="VGD55_12540"
     CDS             9959..10648
                     /gene="mqnB"
                     /locus_tag="VGD55_12540"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HEY0871220.1"
                     /translation="MRVLVVCAVEAERDAVVRNFGAVRPVTVDGFSGVAVDTAAGELR
                     AFSVGVGPVAAAIGTSALIAGMDYDLVASAGIAGGFRGRVAIGDVVLANLATYADLGV
                     RVDDGFLSVRDLGIDQDSSLAFGDQRLIDRMSASGVLVGEVLTLSAMTGTDADAEELA
                     ARYPSALAEAMEGFAVVAATRDHGGIRYVAEIRAISNLIGRRDRSTWNIPLAFDALAD
                     AMSTLLNEPLP"
     gene            10645..11466
                     /locus_tag="VGD55_12545"
     CDS             10645..11466
                     /locus_tag="VGD55_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HEY0871221.1"
                     /translation="MTAGRLSLAFSPCPNDTFAFYAWTHGLIPDAPPVDITFADVDVT
                     NGAAERGELDVVKVSYAALPWLLDQYALLPSGGALGRGCGPLVLTAGRRDLRGASIVV
                     PGERTTAYLLFRLWAANQGVGEISVLPFPQIMPAVRDGRFDAGLVIHEARFTYTDYGL
                     TNVMDLGAWWEADTGLPIPLGAIIARRSLDTDVLARSARASVEHAFAHPSASASFVAA
                     HASEMDPDVQARHIALYVNEFSVDLGDEGYAAIDALLKRAADAGLVPPVDPAGLR"
     gene            11463..12044
                     /locus_tag="VGD55_12550"
     CDS             11463..12044
                     /locus_tag="VGD55_12550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0871222.1"
                     /translation="MSEQLALVQAGDQPRAVATLVSAFTDDPVERWLWPDDNAYATQF
                     PRFVAALGGRAFEVQTAWRLGDFAAVALWFAPGVEPDGELIVGVLSETVPQDKLDDTF
                     TVLEAMDSVHPQYPHWYLPWFGVTAAAQGRGLGSQLMTQCLEIIDASNLPAYLETPNP
                     RNISFYERHGFEVTGEVQAGNCPPVTFMLRPGH"
BASE COUNT         2080 a         4147 c         3898 g         1937 t
ORIGIN      
        1 ggcctcggcg tcataccgtg gagcccgctg cagggtggat tgctcggcgg tatcttgcgc
       61 aagcaagaca gcaaccgcag caagagtggt cgcgcggacg acacactcgc cgcaaaccgc
      121 cccgccgttg aagcctatga agcgttttgc gacgagctcg gcgaagaacc tgccaacgtc
      181 gcattggcgt ggctgctgca ccagccggca gtgaccgcac cgatcatcgg cccgcgcacg
      241 attgatcagc tgaacggcgc gatccgggcc atcgagatca agctcgacga caaggcgctc
      301 gcccgcctcg acgaaatctg gcccggcccg ggtggcgagg cgcccgaggc gtactcctgg
      361 tagtcgctgg cccaggcagc gctgtcgtcg gtgatcgcca ccgcgagggc agcgaaagca
      421 gccgcgatgg ccgaacggtc gctccttcgt tttaacgccg aacctggtgc gggtcggtcg
      481 cagctgccga ctcggcgatc ggaaacggcc ggtcgttgac gatgcgtttc atgacgatcg
      541 tcgaagtgag tcgttgcacg cccggcaggg tcgcgagctt ttcgtcgcgc aggctctggt
      601 aggcagcgat gtcagctgtg gcgatgcgca gcagatagtc ggggtcgccg aacagccgtt
      661 ctgcgtgccg gacctcgccg acggctgcca atccactctc gaacatcgca atcgtcgcag
      721 cgtcctcgcg gtccatcgtc acatggacga ggacctcgaa gccgagcccc accgcagcgg
      781 gatcgatgac agcgcgatat ccccgaatca ctcctgactg ttcaagttcg cgcaaccgcc
      841 ggtgacatgg cgagacggtc aagttgaccc gctcagccag ctccgtaacc gtcagacgcc
      901 catcggcttc aagctcagca atgatctgcc gatcagtgag gtccacaacg aggataattg
      961 cagcaaagtg tcgatctcag caagaacttg aacaaaaaga cctagccaag tgggatattt
     1021 tgccacctga aggcgatggc cgcctcatac aggcggtgct ggccctggag ggtcgcgaac
     1081 gatcatgggc gacgttctcg actatgcgcc aatcgccgca acggcattcg cgattccgca
     1141 gttcttgccg cagctggtca agctccgcaa gaccggcgac accgccggtg tctcatggcc
     1201 gtgggcggtg ctcacgagcg tgaacaatgc cgcatgggcc gcctacttcg tgctctttcg
     1261 atactggagg gcgttagtac ccgcctgcgc tgcgacgctg ctcgcggggg tgctcgccgc
     1321 catgctcgcc gcgcgcggcc gagccaagct gcggccagcg ttactcacgg tcgggtgggc
     1381 tgcgttgctg actgccgcct gcgtcgccgg gcgctcggtc ttgggcacgc tgttgacagc
     1441 gggcttcgtg ctccagatca cgccgccgat ttgggtcgcg tatcgaaccg atcgcccaac
     1501 aggaatctcg tacggatcct ggctgctcat cgtcgccgaa ctcgcgtgct gggcagcctt
     1561 cggcgtccat gagtcagacc cccgcctgat agttctcggc tgcactggag tgaccgccgg
     1621 aatcctcatg ctcctgcgga tccggcacac tgcagtcgca cgatgttcct gagcccaagg
     1681 ttcccgacct cacacagatt ggacagccaa cgtgacgcat ccggagtcgc cgacgctgct
     1741 cctgaacgac ggccggcagc tgccgcagct cgggctcggc acctacgggc tgaacggccc
     1801 tcggggcacc gatgcagttg tcgccgctgc tgacctcggc taccgattgt tcgacaccgc
     1861 gttcagatac gggaatgagg ccgcggtcgg cgcggggatc aacaccgcga gtgcgacccg
     1921 caacgagttg tttgtgacga ccaagtttga cggcgagttc caaggcggcg gtcgggcact
     1981 gcgcgggctt gacgactcgc tgcgtcgcat ggggttggac tatgtcgact tgatcctcat
     2041 ccattggcca cttccgcggc gagacctcta cgtcgacacc tggaacacgt tcgagcagct
     2101 actcgccagc gggaagacaa ggtcgatcgg tgtgtcgaac ttcaagcccg cacacctgga
     2161 gcggctgcgc aaagacaccg ccgttgtccc cgccgtcaat cagatcgagc tcgacccgac
     2221 gatcgcgaga gtcgacgccc gcgcgtacca cgacgcgcac ggcatcgtca cccaagcgtg
     2281 gagtccactc ggaggcgctg gttcgccgat cagcaggaac ccgacgatcg ccgaaatcgc
     2341 agcagcacac gggcgaacgc ccgctcaaat cgcgctgcgg tggtgcatcg agatcggcgt
     2401 ctcaacagtg ccgaagaccg gaaacgtcgc ccgtatggtg gagaacatcg acatcttcga
     2461 cttcgccctc accggcgacg atatggcccg catcgcggca ctcgacgaag gcgagaacgc
     2521 agcgcaggat tcggaccgca tcggtcactg atgaacacag cgctgatgaa ctaggcaccc
     2581 atcacgatca acgtgcttgc acgtcggcca gcaatgcctc gtcttcgagc tcgtccctga
     2641 actggtcctg tgcgagggct cgcggccacc acacatgatc ggcaacccgg atctccaggc
     2701 gctgcgctcg cacgtagaac agcgcgcacc cgacgacgga taccgcgcag atgagcccgc
     2761 ctgcaagtaa cgcccatcgc ggattgaacg cgccgccgag ccagccgacc aacaatgagc
     2821 cgaacggcgc gcagccaacg aacacggtcg tatagagcgc catgacccgg ccttgcatct
     2881 ccggcgcggc actcagctgc agcactgcat tgcacgacgt cgcaagaagg attccggcgc
     2941 caccggtcgg aatcagaagc agcatgaaca gcgggtagga aggcacgaag ccggacaccg
     3001 caacgaggac gctgaagacc agaccggcga tgattagcag ccgcaccctg ggtcgctgcc
     3061 ggcgacgggc gccaagtagc gcgccaccca gcgaacccac ggccagcgcg gtcgacagaa
     3121 tcccgtagct tcccgcacca cggtgaaaca cgccctgtgc gatgagcgcg ttcgtgatct
     3181 ggaagttgaa cccgaaggtc ccgatgaagc ccatcagcat gaccgggagc aacagttccg
     3241 gccgattacg cacgtatgtg aggccttcac gaagctgccc gcgggcgcgt gcgacacgag
     3301 gcgcaacgtg cagctcggtc ttggtcatgg cgagcatgcc gatgatgaca gccagataag
     3361 acgcggcgtt gaagaagaac cctggggcgg cgccgaaagc ctcgatcagc aagccagcga
     3421 ccgccggccc gaccatgcgt gccgcgttga aggtggccga gttgaggctc acggcgttct
     3481 gaagaacatc gcgcccgacc atctcgatga cgaatgcctg ccgcacgggc atgtcgagcg
     3541 ccgacgcgac gccaaacacc aaagcgatca aacagatcgt ccacagctcc attgcgtgcg
     3601 caacgatgag tgctccgctg agcagcgaca gcagcgccat cgcgctttgc gtgatcgtga
     3661 gcacaacgcg tttcggatag cggtcaccga gcatcccgcc gtacatgccg aaagccagcg
     3721 caggcatgaa ttggagggcg gtgacaacgc cgagcgcgaa gctgttgtca tgcgtcagga
     3781 tgtggtagac gagccagtcc tgcgtaacgc gtgccatcca ggtgccggta ttcgagacca
     3841 cttgcccgcc cgcgtacagc cggtagttgc gcaccgagag tgcccggaac attcctttca
     3901 agactgcgcc agccgatcga gcagcggcgc tacctggtgc agcagggcgc gctcgtcacc
     3961 ggtcaactgg tcgaagcgag ccgcgagcca ggcatcgcgg cgcttgcggt cttcgcgcaa
     4021 gagggcgagc cctgcatcac tgatcgcgat ccggacctgc cggcggtcga cctcctcggt
     4081 gacccgcgtg agcagtccgc gctcctcgag tattgcggtc gtgcgcgtca ttgacggtgg
     4141 ctggaccttc tcatgcgcgg cgagctcgcc aagcgtcatc gggccgtggc ggtcgaggga
     4201 cccgagaacg gccagttggg ttggtgtgag cccttcgtcg agccgctccg cccgcagtcg
     4261 ccgggccagc cgcatgagtg acacgcgcag ttcgctcgcg agctctgaag ttgcggtctt
     4321 cgaaggcata gttggttagc atagctcatt agctgtgcta aggattggcc gatcccctgg
     4381 acggcggtag cgtgggcaac atggccgccc ctcgccgtcc cgacccgccg cccctacaga
     4441 cgaacgaatt cgcccccgtc atcgtcgcga ccgggctgtg ggttgtcgcg ttcttcatcc
     4501 tgctcagcca gcatcactca ctcgcctcgc acggccacgg ctggtatgtg tgggtcgggc
     4561 tcagcggatt cctgctcggg ttgtggggcc tgtcgctgat gctgctgcgc cgccgcggga
     4621 tacagcgccg ggctcagtcg aggtcggcct cggcgagcag caccgatgac ggcacagcgc
     4681 cacgcagcaa ctcgctcagc acgtaacgcc tcgaccactg gccgtcgcgc caggcacggc
     4741 cacgaccgag cccgaagtcg agcgactgcg catcgacatg caggacaccg tcggcgagcc
     4801 tccacgcgac gcgctgggtc tcaccaccga cagccacgac cagcagtggg tcatgcacga
     4861 cgtgatcgtc gtgcggcacg ccggtgctcg cgacctcgaa ccggccgagt tcgctcgcga
     4921 gcgcgacatc gagtgcgtcg gccacgcggg tcgcgtcgtc aagtgatgcc ggcacgatcg
     4981 cgaattcacc aagcagccca agcaaatccg gcgcgtcgac aacaaccgcg tcgccggccc
     5041 gaaccaccgc gagctcattg cgaagcaccg cccgcaccaa cgccggcgcg tcgcgcggtt
     5101 cgagacgtgc gtacagagcc ctcgtctgat cgcgggttac agtgcgcgcc gggtcgccga
     5161 gtcgcgccgc aatctcgtca tggtcggcat cgtcgagatc gcggagcgcc ccagccgcct
     5221 cgagaaactc gtcgtcaccc tcgatgccta cgacgtcaaa aagcccatgc aataaggggt
     5281 cggacgccgg aagccggccg tcgacaacgc gtcgctcgcg caaccaccac gcggtgtacg
     5341 acggcacccg cgcttgcaag ccaccaccga tgacaactgc gcgatcgacg acggtggctc
     5401 gcaggggcgg cttcgacagc aatgccaacg cggccggcca cgcgtcgtcg cgaacgaatt
     5461 cgagatcgcg gacgccgaca aactcgctca ccacgaccgg ttcgtcaacg gcgagaaccg
     5521 cacgcaccga atcgaggtac tcaccttcga ggtcgaggtc atgatcggct ccgacgacgt
     5581 cggtgtcagt aacgatcgca aagctgtcga gcacgccgac cgcctcgagc acctgcgcgc
     5641 cccaacgctc gaccaatgca ggatcgaccg tgccgaaagg tgcatcgtcg gcgacgacgc
     5701 cagcgagttt cccttgtggc ataaggagtt caccggcgac gaagagctcg ccgtcgtcgg
     5761 ccggcagggc aagttcggcg agccagatct catcaccggc ctggagattc gccgcagcca
     5821 ccaggcgcaa gatcgcatca gcgacctccg ccggctcggc ggcgtcgtag gaattctcca
     5881 ccgccgcccg aacgtggtcg tcggcgagca cggctcgcgg gtcggcctcg accgcaccca
     5941 gccgcaacaa cagcggatgc gcagcgtccg catggattgt tcgcagcccg agcacggtca
     6001 ggtcgccgaa ctgccccggc atcaacaatc ctcgcgctga acgcgcgacg ccgcccgagc
     6061 ccaacggaac aggcaccccc gacagcacgt ctgacgaaat gccggcgccg gccaaggccg
     6121 aatagaggct gtgccaccac gccggctccc gctcgacagt cgcgaggtcg tcgataagag
     6181 tgccgagggg cagccgtcga actcccaggg ctgctagcgc cgtcggattt cgacgcgccc
     6241 acgatgcggg caccagctcg ggcagtaccg gtgccagcac cacgcccagc gagtcgtcgg
     6301 acgccagccc cgcgtcgtcc agcatgacgg cattcgctgg ccgcatccgg atcgaagact
     6361 cagccgtcga cagaaagggg acctcgcgca gcgcgggaag gatgcgcgcc cgcagctcac
     6421 cgtcgatcgc acccgccgcc accgacaccg gcacgaggtc gagcagcgcg gcgctggcgg
     6481 acatgcctcg aacgaggtcg gcgtacgcgg tcgccgcgtg atcgagcaac gcatcgcgca
     6541 acggtccggg cgcgacatgg cggcgcgtcg ggtcgagcgg caacggtgcg acaagcagcg
     6601 ccggcagcgt aagcggctcg tcggtggggg taggcgcgtg gacgacgccg ggtaccgacg
     6661 caggcaacgc gaccggagcg cccgcctcat cgacaggcac cgcccacgtg agcacgaaat
     6721 cgcgccgccc gcgttcttcg accggccgat cacggagcag ttcggcgtcg aaccgtccgt
     6781 tcgtttcgtg cacgatccac cgcgtgccat caacgacaag accgtcggca gtctcgactg
     6841 cgtgaagctc gcgcgccgcg ccgtcaatga cgatctcgac tgtcgcaagc gtcggcaagg
     6901 tgagcagcaa cacggcgtcg acgtcgtcga gcaaggagcg aaccagttca acggccgtcg
     6961 cgtcacgaag gggcagcacg acctcggtgt cgaatccgtc acctggccgc aggccctcgc
     7021 cggcgtaggg aaggcgcagg atcggcagcg ccgtacctcg gcgcgcgacc tcagctgcca
     7081 gggctggtat cgccgccacg tcgtcgcgca ggcggtcgag cgaccacgtg accgagcctt
     7141 cggttgcgac gatcgacggc tcgtcggtca ccgcgagcac ggcggcgaac ccgacgccga
     7201 aacggccaac tgctcccgac gatccctggt ccctcttcga agatgcccgc aacgtcgacg
     7261 ccgattcgac ccctgccgcg tcgatcggcg cccctgtgtt cgaggcgcgc agtacggcgt
     7321 ccgcgctgtc gaggtcaaac cgcaagcggc cccggacgcc ggcccgaccg gccgcatcgg
     7381 ctgcgttttg cgcgagttcg atcagcaccc ggtcgcggta gccgccaagg acgagatcct
     7441 cctcggcgtt cgcgtcctcg cggaagcgtg ccggcgacgc ggcccacgac gccagcacgt
     7501 tggcgcgcag cgccgccgtg ccgaacgggt cgttcagtgc gtgcccgccg cgtcgtcgtc
     7561 cagggcaaac tcagcgactt cgtcgaccgg cacgtcgtcg tcgagtgcga cctcgtcgac
     7621 agtcacgacg tcgacgacct cagccacccc gccgtccccg ccaatctcgt cgatatcgag
     7681 gtcgatggcc tccggcgtgc gcagcggttc ggtctgagca accaacgcct ctgagtgggc
     7741 gccgcagcca tggtcgaacg agaccacgtg gccatcatcg ggcgcgtact cgttggcaca
     7801 cactccgaac acccggctga gcgcgcctct catcagcacc aggaatccgc acgacgcgca
     7861 gtgcagatcg gcggcccgcg cgatcggagc ctcgggcccg cgatcaccct cgtaccagcg
     7921 ctcggcagct tcgagccgac cgaactcaga caggacccgc gggcgaccta gccacaactc
     7981 gttgaccagc gcatcgatcg tgtcgacgtc gtcggtctcg tcatgcgcac cgatcgccgc
     8041 atacccgggc accagccgct cgtcgtcagc cgccgtcgga agcacgtcgc cgacgccgag
     8101 gtcacctgcc agcagccgct cactccacgg cacccaggcc ggcgcgagca acgcatcagg
     8161 cccgggcagc agcaccactt cgtcgaccgt gacggtctta gaacgaggtg cgcggacgac
     8221 ggtcactgcc catcgccagg ccgtgtaggc ccggtcggtg caggcaaaga agtgggtcac
     8281 gatccgctcg gcgtcggctt cgacgcctgc gtggtcgccg acaagctgat ggccagcgac
     8341 cgcctgggct gcccttcgag cgaggtcgac ggctgcggcg cagaccgcat cgggtgccag
     8401 tacaggttcg gctttatcga ccgatggtgc tgtcgtgtcg atcggagtct gcgtcacagt
     8461 ccatgattct cgcatgcatc gcgtgcgcgg cctgtgagta gttgactgct cccgtgtccg
     8521 aaccacgaac gaccgagccc caccagcccg acggctttgg ccgagcggtc gttcacctca
     8581 tcgccggcgc cggcgggggc gcggccaggt ggttcacccg ctccacccgt gccgaagggg
     8641 ccgatgcgag cggcctggcc agcctgatcg atctgcacgc gatccaaagc gcaggcgacg
     8701 cactgctcac ggtcgccttg gcgaacaccc ttttcttctc cgtctccgtc gaccaggcgc
     8761 gcagcaaggt cgctctctac ctgctcatca ccatggcacc cttcgccgtg atcgccccgg
     8821 tcatcggtcc gctcctcgac cggttccgca acggacgccg ttacgcgctg gcggccacct
     8881 tgatcctgcg ggcgttcctg gcgtgggtga tggcgggcgc ggtcggcaag catggcgggt
     8941 tcgccctata tcccgcggct tttggtgcgc tggtgtgctc caaggcctat ggcgtctcgc
     9001 gcagcgcggt cgtgccacgc gtgttgccgc cgaacgtcac cctcgtccgc ggcaactccc
     9061 ggctgacctt gtggggagtg ctcaccgcaa cggtcgcggc accgatcggt cagggactca
     9121 gctgggcgac cgggtcgccg tcgtggacgc ttcggctgtg cgcgctggtc tacctcgccg
     9181 gcagcgtgtt cgccttcaag ctgccggagc gggtcgactc gagcgagggc gaagttcgcc
     9241 tgcgccgccg accacgccga gccagccagc cgcgccgatc cgacgacagc cagcagcgtg
     9301 aggttgcacg ctcgacgtac cggcgcatcc tgcctccgct tcgaggcatc gggccgcgga
     9361 tgaccacgat gctgcgcgcc agcgccggcc tgcgggcatt caccgggttc ctcacccttt
     9421 tccttgcgtt cttgattcgc aagcaccctc tcggcgtctt cgtcccaggg gccgacctcg
     9481 gcatcatcgt cgcctgcgcg gcgatcggca gcgtgctcgg aacgacgctt ggtggctggc
     9541 tgaaacctcg ccagccggag atcttggcga tcgtcagcct gttcgccgca tcgatcatcg
     9601 gcatcgtcac agcgatctgg ttcaacctgg cgacagcggc gctggcgatc ctcatcgcca
     9661 atgtgagcca gtcgctgtcg aagctcggcc tcgactcggt catccagcgc gacgcggtcg
     9721 agcacgtgcg cacctcggcg ttcgcgcgat ccgaaaccgt gcttcagctt tcgtgggttc
     9781 tcggcgggtt cgtggccatc ctgctgccgt cgaacggcag cttgggactc gcgctcgggg
     9841 ccgccgtcct cgtggttgtc cttctttcca cgatgaaagc ggtgcgtacc cgggtgagcc
     9901 ccgaccgtgg catcgagctg ctgcgcgaac ggcggcggcc ggccgtctcg agcgacacgt
     9961 gagagtgctt gtcgtctgcg cggtcgaggc cgagcgcgac gcggtggtgc gcaacttcgg
    10021 tgccgtacgg cccgtcaccg tcgacgggtt cagcggcgtc gctgtcgaca ctgcggccgg
    10081 tgagttgcgt gccttcagcg tcggcgtcgg cccggtggca gctgcgatcg gtacgtcggc
    10141 cctgatagcc ggcatggact atgacctagt ggcgagtgca ggcatcgccg gcggctttcg
    10201 tggccgcgtc gcgatcggcg acgtggtgct ggccaatctc gcgacctatg ccgacctcgg
    10261 cgtccgtgtc gacgatgggt tcctgtcggt gcgcgacctc ggcatcgatc aagactcttc
    10321 gctcgcgttc ggcgaccaac ggctcatcga tcggatgtcg gcgagcggcg tgctcgtcgg
    10381 tgaggtgctt acgctctcag caatgacagg cactgatgcg gacgccgagg agctggcagc
    10441 ccggtatccg agcgcgctcg ccgaggcgat ggagggcttc gccgtggttg ccgcgacccg
    10501 tgaccatggc ggcatcaggt acgtcgccga gatccgcgcg atctcgaatc tgatcggccg
    10561 gcgcgaccgc tcgacctgga acattccgct ggcgttcgac gctcttgcag atgcgatgtc
    10621 gactctgctg aatgaacccc tgccgtgacc gccggacggc tgtcattggc cttctccccg
    10681 tgcccgaacg acacgttcgc gttctacgcc tggacacacg ggctgatccc cgacgcaccg
    10741 ccggtcgaca tcaccttcgc cgatgtcgac gtgacgaacg gcgccgccga gcgtggtgag
    10801 ctcgacgtgg tcaaagtgtc gtacgcggcg ctgccttggt tactcgacca gtacgcgctg
    10861 ttgccgagcg gtggtgcgct cggccgcggg tgcggaccgc ttgtgctcac ggccgggcgc
    10921 cgcgatctgc ggggcgcgtc gatcgtggtg cccggggaac gcaccaccgc ctacctgctg
    10981 tttcggctct gggccgcaaa tcaaggcgtc ggggagatca gcgttttgcc gttcccacag
    11041 atcatgccgg cggttcgcga cggccgcttc gacgccggcc tggtgatcca cgaagcgcgc
    11101 ttcacctaca ccgattacgg tctgacgaac gtgatggatc tcggagcatg gtgggaggca
    11161 gacaccggtc tgccgattcc gcttggcgcg atcatcgcgc gccgatcgct cgacaccgat
    11221 gtgctggcgc gctccgcgcg ggcgtccgtg gaacacgcct tcgcccaccc ctcggcgtcc
    11281 gcctcattcg tcgctgcaca cgcgagcgag atggatcccg atgtacaggc ccgccatatc
    11341 gccttgtatg tcaacgaatt ctcggtcgac ttgggcgacg agggctacgc cgccatcgac
    11401 gcactgttga agcgagccgc cgatgcgggc ctcgtgccac ccgtcgatcc cgcggggctt
    11461 cggtgagcga acagctagcc cttgtacagg cgggcgacca gcccagggcg gttgcgacgc
    11521 tggtgtcggc cttcactgac gatccggtcg aacgctggct ctggccagac gacaatgcgt
    11581 acgcgaccca gttcccacgg ttcgtggctg cattgggcgg cagggccttc gaggtgcaga
    11641 cggcttggcg tctcggtgat ttcgccgcgg tcgcgctgtg gttcgcaccg ggcgtcgagc
    11701 ccgacggcga gctcatcgtc ggcgtactca gcgaaaccgt gccacaggac aagctcgacg
    11761 acacgttcac ggtgctcgaa gcgatggact cagtgcatcc gcagtacccg cattggtacc
    11821 tgccctggtt cggcgtcact gctgccgcgc agggccgagg cctcggcagt cagctcatga
    11881 cccagtgtct ggagatcatc gacgcgagca acctgcctgc gtacctggag accccgaacc
    11941 cgcgcaacat ctcgttctac gagcgccacg ggttcgaggt caccggtgag gtgcaggccg
    12001 gcaattgccc gccggtcacg ttcatgctgc gccccgggca ctagcgggtt tactggtcga
    12061 gg
//
LOCUS       DASWVF010000711         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_224037, whole genome shotgun sequence.
ACCESSION   DASWVF010000711 DASWVF010000000
VERSION     DASWVF010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224037"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..888)
                     /locus_tag="VGD55_12555"
     CDS             complement(133..888)
                     /locus_tag="VGD55_12555"
                     /inference="COORDINATES: protein motif:HMM:NF005095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY0871223.1"
                     /translation="MPSKLDFSKELIGRRALVTGGTRGIGAAIAQRLLDAGAKVVVTA
                     RSRGETLPAGAEFVAGDVASASGVKAIAAGALDALGGLDILVNNAGGGSGFPQGATSI
                     PDEAWQASFALNLFAAIRLTNALLAALRESKAGAIVNVSSGAATMAVGAIAHYAAAKA
                     ALNSYSRALAIELAPSNIRVNVVSPGPVTTPGSREFSDAMGAPPDLFAKEIPLGRYGA
                     PEDLAEVVALLASDRGKWMTGGNINVDGGMSAR"
     gene            complement(1339..2304)
                     /locus_tag="VGD55_12560"
     CDS             complement(1339..2304)
                     /locus_tag="VGD55_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0871224.1"
                     /translation="MRTNVADYETLVGDLHDLRAFALASDLRSLTAVAKLMGESKATT
                     SRRITRLEGALGVALLRRTPRAVETTDDGMLYRLRIAEVLELMGDANAAIRGARVAPA
                     GQLRVTVPPGFGDVLAPVLARFLEEFPKIVLVVDVTARRVDIEAEHYDVGLRATSKLA
                     DSSLVAVRVGKHHERILVAAPSYLTVHAAPRRVQDLASHRIVMIGDVGTTWSIELTRV
                     GGTEQVSVALPTTIAASDVGFARELVLAGAGVAILPRPITDASLDAGTLVHVLPSYAA
                     PSVNLYLLHRGGRFVPPKVRVFVDFVRAALERALDNKVNRPRTRP"
     gene            2394..3050
                     /locus_tag="VGD55_12565"
     CDS             2394..3050
                     /locus_tag="VGD55_12565"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY0871225.1"
                     /translation="MSDIEEARGERVVIEFEGRKCIHSRHCVLSRPDVFVPNVEGAWI
                     HPDRATPEEVAELAHACPSGAISYRRLDGGPEESAPMVNVARIRENGPLAIHGQLQIE
                     GHGESLRVTLCRCGASKNKPFCDSSHATVGFVATGEPAAAESRPLDARAGILKIVPQR
                     DGPLRIEGNLELCAGTGHTINRVIKTALCRCGHSSNKPYCDGTHAKVGFKSDGADRSV
                     "
     gene            complement(3019..3939)
                     /locus_tag="VGD55_12570"
     CDS             complement(3019..3939)
                     /locus_tag="VGD55_12570"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0871226.1"
                     /translation="MRARDFGDLSALAAIVQHGTFARAAAHLRVSPSALSQTIRGLEE
                     RLGVRLLNRTTRSVAPTDAGARLLARVTPAMRELDTAVADVASARGRVAGQLRLNVPR
                     LGGWLIAPMLGRFHDAYPEVVLDVTVEDAVVDIVKGGFEAGVRLGERLQKDMIALKLG
                     GDYEMMAVATPAYFARFGVPTHPRDLHRHRCINWRWPSDGSLYRWEFERGREKLEIAV
                     EGPLVVTDFDLAFQGALAGAGIAYGYDPRIEPAIAEGALQRVLQPWSPSFAGLSLYYA
                     NRRQLTPTLQAFIDFLRGEGLIRSGPRRRF"
BASE COUNT          696 a         1323 c         1282 g          653 t
ORIGIN      
        1 cagtgaagtt tgcgtggaca tgactttttc cgctccctgc accacgaggt gaaacgcccg
       61 tttacaatat ctcaaacgag ccggcagagg tgcgaggagc agtcgcatgt ctcggtgctg
      121 acgacctgac cttcagcgcg cggacattcc accgtcgacg ttgatgttcc cgcccgtcat
      181 ccacttcccg cggtcggacg cgagcaacgc gacgacctcc gccaggtcct ccggggcccc
      241 atagcggcca agaggaattt ccttggcgaa gaggtcgggc ggagcgccca tcgcgtcgct
      301 gaactccctg ctcccgggcg tggtcaccgg ccccggcgac accacattca cgcggatgtt
      361 gctcggcgcg agctcgatcg cgagcgcgcg tgaatagctg ttgagcgcgg ccttggcggc
      421 ggcgtagtgt gcgattgctc cgacggccat cgtcgctgcg cccgacgaga cgttcacgat
      481 ggcgccagcc ttcgattcgc gcagcgccgc caaaagcgcg ttcgtcagcc ggatcgcggc
      541 gaacaggttg agcgcgaagc tggcctgcca cgcctcgtct gggatggacg tggcgccttg
      601 cgggaacccg ctcccgccgc cggcgttgtt cacgaggatg tcgaggccgc caagggcgtc
      661 gagcgctccg gccgctatgg ccttcacccc agaggcggag gcgacgtcgc ccgcgacgaa
      721 ctcggcgccc gccggcaagg tctcgccacg agagcgcgct gtcaccacga ccttcgcgcc
      781 ggcgtcgagc aggcgttgcg cgatcgccgc gccgatgccg cgggtgcctc cggtgacgag
      841 cgcacgacgg ccgatgagtt cctttgaaaa gtcgagcttg ctgggcatga gtttcccttc
      901 gagagaccga tccgtcgaca cggcattcat gagagagggt gctccaagag gttgtgcagg
      961 acgagcggtg cttggaacaa aaggagtcgc cctccctcgt tgatcctccg ttctccgatg
     1021 gtgcgcgtcc caacatcgag cgtctgattg aagctcacgc cgaagtcctt ccggttgatc
     1081 ttcgtggtcg cgacgaagcc agcgcgacgc ttgccccaag gatccttggc tatgacgccc
     1141 gcccctctcg acgtgcagct aaacctcttc gaaccgcctg cgcaccttgc tcacgagcaa
     1201 gtgacgtacc gtgaaatcga tgcccgagtg tgcgtggtcg aagtcccatt tcagtgcgct
     1261 catgccctca aggtgctgcg ttgcgcggat gatggcaatg cgacgatctg caacttcacg
     1321 ttgcagcagc tgcaacgcct aaggacgtgt ccgtgggcgg ttgactttgt tgtcgagtgc
     1381 tctttccagc gcggcgcgaa cgaagtcgac aaagacgcgg accttcggcg ggacgaatcg
     1441 cccgcctcgg tgcagtaggt acagattcac actgggagca gcgtagctcg gcagaacatg
     1501 cacgagcgtg cccgcgtcga gtgaggcatc tgtgatgggg cgcggcaata ttgcgacgcc
     1561 agcgccggcg agcaccagct ccctcgcgaa cccaacgtca gaggccgcta tcgtggtggg
     1621 aagcgcaaca ctgacctgct cggtaccgcc gacgcgcgtg agctcgatcg accacgtcgt
     1681 tccgacgtcg ccgatcatga cgatgcgatg cgatgcaaga tcctgaacgc gacggggcgc
     1741 ggcgtgcacc gtcaggtacg aaggagcggc gacgagaatg cgctcgtggt gcttgcccac
     1801 gcgtactgca accaggctcg aatcagcgag cttggaggtc gctcggagcc cgacgtcata
     1861 gtgttccgcc tcgatgtcca cgcggcgcgc cgtgacgtcg acaacgagga cgatcttggg
     1921 aaactcctct aaaaagcgcg ccagcacggg cgcaaggacg tcaccaaagc ccggcggcac
     1981 cgtcacccga agctgtccgg ccggcgcaac gcgcgcgccg cggatcgcag cgttggcgtc
     2041 gcccatcagc tcgaggacct cggcgatccg caggcggtaa agcatgccgt cgtctgtcgt
     2101 ctcgaccgcc cgcggcgtac gtcggagcaa tgccacacca agcgcgccct cgagcctggt
     2161 gatccgccgg cttgtcgtcg ccttgctctc ccccatcaac ttcgcgacgg cggtcagcga
     2221 cctcagatcg ctcgcgagag cgaacgcgcg gaggtcgtgg agatcgccga ccagtgtttc
     2281 ataatccgca acattcgttc gcatccacac atctttgccg atcctgcgaa acgtcgcatc
     2341 agcacttttg ccaccgcggc ggcggtgcca ccggcctgct agcatccctc gccatgagcg
     2401 acatagaaga ggcgcgcggc gagcgtgtgg tgatcgagtt cgagggccgc aaatgcatcc
     2461 attcgcgtca ctgcgtgctc tcgcgccccg acgtcttcgt cccgaatgtg gagggggcgt
     2521 ggattcatcc ggaccgcgcg actcccgaag aggtcgctga gctcgcacac gcctgtccct
     2581 ccggcgcgat ctcgtaccgg cggctcgacg gcgggcccga agagtcagcc cccatggtga
     2641 atgtcgcacg catccgcgaa aacggccccc tcgccatcca cggccagctt cagatcgaag
     2701 gacacgggga gtccctgcgc gttacgctgt gccgctgcgg agcatcgaag aacaagccgt
     2761 tctgtgacag cagccatgcc accgtcggtt tcgtcgccac gggcgaaccg gccgctgctg
     2821 agtcgcgacc gttggatgcc cgagcaggca tcttgaagat cgtccctcag cgtgacggac
     2881 cgctgcggat cgaaggaaac ctcgagctgt gcgcgggcac cggtcacacg atcaaccgcg
     2941 tcatcaagac ggccctctgc cggtgcgggc attcctcgaa caagccgtac tgcgacggaa
     3001 ctcacgccaa ggtcggattc aaaagcgacg gcgcggaccg gagcgtatga gcccttcgcc
     3061 gcgcaggaag tcgatgaagg cctgaagggt cggcgtgagc tggcgccggt tcgcgtagta
     3121 gaggctgagc ccggcgaacg acggcgacca tggctggagc acgcgctgca gcgccccctc
     3181 ggcgatggcg ggctcgatcc gtggatcgta gccgtaggcg atgcccgccc cggccagcgc
     3241 cccctggaac gcgagatcga agtcggtgac taccagcggc ccctcgacgg cgatctccag
     3301 cttctcgcga ccgcgttcga actcccagcg gtagaggctc ccgtcggaag gccagcgcca
     3361 attgatgcac cgatgccgat gcagatcgcg cgggtgggtc ggcacgccga agcgcgcgaa
     3421 gtaggccggc gtcgcgaccg ccatcatctc gtagtcgccg ccgagcttga gcgcgatcat
     3481 gtccttttgg agacgctcgc cgaggcgcac gccggcttcg aagcccccct tcacgatgtc
     3541 gacgacggcg tcttcgacgg tcacgtcgag cacgacctcc ggatacgcat cgtggaaccg
     3601 gccgagcatc ggggcgatca accatccgcc cagtcgcggg acattcaggc gcagctgtcc
     3661 ggcgacccgc ccgcgcgcgc tcgccacgtc cgcgaccgcg gtgtcgagct ctcgcatcgc
     3721 cggtgtcacg cgcgcgagga ggcgtgcgcc ggcgtcggtt ggagccacgc tgcgtgtcgt
     3781 gcgattgagc aggcggacgc caaggcgttc ttcgagcccg cggatggtct gactgagtgc
     3841 cgacggagac acgcgcaggt gggcggccgc acgtgcgaac gtcccgtgct gcacgatcgc
     3901 cgccagcgcg ctcagatcac cgaaatcacg cgcccgcatt atgtagaaat ttgt
//
LOCUS       DASWVF010000712         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_19953, whole genome shotgun sequence.
ACCESSION   DASWVF010000712 DASWVF010000000
VERSION     DASWVF010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19953"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGD55_12575"
     CDS             <1..289
                     /locus_tag="VGD55_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0871227.1"
                     /translation="YELSPGALVDETLVASGTMTNRVDRLSRRGYVTREPDPADRRGV
                     LVRLTVSGRKKIDTVTAELAKAEAVMWRSVAERKSEQLDGTIRELSATLDR"
     gene            complement(397..2163)
                     /locus_tag="VGD55_12580"
     CDS             complement(397..2163)
                     /locus_tag="VGD55_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0871228.1"
                     /translation="MNLVNLESVSLSYGVRPLLDDVSLGIAEGDRIGIVGRNGAGKTS
                     LIQVLAGASGAHSGRVTHTSGLQIGVLAQGDALDPSASVRDVVLGDRPDHDWAGDRTL
                     RSVVSTLLREDMLDRLVGTLSGGERRRVALAALLVGNIDLLMLDEPTNHLDIETIDWL
                     ARHLSTRRGALVVVTHDRWFLDAVCTLTWEVADGRVGQYEGGYAAYVLARAERERVSA
                     TTEDRRQNLLRKELAWLRRGPPARTSKPRFRIDAANALIADEPPARDRTVLAEFSSVR
                     LGKSVFDVEDASLELGDAELLDDVTWRVGPGDRIGLVGVNGSGKTTLLRVLAGLRDVD
                     TGVVKTGTTVRIGYLSQDVAELDPTMRVLEAVESVRSRTVLGDGRELTASALLERFGF
                     TGERQWTPVGELSGGEKRRLQLLRLLMDEPNVLLLDEPTNDLDVDTLTALEDLLDNWA
                     GTLIAASHDRYFIERVCDSVTALIGGGKLASLPGGVDEYLARRSASVSVPLTPAAAAV
                     AAGAGTSGSGDVRAARKDLTRLERQLTKLTEREALLHSELAAAATDYVKAAELDAQLR
                     ALHESRAQLEDEWLDAATRAGR"
     gene            complement(2160..3056)
                     /locus_tag="VGD55_12585"
     CDS             complement(2160..3056)
                     /locus_tag="VGD55_12585"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0871229.1"
                     /translation="MSVSVRAPAKVNLHLGVGPLRDDGFHALSTVFQAVSLHDEITVD
                     DADDLGVAVVGEGVDEVPTDMSNLAVRAAVALAHHVGRAPAVKITIRKGIPVAGGMAG
                     GSADAAATLVACDALWETGLEPEVLHDIAMRLGSDVPFCLIGGTALGAGRGEQLTRIL
                     VRGTYHWVFAIADTGLSTPAVYGELDRMRAEGPWPETSTPRELIAALGSGDARRLGPL
                     LHNDLQRSALRLKPALRRTLEAGRDAHALGAIVCGSGPTCAFLTYGEPEAVALVAALA
                     GSGTCRTVRRAYGPVPGARVVV"
     gene            complement(3070..>3311)
                     /locus_tag="VGD55_12590"
     CDS             complement(3070..>3311)
                     /locus_tag="VGD55_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871230.1"
                     /translation="DVFAVIDAAFGQRRKTLRAALAGWAGSPAAAEIALRAAGIDPGA
                     RGETLDVTSFARIAEQRPEQPPAPSSGPPVPPARS"
BASE COUNT          581 a         1157 c         1072 g          501 t
ORIGIN      
        1 atacgagctg tcacccggtg cgctggtcga cgagacgctc gtggcgtcgg gaacgatgac
       61 caaccgtgtc gaccgcctca gccggcgcgg ctacgtgacg cgtgaacccg acccggccga
      121 ccgtcggggt gtgctggtgc ggctcaccgt cagcggtcgg aaaaagatcg acaccgtcac
      181 tgccgaactt gccaaagccg aagccgtcat gtggcgcagc gtcgccgagc gcaagagcga
      241 gcagctcgac ggcaccatcc gcgaactatc ggcgaccctc gaccgctaga ccgttcgtga
      301 tcatgcacat agttggcacg accgaacact ccggtaccac atttgcgcat gatcatcatc
      361 gcgaacggtg gctcggaagg gttggggtgc cggtgactag cggccggcgc gggtggcggc
      421 gtcgagccat tcgtcctcga gttgcgcacg tgactcgtgc agcgcgcgca gctgtgcgtc
      481 gagttcagcg gccttgacgt agtcagtcgc ggccgcggcg agctcggaat gcagcagagc
      541 ctcgcgctcg gtcagcttcg tcagctgccg ttcgaggcgc gtcaaatcct tgcgcgctgc
      601 acgcacgtcg cccgacccgc tagtacccgc acccgcagct acggcagcgg cagccggtgt
      661 caacggcaca gacaccgagg ccgagcggcg ggcgaggtac tcgtcgactc ccccgggcaa
      721 gctcgccagc tttccgccgc cgatcaacgc ggtcaccgag tcgcacaccc gctcgatgaa
      781 gtaccggtcg tgcgaggccg cgatcagcgt gcccgcccag ttgtcgagca agtcctcgag
      841 cgcggtcaac gtgtcgacgt cgaggtcgtt ggtcggctcg tcgaggagca acacgttcgg
      901 ctcgtccatc agcagccgca gcagctgaag tcgccgcttc tccccacccg acagctcacc
      961 taccggggtc cactggcgtt cgcccgtaaa gccgaaacgc tccaacagcg cggacgccgt
     1021 cagctcgcga ccatcgccaa ggaccgtcct gctacgtacc gattcgaccg cctccagtac
     1081 ccgcatcgtc ggatcgagtt cggcgacgtc ttgtgagaga tagccgatac gcaccgtcgt
     1141 cccggtcttc accacgcccg tgtcgacgtc tcggaggccg gcgagcacgc gcaacagcgt
     1201 ggtcttgcca ctgccgttta cgccgaccag gccgatccga tcccccgggc caacccgcca
     1261 ggtgacgtcg tcgagcagct cggcatcgcc tagctccaac gaggcgtcct cgacgtcgaa
     1321 gaccgacttc ccgagccgca cagaggagaa ctccgcgagc acagtgcggt cacgcgccgg
     1381 cggttcgtcg gcgatcagcg cattggcggc gtcaatgcgg aagcgcggct tcgaggtgcg
     1441 cgcgggcggt ccgcgccgca accacgcgag ctctttgcgc agcaggttct gccggcggtc
     1501 ttcggtcgta gcagaaacgc gttcacgttc tgcacgagcg agaacgtagg ccgcgtagcc
     1561 gccctcgtac tgaccgacgc gaccgtcggc gacctcccat gtcaatgtgc acaccgcgtc
     1621 gagaaaccac cggtcgtggg tgaccacgac cagtgcaccc ctgcgggtcg agaggtgtcg
     1681 cgccaaccaa tcgatcgtct cgatatcgag atggttcgtc ggctcgtcga gcatcagcag
     1741 atcgatgttg ccgacaagca acgcggcgag cgcgacccgt cgtcgctcac cgccggacaa
     1801 ggtgcccacc agccggtcga gcatgtcctc gcgcagcagt gtgctgacca ccgaacgcag
     1861 ggtgcgatca ccggcccagt cgtggtcggg ccgatcgccg agcacgacgt cgcgcaccga
     1921 tgctgagggg tcgagcgcat cgccttgcgc caagacaccg atctgcaacc ctgacgtgtg
     1981 cgtgacgcgc cctgagtgcg cgcccgacgc gcccgcgagc acctgaatca gcgatgtctt
     2041 gcccgctccg ttgcgaccga cgatgccgat gcggtcacct tcggcgatgc cgagcgacac
     2101 gtcgtcgagc aacggccgga caccgtagga cagcgatacc gattcgagat tgacgaggtt
     2161 catacgacga cgcgagcccc tggtaccggt ccgtaggcgc gccgcactgt gcggcaagtg
     2221 cccgaccctg caagtgccgc taccagcgcg acggcctccg gttcgccata ggtcagaaac
     2281 gcgcaggtgg gtcccgagcc gcacacgatc gcaccgagtg catgggcgtc gcgacctgct
     2341 tcgagcgtgc gacgaagcgc cggcttcaac cgcagcgccg acctttgcag gtcgttgtgc
     2401 agcagtggtc cgagccggcg agcatcgccg gatcccaacg cggcaatcag ctcgcgcggc
     2461 gttgacgtct ctggccacgg gccttctgca cgcatccggt cgagttcgcc gtagacggcc
     2521 ggggtcgaca gcccggtgtc ggcgatggcg aaaacccagt ggtaggtacc cctgaccaga
     2581 atgcgcgtca gctgctcgcc gcgacctgca ccaagcgccg tcccgcctat gagacagaaa
     2641 gggacgtcgc taccgagccg catcgcgatg tcgtggagca cttctggttc gaggccggtc
     2701 tcccacagcg catcgcaggc gaccagcgtc gccgccgcat cggccgagcc accggccatg
     2761 cctccggcca ccgggatgcc cttgcgaatc gtgattttca ctgccggcgc gcgaccgacg
     2821 tgatgggcca atgcgacggc agcgcggacg gcgagattcg acatgtccgt ggggacctcg
     2881 tcgacaccct cgccaacgac agcgacgccg agatcgtcgg cgtcgtcgac agtgatctcg
     2941 tcgtgcaacg agaccgcctg gaacaccgtc gacagcgcat ggaatccgtc atcgcgaagc
     3001 gggccgaccc cgaggtggag gttgaccttc gcgggtgcgc gcacgctgac agacacaacg
     3061 cacccgatgc taactgcgtg cgggtggcac gggagggcca gaggacggcg ccggtggctg
     3121 ctccggccgc tgctccgcga tccgggcgaa ggacgtcacg tcgagagtct ccccgcgtgc
     3181 ccccggatcg atgcccgcag ctcgcagggc gatctcagcc gctgccggcg atccagccca
     3241 gcccgccaac gccgcgcgca acgtcttgcg gcgctgtccg aatgcggcgt cgataacggc
     3301 gaaaacatct t
//
LOCUS       DASWVF010000713         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_96296, whole genome shotgun sequence.
ACCESSION   DASWVF010000713 DASWVF010000000
VERSION     DASWVF010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96296"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGD55_12595"
     CDS             <1..234
                     /locus_tag="VGD55_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0871231.1"
                     /translation="GLGHRRLLCEGGPTLLAAVAADGVLDELCLTIAPMLVGGPSPRV
                     LDGPVLTPPRRLRLTQLLQDDDDLLFARYEVLR"
     gene            231..1307
                     /locus_tag="VGD55_12600"
     CDS             231..1307
                     /locus_tag="VGD55_12600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0871232.1"
                     /translation="MKLPVMPPVKPMLAKSQPSIPPGMVYEPKWDGFRCIVFRDGEEI
                     ELGSRNEKPLTRYFPEVVEAVRASLPMRCVLDGEIIIMSGNSLDFEALLQRIHPAASR
                     VKKLSIETPATFVAFDLLALGDDSLVDEPFSKRRELLEGVIGSASAVLLTPATDSLEL
                     ATRWFEVFEGAGLDGVVAKSRTQPYQQDQRTMVKVKHERTADCVVAGFRWHRASPEGT
                     AVGSLMLGLYGDNGVLQHVGVCGAFSAVRRRELIAELEPYRTDDVAHPWNYSEVTEGA
                     EPGEPERRPGGVTRWNAGKDLSWVPLRPELVCEVAYDHMERGRFRHTAQFRRWRPDRE
                     PESCTYEQLDRPVNFDLARVLSGT"
     gene            complement(1556..2584)
                     /gene="ligD"
                     /locus_tag="VGD55_12605"
     CDS             complement(1556..2584)
                     /gene="ligD"
                     /locus_tag="VGD55_12605"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0871233.1"
                     /translation="MAPSPAIELEIEGRAVRVSNPDKVYFSGKGQTKLDLVNYYLSVA
                     PGIVRALFERPCTLQRFPDGVEGEAIYQKRVPENHPEWLETARVTFPSGRHADELCVT
                     EVASVAWAANLGTVVFHPWPSRRADTEHPDELRIDLDPQPGTDFDDARRVAVAVHELL
                     DEVGYIGFPKTSGGRGIHIYVRLQPRWTFTEIRRAVLAFSREIERRIPDDVTTAWWKE
                     ERGAKVFLDYNQNARDRTIAAAYSVRANVRATVSTPITWQELADVEPNDFTMDTVPQR
                     FAKIGDLHAGINDVAHDLTPLTEMADRDERDRGLGDAPYPPNFPKMEGEPSRVQPSRA
                     RRPKPEAS"
BASE COUNT          475 a          896 c          905 g          506 t
ORIGIN      
        1 gggctcggcc accggcggct cttgtgtgag ggcgggccga cactcctcgc agcagtcgca
       61 gctgacggcg tcctcgatga attgtgtttg acgattgcac cgatgctggt gggcggaccg
      121 tcaccacgcg tgctcgacgg cccagtcctt accccgccgc ggcgcctgcg tttgacccaa
      181 ctgttgcaag acgacgatga cctgcttttc gcgcgttatg aggtgctccg atgaaactcc
      241 ccgtcatgcc gcccgtgaag cccatgctgg cgaagtcgca gccgtcgatt ccgccgggaa
      301 tggtctacga gccgaagtgg gacgggtttc gttgcatcgt gttccgcgac ggcgaggaga
      361 tcgaactcgg cagtcgcaat gaaaagccgc tcactcgcta tttccccgaa gttgtcgagg
      421 cggtgcgagc aagtctgccg atgcgctgcg tgctcgacgg cgagatcatc atcatgtcgg
      481 gcaactctct cgatttcgaa gcactgctcc agcgaatcca ccctgccgcg tcgcgggtga
      541 agaagctgtc gatcgagacg ccggcaacat tcgtggcatt cgacttgcta gccctcggtg
      601 acgactccct cgtcgacgag cccttctcga aacgccgcga gctgctcgaa ggtgtgatcg
      661 ggtcggcatc ggccgttttg ctgacgccgg cgaccgactc cctcgaactg gccactcggt
      721 ggttcgaggt cttcgagggt gcgggcctcg acggtgtcgt cgccaagtcg cgcacccagc
      781 cgtatcagca agaccagcgc acgatggtga aggtgaagca cgagcgcact gccgactgcg
      841 tggtggcggg ctttcgctgg caccgtgcgt caccagaagg caccgcggtt ggttcgctga
      901 tgctcggcct atacggcgac aacggggtgc tccagcacgt tggcgtctgc ggtgcgttct
      961 ccgccgtccg acggcgcgag ctgatcgccg agctcgagcc ctatcgcacc gatgacgtcg
     1021 cccacccgtg gaactactca gaggtgaccg aaggggccga gcccggcgag ccggagcggc
     1081 gtccgggcgg tgtcacccga tggaacgccg gcaaggatct gagctgggta ccattgcgac
     1141 ccgagcttgt gtgtgaggtc gcgtacgacc acatggaacg cggccggttc cggcacaccg
     1201 cccagttccg tcggtggcgg cccgaccgcg aacccgagtc gtgtacctat gagcaactcg
     1261 accgcccggt caacttcgat ctcgcgcggg tgctcagcgg cacctaggca cgtctcggcg
     1321 agaagtcaga catgcgcgct atcgccgcgc ctgctgtgcg tcgggtggcc gctgacgtcg
     1381 cgacgtcacc gtcccgcaag ccgcagaccg gctcaccaca atgatcgatc ccgagttcta
     1441 ccgactcacg gtcggcgaac ggcactggac gacgaagcag tacgaaagct ggctcacgga
     1501 gttgctcatc gcctcgctcc tgccgactgc actgtcctag tcgacgcgta gaacgtcagc
     1561 tagcttcggg tttcggccga cgggcgcgac tcggttgaac cctcgacggc tcgccctcca
     1621 tcttcgggaa gttcggcggg tacggcgcat cgccgagtcc gcggtcgcgc tcgtcgcggt
     1681 cggccatctc ggtcagtggc gtgaggtcgt gcgcgacatc gttgatgccg gcgtgcagat
     1741 caccgatttt cgcgaaccgc tgcggcacgg tatccatcgt gaagtcgttc ggttcgacgt
     1801 ccgccaattc ttgccaggtg atcggagtcg acaccgttgc gcggacattc gcccgcacgc
     1861 tgtacgcagc ggcaatcgtg cggtcgcgcg cattttggtt gtagtcaagg aaaaccttcg
     1921 ctccgcgttc ctccttccac cacgcggtgg tcacgtcgtc ggggatccgg cgttcgatct
     1981 cgcgcgaaaa cgcgagcact gcacggcgaa tctcggtgaa cgtccagcgc ggttgcagcc
     2041 ggacgtagat atggatgccg cgaccgcccg aggttttcgg aaatccgatg tagccgactt
     2101 catcaagcag ttcatggacg gcgacggcaa cccttctggc atcgtcaaag tcggtaccgg
     2161 gctgaggatc cagatcgatg cgtagctcgt cgggatgctc ggtgtcggcc cgccgcgacg
     2221 gccacgggtg gaagacgacg gtgccaagat tcgcggccca ggcgaccgac gcgacctcgg
     2281 tgacgcagag ctcgtcggcg tggcggcccg acggaaaggt gaccctcgcc gtttcgagcc
     2341 actccgggtg gttctcgggc acccgcttct ggtagatcgc ctcgccctcg accccgtcgg
     2401 ggaagcgctg cagcgtgcat ggtcgttcga acagcgcgcg cacgatgccc ggcgctacgg
     2461 agaggtagta gttgaccagg tcgagcttgg tctgcccctt ccccgagaag tagaccttgt
     2521 cggggttgga gacacgcacc gcgcgtccct cgatctcgag ctcgattgcc ggggaaggag
     2581 ccacgagaag acgctaccgc ccgtccgcgg gttgtaatct tcaccgaccc accgggagtt
     2641 gcggactcac gtaaactcaa gggcatgtac aaggttgaga agacagacga ggaatggcgc
     2701 gctcagctca cgccgtccga atatgccgtg ttgcgcaagg ccgccacgga gcccgccttc
     2761 gtcggcgagt acaccgacac ga
//
LOCUS       DASWVF010000714         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_198353, whole genome shotgun sequence.
ACCESSION   DASWVF010000714 DASWVF010000000
VERSION     DASWVF010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198353"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGD55_12610"
     CDS             <1..438
                     /locus_tag="VGD55_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0871234.1"
                     /translation="DGPGGGSVAADAGSFAGERLGLPQDGPRSVAGMGRRLGALFIDW
                     VIALLIVSPFTGHRPFAPGSNSPWVLAVFAAEYIILVSLIGRTIGMRLCGIAVMRLDG
                     GRVALPAVVVRTFLLLLVVPAVIYDRDRRGLHDKAAGCVVVRL"
     gene            complement(454..1206)
                     /locus_tag="VGD55_12615"
     CDS             complement(454..1206)
                     /locus_tag="VGD55_12615"
                     /inference="COORDINATES: protein motif:HMM:NF025199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HEY0871235.1"
                     /translation="MAKPEKNAPPLKGRAGRKARRAAGKAARAGQPKRSAQIRDAFVM
                     TRSRDKRLLPYLALTFVVSLGFFELIGLLLSDTIIFTVFGVISAALATFIVFGQRAQR
                     AALSDIEGQPGAAAAIITNMRGDWRLTPNVAANRTMDVVHRVVGKPGVILIGEGSTDR
                     LQPMIADQRKRVQRVAAGTPIEVIIVGRGAGQVPLQKLQRKMTRLPRTFKGPTVDEIE
                     GRMRALGAFAIPIPKGPMPKGARLPKGVKPPR"
     gene            complement(1215..2150)
                     /gene="lipA"
                     /locus_tag="VGD55_12620"
     CDS             complement(1215..2150)
                     /gene="lipA"
                     /locus_tag="VGD55_12620"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0871236.1"
                     /translation="MSTIAPDGRKLLRVEARNAAVPIEKKPSWIKTRARMGPEYTALK
                     DLVRSEGLHTVCEEAGCPNIFECWEDREATFLIGGATCSRNCSFCQIDSGRPEPLDRD
                     EPRRVAESVKTMGLRDATVTGVARDDLDDEGSWLYAQTVREIHAAVPGCGVELLTPDF
                     HARPELLAQVFDASPEVFAHNLETVPRIFKSIRTGFRYDRSLAVIELARDRGLVTKSN
                     LILGLGERPDEITSTLVDLRDAGCELVTITQYLRPSLRHHPVERWVEPSEFADLEVQA
                     RELGFVGVMSGPLVRSSYRAGRLYRDALARRASLS"
     gene            complement(2147..2788)
                     /gene="lipB"
                     /locus_tag="VGD55_12625"
     CDS             complement(2147..2788)
                     /gene="lipB"
                     /locus_tag="VGD55_12625"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0871237.1"
                     /translation="MIAFRQLGVVAYDDAWQLQRDVHAQRVADEIPDTCLLLQHPHVF
                     TAGRRTQDFERPVDGTPVIDVDRGGRITWHGPGQLVGYPIVRLPMPVDAVGHVRRLEE
                     ALIAVCAEFGVEAIQIAGHSGVWVQGRNGAAARKIAAIGVRISRCTTMHGFGLNCDPD
                     LSWFGRIVPCGIDDADVTSLSRELGRDVTVDEVTPWVEQHLGEALNVNRRLSA"
     gene            2885..>4062
                     /locus_tag="VGD55_12630"
     CDS             2885..>4062
                     /locus_tag="VGD55_12630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0871238.1"
                     /translation="MDDFLLPGYDVEELVGFGGSGEVWRAREVASGDVVALKRLRAGS
                     SADAASQQASEHRLSREAALLATISHEHIVALRSVVPTDDGLVLVLEYAEGGSLAALI
                     AARGRLSAGEVITIGAPLAQALGDVHARGIVHGDVTPANVLFDGSGKPLLADLGVASL
                     SGDRAGPPGGTPGYADPALDTSVSARPASDVHGLAAVCFSALAGVPPYLEGSAVAQPL
                     RPLAPGTPAALVAAIEAALAPDPAFRPDANAFARELFAACAPVAVRLVHMSAPVVNPL
                     TRDLGGHPRPAPPEPAASDSAPGRHRWSAPRAVVVRRVITAVCALTLLGAAVAAGVGW
                     AWAGDGHRASASPDAADVTPAPSASPAAVTTPATVVTPAAVLPTDWTAVLSALDSARD
                     A"
BASE COUNT          562 a         1359 c         1439 g          702 t
ORIGIN      
        1 gatggccctg gcggtggctc ggtcgcggct gatgccgggt cgttcgccgg cgagcggctc
       61 gggttgccgc aggacggtcc ccgatcggtc gctggcatgg gtcgccgcct cggtgcgctc
      121 ttcatcgact gggtcatcgc attgctcatc gtcagcccgt tcaccggaca ccgtccgttt
      181 gccccgggca gcaattcgcc gtgggtcctg gccgtgttcg cggcggagta catcatcttg
      241 gtgagcctga tcggccgcac gatcggcatg cgcctgtgcg gcatcgcagt catgcggctg
      301 gacggcggga gggttgcgct ccctgcagtc gtcgtccgta cgttcttgct gctgctcgtc
      361 gtgccggccg tgatctatga ccgtgaccgc cgcggcttgc acgacaaagc ggccggctgc
      421 gtcgtcgtcc gcctatagag ctctggtcgt gttctagcga ggcggcttga cgcccttggg
      481 taaccgcgcg cccttgggca tcgggccttt ggggatcggg atcgcgaagg ctccgagcgc
      541 ccgcatgcga ccctcaatct cgtcgaccgt cgggcctttg aatgtgcggg ggagcctggt
      601 catcttgcgc tgcaacttct gcaacggaac ctggccggcc ccacgtccga cgatgattac
      661 ctcgatcggc gttcctgcgg cgacgcgctg cacccgcttg cgctggtcgg cgatcatcgg
      721 ctggagccgg tcggtggaac cttcgccgat gagtatgacg ccgggctttc cgacgacgcg
      781 gtggacgacg tccatcgtgc ggtttgcggc gacattcggg gtgaggcgcc agtcgccgcg
      841 catgttggtg atgatcgcgg cggctgcacc gggctggcct tcgatgtcac tcagcgccgc
      901 acgctgtgcg cgctggccga agacgatgaa cgtcgccagc gccgcgctga tgacaccgaa
      961 aacggtgaag atgatcgtgt cgctgagcag caggccgatc aactcgaaga agccgaggga
     1021 aacgacgaac gtgagcgcga ggtagggcag cagccgcttg tcgcgcgagc gggtcatgac
     1081 gaaggcgtcg cggatctgcg ccgaccgttt tggctgaccg gctcgtgcgg ccttcccggc
     1141 ggctcggcgt gccttgcgtc cggcgcggcc cttcaggggc ggggcgttct tttcgggttt
     1201 tgccatggtg ggcctcagga tagcgaggcg cgccgtgcga gtgcgtcgcg gtagagccgg
     1261 ccggcccggt aggaggaccg gaccagcggg ccggacatga ccccgacgaa accgagctcg
     1321 cgggcctgca cctcgaggtc ggcgaactca gacggctcga cccagcgctc gaccgggtgg
     1381 tgtcgcaagg acggccgcag gtactgggtg atcgtcacaa gctcgcagcc ggcgtcgcgc
     1441 aggtcgacca gcgtcgaggt gatctcgtca ggccgttcac cgagaccgag gatcaggttc
     1501 gacttggtga ccaggccgcg gtcacgcgca agctcgatga ccgcgagcga gcggtcgtag
     1561 cgaaacccgg tgcggatcga cttgaagatc cgcggcacgg tctcgaggtt gtgcgcgaac
     1621 acctcagggc tcgcgtcgaa gacctgagca agcagctcgg gccgcgcgtg gaagtcgggt
     1681 gtgagcagct cgacgccgca gccaggcacc gcggcgtgaa tctcgcgcac ggtctgcgcg
     1741 tagagccacg aaccttcgtc gtcgagatcg tcgcgcgcga caccggtcac ggtcgcgtcg
     1801 cgaaggccca tggtcttgac cgactcggcg acccggcgag gttcgtcgcg atcgagcggc
     1861 tcgggtcggc cgctgtcgat ctggcagaac gagcagttgc gcgagcaggt ggcgcccccg
     1921 atcaggaacg tcgcctcgcg gtcttcccag cactcgaaga tgttcgggca gccggcctct
     1981 tcgcacaccg tgtgcagccc ctcgctgcgg acgagatcct tcaacgcggt gtactcggga
     2041 cccatgcggg cgcgggtctt gatccacgac ggcttcttct cgatcgggac cgccgcgttg
     2101 cgagcctcga cccgcagcag cttgcgcccg tcaggcgcga tggtgctcac gccgacaacc
     2161 tccggtttac gttcagcgct tcgccgagat gttgctcgac ccacggcgtc acctcgtcga
     2221 ccgtgacgtc gcggccgagt tcgcgcgaca gtgacgtgac gtcggcgtcg tcgatgccgc
     2281 acggcacaat ccgaccgaac cagctgaggt cgggatcgca gttcagcccg aacccgtgca
     2341 tggtcgtgca acgcgatatc cggacgccga tggccgcgat cttgcgagcc gctgcaccgt
     2401 tgcgaccctg cacccagacg cccgagtggc cggcgatctg gatcgcctcg acgccgaact
     2461 cggcgcacac ggcgatcagt gcctcctcga gtcgccgcac gtgaccgacc gcgtcgaccg
     2521 gcatgggcag gcgcacgatc gggtagccga cgagctggcc gggaccgtgc caggtaatgc
     2581 ggccgccccg atcgacatcg atgaccggcg tgccgtcgac cggccgctcg aagtcttggg
     2641 tgcgacggcc cgcggtgaag acatgcgggt gctgaagcag gaggcaggtg tcgggaatct
     2701 cgtcggccac ccgctgagcg tgaacgtcgc gttgcagctg ccacgcgtcg tcgtacgcca
     2761 cgacgccgag ctgcctgaac gcgatcacgg gtccacgata cgtggtcgga cggcgggtgg
     2821 tgatcgtccg cgcgcctgtg gacgacggcc ggcggctggc cgcattctgc ggtggaatgc
     2881 cggagtggac gatttcttgc tgccgggtta cgacgtcgaa gagctggtgg gtttcggcgg
     2941 atcgggcgaa gtgtggcggg cacgcgaggt cgccagtggt gacgtggtcg ctctgaagcg
     3001 gctgcgcgct ggttcttcag ccgacgcggc gtcgcagcag gcatctgagc atcggctctc
     3061 gcgcgaggcg gcgctcctgg caacgatcag ccacgaacac atcgtcgcgc tgcggtctgt
     3121 cgtcccgacc gacgacggtc tcgtcctcgt tctcgagtac gccgagggcg gcagtctggc
     3181 cgctttgatt gccgcccgtg gtcgactttc cgctggcgag gtcatcacga tcggagcgcc
     3241 gctggcccaa gccctgggcg acgtccatgc tcgcggcatc gtccacggcg atgtcacacc
     3301 agccaacgtg ctgttcgacg gttcggggaa gccgctgctc gccgatctcg gggtcgcgag
     3361 cctctcgggt gaccgagccg gcccgcctgg tggcacgcct gggtacgccg acccggcact
     3421 cgacacgtcc gtttccgcca ggcccgcgag tgacgttcac gggttggccg cggtctgctt
     3481 cagcgccctg gccggcgtcc cgccgtatct ggaaggcagc gcggtcgctc agccgctgcg
     3541 gccgcttgcg cccggaacgc ccgccgcgtt ggtggcggca atcgaagccg cgcttgcgcc
     3601 tgatcctgcc tttcgacccg acgcgaacgc gttcgcgcgg gagttgttcg cggcgtgcgc
     3661 tccggtcgcg gtgcggctcg tccacatgag cgcgcccgtt gtgaatccgc tgacgcgcga
     3721 cctcggtgga caccctcgtc ctgcgccgcc agaacctgcc gcgagtgact ctgctcctgg
     3781 tcgccacagg tggtcggcgc cccgtgctgt tgttgttcgg cgagtgatca cggcggtgtg
     3841 cgccctgacc ctgctcggtg ccgccgtcgc ggctggggtc gggtgggcgt gggcgggcga
     3901 cggccatcgc gcgtcggcaa gccccgacgc ggccgacgtg actcccgcgc cttcggcaag
     3961 tcccgcagct gtcacgactc ccgcgaccgt cgtaactccc gcggccgttt tgccgacgga
     4021 ttggaccgcc gtcctctcgg cgctcgactc ggcgcgcgac gc
//
LOCUS       DASWVF010000715         5741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_299985, whole genome shotgun sequence.
ACCESSION   DASWVF010000715 DASWVF010000000
VERSION     DASWVF010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299985"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGD55_12635"
     CDS             <1..739
                     /locus_tag="VGD55_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0871239.1"
                     /translation="PMASALPAGMDEDRVAAMSAALLSLAERAAQGLGRGDLSQVYIE
                     GENGTVFLVSAQDEAVLIAVTAADTKVGMMLYEVKLAANKVGEVLRGPAPLPAPLPSH
                     LTRSYLDEAAAETDSAAYEPAEPASDELPYGEADHSQPSYEQPAYVDPAYEAAVEAAE
                     ASEPTHDEPAYSEPVYTSTSNESADVPAESDEDEYAEVAYTSASALTEPYFAEAPATP
                     APPADPPGWSSYAPSGSVLPGGQGSWS"
     gene            816..2207
                     /locus_tag="VGD55_12640"
     CDS             816..2207
                     /locus_tag="VGD55_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0871240.1"
                     /translation="MKLEGSLDAFSLPDIFQLLSFTKKSGGLHLRRGNVHGTVYFRDG
                     SVTGASSDGGRQALARRLVGSGAVGDDELSAAVERAANDGVGVGRALLDAGAVDADLL
                     RNLVAEQAVDAVFDLLRWSEGDFSFTVDEPGPDDVGFSLGVEPIVNDARSRLETWEHA
                     CRVIPSPETVVALPVAVREDPQISRGEWALLALVDGRRSVAQLVALAGRGDYSVVSQL
                     AALIERGLLAARTPDDAEGVLALARRQAILGRLEGDGSAAAVVVDLPDTADVPSTADA
                     VPATVFAPLPATFGAEAAEFVALGEAVDSADTVDANGNLAGAGLAEVSAASTVASVLA
                     AANGESSPFARTQTPSSLRSADDVRAPNAERVPAAERPAARVEERIAAHLVADTRTPV
                     TPSRPEPFMPKRRPEFPDDSSHSAAARMNGGGLATATAAVADPFGSTSQIERDPSVNK
                     SLLLRLIAGVRGL"
     gene            2209..2787
                     /locus_tag="VGD55_12645"
     CDS             2209..2787
                     /locus_tag="VGD55_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEY0871241.1"
                     /translation="MAIKRGRRGGNAQAVKIVVTGPFSAGKTTLIRTISEITVLSTEK
                     DITDETRSTKAETTVAMDFGRITIDHDLVLYLFGTPGQDRFDFMWEILGEGMLGYVLL
                     VDAQRPESLSEAVAILAAFKKMARVPFVVGLNRAQGADPEVESNVRAVLGLDDSVPVV
                     PCDATDRQSVKDVLLALLYAVVDSLDVAAAEA"
     gene            2827..4293
                     /locus_tag="VGD55_12650"
     CDS             2827..4293
                     /locus_tag="VGD55_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0871242.1"
                     /translation="MTALAAPPVSSRSPVTAPVQKAIIPGILVGLAVAYVTMPFAHAT
                     GGREPWALTKAAIVVGVALIAVKPWRELSARVLGLAVAVSLAALAVCLVTPPGWFDGT
                     RAASYALAAATFVTVAAYARSVRRSYPIAAIVATAGAVQFCWSFVAWWGGRDQSIPMV
                     GTFYWHNQFAAFLLAPALIALALLVGRRAPWRLVGWVVAPLAVAGVVHSSSRGGMGVL
                     LAGWLVIGVLAVRVRPNRRAFVKWLAASVLAAGVTVAVSSPPFFSSTASPLSATQARA
                     STGETADANGAYRVQMWRESITVFEHHPVTGVGYGGLLSAASKLTPATWARSPLAHDD
                     YLQALAEGGLLLGLPFLGACGVIAIGLLRVGSSRVHSRVIDVRTGVVVAAIALMAHAA
                     IDFDWTYPALFCLAAVVTALAVGPVLRRPPRSVGATSSDAVGLPARPVVPWARVALVG
                     LLVGATVAGGIAEHRGGLQIAYNVTNANPASASGTGHG"
     gene            4290..>5741
                     /locus_tag="VGD55_12655"
     CDS             4290..>5741
                     /locus_tag="VGD55_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0871243.1"
                     /translation="MKQLGDILLENGLVSGTQLATAYEEHQRSGRSLGRVLVEQGVLS
                     EGQLVAALATQIGLRFVDLTEFSVDGSAVSRVPEAVCRRYTALPVGYDDGKLLVAMAD
                     PANVFAIDDIRSITGLDVKPAVATRADVLSAINRYHRADGELDDLTLSLSAGDDDLDE
                     LSNVKDLVEDAPIVKFVNLLISQAVQDRASDIHIEPTERDLRVRYRIDGVLHEVMKSP
                     KSIQAGVTSRLKIMAEMNIAERRIPQDGRLSVNVTGKKIDLRVASLPTVWGEKIVMRI
                     LDNSTALLKLTDLGFSDGNYERWSQSFTKPYGMMLVTGPTGSGKSTTLYATLNIVSTP
                     DKNVITVEDPVEYRIPGINQVQTNAKAGLTFASALRSILRSDPDVVLIGEVRDHETAQ
                     IAIEAALTGHLVLTTLHTNNAPSAITRLIEMGIEPFLVGTAVDCVLAQRLARRLCSKC
                     KEAYVPTAEALVLARYPQSPDEPLPTLYRPIGCA"
BASE COUNT          919 a         1903 c         1879 g         1040 t
ORIGIN      
        1 gcccatggca tcggcgttgc cggccgggat ggacgaagac cgagtggccg ccatgagcgc
       61 ggcgctcttg tcgctcgccg aacgggccgc ccaggggctt ggccgtggcg atctttccca
      121 ggtctacatc gaaggcgaga acggcaccgt gttcctcgtc tcggcccagg acgaagccgt
      181 cctcatcgcg gtcaccgcgg ccgacaccaa ggtcgggatg atgctctacg aagtgaaact
      241 ggccgcgaac aaggtcggcg aggtccttcg cgggcccgcc cccctccccg cgccgctgcc
      301 gtcacacctc acccgcagct acctcgacga ggccgccgca gagactgatt cagccgcgta
      361 cgaaccggcc gaaccggcgt ccgacgaact gccttacggc gaagccgacc acagccaacc
      421 ctcatacgag cagccggcgt atgtcgaccc ggcgtatgaa gcagccgtcg aggccgccga
      481 agcgtccgag cccacacacg acgagcctgc gtatagcgag cccgtttaca cctccacgag
      541 taacgagtct gcggacgtac ccgccgaatc cgacgaggat gagtacgccg aagtcgccta
      601 caccagcgcg tcggctctca ccgagcctta cttcgctgaa gcacctgcca cgcccgcacc
      661 gcccgctgat ccgccggggt ggtcgtcgta cgcgccgagc ggcagcgtgc tccctggtgg
      721 acagggttcc tggtcttaag aggtagtccc cccgcgcacc gcaaccgtca acgctgcatc
      781 actatgcgac tgcccgcaac cggagaggta gaccagtgaa actcgaaggc tcacttgatg
      841 ccttcagtct tcctgacatc ttccagctgt tgagctttac gaagaagtcg ggggggttgc
      901 acctgcgtcg tgggaacgtc cacggcacgg tgtacttccg tgacggctcg gtcaccggtg
      961 cttcctccga tggtggtcgg caagcgctcg cgcgtcgcct cgtcgggagc ggcgcggtag
     1021 gcgacgacga actctcggcc gcggtcgagc gggccgcgaa tgacggggtc ggagtcggtc
     1081 gggcgctgct cgacgccgga gccgtcgacg ccgatctcct gcgaaacctg gtcgccgagc
     1141 aggcggtcga tgccgtcttc gacctgttgc gctggagcga gggcgacttc tccttcaccg
     1201 tcgatgagcc tggccccgac gacgtcgggt tctcgctcgg agtcgaaccg atcgtcaacg
     1261 acgcccgcag tcggctcgag acgtgggaac acgcctgccg ggtcatcccc tcacccgaga
     1321 cggtggtggc tctgccggtg gcggtccgcg aagacccgca gatctcgcgc ggcgaatggg
     1381 cgttgctcgc gctcgtagat ggacgccgca gtgttgccca actggtcgct ctcgcaggtc
     1441 gtggtgacta ctccgtggtt tctcaactgg ccgcgctgat cgagcgcgga ctgcttgcgg
     1501 cccgcacgcc agacgacgct gagggcgtac tcgcgctggc gcgacgccag gcgatcctcg
     1561 gtcggttgga gggcgacggg tcggccgctg ctgtggttgt ggaccttccc gacactgccg
     1621 atgtgcccag taccgccgat gcggtgccgg cgactgtctt cgccccgctg ccggcgactt
     1681 tcggcgctga ggccgctgag tttgtcgcac tcggggaagc tgtcgacagc gctgacaccg
     1741 tcgatgcgaa tgggaatctt gccggcgcgg ggctcgctga ggtctcggcg gcatcgaccg
     1801 tggcgtcggt actcgcggct gcaaacggcg agtcgtctcc ctttgcccgc acgcagacac
     1861 ctagttcgct gcgctccgcc gacgacgttc gcgcgcccaa cgccgaacgc gtccccgctg
     1921 ccgagcggcc tgccgcccgg gtcgaggagc gcatcgcggc ccacttggtt gccgacacgc
     1981 gcacgccagt gactccgtcg cggcccgagc cgttcatgcc caagcgccgc ccggagtttc
     2041 ctgacgacag ctcgcattct gcagcggccc gcatgaacgg cggcggcctc gcaacggcga
     2101 cggctgccgt ggcggatccg tttggtagca cgtcgcagat cgagcgcgat cccagcgtga
     2161 acaagagcct gttgctccgt ctcatcgccg gcgttcgggg gttgtgacat ggccatcaaa
     2221 cgcggtcgca gaggcggcaa cgcccaggcg gtgaagatcg tcgtcaccgg cccgttctcg
     2281 gcgggcaaga cgactctgat ccgcacgatc agcgagatca ccgtgctctc gacggagaaa
     2341 gacatcaccg acgagacgag gtcgaccaag gccgagacga cggtggccat ggacttcggc
     2401 cgcattacca tcgaccacga tctggtgctc tacctgttcg gtactcctgg ccaggaccgc
     2461 ttcgacttca tgtgggagat cctgggcgaa ggaatgctcg gctacgtgct gctcgtcgat
     2521 gctcagcgcc ccgaatcgct gtcagaagcg gtcgcgatcc tggcagcgtt caagaagatg
     2581 gcgcgggtgc cgttcgtcgt cggtctgaac cgggcacagg gcgccgaccc ggaggtcgag
     2641 agcaatgttc gtgcggtact cggactcgac gattcggtcc cggtcgtgcc gtgcgacgcg
     2701 accgacaggc aatcggtgaa ggacgtcttg ctggcgttgc tctacgccgt cgtcgactct
     2761 ctcgacgtgg cagccgccga agcataaggc gtcaaggcag gcggtaacgc tgccgatgga
     2821 cgtctcatga ccgcacttgc tgctcctcct gtgagcagtc gcagtccggt tacggcaccg
     2881 gtccagaagg caatcattcc gggaattctt gtcggacttg ccgtcgccta cgtcacgatg
     2941 ccttttgccc acgcgacggg tggccgggag ccctgggcct tgaccaaggc ggccatcgtc
     3001 gtcggcgtgg ctctcatcgc ggtcaagccg tggcgtgagc tcagcgcccg tgtgcttggt
     3061 ctcgctgtcg cggtgtcgct cgcggcgctt gcggtctgtc tcgtgacgcc tcctggctgg
     3121 ttcgacggca cccgcgcggc gagttatgcg ttggctgccg caacattcgt cacagtcgcg
     3181 gcgtacgcac gcagtgtgcg ccggtcgtac ccgatcgctg cgatcgtcgc gacggcgggt
     3241 gcggtgcagt tctgctggtc gttcgtcgcg tggtggggcg ggcgcgatca aagcatcccg
     3301 atggtcggaa ccttctactg gcacaaccag ttcgccgcgt tcctgcttgc gccagcgctg
     3361 atcgcactcg ccctgcttgt cggacgccgt gccccgtggc gactcgtggg ctgggttgtg
     3421 gcaccgcttg ccgtcgccgg agtcgttcac tcgtcgagca gaggcggcat gggggtgctg
     3481 ctcgcaggct ggctcgtgat cggcgtcctc gcggtgcgcg tccggccgaa ccgccgggcc
     3541 ttcgtcaaat ggttggcggc atcggtcctt gctgcgggcg tgacagtcgc tgtctcgagc
     3601 ccgcccttct tctcatcgac ggcctcaccg ttgtcggcca cccaggctcg cgcgagcacg
     3661 ggtgagaccg ctgacgccaa cggcgcatac cgcgtccaga tgtggcgcga gtcgatcacg
     3721 gtcttcgaac atcacccggt cacaggcgtc ggatacggcg gattgttgtc cgccgcgagc
     3781 aaactgacgc ccgccacatg ggcacggtcg ccgcttgcgc acgacgacta ccttcaggcc
     3841 ctcgctgagg gcggcttgct gctcggtctg ccgtttctag gcgcctgcgg ggtgatcgcc
     3901 atcgggctgt tgcgcgtcgg aagcagtcgt gtccacagcc gcgtcatcga cgtccgcact
     3961 ggtgttgtcg tcgcagcgat cgcactgatg gcgcacgctg cgatcgactt cgattggacc
     4021 tatcccgccc tcttctgtct cgcagccgtt gtgaccgcgt tggcagtcgg gccggtgctt
     4081 cggcgacccc ctaggtcggt tggggccaca agctctgatg ccgtcggttt gccggcacga
     4141 ccggttgtgc cgtgggctcg ggtggcattg gttggcctgc tcgttggcgc gactgtcgcc
     4201 ggcggtattg ccgagcaccg cggcgggctc cagatcgctt acaacgtcac gaacgcgaat
     4261 ccggcgtcgg catcagggac ggggcacggg tgaagcaact cggggacatc ctcttggaga
     4321 acggccttgt cagcggcaca cagctggcga cggcgtatga agaacatcaa cgcagcgggc
     4381 gcagtctcgg gcgtgttctt gtcgagcaag gtgtgctctc agaaggccaa ctcgttgctg
     4441 cactcgctac gcagatcggg ttgcgcttcg tcgacctcac cgagttctcc gtcgacggat
     4501 cggccgtttc ccgggtgcct gaagcagtct gtcggcgtta caccgcgctg ccggtcggct
     4561 atgacgacgg caagcttctg gtcgcgatgg ccgatcccgc caacgtattt gcgatcgacg
     4621 acatccgttc gataaccggc ctcgacgtca agccggcggt tgctacgcga gctgatgtgc
     4681 tcagcgcgat caaccgctac caccgagctg acggggaact cgacgatctg acgttgtcgt
     4741 tgagcgccgg cgacgacgat ctcgacgagc tcagcaacgt gaaggatctg gtcgaggacg
     4801 cgccgatcgt caagttcgtg aacctgctga tttcccaggc tgttcaagac cgtgcgtcgg
     4861 acatccatat cgagccgacc gagcgcgacc tgcgggtgag gtaccgcatc gacggcgtgc
     4921 tccatgaggt catgaagtcg ccgaagagca tccaggccgg cgtgacgagc cggctcaaga
     4981 tcatggccga gatgaacatc gccgaacggc ggattccgca agacggcagg ctttccgtga
     5041 acgtgacggg gaagaagatc gacctgcgcg tggcatcgct gccgacggtg tggggcgaga
     5101 agatcgtcat gcgaatcctg gacaactcga ccgcgctgct gaagctcacc gacctcggct
     5161 tctctgacgg caactacgaa cgctggtctc agagcttcac caagccctac ggcatgatgc
     5221 tcgtgaccgg cccgacaggc tcgggaaaat cgacgacgct atacgccacg ctcaatatcg
     5281 tctcgacccc cgacaagaac gtcatcaccg tcgaagaccc tgtcgagtac cggatccctg
     5341 gcatcaacca ggttcagacg aacgcgaagg ccgggctgac cttcgcgtcg gcgttacgct
     5401 cgatcctgcg gtctgaccct gacgtcgtgc ttatcggtga ggttcgcgac cacgaaacgg
     5461 cgcagatcgc gatcgaggcc gccctcactg gccacctggt cttgacaact ctgcacacga
     5521 acaacgctcc tagcgccatc acccgtctca tcgagatggg gatcgagcct ttcctcgtgg
     5581 gcaccgctgt cgactgtgtg ctcgcacagc gactggcgcg ccgcctttgt tcgaagtgca
     5641 aggaggcgta cgtgccgacc gccgaggccc tcgtgctcgc gcggtatccg cagtcgccag
     5701 acgagccgct gccgacgctg tatcggccca tcggatgcgc t
//
LOCUS       DASWVF010000716         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_376571, whole genome shotgun sequence.
ACCESSION   DASWVF010000716 DASWVF010000000
VERSION     DASWVF010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376571"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGD55_12660"
     CDS             <1..381
                     /locus_tag="VGD55_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0871244.1"
                     /translation="WRYTGLDFVLFLGAIQSIPGEFYEAAEIDGASRWQQFRFIIAPG
                     IKPITSLLTILAISGSISVFEVPFIMTGGSDGTSTFVIQTFNQAFQFQKVGLASAAAV
                     VLLAIVLIVTWIQRLLVPDEKDFA"
     gene            378..1277
                     /locus_tag="VGD55_12665"
     CDS             378..1277
                     /locus_tag="VGD55_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0871245.1"
                     /translation="MSAVTAGLRGDRSGQPSRQPTVRRLRSSTAVTLKYLTLILACVV
                     VLLPMIAVLMTALKTAKEVANDGSALTPPHNWFNYRNFVTAFNKGDMLQGLFNTGFLL
                     VFSVTGTVLIGSMTAYAIGRFNFRFKRVVVALFLLAALVPSVTTQVATFKVINFFGLV
                     DTRWGPILLYTGTDIVSIYIFLQFIRSIPISLDESARLDGASSFTIYRKIIFPLLKPA
                     IATVVIIKGIFVYNDFVSPFLYMPDSNLGTVSTSLFRFKGPFGAHWEDISAGAILVVV
                     PSLVIFLLLQKFIYNGLTRGAVK"
     gene            1289..3727
                     /locus_tag="VGD55_12670"
     CDS             1289..3727
                     /locus_tag="VGD55_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871246.1"
                     /translation="MQVPSHWTVTAVGGDVPDRIAGLVFPATVPGCVHLDLLDAQLIP
                     DPYLDENETALAWVGRVDWRYETTIDWDGTGSQVDLVALGLDTVATVELNGAVIAHTE
                     NMHRSYRFPVRDNLLVGANTLAITFAAGLTAAECASELLGPRPHVNAHPFNAIRKMAC
                     NFGWDWGPDLVTAGIWRPLFLETWARARIAGVRPLVSVEGSDGVLRAYVDIERAAGFD
                     EPVSLDVNVAGVRVRASVPAGVTSAMVRLVVPAVALWWPHGYGDQPLYGVNVVLSEGD
                     GGGELASWSSRVGFRTVTLDTAPDEQGTRFAIVVNGVPVFARGVNWIPDDAFPVRITR
                     ERYAQRLGQARDANVNLVRVWGGGIYESDDFYEVCDELGLLVWQDFLFACAAYAEEQP
                     LRGEVIAEAREAVTRLSQHPSLALWNGNNENIWGYEDWGWKEGIGDRTWGLGYYTEIL
                     PAIVAELDPTRPYTPGSPYSFSAGKHPNDPAHGTMHIWDVWNELDYTAYRRYVPRFVA
                     EFGFQGPPTWATLDRAVHDDPLTPTSPGVLLHQKADDGNGKLARGLDTHLPPPRSVDE
                     WHWATSLNQARAVSLGVEHFRSWSPVCMGTIVWQLNDTWPAVSWAAVDGDGRRKPLWY
                     GLRRSYRDRLLTFQPRDGGLTLVAVNDSAAPWRGDVMLSRRASDGVVMSLATLALDVG
                     ARRTVAFAVPGEVAASDGGVGGFILAESGAERAWWHTEEDVDAKLPAPELRCALEPVA
                     GGYRLSVTAHTLIRDLALLVDRVAEDAVVDDMLVTLLPGETVVFTIATAAPMTLEQLT
                     DPLVLRSANQLVAD"
BASE COUNT          612 a         1191 c         1242 g          765 t
ORIGIN      
        1 tggcgttaca cagggctcga tttcgtgctg tttctcggtg cgatccagtc gatcccgggc
       61 gagttctacg aggcggccga gattgacgga gccagccggt ggcaacagtt ccgtttcatc
      121 atcgcgcctg gcatcaagcc gatcaccagc ttgctcacca tcttggcgat ctccgggtcg
      181 atctcagtct tcgaggtgcc gttcatcatg accggcggat cggatggcac gtcgacgttt
      241 gtcatccaga ccttcaatca ggcgttccag ttccagaagg tagggctcgc ctctgctgcc
      301 gcggtcgtct tgctggcaat cgttttgatc gtgacgtgga ttcagcgcct gctcgtgccg
      361 gacgagaagg acttcgcatg agcgccgtga cagcgggtct gcggggtgac cgatccggtc
      421 agccgagccg gcagccaacg gtccgccggt tgcggtcgtc aacggccgtc accttgaagt
      481 acctcacgtt gatcctggcc tgcgttgtcg tgctgctgcc gatgatcgcg gtcttgatga
      541 cggcgttgaa gaccgcgaag gaggttgcga acgacggctc ggcgttgacg ccgccgcaca
      601 actggttcaa ctaccgcaac ttcgtgactg ccttcaacaa aggcgacatg cttcagggtc
      661 tgttcaacac cggtttcctg ctggtctttt cggtgactgg cacggtcttg atcggatcga
      721 tgacggcgta cgcgatcggc cgcttcaact tccgattcaa gcgcgtggtc gtcgcgttgt
      781 tcctgctggc cgcactcgtg ccgagtgtga ccactcaggt ggcgaccttc aaggtgatca
      841 acttcttcgg ccttgtcgac acgcgctggg gcccgatcct gctctatacc ggcaccgaca
      901 tcgtctcgat ctacatcttc ttgcagttca tccggagcat cccgatctca ctcgatgagt
      961 cggcgcggct ggacggcgcg agctcgttca cgatctaccg caagatcatc tttccgttgc
     1021 tgaagcccgc tatcgcgacg gtcgtcatca tcaagggaat cttcgtctac aacgacttcg
     1081 tgagcccgtt cctgtatatg cccgactcga acctcggcac ggtgtcgacg tcgttgttcc
     1141 ggttcaaggg accgttcggt gcgcactggg aagacatttc cgccggcgcg attctggtcg
     1201 tcgtcccatc gctcgtcatc ttcttgctgc ttcaaaagtt catctacaac ggcctcaccc
     1261 ggggcgcagt gaaatgagga accaccgcat gcaggtgccc agccactgga cagtgaccgc
     1321 cgtcggtggc gatgtcccgg atcggatcgc tggcctggtg tttcccgcga cagtgccggg
     1381 ttgcgtgcat ctcgacctgc tcgacgctca actgattcct gacccgtacc tagacgagaa
     1441 cgagacggcg ctcgcgtggg tggggcgtgt cgactggcgt tatgagacca cgatcgactg
     1501 ggacggcacc ggctcgcagg tcgacctggt cgcgctcggg ctcgacacgg tcgcgacggt
     1561 cgaactcaac ggagccgtga tcgcgcacac cgagaacatg caccgcagct atcgattccc
     1621 ggtacgcgac aacctgctcg taggtgccaa cacgcttgcc atcacgttcg ctgcgggcct
     1681 cacggcagcc gagtgcgcga gcgaacttct cggcccgcgg ccgcacgtca acgctcaccc
     1741 gttcaacgcg atacgcaaga tggcgtgcaa cttcggctgg gactggggcc ccgaccttgt
     1801 caccgcaggc atttggcggc cgttgttcct cgagacctgg gcacgggccc gcattgccgg
     1861 cgtccgcccg cttgtttctg ttgaggggtc ggacggcgtg ttgcgggcgt atgtcgatat
     1921 cgagcgggcg gcagggttcg acgaaccggt cagcctcgat gtgaacgttg cgggtgtgcg
     1981 ggtgcgcgcg tcggtacctg cgggtgtgac gtcggcaatg gtgcgcctcg tcgtcccagc
     2041 tgtcgcgttg tggtggccgc acggatacgg ggatcaaccg ctgtacgggg tgaacgtcgt
     2101 gctgtccgag ggcgacggcg gcggcgagtt ggctagctgg tcgtcgcggg ttggatttcg
     2161 caccgtcaca cttgatacgg cacccgacga gcagggcacc cgcttcgcga tcgtcgtgaa
     2221 cggcgtgcct gtcttcgcac gcggagtcaa ctggatcccc gacgacgcgt tcccggtgcg
     2281 catcacccgc gagcgctacg cgcaacggct cgggcaggcc cgcgacgcca acgtcaatct
     2341 cgttcgcgtc tggggaggtg gcatctacga aagcgacgac ttctacgaag tttgtgatga
     2401 gctcggattg cttgtctggc aggacttttt gttcgcctgc gccgcctatg cggaggagca
     2461 gccgctgcgg ggtgaggtga tcgccgaggc gcgcgaggcg gtgacccgct tgagccagca
     2521 tccgagcctt gcgttgtgga acggcaacaa cgagaacatc tggggctacg aggactgggg
     2581 ttggaaggag ggcatcggcg accgtacgtg gggcttgggg tactacaccg agatcctccc
     2641 ggccatcgtg gcggagctcg acccgacgag gccctacacg cctggcagtc cgtattcatt
     2701 ctccgcggga aagcacccca acgatcccgc ccacggcacg atgcatatct gggacgtctg
     2761 gaacgagctc gactacaccg cgtaccggcg ctacgttccg cgcttcgttg ccgagttcgg
     2821 atttcaaggg ccgcccacgt gggccactct cgaccgagca gttcacgacg acccacttac
     2881 gccgacgtca cccggtgtgc tgttgcatca gaaggccgac gacggcaacg gcaagctcgc
     2941 tcgcggcctc gacacccacc tgccaccgcc caggtcggtc gacgagtggc attgggccac
     3001 gtcgctgaat caggcgaggg ccgtgtcgct tggtgtcgaa cacttcaggt cgtggtcacc
     3061 ggtgtgcatg ggcacgatcg tgtggcagct caacgatacg tggcccgctg tttcctgggc
     3121 tgcggtcgac ggagacggac ggcgaaagcc gctctggtac gggttgcggc gtagctatcg
     3181 cgatcggttg ctgacgttcc agccgcgcga tggaggactc acgctcgtcg ccgtcaacga
     3241 ttctgctgcg ccgtggcgtg gtgacgtgat gttgagccgg cgggcgtccg acggcgtcgt
     3301 tatgtcgttg gcgacgttgg cactcgacgt cggtgcgcgc cgaacggtgg cgttcgccgt
     3361 tccgggcgaa gtagcggcat ccgatggcgg tgtgggcggg ttcatccttg ccgagagcgg
     3421 cgccgaacga gcgtggtggc acaccgaaga ggacgtcgac gccaagctgc ccgcgcccga
     3481 gctgaggtgc gcgctcgaac cggtcgccgg cggctaccgc ctcagcgtca cggctcacac
     3541 tttgattcgc gacctcgccc tgctggtcga tcgcgttgcc gaagacgcgg tcgtcgacga
     3601 catgttggtc accctcttgc ccggtgagac ggttgtcttc accatcgcaa cggcggcgcc
     3661 gatgacgctc gagcagttga ccgacccgct ggtgttgcgg tcggcaaatc agctggtggc
     3721 cgattgaccc gtggcgggtt gcagctcgac atagacaccc ccggccgcaa ccagctgatc
     3781 gtgcgtgccg atctcgacga tgcgcccggc
//
LOCUS       DASWVF010000717         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_300028, whole genome shotgun sequence.
ACCESSION   DASWVF010000717 DASWVF010000000
VERSION     DASWVF010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300028"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGD55_12675"
     CDS             complement(<1..619)
                     /locus_tag="VGD55_12675"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY0871247.1"
                     /translation="MASPLELFTDLCFVVAISQAASAFHAHLSQGHGGKGLLGFAMVF
                     FAIFWAWLNFSWFASAYDNDDVGYRLLTLLHVLGSLVLAAGIPGMVDGDFALGVLGYL
                     IMRSSLVVQWLRVAANDAPRRTTARRYATGIVVVQLCWVGFIFLPYSFEVPAFSVGVV
                     AELVVPAWAERAGITPWHPHHIAERYSLFYIIVLGETVLSTTVAIQ"
     gene            727..1044
                     /locus_tag="VGD55_12680"
     CDS             727..1044
                     /locus_tag="VGD55_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0871248.1"
                     /translation="MFALVVRFDLPDGASAREFDELTAAAVPLIHSHEPGTLVYETYA
                     VEGEPLARLFYEVYRDRGAHGEHERQPHTAKFLATIATLVAQTRVEYLSQVEPSARVE
                     HAT"
     gene            complement(1058..1864)
                     /locus_tag="VGD55_12685"
     CDS             complement(1058..1864)
                     /locus_tag="VGD55_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871249.1"
                     /translation="MTPPTGRAALVLRHGRTLGVVVLAVSLGLGLSGCSAIRKVKAVA
                     ADVKNNKKTMDSFTTQIQSGEAKPFEVTYVTTGSTPTTVVYAVQPPAGLSFTNTPSST
                     SSAGAGSAAANSAPSLDIIVNSTGEYSCTPPDTSGTAGKGTWACQMLSPGDAASQNAI
                     FDFYTPAHWVNFLKGFSIAAGFAGDQVTSSTMTVNGFAMSCVDFKAPGDPGESTICTT
                     AQGILGYVKVADDATSFEIKSYTASPPASLFALPPGATVTKVQVASSPAA"
     gene            complement(2218..>2505)
                     /locus_tag="VGD55_12690"
     CDS             complement(2218..>2505)
                     /locus_tag="VGD55_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871250.1"
                     /translation="IQSMPPYDMWLAGRDDILKWFFGPGIGCQGSRVIPTLPANGSVA
                     FGQYKPSADGGYEPWALQVIELRDGMIGELTFFLSTQTLFPLFGLPLEFEG"
BASE COUNT          481 a          816 c          804 g          404 t
ORIGIN      
        1 gctggatcgc gacggtcgtc gacagcaccg tctcaccgag cacgatgatg tagaacaggc
       61 tgtaccgctc ggctatgtga tgcgggtgcc acggcgtaat gccggctcgc tcggcccacg
      121 ctggcaccac cagctcggcc actaccccga cgctgaaggc aggaacctcg aacgagtacg
      181 gcaaaaagat gaagccgacc caacacagtt ggacgacgac gatgccggtc gcgtatcggc
      241 gtgccgtcgt acgacgcggc gcatcgttgg ccgcgacgcg caaccactgc acgaccagcg
      301 atgaacgcat gatgaggtaa cccaagacac ctagtgcgaa gtcgccgtcg accatgccgg
      361 gaatcccggc cgcaagcacg agggagccga gcacgtgcag cagcgtcagc agccgataac
      421 cgacatcatc gttgtcgtag gccgacgcga accaggagaa gttgagccac gcccagaaga
      481 tggcgaagaa caccatcgca aatccgagca atccctttcc gccgtgaccc tgagacaggt
      541 gtgcgtggaa cgcggacgcc gcttgcgaga tcgccacgac gaagcagagg tcggtgaaca
      601 actccaatgg tgaagccacc cggtgcgcct ccgccgggtc gcgaggcacc aacgggttga
      661 tgaaacgact ggcagcgcgg ggagtcacat cggaaccata gagccgcctg cgctagcgtg
      721 ccgaccatgt tcgccttggt ggtcaggttc gatctgcccg acggtgcttc cgcgcgcgag
      781 ttcgacgaac tgaccgcagc cgcagtgccg ttgattcatt cgcacgagcc aggaacgctc
      841 gtctacgaga cgtacgccgt cgaaggtgaa cccctcgcgc ggttgttcta cgaggtctat
      901 cgcgaccgtg gcgcacacgg cgagcacgaa cgccagccgc acaccgcgaa gttcctggct
      961 accatcgcca cgctggtcgc gcagacccgc gtcgagtacc tctcgcaggt cgagccatca
     1021 gcacgggtcg agcacgcaac ctgacctagc tcttggccta ggcggccggc gacgacgcga
     1081 cctgaacctt ggtgacagtg gctccgggcg gcagcgcgaa cagtgacgcg ggcggcgacg
     1141 ccgtgtagct cttgatttca aaactcgtgg cgtcgtcggc caccttcacg tagcccaaga
     1201 tgccctgcgc cgtcgtgcag atcgtgcttt caccgggatc gccaggcgcc ttgaagtcga
     1261 cgcacgacat cgcgaacccg ttcacggtca tggtcgacga ggtgacctgg tcgccggcga
     1321 agccggcggc gatcgagaag cccttgagga agttgaccca gtgcgcgggt gtgtagaagt
     1381 cgaagatggc gttctgcgag gcggcgtcgc ctggcgacaa catctggcac gcccacgtac
     1441 ccttaccggc cgtgcccgac gtgtcgggcg gcgtgcagga gtactcaccc gtcgagttca
     1501 cgatgatgtc gagactcggt gcgctgttcg cggccgcgct gcccgcgcct gcgctgctgg
     1561 tgctgctggg cgtgttcgta aaagacaggc ccgctggcgg ctggacggcg tacacgaccg
     1621 ttgtcggcgt cgagccggtc gtcacgtagg tgacctcgaa cggcttggct tctccagact
     1681 ggatctgggt cgtgaaggag tccatcgtct tcttgttgtt cttgacgtca gccgcgacgg
     1741 ccttgacctt cctgatggcc gagcatccgc tgagacccaa gcccagggag acggcgagca
     1801 cgacaacgcc gagggtccgg ccgtgccgaa ggacgagggc tgcgcgaccg gtgggtgggg
     1861 tcatgtctgg gcctccgatg ctggcacgcg aacgcgtact cccgagttct ctcgcggaga
     1921 atacggcctc cgccgccggc ccggcaccgt ctcaccgatg aattacaccg cctcaccgat
     1981 gaattacaga gatggccgtc gtctgtctac agatggttac gtctgcgccg cgcccgacgg
     2041 agcgcgtggt catcgactgg gacgtctccg gcccgccgat tgacgtggct acggtcgaga
     2101 cgctcgcgcg atgccagctg gccgctcgac ggcacgggtt cacgctgcga ctgcgcaatg
     2161 tggcagcgga actggcagag ctgatcgggt tgctcggcct aaacgacgcc ctcgcaatca
     2221 gccctcgaac tccagcggca ggccgaacag cgggaacaac gtctgcgtag acaggaagaa
     2281 cgtcagttca ccgatcatgc cgtcgcgcag ttcgatgacc tggagagccc acggctcgta
     2341 tccgccgtcc gcactcggct tgtactgccc gaacgccacc gaaccattgg ccggcagcgt
     2401 cggaatgacc cgcgagccct gacagccgat cccgggcccg aagaaccact tcagaatgtc
     2461 gtcacgacct gccagccaca tgtcgtacgg cggcatcgac tggat
//
LOCUS       DASWVF010000718         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_45289, whole genome shotgun sequence.
ACCESSION   DASWVF010000718 DASWVF010000000
VERSION     DASWVF010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45289"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VGD55_12695"
     CDS             complement(<1..1075)
                     /locus_tag="VGD55_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871251.1"
                     /translation="MTGSAFVAATYALVDRNLTSSAATAQTANQTQDDTVLAKICEKE
                     KVGGQLTDAQIAQCKTAFAGGAQAGAANQRNRDLHQQVLWSIFGLIGGTIVAGVLGWV
                     IGRRILTPLHKVTSAAQRASQEHLDERIHHDGPNDELKKLADTFDDMLDRLDSAFASQ
                     RRFVANASHELRTPLTSMRTLIDVAMAKPTRTTEGLETLVGRVREAVDQSEAIIDGLL
                     TLARSDRGLTVRELVDLEAAALDAIDQTSAAARDSDITVEANLLPALIPGDRVLVERL
                     VANLVDNAIRYNFEGGSIQVETGTRDGQSYLSVTNSGPLVQESKLASLFEPFTRLEQR
                     VSNGQGVGLGLSIVASVVAAHGGR"
     gene            complement(1165..1818)
                     /locus_tag="VGD55_12700"
     CDS             complement(1165..1818)
                     /locus_tag="VGD55_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0871252.1"
                     /translation="MRVLVVEDNAVVADAIVEGLRDQGMAVDVAYDGSSGLEKVTVNE
                     YEVVVLDRDLPRLHGDELCATIVNSPCTSRILMLTAAGQVDDRVEGLNLGADDYLAKP
                     FDFSELVARVRALARRSPSAPPVLTRGDLSVDRVRRAVTRSGRPVPLARKELGVLEVL
                     ITADGGVVSSEQLLEQVWDENADPFSNTVAVTVLRLRRKLGEPPVIDTVIGGGYRIS"
     gene            1912..2499
                     /locus_tag="VGD55_12705"
     CDS             1912..2499
                     /locus_tag="VGD55_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871253.1"
                     /translation="MNQLIGRRAVLAVALLAPITLLATACTSGSHSASVARLSAATTT
                     ASSSSSSAAASDLATQALAFARCLRANGLPNWPDPLSNGTFDKSKLQATGYSTAQTRA
                     VEDGPCKSILPTTAPSSPNSQTITTQQQQDYINAVACMRAHGFSNFPDPTFSGDHVTL
                     TPPPGVDTNSTQFTQAQHICKQLIPAGLPYSGSDA"
     gene            2496..3014
                     /locus_tag="VGD55_12710"
     CDS             2496..3014
                     /locus_tag="VGD55_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871254.1"
                     /translation="MTYIRVLAAGGLFLLLAACSGSSSPAASSTQQAGAVGFAHCMRS
                     SGVANWPDPESSGVFDKSEITTQKLGVSSSVLQSAQNTCQHLLPRESSTQQRLNGAQA
                     LQFAQCMRSHGVPNFPDPDGTGRIPDPATGGIDQGSPQFQAANDACVQYRPPYFPSNQ
                     AYAAYAATASAS"
BASE COUNT          540 a          999 c          965 g          529 t
ORIGIN      
        1 ggcggccacc gtgagccgcc acgacggacg cgacgatcga cagcccgaga cccacgccct
       61 gcccgttgct gacccgttgc tcgagacgcg tgaacggttc gaagagagat gcgagcttcg
      121 attcctgaac cagcggtccg ctgttggtca ccgagaggta cgactggccg tcgcgggtgc
      181 cggtctcgac ctggatcgat ccgccctcga agttgtagcg gatggcgttg tcgacgaggt
      241 tggcgacgag acgctcaacg aggacccggt cacccgggat gagggcaggt aacaggttcg
      301 cttcgacggt gatgtcggag tctcgtgcgg cggcgctggt ttggtcgatc gcatccagcg
      361 cggcggcttc gaggtcgacc agttcgcgga cagtgaggcc gcggtcggac cgagccaagg
      421 tgagcaagcc gtcgatgatt gcttccgatt gatccactgc ctcgcggact cggccgacaa
      481 gcgtttcgag tccctcggtg gttctggtgg gcttggccat ggcgacgtcg atgagggtgc
      541 gcatcgacgt gagcggagtc cggagctcgt gcgaggcgtt ggcgacgaat cgccgctggc
      601 ttgcgaacgc ggaatcaagg cgatccagca tgtcgtcgaa ggtgtcggcg agtttcttga
      661 gctcgtcgtt gggtccgtca tgatggatgc gctcatcgag atgttcttgc gaggcgcgtt
      721 gtgcggcgct ggtgacttta tgaagcgggg tcaggatccg ccggccgatc acccatccca
      781 atacgcccgc aacgatagtg ccgccaatga gaccgaagat ggaccacagc acttgctgat
      841 gaaggtcgcg gtttcgctgg ttagcggcgc cggcttgggc acccccggcg aaggcggtct
      901 tgcactgggc aatctgggcg tcggtgagtt ggccgccgac cttctctttc tcgcaaatct
      961 tggcgagcac tgtgtcgtcc tgcgtttggt tggcagtttg ggccgtggcc gcgctcgacg
     1021 tgaggttgcg gtcgacgagg gcgtacgtcg cggccacgaa cgccgagccg gtcaccagaa
     1081 acagtccggc gcaggcgagt gcgatccggg cccgcacgct gcgtcggccg aggaatctgc
     1141 gtcgagttcc gagggggggc atctctagga gatccggtat ccgccaccga tgaccgtgtc
     1201 gatcacggga ggttccccga gttttctgcg aaggcgcagc acggtgaccg cgacggtgtt
     1261 gctgaacgga tcggcgttct cgtcccagac ctgctcgagc agctgctcag agctcaccac
     1321 cccgccgtcc gccgttatca gcacttcgag caccccgagc tccttgcgcg ccaggggtac
     1381 gggacgcccc gacctcgtca cggctctgcg aacgcgatca acgctcagat caccgcgggt
     1441 cagcacgggc ggcgccgacg gactgcggcg ggcaagggct cgcactcggg ccaccaattc
     1501 ggagaagtcg aatggcttcg ccaaatagtc gtcggcgccg aggttcaagc cctccacgcg
     1561 gtcgtcgact tggcccgcag cagtcagcat cagaatccgg ctcgtgcacg gcgagttgac
     1621 gatcgtcgcg cagagctcgt cgccgtgcag tcggggaagg tcgcgatcta gaacgaccac
     1681 ctcgtactca ttcaccgtca ctttctccag accgctcgac ccgtcgtagg cgacatcgac
     1741 tgccatcccc tggtcgcgca gtccctcgac gatggcgtcc gcgaccaccg cgttgtcttc
     1801 gacgaccagc accctcatgc ctccatggtg gcgcacctcg ggtgacaccg cgataacacg
     1861 catcagctgg tgttatccca gtgtcaccgg ctccctcgta gcgtcgcctt catgaatcaa
     1921 ctcatcggac ggcgagctgt gctcgccgta gcgctactgg cacctatcac cctgctggcg
     1981 accgcgtgca caagcggctc acacagtgct tcggtagctc ggctcagtgc agcgacaacc
     2041 accgccagct cttcgtcgag ctcggcggcc gcctcggacc tcgcgaccca ggcgctcgcg
     2101 ttcgcgcgct gcctgcgcgc caacgggctg ccgaactggc ccgacccgct gagcaacggg
     2161 acgttcgata agtcgaagct gcaggcgacc gggtacagca ccgcgcagac gagggccgtc
     2221 gaggacggcc cctgcaagag cattctccca actacagcac cgagttcgcc gaacagccaa
     2281 acgatcacaa cccaacaaca gcaggactac atcaacgcag tcgcctgcat gcgcgcacac
     2341 gggttctcga acttccccga cccgacgttc tccggcgacc acgtgaccct gactcctccg
     2401 cccggcgtcg acacgaactc aacacagttc acgcaggccc aacacatctg caagcagctc
     2461 atccctgccg gccttccata tagcgggtca gacgcgtgac gtacatcaga gtgctcgccg
     2521 ccggagggct tttcctattg ctggcagcgt gcagcggtag ctcgtcacca gcggcatcgt
     2581 caacgcagca agccggtgcc gttgggtttg cccactgcat gcgatcgagc ggcgtggcga
     2641 actggcccga cccagagagc agcggggtat tcgacaagtc agagatcacc acgcagaagc
     2701 ttggcgtgag cagctcggtg ctccagtcgg cacagaacac ctgccagcat ctgctgccgc
     2761 gcgagtccag cactcagcag cgactcaatg gggcgcaggc acttcagttc gcgcagtgca
     2821 tgcgcagtca tggcgtgccg aacttcccgg atccggacgg taccggccgc attcccgacc
     2881 cagccactgg tggaatcgat cagggttcac cgcagtttca ggccgcgaac gacgcctgcg
     2941 tgcagtaccg gccgccgtac tttccttcca accaggccta tgccgcgtac gccgcgactg
     3001 cgagcgcgtc gtgagcgatc gaacgaaggc gga
//
LOCUS       DASWVF010000719         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_402341, whole genome shotgun sequence.
ACCESSION   DASWVF010000719 DASWVF010000000
VERSION     DASWVF010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402341"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VGD55_12715"
     CDS             complement(<1..960)
                     /locus_tag="VGD55_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0871255.1"
                     /translation="MFTPMPNQVDLPALEHDVLRRWRESKVFERSLAATRGRPQWTFY
                     EGPPTANGMPGTHHVEARVFKDLFPRFKTMKGYHVPRKAGWDCHGLPVELQVERELGL
                     NGKKDIEEYGIAEFNARCRASVERHVDEFEKLTERMGYWVDMSKAYRTMDASYIESVW
                     WSLKAVFDKGLLVSDYRVAPYCPRCGTALSDHEVAQGYLNVTDPSVYVRFPVTSGPFA
                     GQADLLVWTTTPWTLVSNTAVAVHPDVEYDIVSLSATDDAPAERLVIAKPLVSKVVGD
                     RPHEILRSFSGRELEHWSYQRPFELVDFDGERAHYVVLADYVTT"
     gene            complement(1222..>1585)
                     /locus_tag="VGD55_12720"
     CDS             complement(1222..>1585)
                     /locus_tag="VGD55_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0871256.1"
                     /translation="QLKAAGVRLVALSNWSAETFPRVRHRLGFLDDFEGVLVSGTVGL
                     IKPDPAIYLLLIERHAVDVRHSMFVDDSERNVQAAAELGFDAIHFQSSEQLGEELRRR
                     GLITPVDSEAAEGVEGDE"
BASE COUNT          273 a          517 c          502 g          293 t
ORIGIN      
        1 ggtggtgacg tagtcagcca agacgacgta gtgcgcacgc tcgccatcga agtcgacgag
       61 ttcgaagggt cgctggtacg accagtgctc gagttcgcgt ccgctgaaac tgcgcaggat
      121 ctcgtgtggt cgatcgccga caaccttgct caccaacggc tttgcgatca ccaaccgttc
      181 ggcgggcgcg tcatcggtgg ccgacaagct gacgatgtcg tactcgacgt cgggatgaac
      241 cgcgacagcg gtgttgctga ctaacgtcca gggcgtcgtc gtccagacca gcaggtcggc
      301 ttgcccggcg aacgggccgg aggtcacggg aaagcgcacg tagacgctgg ggtccgtgac
      361 gttgagatat ccctgggcga cctcgtgatc tgaaagggcg gtcccacagc gcgggcagta
      421 cggcgcgacc cggtagtccg acacgagcag gcccttgtcg aagacggcct tgagcgacca
      481 ccagacgctt tcgatgtaac tcgcgtccat cgtgcggtag gccttggaca tgtcgaccca
      541 gtagcccatg cgctcggtga gcttttcgaa ctcgtcgacg tgacgttcga cagatgcccg
      601 acacctggcg ttgaactcgg cgatgccgta ttcctcgata tccttcttgc cgttgagccc
      661 gagctctctc tcgacttgga gttcaaccgg cagaccgtgg cagtcccagc ccgctttgcg
      721 cgggacgtgg taacccttca tggtcttgaa ccgggggaac aggtccttga agacccgagc
      781 ctcgacgtgg tgggtgcccg gcatgccgtt ggccgtcggc ggtccttcgt agaacgtcca
      841 ctgcggtcgg ccccgcgtcg cggctagcga gcgctcgaaa accttcgact ctcgccagcg
      901 ccgcagcaca tcgtgttcga gtgctggcag gtcgacctgg ttgggcatcg gggtgaacat
      961 caagcctccg tcgtcggcgc acccggtcgg tgcagtcttc ggcgaaggga cgagaccgtg
     1021 ccggtcgccc cggccgcgct cccgcggtac caccctcctt gggccgagga gatcttcagc
     1081 cccgctcttg ttccgcgacg ccgactctaa taggcgcgac caaaggccgc accgttctgc
     1141 cagcagctca ggggtgatct tcgtcgcgtg ctcagccccg ggctcacacc gtccccggat
     1201 cgctcgtgcc tgcgtgcgcg actactcgtc cccttcgacg ccttccgcgg cctccgagtc
     1261 tacgggagtg atcaacccgc gacggcgtag ttcttcgccg agctgctccg atgattggaa
     1321 atgaatcgca tcgaacccga gctccgccgc ggcttggacg ttgcgttccg agtcatcgac
     1381 aaacatcgaa tgacggacgt cgacggcgtg cctttcgatg agcagcaggt agatcgcagg
     1441 gtcaggtttg atgagtccga cggttccgga caccagcacc ccctcgaagt cgtcgaggaa
     1501 accaagacgg tgccggaccc tcgggaaggt ctcggccgac cagttcgaca gggcgaccaa
     1561 ccggaccccg gccgccttca gctgc
//
LOCUS       DASWVF010000720         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_402364, whole genome shotgun sequence.
ACCESSION   DASWVF010000720 DASWVF010000000
VERSION     DASWVF010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402364"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VGD55_12725"
     CDS             complement(<1..1687)
                     /locus_tag="VGD55_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871257.1"
                     /translation="MSLSKTARGARVPEPADDARASTTPGRILALASVLPAVLAAAWV
                     AVALPLLALHVYTPLPGTLCALALAGVLARPAIRVALASADHFDDVSWWVLAAVLVIV
                     VAAGVLAFAHSAEDVLVRRDPGSYAMSATWLSSHGTIQMPTHSALFGTQNDNLVLASQ
                     GFYQQGSHIIPQFMTGVPVLLSIGGWISGVTGVLHGNAVLGSLALLAFAGVAVRLVGA
                     RWAPLAVLALAFVQPELDVMRATYSEPAAQLILLGGLAIVLDAFAAGGLVPGFWARTQ
                     DVEVPVTDPDTAAWGLFVGGLVLGLVFVVRIDAVADLFPLVPFVGWLAYHRIVGWRRF
                     AGGMLLGLAFGGFDCLVLTYPYTQHVGSDLATVGIGFAGLVPATVAGVFIAWNRHKST
                     NAAGTDEAPATDAARRPWPQAAAAIVFLAGLFFFLRPHLMTMRANPASGGANYVMQVQ
                     RFLHMAVDPTRSYYENATIWLSWYLGWGTLALALGGACWLAYEQMAGRRRVWAPAFLV
                     FFGTAFAVLLRPSITPDHPWADRRFVPVVLPGLVLFAIAAVPPVLRWISDRAPG"
     gene            complement(1684..>2797)
                     /locus_tag="VGD55_12730"
     CDS             complement(1684..>2797)
                     /locus_tag="VGD55_12730"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0871258.1"
                     /translation="YLIAAAYALAWGLQAAMAADFHEIAFAVPLLAIAIERLDADRLR
                     TAVIAALLLLLVKEDFGIVVAAFGVVVGLRTQRWRLASVLAVVGVATTVLAERVLIPA
                     FGGKSGYYWDYYAALGSGPLQAAWHVVRHPLSTLHLMVTPHAKVRLLEWLFVPLGFMP
                     LGSPFVLLAVPLLAERLLSNNPNHWTLTHQYSAPFVPILTLAAVDTVARLRNALQART
                     STSGHRKQLGAPTWAIGPAYALCILVVAVWACGRMPFGQFRHGVPQSWRRTSYDQARQ
                     AAVGVVPSGVVVEASDLLAPQLVDRDQVILLDANPHSAPWVVFDAGNLEFPMTPDAQA
                     TRTSWLVSHGYAQVFSRDSILVYHRPTQAPLTATTS"
BASE COUNT          561 a          923 c          965 g          348 t
ORIGIN      
        1 atcccggcgc cctgtcggag atccagcgca agacgggcgg gaccgcggcg attgcgaaca
       61 gcacgagccc cggcaacacg acgggcacga aacgccggtc ggcccatggg tggtcgggag
      121 tgatgctcgg tcgcagcaag acagcaaacg ccgtgccgaa gaagacgagg aaagcaggcg
      181 cccacacccg tcggcgtccg gccatctgct cgtacgcgag ccagcacgca ccgccgagcg
      241 caagagcgag cgtgccccaa ccgaggtacc acgacaacca gatcgtcgcg ttctcgtagt
      301 aggacctcgt ggggtcgacc gccatgtgca ggaaccgctg cacctgcatc acgtagttcg
      361 ctccgccgga cgccgggttg gcgcgcatcg tcatgagatg gggacgcagg aagaagaaaa
      421 gtccagccaa gaacacgatg gcagcagcgg cttgtggcca cggccggcgg gccgcatcgg
      481 tcgcgggtgc ctcgtcggtg ccggcggcgt tcgtggattt gtgccggttc cacgcgatga
      541 acacgccggc caccgtcgcc ggcaccagac cggcgaaccc gatcccgacc gtggcgaggt
      601 cggagcccac gtgctgcgtg taggggtagg tcaggaccag gcagtcgaac ccgccgaacg
      661 cgaggccgag cagcatcccg ccggcgaaac ggcgccagcc cacgatccgg tgataggcca
      721 gccagccgac gaacggcacc agcgggaaca ggtcggcgac ggcgtcgatg cggacgacga
      781 acacgaggcc gagcacgagc ccgccgacga agagacccca cgcggccgtg tcggggtccg
      841 tcaccggcac ttcgacgtct tgcgtgcgtg cccagaaacc gggtaccagg ccgcccgcgg
      901 cgaacgcgtc gagcacgatc gcgagcccgc cgagcaggat caactgcgcc gccggttcgc
      961 tgtaggtggc gcgcatcacg tcgagttcgg gctgcacgaa ggccaacgcg agcaccgcga
     1021 gtggcgccca ccgcgcgccg acgagtcgga cggcgacccc ggcgaacgca agcagcgcga
     1081 gggagccgag aaccgcgttg ccgtgcagca cgccggtgac tccggagatc cagccgccga
     1141 tcgatagcag taccggcact ccggtcatga actgcggaat gatgtgcgag ccctgctggt
     1201 agaaaccctg cgacgcgagc accaagttgt cgttctgggt gccgaacaag gcagagtgcg
     1261 tcggcatctg aatcgtgccg tgactcgaga gccaggtggc gctcatcgcg tacgagccag
     1321 ggtcgcgtcg taccagcacg tcttctgcac tgtgtgcaaa cgccagcact ccagcagcaa
     1381 caacaatgac gagcacagcg gcgaggaccc accacgagac gtcgtcgaaa tggtcggcgc
     1441 tcgccaacgc aaccctgatg gccggccgcg cgaggacgcc ggcgagcgcg agcgcgcaaa
     1501 gcgtgccggg gagaggcgtg tacacatgca gcgcaagaag tggcaaggca accgctaccc
     1561 atgcggccgc cagtaccgcc ggcaagaccg aggcgagcgc gagaatgcgg cccggcgtgg
     1621 tcgacgcgcg cgcatcatcg gccggttcgg gtacccgcgc cccgcgcgcg gtcttcgaca
     1681 agctcatgag gttgtcgccg tcagcggagc ctgcgtcggc cggtggtaga ccaggatcga
     1741 gtcgcgcgag aacacctgcg cgtacccgtg cgacaccagc cacgaggtgc gagtggcctg
     1801 ggcatcgggc gtcatcggaa actccagatt ccccgcatcg aacacgaccc acggagcact
     1861 gtgcgggttc gcgtcgagca ggatcacctg gtcacgatcg accaattgcg gtgcgagtag
     1921 gtcgctcgcc tcgacgacga caccactagg gacgacgccg accgccgcct gccgggcctg
     1981 gtcgtagctc gtgcggcgcc aggattgcgg cacgccgtgc ctgaattgcc cgaacggcat
     2041 ccggccgcac gcccagacgg ccacgacaag aatgcagagg gcgtacgcgg gtccgatcgc
     2101 ccaggtcggc gctccgagct gcttgcgatg gccactggtc gacgtccggg cctgaagtgc
     2161 gttgcgcagt cgcgcgacgg tatcgaccgc tgcgagagtc aggatcggca cgaacggcgc
     2221 agagtattga tgcgtcaggg tccaatggtt cgggttgttc gacagcaacc gctcggcgag
     2281 cagcgggacg gcgagcagca cgaagggcga gccgagcggc atgaaaccga gcgggacgaa
     2341 cagccactcg agcagccgca ccttcgcgtg gggtgtgacc atcaggtgca acgtcgagag
     2401 cgggtgccgg acgacgtgcc atgcggcctg gagtggacct gagccaagag cagcgtagta
     2461 atcccagtaa tagccggact ttccgccgaa cgcagggata agcacccgtt cggcgagcac
     2521 ggtcgtcgca actccgacaa ctgccaggac gctcgccaag cgccaccttt gggtacgcaa
     2581 tccgacgacg acgccgaatg cggcgactac gatcccgaag tcttccttca ccaacaacaa
     2641 gagcagtgcg gcgatgacgg cggtgcgcaa ccgatcggcg tccaaccgct cgatcgcaat
     2701 cgccagcagg ggcaccgcga acgcgatctc gtgaaagtcg gcggccatgg cggcttgcag
     2761 gccccacgcc agtgcgtagg cagcagcgat gaggtag
//
LOCUS       DASWVF010000721         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_376658, whole genome shotgun sequence.
ACCESSION   DASWVF010000721 DASWVF010000000
VERSION     DASWVF010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376658"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VGD55_12735"
     CDS             complement(<1..411)
                     /locus_tag="VGD55_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871259.1"
                     /translation="MKKSFALLTAVVALGLTACSSASPSASRAPLAKATVDPTTGISS
                     VSFSNFNAAMAKITDIHPALEQLSADTAALAAVEDTAFSAADDQDVPSFTAACAQLKP
                     LGATWTADLANIKATFPGVLSPAELDPSEAELTLA"
     gene            698..2305
                     /locus_tag="VGD55_12740"
     CDS             698..2305
                     /locus_tag="VGD55_12740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0871260.1"
                     /translation="MSYAARGISYLVIAVTVVTAVLLAAFTVQRREALTAGTNSDSAV
                     LALTAMLDQETGVRGFLYTGDPVFLEPYLAGQADYAMQRLLVVKAARGDATSTRLAAL
                     EDSSANTWQQWATAVVAGRSASAIPPGNAVQEEVFGKQQMDDFRASNTALRTSLDRRR
                     DALLRKTGFVSTGIVIGLGAIFVLLGYLALRRASRRTIAQGAAEVAYRSRQAGFSDLI
                     QAVDTEDEAHDLLQRHLHRSIPGASITVLSRNNSDNRLAAVSALEPDSELAERLSSAE
                     PRSCLAIRLGRHHEDGTARDELISCKICSHVDGIATCQPLLVSGKVIGTVLIGHKRAL
                     TDAELRGVTDTVAQAAPVLANLKTIAIAENRASTDALTGLPNRRAMNDTLKRMVAHSG
                     RTAQPLAAIAFDLDRFKAINDRYGHEAGDAALSTVGECLREHLRDSDFAARIGGEEFL
                     ILAPDTDVEGARVLAEKLREALAREEVPQLLEAFTASFGIAVMPYHATSTEALLRRAD
                     RACYLAKERGRNRVEVATLEDAPARTV"
     gene            2637..>2876
                     /locus_tag="VGD55_12745"
     CDS             2637..>2876
                     /locus_tag="VGD55_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871261.1"
                     /translation="MARIAERKRVDGSSSWRVEWKLGGGRDGARQSLTMATEKDAVKL
                     SVLIAKHRNQLSATEVLDLITPTRITEPRGAGETVT"
BASE COUNT          548 a          925 c          914 g          489 t
ORIGIN      
        1 ggcgagagtc aactcggcct ccgaagggtc aagctcggcg ggtgagagaa ccccagggaa
       61 cgtcgccttg atgtttgcaa ggtcggcggt ccaggtggca ccaagaggct tgagctgcgc
      121 gcaggcagcc gtgaacgagg gaacgtcctg gtcgtcggcg gcgctgaacg cggtgtcctc
      181 gacggcagcg agcgccgcag tgtcagcaga aagctgctcc agcgccgggt gtatgtcggt
      241 gatcttcgcc atggccgcat tgaagttgct gaaggacacc gagctgatcc ccgtggtcgg
      301 gtccaccgtc gccttggcca gaggtgccct ggacgccgac ggggacgccg acgagcaagc
      361 agtcagcccc aacgccacaa cggcggtcag cagcgcgaac gatttcttca tgtgtctcct
      421 tgtgcgggac gcacggaaaa gaacccgtgg tgcctataca tcggcttttg gtcgagcacg
      481 aatgaaggct ttgttgtgtg ctcttgacct caatgctccc ggccccgcct atttcgcggt
      541 accgccgaat cgcctcgcgt caccggccct tttggcggtc cgtttgcgcg cggctagtcc
      601 gatcggctag atccacgcgc ggtcggcgga atccaaccga gcggatatcg ggacaaagtt
      661 ctcctcatga tcgtcctcgc acatccgatg gagacgggtg tcgtatgccg cgcgcgggat
      721 ctcctaccta gtgatcgccg tgaccgtcgt gaccgccgtc ctgctcgcgg cgttcacagt
      781 ccaacgccgc gaggcgctga ccgcgggcac gaacagcgac agcgccgttt tggcgttgac
      841 cgccatgctc gaccaggaaa ccggggtacg tggattcctc tacacaggtg acccggtctt
      901 cctcgagccc tatcttgcgg gccaagccga ctatgcgatg caacgactgc tcgtggtgaa
      961 ggccgcacgc ggcgatgcga cgtcgacgcg ccttgcggca ctagaagact cctcagccaa
     1021 cacttggcag cagtgggcga ccgcagtagt cgccgggcga agcgccagcg ccattccgcc
     1081 tggcaacgca gtgcaggagg aagtcttcgg caagcaacag atggacgact tccgggcgtc
     1141 gaacactgcg ctgcgcacca gtcttgaccg acggcgcgac gcccttctcc gcaaaaccgg
     1201 attcgtctcg accggcatcg tcatcggtct gggagccatc ttcgtgctcc tcggctacct
     1261 ggcgctgcgc cgcgcttcgc gccgcaccat cgcgcaggga gcagcggagg ttgcgtacag
     1321 gtcgaggcag gccggattct cggatctcat ccaagccgtc gataccgagg atgaggcgca
     1381 cgacctcctt cagcgacatc tgcaccgatc gatacctgga gcaagcatca cggtgctctc
     1441 gcgcaacaac tcggacaatc gccttgcggc cgtctccgcg ctcgagcccg attccgagtt
     1501 ggctgagcgt ctttcgtcag ccgagccgcg gtcatgccta gccattcgcc tcggccgcca
     1561 ccacgaggac ggcacggctc gtgacgagct catctcatgc aagatctgta gccacgtcga
     1621 cggcattgcg acgtgccagc cattgcttgt cagcggcaag gtgatcggca cggtcctgat
     1681 tgggcacaag cgcgcgctga cggacgccga actacgcggc gtcacggata ccgtcgctca
     1741 ggcggctccg gtgctggcga acctcaagac gatcgccatc gccgagaacc gcgcgtcgac
     1801 agacgcactc acggggctgc cgaaccggcg cgccatgaac gacacgctca aaaggatggt
     1861 cgctcattcc gggcgcaccg cccaaccttt ggcggctatt gcattcgacc ttgatcggtt
     1921 caaggctatc aatgatcgct acggtcacga ggctggtgat gccgctctct caaccgtcgg
     1981 cgaatgtctt cgcgagcacc tgcgtgacag cgactttgcg gcgcgcatcg ggggcgaaga
     2041 attcctgatc ctggcccccg acaccgacgt tgaaggtgcg cgtgtgctgg ccgagaagct
     2101 gcgcgaggcg ttggcccggg aggaagtgcc acaacttctc gaggcattca ccgcaagctt
     2161 cggcatcgcg gtcatgccct accacgcgac cagcaccgaa gcgctccttc gtcgggccga
     2221 tcgcgcgtgt tacctcgcga aagaacgcgg tcgcaatcgg gtcgaagtcg ccacccttga
     2281 ggacgcgccc gcacggacgg tctagccgaa ccggcgtgac ggccgacttc cgaaactgcg
     2341 aaaacctcga acccgtaggt tcgaggccgc cgacatcgtg cgaactcacg ccgtcgggat
     2401 gacaggattt gaacgagtca agggtcacgc tcaaaatgca tcgttgcagg tcacagcgct
     2461 gcgttgctgt gcactgggga cgcctggaag ggctatggtg agcactgggg acgcctaggc
     2521 gtccccgaaa acccttacgg cacaggatga caggactcac caatggccgc cgcgacgagt
     2581 ctggcgggct ggcgagtcca atcatcccga ccacgacgac aggacgtagg cacccgatgg
     2641 ccagaatcgc tgagcggaag cgcgtcgatg ggtcgagcag ctggcgcgtt gagtggaagc
     2701 tcggtggtgg ccgcgatggt gccaggcagt cgctgacgat ggcgaccgag aaggacgccg
     2761 taaagctgtc tgtcctgatc gccaagcatc gcaaccagct gagcgccaca gaagttctcg
     2821 acctgatcac gccaacccga atcactgagc ccagaggcgc tggcgagaca gtgaca
//
LOCUS       DASWVF010000722         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_351316, whole genome shotgun sequence.
ACCESSION   DASWVF010000722 DASWVF010000000
VERSION     DASWVF010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351316"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VGD55_12750"
     CDS             complement(<1..1387)
                     /locus_tag="VGD55_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0871262.1"
                     /translation="MGADDHFGQPFDVRDVLARRGKDRYELHRRYLNPQLPRMLHAIG
                     FDKVYARAEGAYLYDTDGVEYLDMLAGFGVFALGRHHPVVRQAIHDVVDAGLADLTQF
                     DAPPLAGPLAEALLEKVPHLDRVYFGNSGTEAVEAALKFARYATGRRRIIYCDHGFHG
                     LTAGSLSVNGSNAFRKGFGPMLPDTAIPFGDLRALEKELRRGDVAGFLIEPIQGKGVA
                     VSPPGFLPAAQDLLRAHKALLIADEVQCGVGRTGDFFAFQNDGVEPDLVATAKALSGG
                     YIPIGATIGRDWIFNKVYSSMDRVLVHDSTFGSNAQAMAAGLATLHVMEQENVVDNAR
                     KIGDLLQSRLAELVDRYELMSEVRGRGLMIGIEFGRPTSWKLRGSWMALQAARKGLFA
                     QTVVHALFHQHHILTQVSGDHLEVIKLIPPLIIGEREVDLFVDAFVDVMDEAHRGSGL
                     VWDFGKTLVKQT"
     gene            1579..2769
                     /locus_tag="VGD55_12755"
     CDS             1579..2769
                     /locus_tag="VGD55_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0871263.1"
                     /translation="MAGDDMDELGTALDTATGVRAVLDPVNERTIERPNDAIEPPAVL
                     GRARALVVPALRAAVDRLDGEYMRKIAAYHLGWLDADGQPISGDGVGGKAIRPTLAVL
                     SAEAGGGNASDGVAAAVAVELVHNFSLLHDDIMDRDVERRHRPTAWVVFGEGQAILAG
                     NAMLTAAIDVLVRDGVAGQRSLACLLSAVESLISGQSADLALGSLGSGGTLGTGGTGD
                     EIALVTTADVLAMEAGKTAALLACSASIGAIAVDAPAPVVDGLAGYGHELGMAFQLVD
                     DILGITGDAAATGKSSSSDVRAGKRSAPIVAALNSGTAASKMLARLLADGPPTSDDDV
                     TYATKLIDEAGGLEWAAHEADRRLARALAHLENLAHLENTPAPAGTTSDLAALARYVV
                     ERDR"
     gene            2789..4843
                     /gene="shc"
                     /locus_tag="VGD55_12760"
                     /pseudo
     CDS             2789..4843
                     /gene="shc"
                     /locus_tag="VGD55_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
BASE COUNT          793 a         1676 c         1746 g          791 t
ORIGIN      
        1 gggtctgctt caccagcgtc ttgccgaagt cccacaccag gcccgacccg cgatgcgcct
       61 cgtccatcac gtcgacgaac gcgtcgacga acagatcgac ctcgcgctca ccgatgatca
      121 gcggcgggat cagcttgatg acctcgaggt ggtcgccgga cacctgcgtc aagatgtggt
      181 gctggtggaa gagggcatgc acgacggtct gggcgaacag gcccttgcgg gcagcctgta
      241 atgccatcca cgagccgcgg agcttccatg aggtcgggcg gccgaattcg atgccgatca
      301 tgagtccgcg gccacggacc tcggacatca gttcgtagcg gtcgaccaac tcagcgagcc
      361 gtgactgaag caggtcgccg attttgcgcg cgttgtcgac gacgttctcc tgctccatga
      421 cgtgcagcgt cgcaagcccg gccgccatcg cctgagcgtt ggagccgaac gtcgagtcgt
      481 gcacgagcac gcgatccatc gacgagtaga ccttgttgaa gatccagtcg cgcccgatcg
      541 tcgccccgat cgggatgtag ccacccgaca aggccttggc cgtcgcgacg aggtcgggct
      601 cgacgccgtc gttttgaaac gcgaagaaat cgccggtgcg accgactccg cactgcacct
      661 cgtcggcgat cagcagcgct ttgtgggcac gtagcaagtc ctgcgcggcg ggcaggaacc
      721 ccggcgggga gacggcgacg cccttgccct ggatcggctc gatgaggaac ccggcgacgt
      781 cgccgcgccg caactccttc tcgagggctc gcaggtctcc gaacgggatc gccgtgtcgg
      841 gcagcatcgg cccgaagccc ttccggaacg cgttggaacc gttcaccgac agtgagcctg
      901 cggtcaggcc gtggaacccg tggtcgcagt aaatgatgcg ccggcggccg gtcgcgtagc
      961 gggcgaactt cagcgcagcc tcgaccgctt cggtcccgct gttaccgaag tagacccggt
     1021 cgaggtgcgg gaccttttcg agcagcgcct ctgccagcgg accggcaagc ggcggggcgt
     1081 cgaactgcgt cagatcggcc agacctgcgt cgacgacgtc gtgaatcgcc tggcgcacga
     1141 ccgggtggtg acgcccgagc gcgaagacgc cgaaaccggc gagcatgtcg aggtactcca
     1201 cgccgtcggt gtcgtagagg tacgcaccct cggcgcgcgc gtagaccttg tcgaagccga
     1261 tggcgtgcag catgcggggg agctgcgggt tgaggtagcg ccggtgcaac tcgtaacgat
     1321 ctttgccgcg acgagccagc acatcgcgga cgtcgaacgg ttggccgaag tgatcatcgg
     1381 cacccatcgg tgacggcacc tcctcgcacg gcagcctgtc gaccgcgctg cgcccgagga
     1441 tgccacgtca gggccatgtt gcgcacgcta acggaccgcc caccggccag aacggatcgg
     1501 ccaccgaggt gtctcatctg atatacagcg agtggacata actgtttact tagcacgtac
     1561 ttacccgttc tagaccacat ggcgggcgac gacatggacg agctgggcac cgccttggac
     1621 actgccacag gcgtgcgagc cgtccttgac cctgtcaacg agcgcaccat cgagcgtccc
     1681 aatgacgcca tcgagccgcc tgccgttctg ggccgcgctc gagccctcgt cgtccccgcc
     1741 cttcgggctg ccgtcgaccg gctcgacggc gaatacatgc gcaagatcgc ggcctaccac
     1801 ctcggctggc tcgacgcaga cggtcagccg atctcgggtg acggcgtcgg aggcaaagcg
     1861 atcaggccga ccctcgccgt gctgtcagcc gaagccggcg gcgggaacgc cagtgacggc
     1921 gtcgcggcgg cggtcgcggt cgagctcgtc cacaacttct cgctgctgca cgacgacatc
     1981 atggatcgcg acgtcgagcg ccggcaccgg ccgaccgcct gggtcgtgtt cggtgagggg
     2041 caggcgatcc tcgcaggcaa cgcgatgctt accgcggcga tcgacgttct ggtccgcgat
     2101 ggcgtcgccg ggcaacgcag cctcgcgtgc ctgctgtcgg cggtcgagtc gctgatcagc
     2161 gggcagtcgg ccgatctcgc actcggcagc ctcggcagtg gcggcactct cggcactggc
     2221 ggcaccggcg atgagatcgc actggtcacg actgctgacg tgctcgcgat ggaggccggc
     2281 aagaccgccg cgttgctggc atgttccgcg tcgatcggcg cgatcgcggt cgatgcgccg
     2341 gcgccggtag tcgatggcct ggccgggtac gggcacgaac tgggcatggc gttccagctc
     2401 gtcgacgaca tcctcggcat caccggtgat gccgccgcga ccggcaagtc gtcatcgtcc
     2461 gacgtccgcg ccggaaagcg cagcgcgcct atcgtggccg cgctgaactc gggtaccgcg
     2521 gcatcgaaga tgctggcaag gctgctcgca gatggtccgc ccacgagtga cgacgacgtc
     2581 acctatgcga cgaagttgat cgatgaagcc ggtggtctcg agtgggccgc ccacgaggcc
     2641 gaccgcaggc tggcgcgggc gctggcgcat ctcgagaacc ttgcgcacct cgaaaacaca
     2701 ccggcgccgg cgggaacaac ttccgacctc gcggcgctgg cacgctacgt cgtcgaacgt
     2761 gatcgctgat cgggcaggag gctacgtcgt gaccgcgcag acgaacaccg acctctccga
     2821 tggggtcagc gtcgccgttg agcgcgcgca ggcccacctc gtcggccggc aggacgctgc
     2881 cggctggtgg aagggcgagc ttcgaaccaa cgtcaccatg gacgccgaag acctgctgct
     2941 gcggcaattc ctggggatcc tgaaggtcga cgaaacgcag gaagccgcgc gctggatacg
     3001 ttcccagcag tgaccagacg gcacctgggc gacgttcgag ggcggccccg gcgatgtatc
     3061 gaccacgatc gaggcttatg ccgcgctgcg cctcgccggt gacggcgtcg acgagccgca
     3121 catggtcagg gcgcgagaat tcgtgatcgc cggcggcggc gtcgaagcga gccgcgtgtt
     3181 cacccgtatc tggctggcgc tgttcggcga atggtcgtgg gacgacctgc cctcgatgcc
     3241 gcccgagctg gtgttgctgc cgaggtggtt cccgctcaac gtctacgact ggggctgctg
     3301 ggcgcggcag accgtcgtcg ccatcacggt cgtcgcgacg ctgcgcccgg tgcgcccgct
     3361 gcccttctcg ctgagcgagc tgcgcagcgg cgtgcggccc gcaccgggca agaccggtgc
     3421 cgttgccacc gcgttcaccg ttctcgacag ggccctgaag ctctacgacc ggtcgccgat
     3481 caagccgggc cgctcgacgg cgttcaagca cgccaccagg tgggtgctcg atcggcaaga
     3541 agccgatggc gggtggggcg gcatccagcc gccgtgggtc tactcgatcc tcgcgctgca
     3601 cctgactggc cggccactcg acgacccggc gctggtcgcc gcgatcaagg gcctcgacgg
     3661 cttcctgatt cgtgaggaga ccgacgaagg cgtcgtccgc cgcctcgaag cttgccaatc
     3721 accggtctgg gacacctgcc tggccgtcat cgccttgctc gattcggcga tgcccgccga
     3781 cgacccgacc gtgcagaagg cggtcgactg gctgctcggt gaagaggtca gggtcaaggg
     3841 cgactgggcg gtgcgccggc ccgacctggc ggcggccggc tgggcgttcg agttcgccaa
     3901 cgacgcgtac cccgacatcg atgacaccgc tgaggtcgta ctagcgctgc gccgcatcgg
     3961 cctcgagtcg cggcccgacg tctcggcagc ggtcgaccgc ggcatcaagt gggcggtcgg
     4021 gatgcagtcg cgcgacggcg gctggggcgc gttcgacgcc gacaacaccc ggcggctggt
     4081 tgagaaggtg ccgttctgcg acttcggtgc ggtcatcgat ccgccgtcgg ctgacgtgac
     4141 ggctcacgtc gtcgagatgc tcgcgcacag cgggatgacg cagaccgagg cgtgtcggcg
     4201 cggcgtgcgg tggctgctcg acgcgcagga ggccgatggt tcgtggttcg gtcggtgggg
     4261 cgcgaattac gtctatggca ccggcgctgt cgtgccggct ctggttgccg caggggtggc
     4321 caccgactcg ataccggtgc gccgcgccgt ccgctggatc gagaagcatc agaaccccga
     4381 cggtgggtgg ggcgaggacc ttcggtcata cgtcgacgac gagtggcgcg gtcgcggtga
     4441 gtcgactccg tcgcagacgg cgtgggcgct gctcggcctg cttgcggtcg acgccgatac
     4501 cgatgctgtc gcgcgcggcg tcgactggtt ggtgcgcacg cagcgtcgcg acggcggctg
     4561 ggacgaagac ctgtacaccg gcaccggatt cccgggcgac ttctacatca actacgagat
     4621 gtaccgggtc gtgtttccgc tgagcgcgct cgggcgttac ctcgcggccg ggaaaggtgt
     4681 catcgacctc actgacggca cggtcgatgt cacggacgcc acggtcgatc tcacggacgc
     4741 cacggtcgat ctcaccgaca gcgtctcgtc ggtcgacctg acgaccctgc gcgagcaacg
     4801 cgccggaacc gacgagcctg ccgggaaagc cggcgcctcg tgaacgatct gctcgtcgtc
     4861 acggcgctgc gcgcggagta cgcggcgctc gccggccggg tgcccggcgc aacgctcgcc
     4921 cggtgcggca tgggccctgc gcgggtcgac gcctggctcc cgcgcatggc ggaacttgcc
     4981 cctgacgccg ttgtcgtcgc aggcgt
//
LOCUS       DASWVF010000723         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_122027, whole genome shotgun sequence.
ACCESSION   DASWVF010000723 DASWVF010000000
VERSION     DASWVF010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122027"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="dnaA"
                     /locus_tag="VGD55_12765"
     CDS             <1..1405
                     /gene="dnaA"
                     /locus_tag="VGD55_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0871264.1"
                     /translation="EDTVVLAAPHEFAKDVLESRLRPALTSALAAEIGREVRVAVTVE
                     SSAGSEAGDDLGNLDEQRSDDGPRTLAGSEAGSDTGPDGGSENARRSETDRGNQQGSQ
                     QSQPTRSHLGTGREPGGRREIEPARLNPKYTFETFVIGASNRFAHAAAVAVAEAPAKA
                     YNPLFIYGESGLGKTHLLHAIGHYAMGLFHGARVRYVSSEEFTNDFINSIRDGKAEGF
                     RRRYREVDVLLVDDIQFLENKEQTQEEFFHTFNTLHNANKQIVISSDRPPKQLVTLED
                     RLRNRFEWGLLTDVQPPELETRIAILRKKAAQERLNVPNDVMEYIASKIASNIRELEG
                     ALIRVTAFASLNRQGVDLSLAEVVLKDLIPDSQGPEITAATIMGATASYFGISLEDLC
                     GSSRSRVLVTARQVAMYLCRELTDLSLPKIGQLFGGRDHTTVMHADRKIRSLMAERRS
                     IYNQVTELTNRIKSQAK"
     gene            1740..2912
                     /gene="dnaN"
                     /locus_tag="VGD55_12770"
     CDS             1740..2912
                     /gene="dnaN"
                     /locus_tag="VGD55_12770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0871265.1"
                     /translation="MKFRVDRDVFAEAVAWAARSLPVRPPVPVLAGLLLEVSESPAAL
                     ALSSFDYEVSANAQIEISAAEPGRALVSGRLLAEISRSLPALPVDVSVEGGRVVVLCG
                     STRFTMPTLPVDDYPALPEMPATAGSIGADVFAAAVSQVAIAAGRDDTLPVLTGVRIE
                     IEDDRITLAATDRYRLAVRELHWQPERSGISVVALVPARTLADTAKALVGGAQVTVSL
                     STGGAGEGLIGFSGPAGSGQIGQRRTTTRLLDGEYVKYRSLLPDDSSANAFVETAGLV
                     EAVKRVSLVAPRSTPVRLTFNDDQVTLEAGTSDDAQALESLDAKFVSRDGSTEEFSIA
                     FNPAYLLDGLNAVESETTRIDFTSPTKPAVLRGSKEADTSATADYRYLLMPVRLSG"
     gene            2982..>3403
                     /locus_tag="VGD55_12775"
     CDS             2982..>3403
                     /locus_tag="VGD55_12775"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0871266.1"
                     /translation="MTAADFEIGLIGLGKMGGFMRERMRKGGLTVVGYDRNPQLSDAS
                     SLEDLVSKLSAPRKVWVMVPAGEPTRQTIDELSELLEAGDLVVDGGNSKYTDDQVHAA
                     KLGTQGIGFVDAGVSGGVWGLKNGYALMVGGDAAHVSLV"
BASE COUNT          663 a         1085 c         1068 g          587 t
ORIGIN      
        1 cgaggacacc gtcgtcctcg cggcccctca cgagttcgcc aaagacgtgc tcgaaagcag
       61 gctgcgtccc gccctgacgt ccgcccttgc agccgaaatc ggtcgtgagg tccgggttgc
      121 agtgacggtc gagagttccg ccgggtccga ggctggagac gacctcggaa acctcgacga
      181 gcaaagatcc gacgacggtc cacgcaccct tgcgggctct gaggccggct ctgatacagg
      241 ccctgatggt ggctctgaga acgcgcgtcg ttcggagact gaccgcggca accagcaagg
      301 cagccaacaa tcccagccga cacggtcgca cctgggcacc gggcgcgaac ctggcggcag
      361 acgcgagatc gagccggccc gcctcaaccc caagtacacc ttcgaaacat tcgtcatcgg
      421 ggcgagcaac cgtttcgcgc acgcggcagc ggtcgcagtc gccgaagccc cggcgaaggc
      481 gtacaacccg ttgttcatct acggcgagtc aggcttgggc aaaacccatc tcctgcacgc
      541 aattggccac tatgcgatgg ggctctttca cggcgcccga gtgaggtacg tcagttcaga
      601 agagttcacg aacgacttca tcaactcgat tcgcgacggc aaggccgaag ggttccgccg
      661 ccgttatcgc gaggtcgacg tgctgcttgt cgacgacatc cagttcttgg agaacaagga
      721 gcaaacgcag gaggagttct tccatacttt caacacgttg cacaacgcca acaagcaaat
      781 cgtgatctca agtgaccggc cgccgaagca actggtcacc cttgaggatc ggctccggaa
      841 ccgcttcgag tggggcctgc tgaccgacgt ccaaccgccc gaacttgaaa cccgcatcgc
      901 catcttgcgc aagaaggccg cccaagagcg attgaacgtt cccaacgacg tgatggagta
      961 catcgccagc aagatcgcga gcaacatccg cgaactcgag ggtgctctga tccgggtgac
     1021 cgcgttcgcc agccttaatc gccaaggcgt tgacctgtcc ctcgccgagg tcgttctgaa
     1081 ggacctcatc cccgacagcc agggcccaga aataaccgcc gcgaccatca tgggagcgac
     1141 ggcgtcctac ttcgggatct cactcgaaga tctgtgcggg tcctcccgca gtcgggtgct
     1201 cgtcacggcg cgacaagtgg cgatgtacct gtgccgcgag ttgaccgacc tctccctacc
     1261 caagatcggc cagttgttcg gcggccgcga ccatacgacg gtcatgcacg ccgaccggaa
     1321 gatccgttcc ctcatggccg agcgccgttc gatctacaac caggtcaccg agctcacgaa
     1381 ccgcatcaag tcgcaggcga aatagcatga ttttccgccg ctgtgcacag cctggggaca
     1441 accttgtgga ccagcggtgg gtttcggtgg gtggagcgac tttgccgtgg gacgacctgt
     1501 ggggagccgc tcagatccaa ccgcttgtcc ccagcgaact caccggtcgg gccacgtcat
     1561 ccacaagtag ccaggcccgc ttcatgccga ctgacctgcg gaaagggcag ttttccacag
     1621 tgtccacccc accgatgacg acttccttga gacaaggatt aagaatgaca caagcagtaa
     1681 ggacggcagg gggttccaat gatgggtagc gcaagccggg cgggcagggt gggtatcgca
     1741 tgaagttcag agtcgaccgt gacgtcttcg ccgaggcggt ggcgtgggct gctcgtagcc
     1801 tcccggtgcg cccacccgtc ccggtgttgg cagggctcct cctcgaggtg tcggagtctc
     1861 cagccgcatt agcactttcc agcttcgact acgaggtctc ggcaaatgcc cagatcgaga
     1921 tcagcgctgc cgagccgggg cgcgctctgg tgtcggggcg gctcctcgcc gagatcagcc
     1981 gcagcttgcc tgcgctgcca gtcgacgtct cggtcgaggg cggccgggtc gtggtcctgt
     2041 gtggcagcac tcgtttcacc atgccaacct tgccggtcga tgactatccg gcgttgcccg
     2101 aaatgccagc aaccgcaggg agtattggcg ccgatgtctt tgctgcggcg gtctcgcagg
     2161 ttgccattgc agctggccgc gatgacacct tgccggtgct gaccggcgtc cggatagaaa
     2221 tcgaagacga tcggatcacg ctggcggcga ccgaccgcta ccgcctggcg gtgcgcgaac
     2281 tgcattggca gcccgagcgc agcggcattt ccgtggtcgc cttggtgcct gcccgcacct
     2341 tggctgatac ggccaaagcg ctggtcgggg gagcccaggt gaccgtgtcg ttgtcgaccg
     2401 ggggcgccgg cgaaggcctg atcggtttct ccggccccgc tgggtcgggg cagatcggtc
     2461 agcgccgcac cacgacccgg ttactcgacg gcgagtacgt aaaataccga tcactgttac
     2521 ctgacgactc gtccgcgaac gcgtttgtcg agactgcggg cctggtcgag gcggtgaagc
     2581 gagtgtcgct cgtcgcgcca cgcagcacac cggtgcggct gacgttcaac gacgatcagg
     2641 tgaccctcga ggctggcacg agtgacgacg cgcaggcgct ggagtcactc gacgcgaagt
     2701 tcgtcagccg tgatggctcg accgaggagt tctcgatcgc gttcaacccc gcctaccttc
     2761 tcgacggtct taacgcagtc gagtccgaga ccacacggat cgacttcacg tcgccgacaa
     2821 aaccagccgt gctacgcggc agcaaagaag ccgacacgtc agcaaccgcc gactatcgct
     2881 atctcttgat gccggtacgg ctctccggct gagccgactg catatgcggg ctcgatgtgg
     2941 gctcgactcg acaacacaaa catgagaacg cggaggaacg catgacagca gccgacttcg
     3001 agatcgggct gatcggtctc ggcaaaatgg gcgggttcat gcgcgagcgc atgcgcaaag
     3061 gcggcctgac tgtcgttggg tacgaccgca atcctcagct cagcgacgcg agctcactcg
     3121 aggatctcgt gtcaaagctc agtgcgccgc gcaaagtctg ggtgatggtg cccgccggcg
     3181 aaccgacgcg gcaaaccatc gatgagctaa gcgaactgct cgaggccggc gacctcgtgg
     3241 tcgacggcgg caactcgaaa tacaccgacg accaagtcca cgccgcaaag ctcgggacgc
     3301 agggcatcgg attcgtcgac gccggtgtgt ccggtggcgt gtggggtctg aagaacggct
     3361 acgcattgat ggtgggcgga gacgccgccc acgtttccct tgt
//
LOCUS       DASWVF010000724         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_198592, whole genome shotgun sequence.
ACCESSION   DASWVF010000724 DASWVF010000000
VERSION     DASWVF010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198592"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..2137
                     /locus_tag="VGD55_12780"
     CDS             272..2137
                     /locus_tag="VGD55_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0871267.1"
                     /translation="MTMRRWLLIAMSLGMLGACGENDPQASNAAPAFAAPAAVSDWAQ
                     GRDDDHEGDRRGDHDDDRGRRIRHVLLISVDGMHQSDLHWYVANHPHSTFAKLIHTGS
                     EYTNAAASNPSDSDPGGTALMTGGNPKSTGVFYDVEYSHKVDEAGAACTPGQPATGGD
                     VIYDSPDDALASVPDFIDPTSGTFPSFDEDGSIFPNGVDTNPAAIMDLKFDPETSLNP
                     GTFPVDPKTCKPITPWDYLGDNTIFQVIHHAGLRTAWSDKHEVYASFNGPGSNGQSID
                     DLFSPEIDSQAVMPDGSPYPQDDDWAHINAATKQYDGYKVQAILNEIDGLDHSGKTHV
                     GTPAIFGMNFQTVSVAEKIRSTPTTLIGPDANGHYTTSDPEAGGYQWVSGQLVPGPVL
                     SSALDYVDAQLGRIVSAIHKDGLAGSTTIIVTAKHGQSPQDPNKLITVQDGPIISAIN
                     AAWAQAHPGNTSLIVAGTDDDLWQSYLSDNSQAACDFVKSYLWNHTANGFDVNKNPVT
                     VQHSGLAEIWAGAAAAHFFGVSVDNGKYPDVFGKVQVGIVYAKPTKLAEHGGMNTGDR
                     HVLMVVSGPGIPVQVESTSVETTQVAPTVLALLGLNPHALTAVEIEGTRVLPGLR"
BASE COUNT          485 a          879 c          772 g          428 t
ORIGIN      
        1 agcgcgcgcg cgggtctggc cgccgcccgc tctccccgac aatcgcaacg ctagtcagat
       61 agccccccga aacgcatccg cgtttcgttc aaccgaggtc ggggccgctc atgctgtatc
      121 gagactcgac cggatgagct ccccgtcggc ggtgcactcc gtgcctctcg tacatgcggg
      181 tgcgtagtgc aacggccgaa aggtttgcaa ggtcgacaca atctgccagc cggaaccgcg
      241 atacacgggc tgtcccacaa aggagcggac tatgaccatg cgacgctggc tgctgattgc
      301 gatgtctcta ggaatgctgg gtgcgtgcgg agagaacgat ccgcaggctt cgaacgctgc
      361 gccggcgttt gcggcaccgg cagcagtgtc cgactgggcc cagggtcgtg acgacgacca
      421 tgagggcgac cgccgagggg atcacgacga tgaccgcggg cgacgcattc gccacgtcct
      481 tctgatcagc gtcgatggca tgcaccagag tgaccttcac tggtacgtcg ccaaccaccc
      541 acactctacg tttgccaagt tgattcacac gggcagcgag tacaccaatg ccgcggcctc
      601 caacccgtcg gactccgacc cgggtggcac cgcgctgatg accggcggta acccaaaatc
      661 gaccggcgtc ttctatgacg tcgagtacag ccacaaggtc gacgaggcgg gcgctgcctg
      721 caccccgggc cagccggcca ctggtggtga cgtcatctat gactcgccgg atgacgcgct
      781 ggcgagcgtc cccgacttca tcgaccccac gagtggcacc ttcccgtcgt tcgacgagga
      841 cggctcgatc tttccgaacg gcgtcgacac caacccggcc gcgatcatgg acctgaagtt
      901 cgacccggag acgtcgctca acccgggcac gttcccagta gacccgaaga cctgcaagcc
      961 gatcaccccg tgggactacc tcggcgacaa cacgatcttc caggtcatcc accacgccgg
     1021 cctgcgcacc gcttggtctg acaagcacga ggtctacgcg tcgttcaatg gtcccggctc
     1081 gaacggccag agcatcgacg acctcttctc gccggagatc gactcgcagg ctgtcatgcc
     1141 ggacggctcc ccttacccgc aggacgacga ctgggcgcac atcaacgccg ccaccaagca
     1201 gtacgacggc tacaaggtcc aggccatcct gaatgagatc gacggtctcg accactcggg
     1261 caagactcac gtcggcaccc cggcgatctt cggcatgaac ttccagaccg tctcggtcgc
     1321 tgagaaaatc cgctcgacgc cgacgacgtt gatcggtcct gacgcgaacg gccattacac
     1381 caccagcgat ccagaggccg gtggttacca gtgggtcagc ggtcagctcg ttcccggccc
     1441 cgtcctctcg agcgcgctcg actacgtcga cgctcaactt ggccgcatcg tttctgcgat
     1501 ccacaaggac ggcctcgcgg gctcgacgac gatcatcgtc acggcaaagc acggccagtc
     1561 gccgcaggat ccgaacaagc tcatcaccgt ccaggacggc ccgatcatca gcgcgatcaa
     1621 cgccgcctgg gcacaggcgc acccgggcaa cacgagcctg atcgttgccg gaaccgacga
     1681 cgacctgtgg cagtcctacc tgtcggacaa ctcgcaggct gcttgcgact tcgtcaagag
     1741 ctacctgtgg aaccacaccg ccaatgggtt cgacgtcaac aagaaccccg tcacggtcca
     1801 gcactcgggc ctcgccgaga tctgggccgg cgctgccgcg gcccacttct tcggcgtctc
     1861 ggtcgacaac ggcaagtacc cggacgtctt cggcaaggtc caggtgggca tcgtctacgc
     1921 gaagccgacc aagctcgccg agcacggcgg tatgaacacc ggcgaccgac acgtcttgat
     1981 ggttgtcagc ggcccgggca taccggttca ggtcgagtcc acctccgtcg agaccaccca
     2041 ggtcgcaccg acggtcctcg ccctcctggg gcttaaccca cacgcgctga cggccgtcga
     2101 gattgagggc acgcgggtcc ttccgggcct tcggtagacc gcaggctaga tggtgaccgt
     2161 gcgccggaag cacgccggtt aattggcagc actaaacagg aggccggggc gaatcgcccg
     2221 gcctcgtctg ctttcggcaa cctgaccgcc ggtctcgttc aatgagggcg aggccggtcg
     2281 cctgcggcgg taggtcctcg acgccagtgc tttgtcttct gaccggatcg ctgaccgaga
     2341 gctcgtggaa tgtccgcact ctgcgcctcg agcgcaccgg ctcgggcgga tcagccggtg
     2401 ttgattccga gcgtgggcaa caactacgtt cacgtgtggg ataactcgac ggaagacagc
     2461 agcttcggcg tgacgtctcc gatcgagttg acagccatcg atccgcacag cggtcatgta
     2521 ttcagggcgc tgtcggttcc tcccgatcag gtggtcacga gctt
//
LOCUS       DASWVF010000725         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_45398, whole genome shotgun sequence.
ACCESSION   DASWVF010000725 DASWVF010000000
VERSION     DASWVF010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45398"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGD55_12785"
     CDS             <1..693
                     /locus_tag="VGD55_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871268.1"
                     /translation="MPAEYTITTPRPATPATSSLRNFGVAFLASGLLGVAIGIVNLSY
                     PAAVPKDRWSYPFDATSQWVVSSVLSITHLLAIAGLVGVLIASPYGRSRAARIGIWAA
                     IIGCAGLAGCELLSGAVGTRNNNSSFAGAVNGAFGVASLLTAAGSIVAGVVIVRRVGR
                     RSGWSAILWSGVVLVVLVTPASIGGNLVFETVALTLWSLLYVPLGQALIRSRGVVLED
                     DGLQRRSSFLRT"
     gene            862..1698
                     /locus_tag="VGD55_12790"
     CDS             862..1698
                     /locus_tag="VGD55_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0871269.1"
                     /translation="MTSRATAQPAVTTSTAPTTAAASASAPGQGKSIVVIGHSGATGY
                     DSDAGTVEASGRQNAADGRENSWATGSNPAVDSIYQRLVAKSPEYAKHQFNLAADGAT
                     VTDMISQADYLPQVHPAPAVVFVQGGDNDMKCDGTDAQNYAPFQASFTTLLHDITTQA
                     PKASVYVLSMTISAASYANVIAGLPSVKAQNSGGSPCAPFDANGNRSASGIAYQQDDI
                     DHYDKAMAAACATIPVCHYDNGAMQRLGVVPADMTEDGNHLTVSGLRKYADLVWATFF
                     QN"
     gene            1712..>1971
                     /locus_tag="VGD55_12795"
     CDS             1712..>1971
                     /locus_tag="VGD55_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871270.1"
                     /translation="MRRVILAGASALVVLVVPTAFATDAGANPAALTCGQTVTHSIVL
                     THDLLNCQGDGLKVGASHITVDLNGHLIDGVNAAGSIGVNDNG"
BASE COUNT          366 a          661 c          596 g          348 t
ORIGIN      
        1 atgcccgccg aatacaccat cacgacaccc cgccccgcca cgccagccac cagctcgctg
       61 cgcaacttcg gtgtggcctt cctggcatcg ggactcctcg gggtagcgat tggcatcgtc
      121 aatctcagct atcccgccgc cgtgccgaag gaccgttgga gttatccctt cgatgcaacc
      181 tcccagtggg tcgtgagcag cgtgctctcg attacccacc tgctcgccat cgcgggactc
      241 gtaggcgtcc ttatcgcgtc cccttacggt cgttcacgcg cagccagaat cggcatctgg
      301 gcggcgatca tcggttgcgc gggcttagcg gggtgtgagc tgctcagcgg cgccgtagga
      361 acacgcaaca acaactcgtc gtttgcgggc gcagtgaacg gggcgttcgg cgtcgcatcg
      421 ttgctcaccg cagcaggctc gatcgttgcc ggcgtggtga tcgtgcgccg cgttggacgt
      481 cgaagcggtt ggtcggcgat cttgtggtcg ggagtcgtgt tggtcgtcct ggtcacaccg
      541 gccagcatcg gcggcaacct cgttttcgaa acggtcgccc tgacgctctg gagcctgttg
      601 tacgtaccgc tcgggcaagc gctaatccgc tcgcgcggcg tggtccttga ggacgacggg
      661 ttgcagcgca gatcatcctt cctgcggacg tagccgacgg cgtgtgcact ctaaattcgc
      721 caacagtcga tcattcgcca acagtcgatc ggactgctgg tccagccggt cggaggggag
      781 tctgcaatga ggagagcaag tgcgccgctt gcactgctgc tgtgtacagc aatcgggacg
      841 gcgagttgct cgtccagtca cgtgacctcg cgtgctacgg cgcaaccggc cgtcacaact
      901 tctaccgcac cgactacggc ggcagcgtca gcgtcggcgc cgggtcaggg gaagtcgatc
      961 gtcgtcatcg ggcactccgg cgccaccggc tacgactccg atgccggcac ggtcgaggcg
     1021 agcggcaggc aaaacgccgc tgatggccga gagaactcgt gggcgactgg tagcaacccg
     1081 gccgtcgaca gcatctacca acgcctcgtc gcgaagtcac cggaatatgc caagcaccaa
     1141 ttcaacctgg cggctgacgg cgccaccgtc accgacatga tctcccaggc ggactacctt
     1201 ccccaagtgc atccggcacc ggcggtcgtc ttcgttcaag gcggcgacaa cgacatgaaa
     1261 tgcgacggca ccgacgcgca gaactacgca ccgttccaag cgagcttcac caccttgctg
     1321 catgacatca ccacgcaagc gcctaaggcc agcgtctacg tactgagcat gacgatcagc
     1381 gctgctagtt acgccaacgt catcgcgggg ttgcccagcg tgaaagccca gaactccggt
     1441 gggtcgccgt gcgcaccctt cgacgctaac ggcaaccgat cggcgtcggg catcgcctac
     1501 cagcaagacg acatcgacca ctacgacaag gctatggccg cggcctgcgc gacgatcccc
     1561 gtgtgccact acgacaacgg cgccatgcag cgccttggcg tcgttcccgc cgacatgacc
     1621 gaggatggca accatctcac tgtcagcggt ttgcggaaat acgccgacct ggtctgggcc
     1681 accttcttcc aaaactgaaa ggaactccga tatgagacgc gtcattcttg ccggtgcttc
     1741 cgcgctcgtt gtgctggttg ttccgacggc gttcgccacc gacgctggcg ccaacccggc
     1801 cgcgctgacg tgcggccaga cggtgacgca cagcatcgtg ctcacccacg acttgctgaa
     1861 ctgccaaggc gacggcctga aggtcggtgc aagccacata accgtcgacc tcaacggcca
     1921 cctcatcgat ggcgtcaacg ctgccggcag catcggcgtg aacgacaacg g
//
LOCUS       DASWVF010000726         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_275106, whole genome shotgun sequence.
ACCESSION   DASWVF010000726 DASWVF010000000
VERSION     DASWVF010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275106"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGD55_12800"
     CDS             complement(<1..213)
                     /locus_tag="VGD55_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEY0871271.1"
                     /translation="MLSEGYLDGLASLPLSEVRELRDQAEQEEADLSYLRRMLQGRVD
                     IINAELPRRRGDAGGSIIDQLPRILAD"
     gene            545..>1618
                     /locus_tag="VGD55_12805"
     CDS             545..>1618
                     /locus_tag="VGD55_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0871272.1"
                     /translation="MLKGELTTTPIEVALSSLANEQATGCLHVVDDAGSEALIYLKAG
                     LVYAVSVPGQRPQLGARLISSNALVPENLAEALEAQRTELQGWRLGELLVHLGFVEQD
                     VVEAFVQEQVRDGLTDLLAWTSGRWRFRKGEKTREDVAPPTPFDQLLDQVSIRKSTWE
                     EIVDAVHGPNAVPMLAAHGGEAHITLDSDTWSLLCKIDGERSIADLARECGFTLFEAG
                     TVVRVLVEAGLVDVEEDVGPDGAPVYDAADDTADDDEVEDDGPDAELTQAEEPVRDNF
                     SRTAALAAAFALSAREPVADPPAVADEQHEWNGPEPREAASMSSLAEVSEALTNALGP
                     RTDVGASFVDPFHVPDENRMRAAT"
BASE COUNT          280 a          531 c          530 g          277 t
ORIGIN      
        1 gtcggcgagg atgcgtggca actggtcgat gatgctgcct cccgcgtcgc cgcggcgccg
       61 gggcagctcg gcgttgatga tgtcgacacg gccctggagc atccggcgca gatacgacag
      121 atccgcctct tcttgctcgg cctggtcgcg cagttcgcgc acttctgaca gcggcaggct
      181 agccaatccg tcgaggtaac cctcggaaag cacccggtca atgcggcggt gaccgcttgg
      241 ctcatctctg cggtcgtcgg acacctgatg cctccgtgag tcgtggtgcg gcgttcgggt
      301 cagcttcctt tagcccaatt ggagtaacgc cgcgaagcca atcggactga cctcgcccat
      361 ggtgccatgt cggggagaag ctggcacgaa tcgatcatta tccgagacgc caaatacgcc
      421 tggatgttcg accggatggc tcaatgctcc cgccccagtt gccgatggca acgatgcagg
      481 gttaatcggc accttgcgtc catttgcgtc agcgcaacag ccgttccaag gtgggaggtc
      541 gcccgtgctc aaaggcgaac tcacgaccac ccctattgaa gttgcgctca gcagcctcgc
      601 aaacgagcag gccactggct gcctccatgt tgtcgacgac gccggcagcg aggctctcat
      661 ctacctcaag gctgggctgg tctacgcggt ttcggtaccg ggtcagcgcc ctcagctcgg
      721 cgctcggctg atctcgagca atgccctcgt ccccgagaac ttggcggagg cgctcgaggc
      781 gcagcgcact gagctccagg gctggcgact cggtgaactg ctcgtgcacc tcggcttcgt
      841 cgaacaagac gtggtcgagg ctttcgtgca ggagcaggtc cgcgacggct tgactgacct
      901 tcttgcctgg acgtcgggtc gctggcgttt ccggaagggc gaaaagactc gcgaggacgt
      961 cgcaccgccg acgccgttcg accagttgct cgatcaggtc agtatccgga agtcgacctg
     1021 ggaagaaatc gtcgacgcgg tgcacggacc gaacgcggtg ccaatgctgg ccgcccacgg
     1081 cggcgaggcc cacatcaccc tcgactccga cacgtggtcg ctgttgtgca agatcgacgg
     1141 ggagcgcagc atcgccgatc tcgcgcgcga gtgtgggttc acgttgttcg aggccggcac
     1201 cgtcgtgcgg gtactcgtcg aggccggtct ggtcgacgtc gaggaagacg ttggcccgga
     1261 cggcgcgccg gtgtacgacg ctgccgacga caccgctgac gacgatgagg tcgaggacga
     1321 cgggccagac gccgaactca cgcaggccga agagcctgtg cgtgacaact tctcccggac
     1381 cgcggcgctc gcggcagcct tcgctttgag cgcgagggag ccggtcgcag atccacccgc
     1441 cgtcgccgac gagcagcacg agtggaacgg gccggagccc cgcgaagccg ccagcatgtc
     1501 atcgctcgcc gaggtctccg aagccctcac caacgcgctc ggcccacgca cagacgttgg
     1561 ggcgtccttc gtagatccct tccacgtgcc cgacgagaac cgcatgcgcg cggcgact
//
LOCUS       DASWVF010000727         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_479151, whole genome shotgun sequence.
ACCESSION   DASWVF010000727 DASWVF010000000
VERSION     DASWVF010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479151"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="metF"
                     /locus_tag="VGD55_12810"
     CDS             <1..878
                     /gene="metF"
                     /locus_tag="VGD55_12810"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0871273.1"
                     /translation="TIKELLAQGGHSFSFEFFPPKTEQGERSLFQTIRELEQLRPTFV
                     SVTYGAGGSTRDTTVRMTERIATETTLTPVGHLTAVNHSVAELRRVIGQYADAGVRNV
                     LALRGDPPGDPQAEWVAHAAGFQYAEELVALLKNSGQFCVGVAAFTRKHPRSPTVDDD
                     IANFVRKCRAGADFAITQMFFSVDDYLVLRDRVAAAGCDVPIIPGIMPLTSIGQIEKF
                     SILSGDDFPPALASKLLAVADDPVAVRAMGLEVASEMCQRLLAEGVPGLHFITLNKST
                     ATREIYQNLGLPDLQ"
     gene            1048..1860
                     /locus_tag="VGD55_12815"
     CDS             1048..1860
                     /locus_tag="VGD55_12815"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0871274.1"
                     /translation="MSLNPAVRSVSVGVAAALIVVAAYAWGRGGSARAATNAATTIPA
                     QTLAAPASAGPTGSAAGAGISVGGTGRVTGTPNLLRLSTSVNVTKPTVTDAMQDANTV
                     MAAVQQKLKADGVAAADLQTSGLSVQPNYSYANNTPKLTGYQVSENLSVVLRDLNRAG
                     SIISDAAQAGGDALQIGGATLDLDQDDTLIAQARQAAFADAESKAKAYATAAGRTLGA
                     VTSISETTDSQPQQFDNYAFAAAAPAASGAPVPIQAGSQDVTVNVAVTWALN"
BASE COUNT          326 a          619 c          623 g          325 t
ORIGIN      
        1 gcactatcaa ggagctgctt gcgcagggcg gtcattcgtt ctcgttcgag ttcttcccgc
       61 cgaagaccga gcaaggcgag cgatcgttgt tccagacgat tcgcgaactc gagcagctgc
      121 gcccgacgtt cgtctcggtc acctacggtg ccggaggctc gacccgcgac acgacggtcc
      181 ggatgaccga gcgcatcgcc accgagacga cgttaacgcc agtcggacac ctcaccgcgg
      241 tgaaccactc cgtcgcagag cttcgacgag tcatcggtca atatgcggac gccggagtgc
      301 gcaacgtcct ggcattgcgt ggggatcctc cgggtgaccc gcaagccgaa tgggtcgcgc
      361 atgctgcggg attccaatac gcggaagaac tcgttgcgct gctgaagaat tcgggccagt
      421 tctgcgtggg tgtcgctgcg ttcacgcgca agcatccgcg gtcgccaacg gtcgacgacg
      481 acatcgcaaa cttcgtgcgc aagtgccgcg ctggcgccga tttcgcgatc actcagatgt
      541 ttttctcggt tgacgactac ctcgtgttgc gcgaccgggt cgctgccgcg ggctgcgacg
      601 tgccgatcat cccgggcatc atgccgctga cctcgatcgg gcagatcgag aagttctcga
      661 tcctgtccgg tgacgacttc ccaccggcgc tggcctcgaa gttgcttgcg gttgctgacg
      721 atcccgtcgc ggtgcgggcg atggggctcg aggtggcgag tgagatgtgt cagcgcttac
      781 tggccgaggg cgtgcccggg ctgcacttca tcacgttgaa caagtcgacc gcgacccgtg
      841 agatctacca gaacctcggc ctgcccgacc tgcagtagcc gtccgccgtc cgacgcccgc
      901 tgtccgaccc agatcgtccc ggatcgtgga gtcctggcgt tgctctagcg accgccagac
      961 tccacgatca ccgagcgggc ggcgcggaac cggcggggag ttggcggcgt ccaaccggca
     1021 cgtttgcacc cactgccgga ggccgtcatg tcgctcaacc ccgctgtgcg ttcggtttct
     1081 gtcggagtcg cggccgccct gatcgtggtg gccgcttatg cgtgggggcg tggtggctcg
     1141 gctcgggcag cgactaacgc tgcgacgacg attccggccc aaaccctcgc tgcgccagcc
     1201 agtgcggggc cgacgggttc ggccgcgggc gcaggcatca gcgtgggtgg gaccggacgc
     1261 gtgaccggca ccccgaacct gctgcggctg agcacgtcgg tcaacgtgac caagccgact
     1321 gtcactgacg cgatgcagga cgcgaacacc gtcatggccg ccgtccagca aaaattgaag
     1381 gcggacggcg tggcagcggc cgatctgcaa acgtcggggc tctcggtaca gccgaactac
     1441 tcctacgcga acaacactcc aaagctcacc ggataccagg tgagcgagaa cctgtcggtt
     1501 gtgctgcgcg acttgaatag ggcgggctcg atcatttccg atgcggcgca ggctggtggc
     1561 gacgcgttgc aaatcggtgg cgcgaccctc gacctcgatc aggacgacac actcatcgcg
     1621 caggctcgcc aggctgcatt cgctgacgca gaatccaagg cgaaggccta tgcaacagca
     1681 gcagggcgca cgttgggtgc ggtcacgagc atctcggaga ccactgactc gcagccgcag
     1741 cagttcgaca actacgcatt cgcggcggca gccccggcgg cctcaggtgc gcccgtcccg
     1801 atccaggctg gctcgcaaga cgtgaccgtg aacgtcgctg tgacgtgggc cctgaactga
     1861 tcagccgccg gcgcggcgcc gcaacgtgcg ctt
//
LOCUS       DASWVF010000728         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_224502, whole genome shotgun sequence.
ACCESSION   DASWVF010000728 DASWVF010000000
VERSION     DASWVF010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224502"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2074)
                     /locus_tag="VGD55_12820"
     CDS             complement(74..2074)
                     /locus_tag="VGD55_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871275.1"
                     /translation="MTRTTSTTNHRDRQLRRLRVLAAALLGGAGIAVAPVGAAVATPP
                     PPPSVCPDASGATFGPNVCVFNDTMAQSAIQHDLDAISTQQVPLSAQFDSNRYAILFE
                     PGTYGSATAPLIFQVGYYTEVAGLGYLPSDTTINGLIESFANDCGNRLNGNPPVLQFW
                     CNSTTNFWRSLSNLTLNEVTNDDTPGPPNYAPPVVDPYGPYCTNSEESWSVSQGAPIR
                     RAIINGSVVFQDYCAQGSSSNPSRDDASGGFIADSQVNGDLNFYGQQQYVTRNTQIQG
                     ANGCGTGGLWNMVYSGVTGATITPEFGGQCEQNTVLPTSPVTEETPFLYQDSRGAWQA
                     FVPAVQRNSAGPSWSSGAEAGSSIPLARFFVANPSTPVLRIDLALALGQNLILEPGIY
                     NLDSPIVVSRPNTVVLGLGFATLIPQRGSAALVAVANTGVKISGLLIDGGPVNSPVLL
                     SVGTPGPSSASNPDLISDVFFAIGGAQLGKADVSFLDLASNSIVDDLWAWRADHGNPG
                     TVGWTTNTAATGVVVDASNVTAYGLAVEHFQKNEVVWNGQGGTVVFYQNELPYDPPSQ
                     AAWMATPTQNGYPAFYVAPSIKRFNGYGMGSYVVFINTAATLYDAEAFEAPHTVGVQF
                     HNTFGVWIDGSGGLSSIINGVGGPVTSTNPGIVEPVDVPNYP"
BASE COUNT          399 a          655 c          741 g          430 t
ORIGIN      
        1 catggacggc cagtgaaaga gccgggctcc acgcccggct ccttcacgtt cacggcgatt
       61 actgcgacaa gccctacggg tagttcggga cgtcgacggg ctcgacgatt cccggattcg
      121 tcgaggtcac gggaccacca acaccgttga tgatcgagct aagaccgccc gatccgtcga
      181 tccagacacc gaacgtgtta tggaattgca cacccacggt gtgtggagct tcgaacgcct
      241 cggcgtcgta gagcgttgcc gcggtgttga taaagacgac gtagctgccc atgccgtacc
      301 cgttgaatct cttgatgctc ggcgcgacgt aaaaggcggg gtacccgttt tgggttggcg
      361 tcgccatcca ggctgcctgg ctcggcgggt cgtacggcaa ctcgttctgg tagaagacca
      421 ccgtcccgcc ttggccgttc cagaccacct cgttcttctg gaagtgctcg acggccaagc
      481 cgtacgccgt cacgttgctc gcgtcgacga cgacacccgt ggcggcagta ttcgtcgtcc
      541 agccaacggt cccagggttg ccgtgatcgg ctcgccacgc ccacaggtca tcgacgatcg
      601 agttcgaagc gagatcgagg aagctcacgt ctgcctttcc gagttgagct ccaccgatcg
      661 cgaagaacac gtcagaaatg aggtcggggt tggaggcaga gcttggacca ggcgtgccga
      721 ccgacaggag tactggtgag ttgaccggcc cgccgtcgat aaggagtccg gaaatcttga
      781 cgccggtgtt cgcaacggcg accaaggcgg cgcttcctct ttgcgggatg agagtcgcaa
      841 agccgaggcc gagaaccact gtgttcggac ggctgaccac gatcgggctg tcgaggttgt
      901 agatgccggg ctcgaggatc agattctggc cgagcgccag cgcgaggtcg atcctgagta
      961 ccggcgtact cgggttggcg acgaagaatc tcgcgagcgg gatggaagag cccgcctcag
     1021 cgccactcga ccacgatgga cccgccgagt ttcgctgcac ggcaggaaca aaggcctgcc
     1081 acgcgcctct ggaatcctga tacaggaacg gtgtttcttc ggtgaccggg ctcgtcggca
     1141 gcaccgtgtt ctgctcgcat tggccaccaa actccggcgt aatggtcgcg ccggtaaccc
     1201 cggagtagac catgttccac aggccgccag ttccgcatcc attggcgcct tggatctggg
     1261 tgttgcgggt gacgtattgc tgctggccgt agaagttgag gtctccattg acctgactgt
     1321 cggcgatgaa gccgccgctc gcgtcgtcac gtgacggatt gctgctcgag ccctgcgcgc
     1381 agtagtcctg gaagaccacg ctgccgttga tgatcgctcg ccggatcggt gcgccttgag
     1441 agaccgacca gctttcttcg ctgttggtgc agtaggggcc gtaagggtcg accaccgggg
     1501 gtgcgtagtt cggcggccca ggagtgtcgt cgttagtgac ttcgttcagg gtcaggtttg
     1561 acagtgatcg ccagaagttg gtcgtggagt tgcaccagaa ctgcagcacc ggcggattcc
     1621 cgttcaagcg gttcccgcaa tcgttagcga acgattcgat gaggccgttg atggtggtat
     1681 cgctggggag gtacccaaga cccgcaacct ccgtgtagta gccgacttgg aagatcagcg
     1741 gcgctgtcgc cgagccgtat gttccgggct caaagaggat tgcgtaacgg ttgctgtcga
     1801 actgcgccga gagcggtacc tgctgtgtcg agatggcgtc gaggtcgtgc tgaatcgcgc
     1861 tctgcgccat ggtgtcgttg aacacgcaga cgttcggacc gaacgtcgca ccgctggcgt
     1921 cgggacagac cgatggtggc ggaggtgggg tcgcgaccgc tgcgccgaca ggcgcgaccg
     1981 cgatgccggc gcccccgagg agcgccgccg caagaacacg gagccgccgg agctggcgat
     2041 ctcgatggtt cgtggttgat gtcgttcgtg tcacggatat gcgcctgcct ttcggtgagt
     2101 cgccgatcgc cggaagcggt tccggcgctg ggacagtaac tcccggaacc ggttccgtaa
     2161 agaccgtcag cccgcgtcgt acgacgtcag gtggctggtc gtgcgcaaca ttcgacgcag
     2221 gtcgg
//
LOCUS       DASWVF010000729         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_198760, whole genome shotgun sequence.
ACCESSION   DASWVF010000729 DASWVF010000000
VERSION     DASWVF010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198760"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..831
                     /gene="rph"
                     /locus_tag="VGD55_12825"
     CDS             100..831
                     /gene="rph"
                     /locus_tag="VGD55_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0871276.1"
                     /translation="MPTTSRLDGRANDELRSVTITRNWLDHAEGSVLVEFGRTRVLCA
                     ASVTEGVPRWRKGSGLGWVTGEYAMLPRATTTRNERESVKGRLGGRTQEISRLVGRAL
                     RACVDYKALGEATIQLDCDVLQADGGTRTAAITGAYVALYDAIEWLRKNGKMPGEPLV
                     RAVSAVSVGVIDGEPRLDLNYEEDVRADTDMNVVVTGDGEFIEVQGTAERDPLRRAEI
                     DALLDLAVGGCARLDALQRAALGIV"
     gene            842..1456
                     /gene="rdgB"
                     /locus_tag="VGD55_12830"
     CDS             842..1456
                     /gene="rdgB"
                     /locus_tag="VGD55_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0871277.1"
                     /translation="MTRVVLATRNAHKVIELRRILTAAGLDVDLVGMDAYPHVPEVPE
                     TGATFEDNALLKARAVARATGEIALANDSGLAVNALNGMPGVLSARWAGRPGSDSDEA
                     NLRLVLAQIADVPDQRLGAWFECAAAAVFPDDREFVVRGRLAGSITRIPRGSNGFGYD
                     PIFIPAGGDRTTAELGDDEKDAISHRGVAFRALAAQLQGLIEPR"
BASE COUNT          268 a          491 c          550 g          266 t
ORIGIN      
        1 cggcctcccg caactttgcg gggcccgcga ccttgacgca tcccggcgct gtgtacgacc
       61 tgtcaactgc tgatgggcgc gccggatagg gtcgagccca tgcccactac gagccggctc
      121 gatggccgcg cgaacgacga actgcgatcc gtcaccatca cccgcaactg gctcgaccac
      181 gccgaaggct cggtgctcgt cgagttcggt cgcacccgcg tgttgtgcgc ggcgagtgtc
      241 accgagggtg tgccgcgatg gcgcaagggc agcggccttg ggtgggtgac cggtgagtac
      301 gcgatgctgc cgcgggctac gacgacgcgc aacgagcgcg aatcggtgaa gggccggctc
      361 ggtggtcgta cgcaggagat ctcccggttg gtcgggcgcg cgctgcgtgc ctgcgtcgat
      421 tacaaggctc tcggcgaggc gacgatccaa ctcgactgcg atgtcttgca ggccgacggc
      481 ggcacccgca cggcggcgat caccggtgct tacgtcgctc tctacgacgc gatcgagtgg
      541 ctacgaaaga acgggaagat gcctggcgaa ccgctggtgc gcgcagtgtc ggccgtgagc
      601 gtcggcgtga tcgatggcga accaagactc gatctgaact atgaagaaga cgtgcgggcc
      661 gacaccgaca tgaacgtcgt cgtgacaggc gatggcgagt tcatcgaagt gcaaggtacg
      721 gctgagcgcg acccgctgcg cagagccgaa atcgatgcgt tgctcgatct cgcggtcggc
      781 ggttgtgcgc gcctcgacgc tcttcaacgg gcggccttgg ggatcgtgtg acggggatct
      841 cgtgacgcgg gtcgtgctcg ccacccgcaa cgcgcacaag gtcatcgagt tgcggcgcat
      901 cctcaccgcc gctgggcttg atgtcgacct ggtcggcatg gacgcatatc cacacgtacc
      961 cgaagtgccc gagaccggcg cgacgttcga agacaacgcg ctgttgaagg ccagggccgt
     1021 cgcgcgcgcg accggtgaga tcgcattggc aaacgattca ggtctggccg tcaatgcgct
     1081 caacggcatg cccggcgtgc tctcggcacg gtgggcgggt aggcccggca gcgacagtga
     1141 cgaagctaac ctgcgtctgg tgcttgccca aatcgccgac gtccccgacc agagattggg
     1201 cgcgtggttc gagtgcgccg cagcagcagt ctttcccgac gatcgcgagt tcgtcgtccg
     1261 cggacggttg gcaggctcca tcacccgaat cccgaggggg tcgaacggat ttgggtacga
     1321 cccgatcttc attccggccg gcggcgaccg cacgacggcg gagctaggtg atgacgagaa
     1381 agacgcgatc agtcaccgcg gggtcgcgtt ccgggcgctc gccgcgcagc tgcaggggct
     1441 gatcgagccc cgctgatcgg cgtcccgcat aaatcttttt cggccgcatg tatcagcgcg
     1501 tggtgtcatc gctcttttgt ggtgaccgcc gctcgggggg acgaccggtc aaatcgatgc
     1561 gagtcgcaca acagg
//
LOCUS       DASWVF010000730         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_224581, whole genome shotgun sequence.
ACCESSION   DASWVF010000730 DASWVF010000000
VERSION     DASWVF010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224581"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGD55_12835"
     CDS             complement(<1..765)
                     /locus_tag="VGD55_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY0871278.1"
                     /translation="MALARAWSVALAGVQGTVVEVEADLAPGLPGLTIVGLPDAALSE
                     SRDRVRAAVQNSAEPWPQKRITLGLSPATLPKHGSGFDLSMAVAVLCAAGGLRAKSLE
                     GVVFFGELGLDGRVRPIRGVLPATMAAAAAGFGRVVVPRANGAEAALVPDIEVWPVRS
                     LPELLAALRSGEPPECLVDDAVVADPEADRDELFDVELDLADVLGQPSGRRAVEVAAA
                     GGHHTLLLGDPGTGKTMLAARLPGLLPELDRAAALEV"
     gene            complement(765..1187)
                     /locus_tag="VGD55_12840"
     CDS             complement(765..1187)
                     /locus_tag="VGD55_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0871279.1"
                     /translation="MVGHFEVDAAAFGSGERAALGRYGEIVAADHLVASGMTVLARNW
                     RCREGEVDIVALDGDVLVMCEVKTRRGVGFGTPLDAVTPVKAARLRRLALRWLAEQRA
                     GVVEGTVVARYAEIRFDVISVLRPLSGPTVVDHLRGAF"
     gene            complement(1331..>1558)
                     /locus_tag="VGD55_12845"
     CDS             complement(1331..>1558)
                     /locus_tag="VGD55_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0871280.1"
                     /translation="AAADDLAGNLNPVGRIYYNFSTFLCVPNALSQAGGYALGAQAGE
                     AAIRRVVTEAGFTRFRRATETPFNIVYEARP"
BASE COUNT          284 a          577 c          503 g          194 t
ORIGIN      
        1 cacttcaagc gccgccgcgc gatcgagctc gggcaacagc ccgggcagcc gggcggcgag
       61 catggtcttg ccggtgccgg gatcgccgag cagcaaggtg tggtgccctc ccgcggctgc
      121 aacctcgacg gctcgacgcc ctgaaggctg gccgaggacg tcggcaagat cgagctcgac
      181 gtcgaaaagc tcgtcacggt cggcttccgg gtcagccacc actgcgtcat cgacgagaca
      241 ctcaggtggc tcgccactgc gcagcgcggc gaggagctca ggcagcgagc ggaccggcca
      301 cacttcgata tcgggcacga gcgcggcctc ggcgccgttc gcccgcggca ccacgactct
      361 gccgaacccg gccgccgccg cagccatcgt ggcgggcaga acgccgcgaa tcggccggac
      421 ccggccgtcg agaccgagct cgccgaagaa cacgactccc tcgagcgact tggctcgcaa
      481 gccgccggcc gcgcacaaca ctgcgaccgc catcgacagg tcgaacccgc tgccgtgctt
      541 gggcagcgtc gcgggcgata gcccgagggt gatccgcttc tgcggccatg gttccgctga
      601 gttctgcacg gcggcgcgga cccgatcacg cgactcagac agggcagcgt cgggcaaccc
      661 gacgatcgtc aagccaggca ggccaggcgc gagatcggct tcgacctcga cgaccgtgcc
      721 ttgcacgccg gcaagtgcca ccgaccacgc gcgagcaagc gccatcaaaa ggctccgcgc
      781 agatgatcga cgaccgtcgg acccgaaagc ggccgcagca ccgagatgac gtcgaaacga
      841 atctcggcat agcgcgcgac caccgtcccc tcgaccacac ccgcccgttg ctcggcgagc
      901 caccgaagcg cgagccgccg caaccgcgcg gccttgaccg gagtgaccgc gtcaagtggc
      961 gtgccgaaac cgacgcccct acgcgtcttc acctcacaca tcaccaacac gtcgccgtcg
     1021 agcgcgacga tgtcgacctc gccctcgcgg cagcgccaat tgcgtgccaa cacggtcatg
     1081 cctgacgcga ctaggtgatc agccgcgacg atctcgccgt agcggccaag cgccgcgcgt
     1141 tcaccggaac cgaacgccgc ggcgtccacc tcgaaatgcc ccaccaccca caccacctcc
     1201 gacgtccaca gtggcggtga ccgcgacgcc gcaagggccc gcgcacacct tctgtgcacg
     1261 ggccctgcga agcgatgaac ctgtggacga cgatcagccg gcgtccgacg ccagccaacc
     1321 gtcgtcccga ttacgggcgt gcttcgtaga cgatgttgaa cggtgtttcg gtcgcccgac
     1381 ggaaccgggt gaagcctgcc tcggtgacga cgcgccggat cgccgcttcg cccgcctgcg
     1441 cgcccaaggc gtagccgccc gcctgcgaga gcgcgttcgg cacgcaaaga aacgtggaga
     1501 agttgtagta gatgcgaccg accgggttga gattgccggc caggtcgtcg gcggcggc
//
LOCUS       DASWVF010000731         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_122289, whole genome shotgun sequence.
ACCESSION   DASWVF010000731 DASWVF010000000
VERSION     DASWVF010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122289"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VGD55_12850"
     CDS             <1..872
                     /locus_tag="VGD55_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871281.1"
                     /translation="QLEEKRKAAGFTKPLVVQYVEYLKQVFTLNLGTSITDNQPITTV
                     LKVNGAATLELSVAASLVALVIGVPIGLRAGRLRDGAFDAGGRLFGIITYAAPVFFVG
                     LLFQLVFSKWLNWLPNSGQLSPIDQAELPAHTHFSVIDAIWDHNGSAFVDALKHLAMP
                     AFTLGLLLCGVVIRLIRTNLIQTLKGDYVEAARARGISEHKVVMRHAFRNALVPVVTV
                     MGLQAALLLSGAVLTEETFNWPGIGNQLVTYLNNRDYIAVQGIITIFALAVVFISLLI
                     DFINAGIDPRVRY"
     gene            872..1864
                     /locus_tag="VGD55_12855"
     CDS             872..1864
                     /locus_tag="VGD55_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871282.1"
                     /translation="MSGERRRGPVSRLWEQVKQAQGFSKWLLWVGAAITLAFVVLAVF
                     APLIAPYGFDQYTSHGVRFPKQGHPNGMHLFGTNVRSVDVLSVVTYGSRTAIEVVLLA
                     VVFSLVVGVPLGMISGYYGGWLDRVLVLIMDALFAFPYLLLAIIIAFLLQNSVGSGVL
                     TAAIAITVIYVPQYFRVVRGQVLSAREEPYVEAARALGARPRTIMTRYLFFNVIQSVP
                     VIATLNAADAILTLAGLGFLGFGIQPTQAAEWGYELQRGVADMGAGIWWTSVFPGGAI
                     VLLVTGLTLVGEGLNDVLNPVLRKRAGRRVTLPARSLAESLITATPTATAEELP"
     gene            1861..2889
                     /locus_tag="VGD55_12860"
     CDS             1861..2889
                     /locus_tag="VGD55_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871283.1"
                     /translation="MNVTPVISVRDMRVWYGTERGPVRAVDGVSFDVRPGETLGLVGE
                     SGCGKSTLGRGILGLLPTGSAIDGSVRFRDRELVGVRQSEMREMRGAELGLIFQEPMT
                     RLDPLMRISEHFAETLRAHEPELNSRQIDARSIDTLRRMGIPPTRFRHYPHEFSGGMR
                     QRIMIALALVLNPAFVVADEPTTALDVLVEAQILAILHDLKRNFDTSLLLITHNLGIV
                     AEACERVAVMYAGKIVEEGSVQQVFAAPAHPYTRELLRSTISLSTTALHYIPGSPPDL
                     VNPPSGCHFNPRCPSAMQVCPTLDPVEVVATSGQRVACWLHGPSQLVPPGGTTPLPRQ
                     ELASADEA"
     gene            2873..4234
                     /locus_tag="VGD55_12865"
     CDS             2873..4234
                     /locus_tag="VGD55_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871284.1"
                     /translation="MPTKPNDLVDDAGSVGAQERPVVLDVRDLKVHFPLRGGFLDRLL
                     GRSSGSVKAVDGISFTLHKGEVLGLVGESGSGKTTTARALLGLAPITAGSVRFDPGER
                     FGGPTELTGLGERQFRALRQAIQLVFQDPHASLNPAMDLATAVAHPLKMHKITKDRAE
                     IDRRVREAFERVGLAPVDQYLHKYPADLSGGQKQRAVLARAIMLNPALLVADEPVSML
                     DMSVRAKILQLMLDLKADLGLTYLYVTHDLATAKFFCDTIAIMYLGRIVEIGPAASIF
                     ADPKHPYTRSLLAAIPEPDPSLTRARVLPRGEIPDAAVPPLGCAFHPRCPAAFAPCGW
                     ESRDLQALLEQRWTEVAEDEYARERAEIGDPVALDAGMQARLPDKRPGSGAAAALLDQ
                     IRVADPDEPFWSGVRQLASEPDGVRIEFDEPVDPVLRDVGDVHVACHLHDPRFAADSR
                     PAS"
     gene            4275..>4853
                     /locus_tag="VGD55_12870"
     CDS             4275..>4853
                     /locus_tag="VGD55_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0871285.1"
                     /translation="MESERFVGERLHSHGGRGVSLALVSAISFGVAGPFSKAVLTSGE
                     LSPLRLAQFRITTAALVMLAVVAVRHLNGTRPTHRWTLADAWLVIAYGSLGFVAVQIC
                     YFIAIDRLPVGVALLFEYMSVVLVAVYAAVLQHRPQPRAVWAGVTLAVTGVVVLTQPW
                     SGFQLNVWGSVAGAGAASGCAAYFLIGERGTAR"
BASE COUNT          746 a         1565 c         1609 g          933 t
ORIGIN      
        1 ctcagctcga agaaaagcgc aaggccgccg gcttcaccaa accgctcgtg gtgcagtacg
       61 tcgaatacct caagcaggtg ttcaccctca atctcgggac gtcgatcacc gacaaccagc
      121 ccatcaccac ggtcttgaag gtgaacggcg cggcaacgct tgagctatcg gtggcggcga
      181 gcctggtggc gttggtcatc ggcgtcccta tcggcttgcg cgcgggccgc ttgcgcgacg
      241 gcgcgttcga cgcaggcggc cggttgttcg gcatcatcac ctacgcggcc ccggtcttct
      301 tcgtcggcct gttgttccag ctcgtgttca gcaagtggct gaactggttg ccgaactcgg
      361 gacaactgag ccccatcgat caggcagagt tgccggccca cacgcacttc agcgtgattg
      421 acgcgatctg ggatcacaac ggcagcgcgt tcgtcgacgc gctgaaacac ttggcgatgc
      481 cggccttcac gctgggcctg ttgctgtgcg gcgtcgtcat caggctgatc cgcaccaact
      541 tgatccagac gctgaagggc gactacgtcg aggctgctcg ggcgcggggt atctcggagc
      601 acaaggtcgt catgcgccac gcgtttcgca acgcgctcgt gccggtggtc actgtgatgg
      661 gcttgcaggc agcactcttg ctgtcgggtg ctgtgctgac cgaagagaca ttcaactggc
      721 cgggcatcgg caaccagctc gtcacctacc tcaacaaccg cgactacatc gcggtgcagg
      781 gcatcatcac gatcttcgcg ctcgcggttg tgttcatcag cctgctcatc gacttcatca
      841 atgccggcat cgatcccagg gtgcgttact gatgagtggc gagcggcggc gcggcccggt
      901 cagccggctg tgggaacagg tcaagcaggc gcagggattc tcgaagtggc tgctgtgggt
      961 cggcgccgcg atcaccctcg ccttcgtggt tctcgccgtc tttgctccac tgattgcgcc
     1021 gtacggcttc gaccagtaca cgagccacgg cgtgcggttc ccgaagcagg ggcacccgaa
     1081 tggcatgcac ttgttcggca ccaacgtgcg ctcggtcgac gtgctgtcgg tcgttacata
     1141 cggctcgcgc acggccatcg aggtcgtgct gctcgcggtg gtgttcagcc tcgtcgtcgg
     1201 cgtgccactc ggcatgatct cgggttacta cggcggctgg ctcgaccggg tgctcgtgct
     1261 gatcatggac gcgctcttcg cgttccccta cctgctgctc gcgatcatca tcgccttctt
     1321 gttgcagaac agcgtgggca gtggcgtact caccgcggca attgcgatca cggtcatcta
     1381 cgtgccgcag tacttccgtg tcgttcgggg ccaagtgctg tcggctcgag aagagcccta
     1441 cgtcgaggcg gctcgcgcac tcggtgcgcg cccgcgcacg atcatgaccc ggtacttgtt
     1501 cttcaacgtg atccagtcgg tgcctgtcat cgccaccctg aacgccgcgg acgcgatcct
     1561 gacgctcgcc ggcttgggtt ttctcgggtt cggcatccag ccgacgcagg ccgcagagtg
     1621 gggttatgaa ctccagcgcg gggtagccga catgggcgcc ggcatctggt ggaccagcgt
     1681 cttccccggc ggcgcgatcg tcttgctggt caccggtctg acgcttgtcg gtgaaggcct
     1741 gaacgacgtg ttgaacccgg tgctcagaaa gcgggcggga cgccgagtga cgctgcccgc
     1801 gcgcagtctt gcggagtcac tcatcaccgc gaccccgact gctacggcgg aggagttgcc
     1861 gtgaacgtga ctcctgtcat ctccgtgcgt gacatgcgcg tttggtacgg caccgagcgc
     1921 gggcccgttc gcgccgtcga tggcgtctcg ttcgacgtgc ggcctggtga gactctcggc
     1981 ctcgtgggcg agtcgggttg cggtaagtcg acgctcggac gcgggatcct cggtctgctg
     2041 ccgaccggtt ctgcgatcga tgggagtgtg cgcttccgtg atcgcgaact ggtcggcgtc
     2101 cggcaaagtg aaatgcgaga gatgcgcggg gccgagctcg gcctgatctt ccaggaaccg
     2161 atgaccaggc ttgatccgct gatgcgcatc agcgagcatt tcgcggagac gctgcgagcg
     2221 cacgagccgg agctgaattc ccgccagatc gatgcgcgat cgatcgacac gctgcgccgc
     2281 atgggaattc cgccgacccg tttccgccac tacccgcacg agttctccgg tggcatgcgg
     2341 caacgcatca tgattgcgct cgcgctcgtg ctgaatcccg cgttcgtcgt cgccgacgag
     2401 ccgacgaccg cgctcgacgt gttggtcgaa gcgcagatcc tggcgatcct gcacgacctg
     2461 aagcgcaact tcgacacgtc gctcctgctg atcacgcaca acctcgggat tgtggccgag
     2521 gcatgcgagc gggtcgcggt gatgtacgcc ggcaagatcg tcgaagaggg ctcggtacag
     2581 caggtattcg cagctccggc gcacccgtat acgcgcgagt tgctccgatc gactatctct
     2641 ctctcgacga ccgcactgca ttacatcccg ggctcaccgc ccgatctggt caatccgccc
     2701 agcggctgcc acttcaaccc gcggtgtccg agcgcgatgc aggtgtgccc aacgctcgat
     2761 ccggttgagg tcgtcgcgac ttccggacag cgggtggcgt gctggctgca cggtccgtct
     2821 caactggtcc cgcccggtgg cacgacgccg ctgccacgac aggagctcgc cagtgccgac
     2881 gaagcctaac gatctcgtcg acgacgccgg ttccgtcggc gcacaagagc ggcccgtcgt
     2941 gctcgacgtc cgcgacttga aggtgcattt ccctttgcgt ggtggattcc tcgaccgcct
     3001 gctcggtcgt tcgtcgggtt cggtgaaggc cgtcgacggc atctctttca cgttgcataa
     3061 gggcgaggtg ctcgggcttg tcggtgagtc cggcagtggc aagacgacca ccgcgcgtgc
     3121 gctgctcggc ctcgcgccga ttaccgcagg cagcgtcaga ttcgatcccg gtgagcgatt
     3181 cggcgggccg accgaactca caggtctcgg tgagcgacag ttccgcgcac tgcgccaagc
     3241 tatacagctc gttttccagg atccgcacgc gtcgctgaat ccggccatgg acctcgccac
     3301 cgccgttgcg catccgctga agatgcacaa gatcaccaag gatcgggccg agatagaccg
     3361 gcgggtacgt gaagcgttcg agcgcgtggg tcttgcaccg gtcgaccagt acctgcacaa
     3421 gtacccggcc gacctctcgg gcggtcagaa acagcgcgcg gtgttggcgc gcgcgatcat
     3481 gctgaacccg gcgctgctcg tcgctgacga accggtatcg atgctcgaca tgagtgtgcg
     3541 cgccaagatc ttgcagttga tgctcgatct gaaggccgac ctcgggctca cgtatctgta
     3601 cgtcacccac gacctcgcga cggcgaaatt cttctgcgac accatcgcga tcatgtacct
     3661 cggccgcatc gttgagatcg gcccggcggc gagcatcttc gctgatccca agcacccgta
     3721 caccaggtcg ttgcttgccg cgattcccga acccgacccg agcctcaccc gtgcgcgggt
     3781 gcttccacgc ggggagatcc ccgacgccgc ggtgcctcca ctcggctgcg cgtttcaccc
     3841 gcgctgccca gcagcgttcg caccctgcgg ctgggagagc cgcgatttgc aggcgctgct
     3901 cgagcaacgc tggactgaag tcgccgaaga cgaatacgcg cgagagcgcg cagagatcgg
     3961 ggacccggtc gcgctcgacg cagggatgca ggctcggctg cctgacaaac gaccagggtc
     4021 cggcgcggcg gcggcattgc tcgaccagat ccgcgtcgcg gatcccgacg agccgttctg
     4081 gagtggggtg cggcagctgg cgtccgagcc agacggcgtc cgcatcgagt tcgacgagcc
     4141 ggtcgatccg gtgctgcgcg acgtcgggga cgtgcacgtc gcgtgccacc tgcatgatcc
     4201 gcggtttgct gcggacagtc ggcccgccag ctaattgctg cggactgcgt cagggacggc
     4261 gatagcctcg gcgggtggag agcgagcggt tcgtgggcga gcggttgcat tcgcatggcg
     4321 ggcgcggggt gtcgctcgcg ctcgtgagtg cgatctcgtt cggggtcgcc ggaccgttct
     4381 cgaaggcggt actgacgagc ggtgagctca gcccgctgcg gctcgcgcag ttccggatca
     4441 cgacggccgc gttggtcatg ctcgccgttg tggccgtgcg gcatctgaac ggcacgcgtc
     4501 ccacccatcg ctggactctg gccgatgcgt ggctggtgat cgcatacggc tcgctcggct
     4561 ttgtcgccgt tcagatctgc tacttcattg cgatcgatcg gctgccggtc ggcgtcgccc
     4621 tcctgttcga gtacatgtcg gtggtgttgg tcgcggtgta tgcggccgtg ctgcaacatc
     4681 gaccacagcc gcgtgcggtg tgggcgggcg ttacgctcgc cgtcaccggc gtggtggtgt
     4741 tgacgcagcc atggtcggga tttcagctga acgtgtgggg gtccgtcgcc ggcgccggcg
     4801 ctgcctcggg atgtgcggcg tacttcctga tcggcgaacg cgggacggcg cgg
//
LOCUS       DASWVF010000732         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_377068, whole genome shotgun sequence.
ACCESSION   DASWVF010000732 DASWVF010000000
VERSION     DASWVF010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377068"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1433
                     /gene="cobA"
                     /locus_tag="VGD55_12875"
     CDS             165..1433
                     /gene="cobA"
                     /locus_tag="VGD55_12875"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY0871286.1"
                     /translation="MNTGYPLLIDVAGRRVVVVGAGAVGARRAIGLAEAGARVDVVAP
                     TVDPSIEADDRITVIRRRYATADLDGAWLVHACTGDQRVDARVAADAEAARVWCVRAS
                     SAVSSAAWTPAVARVDGVVVAVNAGGDPDRAVAVRDAVRLLLQTGDLATRSVRRRARG
                     SVGSVGTVALVGGGPGDPDLITVRGRRLLANADVVVVDRLGPRALLAELAPDVEVIEA
                     GKAPDNHTLPQDAINSLLISLAQQGKRVVRLKGGDPFVFGRGGEEMLALVQAGVPCEI
                     VPGVTSAIAVPAAAGIPVTHRNLSRQFTVVTAHEDLDWPKLAAIEGTLVLLMGATRVE
                     RIAKELLSAGCDPATPVAVIENGTMPDQRTTTGSLDSIGDRATAVGVRAPAVVVVGDV
                     AALHELTHDFTHDRTHDLTTNPRSSTGGGT"
     gene            1430..2146
                     /locus_tag="VGD55_12880"
     CDS             1430..2146
                     /locus_tag="VGD55_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEY0871287.1"
                     /translation="MSEPALLAVAHGSADPRAEQVLDALIARVRSERPDVVAMIAHLG
                     FSDPDVPTALDSLARQGFREVVVVPLLLSPAYHATVDLPVALANAALDHPGLAIRQAA
                     VLGPHPLLFDLLRRRLADARVEPGAAVVLGAVGTSDATVNAALADVAATLGATIGFAS
                     GTPAIADVVTGLRAGGARQVAVATYVIAPGQLVDRLYDAGADVVTDVLGAAPELVDLV
                     LHRYDAALSATTVAGMLAAG"
     gene            2229..3020
                     /locus_tag="VGD55_12885"
     CDS             2229..3020
                     /locus_tag="VGD55_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEY0871288.1"
                     /translation="MTLSHKIVGNAMQLAVCQIADGQTIYADAGKFLWKTVNVSIETH
                     IASQASAAGGGGAGGGLLGMAMNAGKRMLAGESIAFQYFTARGGAGLVSVAGTLPGEM
                     RALELDGTKGWLAEKDSFVAAEGTVTNDIAFSGLRQGLKGGEGFVLQRFGGVGTVLIG
                     GAGNFIDINPAKYGGKIQVHTGCLVAFEDTLRYGVERVGALNAQTIVTGLLGGNGFNL
                     ATLEGDGNAILQSMTIDSLAHAITKHSHAGHDEKASNPLGSLFGG"
     gene            3030..3398
                     /locus_tag="VGD55_12890"
     CDS             3030..3398
                     /locus_tag="VGD55_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871289.1"
                     /translation="MGLLDKAKASATAAMAQGQAKVGAFQQGRSESELYKALGEAFFR
                     EQRQGGDHQATVDALVALDTHMAQAAAAAAAAAQPAPYQAPPAQGAPYQPPPGQPYTP
                     PPASGPPAATPGGNFTLDDM"
     gene            3485..>4014
                     /locus_tag="VGD55_12895"
     CDS             3485..>4014
                     /locus_tag="VGD55_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0871290.1"
                     /translation="MPEFIYVFRKARKAYGEKVILDDVTLSFLPGAKIGVVGPNGAGK
                     SSVLKIMAGIEQPSNGEAYMTPGFTVGILAQEPKLDDTKTVLGNVEDGVSETLALMAR
                     FNQVSEELGSPDADYDTLLAEMGELQAQLDHHNAWDLDAQLEQAMDALRCPPPDADVS
                     VLSGGERRRVALCKLLL"
BASE COUNT          623 a         1358 c         1363 g          670 t
ORIGIN      
        1 gacgggcaga cgctcgcggc gggtctcgtg ggtgacccca tcgcggcggc atcgtcgcgt
       61 gcagcttcgg gtgttgcttc gtcggatgcc gcatcgtcgg gtgccgcatc atcgtcgggt
      121 gccgcatcga ccagcggtgc cgcttcggcc ggtggcagga cgccgtgaac accggctacc
      181 cgctccttat cgacgtcgct ggacgacgag tcgtggtcgt cggagcggga gcggtcggag
      241 cacgccgggc tatcgggctc gctgaggcgg gggcgcgtgt cgacgttgta gcgccgaccg
      301 tcgacccgtc gatcgaggcc gatgaccgca tcacggtgat ccgccgtcgc tatgcgacgg
      361 cggatctcga cggcgcttgg ttggtacatg cctgcactgg cgaccaacgt gtcgacgcgc
      421 gggtggccgc cgacgccgag gctgctcgcg tgtggtgtgt acgtgcgtcg tcggctgtgt
      481 cgtcagccgc atggactccc gccgtcgcgc gcgtcgacgg tgtcgtcgtc gcggtgaatg
      541 cgggcggcga ccccgaccgc gcagtcgcgg tgcgcgacgc ggtgcgactg cttctccaga
      601 ccggcgatct cgcgacgcgc agcgttcgcc gtcgtgcgcg cggctctgtc ggctctgtcg
      661 gcaccgttgc gttggtcggc ggcggtcccg gcgaccccga cctcatcacg gtgcgcggcc
      721 gtcgactgct cgccaacgcc gatgtcgtgg tcgtcgatcg gttggggccg cgtgcgctgc
      781 tcgccgaact cgcgcccgac gtcgaggtga tcgaggccgg caaggctccc gacaatcaca
      841 cgctgccgca agatgccatc aacagcctgc tgatttccct tgcacagcaa ggcaaacgtg
      901 tcgtgcgact gaagggtggc gaccctttcg tgttcgggcg cggcggtgag gaaatgctcg
      961 cgctcgtgca ggccggtgtg ccatgcgaga ttgtgccggg agtgaccagc gcgatcgccg
     1021 tccccgccgc cgcggggatc cccgtcacac atcgcaacct ctcacgtcag ttcacggtgg
     1081 tcaccgcgca cgaggatctc gactggccga agctcgccgc gatcgagggc actttggtgc
     1141 tgttgatggg cgcgaccaga gtcgagcgca tcgccaagga gttgctgtcc gccggctgcg
     1201 atccggccac cccggtggcg gtcattgaaa acggcaccat gccagatcag cggacgacga
     1261 cgggctccct tgactcgatc ggcgaccgtg cgacggctgt cggtgtcaga gcgccggcgg
     1321 tcgtcgtcgt gggcgatgtt gccgcgctcc acgagctgac acacgacttc acgcacgacc
     1381 gcacgcacga cctcaccacg aatccgcgat cgagcacagg aggcgggaca tgagcgagcc
     1441 ggcgttgttg gcggtcgcgc acgggagtgc cgacccacga gctgagcagg tcctcgacgc
     1501 gctgatcgcg cgcgtgcggt cggagcgacc ggacgtcgtc gcgatgatcg cgcatcttgg
     1561 attctccgat cctgacgtgc cgacggccct tgattccctt gcgaggcaag gatttcgcga
     1621 ggtggtcgtc gtccccctgt tgttgagccc tgcgtaccac gccactgtcg acctgcccgt
     1681 tgcgctcgcg aacgcggcac tggatcatcc tggcctcgcg atccggcaag ctgccgttct
     1741 cggcccacat ccgctgctgt tcgacctcct tcggcgtcgc ctcgccgatg cccgggtcga
     1801 gcccggagca gccgtcgtcc tcggcgcggt cggcacctcc gacgccacgg tgaacgcagc
     1861 gcttgccgac gtcgcggcaa ctctcggcgc cacgatcgga ttcgcttcgg gcacgcctgc
     1921 gatcgccgac gtagtcaccg gcctgcgcgc cggcggcgcc cgccaggtcg cggtggccac
     1981 ctacgtcatc gcccccggcc agctggtcga ccgcctttac gacgcgggcg ccgacgtcgt
     2041 caccgacgtg ctcggcgcgg ctcctgagct cgtcgatctc gtcctgcacc gctacgacgc
     2101 cgcactgtcc gcgaccaccg tggcgggcat gctcgccgcc ggttagggcc ctacccgttt
     2161 gcggcgaaca tcggtagcgt cgtgctcgca cctgcgaggc acatcttcgg tggagggaac
     2221 cgggcgctat gactctttct cacaagatcg tcggcaatgc catgcagttg gctgtgtgcc
     2281 agattgccga cggccagacc atctatgcgg acgccgggaa gttcctctgg aagacggtca
     2341 acgtcagcat cgagacgcac atcgccagcc aggcttcggc cgctggaggc ggcggcgccg
     2401 gaggcggtct gctcggcatg gcgatgaacg caggcaagcg gatgctggca ggcgagtcga
     2461 tcgcgttcca gtacttcacc gcgcgcggcg gcgcgggcct ggtcagcgtc gcaggcacgc
     2521 ttccgggtga gatgcgcgca ctcgagctcg acggtactaa gggctggctc gccgagaagg
     2581 actcgttcgt ggcggccgaa ggcaccgtca ccaacgacat cgcgttcagc ggactgcggc
     2641 agggcctcaa gggcggtgag ggcttcgtcc tccagcgctt cggcggggtc ggcacggtgc
     2701 tcatcggcgg cgccggcaac ttcatcgaca tcaacccggc gaagtacggc ggcaagatcc
     2761 aggttcacac cgggtgcctg gtcgcgttcg aagacacgct gcgctacggc gtcgagcggg
     2821 tcggcgctct caacgcgcag acgatcgtca ccggcttgct cggtggcaac ggcttcaacc
     2881 tcgcgacgct cgagggagac ggcaatgcaa tactccagtc gatgacgatc gactcgctcg
     2941 cgcacgccat caccaagcac tcgcatgctg gccacgacga gaaggcaagc aatccgctcg
     3001 gcagtctgtt cggagggtag gaaacttcaa tgggacttct cgacaaggca aaggcgtcgg
     3061 caactgctgc gatggcgcag ggtcaggcga aggtcggcgc tttccagcag ggtcgctcag
     3121 aatctgagct gtacaaggca ctcggtgagg cgttcttccg cgagcagcgg caaggtggcg
     3181 accaccaggc caccgtcgac gcgcttgttg cgctcgacac gcacatggcg caggcagcag
     3241 cggctgcggc tgcggcagct caacctgctc cgtaccaggc gccgccagct cagggcgctc
     3301 cctaccagcc gccgcccggc cagccgtaca caccaccgcc cgcaagtggt ccgccagcag
     3361 cgactcccgg tgggaacttc accctcgacg acatgtagct cgcgccggtt ctgagaactc
     3421 ggtcggcaga ggctgacgcg tccgtggctc ggcggctgat cgacgtcccg tagacttctc
     3481 gaccgtgcct gagttcattt acgtcttcag aaaagcccgc aaggcctacg gcgagaaagt
     3541 cattctcgac gacgtcacct tgtcgttcct gcccggcgcc aagatcggcg tcgtcggtcc
     3601 caacggtgca ggcaagtcga gcgtgctcaa gatcatggcc ggcatcgagc agccgagcaa
     3661 cggcgaggcg tacatgaccc cgggcttcac cgtggggatc ctcgcgcagg agccgaagct
     3721 cgacgacacg aagacggttc ttggcaacgt cgaggacggc gtctctgaga cgctcgccct
     3781 gatggcgcgg ttcaaccagg tctcagagga gctcggcagt cctgacgccg actacgacac
     3841 gcttctcgcc gagatggggg agttgcaggc gcagctcgac caccacaacg cgtgggatct
     3901 cgacgcccaa ctcgagcagg cgatggatgc cctgcgctgc ccaccgcccg acgccgacgt
     3961 cagcgtgctg tcgggtggcg agcgccgccg ggtcgccctg tgcaagttgt tgct
//
LOCUS       DASWVF010000733         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_147841, whole genome shotgun sequence.
ACCESSION   DASWVF010000733 DASWVF010000000
VERSION     DASWVF010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147841"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..720
                     /gene="yajC"
                     /locus_tag="VGD55_12900"
     CDS             265..720
                     /gene="yajC"
                     /locus_tag="VGD55_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0871291.1"
                     /translation="MGIALPLLLFVLVYVVVILPQGRRRKQAAALQRAVEPGSKVILT
                     SGLHATVVEIDDDAIVVEAAPGVQLRYAKAAVLRVVPEPIDDEYDDDHDDAHDDHELE
                     GSGDTSHTGTADHDGDTVARPSNREDPSAGDDATGTSGNDAGSGPVPTS"
     gene            798..2591
                     /gene="secD"
                     /locus_tag="VGD55_12905"
     CDS             798..2591
                     /gene="secD"
                     /locus_tag="VGD55_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0871292.1"
                     /translation="MPPATRSSSGRPLTLVLLLLVLLYGGMALAGRYTPTLGLDLRGG
                     TTVTLKPTTTAGNKVKSSDLETAKNILLNRVNSLGVGNAEVVREGSNIVISLPGKNTQ
                     GALDNIGKTALLSIRQVYESNDPSMTGALVADTSATVTQSPAPATPAPSTSATPSSAA
                     APSTASQAPATSPKALGGGAPQAEPAGFVRAASSTAASAAAAPTPAAAAGSTAASPSA
                     AASPVAIPTVAETTPTAAELAAYQALDCTKDQQKVSGQEDIPTKFMVSCTDPQTSPAA
                     YKYLLMPTLIQGKEISSADATLIGTTGAVWGVNLTFKSAASSRWANFTAKNINKDTAV
                     VLDGLVTSAEYIAEAIPGGNTQISGNFTHKTASALANSLKYGALPLAFEPQTAYTVSA
                     SLGSSELKAGLLAGALGLVLVVLYSIFYYRGLSVVTVSSLALSAAIQYPIIVLLGKSI
                     NYTLTLAGIAGLIVAIGVTADSFVVFFERLRDEVRDGNSLRTSVEKGWVRARRTIVSA
                     DFVSIIAAVILYWLAIGDVRGFAFTLGLSTIIDLVVVFLFTKPLISLLAKTKFFGNGH
                     RLSGLDPGHLGVQKIAGTGTVVMRRAPARKV"
     gene            2593..3816
                     /gene="secF"
                     /locus_tag="VGD55_12910"
     CDS             2593..3816
                     /gene="secF"
                     /locus_tag="VGD55_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0871293.1"
                     /translation="MSRLGTLGHKLYTGQASYEFVGRWRRWLALSGVVILIGIIGLAT
                     RGLSLSLEFKGGTQFTFPDKGSSVAAVTKVVVDAGAPSPVVEKLSNAGLGVANYQVQT
                     KPLTTDEITKVTDAITSNLSVTKQQVSLDTVGSSWGSQITHKAVESLIIFLVAIVIYL
                     SLRYEWKMAAAAIIALIHDLIITTGIYALVGFDVSPSTVIGLLTILGYSLYDTVVVFD
                     KVRENTAPIAGGSRYTYAEAANLAVNQTLVRSINTSIIALLPVASLLFVGAGLLGGGS
                     LKDLALALFVGLATGSYSSIFIATPLLVLFKEREPQYIALTKRVSARVGSSTRAANVV
                     AAAAAGAAATSGGGISTAVADRPAPIDVDNTPETVDPPAAGTAVTRAPTPPVRRPGTP
                     PRRQGGRPSGKKRRR"
     gene            3813..4355
                     /locus_tag="VGD55_12915"
     CDS             3813..4355
                     /locus_tag="VGD55_12915"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0871294.1"
                     /translation="MTAGEVEELIRGLVRDVPDYPSPGILFKDITPLLADAAAFAAIV
                     DALVEQHAPGSIDKVVGIEARGFILAAPVAYHLNAGFVPMRKKGKLPYQTFETSYALE
                     YASATIEVHRDAFAPGDRVLIVDDVLATGGTALAALDLVHRAGGTPVGFSFLLELRAL
                     AGRAALAGAGEGHIRSLFIV"
     gene            4431..>5286
                     /locus_tag="VGD55_12920"
     CDS             4431..>5286
                     /locus_tag="VGD55_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0871295.1"
                     /translation="MPSRAVPERVANALDEPAPDMPGVTELAGVVAVAADAEPVEEAE
                     LIAERELAAAAADGSEGPDSAEGSDGGDGGDDANEQSAPSSGRRVRARLARLATPRTS
                     TTTSPVLEPLIRSIRLTHPKADTRAIQRAYDVAEHHHRLQFRKSGDPYITHPLAVATI
                     LAELGMTPPTIVAALLHDTVEDTEYTLETLRTDFGDEVAALVDGVTKLDRVKYGDAAT
                     AETVRKMVVAMARDPRVLVLKLADRLHNMRTLRYLPAEKQERKARETLEIYAPLAHRL
                     GMNTLKWEL"
BASE COUNT          912 a         1767 c         1719 g          888 t
ORIGIN      
        1 acggcgaccg cggcggcgac gctattcgag ggactcgaag atgttgggcg cgagggactc
       61 gaagatgttg ggcgcgaggg actcgaaggc gtcgagccgg aggcagggga ggtcgaggcg
      121 ctcgagagcc tcgagggcgg ggcatagccg atcgcataga ctgcgccgcg gccgagggct
      181 agtgccgcgc gctctttcga tccaccccgc ccaggtcgcc gcggaccttc cgccggcagt
      241 tccggttcgt tggaaggaaa gctcgtgggt atcgctctcc cgttgctgct cttcgtcctg
      301 gtctacgtgg tggtgatctt gccccagggc cggcggcgca agcaggctgc ggcgctccag
      361 cgcgcggtcg agccgggcag caaggtgatt ctcacgtccg gtcttcacgc cactgtggtc
      421 gagatcgatg acgacgccat cgtggtcgag gcggcgccgg gtgtgcagct gcggtacgcg
      481 aaggcggcgg tgctgcgggt ggtccccgag ccgatcgacg acgaatatga cgatgaccac
      541 gatgacgccc acgacgacca cgagttggaa gggtccggcg ataccagcca cactgggaca
      601 gcagaccatg atggagacac cgtcgcccgg ccgtcgaatc gcgaggatcc gtcggcgggg
      661 gacgatgcga cgggaacgag tgggaacgat gcaggatctg ggcccgttcc cacgtcctga
      721 ccggcgtcgg cgatacttcg tgcgctgcga gaatgcgctg cgagactaaa ttctggtgag
      781 acgacaggga gaaacaggtg ccacctgcca caaggtcgag ttcagggcgt cccctgacct
      841 tggtccttct tcttctcgtc ctgctctatg gcgggatggc gctcgccggc cgttataccc
      901 cgacgctcgg acttgacctg cgtggcggca cgaccgtcac cttgaagccg acgaccaccg
      961 ccggcaacaa ggtcaagagc tccgacctcg agaccgccaa gaacatcctg ctgaaccggg
     1021 tcaatagcct cggtgtcggc aacgccgaag tggtacgcga gggctccaac atcgtgatct
     1081 cgctgccggg caagaacacc cagggcgcgc tcgacaacat cgggaagacc gccctgctga
     1141 gtatcaggca ggtctacgaa tcgaacgatc cgtcgatgac gggcgcgttg gtggccgata
     1201 cctccgcgac agtgacgcaa tcgcctgccc ccgcgacgcc ggcaccttcg acttcggcca
     1261 cgccttcaag cgcagccgcc ccctcgacgg catcccaggc accagccacc agcccgaagg
     1321 cgctcggcgg cggcgcacca caagccgaac ccgcgggctt cgtccgggcg gccagcagca
     1381 cggcggcgag cgccgcggcc gcgccaacgc cggccgccgc agcgggaagt acggcagcga
     1441 gcccctcagc cgctgccagc ccggtcgcta tccccacggt tgctgagacg acgccgaccg
     1501 ctgccgagct agcggcctac caggcgctcg actgcacgaa ggatcagcag aaggtgtcgg
     1561 gccaagaaga catccccacc aagttcatgg tctcgtgcac tgacccgcag acgtcgcccg
     1621 cggcgtacaa gtacctgctg atgccgacgc tgatccaggg caaggagatc agcagcgctg
     1681 acgccaccct gatcggcacc accggtgcgg tctggggcgt caacctgacc ttcaagagcg
     1741 ccgcgtcgtc tcgctgggcg aacttcaccg cgaagaacat caacaaggac accgccgtcg
     1801 tgctcgacgg acttgtgacg tctgcggaat acatcgccga agcgatcccc ggcggcaata
     1861 cgcagatcag tggaaacttc acgcacaaga ccgccagcgc gctcgcgaac tcgctcaaat
     1921 acggcgcgct cccgctggcg ttcgagccac agaccgccta cacggtgtcg gcgtcacttg
     1981 gcagcagcga actcaaggca ggcctgcttg cgggcgccct cggactggtc cttgtcgtgc
     2041 tgtattcgat cttctattac cgaggtcttt cggtcgtcac ggtgagcagt ctcgcgctgt
     2101 cggcggccat ccagtacccg atcatcgtgt tgctcggtaa gtcgatcaac tacaccctga
     2161 cgctcgccgg catcgccggt ctcatcgtcg cgatcggtgt gacggctgac tcctttgtcg
     2221 tcttcttcga acgactgcga gacgaagtgc gcgacggtaa ctcgctgcgt acctcggtcg
     2281 aaaagggctg ggtgcgcgcg cggcgcacga tcgtgtcggc tgacttcgtg tcgatcatcg
     2341 cggccgtgat cttgtactgg ctcgcgatcg gtgacgtgcg tgggttcgcc ttcaccctcg
     2401 ggctctcgac gatcatcgac ctcgtggtgg tcttcttatt caccaagccg ctgatcagcc
     2461 tgctggcaaa gacgaagttc ttcggcaacg gccatcgcct gtccggactc gatccggggc
     2521 acctcggcgt ccagaaaatt gctggaaccg gaactgtggt catgcggcgc gctccggcca
     2581 ggaaggtctg acatgagccg tcttggcacg ctgggccaca agctctacac cggccaggct
     2641 tcgtatgagt tcgtcggtcg ctggcggcgc tggctggcac tgtcgggcgt cgtgattctc
     2701 atcggcatca tcgggctcgc gacacgcggg ctcagcctca gccttgagtt caagggcggc
     2761 acccagttca ccttccctga caaggggtct tcggtggcag cagtgacgaa ggttgtcgtc
     2821 gacgccggtg caccgagccc ggtcgtcgag aaactgtcga acgcgggcct cggcgtcgcg
     2881 aactaccagg tgcagaccaa gccattgacg accgacgaga tcaccaaggt gaccgatgcg
     2941 atcacctcga acctctccgt caccaagcag caagtctcgc tcgacaccgt cggctctagt
     3001 tggggatcgc agatcaccca caaggccgtc gagagcctca tcatcttcct cgtcgcgatc
     3061 gtcatctacc tgtcgctgcg ctacgagtgg aagatggccg ctgcggcgat catcgcgctg
     3121 atccacgacc tgatcatcac gaccggcatt tatgcattgg tcggtttcga cgtgtcgccc
     3181 tcgaccgtca tcggattgct cacgatcctc ggttactccc tgtacgacac cgtggtggtg
     3241 ttcgacaagg tcagggagaa cactgcgccg atcgcgggcg gcagccggta tacctacgcc
     3301 gaagcggcga acctggccgt caaccagacc ttggtgcggt cgatcaacac ctcgatcatc
     3361 gccttgctgc cggtggcatc gctgctgttc gtcggtgccg gactgctcgg cggaggttcg
     3421 ctgaaagacc tcgcgctcgc actgttcgtc ggtctcgcaa ccggtagcta ctcgtccatc
     3481 ttcatcgcga cgccgctgct cgtgctcttc aaggagcgcg agccgcagta catcgcgttg
     3541 acgaagcggg tctcggctcg tgtcggctcc tcgactcgcg cggcgaatgt ggtggcagca
     3601 gcggccgcag gtgcggcggc gaccagcggc ggcgggatct cgaccgcggt cgccgatcgc
     3661 ccggcgccga tcgatgtcga caacacacct gagaccgtcg atccaccggc agccggaacc
     3721 gcggttactc gcgcgccgac gccaccggtt cggcgcccag gcacaccccc tcgacgacag
     3781 ggcgggcggc cctcgggcaa gaagcgccgg cgatgaccgc cggggaggtc gaggagctga
     3841 tccgcgggct ggtccgcgac gtccctgact acccgagccc cggcatcttg ttcaaagaca
     3901 tcacgccgtt gctcgccgat gcagcggcgt tcgcggcgat cgtcgacgcc ctggtcgaac
     3961 agcacgcccc tggcagcatc gacaaggtcg tcggcatcga ggcccgaggg ttcatccttg
     4021 ccgctcccgt cgcctaccac ctgaacgccg gcttcgtccc gatgcgcaag aagggcaagc
     4081 tgccctacca gaccttcgag accagttacg ccctcgagta cgcgagcgcg acgatcgagg
     4141 tgcatcgcga tgcgttcgcg ccgggtgacc gcgtgctcat cgttgacgac gtgctcgcga
     4201 ccggtggcac ggcgctggcc gctctcgacc tcgtgcatcg agcaggcggg acgccggtcg
     4261 ggttctcgtt tctgctcgag ctgagggccc tcgccggtcg ggcggccttg gcgggtgcgg
     4321 gcgagggaca catccggtcg ttgttcatcg tctgaccggg atcccttcga cacgcgtttc
     4381 agcggtttcg ggtcggtaga cttcaagctg aggccaccga aggggtacta gtgccgagca
     4441 gggccgttcc cgaacgcgtc gcgaacgcgc tcgatgagcc tgcgcccgac atgccaggag
     4501 tcaccgaact cgccggagtc gtcgccgtcg cggccgacgc tgagccggtc gaggaagccg
     4561 agctgatcgc cgagcgtgag ctggcggcgg cagcggccga cggttctgag ggtcccgata
     4621 gcgctgaggg ttccgatggt ggcgatggcg gtgatgacgc gaacgagcag tctgcgccgt
     4681 cgtccggacg tcgggtgcga gcgcggctgg cccggttggc cacgccccgc acgtcgacga
     4741 ccacgtcacc ggtgctcgag ccgctgatcc gctcgatccg cctcacccac ccaaaggcag
     4801 atacccgcgc gatccagcgt gcgtacgacg tcgccgagca ccaccaccgc ttgcagttcc
     4861 gcaagagtgg cgacccgtac atcacccatc cactcgctgt cgcgacgatt ctcgccgagc
     4921 tcggcatgac acctccgacc attgtggcgg cgctcttgca cgacacggtc gaagacaccg
     4981 agtacaccct cgagacgctg cgcaccgatt tcggtgacga ggtggccgcg ctcgtcgacg
     5041 gcgtgaccaa gctcgaccgc gtcaagtacg gcgacgccgc gaccgccgag acggtgcgca
     5101 agatggtcgt cgcgatggcg cgcgatcctc gtgtcctggt gctgaagctc gctgaccggc
     5161 tccacaacat gcgcacgttg cggtacttgc cggctgagaa gcaggagcgg aaggcgcgcg
     5221 aaacgctgga gatctacgcg ccgctcgcgc accgcctcgg catgaacacg ctgaagtggg
     5281 agctcg
//
LOCUS       DASWVF010000734         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_351832, whole genome shotgun sequence.
ACCESSION   DASWVF010000734 DASWVF010000000
VERSION     DASWVF010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351832"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGD55_12925"
     CDS             complement(<1..342)
                     /locus_tag="VGD55_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871296.1"
                     /translation="MPTTPEATAKVVTADADGSIAVTLQLPIVLSGLRVGSSGYLQAD
                     GSAADGTTQSDNVLFGVGKASNVCVAASTASATVSLLGMADPAAPASGAIFAVTGPGL
                     PALEGNPAKPGT"
     gene            complement(836..2101)
                     /locus_tag="VGD55_12930"
     CDS             complement(836..2101)
                     /locus_tag="VGD55_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY0871297.1"
                     /translation="MIAEVVAVGTELLLGDIVNGNAAWLGRELADLGIDVPLTTAVGD
                     NIGRIATTVGQACARADVVLVTGGLGPTQDDLTREALAEMLGVPLVRDGELEAALRER
                     YIAAGRGDFPPNNLRMTDRPDGASVLQNPAGTAPGLRMQIDGAIVYALPGVPREMQEI
                     FSAWIRPELAELAGPGQVLLSRRLHTVGVWESQVSQALAALDASLAVVGNPTIAYLAS
                     EGQTRVRITAKATSVEVARDMIARVEADVRAVLGDVIYGTDDDTLESVVHGLLLGAGA
                     TVASAESLTGGLLGAALSATPGASATYRGGAVLYATATKAAVIDVPPDLLDARGPVDP
                     DVAVAMADGVRRKFEATYGLATTGVAGPTEQDGNPVGTVYIALVGPGDAAPIVSKLRL
                     AGRRERVRQGSVLTALDMLRRELAGLPQR"
     gene            complement(2104..2769)
                     /gene="pgsA"
                     /locus_tag="VGD55_12935"
     CDS             complement(2104..2769)
                     /gene="pgsA"
                     /locus_tag="VGD55_12935"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0871298.1"
                     /translation="MTAGRESLEPMVVTSDITIVTTLPAELPQAGVANLANALTVARL
                     VMVPIFTALLLSARNEHTGWRLVATGVFVVASLTDRVDGAIARRRGTVTDFGKIADPI
                     ADKALIGAALISLSSLGDLPWWVTVVVLAREIGVTALRFWVIRRGVLAASRGGKVKTV
                     LQTVAIGLYLLPLTGVMASVRAYLMAVAIVITLVTGADYVARAVRLRRRTAGGTAAPQ
                     RAS"
     gene            complement(2766..4220)
                     /gene="rimO"
                     /locus_tag="VGD55_12940"
     CDS             complement(2766..4220)
                     /gene="rimO"
                     /locus_tag="VGD55_12940"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0871299.1"
                     /translation="MSANRSVHLVTLGCARNDVDSEELAARIAEAGWTLTDSDAADIT
                     LVNTCGFVEAAKKDSVDALLAASDLTPGGKVVAVGCLAERYGGELAEALPEAVVLSFD
                     DYATIGERLDDVLAGRPLVPHVPKDRRTLLPISPAQRPAAVADVGIPGHAGGPRVLRK
                     RLDDSPVAPLKLASGCDRRCSFCAIPSFRGAFVSRPPEEILGEARWLAQHGVREIVLV
                     SENSTSYGKDLGDVRLLERLLPQVAAVEGIARVRLTYLQPAEMRDSLIEVLASTPGVV
                     PYFDLSFQHASATVLRRMRRFGDPEQFLDLLARIRSLAPHAGVRSNVIVGFPGETDDE
                     FAELEDFLLAARLDVIGIFGYSDEDGTSAAELPDKVDPDVVADREAGLGRIVEQLIGE
                     RALERVGEIVEVLVDRVVTVDDEDLTETDAEGRADHQAPEVDGTTVLAGVAIAPGTIV
                     SARVVAAIGADLIAEPLDAELVKHEGHSMSVGSP"
     gene            complement(4251..>4623)
                     /locus_tag="VGD55_12945"
     CDS             complement(4251..>4623)
                     /locus_tag="VGD55_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEY0871300.1"
                     /translation="SPSPIVSTPVVSPPPATPTTQAPQSLPGSAVAEAGVHVVLRITT
                     TKCWVEATGSDGPSDVLYQGVLSPGQTQTFDDPQRVSLRLGNAPATDITVNGVDLGAP
                     ATKNSVTSVSFGPGNPLVAQG"
BASE COUNT          825 a         1606 c         1496 g          696 t
ORIGIN      
        1 ggtccccggt ttcgcggggt tgccctcgag ggccggcaaa cccggcccgg ttacagcgaa
       61 tatcgctccg ctagcgggcg cggctgggtc ggccatgcca agcagcgata cggtcgcgct
      121 cgccgttgag gcagccacac agacgttcga cgccttcccc acgccgaaca agacgttgtc
      181 ggattgcgtg gtcccatcag cagccgaacc gtccgcttgc aagtagccgc ttgaacctac
      241 ccgcaacccc gacagcacga taggcagctg cagagttact gcaatactcc cgtcggcatc
      301 ggcggtgaca accttggcgg tcgcttccgg ggtcgtcggc atgatggtca acctcaccga
      361 cgacccgggg gcgtatccac ccccgctgac gtccacgatt tcgccaccgt ggaacaggtc
      421 ggcgcacgaa gtcgtgtccg actggtgcgg cgtaatcgtc aaatcgccgc cagccacgga
      481 agtcacacct gcggcctccg aacctgacgc agccttcgaa gcatgcgaga tcgaagttgc
      541 gcacgcaccg ccgagccccg ctaggcctac gacggccaga attgaggtga gtgagttgcg
      601 cccgccaaga cgacggcccc gcaaggggag caaagccatc gtcgggcccc gattcagccg
      661 ccgtgtgagc ttggtttgat catcgaccat ttctccagga gacttgacag cccaacacca
      721 acagcacaag caacgcctac gaggctccgc ccagcccaga ctcaaccaaa gcggggggag
      781 tcccatgacc ggcgccgcgc gcaacatgtg tgtcgcttct gaaccaccac cggcttcagc
      841 gttgcggcag gcccgccagc tcgcggcgca gcatgtcgag cgcggtcaag acgctcccct
      901 gacgcacgcg ctcccggcgt ccggccaaac gcagtttcga cacaatcggc gccgcatcgc
      961 ccggcccgac gagcgcgatg tagaccgtac cgaccggatt gccgtcctgc tcggtcggcc
     1021 ccgcaacgcc cgtcgtcgcg aggccgtagg tggcctcgaa cttgcgacgc acgccgtcgg
     1081 ccatcgcaac ggcgacgtcg ggatccaccg gcccgcgtgc atcgagcaga tcaggcggga
     1141 cgtcgatcac cgcggccttc gtcgcggtgg cgtagagaac ggcgccaccg cgataggtcg
     1201 ccgaggctcc tggggtcgcg ctgagcgccg cgcccagcaa cccgcccgtg agcgattcag
     1261 ccgacgcgac ggtagcccca gcgccgagca gcaacccgtg gacgaccgac tcgagcgtgt
     1321 cgtcatcggt gccgtagatg acgtcgccca gcacggctcg cacgtcggcc tcgacacgcg
     1381 cgatcatgtc gcgcgcaacc tcgaccgagg tcgccttcgc ggtgatgcgc acacgcgtct
     1441 gaccctccga tgcgaggtag gcgatggtcg ggttgcctac gaccgcaagc gacgcgtcga
     1501 gtgcggcaag cgcctgcgat acctgcgact cccaaactcc gaccgtatgc aaccggcgcg
     1561 acagcagcac ctgtccgggg cctgcgagct cggcgagttc ggggcggatc cacgcactga
     1621 aaatctcctg catctcgcgc ggcactccgg gcaacgcgta cacgatcgcg ccgtcgatct
     1681 gcatgcgtag gccgggtgcc gtcccggccg gattctgcag caccgaggca ccgtcggggc
     1741 gatcggtcat gcgcaagttg ttcggcggga aatcgccccg gcccgcagcg atatagcgct
     1801 cccgcagcgc cgcctcgagt tcgccatcac gaacaagggg gacgccgagc atctcggcta
     1861 gtgcctcgcg cgtcagatcg tcctgtgtcg ggccaagacc accggtcacg agcacgacgt
     1921 cggcgcgcgc gcacgcctgc ccgactgttg tcgcgatgcg gccgatgttg tcgccgaccg
     1981 ccgtcgtcag cgggacgtcg atgccgaggt cggcaagctc gcgaccaagc cacgcagcgt
     2041 tgccgttcac gatgtcgccg agcaggagtt cggtgccgac ggcgactacc tccgcaatca
     2101 ccgctagctc gcgcgctgcg gcgcggcagt tccacctgcg gtgcggcggc gcaaccgaac
     2161 cgcacgcgcg acgtagtcgg cgccggtgac caacgtgatg acgatcgcaa ccgccatcag
     2221 gtacgcgcgc accgacgcca tcacaccggt gagtggcagc aggtagagcc cgatcgcgac
     2281 ggtctggaga accgtcttca ccttgccgcc gcggcttgcc gcgagaacgc cccgccggat
     2341 cacccagaac cgcagtgcgg tcacgccgat ttcgcgcgcc agcaccacga cagtcaccca
     2401 ccacggcagg tcgcccaagc tcgacaggct gatcagtgcg gcaccgatca gcgccttgtc
     2461 ggcaatcggg tcggcgatct tgccgaagtc ggtcaccgtg ccgcggcgac gtgcgatcgc
     2521 gccatcaaca cgatcggtca acgacgcaac gacaaagaca ccggtggcga ccaagcgcca
     2581 gccggtgtgc tcgttgcgcg cactcagcaa caacgccgtg aagatcggca ccatcacgag
     2641 gcgcgcgacc gtcaacgcgt tcgcgagatt ggcgacgccg gcttgcggca gctcggcggg
     2701 caacgtggtc acgatcgtga tgtcgctggt cacgaccatc ggctcgaggc tctcacgacc
     2761 ggcagtcacg gcgaaccaac cgacatcgaa tggccctcgt gcttgacgag ttcagcgtcg
     2821 agcggttcgg cgatcaggtc ggcgccgatc gccgccacca ctctggccga cacgatggtg
     2881 ccaggtgcga tcgcgacacc cgccaacacc gttgtgccgt caacctcggg cgcctggtgg
     2941 tcggcccgcc cctcggcgtc cgtctcggtc aggtcttcat cgtctacggt cacgacacga
     3001 tcgacgagca cctctacgat ttcgccgacg cgctcgagcg cacgctcgcc gatgagttgc
     3061 tcgacgatgc gtccgagtcc ggcctcgcga tcggcgacca cgtcggggtc gaccttgtcg
     3121 ggcagttctg ctgccgaggt gccgtcttcg tctgagtagc cgaagatgcc gatgacatcg
     3181 aggcgggcgg cgagcaggaa gtcttcgagt tcggcaaatt cgtcgtcggt ctcaccggga
     3241 aatccgacga tgacgttcga ccgcactcct gcatgcggag ccagcgatct gatgcgggca
     3301 agcaaatcga ggaactgctc gggatcaccg aaacgccgca tccgtcgcaa caccgtcgcg
     3361 ctcgcgtgct ggaaagaaag gtcgaaatat gggacgacac cgggagtcga cgccagcacc
     3421 tcgatcagcg agtcacgcat ctccgccggc tgcaagtacg tgagccgcac gcgtgcgatg
     3481 ccctcgacgg cagcgacttg cggaagcagc cgttcgagca gccggacatc gccgagatcc
     3541 ttgccgtacg acgtcgaatt ctcactgacc aaaacgattt cgcggacgcc gtgttgtgcc
     3601 agccatcgtg cctcgccgag tatctcctca gggggacgcg acacgaacgc gccgcgaaac
     3661 gacgggatcg cgcagaacga acatcgccgg tcgcagccgg acgctagctt cagcggcgcg
     3721 accggactgt cgtcgagtcg cttgcgcaac acccgaggcc cgcccgcgtg gcccggaatg
     3781 ccgacgtcgg caacagccgc gggccgttgc gccggactga tcggcagcaa ggtgcgacgg
     3841 tctttcggca cgtggggaac gagcggtcgg cctgcgagga catcgtcgag acgctcgccg
     3901 atcgttgcgt agtcgtcgaa cgacaacacg accgcctcgg gcagcgcttc ggccagctcg
     3961 ccgccatagc gctcagcgag acagccgacg gcgacgacct tgcccccggg ggtcagatcc
     4021 gatgccgcga gcaacgcgtc gacggagtcc ttcttcgcag cctcgacgaa tccgcaggtg
     4081 ttgaccaggg tgatgtcggc tgcgtcggag tcagtgaggg tccagcccgc ctcggcgatc
     4141 cgagcggcga gttcttctga gtcgacgtcg ttgcgtgcgc agcccaaagt caccaggtgc
     4201 acagaacggt tcgcggacac gccccgagcc tacccgggtg ggtgtagtgc tcagccctgc
     4261 gcgacgagcg ggttacctgg cccgaagctc accgacgtga cgctgttctt ggtcgccggt
     4321 gccccgagat cgacgccatt gaccgtgata tcggtcgccg gagcattgcc gaggcgaagc
     4381 gacacccgct gggggtcgtc gaacgtctgg gtctgtccgg ggctgagcac gccctggtac
     4441 agaacgtcgg agggtccgtc agaaccggtg gcctcgaccc agcacttcgt cgtcgtgatg
     4501 cgcaacacga cgtgaacgcc ggcttcggcc accgccgagc ctggcagcga ctgtggcgcc
     4561 tgcgtcgtcg gcgtcgcagg tggtggggag acgaccggag tcgacacgat cgggctcggg
     4621 ctc
//
LOCUS       DASWVF010000735         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_20766, whole genome shotgun sequence.
ACCESSION   DASWVF010000735 DASWVF010000000
VERSION     DASWVF010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20766"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..773
                     /locus_tag="VGD55_12950"
     CDS             174..773
                     /locus_tag="VGD55_12950"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0871301.1"
                     /translation="MSAYLEIWGRRGPQLAPLETDHVTVGRGPENNVSLSWDRTVSAL
                     HAVIERYAAGWSVRDLGSTNGTFVNNSRLAAEHRLRNGDEIRFGSSRSVFRLRNAPAS
                     TATAAAEPPPHVTRREHEVLVALCRPLLSGDIFTEAATVATIAADLVVTEAAVKFHLG
                     NLYDKFDVRDGGDGVRRSRLANEAIRRRALTLSDLRPPT"
     gene            770..>2446
                     /locus_tag="VGD55_12955"
     CDS             770..>2446
                     /locus_tag="VGD55_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0871302.1"
                     /translation="MSEMWRGRYGSVETIAQGGQGRLLRGVDRQHDRPVALKVRNVAS
                     VDERNQVLAEARVLLSLQPHPGLPTVREDFFEGDSYVLVMDWVDGTSLDQVVAARGAP
                     GMPLPSVLRWATQLAAAIDHLHAHRPVIVHGDVKPANAVLTPDDRVVLVDFGIATNGV
                     ATKSGTIGYAAPEVLDGQAPTAAADIYGLAATLLALLTGRPPGDVRPPWEGVDPTTVE
                     VIERALRPGLALEPDRRPATARELVERMRAWAGADAEVAETPPVRYGVSTPDPTVRNQ
                     APTARRWWRRPSFFPAWLRVSFVAVVVAAIVVGFAFGSKPRSPVRRVQGPDRYTTAAQ
                     VALNSFTHADTAILARGDDLADAIAGSYLAGAQGGGPILLTPPSSLPDDVLHALSHLK
                     VKQVFIIGDNTAISGSVDTVLQAHGMRTTRLAGVTRYSTAGVIEQAGGQAAKLTGSGP
                     TALLVSSGDIPDAVAAGPISFHNRFPLLYTAGTEIPPDTVAALREGGITHVVLVGPVS
                     QIGDAIVTQLSALNITAERIAGAGDPTSESIALARFELQALHWPLPQVDLV"
BASE COUNT          405 a          831 c          781 g          429 t
ORIGIN      
        1 ctgggtactt acagcagcac ttcttcggcc gtggctggtt gggcctcgac tcacagtggg
       61 cctacgtcgt ggtgatgctc gcattctgga cggcggttgc tggcgccatg catcgcgccc
      121 gttggtatgt caccctctaa cgcaatggac actgctcagg caggctgtgc accgtgagcg
      181 cgtacctcga gatatgggga cgccgagggc cgcagctcgc gccacttgaa accgaccacg
      241 tcaccgtagg gcgcggtccg gagaacaacg tgtcgctttc gtgggatcga acggtgtcgg
      301 cgctgcacgc cgtcatcgaa cgctacgccg cgggctggtc ggtccgcgac ctcggttcga
      361 ccaatggcac gttcgtcaac aacagtcggc ttgccgcaga acatcggctg cgtaacggag
      421 acgagatccg gttcgggtcc agccgttcgg tctttcggct cagaaatgcg cccgcgtcga
      481 cggcaacggc ggccgctgag ccaccgccgc acgtcacacg acgcgaacac gaggtactcg
      541 tcgcgctctg ccgcccattg ctcagtggcg acatcttcac cgaagcggca accgtcgcca
      601 ccattgcggc ggacctggtg gtcaccgagg ccgccgtcaa attccacctc ggtaacctct
      661 acgacaagtt cgacgtgcgc gacggaggcg atggtgtccg ccgctcacgg ctggcaaacg
      721 aagccatccg ccgccgcgct ctcacgctct ccgacttgcg gccgcccaca tgagcgagat
      781 gtggcgcggg cggtacggat ctgtcgaaac gatcgcgcag ggtggtcagg gccggctgct
      841 acgcggggtt gatcgacagc acgatcgccc tgttgccctc aaggtccgca acgtcgcctc
      901 agtcgacgaa cgcaaccagg tgctggccga ggctcgcgtg cttcttagct tgcagccgca
      961 tcccggtcta ccgacggtgc gggaggactt tttcgaaggc gacagctacg tgctcgtcat
     1021 ggactgggtc gatgggacct cgctcgatca ggtggtggct gctcggggcg caccgggaat
     1081 gccactaccg agcgtcctgc gctgggcaac ccaactcgcg gcagctatcg accatctgca
     1141 cgctcatcgg ccggtgatcg tgcacggtga cgtcaaaccc gccaacgcag tactgactcc
     1201 tgacgaccgc gtggtgctcg tcgacttcgg tatcgccacg aacggcgtcg cgaccaagtc
     1261 gggcaccatc ggctacgcag ctccggaagt cctcgatgga caggcgccga cggcggccgc
     1321 cgatatctat ggtctcgcag ccacgctgct ggcgctcctc accggtcggc cgcccggcga
     1381 cgtccgaccc ccgtgggaag gcgttgatcc gaccaccgtc gaggtcatcg aacgtgccct
     1441 gcgacccggc cttgcgctgg agcctgatcg ccgcccggcg actgcgcgtg aactcgttga
     1501 gcggatgcgt gcgtgggccg gcgctgatgc cgaggtcgcg gaaacgccgc ccgtgcgcta
     1561 tggagttagc acacccgatc cgacggttcg caaccaggca cctaccgccc ggcgctggtg
     1621 gcggcggccg tcgttttttc ccgcgtggtt gcgagtgtct tttgtcgcgg tcgtcgtcgc
     1681 cgcgatcgtc gtaggcttcg cgttcggcag caaaccacgc tcgccagtac gtcgggttca
     1741 aggaccggat cggtacacaa ctgccgcgca ggtggcgttg aactcgttca cgcatgctga
     1801 caccgcaata ctcgcccgcg gcgacgatct cgccgacgcc atcgcgggca gttacctggc
     1861 cggcgcgcaa ggtggcggac cgatcttgct tacgcctccg agcagcctgc cagacgatgt
     1921 cctgcacgcg ctgtcccacc tcaaggtgaa gcaggtcttc atcattggcg acaacacggc
     1981 gatcagtggc agcgtcgaca ccgtgctgca agcccacggg atgcggacga cgcggctagc
     2041 cggcgtcacg cgctactcca ctgctggtgt tatcgaacag gccggcggcc aagccgccaa
     2101 gctcacggga agtgggccga ccgcgttgct cgtcagttcg ggcgacatcc ccgatgcggt
     2161 ggcggcaggt ccgatttcct tccacaaccg atttccgctg ctctacacgg ccggcactga
     2221 gatcccgccg gacaccgtgg cggctttgcg cgagggtggg atcacgcacg tcgtactggt
     2281 cggccccgtg agccagatcg gcgacgcaat cgtcacccaa ctatcagcgc tgaacatcac
     2341 cgccgagcgc atcgcgggcg ccggtgaccc gacgtccgaa tcgattgcac tcgcgcgctt
     2401 cgaactccaa gctctccact ggccgttgcc acaggtcgat ctggtg
//
LOCUS       DASWVF010000736         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_454180, whole genome shotgun sequence.
ACCESSION   DASWVF010000736 DASWVF010000000
VERSION     DASWVF010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454180"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1074)
                     /locus_tag="VGD55_12960"
     CDS             complement(310..1074)
                     /locus_tag="VGD55_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871303.1"
                     /translation="MKNLFAADVRRIQWRPMAFIVALVIIIFTLIIGVIVFEHTAKHP
                     FDTRTGLPNALATATSPLVLVGFVFGASMFGADCTSRAFTTLLTWESRRARLLFSRAV
                     TCGAVLFCASLAALALLVLVLLPTVVVHGTGDRAAYGSLLALATRSALLVAVASALGV
                     SGAAIGRSTTVAVIGAAAYLVMIEQLLVSHAPDVGRWLLINASLSWIAASPNASNGPG
                     GVGQGHTIATAGLMLLVAVLVMHALATFVLDRRDVV"
     gene            complement(1071..2135)
                     /locus_tag="VGD55_12965"
     CDS             complement(1071..2135)
                     /locus_tag="VGD55_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0871304.1"
                     /translation="MIILAVVIVFGGVWWFVSGRPQQRRQRIQTDMTRGVPAPVVRRR
                     VLAVGPPVVGAVVEFVGVTKAYGRRRALDELDLAVPAGGVFGLLGQNGAGKTTALRCV
                     LGLVRPTSGVCRILGADSGRDLHTVIGRVGALIETPGLTPTISGRRNLALLARLDKIG
                     PAGVDRALRTAGLADRADDAVNTYSLGMRQRLGIAAALLRDPEVVVLDEPANGLDPAG
                     IADIRRLLRGLAAEGRTVIVSSHLLDEVEKTCDRVAVIDAGRCVASGPIEHVLATAGV
                     GTLIVGVADLHAGRDALANAGIEAVLADGNLRVSHDATDAARVTEILAAKGLYVNEMR
                     HERPTLEAVFFGLTSGKADR"
     gene            2238..>2555
                     /locus_tag="VGD55_12970"
     CDS             2238..>2555
                     /locus_tag="VGD55_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871305.1"
                     /translation="MLTRMLAVGVDLSAEDLKTWMASVELSSLGATLVSLDAHVSNDQ
                     IVTAAGLADVVGIDCPLGWPVAFIQFVNEHMQGDVEPREGTPIAWRRHLAYRETDRFV
                     IAET"
BASE COUNT          492 a          820 c          851 g          392 t
ORIGIN      
        1 gaggaagccg gtcacaacac cagggcgcac gacgaaagtc agatcatcga cggcggtcct
       61 cgacccgtag cgcttcgtca gccccttcgc ttcgatcatc gggaccaccg cacgccggtc
      121 agcggttgct tcgtcacgag ggtgaccgta actccgagtt gttcagacag cggaactgtc
      181 cacagcgcag tcacaggtgc tcggcaggtg cgcatcgtca taccgccatc atgagcgcac
      241 gcacgctgcg tcacatcagg ccagaccctg acctagccct gatcgcgacc ctgagatttg
      301 tgcgctggtt tagacgacgt cgcgccggtc gaggacgaag gtcgcgagcg cgtgcatgac
      361 aagcacggcg acgagcagca taagtccggc ggtggcgatc gtgtggcctt gacctacgcc
      421 tccgggcccg ttgctcgcat tcgggctggc cgcgatccac gacagagatg cgttgatgag
      481 cagccatctc ccgacgtcgg gcgcgtggct taccaacagc tgctcgatca tcaccaggta
      541 ggcggcagcg ccaatcacag cgaccgttgt gctgcgcccg atggcggcgc ctgacacacc
      601 gagcgcggag gcgactgcta cgagcagtgc tgatcgcgtg gcgagcgcta gcagggatcc
      661 gtaagccgcg cggtcgcccg tcccgtggac aacgactgtg ggcagcagca ccaacaccag
      721 cagggccaac gcggccagcg atgcgcagaa cagcacagcg ccacaggtga ccgctcggga
      781 gaacagcagt cgagcgcggc gcgactccca ggtgagcaag gtggtgaagg cgcggctggt
      841 gcagtcggcg ccgaacatcg aggctccgaa gacgaacccg acgagaacca acggtgaggt
      901 agcggtcgct agcgcgttcg gcagtccggt gcgcgtatcg aacgggtgct tcgcggtgtg
      961 ctcgaagacg atgacgccaa taatgagggt gaaaatgatg atgacgagcg cgacgatgaa
     1021 agccatgggc cgccactgaa tacggcgcac gtcggcggcg aatagatttt tcatcggtcg
     1081 gccttcccag atgtcaagcc gaagaagacg gcttccaacg ttggcctctc gtgacgcatc
     1141 tcgttcacgt acagcccttt ggcagcaagg atttcggtca cgcgtgcggc atcggtggca
     1201 tcgtggctaa cccgcagatt gccgtcagcg aggacagcct cgatcccggc gttggcgagc
     1261 gcgtcgcggc ctgcgtgtag gtccgcgact ccgacgatga gcgtgccgac tccggcagtc
     1321 gcgaggacgt gctcgatcgg accggaggcg acacaccggc cggcatcgat gacagcgacg
     1381 cggtcgcacg tcttctcgac ctcgtcgagc aggtgactgg agacgataac cgtgcgtccc
     1441 tcggcggcca gtccgcgcag caggcgacgg atgtcggcga tgccggctgg gtcgagtccg
     1501 ttggcgggct cgtcgagcac gaccacctcg ggatcccgca gcaacgcagc agcgatgccc
     1561 aaacgctgac gcatgccgag tgagtacgtg ttgacggcat cgtcggcccg atctgccaag
     1621 ccggccgtgc gcagcgcgcg gtcgacgccc gcgggcccga tcttgtcgag gcgagcgagc
     1681 agggcgaggt ttcggcgtcc ggaaatggtc ggcgtgagtc ccggggtttc gatgagtgcg
     1741 ccaacgcgcc caatgaccgt gtgaaggtca cgcccactgt cggcgcccag gatccggcac
     1801 acgccagagg tcggccgaac aagaccgagc acgcagcgca acgccgtcgt cttgccggcg
     1861 ccgttttgac cgagcaaacc gaagaccccg cccgcgggaa ccgcgagatc gagttcgtcc
     1921 aaggcacgtc gccgtccgta ggccttggtc accccgacga actcgacaac cgcgccgacg
     1981 accggtgggc cgacggccag cacgcggcgc cgaacgacag gcgccggaac cccgcgggtc
     2041 atatcagtct ggatgcgctg gcgccgctgc tgaggcctgc cgctcacgaa ccaccacacg
     2101 ccgccgaaca cgatgaccac ggccagaatg atcacgaggg gcaccgaggg cggacagcca
     2161 cgaccatcaa gctaccgaga tccacctcgc gtgcgcaggt catcggccca tctgcgctgt
     2221 ctacgttggt gaatggcatg ctcacgcgca tgttggcagt cggagtcgac ctctccgccg
     2281 aggatctgaa gacgtggatg gcgtccgttg agttgtcgtc tttgggggca accctagtct
     2341 cgttggacgc gcacgtctcg aacgaccaga tcgtcacggc agccggactg gcagatgtgg
     2401 tgggcatcga ctgtccgctc ggatggccgg tcgcattcat tcagttcgtc aacgaacaca
     2461 tgcaagggga cgtcgaaccg cgtgagggga cgcccatcgc ctggcggcgc catctcgcgt
     2521 atcgcgaaac ggataggttc gtcatcgcgg aaacg
//
LOCUS       DASWVF010000737         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_275629, whole genome shotgun sequence.
ACCESSION   DASWVF010000737 DASWVF010000000
VERSION     DASWVF010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275629"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="glgA"
                     /locus_tag="VGD55_12975"
     CDS             <1..1099
                     /gene="glgA"
                     /locus_tag="VGD55_12975"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY0871306.1"
                     /translation="FIEVEVHCFGKPRDEPGVFAHFPNPTLLEVGANPALLTMSTDLS
                     MAAATGDADILHSHTWYANFAGHVAKLLQDAPHVVTTHSLEPLRPWKAEQLGGGYALS
                     SFCERTAIEAADAVVAVSAGMADDVLRCYPLVDPARLHVIHNGIDTEEYAPTTNTEVL
                     AEHGIDFDRPIVIFVGRITRQKGLPYLLRAAHWFDPEAQVVILASSADTPEIADEVES
                     GIMALQEIRDGVFWIRRMVPKNELIQLFSAATVFVCPSIYEPMGIVNLEAMACETAVV
                     ATRTGGIPEVVADGDTGVLVPIDPDPADPYGAPRDPEAFARAFADAVNALVRDPARAA
                     VMGVAGRKRAVEHFSWTAIAERTRNLYAALL"
     gene            1292..1756
                     /locus_tag="VGD55_12980"
     CDS             1292..1756
                     /locus_tag="VGD55_12980"
                     /inference="COORDINATES: protein motif:HMM:NF039693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6221 family protein"
                     /protein_id="HEY0871307.1"
                     /translation="MSIPSRNTIDNLTEWLLLQIAEDEMKARTAGSESWRWRVVETPT
                     QRDALVRPNGTVCADSWGADRQETGFRGAEAKMFIERFNPERVLADCDSKRRMLARLQ
                     NLSRAWWTTTRSADEARGVIREMALTYANRPGYRDEWRPDLGMRSLQAVSSH"
BASE COUNT          309 a          609 c          557 g          311 t
ORIGIN      
        1 attcatcgag gtcgaggtgc actgcttcgg caagccgcgc gacgagcccg gtgttttcgc
       61 gcatttcccc aacccgactc tgctcgaggt cggcgcgaac cctgccctgc tcacgatgtc
      121 gaccgacctg tcgatggccg cagcgacagg cgacgccgac atattgcatt cgcacacctg
      181 gtacgccaac tttgccggcc atgtggccaa gttgctccag gacgccccgc acgtcgtcac
      241 gacccattcc ctcgagccgt tgcggccctg gaaggcggag cagctcggcg gaggctatgc
      301 cctgtcgtca ttctgcgaga ggacagcgat tgaagccgca gacgccgtcg ttgccgtctc
      361 cgcgggcatg gccgacgacg tgctgcgttg ctacccgctg gtcgatccgg cgcggctgca
      421 cgtgattcac aacgggatcg acaccgagga gtacgcaccg accacgaaca ccgaggttct
      481 ggctgaacac ggaatcgatt tcgaccggcc catcgtcatc ttcgtcggtc gtatcacccg
      541 ccagaagggc ctgccctacc tcctgcgagc ggcacactgg ttcgaccccg aggcgcaggt
      601 cgtcattctt gccagctcgg ccgacacgcc ggagatcgcc gacgaggtcg agagcggcat
      661 catggcgctc caggagatcc gcgacggggt tttctggatc cgccgcatgg tgccaaagaa
      721 cgagctgatc cagctgttct ctgccgcgac cgtcttcgtg tgcccgtcga tctatgagcc
      781 gatgggcatc gtcaacctcg aagcgatggc ctgtgagacc gcagtcgtcg ccacccgcac
      841 gggaggcatc ccagaggtcg tcgccgacgg cgacaccggc gtcctggttc cgatcgaccc
      901 cgaccccgcg gacccctatg gggctccgcg cgaccccgaa gcgttcgcgc gtgccttcgc
      961 cgacgccgtc aacgccctgg tgcgtgaccc tgcgcgcgcg gccgtgatgg gtgtcgccgg
     1021 acgcaagcgt gccgtcgagc acttcagttg gaccgcgatc gccgagcgca cccgcaacct
     1081 ctacgcggcc ctcctctgag catttgaggg cgctgccccg ttaggccgat cgggttgttg
     1141 cggtgcaatc gcacgaatgc cggtcaagag tggggtcagc cgtttcgatg atgtgggtga
     1201 aggcaactgg cctaatcagc cgaccgcctc ccgcaattct tgctccgcta gccagttcca
     1261 cgcgagctcc accggaagga ccgcgagtcc catgtcgata ccgtcccgca acacgatcga
     1321 caacctcacc gagtggctct tgctccagat cgccgaggac gagatgaagg cccgcaccgc
     1381 cggcagtgag tcgtggcgct ggcgcgtcgt ggagacccca acacagcgcg atgcgctcgt
     1441 gcggccgaat ggcaccgtat gcgccgactc gtggggcgct gaccgtcagg agacgggatt
     1501 tcgcggcgct gaggccaaga tgttcatcga gcgcttcaat cccgagcgcg tgctagcaga
     1561 ttgcgattcc aagcgccgca tgctggctcg ccttcagaac ctttcacgag catggtggac
     1621 gacgactcgt tccgccgacg aagcgcgcgg agtcatcagg gaaatggcac tcacctacgc
     1681 gaaccggccc gggtaccgcg acgaatggcg gcccgacctc ggtatgcgga gccttcaggc
     1741 cgtttcatcg cactagcagt gcttgagccg tagtcgagct gcattc
//
LOCUS       DASWVF010000738         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_173135, whole genome shotgun sequence.
ACCESSION   DASWVF010000738 DASWVF010000000
VERSION     DASWVF010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173135"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGD55_12985"
     CDS             <1..270
                     /locus_tag="VGD55_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871308.1"
                     /translation="TTARKAGLRCLIAAQRSVMRRSCCLNDGVVPNADATFLSRSRCL
                     RSEYRRLRRIDMQWRNQPRTDRRNGARRPDPPPRQWRPETRPTNW"
     gene            267..701
                     /locus_tag="VGD55_12990"
     CDS             267..701
                     /locus_tag="VGD55_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0871309.1"
                     /translation="MTTARQALADGRYSDAVGAAGRAAKADPMLVDPHLIGGEALVNL
                     GRDADAIRELRQAVYLQPESASALLLLAGALDRQGEPAAAGRAYRSAAATVGELPPEE
                     VSAFFGGRSADELVALCLRLATQAERTAKAAKSVRLRPRDKP"
     gene            698..1108
                     /locus_tag="VGD55_12995"
     CDS             698..1108
                     /locus_tag="VGD55_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0871310.1"
                     /translation="MIGYITFRLGDRQLACRLDEVREVVRLEGLDILPGMTPPISGVL
                     ELRGNPLPVVDLRLGDSPRGDVLVMASGVDALGAIVDQVNAVHDEDEMVSSGERPSGL
                     PSYVIDVVRHTGVSVGRGGPILLVDLQAMLSVAV"
     gene            complement(1130..2227)
                     /gene="disA"
                     /locus_tag="VGD55_13000"
     CDS             complement(1130..2227)
                     /gene="disA"
                     /locus_tag="VGD55_13000"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEY0871311.1"
                     /translation="MPTNDRFDRTVDEKLRATLATIAPGTDLRDALERILRGNTGGLI
                     VLGYDKTVESLCTGGFNLDIETSAPLMREICKMDGAIVLDNTGTRILKANVHLQPDPS
                     IPTSESGTRHRSAERTAKQTGYPVISVSQSMRIIALYVGGRRYVLDDSGAILSRANQA
                     LATLERYKLRLDEVSGTLSALEIEDLVTVRDAAAGAQRLEMVRRIASEIEGYVVELGT
                     DGRLLSLQLDELLAGVEPERELVVRDYLPSTSKKSRTPDDVLRDFDMLSASELLDLST
                     IARVMGYAAGADALDAPVSPRGYRLLAKVPRLPNLVVDRLVEHFGGLQKLLAAGIDDL
                     QAVDGVGENRARSVREGLSRLAESSILERYV"
BASE COUNT          377 a          760 c          803 g          427 t
ORIGIN      
        1 acgacggccc gcaaggccgg gctacgttgc ctgatcgccg cacagaggtc ggtgatgcgc
       61 cgatcctgct gcctgaacga cggggtcgta ccgaacgccg acgcgacctt cctgagccgc
      121 agcaggtgcc tccgcagcga ataccgcagg ctgcgccgga tcgatatgca gtggcgcaat
      181 cagccccgca ccgaccgccg gaacggcgcg cggcgccccg atccgccgcc gcggcaatgg
      241 cgaccggaga cacggcccac gaactggtga ccaccgctcg gcaggcactg gccgacggac
      301 ggtattccga cgccgttggc gctgccggcc gcgccgccaa ggccgatccg atgttggtcg
      361 acccacatct gatcggcggc gaggcgttgg tcaatctggg ccgtgacgcc gacgcgatca
      421 gagagttgcg acaggccgtc tatcttcagc ccgagtcggc gtccgccttg ttgttgctcg
      481 ctggcgcgct cgatcgccaa ggcgagccgg ctgccgcagg ccgcgcgtac cggtccgcgg
      541 ccgcaactgt cggtgagtta ccgccggagg aggtctccgc cttcttcggt ggtcgcagtg
      601 ccgatgaact ggtggcccta tgcctgcgtc tggcgactca ggccgagcgg accgccaagg
      661 ccgcgaagtc ggtgcggctc aggccacggg acaagccatg atcggctaca tcacgttccg
      721 tctcggtgat cggcagcttg cctgcaggct cgacgaggtt cgcgaggtcg tacgtctcga
      781 gggactcgac atcctgcccg gcatgacccc gcccatcagt ggcgtgctgg agttgcgcgg
      841 caatccgctg ccggtagtgg atttgcggct gggcgactct cccaggggcg atgtgctggt
      901 gatggcctcg ggcgtcgacg cgctcggcgc gatcgtggat caggtcaatg ccgttcatga
      961 cgaagacgag atggtgtcgt cgggcgagcg gccgtcgggc ttgccgagct atgtcatcga
     1021 cgtcgtccgc cacacaggtg tgagcgttgg gcgtggcggg ccgattttgc tcgtcgactt
     1081 gcaggcgatg ctgtccgttg cggtctgaac gtcgcctgcc gaaagtcgtt cagacgtagc
     1141 gttcgaggat cgatgactcg gccaatcgcg acagaccctc acgcacgctg cgggcccggt
     1201 tctcgccaac tccatcgact gcctgcagat catcgatgcc ggccgcgagc agcttctgca
     1261 gcccaccgaa gtgttcaacc aagcggtcga cgacgaggtt cggcagccgc ggaaccttag
     1321 cgagcagccg gtagccgcgc gggctcacgg gtgcgtcgag tgcgtcggcg ccggccgcgt
     1381 agcccatcac gcgcgcgatc gtgctgaggt cgagcagctc tgatgccgac agcatgtcga
     1441 agtcgcgcaa cacatcgtcg ggggtacgtg acttcttgct cgtcgagggc aggtagtcgc
     1501 ggaccacgag ttcgcgctcg ggctcgacgc ccgcaagaag ctcgtcgagt tgcagactga
     1561 gcagtcggcc atcggtaccg agctcgacga cgtagccttc aatttcgctg gcgatccgac
     1621 ggaccatctc aagtcgctgt gcacccgcgg ccgcatcgcg gaccgtgaca aggtcttcga
     1681 tttccagcgc tgaaagggtt ccgctcactt cgtcgagccg cagcttgtag cgctcgaggg
     1741 tggccagcgc ctggttcgcc cgcgagagga tcgcaccgga atcgtcgagc acataccgcc
     1801 ggccgccgac gtaaagcgcg atgatgcgca tcgactggct aaccgagatc actgggtaac
     1861 ccgtctgctt cgcggtgcgt tctgcgctgc gatgacgggt acccgactcg ctggtcggga
     1921 tcgacggatc cggctgcagg tgcacgttgg ctttgaggat ccgggtgccc gtgttgtcga
     1981 ggacgatcgc gccgtccatc ttgcagatct cgcgcatcag cggcgctgac gtctcgatat
     2041 cgaggttgaa gccgccggta cacagcgatt cgaccgtctt gtcgtaaccg agaacgatca
     2101 gcccgccggt gttgccgcgc aggatgcgtt cgagtgcatc gcgtaggtcg gtgccaggcg
     2161 caatcgttgc gagcgtcgct cgcaatttct cgtcgacggt ccggtcgaac cgatcgttgg
     2221 ttggcaacgg gctcccctgt actccgtgct tcgaaatgcg gccgttactg tgattctagc
     2281 cggtggtcga gaccgcgtac ccgatcgctt gcgcgaggtc ggaaacctcg atcgcatgga
     2341 cgccgggggg gacctgtcct gagtcgg
//
LOCUS       DASWVF010000739         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_403112, whole genome shotgun sequence.
ACCESSION   DASWVF010000739 DASWVF010000000
VERSION     DASWVF010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403112"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>1704
                     /locus_tag="VGD55_13005"
     CDS             27..>1704
                     /locus_tag="VGD55_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0871312.1"
                     /translation="MPIRDDRGQIVGTFGVSRDITESKRLSAELEHRTADLVRVEAEL
                     RRLLESSPDAMVRYDVDLRHVYLNPAATAFLARPSDEVLGKTDRELGLPIDFLDQWEP
                     ALRHVLATGASAQVEFHVETPTPLFMEARMVAECGEGGRVDGVFVIARDLTDRKRAED
                     ALAAQAVQDPLTGVANRVLLVDRLQQALVRLERHPGRLAVMFLDVDRFKVINDSVGHG
                     AGDALLVEIARRLELTARRSDTVARFGGDEFVVLCEQVAALEDAAVMAGRITRALAEP
                     FAYANQIIHITASIGIAVTDDPLTPPASLIRDADAAMYQAKDRGRGNGSFQFFDAGVR
                     ERAVARMEIDGQLRHAIERAEFRLFYQPLVTLDDERRLLGVEALIRWQHPDRGLLAPA
                     EFMAVAEDTNLIIPIGRWVLDEACRQVAQWNATRGPDDALTVAVNVSARQLSHPDLAA
                     DVAAALTRHALPAHLLTIEVTESALLEDAVTSAAIMDELAALGVQLALDDFGTGYSSL
                     GHLRRFPVNILKIDRSFVEGLDGGGGDVAIVGAVTAMAHAMGMTTVGEGIE"
BASE COUNT          314 a          575 c          528 g          287 t
ORIGIN      
        1 ctgactcgtg ggtcctgacg acaaaaatgc ccatccgtga cgaccgcggg cagattgtcg
       61 gaacgttcgg cgtgagccgc gacatcaccg aatccaagcg attgtcggca gagcttgagc
      121 atcgaaccgc cgatctggtg cgggtggagg cggaacttcg tcggctgttg gaatccagcc
      181 ctgatgcgat ggtgcgctac gacgttgact tgcgccacgt ctacctcaac cccgcggcaa
      241 cagccttcct cgcccggccc tccgatgagg tcctgggcaa aaccgaccgt gagttgggat
      301 tgccaatcga ctttctggac cagtgggagc cggcgctgcg acacgttctg gccacgggcg
      361 ccagcgctca ggtcgagttc cacgtggaga cgccgacgcc tcttttcatg gaagcgcgga
      421 tggtggccga atgcggcgaa ggcggacgcg tcgacggcgt tttcgtgatc gcccgcgatc
      481 tcaccgatcg caagcgggcc gaagatgccc ttgccgctca agccgtacag gatccgctga
      541 ccggtgtcgc gaatcgggtg ctgctcgtcg accggctcca gcaggccctc gtgcgcctgg
      601 agcggcaccc cggcaggctg gcggtgatgt tcctcgacgt cgaccgtttc aaggtcatca
      661 atgacagcgt cgggcacggc gcaggggacg ctctactcgt cgagatcgcc cggcggctgg
      721 aactcacggc tcggcgatcc gacactgtcg ccaggttcgg cggcgatgag ttcgtcgtct
      781 tgtgcgaaca agtcgctgcg cttgaagacg cagcggtaat ggccggccgg atcactcgag
      841 cgctcgccga gcctttcgcc tacgcgaacc agatcatcca catcaccgcc agtatcggca
      901 tcgcggtaac tgacgacccg cttacaccgc ctgcatcgct gatcagggac gccgacgcgg
      961 cgatgtacca ggccaaagac cgcggccgcg gcaacggcag cttccaattc ttcgatgccg
     1021 gagtgcgcga gcgagccgtt gcccgcatgg agatagacgg ccaactgcgc catgccatcg
     1081 aacgcgcaga gttccgcctc ttctaccaac ctctggtcac actcgacgac gaacggcgcc
     1141 tgctcggggt agaggcgctg atccgctggc aacatcccga ccgcggactg ctggcaccgg
     1201 ccgagttcat ggccgtcgcc gaagacacca acctcatcat cccgatcgga cgctgggtgt
     1261 tggacgaggc gtgccgccaa gtcgcccaat ggaacgccac gcgcggcccc gacgacgcac
     1321 tgaccgtcgc tgtgaacgtc tcggcacgcc aactcagcca cccagacctg gccgcggatg
     1381 tggcagcagc gctcacccgc cacgccttac ctgcacatct gctcaccatc gaagtcaccg
     1441 aatccgcatt gttggaagac gcagtcacat ccgcggcgat catggacgaa ctcgcagcac
     1501 ttggcgtcca actcgccttg gacgacttcg gcaccggcta ctcatccttg ggccacctcc
     1561 ggcgattccc cgtgaacatc ttgaaaatcg accggagctt cgtcgagggc ctcgacggcg
     1621 gcggcgggga cgtcgcgatc gtcggcgctg tcactgcgat ggcccacgcg atgggcatga
     1681 ccaccgtcgg cgaaggcatc gaaa
//
LOCUS       DASWVF010000740         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_173181, whole genome shotgun sequence.
ACCESSION   DASWVF010000740 DASWVF010000000
VERSION     DASWVF010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173181"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGD55_13010"
     CDS             complement(<1..507)
                     /locus_tag="VGD55_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871313.1"
                     /translation="MSATAATAAVAPASTTLSVTATSHTVAYGGAVAISGRLVRASDN
                     APLPGGRVYLQQSVNGVWTNAGSVLTDGNGDATAYAVGLGVSRSWTLYWPGNATFGAS
                     RSPAVAVTVAGAPGTSASAGSTQLSIVASAANVAYGGSVAFSGRLTLASNSGPLGGGR
                     VYLQQNVNG"
     gene            647..1597
                     /locus_tag="VGD55_13015"
     CDS             647..1597
                     /locus_tag="VGD55_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0871314.1"
                     /translation="MGSRDRIRPAALASSILALAFMLAACTSSHPSPAYSQPPASSSQ
                     PVPKSAAASSPAVAVSQASAAPVTGNVATPNVASSSTPTPGPATQKPTPTATPPNPSP
                     TKAPSKTASPAPPAPLLVQQLASVGNAQQVVIVTSSGWSTDVATLQTFQKDVTGWHVA
                     FPAMAAHIGRDGFSADKHEGDLKTPVGSYGFGTMFGQRASPGVKFPYRVADSQSVWVD
                     DPSSAFYNTWQENAALSGEHLAAAGFATSYAYAVDIAYNTNPVVPGKGSAIFLHVTTG
                     GGTAGCVSIAQSNLFEILRWLDPAQSPIIVMGPTSVIRQY"
     gene            complement(1608..2522)
                     /locus_tag="VGD55_13020"
     CDS             complement(1608..2522)
                     /locus_tag="VGD55_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0871315.1"
                     /translation="MPEATDRFIEAAGGVLWRPAAGGLGVEIALVHRPKYDDWSIPKG
                     KLLPDEHALVGALREVSEETGFVGRPGRALGETRYVKDGSPKRVRYWAVEAVGGGFSV
                     NAEVDQMMWLPPREAALHLSPDRDRQIAQEFALDVRVTTPCVVVRHGSAGERSSFVGD
                     DRERPLDDLGHRQALALIPVLEALRVERVLAADVLRCLDTVGPYAAQRRLSVQSEPLL
                     SESGHLGAPDAAADRLIALLAADPVPTTVCSQGRVIPPLLQRVCADMKAPVPWEGSVP
                     KGGFVVLNIAKDGDTNTIASVDRYRPIA"
     gene            complement(2533..4113)
                     /locus_tag="VGD55_13025"
     CDS             complement(2533..4113)
                     /locus_tag="VGD55_13025"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY0871316.1"
                     /translation="MARGVREAQDSTSGDARTVRETEAKFRVNPTFELPSILGAVAAV
                     TAVDAPTRISMNAVYYDTQDLRLAREGVTLRRRSGGSDAGWHLKLPVLSHTVPDGTGV
                     RDEIQLPDSPAIPTELSRLITVWARTAPLRPVATLASERTTLILRGADGVGLAELVDD
                     TVSVEGGSHNQARFRELEVEATGGDERIVADVAAVLRDAGAVGGEFVPKVVRALGPQA
                     TTPADPPPPLPVDARDPVSDLVAATLRGYTRLLMSYDVAVRRGLDDAVHQMRVATRRL
                     RSALRSFEPLLDKEWADALATELHWLAGVLGAARDSEVLRTRLLVGARELPTEAKPAA
                     VALKLAEICDKQCRAAFDEVYATLISDRYIALVEQLVDAATYPRTTDAARRPSSVVLP
                     PAIRASWATMRRRATAVTRKGRGTPTPTQYHEVRIAAKHVRYLCDAAAPALGPKARKL
                     GLQVARVQDVLGEQHDAVVAAEFLAGVAALPRIGSLGFGLGLLFAREEAAATAAARSF
                     DVIWPDVIKAKRRRWLTN"
BASE COUNT          727 a         1475 c         1382 g          641 t
ORIGIN      
        1 gccgttgacg ttttgctgaa ggtagacgcg accgccaccg agcggtccgc tgttcgacgc
       61 cagggtcaac cgaccgctaa acgccacgct gccgccatac gccacgttcg ccgcgctggc
      121 gacgatgctg agctgggtcg agcccgccga cgcagaggtc ccgggcgccc cggcgacggt
      181 gacggcaacg gccggtgacc ggctcgcgcc aaaagtcgcg ttgcccggcc agtacaaagt
      241 ccacgaccgt gagacgccga gcccgaccgc gtacgcagtc gcgtcgccgt tcccgtcggt
      301 caacaccgag ccggcgttcg tccagacacc gttcaccgat tgctgaaggt agacacggcc
      361 acccggcaag ggggcgttgt ccgaggcgcg gaccagccga ccggagatgg ccacagctcc
      421 gccataagcg acggtgtgac tggtggcggt cacgctcaaa gtggtggacg ccggagcaac
      481 cgcggcagtc gcggctgtgg ccgacaccaa cgaccctccg aggaggccgg ttgcgaaaag
      541 ggcgatggta acccggcgca cgccggagga cgaaatacgc atatctgcat tatcggtact
      601 ggcccgagat cccttgaggc gcccctcagc cagctagcct ggccagatgg gcagccgcga
      661 tcgcatcagg ccagccgcct tggctagctc gatcctcgcg ctcgcgttca tgctggccgc
      721 ctgcacgagc agccacccgt cgcccgcgta cagccaaccg cctgcgtcga gcagtcagcc
      781 cgtaccgaag tccgctgcgg ctagctcgcc ggcagtcgcg gtgagccagg cgagcgccgc
      841 gccggtcact ggaaacgtgg cgactccgaa cgtcgcgagc tcatcgacac caacccctgg
      901 accggcgaca caaaagccaa caccgacggc gacacccccg aacccgtcac cgacgaaagc
      961 cccttccaag accgcgtcgc ctgcaccgcc ggcgccgctg ctcgtccagc agctcgcgag
     1021 cgtcggtaac gcgcaacagg tcgtgatcgt cacgtcgtcg ggctggtcga ccgatgtcgc
     1081 gacgctgcag acgtttcaga aagatgtcac cggctggcac gtggctttcc ccgcaatggc
     1141 agcccatatc ggccgcgatg gcttctcggc cgacaagcac gagggcgacc tcaagactcc
     1201 ggtcggcagc tacggtttcg gcacgatgtt cggccaacgt gcgagtccgg gcgtcaaatt
     1261 cccttaccgg gtagcagatt cacagtctgt gtgggtcgac gacccgtcgt ccgccttcta
     1321 caacacgtgg caagaaaacg cggcgctcag cggcgagcat ctcgcggcgg cggggtttgc
     1381 gacgtcgtac gcgtacgcgg tcgacatcgc ctacaacacg aacccggtgg tgcccggcaa
     1441 gggcagcgcg atcttcctgc acgtcaccac cggtggtggc acggcgggct gcgtctcgat
     1501 cgcacagtcg aacctgttcg agattctgcg ctggctcgac ccggcccaat cgccaatcat
     1561 cgtgatgggc ccgacatcgg tgatccgcca gtactgagca agaaccctca ggcgatcggg
     1621 cggtaccgat cgaccgacgc gatcgtgttc gtgtcgccgt ccttggcgat gttgagcacc
     1681 acgaagccac ccttcgggac cgaaccttcc cacggcaccg gcgccttcat gtcggcacac
     1741 acccgctgga gcagcggcgg aatcaccctg ccctgactac acaccgtcgt cggtacggga
     1801 tcggcagcca gcaacgcgat cagcctgtct gcggctgcgt cgggcgcgcc caggtggccg
     1861 gattctgaca gtagcggctc gctttgcacc gacaacctgc gctgcgcggc gtaggggccg
     1921 accgtgtcga ggcaccgcaa aacatcggca gcgagcaccc gctcgacgcg cagcgcctcg
     1981 agcaccggga tcagggccag ggcctgccga tgaccgaggt cgtcgagggg tcgctcacga
     2041 tcgtccccga cgaatgacga ccgttcaccg gcgctgccat ggcgtacgac aacacagggc
     2101 gtcgtcaccc ggacgtcgag ggcgaactcc tgcgcgatct gccggtcgcg atcggggctc
     2161 aggtgcagag ccgcctcgcg cggggggagc cacatcatct ggtcgacctc ggcgttgacc
     2221 gagaatccgc caccaacggc ctcgaccgcc cagtaccgca cccgcttggg tgagccgtcc
     2281 ttcacgtagc gggtctcacc gagagcgcgg cccggccgcc cgacgaaccc cgtctcttct
     2341 gacacttctc gcagcgcacc aacaagcgcg tgctcgtcgg gcagcagctt gcctttcgga
     2401 atcgaccagt cgtcgtactt gggccggtgc acgagcgcga tctcgactcc gaggccgcca
     2461 gcggcgggtc gccagagcac gccacccgcg gcctcgatga atcggtcggt cgcctcgggc
     2521 atcaggaggc catcagttgg tgagccaacg acgtcgtttg gccttgatga cgtcgggcca
     2581 gatcacatcg aagcttcttg ctgcagcggt cgcggctgcc tcttcgcgcg cgaacagcag
     2641 gccgagaccg aagccaagcg agccgatgcg gggaagcgcg gcgacgccag ccaggaactc
     2701 ggcggcaacg accgcgtcgt gctgctcgcc gagaacgtct tgcacacgcg ccacctgcag
     2761 accgagcttg cgcgccttcg ggccgagggc cggcgccgcc gcgtcgcaca agtagcgcac
     2821 gtgcttcgcg gcgatccgca cctcgtggta ctgcgtcggg gtcggggtgc cgcgaccctt
     2881 gcgggtcacc gcagtagcgc gtcggcgcat cgtggcccag ctggcccgaa tcgccggggg
     2941 cagcacgacc gacgacggtc gccgtgcggc gtcggtggtg cgcgggtagg ttgcggcatc
     3001 gacgagttgc tcgacgagcg cgatgtaccg gtcgctgatc aaggtcgcgt acacctcgtc
     3061 gaaggccgcc cggcattgct tgtcacagat ctcggccagt ttcaaagcga ctgcggccgg
     3121 cttggcttct gttggcagtt cgcgtgcgcc gacgagcaac cgagtgcgca gcacctcgct
     3181 atcgcgcgcg gcaccgagaa cgccggcgag ccagtgaagt tctgttgcca gcgcgtccgc
     3241 ccactccttg tcgagcaacg gctcgaagct gcgcagtgca ctgcgcaggc gccgagtggc
     3301 gacccgcatc tgatgcactg catcgtcgag accgcgtcgc acggcgacgt cgtagctcat
     3361 caggagtctg gtgtagccgc gaagggtcgc cgccaccaga tcgcttaccg ggtcgcgcgc
     3421 gtcaaccggc aacggcggtg gcgggtcggc aggcgtggtc gcctgggggc ctagagcgcg
     3481 gacaaccttc ggcacgaact caccaccgac ggcaccggcg tcgcgcagca cggccgctac
     3541 atcggcgacg atccgttcgt cgccgccggt cgcctcgact tcgagttcgc gaaagcgggc
     3601 ctggttgtga ctgccgcctt cgaccgacac cgtgtcgtcg acgagttcgg ccaagccgac
     3661 accgtcggct ccacgcaaga tcagcgtggt tcgctcgctt gccagcgtcg cgactgggcg
     3721 cagcggtgcc gtgcgcgccc acactgtgat cagccgcgac aactccgttg ggatcgcagg
     3781 tgaatcaggc aactggatct cgtcacgaac cccggtgccg tcgggcaccg tgtgggagag
     3841 aaccgggagc ttcaggtgcc agccggcatc ggacccgccg gaccgacgtc gaagcgtgac
     3901 gccttcgcgg gccagacgca gatcctgcgt gtcgtagtac acggcattca tcgagatgcg
     3961 ggttggcgca tcgacagcag tcacggccgc aactgcgccg aggatcgagg ggagttcaaa
     4021 cgtcggattg acccggaact tggcctcggt ctccctgacg gtccgggcgt cgccgcttgt
     4081 cgagtcctgc gcctcccgaa cacccctggc cactacacca gcctagcgaa gcgccgcatc
     4141 agccaacgac aggagcatcg tggccttgcg atgcgcggtg actgcgtacc aactcgccct
     4201 gaatgtcgac gcccttgttg gccac
//
LOCUS       DASWVF010000741         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_403154, whole genome shotgun sequence.
ACCESSION   DASWVF010000741 DASWVF010000000
VERSION     DASWVF010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403154"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGD55_13030"
     CDS             complement(<1..301)
                     /locus_tag="VGD55_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0871317.1"
                     /translation="MTGYSAAQIARALGQPPPTDEQAAVIESASTSCLVVAGAGSGKT
                     ETMAGRVVWLVANGHVRPDQVLGLTFTRKAAAELAARVRRRLGQLAQLDMHGLVEA"
     gene            complement(298..>1582)
                     /locus_tag="VGD55_13035"
     CDS             complement(298..>1582)
                     /locus_tag="VGD55_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0871318.1"
                     /translation="DLRGRGSLLGSQQLVDVLAAADDSGAASPLQAVAARLAEERRLF
                     YVAVTRARLHLIATAVRSDDQQPSRFLDELVPRQSEQSDERPLARVPRGLDLPSVVAQ
                     LRAVVTTPGIGPDDDGPAPIRKAAAAAQLARKAAAAAQLARLAAAGVRGADPADWYGL
                     GELTDERPLVDDDAPVRVSPSRLEGFRRCPLRSMLESSGGTTASSASQGLGTLVHALA
                     HQAAQENLDAAQLQALLAKAIERVDLGSGWVAIRQRARATEMVRKLAVWMQTNPRALV
                     AAELAFEVTIGRAVMKGQVDRLERDDKDRLVVVDLKTGKNQPKKDELPSNAQLGAYQL
                     AVEHGAFADVPGGGQATGGAELVQLGTDTQGAITQRQDALADSDDPSWALDMVERSAA
                     GMAAHIFDAIVNDMCRSCPVRTSCPVQDEGRQVTS"
BASE COUNT          235 a          573 c          523 g          251 t
ORIGIN      
        1 cggcctcgac gagcccgtgc atgtcaagct gagcgagttg accgaggcgt cggcgcaccc
       61 gagccgcgag ctctgccgcg gccttgcggg tgaacgtgag ccccagcacc tggtcggggc
      121 gcacatgtcc attcgccacc agccacacca cccggccggc catggtctca gtcttgcccg
      181 agccggcgcc agcaaccacc aggcaggacg tcgaggccga ctcgatgacc gcggcctgct
      241 cgtcggtcgg tggtggctgc ccgagcgcgc gggcgatctg ggcagcgcta tagccggtca
      301 cgatgtcacc tgccgaccct cgtcttgcac cggacacgat gtgcgcaccg gacacgagcg
      361 gcacatgtcg ttgacgatgg cgtcgaaaat atgcgccgcc atgcctgctg ctgagcgttc
      421 gaccatgtcg agtgcccaac tcgggtcatc gctgtcggcc agcgcatctt ggcgttgcgt
      481 gatggcgcct tgcgtgtcgg tgccgagttg caccagctcg gcaccgcccg tcgcctgccc
      541 gccgcccggc acgtcggcga acgcgccgtg ttcgaccgcg agttgatagg cgccgagctg
      601 cgcgttcgac ggcagctcgt ccttcttggg ctggttcttt ccggtcttca gatcaacgac
      661 cacgagccgg tctttgtcgt cgcgctcgag ccggtcgacc tgacccttca tgacggcgcg
      721 gccaatcgtg acttcgaaag ccagctccgc tgcgaccagt gcgcgggggt tcgtctgcat
      781 ccagaccgcg agcttgcgca ccatctcggt tgcccgggca cgctgccgga tcgcgaccca
      841 ccccgacccg agatcgaccc gctcgatcgc cttggcgagc agtgcctgca actgcgcggc
      901 atcgaggttc tcctgcgcag cctgatgcgc cagcgcgtgc accagcgtgc cgagcccttg
      961 cgacgccgaa ctggctgtcg tgccgccgga cgattcgagc atcgatcgca gcgggcaacg
     1021 ccgaaatcct tcaagacggg acggcgagac ccgcaccggc gcgtcgtcat cgaccagcgg
     1081 ccgctcgtcg gtgagctcac cgaggccgta ccaatccgct ggatcggcac cgcgcactcc
     1141 ggccgccgcc aaccgggcca gctgggcggc cgccgccgcc ttccgggcca gctgggcggc
     1201 cgccgccgcc ttccggatcg gcgccggccc gtcgtcgtcc ggcccgatgc ctggagtcgt
     1261 gacgacggcg cgcaactgcg cgaccacaga aggcagatcg agcccgcgcg ggactctggc
     1321 cagtggccgc tcgtcgcttt gctcactctg ccttggcacc agttcgtcga ggaagcgcga
     1381 cggctgctgg tcgtcgctgc gcacagcggt ggcgatgaga tgcagtcgag cccgcgtgac
     1441 cgcgacgtag aacagtcggc gttcttctgc gagccgggct gcgaccgcct gcaacgggct
     1501 cgcagcacca gagtcgtcag cggcggcaag gacgtcgacg agttgctgtg atcccaacag
     1561 cgacccacgc ccacgaaggt ct
//
LOCUS       DASWVF010000742         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_148256, whole genome shotgun sequence.
ACCESSION   DASWVF010000742 DASWVF010000000
VERSION     DASWVF010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148256"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGD55_13040"
     CDS             complement(<1..347)
                     /locus_tag="VGD55_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871319.1"
                     /translation="MRSWPVWGRVCAVLVMAFAFGVCAAWAKGQNIGVDPYVAQLRGA
                     AGNLSTPWLLVPFFAGMQSSRPRRGAVVGLAATLCALAGFYLLSTLVENLGGHGFLGD
                     LRLELSGNRAYFEG"
     gene            complement(357..737)
                     /locus_tag="VGD55_13045"
     CDS             complement(357..737)
                     /locus_tag="VGD55_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871320.1"
                     /translation="MAEVMSTAQRFGHRQHIHLTWLAVTSCGTPRAIVLVSEGIQRTA
                     RYEGAPQKYHATVSRAWVELVGHHVEAHPDADFDGFAEQNAALLDKRLLMRFYSPATL
                     AAEPARSGWVEPDLAPFPWQRSAS"
     gene            962..1255
                     /locus_tag="VGD55_13050"
     CDS             962..1255
                     /locus_tag="VGD55_13050"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEY0871321.1"
                     /translation="MSGIRVTPEQLASLSSRVSSGSASIEGELRSLTGSLAPLGSDWA
                     GVAQQRFQALWAEWQRSAEGLREALGGISQLLGQASSSYAEAERQIAASFGTV"
     gene            complement(1257..>1880)
                     /locus_tag="VGD55_13055"
     CDS             complement(1257..>1880)
                     /locus_tag="VGD55_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEY0871322.1"
                     /translation="LTMTETGSASAPPLPSVPPLLSAPVLVVVQRAKMFSTRAEYDVY
                     APDGTPLGSVHQDPGSGMGIFGQLANVTYEILSADGAVLMRMDKPGAIGRVSFAVSWP
                     SGQPIGTIEQENLLFAPQFELAAADGTTGRLTGGSMLSWDWTIAGPDGAPVGSVSKQF
                     AGLAEMFSSADRFVVELSTQLGGPLRALAVVAAVCLDEVRTQKGRRS"
BASE COUNT          322 a          633 c          608 g          317 t
ORIGIN      
        1 ccttcgaagt aggcgcgatt acccgaaagc tccagcctca ggtcgcccaa gaacccgtgc
       61 ccaccaaggt tttcgaccaa cgtgctcagt aggtagaagc ctgccaatgc gcacaacgtc
      121 gcggccagcc cgacgactgc accgcgtcgg ggtcgcgagc tttgcatgcc ggcgaagaag
      181 gggaccaaca accacggcgt gctgaggttg cccgccgcgc cgcgcagttg cgcgacatag
      241 gggtccaccc caatgttctg gcccttggcc cacgctgcgc ataccccgaa ggcgaacgcc
      301 atgacgagca ccgcgcacac acgaccccac accggccacg acctcaccgc agcaggctac
      361 gacgcggacc gttgccaagg gaacggcgca aggtcaggtt caacccaacc acttcgtgcg
      421 ggttcggcgg ccagcgtcgc cgggctgtag aaacgcatca acagtcgctt atcgaggagg
      481 gcggcgttct gttcggcgaa cccgtcgaag tcggcatcag ggtgggcctc gacgtgatga
      541 ccgacgagtt cgacccaggc gcggctcacc gtcgcgtggt acttctgcgg cgctccctca
      601 tacctcgcgg tgcgctgaat tccttcgctc acaagcacaa ttgctctcgg cgtgccgcaa
      661 cttgtcaccg caagccaggt gaggtgaatg tgttgccgat ggccgaaacg ctgcgctgtg
      721 ctcatgacct ctgccatgag cgtctcgaag gtcggtgggg gccgccggtc gcggcatcgt
      781 cgttcaccat gcttgactcc cctcggttcg ttcttcaaag cgtcgccgtc gggcagctgc
      841 cgtcgcaact cgtttgcgcc gatcgcggcc tgtggacaac gaacgccggc atctcgccgt
      901 ccgacttact gttcgtggcg agtcgcacaa ccgggtgtcg gctcattgtc tggaggtcga
      961 cgtgagcggc atcagggtta caccagaaca gctagcgagt ttgtcgtcgc gggtcagttc
     1021 gggctcggcg agcatcgagg gcgagctgcg gtcgctcacc ggttcgctcg cgccgctcgg
     1081 tagtgattgg gcgggcgttg cgcagcagcg tttccaggcg ctgtgggccg agtggcagag
     1141 gtcagccgag ggtctgcgcg aagcactcgg cggcatcagc caactccttg gccaggcgag
     1201 ttcgagctac gccgaggccg agcgtcagat cgcggcgagc ttcggcacgg tctgagttac
     1261 gaccgacggc ccttttgggt cctgacctcg tcgaggcaca cggccgcaac aaccgcgagc
     1321 gcgcgcaacg ggccgccgag ttgggtgctc agctcgacga cgaatcggtc ggcgctgctg
     1381 aacatctcgg ccaggccggc gaactgcttg ctgacgctgc ccactggcgc gccgtcgggg
     1441 ccagcgatgg tccaatccca gctgagcatg ctgccgcccg tcagccgacc ggtcgtgccg
     1501 tcggcggccg ccagctcgaa ctgcggcgcg aacagcaggt tctcctgctc gatcgtcccg
     1561 atcggctgcc cactgggcca gctcaccgcg aagctgactc gcccgatcgc gcccggcttg
     1621 tccatgcgca tcagcaccgc gccgtccgcg ctaagaattt cgtaagtgac gttcgcgagc
     1681 tgaccgaaga tgcccatgcc cgagcctggg tcttgatgca ccgatcccag cggagtgccg
     1741 tcgggtgcgt agacgtcgta ctcagcccgg gtcgaaaaca tcttcgcccg ctggacgacg
     1801 accagcaccg gcgccgacaa cagcggcgga actgagggca gcggtggagc cgaggcactc
     1861 cccgtttcgg tcatggtcaa
//
LOCUS       DASWVF010000743         4572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_479828, whole genome shotgun sequence.
ACCESSION   DASWVF010000743 DASWVF010000000
VERSION     DASWVF010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479828"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGD55_13060"
     CDS             <1..1062
                     /locus_tag="VGD55_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY0871323.1"
                     /translation="LREGQVFFVHNRVMDIEKRAAEIRGLVPEARVAVAHGQMDEGSL
                     EKVVFDFWEGNYDVLVCTTIVESGLDIPNANTLILERADLLGLSQLHQLRGRVGRGRE
                     RAYAYFLYPVERPMTETAHDRLATIAQNSDLGSGIAIAMKDLEIRGAGNLLGGEQSGH
                     IAAVGFDLYVRLVGEAVADFKGDHTQELAEVRIDLPVDAHIPHDYLEGERLRLAAYRA
                     MAEATSEADVEAVREELADRYGPLPEAVANLLAVADLRVRLRPYGITEVVLQGAQIRF
                     GPVSLRESQVLKLQRLFPGSLVKPLVHTILVPRPADGPARNGGQPLRDVPLLAWVLAL
                     AEAVLEPQSAPRVSADRVE"
     gene            1153..2538
                     /locus_tag="VGD55_13065"
     CDS             1153..2538
                     /locus_tag="VGD55_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0871324.1"
                     /translation="MRRDKRPGVLIVVQNLPVPMDRRVWLECGALTAAGYQVSVICIA
                     GPNDPPYELLEGVHIYKYKPAPQANGTLGFVWEFAYSWSRAAALSVRVWRQHGFDVLQ
                     ACNPPDTYWLLGFLYRLVGKRFVYDQHDLNPEVFLSRFGEPTGVVKRAQYRMLLTLEA
                     LTYRFAHHVISTNESYRDVAISRGHRKASDVTVVRSGPDTSVMRPVAESAPDASPLRN
                     GRRYLVCYLGIMGPQDGVDVLLQAVDYYVHVLGRDDCQFALLGFGDCLPMLREMSTAL
                     ELDDYVTFTGRAEKATIAQYLSAADVGASPDPLNPLNDISTMNKTMEYMAYAVPVVAF
                     DLKETRVSAQEAAVYVVPGDIAGFASALGDLLDDDDRRAEMAVQARLRAAAELDWRPQ
                     AIAYVGVYDQLLGYSGDGRRKSDAWPAVERRSDDVAPHDRDGKPLVDLRGGADLQHFV
                     RNRRIPDEPEA"
     gene            2653..3423
                     /locus_tag="VGD55_13070"
     CDS             2653..3423
                     /locus_tag="VGD55_13070"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0871325.1"
                     /translation="MSRLSSVRAGGLAAVAVLGVALCASACGPLHEGAAATVGGTRIS
                     VAALNAAVTKATATPTADAPADRTGTVQSTLTALIQGDLIADGAKQKGVTVSEADIQT
                     FLEAQRQTNGSDAATAQANGIALSDLHQAVYEALLLNKLIAIVSAGTKDQTAQQKDLT
                     DYLGKVAGSEGVSVNPRYGAWNPSSSTSLPTTPSRPARRRRPYPGPDRSRWRTWRRTP
                     TRCPRVRASGSSSSSRSWTGSGHLGVARGTPSRRMRRW"
     gene            3312..4004
                     /locus_tag="VGD55_13075"
     CDS             3312..4004
                     /locus_tag="VGD55_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEY0871326.1"
                     /translation="MPARKGERFLELVEVMDRLRSPGGCPWDAEQTHASLVKYLLEEA
                     YETAEAIEDADVLALREELGDVLLQVVFHARIAQENAAQAFDIDDVAAGIIDKLVRRH
                     PHVFAGAQVDTAGDVEANWEALKAAEKGRTSPADGVPMAQPALALASALLGRSAKAGR
                     PVEVDTAIEAPVIVDEESIGQLLLAVVALARAAHVDAEAALRAAARRVRADIVELGAD
                     LGSREADVTGLS"
     gene            complement(3997..4302)
                     /locus_tag="VGD55_13080"
     CDS             complement(3997..4302)
                     /locus_tag="VGD55_13080"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0871327.1"
                     /translation="MSDTPSPSGTIRLRTDRISFVEVDGRQLILDSRRSVYYAVNETG
                     LHLWSALRKGTTEAELRRMLIEDHGAGEGEASIDIAKFLKQLDDHGLLTRTPLPLIS"
BASE COUNT          748 a         1483 c         1578 g          763 t
ORIGIN      
        1 ctccgggaag gccaggtctt cttcgtccac aaccgggtga tggacatcga gaagcgggcc
       61 gccgagatac gcggactggt gcccgaggcc cgggtggcgg tcgcccacgg ccagatggac
      121 gagggcagcc tggagaaggt cgtgttcgac ttctgggaag gcaactacga cgtgctggtg
      181 tgcacgacga tcgtcgagtc gggtctcgac atccccaacg ccaacaccct gatcctcgaa
      241 cgcgccgacc tgctcggact ttcgcagctg caccagctgc gcggccgggt gggccgcggc
      301 cgcgagcgtg cgtacgccta cttcctctat ccggtcgagc gaccgatgac cgagaccgcg
      361 cacgatcgcc tggccacgat cgcgcagaac tccgacctgg gatcgggcat cgcgatcgcc
      421 atgaaggatc tcgagatccg cggcgcgggc aacttgttag gcggcgagca gtcgggtcac
      481 atcgccgcag tcggcttcga cctgtacgtg cgcctcgtgg gtgaagccgt cgccgacttc
      541 aagggcgatc acacccaaga actcgccgag gtgcgcatcg atctgcccgt cgacgcgcac
      601 atcccgcacg actacctcga gggggagcgg ctgcgcctgg ctgcgtaccg ggcgatggcc
      661 gaagctacat cagaggcgga cgtcgaggcg gtacgcgaag agttggccga ccgatacggc
      721 ccgttgccgg aggcggtcgc gaacctgctg gcggtggcgg acttgcgggt tcgcctgcgg
      781 ccctatggca tcaccgaggt tgttctccag ggcgcccaga tccgctttgg cccggtgagc
      841 ctgcgcgaat cgcaggtgct gaagttgcag cgccttttcc ccggcagcct cgtcaaaccg
      901 ctggttcaca cgatcctggt tccacggccg gccgatgggc ctgcgcgaaa cggcggacag
      961 ccactgcgcg acgtcccgct gcttgcttgg gtacttgctc ttgccgaagc cgtcctggag
     1021 ccacagtcgg cgccccgagt cagcgccgat cgggtggagt aactcgaact ggtcatgcgc
     1081 cgcgagcgcg aaccttacga tggcatgagg ggcgccgagc agctccttac ggtttggcga
     1141 cgaggaggac gcgtgcggcg cgacaagcgg cctggcgtct tgatcgttgt gcagaacctg
     1201 ccggttccga tggaccgcag ggtgtggctc gaatgtggag ccctgactgc cgctggttat
     1261 caggtgtcgg tgatctgtat cgccggcccg aacgatccgc cgtatgagtt gctcgagggc
     1321 gtgcacatct acaagtacaa gccggcgccg caagccaacg gcactctcgg attcgtgtgg
     1381 gagttcgcgt acagctggtc gcgtgccgcg gcgttgtcag tgcgggtgtg gcggcagcac
     1441 ggattcgatg tgctccaggc ctgcaatccg cccgacacgt actggctact cggcttcctg
     1501 taccgcctcg tgggcaagcg ctttgtctac gaccagcacg atctgaatcc cgaggtgttc
     1561 ctctcccggt tcggcgagcc gacgggcgtc gtcaaacgcg cgcaataccg catgctgctc
     1621 acgctcgagg cgttgaccta ccggttcgcc catcacgtga tctcgacgaa cgagtcctac
     1681 cgcgacgttg cgatctcacg cgggcatcgc aaagccagtg acgtcaccgt cgtgcgcagc
     1741 gggcccgaca cgtccgtgat gcgcccggtg gcagagtcgg cccctgacgc gtcgccactg
     1801 cgcaacggtc gccgttatct cgtgtgttac ctgggcatca tgggtccaca agatggcgtc
     1861 gacgtcttgt tacaggcggt cgactactac gttcacgtgc tcggccgtga cgattgccag
     1921 ttcgcgttgc tcggtttcgg cgactgcctt ccgatgttgc gggaaatgtc gaccgcactc
     1981 gaacttgacg actacgtgac gttcaccggc cgggccgaga aggcgaccat cgcgcagtac
     2041 ctttcagcgg ccgacgtggg cgcctcacct gatccgctga acccgctcaa cgacatctcc
     2101 acgatgaaca agacgatgga gtacatggcc tacgcagttc cggtcgtcgc gttcgatctc
     2161 aaggagacca gggtctccgc acaggaagcg gccgtctacg tggtgcccgg tgacatcgcg
     2221 gggttcgcgt cggcgttggg cgacctgctc gacgacgatg atcggcgcgc cgagatggca
     2281 gttcaggcgc ggttgcgcgc ggcggccgag ctcgactggc ggccgcaagc catcgcgtac
     2341 gtcggtgtct acgaccagtt gctcggttac agcggtgacg ggcgccgcaa gtccgacgcc
     2401 tggccggccg tcgagaggcg cagcgacgac gtggcgccac acgaccgtga cggcaaaccg
     2461 ctcgtcgatc tgcggggcgg tgccgacctc cagcacttcg tccgcaaccg tcgcatcccc
     2521 gacgagcccg aagcctgacc agcggctccc gcggaccctg acttctgctc gacctggcag
     2581 gcccgcgaga atgtgccgac aggcacgatc gatactgggt cgatcctgcg acgccgatca
     2641 gggagggccg acgtgtctcg cctcagttcc gtgcgcgcgg gcggtctggc agcagttgcc
     2701 gtactgggcg tggccctgtg cgcctccgcc tgcgggccgt tgcacgaggg cgcggccgcg
     2761 accgtcggcg gtacccggat ctcggtcgct gctctgaacg cggcagtcac gaaggccacg
     2821 gccaccccga cggccgacgc tccggccgac cgcacgggca cggtgcagtc gaccttgacg
     2881 gcattgatcc agggtgacct gatcgccgac ggggcgaagc aaaaaggtgt gacggtgtcg
     2941 gaggccgaca tccaaacctt cctggaagcg cagcggcaga cgaacggctc tgacgccgcg
     3001 actgcgcagg ccaacggcat tgcgctaagc gacttgcacc aggcggtgta cgaggcactg
     3061 ctgctgaaca agctgattgc gatcgtctcc gcgggaacga aggaccagac cgcgcagcag
     3121 aaggacctga ccgactacct gggcaaggtc gcggggagcg aaggcgtgtc ggtcaatccg
     3181 cggtacggcg cctggaaccc gagtagttcg acgtcgttgc caacgactcc ttctcgtccg
     3241 gctcgtcggc gccgtccgta cccgggacct gaccgcagcc ggtggcggac gtggcggagg
     3301 acaccaaccc gatgcccgcg cgtaagggcg agcggttcct cgagctcgtc gaggtcatgg
     3361 accggctccg gtcacctggg ggttgcccgt gggacgccga gcagacgcat gcgtcgctgg
     3421 tgaagtacct gctcgaagag gcgtacgaga ctgcagaagc gatcgaggac gccgacgtgc
     3481 tcgcgcttcg tgaggagctt ggcgacgtct tgcttcaagt ggtcttccac gcccgcatcg
     3541 cgcaggaaaa tgctgctcag gccttcgaca tcgacgacgt cgcggccggg atcatcgaca
     3601 agctggtgcg caggcatcca catgtcttcg ccggtgcaca agtcgacacg gctggcgacg
     3661 tcgaggccaa ctgggaggcg ttgaaagccg ccgagaaggg gcgcacgtcg cccgctgacg
     3721 gcgtcccgat ggcccaaccc gctctcgcgc tcgcctctgc gctgctcggc cgcagcgcca
     3781 aggcgggccg accggtcgag gtcgataccg ccatcgaggc gccggtgatc gtcgacgagg
     3841 agtcgatcgg ccagctgcta ctcgcggtgg ttgcgttggc gcgggctgcg cacgtcgatg
     3901 ccgaggcggc cttgcgtgca gccgcgcgaa gggtgcgagc cgacatcgtg gaattgggcg
     3961 cggacctggg ctcgcgcgag gctgatgtga cggggctcag ctgatgagcg gcagcggcgt
     4021 gcgggtgagc agcccgtggt cgtcgagctg cttgaggaac ttcgcgatgt cgatgctggc
     4081 ttccccttcg ccggcaccgt gatcttcgat cagcatgcgt cgcagctcgg cttcggtcgt
     4141 gcccttgcgt agcgccgacc acaggtgcag gccggtctcg ttgaccgcgt agtacacgga
     4201 gcgccgggaa tcgagaatga gttgccggcc gtcgacctcg acgaaggaga tgcgatcggt
     4261 gcgcagccga atagttcctg acgggctcgg ggtgtcgctc atcggccgaa ggggtcctct
     4321 cagctccggg ccgtcgaagg cgccgggggc aaccgtagcg cggttgcgcc ggtcgggtga
     4381 agatccgcct gggtgcacac ccttacgggt tgcgccgagg gcgcgccggg cagccgggct
     4441 gtgcgcaggg cggccgcctc gggcggataa actcggtaac gcccgctcaa atccagcccc
     4501 gtgcgcaaaa gcgtcaggag tgccaccgtg gccgccatcg aagccctcgg ggctcgcgag
     4561 atcctcgatt cg
//
LOCUS       DASWVF010000744         7252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_21130, whole genome shotgun sequence.
ACCESSION   DASWVF010000744 DASWVF010000000
VERSION     DASWVF010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21130"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VGD55_13085"
     CDS             <1..727
                     /locus_tag="VGD55_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0871328.1"
                     /translation="LKRASAKRITVVAPFYGYARQDKKHRGREPISARLLADMYKAAG
                     ADRLMVVDLHTAQIQGFFDGPVDHLFALPILAEYLESKLDTSHITVVSPDAGRVRVAE
                     RWTDRLSCGLAIIHKRREPGMAGEVKVHEVVGDVAGRTCILVDDMIDTAGTITKAAEA
                     LMENGASEVVVAATHAVLSGPAVDRLKNSPISEVVVTDTLPVPDDRRFDKLTVLTIAP
                     LLARAISEVFEDGSVTSLFDGAS"
     gene            753..1727
                     /locus_tag="VGD55_13090"
     CDS             753..1727
                     /locus_tag="VGD55_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY0871329.1"
                     /translation="MTSETSDRRKLRAVVTGGAGFLGSHLCERLLDDGYDVVCLDSFL
                     TGTADNVAHLIGNPSFSLVRVDVTEYVHVPGRVDAVLHFASPASPVDYLKLPIETMKV
                     GSLGTLHSLGLARDKGARFLLASTSETYGDPQVHPQPETYWGHVNPVGPRGVYDEAKR
                     FAEALTMAYRRTHGVDTKIVRIFNTHGPRMRPNDGRAIPAFASQALTGKPITVAGDGS
                     QTRSIIYVSDLVEGIVRLLNSDLAGPVNIGNPYEVSVLYLAETVKRLCGSTSEIVFIP
                     RPEDDPMVRQPDITLAREQLGWEPRVEYEEGLERTIGWFRTHADLIGR"
     gene            1853..2509
                     /locus_tag="VGD55_13095"
     CDS             1853..2509
                     /locus_tag="VGD55_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY0871330.1"
                     /translation="MPEVSIAAEPRLEFGKGAARRVRRGHKVPAVLYGHGTPPRHVSL
                     PGHELMRALKSANVLLRLEGLEGGAELALPKAVQRDAVSGAVEHVDLILVRRGEQVTI
                     EVPVIVTGALIPDGFLDQQLVSLSVSAEATHIPQSIEVSIQDLAIGQTVHAGQVKLPE
                     GSTLQTDPDAVVVHITGAQSIAQFESELAEAESELAPAVSEVPAEAVTEEPEAEAPAS
                     "
     gene            2548..3207
                     /gene="pth"
                     /locus_tag="VGD55_13100"
     CDS             2548..3207
                     /gene="pth"
                     /locus_tag="VGD55_13100"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0871331.1"
                     /translation="MAEVAAASRYPWLIVGLGNPGPAYAGNRHNVGFMVVDVLASRAG
                     GRFKGHRSNAEVVEGRLNGTVGAAGTGGSVGERVVLAKPRTFMNESGGPVAGLKAFFG
                     VPLDRLIVVHDELDIDFETLRIKDGGGDNGHNGLRSITKSLGTREYLRVRVGIGRPPG
                     RMDPAAFVLRDFSPTERAALPVNLERAADVVESVLDHGLAATQNRFHSDTQNGYQTDG
                     A"
     gene            3483..5111
                     /locus_tag="VGD55_13105"
     CDS             3483..5111
                     /locus_tag="VGD55_13105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0871332.1"
                     /translation="MTDLIDRGSRVGESSLYKDRMLSVVQSGGSWPQVERRRGPRRHA
                     EVDQTLDRRAPVEPILQLAVLPEIARRGQGADRHLRGYRTSVYAIDVVAAVIGATMAY
                     LVRFGPAAHGSDARYAWGIAVLPLLWLVGVGASRAYEARFLASSTEEYRRVLNAGLGL
                     IAGISVVSYTLKAEFARGYVVTALPVCVAVSMLGRLVARRHLRKARARGLCMQNVLVA
                     GHEWSVLDLVNELRAHPESGLNIVGACLPGGHGSRRMAEAGVPVVGDLNHVVTAVNKL
                     QVDVLAVTTCVEFGGAELRQVCWALENVDVDIVVASALIEVTGPRLHIRPVAGLPLLH
                     VEKPEFSGARRMLKGLFDRLVALTAIVLLSPVLIAIALLVRLTSKGSAFFKQKRVGVR
                     GEVFTMWKFRSMHTDAEARLVAVRDLNENADGLLFKIRDDPRVTRVGKVLRRYSLDEL
                     PQLFNVLLGDMSLVGPRPPLPSEVELYADDVRRRLLVKPGVTGLWQVSGRSDLTWEES
                     VRLDLRYVENWSLFYDFTILWRTAFAVLRGSGAY"
     gene            5310..>7252
                     /locus_tag="VGD55_13110"
     CDS             5310..>7252
                     /locus_tag="VGD55_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HEY0871333.1"
                     /translation="MNRISLRAIVVLASTSALAAGSLQVVASTAAHAASAHTVWVSYP
                     NNDSIEQGDGSLDLVTNFASSVSNVTSSTAPLASSTATNAVNTGLTALLRTSDGSTGA
                     LPPNSSSEPLLGSTRVGLRPTTTVIEGAHSGLTLDLAETKPYVPSSSIDETASQITNS
                     PMMYFTLTVTNSGSSSASPSSVYLGMNGACGAVMNNFPAAGSVAVPLCSSSDSGGTRY
                     LVANTGSGVAAAVGSNLVSGFASSGAVSGSGTSGSAALSLQVPSLGAGSSWSTTLVYG
                     AWNTSAGIKPVSGSTMGFYYRQWFGSAASMLSFAQSEEATALADSSAFDANVASIASD
                     DASQYAASHAFRGWRHEQWLVAAGSTPHYYVTEGAFGYLSTLDVAYEYEPLQTQYEPW
                     KSKLELDEFAGQYETDSSGNRFLQHDLGQWNQLTAGAAYDLKTGTRKHMPVEHNLDFV
                     AMAMLWEDRTGQTYSHSTLQLLISSVEGHDTNSDGLFDVTGMTACRPDGTCRSEPTGT
                     TYDGTVTAAASSEAGNTILGVKAGVVESIAATQGFTAPGGGSLASVAARHLSAEQPFI
                     RDQSSISSLSGWDKRPGYLSDALFYAPMLGSNSTLNASLTWLAQAVHDNHNSVTSACS
                     SCIDRLSSGDALTWISKALDGDVV"
BASE COUNT         1206 a         2346 c         2343 g         1357 t
ORIGIN      
        1 gctgaaacgc gcgtctgcca agcggatcac cgtcgttgcg ccgttctacg gctacgcgcg
       61 gcaggacaag aagcaccgcg gtcgcgagcc gatctccgct cggttgcttg ctgatatgta
      121 caaggcggct ggtgccgatc gcctgatggt ggtcgacctg cacactgcgc agattcaggg
      181 atttttcgac ggcccggtcg accacttgtt tgctttgcca atcctggccg agtacctcga
      241 gagcaagctc gacacgtcgc acatcaccgt cgtttcaccg gacgccgggc gggttcgggt
      301 ggccgaacgc tggaccgatc gtctcagttg tggcctggcg atcatccaca aacgacgcga
      361 gccgggcatg gccggtgagg tcaaggtgca cgaggtcgtc ggtgacgtcg cgggccgcac
      421 ctgcatcctg gtcgacgaca tgatcgacac cgcaggcacg atcacgaagg ctgccgaggc
      481 tttgatggag aacggtgcgt ccgaggtcgt cgtcgctgca acacacgcgg tgttgtcagg
      541 gccggccgtc gaccgtttga agaactcacc gatcagcgag gtcgtcgtca ccgacacact
      601 tccggtgccc gatgacaggc gcttcgacaa gttgacagtg ctcaccattg cgccgctgct
      661 cgcccgcgcg atcagcgagg tgttcgagga cggttcggtg accagcctct tcgatggtgc
      721 gagctgacca ctaacccgcc agaaagggcg acatgaccag cgagaccagc gatcgtcgca
      781 agctgcgtgc cgtggtcacc gggggtgctg gattccttgg ttcccacctg tgcgaacggc
      841 ttctcgacga cggctacgac gtggtgtgcc tcgacagctt cctcaccggt accgccgaca
      901 atgtcgccca tctgatcggc aacccgagtt tctcgctggt tcgggtcgac gtcaccgaat
      961 acgtccacgt gcctggccgc gtcgacgccg tgcttcactt cgcgtcgccc gcgtcgcccg
     1021 tcgactactt gaagctgccc atcgagacga tgaaggtcgg ctcgctcgga acgttgcatt
     1081 cgctcggact cgcccgagac aagggtgcgc ggttcctgct cgcgagcacg agtgagacct
     1141 acggcgatcc ccaagttcac ccgcagcccg agacctattg gggccacgtc aatccggtcg
     1201 gcccgcgcgg cgtgtatgac gaagccaagc ggttcgccga ggcgctgacc atggcctatc
     1261 gccgcacgca tggcgtagac accaaaatcg tgcgcatctt caacacgcat ggcccacgca
     1321 tgcggccgaa cgacggccgc gcgatcccgg cgttcgccag ccaggcgttg accgggaagc
     1381 cgatcaccgt ggcaggcgac ggcagccaga cccgctcgat catctacgtg agcgatctcg
     1441 tcgagggcat cgtgcggctg ctcaactccg acctggccgg gcccgtcaac atcggcaacc
     1501 cctatgaggt ctcggtgctc tatctcgccg agacggtgaa gcggctctgc ggctcgacga
     1561 gtgagatcgt cttcattcca aggcccgaag acgatccgat ggttcgccag cccgacatca
     1621 cgctggctcg cgaacagctg ggctgggagc cgcgggtcga gtacgaggaa ggccttgagc
     1681 gcacaatcgg ctggtttcgc acccacgccg acctgatcgg ccgctgatcg ggtgccggac
     1741 cgctcgaggg gccaggcggg ttcgctgagc ggtgtgccca gcggctagac tgccgtttcc
     1801 gattgtttgc gttgtagtgc cgcccgtttc ttgaaggaag agagtccctg ccgtgcccga
     1861 ggtcagtatc gccgccgaac cgcgcctgga gttcggcaaa ggtgccgctc gtcgcgtgcg
     1921 ccgcgggcac aaggtccccg ccgtcctgta cggccatggc acgccgccgc gccacgtcag
     1981 ccttccgggt catgaactca tgcgggcgct gaagagcgcg aacgtcctgc tccggctcga
     2041 gggccttgaa ggtggcgccg aactcgcgct gccgaaggcc gtccagcgcg acgccgtgag
     2101 cggcgcggtc gagcacgtcg acctgatcct ggtgcgccgc ggtgagcagg tcacgatcga
     2161 agttcccgtc atcgtgaccg gcgcgctgat tcccgacgga ttcctcgacc agcagcttgt
     2221 gtcactgtcg gtctccgccg aggcgacgca tatcccgcag agcatcgagg tctcgatcca
     2281 agatcttgcg atcggccaga cggtgcatgc cggtcaggtg aaactgcccg agggctcgac
     2341 gcttcagacc gatcccgacg ccgtcgtcgt gcacatcacc ggtgcccaga gcatcgccca
     2401 gttcgagtcc gagcttgccg aggccgagtc cgagcttgcc ccggctgtgt ctgaggtgcc
     2461 tgcagaagcg gtcaccgagg agcccgaagc cgaggctccc gcctcctgat cgcgatccac
     2521 agctgcgggt aaggcggggt gcgccgggtg gccgaagtag cggctgcatc gcgatatccc
     2581 tggctcatcg tcggccttgg caaccctggg ccggcgtatg caggcaatcg gcacaacgtg
     2641 ggcttcatgg tcgtcgacgt gctggcatcg cgggcgggtg gccggttcaa gggccatcgc
     2701 tcgaacgctg aggtggtcga gggacgcctg aacggcacgg tcggcgcagc cggcaccggt
     2761 gggtccgtcg gcgagcgcgt ggttctggcc aagccgcgta cgttcatgaa cgagtcaggg
     2821 gggccggtcg ccggcttgaa ggcgttcttc ggcgtccctc ttgaccggct gatcgtcgtc
     2881 catgacgagc ttgacatcga cttcgagaca ctgcgcataa aggacggcgg cggcgacaac
     2941 ggtcacaacg gcttgcgctc gatcacgaag tcgctgggca ctcgcgaata cctgagggtg
     3001 cgagtcggta tcggccgccc gcccggtcgg atggatcctg cggcgttcgt gctcagggac
     3061 ttctcgccaa ccgaacgcgc ggcacttccg gtgaacctcg aacgggcggc tgacgttgtc
     3121 gagagcgtgc ttgatcatgg tctcgcggcg actcaaaacc ggttccacag tgacactcaa
     3181 aacggatacc aaaccgacgg cgcgtgacgc ggactcggat gagttcacat ggcgtttacg
     3241 cggcggtcag attggaacgc tcccggatgt atcagagtcg gccgtttggg gtcacttgat
     3301 gggctgaacg tatgaaatct cgaatgggaa cgtgcctcat caagtgcact gcggcagact
     3361 tgcctggcac agcatgagca cggcatttgg ccttggggac agggccgttt ggcttttgga
     3421 agcttgggac ctttcgggag tcaaggcggc gaggcacagg caagcacggc aggggggttg
     3481 gcatgacgga cttgatcgat cgaggctcgc gagttgggga gtcaagcctg tacaaggacc
     3541 ggatgctgag cgtcgtccag tcgggcggct cctggccgca ggtagaacgc cgtcgcggcc
     3601 cacgcaggca tgccgaggtc gaccaaaccc ttgaccgccg tgcgccggtc gaacccatct
     3661 tgcaacttgc ggtcctgccc gagatcgccc ggcgcggtca gggtgccgac cggcatctgc
     3721 gtggctaccg cacctcggtc tacgcgatcg acgtcgtagc tgccgtgatc ggcgcgacga
     3781 tggcctacct cgtccgcttc ggcccggctg ctcatggatc cgacgcgcgc tacgcctggg
     3841 gcatcgcggt ccttcctctt ctctggcttg tcggcgtcgg tgcgagtcgc gcctacgaag
     3901 cgcgcttcct tgcttcaagc accgaggaat accggcgggt attgaacgcg gggctcggtt
     3961 tgatcgccgg catctcggtc gtctcctaca ccttgaaggc cgagttcgcg cggggctacg
     4021 tcgtcaccgc tttgcccgtc tgtgtagcgg tttctatgct cggccgcctc gtcgctcgcc
     4081 ggcatttgcg caaggcgcgg gctcgaggcc tgtgcatgca gaacgttctc gttgccggcc
     4141 acgagtggtc ggtgctcgat ctcgtcaacg agctgcgcgc gcaccccgag agcgggctca
     4201 acatcgtggg cgcgtgtttg cccggcggtc acggctcgcg ccgcatggca gaggccggcg
     4261 ttcccgttgt cggcgacctc aaccacgtcg tcacggcagt caacaagctc caggtcgacg
     4321 tgctcgctgt caccacctgc gtcgagttcg gtggtgccga gcttcgtcag gtttgctggg
     4381 cacttgagaa cgtcgacgtc gacatcgtgg ttgcttccgc cttgattgaa gtcaccgggc
     4441 cgcgcttgca cattcggccg gtcgccggat tgccgttgct ccacgtcgaa aagccagagt
     4501 tctccggcgc gcgtcgcatg ctcaagggcc tcttcgacag gctcgtcgcg ctgaccgcca
     4561 tcgtgctgct ctcgccggtg ctcatcgcga tcgcgctgct cgttcgactc acctcgaagg
     4621 gctccgcgtt cttcaagcag aagcgagtcg gggtcagagg cgaagtcttc acgatgtgga
     4681 aattccgctc gatgcacact gacgccgagg cgcgcctcgt cgccgttcgc gacctcaacg
     4741 agaacgcaga cggcctgctc ttcaagatcc gcgacgaccc acgtgtcacg cgcgtcggca
     4801 aggtcttgcg tcgctactca ctcgacgagt tgccgcagct tttcaacgtg ctcctcggcg
     4861 acatgtcgct ggtaggcccg cggccgccgt tgccgagcga agtagagctt tacgcggacg
     4921 acgttcggcg gcggttgctt gtgaagccgg gcgtcaccgg gctttggcaa gtgagtgggc
     4981 gctctgacct cacgtgggaa gagtccgtgc ggctcgacct gcgttacgta gagaactggt
     5041 cgttgttcta cgacttcacg atcttgtggc ggaccgcatt cgccgttctg aggggatctg
     5101 gtgcctactg atcggtggtg tccgattcac cccggccact cctccgctct gagcaccgag
     5161 gaatcctcgc aaaagccact ctccgtggcc gcgcgctcac agtcagcagt tccaaaagag
     5221 ttgggagcgc ttcgctccga gccagtagat aatggcggct gtcgtgtcgg cgcaagaccg
     5281 cgtcgacaga ccaccgtggg ggttggggca tgaaccgcat ttcgttgcgc gccattgttg
     5341 tgttggcttc gacgagcgcg ctcgccgcgg gctcgttgca ggtggtcgca tcgaccgcgg
     5401 ctcatgctgc ttcggcgcac acggtctggg tttcctatcc caacaacgat tcgatcgagc
     5461 aaggtgacgg ctccctcgac ctcgtgacga acttcgcgtc gtcggtgtcg aacgtcacgt
     5521 cgtcaaccgc tccgcttgca tcgtcgacgg cgaccaacgc cgtgaacacc ggtttgaccg
     5581 cgctgttgcg tacaagtgac ggctcgaccg gagcgctgcc ccccaactcc tcgtccgagc
     5641 cgttgctcgg ttccacccgc gtcggactgc gaccgaccac gaccgtcatc gagggcgcgc
     5701 attcagggct cacgctggat ctcgccgaaa cgaagcccta cgtgccttcc agttcgatcg
     5761 acgaaacggc ttcgcagatc acgaacagcc cgatgatgta tttcacgctg accgtgacca
     5821 actccggctc gtcgagtgca tcgccgtcgt cggtctacct cggcatgaac ggcgcgtgcg
     5881 gcgctgtgat gaacaacttc ccagctgccg gcagcgttgc ggttccgctg tgttccagct
     5941 ctgactcagg tggcactcgc tacctcgtcg cgaacaccgg ttccggcgtt gcggccgctg
     6001 tcggcagcaa cctcgtgtcg ggcttcgcgt cgtctggcgc ggtaagtggc agcggcacct
     6061 cgggctccgc ggcactgagc cttcaggtgc catcgctggg cgctggttcg tcgtggtcga
     6121 cgacgctcgt gtacggcgcg tggaacacct ccgccggcat caagcccgtc agcggttcga
     6181 cgatgggctt ctactaccgg cagtggttcg gcagcgccgc ttcgatgttg tcattcgccc
     6241 agtcggaaga ggcaactgcg ctcgctgact cgagcgcgtt cgacgcgaac gtcgcgagca
     6301 tcgcatcaga cgacgcatcg cagtacgccg catcgcacgc gttccgcggc tggcgccacg
     6361 agcagtggct ggtcgctgcg ggcagtacac cgcattacta cgtgaccgaa ggtgcgttcg
     6421 gctacctgtc aacgctcgac gtcgcgtacg agtacgagcc gcttcagact caatacgagc
     6481 cgtggaagtc gaagctcgag ctcgatgagt tcgcgggtca gtacgagacc gactcgtccg
     6541 gcaacagatt cttgcaacat gacctcgggc aatggaacca gctcactgcc ggggcggcgt
     6601 atgacctgaa gaccggtact cgcaagcaca tgccggtcga gcacaacctt gacttcgtcg
     6661 ccatggcgat gctctgggaa gatcgcacgg gtcagaccta cagccactcg acgttgcaac
     6721 tgctgatcag ctcggtcgag ggtcacgaca ccaacagcga cgggctcttc gatgtcacgg
     6781 gcatgacggc gtgccgtccc gatggcacct gtcgcagcga gccgaccggc acgacctacg
     6841 acggcaccgt cacggctgcc gcttcgagcg aagcaggcaa cacgatcctc ggcgtcaagg
     6901 ctggcgtcgt cgagtcgatc gcggcaactc aaggcttcac cgcccccggt ggtggcagcc
     6961 tcgcgagtgt ggcggcacgg cacctgtcgg cagagcagcc gttcatccgc gaccagagtt
     7021 cgatcagctc gctcagtggc tgggataagc gtcccgggta cctgtccgac gcgttgttct
     7081 acgcaccgat gctcggtagc aactcgacgc tcaacgccag cctgacctgg ctcgcgcaag
     7141 ccgttcacga caaccacaac tcggtgacgt cggcgtgttc gtcgtgcatc gaccggttgt
     7201 cgagcggcga cgccttgacc tggatttcaa aggcgctcga cggcgacgtg gt
//
LOCUS       DASWVF010000745         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_428631, whole genome shotgun sequence.
ACCESSION   DASWVF010000745 DASWVF010000000
VERSION     DASWVF010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428631"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGD55_13115"
     CDS             complement(<1..224)
                     /locus_tag="VGD55_13115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0871334.1"
                     /translation="MTQRRVCSVLFCDLVGFTPLSESRDPEEVRELLSGYFETARTVI
                     GRYGGVVEKFIGDAVMAVWGAPVAAETDAER"
     gene            complement(504..782)
                     /locus_tag="VGD55_13120"
     CDS             complement(504..782)
                     /locus_tag="VGD55_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0871335.1"
                     /translation="MIARMWETKISPGQLDEFCDWVKATAWPQFVAAPGFSGGELYRS
                     DDHDRIVLVTRWATQEALAGGNEWFDLGAERFLAREPQAWDFSPVELD"
     gene            complement(779..1807)
                     /gene="tsaD"
                     /locus_tag="VGD55_13125"
     CDS             complement(779..1807)
                     /gene="tsaD"
                     /locus_tag="VGD55_13125"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0871336.1"
                     /translation="MSDALVLGIETSCDETGVGIVRGTTLLANAIASSVDEHARFGGV
                     VPEVASRAHLEAMVPTVRRALSEAGVSMRDVDAIAVTAGPGLAGALLVGVAAAKAYSL
                     AFEKPLYGVNHLCAHVAVDVLEHGPLPEPCVALLVSGGHSSLVLVPDLTGELQSLGST
                     VDDAAGEAFDKVARVLGLPFPGGPHIDRVARDGDADLVAFPRGKSDDGTFDFSFSGLK
                     TAVVRWVEARERAGEPVPLAHVAASFQEAVVDVLTMKAIAACRANDVDHLVIGGGVAA
                     NSRLRALAQLRCDSAAVTLRVPRPGLCTDNGAMVAALGARLVEAGLAPSALDLPADSS
                     LPITEVMV"
     gene            complement(1800..2279)
                     /gene="rimI"
                     /locus_tag="VGD55_13130"
     CDS             complement(1800..2279)
                     /gene="rimI"
                     /locus_tag="VGD55_13130"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY0871337.1"
                     /translation="MSLQPMRWWDVDGVMELERDLFGDECWTIAMFWSELAEPETRFY
                     LVARDGTEVVGYAGLCAYADESFVQTIGVKRDRQNQGIGTVLLTALIDEARKRDEPMI
                     GLEVRSDNLVAQRMYERFGFEQVGIRKGYYQPSDTDAVLMVLNLAGVDVTKWAPDHE"
     gene            complement(2321..2971)
                     /gene="tsaB"
                     /locus_tag="VGD55_13135"
     CDS             complement(2321..2971)
                     /gene="tsaB"
                     /locus_tag="VGD55_13135"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0871338.1"
                     /translation="MLLLAFDTSTPAITVALHDGNVVLAERTVVDARSHGEFLAPGIA
                     HVLAVAGASASDLTLVAVGVGPGPFTGLRVGLMTARALGDALSVPVVGVCTLDIVAAT
                     VASGEPFVIATDARRREVYWATYDAHARRIDGPHVDRPADVEWRGPAYGAGALMYKDV
                     FADARLPETPGAELPSAAVLASMVANGTAIVLPPDPLYLRRPDAVEPPPRKVVTPV"
     gene            complement(2998..3480)
                     /gene="tsaE"
                     /locus_tag="VGD55_13140"
     CDS             complement(2998..3480)
                     /gene="tsaE"
                     /locus_tag="VGD55_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0871339.1"
                     /translation="MTVDLNVATADAMHRVGVNLAALLRAGDLVVLTGPLGAGKTTLA
                     QGIGAGLGVRGNVTSPTFVIARVHPPKTAGPPLVHVDAYRIAGALEIDDLDLDASVDE
                     SVTVVEWGRGLVEGLAPDRLDVDIDWAAEAVGETRRVRITAHGPRWHDVELADLLATG
                     "
     gene            complement(3477..4112)
                     /locus_tag="VGD55_13145"
     CDS             complement(3477..4112)
                     /locus_tag="VGD55_13145"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0871340.1"
                     /translation="MSEAEWAKLATQIRACVACTELAATRTNVVVGTAPPSGAAVLLV
                     GEAPGATEDASGLPFVGKAGALLDQLLGEVGLPRPSVAVVNVLKCRPPGNRQPAKAEV
                     LNCRGWLTAQIAVADPQVICALGGTAARWFFGERAKIADLRGRAHDVDGRRVVPTYHP
                     SAAIRFGPAGMPRAALREDLAFVARLVDRSVAAVERPVAAVDRPAGVTPKS"
     gene            complement(4125..5351)
                     /gene="alr"
                     /locus_tag="VGD55_13150"
     CDS             complement(4125..5351)
                     /gene="alr"
                     /locus_tag="VGD55_13150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0871341.1"
                     /translation="MTAITSRRRTHWQDVAVTFSDSSLASGALRAAARIDLGVISANV
                     ARIADVAGPADVMAVVKAEGYGHGLVQTAQAARAGGATWLGVAFLEEALALRAGGIDG
                     RILCWLATPGEPLAEAVLADVDLSASAPWMMGEIAAAAASVDRVARVHLKVDTGLSRG
                     GAVADDWPALVDAALRAQADGVVNIVGLWSHLVHGSEPRHPTTGLQVTAFSDAVDYAT
                     RVGVSPEVRHLANSGGLLFVPDTRFDLVRAGLAIYGLSPAPDEGDAAALGLRPAMTLT
                     ARLALTKKVPADTGVSYSHRYITSAATTLGLVPLGYADGIGRSAANKAEVLVRGRSGD
                     VRRTIAGTVCMDQFVLDLGGADTDEFAAGDEVVLFGPGDRGEPTAQDWADALDTISYE
                     IVTRIGVRVPRVYEGV"
     gene            5341..5994
                     /locus_tag="VGD55_13155"
     CDS             5341..5994
                     /locus_tag="VGD55_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0871342.1"
                     /translation="MAVMQLTKYTHSCVRLDDEDRALVIDPGMFSETGPALDGAHAVL
                     ITHEHADHIDAPALIQAAKADDALRIWAPAAVAHSLVELGDQVTAVDAGESFDAAGFA
                     ITTFGGLHALIHPLVAAPVANVCYLVNGTVFHPGDSFTVPPVPVETLLIPINAPWSKI
                     GEVIDFAAAVRAPRAFQIHDALLNDRGSGLVDSLVGRVSALYGTTYERLAATQTVDV"
BASE COUNT         1099 a         2138 c         1927 g          889 t
ORIGIN      
        1 cgctcggcat cggtctcagc agcgacgggt gcgccccaca ccgccatcac cgcgtcgccg
       61 atgaacttct cgacgacgcc gccatagcgc ccgatcacgg tgcgcgcggt ctcgaagtaa
      121 cccgacaaca actcccgaac ctcttcgggg tcgcgcgact ccgacagcgg ggtgaaaccg
      181 accaggtcgc agaagagcac cgagcacacc cggcgctgcg tcacgcggcc cgcgtcaggc
      241 cgcgaaagct cgctcgtgcc gccaactgtg gcggccacac cacccgtgag agcgaacccg
      301 cactcgccac agaagcgttg accggcagcc gccggcgtcc cacaggatgg gcatgcggtc
      361 gacaacgacg acccgcagga ggaacagaac cggtaccccg gcggattcgt cgcgccacaa
      421 tccgcgcacg tcagcaccag gaaagtagac cgtccgggcg gcggcgctgt ctatggcacg
      481 gcaggacggt ctcgtcgacc ggtctagtcg agctcgaccg ggctgaagtc ccacgcttgc
      541 ggctcgcgtg caaggaacct ttcggccccg agatcgaacc attcgttccc accggccagc
      601 gcctcctggg tggcccagcg ggtgaccaac acgatgcggt cgtggtcatc ggatcggtag
      661 agctcgccac cgctgaatcc cggtgctgca acgaactgcg gccacgccgt ggccttgacc
      721 cagtcgcaga actcgtcgag ttgccctggt gagattttcg tctcccacat gcgtgcgatc
      781 acaccatcac ctcggtaatc ggcagcgacg agtctgctgg tagatccagt gcggacggcg
      841 ccagcccggc ctctacgagg cgtgcaccca gtgccgccac catggcgccg ttgtcggtgc
      901 acaatccggg tcggggcacc cgcagcgtga cagcggccga gtcgcaccgc agctgcgcga
      961 gcgcccgaag acgtgagttg gcggcaacac ccccgccgat caccaggtgg tcgacgtcgt
     1021 tcgcgcggca tgcggcgatg gccttcatag tcaggacatc gaccacggct tcttggaacg
     1081 aagccgccac atgggctagc ggcaccggtt cacccgctcg ctcgcgagcc tcgacccagc
     1141 gcacgacagc ggtcttgaga ccggaaaacg agaaatcgaa cgttccgtca tcggatttac
     1201 cgcgggggaa ggctacgagg tcagcgtcgc cgtcgcgcgc gacccgatcg atgtgcggac
     1261 caccggggaa cggcaaaccg aggacgcggg ccactttgtc gaacgcctcg cccgcggcgt
     1321 cgtcgactgt cgagccgagc gactgcaact cacctgtgag gtctggcacc aacaccaacg
     1381 acgagtggcc gcccgacacc agcagcgcga cgcaaggttc tggcagtggt ccgtgttcga
     1441 gcacatcgac cgcgacgtgc gcacacaggt ggttcacgcc atacagcggt ttttcgaaag
     1501 caagcgagta cgccttcgct gccgccacac cgacaagcag cgccccggca agcccgggcc
     1561 ctgcagtgac ggcgattgcg tcaacgtcgc gcatcgacac accggcttcg gagagtgcac
     1621 gccgaacagt tggcaccatc gcctcgagat gggcacggct cgcaacctca ggcaccacgc
     1681 cgccgaagcg tgcgtgctca tcgacgctcg atgcgatcgc attggcgagc agcgtggtgc
     1741 cgcgcacgat gccaacaccc gtttcgtcgc acgacgtctc gatgccgaga accagcgcat
     1801 cactcatggt cgggcgccca tttcgtgacg tcgactcccg cgaggttcaa gaccatcaac
     1861 acggcatcgg tatcgctggg ctggtagtag cccttgcgga tgccaacttg ttcgaagccg
     1921 aagcgctcat acattcgctg cgcgacgagg ttgtccgacc gcacctcaag cccgatcatc
     1981 ggctcgtctc gtttgcgcgc ttcgtcgatc agcgcggtca acaagacagt gccgatgccc
     2041 tgattctgcc gatcccgttt caccccaatg gtctgcacga atgactcgtc ggcgtatgcg
     2101 cacaggcctg cgtagccgac gacctcggta ccgtcgcgcg cgaccaggta gaacctcgtc
     2161 tcgggttcgg cgagttcaga ccagaacatc gcgatggtcc agcattcgtc accgaacagg
     2221 tcacgttcga gttccatcac gccgtcgacg tcccaccaac gcatcggctg caaactcacg
     2281 cccgcagctt tgctcgatcg ccgcgtggtc ttactcgagc tcataccggc gtcaccacct
     2341 tgcgcggggg cggttcgaca gcatcgggtc gacgcaagta gagcgggtca ggtggcagca
     2401 cgatcgctgt accgttcgcg accatcgacg ccagcacggc ggcgcttggc agttcggcgc
     2461 ccggcgtttc tggcaggcgc gcgtcggcga atacatcctt gtacatcaat gcacccgcgc
     2521 cgtacgcagg cccgcgccac tcgacgtccg ccggtctatc gacatgcgga ccgtcgatgc
     2581 gccgagcatg cgcatcgtag gtcgcccaat agacctcgcg acggcgcgcg tcggtcgcga
     2641 taacgaaagg ctcgccgctg gcgactgtcg ccgccacgat gtcgagcgtg cataccccga
     2701 cgaccggtac cgacagcgcg tcaccgagcg cgcgcgccgt catgagaccg acccgtagtc
     2761 cggtgaacgg tcctggcccg acgccgacgg cgacgagagt cagatcggac gccgatgcgc
     2821 cggcgaccgc gagaacgtgt gcgatccccg gggccaggaa ctccccgtgg ctgcgggcgt
     2881 cgacgacagt gcgctcggcg agcaccacgt tgccgtcgtg caaggcaacc gtgatcgcag
     2941 gagtcgacgt gtcgaacgcg agaagcagca cacgcaagag cgtaaccgcc cagctggtta
     3001 gccggttgcg agcagatcgg ccagctcgac gtcgtgccag cgcggtccgt gcgctgtaat
     3061 gcgcacccgg cgggtctcgc caactgcttc ggcggcccag tcgatgtcga cgtcgagccg
     3121 gtctggcgcg agcccttcga cgagacctct gccccactcg acgacggtca cagactcatc
     3181 gaccgaggcg tcgagatcga ggtcgtcgat ctcgagcgcg cccgctatcc ggtaggcgtc
     3241 gacatgcacc agcggcgggc ctgcggtctt gggtggatgc acgcgggcga tcacgaacgt
     3301 gggcgatgtc acgttgccgc gaacacccaa gcccgccccg attccctggg ccagcgtcgt
     3361 cttccccgcg ccgagggggc cagtgagcac gacaaggtcg ccagcccgca gtagcgcagc
     3421 gaggttcacg ccgacgcggt gcatggcgtc ggcggttgcg acgttcaggt cgacggtcat
     3481 gacttcggcg tgacgcccgc ggggcgatcg acagcagcga ccgggcgctc gacggcagcg
     3541 acagagcgat caaccaggcg ggcgacgaac gccaggtctt ctcgcagtgc ggcgcgcggc
     3601 atcccggccg gcccgaacct gatcgcggcc gaaggatggt aagtcggcac cacccgccgc
     3661 ccgtcaacgt catgggcacg gccgcgcagg tctgcgatct tggcccgctc gccgaagaac
     3721 cagcgggcgg cggtaccgcc aagggcgcag atcacctgtg gatcggcgac cgcgatctgg
     3781 gcggtcaacc aaccgcggca gttgagcacc tctgccttcg ccggctggcg attgcccggc
     3841 ggccggcact tcaagacgtt cacgaccgcg acactcgggc gcggcaggcc tacctcgccg
     3901 agcaactgat caagcagcgc gccagccttg ccgacgaacg gcagcccgct cgcatcttct
     3961 gtcgcacccg gtgcctcacc gacgagcagc accgccgcgc cagacggcgg cgcagttccg
     4021 acgacgacgt tcgtgcgggt tgctgcaagt tcggtgcagg cgacacacgc tcggatctgc
     4081 gtcgcgagct tggcccactc ggcctcagac acccctagac acccctagac accctcgtac
     4141 acccgcggaa cccgtacgcc gatccgagtg acgatctcgt acgaaatcgt gtcgagagcg
     4201 tccgcccaat cctgcgcggt cggctcgccg cgatcgccag ggccgaacag cacgacctcg
     4261 tcacccgccg cgaactcgtc agtatcggcg ccgccgagat cgaggacgaa ctggtccata
     4321 caaacggtgc ccgcgatcgt gcgacgaacg tcgcccgacc gaccacgcac cagcacctcg
     4381 gccttgttcg cagccgacct accgatgccg tcggcgtacc caagaggcac caggccgagg
     4441 gtggtcgccg ccgacgtgat gtaacgatga ctgtaagaaa cccctgtgtc cgcaggcact
     4501 ttcttggtca acgccagtcg ggctgtgagg gtcatcgcag gccgaagccc gagcgccgcc
     4561 gcgtcgcctt cgtcgggcgc cggcgagagg ccgtagatgg cgagaccggc gcgcaccagg
     4621 tcgaagcgcg tgtcaggtac gaagagcaag ccgccagagt tcgcaaggtg ccgcacctcg
     4681 ggcgagacac ctacccgcgt cgcgtagtcg acagcgtcgg agaacgcggt gacttgcaaa
     4741 cccgtcgtcg gatgccgcgg ctcgctgcca tgaacgagat gcgaccagag gccgacgatg
     4801 ttgacgactc catcagcctg tgcgcgcaga gcagcatcga cgagggccgg ccaatcgtcg
     4861 gcaacggcac ccccgcgcga gagcccggtg tcgaccttga gatgcacgcg agcaacccgg
     4921 tcgacgctcg ccgcagcagc agcgatctca cccatcatcc aaggggcgga cgccgacagg
     4981 tcgacgtccg cgagaactgc ctcggcaagt ggctcccccg gcgtcgcaag ccagcacagg
     5041 atgcgcccgt cgatgccgcc ggcgcgaagt gcgagcgcct cctcgaggaa cgcaacgccg
     5101 agccaggtcg ccccgccggc ccgagcagct tgcgcggtct gcacgaggcc gtgaccgtaa
     5161 ccctcagcct tcactacggc catgacgtcg gcgggccccg cgacatcggc gatgcgcgcg
     5221 acgttggccg agatgacgcc gagatcgatg cgggccgctg cccggagagc tccagaggcg
     5281 agcgaggaat cgctgaacgt cacggcgaca tcctgccagt gcgtgcgacg tcgtgatgtg
     5341 atggcagtca tgcagctcac caaatacacc cactcgtgcg tacggctcga cgacgaggat
     5401 cgcgccctcg tcatcgatcc cggcatgttt tccgagacag ggcccgccct cgacggagcc
     5461 cacgctgtgc tcatcaccca cgagcacgcg gaccacatcg acgccccggc gctgatccag
     5521 gcggccaagg ccgacgacgc actccgtatc tgggcaccgg ccgcggtcgc gcactcactc
     5581 gtcgaactcg gcgaccaggt gaccgcagta gacgcgggtg agtcgttcga cgcggccggg
     5641 ttcgcgatca cgacgttcgg cggcttgcac gcgctgatcc atcccctggt cgccgccccg
     5701 gtcgccaacg tctgctacct ggtcaacggc accgtctttc accccggaga ttcgttcacg
     5761 gttccaccag tgccggtcga gacgctgctg ataccaatta acgcgccatg gtcgaagatc
     5821 ggtgaggtga tcgacttcgc cgctgcggtc agggcgccgc gcgcgttcca gatccacgac
     5881 gcgctactga acgatcgcgg ctcgggtctg gtcgacagcc tcgtcggccg cgtcagtgcg
     5941 ctgtacggga ctacctacga gcgcctcgca gcaacccaaa ccgtcgacgt ctagccgagc
     6001 gaccggatcg cggccggcag gcatccgacg atgtcctctg ccgtgatcgg tgc
//
LOCUS       DASWVF010000746         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_225110, whole genome shotgun sequence.
ACCESSION   DASWVF010000746 DASWVF010000000
VERSION     DASWVF010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225110"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VGD55_13160"
     CDS             <1..1220
                     /locus_tag="VGD55_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEY0871343.1"
                     /translation="TLGDWLTNAGRITGLLAGYVIIILLLLMARVPSLERGLGADRLA
                     RWHAMGGRYAVSLSVAHTLLIIWGYAVSAHESVIPQTATLLTRYPDIMMATVALGLLV
                     LVAGVSMRAARARMRYETWYYLHFYTYLAVALSFSHEFATGIDFSSSQSARVAWSVLY
                     ISIVAVLVWYRFVVPIRAAVRHRMVVAGVFPEAPGIFSVHVTGHHLDDLRAEPGQFFR
                     WRFMDRGSWWQSHPYSLSAPPTDGLLRITVKVLGDHSHALRTLHKGTRVFAEGPYGAL
                     TERRRTRRQVLLLAGGIGVTPLRALFETLPARPGDLTLLYRAATEVDIVFRRELDAIA
                     AWRGARVLYLVGPPGSDADPFTGRRLAAVVPDLTAHDVYLCGPPRMMDAAHAALRRVG
                     VRRRQIHTESFEF"
     gene            1250..1906
                     /locus_tag="VGD55_13165"
     CDS             1250..1906
                     /locus_tag="VGD55_13165"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY0871344.1"
                     /translation="MRRFVAVFVATVAGLVVLLSFKETPTKAPRPLALAGPTAGGSSD
                     PASPGAVSSDPPTSSAAVGSVFSSAPATSAAATSTAAPSTSRSAATPSAKKASPTPTK
                     SSAKPTPNPTPKPSPTPSSATKTVTGSSVTVSEGRRVFGAVQVRLTLTNGKITAATAI
                     NYPNNDSRSSQISSFSIPILSQEVLQAQGSSIAVVSGATYTSNAYAQSVQAALDAAKA
                     "
     gene            1908..2681
                     /locus_tag="VGD55_13170"
     CDS             1908..2681
                     /locus_tag="VGD55_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0871345.1"
                     /translation="MPRPLVHVEHVMGTVVSFDVRADDSRRALMQDAVADAVSWLHRV
                     DEVFSTYRSDSQISRLGRGDLKLAECDDDVAEVLDLCAEIGRESDGYFSSTYGGQLDP
                     TGLVKGWAVQRASEILVGAGSEHHCVNGGGDIQAVGGMAVDSPWVIGISHPLQRDGFA
                     SVVTITGGAVATSGTAERGAHVVDPHTGQAVTSLASVSVVGADLIRSDAYATAAIAMG
                     GSARAWLESRSGYEAFAVAADGSGWWTSGYPVVGSVPAT"
BASE COUNT          426 a          874 c          916 g          521 t
ORIGIN      
        1 acacgctcgg ggactggctc acgaacgcgg gccggatcac gggacttctc gccggctacg
       61 tcattatcat cttgctgttg ctcatggcac gggtgccgtc gctcgagcgc ggtcttggtg
      121 ccgatcggct cgcgcgctgg catgcgatgg gtgggcgcta cgccgtttcg ctgtcggtcg
      181 cacacacgct gctcatcatc tggggctacg ccgtctcagc acacgagtcg gtgataccgc
      241 agaccgctac gttgctcacg cgctaccccg acatcatgat ggcgactgtc gccctcgggc
      301 tcttagtgct ggtcgccgga gtatcgatgc gcgccgcgcg cgcccgcatg cgttacgaga
      361 cgtggtacta cctgcatttc tatacctacc ttgcggtggc gctctcgttc agtcacgagt
      421 tcgctaccgg catcgacttc agcagcagtc agagtgctcg cgttgcgtgg agcgtgctct
      481 acatcagcat cgtcgcggta cttgtctggt accggttcgt cgttccgatc cgtgcggcgg
      541 tgcggcaccg catggtggtc gccggtgttt tccctgaagc cccaggaatc ttttccgtcc
      601 atgtgacggg ccatcacctc gacgatttgc gcgcggagcc tgggcagttc ttcagatggc
      661 ggttcatgga tcgtggcagt tggtggcagt cgcatccgta ttcactgtcg gcgcccccaa
      721 ccgacgggct gctgcgcatc acggtgaagg tgctcggcga ccactctcat gcgttgcgca
      781 cgttgcacaa aggcacgcgc gttttcgcgg agggtccgta tggcgctttg accgagcggc
      841 gacgtacccg ccggcaggtg ctgctgctag cgggcggcat cggcgtcacg ccattgcgtg
      901 cgctcttcga gacgttgcct gcgcggccgg gcgacctcac gttgctgtat cgggcagcga
      961 cggaggtcga catcgtgttc cgtcgcgagc tcgacgccat cgccgcgtgg cgcggtgcgc
     1021 gcgtcctgta tctcgtcggg cctcccggca gtgatgcaga tccttttacc ggacgccgat
     1081 tggcggccgt tgttcctgac ctgaccgcac acgacgtcta cctctgcggg ccgccgcgca
     1141 tgatggacgc cgcgcacgca gcgttgcgcc gggtcggcgt gcgccgacgc cagatccaca
     1201 ccgaatcgtt tgagttctaa tgaaccgcgt catagaagga gggtgaacca tgcgccgctt
     1261 tgttgccgta ttcgttgcga ccgtcgcggg tctcgtcgtc ctgctgagtt tcaaggagac
     1321 gccgaccaag gcaccgcggc cgctggcgtt ggctggcccc acggcgggcg gttcgtcgga
     1381 tccagcttcg cccggagcag tttcgtcaga cccgccgaca agctccgctg ccgtcggctc
     1441 cgtgttctcg agcgcgccag caaccagtgc ggccgcgaca tcgacggcag ctccgtcgac
     1501 gtctcgttct gccgccaccc cgagcgcgaa gaaggcgtca cctacgccga cgaagtcatc
     1561 ggcgaagccc acccctaacc cgaccccgaa accgtcaccg acgccgtcca gcgcgacgaa
     1621 gacggtgacc ggtagctcgg tgactgtgag cgagggccgc cgggtgttcg gcgcagtgca
     1681 ggttcggctg acgctgacca acgggaagat caccgccgct accgcaatca actatccgaa
     1741 caacgattcg cggtcgtcgc agatcagctc gttctcgatt cctatcttga gtcaagaagt
     1801 gcttcaggcg cagggatcct cgatcgccgt cgtgtcaggg gcgacctaca cgagcaacgc
     1861 gtatgcgcag tctgtgcagg cggctctcga cgcagcaaag gcgtgacgtg ccaagaccgc
     1921 tggtgcacgt cgagcacgtg atgggcacgg tggtcagctt cgatgtgcgg gccgatgatt
     1981 cgcggcgcgc actcatgcag gacgcagtcg cggatgccgt gagctggctg caccgggtcg
     2041 acgaggtgtt cagcacctac cgatccgaca gccagatttc caggctcggt cgaggcgact
     2101 tgaaattggc agaatgcgac gacgatgtcg ccgaggtgct tgacctgtgc gcggagatag
     2161 gtcgcgaaag cgacgggtac ttcagctcga cctacggcgg gcaactcgat ccgaccgggt
     2221 tggtgaaggg ctgggccgtg cagcgggcga gcgaaatcct tgttggcgcg gggtccgaac
     2281 accactgcgt caacggcggc ggcgacattc aggctgtggg cggcatggcg gtcgacagcc
     2341 cttgggtcat cggcatctcc caccctttgc aacgcgacgg atttgccagc gttgtcacga
     2401 tcaccggtgg cgcggtcgcg acgtccggca ccgccgagcg gggtgcgcac gtggtcgatc
     2461 cgcacaccgg gcaggcggtg acgagccttg ccagcgtgtc ggtggtcggc gctgacctga
     2521 ttcgctccga cgcctacgcc accgctgcga tcgcgatggg tggctcggca cgcgcgtggc
     2581 tggagtcgcg tagcggctac gaagctttcg cggtcgcagc ggacggatcg ggctggtgga
     2641 cgtcggggta tccggtggtc ggcagcgttc cagcgacctg acggtgtgtg ctgtcagcga
     2701 gagcgctcgc cgagcggcaa cctgaacaca atgtccg
//
LOCUS       DASWVF010000747         6424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_21176, whole genome shotgun sequence.
ACCESSION   DASWVF010000747 DASWVF010000000
VERSION     DASWVF010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21176"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGD55_13175"
     CDS             complement(<1..191)
                     /locus_tag="VGD55_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871346.1"
                     /translation="MAASSGSARGGLTAVASFLRVYEPLEAFPADERAWWRAVAANGP
                     LTASQMAAREHAAALRALAA"
     gene            390..632
                     /locus_tag="VGD55_13180"
     CDS             390..632
                     /locus_tag="VGD55_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEY0871347.1"
                     /translation="MGRGRAKAKQTKVARELKYSSGGTDLDALRKELASAASTPATST
                     PSWSKDEADDADEADDFGDADEDDGLDDSYGASGSR"
     gene            complement(629..1684)
                     /gene="purM"
                     /locus_tag="VGD55_13185"
     CDS             complement(629..1684)
                     /gene="purM"
                     /locus_tag="VGD55_13185"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0871348.1"
                     /translation="MTDRVTYAAAGVDIEAGDRAVELMKTSVNRASRPEVVGGLGGFA
                     GLFRFDATKYRSPLLATSTDGVGTKVAIAQALDKHDTIGIDLVGMVVDDLVVCGAEPL
                     FMTDYIACGKVVPERIAAIVGGIAEGCRQAGCALIGGETAEHPGLLSPDEYDLAGAGT
                     GVVEEDDVLGAHRVRAGDVVIAMASSGLHSNGYSLARHVLLTRGGLRLDQHVDELGTT
                     LGEELLVPTRIYSLDCLALINAVDVHAFSHVTGGGLAANLDRVLPVGVDIEIDRTTWA
                     APPIFGLIQRLGDVATPELERTLNMGVGMVALVAEADVDRALDVLRARGVTAWGAGRA
                     RAGEGPGTTTLVGTHAG"
     gene            complement(1698..3233)
                     /gene="purF"
                     /locus_tag="VGD55_13190"
     CDS             complement(1698..3233)
                     /gene="purF"
                     /locus_tag="VGD55_13190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0871349.1"
                     /translation="MPRGDGRLNHDLDPDENGPQDACGIFGIWAPGEEVANLTYFALY
                     ALQHRGQESAGIAVSDGRKILVYKDMGLVSQVFNESNLASLHGDIAVGHTRYSTSGSS
                     VWENAQPTFRSTAVGSIALAHNGNLTNTGDLAQLVEKAQADSGEIAFDHRPLRSTSDT
                     DLVTSLLASHPDRSLETAAVEVLRQVEGAFSFVFMDETTLYAARDRYGVRPLALGKLE
                     HGWVVASETAALDIVGASYVREIEPGELLAIDENGPRSVSFAEPDPKFCVFEFVYLAR
                     PDSSFDGRSVHATRVELGRQLAREAPAEADLVMPTPDSGTPAAIGYAEESGIPYAAGL
                     VKNAYVGRTFIQPSQTLRQLGIRLKLNPLRDVIRGKRLVVVDDSIVRGNTQRAVVRML
                     REAGATEVHIRISSPPVKWPCFYGIDFATRAELIANGLTIEEIRTSIGADSLSYVSLE
                     GLVTATNRPKANLCRACFDGEYPTVLPDPQFMGKQLLEITPVDALMRTVPSVGAHTAL
                     EHP"
     gene            complement(3300..3839)
                     /locus_tag="VGD55_13195"
     CDS             complement(3300..3839)
                     /locus_tag="VGD55_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0871350.1"
                     /translation="MELHAPAGGVLIVPMSREEFDALPEMPHTEWWDSACVMTGGTTY
                     RHARAVMAMGGLLGVALREGDRRDFEVLAGVGWRLPDAEFAPDIAVVAPAPLDTLLSS
                     PPLLVVEVLSRSTRHIDLGRKRQLYAAGGLAWYWFVDLANDELVVLRNDEGSFIEVQR
                     LTSGTTLGPVEISVTVADL"
     gene            complement(3859..4215)
                     /locus_tag="VGD55_13200"
     CDS             complement(3859..4215)
                     /locus_tag="VGD55_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871351.1"
                     /translation="MKTSWYDEIAQGVRAQCRLDDEGGTWVLFLDEQRRPMNAMALPH
                     EELGPLDFDGLASVVDDVDAPAVIVAVPRWDGQPRAEDWRLWEELRSRMAVARSELID
                     LVVVGERSWWAATGAT"
     gene            complement(4308..5819)
                     /locus_tag="VGD55_13205"
     CDS             complement(4308..5819)
                     /locus_tag="VGD55_13205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0871352.1"
                     /translation="MDLSDTHSASHPAATQLRWSPALWAALIVLCGVVFLDALDVSMV
                     GMALPSIQRDLKLSTDQLQWIVSGYVLGYGGLLLLGGRAADLLGRRRVLLWALSVFVI
                     ASALGGLTTNGDLLITARFIKGASAAFTAPAALSIITTTFAEGPVRNRALSIFTTVGA
                     SGFSLGLVLGGLLTELGWRWTFLLPAPIAAGLVVLGRKYVARDTHTRSAGGYDVPGAV
                     TASAAMLLLVRTVVTAPASGWGSVSTLGGFVVSALLLGAFIAIELRSPHPLVRLGILR
                     SSRLVRANIAAMALFGSYIGFQFIGTLYLQSLLGWSSLQTAFAFLPAGVIVAFGSTRI
                     APIIDRFGTPRLLAIAFAVLTTGYALFIRLGASASYVTLMLPTVVLLGIGFAVGFPSL
                     NVQATNGVADDEQGLASGLLNTSFQLGGAIGLAIVTAVVTSHTGVGTGSKAVLDGFRP
                     GIVVATGIGMFGLLVSLSGVWSERRTRVTQIEDVADDDDGSSYEEFALTAQDR"
     gene            5898..6272
                     /locus_tag="VGD55_13210"
     CDS             5898..6272
                     /locus_tag="VGD55_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0871353.1"
                     /translation="MPGTDGPVVDQWRSLLEQHARVTCALEKALGEHGLGVSEFEVLD
                     ELASQPQSECRMQALSDVVHLSQSALSRVVGRLEEEGLVTRVMCQQDRRGIFAHLTDE
                     GRSRYEAALPTHREVLAENLHA"
BASE COUNT         1186 a         2145 c         2104 g          989 t
ORIGIN      
        1 gccgccagcg cgcgcagggc cgcagcgtgt tcgcgggctg ccatttggga cgccgtcagc
       61 gggccattcg ccgcgaccgc gcgccaccac gctcgctcgt cggccgggaa ggcctcaagc
      121 ggctcgtaga cccgaaggaa cgaggcgacc gcggtcagcc caccgcgggc cgacccggaa
      181 ctggcagcca ccctcgcatc gtcgcacgac cgggagccgc acggccgaaa tggggctccc
      241 gcgtcgtgat caacgggggt gcctgcaacg gtttcgaacg ggtacgctgt accgacggcc
      301 tcggcgggaa cgcccgggcc tagctactgt gtatgagtct gtacatgtcg tccgatgcgg
      361 ggcaacggcc ccgacgaggg ggtcgagcca tggggcgcgg ccgggccaaa gccaagcaga
      421 cgaaagtcgc ccgcgagctg aagtattcaa gcggcgggac cgacctcgac gctttgcgca
      481 aagaactcgc gagcgcggcg tcgacgcctg ccaccagcac accaagctgg agcaaggacg
      541 aagctgacga tgctgacgaa gctgacgact tcggcgacgc ggacgaagac gacggcctcg
      601 acgacagcta cggagcctcc ggctcccgct agcccgcgtg cgtccccacc agcgtggtgg
      661 tgccggggcc ctcgcctgct cgagcgcggc cagctcccca agccgtaaca ccgcgtgcgc
      721 gcaacacgtc gagcgcacgg tcgacgtccg cctcagccac caacgcaacc atcccgacgc
      781 ccatgttcaa cgtccgctcc agctcaggcg ttgcgacgtc gccaagacgc tggatcaaac
      841 cgaagatcgg cggggccgcc cacgtcgtcc gatcgatttc gatgtcgacc ccaaccggca
      901 acacccggtc gaggttcgcg gccaacccgc cacccgtgac atgcgaaaac gcgtgtacgt
      961 cgaccgcgtt gatcagggcg agacagtcga ggctgtagat gcgggtaggc acgagcagtt
     1021 cctcgccgag cgtcgtaccg agttcatcca cgtgttgatc aagacgcaga cccccgcggg
     1081 tcagcagcac gtggcgggcg agtgagtagc cgttcgagtg cagcccactc gatgccatcg
     1141 caatcacgac gtcgccggcc ctgacccggt gcgcgccgag cacgtcgtcc tcttcgacga
     1201 caccggtgcc agcacccgca aggtcgtatt catcgggcga cagcagcccc gggtgctccg
     1261 cggtttcgcc gccgatcagc gcgcagccag cttgccggca tccttcggcg atgccgccga
     1321 caatcgcggc gattcgctcg ggcacgacct tgccgcatgc gatgtagtcg gtcatgaaca
     1381 acggctctgc accacagacc acaagatcgt cgaccaccat gccgacgagg tcgatgccga
     1441 tcgtgtcgtg cttgtcgagc gcctgcgcga tcgcaacctt ggtgccgacg ccgtctgtcg
     1501 atgtcgcaag cagcgggctg cggtacttgg tggcgtcgaa gcggaacagg cccgcgaatc
     1561 cgccgagccc gcccacaacc tcagggcgcg aggcgcggtt caccgaggtc ttcatcagtt
     1621 cgacggcgcg atcgccagcc tcgatgtcga cccccgcggc ggcgtaggtg acgcggtctg
     1681 tcacggccgt ttcgcggtca cggatgttcg agggcggtat gtgcgcccac cgaaggcacc
     1741 gtacgcatca acgcatcgac tggggtgatc tcaagcagtt gcttgcccat gaattgcggg
     1801 tcgggcagca cggtcgggta ttcgccatcg aagcacgcac ggcacagatt ggccttcggc
     1861 cggttcgtcg ctgtcaccaa cccctcgagc gagacgtacg acagcgagtc ggcaccgatc
     1921 gacgtccgga tctcttcgat cgtcaggccg ttcgcaatca gttcggctcg cgtcgcgaag
     1981 tcgatgccgt agaagcacgg ccacttcacc ggcggcgacg agatgcggat gtgcacctcg
     2041 gtcgctccgg cctcgcgcag catgcggacg acggctcgct gggtgttgcc tcggacgatg
     2101 gagtcgtcga cgacgacgag gcgcttgccg cggatcacgt cgcgcaacgg gttcagcttc
     2161 agccgaatgc cgagttggcg cagcgtctgc gacggctgga tgaacgtgcg accgacgtag
     2221 gcattcttga caaggcctgc ggcataaggg attccggact cttcggcgta gccgatcgcc
     2281 gcgggtgtgc ctgagtcggg tgtcggcatc acgaggtcgg cctcggccgg cgcttcgcgt
     2341 gcgagctgtc gaccgagctc gacccgcgtg gcatgcacgc tgcgcccgtc gaaggacgag
     2401 tcggggcgcg cgagatagac gaactcgaag acgcagaact tcggatcggg ctcggcaaaa
     2461 ctcacagatc gcggcccgtt ctcatcgatc gcgagcaact cgcctggctc gatctcgcgc
     2521 acgtaggacg cgccgacgat gtcaagtgcg gcggtctcgc tggcaacaac ccaaccgtgc
     2581 tcgagcttgc caagagccaa tggccggacg ccgtagcgat cgcgagcggc gtacagcgtc
     2641 gtctcgtcca tgaaaacgaa actgaaggcg ccttcgacct gccggagcac ttcgacggcg
     2701 gcggtctcga ggctgcggtc ggggtggctg gcgagcaacg acgtgacgag gtcagtgtcg
     2761 gacgtcgacc gcagcggtcg gtgatcgaac gcgatctcgc cgctgtcggc ctgggccttc
     2821 tcgacgagct gcgcaagatc accggtattg gtcaggttgc cgttgtgcgc aagcgcgatg
     2881 ctgccgacgg cggtcgagcg gaacgtgggc tgcgcgttct cccacaccga tgagccagac
     2941 gtcgagtagc gggtgtggcc aacggcgata tcaccatgca ggctggcgag attcgactcg
     3001 ttgaacactt gtgagaccag gcccatgtcc ttgtacacaa ggattttccg gccgtcactg
     3061 accgcaatgc cagcggactc ctggcctcgg tgctggagtg cgtagagggc gaagtaggtg
     3121 aggttagcta cctcttcacc gggggcccag atgccgaaga tgccgcacgc gtcctgcggg
     3181 ccgttctcgt cagggtccag atcgtgattg agacggccgt cgccacgagg cacctgcggg
     3241 gctccttgcg agagtgattc gcgggagtgg atcaatggtc cgttaggtct cgcagtgtac
     3301 tagagatcgg cgacggtaac cgagatctcg acagggccaa gcgtcgtccc gctggtgagt
     3361 cgttgtacct cgatgaagct gccctcgtcg ttgcgcagga ctacgagctc gtcgttcgca
     3421 agatcgacaa accagtacca ggccaggcca cccgcggcgt agagctggcg cttgcgaccc
     3481 aggtcgatgt gacgcgtcga cctcgagagc acctcgacca cgagcaacgg ggggctgctg
     3541 agcaacgtgt caagcggcgc gggcgcgacc accgcgatgt cgggcgcgaa ctcagcgtcc
     3601 ggcagtcgcc agcccacacc tgcgaggacc tcgaaatcgc ggcgatcacc ctcgcgcaac
     3661 gcgacgccca ggaggccacc catggccatg accgctctgg cgtgccggta ggtcgtgccg
     3721 ccggtcatga cgcacgcact gtcccaccat tcggtgtgcg gcatctcggg caacgcgtcg
     3781 aattcctcac gcgacatcgg cacaatgagg acgccaccag cgggtgcgtg cagctccatg
     3841 atgggcactt taccgcgatc acgtcgcacc ggttgccgcc caccatgacc gctcaccgac
     3901 cactacgagg tcgatcagct cggaccgcgc gaccgccatc ctgcttcgca gttcctccca
     3961 caagcgccag tcttcggcgc gcggttgacc gtcccatcgc ggcactgcca cgatcacggc
     4021 tggagcgtcg acgtcgtcga cgaccgacgc caggccgtcg aagtcaagcg gcccgagctc
     4081 ttcgtgcggg agtgccatcg cgttcatcgg acgtcgttgt tcgtcgagga agagcaccca
     4141 cgtgccaccc tcgtcgtcga gcctgcattg cgctctgaca ccctgcgcga tctcgtcgta
     4201 ccagctcgtc ttcatgtgcg tggagtctgc tgctccgaac acgtacgcgg gtggccgtcg
     4261 gcggtcgctg tggacaactt ccgccgaggc gcacccgcgt gtgtgggcta cctgtcctgt
     4321 gccgtaagcg cgaactcctc gtaggacgat ccgtcgtcat catcggcgac gtcctcgatc
     4381 tgcgtgacgc gcgtgcgtcg ttcgctccag actccggaca gcgagaccaa cagcccgaac
     4441 atgccgatcc ccgtcgcgac cacgatgccc gggcggaacc cgtcgagcac cgccttcgag
     4501 ccagttccga cgcccgtatg actggtcacg accgcagtca cgatcgcgag gccgatggcg
     4561 ccgccaagct ggaacgaggt gttgagcagg ccagatgcca agccctgctc gtcgtcggca
     4621 acgccattcg ttgcctgcac gttcaacgac gggaacccga ccgcgaagcc gatgccgagt
     4681 aggacgaccg tcggcagcat cagggtcacg tacgacgcgc tcgcaccgag acggatgaac
     4741 agtgcgtatc cggtcgtcag taccgcgaac gcaatcgcca gcaaccgggg ggtgccgaac
     4801 cggtcgatga tcggcgcgat gcgggtcgac ccgaaggcga cgatcacgcc cgccggcagg
     4861 aacgcgaacg ccgtctgcaa agacgaccag ccaagcagcg actgcaagta gagcgtgccg
     4921 atgaactgga acccgatgta cgagccgaac aacgccatcg ccgcaatgtt cgcgcgcacg
     4981 agacgcgacg accgcaggat tccgaggcgg accagcgggt gtggtgaccg cagttcgatc
     5041 gcgatgaacg caccaagcaa caacgcgctc acgacgaatc cgccaagagt gctgaccgat
     5101 ccccagccgg acgccggggc ggtgacgacc gtccgaacga gcagcagcat cgcggcactc
     5161 gccgttacgg ctcctggcac gtcataacca ccggcactgc gagtgtgcgt gtcgcgcgcg
     5221 acgtacttgc gacccaggac gacgaggccc gcggcgatcg gcgccggcag caggaacgtc
     5281 cagcgccagc cgagttcggt gagcaacccg ccaaggacga ggccgagcga aaagccgctc
     5341 gcgccgaccg tggtgaagat gctgagtgcc cggttacgca ccggaccctc ggcgaaggtc
     5401 gtggtgatga tcgacagggc cgcgggggcg gtgaaggccg cgctcgcgcc cttgatgaag
     5461 cgggccgtga tgagcagatc gccgttcgtg gtgaggcccc cgagagccga ggcgatcacg
     5521 aagacgctca atgcccacaa gagcacccgc cgccgtccaa gcaagtctgc tgctctgccg
     5581 ccgagcagta ggaggccgcc gtagccgagc acgtagcccg aaacgatcca ctggagttgg
     5641 tcggtgctga gtttgaggtc gcgctggatc gaggggagcg ccatgccgac catcgacacg
     5701 tcgagtgcat caaggaagac gacgccgcac agcacgatga gcgcggccca cagggccggg
     5761 ctccagcgca attgggtggc agcggggtga ctggctgagt gagtatctga aaggtccatg
     5821 cgacgacaat acatgcacgt gcattgattg caagagaata ttttgcgctg gtcatatttg
     5881 cgcggtaccg ttaccacatg cctggcaccg atggtcccgt cgtcgatcag tggcgttctt
     5941 tgctcgaaca gcacgcgcgc gtcacgtgcg cgctcgagaa ggcgctcggg gagcacggtc
     6001 ttggagtcag cgagttcgag gtcctcgacg aattggccag ccagccacag tccgaatgcc
     6061 gcatgcaggc gctatccgac gtcgtccacc tgagtcaaag tgcgctctcg cgggtcgtcg
     6121 gtcggctcga agaagagggc ctcgtgaccc gcgtcatgtg ccagcaagat cgccgcggca
     6181 tcttcgcgca cctgaccgac gagggccgtt cccggtatga ggcagcactg ccgacccacc
     6241 gcgaagtgct cgccgagaac ctgcacgcct aaatcagcga cgcggccacc gaaccgactc
     6301 gttcgccgcc gcctaaagaa ccggcagcag cccgctgatg tcaccgcgct cgccactggc
     6361 gctgagccgg ccgtcggcga gcgcctcact ccacgacagc cggcccgccg cgagccgtac
     6421 ccag
//
LOCUS       DASWVF010000748         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_505276, whole genome shotgun sequence.
ACCESSION   DASWVF010000748 DASWVF010000000
VERSION     DASWVF010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505276"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGD55_13215"
     CDS             <1..307
                     /locus_tag="VGD55_13215"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871354.1"
                     /translation="DMTAYTLHLGFFSSALLFAAAISVPALAYRFTGLNAIAAFWCAY
                     VLTRPLGASLADWMGTPHKLGGLNWGRGNVSVGLTLLIVIGVALLARRTPVSGDVNA"
     gene            complement(304..>1917)
                     /locus_tag="VGD55_13220"
     CDS             complement(304..>1917)
                     /locus_tag="VGD55_13220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY0871355.1"
                     /translation="LYSRAVNTGQPLSDVIAIVAPWCRELTESIAAMCKEYVVRKRAL
                     GLVDFDDLLLYWRAAALDPIVGQQLAAEFDHILVDEYQDVNALQVDVVAALRSSDRRL
                     TVVGDDAQAVYGFRAADPAHLLDFELAFPDATTITLETNYRSSPQILQAANAVGAQAP
                     AGFRAILRAAEPERSNGNRPQLLRCADEDAQSNAVCERVLEHREAGLRLRDQAVLFRA
                     AHHSEHLELELSRRAIPYVKYGGLRYLEAAHVKDLLAAFRLADNTRDEVSWFRLLQLL
                     DGVGPATARRVIDALALFEDKTEADADVLARWAGASALLPARVRAAADAAVAALASHV
                     GETVGVHAERLRVAIAPLIEAAYPDAMSRIADLDVLVSAAGSCARLADVAADYALEPP
                     RSTGDFAGPPLIDEDWLILSTVHSAKGLEWDVVHLLHAADGNFPSDMALGTAQGLEEE
                     RRLFYVAMTRPRRALNVYVPLRYHHHPRAHDDAHSWSQPSRFLAGDVTHYFDEQWIEN
                     PDLVLPTANMTAVVPAADVVAGQLDALWA"
BASE COUNT          344 a          622 c          619 g          332 t
ORIGIN      
        1 agatatgacg gcgtacacac tgcaccttgg cttcttctcg tccgcgttgt tgttcgccgc
       61 agcgatcagt gttccggccc tggcctatcg gttcaccggg ctgaacgcaa tcgcggcgtt
      121 ctggtgcgcg tacgtactca cccgcccgct tggcgcgtcg ctcgctgact ggatgggcac
      181 cccacacaag ctcggtggcc tcaactgggg cagaggaaac gtgagtgtcg ggctgacgct
      241 cctgatcgtc atcggcgtcg cgttgctagc tcgccgcacc ccggtgagcg gggacgtgaa
      301 cgcttaagcc cacagtgcgt cgagttgccc ggccaccaca tcggcggccg gcaccacggc
      361 agtcatgttc gccgtcggca acacgagatc aggattttcg atccactgct cgtcgaagta
      421 gtgcgtgacg tcgccagcca ggaatcgtga cggctgcgac cacgagtgtg cgtcgtcgtg
      481 cgcgcgtgga tgatggtggt accgcagcgg aacgtagacg ttcagggctc ggcgcggtcg
      541 ggtcatggcc acataaaaga ggcgtcgttc ctcctcgagg ccctgtgccg ttccgagcgc
      601 catgtcagag ggaaagttgc cgtcggccgc gtgaagcaga tgcacgacgt cccactcgag
      661 acctttcgcc gaatgcacgg tcgacaggat cagccagtcc tcgtcgatga gtggcggccc
      721 tgcgaaatcg ccggtcgagc gcggcggctc gagcgcatag tcggccgcga catcggccag
      781 tcgcgcgcac gatccagctg cacttacgag cacgtcgagg tcggcgatgc gcgacatcgc
      841 gtccgggtag gcggcttcga tcaacggcgc gatggcgacg cgcaaccgtt ccgcgtgcac
      901 gccgactgtc tcacccacat gcgacgcgag agctgcaaca gcggcatcgg cagctgctcg
      961 tacccgcgca ggcaacaatg ccgacgcacc tgcccatcgc gccagcacat cggcgtccgc
     1021 ctcggtcttg tcttcaaaca acgccagtgc atcgataacg cgacgcgcgg tcgctggacc
     1081 cacgccgtcg agtagttgga gcagtcgaaa ccacgacacc tcgtcgcggg tgttgtcggc
     1141 gagcctgaac gcagcgagca gatccttgac gtgggcggct tcgagatagc gcaagccgcc
     1201 gtacttcacg tacggaatcg cgcgccggct gagttctagc tcaaggtgtt cagaatggtg
     1261 cgcggctctg aacaacacgg cctgatcgcg cagccgcagg cccgcctcgc gatgctcaag
     1321 cacccgctcg caaacggcat tgctctgcgc atcttcgtcg gcacagcgca ggagttgcgg
     1381 ccggttgccg tttgaacgtt ccggctcggc ggcgcgcaag atcgctcgaa agcccgctgg
     1441 cgcttgcgca ccgacggcgt tcgctgcttg cagaatttgc ggcgacgaac ggtagttcgt
     1501 ttccagcgtg atcgtcgtcg cgtcgggaaa tgcgagctcg aagtcgagca gatgggccgg
     1561 atcggcggcc cgaaatccgt agaccgcctg tgcatcatcg cccacgacag tgagccggcg
     1621 atcagaggaa cgcagcgccg caaccacgtc gacctgcagc gcgttcacgt cttggtattc
     1681 gtcgaccagg atgtggtcga actcagccgc gagttgctgg ccgacgatcg ggtcaagcgc
     1741 agcggcgcgc cagtagagca gcaagtcgtc gaagtcgacg agaccgagcg cgcgctttcg
     1801 cacgacgtac tccttgcaca tagcagcgat cgattcggtc aactcgcggc accacggcgc
     1861 gacgatcgcg atcacgtcgg acagcggctg cccggtgttc accgctcgcg agtacag
//
LOCUS       DASWVF010000749         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_428701, whole genome shotgun sequence.
ACCESSION   DASWVF010000749 DASWVF010000000
VERSION     DASWVF010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428701"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="hydA"
                     /locus_tag="VGD55_13225"
     CDS             complement(<1..1152)
                     /gene="hydA"
                     /locus_tag="VGD55_13225"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY0871356.1"
                     /translation="MTILVKNGTVVSPTGAIAADVLIDGERIVALLEPGTTKLGVDLE
                     ASADVVIDARDKYVVPGGVDGHTHMQMPFGGTQASDTFETGTRAAAWGGTTTIIDFAI
                     QRTGERVLDGLAAWHEKADGNCAIDYGFHQIIGGVDDESLKAMDELVSEGITSIKLFM
                     AYPGVFYSDDGQIVRAMQTAAANGSLVMMHAENGIAIDVLVAQALARGETAPRYHGLT
                     RPWETEEEATHRAIMLARMTGAPLYIVHMSAKQAVATLAAARDDGWNVFGETCPQYLY
                     LSLEEHLSAPDFEGAKYVCSTPLRSRAEGHQNELWKALRTNDLSVVSTDHCPFCMKEQ
                     KELGIGNFAKIPNGIGGVEHRMDLIYQGVVDGHITLPRWVELCSTTPARM"
     gene            complement(1161..1523)
                     /locus_tag="VGD55_13230"
     CDS             complement(1161..1523)
                     /locus_tag="VGD55_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871357.1"
                     /translation="MPVVTDVDADLPDRGVEDRKAEIAGPEVELLPEAVDVREVVLAV
                     LAEVFAVGVEDLGDDDFYGTTYFVDPEGKFVGDVGDPYSPDLVVRDLDMDLLTEVRNR
                     WAFYRDRRPEAYGDLTRP"
     gene            1416..>1822
                     /locus_tag="VGD55_13235"
     CDS             1416..>1822
                     /locus_tag="VGD55_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0871358.1"
                     /translation="MNGFWEKFYFRPGNLGFPVFDTAIGKIGVYICYDRHFPEGWRAL
                     GLNGAQIVFNPSATSRGLSSYLWKLEQPAAAVANEYFIGAINRVGVEDLGDDDFYGTT
                     YFVDPEGKFVGDVGDSYSPDLVVRDLDMDLLTEV"
BASE COUNT          311 a          573 c          576 g          362 t
ORIGIN      
        1 catccgggcc ggcgtcgtcg agcacagttc gacccagcgc ggcagagtga tgtggccgtc
       61 aacgacacct tggtagatca gatccatccg gtgctcaacg ccgccgatgc cgttgggaat
      121 cttggcgaag ttgccgatgc cgagttcttt ctgttccttc atgcagaacg ggcagtggtc
      181 agtgctgacg accgagaggt cgttggtgcg cagcgctttc cacaactcgt tctggtgccc
      241 ttcggcgcgt gaacgcaacg gcgtcgagca cacgtacttc gcgccttcga agtctggcgc
      301 agagaggtgt tcttcgagcg acaggtagag gtactgaggg cacgtctcgc cgaagacgtt
      361 ccaaccgtcg tcgcgggctg cggccaacgt cgcgaccgcc tgcttggccg acatatgcac
      421 gatgtagagc ggcgcacccg tcatgcgggc gagcatgatc gcgcggtgag tggcttcttc
      481 ctcggtctcc cacggcctgg tgagtccgtg gtagcgcggc gcggtctcgc cgcgcgcgag
      541 ggcctgcgca acaagcacgt cgatggcaat gccgttctct gcgtgcatca tgaccagcga
      601 accgttggcc gcagccgttt gcatcgcgcg cacgatctgg ccgtcgtcgg agtagaaaac
      661 gcctggatac gccatgaaca gtttgatgct ggtgatgcct tcgctgacca gttcgtccat
      721 cgctttcagc gattcgtcgt cgacgccgcc gatgatctgg tggaatccat agtcgatcgc
      781 gcagttgccg tcagcttttt cgtgccacgc agccaggccg tcgagcactc gctcaccggt
      841 gcgctgaatc gcgaagtcga tgatcgtcgt ggtaccgccc cacgccgcgg cgcgggtgcc
      901 ggtctcgaag gtgtctgacg cctgcgtgcc gccgaacggc atctgcatgt gcgtgtgacc
      961 gtcgacaccg cctggcacga cgtacttgtc gcgagcatcg ataacgacgt cggcgctcgc
     1021 ttcgagatcg acgccgagct tcgtcgtccc cggttccaga agagcgacga tgcgctcgcc
     1081 gtcgatcagg acgtcagcag cgatcgcacc ggtcggactc acgaccgtgc cgttcttcac
     1141 caggatcgtc atgttgctcc ctaaggccgg gtgaggtcgc cgtacgcctc gggtcggcgg
     1201 tcgcggtaga acgcccagcg attgcgcacc tcggtcaaca ggtccatgtc gaggtcacgc
     1261 acgacgagat cgggtgagta cgggtcgccg acgtcaccga cgaacttgcc ctcggggtcg
     1321 acgaagtacg tagtgccgta gaagtcgtcg tcgccgaggt cttcgacgcc gacggcaaat
     1381 acctcggcaa gtaccgcaag caccacctcc cgaacatcaa cggcttctgg gagaagttct
     1441 acttccggcc cggcaatctc ggctttccgg tcttcgacac cgcgatcggg aagatcggcg
     1501 tctacatctg ttacgaccgg cactttccgg aaggctggcg cgcactcggg ctgaacggtg
     1561 cgcagatcgt cttcaatccg tcggccacgt cgcgcgggct ttcgtcgtac ctctggaagc
     1621 tcgagcagcc ggccgcggct gtggcgaacg aatacttcat cggggccatc aaccgggtcg
     1681 gcgtcgagga tctcggtgac gacgatttct acggcaccac gtacttcgtt gatcctgagg
     1741 gcaagttcgt cggggacgtc ggcgattcgt actcacccga tctcgtcgta cgcgatctcg
     1801 acatggacct gttgaccgag gt
//
LOCUS       DASWVF010000750         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_71620, whole genome shotgun sequence.
ACCESSION   DASWVF010000750 DASWVF010000000
VERSION     DASWVF010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71620"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>1784
                     /gene="lepA"
                     /locus_tag="VGD55_13240"
     CDS             51..>1784
                     /gene="lepA"
                     /locus_tag="VGD55_13240"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0871359.1"
                     /translation="MSVRTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRA
                     QYLDRMDIERERGITIKSQAVRLPWQVEGTGYVLNMIDTPGHVDFTYEVSRSLAACEG
                     AVLLVDAAQGIEAQTLANLYLAIQSDLHIIPVLNKIDLPAAQPERYAEELAHVIGCEP
                     GDVLRVSAKTGFGVRELLDHVVHHVPHPIGDFDAPPRALIFDSVYDVYRGVVTYVRVV
                     DGYLSSRERIKMLSTGTTHELLEVGVISPEPIPTEGLGVGEVGYLITGVKDVRQSRVG
                     DTVTVVSRAATEPLAEYTLPKPMVYSGLYPIDGSDYPLLRDALDRLQLNDAALAYEPE
                     TSAALGFGFRCGFLGLLHLEIVRERLEREFNLDLISTAPNVVYRVVREDGKEITVTNP
                     SEYPEGKIMEVFEPVVRATVLSPSDYVGTIMELCQSRRGNLLGMDYLSADRIELRYTM
                     PMAEIAFDFFDQLKSRTRGYASLDYEPTGEQAGDLVKVDILLQGEAVDAFSAIVHKDK
                     AYAYGVEMTKKLRELIPRQQFEVPIQAAIGSRIIARESIRAIRKDVLAKCYGGDISRK
                     RKLLEKQKEGKK"
BASE COUNT          334 a          580 c          583 g          287 t
ORIGIN      
        1 cccggacgcc gtcagcgggc ggccgccccg gcttcatggg accatgagct gtgtccgtcc
       61 gtaccgatcc agcgctgatc cgcaacttct gcatcatcgc ccacatcgac catggcaagt
      121 ccacgctcgc cgaccggatg ctccagctga ccggagtcgt cgacgagcgg tcgatgcgcg
      181 cccagtatct cgatcggatg gacatcgagc gcgagcgcgg catcacgatc aagagccagg
      241 ccgtcaggct gccgtggcag gtcgagggca ccggctacgt gctgaacatg atcgacacgc
      301 ccggtcacgt cgacttcacc tatgaggtgt cgcggtcgct cgcggcgtgt gaaggtgccg
      361 tgctgctcgt cgacgcggcg cagggcatcg aggcgcagac gctggccaac ctgtacctgg
      421 cgatccagag cgacctgcac atcatcccgg tgctgaacaa gatcgacctg cctgcggccc
      481 agcccgagcg gtacgccgaa gagctcgcgc atgtcatcgg ctgcgagcca ggcgacgtcc
      541 tgcgagtaag cgctaagacc ggcttcggcg tgcgcgagct gctagatcac gtggtgcacc
      601 acgtcccgca cccgatcggc gacttcgatg cgccgccacg tgcgctcatt ttcgactcgg
      661 tctacgacgt ctatcgcggc gtcgtcacgt acgtgcgggt cgtcgacggc tacctgtcga
      721 gccgcgagcg catcaaaatg ctttcaaccg ggacgacgca tgagctgctc gaggtcggcg
      781 ttatctcacc agaaccgatc cccaccgaag ggctcggagt cggcgaggtc ggctacctca
      841 tcacgggcgt caaggacgtt cgccagtcgc gggtcggcga cacggtgacg gtcgtgagcc
      901 gggccgcgac cgagccgctc gccgagtaca ccctgccgaa gccgatggtc tactcgggcc
      961 tgtatccaat cgacggcagc gactatccgc tgctgcgcga cgccctcgac cgcctgcaac
     1021 tgaacgacgc cgcgctggcg tacgaacccg agactagcgc ggcgctcggg ttcggtttcc
     1081 gctgcggatt cctcggcctg ctgcatctgg agatcgtgcg cgagcggctc gagcgcgagt
     1141 tcaacctcga cctgatctcg accgcgccga atgtcgtgta ccgggtggtg cgcgaagacg
     1201 gcaaagagat cacggtcacg aacccgagcg aataccccga gggcaagatc atggaggtgt
     1261 tcgaaccggt tgtgcgcgcg accgtgctgt ctccgagcga ctacgtcggc acgatcatgg
     1321 agttgtgcca gagcaggcgc ggcaacctgc tgggcatgga ctacctctcg gccgaccgga
     1381 tcgagttgcg ctacacgatg ccgatggccg agatcgcatt cgacttcttc gaccagctga
     1441 agtcgcgcac ccgcgggtat gcgagcctcg attacgagcc cacgggtgag caggccggcg
     1501 acctggtgaa ggtcgacatc ctgcttcagg gtgaggcggt cgacgcgttc agcgcgatcg
     1561 tccacaagga caaggcgtac gcgtacggcg tcgagatgac caagaagctg cgcgaactca
     1621 tcccgcgcca gcaattcgag gtgccgatcc aggcggcgat cgggtcgcgc atcatcgcgc
     1681 gcgagagcat cagggcgatc cgcaaggacg tgctcgcgaa atgctacggc ggcgacatca
     1741 gccgaaagcg caagcttctt gagaagcaga aggaaggcaa gaag
//
LOCUS       DASWVF010000751         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_352404, whole genome shotgun sequence.
ACCESSION   DASWVF010000751 DASWVF010000000
VERSION     DASWVF010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352404"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2140)
                     /locus_tag="VGD55_13245"
     CDS             complement(59..2140)
                     /locus_tag="VGD55_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0871360.1"
                     /translation="MSYRQSPLRPRGGGRRRSRGPLIVVLVVVLAGAAVAGYYLYPRS
                     SKGTANPGAAASSEPTISASASHLPPTDALVLQFTQAWTKGNLTDVSYLGGLTGADVQ
                     SAYAAIVAPLHAVSVAVTAQPAQPTADDHVSQSALNVTWQLPGGQVWTYESSVNVNEV
                     DGSWLLAWKPAIVNPALGAGDVLQYTRLAPTRATILDGAGQPIITARPVVDIGLEAGN
                     VVNVNSTSNQMAAILGVNAAALATRIRGSSADEFVDVITLREPDFEKVAAAINAIPGV
                     ILRNLTEPLSPSHDFARSLIGTVGPVTKEIVDASNGRYVSGDVGGLGGLEKDFDSVLG
                     GTPGYEIDVVHPASPPTVLHVQAAVAGTSLQTTLDQQVEAAADAALGSVTTQPSALVA
                     VRVSTGQVIAVANGPAGGGFDTALLGQIVPGSAFKIVTTTALLEQGFNVNTSVPCLPQ
                     ILVEGKTFHNYEGEQLGSVPFHTDFAKSCNTAFISLSSKVGGKTLPDTAKSLGIGACW
                     SLGTPAFAGSVALPGDQVDLAATSFGQGSTLLSPVSLAVAAATVARGSYLAPQLVLNG
                     TPSSCAAPASAAAPLNPAVVSHLHSLMREVVTAGTGAVLAKAPGGPVMAKTGTAEYGP
                     GATPKTHAWLVGYQGDIAFAVYVQDGQSGGTVAAPVALAFLQNLAGAQSASESPKASP
                     SHPSPKASASH"
     gene            2353..2925
                     /locus_tag="VGD55_13250"
     CDS             2353..2925
                     /locus_tag="VGD55_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871361.1"
                     /translation="MTVVPDEHTTSTVAAPMKTRHRIVAIAVAVVFVAIAVFEVRFYV
                     VPSSNRRGPTDAVVVLGGGTFIDRLRAAGALLQQYPGAVEVVSTPGRQGCPYPYPGAS
                     DIICFRPNPATTQGEARATAALAAQYGWKHITVVTTADQVWRARLRFSRCWSGDLSVV
                     RAPTSIATRLFSAPYETAATIKAEVFQRGC"
BASE COUNT          512 a         1033 c         1016 g          520 t
ORIGIN      
        1 ttagatgcac gttcaactag tcgggaagct taccgccgtc cctcactggt cgtgccggct
       61 agtggctcgc tgacgccttc gggcttgggt ggctgggtga cgccttcggg ctctcggacg
      121 ccgactgcgc gcccgccagg ttctgcagga acgcgagcgc gaccggcgcg gcgacggtcc
      181 caccactttg gccgtcttgg acgtagaccg cgaaggcgat gtcgccctgg tagccgacca
      241 gccaggcgtg cgtcttcggt gtggcgcccg gtccgtattc agccgtgccg gtcttcgcca
      301 tcaccgggcc gcctggcgcc tttgcgagca ccgcgcccgt gccggcggtc acgacctcac
      361 gcatgagcga atgcaggtgg ctgacgacgg ccgggttcaa cggggcagca gccgacgcgg
      421 gcgctgcaca actcgacggc gtgccattca gcactagctg aggagcgagg tagctgccgc
      481 gcgcaacggt tgcagcggcg acggcgagac tgaccgggct cagcagggtc gagccttgcc
      541 cgaacgaggt agcggcgagg tcgacctggt cacccggcaa cgctactgag cccgcgaacg
      601 cgggtgtgcc aagcgaccaa cacgcgccga tcccgaggct cttcgcggta tctggcaacg
      661 tcttcccacc gaccttcgac gacaggctga tgaaggctgt gttgcacgac ttggcgaagt
      721 cggtgtggaa cggcacgctg ccgagttgct cgccctcgta gttatggaac gtcttgccct
      781 cgaccagaat ttgcggcagg cacggcaccg acgtgttgac gttgaaacct tgctcgagca
      841 gtgctgtcgt cgtcacgatt ttgaatgccg aaccgggcac gatttgccca agcagtgcgg
      901 tgtcgaagcc accacccgcc ggcccgttgg cgacggcgat gacttgtccg gtcgagacac
      961 gcacggccac gagtgcactc ggctgcgtcg tgacgcttcc gagcgcggca tcggcggccg
     1021 cttcgacctg ctggtcgagc gtcgtctgca gcgacgtgcc ggcgacggct gcttgcacgt
     1081 ggagcacggt cggcggcgac gcaggatgaa caacatcgat ctcgtatccc ggagtgccgc
     1141 cgagcaccga gtcgaaatcc ttctcgagac cgccgagacc gccgacgtcg cccgacacgt
     1201 aacggccgtt cgacgcatcg acgatctcct tggtcactgg cccgactgtg ccgatcaacg
     1261 accgcgcaaa gtcatgcgac gggctcagcg gttctgtcag gttgcgcaaa atgacaccgg
     1321 gaatcgcgtt gatggcagca gcgaccttct cgaagtcggg ctctcgcagc gtgatgacat
     1381 cgacgaactc gtcagccgaa gaccccctga tccgcgtcgc aagtgcggcg gcattgacgc
     1441 cgaggatcgc cgccatctgg ttcgaagtgg agttgacgtt cacgacattg cccgcctcga
     1501 ggccgatatc gaccacgggt ctggccgtga tgatcggctg cccggcaccg tcgaggatcg
     1561 tcgcgcgcgt tggcgcgagc cgggtgtact gcagcacgtc gccggcaccg agtgcgggat
     1621 tgacgatcgc cggcttccac gcgagcaacc agctgccgtc gacttcgttg acgttcacac
     1681 tcgactcgta ggtccacacc tgaccgcccg gcagctgcca ggtgacgttg agcgctgatt
     1741 gggacacgtg atcgtcagcc gtgggctgtg cgggttgtgc tgtgaccgcg accgaaaccg
     1801 cgtgcaaagg cgcgacgatc gcggcgtacg cgctctgcac gtcggcccct gtgagcccac
     1861 ccagatacga cacgtccgtc aggtttccct ttgtccacgc ctgggtgaac tggagaacga
     1921 gggcatcggt cgggggcaga tgagaggcac tcgccgagat cgtcggctct gacgacgcag
     1981 ctgcaccggg gttcgccgtc cccttcgaac tgcgtgggta caggtagtag cccgcgactg
     2041 ccgcgccagc aagaaccacg acgaggacga cgatcagcgg gccgcgggaa cggcgccggc
     2101 cgccaccgcg cggccgcagc ggcgattgcc ggtagctcac gagccgagcc taatgggagc
     2161 ggcggcgaca ccgtcagcgg cgaagcaggt gcgagacgcg cgttcaggtt gccttcaggt
     2221 cggcgcggtt aacctttagg tatctgcggg cagcgtccga tgctcgtagc cagtggctgc
     2281 tagcggtgat cgctgcccct agcaacccct agctgcccaa cgcgaaggtg gattgctccc
     2341 ggtggtcacg ctatgacagt tgtgcccgac gagcacacga cctcgaccgt ggccgcgcct
     2401 atgaagaccc gccaccggat cgtcgcgatc gccgtcgccg tcgtgttcgt ggccatcgca
     2461 gtgttcgaag tccggttcta cgtcgtcccg tcgtctaatc ggcgcggccc aaccgatgcc
     2521 gtcgtcgtgc tcggcggcgg cacgttcatc gaccgactgc gtgccgctgg cgcactcctc
     2581 cagcaatacc ccggcgcggt cgaagtggtc tccacaccgg gccgccaagg gtgcccgtac
     2641 ccctatccgg gcgcatcgga catcatctgt tttcgtccca acccagccac gacccaaggc
     2701 gaggcacggg caaccgccgc gctcgccgcg cagtacggct ggaagcacat caccgtcgtc
     2761 accaccgctg accaggtgtg gcgcgcgagg ctgcggttca gccgctgctg gtcgggggat
     2821 ctgagcgtgg tgcgcgcgcc gacgagcatc gcgacgcgat tgttctccgc gccctacgag
     2881 accgccgcca cgatcaaagc cgaggtattt caacgcggct gttaaggctg tcggcccgac
     2941 aatccgagca acttgtcgcc cgcgtcatcg ctgtcgaccg gcaccggtgc cgcgaagatg
     3001 cccgccgcgt gccagtcctt tgcgttggct tcgaggtacg ccagcaccac agccgcagca
     3061 gccggatcga tcgactcgtc g
//
LOCUS       DASWVF010000752         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_352440, whole genome shotgun sequence.
ACCESSION   DASWVF010000752 DASWVF010000000
VERSION     DASWVF010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352440"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..908
                     /locus_tag="VGD55_13255"
     CDS             21..908
                     /locus_tag="VGD55_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0871362.1"
                     /translation="MAGTQIAQRTRANPQIECAPDDGLVRDHLPLVGYLVAEAASRLP
                     GHVSRDDLTSAGLMALAQSARSFDASRGVPFARYASMRIRGGIIDELRSHDWASRSVR
                     IKARQRGVAEEALAATLRRHPTQKELANYLGVSLVELAAVERDVHRSVVLSLQGFAEA
                     GTLEGMLPHQDPGPEEMLLDRERESYLVDAVAALPERLRVVVQGYFFDERPMVDIAAE
                     LGVTESRISQMRAEGLAMLKAGMTAMLAPEQLETADRPDGCVARRKAAYYAAIAASSG
                     YAARVSLSATAATRIAGAA"
     gene            1100..1354
                     /locus_tag="VGD55_13260"
     CDS             1100..1354
                     /locus_tag="VGD55_13260"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEY0871363.1"
                     /translation="MIVLTRLNGPSFALNPDLIERVEANPGTVVVLIGGASYPIAESV
                     ADVLDRVRDYRSQVIASAHAVEFPPVTERKLHSLPTSDAE"
     gene            1544..2224
                     /locus_tag="VGD55_13265"
     CDS             1544..2224
                     /locus_tag="VGD55_13265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0871364.1"
                     /translation="MMPNGMETLALNAPVLVRLADGQDHEYAARIADFDVDEILISAP
                     AGGSAALLASGAREVDVAWLSRRGRYEQRCVIVDHAAVVQVGQKQSSLQKLWRLRPVR
                     RPVLIQRRRHIRVTAKLPVRVVIGSNASAGVTIDISEGGFRVRLPRQHLPDLAHATVH
                     ATVGAARVAVSGYVMRSIDAPPNHTDAVIAFDAAGTDADAIRRLVLNAKLRSKTTRTA
                     SARSPRSR"
     gene            complement(2226..2603)
                     /locus_tag="VGD55_13270"
     CDS             complement(2226..2603)
                     /locus_tag="VGD55_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871365.1"
                     /translation="MNEVHGEFDAVPSSVAEARRFARAALEAGGAAGDGWPVEQVVSE
                     LATNAVVHAGTSFVLSIAQDDSHIRVSVTDARPLARATIRRFSEQTTTGRGLRLIQSL
                     GQAWGVDQTDSAKTVWCELRRDA"
     gene            complement(2862..3599)
                     /locus_tag="VGD55_13275"
     CDS             complement(2862..3599)
                     /locus_tag="VGD55_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY0871366.1"
                     /translation="MEVAEHVDVLIAQGRALFDAARAASMDAVVPPCPGWTVRDLVTH
                     VGGVHRWAAAIVGGALSDSDDATGGQVGKGPDDAQLLEWFRDGHRALVQTLQEAPSDL
                     ACFTFLPAPSPLAFWARRQAHETAIHRADAEAVGGVVSVFDTEFARDGIDEILRGFAS
                     RPRPEMQSATLKVQLDAVPDTWVIRFGPRGLTVSTDAARALVADLTVSGSASSVYLWL
                     WNRPAEVSLVGDPRVVELWRQIRVRWS"
     gene            3677..>4141
                     /locus_tag="VGD55_13280"
     CDS             3677..>4141
                     /locus_tag="VGD55_13280"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0871367.1"
                     /translation="MVKPSDAGNIGLLRLIAQRLAGPGFDDAADTVRWMTAIQAQDYA
                     GAVLSVALRTASRDRKAVYEALDSGAVVRSWTMRGTLHFVAAEDLSWMTTLTSERLIA
                     RAVKLHDHLGIDAKTIGRAREVAIENLSGHRRLTRTQLLAHWEKAGFGDVKQQ"
BASE COUNT          757 a         1390 c         1307 g          687 t
ORIGIN      
        1 gtcgcggcga tgactgaggc atggcaggca cccagatcgc tcagagaacc cgcgcgaatc
       61 ctcagatcga atgcgccccc gacgatggcc tcgtcaggga tcacctcccg ctcgtgggat
      121 acctcgtcgc cgaggccgcg agccggctcc ccggccacgt gagtcgcgac gacctcacgt
      181 cagcgggctt gatggccctc gcccagtcgg cccgcagttt cgacgcgagc cgtggcgttc
      241 ctttcgcccg atacgccagc atgcggatcc gcggcggcat catcgatgag cttcgcagcc
      301 acgactgggc cagccgatcc gtgcgcatca aggcgcggca gcgcggcgtt gccgaagagg
      361 cattggccgc aaccctgcgc cgccacccga cccagaaaga actcgccaac tacctcggcg
      421 tctcactcgt cgaactcgct gcggtcgagc gcgatgttca tcggtcagtt gtccttagcc
      481 tgcaaggttt cgctgaggcc ggcacgctcg aaggcatgct gccccaccag gatccgggcc
      541 cggaggagat gcttctcgac cgcgagcggg agtcctacct cgtcgatgcg gtggccgcgt
      601 tgccagaacg actacgagtc gtcgtccagg gctacttctt cgatgaacga ccgatggtcg
      661 atatcgccgc cgagcttggc gtcaccgaat cccgcatctc gcagatgcga gccgaagggc
      721 tcgcaatgct gaaggcgggc atgaccgcga tgctcgcacc cgagcagctc gagacggccg
      781 accgtccgga cggatgcgtc gcccgccgca aggccgccta ctacgccgcc atcgcggcaa
      841 gctccgggta tgccgcacgg gtcagcctga gcgccactgc cgcgacaagg atcgcgggcg
      901 ccgcataagg gtgtgccgct tggaccgacc cgcccgctag gccagaagag cgaaagcccg
      961 cgacagctaa agtcgcgggc ttcttgcttt ctcaggttgg ctctcgaccg gccgaaaggg
     1021 acgcaggcca ccgatgtggc cactcgggaa ctgggccggc acgcagccgg tgagaacccg
     1081 tcgcgaatgg agtattcgca tgatcgtgtt gacccggctg aacgggccct cgttcgcgtt
     1141 gaaccccgac ctgatcgaac gtgtcgaggc caatcccggc accgtagtgg tcctcatcgg
     1201 aggcgcaagc tatccaatcg ccgagtccgt cgctgatgta ctcgaccggg ttcgcgacta
     1261 ccgcagccaa gtcatcgcct ccgcccacgc cgtggaattc cctcccgtca ccgagcgcaa
     1321 gcttcacagc ctgcccacat cggacgccga gtagcttcca cccgcccctt gcagcccact
     1381 tcgcatccct ggtggtcgtg aaataagccc cggcagtcgg aaaccgcagc cactcgggag
     1441 gcgaagtggt gtgaagtagc gcagaaaatc gcccttgggt gcttgcctcc ctcggcgccg
     1501 atgccgagac gttcgtcgtg aacccgcgcc caggcgaccg ccgatgatgc cgaacggcat
     1561 ggagacgctc gcgctgaatg cgccggtgct ggtgcgcctt gccgacggcc aagaccacga
     1621 atacgcggcc cgcatcgccg atttcgacgt cgacgagatc ctgatcagcg caccggccgg
     1681 gggcagcgcc gcactgcttg cgagcggtgc ccgtgaggtc gacgttgctt ggctctcgcg
     1741 ccgtggtcgt tacgagcagc gctgtgtcat cgtcgaccac gccgcagttg tgcaggtcgg
     1801 ccagaagcag tcgagccttc agaagttatg gcgccttcga cccgttcgac ggccggttct
     1861 catccaacgc agacgtcaca tcagggtcac agcgaaactg ccggtgagag tcgtgatcgg
     1921 ctcgaacgct tcggcgggcg taacgatcga tatctccgag ggcgggttcc gggtgaggtt
     1981 gccgcgtcag catctgccgg atctcgcgca cgcaaccgtg cacgcgacgg tcggggcggc
     2041 gcgggttgct gtctcgggtt acgtcatgcg ctcgatcgac gcgccgccca accacaccga
     2101 cgcagtgatc gcgttcgatg cggcgggtac tgatgccgac gcgatccgac ggctggtgct
     2161 taacgcgaaa ctgcgaagca agaccacgcg gacggcgtcg gctcggtcac cgcgatcgcg
     2221 ctagttcacg cgtctctgcg gagttcgcac cagacggtct tggctgagtc ggtctgatcg
     2281 acgccccacg cttggcccag gctttgaatc agccgcagac cgcgacccgt tgttgtctgc
     2341 tcagagaacc tgcgaatcgt cgcgcgagcg agcggcctgg catcagtcac cgacactctt
     2401 atatgtgaat cgtcctgcgc gatgctcagc acgaacgatg taccggcgtg cacgacagcg
     2461 ttcgtcgcca attcgctgac gacctgttcg actggccaac cgtccccggc cgcaccgcca
     2521 gcttcgagtg ccgcgcgcgc gaatctacgc gcctcggcga cactgctcgg cactgcgtcg
     2581 aactcgccgt ggacctcatt cacgctggtg atcttacaaa cggaaccacg gcgcagaatc
     2641 gcccaaccgg cttgagaaac gccgaccgaa cgggcggact taacctgaag accgcgttgc
     2701 cttcttggcg tccgggtgag actccgatcg aacagcgcgc ggtctggggg gcgtggcgat
     2761 gttcgtgggg cgcggctgcc cggcggagca cgagggggac accgccgggg ccgctccctt
     2821 tcactgcggc gacttcgtaa aggcgtggcg cggcgtacct gctagctcca acggacacgg
     2881 atctgtcgcc acagttccac gactcgtggg tcgccaacga gcgaaacctc cgccgggcga
     2941 ttccacagcc atagatacac gctcgacgcg ctgccgctga ccgtgaggtc ggcaacaaga
     3001 gctcgcgctg catcggtcga gacggtgagc ccccgggggc caaacctgat gacccacgtg
     3061 tctggaaccg cgtccagttg cactttcaag gttgccgact gcatctccgg ccgtgggcgc
     3121 gaggcgaagc cgcgcaggat ctcatcgata ccgtcgcgtg cgaactcggt gtcaaacacc
     3181 gagacgacgc cacccacggc ctccgcgtcg gctcggtgaa tcgccgtctc atgagcttgg
     3241 cggcgcgccc agaaggccag tggcgacggt gcgggaagga acgtgaaaca cgcgagatcg
     3301 cttggagcct cctgcagcgt ctggacaaga gcacggtgcc cgtcgcggaa ccactcaaga
     3361 agctgtgcat cgtcggggcc ctttccgacc tgcccgccag ttgcgtcgtc gctgtcggaa
     3421 agcgcgccgc cgacgatggc ggcggcccat cgatgaacac cgcccacatg cgtgaccagg
     3481 tcgcgaaccg tccaaccagg gcagggcggg acaaccgcat ccatcgacgc tgctctggct
     3541 gcatcgaaca atgcgcggcc ttgggcaatc agcacatcga cgtgctcagc gacctccacg
     3601 ccgcacattg aacctcagac gtatgacccg acgaaacagc gatgcccaga acgcactcac
     3661 gtgttgcaat gagcgaatgg tcaaaccgtc tgacgcgggc aacatcgggc tgctgcggct
     3721 gattgcacaa cgccttgctg ggcctgggtt cgatgacgcg gccgacaccg ttcgctggat
     3781 gacggcgatc caggcacagg actacgcagg tgcggtcctg tcagtcgctt tgcgcactgc
     3841 atcgcgcgat cgcaaggctg tctacgaagc tctggactct ggcgccgtgg tgcggtcgtg
     3901 gaccatgcgt ggcacgttgc atttcgttgc ggctgaagac ctttcgtgga tgacgacgct
     3961 cacatccgag cggcttattg cacgagcagt gaaactccac gaccacctcg gcatcgacgc
     4021 aaagacgatc gggcgagctc gcgaagtagc aatcgagaat ctgtctggcc ataggcgtct
     4081 cactcgcaca caactgcttg cccactggga gaaagccggc ttcggtgacg tcaagcaaca
     4141 g
//
LOCUS       DASWVF010000753         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_454774, whole genome shotgun sequence.
ACCESSION   DASWVF010000753 DASWVF010000000
VERSION     DASWVF010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454774"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2511
                     /locus_tag="VGD55_13285"
     CDS             112..2511
                     /locus_tag="VGD55_13285"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY0871368.1"
                     /translation="MTAVEDRPVGTEDFVNNDLKPVGHGRMLRKEDPRFIRGMGHYVD
                     DIQLPGMLHLAILRSPVAHARIVKIDVSAAQAHPKVKAVITGADLAAKGLAWMPTLSN
                     DVQAVLATDKVRFQGQEVAFVVAEDRYAARDALELIDVEYDILPPVIDVRQALAPDAP
                     LIRDDLEGKKDNHCFDWQTGDEAATAAVFAKADVVVTEDIVYPRVHPAPMETCGCVAD
                     FERVAGKLTLWTTSQAPHAHRTVYALVSGLPEHKIRVIAPDIGGGFGNKVPIYPGYVC
                     AIVASLVIGKPVKWMEDRSENLVSTGFARDYIMRGEIAATKEGKILAIRTNVLADHGA
                     FNGTAAPIKYPAGFFGVFTGSYDLEAAFCKMTAVYTNKAPGGVAYACSFRITEAVYLV
                     ERIVDCLAYELKMDPAELRLKNFIKPEQFPYTTKTGWVYDSGNYEPAMRLAMKMAGYE
                     DLRKEQAEKRARGELMGIGVAFFTEAVGAGPRKDMDILGLGMADGSELRIHPTGKAVL
                     RVSCMSQGQGHETTYAQIVAEEIGIPPADIQVVNGDTDNTPFGLGTYGSRSTPVSGAA
                     AALVSRKVRDKAQIIASGMLEVSVADLEWEKGSFHVKGDPSKSVTIQEIAFRAHGAGD
                     LPPGVEGGLEAQICYNPSNLTYPFGAYICVVDIDPGTAQVKVRSFVAVDDCGTRINEM
                     IIEGQIHGGLTDGVGMALMEMIAFDEDGNCLSSSLMDYLIPTALEVPEWQTGHTVTPS
                     PHHPIGAKGVGESATVGSPPAIVNAIVDALKPFGIRHADMPLTPSRVWETMQGRHRPP
                     V"
     gene            2508..3410
                     /locus_tag="VGD55_13290"
     CDS             2508..3410
                     /locus_tag="VGD55_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0871369.1"
                     /translation="MTNETRGRQRLTDRAADLAARREPFVHATVVRAQSPTSARPGDD
                     AIVTSDGAIEGFVGGQCVKESVRTAALAALESGEPVLLRVLPDDSVSFPDTPGAQLTI
                     NPCLSGGALEIFLEPHLPSAVVEVVGDTPVADAVALFAEGVGFAVGRSLPGRPPEGAM
                     AVIVCSHGDDEPESIRAALDAGVAYVGLVASRSRGEAVLEAMDLSDGELARIHTPVGL
                     EIGAKTAAEIGLSIVADIVRAMRVNAVSAPRDESMPKPKTAVDPVCGMTVIVLPDTPY
                     LSVGGIDTWFCSPGCRDSYAAQVG"
     gene            3420..4040
                     /locus_tag="VGD55_13295"
     CDS             3420..4040
                     /locus_tag="VGD55_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0871370.1"
                     /translation="MVVTVLVLAAGSSQRLGRPKQLLPYRGTTLLDATLSVARSCGLD
                     QVMVTLGGAGDAVRSAVDLSGLDVVDNPDFGAGCGSSIAAAVELLDERTDGIALLLGD
                     QPGVSPATVRRLVDAAAGSPLGVCRYADALGHPFWFGREVFSDLRALHGDKAVWKLLE
                     SGRYPVIEVDAEGLVPLDVDTWDDYEALLARDAEARGVDKSQGVST"
     gene            3962..4927
                     /locus_tag="VGD55_13300"
     CDS             3962..4927
                     /locus_tag="VGD55_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0871371.1"
                     /translation="MGRLRGVARPRRRGARRRQEPGGVDIATPVRDAADIQARLDALD
                     YLVDEGLATALYLSIVLGRPLLLEGEPGVGKTTAAKALARALDVPLIRLQCYEGLTVG
                     EALYEWNYQRQLLAIRLAEATAETITDADLFTEEFLLERPILQAVRHRGPAAPVLLID
                     EIDRADDEFEALLFEFLGESSVTIPELGTFTAVQPPIVVLTSNRSRELHDALRRRCLY
                     HWIEFPSPERAAEILRRTVPSATELLIQTTTQFVGRVRALELDKPPGMAETIDWLSAL
                     SALGATELLRADVLLTLGAIAKTPDDRDTMVGALVELGYSGPSNP"
     gene            4956..>5399
                     /locus_tag="VGD55_13305"
     CDS             4956..>5399
                     /locus_tag="VGD55_13305"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0871372.1"
                     /translation="MKIENEFSVNVPVDEAWQVLTDLERIAPCMPGAQLTGREGDAFL
                     GKVKVKVGPVISEFAGTASFIEKNDATHHAVIDAKGRDSRGAGNASAIITAQLRADGD
                     HRTVVTVETDLKISGKLAQFGSGMIAEVSSKLLTQFVDSLEKQVMS"
BASE COUNT          994 a         1757 c         1752 g          896 t
ORIGIN      
        1 gggccagatc tgccgttgta cgggctacac gacgatcgtt cgctcgatca aatgggcggc
       61 cgcgaatgaa gccgctacga acgcagcagc cgccactgcg ggcgcgacag catgaccgcc
      121 gtcgaggacc gcccagtcgg caccgaagat ttcgtcaaca acgacctgaa gccggtcggc
      181 cacggccgca tgctccgcaa ggaagacccc cgattcatcc gcggcatggg ccactacgtc
      241 gatgacatcc agttgccggg gatgctgcac ctcgcgatcc tgcgctcgcc agtcgcacac
      301 gcgcggatcg tgaagatcga cgtcagcgcc gcgcaagccc acccgaaggt gaaggccgtc
      361 atcactggag ccgacctcgc cgccaagggt ttggcttgga tgccgacact gtcgaacgac
      421 gtgcaggccg tgctcgcgac cgacaaggtg cggttccagg gccaggaggt cgcgttcgtc
      481 gtcgcggagg atcggtatgc cgcgcgtgac gccctcgagt tgatcgacgt cgagtacgac
      541 atcctgcccc cagtgatcga cgttcggcag gcactcgcac ccgatgcacc actgatccgc
      601 gacgacctcg aaggcaagaa agacaaccac tgcttcgatt ggcaaaccgg cgacgaggca
      661 gcaaccgcag cggtgttcgc gaaggccgac gtggtcgtca ccgaggacat cgtctacccc
      721 cgcgtgcacc cggcaccgat ggagacgtgt gggtgcgtcg ccgacttcga gcgggtcgcc
      781 ggcaagctca cgttgtggac caccagtcag gcgccgcacg cgcaccgcac cgtctacgcg
      841 ttggtctcgg gcctgcccga gcacaagatc cgcgtgatcg cgcccgacat cggcggtggc
      901 ttcggaaaca aggtgccgat ctaccccggg tatgtctgcg cgatcgttgc gtcactcgtc
      961 atcggcaagc cggtgaagtg gatggaagac cgctcggaga acctggtcag caccggtttt
     1021 gcgcgcgact acatcatgcg cggtgagatc gcggcgacga aggaaggcaa gatcctcgcg
     1081 atccgcacga acgtgctcgc cgaccacggc gcattcaacg gcacggccgc gccgatcaag
     1141 taccccgccg gattcttcgg cgttttcacc ggcagctatg acctcgaggc cgcgttctgc
     1201 aagatgacgg cggtctacac gaacaaggcg ccgggcggtg tcgcctacgc atgctcgttc
     1261 cgcatcaccg aggccgtcta cctcgtcgag cggatcgtcg actgcctcgc ctacgagctc
     1321 aagatggatc cggccgaact gcgactgaag aacttcatca agcccgagca gttcccttac
     1381 acaaccaaga ccggctgggt ctacgactcg ggcaactacg aacccgcgat gcggttggcc
     1441 atgaagatgg ccggctacga agacctgcgc aaagagcagg ccgagaagcg ggcacgcggt
     1501 gagttgatgg gcatcggcgt tgcgttcttc accgaggccg tcggcgcagg tccgcgcaag
     1561 gacatggaca ttctcggtct tggcatggcc gacggttctg agttgcggat tcacccgaca
     1621 ggcaaggcag tgctgcgggt gtcgtgcatg agccaaggcc agggccacga gacgacctac
     1681 gcgcagatcg tggctgaaga gatcggcatc ccgccggcag acattcaggt cgtgaacggc
     1741 gacaccgaca acaccccgtt cggcctgggc acctacggca gccggtcgac accggtgtca
     1801 ggtgcagccg ctgcactggt ctcgcgcaag gtgcgcgaca aggcgcagat catcgcgtcg
     1861 ggcatgctcg aagtgtcggt cgccgacctc gagtgggaga aaggttcgtt ccacgttaag
     1921 ggcgacccct cgaagtcggt gacgatccag gagatcgcct tccgtgcaca cggtgcgggc
     1981 gacctgccgc ctggcgtcga gggtggactc gaagcacaga tctgctacaa cccctcgaac
     2041 ctcacgtacc cgttcggtgc ctacatctgc gtcgtcgaca ttgatccggg gacggcgcag
     2101 gtgaaggtcc gtagcttcgt cgcggtcgac gactgcggca cccgcatcaa cgagatgatc
     2161 atcgagggac agatccacgg cggtctcacg gacggcgtcg gcatggcatt gatggaaatg
     2221 atcgccttcg acgaagacgg caactgcctg agcagttcgc tcatggacta cctcatcccg
     2281 accgcacttg aagtacccga atggcagacc gggcataccg tgacgccatc cccgcaccac
     2341 ccgatcggtg cgaagggcgt gggtgagtcg gccaccgtcg gatccccgcc ggccatcgtg
     2401 aacgcgatcg tcgacgcgct gaagccgttc ggcatcaggc acgccgacat gccgctcaca
     2461 ccgtcgcggg tctgggaaac catgcaagga aggcaccggc cgccggtgtg acgaacgaaa
     2521 cccgcggacg tcaacgactc accgatcgcg cggccgacct ggccgcgcgg cgggagccgt
     2581 tcgtgcacgc cacagtggtg cgcgcgcagt ccccgacgtc cgcacgtcca ggcgacgacg
     2641 cgatcgtgac gagcgacggg gccattgagg gtttcgtcgg cgggcaatgc gtcaaggagt
     2701 cggtgcgcac tgccgcgctc gcggcgctgg aaagcggtga acccgtgctg ctgcgggtac
     2761 tccctgatga cagcgtgtcg ttccctgaca cgcccggtgc acaactgacc atcaacccct
     2821 gcctgtcggg aggtgctttg gagatcttcc tcgagcccca cctcccgtcg gccgtcgtcg
     2881 aggtggtcgg tgacacgccg gtcgctgacg cggtggcgct gttcgcggaa ggcgtcggat
     2941 tcgccgtcgg ccggtcattg ccggggcgcc caccagaggg cgcgatggcc gtgatcgtgt
     3001 gcagtcacgg cgacgacgaa cccgagtcga tccgggccgc actcgatgca ggcgtcgcct
     3061 acgtcggcct cgttgcgagc cgcagccgcg gagaagcggt gctcgaggcg atggacctca
     3121 gtgacggcga gctcgcgcgc atccacactc cggtcggcct cgagatcggt gcaaaaacag
     3181 cagccgagat cgggctctcg atcgtcgctg acatcgtgcg cgcgatgcgg gtcaatgccg
     3241 tgagtgcacc tcgagacgaa tcgatgccca agcccaagac cgcagtcgac cccgtgtgcg
     3301 gtatgaccgt gatcgtgctc ccggacactc cctacctgtc agttggcggc atcgatacct
     3361 ggttttgcag cccgggctgc cgcgacagct acgcggccca ggtcggctag ggcgcagctg
     3421 tggtcgtcac cgtgctcgtg ttggcggccg gatcttcgca acggctcggc cgccccaaac
     3481 agttgcttcc ctaccgcggc acgacgctgc tcgacgcgac gttgagcgtg gcgcgtagct
     3541 gcggtctcga ccaggtgatg gtgacacttg gcggggccgg tgacgccgtt cgatcggcgg
     3601 tggatctgag tggtctcgat gtcgtcgaca acccggattt cggcgcaggc tgcggatcat
     3661 cgatcgcggc cgcagtcgag ctgctcgacg agcgcactga cggcatcgcg ctcttgctcg
     3721 gcgaccagcc gggcgtatcg cccgcaactg tgcgacgact cgttgacgcg gctgccggtt
     3781 cgccgcttgg agtgtgtcgc tacgccgacg cgctcggaca cccgttctgg ttcggccgcg
     3841 aagtattcag cgacctgcgc gcgctgcacg gcgacaaggc ggtgtggaag ctgctggaat
     3901 ccggccggta tccggtgatc gaggtcgacg ccgaaggctt ggtaccactc gacgtcgaca
     3961 cgtgggacga ctacgaggcg ttgctcgccc gcgacgccga ggcgcgcggc gtcgacaaga
     4021 gccagggggt gtcgacatag ccacgccggt acgcgacgcc gccgacatcc aggcgcgact
     4081 ggacgcgctc gattacctcg tcgacgaagg tcttgcgact gcgctgtatc tctccatcgt
     4141 gctcgggcga ccgctgctcc tcgaaggaga gcctggcgtc ggcaagacga ccgccgcaaa
     4201 agcactcgcg cgcgcactcg acgtccctct tatccggttg cagtgttatg agggattgac
     4261 cgtcggcgag gcgctgtatg agtggaacta ccaacggcaa ctactcgcga ttcgacttgc
     4321 cgaagcgacg gcggagacca tcaccgacgc cgacctgttc accgaagaat tcttgctcga
     4381 aaggccgatc ctgcaggcag tgcgccatcg tgggccggcc gcaccggtgt tgctgatcga
     4441 cgagatcgac cgcgccgacg acgagttcga agcattgctg ttcgagttcc ttggggagtc
     4501 ttctgtgacg atcccggaac tcggaacgtt cactgctgtg cagccaccga tcgtggtgtt
     4561 gacgtcgaac cgcagccgcg aattgcacga cgcgttgcgg cgccgctgct tgtaccactg
     4621 gatcgaattc ccatcacccg aacgcgccgc cgagatcctt cgtcgcaccg ttccttcagc
     4681 aacggagttg ctgatccaga cgaccacgca gttcgtcggc cgtgtccgtg cgctcgaact
     4741 cgacaagccg cccggcatgg ccgagacgat cgattggctg tcggcactga gtgcgctcgg
     4801 tgcgaccgaa ctcctgcggg ctgacgtgtt actgacgctc ggtgccatcg ccaagacacc
     4861 tgacgaccgc gacacgatgg tcggtgcgtt agtcgagctt ggctactccg gcccgtcaaa
     4921 cccatgacca ttcaccgaca tgcaacgagg ttccgatgaa gatcgagaat gagttcagcg
     4981 tcaacgtgcc cgtcgacgag gcatggcagg tactcaccga cctggagcgc atcgcgccgt
     5041 gtatgccagg tgcgcagcta accggccggg aaggcgacgc ctttcttggc aaggtaaaag
     5101 tcaaagtcgg cccggtcatc agcgagttcg ccggcaccgc ttcgttcatc gagaagaacg
     5161 acgccaccca tcacgccgtc atcgatgcga agggccgcga ctcgcgtggc gcgggtaacg
     5221 cctcggcgat catcaccgca caactgcgag ccgacggcga tcaccgcacg gtggtgacgg
     5281 tcgagactga tctcaagatc agcggcaagc tcgcgcagtt cggcagcggc atgatcgccg
     5341 aggtgtcgag caagctgctc acccagttcg tcgactcact cgagaagcag gtcatgtcg
//
LOCUS       DASWVF010000754         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_276127, whole genome shotgun sequence.
ACCESSION   DASWVF010000754 DASWVF010000000
VERSION     DASWVF010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276127"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGD55_13310"
     CDS             complement(<1..189)
                     /locus_tag="VGD55_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871373.1"
                     /translation="MTDGLDAGGDDETLGSRSTSVSAWGAAGRVRFRPRGGILESMRW
                     WYRLSWVVAGFVVPLIAVY"
     gene            247..1452
                     /gene="serA"
                     /locus_tag="VGD55_13315"
     CDS             247..1452
                     /gene="serA"
                     /locus_tag="VGD55_13315"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0871374.1"
                     /translation="MSESRVLLLEQIHPDAEALLREAGYMVETNARALDEDELIGALA
                     GVQLLGIRSKTQVTARVLEAATDLEAIGAFCIGTNQIDLAAAARRGIAVFNAPFSNTR
                     SVVELALAEIIALTRHLTEKNALMHAGIWDKSAEGAHEVRGRTLGIVGYGNIGTQLSV
                     LAENLGMNVIYYDVVDRLSLSNARRCESIHELLAHADVVSLHVDGRHDNANLFGADEF
                     DAMKTGSLFLNLSRGFVVDYGSLRAHIESGHIAGAAIDVFPDEPSGRGDEFVSEVRGL
                     PNVILTPHIAGSTEEAQQDIGQFVATKLRDFLVEGSTAMSVNMPRITLRQNPGTHRML
                     HVHRNVPGVLARINGIFATHNANVEAQLLATTGEIGYVVTDIGSAFTTDMIAELEALP
                     ETVRFRSLS"
     gene            1501..>1866
                     /locus_tag="VGD55_13320"
     CDS             1501..>1866
                     /locus_tag="VGD55_13320"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0871375.1"
                     /translation="MPERPEPFISHATPAALDDLRVRLRATRWPDAPGDSGWSLGTDV
                     DYLRELVTYWANEFDWPAQEAALNRLPRYHMPIGDLGIHFVHARAATQPAMPLVLTHG
                     WPDSFWRYSKVVPLLTDPGA"
BASE COUNT          345 a          612 c          571 g          338 t
ORIGIN      
        1 gtaaaccgcg atcagcggca ccacgaagcc ggcgaccacc caagacaacc gataccacca
       61 acgcatggac tccagaatgc cccctcgcgg tcggaaccga actcgacccg ccgcccccca
      121 tgcgctgacc gatgtcgagc gcgatccgag cgtctcgtcg tcgccgcccg cgtcgagtcc
      181 gtccgtcacg ccccgatgct agggcgagct ggggtctgcc aatcaccggc acgagatact
      241 gaatacgtgt ctgagtcccg ggttctcttg cttgagcaga tccatcctga tgccgaggcg
      301 ctcttgcgcg aggccggcta catggtcgaa acgaacgcgc gcgcattgga tgaagacgaa
      361 ctcattggcg cgctggccgg cgtccaactg ctcggcattc gctcgaagac gcaggtcacc
      421 gcccgcgtcc tcgaagcagc gaccgatctg gaagcaatcg gcgcgttctg tattggtacg
      481 aaccagatcg atctcgcggc ggcggcgaga cgcggcatcg ccgtattcaa cgctccgttt
      541 tccaacacgc gtagcgtcgt cgaacttgca ctcgccgaga tcatcgcgtt gacgagacac
      601 ctcaccgaaa agaacgcgtt gatgcacgct ggtatttggg acaagtctgc cgagggagcg
      661 cacgaggtgc gcggccgaac gttgggcatc gtcggctacg gcaacatcgg cacgcagctg
      721 tcggtgctcg cggagaacct cggtatgaac gtcatctact atgacgtcgt cgatcggctt
      781 tcgttgtcga acgcgcgtcg ttgtgagtcg atacatgagt tgctggcaca cgcggacgtc
      841 gtgtcgttgc acgtcgatgg tcgacacgac aatgcgaacc ttttcggtgc agacgagttc
      901 gacgcgatga agacgggctc gctattcctc aacctttcgc gcggcttcgt cgtcgactac
      961 gggtcgctgc gcgcgcacat cgagtcgggt cacatcgccg gcgcagccat cgacgtgttt
     1021 cccgacgagc cgagcgggcg tggcgacgag ttcgtgtccg aggtcagagg gctgccgaac
     1081 gtgatcctga caccgcacat cgccggctcg accgaagagg cgcagcagga catcgggcag
     1141 ttcgtcgcca cgaagctgcg cgacttcctg gtcgaagggt cgacggccat gagcgtcaac
     1201 atgccgcgta tcacgctgcg ccaaaatccg ggcacgcatc ggatgcttca cgtgcaccgc
     1261 aacgttcccg gcgtcctggc gagaatcaac ggcatcttcg ccacgcacaa cgcaaacgtt
     1321 gaggcgcagc tgctggctac gaccggcgag atcggctatg tcgtgaccga catcggatcg
     1381 gccttcacga ctgacatgat cgcggagctt gaggctcttc ccgagactgt cagattccgc
     1441 tcgctgtcct gacgcttatt gccgcgacca ctgtcggtcg cgatcggtaa tgtgcgagac
     1501 atgccagagc gccccgagcc gttcatctcg cacgcaactc ccgctgcact tgacgatcta
     1561 cgcgtccggt tgcgcgcgac gcgctggccc gatgcgccag gcgattcggg ctggtcgctc
     1621 ggcaccgacg tcgattactt gcgcgagctc gttacctact gggcgaacga gttcgattgg
     1681 ccggcgcaag aggcggcact gaaccggcta ccccgctatc acatgccgat cggcgacctc
     1741 ggcatccact tcgtgcacgc gcgcgcggca acgcaacctg cgatgccgtt ggtcctgacc
     1801 cacgggtggc ccgactcgtt ctggcgctac tcgaaggtcg tgccgctact taccgatccg
     1861 ggcgcg
//
LOCUS       DASWVF010000755         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_199725, whole genome shotgun sequence.
ACCESSION   DASWVF010000755 DASWVF010000000
VERSION     DASWVF010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199725"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..363)
                     /locus_tag="VGD55_13325"
     CDS             complement(64..363)
                     /locus_tag="VGD55_13325"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY0871376.1"
                     /translation="MWLAIIAGSAGCFLLKLLGLSLPRKVVDQPRVKHTADLLPVALL
                     TALIATQTFTSGRHLQLDARAVGLVAAIVAVRFKAPFLVVVAVACVVTALVRLAT"
     gene            complement(363..1124)
                     /locus_tag="VGD55_13330"
     CDS             complement(363..1124)
                     /locus_tag="VGD55_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY0871377.1"
                     /translation="MADAGAASDASGPSDAAWTPIRTGILRDALGVGIATGAYGLSFG
                     AISTAAGLSVVQTCVLSLVMFTGGSQFAMVGVIGGGGAAMTGAATALLLGVRNSFYAV
                     RLTRLLDVHGFRRPVAAQLVIDESTAMAVVRMTPRAGRLAFWATGASVYVFWNLATLI
                     GALSAHAFSDPRVLGLDAAAPAAFLALMAPRLRSREPWVVALVAAAVALVSVPFVPAG
                     VPVLFAAAVAVVAGIRPGPPPVLEPSPSAALKDGF"
     gene            complement(1158..1850)
                     /locus_tag="VGD55_13335"
     CDS             complement(1158..1850)
                     /locus_tag="VGD55_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871378.1"
                     /translation="MADGDVVDLAETAVSGRRLGRHVEHDARSRNYPAAVAPIADVKH
                     MRHGKPFDQGDLGSCTGNAMAGALMTAPYYTASRVLTEPNAVSLYEQATHLDRVTGSY
                     PPDDTGSTGLAVAKAAKRDGYISAYSHAFGLQHALGALTLAPAIAGIHWYTSFDTPLP
                     TGECPLTAHATVRGGHEIELFGLDVEEQQIWAYQSWGPTWGGLGNGTFWFSYATFERL
                     LAEKGDVTAFTA"
     gene            1869..>2287
                     /locus_tag="VGD55_13340"
     CDS             1869..>2287
                     /locus_tag="VGD55_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871379.1"
                     /translation="MDFTDRMQQRPAVIQTVPVESSDFWRAVAMLRLERPAGLAALES
                     VFATGGVPTGLRGPMGGRFLASTLGHHLDPAFEALTAVWLPWKGKTFDADAEEGRNLF
                     TPGARRVMRVTLPTYQTRDEAGGRCSAFRFITSTGPSA"
BASE COUNT          413 a          785 c          775 g          314 t
ORIGIN      
        1 tagaacaacg cccagactcc acgatgatcg gagccgtggg tgatcggccg gcggggcgag
       61 gccctaggtc gcgaggcgca ccagcgcggt gacgacgcag gcgaccgcga cgaccacaag
      121 aaacggcgcc ttgaaccgca ccgccacgat cgcggccacc agtccgacgg cccgcgcgtc
      181 gagctggagg tggcgcccgg acgtgaacgt ctgggtcgcg atgagcgcgg tcagcagcgc
      241 gaccggcagc aagtcggcgg tgtgcttcac ccgcggttgg tcgacgacct tgcgcgggag
      301 cgacagaccg agcagcttca gcaggaagca accagccgat ccggcaatga tggcgagcca
      361 catcagaagc cgtccttcag cgcggcagaa ggtgacggct cgagcacagg cggcgggccc
      421 ggacggatgc cggccacgac agcaacggcc gccgcgaaca gcaccggcac gcccgccggc
      481 acgaacggca ccgacaccag cgcgacggct gccgcaacga gggcgacgac ccacggttca
      541 cgtgaacgca gccgcggcgc catcagcgcg aggaacgctg cgggagcggc cgcatcgagt
      601 ccgagcacgc gcgggtcgga gaacgcatgc gcacttagcg cgccgatcag cgtcgccagg
      661 ttccagaaca cgtacaccga cgcgccggtt gcccagaacg cgagtcgccc cgcacgcggc
      721 gtcatccgaa ctaccgccat tgcggtgctc tcgtcgatca ccaactgcgc cgcgaccgga
      781 cgccgaaagc cgtgcacatc gagcaaccgg gtgagccgca cggcatagaa gctgttgcgc
      841 accccaagaa ggagggcagt cgccgcaccc gtcatcgcgg cgccgccgcc cccgatcacg
      901 ccgaccatcg cgaactggct gccgccggta aacatcacca acgacaggac gcaggtttgc
      961 accaccgaaa gcccagccgc ggtcgagatg gcaccgaacg acaggccgta cgcaccagtc
     1021 gcaatcccga ccccgagagc gtcgcgcagg atgccagtgc ggatcggcgt ccaggccgca
     1081 tcggaagggc cggaagcgtc ggaagcagcg cccgcgtcag ccatgcggtc accatatggg
     1141 ggcgaccacc ggaaaagtca cgcagtaaat gcggtgacgt cgcccttctc ggcaagcagt
     1201 cgttcgaacg tcgcgtagga gaaccagaac gtgccgttgc ccaggccgcc ccaggtggga
     1261 ccccacgact ggtaggccca gatctgctgc tcctccacgt cgagcccgaa cagttcgatc
     1321 tcgtgtccgc cacgcacggt cgcgtgagcc gtcagcgggc actcgcccgt cggcagcgga
     1381 gtgtcgaagc tcgtgtacca gtggatgccc gcgatcgccg gcgcgagggt gagcgcgccg
     1441 agcgcatgct gcaagccgaa cgcgtggctg taagcggaga tgtagccgtc gcgcttcgcg
     1501 gccttcgcca cggcgaggcc cgttgatccg gtgtcatcgg gcgggtagct gcccgtcacg
     1561 cggtcgaggt gggtggcctg ctcgtacagc gacaccgcat ttggttcggt cagcacgcgg
     1621 ctcgcggtgt agtagggcgc ggtcatcaat gcgccggcca tcgcgttgcc ggtgcatgat
     1681 ccgaggtcgc cctggtcgaa aggcttcccg tgccgcatgt gcttcacgtc cgcgatcggc
     1741 gcaacggccg cagggtagtt gcgactgcgt gcatcgtgct cgacgtggcg acccaatcgg
     1801 cgtcccgata ctgctgtttc tgccagatca acgacatcgc cgtcagccat ggtgacctcc
     1861 tcattcaggt ggacttcacc gacaggatgc agcagcgacc ggccgtgata cagactgtgc
     1921 ccgtggaatc ctcggacttc tggcgcgccg tcgcgatgct gcgcctcgag cgcccggcgg
     1981 gtctcgccgc gctcgagtcg gtattcgcca caggtggcgt gccgaccggg ttgcgcggac
     2041 caatgggcgg ccgcttcctt gccagcacct tgggccatca cctcgatccg gccttcgagg
     2101 cgctcaccgc cgtgtggttg ccctggaagg gcaagacctt cgacgcggac gccgaggagg
     2161 gccgtaattt gttcactcca ggcgcccgca gggtcatgcg cgtgacgttg ccgacctacc
     2221 agacgcgcga cgaagcgggc ggacggtgca gtgcgttcag gttcatcacc tcgacgggtc
     2281 cgagtgc
//
LOCUS       DASWVF010000756        11755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_377904, whole genome shotgun sequence.
ACCESSION   DASWVF010000756 DASWVF010000000
VERSION     DASWVF010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377904"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VGD55_13345"
     CDS             <1..987
                     /locus_tag="VGD55_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871380.1"
                     /translation="TAAPAPRQTRVAVARLRDLALLPGIAVIMIVGTLSNKAFLTHGN
                     LIDVLDNFAWISMLVLAEAIILISGKLDLSLESTVALAPAIAVWLTLPKSGRGIALLP
                     GAWAIPITLAVGVLVGLINALLIVRFQLSSFIVTLGMLITLRGIETGISNGQTFFGFS
                     KSFLYLGTTHWFGLPLGVWLSVASFVVGIFVLSLTRPGRSLYAIGGNLNAARAAGIRV
                     ERTLWMVFILGSVLAAVGGLLYAGKYASIGATSGKGYIFTVFAATVLGGVSLNGGKGT
                     LFGAFCGIMLLYLVQNILTLAGVSGYWQDALNGAIILTVLIIARVTTGEAQA"
     gene            1078..1455
                     /locus_tag="VGD55_13350"
     CDS             1078..1455
                     /locus_tag="VGD55_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0871381.1"
                     /translation="MISAAMVVPTLPVTDLDKAKAFYRETLGLELLYENPFAARFRCG
                     DSSELSIFKRAPTLTEHTLAHFEVTDLDTLVADLEAKGVQFLDYTEGPLATTGHIARI
                     GPARGAWFHDPDGNTLGLREADA"
     gene            complement(1452..2456)
                     /locus_tag="VGD55_13355"
     CDS             complement(1452..2456)
                     /locus_tag="VGD55_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0871382.1"
                     /translation="MTKPPPATLSQVAKRAGVSLTTASKAINGKNRVSEETRARVMAA
                     AKDLAFTPNLVARGLITGRTSTVGFVIADSMTHRFAAPVMLGAEGALGASDLSMITCD
                     ARGDQERSRELALMLAARKVDGVLVVGDNNAVTASVTAHLDIPVVYVYGVSDDPADVV
                     HMPDDEKGMALAVDQLVVTGRLRIAHLTGPRGATAVVERVKGMNGRLRRDKLRLVRPV
                     QYGYWSQRWARSATVDLLAEHPDVDAVLCGSDQIASGVLVALKEAGRRVPDDVAVTGY
                     DNWGVFALETEPQLTTVDMNLEALGAAAAQELFSIIDGGPRHGGIRRHECALIVRGST
                     "
     gene            2894..4834
                     /locus_tag="VGD55_13360"
     CDS             2894..4834
                     /locus_tag="VGD55_13360"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY0871383.1"
                     /translation="MTDLVDVPDAVHIAVVGPVAPVTQTMRLRPLPLAGVSLGEAGWW
                     GQWQARNRATTLPYGIAALESAGNLDNLRRVIGESDAPYRGFVFSDTDVYKTLEAAAW
                     TLARHPDADLDAYIDAVAELLSRVQDADGYLNSNVQGTPGKLRFSHLAESHELYSAGH
                     FFQAAIADLRSTGNRRLLDVALRLADCLVAEFGANRRTDYDGHPEVETALVELFRVSG
                     NPAYLDLAKQFIDARGQQIFAADGRGSSYFQDEVPVREATSMVGHAVRALYLEAGVVD
                     VAVETDDALLLASSVKRWHDMVSSKLYITGGVGSRHKSEGFGDPYELPPDRAYCETCA
                     AIASIQWNWRLLLATGDAAYADLIERTIYNGFASSTGVDGTSFFYSNPLQVREEHEAS
                     DEEESGRRLPWYACACCPPNVMRLVATLQHYIASSDDAGVQIHQYTDADIDLRTTFRD
                     LVLALRTRYPWVGEVEVRIVRSASEPWTLSLRVPAWSADAQVRVNGELVAATARAGYI
                     ALKRSWAVGDLVTLELPMPARITAANPMVDAIRGCVAIERGPLVYCLEAIDNPGVDLA
                     AAALDIEHLLDVATIETLPDALAIRASGVMRAPVDVQPPLYMPAAHGEVMETQVDLIA
                     VPYYLWANRANGPMRVWLPRRS"
     gene            4940..5170
                     /locus_tag="VGD55_13365"
     CDS             4940..5170
                     /locus_tag="VGD55_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871384.1"
                     /translation="MGGYRLGDACSGAVEQCLLVEQAHGLDSVFYSPVGGIVGAATRG
                     DDVSSCDGKHSRARSVIVEWPRGDRHTVVLVS"
     gene            5115..6326
                     /locus_tag="VGD55_13370"
     CDS             5115..6326
                     /locus_tag="VGD55_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY0871385.1"
                     /translation="MSSSSGPAATGTLSFWSRDSDSFLTDIVAAYNASQSNIKVNLTL
                     VPQANFVSKLGSAVATHTGPDIIASDLVFAPYFAGAGAFKDITDFYNSLPYKSQFDPA
                     HVKQSTLNGKIYALPVTAEASVMYYNKTLFAQAKIAAPPTTYAEVLADANAITKLPGG
                     KDKGYYFSGACAGCNIFTFAPYIWASGGDVLDTNGQPTLNTPQVAAALKLYHDMWAAG
                     DIQASAKTDDGSFFSAPFANGNIGMVTSGAFFTASLVKPAAGKPTVDFGVVPIPGENG
                     GSSSFAGGDVIAIGSDTKNLAAAEAFMTWITSDGQKLIADKGDAVPIRSDIAASDYVP
                     KNPAIYTLLAAQLQTGKTPYSPVENALFNDGQGPWIQMIQKAVFGGDIAGAQAAAQTA
                     AAAIVAKGPGG"
     gene            6429..7388
                     /locus_tag="VGD55_13375"
     CDS             6429..7388
                     /locus_tag="VGD55_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0871386.1"
                     /translation="MAESSGVIAPALRTAGRRRTAMSRTPRRRGAGYRRESAGLVFAA
                     PALVMVAIFFIAPLCYMVWMAFQQYPLLGKHKFIGLQNFRDALKDTTFTHAVGLTIEY
                     TALVVPLLLIVGLGLASLVSSRRRGAAFFRTIFFLPVVVGLASASYLWYWLVDAGVGP
                     LPYILRHLGFGQLSNPWLGGMPGALFVVTLMIVWKVSGLTMLLLMTGLQSIPVDIVEA
                     ARMDGAGRVRRLRSITLPLLRNTIALVLILTTAGSFLAFDQFYVMTKGGPANQTLTIA
                     YWIYHVSFVNYRLGYGAALSVMLALVLLVISALQLRILNDSRE"
     gene            7390..8280
                     /locus_tag="VGD55_13380"
     CDS             7390..8280
                     /locus_tag="VGD55_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0871387.1"
                     /translation="MTVVTESVEVVAEPERLTTKSRFSLPALAWWTICICIAILFALP
                     LLLGLVSSLRPPAVSQGSPELFPKQISFGNFHGLNKLGSGIGSYLRNSVLVACATVIG
                     TVVLATLAGFGFAKFRFFGRRIAFLAIILMLMIPFQAIVTPLYVMLVKVKLSDSLIGL
                     AAVYITYQLPFSIFVMRNAFSAVPDALQESAHMDGCSTWGGFTKIMLPLARPGAITVA
                     LFAFFTAWNEFFAALIFLSNQGKYTLPIMLTLIQGQQYGQVDWGLLQAGVMVTMLPCI
                     VIFITLQRYYVQGMLSGSVK"
     gene            8328..9311
                     /locus_tag="VGD55_13385"
     CDS             8328..9311
                     /locus_tag="VGD55_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0871388.1"
                     /translation="MDVAVLAGVSHQTVSRVVNRHPGVKATTRIRVQAAIEELGYRRN
                     HAARALATGRTQTLGVVMLNSTLYGPASTLHGIEQAALDSQYFITVASLQSPDRLALQ
                     DAVSRLVDQGVEGVLVIAPLVSLVEALGELPTGLPMVVIEGDTHAEVDVVTVDQVAGA
                     KAATRHLLELGHPTVWHVAGPSDWLEAQQRTAGWRAALEEAGADVPPPLFGDWSPRSA
                     FAAGRALARIPELSAVFVANDSMALGVLRALHDAGRRVPDDVSVVGFDDTPEAAYFPA
                     PLTTVRQDFGEVGRRGLALLIDQMQSGPRPPRRVLVQSTLIVRKSTSEPPG"
     gene            complement(9312..10346)
                     /locus_tag="VGD55_13390"
     CDS             complement(9312..10346)
                     /locus_tag="VGD55_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871389.1"
                     /translation="MTTQTTKHTALTPSEIDPAPKNLVARMLRKLAESPEAGVVTACV
                     LVFIAISLKAPTYHNAGNLQVMGRDLSQVGILAIGEALVILTGGIDLSVGGLAGLAGI
                     LTAWFNVREGLSAPVAIAVTLVICAAIGFWHGTMVTKLQVAPFVITLVTLTVSEGLAL
                     AITTGTPINGISSLFSKMSRFGVFDVPLPAVIFAGVVAITWVILERTYVGRQIYAVGG
                     NREAARLAGIPTARRIISCYVASAALAGVVGVLVIGRMNVADPSVGSTGWELVAIAAA
                     VVGGVALTGGEGRILGVAAGAILLEFITNGLLAMRVSPYYQQVVQGAVLGIAILADRF
                     RARKFGRTRA"
     gene            complement(10343..>11755)
                     /locus_tag="VGD55_13395"
     CDS             complement(10343..>11755)
                     /locus_tag="VGD55_13395"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0871390.1"
                     /translation="GTLSGSVFRDGGDVEVGGTPLVPGSTEAASRLGVALVSQEFPLV
                     GQLSVAENMLLGRRPRESKRRFFIDRRAMRAEAEAMLTEIGLSPKAIPVNRKVRSLPV
                     PTRQMIEIAKAWGREPTLLILDEPTSSLGPVEAQIVLDLARQLAERGGAVLFIGHRLD
                     AVRRVSDRIIVLRNGRLVADLQPNEASEDRLIREMVGGELAHSEPKTKPQQAKVVLSI
                     EALTADGLGPVDLELHAGEVLGVAGLMGSGRSRLIHTVAGAQPATGGSMELAGRLYTP
                     HNAGEGTAVGIAMIPEDRKEQALVPFQPIRSNVTLSVVKRLSSRGFLRPKRIRTEAVA
                     IAGSVNVRMQSVDQPISSLSGGNQQRAIFGRAFATKPRVLLLDEPTRGVDVGAKAEIY
                     ELINQAANDGMAVLVASSELEELLWMCHRIAVMNHGVIVDVIDRENATKERIMKAAAG
                     TLTGAVQPKPPDPGMDGAVA"
BASE COUNT         2024 a         3830 c         3665 g         2236 t
ORIGIN      
        1 acggctgcac ccgcgccccg gcagacgcgc gtcgcggtgg cccgcttgcg cgacctcgcg
       61 ctgcttcctg gcatcgccgt catcatgatt gtcggcaccc tcagcaacaa ggcgttcctc
      121 acccacggca acctgatcga cgtcctcgac aacttcgcct ggatatcgat gcttgtgctg
      181 gccgaggcga tcatcctcat cagcggcaag ctcgacctgt cgctcgagtc gaccgtggcg
      241 cttgcgcccg cgatcgcagt atggctgacg ttgccgaagt cggggcgtgg catcgcgttg
      301 ctgccgggtg catgggcgat cccgatcacg ctcgccgtcg gcgtccttgt tggactcatc
      361 aacgcgttgc tcatcgtgcg cttccagctc agctcgttca tcgtgacgct tggcatgctc
      421 atcacgctgc gcggtatcga gaccggcatc tcgaacggcc agacattctt cggtttcagc
      481 aagtcgtttc tctatctcgg cacgacgcac tggttcggac taccgctggg tgtgtggttg
      541 tcggttgcgt cgttcgtggt cggcatcttc gtgctgtcgc tcacccggcc gggccgttcg
      601 ctctatgcga ttggcggcaa cctcaacgcc gcccgagcgg ctggcatcag ggtcgaacgc
      661 acgctgtgga tggtgttcat ccttggcagc gtgctggccg cggtcggcgg tctgctctat
      721 gccggtaagt acgcctcgat cggggctacc agcggcaagg gatacatctt caccgtcttc
      781 gcggcgaccg tgctcggtgg cgtgagcctc aacgggggga agggcacgct gttcggtgcc
      841 ttctgcggca tcatgctgct ctacctcgtg cagaacatcc tgaccctggc cggggtgagc
      901 ggctactggc aggacgctct caacggcgcg atcatcctga ccgtgctgat catcgctcga
      961 gtcaccacgg gcgaggcgca ggcttgacgt agcacgagcc tggtcaccgg tctgtcttgt
     1021 gcgtagtcgc gcgcaggtgt acacagcgac gacgggagaa tcgactggag gcaaccgatg
     1081 atcagcgcag ccatggtcgt gccaacgctg cccgtgaccg atctcgacaa agcgaaagcg
     1141 ttctaccgcg agaccctcgg cttagagttg ctttacgaga acccgttcgc tgccaggttc
     1201 cgttgcggcg acagcagcga actctcgatc ttcaagcgcg caccgaccct gacggagcac
     1261 acgctcgcgc attttgaggt gaccgacctt gacacgctcg tggcggacct cgaggcgaag
     1321 ggcgtccaat tcctcgacta caccgaaggc ccgctcgcca cgaccggcca catagcgcgg
     1381 atcggacccg ctcgcggtgc gtggttccac gaccctgacg gcaacacact tggtctgcgc
     1441 gaagccgacg cctaagtaga accgcggacg atcagcgcac attcgtggcg gcggatgccg
     1501 ccgtgccgtg ggccgccgtc gatgatgctg aacaactctt gcgccgcggc ggctccgagc
     1561 gcttctaggt tcatgtcaac agtcgtgagt tggggttcgg tctcgagcgc gaacacgccc
     1621 cagttgtcgt agccggtcac ggctacatcg tcagggaccc gcctaccggc ttcctttaac
     1681 gcaaccagga cacccgacgc gatctgatcg gagccgcaca ggaccgcatc gacgtcgggg
     1741 tgctcagcca gcaggtcgac cgtcgccgat ctcgcccagc gttgcgacca gtagccgtac
     1801 tgcaccggcc gtacgagccg gagtttgtcg cgtcgaagcc ggccgttcat gcctttcacc
     1861 cgctcgacga cggcggtcgc cccgcgtggg ccggtgaggt gtgcgatccg gaggcgtccg
     1921 gtcaccacga gttggtcgac ggccagcgcc atgcctttct cgtcgtcggg catatggacg
     1981 acgtctgccg ggtcgtcgga caccccgtat acgtagacga cgggaatgtc gagatgtgcg
     2041 gtgaccgacg cggtcaccgc gttgttgtca cccacgacga gcacgccgtc aaccttgcgt
     2101 gccgccagca tgagcgcgag ctctcggctg cgctcctgat cgccccgtgc atcgcaggta
     2161 atcatcgaga ggtcgctcgc gccgagcgcg ccctcggcgc cgagcatgac cggcgccgcg
     2221 aagcggtggg tcatgctgtc ggcgatgacg aacccgacgg tgcttgtgcg acccgtgatc
     2281 agacctcggg ctacgagatt cggcgtgaac gcgaggtcct tcgccgccgc catcacccgc
     2341 gctcgggtct cctccgacac ccggttcttg ccgttgatcg ccttcgaggc ggtcgtcaac
     2401 gagacgccgg cgcgcttagc tacctggctg agggtcgccg gcggcggctt cgtcatggcg
     2461 cgagtgtgcc atgaccctgc cgagatagac ggctacaggg tcgcgcctcc agtgcttgcg
     2521 accgcctccg catgaaggtg aacaggatcg attgctgccg gctgaggaag cgcttcctca
     2581 accggcgact catcctgagc cctgtcggcg cagggcgtca aggccccttt gaagaccatc
     2641 aagaatgcct cagataaacc cgaacggatc gatacacgct cgcaaataag acatctgttc
     2701 cggccgttcg taacaagttg gtaactccga cggcaccggg cttgacgcgc agtcggccga
     2761 ggcccaacct gtgcacacca gtgaggaagc gcttcctcat cacatcgtgt cgggctctgt
     2821 agccggcccg ccgggtgacc tttcctgtgc cacacggcga tgacgcatgc cctccaaacg
     2881 ctaggagccc cctttgaccg acctggtcga cgtgcccgac gcggtccata ttgccgtcgt
     2941 cggacccgtc gctcccgtga cgcagacgat gcgcttgcgg ccgctgccgt tggctggagt
     3001 ctcgttgggc gaggctggtt ggtgggggca gtggcaagcg cgcaatcggg ccactacgtt
     3061 gccgtacggg atcgcggcgt tggagtcggc gggcaacctc gacaacctcc gtcgagtcat
     3121 cggtgaaagc gatgcgccgt accgcggctt tgttttcagc gacaccgacg tgtacaagac
     3181 gctcgaggct gcggcttgga cgctcgcacg tcatccggac gcagatctcg acgcctatat
     3241 cgatgctgtc gcagagttgc tctccagagt tcaagacgcc gacggctatc taaacagcaa
     3301 cgtccaggga acacccggca agctgcgctt ctcccacctc gccgagtcgc acgagctgta
     3361 ttccgccggg cacttctttc aagctgcgat cgctgacctt cggtctaccg gcaaccgcag
     3421 actcttggac gtcgcacttc ggcttgccga ttgtttggtc gcagaattcg gcgccaaccg
     3481 tcgcactgac tacgacggtc atcccgaagt cgagaccgca ctggtcgaac tcttccgggt
     3541 aagtggcaac ccggcgtacc tcgatcttgc aaagcaattc atcgacgcac ggggtcaaca
     3601 aatcttcgcg gccgacggcc ggggcagctc ctacttccag gacgaggtgc ccgtccgtga
     3661 ggccacgtcg atggtcggac acgccgtcag ggcgctctac ctcgaagcgg gcgtcgtcga
     3721 tgtcgctgtc gagaccgacg atgcattgct cctcgcgtcg tcggtaaagc ggtggcacga
     3781 catggtttcg agcaagctct acatcaccgg cggagtcggg tcgcggcaca agagcgaagg
     3841 gtttggggac ccgtacgagc tgccgccgga tcgcgcctat tgcgaaacgt gtgcggcgat
     3901 agcgagtatc cagtggaact ggcgcctttt gctcgcgacc ggcgatgctg cgtacgccga
     3961 cttgatcgag cgaactatct acaacggatt cgcttcgagt accggcgttg acggcacgtc
     4021 cttcttctac tcaaatccgc tccaagtgcg ggaggagcac gaagcctccg acgaggaaga
     4081 gtcaggacgt cgcctgccgt ggtacgcctg cgcgtgctgt ccaccgaacg tcatgcgctt
     4141 ggtcgcaacg cttcagcact acatcgcgtc gtccgatgac gccggcgtcc agatccacca
     4201 gtacaccgac gccgacatag acctgcgcac caccttccgc gatctggttc tggcgctgcg
     4261 cacccgttat ccatgggtcg gcgaggttga ggtgcggatc gtgcgatccg caagcgaacc
     4321 ttggacactc tcgctccgag ttccggcctg gtctgccgac gcgcaagtcc gcgtcaacgg
     4381 cgagctcgtt gccgcgacag ctcgtgcggg ttacatcgca ctcaagcgca gctgggccgt
     4441 cggcgacctc gtcactctcg agttgccaat gcccgcccgc attaccgccg ccaacccgat
     4501 ggtcgacgcg atccgcggct gcgttgcgat cgagcgcggt ccgctggtgt attgcctcga
     4561 ggcgatcgac aacccaggag tcgacctcgc tgcggccgcg ctcgacatcg agcacctgct
     4621 cgacgtggca acaatcgaga ccttgccgga cgcgttggcg atccgagcga gtggcgtgat
     4681 gcgcgcgccc gtcgacgtcc agccgccttt gtacatgccg gctgcccatg gggaggtgat
     4741 ggagacgcag gtcgacctca tcgccgttcc gtactaccta tgggccaacc gagccaacgg
     4801 accaatgcgc gtttggctcc ccaggcgcag ctagccgcgt cgcgatccgc ctccaaccgc
     4861 gcgctcctca gaacttcgac cgcaccgacc caccggaatt ctatggaaag gtcagacacg
     4921 atatgaacgc caagcaacga tgggcgggta tcgcctcggc gacgcttgct ctggcgctgt
     4981 tgagcagtgc ttgctcgtcg agcaagcaca cggcctcgac agcgtcttct actcccccgt
     5041 cggcggcatc gtcggcgccg caacccgcgg cgacgacgtc agctcctgcg acggcaagca
     5101 ctcccgcgcc cgcagtgtca tcgtcgagtg gccccgcggc gaccggcaca ctgtcgttct
     5161 ggtctcgtga cagtgactcg ttcctcaccg acattgttgc ggcttacaac gcgagccaga
     5221 gcaatatcaa ggtgaacctg accctcgtgc cgcaggcgaa cttcgtctcg aagctcgggt
     5281 cggcggtggc gactcacaca ggccctgaca tcatcgcgag cgacctggtg ttcgcgccct
     5341 actttgcggg cgcaggtgcc ttcaaagaca tcaccgactt ctacaactcg ctgccgtaca
     5401 agtcgcagtt cgatccggcg cacgttaagc agtcgacgct caacggcaag atctacgccc
     5461 tgcccgtaac cgccgaggcg tcggtcatgt actacaacaa gacgctgttc gcacaggcga
     5521 agatcgcagc cccgccgacc acctatgcag aggtgctggc cgacgccaac gccatcacga
     5581 agctgcccgg cggtaaggac aagggttact acttctccgg tgcttgcgct ggctgcaaca
     5641 tcttcacctt tgctccctac atctgggcaa gcggtggcga cgtgctcgac accaatggcc
     5701 agccaactct caacaccccg caggtggcgg cagcgctgaa gctgtaccac gacatgtggg
     5761 ctgccggtga cattcaggcc tcggcgaaga ccgacgatgg ctccttcttc tccgcaccat
     5821 ttgccaacgg caacatcggc atggtcacct ccggtgcgtt cttcaccgct tcactggtga
     5881 agccagcggc cggtaagccg acagtcgact tcggggtggt tccgatccct ggtgagaacg
     5941 gtggatcctc ttcgttcgcg ggtggcgacg tgatcgcgat cggaagcgat acgaagaacc
     6001 ttgcagccgc tgaggcgttc atgacttgga ttacctctga cggccagaag ctcatcgccg
     6061 acaagggcga cgccgttccg atccgttcag acatcgcggc tagtgactac gtgccgaaga
     6121 acccggcgat ctacacgctg ctggccgcgc agctccagac cggcaagacc ccgtacagcc
     6181 ccgttgagaa cgcgctcttc aacgacggtc aggggccgtg gatccaaatg atccagaagg
     6241 cagtgttcgg tggcgacatc gccggcgcgc aggcagctgc gcaaacggcc gccgcagcga
     6301 tcgtggcgaa gggcccgggc ggctgatcaa tcgccgtcca tctacaaccg gggacggcgg
     6361 gatctcctcg ccgtccccgg tgctcggcat cgaagcgcgc gacttaggct gaggcgacag
     6421 gagtaacgat ggcggagtca tccggagtca tcgccccggc actgcgcacc gccggtaggc
     6481 gccgcacggc aatgagccgt actccgcgtc gtcgtggcgc aggttatcgt cgcgaaagcg
     6541 caggcctcgt ctttgctgca ccagcgctcg tcatggtcgc catcttcttt atcgcacccc
     6601 tgtgttacat ggtctggatg gccttccagc aatacccgct gctcggcaag cacaagttca
     6661 tcggtttgca aaactttcgc gatgcgttaa aggacaccac cttcacgcac gcggtcggtc
     6721 tcacaatcga gtacacagcg ctcgttgttc cgctgctgct gatcgtgggt cttgggcttg
     6781 caagtttggt gagcagtcga cgccgcggcg cggcgttctt ccggacgatc ttcttccttc
     6841 ctgtcgtcgt gggtctcgcg tcggctagct acctctggta ctggctggtc gatgcggggg
     6901 tcggtccgct cccgtacatc cttcgccatc tcggcttcgg gcagctgagc aatccctggc
     6961 tcggcggcat gccaggggca ctgttcgtcg tcactttgat gatcgtgtgg aaggtgagtg
     7021 gcttgacgat gctgctgctc atgacggggt tgcagtcgat tccggtcgat atcgtcgaag
     7081 ccgcacgcat ggacggcgcg gggcgggtgc gtcggctacg cagcattact ctcccgctgc
     7141 tgagaaacac gatcgccctc gtgctgatcc tgacgaccgc cggctcgttc ttggcgttcg
     7201 accagttcta cgtcatgacc aagggcggcc cggccaacca gacgctcacg atcgcgtact
     7261 ggatctacca cgtctcgttc gtgaactacc ggcttggtta cggtgcagct ctgtcggtca
     7321 tgttggcact cgtactcctc gtcatcagcg cgctgcaact gcgcatcttg aacgactcgc
     7381 gggagtgaca tgaccgtcgt cactgaaagc gtagaagtag tagcggagcc ggaacggcta
     7441 acgaccaagt cgcggttctc gttgcccgcg ttggcctggt ggacgatctg catctgcatc
     7501 gcgattctgt tcgcgttgcc gctccttctc ggtctcgtgt catcgctgcg tcctcctgcg
     7561 gtatcgcaag gctcgccgga gttgtttccg aagcagatct cgttcggcaa cttccacgga
     7621 ctcaacaagc tcggctcggg catcggctcg tatttgcgca acagcgtgct ggtcgcttgc
     7681 gcgacggtga ttggaaccgt cgtgctcgcc accctcgcgg gatttgggtt cgcgaagttc
     7741 aggttcttcg ggcgccggat tgcgttcctg gccatcatct tgatgctgat gattccgttc
     7801 caggcgatcg tgaccccgct gtatgtgatg ctcgtcaagg tcaagctttc cgacagcctc
     7861 atcggcttgg cggctgtcta catcacctac cagttgccgt tctcgatctt tgtgatgcgc
     7921 aacgcgttct ccgccgtgcc cgacgcgctt caagagagcg cccacatgga cggctgtagc
     7981 acctggggag gattcaccaa gatcatgctc ccgctggcca ggccaggggc gatcaccgtc
     8041 gcgttgttcg cgttcttcac ggcgtggaac gagttttttg ccgcgctgat tttcctttcg
     8101 aatcaaggta agtacacgct accgatcatg ctgacactga tacagggtca gcagtacggc
     8161 caggtcgact ggggtctgct tcaggccggc gtcatggtga cgatgctgcc ctgcatcgtc
     8221 atcttcatca ccttgcagcg ttactacgtc cagggcatgt tgagtggctc ggtcaagtag
     8281 atgagcgtgg ttgggtgtca gtgctaggta ccggtcggcc cgggatgatg gacgtcgcgg
     8341 tgttagcggg agtttcacat caaacggtgt cgcgagtcgt caatcgccac cctggggtga
     8401 aagcgacaac acgcatcagg gtgcaggcgg cgatcgagga gctcgggtat cgccgcaatc
     8461 acgctgcccg cgcattggcg accggccgca cccagacact gggtgttgtg atgctcaaca
     8521 gcaccttgta cggacccgcg tcgaccttgc acggcatcga acaagctgcg ctcgactcgc
     8581 agtacttcat caccgtcgcg agtttgcagt cacccgatcg ccttgcgctg caagacgcgg
     8641 tcagccggct ggtcgatcag ggcgtcgaag gggtgctggt gatagcgccg ctggtgtcgc
     8701 tcgtcgaggc gttgggggag ttgcccaccg gattgccaat ggtcgttatc gaaggcgaca
     8761 cccacgccga ggtcgacgtc gtaacggtcg atcaggtggc cggcgcgaaa gctgcgaccc
     8821 gccatctgct cgagctcggg cacccgacgg tgtggcatgt cgcaggtcca agcgactggc
     8881 tagaagcaca gcagcggacg gcggggtggc gggccgcgct cgaagaggcc ggcgccgacg
     8941 ttccgccacc gcttttcggc gactggagtc ctcggtcggc gtttgctgcg ggtcgcgcac
     9001 tagctcggat ccctgagctg agcgcggttt ttgtggcgaa cgattcgatg gcgctagggg
     9061 ttctgcgcgc cttgcacgac gctggtcggc gggtgcccga cgacgtaagc gttgtcgggt
     9121 tcgacgacac ccctgaagcc gcctacttcc cggcaccact gaccacagtg cgacaagact
     9181 tcggcgaggt cgggcggcgc gggctggccc tgctgatcga ccagatgcaa tcgggaccgc
     9241 gaccgcctcg acgggtgttg gtccagagca cgctcatcgt tcgcaagagc accagcgaac
     9301 cacccggtta gctacgcccg cgtgcggccg aacttgcgag cacggaaccg gtcagccagg
     9361 atcgcgatgc cgagcactgc gccctgcacg acctgctggt aatacgggct tactcgcatc
     9421 gctagcagac cgttggtgat gaactcgagc aggatcgcgc ccgcagcgac accaaggatc
     9481 cggccctcac caccggtgag cgctacgcca ccgacgaccg cggccgcgat cgcaaccaac
     9541 tcccagccgg tgctgccgac cgacgggtcg gcgacgttca tccggccgat gaccagcaca
     9601 ccgacgacac cggcgagcgc ggcactcgcg acgtaacacg agatgatgcg tcgcgcggtc
     9661 ggaatgccgg ccagtcgcgc ggcttcgcgg ttgccgccga cggcatagat ctggcgcccg
     9721 acataggtgc gttcaaggat cacccaggtg atcgcgacta ctccggcgaa gatcactgcg
     9781 ggtaagggca catcgaacac cccgaaacgg ctcatcttgc tgaagagcga ggagatgccg
     9841 ttgatcggcg tcccagtcgt gatcgcgagc gcgaggcctt cggaaaccgt aagcgtcacg
     9901 agcgtgatga cgaacggcgc gacttgcagt ttcgtcacca tggtgccgtg ccagaacccg
     9961 attgccgcgc agatcaccaa cgtcacggcg atcgcgactg gtgctgacag cccttcgcgc
    10021 acgttgaacc aagcggtcaa gatgccggcg agaccggcga gcccaccaac cgataggtcg
    10081 atgccgccgg tgaggatgac gagcgcttcg ccaatggcaa ggatgccgac ctggctgagg
    10141 tccctgccca tgacctggag gttcccggcg ttgtggtagg tcggcgcctt cagcgagatg
    10201 gcgatgaaca ccagcacaca ggcggtgacg accccagctt caggggactc cgcaagtttg
    10261 cggagcatgc gcgccacaag attcttgggc gccgggtcga tctcacttgg ggtgagggcg
    10321 gtgtgcttcg tggtttgcgt ggtcacgcta ctgctccgtc catcccaggg tcaggcggct
    10381 tcggctgcac agctccggta agcgtcccgg cagcagcctt catgatgcgc tctttggtgg
    10441 cgttctcgcg atcgatcacg tcgacaatca caccgtggtt catcacggca atccgatgac
    10501 acatccacag caactcttcg agctcggagg aggcaaccaa cacggccata ccgtcgtttg
    10561 cggcttggtt gatcagctcg tagatctccg ctttggcgcc gacgtcgacg ccgcgagtcg
    10621 gttcgtcgag caacaagacg cgtggcttcg ttgcgaacgc ccgtccgaag atcgcccgtt
    10681 gctggttccc gcccgacagc gaactgatgg gctggtcgac cgactgcatc cggacgttca
    10741 cactccccgc gatagctact gcctcggttc gaatgcgctt cgggcgcaga aatccccgcg
    10801 aactaagccg cttgaccacc gagagcgtga cgttcgatcg gatcggttga aacggcacga
    10861 gcgcttgctc tttgcggtct tcaggaatca tcgcgatgcc gaccgcagta ccttcgcctg
    10921 cgttgtgcgg ggtgtagagc cgccctgcca actccatcga cccgccggtc gccggctgag
    10981 cgccggccac cgtgtggatc agcctgctgc gtccggaacc catgagcccg gcgactccga
    11041 gaacctcgcc ggcgtgcagc tcaaggtcga ccgggccgag tccgtcggcg gtcagggctt
    11101 cgatgctcaa cacgaccttt gcctgctgcg gcttggtctt tggctcgcta tgtgcgagct
    11161 cgccgccgac catctcgcgg atcagccggt cttcggacgc ctcgttcggt tgtaggtcag
    11221 cgaccagtct tccgttgcgc aacacgatga tgcggtcgct gacgcggcgc accgcgtcga
    11281 ggcggtgacc gatgaagagg acggcgccgc cgcgttcggc aagctgcctg gcgaggtcca
    11341 acacgatctg cgcctcgaca gggccgagcg atgacgtcgg ttcgtcgagg atcagcagcg
    11401 ttggttcacg gccccacgct ttggcgatct cgatcatctg gcgcgtgggc accggcaggc
    11461 tccgtacctt gcgattcacg gggatcgcct tcggcgagag gccaatctcc gtgagcatcg
    11521 cttcggcctc agcccgcatt gcgcgccggt cgatgaagaa ccgccgcttc gactcccgcg
    11581 gacgtcggcc gagcagcatg ttctccgcga ccgaaagctg tccgacgagc ggaaactcct
    11641 gcgacacgag ggcgacgccc agccgtgacg ccgcttcggt cgaccccggg acgagcggtg
    11701 tcccgcctac ctcaacgtcc ccgccgtccc ggaaaactga cccggaaagc gtgcc
//
LOCUS       DASWVF010000757         4811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_250683, whole genome shotgun sequence.
ACCESSION   DASWVF010000757 DASWVF010000000
VERSION     DASWVF010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250683"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /locus_tag="VGD55_13400"
     CDS             <1..2208
                     /locus_tag="VGD55_13400"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY0871391.1"
                     /translation="LAMALVWAALVTALYAHTVAPLGFIPGPAALRQLHVTMSSGFTD
                     TVQLAAPVEVTRGLSLLTTAGVGLVAVVVETLASALRRPAVAGLPLLAIFTVSAATIS
                     KGVGWIPFVFAAAGYLALLLAEGRDRVSRWGRPVRAGRGRKPSARGQTVTTQLTQVGR
                     RVGFAAISIAVVVPVIVPGLHSGWFGSHHTNGVGGFTSNPNEGSASISPIVSIGQELN
                     EPAPNSLFTYTTTGTPTYLRLATLDRFDGTQWTVSTVSTPTDARISKGIPAPAGVTLQ
                     PTGSFDTDVTITGLRQAYLPVPTTATAVQARGDWRYNPVNPAVYGVNTSTLHLDYSAT
                     SAVYQPSPTYLNSLAAVAANESIAPYLALPAGLPPQVHQIADEIVANAGAVSQYQKAI
                     ALQAWFQAGTYDISVKTGTSETALLTFLSNRRGFCEQFAATMALMARLEGIPARVDVG
                     FTPGTKVANSNSYLVTTADAHAWPELYFPGAGWLRFEPTPRSDGQTSDPSYANAAVGP
                     QPAASGTAVPNDAVAKPQIPNFHTGGTPAASKSRHHGFALGKLSAGWLTAVLVVLLGL
                     VVAPLTRWTTRRRRWTAADTEAARAHAAWDELGDDVRDLGLEWGGDSDTPRRAAATLV
                     ATRRLAYDVAAQQALSRLTRAEELARYAARPDELRWVDQDPRADEKAVRKALFTSVSR
                     LRRLRARVAPPSTGRLAANATAWIGDTWHGFTAGSGRWVGRQFNRRADRDGQD"
     gene            complement(2254..2724)
                     /locus_tag="VGD55_13405"
     CDS             complement(2254..2724)
                     /locus_tag="VGD55_13405"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY0871392.1"
                     /translation="MPLSEHEQRVLEQIERALYADDPKFAATVRGIDPRTLHRRRYLR
                     AAIGGIVGVVLLPVGIAVQQLELSVVGFLLMLLAFLYGASTWRQTREVPDAAAAPGAG
                     VVRPAEFTQARRRRGEKPVGRPVVKRGLVQRFEERWDRRRDSLGGRDDSDGRPD"
     gene            complement(3019..3774)
                     /locus_tag="VGD55_13410"
     CDS             complement(3019..3774)
                     /locus_tag="VGD55_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0871393.1"
                     /translation="MADRLRTRGSVRTAVVLRSVRSLLVDMVAETGRVHLDVLDAGGG
                     TGGFAVPLAELGHHVTVVDASPDSLAALDRRAADAGVTDRVRSVQGDVASLLDVVRPA
                     SFDLVVLHSVLEVVDDPTAVLTAVTAVLRPGGVTSVVAANRLAAVLHRAAAGRFDEAL
                     HALRDPVGRYSDTDPAPRRFALADLEALLAMAGLTVVGTHGSRVFADLVPGGSLDADP
                     AAVETLIALEFEASEIPAFREIATQLHVVGRRG"
     gene            complement(3792..4163)
                     /locus_tag="VGD55_13415"
     CDS             complement(3792..4163)
                     /locus_tag="VGD55_13415"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0871394.1"
                     /translation="MRGRLHHVIFDCPDPGAIATFYSTLLNQPITYASADFVVVAANE
                     TTSGIAFQRAPDHRAPTWPDSAVPQQAHLDIMVESVADAGPWVLSLGAIKLDGDNVFA
                     DPAGHPFCLIQRPGWATPIAD"
     gene            complement(4201..>4811)
                     /locus_tag="VGD55_13420"
     CDS             complement(4201..>4811)
                     /locus_tag="VGD55_13420"
                     /inference="COORDINATES: protein motif:HMM:NF037499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HEY0871395.1"
                     /translation="LAPGPRSSTHRGSGASSGQLLLLLAAAAGANYRAARKEAGDMTM
                     TSMSSTLERVQPVQRVQRANLPAALGLLASARHGLDEAATSSRPAQRYASAHLAALRA
                     AAAVLAARARPEIPGVRRVRRSRNAWVLLTQVAPELAEWAALFAAGAGKRAAAEAGLD
                     GAVTARESDDLVRDAEIFLALVETTMGVIHQPTLPVDTIRAS"
BASE COUNT          843 a         1660 c         1583 g          725 t
ORIGIN      
        1 cttgcgatgg cgcttgtgtg ggctgcgctc gtgacggcgc tctacgcgca cacggtcgcg
       61 ccgctcggct tcattcccgg ccctgccgca ctgcgccaac tccacgtcac gatgtcgtcg
      121 ggcttcaccg acaccgtgca actcgccgcc cccgtcgagg tcactcgcgg tctcagcctg
      181 ctgacgaccg ccggggtcgg actcgtcgcc gttgtcgtcg agaccctcgc cagtgcactt
      241 cggcgtccgg ccgtcgcagg tttgcctctc ctcgcgatct tcaccgtctc ggccgcgacg
      301 atttccaagg gtgtcggctg gattccgttc gtcttcgcag ccgccggtta cctcgccctg
      361 ctgctcgccg aagggcgcga ccgggtcagt cgctggggcc gacccgttcg ggcgggccgc
      421 ggtcgcaagc catccgcacg cgggcaaacg gtgacgacac agttgaccca ggtcggacgc
      481 cgggttggtt tcgcggcgat cagcatcgcc gtcgtcgtcc cggtcatcgt tcctggcttg
      541 cattcgggtt ggttcggcag ccaccacacc aatggagtgg gcggtttcac ttcaaatccg
      601 aacgaagggt cggcgagcat cagcccgatc gtgagcatcg gccaggagct gaacgaaccg
      661 gcgccgaact cgcttttcac ctatacgacg acgggaacgc ccacgtatct gcggctcgcg
      721 accctcgacc gcttcgacgg cacgcagtgg accgtgtcta ctgtcagtac accgaccgat
      781 gccaggattt ccaagggaat tccagcgcct gccggcgtca ccctccagcc gacgggcagc
      841 ttcgacaccg acgtcacgat caccgggctt cggcaggcct acctgcccgt accgacgaca
      901 gcaaccgcgg tgcaggctcg gggtgattgg cgctacaacc cggtgaaccc cgccgtctac
      961 ggtgtcaaca cctcgacgct gcacctcgac tactccgcga ccagtgcggt gtaccaaccc
     1021 tcgccgacct acctgaactc actcgccgcc gtcgcagcaa acgagtcgat cgcgccgtac
     1081 ctcgcgctgc ccgcaggcct tccaccgcaa gtccaccaga tcgccgacga gatcgtcgcc
     1141 aacgccggcg ccgtcagtca gtaccagaaa gcgatcgcgc tccaggcttg gttccaggcc
     1201 ggcacgtacg acatctcggt gaagacagga acgagcgaga ccgcgctgct gacatttctc
     1261 agcaaccgtc gcggtttctg cgagcagttc gccgcgacca tggcgttgat ggcccgcctc
     1321 gaaggtattc cggcaagggt cgatgtcggg ttcacaccag gaacgaaggt cgctaactcg
     1381 aactcatacc tcgtgaccac cgcagatgcg cacgcgtggc ccgagctgta cttccccggc
     1441 gccgggtggc tgcgcttcga accgactcct cggtccgacg ggcaaaccag cgacccctcg
     1501 tacgcaaacg ccgcggtcgg cccacagccc gcggcgtccg gcaccgcggt gccgaatgac
     1561 gctgtagcca agccccaaat ccccaacttc catacggggg ggacccccgc ggcaagcaag
     1621 tcgcggcacc acggtttcgc gctcggcaag ctatcggcgg gctggctgac agcggtgctc
     1681 gtcgtcctgc tcggcctggt cgtggcaccg ctcacgcgct ggacaacccg tcgacgacgg
     1741 tggaccgcag ccgacaccga ggcggcccga gcgcacgccg cctgggacga gctcggtgac
     1801 gacgtgcgcg atctgggtct cgaatggggc ggcgacagcg atacgccgag acgcgcggcc
     1861 gcgacgctcg tcgcgacccg ccgactggcc tacgacgtcg ccgcacagca ggcgctctcg
     1921 cgactcacga gggccgagga gcttgcccgt tacgcggcac gcccggacga acttcgatgg
     1981 gtcgatcaag atccgcgagc cgacgagaag gcggttcgca aggctctgtt cacctcagtt
     2041 tcgcgcctcc gccgactgcg ggctcgcgtg gcgccaccct cgacgggtcg cctcgcggcg
     2101 aacgccacgg cgtggatcgg cgacacctgg cacgggttca cggctgggtc tgggcggtgg
     2161 gtcgggcgcc agttcaaccg tcgtgccgat cgcgacggtc aggactaaac ggaccgtcaa
     2221 aaggaatacc cgaccagtca cgactagcag aagctagtca ggacgcccgt cggagtcgtc
     2281 acgcccgccg agactgtcgc ggcgacgatc ccaacgctct tcgaatcgtt ggacgaggcc
     2341 ccgcttgacc accggtcgcc cgaccggctt ctcaccgcga cggcgacggg cttgggtgaa
     2401 ctccgcgggc cgcaccacgc cggcgccggg cgccgcggcg gcgtcaggga cctcgcgcgt
     2461 ctggcgccag gtggacgctc cgtacagaaa cgccaacagc atcaacagga agccgacgac
     2521 gctgagttcg agttgttgga ccgcgatacc gactgggagc aggacgacgc ccacgatgcc
     2581 gccgatggcc gcccgcagat agcggcggcg atgcaacgta cgggggtcga tgccgcgaac
     2641 ggtggcggcg aactttggat cgtcggcgta gagggcacgt tcgatctgct cgagcactcg
     2701 ctgctcgtgc tcagagagcg gcacgctgcc ctcccaacaa catccccacc aaccatctga
     2761 cccgcttgcg ccagatgttg cctaccagtg tgcaacgaca gtgcgacaac agcgaacagg
     2821 cggggccgat gggccgaacg acgaccgggt cggccgccgc tacctcagga tacgagcctg
     2881 gcgggagcgg cgaaagtcgc atccgcaggc tatgacgaga tcggtacacg atcgggacct
     2941 ctggagtccg ccaggtggct tcagtatcgg cccgcacccg tcagatctgg agagctcccg
     3001 atagcccaat ggccgcgact aaccacgccg gccgacgacg tgcagctgcg tggcgatctc
     3061 gcggaacgca gggatttctg acgcctcgaa ctccaacgcg atcaaggtct cgacggcggc
     3121 cgggtcggcg tccagcgatc ccccaggaac cagatcggcg aacactcgcg agccgtgggt
     3181 gccgaccact gtgagaccag ccatcgccag cagtgcctca agatccgcca aagcaaagcg
     3241 gcggggcgcc gggtcggtgt cggaatagcg tccgacggga tcacgcaggg cgtggagcgc
     3301 ttcgtcgaaa cggcccgccg cggcgcggtg caagaccgcc gccaaccgat tcgcggcgac
     3361 gacactggtc acgccgcccg gccgcaggac tgcggtcacc gctgtgagta cggccgtggg
     3421 atcgtcgacg acttcgagca cgctgtgcag gacgaccaga tcgaacgatg cgggccggac
     3481 aacgtcgagg aggctggcca catcgccctg gacactgcga acccgatcgg tcacgcccgc
     3541 gtcggcagca cgtcgatcaa gggcagccag cgaatcagga ctcgcgtcga caaccgtgac
     3601 gtggtgcccc aactcggcca gcggcaccgc gaatccgccg gtcccaccac cagcatcgag
     3661 gacgtcgagg tgcacgcgtc ctgtttcggc gaccatgtcg accagcaaag atcgaacgct
     3721 gcgcaaaacc acagccgtgc ggaccgagcc acgcgttcgc aatcggtcgg ccatgaggcg
     3781 caccctatcc gtcagtcggc gatcggggtg gcccagccgg ggcgctggat gaggcagaac
     3841 ggatgcccgg ccggatcagc gaagacattg tcgccgtcga gttttatcgc gcccagagaa
     3901 agcacccacg gcccggcgtc cgccacgctc tcgaccatga tgtcgaggtg cgcttgctgc
     3961 ggaaccgcac tgtcgggcca ggtcggagcg cgatggtctg gcgcgcgctg aaatgcgatc
     4021 cctgacgtcg tctcgttggc tgcgacgacg acgaagtcgg cacttgcgta ggtgatcggc
     4081 tggttcagca gggtcgagta gaaggtcgcg attgctcccg gatcggggca gtcgaagatg
     4141 acgtggtgca agcgtccgcg catgtcgtcg atcgtttcag gtgcgggact gattctctgc
     4201 tcagcttgcc cggatcgtgt cgaccggcag cgtcggctgg tggataacgc ccatcgtggt
     4261 ttcgaccaag gcaaggaaaa tctcggcatc gcggacgagg tcgtcggact cacgcgcggt
     4321 gactgcgcca tcgagcccgg cttcggcagc ggcccgcttg cctgcgccag cggcaaacag
     4381 ggcggcccat tcggcaagct cgggcgcgac ctgggtgagc agtacccagg cattgcgcga
     4441 tcgacggact cggcgcactc ccggaatctc tggccgggcg cgcgcagcga gcactgccgc
     4501 cgcggcgcgg agagccgcaa ggtgagcact tgcgtagcgc tgcgcgggcc gagaactggt
     4561 cgcggcttca tccaacccgt ggcgggcgga cgcgaggagg ccgagagccg cgggcagatt
     4621 cgcgcgctgg acgcgctgga caggctggac ccgctcaaga gtcgagctca tcgatgtcat
     4681 cgtcatatcg ccggcctcct tacgagcggc tcgatagttg gcgccagcag cagcagccag
     4741 cagcagcagc agctgtccgc tgctggcgcc cgagccacga tgggtcgagg accgtggccc
     4801 gggcgccagt g
//
LOCUS       DASWVF010000758         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_148724, whole genome shotgun sequence.
ACCESSION   DASWVF010000758 DASWVF010000000
VERSION     DASWVF010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148724"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="paaC"
                     /locus_tag="VGD55_13425"
     CDS             complement(<1..440)
                     /gene="paaC"
                     /locus_tag="VGD55_13425"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY0871396.1"
                     /translation="MSEGTVDPMAAPLFTYALRLGDDALVLSQRLSEWASRAPEIEED
                     IALTNIALDLLGQARALLDYAAVVEGGTRDEDDLAYVRTEREFCNVHLVEQENGDFAH
                     TIARQVFFSTYQLFLYEELINSTDELLASVAAKGVKEAAYHHDHA"
     gene            654..2021
                     /locus_tag="VGD55_13430"
     CDS             654..2021
                     /locus_tag="VGD55_13430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0871397.1"
                     /translation="MSRLPLRPPPKARSRATIMHADLDAFYASVEQIDKPSLRGKPVV
                     VSGLGLRGVVATASYEARVHGVRSAMSTMEARGRCPKAAFLAPRFEAYRAASVIVMGL
                     LHDLSPLIEPLSLDEAYVDLEAGAEAVTGDLSAPGVRRVAELVKASVFAATGLTLSVG
                     IGSSKLVAKIASDLQKPDGLMVVEPGSEADVLRPLPAGRLPGVGPATAAKLRSIGVTS
                     IADLADLPEAELVRALGNSQGRGLWFLAQGLDDREVTPERESKSVSVEDTFEHDIADV
                     ARLISVVDDMARRVVERLRGTRLSGRTISVKVRLHDFTTLTRSATLPAPTDDVRVVVR
                     LARQLLSEVDVSGGLRLLGVGVSGLADWTQDDLFGELIAADPHDDPDTDGEPFELAPP
                     ARPDRSWVAGRDVSHPTWGHGWVQGSGAGWVTVRFETRHTPPGRVRSFRIADPDLRPA
                     APLPW"
     gene            2078..>2542
                     /locus_tag="VGD55_13435"
     CDS             2078..>2542
                     /locus_tag="VGD55_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY0871398.1"
                     /translation="MSFATPTFLWFFMPLTLAVYWVLPRGARNGVLAVASLVFYTWGG
                     HAFVFLLLGCVAVNFATGIALDSSTPFLQRHRRTALVIAIVVDVGALAVWKYADFASR
                     QVNSLVHDFGGHGSQVLHFVLPIGISFFTFHHLSYVVDVYRGIRKPLRNPLTF"
BASE COUNT          405 a          848 c          820 g          469 t
ORIGIN      
        1 gcgtgatcgt ggtggtacgc agcctctttg acacccttcg ccgcaacact ggcgagcagt
       61 tcgtcggtgg aattgatgag ctcttcgtaa aggaacagtt ggtacgtaga aaagaacact
      121 tggcgcgcaa tggtgtgcgc gaagtcgccg ttctcctgct cgacgagatg cacgttgcag
      181 aactcgcgtt cggtgcgcac gtacgcgagg tcgtcttcgt cgcgcgtgcc gccctcgaca
      241 accgccgcgt agtcgagcaa cgcgcgagcc tgaccgagca gatcaagggc gatattcgtt
      301 agcgcaatgt cttcttcgat ctcgggagct cgcgatgccc actccgacag ccgctgcgac
      361 aagacgagcg cgtcatcgcc gagtcgcagc gcgtaggtga acagcggagc cgccatggga
      421 tcgaccgtac cctcgctcac cggcgtccca cctccgaagg tcgcacgtag caacgcgggt
      481 tttcctcaac catatccacg gatggcgtag cgaatgtgtc tatttcgccg cgtcgcgatg
      541 aacgaatcgc tgcgaatcag cgtgcacccg gccctcgatc atggaaccgc ccggcgtcgt
      601 gtcggcgcga cggtcgaaga taaaggcgtg aaacacgcag gccggacgcc gcggtgagcc
      661 gtcttccgct acgcccaccg ccaaaggcgc gctcacgcgc cacgatcatg cacgccgatc
      721 tcgacgcgtt ctacgcatcg gtcgagcaga tcgacaagcc ctcactgcgc ggtaagccgg
      781 tcgtcgtcag cgggttgggc ctgcgcggcg tcgtcgccac cgccagctat gaggcgcggg
      841 tccacggcgt ccgatctgcg atgtcgacga tggaagcccg cggccgctgc ccaaaggcag
      901 cgttcctcgc gccacggttc gaggcctacc gcgcggcgag cgtgatcgtc atgggcctgt
      961 tgcacgatct gtcgcccctg atcgagccgc tgtctctcga cgaggcgtac gtcgatctcg
     1021 aagccggcgc cgaggccgtg accggcgact tgtcagcacc cggcgtgcgt cgggtggccg
     1081 agctcgtcaa ggcatcggtg tttgctgcga ccggcctgac gttgtcagtc ggcatcggca
     1141 gctcgaagct ggtcgccaag atcgcatccg accttcaaaa gcccgacggg ttgatggtcg
     1201 tcgagcccgg cagtgaggcc gacgtcctgc ggccgctgcc tgccggccgg ctgcccgggg
     1261 taggtccagc gactgcggcg aagcttcgct ctatcggcgt gacctccatc gccgacctcg
     1321 ccgacctgcc tgaggccgag ctggtcaggg cactaggcaa tagccagggg cgcgggctgt
     1381 ggttcctcgc gcaggggctc gacgaccgcg aggtcacacc cgagcgcgag tcgaagtcgg
     1441 tcagtgtcga ggacaccttc gagcacgaca tcgccgacgt cgcgcgcctg atctcggtgg
     1501 tcgacgacat ggcgcgccgc gttgtcgagc ggcttcgcgg cacgcgcctc tcggggcgca
     1561 ctatttcggt gaaggtacga ctgcacgact tcacgacctt gacccgctca gcgacgctgc
     1621 ccgcaccgac cgacgacgtg cgggtcgtgg tgcgtctcgc ccgccagcta ctgagcgagg
     1681 tcgacgtgag cgggggcttg cggctgctcg gggtgggtgt gagcggtctc gccgactgga
     1741 cccaagacga cctattcggc gagctgatcg cggccgatcc gcacgacgac ccagacaccg
     1801 acggcgaacc tttcgagctc gcgccgcccg ctcgccccga tcggtcgtgg gtcgcgggtc
     1861 gcgacgtgag ccacccgaca tggggtcacg gctgggtcca agggtctgga gcgggctggg
     1921 tcacagtgcg attcgaaacc cgccacaccc cacccggtcg agtgcggtcg ttccgtatag
     1981 cagatcctga ccttcggccc gcagcaccgc tgccctggta gcttcccccg accggccgca
     2041 cgacctttag agccccttta gattcaggag gacccggatg tcgttcgcga cgccgacctt
     2101 cctgtggttc ttcatgccac tgacgctcgc ggtgtactgg gtgctcccgc gcggtgcccg
     2161 caacggcgtt ctcgcagtgg cgagcctggt gttctacacc tggggcgggc acgcgttcgt
     2221 cttcttgctg cttggctgcg tcgccgtcaa cttcgccacc gggatcgcgc tcgattcatc
     2281 gacaccgttc ctgcaaagac atcggcggac ggcgctcgtg atcgccattg tcgtcgacgt
     2341 gggcgcactc gcggtctgga agtacgccga cttcgcttcg cgtcaagtga actcattggt
     2401 gcacgacttc ggtgggcacg gcagtcaggt gctgcatttc gttttgccga tcggcatttc
     2461 cttcttcacg ttccatcacc tgagctacgt cgtcgacgtt taccgcggca tccgtaagcc
     2521 gttgcgaaat ccgctcacgt tc
//
LOCUS       DASWVF010000759         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_250742, whole genome shotgun sequence.
ACCESSION   DASWVF010000759 DASWVF010000000
VERSION     DASWVF010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250742"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..1582
                     /locus_tag="VGD55_13440"
     CDS             506..1582
                     /locus_tag="VGD55_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0871399.1"
                     /translation="MANGPTGASSYRGPAMTDVARLAGLSHQTVSRVINNHANVRPTT
                     RVRVLAAIEELGYRRNSAARTLVTGRSQTLGVVSLNSTLYGPASTLYGIEQAALDSHY
                     FITVATLRSTDRAAVRQAIGRLVDQGVDGIVVIAPLESVAEALADLPAGLPVVAVEGD
                     ADAEIDTVTVDQRAGARAATQHLLDKGHQTVWHVAGPQDWFEARHRSAGWQHTLEEAG
                     AEVPPPLSGDWSPRSGFEAGRVLARMPDVTAVFAANDSMALGVLRALHDYGRDVPGDV
                     SLIGFDDLPEVAYLTPRLTTVRQDFAEVGRRGLALLVEQIESGVKGARRVVVGTTLIV
                     RDSTREVDRPASSRRRGSTSGGGQ"
BASE COUNT          317 a          603 c          637 g          334 t
ORIGIN      
        1 cgggcaggaa ccccggcgct tacgagtcgg gcccggtaca ggatcggatc cggttgtatg
       61 cgtccgaatt cgggcgtggc cacgtccaaa ttcgaaggcc ttcttcgtcg tgagcgggcc
      121 acgggtagcg gtcccagtgt caatcgacgc cgagtgtgta cgttaacatc cgagccgtca
      181 agacacgatc gagaccgcat gcgggatcgt gtccgtgtcg ttacaatccg atcgcgtgat
      241 gcagctcgtc tgttccgggc gtcataccgg tcggtcagat gcgcaaatcc acgggctgtt
      301 gtgaatggtc cgccgcgttg tcacatcgcg tcagacacgg gaatctgcag cgaatctgag
      361 cccaagcgca gtcgagacag gcagcgttga catcgccatg agccgccctt agtgtccccg
      421 ttatgttaac gctgtccctg gcgtgttaac gctccaatga acggacggtt gcaagtgcgg
      481 aagacccgca tgtacgagtc cggccgtggc taacggcccg acgggagcga gtagttaccg
      541 cggcccggca atgactgatg tcgcccggct ggccggcttg tcacatcaga ccgtgtcgcg
      601 tgttatcaac aatcacgcga acgtcaggcc gaccacgcga gttcgcgtcc tggcggcgat
      661 cgaagagctc ggctaccgcc gcaacagcgc cgcccgcacg ctggtgactg gccggtcaca
      721 gacactcggg gttgtcagtc tcaacagcac gttgtacggc ccggcgtcga ccttgtacgg
      781 gatcgagcaa gcagcgttgg actcgcacta cttcatcacc gtcgccacct tgcggtcgac
      841 ggatcgggca gcggtacggc aggcgatcgg ccgactggtc gaccagggcg tcgacggcat
      901 tgtcgtcatc gccccgttgg agtcggtcgc ggaagccctc gccgacctgc ccgcgggcct
      961 gccagtggtg gcagtcgagg gcgacgctga cgcagagatc gacaccgtca cggtcgatca
     1021 acgggccggt gcgcgagcgg ccactcaaca cctgttggac aagggccatc aaaccgtctg
     1081 gcacgtcgcg gggccgcaag actggttcga ggcacggcat cgctcggccg gttggcagca
     1141 cacgttggag gaggccggcg ccgaggtgcc gccgccgctg tcgggtgact ggagcccgcg
     1201 ttccgggttc gaggccggcc gggtgcttgc gcggatgccc gacgtcacgg cggtcttcgc
     1261 cgccaacgac tcgatggcat tgggcgtctt gcgcgcactg cacgactacg gccgcgacgt
     1321 gcccggcgac gtcagcctga tcggtttcga cgaccttccc gaggtcgctt atctgacacc
     1381 gcgattgacc accgtgcgac aagacttcgc ggaggtcggc cgtcgcggct tggcgcttct
     1441 cgtcgagcag atcgagtcgg gcgtcaaggg cgccaggcgc gttgtcgtcg gtacgacgct
     1501 catcgtccgc gacagcaccc gcgaggtcga tcgtcccgcc agctcgcgcc ggcgcgggtc
     1561 tacctcgggg ggcgggcagt agtcatgaga tctggccact gctggcttcg agcctgcgat
     1621 gttagcgcta agaataatcg cgctatcgtg agtgacttag cgctccgacg acaagctgcc
     1681 gcctcacacg ggccgcagca cgctacttcg cgatgggcgg acccagcacg gtgagcaacg
     1741 caacctacgt cgtgggtgtc gatttcggga cactgtctgg tcgcgcgctc gtggtccggg
     1801 tagcggacgg cgcggagctc ggcactgcgg tccatgaata ccgtcacggg gtgctggaag
     1861 gcgcgctgcc atctggtcgg cgactcccac c
//
LOCUS       DASWVF010000760         7334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_480360, whole genome shotgun sequence.
ACCESSION   DASWVF010000760 DASWVF010000000
VERSION     DASWVF010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480360"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VGD55_13445"
     CDS             <1..842
                     /locus_tag="VGD55_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0871400.1"
                     /translation="ERHDRRDIPMTESATSGLPQDAMTRLSELQPGKPGSIFTSDLTV
                     NEFLLVKQAGFTPLGMVLGSSIYHVGIQIGRWSQNQELDVLSQAMYHARELAMSRMEQ
                     EANVLGADGIVGVRLEVEFKEWGADIAEFLAVGTAVKADDGTSWRNDKGLPFTSDLSG
                     QDFWTLVQTGYAPLGMVMGTCVYHIAHRGLGNVLSSVGRNTELTQFTQALYDARELAM
                     ERMQKEAEELHAEGIVGVQLHQRNHSWGAHTTEFFSIGTAVRPLRADHVITPPSMVVT
                     LDN"
     gene            845..2203
                     /locus_tag="VGD55_13450"
     CDS             845..2203
                     /locus_tag="VGD55_13450"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0871401.1"
                     /translation="MPTRLSVDATPVARGLRSAIDLDALAVAAPLLTLLIVAILREGA
                     FFRPDIVVSPALVLALALSTPQVRAWCRQNLAAVAAGVIATGWWIGDALLSHHSVDSW
                     RLPSAWLCMAAGYGCARSLTPAGRRVAQGGVAVTGVALAIAGLVLVGVRATLWTLPDE
                     RSLRFSGPFTYPSAVGLFLVLALIAGTEFSAARPVTFARAVTVLGIVATDSRGAVVGL
                     AVALCFRNVRRELGTAVVAAIIGAPLLLLGQRAHDSHVLVFAAVVVAFGLTLIPDAVV
                     RRFVRFAAIPAAGVVGWLLVTQHHAVSGFDASWTERGDILRGAAHVFRAHPLLGAGPD
                     PAFAAHTLTGQAGIAYFTHNEPVEIAISVGILGALVLTICAVIVARALARGRGELAVP
                     VLASVAAAGLVDFVWHFPALGLAAGIVAGASTVGAWNKQAGNRAQQPAPHPPTPSNPS
                     AS"
     gene            2125..2781
                     /locus_tag="VGD55_13455"
     CDS             2125..2781
                     /locus_tag="VGD55_13455"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0871402.1"
                     /translation="MEQTGGQPGAAASAAPADAEQPVGELVVLRHGATQWSTAGKHTG
                     RTDVPLSLQGEQQAEMVGALIAGRTFALVLASPLQRAQLTAELAGLRRFDTDADLVEW
                     DYGGYEGMTTAEIRRIIPGWSLWRDGVTSRADGQPGETATDAGARADHVIARVLPVLG
                     SGDVALVSHGHFLRVLAARWLSLPPTGGALLALDTASVSVLGFEHGAHVLRHWNLLAR
                     "
     gene            complement(2795..3016)
                     /locus_tag="VGD55_13460"
     CDS             complement(2795..3016)
                     /locus_tag="VGD55_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871403.1"
                     /translation="MSNQGLDPNFPLPPVDEDSDLVGENNERGLPDDDPNGAEAPLPG
                     ITLPNQGAGSYPLPGVRLADELEGIGGEG"
     gene            3115..3789
                     /locus_tag="VGD55_13465"
     CDS             3115..3789
                     /locus_tag="VGD55_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718958.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0871404.1"
                     /translation="MAEDDQGYPGARAYVPAAADLEQLRLAALHCLGCPLHAGATQTV
                     FGSGPATARLVFVGEQPGDQEDRRGLPFVGPAGILLNRALRDAGIDREQTYVTNAVKH
                     FKFTQDGDGKRRIHQTPDQREVVACRPWLAAELRTIDPDVIVALGATAGKSLMGNDFR
                     VTRSRGVALPWPPPGMAAADRRQIVLVATVHPSAVLRADDRDVAYAGLVTDLEIAARA
                     LAGLKR"
     gene            complement(3795..4508)
                     /gene="pdxH"
                     /locus_tag="VGD55_13470"
     CDS             complement(3795..4508)
                     /gene="pdxH"
                     /locus_tag="VGD55_13470"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0871405.1"
                     /translation="MDAEQVAALRAHNASSGLDATDLAADPITQFRRWHEEIVSLGLP
                     EPNAMVLSTASTVETASTVGAMSGDVDAQPSSRHVLLKEVGPEGFVFFTNYGSRKGRE
                     LAANPRASLCFPWFSIGRQVVVTGVVTKVTRAETDAYFATRPRASQLGAWASEHQSEV
                     VASRAVLDQAYVDAAARFAGIDPIPAPPFWGGFRLAPATVEFWQGRDARLHDRLRYLR
                     MDAMDGGASPPWFIQRLSP"
     gene            4657..5760
                     /locus_tag="VGD55_13475"
     CDS             4657..5760
                     /locus_tag="VGD55_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEY0871406.1"
                     /translation="MSDFVPGLEGVIAFETEIAEPDRDGGSLRYRGVDIEQLVGQTSF
                     GDVWGLLVDNSFTPGLPPFNNLTLPVHSGDVRVDVQAGLATLAPKLGFKPILDLDDTQ
                     VRDELAQTSVTALSYVAQSARGDATPVPQSEVEKGTTAVEQFLLRWRGELDPLKVKAL
                     DAYWVSAAEHGLNASTFTARVVASTGADVAASLSAAVGAMSGPLHGGAPTRVLHMVED
                     VEKTGDAEAYVKDLLDKHERLMGFGHRVYRAEDPRARVLRRVAQELGSPRYEAASALE
                     QAALKELRTRRPDRPIETNVEFWAALILDLADVPAPMFAAMFTCARVAGWSAHVLEQK
                     RTGRLVRPSARYIGPGTRPASSVPGWEIAHQHI"
     gene            complement(5866..6756)
                     /locus_tag="VGD55_13480"
     CDS             complement(5866..6756)
                     /locus_tag="VGD55_13480"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY0871407.1"
                     /translation="MPMPKPIPAALQDQPFSTAVAREAGVSDWQLAGARYGSPFHGVH
                     WVGTPEGLATRCRAALVVVRLPAVFSHVTLARLMALPVLSGDERVHVTVAPGRAAPRH
                     RGMVGHVSAYAAEETTELFGLPVTTAGRLFSDLAATFSRPSLLAIGDAILRGRHATVA
                     ELRNVLESRVGRRGSRRAIALLPLLNAAAESPMESVVRLLLFDGGLAVPEVNANIYDT
                     LGRFLARADLLFRAARVIVEYDGDQHRTSREQFARDVARGSELAAAGYLVLRFTAAAV
                     YGRPAYVLATVRAALASRGF"
     gene            7071..>7334
                     /locus_tag="VGD55_13485"
     CDS             7071..>7334
                     /locus_tag="VGD55_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY0871408.1"
                     /translation="MTDATQMLQIPADLKPTDGRFGAGPSKIRPESLAALAKVGNTYL
                     GTSHRRPGVKHVVGQIREGLGQLFELPGGYEVVLGNGGATAFWD"
BASE COUNT         1214 a         2462 c         2444 g         1214 t
ORIGIN      
        1 ctgagcgcca tgaccgaagg gatatcccga tgacggagtc ggctacgagc ggcttgccgc
       61 aggacgcgat gacgaggttg tcggagctgc agccaggcaa gccaggctcg atcttcacgt
      121 ccgacctgac ggtgaacgaa ttcctgctcg tcaagcaagc tggcttcaca ccgcttggca
      181 tggttctcgg ttcgtcgatc taccacgtcg ggatccagat cggccgttgg agtcagaacc
      241 aagaactcga cgtgttgtcg caggcgatgt atcacgctcg cgaactcgcc atgagccgca
      301 tggaacaaga agcgaacgtg ctcggtgccg atggcatcgt cggcgtcaga ctcgaagtgg
      361 agttcaagga gtggggagcc gacatcgcgg aattcctcgc ggtgggcact gcagtgaaag
      421 ccgacgacgg cacgagttgg cgcaacgaca agggcctgcc tttcacctca gacctgtcgg
      481 gccaagactt ctggacgctc gtgcagaccg gctatgcgcc actcggcatg gtgatgggca
      541 cctgcgtcta ccacattgct catcgcggcc ttggcaacgt gctgtcgagc gtgggccgca
      601 acaccgagct cacgcagttc acccaggcgc tgtacgacgc gcgcgaactc gcgatggaac
      661 gcatgcagaa ggaagcagaa gagctgcatg ccgaaggtat cgtcggcgtg cagttgcatc
      721 agcgcaatca ctcctggggt gcgcatacga ccgagttctt ctcgatcggc accgcggtca
      781 ggccgctacg tgccgaccat gtgatcacac cgccgtccat ggtcgtgacg ctcgacaact
      841 gatcgtgccg acgcgattgt ctgttgacgc aacccctgtg gcgcgaggtc tgcgcagcgc
      901 tatcgatctt gatgcgctcg cggtcgccgc tcctctgctc acgctgctga tcgttgcgat
      961 cctgcgcgag ggcgcgttct ttcggcccga catcgtggtg tcgcctgcct tggtgctcgc
     1021 actcgcgctt tcgacaccgc aggttcgagc ctggtgtcgc cagaatctcg cggccgtagc
     1081 agccggcgtc attgcaaccg gatggtggat cggcgacgcg ttactttctc atcacagcgt
     1141 cgattcgtgg cggctgccgt cggcgtggct gtgcatggcc gccggatacg gctgcgcgcg
     1201 gagtctgacg cccgccggac ggcgagtggc acagggcggt gttgcggtca cgggtgtcgc
     1261 gctcgctatc gcaggattgg tactggtcgg cgtccgcgca acgctttgga ctttgcctga
     1321 cgaacggtcg ctgcggttct ccggaccctt cacctacccg agcgcggtcg gccttttcct
     1381 ggtgctcgcg cttattgccg gcactgagtt ttcagccgcg cggcccgtga cgtttgcccg
     1441 cgcggtcacc gtgctcggga tcgtcgcaac cgacagtcgc ggcgctgtcg tcggtctcgc
     1501 ggtcgcactg tgcttccgca acgtcaggcg cgaactcggc accgcggtcg tggcggcgat
     1561 catcggcgca ccgctcctgc tgctcggaca gcgcgcgcat gacagtcatg tcctcgtgtt
     1621 cgcggcggtc gtcgtcgcat tcggtctcac cctcataccg gacgccgtgg tgcggcggtt
     1681 cgtgcgattc gctgccattc cagccgccgg ggtagtcggc tggctcctcg tgacgcagca
     1741 ccacgcggtc tcggggttcg acgcgagctg gacagaacgc ggcgacatcc tgcgcggtgc
     1801 cgcccacgtc ttccgcgcgc acccgttgtt gggagcgggc cccgatccgg cgttcgccgc
     1861 gcatacgctc acgggccagg ccggcatcgc ctacttcacc cacaacgagc ccgtcgagat
     1921 cgcgatctcg gtcggcatcc tcggcgcgct cgtactcacg atctgcgctg tgatcgtcgc
     1981 gcgggcgctc gcacgaggcc ggggggagct cgccgttccg gtactggcga gtgtcgcggc
     2041 tgcggggctc gtcgacttcg tctggcactt tcccgcgctc gggctcgcag ccgggatcgt
     2101 ggccggcgcc agtacggttg gcgcatggaa caaacaggcg ggcaaccggg cgcagcagcc
     2161 agcgccgcac ccgccgacgc cgagcaaccc gtcggcgagt tagtcgttct tcgacacggc
     2221 gcgacgcagt ggagcactgc cggcaaacac accggccgca ccgacgtccc gctcagtttg
     2281 cagggcgaac agcaagcgga gatggtcggc gcgttgatcg cgggtcgcac gtttgcgtta
     2341 gtgctcgcga gcccgctgca gcgcgcgcag ttgaccgcag agcttgcggg cctgcggcgt
     2401 ttcgatactg acgccgacct cgtcgagtgg gactacggcg gctacgaagg aatgacgacg
     2461 gctgagatcc ggcgaatcat cccgggctgg tccttgtggc gcgacggcgt gaccagtcgc
     2521 gccgacggtc agccgggcga aacggcgacc gacgccggcg cgcgggccga ccacgtcatc
     2581 gcgcgcgtgc tcccggtgct cggctcgggt gacgtcgcgt tggtgtcaca cgggcacttc
     2641 ctgcgcgtgc tcgctgcgcg gtggctctcg ctaccgccga ccggcggcgc tctcctggcg
     2701 ctcgacaccg cgagcgtttc ggtgctggga ttcgagcacg gcgcccacgt cctgcggcac
     2761 tggaacctgc tggcgcggta gccgaatcgt gctgctagcc ctcgccgccg attccttcca
     2821 gttcgtcggc aagccgcacg cccggcagcg gatatgaccc ggcgccttgg ttgggcaacg
     2881 tgatgcctgg cagcggtgcc tcggcgccgt tcgggtcgtc gtccgggaga ccgcgttcgt
     2941 tgttctcccc gaccagatcg gagtcttcgt cgaccggcgg cagcgggaaa ttcgggtcaa
     3001 ggccttggtt gctcatgaat cctcctcagc acgcacacag ccgcctcaca cggcggatcg
     3061 gtgcacgtcc taccctgcgt tcgacaacgc ggaacaccgg taaggaagat ttccgtggca
     3121 gaagacgatc agggctaccc gggcgcgcga gcgtacgtgc ccgccgcagc cgatcttgag
     3181 caactgcggc tggccgcgct gcattgcctg ggttgcccgt tgcacgcggg tgcgacgcag
     3241 acggtcttcg gatcggggcc agcgacggcc cggctcgtgt tcgtcggcga gcagccaggt
     3301 gatcaggagg accggcgcgg tctgccgttc gtgggcccag cggggatcct gctgaaccgg
     3361 gcgcttcgcg atgccggcat cgatcgcgag cagacctacg tcacgaacgc ggtgaagcac
     3421 ttcaagttca cgcaggacgg cgacggtaag cgccggattc atcagacgcc ggatcagcgt
     3481 gaggtcgtgg cgtgtcgacc atggctggcc gccgaactgc ggacaatcga tcccgatgtg
     3541 atcgtcgcgc tcggtgcgac agccggaaaa tccttgatgg gcaacgattt tcgggtcacc
     3601 cgttcacgcg gagtggccct gccctggccg ccgcccggga tggccgctgc cgaccgtcgg
     3661 cagattgtgt tggtagcaac ggtccacccg tccgccgtgc tgcgggccga cgaccgcgac
     3721 gttgcgtacg ccggtttggt caccgatctg gaaatagcgg cacgggcact cgcggggttg
     3781 aaacgctgac gcagctaggg cgacaaccgt tggatgaacc agggaggtga cgccccgccg
     3841 tccatagcgt ccatccgcag gtagcgcagg cgatcatgca ggcgagcatc ccggccctgc
     3901 cagaactcca cagtcgccgg cgccagccgg aacccgcccc aaaacggtgg cgccgggatc
     3961 gggtcgattc ctgcgaacct ggccgctgcg tcgacgtacg cctggtcgag cacggcccgg
     4021 ctggccacga cctccgactg atgctcgctc gcccacgcgc cgagctgcga cgcacgcggt
     4081 cgggttgcga agtaggcgtc ggtctcggcc ctggtgacct tggtcacaac accggtcacg
     4141 acgacttggc gtccgataga aaaccaggga aaacacaggc tggcacgcgg attcgccgcc
     4201 agttcacgac ctttgcgaga gccatagttg gtgaagaaca cgaagccttc cggcccgacc
     4261 tccttgagca gcacgtgacg actgctcggc tgcgcatcga cgtccccgct catagcccct
     4321 acggtcgacg cagtctctac ggtcgaggca gtcgagagga ccatcgcgtt cggctcgggc
     4381 agtccgaggg aaactatttc ctcgtgccag cgacggaact gcgtgatcgg gtcggccgcc
     4441 aggtcggtgg cgtcaagccc ggagctcgcg ttgtgggcgc gcagggcggc cacctgttcg
     4501 gcgtccatcg gagcacctcg gcattctcag caggacctgc tcgttcaacc cgcacagtag
     4561 cggggacaat gtgacgaccg gtgaagaccg gtgaagaccg gtgaagaccg ccgcgcgtga
     4621 cccgttggcc cgtgaacaga gcgaggagca gcgctgatgt ccgacttcgt cccaggcctt
     4681 gaaggggtca tcgcgttcga gaccgagatc gccgaacccg acagggacgg tggctcgctg
     4741 cgctaccgcg gcgtcgacat cgagcagttg gtcggccaga cgtcgttcgg tgacgtgtgg
     4801 gggctgctgg tcgacaactc cttcaccccg ggcttgccgc cgttcaacaa cctcacgctg
     4861 cccgtgcact cgggcgacgt ccgggtcgac gtccaggcgg gccttgccac cctggctccg
     4921 aagctcggct tcaagccgat cctcgacctc gacgacacgc aggttcgcga tgagttggcg
     4981 cagacctctg tgacggcgct gtcgtatgtc gcgcagtcgg cgcgcggcga cgcaacgcca
     5041 gtaccgcaaa gcgaagtcga gaaggggacg acggcggtcg agcaattcct ccttcgctgg
     5101 cgcggtgaac tcgacccgct gaaagtcaag gcgctcgacg cctactgggt ttctgcggcc
     5161 gaacacggcc tgaacgcctc gacattcaca gcacgtgtcg tcgcctcgac cggcgctgac
     5221 gtcgcggcgt cgctgtcagc agcggtcggc gccatgtcgg gaccgttgca tggcggcgcg
     5281 ccgacgcggg tgctgcacat ggtcgaggac gtcgagaaga ccggcgacgc agaggcgtac
     5341 gtgaaagacc tgctcgacaa gcacgagcgg ctgatgggct ttggccaccg cgtctaccgc
     5401 gccgaggatc cccgcgcacg ggtgttgcgc cgggtcgccc aagaactcgg ctcaccgcga
     5461 tacgaggccg cgtcggcact cgagcaagcg gcgctcaaag agctgcgcac ccgccgtccc
     5521 gaccgcccga tcgaaaccaa cgtcgagttc tgggccgcgc tgatcctcga tctagcggac
     5581 gtccccgcgc ccatgttcgc ggcgatgttc acctgcgcgc gggtggccgg ctggagcgcc
     5641 cacgtactcg agcagaagcg caccggacgc ctggtgcgcc cgtcggcccg ctacatcggc
     5701 cccggcaccc ggccagcgtc gtcggtcccc ggctgggaga tcgcgcacca gcacatctag
     5761 cgccggcgtc ccgcacctgc gctggtcccc aggttcccgg tgatcatgca caaagtaggt
     5821 agcggacgcc gtcgacgtac cacatttgtg cgtgttgatc atgggtcaga aacctctcga
     5881 cgccagcgca gcccggacgg tcgccaatac gtacgcgggg cggccgtaca cagcggccgc
     5941 cgtgaatcgc aggaccagat atccggccgc cgccagttcg ctcccacgtg cgacatcgcg
     6001 cgcgaactgc tcgcgactgg tccggtgctg atcgccgtcg tactcgacga tgaccctggc
     6061 cgctcggaat agtagatcgg cgcgtgcgag gaagcgcccg agagtgtcgt aaatgttggc
     6121 gttgacctca ggaaccgcga gccccccgtc gaacaacagc aatcgaacga ccgactccat
     6181 aggtgactcc gccgccgcgt tcagcagtgg caacaacgcg atcgcgcgcc ggctgcctcg
     6241 gcgtccaacc cgagactcaa gtacgtttcg aagctcggcc accgtcgcat gtcggccacg
     6301 caaaatcgcg tcgccgatcg cgagcagcga cggccggcta aaggtggccg caaggtcgct
     6361 gaatagtcgt cccgcggtgg ttactggcag gccgaacaat tcggtcgttt cctcggccgc
     6421 atacgcgctg acgtgtccga ccatcccgcg gtggcgagga gccgcgcgtc ccggagcgac
     6481 agtcacgtga acacgctcgt caccactcag caccggcaac gccatgagac gggcgagtgt
     6541 cacgtgactg aaaaccgctg gaagacgcac aacgaccagc gccgcgcggc accgggtcgc
     6601 gagcccttcg ggagtgccga cccagtggac accgtggaac ggcgagccat accgcgcacc
     6661 ggccaattgc caatcggaga ccccagcttc acgggccacg gcggtggaga agggctggtc
     6721 ctggagcgcg gcgggaatcg gtttcggcat cggcatcagg ccaacctgga cgggtttggc
     6781 cacgtggggt cggtactcga cgtcggctgt ggacaaggtt gccgtcgtgc acaggcgccg
     6841 taccttcaac gactgcgcac aaagtaggca gcgaagccct ctcgagtgcc tacttcttgc
     6901 atgatcacca acaaacgaag gggcggacgg cggggggctg gggtccgacg gccgcagccg
     6961 ggctccgagc gcccgaaccg agtcgcaatc gccaccgcac acctgcgaca atgggaacgc
     7021 atacggcagc gtcgccgaac ccacccgctc accgatccat ggagctttcc gtgaccgacg
     7081 cgacgcagat gttgcagatc ccggccgacc tcaagccaac cgatggccgg ttcggcgccg
     7141 gcccctccaa gatccgtcct gagtcgctcg ccgcgctcgc aaaggtcggc aacacctacc
     7201 tcggcacctc gcaccggcgg cccggcgtga agcacgtcgt cggccagatc cgcgaaggcc
     7261 tcgggcaact gttcgagctt cccggcggct acgaggtggt tctcggcaac ggcggcgcga
     7321 ccgccttctg ggac
//
LOCUS       DASWVF010000761         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_97926, whole genome shotgun sequence.
ACCESSION   DASWVF010000761 DASWVF010000000
VERSION     DASWVF010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97926"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VGD55_13490"
     CDS             complement(<1..1390)
                     /locus_tag="VGD55_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0871409.1"
                     /translation="MNPTGVLTAQSAPTLHQRRRLGDVLLERGLLTPEQLQDALASQQ
                     SLVGAQRKRLGKLLVDLGFLTERQVAESLAELLSLDILESSDLLVPLEVARLLPRQVA
                     ERAQVLVLGRTSTGLRIATSDPTNVVALDDVRAYTGSHALTLVVATASMIQKQIARVW
                     SLREDPSGMSDSDDIETDTLSEEEEIIHVADQAPTVKLVNQILAEAVRVGASDVHVEQ
                     QPDGIWIRHRIDGVLRDVTRIPKGGAAALVSRLKIVSGMDIAQRRLPQDGRMKITANG
                     VAVDARVSTLPSINGEKVVIRLLPEAEEITPLEKLGLEPDQLEALVSASTASQGLVLI
                     TGPTGSGKTNTLYSLLAEVATRDKNVITLEDPVEIQLPGITQVQVNERAGMTFARGLR
                     SMLRQDPDVILVGEVRDTETALLALEASLTGHLVMSTLHTNSACAAVTRLVDMGVEPY
                     LVASSPALVVGQR"
     gene            complement(1472..2383)
                     /locus_tag="VGD55_13495"
     CDS             complement(1472..2383)
                     /locus_tag="VGD55_13495"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0871410.1"
                     /translation="MRLLPRRVMARASVHAALLATTSVVVCVAALLPATPATAVTYAA
                     VPGLPPTTATAFVVADATTGRILAARDMHGRLAPASTLKVLTALTAFPYLNGSATVMG
                     SPTAAATEGTAVGINAGQAYTAKQLFTAMLVMSANDAAVSIGDAIGGGSKAIARMNAE
                     AAHLQARDTVAHTTNGLDAPGQSSSAYDLALMFRAGLASSRFRYYISQRSAVFPLAKG
                     KSYTIYTHDRLLNSYPGMIGGKNGYTIAAHASFVGAAQRGGHTIIVAVMRDVPDFWPE
                     VASLLDWGFAADGHVKPVGYLAAPRRS"
     gene            complement(2411..3250)
                     /locus_tag="VGD55_13500"
     CDS             complement(2411..3250)
                     /locus_tag="VGD55_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0871411.1"
                     /translation="MTAPYMQHAFLAGTAIAIAAGLIGYFLVLRSQVFTTDALGHVTY
                     TGAMSGLALAFNPLLGLFVATIAVAGGFAVLGRRARADDVVIGSTFAWVLGLGALFQR
                     LYTTRHSSGGNGRAGATYLFGSIFGISSSQAVVSALVSLGVCAVMIFIARPLLFASLD
                     EAVAGARGVPVRVLSAAFLVVVGVTCAQAAQAVGALLLLGLIAAPAGAAHRLTTRPFV
                     GLALAALIALASTWVGLLISYLAPRTPPSFAIIGVASAAYLLAYALTVVTTRRKSLNL
                     PEN"
     gene            complement(3247..4050)
                     /locus_tag="VGD55_13505"
     CDS             complement(3247..4050)
                     /locus_tag="VGD55_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0871412.1"
                     /translation="MFSYPFMVNAFRAGTIVAIVAAVVGWYMVLRKQTFAGHTLSVAA
                     FPGAAAATLIGVSATIGYFGFCLAAAVVIAIASRPERARGFSAESAVVGTVQAFVLAS
                     GYLFATLYNGVLNGLNSQLFGFTFGITNGQVVTLGVVAVVVLGALAIIGRRLFFASID
                     GDVADARGVGVRFLGVTFLLVLGAAAAEASQITGALLVFALLVMPAAIAQRVTARPLA
                     GISLAVVIGVVASWAGLTMAYYTSYPSGFCITTFAFASFIAVRRWGTIT"
     gene            complement(4116..4946)
                     /locus_tag="VGD55_13510"
     CDS             complement(4116..4946)
                     /locus_tag="VGD55_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871413.1"
                     /translation="MTTEPAVQLRDAAVRVGDRLLWHDVNVDVPAGQFVAILGPNGVG
                     KSTLIKAALGLVPLAEGEAFVLGQPAGTANPDVGYLPQRRSFEQSLRVRGIDIVRLGL
                     DGDRFGLPWPWSAKNREAGRRVREVIERVGASGFAGRPIGEVSGGEQQRLLIAQALVR
                     EPRLLILDEPLDSLDLPNQSAIAELIRRVSVDNGVTVLMVAHDVNPILPYLDQVIYLA
                     GGGAVCGSPAEVITSQTLSALYQTPIEVLRTSDGRLVVVGGPEAPAHHADRHSGEPHR
                     "
     gene            complement(4943..5866)
                     /locus_tag="VGD55_13515"
     CDS             complement(4943..5866)
                     /locus_tag="VGD55_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0871414.1"
                     /translation="MRRFGAWPLCAATVIALIAGCATSRHSTNTQGVPLHVVAAENFW
                     GSIASQLGGVKVDAQSIITNPDTDPHDYDPSADDARSIGDAAVVIVNGIGYDPWVSKL
                     LAAAPSSKRRVVNVGDVVGVADGGNPHRWYNPSDVHKVIAAIVAAYKAADPADAAYFS
                     TQQRDYETTGLAQYDDLIAQINKTYAGTPVGASESIFAMVAPTLGLKLLTPPTFLRAI
                     SEGTDPTSADKQTIDTQIKTHAIKVYVYNSQNATPDVQAQVDEAREAGIPVTTITETL
                     SPQTATFQDWQVHELTALQTALAQAAQASGS"
     gene            5978..>6351
                     /locus_tag="VGD55_13520"
     CDS             5978..>6351
                     /locus_tag="VGD55_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0871415.1"
                     /translation="MTTSSGRSTRQKRALAVVIDDAQEFRSAQELHEDLRGRGEQVGL
                     TTVYNQLRALADAGLVDTMRSPDGETLYRRCGSAGHHHHLLCRVCGRTVEVAGTAVER
                     WAEKVAAEAGFVDVSHTMEVVGT"
BASE COUNT         1133 a         2016 c         2142 g         1060 t
ORIGIN      
        1 gccgctggcc gacgacgagg gcgggcgacg acgccaccag atatggctcg actcccatgt
       61 cgacgagccg ggtgaccgca gcgcaggcgc tgttggtgtg cagcgtcgac atgacgaggt
      121 ggccggtcaa ggacgcttcg agggcgagca atgctgtctc ggtgtcgcgg acctcaccga
      181 ccaagatgac atcggggtct tggcgcagca tcgaacgcag cccgcgggcg aatgtcatgc
      241 cggcgcgctc gttgacctgc acctgcgtga tgccgggcag ttggatctcg accgggtctt
      301 cgagggtgat gacgttcttg tcgcgggtcg cgacctcggc gagcaacgaa tagagcgtgt
      361 tggtcttgcc cgatcccgtc gggccggtga tgagcaccaa gccttgcgat gccgtcgaag
      421 cactgacaag cgcttcgagc tgatcgggtt cgagcccgag cttttcaaga ggggtgattt
      481 cctcggcctc cggcaacaac cggatgacga ctttctcgcc gttgatcgac ggcagcgtgc
      541 tcacccgcgc atcgaccgca acgccgttgg ccgtaatctt catgcgcccg tcttggggca
      601 gccggcgctg cgcaatgtcc atgcctgaca cgattttcag ccgactgacg agtgcggccg
      661 cgccgccctt cggtatccgc gtgacgtcgc gaagtacccc gtcgatgcgg tgtctgatcc
      721 agatcccgtc gggttgctgc tctacgtgga cgtcgctcgc gccgactcgc acggcttccg
      781 cgaggatttg attgacgagc ttcaccgtcg gcgcctgatc ggcgacgtgg atgatctctt
      841 cctcttcgct gagagtgtct gtctcgatgt cgtcgctgtc ggacatcccg gacgggtcct
      901 cgcgcagcga ccacactcgc gcgatctgct tttgaatcat cgaggcggtc gcgacgacca
      961 gcgtcagtgc gtgcgatccg gtgtaggcgc gcacgtcgtc gagtgcgacg acgttggtcg
     1021 gatccgaggt cgcgatccgc agaccggtgg acgtgcggcc gagcaccagc acctgcgctc
     1081 gctcggcgac ctgtcgcggt agcaacctcg cgacctccag cggaacaagc aagtccgagc
     1141 tttcaaggat gtcgagcgac agcaactcag ccagtgattc ggcgacctga cgctcggtca
     1201 ggaagcccag gtcgacgagg agcttcccga gccgcttgcg ctgggcgccc accaggctct
     1261 gctggctcgc gagggcgtcc tgcagctgct caggcgtgag caggccgcgc tcgagcagca
     1321 cgtcgccgag ccgccgtcgc tggtggagcg tcggagccga ctgcgcagtg agcacgccgg
     1381 ttgggttcac atagcccttt cggccgaacg cagggccatg tgagcgttcc ggaatccggt
     1441 cttgagcctg ggtcttaggg cctgaacccg ctcaagaccg gcggggcgcg gccaggtagc
     1501 cgaccggctt cacgtggcca tcggcggcga agccccagtc gagcaacgag gccacttcgg
     1561 gccagaagtc gggcacgtcg cgcatcaccg cgacgatgat cgtgtgcccg ccccgctgcg
     1621 cagcgccgac aaagctcgcg tgcgcggcaa tggtgtagcc gttcttgcca ccgatcatgc
     1681 ccggatagct gttcagcagc cggtcgtgtg tgtagatcgt gtacgacttc cctttcgcga
     1741 gcggaaaaac ggcgctgcgc tgcgagatgt agtaacggaa cctcgagctt gcgaggccag
     1801 cccgaaacat cagggcaagg tcataggcgg acgacgattg gccgggcgcg tcgagcccgt
     1861 tcgtcgtatg ggcaacggtg tcgcgggcct ggaggtgcgc ggcctcggcg ttcatgcggg
     1921 cgattgcctt cgacccaccg ccgatcgcat cgcctataga gaccgccgca tcatttgccg
     1981 acatgacgag catggcagtg aagagttgct tcgccgtgta ggcctggccc gcattgatcc
     2041 cgactgccgt gccctcggtc gccgcggccg tggggctgcc catgaccgtg gcggacccgt
     2101 tcagatatgg gaacgcggtg agagccgtca acaccttcag cgtgctcgca ggcgcgaggc
     2161 gcccgtgcat atcgcgagca gccaggatcc ggccggtggt cgcgtcggcc acgacgaagg
     2221 cggttgcggt ggtcggcggc aagcctggga ctgctgcgta ggtaacggcg gtcgctgggg
     2281 tcgcaggcag caacgcagca acacagacga cgacggaggt cgtagcgagc agtgccgcgt
     2341 gcacagatgc gcgtgccatg acgcgtcggg gaagtaaacg catgacccaa ggacggtaac
     2401 cgccgcaggg ttaattctcc ggcaggttga gcgactttcg gcgcgtcgtt acgacggtga
     2461 gcgcgtaagc gagcagatac gcggctgacg ccacgccgat gatcgcaaag ctcggcggtg
     2521 tgcggggcgc gagatagctg atcaacaagc cgacccacgt cgaggcaagg gcgatcaggg
     2581 ccgcgagcgc cagccccacg aacgggcgcg ttgtcagccg gtgagcggct cccgcgggcg
     2641 ccgcgatgag gccgagcagg agtagcgcgc cgaccgcttg cgccgcctgc gcacacgtga
     2701 ctccgacgac caccaagaac gctgcgctga gcacgcgcac gggcacaccc cgcgccccgg
     2761 cgacggcttc gtcgaggctc gcgaacagca acggtcgcgc gatgaagatc atgactgcgc
     2821 agacaccgag cgagaccagt gctgagacaa ccgcctgcga agacgagatg ccgaagatgg
     2881 aaccgaacag gtaggtcgcg cccgcgcgcc cgttcccacc gctgctgtgg cgcgtcgtat
     2941 acaggcgctg aaagagcgcg ccgagcccaa gcacccatgc gaaggtcgag ccgatgacga
     3001 catcgtcggc ccgcgcccgg cgtccgagaa cggcgaagcc gccggcgacc gcgatggttg
     3061 cgacgaagag cccgagcagc gggttgaacg ctaacgcgag gccgctcatc gcgccggtgt
     3121 acgtcacgtg tccgagagcg tcggtggtga agacctgtga gcgcagcacc aggaagtagc
     3181 cgatcaatcc ggcggcgata gcgattgcgg tgcccgccaa gaacgcgtgt tgcatgtagg
     3241 gcgcagtcac gtgatcgtcc cccatcgacg aaccgcgatg aacgacgcga aggcgaaggt
     3301 cgtgatgcaa aacccgctcg ggtaggacgt gtaatacgcc atggtcagac cggcccaact
     3361 cgcgacgacg ccgatgacga cagcgagtga gatgccggcc agcgggcggg ccgtgacgcg
     3421 ttgcgcgatc gccgccggca tgacgagcag cgcgaacacg agcagtgcac cggtgatctg
     3481 gctcgcctca gcagcagcgg cgccgaggac gagcaagaac gtgacaccca agaagcggac
     3541 gccgacaccg cgggcatcag caacatcgcc atcgatagac gcgaagaaca gcctgcgacc
     3601 gatgatggcc aacgcgccga gaacgacgac ggcgacgacg cccagcgtca cgacctggcc
     3661 gttggtgata ccgaacgtga agccgaacaa ctgactgttc aagccattga gcacaccgtt
     3721 gtagagcgtc gcgaacaggt agcccgatgc aagaacgaac gcctgcacag tgccgacgac
     3781 ggccgactcg gcgctgaacc ctcgagctcg ttctggcctc gacgcgatgg caatgacgac
     3841 agcggctgcg aggcagaaac cgaagtaacc gatcgtcgcg ctgacaccga tcagcgttgc
     3901 cgcagcagca ccggggaatg cggcaaccga gagcgtgtgc cccgcaaagg tctgcttgcg
     3961 cagcaccatg taccaaccga cgacagcagc gacgatcgcc acgatggtgc ccgcgcgaaa
     4021 cgcgttcacc atgaacgggt aactgaacat ctctcgaacg tcgtcgagta cattccacga
     4081 ccacgtcgga ctcgcactgc caatggcaag cggcatcatc tgtgaggttc tccgctgtgc
     4141 cgatccgcat gatgcgctgg cgcttcagga ccgcccacga caaccaggcg cccgtcactc
     4201 gtgcgcaaca cctcgatcgg cgtctgatac agcgcggaga gcgtctgcga ggtgatcacc
     4261 tcggcgggtg aaccgcacac cgcgccgccg ccggcgagat agatgacctg gtcgagatag
     4321 ggcaggatcg gattcacgtc gtgcgcgacc atcaacacgg tgacgccgtt gtcgaccgac
     4381 acccgtcgta tcagctcggc gatcgcgctc tggttcggca agtcgaggct gtcgagcggt
     4441 tcgtcaagga tcagcagtcg cggctcgcgc accagtgcct gcgcgatcag cagtctttgt
     4501 tgctcaccac cagacacctc gccaatcggg cgcccggcga accccgatgc gccgacgcgc
     4561 tcgatgacct cgcgcacccg gcgtccggct tcgcgattct ttgcgctcca cggccaaggc
     4621 aggccgaacc ggtcaccgtc gaggccgagc cggacgatgt cgatgccgcg cacgcgcagc
     4681 gactgctcga agctgcgccg ttgcggcagg tagccgacgt cggggttcgc ggtgcctgcg
     4741 ggttggccga ggacgaaggc ttcgccttcg gcaagcggaa cgagacccag cgcggctttg
     4801 atgagcgtcg acttgccgac cccgttcggc ccgaggatgg caacgaattg accggcgggg
     4861 acgtcgacgt tgacgtcatg ccacagcagg cgatcgccga ccctgacggc ggcatcgcgt
     4921 agttgcaccg ctggttctgt cgtcacgagc cgctcgcttg tgcggcttgc gccaacgcgg
     4981 tctgcaaagc cgtgagctcg tgtacttgcc agtcctggaa cgtcgccgtc tgcggcgaca
     5041 gcgtttcggt gatcgtcgtc acggggatgc ccgcctctcg agcctcgtcg acctgggctt
     5101 gcacgtcggg cgtcgcgttc tgtgagttgt agacgtagac cttgatggcg tgggtcttga
     5161 tctgggtgtc gatcgtctgc ttgtcagccg atgtcggatc ggtgccttcg ctgatagcgc
     5221 gtaggaacgt cggtggggtg aggagtttca ggccgagtgt gggtgcgacc attgcgaaga
     5281 tgctctccga ggctccgacc ggcgttccgg cgtaggtctt gttgatctgg gcgatgaggt
     5341 cgtcgtactg cgcgagcccg gtggtttcgt aatctcgttg ctgggtcgag aaatacgcgg
     5401 cgtctgccgg gtcggctgcc ttgtatgcgg caacgatcgc ggcgatgacc ttgtgaacgt
     5461 cgcttgggtt gtaccagcgg tgcgggtttc cgccgtcagc tactcccacg acgtcgccga
     5521 cgttcactac gcgtcgcttg ctcgaaggcg ccgcggcgag gagcttgctc acccacgggt
     5581 catagccgat gccgttgaca atcaccacgg ctgcgtcgcc gatcgagcgc gcatcatctg
     5641 cactcgggtc gtagtcatgc gggtcggtgt cggggttggt gatgatgctc tgggcgtcta
     5701 ctttcacgcc acctagctgg ctcgcaatcg aaccccaaaa gttctcagcc gccacgacgt
     5761 gcagcggcac gccctgggtg ttggtgctgt gccgcgatgt tgcgcagcct gcgatcaggg
     5821 cgatcaccgt cgctgcgcac aggggccatg cgccgaaccg tcgcatctcc cagcccctcg
     5881 gtcaggttcc tcgcttatcc ggaaccgttg caagtacact atcgggaatg gttaccgttt
     5941 ccaagagtga tatcgaccgg ctagatttgg gctcgccatg acaacgagca gcggccggtc
     6001 gacccgccaa aagcgtgcgc tcgcggtggt catcgacgac gcgcaggagt tccgcagcgc
     6061 acaggagttg cacgaagatt tgcgcggccg gggcgagcag gttggtctta cgacggtcta
     6121 taaccagcta cgcgcgctcg ccgacgccgg gcttgtcgac acgatgcgtt ccccggacgg
     6181 ggagacgctg taccgcagat gcggcagtgc cggccaccat caccatctcc tgtgccgcgt
     6241 gtgcggccgg acggtcgagg ttgccggcac tgctgtcgag cgttgggctg agaaggtcgc
     6301 cgccgaagct ggcttcgtcg acgtgtcgca caccatggag gtcgtgggca c
//
LOCUS       DASWVF010000762         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_378135, whole genome shotgun sequence.
ACCESSION   DASWVF010000762 DASWVF010000000
VERSION     DASWVF010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378135"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1665
                     /locus_tag="VGD55_13525"
                     /pseudo
     CDS             13..1665
                     /locus_tag="VGD55_13525"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            1687..>1884
                     /gene="ilvN"
                     /locus_tag="VGD55_13530"
     CDS             1687..>1884
                     /gene="ilvN"
                     /locus_tag="VGD55_13530"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0871416.1"
                     /translation="MSRHTLSVLVENKPGVLARIASLFARRGFNIDSLAVGPTEYPDV
                     SRMTIVVNVEDNPLEQVTKQLN"
BASE COUNT          330 a          636 c          610 g          308 t
ORIGIN      
        1 atcgccttcg ggatcccggg cgttgcgatc ttgccgctct acgatccgct cttcgactcc
       61 gtgaagctgc gccacatcct ggtccgccac gagcagggtg cgggccacgc ggccgagggc
      121 tacgcgatgg cgaccggcaa ggtcggcgtg tgcatggcga cgtccggtcc tggtgccacc
      181 aacctcgtca cgccgatctc gaacgcctat atggactcca cgccgatcgt cgcgatcacc
      241 ggccaggtgc cgtcgtccgc catcggttct gacgcttttc aagaggccga catccgcggc
      301 atcacgatgc cgatcaccaa gcacaacttc ctcgtcacga atcccgacga gattgcgcgc
      361 acggttgccg aggcgttcta catcgcctcg acgggccggc ctggtccggt gctcgtcgac
      421 gtcgcgaagg acgcgctcca ggcgatgacg accttcgact accccgacaa gatcgacctg
      481 cctggttacc gcccggccgc gaagccgcac agcaagcaga tccgcgaggc cgcgaagctc
      541 atcgtgtcgg cgaagcgtcc ggtgctctac gtcggcggcg gcgtgcagat cgcgcgcgct
      601 gatcgggagt tccggcagct tgcggagctg accggcatcc ccgtcgtcac caccttgatg
      661 gcgcgcggcg tgttccccga caaccacccc cagcacatgg gcatgccggg catgcacggc
      721 acggtcggcg cggtcggcgc gcttcagaag tccgacctgc tggtggcact cggcgcccgg
      781 ttcgacgacc gcgtcaccgg caagctctcg acgttcgcgc ccgacgccac gatcatccac
      841 gccgacatcg atccggcaga gatttcgaag aatcggacgg cggatgtgcc catcgtcggc
      901 gacgcacgcg aggtgatcgc tgatctcatc gttgcagtga aggccgaact cgacgcaggc
      961 aaccaggccg atctcacagc ttggtggcgc cagctcgagt cgtggcgcac ggtctacccg
     1021 ctcgggtacg acacgccgtc cgatggcagc ttgtcgccgc agtatgcgat cgagcggctc
     1081 ggcaagatcg tcggttccga cgcgatctat gtggcaggcg tggggcagca ccagatgtgg
     1141 gccgcgcact acctgccgaa tgagaagccc catacgtgga tcaactccgg tggggcgggc
     1201 acgatgggct tcgccgttcc ggctgcgatg ggcgcgaagt tcggcaagcc cgactcgacc
     1261 gtgtgggcga tcgacggcga cggctgcttc cagatgacca accaggagct cgtgacctgc
     1321 gcgctcgaag gtgccccgat caaggtcgcg ctcatcaaca acggcgcgct cggcatggtg
     1381 cgccagtggc agacgctgtt ctacaacgcg cgctacagca acaccgacct gcactcgcgc
     1441 cggattccgg ccttcgtgaa gctggcggac gcgatgggct gcatcggcct gcggtgcgac
     1501 agtgccgacg acgtcgacgc gacgatcgag aaggcgatgg gcatcaacga cgcgcccgtc
     1561 gtcatcgact tcggcgttca caaggatgcg atggtctggc cgatggtcgc ggccggtacg
     1621 agtaacgacg acatcaaggt ggctcgcgac ttggcgccgg cctgggataa agaggagctc
     1681 taagtcatgt cgaggcacac actctcggtg ctcgtcgaga acaagccggg cgtcctggcc
     1741 cgcatcgcga gtcttttcgc gcggcgcggg ttcaacatcg actccctcgc cgtcggcccg
     1801 accgagtacc ccgacgtttc gcggatgacg atcgtggtca acgtcgagga caacccgctc
     1861 gagcaggtca ccaagcagct gaac
//
LOCUS       DASWVF010000763         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_429214, whole genome shotgun sequence.
ACCESSION   DASWVF010000763 DASWVF010000000
VERSION     DASWVF010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429214"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1165)
                     /locus_tag="VGD55_13535"
     CDS             complement(41..1165)
                     /locus_tag="VGD55_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0871417.1"
                     /translation="MKETAITTRDRQQTATRPATDDLEVIRDDAELAVDAGISDISVE
                     ITPDPIDSADTVDAVDAEEPDLDSDEPGVSADPVRNYLNEIGRTALLDAAQEVDLSKR
                     IEAGVYAEHKLSETPKLSPKLRRELEAIRDDGALAKAHMLEANLRLVVSVAKRYSNRG
                     MSFLDVVQEGNLGLIRAVEKFDYTKGYKFSTYAMWWIRQAITRALADQARTIRLPVHV
                     VEQLNKLGRLQRDMHQRFGREPTAEELAEELDRTPEQIEEMLRNARQPVSLDNPVGDD
                     EDTRLGDLIEDSEAPGASDALEYQAMIETVHRALAALEPREARVLALRFGLAEGRPHT
                     LDEIGRDVGLTRERIRQIEKEALAKLRQPDRPDSLLDWAS"
     gene            complement(1285..2544)
                     /locus_tag="VGD55_13540"
     CDS             complement(1285..2544)
                     /locus_tag="VGD55_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0871418.1"
                     /translation="MTATPLLVEPWANRDDLRITAVRAIVTAPEGIALVVVKVETSDD
                     GLYGLGCATFTQRFPAVVAAVEQCVAPLVIGRHPADIEDLTRAVHHSSYWRNGPVLNN
                     ALSGLDMALWDIAGKRLGVPVYELLGGRVRAAAQTYLHAAGHTVEETIEHAATIIARG
                     WTHVRLQTGQPGLGTYGAPGSANAYPGAPYPDGWDVAHYLRTTPELFARARRDLGADV
                     ELLHDVHSRLTPKQAIVLARRLEEHRLFFLEDVVAPEHYDRLAEVRAASPVPIAVGEL
                     LSSVTDAARLVQQHAVDYLRVHVSAVGGLTPARKLAAMCELVGVQTAWHAPGDVSPIG
                     AAVNVALDVTSPAFGIQETHNFAEAVHEVFPGTLTAIGGYLTPSPAPGFGIDLDEDAA
                     RRFPLGAAAAAETSFVLRRPDGAVFAP"
     gene            complement(2541..3443)
                     /locus_tag="VGD55_13545"
     CDS             complement(2541..3443)
                     /locus_tag="VGD55_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0871419.1"
                     /translation="MIGTRLTDPSLVLVTGAAGRLGRNVLTACADAGIATRALILARD
                     GAAGLATDQVVIGDARDHAIVQSALAGVDAVVHLAAIPSPNAGTAEDVFCGNTAATFT
                     VLEGAARTGVRQAVIASSHAATGLPFAASVRRPAYLPIDEEIPLQVEDPYALSKQVDE
                     LTASMMWWRHGLSVIALRFPFLGDPDDRLPRRAAELAAYPALGATELWGYLDYRDAAQ
                     SCVAALTHRRPGCHVVTLAAPTTLAPYPTEQLLDAFLPHVARRTRFPGRTAPFDLTRA
                     RDLLGFAAQFGWPIDERDLHEVSA"
     gene            complement(3430..>4200)
                     /locus_tag="VGD55_13550"
     CDS             complement(3430..>4200)
                     /locus_tag="VGD55_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0871420.1"
                     /translation="FDFLGCAWDASRIHTVIDETLADHAPVGAPPTWVLSNHDVVRHV
                     TRYGRRDTSFSMDNRRLGEFSDLELGTRRARAAALVTMALPGAVYVYQGEELGLWEVE
                     DLPEALLQDPIWSRSGYTDRGRDGCRVPVPWDGDAPPFGFGRAGTEPWLPQPLGWKTL
                     TVAAETGDPDSMLELYRAALHIRRAERSLGAGDLSWLASARDVVMFGRGTDFVCIANI
                     SDQPIELPPHHAVLLASAPIEDSLLPVDAAAWLRVDRH"
BASE COUNT          723 a         1391 c         1412 g          674 t
ORIGIN      
        1 acccggcggc acgccgccgg gtcagtttgt tggtcagagc tcagctcgcc cagtcgagga
       61 gcgagtcggg gcgatcgggt tgccgaagct tggcgagcgc ctccttctcg atctgccgga
      121 tccgctcgcg ggtaagaccc acgtcgcgac cgatctcgtc gagcgtgtgc ggtctgccct
      181 cggcaagccc gaagcgcagc gcaagcaccc gcgcctcacg cggctcgagt gcagccagcg
      241 cacgatgcac cgtctcgatc atcgcctggt actccagagc gtcggacgcg cccggagcct
      301 cggagtcttc gatcaggtcg ccgagcctgg tgtcttcgtc gtcgcccacc gggttgtcga
      361 ggcttacggg ctgccgcgca ttgcgcagca tctcctcgat ctgctcaggc gtgcggtcga
      421 gctcttcagc aagctcctca gcagtgggtt cgcgaccgaa cctctggtgc atgtcgcgct
      481 ggagccggcc gagcttgttc agctgctcga cgacgtgcac agggagccgg atcgtgcggg
      541 cctggtcggc gagggctctc gtgatcgcct gccggatcca ccacatggcg tacgtcgaga
      601 acttgtagcc cttggtgtag tcgaacttct cgaccgcgcg aatcagcccg aggttgcctt
      661 cctggacgac atcgaggaac gacatgccac ggttggagta gcgcttggca accgagacga
      721 ccagtcgcag gttcgcctcg agcatgtgtg ccttggccag cgccccgtcg tccctgatgg
      781 cttcgagctc gcgccgcaac ttgggagaaa gcttgggcgt ctcgctcagc ttgtgctcgg
      841 cgtagacgcc ggcctcgatc cgcttggaca ggtcgacctc ttgggcggcg tcgagcagcg
      901 cggtgcggcc gatctcgttg aggtagttcc tgacagggtc ggccgacaca cccggctcgt
      961 cgctgtcgag gtcgggctcc tcagcgtcga cagcgtcgac cgtgtcggcg gagtcgatcg
     1021 ggtccggggt gatttcaacg cttatgtcgc ttatgccggc gtcgaccgcg agctcagcgt
     1081 cgtcgcggat gacctcaaga tcatccgtgg ctggccgagt cgccgtctgc tggcggtcac
     1141 gcgtagtgat ggcggtctcc ttcataggta ttgctctgtc acagatacaa cgcttgacgc
     1201 ttggtgatgt tcctttggtg atgttcctgt acccggtccg acgcacagat cacccaaatg
     1261 gttcgcagcc cggcacccga caggtcaggg ggcgaagacc gccccgtcgg gacgccgcag
     1321 cacgaacgag gtctcggcgg ccgccgcagc cccaagggga aaccggcgcg cggcatcctc
     1381 gtcgaggtcg atgccgaacc cgggcgccgg cgagggggtc aggtagccgc cgatggcggt
     1441 gagcgtgcca gggaacacct cgtgcacagc ctccgcaaag ttatgcgtct cttggatgcc
     1501 gaacgcgggt gaggtgacgt cgagtgcaac gttcacagcg gccccgatcg gcgagacgtc
     1561 gccgggggca tgccatgcgg tctggacgcc gaccagctcg cacatcgcgg ccagcttgcg
     1621 agccggggtc aggccgccaa ccgccgacac gtgcacgcgc aggtagtcga ccgcgtgctg
     1681 ctgcaccagc cgggccgcat cggtgaccga cgagagcagt tcgccgaccg cgatcgggac
     1741 gggcgacgcg gcgcgaacct cggcgagccg gtcgtagtgt tcgggcgcca cgacgtcttc
     1801 gaggaagaag agccgatgct cttcgagccg gcgtgcgagc acaatcgcct gcttcggcgt
     1861 gagcctgctg tgcacgtcat gaagcagctc gacgtcggcg ccgagatcgc gcctcgcccg
     1921 cgcgaacagc tctggggtcg tgcgcagata gtgcgcgacg tcccagccat cggggtacgg
     1981 cgcacccgga tacgcattcg ccgagccggg cgcgccatag gtgccgagcc caggctgccc
     2041 ggtctgcaaa cgcacatggg tccagccgcg cgcgatgatc gtcgccgcgt gctcgatcgt
     2101 ctcctcgacc gtgtgaccag cggcatgcag atacgtctga gcagcggcac ggacgcgccc
     2161 cccgaggagt tcatagacgg gcacgccgag gcgcttgcct gcgatgtccc acaacgccat
     2221 gtcgagaccc gacagcgcgt tgttcaacac cggaccgttg cgccagtacg acgagtgatg
     2281 cacggcgcgc gtcagatctt cgatgtcggc cggatgcctg ccgatgacga gaggcgcaac
     2341 acactgctcg actgctgcga cgacagcagg aaaacgctgc gtgaaggtcg cgcagccaag
     2401 gccgtacaac ccgtcgtccg acgtctcgac tttcaccacc acaagcgcga tgccctcagg
     2461 tgccgtgacg atcgcgcgta cggccgtgat gcgtaggtca tcgcgattgg cccacggctc
     2521 gacaagcagt ggagtcgcag tcacgccgag acctcgtgca gatcgcgctc gtcgatgggc
     2581 cacccgaact gcgccgcgaa tccgagcagg tcgcgagctc gggtcaggtc gaacggcgcg
     2641 gtgcggccgg gaaatcgggt tcgccgcgca acgtggggaa ggaacgcgtc aagcaactgc
     2701 tcggtcggat agggcgcgag cgtcgttggc gccgcgaggg tcacgacatg gcagccagga
     2761 cgccgatggg tgagcgccgc cacgcagctt tgcgctgcat cgcggtaatc gagatacccc
     2821 cagagttcgg tagcgcctag cgctggatag gcggcgagtt ctgcagctcg gcgcggcaac
     2881 cgatcatcgg gatcgccgag aaaggggaac cgcaacgcaa tcacgctgag cccgtgccgc
     2941 caccacatca ttgacgccgt cagctcgtcg acctgctttg acaaagcgta gggatcctcg
     3001 acctgcaaag ggatttcctc gtcgatcggc aggtaggccg gcctacggac cgaggcagcg
     3061 aacggcaacc cggttgcggc atgactgctc gcgatcacgg cctgacgtac ccccgtgcga
     3121 gctgcacctt cgagcacggt gaaggtcgcc gccgtgttgc cgcagaagac gtcttcagcg
     3181 gtgcctgcat tcggcgacgg gatcgccgcg agatgcacaa ccgcgtcaac gccggctagc
     3241 gcgctctgga cgatggcgtg gtcgcgtgcg tcgccaatca cgacctgatc ggtcgcaagc
     3301 ccggcggccc cgtcgcgcgc gaggatcaag gcccgggttg cgatgccagc gtcagcacag
     3361 gctgtcagca cgttgcgccc caggcgcccc gcggcgcccg tgacgagcac gagtgaggga
     3421 tcggtcaacc tagtgccgat caacgcgcaa ccacgcagcg gcatcgaccg gcagcagtga
     3481 atcctcgatc ggagcgcttg ccagcaacac cgcatggtgg ggcggcagtt ctatcggctg
     3541 gtctgagatg ttcgcgatac agacgaaatc ggtaccacgc ccaaacatga cgacgtcacg
     3601 agccgacgcg agccacgaca agtcccccgc cccaagcgat cgctcggctc ggcgaatgtg
     3661 caatgcggcg cggtacaact cgagcatcga gtcggggtca ccagtctcgg cggcgaccgt
     3721 gagggtcttc caaccaagag gttgcggtag ccacggctcg gtgccagctc ggccgaagcc
     3781 gaacggcggc gcatcgccgt cccacggaac cggaacccgg cacccatcac ggccgcggtc
     3841 ggtataaccg ctgcgcgacc agatcgggtc ttgcaagagc gcctctggga ggtcctcgac
     3901 ctcccagagg ccgagctcct cgccttggta gacatacacc gcgccaggga gcgccatcgt
     3961 caccaacgcc gctgcgcgtg ccctgcgcgt tccgagctcc aggtcgctga actcgccaag
     4021 gcggcgattg tccatggaga acgacgtgtc gcggcggccg taccgggtca cgtgacgcac
     4081 cacatcgtgg ttcgacaaca cccacgtagg cggcgctccg acaggtgcgt ggtcggcgag
     4141 cgtttcgtcg atcacggtgt ggatccggct cgcgtcccat gcgcagccga ggaagtcgaa
//
LOCUS       DASWVF010000764         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_21734, whole genome shotgun sequence.
ACCESSION   DASWVF010000764 DASWVF010000000
VERSION     DASWVF010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21734"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VGD55_13555"
     CDS             <1..1749
                     /locus_tag="VGD55_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEY0871421.1"
                     /translation="RCENGHLTCVASRAELGELAGRDLSELDPHRPYVDEITFACADC
                     GLQKTRVPEVIDGWYDSGALPFAQFGYPHIASEDFANSYPADFIAEAIDQTRGWFYTL
                     MAVGSLVFDQSSYRTVLCLGHILAEDGRKMSKHLGNILEPMALMDKHGADALRWYMVA
                     GGSPWSARRIGDEVLEDVVRKVLLTYWNTTSFFVLYANASNWTPGTVPAPELADRPLL
                     DRWAISELHATVEEVDSALDDFDSARAGRRLARFIDDLSNWYVRRSRRRFWDGDVSAL
                     STLHECLEVLTRLMAPFTPFVTEAVHRRIVTEVFTDLPDSVHLRDWPKVDGKLVDATL
                     GEQMTLVRRLVELGRSARAESKMRTRQPLARALVHAPGWRDLPTELRDQVIDELNVRD
                     VVSDDDVGSLAVVETVVKPNFRALGKRFGNQTQVVANAVTAADPTQLAAALTATGSAS
                     VMVDGSALELSSDELIVTEIPTLGWAVASDAGLTVALDLELNDELLGAGLAREVIRFA
                     QETRKTSGLEISDRIEVWWEADDEPLLSAMRGQRPLIAGEILAVTFTEGKPSADITPH
                     RDAELGLTMWIRVAGG"
BASE COUNT          369 a          663 c          702 g          373 t
ORIGIN      
        1 cgctgcgaga acggccacct cacctgcgtt gcatcgcgtg ccgaactcgg cgaactagcg
       61 gggcgcgatc tctccgagct tgacccgcat cggccctacg tcgacgagat cacgttcgcg
      121 tgtgccgact gcggtctgca aaagacacgg gtaccggaag tgatcgacgg gtggtacgac
      181 tcaggtgcgt tgccgttcgc gcaattcggc tacccccata ttgcgagcga agacttcgcg
      241 aattcatacc cagcagactt tatcgccgag gcgatcgacc agacgcgtgg ctggttctac
      301 acgttgatgg cggtcggcag cctcgtgttc gatcaatcga gctatcgaac cgtgttgtgc
      361 cttggtcaca tcctcgcgga agacggccgc aagatgagca agcatctcgg caacatcctc
      421 gagcccatgg cgttgatgga caagcacggt gccgatgcgt tgcggtggta catggtggcc
      481 ggcggatcgc cctggtcggc ccgtcgcatc ggcgatgagg tgctcgagga cgtcgtccgc
      541 aaagtgttgc tcacctactg gaacacaacg tctttcttcg tgctctacgc caacgcaagc
      601 aactggacgc ccggcaccgt acctgcccca gaactcgcgg atcggccgtt actcgatcgt
      661 tgggcgatct ccgaactcca cgcaacggtg gaagaagtcg acagcgcact cgacgacttc
      721 gactcggctc gtgcgggtag gcggctcgcg cggttcattg acgatctttc gaactggtac
      781 gtccggcggt cacgcagacg attctgggac ggcgatgtgt cggcgctgtc gacattgcac
      841 gagtgcctcg aggttctcac ccgcctgatg gctccgttca ctccgttcgt gaccgaagcg
      901 gtgcaccgcc gtatcgtcac cgaggtcttc accgacctgc ccgactcggt gcatctgcgc
      961 gactggccga aggttgacgg caagctggtc gacgcgacgt tgggcgagca gatgacgctg
     1021 gtgcggcggc ttgtcgagct cggtcgctcc gcgcgcgcgg aatcgaagat gcggaccagg
     1081 caaccgctgg cccgtgcgct cgtgcacgcc ccaggttggc gcgacctgcc caccgagctg
     1141 cgcgatcagg tgatcgacga gctcaacgtg cgcgacgtcg tgtccgacga cgatgtcgga
     1201 tcgctggccg tcgtcgaaac cgtggtgaaa ccgaacttcc gagcgcttgg caaacggttc
     1261 ggcaaccaga cccaggtcgt cgcgaatgcg gtgacagcag ccgacccgac gcaactcgcc
     1321 gcggcactca ccgccaccgg gtcggcgtcc gtcatggtcg atggttcggc acttgagctc
     1381 tccagcgacg agctgatcgt caccgagata ccgacgctgg gctgggccgt ggcgagcgat
     1441 gcggggctga ccgtggcgct cgatctggag ctgaacgacg agctgctcgg tgcaggtctg
     1501 gcgcgagagg tgatcaggtt cgctcaggag acccgcaaga cgagcgggct ggagatcagt
     1561 gatcgcatcg aggtctggtg ggaagccgac gacgagccgt tgctttcagc gatgcgcggc
     1621 cagcgaccgc tcatcgccgg cgagatcttg gccgtgacct tcaccgaagg taagccgagc
     1681 gccgacatca ccccccaccg cgacgccgag ctcggcctca ccatgtggat tcgggtcgcg
     1741 ggcggctgag ctgtcggctg cggccgttgc tgccggctgc gcaaacgggt gaaatctggg
     1801 caccgcacgc gtggtccgcg cgtaccaacg gccaatcacc cgatcggcgc agtcaaggtg
     1861 agggggtaca tgagccgatt gacacattgc ggcgcagatg ggttgtcggg cacgtcttag
     1921 gcaccggtcg ccgcgatcgt cggagcgtga aagggaggcg gcagatggct gcggcaccat
     1981 atttcggtca gccgatgaca gccgtggctt tgcgaccggg cgtcgtggcg gttcccgcgc
     2041 tggcgagcca agtcagaccc gccctggtgc taccggaacg cgagacccgc gagttgatcg
     2101 cctccgc
//
LOCUS       DASWVF010000765         7731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_455132, whole genome shotgun sequence.
ACCESSION   DASWVF010000765 DASWVF010000000
VERSION     DASWVF010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455132"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGD55_13560"
     CDS             complement(<1..525)
                     /locus_tag="VGD55_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0871422.1"
                     /translation="MIVVCGETLIDLVHVEGENWRALPGGSPANTAVALARLGTPAAM
                     LARISGDAFGTRLRARLVGDGVDVRYVVEASEASSMAVVDLDDSGGARYSFYLQGTAD
                     WQWSSDELPASFDASVTAIHAGSMALLMPPGGAALESMLLRERAQRVVSIDPNVRASI
                     CPDPVRYREAVERWL"
     gene            complement(522..1688)
                     /locus_tag="VGD55_13565"
     CDS             complement(522..1688)
                     /locus_tag="VGD55_13565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0871423.1"
                     /translation="MPRNPLVPRLQGLGTTIFAEMSALALETGSINLGQGFPDVDGPD
                     VIKQAAVDAIWAGRNQYPPGTGVPELRHAIVDHQRRFYGIELDADTEVLVTAGATEAV
                     AAAMLAMVDTGDEVIALEPYYDSYVASIVMAGGVRVPVTLRAPEFRLDVDRLRSAVSS
                     RTRLILLNTPHNPTGAVLTRSELEAVAEVAIEHDLIVVTDEVYEHLVYDGEHVPISTL
                     PGMSERTLTISSAGKTFAMTGWKIGWATGPAALVAACRTTKQFLTYVNGAPFQPAVAL
                     GLALDDSFYATLSGDLRRKRDLLCAGLADIGFSVFTPAGTYFVTTDVRPLGYDDGVTF
                     CRDLPKRAGVVAIPHSVFYDNVDAGRPLVRFAFCKRDEVLAEAIERLRVLGGHP"
     gene            1731..2501
                     /locus_tag="VGD55_13570"
     CDS             1731..2501
                     /locus_tag="VGD55_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0871424.1"
                     /translation="MPTTLITGSTSGIGAAFATRYAKLGHDLVLLARDRARLETQAAA
                     LHEQYDVDAEVLAADLGTDDGCAEVETRAADDRRPIDVLINNAGFGLGRDFVHSSIDD
                     EEQAVRVMIRAPMRITKAALPGMVARGNGSIITVASIAAFLNHSTYGAAKAWDVRFSQ
                     ALSVKLTGTGVRAIALCPGLVHTEFHERGKVDVSSAPRWMWIDVDQVVDACLHDLARG
                     KPICIPTRRYKVLVGVGRLLPAGVVARARNLRRPAAAR"
     gene            2513..3049
                     /gene="pyrE"
                     /locus_tag="VGD55_13575"
     CDS             2513..3049
                     /gene="pyrE"
                     /locus_tag="VGD55_13575"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0871425.1"
                     /translation="MSDRDALLREITSKAVVHGKVTLSSGREADYYVDLRRITLDATA
                     APLVGAVMLDLTENLDFDAVGGLTLGADPVAAAMLHVAASRGRKLDAFVVRKEGKAHG
                     LQRRIEGPDVAGRRVLAVEDTSTTGGSVLTAVEALREAGAEVVGVAVIVERGAAGAVA
                     DAGLEYRAAFSLSDLGLE"
     gene            complement(3084..4544)
                     /locus_tag="VGD55_13580"
     CDS             complement(3084..4544)
                     /locus_tag="VGD55_13580"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0871426.1"
                     /translation="MTTEISGFAGQVPTQLYIGGKWVGASDGSNIEVIDPSHGQVIAH
                     IANATVSDAMDAVTAASDALSDWTAAPPRDRGEILRRVFDLMTQRREELARLISIENG
                     KALPDARGEVAYAAEFFRWFSEEAVRINGSVQVAPSGANRILVLRQPVGVCVLVTPWN
                     FPAAMATRKIAPALAAGCTVVLKPATDTPLTALVLAALYEEAGVPRGVVNVVPSNRSG
                     PVVSAMLHDPRVRKLSFTGSTEVGRMLLREASDAVVNCSMELGGNAPFLVFADADLDA
                     ALEGAMVAKMRNAGEACTAANRFYVERSVAAEFSRRLAAAMGAMRVGPGLDAATQVGP
                     LVNASTRDKVASLVDDAVASGASVAVGGHAVDGPGFFYEPTVLTGVTGDAALLREEIF
                     GPVAPIVVFDDEADAVAMANDTEYGLVAYVYTSDLARGLRVSEALESGMVGLNRGLVS
                     DPAAPFGGVKQSGIGREGGHDGLLEFTESKYIAVSW"
     gene            complement(4546..5523)
                     /locus_tag="VGD55_13585"
     CDS             complement(4546..5523)
                     /locus_tag="VGD55_13585"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0871427.1"
                     /translation="MAQLSYREAVVRAIAQEMRRDESVVFLGEDIGAAGGVFKATPGL
                     LEEFGPRRVRDTPISEQAILGAAMGAAMTGLRPIAEIMFADFFAVCWDIVANEIAKSR
                     YMTNGQVSFPLVIRSGNGGGLRFGAQHSQSIENWAMAIPGLKVVAPSNAADVVGLFAA
                     AVRDPDPVLFFEQKSLYAVKSEVPDGEIVDELGKAKVLRDGDDATIVALAAMVPRALA
                     AAEELASSFGIQCTVVDVRSLVPLDTQTILREVAKTGRLFTVEENPRLCGWGAEIVSI
                     VAEEAFNDLDGPLIRITTPHIPLPAADALEDLAMPSAQRIVETVRKNLD"
     gene            complement(5511..6524)
                     /locus_tag="VGD55_13590"
     CDS             complement(5511..6524)
                     /locus_tag="VGD55_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY0871428.1"
                     /translation="MTTSTTDLAATETNPVVRLALFRMMVEMRQFEKRAYDLFMQSLV
                     KGTSHLSLGQEAIAAGFGVAMRPDDWTFATYRGHAHTLARGASMTGVMGELLGRENGL
                     MAGKGGSMHLTDVAKGVMGSYAIIGGHLPIACGAAWSAQYRGTDQVAVCFFGDGTTNI
                     GAFHEALNFAAIWKLPVVFVCENNLYMEYTPIAAVTAVPRPAADRAASYGLEAIVVDG
                     NDADVMYDIAMTALTKARAGDGPVLIEALTYRHGGHSRADPGKYRPQAEVDEWMARDP
                     IPTYRARLLDAGIDEATLADIERDVAACVDEATEAAKASPAPGVEYLMSDVWADGGSS
                     WRN"
     gene            complement(6538..>7731)
                     /locus_tag="VGD55_13595"
     CDS             complement(6538..>7731)
                     /locus_tag="VGD55_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0871429.1"
                     /translation="EDRVDFRRLRDYRLARARQALAASDLGALLLFDVNNIRYVTSTM
                     IGEWARDKMCRYALLPAGGEPVNWDFGSAARHHQLHSPWLKPENSRAGMLGLRGSVSA
                     EAGLFESAVREIKGMLTDLGLADAQIGVDIVEPPMLFEMQRQGLHVADGQQVMLDARQ
                     IKSEDEIMLLSTAAAMVDGTYFDIAEALRPGVRENELVALANKRLYDMGSDDVEAINA
                     VSGERCNPHPHNFSDRMIRPGDQAFFDIIMSFNGYRTCYYRTFAVGSATPSQRDAYTR
                     AREWIDAAIALVKPGVTTDQIARLWPAATDFGFADEMAAFGLQFGHGLGLALHERPII
                     SRLNSLEHPKEIFEGMVFALETYCPASDGFSAARIEEELVVTADGARVITLYPAQELP
                     VANPF"
BASE COUNT         1400 a         2626 c         2441 g         1264 t
ORIGIN      
        1 gagccaccgc tcgaccgcct cccggtaccg caccgggtcg gggcagatcg acgcgcgcac
       61 gttcgggtcg atcgagacca ctcgttgtgc ccgttcgcgc agcaacatcg attcgagtgc
      121 cgcgccacca ggcggcatca gcaacgccat cgagccggcg tggatcgcgg tgaccgaggc
      181 gtcgaacgag gccggcaact catccgaaga ccactgccag tcggcggtcc cttgcaagta
      241 gaagctgtag cgcgcgccgc cggaatcatc gaggtcgacc accgccatcg agctcgcttc
      301 ggacgcctcg acgacgtagc gcacgtcgac accatcacca acgagccgag cccgtaggcg
      361 agtgccgaac gcgtcgcccg agatccgcgc gagcatggcc gccggggtac cgagtcgcgc
      421 aagtgcgacc gcggtgttgg ccggcgatcc accgggcaac gcacgccaat tctcaccctc
      481 gacgtgcacg aggtcgatca acgtctcgcc acacacgacg atcacggatg acctcccaaa
      541 acccggagtc tttcgatcgc ctcggccagc acttcgtcgc gcttgcagaa cgcgaaccgt
      601 accagcggcc gacccgcatc gacgttgtca taaaacaccg agtgcgggat cgccacgaca
      661 ccggcccgct tcggcaggtc gcggcaaaac gtcaccccgt cgtcgtagcc aaggggccgg
      721 acgtcggtcg tgacgaagta ggtacctgcg ggcgtgaaca ccgagaaccc aatgtcggcg
      781 aggcccgcac acagcagatc gcgcttgcgc cgaaggtcgc cggacaacgt cgcgtagaac
      841 gagtcgtcca acgccaagcc caaggcaacc gctggctgga agggcgcgcc gttcacatag
      901 gtcaggaact gcttcgtcgt gcgacaagcc gccaccaaag cggccggacc cgtggcccag
      961 ccgatcttcc atcccgtcat cgcgaacgtc ttgcctgctg acgagatcgt caacgtgcgc
     1021 tccgacatgc cgggcagcgt cgagatcggc acgtgctcgc cgtcgtagac gaggtgctcg
     1081 tagacctcgt cggtcaccac gatgaggtcg tgctcgatcg ccacctcggc gacggcttcc
     1141 agttcgctac gggtcaacac ggcgccggtc ggattgtgcg gtgtgttcag cagaatgagt
     1201 cgcgtgcgcg agctgaccgc agagcgcaac cgatcgacgt ccagccggaa ctccggtgcc
     1261 ctcaacgtga ccggcacccg cacgccgccc gccatcacga tcgatgcgac ataggagtcg
     1321 tagtagggct cgagtgcgat gacctcatcg ccggtgtcaa ccatcgcgag catcgccgcc
     1381 gcgactgctt cggtcgcacc cgccgtgacc agcacctcgg tgtcggcgtc gagttcgatc
     1441 ccgtagaagc gtcgctgatg gtcgactatg gcgtgccgca actccgggac gccggtccct
     1501 ggcgggtact gattccgtcc ggcccagatc gcgtcgaccg cggcttgctt gatgacgtcg
     1561 ggtccgtcga cgtcgggaaa cccctgcccg agattgatcg agccggtctc aagagcaagc
     1621 gccgacatct cggcaaagat ggtggtgccg agcccctgta gtcgtggcac cagcggattg
     1681 cgaggcatgc ggtcagccta gtgatgcccc gggctggcag gctggcactc atgccgacaa
     1741 cgctgatcac cggatcgacg tcgggcatcg gcgccgcgtt cgccacccgc tacgccaagc
     1801 tcggtcacga tctggtgctc ctcgcacgcg accgtgcccg tcttgagacg caggcggccg
     1861 cactgcacga gcaatatgac gtggacgccg aggtgctggc agctgacctc ggcacagacg
     1921 acggctgtgc cgaggtagag acccgagcag ccgatgatcg ccggcccatc gacgtcctca
     1981 tcaacaacgc gggctttgga ttggggcgtg acttcgtgca ctcctcgatc gacgacgagg
     2041 agcaagcggt gcgcgtcatg atccgggcac cgatgcgcat caccaaagcc gcattgcccg
     2101 gcatggtggc gagaggcaac ggcagcatca tcaccgtcgc gagcatcgcc gcgtttctca
     2161 accacagcac ctacggtgcc gccaaagcgt gggacgtccg gttcagccaa gccctgtcgg
     2221 tgaagctcac cgggactggt gtgcgggcga tcgcgctttg cccgggtctt gtgcataccg
     2281 agttccacga gcgcggcaag gtcgacgtct cgagcgcgcc gcgctggatg tggatcgacg
     2341 tcgaccaggt ggtcgacgcc tgcctgcacg acctcgctcg gggcaagccg atctgcatcc
     2401 cgacccggcg ctacaaggtg ctggttggcg tcgggcggct gcttccggcg ggcgtggtgg
     2461 cccgtgcccg caatctgcgg cgacctgctg cggcacgtta ggctcatgcg ccatgagcga
     2521 ccgtgacgca ctcctgcgcg agatcaccag caaggccgtc gtgcacggca aggtgaccct
     2581 gagctcgggc cgcgaggccg actactacgt cgatctgcgc cggatcaccc tcgacgcaac
     2641 cgccgcaccc ctcgtcggcg ccgtgatgct cgacttgact gagaacctcg acttcgacgc
     2701 cgtcggcggt ctcacgctcg gcgccgaccc ggtggcagca gcgatgctgc acgtagccgc
     2761 cagccgcgga cgcaagctcg acgcattcgt cgtccggaag gaaggaaaag cgcacggtct
     2821 tcagcgtcgc atcgaagggc ctgatgtagc cggacgccga gtgctcgccg tcgaagacac
     2881 ctccaccacg ggcgggtcgg ttctcacggc agtggaggcg ttgcgcgagg cgggcgcaga
     2941 ggttgtcggc gtcgccgtca tcgtcgaacg cggcgcggcc ggtgccgtcg ccgacgcggg
     3001 cctggaatat cgcgcggcat tttcgctgtc cgacctcggt ctcgaatagc cccgagcgtc
     3061 gagcacccgc aggcttggct tggtcaccac gaaacggcga tgtacttgct ctcagtgaac
     3121 tccaacaagc cgtcgtgccc gccctcacgg ccgatgccgc tttgcttgac gccgccgaac
     3181 ggtgcggcgg gatcggacac gagaccgcgg ttgagcccga ccatgccgga ttcgagcgct
     3241 tccgacacac ggagcccgcg cgccaaatcg ctcgtataga cgtacgcgac gagcccgtac
     3301 tcggtgtcat tggccatcgc gaccgcgtcg gcctcgtcgt cgaagacgac gatcggggcc
     3361 accggcccga agatctcctc acgcaagagc gctgcgtcgc cggtcacgcc ggtgagcacg
     3421 gtcggctcgt aaaagaaccc cggaccgtcg acggcatggc cgccaaccgc gacactcgca
     3481 ccactggcga ccgcgtcatc gaccaatgac gccaccttgt cgcgcgtcga ggcattgacc
     3541 agcggaccta cctgtgtcgc agcatcgagc cccgggccaa cgcgcatcgc acccatcgca
     3601 gcagcgagac ggcggctgaa ctcggcggcc accgagcgct cgacgtagaa acgattcgcg
     3661 gcagtgcaag cttcgccggc gtttcgcatc ttggcgacca tggcgccttc gagtgccgcg
     3721 tcgagatcgg cgtcggcgaa caccaggaac ggggcattgc cgccgagttc catcgagcag
     3781 ttcaccacgg cgtccgacgc ctcgcgcagc agcatgcgtc cgacctcggt cgatccggtg
     3841 aagctcagct tgcgaacacg agggtcgtgc aacatcgccg acaccacagg tccagaacga
     3901 ttcgagggga cgacgttcac cacgccccgc gggacgcccg cttcttcgta caaagccgct
     3961 agcacgaggg ctgtcaacgg cgtgtcggta gccggcttca acacgaccgt gcagccggcg
     4021 gcaagagcgg gagcgatctt gcgagttgcc atggcagccg ggaagttcca cggtgtgacg
     4081 agaacacaga cgccgaccgg ctggcgcagc acgagaatgc gattcgctcc ggaaggcgcg
     4141 acctgaacgc tgccgttgat gcgcacggct tcttcggaga accagcgaaa gaactccgcg
     4201 gcgtacgcga cttcgccccg tgcgtcaggg agcgccttgc cgttctcgat cgaaatcagc
     4261 cgcgcgagtt cctcgcgccg ctgcgtcatc aaatcgaaca cgcggcgcaa gatttcgcct
     4321 cgatcgcggg gtggtgccgc cgtccagtca gacaatgcgt cggacgccgc ggtgacggcg
     4381 tccatggcat cgctcacagt cgcgttggca atgtgagcta tcacctggcc gtgcgacgga
     4441 tcgatgacct cgatgttcga cccgtcggac gccccgaccc acttgccgcc gatgtatagc
     4501 tgggtcggca cctgccccgc aaacccggag atctcggtgg tcatgtcagt cgaggttctt
     4561 gcggacggtc tcgacgatgc gttgcgcgct cggcatcgcc aggtcttcca acgcgtcggc
     4621 cgccggcagc gggatgtgcg gcgtcgtgat gcggatcagc gggccgtcga gatcgttgaa
     4681 ggcctcctcg gcgacgatcg agacgatctc ggcaccccag ccgcacagcc gcggattctc
     4741 ttcgacagta aacaggcggc ccgtcttcgc tacctcacga aggatcgtct gggtatcgag
     4801 cggcaccaag ctgcgcacgt cgacgacggt gcattgaatg ccaaaggagg acgcgagttc
     4861 ttcggcggca gcaagggcgc gcggaaccat tgcggcgaga gcgacgatag tggcgtcgtc
     4921 accgtcgcga agtaccttcg ccttacccaa ctcgtcaact atctcgccgt ccggcacctc
     4981 gctcttaact gcgtacagcg acttctgttc gaagaacagc accggatctg ggtcgcgcac
     5041 cgcggcggcg aagagtccga caacatcagc cgcgtttgac ggcgccacga ccttgagccc
     5101 tgggattgcc attgcccagt tctcgatcga ctgcgagtgc tgcgcgccga aacgcaagcc
     5161 cccaccgttg cctgaacgga tgacaagggg gaacgagacc tggccgttcg tcatgtagcg
     5221 actcttggcg atctcgttgg cgacgatgtc ccagcacaca gcaaagaagt cggcgaacat
     5281 gatctcggcg atcggtcgca agcccgtcat cgcggcgccc attgctgcgc cgagaatcgc
     5341 ctgctcgctg atgggggtgt cacgcacccg gcgtggaccg aactcctcga gcaagccggg
     5401 tgttgccttg aacacgccgc ccgctgcacc gatatcttcg ccgagaaaga cgacggactc
     5461 gtcacgccgc atctcctgcg cgattgcgcg cacgacggcc tcgcgataac tcagttgcgc
     5521 cacgacgaac ctccgtcagc ccacacgtca ctcatcaagt actcgacccc cggcgccggt
     5581 gatgctttgg ctgcctcggt agcttcgtcg acgcaagcag caacgtcgcg ctcgatatcg
     5641 gcgagcgtcg cttcatcaat gccggcatcg agaagacgcg cgcgataggt cggaatcggg
     5701 tcgcgcgcca tccactcgtc gacttccgcc tgcggccggt acttgccggg gtcagcgcgg
     5761 gagtgcccgc cgtgccggta ggtcagtgct tcgatgagca ccggaccatc accggcgcga
     5821 gccttggtca gggccgtcat cgcgatgtcg tacatgacgt cggcgtcgtt tccgtcgacg
     5881 acgatcgcct cgagcccgta cgacgccgcg cgatccgccg ccggtcgggg gactgccgtc
     5941 accgcggcga tcggggtgta ctccatgtac aggttgttct cgcacacgaa caccaccggc
     6001 agcttccaga tcgcggcgaa gttcaacgcc tcatggaacg caccgatgtt ggtcgttccg
     6061 tcaccgaaga agcacacagc gacctgatcg gtgccgcggt actgggccga ccacgccgcg
     6121 ccgcacgcaa tgggcaggtg cccaccgata atcgcgtagc tccccatgac gcctttcgcg
     6181 acgtcggtga ggtgcatcga gccacccttg cccgccatca agccgttctc gcgaccaagc
     6241 aactcgccca tgacgccggt catcgacgct ccgcgcgcga gggtatgcgc gtgccctctg
     6301 tacgtggcga acgtccagtc gtcggggcgc atcgcgacac cgaaccccgc tgcgatcgcc
     6361 tcctgtccaa gtgacaaatg gctggtcccc ttcacgagac tttgcatgaa caagtcgtac
     6421 gcgcgcttct cgaactggcg catctcgacc atcatgcgaa agagcgcgag ccggacgacg
     6481 gggttggttt cggtcgcggc taggtcggtc gtgctggtgg tcatgtcggt gcccctctta
     6541 aaacggattg gcgacaggaa gctcctgcgc cgggtacagc gtgatgacgc gcgcgccatc
     6601 ggcggtcacg acaagctctt cctcgatccg cgctgcggag aaaccgtcgc ttgccgggca
     6661 gtaggtctcg agcgcgaaca ccatgccttc gaagatctct ttcggatgtt cgagcgagtt
     6721 caatcggctg atgatcggcc gctcgtgcaa ggccaagccc aacccgtgcc cgaactgcaa
     6781 accaaacgcc gccatctcgt cggcaaagcc gaagtcggtg gcagcgggcc acagtcgcgc
     6841 aatctggtcg gtcgtaacgc ccggcttcac cagcgcaatg gcggcatcga tccattcgcg
     6901 cgcccgcgtg taggcgtcgc gctgcgacgg agtcgcgcta ccgaccgcga acgtgcggta
     6961 gtagcaggtg cggtatccgt tgaacgacat gatgatgtcg aagaacgcct ggtcgccggg
     7021 tcggatcatc cggtcggaaa agttgtgcgg atgcgggtta caccgctcgc ccgacaccgc
     7081 gttgatcgct tcgacatcgt cagagcccat gtcgtagagc cgcttgttcg ccagcgcgac
     7141 gagctcgttc tcgcgcaccc cgggacgcaa ggcctcggca atgtcgaagt aggtaccgtc
     7201 gaccatagcc gcggcggtcg acagcaacat aatctcgtct tcgctcttga tctgccgagc
     7261 atcgagcatg acctgctggc catcggcgac gtgcaggcct tgacgttgca tctcgaacag
     7321 catcggcggc tcgacgatgt cgacgccgat ctgcgcatct gccaacccga gatcggtcag
     7381 catgcccttg atctcgcgca ctgccgactc gaacagccct gcctctgccg acaccgagcc
     7441 acgcagaccc aacattccgg cccgtgagtt ctctggcttg agccacggcg agtgcagttg
     7501 atggtgccgc gcagcggagc cgaagtccca gttcaccggc tcgccgccag ccggtagcag
     7561 tgcgtagcga cacatcttgt cgcgcgccca ctcgccgatc atcgtcgacg tgacgtacct
     7621 gatgttgttg acgtcgaaca gcaggagagc accaaggtcg ctcgcggcca gcgcctgccg
     7681 ggcgcgcgcg aggcgatagt cacgcagccg gcggaagtcg acccggtctt c
//
LOCUS       DASWVF010000766         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_301666, whole genome shotgun sequence.
ACCESSION   DASWVF010000766 DASWVF010000000
VERSION     DASWVF010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301666"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VGD55_13600"
     CDS             <1..1532
                     /locus_tag="VGD55_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0871430.1"
                     /translation="PSWVLLGIPVVATLCTVALLVYDHGHMLHPIAVALAAATIVAAI
                     GRLVVSFREVTTLAHSRQLALTDELTGLGNRRAFYEHVDTYLAADPDRSGALFLLDLD
                     RFKEVNDSLGHHAGDDLLLQVATRLTEGLTHEDGLLARLGGDEFSIFVLDVDRDGAEM
                     FAEMVRQTLLAPFTIDGITVRVAASVGISLFPKHGLEVSTLLRRADIAMYHAKDLRTG
                     FRVYSESDDSLGGQDRLRTLEELRQVIQARKLAMHYQPKVDSQTSYVSGVEALVRWDH
                     PVHGLLPPDAFLPLAEDAGLMRDLTMAVLEQSLDQVAAWRAGGRMLSVAVNLSASSLV
                     DLELPERVHQLLVNRGLPASALELEITEDFLMGDRERAREILTQLRTLGIRVAVDDFG
                     TGYSSLAYLRELPIDELKLDRSFVTPMADDPRAAAIVRSTISLAHSLGMRLVAEGVEN
                     EITAGHLALSGCDVSQGFFFSKALPPLELERWLDDRPDMAGMEATAVQVGPRATDGLA
                     S"
     gene            1529..>1800
                     /locus_tag="VGD55_13605"
     CDS             1529..>1800
                     /locus_tag="VGD55_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871431.1"
                     /translation="MTAAPLMAASTDLLSGTTGVQIVSGLHGVREHRGELEALAQRCG
                     AAITARSTWTLANAGSGQPWAVLVRDPSGFLRAASVLVEVRGAGRTS"
BASE COUNT          280 a          624 c          566 g          330 t
ORIGIN      
        1 tcccctcatg ggtattgctc ggcatccccg tcgtagccac gctgtgcacg gtcgctctgc
       61 tggtgtacga ccacggacac atgctgcacc ccatcgccgt tgcgctggct gcagcaacga
      121 tcgtggccgc gatcggccga ctcgtcgtca gcttccgcga agtcacgacc ctggcgcaca
      181 gccggcagct cgccctcacc gacgagctga ctggccttgg caaccggcga gcgttctacg
      241 aacacgtcga cacctacctt gcggcagacc ctgaccgcag tggcgcgctg ttcctgctcg
      301 accttgaccg cttcaaggaa gtcaacgact cactcggcca tcacgccggc gacgacctgc
      361 tgctgcaggt tgccacccga ctcaccgagg ggctcactca cgaagacggg ttgctcgcgc
      421 gcctcggcgg cgacgagttc tcgattttcg tattggacgt cgaccgcgac ggcgccgaga
      481 tgttcgccga gatggtccgc cagacgctgc ttgcgccatt caccatcgac ggcatcaccg
      541 tgcgggtggc tgcgagtgtg ggcatctcgc tgttccccaa gcacggcctc gaagtgtcaa
      601 ccctgctgcg ccgagccgac atcgcgatgt accacgctaa agacctgcgc accgggttcc
      661 gcgtctacag cgagtctgac gactcactgg gtggacagga ccgcctgcgc actctcgagg
      721 aactgcgcca ggtgattcag gcccgcaagc tggccatgca ctaccaaccg aaggtcgatt
      781 cgcagacctc gtacgtgtcg ggggtcgagg ctctcgtccg ctgggatcac cccgttcacg
      841 ggttgctccc acccgacgcg ttcctgccgc tcgccgagga cgcaggactc atgcgtgacc
      901 tcacgatggc cgtgctcgag cagtcgctcg accaggtcgc ggcgtggcgg gctggtggcc
      961 ggatgctcag tgtggccgtc aacctctcgg cttcgtcgtt ggttgacctc gaactccccg
     1021 agcgggtgca ccagctgctg gtgaaccgcg ggctgccagc gtcggctctc gagctcgaaa
     1081 tcaccgagga cttcctgatg ggtgaccggg aaagggcccg cgagatcctg acgcagttgc
     1141 gcaccctcgg tatccgggta gcggtcgacg acttcggcac cggttactcg tcgctcgcct
     1201 acttgcgcga gctgccgatt gacgagctga agcttgaccg ttcgttcgtc acgccgatgg
     1261 ctgacgatcc gcgggctgcg gcaatcgtgc gctcaacgat cagcctggcc cactcgctgg
     1321 gtatgcgtct tgtcgccgaa ggggtcgaga acgagatcac cgccgggcac ctcgcgctct
     1381 ccggatgcga cgtctcgcag ggattcttct tctccaaggc gcttccgccg ctcgagctcg
     1441 aacggtggct cgacgaccgg ccggatatgg caggcatgga agccactgcc gttcaggtcg
     1501 gtccccgcgc gacggacgga ctggcctcgt gaccgctgcg ccactcatgg cagccagcac
     1561 ggatctgctt tccggaacca cgggggttca gatcgtctcg ggcctccacg gcgtgcgcga
     1621 gcatcgtggc gagctcgaag cgttggctca acgctgcggc gctgccatca cagctcgtag
     1681 cacctggacg ctcgccaacg ccggcagtgg ccaaccatgg gcagtgctcg tccgtgaccc
     1741 gtccggattc ttgcgtgctg cgtcggtcct cgtcgaggtt cgcggcgccg gccgcacctc
//
LOCUS       DASWVF010000767         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_98132, whole genome shotgun sequence.
ACCESSION   DASWVF010000767 DASWVF010000000
VERSION     DASWVF010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98132"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1947
                     /locus_tag="VGD55_13610"
     CDS             <1..>1947
                     /locus_tag="VGD55_13610"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0871432.1"
                     /translation="FSSPANNLVVAFDGTKSTDSDGTIASYAWTFGDGSTGTGAKPTH
                     TYATGGKYNVQLTVTDNDGATNAITQPVTVAAANIPPTAAFSSTSKNYTATFDASGSK
                     DTDGTIASYAWNFGDNTTGTGVNPSHTYAAGNTYLVTLTVTDNQGATGTVAQSVTVSN
                     APPTAAFTSSAAGLAASFNAGASADSDGTVASYAWTFGDGSTGTGVTPTHTYATGGTY
                     SVNLIVTDNQGATGTVTNPITVAQGAVTPFATDSFGRTVTGGWGSADSGGAYTVTGGA
                     SNFSVGSGVGTMKLATAGSSPSAALASVSSTDTEVRVGVALNEAQTGGGTYVSVVGRR
                     ISATTDYRVKLKYASTGVVTEVLEQVTGSTETVLKSIVVPGVTYTPGTVLDVRLQVTG
                     TSPTTLNSMVWAAGTTQPTAWQATATDSTAGLQVAGGVGLITYLSSSATNAPENASFN
                     TLWAGPTHASGVNIPPTASFSSSSSALVASFNAGGSSDSDGTIASYAWNFGDGSTGTG
                     VTPTHTYATGNTYSVQLTVTDNQGATGTVTNPITVVAAGGITPFVTDSFGRTVASGWG
                     SADTGGVYTISGGSSNFSVGAGVGNIKLAAAGSSASAALATVSSTDTEVRVGVTLNEA
                     QTGGGTYVSVIGRRISSTSDYRVK"
BASE COUNT          372 a          680 c          604 g          291 t
ORIGIN      
        1 cattcagctc gccggcgaac aacctcgtgg tcgccttcga cggcaccaag tcgactgaca
       61 gcgacggcac gattgcttcc tacgcctgga ccttcggtga cggctcgacc ggcactggcg
      121 cgaagccgac ccacacgtac gccacgggcg gcaagtacaa cgtccagttg accgtcaccg
      181 acaacgacgg tgcaacgaac gcgatcaccc agcccgtcac ggtcgccgcg gcgaacatac
      241 cgccgaccgc ggcgttctcg tcgacgtcga agaattacac cgcaacgttc gacgcaagtg
      301 gatcgaagga caccgacggc acgatcgcgt cgtacgcatg gaacttcggt gacaacacaa
      361 caggcactgg cgtgaacccg agccacacct acgcggccgg caacacctac ctcgtcacgt
      421 tgacggtgac cgacaaccag ggcgctacgg gtacggtcgc tcaaagcgtc accgtctcca
      481 acgcgccacc gaccgcagca ttcacgtcgt ccgctgctgg attggcggcg agcttcaacg
      541 caggcgcgtc ggccgacagc gacggaacgg tcgcgtcgta tgcctggacg ttcggtgacg
      601 gctcgaccgg caccggcgtc acaccgacgc acacgtacgc aacgggtggc acgtacagcg
      661 tcaacctgat cgtcaccgac aaccagggcg cgacaggcac tgtcaccaac ccgatcacgg
      721 ttgctcaagg tgcggtcacg ccgttcgcga ccgactcgtt cggccgcaca gtgaccggcg
      781 gttggggcag cgccgacagc ggcggtgcgt acacggtcac cggtggggct agcaacttct
      841 ccgtcggtag cggtgtcggc accatgaagc tggcgaccgc aggcagcagt ccgtcggccg
      901 ctctcgcgag tgtgtcgtcg accgacaccg aagtgcgcgt cggcgtggca ctcaacgagg
      961 cacagaccgg cggtggcacc tacgtctccg tggtcggccg tcgcatcagt gcgaccaccg
     1021 actaccgggt caagctcaag tacgcctcga ccggtgtggt caccgaggtt ctcgagcagg
     1081 tcaccggcag caccgaaacc gtgctgaagt cgatcgtcgt tcctggcgtt acttacacgc
     1141 ccggcacggt gctcgacgtg cggctacagg taactggcac ctcgccgacg acactgaact
     1201 cgatggtctg ggcagctggc acaacccagc cgaccgcgtg gcaggcaact gccaccgaca
     1261 gcaccgctgg cctacaggtc gccggtggcg tcgggctcat cacctacctg tcgagcagcg
     1321 cgaccaacgc tcccgagaac gcatcgttca acacgctctg ggccggtccg acccacgcga
     1381 gcggcgtcaa cattccgccg accgcatcgt tcagctcgtc gtcgtccgcg ttggttgcaa
     1441 gcttcaatgc aggcggctca agtgacagcg acggaaccat cgcctcctac gcgtggaact
     1501 tcggcgacgg ctcaacgggt accggcgtca cgccgacgca cacgtacgcc acgggcaaca
     1561 cctacagcgt gcagttgacc gtcactgaca accagggcgc gaccggcact gtgacgaacc
     1621 cgatcacggt cgtcgcggca ggcggcatca ccccgttcgt gaccgactcg ttcggccgca
     1681 cggttgccag tggctggggc agcgctgaca ccggcggcgt ctacacgatc tcgggaggtt
     1741 cttcgaactt ctcggtcgga gccggcgtcg gcaacatcaa gctggcagcc gctggcagca
     1801 gcgcctccgc agctctcgcg accgtgtcgt cgaccgacac cgaggtgcgg gtcggcgtca
     1861 cgctgaacga ggcacagacc ggcggtggta cgtacgtctc ggtcatcggc cggcggatca
     1921 gctcgaccag tgactaccgc gtcaagc
//
LOCUS       DASWVF010000768         5053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_251008, whole genome shotgun sequence.
ACCESSION   DASWVF010000768 DASWVF010000000
VERSION     DASWVF010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251008"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="miaB"
                     /locus_tag="VGD55_13615"
     CDS             <1..1212
                     /gene="miaB"
                     /locus_tag="VGD55_13615"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY0871433.1"
                     /translation="GMQIAVGGCLAQKDRGEIVRRAPWVDVVFGTHNVGSLPVLLERA
                     RIAEEAQIEILESLDVFPSTLPSRRESVYAAWVAISVGCNNSCTFCIVPSLRGTEKDR
                     RPGDILLEIETLVADGVIEVTLLGQNVNSYGVEFGDKQAFSKLLRACGSIDGLERVRF
                     TSPHPRDFTDDVIDAMATTPNVMHQLHMPLQSGSDEVLRRMRRSYRRDKYLGIIERVR
                     SAMPDAAITTDVIIGFPGETDEDFEHTLDVVRLARFANCYTFQYSKRPGTPAADMDDQ
                     VSPSVVRERYERLVAVADEVAWDEAKTQVGRVVDVLIAEGEGRKDDQTHRMSGRAADN
                     RLVHLATTDARPGDMASVEVTYAAPHHLVADIVRGVRRTRAGDAWEARQGSTQRQQVG
                     VPLGMPAMPAR"
     gene            1257..2228
                     /locus_tag="VGD55_13620"
     CDS             1257..2228
                     /locus_tag="VGD55_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871434.1"
                     /translation="MRLRVSLLVALSLAAASCASRTAVSSLSGVSSLSSVVASTSSVA
                     APYCVDPNPATPKAVSPAIPNVAADGRLTSTWSLDSGQATVVPADGAQPRVDRQQALC
                     TLLAASEINGLDVLEDDTGFSLVLGKVSIADELLASQVPGATDVGAQSPPPLTSFHSR
                     LAWVGVIDPPVRSSCGMDGFASTTPSPTLVPYQLLILDADTGADGIVYEARTYMPCVG
                     SLSFGPEVAPLTVNVSVPWRLISRDPGGMFGTIAVTVTACDSYGDGTNTSSSQVGLLE
                     VDVERPIGACGPPKDVTQTLRGPTVSDPLPATLTHAAVGYRDAAPDS"
     gene            2335..3384
                     /locus_tag="VGD55_13625"
     CDS             2335..3384
                     /locus_tag="VGD55_13625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0871435.1"
                     /translation="MTTLESLPLSVLDLAPVPTGSSVGAALRSTIALAQRAEQLGYKR
                     FWVAEHHNMPGIASSSPAVLIGHIAAATSTLRVGSGGVMLPNHSSLVVAEQFGMLDAL
                     HPGRIDLGIGRAPGSDQLTALALRRSVEALSADDFPSQLTNLMSYFSAEWPAGHPFAA
                     ITAVPGAGAHPAVWLLGSSDYSAQVAAMLGLPFAFAHHFSPANTLPAVALYRRLFEPS
                     ATLESPYTMIGVSVTCAETDAEAQRLAAPAALSFLRLRTGRPSTLPSPEEAAGYPYTP
                     EERAFVDGRRASQIIGSPETVRTGLTDLLEATAADEFMVTTTTFDPTDRLRSFELVAD
                     IANLSSREMAAGPAA"
     gene            complement(3426..3794)
                     /locus_tag="VGD55_13630"
     CDS             complement(3426..3794)
                     /locus_tag="VGD55_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871436.1"
                     /translation="MTGHDHSHGGEHHTHDHRHEAGGGPTVLDIGDDVGALVLYTSLE
                     LVGAEIEISPDGEPDRRRHVAVHPRQYPGGTAYAAVYYGLEAGSYQLWAADGSVALTV
                     SIEGNTITEAMWPEGALAVR"
     gene            complement(3791..>5053)
                     /locus_tag="VGD55_13635"
     CDS             complement(3791..>5053)
                     /locus_tag="VGD55_13635"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY0871437.1"
                     /translation="IANYVPLQAPDGGPNFFEFGDDVLYEIHISNRGDAQADITYGFR
                     FHTVVFNKNTFLYNTGQITSIFDSHFNRRQYYTVTRTTRNGSTKVLGSNLEVPPCNIG
                     PRSTPNYEHLAAQCVHGLAGNRTVFAGQRADAFHVDLGSVFDLLDLRPFQNLHLISTA
                     ATTGINSLANSNVHSIALRVPFSDLTVDGKTPTDPANLKSVVGIWTTASRQRTRLYEG
                     PGANAQTFGGWEQVSRLGNPLINEVVIPMGSKDDWNHSLPSNDSDYAASFLHPEVAGL
                     LPVLYPGVFPHLAAYTKPRADLVAILGTGIPSGLIPGFQNYTGKTVADMLRLNLAIPA
                     TPADKVSAAGLAGGDAAGFPNGRRVGDDVVTIELRAIAGLLIPLVDKSFVPDGATSLI
                     DDGSTGAPTQNVFPYLNSPHDGYDVGVA"
BASE COUNT          851 a         1600 c         1694 g          908 t
ORIGIN      
        1 ggcatgcaga tcgcggtcgg cgggtgcctt gcgcagaagg atcgcggtga gatcgtccgg
       61 cgagcgccgt gggtcgacgt tgtcttcggt acccacaacg ttgggtcgct gccggtgttg
      121 cttgagcgag cgcgaatcgc cgaggaagcg cagatcgaga tcctcgaatc gctcgacgtg
      181 tttccctcga cgctgccatc gcgtcgtgaa tcggtgtatg ccgcgtgggt tgccatcagc
      241 gtcgggtgca acaacagctg cacgttctgc atcgtgccaa gtttgcgggg caccgagaag
      301 gacaggcgac ctggcgacat ccttctcgag atcgagacac ttgtcgccga tggcgtcatc
      361 gaggtgacat tgctcggcca gaatgtgaac tcgtacggag tcgaattcgg cgacaagcaa
      421 gcgttttcta agctcctgcg agcctgcggt tctatcgacg gactcgagcg ggtgcgattc
      481 acctcacccc acccgcgcga tttcaccgac gacgtgatcg atgcgatggc gacaaccccg
      541 aacgtaatgc accagctgca catgccgttg cagtcaggct ccgacgaagt gttgcgccgc
      601 atgcggcggt cgtaccggcg cgacaaatac ctcggcatca tcgagcgggt ccgcagcgcg
      661 atgccggacg ccgccatcac cacggacgtc atcatcggat tccccgggga gacggacgaa
      721 gatttcgagc acaccctgga cgtcgtgcgg ctggcgcgtt ttgccaactg ctacaccttc
      781 cagtactcga agagacctgg cacgccggcg gccgatatgg acgatcaagt gtcgccatcg
      841 gtggtgcgtg aaaggtacga gcggctggtc gcagttgccg acgaggtcgc ctgggacgaa
      901 gcgaagaccc aggtcgggcg cgtcgtcgac gtgctcatcg ctgagggcga aggtcgcaag
      961 gacgatcaga cccatcgcat gagcggccgg gccgccgaca atcgcctggt gcacctcgcc
     1021 actaccgacg ctcggccggg ggatatggcg agcgtcgagg tcacgtacgc ggcccctcac
     1081 cacctggtgg ccgacatcgt ccgcggggtg cgccgcacga gggctggcga tgcgtgggaa
     1141 gcgcggcaag gatcgacgca acgccagcag gtcggcgtcc cgctaggaat gcctgcgatg
     1201 cctgcccgct gagcggaacc aagaggtgta gcaccgagcg tcggaggggg gtgagcatgc
     1261 ggctgcgagt cagtctgctg gtcgcgcttt cgctggcggc cgccagctgc gcaagccgaa
     1321 cggcggtctc gtccttgagt ggggtctcgt cattgagctc ggtcgtggct tcgacgtcct
     1381 cggtggccgc gccgtattgc gtggacccga acccagctac accgaaggca gtgtcgccag
     1441 cgatccccaa cgtcgcagcc gacggcaggc tcacgtcgac gtggtcactt gacagtggcc
     1501 aggcgaccgt ggtgcccgcg gatggtgcgc agccccgagt cgatcgccag caggccctct
     1561 gtacgttgct ggctgcgagc gagatcaacg gacttgatgt ccttgaagac gacaccggct
     1621 ttagtttggt cttgggcaag gtcagcatcg ccgacgagct gctggcctca caggtacccg
     1681 gtgcgaccga tgtcggcgca cagtcgccgc cgcccttgac ctcgttccac tcgcggcttg
     1741 cttgggtcgg cgtgatcgat ccgccggtga ggtcgtcgtg cggcatggac gggtttgcct
     1801 cgaccacacc gagccccaca cttgtgccgt accaactgct tattctcgac gccgacacgg
     1861 gagctgacgg catcgtctac gaagcgcgca cgtatatgcc gtgcgtcggg agcttgtcct
     1921 tcggtccgga agtcgcgccc cttacggtga acgtctcagt gccgtggcgg ctgatctcac
     1981 gtgatccggg tggcatgttc gggacgatcg ccgtcacggt gacggcatgc gacagctatg
     2041 gcgacggcac aaacacgtct agctcgcagg tgggtctgtt ggaggtcgat gtcgagcgac
     2101 cgatcggcgc atgcggccct ccgaaggacg tcactcaaac cctgcgcgga ccgactgtga
     2161 gtgatccgct gccggccacg ttgacccacg ccgccgtcgg ctaccgcgac gctgctcctg
     2221 acagttgatg gcccggacgg cgtcagacga cgggtgtgct ctgctggacg ccaagaccgc
     2281 ccaccgggtt gcacgcgcag gaagaacgcg cccgtaccac cggttgggac tgatgtgacc
     2341 accctcgaga gccttccgct gtctgtgctc gacctcgcgc ccgtcccgac cggatcgagc
     2401 gtcggcgctg cactccgcag cacgatcgcg ctcgcacagc gagccgaaca gcttggctac
     2461 aagcgtttct gggtggcaga gcaccacaac atgccgggga tcgcgagttc gtcgccggcc
     2521 gtgctcatcg ggcacatcgc ggcggcgaca tctaccttgc gtgtcgggtc cggcggcgtc
     2581 atgctgccga accattcgtc gctcgtcgtt gccgagcagt tcggaatgct cgatgcactg
     2641 cacccgggga ggatcgatct cggcatcggg cgtgcgcctg gcagcgacca actgacggca
     2701 ctcgctctaa ggcgatcagt tgaggccctc agtgctgacg acttcccttc gcaactgacc
     2761 aatctgatgt cgtacttcag tgccgaatgg ccggccgggc accccttcgc cgcgatcacc
     2821 gccgtgcccg gtgctggcgc gcatcccgcc gtctggttgc tgggatcgag tgactacagc
     2881 gcgcaggttg ccgccatgct cgggttgccg ttcgcgttcg cccaccactt cagcccagcc
     2941 aatacgcttc ccgcagttgc cttgtatcgc agacttttcg aaccgtcagc aaccctcgag
     3001 agtccgtaca cgatgatcgg cgtcagcgtc acctgcgccg agacggacgc cgaggcgcaa
     3061 cgtctcgcgg cccccgcggc cttgtcgttc ctgcggctca gaacagggcg gccgtcgaca
     3121 cttccctcgc ccgaagaggc agccgggtac ccgtacacgc ctgaggagcg cgcgttcgtc
     3181 gacggccggc gcgcgtcgca gatcatcggc tcgcccgaga cggttcgcac gggtctaacc
     3241 gacttgctcg aggcgaccgc cgcagatgag ttcatggtca ccacaacgac cttcgatccg
     3301 accgatcggc tcaggtcgtt cgagctcgtg gccgacatcg cgaacctgtc ctcgcgtgaa
     3361 atggcagcgg ggccggccgc ttgatagcga ccgaccccgc tgttcccccc aactggccgt
     3421 aaggcttagc gaactgcaag agctccttcg ggccacattg cttcggtaat cgtgttgcct
     3481 tcgatcgaca cggtcagtgc gaccgaacca tcggcggccc acagctggta gctgcctgcc
     3541 tcgaggccgt agtacacggc tgcgtacgcc gtcccgcccg ggtactggcg agggtgcacg
     3601 gccacgtgcc gccgacggtc aggttcgccg tccggactga tctcgatctc ggctccgacc
     3661 aactcgagcg aggtgtagag caccagtgcg ccgacgtcgt caccgatgtc gagcactgtc
     3721 ggtcccccgc ccgcctcgtg acgatggtca tgagtgtggt gctcgccccc gtgcgagtga
     3781 tcgtggccgg tcatgcgacg ccgacgtcgt atccgtcgtg gggcgagttc aggtacggga
     3841 acacgttctg cgtcggggca cccgtcgatc cgtcgtcgat caggctggtg gcaccgtcgg
     3901 gcacgaacga cttgtcgacc agcgggatca gcagacccgc aatcgcgcga agttcgatcg
     3961 tgaccacgtc gtcgcccacg cgccggccgt tcgggaaccc ggctgcgtcg ccgcccgcga
     4021 gacctgccgc actgaccttg tcggccggtg ttgccgggat cgccaggttg agacgcagca
     4081 tgtccgccac ggtcttgccc gtgtagttct ggaacccggg gatgaggccg gacgggatgc
     4141 cggtacccag gatcgcaacc aggtctgcac gaggcttcgt gtacgcggcc aagtgcggga
     4201 acacgccggg gtagaggacc ggaagcaggc cagcgacctc ggggtgcagg aacgaagcgg
     4261 cgtagtcgga gtcgttcgaa ggaaggctgt ggttccagtc gtccttcgaa cccatcggga
     4321 tgacaacttc gttgatgagc gggttgccca gacgggatac ctgctcccag ccaccgaagg
     4381 tctgcgcgtt ggcgcccggg ccctcgtaaa ggcgggtgcg ctgacggctt gccgtcgtcc
     4441 agatgccgac gacagacttc aggttcgccg ggtcggtggg cgtcttgccg tcaacagtca
     4501 ggtcgctgaa cggaacgcgc aacgcgatgc tgtgaacgtt gctgttggcg agtgagttga
     4561 tgccggttgt cgcagcggtc gagatcaggt gcagattctg gaacggacgc aggtcgagca
     4621 ggtcgaacac cgaaccgagg tcgacgtgaa atgcgtcggc gcgctggccg gcgaagaccg
     4681 tgcggttacc agcgagaccg tgcacgcact gcgcggccaa gtgctcgtag ttcggggtag
     4741 agcgcgggcc gatgttgcac ggcggtactt cgaggttcga gccgagcacc ttcgtgctgc
     4801 cgttacgggt cgtgcgggtc accgtgtagt actggcgacg gttgaagtgc gagtcgaaga
     4861 tcgacgtgat ctggccggtg ttgtagagga acgtgttctt gttgaacacg accgtgtgga
     4921 agcggaagcc gtaggtgatg tcggcctgtg cgtcgccgcg gttcgagatg tgaatctcgt
     4981 agagaacgtc gtcgccgaac tcgaagaagt tcgggccgcc gtctggcgcc tgcaggggaa
     5041 cgtagttggc gat
//
LOCUS       DASWVF010000769         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_505958, whole genome shotgun sequence.
ACCESSION   DASWVF010000769 DASWVF010000000
VERSION     DASWVF010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505958"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..1768
                     /gene="sucC"
                     /locus_tag="VGD55_13640"
     CDS             593..1768
                     /gene="sucC"
                     /locus_tag="VGD55_13640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY0871438.1"
                     /translation="MDLFEYQARDLFAAHGVPVLRGEVATTPEQAAAIATSIGGRVVI
                     KAQVKTGGRGKAGGVKLAANADEAREHASAILGMDIKGHVVHRVLVAEASEIEQEYYF
                     SFLLDRSNRTFLAMASREGGVEIEQTAVEKPEALARVPIDAIVGVDTAKAREIAIAGG
                     YPAQILDQAADVIVALWDVFTKEDATLVEVNPLVKTPAGIVLALDGKVTLDENAAFRQ
                     PGHAALADAEAADPIEARAKAAHLNYVKLEGEVGIIGNGAGLVMSTLDVVSYAGEAFG
                     GVRPANFLDIGGGASADVMAASLGIVLSDPSVRSVFVNVFGGITACDAVANGIVAAFG
                     KLSDAGEKITAPLVVRLDGNRALEGRAILAAAGLPGVEQNDTMDGAARRVAELAAAK"
BASE COUNT          324 a          598 c          603 g          341 t
ORIGIN      
        1 ctgtaaaagc gtgaggcccc tctatttacg agatagaggg gcctcactta ggtcggcgtt
       61 gccgccgacc tgtctccgac ccgtgtcggt agctgcggct cgaccgccgt cgatcgcgct
      121 gatcaggagt tgcctccacg acctgcacgg accgaggttt ctgaaccacg atccggccac
      181 ctcttcggac tgtgctgaac ccaggttgcc ctgcgctctg catcgtcttc ggagagccga
      241 acccgaaagt tcatttcgac cgcctcgacg cgggttaccc ctcgtcgcgc tttgtcgatc
      301 tgatgcgccg gttcgcttgc gttttggtcg acctcggtta cccgcggttt gcctctactt
      361 cgcttgccgg aaccttccgg agccgccgcc gtaatcggtg acgtgggaga tttctagcgg
      421 tcctacaagc gggccgcaag gccttggaca caacaacttt tgagtttttt tgtggtccac
      481 agaacgcaac gagttgtccc ctgaattccc gtagttctcc accggttgtg caccggttca
      541 tccacagcca ccccagtcga ccgccgtccg cgatgctcga tagcctcatc gcgtggacct
      601 gttcgagtac caggcacgag acctgttcgc cgcacatggc gtgcccgtgc ttcggggcga
      661 agtggccacg actcctgagc aagccgccgc gatcgccacg tccatcggcg gtcgtgtcgt
      721 catcaaggca caggtgaaga ccggcggtcg tggcaaggcg ggcggagtga agctcgccgc
      781 caacgccgac gaagcgcgcg aacatgcgag cgcgatcctc ggcatggaca tcaagggcca
      841 cgtggtgcac cgcgtcctgg tcgccgaggc aagcgagatc gagcaggagt actacttctc
      901 gttcctgctc gaccgatcga accgcacgtt ccttgcgatg gcatcgcgcg aaggcggcgt
      961 cgagatcgag cagaccgcgg tcgagaagcc cgaagcgctc gcccgtgtgc cgatcgacgc
     1021 gatcgtcggc gtcgacaccg caaaagcgcg ggagatcgcg attgccggcg gctacccggc
     1081 acagatcctc gaccaggctg ctgacgtgat cgtcgcgctg tgggatgtct tcacgaaaga
     1141 agacgcgacc ctcgtcgagg tgaacccgct cgtcaagacg ccggccggca tcgtgcttgc
     1201 actcgacggc aaggtcaccc tcgacgagaa cgccgcgttt cgtcagccag ggcacgccgc
     1261 tcttgctgac gccgaggccg ctgatccgat cgaagcgcgc gcgaaggcgg cccacctgaa
     1321 ctacgtgaag ctcgagggcg aagtcggcat catcggcaac ggcgcgggcc tggtgatgag
     1381 caccctcgac gtcgtctcgt atgccggcga agcgttcggc ggagtgcggc cggcgaactt
     1441 cctcgacatc ggtggcggtg cgtcggctga cgtcatggcc gcaagtcttg gcatcgtgtt
     1501 gtcagacccg tcggtgcgat cggtgttcgt caacgtgttc ggtggcatca ccgcgtgcga
     1561 cgcagtggcc aacggcatcg tggctgcctt cgggaagttg agcgatgccg gcgagaagat
     1621 cacggcaccc ctcgtcgtcc ggcttgatgg aaatagggca ctggagggtc gcgcgatact
     1681 tgccgccgcg gggctgccag gggtcgaaca gaacgacacg atggacggcg cggcgcgacg
     1741 cgtcgccgaa ctcgccgcgg cgaaatagag ggggggactg tggcgatctt tctcgaccgc
     1801 acgagtcgcg tcatcgtgca gggcatcact ggttctgagg gcagcaagca cacccgccgc
     1861 atgctc
//
LOCUS       DASWVF010000770         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_149079, whole genome shotgun sequence.
ACCESSION   DASWVF010000770 DASWVF010000000
VERSION     DASWVF010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149079"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VGD55_13645"
     CDS             <1..1012
                     /locus_tag="VGD55_13645"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0871439.1"
                     /translation="GAGQIPARQAAVAAGIPMTVPALTVNKVCLSGLDAIALADQLIR
                     AGEFETVVAGGMESMTNAPHLLPKSRAGFKFGTVDMLDSMAYDGLTDPFEHIAMGEST
                     ERYNARLNITREEQDEFAARSHQRAALARKDGLFDDEIVAVPVAQRRGDPIMVTQDEG
                     IREDTTTQSLARLRPAFSPDGTITAGSASQISDGACAVLVTSKGNAERRGWPILAEIG
                     AHGVVAGPDTSLHSQPSHAIARALAREQLAPAEIDLFEINEAFAAVAIQSMRELDIDA
                     RVVNVNGGAIALGHPIGMSGARLALHLIYELRRRGGGLGVAGLCGGGGQGDALVLRVA
                     AP"
     gene            1023..>1855
                     /gene="meaB"
                     /locus_tag="VGD55_13650"
     CDS             1023..>1855
                     /gene="meaB"
                     /locus_tag="VGD55_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY0871440.1"
                     /translation="MTRDVEGLVQAARSRDARALGKLISMVEDDDPALPALMSALLRP
                     GVQASYVIGLTGAPGVGKSTTASALISLWREREQRVGVLAVDPTSPFTGGALLGDRVR
                     MQTHATDNDVYIRSMASRGKLGGMAASTPHAVRVLEATGFDIVLVETVGVGQVEVDVA
                     ATADTTVVLLAPGMGDGIQAAKAGLLEVADLLVVNKCDRDGADAAVRELHNMVAAGGE
                     RGPGEWRPVVLRATATAAGGIDELVEAIDKHRGWLDSSGEGGRRRIRRAVQEIRSIAV
                     AA"
BASE COUNT          306 a          594 c          660 g          295 t
ORIGIN      
        1 gggagccggc cagattccgg cccgacaggc tgctgtggcg gccggcatcc cgatgacggt
       61 tccggcactg acggtcaaca aggtctgctt gtccgggctc gacgcgatcg cgctggccga
      121 ccagctcatc agggccgggg agttcgaaac cgtcgtcgcg ggcggcatgg aatcgatgac
      181 gaacgcaccg cacctgctgc cgaagtcgcg cgccgggttc aagttcggca ccgtcgacat
      241 gctcgactcg atggcctacg acggtctgac cgacccgttc gagcacatcg cgatgggcga
      301 gtcgaccgag cgatacaacg cgcgcctcaa catcactcgc gaggaacaag acgagttcgc
      361 agcccgctca catcagcgcg cagcgctggc ccgcaaagac ggattgttcg atgacgagat
      421 tgtcgcggta cccgtggcgc agcgtcgcgg cgatccgatc atggtgaccc aagacgaggg
      481 catccgcgaa gacaccacca ctcagtcgct cgcgagattg cgtccggcgt tctccccgga
      541 cggcaccatc actgcaggca gcgcatcgca gatctccgac ggtgcgtgcg ccgtgctggt
      601 cacgtcgaag ggaaatgccg aacgccgagg gtggccgatc cttgccgaga tcggtgcgca
      661 cggggtggtc gcaggccccg acacttcgct gcactcgcag ccgtcgcacg cgatcgcccg
      721 tgccctcgcc cgcgagcagt tggcacccgc cgaaatcgat ctgttcgaga tcaacgaggc
      781 attcgccgcg gtcgcgatcc agtcgatgcg cgaactcgac atcgacgcac gcgtcgtcaa
      841 cgtcaacggc ggggcgatcg cacttgggca cccaatcggg atgtccgggg cgcgcctggc
      901 cctgcatctg atctacgagc tccgccggcg cggcggtggc ctgggtgtgg cgggtctttg
      961 tggtggcggc ggccagggtg acgcattggt tctgcgcgtc gcagcgccct agaacgagtg
     1021 caatgacgcg ggacgtcgag gggctggtgc aggcggctcg gagccgcgat gcccgtgcac
     1081 ttggaaagct gatctcgatg gtggaggacg acgaccccgc actgccggca ctgatgtcgg
     1141 cacttctgcg gcccggcgtt caggcgtcct acgtcatcgg gctcactggc gcgccgggcg
     1201 tggggaagtc caccacggcg tccgcgctga tctcgctctg gcgtgaacgc gagcaacgag
     1261 tcggtgtgct cgcggtcgat ccgacgtcgc cgttcacggg tggtgcgtta cttggcgacc
     1321 gcgttcggat gcagacgcac gccaccgaca acgacgtcta catcaggtcg atggcgtcgc
     1381 gcgggaaact cggcggcatg gcggcgtcga caccgcatgc agtgcgggtg ctcgaggcga
     1441 ctggcttcga catcgtgctt gtcgaaaccg ttggggtcgg acaggtcgag gtcgacgtcg
     1501 cggcgacggc cgacacgaca gtcgtgctcc tcgcgcctgg catgggcgac ggcattcagg
     1561 cggcgaaggc cgggctgctc gaggtcgccg atctgctcgt tgtcaacaag tgcgacagag
     1621 acggggccga cgccgcggtg cgcgagttgc acaacatggt cgctgccggt ggcgagcgcg
     1681 ggccagggga gtggcgaccc gtcgtgctgc gcgcgaccgc aaccgccgct ggcgggatcg
     1741 atgagttggt cgaggccatc gacaagcacc gagggtggct cgattcatcg ggtgagggcg
     1801 gccgccgtcg gatccgacga gccgttcagg aaatcaggtc gatcgccgtt gcggc
//
LOCUS       DASWVF010000771         6333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_429524, whole genome shotgun sequence.
ACCESSION   DASWVF010000771 DASWVF010000000
VERSION     DASWVF010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429524"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4455
                     /locus_tag="VGD55_13655"
     CDS             <1..4455
                     /locus_tag="VGD55_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY0871441.1"
                     /translation="NAGATRDDVVIEAPAATPVREILTHLRALVGARADAPASIGGVS
                     IDGALAQRPLGETALRNGAIVLLEPESCVSSQPTSGHPRLLDAPMPSHLELRVVGGPV
                     AGTVYPVAMGTTSLGRFRGPGISLDDPAVSRHHAMLTVSADAVTISDVGSTNGTMLDG
                     VPVGSRPVPVRPGMRLRLGESTLLVTAPDEPFVAITSTTDGRLAFNRPPRLDAPDPDA
                     AHRAVAFPTEPVDRSPNRLPLVATLAPLIAGVALAAVMRRPEYLLFTVLSPLMMASQW
                     VADRLGHRKSKRAEQVAYEVALAKAESASVDALSADARERRSRAPDPVALLSAVGADR
                     QHPHTPRSQGAPSARLWERRSADADFLVVSVGLGTDRASVDVSGTRFGIPMPPPSVSD
                     VPVTVSLPAIGILGIAGAPHATASLARAIVGQIAVLHSPRDVGLVVLAEPSRANTWEW
                     VRWLPHLQPVSGANCQTLLGLDAQSAASRVAELLALIEYRSDNSMTSASPRAIVVLVE
                     GAQALRRTPALAQLLAHGPSVGVYAICVDSDERRLPEECGAVVRFDDPVSSRLHLTVS
                     GAPPIIDVVGDGVSITWAENLARALSPICDDSPDRSGGLPDSVRWLDIAGIADITADL
                     PARWRAPGTGTTQALIGAGPNGRFVIDIARDGPHALIAGTTGSGKSELLQTFVASLAL
                     ANRPDEMTFVLVDYKGGAAFGACATLPHTVGMVTDLDGRLVERALTSLGAELKRREGV
                     LAAAGAVDIARFRADGGVLARLVIVVDEFASLAEELPSFVGGLVAIAQRGRSLGVHLV
                     LATQRPEGVVSADIRANANLRICLAVMRDAESRDVIDTPNAARISRTTPGRGYARTGH
                     GELVAFQTGRVGGTSARDDGEIDVRLSPFRALCQPAARQSRARGVNESAETDLDCIVT
                     ACRDAACHLGITSAPSPWLPPLPEVIPLTAGLTADDRRPLTAVLGVLDVPSLQARQNY
                     VVDLAVLGHLVIAGSARSGRTTALRTLAGGLAKSTPSDQLHLYAVDCSGGSLGALTVL
                     PQCGAVVSNHEPARVLRLFSTLTAELHRRQAEIAPLAQAGPTRPHIVLLIDGWEAFLA
                     AFEDLDAGAIVDGCTRLLREGAAADIHVIATADRAGLVGRLASTVENRLVLRLADRAD
                     FSLIGLPARSVPLALPPGRGFVVDGLVEMQICALGRDPSGPAQVAELDAIAHECRTRD
                     TTVASARRPRRIDPLPTVIARTEIESESRRPLPPPGSPRVTLGVGGDELTAVYVDLLD
                     TGPGFLVAGPHRSGRTTALATIADGARASGWHVVAVTPRSSALRDHADDVFDAGGVGL
                     AEALARVDGRLAVLVDDAELIADSPVASDLDRLMRVARDGGHIVVIAGSTDEMSIGFR
                     GFLVDARRARSGILLHPRGPLDGEVLGVRLPRNSGGAAPPGRGLLVVRGSITHMQVAM
                     PDPRPDPRPDPPRRRRQTQPQTVTDRVPIAAPPCATVTLTTPSWAPLK"
     gene            complement(4362..4919)
                     /locus_tag="VGD55_13660"
     CDS             complement(4362..4919)
                     /locus_tag="VGD55_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871442.1"
                     /translation="MTFKDTELNRASLGGLKSLRSAAAVVVAFSGSAALLAGCTSSHP
                     SATAQSSAPASSAPASSPAPALSSVPITAFPASPLASAGSSLASPVSSEVPGASLIAN
                     IEAPATKGGFQIRLHRGSILQVTVPQAKAGTPVTFALVPATSTFLTPIAGHDGYFSGA
                     QDGVVNVTVAQGGAAIGTLSVTVWG"
     gene            5069..5668
                     /locus_tag="VGD55_13665"
     CDS             5069..5668
                     /locus_tag="VGD55_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871443.1"
                     /translation="MSVADTLEQGGEPHPALPAVRRAAYTLGVIGVVVFVTVQVLHSI
                     DRREQRASFDRMLSLSAGAQAAVEQAVSQERDTAQYAEPLLNSSLTTQAVREHLYVAV
                     SASALQAQRDIDAQVQRIQADSTGRSGRLRSARDAMLAYLASWSALFASAAGSSNAPA
                     DSQTDLDAQQVAARTALEKAAPDPVRAAKARVVFGTTTA"
     gene            complement(5779..>6333)
                     /locus_tag="VGD55_13670"
     CDS             complement(5779..>6333)
                     /locus_tag="VGD55_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0871444.1"
                     /translation="AVRSLRGRAARQAFRQVAGRCRSGSLINVEPIGWVRSSRAGAID
                     DDWDAVTATIGLDAAQFTPDAVAGLEAFSHIEVVFVFDQVEIADVESGARHPRGNVEW
                     PRVGIFAQRAKMRPNRVGVTVCRLLEVDGLVLRMAGLDAIDGTPVLDIKPYMREFGAR
                     GEVRQPQWSHELMAGYWTNPDRRI"
BASE COUNT         1047 a         2167 c         2068 g         1051 t
ORIGIN      
        1 aacgcggggg cgactcgcga cgacgtcgtc atcgaggctc cggcggcgac accggtccgc
       61 gaaatcctca cccaccttcg ggcactggtg ggtgcccgtg cggacgcgcc ggcatccatt
      121 ggcggcgtca gcatcgacgg cgcgctcgca cagcgaccgc tgggtgaaac ggcactccgc
      181 aacggcgcga tcgtgctgct cgaaccagag tcctgtgttt ccagccagcc gacgagcggt
      241 cacccgcgct tgctcgatgc gccgatgccg agccacctcg aactccgggt cgtcggtggg
      301 ccggtggcag gcaccgtcta cccggtcgcg atgggaacga cgtcgctcgg cagattccga
      361 ggaccaggaa tttcgctcga cgaccccgcc gtgtcgcggc atcacgcgat gctcactgtc
      421 agcgccgacg ccgtgaccat ttccgacgtt gggtcgacga acggcacgat gctcgacggg
      481 gtgccggttg gtagtcgccc ggtaccggtg cgacccggca tgcgcctgcg cctcggcgag
      541 tcgacgctgc tcgtcactgc gcctgacgaa ccctttgtcg cgatcacgtc gacaaccgac
      601 gggcggctgg ctttcaatag gccaccacgc ctcgatgcac ctgatcctga tgccgcgcac
      661 cgggcggtgg cgttcccgac cgagccagtg gacagatcac cgaacaggct gcccttagta
      721 gcaacgcttg cccccttgat cgccggcgtc gcgctggccg ccgtcatgag gcgtcccgag
      781 tacctcctgt tcacggtgtt gtcgccgctg atgatggcct cgcagtgggt ggccgatcgc
      841 ctcgggcatc gcaagagcaa acgcgccgaa caggtggcct atgaagttgc gctcgctaag
      901 gcggaaagcg cgagcgtcga cgcgttgtct gctgatgcgc gggagcggcg ctcccgagca
      961 cctgacccgg tggcgttgtt gagcgcagtc ggtgcggacc ggcagcaccc ccacacgccc
     1021 cgctcccaag gcgctccaag cgcccggctt tgggaacgcc gatccgcaga cgctgacttt
     1081 ctcgtcgtca gtgtggggct cggaacagat cgcgccagcg tcgacgtcag cggcacccgg
     1141 ttcggcatac ccatgcctcc gccatccgta agcgacgtcc ccgtgaccgt gtcgctgccc
     1201 gcgatcggaa tcctcgggat cgccggtgcg ccgcacgcga ccgcatccct agcgcgcgcg
     1261 atcgtcggtc agatcgcagt gctccacagc ccgcgtgatg tcgggctggt ggtcctggcc
     1321 gaaccttcgc gagcaaatac gtgggagtgg gttcgctggc tgccacacct gcagcctgtg
     1381 tcaggggcca actgccagac gcttcttggt cttgacgccc aaagcgcggc ctcacgggta
     1441 gccgaactgt tggcgctcat cgagtaccgc agcgacaaca gcatgaccag cgcctcaccg
     1501 cgcgccatcg tggtgctcgt cgagggcgca caggcgttgc gccgtacgcc ggcgctagcg
     1561 cagctactcg cacatggccc atcggtcggt gtgtatgcga tttgcgtcga cagcgatgaa
     1621 cgacgtctgc ctgaagaatg cggggcggtg gtccggtttg acgacccagt gagcagccga
     1681 ctgcacctca cggtgtcggg agctccgccg atcatcgacg tagtcggcga cggtgtctcg
     1741 atcacgtggg ccgaaaacct tgcccgcgcc ctgtccccca tctgcgacga cagccccgat
     1801 cgttccggcg ggctacctga cagcgtccgt tggctcgaca tcgccggcat tgccgatatc
     1861 acagccgatc taccggcgcg ttggcgcgcg ccagggaccg ggaccacaca ggcactcatc
     1921 ggtgccggcc caaatggcag gttcgtcatc gatattgcgc gcgacggccc ccacgcgctc
     1981 atcgccggaa ccacaggttc gggcaagagc gagttgctgc aaaccttcgt cgcgtcgctc
     2041 gccctggcta accgtcccga cgagatgacg ttcgtgctgg tcgactacaa gggtggcgcg
     2101 gcgttcggag cgtgcgcgac gctaccgcac accgtcggca tggtcaccga tctcgacggt
     2161 cgcctggtcg aacgggcgct gacctccctc ggcgcggagt tgaagcgccg cgaaggagta
     2221 ttggctgcgg caggggcggt tgacattgca agattcagag cggacggcgg tgtgctggcg
     2281 cgtcttgtca tcgtggtcga tgaattcgca tctctggcag aagaattgcc gtctttcgtc
     2341 ggcggccttg tcgccatcgc ccaacgcggc cgctcgctag gcgtccatct cgtacttgcg
     2401 acccaacgcc ccgaaggtgt cgtgagcgcc gacatccggg ccaacgcgaa cttgcgaatc
     2461 tgtctagcgg tgatgcgcga cgctgaatcg cgcgatgtga tcgatacgcc caacgccgct
     2521 cgtatcagtc ggaccacgcc tggccgcgga tacgcccgca ccgggcacgg cgaactcgtc
     2581 gcgttccaga cgggtcgcgt cggaggtacg tccgctagag acgacggcga gatcgacgtc
     2641 aggctgtccc cattccgtgc gctgtgccaa ccggccgcac ggcagagtcg ggcacgcggt
     2701 gtgaacgaat ccgccgagac cgacctcgac tgcatcgtga ccgcttgccg cgacgccgca
     2761 tgtcatctcg gtatcaccag cgcaccttcg ccgtggctgc caccgctgcc cgaggtgatc
     2821 cccttgaccg cgggactcac cgcggatgac cgccgcccgc tgaccgcggt gcttggcgtg
     2881 ctcgacgtcc cgtctttgca agcaaggcaa aactatgtgg tcgatctcgc cgttctcggc
     2941 cacctagtga tcgccggaag tgcacgaagc ggccggacca cggcgctgcg cactctggcc
     3001 ggtggccttg cgaaatcgac gccgagcgat cagttgcact tgtacgccgt cgactgctcg
     3061 ggtgggtcgt tgggtgcgct gaccgtgcta ccgcaatgtg gtgcggtggt gtcaaaccac
     3121 gaacctgcac gggtcttgcg gctgttttcg acactcacgg cggagctcca ccgacgtcaa
     3181 gcggagatcg cgccgctcgc acaagccgga ccaacgagac cacatatcgt tttgctgatc
     3241 gatgggtggg aggcattcct agcggcattc gaggatctcg acgcgggcgc aatcgtcgat
     3301 gggtgcacac gcctcttgcg tgagggtgcg gctgcggaca tacatgtcat cgcgaccgcg
     3361 gatcgagctg ggctcgtcgg tcggctcgcc tcgaccgtcg agaatcgctt agtacttcga
     3421 ctggccgacc gcgccgattt ctcgctcatc ggactgccag ccaggtcggt gccgctcgca
     3481 ctgccccccg ggcgcggctt cgttgtcgac ggcctcgtcg agatgcagat ctgcgccctc
     3541 ggtcgagatc cgtcaggccc tgctcaagtc gccgaactcg acgcgatcgc gcatgaatgt
     3601 cgcacgcgtg acacgaccgt ggcatcggct cggcgaccgc gtcgcatcga ccctctcccg
     3661 acggtgatcg cgcgtaccga gatcgagagc gaatcgcgaa ggcccctccc gccaccgggt
     3721 tcgccgcgcg tcacactcgg tgtcggtggg gatgagctga cggcggtcta tgtcgatctc
     3781 ctcgacaccg gtccgggctt tctcgtcgcg ggcccgcacc ggtccggtcg gactaccgcg
     3841 cttgcgacta tcgctgacgg cgcccgagca agcggctggc acgtggtcgc cgtcacgccg
     3901 cgctcgtcag cgcttcgcga ccacgccgac gacgtcttcg acgctggcgg tgtcggattg
     3961 gcagaagcgc tggcccgggt cgacggtcgc ctcgcggtct tggtcgacga cgcagaactc
     4021 atcgccgact cacctgtcgc gagcgatctc gatcgactca tgcgagtcgc tcgcgatggc
     4081 gggcacatcg tcgtgatcgc cgggagcacc gacgagatga gcatcggttt tcgtggcttt
     4141 ctggtcgacg caaggcgcgc tcgtagcggc attttgttgc acccgcgcgg cccgctcgac
     4201 ggcgaggtgc tcggcgtgcg actgccgcgc aacagtggtg gcgctgcgcc cccgggtcgt
     4261 ggcctgctgg tggtccgggg gtccatcaca cacatgcagg tggcaatgcc agaccccagg
     4321 cccgacccaa ggcccgaccc acctcgtcgg cgtcggcaga ctcagcccca gaccgtgacc
     4381 gacagggtgc cgatcgcggc gccaccctgc gcgaccgtca cattgacgac cccatcctgg
     4441 gcgccgctga agtagccgtc gtgccccgcg atgggcgtca gaaaggtcga ggtcgccgga
     4501 acgagtgcga aggtcacagg ggtgcccgcc ttggcttgcg ggacggtgac ctgaaggatc
     4561 gagccgcggt gcagcctgat ctggaacccg cccttggtcg ccggcgcctc gatattggcg
     4621 atcagcgatg cgccgggaac ctcactcgac accggacttg ccaggctcga acccgcagag
     4681 gcgagcgggc tcgccgggaa ggcggtgatc gggacgctcg ataacgccgg agccggagac
     4741 gacgcgggcg cgctcgacgc gggcgccgac gactgtgccg tggcgctggg gtggcttgag
     4801 gtacatccag ctagcagggc agccgagccc gagaaagcta cgaccactgc ggcagccgat
     4861 cggagagact tcaaaccgcc gagtgaggcg cggttgagtt cggtgtcctt gaaggtcatc
     4921 gctccaccct aacccgagcc acctatggca cccgctcccg gcacgccgcg atgagcgatc
     4981 gcgggcgccg ggcgatcaca tccaatcgag ccgcacgcaa cgaacgcagc gttgtgtcgg
     5041 cgtacccgct cagcgcatgc tctaactcat gagcgtcgcc gacacgttgg agcaaggtgg
     5101 cgagccgcac cctgcacttc ctgccgttcg gcgcgccgca tacacgctcg gcgtgatcgg
     5161 cgtcgtcgtt ttcgtcacag tccaggtctt gcactcgata gatcgacggg agcagcgtgc
     5221 gtcgttcgac cgaatgctga gcctgtcggc aggtgcccag gccgcggtgg agcaagcggt
     5281 atctcaggag cgcgacacgg cgcagtacgc cgaaccgctg ctgaactcct ccctcacgac
     5341 acaagcggta cgcgagcacc tgtacgtcgc cgtgagcgcg agtgccctac aagctcaacg
     5401 cgatatcgat gcacaggtac agcgcatcca ggccgactca accgggcggt caggtcgact
     5461 ccgcagcgcg cgcgatgcaa tgctcgcgta cctagccagc tggtcggcgc tcttcgcaag
     5521 cgccgccggc tcaagcaacg cacccgccga ctctcaaacc gacctcgacg cgcaacaggt
     5581 cgccgcccgt accgccctcg agaaggctgc gccggacccg gtgcgggccg ccaaggcacg
     5641 cgtggtcttc ggcacaacga cggcgtaaca acaatcggga gcgcgtcact tgccgtcgac
     5701 gttggcggcc cgcctcgagt cgagcgaggg ctagctcgag ttcgggaagt tggagtccgt
     5761 gcatgacgga ccggggggtc agatgcggcg gtccggattg gtccagtagc cggccataag
     5821 ttcgtgcgac cactgaggtt ggcggacctc acctcgggcc ccgaactcgc gcatatacgg
     5881 ctttatgtcg agcacgggtg tgccatcgat cgcgtcaagc ccggccatcc gcagcactag
     5941 gccgtcgacc tcgagcagcc gacacacggt gaccccaacc cggttcggcc gcatcttcgc
     6001 gcgctgggcg aagatgccaa cccgaggcca ctcgacattg ccccgcggat gtcgcgcacc
     6061 actctcgaca tcagcaatct cgacctggtc gaacacgaac acgacctcga tgtgggagaa
     6121 cgcctcgagt ccagccactg catcaggggt gaactgcgcg gcatccaagc cgatcgttgc
     6181 cgtcaccgca tcccagtcgt cgtcgatcgc tccggcgcgc gacgatcgca cccacccgat
     6241 cggttcgacg tttatgaggg agccgcttcg gcatcgacca gcgacctggc gaaatgcctg
     6301 gcgagcggcg cgcccacgta aggaccgaac ggc
//
LOCUS       DASWVF010000772         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_225956, whole genome shotgun sequence.
ACCESSION   DASWVF010000772 DASWVF010000000
VERSION     DASWVF010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225956"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VGD55_13675"
     CDS             complement(<1..2183)
                     /locus_tag="VGD55_13675"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEY0871445.1"
                     /translation="MSFTSAHPFAGFGANEWLVDEMYQQYLKDPASVDKAWWDFFADY
                     SPSDAPAEPAPAPAANPAPAPASAVPAQTTPVPPVTASTPAVAADASESASNGVVVST
                     GSTSSAVQDEVLPLKGAAARTVANMEASLAVPTATSVRDIPAKLVADNRLVMNNHLKR
                     GRGGKISFTHIIGYAVVRAAVALPEMNAAYTEINGKPGVVHHHDVNLGLAIDMTKDDG
                     SRQLLVPSIKGSQNLDFAQFWMAYEDVVRRARSNKLTADDFAGTTITLTNPGTLGTVH
                     SVPRLMQGQGCIVGVGAMEYAAQFQGASAETLANLAVSKVMTVTSTYDHRVIQGAQSG
                     EFLRQIHRLLLGEDGFYDDLFRALRIPYEPVRWVQDIVSTHDDEVGKPARVIELIHAY
                     RVRGHLMADTNPLEFEMRKHPDLDIVNHNLTLWDLDREFATGGFGGVPVMKLRDILGV
                     LRDSYCRTIGIEYMHISDPVQRRWIQDRIEQAHSKASREEQLYILNRLNVAEAFETFL
                     QTKYVGQKRFSLEGGETLIPLLDSILDESARKGLDEVVIGMAHRGRLNVLANIVGKSY
                     GQIFREFDGNMDPNSAHGSGDVKYHLGTNGRYTSQHGASINVSLASNPSHLEAVDPVL
                     EGIVRAKQDALNKGAAYPVLPVAIHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVVV
                     NNQVGFTTSPLSARSSVYATDVARMIQAPIFHVNGDDPEAAVRVAR"
     gene            2369..3559
                     /locus_tag="VGD55_13680"
     CDS             2369..3559
                     /locus_tag="VGD55_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0871446.1"
                     /translation="MRRTAAVVTSLALAAIFAVGSSTPAFAADRTAANPPTPQTLAAL
                     RAQADAVRAELTAGAAKLDAARSKLASLQATAAAATAEASKIDAGLGAMRDQLAGYAA
                     QLYMEPTTETAMSALTEGPNFAASVQGVQMLSIVNRGRTEVLRAVVVDEEQAQVLQTQ
                     AAQAVAAAAIVQKSVTSQVAALQAKAGQASTRLTAGEVAYQSALERAAVQRIAAAKAA
                     HNETARALAVRQAAALAADEADIPVPAAQCGAASDGPFPAGPWGGYADGFIPASQLCA
                     ITGGGRLRPDAAAAFNAMSAAYARTFGSGLCVSDSYRSYSQQVRVFRQRPSLAAVPGT
                     SNHGWGLAVDLGCGVQSSRSAQYRWMSANAGTYGWVHPAWAFHDPFEPWHWEFGHLAG
                     AGGT"
BASE COUNT          629 a         1256 c         1339 g          703 t
ORIGIN      
        1 cgcgcgacgc gtacggccgc ctcgggatcg tcgccgttca cgtgaaagat cggcgcctgg
       61 atcatccggg cgacgtcggt ggcgtacaca ctcgagcgcg ccgacagcgg cgacgtcgtg
      121 aagcccactt gattgttgac cacgacgtgc acagtgccgc ccgtgcgata cccgcgcagc
      181 tgtgacaggt tcagggtctc ggcgaccaca ccctggcccg cgaacgccgc gtcgccgtgg
      241 atcgcgactg gcagcaccgg gtaagcagcg cctttgttca gcgcgtcctg cttcgcccgc
      301 acgatgcctt cgagcacggg gtcgactgct tcgaggtggc tggggttcga ggcgagtgag
      361 acgttgatcg atgcgccgtg ctgcgacgtg tagcggccat tggtgccgag gtgatacttc
      421 acgtcgcccg agccgtgcgc ggagttcggg tccatgttgc cgtcgaactc acggaagatc
      481 tggccgtagc tctttccgac gatgttcgcg agcacgttga ggcggccgcg gtgagccatg
      541 ccgatgacaa cctcgtcgag gcccttgcgc gccgactcgt cgagaatgct gtcgagcaac
      601 ggaatgagcg tctcaccgcc ttcgagcgag aaccgcttct gcccgacgta cttcgtctgc
      661 aggaacgtct cgaaggcctc ggcaacgttg agcctgttca ggatgtacag ctgctcctcg
      721 cgactggcct tgctgtgcgc ttgctcgatg cggtcttgga tccagcgccg ctggacgggg
      781 tcggagatgt gcatgtactc gatgccgatc gtgcggcagt acgagtcgcg cagcacaccg
      841 agaatgtcgc ggagcttcat cacagggacg ccgccgaacc cgccggtcgc gaactcgcga
      901 tcgaggtccc acaacgtgag gttgtggttc acaatgtcga ggtcggggtg cttgcgcatc
      961 tcaaactcga gcggattcgt atcggccatg agatgcccgc gcacccggta ggcatgaatc
     1021 agctcgataa cgcgagccgg cttgccgacc tcgtcgtcgt gggtcgacac gatgtcttgc
     1081 acccagcgca ccggctcgta cgggatgcgc aacgcgcgga acaggtcgtc gtagaagccg
     1141 tcttcgccga gcagcagccg gtggatctgc cggagaaact cacccgactg cgcaccctgg
     1201 atcacccggt ggtcataggt cgacgtgacc gtcatgacct tgctgacagc gagattggcg
     1261 agcgtctcgg ccgacgcgcc ttggaattgc gcggcgtatt ccatcgcgcc tacaccgacg
     1321 atgcagcctt gcccctgcat cagcctcggc accgaatgca ccgtgccgag cgtgccggga
     1381 ttcgtgagcg tgatcgtggt ccccgcaaag tcgtcggccg tcagcttgtt cgaccgcgcg
     1441 cgccgaacga cgtcctcgta tgccatccag aactgggcga agtcgagatt ttggctgccc
     1501 ttgatcgacg gcacgagcag ttgcctgctg ccgtcgtcct tggtcatgtc gatcgcgagc
     1561 ccgaggttga cgtcgtggtg atgcacgacg cctggcttac cgttgatctc ggtgtacgcc
     1621 gcattcatct caggcagtgc aacggccgct ctgacgactg cgtaaccgat gatgtgggtg
     1681 aacgagatct tcccgccgcg cccgcgcttg aggtggttgt tcatcaccag gcggttgtcg
     1741 gcgaccagct tggccgggat gtcacgcacg ctggtcgcag tcggtaccgc gaggctcgct
     1801 tccatgttcg cgacggtgcg tgcggctgcg cccttgagcg gcagcacctc gtcctgaaca
     1861 gcggagctgg tggagccggt agagacaacg acgccgttgg acgccgattc gctggcgtca
     1921 gctgcaacgg ccggcgtact cgcggtgaca ggagggacag gtgtggtctg cgcaggcact
     1981 gccgaggctg gcgccggagc ggggttggcg gccggtgcgg gtgcgggctc ggcgggtgcg
     2041 tcgctcgggg agtagtcggc gaagaagtcc caccaagcct tgtcgaccga ggccggatcc
     2101 ttcaggtact gctggtacat ctcgtcgacg agccattcgt ttgcgccaaa tccggcaaac
     2161 gggtgggctg atgtgaaaga cactgcgggt ttcgcctctt tgcaggagcg gaaggtttag
     2221 gcggctaggg cgtcagactc ccaggctacc cgtagacgat aagtcgccgg aggtagcatc
     2281 gccgccaacc acgacgtcct cgagccctag ggactcctca gatcccgtga ctgattgccg
     2341 gcccgttcgc agcccggttg gctgcggcgt gcgccgcact gcggcagttg tgaccagcct
     2401 cgccctcgcg gcgatcttcg ccgtcggctc aagcacgccg gccttcgccg ccgaccgaac
     2461 agcagccaat ccgccgaccc cacagaccct cgctgccttg cgggcgcaag ccgacgccgt
     2521 ccgcgctgaa ctgactgctg gagcggcgaa gctcgacgcg gctcgatcga agctcgccag
     2581 cctccaagcg accgctgcgg ccgctactgc ggaggcgtcc aagatcgacg cggggcttgg
     2641 cgcgatgcgc gaccagctgg cgggctacgc agcacagctc tatatggagc cgacgaccga
     2701 gactgcgatg agcgcgctga cggaggggcc gaatttcgcc gcgtcggtgc aaggcgtgca
     2761 gatgttgtcg atcgtcaacc gcggtcgcac cgaagtgctt cgcgcggtgg tagtcgacga
     2821 ggagcaagcg caggtcttgc agacgcaggc cgcccaagct gtggctgcgg cggcgatcgt
     2881 gcagaagtcg gtcacttcgc aagtcgccgc gttgcaggca aaggcgggtc aggcgtcgac
     2941 ccggctgaca gccggcgagg tggcctacca atccgcattg gagcgggctg ccgtgcaacg
     3001 catcgcggcc gcgaaggcgg cgcacaacga gacagcccga gccctcgcgg tgcggcaggc
     3061 ggcggcgctt gcagctgacg aagctgacat tccggtgccg gcggcgcaat gtggcgcggc
     3121 atccgacggt ccgttcccgg ctggtccatg gggcggctac gccgacggtt tcattcccgc
     3181 atcgcagcta tgcgcgatca ccggcggcgg ccggctgcga cccgacgcgg ccgctgcgtt
     3241 caatgcgatg tcagcggcct acgcgcgcac gttcggttcg ggcttgtgcg tcagcgattc
     3301 gtaccgcagt tactcccagc aggtgagggt gttccggcaa cggccgtcgc tggccgccgt
     3361 cccaggcacc agcaaccacg gttgggggct cgccgtcgac ctcggctgcg gggtgcagag
     3421 ttcgcgttcc gcccagtacc gctggatgag tgccaacgca ggcacgtacg gctgggtaca
     3481 tccggcgtgg gcgttccatg acccgttcga gccctggcat tgggagttcg gtcacctcgc
     3541 aggcgccggc ggtacctagc cccgcttgtc ggcgcttcct gcgggcgcgg gagtcagtcg
     3601 agtgggattg ccgcggccca ctcggcgaat gcatccaact taggctgact cctgccggct
     3661 gagaatcgcg gccaatccgc tatccagtcg gccccgcgtt cacgtgaacg cgaggtcttg
     3721 gtgccacgtg cgcccccgcg ttcacgtgaa cgcggccgaa tctcggtgtc ggtgaaggcg
     3781 ccggttcctg gtaggcactc ccagcccaag gtgccggagt tccctcatta ccacgatcgt
     3841 gtcgacgtcg aaacgaccgt gtcagcggca gttgcggata tttggtgatg ttcgggaggc
     3901 gcagtttgga cgcgtgagtc aggcgct
//
LOCUS       DASWVF010000773         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_301904, whole genome shotgun sequence.
ACCESSION   DASWVF010000773 DASWVF010000000
VERSION     DASWVF010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301904"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1203
                     /locus_tag="VGD55_13685"
                     /pseudo
     CDS             214..1203
                     /locus_tag="VGD55_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
     gene            complement(1216..>2115)
                     /locus_tag="VGD55_13690"
     CDS             complement(1216..>2115)
                     /locus_tag="VGD55_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0871447.1"
                     /translation="VEYSAVGFGSHHWQVVDEAGRRYFVSLDDLEARRRSAEDTHDRV
                     YERLRAALLTARTLDDSGATFVVAPVRTLDGDVLRRIRGQYAAALYPFVEGRERDFGD
                     TFPEAERGQVLRLIAVLHSSPAVLWGSAHVEDFVHPQRDEIAYALDELAAPWETGPYG
                     DRARSWFEVRANRIERTLEEYDRLAEQARQRPDRMVLTHGEPHPGNLIETTDGWLLVD
                     WDTALVAPPERDLWHLRGAEPSSFGAYTSVAGHDVVPGMLEFYRVAWELDDIASLAAR
                     FHRAHDDTEDARHEWADMQHRSL"
BASE COUNT          362 a          688 c          684 g          381 t
ORIGIN      
        1 cccagatgac cgccaccggc ttgcgcgaga cgaccgcgtc gatgatgtcc gcggtcagca
       61 acggagcgat ccacaccggc gactgcttga gcgtgaacgc gacggccgcc agcgacagcc
      121 gtcgtcgctg acccgagtac aagcgcagcg cggttcgggc cggacgagcc gggtctgaag
      181 cgcttaagtt cacaacacgg aaaggtaccc cacatggccc ggtctggctt caacctgcag
      241 tggctctaca gcaagcgaga ggctccacgt ggccctgacg agaaggtcct cgacgcgatc
      301 gcggcgtggg ggttcgactt cgtccgattg ccgacagatt atcgcctgtg gagcgtcgac
      361 gacgatccat accgccccga tgaatccgtt ctcgctctcg tcgatgaagc cctcgcggcg
      421 tgccggcaac gcgagatcca cttgtcgctg aacgtgcacc gcgcgccggg ctacgtgatc
      481 accggttggg aaactgaacc gtacaacctg tgggccgatc tgcccgccta agacgcgttc
      541 gtcgccacgt gggcacgctt cgcccagcgc tacgtttccg tgccgggcgc ggcattgagc
      601 ttcgatctcg tcaacgagcc gccagcgctg gggttgcgtg gtttcacccg cgacgcgcac
      661 gaggcggtga tccgcagaac ggtggcggcg atccgcgcgg tctcacccga ccgtccgctg
      721 gtcatcgacg ggctcgacgg tggcaatctc gcgatgcccg aacttgccga tctcggactc
      781 actcagagtg tgcgcggcta ccagcccatg cgggtgtcgc actatggggc gtcttggtgg
      841 cccggtcacg ttggtatgcc cgagccggcg tacccctgcg agtacgacgg acgttggtgg
      901 gatcgcgcgg gccttcgcga gttctaccaa ccgtggcgtg acctggaggc tttgggtgtg
      961 tcggtgcatg tcggagaatt cggttgctac gagcggacgc cggacgacgt ggcgcaggcc
     1021 tggttcaccg acctgttcgg cgtgttccaa gagttcggct ggggcttcgg cctctgggaa
     1081 ttcgagggac cattcggcat catcgggcac ggtcgcccag gggcacactt cgaagcgaga
     1141 gacggcttca tggtcgaccg ccgactactc gacctgatga tgtcggcccg ttcgaaggct
     1201 tagcgacagc acccgctaca ggctgcggtg ctgcatgtcc gcccattcgt gtcgagcgtc
     1261 ttcggtgtcg tcgtgagctc ggtgaaaccg tgcggccaga gatgcgatgt cgtcgagctc
     1321 ccaggccacg cgataaaact cgagcatccc cggaaccacg tcgtggcccg cgacgctcgt
     1381 gtacgcaccg aagctgctcg gttcggcacc ccgtaggtgc cagagatcac gctccggcgg
     1441 tgcaacgagc gcggtgtccc agtcgaccag tagccatcca tcggttgtct cgatgaggtt
     1501 gccgggatgt ggttcgccat gggtcaacac catgcgatcc ggccgttggc gtgcctgctc
     1561 ggccagccgg tcatattcct cgagcgtgcg ttcaatgcgg ttggctcgga cttcaaacca
     1621 cgatctcgca cggtcgccgt agggccctgt ctcccatggc gctgccagct cgtcgagcgc
     1681 atacgcgatc tcgtcgcgtt gcggatgcac gaaatcctcg acgtgcgcgc tgccccacaa
     1741 gaccgcgggc gacgaatgaa gcaccgcgat caaccgcaaa acctggccgc gctcggcctc
     1801 ggggaaggtg tcgccgaagt cgcgctcgcg gccctccacg aatgggtaca gtgccgccgc
     1861 atactgaccc cgaatacgtc gtaggacatc accgtcgagt gtcctgacag gtgccacgac
     1921 gaacgttgcg ccgctgtcgt cgagggtacg agcggtgagc aacgccgcac gtaggcgctc
     1981 gtacacgcgg tcgtgtgtgt cctcggccga gcgtcgtcga gcctcaaggt cgtcgaggct
     2041 tacgaaatag cgtcggcccg cctcgtcaac tacttgccag tgatggctac cgaaaccaac
     2101 cgccgaatac tcaac
//
LOCUS       DASWVF010000774         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_200332, whole genome shotgun sequence.
ACCESSION   DASWVF010000774 DASWVF010000000
VERSION     DASWVF010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200332"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGD55_13695"
     CDS             <1..204
                     /locus_tag="VGD55_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871448.1"
                     /translation="DKLVSQRVAVRAKTTFPDAEVLRLSDAGHVAHLEDPATVAEALR
                     SLVGRHDLDGGMTGRRGTVMKLI"
     gene            227..1405
                     /gene="moeZ"
                     /locus_tag="VGD55_13700"
     CDS             227..1405
                     /gene="moeZ"
                     /locus_tag="VGD55_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HEY0871449.1"
                     /translation="MSLPPLVEPAESLTVEEVRRYSRHLIIPDVAMVGQKRLKNARVL
                     AIGAGGLGSPVLMYLAAAGVGTLGIIEFDTVDESNLQRQIIHGQSDIGRSKAQSARDS
                     IHEINPLVKVVLHEERLENENVFEIFGQYDLIVDGTDNFATRYLVNDACVLLGKPYVW
                     GSIYRFDGQASVFWAENGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCASIGSIQATEA
                     IKLLTGIGDPLVGRLMIYDALEMSYRTVNIRKDPECPICGKNPTITELIDYETFCGVV
                     SDEAVLAAQGSTIVPRELKKWIDDGENIFLVDVREPAEFEIVSIPGATLIPKGEFLNG
                     SALERMPQDKRIVLYCKSGMRSAEALAIVKGAGFADAVHVGSGVVGWVNQVDPSLPSY
                     "
     gene            1480..1770
                     /locus_tag="VGD55_13705"
     CDS             1480..1770
                     /locus_tag="VGD55_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY0871450.1"
                     /translation="MPPFAQRVLDLVDAIPPGRVLAYGDIAEYLAEGGPRQVGTVMST
                     WGGAVPWHRVVHADGTPAPSHDGEVLRLLRADATPMRGDRVDMTRARWDPDS"
     gene            2015..>3862
                     /locus_tag="VGD55_13710"
     CDS             2015..>3862
                     /locus_tag="VGD55_13710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY0871451.1"
                     /translation="MLDEAQRRAVEHRGGPVLVLAGPGTGKTTTIVEAVVQRVESGEI
                     TPDQALVLTFSRRAAAELRERIAARLGRTIRDPIARTFHSYAFGLLRADAARRGEATP
                     RLLSGAEQDLVVRELLRGDVEAGAAMWPQRLHPALLTRGFAQELRDFVQRAVERGITP
                     RELAQIGRRYKRVDWVAAAKFAEQYAQVSALREAASYDPAELIRAAAGLLRSEPLVLQ
                     EIRAAHEFVVVDEYQDTDPAQEELLALLVGSRDLLAVGDPDQSIYGFRGADLEGVRRF
                     PDRFKAPDGSAPTIVALDVSRRSGANLLAASRRVAQRLSGLPAHRALRPAPGIEPGSA
                     EVHVFATANAEAAYLAHRLRELHLLHDVPWARMAVLVRSGVPLPALRRALQSAGVPVA
                     AYAEEQPLVDVPIVRALLRVLEVVTGRLDFEGPQGVELAQELVTGPLGRADPLALRRL
                     RQELRSMELAAGGGRASGALLATALTTPADLVPIERRIAIPALRIAAVLQAGRAAVAE
                     PSATAEAVLWAIWQAAGRAPRLSSLALSGGPGSAAADRDLDAAVALFDAAARFADRLP
                     KAGPSVFLDHLLGQQIPGDTLAPQAPTADGVRLMTAHAAKGLEWDVVVVA"
BASE COUNT          580 a         1260 c         1345 g          677 t
ORIGIN      
        1 gacaagctcg tgtcgcagcg agtcgcggtt cgggcgaaga caactttccc ggacgccgag
       61 gtgctgcggt tatccgatgc aggtcacgtc gcccacctcg aggatcccgc gaccgtggca
      121 gaggcgctgc gctcgttggt cggcaggcac gacctggatg ggggaatgac tgggcgtcgt
      181 gggaccgtta tgaagctgat ctgaccaccc gctgatggag cctgaagtgt cgctaccccc
      241 gttggttgaa ccggccgaga gcctgactgt cgaagaggtc aggcgctact cgcggcactt
      301 gatcattccc gacgtcgcga tggtgggtca gaagcgcttg aagaacgcgc gggtgctcgc
      361 gatcggtgcc ggcggcctgg gttcgccggt gctcatgtac ctcgcggcgg ccggggtcgg
      421 aacgctcggc atcattgagt tcgatacggt cgacgagtcg aaccttcagc gccagatcat
      481 ccacgggcaa agcgacatcg gccgctcgaa ggcgcagtcg gcccgcgact cgatccacga
      541 gatcaacccg ctggtcaagg tcgtgctgca cgaagagcga ctcgagaacg agaacgtgtt
      601 cgagatcttc ggccagtacg acctgatcgt cgacggcacc gacaacttcg ccacccgcta
      661 cctcgtcaac gacgcgtgcg tgctgctcgg caagccctac gtctggggat ccatctaccg
      721 gttcgacggg caggcgtcgg tgttctgggc cgagaacggt ccgtgctacc ggtgccttta
      781 ccccgagccg ccgccgcccg gcatggtgcc gtcgtgcgcc gagggtggtg tgctcggcgt
      841 gctctgcgcg tcgatcgggt cgatccaggc gaccgaggcg atcaagctgc tgaccggcat
      901 cggcgacccg ctcgtcggtc ggctgatgat ctacgacgcg ctcgagatgt cgtaccggac
      961 ggtcaacatc cgcaaggacc ccgagtgccc gatctgcggc aagaatccga cgatcaccga
     1021 actcatcgac tacgagacct tctgtggcgt cgtctccgac gaggcagtgc tcgcggcgca
     1081 gggctcgacg atcgtgccgc gtgagctcaa gaagtggatc gacgacgggg agaacatctt
     1141 cctggtcgac gttcgtgagc cggccgagtt cgagatcgtg tcgattccgg gggcgacgct
     1201 catcccgaag ggcgagttcc tcaacggctc agcgctcgag cggatgccgc aggacaagcg
     1261 catcgtcttg tactgcaagt cgggcatgcg ctcggctgag gcgctcgcga tcgtgaaggg
     1321 cgctggcttc gccgacgccg tgcacgtcgg cagtggcgtg gtcggctggg tcaaccaggt
     1381 cgacccgagc ctgccctcgt actgaggcct cggactagca gcgtggagcg tgcgcgccgt
     1441 tctagccccc acggccctcc acgatcgcgg gacgtcgagg tgccgccgtt cgcccaacgg
     1501 gtgctcgatc tcgtcgacgc gataccaccg ggcagggtgc tcgcctatgg cgacatcgcc
     1561 gaatacctcg ctgagggcgg cccgcgccag gttggcaccg tgatgtcgac ttggggaggt
     1621 gccgtgccgt ggcaccgcgt cgtccacgcc gatggaacgc cggcacccag tcacgacggc
     1681 gaagtgctgc gactgctgcg ggccgacgca acaccgatgc gcggagaccg tgtcgacatg
     1741 acgcgcgccc ggtgggaccc cgactcctga gcgatgccga ggcggcatct tcacatccgg
     1801 tgtggttgct ctggggcgcg ttttgggcgg tatcggcgac cacacgggat gtgaagtcga
     1861 cggcggcagt gtcagcgttt cgtggtgtga tgcggtcgtg ccttcaagta acgggacgcc
     1921 gagtggtcag cgttcggccg acgggccagg cgcgcgtcag cccgccaacg tgcctcgcgt
     1981 cgttcgggcg ccagcgcgtg tcgttgtgcc ccccgtgctc gacgaggcgc aacgtcgtgc
     2041 ggtcgaacac cgcggtgggc cggtgctcgt gctcgccggc cccggcacgg gcaagaccac
     2101 cacgatcgtc gaggccgtgg tgcagcgcgt cgagtcgggt gagatcaccc ccgaccaggc
     2161 gttggtgctc acgttcagcc gccgggccgc tgccgaactt cgcgagcgca tcgccgcacg
     2221 cctcggccgc acgattcgtg atcccatcgc ccgcacgttc cactcctacg cgttcggtct
     2281 gttgcgcgca gatgcggctc gtcgcggtga ggccacgccg cggctgctgt caggtgctga
     2341 gcaagacctc gtcgtccgcg aactgcttcg aggtgacgtc gaggcaggcg ccgcgatgtg
     2401 gccgcagcgg ttgcatccgg cgttgctgac tcgaggcttc gcccaggagt tgcgcgactt
     2461 cgtgcaacgc gcggttgaac gtggcatcac gcctcgagag ctcgcacaga ttggccgccg
     2521 ttacaagcgc gtcgattggg tcgccgccgc gaagttcgcc gagcaatacg cgcaggtatc
     2581 ggcgctgcga gaggctgcgt cgtacgaccc agctgagctg atccgcgccg cagcgggttt
     2641 gttgaggtca gaaccccttg tgttgcaaga aattcgggct gctcacgagt tcgtcgtggt
     2701 cgacgagtac caagacacag acccggccca agaggagcta ctcgcactgt tggtcggcag
     2761 ccgcgaccta ctggcagtcg gagaccccga ccagtcgatc tatggcttcc gcggtgccga
     2821 cctcgaaggc gtgcggcgtt ttcccgatcg tttcaaggca cctgacgggt cggcgccgac
     2881 gatcgtcgcg ctcgacgtga gcaggcggtc gggtgcgaac ctgctggccg cgtcgcgtcg
     2941 tgtcgcgcag cgattgtctg gtctgcccgc gcatcgtgcc ttgcggcccg cgcccggcat
     3001 cgagccggga agcgccgaag tccatgtgtt cgcgacagcg aatgccgaag ccgcctacct
     3061 tgcgcaccgg cttcgcgagc tgcacctgtt gcacgacgtg ccgtgggccc gcatggcggt
     3121 gctcgtgcgc agtggcgtgc cactgcctgc gctgcgacgg gcgctccagt cggccggcgt
     3181 cccagtcgcc gcgtacgcgg aggagcagcc actggtcgac gtcccgattg tgcgggcatt
     3241 gttgcgagtt ctcgaagttg tgacgggtcg tctcgacttc gaaggcccgc aaggcgtcga
     3301 gctcgcacaa gagctcgtca ccggtccgct cggacgtgcc gacccgttgg cattgcgccg
     3361 gctccggcag gagttgcggt cgatggagtt ggcggcgggg ggtggtcgtg catcaggtgc
     3421 gctgctggca acggcgctga cgacgcccgc cgatctggtc ccgatcgagc gacgcattgc
     3481 cattcctgct ctacgcattg cggcggttct ccaggctggt cgcgccgcgg tcgcggagcc
     3541 gagtgccaca gccgaggccg tgctgtgggc gatctggcaa gcagcaggcc gcgcacctcg
     3601 cctcagttcc ctcgcactgt cgggcggccc ggggtccgct gccgccgacc gcgatctcga
     3661 tgcagcggtc gcgctgttcg acgccgctgc gcggtttgcc gaccggcttc ccaaggccgg
     3721 gccttcggtg ttcctcgatc acttgcttgg gcagcagatt cccggcgaca cgttggcgcc
     3781 acaggcaccg accgctgatg gcgtgcgact gatgaccgcg cacgccgcga aaggcctcga
     3841 gtgggacgtt gtggtcgttg cc
//
LOCUS       DASWVF010000775         5308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_200350, whole genome shotgun sequence.
ACCESSION   DASWVF010000775 DASWVF010000000
VERSION     DASWVF010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200350"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1743)
                     /locus_tag="VGD55_13715"
     CDS             complement(34..1743)
                     /locus_tag="VGD55_13715"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY0871452.1"
                     /translation="MVADREFIATDSATDSASADVASAAQAALAKAAARGTKHANLTA
                     GAGVAADPVTYLRRTWFDTFDWRLHKANLTLERIEQGRSKSVGGRLVLASHAGTGRPA
                     QTELVAPISADAPGSAVVRTAFPSDAVVPPGSMRIRLEPIVEMRALTPIITVRTVVRT
                     LRVRNDDDKTVARVVVEHAVGNDVGGSGGFGCRVSVVACRGYDAEALATARVIERLPG
                     LAATDTSVFAHAVAAIGRQPGDYSSKLDIALTVGMPTGYAMRRIMRALLDAAQRNVPG
                     VLADTDSEFLHDLRVAIRRGRSTLKVPGAALPAAQAAAAGAALKWLGDLTTPTRDLDV
                     YLLGLDDLTSEVTTPGWAVSQLDPFRDFLAARRAKEQAALVRSLRAVRSRRAFDAWAA
                     VSTGAELDDARDTTISEPVAPASEIVAKAAVLRAFKRVVKAGRSIDAGSPPTELHGLR
                     KRCKELRYTLEIFAALHDPAAQRALVDDLKKLQDCLGEFQDTDVQQHAIRQFAAQMLA
                     DDASNPTTVDAVMAMGRLADRLGERQVIARDTFATLFDRFAGPQPRAHLAALYERSTK
                     AQP"
     gene            1803..2120
                     /locus_tag="VGD55_13720"
     CDS             1803..2120
                     /locus_tag="VGD55_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY0871453.1"
                     /translation="MAIPTYAVVGDADGNPTDAACAHLVEIAQVTPSTTRGCEDCLRE
                     GTRWVHLRECLECGHVGCCDNSPRRHATAHFHATAHAIMRSIEPGEDWAWCFVDSLFL
                     EPV"
     gene            2133..3839
                     /locus_tag="VGD55_13725"
     CDS             2133..3839
                     /locus_tag="VGD55_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY0871454.1"
                     /translation="MEDEAASPHLRTHLHLDELLSELQTQLQAVIGTRDRVHALLDAV
                     LAIGTDLDLQTVLHRIVEAAAQLVDARYGALGVIDEVHLLSQFLTVGIDEETAARIGP
                     LPRGGGILGLLIDDPRPLRLHDIGEHPALFGFPAHHPPMKTFLGVPIRVRDEVFGNLY
                     LTEKKGGGDFDDEDESVVLALAAAAGVAIENARLYDDVRHRERRLNASAEVTTALLSG
                     TEPDDVLRLVAARARDLAEADFAAIALPLGDTLVIEVADGDAADHVTGKRIDPEASLI
                     GDAFRGGVTVAIDDIHQSPHWPTNSEALNEFGPVIMVPLMANGARLGVLWVANRIGGR
                     HFGAAHQSMLEAFADQAALGLELARQRRETEQLSLFRDRDRIARDLHDTVIQRLFATG
                     MQLEGSMRYMTGPEASDRVQGAVSDLDQTIKEIRSTIYALQRSDRSPSSSLRARIVEL
                     IEELTPSLGFTPTLRLEGLVDTRVSKEVAHNLLPVLREGLSNTARHAKATHADVSAVV
                     DDDCVTLTVSDNGVGLAPGGRRSGLANLEARAGSLGGTFLARSAPEGGTELVWRVPVG
                     DD"
     gene            complement(3885..4274)
                     /locus_tag="VGD55_13730"
                     /pseudo
     CDS             complement(3885..4274)
                     /locus_tag="VGD55_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            4399..5073
                     /locus_tag="VGD55_13735"
     CDS             4399..5073
                     /locus_tag="VGD55_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0871455.1"
                     /translation="MPTTEPQITVFLLDDHEVVRRGVKELLESEPDIVVIGEGSTAAE
                     ALARVPALRPQVAVLDVRLPDGDGVSVCRDLRSKMPDLACLMLTSFADDDALYDAILA
                     GAAGYVLKQIKGSDLVNAVRTIAAGGSLLDAKTTARIMQRLRDDAADREKADPFAALT
                     SQEHRILDLIGEGLTNRQIGERMFLAEKTVKNYVSSMLSKLGLERRTQAAILATESKQ
                     DKHVNP"
     gene            complement(5063..>5308)
                     /locus_tag="VGD55_13740"
     CDS             complement(5063..>5308)
                     /locus_tag="VGD55_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY0871456.1"
                     /translation="MPGNAVAGHANVLIFPDLNTGNTTYKAVQRSAGAIAIGPVLQGL
                     RRPVNDLSRGCTVADIVNTVAITAIQAQQLQGAPVKD"
BASE COUNT          945 a         1770 c         1711 g          882 t
ORIGIN      
        1 cgccaccctt gatgttgtag gtcgcgaaaa ccctcacggt tgagctttcg tgctgcgctc
       61 gtacaacgcc gcgagatggg cacgaggctg cggcccggcg aagcgatcaa acagcgtggc
      121 aaatgtatcg cgcgctatca cctggcgttc acccaatcga tcggcaagcc gacccattgc
      181 catgacagcg tcgacggtcg tggggttcga cgcatcgtcg gcgagcattt gcgctgcgaa
      241 ctgtcgaatc gcatgctgct gcacgtcggt gtcctggaat tcaccgaggc agtcttgcag
      301 tttcttcaga tcgtcgacga gcgcgcgctg ggcggcgggg tcgtgcagcg cggcgaatat
      361 ctccagcgtg tagcgcagtt ccttgcatcg tttgcgaagg ccgtgcagct cggtcggtgg
      421 cgaaccggcg tcgatactcc tgcccgcctt cacgactctc ttgaacgcac gcaagaccgc
      481 ggcttttgcg acgatctcgc tggccggcgc aaccggctca gatatcgttg tatcgcgcgc
      541 gtcgtcaagt tcagctccgg tgctgaccgc cgcccacgca tcgaaggcgc gccgcgaacg
      601 gactgcgcgc aacgagcgca ccaaagccgc ctgctccttc gcccgacgag cggcgaggaa
      661 gtcacggaat gggtcaagct gcgagacggc ccacccaggc gtggtcacct cactcgtcaa
      721 atcgtcgagc ccgagcaggt agacgtcgag gtcacgagtg ggcgtcgtca aatcgccgag
      781 ccatttcaac gcggcaccgg cggccgctgc ctgcgccgcc gggagggccg cgccagggac
      841 cttgagtgtc gaacgccctc gtcttatggc gacgcgcagg tcatgcaaga actcgctgtc
      901 ggtgtcggca agcacgcctg gcacgttgcg ctgagccgcg tcgagcaggg cgcgcatgat
      961 gcgccgcatc gcgtacccgg tgggcatgcc aacggtcagg gcgatgtcga gcttgctcga
     1021 atagtcgccg ggctgccgac cgatcgccgc gaccgcgtgc gcgaacaccg acgtgtcggt
     1081 ggcggcgagg ccaggcagcc tctcgataac tcgcgcggtc gccagggctt cggcgtcata
     1141 gcccctgcac gcgacaacgg atacccgaca gccaaaacca ccggagccac cgacgtcatt
     1201 tccaacggcg tgctcgacga caacacgcgc gacggtcttg tcgtcatcgt tgcgaacccg
     1261 aagtgtgcgg acgacggtcc gcaccgtgat gatcggagtg agcgcacgca tctcgacaat
     1321 cggctcgagg cgaatccgca tggatcccgg cggcacgacc gcgtcagacg ggaacgcggt
     1381 acgaaccact gcggacccgg gtgcatcggc agagatcggc gcaaccagtt cggtctgcgc
     1441 tggccgaccg gtacctgcgt gcgaggcgag caccaaccgc cctccgacgg atttcgatcg
     1501 gccctgctcg atgcgctcga gggtcaggtt cgccttgtga agtctccagt cgaaggtgtc
     1561 gaaccaggtc cggcgcaggt acgtgacggg gtctgctgcc acgccggcac cagccgtgag
     1621 gttcgcgtgc ttggtcccac gtgcggccgc cttggccagc gcggcctgcg ccgccgacgc
     1681 cacgtcggcc gatgcggaat ccgttgcgct gtcggtggcg atgaattcgc ggtcggcgac
     1741 cattgtcagg gaaggttacc ctgggtaagc cccaactcag gcgacccgaa gggacaacgc
     1801 gaatggcgat ccccacctat gccgtcgtcg gagatgccga cggcaatcca accgacgcgg
     1861 catgcgccca cttggtcgag attgcgcaag tcacgccgtc gacaacgcgt ggctgcgaag
     1921 actgtcttcg cgagggaacc cggtgggtgc acctgcgcga atgcctcgag tgcggtcacg
     1981 tcggctgctg cgacaactca cctcggcgcc acgccacagc gcattttcat gcgaccgctc
     2041 atgcgatcat gcgatccatc gagccgggcg aagactgggc ctggtgcttc gtcgactccc
     2101 tcttcctcga gcccgtgtag caggatggcg atgtggaaga cgaagcggcc agccctcatc
     2161 tgcggaccca cctgcatctc gacgaactcc taagcgaact tcagacccaa ctccaggcgg
     2221 tcatcggcac ccgcgatcgg gtgcacgcgc tgctcgatgc cgttctcgcg atcgggaccg
     2281 acctcgacct ccagaccgtg ctgcaccgca tcgtcgaggc cgccgcccag ctcgtcgatg
     2341 cccgctacgg agccctggga gtgatcgacg aggtccatct gctctcccaa ttccttacgg
     2401 tcggcatcga cgaagagact gccgcgcgca tcggtcccct cccccgcggc ggggggattc
     2461 tcggcctgct gatcgacgac ccgcgaccat tgcggcttca tgacatcggc gaacatcccg
     2521 cgttgttcgg tttcccggcc caccaccccc cgatgaagac ctttctcggc gtgccgatcc
     2581 gcgtgcgcga cgaggtgttc ggaaacctct atctgacgga gaagaagggg ggcggcgact
     2641 tcgacgacga agatgaaagc gtggtcctcg ccctagccgc agccgcaggc gtcgcgatcg
     2701 agaacgcacg cctctacgac gacgtccgac atcgcgaacg gcggctcaat gcctccgccg
     2761 aggtcacgac tgcgctgctc tccgggaccg agcccgatga cgtcctgcgc ctcgtcgcgg
     2821 cccgcgccag ggatctggcc gaggccgact tcgccgcgat cgcgctacct ctcggcgaca
     2881 cccttgtgat cgaggtcgcc gacggagacg cggctgacca cgtcacgggg aaacgaatcg
     2941 atcccgaggc atccctgatc ggcgacgctt ttcgcggtgg tgtcacggtc gcgatcgacg
     3001 acatccacca aagcccgcac tggccaacca actccgaggc gttgaacgag ttcgggccgg
     3061 tcattatggt gccgctcatg gccaacggcg cgcggctcgg tgtgctgtgg gtcgccaacc
     3121 gaatcggcgg tcggcatttc ggtgcagcgc atcagtcgat gctggaagcg ttcgccgacc
     3181 aggcggccct cggactcgaa cttgcgcgtc aacgtcgaga aaccgagcag ctcagcctct
     3241 tccgcgatcg cgaccggatc gcgcgggacc tgcatgacac tgtgatccag cggttgttcg
     3301 cgaccggcat gcagctcgag ggctcgatgc gttacatgac gggccctgag gcatcggatc
     3361 gcgtgcaggg tgcggtgagc gacctcgacc agaccatcaa ggaaatccgc tcgacgatct
     3421 acgcactcca acgatccgat cggtcgccct cgagcagctt gcgggcccgc atcgtcgagc
     3481 tgatcgagga actcacaccc tcccttgggt ttactcctac cttgcgtctt gaaggcctcg
     3541 tcgatacccg ggtctctaag gaggttgcgc acaacttgtt gccggtgcta cgcgaaggct
     3601 tgtcgaatac cgcgcgccac gccaaggcga ctcacgccga tgtgtctgcc gtcgtcgacg
     3661 acgactgtgt cacgctcacc gtgagcgaca acggagtcgg gctagctccc ggtgggcgca
     3721 ggagtggtct tgccaacctc gaagccaggg ctggcagtct cggcggcacc ttcctcgctc
     3781 gctccgcgcc cgaaggtggc accgaactcg tctggcgagt gcccgtcgga gacgactgac
     3841 gctgcgccga tcgaccacgc cagcgcgggg atccgtcggc ccgagacgag ttcaggtcgc
     3901 aagcgaagat agtgatcgga cgccgcggga gcccaggtcg gcagctccag tgcggtgagg
     3961 cgagcgacct cggcatcggc caccacacgg tcgatgcgac ccgttaccac aacggtccaa
     4021 ccagatccgg tcgtagcaat gtcgtcgact tcgaaggcga ccacgctgcc tgaagcatgc
     4081 gttgcggggc cggagcggcg ggcggtgcgg atcacgatgt cggttgcgtc gagggtgaac
     4141 gcgacgggga ccacgaaggg cagcgcgcga tgtgaataca acagccgccc cacagctgcc
     4201 gttgcaatga gggccagcga ctcctcatcg gacagcgctt cgtttcctga cgcgtcggcg
     4261 ggcatgctct ccatagtcgg cgtcgctcgg cagtggccgc agagtcctag gtcccgtcgc
     4321 gccgggacac gccggagaag ctacggtgga cgcacgcccc aggagcagac aggggtaggc
     4381 gactgattgg gaggtctggt gccgacgacc gaaccccaga tcaccgtgtt cctgctcgac
     4441 gaccacgagg ttgtccggcg cggagtgaag gagctcctcg agtccgagcc cgacatcgtc
     4501 gtgatcggcg aaggatccac cgcagctgaa gcgttggcgc gggtgcccgc gctacgcccg
     4561 caggtcgccg tcctcgatgt tcgattgcct gatggcgacg gggtcagcgt gtgccgcgac
     4621 cttcgctcga agatgcccga cctcgcgtgc ctgatgctca cgtcttttgc cgacgacgac
     4681 gcgctgtacg acgcgatcct cgccggtgcc gccggctacg tcctgaagca gatcaaaggc
     4741 agtgacctgg tcaacgcggt gcgcacgatc gcggccggcg gttcgctgct cgacgccaag
     4801 acgacagctc ggatcatgca acggctgcgc gacgacgcgg cagatcgcga gaaggccgat
     4861 ccgttcgccg ctctcacaag tcaagagcac cgcatcctcg acctgatcgg cgaaggcctg
     4921 acgaacaggc agatcggcga gcggatgttc cttgccgaga agacggtcaa gaactacgtc
     4981 tcgtcgatgc tcagcaagct cgggctcgag cgccgtaccc aggccgcaat ccttgccacc
     5041 gagagcaagc aggacaagca cgtcaatcct tgacgggtgc cccctgcaat tgctgagctt
     5101 gaatcgcggt aatcgcgacc gtgttgacga tatcggccac cgtgcatcct cgtgatagat
     5161 cgttgaccgg acgccgaagg ccctgcagga ccggcccgat cgcgatcgca ccggccgagc
     5221 gctgcaccgc cttgtaggtc gtgttccccg tgttcaggtc gggaaaaatg agcacgttcg
     5281 cgtggccagc caccgcattg cccggcat
//
LOCUS       DASWVF010000776         4839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_98428, whole genome shotgun sequence.
ACCESSION   DASWVF010000776 DASWVF010000000
VERSION     DASWVF010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98428"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VGD55_13745"
     CDS             complement(<1..778)
                     /locus_tag="VGD55_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEY0871457.1"
                     /translation="MTATEAERIHAYWRAANYLSVGQIYLLDNPLLRQPLTIEHVKPR
                     LLGHWGTTPGLNFVYAHMNRVIKRDDLDAIYITGPGHGGPGLVANSYLEGSYTETYPA
                     ITKDAEGMRRLFRQFSFPGGIPSHVAPETPGSIHEGGELGYALVHAFGAAFDNPNLLV
                     CCVVGDGEAETGPLATSWHSNKFLNPKTDGAVLPILHLNGYKIANPTVLARIPPEELE
                     SLLRGYGYTVHTVEGDDPKLVHQQLAATMEAAVGEIRAIQR"
     gene            949..1473
                     /locus_tag="VGD55_13750"
     CDS             949..1473
                     /locus_tag="VGD55_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0871458.1"
                     /translation="MTTVPPVAAALADHPFLSTLPSQSLRRLATHVQSRIYAAGEAIL
                     REGEVANRFFLIRHGSVRLDIEVPGRGPVEIETLGADSALGWSWLFQPHRWQLSATAV
                     TRTSTLVFDADALQVVMASDPALGYELMRRFAGVMFDRLNATRLRLTSDAEIPFAGTA
                     GPWAGKRLTSPHWQ"
     gene            1702..2136
                     /locus_tag="VGD55_13755"
     CDS             1702..2136
                     /locus_tag="VGD55_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0871459.1"
                     /translation="MSADSSGLSTLSEGDALALLATVSVGRLVYSDRALPFVSPVNFI
                     LDGPDVIVRTGRRSRLATHTPGNIVAFEVDEIDAVDRSGWSVVVTGRVQLIDDVSEVA
                     RLAAWHLQTWQPATTDRYLRLRPEMVSGQHLPAANAYSYPGA"
     gene            2346..2936
                     /locus_tag="VGD55_13760"
     CDS             2346..2936
                     /locus_tag="VGD55_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871460.1"
                     /translation="MRRKTFDALMTTGGLVLAAVLLVAGVLGLWAHNFIDNQVKSQLS
                     QQQIFFPAAGSAAISDPLIKPYLTKYGGQQMTNGAQAEAYADHFIAVHLLKIGGGKTY
                     SQLSAQAQTDPTNTTLANTVNTMFKGETLRGLLLNGYAFWKMGQFALMAAVISFIGAA
                     FMLLLAGLGIVHLRRTDPAAELLPKLGARTPAPVEP"
     gene            3055..>4839
                     /gene="pta"
                     /locus_tag="VGD55_13765"
     CDS             3055..>4839
                     /gene="pta"
                     /locus_tag="VGD55_13765"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY0871461.1"
                     /translation="MPDALYVTSTQPRSGKAVVALGLMELLTRQVHRIAVFRPVIAPG
                     AARDPLIELLRERYLLDIDYDDAYAFTYSDAARIESAGGTSRLIALIADHFARLRDKF
                     EFVLCLGTDYTGPSAATELALNAELAVNLGTPVVNVVSGYGKDAESLSIASRSSQELL
                     VDHGCAVAATILNRVEPAVVVQLRAAPPVAGQAPVYLLPDLPVLSALTIDEVVSALGA
                     TVLAGYGAPMHREVEAYLAGSGYLQTMLPRLVNGTLLFASGDRADLAVAMGAAALSPS
                     LPTPSGIVLTCGIKLDAATLALLEPSGIPVVAVEADTYAALHTLEGMRGEIRPGSRRK
                     IAAALGQFARSVDGDELTARIRLTRSDVVTPLMFSAGLLERARSNRRSIVLPEADDDR
                     VLRAAEELVHQNVVSVVLLGDPTAVAARVEQLGLNLADTEVIDPATSQLRQQFADAYA
                     VLRAHKGVTPAAAWDLMANSTYFATMLVHTGRVDGMVSGATHTTAETIRPALEIIKTA
                     AHGAKVSSAFFMCLPHRVLVFADCAVNLDPNAAELADIAVRTVETAAAFGIEPVVALV
                     SYSTGTSGTGVGVEKVRAATELVAALRPD"
BASE COUNT          776 a         1564 c         1574 g          925 t
ORIGIN      
        1 gccgctggat ggcgcggatc tcgccgacgg ccgcctccat ggtcgcggcg agctgctgat
       61 ggaccagctt cgggtcgtca ccttcgacgg tgtggaccgt gtacccgtag ccgcgcagca
      121 gcgactccag ctcctcgggc gggatccggg ccaggaccgt cgggttggcg atcttgtagc
      181 cgttcaggtg caggatcggc aacaccgcgc cgtccgtctt ggggttgagg aacttgttcg
      241 agtgccaact cgtggcaagc ggcccggttt cggcttcgcc gtcgccgacg acacagcaca
      301 ccagcaggtt cgggttgtcg aacgccgcgc cgaacgcatg gacgagcgca tacccgagct
      361 cgcctccctc atggatcgag cccggcgtct caggtgcgac gtggctcggg atgccgcctg
      421 ggaacgagaa ctgacggaac agccggcgca taccttcggc gtctttcgtg atcgccgggt
      481 aggtctcggt gtagctgcct tcgaggtagc tgtttgcgac gagcccggga ccgccgtggc
      541 ccggaccggt gatgtagatc gcgtcgaggt cgtcgcgctt gatgacccgg ttcatgtgcg
      601 cgtagacgaa attcaggccg ggcgtcgtgc cccagtggcc gagcagtcgc ggcttgacgt
      661 gctcgatcgt gaggggctgt cgtaacagcg gattgtcgag cagatagatc tgcccgacgg
      721 agagatagtt cgcggcccgc cagtacgcat ggatgcgctc ggcctcggta gcggtcaagg
      781 gtccagtcgc tagatcggaa gtcgtcgaca tccggggtcc tctcctgccg tggtcagtgc
      841 ttgagtcaac cctcgcatgc ccggtcggtg accgggggtg ccgatagtcc cgggctgtcg
      901 tgacgtccag ccctcggcgt gcgcgctgcc gtcgggtgca ctaagtacat gaccaccgtc
      961 ccgccagtcg ccgccgcgtt ggccgatcac ccgttcctct ccacgttgcc gagtcagtcg
     1021 ttgcgcaggc tcgccacgca cgtgcagtcg cgcatttatg cggctggcga ggcgatcctg
     1081 cgtgaggggg aggtcgccaa tcggttcttc ctcatccgcc acggctctgt gcggctcgac
     1141 atcgaggtgc ccggtcgggg cccggtcgag atcgagactc ttggcgccga ctcggccctg
     1201 ggctggtcgt ggctgttcca gccgcaccgc tggcaactca gcgccaccgc ggtcacgcga
     1261 acgtcgactc tcgtgttcga tgcggacgca ctccaggtgg tcatggcctc agacccagca
     1321 cttggctacg aactcatgcg gcgcttcgcg ggagtcatgt tcgaccggct caacgcgacc
     1381 cggctccggc tgacgagtga cgccgagatc ccgttcgcgg gcacggcggg cccgtgggcg
     1441 ggcaaacgtc tcaccagccc ccactggcaa tagcggtaga gggcttatga ggacgtcatg
     1501 ttgacgatcc gcgcgttgcc acgcgcgctg cgttcgcagg cctctacaag actcacagtg
     1561 ctccgggcaa tcgaatggcg gtatggcaca ggtcgcgtgc acacgggact gatggctcgg
     1621 gttcgcgcaa ggagtcaaat cccagtcaat ggggactttg gccccttcgg atcgtggcga
     1681 tccggggatg agcattgacg tatgtccgct gactcgtcag gccttagcac gttgtccgag
     1741 ggtgacgctc ttgcgttgct cgcgaccgtg tccgttgggc ggctggtcta ctccgaccgc
     1801 gcgttgccgt ttgtctcccc ggtcaacttc atcctcgatg gcccggacgt catcgtccga
     1861 acgggccggc gatcgcgcct cgcaactcat acgccgggca acatcgtggc attcgaggtc
     1921 gatgagattg acgccgtcga tcgctcaggt tggagtgtcg tcgtaacagg tcgcgtgcag
     1981 ctcattgacg acgtttcaga ggtagcccgc cttgccgcct ggcatctgca aacctggcag
     2041 cccgctacga cagacaggta cttgcgactt cgaccggaga tggtcagtgg ccagcacctg
     2101 ccggccgcca acgcgtattc ctacccaggg gcgtaagcag cctttgcggg tgttcggccg
     2161 ccaccacgag gccgtcttcc acatctcccc agcgctgctg agtcccggtc aactgaggtc
     2221 tatggcatcg cgggtgcggg acctaagtcg ctacgccgca gcggcagccg cagacaacca
     2281 tcggtattgc aaggttcgac ggatcacatc agccggatcg tccggctcgt aagaaggagg
     2341 tcgtcatgcg ccgcaagact tttgatgcac taatgactac aggaggcctc gttctcgcgg
     2401 ccgtcctgct cgtggcgggt gtgttgggtc tctgggccca caacttcatc gacaaccagg
     2461 tcaagtccca gttgtcgcag cagcagatct tcttcccagc ggcgggttcg gcagcgattt
     2521 ccgacccgct gatcaagccg tacctcacca agtacggtgg ccagcagatg acgaacggtg
     2581 cgcaggctga ggcctacgcc gaccacttca tcgctgtcca cctgctgaag atcggtggcg
     2641 ggaagaccta ctcccagctg tcggctcagg cacagactga cccgacgaac accacactcg
     2701 cgaacaccgt caacaccatg ttcaagggtg agacgttgcg tggcctcctc ctgaacggat
     2761 acgcgttctg gaagatgggc cagttcgccc tcatggcggc cgtgatctcc ttcatcggag
     2821 cagccttcat gctgctgctc gccggtctgg gaatcgtcca cctgcgtcgg accgatcctg
     2881 cggcggagtt gctcccgaag ctcggtgcgc ggaccccggc tcccgtcgaa ccgtaacggc
     2941 gaccgcaccg acgatgaggg cccctgcttc ggcaggggcc ctcatgccgt tgtaacgcag
     3001 tactgatgta gtgagaactg atgtcgtggg acggcgattg gtggaggagg gaacgtgcct
     3061 gacgctctgt acgtgacatc gacgcagccg cgttcgggta aggccgtagt cgcgcttggg
     3121 ctcatggagc tactcacccg gcaggtccat cggatcgcgg tcttccgccc ggttatcgca
     3181 cccggcgccg cgcgcgaccc gttgatcgag ttgttgcgtg agcgctatct gctcgacatc
     3241 gactacgacg acgcctacgc cttcacgtac agcgacgctg cccgcatcga gagtgctgga
     3301 ggcacctcgc gattaatcgc gctgatcgcc gatcacttcg cccggctccg tgacaagttc
     3361 gagttcgtcc tgtgccttgg caccgactac accggcccat cggccgcaac cgaactggcg
     3421 ctgaacgctg aactggctgt caacctcggg accccagtcg tgaatgtcgt ctccggctac
     3481 ggcaaggacg ccgagtcgct ctcgatcgcg agcaggagct ctcaggagct gctcgtcgat
     3541 cacggctgcg ccgttgcggc gaccattctc aatagggtcg agccagccgt cgtcgtgcag
     3601 ttgcgggcgg cgcctcctgt tgccggacag gcgcccgtct acctgcttcc cgacctgccc
     3661 gttctctccg cattgacgat cgacgaggtc gtcagcgcac tcggcgccac ggtgctggcc
     3721 ggttacggtg cgccgatgca ccgcgaggtc gaggcctacc tcgcaggatc gggctacctg
     3781 caaacgatgt tgccaagact cgtaaacggc acgttgctgt tcgcttccgg cgaccgggcc
     3841 gatcttgccg tcgcgatggg tgcggctgcg ctcagtcctt cgttgcccac gccgtccggc
     3901 atcgtgttga cgtgcggtat caagctcgat gccgcgacac tcgcgttgct tgagccgtca
     3961 ggcattccag tcgtcgcggt cgaggctgac acttatgccg cgctgcacac cttggaaggc
     4021 atgcgcgggg agatacggcc aggtagccgg cgcaagatcg cggctgcgct cgggcagttt
     4081 gccaggtccg tcgacggcga cgagctcacc gcgcgtatca ggctcacccg aagtgacgtt
     4141 gtgacgccgc tcatgttctc ggctggtctg ctcgagcgag ccagaagtaa ccggcgctcg
     4201 atcgtgctcc ctgaggccga cgatgatcgg gtcctgcgtg ccgccgagga actcgtgcat
     4261 cagaacgtgg tttcggtggt gctgctcggc gaccccactg cggtcgcggc gcgggtcgaa
     4321 cagctcggat taaatctggc cgacaccgag gtgatcgatc cggccacatc gcagttgcgg
     4381 cagcagttcg cggacgccta cgccgtgttg cgcgcccaca agggcgtgac gccggccgcg
     4441 gcgtgggatc tcatggccaa cagcacctac ttcgcgacga tgctggtgca cacaggtcgc
     4501 gtcgacggca tggtctctgg tgcgacacac acgacggccg agaccattcg gccagctctc
     4561 gagatcatca agactgcggc gcatggcgcc aaggtgtcga gcgcgttctt catgtgcctg
     4621 ccgcatcggg tacttgtgtt tgccgattgc gcggtcaatc tcgatcccaa cgcagcagaa
     4681 ctcgccgaca tcgcggttcg caccgtcgag accgctgccg cgttcggcat cgagccggtc
     4741 gtcgcgctgg tctcgtactc cacggggacg tcgggcaccg gtgtcggtgt cgagaaggtg
     4801 cgtgcagcga cggagttggt cgcggcgctg cggcccgat
//
LOCUS       DASWVF010000777         9080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_149316, whole genome shotgun sequence.
ACCESSION   DASWVF010000777 DASWVF010000000
VERSION     DASWVF010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149316"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VGD55_13770"
     CDS             <1..792
                     /locus_tag="VGD55_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871462.1"
                     /translation="GATFPGMGAGHGFGALVGQFYGGPHKVCVYAINAGGGVNPALGC
                     ATVTLPAGNPKGSLNIAQGLPGAIRVVGWALDPDTSMPITTHVYVDGHWTGAATANLT
                     RPDVASAFPGYGAGHGFDVTVPVVGGGQHQVCTYAINVGHGSANPKFGCATVTMPTGP
                     PGGTLDIVQGQSGGVRIAGWSIDPDTTGPVQVHVYVNGVWTAAVTANTSRPDVGSAHP
                     GYGDQHGFDTTVPLAAGVHTVCVYAINVGPVAPNPKLGCATVTSS"
     gene            complement(779..1879)
                     /locus_tag="VGD55_13775"
     CDS             complement(779..1879)
                     /locus_tag="VGD55_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0871463.1"
                     /translation="MRVALDATPLLGTRTGIGHYVAQLSAALANEADIDVVLTAFTWR
                     GQQHLREIEAAGVDVHSRRAPARLLRAAWGHSNFPPVEMFSGNVDVFHGTNFVLPPTR
                     RAAGVVTVHDVSFVLHPETVDSTSLAYQHLVPRALDRARVIITPSNAAADDLVEVYGT
                     NRDRIVVTPLGVDEAWAAATPPSDKWLQTHGIPQRYLLFVGTLEPRKNVHDLVQAHRL
                     LRRDDPSIPMLVLAGAASSSSAVAFNPDDVVWPGYVRQGDLRSLVAGAAALALPSRYE
                     GFGLPALEALACGVPVVTTDIPVLREVTGGLAAYVPVGDIPALAEALRRMLATEHDAA
                     AVAKRRAWAATFTWERCASATVAAYRRALTTR"
     gene            1996..2823
                     /locus_tag="VGD55_13780"
     CDS             1996..2823
                     /locus_tag="VGD55_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871464.1"
                     /translation="MSPPAVTQMAEMWRYRGLIGNFASRELKSKYKHSLLGWTWSLIN
                     PAATLAVYSIVFSTIFRALPPKAHNGMTTYVIYLFIGLVAWNFFSNVVNGSMAALIGA
                     GPLLKKIYFPPYAAVLGNSLSALVQTFIEAGVLLVVLLAAGNASWTLLLAPVVLALLL
                     VFASGIGLLFALFNVFYRDVSYIVTVILQLLFYATPVIWAPSQLLGPTHKHGEFAVHV
                     LSYNPMFQYVEAFRDALWEGHVPSLSRWAWMLGGAVVSITIGLMVFIRFSRDVSEEL"
     gene            2820..4061
                     /locus_tag="VGD55_13785"
     CDS             2820..4061
                     /locus_tag="VGD55_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871465.1"
                     /translation="MTLLHPDSRPPAVVVDHVTKEFKLYKDRRTSLKERFTKRRPSRY
                     DMFRALDDITLNVPAGTTYGLIGSNGSGKSTLLKLMANIHRPTSGTVTHAGRISALLE
                     LGAGFHPELSGRDNVYLNGSILGMTRKQVDAAMDAIIDFSGIEEFIDTPVKVYSSGMY
                     VRLGFSIAVNLDPEILIIDEIIAVGDAEFQRRCFDYLYELRKRGVTIIFVSHSLPLVQ
                     TLCDRAAWIEKGVLSAEGPAMKVVDEYIATVNKAERERFEAEGHGRADGDTQRRGDGE
                     ATVTGLTFLDASGEPTLAVVAGERVTLRLSYEATTPVDDPVFGLGFQTENGVLVSGPN
                     SQFGGVCLGRIYGRGYIDYVLDPLQLTPATYLVSTAITDSTLLHVYDYRDRAFTLAVQ
                     PGKSPDRNGLVTLDGHWVLPT"
     gene            4102..7932
                     /locus_tag="VGD55_13790"
     CDS             4102..7932
                     /locus_tag="VGD55_13790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0871466.1"
                     /translation="MRVNLGCGQAYMEGWTNVDASPDVKADIHLDAAEFVRQYGDQVD
                     EVYMGHVMEHLMPGYASSLLRLLNERLRPGTVISVVTPDMRAIFEAYLSGEIDNDKLN
                     ASFIYSYVQPSHHVWCYDEATLLELFRRAGLQNAEPISDPTIYPPVFHKVGPESQWQC
                     GVRALAVGHEAPPFEVEVNELDGIDRTSHISSNVEAVTPEHELLNRIENLRASLIREY
                     ERRKALEARLTVSPEETTAPEAGVKPVEPPVPVSTVGGRREVAAAPTEVADESAPSLL
                     SPAGAKARAFDVVKRTLPQGSKSRNIVRGGLLAYRESRRVSRDVRALLAESGVVEPHE
                     PSYYTWYRRHRATTAEHKAQRDASAAASQPLHVLVIVFTGPEENGNATATVESIRKQT
                     WQHWDVEVCADAGHAPDIKGEHIRVREGDTRVTAAHYAIAESDADFVILLDAGDRLVR
                     ECLYQVARAAWQDPLVDLVTWDDDLLGRDGRRHDPRFRPEWSPESMVGAGYLGQSFAI
                     RRRRALFAGNLRNTYGGATRWDLLLRSDLNAERVVRVPRVLAHVRQRRQGINPDGVRV
                     VQEALDSRGVPAVAELADQSVRLRWKLENWPHVTIVIPTRHNQRMLSKVLPGLAATDY
                     PSFDVRIVDNGGQTADHEAWYAQNTAGLDLSVQWWTETPFNYSRVNNAAAAGALGEVL
                     VFLNDDIELPDSSWLREIVGWATQPEIGVAGLQLTRADGKIQHAGVILGMGGFADHVF
                     EGMSPGSDSLLGPTDWYRNVLAVTGACLAIRRETFDAVGGFDERFILCGSDVALGLDT
                     TQLGLRNVCSPFGGVRHLESATRGSQIPTEDFFASYWRYNTWLFGGDPYFSPSVSLSS
                     RKPKLVGLQEPTPAQRLSSPLGRDLQVFRQRSDAAESTMLADTCRVNDVDVAAIKNLH
                     ASNAAHLDVRTVNWFIPDIDSPFYGGINTALRLADYLHRNHGVQNRFVVWGSGPDYYV
                     RSAITAAFPSLADSEISFFDGPDSASLELVPPADASIATLWLTAYAVARAQNTKRKFY
                     LVQDFEPMFYPASTPYALAEESYRLGLYGLCNTDNMRQIYVDDYQGRAMSFSPAVDPT
                     VFHANNRPFRSDDSPATVFVYARPGHWRNCWELASLALREVKERLGDQVRIVTAGSWA
                     TDPDAADAIKQLGLLPYKATGELYRHCDVGLALTVSKHPSYLPLELMACGVPVVAFDN
                     PWGYWILKDGENSLLARRTVPGIVDALERACLNRTLRKELAAHAVADIAARHGSWDAA
                     FRGIYDYLCDPEGH"
     gene            7938..>9080
                     /locus_tag="VGD55_13795"
     CDS             7938..>9080
                     /locus_tag="VGD55_13795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0871467.1"
                     /translation="MVSGSAVRRRVGVVTTEPLTEQLAGPAIRAVELGRVLATEHDVE
                     VVSTALCSGPGPEGNWRYADVAALRELAGRCDVVVLGGDVLAANPWLTDAGPAIVVDL
                     YDPFHLEQLEQARDLGEDERRRVVFGCIDVLNTQAVLGDTFLCASTRQRDMWLGHLAA
                     CGRVNPATYDADPTLRSLLRIVPFGVAAEPPIAGAPALRGVVDGIGVDDEILLWGGGI
                     YNWFDPKTLIRAVDALRISRPKLRLVFMGVQHPNPVVKTMRAAAEAWELSNELELTDV
                     HVFFSDSFSSGWIPYAERGRYLAEATIGVSTHHDHVETAFSFRTRILDYFWAGLPVVT
                     TRGDALAEVIERAGAGRTVEPGDVDGLAAAINDLLDDEVARSSAAAA"
BASE COUNT         1530 a         2952 c         2935 g         1663 t
ORIGIN      
        1 ggcgctacct tccctgggat gggtgcgggg cacggcttcg gcgcactcgt cgggcagttc
       61 tacggcggcc cgcacaaggt ctgtgtctat gcgattaacg cgggcggtgg cgtgaatcca
      121 gccttgggtt gcgccacggt caccttgccc gctggaaacc ccaagggctc gctgaatatc
      181 gcccaagggc tgccgggtgc gatcagggtt gttggctggg cgcttgaccc cgacacctcg
      241 atgccgatca cgacccacgt gtacgtcgat gggcactgga ccggcgctgc gacagcgaac
      301 ctcactcggc ctgacgtggc atcggcgttc cccggctacg gcgcagggca cggcttcgac
      361 gtcacggtcc cggtcgtggg tggtggtcag caccaggtgt gcacctacgc catcaacgtc
      421 gggcacggca gcgccaatcc gaagttcggc tgcgcgaccg tgacgatgcc gactggcccg
      481 ccgggcggca cgctcgacat cgtgcaaggt cagtccggcg gagtgcggat cgccggctgg
      541 agcatcgacc ccgatacgac gggtcctgtg caggtgcacg tatacgtgaa cggggtctgg
      601 acagcggccg tcaccgcgaa cacctcgcgc ccagacgtcg gctcggcgca ccccggatac
      661 ggcgatcagc acggattcga caccaccgtg ccgctagccg cgggcgttca caccgtgtgc
      721 gtgtacgcga tcaacgtcgg tccggtcgcg ccgaacccca agcttggctg cgccacggtc
      781 acctcgtcgt aagcgcgcgc cggtacgcgg ccaccgtcgc ggaagcgcag cgttcccagg
      841 tgaaggtcgc ggcccatgcc cgacgtttcg ccacagctgc tgcgtcgtgc tccgtcgcga
      901 gcatccggcg tagtgcttcc gccaacgcgg ggatgtcgcc gacgggcacg tacgcggcga
      961 ggccgccggt cacctctcgc agcaccggga tgtcggtcgt caccacgggt acgccgcacg
     1021 cgagcgcctc taacgccggc agcccgaaac cctcgtaccg cgagggcaaa gccagcgccg
     1081 ccgcacctgc taccagcgac cgcaggtcgc cctgccggac gtaaccgggc cacaccacgt
     1141 cgtccggatt gaaggcgacc gcgctcgatg agcttgcggc gcccgcgagc accagcatcg
     1201 gaatgcttgg gtcgtctcgt cgcagcaacc ggtgggcctg cacgagatcg tggacgttct
     1261 tgcgcggctc gagagtgccg acgaacagca aatatcgctg cggaatcccg tgcgtttgca
     1321 gccatttatc ggacggcggg gtggcggccg cccatgcttc gtcgacgccc agcggggtca
     1381 cgacgatgcg gtcgcgattg gtgccataga cctcgacgag gtcgtctgcg gcggcgttgg
     1441 acggggtgat gatcacccgc gcgcgatcga gtgcgcgtgg caccaagtgc tggtaggcca
     1501 gcgacgtgct gtcgacggtc tcggggtgca agacgaagct cacgtcatgc acagtgacga
     1561 caccggcggc gcgccttgtg ggcggcagca cgaagttggt tccgtggaag acgtcgacgt
     1621 tcccgctgaa catctcgacc ggcgggaagt tgctgtggcc ccacgccgcc cggagcagtc
     1681 gggccggcgc gcgtcgcgaa tgcacgtcga ctccggcggc ctcgatctcg cgcaggtgct
     1741 gttgtccgcg ccaggtgaaa gccgtcagga ctacgtcgat atcggcctca ttggcaagcg
     1801 ccgccgagag ttgcgcgacg taatgtccga tgccggtacg ggtgccgagg aggggggtgg
     1861 cgtccagcgc gactcgcacc cgcgaatgct gtcacacggc cgcgtccact gctgtgagac
     1921 gggctggcgc gccgccgtga catacgatgg gacgccgagc aacgccgccg aacccccgaa
     1981 cgcacctatc ccgaaatgag cccacctgcc gtgacccaga tggcggagat gtggcgctat
     2041 cgcgggctca tcgggaactt cgcgagtcgc gaactgaaat cgaaatacaa gcacagcctg
     2101 ctcggctgga cgtggtcgct gatcaacccc gcagccactc tggccgtcta ctccattgtc
     2161 ttctcgacga tcttccgcgc gctgccgccg aaggcgcaca acggcatgac gacgtatgtc
     2221 atctacctgt tcatcgggtt ggtggcatgg aacttcttca gcaacgtggt gaacggcagc
     2281 atggcggccc tcatcggcgc tgggccattg ctcaagaaga tctactttcc gccctacgca
     2341 gcggtattgg gtaactccct gtctgcactc gtgcagacct tcatcgaggc cggtgtgctg
     2401 ctcgtggtgc tgctcgccgc aggcaatgcg tcgtggacgc tgctgctcgc cccggttgtg
     2461 cttgccctgc tgctcgtgtt cgcatcgggg atcggtctgc tcttcgcgct cttcaacgtg
     2521 ttctaccgtg atgtcagcta catcgtcacg gttatcctcc agctgctctt ttacgcaacg
     2581 cctgtcatct gggcgccttc gcagctgctc gggccgacgc acaaacatgg cgagttcgcg
     2641 gtgcacgtgc tctcgtacaa cccgatgttc cagtacgtcg aggcctttcg cgacgcactg
     2701 tgggagggtc acgtgcccag cttgtcgagg tgggcatgga tgctcggggg cgccgtcgtc
     2761 agcattacga tcgggttgat ggtgttcatc cggttctctc gcgatgtcag cgaggagttg
     2821 tgactcttct gcatcccgac agtcgtccac ctgctgttgt cgtcgatcac gtcaccaagg
     2881 agttcaagct ttacaaggat cggcgcacga gcttgaagga gcgcttcacc aagcggcgtc
     2941 cgagtcgcta cgacatgttc agggcgctcg acgacatcac gctcaacgtt cccgccggaa
     3001 ccacctatgg ccttatcggt tcgaacggtt cgggcaaatc aacgctgctg aagttgatgg
     3061 ccaacattca tcgtccgacg agtggcacgg ttactcacgc aggccgcatt tcggcgttgc
     3121 tcgaactcgg tgccggcttt catcctgagc tgtcaggccg cgacaacgtc tatctaaacg
     3181 gctctatcct cggcatgacc cgcaagcagg tcgatgccgc aatggacgcc atcatcgatt
     3241 tcagcggtat cgaggagttc atcgatactc cggtcaaggt gtattcgagc ggtatgtacg
     3301 tgcggctcgg attctcgatc gccgtcaatc tcgatcccga gattctcatc atcgacgaga
     3361 tcatcgccgt cggtgacgcg gagtttcagc gacgctgctt cgactacctc tacgagcttc
     3421 gcaagcgcgg cgtcacgatc atttttgtgt cacacagcct gccgctcgtg cagacgttgt
     3481 gcgaccgcgc ggcctggatc gagaagggcg tgttgagcgc cgagggtccg gccatgaagg
     3541 tcgtcgacga gtacatcgcg acggtcaaca aggcagagcg cgagcggttc gaagcagaag
     3601 ggcacggccg ggcggacggc gacacgcagc gtcgcggcga tggcgaggcg accgtcaccg
     3661 gtttgacgtt cctcgacgca tcaggggagc cgacgttggc agtcgttgcc ggcgaacgcg
     3721 tcacgttgcg gctttcgtac gaggccacca ctccggtcga cgatccggtc ttcggcctcg
     3781 gcttccagac cgagaacggg gtcctcgtca gcgggccgaa ctcgcaattc ggtggcgtgt
     3841 gcctgggtcg catctacggc aggggctaca tcgactacgt gctcgacccg ctccagttga
     3901 ccccggctac ctatctcgtc tcgaccgcga tcactgactc cacgcttttg cacgtctacg
     3961 actaccgtga cagagctttc accctcgccg tccagcctgg aaaatcgcct gaccgcaacg
     4021 gtctcgtgac cctcgacgga cattgggtgc tgccaacata aggttgtccc gggttattca
     4081 cgctaggacg gagttcgcgc agtgagggtc aatctcgggt gtggccaggc ctacatggag
     4141 ggctggacca acgtcgacgc cagccccgac gtcaaggctg acatccacct tgatgcggct
     4201 gagttcgtgc ggcagtacgg cgaccaggtc gacgaggtct acatgggcca cgtgatggaa
     4261 cacctgatgc cgggctacgc gtcgtccctc ttgcgattgc ttaacgagcg gctgcggccc
     4321 gggaccgtca tctccgtcgt cacccctgat atgcgggcga ttttcgaggc gtatctgtcc
     4381 ggtgagatcg acaacgacaa gttgaatgcg tcgttcattt actcctacgt gcagccctcg
     4441 catcacgtgt ggtgctacga cgaggccacg ttgctcgagt tgttccggcg cgcgggtctg
     4501 caaaatgccg aaccgatttc tgaccccacg atatatccgc cggtcttcca caaggtgggt
     4561 cctgagtcgc agtggcaatg cggcgtgcgc gccctggctg tcggccacga agcgccccca
     4621 ttcgaagtag aggtcaacga actcgacggc atcgatcgca cgtcgcatat cagcagcaac
     4681 gtcgaggcag taactcccga gcacgagttg ctgaaccgga tcgagaatct gcgcgcctcg
     4741 ctgatccgcg aatacgaacg ccgcaaggct cttgaggcca gactgacggt ttcgcctgaa
     4801 gagaccaccg caccggaagc gggcgtcaag cccgtcgagc cacctgtccc cgtctcgacc
     4861 gtcggcggcc gccgcgaggt cgcagctgca ccgactgagg tagcggatga gtctgcgccg
     4921 tcgctgttga gcccggccgg cgccaaagcc cgcgcgttcg acgtcgtcaa gcggacgctt
     4981 ccgcagggct cgaagtcgcg caacatcgtg cgcggtgggt tgctcgccta tcgcgaaagc
     5041 cgacgggtct cgcgcgacgt gcgggctctg ctcgccgaaa gcggcgtggt cgagccgcac
     5101 gagcccagct actacacgtg gtaccgacgc caccgtgcca caactgccga gcacaaggcg
     5161 cagcgcgatg cgtcggcggc cgcatctcaa ccgctgcacg ttctcgtcat cgtcttcacc
     5221 ggccccgagg agaacggcaa cgcgaccgcc acggtcgaga gcattcgcaa gcagacctgg
     5281 cagcactggg acgtcgaggt gtgcgccgac gcgggtcacg cgcccgacat caagggcgag
     5341 cacatcaggg tgcgcgaggg cgacacccga gtgactgcgg cccactacgc gatcgccgaa
     5401 tccgacgccg acttcgtcat cttgctcgac gccggagacc gcctggtcag ggaatgtctc
     5461 taccaggtgg ctcgcgcggc ctggcaggac ccgctcgtcg atctcgtgac gtgggacgac
     5521 gatttgctcg gtcgcgacgg acgtcgccac gatccccgct tccgaccgga gtggtcgccc
     5581 gaatcgatgg tcggcgccgg atacctcggg cagtcgttcg cgatccgccg tcgccgggcc
     5641 ctgttcgccg gcaacctgcg caacacctac ggcggcgcta cccgctggga tttgctgctg
     5701 cgctctgatc tcaatgccga gcgggtagtg cgcgtgccac gcgtgctcgc ccatgtgcgg
     5761 cagcgacgcc agggcatcaa ccccgacggc gtccgcgtcg tgcaggaagc tctagacagc
     5821 cgtggagtgc ccgccgtcgc cgagcttgca gaccagagcg tgcggttgcg ctggaagctg
     5881 gagaactggc cgcacgtcac gatcgtgatc ccgactcggc acaaccagcg gatgctctcg
     5941 aaggtgctgc ccggtctcgc cgcgactgac tacccgtcgt tcgacgtgcg cattgtcgac
     6001 aacggtggcc aaacggctga ccacgaggct tggtacgcgc agaacaccgc aggccttgat
     6061 ctgtcggtgc agtggtggac cgagaccccg ttcaactact cgcgagtcaa caacgctgct
     6121 gcggcgggcg cgcttggtga ggtgctggtc ttcctcaacg acgacatcga actgcccgac
     6181 tcgtcgtggt tgcgcgagat cgtcggctgg gccacgcaac ccgagatcgg tgtggcgggc
     6241 ctccagctca cgcgggctga cgggaagatt cagcacgccg gggtgatcct gggcatgggt
     6301 gggttcgccg accatgtatt cgagggcatg tcgccagggt ccgactcgct gcttggtccg
     6361 accgactggt accgcaacgt gcttgcggtg acgggggcat gtcttgcgat ccggcgagaa
     6421 accttcgacg ccgtaggcgg attcgatgag cgcttcatcc tgtgcggtag tgacgttgcg
     6481 ctcgggctcg acaccactca gctcggcctt cgcaatgtgt gctcgccttt tggcggcgtg
     6541 cgccatctcg aatcggcgac acgcggcagc cagataccga ccgaggattt ctttgccagc
     6601 tattggcgct acaacacctg gctgttcggc ggcgacccgt acttctctcc gagcgtatcg
     6661 ttgtcgagcc gcaagccgaa gctcgttggg ctgcaagaac ccacgccggc gcaacgcctt
     6721 tcgagtccac tcggccgcga tcttcaggta ttccgccagc gcagcgacgc agcagagtcg
     6781 acgatgctgg ccgatacctg ccgcgtcaac gatgtcgatg tcgctgccat caagaatctg
     6841 cacgcgagta acgcggcaca cctcgacgtc cgcactgtga actggtttat ccctgacatc
     6901 gactcgccgt tctacggtgg catcaacacg gcgttgcggc tggccgacta tttgcatcgc
     6961 aaccacggtg tgcagaacag gtttgtggtg tgggggagcg gacccgacta ctacgtgcgc
     7021 tcggcgatca ccgctgcctt cccgtcgctt gccgacagcg agatcagttt cttcgacggt
     7081 cccgacagcg cgagcctcga actcgtcccg ccggccgatg cttcgatcgc cacgctgtgg
     7141 ctcaccgcgt acgcagtcgc gcgggcccag aacacgaagc gaaaattcta cctggttcag
     7201 gatttcgagc cgatgttcta tccggcgagc acaccgtatg cactcgccga agagtcgtac
     7261 cggctcggtc tttacgggct ttgcaacacc gacaacatgc gccagatcta cgtcgacgac
     7321 tatcaaggca gggcgatgtc gttctcacct gctgtcgatc cgaccgtgtt ccacgcgaac
     7381 aaccggccat tccgcagcga cgactcgcct gcgaccgtct tcgtttacgc gcggccgggg
     7441 cattggcgta actgctggga actcgcgtca ctggcgctgc gcgaggtcaa ggagcggctc
     7501 ggcgaccagg tgcgcattgt gaccgcgggc tcgtgggcga ccgaccctga tgccgccgat
     7561 gcgatcaagc agctgggcct gctgccgtac aaggcgacag gtgagctcta ccggcattgc
     7621 gacgtcggcc tggcgctgac ggtgtcgaag catccgtcgt acctgccgct cgagctgatg
     7681 gcgtgtggcg ttcccgtggt ggcgttcgac aacccgtggg gctactggat cctcaaggac
     7741 ggcgagaact cgttgctcgc tcgtcgcacg gtgcctggca tcgtcgatgc gctcgagcgg
     7801 gcctgcctga accgcacgct gcgcaaagaa ctcgcggccc acgcagtggc tgatattgcg
     7861 gcccggcacg ggagttggga tgccgcattc cgcggaatct acgactactt gtgcgacccc
     7921 gaaggccact aaccgcagtg gtttccggca gcgcggtgcg tcggcgggtc ggcgtcgtca
     7981 cgacagagcc gttgaccgag cagctggccg gcccagcgat tcgcgcggtc gaactcggcc
     8041 gggtgctcgc caccgagcac gacgtcgaag tcgtctcgac cgcgttgtgc agcggcccgg
     8101 ggcccgaggg gaattggcgc tacgccgacg tagcagcact gcgtgaactc gccggccggt
     8161 gcgatgtcgt cgtgctcggt ggcgatgtcc tcgcagccaa tccgtggttg accgacgcag
     8221 ggccggcgat cgtcgtcgat ctgtacgacc cgttccatct cgagcaacta gagcaagctc
     8281 gcgatctcgg cgaggacgag cggcggcgcg tcgtgttcgg ctgcatcgac gtgctgaaca
     8341 cgcaagcagt gctcggcgac acgttcctgt gcgcatcgac tcggcagcgc gacatgtggc
     8401 ttggtcacct cgccgcctgc ggacgcgtga acccagcgac gtatgacgcc gacccgacac
     8461 ttcgcagctt gctgcggatc gtgccgttcg gggtcgccgc cgagcctccg attgccggtg
     8521 cccccgcact gcgcggcgtc gttgacggca tcggcgtcga cgacgagatc ttgttgtggg
     8581 gcggcggaat ctacaactgg ttcgatccca agacgttgat ccgcgccgtc gacgcgctgc
     8641 gaatctctcg accgaaactc cggttggtgt tcatgggggt ccagcaccct aatcccgtgg
     8701 tgaagacgat gcgggcagcg gccgaggcgt gggagctgtc gaacgaactc gagctgaccg
     8761 acgtccacgt cttcttctct gattcgttct cgtcgggttg gattccctac gcagaaaggg
     8821 gtcgttacct cgccgaggcg acgatcggcg tgagcacgca tcacgatcac gtcgagaccg
     8881 cgttctcgtt tcgcacccgg atcctcgact acttctgggc cgggctgccc gtggtcacga
     8941 cccgtggcga cgcgctcgcc gaggtcatcg agcgagcggg tgccggtcgc acagtcgagc
     9001 caggggacgt cgatgggctg gcggccgcga tcaacgattt gctggacgac gaggtggccc
     9061 gtagctctgc cgcagcggcc
//
LOCUS       DASWVF010000778         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_149398, whole genome shotgun sequence.
ACCESSION   DASWVF010000778 DASWVF010000000
VERSION     DASWVF010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149398"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGD55_13800"
     CDS             complement(<1..776)
                     /locus_tag="VGD55_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY0871468.1"
                     /translation="MRIAVTGSIATDHLMSFPGRFADQLMPDQLHNVSLSFLVDRLEI
                     RRGGVAANICFGMAAFGLRPILVGAVGADFDDYRSWLERHGVDTASVHVSYLHHTARF
                     VCTTDTDRNQIASFYAGAMSEARLIELAPVAARVGGLDLVVISPNDPDAMLRHTDECR
                     HRGYRFVADPSQQLTRMEGPQIRELIEGAEMLFTNEYEKAMTEAKTGWSDADVLARVG
                     VRVTTLGAFGAVIERAGEPPVKVAAAPERTLADPTGVGDG"
     gene            complement(937..1311)
                     /locus_tag="VGD55_13805"
     CDS             complement(937..1311)
                     /locus_tag="VGD55_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0871469.1"
                     /translation="MTVQDETTTLTEPTTGTTGVVLTAQAAAKVKALLEQEGRTDLAL
                     RVAVQPGGCSGLRYQLYFDDRSVDGDQVLDFGGVNVVVDKMSQPYLAGASVDFVDTIG
                     KQGFTIDNPNAGGSCACGDSFH"
     gene            complement(1385..2425)
                     /locus_tag="VGD55_13810"
     CDS             complement(1385..2425)
                     /locus_tag="VGD55_13810"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY0871470.1"
                     /translation="MSGASVLIAPDSFGGTMSAVQAAEAIASGWREAGALTLLPLSDG
                     GPGFVDVLHAALAGQLVDVEVSDPLGRLTPAQVLLVDATAYVESAQACGLHLLKPEER
                     DPSRTTSAGVAALLQAAVNAGADRIVVGLGGSGTNDAGAAMIEALDPDLLRGVELLVA
                     TDVDNPLLGPTGATAVFAPQKGADPDSLPFLEQRLAAFAAVIGPEFADLPGAGAAGGL
                     GFGLFSLGGHRVSGADLVLRLLDVGGHIAASALVITGEGSLDEQSLRGKLPARVAAAC
                     LDAGVPCVAIAGRVLLGRRQLAAAGFAEAHSIEGFAGSVEAAIAGGAAALRGLSAQVA
                     ANWSASSRRDWG"
     gene            complement(2422..3003)
                     /locus_tag="VGD55_13815"
     CDS             complement(2422..3003)
                     /locus_tag="VGD55_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871471.1"
                     /translation="MGFLDTVLGRTKPAKADLDDLFALPSAALTLEAGLDFKPTGLGS
                     VCFKAAEGAAFARTQDDVQQLLDASAAGSAPKVELSKDAFGYTWLLVRHDPTDVPGLV
                     TDLHAVNSGLQDAGFGPQLLCSLISFAHADGRRLALVYLYKRGTFYPFAPLGTSQRRD
                     NALEIQAQGTVVNDMKVEPDLTRWFPVWGAPGL"
     gene            complement(3004..>3312)
                     /locus_tag="VGD55_13820"
     CDS             complement(3004..>3312)
                     /locus_tag="VGD55_13820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0871472.1"
                     /translation="VYAISFLLIRALSRYRELSADRSGALLTGRPSALASALQKVTGA
                     MGQIPTRDLRSAESFNAFFFTPALAPGFSLSSLFSTHPPLDKRLAQLAEISRELGQGR
                     "
BASE COUNT          572 a         1101 c         1141 g          498 t
ORIGIN      
        1 aaaccatcgc cgaccccggt cggatctgcc agcgtgcgct cgggtgcggc cgccaccttc
       61 acaggcggtt cgccggcccg ttcgatgacc gcgccgaagg cgccgagggt cgttacgcgc
      121 actcccactc gcgccagcac atcagcgtcc gaccaaccgg tttttgcctc tgtcatagcc
      181 ttttcatact cgttcgtgaa caacatctcg gcgccctcga tcagctcgcg gatctgcggg
      241 ccttccatgc gggtcagctg ctgcgaaggg tcggcgacga agcggtatcc gcggtgacgg
      301 cattcgtcgg tgtgccgcag catcgcgtcg ggatcgttcg ggctgatgac caccagatca
      361 aggccgccga cccgcgccgc gaccggcgcg agctcgatca gcctggcttc gctcatcgcg
      421 ccggcataga aggacgcgat ctggttgcgg tcggtatcgg tcgtgcacac gaatcgcgcg
      481 gtgtgatgca agtaggagac gtgcacggac gcggtgtcga cgccgtggcg ctcgagccac
      541 gaccggtagt cgtcgaagtc ggcgccgacg gcccccacca ggatcggccg caacccgaac
      601 gccgccatgc cgaagcagat gttggcagcg acgccgccgc ggcgaatctc gaggcggtcg
      661 acgaggaacg acagcgagac gttgtgcagc tggtctggca tcaactggtc ggcgaaccgc
      721 ccggggaacg acatcaggtg atcggtcgcg atcgacccgg tgacggcgat ccgcatcaca
      781 ggaccggcag gtggcgcagg tggcatatgc ctaactcagc tccaagctcg cttcgtgacc
      841 gctcatacca accgctcata ccaagggcct cgccgtgagg cgaggccctt gtacgttaac
      901 gggtcaccgg caaaacgcca gccaccgcgg gtcgaactag tggaacgagt cgccacaggc
      961 gcacgagccg ccggcgttgg ggttgtcgat ggtgaagccc tgcttcccga tggtgtcgac
     1021 gaagtcgacc gaggcgccgg cgaggtacgg ctggctcatc ttgtcgacca cgacgttgac
     1081 gccgccgaag tcgagaacct ggtcgccgtc gaccgagcgg tcatcgaagt agagctggta
     1141 gcgcagaccc gaacagccac cgggctggac ggcgactcgg agcgcgaggt cggtgcgacc
     1201 ctcctgctcg agcagcgcct tcaccttcgc ggccgcctga gcggtcagaa ccacgccggt
     1261 cgtaccggtc gtgggttcgg tgagggtggt ggtctcgtct tgaaccgtca tggatgttct
     1321 ccctggagtt cgaacctgga tctgcgtccc atcttctcac ggccaacacc gcgatgcgcg
     1381 ctggtcatcc ccaatctcgg cgcgaactcg ccgaccagtt agccgcaacc tgggcggaca
     1441 gaccccgaag ggccgccgct cccccggcaa tcgccgcctc cacggagccg gcgaagccct
     1501 cgatcgagtg cgcctcggcg aatccagccg cggccagctg tcggcgtccg agcaagaccc
     1561 ggcctgcgat cgcgacgcac ggaacccccg catcgaggca cgcagcagcc acccgggcgg
     1621 ggagtttgcc ccgcagcgac tgctcatcga gcgagccctc gccggtaatg accagggcgg
     1681 acgccgcgat gtggccgccg acgtcgagca gccgcagcac caggtcggcg ccgctgacgc
     1741 ggtgaccccc gagtgagaaa agtccgaatc cgagcccgcc ggccgcgccg gcgccgggaa
     1801 ggtcggcgaa ctcaggtccg atgacggccg caaaggcggc cagccgctgc tcgagaaagg
     1861 gcagcgagtc ggggtcggcc cccttttgcg gtgcgaagac tgcggtcgcg ccggtcggcc
     1921 cgagcagcgg gttgtcgacg tcggtcgcca ccagcagctc gacccctctc agcaggtcag
     1981 gatccagcgc ctcgatcatg gcggcgccgg cgtcgttggt accggatccg ccgaggccga
     2041 ccacgatgcg gtcggctcca gcgttgaccg cggcctgcag cagcgccgcg acgccagcag
     2101 acgtcgtccg gcttgggtct cgttcttctg gcttgagcag gtgtagcccg caggcctggg
     2161 ccgattcgac gtaggcggtg gcgtcaacga gcaacacctg tgcaggggtc aagcggccga
     2221 gcggatcgga tacctcgacg tcgaccagct ggcctgcgag cgcggcgtgc agcacgtcga
     2281 cgaagcctgg gccgccgtcg gaaaggggca gcagcgtcaa cgcgcctgct tcgcgccacc
     2341 ctgaagcaat cgcttcggcc gcctgaacgg ccgacatcgt gccgccgaaa gagtcgggcg
     2401 cgatcagtac cgacgccccg ctcacaagcc gggcgcgccc cagacgggga accagcgggt
     2461 gaggtctggc tcgactttca tgtcattgac gacggtgccc tgggcttgga tctcaagggc
     2521 gttgtcgcgg cgctggctcg tacccagggg cgcgaacggg tagaacgtgc cgcgcttgta
     2581 gaggtagacc agcgcaagtc gtcgcccgtc ggcgtgcgcg aacgagatga gtgagcagag
     2641 caattgcggt ccgaatccgg cgtcctgcaa gccgctgttg accgcatgca gatctgtcac
     2701 gagacccggc acgtcggtcg ggtcgtggcg cacgagcaac caggtgtagc cgaacgcatc
     2761 tttgctgagc tcgactttcg gagcactgcc ggccgctgac gcgtcgagta gctgctgcac
     2821 gtcgtcttga gtgcgggcga aggcggcgcc ctcggcggct ttgaagcaaa ccgagccgag
     2881 cccggtcggc ttgaagtcga gcccggcctc gagtgtgagc gccgccgacg gcagcgcgaa
     2941 caggtcgtcg aggtcagcct tcgccggctt ggtgcggcca agcacggtgt ctaagaaccc
     3001 catttaccga ccctggccca gctcgcgtga gatctcggca agctgcgcga gccgcttgtc
     3061 gagcggcggg tgcgttgaga acagcgacga caacgagaag ccgggggcga gcgccggggt
     3121 gaagaagaac gcgttgaagc tctcggctga acgcaggtcg cgggtcggaa tctggcccat
     3181 cgcgccggtg accttctgaa gcgcgctcgc gagcgccgac gggcggccgg tgagtaacgc
     3241 gcccgagcgg tcggcagaca gctcgcggta acgcgatagc gccctgatga gcaagaagct
     3301 gattgcgtaa ac
//
LOCUS       DASWVF010000779         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_47451, whole genome shotgun sequence.
ACCESSION   DASWVF010000779 DASWVF010000000
VERSION     DASWVF010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47451"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGD55_13825"
     CDS             complement(<1..956)
                     /locus_tag="VGD55_13825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY0871473.1"
                     /translation="MSSPLVQLATTLVHGGSLPGASTPDPLAIHSPSIEYRALAPMLV
                     VFGAATVGVLLEAFLPRRQRAFAQTTLALLGLVAAFVVVLVNAGSDNLRKVVVEGAVA
                     MDGPALFIQGTILAVSIGGLLLIADRSFDADGDFVAQAADVPGSADERASVRAGFTHT
                     EVFPLAMFAVAGMLLFASADDLITMFVALEVLSLPLYLLVGMARRRRLLSQEAAVKYF
                     LLGAFSSAFFVYGLAMVYGFADTVQFSGIADALTSSTRGDSLVLLGVGLIGIALLFKL
                     SGAPFHWWTPDVYQGAPTSITAFMAAGTKIAAFGALLRVFFVA"
     gene            complement(953..>1661)
                     /locus_tag="VGD55_13830"
     CDS             complement(953..>1661)
                     /locus_tag="VGD55_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0871474.1"
                     /translation="TPVVLTLAVIGVIYGALQAIGQKDILRLLAYVSVSHFGLITLGI
                     FAFTSQGQTGATLYMVNHGFSTGALLLVVGFMIARRKSRGINDFGGMQKVTPILAGTF
                     LVACLSGLALPGLSTFVSEFLVLIGTFTRYKVAATFGTFGIILAALYMLILYQRTMTG
                     PVSPDVEGSHDLSLREKWVIAPVVAIIVVLGFYPRPILDVITPAVSSTLQQTGNTDPA
                     PVVPLSVAVGTTSGGSS"
BASE COUNT          381 a          509 c          548 g          223 t
ORIGIN      
        1 gccacgaaga agacgcgcag cagtgcgccg aacgcggcga tcttcgtccc agccgccatg
       61 aacgccgtga tcgacgtcgg tgcgccttgg tagacgtcgg gggtccacca atggaatggg
      121 gcgccggata gcttgaaaag caaggcaatt ccgatgagcc cgacacccag caagaccaac
      181 gagtcgcctc tggtcgatga cgtaagcgcg tcggcgatgc ccgagaactg aacagtgtcg
      241 gcgaagccgt acaccatcgc caggccgtag acgaagaacg ccgacgagaa cgcaccgagc
      301 aggaagtatt tgactgctgc ttcctgcgag agcaggcgac gccggcgggc catgccgacg
      361 aggaggtaca gcggcagcga caagacctca agcgcgacga acatcgtgat gagatcatca
      421 gccgaggcga acagcagcat gcccgcaact gcgaacatcg cgagcggaaa gacctcggtg
      481 tgggtgaagc cggccctgac actggcccgc tcgtcggccg accccggcac gtcggccgcc
      541 tgcgctacga aatcgccatc ggcgtcgaac gagcggtcgg cgatcagcag cagcccaccg
      601 atcgagaccg cgagaatcgt gccctggata aaaagcgccg ggccatccat cgcgacggca
      661 ccctcgacga cgacctttcg caggttgtcg ctgccggcgt tcacgaggac gacgacaaag
      721 gcagccacca ggccgagcaa tgcgagcgtg gtctgcgcaa atgcccgctg ccgtcgggga
      781 agaaaagcct cgagaaggac gccgacagtg gccgcaccga ataccaccag catcggtgcg
      841 agcgcgcggt actcgatgct cggtgaatgg atcgcgagcg gatcaggcgt cgaggcgccc
      901 ggcagcgacc cgccgtgcac cagcgtcgtc gcaagctgca cgaggggtga gctcacgagg
      961 accctccgga cgtggtgccg accgcaacgg acagcggcac gacgggcgcc ggatcagtat
     1021 tgccagtctg ctgcaaggtt gacgacaccg ccggcgtaat gacgtcgaga atcgggcgcg
     1081 ggtagaagcc gaggacgacg atgatggcaa ccaccggagc gatgacccac ttctcgcgca
     1141 gtgacaagtc atggctgccc tcgacgtccg gcgaaacagg tccggtcatc gtgcgttgat
     1201 agaggatgag catgtaaagc gcggcaagaa tgatgccgaa cgtgccgaaa gtcgccgcca
     1261 ctttgtagcg ggtgaacgtg ccaatcagca caaggaactc gctgacgaag gtgctcaagc
     1321 cgggcagggc gaggcccgac aggcaggcga ccaagaacgt gccggcgagg atcggcgtca
     1381 ccttttgcat gccgccgaag tcgttgatgc cccgcgactt gcggcgcgcg atcatgaagc
     1441 cgacgacgag cagtaacgcg cctgtcgaga aaccgtggtt gaccatgtag agcgtcgcac
     1501 cggtctggcc ttggctagtg aacgcgaaga ttcccagcgt gatcaagcca aagtgagaca
     1561 cggacacata ggccagtagc cgcaggatgt ctttctgtcc gatcgcctgc aacgcaccgt
     1621 agatgacgcc gatcaccgca agggtcaaaa ccacgggggt g
//
LOCUS       DASWVF010000780         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_22326, whole genome shotgun sequence.
ACCESSION   DASWVF010000780 DASWVF010000000
VERSION     DASWVF010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22326"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VGD55_13835"
     CDS             complement(<1..555)
                     /locus_tag="VGD55_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HEY0871475.1"
                     /translation="MPSASADRLGPPSRPSRSLLLLDTPSLYFRAFYGVPESVTAPDG
                     TPVNAVRGLLDFIGFLVERYHPTHLVACMDADWRPAFRVALLPSYKAHRVAPAGGETV
                     PDALTPQVAVIEQVLDAIGLCRLGVAGFEADDVIATLAVRAADPVDIVTGDRDLFQLV
                     DDAKPVRIVYIARGVGKAELVDEAA"
     gene            680..1825
                     /locus_tag="VGD55_13840"
     CDS             680..1825
                     /locus_tag="VGD55_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0871476.1"
                     /translation="MKVDRELPTTQAGELLDLVSEVARDALAPRAAAAERDAAFPRDV
                     FTMLGRMSLLGLPYAEEHGGSGQPYTVYLQVVEELSRAWLTVGLGVSVHTLACFPLAR
                     FGTEAQRAQWLPGMLGGDQLGAYCLSEPDSGSDAGALRTRAENAADGYRLSGTKAWIT
                     HAGHADFYTVMARTSDNRSRGISCFLLPSDAEGIRAGVPEKKMGLRGSPTGQLHLDNV
                     AAGSERLIGNEGQGFTIALAALDTGRLGIAACSVGLAQAALDVALDYAGERRQFGQPI
                     GDFEGLGFMLADMATGVAAGRALYLAAARRADAGERFTMQAAMAKLFCTDMAMRVTTD
                     AVQVLGGYGYVEDYPVERYLREAKALQIVEGTNEVQRLVISRQLRGS"
BASE COUNT          326 a          622 c          657 g          268 t
ORIGIN      
        1 ggcggcctcg tcgacgagct ccgccttgcc gaccccgcgg gcgatgtaga cgatgcgcac
       61 cggcttcgcg tcgtcgacga gctggaacag atcgcgatcg ccggtcacga tgtcgaccgg
      121 gtcggccgcg cgcacagcca acgtggcgat gacgtcgtct gcttcgaagc cggcgacgcc
      181 gagccggcac aggccgatcg cgtcgagaac ctgctcgatc acggcgacct gcggagtgag
      241 cgcatcgggg acggtctcac cacccgcagg tgcgacccga tgcgccttgt acgacggcag
      301 cagggcgacc cggaacgctg gccgccaatc ggcgtccatg caggcgacga gatgagtcgg
      361 gtggtaacgc tcgaccagaa acccgatgaa atcgagcaat ccgcggaccg cgttgaccgg
      421 cgtcccgtct ggcgcagtga cgctttcagg cacgccgtag aaggcgcgaa agtacagcga
      481 cggcgtgtcg agaagaagca ggctgcgcga cgggcgagat ggcggaccga ggcgatctgc
      541 ggacgctgac ggcatgaccc cgagcctggc atggcgaacg ttggcgcgca cgcgagggcg
      601 ccgacgacgc acgaggtgcg gtctagattg aggccatgac acgcccgcca ggaggatcgc
      661 gcagtcactc gggcccgcga tgaaggtcga tcgggagctg ccgaccacgc aggccggcga
      721 actactcgac ctcgtcagcg aggttgcccg cgacgcgctg gcgccccgcg cagccgccgc
      781 ggaacgcgac gcggcgttcc cgcgggacgt cttcacgatg ctcgggcgca tgagcctgct
      841 cggtctgccg tatgccgaag agcacggggg atcagggcag ccctacacgg tctacctcca
      901 ggtcgtcgaa gaactcagcc gcgcctggct cactgtcggg ctcggggtca gcgtgcacac
      961 ccttgcgtgc ttcccgctgg cccgtttcgg caccgaagct caacgcgccc agtggctgcc
     1021 cgggatgctc ggaggtgatc agctcggcgc ctactgcctc tcagaaccag attcagggtc
     1081 ggacgccggt gcgctgcgca cgcgggccga aaacgcggct gacgggtacc ggttgtcggg
     1141 caccaaggcg tggataaccc acgccgggca cgcggatttc tacaccgtca tggcgcgcac
     1201 gtccgacaac cgatcgcgcg gcatcagctg cttcctgctg ccctccgacg ccgaaggcat
     1261 cagggccggc gtgcccgaga agaagatggg ccttcgtggt tcgccgacgg gccagctgca
     1321 tctcgacaac gtcgccgccg gatccgagcg gctgatcggc aacgaaggcc agggcttcac
     1381 gatcgcacta gccgcactcg acaccggccg gctcggcatc gccgcgtgct cggtgggcct
     1441 tgcccaagcg gcacttgacg tcgccctcga ctacgcgggg gagcggcgcc agttcgggca
     1501 gccgatcggt gacttcgagg ggcttggatt catgcttgcc gacatggcga ccggcgtggc
     1561 ggccggtcgg gcgctctacc tcgccgccgc ccggcgggct gatgcggggg agcgcttcac
     1621 catgcaggcc gcgatggcga agctattttg caccgatatg gcgatgcggg tcaccaccga
     1681 tgccgtccaa gtgctcgggg gatacggcta cgtcgaggac tatccggtcg aacggtacct
     1741 ccgggaggca aaagcgcttc agatcgtcga gggcacgaac gaggtgcaac ggctggtgat
     1801 cagccgacaa ctgcgcggat catgatgcga ccgacgagcc ggtcatggtc gccaaaggtc
     1861 gccaaaggtc gcc
//
LOCUS       DASWVF010000781         6722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_174578, whole genome shotgun sequence.
ACCESSION   DASWVF010000781 DASWVF010000000
VERSION     DASWVF010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174578"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGD55_13845"
     CDS             <1..552
                     /locus_tag="VGD55_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0871477.1"
                     /translation="LVPFMTLCQETCPLTTTNLVTMDKSVLDARQQNNVQIVEITIDP
                     ERDTVTQLDAYRGVAQLMQPNWSLLTAAPADLSRLWKLLGVDVERTAPDPGAVDWLGG
                     APLTYDLTHTDEVFFFDKSGHERFLIDGAPNTKGVLPSPVLDKFLNEEGQTNLMNPDA
                     STWTADDATASLAWVLGKHLKSP"
     gene            complement(581..1132)
                     /locus_tag="VGD55_13850"
     CDS             complement(581..1132)
                     /locus_tag="VGD55_13850"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0871478.1"
                     /translation="MSAKDELRREVLASRAGRPPSERTAVAAALAQVFSGAFGDRLSP
                     EFVVAVYISISGEPETGPLLEALGPARVIVPVLLPDRDLDWVVAGDPGATLGVEAIAS
                     ATVVLVPAVAVDRNGHRLGRGGGSYDRALTRVPDGRPVLAVVHDEEVLEALPVEPHDV
                     AVGGALTPSGVLWFGGFSPAGHG"
     gene            1316..3442
                     /locus_tag="VGD55_13855"
     CDS             1316..3442
                     /locus_tag="VGD55_13855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0871479.1"
                     /translation="MALRTRLSLAFICTVLVPVLAGAIVVLIAVPRVLHSQIANRLRT
                     AGVGVTNVLSAKCTEAAQAAQLLGVEVATLGPVSAVNRMVTSNAVGYAVVAGPGDTIV
                     ASAGSLPGAPGTKTPSPALLNSCAPGFENGSFAISSSASLAIAGSPSLTQVAVAWPVG
                     ATTAGSLSSGIVGRPAVTLVTHGLVVSSTLKAAAAHQQIRASASASAETAFDVGGRIL
                     AVVPASPGEPYDVIVSEPAPNVTNLTLVLIGVVLVAGLIALLMGSLLARLISRPVVEL
                     SDAAGRAAGGDLDITIPVRSKDEVGQLATAFNHMTSELQTYIRQLEHSRDELRQNLDR
                     LGATLTHTHDLGGILAVVLDTAIGSVQATGGAIMFLDADGELTVRVRRGQAAEAVDID
                     SRVPRGKGITGHVAESGEPVRGIVGEGPGLRPVPSEPKATTVIAVPLRQSGRIVGVLN
                     LYDKDPGYGTDLDAAETINPREFTAADLETILAFATQAGVAIDNVLLHQEAQRLSLTD
                     PLTGLWNYRYLTIGLGHEIERASRFSRPLALLMLDLDRFKAINDQYGHQVGDAVLIEL
                     AGRMRAEVREVDTVARYGGEEFVIVLPETDPVGAARLATRLGTVIRSTPFCVDLAANG
                     HSVEIGKGLPVSASIGVAVFPEHGASPTRLLRSADDALYAAKDAGRDGWRFAKPDVEG
                     EGDDQGRKVLVVPDVEAPARPRTTDG"
     gene            3464..4369
                     /gene="galU"
                     /locus_tag="VGD55_13860"
     CDS             3464..4369
                     /gene="galU"
                     /locus_tag="VGD55_13860"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0871480.1"
                     /translation="MADHKVTKAVIPAAGLGTRFLPATKATPKEMLPVVDKPAIQYVV
                     EEAVAAGLDDILMITGRSKRALEDHFDRNYELEDTLRAKGDADRLSLVQEATDLATVH
                     YVRQGEPKGLGHAVLVAAQHVGHEPFAVLLGDDLIDVRDPLLARMIEVQERLGGSVIA
                     LIEVDPAQIHLYGCAAVEATGEDDVVRVTDLIEKPKTNAPSNLAIIGRYVIAPEVFDV
                     LRETPPGAGGEIQLTDALRVLAGRDDRGGPVHGVLFRGRRYDTGDRAEYLRAVITLAV
                     GRADLGPELLAWLRIFVKQQDELQA"
     gene            4366..5589
                     /gene="glp"
                     /locus_tag="VGD55_13865"
     CDS             4366..5589
                     /gene="glp"
                     /locus_tag="VGD55_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY0871481.1"
                     /translation="MRTVDEHLTAVLGTLGPLPPLDVNLLDAHGCVLAEDVVATRPLP
                     GFDNSSMDGYAVRSVDVASATPETPVVLPVIGDIAAGSGGVYSVNNGLSVRIMTGAPI
                     PAGADCVIPVEWTDGGVARVRIDHAPSPGSYIRRTGEDVTANETVLTKGTALGATQIG
                     LLAAIGRARVMVRPRPRVVIFSTGNELVDVGVEPGPAQINESNSYTLTSAAREAGAIA
                     YRVGIVPDDPTKLMAIIEDQLVRADLVVTTGGVSAGAYDTVKAVLSRLGTVEFVKVAM
                     QPGMPQGFGTVGPDSTPIFTLPGNPVSSYVSFEVFVRPAIRRMLGVLPLYRPLVRATL
                     ASPITKPEGKRSYVRARIEVTEGVYVVTPVGGMGSHLVADLAMANAFVVAPDSATEIP
                     AGSTVSVMMLERRQS"
     gene            5586..6065
                     /gene="moaC"
                     /locus_tag="VGD55_13870"
     CDS             5586..6065
                     /gene="moaC"
                     /locus_tag="VGD55_13870"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0871482.1"
                     /translation="MSSDGFSHIDESGAARMVDVSAKDITVRTATASGRVVVSAAVIR
                     LLRGGGVPKGDALAVARLAGIQGAKRTPELIPLAHPIALHGVTVELVVDDDAVRISAT
                     ARTADRTGVEMEALTAVAVAGLALIDMVKGVDQAAVITDIRVEQKTGGSRGRWDRDG"
     gene            6071..6577
                     /locus_tag="VGD55_13875"
     CDS             6071..6577
                     /locus_tag="VGD55_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY0871483.1"
                     /translation="MTRALVITVSDRASAGMYADKGGPLIVAALAELGFEVDGPIVIP
                     DGSPVEVALRSALDAVYDIVITTGGTGLGPRDQTPQMTARILDYEVPGIPEAIRAAAH
                     LGVPTAVLGRGLAGVAGRTLIVNLPGSTGGVRDGMAVLTPILQHAVEQLAGGDHAGTS
                     EIGGDDAS"
BASE COUNT         1128 a         2301 c         2205 g         1088 t
ORIGIN      
        1 ctggtgccct tcatgactct gtgtcaggaa acctgcccgc tgacaacgac caacttggta
       61 accatggaca agtcggtgct cgatgcgcgc cagcagaaca acgttcagat cgtcgagatc
      121 accatcgacc ccgagcgcga cacagtcaca cagctcgacg catatcgcgg cgtcgcgcaa
      181 ctcatgcagc cgaactggtc gctgcttacc gctgcacccg ctgatctttc gcggctgtgg
      241 aagttactcg gcgtcgatgt ggaaaggacc gctcccgatc cgggtgccgt cgactggctc
      301 ggcggcgcac cgctcacgta cgacctcacg cacaccgatg aggtcttctt cttcgacaag
      361 agcgggcacg agcggttctt gatcgacggc gcgccgaaca cgaagggggt gctgccgtcg
      421 ccggtgctcg acaagttcct caacgaggaa ggccagacga acctgatgaa tcccgacgcc
      481 tcgacgtgga cggccgacga tgcgaccgcg tccctcgcat gggtgctcgg caagcacctc
      541 aagtcgccct agcccgtcgc cgtcctacgt tcctcgcgcg tcacccgtgc ccggcgggtg
      601 aaaatccgcc gaaccacagc actccgctcg gcgtaagcgc gccaccgacc gccacgtcgt
      661 gcggctcgac cggaagagct tcgagaacct cttcatcgtg gacgacggcc agcaccggcc
      721 gcccgtctgg tacccgggtc aacgcgcggt cgtacgaccc gcccccgcgt ccgagccggt
      781 ggccgttgcg gtcgacagcc accgccggga ccaagaccac tgtggcgctg gcaatcgcct
      841 cgacgccaag cgtggcaccg gggtcgccgg ccacgaccca atcgaggtcg cgatcgggga
      901 gcagcaccgg cacgatcacc cgagccgggc cgagagcctc caggagtggc cctgtttcgg
      961 gctcgccgct gatcgagatg tagacggcga cgacgaactc agggctcaag cggtcgccga
     1021 atgcgcccga gaacacctgg gcgagagcgg cagcgaccgc cgtccgctca gagggagggc
     1081 gcccagcacg gctggcgagc acctcgcggc gcagctcgtc tttggcactc acgacggtct
     1141 cccactcggt gtcctatttg gccctttatg cggatgcaca gtaacgactt atcccccggc
     1201 cggcgtcttc ttagcggcca acgaccatgc gagcatcgca tggggtagtt gcgccgcatc
     1261 gctcccgatt cgacggtcat ctgaccgaca cgcgacgaac accggaggtg ccgacatggc
     1321 gctgcgcacc cggctttcgc tcgccttcat ctgcacggtg ctcgtcccgg ttctggccgg
     1381 ggcgatcgtg gttctgatcg cggtgccccg ggtcttgcac agccagatcg cgaaccggct
     1441 gcgcacggcc ggcgtcggag tcacgaacgt gctctccgct aaatgcaccg aagcagctca
     1501 ggcggcccag ttgctcgggg tcgaggtcgc caccctcgga cctgtctcag cggtcaaccg
     1561 catggtcacc agcaacgcgg tcggctacgc cgtcgtcgcc ggcccgggcg acacgatcgt
     1621 cgcaagcgcg ggatcgctgc ccggtgcgcc cgggacgaaa acgccgtcgc cggccttgct
     1681 gaacagttgc gcgccaggct ttgagaacgg cagcttcgca atctcgagca gcgcgagctt
     1741 ggcgatcgca ggcagcccgt cgctgacgca ggttgccgtt gcgtggccgg tcggtgcgac
     1801 caccgccggc agcctcagct ccggcatcgt cggccgaccg gcggtcacgc tcgtgactca
     1861 tggcttggtc gtctcgagca cgttgaaggc ggctgcggca catcagcaga tccgggcatc
     1921 ggcatcggcg tcggctgaga ctgcattcga tgtcggcggc cgcattctcg cggtggttcc
     1981 agcctcgccc ggtgagcctt acgacgtcat cgttagcgag cccgcgccca acgttacgaa
     2041 cctgacgctc gtgctgatcg gcgtcgtctt ggtcgcaggc cttatcgcac tcttgatggg
     2101 aagcctgctc gcgcggttga tcagcaggcc cgttgtcgaa ctatcggacg ccgcggggcg
     2161 ggcggccggt ggcgacctcg acatcacgat ccccgtgcgg tcgaaggacg aagtcggcca
     2221 gctcgcgacc gccttcaacc acatgacgtc tgagctccag acctacatcc ggcaactcga
     2281 acacagtcgc gacgaactgc ggcaaaacct cgaccgcctc ggcgcaactc ttacgcacac
     2341 gcacgacctc ggcgggatcc tggcagtcgt tctcgacacc gcgatcggca gcgtgcaggc
     2401 cactggcggt gccatcatgt tcctcgacgc cgacggcgag ttgacggttc gagtaaggcg
     2461 cgggcaagca gccgaggctg tcgacatcga ttcgcgggtg ccgcgcggca aaggcatcac
     2521 gggtcacgtt gccgaaagcg gcgaaccggt gcgggggatc gtcggtgagg gccccggtct
     2581 gcgcccggtg ccgagtgaac cgaaagccac cacggtcatc gccgtgccct tgcggcaatc
     2641 gggtcgcatc gtcggcgtgc tgaacctcta cgacaaggac cccggctacg gcaccgacct
     2701 tgatgcggcc gaaaccatca acccgcgtga gttcaccgcc gccgacctcg agacgatcct
     2761 cgcgttcgcg acgcaggccg gtgttgcgat cgacaacgtg ctgctgcacc aagaggccca
     2821 gcggttgagc ctcaccgacc cgctcaccgg gctgtggaac taccgctatc tcacgatcgg
     2881 gctcggccac gagatcgagc gcgccagccg attcagccgg ccgcttgcct tgctgatgct
     2941 cgacctggac cgcttcaagg cgatcaacga ccagtacggg caccaagtcg gagacgccgt
     3001 gctgatcgaa ctcgcgggac ggatgcgagc cgaggtccgc gaggtcgaca ccgtcgcgcg
     3061 ctacggcggt gaggagttcg tgatcgtgct gcccgagacc gatccggtcg gcgcggcccg
     3121 gctcgccacc cggctcggca ccgtcatcag atcgacgccg ttctgcgtcg acctcgcagc
     3181 caacgggcac agcgtcgaga tcgggaaggg cctgccggtc tccgcctcga tcggagtggc
     3241 cgtgttcccc gagcacggcg cctcgccgac tcgcctcctg cgcagtgccg acgacgccct
     3301 ctacgccgcg aaggacgccg gccgcgacgg gtggcgcttc gccaagcccg acgtcgaagg
     3361 tgagggcgac gatcaggggc gcaaggttct cgtcgtcccc gatgtcgaag ctccagcgcg
     3421 gccccggaca accgacggct gagtcgaacg ctagggtcgc tgcatggcgg atcataaggt
     3481 cacgaaggcc gtcatacctg ctgctggtct cggcaccagg ttcttgccgg cgacaaaggc
     3541 gacgccgaag gaaatgcttc cggtcgtcga caagcccgcg atccagtacg tcgtcgaaga
     3601 ggcggtcgct gccgggctcg acgacatctt gatgattacc ggccgcagca agcgcgcact
     3661 cgaggaccat ttcgaccgca actacgaact cgaagacacg ctgcgcgcca agggggacgc
     3721 cgatcggttg tcgctcgtgc aagaggcgac cgatctcgcg actgtgcact acgtgcgcca
     3781 gggggagccg aagggtcttg gtcacgcggt gctggttgca gcacaacacg tcggacacga
     3841 gccgttcgcc gtcctgctcg gtgacgacct catcgacgtg cgcgaccccc ttctcgcgcg
     3901 catgatcgag gtgcaggaac gtctcggcgg cagcgtcatc gcgttgatcg aggtcgaccc
     3961 ggcacagatt cacctctacg gttgcgcagc cgtcgaagcg accggcgaag acgacgtcgt
     4021 gagggtcacc gatctcatcg agaagccgaa gaccaacgcg ccaagcaacc tcgcgatcat
     4081 cggccgttac gtcatcgcac cggaggtctt cgacgtcctg cgcgagaccc cgccgggcgc
     4141 cggtggcgag atccagctca ccgatgcgct gcgcgtgcta gcaggccgcg acgaccgcgg
     4201 cggacccgtg cacggggtgc tcttccgcgg tcggcgctac gacaccggcg accgtgccga
     4261 gtacttgcgc gccgtcatca cgctcgcggt cggtcgtgcc gacctcggcc ccgagttgct
     4321 tgcctggctg aggattttcg tcaagcaaca ggacgaactc caggcatgag gaccgtcgac
     4381 gaacacctga ctgcggtgct cggaacgctc ggtccactgc cgccgctcga cgtcaacctg
     4441 ctcgacgctc acggctgcgt gctcgccgaa gacgtggtgg cgacgcggcc gctgccgggg
     4501 ttcgacaact cgtcgatgga cggctatgcc gttcgatcgg tcgatgtcgc ttcggcgacg
     4561 cccgaaacgc ccgtggtgct gccggtcatc ggcgacattg ccgcaggcag tggcggtgta
     4621 tattcggtca acaacgggct gagcgttcgc atcatgaccg gcgctccgat tccggccggg
     4681 gccgactgcg tcattcccgt cgagtggacc gacggtggcg ttgcgcgtgt acgcatcgac
     4741 cacgcgccat cccccggctc gtacatccgc cgcaccggcg aagacgtcac ggccaacgag
     4801 acggtattga cgaaaggcac cgcacttggt gccacgcaga ttggcctgct cgctgccatc
     4861 ggtcgcgccc gggtgatggt gcggccgcgg ccacgagtgg tgattttctc gacaggcaac
     4921 gagcttgtcg acgtcggcgt cgagccaggc ccggcccaga tcaacgaatc gaacagctac
     4981 accctcacct ctgccgcgcg tgaagctggc gcgatcgcgt atcgggtcgg catcgtgccc
     5041 gacgacccga ccaagctcat ggccatcatc gaggaccagc tcgtgcgcgc cgacctggtc
     5101 gtcacgaccg gcggagttag tgccggcgcg tacgacacgg tgaaggcggt gctgtcgagg
     5161 ctcggcaccg tcgagttcgt caaagtcgcg atgcagcccg gcatgccgca gggcttcgga
     5221 accgtagggc ccgactcgac accgatcttc acgctgccag gcaacccggt gagctcgtac
     5281 gtgtcgttcg aggttttcgt gcggccagcg atccggcgca tgctcggcgt cctgccgctc
     5341 tatcgtccgc tggttcgtgc aacactcgcg agcccgatca ccaagccaga aggcaagagg
     5401 tcgtacgtgc gcgcacgcat cgaggtcacc gagggcgtct acgtcgtcac cccagtcggt
     5461 ggcatgggat cccacttggt tgccgacctc gccatggcga acgcgttcgt cgtcgcaccc
     5521 gacagtgcca ccgagatccc ggcgggctcg acggtttcgg tcatgatgct cgagcggcgg
     5581 cagtcatgag ctccgacggt ttctctcata tcgacgagag cggcgccgct cgcatggtcg
     5641 acgtctcagc caaagacatc acagtccgca ccgcgactgc ttcaggtcgc gtcgtcgttt
     5701 ccgccgcggt gatccgcctg ctgcgtggcg gcggcgtgcc gaagggtgac gcactcgcgg
     5761 ttgcgaggct ggccggaatc cagggcgcga agcgcacacc ggaattgatc ccgctcgcgc
     5821 acccgatcgc gttgcacggc gtcaccgtcg aactcgtcgt cgacgacgat gccgttcgga
     5881 tctcggccac cgcgcgcact gccgatcgca ccggcgtcga gatggaagcg ctcacggccg
     5941 tcgcagtcgc cgggcttgcg ctcatcgaca tggtcaaggg cgtcgatcag gcagcggtca
     6001 tcaccgacat ccgggtcgag cagaagaccg gcggctcgcg tggccgctgg gatcgcgacg
     6061 gctgacgctg atgacgcgcg cgctcgtcat cacggtctcc gaccgcgcgt cggcaggcat
     6121 gtacgccgac aagggcggcc cgctgatcgt cgcggcgctg gccgagttgg gcttcgaggt
     6181 ggacgggccg atcgtgattc ccgacggctc gccggtcgag gtcgcacttc ggtcggcgct
     6241 cgacgcggtc tacgacatcg tcatcacgac gggtggcacc gggttgggcc cgcgcgacca
     6301 gacgccgcag atgacggctc ggatactcga ctacgaagtt ccgggaattc cggaagcgat
     6361 acgggcggcg gcacacctcg gcgtcccgac tgctgttctt ggtcgaggcc ttgccggtgt
     6421 cgccggacgg acgttgatcg tcaacctgcc aggatcgact ggtggcgtgc gcgacgggat
     6481 ggcggttttg actccgatcc ttcaacatgc tgtcgagcaa ctcgcggggg gcgaccatgc
     6541 cggcactagc gagatcggag gagacgacgc ttcgtgagcg cgggctggcc ggtctatctc
     6601 gagcacggcc cgatcaagct gcggccgtta cggttgcgcg acgcctccga ctggcggctg
     6661 acccgcatgc tcaatcgcga ctggctcgag ccctgggaag ccacgacgcc cggagccgcg
     6721 cc
//
LOCUS       DASWVF010000782         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_22356, whole genome shotgun sequence.
ACCESSION   DASWVF010000782 DASWVF010000000
VERSION     DASWVF010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22356"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1085)
                     /locus_tag="VGD55_13880"
     CDS             complement(84..1085)
                     /locus_tag="VGD55_13880"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY0871484.1"
                     /translation="MTLTVRTATPAAKVASTVDMTTTLGKFALPNPVLTASGCAAAGQ
                     ELAQFFDVAALGGIVTKSIMLNVRSGRATPRMAETPSGMVNSIGLQGPGIETFLDRDL
                     PWLIDQGARAVVSIAGGTVDEYAKLAMRLRDAGGVSMVEVNISCPNVEERGQVFACDA
                     RAAAEVVQAVRRATRSEIPVFAKLSPDVTDIVSIASACVTAGADGLSMTNTLLGMVID
                     TDTLRPVLAGVTGGLSGPAIRPVALRCVWQVHAAMPEVPILGMGGIRTGLDALQFILA
                     GARAVSVGTEIFHDPTAPVRVLAELEEALAQRGIKRLVDAVGLAHGTRVSAVTGTTG"
     gene            complement(1082..>1758)
                     /locus_tag="VGD55_13885"
     CDS             complement(1082..>1758)
                     /locus_tag="VGD55_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY0871485.1"
                     /translation="RGTAWLASRQAHERIDIVGPLGRPFSLPREPATCVLVGGGYGTA
                     PLFPLADALRARGCRVDFVMGAAREERLFGALDAKRISATLTVTTDDGSVGHHGRVSD
                     VLPDVLASSKADVVYACGPMGMLRAVSQVAAAGGLPCQIAVEELMACGVGVCMTCVLP
                     VIGDDGITRMVRSCVDGPVFHGDRVRFDDVGTIPADVHGAPVVIAAKTPARTSSPRKA
                     RAEVTR"
BASE COUNT          317 a          602 c          576 g          263 t
ORIGIN      
        1 gtggggttca acagcacgcg cccatgcgct tgcagtgtcg aggtcgcgtg catcgagcgc
       61 gaccgcgatg ggcgcggtcc cggtcatcct gtcgtaccgg tgactgcgct cacacgtgta
      121 ccgtgcgcca gccccacggc gtcgaccaat cgcttgatac cccgctgcgc caaggcctct
      181 tcgagttcgg caaggacgcg caccggcgcc gtcgggtcgt ggaagatctc ggtgccgaca
      241 gacaccgcac gcgcgccggc aaggatgaac tgcaacgcgt cgagacccgt gcggataccg
      301 cccataccga ggatcggtac ttcgggcatc gccgcgtgta cctgccaaac acaacgaagg
      361 gcgacggggc gaatcgccgg gccagacaat ccgccggtca cgccggcaag tactgggcgc
      421 agcgtgtcgg tgtcgatgac cattccgagc aaggtattcg tcatcgagag tccgtcggcc
      481 ccggcagtta cacacgcact ggcgatcgag acgatgtccg tgacatctgg tgacagcttg
      541 gcgaacaccg ggatctctga gcgcgtcgca cgacgcaccg cctgtaccac ctcggcggcc
      601 gccctggcgt cgcaggcaaa cacctggccg cgttcttcga cgttggggca ggagatgttg
      661 acctcgacca tcgaaacgcc gccagcgtcg cgcaggcgca tggccaactt cgcatattcg
      721 tcgacggtgc cgccggcgat cgacacgacc gcgcgcgcac cctgatcgat caaccaaggc
      781 aggtcgcgat cgaggaacgt ctcgatccct gggccctgca gcccgatcga gttcaccatt
      841 ccgcttgggg tctcggccat ccgcggagtg gcccgacccg accgcacatt cagcatgatg
      901 gacttggtga cgatgccccc gagcgcggcg acgtcgaaga attgagcaag ctcctggccc
      961 gccgcggcgc aaccggaggc cgtcaggact ggattcggta gtgcgaactt accgagcgtg
     1021 gttgtcatgt cgaccgtgct cgcgaccttt gcggccggcg ttgcggtgcg aacagtcagg
     1081 gtcatcgagt cacctctgcc cgggccttgc gcgggctcga agtgcgagcc ggggttttcg
     1141 cggcgatcac cacgggcgca ccgtgcacat cggccggaat ggtgccgaca tcgtcgaaac
     1201 gcacccggtc accatggaag accggcccgt cgacgcacga acgaaccatg cgggtgattc
     1261 cgtcgtcgcc gatcaccggt aggacgcacg tcatacagac cccgacgccg caggccatca
     1321 gttcctcgac ggcgatctga cagggcaggc cgcccgctgc agccacctgg ctgacggcgc
     1381 gcagcatgcc catcgggccg catgcataga ccacgtcggc cttgctcgaa gcaagaacat
     1441 caggcaggac gtcggagact cggccgtggt ggcccaccga tccgtcgtcc gtcgtgacgg
     1501 tcagcgtcgc ggagatgcgt ttcgcatcga gtgcgccgaa caaccgctcc tcgcgggcag
     1561 cgcccatcac gaagtcgacc cgacagcctc gcgcccgcaa cgcgtcagcc agcggaaaga
     1621 gcggtgcggt gccgtagccg ccgccgacga gcacgcaggt cgccggctcg cggggcaagg
     1681 agaacggacg gccaaggggg ccaacgatgt cgatccgttc gtgcgcttgc cggctggcca
     1741 accacgcggt gccacggc
//
LOCUS       DASWVF010000783         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_47539, whole genome shotgun sequence.
ACCESSION   DASWVF010000783 DASWVF010000000
VERSION     DASWVF010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47539"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VGD55_13890"
     CDS             <1..1149
                     /locus_tag="VGD55_13890"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEY0871486.1"
                     /translation="GPPASGPVALAATGPVTDSLSAVQFTRAPVNGEQQVSTAQCESP
                     TTDAWFAGFSSNVGAHATLLLSNVDSVPATVDVSIWGAQGGEPNVRRGITVDALTQVE
                     VPLDQIEPGLPVGIAHVVATAGRVVPALRGDAANGSIPLGVDWLPPTAAPATTLTVPG
                     ILGDIGSRRLIVGNPGTVDATFALTVVTADGAFMPTEFTSLTVDAGEVSDVSLDPVLQ
                     GDAAAIVVTSSQPVVAGGISALPLDGAGGSDFAFTGAAVPLAGPTVVAGGEVGAGRQT
                     QLVMSAPGDDASVRVVILPSAVDAPEVSNDMTVTGGTTTVLDLAVLTTDPAPAVVVIP
                     EGGGPLYAAWSLQENGADSSDLTELVLHTPVRSLSRAPARFDPAAGLP"
     gene            complement(1157..1690)
                     /locus_tag="VGD55_13895"
     CDS             complement(1157..1690)
                     /locus_tag="VGD55_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY0871487.1"
                     /translation="MTAAGDNPSETAQPGPDASKQPGTGPTQAGTQTHPRRDRRGRGI
                     RGPLAPAAVPLSRTRAAAFDELVLDAVEHLEHRWADQLSQVQFAVEDVPPVALLGRNT
                     IEPVPLGAWFAASGSRPGQIVVYRRPIEARSTGLEELAELVHDVVVEKVAEFLGLDPE
                     AVDPGYGEGWEPGSPEE"
     gene            1792..>1988
                     /locus_tag="VGD55_13900"
     CDS             1792..>1988
                     /locus_tag="VGD55_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HEY0871488.1"
                     /translation="MSPLRRCTRTACGQPAVATLTYVYAESTAVLGPLATYAEPHCYD
                     LCARHSARLTAPLGWEVVRLE"
BASE COUNT          276 a          672 c          698 g          342 t
ORIGIN      
        1 ggcccgcccg catcgggccc cgtcgccctt gccgcgaccg gacccgtgac cgactcgctc
       61 agtgcggtgc agttcacacg tgctccggtc aatggcgagc aacaggtctc gaccgcacag
      121 tgcgagagtc cgaccaccga cgcatggttc gcaggttttt cgagcaacgt tggcgcacat
      181 gcgacgttgt tgttgagcaa cgtcgactcg gtgccggcga cggtcgacgt ttcgatctgg
      241 ggcgcgcagg gcggtgagcc gaatgtccgc cgcgggatca ccgtcgatgc gctcacacag
      301 gtcgaggtgc cgctcgatca gatcgagccc ggacttcccg tcggtatcgc acacgtcgtc
      361 gcgacggccg gccgcgtggt gccggcgctg cgcggtgacg cagcgaacgg ctcgattccg
      421 ctgggcgtcg attggctgcc gccgaccgct gctcctgcga cgactttgac ggtgccgggc
      481 attctcggcg acattggttc gcgccggctc atcgtcggca atcccggcac agtcgatgcg
      541 acgttcgcac tgacggtggt gactgccgac ggtgcattca tgccgacgga gttcacttcg
      601 ttgacggtcg acgccgggga ggtgtccgac gtcagcctcg atccggtctt gcagggtgat
      661 gcggcagcga tcgtcgtcac gtcatcgcag ccggtcgttg cgggtgggat ttcggcgctc
      721 ccgctcgacg gtgccggtgg atcggacttc gccttcaccg gtgcggcggt gccgttggcc
      781 ggaccgacag ttgtggccgg gggagaggtc ggcgcaggaa ggcagaccca actcgtgatg
      841 tcggcgcccg gcgacgatgc gtcggtgcga gtggtgatcc tgccgtcggc ggtcgacgcg
      901 cctgaggttt cgaacgacat gacggtgacc gggggcacga ccacggtgct cgacctcgca
      961 gtgctgacga ccgacccggc gcccgcggtc gtcgtgatac ccgaaggtgg cgggccgctg
     1021 tatgccgcgt ggtcgctcca ggagaacggc gccgatagca gcgacctcac cgaactcgtg
     1081 ttgcacacgc cggtgcgctc gttgagtcgg gcgccggccc gcttcgatcc ggcagcaggc
     1141 ctgccgtagc tcggagctac tcctccggtg agcccggttc ccagccttcg ccgtagccag
     1201 ggtcgaccgc ttcgggatcg agaccgagaa actccgcaac cttctccacg acgacgtcgt
     1261 gaacgagttc ggccagttct tcgagcccgg tcgaacgagc ttcgatcgga cgccgataga
     1321 ccacgatctg acccggcctg ctgcccgatg cggcgaacca ggcgcccagc ggtaccggct
     1381 cgatcgtgtt gcggcccagc agcgcgacag ggggaacgtc ctcgaccgcg aattggacct
     1441 gactcaactg atcggcccac cggtgctcga gatgctcgac cgcgtcgagg accagctcgt
     1501 cgaacgctgc ggcccgggtg cgactgagcg gaacggcggc tggtgcgagt gggccgcgaa
     1561 tcccgcggcc gcgacggtcc cggcgcgggt gcgtctgggt gccggcttgc gtcggtccgg
     1621 tgccgggctg ctttgaggcg tccgggcccg gctgggctgt ttctgaagga ttgtcaccgg
     1681 ccgctgtcac ggctggcgag cctagtctcg atagagcggc ggccacgaca cgcggcgcga
     1741 tctcgggcgg atcgcccctc ctgacacatc gggcgactag ccttcgcgct cgtgagccct
     1801 cttcgccgct gcacccgcac tgcgtgcggc cagcccgccg tggcgacgct cacctatgtc
     1861 tatgcggagt cgaccgcggt gctcggcccg ctcgcgacct acgccgagcc ccactgctac
     1921 gacttgtgcg cccggcattc ggcccgcttg accgcgcccc tgggctggga ggtcgtgcgc
     1981 ctggagat
//
LOCUS       DASWVF010000784         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_200693, whole genome shotgun sequence.
ACCESSION   DASWVF010000784 DASWVF010000000
VERSION     DASWVF010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200693"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGD55_13905"
     CDS             complement(<1..255)
                     /locus_tag="VGD55_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871489.1"
                     /translation="MSDRTIISLEDFDRPTLVRWRNAIVAAFAVGGVALSTWGPRLPA
                     IRADLNLGTGGLGVLLAGITVGSVTGLLVSTPILGWLGPRR"
     gene            254..1051
                     /locus_tag="VGD55_13910"
     CDS             254..1051
                     /locus_tag="VGD55_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY0871490.1"
                     /translation="MAIIQSRRDRRSGMGSKARQKEILDALHRDGRVEVPELAERLGI
                     SAITIRRDLDQLAESGALRRVRGGAVTNSLRGEGLPFEVRAVDDSPLKARLAQAAVAL
                     IADGEAVAVDSGTTGAAAALALANRSVTAMPFSVQGIAALAAGSGVTLLLPGGSVRRP
                     EGTIVGPLAQQSLTDLRFDTAILTCCAASPTVGVTAYDVDDAAIKRALRHASARTILI
                     AEGAKFTRSAMAVVCRLDEVDVLVTDDSAPTEVLANLSEAGVHVVTA"
     gene            1391..3283
                     /locus_tag="VGD55_13915"
     CDS             1391..3283
                     /locus_tag="VGD55_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871491.1"
                     /translation="MACPDTTGDFDGEIHQQMDGAMSSCLQVGAVDAGAYTVAIDQVL
                     PDSGDTLGSPPATGAPEPSVHLSLSPNTGVPGTTVTVSGTSTAPLPQREDHGTLCWDG
                     CSDGLQYQGVELQWTSPTTFRTTMVVPAAPWVKGHPARVAMLASGTYSIGVLCLEQEK
                     GCALLGAQGSAPFHLQVPPSAVPTWCATQASCAELSISPTAALPGDVVKVTGFAPLTW
                     VIGSDQAYTYQLETRPGGPSASSVKFVDMNAKGEDAELGQGALTVLSAPPFASLKNTG
                     VVAGISDGFPAVVADPAHPTTHAWCDRSSVVVSSPNGTRMMPTAAASAALTRMGLPPY
                     DDSRPGCDAVAFVDDTVGAGSAALAIAFGVEPPNQDPMSGDVALTTRDGGRTWAPVPT
                     PRGAQAVGFGDFRYRDGALVAVFNPHDTGIADPPPLAETSRDGGRTWAPTTLSCPAAG
                     PCVTFGAFQSGNCAKGQATQPVLRSADDGRTWSEPPTLGWIGACAPAQLAATADGAEL
                     FVDNMSEFTLRRSTDGGATWADIAVPLLPDQQPGVGVGIGTDGITILPTGSLLATGQQ
                     DSGNDWLMLRSGTNAWCRVTTPGTGLQEGGNSTVTVIGDQLWWLVYDQDGAATPHRVE
                     IAALSC"
     gene            3372..3572
                     /locus_tag="VGD55_13920"
     CDS             3372..3572
                     /locus_tag="VGD55_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871492.1"
                     /translation="MTPTRTYRTDKLFAALLGYRSGLQPLWFDSAMDVDTARARSRER
                     IVAVHSTLARRRLRAVPGRRFS"
BASE COUNT          610 a         1259 c         1207 g          588 t
ORIGIN      
        1 acggcggggt ccgagccagc ccagaatcgg tgtcgagacc agcaggccgg tgaccgaccc
       61 gacagtgatg cccgcgagca gcacgccgag accgccggtg ccgaggttca ggtctgcacg
      121 tatcgcgggc aaacgtgggc cccaggtcga cagcgcaacg ccgccgacgg cgaacgccgc
      181 cacgatcgcg ttgcgccacc ggaccagcgt tggccgatcg aagtcttcca gggatatgat
      241 cgttcgatca gacatggcga tcattcaatc acggcgagat cggcggtcag gcatggggag
      301 caaggcccgg cagaaggaga tcctcgacgc gctgcatcgt gacggtcgag tcgaggtgcc
      361 cgagctggcc gagcgtctgg ggatctccgc gatcaccatc cggcgtgacc tcgatcagct
      421 cgctgaatcc ggtgccctgc ggcgcgtgcg gggtggcgca gtcacgaatt ctctgcgagg
      481 cgaggggctt ccctttgagg tccgcgccgt cgacgactct cccctcaagg cacgtctggc
      541 tcaggcggcc gtcgccctca tcgccgacgg tgaggcggta gcggtcgaca gcggcaccac
      601 cggtgcagct gccgcgcttg ccctcgcgaa ccggagcgtc acggcgatgc ccttctcagt
      661 gcagggcatc gctgcgctcg cggcgggttc cggcgtcacc ctgctgctac cgggtggctc
      721 ggtgcgacga cccgagggca cgatcgtcgg ccccctggcc cagcaatcac ttaccgacct
      781 gcgcttcgac accgcgatcc tgacctgctg cgccgcctcc cccacggttg gcgtcacggc
      841 ctacgacgtt gacgacgccg cgatcaagcg agccctgcgc cacgcctctg cgcgcacgat
      901 cctgatcgct gagggagcca agttcacccg cagcgccatg gcggtcgtct gccgcctcga
      961 cgaggtcgac gtgctagtca ccgacgactc agcgccgacc gaagtcctcg ccaacttgag
     1021 cgaggccggc gtccacgtcg tcacggcttg acgaaggatc tgggatcagc gctcggcgac
     1081 ctgcctgata ccaaagagca aatcggcggt acgcaaccaa acggctggct gcggcgtgtc
     1141 gtgtggcatg agggtgttgg ccttggtcgc cgtcacgttc gccgtcgcag cggggtgtgg
     1201 gagtcaaccg gcgccgccgc acgcaagcgg gccaccttcc gcgccagccg tctcatctgc
     1261 gccgcccgcc agttctgccc cggccgccat ctcatcaccg ccggtctcgg cgcctgctac
     1321 gtcaactctg aattcggaac cacttgcaca ggacggctcg ggtgacgaca caggaatgtc
     1381 gtggatgaac gtggcgtgcc ctgataccac tggcgacttt gatggcgaga tccaccagca
     1441 gatggacggc gcgatgtcga gctgcttgca ggtcggggcg gtagatgccg gcgcctacac
     1501 cgtggcgatc gaccaggtct tgccggacag cggtgacacc ctgggaagcc cgccggcaac
     1561 gggcgcgccg gaaccgtcgg tgcacttgtc gctgtcgccg aatactggtg tgcctggcac
     1621 aacggtgact gtgagcggca cgtcgacggc gccacttccg cagcgagaag accatggcac
     1681 tttgtgctgg gacggttgtt ccgacgggct ccagtaccag ggggtcgaac tgcaatggac
     1741 atcgccgacg acgtttcgga ccacgatggt ggtgccggcc gcgccctggg ttaagggtca
     1801 tccggctcgc gtagcaatgc tcgcgtcggg aacctactca atcggcgtcc tgtgcttgga
     1861 gcaggagaag ggctgcgccc tcctcggagc gcagggcagt gcaccttttc atctgcaggt
     1921 cccgccatca gccgttccga cgtggtgcgc aacgcaagcg tcgtgcgccg aactgagcat
     1981 ctcgccgaca gccgcgttgc cgggtgacgt cgtcaaggtc accggcttcg cgcctctcac
     2041 ctgggtcatc ggctccgatc aggcgtacac ctaccagctg gaaacgcggc ctggtgggcc
     2101 cagcgcatca tcggtgaagt tcgttgacat gaatgcgaag ggcgaggacg ccgagctcgg
     2161 tcagggagca ctgaccgtct tgtctgcgcc gccctttgca agtctgaaga acaccggcgt
     2221 ggtcgcgggc atcagcgatg gattcccagc tgttgttgca gatccggcac atccgacaac
     2281 acacgcgtgg tgtgaccgca gctcggtcgt cgtctcctcg ccgaacggca cacgcatgat
     2341 gccgacggcc gcggcgtcgg ccgcgttgac acggatggga ttgcctccct acgacgactc
     2401 gcgccctggc tgcgacgccg ttgcgtttgt ggatgacacg gttggagcag gcagcgccgc
     2461 gctggcgatc gcgttcggtg tcgagccgcc gaaccaagat ccgatgtccg gggacgtcgc
     2521 gctgacgacc cgcgacggcg ggcgcacgtg ggcaccggtg ccaactcctc ggggcgcaca
     2581 ggctgtcggc ttcggggatt tccgttaccg agacggcgcg ctggtcgcgg tcttcaaccc
     2641 acatgacacg ggaatcgctg atcctcctcc gcttgcggag accagccgcg acggtgggcg
     2701 cacctgggca ccgaccacac tcagctgccc ggctgctgga ccctgcgtca cattcggcgc
     2761 tttccagtcg gggaactgcg cgaagggcca ggcgacgcag ccggtgctcc gctcggccga
     2821 cgacggccgc acgtggagcg agccgcccac acttggctgg atcggagcgt gcgcgcccgc
     2881 gcagttggcg gctaccgccg acggagccga gttgttcgtc gacaacatgt ccgagttcac
     2941 gctgcgccga tcgacagatg gcggcgcgac ctgggccgac atcgctgtcc ccctcttacc
     3001 tgaccagcag ccaggcgtcg gcgtcggcat cggcaccgac ggcatcacga tcttgccgac
     3061 cggctcgctg ctcgcgaccg gccagcaaga cagcggcaat gactggctga tgctccgatc
     3121 cggcaccaac gcctggtgcc gcgtcacgac cccaggcacc ggcctccaag agggtggaaa
     3181 cagcaccgtc accgtcatcg gcgatcaact ctggtggttg gtctacgacc aagacggcgc
     3241 agcgacgccg caccgcgttg aaatcgcggc gctgagctgc tgaccaccgc gtgtatcagg
     3301 acggcgccgg tcgactcctc tttgtcgaga ccggacgcga taccagcgcg acaccaacaa
     3361 ggagagaccc cgtgaccccc acgcggacct accgcaccga caagctcttc gctgccctgc
     3421 tcggctaccg cagcggcttg cagcctctgt ggttcgactc ggccatggac gtcgacacag
     3481 cccgcgcgcg cagtcgcgaa cgcatcgtgg ctgttcacag cacgctggcc aggcgccggt
     3541 tgcgcgccgt ccctggtcgc cgattctcgt gaccgacgca gcgcgccgcg catgcgatta
     3601 tcacgaggtc cgcgcacgtc caacccggtc gatcaagccg agcaagcaga ggacctagcc
     3661 atgc
//
LOCUS       DASWVF010000785         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_149680, whole genome shotgun sequence.
ACCESSION   DASWVF010000785 DASWVF010000000
VERSION     DASWVF010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149680"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="truA"
                     /locus_tag="VGD55_13925"
     CDS             complement(<1..785)
                     /gene="truA"
                     /locus_tag="VGD55_13925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0871493.1"
                     /translation="MTGLAHSPKKADERKPEMVRLRIDLAYDGTAFSGWAKQPRLRTV
                     QGELEAALATVLRAPAPPQVTVAGRTDTGVHARGQVAHVDVDPASLVHLHPPTFARRL
                     NGLLESDVRIKAVSVAPEGFEARFAALSRRYSYRVADGWLDPLRRHDTLAHPKTLDLD
                     AMNAASTLLLGMHDFAAFCRRRVGATTQRTLLELTWSRDDEGVAIARVVADAFCHSMV
                     RALVGALLEVGDGRRDVRWPAAVLAGRKREDAAKVAPARGLTL"
     gene            complement(1193..1543)
                     /gene="rplQ"
                     /locus_tag="VGD55_13930"
                     /pseudo
     CDS             complement(1193..1543)
                     /gene="rplQ"
                     /locus_tag="VGD55_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1596..>2156)
                     /locus_tag="VGD55_13935"
     CDS             complement(1596..>2156)
                     /locus_tag="VGD55_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEY0871494.1"
                     /translation="PSHEIGRIPIDSIYSPVLKVTFKVEATRVEQRTDFDKLIVDVET
                     KPCMRPRDAVASAGKTLVELFGLARELNIDAEGIDIGPSPTDAALAADLALPIEDLNL
                     TVRSYNCLKREGIHTVGELVSRSEADLLDIRNFGAKSIDEVKAKLIEMGLNLKDAPPG
                     FDPTSVVYAGDDDDFDDQGYAETEQL"
BASE COUNT          312 a          713 c          740 g          391 t
ORIGIN      
        1 tcaagcgtga gcccacgggc cggcgccacc tttgcggcgt cctcgcgctt gcgtcctgcg
       61 agcaccgcag ccggccagcg gacgtcgcgg cgcccgtcgc cgacctcgag caacgcgccg
      121 acgagcgctc gcaccatcga atggcaaaac gcgtcggcga cgaccctcgc gatggccacc
      181 ccctcgtcat cgcggctcca cgtcagctcg agcagggttc gctgcgtcgt tgctccgact
      241 cgacggcgac agaaggccgc gaagtcgtgc atgccgagca ggagggtgga cgccgcgttc
      301 atcgcatcga ggtcgagtgt cttcgggtgg gccagcgtgt cgtggcggcg aagcgggtcg
      361 agccaaccgt cggcgacccg gtacgagtag cggcggctca gcgccgcgaa ccgagcctca
      421 aagccctcgg gagcgaccga tacagccttg atgcggacgt cggactcgag caggccgttg
      481 agccgccgcg cgaacgttgg tgggtgaagg tgcacgagag atgccgggtc gacgtcgacg
      541 tgagccacct ggccgcgcgc gtgcaccccg gtgtcggtac gcccagcgac ggtgacctga
      601 ggtggcgcgg gtgcgcgcag cacggtcgcc agcgcggcct cgagctcgcc ttgcaccgtg
      661 cgcaggcgtg gctgcttcgc ccagcccgag aatgccgtgc cgtcgtaggc aagatcgatc
      721 cggagtcgaa ccatctcagg cttgcgttcg tcagcctttt tgggggaatg agcgagcccg
      781 gtcatcccgt gatcgggagg accgggctcg tcgctgtcgt ccgggccacg gtgacggctc
      841 aggcctggtc gtcctcggca gccttctcgg cagcctcggc cgcttcatct gcaatctctt
      901 cgaacgcgat tgcctcgccc gcgaccgcag ccgacgcatc gtcgccgatc tcgccggcaa
      961 gttcctctgc cttcgcggcg gcctcgtcgg cttctgcggc cgcaagcctg gcaccctcga
     1021 cgtcaccctc ggcctcggca tcctcagcag cctcggccgc ttcttctgcc ttggcttgga
     1081 gcgcttcgat ttcggcgacg cgcgcgggag ccgccgtcgt cttcgtggca cgttccttgg
     1141 ctgcgcgttt ggttgcaccg cgagcctcgg ccacgacctg ttcagccagg gcctcgacca
     1201 gctcgatgac cgccatgggc gcgttgtcgc ccttgcgcgg cccgagcttc acgatgcggg
     1261 tgtagccacc tggtcggttc gcatagcgcg gaccgatctc agtgaatagg ccatgcacca
     1321 cggtcttgtc gcggacgacg gtcatgacct gtcggcgcgc atgcaggtca ccacgcttcg
     1381 ccttggtgac gagccgctcg gccacaggac gcagccgctt tgccttggcg agagtggtgg
     1441 tgatgtggcc gtgctcgaac aacgccgtcg cgaggttcgc gaggatcagc cgctcgtgcg
     1501 ctgagcctcc gccgaggcgg ggtcccttgg tgggcgtggg catgtccggg ccttcctaga
     1561 tcgaatgcgt gtaaaacagc tcagtgctgc ggtggctaaa gctgctcagt ctcggcgtat
     1621 ccctggtcgt cgaagtcgtc gtcgtcgccg gcgtagacca cgctggtcgg gtcgaatccg
     1681 ggaggcgcgt ccttcaggtt caggcccatc tcgatgagct tggccttgac ctcgtcgatc
     1741 gacttcgcac cgaagttacg gatgtcgagc aggtcggctt cgctgcgcga gaccaactca
     1801 ccgacggtgt ggatgccttc gcgcttgagg cagttgtacg aacgcacggt caggttgagg
     1861 tcttcgatcg gcaacgcgag gtcggcagcg agcgcagcgt ctgtcggcga cgggccgatg
     1921 tcgatgccct cggcgtcgat gttcaactcg cgggcgagac cgaacaactc gacgagcgtc
     1981 ttacctgcac tggcgacggc gtcgcgcggg cgcatgcacg gcttggtttc gacgtcgacg
     2041 atgagcttgt cgaagtcggt gcgctgctcg acacgggtcg cctcgacctt gaacgtgacc
     2101 ttcaagaccg gggagtagat cgagtcgatc gggatgcggc cgatctcgtg gctggg
//
LOCUS       DASWVF010000786         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_22591, whole genome shotgun sequence.
ACCESSION   DASWVF010000786 DASWVF010000000
VERSION     DASWVF010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22591"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VGD55_13940"
     CDS             complement(<1..1362)
                     /locus_tag="VGD55_13940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0871495.1"
                     /translation="MAYHWSIHQLTEYFEAVTSQDDEFVAVRGAIERAVEALGAEVGA
                     LVRDDDLLACVGMGRDVPPVEVMRPAAQGAVMIDMPGLGLLHTAAAQLDSDISGCLVL
                     GRIHDEFTGEERQLLQGMASVLGLALRSLRTLQVERTLRTEREQEASERLVLLDTLGR
                     RQRLLESLLTIQRAISHRAPSQTVLDAVTAGAAQLLNDAFVALVIVEPGVGDSRVVSS
                     GGLMSGADSYRSLIMAAAADSVAIDGLVTRVSDGDDGQCTALAAPVHIEGNTTGSLVT
                     VALPGAADVNDRRDTLAAFAEQASVALTDARTVEAMRDAYRDSLTGLPHRALFLDRLN
                     HALLTAARLGTDVTVLFIDLDRFKDVNDSLGHAAGDELLAWFADRIREALRGDDTAAR
                     LGGDEFAILLERTEGDAAGRRVAARLTEALLEPVWLAGREVFAFASIGIATSDTSTGT
                     ADDL"
     gene            complement(1373..2608)
                     /locus_tag="VGD55_13945"
     CDS             complement(1373..2608)
                     /locus_tag="VGD55_13945"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0871496.1"
                     /translation="MTAVLNETVPTQTAFTGFKGPQAASRWFGVGRSTDSDVRVAGAD
                     ATREALAGRSASLVVVYCPADIDFRAMLDGVRSAAGDVPLIGCSGLAQLAQSGAADPA
                     VVVSALGGNGFEVRTSVALNVSGGQRAAGEHVADIVSGLTSEHKFLMLLADGLAGDQH
                     ELVRGAYNVVGATVGLAGGCAADDFLYEQTFQFIGDATGVQVLSNAVVAAAIGSNAPI
                     GVGVAHGWRKVGEPMVATRSAGGIVYTLDGEPALDVFAKRVGAPRSIAEDSEAFIVFA
                     MKHPLGMSRRSGEDIRVIHGGNAKDGSIECLADVPQGALLWMMETDRAGLVSCVDDAY
                     AEAVAQLDDVPPLGFMVFDCGVRYLFLEPEGVREEVARFTDRTAGIPFAGFYTMGEIA
                     RTRGARGMHHLTLVLVAFA"
     gene            complement(2721..>3499)
                     /locus_tag="VGD55_13950"
     CDS             complement(2721..>3499)
                     /locus_tag="VGD55_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0871497.1"
                     /translation="SRLNLAAGDWVSVSPLFAQALRVACDAAQWSDGLVDPTVGRALI
                     DLGYDRTFTLVAADGPPLTVVARAVPGWRQLEVDDEGLRARVPVGVRLDLGASAKSLA
                     ADLSAAAVAADVGCGVLVSLGGDIAVAGDAPVGGWPIDVTDCSDPELPAGGAGQIVAI
                     RGGGLATSSTRARRWRRGGSELHHLIDPRSGLPAAAPWRTVSVAAPTCVLANVASTAA
                     MIIGTSAAGWLSGRGFSARLVAVGGTPAYVGGWPTGAERS"
BASE COUNT          653 a         1231 c         1086 g          529 t
ORIGIN      
        1 aaggtcgtca gccgtgccag tcgaggtatc actcgttgca attccgatgc tcgcgaacgc
       61 gaatacctcc cgaccggcca accacacggg ctccagcagt gcttccgtca gccgggctgc
      121 gacccggcgt ccggccgcgt cgccttcggt tctttccagc aggatcgcga actcgtcgcc
      181 gcccagacgt gcagcggtgt cgtcgccccg caatgcctcg cggatccgat ccgcgaacca
      241 cgcgagcaat tcgtcgccgg cggcgtgtcc gaggctgtcg ttgacgtcct tgaatcggtc
      301 gaggtcgatg aacagcacgg tgacgtcggt acccaatcgc gcggccgtca gcaaggcgtg
      361 attgagacga tcgaggaaca aggcgcgatg cggcagcccc gtcaacgagt cgcggtacgc
      421 gtcacgcatt gcttcgaccg tgcgcgcgtc ggtgagcgcg acgctggctt gctcggcaaa
      481 ggccgccagg gtgtcacgcc ggtcattcac gtccgctgcg cctggcaacg cgaccgtcac
      541 gaggctgccc gtggtgtttc cctcgatgtg caccggcgcg gctagcgccg tgcactgccc
      601 atcgtctccg tcgctcaccc tcgtcacgag accgtcgatc gcgacggaat cggcggccgc
      661 cgccatgatg agagagcggt agctgtcggc accgctcatc agtccgcctg aagacacgac
      721 gcgactgtca cccacccccg gttcgacgat cacgagagcg acaaaggcgt cgtttaggag
      781 ttgtgcggcg ccagcagtga ctgcgtcgag tacggtctgc gacggcgcgc gatgcgagat
      841 agcccgctgg attgtaagca acgactcgag caaccgttgc cgacggccga gcgtatcgag
      901 caataccaaa cgctcagacg cttcctgctc gcgctcggtt cttagcgtcc gctcgacctg
      961 gagagtgcgc aagcttcgca gagcgagacc caggacgctc gccattccct gcaacagttg
     1021 gcgctcctcg ccagtgaact cgtcgtggat ccgaccgagc acgaggcacc ccgagatgtc
     1081 gctgtcgagc tgagcagcgg ccgtgtgcaa caagccgagc cccggcatgt cgatcatcac
     1141 ggcgccctgc gctgcaggcc tcatgacctc gaccggcgga acgtctcgac ccatgcccac
     1201 gcacgcaagc aaatcgtcgt cacgaacgag cgccccaacc tcggcaccaa gggcttcgac
     1261 cgcgcgctcg atggctccgc gtacggcgac gaactcgtcg tcctgcgaag tgactgcttc
     1321 gaagtattcg gttagctggt ggatggacca gtgataagcc accggctgcc cctcatgcga
     1381 aggccaccag caccaacgtc aggtgatgca tgcctcgtgc tccccgagtg cgcgcgattt
     1441 cgcccatcgt gtagaagccg gcgaacggta tcccggcggt gcgatcggta aaccgtgcaa
     1501 cctcctcgcg aacgccctct ggctccagga agaggtagcg gacaccgcag tcgaacacca
     1561 tgaacccaag aggcgggacg tcgtccagct gggcaactgc ttcggcgtaa gcgtcgtcaa
     1621 cgcaggagac caagcctgcg cggtcggtct ccatcatcca gagcagcgca ccctgcggaa
     1681 cgtctgcgag gcactcgatg gagccgtctt tggcattgcc gccgtggatc acccggatgt
     1741 cctcgccgct gcgacgcgac atccctaatg gatgcttcat cgcgaacacg atgaaggcct
     1801 cactgtcctc cgcgatcgac ctcggcgcgc ccactctctt ggcgaacacg tcaagtgcgg
     1861 gctcgccgtc gagcgtgtac acgatgccgc cggcactgcg agtcgccacc atgggctcac
     1921 ccaccttgcg ccagccgtgg gcaacaccga cgccgatcgg agcattagaa ccgatcgccg
     1981 ccgctacgac tgcattgcta agtacctgca ctccagtcgc gtcaccaatg aactgaaacg
     2041 tctgctcgta caggaagtcg tcggcggcgc agccgcccgc tagtccaacg gtcgcgccca
     2101 ccacgttgta ggccccgcgg accagttcgt gctgatcgcc ggcgagtcca tcggcgagca
     2161 acatcaggaa cttatgctca ctcgtgagac cgctcacgat gtcagcgacg tgctctcccg
     2221 cggcacgttg gccgccggag acgttcaggg ccacgctggt gcggacctcg aagccgttgc
     2281 ctcccaacgc acttactacg acagcaggat ccgcggcacc ggactgtgcg agctgcgcta
     2341 aaccgctgca accgatcaac ggtacgtcgc cggccgcgga cctaactccg tcgagcatgg
     2401 ctcgaaagtc gatgtcagct ggacagtaga cgacgaccag ggatgcggac cgacctgcca
     2461 atgcctcgcg ggtcgcgtcg gcgccggcca cccgaacgtc gctgtcggtg ctgcgaccca
     2521 cgccgaacca gcggctggcg gcttgcggcc ccttgaatcc ggtgaaagcg gtctgggtgg
     2581 ggaccgtctc gttcaacaca gccgtcatgc gtttgcctcc aaggcgcccc gcgcacgagc
     2641 tctgggcgag ctcatggaga ccgaatcgac ctggcaacgc ccgaattgag gaattcaggc
     2701 gcggacgacg gcggcgaacg tcacgaccgc tcggcgccgg ttggccaccc gccgacgtag
     2761 gcaggcgtgc cgccgacggc caccaaacgc gccgagaacc cgcggcccga aagccagccg
     2821 gccgccgacg tgccgatgat catggcggcg gtcgaggcca cgttggcaag cacgcaggtc
     2881 ggcgcagcga cgcttaccgt gcgccaggga gcggcggccg gcaggccgct tcgagggtcg
     2941 atcaagtgat gcaactccga tccccctcgg cgccagcgtc gcgcccgagt gctcgacgtc
     3001 gccagcccac caccgcggat cgccacgatc tggccggcgc cgcccgcagg tagttcgggg
     3061 tcgctgcagt cggtcacatc aatcggccag cctccaactg gagcatcgcc ggcaaccgcg
     3121 atgtcgccac caaggctgac aagcactccg caaccgacgt cagccgcaac cgcagcggca
     3181 gacagatcgg ccgctaacga tttcgcggaa gcaccaagat cgagacggac gccgacgggc
     3241 acccgcgccc gcagcccttc atcgtcgacc tcgagctgac gccaaccagg cacagcgcgc
     3301 gcaaccacgg tgagtggcgg accgtctgcg gcgacgagcg tgaaggtgcg gtcgtacccg
     3361 aggtcgatca gcgccctccc gaccgtcgga tcgaccagac cgtcagacca ctgcgccgca
     3421 tcgcacgcga ctcgaagagc ctgcgcaaac agtggcgaca ccgaaaccca gtcgcccgcg
     3481 gccaggttga gccgactca
//
LOCUS       DASWVF010000787         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_481476, whole genome shotgun sequence.
ACCESSION   DASWVF010000787 DASWVF010000000
VERSION     DASWVF010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481476"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..447)
                     /locus_tag="VGD55_13955"
     tRNA            complement(375..447)
                     /locus_tag="VGD55_13955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Asn,seq:gtt)
     gene            623..934
                     /locus_tag="VGD55_13960"
     CDS             623..934
                     /locus_tag="VGD55_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871498.1"
                     /translation="MRYKATLVIGLAAGYVLGARAGRQRYEQIKRLTQTVKKNPAVQN
                     VAGKVQAQANQLSTQARRVVQDKAGSVGHDLVGKVTSRFGSHTITLDDEDRAYSNGTL
                     S"
     gene            complement(935..>2357)
                     /locus_tag="VGD55_13965"
     CDS             complement(935..>2357)
                     /locus_tag="VGD55_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0871499.1"
                     /translation="LDERGFDSAAAAHFGVGYAPNGWEHLVKHLRGRGFTDEELATGG
                     LASRGQRGVIDRFRGRLVWPIRDVTGDVVGFGARKLRDDDDGPKYLNTPESPLYKKSQ
                     VLYGIDLAKREMAKARQAVVVEGYTDVMACHLAGVTTAVATCGTAFGDEHVRVLRRLL
                     LDSAEYAGEVIFTFDGDSAGQKAALRAFESDQKFVTHTFVAISPDNMDPCELRIAKGD
                     AAVRDLVARRQPLFEFAVRSVLAGFDLETSEGRVGALDRTMPLVASIKDTSLRDEYAR
                     RLAGWVGAPDELGVLERVRRIAGTSSSRQSRSAAADRPDPRDPTLQVEREALKLAVQR
                     PALLGPAFDLLDPLAFTHLAYAEVRDAVAKAGGTGSTAGGDAWVTAVLEAAVDDRVRA
                     LITELVVEPVLTAIDPDERYARAQLAQLRVRALDRQIAEVKSRLQRLNPLEHVTDYNR
                     LFGDLVMLEKRRRDDLDGTLGVA"
BASE COUNT          419 a          798 c          769 g          371 t
ORIGIN      
        1 gctcctcgct ccaccggtta aaccggcgca acgcagtccg aaagcgcaac agcgcggcaa
       61 aatccaatgc cgcgtcggtc gcaggccccg gtccgggatc ggacgccact atgcgcttag
      121 atgccatcga cgaggaccct acgcgcttgg ctctcattct caatcgacag acacgttggc
      181 tgacgctctc agcaaatgcc atggcgtcgt cggatacgcg tgggatgcgg cgcagcagat
      241 cgacggcgag ccgcgttcgc agaagtcttg atcaccgcgc ccgtcaccaa actcgtccgc
      301 catcgccgcc gtgctggttc acctggacgc gatcatctcc ccgctcggtg caggtcagag
      361 gctgacttgc gatgctcctg cgataggact cgaacctata acctgccggt taacagccgg
      421 ctgctctgcc aattgagcta cgcaggaatg acggtcggcg taccggccgc tggtgcctcc
      481 gtagggtatc gcacggcgag accgcgcccg ggagcctcgg accagtcctc gacgcaccct
      541 cgaccacccg gccggctggg gatacggtcg gagtctcgcg ggtatgcacc agtgagaacg
      601 acgtctaacc cgaggagcgc gcatgcgata caaggccact ctcgtcatcg gactcgccgc
      661 cggatacgtc ctcggcgccc gcgctggtcg gcagcgctac gagcagatca agcggctgac
      721 ccagacggtg aagaagaacc ccgccgtcca aaatgtcgcg ggcaaggtgc aggcgcaggc
      781 caaccagctc agcacccagg cccgccgcgt cgtgcaagac aaagctggct cggtcggcca
      841 cgatctcgtc ggcaaggtga cgagccgttt cggcagccac acgatcacgc tggacgacga
      901 ggaccgcgcc tactcgaacg gcaccctgag ctgactaagc cacaccgagc gtgccgtcga
      961 gatcgtcgcg ccggcgcttc tccagcatga cgagatcgcc gaacaggcgg ttgtagtcgg
     1021 tgacgtgctc gagcggattc agccgttgca accgcgactt cacctcggcg atctgccggt
     1081 cgagcgctcg cacgcgcagc tgcgcaagtt gcgcgcgggc gtaccgctcg tcgggatcga
     1141 tcgccgtcag caccggctcg acgacgagct cggtgatcag cgcacgcacc cgatcgtcga
     1201 ccgcagcttc gaggacggcg gtcacccatg cgtcaccgcc tgcggtcgac ccggtgccac
     1261 ccgccttcgc aaccgcatca cgcacctcgg cgtaggcgag atgagtgaac gccaacgggt
     1321 cgagcaggtc gaacgcagga ccaagaagag cagggcgttg gacggcgagt ttcagcgcct
     1381 cgcgttcgac ctgcaacgtc ggatcgcggg gatctggccg gtcggcggca gcgctacggg
     1441 attgccggct gctcgacgtc cctgcaatgc gacgcacgcg ctcgaggacg ccaagttcat
     1501 cgggggcgcc gacccagccg gctaggcggc gggcgtattc gtcgcgcaac gacgtgtctt
     1561 tgatcgaggc gaccagcggc atggtgcgat cgagcgcgcc gacgcggcct tcggaggtct
     1621 ccaggtcgaa gcccgcgagc acactgcgca ccgcgaactc gaacaacggc tggcgtcggg
     1681 caaccaggtc gcgcacggcc gcgtcgcctt tggcgatacg caactcgcac gggtccatgt
     1741 tgtcggggct gatcgcgacg aacgtgtggg tgacgaactt ctggtcgctc tcgaacgcgc
     1801 gcagtgcggc cttctgcccg gcggagtcgc catcgaacgt gaagatgacc tcgccggcgt
     1861 attcggcgga gtcgagcaag agccgtcgca gcacccgcac gtgttcgtcg ccgaacgcag
     1921 tgccgcaggt cgcgaccgcc gtcgtgacgc cggcgaggtg gcaggccatg acgtcggtgt
     1981 agccctcgac gacgacggct tgccgcgctt tcgccatctc gcgcttcgcg aggtcgatgc
     2041 cgtagagcac ttgacttttc ttgtatagcg ggctttctgg agtgttcagg tacttgggac
     2101 cgtcatcatc gtcgcgcagc ttgcgcgcac cgaaccccac gacgtcaccg gtgacgtcgc
     2161 ggatgggcca gacgaggcgc ccgcggaacc ggtcgatgac accgcgctga ccgcgactgg
     2221 ccaagccgcc ggtcgcgagc tcctcgtcgg tgaatcctcg accgcgcagg tgcttgacga
     2281 ggtgttccca accgttgggt gcatagccga ccccgaagtg cgcagcggct gctgaatcga
     2341 acccgcgctc gtcgaga
//
LOCUS       DASWVF010000788         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_353823, whole genome shotgun sequence.
ACCESSION   DASWVF010000788 DASWVF010000000
VERSION     DASWVF010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353823"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..998
                     /locus_tag="VGD55_13970"
     CDS             9..998
                     /locus_tag="VGD55_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0871500.1"
                     /translation="MGVTSPFVLAVTVTSFNPANPLTGLTVDEVPDPTPPDGWVRVAV
                     KAAALNHHDLWSLRGVGLRLDDLPRILGCDAAGIDEDGNEVVVHAVIGDPDAGRGDET
                     MDPARTLLSERIDGALAEFVVVPARNLVPKPEGLTFEEAACLPTAWLTAYRMLFTNSG
                     TRPGDTVLIQGAGGGVSTALIALSKAAGRRVWVTSRSLDRGGLALKLGADAAYESGGQ
                     LPERVDAVFDSVGAATWAHSLRCVRPGGTIVTCGATSGDAPSAELSRVFFRQLRVIGS
                     MVGARHELVELLNFLVQTGVRPIIDRALPLMSAREGFEALAAGEVFGKVVFTV"
     gene            complement(1192..1374)
                     /locus_tag="VGD55_13975"
     CDS             complement(1192..1374)
                     /locus_tag="VGD55_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HEY0871501.1"
                     /translation="MYFTDRGIEELETRRGDESVTLAWVAEQLRTFVDLNPEFEIPLE
                     RLATWLARLDDDPDAD"
     gene            complement(1378..>2861)
                     /locus_tag="VGD55_13980"
     CDS             complement(1378..>2861)
                     /locus_tag="VGD55_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0871502.1"
                     /translation="RLALEFRQAFKKDVVIDLVCYRRRGHNEADDPSMTQPLMYNLID
                     SKRSIRKLYTEALIGRGDITLDEAEAALRDYRGHLEKAFTETRDSTSAPHEPTVVENA
                     PAASPDVQTAISVEVLKKIANSQINLPPSFIAHPRLLPQLQRRAAMVDDDAIDWAMGE
                     LIAIGSLLLDGHPVRLAGQDTRRGTFTQRHAVLIDRENGSEYTPLANLDPAQAPFYIY
                     DSLLSEYAAVGFEYGYSVVRPEALVAWEAQFGDFADGAQTILDEFISSGEQKWGQQSG
                     VALLLPHGYEGQGPDHSSARVERYLQLCAENNMTVAMPSCSASYFHLLRWHTKSPHHR
                     PLIVFTPKSMLRLKAAASSAAAFTAGAWAPVLPDPRPQPAAGVRRLILCSGKVTWDLV
                     DAREQRGRSDTAILRVEQLYPLPVAELAAAASEYPNMQEILWVQEEPANMGAWSHIAM
                     RLHAQLGMPITPVTRPESSSPATGSHERSEHETKVLLAAAFPE"
BASE COUNT          443 a          884 c         1020 g          514 t
ORIGIN      
        1 ccgggtggat cggcgtaacg tcaccttttg tgctcgccgt cacagtgacc tccttcaatc
       61 ccgccaatcc gttgaccggt ctgacggtcg acgaggtacc ggatccgacg cctcccgacg
      121 ggtgggtgcg ggttgcggtc aaggcggccg cgctcaacca ccacgacctg tggagcctgc
      181 gcggggtcgg gctgcgactc gacgacttgc caagaatcct cggctgcgat gctgccggca
      241 tcgatgaaga cggcaacgag gtcgtcgtcc atgccgtcat cggtgacccc gacgcgggcc
      301 gcggcgacga gaccatggac ccagcgcgga ccctgctctc ggaacgaatc gacggtgcac
      361 tggccgaatt cgtcgtggta cctgcgcgca atctggtgcc caagcccgaa ggtctgacct
      421 tcgaggaggc tgcttgcctc ccgaccgcat ggttgaccgc ctaccgcatg ctgttcacga
      481 actcaggcac tcgcccgggt gacacggtgc tgatccaagg agcgggcggc ggggtctcga
      541 cggcactgat cgcgctttcc aaagcagccg gacgccgggt gtgggtgacg agccgatcac
      601 ttgaccgcgg tggccttgcg ttgaaactgg gtgccgatgc ggcctatgag tcgggcggtc
      661 agctgccgga acgcgtcgat gcggtgttcg acagcgtggg cgcagcgacc tgggcgcatt
      721 cgctgcgttg cgtgcggccc ggcggcacga ttgtgacgtg cggcgcgacc agcggcgacg
      781 caccctcggc cgagcttagc cgggtgttct tccgacaact gcgggtgatc ggttcgatgg
      841 tcggcgctcg gcacgagctg gtggagctgc tgaacttcct ggtgcaaacg ggcgtgcggc
      901 ccatcatcga ccgcgcgctg ccgctgatgt ccgcgcgcga gggctttgag gcgctggccg
      961 cgggtgaggt gttcggcaag gtcgtcttca cggtctgatg gtcgacggtg agggttggcg
     1021 tggccggcgg gtcagcggac agcatgtcgc gagcaactca acgtggagta tgggcgtcgc
     1081 tagaacgacg ccggcgctcc acaatccggt ctgccgggtt gcgacggtgg gatggtggga
     1141 cggcgggatg gtgggctggc gggatggagc cgcgcaagcg atcggcggag cctagtcggc
     1201 gtccgggtcg tcgtcgaggc gagcaagcca ggtggctagc cgctcgagcg gaatctcgaa
     1261 ctcgggattg aggtcgacga acgttcgcag ctgctcggca acccaggcca gcgtgaccga
     1321 ctcatcgccg cggcgcgtct cgagctcttc gatgccgcgg tcggtgaagt acatgcccta
     1381 ctccgggaac gcggcggcga gcagcacttt ggtttcgtgc tcgctgcgct cgtgcgaccc
     1441 ggtcgccggc gacgagctct ccggccgggt aaccggcgtg atcggcatcc cgagctgtgc
     1501 gtgcagtcgc atcgcgatgt ggctccacgc gcccatgttc gccggctctt cttgcaccca
     1561 gaggatctct tgcatgttcg ggtactccga agcggcggca gcgagctcgg cgaccggcag
     1621 cgggtagagc tgctcgacgc gcaggatcgc agtgtcgcta cggccgcgct gctcgcgcgc
     1681 gtcgacgagg tcccacgtca ccttgccact gcaaagaatg agacgccgga cgccggctgc
     1741 cggctgcggg cgtgggtcgg gaagcaccgg cgcccaggca cctgcggtga aggcggccgc
     1801 cgaagacgcg gcggccttga gccgcagcat cgacttgggc gtgaagacga tgagcggacg
     1861 gtggtgcggg ctcttggtgt gccagcgcag cagatggaag taactcgcgg agcacgacgg
     1921 catggcaacc gtcatgttgt tttcggcgca caactggagg taacgctcga cgcgggccga
     1981 cgagtggtcg gggccctggc cttcgtaacc gtgcggcagc agcaacgcga cgcctgattg
     2041 ctggccccac ttctgctcac cggaggagat gaactcgtcg aggatcgtct gagctccgtc
     2101 ggcgaagtcg ccgaactgcg cctcccacgc gaccagtgct tcggggcgca cgaccgaata
     2161 tccgtactcg aatccgacgg ccgcgtattc ggacagcagc gagtcgtaga tgtagaaggg
     2221 cgcttgggcc gggtcgagat tggcaagcgg cgtgtactcg ctgccgttct cccgatcgat
     2281 caagaccgcg tgccgttggg tgaaggtgcc tcggcgagtg tcttgcccgg ccagtcgaac
     2341 cgggtggcca tcgaggagca acgagccgat cgcgatcagc tcacccatcg cccagtcgat
     2401 cgcgtcgtcg tcaaccatcg cggcgcgtcg ttggagctgt ggcagtaacc gcgggtgggc
     2461 gatgaaactt ggcggcagat tgatctgtga gttggcgatc ttcttcagaa cctcgacgct
     2521 gattgccgtc tgcacatcgg gcgacgctgc cggggcgttc tctacgactg tcggctcgtg
     2581 gggtgcgctt gtcgagtcgc gcgtctcggt gaacgccttc tcgaggtgtc cgcgatagtc
     2641 gcgcagcgcc gcctccgcct cgtcgagcgt gatgtcgccg cggccgatga gtgcctcggt
     2701 gtacagcttc ctgatcgatc gcttcgaatc gatgaggttg tacatcagcg gctgcgtcat
     2761 cgatgggtcg tcggcttcgt tgtgaccgcg ccggcggtag cagaccaagt cgatcacgac
     2821 gtccttcttg aacgcctggc ggaattccag cgcgagccgc g
//
LOCUS       DASWVF010000789         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_430084, whole genome shotgun sequence.
ACCESSION   DASWVF010000789 DASWVF010000000
VERSION     DASWVF010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430084"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1973
                     /locus_tag="VGD55_13985"
     CDS             <1..>1973
                     /locus_tag="VGD55_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0871503.1"
                     /translation="VDLDEALELVFEAERRLGAVQLALAVTAADRAVEILARGVVLDD
                     EGDLLPHGPDEARRLLALQRARYAGAAAALQADDVAAALRFADAAVADDRVDETAHRL
                     LMAAHQAAGEPARAIASYTQLRDVLDAELGVEPAPATRDLYVAILREQDARPGVAVET
                     QHVPQGSGPRLVGRAAEVARLEAAWSAASSGSPDLVLLVGQAGIGKSRLSREAAVIAT
                     ATGGTVLHARCYETERAMFLQPVIEALTPALERLSSADLQRVAGDDSATLAALVPSAL
                     GVVRTTTALRGDVDLERRRVFDAVTDTIGRLAERAPVLLVLDDLQNAGRATIELLHYM
                     TRRLSGSRVLLLGVVRREEGHDAIDALTAVATVIEVNSLSYAAVADLAGAFGQAHLVD
                     HILRQTGGHTLYVIETLGALASGQSGLPPSLHDAVVDRVSRLGPDVDTLLRAASVLGA
                     TLHVEHLAAILAITTPLVLDRCQRAYAAGLLVVAEREYEFANDLVREVLYESTPEPTR
                     RAWHLHAADLLTDQPEAMAGHAAAAGDQGRAARAWLLAAHEAFRRFAAADAEALATTA
                     IDAATLVSDLDLAARGYILRGRAREITTDFAAALSDLETAVALARESGDQRVEMIALR
                     QLGGDVPVALRLSVAPGISHLERGLFLAEGLGDR"
BASE COUNT          286 a          676 c          682 g          329 t
ORIGIN      
        1 gtcgatcttg acgaggctct cgagttggtt ttcgaagcag aacgccggct gggcgccgtc
       61 cagctcgccc ttgctgtgac tgccgcggat cgggccgtcg agatcctcgc gcgcggtgtg
      121 gtgctcgacg acgagggtga tcttctgccg cacggtcccg acgaagcgcg ccggttgctg
      181 gcattgcaac gtgcgcgcta cgccggggcg gcggcggccc tacaggctga cgacgttgcc
      241 gcggcgcttc gcttcgccga cgccgccgtc gctgacgatc gggtagacga gaccgcgcat
      301 cggctgttga tggcggccca ccaagcggcg ggcgagccgg cccgggcgat cgcgagctac
      361 acccagttgc gcgacgtcct cgacgccgaa ctaggtgtcg agccggcacc cgccactcgc
      421 gacctgtacg tcgccatctt gcgggagcag gacgcgaggc cgggcgtcgc ggtcgagacg
      481 caacacgtgc cacaagggag tggcccgcgg ctggtcggcc gcgcggccga ggtggcacga
      541 ctcgaagcgg catggtcggc ggcaagctcg ggctcgcccg acctcgtgct gctcgtaggg
      601 caggcaggga tcgggaagtc tcgcctctcg cgtgaggcgg ccgtgatcgc gaccgctacg
      661 ggcgggaccg tcctgcacgc gcggtgctac gagaccgagc gcgccatgtt tcttcagccc
      721 gtgatcgagg cattgacgcc cgctctcgag cggctctcgt cggcggactt gcaacgcgtc
      781 gctggcgacg actcggcaac cctcgcggca cttgtgccat cggcgcttgg cgtggtgcgc
      841 acgaccactg cgctgcgggg agacgtcgac cttgagcgcc gacgcgtgtt cgacgcggtc
      901 accgacacca tcggtcgact cgccgaaagg gcgccggtgc tgctggtgct cgatgacctg
      961 cagaacgctg gtcgagccac catcgaactg ctccactaca tgactcgccg gctgtcaggt
     1021 tcgcgggtct tgctcctagg cgtggtgcga cgagaagagg gtcacgacgc gatcgacgcg
     1081 ctgacagcgg tcgcgaccgt gatcgaggtg aactcgctgt catacgcggc cgtcgccgat
     1141 ctcgcggggg cgtttgggca ggcacatctg gtcgatcaca ttcttcgcca gaccggtggt
     1201 cacacgctct acgtcattga aacactcgga gcgctcgcct ccggtcagtc gggactgccg
     1261 ccgtccctgc atgacgccgt cgtcgatcgc gtcagccgtc taggccctga cgtcgacacg
     1321 ttgctgcgcg cggcgtcagt gctcggcgcg acgctccacg tcgagcatct agcggcgata
     1381 ctcgcgatta cgacaccctt ggttctcgac cgctgccaac gggcgtacgc ggcaggcctg
     1441 ctcgttgtcg cggagcgaga gtacgagttc gccaacgacc tcgttcgcga ggtgctgtac
     1501 gagtcgacgc cggaaccgac tcgtcgcgcg tggcacctcc acgctgccga cttgctcacc
     1561 gatcagcccg aggcaatggc cggtcacgcg gccgcggcgg gtgaccaggg tagagccgcc
     1621 cgggcgtggc tgctcgccgc ccacgaggca tttcgacggt tcgctgccgc cgacgccgaa
     1681 gccctggcga ccacggcgat cgacgccgcc acgctcgtgt ccgacctcga cctcgccgcg
     1741 cgtggctaca tccttcgcgg ccgggcgcgt gagattacga ccgacttcgc agcagcgtta
     1801 tccgacctag agacggccgt tgccctggcc agagaatcgg gtgaccagcg ggtcgagatg
     1861 atcgcgctgc ggcagctcgg cggcgacgtc cccgtcgcgc ttcgactctc ggtcgcaccc
     1921 gggatcagtc atctcgagcg cggcctcttt cttgccgaag gcctgggtga tcg
//
LOCUS       DASWVF010000790         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_456084, whole genome shotgun sequence.
ACCESSION   DASWVF010000790 DASWVF010000000
VERSION     DASWVF010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456084"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1041
                     /locus_tag="VGD55_13990"
     CDS             133..1041
                     /locus_tag="VGD55_13990"
                     /inference="COORDINATES: protein motif:HMM:NF017831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HEY0871504.1"
                     /translation="MVDGRDDGHKGRTSTKYAAKKAATERIALARAAEQKAARRRNIM
                     VAGGSTLAVVVIIGLIVGIGLATKKSTGAGNPVNAASSTVTSGLASAAALTSQQPDLT
                     LVKGPPTRLTGAALTSGGKPEILYVGAEYCPYCAVTRWPLTVALSRFGTFSNLKTTLS
                     SATDAAGPNTPTLSYHGATYSSPYFNLVTREQEDGAGKPLETLSGAETSLFENYGKGA
                     YPFVDFGGTWMQSGASADQTELAGLTPDDVAKEIADPTTKPGAMTLAGADTFTAIICG
                     IDGGKPANVCTSVGVVAATAALSAIK"
     gene            1080..1559
                     /locus_tag="VGD55_13995"
     CDS             1080..1559
                     /locus_tag="VGD55_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0871505.1"
                     /translation="MRAGRQSGAPAWAAPVCLFLALVGIGLATYLTIAHYDTHVTLAC
                     SSKGAINCEAVTTSAESKLFGIPVAVLGLAYFIGMVPWHLPAAWRSPDPRIKIGRLLY
                     GLSGIGFVCYLIYAEAIVIKKICLWCTGVHITTFVLFIVTVFASALAIPTDPEDDEL"
     gene            complement(1759..>2837)
                     /locus_tag="VGD55_14000"
     CDS             complement(1759..>2837)
                     /locus_tag="VGD55_14000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0871506.1"
                     /translation="PVAAWQPPFVGTNNLVLHASDHIDDLGVLPMKTRYLGSGAHQLA
                     VSELGLGCMGMSEFYGATDDQESLTVIKHALDAGCVFLDTADMYGPFTNERLVGRAIA
                     GRRDEVVVATKFGNERSPDGTYVGVNGKPEYVRAACDASLERLGVDHIDLYYQHRVDK
                     SVPIEDTVGAMRELVEAGKVRHLGLSEASPSTIRRAHGVHPITALQTEYSLWSRDPED
                     ELLPMVRELGIGFVAYSPLGRGFLTGQIKTVDDFAENDFRRHGPRFQGENFQRNLDLV
                     QRVTEIAEAKGCTPGQLAIAWLLAQGDDIVPIPGTKRLGYLTENLGAVDVALTADDMA
                     AIEALAPKGSFAGDRYANMGSIDR"
BASE COUNT          502 a          921 c          922 g          492 t
ORIGIN      
        1 aaggaacctc gggcgtgctc cgatgcgttg ggcaaacagc gagcagttag gctgagcgca
       61 gcgaagccgc agtatgggtc gcttgggcaa agggcacgct tgatacggcg ctgatgtgac
      121 gaagggtgtc tgatggtcga cggacgcgac gacggtcata agggccggac gtcgacaaag
      181 tacgcggcca agaaggccgc gaccgaacgg atcgccctcg ccagggccgc cgaacagaag
      241 gccgcccgcc gacgcaacat catggtggca ggcggatcga cgctcgccgt ggtcgtcatc
      301 atcgggttga ttgttggcat cgggctcgcg acgaagaagt cgacgggcgc cggcaacccg
      361 gtcaacgccg cctcgagcac agtgacctca gggttggcga gcgcggcggc gctgacgtcg
      421 cagcagcccg atctcaccct ggtgaaaggt ccgcccacgc gactcaccgg cgcggcgctc
      481 acgagcggcg gcaagccgga gattctctac gtaggcgcgg agtactgccc gtactgcgct
      541 gtgacccgct ggccgctgac cgtcgcgttg agccgattcg ggaccttcag caacttgaag
      601 acgacgctgt cgtcggccac tgacgcggct gggcccaaca cgccgacgtt gtcgtatcac
      661 ggtgcgacct acagcagtcc gtacttcaac ctggtgaccc gcgagcagga ggacggcgcc
      721 gggaagccct tggagacatt gagcggcgcc gagacctcgc tgttcgagaa ctacggcaag
      781 ggcgcctacc cgttcgtcga cttcggcggc acctggatgc aaagcggggc cagcgccgat
      841 cagaccgagc tggcgggact cacgccagac gatgtcgcaa aggaaatcgc cgacccgact
      901 acgaagccag gcgcgatgac gttggccgga gccgacacgt tcaccgcgat catttgcggc
      961 atcgacggtg gcaagccggc caacgtctgc acgtctgtcg gcgtcgtcgc ggcgacggcc
     1021 gcgctcagcg ccatcaagta gcgacgccgg cccctaccgt tgtgctaacc ccaagtccgg
     1081 tgcgagcggg caggcaatcc ggcgcacccg cgtgggcggc gccggtctgc ctattcctcg
     1141 cgctggtcgg catcggtctc gccacgtatc tgacgattgc gcattacgac acgcacgtga
     1201 cgctggcgtg ctcgagcaag ggcgcgatca actgcgaagc cgtcaccacg agcgccgagt
     1261 cgaagctgtt cggcattccg gttgccgtgc tcggcctcgc atatttcatc ggaatggtgc
     1321 cgtggcacct gccggcggcg tggcggtcgc ccgacccgcg gatcaagatc ggccggctgc
     1381 tctacggctt atcgggcatc gggttcgtct gctacctgat ctacgccgag gcgatcgtca
     1441 tcaagaagat ctgcctgtgg tgcaccggcg tccacatcac gaccttcgtg cttttcatcg
     1501 tcacggtctt cgcgagcgcc ctggcgatcc cgactgaccc agaggacgac gaactctaga
     1561 ccggacgccg gccagtgtcc gtttcgtgtt tagtcgcccg attgtcgtca cattacgtga
     1621 cccggcgccg atgggttcgc aacgggtcgc ccgacaactg tcgcagtgat ccgccacgcg
     1681 gcgtccgaga gccctttgcc tgcccgcagg gtgatcggac gccgcacccc gcgctccacc
     1741 atcgcgcggg ccagctggct accggtcgat cgaacccatg ttggcgtaac ggtcaccggc
     1801 gaagctgccc ttgggtgcta acgcctcgat cgcggccatg tcgtcagctg tgagcgcgac
     1861 atcgaccgcg ccgagattct ctgtcaggta acccaaccgc ttggtgccgg gaatcgggac
     1921 gatgtcgtcg ccttgggcca gcaaccacgc gatggccagc tgccccggcg tgcagccctt
     1981 cgcttcggcg atctcggtca cgcgctgtac aaggtcgagg ttgcgttgga agttctcgcc
     2041 ttggaaccgc ggtccgtgcc gccggaagtc gttttcggcg aagtcgtcga cggtctttat
     2101 ctgacccgtc agaaagccgc ggccgagcgg gctatacgcg acgaaaccga tgccgagctc
     2161 acgcaccatg gggaggagct cgtcctcggg atcgcggctc cagaggctgt attccgtctg
     2221 caaagcagta atcgggtgga cgccatgtgc ccggcgaatt gtcgacggtg acgcctccga
     2281 caggccgagg tggcgaacct tgccggcttc caccagctcc ctcatcgcgc ccacggtgtc
     2341 ttcgatcggg acggacttgt cgacgcgatg ttggtagtac aagtcgatgt ggtcgacccc
     2401 aaggcgttcc aatgacgcgt cgcatgcagc gcgcacatat tcgggcttgc cgttgacccc
     2461 cacataggtg ccatcggggc tgcgctcgtt gccgaacttc gtcgcgacca cgacctcgtc
     2521 acgccggccc gcgatcgccc gacccacgag tcgctcgttg gtgaacgggc catacatgtc
     2581 ggccgtatcg aggaaaacgc acccagcgtc gagggcgtgc ttgataaccg tcagtgattc
     2641 ttggtcgtcc gtggcgccgt agaactcgct catgcccatg caaccgaggc cgagctcgga
     2701 aacagcgagt tgatgggcgc ccgaaccgag gtatcgggtc ttcatcggaa ggactcccag
     2761 atcgtctatg tggtctgaag cgtgcagcac cagattgtta gttcccacga aaggcggctg
     2821 ccacgctgcg acaggtg
//
LOCUS       DASWVF010000791         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_174976, whole genome shotgun sequence.
ACCESSION   DASWVF010000791 DASWVF010000000
VERSION     DASWVF010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174976"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..485)
                     /locus_tag="VGD55_14005"
     CDS             complement(33..485)
                     /locus_tag="VGD55_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871507.1"
                     /translation="MIAAPPFVVGALNATDAVVSPAVAATPVGADGDVLGVTALLGAD
                     ATPVPMPFIAVTVKVYAVPLVSPDTVIGELVPVAVKFPGADVTVYPLIGDPLAFGATN
                     DTAALATPAVALTPVGALGALGELVPDAAVTEQSSYTSDPFALVSFVE"
     gene            442..1134
                     /locus_tag="VGD55_14010"
     CDS             442..1134
                     /locus_tag="VGD55_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0871508.1"
                     /translation="MAFSAPTTNGGAAITSYTVVASPGTATGSGGASPISVSGLTNGT
                     SYTFTVTATNAAGTGPASGSSNGVTPVHTATTQLLPDPGFESGNGGWIAFKVGTLTRV
                     ATPVHGGAFALRVAATGTAATLVGLTQNSVVSTSVAGRSYTASCYVEPTSSASLDSTI
                     RLLEYTQSFSSDTQLQSVTVTNLHLNTWTLVQVTGAALRAGERIIPQIYSSKETTANG
                     SLVYDDCSLTGQ"
     gene            1351..>1584
                     /locus_tag="VGD55_14015"
     CDS             1351..>1584
                     /locus_tag="VGD55_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0871509.1"
                     /translation="MSDAWSFETKQIHAGQSPDEATNARALPIYQTTSYTFRDTDHAA
                     ALFGLAEFSNIYTRIMNPTQAVVEERVAALEGGV"
BASE COUNT          295 a          526 c          511 g          252 t
ORIGIN      
        1 gtgaactccg gcgagcggat gattccgcag atctactcga cgaatgagac gagtgcgaac
       61 gggtcgctcg tgtacgacga ttgttctgtc acggcagcgt ctggcacgag ttcaccgagt
      121 gctcccagtg cgccgaccgg cgtcagcgcg acggccgggg tcgcgagtgc tgcggtgtcg
      181 ttcgtcgccc cgaacgccaa cggatcgccg atcagtgggt acacggtcac atcagccccc
      241 gggaacttca ccgctactgg aaccagttca ccgatcacgg tgtctgggct gaccaacggc
      301 accgcgtaca ccttcactgt caccgcgatg aacggcatcg gcacgggtgt ggcgtcggcg
      361 ccgagcaatg cggtgacacc gaggacgtcc ccgtcggcac cgaccggagt cgccgcgacc
      421 gcaggcgaca ccactgcgtc agtggcgttc agtgccccca cgacaaacgg tggcgcggcg
      481 atcaccagct acaccgtggt cgcgagcccg ggcacggcga ccggtagcgg aggcgcgagc
      541 ccgatcagcg tgtcgggtct gacgaacggc acgagctaca cgttcacggt gacggcgaca
      601 aatgcggccg gcaccgggcc tgcgtcggga tcaagcaacg gcgtcacgcc cgtgcatacc
      661 gccacgacac agttgttgcc cgacccagga ttcgagtctg gaaacggcgg ctggatcgcg
      721 ttcaaggtcg gcacgctcac gagggtcgcc acaccggttc atggcggggc attcgccttg
      781 cgggtcgcgg cgaccggaac tgcggccacc ctggtcggcc tgactcaaaa cagcgttgtc
      841 agcacctcgg tcgccgggcg gtcgtacacg gcatcgtgct acgtagagcc gacgtcgtcg
      901 gccagcctcg actcgacgat caggttgctc gaatacaccc agagcttcag cagcgacacc
      961 cagctgcaga gcgtgaccgt cacgaacctg cacttgaaca cgtggacgct ggtgcaggtc
     1021 acgggtgcgg ccctgcgggc cggcgagcgg atcattccgc agatctactc aagcaaggag
     1081 accaccgcga acggcagcct ggtctacgac gactgttcgt tgacagggca gtagtccgct
     1141 cgacggaggg tccgcgagtg tggttgcatg gcggccatga tgcgaatgcc cttgttgctc
     1201 gaccccttct gggtcggccg ccgcgcgcat cactttgacc agcgctgtcg ctgaccagcg
     1261 cgcaatgcgc agccggcccg ctggtgggcc gtagcccctt cgtacttgtc cgatctatcc
     1321 ccatgcatgc caattcccga gaggattccc atgagcgacg cctggagttt cgagaccaaa
     1381 cagattcatg ccggccagag ccccgatgag gcgaccaacg cccgggcgct accgatctac
     1441 caaacgacct cgtacacgtt cagagacacc gaccacgcgg cggcgctctt cggacttgcg
     1501 gagttcagca acatctacac gcggatcatg aacccgacgc aggccgtcgt ggaggagcgc
     1561 gtcgcggcgc tcgaaggcgg tgtc
//
LOCUS       DASWVF010000792         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_201043, whole genome shotgun sequence.
ACCESSION   DASWVF010000792 DASWVF010000000
VERSION     DASWVF010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201043"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VGD55_14020"
     CDS             complement(<1..134)
                     /locus_tag="VGD55_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0871510.1"
                     /translation="MMAIGVLAFVVALLVSVMIHEAGHFVTAKLFGMKATQFFVGFGP
                     T"
     gene            complement(131..1354)
                     /gene="dxr"
                     /locus_tag="VGD55_14025"
     CDS             complement(131..1354)
                     /gene="dxr"
                     /locus_tag="VGD55_14025"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0871511.1"
                     /translation="MSQRRDVVILGSTGSIGTQALDVIRRNPELFRVVGLAAGGDRLD
                     LVADQAAEFNVEVVAIAHGSTSDLEAALAAASARAGVARASLKLIAGADAATEVAGWP
                     LVGTGVVLNGMTGSIGLAPTLAALHAGHTLALANKESLIVGGPLVKNAAAPGQIVPVD
                     SEHSALAQCLRGGQAGEVRRLILTASGGPFRGRPASDLATVTREQALAHPTWRMGPVV
                     TINSATLVNKGLEIIEAHLLFDLPLSAIDVVVHPQSVVHSMVEFTDGSTLAQCSPPDM
                     RLPIAMALAWPDRVPDAVPACDWTTAKTWTFEPLDDEAFPAVRLARHVGTTGGVAPAI
                     YNAANEEAVGAFLRGDLAFTGIVQTVSRVVDESAEEGMGNVGELVDVLRAETSARVRA
                     RSLIYGTDSPGELST"
     gene            complement(1351..3585)
                     /locus_tag="VGD55_14030"
     CDS             complement(1351..3585)
                     /locus_tag="VGD55_14030"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0871512.1"
                     /translation="MFERLGARVFNRRKRVLIVSLALLILGGLWGGSVSKHLSSGGYY
                     DPHSESVKAGNDINHKVDVPTVDAIVLFTATTPVTNPAVEQSIETALAALPHADVPSY
                     TTYWTSHQRQQLSTNTHATYVTLELTGKTDATQEASLAAVRDTLAKADLPGITVQYGG
                     TVTLNKEISGQVSKDLRSAELVSLPIVLVLLVIIFGSLVSAGLPLAVGILGIVGASAV
                     LRVLTTFVSVSVFALNLVTVLGLGLAVDYALFVVSRFREELDRQPTIERAVMRTMATA
                     GRTIAFSGITIAASLASLTLFPETFLRSMGYGGVAVVLVDVVAALTVLPALLAILGHR
                     VDSLRVPFGKSEDKSKRADAEQSGSALEESGWWYRLAHSVMRRPLAYTVAIVVLLGVM
                     AAPFANVIFGGYTSRVLPVSSPGRVVSDTLARDFPSNATNPIQVVVEGVSNPAVIAAY
                     VQKLSAVEGVTGASVAAAHGDNTLVQVGFNGDPISSGARSTLARVRAVPQPAGARILV
                     GGFTAFLVDQLRSIGHVLPWMLGTMIAAMLVLLFLAFGSFILPFKAVLMNALSIGASF
                     GAVTWVFQSGHLSNLFGFSKLGYLDATDPILMLAVVFGLSMDYEVFLLSRIREEWDAS
                     AASGEPPDRRNRVAVASGLQRSGRIITSAALLLVIVVGAFATSQVTTLKLLGFGMALA
                     IFVDATIVRAMLVPAVMRLLGGANWWAPAPLARWWVRHGLAESSDTDSDPSPGPGRYE
                     TLVR"
     gene            complement(3637..4284)
                     /locus_tag="VGD55_14035"
     CDS             complement(3637..4284)
                     /locus_tag="VGD55_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0871513.1"
                     /translation="MSMLMHLRMSVPDRPGMLAQITAALADVGADIRTIAVLERESGR
                     AIDDIYLSWPENRPASVLDQRVASVRGVTVLGLRPSRQVPGAFPDIDLLSQVLATASR
                     GLDTLVDMAALAFGADWAASVTYASGAPVVLYASARPDDVIVMPEIAVIRPAAIERDG
                     VQLAATPLSPFDAVLVLGRADKPSFHQAELERIRRVTELAVGLVRADVSVPATAR"
BASE COUNT          850 a         1525 c         1534 g          696 t
ORIGIN      
        1 gttgggccaa agcccacgaa gaactgggtg gccttcatcc cgaaaagctt tgcggtcacg
       61 aagtgaccgg cctcgtggat catgaccgaa accagcaggg cgaccacgaa ggccaggacg
      121 ccaatcgcca tcatgttgag agttctcctg gtgagtcggt gccgtagatg agggagcggg
      181 cccgcacgcg tgcagaagtc tccgcccgga gtacgtctac gagctcgccg acgttcccca
      241 tcccttcttc ggcagattca tcgacaaccc gagaaaccgt ctgaacgatc ccggtgaagg
      301 ccaggtcgcc gcgcaggaac gccccgaccg cctcctcgtt ggccgcgttg tagatcgccg
      361 gagcgacccc tccggtggtg ccgacatgcc gagccaggcg gacggcggga aacgcctcgt
      421 cgtccagcgg ctcgaaggtc caggtcttgg ccgtcgtcca atcacatgcg gggacggcgt
      481 cgggcacccg atctggccag gccagcgcca tcgcaatcgg gagccgcatg tcgggaggcg
      541 agcactgcgc cagggtcgac ccgtcggtga actcgaccat cgagtgcacg accgactggg
      601 gatgcacgac gacgtcgatc gcgctcagcg gcaggtcgaa gaggaggtgc gcctcgatga
      661 tctcgagccc cttgttgacc agcgtcgccg agttgatagt gacgaccgga cccatccgcc
      721 aggtcgggtg ggcgagggcc tgctcgcggg tgaccgtcgc cagatcggac gcgggccggc
      781 cgcggaacgg gccgccgctg gcggtcagga tcagccgccg cacttcacct gcctgcccgc
      841 ctcgaaggca ctgggcaagc gccgagtgtt ctgagtcgac gggcacgatc tggccgggtg
      901 cggccgcgtt cttcaccagc gggccgccca cgatcagcga ctccttgttc gccagcgcca
      961 aggtgtggcc ggcgtgcaac gcggcaagtg tgggcgcgag cccgatcgaa ccggtcatgc
     1021 cgttgagcac gacgccggtt ccaacgagtg gccagcccgc cacttcggtc gccgcgtcag
     1081 ccccggcgat gagcttgagc gacgcgcgag cgacgccggc ccgggctgat gcggccgcca
     1141 gtgcggcttc gaggtcggac gtcgagccgt gcgcgatcgc gacgacctcg acgttgaact
     1201 cggcagcctg atcggcgacc agatcgagcc gatctccgcc ggcagcgagt ccgaccacgc
     1261 ggaacaattc gggattgcgt cgaatgacgt cgagcgcctg ggtgccgatg gatccggtcg
     1321 accccaagat gacgacgtcc ctgcgctggc tcacctcacc agtgtctcgt accggccagg
     1381 tcccgggctc gggtcgctgt cggtgtcgct cgattcggcc aggccgtgac gaacccacca
     1441 tcgggcgagc ggcgccggtg cccaccagtt cgcgccgcca agcagtcgca tgaccgccgg
     1501 cacgagcatc gcgcgcacga tcgtggcgtc gacgaagatc gcgagcgcca tgccgaaccc
     1561 gagcagcttc aacgtggtga cctgcgacgt cgcgaacgcg ccgaccacga tgacaagcag
     1621 cagcgcggcg ctggtgatga tgcggccact gcgctggagt ccgctcgcaa ccgcaacgcg
     1681 attgcgacgg tcaggtggct cgccgctggc cgccgatgcg tcccattcct ctcggatgcg
     1741 actgagcagg aacacctcgt agtccatcga cagcccgaac acgaccgcca gcatcaggat
     1801 cggatcggtc gcatcgagat agccgagttt ggagaagccg aacaggttcg acaggtgccc
     1861 gctctggaac acccaggtga ccgcaccaaa cgaggcgccg atcgacaacg cgttcatcag
     1921 caccgccttg aacggcagga tgaacgagcc gaacgcgagg aacagcagca cgagcatggc
     1981 ggcgatcatc gtgccgagca tccacggcag cacgtgcccg atgcttcgca actggtcgac
     2041 cagaaacgct gtgaagccgc cgaccagaat gcgcgcgccc gcgggttgcg gcaccgcgcg
     2101 cacccgcgcc aacgtcgacc gcgcacccga cgagatcgga tccccgttga agcccacctg
     2161 caccagagtg ttgtcgccat gcgcggcggc aacggatgca ccggtcaccc cctcgaccgc
     2221 gctcagcttc tgcacgtagg cagcaattac ggccgggttc gagacacctt cgacgacaac
     2281 ctggatcggg ttcgtagcat tagaaggaaa atcgcgggcc aacgtgtcgc tcactactcg
     2341 accaggcgag ctgaccggca gcacccgcga tgtgtatccg ccgaagatga cgttcgcgaa
     2401 tggcgccgcc atcacaccga gcaatacgac gatcgcaacg gtgtacgcga gtggccggcg
     2461 catcacactg tgggcgaggc ggtaccacca accgctttcc tcgagggcgg accccgactg
     2521 ctcggcgtcc gccctcttgc tcttgtcctc gctctttccg aaaggcactc gcagtgagtc
     2581 gactcgatgg cccaaaatcg ctagcagggc tggcaaaacc gtcaatgcgg cgacgacgtc
     2641 tacgagcacg acggcgactc ccccgtagcc catcgaccgc aagaacgtct cgggaaagag
     2701 cgtcagcgac gcgagtgatg cggcgatcgt gatgccggag aaggcgatcg tgcgaccggc
     2761 cgttgccatc gtgcgcatga cagcgcgctc gatcgtgggc tggcggtcga gctcctcgcg
     2821 gaaccgactc acgacgaaga gcgcatagtc gaccgcaagg ccgaggccca gcacggtcac
     2881 caggttcagc gcgaagaccg acacgctcac gaatgtggtg agcacgcgca agaccgctga
     2941 cgcgccgaca atgccgagga tcccgacggc cagcggcagg cccgcagaaa cgaggctgcc
     3001 gaagatgatg acgagcagga cgagcacgat cggcaacgag acgagttcgg cactgcgcag
     3061 gtctttgctg acctgcccgc tgatttcctt gttcagggtg accgtgccgc cgtactgcac
     3121 cgtgatgcca ggcaggtcgg cctttgcgag ggtgtcgcgg actgctgcga gtgacgcctc
     3181 ctgcgtcgca tcggtcttgc cggtcagttc gagggtgacg taagtggcat gggtgttcgt
     3241 cgagagttgc tggcgttgat ggctggtcca gtaggtcgtg tagctcggca cgtcggcatg
     3301 cggcaaggca gctagggcgg tctcgatcga ctgctcgacc gcggggttgg tcacgggcgt
     3361 ggtcgcggtg aacaacacga tcgcgtcaac ggtcgggacg tcgaccttgt ggttgatgtc
     3421 gttgccggcc ttgaccgact cgctgtgcgg gtcgtagtac ccgccgctcg agagatgctt
     3481 gctgaccgag ccaccccaaa gcccaccgag aatcagcagg gccagggaga cgatcagaac
     3541 acgcttgcgc cggttgaaca cccgcgcccc aagacgctcg aacataccga tcaggctaaa
     3601 ggtcgagcag ctggtcctac cacgccgcct gcactactac cgtgcggtcg ctggcaccga
     3661 gacgtcggcc cgcacaagac ccaccgcaag ctcggtgacc cgccggatcc gctccagctc
     3721 cgcctggtgg aacgacggct tgtcggcccg cccgagcacc aacacagcat caaacggcga
     3781 cagtggggtc gctgccagct gaacgccgtc ccgctcgatg gcggctgggc ggatcactgc
     3841 gatctcgggc atcacgatga cgtcgtcggg gcgggcgctc gcgtagagca ccacaggagc
     3901 gcccgacgcg tacgtgacgc tggcggccca gtcggcgccg aaggccagcg ccgccatgtc
     3961 gaccagagtg tcgaggccgc gcgaggcggt ggcgagaacc tgactgagca ggtcgatgtc
     4021 ggggaacgcg ccgggcacct ggcgcgacgg gcgcaagccg agcacggtga cgccgcgcac
     4081 actcgcgacc cgttggtcga gcacgctggc cggccgattt tcgggccagc tcaggtagat
     4141 gtcgtcgatg gcccggccgg attcacgttc gagaacggcg atggttctga tgtcggcgcc
     4201 gacgtctgcg agggcggcgg tgatctgcgc aagcatcccg ggacggtcgg gcacgctcat
     4261 ccgcaggtgc atcagcatcg acatctccga tcgtcggtct ggcacttaca ttcctggcag
     4321 tcccgccgcg ctcccaagca tgccagggaa aggtttccgg cgagtgacgt gcgggtgacg
     4381 cgctagtcac ctgcgtgctg acggccgggt tcggagagcg gtccggccgg cgtcggatct
     4441 gcgacgccgg ccgggccaaa tacgccaacc cgacgacgga ttccgtcgcc gaagtcgtta
     4501 ccgacggtcg ttaccatcag gttccacgag ggcgttggtc ggatacagta catgtgcctg
     4561 atcgtgtgcc accaattgcc gggagacgac cctcatgacc gatca
//
LOCUS       DASWVF010000793         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_72927, whole genome shotgun sequence.
ACCESSION   DASWVF010000793 DASWVF010000000
VERSION     DASWVF010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72927"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGD55_14040"
     CDS             complement(<1..204)
                     /locus_tag="VGD55_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871514.1"
                     /translation="MRWSDVRRADIERVKALSERRLTPEEWDAYVNQPVTEDEERDMR
                     ELLSWFSRRYPTPADRLRYARRAY"
     gene            210..659
                     /locus_tag="VGD55_14045"
     CDS             210..659
                     /locus_tag="VGD55_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871515.1"
                     /translation="MFSAVAAQRRKKPEVFRVFWPGRETLGRFEVVDAGNGHDYAGGH
                     FDVLTPRPYIVERDSPAVHRWNGAGDQNVDVLAIFEDVPPASAGLCGRVSRNEAQRLH
                     GGVEQFDVVEMYPEVVIRCQDGDSQGDQRATPDENGARARIGDAAKK"
     gene            complement(745..1767)
                     /locus_tag="VGD55_14050"
     CDS             complement(745..1767)
                     /locus_tag="VGD55_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871516.1"
                     /translation="MNITSRLLACALLPIAGVGCGSSGGSSGTGAAATCTNGMIKAAE
                     GNDYVFSSTITLPPVTVKSMANLTIDWSVVNHDFLGHTLAQATDTGLALIMLWKEPLA
                     EFQMNLNADNLLASDLIVSPPPSIERSAGKTSAMLYDFTINGTPVSSSDFNAYFDPSK
                     YSPASNTYLAAIQTGTNLGTGIRMLQAFQLDPASSNTTVTLTNSSTKLQYSTNLHSLQ
                     PTGVPASTPRLTLDWGDMKKNALGADFITTNITSAIVGHYTQTPSQLESQFLDLQTIA
                     TDLYTADIPSGTVLDFTTLKDGKGSAFPGVDATGTWLVALTCGNCRNPAPWYLTILEP
                     VAQPCK"
     gene            complement(1788..>2265)
                     /locus_tag="VGD55_14055"
     CDS             complement(1788..>2265)
                     /locus_tag="VGD55_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEY0871517.1"
                     /translation="ITKLTKTGSTVWVLNGGVGGTTNTFTGDTWAGGQHGIDVLGMND
                     FLIFNNNSSSGANPAGGGSAGSGKGSVAIEMKVDTGTKKATKAWTYKSSAGIQVDIMG
                     DLQRLPNGNTVVGYSTKGELQEIDASGTVLQDIKWPAGTSFGYIEKRQTLYGKPTR"
BASE COUNT          366 a          691 c          759 g          449 t
ORIGIN      
        1 atacgcccgg cgcgcataac gcaggcgatc tgcaggcgtc gggtaccggc gcgagaacca
       61 cgagaggagc tcgcgcatgt cgcgttcctc gtcctcggtc actggctggt tgacgtaggc
      121 atcccattct tccggcgtga gccgccgctc gctgagcgcc ttcacccgct cgatgtcggc
      181 gcgtcgtaca tcggaccaac gcacgactaa tgttctccgc ggtcgcggcg cagcgccgca
      241 agaagccgga ggtcttccgc gtcttttggc cgggccgcga aacgcttggt cgcttcgagg
      301 tcgtcgatgc agggaacggc cacgactacg ccgggggcca cttcgatgtt ctcacgccgc
      361 gcccatacat cgtcgaacgc gactcgccgg ccgtccaccg ttggaacgga gcgggcgacc
      421 aaaacgtcga cgtgctcgcc attttcgagg acgtaccgcc cgcgtccgcg ggcctctgcg
      481 gcagagtgag tcgcaacgag gcccagcgac tccacggcgg cgttgaacaa ttcgatgtcg
      541 tcgagatgta tccagaagtc gtaatccgct gtcaggacgg ggattcccag ggcgatcagc
      601 gcgcgacgcc cgatgagaac ggcgcgcgcg ccagaattgg tgacgctgcg aaaaaatgag
      661 gcttcgtcga aatcgtcgtc ctccaccccg aagagtgtac gacaacgctc gttgggccga
      721 caggagaggc cgcggcccct gtggtcactt gcagggctgt gcgacaggct cgaggatcgt
      781 caggtaccag ggggcggggt tgcggcagtt gccgcaggtg agggcgacca gccacgtgcc
      841 ggtcgcgtcg acgccgggga aggcgctgcc ctttccgtcc ttcagcgtcg tgaagtccag
      901 gaccgttccc gacgggatgt ccgccgtgta aaggtccgtg gcgatggtct gcaagtccag
      961 gaactggctc tcgagctgcg acggcgtctg cgtgtagtgg ccgacgatcg cgctggtgat
     1021 gttggtcgtg atgaagtcgg cccccagtgc gttcttcttc atgtcacccc agtccagcgt
     1081 caacctcggc gtgctcgcgg ggacgcccgt cggttgcaga ctgtgcaggt tcgtcgagta
     1141 ctgcagcttc gtcgatgagt tcgtgagcgt cacggtcgtg ttggacgacg cagggtcgag
     1201 ctgaaacgcc tgcagcatcc ggattccggt acccaggttg gtccccgtct ggatcgcggc
     1261 cagataggtg ttgctggcgg gggaatactt cgacgggtcg aagtacgcgt tgaagtcgct
     1321 ggagctgact ggcgtgccgt tgatcgtgaa gtcgtagagc atggcggagg tcttaccggc
     1381 ggagcgctcg atactgggag gcggcgagac gatcagatcg gaggccagca ggttgtcggc
     1441 gttcaagttc atctggaact ccgccagcgg ctctttccag agcatgatca gcgccagacc
     1501 ggtgtcggtc gcctgcgcca gcgtgtgccc gaggaagtcg tggttgacga cgctccagtc
     1561 gatcgtgagg ttcgccatcg acttcacggt gacgggcggc agcgtgatcg tgctcgagaa
     1621 gacgtaatcg ttgccttcgg ccgccttgat catgccgttc gtgcacgtcg ccgcggcgcc
     1681 ggtgcccgag gagccgccgc tgctgccgca accaacgccg gcgatcggca acaaagcgca
     1741 cgcgagaaga cgggacgtga tgttcattcg atcctttacg tatcggctca acgggttggc
     1801 tttccataaa gcgtctggcg tttttcgatg tagccaaacg aggttccggc cggccatttg
     1861 atgtcctgca aaacggtgcc gctggcatcg atctcttgca gctcgccctt cgtcgagtag
     1921 ccgacgaccg tgttgccgtt gggcagccgc tggaggtcac ccatgatgtc gacctgaatt
     1981 cccgcgctgg acttgtacgt ccacgccttg gtcgccttct tggtgccggt gtcgaccttc
     2041 atctcgatgg cgaccgagcc tttgcctgac ccggcgctgc cgcccccggc ggggttcgcg
     2101 ccgctggagc tgttgttgtt gaagatcagg aagtcgttca ttcccaggac gtcgattccg
     2161 tgctgacccc cggcccacgt gtcgccggtg aacgtgttgg tggtgccgcc gacgccgccg
     2221 ttgagcaccc agaccgtgct gccggtcttc gtgagtttgg tgatg
//
LOCUS       DASWVF010000794         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_405024, whole genome shotgun sequence.
ACCESSION   DASWVF010000794 DASWVF010000000
VERSION     DASWVF010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405024"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VGD55_14060"
     CDS             complement(<1..880)
                     /locus_tag="VGD55_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY0871518.1"
                     /translation="MRVRRLRRLSRASLVAAVMAAAVGVPAASVLPAAAASAGPALTV
                     DAAAGRHPISSDVYGMNFSDTALATELGLTADRWGGNSTSRYNYTNNTHNTGSDYYFE
                     NVVDSTPLDSFVAADLAHGDNPVVTVPAMGWVSKNSPSTHPFACGFKVSIYGAQQSTD
                     PYDATCGNGKHTNGTAITGNSPSDTSVTEDGAFDQAMVAHLVSKFGTAASGGVRSYEI
                     DNEPSLWSSTHVDVHPAPLSYNELWTKSLAAAQGIKAADPTATVDGPGDWGWCAYFYA
                     PDDPGGCGPGPDKTAHG"
     gene            1006..2028
                     /locus_tag="VGD55_14065"
     CDS             1006..2028
                     /locus_tag="VGD55_14065"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0871519.1"
                     /translation="MKVAMLAPIAWRTPPVHYGPWELVTSLLTEGLVARGVDVTLFAT
                     LDSQTTADLRGISPHGYAEEPSMDGRIWEAMHVANALAESKNFDLVHNQLDWLPLAFA
                     AHCAAPMVTTIHGFSGAAILPAYKRARSNYVAISDADRSPELDYIATIYHGVDLTALP
                     LNAAPGDDLVVFGRIHPDKGTAQAITIAREAGRRLVICGIVQDDHYFAEQIEPAVDGD
                     RVRYLGSIGPQQRAEVLGSAVALLHPIAFDEPFGLSVVEAMACGTPVIAYRRGSMPEV
                     VDEGVTGYLVDDAAGAVAAVERAAQLNRAAVSARAAARFGVDRMVDDYVRTYEQILSA
                     ASGSSR"
     gene            complement(2000..>2655)
                     /locus_tag="VGD55_14070"
     CDS             complement(2000..>2655)
                     /locus_tag="VGD55_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871520.1"
                     /translation="GMEDARFVRFLADDGTTDYRATYTAFDGHKIAPRLLTSPDLRRF
                     RAHRLAGPAARNKGMALFPRLVGGRHRSLCRSDGESTSLATSSDGFLWGAPELIQAPE
                     ASWEVLQVGNCGPPIETDRGWLVLTHGVGPMRTYSIGALLLDIDNPAHVVARLERPFL
                     QPTAQEQNGYVPNVVYSCGGMVHDGLLWLPYGIGDARIGVAWASVDELLSEMTPTRH"
BASE COUNT          442 a          891 c          865 g          457 t
ORIGIN      
        1 ttccgtgtgc cgtcttgtcg ggcccgggtc cgcagccacc gggatcatca ggtgcgtaga
       61 agtacgcgca ccagccccaa tcgccgggcc cgtcgacggt cgccgtcggg tctgcggcct
      121 tgatgccctg ggcagcggcg agcgactttg tccacaactc gttgtagctc agcggcgcag
      181 gatgcacatc gacgtgggtg ctgctccaca gcgacggctc gttgtcgatc tcgtaactgc
      241 gaacaccgcc ggaggcagcg gtgccgaact tcgacacgag atgcgccacc atcgcctgat
      301 cgaacgcgcc gtcttcggtc accgatgtgt cggacggtga gttgccggtg atcgccgtcc
      361 cgttggtgtg cttgccgttc ccgcaggtcg cgtcgtacgg atcggtcgac tgctgcgcac
      421 cgtagatcga gaccttgaag ccgcaggcga acggatgtgt cgacggcgag ttcttcgaca
      481 cccagcccat cgccggaacc gtgacgacgg ggttgtcgcc gtgcgcgaga tctgccgcca
      541 cgaaagagtc gagtggcgtc gagtcgacaa cgttctcgaa gtaatagtcg ctgcccgtgt
      601 tgtgggtgtt gttcgtgtag ttgtagcggc tggtgctgtt gccaccccag cggtctgccg
      661 tcagaccgag ctcggttgcc agcgccgtgt cactgaagtt catgccgtag acatcgctgc
      721 tgatcgggtg ccggccagct gcagcgtcga cggtgagcgc ggggccggca gaggccgcgg
      781 cggccggcaa tacggaggcg gccggcacgc cgaccgcggc agccatcacc gcagccacca
      841 ggctggctcg agacagtcgt cgtaacctgc gcacgcgcat gacgacgacc ttaggccccc
      901 ggcgtcccgg attaggtcac ttccgcccga ttttccccct atcggcgcaa gcccgccgct
      961 ggtcggcaag aatgcggtga tgagcatgcg gtcggtcgcg cgcgcgtgaa ggtcgccatg
     1021 ctggcgccga ttgcctggcg aacgccgccg gtgcactacg gtccgtggga actcgtgacg
     1081 agcctgctca ccgagggcct cgtcgcacgc ggcgtcgatg tgacgttgtt cgcgacgctg
     1141 gattcgcaga cgaccgcaga cctgcggggc atcagcccgc atggttacgc cgaagaaccg
     1201 tcgatggacg gccgcatctg ggaagcgatg cacgtagcga acgcattggc cgaatcgaag
     1261 aacttcgatc tcgtgcacaa ccaactcgac tggctgccgc tcgccttcgc ggcccactgt
     1321 gcggcaccga tggtcaccac cattcacgga ttctccggcg ccgcaatcct gcctgcgtac
     1381 aaacgggccc gctcgaacta tgtcgcgatc tccgacgccg accgctcccc cgagctcgac
     1441 tacatcgcga cgatctatca cggcgtcgac ctcaccgcgt tgccactgaa cgccgcgccc
     1501 ggcgatgacc tcgtcgtgtt cggccgcatc cacccagaca agggcaccgc gcaggccatc
     1561 acgatcgcgc gcgaagccgg tcggcggctg gtgatctgcg gcatcgtcca ggacgatcac
     1621 tacttcgcag agcaaataga gcctgcggtg gacggcgatc gggtgcgcta tctgggctcg
     1681 atcggtccgc agcagcgcgc tgaagtgctc ggctcggcgg ttgccttact tcatccgatc
     1741 gcgttcgacg agccgttcgg gctctcggtt gtcgaggcga tggcgtgcgg aacgccagtg
     1801 atcgcctacc ggcgcgggtc gatgcccgag gttgtcgatg agggcgtcac cggctatctc
     1861 gtcgacgacg ccgcaggtgc cgtcgccgcc gtcgagcgag ccgcccagtt gaatcgggcg
     1921 gccgtgagtg cacgtgccgc agcgcgcttc ggagtcgatc gcatggtcga cgactacgtg
     1981 cgcacctatg agcagatcct cagtgccgcg tcggggtcat ctcgctgagc aactcgtcga
     2041 ccgaggccca cgcaacgccg atgcgcgcat caccgatgcc gtacgggagc cacagcaggc
     2101 cgtcgtggac catgccacca cacgaataca ccacgttcgg cacgtagccg ttctgctcct
     2161 gtgccgtcgg ctggaggaac gggcgttcga ggcgagcgac cacgtgcgca gggttgtcga
     2221 tgtcgagcag caacgcaccg atcgaatatg tgcgcatcgg cccgacaccg tgcgtgagca
     2281 cgagccaccc tcggtcggtc tcgatgggcg gcccgcagtt gccgacctga agcacctccc
     2341 acgacgcctc cggcgcctgg atcagttccg gggcacccca aagaaaccca tcgctggacg
     2401 tcgcgaggct ggtgctctcg ccatctgatc gacagagcga acgatgacga cccccgacga
     2461 gcctcggaaa cagcgccatg cccttattgc gcgcggcggg cccggctagc cggtgcgcgc
     2521 ggaatctgcg cagatcaggg ctcgtcaaca atcgaggcgc gatcttgtgc ccgtcgaacg
     2581 cggtgtacgt cgcgcgatag tcggtcgttc cgtcgtccgc caagaatcgc acgaaccgcg
     2641 catcttccat cccgt
//
LOCUS       DASWVF010000795         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_22798, whole genome shotgun sequence.
ACCESSION   DASWVF010000795 DASWVF010000000
VERSION     DASWVF010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22798"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGD55_14075"
     CDS             complement(<1..425)
                     /locus_tag="VGD55_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871521.1"
                     /translation="MADNLSSSTDAARSDGPRTDHRPWTGYFVAFCGIDGSGKTTQVA
                     AAHEYLATTADVFLTKQPTDLYRNDPDVRALLDMRTGGALLTAELALLSAFDRARHVR
                     TQILPALAGDGCVITDRYVYSTYSYFMARGVNDLAWLKD"
     gene            complement(418..1272)
                     /gene="pyrF"
                     /locus_tag="VGD55_14080"
     CDS             complement(418..1272)
                     /gene="pyrF"
                     /locus_tag="VGD55_14080"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0871522.1"
                     /translation="MPPLPETFGDRLAVALRERGPLCVGIDPSAELLAAWGMPNSAAG
                     LAEFGRRVLDAVVDLVAVVKPQAAFFERLGSGGIASLEELCRDARARGLMVLLDAKRG
                     DIGSTSEAYAQAYLQADSPLEIDAMTATPYLGVASLAPMFDAARASGRGVFVVARSSN
                     PEGVRIQDAIVVGNGDVVSHVLDEVGAVNRLAPGNVLGSIGVVFGATCPTEGYDLKAF
                     GGPILAPGLGAQGASVDDIARRFAGYQDAVLPTVSRGVLGAGPDVAAMRAATVELVDD
                     CRKALARG"
BASE COUNT          294 a          534 c          486 g          228 t
ORIGIN      
        1 atgtccttca accacgcaag gtcgttcacg ccgcgcgcca tgaagtagct gtaggtcgaa
       61 tagacgtacc tgtcggtgat cacgcagccg tcgcctgcga gcgcagggag gatctgggta
      121 cggacgtgac gcgcgcggtc gaacgccgac agcaacgcga gctcggctgt gagcaacgcc
      181 ccgcccgtgc gcatgtcgag taacgccctg acgtcggggt cgttgcggta caggtcggtc
      241 ggctgcttgg tcaagaacac gtcggcggtc gtcgcgaggt actcgtgcgc tgcggcgacc
      301 tgggtcgtct tcccgctccc atcgatgccg cagaacgcga cgaagtagcc agtccatgga
      361 cggtggtcgg tccgcggtcc gtcggaacgt gccgcatcgg tgctcgatga gaggttgtca
      421 gccacgagcg agtgcctttc tacaatcatc gacgagctcg accgtggcgg cacgcatcgc
      481 ggcaacgtcg ggcccagcac ctagcactcc ccgcgaaacc gtgggcaaga ctgcgtcctg
      541 gtaaccggcg aagcgccgcg cgatgtcatc gacgctcgca ccctgcgcgc caagaccggg
      601 cgcaagaatc gggccgccga aggccttcag gtcgtagccc tccgtcgggc aggtggcccc
      661 gaacaccacg ccgatcgagc caagaacatt tcccggagcg agccggttga cggcgccgac
      721 ctcgtcgagc acgtggctca cgacgtcacc gttgcccacg acgatcgcgt cctgaatgcg
      781 gacgccctcg gggttggaac tgcgagcaac cacgaacaca ccccggccgc tcgcccgagc
      841 ggcatcgaac atcggagcaa gcgatgccac gcccagatag ggcgtcgcag tcatcgcatc
      901 gatctcgagt gggctgtctg cttggaggta ggcctgcgca tacgcctccg acgtcgaacc
      961 gatgtcaccg cgcttcgcgt cgagcaagac catgagaccg cgcgcccggg cgtcgcgaca
     1021 tagctcctca aggcttgcga tcccgccact accgaggcgc tcgaagaagg ccgcctgcgg
     1081 tttgacgact gccaccaggt cgacgaccgc gtcgagcacg cgacggccga actccgcgag
     1141 cccggccgcg ctgttaggca tgccccacgc cgccagcagc tccgcgctcg ggtcgatccc
     1201 gacacacagc gggccgcgct cgcgcaacgc aacagccaga cggtcaccga acgtttccgg
     1261 caacggtggc accagcaccg accccccacc cctcaagctt tcgccgccgg gcagtcaccg
     1321 cctgggacgc aaaaaagacg caaaaagaaa tgcgtaagcg ccgtcctaag gacttcgatg
     1381 gcgcccccgc accacttcag tccgacgcga atgccgcgtg acgactcgcg tcgagagtat
     1441 gtgttcaccc gcacgcgcgg cggtgattgg taaaaatttc aaccgaacgg ctgagcgcga
     1501 gacgttacat cgcgcccgag tttgttatgg cgcgaggccg cc
//
LOCUS       DASWVF010000796         5039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_327903, whole genome shotgun sequence.
ACCESSION   DASWVF010000796 DASWVF010000000
VERSION     DASWVF010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327903"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGD55_14085"
     CDS             <1..421
                     /locus_tag="VGD55_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0871523.1"
                     /translation="WSDRCQREGVEFSLEAPDWAVFVDTDPTRVRQVIDGLAENALRV
                     TPAGRPIVFFVGASGSDAILQVRDGGPGLTEDDCKVAFDRSALYDRYRGVRRVGTGVG
                     LALVAGLTARLGGRAAAGRSPEGGACFTITLPLRPGG"
     gene            511..1317
                     /gene="trpC"
                     /locus_tag="VGD55_14090"
     CDS             511..1317
                     /gene="trpC"
                     /locus_tag="VGD55_14090"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY0871524.1"
                     /translation="MSVLDEILVGVRADMEQRQRTMPLDDLKVKAAKRDAIVDGVAAL
                     KRDAVTVIAEVKRSSPSRGEIATIADPAALACAYEAGGAAVISVLTEQRKFHGSLADL
                     EAVRKAVDVPLLRKDFIYHSYQLWEALAYGADLVLLIVAALDQSALVSLVERAQSLGL
                     TPLVEVHDEAEVDRAIEAGARVIGVNARDLHTLEVDRSLFARLAPRIPPGIVKVAESG
                     VRGPHDLFAYASAGADAVLVGEGLVTDKDPRQAVADLVTAGAHPALRHGQ"
     gene            1355..2614
                     /gene="trpB"
                     /locus_tag="VGD55_14095"
     CDS             1355..2614
                     /gene="trpB"
                     /locus_tag="VGD55_14095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0871525.1"
                     /translation="MIAPKLPDSGGHFGPFGGRFVPEALVAALDQLSAAYTDAIADPA
                     FTNELTRLLTTYAGRPTPITDATRLSEYAGGARILLKREDLAHTGSHKINNVLGQALL
                     TVRMGKKRVIAETGAGQHGVATATAAALLGLQCTIYMGEEDTRRQALNVVRMKLLGAE
                     VIPVTAGTRTLKDAINEAFRDWVTTVDDTHYLFGTVAGPHPFPALVRDFQKVVGEEAR
                     AQVLALTGRLPDAVTACVGGGSNAIGIFTAFLSDNAVRLYGFEAGGDGVASGRHAATL
                     SAGSRGVLHGARTYVLQDSDGQTLASHSISAGLDYPGVGPEHAWLAETGRVTYQPVTD
                     SEAMDAFLLLARTEGIIPAIESAHALAGTLHLGRQLGPDAVVLVSLSGRGDKDVDTAA
                     KWFDLESASGSASGSASGSAAPVAALR"
     gene            2611..3486
                     /gene="trpA"
                     /locus_tag="VGD55_14100"
     CDS             2611..3486
                     /gene="trpA"
                     /locus_tag="VGD55_14100"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0871526.1"
                     /translation="MTVLPTEQSEAAVTSAAPAAVPVTAVPSRLSATLARCKAEGRAA
                     LIGYLPACFPNPERGIAALLALVEGGVDAVEIGLPYSDPVLEGPVIARAHDVALRAGS
                     TTDRVLRTVEAVAATGVATVVMTYWNPIEQYGVDRFAAALAAAGASGLVTPDLLPDEA
                     EPWIAASDAHGLDRIFLVAPSSTDSRIALTVRHCRGFIYAAAVMGVTGTRAQTSSLAP
                     PLVDRIRAITSLPVGVGLGVSNADQAAEIAGYADAVIVGSAFVRRVLEARGPQDEAVA
                     VRSLATELAAGVRRG"
     gene            3526..4554
                     /gene="lgt"
                     /locus_tag="VGD55_14105"
     CDS             3526..4554
                     /gene="lgt"
                     /locus_tag="VGD55_14105"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0871527.1"
                     /translation="MIQLAVLPAYIPSPSDGVWHLGPIPIRGYALCIIVGIFAAAVLA
                     DRRYRDRGGAIGVINEVSTWAVPFGLVGGRIYHVLTDWSDYFGKGGSPIRSLEVWKGG
                     LGIPGAVALGGLGVLVACRRRGVLMPPIADAIAPGLVLAQGIGRWGNWFNQELYGRPS
                     GLPWAVRIDTNHQVGVILTKYQESPYGFYQPTFLYESIWDISTAVILILLDRRYKLGH
                     GRVFALYLVIYGLGRGWIEALRIDPAHHFFGLRLNDWTSIIIVVAGVVGYIVSARRHP
                     GREVDLVRAPVAALPTEPAAAEADAAAAADDAAADDAAAEVTVGSDADHDAPPGASSG
                     GSHEGAGT"
     gene            4551..>5039
                     /locus_tag="VGD55_14110"
     CDS             4551..>5039
                     /locus_tag="VGD55_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY0871528.1"
                     /translation="MTTADETEAAQPVKPTAEIHHDHRDVTGGWLRPAVFGAMDGLIS
                     NFSLISGFSGANASRHLLILSGLGGLVAGAFSMAVGEYVSVASQGDLARAEIEVERHE
                     LRHNAAAEQAELADLYVSRGVDRALALEVSKQLSRDPDQALAIHAREELGISADALPS
                     PYL"
BASE COUNT          770 a         1713 c         1686 g          870 t
ORIGIN      
        1 gtggtcggat cgctgccaga gggaaggcgt cgagttcagc cttgaagccc ccgactgggc
       61 ggtctttgtc gacaccgatc cgacccgagt gcggcaggtc atcgacggtc tcgctgagaa
      121 cgcgttacgc gtcacaccgg ccgggcgacc gatcgtgttc ttcgtcggcg cgtccgggtc
      181 cgacgcgatt ctgcaggtgc gcgatggcgg ccccggactt actgaggatg actgcaaggt
      241 tgcctttgac cggtccgcgt tgtacgaccg gtatcgcggg gtccgccggg tcggaacggg
      301 ggtcggactc gcactggtgg ccggtctcac ggcgcggctc ggcggtcgcg cggccgccgg
      361 caggtcgccg gaaggtggcg cctgcttcac gatcaccttg ccgctgcgcc ctgggggctg
      421 acggccgggc tgatttcgct ggccgctacc ctgaggcagt tccagaagtg ctcgacagcc
      481 ggctaacagg gcgcacgagg ggatcgctag gtgagcgtgc tcgacgagat cctggtcggc
      541 gtccgcgccg acatggagca acgccagcgc acgatgccgc tcgacgacct caaggtcaag
      601 gctgcgaagc gcgacgcgat cgttgacggc gtggccgcat tgaagcgcga cgcggtcact
      661 gtcatagccg aggtgaagcg gtcgagtcct tcgcggggtg agatcgcgac catcgctgac
      721 ccggcggcac tagcgtgtgc gtacgaagcc ggcggcgccg cagtgatcag cgtgctgacc
      781 gagcagcgca agttccacgg ctctctcgcc gacctcgaag cggtgcgcaa ggcggtagac
      841 gttcccttgc tgcgtaaaga cttcatctac cattcgtatc aattgtggga agcgctcgcg
      901 tacggtgccg accttgtatt gctcatcgtc gcggcgctcg accaaagcgc actcgtgtcg
      961 cttgtcgaac gcgcgcaatc ccttgggctg accccgctgg tcgaggtgca cgacgaggcc
     1021 gaggtcgacc gcgccatcga ggcgggtgcg cgcgtgatcg gcgtcaatgc gcgcgacctg
     1081 cacaccctcg aggtcgacag gtcgttgttt gcgcgacttg ctccgcgcat cccccctggc
     1141 atcgtgaagg tcgccgagtc aggggtgcgc ggcccgcacg acctgttcgc gtacgcctcc
     1201 gccggagccg acgcggtcct cgtcggtgag ggtctggtga ccgacaagga tccgcgacag
     1261 gccgttgccg atctcgtcac ggctggcgcg catcctgcgt tgcggcacgg acaatagtgc
     1321 cggtatgtct gctgccgtcg tctaggcttg atcggtgatc gcccccaagc ttccagactc
     1381 aggtggccac ttcggccctt tcggtggtcg gttcgttccc gaagctctcg tcgctgcact
     1441 cgaccaactc agcgccgcat acaccgacgc gatcgccgac cctgccttca ccaacgagtt
     1501 gacgcgactg ctcacgacgt atgcggggcg acccaccccg atcactgatg cgacgcggct
     1561 gtccgaatat gcgggtggtg cgcggatcct cttgaagcgc gaagacctcg cgcataccgg
     1621 ctcgcacaag atcaacaacg ttctcggcca ggcactgctc accgtgcgca tgggcaagaa
     1681 gcgagtgatc gccgaaaccg gggcgggcca gcacggcgtt gccaccgcaa cggctgcggc
     1741 gctgctcggg cttcaatgca cgatctacat gggcgaagaa gacacccgcc ggcaggcttt
     1801 gaacgtcgtc cgcatgaaat tgctcggcgc cgaagtgatc cccgtcaccg ccggcacccg
     1861 cacgctgaaa gatgcgatca acgaagcctt ccgcgactgg gtcacgaccg ttgacgacac
     1921 ccattacctg ttcggcaccg tcgccgggcc gcatccgttc cccgcactgg tccgtgattt
     1981 ccagaaggtc gtcggtgagg aagcacgagc gcaggtgctc gcgctgaccg gtcggcttcc
     2041 cgatgcggtc accgcctgcg tgggcggcgg gtcgaacgca atcggcatct tcacggcatt
     2101 cctgtccgac aacgcggtgc gcctgtacgg attcgaagcc ggtggcgacg gagttgcctc
     2161 tggccggcac gcggcgacgt tgagcgcggg cagccgcggc gttctgcacg gcgcgcgcac
     2221 ctacgtgctt caggactccg acgggcagac cctggcgtcg cactcgatct cggccggtct
     2281 cgactaccca ggtgttggcc ccgagcatgc ctggctcgcc gagacgggcc gcgtcaccta
     2341 ccagccggtc accgactcag aggcgatgga cgcgttcctg ctgctcgctc gcaccgaagg
     2401 catcatcccc gccatcgaaa gtgcgcatgc tctcgccggc acgctgcacc tcggtcgtca
     2461 attagggccg gacgccgtgg tgctggtgag cctgtcaggt cggggtgaca aggacgtcga
     2521 cactgcggcg aagtggttcg acctagagtc ggcgtcgggt tcggcgtcag gttcggcgtc
     2581 aggttcggcc gcgccggtgg cggcgctgcg atgacggttc tgcccacgga gcaatccgag
     2641 gccgctgtga cttcggcggc acccgctgcc gtgccggtga ccgccgtccc gagccggctg
     2701 agtgcgacgt tggcccggtg caaggccgaa ggccgcgcgg cgttgatcgg ctacctgccc
     2761 gcgtgcttcc cgaaccccga gcgtggcatc gcagccctac tcgccctcgt cgaaggcggc
     2821 gtcgatgccg tcgagatcgg gcttccttac agcgacccgg tcctcgaggg cccggtcatc
     2881 gcgcgggcac atgacgtggc gctgcgagca ggttcgacga ccgatcgggt gttgcgcacg
     2941 gtcgaagctg tcgctgccac cggcgttgcc actgtcgtca tgacgtactg gaatccgatc
     3001 gagcagtacg gcgtcgaccg cttcgctgct gctctcgccg cggcgggtgc cagcggactc
     3061 gtcacacccg atctgctgcc cgacgaggcc gagccatgga tcgctgcgag cgatgcccac
     3121 ggtctcgacc gcatctttct cgtcgccccg agcagcaccg acagccgcat cgccctgact
     3181 gtgcgtcact gtcgcggttt catctacgca gcagctgtga tgggagtgac gggcacccgt
     3241 gcgcagacca gttcattggc gccgcccctc gtcgatcgca tccgggcgat cacgtcgctg
     3301 ccggtcggcg tcggcctcgg tgtcagcaac gccgatcagg ccgccgagat tgctggatac
     3361 gccgacgccg ttattgtcgg ctcggcgttt gtgcggcggg tcctcgaggc gcgcggaccc
     3421 caggacgaag ccgtcgccgt gcgctcgctc gccactgaac tggcagccgg tgttcggcgc
     3481 ggatagcgtc gacactgtca tgaagccaca tagccggacg tcgcgatgat ccagctcgct
     3541 gttttgccgg cttacatccc gagtccgagc gatggcgtct ggcatctcgg tccaatcccg
     3601 atccgcggct acgccctgtg catcatcgtc gggatcttcg cggcggccgt gctcgccgac
     3661 cgccgctacc gcgaccgtgg cggagcgatc ggcgtcatca acgaggtgtc gacgtgggcg
     3721 gtgccgttcg gacttgtggg tggccgcatc taccacgtgc tgaccgactg gtccgactac
     3781 ttcggcaaag gcggcagccc gatccgctcg ctcgaggtgt ggaagggagg cctcggcatt
     3841 cctggcgcgg tcgcgctggg cggccttggc gttttggtcg cctgccgcag gcgtggcgtc
     3901 ctgatgccgc cgatcgccga cgccatcgca cccgggctgg tgctcgcgca ggggatcggc
     3961 cgctggggca actggttcaa ccaggagttg tacggccggc cgagcgggct gccgtgggcg
     4021 gttcggatcg acacgaacca ccaagtaggc gtgattctga ccaagtacca ggagagcccg
     4081 tacggcttct accagccgac gttcctctac gagtcgatct gggatatctc gacagcggtg
     4141 atcctgatcc tgctcgaccg ccgctacaag ctcggccacg gccgggtctt cgcgctgtac
     4201 ctagtcatct acggcctcgg ccgcggctgg atcgaggcgc tgcggatcga tccggcgcac
     4261 cacttcttcg gcctgcgact caacgactgg accagcatca tcatcgtcgt cgctggtgtg
     4321 gtcggctaca tcgtctcggc ccgtcgccac ccagggcgtg aagtcgacct cgttcgggca
     4381 cccgtcgctg ccctgccaac cgagcccgcg gcagccgagg ccgacgccgc agccgcagcc
     4441 gacgacgctg ccgccgatga cgccgccgcc gaggtgacgg tcggatccga cgccgaccac
     4501 gacgcaccac cgggtgccag tagtggtggc agccatgaag gagcgggcac atgacgacgg
     4561 cagacgagac cgaggccgcc cagccggtca agccgaccgc tgagatccac cacgaccacc
     4621 gcgacgtcac gggcgggtgg ttgcgccccg cagtgttcgg ggcgatggac ggcctgatct
     4681 cgaatttctc cttgatctca ggcttttccg gggccaacgc ctcacgacac ctgctgatcc
     4741 tgagcggtct cggcggtcta gtcgccggcg cgttctcgat ggcggtcggc gagtacgtgt
     4801 cggtcgcctc tcagggagac ctcgcgcgcg ccgagatcga ggtcgaacgc cacgagcttc
     4861 ggcacaacgc ggcagccgaa caggccgagc ttgccgacct ctacgtgtcg cgcggcgtcg
     4921 accgcgcgct cgcgctcgaa gtttccaagc agctgtcacg cgacccagac caggcattgg
     4981 cgatccacgc tcgcgaagaa ctcggcatct ccgcggacgc gctgccctcg ccctacctc
//
LOCUS       DASWVF010000797         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_302707, whole genome shotgun sequence.
ACCESSION   DASWVF010000797 DASWVF010000000
VERSION     DASWVF010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302707"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..840
                     /locus_tag="VGD55_14115"
     CDS             142..840
                     /locus_tag="VGD55_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0871529.1"
                     /translation="MQAFVSLWSADLLALRDAVDQVDDVVDGFHIDVFDGHNADHLLF
                     GPDVVAALRQTTTKTIDVHLNVDDPGRWIDRFAEAGADTITVQSAPCPDVGEVLSQIR
                     ERGCAPGLGVEVHEAIDVAVPFLDVLSRVLLLGTPIGVKGRDLDPATADRIAALIAAR
                     ELAGVRPSIIVDGGIRKHTAPMLAAAGADGVIPGALVYGDRDPRRAASEIAKLEVGTA
                     HPVLSAFWSEEVSS"
     gene            857..>1609
                     /locus_tag="VGD55_14120"
     CDS             857..>1609
                     /locus_tag="VGD55_14120"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0871530.1"
                     /translation="MKAAVVTEPGVLVITDVPAPVPRDHEVIVQVDTCGICGTDVHVL
                     DGDYGVVRYPVIPGHEFAGTVTEVGAAVRGLEVGALVAVDPMDYCDACEMCRSGWTNM
                     CTNGGGLGTTAPGALAEYVAVNGARCEPLPPGLSLPLASLVEPLSCVLHALDRLGPVL
                     GADTLVLGSGPIGLMLTSLLSLAGATVDVVDRNPARLAFAPKFGATRIGSAAAELAAP
                     NWSVIVDATGNPLAVQDAFAFARRTARIALLGV"
BASE COUNT          232 a          535 c          542 g          300 t
ORIGIN      
        1 agcttggaac cgccgatgtc aacccctacc gtcgccacgg cggacaggct actttcgttt
       61 cggctcgttt gtatacctag tcgagccaac tcgaatcaaa tcgctcggct tcgagcggct
      121 tcgggcagga gggtggccgg cgtgcaggcg ttcgtgtcgc tgtggtccgc ggacctgctc
      181 gcgctgcgcg acgccgtaga ccaggtcgac gacgtcgtcg acgggttcca catcgacgtc
      241 ttcgacgggc acaacgctga ccacctgctg ttcggccccg atgtcgtggc cgctctgcgg
      301 cagaccacga cgaagacgat cgacgttcac ctcaacgtcg acgaccccgg ccgctggatc
      361 gaccgattcg cggaagccgg cgccgacacc atcaccgttc agtccgcgcc gtgtcctgac
      421 gtcggcgaag tgctctcgca aatccgcgaa cgcggctgcg cccctgggct cggagtggag
      481 gtgcacgaag cgatcgacgt cgccgtgccc ttcctcgacg tcctgtctcg tgtcctgctg
      541 cttggcacgc cgatcggtgt gaagggtcgc gacctggatc ccgcgaccgc cgatcgcatt
      601 gctgcgctga tcgccgcgcg tgagctagcc ggcgtccggc cgtcaatcat tgtcgacggt
      661 gggatccgca aacacaccgc gccgatgctc gccgctgcgg gtgccgacgg agtgatcccc
      721 ggcgccctcg tctacggtga ccgcgatccc cgacgggccg cgtccgagat cgccaagctc
      781 gaggttggca cggcacatcc ggtgctatcc gcattttgga gtgaggaagt gtcatcgtga
      841 gtgaggaggt gtcgccgtga aggccgcggt cgtcactgaa cccggcgtgc tcgtcatcac
      901 cgacgtgccg gcgccggtgc cacgcgatca cgaggtgatc gtgcaggtcg atacgtgcgg
      961 catctgcggc accgatgttc atgtgctgga cggcgattat ggcgtcgtgc gctatccggt
     1021 gatccccggt cacgagttcg ccggcacggt gacggaagtc ggagcagcag ttcgcggact
     1081 cgaagtcggg gcgctggtcg cggtcgatcc gatggactac tgcgacgcgt gcgagatgtg
     1141 ccggtccgga tggaccaaca tgtgcacgaa cggcggcggt cttggcacca cggcgcccgg
     1201 tgcactggcc gagtatgtag cggtcaacgg cgcccgctgc gaaccgctgc cacctgggct
     1261 gagtcttccg ttggccagcc tcgtcgagcc gctgtcgtgc gtgctgcacg cgctcgaccg
     1321 gctaggcccg gtgcttggcg ccgacaccct cgtactcggg tcaggcccga tcggcttgat
     1381 gctcacgtcg ttgttgtcgc tggctggggc gacggttgac gttgtcgatc gcaaccctgc
     1441 ccggcttgcc ttcgcgccga agttcggcgc cactcgcatc ggttcggcag ctgctgaact
     1501 ggcggcgccg aactggtcgg tgatcgtcga tgcgaccggc aacccgctgg ctgtgcagga
     1561 cgcgttcgcg tttgcccgcc gcaccgctcg catagccctg ctgggcgtc
//
LOCUS       DASWVF010000798         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_456282, whole genome shotgun sequence.
ACCESSION   DASWVF010000798 DASWVF010000000
VERSION     DASWVF010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456282"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VGD55_14125"
     CDS             complement(<1..762)
                     /locus_tag="VGD55_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0871531.1"
                     /translation="MQFFGRTAVATLGLSLIAVTGCATTARKTATDLEGVPLHVVAAE
                     NFWGSIATQLGGARVDVQSIITNPDTDPHDYDPSAADARDIANANVVIENGIGYDPWV
                     GKLIAADRPGDFQLVNVGDVVKVADGGNPHRWYNPTDVHSVIAAITAAYDAASPADAK
                     YFAAQQKTLETTGLAQYTSLIDQIKTTYAGTPIGASESIFAMLAPALGLNLITPPTFL
                     RAISEGTDPTSADKQTIDNQIKTHAIKVYVYNAQNA"
     gene            886..1290
                     /locus_tag="VGD55_14130"
     CDS             886..1290
                     /locus_tag="VGD55_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0871532.1"
                     /translation="MTTTNSGRSTRQKRALAVVIDDAQEFRSAQELHEALRGRGEYVG
                     LTTVYNQLRALADAGLVDTMRSPEGEILYRRCGSAGHHHHLLCRVCGRTVEVAGTAVE
                     RWAEKVAAEAGFVDVSHTIEVVGTCSACALLV"
     gene            complement(1287..1712)
                     /locus_tag="VGD55_14135"
     CDS             complement(1287..1712)
                     /locus_tag="VGD55_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871533.1"
                     /translation="MIRRSTLTLVAGVTLVVTLATGCAGHRNSVTPAAQNTTPTTAPV
                     APVSSVAPASSAAPVIAGASSAVAVAPPAGTTTTPAAPAPTTAPSADQAPANQAAAAA
                     NASSLAAINKDLAGIDAGSSAADKDMSAGDSARAQNDNG"
     gene            complement(1709..>2195)
                     /locus_tag="VGD55_14140"
     CDS             complement(1709..>2195)
                     /locus_tag="VGD55_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871534.1"
                     /translation="KTTLLATLNGDVTGLTALGVTIAGDTTAKQAASDYATIFTSYRV
                     YALALPQVRFAAAADDMTVTVLPKLTDAQTKLAALLAGVDSGKNTPQVQAWMADLSTQ
                     ITAIGTETNGLSATVLGYTPAQYDANHALLAPARASLGISRNDIKTARADIANVVKAL
                     K"
BASE COUNT          320 a          677 c          766 g          432 t
ORIGIN      
        1 cgcgttctgc gcgttgtaga cgtacacctt gatcgcgtgc gtcttgatct gattgtcgat
       61 cgtctgcttg tcagccgacg tcgggtcagt gccttcgctg atcgcgcgta agaacgtggg
      121 cggcgtgatc aggttcaggc ccaatgccgg cgccagcatc gcgaagatgc tctcggacgc
      181 cccgatcggt gtgccggcgt aggtggtctt gatctgatcg atcaggctgg tgtactgcgc
      241 aagccccgtg gtctcgagcg tcttttgctg ggcagcgaaa tacttcgcgt cggcagggct
      301 ggcggcatcg tatgcggccg tgatcgcggc gatgacactg tgcacgtccg tcgggttgta
      361 ccaacggtgc ggattcccgc cgtccgcgac cttgacgaca tcgccgacgt tgaccaactg
      421 gaagtccccc ggacgatcgg ccgcgatcag cttgcccacc cacgggtcgt agccgatgcc
      481 attctcgatg accacgttgg cgttcgcgat gtcgcgcgcg tcagccgcgc tcgggtcgta
      541 gtcgtgtggg tcggtgtctg gattcgtgat gatgctctgt acatcaaccc tcgcaccgcc
      601 gagctgcgtc gcgatcgagc cccagaagtt ctccgctgcc acaacgtgta gcggcacacc
      661 ctcgagatcc gtggccgtct tgcgggctgt cgttgcgcag ccggtgacag cgatcagcga
      721 caggccgagc gtggctaccg ccgtccggcc gaagaactgc acttgtccac cccgtcacct
      781 caggttccgc ttatctggaa ccgttgcagg tacactatcg ggaatggtta ccgtttccaa
      841 gtgcagttcg tcgccgccgg gccgtcggct agatttaggg tcgctatgac gacgacgaac
      901 agcggccggt cgacccgcca gaagcgcgcg ctcgccgtgg ttattgacga tgcgcaagag
      961 tttcgcagtg cgcaggaatt gcacgaggcg ctgcgcgggc gcggcgagta cgtcggcctg
     1021 acgaccgttt acaaccaact gcgggcgctc gccgacgctg gtctcgtcga cacgatgcgg
     1081 tcgcccgaag gcgagatcct ctaccgccgg tgcggcagtg ccggccacca ccaccacttg
     1141 ctctgccggg tgtgcggccg gacggtcgag gtcgcgggta ctgcggtcga gcgctgggcc
     1201 gagaaggtcg ccgccgaagc cggattcgtc gacgtttccc acacgatcga ggtcgtgggc
     1261 acctgctcgg cctgcgctct cctcgtttaa ccgttgtcgt tctgagcacg cgccgagtcg
     1321 ccggcgctca tgtccttgtc ggcggccgat gatccggcgt cgatgcctgc gaggtccttg
     1381 ttgatcgcgg cgagactgct ggcgttagct gctgccgcgg cttgattggc cggtgcttga
     1441 tcggccgacg gcgcggtggt cggggctggt gccgcgggcg tcgtggtggt accggccggt
     1501 ggagcaacag ctactgcaga gctcgccccc gcgatcacgg gcgctgccga tgaagccgga
     1561 gctaccgacg aaaccggagc taccggagct gtcgtcggcg tggtgttctg cgctgcgggc
     1621 gtgacgctgt tgcggtgtcc cgcacacccg gtggccagcg tcacgaccag cgtgacgccg
     1681 gcaaccagcg tgagggtgga cctgcgaatc acttcagcgc cttgacgacg ttcgcgatgt
     1741 cagcgcgagc ggtcttgatg tcgttgcgtg agatgccgag cgacgcgcgg gcaggtgcga
     1801 gcagcgcgtg gttcgcgtcg tactgtgccg gcgtgtaacc gagtacggtc gccgaaaggc
     1861 cgttcgtctc ggtgccgata gcggtgatct gcgtggagag gtcggccatc cacgcctgca
     1921 cttgcggggt gttcttgccc gagtcgacgc cggccagcaa cgcggcgagc ttggtttgcg
     1981 cgtcagtgag ctttggcagg acggtgaccg tcatgtcgtc ggccgctgct gcgaaacgca
     2041 cctgcggcaa tgcgagggcg tacacgcggt agctggtgaa gatcgtcgcg tagtcggacg
     2101 ccgcttgctt ggccgtggtg tcgccggcga tggtcacacc aagcgcggtc agaccggtga
     2161 cgtcaccgtt gagggtggcg agcagcgtgg tcttg
//
LOCUS       DASWVF010000799         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_201193, whole genome shotgun sequence.
ACCESSION   DASWVF010000799 DASWVF010000000
VERSION     DASWVF010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201193"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VGD55_14145"
     CDS             <1..176
                     /locus_tag="VGD55_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871535.1"
                     /translation="QVFERFYQVGGDRRRFGGVGLGLYIVRRLLETQGGTVAALPRDG
                     GGTCFEVSLRVAD"
     gene            282..674
                     /locus_tag="VGD55_14150"
     CDS             282..674
                     /locus_tag="VGD55_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871536.1"
                     /translation="MSSLRVAATPASVGYLRAVLRDDLAVLPEPVREEVALVASELLG
                     NAVRHARALDDGQLTVAWGVGDYGVEIAVTDGGGPTIPVARDAGPTETRGRGLSIVAT
                     LAARWGVERHESSTTVWAVVPFTGTQSR"
     gene            912..1355
                     /locus_tag="VGD55_14155"
     CDS             912..1355
                     /locus_tag="VGD55_14155"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0871537.1"
                     /translation="MSYLLIPLIQRPSPELAPLRETLRAWVTVVTTSADPCLVLNRDG
                     RVAGLSASAAALVAESPIDVVGRRLVCDVFTLIDFSSAAEPIADESLVPPTQAIATGV
                     LSRGLVRLRRADGVTLTLDAIATPLHADDGSVIGSLTFLGQLAAH"
     gene            complement(1320..2459)
                     /locus_tag="VGD55_14160"
     CDS             complement(1320..2459)
                     /locus_tag="VGD55_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871538.1"
                     /translation="MTVVRALALAGGCVVLAGTASSVLRTLVVPRGVLSRFSEIVLDS
                     TVRLSRIVADRIDDFDRRDNILAWAAPLSLMALLLSWIITFWLGFSLLDFGFTHFTIA
                     NAMRQAGSSIATLGFAASPTPSLTILDILTGITGMVVVALQIAYLPTLYSAYNRRETL
                     ITVLSARAGEPAWGPEILARHAWLNLLPNMPELYSDWESWAATVSESHTSYPVLIHFR
                     SPQPLRSWLISLLAMMDAAALQTALAPATAPIEARLMLRMGYLALRDIAAIESVPTRT
                     DVSPDDPIALPFEEFERAVEMLQGIGMTIERTAAEAWPHFRGWRVNYEEGAYALAKHI
                     DAVPAPWSGPRRHAEKPIAPLRPENRKPTVPAAGLVSGELAEEGQ"
     gene            2537..>3103
                     /locus_tag="VGD55_14165"
     CDS             2537..>3103
                     /locus_tag="VGD55_14165"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY0871539.1"
                     /translation="MSLNSSTAALLAVIGTSAGGLGLLVGAFAHLRLRRLRRDLAVLQ
                     AGDDAESFLAAAARQVEHIARLRAELSSVEGRMHDLRVDVADALRHVAVVRYDAFRDM
                     GGRMSFSAALLDDAGDGLVISAIHGRAETRSYAKGVKGGTSDQQLSPEERQAIAFATK
                     SDAGHAGRQPTAVQPDSPAVQIKRVVADE"
BASE COUNT          483 a          989 c         1059 g          572 t
ORIGIN      
        1 cccaggtctt cgagcgcttc taccaggtcg gtggtgatcg ccggcgattc ggcggcgtcg
       61 ggctcggcct ctacatcgtg cggcgactgc tcgagacaca gggcggcacg gtcgcggccc
      121 tgccgcgcga tggcggcgga acttgcttcg aagtgtcgct gcgagtcgcc gactaggttt
      181 gacacgattt gaccagggaa cgtacgcggc gggtcgtcag caccagtatt tgcggccagc
      241 gccgtagttt gcgcacgcgc ttagcgcggg aaggtgattg ggtgagttct ctgcgtgtcg
      301 cggccacccc ggccagtgtc ggctaccttc gagcggttct tcgtgatgat cttgctgtgc
      361 tcccagagcc ggttcgcgaa gaggttgcac tggtcgccag tgaattactc ggcaacgcgg
      421 ttcggcacgc gcgcgcgctc gatgacggtc agttgacggt cgcttggggc gtcggcgact
      481 acggcgtcga gatcgcggtg accgacggcg gcggaccgac catcccggtc gctcgcgacg
      541 ccggcccgac agagacacgc ggtcgaggat tgtcgatcgt tgcgacgttg gcggcgcgct
      601 ggggtgtcga gcgccacgag tcctcgacga cggtgtgggc ggtcgtgccg tttaccggca
      661 cgcaatcccg ctgaagctgc ttccctgcgg gtcgggttct cgcgcgtttg cgcggccgct
      721 gtgcgccgaa tgggcgcgcc cgtgacggta cctatgcctg gtgtctatcg agcgtgctct
      781 gtgtcacgct aaggatcgga caccactgtg gggacaatgg cgttcgacga cagcatgggg
      841 atctcgaaac cgggggaatt taggtgaccg accttgacgt tgctgtcgtt cctgagccta
      901 gacacgatgg catgtcgtat ctgctgattc cgctcatcca gcggccgtcg ccagagctcg
      961 ctccgctgcg cgaaaccttg cgcgcctggg tgaccgtcgt gacgacatcg gcggatccct
     1021 gcctggtgct caatcgtgac ggccgtgtcg ccggcctgtc tgcttctgcg gccgccctgg
     1081 tcgctgaatc acccatcgac gtggtcggtc gccgtctcgt gtgcgacgtc ttcaccctga
     1141 tcgacttcag cagcgcggcc gagccgattg ccgacgagtc gctcgtgcca ccaacccaag
     1201 cgatcgcgac cggcgtgctc tcccgaggcc ttgtccggct tcgtcgggcc gacggcgtca
     1261 cgctgactct cgacgccatt gccacgccgc tgcacgctga cgacggctcg gttatcgggt
     1321 cactgacctt cctcggccag ctcgccgctc actagtccgg cagcgggaac ggtcggcttg
     1381 cggttctcgg gccgcaacgg agcaatcggc ttttccgcat ggcgccgcgg ccccgaccac
     1441 ggtgctggca ccgcgtcgat gtgcttcgcc agtgcgtacg ccccctcttc gtagttgacc
     1501 cgccagcccc gaaaatgcgg ccacgcctcc gctgcagtgc gctcgatcgt catgccgatt
     1561 ccttggagca tctcgaccgc ccgctcaaac tcttcgaacg gcagcgcgat cggatcatcg
     1621 ggtgagacat cggtgcgggt cggcaccgac tcgatcgccg cgatgtcacg caaggccaga
     1681 tatcccatgc gcagcatcag gcgggcttcg atcggtgcag tcgcgggcgc tagcgcggtc
     1741 tggagtgctg cggcgtccat catggcgagc agcgagatga gccacgatcg cagcggctgg
     1801 ggcgacctga aatgaatcag cacggggtag ctggtgtggc tctccgaaac tgtcgcggcc
     1861 cagctttccc agtcgctgta aagctcgggc atgttcggaa gcaggttcag ccatgcgtgc
     1921 cgggccagga tctccgggcc ccacgctggc tcacctgccc gtgccgagag aacagtgatc
     1981 agcgtttcgc gccggttgta tgccgagtac agcgttggca agtaggcgat ctgcaaagcg
     2041 acgacgacca tgccggtgat cccggtcaag atgtcgagga tcgtgagcga cggcgtcggt
     2101 gaggcagcga agccgagcgt ggcgatcgac gagccggcct ggcgcatggc gtttgcgatc
     2161 gtgaaatggg tgaagccgaa atcaagcagg ctgaagccga gccagaacgt gatgatccag
     2221 ctcaagagca gcgccatcag cgacagcggt gctgcccacg caaggatgtt gtcgcggcgg
     2281 tcgaagtcat cgatgcggtc agcgacgatc cgcgacagcc gcaccgtgga gtcgagcacg
     2341 atctcggaga accgcgagag cacaccgcgt gggaccacca gggtacggag cacgcttgag
     2401 gctgtcccgg cgagcaccac gcagccgcca gcgagcgcta gcgcgcgcac gaccgtcatg
     2461 tcccgggacc ttagtggcag gagcagcagc gcctgagtcg gagtccctcg ggctgggtgc
     2521 cgttaggctg gccgccgtgt cgttgaacag ttcgactgct gccctgcttg ccgtgatcgg
     2581 gacgtcggcc ggtgggctcg gtctgctcgt cggcgcattc gcccatctgc gattgcgcag
     2641 gttgcgtcgc gatcttgctg ttcttcaggc cggcgacgat gcagaatctt tcctcgccgc
     2701 ggcggcccgg caggtggagc atatcgcccg gctgcgcgcg gaactgtcgt cggtcgaggg
     2761 tcgcatgcac gatctgcggg tcgacgtcgc tgacgcgtta cgacacgtcg cggtcgtgcg
     2821 ctacgacgca tttcgcgata tgggtggccg gatgtcgttc tccgccgcgt tgctcgacga
     2881 cgccggtgac gggcttgtca tcagcgcgat ccacggtcgg gccgagacac ggtcgtatgc
     2941 caagggcgtc aagggcggta ccagcgacca gcagttgtcg cccgaagaac gccaggcgat
     3001 tgccttcgcc accaagtcgg acgccggtca tgccggacgc cagcccaccg ccgtccagcc
     3061 tgattctccg gccgtgcaga tcaagcgggt tgtggctgac gag
//
LOCUS       DASWVF010000800         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_328010, whole genome shotgun sequence.
ACCESSION   DASWVF010000800 DASWVF010000000
VERSION     DASWVF010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328010"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="treZ"
                     /locus_tag="VGD55_14170"
     CDS             complement(<1..1388)
                     /gene="treZ"
                     /locus_tag="VGD55_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY0871540.1"
                     /translation="MSDMRVWAPGASEVELHVDAERRSMTRGERGWWAVEAPPGSRYG
                     FSLDGGDVLPDPRSQWQPDGVRGLSALVDHDAFTWRTANWRGREVLGSVIYELHLGTY
                     TEGGTFDAAIPRLDHLVELGIEFVELLPVAAFPGRHGWGYDGVFPYAVHAPYGGPDGL
                     KRFVDACHERGLAVILDVVYNHLGPDGNVLPRFGPYFTDTYSTPWGSALNYSGPASDE
                     VRRYVIDNALMWLRDYRVDALRLDAVHMMLDTSATHILEELATEVSALAERVGRPLAL
                     VAESDLNDPRLIVARDAGGYGLDTQWSDDFHHALHSVVTGETSGYYEDFGSLRQLATA
                     LTRGYVYAGEYSVHRDRVHGRALPASVTGHRLVGFAQDHDQVGNRAAGERLSALVSPG
                     LVKVAAALVLTSPFTPMLFMGEEWAASTPWQYFTDHEDTELAEAVRTGRRGEFKAFGW
                     RPEDVPDPQHPTT"
BASE COUNT          273 a          520 c          525 g          257 t
ORIGIN      
        1 gtggtggggt gttgcgggtc aggtacgtcc tccggtctcc agccgaacgc tttgaactca
       61 cctcgccggc cggtgcgcac cgcttcagcc aactcggtgt cttcgtgatc ggtgaagtac
      121 tgccagggag tcgacgctgc ccactcctca cccatgaaca gcatcggggt gaacggcgag
      181 gtcaggacga gcgcggccgc gaccttcaca agtccgggcg acaccagtgc cgacaagcgc
      241 tcgccggccg cacggttgcc gacctggtca tggtcttgcg caaagcccac caaccggtgg
      301 cccgtgaccg acgcgggcaa cgcgcgaccg tgcactcgat cgcgatgcac cgagtactcg
      361 cctgcgtaaa cgtaccctcg ggtcagtgcg gtggctaatt gccgcaacga cccgaaatcc
      421 tcgtagtagc cgcttgtttc gcccgtcact acggagtgca gcgcgtggtg aaagtcgtca
      481 ctccattgcg tatcgaggcc atagccgccg gcgtcgcggg caacgatcag ccgcggatcg
      541 ttgaggtcgg attcagcgac cagtgcaagc ggtcggccga cccgctcggc aagcgcgctc
      601 acctcggtcg cgagttcttc gaggatgtgg gtcgccgacg tgtcgagcat catgtggacg
      661 gcgtcaaggc gcagggcatc gacgcggtag tcgcgcaacc acatcagcgc gttgtcgatg
      721 acgtaccggc gcacttcgtc cgacgctggc cccgagtagt tgagcgcgct gccccagggc
      781 gtcgagtagg tgtcggtgaa gtacgggccg aacctcggca gcacgtttcc gtcggggccg
      841 aggtggttgt agacgacatc gagaatgacg gcgaggccac gttcgtggca cgcgtcgacg
      901 aaacgcttga gcccgtcggg gccgccgtac ggcgcatgca ccgcgtacgg aaagacgccg
      961 tcataacccc acccgtggcg acccgggaat gccgccaccg gaaggagttc gacgaattcg
     1021 atgcccagct cgacgaggtg gtcgagccga gggatcgccg cgtcgaacgt gccgccttcg
     1081 gtataggtac cgaggtgcag ttcgtagatg acgctgccga gcacctcgcg acctcgccag
     1141 ttcgccgtgc gccaggtgaa ggcgtcgtga tcgacgaggg cagaaagtcc gcggacgccg
     1201 tcgggttgcc actgtgatct cgggtcgggc agcacgtcgc cgccgtcgag cgagaacccg
     1261 tacctcgaac cgggtggcgc ctcgaccgcc caccaaccac gttcgccgcg agtcatggat
     1321 cgccgctcgg cgtcgacatg cagctcgacc tcggaagcgc cgggcgccca cacgcgcatg
     1381 tcactcatga cgttgcaacg atcacgtgac gatgactcct ggaaccagca acgcgaccgg
     1441 gctgtgccta aggagcgcgc ctactggtgt ctcgccggct tcgtgttcga gtccgctgag
     1501 ggcgtcgacc caggtgtggc ctggcactgc acctaccggc agctcgagca ccgtgtcgcg
     1561 ccagccgccg ctgcg
//
LOCUS       DASWVF010000801         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_354188, whole genome shotgun sequence.
ACCESSION   DASWVF010000801 DASWVF010000000
VERSION     DASWVF010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354188"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VGD55_14175"
     CDS             <1..1075
                     /locus_tag="VGD55_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871541.1"
                     /translation="CEDQRGCLRVSEPEPPPWQPPQPPYQQYPPPGQPWPPPGQPYQQ
                     AGPPPPPWAAGYPVGPPPNPYAYAPASRYTYAPPSHRGRNVVIGSIVATLVIVVAVVE
                     ITASQSPQTQVSAGKAAAVAHSPTAPAASNPPNPAGGITASSPLDSPLGTSVIFSDSF
                     GDPNSGWDTGADSDSNYTYTPGGYSMTTNAPGVFFSYAPDDTPAQQVSLAVTAIESPG
                     ADPDAGYGASCRRGPDSASQLRYEFRVTGSGQWFIERNTGDLDAQTPTIVDQGSSQLL
                     AGTQGTLSAVCATLADGTTTRLALFLNGTKVADTVDVATADGAGWLGAILSTNPGTAP
                     ATTVVRYYTDSALGPLPIIAANT"
     gene            1122..>1746
                     /gene="ricT"
                     /locus_tag="VGD55_14180"
     CDS             1122..>1746
                     /gene="ricT"
                     /locus_tag="VGD55_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEY0871542.1"
                     /translation="MGMMMAVSFTRYGRLYYLDPGPHAPKVGDKVLFPTTAGDEVAEC
                     IWAPTYVSEEIGGLPMCAGYATSDHLARDERNRARKAEARVTTKRLVRQHELPMKVVG
                     VDYLDSDNSFVIYFSAPERVDFRALVRDLARTLNARIELRQLGARDEVRVQGGIGPCG
                     RDTCCSTFLKDFEPVSVRMAKDQDLPPNPLKISGACGRLMCCLKYEHP"
BASE COUNT          286 a          610 c          554 g          296 t
ORIGIN      
        1 atgtgaggat caacgtgggt gcctgcgcgt gagcgagccc gaaccgccgc cctggcagcc
       61 accgcagccg ccgtatcagc agtaccctcc gcccggtcag ccgtggcctc cacccggtca
      121 gccgtaccag caggccggtc cgccgcctcc gccgtgggcc gccggctacc cggtcggacc
      181 gccgccgaac ccctacgcgt acgccccggc gtcccgctac acctacgcgc caccgtcgca
      241 ccgcgggcgc aacgtggtca tcggctctat cgtcgcgacg ctcgtcatcg tcgtcgcggt
      301 agtggagatc accgcctcgc agtcgccgca gactcaggtt tcggcgggca aagctgcggc
      361 ggtcgcgcat tcgccgacgg cacctgccgc ttcgaacccg ccgaaccccg cgggcggcat
      421 cacggcgtcg tcgccattgg actcaccgct tggcacgtcg gtgatcttct cggattcttt
      481 cggcgacccg aactcgggct gggacaccgg tgccgatagc gattcgaact acacctacac
      541 acccggcggc tactcgatga cgaccaatgc gcccggcgtc tttttctcgt acgcacccga
      601 cgacaccccc gctcagcaag tgagcctggc ggtcaccgcc atcgagtctc cgggggcgga
      661 tcctgatgct ggctacggcg cttcgtgccg tcgcgggccc gactcggcat cgcagcttcg
      721 ctacgaattc cgcgtcaccg gctcgggaca gtggttcatc gagcgcaaca caggcgatct
      781 cgatgcccag acgccgacga tcgttgatca agggtcgagc caactactcg ctgggacgca
      841 agggaccctg tccgcagtct gcgcgacgct tgccgacggc accacgacac gactcgcgtt
      901 gttcctcaac gggacgaagg tggccgacac agtcgatgtg gcgaccgccg atggcgcggg
      961 gtggctcggt gccatcctga gcacgaatcc gggtaccgcg cccgcgacga cggtcgtgcg
     1021 ctactacacc gactccgccc tcgggccgct tcctatcatc gcggcgaaca cctaggtagg
     1081 cttgcgcgct gcggtctctc ggcgcggtct agagtcaccc catggggatg atgatggccg
     1141 tcagctttac ccgctacggc cggttgtact acctcgaccc cggtccgcat gcgccgaaag
     1201 tcggcgacaa ggtgctcttc ccgacgaccg ccggcgatga ggtcgcggag tgcatctggg
     1261 cgccgaccta cgtgtcggag gagatcggcg gcttgccgat gtgcgccggc tacgcgacgt
     1321 ccgaccatct tgcacgcgac gagcgcaatc gtgcgcgcaa agccgaagcg cgggtgacca
     1381 cgaagcgcct cgtacgtcag cacgaactgc cgatgaaggt ggtaggagtc gactatctcg
     1441 actccgacaa cagtttcgtc atttacttca gcgctcccga gcgcgtcgat ttccgagcgc
     1501 tggttcgtga cctcgcacgc acgctgaacg cgcgcatcga gctgcgtcag ttgggcgcgc
     1561 gcgacgaagt gcgggtgcaa ggcggcatcg ggccgtgtgg tcgcgacacg tgctgctcga
     1621 ccttcttgaa agacttcgag ccggtcagcg ttcggatggc gaaagaccag gatctgccgc
     1681 cgaatccgct gaagatttcc ggtgcgtgcg ggcggttgat gtgctgcttg aaatacgagc
     1741 acccgc
//
LOCUS       DASWVF010000802         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_48185, whole genome shotgun sequence.
ACCESSION   DASWVF010000802 DASWVF010000000
VERSION     DASWVF010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48185"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGD55_14185"
     CDS             complement(<1..463)
                     /locus_tag="VGD55_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HEY0871543.1"
                     /translation="MSLHSVVVASAITVGDHPTFTLFGLTFNRDTLESTGVAGAIVLI
                     LGLMVARGANSRNPTKIQLVFEMLVDWVNKQVEESMGIAVAPFVVPFSVALFFFILVS
                     NWLEVIPSGHNPEYLPAPTADVNLTYALALLVIIPVHILSIRRRGIRGYIAH"
     gene            complement(460..858)
                     /locus_tag="VGD55_14190"
     CDS             complement(460..858)
                     /locus_tag="VGD55_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871544.1"
                     /translation="MDDGRTVRYVVGAPRRVFVVAMVLGGVGLAAASIGGHWLVGLLF
                     VVGLLLGAVHNTTTATAIRKATNAGGEPDKRAFAFSSLFRLLYITVFAALFLVVFHRA
                     GLAVFVGLLVFHFLALVGTGVPLIKELKKG"
     gene            complement(862..1092)
                     /locus_tag="VGD55_14195"
     CDS             complement(862..1092)
                     /locus_tag="VGD55_14195"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0871545.1"
                     /translation="MTTDRQRSNLGGFELLGIGIFNALCLAAGLGLGWFADREIGSSP
                     VCTVVGLFVGLGAGAVGTWMQLKRYIDVDRSK"
     gene            complement(1127..2236)
                     /locus_tag="VGD55_14200"
     CDS             complement(1127..2236)
                     /locus_tag="VGD55_14200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY0871546.1"
                     /translation="MREYLITFLVAAAVTYFSTPIVRRFAVRIGAMTSVRDRDVHSIP
                     TPRLGGAAMFAGMAAALLVASRMPTLQVVNEKFSEPRALLLGGGVVFVIGALDDRFNL
                     DAVTKLAGQILAASVIVLQGVQLLWLPIPGSGTLILGQDWSVILTVTFIVIVINAVNF
                     IDGLDGLAAGVVGIAALAFFALSYNLARGAHLGALPPVLIAIIVAGICLGFLPHNFHP
                     ARVFMGDSGAMLIGLLLAASTVSFTGQVNVVTQYQQYGAFLPLLLPFAILLVPFADLV
                     LAVARRTKAGRAPWAPDKQHLHHRLLELGHSQRRAVLIMYFWSALFGAGVVALSVTKG
                     PLVVVSLGAALAAVALLLVNMPRLRAARRARHGAA"
     gene            complement(2288..2938)
                     /locus_tag="VGD55_14205"
     CDS             complement(2288..2938)
                     /locus_tag="VGD55_14205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0871547.1"
                     /translation="MTVYDCDDPAQRAMGIREAAYAVRRGDLVVLPTDTVYGIGADAF
                     TPAAVNALLAAKGRGRDMPVPVLIGSRTVLDALVGEISEPARHLVEVLWPGALTLVFQ
                     HTPALAWDLGDSRGTVALRMPADLLCLELLKETGPMAVSSANRTGLPPATTCANAQAQ
                     LGEAVAVYLDTGPTTVSVPSSIVDVTGPVPVLVRPGWISIERLREIVPDLQEARAV"
     gene            complement(2935..>3532)
                     /locus_tag="VGD55_14210"
     CDS             complement(2935..>3532)
                     /locus_tag="VGD55_14210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY0871548.1"
                     /translation="PRPETEVVAGWCVDNLREPARSHSPIVVDLCTGSGAIALSIAQE
                     VRGATVHAVERESAAHEWAQRNATGTRVLVHHADASEALAELEGTVDLVVANPPYLPE
                     TDRDQVDPEVRDHDPDAALWGGPDGLDGPRMIEAAARRLLRDGGLVAVEHADHHGSSV
                     ADMFRASGGWAHIVVRQDLAGRDRFVTARRDVSQEGSA"
BASE COUNT          685 a         1187 c         1158 g          502 t
ORIGIN      
        1 agtgcgcgat gtaaccgcgg attccgcgtc ggcgaatgct caggatgtgt accgggatga
       61 tgaccagcag cgccagcgca taggtcaggt tgacgtcggc cgtgggtgcc ggcaggtatt
      121 cggggttgtg accgctcggg atcacctcga gccagttcga caccaggatg aagaagaaga
      181 gggcgactga gaacggcacg acgaacggcg cgaccgcgat gcccatgctt tcttcgacct
      241 gcttgttgac ccagtcgacg agcatctcga acacgagctg aatcttcgtc ggattacgac
      301 tgttcgcacc gcgggcgacc atcaacccga ggattaagac gatcgcgccg gccaccccgg
      361 ttgactccaa ggtgtcgcgg ttgaacgtca acccgaaaag tgtgaacgtc ggatggtcgc
      421 cgacggtgat ggccgacgcc accacgacgc tgtgcaggct catcccttct taagctcctt
      481 gatcagcggc acgccagtgc cgaccaacgc gaggaagtga aagaccagca gcccgacgaa
      541 gacggcaagc ccggcgcgat ggaagaccac caggaagagt gccgcgaaga cggtgatgta
      601 gaggagtcga aaaagcgagg agaaggcgaa ggctcgctta tcgggctctc ctccggcatt
      661 cgtggccttg cggatggccg tcgcggtcgt ggtgttgtgc accgcgccga ggagcagccc
      721 gacgacgaac aacaagccca caagccaatg cccgccgatg gacgccgcgg ccaggccaac
      781 tccacccagc accatcgcta cgacgaaaac acgcctgggc gcgccgacga cgtatcgaac
      841 ggtccgcccg tcgtccactt cttacttgct cctgtcaacg tcgatgtacc gcttcagctg
      901 catccacgtg cccaccgccc ctgcgccaag gccgacgaag agcccgacga cggtgcacac
      961 cgggctgctg ccgatctcac gatcggcgaa ccacccgagt ccgagcccgg cagccagaca
     1021 caaagcattg aaaattccaa tgccgagcaa ctcgaatccg ccgaggttag acctttgccg
     1081 atccgtggtc atcttagggg cttcatgcgg tgagagcgtg cccgggtcat gccgccccgt
     1141 gccgggcacg acgcgccgcc cgcaggcgcg gcatgttcac caacagcagc gcgacggcgg
     1201 ccaacgccgc gcccagtgag accacgacga gcggcccttt cgtcaccgag agcgccacga
     1261 cgccagcccc gaaaagggcc gaccagaagt acatgatcaa caccgcgcgg cgctgggagt
     1321 gtccgagttc gagcaaccgg tggtgcaggt gttgcttgtc gggcgcccat ggtgcccgcc
     1381 cggccttggt ccgccgggcg accgcgagca ccaggtcggc gaagggcacc agcaagatcg
     1441 cgaatggcag cagcaacggc agaaacgcgc cgtactgctg gtactgcgtg acgacattga
     1501 cctgcccggt gaacgacacg gtcgacgccg ccagcagcag gccgatcagc atggcgccgg
     1561 agtcgcccat gaacacccgc gcggggtgga agttgtgcgg caggaaccca aggcaaatgc
     1621 cggcgacaat gatcgcgatc agcaccggcg gcaaggcgcc gagatgggct ccccgcgcaa
     1681 ggttgtacga gagcgcgaag aacgctagcg cggcgatgcc gacgaccccc gccgccaggc
     1741 cgtcgagccc gtcgatgaag ttgacggcgt tgatcacgat gacaatgaac gtgacggtca
     1801 agatgaccga ccagtcctgc ccaaggatca gcgtgcccga gcccggaatt ggcagccaca
     1861 gcagttgtac gccctgcaac acgatgaccg acgccgcaag gatctgaccc gccagcttgg
     1921 tcacggcgtc gaggttgaag cggtcgtcaa gggccccgat gacgaacacc acgccgccgc
     1981 cgagcaagag cgcccgcggt tcggagaact tctcgttgac gacctgcagc gtcggcattc
     2041 gcgaggcgac gagcaaagcc gcggccattc cggcgaacat ggctgctcca ccgaggcggg
     2101 gcgtcgggat cgagtgcacg tctctgtcgc ggacgctcgt catcgcaccg atccgtaccg
     2161 cgaaccggcg aacgatcggc gtggagaagt aggtgaccgc cgcggcgacc aggaacgtga
     2221 tcagatactc gcgcacggaa ctcctcgtgg gcggcggtgt cgatcatcga cggcgctcac
     2281 tctacggcta gaccgcgcgt gcttcctgca ggtcaggcac gatctcgcgc aagcgctcga
     2341 tggagatcca gccgggccgc accagtacgg gcaccgggcc ggtgacgtcg acgatcgatg
     2401 acggcaccga gaccgtggtc gggcccgtgt cgaggtaaac cgcgaccgct tcacccagtt
     2461 gtgcttgcgc attcgcgcat gttgtcgctg gtggcagccc ggtgcggttg gcgctgctga
     2521 ccgccatcgg acccgtctcc ttcagcaact caagacacag caggtcggcc ggcatccgca
     2581 gcgcgacggt gccgcgagag tcgccgagat cccacgccag cgccggcgtg tgctggaaaa
     2641 cgagggtcag cgcgcccggc cacaacacct caacgagatg ccgcgcgggt tctgagatct
     2701 cacccacgag cgcgtcgagc accgttcgcg agccgatcag cactggcacg ggcatgtcgc
     2761 gcccccggcc cttcgctgcc aagagcgcgt tgacggccgc cggcgtgaac gcgtcggccc
     2821 cgatgccgta gaccgtgtcg gtcggcagca ccacaaggtc gccgcggcga accgcgtacg
     2881 cagcctcgcg gattcccatc gcgcgctggg cggggtcgtc gcagtcgtaa accgtcatgc
     2941 gctgccctcc tgcgacacgt ctcgacgcgc ggtcacgaac cggtcgcgac cagcaagatc
     3001 ttgccggacg acgatgtgcg cccaccctcc ggatgcgcgg aacatatcgg ctaccgaaga
     3061 cccatggtgg tcggcgtgct caaccgcgac gaggccgcca tcgcgcagca agcgccgggc
     3121 ggccgcctcg atcatccgcg gcccatcaag accgtctggg ccaccccaca atgcggcgtc
     3181 cggatcgtga tctcgcactt ccggatcgac ctgatcgcga tcggtctcag gcaggtaggg
     3241 cgggttcgcg acgacgagat cgacagttcc ctcgagctcg gcgagcgcct cggaggcgtc
     3301 ggcgtggtgg acgaggacgc gggtaccggt ggcgttgcgc tgcgcccact cgtgcgcggc
     3361 cgactcacgt tcgaccgcgt gcacggtcgc gcccctgacc tcctgggcga tcgacagcgc
     3421 gatcgcgccc gaaccggtgc acaagtcgac gacgatcggt gagtgcgaac gggccggctc
     3481 gcgaagattg tcgacgcacc aacccgcgac gacttcggtc tcgggccggg gc
//
LOCUS       DASWVF010000803         6230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_302891, whole genome shotgun sequence.
ACCESSION   DASWVF010000803 DASWVF010000000
VERSION     DASWVF010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302891"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGD55_14215"
     CDS             <1..556
                     /locus_tag="VGD55_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0871549.1"
                     /translation="AYLLRCTATGDTMLIDAAAEPDRLLTERGDGLLVSVVTTHQHPD
                     HWQGLEAVVGVTGPAVIAHRLDAVGLPIPMTETVADGDVLGVGNVQVKVIHLKGHTPG
                     SIAILYDADPAAPHLFTGDSLFPGGPGNTRKNVADFTSLMNDLEAKVFGVLPDATWVY
                     PGHGADTTLGAERPHLGEWRARGW"
     gene            complement(642..866)
                     /locus_tag="VGD55_14220"
     CDS             complement(642..866)
                     /locus_tag="VGD55_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871550.1"
                     /translation="MPKRRTPQWYRLGGLLWAIVAALYWIAEIVQQKPLHLALGILFT
                     CVAVTYFVGAKELARRSAARLASRWQLPDK"
     gene            complement(901..1176)
                     /locus_tag="VGD55_14225"
     CDS             complement(901..1176)
                     /locus_tag="VGD55_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0871551.1"
                     /translation="MKYVLIYESRADLDLGQLRAVFPEHRARWAVFQEQGTLLLIGPY
                     ADRAGALAVFTTQEAAEEFAATVPFVTNGLVAKWEIKPWAEALLEPL"
     gene            complement(1186..1419)
                     /locus_tag="VGD55_14230"
     CDS             complement(1186..1419)
                     /locus_tag="VGD55_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871552.1"
                     /translation="MSAARGSAGKGRAGKFFVVTLLVAAAAGASSHPAPRRLARTAID
                     YGRAWSKRQLDRSLQAPVIKSQGERWRAMHPDA"
     gene            complement(1539..3647)
                     /gene="uvrB"
                     /locus_tag="VGD55_14235"
     CDS             complement(1539..3647)
                     /gene="uvrB"
                     /locus_tag="VGD55_14235"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0871553.1"
                     /translation="MRPVTDLVRSVAPLQVVSDFTPSGDQPTAIDDLERRIKRGDRDA
                     VLLGATGTGKSATTAWLVERLQRPTLVMAPNKTLAAQLANELREMLPNNAVEYFVSYY
                     DYYQPEAYIPQTDTYIEKDSSINDEVERLRHSATNSLLTRRDVVVVASVSCIYGLGTP
                     QEYVDRMVRLKVGQEIERDKLLRKLVEIQYTRNDVAFTRGTFRVRGDTIEIIPQYEEL
                     AVRIEMFGDTIERLSTLHPLTGELVTEDQELYVFPASHYVAGPERMERAIAGIEAELE
                     QRLAELEKQGKLLESQRLRMRTTYDLEMMRQVGSCSGIENYSRHIDGREAGTAPNTLL
                     DYFPEDFLLVIDESHVTVPQIGGMYEGDMSRKRNLVDHGFRLPSAMDNRPLRWEEFVD
                     RIGQTVYLSATPGPYELGRVQGDVVEQVIRPTGLVDPEIVVKPSKGQIDDLVHEIRLR
                     ADRNERVLVTTLTKKMSEDLTDYLLELGIRVRYLHSEVDTLRRVELLRELRLGEFDVL
                     VGINLLREGLDLPEVSLVSILDADKEGFLRSGTSLIQTIGRAARNVSGQVHMYADIVT
                     ASMAHAIDETNRRRAKQVAYNVERGIDPQPLRKKIADILDSIVREDVDTQALIGGGGR
                     QMSRGKAPVPGLSSRTAGIHARELAGMPREELGELIAQLTEQMHTAATELHFELAARL
                     RDEIHELKKELRGMEAAGVR"
     gene            complement(3746..4147)
                     /locus_tag="VGD55_14240"
     CDS             complement(3746..4147)
                     /locus_tag="VGD55_14240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0871554.1"
                     /translation="MSTIVFALAGWMLAACASSGHASGSASDLSGVQSSPIASSSASA
                     QGTVTGVVRSYGGPLMPDGKMADDGNPTSGVTVTATQDGKTVASTVTGTDGSYRFTLP
                     SGSYVLTGCSNATVVASAGLLVHQDLRCDVP"
     gene            complement(4592..5215)
                     /gene="coaE"
                     /locus_tag="VGD55_14245"
     CDS             complement(4592..5215)
                     /gene="coaE"
                     /locus_tag="VGD55_14245"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0871555.1"
                     /translation="MPALTVGLTGGLGAGKSVVLAEFVELGALGIDADDVAREVVLPG
                     TIGFDQVVATFGSEVVGSDGWLDRGRLATVVFADAEERARLEAIVHPLVRAETRRRIA
                     AASPDAIVVYAAPLLVELDLAGEYDAVVVVQAPVSLRLQRVFDARAMSASDALARIGV
                     QATDEERAAVAWRLIVNRGTLDELRDEVDRVWGDLQLLRKWRTELPG"
     gene            complement(5223..5951)
                     /locus_tag="VGD55_14250"
     CDS             complement(5223..5951)
                     /locus_tag="VGD55_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871556.1"
                     /translation="MSVVAGRAFAVALVAVCVTGCTTTKSGTAPTPVVSHSIAPTSPP
                     PPVASETASVGSSASSSPSAPVLPSGCSQLLPLGTLEKILGFGILGQVNYLKAAPVPQ
                     SGRTGRVTCSYGNSIGQIPASPAASPSKASASPAPPPLLQISYITYVDAKTAASRVQA
                     TVEADGASAVVTDVTVNGKPANVLIGSTSSELVMSDGARTIVVVALATVLTPDKAADG
                     LESIAEAMLRFGQPPASPSPASTS"
     gene            6155..6230
                     /locus_tag="VGD55_14255"
                     /pseudo
     CDS             6155..6230
                     /locus_tag="VGD55_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
BASE COUNT         1064 a         2136 c         1974 g         1056 t
ORIGIN      
        1 cgcgtacctg ctgcgctgca cggcgaccgg cgacacgatg ctcatcgacg cggccgccga
       61 acccgaccgg ctgctgaccg aacgcggcga cgggctgctc gtcagcgtcg tcacgaccca
      121 tcagcatccc gaccactggc aaggtctcga agcagtcgtc ggagtcaccg gtccggcggt
      181 catcgcacat cggctcgacg ccgttggact accgattcca atgacagaaa cagtggcgga
      241 cggcgatgtg ctcggcgtcg ggaacgttca ggtgaaggtc atccatctga agggccacac
      301 cccgggcagc atcgcgatcc tctacgacgc cgacccggct gcgcctcact tgttcaccgg
      361 cgactcgttg tttccgggcg gcccaggcaa tacgcgcaag aacgtcgctg acttcacgtc
      421 cttaatgaac gacctcgagg ccaaggtgtt cggcgtcctg cctgatgcaa cgtgggtcta
      481 cccaggccac ggcgccgaca caacgttagg tgccgagcgc ccgcacttag gtgagtggcg
      541 cgcccgcggc tggtgagccg ccaacgccgc cgacttggaa gtcctaggac gcccaagatc
      601 gcgtctacag gcttcctact tcgcgtcgcc gccgggtgcg tctatttgtc gggtagttgc
      661 cagcggctcg ctagccgggc ggcagaacga cgagctagct ccttggcgcc cacgaagtag
      721 gtgacggcga cacaggtgaa caggatcccg agggcaaggt gcaacggctt ctgctgaacg
      781 atctcggcaa tccagtacaa cgccgcaacg atcgcccaga gcagaccacc aaggcgatac
      841 cactgtgggg tgcgtcgttt cggcacagcc acacgctaca gcgcgcccgc gcgcccgcga
      901 ctagagcggc tcgaggagcg cctcggccca cggcttgatc tcccatttgg cgacgaggcc
      961 gttggttacg aaggggacgg tcgccgcgaa ctcctcggca gcttcctggg tggtgaacac
     1021 cgcgagcgcg ccggcgcggt cggcgtaggg gccgatcagc aacagggtgc cctgctcctg
     1081 gaacacggcc catcgcgcgc ggtgctcagg aaagactgca cgcagctgtc ccaggtctag
     1141 gtcagcacgc gactcataga tcagcacgta cttcacgaac cggccctacg cgtcggggtg
     1201 catggcgcgc caccgctcgc cctgcgactt gatcaccggc gcttgcaggg agcgatccag
     1261 ctggcgcttc gaccaggccc ggccgtagtc gatggcagtg cgtgcaaggc gacgtggcgc
     1321 cggatggctg ctcgccccag cagctgcagc gaccaggagc gtcaccacga agaacttccc
     1381 ggcgcgcccc ttgcccgcgc taccacgagc ggcgctcatg cgacacccca aacgaagacc
     1441 ggtgagctag ctgtgggacc tagcgagccg acgataccga tggacggccg atttcaacat
     1501 gatttgttgc ggcagccact agtcggtgaa tccggcgatc agcggacgcc ggcggcttcc
     1561 atcccgcgaa gctctttctt gagctcgtgg atctcgtcac gcaaccgggc cgccagctcg
     1621 aagtgcagct cggtcgcggc cgtgtgcatc tgctcggtca gctgggcgat cagctcgcca
     1681 agttcctcgc gcggcatgcc agcgagctcc cgcgcgtgga tgccggccgt ccgcgatgac
     1741 agcccaggca ccggcgcctt cccgcggctc atctgccgac cgccaccgcc gatcagggct
     1801 tgagtgtcga cgtcctcgcg aacgatcgag tccaggatgt cggcaatctt cttccgcagc
     1861 ggctgcggat cgatgccgcg ctcgacgttg taggcgacct gcttcgcccg ccggcggttg
     1921 gtctcgtcga tcgcatgtgc catcgaggcg gtgacgatgt cggcgtacat gtggacctgc
     1981 cccgacacgt tgcgcgcggc gcggccgatc gtctggatca gcgaggtgcc cgaccgcagg
     2041 aacccttcct tgtcggcatc gaggatcgac accaacgaca cctcaggcag gtcgaggcct
     2101 tctcgaagca ggttgatgcc gaccagaacg tcgaactcgc caaggcgcaa ctcgcggagc
     2161 agctcgaccc ggcgcaacgt gtcgacctca ctgtgcaggt agcgcacccg gataccgagc
     2221 tcgagcaggt agtcggtcag gtcttcggac atcttcttcg tcaacgtcgt gacgagcacg
     2281 cgttcgttgc gatcggcccg caaccggatc tcgtgcacga ggtcgtcgat ctgccccttg
     2341 ctcggcttca ccacgatctc ggggtcgacc agcccagtcg gccggatgac ctgctccacg
     2401 acatcgcctt gcacccggcc caactcgtac ggcccaggag ttgccgacaa gtagacggtc
     2461 tggccgatgc ggtcgacgaa ctcttcccag cgcagcgggc ggttgtccat cgcgctcggc
     2521 aaccggaagc cgtggtcgac gaggttgcgc tttcgggaca tgtccccttc gtacatgccg
     2581 ccgatctgcg gaaccgtcac gtgcgactcg tcgatgacca gcaggaagtc ttcagggaag
     2641 tagtcgagca aggtgttcgg cgcagtgccg gcttcgcgac cgtcgatgtg ccggctgtag
     2701 ttctcgatgc ccgagcaact gccgacttgc cgcatcatct cgaggtcgta ggtcgtgcgc
     2761 atgcgcaggc gttgtgactc gagcaacttg ccttgcttct cgagctctgc gagccgttgc
     2821 tcgagttcgg cctcgatgcc ggcgatcgcc cgctccatgc gctcaggccc ggcgacatag
     2881 tgcgaagccg ggaacacgta caactcttga tcttcggtaa ccagctcgcc cgtcaacgga
     2941 tgcagggtcg acagccgctc gatggtgtca ccgaacatct cgatgcgcac ggccagttct
     3001 tcgtactgcg ggatgatctc gatcgtgtcg ccgcgcaccc gaaacgtgcc acgggtgaac
     3061 gcgacgtcgt tgcgggtgta ctggatctcg accagcttgc gaagaagctt gtcgcgctcg
     3121 atctcttggc ccactttgag cctgaccatg cggtcgacgt actcctgcgg agtgccaagc
     3181 ccataaatgc acgacacgct cgccaccacg acgacgtcgc gccgcgtcag cagactgttc
     3241 gtggccgagt gccgtaaccg ctcgacctcg tcgttgatcg acgagtcctt ctcgatatag
     3301 gtatcggtct gcgggatgta ggcctcgggc tggtagtagt cgtagtagct cacgaagtac
     3361 tcgaccgcgt tgttcggcag catctcgcgc agctcgttgg ccagctgggc cgccagcgtc
     3421 ttgttcggcg ccatgaccag ggtcggccgc tgaagccgct cgaccagcca cgctgtcgtc
     3481 gccgacttgc ccgtgccggt cgcgccgagc aggacggcgt cacggtctcc gcgcttgata
     3541 cggcgctcca aatcgtcgat cgcggtcggc tgatcgcctg acggcgtgaa gtcgctgaca
     3601 acctgcagcg gcgccacgct gcgcactaga tcggtgactg gtctcacgga gaacaccgta
     3661 cgtggtcgtc ccgacagttt cctccgcccg cagaatcggt cagcgttttc ctccgcccgc
     3721 agaatcggtc agcgccgtca ggacttcacg gaacgtcgca acgcaggtcc tggtgcacaa
     3781 gcaggcctgc cgacgccacg actgtcgcgt tggagcaccc ggtcaggacg tagctcccgg
     3841 acggcaaggt gaaccggtag ctgccatcgg tgccggtgac cgtcgaggcg accgtcttgc
     3901 cgtcctgggt agcggtgact gtgacgcccg aggtcgggtt tccgtcgtcc gccatcttgc
     3961 cgtcgggcat gagcggacca ccgtacgacc gcaccacgcc agtgacagtt ccttgcgcgg
     4021 acgccgacga gctggcgatc ggtgaacttt gcacaccact caaatcggac gccgaaccgc
     4081 tggcgtgacc cgaactcgcg cacgccgcaa gcatccaacc cgccagcgcg aagacgattg
     4141 tggacactgg tccgctcgga ttcatggcct ttcgacgtcg atgcacgcat cacggttgca
     4201 cgatcgccgg caacttcaca cccgctctgg ttgctgaaac cgcccaaagc acctcggcca
     4261 acagccacac gaggtgtgaa gttggccgga ccgtgcgacg gccggggcgg gtgtgactct
     4321 cagcgttcgt tggtgccaca gttgccaaag attctcgcct aattcgcttc cctgtaagga
     4381 attcctcccc taacgtttgg tctcgcaaga agtgatccgc tgcttctccg ggcgaccggg
     4441 gaagacccgc tgacggcctc ggccgagggc ggggcgcggg gagccacttg caatgtgacc
     4501 ccccgggcct cggcccgggg ggtcgcgtaa ttcacgggtg ccgcgtagtt cggccggcgt
     4561 aactccgcgc acgtaagttc gccgcggcag ttcagcctgg gagttcggtt cgccacttcc
     4621 gcagcaactg caagtcgccc cacacccggt cgacctcgtc gcgcagctcg tcgagcgtgc
     4681 cgcggttcac gatcagcctc cacgccacgg ctgcgcgttc ttcgtcggtg gcctgcacac
     4741 cgatccgcgc gagagcgtcc gacgccgaca tcgcccgggc gtcgaagacc cgctggagcc
     4801 gcagcgacac cggtgcctgc acgaccacca cagcgtcgta ctcccccgcc agatccagct
     4861 ccaccagcaa cggcgccgcg tacaccacga tcgcgtcagg gctggcggcc gcgatccgcc
     4921 gcctcgtttc cgcccgcacc aggggatgca cgattgcctc gagacgcgcc cgctcctcgg
     4981 cgtccgcgaa aaccaccgtc gcgagacgcc cgcggtcgag ccagccgtcg ctgccgacga
     5041 cctccgaccc gaacgtcgcc acgacctggt cgaacccgat cgtcccgggc aggacgacct
     5101 cgcgagcgac atcgtcagcg tcgatgccga gggcacccag ctcgacgaac tcggccagca
     5161 cgacgctctt gcccgcgccg aggccgcccg tgagcccaac ggtcaaagct ggcatggtcg
     5221 cctcacgacg tgctcgcggg cgagggtgat gcgggtggtt ggccgaacct caacatggcc
     5281 tcggcgatgg attcgaggcc gtccgccgcc ttatcgggcg tgagcaccgt cgcgagcgcg
     5341 acgacaacga tcgtgcgtgc cccatcactc atgaccagct cactcgacgt cgacccgatc
     5401 agcacgttcg ccggcttgcc gttgaccgtc acgtcggtca ccaccgcgga ggccccgtcg
     5461 gcctcgactg tggcctgaac ccgagacgcg gccgttttcg cgtctacgta ggtgatgtag
     5521 gagatctgca acagcggcgg gggcgctgga gaagcacttg ccttggacgg cgaggcagct
     5581 ggcgacgctg gaatctgacc gatcgaattg ccgtaactac aggtcacccg gccggtgcgt
     5641 cccgattgcg gcaccggcgc cgccttcaag tagttcacct gcccgagtat cccgaagccg
     5701 agaatcttct ccaacgtccc caacgggagc agttgcgagc accccgacgg cagcaccggg
     5761 gcggacggcg acgatgacgc actcgaaccg accgacgcgg tctcgctcgc gaccggtggc
     5821 ggcggcgacg ttggggctat cgagtgactc acgacaggtg ttggcgcggt ccccgatttc
     5881 gtggtggtgc agccagtcac gcacacggcg acgagcgcta ccgcgaacgc acgccctgcg
     5941 acgactgaca tggccgcagg gtaatgcttg cagggcacca aaaacggcgg gcctcgccga
     6001 agcgagcccc gccggattcg ttcgagaaga cggtcagctc tcgccgctcg tcagcttccg
     6061 tcgcagctct tctaattggg cgtcggaagc caggctgccc tcggccgcct cgggttcggc
     6121 gtcggttgcg gtcgtcgcag cgacctcggg aaccggcgtc gacggtctcg tccacgtctc
     6181 cgagctgtcg tggaagcaca tcgaccaccc gagtgaagtg gtcgaggttg
//
LOCUS       DASWVF010000804         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_456478, whole genome shotgun sequence.
ACCESSION   DASWVF010000804 DASWVF010000000
VERSION     DASWVF010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456478"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGD55_14260"
     CDS             complement(<1..544)
                     /locus_tag="VGD55_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0871557.1"
                     /translation="MLDTRDAAAGRSTLLADYTTIRLGGHATTLVWADDEPALIEQVT
                     RAEADGTPVLVLGGGSNLIVADAGFDGVVVRVASHGTRVDRDGEQLVLHVAAGEIWDD
                     VVSGSLSQGLAGLECLSGIPGLAGATPIQNVGAYGCEISSLISAVRVLDRQQQRTVVL
                     TGADCRFGYRTSALRGGDRYV"
     gene            complement(575..1996)
                     /locus_tag="VGD55_14265"
     CDS             complement(575..1996)
                     /locus_tag="VGD55_14265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0871558.1"
                     /translation="MTGRGRPLATFIVTSVALFMSSLDNLVVTMALPAIKSHLHASLS
                     SLEWTVNAYTLTFAVLLLPGATLGDRFGRKKMFMIGLSIFTMASALAALSSTANELVA
                     ARALQGVGGAILTPLTLTLLSVAVPPSRRGLALGAWGAIAGLAIAVGPVVGGAIVEGA
                     SWSWIFWLNVPIGIVLLPVAWRLLKESHGPAAKLDVVGTGLATAGLFSVVLGLVKAND
                     WGWGSSSIIGLFVVGVVFLAAFVRWETRVAEPVLPLRLFRNRGFTASNVASLFMFFGM
                     FGSIFLLAQFLQVAHHYSPLSAGVRTLPWTAMPIFVAPVAGLLSDRIGGRRIVATGLF
                     LQAIGLFWLASVASPTVPYSHLVPGLAISGFGMALFFAPVANLVLSLVRHDEQGIASG
                     AHNAIRELGGVFGVAVLASVFARSGGYQSAQAFADGVVPAVKLGALIVALGGVAALLI
                     PRQPVLSPEPEAIEVSDLEPSAA"
     gene            complement(2100..>2433)
                     /locus_tag="VGD55_14270"
     CDS             complement(2100..>2433)
                     /locus_tag="VGD55_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0871559.1"
                     /translation="FTEAADGLAGEAALEAMGAAYVGLIADRENLMGQLQMYAACHDA
                     GVREFARDGFRKLYQLVEGVSGASAEELATFFAMGMLCNLSAALSLAEVDEPWAKALA
                     GHEKPAPL"
BASE COUNT          495 a          836 c          777 g          325 t
ORIGIN      
        1 cgacataacg atcgccgccg cgaagcgcac tcgtgcggta gccgaaccga cagtccgcgc
       61 cggtcaacac gacggtgcgt tgctgctggc gatcgagcac gcgaaccgcg ctgatcagcg
      121 acgagatctc acagccgtac gccccaacat tttggatcgg tgtcgctcca gcgagaccgg
      181 gaattcccga caggcactcg agtcctgcca gtccctgcga cagcgatccg ctcaccacgt
      241 cgtcccagat ctcacccgcc gcgacgtgca gcaccagctg ctcgccgtca cgatcgacgc
      301 gcgtcccgtg acttgcgacg cgcacaacga ccccgtcgaa accggcgtcg gcgacgatga
      361 gattgctgcc gccgccgagc acgagtactg gcgtgccgtc ggcctcggcg cgagtcacct
      421 gctcgatgag cgccggttca tcgtcggccc acaccagcgt ggtcgcatga ccgcccagcc
      481 ggatcgtcgt gtagtcggcc agcaacgtcg aacggcctgc cgcagcatcg cgagtgtcga
      541 gcacccacga accctacggc cggccacccg acattcaggc tgctgacggc tccaagtcgc
      601 tgacctcgat cgcctcaggc tccggagata agacgggctg gcgcggaatg aggagcgcgg
      661 ccacgccgcc gagcgcgacg atcagagcgc ccaacttgac ggccggcacc acgccgtccg
      721 cgaaagcttg cgcactttga taaccaccgg agcgcgcgaa caccgacgcg agcacggcaa
      781 cgccgaacac gcctccgagc tcgcgaatcg cattgtgcgc gcccgacgcg atgccctgct
      841 cgtcatggcg caccaacgac agcaccaggt tggcgaccgg cgcgaagaac agcgccatgc
      901 cgaacccgct gatcgcaagg ccgggcacca ggtgtgagta cggcacggtt ggagacgcga
      961 cgctcgccag ccagaacagt ccgatggcct ggaggaacag acctgtcgcc acgatgcgac
     1021 gcccgccgat gcggtcggac aacaaaccgg ccactggcgc gacgaagatc ggcatcgcgg
     1081 tccagggcag cgtgcggacg cccgccgaga gcggcgagta gtgatgcgcg acctggagga
     1141 actgcgcgag caagaagatc gagccgaaca tcccgaagaa catgaacagc gacgcgacgt
     1201 tgctagcggt gaatccgcga ttgcggaaca gccgcagggg caacaccggc tcggcgaccc
     1261 tcgtctccca gcggacgaac gcagcaagga acacgacacc cacgacgaac aagccgatga
     1321 tcgacgagct gccccaaccc cagtcgttcg ccttcaccag accgagcacg accgagaaca
     1381 gacctgctgt cgcaaggccg gtgcccacga cgtcgagctt cgccgccggg ccgtgacttt
     1441 ccttcagcaa ccgccaggcg accgggagca acacgatgcc gatcggcacg ttgagccaga
     1501 agatccacga ccacgacgca ccctcgacga tcgcgccacc gacgaccggc ccgacggcga
     1561 tcgcaagacc ggcgatcgca ccccaggcac cgagggcaag cccgcgccgc gacggcggca
     1621 ccgccacgct caacagcgtc agggtcaacg gagtgaggat cgcgccgccc acgccctgaa
     1681 gggcgcgtgc cgcgaccaac tcgttggccg ttgacgacaa cgcggcgagc gccgacgcca
     1741 tggtgaagat cgagagcccg atcatgaaca tcttcttgcg accgaagcgg tcgccaagcg
     1801 tcgcacccgg cagcagcagg acggcgaagg tgagggtgta ggcgttcacg gtccactcga
     1861 ggctcgagag cgaagcgtgc aggtgcgact tgatcgccgg cagcgccatc gtgacgacga
     1921 ggttgtcgag tgagctcatg aacaaggcaa ccgaggtgac gatgaatgtc gccagcggcc
     1981 ttccgcgtcc agtcatgtca tgtcctccgg ttcgtgtctt gcgctggtgt cagtttgggt
     2041 taagtgagta gccactaact tgcgtgggca aataaaaagc gttcggagca gcgggccatt
     2101 cagagtggcg cgggtttctc gtggccggcg agcgccttcg cccacggctc atcgacctcg
     2161 gccagcgaga gagcagccga gaggttgcag agcatgccca tcgcgaagaa tgtcgcgagt
     2221 tcttcggcag aggctccgga gactccctcg acgagctggt aaagcttgcg gaacccgtcg
     2281 cgcgcgaact cgcgcacgcc tgcgtcgtgg cacgcggcgt acatctggag ctggcccatc
     2341 aggttttcgc ggtcggcgat caggccgaca taagcagccc ccatcgcttc gagcgcagct
     2401 tcgccggcga gcccgtcggc ggcctcggtg aag
//
LOCUS       DASWVF010000805         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_73191, whole genome shotgun sequence.
ACCESSION   DASWVF010000805 DASWVF010000000
VERSION     DASWVF010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73191"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGD55_14275"
     CDS             <1..314
                     /locus_tag="VGD55_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871560.1"
                     /translation="AMAVSVSKSVFTLNGTGILMDACASADAPCLPIVDQFTSNRFGQ
                     NHGNGVTWGYGTVTMASNQFQDNSGWGFWAGLGTTVVDGGGNNAQGDNAGDCNGGLVC
                     S"
     gene            484..855
                     /locus_tag="VGD55_14280"
     CDS             484..855
                     /locus_tag="VGD55_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871561.1"
                     /translation="MKLVDASSAAAAAVAAAVVAPSSVDGAPGVLSVDDVGARIVDLS
                     GRLSATTCAWLLLIADFDTRQGYLSFGLASTAQWLSHACGIAHRTAVEHVRVAGSLAL
                     FPPTRGGDGRGPVVVFAGPRD"
     gene            887..>1707
                     /locus_tag="VGD55_14285"
     CDS             887..>1707
                     /locus_tag="VGD55_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY0871562.1"
                     /translation="MEDLIQVARHGTVAQLETVVRGLRTVDHNEPAADPGEYVKEAWT
                     SDSRCQLSARLDPERGAVVAAAIDAIVQRDGCTPAEALERVAQICLASLADAGNPPRD
                     LRGHERAAVVIHVDAARVRPRSAEPDPGDDPDDAPGDRVEVPLPRSAEPDPADAPSVA
                     RPYARVAGGPGLPDEVVRRLLCDGRIRTVIHDGPNVLDVGRSHRLVTDRQYRALLLRH
                     HNHCAHPGCPNAKNLHAHHRIHWIHGGRTDLDNLLLLCERHHLAHHCGEFEVFTLG"
BASE COUNT          257 a          561 c          599 g          290 t
ORIGIN      
        1 aagcgatggc agtgtcggtg agcaagtccg tcttcacgct gaatggaacc ggcatcttga
       61 tggacgcctg cgcgtcggca gacgcgccgt gcctgccgat cgtcgatcaa ttcacgtcta
      121 accggttcgg gcaaaaccac gggaacggcg tcacctgggg ctacggcact gtgacgatgg
      181 cgagcaacca gtttcaggac aacagcggct ggggcttctg ggccggcttg ggaaccaccg
      241 ttgttgatgg gggcgggaac aacgcccaag gcgacaacgc cggcgattgc aacggcggtc
      301 tcgtctgttc gtgagcgcgc aattcgctgc gctgacttgc ccggaagagg cggtcgccga
      361 ccagcgtcgt tcggccgcgt tcagctgaac gcagcgcggg gtgtcgcgtt cgcgttcatg
      421 tgaacgcggc actgcggaat gggtccgccc ggaagtgtcg gtgggtgtgg gtagcgtgcg
      481 ggcatgaaac tcgtcgatgc gtcttctgct gctgctgccg ctgttgctgc cgctgttgtt
      541 gccccttctt cggtcgatgg tgcgccaggt gtcttgtcgg tcgatgatgt gggtgcgcgc
      601 atcgtggatc tgtcggggcg gctgtcggcg accacatgcg catggttgct gctgatcgcc
      661 gacttcgaca cacgccaggg ttatctgtcg ttcgggttag catcgaccgc gcagtggttg
      721 agccacgcgt gtgggatcgc gcaccgcacc gcggttgagc atgtgcgcgt cgccggatcg
      781 cttgcgctgt ttcccccaac tcgcggcgga gatgggcgcg ggccggttgt cgttttcgca
      841 ggtccgcgcg attagccgcc tcgccgcacc gggtgagcac acgctggtgg aggatctgat
      901 ccaggtcgcc cggcacggca cggtcgccca gttggagacg gtggtgcgtg ggttgcgcac
      961 cgtggaccac aacgagcccg ccgccgaccc cggggaatac gtgaaggagg cgtggacctc
     1021 cgacagccgg tgccagctct cagcccgcct tgacccggaa cgcggcgcgg tggtggcggc
     1081 ggcgatcgac gcgatcgtgc aacgcgacgg ctgcaccccg gcggaagcac tggagcgcgt
     1141 cgcgcagatc tgcttggcga gcctggccga tgcgggcaac ccgccgcggg acctgcgcgg
     1201 gcacgaacgc gccgctgtgg tcattcatgt ggacgccgcg cgggtgcggc cgcgttcagc
     1261 tgaacccgac ccgggcgacg acccagacga cgcgccgggc gaccgggtcg aggtaccgct
     1321 cccgcgttca gctgaacccg acccggccga tgcgccgagc gtcgcgcggc cttacgcgcg
     1381 ggtcgcgggt gggccggggc ttcccgacga ggtggtgcga cggctgctct gcgacggccg
     1441 gatccgcacc gtgatccacg acgggcccaa cgtgctcgac gtcggccgat cccatcgcct
     1501 ggtcaccgac cggcaatacc gcgccctgct gctgcgccac cacaaccatt gcgcccaccc
     1561 gggctgcccg aatgcgaaga atctgcacgc gcatcaccgg atccactgga tccacggcgg
     1621 ccgcaccgac ctggacaacc tgctactcct atgcgaacgc caccacctag cccaccactg
     1681 cggtgagttc gaagtgttca ccctcgg
//
LOCUS       DASWVF010000806         6462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_227015, whole genome shotgun sequence.
ACCESSION   DASWVF010000806 DASWVF010000000
VERSION     DASWVF010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227015"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VGD55_14290"
     CDS             complement(<1..860)
                     /locus_tag="VGD55_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0871563.1"
                     /translation="MSAPAAGASDGWALEQLTAYLFGLADAGDPAAVLLEAVDEAAEA
                     MEAEVAVLIVDGSVVAAIGFANGAVPDDELLALAKDRPSVVELEGVGRCHVSVAQFDV
                     ERDCALKIGHSSALIVARTGEAAFSIAERNLLRGMSRALALNLQAARVLSAMNERQSL
                     MERLTRIQRSINSRAPLPEVLQAIVDGAAELIGDTVAGLRHVDPNDPDYAILSCAVNL
                     EPESPLGDRIRVGEGAGGRAISENQLVVMHDYAGRDDALPNWADLKLAAAMAAPVHEN
                     GVVVGSLVVG"
     gene            complement(857..1996)
                     /locus_tag="VGD55_14295"
     CDS             complement(857..1996)
                     /locus_tag="VGD55_14295"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0871564.1"
                     /translation="MGRASIAEPSMGSHALHLALDGRTPALVMAFASSELDLPLLVDD
                     LRRSVDADIPVVGCTTAGEISSDGPSDGGVVVVAFGGVDFSIATTVAENVSAGLRDAG
                     ALVASAVSSLDERQYRILLLLTDGLAGDQQEIVRGAYSVVGAQVPLVGGCAGDDQHMK
                     TTSVFYDGRVLTNAIVGIAIGSTAPFGIGVRHGWRRVGEPILVTSSIGTRVLTLDDEP
                     ALDVYLRRLDAPPEATTDPAAFARFAMTHPLGLSRRSGEEVRFIAGADFTDRSLHCIA
                     QVPQGGLAWIMDGEDTSVLAATDGACADALAQLGGEDAKGVLAFDCIARRGVLGDEGI
                     LREVEHITAGVRGAPVAGFYSYGEIARTKGTGGFHNQTLVVLAVS"
     gene            2197..3669
                     /locus_tag="VGD55_14300"
     CDS             2197..3669
                     /locus_tag="VGD55_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY0871565.1"
                     /translation="MTTLGRTEVEIEIVAEVREGEYGGKLAAIEPGGAEFIPLEERHG
                     NPLQLLWTWTSPNLEFATVFVGVLGVAAFGLSFWQATVALILGTLLGSITHAVLSAQG
                     PQFGVPQMILSRIPFGYRGNWLPAGLNSVTAGIGWFAVNSVSGAFALNTLTHMPKWLC
                     LVIIVAAQLTIAFFGHNLAHVVEKWALPVLGLAFLLAAISIFSKANAGAPHGPSASYG
                     LGGTGGFLLTLGGAFGYGVGWNPFASDYTRYMKPTSSKRAVGLFAGLGVFVSCVVLEM
                     IGAASATIGKSTDALNNPTSAFTGHMSTALADFVLVAIAIGAVCANVLNIYSGAMSFL
                     TLGFRLPLALRRAIVAAVAGVIGFIVALTGLHDAGEKYTNFLLVVAYWIAPWLGVMFS
                     DMLLRRRARVDGFLFDTKHNPWAGWVSMGIAMVVSIWLFSDQTKYLAPIPKAHPGLGD
                     ITFEVGFVLAAVLYAVFYKLQGGRKSETLVIPEYGAGARK"
     gene            complement(3911..5173)
                     /gene="codA"
                     /locus_tag="VGD55_14305"
     CDS             complement(3911..5173)
                     /gene="codA"
                     /locus_tag="VGD55_14305"
                     /inference="COORDINATES: protein motif:HMM:NF005748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HEY0871566.1"
                     /translation="MLDLLLRNARVRGSDGLTDLGVRDGIFVAPEPEARESIDVAGAL
                     VTPPLVEPHIHLDAVLTVGQPRPNKSGSLFEGIAVWSERVKDLTVDDVKSRVREVLRW
                     QLANGVQHVRSHVDVCDSNLTALRALVEIREEARGQVDLQLVAFPQLGIMSFDGGREL
                     MRKAVELGADVVGGIPHYELTREDGVESVKFTFDLAEEFGLRVDIHCDETDDDHSRFV
                     EVMAAETIRRGMSGRVTASHTTAMHSYNAAYAYRLVNNIKRAGLHMVTNPLDNAVLQG
                     RFDTGPIRRGHTRVKQLQEAGVNVCIGHDSVMDPWYPLGYGDPLQAAFVFVHLGHYSG
                     DDELHRLLDMVTVNPAAALGLEGYGIVEGTPADFVVFDAPTDVDAIRLGVRRLLVVRA
                     GRVVARTAPAQTTVTWAGVEQPVTFLRP"
     gene            complement(5186..>6462)
                     /locus_tag="VGD55_14310"
     CDS             complement(5186..>6462)
                     /locus_tag="VGD55_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0871567.1"
                     /translation="MRGRRSAGAVTAVLVTLGMLAGCSTKSNRSQDSRPPQGFALAPS
                     TSLSSAQVGAPSGAYSAPASSLMAAQLETPGGKPSGASSPYALAPQGGKTHRPTTTAA
                     GPIHPGSTQSVHATQQPANPALPALPAQAVVDVSVATLWHSPSSPRSVDAAELRQPAG
                     VQAWLSSMSTAQRLDLNGRVDSQLLLGEQVVVDQRQGTWAHVVVADQPTPLDASGYPG
                     WIHLAQLTFRTPTATSQELVVTSPSAWIYANDDVTQVVDVSMATRLPVLSDNTRWVQS
                     QLPDGRAVSLRAADVALVSPGAGPLAPTADDIVRTAKLFLGLPYLWGGTSGFGFDCSG
                     LTHLVFAMRGLIIPRDADAQARVGEAVDRADLRPGDLVFFASNGVVHHVGIYVGNDTM
                     LSSLQTGSPVEYSSLSAQPFASEYSGARRFVG"
BASE COUNT         1097 a         2155 c         2105 g         1105 t
ORIGIN      
        1 ctgcccacga cgagactgcc cacgaccaca ccgttctcgt gcaccggcgc ggccatggcg
       61 gcagcgagtt tcaggtcggc ccagttcggc agcgcatcgt ctcgacctgc atagtcgtgc
      121 atcaccacga gctggttctc cgagatcgcg cgaccgccgg caccttcgcc gacccttatc
      181 cgatcaccga gaggtgactc aggctcgagg ttgaccgcac acgagaggat cgcgtagtcg
      241 ggatcgttcg ggtcgacatg ccgcaaaccc gccaccgtgt caccgatcag ttcggcggcg
      301 ccgtcgacga tcgcctgcaa cacctcgggc aacggtgcac ggctgttgat cgaccgctgg
      361 atcctggtca gcctttccat caggctctgc cgttcattca tcgccgagag cacccgcgcg
      421 gcctgcaggt tgagggcaag cgcgcgactc atgccgcgca ggagattccg ctctgcgatc
      481 gagaaagcgg cttcaccggt gcgcgccaca atcaaggcgc tgctgtggcc gatcttcaac
      541 gcgcagtcgc gttcgacgtc gaactgggcc accgagacat gacaccggcc cacgccctct
      601 agctcgacga cactcgggcg gtctttcgcg agcgccagca actcgtcgtc cggcactgcg
      661 ccgttcgcaa agccgatcgc cgcgaccacc gacccgtcga cgatgagaac ggcgacctcg
      721 gcttccattg cctcggccgc ctcgtcgact gcttcgagca gcacggcggc ggggtcaccg
      781 gcgtcggcga ggccgaatag gtaggcggtg agttgctcga gcgcccaacc gtcggacgcg
      841 cctgcggccg gcgcgctcac gacaccgcga gaacgacgag cgtctggttg tggaacccac
      901 cggtgccctt cgtgcgggcg atctcgccgt agctgtagaa gccggcgacg ggcgcgcccc
      961 gaacgcctgc agtgatgtgt tcgacttcgc gcaggatccc ctcgtcgccg agaacgcctc
     1021 gacgtgcgat gcagtcgaag gcgagcaccc ccttcgcgtc ttcgccgcca agttgggcga
     1081 gcgcatcggc acacgcgccg tcggttgccg cgagtactga ggtgtcctcg ccatccatga
     1141 tccaagcgag accaccctgc gggacttgag cgatgcagtg caacgagcgg tcggtgaagt
     1201 cggcgcctgc gatgaagcgg acctcttcgc cgctgcgcct gctcaacccg agcgggtggg
     1261 tcatcgcgaa gcgggcgaac gccgctgggt cggtcgtcgc ctcgggcggg gcgtcaaggc
     1321 ggcgcaagta gacgtcgagc gcgggctcgt cgtcgagggt gagcacccgc gttccgatgc
     1381 tgctggtcac caggatcggc tcgccgacgc ggcgccaacc gtggcgcacg ccgatgccga
     1441 acggtgctgt cgacccgatc gcgatgccga cgatcgcgtt cgtgagaacc cggccgtcgt
     1501 agaacacgct ggtggtcttc atgtgctggt cgtcgcccgc gcatccgccg acgagcggca
     1561 cctgggcgcc gactacgctg tacgcgccgc gcacgatttc ctgctgatcg ccggcgagac
     1621 cgtcggtcag gagcaggagg atccggtatt gccgctcgtc gaggctgctg acggcacttg
     1681 ccaccagtgc gccggcgtca cgcaatccgg cgctgacgtt ctcggcgacc gtggtggcga
     1741 tcgaaaagtc gacgcctccg aacgcgacga ccaccacccc gccgtccgac gggccatcgg
     1801 aactaatctc gccggcggtg gtgcagccga cgacgggtat gtcggcgtcg acggatcgac
     1861 gcaggtcatc gacaagcaac gggaggtcga gctcggacga cgcgaacgcc atcacgagcg
     1921 ccggggtgcg gccgtcgagc gcgagatgaa gggcgtggct ccccatcgag ggctcggcga
     1981 tcgaggctcg gcccacgccg atccaacgac tggcggaggc ttgactcgac acggagtcgg
     2041 cctcggatct caacgtcgac gcgaccatgc acaccctgtc ggcaagggag ggcggattct
     2101 tactggtctt gcgcgagtga tccgaggtct tgggacttct ctccgtaaca gtttcaggct
     2161 actctccgcg ccaccagtcc agaaaggggc gctcgcgtga cgacattggg tcgcaccgaa
     2221 gttgaaatcg agatcgtcgc cgaggtccgc gagggtgaat acggcggcaa gctggccgcg
     2281 atcgaacccg gtggggccga gttcatcccg cttgaggagc ggcacgggaa tcctctccag
     2341 ctgttgtgga cctggacatc gccgaacctc gagttcgcca ccgtgttcgt cggagtgttg
     2401 ggcgtcgccg cgttcggcct ctcgttctgg caggcgacag tggcgctgat cctcgggacg
     2461 ctgctggggt caatcacgca cgctgtgctc tcggcacagg ggccacagtt cggcgtcccg
     2521 caaatgatct tgtcgcgcat tccgttcggc taccgcggca actggctgcc ggccggcttg
     2581 aactccgtca ccgcaggcat cggctggttc gcggtcaata gtgtaagtgg cgcattcgcg
     2641 ctgaacacgt tgacgcatat gccgaagtgg ctgtgcttgg tgatcatcgt ggccgcgcag
     2701 ctgacgatcg cgttcttcgg tcacaacctc gcacacgtcg tcgagaaatg ggcgctgccg
     2761 gttctcggtc tcgcgttcct gctcgcggcc atctccatct tcagcaaggc caacgcgggc
     2821 gcaccgcacg gcccgagtgc gtcgtacggg cttggcggaa cgggaggctt cctgctcacg
     2881 ctcggaggcg cgttcggata cggcgtcggc tggaatccct tcgcgtccga ctacacgcgc
     2941 tacatgaagc cgacgtcgtc caagcgtgcg gtcggactct tcgccggtct cggtgttttc
     3001 gtgtcctgcg tcgtgctcga gatgatcggc gcggcctcag ccacgatcgg caagtcgacc
     3061 gatgcgctga acaacccgac cagcgcgttc accgggcaca tgagcaccgc gctcgccgac
     3121 ttcgtgctgg tcgcgatcgc gattggcgct gtctgcgcga acgtgctgaa catctactcc
     3181 ggtgcgatgt cgttcctgac cttgggcttc cggcttccgc tcgcgttgcg gcgggcgatc
     3241 gtggctgcgg tagccggcgt catcggcttc attgtcgcgc tgactggttt gcacgacgcg
     3301 ggcgagaagt acaccaactt cttgcttgtg gtcgcgtact ggattgcccc gtggcttggt
     3361 gtcatgttct ccgacatgtt gttgcgccgc cgggcccgtg ttgacggatt cctcttcgac
     3421 accaagcaca atccatgggc cggctgggtg tcgatgggta tcgcgatggt cgtcagcatc
     3481 tggctgttct ccgaccagac caagtacctc gccccgatcc cgaaggcaca ccccggactc
     3541 ggcgacatca ctttcgaggt cggcttcgtg ctcgctgccg tgctgtacgc ggtcttctac
     3601 aagctccagg gcggccgcaa atccgagacg ttggtgatcc ccgagtacgg cgccggcgcc
     3661 aggaagtagg ccgcggccgg gacggcggcc accactcgcc gtcccgccgt cgagccctta
     3721 ctgcgccact cgccgccagc cctaccgcac cactcgccgc cagccctacc gcaccactcg
     3781 ccgtcgagcc ctaccgcacc actcgccgtc gagcccttac gaggcttaga cccccgagga
     3841 catgcgcaca tgcctgtcct cgggggccgc aaccccctga acgtgatcaa ctaccggccg
     3901 gggcgcgggg ttacgggcgg aggaaggtca cgggttgctc gacgccggcc caggtcaccg
     3961 ttgtctgggc cggcgcggta cgagcaacaa cacgaccagc gcggacgacg aggagccggc
     4021 gaactccgag cctgatcgca tcgacatcgg tcggagcatc gaagaccacg aagtcggcgg
     4081 gagttccttc gacgatgccg tacccctcga gacccaatgc ggccgcgggg ttcacggtca
     4141 ccatgtcgag taaccggtgc aactcgtcgt ccccgctgta gtggccgaga tgcacgaaaa
     4201 cgaacgccgc ctggagcgga tcgccgtaac caagcggata ccacgggtcc atcaccgagt
     4261 cgtgcccgat acacacgttg acgccggcct cttgcagctg cttgacacga gtgtggccgc
     4321 ggcggatcgg gccggtgtcg aagcggccct gcaagacggc gttgtcgagc ggattcgtca
     4381 ccatgtgcaa gccggcgcgc ttgatgttgt tgacgaggcg gtacgcatac gcggcgttgt
     4441 acgaatgcat cgcggtggta tgactcgcag tgacgcgacc cgacatgccg cggcgaatgg
     4501 tctcggcggc catcacttcc acaaagcgcg aatggtcgtc gtcggtctcg tcgcaatgaa
     4561 tatcgacccg cagaccgaat tcctccgcca ggtcgaaggt gaacttcacc gactcgacgc
     4621 cgtcttcgcg ggtgagctcg tagtgcggaa tgccgccgac aacatcggca ccgagttcga
     4681 cggctttgcg catgagttcg cggccgccgt cgaacgacat gatgccgagc tggggaaagg
     4741 cgacgagctg gagatcgacc tggccgcgcg cctcttcgcg gatctcgaca agcgcccgca
     4801 atgccgtgag attcgagtcg catacatcga cgtgacttcg cacgtgctga accccgttgg
     4861 ccaactgcca gcgcaacact tcgcgcacgc ggctcttcac gtcgtcgacg gtcagatcct
     4921 tgacgcgctc gctccatacg gcgatgcctt cgaacagcga gcccgatttg ttgggtcgcg
     4981 gctggccaac ggtgagcact gcgtcgaggt ggatatgcgg ctcgaccagg ggcggcgtga
     5041 cgagcgcgcc cgcgacgtcg atcgattcgc gagcctcagg ctcaggtgcc acgaagatgc
     5101 cgtcgcgcac cccgagatcg gtcagcccgt ctgacccgcg cactcgcgcg ttacgcagca
     5161 gtaggtcgag cacgcgcaga acctatcagc cgacgaagcg ccgagcgccg ctgtattcgc
     5221 tcgcgaacgg ctgggccgaa agcgacgaat actcgaccgg gctccccgtt tgcaacgagc
     5281 tcagcatcgt gtcgttgccg acgtagattc cgacgtgatg cacgaccccg ttcgacgcga
     5341 agaacaccag atcacccggc cgcagatcgg cgcgatcgac agcctcaccg acccttgcct
     5401 gagcatcggc gtcgcgcgga atgatcagac cgcgcatggc gaagacgagg tgcgtgagtc
     5461 ctgagcagtc gaagccgaat cccgatgtgc caccccacag gtagggcaat ccgagaaaga
     5521 gcttggccgt acggacgatg tcatctgcag tgggcgcaag cggcccggct ccgggtgaca
     5581 cgagcgcgac gtcggccgcg cgcaaggaca cggcacggcc gtcaggcagc tgcgattgca
     5641 cccaccgcgt gttgtctgac agcaccggca atcgggtcgc catgctcaca tcgacaacct
     5701 gcgtcacgtc gtcgttggcg taaatccacg cgctcggtga ggtaacgacg agctcttgac
     5761 tggttgcagt cggcgtccgg aaagtcagct gtgcaaggtg aatccaaccc ggatacccac
     5821 tcgcgtcgag cggcgtcggt tgatcggcga ccacgacgtg cgcccaggtg ccctgtcgct
     5881 gatcaacgac cacctgctcg ccgagaagca gttggctgtc gacgcgaccg ttcagatcga
     5941 gtcgttgcgc cgtactcata gaactcaacc atgcctggac gccggcgggc tggcgtaact
     6001 ccgcagcgtc gaccgatcgt ggtgacgaag ggctgtgcca cagcgtggcg accgacacgt
     6061 cgaccaccgc ctgcgcagga agagcgggaa gcgcggggtt cgccggctgt tgggtcgcat
     6121 gaactgactg ggtcgagcca gggtgaatcg gacctgccgc cgttgtcgtc ggccggtggg
     6181 tcttgccacc ttgtggggcc agtgcgtacg gcgacgatgc ccccgaaggc ttgccgcccg
     6241 gcgtttcgag ctgggctgcc atgagtgacg aagccggagc cgagtacgcc cctgacggtg
     6301 ctcccacctg cgcactcgac agcgacgtac tcggcgccag cgcaaagccc tgcggtgggc
     6361 gcgagtcttg tgacctgttc gacttggtgg agcagccagc gagcatcccc agtgttacca
     6421 ggacggccgt gaccgccccg gctgatcgcc gtcccctcat gc
//
LOCUS       DASWVF010000807         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_23113, whole genome shotgun sequence.
ACCESSION   DASWVF010000807 DASWVF010000000
VERSION     DASWVF010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23113"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGD55_14315"
     CDS             <1..231
                     /locus_tag="VGD55_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871568.1"
                     /translation="GSVILFAIQYRFADSGAWYLIGLGATAVAFALFLPKGIWGTVQG
                     RFHTELLPIGHRLTEQTAVGASLPEAGASGGD"
     gene            221..1711
                     /locus_tag="VGD55_14320"
     CDS             221..1711
                     /locus_tag="VGD55_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0871569.1"
                     /translation="MATDAKALRIPTIPVFEQFIGGAWGPAWSGRTFDDLDPWSGQIV
                     ARMAAGDAWDARRAIEAAQEAFASWGTSTPSTRQNIFLRAADILERRRAEVMDWLASE
                     TGCGHDFGLIQIEFVLSLLKQASGAGYAAVGQVIPSDQPGSLAMAVRRPVGVVAAIVP
                     WNAALILSARAIVAPLMLGNTVVLKPSEESPWVAGVLWGQIFQEAGLPPGVLNVVTHA
                     PGDAGAIGNELIEHPFVRRINFTGSTATGRRLAEKAGRHLKRMVLELGGQNPLIILSD
                     ADLAYAVDAAAYGAFLHQGQICMCARRLFVERPVADEFLERFSAKSARLAHGDPRDAR
                     TVIGPLINNHALSMVTRRVDEAVAGGARVLAGGHPVGRCYPATVITDVPSNCELSQEE
                     TFGPVAIVDIVDDADDAIRRANDSVWGLTAGVITGDAERGMAIAERLDAGIIHINDQP
                     IHDEPQMPFGGVKDSGWGRFGVTFAPEEFTDTHWVTVKRRQRTFPF"
     gene            complement(1831..>3504)
                     /locus_tag="VGD55_14325"
     CDS             complement(1831..>3504)
                     /locus_tag="VGD55_14325"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0871570.1"
                     /translation="ATVRIGGALGTGIGGQVTKDLAVAESIAVPLTMILLMVVFGSVV
                     AALLPLSIAILAIFGTLAELNVLTHVTSVSIFAINLTTALGLGLGIDYALLMVSRFRE
                     ELAGGAEVAEAVARTTATAGRTIAFSALTVAAALSAMIVFPVYFLKSFAYAGVGVTAF
                     AAIGALIVMPALLAVLGHRVNAGKIPGIKAVRDPETPFWNRLANGVMRRPIIAAAPVV
                     LVLLFLATPLLKVTFAAPDDRALPTSAASHQVGDALRNDFATAPSVTDVVISPPLASA
                     ALSTYESALSAVPGVQHVVDAGTVTGTQRLAITTGLYSTSSAAATMVKQIRDVAVPAA
                     SQVLVGGDTAALIDGKYAISSSLLLAGGIIVATTFILLFLFTGSIVQPVRALLGNALT
                     LGATLGAMVWIFQDGHFSKLLGFTPTPTNTSMPVLLFCIAFGLSMDYEVFLMSRIKEL
                     HDGGATNADAVTHGLARTGRIVSTAAALLAISFFAFATSKVSFIQFFGLGTGFAILID
                     ATLVRGVLVPAFMRVFGENSWYAPAPLRKLHHRIGLSDAEPPALELAGA"
BASE COUNT          605 a         1114 c         1204 g          581 t
ORIGIN      
        1 ggttccgtta tcttgttcgc gatccagtac cggttcgccg acagcggcgc gtggtatttg
       61 atcggcctcg gtgcgacggc ggtggcattc gcgctgttcc tgccgaaggg catctggggc
      121 accgtgcaag ggcgctttca caccgagctg ctgccgatcg gccaccgttt gactgagcaa
      181 acagcggtgg gcgcgagttt gccggaagcc ggtgcgagcg gtggcgactg acgcgaaggc
      241 cttgcggatc ccgacgatcc cggtcttcga gcagttcatc ggcggtgctt gggggccggc
      301 gtggagtggg cgaacgttcg acgacctcga cccgtggagt ggacagatcg tcgctcgcat
      361 ggcagccggc gatgcgtggg atgcgcggcg ggcaatcgaa gcagcgcagg aggcgttcgc
      421 cagttggggg acgtcgaccc cgagcacccg ccagaacatc ttccttcgag cagccgacat
      481 cctcgagcgc cgccgcgcag aggtgatgga ttggctcgcg tcagaaacgg gctgcggcca
      541 cgacttcggc ctgatccaga tcgagttcgt cctttcgctg ttgaagcaag cctcaggtgc
      601 cggctatgcg gccgtcggcc aggtgatccc gtcggatcaa ccaggttcgc tcgcgatggc
      661 agtgcgccgg ccagtcggcg tcgtcgcagc gatcgtgccg tggaacgccg cgctgatcct
      721 gtcggcgcgc gcgatcgtgg ctccgttgat gctcggcaac actgtcgtgc tgaagccgtc
      781 ggaggaatcc ccttgggtcg ccggcgtgct gtgggggcag atcttccagg aggccggcct
      841 accccccggc gtcctcaatg ttgtgacgca tgcacccggt gatgccggcg ccatcggcaa
      901 tgagctgatc gagcatccgt ttgtccgccg catcaacttc accggctcaa ccgcgacggg
      961 tcggcgactc gcggagaagg cgggtcgcca cctcaagcgg atggtgctcg aactcggcgg
     1021 ccagaacccg ctcatcattt tgtcggacgc cgatctggcg tacgcagtcg atgctgccgc
     1081 ctacggcgcc ttcttgcacc aggggcagat ctgcatgtgc gcgcgccggc tcttcgtcga
     1141 gcggccggtt gccgacgagt tcctcgagag gttctcggcg aagtcggcgc gcctggccca
     1201 cggtgacccg cgcgatgcgc gcaccgtgat cgggccgctg atcaacaatc acgcgctgtc
     1261 gatggtcacc cgccgggtag atgaggcagt tgctggcggc gcgcgggtgc ttgcgggcgg
     1321 ccacccggtg ggccgctgct atcccgcgac ggtcatcacc gacgtcccgt cgaattgtga
     1381 gttgtcgcag gaagagacgt tcggacctgt cgcaatcgtc gacatcgtcg atgacgcaga
     1441 cgacgcgatt cggcgggcga acgactcggt ctggggtctg acggccggtg tgattacggg
     1501 tgacgccgaa cgcggcatgg cgatcgctga acgtctcgac gccggcatca tccacatcaa
     1561 cgaccagccg atccacgacg aaccgcagat gccgttcggt ggcgtgaaag acagcggctg
     1621 gggccgcttc ggagtcacgt tcgctcctga ggaattcacc gacacgcact gggtcaccgt
     1681 gaagcggcgg cagcgcacct tccccttcta gcccatcgcc ccaaccccta cccgctactt
     1741 gacatcccgt gtggttgtga aagcgccctt attcggtctg tttcacgacc acacgggatg
     1801 tgaggtgccg atctggcaag cgacctcgtc ctacgcgccg gcgagttcta gtgccggagg
     1861 ctcggcgtcg ctcagaccga tccggtggtg caacttgcgc agcggagccg gcgcgtacca
     1921 gctgttctcg ccgaacaccc gcatgaaggc cggcaccagc acgccgcgca ccaaggtcgc
     1981 gtcgatcagg atcgcgaagc cggtgcccag gccgaagaac tggatgaagc tgaccttgct
     2041 cgtggcgaag gcgaagaagc tgatcgcgag cagggccgcg gcggtcgaga cgatccggcc
     2101 ggtgcgggcg aggccgtgag tcaccgcgtc ggcgttggtt gccccgccgt cgtgtagctc
     2161 cttgatgcgg ctcatcagga acacctcgta gtccatagag agaccgaacg cgatgcagaa
     2221 caacagcacc ggcatcgagg tgttggtcgg agtcggcgtg aacccgagca gcttcgagaa
     2281 gtggccgtcc tggaagatcc acaccatcgc gccgagtgtg gcaccgagcg tgagcgcgtt
     2341 gccgagaagc gcacgcaccg gctgcacgat gctgccggtg aacaggaaca acaagatgaa
     2401 cgtcgtggcg acgatgatgc cgccggcgag cagcaggctc gagctgatcg cgtacttgcc
     2461 gtcgatcagt gcagccgtgt cgcccccgac gagcacctgc gaagccgccg gcactgcgac
     2521 gtcacggatc tgcttgacca tcgtcgccgc tgcactcgac gtgctgtaga gaccggtcgt
     2581 gatcgcgagt cgctgcgtac ctgtcacggt gccggcgtcg acgacgtgtt gcacgccggg
     2641 caccgcgctc agtgcgctct cgtaggtcga cagcgccgcg gacgccagcg gtggggagat
     2701 cacgacgtcc gtgacactag gagccgtggc gaagtcgttg cgcaaggcgt cgccgacctg
     2761 gtggctagcg gcgctggtcg gcagcgcgcg gtcatcgggt gccgcaaacg tgaccttcag
     2821 gagcggtgtc gccaggaaca acagcacgag gacgacgggc gccgcggcga tgatcggacg
     2881 acgcatcacg ccgttggcca gccggttcca gaacggggtc tcggggtcgc gcactgcctt
     2941 gatcccgggg atctttccgg cgttgacccg atgaccgagc acggcgagca gggcaggcat
     3001 cacgatcagc gcgccgatgg cggcgaacgc ggtgacaccg acgcctgcgt aagcgaagga
     3061 cttcaggaag tacaccggga acacgatcat cgccgacaac gcggcggcca ccgtcaacgc
     3121 agagaaagcg atcgtgcgac ccgcagtggc cgtcgtccgc gctactgctt ctgcgacttc
     3181 agcgccgccg gccagctctt cacggaaccg gctcaccatc aacaacgcgt agtcgatacc
     3241 gaggccgagg ccgagagcgg tggtgaggtt gatggcgaag atgctgacgc tcgtgacgtg
     3301 tgtgagcacg ttgagttcgg ccagcgttcc gaagatggcg aggatcgcga tgctcagcgg
     3361 cagcaacgcg gcgaccacgc tgccgaacac gaccatgagc aagatcatgg tgagtggcac
     3421 ggcgatgctt tccgccaccg cgaggtcctt ggtgacctgc ccgccgatgc cggtgccgag
     3481 cgcgccgccg atacgaaccg tggc
//
LOCUS       DASWVF010000808         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_73271, whole genome shotgun sequence.
ACCESSION   DASWVF010000808 DASWVF010000000
VERSION     DASWVF010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73271"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1246
                     /gene="panB"
                     /locus_tag="VGD55_14330"
     CDS             443..1246
                     /gene="panB"
                     /locus_tag="VGD55_14330"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0871571.1"
                     /translation="MTNSRLGVLELQKRKDDGRRWAMLTSYDQLTAGIFEAAGIDVLL
                     VGDSAANTVLGYPDTVSVTVDELIPLVRAVVRGTSTALVVADLPFGSYQVSPQQALET
                     SVRFMKEAGAHAVKFEGGAHIAEHARTVVAAGIPVMGHVGFTPQSVYRLGGYRVQGRG
                     DAAEQVVEDALALQSAGVFAVVLEMIPADLAKRVTAELSIPTIGIGAGPHCDAQVLVW
                     TDMAGLADGHAPKFVKPYADLRGVLLQAAKQYADDVLSGDYPDSEHSYN"
     gene            complement(1256..1954)
                     /gene="npdG"
                     /locus_tag="VGD55_14335"
     CDS             complement(1256..1954)
                     /gene="npdG"
                     /locus_tag="VGD55_14335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY0871572.1"
                     /translation="MTDSPAPSPAPVPTASPAPAFGTIGILGGTGDQGRGLAYRWALA
                     GLDVVIGSRDAARAAVAASEIAAGVRGATNHDVGVQADIVVVAVPWDGHAELLRSLSV
                     VLAGKIVVDCVNPLGFDERGAYALEVQEGSAAQQAAALLPESRVVAAFHHLSSVLLND
                     RAVVDVDCDIMVLGDDRGATDIVQLLAATLAGARGFYGGRLRNAHQVEALTANLISVN
                     RRYRTRAGVRLTGV"
     gene            2058..2690
                     /locus_tag="VGD55_14340"
     CDS             2058..2690
                     /locus_tag="VGD55_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871573.1"
                     /translation="MDKARIALMRDLLAGTGWVERTRSFGHALRRAPHDVGGLLVVGT
                     PTEDPWHFAAHLDDEARWSDLPTLAPVLVRWAPPPNAPAHLAIGLQRLEQVGRHETVF
                     VVAPDAAPVPLLERVADARKAGATVFALDAGDPDLEGLAHESLWVPQGTTDGIVVPEF
                     DLVSHLVSAAAGETGDSSTGRVAALRRRLGRLLDAVSDSDDADTPTNQRP"
BASE COUNT          532 a          932 c          894 g          463 t
ORIGIN      
        1 gcgagggtct tcagcgccac gttaagaacc gagttgtcga gcaccacgat cagcaggcag
       61 agcaccatca cggccaggat cgcccagcgc cggttgtaga tcttgctgtc gaccgcggta
      121 agcgcgggag atgagctggt catgcgggcc tctttcgata cgcgtccgtc ttgtattcga
      181 ggaccataac ctgagcgagt tcagatacgc aactgtctcg tatcgaaaac gttcccaacc
      241 ccgcttccag cctccaacca accctccaac caaccctcca accaaccctc caaccaacag
      301 agcggacgtt tactgccctc caggggcagt aaacgtccgc tgtgtcgtca agagacgcga
      361 ccccggcgcc gagaaggtgc gaagatgaaa gtgcccgggt acgccgaacg gcgactcgag
      421 gcctgaaagg tcggctctcg ctatgactaa ctcgcgtctc ggcgtcctcg aactccaaaa
      481 gcgcaaggac gacggccggc ggtgggcgat gctcacctcc tacgaccaac tgacggctgg
      541 catcttcgag gcggccggga tcgacgtcct gctggtcggt gattcggccg cgaacaccgt
      601 cctcggctat cccgacacgg tctcggtgac cgtcgatgag ctgatccccc tcgtgcgcgc
      661 agtcgtacgg ggcacgagca ccgcgctcgt cgtcgccgac ttgccgttcg gctcctacca
      721 ggtctcgccc cagcaggcgc tcgaaacctc tgtgcgattc atgaaggagg cgggcgcgca
      781 cgcggtgaaa ttcgagggcg gggcgcacat cgccgagcac gcacggacgg tggtggccgc
      841 cggcatcccg gtgatggggc acgtgggctt cactccccaa agcgtgtatc gcctcggcgg
      901 ctatcgcgtg cagggtcgcg gtgacgccgc cgaacaggtc gtcgaagatg cgctggcact
      961 gcaatcagcg ggtgtattcg cggtggtcct ggagatgatt ccggccgacc tcgcaaagcg
     1021 ggtaactgcc gagctttcca ttccgactat cggtatcgga gcgggtccgc attgcgatgc
     1081 ccaggtgctc gtctggacag acatggcagg cctagcagac ggtcacgcgc ccaagttcgt
     1141 aaagccgtac gccgacctgc gcggggttct tcttcaagcg gcaaaacagt atgcggacga
     1201 cgtgttgtcg ggcgattacc cagactccga acactcctac aactaagacg cagcttcaga
     1261 cgcccgtcag ccgcacgcca gcgcgggtgc gatatcggcg atttaccgaa atcaggttcg
     1321 cagtgagcgc ttcgacctga tgtgcattgc gtaaccggcc gccatagaaa ccgcgagcgc
     1381 cagcgagtgt ggcggcgagc aactgcacaa tgtcggtcgc gccccgatcg tcgccgagaa
     1441 ccatgatgtc gcaatcgaca tcgaccaccg cgcgatcgtt cagcagcacc gacgacagat
     1501 ggtgaaacgc ggccacgaca cgactctcgg gcagcaatgc cgccgcctgt tgcgccgcgc
     1561 ttccttcttg gacttcgagt gcatacgcgc cgcgttcgtc aaagccgagc gggttcacac
     1621 agtcgacgac aatctttccc gcgaggacga cggagagcga ccgtagcaac tctgcgtgac
     1681 cgtcccacgg cacggcgaca acaacgatgt ctgcctgcac accgacgtca tgattggtgg
     1741 cgccgcgcac gcccgctgca atctccgacg cggcaacggc ggcacgtgca gcatcgcgcg
     1801 atccgatgac gacatcgaga ccggcgagcg cccaccggta ggcaaggccg cgaccctgat
     1861 caccggtgcc gccgagaatg ccgatggtgc cgaacgcagg tgcaggtgac gcggtaggta
     1921 cgggtgcagg cgagggtgca ggtgagtcgg tcatggcctg atcctcgcag ttgcgcgacc
     1981 atacttgggc aggtgcacgc ggtcacgccg cgagcgatac atgaagtcgg tcggacgtcg
     2041 gtcgagtgga ggtgcgcatg gacaaggctc gtatcgcact catgcgcgac ctcctcgcgg
     2101 gcaccggctg ggtcgaacgc acgaggtcgt tcggtcacgc gctgcgacga gcaccccatg
     2161 acgttggcgg gttacttgtc gtcggcactc cgaccgagga tccgtggcat ttcgcggccc
     2221 acctcgacga cgaggcgcgc tggtcggatc ttccaacgct ggcaccggta ctggtccgat
     2281 gggcaccgcc gcccaacgcg cccgcgcatc tcgcgatcgg actccagcgt ctcgaacagg
     2341 tgggtcgtca cgagacggtg ttcgtcgtgg cccctgacgc tgctcccgta ccgctgctcg
     2401 aacgcgtcgc cgatgcccgc aaagcgggtg ccacggtgtt tgcgctggac gccggtgatc
     2461 ccgacctcga gggtctcgcg cacgagtcgt tgtgggttcc gcaaggcact accgacggga
     2521 tcgtcgttcc ggagttcgac ctggtgtcgc acctggtgag cgcggcagcg ggtgagactg
     2581 gcgacagctc gaccggtcgc gtcgcagcgc tacggcgacg acttgggcgg ctcctcgatg
     2641 cggtcagcga ctctgacgac gccgatacac caaccaacca acgaccatga cgacgacgac
     2701 gccgagcacg gcccaaccga tcgtgctgat gtcggccgcg acccgatgcc aggcgttgcc
     2761 cgcaagataa cccgcgatca ccacggccgt cgcccagacc gtaccgccgg caaagttcca
     2821 g
//
LOCUS       DASWVF010000809         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_328230, whole genome shotgun sequence.
ACCESSION   DASWVF010000809 DASWVF010000000
VERSION     DASWVF010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328230"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGD55_14345"
     CDS             complement(<1..304)
                     /locus_tag="VGD55_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871574.1"
                     /translation="MFIQIIQGTVTDADALQRSVARWRKEIKPGAHGYLGSTGGVTPD
                     GRGITLVRFESEADAQANSDRAEQGAWWNEASKAFGDDVTFHNCAEVESILGGGNDA"
     gene            complement(387..1406)
                     /locus_tag="VGD55_14350"
     CDS             complement(387..1406)
                     /locus_tag="VGD55_14350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0871575.1"
                     /translation="MKRIGFLSFGHWTPSPHSQTRSASDALLQSIDLAVAAEELGADG
                     AYFRVHHFANQLASPFPLLAAVGARTSRIEIGTAVIDMRYENPQYMAEDAGAADLISG
                     GRLQLGISRGSPEQVIDGFRYFGYVPAEGTDAADMAREHARVFLEVLGGEGFAKPNPR
                     PMFPNPPGLLRVEPHSEGLRDRIWWGAGTRKTAEWTAEQGMNLMSSTLLSEDTGVPFH
                     QLQAEQIERFRKTWADAGHARVPRVSVSRSIFPIVNDVDRAYFGREGNSQDQVGYLDG
                     GTARFGKTYAGDPQKLIEQLAQDEAIAAADTLLLTIPNQLGVDYCAHVLDSVLRHVAP
                     ELGWR"
     gene            complement(1526..>2276)
                     /locus_tag="VGD55_14355"
     CDS             complement(1526..>2276)
                     /locus_tag="VGD55_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY0871576.1"
                     /translation="RDGCAPAEALERVAQICLASLADAGNPPRDLRGHEHAAVVIHLD
                     AARVPAHEAPARSAERDPAGPIGARPYARVAGGPGLPDRVIQRLLCDGRIRTVIHDGP
                     NVLDVGRSHRLVTDRQYRALLLRHHNHCAHPGCPNAKNLHAHHRIHWIHGGRTDLDNL
                     LLLCERHHLAHHGGEFEVFTLGAGRFRFVTSDGRDLSAPHRNPASPNTPPLEDEHAHV
                     EADAATPRWDGQRLDRPYAISVLAQRRQIAS"
BASE COUNT          348 a          711 c          809 g          408 t
ORIGIN      
        1 cggcgtcgtt gccgccgccg agaatcgatt ccacttcggc gcagttgtgg aaggtcacgt
       61 cgtcgccgaa tgcctttgac gcttcgttcc accacgcacc ttgctcggcg cgatcgctgt
      121 tggcttgcgc gtcagcctca gactcgaaac ggaccagcgt gatgcctcgg ccgtcgggag
      181 tgaccccgcc ggtggaaccc aagtaaccgt gcgcgccagg cttgatctcc ttgcgccacc
      241 gagccactga tcgctgcaga gcgtcagcat cggtcaccgt gccttggatg atctggataa
      301 acatgtgcgc cacctttcag agggtgagcc gaggctatgc gttacccgcg cggcgcacta
      361 ggggtcagag gcctcggccg agcggcttaa cgccagccaa gctccggcgc gacgtgccgc
      421 agaaccgagt cgaggacgtg tgcgcagtag tcgactccaa gctggttggg aattgtcagc
      481 agcagcgtgt cggcagccgc gatggcctca tcctgggcga gttgctcgat gagcttctgg
      541 gggtcgccgg catacgtctt gccgaagcgc gccgtgccgc cgtcgaggta gccgacttgg
      601 tcctgcgagt tgccttcgcg gccgaagtac gcgcggtcga catcgttgac gatcgggaag
      661 atgctgcggc tgactgacac gcgtggcacg cgggcgtggc cggcgtcggc ccaggtctta
      721 cggaaccgct cgatctgctc agcttggagc tgatggaacg gcaccccggt gtcctcgctc
      781 agcagtgtcg aactcatcaa gttcatcccc tgctcggcag tccactcagc ggttttccgg
      841 gtgccggcgc cccaccagat gcggtcgcgc aaaccctcgg agtgcggctc gacgcgcagc
      901 agacccggcg ggttggggaa catcggccgc gggttcggct tggcgaaacc ctccccgccc
      961 aagacctcga ggaagacccg cgcgtgttca cgggccatat cggctgcgtc ggtgccttcg
     1021 gcaggcacgt agccgaagta gcggaaaccg tcgatgactt gctcgggtga cccgcggctt
     1081 atcccgagct ggagccgccc gcccgagatg agatcagcgg cgccggcatc ctcggccatg
     1141 tactgcggat tctcgtaccg catgtcgatg actgcagtgc cgatctcgat cctgctggtc
     1201 ctggcgccga ctgccgcgag gagcgggaag ggcgaggcca gctgattggc gaaatggtgc
     1261 acccggaagt acgcgccgtc cgcgccgagt tcctcggcgg caactgcaag gtcaatggac
     1321 tgcagcaacg cgtccgaggc cgagcgagtc tgcgagtgcg gcgacggcgt ccaatgaccg
     1381 aacgacaaga acccaatgcg tttcatgacc gaccaaacat ccctttagtc gctgctcttc
     1441 ccgtcaacaa gtgaaccacg ccaggtcatg tctaacgtcg ccgctgacag cgcatcaggt
     1501 ctcgctgcca gctacggctg tgctgtcagc tggcgatttg tcggcgttgg gcgaggacgc
     1561 tgatggcgta ggggcggtcg agtcgttggc cgtcccatcg gggggtggcg gcatcagctt
     1621 ctacgtgtgc gtgttcgtct tcaagcggcg gggtgtttgg gcttgccggg tttcgatggg
     1681 gtgcgctgag gtcgcggccg tcggaggtga cgaaacggaa cctgcccgcg ccgagggtga
     1741 acacttcgaa ctcaccgccg tggtgggcga ggtggtggcg ttcgcatagg agtagcaggt
     1801 tgtctaggtc ggtgcggccg ccgtggatcc agtggatccg gtgatgggcg tgcaggttct
     1861 tcgcattcgg gcagccgggg tgggcgcaat ggttgtggtg gcgtagcagc agggcgcggt
     1921 attggcggtc ggtgaccagg cggtgggatc ggccgacgtc gagcacgttg ggcccgtcgt
     1981 ggatcacggt gcggatccgg ccgtcgcaca gcagccgttg gatgacccgg tcgggaaggc
     2041 ccggcccacc cgcgacccgc gcgtagggcc gcgcaccgat cggcccggcc gggtcgcgtt
     2101 cagctgagcg tgccggcgcc tcgtgagccg gtacccgcgc ggcgtcgagg tggatgacca
     2161 cggcggcgtg ttcgtgcccg cgcaggtcgc gcggcgggtt gcctgcatcg gccaggctcg
     2221 ccaggcagat ctgcgcgacg cgctccagtg cttccgcggg ggcgcagccg tcgcgt
//
LOCUS       DASWVF010000810         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_507333, whole genome shotgun sequence.
ACCESSION   DASWVF010000810 DASWVF010000000
VERSION     DASWVF010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507333"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..970)
                     /locus_tag="VGD55_14360"
     CDS             complement(11..970)
                     /locus_tag="VGD55_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0871577.1"
                     /translation="MTTDYAIEATALTKRFGSTQALAGVDFAVREGTVLGVLGPNGAG
                     KTTAVRILATLLQADSGTARVGGFDVACDPEKVRQIVGLTGQYASVDEDLTGTQNLVL
                     IGQLLDLRTKDARARAAELLDWFDLGDAADRRAKTYSGGMRRRLDLAASLVGRPAVIF
                     LDEPTTGLDPAKREDMWDVVRNLVTEGSTVLLTTQYLDEADALADEITVIDHGKVIAH
                     DTPEGLKRVVGGQTLTVRAADSSRLPEVTAIVAQVAAGKPELSARGVVSASVDGDAAL
                     TATVARLAQAGIAVTELSLHLPSLDEVFFSLTGTRHLDTEDVA"
     gene            complement(1055..1483)
                     /locus_tag="VGD55_14365"
     CDS             complement(1055..1483)
                     /locus_tag="VGD55_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0871578.1"
                     /translation="MTTSVTYDDVEVGEQLPTRSFPITRADLVRYAGASGDFNPIHWN
                     APFAVSVGLPDVIAHGMLTMAETVRVVTDWIGDPAAVVDYSVRFTRPVVVPDDAAGAT
                     LHVEGVVAEKLGEGLARIDLTARFDGEKVLGMARATVRLS"
     gene            complement(1480..>1664)
                     /locus_tag="VGD55_14370"
     CDS             complement(1480..>1664)
                     /locus_tag="VGD55_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0871579.1"
                     /translation="YARPIFAGDELSVTMTVAEIRTLAGNDIITTRGDIATTDGDAVC
                     TVFSTLVSRAPDESAP"
BASE COUNT          256 a          538 c          594 g          276 t
ORIGIN      
        1 atggtgctgc tcatgcgacg tcctccgtgt cgaggtggcg ggtgccggtg aggctgaaga
       61 agacctcgtc gaggcttggg agatgaaggg acaactcggt gacggcgatc cccgcttgcg
      121 cgagcctggc gacggtcgcg gtcaatgcgg catcgccgtc aaccgacgcg ctcacaaccc
      181 cgcgggccga gagttcaggc ttgccggcgg cgacctgcgc gacgatcgcc gtgacctcgg
      241 gcagccgcga actgtcggcc gctctgaccg tcagcgtctg accgccgacg acacgcttca
      301 gtccttctgg ggtgtcgtgg gcgatcacct tgccgtggtc gatgacggtg atctcgtctg
      361 cgagcgcatc ggcttcgtcg aggtactggg tggtgagcag gaccgtcgag ccctcggtga
      421 cgaggttgcg cacgacgtcc cacatgtcct cgcgcttcgc cggatcgagc ccggtcgtcg
      481 gttcgtccag gaagatcacc gcaggccgtc cgaccaggga ggctgcaagg tcgagacgac
      541 ggcgcatgcc gccggagtag gtcttcgcgc gccggtctgc agcgtcgccg agatcgaacc
      601 agtcgagcag ttcggcggct cgcgccctgg cgtccttggt ccgcaagtcg agcagttgcc
      661 cgatcagcac caggttctga gtgcccgtca ggtcctcgtc gaccgaggcg tactggccgg
      721 tcaggccgac gatctggcgc accttctcgg ggtcgcaggc cacgtcgaat ccgccgactc
      781 gtgcggtgcc ggagtcggct tggagcaagg tggcgaggat tcgcacggcg gtggtcttgc
      841 cggccccgtt cgggccgagc acgccgagga cggtgccttc gcgcacggcg aagtcgacgc
      901 cggcgagggc ttgggtggat ccgaagcgct tcgtgagcgc ggttgcttcg atcgcgtagt
      961 cagtggtcat gggaagacga tgacccgggc cgctggcacc gagcccgcac ggcgctggca
     1021 cggcgctggc acgccgttgg cacgccgagc tagctcagct cagccggact gtcgcccttg
     1081 ccatgccaag caccttctcg ccgtcgaagc gcgccgtcag gtcgatgcgc gcgagaccct
     1141 cgccaagctt ttcggcgacg acaccctcga cgtgcagcgt tgctccagcc gcgtcgtcgg
     1201 gcacgacgac gggcctggtg aaacgaacgg aatagtcgac gacggcggcc gggtcaccga
     1261 tccagtcggt gaccacgcgg acggtttcgg ccatggtgag catgccgtgg gcgatcacgt
     1321 cgggaagccc gacgctcacc gcgaagggcg cgttccagtg gatcgggttg aagtcacccg
     1381 acgccccggc gtagcgcacc aggtcggccc gggtaatcgg gaagcttctg gtgggcagct
     1441 gctcaccgac ctcgacgtcg tcgtaggtca ccgaggtcgt catggcgcac tctcgtcggg
     1501 agcgcgcgac acgagggtcg agaacacggt gcaaaccgcg tcgccatcgg ttgtcgcaat
     1561 atcgccgcgg gtagtgatga tgtcgttgcc cgccagcgtg cgaatctcgg cgacggtcat
     1621 cgtgacggac agctcgtcgc ccgcgaagat cggccgggcg tagc
//
LOCUS       DASWVF010000811         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_379729, whole genome shotgun sequence.
ACCESSION   DASWVF010000811 DASWVF010000000
VERSION     DASWVF010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379729"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1029)
                     /locus_tag="VGD55_14375"
     CDS             complement(238..1029)
                     /locus_tag="VGD55_14375"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0871580.1"
                     /translation="MTTRQRFGGKVVVVTGAGRGIGLGIAKRFAGEGAAVVLADRDPA
                     VVQTAKMLSHAGANCLGVAADVISEADVASLFVTVADQFGGVDVLINNAGTITISKLE
                     ELSLADWNRVLAVNVTGAFLCAKAAATLMRAAGRGGRILNAASGQARQGFIYTPHYAA
                     SKFAVVGLTQSLAKELAKDAVTVNAYAPGIVGGAMWDYNDREWGRLLGEYQPGELMAE
                     WVAGIPLGRAGTDDDIAELLLFLASDQAGYITGQTINVDGGMFMS"
     gene            complement(1026..>1705)
                     /locus_tag="VGD55_14380"
     CDS             complement(1026..>1705)
                     /locus_tag="VGD55_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871581.1"
                     /translation="GLHGEIARLPWTVTAVDDVSITARVNTVSPSWQVKRTVAMTDVG
                     FTLHTEAVNTSEASLLCSYGEHPCFARDVFAGGRIDLAASAAFVPTPQEDPVRAKFLP
                     TGAIAWPRLATRDGSWSDADRIPEVADGRLDHVSLTLAAPRISLYAPRLDGHLDLVVQ
                     LQELPHALLWERFGRSAKQWNADVFAVEPSSAPGRTVDDAVSAGALRSVAPGQSVTFG
                     TEVEWSR"
BASE COUNT          311 a          566 c          537 g          291 t
ORIGIN      
        1 tgcgtcaaga tcggtggtca acatggaagc gcgccgttgt gggtcgtcat ggtcaaggac
       61 tcctctcact gaatcgcttg cggcgtgctt aatttgcggc gacgtcggtc caggaccacc
      121 gagccggcaa cgtggatggc aacagctgcg cgcagcgggt atcacgttgc cgatacgggc
      181 gaacctccct cggtgcactt cttaggtacg gagtgcgtgg agcgggcccc tgctgagtca
      241 gctcatgaac atgccgccgt cgacgttgat cgtctgtccc gtgatgtagc cagcttgatc
      301 cgaggcgagg aacagcagca gctccgcgat gtcgtcgtcg gtacccgccc gacccagcgg
      361 aatgccggcg acccattcgg ccatcagttc gccgggctgg tactcgccga gcagtcgtcc
      421 ccactcgcgg tcgttgtagt cccacatggc gccgccaaca attcccggcg cgtaggcgtt
      481 gacggtgacc gcgtccttcg ccaactcctt ggccaggctc tgggtgaggc cgacaacggc
      541 gaacttgctg gccgcatagt ggggggtgta gatgaaccct tgccgtgcct ggcccgacgc
      601 ggcattgagg atccgtccgc cgcgaccggc ggcccgcatt agcgtcgctg ccgccttcgc
      661 gcataggaac gcgccggtga cgttgaccgc gagcacccgg ttccagtcgg ctagcgagag
      721 ttcctcgagc ttcgagatgg tgatcgtgcc cgcgttgttg atgagcacat cgacgccgcc
      781 gaactggtct gcgacggtca cgaacagcga ggcaacgtcg gcttcggaga tcacatcggc
      841 agcgacgccc aggcagttgg cgcccgcgtg cgacagcatc ttggcggtct gtacgaccgc
      901 ggggtcgcgg tcggcgagca ccacggcggc cccttcgcca gcgaaccgtt tggcgatgcc
      961 cagcccgatc ccgcggccag ctccggtgac gacgacgacc ttgccaccga accgctggcg
     1021 tgtcgtcatc ttgaccactc gacttcagtc ccaaacgtca cggactggcc gggagcaacc
     1081 gagcgcaaag caccagccga cactgcgtcg tcaaccgttc gaccgggggc cgaactcggc
     1141 tccacagcaa atacatccgc gttccactgt ttggcagaac gaccaaatct ttcccacaag
     1201 agtgcgtgcg gcagttcctg aagctgaacg acgagatcga gatgaccgtc taaccgcggc
     1261 gcatacaacg agatccgcgg agccgccaaa gtgagcgaga cgtgatcgag tcggccatcg
     1321 gcgacctccg ggatgcggtc ggcgtccgac cacgacccgt cgcgagtcgc aagacgcggc
     1381 cacgcaatcg cgccggtcgg caagaacttt gccctcaccg gatcctcctg cggcgtcggc
     1441 acaaacgctg cgctcgccgc caggtcgata cgacctcctg cgaacacatc acgtgcgaaa
     1501 caaggatgct caccgtaaga gcacagcagc gacgcctcgc tcgtgttcac ggcttcggtg
     1561 tgcagagtga agccgacatc ggtcatcgcg accgtgcgtt tcacctgcca cgacggcgac
     1621 acagtgttga cgcgggcggt gattgagacg tcgtctactg ccgtgaccgt ccatggcaag
     1681 cgcgcgatct ctccgtgcag cccgg
//
LOCUS       DASWVF010000812         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_48450, whole genome shotgun sequence.
ACCESSION   DASWVF010000812 DASWVF010000000
VERSION     DASWVF010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48450"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VGD55_14385"
     CDS             complement(<1..1483)
                     /locus_tag="VGD55_14385"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY0871582.1"
                     /translation="MKRIAAAVQEGASAYLKRQFRTLGVFAVIVFFLLLLLPADSGTQ
                     RIGRSIFFLVGAVFSGVTGYTGMWLAVRANLRVAAAARTGESRGAMRIAFRTGGVAGM
                     FTVGLGLFGACLVVLFFRANAPKVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVG
                     KVEQGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVASLILGKAAFGNLG
                     LVFPLIVPAIGVLTAVVGIFAVSPRAGDRSGMTAINRGFFISAAFSVVLVAVLAFTEL
                     PSKFSGLKGLTDSQIASHSGNPRVFALIAVVIGIALAAIIQQLTGYFTETNRRPVQDI
                     GKTSLTGPATVILAGVAVGLESAVYAALIVAAAVYGVFLLGGGITVLSLFAVALAGTG
                     LLTTVGVIVAMDTFGPVSDNAQGIAEMSGDVTGEGAVVLTQLDAVGNTTKAITKGIAI
                     ATAVLAATALFGSFTSAISDALAKAGTSAAGVTGITAASLSTDV"
BASE COUNT          304 a          490 c          516 g          213 t
ORIGIN      
        1 tgacgtctgt cgacaagctg gccgcggtga tgcccgtgac gccggcggcc gaggtacccg
       61 ctttcgccag cgcgtcgcta atggcagagg tgaacgaccc gaagagcgcg gtcgcggcga
      121 gaacggctgt cgcgatcgcg atgcccttgg tgatcgcctt cgtggtgttg ccaacagcgt
      181 cgagctgagt caggacgacg gcgccttcgc cggtcacgtc accagacatc tcggcgatgc
      241 cctgggcgtt gtcggacacc ggcccgaacg tgtccatcgc gacaatgaca ccgaccgtgg
      301 tcagcaggcc ggtgccggcg agcgcgacgg cgaacagcga cagcacggtg atgccgccgc
      361 cgagcaggaa gacgccatag acagccgcgg caacgatcag cgcggcgtag acggccgact
      421 cgaggccgac cgcaacaccc gcgagaatca ccgtcgcagg accggttagc gacgtcttcc
      481 caatgtcttg aaccggacgt cgattggttt cggtgaagta gcccgtcagc tgctggatga
      541 tggcggccag cgcgatcccg atgacgactg cgatcagcgc gaacactctc gggttgcccg
      601 aatgcgacgc gatctgggag tcggtcaggc ccttcagccc cgagaatttc gacggcaact
      661 cggtgaaggc cagcaccgct acgagcacaa ccgaaaacgc ggccgagatg aagaagcctc
      721 ggttgatcgc ggtcatgccg ctgcggtcac cggcacgcgg cgacaccgcg aagatgccga
      781 ccactgccgt gagcactccg atcgcgggca cgatcaaggg gaagacgagg ccgagattgc
      841 cgaaagccgc cttgcccagg atcagcgacg ccacgagggt gactgcgtag ctctcgaaaa
      901 gatcagccgc catgccggcg cagtcaccga cgttgtcacc gacgttgtca gcaatggtcg
      961 cggcgttgcg cgggtcgtct tctgggatcc cctgctcgac cttgccgacg aggtcggcgc
     1021 ccacatcggc ggctttggtg aagatgccgc caccgacacg catgaacatg gcgagcaagg
     1081 ccgcgccgaa cccgaatcct tcgagcacct tgggtgcgtt tgcacggaag aacagcacga
     1141 cgaggcaggc gccgaacaga ccgaggccga cggtgaacat gccggcgacg ccaccagtgc
     1201 gaaaggcgat ccgcatcgca ccgcgcgatt cgccagtgcg ggcggccgcg gcaacccgca
     1261 ggtttgcccg gacggcgagc cacatgccgg tgtatccggt gacgccggag aagacagcgc
     1321 cgaccaggaa gaagatcgag cggccgatgc gctgggtgcc cgagtcggct ggcagcagga
     1381 gcaagaggaa gaacacgatg accgcgaaca cgccgagcgt gcggaactgg cgcttcaggt
     1441 aggcgctggc accctcttgc accgcggcag cgatgcgctt catgttctcg gtgccttggt
     1501 cggcggaaag cacctcgcgc acg
//
LOCUS       DASWVF010000813         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_328330, whole genome shotgun sequence.
ACCESSION   DASWVF010000813 DASWVF010000000
VERSION     DASWVF010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328330"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="VGD55_14390"
     CDS             complement(<1..1754)
                     /locus_tag="VGD55_14390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY0871583.1"
                     /translation="MTAPSWTAKAAKTGAAASTAQVAARVYLAPQGGLDSLASLATSI
                     ATPGSASYRQILTAAQYQAQFAPTAASVQAVTAFLSSNGLTVTGTGAENSYVAFTGTV
                     AQADKAFGVTINVYKRNGAKVQAPAGPVNLPDSLASSVLTVEGLDTSEHYATPPRPAA
                     APPPGFRNARPCSTYYGQIPATYQADFSTPLPQFDGKTLAYAPCGYTGPFLRSAYEGN
                     TSLDGSGVTVAITDAYAAPTIAKDAQTYATRNGDGSYAPGQLTQTDASSFTHAAKCGP
                     SGWYGEETLDVEAVHAMAPGANIHFYGAASCFDNDLLDSLSQAVIDDSAQLVTNSWGE
                     PEESEDASLIPAYEAVFLQGAVEGISFMFSSGDDGDELANTGTRQSDYPTSDPYVTSV
                     GGTSTEIGSTGQIIDATGWGTVKYSLSSTGTAWNLVGFLYGSGGGFSSLFNKPAYQGS
                     AVPGAYRGVPDVAMDADPNTGMLIGETQTFPDGKYYDQYRIGGTSLASPLFAGLTALA
                     IENAGSPVGLLNPTIYGNAAAFTDVKGKPKSEGDVRVDFANGIDGTGGLLYSVRAFNQ
                     DSSLSINKGWDDVTGLGSG"
BASE COUNT          274 a          598 c          636 g          316 t
ORIGIN      
        1 cccgagccga gcccggtcac gtcgtcccaa cccttattga tgctcagcga gctgtcctga
       61 ttgaaggcgc gcaccgagta cagcaacccg cccgtgccat cgatgccgtt ggcgaagtca
      121 acgcgtacgt caccctccga cttgggcttg cccttcacgt ctgtgaacgc ggcggcgttg
      181 ccgtagatcg tcgggttaag caacccgacc ggcgagccgg cgttctcgat cgccaaggca
      241 gtgagcccgg cgaacagcgg agacgcgagg ctagtgccac cgatgcggta ctggtcgtag
      301 tacttgccgt ccgggaaggt ctgagtctcc ccgatcagca tgccggtgtt cgggtcggcg
      361 tccatcgcca cgtcgggcac cccgcggtac gcgccgggca ccgccgaccc ctggtaggcc
      421 ggcttgttga acagcgacga aaagccgcca cctgacccgt acaggaaccc gaccaggttc
      481 cacgccgtgc cggtgctgga cagcgagtac ttcaccgtgc cccagccggt cgcgtcgatg
      541 atctggccag tgctgccgat ctcggtggac gtgccgccga cgctggtcac gtacgggtcg
      601 gacgtggggt agtcggactg ccgggtgccg gtgttggcca gctcgtcgcc gtcgtcacca
      661 gaggagaaca tgaagctgat cccctcgacc gcgccctgta ggaacacggc ctcgtaggcg
      721 gggatgaggc tcgcgtcctc ggactcctcg ggctcgcccc aagagttcgt caccagctgt
      781 gcggagtcgt cgatgaccgc ctgcgagagc gagtccaaca agtcgttgtc gaagcaactc
      841 gccgcgccat agaagtggat gttcgcgccc ggtgccatgg catggacagc ctcaacgtcg
      901 agggtctcct cgccgtacca ccccgaaggg ccgcacttgg ccgcgtgcgt gaacgaactc
      961 gcatcggttt gggtgagttg gccgggggcg tagctgccgt ccccgttcct cgtcgcgtag
     1021 gtctgtgcgt ccttcgcgat cgtcggcgcc gcgtaggcgt cagtgattgc gacagtcacg
     1081 ccagagccgt cgagcgaggt gttgccctcg tatgccgacc gcaggaacgg accggtgtag
     1141 ccgcacggcg cgtaggccag cgtcttgccg tcgaactgcg gcagcggtgt cgagaagtcc
     1201 gcctggtatg tcgccgggat ctggccgtag tacgtcgagc acggcctcgc gttgcggaag
     1261 cccggcggtg gagctgcagc aggcctcggc ggcgtggcgt agtgctctga cgtgtcgagt
     1321 ccctcgaccg tgagcacgct ggacgcgagc gagtcgggca ggttcacggg tccggcgggt
     1381 gcctgcacct tggcgccatt gcgcttgtag acgttgatgg tgacgccgaa cgccttgtcg
     1441 gcctgggcga cggttccggt aaaggcgacg tagctgttct ccgcgcctgt gccggtgacc
     1501 gtgagcccgt tgctgctcag gaaggcggtg acggcctgga ccgaggcagc cgtcggcgcg
     1561 aactgcgcct gatactgcgc cgccgtcagg atctgccggt agctcgcgct gccaggcgtc
     1621 gcgatcgacg tggccagcga cgccagcgaa tcgagtccgc cctgtggcgc gagatagacg
     1681 cgggccgcga cctgcgcggt cgaggccgct gcgcccgtct ttgcggcctt cgcggtccag
     1741 ctaggcgcag tcacggccag cggcttcgac gagttcgccg cggatgctgg tacggcggcg
     1801 gcgaggccgc cgataactcc gatc
//
LOCUS       DASWVF010000814         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_482156, whole genome shotgun sequence.
ACCESSION   DASWVF010000814 DASWVF010000000
VERSION     DASWVF010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482156"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGD55_14395"
     CDS             <1..638
                     /locus_tag="VGD55_14395"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEY0871584.1"
                     /translation="IATDPELQRRVRVDMYRRLGFYPTETSEHSAEYVPWYLHDDAEI
                     TRLRIPVRDYVGISARNAAEIEQLIPRAAAGDYIEPEEDAAEYAPQVIHSLVTGQRRE
                     IQVNVANRGLISNLPDGAPVEVPCRIDGSGVSPVAVGALPPQCAALNHAFLSVAELTV
                     RAFTEGRPDHIRHALMVDPNTSATLSPGRIFELADAMVGAHRDRLPEVLRN"
     gene            complement(658..1425)
                     /locus_tag="VGD55_14400"
     CDS             complement(658..1425)
                     /locus_tag="VGD55_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY0871585.1"
                     /translation="MRQQDRLGLILERLSRQSSVSVPELAAQLEVSQASVRRDLQLLE
                     SQQLLSRTHGGAVASGVLYELPMRYRGGQRHDAKRAIAKLAVQLLGPEVSSVGLNGGS
                     TTTEVARALATRPGLRVVTNALNIASELAVRSNIDLVVCGGSARTESYELVGPLAEMT
                     LMNINIEVAIIGVDGVNVTAGLTTHHEVEAQTNRCLLRAAERVIVVADSTKIGRRGFA
                     RISEIGVVADLVTDSDVKDSDVAELERAGLRVHRAGV"
BASE COUNT          310 a          565 c          531 g          283 t
ORIGIN      
        1 ggatcgccac cgaccccgag ctgcaacggc gggtgcgggt cgacatgtac cgcaggctgg
       61 gcttctaccc gacagagacg agcgagcatt ccgcagaata cgtgccgtgg tatttgcacg
      121 acgacgcaga gatcacccga ctgcgcatac cggtacgcga ctacgtcggc atcagcgcgc
      181 gaaacgcggc ggagatcgaa caactcatcc cccgggccgc cgctggtgac tacatcgagc
      241 ccgaggagga cgcggccgaa tacgccccgc aggtgattca cagcttggtt accggccagc
      301 gtcgggaaat ccaggtcaac gtggcgaacc gaggtctgat cagcaacttg ccggacggcg
      361 cgccggttga ggtgccctgc cgaatcgacg gatccggcgt gagcccggtc gcggtcggtg
      421 cgctgccacc gcaatgtgcg gcgctgaacc acgcgttcct gtccgtcgct gagctgacgg
      481 tgcgtgcgtt caccgaaggc cggcccgatc acatccggca cgcgctcatg gtcgacccga
      541 acacgtccgc gacgctgtcg ccgggtcgca tcttcgaact cgctgacgcc atggtcggcg
      601 cgcaccgcga ccgtctcccc gaggtgctgc ggaactgaag ctgcagactc aggcggatta
      661 gacgccggcc cgatgaacgc gtagtcctgc gcgctcgagt tcggcaacgt cgctgtcctt
      721 cacatctgag tcggtgacca ggtcagcgac gacaccgatc tcactgatgc gcgcgaatcc
      781 gcgacgaccg attttcgtcg agtcggccac cacaatcacc cgctcggcgg cgcgcagcag
      841 gcaccggttg gtctgcgctt cgacttcgtg gtgcgtggtc aggccagcgg tgacgttcac
      901 gccgtcgacg ccgatgatcg cgacctcgat gttgatattc atcaacgtca tctcggcaag
      961 cgggcccacc agctcatagg actccgtgcg cgcgctcccg ccgcagacga cgagatcaat
     1021 attcgaccgg acggcgagtt cggacgcgat gttcagcgca tttgtcacga cccgaaggcc
     1081 gggtcgggtg gccaaagccc tggcaacctc agtcgtcgtc gaaccaccgt tgaggccgac
     1141 cgagctaact tcagggccga gcaactgcac ggcgagcttg gcgatcgccc gcttcgcgtc
     1201 gtgccgctga ccacctcgat accgcatcgg cagttcgtac agcacgcccg acgcgacggc
     1261 cccgccgtga gtgcggctga gaagctgctg gctttccagc agctggaggt ctcgtcgcac
     1321 ggacgcctga gacacctcga gctgcgccgc gagttcgggc acactcaccg acgactgcct
     1381 gctcaaacgc tcaagaatga gtccgaggcg atcctgttga cgcatagcgt gcacgttacg
     1441 cgcaaaggac ccgcgatgca gcaggaggcg gccccggctt tcgtcgggtt tcaagcgtgt
     1501 ttccggggga tctcggcctc tcgaggacct tgaccatgcc tgcgcaaacg tgcaactctg
     1561 cacatctaca gcgatcgaat atgcgcagat caaggagacc ctgatggaat cggtcggcac
     1621 tcgcacccgg gttatcgcca gtgcaaccgt gctgctcgtg gcgctcgccg cgtgcagctc
     1681 gaagtcgtc
//
LOCUS       DASWVF010000815         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_252524, whole genome shotgun sequence.
ACCESSION   DASWVF010000815 DASWVF010000000
VERSION     DASWVF010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252524"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGD55_14405"
     CDS             complement(<1..215)
                     /locus_tag="VGD55_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871586.1"
                     /translation="MNRVGAVLAAATPRPVSVKVLPFGSRADLIVGIIAVFAGLLGLL
                     LLLAFNSSLLDTRDTRVRRRLSFYTLTG"
     gene            complement(212..1654)
                     /locus_tag="VGD55_14410"
     CDS             complement(212..1654)
                     /locus_tag="VGD55_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0871587.1"
                     /translation="MSLADRLAQARRSRDAGANSTTQALDAHPSTARRVADPFLELKR
                     TVHTTLLDNLGPQLYDTRFTQADLEQKVRHTLQGVLAQDETPLSVADRTRIAQEIADD
                     ILGYGPLEPFLRDSEVSEIMVNGPNQIYVERSGRLHAVAASFVDEAHLRRTIDKIVAQ
                     VGRRVDETSPMVDARLADGSRINAVVPPIAVDGSLLTIRKFATDPYEVDDLIGFGTMT
                     SDLAEFLRGCVKGRLNILVSGGTGAGKTTTLNVLSSFIPADERIITVEDAAELQLHQE
                     HVLRLEARPPNIEGRGEIRIRDLVRNALRMRPDRIVIGEVRDAAALDMLQAMNTGHDG
                     SISTVHANSPKDVLARIETMALMAGMELPVRAIREQMASAFDVIVHQARFKDGSRLIT
                     CVSEVVGMGDGGDTVELNDLYVFDHSPGYDDSGRPLGELGPTALLPAFLDKLGAAGIN
                     LNGSVLDRANHTHHENENHGDSHPHEDYSS"
     gene            complement(1651..2853)
                     /locus_tag="VGD55_14415"
     CDS             complement(1651..2853)
                     /locus_tag="VGD55_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0871588.1"
                     /translation="MSVIVEPDPTRAETLRSALGPGVLVLKTLDELRRHLEAHPGEDA
                     VVLGPSVDQDAAFRLSDGMRITRTSLGVVLVRRRIDTPLLVDALRNGVREVVEERNLA
                     GVNDAVRRERDVAAAMRQRNSHGEGEDGRRGVVVTVFSAKGGCGKTTLASNLAASLAD
                     GGHRDVCLVDLDLNFGDVAIVLQLFPAHTLADAVPLLETLDLPAVLALLTPHSPGLSA
                     LVAPVEPGSAETVPASLITTILQLLREEFAFVIVDTPPAFTDHVLAAFDQSDVIALLA
                     TLDIPALKNLKLTLETLDLLNYPRERLRVVLNRADSKVGLALSEVEKTLRVPIALHIP
                     SSRAVPASINRGVPIMLDDPSHPVSLAIRQFGDDHVLPLARAGFDPIASNMRNDRRGP
                     FRRRSKTV"
     gene            complement(2864..3568)
                     /gene="cpaB"
                     /locus_tag="VGD55_14420"
     CDS             complement(2864..3568)
                     /gene="cpaB"
                     /locus_tag="VGD55_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0871589.1"
                     /translation="MGRRTVLLVAAILVAALGAGLVLIYVHDVDQREKAREAPEQILV
                     AKKIIPAGTSGATASAEGDLEFMKVARAAVAPGALASIDPVSNLVALAPIYPGEQILS
                     AKFGKQGAQSLLTIPTAGTMALSVSLSGPARVNGFLAPGSQVAVFLTAGGSTKLILSR
                     VTVIAVGTRTLVPSSGQATGADQQSDVVTFGVTAAQAQILIYAQSTGSLYLSLLTADS
                     TVPSLPPTNSTNITQG"
     gene            complement(3749..>4010)
                     /locus_tag="VGD55_14425"
     CDS             complement(3749..>4010)
                     /locus_tag="VGD55_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871590.1"
                     /translation="SRAELRGHRDTPAPPASMAIYSVRLATITTLVGLLFSLAAHQHL
                     WFGALVLASGFVAISGVSIARSFRRWDDPVVRSRVVAAVSNG"
BASE COUNT          716 a         1222 c         1408 g          664 t
ORIGIN      
        1 cccgtgagcg tgtagaacga aaggcgcctg cgcacgcgcg tatcgcgggt atcgagcaac
       61 gaggagttga aagccagcag cagcaggagt ccgaggagtc cggcgaacac cgcgatgatg
      121 ccgacgatga gatccgcgcg cgagccgaag gggagcacct tcacgctgac gggccgcggc
      181 gtcgctgccg ccagtacggc gccgacgcgg ttcatgagga atagtcctcg tgcgggtgcg
      241 agtcgccgtg gttctcgttc tcgtggtgcg tgtgattggc ccggtcgagt accgacccgt
      301 tgaggttgat gccggccgca ccaagcttgt cgaggaaggc cggcaaaagt gcggtcgggc
      361 cgagctctcc aagcggccgg ccggagtcgt cgtacccggg ggagtggtcg aagacgtaga
      421 ggtcattgag ctcgaccgtg tcgccgccgt cacccatgcc gacgacttcg ctcacgcagg
      481 tgatcagccg gctgccgtcc ttgaagcgtg cttgatggac gatcacgtcg aatgcgctcg
      541 ccatctgttc acggatcgcg cgcacgggca actccatacc ggccatcaac gccatcgttt
      601 cgatgcgggc cagcacgtct ttcggtgagt tcgcgtggac cgtgctgatc gagccgtcgt
      661 ggccggtgtt catggcctgc aacatgtcga gtgccgctgc gtcgcgaacc tcaccgatga
      721 cgatgcgatc gggccgcatg cgcagcgcgt tgcgcaccag atcacggatg cgaatttcgc
      781 cgcggccctc gatgttgggt gggcgggctt cgaggcgcag cacgtgttct tggtgcaact
      841 gaagttcagc cgcatcttcg accgtgatga tgcgctcgtc ggctggaatg aacgacgaga
      901 gcacgttcaa cgttgtcgtc ttccccgcgc cggtgccgcc ggacaccaag atgttaaggc
      961 ggccttttac gcacccgcgt aagaactcgg cgaggtcgga ggtcatggtg ccgaagccga
     1021 tgaggtcgtc gacttcgtag gggtcggttg cgaacttgcg aatcgtcagc aacgagccgt
     1081 cgacggcgat cgggggcacg acggcgttga tacggctgcc gtcggcaagc cgtgcgtcga
     1141 ccatggggct ggtctcatca acccgacgtc cgacttgcgc gacgatcttg tcgatcgtgc
     1201 gccgcaggtg tgcttcgtcg acgaaggagg cagcaacagc gtgcagccgg ccactgcgct
     1261 cgacgtagat ctggttcggg ccgttgacca tgatctcgct gacctcggag tcacgcagga
     1321 agggctcgag ggggccgtaa ccgaggatgt cgtcggcgat ctcctgcgcg atgcgggtgc
     1381 ggtcggcaac cgagagcggg gtttcgtctt gcgcgagcac gccttgcagc gtgtgacgca
     1441 ccttctgctc gagatcggcc tgggtgaacc tcgtgtcgta cagctgcggg ccgaggttgt
     1501 cgagcagcgt cgtgtgcacg gtgcgcttca gttcaaggaa gggatcggcg acgcgacgcg
     1561 cggtcgacgg gtgcgcgtcg agtgcctgcg tcgtggagtt agcgccggcg tcacgcgacc
     1621 ggcgagcttg ggcaaggcgg tcggcgagac tcatacggtc ttcgacctcc tccggaacgg
     1681 cccacgccga tcatttcgca tattcgaggc aatcggatcg aacccggcgc gcgcaagggg
     1741 aagcacgtga tcgtcgccga actgcctgat cgccaggctc accggatggc tggggtcgtc
     1801 gagcatgatc ggcacaccgc gattgatgga cgccgggaca gctcgcgacg agggaatgtg
     1861 cagcgcgatc ggcacccgca acgtcttctc gacctcggac aacgcgagcc ccaccttgga
     1921 gtcggcgcgg ttcaagacaa cgcgcagccg ctcacgcgga tagttgagca gatcgagggt
     1981 ctcgagcgtc agcttcaggt tcttcaaggc cgggatgtcg agcgtcgcga gtaaggcgat
     2041 cacgtcggac tggtcgaatg cggcgagcac gtggtcggtg aacgccggcg gggtgtcgac
     2101 gatgacgaac gcgaactcct cgcgcagcag ctgaaggatc gtcgtgatga gcgatgcagg
     2161 caccgtctcg gcagacccag gctcgacggg agcgaccagt gccgacagcc cgggcgagtg
     2221 cggtgtcagc aatgccaaga cagcgggcaa gtcgagtgtt tcgagcagcg gcactgcatc
     2281 ggcgagtgtg tgcgcaggga acagttgcag gacgatggcg acgtcaccga agttgaggtc
     2341 gaggtcgaca agacacacgt cgcgatggcc gccgtcggcg agtgacgcgg cgagattgct
     2401 tgccagcgtg gtctttccgc acccgccttt cgccgagaac acggtgacga ccactccgcg
     2461 ccgaccgtct tcgccctcac catgcgaatt gcgctgacgc atcgccgcag caacatcgcg
     2521 ctcccgccgt accgcgtcgt tgaccccagc gaggttgcgt tcttcgacga cctcgcggac
     2581 gccgttgcga agagcgtcga cgagcaacgg ggtatcgatg cgcctgcgga ccaggacgac
     2641 tccgaggctg gtgcgggtga tgcgcatgcc gtcgctcagc ctgaaagcgg cgtcctggtc
     2701 aaccgatggc cccagcacga cggcgtcctc gcctggatgc gcttcgaggt gacgacgcaa
     2761 ctcgtcaagc gtcttgagga caagcacgcc gggaccaagt gcagaccgca gggtctctgc
     2821 tcgggtcgga tccggttcga cgatgacact catgaggggg cgctcacccc tgagtgatgt
     2881 ttgtcgagtt ggtcggtggc agcgacggga cggtcgagtc ggccgtgagc aggctcaggt
     2941 aaaggctgcc ggtcgactgc gcgtagatca ggatttgagc ctgcgcggcg gtgacgccga
     3001 acgtgacgac gtcggattgc tggtcggcgc cggtcgcttg tcctgacgac ggcacaagcg
     3061 tgcgggtacc gaccgcgatg accgtgacgc gggagaggat caacttcgtc gagccaccgg
     3121 cggtcaggaa gacggcgacc tgtgagccag gtgcgaggaa gccgttgacc cgggcggggc
     3181 ccgacagcga cacgctcaac gccatcgtgc ccgcggtcgg gattgtcagc aggctctgcg
     3241 ctccctgctt gccgaacttc gcgctgagga tctgctcacc ggggtagatc ggcgccagcg
     3301 cgaccaggtt cgagaccgga tcgatcgagg ccagggcgcc gggagcgacg gctgcgcgag
     3361 cgaccttcat gaattcgagg tcaccttcgg cggacgccgt cgcgccgctc gtgcccgcgg
     3421 gaatgatctt cttggcgacg aggatctgtt cgggtgcctc gcgagccttc tcgcgctggt
     3481 cgacgtcgtg cacgtaaatg agcaccaggc cggcgccgag tgctgcgacc aggatggcgg
     3541 cgacgagcag gaccgtgcgg cgtcccattc ggcgtcccac tcctctcggg tgcacaagag
     3601 cgcccggaag tggccgcact tgtcgcgaat actcccacct tcgtgggccg ccgggcgggg
     3661 ctaggcgcga aggacacgaa cactcttgca gctaagccta cggaaggggc gagccagaac
     3721 gcgtgcagcg gtccagcagt ggcaactgtc agccgttcga gacggcggcc acaacccttg
     3781 aacgaacgac cggatcgtcc caacgtctga agctgcgggc gatcgagaca ccgctgatcg
     3841 caacgaaacc cgacgccaac accagagcgc cgaaccatag gtgctggtga gcggccaacg
     3901 agaagagcag gccgacgagc gtggtgatcg tggccagccg caccgagtag atcgccatcg
     3961 aagccggggg cgcaggggta tcgcgatggc cacgcagttc ggcgcgactc
//
LOCUS       DASWVF010000816         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_456881, whole genome shotgun sequence.
ACCESSION   DASWVF010000816 DASWVF010000000
VERSION     DASWVF010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456881"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1935)
                     /locus_tag="VGD55_14430"
     CDS             complement(196..1935)
                     /locus_tag="VGD55_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0871591.1"
                     /translation="MATEVAVEAPASAGPQLLPASNARLFGGFVGRTWLWFLGGCLAV
                     TLLPLLFGWRPYVVESGSMAPRIKVGDVILAAPEHNPQKLLGRVTVFHDPDPGRIGSV
                     KSHRVVAINSDGTLTTKGDANQSVDSVHVRVSSVIGLGRLLVRYVGLPLIWAQKGQWM
                     QLGLLAASIWFAALLVMRDRDGMVIEPVDPDAPSDPPDPPDPGAPAAPDPETSSARVA
                     AEAAGRSIPQPRSEGGRFLPKPRPHPASPQGQSEASARVPLVRHWHVRTSRVWRSPFT
                     RFAGKLARRTAYAGVLSLALVLPTARAAFSAKTLNTADSWVVPTYNYTTETNNLGPYL
                     YWKLDDATASTTAADTSGNGRTGTYNSTWTKNQAGALVDQTPNLGVTETNAAAPAANT
                     SCIYTTSATGMATPGPMTYSEVIWFKTTSTTGGKLIGLENNRTGVSDSSGPGGQYDRM
                     IYMDANGELWFGVYNATEIVAHSQAGLNDGAWHMAAATMSTTTGMSLYVDGKLVGTNA
                     NTVSETETKTSFWRVGCGNLAGWGGDWTGANPPGNAAANNLNYPFNGSLDEATVWFST
                     LTATQIAFLYWIH"
     gene            complement(2068..>2770)
                     /locus_tag="VGD55_14435"
     CDS             complement(2068..>2770)
                     /locus_tag="VGD55_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871592.1"
                     /translation="LVIKSTPSATEQLAKSIHYLGAKHMSEQPKQSERRRRVVRATAP
                     AFGLLAAGLLVWQGSYAAFSATTVDTNNAWASGSLALTNNGGGAVYSAATSATFGGAN
                     LKPGSTGTTCLTVKSVGTSAGTLAMYESSLVDSAPSLGAQIQLTITAGVPATDVQANC
                     TGFPVAGLTTVATNQPLSTFAGTYATGVPIAVATGTVLEAYKVTWTFASTGTNPGDTA
                     LMGKTVTAGFTWEIQ"
BASE COUNT          462 a          844 c          970 g          494 t
ORIGIN      
        1 ttcgacgttg ccgatcaggc gcgccgcgac ggccgaggca agcgcacgcg aggtccccgg
       61 gtcgtcgggg agcgcggcca acgcggggcc ggtggtcata ggtcctccgg atcgggatgg
      121 acacgttcaa tgactcatcg gccaccgagc gccttctatg aaggttgatg aaggtcgatg
      181 agggctgttc agggtttagt ggatccagta aaggaaagcg atctgcgtcg ccgtgagcgt
      241 gctgaaccag acggtcgcct cgtcgagcga gccgttgaac gggtagttga ggttgttcgc
      301 agcggcattt ccgggcggat tagcgccagt ccagtcgcct ccccagccag ccaggttgcc
      361 gcagccgacc cgccagaaac ttgtcttggt ctccgtctcc gagaccgtgt tggcgttggt
      421 gccgacaagc ttgccgtcga cgtagagaga catgccggtc gtcgtgctca tcgtggcggc
      481 cgccatgtgc cacgcgccgt cgttcaggcc ggcttgcgaa tgcgccacga tctcggtggc
      541 gttatagacg ccgaaccaga gctcgccgtt ggcgtccatg tagatcatgc ggtcgtactg
      601 gccgccgggt ccggaactgt ctgagacgcc ggtgcggttg ttctccagac cgatgagctt
      661 gcctccggtc gtcgacgtgg tcttgaacca gatcacttcg ctgtaggtca tcgggcctgg
      721 tgtcgccata ccggtagcgc tggtcgtgta aatacaagac gtgttcgcgg cgggcgcagc
      781 ggcgttggtc tccgtcacgc caaggttggg tgtctggtcg accaacgcgc ccgcttggtt
      841 cttcgtccag gttgagttgt acgtacccgt gcgaccgttg ccggatgtgt ccgccgccgt
      901 agtgctggcc gtcgcgtcgt cgagcttcca gtagaggtac gggccgaggt tgttcgtctc
      961 ggtcgtgtaa ttgtaggtcg ggacgaccca tgagtcggcg gtgttgaggg tcttcgcgga
     1021 gaacgccgcg cgggcagtcg gcaacacgag cgcaagagaa agcacgcctg cgtatgccgt
     1081 tcgtcgcgca agcttgcctg caaagcgcgt gaagggcgag cgccaaacgc ggctggtccg
     1141 aacatgccaa tgccgcacga gtggtactcg cgcggaagct tctgactgac cctgcggact
     1201 cgccggatga ggccggggct tgggtaagaa ccggccgcct tcggatcgcg gttgcggaat
     1261 cgaccgaccg gccgcttcgg cggcgacccg cgcactcgac gtctccgggt ccggtgccgc
     1321 gggtgcacct ggatcaggcg ggtcaggtgg gtcggaggga gcgtcgggat cgaccggttc
     1381 gatcaccatg ccgtcccgat cacgcatcac gaggagtgcg gcgaaccaga tcgacgctgc
     1441 gagcaggccg agttgcatcc actggccctt ctgggcccag atcagcggca accccacgta
     1501 cctcacgagc agccggccaa gaccgatcac cgacgagacc cggacgtgaa cgctgtcgac
     1561 cgactggttc gcgtcaccct tcgtcgtgag cgtgccgtcg gagttgatcg cgacgacccg
     1621 gtgcgacttg acgctcccga tacgaccggg gtcagggtcg tggaagaccg tcactcggcc
     1681 gagcaacttc tgcgggttgt gctccggcgc agcaaggatg acatcgccga ccttgatccg
     1741 cggagccatc gaccccgact cgacgacgta gggccgccaa ccgaacagca agggcaacaa
     1801 cgtgacagca agacagccgc cgaggaacca caaccaggtg cggccgacga agccgccgaa
     1861 cagcctggcg ttactcgcgg gaagcagttg cgggcctgca gatgcaggcg cctccaccgc
     1921 gacctcggta gccaccagac ttcctttcat tgggcgtttg tctctaacgc tgtggtgtct
     1981 ccatcaaaag ccgccggctc ccgtgccccc ggttgtgcgg gggcccggga gccggcggtt
     2041 agttcgacga gatgtcgagc aaggctctta ctggatttcc catgtgaagc cagcggtgac
     2101 cgtcttgccc atgagggcgg tgtcacctgg gttggtgcca gtgctggcga acgtccaggt
     2161 gaccttgtag gcctcgagga cagtgccggt ggcgacagcg atcgggacgc cggtcgcgta
     2221 ggtgcccgcg aacgtcgaca gaggctggtt cgtggcgacc gtcgtgaggc ccgccaccgg
     2281 gaagccggtg cagttcgcct ggacgtcggt cgcaggcaca cccgcagtga tggtcagctg
     2341 aatctgcgcg cccaacgacg gtgccgagtc gaccagcgaa ctctcataca tcgccaaggt
     2401 gccggcggac gtgccgacgc tcttgaccgt gagacaggtc gtgccggtcg aaccaggctt
     2461 gaggttggcg ccgccgaacg tcgcgctggt cgcggcggag tagaccgcgc cgccgccgtt
     2521 gttcgtcaga gccagtgagc cggacgccca cgcgttgttg gtgtcgacgg tcgtggcgga
     2581 gaaggcggcg tagctgccct gccacacgag gaggccggcg gccaggaggc cgaaagccgg
     2641 ggcggtggca cggacgacgc ggcgccggcg ctcggactgc ttgggctgct cggacatgtg
     2701 ttttgctccc aggtagtgga tggacttggc gagttgttcg gtcgcagaag gcgtggactt
     2761 gatgactaag
//
LOCUS       DASWVF010000817         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_482231, whole genome shotgun sequence.
ACCESSION   DASWVF010000817 DASWVF010000000
VERSION     DASWVF010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482231"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..812)
                     /locus_tag="VGD55_14440"
     CDS             complement(102..812)
                     /locus_tag="VGD55_14440"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0871593.1"
                     /translation="MPAPRERTHELVLRRIEADLSEGRVSLGDRLPGERGMAENLGVS
                     RASVREAIRVLEAMGIVRTAVGSGSEAGAIIVADPSAALTAALRLHLATSHLLIADIV
                     ATRVLLESWAVRVAAASRDRGQFEKAQDLLNEMDDEGLKPEQFHRLDAEFHIELTRQA
                     GNALVAAVMASLRESIQGYVLAAVPRLPDWPAMARKLRREHRRVLAAVLAHDGTAAAV
                     AVTKHIEGFYRATGLGRA"
     gene            892..1650
                     /locus_tag="VGD55_14445"
     CDS             892..1650
                     /locus_tag="VGD55_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003794770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0871594.1"
                     /translation="MRIALFATCLGDALYPDVSKATVRVLERLGHTVEFPAAQTCCGQ
                     MHINSGYLDEAAPLVRHHVETFEAALAHCDAIVAPSGSCVGSVRHQHAMVARTRGLEG
                     GTALAERAEAVAAKTFELSEFLVDVLGVTDVGAYYPHRVTYHPTCHSLRLLHVGDKPA
                     QLLRAVRGLELIELPAAESCCGFGGTFAIKNSDVSTAMLADKMSNVLSTKAEVCTAGD
                     SSCLMHIGGGLSRLRAGVRAVHLAEILASTEDAP"
     gene            1647..>2061
                     /locus_tag="VGD55_14450"
     CDS             1647..>2061
                     /locus_tag="VGD55_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871595.1"
                     /translation="MTFLGMPSYAPDGVGNLRGDKTFPHAARSALKNAQLRRNIGVAT
                     STIRSKRAAVVGEVPDWEQLRDAGSAIKTDVMARLPFLLEQLEAAVIARGGVVHWAPD
                     ATEANRIVVDLVQATGAAEVVKVKSMATQEIGLNEA"
BASE COUNT          342 a          687 c          685 g          347 t
ORIGIN      
        1 gtcttcgtca accgcctcga acaggccggc aatcggggcg ccgccgaatc cgtagcgacc
       61 cacgcgcagg ccgccgcgac cgagggggag tagctgcgac atcacgcccg accaagaccg
      121 gtggcccggt agaagccttc gatgtgcttg gtgacggcaa ccgcagccgc tgtgccgtcg
      181 tgcgcgagca ccgccgcgag cacccgccgg tgctcgcgac gcagcttgcg cgccatcgcc
      241 ggccaatcgg gcagccgtgg gacggccgcc aacacgtagc cctggatcga ttcacgaagc
      301 gaggccatca cggccgcgac cagcgcgttg ccggcctgcc gggtgagctc gatatggaat
      361 tcggcgtcga gccggtggaa ctgctcgggc ttcaacccct cgtcgtccat ctcgttcaag
      421 aggtcttggg ctttctcgaa ctggccccga tcgcgcgacg ccgcggcgac gcgcacggcc
      481 cacgactcga gcagcacgcg ggtagcgacg atgtcggcaa tgagtaaatg acttgttgcc
      541 aggtgcagcc gcaacgccgc cgtcaacgca gcagacgggt cggccacgat gatggcgccc
      601 gcctcgctgc ccgatccgac cgcggtgcga acgatcccca tcgcttcgag tacccgaatc
      661 gcctcgcgca ccgaagcgcg gctcacgccg agattctcgg ccatgccgcg ctcgccgggc
      721 agccggtcgc cgagggagac ccgcccctcg gacaggtcgg cttcgatgcg gcgcagcacg
      781 agttcgtggg tgcgctcgcg cggggcgggc atgactatga ccggtccttc ccgtcgctgc
      841 tgggctagtg tggtctgacc acaccgcgcg actgtacccc cgggagtcaa gatgcgcatc
      901 gcgttgttcg cgacgtgtct tggcgacgcg ctgtatcccg acgtcagcaa ggccacggtt
      961 cgggtactcg aacgcctcgg gcacacggtg gagtttcccg ccgcgcagac ctgctgcggt
     1021 cagatgcata tcaacagtgg ctacctcgac gaggcggctc cgcttgtgcg tcatcacgtc
     1081 gagaccttcg aagcagcact ggcccattgc gatgccatcg tggcgccgtc cggctcatgt
     1141 gtcggctcgg tgcgccatca acacgcgatg gtggcgcgca cccgaggtct cgagggcggg
     1201 acggcgcttg ctgagcgtgc cgaggcagtt gccgcgaaga cgttcgagtt gtctgagttc
     1261 ctcgtcgacg tgctcggcgt caccgatgtc ggtgcctatt acccccaccg cgtgacgtac
     1321 cacccgacct gccattcgct gcgccttctt catgtcggcg ataagccagc gcaattgctt
     1381 cgagcagtac gcggccttga gttgatcgag ttacctgctg ccgagtcgtg ctgtgggttc
     1441 ggcggcacat tcgccataaa gaattcggac gtgtcgaccg cgatgctcgc cgacaagatg
     1501 agcaatgtgc tgtcgacgaa ggcggaggtg tgcaccgccg gcgactcgtc gtgcctcatg
     1561 cacatcggcg gcgggctttc gcgactacgg gcaggcgtcc gcgccgtcca tcttgccgag
     1621 atcctggcga gcaccgaaga cgcgccatga cctttctcgg tatgccgtcc tacgcacccg
     1681 atggcgtcgg aaatctgcgc ggcgacaaga catttccaca cgctgcgcgc tcagccctga
     1741 agaacgcgca actgcgacgc aatatcgggg ttgccacctc gacgatccgt tccaagcggg
     1801 cggcggttgt cggcgaggtg cccgactggg agcagctacg cgacgcaggc tccgcgatca
     1861 agaccgatgt gatggcacgg ttgcctttct tgttggaaca gctcgaggca gccgtcatcg
     1921 cacgcggcgg cgtcgtgcat tgggcgcccg acgcgaccga agcgaaccgg atcgtcgtcg
     1981 acctggtgca ggcgacgggt gcggccgagg tcgtgaaggt caagtcgatg gcgacgcagg
     2041 agatcgggct gaacgaggcc c
//
LOCUS       DASWVF010000818         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_150553, whole genome shotgun sequence.
ACCESSION   DASWVF010000818 DASWVF010000000
VERSION     DASWVF010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150553"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..459
                     /locus_tag="VGD55_14455"
     CDS             127..459
                     /locus_tag="VGD55_14455"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HEY0871596.1"
                     /translation="MNDQHIAVVLPYEPRRDDLLPDPEALRELALAENELVGILGASA
                     EFVAAVTAEGRRTLHFYADSENPHPAAVIRQWVHDNPEARWTIAVDPGWSAVAALARA
                     PLQPQGGE"
     gene            complement(479..1633)
                     /locus_tag="VGD55_14460"
     CDS             complement(479..1633)
                     /locus_tag="VGD55_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEY0871597.1"
                     /translation="MTLVDPRAVPIPRYGTGSLADLVPSVLASIGTPTFTNVLSLAPA
                     AAACILLIDGLGAELVRAHSADAPLLHAALVGGSGRDLTSGFPSTTAVSIASIGTGLP
                     PGAHGILGYQVAIPGTARLMNSLRWDSEVDPVEWQPLPTAFERAVTAGTSVVEVAKRE
                     FEGGGLDRAVLRAASFVGSDTFGEVGAGALAQLKEAVTARRPCLVYAYVADLDWTGHG
                     HGVGSLAWRLQLQLVERLVEQIVERLPAGSVLYVTADHGMVDIEASRRVDVEAEPWLL
                     DGVRLMGGEPRARYLYVRPGGLDDVMSGWTSRLGSDAVVCTRDEAIEAGWFGVVSDPY
                     RDRIGDVVVAALADIAVVDSRRHPGEAGLIGHHGSLTAAEQLVPLACFTG"
     gene            complement(1637..2248)
                     /locus_tag="VGD55_14465"
     CDS             complement(1637..2248)
                     /locus_tag="VGD55_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEY0871598.1"
                     /translation="MAEPTSAQTTADAAQSELWADVDHSGYYPELMADSLRATLGSER
                     ALAHVVHQETTLDDSMGIRRHVTVLATTPTRLIVCHTDEHPAGEVTPYPHATTTTEVV
                     PLSRIASVALTTTVANPAEYVPGGSYREATLVVGWGAISHLDLEPATCGDENCEADHG
                     YSGTLTADDLSLRVSQDGDGAPVVAQAIRFATVLSEAAARARA"
BASE COUNT          435 a          832 c          792 g          348 t
ORIGIN      
        1 gataccgtca aggcgctcgc cgagcgccac gcgacgccgc tgtgggtggt cggagggggc
       61 aagaccgccc gcggccatcg cgtgatggtc tcggctcgac gtccccttca ctggatcgat
      121 cacccagtga atgatcaaca catcgccgtg gttttgccgt acgaaccgcg gcgggacgat
      181 ttgctccccg atccggaggc actacgtgag ctcgcgttag ccgaaaatga gctcgtcggc
      241 atccttggag caagcgccga attcgtagcc gccgtaacag ccgaaggtag gcggactctc
      301 catttctacg ccgactccga gaatcctcac cccgctgcgg tgatccgcca gtgggtgcac
      361 gacaacccag aagctcgatg gaccatcgcc gtagacccgg gctggtccgc cgtagcggct
      421 ctcgcgcgcg cacctctcca accacagggc ggcgagtagg gcaagatcgc tcgtcgacct
      481 agccggtgaa gcaggcgagc ggcacgagtt gctcggcggc ggtcagcgat ccgtgatggc
      541 cgatgagccc cgcctcgccc ggatggcggc gcgagtcgac gaccgcgatg tcggcgagcg
      601 cggctacgac gacgtcgccg atccggtctc ggtaaggatc cgacaccaca ccgaaccagc
      661 ctgcctcgat cgcctcatcg cgggtgcaca cgaccgcgtc ggaacccagg cgcgaagtcc
      721 accccgacat cacgtcgtcg agcccgccgg ggcgcacgta gagatagcga gcccgaggct
      781 ctccgcccat cagccggacg ccgtcgagca gccacggctc ggcctcgacg tcaacacgcc
      841 ggctcgcctc gatgtcgacc atgccgtgat cagccgtgac gtagagcacg ctccccgctg
      901 gcagtcgctc gacaatctgc tcgacgagcc gctcgacgag ttgaagctgc aacctccacg
      961 ccaacgaccc gacgccgtgc ccgtggccgg tccagtcgag gtcagcgaca tacgcataca
     1021 cgaggcacgg acgccgagca gtgaccgcct ccttcaactg cgcgagtgcc ccggcgccaa
     1081 cttcaccgaa agtgtcggat cccacgaaag aggccgcccg caacacagct cggtcaagcc
     1141 ccccgccctc aaactcgcgc ttggccacct cgaccacgga cgtgccggcg gtgaccgcac
     1201 gttcgaaggc cgtcggcagc ggctgccact cgaccggatc gacctcggag tcccagcgca
     1261 gcgaattcat caggcgtgcg gtgccgggga tcgcgacttg atagccgagg ataccgtgtg
     1321 cgccgggcgg caagccggtg ccgatcgagg cgatgctgac cgccgtcgtc gaggggaaac
     1381 cggaggtgag atcgcgaccc gacccgccga ccagtgctgc gtgcagcagt ggcgcgtcgg
     1441 cgctgtgtgc cctgaccagc tccgcgccga gaccatcgat caacaggatg caagccgctg
     1501 cagcaggcgc gagcgacagc acgttcgtga aggtcggggt gccgattgac gccagcacgg
     1561 acggcaccag atcggccaga gatccggtcc cgtagcgcgg gatgggcacc gcgcgcgggt
     1621 cgaccaaagt cactggtcag gcccgagccc gcgccgccgc ctcggacaga accgtcgcaa
     1681 acctgatggc ctgggccacc accggagcgc cgtcgccgtc ctggctcacg cgtagtgaca
     1741 gatcgtcagc agtcaacgtg cctgagtacc cgtggtcggc ttcgcagttc tcgtcgccgc
     1801 acgtggcggg ctcgagatca agatggctga tcgcccccca gccgacgaca agagtggcct
     1861 cgcggtacga accgccggga acgtactcgg ccggattcgc gaccgtggtc gtcagcgcga
     1921 cggaggcgat ccgcgacagc ggcacgacct cggtggtcgt ggtggcgtgc gggtacggcg
     1981 tgacctcccc ggccggatgc tcgtcggtgt ggcagacgat caaccgcgtc ggcgtcgtcg
     2041 ccaggaccgt gacgtgccga cgaatgccca tggagtcgtc aagggtcgtt tcctgatgga
     2101 cgacgtgggc gagggcgcgt tcgctgccaa gcgtcgcgcg cagcgaatcg gccatcagct
     2161 cggggtagta gccgctgtga tcgacgtccg cccatagttc tgactgcgcc gcgtcggcag
     2221 tggtctgggc gctcgtgggt tcggccatgg ctccatcctc caccatcgag ggccggggga
     2281 cgtaacaccg cagaaatcga gaagatcgcc gccccgttac gtggcagagt cagcatctga
     2341 aatcagcgtc ggcgcacgga gggtacaaac agatggccac cttcgagacc accacttcag
     2401 gcagccc
//
LOCUS       DASWVF010000819         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_430859, whole genome shotgun sequence.
ACCESSION   DASWVF010000819 DASWVF010000000
VERSION     DASWVF010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430859"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VGD55_14470"
     CDS             <1..1104
                     /locus_tag="VGD55_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0871599.1"
                     /translation="GKTTTTSMLARALQHCGEDPSFAIGADLNEPGSNAHDGGGDTFV
                     AEADESDESFLLLSPFAAIVTNIEADHLNHYAGLAEIHQAFERFAGRIAGDGFLVVCA
                     DDPGAMQLARFARTVGIDVRTYGEAPDADVRIVNLELAGTGSSFHVIADGRRLGEVVL
                     RVPGRHNALNSAAATTIGLGLDMPFAEFAAGLTVFTGARRRFEPKGVVNGVRVYDDYA
                     HHPTEVAAILTAAREVVGAGRLVVAFQAHHYYRTADFCKEFGQALALADEVVVMEVYA
                     PGETMLPGGTGTALAAAVTLAPEHVVFEPSWSAVPAHLAKRARPGDLVLTMGAGGDVA
                     MLAPEVLEALRHVAEHGSGHGGQGSETTRRIPR"
     gene            1284..>2234
                     /gene="ftsZ"
                     /locus_tag="VGD55_14475"
     CDS             1284..>2234
                     /gene="ftsZ"
                     /locus_tag="VGD55_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0871600.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEVGLKGVEFIAINTDA
                     QALLMSDADVKLDIGRELTRGLGAGADPEVGRQAAEDHTEEIEEVLKGADMVFVTAGE
                     GGGTGTGGAPVVARLSRALGALTIGVVTRPFGFEGRRRGEQADVGIEELRDEVDTLIV
                     IPNDRLLSISDRKISVLDAFKSADQVLLQGVSGITDLITTPGLINLDFADVKSVMAGA
                     GSALMGIGSARGEDRAVAAAEMAISSPLLEASIEGAHGVLLSVAGGSDLGLFEINEAA
                     QLVAEAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDGGSP"
BASE COUNT          378 a          695 c          766 g          395 t
ORIGIN      
        1 ggcaagacca cgactacgtc gatgctcgcc cgcgcgttgc aacactgcgg cgaagacccg
       61 tcgtttgcca tcggggccga tctgaacgag ccgggatcga acgcgcatga cggcgggggc
      121 gacaccttcg tcgccgaagc cgacgagagt gatgaatcgt tcctgttgct gtcgcccttc
      181 gcggcgatcg tcacgaacat cgaggccgac cacctgaacc actacgccgg cttggccgag
      241 attcatcagg cgttcgagcg ttttgcgggg cgcatagcgg gcgatggatt cctcgttgtg
      301 tgcgccgatg acccaggcgc gatgcagctc gcgcggttcg cacggaccgt cggcatcgac
      361 gtccggacct atggcgaagc tcccgatgct gacgtgcgga tcgtcaacct tgagctcgcg
      421 gggaccggct cgtcgtttca cgtcattgca gatggacgcc ggctcggtga ggttgtactg
      481 cgcgtgccgg gccgtcacaa cgcgctgaac tctgctgcgg caacgaccat cgggctcggg
      541 ctcgatatgc cgttcgccga gttcgccgcg ggcctgactg tcttcacggg tgcgcggcgg
      601 cgattcgaac cgaagggcgt tgtcaacggg gtgcgggtct atgacgacta cgcccatcat
      661 ccaaccgagg tcgcggcgat cctgacggcc gcgcgcgagg tcgtgggcgc cggccggttg
      721 gtcgtggcgt ttcaagcaca ccactactac cggaccgccg acttctgcaa agagttcggt
      781 caggcgctcg cactcgcgga cgaagtcgtg gtgatggagg tgtacgcccc gggggagacg
      841 atgctgccgg gcggcaccgg gaccgcgctt gccgccgcgg tgacgctggc cccggaacac
      901 gtcgtattcg agccatcgtg gtcagcggtt cccgcgcatc tcgcgaagcg ggcgcgaccg
      961 ggcgatctgg tgttgacgat gggcgcaggg ggagacgtcg cgatgcttgc gcctgaggtt
     1021 ctcgaagccc tgcggcacgt tgcggagcac ggctcagggc acggcgggca aggttcggag
     1081 acgacacgcc ggatcccgcg ttgacccctc gcgggtatgg gctctaatct cctcgcatct
     1141 caggttgaca taactataac ggtctacttg aggatgagag tcgtgggtcg acggtggtgg
     1201 cttccccgca ccatcgctgt gatgcgcaag ctcccacgca cgcacgcatg ctgaaacaca
     1261 cctgggaagg caaggcgaag cccgtggcag caccgcagaa ctacctcgcg gtcatcaagg
     1321 tcgtcggcat cggcggcggc ggtgtcaacg ccgtcaaccg gatgatcgag gtcggtctca
     1381 agggtgtcga gttcatcgcg atcaacaccg acgcgcaggc gctgctgatg agcgacgctg
     1441 acgtgaagct cgacatcggt cgcgagctca cccgcggtct tggcgccggc gccgaccctg
     1501 aggttggccg gcaggccgcc gaagaccaca cagaggaaat cgaagaggtg ctcaagggcg
     1561 ccgacatggt gttcgtgacc gctggcgagg gtggcggcac cggcaccggt ggcgctccgg
     1621 ttgtcgcccg actgtcccgc gccctcggtg ctctcaccat cggagtcgtg acgcgtccct
     1681 tcgggttcga aggtcgtcgt cgcggcgagc aagccgatgt gggcatcgaa gaacttcgcg
     1741 atgaagtcga caccctgatc gtcattccga acgaccggct gctctcgatc agtgaccgca
     1801 agatcagcgt gctcgacgcg ttcaagtctg ccgatcaggt gctgctgcag ggcgtgtctg
     1861 gcatcaccga tctcatcacg acgccgggcc tcatcaacct cgacttcgcc gacgtgaagt
     1921 ccgtgatggc cggagcaggt tctgcgttga tgggcatcgg ctcggcgcgc ggtgaagacc
     1981 gcgcagtcgc ggcagccgag atggcgattt cgagcccgct gctcgaagcg tcgatcgaag
     2041 gcgcacacgg tgtgctgctt tcggtagcag gtggttccga tctcgggctg ttcgaaatca
     2101 acgaagccgc gcaactcgtc gcagaagcgg ctcaccccga tgccaacatc attttcggtg
     2161 ccgtcatcga cgatgcgctt ggcgacgagg tcagggtgac cgtgatcgcg gcggggttcg
     2221 acggcggatc gccg
//
LOCUS       DASWVF010000820         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_303311, whole genome shotgun sequence.
ACCESSION   DASWVF010000820 DASWVF010000000
VERSION     DASWVF010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303311"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..658)
                     /locus_tag="VGD55_14480"
     CDS             complement(23..658)
                     /locus_tag="VGD55_14480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0871601.1"
                     /translation="MSTTSVDTVDTEQPPGELVLLRHGATTWSASGKHTGRTDVPLSS
                     DGEQQAARVADLIADRNFAMVLASPLVRAQETAQLAGLSHIETDANLVEWDYGGYEGL
                     TTAQIRAILPGWSLWRDGVPAHSDGQPGENAADVGVRADRVIARVLPVLGRGDVALVS
                     HGHFLRVLAARWLSLAPTGGAMLALDTGSVSVLGFEHGAHVLRHWNLLPPP"
     gene            complement(655..1956)
                     /locus_tag="VGD55_14485"
     CDS             complement(655..1956)
                     /locus_tag="VGD55_14485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0871602.1"
                     /translation="MATSTAIPRDASQPRVNLDVLALAAPVLALLVVAIIRKGAFFRP
                     DIVVSPALTLALALSVPQVREWARDHLEAVGAGLLATAWWIGDAELSHHSIDSWRLPA
                     AWLCLASGYGCARSLAPAARRVALIGTAVVGDALAIAGLALVGVRSTFWTLQDQRSLR
                     FAGPFTYPSAVGLFLVISLLAGTGIEANSRVRRVLNCVRAVTVLGIVSTDSRGAVVGL
                     AVALCFRSVRRELGTPVVAAVIGAPLLLLGQRTHTSHLLVVAAVIVAAGLALIPDGVV
                     RRAVRFVAVPAVCVVGWLLVTQHHAVSGFDGSWTERGHILRGAGHVFAKHPLFGAGPD
                     PSFGANTLTGQAGIAYFTHNEPVEILISVGVVGTVVLVLCAVVVGRALAAGRGSLAVP
                     VLVSVGAAGLVDFVWHFPALGLATGIVAGASGLRRGSNVAP"
     gene            complement(2017..>2337)
                     /locus_tag="VGD55_14490"
     CDS             complement(2017..>2337)
                     /locus_tag="VGD55_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0871603.1"
                     /translation="LGMVMGTCVYHIAHRGLGNVLSYVGKNTELTQFTQALYDARELA
                     MERMQKEAEELHAEGIVGVQLHQRNHSWGAHTTEFFSIGTAVRPLRADHVITPPSMVV
                     SLDN"
BASE COUNT          431 a          778 c          795 g          333 t
ORIGIN      
        1 cggcgaggac gacaagaggt agctagggcg gcggcagcag gttccagtgc cgcagcacgt
       61 gggcgccgtg ctcgaagccg agtaccgaca cgctgcccgt gtcgagcgcg agcatcgccc
      121 cgcccgttgg cgctagtgaa agccagcggg cggcgagcac gcgcaggaaa tggccgtgcg
      181 aaaccagtgc gacatcgcct cgaccgagca cgggcaatac gcgcgcgatc acgcgatcgg
      241 cgcgcacccc gacgtccgcc gcgttctctc cgggctgacc gtcgctgtgg gcgggcacgc
      301 cgtcgcgcca cagcgaccag ccgggcagga tcgctcgaat ctgcgccgtg gtcaggcctt
      361 cgtagccgcc gtagtcccac tcgacgaggt tggcgtcggt ctcgatgtgc gacagtccgg
      421 cgagttgcgc agtctcttgc gcgcgaacta acggactcgc aagaaccatc gcgaagttgc
      481 ggtcggcgat gaggtcggcg actcgcgccg cctgctgctc accatccgaa ctcagcggga
      541 cgtcggtgcg acccgtgtgt ttgccactcg cgctccacgt ggtggcaccg tgccgcagca
      601 gcacgagctc gccgggaggc tgctcagtat cgacggtgtc gaccgacgtg gtgctcacgg
      661 cgccacgtta ctgccgcgcc gcaagccact cgcgccggcc acgatcccgg tcgcgaggcc
      721 tagtgcgggg aagtgccaca cgaagtcgac gagccccgcg gcaccgacgc tgacgagcac
      781 gggcactgcc agtgatccgc ggcctgcggc aagtgcgcga ccgacgacca cggcgcacaa
      841 cacgagcacc accgtgccga ccactccgac cgaaatcagg atctcgacgg gctcgttgtg
      901 ggtgaaatac gcgatgcctg cttggcccgt caacgtgttc gcgccgaagc tcgggtcagg
      961 ccctgcgccg aagagtgggt gcttcgcgaa gacgtggccg gccccgcgca ggatgtgacc
     1021 gcgttcggtc cagctcccgt cgaaacccga gacggcgtgg tgctgagtca cgagtaacca
     1081 gccgacgacg cacaccgccg ggacagcgac gaagcgcacg gcgcgacgca ccacgccgtc
     1141 gggtatgagg gcaagacccg cagcaacaat gaccgccgcg acgacaagga gatgactcgt
     1201 gtgcgtgcgc tgacccagca acagcaacgg cgccccgatc actgcggcaa cgactggagt
     1261 accgagttct cgccgcacgc tgcggaagca caacgcaacc gcgagcccga ccacagcacc
     1321 gcgactgtca gtcgacacga tgccgagaac cgtgaccgcc cgcacgcagt tgagaacacg
     1381 gcgcactcgt gagttcgcct cgatacccgt acccgcgagc agcgaaatca ccaggaacag
     1441 gccaactgcg ctcgggtaag tgaagggtcc cgcgaagcgc agcgaacgct gatcctgcaa
     1501 ggtccaaaac gtcgagcgga cgccgaccag tgcaagtccg gcgatcgcca gtgcgtcgcc
     1561 gacaactgcc gtgccgatga gcgccactcg gcgcgcagcc ggggcgaggc tgcgcgcgca
     1621 gccgtatccg gacgccaggc acaaccacgc ggccggcagc cgccacgagt cgatgctgtg
     1681 gtgcgagagt tcggcgtcgc cgatccacca cgcggtggcg agcagcccgg cgccgactgc
     1741 ttcgaggtga tcacgagccc attcgcgcac ctgcggtaca gacagcgcga gggcgagggt
     1801 aagtgccggc gagacgacga tgtcagggcg aaagaaggcg cccttgcgga tgatcgccac
     1861 gactagcaac gcgagcaccg gcgccgccaa cgcgagtacg tcgagattga cgcgaggctg
     1921 gctggcgtct cgcgggatcg cggtactggt ggccaccgtc gttgtcgagg ctctggtttg
     1981 tcgaagctct ggtgtcgaag ctctggtgtc gaggtgctag ttgtcgaggc tgacgaccat
     2041 ggacggcggc gtgatgacgt ggtcggcgcg cagtggtcga accgccgtac cgatcgagaa
     2101 aaactccgtc gtgtgggcgc cccaagagtg gttgcgctgg tgcaactgca caccgacgat
     2161 gccctcggcg tgcagttctt ccgcttcctt ctgcatgcgt tccatcgcga gttcgcgtgc
     2221 gtcgtagagc gcctgcgtga attgcgtgag ctcagtgttc ttgccgacgt acgacagcac
     2281 gttgccgagt ccgcgatgcg cgatgtggta gacgcaggtg cccatcacca tgccgag
//
LOCUS       DASWVF010000821         5078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_303316, whole genome shotgun sequence.
ACCESSION   DASWVF010000821 DASWVF010000000
VERSION     DASWVF010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303316"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VGD55_14495"
     CDS             <1..84
                     /locus_tag="VGD55_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075786.1"
                     /note="Involved in peptide bond synthesis; alters the
                     affinity of the ribosome for aminoacyl-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0871604.1"
                     /translation="QVPLFITTGEKVKVDTRTGDYLGRINS"
     gene            129..539
                     /gene="nusB"
                     /locus_tag="VGD55_14500"
     CDS             129..539
                     /gene="nusB"
                     /locus_tag="VGD55_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0871605.1"
                     /translation="MSARTKARKRALDVLFECDLRDSDALETLARQRAQATPVVPDYT
                     VTLVEGVVTHRSRIDELIESYAVGWTLDRMPVVDRNLLRIGAFELLWSDEVPDGVVLS
                     EAVNLARDLSTDDSPTFVNGVLARILEEKPSLVV"
     gene            648..1994
                     /locus_tag="VGD55_14505"
     CDS             648..1994
                     /locus_tag="VGD55_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0871606.1"
                     /translation="MDVIAELRSELPAGRLLTDIDVVEAYRRDEARMVEPGRPLGVVL
                     AHDVGDVQATLRWATRRRVPIVARGAGTGLSGGATAIDGCLVLSLAKMNAIVEMNADD
                     QLAVVEPGVINSDLQNAAAEHGLMFAPDPASFEISTIGGNVATNAGGLRCVKYGVTRE
                     AVLGLEVVLADGRVMKTGRRTVKGVAGYDLTALFVGSEGTLGIVTQATVRLRPKPRAD
                     PVTIVGVFATLTSAGDAVNDVIGAGLQPSLLELMDNATIRAVDDWKHVGLDRDAAAML
                     IAQVDIDHPREPALAIEKAFAAAGATYTAVSTDATEAAELLGIRRMTHWAIERLGMIL
                     TEDVGVPRSKLAQMLARIEEIGARYDVVIATVGHAGDGNLHPSIVVDSDRAQQAAEEI
                     YKAALELGGTITGEHGIGSLKRDWLPAEIGDVGMDVQRALKDTFDPLGILNPGKAI"
     gene            complement(1991..2482)
                     /locus_tag="VGD55_14510"
     CDS             complement(1991..2482)
                     /locus_tag="VGD55_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0871607.1"
                     /translation="MNDYAKALGARLRAIRTQQGLSLHGVEEKSRGRWKAVVVGSYER
                     GDRAVTVQRLAELADFYGVPVGELLPDATPSGASEPPPRLVIDLEQLADVPPEKARPL
                     ARYAATIQSQRGDYNGRVLSIRQDDLRSLAVIYDESPSLLTEELISWGVLNAEARRAV
                     ETL"
     gene            2801..3385
                     /gene="pyrR"
                     /locus_tag="VGD55_14515"
     CDS             2801..3385
                     /gene="pyrR"
                     /locus_tag="VGD55_14515"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY0871608.1"
                     /translation="MTAAQHSSDTPSVPPRVVLDAADMARALTRIAHEILERTKGGAD
                     VVLLGIPTRGVGLAQRLARQMSAVEQREVLAGSLDITMYRDDLRLRPPRALARTEVPG
                     GGVDGRLVVLVDDVLYSGRTVRAALDALGELGRPRAVQLAVLVDRGHRELPIKADYVG
                     KNLPTSAAEQVTVLIEELDGRDAVLLGPTAEVTS"
     gene            3382..4371
                     /locus_tag="VGD55_14520"
     CDS             3382..4371
                     /locus_tag="VGD55_14520"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0871609.1"
                     /translation="MTRHLLSAGDLSRDDAMLILDTATELARLSDRPIKKLPTLRGRT
                     VVNLFFEDSTRTRTSFEAAAKRLSADVINFSAKGSSVSKGESLKDTALTLEAMGADGV
                     VIRHEASGAPHRLAGWIKGSVVNAGDGTHEHPTQALLDAFTMRRHLGNLDGRRVAIVG
                     DVLHSRVARSNVLLLRTLGAEVTVVAPPTLLPVGVDGWPCAHSYDLDTVLPKADVVMM
                     LRVQRERMNASYFPNAREYSRRYGLDSARMAVLPDHAIVMHPGPMNRGMEIAADVADS
                     ERSVIVEQVTNGVSVRMAVLYPLLGGSESAIGAESDRASAIDAETEHATAEAL"
     gene            4368..>5078
                     /locus_tag="VGD55_14525"
     CDS             4368..>5078
                     /locus_tag="VGD55_14525"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0871610.1"
                     /translation="MSSWLLKGVSISGGEPIDVLLDEGVVGEIGAGLTHRGATVIDAD
                     GLIALPGLVDLHTHLREPGREDAETVETGTLAAALGGFTAVHAMANTDPVADSAGVVE
                     QVWLAGRDAGHCDVHPVGAVSVGLHGQQLAELGAMADSHARVRVFSDDGKCVSDALLM
                     RRALEYVKAFDGVIAQHAQEPRLTEDAQMNESELSGVLGLRGWPAVAEEAIIARDILL
                     TEHVASRLHICHISTAGSV"
BASE COUNT          890 a         1684 c         1676 g          828 t
ORIGIN      
        1 caggtgccgc tgttcatcac aacgggtgag aaggtcaagg tcgacacccg cacgggcgac
       61 tacctcggcc ggatcaacag ctgacgcgtg cacaacccag ctctagatct aaatccgatc
      121 ggacgccgat gagcgcgcgc acaaaggccc gcaagcgcgc tctcgacgtg ctgttcgaat
      181 gcgatctgcg tgactccgat gccctcgaaa cgctggcccg gcaacgcgcg caggcgaccc
      241 cggtcgtccc cgactacacc gtgaccttgg tcgaaggggt cgtcactcac cgctcgagga
      301 tcgacgagct cattgagtcg tatgcggtcg gctggactct cgaccgtatg ccggtggtcg
      361 accggaatct gctgcggatc ggcgccttcg agttgttgtg gtccgacgag gtgcccgacg
      421 gagtcgtgct cagcgaagca gtcaacctgg cgcgcgacct gtcgaccgac gactcaccga
      481 ccttcgtgaa cggcgtgctc gcgagaatcc tcgaagagaa gccgtcgctc gtcgtctgac
      541 gccgcaacgg gcaccacccg ccgtccatca ttgtggagtc ctggcgctgc tagagcactg
      601 cgagaactcc acgatcccac gtgcggctgg cgggttaccc tcgacgcgtg gatgtgatcg
      661 ccgaactgcg cagcgagttg ccggccgggc gcctgctgac cgatatcgac gtggtcgagg
      721 cctaccggcg cgacgaagcg cggatggtcg agccggggcg gccactgggc gtggtcctgg
      781 cgcatgatgt tggcgatgtg caggcgacgc tgcgctgggc aacgaggcgt cgcgtcccca
      841 ttgtcgccag gggagcgggc accgggttgt caggtggggc gacggcgatc gacggctgtc
      901 tcgttctctc actggcgaag atgaacgcga tcgtcgagat gaacgccgac gatcagcttg
      961 cggtggtcga gccgggcgtc atcaactccg acttgcagaa cgccgcagcc gagcacgggt
     1021 tgatgttcgc ccccgacccg gccagctttg agatctcgac catcggcggc aacgtcgcga
     1081 ccaacgccgg cggcttgcgc tgcgtgaaat acggcgtcac gcgcgaggcg gtgctcgggc
     1141 tcgaggtcgt cctcgccgac ggtcgggtca tgaagaccgg acgacgcacc gtcaaaggcg
     1201 ttgccggcta cgacctcaca gcgttgttcg tcggctccga gggaacgctg ggcatcgtca
     1261 cgcaagccac cgtgcggttg cgaccaaagc cacgcgccga cccggtcacc atcgtcggcg
     1321 tgttcgcaac cctcacctca gctggcgacg cagtgaacga cgtcatcggt gccgggctac
     1381 aaccgagcct gctcgaactg atggacaacg cgacgatccg cgcggtcgac gactggaagc
     1441 acgtcggtct cgaccgcgac gccgccgcga tgctgatcgc gcaggtcgac atcgaccatc
     1501 cgcgcgaacc ggcgctcgcg atcgagaagg cgtttgccgc cgctggtgcc acctacaccg
     1561 cggtctcgac cgacgcgacc gaggcagccg aactccttgg catccggcgc atgacgcact
     1621 gggcgatcga gcggctcggc atgatcctca ccgaggacgt cggcgtgccc cgatcgaagc
     1681 tggcgcagat gctggcaagg atcgaagaga tcggtgcgcg ctacgacgtg gtgatcgcca
     1741 ccgtcgggca tgcgggcgac gggaacctgc accccagcat cgtggtcgat tccgaccgtg
     1801 cgcagcaggc cgccgaggag atctacaaag cggcgctcga actcggcggc accatcacgg
     1861 gtgagcacgg catcggcagc ctgaagcgtg actggttgcc ggctgagatc ggagacgtgg
     1921 gcatggacgt gcaacgcgcg ctgaaggaca cgttcgaccc gctcgggatt ctcaacccag
     1981 gcaaggcgat ctagagggtt tcgaccgccc ggcgagcctc ggcgttcaag acgccccacg
     2041 agatcaactc ttcggtcagc aggctcggcg attcgtcata gatgacggcc agcgaacgca
     2101 gatcgtcctg ccgaatcgag agaacccggc cgttgtagtc gccgcgctgg ctctggatcg
     2161 tcgccgcata ccgcgcaagc ggacgagcct tttccggcgg gacgtcggcc agttgctcta
     2221 ggtcgataac caaccgcggc ggcggctcgc tcgcgccact cggggtcgcg tcgggcagca
     2281 actcgcccac cgggacgccg tagaagtcag caagttcggc aaggcgctga accgtcactg
     2341 cccggtcgcc gcgctcgtag gaaccgacga cgacagcctt ccagcggccg cgcgacttct
     2401 cctcgacccc gtgcaacgac aggccctgct gggtgcggat cgctcgcagc ctggcgccga
     2461 gcgccttggc gtagtcattc acggtcgttc accgtcatgt actgggtcct cgcagctggg
     2521 ggtgcagttc ggcaaaccgg gcggcctgca gcgggtggag cctcacacct ggtcactacc
     2581 gggcgccggt ttcaccagtg gctacttcgg gggatgcccc cgaaagggac atagtgacct
     2641 gaagatcccg aagtcatagc cgcgctcgtt actcattgtg acggtaggcc catcgttact
     2701 ctgcgtcaag cagcttgtga ccgcgagcgt cctgctaggg tttgccgtgc tcgacatcct
     2761 ttaagacccg tccggtgagg cggggaagga ggtccaggcg atgacggctg ctcagcacag
     2821 ctcagacacg ccctcagttc cgcctcgcgt ggtgcttgac gcggccgaca tggcccgcgc
     2881 actgacgcgg attgcgcacg aaatcctcga acgcaccaag ggtggggcag acgtcgtgct
     2941 gctcggaatc ccgacccggg gcgtcggcct ggcacagcga ctcgcccgcc agatgagcgc
     3001 ggttgagcag cgcgaggtgc tggctggctc cctcgacatc accatgtacc gcgacgacct
     3061 gcggctgcgc ccgccgcgcg ccctcgccag aaccgaagtg cccggtggcg gagtcgacgg
     3121 caggctcgtc gtcctcgtcg acgatgtctt gtactcaggt cgcacggtgc gtgccgctct
     3181 cgatgcgctc ggcgagctag gtcgcccgcg cgccgtgcag ttggcggtgc tcgtcgatcg
     3241 cgggcatcgc gagctgccaa tcaaggccga ctacgtcgga aagaacctgc caacatcggc
     3301 cgccgagcaa gtcacggtgc tcatcgaaga actagacggg cgggacgccg tgttgctcgg
     3361 gccgaccgcc gaggtgacct catgaccaga catctgctct cagccggcga tctcagtcgt
     3421 gatgacgcga tgctgatcct cgacaccgcc accgagttgg ccaggctttc cgaccgaccc
     3481 atcaagaagt tgccgacact tcgtggtcgc accgtggtca atctgttctt cgaagactcc
     3541 acccgcaccc gcacctcgtt cgaagctgcg gcgaaacggc tatccgccga cgtcatcaac
     3601 ttctccgcca aagggtcgag cgtgtcgaag ggcgagagcc tgaaagacac cgcactgaca
     3661 ctggaagcaa tgggcgccga cggcgttgtc atcaggcacg aggccagcgg cgcgccgcac
     3721 cggctcgccg ggtggatcaa aggcagcgtc gtgaacgccg gcgacggaac ccacgagcac
     3781 ccgacccagg cgctgctcga cgccttcacg atgcggcggc acctcggcaa tctcgacgga
     3841 cgcagggtgg cgatcgtcgg tgatgtgctg cacagccgcg tcgcgcggtc gaacgtgttg
     3901 ctgctgcgca cactcggtgc cgaggtcacc gtcgtcgcgc caccgaccct gcttcccgtc
     3961 ggagtcgacg ggtggccgtg cgcgcactcg tacgacctcg acaccgtgct gccgaaggcc
     4021 gacgtggtga tgatgctgcg cgttcagcgc gagcgcatga acgcgtcgta tttccctaat
     4081 gcacgcgaat acagccgccg gtacggtctc gactcggcac gcatggccgt gttgcccgat
     4141 catgcgattg tcatgcatcc cggcccgatg aaccgtggca tggaaattgc ggccgacgtt
     4201 gccgactccg aaagatcggt gatcgtcgag caggtgacaa acggtgtcag cgtgcgcatg
     4261 gcagtgctct accccctgct cggcggcagc gaatcggcca tcggcgccga gagcgaccgg
     4321 gcctcggcca tcgatgccga gaccgagcac gcaacggcgg aggcactatg agcagctggc
     4381 tcctcaaggg cgtctcgatc agcggcgggg agccgatcga cgtcctgctt gacgaaggtg
     4441 tcgtcggtga gatcggtgct ggcttgacgc atcgcggcgc gaccgtcatc gatgccgacg
     4501 ggctcatcgc actgcctggc ctcgtcgacc tgcacacgca cctgcgcgag cccggccgcg
     4561 aagacgccga aaccgtcgag accggcacct tggccgcagc ccttggtgga ttcactgccg
     4621 tgcatgccat ggcgaacaca gaccccgtcg ccgactctgc cggtgtggtc gagcaggtgt
     4681 ggctggccgg tcgcgacgcc ggccattgcg acgtccaccc ggttggcgcg gtgagcgtcg
     4741 gcctgcacgg acagcagctt gctgaactcg gcgctatggc ggattcacat gcccgcgtaa
     4801 gggttttctc cgacgacggg aagtgcgtct ccgacgcatt gttgatgcgc cgggcgcttg
     4861 agtacgtcaa ggcgttcgac ggcgtcatcg cccagcacgc acaagagccg cgccttacag
     4921 aagacgctca gatgaacgag agtgagctgt ctggcgtgct cggcctgcgg ggctggccgg
     4981 ccgtcgccga agaagcgatc atcgcgcgcg acatcttgct gacagagcac gtcgcatccc
     5041 gcttgcacat ctgtcacatc tccacagcgg gatccgtc
//
LOCUS       DASWVF010000822         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_48678, whole genome shotgun sequence.
ACCESSION   DASWVF010000822 DASWVF010000000
VERSION     DASWVF010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48678"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..380
                     /locus_tag="VGD55_14530"
     CDS             258..380
                     /locus_tag="VGD55_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871611.1"
                     /translation="MAVEAPPADDYVAKPADRCKIQPNNRLRGYLLFGTRLVAV"
     gene            967..1359
                     /locus_tag="VGD55_14535"
     CDS             967..1359
                     /locus_tag="VGD55_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871612.1"
                     /translation="MIGLLLVGLDIARPVTFNGLGGVVFAFPALIAYMCLVNLALSRR
                     HLRMFVLDNVAFCAVLWGLVYGQYESVLWARLTAFGLAACAVALAIPLRSQRPAPATQ
                     LPKNLRQAGLQPKSASDASIGMAPDVEQ"
     gene            1346..1978
                     /locus_tag="VGD55_14540"
     CDS             1346..1978
                     /locus_tag="VGD55_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871613.1"
                     /translation="MSSSRRFTVLVAILAVGVAVTSCTSMSQDAAPALKSIAVGPNGS
                     IPAYESAAVASLTTQQNLREFLPCAVGKRTITVGETYPEKDTAELSVTLTSHLTGQGA
                     GAPGPKSGEYEVASIQITGRAGSYAYSPSQFAFITPKGELVRSTDGNSPAAGFEPPLG
                     SGSVTKGQLVVGTLTFDVPRGGGGLVFSDVIFPDGNKDLLGGRCTWVIGT"
     gene            complement(2132..>2351)
                     /locus_tag="VGD55_14545"
     CDS             complement(2132..>2351)
                     /locus_tag="VGD55_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871614.1"
                     /translation="HMSKRPAAFIPAGVLGTVIMSLIVAFLVPHHTTSAFFVHLLLIG
                     AVVGSVEVVAGMVIIAFALRKTGLPPSG"
BASE COUNT          423 a          741 c          758 g          429 t
ORIGIN      
        1 gaccggacgg atgatccccg aaagcgcaac gaccaccggt tcaagctgga cggagggctg
       61 ggtctcgcgt tcacaacgat catccaagat gcggctgatg ccttcgtctt ccatcatccg
      121 atgcacgatc cctttgcagc ccaccgttca gtgagcccgg gcctgccgag gccatggagt
      181 ggcgaccagc gaacggacca gcccgagtat tacgtgtcgt atcgggtgtt gagtccagct
      241 ctcaccatcg aacgcgaatg gctgtcgaag caccccccgc cgacgactac gtagctaagc
      301 ccgctgaccg gtgcaagatc cagccgaaca accggcttcg cggctacttg ttgttcggaa
      361 cccgtctggt ggcggtgtga cgcgccttgg atgccgtcgg gggttccgac ggacggaagg
      421 ggatgccatc gcgcttgtgg gccgtagcat cggggcgtgg gatacgggtg ggggattgcg
      481 gcgcgagtgt ctgtgcccat cggtgcattc ggcgggttgc tgtggcggac aggatctgcg
      541 aatcggtcga gttgcacgct gcccaatgag ctcggtcgcc gtttcgtgcc ggatctgcaa
      601 aacgactgcc agtgggcgaa gtcggacagc cgtgtcggct ttgttgtctt ggtgctggcc
      661 attgtggtgt tcgccgtgtc gttcggcccg gctcgaaagg cgtcccgaca gcatcgggct
      721 cagctcgcgg cggcgccgcc ttcgcctccc ccggggtgga tgcgtgttcc ggtcggctgg
      781 cgaccgacaa atggccgttt catgccagtg ccacctggct ggccgcaacc atctgcggac
      841 tggacggcac ccaaggattg ggagccgcca tcgacgtggc ccgatcctcc gcgcgggtgg
      901 ccgcgaccag atcaccgatc ggcggctgaa gtccgacggt tctgggcagt agcgcgcgcg
      961 tcctgcgtga tcgggttgtt gctcgtgggg ttggacatcg cccgaccggt cacgttcaac
     1021 ggtttgggag gcgtcgtgtt cgcgttcccg gccctgatcg cgtacatgtg cttggttaac
     1081 ctcgcactgt cgcgtcgcca tcttcgaatg ttcgtgctcg acaacgtcgc gttttgcgcc
     1141 gtcctttggg gtttggtcta cgggcagtat gaatcggtgc tgtgggcgcg tctcacggcg
     1201 ttcggactcg ccgcgtgcgc ggtcgcgttg gctatcccgc ttcgctcgca gcgaccggct
     1261 ccagcaacac aactcccgaa gaacctgcga caggcagggc tccagcccaa atcggcgtcc
     1321 gacgcatcga tcggaatggc gccagatgtc gagcagtaga cgattcaccg tgctggttgc
     1381 gatcttggct gtcggcgtag cagtcacgag ctgcacatcg atgagccagg acgccgcgcc
     1441 cgcgttgaaa agcatcgcgg tcggaccaaa cggctcgata cccgcctacg aaagcgccgc
     1501 cgtcgcatca ttgacgacac agcaaaacct gcgtgagttc ctgccatgcg cagtcggcaa
     1561 gaggacgatc accgtgggtg agacctaccc cgagaaggac accgcggagc tgtccgtcac
     1621 cctcacctca cacctgaccg gacaaggcgc tggcgcaccc ggcccgaagt ccggcgaata
     1681 tgaggtggcc tccatccaga tcacaggacg cgccggctca tacgcctaca gcccgtcgca
     1741 attcgcgttc atcaccccga aaggcgaact cgtccgctcg accgacggga acagcccggc
     1801 cgccgggttc gaaccgccgc tgggaagcgg cagcgtcacc aaggggcaac tggtagtagg
     1861 aaccctgacc ttcgacgtcc cacgaggcgg tggcggcctc gtgttcagcg atgtcatatt
     1921 ccccgatgga aacaaggatc tgctgggcgg acgatgcacc tgggtcatcg gcacctaacg
     1981 ctcgaccgca ggcagtttct cgcactcccg ccgtgagggt gagtgtcgag accgacatca
     2041 agcggcatcc gctcatcggc acgaccgaac gcacgtcaac gcggcagaag agtgtggtca
     2101 aatcttgagc tccaggtttg cgccgagcgc gtcaacccga cggcggcagg cccgtctttc
     2161 tcagggcgaa cgcgatgatc accattccgg caactacctc gacggacccg acgacggcgc
     2221 cgatcagtag aaggtgcacg aagaaggcgc ttgttgtgtg gtgtgggacc agaaacgcca
     2281 cgatcaaact cattatgact gtgccgagga cacccgccgg aataaacgcg gcgggccgtt
     2341 tcgacatgtg c
//
LOCUS       DASWVF010000823         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_99915, whole genome shotgun sequence.
ACCESSION   DASWVF010000823 DASWVF010000000
VERSION     DASWVF010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99915"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="VGD55_14550"
     CDS             <1..2155
                     /locus_tag="VGD55_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0871615.1"
                     /translation="AQLSPRATVCLALDGTVEWANQAFADLVGRTPASVAGAAVGTVL
                     DASTADVLLRWRDELIAGSATAWDADHPLGAAGRRPITMNMSFMALVGPDNSPIGLVG
                     RIDDVSLDRSAAVTLARQTEALDRLARGESVPDVLAALVEAVERDQSGMRCAVLLLDE
                     ADGGRLFTAAAPSLPATFSDIIDGTAIGLHAGSCGRAAFTRRDVVTADIAGDPDWAPW
                     RELALAHGVRACWSVPLMSSGREGDPDKVLGTFVAYFDTPRRPTAQERRDIARTTTLA
                     TIALEMQHDEAELRRANLRDPLTGLANRRLFIDELGAALRRARRRRTDVTVLFLDIDH
                     FKVFNDSMGHLVGDEVLRIVANRVQQAVRPHDVVARFGGDEFTIACEGLGGAAEAEDL
                     AEQFRQAIEGPVQVGDREAFLTVSIGIARSDGWESPRDLVANADAAMYQAKASGRAGV
                     STFDEGMRAQSRDRLHMESALRRAIDKAELRLVYQPQVDLASGQLVGVEALLRWDSEE
                     LGAIAPAEFIPVAEETDLIMGIGEWVLQEACTQAGRWQESRGWSPLVSVNASARQFGR
                     PSVFLENVTSALGASGLEPGQLRIEVTESTLGDPATSGPLVARLKGLGVSLSIDDFGT
                     GFSSLARLRSFPVDELKIDRSFVEGLETQADDRAVVAAVIGMAKALHLDTVGEGVETV
                     GQLDILRTLGCTRVQGYYLGRPMPAHDITPLLSSR"
     gene            complement(2172..2930)
                     /locus_tag="VGD55_14555"
     CDS             complement(2172..2930)
                     /locus_tag="VGD55_14555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0871616.1"
                     /translation="MKRDAAYFDQWYADMESSPTRDALIARILSLPSGLESTGLLSPH
                     AIDEVTTALHLRDDALVLDVACGRGGYGIEIARRTGSRLIGIDFSAVAVEQARRRTAR
                     LELPTGRAQFHVGALDATGLPAGIGDGLMCIDSVQFAEPPLSAMVEFRRLLRPGGRLV
                     MTCWEASDRSDNRVPPRIKAVHLDRDLSAAGFVDVQVRDKADWRAVERTVWQAAVAAP
                     ADSDPAMQSLQAEGRRSLDTFDALRRVFATATAP"
     gene            complement(3007..4116)
                     /locus_tag="VGD55_14560"
     CDS             complement(3007..4116)
                     /locus_tag="VGD55_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY0871617.1"
                     /translation="MAEVQIGMGKMARRAYGFDDVAIVPSRRTRDPEEVSVAWQIDAY
                     RFEAPIVSSAMDSVVSPDTAIEIGRLGGLGVLDLEGLWTRYDDPVPLLDEIATLAEPD
                     VVRRMREIYAEPIKEELIGARIKQIRDGGVVTAAALSPQRTAQYHKAVLEAGVDLFVI
                     RGTTVSAEHVSGRAEPLNLKKFIYDLDVPVIVGGSYTYQAALHLMRTGAAGVLVGVGG
                     GAAHTTRQVLGIGVPMATVVSDAAAARRDYMDESGGRYVHVIADGAMRTGGDIAKAIA
                     CGADAVMIGLPLARASEAPGRGWHWGAEARHTELARGQRYHAGTAGSLREILYGPSTD
                     SAGTTNLVGALRKAMATCGYSDLKEFQRVEVVVSS"
     gene            complement(4141..>4824)
                     /locus_tag="VGD55_14565"
     CDS             complement(4141..>4824)
                     /locus_tag="VGD55_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0871618.1"
                     /translation="RDVTVDVIGGNIATREGAQALVDAGADGVKVGVGPGSICTTRVV
                     TGVGVPQVTAIHEAAQACRAAGVPVIGDGGLQYSGDIAKAIAVGADAVMLGSLLAGVE
                     ESPGELIFINGKQYKSYRGMGSLGAMQSRAEARSYSKDRYFQDDVLSDDKLVPEGIEG
                     QVPYRGPLAAVVHQLVGGLRSAMGYCGAHTIEQLQQARLIRITSAGLKESHPHDVHMT
                     TEAPNYHSR"
BASE COUNT          804 a         1642 c         1569 g          809 t
ORIGIN      
        1 tgcccagctg agtccgcgcg caacagtgtg tctagcgctc gacggcaccg tcgaatgggc
       61 gaaccaggcg ttcgccgatc tcgtcggccg cacgccggcg agcgtggccg gcgcagcggt
      121 tggcacggta ctcgacgcct ccacggccga cgtcttgctg cgatggcgcg acgaactcat
      181 cgcgggtagc gcaaccgcat gggacgccga tcacccgctg ggcgcagccg gtcgtcgtcc
      241 catcacgatg aacatgtcgt tcatggcctt ggtcgggcct gacaactcac cgatcgggct
      301 ggtcggccgc atcgacgacg tgtcgctgga tcgatccgcg gccgtgacgc tggcgcggca
      361 aacagaagca ctcgaccggc tcgcgagagg cgagtcggtg cccgacgtgc tggcggcgct
      421 ggtcgaagcg gtcgagcgcg atcagagcgg catgcgctgc gcggtactgc ttctcgacga
      481 ggccgatggc ggacgcctgt tcacggccgc ggcgccgagc ctgcccgcta cgttctccga
      541 catcatcgac ggcactgcca tcggcctgca tgcgggatcg tgtggccggg ccgctttcac
      601 tcggcgcgac gtggtgactg ccgacatcgc aggggatccc gactgggcgc cgtggcgcga
      661 gctcgccctg gctcacggag tacgcgcctg ctggtcggtg ccgctgatgt cgtcggggcg
      721 cgaaggtgac cccgacaagg ttctcggcac gttcgtcgcc tatttcgaca ccccgcgaag
      781 gccaaccgcg caggagcgac gcgacatcgc gcgcacgacg acgttggcga cgatcgcgtt
      841 ggagatgcag cacgacgaag ccgagctacg gcgtgcaaat ctgcgcgacc cgctgacagg
      901 ccttgctaat cgcaggctct tcatcgacga actcggtgcg gcattgcgac gtgcacgccg
      961 gcgccgcact gatgtcaccg tgcttttcct cgacatcgac cacttcaagg tcttcaacga
     1021 ctcgatgggc catctcgtcg gcgacgaagt actgcgcatc gtggcgaacc gtgtgcagca
     1081 agcagttcgc ccccacgacg tcgtcgcgcg gttcggcggc gacgagttca ccatcgcgtg
     1141 cgaaggtctc ggcggcgcag ccgaagccga agacctcgcg gagcaattcc gccaagcaat
     1201 cgaaggtcca gttcaagtcg gtgatcgtga agcgttcctc accgtcagca tcggcatcgc
     1261 ccgcagcgac ggctgggaat ccccgcgtga tctcgtcgcc aacgccgacg cggcgatgta
     1321 ccaggcaaaa gcgtctggcc gagcgggtgt cagcacgttc gatgagggca tgcgagcaca
     1381 gtcgcgcgac cggctgcaca tggagtcggc tctgcggcgc gcgatcgaca aggcagagtt
     1441 gcgattggtc taccaaccgc aggtcgacct cgcatcgggt cagctcgtcg gcgtcgaggc
     1501 gttgctgcgc tgggattctg aggaactcgg tgccatcgct ccggcagagt tcatcccggt
     1561 cgccgaggaa accgatctga tcatgggcat cggtgagtgg gtgttacagg aggcctgcac
     1621 gcaggcgggc cgatggcagg agagccgcgg ctggtcaccg ctggtgtcgg tcaacgcatc
     1681 ggcgcgtcag ttcggtcggc cctcggtctt ccttgagaac gtgacgagcg cgttaggtgc
     1741 gtcgggcctc gaacccggcc agctgcgcat cgaggtgacg gagtcgacgc tcggcgaccc
     1801 ggcaacatct ggcccgctcg tcgccaggct caaagggctt ggcgtgtcac tgtcgatcga
     1861 cgacttcggc accggctttt cttccctcgc gcgactgcgc agcttccccg tcgacgaact
     1921 gaagatcgac cgctcgttcg tcgagggact cgagacccag gccgacgatc gggccgtcgt
     1981 cgccgccgtc atcggaatgg cgaaagccct ccacctcgac acggtcggcg agggcgtcga
     2041 gaccgtgggc caactcgaca tcttgcgaac ccttggttgc acccgcgtac agggctacta
     2101 cctcggccgg ccgatgcccg cccacgacat caccccgctg ctctcctctc gctgaaaggc
     2161 ggccgcctcg cttatggtgc ggtcgcggtc gcgaacaccc ggcgcagtgc gtcgaaggtg
     2221 tcgagcgacc gtcggccctc ggcttggagg gactgcatcg ctggatcgga atccgccggg
     2281 gcggccaccg ccgcctgcca cacggtgcgc tcgaccgcac gccagtcagc cttgtcgcgc
     2341 acctgcacat cgacgaagcc ggcagccgac aggtcacgat cgagatgaac cgctttgatt
     2401 cgtggtggca cccgattgtc tgaccggtcg ctcgcttccc agcaggtcat cacgagccgc
     2461 ccccctggcc tcagtagccg cctaaactcc accatcgcgg aaagtggcgg ttcggcgaac
     2521 tgaaccgagt caatgcacat cagcccgtca ccgatacctg ccggcaggcc cgtcgcgtcg
     2581 agcgctccaa cgtggaactg ggcgcggcca gtgggcagtt ccaatcgggc agtccgtcga
     2641 cgagcctgtt ccaccgcaac ggcggagaaa tcgatgccga tcagtcgaga tccggtccgc
     2701 cgagcgattt cgattccata gccgccgcgc ccacatgcga cgtcaagcac cagtgcgtcg
     2761 tcgcgtaggt gcagcgctgt tgtgacctcg tcgatggcgt gcgggctcaa gaggcccgtc
     2821 gattccaacc cagacggcaa gctgaggatg cgagcaatga gggcgtcgcg ggtgggcgag
     2881 ctttccatgt ccgcgtacca ctggtcgaag taggcggcgt cgcgtttcac gacggacggc
     2941 ccctcaccca taggggactt tctcctgtct ttgtctactt gccgtggcgg ctgcccacaa
     3001 cgacgactag ctactgacga cgacctcgac gcgctggaac tccttcaggt cggagtatcc
     3061 gcaggtggcc atcgccttgc gcagcgcgcc gaccagattg gtcgtgcccg ccgaatcggt
     3121 cgagggacca tagaggatct cgcgcaaact ccccgcggta cctgcgtggt agcgctggcc
     3181 gcgcgcgagt tcggtgtggc gagcctcggc gccccaatgc cagcccctgc cgggcgcttc
     3241 gctggcgcgc gcgagtggga gcccgatcat caccgcgtca gcaccgcagg cgatcgcctt
     3301 cgcaatatca ccgccggtgc gcatcgcacc gtccgcgatg acgtgcacat agcgaccgcc
     3361 ggactcgtcc atatagtcgc gtcgagccgc cgccgcatca gagacaacgg tcgccatcgg
     3421 gacgccgatc ccgagcacct gtcgcgtcgt gtgcgccgcc ccaccgccga cgcccacgag
     3481 cacgccggcc gcgccggttc gcatgaggtg cagggcggct tggtaggtgt aagacccgcc
     3541 gacgatgacc ggcacgtcga ggtcgtagat gaacttcttc aggttcaacg gttcggcgcg
     3601 gcccgagaca tgctcggccg acacggtagt gcctcggatc acgaaaagat cgacaccagc
     3661 ttcgaggacc gccttgtgat actgcgcggt gcgctgcggt gacaacgctg ccgccgtgac
     3721 gacaccaccg tcacgtatct gcttgatgcg tgccccgatc agctcttcct tgatcggctc
     3781 ggcgtagatc tcacgcatcc gccgcacgac atcgggctcc gccaaagtgg cgatctcgtc
     3841 gagcagcggc actgggtcgt cgtagcgggt ccacaaaccc tcgaggtcga gcacgccgag
     3901 cccgccgagg cgcccgatct cgatcgccgt gtcgggagaa accacgctgt ccatcgcgct
     3961 cgaaacgatc ggcgcctcga atcggtacgc gtcgatctgc cacgcgaccg agacctcttc
     4021 ggggtcgcgg gtgcgtcgcg acggcacgat cgcgacgtcg tcgaacccgt aggcgcgacg
     4081 ggccatcttg cccataccga tctggacctc ggccacccgg aagcccttct gtgcaagcgt
     4141 tcagcgggag tggtagttcg gcgcctcggt cgtcatgtgg acgtcgtggg ggtgactctc
     4201 tttcaacccg gcggacgtga tgcgaatcag ccgcgcctgc tggagttgct cgatggtgtg
     4261 tgcaccgcag taacccatgg ccgaacgcaa accgccgaca agctgatgga cgacggcagc
     4321 gagcggtccg cgataaggga cctggccttc gatgccttcg ggcaccagct tgtcgtcgga
     4381 caacacgtcg tcctggaagt acctgtcctt gctgtacgaa cgcgcttcgg cacgcgattg
     4441 catggcaccg agggatccca tgccgcggta gctcttgtac tgcttgccgt tgatgaagat
     4501 gagctcgccc ggactctctt cgacgccggc gagcaagctg cccagcatca cggcgtcggc
     4561 accgaccgcg atcgccttcg cgatgtcgcc ggagtactgg agaccaccgt cgccgatcac
     4621 gggcacaccg gctgcccggc acgcctgcgc cgcctcatga atcgcggtca cttgcgggac
     4681 gccgacaccc gtgacgaccc gcgtggtgca gatcgaaccc ggcccgacgc cgaccttcac
     4741 gccgtcggcg cccgcgtcga ccaacgcctg ggctccttct cgggtcgcga tgttgccacc
     4801 gatgacgtcg accgtgacgt cgcg
//
LOCUS       DASWVF010000824         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_150708, whole genome shotgun sequence.
ACCESSION   DASWVF010000824 DASWVF010000000
VERSION     DASWVF010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150708"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VGD55_14570"
     CDS             complement(<1..565)
                     /locus_tag="VGD55_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0871619.1"
                     /translation="MTAVLLAEDDPSISEPLARALRREGYEVSVRDEGGEALRLVLAG
                     GIDLVVLDLGLPTMDGLEVCRRLRAEGRSVPVLVLTARVEEVDLVVGLDAGADDYVTK
                     PFRLAELLARVRALLRRGGTEVPDVNGVRVNAESRRAWLGDEELTLTAKEFDLLNTLV
                     LGAGKVITREQLMRDVWDASRGTSSKTL"
     gene            complement(591..2114)
                     /locus_tag="VGD55_14575"
     CDS             complement(591..2114)
                     /locus_tag="VGD55_14575"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY0871620.1"
                     /translation="MSDLTPAELRERIVTGGAAKYHDANAAKGKLFARERVRLLVDNG
                     SFVEDGAFANALAADLPADGVITGTATIDGRPVALLANDSTVKAGSWGARSVEKMLRI
                     AEFAYTVGIPLVYLVDSAGARITDQVAMFPGRRGAGHLFHTQVRASGSIPQVCALFGP
                     SAAGGAYVPAFCDVVIMVEGNASMYLGSDRMVEMVTGEKTTLEAMGGARMHCTESGVG
                     HILVQTEHEALDAIRRYLSFLPSNWRGEPPRVPANDPEPIDLRALVPANERVAFDMLR
                     FVRGLVDADSFFELHALWARELVVGLARLDGRPVGIVANNSLHKGGVLFVDSADKGAH
                     FISLCDAFNVPLVFLADVPGFMVGTAVEKQGIIRHGAKMVSAVSEATVPKFSVIVRKA
                     YGAGLYAMAGPGFDPDATLALPTAKIAVMGADAAVNAVHANRLAAIADDDERAATIAD
                     LRAEYEADIDIVRLAAELVVDDIVQPEELRANLIRRLVVVRTKDRAFSQRRHGITPV"
     gene            2200..2886
                     /locus_tag="VGD55_14580"
     CDS             2200..2886
                     /locus_tag="VGD55_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871621.1"
                     /translation="MAEYRDPPGEAPWAMPIVVRVERGAEPTAAAVAEAAATAVVRLL
                     DDPRSTEGDWHPQVQRWIDGRIRKVARRARGAQWDAASHLPGITVIHAGAEVRAFVPG
                     STDAVPAAIAKLQVAGLDLDPGTPSSDLPGVLAVAVTPLVAMSPLKAAVQVSHAAQLA
                     LTTLAGTPSGARDLESWRATRFGVRLVRPNSDEWARLTTSARIVVRDAGFTEIPPNTQ
                     TAVALWSGAL"
     gene            complement(3003..>3701)
                     /locus_tag="VGD55_14585"
     CDS             complement(3003..>3701)
                     /locus_tag="VGD55_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0871622.1"
                     /translation="ESFPLFGANLDDYDELYEEKLELLLALDRGNPITWSGRFRAPLD
                     DATVLPRPYGDHLRISIATGGNPQSSMRAGVRGLPIVYAVIGGLPERFAPLVNLYRQS
                     GVAAGHDPAALNVTMGAIGLIARDSQDAKDAFYPYWLETMKYGAKARGWAIPSRAEYD
                     QAAHGAQGIFAGSPSEVAERLISVGTLVGADRYAMQMDWAGVPHDRVMTAIELLGTEV
                     LPEVRRALAPVTPG"
BASE COUNT          651 a         1267 c         1208 g          575 t
ORIGIN      
        1 cgagcgtttt cgatgaggtg ccgcggctgg cgtcccacac gtcgcgcatc agctgctcgc
       61 gggtaatcac cttgccggcc cccagaacca atgtgttcag caggtcgaat tcttttgcgg
      121 tgagcgtcag ctcctcgtcg ccaagccagg cccggcgcga ctcagcgttc acccggacgc
      181 cgttcacgtc aggcacctcc gtgccgccgc gccgcaacag cgccctgacc cgcgcgagca
      241 actcggcaag ccgaaacggc ttggtgacgt aatcgtcggc accggcgtcg aggccgacca
      301 ccaagtcgac ctcctcgacg cgagccgtca gcacaaggac ggggaccgag cggccctctg
      361 cccgcagccg ccggcagacc tcgagaccgt ccatcgtggg tagcccgagg tcgagaacca
      421 cgaggtcgat cccgccggcc agcaccaggc gcagggcctc gccgccttcg tcgcgcacgg
      481 agacttcgta accctcgcgg cgcagggcac gagccagcgg ctcggatatc gacggatcat
      541 cctcggcgag caagacggcg gtcatggcga aatggtatgt gccagtgacg ctaaaccgga
      601 gtgatcccgt gacggcgttg tgaaaatgcc cgatccttgg tccggacgac gacgagccgc
      661 cgtatcagat tggcgcgcag ctcctccggc tggacaatgt cgtcgacgac caattcggca
      721 gccaggcgca cgatgtcgat gtccgcctca tactccgcgc ggaggtcggc gatggtagct
      781 gcccgctcgt cgtcgtccgc gatcgcagca agtctgttgg catgaaccgc attgacggcc
      841 gcgtcagcgc ccatcaccgc gatcttcgcg gtcggcaacg cgagcgtcgc gtcggggtcg
      901 aatcccgggc cggccatcgc gtacaggcca gcgccatacg ccttgcgcac gatcacggag
      961 aacttcggaa ccgtcgcctc tgagacggcg ctgaccattt tcgcgccgtg cctgatgatg
     1021 ccctgcttct ccactgccgt gccgaccatg aatccgggaa cgtcggcgag gaagaccagt
     1081 ggcacgttga acgcgtcaca cagcgaaatg aagtgggcac ccttgtcggc ggagtcgacg
     1141 aacagcacgc cgcccttgtg cagggagttg ttggcgacga tgccgaccgg gcggccgtcg
     1201 aggcgagcca gcccgaccac gagttcgcgc gcccacagcg catgcaactc gaagaagctg
     1261 tctgcatcga cgagtccgcg gacgaaccgg agcatgtcga aagccacccg ctcgttggcc
     1321 ggaaccagtg cgcgcaggtc gatcggctcg gggtcattcg caggaacgcg cggtggttca
     1381 ccacgccagt tcgacggcag gaacgacaga taccggcgaa tcgcgtcgag cgcttcgtgc
     1441 tcggtctgca cgaggatgtg cccgaccccc gattccgtgc aatgcatgcg agcgccaccc
     1501 atcgcttcga gggtggtctt ctcgccggtc accatctcga ccatgcggtc ggagccgagg
     1561 tacatcgacg cgttgccctc gaccatgatg accacgtcac aaaatgcagg tacgtaagcg
     1621 ccgcccgctg cactcggacc gaacaacgcg cagacctgag ggatcgagcc actggcccgc
     1681 acctgagtgt gaaacaggtg gccagcacct cggcgtccgg gaaacatcgc gacctgatcg
     1741 gtgatgcgcg cgccggcaga atcgacgagg tagaccagcg gaatccctac tgtgtaagcg
     1801 aattcggcga tgcgcagcat cttctcgacg ctgcgtgcac cccaggagcc cgctttcacg
     1861 gtggagtcgt tggcgagtag agcgaccggc cggccgtcga tcgtggcggt accggtgata
     1921 accccgtcgg caggcaggtc tgccgcgagc gcattcgcga aggcgccgtc ctcgacgaac
     1981 gagccgttgt cgaccagcaa ccgcacccgc tcgcgggcaa acagcttgcc cttcgcggcg
     2041 ttcgcgtcgt gatacttcgc cgcgccgccg gtgacgatgc gctcgcgcaa ctcggccggg
     2101 gtcagatcgc tcattcgccg aatgtaccca cccgggaggc tcggcggcgc cacgatcggc
     2161 gggcgtcaac ctacgaacgg ggtgcgtcaa gatggtcggg tggccgaata tcgcgacccg
     2221 ccgggtgaag cgccgtgggc gatgccgatt gtggtgcggg tcgaaagggg cgccgagccg
     2281 accgccgccg ccgtcgccga ggccgcagcg actgccgtcg tccggctgct cgacgatccg
     2341 cgcagcacgg agggcgactg gcacccgcag gtgcagcgct ggatcgacgg ccggatccgc
     2401 aaggtcgccc gccgggcccg cggcgcgcag tgggacgccg cgtcgcacct gccgggaatc
     2461 acggtcattc atgccggcgc cgaggtacgc gccttcgttc cggggtcaac tgacgcggtg
     2521 cctgctgcta tcgccaagct ccaggtcgcc ggcctcgacc tcgaccccgg caccccgtcg
     2581 tccgatctgc ctggtgttct tgccgtagcg gtcaccccgc tcgtcgcgat gagtcccctc
     2641 aaggcagcgg tgcaggtctc tcatgccgcg caactagcac tcacaacact tgcggggacg
     2701 ccgagcggtg cccgcgacct cgaaagctgg cgggcaacga ggttcggcgt acggctcgtg
     2761 cgaccgaact ccgacgagtg ggcgcgcctg acgacgtcag cgcggattgt ggtgcgtgac
     2821 gccggcttca ccgagatccc gccgaacacg cagaccgccg tcgcgctatg gtcgggagct
     2881 ctatgaacgc acggcccgct caggagaagg ctcggtagca gccgcccgta acgccggatc
     2941 ggtgacagcg gcacctccct atgccgacac cgaaatcgta ggcccgaaag agtccgacct
     3001 tctcacccag gtgtgacagg tgcgagcgcg cgacgaacct ccgggagcac ctcggtgccc
     3061 agtagttcaa tcgctgtcat cacccgatcg tggggcactc ctgcccagtc catctgcatg
     3121 gcgtaacggt cggcgccgac gagggtgccc acggagatga gtcgctccgc aacctcgctc
     3181 gggctccccg cgaagatgcc ctgagcgccg tgcgcagcct ggtcgtattc ggcgcgactc
     3241 ggaatcgccc acccccgggc cttggctccg tacttcatgg tctccagcca atacggatag
     3301 aaggcgtcct tcgcgtcttg ggagtcgcgg gcgatcagcc cgatcgcgcc catcgtgacg
     3361 ttgagtgccg cggggtcgtg gccagctgcg acaccggact gtcggtacag gttcacgaga
     3421 ggagcgaatc gttccgggag accgccgatg acggcgtaca cgatcggcaa tccacgcaca
     3481 cccgcccgca tcgatgactg cgggttgcct cccgtcgcga tcgagatacg caagtggtcg
     3541 ccgtaagggc gcggcagcac cgtcgcgtcg tcgagcggcg cccggaaccg gcccgaccag
     3601 gtgatcgggt taccccggtc gagggcgagg agcagttcga gtttctcctc gtacaactcg
     3661 tcgtagtcgt caaggttcgc accaaacaac ggaaatgact c
//
LOCUS       DASWVF010000825         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_380122, whole genome shotgun sequence.
ACCESSION   DASWVF010000825 DASWVF010000000
VERSION     DASWVF010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380122"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..2323)
                     /locus_tag="VGD55_14590"
     CDS             complement(311..2323)
                     /locus_tag="VGD55_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0871623.1"
                     /translation="MPEKTPLSTRTPAPTKKPAPAKKAVATKRTTPAKKAVPTKEAAR
                     ATTAVAAKKAVPAKKAVPAKKAVPAKKAVPAPNPVLALKPVLAATSALTVTPATRAAS
                     LPPQSANVSAAPVLVMASATKTALATRAPLALTITPARTRATRVTPRTDASRTDASRT
                     DAPPVADARPPASANGNADESVGDRDVGRATGSTVRSGLAWTLAGQWAGYAVQLVATA
                     VLARLVAPKEFGLLSEALTVTAFAAQLQTLGLSQAVVQRAKLTHGQMSNLFWVNAAAG
                     LGLALLVLASAPLVADFYGDHALIGITAALGVTYVIAGLGVQHAALMSRRMKFKAIAV
                     RSLVPRVLAGGIAIAAAVLGAGYWALVIQQIAATFFMVLFVWTAIDWRPSWPARRTGV
                     RPLLRFGAGVSVANILYYFSSNADNILVGRVLGTGPLGLYARAYNLFLVPLRQLHGPL
                     GNVVQPVMSAIVGEPERYRAFYRRMLGAITMVGMPGVVFLAVMSREIIIVVLGQRWVG
                     AAEPFRWLAVAGFLQMISRTFTWLFTTSGRSRAMAIWAGISAPVAVAAFVVGLHWGIT
                     GVAAAYAIAQTGLIVPGIWFAVRGTPVRMSDVADAVWRPAVVAGVVGVAVALVRLALA
                     GSPAVTVLLIGLVVAAVCWACIVSCWPTLRADLLAVKGTLTRRNAR"
     gene            complement(2316..3047)
                     /gene="ribA"
                     /locus_tag="VGD55_14595"
     CDS             complement(2316..3047)
                     /gene="ribA"
                     /locus_tag="VGD55_14595"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HEY0871624.1"
                     /translation="MSVRILEVSKDVPVRNRIGLFTLRAYLIAEGPHIDTHLAMFKAP
                     FTDPVPVRINSACLTGEVFGDRRCDCAWQMWEALRRFEARGQGVMTYHPSHEGRGIGL
                     FQKIRSYELMDEFGITTREAFQQLGEMPDVRTYGAATAILDHLGVRSAQFLSNNPEKI
                     KALEESGIEVVGSERVVMDFDSSLVQYLQSKAVQFGHLIELPTFMLGVPESEDLALWP
                     REVGEHGIALDDVREVADQPTATGA"
     gene            complement(3044..3751)
                     /locus_tag="VGD55_14600"
     CDS             complement(3044..3751)
                     /locus_tag="VGD55_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0871625.1"
                     /translation="MTRALVLGARGGIGSAVCTTLKASKVDEIDAVDIAAVDIADKTD
                     SDHVLDLTDDDTRDAWLKTYLADVGSPDFVVWCAGLYPRRDIREYSTAEVRNIVDVNL
                     IAMLTLLPGLVRASVEAAKPLRIVVVGSQAAVTGGLDVPYAASKAGLVAAVKSIAREY
                     ARHGITANVVSPGPVETAMASVMGDERRAFYESAIPLGRYSRPDEVAAVVGWLLTEAP
                     AAINGTVVDVDGGLVRR"
     gene            complement(3748..>3927)
                     /locus_tag="VGD55_14605"
     CDS             complement(3748..>3927)
                     /locus_tag="VGD55_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871626.1"
                     /translation="DGSSRKREELDLVRMTKVRNYFFEQPWGSSERFQIVDGSESQLE
                     VTAQVLRLLKEFDPR"
BASE COUNT          732 a         1293 c         1267 g          635 t
ORIGIN      
        1 gacgggaacg gatgcggata accaagtcca aacatcggcg ccacaacctc gggcactgaa
       61 ataccgaccc gtccccaggt gcaacacgac ggcttcaccg tcaatgactt ggttgatcac
      121 atctggctca ttgacgcgaa actgctggct ttgcatcgct cagaatcccc tcggtcggtg
      181 gtcatcaaac tgtagagacg aagcgggccg ggcacggccg ttactgcaga tccgcgcaca
      241 cgagagcgca cgaagagagg gcgcgcgcga tgcggaatga cgcgtcggcg tgttgcccgc
      301 ctcgactcgg ttagcgcgca ttgcgccggg tgagggtgcc tttgacggcg agcagatcgg
      361 cgcgaagcgt cggccagcac gaaacgatgc aggcccagca caccgcagcg accaccaaac
      421 cgatgagcag gacagtcacg gcaggcgacc cggcgagtgc taaccgaacg agcgccaccg
      481 cgacgccgac gacacctgcg acgactgccg gacgccacac ggcgtccgcc acatcgctca
      541 tgcgcaccgg cgtgcctcgt acggcgaacc agatgccggg cacgatcaga ccggtctgcg
      601 cgatcgcgta ggcggccgcg acgccggtga ttccccaatg cagaccgacg acgaaggcgg
      661 cgacggcgac cggcgcgctg atgccggccc agatcgccat cgcgcgcgaa cgccctgagg
      721 tcgtgaacag ccaggtgaag gtgcgcgaga tcatctgcag gaagccggcg acggcgagcc
      781 aacggaacgg ttcggccgcg ccgacccaac gctgcccgag gacgacgatg atgatctctc
      841 gcgacatgac cgcgaggaac accacgcctg gcatgccgac catcgtgatg gccccgagca
      901 tccgtcggta gaacgcgcga tacctctcag gttcaccgac gatcgccgac atgacgggct
      961 gcacgacgtt tccgagcgga ccgtggagtt ggcggagcgg cacgaggaac aagttgtagg
     1021 cgcgcgcgta caacccgagc gggccggtgc cgagaacgcg gccgacaagg atgttgtcgg
     1081 cgttgctcga gaagtagtag aggatgttcg cgacgctgac gccggcaccg aaccgcagca
     1141 gcggccgcac tccggtgcga cgcgcgggcc aactcggacg ccaatcaatc gccgtccaca
     1201 cgaaaagcac catgaagaac gtcgccgcga tctgctggat caccaatgcc cagtaccccg
     1261 cgccgagcac cgccgcggcg atcgcgatgc cgcccgcaag cacgcgcggg acgagtgagc
     1321 gtacggctat cgctttgaac ttcatccgac gcgacatcaa cgcggcgtgt tgcacgccaa
     1381 gacctgcaat gacgtaggtc acgcccagtg cggcggtgat accgatgagc gcgtggtcgc
     1441 catagaagtc cgcgacgagc ggtgcggagg cgaggacgag cagcgccagt cccagtccgg
     1501 ccgccgcatt cacccagaac aggttgctca tctggccgtg cgtgagcttc gcgcgttgaa
     1561 cgaccgcctg gctcaatccg agcgtctgca actgtgccgc aaacgccgtg acggtgagtg
     1621 cctcgctcag cagtccgaac tctttcggcg cgaccaaccg agccaacacg gcagtcgcca
     1681 cgagctgcac ggcgtacccg gcccactgac cggcaagggt ccaggcgagt cctgaccgca
     1741 cagtcgaccc cgtcgcgcgc ccgacgtcgc gatcaccaac gctctcgtcg gcatttccgt
     1801 tggcggacgc cggggggcgg gcgtcggcga ccggtggtgc gtcggtacgt gatgcgtcgg
     1861 tacgtgatgc gtcggtacgt ggcgtgaccc gcgttgctcg agtccgtgcc ggcgtgatcg
     1921 tcaaggcgag gggagctcgc gttgccagcg ccgtctttgt cgctgacgcc atgacaagaa
     1981 ccggcgcagc gctaacgttt gcggactgcg ggggtagcga cgcggctctc gtcgccgggg
     2041 taacggtcaa cgccgaggta gcggccagca caggcttaag ggcgagcacc gggtttgggg
     2101 cgggcacagc cttcttcgcg ggcacagcct tcttcgcggg cacagctttc ttcgcgggca
     2161 cagccttctt cgcggccaca gccgtcgtgg cccgcgctgc ttccttggtg ggcacagctt
     2221 tcttcgcggg cgtggtccgc ttcgtggcca cagccttctt ggccggcgct ggcttcttgg
     2281 tgggcgcggg cgtccgagtg ctgagcggcg tcttctcagg caccggtcgc tgtcggttgg
     2341 tcggccacct cgcgcacgtc gtcgagagca ataccgtgtt cgccgacctc gcgcggccac
     2401 aacgcgagat cttctgactc gggcaccccg agcatgaacg taggcaactc gatgaggtgc
     2461 ccgaactgca cagccttgct ctggaggtac tgaacgagcg agctgtcaaa gtccatcacg
     2521 acacgctcgg agccgacgac ctcgatgccg ctctcttcga gcgctttgat cttctccggg
     2581 ttgttgctca ggaactgcgc ggagcgtact ccgaggtgat cgagaatcgc cgttgctgcc
     2641 ccatacgtgc gcacgtcagg catctcgccg agctgctgaa atgcttcgcg tgtggtgatg
     2701 ccgaactcgt ccatcagctc gtacgaacga atcttctgga acaaaccgat gccacgaccc
     2761 tcgtgcgacg ggtggtaggt catcacgccc tgcccgcgcg cctcgaagcg tcgcagcgcc
     2821 tcccacatct gccaggcgca gtcacaccga cggtcgccaa atacttctcc ggtaagacag
     2881 gcggagttga tccgcaccgg caccgggtcg gtgaaggggg ccttgaacat cgccaggtgg
     2941 gtgtcgatgt gcggaccttc ggcaatgaga tacgcgcgca gcgtgaatag accgatgcgg
     3001 ttgcggaccg gcacgtcctt gctgacctcg aggatgcgaa cgctcatcgt ctgaccagcc
     3061 cgccatcgac gtcgaccaca gtgccgttga tggctgccgg cgcttcggtg agcaaccacc
     3121 cgacgacggc cgcaacttcg tcaggccggc tatagcggcc caacgggatc gcactctcgt
     3181 agaaggcccg ccgctcgtcg cccatcaccg acgccatcgc ggtctcgacc gggcccgggc
     3241 tcacaacgtt cgcggtgatg ccatgccgtg cgtattcgcg ggcgatgctc ttgaccgctg
     3301 cgaccaggcc agccttcgag gcggcatacg ggacgtcgag cccacccgtc actgccgcct
     3361 ggctaccgac gacgacgatg cgcagaggct tcgccgcttc gacggaggcg cgaaccaatc
     3421 caggcagcaa tgtgagcatc gcgatcaagt tcacgtcgac gatgttgcgc acttcggcgg
     3481 tggagtactc acggatatcg cggcgagggt acagcccggc gcaccacacg acgaagtctg
     3541 gtgacccaac gtcagcgaga tacgtcttca accacgcgtc gcgtgtgtca tcgtcggtca
     3601 ggtcgagcac atgatcagag tcggtcttat cggcgatgtc gaccgcagct atgtcgactg
     3661 cgtcgatctc gtcgaccttc gacgctttca aagtcgtgca cacagccgag ccgatcccgc
     3721 cgcgcgcgcc taaaaccagt gcgcgcgtca tcgggggtcg aactccttca ggaggcgcaa
     3781 gacttgggcg gtgacctcga gttgtgactc ggaaccatcg acgatctgga agcgctctga
     3841 tgagccccat ggctgttcga agaagtagtt gcgcaccttc gtcatgcgaa caaggtcgag
     3901 ctcttcgcgc tttcgtgacg agccgtc
//
LOCUS       DASWVF010000826         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_150879, whole genome shotgun sequence.
ACCESSION   DASWVF010000826 DASWVF010000000
VERSION     DASWVF010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150879"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VGD55_14610"
     CDS             <1..428
                     /locus_tag="VGD55_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0871627.1"
                     /translation="QKVQALDAGADDYVTKPFGMNELLARLRAATRRATPGVDEPVVA
                     TTEFTIDLGAKRVVLKDGRDVRLTPTEWQLLEVLARHGGRLVSGRQLLQDVWGPAYGT
                     ETNYLRVYMAQLRRKLEPDPSRPRYLITEPGMGYRLVIA"
     gene            452..1222
                     /gene="iolB"
                     /locus_tag="VGD55_14615"
     CDS             452..1222
                     /gene="iolB"
                     /locus_tag="VGD55_14615"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY0871628.1"
                     /translation="MRTVVTPGQDTDLIKLEVADIEAGGSLRYSAEGEALVVILHGVL
                     DIEVDGASLGRAGGRASVFDGGGDAVYLPPGAALMLTETDAAGAAIAVASAPLAEEPA
                     APARVIRPADHRVADVGEGNWARCVRTILGPEHAAGRLLVGETVNPAGNWSSYPPHKH
                     DEHNPPHEVALEEVYLFKIDPPGGFGVQILYDPGPSGERAAVVHDGDVAVIRSGYHPV
                     VGAPGYSLYYLWVMAGQGRQMIPSLDPQHAWVQLEQGR"
     gene            1219..2466
                     /gene="manD"
                     /locus_tag="VGD55_14620"
     CDS             1219..2466
                     /gene="manD"
                     /locus_tag="VGD55_14620"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEY0871629.1"
                     /translation="MSDPGTQAVGSGSRIIDAKVIVTCPGRNFVTLKLTTADGATGVG
                     DATLNGRELAVASYLQDHVCPALIGRDSARIEDTWHYLYKGAYWRRGPVTMTAIAAVD
                     TALWDIKGKLAGLPVYELLGGRARDAVTVYGHANGRDIPEVLDEVAKYLELGYKAIRV
                     QVGVPGLPATYGVSDDKLFYEPAVAGAVPTEATWSTTRYLRFVPGLFKAVRETHGFDF
                     HLLHDVHHRLTPIEAARLGKRVEEFQLYWLEDPTPAESQDAFRLIRQHTTTPIAVGEV
                     FNSIWDCQQLITEQLIDFIRATVVHAGGITHLRRIFNLAELYQVRSGSHGATDLSPVC
                     MAAALHLDLAIPNFGLQEYMRHTAATDEVFPHSYWFADGYLHPGETPGLGVDIDETLA
                     ARYPYRPAPLPVNRLLDGTMHSW"
     gene            2463..3938
                     /locus_tag="VGD55_14625"
     CDS             2463..3938
                     /locus_tag="VGD55_14625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY0871630.1"
                     /translation="MTSRLSIARWDQIPAAAKPEIDPRDLTVGIVHLGVGAFHRAHQA
                     VYTERAMASSGETRWGICGVSQRSRAVIDDLAPQDGLYTVVERGGSSAFRVRAPLREL
                     LFAHDASLLSRLAAPSTHVVSLTVTEKGYRHDPATRRLNTDDAEIQADALGRPPVTVV
                     GQLVRGLTARYRENGAPLTVVCCDNLTDNGGTLRGLVDDFCGLLPPDPRSALASWIAE
                     NVTFPSTVVDRIVPATTAIDRSDVATFLGMDDEGPVVTEPFSQWVLEENFATPRPAWE
                     SAGAVFTADIRPYEALKLRLLNGSHSALAYLGLLAGYQYVAEFLEVDAVAAYVRALMA
                     ETATTMTAPLGFDVEAYEQQLLERFANRGLQYRLAQIATDGSQKLPLRLVATVNDLRA
                     AASEPTLNVLAIAAWMRFVSAQHDDAGAILQIDDPLAQRIAEVVDGATDPARIVDRLL
                     SLSEIFGDLSADKAVRGALVEALSELTRGGALVSLRNATRA"
BASE COUNT          710 a         1362 c         1358 g          760 t
ORIGIN      
        1 tacagaaggt gcaggcgctc gatgccggcg ccgacgacta cgtgaccaag cctttcggca
       61 tgaacgagct tcttgcgcgg ctgcgggcgg cgacccgccg ggccacacct ggcgtcgacg
      121 agccggtcgt tgctacgacg gagttcacga tcgatctcgg cgccaaacgg gtggtgctca
      181 aagacggtcg tgacgtgcgg ctcaccccca ccgagtggca actgctcgag gtgttggcga
      241 ggcacggtgg ccggctggtc agcggtcggc aactgctcca ggatgtgtgg gggccggcgt
      301 acggcaccga gaccaactac ctgcgcgtgt atatggcgca gttgcgccgc aagctcgaac
      361 ctgatccgtc tcgtccgcgc tatctcatca ccgagccggg catgggctac cggctggtca
      421 tcgcctagtc gcaggaagga agcgtgtcgg cttgcgcacc gttgtcacac ctggtcagga
      481 caccgatctc atcaagctcg aggtcgccga catcgaggct ggcggctccc ttcggtattc
      541 ggccgagggt gaggcactgg tggtgatcct gcacggggtt ctcgacatcg aggtcgacgg
      601 cgcgtcactt ggtcgcgcag gcgggagagc cagtgtcttc gacggtggcg gcgatgcggt
      661 ctacctgccg cccggtgctg cactgatgtt gaccgagacg gacgccgcgg gcgcggccat
      721 cgccgtcgct tcagctcctt tggctgagga gccggccgcc ccggcgcggg tgatccggcc
      781 cgctgaccat cgggtcgccg acgtcggcga aggaaattgg gcacgttgcg tgcgcacgat
      841 tctcgggcca gaacatgcag ctggtcggct ccttgtcggc gagacggtga acccggcagg
      901 caactggtcg tcgtatccgc cgcacaagca cgacgagcac aacccaccgc acgaggtggc
      961 actcgaggag gtctatctct tcaaaatcga tccacctgga ggtttcggcg tgcagatcct
     1021 ctatgacccc ggacccagcg gcgagcgggc cgcggtcgtg cacgacggcg atgtcgccgt
     1081 catcaggtcg ggctatcatc cggtcgtggg cgcgcccgga tattcgctgt actacctgtg
     1141 ggttatggca gggcaagggc gtcagatgat cccctcgctc gatccgcagc acgcatgggt
     1201 gcaactcgag cagggccgat gagcgatccc ggaacgcaag ccgtcggtag cgggtcgcgc
     1261 atcatcgatg ccaaagtcat cgtcacctgc ccaggtcgca acttcgtcac cttgaagctg
     1321 acgactgcgg acggcgcgac cggcgtcggt gacgcaaccc tgaacggcag ggaacttgcc
     1381 gtcgccagct acctgcaaga ccacgtgtgc ccggccctga tcgggcgcga ctcggcacgc
     1441 atcgaagaca cctggcacta cctctacaaa ggcgcctact ggcgacgcgg gccggtcacg
     1501 atgacggcga tcgctgcggt cgacacggca ctgtgggaca tcaaaggcaa actcgcgggg
     1561 ctgcctgtct acgagctgct cggcggccgg gcgcgtgacg ctgtcacggt gtacggccac
     1621 gccaacggcc gcgacatccc cgaggtgctc gatgaagtcg ccaagtacct tgagttgggt
     1681 tacaaggcga tacgggtgca agtcggcgtc cccggtctgc ctgctacgta tggggttagt
     1741 gacgacaagc tgttctacga acccgcggtc gccggagctg tgcctaccga agccacctgg
     1801 tcgaccacgc ggtacctgcg attcgtgccc ggcctgttca aggcggttcg cgagacgcac
     1861 ggcttcgact tccatctgct gcacgacgtg catcaccggc tcaccccgat cgaagccgct
     1921 cgcctgggca agcgggtcga ggagtttcag ctgtactggc tggaagaccc gacgcccgca
     1981 gagtcgcagg acgcgttccg gcttatcagg caacacacga ccacgccgat cgcggtcggc
     2041 gaagtcttca actccatctg ggattgccag cagctgatta ccgagcagct gattgacttc
     2101 atcagggcga ccgtcgttca cgccggcggc attacgcacc tgcgccgcat cttcaacctg
     2161 gctgagcttt atcaggtacg cagcgggtcg cacggggcca ctgatctgtc gcctgtctgc
     2221 atggcggccg cgttgcacct cgacctcgcg atcccgaact tcggcctcca ggaatacatg
     2281 cgacacactg cggcgaccga cgaggtgttc ccgcactcgt actggttcgc cgacggctac
     2341 ctgcatccgg gcgagacgcc tggcctcggc gtcgatatcg acgaaacgct cgccgcgaga
     2401 tatccctacc ggccggcacc cttgccggtg aatcggctac tcgacgggac gatgcacagc
     2461 tggtgacatc acggttgagc attgcccggt gggaccagat cccggctgcg gcgaaaccgg
     2521 agatcgatcc gagagacctg accgttggca tcgtgcatct cggcgtcggc gcgttccatc
     2581 gggcgcatca ggccgtgtac accgaacgcg cgatggcctc ttccggtgaa acgcgctggg
     2641 gcatctgcgg agtttctcaa cgaagtcgtg cggtcatcga cgacctggcg ccgcaggacg
     2701 gcctttacac ggtcgtcgag cggggaggtt cgtcggcgtt tcgggtgcgt gcgccgttgc
     2761 gcgaattgct gttcgcacac gacgcgtcac tgctttcgcg gttggccgcg ccctcgacgc
     2821 atgtcgtctc gctgactgtc acggaaaagg gttaccggca cgatcccgca acccgccggc
     2881 tcaacaccga cgacgctgag attcaggccg atgcattggg tcggccaccg gtcacggtgg
     2941 tcggacagct tgttcgaggt ctcacggctc gatatcgcga gaacggcgcg ccgctcaccg
     3001 tcgtctgctg cgacaacctg accgacaacg gcggcacgtt gcgaggtctc gttgacgact
     3061 tctgcgggct gttgccaccc gacccgcggt cggcactggc gtcgtggatc gcggagaacg
     3121 tcaccttccc ttcgacggtt gtcgatcgga tcgtgccggc caccacggcg atcgaccgca
     3181 gcgacgtcgc aacgtttctc ggcatggacg acgaggggcc ggtcgtcacc gagccgttct
     3241 cgcaatgggt actcgaggag aacttcgcga ccccgcgccc agcttgggag agcgccggcg
     3301 ctgtcttcac agctgacatc aggccctacg aggcgctgaa gttgcgcttg ctcaacggct
     3361 cgcactctgc gctcgcctac cttggcctgc tggcgggcta tcagtacgtt gccgagtttc
     3421 tcgaggtcga tgcggtcgcg gcttacgttc gcgctttgat ggccgaaacg gctaccacca
     3481 tgaccgctcc tctcggtttc gacgtcgaag cctatgaaca gcaactgctc gaacgcttcg
     3541 ccaatcgcgg tcttcagtac cggctggcgc agatcgcaac cgacgggagc cagaagcttc
     3601 cgcttcggct tgtcgcgacc gtcaacgacc tgcgagcggc cgcaagtgag ccgaccctta
     3661 acgtgctcgc gatcgccgcg tggatgcggt tcgtcagtgc gcagcacgac gacgccggcg
     3721 caatcttgca gatcgatgac ccactggccc agcgcatcgc cgaggtcgtt gatggcgcaa
     3781 ccgatccggc tcgcatcgtc gacaggctgc tgagccttag cgagatattt ggcgatctga
     3841 gtgccgacaa ggcagtgcgc ggcgccctcg tcgaggcact ttcggaactg acgcgcggtg
     3901 gcgcgctggt gtcgctgcgt aacgccacca gggcctgaac cgttccatca gtgaaggcgt
     3961 tagacctttg gcgccctctt gcctgctcag aaagtatgac tggcttcagc ttttgcacag
     4021 gttcggccat gacgataggt gtagcgagcc cacgtgattc cggaggtgtg atgacgagcg
     4081 tgctgcccga ccggggcatc cgccgcagca cgtcggcgtc caccatgagc tctggcatcc
     4141 ggcccgacgc actccttgcg gcactgacgc tcggtcgcgt gctcgtcatc
//
LOCUS       DASWVF010000827         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_406019, whole genome shotgun sequence.
ACCESSION   DASWVF010000827 DASWVF010000000
VERSION     DASWVF010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406019"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VGD55_14630"
     CDS             complement(<1..590)
                     /locus_tag="VGD55_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY0871631.1"
                     /translation="MAEMLKGGVIMDVVTADQAKIAEDAGAVAVMALERVPADIRAQG
                     GVARMSDPDLIDAIIAAVSIPVMAKARIGHFVEAQILQSLGVDYIDESEVLTPADYSH
                     HIDKWAFTVPFVCGSTNLGEALRRITEGAAMIRSKGEAGTGDVSNATGHMRQIRDEIR
                     RLSSLPTDELFVAAKELQAPYELVREVAKAGKLPVVL"
     gene            complement(722..1309)
                     /locus_tag="VGD55_14635"
     CDS             complement(722..1309)
                     /locus_tag="VGD55_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871632.1"
                     /translation="MPWWFWLIVAFVLLAGYVTATSNRLDRLHARLDATRAALDAQLL
                     RRAAVTLEIAASGMLDPATSVLLASAGHEARAATGEDADREVVESRLTVALRAAFADP
                     DEAQTLCVEPTGVGLLAELEAATTKVLLARRFSNDAVTATLAIRRHRLVRWLRLAGRV
                     SAPTFFEMDDEPPAYLAVAEPPLNGEAGEGGPDVD"
     gene            complement(1312..2463)
                     /locus_tag="VGD55_14640"
     CDS             complement(1312..2463)
                     /locus_tag="VGD55_14640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0871633.1"
                     /translation="MKVGLVCPYTWDVPGGVQAHVRDLAIALIGLGHDVSVITPADDD
                     SALPPYIVSTGRAVPVPYNGAVARMAFGPLSARRVRRWLRDGEFDVVHVHEPTTPSLS
                     LLATWSFDGPIVATFHTSVQRARTLAAAYPILQTALEKISARIAVSQAARRTLVEHLG
                     GDAVMIPNGVSMASFADVEPFPGWPGEGGALGFLGRFDEARKGLPVLLAAFEKLAETR
                     PGLRLLIAGPGDPDEVRSALPAEVADRVELLGLVSDADKARMLASVDIYVAPNIGGES
                     FGIVLLEAMAVGTPVLASDLDAFRAVLDAGRSGQLFATGDADDLAAKAGELLDDPEAR
                     AALVALGRATARSYDWSTVARDIERVYETVVPGVPDPASPAARRPTGRR"
     gene            complement(2460..>3288)
                     /locus_tag="VGD55_14645"
     CDS             complement(2460..>3288)
                     /locus_tag="VGD55_14645"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEY0871634.1"
                     /translation="SHGPSVRQLERNLRRVVGPEMPDAELRALAREAMRSYGRYWLEF
                     FRLPVLGRERILGRMLFTGEELLVEALARGRGAVLALPHSGNWDHAGAWLVLRGHSFT
                     TVAERLRPEALFDRFVAVREKVGMEVLPLTGGGRTTFAVLLQRLRAGKTLCLVADREF
                     GDGGIEVSFFGEAAEMPAGPASLALATGAALLPACLWYSDEGGPGGSWCGWIRDEIKP
                     PAEGDKRAKVAAMTQALADRFAEGIAEHPQDWHMLQPLWLADVQLTSPQPAQVEGAE"
BASE COUNT          544 a         1168 c         1076 g          500 t
ORIGIN      
        1 agcaccaccg gcagtttgcc ggccttggcg acctcgcgga caagctcgta gggtgcctgc
       61 aattccttgg ctgcaacgaa aagctcgtcg gttggcagcg acgacagccg cctgatctcg
      121 tcgcggatct gccgcatgtg gccggtcgcg ttcgacacgt cgccggtgcc cgcctcgccc
      181 ttcgaccgga tcatcgccgc accctcggtg atacgccgta gggcctcgcc gagattggtc
      241 gaaccacaca cgaacggaac cgtgaacgcc cacttgtcga tgtgatggct gtagtcggcc
      301 ggcgtcagca cttcggactc gtcgatgtag tcgacgccga gcgactggag gatctgcgcc
      361 tcgacgaaat gaccaatgcg cgcctttgcc atgactggga tcgagaccgc agcaatgatc
      421 gcgtcgatga gatcggggtc gctcatgcgc gcgacgccgc cctgcgccct gatgtcggcc
      481 ggcacccgct cgagtgccat gactgcaacc gcgccagcgt cttcagcgat cttcgcctgg
      541 tcggcagtga cgacgtccat gatcacgccg cccttgagca tctcggccat gccacgcttc
      601 acgcggacag aaccagcaac agctgccgcg ggcgccgatg ggtgcgcgtt ctcgcttgac
      661 tctgactcgc ggggcaacgg aaaacctctc taccttgagt tgcggacgca ggaatctgcg
      721 ctcagtctac gtccggaccg ccctcgcccg cctcgccgtt tagcgggggc tcggcaactg
      781 cgaggtacgc cggcggttcg tcgtccatct cgaaaaacgt cggcgccgac acccgcccgg
      841 ccagccgcag ccagcgcact agccggtggc gccggatcgc caacgtcgca gtgactgcgt
      901 cgttggagaa ccgcctggcc agcagcactt tcgtggttgc cgcctcgagt tcggccagca
      961 gccccacccc ggtcggctcg acacacagcg tctgcgcctc gtcgggatcg gcgaacgctg
     1021 cccgcagcgc gactgtcagg cggctctcga cgacctcgcg atcggcgtcc tcacccgttg
     1081 ctgcgcgggc ctcgtgtccg gcactggcta gcaacacgct ggtcgcgggg tcgagcatgc
     1141 ccgacgcggc gatctccaac gtgacggcgg cccggcgcag cagctgcgcg tcgagcgcgg
     1201 ccctggtggc gtcgaggcgc gcgtgcaagc ggtcgagccg gttgctcgtc gccgtcacgt
     1261 agcccgcgag gagcacgaag gccacgatga gccagaacca ccacggcacg cttatcgcct
     1321 cccggtcggg cggcgcgctg cggggctcgc ggggtctggc acacctggca cgaccgtctc
     1381 gtacacccgc tcgatgtcac gcgccacggt cgaccagtcg tagctgcgcg cagtcgcccg
     1441 ccctaacgcc accagtgcgg cgcgcgcctc ggggtcgtcg agcagctcac ccgccttcgc
     1501 cgcgaggtcg tctgcatcgc cggtggcgaa caactggccg gatcggccgg cgtccaacac
     1561 cgctcgaaat gcgtcgaggt cgctcgcgag cacgggcgta ccgaccgcca tggcttcgag
     1621 caaaacgatg ccgaagcttt cgccgccgat attcggggca acgtagatgt cgaccgatgc
     1681 gagcatgcgt gctttgtcgg cgtcactcac gaggcctagc aactcgacac ggtcagcgac
     1741 ttcggcaggc agggcgctgc gcacctcgtc tgggtcgcct ggtccggcga tcagcaatcg
     1801 aagaccgggt cgtgtttcag ctagcttttc gaacgccgcg agcaacaccg gcagcccctt
     1861 gcgcgcctcg tcgaagcgac cgaggaaccc gagcgcgcca ccctcgcccg gccatccggg
     1921 aaagggttcg acatcggcga acgacgccat cgacacgcca ttgggaatca tgacggcgtc
     1981 accgccgagg tgctcgacca gcgtgcggcg ggcggcttgt gacactgcga tgcgcgcgct
     2041 gattttttcc agcgccgtct gcaaaatcgg gtaggcggcg gcgagtgtgc gcgctcgctg
     2101 caccgacgtg tggaacgtgg ccacgatcgg gccgtcgaac gaccaggtcg cgagcagcga
     2161 caagctcggt gtcgtcggct cgtgcacatg cacgacgtcg aactcgccgt cgcgaagcca
     2221 ccgccgtaca cgtctcgctg acagcgggcc gaatgccatg cgcgcgaccg cgccgttgta
     2281 agggactggg acggcgcgcc cggtcgagac gatgtacggg ggcaacgcag agtcgtcgtc
     2341 ggcgggggtg atgaccgaca cgtcgtgacc caagccgatg agagcgatcg ccagatcgcg
     2401 cacgtgggcc tgcactcccc cagggacgtc ccaggtgtat gggcagacca ggccgacctt
     2461 cactcggcac cctcgacctg ggccggctgc ggagaggtca gctgcacgtc ggccagccac
     2521 agcggttgga gcatgtgcca atcctgcggg tgctcggcga ttccctcggc gaaccgatcg
     2581 gccagagctt gcgtcatcgc cgcgaccttg gcccgcttgt caccttcggc cggcggcttg
     2641 atctcgtcgc ggatccagcc gcaccagctg ccgccggggc cgccttcgtc gctgtaccaa
     2701 agacacgcgg gcagcaaagc ggcgccggtc gccagcgcca gtgacgccgg gccggcaggc
     2761 atctcggcgg cctccccgaa gaaggagacc tcgatgccgc cgtcgccgaa ctcacggtcg
     2821 gcgaccaggc atagcgtctt acctgcgcgc agccgctgga ggagcacggc gaacgtcgtg
     2881 cgaccaccgc ccgtcaacgg cagcacctcc atgcccacct tttcgcgcac tgcgacgaac
     2941 cgatcgaaca gcgcctccgg gcgcaacctc tcggcaacgg tagtgaacga gtgcccgcgc
     3001 aagaccagcc atgcgccggc gtggtcccag ttccccgagt gcggcagcgc cagtaccgct
     3061 ccgcggccac gggcaagcgc ctcgacgagc aattcctccc cggtgaacag catgcggccg
     3121 agaatgcgtt cacgaccgag gaccgggagc cggaagaact cgagccagta ccggccgtac
     3181 gaacgcatcg cctcgcgtgc cagcgcacgc aactcggcgt ccggcatctc gggacccacg
     3241 acccgtcgca gattgcgttc gagttgccgc acgctcgggc cgtggctg
//
LOCUS       DASWVF010000828         5345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_176068, whole genome shotgun sequence.
ACCESSION   DASWVF010000828 DASWVF010000000
VERSION     DASWVF010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176068"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGD55_14650"
     CDS             complement(<1..1216)
                     /locus_tag="VGD55_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0871635.1"
                     /translation="MSWSGQSWGFEQPRFVADLTGDGAADVVGFGLEGVWVSLNNGNG
                     TFQPPNMVLSAFGLRTGWMVERHPRFLADLTGDHRADIIGFGADGVWTAANNGDGTFQ
                     PSKFVVADLGYSQGWRVDQHPRFMADVTGDGKADIVGFGADGVWTAVSNGDGTFQSPK
                     LVLADLGYNQGWRVNEHPRMVADVTGDGKADLVGFGADGVWVAVSNGDGTFQPAKLVL
                     AGFNPTQGWQVAKDPRMMADLNGDHRLDIVGFGDAGVWSAKSNGDGTFAAATFIVAAM
                     GYDTGWRVENHPRFAADVTGDGKADIVGFGDDGVWVSVTGGSGANLALTGFCYDQGWR
                     VHDHPRFLADLNSDHKADIIGFGEAGVWVALSNGDGTFQPASFVLADFGQHAGPVVQK
                     ITIDFHTHDDNLN"
     gene            1379..2185
                     /locus_tag="VGD55_14655"
     CDS             1379..2185
                     /locus_tag="VGD55_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0871636.1"
                     /translation="MPTQIPRAWLVRANNPSPMTLEGTNTWVLVEPGELDAIVVDPGP
                     DDEVHRNAVMAAVAHAGASRVALVLLTHGHPDHAEGAPAFARMTDAPVRAAAPELCSP
                     DQRPLADGEHIAQLEVMATPGHTADSVCFVIGADADGHASGAALLTGDTILGRGSTVV
                     ARPDGRLADYLASLRKLRALVDERQLLAVLPGHGPTLDDPAATIDSYLRHREERLEQV
                     HSAAVRAQTVDEVLHAVYSDVDPALMFAARWTLLAQLDYLRESGISVPAD"
     gene            complement(2166..2840)
                     /locus_tag="VGD55_14660"
     CDS             complement(2166..2840)
                     /locus_tag="VGD55_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0871637.1"
                     /translation="MDDVLGKLPLFAALDDDAGVALRVAMRHEQFSRGDVIFEEGDEG
                     DKLFAVIDGKVKLARTAPDGRENLQAVLGPGEMFGELSLFDPKPRTAGAVALTDTVLA
                     SLAHEELRPWITGRPDVAVQLLQALAQRLRRTNDVLADLVFSDVPSRVAKALLGLAER
                     FGQPTSEGIHVAHDLTQEELAQLVGASRETVNKALADFSARGWVRIESRAVVIRDIEG
                     LSRRAR"
     gene            complement(2887..3438)
                     /locus_tag="VGD55_14665"
     CDS             complement(2887..3438)
                     /locus_tag="VGD55_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871638.1"
                     /translation="MKAGSENVSRSGPRRTPERSSDYDHLSIGDLRTYRTALAEEENR
                     VSYWRRIIQARLDVVRAGEAGGARVENLRNVLAEARVETGRRALMTLGAPDDIPPLPD
                     LATLWEREPVADDPDHNENLTRELAAAEVQLSAYRAALHRRISASTDELIARYREDPG
                     LCMLALPADQKEAASRRALADRR"
     gene            3693..4358
                     /gene="nth"
                     /locus_tag="VGD55_14670"
     CDS             3693..4358
                     /gene="nth"
                     /locus_tag="VGD55_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0871639.1"
                     /translation="MYKVLTEVYPDAHCELDFTSPLQLLVATILSAQCTDKRVNMVTP
                     ALFAKYPAAADYAAADRADIEKLIVSTGFFRAKTTSIIGMAQGLCDRFGGEVPPRLKD
                     LVTLPGVGRKTANVVLGNAFGIPGITVDTHMLRLTNRFGWTTSADPVKVEADIAALIP
                     RKEWTLLSQRVIWHGRRVCHARKPACGACALAPWCPSFGLGPTDEASALKLVKTGPVD
                     TLL"
     gene            4355..4978
                     /locus_tag="VGD55_14675"
     CDS             4355..4978
                     /locus_tag="VGD55_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0871640.1"
                     /translation="MRSVRTRAGLTAAGIVAVLIAALLINRASAKHADSSPLRASLIA
                     AAKLPDCPVTTGSGQVADGLPSFTFPCLATGPEVDLAKLRGPLVVNVWAGPCTECRVE
                     APQLRAFAAAATGKVAVLGIVDGAYNGTETWDDALDASRGLGIAYPSVWDANGKFVNF
                     LRVPGIPVSLFVRPDGTVAHLKLGIVQPGELQQLTKQYLGITVAVTP"
     gene            4975..>5345
                     /locus_tag="VGD55_14680"
     CDS             4975..>5345
                     /locus_tag="VGD55_14680"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY0871641.1"
                     /translation="MTTQPPAWLATLIAAAGSLEVSDLSRLQPPEDGGRESAVLVLFG
                     EGRDGPDVLLIERSGHSRQHAGQAAFPGGAVEPDDDGPASTALREAAEETGLDPTGVE
                     VLEVLPALWLPPSGFLVVPVLA"
BASE COUNT          848 a         1840 c         1752 g          905 t
ORIGIN      
        1 cgttcaagtt gtcgtcgtgg gtgtggaagt cgatggtgat cttctgcacg accggcccgg
       61 cgtgctggcc gaagtcggcg agcacgaaac tcgcgggctg gaaggtgccg tcaccgttac
      121 tgagtgcgac ccacacgccc gcttcgccga aaccgatgat gtcggctttg tggtcgctat
      181 tgagatcagc aaggaagcgg gggtggtcat gaactcgcca accctggtca tagcagaacc
      241 cggtcagcgc cagattcgcg ccacttcctc cggtgaccga tacccacact ccgtcgtcgc
      301 cgaagccgac gatgtctgcc ttgccatcgc cagtgacgtc ggccgcgaag cggggatggt
      361 tttcgacgcg ccaaccggtg tcgtaaccca tcgccgcgac gatgaaggtg gccgcagcga
      421 aagtgccgtc gccattactc ttcgcagacc aaactcccgc gtcaccgaac ccgacaatgt
      481 cgaggcgatg atcgccgttc aggtcggcca tcatccgagg gtctttcgcg acctgccagc
      541 cctgcgtggg attgaatccc gcgagcacga gtttggccgg ctggaaggtg ccgtcgccat
      601 tgctcactgc gacccacacg ccgtcggccc cgaatccgac gagatcggcc ttcccgtcgc
      661 cggtcacgtc ggcgaccatg cgcgggtgtt cgttgacgcg ccacccctgg ttgtagccca
      721 gatcggcaag aacgagcttc ggcgactgga atgtgccgtc gccattgctc accgcggtcc
      781 agacgccgtc ggctccaaat ccgacgatgt cggccttgcc gtcaccggtt acatcggcca
      841 taaagcgcgg gtgctggtcg acgcgccagc cctggctgta cccgaggtcg gctacgacga
      901 atttcgacgg ctggaacgtt ccatcgccgt tgttcgcagc ggtccacacg ccgtcagcgc
      961 caaaaccgat gatgtcggca cggtggtcgc cggtcagatc cgccaagaaa cgcggatgcc
     1021 tctccaccat ccatccggtc cgcaagccga atgcggacag gaccatgttc ggaggctgga
     1081 acgtgccgtt cccgttgttg agcgagaccc aaacgccttc gaggccgaac ccgacgacgt
     1141 cagccgcgcc atcgcccgtg agatcagcga cgaatcgggg ttgctcaaaa ccccagcttt
     1201 gaccactcca actcatgttc cacctcggtt cggcgagggc tagaccggtc gttcaacgta
     1261 gcctcgattg cggtcacatg tcgacccatg tcgcatttca aaccgcggat tgcccctagg
     1321 ggaggcggta cctggtctgc ggcgacacgt ctgcagcctg gcacctacgc tcacccccat
     1381 gcccacccaa attccgcggg cctggctcgt gcgtgcgaac aatccgtcgc cgatgacgct
     1441 cgagggcacc aacacctggg ttctcgtcga gcccggcgaa ctcgatgcga tcgtcgtaga
     1501 tcccgggccg gacgacgagg tgcaccgcaa cgccgtcatg gcggcggtcg cccacgccgg
     1561 ggcaagccgg gtcgctctcg tgctgttgac ccacggccac cccgaccacg ccgaaggcgc
     1621 gcccgccttc gcccgcatga cagacgcgcc cgtgcgcgcg gccgcgcccg aactgtgttc
     1681 gcccgaccag cgaccgctcg ctgatggcga acacatcgct cagctcgagg tcatggcaac
     1741 gcccggtcac acggccgact cggtgtgctt cgtgatcggc gccgacgctg acggccacgc
     1801 cagcggcgcc gcattactca ccggcgacac cattcttggt cgcggcagca cggtcgtcgc
     1861 ccggcccgac ggccggctcg ccgactatct ggcatcgctg cgcaagctgc gcgcgcttgt
     1921 cgacgaacgc cagctcctcg ccgtcctgcc tgggcatggt cctacgctcg atgaccctgc
     1981 ggcgacgatc gactcctacc tgcgccaccg cgaagaacgg ctcgaacagg tgcactcagc
     2041 ggctgtgcgc gctcagaccg tcgacgaggt tttgcatgcg gtgtatagcg acgtcgatcc
     2101 tgcgctgatg tttgcggcgc gttggacgtt gctcgcgcag ctcgattacc tgcgtgagtc
     2161 gggaattagc gtgcccgccg actaagaccc tcgatgtcgc ggatcaccac ggcacgcgac
     2221 tcgatacgca cccacccgcg cgcactgaag tcggcgagcg ccttgttgac ggtctcgcgc
     2281 gatgcgccga cgagctgcgc gagctcttcc tgcgtgaggt cgtgcgcgac gtgaatgcct
     2341 tcgctcgtcg gctgtccgaa ccgctcggcg agcccgagca gcgccttcgc gacgcgagac
     2401 ggcacgtcac tgaaaacaag gtcggccaac acatcgttcg tgcggcgaag tcgttgggca
     2461 agtgcttgaa gaagttggac ggcgacatca gggcgcccgg tgatccaggg ccgcaattct
     2521 tcgtgggcga gggacgcgag cacggtgtcg gtcagtgcga cggctccggc ggttcgcggc
     2581 ttgggatcga agagcgagag ctcgccgaac atctcacccg gaccgagcac tgcttgcagg
     2641 ttctcgcgac cgtcgggcgc tgtgcgggcg agcttgacct tgccgtcgat caccgcgaac
     2701 agcttgtcgc cctcgtcgcc ctcttcgaag atgacgtcgc cccgggagaa ctgctcgtgc
     2761 cgcatggcga cgcgcagggc cacgcccgcg tcgtcgtcga gcgccgcgaa gagcggcagc
     2821 ttgccaagca cgtcatccac gtgcgtgatt ctcacacgta accgcgaaac tggcgcgggt
     2881 gagccgtcag cggcgatcgg cgagggcgcg cctgctggcg gcctcttttt ggtcggccgg
     2941 tagcgcaagc atgcacaggc ctgggtcctc gcggtaacgc gcgatgagtt cgtcggtcga
     3001 tgcgctgatg cggcgatgca gcgcggcgcg gtacgccgac aactgcacct cggccgccgc
     3061 cagctcgcgg gtcaggttct cgttgtggtc aggatcgtcg gctaccggtt cgcgttccca
     3121 cagcgtcgcc aggtcgggca gcggcgggat gtcgtcgggg gcacccaggg tcatcagcgc
     3181 ccggcgtccg gtctcgactc gagcctcggc gaggacgttg cgcaggttct cgacccgcgc
     3241 gccaccggct tcaccagcgc ggacgacgtc gagccgggct tggatgatcc gccgccagta
     3301 cgagacgcgg ttctcttcct cggcgagcgc ggtgcggtag gtgcgcaggt cgccgatcga
     3361 gagatggtcg tagtcggagc tgcgttctgg cgtgcggcgg ggccccgagc ggctcacgtt
     3421 ctcgctgccg gccttcacaa cgaccccgtc tcgttaggca cgctccggac acctcacctg
     3481 atccggctac tagaggtgcg catcggcggg tctggcgcga gccttgaggt accggacgca
     3541 accgtcaccc gcccgggggg ctgcgtcacc cggacaggtc agtcggtgcc gggccgtcgg
     3601 cggccccgaa tagagtcgtc ggcgtggcgg gacgggcagt agtagctgca gcagacgaat
     3661 cgcagacctc gcttgtccgg cgggcccgca agatgtacaa ggtgctgacc gaggtctatc
     3721 ccgacgccca ctgcgagctc gacttcacca gcccgctcca gctgctggtg gccacgatcc
     3781 tgtcggccca atgcaccgac aagcgcgtca acatggtcac ccccgcgctc ttcgcgaagt
     3841 acccggcagc cgccgattac gccgcggcgg atcgggccga catcgagaag ctcatcgtgt
     3901 cgacaggctt ctttcgggcc aagacgacct cgatcatcgg catggcccaa ggcctgtgcg
     3961 atcgcttcgg cggtgaggtg ccgccccgcc tgaaggatct ggtcacgctt ccgggggtcg
     4021 gtcgcaagac ggccaacgtg gtgctcggca acgcgttcgg catccccggc atcacggtcg
     4081 acacgcacat gctgcgcctc accaatcgtt tcggctggac cacctccgct gacccggtga
     4141 aggtcgaagc cgacatcgcg gcgctgatcc cacgcaagga gtggacgctg ctttcccagc
     4201 gcgtcatctg gcacggacgc cgggtgtgcc acgcccgcaa gcccgcgtgc ggtgcgtgcg
     4261 cgctggcgcc gtggtgcccg tcgttcggcc tcgggccgac cgacgaggcc agcgcgctca
     4321 agctggtgaa gaccggcccg gtggatacct tgctgtgagg tccgttcgaa cgcgggccgg
     4381 gctgacggcg gccggcatcg tcgccgtgct gatcgcggct ctgctgatca accgcgcgag
     4441 cgccaagcac gccgactcaa gtccgttgcg tgcgtcgctg atcgccgcgg caaagttgcc
     4501 cgactgcccg gtgaccacag ggtccgggca ggtcgccgac gggctgccct cgttcacgtt
     4561 cccgtgtttg gcgaccggcc cggaagtcga cctcgccaaa ctccgtggtc cgctcgtggt
     4621 caacgtttgg gccgggccgt gcaccgaatg ccgcgtcgag gcgccgcagt tgcgtgcttt
     4681 cgcggccgca gcgaccggca aggtcgccgt cctcggcatc gtcgacggcg cctacaacgg
     4741 caccgagaca tgggacgacg ccctcgacgc ctcgcgcgga ctcggcatcg cctacccctc
     4801 ggtctgggac gcgaacggca agttcgtgaa cttcttgcgg gtgcccggga tcccggtctc
     4861 gctgttcgtc cgacccgacg gcactgttgc ccatctcaag ctcggcatcg tgcagcctgg
     4921 cgaactccag cagctcacca agcaatacct cggcattacc gttgcggtga cgccatgacg
     4981 acgcagccgc cggcgtggct cgcgacgctc atcgcggccg cgggcagcct cgaagtgagc
     5041 gacttgagcc ggctccagcc acccgaagac ggcgggcgtg agagcgcagt gctcgtcctg
     5101 ttcggcgaag gtcgcgacgg gcccgacgtg ctgctgatcg aacgcagtgg gcactcccgt
     5161 cagcacgcgg gccaggccgc gtttcctggc ggtgcagtcg agccagacga cgatgggccc
     5221 gcgtcgacag cgctgcgcga ggcggctgag gagaccggcc ttgacccgac aggtgtcgaa
     5281 gtactcgaag tgttgccagc tctgtggctg ccgccgtcgg gctttctcgt cgtccctgtt
     5341 cttgc
//
LOCUS       DASWVF010000829         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_457262, whole genome shotgun sequence.
ACCESSION   DASWVF010000829 DASWVF010000000
VERSION     DASWVF010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457262"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGD55_14685"
     CDS             complement(<1..328)
                     /locus_tag="VGD55_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871642.1"
                     /translation="MSGGALFLAVFLACTVEAVEATTIVLAAGTARDWRSALYGLFAA
                     LVTLAVLIAALGPALSAVPLHALRLVVGGLLLVFGLQWLRKAILRASGYKALHDEEQI
                     YADELAA"
     gene            complement(378..>1532)
                     /locus_tag="VGD55_14690"
     CDS             complement(378..>1532)
                     /locus_tag="VGD55_14690"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY0871643.1"
                     /translation="VMDDGWFGSRRDDRAGLGDWYVSSEVWPEGLGPIAEYVRSRGME
                     FGLWFEPEMVNLDSDIARAHPEWVIGTPGRMPPPSRSQQVLDVSQPDAFAYLLDRLSM
                     LVGEYGISYIKWDHNRDIADPVHRGGPRVGRPAIHDQTLATYRLMAQLRDQHPGLEIE
                     SCSSGGSRIDFGVLEHTDRVWASDCIDPYERVRIVSGISTLLPFELIGAHVASGRSHT
                     TGRTHELSFRLAVALFGHAGLEWDLTTTSPEVREQLATWVAFAKSVRQLLRTGELVRV
                     GRPADQETVLYGVVSPRRDEALFNLVRLQTGPRYGTAPVVFAGLDPDLRYHLRRVPMP
                     GDGPPVHGQEKLHDGARDIIVPGSLLMGAGVEAPNLQPEQAAIYHLHAES"
BASE COUNT          281 a          528 c          493 g          230 t
ORIGIN      
        1 cggccgcgag ctcgtcggcg tagatctgct cctcgtcgtg caatgccttg tagccgctcg
       61 ctcgcaggat cgccttgcgc aaccactgca ggccgaacac gagcaacagc ccaccgacaa
      121 cgagccgcag cgcatgcagc gggacggcgg acagtgccgg tccgagcgcc gcgatcagca
      181 ccgcgagggt gaccagcgcg gcaaacaggc cgtagagcgc cgaccgccag tcgcgggccg
      241 tgcctgcggc caagacgatg gtggtggcct cgaccgcctc gacggtgcac gccaggaaga
      301 cggcgaggaa cagcgctcca ccgctcatga gctcttcacg gcctcaagtt tctcacgccg
      361 tgagtgtggt cgatgcgcta gctctccgcg tgcaggtgat agatcgcggc ctgctcgggc
      421 tggaggttcg gcgcttcgac acccgccccc atgagcagcg agcccggcac gatgatgtcg
      481 cgcgcaccgt cgtgcaactt ttcctgccca tgcacgggag gcccgtcgcc gggcatcggc
      541 actcggcgca ggtggtaacg caggtcggga tcaagaccag cgaacacgac tggcgccgtg
      601 ccgtaccggg ggccggtctg cagtcggacg aggttgaaca gcgcctcgtc tcgcctcgga
      661 ctgacaacgc cgtacaacac cgtctcttgg tcggcgggcc gaccgacccg taccagttcg
      721 ccggttcgca acagctgccg caccgacttg gcgaacgcga cccaggtagc gagttgttcg
      781 cgcacttccg gcgatgtcgt cgtaaggtcc cactcgaggc cggcatgccc gaacaacgcg
      841 actgcgagcc ggaacgacaa ctcgtgcgtg cgccccgtcg tgtggctgcg acccgacgcg
      901 acgtgcgcgc cgatcaactc gaacggcaac agggtcgaga tgcctgacac gatgcgaact
      961 cgctcgtacg gatcgatgca gtcgctggcc cacacccggt cggtgtgctc gagcacgccg
     1021 aagtcgatgc gcgagccacc ggacgaacac gactcgatct ccaggcctgg gtgctgatcg
     1081 cgcaactgcg ccatcagccg gtacgtggca agcgtctggt cgtggatggc aggccgaccg
     1141 actcgcggac cgccgcgatg cacggggtcg gcgatgtcgc gattgtggtc ccacttgatg
     1201 tagctgatgc cgtattcgcc aacaagcatc gacaatcggt cgagcagata cgcgaacgcg
     1261 tcaggttgag acacgtcgag cacttgctga ctgcgagatg gtggcggcat ccgacccggc
     1321 gtgccgatca cccattccgg atgcgcacgc gcaatatcgg agtcgaggtt gaccatctcg
     1381 ggctcgaacc acaggccgaa ctccatgcca cgcgaccgca cgtactcggc gatgggacca
     1441 aggccctcag gccaaacttc gctcgacacg taccagtcac ccaggccggc gcggtcgtcg
     1501 cgccgcgacc cgaaccaccc gtcgtccatc ac
//
LOCUS       DASWVF010000830         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_23856, whole genome shotgun sequence.
ACCESSION   DASWVF010000830 DASWVF010000000
VERSION     DASWVF010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23856"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VGD55_14695"
     CDS             complement(<1..685)
                     /locus_tag="VGD55_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871644.1"
                     /translation="MGSALKSRRWLSGRAATGACLLAVVAVTLAACASPKPIKFVAEG
                     SVPAAPVSSPPPTQKQSTLALLALLQDRTGPPAPGYTVDQFGSTLRAVSGSGGCNSRD
                     FILKRDLIGLTYQTGSTCIVNSGVLFDRYTGQWFWYSKREAVHLVATDDVVSLADAWA
                     NGASTWSATQRSEFVNDPEELIETSTAAVAAKAGRSADLWLPTDVGMRCVYVADQITV
                     KSAYLLTVTP"
     gene            complement(822..1175)
                     /locus_tag="VGD55_14700"
     CDS             complement(822..1175)
                     /locus_tag="VGD55_14700"
                     /inference="COORDINATES: protein motif:HMM:NF036408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HEY0871645.1"
                     /translation="MPRIEGDPGPLRWLRYAVGFRLPAANREWVAHDLTDAGWRTRAA
                     VRQLVLLVPVAAVFAALPGPWSLRLLLMALVLVGGLLVAAMYGDSVRASRLRQHGLPV
                     PEDRDLGRPTDSAQG"
     gene            complement(1201..2079)
                     /locus_tag="VGD55_14705"
     CDS             complement(1201..2079)
                     /locus_tag="VGD55_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871646.1"
                     /translation="MLAGTSCQVFPADNYWHANIASLPVDVHSAAWLSAMGASTRKLH
                     PDFGPSGDTAVPYGIPVTVVPAGQKRVPVTFQYADESDKGPYPLAANTLIEGGAGSGG
                     DMHAIMVTADTCQLFETWDTLDTSSGWTAGSGAVWSLSSDALRPNTWTSADAAGLPIL
                     PGLLRYDEVASGHVDHAIRFTTNTTDDSFLWPARHAASSNHNANLPPMGARFRLKASF
                     SLAGYSAQAQTVLRAMQTYGLVLADNGSPWYFQGTADNAWPDALIEALKTIPASAFEA
                     VDTSSLEISANSGAAR"
     gene            2096..2557
                     /locus_tag="VGD55_14710"
     CDS             2096..2557
                     /locus_tag="VGD55_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871647.1"
                     /translation="MTGGGAGVVGFGAVVGLVGVAAAVVGFVAGFVVVAFAGLVVSLA
                     LVLDGVPAAVDAAVDAAADAVADDARLDPGRLDPALEAAAVLALLTTLLSGGAARWWP
                     VLQAAVMSASAAMAVAIPVAPKELRVRFIPHLRPRRWRSEAVGALDVPISP"
     gene            2579..3568
                     /locus_tag="VGD55_14715"
     CDS             2579..3568
                     /locus_tag="VGD55_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871648.1"
                     /translation="MSWQMDDGLHCGWPARHRQYLAMTSTTLMTTAGIRLESLTKSYG
                     PVLAVAGIDLVIAPGETVALLGPNGAGKSTTIDMILGLAKPDTGSVQVFGMTPAEAVR
                     AGAIGAMLQTGQLLRDLTVRELIAMMASLYPNPMGVDDVLSVTGIEDIANRRTQKLSG
                     GQTQRVRSAIALVSNPDLLILDEPTVAMDVEVRREFWTSMREFASRGKTVVFATHYLE
                     EADSYADRIVLMARGRIVADGPATEIKATVGLHTIRATLPDVPIPTLTALEGVQSGER
                     RGEAVVLHCTDSDAAIRALLRDFPVASDIEIAGAGLEEAFLLLTGDDKSEELS"
     gene            3565..4293
                     /locus_tag="VGD55_14720"
     CDS             3565..4293
                     /locus_tag="VGD55_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871649.1"
                     /translation="MNPTYLKYELIRAVRNKRGFIISLVFPLIFFYLIAGGNRHQQLG
                     GIPFPTYYLAGMASFGTMGAMIALGARISAERSVGWTRQLRLTPLSVRNYIRAKVLTG
                     YMTALISLALLFVAGATLGVHLTPKGVLHMTGLIVVALIPFAALGILIGHLFTPDSMG
                     PVIGGGVSILAFLGGAWGPVGGDHGPLHDISQGTPTYWLVQAGHTLVGAPAWDSKGWI
                     VVAIWAVVLGALAAWAFRRDTSRI"
     gene            4307..>4654
                     /locus_tag="VGD55_14725"
     CDS             4307..>4654
                     /locus_tag="VGD55_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871650.1"
                     /translation="MSVSPHTTATTREVARRLGGPGNDREEGPKRWRRGGGFWLIYLA
                     VPLGSAWTHHQLVASIAGTVLLVAFGWAYLFLVPLGWWGARGARYSYLIVGVLMTLTV
                     AATAVIGENGLMAL"
BASE COUNT          748 a         1481 c         1603 g          822 t
ORIGIN      
        1 ctggggtcac cgtcagcagg taggccgact tcaccgtgat ctgatcggcg acatagacgc
       61 accgcatgcc gacgtcggtt ggcagccaga ggtcggcact acggccggcc tttgctgcga
      121 cggccgcggt cgaggtctcg atcagctctt cgggatcgtt gacgaactcc gagcgctgcg
      181 tggctgacca ggtcgatgcg ccgttggccc acgcatcggc gagagacacg acgtcgtcgg
      241 tcgcgacgag gtgcacggcc tcgcgcttgg agtaccagaa ccattgaccg gtgtagcgat
      301 cgaacagcac accgctgttg acgatgcagg tgctgccagt ctggtaagtg agcccgatga
      361 ggtcgcgctt caaaatgaag tcccgcgagt tgcagccgcc gctgcccgag accgcgcgca
      421 acgtcgaacc gaactggtcg acggtgtagc ccggtgcggg cgggccagtg cggtcttgaa
      481 gcagcgcgag cagtgcgagc gtggattgct tttgagtcgg aggcggcgac gaaacgggtg
      541 ccgcaggcac gctgccttca gccacgaact tgatcggctt cgggctagcg caggcggcga
      601 gcgtcacggc cacgacggcg agcaggcacg ccccggtggc cgcacgtccc gagagccaac
      661 gtcgcgattt caatgctgat cccacgctgc cctccccggt aagcgtccct gtacacaatt
      721 cgtacacaat tgctcgctta cagggggcgt taccctccgt agtcggccag ccgccagagt
      781 agcgctgttt gtcgggtcga attggcaaga ccgcagatct gctacccctg cgcagaatcg
      841 gttggccggc ccaggtcgcg atcctcggga acgggcaatc cgtgctgccg aagtcgcgac
      901 gcgcgcacgc tgtcgccgta catcgcggcc accagcaggc cgccgaccag caccagcgcc
      961 atcagcagca ggcgtagcga ccacgggcca ggcaaggcgg caaagacagc agccacgggt
     1021 acgagcagaa cgagctggcg aaccgccgcc cgggtgcgcc agcccgcgtc tgtcaggtcg
     1081 tgcgcgaccc actcacgatt ggcggccggc agccggaagc cgacggcgta gcgcaaccag
     1141 cgcaacgggc cggggtcgcc ttcgatgcgg ggcacgcacc gaccgtagaa cagttgcgtg
     1201 ctagcgcgct gcgccgctgt tcgcggagat ctcgaggctg ctggtgtcga cggcttcgaa
     1261 ggcgctcgcg gggatcgtct tcagtgcttc gatcagcgcg tcgggccagg cattgtcggc
     1321 ggtgccctgg aagtaccacg gcgagccgtt gtcggcgagc acaagaccgt aggtctgcat
     1381 ggcgcgcagc acggtttggg cctgtgccga gtagcccgcg agggagaagc ttgccttcag
     1441 ccggaaacgc gcacccatcg gcggcaggtt cgcattgtgg ttcgagctcg cggcatgccg
     1501 agcgggccac aagaaactgt cgtcggtcgt gttcgtggtg aacctgatcg cgtggtcgac
     1561 gtgcccggag gcgacttcgt cgtagcgcag caaccctggc aagatcggca accctgcggc
     1621 gtccgctgaa gtccatgtgt tcggacgaag cgcatcgctc gagagcgacc acaccgcgcc
     1681 cgaacccgcg gtccacccgg acgacgtgtc gagggtgtcc caggtctcga acagttggca
     1741 ggtgtccgcg gtcaccatga tcgcgtgcat atcgccgccc gatcccgcgc caccctcgat
     1801 caaggtgttc gccgcgagtg gatagggacc cttgtcgctc tcgtccgcgt attggaaggt
     1861 caccggcact cgcttctgac cggccgggac gacggtgacc gggatgccgt acggaaccgc
     1921 tgtgtcgccc gacggtccga agtcggggtg cagcttgcgc gtcgatgccc ccatcgccga
     1981 gagccacgcc gcactgtgga cgtcgaccgg cagcgacgcg atgttggcgt gccagtagtt
     2041 gtcggccggg aagacctggc aactcgtacc ggccagcacg tcagcgccgg ctgccttgac
     2101 cggtggtggg gcgggagtcg tcggcttcgg tgccgtcgtc ggcttggtcg gcgtcgcggc
     2161 cgcggtcgtg ggtttcgtgg ccggcttcgt tgtggtcgct ttcgcagggc tcgtcgtgtc
     2221 cttggcgctc gtgctcgacg gcgttccagc cgcagttgat gcggcagttg atgcggctgc
     2281 cgatgcggtc gccgatgacg cgaggctcga cccaggcagg ctcgacccgg cactcgaggc
     2341 ggctgccgtg ctcgcgctgc tcaccacgct gttgtcgggt ggcgcagccc ggtggtggcc
     2401 ggtgctacag gccgcggtga tgagcgcgag cgcggcgatg gcggtggcga ttccggtggc
     2461 cccgaaggag ctgcgcgtcc gtttcatacc tcatcttcga ccccgcaggt ggcgatccga
     2521 agccgtcggc gccctggatg taccgatttc gccctgacgt cggcgacggg ggtgacgcat
     2581 gtcatggcag atggacgacg ggttgcactg tggctggccc gcccggcacc ggcagtatct
     2641 ggctatgaca tctacgaccc tcatgacgac agcggggatc cgcctggagt cattgaccaa
     2701 gagctatggc ccagtactgg cggtcgccgg catcgacctg gtcattgcgc ccggggagac
     2761 ggtcgcgttg ctcggaccaa acggggccgg caagtcgacg acgatcgaca tgattcttgg
     2821 gctggcgaag cccgatacgg ggtcggtgca ggtgttcggc atgacacccg ccgaggcagt
     2881 tcgcgcgggc gcaatcggcg cgatgctcca gactggccag ctgctgcgcg acctcacagt
     2941 gcgcgagctc atcgcgatga tggcgtcgct gtatccgaac ccgatgggtg tcgacgacgt
     3001 gctaagcgtc accggcatcg aggacattgc taatcgtcgc acccagaagt tgtcgggtgg
     3061 gcagacgcag cgcgtgcggt cggcgatcgc gttggtctcc aaccccgacc tgctgatcct
     3121 tgacgaacca acagtcgcga tggacgtcga ggtgcgccgc gagttctgga cctcgatgcg
     3181 cgagttcgcg tcgcgcggaa agactgtggt gttcgccacg cactacctcg aagaggcaga
     3241 cagttacgcc gaccggatcg tcttgatggc gaggggccgt attgtggccg acgggcccgc
     3301 gaccgaaatc aaggcaaccg tcgggctgca cacgatcagg gcgaccttgc ccgacgtccc
     3361 gattcccact ttgaccgcgc tcgaaggagt gcaaagtggc gaacgacgtg gtgaagccgt
     3421 tgtgctgcac tgcaccgatt ctgacgcggc tattcgcgca ctgcttcgcg actttcccgt
     3481 cgcaagtgac atcgagatcg cgggtgctgg cctggaagaa gcgttcctgt tgctgaccgg
     3541 cgacgacaaa tccgaggaac tgtcatgaac ccgacctacc tgaagtacga gttgatccgc
     3601 gcggtccgca acaagcgcgg cttcatcatc tcgctcgtgt tcccgctcat cttcttctac
     3661 ctgatcgccg gcggtaacag gcatcagcaa cttggcggga ttccgttccc gacgtactac
     3721 ctcgcaggca tggcgtcgtt cgggacgatg ggcgcgatga tcgcactcgg cgcgcgcatc
     3781 tcggccgagc gctcggtcgg ctggacccgc cagttgcggc tgacacccct gagtgttcgc
     3841 aactacatca gggcgaaagt gctcaccggt tacatgaccg cactgatctc actcgcgctg
     3901 ttgttcgtcg cgggcgccac gctgggtgtg cacctcacac caaaaggcgt cctgcacatg
     3961 accggcctca tcgtcgtcgc actgatcccg ttcgcggcac tgggcatctt gatcggtcac
     4021 ctgttcaccc ccgattcgat ggggccggtg atcggaggtg gcgtttcgat cctcgcgttt
     4081 ctgggcggcg cctggggccc ggtcggcggc gaccacggac cgcttcacga catctcgcag
     4141 ggaacgccga cctactggct ggtgcaggcc ggccatacat tggtgggggc gccggcgtgg
     4201 gactccaagg ggtggatcgt ggtcgcaatc tgggccgtcg tgctcggcgc gcttgccgcg
     4261 tgggccttcc ggcgcgacac gtcacggatc tgagcggacg acgtcaatga gtgtcagtcc
     4321 tcacacgacc gccaccaccc gagaagtcgc gcggcggttg ggcgggcccg gcaatgaccg
     4381 ggaggagggc cctaagcgct ggcgccgagg cggcgggttc tggttgatct atctcgcggt
     4441 gccgctgggc agtgcgtgga cccatcacca actcgtcgcc agcatcgccg gcacggtctt
     4501 gttggtggcg ttcgggtggg catacctgtt cctcgtgccg ctgggctggt ggggtgcccg
     4561 cggcgctcgc tacagctatc tgatcgtcgg cgtgctcatg acactgacgg ttgcggcgac
     4621 cgcggtcatc ggcgagaacg gtctgatggc gctg
//
LOCUS       DASWVF010000831         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_507946, whole genome shotgun sequence.
ACCESSION   DASWVF010000831 DASWVF010000000
VERSION     DASWVF010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507946"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VGD55_14730"
     CDS             <1..919
                     /locus_tag="VGD55_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEY0871651.1"
                     /translation="DTAGYQVGGFPARWSEWNGKYRDDVRDFWRGEGAIGDFGLRLTG
                     SGDIYPSDRRTPTASINFVTAHDGFTLADLTSYNDKHNDANGEDNNDGETNNSSWNCG
                     VEGPTDDDSINELRARQRRNLLGTLLLSAGVPMILGGDEIGRTQDGNNNAYCQDNEVS
                     WYDWDNIDENLLAFTKTALKLRRENPALRPRDYLRGPGGLPAQMVLYKADGVEMSDED
                     WKDTGTKTLAVALDGRQIEDADGDTTRDRFLLLVNAHHEPVDFTVPAGGRRPWRVVLT
                     TDNPDPIEPVSSGDVLTVLDRSILLLHTT"
     gene            complement(996..>1796)
                     /locus_tag="VGD55_14735"
     CDS             complement(996..>1796)
                     /locus_tag="VGD55_14735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0871652.1"
                     /translation="NPVDWKIRSGAYGPTSPDNLPTVFGFEASGVVRAVGKDVEGFAV
                     GDEVFGRAATGSGTWAEFTLLSADGTAHKPPHVSFNDAATLSIAGATAYDGVTQLELK
                     PGQTLLINGASAGVGVAAAQIARDLDINVIGTASEEKRALVESLGATFIAYGDGVADR
                     VRELMPDGVDAILDLAGGDGLRAVAELVADKTKLISAADPATAAEVGGHAIVRDYDRG
                     ILDVLAAMVGDRKLDPHVEDVVPLDKAADALAAVEGGHAKGKVVIAVG"
BASE COUNT          329 a          608 c          561 g          298 t
ORIGIN      
        1 ggataccgcc ggctaccaag tcggcggttt cccggctcgg tggtcggagt ggaacggtaa
       61 gtatcgcgac gacgtacgcg acttctggcg cggcgagggg gcgatcggcg acttcggttt
      121 gcggctcacg ggcagtggcg acatctaccc ctctgatcgc cgaacaccga cggcaagcat
      181 caacttcgtg accgcccacg acggcttcac ccttgccgac ctcacgtcgt acaacgacaa
      241 gcacaacgac gccaacgggg aagacaacaa cgacggcgag acgaacaaca gctcctggaa
      301 ttgcggtgtc gaagggccga ccgacgacga ctcgatcaac gagctgcgtg cccgtcagcg
      361 tcgtaacctg ctcggcaccc tgttgctctc agccggtgtg ccgatgattc tcggcggcga
      421 cgagatcggc cgcacgcagg acggcaacaa caacgcctac tgtcaggaca acgaggtgtc
      481 gtggtacgac tgggacaaca tcgacgagaa cctgttggcc ttcacgaaga cggccttgaa
      541 gctgcgccgc gagaacccgg cgttgcggcc acgagactac cttcgtgggc cgggtggtct
      601 gcctgcgcag atggttctgt acaaggccga cggcgtggaa atgtctgacg aggactggaa
      661 ggacaccgga accaagacgc tcgcggtggc acttgacggt cggcagatcg aagatgccga
      721 cggcgacacg acgcgtgacc gcttcttgct tctcgtcaat gcccaccacg agccagttga
      781 cttcactgtc ccggcgggcg gccgtcgccc gtggcgcgtc gtgctcacca cagacaaccc
      841 cgacccgatc gaaccggtct ccagcggcga cgtactaact gtgctcgacc gttcgatctt
      901 gctcctgcac acgacctagg ccttgaagtc cccgacgtcc gccgcgcaaa ggaaccctgc
      961 cgtccgctgc gcaaagactt aacaaccgcg cgtggttatc cgactgcgat gacgaccttg
     1021 cccttggcgt gaccgccctc gactgctgcc aacgcatcgg ctgctttgtc gagtgggacg
     1081 acgtcttcga cgtgcgggtc gagcttgcgg tcaccgacca tcgccgccag cacgtcgagg
     1141 attccgcggt cgtagtcgcg cacgatcgcg tggcccccga cctcagctgc ggtcgctggg
     1201 tcagccgcgc tgatcagctt ggtcttgtcg gctaccagtt cggcgactgc tcgcaaaccg
     1261 tctccgccag caaggtcgag gatcgcgtca accccgtcgg gcatcagttc cctgacccgg
     1321 tcggcgaccc cgtcgccgta ggcaatgaag gtcgcgccga gcgactccac gagcgcccgc
     1381 ttttcctcgc tggcagtgcc aatcacgttg atatcgaggt cgcgggcgat ctgcgcggct
     1441 gcgacgccga ccccggcgct ggcgccgttg atgagcaacg tctggcccgg cttgagctcg
     1501 agttgggtga caccgtcata ggcagtcgcc ccggcgatcg acaacgtcgc ggcgtcattg
     1561 aacgagacgt gcggcggctt gtgcgcagtg ccgtcggcgc tcagcaaggt gaactcggcc
     1621 caggtgcccg aaccggtcgc ggcacggccg aacacctcat caccgacagc gaacccctcg
     1681 acgtccttcc ctacagcgcg aaccacgcct gacgcctcga agccgaacac cgtcggcaag
     1741 ttgtccgggg acgtcggccc gtaggccccg gagcggatct tccagtcgac cgggtt
//
LOCUS       DASWVF010000832         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_278733, whole genome shotgun sequence.
ACCESSION   DASWVF010000832 DASWVF010000000
VERSION     DASWVF010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278733"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VGD55_14740"
     CDS             complement(<1..1255)
                     /locus_tag="VGD55_14740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0871653.1"
                     /translation="MTEKSGIRTSPRRALHVEPGRRAHDIMRFVYAFLGLLLAAYLIS
                     WLFRTQPSSPLDDWWVAGFEVVAAGLCMVKALPRRPGRAMPLILGLALLSWALGDIVL
                     AIESLGGAEPSTPSFADAFYVTFYPLAYVAIVIFMRGQVRRLSTPSWLDGAVAAAGAA
                     AVCAAFEFERILHSTGGDKLATATNLVYPIGDLLLFSLVVGGSAIMSGQRKTPLILMA
                     GGIAFNIAGDTANLFSASAWPAHLGVVLDAIAWPSSLLLLSMSVWFRPRPLNPLVLQK
                     PTGFVIPGLCAASALAVLFVGGLRSVSLVALYLATATMLLVGIRLLLSMRSMRSLSQE
                     RRRLSFTDELTGLGNRRHLFSVLEAFFTDPGTTAEPRMLAFLFVDLDHFKEINDAFGH
                     SAGDALLTQLGVRLSACMRDTDLLVR"
     gene            complement(1329..>1645)
                     /locus_tag="VGD55_14745"
     CDS             complement(1329..>1645)
                     /locus_tag="VGD55_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871654.1"
                     /translation="VFAVTNAAGHTTVVNLKTGAISAPTATTVGWCVENGTYNLTGMD
                     QQGGTPVDFTSGDLAAPCTESGAPSHPADGTAANAGVTVGGYFVWPSLDGLHAFKIGV
                     PT"
BASE COUNT          336 a          511 c          548 g          250 t
ORIGIN      
        1 gccgcaccag gaggtcggtg tcgcgcatgc acgctgaaag acgtactccg agttgggtga
       61 gtagcgcgtc gcccgctgag tgcccgaatg cgtcgttgat ctccttgaag tgatcaaggt
      121 cgacgaacag gaaggcaagc atgcgtggtt cggccgtcgt accgggatcg gtgaagaaag
      181 cctcaagcac gctgaagagg tggcgtcgat tgccgaggcc cgtcagttcg tcagtgaaag
      241 acagccgccg acgctcttgc gatagcgacc gcatgctgcg catcgacagc agcagccgaa
      301 tgcccaccag caacatcgtt gcggtggcga gatagagagc gacgaggctc acgctgcgaa
      361 gaccgccaac gaacagcacc gcgagggcgc tggcggcaca cagcccggga atgacgaagc
      421 cggttggctt ttgcagcacc aacgggttca acggccgcgg gcggaaccag accgacatcg
      481 acaacaggag cagtgagctc ggccacgcga tcgcgtcgag aacgacgccg aggtgagcgg
      541 gccacgccga agccgaaaag aggttggcgg tgtcgccggc gatgttgaac gcaatgccac
      601 ctgccatcag gatcaggggt gtctttcgct ggcccgacat gatcgccgag ccgccgacca
      661 cgaggctgaa cagcagcagg tcgccgatcg gatacaccag gttggtcgcc gtcgcgagtt
      721 tgtcgccgcc ggtcgagtgc aggatgcgtt cgaactcgaa tgccgcgcaa accgccgccg
      781 cgccagccgc tgccaccgcg ccgtccagcc agctgggcgt cgagagtcgg cgaacctggc
      841 cgcgcatgaa aatgacgatc gccacgtacg ccagcggata gaacgtgacg tagaacgcgt
      901 cggcaaacga cggcgtcgag ggctctgcac cgccgaggga ttcgatcgca agcacgatgt
      961 cgccgagtgc ccacgacagc agggccagtc cgaggatcaa cggcatcgca cgcccgggtc
     1021 gcctgggcag cgccttcacc atgcacaacc cggcggccac gacctcgaac ccagcgaccc
     1081 accaatcgtc gaggggcgag gagggctgcg tcctgaagag ccacgagatc aggtaggccg
     1141 cgagcaggag gccaaggaat gcgtagacga accgcatgat gtcgtgcgcg cgtcggcccg
     1201 gctcgacgtg caacgcgcgg cgaggtgatg ttcgtatccc ggatttctcc gtcacgtctg
     1261 ccgatcggcg atctgacgca ctacctgatg tctttaccaa cccgagcatg gcacgcgccg
     1321 ggcggtgctc aggtcggcac gccgattttg aacgcgtgca gtccgtcgag cgatggccag
     1381 acaaagtagc caccgacggt cacccctgca ttcgccgcag ttccatcagc gggatgagag
     1441 ggcgctccgc tttccgtaca aggtgcggcg aggtcgccgg atgtgaagtc gacaggcgtg
     1501 ccgccttgtt ggtccatgcc agtgaggttg taggtgccgt tctcgacgca ccatccgacc
     1561 gtggtcgcag tcggcgcgga gatcgcccct gtcttgagat tgaccacagt tgtatggcct
     1621 gcagcgttgg tcacggcaaa cacat
//
LOCUS       DASWVF010000833         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_125505, whole genome shotgun sequence.
ACCESSION   DASWVF010000833 DASWVF010000000
VERSION     DASWVF010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125505"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VGD55_14750"
     CDS             complement(<1..217)
                     /locus_tag="VGD55_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871655.1"
                     /translation="MPLPVPTTPAGVEGLRSILAQPQRALVGVDFDGTLAPIVADPAM
                     ARAHPGAPDVVRRLAGSIRAVAVVTGRP"
     gene            complement(228..878)
                     /locus_tag="VGD55_14755"
     CDS             complement(228..878)
                     /locus_tag="VGD55_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEY0871656.1"
                     /translation="MTEPETRLAGSHRRNSSARRVRLLVSLLVVVLAAGGVLAALHLR
                     GESSKPKTPVAASSHTSSLRPPTGSPSASVSVSPVPSLSAPVIVSNPPPVVSTSPAST
                     SPASVSPASVSPAPSTSASVARPSVDILNDTHISGLAASAAKTLTSGGWTVAETGNYP
                     HSVSTTTVFYPPDQVDAAKQLAQQYGAIREVLAAPSGVSTTDLTLVLATDWTTDGK"
     gene            complement(941..1270)
                     /locus_tag="VGD55_14760"
     CDS             complement(941..1270)
                     /locus_tag="VGD55_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEY0871657.1"
                     /translation="MEVANSADAAHAAGVTAAMSEPGIEPDALGERDLLILEFERQWW
                     KYAGAKEQAMREKFDMSATRYYQALNALIDRPAALEADPMLVKRLRRLRAARQRARSA
                     RRLGIDA"
     gene            1359..2219
                     /locus_tag="VGD55_14765"
     CDS             1359..2219
                     /locus_tag="VGD55_14765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0871658.1"
                     /translation="MSVAAGGGVRGALAQVASWPASDPAAGVTDATDTIAQTGAIDHR
                     HEWASVTKLVTALCVLRLSDAGSVSLDEPAGPPGSTVRHLLAHASGLGFEGTDVLAKP
                     GQRRVYSNAGFDLLGALASERTGLPFAECAAATVFGPLAMSHSAIEGTPSAGGVGPLA
                     DLLRLCRELLAPALVTPGTLAAATTVAFPGIDGVLPGFGRQNPNDWGLGFEIRDHKSP
                     HWTGTANSPATFGHFGKAGSFLWVDPVAGLACAALAGVPFGAWAIEGWPKLSDEVLAQ
                     YGSYPTRPVM"
     gene            complement(2195..>2844)
                     /locus_tag="VGD55_14770"
     CDS             complement(2195..>2844)
                     /locus_tag="VGD55_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY0871659.1"
                     /translation="KSTFLRSLNRLHEVIPGAHITGQVLLDDRDIYAPGVDPVALRRS
                     VGMVFQRPNPFPTMSIYDNVAAGLKLGGSKGKDLDVVVERALTGAHLWAEVKDRLKKP
                     ALALSGGQQQRLCIARAIAVEPEVLLMDEPCSALDPISTLAIEDLIAELKDRYTIVIV
                     THNMQQASRVSETTGFFTLAGAGKPGVLVEYDATSRIFSVPADKRTEDYITGRVG"
BASE COUNT          437 a          960 c          990 g          457 t
ORIGIN      
        1 cgggccggcc ggtcacgacg gcgaccgcgc gaatcgaccc ggcgagccgg cgcaccacat
       61 ccggggcacc gggatgtgca cgtgccattg ccgggtcggc gacgatcggc gcgagggtgc
      121 cgtcgaagtc gacccccacc aacgcccgct gcggctgcgc aaggatcgag cgcaaacctt
      181 caacgcccgc gggagtggtg ggaacgggga gcggcacgcc tcggacccta cttgccgtcg
      241 gtggtccaat ccgtcgcgag caccagcgtg aggtcggtcg tcgagacgcc gctcggcgca
      301 gcaagcacct cgcggatcgc gccgtattgc tgggcaagtt gtttggccgc gtcgacctgg
      361 tcgggcgggt agaagactgt ggtggtcgaa accgagtgcg ggtaattacc ggtctcggcg
      421 acggtccagc cgcccgatgt cagcgtcttc gccgcgctag ccgcgagacc gctgatgtgg
      481 gtgtcgttga ggatgtcgac cgacggccgg gcgacggagg cggacgtgct cggcgccggc
      541 gacaccgacg cgggcgacac cgacgcgggg gacgtcgatg ccggcgacgt cgatacaacc
      601 gggggcgggt tcgaaacgat gaccggagcc gatagcgacg gtaccggcga caccgagacg
      661 gatgcgctcg gcgaaccagt cggcgggcgc aacgacgagg tgtgcgacga cgcggcgacc
      721 ggagtcttcg gcttgctgga ttcgccccgc aggtgcagcg ccgccagcac gccgcccgca
      781 gccaggacca ccactagcag cgaaacgagc aacctcaccc gacgagcact gctatttcgc
      841 cggtggctgc cagctaggcg ggtctctggt tccgtcatgg ggtgtctttc cgtgaaccgg
      901 cccgcgtcag gccatgatca tcgcactgcc gaatgcgtgg ttaggcgtct atacccaggc
      961 gacgcgccga gcgagcgcgc tgccgagcgg cgcgcagtcg gcgcagcctc ttgaccaaca
     1021 tggggtcggc ttcgagcgcg gcggggcggt cgatgagcgc gttcaatgcc tggtaatagc
     1081 gcgtcgcgga catgtcgaac ttctcgcgca tggcctgctc cttggcaccg gcgtacttcc
     1141 accattggcg ctcgaactcg aggatcagca ggtcgcgctc gcctagtgca tcgggctcga
     1201 taccaggctc agacatggcg gctgtcacgc ccgcggcgtg cgcggcgtcg gcgctgttcg
     1261 cgacctccac aggccagccc cttcctacct cggtgttcgg ccccatcgta ggacccaaac
     1321 aacaccgatg tcattctccc gcaccctacc ctggtggcat gtcggtagcg gctggtggag
     1381 gggttcgcgg cgcgcttgcg caggtcgcca gctggccggc gtccgacccc gccgcaggcg
     1441 tgaccgacgc gaccgacacc atcgcgcaga ccggcgccat cgatcatcgc cacgagtggg
     1501 cgtcggtcac caagctggtc accgccctgt gcgtcttgcg cctcagcgac gcgggttcgg
     1561 tctccctcga cgagcccgcc gggcctcccg gctcgaccgt acgccacctg ctcgcccacg
     1621 cgtcgggcct gggcttcgag ggcaccgacg tcctggccaa gccgggacag cgtcgggtct
     1681 actccaacgc cggattcgac ctgctcggcg ccctcgccag cgaacgcacc ggattgccgt
     1741 tcgccgagtg tgccgctgcg accgtgttcg ggccgttggc aatgagccat tccgcaatag
     1801 aggggacgcc gtcggcgggc ggtgtcgggc ccctcgccga cctgcttcgc ctctgccgcg
     1861 aactgctggc gccggcgctc gtgacacccg gaacgctggc cgcagcgacc accgtggcgt
     1921 tcccggggat cgacggagtg ctgcccgggt tcggccgcca gaacccgaac gactgggggt
     1981 tggggttcga aatacgcgat cacaagtcgc cgcactggac aggcacggcg aactcccccg
     2041 ccaccttcgg gcacttcggc aaggcgggca gcttcctgtg ggtcgatccg gtcgcgggtc
     2101 tcgcgtgcgc ggcgcttgca ggtgtgccgt ttggtgcgtg ggcgatcgag ggctggccga
     2161 agctctccga cgaggtgctc gcgcagtacg ggagctaccc gacgcgaccg gtgatgtagt
     2221 cctcggtgcg cttgtcggcg ggtaccgaga agatgcggct cgtcgcgtcg tactcgacaa
     2281 gcactcccgg cttacctgcg cccgcgagcg tgaagaagcc cgtggtctcg ctgacccggc
     2341 tcgcctgctg catgttgtgg gtcacgatca cgatcgtgta gcgatccttg agctcggcga
     2401 tgaggtcctc gatcgccagc gtcgagatcg ggtcgagggc cgagcagggc tcatccatca
     2461 gcagcacttc aggctcgacc gcgattgcgc gtgcgatgca gagccgctgc tgctggccac
     2521 cggacagtgc cagcgccggc ttcttcagcc ggtccttgac ctcggcccac aagtgggcac
     2581 cggtaagggc gcgctcgacc accacgtcga ggtcttttcc cttgctaccg cccaatttca
     2641 ggccagcagc gacgttgtcg tagatcgaca tcgtggggaa aggattcggc cgctggaaca
     2701 ccatgccgac gctgcggcgc agtgctaccg ggtcgacacc aggggcatag atgtcgcggt
     2761 cgtcgagcag cacctgcccg gtgatgtggg cgccagggat gacttcgtgc agccggttga
     2821 gactgcgtag gaacgtcgac ttgc
//
LOCUS       DASWVF010000834         6167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_328930, whole genome shotgun sequence.
ACCESSION   DASWVF010000834 DASWVF010000000
VERSION     DASWVF010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328930"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VGD55_14775"
     CDS             <1..1156
                     /locus_tag="VGD55_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871660.1"
                     /translation="LLGGPRLPPTTAAAAGTEAFGALPHLPHLLHKVISVFAYPLISG
                     IANAVIKDREMHHHPYELRVIGDTEAADVVADQLTREQWEALSGEPCLLLLHGIFGTS
                     RGTFTGVLDGNPKQSLPSMIPALRAVYGNRILAFDHPTVSVGPQENAQQFLSRVPADL
                     PFRFDVLVHSRGGLVARCVAGGAGLDPQQVSFRKGVFVGTPNDGTEIAAPGLIAQLVD
                     RMTSFLHFVPPVGPPGTVAAVLTSVLEVVKVFGQGIEGGMPGLTDMQPGSPLLTALNH
                     PATPPQTPPYFEIDADYRPDGSLRWLMRGFDDVEDDAVFGKAANDVAVPSAGVGETTV
                     GAGETVAGPGFPVPETAALHYPAGVVWHCTYFEQAQTHAHLRDWLLTTAQ"
     gene            complement(1140..2210)
                     /gene="dapE"
                     /locus_tag="VGD55_14780"
     CDS             complement(1140..2210)
                     /gene="dapE"
                     /locus_tag="VGD55_14780"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0871661.1"
                     /translation="MTLDIDSGVASLTAALVDVPSVSGDEQAIADEVETALRALAYLD
                     VERDGNVVIARTDTGAAQRIVLAGHLDTVPIAANVPSRRDGTILHGCGTSDMKSGVAV
                     MLRAAAMLRHAPALYDLTYVFYDCEEVEAARNGLGRLARTRPSVLEADLAIVLEPTNG
                     AVEAGCQGTLRADITLTGIRAHSARSWLGRNAIHAAAPVLQRLADYEPRSVEIDGCVY
                     REGLSAVGIKGGVAGNVIPDACTVSINFRFAPDRDIEAAEQHVRVVFPGYDVVITDAA
                     PAAMPRLGEPAAAAFVAAIGAPPSAKYGWTDVARFAELGIPAVNYGPGDPDVAHTRGE
                     SVDLSVVERVERSLLGYLTAPL"
     gene            complement(2214..3050)
                     /locus_tag="VGD55_14785"
     CDS             complement(2214..3050)
                     /locus_tag="VGD55_14785"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0871662.1"
                     /translation="MDLPLSSPLPAEIDSLWERRADLSPADATAAAVVIEAVDLLDAG
                     KARVAWVEPSSDAVVVDERAKRAILLSFKVLAMRESSAGDFRYHDRMPLKTRFDGVRV
                     VPGAIVRWGAFVAPSAVLLPSFVNIGGYVDEGTMVDTWATVGSCAQVGKRVHLSGGVG
                     LGGVLEPPNAVPVVVEDDAFVGSRSMVVDGARVRRGAKLGAGCILTASTHVFDAETGA
                     EFPRGEAPAWSVCVGSTRVKSFPGGDFGMPCLLVLRRLDEGQSHDKLVLNDILREHGV
                     AE"
     gene            3249..4148
                     /locus_tag="VGD55_14790"
     CDS             3249..4148
                     /locus_tag="VGD55_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871663.1"
                     /translation="MLVALALVTTAVIYGLKSLTPHHTVADSCQVVGETTGTIYGMDP
                     EQLQNASLIADVAMRRGLADEAVIVALATAQQESKLRNIDYGDADSVGLFQQRPSQGW
                     GSKEQILDPLYSTGKFFDALVKVRNWQNLTVAAAAQAVQRSAFPDAYTSWQPYGTALA
                     AALTGNSLGQLTCRLGHPGADLKTSGAGSTPTSLVAVVSQLTASLASDLQVTSPEVTS
                     QGTKVATITVSGLGGVGAGNDSAAEHRTATVAAWALAHSPLGITKVVVGNQEWRPDRD
                     GWHPASASTTAAVGTVVMTVAAR"
     gene            4174..5175
                     /locus_tag="VGD55_14795"
     CDS             4174..5175
                     /locus_tag="VGD55_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0871664.1"
                     /translation="MPNTDVRAVQPSADAMLAGATSREPMKTADSLSSASFERVVLDG
                     DRFVVKHLSTSSDWIMRASGDVACRPLWMWRSGTLDTLPDCLDPLIVDIAHDSTTGVT
                     TLLMPDVEELLVPEGAGRLEVEQQHRFLDHMAALHAAFWNWDGPPALTPMANRYTTLT
                     PMTSLVEAELGNTDGVPSYLEGAWRRLIEVSPEIGELALAITADPGPLVDALDQTPST
                     LIHGDWKAGNLGSHLDGRTILIDWQWPGQAAGCVDLGWYLAVNCDRLPESKEATIAAY
                     RDALVRHGVDVGDWFDRQLELALLGAFVQLGWSKTGDPAELKWWVDAVLPTARELLR"
     gene            5172..5987
                     /locus_tag="VGD55_14800"
     CDS             5172..5987
                     /locus_tag="VGD55_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0871665.1"
                     /translation="MTDPADAAFDVRADVRAAYDATGAAWDNGPRRIYDQLAIALLDR
                     CPVPFDHARAVDAGAGTGAATNELVRRGASVTAVDLSAGMLRCLDPKNASLAVGDVVA
                     LPIRTDAVDIAMAAFVLNHLPQPIDALRELARITHPAGAVLVSVFGEAPPHPSKDAID
                     GVARLYGFTAPAWHRRLKEEFEAQTSTPEPLATLARQAGLVDVSARVEHIQPTMTDDD
                     LVGWRTGMAHLAPFIATLPTATAQALADDARAAVVGMPPLDIAMVTLVARPLA"
BASE COUNT         1061 a         2168 c         1974 g          964 t
ORIGIN      
        1 gctgctcggc ggcccacgac taccaccgac gaccgccgcc gccgccggca ccgaggcatt
       61 cggcgcccta ccgcacttgc cccacctgct gcacaaagtg atttcggtct tcgcctaccc
      121 cttgatctcc ggtatcgcga acgccgtcat caaggaccgc gagatgcatc accatccgta
      181 cgaactgcgg gtgatcggcg acaccgaagc ggccgacgtc gtcgcagacc aactgacccg
      241 cgaacagtgg gaagccctca gcggcgaacc ctgtctcctg ctgctgcatg gcatcttcgg
      301 tacgagccga ggcacgttca ccggcgtgct cgacggcaat cccaaacagt cactgccgtc
      361 gatgatcccg gcgttgcggg ctgtctacgg caaccggatc ctggcgttcg accatccgac
      421 cgtatctgtt ggcccgcagg aaaatgcgca gcagttcttg tcgagagtgc ctgccgatct
      481 gcctttcagg ttcgacgtgc tcgtgcacag ccgcggcgga ctcgtcgccc gttgcgttgc
      541 cggcggagct ggcctcgatc cgcagcaggt ctcgttccgg aagggtgtct ttgtcggaac
      601 gccgaacgac ggcaccgaga tcgccgcgcc ggggctgatc gcgcagctcg tcgaccgcat
      661 gacgtcgttc ctgcatttcg tcccacccgt cggcccgcca ggcacggtgg ctgcggtgct
      721 gacgtcagtg ctcgaagtgg tcaaggtatt cggccaaggc atcgagggcg gcatgccagg
      781 cctgaccgac atgcagccag gcagtccact tctgacagca ctcaaccatc cggcgacgcc
      841 cccgcagacg cctccctact tcgagatcga cgccgactac cgtcccgacg gatccctgcg
      901 ctggctcatg cgcgggttcg acgacgtcga agacgacgct gtgttcggca aagcggccaa
      961 cgatgtcgcg gttccgagcg cgggtgtcgg cgaaacgaca gtcggggcgg gcgaaaccgt
     1021 agccggcccg ggattcccgg tgcccgagac ggcggccctg cactaccccg caggagtcgt
     1081 ctggcactgc acctatttcg agcaggcgca gacccacgcg cacctacgcg actggctcct
     1141 tacaacggcg cagtgaggta accgagcaac gaccgctcga cgcgctcgac aacactgaga
     1201 tcgacgctct cgcctcgagt gtgcgcgacg tcgggatcgc ctggaccgta gttcaccgcg
     1261 gggatcccca gttcggcgaa acgcgcgaca tcggtccagc catatttggc cgacggcggc
     1321 gctccgatgg cagcgacgaa cgcggcggcg gccggctccc ccagtcgggg catggcagca
     1381 ggagccgcat cggtgatgac cacgtcgtag ccggggaaga ccacgcggac gtgctgttcg
     1441 gcggcctcga tatcgcgatc tggagcgaac cggaagttga tcgacaccgt gcacgcgtcg
     1501 ggaatcacgt tgcccgcgac gcctcctttg ataccgacgg ccgacaaacc ctcgcgatag
     1561 acacagccgt cgatctcgac ggagcgaggc tcataatcgg ccagccgctg caagacgggg
     1621 gcagccgcgt gaatcgcgtt gcggcccaac cacgaccgtg cgctgtgcgc ccgaataccg
     1681 gtgagagtga tgtccgcgcg caacgttcct tggcagccgg cctcaaccgc tccgttggtc
     1741 ggctcgagca cgatcgccag atccgcctcc aacacgctgg gtcgggtgcg ggcgagccgg
     1801 ccgagcccgt tgcgggctgc ctcgacctcc tcgcagtcgt agaacacgta ggtgaggtcg
     1861 taaagcgcag gcgcgtgacg caacatggcg gccgcacgaa gcattactgc gacacccgat
     1921 ttcatgtcgc tcgtgccgca gccgtgcagg atcgtgccgt cacgtcgaga agggacgttc
     1981 gccgcgatgg gcacggtgtc gagatggccg gccagcacga tccgctgcgc cgcgccggtg
     2041 tcggtgcgcg cgatgacgac attgccgtct ctctcgacat cgagataggc cagcgcacgc
     2101 aacgcggtct cgacctcgtc ggctattgct tgctcgtcac cactcactga cgggacgtcg
     2161 accagtgcgg ccgtcagcga cgcgacgcca gagtcgatgt cgagggtcat cgcttactcc
     2221 gcgacgccgt gctcacgcag gatgtcgttg agcaccagct tgtcgtgcga ctggccctcg
     2281 tcgagccggc gcagcacgag caggcacggc atcccgaaat ccccaccggg aaaggacttc
     2341 acccgggtcg aacccacgca caccgaccag gccggcgcct caccgcgggg aaactcggca
     2401 ccggtctcgg cgtcgaacac gtgggtcgac gcagtcagga tgcagccagc cccgagcttc
     2461 gcgccgcgcc tgacccgagc gccgtcgacc accatcgagc gcgaaccgac aaaggcatcg
     2521 tcttcgacga cgacgggcac cgcattcggc ggctcaagca cgccgccgag tccgacgcca
     2581 cccgacaggt gcacccgctt gccgacctgc gcgcacgatc cgacggtcgc ccaggtgtca
     2641 accatggtgc cctcgtcgac gtagccaccg atgttgacga aactcggcag cagcacagcg
     2701 ctcggcgcga cgaacgcccc ccaacgcacg atcgccccgg ggacgacccg caccccgtcg
     2761 aacctggtct tgagcggcat ccggtcgtgg tagcgaaagt cgcccgccga cgactcgcgc
     2821 attgcgagca ccttgaacga gagcaggatc gcgcgcttgg cccgctcgtc aacgacgact
     2881 gcgtccgacg atggttcgac ccacgcgacg cgcgccttcc cggcgtccag caaatcaaca
     2941 gcttcaatca caactgccgc agccgttgca tcggccgggc tgaggtcggc acgccgttcc
     3001 cagagcgagt cgatctcggc gggcagcggg ctggagagcg ggaggtccat ggccgctgac
     3061 cctaccgaca tcgcccgcgt cgccggccga gcccagccga tatcgggctc gacggcgata
     3121 gattcacgct aacgatgaca gcgtctgatc ggcctgttcg atccgcgcga ccggtgcgac
     3181 gtccgcaggc acgccctcag ggacgggcca gccggagtcg tcgcgtccgg ctgacgcgga
     3241 ccatcgtcgt gctcgtcgcc ctcgcgctgg tgaccaccgc cgtgatctac gggctgaagt
     3301 cgctgacgcc gcatcacacg gtcgccgaca gttgccaggt tgtcggcgag accacgggga
     3361 ctatctacgg gatggatccc gagcaacttc aaaacgcgtc gctcatcgcc gacgtcgcca
     3421 tgcgacgcgg gttggccgac gaggccgtca tcgtggcgtt ggcgactgcg cagcaggagt
     3481 cgaagctgcg caacatcgac tacggggacg ccgattcggt cggccttttc cagcaacggc
     3541 cgagccaggg ctggggatcc aaggagcaga tcctcgatcc gctctactca accgggaagt
     3601 tcttcgatgc gctcgtgaag gtacggaact ggcagaacct caccgtcgcc gcggccgcgc
     3661 aagcggtgca acgcagcgct ttccccgatg cgtacacgtc gtggcagccg tacggcaccg
     3721 cacttgctgc cgctttgaca ggcaacagcc tgggtcagct cacctgccgg ctcgggcacc
     3781 ccggcgccga tctgaagacg agcggcgcgg gctcgacccc gacgtcgttg gtcgccgtgg
     3841 tctcacaact cacggcttcc cttgccagcg accttcaggt gacctcgccg gaagtgactt
     3901 cccagggcac gaaggtggca accatcacgg tgtctggctt gggcggagtc ggcgccggca
     3961 acgacagcgc cgccgaacac cgcacggcca ccgtcgccgc ctgggcgctg gcacactcac
     4021 ccttgggcat caccaaagtg gtcgtgggca atcaggaatg gcggcccgac cgcgacggct
     4081 ggcatccggc cagtgccagt accaccgccg ccgtcggtac cgtcgtgatg acggtcgctg
     4141 cccgctagcg cctgtcgcag ccgaagggac gccatgccga acaccgacgt tcgagccgtg
     4201 caaccaagcg ctgacgcgat gctcgccggc gccacgagcc gcgaaccgat gaagacggct
     4261 gactcgctgt cgagcgccag cttcgaacga gtcgtgctcg acggcgatcg attcgtcgtg
     4321 aagcatctct cgacgtcgag cgactggatc atgcgcgcga gcggcgacgt cgcctgccga
     4381 ccgctgtgga tgtggcgaag cggaacgctc gacaccttgc ccgactgcct cgacccgctc
     4441 atcgtcgaca tcgcccacga ctcgaccacg ggcgtcacca cactgctcat gccggacgtc
     4501 gaggagttgc tcgtccccga aggcgccggc cgtctcgagg tcgaacagca acatcggttc
     4561 ctcgaccaca tggcagcgct gcatgccgcg ttctggaact gggacggtcc acccgcgctg
     4621 acacccatgg caaaccgcta cacgacgctg actcctatga ccagcctcgt cgaggctgag
     4681 ctcggcaata ccgacggcgt tccctcttac ctggagggcg cgtggcgtcg gctcatcgag
     4741 gtgtcccccg agatcggcga gcttgccctc gccatcaccg ccgacccagg accactcgtc
     4801 gacgccctcg accaaacgcc gtccaccctg attcacggcg actggaaggc cggcaacctc
     4861 ggcagccacc tcgacgggcg gaccattctg atcgactggc aatggccggg gcaggcggcc
     4921 ggctgcgtcg acctcggctg gtatctggcg gtgaactgcg accggctgcc cgaatcgaag
     4981 gaagcgacca tcgccgcgta tcgcgacgcg ctcgtgcgtc acggcgtcga cgtgggcgat
     5041 tggttcgatc ggcaactcga gctcgcgctg ctcggtgcct tcgtgcaact cggctggtcg
     5101 aagactggcg accccgcgga actgaaatgg tgggtcgatg cggtgctgcc gaccgctcgt
     5161 gagttgctgc ggtgaccgat cccgctgacg cggctttcga cgtccgggct gatgttcggg
     5221 ctgcttatga cgcgaccggc gcagcatggg acaacggccc gcgccgcatc tacgaccaac
     5281 tcgccatcgc gttactcgac cgctgcccgg ttccgttcga ccacgcgaga gccgtcgatg
     5341 caggcgccgg aactggtgcg gcgacgaacg aactcgttcg tcgaggcgcg agtgtgacgg
     5401 cggtcgacct gtctgcgggc atgttgcgct gcctcgaccc gaagaacgcg agcttggcgg
     5461 tcggtgacgt cgtcgcgctg ccgattcgca ccgatgcagt cgacatcgcg atggccgcgt
     5521 tcgtgctcaa ccacctgccg cagccgatcg acgcgttgcg cgaactggct cgcatcacgc
     5581 acccggccgg cgccgtgctg gtgtcggtct tcggcgaagc gccgccgcat ccgtcgaagg
     5641 acgcgatcga cggggtggct cgcctgtatg gcttcacggc tcccgcctgg caccggcgac
     5701 tgaaggaaga gttcgaggcg cagacctcga cacctgaacc actcgccacc ctcgcccgac
     5761 aggcaggttt ggtcgacgtg agcgctcggg tcgagcacat tcaaccgacg atgaccgacg
     5821 acgacctcgt cggctggcgc accggcatgg cgcacctcgc gccgttcatc gcgaccctgc
     5881 ccaccgcgac tgcgcaagca cttgcggacg acgcacgcgc agccgtggtc ggcatgccgc
     5941 cgctcgacat cgcgatggtg accctcgtcg cccgccctct tgcgtgatca gccggcggtc
     6001 ggcttcggcg gcgcatcgtc gaacgaagcg cgagagttgt cagtcctacg acgcgataga
     6061 tagcgtcctg ggactgacaa cttcgccgtt cagcgcccgg ctgaggtcaa ccgctgtgct
     6121 gccgccacga cgcgctcgtc ggtcgcggtc agggcgatgc gcacatg
//
LOCUS       DASWVF010000835         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_24040, whole genome shotgun sequence.
ACCESSION   DASWVF010000835 DASWVF010000000
VERSION     DASWVF010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24040"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1310
                     /locus_tag="VGD55_14805"
     CDS             66..1310
                     /locus_tag="VGD55_14805"
                     /inference="COORDINATES: protein motif:HMM:NF037721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871666.1"
                     /translation="MRRRLLISTLGVAVVSVILLGIPLGIVTTRLIRAEAQKRINAEA
                     AQVAAAVNGETDTSVAGTGDVLGQFVGEDRFISVRRNGLLVSTGTTIKGPVERGVALG
                     AHGEAVTVVESANSTNDELASVWFGVAGAAVVALAAAVELARRQAKRLAGPLEDLAVG
                     AEQLGAGDPRPRNHRYGVPELDRVAEVLDRSAEEIGRLLGAERALAAEASHQLRSPLT
                     ALSIRLEEIVATSTEPAIREDATAALVQVERLAAVVDSLLAEHGRSARTASGVLVDVD
                     AVLGQQAVEWEPAFRRAHRKLVREGSPGLKVFATPGGLAQVVATLIDNALVHGAGTVT
                     VNTRHASGHIVLEVHDEGPGVPDELVPRIFERSFSGAQRTGLGLALARDLVSADGGRL
                     ELAQPRPPIFAIFLPPTAPDRA"
     gene            complement(1307..1795)
                     /locus_tag="VGD55_14810"
     CDS             complement(1307..1795)
                     /locus_tag="VGD55_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0871667.1"
                     /translation="MNVVRSLYGRVAHLRHEVAKFGSVGLVAFVIDAGLFNYLVTPEG
                     SLHKHPLTAKAVSTAVAATFAYFANRHWTWRHRSRTGLGREYVLFFVVNLIGLGIADS
                     CLAISEYGLHQHSSLAKNISANVIGLVLATSFRWWAYRRWVFIDVDEPERSEEAAEAS
                     VV"
     gene            1952..>3055
                     /locus_tag="VGD55_14815"
     CDS             1952..>3055
                     /locus_tag="VGD55_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0871668.1"
                     /translation="MAAGVSGTSEFDYPLDDEHGALRSTVQDFARQVVAPRAAAIDEA
                     HEFPYDIVAGMAQMGLFGLPFPQEYGGMGGDYFALCLAIEELARVDSSVAITLEAAVS
                     LGAMPVFRFGNEEQRSTWLPQLASGEALAAFGLTEPDGGTDAGATRTRAVLADGVWRI
                     DGTKAFITNAGTDITRLITVTAVTGEVAGRKEISSLLVPVPCEGLSTAASYSKVGWHA
                     SDTREVSLVGVAVPEANLLGEQGRGFANFLSILDEGRIAIAALAVGLAQGCVDESIAY
                     ATQRKAFGSAIGAFQAIQFKIADMAVRTHTARLAYHDAAARMLAGKPFKKQAAMAKLY
                     ASEIAVTNAREATQIHGGYGFMNESPVARFWRDS"
BASE COUNT          518 a          994 c         1052 g          491 t
ORIGIN      
        1 gagttgggtt gaacccgtcg cgctgacctt gtcagtgcgg gcggccccga cgccgaggag
       61 gacgggtgcg taggcggctc ctgatctcca ccttgggggt cgctgtcgtt tcggtgatcc
      121 tgctcgggat ccccctcggc atcgtcacga cgcggctgat ccgtgccgaa gctcagaagc
      181 ggatcaacgc ggaggcagcc caggtcgccg ccgctgtcaa cggtgagacg gacacctcgg
      241 tcgcaggcac cggcgacgtg ctcgggcagt tcgtcggcga agaccgcttc atctccgttc
      301 gccgcaacgg cttgctggtg tcgacgggca ccacgatcaa aggccccgtc gagaggggcg
      361 tcgcactcgg cgcgcacggc gaggccgtca cggtcgtcga gtcggcgaac tcgaccaacg
      421 acgagttggc cagtgtctgg ttcggagtgg ccggagccgc cgtcgtcgcg ctcgccgcag
      481 cggtcgaact ggcgcggcgg caggccaagc ggctcgcggg accgctcgag gacctcgccg
      541 tcggagccga acaactcggt gcgggcgacc cgcgccctcg caaccaccgg tacggcgtgc
      601 ccgagctaga cagagtggcc gaggtgctcg atcgcagcgc cgaagagatc ggccgcctgc
      661 ttggtgccga gcgcgccctg gccgccgaag cgtcacacca gttgcgcagc ccgttgaccg
      721 cgttgtcgat ccggcttgaa gagatcgtcg cgacgtcgac ggagcctgcg attcgcgagg
      781 acgcgacggc ggcgctggtg caagtcgagc ggctcgccgc cgtcgtcgac tcacttctcg
      841 ccgagcacgg ccgcagcgcg cgcacggcgt caggcgtgct cgtcgacgtc gacgcggtgc
      901 ttggtcagca ggcggtcgag tgggagccgg cgtttcgtcg ggcgcatcgc aaactcgtgc
      961 gcgaggggag tccaggcttg aaggtgttcg cgacacctgg cggcctcgcg caggtcgtgg
     1021 ccacgctgat cgacaacgcg ctcgtacatg gcgcgggcac tgtcaccgtg aacacccggc
     1081 atgcgagcgg gcacatcgtg ctcgaggtcc acgacgaagg ccccggcgtg cccgacgagc
     1141 tcgtcccacg catcttcgaa cgatcgttca gtggagccca gagaaccgga cttgggctcg
     1201 cactcgcccg tgatctcgtg tcagccgacg gcggccgcct cgaactcgcg caacctcgcc
     1261 cgccgatatt cgcgatcttc ctgccgccga ccgcaccgga cagggcctag acgaccgacg
     1321 cctcggccgc ttcttcgctg cgctcaggtt cgtcgacatc gatgaacacc caccgtcggt
     1381 aagcccacca gcggaacgac gtcgcgagca cgaggccgat cacgttggcc gagatgttct
     1441 tcgccaggct cgagtgctga tgcaggccgt actcagagat cgcgaggcaa gagtcggcga
     1501 tgccgaggcc gatgaggttc acgacgaaga aaaggacgta ctcgcggcca aggccggtgc
     1561 ggctgcgatg acgccaagtc caatgccggt tggcgaagta cgcgaacgtg gcagctactg
     1621 cggtcgagac ggccttcgcc gtgagggggt gcttgtgcag cgagccctcg ggcgtgacca
     1681 ggtagttgaa gaggcccgcg tcgatgacga acgcgaccaa cccgacgctg ccgaactttg
     1741 cgacctcgtg gcgtagatgc gcgacgcgcc cgtagagaga tcgcacgacg ttcacgtcac
     1801 cacctcgggc tggggcgcac gatgcgcggc ctgaccattc cgactgcgtg ccccatcgag
     1861 gatatcgggt gaaaatggga catgctgagg gaactggccg atctgggcgc ccggcgccac
     1921 cgcgccgtcc gaaaaggaag aggatgtctg catggctgcg ggtgtcagtg gaacctccga
     1981 gttcgactac ccattggacg acgaacatgg ggcgttgcgt tccacggtgc aggatttcgc
     2041 ccgccaggta gtggctcccc gcgcggccgc catcgacgag gcgcacgagt ttccatacga
     2101 catcgtcgca gggatggccc agatgggatt gttcgggctg ccgttcccgc aggagtacgg
     2161 cgggatgggc ggcgactact tcgcgctgtg cctcgcgatc gaagaactcg cgcgcgtcga
     2221 ctcctccgtc gcgattactc tcgaggcggc ggtgtcactc ggcgccatgc cggtgttcag
     2281 attcggcaac gaagagcagc gcagtacatg gctaccgcaa ctcgcgagcg gtgaggcatt
     2341 ggcggcattc ggtctcaccg aacctgatgg tggaaccgat gcgggtgcga cccgcacccg
     2401 agcagttctc gccgacgggg tgtggcgcat cgacgggacg aaggcgttca tcaccaacgc
     2461 aggcaccgac atcacacgac tgatcaccgt caccgccgtc accggcgaag tcgccgggcg
     2521 caaggagatc tcctccctgc tggtgccggt gccatgcgag ggtctgagca ccgcggcgtc
     2581 ctattcaaaa gttggttggc acgcctcgga cacgcgagaa gtctcactcg ttggcgtcgc
     2641 cgtgcccgag gcgaatctgc tcggtgagca gggccggggt ttcgccaact tcctgtcgat
     2701 tctcgacgag gggcggatcg cgatcgcggc gcttgccgtc ggtctcgcgc agggttgtgt
     2761 cgacgaatcg atcgcgtacg cgacgcaacg caaggcgttc ggttctgcga tcggtgcgtt
     2821 ccaggcgatt cagttcaaga tcgccgatat ggcagtgcgc acgcacaccg cgcgcctcgc
     2881 gtatcacgac gccgccgccc ggatgctcgc cggcaaaccg ttcaagaagc aggcggcgat
     2941 ggccaagctg tacgcgtccg agatcgccgt gacgaacgct cgcgaggcga ctcagatcca
     3001 cggcggctac ggcttcatga acgagtcgcc ggtggccaga ttctggcgcg actcg
//
LOCUS       DASWVF010000836         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_508098, whole genome shotgun sequence.
ACCESSION   DASWVF010000836 DASWVF010000000
VERSION     DASWVF010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508098"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..998
                     /locus_tag="VGD55_14820"
     CDS             54..998
                     /locus_tag="VGD55_14820"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0871669.1"
                     /translation="MSEVRVQVWGDGAALPSELGRTVVTIGVFDGVHRGHQRIVYRAL
                     ARAAELRLPAVAVTFDPMPEYVLRPDTKPPMLTTLERRLDLLGELGLDGAYVINFTDE
                     FSTRSPGEFVQSVLVDKLRAVDVVVGANFRFGRRAAGDVATLRDFGRDAGFMADGIEL
                     AGPGTGSPVYSSSWIRDRIAAGDVESAAEALGRPHRLEGRVVHGDHRGRELGYPTANL
                     ELPSALAVPADGVYAGLLDGLPAAVSVGTNPTFAGTSRRVEAYVIDEVDLDLYGRVMA
                     LDLVRRLRPMVRFDGIEALVAQMAVDVEESRRFLSGKS"
     gene            1129..1398
                     /gene="rpsO"
                     /locus_tag="VGD55_14825"
     CDS             1129..1398
                     /gene="rpsO"
                     /locus_tag="VGD55_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0871670.1"
                     /translation="MALDTATKNSILAEYGTSDGDTGSPEAQVAMLSKRIADLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLLKYLANKDITRYRTLIERLGLRR"
     gene            1651..3867
                     /locus_tag="VGD55_14830"
     CDS             1651..3867
                     /locus_tag="VGD55_14830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0871671.1"
                     /translation="MADHEITTADAVIDNGKFGTRTIRFETGNLARQAAGSAVVKFGD
                     SVLLSATTASKQPKDQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSEDAILTCRLT
                     DRPLRPSFVKGLRNEVQVVITVLALDPDHLYDVIAINAASMSTQLAGLPFSGPIGAVR
                     VAHIEGQWVAFPYHSELERATFDMVVAGRVLPDGDVAIMMVEAEATAHTFGLVRDGAT
                     APTEEVVAQGLEASKAFIKTLCAAQLEVAAGAAKPTADFPVFLEYQDDVFAAVEAAVS
                     TELAQAMTIISKAEREDELDRVKAVAVEKVGSRFEGREKEIGGAFRSLTKKLVRQRVI
                     RDKVRIDGRGLTDIRQLSAEINVLPRVHGSALFERGETQILGVTTLNMLRMEQMLDTV
                     SPETRKRYMHNYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVLPTREDFPYAIR
                     QVSEALGSNGSTSMGSVCASTMALLAAGVPLRAPVAGIAMGLISDTVDGKTQYVTLTD
                     ILGAEDAYGDMDFKVAGTSEFVTALQLDTKLDGIPASVLAGALTQAREARFTILDVMA
                     KAISEPAEMSQYAPRIITIKIPVDQIGAVIGPKGKMINQIQDETGAEITIEDDGTIYI
                     GATDGPKAEAARAQINAIANPTMPEVGERYLGTVVKTTAFGAFISLVPGKDGLLHISE
                     LRKISGGKRVENVDDVVKVGDKVQVEIKEIDPRGKLSLVPVVEGEAASSGSVSTNE"
BASE COUNT          688 a         1284 c         1291 g          675 t
ORIGIN      
        1 gaaaggagcg cgatcgcggc tggtagatac tgcgggggcc acgcgaggtg acggtgtcgg
       61 aggtgcgggt gcaggtctgg ggagacggcg ccgccttgcc ctcggagctc ggacgcacgg
      121 tcgtcacgat cggggtgttc gacggtgtcc atcgcggcca ccagcgcatc gtgtatcggg
      181 cacttgcccg ggccgcagag ctgcggcttc ccgccgtcgc ggtcaccttc gatccgatgc
      241 ccgagtacgt cctgcgccct gacacgaagc caccgatgct gacgacgctc gaacgccggc
      301 tcgatttgct gggcgagctc ggcctcgacg gcgcgtacgt catcaacttc accgacgagt
      361 tctccacccg gtcgccaggc gaattcgtgc agtcggtgct cgtcgacaag ctgcgcgccg
      421 ttgacgtggt cgtcggtgca aacttccgat tcggacgccg agcggcgggc gatgtcgcta
      481 ccctgcgcga ttttgggcgt gacgccgggt tcatggccga cggcatcgag ctcgccggcc
      541 ccggtactgg ctcgccggtt tactcctcga gctggatcag ggacaggatc gcggccggtg
      601 acgtcgaatc ggcggccgag gcacttgggc gtccgcaccg gctcgaaggc cgcgtcgtcc
      661 acggcgacca ccgcggtcgt gagctcggtt acccgacggc caatctcgag ctgccgtccg
      721 ccctcgccgt ccctgccgat ggcgtgtacg ccggactgct cgatggcttg ccggcggcag
      781 tctcggtggg cacgaacccc acctttgctg gcactagccg gcgggtcgag gcgtacgtga
      841 tcgacgaggt cgaccttgac ctctatgggc gggtcatggc ccttgacctg gtacgacgcc
      901 tgcgcccgat ggtgaggttc gacgggatcg aggctctggt cgcccagatg gcggtcgatg
      961 tcgaggagag ccgcaggttt ttgagcggca aatcttagag ccgcggcttt tttgagcggt
     1021 aaatcctagg gtggtaacct agcgataagg ccggtcacgt accggcgtgt gtttccctgc
     1081 cctcgtgaat cggaccgagg gcacacacga aagcaacgaa ggagtcctgt ggcgctcgac
     1141 accgcgacca agaattcgat cctcgccgaa tacggcacca gcgacggcga caccggatcc
     1201 ccggaggccc aggtcgcgat gctttcgaag cggatcgccg atctgaccga gcacctcaag
     1261 gtccacaagc acgatcacca cagccgtcgc ggcctgctgc tgcttgttgg ccgccgccgc
     1321 cgcctcctca agtacctcgc aaacaaagac atcacccgct accgcacgct catcgagcgt
     1381 ctcgggctgc gtcgatagcg ggaatgggcg cggtccaagc tctcgggccg accgctcccc
     1441 gctgtaaacg cgtgcgactg aaccccagcc gcatgcgcgc tctcaccatc atgggagccc
     1501 gaacgagcgc cgcctggccg gtcctcggta gtggtccccg ggaaagttcg ccgccgcgat
     1561 ttcatcgcag ccgcggcccg gggacttcga tcgaagaccg gcggagattg ccgggctcgg
     1621 cgggttccac gacaaccctt tggagggttc gtggctgacc acgagatcac taccgccgac
     1681 gccgttatag ataacggcaa gttcggcacc cgcacgatcc gtttcgagac cggcaatctg
     1741 gctcgccagg ccgccggttc agctgtcgtc aagttcggcg acagcgtgct gctttcggcg
     1801 accaccgcga gcaagcagcc caaggaccag ttcgacttct tccccctgac ggtcgacgtc
     1861 gaagagcgga tgtacgccgc gggccgcatc ccgggctcgt tcttccgtcg cgaaggccgc
     1921 ccgagcgagg acgcgatcct cacctgccgg ctgaccgacc ggccgctgcg cccgagcttc
     1981 gtcaagggcc tgcgcaatga ggtccaggtc gtcatcaccg tgctggctct cgaccccgat
     2041 cacctctacg acgtgatcgc gatcaacgcc gcatcaatgt cgacccagct cgcaggcctg
     2101 ccgttcagcg gcccgatcgg tgcggtgcgt gttgctcaca tcgagggcca gtgggtggcg
     2161 ttcccgtatc actccgaact cgagcgtgcg acgttcgaca tggtcgtcgc gggccgggtt
     2221 cttcccgacg gcgacgtcgc catcatgatg gtcgaggccg aggccactgc gcacacgttc
     2281 ggcctggtgc gcgacggtgc gaccgccccg accgaagagg ttgttgctca gggtctcgag
     2341 gcctcgaagg ccttcattaa gaccctatgt gcagcacagc tcgaggtcgc tgcgggtgcg
     2401 gcgaagccga ccgccgattt ccccgtcttc ctcgaatatc aggacgacgt atttgcggcc
     2461 gtcgaagctg ccgtcagcac cgagttggcg caggcaatga cgatcatcag caaggccgag
     2521 cgcgaagacg agctcgaccg cgtcaaggct gtcgccgttg agaaggtcgg gtcgcgcttc
     2581 gaaggtcgcg aaaaggagat cggcggcgcg ttccggtcgc tgaccaagaa gctggtacgc
     2641 cagcgggtta tccgcgacaa ggtgcgcatc gacggtcgcg ggcttaccga catccgccag
     2701 ctctctgccg agatcaacgt gttgccgcgg gtgcacggct cggcgctgtt cgagcgcggc
     2761 gagacccaga tcctgggcgt caccacgctg aacatgctgc gcatggagca gatgctcgac
     2821 acggtgtcgc cagagacgcg caagcgctac atgcacaact acaacttccc gccgtactcg
     2881 accggcgaga ccggtcgggt cggttcgccg aagcgccgcg agatcggcca cggcgcgctg
     2941 gccgagcggg cgctcgtgcc ggtgcttccg acgcgcgagg acttccctta cgcgatcagg
     3001 caggtctcag aagcgctggg ttccaacggc tcgacgtcga tgggctcggt gtgtgcatcc
     3061 acgatggcat tgctcgcagc aggcgttccg ttgcgcgcac cggttgccgg catcgcgatg
     3121 ggtctgatct ccgacaccgt cgatggcaag acgcagtacg tcacgctgac cgacattctc
     3181 ggtgcagaag atgcctacgg cgacatggac ttcaaggtcg ccggcacttc tgagttcgtc
     3241 acggcacttc agcttgacac caagctcgac ggcatcccgg cgtcagtcct cgcgggcgct
     3301 ttgactcagg cccgcgaggc tcgtttcacg atcctcgacg tgatggcgaa ggccatcagc
     3361 gagccggccg agatgtcgca atacgcgccg cgcatcatca cgatcaagat ccccgtcgat
     3421 cagatcggcg cagtcatcgg gccgaagggc aagatgatca accagatcca ggacgaaacc
     3481 ggcgccgaga tcacgatcga ggacgacggc acgatctaca tcggtgcgac cgacggcccg
     3541 aaggctgaag cggcccgcgc gcagatcaac gcgatcgcga acccgacgat gccggaggtt
     3601 ggtgagcgct acctcggaac cgtggtgaag acaacggcgt tcggtgcatt catctccctc
     3661 gttcctggca aggacggttt gctgcacatc tccgagctcc gcaagatctc gggcggcaag
     3721 cgggtcgaga acgtcgacga cgtcgtcaag gtcggcgaca aggtgcaggt cgagatcaag
     3781 gagatcgacc cgcgcggcaa gctgtcgctc gttccggtcg tcgaaggcga agctgcttct
     3841 tcgggatcgg tgagcacgaa cgagtgagtc cttatctgcg ggccgaacaa aagccaggta
     3901 gtaccaagct gttgatctcc ggcgctgacg gcgcggtg
//
LOCUS       DASWVF010000837         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_49186, whole genome shotgun sequence.
ACCESSION   DASWVF010000837 DASWVF010000000
VERSION     DASWVF010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49186"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VGD55_14835"
     CDS             complement(<1..632)
                     /locus_tag="VGD55_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0871672.1"
                     /translation="MTVALSACGGVGSSSGSGGPMLVMARVKDAVVLDPSHATDGLSL
                     NTTNEVMQNLVAFKPGSFDVGPDAAQHWSVTPDGKTWTFDLTPGMVFSDGTPLDAAAV
                     KFNFDRWRLPSHPAHGNFTYAYYVDVFGGFPGIITDVKAPTKTRVVFTLKVPRGSFLH
                     DIAEQAFALGSPQAILSDPKAFELKPVGSGPYEVSEWVRDDHSTLVANPK"
     gene            complement(692..>1852)
                     /locus_tag="VGD55_14840"
     CDS             complement(692..>1852)
                     /locus_tag="VGD55_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871673.1"
                     /translation="LGAQVSYDSKAARVEVTSLVVGRTPGLEQHTPGGSTQIVGTVSA
                     VDLNSAPETLTVTKGPSVRTIAITSDAKIIVQDVVTRTATSASLADVHVGDAASIVLR
                     GNGSVDQVVLRYASRAGTIAAVSPSAFVLQSGYIVQPDKSTAITLNGQPATIGDLKVG
                     DSVTVRLNPDTGEKRQILISRAMPAPPTPAGDAQITDFGVAAPGALRAGESFTVTMHG
                     TPGGVATFDIGTYVQGLPVPEDRRQPGTYTTTYKVPEGVNFGRTSIYGHLTVGATTAP
                     RAEAPQLLVVTTTPPQIVDIAPSFGLTVNNNKPSIYATYRTPTDVGISVSSVRLEVNG
                     LDVTPSATRTDSFVTYSPSVPLADGPVSVKVSVADNAGNLVTRSWMFSVRTH"
BASE COUNT          301 a          595 c          634 g          322 t
ORIGIN      
        1 catttcgggt tcgcgaccag cgtgctgtgg tcgtcgcgca cccactcgga gacctcgtac
       61 ggaccgctgc ccaccggctt gagttcgaac gccttagggt cggagaggat cgcctggggc
      121 gagcccagcg caaatgcttg ctcggcgatg tcgtgcagaa acgagccgcg cggaaccttc
      181 agcgtaaaca cgacacgcgt cttcgtcggc gccttcacgt cggtgatgat gccggggaat
      241 ccgccgaaca catccacgta gtacgcgtag gtgaagttgc cgtgcgccgg atgcgagggc
      301 aaacgccagc gatcgaagtt gaatttcacc gcggccgcgt cgagcggcgt cccgtccgag
      361 aacaccatgc ccggcgtgag gtcgaaggtc caggtcttgc cgtccggcgt gaccgaccaa
      421 tgctgcgccg cgtcgggacc gacgtcgaag ctgccgggct tgaacgcgac cagattctgc
      481 atcacttcgt tggttgtgtt gagcgaaagc ccgtcggtcg catgcgaggg atcgaggacg
      541 accgcatcct tcacccgcgc catcaccagc atcggcccgc ccgatccgct gctcgagccg
      601 acgccgccgc acgcgctcag cgcgacggtc aaaacgagcg cgaccagagc gattctcgaa
      661 ggcgtgcgaa agatggcgtt ctcctcgtcg atcagtgggt gcggacgctg aacatccagc
      721 tgcgggtcac caagttgccg gcattgtccg cgacggagac cttgacgctg accggaccat
      781 cggcgagcgg aacgctgggg ctataggtca cgaagctgtc ggtgcgcgtc gccgatggcg
      841 tcacgtcgag accattgact tccagccgta ccgacgagac gctgataccg acgtcggtcg
      901 gcgtgcggta cgtcgcataa atcgacggtt tgttgttgtt gacggtcagg ccgaacgacg
      961 gcgcgatgtc gacgatctgc ggcggcgtcg tggtgaccac cagcagctgc ggtgcctccg
     1021 cgcggggggc ggtcgtcgca ccgacggtca ggtgaccgta gatcgaggtc cggccgaagt
     1081 tcacgccctc aggtacctta tacgtcgtcg tgtaggtgcc gggctggcgg cggtcctccg
     1141 gcacgggcag gccctgcacg taggttccga tgtcgaacgt cgccacgccg cccggcgtcc
     1201 cgtgcatcgt gacggtgaac gactctccgg cgcgcagcgc accgggtgcg gcgacgccga
     1261 agtccgtgat ctgcgcatca cccgccggcg tggggggggc cggcatcgcg cgcgagatca
     1321 agatctggcg cttctcgccg gtgtccggat tgaggcggac ggtcacgctg tcgccgacct
     1381 tcagatcgcc gatcgtcgcc ggctggccgt tgagcgtgat cgcggtactc ttgtcgggct
     1441 gcacgatgta tccgctttgc aggacgaacg cgctcggcga aacggcggcg atcgttccgg
     1501 cgcgcgaggc atagcgtagg acgacctgat cgacgctgcc gtttccgcgc agcacgatgc
     1561 tggccgcgtc gccgacgtga acgtcggcca gcgacgccga cgtcgccgtc cgcgtcacga
     1621 cgtcttgcac gatgatcttg gcgtcggagg taatcgcgat cgtgcgcacc gacgggccct
     1681 tcgtgaccgt cagcgtttcg ggtgcggagt tcaagtcgac cgcgctgacg gtaccgacga
     1741 tctgcgtcga tccgccgggg gtgtgctgtt ccaggcccgg tgtgcggcct acaaccagcg
     1801 aggtcacctc gacccgcgcg gctttactgt cataggagac ttgggcgccc ag
//
LOCUS       DASWVF010000838         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_483019, whole genome shotgun sequence.
ACCESSION   DASWVF010000838 DASWVF010000000
VERSION     DASWVF010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483019"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..410
                     /locus_tag="VGD55_14845"
     CDS             72..410
                     /locus_tag="VGD55_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0871674.1"
                     /translation="MKLITAVVKPFKLDEVKAAILEAGASGMTTSEVRGFGRQRGHTE
                     VYRGAEYVVDLIPKIRIEIVVDDDEVDKIIDAIVASARTGKIGDGKVWSIPVDEIVRV
                     RTEEHGTDAV"
     gene            complement(427..978)
                     /locus_tag="VGD55_14850"
     CDS             complement(427..978)
                     /locus_tag="VGD55_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871675.1"
                     /translation="MTTQIQPDDDSGGYIRGGGGVIDTRRLFRVFVGSAVVALVALVV
                     ALTIEAAHKNSRIDSLQHHGVPVDVTVTKCLGILSGTGITVTTFQCNGSFNLDGRSYN
                     AVIAGSNLNHSPGDVVKAVADPKHPTSISTATSLVNTHSSWRAYTAPAILLVLLLVLI
                     LFAFWWSRRNANARLPEGEARVS"
     gene            complement(978..1997)
                     /locus_tag="VGD55_14855"
     CDS             complement(978..1997)
                     /locus_tag="VGD55_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY0871676.1"
                     /translation="MDFGVVLQCNPPAWRTVDLARQAELAGFGYVWTFDSHLLWQEPY
                     VIYSQILSSTHRVIVGPMVTNPATRDWTVTASTFATLNEMFGNRTVCGIGRGDSAVRV
                     INGKPVTLAELRDSIAVIRSLANGDAADYKDSTLRLTWNPHSRLPIYVAAYGPKALAL
                     TGEIGDGFILQLADLAVAEWSIKAVRDAAAAAGRDPASVKICVAAPAYIGDDIAHQRE
                     QCRWFGGMVGNHVADIVARYGADGGPVPQALTDYIAGREGYDYNEHGRVGNTHADFVP
                     DEIVDRFCVLGTADEHIEKLRALKALGVDQFAVYLQHDAKEATLQAYADKVIPALADT
                     VAAKS"
     gene            complement(2009..>2908)
                     /locus_tag="VGD55_14860"
     CDS             complement(2009..>2908)
                     /locus_tag="VGD55_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0871677.1"
                     /translation="ILRAMQTAAGNGSMVMMHAENGIAIDVLVSQALARGETAPRYHG
                     LTRPWETEEEATHRAIMLARLTGAPLYIVHMSAKQAVATLAAARDDGWNVFGETCPQY
                     LYLSLEEHLSAPGFEGAKYVCSTPLRSRAEGHHAELWKALRTNDLSVVSTDHCPFCMK
                     EQKELGVGNFSKIPNGIGSVEHRMDLIYQGVVDGHITLARWVELCSTTPARMFGLYPR
                     KGVIAPGSDADIVVYDPNATTRISVDTHHMNMDYSAWEGFEVAGRVDTVISRGTVVVR
                     DREYIGSKGHGRYLKRGQSQYLI"
BASE COUNT          542 a          873 c          955 g          538 t
ORIGIN      
        1 gacgacgacc tggcctatga gcaggcgcgg cagacgaagt ccctgcagtt gaagaagagc
       61 ggtagacgcg catgaaactg atcaccgccg tcgtcaagcc tttcaagctc gacgaggtca
      121 aagcggccat ccttgaggcc ggcgcgagcg gtatgacaac gtcggaggtc cgcggcttcg
      181 gccggcagcg cggccacacc gaggtgtacc ggggtgccga gtacgtggtc gacctgatcc
      241 cgaagattcg catcgagatc gtcgtcgacg acgacgaggt cgacaagatc attgacgcga
      301 tcgtcgccag tgcccgtacg ggcaagatcg gtgacggcaa ggtgtggtcg atcccggtcg
      361 atgaaatcgt ccgcgtacgc accgaggagc acggcaccga cgcagtctga tcggcggcat
      421 gtagagctac gaaacgcgcg cctctccttc ggggaggcgc gcgtttgcgt tgcgtcgaga
      481 ccaccagaag gcgaacaaga tgagcaccag gagcaggacc aacaaaatcg ccggtgcggt
      541 gtaggcgcgc cacgacgagt gggtgttcac cagcgaagtc gctgttgaga tggacgtcgg
      601 gtgcttcggg tcggctacag ccttcacgac atcgcctggg gaatggttga ggttcgagcc
      661 cgcaatgact gcgttgtagc ttcggccgtc gaggttgaac gacccgttgc actgaaatgt
      721 cgtgactgtg atgccggtgc cggacaagat gccgagacac ttggtcacgg tgacgtcgac
      781 cgggacgccg tggtgttgca gcgagtcgat ccgtgagttc ttatgcgccg cctcgatcgt
      841 caatgccacc acgagggcaa caagggctac gactgctgag ccgacgaaaa cacggaacaa
      901 tcgacgggta tcgatcacac caccgccgcc gcggatataa cccccgctat cgtcgtccgg
      961 ctgaatctgg gttgtcatca acttttcgcg gccaccgtgt cggctagggc cgggatcacc
     1021 ttgtcggcgt acgcctgaag cgtggcctct ttcgcgtcgt gctggaggta gaccgcgaac
     1081 tgatcgacgc cgagtgcctt cagcgcgcgg agtttctcga tatgttcgtc agcggttcca
     1141 agaacacaga accgatcgac gatctcgtca ggcacgaagt cggcatgcgt gttcccgact
     1201 cggccgtgtt cgttgtagtc gtaaccctcg cgacccgcga tgtagtcggt gagcgcctgc
     1261 ggcactgggc cgccgtccgc cccatatctc gcgacgatgt cggcgacgtg attgccgacc
     1321 atgccgccga accatcggca ttgttcgcgt tgatgcgcga tgtcgtcgcc gatgtaggcg
     1381 ggggcagcga cgcagatctt gacggaagcc gggtcacggc ccgcggccgc ggcggcatcg
     1441 cgcaccgcct tgatgctcca ctcggcaaca gcgaggtcgg cgagttgcaa gatgaagccg
     1501 tcgccaattt caccagtcag ggcaagggct ttgggtccgt acgcagcgac gtaaatgggg
     1561 agtcggctgt gcggattcca ggtcagtcga agggtgctgt ctttgtagtc tgcagcgtca
     1621 ccgttcgcga ggctgcggat cacggcgatc gagtcgcgta gctccgcgag tgtcacgggt
     1681 ttgccgttga tgactcgcac cgcagaatcg ccgcgaccga tgccgcacac ggtgcgattg
     1741 ccgaacatct cattgagagt ggcgaacgtc gatgcggtga cggtccagtc acgggtggcg
     1801 gggttggtga ccatcggtcc cacgatgacg cgatgcgtcg acgacaggat ctggctgtag
     1861 atgacgtagg gctcctgcca gagcaggtga gagtcgaagg tccatacgta gccgaatccg
     1921 gcgagctcgg cctgcctcgc caagtcgacg gttcgccatg ccggcggatt gcactggagc
     1981 acaacgccga agtccatcgt caagctccct agatcaggta ctgcgattgg ccacgcttga
     2041 ggtagcggcc gtgacccttg ctgccgatgt attcacgatc gcggacgacg acggtgccac
     2101 gcgagatgac ggtgtcgacc cgacccgcga cctcaaaacc ctcccacgcc gagtaatcca
     2161 tgttcatgtg atgcgtgtcg acggagatgc gtgtcgttgc attcgggtcg tacacgacga
     2221 tgtcggcgtc ggatcctggc gcgatgacac ccttgcgcgg gtagagcccg aacatgcgcg
     2281 cgggtgtggt cgagcacagt tctacccagc gcgcgagggt gatgtgcccg tcgacgacgc
     2341 cctggtagat caggtccatg cggtgctcga ctgacccgat gccgttcggg atcttcgaga
     2401 agttgccgac gcccagctcc ttttgttctt tcatacagaa ggggcagtgg tcggtgctca
     2461 cgaccgacag atcgttggta cgcagcgctt tccacaactc ggcgtgatga ccttccgcgc
     2521 gtgaacgcaa cggcgtcgag cagacgtact ttgcgccctc gaaccccggc gccgacagat
     2581 gctcttcgag ggagaggtag agatattgag ggcacgtctc accgaacacg ttccacccgt
     2641 cgtcgcgagc cgccgccagc gttgcgacgg cctgtttcgc cgacatgtgc acgatgtaga
     2701 gcggggcacc ggtaaggcgg gccagcatga tcgcgcggtg cgtcgcctct tcctcggtct
     2761 cccacggccg ggtgaggccg tggtagcgcg gcgccgtctc accgcgcgcc aacgcctgcg
     2821 acaccaggac gtcaatcgcg atgccgttct ctgcgtgcat catgaccatc gacccgttgc
     2881 ctgcggcggt ttgcatcgcc cgaaggat
//
LOCUS       DASWVF010000839         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_431631, whole genome shotgun sequence.
ACCESSION   DASWVF010000839 DASWVF010000000
VERSION     DASWVF010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431631"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGD55_14865"
     CDS             <1..975
                     /locus_tag="VGD55_14865"
                     /inference="COORDINATES: protein motif:HMM:NF039440.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HEY0871678.1"
                     /translation="DRNLEAVVIKVVWPAFVAALEATRYDNSMFVPISFVDFTPGYDT
                     NSAVLFPETVAVREVPKYTWGAIFCDREAARFRRVTAAARETLKLSLPPAAERLVENQ
                     ELAQQTFVLWDLIHDRTHSHGDLPFDPFMIKQRMPYWMYSLEELRCDLQAFRQAVELE
                     AERVTPYGEYVQYAVLFDRLFRFPITGDRVRNYDGLGGQLLFAYLHKNGVLHWTDNTL
                     SIKWGRVAAAVTDLGREVETLYRDGIDRSRVGHWLAGHEFVARYVAPSPLSVWAQGAA
                     ALPLDGAPKGLVDLVLSDEFPLNVFYEALRRKLGDVIESTRGITGVTA"
     gene            972..1679
                     /locus_tag="VGD55_14870"
     CDS             972..1679
                     /locus_tag="VGD55_14870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0871679.1"
                     /translation="MSLDGAVVVVAGAGGGAGAAVVRRLSSAGAIVVTADRSLDSTES
                     LVREIGEAGGQIEAYAVDLLDSSTTHAWADSVTARFGHVDGVVHLVGGWRGGKGITEA
                     DLADWDWLSGLLVRTVQNTTRGFHDQLKASPIGRFVLVSAAEASRPTAKNASYAAAKA
                     AAEAWTLAVADSFRGSEAAATIIVVKALVTPQMREAKPDSAFKGYTDVIDLADTITGL
                     WAKPAEEVNAERVWLTT"
     gene            1664..2731
                     /locus_tag="VGD55_14875"
     CDS             1664..2731
                     /locus_tag="VGD55_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY0871680.1"
                     /translation="MADDVNGQHHDPARRGFASDNYAGVHPEILSALALANGGHVGSY
                     GGDPYTAAAVEVLRRHFGADAEVFFVFNGTGANVVSLQAMTKRWDAVICAESAHINVD
                     ECGAAEKIAGLKLLSVGTPDGKLTPELIDVHAWGFGDVHHAQPRVVSITQSTELGTVY
                     TPSEIAAICEHAHSFGMLVHIDGSRIANAAAALGLPLRAFTTDVGVDVVSLGGTKNGL
                     LYGEGIAVLNPEAVSGVEFLRKAGMQLSSKMRFIGVQFEVLFGTDLWLRSATHANAMA
                     RRLADATADIPGVQLTRQPEANSVYAILPADVTTRLQEQVFFYPWDRATGEVRWVCAF
                     DTTEADVDDFAEAVRKEMTNR"
BASE COUNT          483 a          896 c          956 g          516 t
ORIGIN      
        1 gaccgcaatc tcgaggccgt cgtcatcaag gtggtgtggc cagctttcgt cgccgccctg
       61 gaagcgacgc gttacgacaa ctcgatgttc gttccgatct ccttcgttga cttcacgccg
      121 ggttacgaca cgaactccgc agtgctgttc cccgagaccg tggctgttcg cgaggtgccc
      181 aaatacacct ggggtgcgat cttctgcgac cgtgaggctg cccgcttccg ccgcgtgacg
      241 gcggctgcgc gagagacgtt gaagctctcc ctgccgcccg ctgccgagcg cctcgtcgag
      301 aatcaagaac tcgctcagca aacgttcgtg ctgtgggatc tgattcacga ccgcacccac
      361 agtcacggtg acctgccgtt cgatccgttc atgatcaagc agcggatgcc gtactggatg
      421 tattcactcg aggaactgcg atgcgacctc caagccttcc gccaggcggt cgagctcgag
      481 gccgagcgag tgacccccta cggcgagtac gtgcagtacg cagtcttgtt cgaccggttg
      541 ttccgcttcc ccatcaccgg tgaccgggta cgcaactacg acgggctcgg cggccagttg
      601 ctgttcgcct acctgcacaa gaacggcgtg ctgcactgga ccgacaacac gctgtcgatc
      661 aaatggggtc gcgttgcggc ggcagtcacc gacctcggac gcgaagtcga gacgttgtac
      721 cgcgacggca tcgaccggtc tcgtgtcggg cattggctcg cgggccacga attcgtcgcg
      781 cgttacgtag cacccagtcc gctgtcggtg tgggcgcagg gagcggccgc gttgccgctc
      841 gacggcgcac cgaaagggct cgtcgacctc gtgctgtccg atgagttccc gctgaacgtg
      901 ttctacgagg cattgcgacg caaactcggt gacgtgatcg agtcgacccg cggcatcacg
      961 ggagtgacgg catgagcctt gatggtgctg ttgtcgtcgt cgccggagct ggcggtgggg
     1021 caggcgccgc cgtcgtccgt cggttgtcgt cggcgggcgc gatcgtcgtg actgcggatc
     1081 gctcactcga ctcgacggaa tcgctggtgc gcgagatcgg cgaggccggc gggcagatcg
     1141 aggcctacgc ggtcgacctg ctcgacagct cgacgacgca cgcgtgggcc gactctgtca
     1201 ccgcgaggtt cggccacgtc gacggcgtag tgcatctcgt cggcggctgg cgaggcggca
     1261 agggcatcac ggaggccgac ctcgccgact gggactggtt gtcaggcctg ctcgtgcgca
     1321 cggtgcagaa cacgacccgc gggttccacg accagttgaa ggccagcccg atcggccggt
     1381 tcgtgctggt gtcggcggcg gaggcgtcgc ggccgaccgc gaaaaacgct tcctacgccg
     1441 cggcgaaagc agccgcagaa gcctggacac tcgccgtcgc cgattccttc cgcggctcgg
     1501 aggctgccgc cacgatcatc gtggtcaagg cgctggtcac cccacagatg cgcgaagcca
     1561 agcccgactc ggccttcaag ggctacaccg atgtcatcga tctagctgac acgatcactg
     1621 gcctgtgggc caagccggcg gaagaagtaa acgcggaacg ggtatggctg acgacgtgaa
     1681 cgggcagcac cacgacccgg ctcggcgcgg cttcgcgagc gacaattacg cgggcgttca
     1741 cccggaaatc ctctctgcgt tagcgcttgc caacggcggg cacgtcggca gctacggcgg
     1801 cgacccctac acggccgccg cggttgaggt gctgcgacga cactttgggg cggacgccga
     1861 ggtgttcttc gtcttcaacg gcaccggcgc caatgtcgtg tcattgcaag caatgacgaa
     1921 gcgctgggac gccgtgatct gcgcggaatc tgcccatatc aatgtcgacg agtgcggtgc
     1981 ggccgaaaag atcgccgggc tcaagcttct ttcggtgggc actcccgacg ggaaactcac
     2041 gcccgagctg atcgacgtcc atgcctgggg tttcggcgac gtgcaccacg cacagccccg
     2101 cgtcgtgtcg atcacccaga gcactgagct cggcacggtc tacacgcctt cggagatcgc
     2161 ggcgatttgc gagcacgcgc attcgttcgg gatgttggtg catatcgacg gctcgcgcat
     2221 tgcgaatgcg gcagccgcct tgggccttcc gctgcgtgca ttcacgaccg acgtcggcgt
     2281 cgacgtcgtc agtctcggtg ggacgaagaa cggcctgctc tacggcgaag ggatcgcggt
     2341 gctcaacccc gaggctgttt ctggggtcga gttcttgcgc aaggcgggca tgcagttgtc
     2401 gtcgaagatg cggttcatcg gcgtgcagtt cgaggtgctg tttggcaccg acctgtggtt
     2461 gcgcagcgcc acgcatgcga acgcgatggc gcgtcggttg gcagatgcga ccgcggacat
     2521 ccctggcgtg caactcaccc gccaacctga agctaactcg gtctacgcga tcctgccggc
     2581 cgacgtgaca acgcgcctgc aggagcaggt tttcttctac ccctgggatc gcgctaccgg
     2641 tgaggtgcgc tgggtgtgcg cgttcgacac caccgaagcc gatgtcgacg acttcgctga
     2701 ggccgtccgc aaagaaatga ccaaccgcta gctcgaaggc ttagtggttg cggaggaccg
     2761 ccgggaatgg gggtgggtgc ccggtcgttg ggcgccggta tgcagatagc cgtgattggg
     2821 acagggaaga tcggcggcac tctcggagag a
//
LOCUS       DASWVF010000840         3924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_406446, whole genome shotgun sequence.
ACCESSION   DASWVF010000840 DASWVF010000000
VERSION     DASWVF010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406446"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1094
                     /locus_tag="VGD55_14880"
     CDS             231..1094
                     /locus_tag="VGD55_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY0871681.1"
                     /translation="MPPRKPSPVGVHVPVAGGLSNGGLAYARDVGAEAIQVFLSNPRG
                     WARNAGDARQDEDFLSRCAEAKMPTFVHAAYLVNLGSPTPATLENSVLSLRHALLRAG
                     AIGARGVVVHAGSAVTLSSKPEAMAQVRENLLPLLDEIPDNGPDVLIEATAGAGQSLA
                     ATVDDLGAYMTALDHHPKLGICLDTCHAFAAGHDLAEPGGVRSTLNALVRAVGRGRLK
                     LLHANDSKDACGSNRDNHERIGKGRIGVEPFAELFRHPATRGVPLVLETPGRAPEHRE
                     DLTLMKVLRDR"
     gene            1152..1931
                     /locus_tag="VGD55_14885"
     CDS             1152..1931
                     /locus_tag="VGD55_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871682.1"
                     /translation="MTQPPYPPPGPPSGYQPGYPQAQPAFTSQLSFGSQPPPRRGLFW
                     SWIAFLALGVVLFAIGVGLAVPWALAASRIGGPPNSTATYGTVTAVQGSTLQVHVLSG
                     TALIERTETHTFNQTSSRTSMMHASDAFDATVTGAPPTSIGDTVSFTYDIDPTQGTYL
                     AVSPSRIKQAGTVPVPMVACFVIGFILGLIGVVLLVTWLVKRHRGKSGGASRYTPPPP
                     GYGPPGYQPPQGYGPPQGYGPPQGYGPPPGYPQQPGYPPQL"
     gene            complement(1944..2141)
                     /locus_tag="VGD55_14890"
     CDS             complement(1944..2141)
                     /locus_tag="VGD55_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871683.1"
                     /translation="MPEKRDAFGPLRPWVAPALGIGLIAAGIAMAAGMAKLYAPIVAA
                     GGAAAVLLVGKLWGTWRGTQS"
     gene            complement(2166..>3924)
                     /locus_tag="VGD55_14895"
     CDS             complement(2166..>3924)
                     /locus_tag="VGD55_14895"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0871684.1"
                     /translation="YRGQSWISTTPTPVRSVPGTTVAVVDTLNVPPVAVTTTTATASP
                     NVATVGQPITFTATVTASDGSIPTGSVDFATSLNQFGQAQLGPDGTASVTTLAVPIGT
                     WNVYATYHPAGLYTSSTGPTALTVGPATSGGGGGSGGGSGGGGGGSVATPITVTVPAG
                     GSASSDPAGTTPDASNPLVVGVTSPSGGTVTIDKTPPDTAVAHYTVLGVGATITAPAA
                     SAANPLTLTFQVFDKQLPTGSQASDLTIFRDGVTVFACTGAGATPDPCVASAVTSGGV
                     STIMIRSSHASTWDVEAAHVGREAGANRLATAVAVSQDSFPNGNAGAVVLARADDYPD
                     ALVGGPLAASKNAPLLLTQGPTLPSVTAAELARVLPSGRTVYVLGGTSAVPASIVAQL
                     TTLGYSVVRYSGADRYATAVAVAGALGNPSTVLLATGTNFPDALAAGPAAAHVHGAIL
                     LTSGSTLPAETSTYLTGAHTVYAIGGPAATAMPSATAIVGTDRFATAAAVATKFFPSS
                     AVAGVATGTGFPDALAGGAQLALTGAPLLLSGSSGLPAATSAYLAADHAALTNVYVYG
                     GTAVLGTGVASQLTASFGS"
BASE COUNT          682 a         1305 c         1276 g          661 t
ORIGIN      
        1 tccgtcgacg tggaccagca tcgcgcaggc gacgaaggcc ctcaccgacg ccgggttcaa
       61 ggtgaacatc ggcaagcgcg gcaggttcgg cgtcgtcatc agccaaagcc ccaagggtgg
      121 cgacatgaag cccgctggca ctgtcatcac gatcaacgcg tactgatctt ccgcatcgtt
      181 ggcgtcagcc cgcgactttc cgaagcggcg aactctgaca tccttggccg atgcctcccc
      241 gcaaaccttc gccagtcggc gtccacgttc cagtggccgg cgggttgtcg aacggggggc
      301 tcgcgtacgc gcgcgatgtc ggtgccgaag ctattcaggt gttcctgtcg aaccctcgcg
      361 gctgggcacg caatgctggt gatgcgcgtc aggacgagga ttttctgtcc cgttgcgctg
      421 aggcgaagat gccgacgttc gtgcacgccg cctatctggt gaacctgggt tcaccgacgc
      481 cagcaacgtt ggagaactcg gtgctgtcgc tgcggcacgc tctccttcgt gcaggcgcga
      541 tcggagcgcg cggtgtcgtc gtccatgcag gttctgcagt gaccctgtcg agcaaacccg
      601 aagccatggc tcaagtgcgg gagaatttgc tgccgttgct ggacgaaatc cccgacaacg
      661 gccccgacgt actcatcgaa gcaactgcgg gcgctggcca atcgttagcc gcaaccgtcg
      721 atgatctcgg cgcgtatatg acggccttgg accatcatcc gaagcttggc atctgcctcg
      781 acacctgcca tgcattcgct gcgggccacg accttgccga accgggtggc gtgcgctcga
      841 cactgaacgc gctcgtgcgg gcagtcggtc gcggcaggct gaagttgttg cacgccaacg
      901 attcgaagga cgcgtgcggc tcgaaccgcg acaatcacga gcgcatcggc aagggccgca
      961 tcggggtcga gccgttcgcc gagctcttcc ggcatccggc cacccgtggg gtgcccctcg
     1021 tgctcgaaac tcctggaagg gcgcccgagc accgcgaaga tctcaccctc atgaaggtct
     1081 tgcgcgatcg gtgagcgcgc gggtttgcat ctcgggcaac cgtcaaggat cttgggctgt
     1141 gtgatgcaaa ggtgacacaa ccgccatatc cgccacccgg gccgccatct gggtatcagc
     1201 ctgggtatcc gcaggcgcag ccggcattca ccagtcagct ctcgttcggc agccaaccgc
     1261 cgccacgcag aggcttgttc tggagctgga ttgcgttcct cgcgcttggt gtcgtcttat
     1321 tcgcgatcgg cgtcgggctc gcggtgccgt gggcgttggc cgcgagccgg atcggcggcc
     1381 cgccgaactc aactgcgacg tacggaacag tgacggccgt ccaaggttcg acgttgcagg
     1441 tgcacgttct gtctgggacg gcgctcatcg aacgcaccga gacgcacacg ttcaaccaga
     1501 caagcagccg gacgtcgatg atgcacgcga gtgacgcatt cgacgcgacg gtgaccggcg
     1561 ccccaccgac ctcgatcggc gacaccgtgt cgttcaccta cgacatcgac cccacgcagg
     1621 gcacgtacct cgcagtttcg ccgtcgagga tcaagcaagc ggggaccgta ccggtgccga
     1681 tggtcgcgtg tttcgtcatc ggcttcatcc tcgggttgat cggtgtcgtg ctgctggtga
     1741 cgtggctcgt caaacggcac cgcggcaagt ccggcggtgc gtctcggtac acgcccccgc
     1801 cgcctggata cggtccgccg ggttaccagc cgccgcaggg gtacggaccg ccgcaggggt
     1861 acggaccgcc gcagggttac ggcccgccac ccggataccc gcagcagccc ggctacccac
     1921 cgcagctcta aacgccggtc acctcacgat tgggtgccgc gccaggtgcc ccacaacttg
     1981 ccgacgagca gcacggcagc tgctcctcca gccgcaacga tgggcgcata cagcttcgcc
     2041 atcccggctg ccatcgcgat tcccgcagcg atcaggccga taccgagcgc cggcgcgacc
     2101 cacgggcgca acggaccgaa cgcatcacgc ttctcaggca tgcgagcacg ctaccggccc
     2161 ggcgttcaac tgccgaacga cgccgtcagt tggctcgcga cgccggtgcc cagcacagcg
     2221 gtgccgccgt acacgtacac gttcgtcaac gcggcgtgat ccgcagctag gtacgcgctc
     2281 gtcgcagctg gtagcccgga cgagcccgag agcaagagcg gcgcacccgt caatgccaac
     2341 tgtgccccgc ccgcaagcgc gtcggggaat ccggtgccgg ttgcgactcc tgcgacagcc
     2401 gaagacggga agaacttagt cgccactgcg gccgccgttg cgaagcgatc ggtaccaaca
     2461 atcgcggtag ccgatggcat cgcggtcgcg gccggaccgc cgatggcata caccgtgtgc
     2521 gcgccggtca ggtacgtcga tgtttctgcc ggcagcgtcg agccgctcgt cagcaggatc
     2581 gcaccgtgaa cgtgcgcggc cgccggcccg gctgccagtg cgtcggggaa attcgtgcct
     2641 gtcgcgagaa gcaccgtgct cggattgccg agcgcacccg cgacagcaac cgccgtcgca
     2701 taccggtctg cacccgagta acgaaccacg ctgtagccga gcgtcgtcaa ctgcgcgacg
     2761 atgctcgccg gtacggctga agtgccgccg agaacgtaga cagtccggcc gctcggcaaa
     2821 acgcgtgcga gttcggcggc tgtcacgctg ggcaaggtcg gaccttgggt gagtaacaat
     2881 ggcgcattct tggacgccgc aagcgggcca cccacaagag cgtccgggta gtcgtcggct
     2941 cttgctagaa cgacagcacc ggcgttgccg ttcgggaagg aatcctgtga aacggccact
     3001 gccgttgcga gtcggttggc gccggcttcg cggcccacgt gcgctgcctc gacgtcccat
     3061 gtactcgcat gcgaactgcg gatcatgatc gtgctcacgc cgccactggt gaccgcggac
     3121 gcaacgcacg gatcgggtgt tgcacccgca cctgtgcaag cgaaaactgt caccccgtca
     3181 cggaaaatcg tcaagtcgga agcctgagat cccgtcggaa gttgcttgtc gaacacctgg
     3241 aacgtcaacg tcagcgggtt ggcggccgac gccgcaggcg ccgtgatggt cgcgccgaca
     3301 ccgagcaccg tgtagtgcgc aaccgctgtg tcgggcggcg tcttgtctat cgtcaccgtt
     3361 ccaccgctcg gactcgtgac gccgacaacg agcgggttcg acgcatcggg tgtcgtgcca
     3421 gccggatcag aggacgccga tccgccggcc ggcactgtca ccgtgatagg agtcgcgaca
     3481 cttccaccgc caccgccacc gctaccgccg ccgctaccac caccgccacc gctagtcgcg
     3541 ggaccgacgg tcagtgccgt ggggcccgtg ctcgacgtgt acaagcccgc cgggtggtac
     3601 gtcgcgtata cattccaggt accgattggc acggcgaggg tggtcaccga cgctgtcccg
     3661 tccgggccga gttgagcctg gccgaattgg ttcaagcttg tggcaaagtc aacggatcct
     3721 gttgggatag agccgtctga tgccgtgact gtggcggtga aagtgatggg ctgcccaaca
     3781 gtcgcgacat ttggtgacgc tgtggctgtg gtcgtcgtga cggcaacggg cggcacgttc
     3841 agcgtgtcaa ctacggcgac cgtggtaccg ggaacgctgc ggacgggtgt cggcgtggtg
     3901 ctgatccacg actgtccgcg gtaa
//
LOCUS       DASWVF010000841         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_380611, whole genome shotgun sequence.
ACCESSION   DASWVF010000841 DASWVF010000000
VERSION     DASWVF010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380611"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGD55_14900"
     CDS             <1..217
                     /locus_tag="VGD55_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871685.1"
                     /translation="GVRLPTVDTIDCVAHKPDVRRAASRWGGGEILEIRGALRRRFWR
                     GAQGAARRCEVEVLAARKISSRSGTPA"
     gene            complement(189..1484)
                     /locus_tag="VGD55_14905"
     CDS             complement(189..1484)
                     /locus_tag="VGD55_14905"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY0871686.1"
                     /translation="MNAPDPSLRATLQLLPFRAVRYDPTVSDLGAVLAPPYDVIDESE
                     QKQLEASDPHNVVRLILPRDPAEPGIVDRYDLAATTFNAWLDAALLAVDHKPALYVYQ
                     QSAAGVVIQRGLLGAVVMQPLDSGVILPHENVRSGPVEDRLSLMRAIGGNPEPIFLLY
                     DGGGATTAISETATAREPLASAITPDGIVHELWAITDGSELDAISADLASRSAMIADG
                     HHRYTTYGYLRDELHAAGKGSGPWDAGLTLLVDDSVATPDIRAIHRVLPGLTLDAAVE
                     AASKAFRVARLETAADDVDGALRLLAQQSGPAFLLSDGRSFTLLCDPDADQLAVARPP
                     HRSMAWWALDASIAGVFVMEHLWGVTDTVGVVEAEHDPAAALHLARDESGIALLLNPA
                     PLAGIFAVARAGEAMPRKSTLFMPKPRSGLVMRAFRFES"
     gene            1625..2074
                     /locus_tag="VGD55_14910"
     CDS             1625..2074
                     /locus_tag="VGD55_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0871687.1"
                     /translation="MDQDQSRDDAQVSDNADRADAAADAADEDAYDWYTRGLRLLQAG
                     SAAAAEQLLDRAAAAEPGSRSVLEALGRAQFEARHYAPAKATFTRIIEADPTDDYGQF
                     ALGLSAARMGELRTAVEHLALAAAMRPDLAHYGRELRMARARLEGAT"
     gene            2071..>2443
                     /locus_tag="VGD55_14915"
     CDS             2071..>2443
                     /locus_tag="VGD55_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871688.1"
                     /translation="MTSAASGDRAQRELLGASQPLATLYDVALLDLDGVVYIGPDPIP
                     GAADALAKARAAGMRLAFVTNNASRDPAAVAAHLVELGVPATADDIVTSSQAAAAVIV
                     ERLGVGATVLVTGSAALRAAVE"
BASE COUNT          375 a          786 c          871 g          411 t
ORIGIN      
        1 cggcgtgcgc ctgccgaccg tcgacacgat cgactgcgtc gcgcacaagc ccgacgtgcg
       61 gcgggcggcg agcaggtggg gtggcgggga gattcttgag atcagaggag cgttgcggcg
      121 ccgattctgg cgcggagcac agggtgcggc gaggcgctgc gaggtcgagg tgcttgctgc
      181 gaggaagatc agctctcgaa gcggaacgcc cgcatgacca gacccgatcg cggcttcggc
      241 atgaacaacg tcgacttgcg gggcatcgcc tcgcccgctc gtgcaacggc gaatatgccg
      301 gcgagcggtg cagggttcag cagcaacgcg atgccactct cgtcgcgcgc caggtgtagc
      361 gccgctgccg gatcgtgttc tgcttcgacc actccgacag tgtcggttac tccccacagg
      421 tgttccatga cgaagacgcc ggcgatgctt gcgtcgagtg cccaccaggc catcgagcga
      481 tgtggcgggc gtgcaactgc gagttgatcc gcgtcggggt cgcacaacaa cgtgaacgag
      541 cggccgtcgc tcagcaggaa cgccgggccg ctctgttgtg ccagcaaacg cagcgcgccg
      601 tcgacatcgt cggcggctgt ttccaaccgc gcgacccgga atgccttcga cgctgcttcg
      661 acggccgcgt cgagtgttag cccgggaagt acgcggtgaa tcgcacggat atccggtgtg
      721 gccacgctgt cgtcgacgag cagcgtgagg ccggcgtccc aagggcccga gcccttgccc
      781 gcagcgtgta gttcgtcacg caagtacccg tacgtcgtgt accggtgatg gccgtcggca
      841 atcatggcgc tccggctggc aaggtcggcc gagatcgcgt cgagctcact gccgtcggtg
      901 atcgcccaca actcgtgcac gatgccgtcc ggggtgatgg ctgacgcgag tggctcgcgc
      961 gcggtcgcgg tctcgctgat cgctgtcgtg gcaccgccgc cgtcgtagag caggaagatc
     1021 ggttccgggt ttcctccgat cgcccgcatg agggaaagcc ggtcttcgac tggcccgctt
     1081 ctcacgttct cgtggggcaa gatcactccg ctgtcgagcg gctgcatgac gacggcgccg
     1141 agcaggccgc gctgaatgac gaccccagcc gcgctctgtt ggtagacgta gagcgccggc
     1201 ttgtggtcga ccgccagcaa tgcggcgtcc agccatgcat tgaaagtcgt cgcggccagg
     1261 tcgtagcgat cgacgattcc gggctcggcc ggatcgcggg ggaggatcag tcgcacgacg
     1321 ttgtggggat cgctcgcctc gagttgcttc tgctcgctct cgtcgatgac gtcatacggc
     1381 ggcgcgagca cggcaccgag gtctgagacg gtcgggtcgt agcgcacagc gcgaaacggc
     1441 agcagttgca gtgtcgcgcg cagggagggg tccggcgcgt tcatggaggt cgtggtttct
     1501 cctttgtaag cgggcggcgc gcggacggcc ctcatgctag tgcgccagac ttggtggaga
     1561 tggacggcgc caacccaggg ctctgccggg ctgcactggc tcatgccggg cggtgaggag
     1621 acggatggac caggatcaat cgcgcgacga cgcgcaggtg agcgacaacg ctgatcgggc
     1681 cgacgcagcc gcagatgccg cggatgagga tgcgtacgac tggtacaccc ggggcctgcg
     1741 tctcctccag gcgggcagtg ccgctgctgc cgagcaactg ctcgaccgcg cggctgccgc
     1801 tgaaccgggt tcccgcagcg tgctcgaggc gctcggacgg gctcagttcg aggcgcgtca
     1861 ctacgcgccg gcgaaggcga ccttcacccg catcatcgag gccgatccga ccgatgacta
     1921 cgggcagttc gcgcttggcc tcagtgccgc tcggatgggc gagctgcgca cggccgtcga
     1981 gcatctcgcg ctggctgctg ccatgcgtcc agatcttgcc cactacggtc gtgaactgcg
     2041 gatggcccgc gcccgactcg aaggagccac gtgacctctg cggcgtcggg tgatcgtgcc
     2101 cagcgcgagt tgctcggagc gagccagccg ctcgcgacct tgtacgacgt ggcgttgctc
     2161 gatctcgacg gtgtcgtcta catcggacca gatccgatcc cgggtgcggc tgatgcgctt
     2221 gccaaagctc gcgcggctgg tatgcggctg gcgttcgtca caaacaacgc gtcccgcgat
     2281 ccggccgctg tcgccgcgca tctcgttgag ctcggggtgc cggcaacggc cgacgacatc
     2341 gtcacgtcgt cccaggcagc ggcggcggtg atcgtcgaac gcctcggtgt cggcgccacc
     2401 gtgttggtga ccggcagcgc tgccctgcgc gcagcggtcg agc
//
LOCUS       DASWVF010000842         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_24353, whole genome shotgun sequence.
ACCESSION   DASWVF010000842 DASWVF010000000
VERSION     DASWVF010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24353"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGD55_14920"
     CDS             <1..666
                     /locus_tag="VGD55_14920"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0871689.1"
                     /translation="HHLRSRLTGSGVEQLVYTPQYFAISQSAGSDPAIYSKVTAEWQV
                     LVEVIDELLVAVKDLISRWVHDGLYVVLDTNKLVQTIESFEEIDWRANLNVSIGLADV
                     IHLVVPLVVIDELDRLKRTPKRSEARRALKAIEARVSAPGQRVGLQRRDPYLPVTFEV
                     LAEPLGHERLSNNDSEIVARATWLRDLGLNVAVATWDTGTRFRASGEGLRVVQLPDEL
                     ELP"
     gene            complement(686..1075)
                     /locus_tag="VGD55_14925"
     CDS             complement(686..1075)
                     /locus_tag="VGD55_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871690.1"
                     /translation="MFAVDQATGATRPSSGAFKPDADGVSAYSEMALGAEGLSPSALF
                     LRSTGALARLPVGSIRRIAGLDAVGDPWPRDVPDHPTNAAHALITGMAGISKGARLDL
                     QKAIAEMPGLSIVRPLGPASPTAPPAS"
     gene            complement(1174..1677)
                     /locus_tag="VGD55_14930"
     CDS             complement(1174..1677)
                     /locus_tag="VGD55_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871691.1"
                     /translation="MGTLPDRDERRVTVKLLDRVSRTAVHAAWLSHATDTLNRLLALE
                     QAWDGQRARPVTPAAVTGMIAILAVVMDDDLASPQIFPLPDGGLQAEWHAGGHDVEIE
                     IEGDGAVYVIATTPDRHQLAHEPVSVLRINTSTPAVDPSSEPTVTALKKALHQLTQAI
                     VDAQFAR"
     gene            complement(1773..>2215)
                     /locus_tag="VGD55_14935"
     CDS             complement(1773..>2215)
                     /locus_tag="VGD55_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871692.1"
                     /translation="ARFDQHFTQFRAFAAEISAGPVEAQQAEVTYINDLGLGGTMPAL
                     DEALTAWTKVDLHHLGEPEEVRFAEVFTVPSMSPLARLYISLDPHKRADGTETTLLTL
                     TVRGHTAGPSPGDVLSFLDTARGHIVTSFSEITSETMQLRWGRK"
BASE COUNT          374 a          693 c          730 g          418 t
ORIGIN      
        1 catcatctgc gtagccgcct cactgggtcg ggggttgagc agcttgttta cactccgcag
       61 tacttcgcga tcagtcaatc cgcgggctcg gacccggcca tctactcaaa ggtgacggcc
      121 gagtggcagg tcctagtcga ggtcatcgac gagcttttgg tcgcggtcaa ggacctgatc
      181 tctcgatggg ttcacgacgg actgtacgtc gtgctcgaca cgaacaaact ggtccagaca
      241 atcgaatcat tcgaggagat tgactggcgg gcgaacctga acgtaagcat cggtctcgcc
      301 gacgtcattc atctagtcgt gccgttggtc gtgatcgacg aacttgatcg cctcaagcgc
      361 acgccgaagc gcagtgaggc tcgacgcgca ttgaaggcga tcgaggcgcg agtgagtgcg
      421 cctgggcagc gggtcggtct ccagcgccgc gatccgtacc tgcccgtcac gttcgaggtg
      481 cttgctgaac cgcttggcca cgagcggctt tcgaacaacg acagtgagat cgtggcgcga
      541 gccacctggc tccgagacct cggcctgaat gtagccgtgg cgacgtggga caccgggacg
      601 cggttccggg ccagcgggga aggtttgagg gtagttcagc tgccggacga gttggagtta
      661 ccctgaccga cgcgcgcggc ctgggtcacg aagctggcgg cgctgtcgga ctggcgggtc
      721 cgagcggtcg cacgatggac agccccggca tctccgcgat ggccttctgc aggtctagtc
      781 gggcgccctt ggaaatgccg gccatacctg tgataagcgc atgagctgcg ttcgttggat
      841 ggtcggggac gtcccgcggc cacggatctc caaccgcgtc gaggccggcg atccgacgta
      901 tcgaaccgac agggagccgt gccagcgcac cggtggaccg caggaacagc gcgctgggag
      961 ataacccctc ggcgccgagc gccatctccg aataggcgga cactccatcc gcgtcaggct
     1021 tgaacgcgcc ggaggatggt cgtgtcgcgc cggttgcctg atcgacagcg aacatgttgg
     1081 gatcgtccgg aatacgtcgg agcagcgagt cccgtgcgct cagcaattgc cccgcttcca
     1141 accgcccgtc ggcatctccg gggccgtcat ggctcaccgc gcgaactgcg cgtcgacgat
     1201 cgcctgggtc agctggtgca gcgctttctt cagcgccgtc accgttggtt cactgcttgg
     1261 gtcgacggct ggcgtactcg tgttgatcct gagcaccgaa actggctcat gcgcgagttg
     1321 gtggcgatcc ggtgtggtcg cgatgacgta gaccgcgcca tcgccctcga tctcgatctc
     1381 gacgtcgtgt ccgcccgcat gccattcggc ttgcaggccg ccgtcgggta gggggaagat
     1441 ctgcggcgac gcgagatcgt cgtccatgac gacggcgagg atcgcgatca tccccgtcac
     1501 agcggccggc gtgaccggcc gtgctcgctg gccatcccat gcctgctcca acgcaagcaa
     1561 gcggttcagg gtgtccgtcg cgtgactcaa ccaggcggcg tggacggccg tgcgcgacac
     1621 cctgtcgagg agcttcaccg tgactctgcg ctcgtcacga tcgggcaagg tacccaccgc
     1681 gaatcccgtc gcggtgtcat cgggcgccga gggacgtggc gttcttggga acgtagtcgc
     1741 agaaggcgcg atcctccgct cgtccagcgc tgtcatttcc tgccccatct cagttgcatc
     1801 gtctccgaag tgatctcact gaaactcgtc acgatgtgcc cccgtgcggt gtctaggaat
     1861 gacagaacgt caccgggcga cggtcctgcg gtatggcctc gaaccgtcaa cgtcaacagg
     1921 gtcgtctcgg tgccatccgc gcgtttgtga gggtccaagc tgatgtagag cctcgcgagt
     1981 gggctcatgc ttgggaccgt gaacacctcg gcgaatcgca cctcttcggg ctcgccgagg
     2041 tggtgcagat ccaccttcgt ccaggcggtc aacgcctcat cgagcgcggg catggtgcca
     2101 ccaagaccca ggtcgttgat gtaggtgact tcggcctgtt gtgcttccac agggcccgcg
     2161 cttatctcgg cggcgaatgc acggaactgc gtgaagtgct gatcgaaccg ggccc
//
LOCUS       DASWVF010000843         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_24370, whole genome shotgun sequence.
ACCESSION   DASWVF010000843 DASWVF010000000
VERSION     DASWVF010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24370"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1674
                     /locus_tag="VGD55_14940"
     CDS             <1..>1674
                     /locus_tag="VGD55_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871693.1"
                     /translation="WGNAGEIGFSYNVTTHILDQMTLNVGYTGSLQTNKYPDNNGEIH
                     DYTLTIGDIYRRDGAPFEINWNDGTDRNTKITNIFNAIMATYSGPAGVPFSTATCSKD
                     SDCAAPGVKANCQCLHDTTTTSSCVTGKTGQCGMINCATDGNCLVNPNNGGGTYFGIR
                     PMVVYIVGSAGVPQPALSTPTLIYNFLTKFEPYSNLPQTITLDQNGPTANGTPIGSTD
                     GGVCIQHIGQTFGDLITHCVAVTGKTDVDTVNTNKIEHGLSHDQEHWTANVLGINQNF
                     TSASVAANPNIVVLDTDVPQPTDTTQDYEFDLRARGALSNDGALQGSGLMFIEWARLM
                     LEDIAVQQAKLYGTGPLTPRKLGDPECVGFTGGAPNYVATNTAIWNGSTFTGTKALCS
                     GVEGLVIPSGGAGDFSADPGLATADPNANLNPTANFDQVGFYASVLHPGDIWGAVCVD
                     ITGYTDCAPYGTSIWSDVLQQAIRVQARGDVLALPTEMRDRRYYFRLFGVAYVRYLKA
                     YGNYNKKYPCAAGAAPATCPVNQFPTGTAGGGLGPSDVVATQVDQEALF"
BASE COUNT          321 a          617 c          490 g          246 t
ORIGIN      
        1 ctggggtaac gcgggcgaga ttgggttcag ctacaacgtc acgacccaca tcctcgacca
       61 gatgaccctg aacgtcgggt acaccgggtc gctccagacc aacaagtacc ccgacaacaa
      121 tggggaaatc cacgactaca cgctgacgat cggcgacatc tatcgtcgtg acggcgcgcc
      181 gttcgagatc aactggaacg acggcaccga tcgcaacacg aagatcacga acatcttcaa
      241 cgcgatcatg gcgacctact cgggcccggc cggcgtgccc ttcagcaccg cgacgtgctc
      301 gaaggactcc gactgcgcgg cgccgggcgt gaaggcgaac tgccagtgct tgcacgacac
      361 gaccacgacc tcgtcgtgcg tcacgggcaa gactggccag tgcggcatga tcaactgcgc
      421 gacggacggc aactgcctcg tcaacccgaa caacggcggc ggcacgtact tcggcatccg
      481 gccgatggtc gtctacatcg tcggctcggc cggcgttccc cagccggcgc tcagcacgcc
      541 gacgctcatc tacaacttcc tcaccaagtt cgagccgtac tcgaaccttc cgcagacgat
      601 cacgctcgac cagaacggtc cgacggcgaa cggcaccccg atcggctcga ccgacggcgg
      661 cgtctgcatc cagcacatcg gccagacgtt cggcgacctc atcacccact gcgtcgcggt
      721 gaccggcaag accgacgttg acacggtcaa cacgaacaag atcgagcacg gcctcagcca
      781 cgaccaggag cactggacgg cgaacgttct cggcatcaac cagaacttca cgtcggcgtc
      841 cgtcgcggcg aacccgaaca tcgtcgttct cgacaccgac gtcccccagc cgaccgacac
      901 cacccaggac tacgagttcg acctccgcgc ccgcggcgcg ctgtcgaacg acggcgccct
      961 ccagggctcc ggcctcatgt tcatcgagtg ggcgcgcctc atgctcgagg acatcgcggt
     1021 ccagcaggcg aagctctacg gaaccggccc gctgacgccg cgcaagctcg gcgaccccga
     1081 gtgcgtcggc ttcaccgggg gcgccccgaa ctacgtcgcg acgaacacgg cgatctggaa
     1141 cggctcgacg ttcaccggca ccaaggcgct atgctccggt gtcgaaggcc tcgtcatccc
     1201 gagcggcggc gcgggagact tctccgcgga cccgggcctc gcgactgctg acccgaacgc
     1261 caacctcaac cccacggcga acttcgacca ggtcgggttc tacgcgagcg tccttcaccc
     1321 cggtgacatc tggggtgcgg tttgcgtcga tatcaccggc tacaccgact gcgctccgta
     1381 cgggacgtcg atctggagcg acgtgctcca gcaggccatc cgcgttcaag ctcgcggcga
     1441 cgtgctcgcg cttccgaccg agatgcgcga ccgccgctac tacttccgcc tcttcggcgt
     1501 cgcgtacgtc cgctacctca aggcgtacgg caactacaac aagaagtacc cgtgcgccgc
     1561 gggcgcagcg ccggcgacct gcccggtcaa ccagttcccg acgggcaccg ctggcggtgg
     1621 cctcggtccc tccgacgtcg tcgcgaccca ggtcgaccag gaagccctct tctt
//
LOCUS       DASWVF010000844         7870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_380752, whole genome shotgun sequence.
ACCESSION   DASWVF010000844 DASWVF010000000
VERSION     DASWVF010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380752"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..900
                     /locus_tag="VGD55_14945"
     CDS             184..900
                     /locus_tag="VGD55_14945"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY0871694.1"
                     /translation="MFGILWQTSTLMFASVLLTRFKGGLGGFPSAGVLLIIGMRLVAH
                     GLYVLCFYNLAWLPLLVDEGRMDGYFLRPLPVFMQLLLSQFSVNAIGDLVVGLSTLGV
                     ALALVHVHWTLLIALYVVVAVIGGALVEAAVQLMLSCLLLRSPASRVLGTWVDELMST
                     FGNYPLSILPRTVQGLFTFALPLAFVAFFPVEVILGIGAKHGGMSVLAHWSPAVGFVL
                     FLVARRVWSYSLRHYHSAGG"
     gene            complement(918..1958)
                     /locus_tag="VGD55_14950"
     CDS             complement(918..1958)
                     /locus_tag="VGD55_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0871695.1"
                     /translation="MPTAPTLEDVAKAAGVHKATASRALNPATDSRVSEQTVRRVRAV
                     AQRLGYLPNTLAQGLRTARTSTIGVVLPDLTNPLFPPIVRGIEDVLAARGYVALLANT
                     DNDDQREITHIDALRARRVDGFIVATARRDHPLLEAMALEGVAIVLVNRTTDRHDISA
                     VLGNEAAGIAAAVDHLVELGHRSITHLAGPQQLSTGTARADAFRAAMRAHGLRIRRAD
                     IVQCDGYTVRAGFAAAHRLVATNRALTAIVAANDMIAIGALQALHADGLRCPDDVSVV
                     GFNDMPFVDQLSPPLTTVRLPQYEVGVQAAQLLLERLANPATSAKSVVLPVALVARGS
                     TGPYRARAVAAS"
     gene            complement(2099..2434)
                     /locus_tag="VGD55_14955"
     CDS             complement(2099..2434)
                     /locus_tag="VGD55_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871696.1"
                     /translation="MPIVMGAPVGLATGADADDVAGADALVDAATGALAEAAAVEDPP
                     PDEDVAAGVDFFDELQPATTSAVTAKAATDALESGRDRRDACNRLCETIESLHERWVS
                     VRARFLTWP"
     gene            2445..3539
                     /locus_tag="VGD55_14960"
     CDS             2445..3539
                     /locus_tag="VGD55_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0871697.1"
                     /translation="MTGDFSDPGTAAQKGYQTWASVVNAKGGINGRPVQLKIVDDTSS
                     PDQVTTNYQNLISKDKVNFVFGPFSSLLTVPAGQVANRYGYAFIEPAGGGPAVFAEHL
                     PNLFFVQPAPVVSEGKAFASYVLSLPAAQRPKTAAYPELDDPFSGPISETVRAEFEAA
                     GIKTVYKQVYPPELTDLTSIVQKMKTAKPDVVVAGTQSDDAFAMVKAMVQLKFAPNLL
                     FFANGANDPLNFPDKVGAANVNGIFSASDWFPDATTFRSAEFVQAYLAKFGGTAQQID
                     PTSAEAFSAGVLLEEAIAKTGGTDNKAIIAALHQGSWPTPEGNLSWDADGAPQGSDTL
                     VQWQGGKLLAVWPQAAQKVPPMLVPLPWAS"
     gene            3549..4427
                     /locus_tag="VGD55_14965"
     CDS             3549..4427
                     /locus_tag="VGD55_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0871698.1"
                     /translation="MRNFVQAVIIGVLTGGVYALMASGQTLIFGIMKVINLAQGALVI
                     VGAFLSYSLFIHFGIDPFLSILITTPLLFGIGVLIQLAFLRPLRNDDAAEMSLLVTFA
                     VALGIEGVLTLTYKTNYRSIQTSYANKSFDIAGYEISAVRLYAFVLSLVALVVLYLVL
                     ERTRLGRAIRATVQNPTSALLLGVDSKKVAAIGFGLGAATAAAAGAVFGVVYAFNPNS
                     HYDLISRLLSIVILGGLGSIGGAVIGALIMGVASSLVADFGSPVWSDFTFFGVLIVVL
                     LLRPQGLFGTTLRGSS"
     gene            4424..5455
                     /locus_tag="VGD55_14970"
     CDS             4424..5455
                     /locus_tag="VGD55_14970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0871699.1"
                     /translation="MTKFGVARGAVFVALLVSFPFVVHAHWILNIAVFTLMFAGLASA
                     WNLVGGYAGYPSLGHAAFFGAGAYVEAIWFHHHSIGSGYLPFIALPVVGLIVMVASIP
                     IAWVAMRTRADVFAIVTITVLFMAQTLAFNLRSITGGSQGIGIHPPPFALATFERPFY
                     FAFASILIVAAGITFLTSRSKVGLSLLAVRSDEDKARGIGVHVTLAKLTGFSVSVWLT
                     AMIGAVWAYYVSFVYPQFAVDPLITIAMVLMTFLGGRATLWGPILGAFILAPAQQYLA
                     YTYGASRLYLLAYAAVFLLIMLFLPRGIIPSIADRRFARSRKTRLDVASTGTSGSPGP
                     SQLEQPVAS"
     gene            5452..6183
                     /locus_tag="VGD55_14975"
     CDS             5452..6183
                     /locus_tag="VGD55_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871700.1"
                     /translation="MTALLDVRHLTKRFGGITAVDDCAFEVADGTITALIGPNGSGKT
                     TVFNLITGYVRSDAGDIAFAGRAYTRPEPGALCRRGLVRTFQQARVFPVLSVVENLVV
                     AAVHPWWALATARASKVDRARADELMTDFGLSHVADLPASQLSYGQRKLLEFAAVLMS
                     RPQLVLLDEPTAGVNPTMVATMERHVRERNAEGITFLVVEHDMNVVMRLCDPVIVLDH
                     GAPIAQGAPATVQADPAVLDAYLGQ"
     gene            6192..6974
                     /locus_tag="VGD55_14980"
     CDS             6192..6974
                     /locus_tag="VGD55_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871701.1"
                     /translation="MSDPTTSLPLLVLDGVVAGYGAGDILKGVSLEVREGEVVCLIGP
                     NGAGKSTVLKTISGLLRPTKGSVRFRGEVITKMSIRERLARSIVHVPQDRSLFPAMSV
                     WENVIMGAYSLRDEAEARRRADRAAAAFPIVAKRRHEAAGSLSGGEQKQVEMARSLML
                     DPALILLDEPSIGLDPKSRKVVFDSITQLAADGRTVLLVEQNARSGLAVADRGAVLES
                     GLVRLVGTGSALLLDPEVARLYLGGGAVNAGGAASTSEQAAL"
     gene            6992..>7870
                     /locus_tag="VGD55_14985"
     CDS             6992..>7870
                     /locus_tag="VGD55_14985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0871702.1"
                     /translation="MAGTEVADAPRLGWIGAGRMGAAMARRLLANGHQVSVYNRTRAK
                     AEALMPDGAVVVDTIAELATCDVVFTSVSSSADLEQVLLGEGGLLRQDRVPGIVIDTS
                     TVSAGMSAQVREAATARGCAFLAAPVSGNAKVVAAGRLTLVVSGSDDAYRRVAPLLEV
                     LGSGVTYVGDDESARLVKIAHNVFLGVVTQSLAEILVLVEKGGVSRSAFLEFLNASVM
                     GSTFTKYKSPALTHLDFTPTFTTELLRKDFELGLAEARSMGVAMPVAALTHQLVQAAV
                     GAGHRGVDFASLLLEVA"
BASE COUNT         1235 a         2497 c         2610 g         1528 t
ORIGIN      
        1 cgcgtcgcgt gaccgtgcag ggcggttgac gaagtggcgc tagcgacatc ggtagctgag
       61 ccggcatcgg cactgcgctc gcgcgcgcac cgtgcacgcc acaccgcacg catcgttagg
      121 aaagtcagtg cgctcaacct ccgcgcccag ctggaatatc gcggcaactt cattgcgtcg
      181 atcatgtttg gcattttgtg gcaaacgtcg acgctcatgt ttgcgagcgt gctgctgacg
      241 cgattcaaag gagggctcgg agggttcccg agcgcaggtg tgctgctcat catcgggatg
      301 cggctcgtcg cgcacggcct ttacgtgctg tgcttctaca acctcgcgtg gctgccgctc
      361 cttgtcgacg aaggccgcat ggatggctac ttcttgcggc ctcttccggt attcatgcag
      421 ctcttgctca gccagttcag tgtcaatgcc atcggtgatc tcgtcgtcgg gttgtcgacc
      481 ctcggcgtcg ccctcgcgct ggttcatgtg cactggacgt tgctgatcgc gctctacgtc
      541 gtggttgccg ttatcggggg tgcgctcgtc gaggccgcag tgcaactgat gctgtcgtgc
      601 ttactgctgc gctcgccggc atcacgcgtg ctcggcacct gggtcgacga gttgatgtcg
      661 acgttcggta actacccgtt gtcgatcctg ccgcgcacgg tgcaggggct gtttacgttc
      721 gccttgccgc tcgcattcgt ggcgttcttc cccgtcgagg tcattctcgg gatcggtgcg
      781 aagcacggtg ggatgtcggt gctggcgcac tggtcgcccg ctgtcggctt cgtgttgttc
      841 ctggtggcac ggcgggtatg gagctacagc cttcggcact accactctgc cggtggctga
      901 cgcgtttcaa ccacaggtca actcgccgca accgcgcggg cgcgataggg cccggtcgag
      961 cctcgcgcga ccaaagcgac tggcaggacc actgacttgg cgctagttgc cgggttagcc
     1021 agccgctcga ggagaagctg cgcggcttgc accccgacct cgtactgagg gagccggacg
     1081 gtggtcagcg gcggactcaa ctggtcgacg aacggcatgt cgttgaaccc gacgacggag
     1141 acatcatcag ggcagcgcag gccgtcagca tgaagcgcct ggagtgcgcc gatggcgatc
     1201 atgtcgttgg cggccacgat cgcggtcagc gctcggttag tggcgacgag ccggtgcgcc
     1261 gcggcaaaac cggcgcgcac ggtgtaaccg tcgcactgga cgatgtcggc ccgccggatg
     1321 cgcaggccgt gcgctcgcat cgcagcccga aacgcgtcgg ctcgggcggt gccggtcgac
     1381 agctgctgcg ggccggccag gtgggtgatc gagcggtgcc cgagctcgac caggtggtcg
     1441 acggcggctg cgattccggc ggcctcgttg ccgagcacgg cggagatgtc gtggcgatcc
     1501 gtagtgcggt tcaccagcac gatcgccacg ccctcgagtg ccatcgcctc gagaagggga
     1561 tggtcgcggc gagcagtcgc gacgatgaag ccgtcgaccc tacgggcacg cagcgcgtcg
     1621 atatgagtga tctcgcgctg atcgtcgttg tcggtgttgg ccagcaaagc gacgtagcct
     1681 cgggctgcga ggacgtcttc gatccccctc acgatcgggg gaaagagcgg gttggtgagg
     1741 tcgggcaaca cgacgccgat cgtcgacgta cgtgcggttc gaaggccctg cgcgagcgtg
     1801 ttcggcaggt agccgagccg ctgcgcgact gcccgaaccc ggcgtaccgt ttgttcgctc
     1861 acccggctgt cggtcgccgg gttcagggcg cgtgacgcag tggccttgtg gacgccggca
     1921 gccttggcga cgtcctcgag agtcggggcg gtcggcacgt cccgaccgta gcgctcgggt
     1981 ggtatccgca caaccgattg cgcgctggac ccttgacaac gctccccacc gggtcttgaa
     2041 tctcggccaa taggtgcaca accgattgca caaactgtcg actgagccct cctgcaagct
     2101 acggccaagt caagaaccgc gcccggacac tcacccacct ctcgtggagg ctttcgatgg
     2161 tctcgcacaa tcgattgcac gcatcccgcc tatcgcggcc gctttccaag gcgtcggttg
     2221 ctgcttttgc ggtaaccgca ctcgtcgtcg ccggctgtag ctcgtcgaag aagtcgaccc
     2281 cggctgccac gtcttcgtcg gggggtgggt cctccaccgc ggccgcctcg gcgagcgcgc
     2341 cggtggctgc gtcgacgagt gcatccgcgc ccgcgacatc atcggcgtcg gcgcccgtcg
     2401 cgagcccgac cggtgcgccc atcacgatcg gcatctcgct ctcgttgacc ggtgacttct
     2461 ccgatcccgg cacggccgcg cagaagggct accagacgtg ggcgagcgtc gtaaatgcaa
     2521 agggcgggat caacgggcgc ccggtgcagt tgaagatcgt cgacgacacg tcgagccccg
     2581 accaggtgac gacgaactac cagaacctga tcagcaagga caaggtcaac ttcgttttcg
     2641 gcccgttctc gagcctgctc actgtgcctg ccgggcaggt cgccaaccgg tacggatacg
     2701 cgttcatcga gccagccggt ggcggtcccg ccgtcttcgc agagcacctg ccgaacctct
     2761 tcttcgtgca gcccgccccg gtggtgtctg agggcaaagc gttcgcttcc tacgtgttgt
     2821 cgctgccagc tgcgcaacgt ccgaagacag ctgcgtaccc cgagctcgac gacccgttct
     2881 ccgggcccat ctcggagacc gtgcgcgcgg agttcgaggc ggctggcatc aagaccgtgt
     2941 acaagcaggt ctacccgcct gagttgaccg atctcacgtc tatcgtgcag aagatgaaga
     3001 ccgcaaagcc ggacgtcgtg gtcgccggta cccagtctga tgacgcgttc gcgatggtca
     3061 aggcgatggt gcaactgaag ttcgcaccca acctgctgtt cttcgccaac ggcgccaacg
     3121 acccgttgaa cttccctgac aaggtcggtg cggccaacgt caacggcatc ttctcggcgt
     3181 ccgactggtt ccccgatgcg acgaccttca gaagcgcgga gttcgtccag gcatacctcg
     3241 cgaagttcgg tggaacggcg cagcagatcg acccgacatc ggccgaggcg ttctcggcag
     3301 gagtcctgct cgaagaggcg atcgcgaaga ccggcggcac cgacaacaag gccatcatcg
     3361 cggcccttca ccagggcagc tggcccactc ctgagggcaa cctctcgtgg gacgccgacg
     3421 gcgcacctca gggcagcgac acgctggtcc agtggcaggg cggaaagcta ctcgcggtgt
     3481 ggccgcaagc tgcgcagaag gtcccgccga tgttggtgcc cctgccgtgg gcatcgtagg
     3541 cgcggctgat gcggaacttc gtccaagccg tcatcatcgg cgtgctcacc ggcggtgtgt
     3601 atgccctgat ggcgtcgggc cagaccctga tcttcggcat catgaaggtc atcaatctgg
     3661 cccaaggggc gctggtgatc gtcggcgcgt tcctgtccta ctcgctcttc atccatttcg
     3721 ggatcgaccc gttcctctcg atcctcatca cgacgccgct actgttcggc atcggcgtgt
     3781 tgatccagct ggcatttctg cggccactgc gtaacgacga tgcggcagag atgtcgctgc
     3841 tcgtgacgtt cgcggttgcg ttgggcatcg aaggggttct gacactcacc tacaagacca
     3901 actacaggtc gatccaaacc agctatgcga acaaatcgtt cgacatcgct ggctatgaga
     3961 tcagcgcggt gcggctgtac gcgttcgtct tgtcgctggt ggcacttgtg gtgctctacc
     4021 tcgttctcga gcgcacgagg ctggggcgcg cgatccgggc caccgttcaa aacccgacat
     4081 cggcactgct tctaggtgtc gattcgaaga aggtcgcggc gatcggtttc ggcctcgggg
     4141 ccgcaaccgc agccgcggcc ggggcggtgt tcggggtggt gtacgcgttc aatcccaaca
     4201 gccattacga cctgatctcg cgcctgctgt cgatcgtgat tctgggtggc ctcggcagca
     4261 tcgggggtgc cgtcatcggt gcgctcatca tgggagtcgc ctcgagcttg gtcgccgatt
     4321 tcgggtcgcc ggtatggagt gacttcacgt tcttcggcgt tctgatcgtc gttctgctcc
     4381 tcagaccgca gggcctgttc gggaccacgc tgcggggctc atcgtgacca agttcggcgt
     4441 cgcgagaggc gcggtgttcg tggcgctgct cgtgtcgttc ccgttcgtcg tgcacgcgca
     4501 ctggatattg aacatcgccg tgttcacgct gatgttcgct ggccttgcgt cggcgtggaa
     4561 tctcgtcggc ggctacgcgg gctatccgtc gttgggtcat gccgcgtttt tcggcgccgg
     4621 cgcctacgtc gaggccattt ggttccacca ccattcgatc ggctccggct atttgccgtt
     4681 catcgcactg ccggtggtcg gcttgatcgt gatggtggcg agcattccga tcgcgtgggt
     4741 cgcgatgcgc acgcgcgccg acgtgttcgc gatcgtcacg atcacggtgt tgttcatggc
     4801 gcaaacactt gcgttcaact tgcgcagcat tacgggcggc tcgcagggga tcggcataca
     4861 cccgccgcca ttcgcactcg ccaccttcga gcgacctttc tatttcgcgt tcgcatcgat
     4921 tctgatcgtc gccgcaggca tcaccttctt gacgtcgcgc agcaaggtcg gtctttcgct
     4981 gctcgccgtg cggtccgacg aagacaaggc acgcggaatc ggcgtgcatg tcacgctcgc
     5041 gaagctgaca ggtttctccg tgtcggtctg gttgacggcg atgatcggcg cggtctgggc
     5101 ctactacgtc tcgttcgtgt acccacagtt cgcagtcgac ccgcttatca cgatcgcaat
     5161 ggttctcatg acgttccttg gcggtcgagc gacgctgtgg gggccgattc tcggcgcgtt
     5221 catcctcgcc cccgcgcagc agtacctggc gtatacgtac ggcgcgagca ggctttacct
     5281 cctggcgtat gcagcggtgt tcctcctcat catgctgttc ctcccgcgcg gcatcattcc
     5341 gagcattgcg gatcggcgat tcgcgcgcag caggaaaacc cgtctcgatg tcgccagcac
     5401 tggtacctcg ggttcgccgg ggcctagcca actcgaacag ccggtggcgt catgaccgca
     5461 ctgctcgacg tccggcatct gacgaagcga ttcggcggca tcaccgccgt cgacgattgc
     5521 gcgttcgagg tcgccgacgg caccatcacg gcgcttatcg gtcctaacgg atcaggcaag
     5581 acaacggtgt tcaacctgat caccggctac gtgcggtcag atgccggtga cattgcgttc
     5641 gcgggccgtg cgtacacgcg gccggagcca ggagcgctgt gtcggcgagg cctggtgcgc
     5701 acgttccagc aggctcgggt gtttcccgtg ctgagcgttg tcgagaactt ggtggtcgcg
     5761 gcggtgcacc cgtggtgggc gctggcgact gcgcgcgcct ccaaggtcga tcgagcgaga
     5821 gccgacgagc tcatgaccga cttcgggctc tcccacgtcg ccgatctgcc ggcctcgcag
     5881 ctctcctatg gccagcggaa gctgctcgag ttcgctgcgg tcttgatgag tcgcccgcag
     5941 ctggtgttgc tcgacgagcc gactgcagga gtgaacccca cgatggtcgc gacgatggag
     6001 cggcacgtgc gcgaacgcaa cgccgagggc attaccttcc tggtcgtcga gcacgacatg
     6061 aacgtcgtca tgcggttgtg cgatccggtc atcgtgctcg accacggtgc accgatcgca
     6121 cagggcgcgc ctgcgactgt tcaggccgac ccggcagtcc tcgacgcata cctgggccag
     6181 tgatggcggc aatgagcgac ccgacgacct cgttgccgtt gcttgtgctc gacggtgtcg
     6241 tcgccggtta cggtgcgggc gacatcttga agggcgtcag cctcgaggtc cgcgagggcg
     6301 aagtggtctg cctcatcgga cccaacggcg ccggcaaatc gaccgtcctc aagacgatca
     6361 gtggcctctt gcgaccgacc aaagggtcgg tgcgattccg cggcgaagtc atcacgaaga
     6421 tgtcgattcg cgagcggctg gccaggtcga tcgttcacgt gccgcaggac cgcagcctgt
     6481 tccccgcgat gagcgtgtgg gagaacgtga tcatgggtgc gtactcgctc cgcgacgagg
     6541 cagaggcacg gcgtcgggcc gaccgtgcgg cggcggcatt ccccatcgtc gcgaagcggc
     6601 ggcatgaggc agcgggctca ctttcgggag gcgagcagaa gcaggtcgag atggcccgct
     6661 cgctgatgct cgatcccgcg ctcattctgc tcgacgagcc gtcgatcggc ctcgacccta
     6721 agtcacgcaa ggtcgtcttc gactcgatca cgcaactcgc cgccgacggt cgcaccgtgt
     6781 tgctcgtcga gcagaacgcg cggtcaggcc ttgcggtcgc cgaccgtggc gcggtgcttg
     6841 aatccggcct ggtccgcctg gtcggaacgg gcagcgcgtt gttactcgac cccgaggtcg
     6901 cccgcctcta cctgggtggc ggcgccgtga acgcaggcgg agcagcctcg accagcgagc
     6961 aagcggcact atgacgacga gcggagagca catggccggg accgaggtgg ccgatgcccc
     7021 acgactgggc tggataggcg ctggtcggat gggcgcggcg atggcgcgcc gactgctcgc
     7081 caacgggcac caggtgtcgg tctacaaccg aacccgagca aaggcagaag ccctcatgcc
     7141 ggacggcgcg gttgtggttg acaccattgc cgaactggca acgtgcgacg tggtgttcac
     7201 gagcgtctcg tcatcggctg accttgagca ggtgctgctg ggtgagggtg ggttgttacg
     7261 ccaagaccgg gtgccaggaa tcgtgatcga cacgagcacg gtgagcgcgg ggatgtccgc
     7321 gcaggtgcga gaggcggcta ccgcaagagg ttgcgcgttc ctggccgctc cggtgtcggg
     7381 caatgccaag gtcgtagcag ctgggcggtt gacgcttgtc gtctccgggt ctgacgacgc
     7441 ctatcgacgg gtcgcacctt tgctcgaggt actcggcagt ggtgtcacgt acgtcggcga
     7501 cgacgagtcg gcgcggctgg tgaaaatcgc gcacaacgtc ttcctcggtg tggtgacgca
     7561 gtcacttgcc gagatcctgg tgctggtcga gaagggcggc gtcagccgtt ctgcgttcct
     7621 cgaattcctc aacgcctcgg tcatgggctc gacgttcacg aagtacaagt cgcctgcact
     7681 cacccacctc gacttcacgc cgacgttcac gaccgagttg ctgcgcaagg acttcgagct
     7741 cgggctcgcc gaggcacggt cgatgggtgt cgcgatgccg gtcgcggcgc tgacgcacca
     7801 gctcgtgcag gcggcggtag gggccggcca tcgcggtgtc gacttcgctt cgttgctgct
     7861 cgaagtcgcg
//
LOCUS       DASWVF010000845         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_151531, whole genome shotgun sequence.
ACCESSION   DASWVF010000845 DASWVF010000000
VERSION     DASWVF010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151531"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGD55_14990"
     CDS             <1..521
                     /locus_tag="VGD55_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871703.1"
                     /translation="ESQLPDGAPDYVQKMAEPYYATAAAWYETIRIGLTAGELYDVTF
                     SRIGDPFYGVYLNPGHFIHLDEWPSSPVSSGSPVVFTSGNALQLDIIPITGSAYHTAQ
                     IEDGIVLADAELRAEIAAQYPDAWSRIEQRRSMVTKVFGIDLHEEVLPLSNAAAYFPP
                     LWLAPQLAMVRV"
     gene            1070..>2003
                     /locus_tag="VGD55_14995"
     CDS             1070..>2003
                     /locus_tag="VGD55_14995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0871704.1"
                     /translation="MTIRRKLDTYIGIAVLVAALLVALSITNAVQHGLSIALLPVLVL
                     ASVLIIGELRPIRISHGDGSVDEVTISSSFSLALVLTGPLIVALGAQTIATLLDDVRQ
                     RKDLRRIVFNQAQYIITLSASRLAYSAITRRGFFEDARDLRPRDIVGALAAAVVFFVV
                     NHVITTIVIAISLGENPVQRMPMDLRYHLSTSGVLLALAPVLVIAVDFSAFALPLLVL
                     PIAAVHKSAKLAIQREEESLHDSLTGLPNRTLFHRTASTMLARASVTGSSTALMIVDL
                     DHFKEINDTLGHHIGDQVIEAAADRLSVALGANAM"
BASE COUNT          325 a          650 c          604 g          424 t
ORIGIN      
        1 acgagagcca gctgcccgat ggggcgcccg actatgtgca gaagatggcc gagccgtact
       61 acgcgacggc agctgcctgg tacgagacga tccgcatcgg gctgacggcg ggagagctct
      121 acgacgtgac gtttagccgg atcggtgatc cgttctacgg cgtgtacctg aaccctggcc
      181 acttcattca cctcgacgag tggccgtcgt cgccggtcag ctccggatcg cccgtggtgt
      241 tcacctccgg caacgcgctg cagttggaca tcatcccgat caccggcagc gcgtaccaca
      301 cagcgcagat cgaggacggc atcgttttgg cggacgccga gttgcgggcg gagatcgcgg
      361 cgcaatatcc cgatgcgtgg agccgcatcg agcaacggcg gtcgatggtc acgaaggtgt
      421 tcgggatcga cctgcacgaa gaggtcctgc ccctctcaaa tgccgccgcc tatttcccgc
      481 cgctgtggct agctccgcag ctcgccatgg tccgcgtctg acgtttgcca cacgcacccg
      541 acaattcacc ctaaattcgc cgtaccatct gcgtcgtttc ggcctgttcg gcaccctgag
      601 tcggcatctg cgctccggca ggcagcagtc ggctatccgc ggctagcaaa cgggtgcaga
      661 gagttccctc aggtgggcga tgggttccgc tgcaggcgct gccgacaact cttacatcag
      721 ggtggctgcc gcaaattcac gcggcgcctg gtttgccgtg gggaggttga tatgtcgcgt
      781 tcacgtttgt tcgttcgtgt cgtggggtcc gctacgtctt tggtagccct cgttgccgtc
      841 gtcggtgctg gctggaagtg gtagttcgct gctgctgcag ctgaccccca ctctcagtcg
      901 cttacggaga ggagcctaag tcgtgaaggc acaactcatg acaagcgttg tcgacgccga
      961 tggagccttg ggggctgtcc tcgtcgccgg ccacaaatgg cgctaggtcg gccacgccga
     1021 ggttggcgct agatcactag atcttgcagg gaggagggca gcaccgccga tgaccattcg
     1081 ccgcaaactc gatacctaca ttggcatcgc ggtcctggtt gctgccctcc tcgtcgcact
     1141 ttcgatcacc aacgcggtcc agcacggcct gtccatcgcg ttgcttccgg ttctggtgct
     1201 cgcgtcggtg ctgatcatcg gggagcttcg cccgatccgg atctctcacg gtgacggcag
     1261 tgtcgacgag gtcaccatct ccagctcgtt cagcctggcc ctcgtattga ccggcccact
     1321 gatcgtggcg cttggtgcgc agacgatcgc gaccctgctc gatgacgtgc gccagcgcaa
     1381 ggacctgcgc cggatcgtct tcaaccaggc gcagtacatc atcaccttga gcgcctcgcg
     1441 tctcgcgtat tccgccatca cacgtcgcgg tttcttcgag gatgctcgtg atctgcgacc
     1501 acgcgacatt gtcggcgcgc tcgctgctgc ggtcgtcttc tttgttgtca accacgtgat
     1561 taccaccatc gtcatcgcca tctcgttggg tgagaacccg gtccagcgga tgccgatgga
     1621 cttgcgctat cacctgtcga cgtccggtgt cttgctcgcc ttggcgccag ttctagtgat
     1681 cgctgtcgac ttctccgcgt tcgcgctgcc gctactggtg ttgccgatcg ccgcagtcca
     1741 caagagcgcg aagctcgcca ttcagcgtga agaagagtcg ctgcacgatt ccctcaccgg
     1801 gctgcccaac cggacgttgt ttcaccgaac cgcgagcacc atgttggctc gcgcttcggt
     1861 cactggctct tcgacggcct tgatgatcgt cgacctcgac cacttcaaag aaatcaacga
     1921 caccctcggc caccacatcg gcgaccaggt catcgaggct gctgccgacc ggctgtcggt
     1981 ggcgctcggt gccaatgcga tgg
//
LOCUS       DASWVF010000846         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_329504, whole genome shotgun sequence.
ACCESSION   DASWVF010000846 DASWVF010000000
VERSION     DASWVF010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329504"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGD55_15000"
     CDS             <1..588
                     /locus_tag="VGD55_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY0871705.1"
                     /translation="DRLTEATDDFVYDAVVMDAPPTGRIARFLNVNSEVASLARVGPV
                     NRQAESIMRLIRSPQTVVHLVTMLEEMPVQETLDGINELHEFDLPVGGIFVNRVHEPL
                     LPKAELKAASTGAIDLTALARGLKSAGLPAGDAITAGLSVEAEDHALQLAIERKLRLR
                     LRAAARPTYQLHDLDTTDIGRLYALAAELVEQGAA"
     gene            585..1748
                     /locus_tag="VGD55_15005"
     CDS             585..1748
                     /locus_tag="VGD55_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEY0871706.1"
                     /translation="MTHLDIEALISDPGTKIVVCCGSGGVGKTTLAAALGVRAAEQGR
                     RVVTLTIDPARRLAQSLGLQELDNVPRRVAGMKLKKRVDGAKNEGELHAMMLDMKRTF
                     DEIVEAHASPAKAAQILANPFYQSLSSSFAGTQEYMAMEKLGQLRASGQWDLIVVDTP
                     PARSALDFLDAPARLARFLDGRFIRMLLVPAKVGGRGYTKVMQAGLGLVTGTMMKLLG
                     TQLLTDVQTFVAAMDTMFGGLRQRADATAKLLRAPGTVFVVVASPEPDALREASYFVD
                     RLVEEDMPLAGVVVNRVHRSTVPQITAVNALAAAEALEDGELAGDADSALTAGVLRVH
                     AQRLTMQATEQRRVDGFALAHPNVLLTSARALSQDVHDLNGLRTLGDALVEPG"
     gene            complement(1756..2064)
                     /locus_tag="VGD55_15010"
     CDS             complement(1756..2064)
                     /locus_tag="VGD55_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY0871707.1"
                     /translation="MAWTTDWTTRAGCKNADPDALFVQGAAQNRAKAVCFGCPVRAEC
                     LADALDNRVEFGVWGGLTERERRALLRRRPNVTSWTNLLSTAMSDFERESAETYSATG
                     "
BASE COUNT          391 a          680 c          707 g          362 t
ORIGIN      
        1 gaccgattga cggaagcaac agatgacttc gtctacgacg ccgtcgtgat ggacgcaccg
       61 ccgaccggac gcatcgcacg gttcctcaat gtcaacagcg aagtcgccag cctggctcgc
      121 gtcggcccgg tgaaccggca agccgaatcc atcatgcggc tgatcagatc gccgcagacc
      181 gtcgtccatc tcgtcacgat gcttgaagag atgcccgtac aggaaaccct cgacggcatc
      241 aacgaactgc acgagttcga cctgccggtc ggcggcatct tcgtcaaccg cgtccacgag
      301 ccgttgttgc caaaggccga gttgaaggcg gcgagtacgg gcgccatcga tctcacggcg
      361 cttgcacgcg ggctcaagtc agcgggccta cctgcaggag atgcgatcac tgcgggcctg
      421 tcggtagaag cggaagatca cgcgcttcag cttgcgatcg aacgcaagct gcggctgcgc
      481 ttgcgcgcgg ccgcacggcc gacctaccaa ctgcacgacc tcgacaccac agacatcggt
      541 cgcctctacg ccctggccgc cgaactcgtc gagcagggtg ccgcatgacg catctcgata
      601 tcgaagcgct gatctccgac cctggcacga aaatcgttgt ctgttgcggc tccggtgggg
      661 tgggaaagac aacgctggcc gcagcgctgg gcgtccgcgc cgcggaacag ggcaggcgcg
      721 tggtgaccct caccattgat ccggctcggc ggcttgcaca gtcgctcggc ctgcaagagc
      781 tcgacaacgt gccgcgcaga gtcgctggaa tgaagctcaa gaagcgggtg gacggcgcaa
      841 aaaacgaggg cgagctacac gcgatgatgc tcgacatgaa gcgcacgttc gacgagatcg
      901 tcgaggcgca cgcgtcaccg gcgaaggcgg cccagattct ggccaacccg ttttatcaat
      961 cactgtcatc gagtttcgcc ggtacacagg agtacatggc gatggagaaa ctcggccagc
     1021 ttcgtgcctc gggccagtgg gatctgatcg tggtcgacac tccgccggca cggtcggcac
     1081 tcgacttcct tgatgcgcct gcacgattgg cgcgctttct cgacgggcga ttcatccgga
     1141 tgctgctggt gccggcgaag gtcggtggcc gcggctacac caaggtgatg caggccgggc
     1201 tcggtctcgt caccggaacg atgatgaagc tgctcggcac acagctgctg accgacgtcc
     1261 agacttttgt cgctgccatg gacaccatgt tcgggggctt aaggcaacgt gccgacgcca
     1321 ccgcgaagct gttacgcgcg cccggaaccg tgttcgtcgt ggtggcgtcg ccggagcccg
     1381 atgcgctgcg cgaggcgtcg tatttcgtcg accggctggt cgaagaagac atgccgcttg
     1441 cgggtgtggt ggtgaaccgg gtacatcgca gcacagtgcc gcagatcacc gcggtgaatg
     1501 cgctggcggc agcggaagcc ctggaggacg gcgagctggc cggcgatgcc gacagcgcgc
     1561 ttacggctgg agtgcttcgg gtgcatgcgc agcggttgac gatgcaagcg acggagcagc
     1621 gcagagtcga tggtttcgcc ctcgcgcacc cgaacgtgct gctgacgtca gcaagagcgc
     1681 tgtctcagga tgtgcacgat ctgaacggcc ttcgcacgct cggcgacgcg ctcgtcgaac
     1741 ctggctaaga cggactcagc cggtggcgga gtaggtctcg gcggattcgc gctcgaagtc
     1801 cgacatcgcg gtgctgagca ggttcgtcca cgaggtgacg ttcgggcggc gacgcaagag
     1861 cgcgcgccgc tcccgctcgg tcaggccgcc ccacacaccg aactcgacgc ggttgtcgag
     1921 agcgtctgcc aggcactcag cccgaaccgg gcaaccgaag cagacggcct tcgctcggtt
     1981 ctgcgcggcg ccctggacga acaaggcgtc cggatcggcg ttcttacacc ctgcgcgggt
     2041 ggtccagtct gtggtccaag ccatcgggag ccccgtccgt tgctgtggat ttctcgagct
     2101 accgtcccca ggtgggctcg atcccccgac gttacggact
//
LOCUS       DASWVF010000847         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_176725, whole genome shotgun sequence.
ACCESSION   DASWVF010000847 DASWVF010000000
VERSION     DASWVF010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176725"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGD55_15015"
     CDS             complement(<1..188)
                     /locus_tag="VGD55_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0871708.1"
                     /translation="MAAIHTRMDPDERRDQVLAAALVEFAEGGLAGTSTEAIAVRAGI
                     SQPYLFKMFGTKRDLFIAA"
     gene            256..3525
                     /locus_tag="VGD55_15020"
     CDS             256..3525
                     /locus_tag="VGD55_15020"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0871709.1"
                     /translation="MRIELLGPLDVRVGESGSEHAVELAGARLRGLLIRLAVDAPRPT
                     SAATLVDALWGDEPPLDPANALQSLVSRLRRGLGDSDLVQQVPGGYRLSAERDDVDLH
                     RFTDLARDGREALRRGQLEAAAGLLRDALAVWHGPTPESWGDTTDSVAHQTMLCEQRL
                     DVTADLIEAEIALGHHGDVVAELELLAAQFPLRERVIGLLMRALAGAGRQAEALARYE
                     KVRRDLSDELGVDPSAELQATHLALLRGELDTVAANTRDAATDAARSPARRTNVKTAL
                     TSFVGRDDEIARIGKLLSEVRLITLVGPGGAGKTRLASVAVTAVLDRVPDGVWMVELA
                     PVTDPADVPSAVLDALDVREAALVDLARPTVLTSMRDVTTRLVDALADRECVLLLDNC
                     EHVIDAAARLADDLLAQCASLRIVTTSREPLGIVGETLVTVPPLPQPAVDAYPEDALA
                     HPAVLLFADRAVAVQPDFVVDARTVAPVIEIVRRLDGLPLAIELAAARLRTLPVDEIA
                     ARLTDRFRLLTGGSRTAVPRHRTLRAVVEWSWDLLSVPERLLAERLAVFAGTIATADA
                     IAVCADDQLGSDDIDDLLDSLVDKSLLQASALAGTPRYRMLETLREFGLERLTAHGEV
                     GDMRRRHAARFSELVREAGPHTRTAGQLPWMARLEDERDNILAALRFLCEDDQAQAAL
                     EVAVEMATYWMFSGRHADASSWLEFALQAKGDAAVITRLGAEALQRINSVASSFTRPP
                     DEVETSLASLRDLGERIDAVEIPPDSFLFMLRPVIAMFADDRDAVDRLVNEGLASTDA
                     WVAASLRMFRAAMAENNGDVATMRSDVQIALAQFRALGERWGQASCLGILGQLKTMDG
                     DLDGAIAAIEEAISLSEELGGHDDAIMMNIRLADLRMRTGDIDGARASVAKMQELTDL
                     SALSHQAIVISLVRAEIALFDGDLDQARRLRDECLRRISQVSEVHPAQGHIAAIVLAF
                     AARLSLEAGDLDTARDNVARAYRYARGTKDMPIVAMIGVTAAAAARAEGRLTDAAELL
                     GASASLRGAADATQPEIAKLTARLTKELGATVFIAAYGSARDLDLEAACARLDAIVAP
                     L"
     gene            complement(3529..>4319)
                     /locus_tag="VGD55_15025"
     CDS             complement(3529..>4319)
                     /locus_tag="VGD55_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871710.1"
                     /translation="NESEQPLSRRWRTVRHALALAKRSLIKTARTPEALIDVTLQPVI
                     FLLLFTYVFGGALGGGSQHAYLEYLLPGLLGQTIALAGVALGQNLNADIEKGVFDRFR
                     SLPIARSAPLVGAVMADFMRYLIVCVMTLGFGYIIGFRIQTNVLAMLAAIGISIAFAL
                     CFCWISVFVGMIARTSGAVQGIMFLLVLPLSFGSSVFVKVSTMPGWLQGFVKVNPLTH
                     LVGTVRGLVLGGPVTYHLLWTLLWMAILLVTFVPLALRAYKRRA"
BASE COUNT          760 a         1453 c         1412 g          694 t
ORIGIN      
        1 gccgcgatga acaggtcgcg cttggttccg aacatcttga acaggtaggg ctgggagatg
       61 ccagcacgca ccgcgatggc ctcggtcgac gtcccggcga gaccgccttc ggcgaactcg
      121 acgagtgcgg ccgcgagcac ctgatcacgt cgctcgtcag ggtccatccg ggtgtggatt
      181 gcggccatgc gactaggtta gtggtcactc actaacctag tcaagttgat acgccgtagc
      241 tgtcatagtt gtcgagtgcg gatcgaactt ctgggccccc tcgacgtccg tgtgggcgaa
      301 tcgggctccg agcacgccgt cgaactggcc ggtgcgcggc ttcgcggcct cctgatcagg
      361 ctcgctgtcg acgccccgcg acccacctca gcggcgacgc tggtcgatgc gctgtgggga
      421 gacgagccgc cgctcgaccc ggccaatgcg ctccaatcgc tggtgtcgcg gctgcgtcgg
      481 ggtctcggcg actccgatct ggtgcagcag gttcccggcg ggtaccggtt gagcgccgag
      541 cgcgacgacg tcgacctgca ccgtttcacc gaccttgcgc gagacggccg ggaggcgttg
      601 cgacgcggac agctcgaagc agcggccggc ctgcttcgcg acgcgctcgc cgtctggcat
      661 ggcccgaccc cggaaagctg gggtgacacc actgattcgg tggcccacca aacgatgctg
      721 tgcgaacaac gcctcgacgt gacggccgac ttgatcgagg ccgagatcgc gttaggtcac
      781 cacggcgacg tggtcgccga actcgaactc ctcgcggcgc aattcccgct gcgcgagcgg
      841 gttatcgggc tgctcatgcg ggcgctcgcc ggcgccgggc ggcaggccga ggcactcgcg
      901 cgctacgaga aggtccgccg cgacctcagc gacgagctcg gcgtcgaccc ttccgccgag
      961 ctgcaagcca cccacctggc actgctacgc ggcgaactcg acaccgtggc agcgaacacg
     1021 cgcgacgccg cgaccgacgc ggcccgctcc cctgctcgcc gaacgaacgt gaaaaccgcc
     1081 ctcacgagct ttgtcggccg agacgacgag atcgcccgca tcggcaagct gctgagtgag
     1141 gttcgcctca tcaccttggt cggacctggt ggcgccggca agacgcggct ggcctcggtt
     1201 gctgtcaccg cggttctgga tcgagtaccg gacggcgtct ggatggtcga gcttgccccg
     1261 gtcaccgatc ctgctgacgt tccctccgcc gtccttgacg cactcgacgt ccgtgaggcg
     1321 gcgctcgtcg acctggcaag gccgacggtg ctcacgtcga tgcgcgatgt gacgacccga
     1381 ctcgtcgacg ccctggctga tcgcgagtgc gtcctgcttc tcgacaactg cgagcacgtc
     1441 atcgacgcag cggcccggct agccgacgac ctcctcgcgc agtgcgcgag cctacgcatc
     1501 gtgacgacca gccgcgaacc gctcgggatc gtcggcgaaa cgctcgtcac cgttcccccg
     1561 ttgccgcaac cagctgtcga tgcgtatcca gaagacgcgc tggcccaccc agcggtcttg
     1621 ctgttcgccg acagggcagt agccgtgcaa cccgacttcg tggtcgacgc acgcacggtc
     1681 gctccggtga tcgagatcgt tcgccgactc gacggtcttc cgctggcaat cgaactcgct
     1741 gcagcgcgcc ttcgaacgct gccggtcgat gagatcgcgg ctcggctgac cgatcgattc
     1801 cgccttctca ccggcggtag ccggacggcg gtaccgcgcc atcgcacgct gcgtgcggtc
     1861 gtcgagtgga gctgggacct gctgagcgtt cccgagcggc tgctcgccga acggttggcg
     1921 gtgttcgcgg gcaccattgc gacggccgac gcaatcgcgg tctgcgccga cgaccagctc
     1981 ggcagcgacg acatcgacga tctgctcgac tcactcgttg acaagtcact gctgcaagcc
     2041 agcgcgctcg ccgggacacc gcgctaccgc atgctggaga cgctgcgcga gttcgggcta
     2101 gaacggttga cggcgcacgg tgaggtcggc gacatgcgcc gccgtcacgc cgccaggttc
     2161 tcggaattgg tgcgtgaagc cggtccccat actcgcaccg cgggccagct cccgtggatg
     2221 gctcgtcttg aagacgagcg cgacaacatc ctcgccgcac ttcgtttcct ctgtgaggac
     2281 gaccaggcgc aggccgccct cgaggtcgcc gtcgagatgg ccacgtattg gatgttcagc
     2341 gggcggcacg cagacgcgtc gagttggctc gagttcgcgc tgcaggcgaa aggcgatgct
     2401 gcggtcatta cccgactggg agcagaggcg ctccagcgca tcaactccgt cgcgtcgtca
     2461 ttcacgcggc cacccgacga agtcgagacc agtttggcta gcctgcgcga tctcggtgaa
     2521 cgtatcgacg cagttgagat ccccccggat tcgttcctgt tcatgctgcg cccggtgatc
     2581 gccatgttcg ccgatgacag ggacgcagta gatcggctcg tgaacgaggg cctcgccagc
     2641 accgacgcct gggtcgccgc gagcctgcgc atgtttcggg cggccatggc cgagaacaac
     2701 ggcgacgttg cgacgatgcg gtccgacgtg cagatcgcac tcgcccagtt ccgcgcactc
     2761 ggcgagcgat ggggtcaagc cagctgcctc ggcatcctcg gccaactcaa gacgatggac
     2821 ggcgacctcg acggtgcgat cgcggcgatc gaggaggcga tttctctgag cgaggaactc
     2881 ggcggccacg acgacgcgat catgatgaac attcggttgg ccgacctccg catgcgcacg
     2941 ggcgacatcg acggagctcg cgcctctgtc gcgaagatgc aggagttaac ggacctgtcg
     3001 gcgctcagtc accaggcaat cgtcatctcg ctcgtccggg cagaaatcgc gctcttcgat
     3061 ggcgaccttg accaggcgcg acggctgcgc gacgagtgcc tacgacggat ctcgcaggtg
     3121 tcagaggttc acccggcgca agggcacatc gcagctatcg tgctggcctt cgccgcgagg
     3181 ctcagcttag aagccggcga cctcgacacc gcacgagaca acgtggcgcg ggcgtaccgc
     3241 tacgcgcgcg ggaccaaaga catgcccatc gtcgccatga tcggtgtcac cgctgccgcc
     3301 gccgcgcgcg cagagggtcg gttgaccgat gccgccgagt tgctcggcgc gtcggcttcg
     3361 ttgcgtggcg cagcggacgc gacccaaccc gagatcgcca aactgacagc caggctgacc
     3421 aaggagctag gagcaacggt cttcattgcg gcttacgggt ccgcacgcga cctcgatctc
     3481 gaggccgcgt gcgcacgact ggacgcgatt gtcgctccgc tctagaactc aggcccgcct
     3541 cttgtacgcg cgcaaggcga gcggcacgaa cgtcaccagc aggatcgcca tccagagcag
     3601 cgtccacagc aagtgatagg tgaccgggcc cccgaggaca aggccgcgca ccgtgcccac
     3661 gagatgcgtc aacgggttga ccttgacgaa cccctggagc cacccaggca tggtcgacac
     3721 cttcacgaag acgctgctgc cgaaggagag tggcagcacg agcaggaaca tgatgccctg
     3781 cacggctccc gacgtccggg cgatcatgcc cacgaatacg gagatccagc aaaagcacag
     3841 ggcgaacgcg atggagatcc cgatcgctgc cagcatcgcg agcacgttcg tctggatccg
     3901 gaagccgatg atgtagccaa agccaagtgt catcacgcag acgatcaggt agcgcatgaa
     3961 gtcggccatc acggcgccga cgagcggagc cgatcgagcg ataggcagcg accgaaacct
     4021 gtcgaagacg cccttctcga tgtcggcgtt gaggttttga cccagcgcga cgccggcgag
     4081 cgcgatcgtc tgaccaagca gacccggcaa caggtattcc agatatgcgt gctgcgagcc
     4141 gccgcccaac gcgccgccga acacgtacgt gaagaggagc aggaagatga ccggctgcaa
     4201 cgtcacatcg atgagcgctt ccggcgtccg cgctgtcttg atcaagctgc gtttcgccaa
     4261 cgcgagcgcg tgccgaacgg tgcgccagcg acgagacaat ggctgttctg attcgttgc
//
LOCUS       DASWVF010000848         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_151645, whole genome shotgun sequence.
ACCESSION   DASWVF010000848 DASWVF010000000
VERSION     DASWVF010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151645"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGD55_15030"
     CDS             <1..346
                     /locus_tag="VGD55_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871711.1"
                     /translation="EIGDVLNAGTTPSSSAVPHIGTVPNIGTNTPPTTAALPAGYLIR
                     TAQSPPDPLSLQPVPAAPPHDHAAKEFHLQNAIYVVQWFLLAIIVVVTWWRFLRASRE
                     PHAADRPLEPAV"
     gene            complement(434..985)
                     /locus_tag="VGD55_15035"
     CDS             complement(434..985)
                     /locus_tag="VGD55_15035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0871712.1"
                     /translation="MPKRLVVLLATLFALTGCAGAGGSSKDSVDVVGLTTYAANARHE
                     APALTGKAFDGSALSLSALGAGKVVLLNVWASWCGPCREESPMLGTAAKTYAPEGVVF
                     VGIDEQDTASAGRTFATSAGMTYANFVDPDGALLAKLKMLPQMGIPSTLVLDKHGRIA
                     ARVIGPTNAAELKQVLGNLTAET"
     gene            complement(1620..2324)
                     /locus_tag="VGD55_15040"
     CDS             complement(1620..2324)
                     /locus_tag="VGD55_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0871713.1"
                     /translation="MNLPYDVHAVNIFNVNISAAPPAGPGGAHPYHHGDLRNALVDAA
                     VQLAHEGGPSAIVLREAARRVGVSPNAAYRHFGALPDLVYEVACVALSRMAQVMQVEL
                     DKVVATGDPARDAPARLMAVGRGYVCFALDEPGLFSTAFSAHEEASMQKFSAAVDTPS
                     STTPESLLRDRLDELVAVGIVNPADYDATAKTCWAVVHGLSLLLLGPMSGVPLDERPA
                     LIDACLSVIGRGLAST"
BASE COUNT          434 a          814 c          808 g          416 t
ORIGIN      
        1 cgagattggc gatgttctaa atgctggaac gaccccaagt agcagtgccg tcccacatat
       61 tggaactgtg ccgaatattg gaacaaatac gcctccgacg acggccgcat tacccgccgg
      121 gtacctgatc agaaccgccc aatcgccacc agatccgctc tcgctccagc cggttccagc
      181 cgctcctccg cacgaccacg cggcgaagga gttccacctc cagaacgcga tctacgtcgt
      241 ccagtggttc ctgctcgcga tcatcgtggt ggtgacctgg tggcgattct tgcgagcgag
      301 ccgcgaaccg cacgccgcag accgcccgct cgagccagcc gtctgatcgg tcaggtcccg
      361 gttcgccggt agcgcggtca ggtctcggcg gtcaggtctc ggcggtcagg tctcggcggt
      421 caggtctcgg cggtcaggtc tcggcggtca ggttgccgag cacctgcttg agttcggcgg
      481 cgttggtcgg gccgatgacg cgggccgcta tccggccgtg cttatcgagg acgagcgtgc
      541 tcgggatgcc catctgcggg agcatcttca gtttcgccag caacgcgccg tccgggtcga
      601 cgaagtttgc gtaggtcatg ccggcggacg tcgcgaacgt acgcccggcc gacgcagtgt
      661 cttgctcgtc gatgccgacg aagacgacac cttccggcgc gtaggtcttc gctgcggttc
      721 ccagcatcgg ggactcttcg cggcagggtc cacaccacga ggcccacacg ttaagcaaga
      781 cgaccttgcc ggcgccgagg gctgacagcg atagcgcact gccgtcgaac gccttcccgg
      841 tcagcgccgg agcctcgtgg cgggcgttgg cggcgtacgt cgtaagcccg accacatcga
      901 cggagtcctt cgacgagccg cccgcgcccg cgcagccggt gagcgcgaac aacgtcgcaa
      961 gcagcacgac aaggcgcttg ggcatctcga caggctaacc ccgccgatcg acgcaccaaa
     1021 ccgcgcgcat cgcttagcag tcgcaggcac cttgggtgct gccgtagaac gcacgctgca
     1081 cctgaggacg acgaggccga cggaacacgg cgagcccgtt cgaaaccgac gccgacagcc
     1141 gaccgatctg ttcgtccgca gtgcgttgct cagctggcgt cggtgggcgc gcagcccaat
     1201 acattttgta actgacgtac ggcgtcatga catctctccc atgcgggcgg tttgcgctcc
     1261 gcgcctcgaa ccaacagcat cgccggtggg tacgacaaaa agaggacggc ggcgacgggt
     1321 gcggccgaac cacgggaccc acgaaggcag ccggcgcgga gggcgcagtt cccggcgcgc
     1381 gcgccgcgcg tcctcgatca cgcgcgcgtg ctccaacttc cggatctgtt cagcggcgag
     1441 gctgtggatg agctcgggat acctgtagtc ccgcatggtt tcgtgccttt ctcgttcagc
     1501 gttggcgcgc agcgcagcaa cacgtcgaac tgggtggaca accagtgcac gcggcaacgc
     1561 ggtgtgtcgg ttcgagcgat gacgaatttg tgagaatcgt caccggaacg cggcgtgggc
     1621 taggtcgacg ccaacccacg accgatgacg ctcaggcagg cgtcgatcaa ggcggggcgt
     1681 tcgtcgagcg gcacccctga catggggccg agcaacagca acgaaagtcc gtgcaccacc
     1741 gcccagcacg tcttggcggt cgcgtcgtag tcggcgggat tgacgattcc gactgctact
     1801 agttcgtcaa gacggtcgcg aagcagactc tcgggcgtcg tcgatgacgg cgtatcgacc
     1861 gcggcactga acttctgcat gctggcctct tcgtgggcac tgaacgcggt cgagaaaaga
     1921 cccggttcgt cgagtgcgaa acagacgtag ccgcgaccga ccgccatcaa tcgagcaggg
     1981 gcgtcgcgag ccggatcgcc ggttgcgacc accttgtcga gttccacctg catcacctgg
     2041 gccatccggc tcagcgctac gcacgcgacc tcgtagacga ggtcgggcag ggccccgaag
     2101 tgccggtacg ccgcgttcgg cgacaccccg acccgccgag cggcttcccg gagcacgatc
     2161 gcgctcggcc caccctcgtg agccagttga accgcggcgt cgacgagcgc gttgcgcagg
     2221 tcgccgtggt ggtacgggtg agcgccgccg ggcccagccg gtggagctgc ggaaatgttc
     2281 acattaaaga tgttgacggc gtgaacatca tatggcaagt tcacagtgtc aacatcgccg
     2341 gtcggcgctt aaacctcggg agcctgtcgt gttctacgca ctgggatcag tcattgtcag
     2401 gcgcgccaaa ctcgtgctgg cgctcaccct ggccgtcctg gcgatcttcg ccgtactggg
     2461 cgccggggtc tt
//
LOCUS       DASWVF010000849         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_279275, whole genome shotgun sequence.
ACCESSION   DASWVF010000849 DASWVF010000000
VERSION     DASWVF010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279275"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGD55_15045"
     CDS             <1..300
                     /locus_tag="VGD55_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0871714.1"
                     /translation="LLREPPATLVSAIIDTDIAPLRPDVSLPAITSHLATYNLVAAPV
                     VDDDDRLLGAVTVDDVLDHLLPDGWRDTDPSTETTAEPTPEPTPEPTQGVPNGAP"
     gene            287..808
                     /locus_tag="VGD55_15050"
     CDS             287..808
                     /locus_tag="VGD55_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0871715.1"
                     /translation="MARREPQRARLDQPKGDRRVTLRPDYDPEAFGKLSERFARFLGT
                     AKFIIWMSVFIIIWLVWNTWGPKQERFDPHSLNYTLLTLLLSLQASYAAPLILLAQNR
                     QADRDRVQSEDDRSRNERNIADTEYLAREVASLRIALGDVATRDFIRGELQRMLEDLD
                     ERKHPPPDERDAV"
     gene            complement(824..>2075)
                     /gene="glgC"
                     /locus_tag="VGD55_15055"
     CDS             complement(824..>2075)
                     /gene="glgC"
                     /locus_tag="VGD55_15055"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY0871716.1"
                     /translation="PHMANGRKVLGIVLAGGQGTRLLPLTADRAKPAVPFGGIYRLID
                     FVLSNLVNGGFLKIVVLTQYKNHSLDRHITTTWRMSTLLGNYVTPVPAQQRVGPRWFA
                     GSADAIYQSLNLVYDERPDYILVFGADHIYRMDPRQMLDLHISSGASATVAGIRAPRK
                     EASAFGVIQTAQTGPDAGRRIEAFLEKPQDIEGIGLPDSPDEVFASMGNYCFTTDALL
                     DAVNSDAADERSLHDMGGNIIPMFVDRGEAQVYDFDDNIVPGATERDRAYWRDVGSLD
                     AYYDAHMDLISVHPIFNLYNENWPIYTNHRPLPPAKIVHEETRVIDSMLSNGVIVADS
                     MVAGSVLSPNVHIDSRADIDGSVLLENVRVGQGAVIRNAIIDKNVVIPAGAKIGVDLA
                     RDRQRFTVSDHGIVVIGKNAVVEV"
BASE COUNT          374 a          695 c          648 g          358 t
ORIGIN      
        1 ttgttgcgcg agccgcccgc gacgctggtg agcgcgatca tcgacaccga catcgccccg
       61 ctgcgtcccg acgtgtcgtt gcctgcgatc accagccatc tggcgaccta caacctggtc
      121 gcggcacccg tcgtcgatga cgacgaccgc ctgctcggcg cagtcacggt cgacgacgtt
      181 ctcgaccact tgctgcctga cggctggcgc gacaccgacc cgagcaccga aacaaccgcg
      241 gaaccaaccc ccgaaccaac ccccgaacca acgcagggtg tgcccaatgg cgcgccgtga
      301 gccacagcgt gcgcggctcg accagccgaa aggcgaccgc cgggtaacgc tgcggcccga
      361 ctacgacccc gaggcgttcg gcaagctgtc ggagcggttc gcgcggttct tgggcacggc
      421 gaagttcatc atctggatgt cggtcttcat catcatctgg ctcgtctgga acacctgggg
      481 tcccaaacag gagcggttcg acccgcacag cttgaactac acgctgctca cgttgttgct
      541 ttccctccag gcgtcctacg ctgcgccgct gatcctgcta gcgcaaaacc ggcaggccga
      601 ccgcgaccgg gtgcagtcgg aagacgaccg ctcccgcaac gagcgcaaca tcgctgacac
      661 cgagtatctc gcccgcgagg tcgcgtccct gcggatcgcg ttgggtgacg tggcgacccg
      721 cgacttcatc cgcggcgaac tccagcggat gctcgaggac ctcgacgagc gcaagcaccc
      781 gccgcccgac gaacgcgacg ccgtctaaaa cgcgcgcgct gccctagacc tcgacgaccg
      841 cgttctttcc gatcacgacg atgccgtggt ctgacaccgt gaatcgctgg cggtcccggg
      901 cgaggtctac gccgatcttc gcgccagccg ggatcaccac gttcttgtcg atgatggcgt
      961 tacggatcac ggcaccttga cccacgcgaa cgttctcgag caagacactg ccgtcgatgt
     1021 cggcccggct gtcgatgtgc acgttgggcg agagcaccga acccgcgacc atcgagtcag
     1081 cgacgatcac gccgttcgac aacatggagt cgatgacccg cgtctcctcg tgcacgatct
     1141 tggccggcgg caacggcctg tggttcgtgt agatcggcca attttcgttg tagagattga
     1201 aaatcgggtg cacagagatg aggtccatgt gcgcgtcgta gtacgcgtcg aggctcccta
     1261 cgtcacgcca gtaggcgcgg tcacgctcgg tcgcaccggg cacgatgttg tcgtcgaagt
     1321 cgtagacctg ggcctcgccg cggtcgacga acatcgggat gatgttgccg cccatgtcgt
     1381 gcagagaccg ctcgtctgcc gcatcgctgt tcactgcgtc gagaagggcg tctgtcgtga
     1441 agcagtagtt gcccatcgaa gcgaacacct cgtcgggcga atccggtagc ccgatgccct
     1501 cgatgtcctg cggcttttcc aagaacgcct cgatgcggcg tccggcatct ggcccggtct
     1561 gggccgtctg aataaccccg aacgccgagg cctccttgcg aggagctcga atgccggcga
     1621 ccgtcgcgct cgcgcccgag gagatgtgca ggtcgagcat ttggcgcgga tccatccggt
     1681 aaatgtggtc agcgccgaac accaggatgt agtcgggccg ctcgtcatac acgaggttca
     1741 agctctgata gatcgcatca gcggaccctg cgaaccaacg cggaccaacg cgctgctgag
     1801 ccggcaccgg cgtcacgtag ttgccgagca gggtcgacat ccgccaggtc gtggtgatgt
     1861 gccggtcgag gctgtggttc ttgtactggg tcaagacgac gatcttcagg aagccgccgt
     1921 tcaccaggtt cgacagcacg aagtcgatga gccggtagat gcccccgaac gggacggcgg
     1981 gcttcgcccg gtcggcggtg agtggcagca ggcgagtgcc ctgcccacct gccagaacga
     2041 tccccagaac cttgcgcccg ttcgccatgt gcgga
//
LOCUS       DASWVF010000850         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_406849, whole genome shotgun sequence.
ACCESSION   DASWVF010000850 DASWVF010000000
VERSION     DASWVF010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406849"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VGD55_15060"
     CDS             <1..764
                     /locus_tag="VGD55_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY0871717.1"
                     /translation="RRLESLRLRLHPLHEADTSRRAVGLYAGLGVFVSCVVLEMIGAA
                     SATIGKSTDALNNPTSAFTGHMSTALADFVLVAIAIGAVCANVLNIYSGAMSFLTLGF
                     RLPLALRRAIVAAVAGTIGFIVALTGLHDAGEKYTNFLLVVAYWIAPWLGVMFSDMLL
                     RRRVRVDGFLFDTKHNPWAGWVAMGVAMVVSIWLFSDQTKYLAPIPKAHPGLGDLTFE
                     VGFVLAAVLYAVFYKLQGGRKSETLVIPEYGAGKK"
     gene            complement(784..>1684)
                     /locus_tag="VGD55_15065"
     CDS             complement(784..>1684)
                     /locus_tag="VGD55_15065"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0871718.1"
                     /translation="LTALRALIEIREEARGLIDLQLVAFPQLGIMSFDGGRELMRKAV
                     ELGADVVGGIPHYELTREDGVESVQFSFALAEEFGLRVDIHCDETDDDHSRFVEVMAA
                     ETIRRGMSGRVTASHTTAMHSYNAAYAYRLVNNIKRAGMHMVTNPLDNAVLQGRFDTG
                     PIRRGHTRVKQLQEAGVNVCIGHDSVMDPWYPLGYGDPVQAAFVFVHLGHYSGDDELR
                     RLLDMVTVNPAAALGIEGYGIAEGTAADFVVFDAPTDTDAIRLGSRRLLVVRAGRVVA
                     RTAPAQTTVTWDGEEQPVTFLRP"
BASE COUNT          269 a          544 c          563 g          308 t
ORIGIN      
        1 gccgtcggct ggaatccctt cgcctccgac tacacccgct acatgaagcc gacacgtctc
       61 ggcgggcggt cgggttgtac gcgggcttgg gcgtgttcgt gtcgtgcgtg gtactcgaaa
      121 tgatcggcgc ggcgtcggcc acgatcggca agtcgaccga cgcgctgaac aacccgacga
      181 gcgcgttcac cgggcacatg agcacagcgc tggccgactt cgtgctggtc gcgatcgcga
      241 tcggtgcggt atgcgccaac gtgctgaaca tctactcggg cgcgatgtcg ttcctgactc
      301 ttggattcag gctgccgctt gctttgcgcc gtgccatcgt tgcggcggtc gctggcacca
      361 tcggcttcat cgtcgcgctg accggcttgc acgacgccgg cgagaagtac accaacttcc
      421 tgctcgtcgt cgcgtactgg atcgcgccgt ggttgggcgt gatgttctcc gacatgctgc
      481 tgcgccggcg ggtgcgagtc gacggattcc tcttcgacac caagcacaac ccgtgggccg
      541 gctgggtcgc gatgggcgtc gcgatggtcg tgagtatctg gctgttctcc gaccagacca
      601 agtacctcgc gccgatcccg aaggcgcacc ccgggttagg cgacctcacc ttcgaagtcg
      661 gcttcgtgct cgcggcggtt ctgtacgcgg tcttctacaa gctgcagggc ggccggaagt
      721 cagagacgct ggtcatcccc gaatacggtg ccggcaagaa gtaggccgcc agccgcgggc
      781 acgctacggg cggaggaagg tcactggttg ctcttcgcca tcccaggtca ccgttgtttg
      841 cgctggagcg gttcgagcaa cgacgcgacc agcccggacg acgagaagcc ggcgtgatcc
      901 gagccggatc gcgtcggtgt cggtgggcgc atcgaacacc acgaaatcgg ctgccgttcc
      961 ttcggcgatg ccgtaaccct cgatacccag ggcggccgcg gggttgacgg tcaccatgtc
     1021 gaggagccgg cgcaactcgt cgtcgccgct gtagtggccc aggtgcacga agacgaacgc
     1081 ggcctgcacc ggatcgccgt atccgagcgg gtaccacggg tccatcaccg agtcgtggcc
     1141 gatgcagacg ttgacgccgg cttcctgaag ttgcttgacg cgggtgtggc cgcggcggat
     1201 cggtccggtg tcgaagcgtc cctgcagcac cgcgttatcg agcgggttcg tcaccatgtg
     1261 catgccggcg cgcttgatgt tgttgaccag ccggtaggcg taagcggcgt tgtacgagtg
     1321 catcgcagtc gtatggctag cggtcacccg ccccgacatc ccgcggcgaa tcgtctcggc
     1381 ggccatcacc tcgacgaaac gcgagtggtc gtcgtctgtc tcgtcgcagt ggatgtcgac
     1441 gcgcagcccg aattcctcgg ctagcgcaaa ggagaactgc accgactcga cgccgtcttc
     1501 gcgggtgagc tcgtagtgcg gaatgccgcc aacgacgtcg gcaccgagct cgacggcctt
     1561 gcgcatcagc tcgcggccgc cgtcgaacga catgatgccc agctgcggga acgccaccaa
     1621 ctgaaggtcg atcaggccgc gcgcttcttc gcggatctcg atcagcgcgc gcaacgcggt
     1681 cagc
//
LOCUS       DASWVF010000851         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_126220, whole genome shotgun sequence.
ACCESSION   DASWVF010000851 DASWVF010000000
VERSION     DASWVF010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126220"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VGD55_15070"
     CDS             complement(<1..995)
                     /locus_tag="VGD55_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEY0871719.1"
                     /translation="MSSNQGESVFARLEPLLMSVSKPVQYVGGELNSLVKDWAACDVR
                     WCLMYPDAYEVGLPNQGVQILYEVLGELDGVLVERTYSVWPDLEALMREHGIPQFTVD
                     AHRPVGAFDVFGISFSTELGYTNMLNALDLAGIPIESADRTDEHPLVIAGGHAAFNPE
                     PIADFIDAAVHGDGEQAVIAITHIVREWKREGSPGGRDEVLYRLALTGNIYVPRFYDV
                     SYRADGHIDAVTPNRVGVPARVSKHTLMDLDAWPYPKKPLVPLAETVHERFSVEIFRG
                     CTRGCRFCQAGMITRPVRERSIDTIGAMVDAGLKASGFQEVGLLSLSSADHSEIGP"
     gene            complement(1011..>1839)
                     /locus_tag="VGD55_15075"
     CDS             complement(1011..>1839)
                     /locus_tag="VGD55_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0871720.1"
                     /translation="GGGLEIQPSEFAKLAVIVLVAMLFSSAREDRRDITDLDVARALV
                     LMGIPMLLVLLQNDLGTMLVMVAIVFGVLAIGGVPTRWMIGLTFAMFAATIVIVQLHL
                     LSTYQVNRITAYLHPNSPSVLRDSGFNAHQAQLAIGAGGLTGTGLFHGSQINSGAVYA
                     AHTDFIFATAGEELGFLGSALIIVLLSVILWRGLRIAARAPDLFGRIVAGGVVCWLAF
                     ETFENIGMNLGIMPVTGIPLQFVSYGGSSLFASMLAVGLLQNVHIQTQATQNSSGGL"
BASE COUNT          394 a          571 c          567 g          307 t
ORIGIN      
        1 gggccgatct cggaatggtc ggcgctgctc agtgatagca gcccaacttc ttggaagccg
       61 ctcgccttca acccggcatc gaccatggcg ccgatcgtgt cgatcgaccg ttcgcgcaca
      121 ggccgggtga tcatgcctgc ctgacagaag cgacagcccc gtgtgcagcc gcggaagatc
      181 tcgacactga aacgctcatg cacggtttct gccagtggta ccaacggctt cttcgggtac
      241 ggccatgcat cgagatccat cagcgtgtgc ttcgaaacac gcgccggaac gccgacccga
      301 ttcggggtca cggcgtcgat atgaccatcg gcgcgataac tcacgtcgta gaagcggggt
      361 acgtagatgt tgccggtgag cgccaatcgg tacagcactt catcgcgccc accgggagag
      421 ccttcacgct tccattcgcg gacgatgtgc gtaatggcga tgaccgcctg ctcaccgtca
      481 ccatgcactg cggcatcgat gaaatctgcg atcggctcag gattgaaagc tgcgtgaccg
      541 ccggcgatca ccagaggatg ttcgtcggtg cgatcagccg actcgatcgg gataccggca
      601 aggtcgagtg cgttgagcat gttcgtgtag ccgagctcgg tcgaaaacga gatgccgaat
      661 acgtcgaacg cgccgaccgg ccggtgcgca tcgacagtga actgcgggat gccgtgttca
      721 cgcatcaggg cttcgagatc gggccacacc gaataggtcc gttcgacgag tacgccatcg
      781 agttcaccga gcacctcata gaggatttgc acgccctggt tgggcaggcc aacctcgtag
      841 gcgtcggggt acatcagaca ccaccggacg tcgcaggcag cccaatcttt gacgagtgag
      901 ttcagttcgc cgccgacgta ttggacgggc ttgctgacgc tcatcagcag cggctcgagc
      961 cgggcgaaca ccgactcgcc ctggttgctc gacaccactg aagactagct ctaaaggccg
     1021 ccgctggagt tctgggtcgc ctgcgtctgg atatgtacgt tctggagcag cccgaccgca
     1081 agcatgctcg cgaacaggga cgacccgccg taagagacga actgcagcgg aattccggtg
     1141 acgggcatga tcccgagatt catcccgatg ttctcgaagg tctcgaaggc cagccagcag
     1201 accaccccgc cagcgacgat ccgcccgaac aggtcgggcg cccgggccgc aattcggagc
     1261 ccgcgccaca ggatgacgga caacaacacg atgatcagcg ccgaaccgag aaagccgagc
     1321 tcctcccccg cggtcgcgaa gatgaagtcg gtgtgcgccg cgtagaccgc accgctgttg
     1381 atttgggaac cgtggaacag cccggtaccg gtgagcccgc cggcgccgat ggcgagctgc
     1441 gcctggtgcg cgttgaaccc cgaatctcgc aatacggacg gcgagttcgg gtgcaggtag
     1501 gcggtaatac ggttcacctg gtaggtgctc aaaagatgaa gctggacgat cacaatcgtc
     1561 gccgcaaaca tcgcgaaggt gaggccgatc atccaacgcg tcggcacccc gccgatggca
     1621 agcacgccga acacgatcgc gaccatgacg agcatggtgc cgaggtcgtt ctgtaggagc
     1681 accagcaaca tcggaatacc cataagcacc agggctcttg cgacatcgag gtcagtgatg
     1741 tcgcgacgat cctcacgagc agaactgaac aacatcgcga ccagaacgat gaccgcaagc
     1801 ttggcgaact ccgacggctg gatctcgagg ccgcctccg
//
LOCUS       DASWVF010000852         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_126294, whole genome shotgun sequence.
ACCESSION   DASWVF010000852 DASWVF010000000
VERSION     DASWVF010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126294"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VGD55_15080"
     CDS             complement(<1..1043)
                     /locus_tag="VGD55_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0871721.1"
                     /translation="MKHQRLVALLAVAAVATLAGCSSSKPKASSASSAAPSVSSAAST
                     AATATSSAPVATSAAPASSAPATDVSAPTSITVNCEPPTTAAALRTQWLDDVASFEKL
                     HPQITINSKDEAPCDDPNTFSSKLASGQEENVFYLYLTDTQQAIDSGQALDIQSYAAD
                     IPGLSDIQTSVLNVFKKGGATGGDLYGLPRQNYTLGLVYNRALFTAAGLDPNSPPTTW
                     ADVETDAKKIAALGNGDVGYADYSAGNTGGWHFTAEMYSQGGDVVDSTGKKADFDNAK
                     GLAVINNLKQMRWTDNDMGSKQLLQYQDTINMMAAGHLGMTIGAPDYLTNMQQTDKAD
                     LTGFGMGAMPAGA"
     gene            complement(1347..>1697)
                     /locus_tag="VGD55_15085"
     CDS             complement(1347..>1697)
                     /locus_tag="VGD55_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY0871722.1"
                     /translation="DGNTGSYWESTNHAFPQSITVDLGATESIRKLVLTLPPDSAWTT
                     RVQTITVLASTDGSTFSTVLPSATYTFDPTTKNTVTVSLPTASTVMARYVRLAVTANT
                     GWPAAQFSEIEVFP"
BASE COUNT          274 a          507 c          572 g          344 t
ORIGIN      
        1 ctggcgccag ccggcatcgc gcccatgcca aagccggtga ggtcggcctt gtcggtctgc
       61 tgcatgttgg tcaggtagtc cggcgcacca atcgtcatac cgaggtgacc ggccgccatc
      121 atgttgatgg tgtcctggta ctggagcagc tgcttggagc ccatgtcgtt gtcggtccag
      181 cgcatctgct tgagattgtt gatgaccgcc aggcccttcg cattgtcgaa gtccgccttc
      241 ttgccggtgg agtcgacgac gtcgccgcct tgtgagtaca tctcggcggt gaagtgccag
      301 ccaccggtgt tgccggcgct gtagtcggca tagccgacat cgccgttacc gagcgccgca
      361 atcttcttcg cgtcggtttc cacgtcagcc cacgtggtcg gagggctgtt cgggtcgagc
      421 ccggcggcag tgaacaacgc gcggttgtag accaggccga gcgtgtagtt ttgcctcggc
      481 aggccgtaga ggtcgccgcc agtagcgccg cccttcttga acacgttcaa gaccgacgtc
      541 tggatgtcgg aaaggcctgg aatgtccgcg gcgtacgact ggatatcaag cgcctgaccg
      601 gagtcgatcg cctgctgggt gtcggtcagg tagaggtaga acacgttctc ttcctggccg
      661 ctggccagct tcgacgagaa ggtgttcggg tcgtcgcacg gagcttcgtc cttgctgttg
      721 atcgtgatct gcgggtgcag cttctcaaac gatgccacgt cgtcgagcca ctgcgtgcgc
      781 agggcagcag cggtggtcgg cggctcgcag ttgacggtga tgctcgttgg cgcactgacg
      841 tcggtcgccg gcgctgaact cgcgggcgcc gcactggtcg ccacaggtgc actagacgtg
      901 gctgtggccg cggtgctcgc cgcacttgac acgctcggag ccgcgctgct tgccgagctc
      961 gctttcggct tgctgctcga acaaccggcc aaggtggcca ctgccgcgac tgcgagcagc
     1021 gccaccagtc tctggtgctt catcgaacgt tcctcctgga aagtcggtcc ggggcgtcgc
     1081 tcgccgcggc agtcacgcag gtcgctcttg ccggtgaccg agtcatctgc tgagagatcc
     1141 ggcgttcgag cccggctgcc acgggagcga cgcgacgggt gcacgccgtc ggaccttcca
     1201 tggcagccga gttgcactga aggtacagat gccgacgcat gtgagcaata gattgactga
     1261 ctgttgcaaa atcgtgacgt aacactgaag gaccttcatc gaatcggtcg aagccgcctc
     1321 atcgcccgca catcggacag cagttgttac gggaagacct cgatctcgga gaactgagcc
     1381 gccggccagc ccgtgttggc agtcacggcg aggcgcacgt agcgggccat gactgttgag
     1441 gcggtcggga gcgacaccgt gacggtgttc ttcgtggtgg ggtcgaacgt gtacgtcgcg
     1501 ctgggcaaca cggtcgagaa cgtgctccca tcggtactgg caagcacggt gatggtctga
     1561 acacgggtgg tccatgccga gtcgggcggc aacgtgagca ctagcttgcg gatcgactcg
     1621 gtggcgccga gatcgaccgt tattgactgt gggaacgcat ggttcgtgct ctcccagtag
     1681 ctgccggtgt tgccgtc
//
LOCUS       DASWVF010000853         8975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_483557, whole genome shotgun sequence.
ACCESSION   DASWVF010000853 DASWVF010000000
VERSION     DASWVF010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483557"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGD55_15090"
     CDS             complement(<1..210)
                     /locus_tag="VGD55_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0871723.1"
                     /translation="MAVIRAALVQTSWTGDKESMIQAHEEYARKAAAQGAQAICFQEL
                     FYGPYFCQVQDASFYSYAEAIPGPTT"
     gene            354..1952
                     /locus_tag="VGD55_15095"
     CDS             354..1952
                     /locus_tag="VGD55_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0871724.1"
                     /translation="MDDAVPARGPTSATTATTQTRVRLADLVAALSLGVDLGFGQPME
                     HVLRQCLIALRVADRIGLDDEQRSVVYYTALLVNVGCHADAHEQAKWFGDDIELKSHK
                     YDHEFRSVRGALASLRLVGSGNPPLHRFRVGLEFAVSGHRDLDGMIEQHARLACSLAR
                     EIGLPDAVQDAVGASYEQWDGRGWPGNLAGEQVPIAARLAQLAEFVEVAHRVGGIDAA
                     VTLARKRSGSQFDPALAALICSDAREILGGLDATRTWATVIGAEPALGFTLAGPQLDA
                     ALVGVANFVDLKSPYTLGHARAVCELVGVAGAYLGLTADEVAALRHAGLVHGLGRLGV
                     SNSIWDKRGPLAAGEWERVRLHPYLTERMLRQSSWLAPLGEIAVQLRERLDGSGYPRG
                     LTGGAISTSARILGAADAYQAMREPRPYRDALSADDAASQLRREVHDGRMDADAVEAV
                     LSADGHRVLRRREGPAGLTAREVDVLRLLAQGLSSREIAARLVIAPKTARNHIEHIYA
                     KIGASSRATASLFAMQHGLLPGHE"
     gene            2030..2413
                     /locus_tag="VGD55_15100"
     CDS             2030..2413
                     /locus_tag="VGD55_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871725.1"
                     /translation="MPKVSKVTASQVQDFGAAVDHAEHLDDYTVDFVSIRESHSLAPL
                     LKGLPDDACQCPHWGYLFTGTLTVSYTDRPDETFHGGDAFYMSPGHVPSAEAGSEFVQ
                     FSPKDQLAHSQAAMMANAQRMATRS"
     gene            2423..3208
                     /locus_tag="VGD55_15105"
     CDS             2423..3208
                     /locus_tag="VGD55_15105"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0871726.1"
                     /translation="MMGAVSGTHPMRHLYGPDPSQFADLYLPAGQPRPITVVLVHGGW
                     WGPHYGLDHLEQEAVDLARRGWVTWNIEYRRIGIGGGYPSTLEDAAAAVDYLATMNNV
                     STDQVVTMGHSAGGHLATWAAGRSKLAVGAPGAKPVVEIAGVISLAGVVDLGAAARDR
                     SGNSAAIKLMGGRPDEHPDWYAVADPLTRVPIPAVVRCVHARADDEVPFSESVTYVAA
                     AQAAGQDASLIEASGDHYSVADATSADWPAIIDALEELETLLA"
     gene            complement(3236..3898)
                     /locus_tag="VGD55_15110"
     CDS             complement(3236..3898)
                     /locus_tag="VGD55_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871727.1"
                     /translation="MQVRAVPAAAAVVVLALSLAACNPATKSKATITPTQVPVATETQ
                     ADEASAANSTASHSTASSAPASASTASSTPVVSTSPPSGPGPAAGGATIDACSLLTSA
                     QASAINSVTYGAATSGHLVAGIDTCTYANTGKHANELDIQDLEVEVISLPGCYSELQE
                     AQGPGTKVPGVGDDAFGYEIGIDVKLGNRCVQISGLTDAEFRDNYAPDVAMAKIIISK
                     LS"
     gene            4088..6874
                     /locus_tag="VGD55_15115"
     CDS             4088..6874
                     /locus_tag="VGD55_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0871728.1"
                     /translation="MRTDPPDLIGRASERAAILGALHASANGPAAVLLTGDAGIGKTA
                     IWESIVAEQRAAGDHVLVSRATAAEARLPWVGLTDLMRTIPPEALASLPDVQRQALEA
                     VSLQAVSLQRGSSDALDERTVGTALLSALQAVATTAPVLLAIDDLPYFDTASASAVTF
                     ALRRMEGRHRTRLLATIRDHDLRLVALQGLPRDRYSVISVGPLTLGALFDLLQTRRGI
                     RLPRPLLLRVYETSGGNPLYALELARALDRLEINPKPGIPLPVPDGLNALVDARVRDL
                     PTEVAEVLAATAAAWRVNADEQDAEAVELAVAAGMVVIAEPVVVGGARVIRSTHPLLS
                     AAAYNGLSTSRRRKLHARLAAAADDPVERVRHAALAANEPQAALALDLDVGVAAALAA
                     GVPDIAVGLAQLSLEHTTDIDLRPVRLDRLADAQLRSGDSSGAWQTQSEAIAATAEGA
                     ERARRRIRLAEIAVEVTSWADAERELQVAIAEATDNPVVLAEALLTLAAVTDDIDLAE
                     TSAQRAVDILLTQDDPDPMILSGALSQLAGARFRAGRGLDHELFARAIEIERAHPSRR
                     LSDRADASYAALLKYADDLDGAEGLLTSLLKEARATGDLSSITYAISHLVHLYLWRGE
                     LARGRSCADEHLEVAVHGELQGQEDQARYNVGLAMACQGQLDDADRLLLGLLETVTAS
                     THAWLVHRVHAVLGFIALSRNDAVTAAQHTDLWHDALIEMHFGEPGYSRSHLDHLCAL
                     VDTGRTADADAFCLELDLQAKRSGRASAAAVVLTGRAMLEAHAGRLSDAVALVASALS
                     WYDTSPLRFDRARTLLIAGRISRRAKAKSDAHALLTEAEHEFASFGATAWQAQAAAEL
                     ARVNVRPAAPAELTETERLVAQLAASGLTNREVADRTFLAVKTVEANLARVYRKLGIR
                     SRAELGARMGANAS"
     gene            complement(6887..7132)
                     /locus_tag="VGD55_15120"
     CDS             complement(6887..7132)
                     /locus_tag="VGD55_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871729.1"
                     /translation="MVGFLVERYWPSVTAADVEAVGRRLTQLSTQDATFVASTLMPTD
                     EVVFFEFMAVDEAAVLDLARLADLRCDRLMFAERNGG"
     gene            7266..8042
                     /locus_tag="VGD55_15125"
     CDS             7266..8042
                     /locus_tag="VGD55_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871730.1"
                     /translation="MTASPALTRLALLAATALLLAGCNSGSSGKASSALIPSSAPAVD
                     SASAAPSAGDASDAPLPVASGHPAVTCQQLTLQQVQPFIGEKLTGDAVQFENLGDVTG
                     QQCTFSGASDEHSINILVLKGSGAAAAYSSEVADEADGQVDVPEVGDKASRDKGSGGI
                     NALKGNVYCSTGVDDGLPAIGTLESAANGTTNIGEAYYQEAAIAVGTLCNRIFGSGNT
                     TPDLSKLLAAAAVASASAAATASAGEAAQSAFASTYAPAG"
     gene            complement(8062..8394)
                     /locus_tag="VGD55_15130"
     CDS             complement(8062..8394)
                     /locus_tag="VGD55_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871731.1"
                     /translation="MTYGATTPQQVEKGFDSCTYKNNGSKNPVDIQDLTVQVISLPDC
                     YTQLQQADGPGTPVSGVGDAAFGYSIGIIVKVGSRCLEVSGLTGAELQNNYAPDVAMA
                     KIIISKLS"
     gene            8423..8719
                     /locus_tag="VGD55_15135"
     CDS             8423..8719
                     /locus_tag="VGD55_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871732.1"
                     /translation="MRSPQTSTVPPPDAGGGVTVAAALAGPADALEPTDGLAVELVGD
                     VGDVGAAAVAVAGTPVPTAVAFAFVAGLHAVEHNATTATAAAAESARTFIAAPC"
BASE COUNT         1526 a         2972 c         2960 g         1517 t
ORIGIN      
        1 ggtggtcgga cccgggatcg cctcggcgta gctgtagaaa gaggcgtcct gaacctggca
       61 gaaataggga ccgtagaaga gctcctggaa gcaaatcgcc tgggcgccct gcgctgcggc
      121 cttgcgggcg tactcctcgt gcgcctggat catcgactcc ttgtcgccgg tccagctcgt
      181 ttgcacaaga gccgcgcgaa taaccgccat cgccaccgcc tccgatcagc ccgtcaacag
      241 aagggaacag actaagggtc acatctaggg acgggccatg acctccctgg gtggggctgc
      301 tgcagcgtag agtcggtcgc gcccgcgacg tagctgtcgg tttgggggct tcaatggacg
      361 acgcggtgcc cgcacggggt ccgacgagcg cgaccactgc gaccacacag acgcgtgtgc
      421 ggctcgccga cctggtcgct gcgctttcgc tcggggtcga cctgggcttc ggccaaccga
      481 tggagcatgt gctgcggcaa tgcctaatcg cactccgcgt tgccgaccgg atcgggctcg
      541 atgacgagca acgatcggtg gtctactaca cggcgctcct cgtcaatgtg gggtgccatg
      601 cggacgcgca cgagcaggcg aaatggttcg gcgacgacat cgagctcaaa tcacataagt
      661 acgaccacga gttccgcagc gtccgcggcg cactcgcgtc actgcggctc gtcggcagcg
      721 gcaatccgcc gctccaccgc ttccgcgtcg gactggaatt cgcggtctct ggccaccgcg
      781 acctcgacgg catgatcgaa caacacgctc ggctcgcttg ctcgctcgcc cgggagatcg
      841 gcctgcctga cgcggttcaa gacgcggtcg gtgcgtcata cgagcagtgg gacggacgtg
      901 gctggccggg caatctcgcc ggggaacagg tacccatcgc tgcccggctg gcccagctgg
      961 ccgagttcgt cgaagtcgcc caccgcgtcg gtggaatcga cgcagcagtg acgctcgccc
     1021 gcaagcgaag cggctcgcaa ttcgaccccg cgcttgcggc ccttatctgc tcggacgcgc
     1081 gcgagattct cggcggcctc gacgcgacac gaacttgggc caccgtcatc ggtgcggagc
     1141 cggcgctcgg cttcactctg gccggaccgc agctcgacgc ggcactggtc ggtgtcgcca
     1201 acttcgtcga cctcaagtcg ccctacaccc tgggccacgc ccgtgcagtc tgcgagctcg
     1261 tcggggtcgc cggtgcctat ctcggcctga ccgctgacga ggtcgcagcg ttgcggcacg
     1321 cagggctcgt gcacgggcta ggtcgtctcg gcgtttcgaa ctcgatttgg gacaagcgcg
     1381 ggccattggc cgccggggaa tgggagcggg tgcggctgca cccctatctg actgagcgca
     1441 tgctccgtca gtcgagttgg cttgcaccgc tgggcgagat cgccgtgcaa cttcgcgagc
     1501 gacttgacgg ctccggatac ccgcgcgggc tcacgggcgg tgcgatctcg acgtccgctc
     1561 gaattcttgg cgcggccgac gcgtaccagg cgatgcgtga gccgaggccg tatcgcgacg
     1621 ctttatctgc ggacgacgca gcgtcgcagc tgaggcgtga agtgcatgac ggccgcatgg
     1681 acgccgatgc cgtcgaagcc gtgttatcgg ccgacgggca ccgggtgctg cggcggcgcg
     1741 agggtccggc cggattgacc gcgcgcgagg ttgacgtgct gcgactgctc gctcagggcc
     1801 tgtcgagccg ggagatcgcg gcgcgactgg tcatcgcgcc gaagacggca cgcaatcaca
     1861 tcgagcacat ctacgcgaag atcggcgcct caagccgtgc gacggcgagc ctgttcgcta
     1921 tgcagcacgg cctgctcccc gggcacgagt aggtcgcctc cccgagcgcg ctcaaatggg
     1981 gcagttgccc catgcgcgga agctgagctc ccgcctaggg tcactggcta tgccgaaggt
     2041 ctcgaaggtc actgcatcac aggttcagga cttcggtgct gccgtggatc acgcggagca
     2101 tctcgacgac tacaccgtcg atttcgtctc gatccgtgaa tcacactcac tcgcaccgct
     2161 gctcaaggga ttgccggacg acgcgtgcca gtgtccccac tggggctatc tcttcaccgg
     2221 cacactaacg gtgtcataca cagaccggcc cgacgagaca ttccacggtg gcgacgcgtt
     2281 ctacatgagc cctggccacg tgccgtcagc cgaagccggc agcgagttcg tccagttcag
     2341 tcccaaggac caactggcgc acagtcaagc ggcgatgatg gccaacgcgc agcggatggc
     2401 gacccgcagc tagcagcagc ggatgatggg tgccgtgagc ggcacccatc cgatgcggca
     2461 tctctacggc cccgaccctt cgcagttcgc tgacctctac ctgcccgccg gacaacctcg
     2521 accgatcacc gtggtgctgg tccacggagg ttggtgggga cctcactacg ggctcgacca
     2581 cctggagcag gaagcggtcg accttgcgcg acgcggctgg gtgacgtgga acatcgagta
     2641 ccgacgaatc gggatcggcg ggggttatcc gtcaacactg gaggacgcgg cagcagcggt
     2701 cgactatctc gcgaccatga acaacgtcag cactgatcag gtcgtgacga tgggccacag
     2761 cgctggcggt catctcgcga cgtgggcggc cggccgatcc aagcttgcag tcggcgcacc
     2821 cggtgctaaa ccagtcgtcg agatcgcagg cgtcatcagc ctcgcgggcg tcgtcgacct
     2881 cggcgccgca gcccgagaca ggagtggtaa cagcgcagcg atcaagttga tgggcggccg
     2941 cccagacgag caccccgact ggtacgcagt ggccgacccg ctcactcgtg tgcccatccc
     3001 cgccgtcgtt cgctgcgtgc atgcgagggc cgacgatgaa gtcccgttca gcgagagcgt
     3061 cacgtacgtc gcggccgcac aggcagcagg ccaggacgcg tcgttgatcg aggccagcgg
     3121 agatcactac tcggtagccg acgcaacgtc tgctgactgg ccggcaatca tcgacgccct
     3181 cgaggaactc gagacacttc tcgcgtgaca tagctgaacg cagtcgcgtg gtcgttcagg
     3241 aaagcttcga gatgatgatc ttggccatcg caacatcggg tgcgtagttg tcacggaatt
     3301 cggcgtcggt caggcccgag atctgaacgc accggttgcc gagcttgacg tcgatgccga
     3361 tctcgtatcc gaacgcgtcg tcgccgacgc cggggacctt ggtgccgggg ccctgcgcct
     3421 cctgcagttc gctgtagcac cccgggaggg agatcacttc gacctccaga tcctgaatgt
     3481 ctaactcgtt ggcgtgcttg ccggtgttcg cgtaggtgca ggtgtcgatg cccgccacca
     3541 ggtgcccgga cgtggcggct ccgtacgtca cgctgttgat cgccgaggct tgagccgatg
     3601 tcaagagcga gcaagcgtcg atggttgcgc cccccgcagc gggtccgggc ccagacggcg
     3661 gagatgtcga caccactggc gtgctggacg cggtgctcgc acttgctggc gcgctcgacg
     3721 cggtcgagtg gctagcagtc gagttcgcgg cggacgcctc gtctgcctgg gtctcggtcg
     3781 cgaccggaac ctgcgtcgga gtgatcgttg ccttcgactt cgtcgctggg ttgcatgcgg
     3841 ccaaagacaa cgcaaggacg acgacggctg cggctgcggg tacggctctg acctgcatgg
     3901 ctgctccctg ctcgtgcacg ggtagaccgg cggacgttcc gacgctaggt gcggaacgtc
     3961 cgcgcggaat cggggcaaac cctcgatcag ggcgggacgc cgatgtgctg gcgtacgctg
     4021 gccgcgcacc ggctccacct gccacgtcgc cacggggaga tccgaatgtt cacgtcggac
     4081 caaccgcgtg cgaactgacc cgccggatct catcgggcgc gcttctgagc gggctgcgat
     4141 cctgggtgcg cttcatgcat cagctaacgg accagccgcc gtcctgttga cgggcgacgc
     4201 aggcatcggc aagaccgcga tctgggagtc gatcgttgcg gagcaacgcg cggccggcga
     4261 tcacgtgttg gtctcgcggg caaccgcggc ggaggcccgc ttgccgtggg tcggcctcac
     4321 cgacttgatg cggacgatcc cgcccgaggc cctagcatcg ctgccggatg tccaacgcca
     4381 ggcgttagag gccgtgtcat tgcaggccgt gtcactgcaa cgcggtagct cggacgcgct
     4441 cgacgaacgc acagtcggca ccgcgctgtt gtcggcattg caagccgtcg ccaccaccgc
     4501 acccgtgctg ttggctattg acgacctgcc ctacttcgac acggccagtg catccgcagt
     4561 gacctttgcg ttacgaagaa tggaagggcg tcatcggacg cggctgctcg cgaccattcg
     4621 tgaccacgat ctgcggctgg tggcattgca gggtctgcca cgcgatcggt actccgttat
     4681 ctccgtcggt ccgttgacgc tgggggcact gttcgatctg ctgcagaccc gaagggggat
     4741 acggctcccc cgcccgctct tgctgcgcgt gtatgaaacg tcaggaggca acccgctcta
     4801 cgcgttggag ctggcgcggg cgttggaccg acttgagatc aatccaaagc ccggtattcc
     4861 gctgcctgta ccggacggac tcaacgccct ggtcgatgcg cgtgtgcgcg acctaccgac
     4921 ggaggtggcc gaggtcctcg ccgcgacagc ggcagcctgg cgagtcaacg ccgacgaaca
     4981 agatgccgag gcagtcgaac tcgccgtcgc ggcaggcatg gtcgtcatcg ccgagcccgt
     5041 ggtggttggt ggcgcgcgcg tgatccgatc gacccatccg ctcctgagtg cggcggcgta
     5101 caacggcctg agcacctccc ggcgtcgaaa gctgcacgcc cgacttgctg ccgctgccga
     5161 tgaccctgtc gagcgggtgc ggcatgccgc cctcgcggcg aacgaaccgc aagcggcact
     5221 cgctctggat ctagacgtcg gcgtcgccgc tgcgctcgcc gccggcgtcc cggatattgc
     5281 tgttggtctg gcgcagctgt cgctggaaca cacgaccgac atcgacctgc gtccggtgcg
     5341 cctcgaccgg ctcgccgacg cgcagctgcg ttccggagac agctcgggtg cttggcagac
     5401 ccaatcagag gcgattgcgg caaccgcaga aggggctgaa cgtgcgcgcc ggcgcatcag
     5461 attggccgag attgccgttg aggtgacgag ttgggcggac gccgaacgcg agctacaggt
     5521 cgccatcgcc gaggcaaccg acaaccccgt ggtgttggcc gaagcgttgc tgacgctcgc
     5581 agctgtcacc gacgacatcg acttggcgga gacctcggcg cagcgggccg tcgacatcct
     5641 tctcacgcag gatgatcccg acccgatgat tttgtcaggc gcactgagcc agcttgctgg
     5701 cgcgcgcttt cgggctggcc gtggactcga ccacgaactg ttcgcccggg cgatcgaaat
     5761 cgagcgtgcg catccctctc ggcgactctc cgatcgggcc gacgccagct atgcggcgct
     5821 gctgaagtac gccgatgacc tcgacggcgc agagggtctg ctgacgtccc tactaaagga
     5881 ggcacgggcg accggcgatc tctcgtccat cacctatgcg atcagccacc tcgtgcatct
     5941 ctacctgtgg cgaggtgagc tcgctcgggg gcgctcgtgt gccgacgaac acctcgaggt
     6001 cgcggtgcac ggcgagctcc agggtcaaga agaccaagcg cgctacaacg tcggccttgc
     6061 catggcgtgc cagggccagc tcgacgatgc cgatcgtctg ctactcgggc ttctcgagac
     6121 cgtgactgcg agcacccacg cctggctcgt tcatcgcgtc cacgcagtcc tcggctttat
     6181 tgcgctgtct cgcaacgatg cggtcaccgc ggctcagcac acagacttat ggcacgacgc
     6241 gctgatcgag atgcattttg gcgagcccgg ctatagccgt tcgcacctcg atcacctctg
     6301 cgcattggtc gacacaggcc ggacggcgga cgccgacgct ttctgcctcg aactcgacct
     6361 ccaggcaaag cgctcgggcc gcgcgtccgc ggccgccgtc gtgttgaccg gtcgcgccat
     6421 gctcgaagca catgcgggac gcctcagcga cgccgtggcc ttggttgcat ccgcactctc
     6481 ctggtacgac acctcgccgc tgcgatttga tcgtgcacga acgttgctga tcgccggccg
     6541 catcagtcga cgcgccaaag caaaatccga tgcacatgca ctgctcaccg aagccgagca
     6601 cgagttcgcg tccttcggcg caactgcgtg gcaggcgcaa gcggcggccg agctggcgcg
     6661 agtcaacgtg cggccggctg cgccggccga gctgacggag accgagcggt tggtcgcgca
     6721 gctcgcggcg tcgggactga ccaaccgcga ggtcgccgac cgcacattcc tcgcggtgaa
     6781 gacggtcgag gcaaacctcg cgcgtgtata tcgcaagctc ggcatccgtt cgcgcgccga
     6841 gctcggcgcc cggatgggag cgaacgcctc gtaacgccgg acgcctctag ccgccgttgc
     6901 gctcggcaaa catgagccga tcgcagcgaa ggtccgcaag ccgcgcaagg tcaagcactg
     6961 cagcttcgtc gactgccatg aactcgaaga acaccacctc gtcggtcggc atgagggtgc
     7021 tggcaacgaa cgtcgcgtcc tgggtgctca actgggtgag acggcgaccc accgcctcga
     7081 catcagcggc cgtgacactc ggccaatagc gctcgacgag aaatcccacc atgtgccgac
     7141 cgtaggtccg cgtcaggact cacgccagat ctccctccct gatcgagggt ttgcccggat
     7201 tcccccaccc ggccccgaat ctagcgtcga gaaggtcaag tacgacgccg aaccgaggag
     7261 ctcaaatgac cgcgtcgccg gccctcaccc gcctggcctt gctggccgcg accgcactcc
     7321 tgctcgccgg gtgcaacagt ggatcgtcgg ggaaagcctc gtcagctctg atcccgagtt
     7381 cggcgccggc agtcgactcc gcgagcgcag cgccaagcgc cggcgacgcg tcggatgcgc
     7441 cactcccggt cgccagcggt catccggcgg tgacctgcca gcagctgacg cttcagcaag
     7501 tgcagccgtt catcggcgag aagctgacgg gtgacgcggt gcagttcgag aacctgggcg
     7561 acgtcacagg tcaacagtgc acgttctctg gcgccagcga cgagcactcc atcaacatct
     7621 tggtgctgaa agggtcgggc gccgcagcgg cctacagttc ggaagtcgct gacgaggcag
     7681 acggccaggt cgacgtgcca gaagtcggcg acaaggcgag ccgcgataag ggcagcggtg
     7741 ggatcaatgc gctcaaaggc aacgtttatt gctcaaccgg cgtagacgac ggcctgccgg
     7801 cgatcggcac gctcgagagc gccgcaaacg gcacgacgaa catcggcgag gcctactacc
     7861 aagaggctgc gatcgccgtc gggacgctct gcaaccggat cttcggcagc ggcaacacca
     7921 cacctgactt gagcaagttg ctggcggccg ccgcggttgc ctcggcgtct gccgcggcca
     7981 cggcaagcgc gggggaagcc gcgcagtcgg cgttcgcttc tacctatgcc ccggccggct
     8041 agtcatgccc gtagctttcg gtcaggacag ctttgagatg atgatcttgg ccatggcgac
     8101 atcaggcgcg tagttgttct ggagttcggc gccggtcaga ccggacacct caaggcaccg
     8161 gctgccgacc ttcacgatga tgccgatcga gtatccgaac gcagcgtcac ccacgccaga
     8221 aaccggggtg ccggggccat ccgcctgctg caactgggtg tagcagtccg gtagcgagat
     8281 cacctggacg gtcaagtcct gaatgtcgac gggattcttt gaaccgttgt tcttgtaggt
     8341 gcacgagtca aagcccttct ccacctgctg tggcgtggtg gctccgtacg tcaccccgtt
     8401 gatcgacgat gcttgagctg acgtgaggag cccgcagacg tcgaccgtcc ctccacctga
     8461 cgcgggcggt ggcgtgacgg tcgcggcagc actggccggg ccggctgacg cactcgagcc
     8521 gaccgacggg ctggctgtcg agctcgtcgg tgacgtcggt gacgtcggtg ccgccgccgt
     8581 tgcggtcgcg ggcacccctg tccccacggc cgtggctttt gccttcgttg cggggttgca
     8641 cgcggtcgaa cacaacgcaa cgaccgcgac cgctgcggcc gccgaaagcg ctcggacctt
     8701 catagctgct ccctgctgaa aggatggtcc cggccaccgg tcgggtcgca gctacggaat
     8761 caatcgcccg taacgtccgc gtaattccag cagttcacgg tgctagatgc gtaggagccc
     8821 gtacggttca gagacaactc cgcacgcgcc ccaggttgga gctccaagga atgacagctc
     8881 cgagaaaggc cgggatcctg gttgccgccc tcgccttggt gggctccgcg agtttgggcg
     8941 tcttcatttg ggcggcttac aagaactacg agatc
//
LOCUS       DASWVF010000854         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_355958, whole genome shotgun sequence.
ACCESSION   DASWVF010000854 DASWVF010000000
VERSION     DASWVF010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355958"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1715)
                     /gene="mtrB"
                     /locus_tag="VGD55_15140"
     CDS             complement(12..1715)
                     /gene="mtrB"
                     /locus_tag="VGD55_15140"
                     /inference="COORDINATES: protein motif:HMM:NF040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEY0871733.1"
                     /translation="MLRRVTQRWRRSIQVRVVSSTLVVSAIVVALLGFVVLQQVKEGL
                     IQAKLRSSVAEESLGVTNARTLLSSETGTRDVQALLQDVVQTLSPVQGGRDGGYEVVG
                     LELVTGTKSRTAPALNNGLLASSVPPKLVDAVNVGNSQYYYTYTSLVYDTGQRTSSGA
                     HILASYPGIVIGSSMQVPQNSLNTYQFYYLFSLQQQQQTLALVWRTLLLSGIALVVLL
                     AGIAWLVTRQVVTPVRMAARIAERLADGRLQERMKPKGEDDLARLAASFNKMAESLQG
                     QIRRLEELSRIQRRFVSDVSHELRTPLTTVRMAADVLHEARSDFDPASARSAELLQTQ
                     LDRFESLLGDLLEISRHDAGAASLEADAIDVRDLVRRVVEATEPLAARRGSAVTLHLP
                     DEPCICEADGRRVERILRNLLVNAAEHGEGGPIDVHVVADEDVVAIAVRDHGIGLRPG
                     EASLVFNRFWRADPARARTTGGTGLGLSIASEDARLHGGRLQAWGERGKGSQFVLTLP
                     RQAGAAVGRSPIPVEPADAGPRQGGPGGQGEAGEAGNGVATTGRTHHPGSIVTLRGRR
                     E"
     gene            complement(1816..>2113)
                     /locus_tag="VGD55_15145"
     CDS             complement(1816..>2113)
                     /locus_tag="VGD55_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0871734.1"
                     /translation="IAIGDLSIDVAGHAVSRGGSQIPLTPLEFDLLVALAKKPRNVFS
                     RELLLEQVWGYRHAADTRLVNVHVQRLRSKVELDPERPEIVVTVRGVGYKAGPA"
BASE COUNT          362 a          716 c          677 g          358 t
ORIGIN      
        1 gccttcgctg cctactcacg gcgaccgcgc aacgtcacga tgctgccggg gtggtgagtg
       61 cggcccgtcg tcgcgacgcc gttacccgct tcgcccgcct cgccctgccc gccgggccca
      121 ccctgccttg gcccggcgtc ggctggctcg actggaatcg gtgagcggcc gaccgccgcg
      181 cccgcctggc gcggcaacgt cagaacgaac tgcgagccct taccgcgttc gccccacgcc
      241 tgcagccgcc cgccgtgcag ccgagcgtct tcggacgcga tcgacaatcc gagcccggta
      301 ccgccggtcg ttcgcgcgcg cgctggatca gcgcgccaga aacggttgaa cacgagtgac
      361 gcctcgccag gtcgcagccc aatgccgtgg tcgcgaaccg cgatcgccac cacgtcttcg
      421 tcggcgacca catgcacatc gatcggcccg ccctcgccgt gctctgccgc attgacgagc
      481 aagttgcgca gaatccgttc gacccggcgt ccgtctgctt cgcagataca gggttcgtct
      541 ggaaggtgca gcgtgacggc gctgcctcgg cgtgcggcga gcggttctgt cgcttctaca
      601 acgcgacgaa ccagatcgcg gacgtcgatc gcgtcggcct cgagcgatgc cgcaccggcg
      661 tcgtgtcggc tgatctcgag caggtcgccg agcagggatt cgaagcggtc gagttgggtt
      721 tgcaacagct cggcggatcg tgcgctcgcc gggtcgaagt cggaacgggc ttcgtgcaac
      781 acatcggccg ccatgcgcac agtcgtcagc ggcgtacgca actcatgcga cacgtcggag
      841 acgaatctgc gctggatgcg cgacagctct tcaagtcgcc gaatctgacc ttgcaaactc
      901 tcggccatct tgttgaacga ggcggcaagg cgagcaaggt cgtcttcgcc cttcggcttc
      961 atgcgctctt gcaagcggcc atcggcaagg cgctccgcga tgcgtgctgc catgcggacc
     1021 ggcgtcacga cctggcgtgt caccaaccac gcgatgccgg caagcaacac gacaagagca
     1081 atgccgctga gcagcagggt gcgccagacg agcgccaacg tctgttgctg ttgctgaagg
     1141 gagaacaggt agtagaactg gtaggtattg aggctgttct gcggcacctg catcgagctg
     1201 ccgatcacga tgccggggta tgaagcaaga atgtgggcac cactcgaggt gcgctggccg
     1261 gtgtcgtaca ccaggctcgt atacgtgtag tagtactgcg agttgccgac gttcactgca
     1321 tcgaccaact tcggcggcac gctgctcgcc agcagaccgt tgttcagtgc aggcgccgtg
     1381 cgcgatttcg tgccggttac gagttcgagg ccgacgacct cgtaaccgcc gtcgcgcccg
     1441 ccctgcactg ggctcagcgt ctgcacaacg tcctgcaaca acgcctgcac gtcacgggtg
     1501 ccggtctcac tcgagagcag cgttcgcgcg ttcgtgacgc cgagagactc ctcagcgacc
     1561 gacgatcgca gtttggcctg gatcaggccc tctttgacct gttgcaagac cacgaagccg
     1621 agcaaggcaa cgacgatcgc cgagacgaca agcgtgctgc tgaccacgcg tacctggatt
     1681 gagcggcgcc agcgctgggt gacccgccgc agcacgcgcc ttatggtccg gcgaacgcgg
     1741 ctggcgacga cgcgcagacg actgcttgcg gacagcgtca accgcctcga cgcgtcttgc
     1801 tcagaccctg tggcctcacg ctgggcctgc cttgtagccg acaccgcgca cggtcaccac
     1861 gatctcgggc cgttcgggat cgagttcgac cttcgaacgc aaccgctgca catgcacgtt
     1921 gaccaacctg gtatcggcgg cgtgccggta cccccacacc tgctcgagca agagctcgcg
     1981 ggagaacacg ttgcgtggct tcttggcgag ggcgaccagc aggtcgaact ccagcggtgt
     2041 caacggaatc tggctgccgc cgcggctcac tgcgtggccg gcgacgtcga tcgacaggtc
     2101 accgatcgcg atg
//
LOCUS       DASWVF010000855         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_381148, whole genome shotgun sequence.
ACCESSION   DASWVF010000855 DASWVF010000000
VERSION     DASWVF010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381148"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGD55_15150"
     CDS             <1..238
                     /locus_tag="VGD55_15150"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY0871735.1"
                     /translation="ESFHVDFGGELSPAATGEHPDYNWRNERAANDFAANLLMPAAVV
                     RTEFTKTADVNELADLFDVSPAAMGFRLTNLRLR"
     gene            complement(340..993)
                     /locus_tag="VGD55_15155"
     CDS             complement(340..993)
                     /locus_tag="VGD55_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0871736.1"
                     /translation="MGRRVRPSNFPTEIQQLADEVPGRYAALVHLLAWSGARIGELAI
                     LRVGSLDLDPQSPSVFITQRVYLINGVYDVDTPKSAAGRRRIALPPHLVPILLQHVET
                     FTTRKPDELVFTSTTGGSVLCTYSQMLSRALARIGRPDARVHDLRHTGMTLAAEAGAS
                     LADLKLRMGQSTTRAAELYIHATTDHGRSIANAMSALATSAGALAPRRDVRPRLGSP"
     gene            complement(1050..>1536)
                     /locus_tag="VGD55_15160"
     CDS             complement(1050..>1536)
                     /locus_tag="VGD55_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY0871737.1"
                     /translation="AAGADAEHLHERYIRNINAVLEHKPADMRVTTHMCRGNYRSSWT
                     ASGGYDFVAEALFGQLGVDGFFLEYDDPRSGDFSPLRFVPPGKMVVLGLVTTKRGALE
                     SKDDLKRRIDEASQYVPLEQICLSPQCGFSSTVEGNALTYDEEVAKLQLVVETAQDVW
                     G"
BASE COUNT          268 a          510 c          485 g          273 t
ORIGIN      
        1 cgaaagcttc cacgtcgact tcggaggcga gctttcacca gccgctaccg gcgaacatcc
       61 cgattacaac tggcgcaacg agcgcgccgc caacgacttc gccgccaact tattgatgcc
      121 cgctgccgtc gttcggacag agttcacgaa gaccgccgac gtcaacgaac tcgccgacct
      181 gttcgacgtc agtcccgcgg ccatgggatt ccggctaacg aaccttcgcc tccgctagat
      241 cactgccggt cgcggctccg ggcttcggtg atcaaagctc gcgcagatcg acgcttattt
      301 gcggagggac gcgccagcgg tctacttcgg tcgtcacggt catggacttc ccagccgagg
      361 ccgtacgtcc cgccggggcg cgagcgctcc agccgaggtg gccagcgccg acatagcgtt
      421 cgcgatgctg cggccgtggt ccgtcgttgc gtggatgtac agctccgccg cccgcgtcgt
      481 tgactgaccc atccgcaact tcaagtcggc caagctcgca ccggcttcgg ccgccagagt
      541 cataccggta tgccgaaggt catgcacccg cgcgtcaggc cggccgatgc gcgccagggc
      601 gcggctgagc atctgcgagt acgtgcagag caccgacccg cccgtcgttg acgtgaacac
      661 aagctcgtcc ggtttccgcg ttgtgaaggt ctcgacgtgc tggagaagga tcggcaccag
      721 gtggggtggc aaagcgatcc ggcgacgtcc ggcagcggac ttcggggtat cgacgtcgta
      781 gaccccgttg atcaggtaga cccgctgggt gatgaagacg ctcggcgact ggggatcaag
      841 gtcgagggaa ccgactcgca ggatcgccag ttcaccgatc cgcgcacccg accaggccag
      901 cagatgcacc aaggcggcgt accggccggg cacctcgtcc gcgagctgct ggatctccgt
      961 agggaaattc gaaggtctga cgcgccttcc caagctgaca gtgcgggttc gattcccgtc
     1021 acccgctcga cacgagccgg cgtatcgatt cagccccaga cgtcttgcgc ggtctcgacc
     1081 acgagctgca acttggcgac ctcttcgtcg tacgtgaggg cattgccctc gaccgtcgaa
     1141 gagaacccgc attgcggcga gagacagatc tgctccagcg gcacgtattg gctcgcctcg
     1201 tcgatgcggc gtttcaggtc gtccttgctc tccagcgcgc cgcgcttggt cgtgaccagt
     1261 ccgagaacga ccatctttcc tggcggtacg aaacgcagcg gtgaaaagtc gcccgaccgc
     1321 gggtcgtcgt attcgaggaa gaatccgtcg acgccgagtt gcccgaatag cgcttcggcg
     1381 acgaagtcgt agccgcccga cgcggtccac gacgaccggt agttgccccg gcacatatgg
     1441 gtcgtcacgc gcatgtccgc tggcttgtgt tcgagcaccg cgttgatgtt gcggatgtag
     1501 cgctcgtgca ggtgctcggc gtctgcaccc gcagcc
//
LOCUS       DASWVF010000856         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_253988, whole genome shotgun sequence.
ACCESSION   DASWVF010000856 DASWVF010000000
VERSION     DASWVF010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253988"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGD55_15165"
     CDS             <1..545
                     /locus_tag="VGD55_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0871738.1"
                     /translation="VANLRAFLAYMWAHPGKHLLFMGCEIAQDNEWSEERSLDWWLLE
                     YAEHNTVQRLVRDLNTTYRETPAMWATDGDPEAFRWIDANDASNNTFSFLRRAADGTT
                     LACIANFSGLPHENYRLGLPHPGRWDEVLNTDADIYGGSGVGNLGAVEATDEPWHGLP
                     ASTILRVPPLATVWLRSKPH"
     gene            649..>1548
                     /locus_tag="VGD55_15170"
     CDS             649..>1548
                     /locus_tag="VGD55_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY0871739.1"
                     /translation="MVVMRRLVAGQLWVRLLVALISVSLITGATLALRASQSRAKEGI
                     ETRFAARATLSATFVSTYVHQLTNREQLVATNTLSGADPSAAFASDVQAFGFQAGVLL
                     NSAGQALAIEPSAPQLIGQQYGTKYAHLAVALKGTVAVSNVVISAVKSAPVVAFAVPF
                     NTPSGRRVFSGAYIVNETPVAAFLSATTTLKHAVLYLTDGTGTVLASNGALTQEAQSL
                     SQRNRDLGSAAAQSQNGEYTSASVAYTFVKVAVPGTTWSLLISAPSSGVYVSVNGSTH
                     WLPWLILGGLSILMLVAAWLTIRL"
BASE COUNT          274 a          525 c          487 g          262 t
ORIGIN      
        1 aagtcgccaa cctgcgtgca ttcctcgcct acatgtgggc gcaccccggc aagcacctgc
       61 tgttcatggg ctgcgagatc gcccaagaca acgagtggtc tgaagagcgc tcgctcgact
      121 ggtggctgct cgagtacgcc gaacacaaca cggtgcagcg actcgtgcgc gacctcaaca
      181 cgacctatcg cgagacgccg gccatgtggg cgactgacgg cgatcccgag gcgttccgct
      241 ggatcgacgc gaacgacgcg agcaacaaca cgttctcgtt ccttcgccgg gccgccgacg
      301 gaacgacgct ggcctgcatc gcaaacttct cagggctccc gcacgagaac taccgcctcg
      361 ggctgcccca cccaggccgc tgggacgagg tgctgaatac cgacgccgac atctacggag
      421 gtagtggggt cggcaacctc ggggcggtcg aggcgaccga cgagccgtgg cacggcctgc
      481 cagcctcgac gatcttgcgg gtgcctccgc tggcgaccgt ctggctgcgc agcaagcctc
      541 actaagcgcc cgcagctgag caaccgggcc tggcgagggt gaatatgaga ggaaagtgtc
      601 agatcgctcc ctgaggtgcc gagagggctc ccgaggagtt gaggcgctgt ggtcgtcatg
      661 cgccggctgg ttgccgggca gttgtgggta cggctgctgg ttgcgttgat ctcggtctcg
      721 ctcatcaccg gagccacgtt ggcgctgcgc gcctctcaat cgcgcgccaa ggagggcatc
      781 gagactcgct ttgccgctcg ggcgaccctc tcagcgacct tcgtaagcac ctacgtacac
      841 cagctgacca accgcgagca gctagtggca acgaacaccc tgagcggcgc agacccgtcc
      901 gcggcattcg cgtcggacgt ccaagccttt ggtttccagg ccggcgtcct gctgaactcc
      961 gccggccaag cactggcaat cgagcccagc gcaccccagc tcatcgggca gcagtacggc
     1021 acgaagtacg cccacctcgc ggtcgccctg aagggcaccg tcgcggtctc gaatgtcgtc
     1081 atttctgccg tcaagagcgc ccccgtggtc gcgttcgccg tcccgttcaa cacgccgagc
     1141 ggacggcgcg tgttcagcgg cgcctacatc gtcaacgaga cgccggtcgc tgcgttcctc
     1201 agtgccacga ccaccttgaa gcacgcggtg ctctacctca cggacggcac cggaacggtg
     1261 cttgcttcga acggtgcact gacgcaggag gcgcaaagcc tgtcgcaacg aaaccgagac
     1321 ctcggaagtg ccgcagcgca aagccagaac ggagagtaca cgtcggcttc cgtcgcttac
     1381 accttcgtca aggtagccgt gccgggtacg acctggtcat tgttgatctc ggcccctagc
     1441 agcggcgtct acgtctccgt caatggcagc actcactggt tgccgtggtt gatcctcggc
     1501 ggcctgtcga tcctcatgct ggtggcagcc tggctgacga tccgcctg
//
LOCUS       DASWVF010000857         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_177074, whole genome shotgun sequence.
ACCESSION   DASWVF010000857 DASWVF010000000
VERSION     DASWVF010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177074"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /gene="alaS"
                     /locus_tag="VGD55_15175"
     CDS             complement(<1..1741)
                     /gene="alaS"
                     /locus_tag="VGD55_15175"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0871740.1"
                     /translation="MTVYTDDDEAAAIWHDVVGVPAQRIQRRGMADNFWSMGVPGPCG
                     PCSEIYYDRGPAFGVGGGPIANEERYLEVWNLVFMQFERGAGGTKESFPILGELPAKN
                     IDTGMGLERMAAILQGVDNLYEIDSTRPILDRAAELSNQRYGANADHDVRLRVVADHS
                     RAATMLIGDGVTPSNEEAGYVLRRILRRIVRNMRLLGAHGPTMHELIATTIHVMSPQY
                     PELARDAARIHAVADGEETSFFSTLQAGTTRFENAARESRGAVLSGEQAFALHDTYGF
                     PIDLTLEMAAEQGLSVDEAGFRRLMEEQRQRAKADSKKKKTGAVDVSAYRAALDAVGA
                     PVEFTGYRELSGDAHVRGVLVDGALVDAASAGQQVELILDRTPFYAEGGGQLADAGVI
                     RLANGTVIEVDDVQQPLPGLIVHRGRVISGEAVVGEEAHAAIDVERRRAISRAHTATH
                     MVHKAFREALGDSATQAGSENSPGRFRFDFANPSAVPASVLADVEARVNDVLVDDLLV
                     TADVMSQAEAVASGAMALFGEKYGDRVRVVSVGDWSRELCGGTHVASTGHLGLVKLLG
                     ESSLGAGTRRVEAL"
BASE COUNT          304 a          631 c          547 g          292 t
ORIGIN      
        1 cgagcgcctc gacccgccgg gtaccggcac cgagcgagga ttcaccgagc aacttgacca
       61 gccccagatg cccggtgctg gcgacgtggg tgccaccgca cagctcgcgg gaccagtcac
      121 cgacggacac gacgcgcacc cggtcgccgt acttctcgcc gaacagcgcc atcgcgcccg
      181 aagcgacggc ttcggcctgg ctcatcacgt ctgccgtcac gagcaggtcg tcaacgagca
      241 cgtcgttcac ccgtgcctcg acatcggcaa gcaccgacgc gggcaccgcc gacgggttgg
      301 cgaagtcgaa cctgaaccgg ccgggcgagt tctccgaccc ggcctgggtt gcggagtcgc
      361 ccaacgcctc gcgaaaagcc ttgtgcacca tgtgggtcgc ggtgtgcgcc cgcgagatcg
      421 cccggcggcg ttcgacgtca attgcggcat gcgcctcttc gccgacgacc gcctcgcccg
      481 agatgacgcg gccccgatgc acgatcaacc ccggcaacgg ctgctgcacg tcatcgacct
      541 cgatcacggt gccgttcgcc agcctgatca cgccggcgtc ggcgagctgc ccaccgccct
      601 cagcgtagaa cggcgtgcga tcaaggatga gctcgacttg ttggcccgcc gatgccgcgt
      661 cgaccaacgc accgtcgaca aggacaccgc gaacgtgtgc atcgccagaa agctcgcgat
      721 agccggtgaa ctcaaccggc gcaccgacgg cgtcgagcgc cgcacgatac gccgagacgt
      781 caaccgcgcc cgtcttcttc ttcttcgagt cagccttcgc gcgctgccgc tgctcttcca
      841 tgagccgacg aaaacctgct tcgtcgaccg aaaggccttg ctctgcagcc atttccaacg
      901 tcaagtcaat cgggaagccg tacgtatcgt gcagcgcgaa ggcctgctcg cccgacagca
      961 ccgcaccgcg cgactctctc gccgcgttct cgaaacgcgt ggtgccggct tgcagcgtcg
     1021 agaagaacga ggtctcctcg ccatcggcta ccgcgtgaat gcgagcagcg tcgcgggcga
     1081 gttcggggta ctgcggcgac atcacgtgga tcgtcgtcgc gatcagctcg tgcatcgtgg
     1141 ggccgtgtgc accgagtagc cgcatgttgc gcacgatgcg ccgcaggatc cggcgcagca
     1201 cgtagcccgc ttcttcgttc gacggcgtga cgccgtcgcc gatcagcatc gtcgctgcac
     1261 gcgaatgatc ggcgacgacg cgaagccgta catcgtggtc ggcgttcgcg ccgtaccgct
     1321 gattgctgag ttctgctgcg cgatcgagga tcggcctggt gctgtcgatc tcgtagaggt
     1381 tgtcgacgcc ttgcaggatc gcggccatcc gctcgaggcc catgccggtg tcgatgttct
     1441 tcgccggcaa ttcaccaagg atcggaaagc tctccttggt gccaccggcg cctcgctcga
     1501 actgcatgaa cacgaggttc cagacctcga gatagcgctc ctcgttcgcg atcggaccgc
     1561 cgccgacgcc aaaggctggc ccgcggtcgt aatagatctc gctgcacgga ccacacgggc
     1621 ccggcactcc catcgaccag aagttgtcgg ccatgccgcg ccgttgaatg cgttgcgccg
     1681 ggacgccgac cacgtcgtgc cagatcgcgg ctgcctcgtc gtcgtcggtg tagaccgtca
     1741 cccacagcag ctcaggatca aagccatacc cgcc
//
LOCUS       DASWVF010000858         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_152014, whole genome shotgun sequence.
ACCESSION   DASWVF010000858 DASWVF010000000
VERSION     DASWVF010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152014"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VGD55_15180"
     CDS             complement(<1..1264)
                     /locus_tag="VGD55_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871741.1"
                     /translation="MATVMSDPFSGTVGNTFAGDAVYSGLLSTAPKFVADTKYGVGLQ
                     FTAATNGTMDETFSATSQRVFDRVLLISATPSAACDFFEARAAGANTCKAGVGATGKL
                     QLKNAAGTSLTGGTSANVLPVGTRFRLVINMNGTSLTAVIYNNDTDTTVLENLNPSGT
                     IVSASPTIAREGLITAGGMGAGVAFTMFWPMDGDTVETGLRPVWGPWQSPPRRRVQSA
                     RPRPAVQLVPQPLAVPATVSARSRRPAALAPRRPRPSTLPLVAQVTAPVVYVPPAVRR
                     RLAQLVTRRRPAAPLPSFLPASLASAPRSSRRPQLLFGRRPRSGFVPPPVVTVTPAGF
                     VPGSPRPRRLLGIRRRVAGSPPFSVAATSTPTLFLPSSGTRRLRPSPRRSTATWMPPT
                     APVSQLSLLLVAPRRLARLLVWRRPVTRL"
     gene            complement(1318..1833)
                     /locus_tag="VGD55_15185"
     CDS             complement(1318..1833)
                     /locus_tag="VGD55_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871742.1"
                     /translation="MARYGTSFVSQTAVLAGVNATTTVNGYLGALIGGASANPKLRRM
                     MIGCRTASAVPIVSQQFTIGIYPQTVRAVGTGLSNVAGNKLDPNSPADPTGGVDFTTA
                     TTLGTSGPTLAANPTASPTFNTQANGDSPWENLEELYTGLGTANGLAFVNIGNTFQAG
                     TFIVVSLEWEV"
     gene            complement(1836..2126)
                     /locus_tag="VGD55_15190"
     CDS             complement(1836..2126)
                     /locus_tag="VGD55_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871743.1"
                     /translation="MTTSLVAALHADLDDVEARIKAAYQPEIDRLREYGRDEERAIEK
                     RQVATAHANGIASKLESITAHLPTLHMLIDNLHRDHPDADPATAAAATSTEA"
     gene            complement(2164..>2521)
                     /locus_tag="VGD55_15195"
     CDS             complement(2164..>2521)
                     /locus_tag="VGD55_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871744.1"
                     /translation="SDGVQILSGSNLKVIGNTIVGQPNSAIIITQDHGAVSGVLIDGN
                     WLGSQGGTQIATIKILNKPLPSLSGITVTNNRFEAPLASACQILDSKTVTLTQFGNVI
                     DSSGVPAKLLNTASVT"
BASE COUNT          420 a          807 c          869 g          425 t
ORIGIN      
        1 ccagacgtgt aaccggtcga cgccacacga gcaatcgtgc aagtcgccgc ggtgcgacca
       61 gtaacagcga aagctgcgac accggcgccg tcggtggcat ccaggtcgcc gtactgcgcc
      121 gtggtgatgg ccgcaaacga cgcgtgccgc tcgagggaag gaacagtgtt ggtgtcgacg
      181 tcgcggcgac cgagaacggc ggcgaccctg caactcgtcg acggatcccc agcagccggc
      241 gcggccgcgg cgaacccggc acgaagcctg ccggcgtcac cgtcacgacc ggcggcggga
      301 cgaagccgct gcgcggccga cgaccgaaca gcagctgcgg ccgcctcgat gacctcggcg
      361 ccgatgcaag gctcgccggc agaaacgacg gcaacggcgc ggccggcctg cggcgagtga
      421 ccagctgcgc cagcctgcgg cgaaccgctg gcggcacgta cacaaccggt gcggttacct
      481 gcgcgacgag cggcagcgtg gacggccgcg ggcgacgcgg tgcgagtgca gctggtcgac
      541 gtgatcgggc agacaccgtc gccggaacag cgagcggttg aggcacgagc tgcaccgccg
      601 gccgcggccg cgctgactgc acacgacgtc gcggtggcga ctgccacggt ccccacactg
      661 gccgcagccc ggtctcgacg gtgtcgccgt ccatcggcca gaacatcgtg aacgccacac
      721 cggcgcccat cccgccagcg gtgatcaggc cctcgcgggc gatcgtcggc gacgccgaga
      781 cgatcgtgcc ggacgggttc aggttctcga gcaccgtggt atcggtgtcg ttgttgtaga
      841 tcactgcggt gagcgacgtg ccgttcatgt tgatcacgag acggaaccgg gtgccaaccg
      901 gcaacacgtt cgccgaggtg ccaccggtca ggctggtacc cgcagcattc ttcagctgca
      961 gcttcccggt cgcaccgaca ccggccttgc aggtgttcgc acccgctgcg cgggcctcga
     1021 agaagtcgca cgctgctgac ggtgtcgccg agatcagcag gacccggtcg aacacgcgct
     1081 gcgacgtcgc tgagaacgtc tcgtccatcg tgccgttggt ggctgcagtg aactgcaacc
     1141 cgacgccgta cttcgtgtcg gcgacgaact tcggcgccgt cgacagtagg ccgctgtaca
     1201 cggcgtcgcc ggcgaaggtg ttgccaaccg tgccgctgaa cggatcgctc atgacggtcg
     1261 ccatgtcacg tcctcaccgt tcagcggcca ccgactatcg gatcgaacgc cgagtgctca
     1321 gacttcccat tcgagcgaca cgacgatgaa cgtgcccgct tggaaggtgt tgccgatgtt
     1381 gacgaacgcc aagccgttcg cggtgcccag accggtgtaa agctcctcga ggttctccca
     1441 cggggagtcg ccgttggcct gggtgttgaa cgtcggcgac gctgtcgggt ttgccgccaa
     1501 cgtcggaccc gaggtgccga gcgtcgtcgc cgtagtgaag tccacaccgc cggtcggatc
     1561 agcgggcgag ttcgggtcga gcttgttgcc ggcaacgttc gacaaaccgg ttccgaccgc
     1621 acgaacggtc tgcgggtaga tgccgatggt gaactgctgc gacacgatcg gaacggctga
     1681 cgcggtgcga caaccgatca tcatgcgccg cagcttcggg ttcgccgaag cgccaccaat
     1741 caacgcgccg aggtagccgt tgacggtcgt ggtcgcgttg acgccggcca ggaccgcggt
     1801 ctgtgaaacg aacgaggtgc cgtagcgggc cattgtcatg cctccgtcga agtagcagca
     1861 gctgcggtcg ccgggtcggc gtccggatgg tctcggtgca ggttgtcgat gagcatgtgc
     1921 aacgtcggaa ggtgcgcggt gatcgactcc aacttcgacg cgatgccgtt cgcgtgcgcg
     1981 gtcgcgacct ggcgcttttc gatcgcgcgc tcttcgtcgc gtccgtactc gcgaaggcgg
     2041 tcgatctccg gctggtaggc agccttgatc cgtgcctcga cgtcgtcgag gtcggcgtgc
     2101 aatgcggcga cgagggacgt ggtcatttgc tgctccttct gacgtgcgga catggttggc
     2161 gtgctacgtg accgaggcgg tattcagcag cttcgccggc acgccggagc tgtcgattac
     2221 gttgccgaac tgcgtcagcg tcaccgtttt ggaatcgagg atctggcacg cggacgccaa
     2281 gggtgcttcg aaacggttgt tggtgaccgt gatccccgag agcgacggca gcggcttgtt
     2341 gaggatcttg atggtggcga tctgcgtgcc gccctgactg cctagccagt tgccgtcgat
     2401 gagcacaccg gacaccgcgc cgtggtcttg ggtgatgatg atcgccgagt tcggctgacc
     2461 gacgatggtg ttgccgatca ccttcagatt cgagcccgac aggatctgca cgccgtctga
     2521 g
//
LOCUS       DASWVF010000859         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_203185, whole genome shotgun sequence.
ACCESSION   DASWVF010000859 DASWVF010000000
VERSION     DASWVF010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203185"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="dusB"
                     /locus_tag="VGD55_15200"
     CDS             complement(<1..944)
                     /gene="dusB"
                     /locus_tag="VGD55_15200"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0871745.1"
                     /translation="MALKIGPIEVDPPIVLAPMAGVTNLPFRRLCRGYGAALYVSEMV
                     SARAVVERNPNTDAMLRFGADEPVRSLQLYGVDASVMYDAVRYAVDEVGVDHIDLNFG
                     CPAQKVNRVGGGAALPLRRRLFADIVQAAVRAGGAVPVTVKMRKGVDDATLTYLESAR
                     AAEDAGVAAIALHARTAEQLYSGAADWSAIACLREEITSIPVLGNGDIWAATDALAMM
                     RETGCAGVVVGRGCLGKPWLFRELTQAFADEPIDDAPLLGEVVAVMRHHVGLLIAHKR
                     TPQLGVRAFRKHLGWYLTGFPVCAHIRRALMDAETPAEL"
     gene            complement(935..>1612)
                     /locus_tag="VGD55_15205"
     CDS             complement(935..>1612)
                     /locus_tag="VGD55_15205"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY0871746.1"
                     /translation="GADRDLRYARDPDACRPMSPPTAMMTSMASRQLVLASASPARLR
                     LLWDAGFDPVVLVSGVDESAVESDDHVELVVTLARRKANSVASIAPSDSVVIGCDSML
                     SLGGDALGKPSNPDVAREYWERMAGREGVLLTGHCVIDTITGRESSAVARTTVHFGTP
                     TKAELDSYLATGEPLRVAGAFTLDGLAAPFVDGIEGDHGNVIGLSLPLLRRLLADLDI
                     GITDLWR"
BASE COUNT          297 a          533 c          519 g          263 t
ORIGIN      
        1 agttcggcag gagtttcggc gtccatcaaa gctcgtcgta tgtgggcgca gacggggaat
       61 ccggtgaggt accaaccgag gtgtttgcgg aaggcgcgca ctccgagctg cggcgtgcgc
      121 ttgtgggcga tgagcaggcc gacgtgatgg cgcatgacgg cgacgacctc accgagcagt
      181 ggtgcgtcgt cgatcggctc atcagcaaat gcttgggtaa gttcacggaa caaccacggc
      241 ttgccgagac agccgcgacc gaccacgacg cctgcacagc cggtctcgcg catcatcgcg
      301 agtgcgtcag tggcggccca gatgtcaccg ttgccgagca ctgggatcga ggtgatttct
      361 tcgcgcaggc aagcgatcgc cgaccaatcg gcggcacccg aatagagctg ctccgcggtt
      421 cgcgcgtgca acgcgatcgc tgcgacgcca gcatcttcgg ccgctctcgc ggactcgagg
      481 tacgtcagcg tcgcgtcgtc gactcccttg cgcatcttga cagtgacggg caccgcgcca
      541 cccgcccgga ctgcggcttg cacgatgtcg gcgaacagtc gtcggcgcaa cggcaacgca
      601 gccccaccgc cgacgcgatt gaccttctgt gccggacatc cgaagttgag atcgatgtgg
      661 tcgacgccga cctcgtcgac cgcgtaccgc acggcgtcgt acatcacgct cgcgtcgacg
      721 ccatacagct gaaggctgcg caccggctcg tcggcaccga accgcagcat cgcgtcggtg
      781 ttcgggttgc gctcaaccac cgcgcgcgcc gacaccatct cgctgacata cagcgccgcg
      841 ccgtaacccc ggcagagtct gcggaacggc aggttcgtga cgcccgccat cggcgcgagc
      901 acgataggtg ggtcgacttc gatcggccct attttcagcg ccataggtcg gtgatcccga
      961 tatcgaggtc ggcaagcagc cggcgcagca acggcaggga gaggccgatc acgttgccat
     1021 ggtcgccttc gatgccatca acgaacggcg ccgccaaccc gtcaagagtg aacgcgccgg
     1081 cgactcgcaa tggttcgcca gtagctaggt aggaatcgag ctcggctttc gtcggcgtgc
     1141 cgaaatgcac agtcgttcgc gcgacggccg acgattcgcg gccagtgatg gtgtcgatca
     1201 cgcaatgacc ggtgagcagc acaccctcac gcccggccat gcgttcccag tactcacgcg
     1261 cgacgtcggg gttacttggc ttgcccagcg catcgccgcc gaggctcagc atcgaatcac
     1321 acccaataac cacagagtcg gacggcgcaa tgctggcgac cgaattcgct ttgcgacgcg
     1381 cgagcgtcac aacgagctcc acgtggtcgt cgctctcgac agcgctctcg tcgacgccgc
     1441 tgacgagcac caccggatca aacccggcgt cccatagaag tcgcagtctc gcgggggatg
     1501 ccgacgcaag gacgagctgt cgtgacgcca tgctggtcat catcgcagtc gggggggaca
     1561 tcgggcggca tgcgtcaggg tcgcgggcgt acctaaggtc gcgatcggct cc
//
LOCUS       DASWVF010000860         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_152061, whole genome shotgun sequence.
ACCESSION   DASWVF010000860 DASWVF010000000
VERSION     DASWVF010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152061"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGD55_15210"
     CDS             <1..927
                     /locus_tag="VGD55_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0871747.1"
                     /translation="PPLVIPELSTARLSLRPLDPIRDATDLFEMDRDPRVHEYVYGSS
                     SSQSVEETRQRLDAELAGNGGVTWTIRLHDDPTALGTLGLFGDQGTSIRGVSWSLRPS
                     HWRQGITSEAAMAMVIYLLAQPGVEGLEAWADSRNIGSLRVATRAGMAERARLPRSYD
                     DRIGQTVVMAVAADPTDPRVLTVTPSLQVRDVDGTASLLCDLLSLRLAWSYGQPTAAA
                     GLAISRWSGSPEVHLHAAAGDVPVGELGVEVGGWIDDVHDAAVAAGLAILDAPADRDW
                     GRREFTFALREGHRISVSGATTPTGLAALRNV"
     gene            1004..1492
                     /locus_tag="VGD55_15215"
     CDS             1004..1492
                     /locus_tag="VGD55_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0871748.1"
                     /translation="MSVIDELLDNNRRYAETFVGMVPIRPAKKLAVVACMDSRIDVHA
                     LLGLHVGDAHIIRNGGGVISPDVIRSLTISQRVTGTREIILIHHTDCANLGLDEMAFK
                     AELQAEVGFKPDWSFDSFTDLEEDLRQSVLRLRSNPFIAYTDKIRGFIFDVATGKLAE
                     VV"
     gene            complement(1525..2151)
                     /locus_tag="VGD55_15220"
     CDS             complement(1525..2151)
                     /locus_tag="VGD55_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871749.1"
                     /translation="MSTKSRLVTVTNLALVASAACLLTACNKSSSASTPGASGATAAA
                     GAVSAPAGAAAAIAAATGNGGPLDSSKQCAAIKPADVQALMKAPVTAVQNFPGECSFY
                     GGDLKIDIYPNDPTQKYYQNPVNGPGTTPLSGVGDQAQWFAPVVGGTTPWVEAHKGSL
                     TCVVSPADVAETTITYTGSDPFFKIAPADSATYAAKEGALCSDIFGAS"
     gene            2259..5246
                     /locus_tag="VGD55_15225"
     CDS             2259..5246
                     /locus_tag="VGD55_15225"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0871750.1"
                     /translation="MRIDVLGQVAVTDGHAQVAGQALGGRRARVALVALALSGQTVPA
                     DRLASLIWAGDLPTTWQVALRGIVRGLRGVLAPIGGGDQQVISTAPSGYRLASGVEVD
                     VRLAEQAVRRAAELLAEGRPAAALELAQPIAEQSGEQLLPGEDAAWLAPHRIATDSVA
                     ARALELVVDAASRLGDHHTAIAAARRAVAATPLDERAHRSLIRALDESGDRAGAVRAY
                     EQCRAELADQLGIDPAAETVEVYLAALRDQTASSSARLPVISSTFVARDAEVAQLEAA
                     LAEPGLITVTGKGGVGKSRLAVRVAATRRDFVGGRLWVSLAPVAQDALVASTVALEVG
                     VPLGTDDATAALADHLAPLGRVLLVLDGCEVVIDGVASLANAVLASCPLLTLVVTSRV
                     PLSLEGERVVSIDPLDAPTADDAAALRANAQVRLLIDRVHDSGGDLQVDERLAPHLAL
                     LLRQCGGLPLALELVAAQLSAMPVGDLLDHLSDVLVDGEDRLRAVVRGSYALLDDDEA
                     AVYRRFAVLDGPVGLPLIRQVVSGGPIVPVRVVRILRELTARGLLAVDRSGARWRYHQ
                     DDDLHRFARELLVDHGEESAAFDRLADALRAALPADARETPASYREAVTDMLGSVRSL
                     FGAAISGHADRDRCLELAFRLHRYWAATNVAEGRYWLSRLLAEGGRSEWTPYATYAMG
                     YLDYWSGDTASAIHELETVVRLLGGVEDPYTAHALIYLAGLLDDLDRGAEALEYVRQA
                     IVAARPFSTDLQVTAAMGMGSVLSERGEADAARYAADAIALCRAGGSPEQLAAAMPTA
                     AMVCWQVGALDEARAYVAEARPMHSDSRRIARVVLLSTSAGLALAEGDIDAAVDFGEA
                     ADREATELGVEREVPLIRSVLARALLARGDVVGAAERAIAAFDAAFSITFEFPLAICL
                     ETAALVLRAARVSGGELAELLASAEVIRQRGDRPPPAPLAADIESLRADVGAELLAVR
                     LIGAREAGTLAVAKLSALPPRHE"
     gene            5492..5743
                     /locus_tag="VGD55_15230"
     CDS             5492..5743
                     /locus_tag="VGD55_15230"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY0871751.1"
                     /translation="MPLYMDTHHKVDGLTAEAVAGAHARDLEVQRAHDVNYLQYWFNE
                     DTGTVFCLVEAPNPEAAHAVHREAHGLVADEITEVRPGA"
     gene            5753..>6024
                     /locus_tag="VGD55_15235"
     CDS             5753..>6024
                     /locus_tag="VGD55_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871752.1"
                     /translation="MWNTLRVTEQPSSGAFVGRVAELDRLDAALAALAERGAAAVLVG
                     GDAGMGKSRLIEEFCARARSAGTLVAVGACTPAEGGGLPFGPVVGV"
BASE COUNT          900 a         2036 c         2074 g         1014 t
ORIGIN      
        1 ccgccgctcg tcatcccgga attatccacc gcgcgcctca gcctgcggcc actcgacccg
       61 attcgcgacg ccaccgacct cttcgagatg gatcgcgacc cgcgcgtcca tgaatacgtg
      121 tacgggtcgt cgtccagcca atcggttgag gaaacgaggc agcgcctcga cgccgagctt
      181 gccggcaacg gtggcgtgac ctggacgatc cggctgcacg acgacccgac agcgctcggg
      241 actctcgggc ttttcggtga tcaggggacg tcgattcgcg gcgtcagctg gagtctgcgc
      301 cctagccatt ggcgacaggg cattacgagc gaggccgcga tggcgatggt catctatctg
      361 cttgcgcagc cgggcgtcga aggactagag gcgtgggccg actcgcgcaa catcggctcg
      421 ctgcgcgttg ccacgcgggc gggcatggcg gagcgggcgc ggctgccgcg ctcgtacgac
      481 gaccgcatcg gccagacggt cgtcatggcg gttgccgctg atccgaccga tccccgcgta
      541 ttgaccgtga cgccttcact acaggtgcgg gacgtcgacg gtacggcgtc gctgctgtgc
      601 gatctgctgt cgttgcgcct ggcgtggagt tacgggcagc cgaccgccgc cgccggactc
      661 gcgatctcgc gatggtcggg aagtcccgag gtgcatctgc acgccgccgc cggcgacgtc
      721 ccggtcggtg aattgggcgt cgaggtcgga gggtggatcg atgacgtcca cgacgccgcc
      781 gtggccgcag gcctcgcgat cctcgacgcc cccgccgatc gcgactgggg tcgacgggaa
      841 ttcacgttcg ccctacgcga agggcaccgc atcagcgtgt cgggtgcgac gaccccgacc
      901 ggcttggctg cattgaggaa cgtgtaagtc ccaggacgct ctagagagcg tcctaggact
      961 tacaactatc gggttcggtc gggagtcgtg aaacgatgac cccatgtccg tgatcgatga
     1021 gctcctcgac aacaaccgcc gctacgccga gaccttcgtc ggcatggtcc cgatccgccc
     1081 agcgaagaaa ctcgcggtcg tcgcgtgcat ggactcgcgc atcgacgtcc acgcgcttct
     1141 tggcttgcac gtcggcgacg cgcacatcat ccgcaacggc gggggggtga tcagccccga
     1201 cgtaatccgc tcgctgacga tcagccagcg agtgaccggc acccgcgaga tcattctgat
     1261 ccaccacacc gactgcgcaa atctcggact cgacgagatg gcgttcaagg ccgaactcca
     1321 agccgaggtg ggatttaagc ccgactggtc gttcgactcg ttcaccgatc tcgaagagga
     1381 tctgcgccag tcggtgttgc ggctgcggtc gaaccccttc attgcctaca cggacaagat
     1441 tcgcggcttc atcttcgacg tcgccacggg caagctcgcc gaggtcgtct agagcgccga
     1501 ccaccttcga atggcggacg gcgcctagct cgcaccgaag atgtcgctgc agagtgcgcc
     1561 ttccttggcg gcgtacgtag cggagtcggc tggggcgatc ttgaagaacg ggtcgctgcc
     1621 cgtgtaggta atcgtggtct ccgccacgtc ggctggtgac accacgcagg tgagcgaacc
     1681 cttgtgtgcc tcgacccatg gcgtggtccc cccaacaacc ggcgcaaacc actgcgcctg
     1741 gtcaccgacg ccactaagcg gcgtcgtgcc cgggccgttg accgggttct ggtagtactt
     1801 ctgcgtcggg tcgttcgggt agatgtcgat cttgagatct cccccataga acgagcactc
     1861 gcccgggaag ttctggaccg cggtcacggg tgccttcatc aacgcctgca cgtcggcggg
     1921 cttgatcgcc gcgcactgct tggaactgtc cagcgggcca ccgtttccgg tcgcggctgc
     1981 aatggccgcc gcggcgccag cgggcgcact gaccgcaccg gcagcggcag tcgcgcccga
     2041 agcgcccggc gtcgacgccg acgaactctt gttgcacgcc gtcagaaggc atgccgcgct
     2101 cgcgacgagg gccaggttcg tgacggtgac gaggcgtgat ttcgttgaca tctgaacgct
     2161 cctagtgggc tcgcgattcg gttcctcgca accagttcag cgacccccag taacggctcg
     2221 gtaattccac gagtgcgggt ctgacctaag gttcccccgt gcggatcgat gtcctcggcc
     2281 aggtcgccgt gacggacggc cacgcccagg tggctggcca ggcattgggt ggccgccgtg
     2341 cacgtgtcgc gctggtcgcg ctggcgctat ccggccagac ggtgccggca gaccggctcg
     2401 cgtcgctgat ctgggccggc gacctcccga ccacgtggca ggttgcgttg cgcggcatcg
     2461 tgcggggcct tcgcggtgtc cttgccccga tcggtggcgg cgatcagcag gtcatcagca
     2521 ccgcgccgtc cggctatcgg cttgctagcg gggtcgaggt cgacgtccgg ctcgcggaac
     2581 aggccgtccg tcgcgcggcc gaacttctcg cggaaggacg acccgcggcc gcgctcgaac
     2641 tggctcagcc catcgccgag caatccggcg aacaactgct tccgggcgag gacgccgcgt
     2701 ggctggcgcc gcaccgcatc gcgaccgact cggtggcggc gcgagcgctc gaactcgtcg
     2761 tcgacgcagc gagccggctc ggcgaccacc acacggcgat cgccgcggca cgccgcgccg
     2821 tagcagcaac gccgctcgac gagcgcgcgc accggtcgct gatccgcgcg ctcgacgagt
     2881 cgggtgatcg cgccggcgca gtgcgggcct acgagcaatg tcgcgccgaa ctcgctgacc
     2941 agctcggcat cgaccctgcg gcggagaccg tcgaggtcta cctcgccgca ctacgcgatc
     3001 agacagcgtc gtcgtcagcc cggttgccgg tcatcagctc gacgttcgtc gcccgcgacg
     3061 ccgaggtcgc gcagctcgag gccgccctcg ccgagcccgg cctgatcacc gtcacgggca
     3121 agggcggggt cgggaaatcc cggctcgcgg tgcgggtggc ggcgacccgg cgtgacttcg
     3181 tcggtggccg gctgtgggtg tcgctcgcgc cggtcgcgca ggacgcactc gttgcatcga
     3241 ctgtggcgtt ggaggtcggc gttccgctcg gcaccgatga tgcgaccgcc gcactcgctg
     3301 accacctcgc gccacttggc cgggtgctgc tcgtactcga cgggtgcgag gtcgtcatcg
     3361 acggggtcgc atcgcttgcc aacgcggtgc tggcgagctg cccgctcctc acgctggtgg
     3421 tgacgagtcg ggttccgctg tcactcgagg gtgagcgtgt cgtcagcatc gacccacttg
     3481 acgcgccgac ggccgacgat gccgccgctt tgcgagccaa cgcgcaagtt cgccttctga
     3541 tcgaccgggt gcacgacagc ggcggtgact tgcaggtcga cgagcggctc gcgccgcacc
     3601 tcgccttgtt gcttcgccaa tgtggggggc tgccgctcgc cctcgaattg gtcgccgccc
     3661 aactgtcggc gatgcccgtc ggcgacctgc tcgaccacct gtcggacgtg cttgtcgacg
     3721 gtgaggatcg gctgcgcgcc gtcgttcgcg gtagctacgc gttgctcgac gacgacgagg
     3781 cggcggtcta tcgacggttc gccgttctcg atggcccagt gggtctgccg ctcatccgcc
     3841 aggtggtctc gggcggcccg atcgtgcccg tgcgagtcgt gcgcatcttg cgcgagctga
     3901 ccgctcgcgg gttgctcgcc gtcgaccgaa gcggtgcgcg ctggcgctat caccaggacg
     3961 acgacctaca caggttcgcg cgcgagctgc tcgtcgacca cggtgaggag tcggccgcgt
     4021 tcgatcgcct cgccgatgcc ctgcgagccg cgttgccggc cgatgcgcgc gagacgccgg
     4081 cgtcatatcg cgaggccgtg accgacatgt tgggatcagt gcggtcgctg ttcggggccg
     4141 cgatcagtgg ccacgccgac cgcgaccgct gcctcgaact cgcgttccgc ctgcatcgct
     4201 actgggccgc aaccaacgtc gccgagggcc gttactggct gtcgcgactg ctcgccgagg
     4261 gcggtcgaag cgagtggact ccgtacgcga cctacgcgat gggttacctc gactattggt
     4321 cgggcgatac ggcgagtgcg atccacgaac tcgagaccgt ggtgcgacta ctcggtggag
     4381 tcgaggatcc ctataccgcc cacgccctga tctatctggc cggcctgctc gacgaccttg
     4441 atcgtggcgc ggaagcactc gagtacgtgc ggcaggcgat cgtcgccgcg cgtccgttca
     4501 gcaccgacct ccaggtaacc gctgcgatgg gcatgggcag cgtgctctcc gaacgcggag
     4561 aagccgatgc cgcgcgatat gcagccgacg cgattgcgct gtgcagagcg ggcggctcac
     4621 ccgagcagct agcggcggcg atgccgacgg cggcgatggt gtgctggcag gtgggggcgc
     4681 tcgacgaagc gcgcgcgtac gtcgcagagg ctcgccccat gcacagcgac agccgccgca
     4741 ttgcgagagt cgtgctgctg tcgacgtccg ccggactcgc ccttgccgaa ggcgacatag
     4801 atgcggcagt cgacttcggc gaggcggccg atcgcgaggc gaccgagcta ggcgtcgaac
     4861 gggaagtgcc gttgatccgg tcggttcttg cgcgggcgct gcttgctcgc ggggacgtcg
     4921 tcggtgcggc cgagcgcgcg atcgctgcct tcgacgccgc gttctcgatc acgttcgagt
     4981 tcccgcttgc gatctgcctc gagacggccg ccctcgttct gcgggccgcc cgggtcagcg
     5041 gcggggagtt ggctgagctc ctcgccagcg ctgaggtcat caggcagcgc ggggaccggc
     5101 cgccgccggc gccgcttgcc gccgacatcg agtcgctgcg cgctgacgtc ggggccgagc
     5161 tcctcgccgt ccggctgatc ggtgctcgtg aggcgggcac cctcgcggtg gccaagctgt
     5221 cggcgctgcc gcctcgacac gagtgacctc cgggagccgg tgtaacgggc ccgcactcca
     5281 tcgcggtcag gtaacgcctc ggtaatccgg cgaggccggc ggttcggtgg ttcgcgaaac
     5341 tacgtatctg cggggcccga tctaaggaga actcctcatg cgggctcggg gctagcgcgg
     5401 catcgtgaca tccaggagat tggtcgtcga tctcagccag ggatcaagca gcacaggaat
     5461 ctcagtgaac ctcagcgaat ggagacacga catgccgctt tacatggaca cccatcacaa
     5521 ggtcgacggg ttgacggccg aagccgtcgc cggtgcccac gcccgcgacc tggaggtcca
     5581 gcgcgcacac gacgtcaact atctccagta ctggttcaac gaggacaccg gcacggtgtt
     5641 ctgtctcgtc gaagctccga atccagaagc agcccacgcc gtccatcgtg aagcacacgg
     5701 cctcgtcgcc gacgagatca ccgaagttcg acccggcgcc taggtggccc gaatgtggaa
     5761 cactttgcgg gtgaccgagc agccgtccag cggcgcattt gtcggtcggg ttgccgaact
     5821 cgaccgactc gatgcggcgc tggccgcact cgcggagcgt ggcgccgcgg cggtcttggt
     5881 cggcggcgac gccggtatgg gcaagtcgcg gctgatcgaa gagttctgcg cgcgagcgcg
     5941 cagcgccggc actctcgtcg ccgtgggtgc ctgcacgccg gcagaaggcg gcgggcttcc
     6001 cttcggccca gtcgtcgggg tctt
//
LOCUS       DASWVF010000861         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_203260, whole genome shotgun sequence.
ACCESSION   DASWVF010000861 DASWVF010000000
VERSION     DASWVF010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203260"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VGD55_15240"
     CDS             complement(<1..1687)
                     /locus_tag="VGD55_15240"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY0871753.1"
                     /translation="MLLTSLTGRWALRIVGVGAAVGTVMASGLTLSTAAIAAPVAPAV
                     ASAALLPSVTLPSSVQRISGASRFDTAIATSQDEFAATGSAKAVVLTRADNYPDALAG
                     VPLAAKVGGPLLLTSSASLNPTVSAEIVRVLPAGGMVYILGGTAAVSAAVATTLTDNH
                     FVVKRLAGSDRFATAVSIADALGDPTTVFEATGLNFPDALAGGPAAIMSSAAILLTNG
                     SVQAPATTAYLAAHTGGTHYALGGPAAAADPSATSIVGTDRYATAARVADTFFTAPTV
                     VGVATGANFPDALAAGPDLGSKQAPLLLVPPAGALPEGSTRELILDNPTVRSALVFGG
                     TSSISDDVATQVGVLANLGATVASESASAEFSGQYGVLGTHLQTGSGSANTTQVFDGL
                     GGTSTFYTQGAGSKTIPSTPTRADFAAFPTTNPSALESAINTKFASVYAAEGYSSTAP
                     DAQFLVNAEQVLLNPLASPTLRVAVFAAVTRLTGTHTQSGVKDTTGRSGILVSGTISG
                     NSSLNGDLLGYLLDPTTGTLLEDNIVTPTGTTVAASTVTAFTTTTTAPADPYSS"
     gene            complement(1797..>2186)
                     /locus_tag="VGD55_15245"
     CDS             complement(1797..>2186)
                     /locus_tag="VGD55_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871754.1"
                     /translation="NSMFSTFDSDNSLTGTADDLFTINAEQVFLNPVSSPSLRLAVYA
                     TLAVDDEDTDETPGVKDSTGRVGIEIFVSPGADASDKSRISYIVDPNTWTPLEDTILD
                     TTGKVLGRTTVLSVTTTPTMPADPYST"
BASE COUNT          378 a          670 c          770 g          368 t
ORIGIN      
        1 aggacgagta cggatctgcc ggtgcggtcg tggtcgtggt gaacgcagtg acggtactgg
       61 ccgcgaccgt ggtgccggtt ggcgtgacga tgttgtcttc gagaagcgtg ccggtcgttg
      121 gatccagcag atacccgagc aggtcgccgt tgagtgaact gttgccgctg atggtccccg
      181 acacaagaat gccgctgcgc ccagtggtgt ctttgactcc cgactgagtg tgggtgccgg
      241 tcaatctggt aactgcggcg aagaccgcca cgcgcagtgt cggcgaggcg agcgggttca
      301 acagaacttg ctccgcgttg acgaggaact gcgcgtcagg agcggtcgat gagtaaccct
      361 cggccgcgta gacacttgcg aacttcgtgt tgatcgcgct ctcgagtgcg ctcgggttgg
      421 tggttgggaa cgctgcgaag tcggcccgcg tcggcgtgct ggggatcgtc ttcgaacccg
      481 cgccttgggt atagaaggtc gaggtcccgc caaggccatc gaatacctgc gtggtgttgg
      541 ccgaacccga gcccgtctgc agatgcgtac ccaggacgcc gtactgaccg ctgaattcgg
      601 cggacgccga ctcgctcgcc accgtcgccc cgagatttgc caggaccccg acttgcgtgg
      661 cgacgtcgtc tgagatcgac gacgtgccgc cgaagaccaa ggcactgcga acggtcgggt
      721 tgtccaagat cagttcgcgg gtcgagcctt cgggcagcgc ccccgccggc ggaacgagaa
      781 gtagtggcgc ctgcttcgaa ccaaggtcgg gcccggcagc gagcgcgtcg gggaagttag
      841 ctcctgtcgc aaccccgaca acggtcgggg cggtgaagaa ggtgtcggct acgcgagcag
      901 ccgttgcgta acgatcggtg ccgacaatcg aggtggcact ggggtcggcc gcggcagccg
      961 ggccgccaag ggcgtagtgg gtaccgcccg tgtgcgcggc gaggtaggca gtcgtcgcag
     1021 gcgcctgaac cgagccgttg gtcagcaaga tcgccgcgct cgacatgatc gcggccggcc
     1081 cgcctgcgag cgcgtcaggg aagttcaggc cggtcgcctc gaacacggtg gtcgggtcac
     1141 cgagggcgtc ggcgatcgag acggcggtcg cgaatcggtc gctgcctgca aggcgcttca
     1201 ccacgaagtg gttgtcggtg agggtcgtag caactgcggc gctcaccgcc gccgtcccgc
     1261 caaggatgta caccatgcca ccggctggca gcactcgcac aatttcggcg ctgaccgtcg
     1321 gattcaacga cgctgacgac gtgagtagca gtgggccgcc gaccttggca gcgaggggta
     1381 cgccggccag cgcgtcggga tagttatcgg cgcgggtcag aacgacagcc ttcgcgctgc
     1441 cggtcgcggc gaactcgtcc tgcgaggtcg cgatcgcggt gtcgaagcgg cttgcccccg
     1501 aaattcgctg cacggacgac ggcaaggtga ccgacggcag tagggccgcc gaagcgactg
     1561 ctggcgccac cggcgcggcg atcgccgccg ttgagagcgt cagcccgcta gccatcacag
     1621 tgcctactgc ggcgcccaca ccaacgatgc gaagcgccca ccggccggtc agggacgtca
     1681 aaagcatgag cagcatcctc cgcgtcggcg gctggcgcca cccggcgacg aggctatacc
     1741 gaggcagcgg cccgggcgct cgtatgcgcc cgggccgctg tctgacacgc gtgcgcttag
     1801 gtgctgtacg gatccgcagg catcgttggg gtggtcgtca ccgacaagac cgtcgtgcgc
     1861 ccgaggacct tgcccgtcgt atcgagaatg gtgtcctcga gcggggtcca cgtgttgggg
     1921 tcgacgatgt aggaaatccg gctcttgtca gaagcgtcag cccccgggct gacgaaaatc
     1981 tcgataccga cccgtccggt tgaatccttg acaccaggag tctcatcggt gtcttcgtca
     2041 tcgaccgcga gcgtcgcata aacggccaac cgcaacgacg gcgacgagac cggattgagg
     2101 aatacctgct cggcattgat cgtgaataga tcgtcggccg tgccggtgag gctgttgtcg
     2161 gagtcgaacg tgctgaacat cgagtt
//
LOCUS       DASWVF010000862         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_228963, whole genome shotgun sequence.
ACCESSION   DASWVF010000862 DASWVF010000000
VERSION     DASWVF010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228963"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VGD55_15250"
     CDS             <1..1707
                     /locus_tag="VGD55_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0871755.1"
                     /translation="TADAVTESPAATTVTGPAEHPARTLLRISFGLLWIFDGLLQIQQ
                     AMPVGLPTQVVQPAAATSPGWVKHLVNDGTLIWTRHPISAAAATVWIQIGVGVLLLLD
                     RCGRLSQWAGLVGVAWGLVVWVFGEAFGGIFGSGQSWLFGAPGAVLFYVAAGALVALP
                     ERVWNDKRLGRALLGVLGIFFIGMAILQAWPGRGFWQGQRDPHATAGSLTSMVQTMSQ
                     TSQPRALSSLVSSFASFDAQHGWFVNLFVVLALAGLGIALLISRERVTFGALVFGSIF
                     CIAVWVFVQDLGFLGGVGTDPNSMMPTVLLIAAGYLALTRRVEPALADETVPAEPAAG
                     AAGGETPAGSMRRLRPLSSPATLGLISVVATFGVVLVGAVPMAFASVNTHTDTIVTEA
                     INGQPQTTDTRAAPFELTDQHGTPVNLATFKGRTVALTFLDPVCTTDCPLIAQEFRQV
                     DQRLGTSPNTAFVAVVANPTYLDVQFVQAFDAQENMDMPNWYFLTGSLAQLRAVWDSY
                     GIQVQTTGSGGMSAHSDTTYVIDGNGHLRTILGADPGDTSTDSASFATLLNKEMRAVA
                     GA"
BASE COUNT          278 a          560 c          561 g          345 t
ORIGIN      
        1 accgccgatg ccgtcaccga atcccccgcc gccacgaccg tgacaggccc cgccgaacac
       61 cctgcgcgca ccctgctgcg tatcagtttc ggactgttgt ggatcttcga cgggctgctc
      121 caaatccagc aagcgatgcc ggtcgggttg ccgacgcagg tcgtgcagcc ggccgcggcg
      181 acctcgcccg gttgggtgaa gcacctggtc aacgacggca ccttgatctg gacgcggcac
      241 ccgatttcgg ctgctgctgc gacggtttgg atccagatcg gcgtcggggt gctgctgctg
      301 ttggacaggt gcggtcggct ctcgcaatgg gccggtctcg tcggcgttgc ctggggattg
      361 gtggtctggg tcttcggcga ggcgttcggt ggcatcttcg gttctggcca gagctggctg
      421 ttcggtgctc cgggcgcggt gctcttttat gtcgcggccg gcgcgttggt agcgttgcct
      481 gagcgggtct ggaatgacaa gcgactaggg cgggctctgc tcggcgtcct cggaattttc
      541 ttcataggta tggcaattct tcaagcgtgg ccaggccgag gattttggca ggggcagcga
      601 gatccgcacg cgaccgcagg cagtctcacg tcgatggtcc agacgatgtc gcagacatcg
      661 cagccgcgtg cgctgtcgtc gctggtgagt tcgttcgcgt cgttcgatgc gcaacacggc
      721 tggttcgtga acctgttcgt cgtgctcgcg ctcgcagggc taggcatcgc gcttttgatc
      781 agtcgcgaac gtgtgacgtt tggcgcgttg gtgttcggca gcattttctg catcgccgta
      841 tgggttttcg tgcaggacct cggatttctc ggcggcgtcg gcaccgatcc gaacagcatg
      901 atgccgaccg tgctgctgat cgccgctggc tatctggcgc tcacccgccg ggtcgaacct
      961 gcgctcgctg acgaaacagt gcccgccgaa ccggcggccg gcgcggcggg gggcgaaacg
     1021 cccgcgggca gtatgcgacg gctgcggcca cttagctccc ccgccaccct cggcctgatc
     1081 tccgtggtcg cgacgttcgg tgtcgtgctc gtcggtgcgg tgccgatggc gttcgcgtcg
     1141 gtgaacacgc acaccgacac catcgtgacc gaagccatca acggccaacc ccagacgact
     1201 gacacacgcg ccgcaccgtt cgaattgacc gaccagcacg gcactccggt caatctcgcg
     1261 acgttcaaag gacgcacggt tgcgctgact tttctcgacc ctgtctgcac aacggactgt
     1321 ccgctgatcg cccaggagtt ccgtcaggtc gatcagcgcc tcggcacatc accgaacaca
     1381 gcgttcgttg cggtggtcgc gaacccgacc tatctcgacg tgcagttcgt gcaggccttc
     1441 gacgcgcaag agaatatgga catgccgaac tggtatttcc ttaccgggtc gctcgcacaa
     1501 ttgcgcgcgg tgtgggactc ctacggcatt caagtgcaga ccactggcag cggcggcatg
     1561 tccgcgcaca gcgacaccac ctacgtcatc gacggcaatg gccacctgcg cacgatcctc
     1621 ggcgccgacc ccggcgatac ctccaccgat tcggcatcgt tcgcaacgct gttgaacaaa
     1681 gaaatgcgtg cggtcgcggg cgcatgagat cgcggcaaca cgcagtcgac attcgaaagc
     1741 agcc
//
LOCUS       DASWVF010000863         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_177268, whole genome shotgun sequence.
ACCESSION   DASWVF010000863 DASWVF010000000
VERSION     DASWVF010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177268"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VGD55_15255"
     CDS             <1..593
                     /locus_tag="VGD55_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871756.1"
                     /translation="VVTYTAAAGVTPFVKAEATVAAAGVTGGGTGIDTNNTLDDAIPG
                     GVMKLTKSFSITSTNCPAGASPGPPANTVCPGGIIQYSIAYVNTVPAAMTTNIGTESS
                     LTWAFAATSTQAGTFLITDDGAVGNWSTYTFGLNAAPSDTTASTTYAYTGSAFASGTY
                     PSMTAGYSKFVATVGGSSFQCVPGTSGTITYNVTVK"
     gene            597..2804
                     /locus_tag="VGD55_15260"
     CDS             597..2804
                     /locus_tag="VGD55_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871757.1"
                     /translation="MSRFWTRLTASLLFVLSLQESSFAVTLGGTQIQSTASATFQTSS
                     AASSSIASNEVTTVVASIGSIAVSPKENGCDPQADVFPIGPLLVKTFSITNNSDVVDA
                     YTVQTATTSAGSIAGVAFVNASGTTTPATVGQTLSQTIQPGASLQVQVSIVTTAIAVG
                     THVEVSLGARTSVMATINGLQSDSGQACGIASAGASFGAPQGSTALLALLINGQPAIQ
                     TSPGLTVTYSLTFENFGAIAATNVMLTDNLPADVQADPSSVKFDGTLHSGASLSTQAV
                     RTAQATSSLVAPNSGQVLSIPLGSIPPGIPETVQFTATVGATVVPGTTYLSNATLIAD
                     NAASILTQPAGLFVGTANLVYDGAVGQTHPIANATIQIIDPSLSAALMQPVGPAIAPN
                     TQNTNPFITGPSGMYSFGLSPTQYGSNGTPVTYGVLVTAPGYLNRKLSLTLVPNSVNN
                     LYSATIAALDGQPVASAGAFSLTTGAVSISDVYGFFGNFPLFTTQAVTLTKTADRNVA
                     SAGDRIVFTLQFSGNGPTTLGTTTVVDTLPTGLVYASGTGRLDSAPNEPSVNGRTLTW
                     TLSSLTASHSITYATVIMPSVATQATLVNTASISAAFPLSPGTFATASANTSVQVVAG
                     VFSDRIVITGRVFLDDRGTGHFATGDSGVAGVRVYLEDGESVVTDSFGRYSFPAARPG
                     MHVLRIDKTTFPTHVGAYADLAYDSERSIRRLVHGLFDSGLMEDVNFALRRTP"
BASE COUNT          576 a          978 c          844 g          540 t
ORIGIN      
        1 acgtcgtgac gtacacggcg gctgccggcg tgacgccgtt cgtgaaagct gaagccaccg
       61 tggccgcagc cggggtgacc ggcggcggaa cgggtatcga caccaacaac acacttgacg
      121 acgccatccc cggcggcgtc atgaagctca ccaagtcgtt ctcaatcacc agcaccaact
      181 gccccgcggg cgcatctccc gggccgccgg ccaacacggt ctgcccgggc ggcatcatcc
      241 aatactcgat cgcttacgtg aacacggtcc cggctgcgat gacgaccaac atcggaaccg
      301 aatcgtcgtt gacgtgggcg ttcgccgcga cctcgaccca agcgggcacg ttcctcatca
      361 ccgatgacgg tgcggtcggc aattggtcga cgtacacgtt cggcctcaac gccgctccct
      421 cggacacgac cgccagcacg acctacgcgt acaccggaag tgcgttcgct agcggcacct
      481 atccctcgat gacggcgggc tacagcaagt tcgtcgctac ggtcggggga tcgtcattcc
      541 aatgcgtgcc gggcacgtcg ggaacgatca cctataacgt gaccgtgaag taaaacttgt
      601 cacgtttttg gacccgtctt actgcatcgc tgctcttcgt actctctctc caggagagca
      661 gctttgccgt aaccctcgga ggcacccaaa tccaatcgac ggcatcggcg acctttcaaa
      721 cgtcgagtgc cgcctcgagc agtatcgcct cgaacgaagt gacgacggtc gttgcttcga
      781 tcggttcgat cgcggtcagt cccaaggaga acggttgcga cccgcaagct gacgtgttcc
      841 cgatcgggcc gctgctcgtt aagacgttta gcatcaccaa caacagcgac gtcgtcgacg
      901 cctacaccgt tcagaccgcc acgacgagcg cgggatcgat tgccggcgtc gcgttcgtca
      961 acgcaagcgg aacgacaacc ccggcaacgg tcggacaaac gctttcgcaa acgattcaac
     1021 ccggcgcttc gctccaagtt caggtctcga ttgtcaccac ggcaatcgcg gtcggtacac
     1081 acgtagaggt ttcactgggt gcacgcacga gcgtgatggc gacgatcaat ggtctgcaaa
     1141 gcgattcggg acaagcgtgc ggaatcgcgt cggccggcgc gtcgttcggc gcaccgcaag
     1201 gttcgactgc gctgctcgcg ctgctcatta acggccaacc cgcaattcag acctcaccag
     1261 gcctgacggt aacgtactcg ctcacctttg aaaacttcgg cgccatcgcc gcgacgaacg
     1321 tgatgctcac ggacaatctg cccgccgacg tacaggcgga tccgagcagc gtcaagttcg
     1381 acggcacact ccactccggt gcatcgctct cgacgcaggc cgtgcggacc gcgcaggcca
     1441 caagctcgct agtcgcaccc aatagcgggc aagttttgag cattccgctt ggatcgatcc
     1501 cgccgggcat cccggagacc gtgcagttta ccgctacggt cggcgcgacg gtcgtgccgg
     1561 gcaccacgta tttaagcaat gcgacgctga tcgccgacaa cgccgcgagc attctcaccc
     1621 agccggccgg ccttttcgtt ggaacggcaa atctcgtata tgacggagcc gttggacaga
     1681 cgcaccccat cgccaacgcg actattcaaa tcatcgatcc gtcgctgagc gcggcgctca
     1741 tgcagccggt cggtccggcg atcgctccca acacgcagaa caccaacccg ttcatcaccg
     1801 gtcccagcgg catgtactcg ttcggcttga gcccgacgca gtacggaagc aatggaacgc
     1861 ccgtgacgta cggcgttctc gtcacggcac cgggctacct caatcgtaag ctctctctga
     1921 cactcgtgcc gaacagtgtg aacaatctgt acagcgcgac gatcgccgca cttgatgggc
     1981 agcccgtcgc tagcgcaggc gccttctcgt tgactacggg cgcggtctcg atttcagatg
     2041 tctacgggtt ctttggaaac ttcccgctgt tcacgacgca ggccgtcacc ttaactaaga
     2101 ccgccgatcg caacgtcgcg agcgccggcg atcgtatcgt tttcaccctt caattctccg
     2161 ggaacggacc gacgacacta ggcacaacga cggtcgtgga tacactgccc accggtttag
     2221 tctacgcctc aggtacggga cgtctcgata gtgcaccgaa cgaaccgtcg gttaacggcc
     2281 ggacgctcac ctggacgtta tcgtccctga cggcctcgca ttcgatcacc tatgcgactg
     2341 tcatcatgcc gagcgtcgcg acgcaagcga cgcttgtgaa taccgcgtcg atcagtgccg
     2401 cgttcccctt gtctccagga actttcgcca cggcgagcgc gaacacgagc gtgcaagtcg
     2461 ttgcaggcgt ctttagcgat cgcatcgtca tcaccggacg agtcttcctc gacgatcgcg
     2521 gaacgggaca cttcgctacc ggtgacagcg gagtggccgg cgtgcgcgtg tacctcgaag
     2581 acggcgaatc agtagtcacc gattcgttcg gccgctacag cttcccggcg gcgcgtcccg
     2641 gtatgcacgt gctgcgcatc gacaaaacga cgttcccgac gcatgtcgga gcgtacgccg
     2701 atctcgcgta tgatagcgag cgcagcatcc gtcgcctagt ccacgggcta ttcgatagcg
     2761 gcctgatgga agacgtgaac tttgcgctgc ggagaactcc gtgaagcgcc ttctagcgag
     2821 catcttagtg atcgcgatga tctcggcgtc cgcgccggga gcgctggcgg acggccttcc
     2881 gggtatcacg attctgcgtc cggcgggcgc gccgcctgcg ccgacgatgc cccctgcg
//
LOCUS       DASWVF010000864         5155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_356266, whole genome shotgun sequence.
ACCESSION   DASWVF010000864 DASWVF010000000
VERSION     DASWVF010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356266"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGD55_15265"
     CDS             <1..346
                     /locus_tag="VGD55_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0871758.1"
                     /translation="LRLVALEALARSSELHHYVAGQTLMTQGERGDEFHVVVKGRVSV
                     VRDGVAISELDAPVGVGELALLYGIPRTATVLAIDGVETIGVDREAFLLALDHQPPTA
                     AWQALVEARLGR"
     gene            349..612
                     /locus_tag="VGD55_15270"
     CDS             349..612
                     /locus_tag="VGD55_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871759.1"
                     /translation="MFGRKKLLVLGGFAVALGLAIAVYASFNAGNTYQKFKHHCLAGA
                     GASVVQLSVSTHAQYMGGPATTYTMGCRQPDGSVTATAKTNQP"
     gene            complement(754..2373)
                     /locus_tag="VGD55_15275"
     CDS             complement(754..2373)
                     /locus_tag="VGD55_15275"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY0871760.1"
                     /translation="MAATSVELPPTPTPPAAATNPLRDRLVPTFPGSRLWGWLAPLAI
                     TLFAGYLRFNRLAVPGGTGSTLNAVCKDKPGPQACVFDEVYYTHDAWSLFHHGVELNV
                     TNNGPGYIVHPPLGKWMIALGEWICGDPNFTSTFGWRFMSAIVGTLAVLIVARTARRM
                     TRSNLLGCIAGLIFSLDGLEYVQSRVAMLDIFLMFWVVVAFACLILDRDQVRARLADR
                     AITTAYGDYGPRVGMRWWLLACGVSSGAAVATKWDGAALIPTFAFLAFIWTIGAKRAI
                     GVKKPFMSTLRRDLVPTGAWMVIVSIGVYIASWTGWFLNGPKYAYDREWAKGRHSDWS
                     FIPESVRSLWHYHWEIYEFHKHLSSYHSYRENAWGWLFDARPVLYYAGYPHPPVSGCH
                     TGGLGCARMIYDLGTPAIWWASIPVMCFMVYIICKRDWRGTAILVPFLILYLPWLLQF
                     KRTMFFFYALPLLPFISIALAVTIGYLIGPATASFARRRAGTIAGGTYVLIVVAMFFY
                     FWPILSARTVPFVGGWVDRMWFHSWIESNGS"
     gene            2397..3281
                     /gene="rsmI"
                     /locus_tag="VGD55_15280"
     CDS             2397..3281
                     /gene="rsmI"
                     /locus_tag="VGD55_15280"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0871761.1"
                     /translation="MENSGTGPDSPPPAGLLVLAATPLGNPGDASARLRAELETADVV
                     AAEDTRRLRRLTADLGVRPVGRIVSYFEANETARTDELMADLLAGRRVVVVSDAGMPS
                     ISDPGYRLVAAAIAAGIDVTCLPGPSAVTTALALSGLPVDRFCFEGFLPRRGGARDSR
                     LRELAGEPRTMVFFEAPHRLAESLASLASAFGPTRSAAVCRELTKTYEEVRRGSLDEL
                     VEWAAVGVRGEITLVVAGTPDRAEAALSASSADLADLVAAGEDSGMSRKEAITAVAAA
                     TGANRREVFDALVARKYS"
     gene            3341..4057
                     /locus_tag="VGD55_15285"
     CDS             3341..4057
                     /locus_tag="VGD55_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871762.1"
                     /translation="MRQGFASGLRAEIGRLRLVVVRRWSARGALGYLLTVVVVTLIVV
                     MWALDHTTGRTFVAACCGERVGSPITTALLKLPGSLVAPAALLPVWGSAVQVVLAFGL
                     AEAAVGALRTLAVAATSHVVATMAGRYFVWYAPIALGGLAPSWRSALDTGPSAATVGV
                     AVYLAIVLGCGRIGGVVVLAVAITFAARSDLAGREHLVAMVVGAGCAAVQLLAYRWSA
                     RTAWPELAIRDVDREPSILG"
     gene            4105..>5155
                     /gene="metG"
                     /locus_tag="VGD55_15290"
     CDS             4105..>5155
                     /gene="metG"
                     /locus_tag="VGD55_15290"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0871763.1"
                     /translation="MTTPPAAKPFYLTTPIYYVNDVPHIGHAYTTVAGDVLTRWHRQR
                     GEQVWYLTGTDEHGEKVLRSAQAHGVSPQQWADRLVEDEWRPVWRSLDIANDDFIRTT
                     EARHTERVQAFLQGLYDAGEIYEGLYEGPYCVNCEEFKLRSELLDGTGEYLGQKVCPI
                     HGRPVEELSETNYFFRLSKYGDALLAHYAEHPDAVEPESARNEVLSFIRSGLQDLSIS
                     RSSFDWGIKVPWDDTQVVYVWFDALLNYATAVGLDAPDAVDQAMFAKTWPADVHLVGK
                     DILRFHAVIWPAMLLAAGVALPRKVFAHGWLLVGGEKMSKTKLTGIAPAQITDHFGSD
                     AFRYYFLRAIQFGQDG"
BASE COUNT          874 a         1651 c         1738 g          892 t
ORIGIN      
        1 cctgcgactc gtcgcactcg aagcgttggc gaggagcagc gagttgcatc actacgttgc
       61 agggcaaacg ctgatgacgc agggcgagcg tggcgatgag ttccacgtcg tggtcaaggg
      121 gcgtgtcagc gtcgtccgcg acggggtcgc gatctccgag ctcgatgcgc cggtcggtgt
      181 cggtgagctg gctctcctgt atggcattcc ccggacggcc acggtgctgg cgatcgacgg
      241 cgtcgagacg atcggcgtcg accgagaggc cttcttgctg gcgctggacc atcagccccc
      301 gaccgccgca tggcaggctc ttgtggaggc ccgcctcggg agatgatcat gttcggccgg
      361 aagaagctgc tcgtgctcgg cgggtttgcc gtcgccctcg ggctggcgat tgcggtctac
      421 gcgagcttta acgccggcaa cacgtaccag aagttcaaac accattgcct cgcaggcgcg
      481 ggtgcctcgg tagtgcagct cagcgtttca acacacgcgc agtacatggg cggcccggcg
      541 acgacctaca cgatgggttg tcggcagcca gacggttcgg tgaccgccac ggcgaagacg
      601 aaccagccgt gagcacctct agaagccagc cgtccagacc attgatgcca gtggccaggg
      661 accgttacga tcgcggcgcg gcgtccggcc ctcctccccc gtagggtcgg gcgccgcgtt
      721 acgtggtgac ctggggcgaa cccgagcagg ccgctacgaa ccgttgctct cgatccagct
      781 gtggaaccac atccggtcga cccagccgcc gacgaagggc acggttctgg cgctgaggat
      841 cggccagaag tagaagaaca tcgcgaccac gatcaggaca tacgtccccc ctgcgatcgt
      901 gccggctctg cgtcgggcga acgatgcggt cgccgggcca atgagatagc cgatcgtgac
      961 cgcgagcgcg atgctgatga acggcagcaa cggcaacgcg tagaagaaga acatcgtgcg
     1021 cttgaactgg agcaaccacg gcaggtaaag gatcaggaac ggcaccagga tcgcggtgcc
     1081 tcgccagtcg cgcttgcaga tgatgtagac catgaagcac atgaccggga tcgacgccca
     1141 ccagatcgcc ggcgttccga ggtcgtagat cattcgtgcg caaccgagcc caccggtgtg
     1201 gcaacccgac accggcgggt gcgggtagcc cgcgtagtag agcaccggtc gcgcatcgaa
     1261 gagccagccc cacgcattct cgcggtagga gtggtacgag ctgaggtgct tgtggaactc
     1321 gtagatctcc cagtggtagt gccaaagact gcgcaccgat tcggggatga acgaccagtc
     1381 ggaatgccgg cccttggccc actcccggtc gtaggcgtac ttcggaccgt tcaagaacca
     1441 gcccgtccac gatgcgatgt agacaccgat cgagacgatc accatccacg ccccggtcgg
     1501 aacgaggtcg cggcgaagcg tcgacatgaa aggtttcttg acgccgatcg cgcgcttggc
     1561 tccgatggtc cagatgaagg caaggaatgc gaacgtcggg atcagcgccg cgccgtccca
     1621 cttcgtcgcg acagctgcac ccgaactgac gccgcacgcg agcagccacc accgcatgcc
     1681 gacacgtggg ccgtagtcgc cgtacgcggt ggttatcgcc cgatcggcca gcctcgcgcg
     1741 cacctgatct cggtcaagaa tcaggcaggc gaatgccacg accacccaga acatcaagaa
     1801 gatgtcgagc atcgcgaccc ggctctgcac gtactcgagc ccgtcgaggc tgaagatcag
     1861 cccggcgatg caaccgagca ggttcgagcg cgtcatgcgg cgggcagtgc gcgcgacgat
     1921 caacaccgcg agggtgccga cgatcgcgga catgaagcgc cacccgaacg tgctcgtgaa
     1981 gttggggtcg ccgcagatcc actcgccgag cgcgatcatc cacttgccga gcggcggatg
     2041 cacgatgtag ccgggcccgt tgttcgtgac gttcagttcg acgccgtggt ggaacaggct
     2101 ccatgcgtcg tgcgtgtagt agacctcgtc gaagacgcac gcctgcgggc caggcttgtc
     2161 cttgcagacg gcgttgagag tcgagcccgt accgccaggc accgcaagcc ggttgaaccg
     2221 caggtagccc gcgaagagcg tgatcgcgag cggcgcgagc catccccaca accgactgcc
     2281 ggggaacgtc ggaacgagcc ggtcgcgcaa tggattcgtc gcagccgccg gcggcgtcgg
     2341 cgtcggcggt agctcgacgg aggtggcagc cacctcgcca tcgtatggtg gcggccatgg
     2401 agaactccgg caccggtccg gactccccgc cgcccgcagg tttgttggta ctcgcggcca
     2461 ctccgttggg caatcccggc gatgcctcgg cccgattgcg tgcggagctc gaaacggctg
     2521 atgttgtcgc cgcggaagac acccgccggc tgcgacggct gacggctgac ctcggcgtcc
     2581 ggcccgttgg acgcatcgtt tcgtacttcg aagccaacga gaccgcacgg accgacgagc
     2641 tgatggcaga tcttttggcc ggacgccggg tggtggtcgt cagcgacgcc ggcatgccgt
     2701 cgatctccga cccgggatac cgcctggtgg cggccgcaat cgcggccggc atcgacgtga
     2761 catgcctgcc tggcccgtcg gcggtcacca cagcgttggc gttatccggg ttgccggtcg
     2821 accggttctg cttcgaggga ttccttcccc gccggggcgg cgctcgcgac tcccgcttgc
     2881 gcgaactcgc gggcgagccg cgcacgatgg tgttcttcga ggcaccgcat cggctcgccg
     2941 aatcgctggc gtcgctggcg tcggcattcg gcccgacccg gtcggcggcg gtgtgccgtg
     3001 aactcacaaa gacctatgaa gaggtgcgcc gaggatcgct tgacgagctc gtcgaatggg
     3061 cggcagtagg ggttcgcggc gagatcacgc tcgtcgtcgc cggtacgccc gaccgtgctg
     3121 aggccgcgct gagtgcgagc agcgccgatt tagcggatct ggtcgccgcc ggtgaggatt
     3181 ccggcatgtc gcggaaggag gcgatcacgg cagtggccgc cgccaccggc gcgaatcgcc
     3241 gggaggtctt cgatgctttg gtggcgcgca aatactcctg agacggcgcg ccgaatcggg
     3301 tggcatgatc gatgctgttc gtcaggtaac ggggagtgtg atgaggcaag ggttcgcgtc
     3361 cggactgcgc gcggagatag ggcggcttcg cctcgtcgtt gtgcgtcgct ggagcgcgcg
     3421 cggagccctt ggttacctgc tgaccgtcgt ggtcgtcacg ctcatcgttg tcatgtgggc
     3481 cctcgaccac accacagggc ggacgtttgt cgcggcatgc tgtggtgaac gcgtcggttc
     3541 cccgatcacc acagcgttgt tgaagctgcc tggatcgctc gtggcgcccg ccgctttgtt
     3601 accggtgtgg ggttcggcag ttcaggtcgt ccttgcattc gggctcgccg aagcggccgt
     3661 cggggcattg cggacccttg ccgtggcagc cacgtctcac gtcgtggcaa caatggccgg
     3721 tcgctatttc gtttggtacg cccccattgc actcggtggg ctcgcaccgt cgtggcgcag
     3781 cgccctcgac accggtccgt cggcagcgac ggtcggcgtc gcggtctatc tcgccatcgt
     3841 gctcggttgc ggccggatcg gtggggttgt ggtcctggcg gtcgccatca cgttcgctgc
     3901 ccgaagcgat ctcgcgggcc gcgagcacct ggtcgcgatg gtcgtcggcg caggctgcgc
     3961 tgccgtccag ctactcgcat accggtggtc cgcccgtacc gcgtggccgg aactggcgat
     4021 ccgggacgtc gatcgcgagc cgagcatcct cgggtaaggc ttggtcgccc ggcttacttt
     4081 gcgtcggtcg gtaaccttga tcgcgtgacc acccccccgg ctgccaagcc cttctacctg
     4141 accacgccga tctattacgt gaacgacgtg ccgcacatcg gccacgccta cacgacggtg
     4201 gccggcgacg tcttgacgcg ttggcatcgg cagcggggcg agcaggtctg gtacctcacg
     4261 ggcaccgacg agcacggtga gaaggtgttg cggtcggcgc aagcacacgg ggtctcaccg
     4321 cagcagtggg ccgaccggct ggtcgaagac gaatggcgac cggtctggcg ctccctcgac
     4381 atcgccaacg acgatttcat ccggacgacg gaggcgcgcc acaccgagcg cgtgcaggcg
     4441 ttcctgcaag gcctctacga cgccggcgag atctatgaag gcctctacga aggcccttac
     4501 tgcgtgaact gcgaggagtt caagctgcgc agtgagttgc tcgacggaac cggcgagtat
     4561 ctgggtcaga aggtatgccc gatccacggc cgtccggtcg aagaactcag tgaaacgaac
     4621 tacttcttcc ggctgtcgaa gtacggcgac gcgttgctcg cgcactacgc cgagcaccct
     4681 gatgccgtcg agccggagag cgcgcgcaac gaggtgctgt cgttcatccg gtcgggtttg
     4741 caagacctct cgatctcacg atcgtcgttc gactggggca tcaaggttcc gtgggacgac
     4801 acccaagtcg tctacgtgtg gttcgacgcg ttgctcaact acgcgaccgc cgtcgggctc
     4861 gatgcgcccg atgctgtaga ccaggccatg ttcgcgaaga cctggcccgc cgacgtccat
     4921 cttgtcggta aagacatctt gcgctttcac gccgtgatct ggcccgcgat gctgctcgct
     4981 gccggagttg ccttaccccg caaggttttt gcgcacggct ggctgctcgt aggcggggag
     5041 aagatgagca agacgaagct gaccggaatc gcgcccgcgc agatcaccga ccacttcggg
     5101 tctgacgcgt ttcgctacta cttcttgcgc gcgatccagt tcggccagga cggct
//
LOCUS       DASWVF010000865         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_177326, whole genome shotgun sequence.
ACCESSION   DASWVF010000865 DASWVF010000000
VERSION     DASWVF010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177326"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1088)
                     /gene="trbG"
                     /locus_tag="VGD55_15295"
     CDS             complement(135..1088)
                     /gene="trbG"
                     /locus_tag="VGD55_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEY0871764.1"
                     /translation="MHNRSSILFVAATIGCASRQPPPQYVRATPEKDPPRQPVVVEVP
                     QPMPMPGQLRRIPVHDVKRPEKQSPSDVIADANRKAALGPDREGYFNAIMTYDFADGA
                     LYQVYAAPLRLTTIELQPGEKIVGRPAAGDTVRWIMDVGRSGTGPAEQEHLYIKPTRS
                     ALSTTMVVTTDRRTYYLELHSFEDTYVAAVRWRYPQDDIAQLQAGAAREDALARSTTA
                     TNVNVDALNFNYRIGVEKGRPTWVPLQVFDDNHKTFIRFPATMLNRDAPALFVVSSAN
                     EAQIVNYRVKNDYYVVDRLFERAELRVGQQEQEIVQIVRSR"
     gene            complement(1131..1823)
                     /gene="trbF"
                     /locus_tag="VGD55_15300"
     CDS             complement(1131..1823)
                     /gene="trbF"
                     /locus_tag="VGD55_15300"
                     /inference="COORDINATES: protein motif:HMM:NF010446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HEY0871765.1"
                     /translation="MDPIANKRWKPEGEVETRYRRARQEWDRRMGTALAQAYNWRLAA
                     LLALGLVFVALLATVYLGVQPKAVPHIVQIDRIGAPTYLGPVGQSAREWKPTDAVLKY
                     HLRRFITDTRTVSSDVVVMKRYWTDAYTLITGSAANHLTAFAQETNPIKRGEDERVTV
                     DVKSLVQLSADTWQVDWVEKGWDKGGEETTSTIWRGVFRVLVRVPETDEQLATNPIGL
                     FIDEFHWSKVQG"
     gene            2258..2656
                     /locus_tag="VGD55_15305"
     CDS             2258..2656
                     /locus_tag="VGD55_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4087 domain-containing protein"
                     /protein_id="HEY0871766.1"
                     /translation="MTVTSRAIQAAAMWCVVTAGFSSNAADAPRVEKRCGWVSNPTPG
                     NWSLIDKDGEWAIAAQGEEGALDGLPEDRPSGRHWWVSTNSGGYGYGCMSLQVAVDKQ
                     TKRVLKIEGGKSLPLTTCRKDRAIRKLEPR"
     gene            complement(2698..3834)
                     /gene="trbL"
                     /locus_tag="VGD55_15310"
     CDS             complement(2698..3834)
                     /gene="trbL"
                     /locus_tag="VGD55_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEY0871767.1"
                     /translation="MEFNTLTTTLTTFVGVFQAGYSRLGPTINGLLAILAGIDIVLVG
                     FWWALGGGERLTEVIKKILFLGFWLWFTKSFQSNAKAFVDSLINAGLTAGGHPGGVNL
                     LLDPSRIAAYGLTATEQLAKALDDISVTDLGDVIIFGISYLLIMAAFLIMAIQAFLAV
                     LEYYVILAVAGILIPWALLPQTKFLAEKAIGAVVSAGIKLMVLAFIIAVVDPVLASIH
                     FSGPEIKLNELWSVLLTAAAIAFLTWHVPSLAAGLLAGSPSLSAAGVAQNVTSGSLLA
                     AGVAGGMVAATRAAVSGASALGQRAAYGAGVLFGGGSKGTQQGGGAIDTALGAGTSAA
                     ASAGRSAVEGFKARLSRASAPFRDSFRAGAERSANVHRHEPPKT"
BASE COUNT          667 a         1228 c         1347 g          707 t
ORIGIN      
        1 gagccgaagc cggggatcgt cggcgggtat cttgctctgg tggacatccg tgccctgtgt
       61 gacctctacc gaggtcggga catcgcttgg gttccgcgca gtgactgtgg tgtcagcgtc
      121 gatgtcgtta ggcatcagcg actccggacg atctggacga tttcttgctc ctgctggccg
      181 acccggagct ccgcgcgttc gaacagccga tccacgacgt agtagtcgtt cttgacccgg
      241 tagttcacga tctgagcctc gttcgcgctg gagacgacga agagcgcggg tgcgtcgcgg
      301 ttcagcatgg tcgccgggaa acggatgaag gtcttgtggt tgtcgtcgaa cacctgcagc
      361 ggaacccatg tcggccggcc cttctcgacg ccgatccggt agttgaagtt caacgcgtcg
      421 acgttgacgt tcgtcgccgt cgtcgaacgt gcgagagcgt cttcgcgggc agccccggcc
      481 tgaagttggg cgatatcgtc ctgcgggtat cgccacctga cagccgccac gtaggtgtcc
      541 tcgaacgagt ggagctcgag gtagtacgtg cgtcggtcag tggtcacgac catcgtcgtg
      601 ctgagcgcgg agcgggtcgg ctttatgtag aggtgctcct gctccgcggg cccagtcccc
      661 gaccgaccaa cgtccatgat ccaccggacc gtgtcgccgg cggccggacg cccgacaatc
      721 ttctcgcctg gctgcaactc gattgtcgtg agccgtagcg gcgccgcgta gacttggtag
      781 agcgccccgt cggcgaagtc gtacgtcatg atcgcgttga agtagccttc gcgatcgggg
      841 ccgagcgcgg cttttcggtt ggcgtcagcg atcacgtcgg agggtgactg cttctctggg
      901 cgcttcacgt catggacggg aattcgcctg agctgccccg gcatcggcat gggctgcggg
      961 acttcgacga cgacgggctg ccgtggggga tccttctccg gggtggcgcg cacgtactgc
     1021 ggcggtggtt gacgcgaggc gcacccgatc gtggccgcga cgaacaggat ggagcttctg
     1081 ttgtgcatcc gactctccag cgttgggggt gaggggaaag agtccgggcg tcagccctgg
     1141 accttcgacc agtggaactc gtcgatgaag aggccaatcg ggttcgtggc gagctgctcg
     1201 tcggtctcag ggacgcgaac gagcacacgg aacacgccac gccagatggt gctcgtggtc
     1261 tcctcgccgc ctttgtccca gcccttctcg acccaatcga cctgccacgt gtcggcagag
     1321 agctggacga gggacttgac gtcgaccgtg acccgctcgt cctcgccgcg cttgatcggg
     1381 ttggtctcct gcgcgaacgc cgtgaggtgg ttcgcggcgc tgccggtgat gagcgtgtaa
     1441 gcgtctgtcc agtaccgctt catcacgacg acgtcggacg acacggtccg cgtgtcggtg
     1501 atgaagcgac ggaggtggta cttcaggacc gcgtccgtcg gtttccactc ccgcgcggat
     1561 tggccgactg ggcccagata ggttggcgca ccgatccgat cgatctggac aatgtgagga
     1621 accgccttcg gctgaacgcc gaggtacacg gtggcgagga gcgccacgaa caccagaccg
     1681 agcgccagca gggcggcgag ccgccagttg taggcctgcg ccagcgccgt ccccatcctt
     1741 ctgtcccatt cctggcgagc ccggcggtag cgagtctcga cctcaccctc gggcttccac
     1801 cgcttgttcg cgatcgggtc catggtcctc caggattgcg agtcacaggc gtcgactgag
     1861 tcgcgcagcg cgagcggctt ctatccggcg ccctgctcgc ttgattctcg tgacccgcac
     1921 cggcttcgtt gccgttgaga ggttcacggc cgctcgcggt ggagaagtgc cgcgcgccga
     1981 ccttcttaag agcgcccgcg ccagtccggg tacatcaggc gtcgcccgat ctcggcatgg
     2041 cactcggcgc atgtgacgtc ccggcgtcgt cgcgaccagc cgccgactcc tggggcgagt
     2101 ccacagaggg agcgtgccga ttcagatggc cgcgcgtgga cgcgtgcgtg tcctgccggc
     2161 ggcggcggtt cacgcgaagg acccacgacg tgccatgctg aacaaatgat caggtatcgt
     2221 caagacccgc ggtgacgctg gcgtgttagg tacgcacgtg accgtgactt cccgagccat
     2281 ccaggcggcg gcgatgtggt gtgtggtgac tgccggtttt tctagcaatg cggccgacgc
     2341 accgagagtc gagaagcgat gcgggtgggt gtcgaacccc acaccaggta actggagcct
     2401 gatcgacaag gacggcgagt gggcgatcgc agcccaggga gaagaaggag ctctggacgg
     2461 actgccggag gatcgacctt caggcaggca ctggtgggtg agcaccaaca gtggcggcta
     2521 cggctacggg tgcatgtctc tgcaggttgc agtcgacaag cagacgaagc gggtcctcaa
     2581 gatcgagggt ggaaagtcac tgcccctgac gacctgcagg aaggaccggg caatccggaa
     2641 gctcgagccg cgctgatcgt tctcacgagt cagtttcgtg ggggacgctg ctcctgatca
     2701 cgtcttgggc ggctcgtgtc ggtgcacgtt ggccgatcgc tccgcaccgg cgcggaagct
     2761 gtcacggaac ggggctgatg cccgtgacag gcgggccttg aatccttcca cggcggagcg
     2821 ccctgcgctc gcagcggcgc ttgttcctgc gcctagcgca gtgtcgatgg cgcctccccc
     2881 ttgttgggtc cccttgctcc cacctccgaa caggacaccc gcgccgtacg cggcgcgctg
     2941 gccgagcgca gaggcgccgc tgaccgcagc ccgagtcgct gcgaccattc cgcctgcgac
     3001 gcctgccgcg agcaggctgc ctgaggtgac gttctgagcg acaccagccg cgctcagcga
     3061 cggcgagccc gccaggagac cggccgccaa gctcggaaca tgccaggtga ggaacgcaat
     3121 cgccgcagcg gtgaggagga cgctccacag ctcgttgagc ttgatctcgg gtccagagaa
     3181 gtggatggac gcaagcacgg gatcgacgac tgcgatgatg aaagccagca ccatcagctt
     3241 gatgccggcg ctcaccacag caccgatcgc cttctcggcc aggaacttcg tctgcggcaa
     3301 cagcgcccag gggatcagga ttccggcgac agccaggatg acgtagtact cgaggacagc
     3361 aaggaacgcc tggatggcca tgatcaggaa cgcggccatg atcagcaaat agctgatccc
     3421 gaagatgatg acatccccga ggtccgtgac gctgatgtcg tcgagcgcct tcgcgagctg
     3481 ttcggtcgcc gtgaggccgt atgcggcaat ccgggaggga tcgagcagca ggttgacgcc
     3541 cccgggatgt cctccggccg tgagcccggc gttgatgagt gagtcgacga aggccttggc
     3601 gttgctctgg aacgacttcg tgaaccagag ccagaacccg aggaacagga tcttcttgat
     3661 cacttcggtc aggcgctcgc ccccgcccag ggcccaccag aagcccacca ggactatgtc
     3721 gattccagcc aggatggcca agaggccgtt gatcgtcggg ccgagccgcg agtagcccgc
     3781 ctggaagacg ccgacgaaag tggtcagcgt cgtcgtgaga gtgttgaact ccacgggtgc
     3841 gcctcagggg agactgttca ggacgcgggg tgtctgtccc cggctggtgt aactgtcgcg
     3901 gcgccggagt ttggcctctc gcacgaggag cttctcgccc gccgacgaa
//
LOCUS       DASWVF010000866         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_203394, whole genome shotgun sequence.
ACCESSION   DASWVF010000866 DASWVF010000000
VERSION     DASWVF010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203394"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGD55_15315"
     CDS             <1..813
                     /locus_tag="VGD55_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY0871768.1"
                     /translation="VEPTACTPGSGWEKGQVENQVGTSRERLFTPRLRFAGYAELNGW
                     LEARCRSLARESGHPEVKERMISEVFEDERAALIPYAGPFDGFHEKTLGVQKNCLVQF
                     DRNRYSVEARAVGRAVQLHAYAERIVVRLDGEIVAEHPRRFGRDQVVYDPWHYVPVLA
                     RKPGALRNGAPFRDWRLPASLAQVRRCLSSHADGDRQFVGILGAVLTDGLDVVEAACA
                     EALAAKLFSRDVVLNLLARRREPTPPANVASIGPALTIEPVADCARYDTLRR"
     gene            850..1551
                     /gene="istB"
                     /locus_tag="VGD55_15320"
     CDS             850..1551
                     /gene="istB"
                     /locus_tag="VGD55_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY0871769.1"
                     /translation="MGQLKLAGMRAAYDEILEEGIRRQQPMPHVIGALLRAQTSAAQA
                     RSIAYQLGMAKFPMAKTLSQFDFNASPVNEGLVRERHGGDFLDQQRNLVVVGGTGTGK
                     THIAVAIGSNCVRQGRRARFYNTVDLVNRLDAESRAGKAGQLASSLIQVDLVILDELG
                     YLPFPQSGGQMLFHLLSKLYERTSVIVTTNLAFAEWPSVFSDAKMTTALLDRLTHHCD
                     IIETGNESWRIKNRG"
BASE COUNT          268 a          521 c          569 g          300 t
ORIGIN      
        1 gtcgagccga cggcgtgcac gccgggctca ggctgggaga agggacaagt cgagaaccag
       61 gtcggcacgt cgcgtgagcg gctgttcacg ccgcggctgc gctttgccgg gtatgccgag
      121 ctgaatggct ggctcgaggc acgctgccgg tcgctggcgc gggagagtgg gcatccggag
      181 gtgaaggagc ggatgatctc ggaggtgttc gaggatgagc gggcggccct gatcccgtac
      241 gctggtccgt tcgacggctt tcacgagaag acgctgggcg tgcagaagaa ctgtctcgtt
      301 cagtttgacc gcaaccgcta cagcgttgag gcccgcgcgg tcggccgggc ggttcagctg
      361 catgcttatg ccgagcggat cgttgttcgg ctcgacggcg agatcgtcgc cgagcacccg
      421 cgtcggttcg gccgtgacca ggtcgtctac gacccgtggc actacgtgcc ggttctggcg
      481 cgcaagccgg gcgcactgcg caacggtgcg ccgttccgcg actggcggtt gccggcgtcc
      541 ttggcgcagg tgcgccgttg cctgtcctcc cacgccgatg gcgatcgcca gttcgtcggc
      601 attctcggtg ccgtgctgac cgacggcctg gacgtcgtcg aggcggcctg tgctgaggcg
      661 ctggcggcga agctgttcag tcgcgacgtg gtgctcaatc tgctggcacg ccgccgtgag
      721 ccgacaccac ctgcgaacgt ggcgtcgatc gggccggcgt tgacgatcga gccggtcgcc
      781 gactgcgccc gctacgacac gctcagacga taggagagtg cggtggaacg cgttgagatc
      841 ctggatttga tgggccagct gaagcttgcc ggcatgcgtg cggcctatga cgagatcctc
      901 gaggagggga tccgccgaca gcagccgatg ccgcatgtca tcggcgctct gctgcgcgcc
      961 caaacgtcgg cggcgcaggc gcgctcgatc gcctaccaac tgggcatggc caagttcccg
     1021 atggctaaga cgctgtcgca gttcgacttc aatgcgtcgc cggtcaacga gggcctggtg
     1081 cgtgagcgtc atggcggcga cttcctggat cagcagcgca acctggtcgt ggtcggcggc
     1141 accggcacgg gcaagacgca catcgcagtg gcgatcggct ccaactgtgt gcgtcagggg
     1201 cggcgtgctc gcttctacaa caccgtcgat ctggtcaatc ggctcgatgc cgagtcacgc
     1261 gctggcaagg ccgggcagtt ggcatccagc ctcatccagg tcgacctcgt gatcctggac
     1321 gaactcggct acctgccgtt cccgcaatca ggcggtcaga tgctgttcca tctgttgtcg
     1381 aagctctacg agcgcacgtc ggtgatcgtg acgaccaacc tcgcctttgc cgaatggccc
     1441 agcgtgttca gcgacgccaa gatgaccacc gcgctgctcg atcgcctcac gcatcactgc
     1501 gacatcatcg aaaccggcaa cgaaagctgg cgcatcaaga accgcggctg accgccctca
     1561 tgcaaccgct ggtacgggtc ccgtttgcac gctgacggtg ggtcccaatt ggacgctgat
     1621 tgacacacga cggtcttggc gggcaatccg atcttgtc
//
LOCUS       DASWVF010000867         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_432607, whole genome shotgun sequence.
ACCESSION   DASWVF010000867 DASWVF010000000
VERSION     DASWVF010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432607"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VGD55_15325"
     CDS             <1..1418
                     /locus_tag="VGD55_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871770.1"
                     /translation="HATAAEGCQQVPIAATVGGDPVGVEFDASTRSVFVLNQGDDSVS
                     VVNGATCNAAVTTGCGTQAPQIATGADPAAFDVDAASNTIYVSNQDDNDVSVLTIDTC
                     APDHTTGCRQLPASTSIGAEPVGAALDSATNTIYVANQDGADLSVINPAACNSRVSTG
                     CATRWTTATTGDNPLGVAVNQTTDTIYTTNSSPENNPDGSAQSLDGSTVSVIDGAHCN
                     ASNDSSCNSAATITVGQGPAALAIDEATNTIYVTNFDDGTVSVIDGATCDALVKTGCG
                     ANPPTILVGHNPEGVAFDSATGTLYVSDTGDNTVSVINAKTCDAGVVTSCTKPAPTIS
                     VGKEPRGMAIDTATDTVYVANYADRTVSVINGGTCNASVTSGCGQQPPTMTTGSSPKR
                     GVAVDQATGEVFVDSYYADAVDEFNGSTCNSTVSSGCDQQPVVVPAGGQPVSVVVDPA
                     TDTVYAADDADGLLSFFAPPR"
BASE COUNT          323 a          526 c          478 g          247 t
ORIGIN      
        1 gccatgcgac tgcggccgag ggctgccagc aggtgccaat cgccgcgacc gtaggtggcg
       61 accccgttgg agtcgagttc gacgcgtcaa ctcgcagcgt cttcgtgttg aaccagggcg
      121 atgacagtgt gtcggtcgtc aacggcgcaa cctgcaacgc cgcagtgacg accggctgcg
      181 ggacgcaggc accgcagatc gcgaccggcg cagacccagc cgctttcgac gtcgacgctg
      241 cgtccaacac gatctatgtg tccaaccaag atgacaacga cgtatcggtg ctcaccatcg
      301 acacttgcgc accagaccac acgacggggt gcaggcagct tcccgcatca acgtcaatcg
      361 gggccgaacc ggtcggagcc gcactcgact ccgcgaccaa cacgatatat gtggccaacc
      421 aggacggcgc tgacctgtca gtgatcaacc cggcggcgtg caactcccgc gttagtaccg
      481 gctgcgccac gaggtggact acggcgacga cgggtgacaa tccacttggc gtcgcggtga
      541 atcaaacgac cgacacgatc tacaccacca actcgagccc ggaaaacaac cctgacggtt
      601 cagctcagtc gctcgatggc tcgaccgtgt cagtgatcga cggagcgcat tgcaatgcga
      661 gcaacgactc gagctgcaac tcggcggcta ccatcaccgt gggtcaaggc ccggccgcgc
      721 tcgcgatcga cgaggctacc aacacgatct atgtcaccaa cttcgacgac ggcactgttt
      781 ccgtcatcga tggcgcaacg tgcgacgcgc tggtgaagac cggctgcggt gcgaacccgc
      841 cgacaatcct ggtcggtcac aatccggaag gcgtcgcctt cgactctgcc acgggcaccc
      901 tctacgtctc tgataccggc gacaacaccg tgtcggttat caatgcgaag acatgcgacg
      961 caggcgtggt gacgagctgc accaagccag cgccaaccat ctcggtcggc aaggagccac
     1021 ggggcatggc catcgacacc gctacagaca ccgtctatgt cgcgaactac gccgaccgca
     1081 cggtgtctgt gatcaatggc gggacgtgca acgcaagcgt gacatccgga tgtggccagc
     1141 agcctccgac aatgaccacg ggcagttcgc cgaagcgcgg cgttgccgtc gaccaagcga
     1201 ccggcgaggt tttcgtcgac tcctactacg ccgacgccgt cgatgaattc aacggttcga
     1261 cctgcaactc cacggtgagc tccggctgcg atcagcaacc ggtagtagtt ccggcgggag
     1321 ggcagcctgt gtcggtagtc gtcgacccgg ccacggacac ggtctatgca gccgacgacg
     1381 ccgacggcct gctctcgttc tttgcacccc cgcggtgaca ggatcagtcc gaccacctca
     1441 agggtaggca ccgcagtgag ccgaaccagc aggatcttcc cgacagtggc gggactcggc
     1501 gccgcgctga ttgtcctggc tgcgtgcgga tcacccggcc ggatcgacaa ggccggtgcg
     1561 atcgaacacg ccgt
//
LOCUS       DASWVF010000868         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_407319, whole genome shotgun sequence.
ACCESSION   DASWVF010000868 DASWVF010000000
VERSION     DASWVF010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407319"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1578
                     /locus_tag="VGD55_15330"
     CDS             79..1578
                     /locus_tag="VGD55_15330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0871771.1"
                     /translation="MSEPRRITHWIGGKPYTGESARHGDVFDPATGQVTGHVDFASSD
                     DVGLAVSAAVDASHSWRSASLSKRTSVMFAMRELLHERADDVAALITAEHGKVLSDAA
                     GEVARGLEVVEFACGIPQLLKGGFSENVSTKVDVYSIRQPVGVVGVITPFNFPAMVPL
                     WFVPIAIACGNVVVLKPSEKDPSAANLLAELFADAGLPDGVLNVVHGDKVAVDALLNH
                     PAIGSISFVGSTPIARYVYETGTKNGKRVQALGGAKNHMVVLPDADLDLAADAAVNAG
                     FGSAGERCMAISAVVAVDPVGDELVERIRTRMATLKIGDGRRGCDMGPLVTRPHRDKV
                     ASYLDAGVSAGATLVVDGRGVAIDGDPDGFWLGPSLLDHVTPPMSCYTDEIFGPVLSV
                     LRSPTYDAALALVNASPCGNGTAIFTNDGGAARRFQNEVTVGMVGINVPIPVPMAYYS
                     FGGWKSSLFGDTHAHGLEGVHFFTRGKVVTSRWLDPSHGGLNLGFPTNG"
     gene            1743..3629
                     /locus_tag="VGD55_15335"
     CDS             1743..3629
                     /locus_tag="VGD55_15335"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY0871772.1"
                     /translation="MRTITARVADAPSGPAVPWRAAAFARLTAAARAIRFAPTPAIAT
                     PRAMAKTAGLLYICASTMSLVALGLHQLPQVHRGPLVVIAAGSLAIGITVVLVGQLWP
                     PWCYHLLILNANIAIAVGMYEGGGGRTSLAYATLYTFVAINCFFFFAWPSACLHLAVA
                     IGISVFTLSILGEADFGQVLLLPGTLIVVAVVVGRLVRAADAAELDTLTGLPNRRGLD
                     RHMSEAVADAALGTHTVALAFLDVDHFRSVNHSEGQSGGDRLLRSIAAAWRPLLRDGD
                     FLARNGSDEFALLLIDAQPGDVTNVTGRLRAAMPRAHTCSAGVADWQPGDTVSLLAAR
                     ADTALYESKRGGRNRVTYHDSTHNTEDAVMRKAVAEGEVIAYYQPIIELGSGLVTGFE
                     ALARWIRPGHGMVAPDAFIPQAEQSGAIEDIGAMMLRDACGQVAIWSKAHGRPLVASV
                     NVCGRELRNPTYVRRVRAILADAELPASQLVLELTETAFEGDADPVVEALAELHALGI
                     RIAIDDFGTGYSSLSRLDQLPVDVVKIDRSFVSPLPPEAIGAPLVAAIAAMAHALGLQ
                     VVAEGIEHPSQAALLFSLGCDKGQGFWFARPCPADQVDLSRREPARSLPGTADAAATV
                     RKVG"
BASE COUNT          602 a         1228 c         1236 g          675 t
ORIGIN      
        1 gctgaccgag gcagaggcga ccgagggcct cgacattctc acgaaggcga tcgccgcgct
       61 ggagggagca gccacatcat gagtgagccc cgtcggatca cccattggat cggtggcaag
      121 ccgtacacag gggagtcggc ccgccacgga gacgtcttcg accccgcaac aggtcaggtg
      181 accggtcacg tcgacttcgc gtcgtccgac gatgttggtc ttgctgtgag cgccgccgtc
      241 gatgcgtcgc acagctggcg ctcggcatcg ctgtcgaagc gcacctctgt catgttcgcg
      301 atgcgcgagt tgctgcacga acgggccgac gacgtcgccg cgctcatcac cgcagaacac
      361 ggcaaggtac tgagcgatgc agcgggtgag gtcgctcgcg gactcgaggt tgtcgaattc
      421 gcctgcggga tcccccaatt actgaagggc ggcttctccg agaacgtctc gacgaaggtt
      481 gatgtctact cgattcgcca accggtcggc gtcgtcggcg tgatcacgcc gttcaacttc
      541 cctgccatgg ttccgctctg gttcgtgccc atcgcgatcg cgtgcggaaa tgtagttgta
      601 ctgaagccaa gtgaaaagga cccgtccgcg gccaatctgc tcgccgaact cttcgccgac
      661 gcgggcttgc cagacggcgt cctcaatgtc gttcacggcg acaaggttgc cgtcgacgcg
      721 ctactgaacc accccgccat cggctcgatc tcgttcgtcg gctcgacgcc gatcgcacgc
      781 tacgtctacg aaaccggaac taagaacggc aagcgggtgc aggcactggg tggcgcgaag
      841 aaccacatgg tcgtgctgcc cgacgccgac ctcgatcttg ccgccgatgc agcggtcaac
      901 gcagggttcg ggtccgccgg cgaacgatgc atggcgatct ccgcggtggt tgcagtcgac
      961 ccggtgggcg acgaactggt cgagagaatc cgcacccgca tggccacgct gaagatcggc
     1021 gatggccgcc gcgggtgcga catgggaccg ctcgtgacgc gcccgcatcg cgacaaggtc
     1081 gcgtcgtatc tcgacgccgg cgtttcggcg ggcgcgacct tggtggtcga tggccgcggc
     1141 gtggctatcg acggcgaccc cgacggcttc tggctcggac cttcgctgct cgaccacgtg
     1201 acgccgccga tgtcgtgcta caccgacgaa atcttcggcc cggtgctgtc ggtgttgcgg
     1261 tcgcctacgt acgacgcggc actggcgctg gtcaacgcga gcccgtgcgg caacggcact
     1321 gcgatcttca ccaacgacgg cggcgccgcg cgtaggttcc agaacgaggt caccgtcgga
     1381 atggtcggca tcaacgtgcc gatcccggtg ccgatggcgt actactcctt cggtgggtgg
     1441 aaatcgtcgc tgttcggaga cacgcacgcg cacggtctcg agggcgtgca tttcttcacg
     1501 cggggcaagg tcgtcacctc gcggtggctc gatcccagcc acggcggctt gaacctcggc
     1561 ttcccgacga acggctgacc gagcggcggc gtcacccgtt tggagtagcg ccggcaggcg
     1621 tgcgtcgcgt tcctgctcat gcatccccga ctgcggccga taggcgtgcc acggtgcccg
     1681 gacggctgaa cgcgccatgg ttcatcaccg gggcgacgca agcatcgacg ggattggtgt
     1741 aggtgcgcac gatcacggca cgagtcgcgg acgctccgag cggccctgcc gtgccatggc
     1801 gagccgcggc cttcgcgcgg ctgaccgccg ccgctcgggc aatccgcttt gctcctacgc
     1861 cagcgattgc gacgccccgg gcgatggcga agacggccgg actgctctat atctgcgcat
     1921 cgacgatgtc attggtcgcg ctaggtctgc accaactccc gcaagtacat cgcggcccgc
     1981 tggtcgtcat cgcagccgga tcacttgcca tcgggatcac ggtggttctc gtcgggcagc
     2041 tctggcctcc ttggtgctat cacctgctga tcctgaacgc caacatcgcg atcgccgtcg
     2101 gcatgtacga gggtggtggc ggccgtacat cccttgctta cgcgacgctc tacacgttcg
     2161 tcgccatcaa ctgcttcttc ttctttgcgt ggccgtcggc atgccttcac ctcgcggtcg
     2221 ccatcggcat cagcgtgttc acgctgtcga tcctcggcga agccgacttc ggtcaagtac
     2281 ttctcctgcc cggcaccctg atcgtggtcg ctgttgtcgt cggccggtta gtaagggctg
     2341 ccgacgcagc cgaactcgac accttgaccg gattgccgaa ccgacgcggc ctcgatcggc
     2401 atatgagcga ggctgtcgcc gacgcggcgc tcggcacgca tacggttgct ctcgctttct
     2461 tggacgtcga tcacttccgg tcggtgaatc acagcgaagg tcaaagtggt ggggaccggc
     2521 tcctgcggtc gatcgctgcc gcttggcggc ccttgcttcg tgatggcgac tttctcgcgc
     2581 gcaacgggag cgacgagttc gcgttgctcc tcattgacgc tcagccgggc gacgtcacga
     2641 acgtgacggg tcgactgcgc gccgcgatgc cgcgagcgca cacatgttcg gcaggcgttg
     2701 ccgactggca gcccggcgat acggtgtcgc ttctcgcagc ccgcgctgac acggcgctgt
     2761 acgaatcgaa gcgcggcggg cgcaacaggg tcacctacca tgacagcacg cacaacacag
     2821 aagacgcagt catgcgcaaa gccgtcgccg agggcgaggt gatcgcgtac taccagccga
     2881 tcatcgaact cggcagcggg ctcgtgaccg gcttcgaggc gctggcgcgt tggatccggc
     2941 ctggccacgg catggtcgca cccgacgcgt tcatcccgca ggccgagcag agcggcgcca
     3001 ttgaggacat cggcgccatg atgctgcgtg acgcctgcgg gcaggtggcc atctggtcga
     3061 aggcgcacgg ccgcccgctt gttgcgagcg tcaacgtctg cggccgcgaa ttgcgcaatc
     3121 ccacctacgt gcggagggtc agagcaattc tggcggacgc cgagctgccg gcgtcccagc
     3181 tcgtactcga attgaccgag acggccttcg aaggcgacgc ggatccggtt gtcgaggcgc
     3241 tcgcggagtt gcacgctttg ggcatcagga tcgcgatcga cgacttcggc acggggtatt
     3301 caagcctgag ccggcttgac cagttgccgg tcgacgtggt caagatcgac cgctccttcg
     3361 tcagcccgct gccccctgaa gcgatcggcg caccgcttgt cgcggccatt gcggccatgg
     3421 cacatgcgct cgggctgcag gtggtggccg agggcattga gcatccttct caggctgcac
     3481 tcctgttcag cctcgggtgc gacaagggtc aaggtttctg gttcgcgcgg ccatgtccgg
     3541 ccgaccaggt cgacctgtcg cggcgagagc ccgcgcgatc actgccgggg actgccgacg
     3601 cggcggccac cgtgcgcaag gtgggctagc aagcccgaaa ttccatcggc gctaccggct
     3661 cggcgtcgat gcttttgggc attgagttgg caccatagag gccgcacgcg accgccgtgc
     3721 ggaactgagg ggtgcccgat g
//
LOCUS       DASWVF010000869         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_356365, whole genome shotgun sequence.
ACCESSION   DASWVF010000869 DASWVF010000000
VERSION     DASWVF010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356365"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VGD55_15340"
     CDS             <1..1320
                     /locus_tag="VGD55_15340"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0871773.1"
                     /translation="GLARDFAICHRWFAPHPGPTFPNRYYELTGRPNIDAWGAWEYAN
                     SSPLRPVFTDTIFDHLSARGVSWKYFEHAYSFMRFFERHTYDSQNLVDFDDPKIGFNA
                     LAKSGNLPSVSFIDPHYVDYPPGSFCDEPPSDIRNSQKFIRHLVETVVSSPKWEKTLL
                     LITYDEHGGFYDHVPPPTAAKVSPEMLPTTGLRVPAIVISPWVKGSTVFGTDKLHFDH
                     TSILKTIARRFMSSDPPYMGARYAAAHDLSEILDNELRPEQFLPFIPYTLVYEASGMC
                     LDVQGASTAAGASLCQSLPVAYDPDGPGMQNFRFEDAGGGFVYIRTFAGFYVTANAPA
                     NHHGETNNTVGITQELRYASSAGHKNVDRQRWKLTSSAVAAINQTSYTISCAEFPGLV
                     LQPADGSTPSGVAVVLGHPTPSHSPMMVPNPWVVTSPLLPPPGLVNA"
     gene            1381..>2310
                     /locus_tag="VGD55_15345"
     CDS             1381..>2310
                     /locus_tag="VGD55_15345"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEY0871774.1"
                     /translation="MSFTLDELAYSTELVGRWVPPTPQYAWPLLGAAVGAQVWVKHEN
                     HTPTGAFKVRGGLVYADRLRSRRPHVAGIVSATRGNHGQSLAFAGRARDVPVTIVVPF
                     GNSPDKNAAMRGFGAEVIEHGHDYQAAREYSVALGAERGLETVPPFHDDLVLGVATYA
                     RELFAAAPFDAVYVGVGMGTGISGLIRVRDLLGLPTEIIGVVSELAPATALSFTAGSV
                     VTTETANTFVDGVACRVPEAAAISAIVAGAARVIQVSEDASADAVRLLLSTTHNIAEP
                     AGAIALAGLLSERELMQGRRVAVIQSGGNIDSPM"
BASE COUNT          418 a          738 c          714 g          440 t
ORIGIN      
        1 gggctcgctc gcgacttcgc catttgccat cgctggttcg ctccgcaccc ggggccgacc
       61 ttcccgaacc gctactacga actcaccgga aggccgaaca ttgacgcatg gggcgcttgg
      121 gagtacgcga attccagtcc cttgcggccc gttttcaccg acacgatctt cgaccatttg
      181 tcggcgcgtg gagtctcgtg gaagtacttc gagcacgcct acagcttcat gcggttcttc
      241 gaacgtcaca cctatgactc gcagaacctc gtagatttcg atgatccgaa gatcggcttc
      301 aacgctcttg caaaatccgg aaacctgccg tcggtgtcgt tcatcgatcc gcactacgtc
      361 gactaccctc ccggcagctt ctgcgacgaa ccgccatcag acatcaggaa cagtcagaag
      421 ttcatcaggc acctcgtgga gacggtcgtc agcagtccca aatgggagaa gacgctgttg
      481 ctcatcacct acgacgagca cggtggcttc tacgatcacg tgccgccacc caccgcggcg
      541 aaagtgtcgc cggagatgct cccaacaacc gggctgcgag ttccggccat cgtcatctca
      601 ccgtgggtga agggcagcac ggtatttggt acggacaagc tgcatttcga ccacacctcc
      661 attttgaaga ccatcgcgcg ccggttcatg agcagtgatc ctccgtacat gggtgctagg
      721 tatgccgcag cccacgacct ctctgagatt ctcgacaacg agctccgacc cgaacagttc
      781 ctgccgttca ttccgtacac actggtctat gaagcctccg ggatgtgcct cgacgtgcaa
      841 ggcgcaagta cagcggccgg ggcgtcgctt tgtcagtcgt tgccggtcgc atacgacccg
      901 gacgggcctg gcatgcagaa cttccggttt gaagacgcag gcggcggctt cgtctacatt
      961 cgcacgttcg cggggtttta cgtgactgca aacgcgcccg cgaaccacca cggcgagacc
     1021 aacaacacgg tgggcatcac gcaggagttg aggtacgcga gttcggcagg tcacaagaac
     1081 gtggatcggc agcgatggaa gctcacgtcc tctgccgtcg ccgcgatcaa ccaaaccagc
     1141 tacacgatct cgtgcgcgga gttcccagga ctagtgctgc agccggccga cggcagtacc
     1201 ccttcaggag tcgccgtcgt gctcggtcat ccgacaccgt ctcactcacc gatgatggtg
     1261 ccgaatccat gggttgtgac ctcgcctctc ttgccgcccc cgggactggt caacgcgtag
     1321 gggtcgagca tctcgtccgg cccggggcat cgggggcagc gcaggtgcga gactacgcac
     1381 atgtcgttca ctctcgatga gttggcctac tcgaccgagt tggtcggtcg atgggtgccg
     1441 ccgactccgc aatatgcatg gccactgctc ggtgcagcgg tcggcgcgca ggtgtgggtg
     1501 aagcacgaga accacacgcc gaccggagcg ttcaaggtgc gcggcggcct tgtctacgcc
     1561 gaccggctgc gatcgcggcg ccctcacgta gccggcatcg tgtcggcgac gcgaggcaat
     1621 catgggcaga gcctcgcgtt tgccgggcgg gcgcgcgatg tgccggtcac gatcgtggtg
     1681 cccttcggca acagtcccga caagaacgct gccatgcgcg gctttggcgc cgaggtcatc
     1741 gaacacggtc acgactatca ggccgcgcgc gaatattcgg tcgcactcgg tgccgagcga
     1801 ggcctcgaaa ccgtgccacc ctttcacgac gatctggtgt tgggcgtggc gacctatgcg
     1861 cgcgaactct ttgcggccgc tcccttcgac gccgtgtatg tgggcgtcgg catgggcacc
     1921 ggcatctccg gactgattcg ggttcgcgac ctgctcggcc tgccgaccga gattatcgga
     1981 gtggtgtcag agctcgcacc cgcgaccgcg ctgtcgttca cggccggatc ggtggtcacg
     2041 accgaaactg cgaatacttt cgtggacggc gtggcgtgcc gagtccctga agcggccgcc
     2101 atttcggcga tcgttgcagg agccgctcgc gtgatacagg tcagcgagga cgcctcggct
     2161 gacgccgtgc ggttgttgct ctcgaccacg cacaacatcg ccgagccggc cggcgcgatt
     2221 gcgttggcgg gcctgctttc ggagcgcgag ttgatgcaag gtcgtcgagt ggcggtaatt
     2281 caaagcggcg ggaacatcga cagcccgatg
//
LOCUS       DASWVF010000870         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_305015, whole genome shotgun sequence.
ACCESSION   DASWVF010000870 DASWVF010000000
VERSION     DASWVF010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305015"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(865..>1681)
                     /locus_tag="VGD55_15350"
     CDS             complement(865..>1681)
                     /locus_tag="VGD55_15350"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0871775.1"
                     /translation="TQGSPGEVNIDGVQAFPAAKNTVGKTIADQLDAQRHSWKSYQED
                     LPTSGADLVNFSDGVFTNNTDFSAIMPILNPPLAGNGIVALYAAKHNPFVYFKSIQDG
                     HDRDNSLGNIFSFDGPDGLFADLGAGKAPTLSFIAPNQCNDQHGRGNAGPFCNFDPND
                     DGSQSGLNPALIYLGDVTVQRLVNAIHGSKVWREGNSAIVVVWDENDYSLSPNNNQVM
                     VIVDTNYGRHGISSGRRYTHFSLLKSLEAGLELPCLNHACDHEVAVMSDLFSR"
BASE COUNT          313 a          521 c          530 g          317 t
ORIGIN      
        1 cactatcatg tcgtaactga cgcggtagaa ccttactcat aacacgcccg gccatcaccg
       61 cagcgaaact tccggccgct accccgacga acgcaatcac gacaaagccg gaaagggcga
      121 tcgttcgggc ggtgattgcc acgcccggcg gacgatcgtc gcaacggctc cggatcacaa
      181 gctcctttta gtccgaacca gcctccggtg tcatttgcac gcggtgagag cacgcggcgc
      241 gccatgacga tcgggtaaaa tgcacgatgg tggcgtcgct gccttccgcg gccttgtccc
      301 gttcaaacgc cgattgcggc gttacggcct caccaccccg gacagttgtt tgatccctag
      361 tcgacgcgtt ccaacgcgcg cgacgatcgc ctcgcctata gccgttcgcc cagggatttc
      421 acgtatcggt cgcagaggta cagccgttgc aatattctcg cgtcgccttg gcgactcaac
      481 tggagagcga cagatgacca gccggcattg ggcgtggacg gcgagcgtgc tcgggggtcc
      541 cctgctggat gacagcctgt ggcaatggac ttcatcggcg accgaccggg taaacgatca
      601 tgtctggtcc cggcgaggaa ggcagggacg gtccagatcg tcgatgcggg caggcgctga
      661 acgtgatcgg agggttccga aggcgccgac tcagcggccg agccgccccc aacggcgccg
      721 agttccgtag ccgtccgagg ggggaccgtg tgggtcgcaa accgaggcga cccacaccct
      781 tgaagctcac cgtcaacccg accagtcata catgtttctg acggcgcacc gacaagaccc
      841 gtgcccgacg gcccgccgac tctgtcagcg gctgaagagg tccgacatca cggcgacttc
      901 gtggtcgcag gcatggttca agcagggcag ctcgagcccc gcttccagcg acttgagcag
      961 ggagaagtgc gtgtagcgcc gcccgctcga gatgccgtga cgaccgtagt tcgtgtcgac
     1021 gatgaccatg acctggttgt tgttcggaga caggctgtag tcgttctcgt cccacacgac
     1081 gacgatcgca ctgttccctt ccctccacac ctttgagccg tggatggcgt tcaccagcct
     1141 ctggacggtg acgtctccca agtagatcaa agcggggttg aggcccgact gcgagccgtc
     1201 gtcgttaggg tcgaagttgc agaaaggccc ggcgtttccg cgcccatgct gatcgttgca
     1261 ctgattcggg gcgatgaacg agagcgtcgg agccttgcca gcgccgaggt cggcgaacag
     1321 gccgtccgga ccatcgaaag agaagatgtt cccgaggcta ttgtcccggt cgtggccgtc
     1381 ctgaatgctc ttgaagtaga cgaacggatt gtgtttggcg gcgtagagcg cgacgatccc
     1441 gttcccggcg agcggcgggt tgagtatcgg catgatcgcg ctgaagtcag tgttattcgt
     1501 gaagacaccg tcgctgaaat tgacgaggtc cgcgccgctg gtaggcaggt cctcctggta
     1561 actcttccaa ctgtggcgtt gcgcgtcgag ctgatcggca atagtcttgc cgaccgtatt
     1621 cttcgcggcc ggaaacgcct ggaccccgtc gatgttgacc tcgcccggcg agccctgcgt
     1681 t
//
LOCUS       DASWVF010000871         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_75267, whole genome shotgun sequence.
ACCESSION   DASWVF010000871 DASWVF010000000
VERSION     DASWVF010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75267"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGD55_15355"
     CDS             complement(<1..548)
                     /locus_tag="VGD55_15355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0871776.1"
                     /translation="MTSSPGITTAAARLRPLRIALVLQGIAPWVPVEKIFMTQLGFTP
                     TLFALMAAAYGATVPLLEIPAGILADRWSRRGVLMLASLASLASVIVGGLSHNVATYL
                     ASAMLLGVYFAMQSGTVDAIVYDTLIEEAGDADGFEKQYGRIQALNSVALTASALAGG
                     VIAALVSARFTYLLTIPTSVAGL"
     gene            complement(578..1591)
                     /locus_tag="VGD55_15360"
     CDS             complement(578..1591)
                     /locus_tag="VGD55_15360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEY0871777.1"
                     /translation="MPMTDDATLVGDDAAFMQVADRYRSELRVHCYRMLGSFTDAEDH
                     VQEALLRAWRNRASFEGRSPMRAWLYRIATNVCLDTLRTHPERVVLVGGGGDPAPSEI
                     PWLQPYPDLLLDRQASRADEPDALVVARETIELAYLTAIQLLSPNQRAVLILRDVLDF
                     SAAETADLLDTSVASVTSALQRARATLKQHRPDEPTTWTPTAEPSVEERALLQRYMDA
                     HARADSDAVVAMMREDVRFTMPPEPTLYEGKAALAKFFAETFTPGAVGEFRLVATRAN
                     RHPAAANYVRAPGDTVFRAMSLDVLRFEAGQLAEITTFDTDLFPLFGLPEILPETDLL
                     ASD"
     gene            complement(1625..2644)
                     /locus_tag="VGD55_15365"
     CDS             complement(1625..2644)
                     /locus_tag="VGD55_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0871778.1"
                     /translation="MRAFVVTGPHQGAVQEVEAPVAGVGEVVVDVHRVGVCGTDAEFF
                     TGVMAYLHQGHAAYPLRLGHEWCGKVAAIGDAVPAEWLGRRVTGDTMLGCGHCHRCLN
                     GRQHVCVDRYEIGIRRGWPGALAEQLLVPASALHHLPDTVDDTAGAMVEPAGSALRAA
                     MAAAPAPGRRVLVWGLGPIGLLATQFALAANAEVHVVGLTNEARTFATTLGAHGAWAP
                     RDLPSLPWDAIIDATNGVDVPREALDLVEPGGRVVYIGLSGEPSTIDTRALVLKDVTA
                     VGILSGSPVVAGVIEEFASGRVDPRVLVGATVGLDRVADVLAGWRPDGAGIGPKIHVD
                     PRRTS"
     gene            2722..>3550
                     /locus_tag="VGD55_15370"
     CDS             2722..>3550
                     /locus_tag="VGD55_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0871779.1"
                     /translation="MPTAITDGRPIRWGILGAGGIADTVCTDIDMTPGNVVTAVAARD
                     GDRAAHFAAKHGAARSYGDYESLVRDDEVDVVYVTTTHPHHRAQALMAIDAGKSVLIE
                     KPVCLNATDAREVFAAATKADVFAMEAMWMTLNPLIRQAQQLVADGVIGDVRSVRSEF
                     GVGLPFDAAHRLYDLANGGGALLDLGVYPATFAFLFLGRPDEVQTVGTLAPTGADETV
                     AMQWLYEGHPGAQLWTSAAIAAPNGAAVLGTAGSIDVLPEAHRPSGLVVRNRDGEYRI
                     "
BASE COUNT          630 a         1171 c         1188 g          561 t
ORIGIN      
        1 agccccgcga cgctggtcgg aatggtcagc agataggtga agcgggccga gaccagggcc
       61 gcgatcacgc ctccagccag agcactcgcg gtcagtgcga cgctgttgag agcttgaatg
      121 cggccgtact gcttctcgaa gccgtcggcg tcccccgcct cttcgatcaa ggtgtcgtac
      181 acgatggcgt cgacggtgcc tgactgcatc gcgaagtaga cgccgagcag catcgcgctg
      241 gcgagatagg tggcgacgtt gtggctcagg ccaccgacga tcacgctggc cagcgacgcg
      301 aggctggcga gcatcaagac gccgcggcgg ctccaccggt cagcgaggat gcccgccgga
      361 atctcgagca gcggcacggt ggcgccgtag gcggcagcca tcagcgcgaa cagggttggg
      421 gtgaagccga gctgggtcat gaagatcttc tcgaccggca cccacggggc gatgccctgg
      481 agaaccagcg cgatccgaag cgggcggagc cgcgccgccg cggtggtaat accaggtgaa
      541 ctcgtcatgg cccgtacgac gaggcggtcc gcctgaacta atcgctggcg agcagatcgg
      601 tttcgggcag gatctcgggg agcccgaaca gcgggaacag gtcggtgtcg aaggtggtga
      661 tctcggcgag ttggcctgcc tcgaagcgca gcacatcgag cgacatcgcc cggaacaccg
      721 tgtcgccggg agcccggacg tagttcgctg cggccggatg ccgattcgct cgggtcgcaa
      781 cgagtctgaa ctcaccgacc gcccctgggg tgaatgtttc ggcgaagaac ttggcgagcg
      841 ctgccttgcc ctcgtacaag gttggctccg gtggcatcgt gaatcgcacg tcttcgcgca
      901 tcatcgccac caccgcgtcg ctgtcagcac gtgcgtgggc gtccatgtag cgctgaagga
      961 gcgcgcgctc ttcgaccgat ggctcagctg tcggcgtcca cgttgtgggt tcgtccgggc
     1021 ggtgctgctt caacgtggcg cgcgctcgtt gcaacgcact ggtgaccgac gcgacgctcg
     1081 tatcgagcag gtcggccgtt tcagcggcgg agaagtcgag cacgtcgcgc aggatgagca
     1141 ccgcgcgctg gtttggcgag agcaattgaa tcgccgtaag gtacgcgagc tcgatggtct
     1201 cgcgcgcgac gacgagcgcg tccggctcgt cagcgcgcga tgcttgccgg tccaaaagca
     1261 gatccggata gggctgcagc cacgggattt ccgatggcgc cgggtctccg ccgccgccga
     1321 cgagtaccac ccgctcagga tgagttcgca gcgtgtcgag acagacgttt gtcgcaatgc
     1381 gatacagcca cgcacgcatc ggcgagcgtc cctcgaacga cgcgcgattg cgccaggccc
     1441 gcaacagggc ctcctggacg tgatcctctg cgtcggtgaa ggagccgagc atccgatagc
     1501 agtgcacgcg caattcgctt cggtagcgat cagccacctg catgaacgcg gcgtcgtcgc
     1561 cgaccaaggt cgcatcatca gtcatgggca tcacgacgag aacgacgccg cgaactcatc
     1621 ggtattagga tgtccgtctg ggatcgacgt ggatcttcgg cccgatgcca gcgccgtcgg
     1681 gtcgccaccc ggccaacacg tcggcgacgc ggtcgagccc gaccgtcgcg ccgacgagca
     1741 ctctcggatc gacccgacct gacgcgaatt cttcgatcac gccggccacg acaggcgagc
     1801 cactcagaat gcccacggcc gtgacgtcct tcaggaccaa tgcccgggtg tcgatcgtgc
     1861 tcggctcacc cgacaggccg atgtagacga cgcggcctcc gggctcgacc agatcaagtg
     1921 cttcccgcgg gacgtcgacc ccgttggtcg cgtcgatgat cgcgtcccaa gggagcgaag
     1981 gcaggtcgcg cggagcccaa gctccgtgtg cgcccaacgt cgttgcgaat gtgcgagcct
     2041 cgttagtcag accgacgacg tgcacctcgg cgtttgccgc gagcgcgaac tgggtggcga
     2101 gtagcccgat cggcccgagc ccccagacaa ggacgcgccg acccggcgcg ggcgccgccg
     2161 ccatcgctgc gcgcaaggcg ctccctgcgg gttcgaccat ggcgccggcc gtgtcgtcga
     2221 cagtgtcggg aaggtgatgc agcgcgctcg ccggtaccag cagttgctcg gccagtgcgc
     2281 ccggccagcc gcgccggatg ccgatctcat atcggtcgac gcacacgtgt tggcgaccgt
     2341 tcaggcagcg gtggcagtga ccgcaaccga gcatcgtgtc gcccgtcact cggcgtccga
     2401 gccactctgc cggtacagcg tcaccgatcg ccgcgacctt gccacaccac tcgtggccca
     2461 gccgcaacgg gtacgccgcg tgcccttggt gcaggtaggc catcacgccc gtgaagaact
     2521 cggcgtcagt gccacagacg cccactcggt ggacgtcgac gaccacctcg ccaacgcccg
     2581 ccaccggagc ttcgacctcc tggacggcgc cctggtgcgg cccggtgacg acgaacgctc
     2641 tcacagggtc ggagactata gcctcgggtt cgaacgagac ggttcgaacg agacggttcg
     2701 aacgagatgg agagccgatc aatgcctaca gccataactg acggtcgacc gatccgctgg
     2761 ggcatcctcg gcgccggcgg catcgccgac accgtctgca ccgacatcga tatgacgccg
     2821 ggcaacgtcg tcaccgctgt cgctgcccgt gacggcgatc gagccgcaca cttcgctgcc
     2881 aagcacggag cggcacgtag ctacggcgac tacgaatctc ttgtgcgcga cgacgaggtc
     2941 gatgtggtgt acgtcacgac cacgcacccg catcaccgcg cgcaggccct catggcgatc
     3001 gatgcaggaa agtccgtgct gatcgagaag ccggtgtgtc tgaacgccac cgatgcacgg
     3061 gaggtgttcg ccgcagccac aaaagccgac gtgttcgcga tggaggcaat gtggatgacc
     3121 ctcaatccac tgatccggca ggcgcaacaa ctggtcgccg atggcgtgat cggggatgtg
     3181 cgaagcgtcc gcagtgagtt cggcgtcggg ctcccgttcg atgctgcgca ccggctctat
     3241 gacctggcca acggcggtgg tgcgctgctc gatctcgggg tttaccccgc cacgttcgct
     3301 ttcctgtttc taggtcggcc tgacgaggtc cagacagtag gcaccctcgc tccgaccggc
     3361 gctgacgaga cggtggccat gcaatggctc tacgaaggac accctggcgc gcaactgtgg
     3421 acctctgctg cgatcgcggc accaaacggc gccgcggtgc tgggaacggc gggctcgatc
     3481 gacgtgctgc cagaagcgca tcgaccaagc ggcctcgtgg tgcgcaaccg cgacggggaa
     3541 taccgcatcg
//
LOCUS       DASWVF010000872         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_279982, whole genome shotgun sequence.
ACCESSION   DASWVF010000872 DASWVF010000000
VERSION     DASWVF010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279982"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VGD55_15375"
     CDS             <1..1101
                     /locus_tag="VGD55_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0871780.1"
                     /translation="AAHPDTWPDVTPIGTGPFTVSPCTANNIQMVANPTYWAKGEPYI
                     QKVEYPAYLDNNPANLDLASGKAQWGSQFIPNIQQAYLSKSKDNNTWSPPVTNVEIFP
                     NLDPSHKATSTLAVRQAIALAIDRNQISTIGEGGQQPAANQTGVVVPTFQKYFDSDAL
                     AASGYDKPDVAKATQLMASAGYSTSKPLKLSIITITGYTDWDASLAVIKQELKPIGIN
                     LTVDDLAAQTYDDQLFNGNFDLAYYGEAGGPSPFYELRQILSSANTAPIGKQANSNYE
                     RYKNPAVDALFQQYPTADDAGQVAIIKQIEAAMLKDIPIIPTTESVDWFQYNTSDLGG
                     WPTKDNPYAQPAAFNVPDAEQVLLHLYSKSAQ"
     gene            1150..2139
                     /locus_tag="VGD55_15380"
     CDS             1150..2139
                     /locus_tag="VGD55_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871781.1"
                     /translation="MRFLLRRLGFFVLTLWAALTLNFFIPRMMPGSPLQALRDRTHNK
                     LSPAALNNLLAQFGFDPHQNVAIQYVDYLKNMATGHWGISIGGSLGEPVTKLIGQALP
                     WTLGLVGVTTVMAFLLGTVIGIVAGWKRGGLLDGIVPPVFVVTSALPYFWVALLMILL
                     FASGTHALLPNDGNYTNGIVPGFSGHFIYDVIKHAILPGATILVTAIGGWILTMRNNM
                     ITTLSEDYVRMGRAKGLSNRRIMFGYAARNAFLPNLSGFAMSLGFVISGAILVEYVFN
                     YPGLGYMLYNATVNTDYPLLQALFMLITVAVLIAVLICDFVTFWLDPRARAKA"
     gene            2145..>2503
                     /locus_tag="VGD55_15385"
     CDS             2145..>2503
                     /locus_tag="VGD55_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871782.1"
                     /translation="MSAILANTATVTGTATSPRRRAGGGLWTAVRSNRKAMAGLIIFV
                     IFLAMAAFPALFTSVKHPNDAAQYTSLAHPGSHHWLGTTQLGQDIYAQLIYGTRQSLV
                     IALLAGFFATVLSVLIGV"
BASE COUNT          477 a          865 c          712 g          449 t
ORIGIN      
        1 gccgcacacc ccgacacgtg gccagacgtg acgccgatcg gcaccggccc gttcaccgtc
       61 agcccgtgca ccgcgaacaa catccagatg gtggcgaacc cgacctactg ggccaagggc
      121 gagccctaca tccagaaggt cgagtacccc gcgtacctcg acaacaaccc ggccaacctc
      181 gaccttgcaa gcggcaaggc gcagtggggt agccagttca tcccgaacat ccagcaggcg
      241 tacctatcga agtcaaagga caacaacacc tggtcgccgc cggtgacgaa cgtcgagatc
      301 ttcccgaacc tcgacccgtc gcacaaagcc accagcacgc tcgcagttcg gcaggcgatc
      361 gctctcgcga tcgaccgcaa ccagatctcg accattggcg agggcggcca gcagccggcg
      421 gccaaccaga ccggcgtcgt cgtcccgacg ttccagaagt acttcgacag cgacgcacta
      481 gcggcctcgg gctacgacaa gcccgacgtt gccaaggcga cgcagttgat ggccagcgcc
      541 ggctactcga cgtccaagcc gctgaagctg tcgatcatca cgatcaccgg ctacaccgac
      601 tgggacgcgt cgctcgcggt catcaagcaa gagctcaagc cgatcggcat caacctgacc
      661 gtcgacgatc tcgccgctca gacctatgac gatcagctgt tcaacggcaa cttcgacctt
      721 gcctactacg gcgaggccgg cggcccgtcg ccgttctacg agctgcgaca gatcctcagc
      781 tccgcgaaca cggctccgat cggtaagcag gccaacagca actacgagcg ctacaagaac
      841 ccggcggtcg acgcgctgtt ccagcaatac ccgacggccg acgacgctgg gcaggtcgcg
      901 atcatcaagc agatcgaggc ggcgatgctg aaggacatcc cgatcatccc gacgacggaa
      961 tccgtcgact ggttccagta caacacctca gaccttgggg gctggccgac caaggacaac
     1021 ccctacgccc agccggccgc gtttaacgtg cccgacgctg agcaggtctt gctccacctg
     1081 tactccaagt cagcacaata agttctcact agcacagtcc atgagggtga gcgcgagggg
     1141 tgattcgacg tgaggttcct cctccgccgg ctcggcttct tcgtgctcac cctctgggct
     1201 gcgctgacct tgaacttctt catcccgcgg atgatgccgg gcagcccgct gcaggcgctt
     1261 cgcgacagaa cccacaacaa gttgtcaccg gccgcactga acaacctgct ggcccagttc
     1321 ggttttgatc cgcaccaaaa cgtcgccatt caatacgtcg actacctcaa gaacatggcg
     1381 accgggcact ggggtatctc gatcggtgga tcgctggggg agcccgtcac aaaactgatc
     1441 gggcaggcct tgccgtggac gctcggtctg gtcggcgtca ccaccgtcat ggcgttcctg
     1501 ctgggcaccg tgatcggcat cgttgcgggt tggaagcgcg ggggcctgct cgacggcatc
     1561 gtgccgccgg tcttcgtggt gacgtccgcg ttgccctatt tctgggtcgc gctgctgatg
     1621 atcctgctgt tcgccagcgg cacgcacgcc ctactgccca acgacggcaa ctacacgaat
     1681 ggcatcgtgc cgggcttctc tgggcacttc atctacgacg tgatcaagca cgcgattctc
     1741 ccgggcgcga cgattctggt cacggcgatc ggcggctgga tcctgaccat gcgcaacaac
     1801 atgatcacga cgctgtcgga ggactacgtc cggatgggcc gcgccaaagg actctcgaac
     1861 cggcggatca tgtttggcta cgcggcccgc aacgcgttcc tgccgaacct gtcggggttt
     1921 gcgatgtcgc tcggcttcgt gatcagcggc gcgattctcg tcgagtacgt cttcaactat
     1981 cccgggctcg gctacatgct ttacaacgcc accgtgaaca ccgactaccc gttgctccag
     2041 gcattgttca tgctcatcac cgtcgcggtt ctgatcgcgg tgctgatctg cgacttcgtg
     2101 acgttctggc ttgatccgcg cgcccgggcg aaggcgtagc gactatgagc gccattcttg
     2161 cgaacacggc cacggtcacc ggaactgcga cctcgccccg acgccgcgca ggcggtgggc
     2221 tctggaccgc ggttcgcagt aaccgcaaag ccatggctgg cctcatcatc tttgtgattt
     2281 tccttgctat ggccgctttc ccggcgttgt tcacgtcggt gaagcacccc aacgacgcag
     2341 cgcagtacac ctcgctcgcc caccctggca gccaccactg gctcggcacg acccaactcg
     2401 gccaggacat ctacgcgcaa ctcatctacg gcacgcgcca gtcgctggtc atcgccctgc
     2461 tcgccggttt cttcgccacc gttctctcgg tgctcatcgg agt
//
LOCUS       DASWVF010000873         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_432730, whole genome shotgun sequence.
ACCESSION   DASWVF010000873 DASWVF010000000
VERSION     DASWVF010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432730"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGD55_15390"
     CDS             complement(<1..438)
                     /locus_tag="VGD55_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEY0871783.1"
                     /translation="MRALLVTNPNATAMSSRTRDVIVRALGSELKLDVAETHSRGHAA
                     DLAAQARVDEFDLVVAVGGDGTVNECVSGLLRNGIGPDTPRLAVVPGGNANVFARALG
                     LPAEPIEATGAVLERLRSGRERRIGLGRLVTGELDRWFTFCAGF"
     gene            486..920
                     /locus_tag="VGD55_15395"
     CDS             486..920
                     /locus_tag="VGD55_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871784.1"
                     /translation="MPPQSDTAATDGSDRATDPSRPGVPLRLAVAATGVEALGLAASA
                     IGLTLYRLSGHRPFDMGDLWAVVAMAAIGAIGLGFVVRGLARAQRWARAPSVLTQLIG
                     LPIGVNAIGHGAAPFGIALVVCTLIGLVGLFAPSTSRRMSDL"
     gene            complement(931..1836)
                     /locus_tag="VGD55_15400"
     CDS             complement(931..1836)
                     /locus_tag="VGD55_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HEY0871785.1"
                     /translation="MNTRSPMPADDTTPSNATDATDATEPAVVAESTTDPIDAPVVGA
                     RAEQAASDRARARALFVTMVGLPEGSPERQAIRDQLVETHLPLVEYLARRFRNRGELH
                     DDLVQVATIGLIKSVDRFDLERGVEFSTYATPTIVGEIKRHFRDKGWAIRVPRRLQEL
                     KLSLAKATSELSQRNGRSPTVAELAAFLGMSDEDVLEGLESANAYSTVSLDTPESGES
                     DALAVADTLGVVDESLEGVEYRESLKPLLDQLPPREKRILLLRFFSNMTQSQIAIELG
                     ISQMHVSRLLARTLAQLREGLLVDE"
     gene            complement(1833..2252)
                     /locus_tag="VGD55_15405"
     CDS             complement(1833..2252)
                     /locus_tag="VGD55_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY0871786.1"
                     /translation="MSTPDGIPAEAPVRDVVSIRLPASGVYLAVLRTTTASLAARLDF
                     TLDEIEDLRIAVDEASALLIAGAVEGADLTSVFEMSTDSIHISVSTMTAEGSQAAPRD
                     SFAWTVLSALAGEVDAWTDPDGRQTISLVKHRSRDAT"
     gene            complement(2323..2835)
                     /locus_tag="VGD55_15410"
     CDS             complement(2323..2835)
                     /locus_tag="VGD55_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0871787.1"
                     /translation="MIRPAQPGDVPAILDLIRELATYEREPDAVVATEADLTAALFGE
                     QPAVFGLIAQDGDEVAGFALWFLNYSTWVGRHGVYLEDLYVRPEYRGHGHGRDLLLEL
                     AGIADERGYGRMEWSVLNWNEPAHRFYESLGAVPMQEWTTWRLTRSALASLTNGFDTR
                     SDAEGRATRD"
     gene            complement(2832..>3263)
                     /locus_tag="VGD55_15415"
     CDS             complement(2832..>3263)
                     /locus_tag="VGD55_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871788.1"
                     /translation="AIATPFPLGSYTSADCRRWLAGDPSARDCVTAMTADHARDFLIS
                     AAFCGMIGLFTIAAFTLLRRRWSSPAIATSLPRATEDVIGAVLAFGTAVVLVGQGIDA
                     VLVTRGNGAGEPFSLAPAALLAAAVFTVRARRRLRRPTLRA"
BASE COUNT          521 a         1038 c         1142 g          562 t
ORIGIN      
        1 gaagcccgcg caaaaggtga accagcgatc cagctcgccc gtgacgagcc tgccgagtcc
       61 gatccgacgt tctcgcccgg agcgcagccg ctcgagcacc gcaccggtcg cttcgatcgg
      121 ttcggccggt aggccgagag ctcgggcgaa gacattggca ttgccgcccg ggacaactgc
      181 caggcgtggg gtgtcgggcc cgatgccgtt gcgcagcagc cccgacacgc actcgttcac
      241 ggtgccgtca ccaccgaccg cgacgacgag atcgaactcg tcgactcgag cctgggccgc
      301 taggtcggcc gcgtggcccc gactgtgggt ttccgccacg tcaagcttca actcactgcc
      361 gagtgctcga acgatgacgt cgcgggtgcg cgagctcatg gccgtggcgt tgggattggt
      421 caccaacagc gctcgcattg cgaaagggta cgcctgcgac gggcgcgccg ggctatcttc
      481 gcctcgtgcc accgcagagc gacaccgccg cgaccgatgg gagcgatcgt gccacggacc
      541 cgtccaggcc cggcgtcccg ctacggcttg ctgttgccgc cactggtgtc gaggcgctcg
      601 ggcttgcggc gtccgccatt ggcttgacgc tttaccgctt gtcagggcat cgcccgttcg
      661 acatgggcga cctgtgggcc gtcgttgcca tggcggctat cggcgctatc ggcctgggtt
      721 tcgtcgtgcg cggtctggct cgcgcgcagc gatgggcgcg ggcgccgtca gtgctgactc
      781 aactgatcgg acttccgatc ggcgtcaacg cgatcgggca cggtgcggcg ccttttggca
      841 tcgcgctggt ggtgtgcacg ctcatcggtc tggtcgggct ttttgcgcct tcgacgtcgc
      901 ggcggatgag cgatctctag ggcggccgac ctactcgtcg actagcaagc cctcgcgcag
      961 ctgcgcaagc gtgcgggcga gcagtcgcga gacgtgcatc tgcgagatgc caagttcgat
     1021 cgcgatctgc gactgggtca tattgctgaa gaaccgcagg agcagaattc gcttctcgcg
     1081 cgggggcagt tgatcgagga gcggcttcag cgattcgcgg tactcgacgc cctcgagaga
     1141 ctcatcgacg acaccgagag tgtcggcgac ggccagcgcg tctgactcgc cgctctctgg
     1201 agtgtcgagg gagaccgtcg aatacgcgtt ggcggattcg agaccctcga gcacgtcctc
     1261 gtcgctcatg ccgaggaacg cggccaactc ggcaacggtc ggcgagcgcc cgttgcgctg
     1321 cgacagttca ctggtggctt tcgcgagcga cagcttgagt tcttgcagcc gacgcggaac
     1381 ccggattgcc cagcccttgt cgcggaaatg ccgcttgatc tcgccgacga tggtcggggt
     1441 cgcgtaggtc gagaattcga caccgcgctc gagatcgaaa cggtcgactg acttgatcag
     1501 cccgatcgtg gccacctgca cgagatcgtc gtgcagctcc ccgcggttgc ggaatcggcg
     1561 ggcgaggtat tcgaccaaag gcagatgggt ctcgacgagt tggtcgcgga tcgcttgtcg
     1621 ctcgggcgac ccttcgggca ggccgaccat ggtcacgaag agcgctcgcg cgcgtgcacg
     1681 atcgctcgca gcttgttcgg ctcgggctcc gacgaccggc gcgtcgatcg gatcggtcgt
     1741 cgattccgcg acgaccgcgg gctcggtggc gtcggtggcg tcggtggcgt tgctcggggt
     1801 cgtgtcgtcg gcgggcatgg gggagcgggt gttcacgtgg cgtcccgcga ccggtgcttg
     1861 accagcgaga tcgtctgccg cccgtcgggg tcggtccacg cgtcgacctc gcctgcgagc
     1921 gccgaaagca cggtccacgc gaacgagtcg cgtggcgctg cctggctgcc ttctgcggtc
     1981 atggtcgaca ccgagatgtg gatcgagtcg gtcgacatct cgaacacgct cgtgaggtcg
     2041 gcgccctcga cggcgccggc gatcagcaac gcagacgctt cgtcaacggc gatccgcagg
     2101 tcctcgatct cgtcgagggt gaagtcgagg cgggccgcaa ggctcgcggt ggtcgtgcga
     2161 aggaccgcga ggtacacccc ggaagcgggc agccggatcg acacgacgtc acgcacgggc
     2221 gcctcggcag gtatgccgtc gggggtcgac acgcttcgca ctccatctgc tcggaactgg
     2281 tctggccgga tcgccggttt gcgatggatg cacgctaccg cgctagtccc gggttgcccg
     2341 cccctcggcg tccgacctcg tatcgaaacc gttggtcaaa ctggcgagcg cgctgcgggt
     2401 gaggcgccac gtggtccatt cctgcatcgg gacggcgccc agtgattcgt agaagcgatg
     2461 cgcgggttcg ttccagttca ggaccgacca ttccatgcga ccgtacccgc gctcgtcggc
     2521 gatgccggcg agttcgagca gcaggtcgcg cccatgcccg tgaccgcggt attcgggtcg
     2581 cacgtagagg tcttcgagat agacaccgtg gcggcctacc caggttgagt agttcaggaa
     2641 ccacaacgca aagccggcaa cctcgtcacc gtcttgggcg ataagcccga acaccgccgg
     2701 ttgctcgccg aagagcgcag cggtgagatc ggcctcggtg gcgaccaccg cgtcgggttc
     2761 gcgctcgtag gtcgcaagct ctctgatgag gtccaagatc gcggggacgt cgccgggttg
     2821 tgctggtcgg atcacgctcg aagcgtaggc ctgcgcaatc ggcgtcgtgc tcggaccgtg
     2881 aacacggcgg ctgccaggag tgccgccggc gcgaggctga agggctcacc cgcgccattt
     2941 ccgcgggtga cgagcaccgc gtcgatgccc tgacccacga gtacgacggc agtgccgaac
     3001 gcaagaacgg caccaatgac atcttcggtt gctcgtggca gcgacgtcgc gattgcgggg
     3061 ctggaccatc gcctccggag gagggtgaac gcggcgatcg tgaagagccc gatcatgccg
     3121 caaaaggcag cgctgataag gaagtcgcgt gcgtgatcgg cagtcatcgc cgtgacacag
     3181 tcacgtgctg aagggtcgcc tgcaagccaa cggcggcaat cggcgctggt gtaggaaccg
     3241 aggggaaaag gggtcgcgat agc
//
LOCUS       DASWVF010000874         6884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_152382, whole genome shotgun sequence.
ACCESSION   DASWVF010000874 DASWVF010000000
VERSION     DASWVF010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152382"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="tilS"
                     /locus_tag="VGD55_15420"
     CDS             <1..975
                     /gene="tilS"
                     /locus_tag="VGD55_15420"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0871789.1"
                     /translation="VAAVRCAVRESLEQLSAVAPGSLVLVACSGGADSVALAAAAAFE
                     APRLGFRVGGVTVDHRLQEGSARRAAAVVTLLSKLGLDPVEAQPVDVPSGKSGPEAAA
                     RTARYAALDEVAERLGAASVLLGHTLDDQAETVLLGLARGSGTRSLAGMAAQQSRYRR
                     PLLGVTRASTRAACADLGLAVWDDPHNSDPAFSRVRVREQVLPMLETALGPGVAAALA
                     RTAGLARDDADALDSWAARSYTELADSEGSLDVARLAELPAAVRRRVLRLAALAAGTT
                     PGALSAVHLQAAEALITDWHGQGAVSLPGGRAAVRRYGRLYLGTDTVT"
     gene            1004..1567
                     /gene="hpt"
                     /locus_tag="VGD55_15425"
     CDS             1004..1567
                     /gene="hpt"
                     /locus_tag="VGD55_15425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY0871790.1"
                     /translation="MDQFDVSDDLAGILLSEKEIQAKVAELAAEIDADYAGKQLLLVG
                     VLKGAVMVMADLAREIHLPITVDFMAVSSYGSGGSSSGVVRILKDLDHGIEGRHVLVV
                     EDIVDSGLTLSWLVANLRSRGPASVEVCALMRKPDAIKQDVDVRYLGFDIPRDFVVGY
                     GLDYAEKYRNLPFVATLKRSVYDPQAG"
     gene            1732..3849
                     /gene="ftsH"
                     /locus_tag="VGD55_15430"
     CDS             1732..3849
                     /gene="ftsH"
                     /locus_tag="VGD55_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0871791.1"
                     /translation="MNVKRYARGPLFWVLLIVLFLVLLNGLWSGGSSYRSVSTSQVVA
                     NIASGNVSQAVFHDKEQTVQLTLKAPTAGKNPTTKIQASVAPDQELQLENQLAAVATQ
                     QKLDPTTLYTVKVSHTSLLLEILLNALPILLLVGLMIFMLTQMQGGGSRVMNFGKSKA
                     KLISKDTPKTTFADVAGADEAIEELVEIKEFLENPAKFQAIGAKIPKGVLLYGPPGTG
                     KTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFTQAKENAPAIIFIDEIDA
                     VGRHRGAGLGGGHDEREQTLNQLLVEMDGFDVKGGVILIAATNRPDILDPALLRPGRF
                     DRQIVVDRPDMEGRKGILRVHAKGKPFEGDVDLDVIARRTPGFTGADLANVINEAALL
                     TARHDRKQIDMLTLEEAIDRVMAGPERKSRIMGEKEKKIIAYHEGGHALVGHALPNAD
                     PVHKVTILPRGRALGYTLSLPTEDKFLVTRAEMMDQLAMLLGGRTAEELVFHEPTTGA
                     ANDIEKATSIARSMITQYGMSEKLGARKFGQENGEVFLGRDMGHQRDYSEEIASTIDD
                     EVRRLIESSHDEAWEILVEYRDVLDDLVLELMEKETLQKDEVLRIFARVQKRPHRGSY
                     TGYGKRQPSEKAPVLTPRELAVLASGGPDAGHGSNGSANGGSNGNSGSPSHAEPDGAG
                     SAGYSGSAGRADAGVDGSSPQTD"
     gene            3852..4493
                     /gene="folE"
                     /locus_tag="VGD55_15435"
     CDS             3852..4493
                     /gene="folE"
                     /locus_tag="VGD55_15435"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY0871792.1"
                     /translation="MTHLSVGSHDGPGGPNDTPVDDRVETFDLPRIEAAVREILAAIG
                     EDPTRDGLIETPRRVARAMAEQFAGLRERPEDVLTTVFDANHDEMIIVKDIEVYSTCE
                     HHLVPFHGVAHVGYIPNVKGEITGLSKIARLVDLFAKRPQVQERLTAQIADAMVEILE
                     PRGVIVVVECEHLCMSMRGVRKPGARTVTSAVRGAFRDSDRTRAEALRLISGR"
     gene            4693..6513
                     /locus_tag="VGD55_15440"
     CDS             4693..6513
                     /locus_tag="VGD55_15440"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEY0871793.1"
                     /translation="MTAARVEPQESTRRHPKEGDPFARVANERRSKRRWVPLVAGVLT
                     FLLGLRDIAMVVRPHLWERVKVIDHVLPGNVNPAVVHGAVGATLVVAGALLILLSHAL
                     RRRKRRAWRAVVGVLVLTLALHLLHGPESWQDMIDWLPADAALLIALLAFRGEFFARG
                     DPRTRWLALWAFLGLSAGSFTLGALLIEVRPGQVVGSVAFPSVAKHVIYGLVGVTGPV
                     QFRGDRTGDLVTLTLATMGAFTALTTVYLLFRPAEPRARLGADDEAQMRELLAKSGDL
                     DSLGYFALRRDKSVIWSATHKSCITYRVLSGVMLATGDPLGDPEAWPGAIKEFMIEAE
                     RHAWVPAVLACSELGGDVWTRETGMSALEFGDEAVVDVAEFSLDGRTMRNVRQMVNRV
                     ERAGYHSEVYRVRDLPVETAMTFRRQAESWRGAETERGYSMALGRLGDPADGDCVAVA
                     AMKDGVLRAFLHFVPWGTDGLSLDLMRRDRSAEPGLNEYLITTALRACPGLGVTRVSL
                     NFAVFRSALERGGRLGAGPVIKAWRGILVFASRWFQIESLYRFNAKFQPEWLPRYIVF
                     PGAGDLPRIAIAALEAEAFIVWPHPRIRQLRQLMRLGPAA"
     gene            6510..>6884
                     /locus_tag="VGD55_15445"
     CDS             6510..>6884
                     /locus_tag="VGD55_15445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0871794.1"
                     /translation="MTEPAVTAPRLVGFPDLGRCRVMGVVNVTPDSFSDGGHWFDADA
                     AIDHGHALIASGADIVDVGGESTRPGAQRVDADEELRRVLPVVGKLAASGVVVSIDTM
                     HAEVARRAVDAGAVLVNDVSGGL"
BASE COUNT         1103 a         2253 c         2353 g         1175 t
ORIGIN      
        1 gtggccgcgg tccggtgcgc tgtcagggaa tcgctcgagc aactgtccgc ggtagcgccg
       61 ggctcgttgg tactcgtcgc ctgtagcggc ggcgcagatt cggtcgcgct ggcggcagcc
      121 gcagccttcg aggcgccgcg actgggattt cgcgtcggcg gggtaaccgt ggatcaccgg
      181 ctccaggagg gctcggcgcg gcgggcggcc gcggtggtga cgctgctctc aaagctcggg
      241 ttagacccgg tcgaggcgca accagtcgac gtcccgtctg ggaagtctgg acccgaagcg
      301 gccgccagga ccgcgcgcta cgcggcgctc gacgaggttg ccgagcggct cggggccgcc
      361 agcgtcctgc tcgggcacac cctcgacgat caggccgaaa ccgtgctgct ggggttggcg
      421 cgcgggtcgg gaacccggtc gctggcgggt atggcggcgc agcagagcag gtaccggcgg
      481 ccgctgctcg gggtcacccg cgcttccact cgcgcagcgt gtgccgacct cgggctcgcg
      541 gtgtgggacg acccgcacaa tagcgatccg gcgttctccc gggttcgggt gcgggagcag
      601 gtgctgccga tgctggagac ggcgctcggg ccgggtgtcg cggcggccct ggccagaacc
      661 gcggggctcg cgcgcgacga cgcagacgca ctcgactcct gggcggctcg cagctacacc
      721 gagctggccg acagtgaggg atcactcgat gtcgcccgcc ttgcggagct cccggccgcc
      781 gtgcgacgca gggtcttgcg actcgccgcg ctcgccgcag gaaccacgcc gggcgcactg
      841 tcggcggttc acctccaggc cgcggaggcg ttgatcaccg actggcacgg gcagggagcg
      901 gtcagtttgc cgggcggtcg agccgccgtt cgcaggtatg gcaggctcta cctcggcacg
      961 gacaccgtta cctagtagac aaattcttcg aggggcgcac cgggtggacc agttcgacgt
     1021 cagcgacgat ctagccggaa ttttgctgag cgagaaagaa attcaggcca aagtcgccga
     1081 actcgcagcc gagatcgacg ccgattacgc cggtaagcag ctactgctcg tcggggtcct
     1141 caagggcgcg gtcatggtca tggccgacct cgcgcgcgag atccacctgc cgatcaccgt
     1201 cgacttcatg gccgtgtcct cctacggctc gggcgggtcg tcgtccggcg tcgttcggat
     1261 tttgaaagac ctcgaccacg gcatcgaagg ccggcacgtg ctggtggtcg aagacatcgt
     1321 cgactcgggg ctcaccctgt cgtggctggt ggcgaacctg cgttcgcgcg gcccggcgtc
     1381 ggtcgaggtg tgcgccctga tgcgcaagcc agatgccatc aaacaggacg tcgatgtgcg
     1441 ctacctcggc ttcgacattc cgcgcgattt tgttgttggg tacgggctcg actatgccga
     1501 gaagtaccgc aacctgccgt tcgtcgccac tctcaagcgt tcggtgtacg acccgcaggc
     1561 gggttagcga cgctgtcgcc gggaacgacc tgaggttgtc gcgggttaca cagggcggga
     1621 ctgcgctcgc tcggtgggtg ctcggtacgc gcgcggtgta ccgtcgaggg tggtcctagc
     1681 tgtcagattg cgtcgattcg gtaggaggga cggagctttc gctccgcgtc catgaacgtg
     1741 aaacggtacg cccgtggccc gctgttctgg gtcctcctca ttgtgctgtt ccttgtgctg
     1801 ctcaatgggc tgtggtcggg cggtagctcc tacaggtcgg tgtcgacgtc gcaggtcgtg
     1861 gccaacatcg cgtccggcaa cgtttcccag gcggtgttcc acgacaagga acagaccgtc
     1921 caactcacgc tgaaggcccc aactgccggc aagaatccga ccacgaagat ccaggcctcg
     1981 gtcgcgcccg accaagaact tcagctcgag aaccaactcg ccgccgtcgc gacccagcag
     2041 aagctcgacc ccacgacgct ctacacagtc aaggtcagcc acacgagcct gctgcttgag
     2101 atcttgctga acgccctgcc gatcctgctg ctcgtcggcc tgatgatctt catgctgacg
     2161 cagatgcagg gaggcggctc gcgggtcatg aacttcggca agtccaaggc gaagctcatc
     2221 agcaaggaca ccccgaagac gacgttcgct gatgtcgccg gcgctgacga ggcgatcgaa
     2281 gagcttgtcg agatcaagga gttcctcgag aatccggcca agttccaggc gatcggcgcc
     2341 aagatcccca agggtgtgtt gctttacggt ccgcccggta ccggtaagac gctgctggcc
     2401 cgcgcagttg ccggtgaggc cggcgtcccg ttctattcga tctcgggatc tgacttcgtc
     2461 gagatgttcg tcggtgtcgg cgcctcacgt gtgcgtgacc tgttcactca ggccaaagag
     2521 aacgctccgg cgatcatctt catcgacgag atcgacgccg tcggtcgcca ccgtggcgct
     2581 ggtcttggcg gcggtcacga cgagcgcgag cagacgctga accagctgct cgtcgagatg
     2641 gacgggttcg acgtcaaggg cggcgtcatc ttgatcgcgg ccaccaaccg gcccgacatc
     2701 ctcgaccccg cgttgctgcg ccccggccgg ttcgaccggc agatcgtcgt cgaccgtccc
     2761 gacatggaag gccgcaaggg catcctgcgc gtgcacgcca agggcaagcc gttcgagggc
     2821 gacgtcgacc tcgacgtcat cgcgcgccgc accccgggct tcaccggcgc cgacctcgcg
     2881 aacgtcatca acgaggcggc gctgctgacc gcccggcacg accgcaagca gatcgacatg
     2941 ctcacgctcg aagaggcgat cgatcgcgtc atggccggcc ccgagcgcaa gagccgcatc
     3001 atgggtgaga aggaaaagaa gatcatcgcc taccacgagg gtggtcacgc gctcgtcggc
     3061 catgcgctgc cgaacgccga cccggtgcac aaggtgacta tccttccgcg cggtcgggcg
     3121 ttgggctaca cgctctcgct gccgaccgaa gacaagttcc tggtgacccg cgccgaaatg
     3181 atggaccagc tcgccatgtt gctcggcggc cgcaccgcag aagagctcgt cttccacgag
     3241 ccgaccacag gcgccgccaa cgacatcgag aaggccacct cgatcgcgcg cagcatgatc
     3301 acccagtacg gcatgagtga gaagctcggc gcgcgcaagt tcgggcagga gaacggcgag
     3361 gtcttcctcg gccgcgacat gggccaccaa cgcgactact cagaagagat cgcatcgacg
     3421 atcgacgacg aggtgcgccg gctcatcgag agctcgcacg acgaggcctg ggagatcctc
     3481 gtcgagtacc gcgacgtgct cgacgacttg gtgctcgagc tgatggagaa ggagacgctg
     3541 caaaaggacg aggtgctgcg gatcttcgcg cgcgtccaga agcggccaca ccgcggctcc
     3601 tacaccggct acggcaagcg tcagccgtct gagaaggcgc cggtcctgac accgcgcgaa
     3661 ctcgcggtgc tcgcttccgg cgggcccgac gcagggcacg gcagcaacgg ctcggcaaac
     3721 gggggctcga acggtaactc cgggagcccg agccacgccg agcccgacgg cgctggctct
     3781 gctggctatt ccggctccgc cggacgtgcc gacgccgggg tcgacggctc tagtccgcag
     3841 acggactgac catgacgcac ctaagcgtcg ggtcgcatga cggacctggc ggtcctaacg
     3901 acacgcccgt cgatgatcgt gtcgaaacgt tcgatctgcc gaggatcgag gcggcggtcc
     3961 gcgagatcct ggccgcgatc ggcgaggacc cgacgcgcga cgggttgatc gagacaccaa
     4021 gacgagtcgc gagggcgatg gccgagcagt tcgccggcct tcgcgagcgc ccagaagatg
     4081 tgctgaccac ggtcttcgac gccaaccatg acgagatgat catcgtcaag gacatcgagg
     4141 tctacagcac ctgtgagcac cacctggtgc cgttccatgg cgtcgcgcac gtggggtaca
     4201 tcccgaacgt gaagggtgag atcaccgggc tttcgaagat cgcccggctg gtcgacctct
     4261 tcgctaagcg cccacaggtt caagaacgac tgactgccca gatcgccgat gcgatggtcg
     4321 agatcctcga acctcgaggg gtcatcgtcg tcgtcgagtg cgagcacctc tgcatgtcaa
     4381 tgcgaggcgt gcgcaagccg ggcgctcgta cggtcacatc ggccgtccgg ggcgccttcc
     4441 gtgactccga ccgcacccgg gctgaggccc ttcgacttat ttcgggccgc tgacgcgtcg
     4501 acgaatagcc gacgcacccc cagcccgctc caccgcacct actcttgatc cgtgacccga
     4561 gagccgagcc cagtgcgcga gaagatcgcc cggaaggtgg caccaagcgt gccgccgtgt
     4621 gcgaccgacg cgcctccggc ggcggctccg gcagcaaccc cagctgcagc cctggctgag
     4681 gatgcggccc tggtgaccgc cgcccgagtc gagccccagg agtcgacccg ccggcacccc
     4741 aaagagggcg atccgttcgc ccgggtggct aacgagcgac gctcgaagcg gcgctgggtg
     4801 ccgctggtgg ccggcgtcct gaccttcctg ttgggcctgc gagacatcgc gatggtggtc
     4861 cggccgcacc tgtgggagcg cgtcaaggtc atcgatcatg tgctgccggg caacgtcaac
     4921 cctgcggtcg tgcacggcgc cgtcggcgcg accctcgtcg tcgctggcgc gttgttgatc
     4981 ctgctctcgc atgcgttgcg ccgtcgtaaa cgacgcgcgt ggcgagcagt ggtcggcgtc
     5041 ctcgtgctaa cgcttgcact gcacctcctg cacgggcccg agtcgtggca ggacatgatc
     5101 gactggctgc ccgccgatgc tgctctgctc atcgccttgc tcgcgtttcg tggtgagttc
     5161 tttgcccgtg gcgatccgcg taccaggtgg cttgcgttgt gggcattcct cgggctgtcg
     5221 gccggcagct tcacgctcgg cgcgctgctc atcgaggtac ggccaggtca ggtcgtcggc
     5281 tcggtggcgt tcccgagcgt tgccaagcac gtgatctacg gcctcgtcgg tgtcaccggg
     5341 cccgtgcagt tccgtggtga ccgcacgggc gacctcgtca ctttgacgct ggccacgatg
     5401 ggtgcgttta cggcgctgac gaccgtgtac ctgctgttcc ggccagccga accacgcgcc
     5461 cggctcggcg ccgacgacga agcccagatg cgtgagctgc tcgccaagag cggcgacctc
     5521 gattcgctcg gttacttcgc gcttcggcgc gacaagagcg tcatctggtc ggcgacgcac
     5581 aagtcgtgca tcacctaccg agtgctgtcg ggagtcatgc tcgcgacggg tgatccgctg
     5641 ggagaccccg aggcatggcc tggtgccatc aaggaattca tgatcgaggc agaacgccat
     5701 gcctgggtgc cggcggtgct tgcgtgcagt gaactcggcg gcgacgtctg gactcgcgag
     5761 accggaatgt cggcgctcga gttcggcgac gaggccgtcg tcgatgtcgc cgagttcagc
     5821 ctcgacgggc gcacgatgcg caatgtgcgg cagatggtca accgcgtcga gcgggccggc
     5881 tatcactccg aggtctaccg ggtacgcgat cttcccgtcg agaccgccat gacgtttcgg
     5941 cggcaggcgg agtcgtggcg cggcgccgaa accgagcgcg gttactcgat ggctctgggt
     6001 cgcctcggcg atccggccga cggagactgc gtcgcagtag cggcaatgaa ggacggcgtg
     6061 ttgcgggcgt ttctgcactt cgtcccgtgg ggcaccgacg gtctctctct cgacctgatg
     6121 cgccgcgacc gcagtgccga gccggggctg aacgagtacc tgatcacgac tgccttgcgc
     6181 gcgtgtcctg gcctcggggt gacccgcgtt tcgctgaact ttgctgtctt ccgctcggct
     6241 ctcgagcgcg gtggccgtct tggcgccggc cccgtcatca aggcgtggcg agggattctc
     6301 gtcttcgctt cgcgctggtt ccagatcgag tcgctctacc gcttcaacgc gaagtttcag
     6361 cccgagtggc tgccgcgcta catcgtcttc ccaggtgctg gtgacctgcc gcgtatcgcg
     6421 atcgcggcgc ttgaggcgga agcctttatc gtgtggccgc acccgcggat tcggcagctg
     6481 cgccagctga tgcggctagg cccggcggcg tgaccgaacc ggctgtgacc gcgccacggt
     6541 tggtcggatt ccctgacctg ggcaggtgcc gggtgatggg cgtcgtgaac gtgacaccag
     6601 actcgttctc tgacggcggc cactggttcg acgccgacgc tgcgatcgat cacggtcacg
     6661 cgttgatcgc gagtggcgcc gacatcgtcg acgtcggggg tgagtcgacg cggccgggtg
     6721 cgcagcgcgt cgacgccgac gaggagctgc gcagggtgct tccggtcgtg ggcaagctcg
     6781 cggcttccgg cgtcgtcgtg agcattgaca ccatgcacgc ggaggtggcc aggcgtgcag
     6841 tcgacgccgg cgcggtgctc gtcaatgacg tcagtggcgg attg
//
LOCUS       DASWVF010000875         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_280118, whole genome shotgun sequence.
ACCESSION   DASWVF010000875 DASWVF010000000
VERSION     DASWVF010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280118"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGD55_15450"
     CDS             complement(<1..587)
                     /locus_tag="VGD55_15450"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0871795.1"
                     /translation="MTAPVEKPQPVEKAWRARGSRLHPLTPVVRAGRVLVSMAALAAY
                     NIKDAKHAGWWLPVIVVAVLLLGFGAGVASWAFTRFRIDGGEVHIDSGVFVRRSRQVR
                     IDRLQAIDIVQPLIARAWGLGELKLDMAGGAEGRVRLGYLRLEEAQGLRASLLAMAAG
                     LDHRTPEAPQQALLHCSGGAIVGSALLSTQAFIGF"
     gene            complement(584..1072)
                     /locus_tag="VGD55_15455"
     CDS             complement(584..1072)
                     /locus_tag="VGD55_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0871796.1"
                     /translation="MQQAFAPPGETWVGVSPTLRAMRRTLIGVVAGLLAVGLAIAFVA
                     AGSVVAAVMAVVGIVLFAAAGFIIVGRNYASWGYAERGADLLVTHGVLIRRLVVVPYG
                     RMQLVDVTAGPIERKFGISTLRLHTASAGTDARIRGLTAAEANRLRDRLAALGESQSA
                     GL"
     gene            complement(1185..1631)
                     /locus_tag="VGD55_15460"
                     /pseudo
     CDS             complement(1185..1631)
                     /locus_tag="VGD55_15460"
                     /inference="COORDINATES: protein motif:HMM:NF022819.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
     gene            complement(1628..2125)
                     /gene="folK"
                     /locus_tag="VGD55_15465"
     CDS             complement(1628..2125)
                     /gene="folK"
                     /locus_tag="VGD55_15465"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0871797.1"
                     /translation="MKAVLGLGANVGDRAVTLQAAVDALARETTVVAVSAIFETVPVG
                     GPPQPDFYNAVVIVETALSALELLDLAHRLESAAGRVRLEHWGPRTLDVDVLAYDAVR
                     SDDPQLTLPHPRAHERAFVLLPWLDVDPEAYVEGVGKVADLVATVETTGVRRLVAPTL
                     RWGVT"
     gene            complement(2122..2490)
                     /gene="folB"
                     /locus_tag="VGD55_15470"
     CDS             complement(2122..2490)
                     /gene="folB"
                     /locus_tag="VGD55_15470"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY0871798.1"
                     /translation="MTDRIALRGLVVRGHHGVYEHERRDGQDFVVDVALDVDTSVAAA
                     TDDVSDTVDYGTVATAIAAVVGGEPVNLLETLAQRIASACLVDVRVMSVEVSVHKPQA
                     PIPLTFGDVAVTIVRSRGDR"
     gene            complement(2497..>2729)
                     /locus_tag="VGD55_15475"
     CDS             complement(2497..>2729)
                     /locus_tag="VGD55_15475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0871799.1"
                     /translation="ILMELGRPVLIGASRKAFLGRLLAVGDEPRPPAERDYASSAITA
                     LAAAAGAWGVRVHDVVADVDAVRVAARWRDQP"
BASE COUNT          499 a          957 c          890 g          383 t
ORIGIN      
        1 atgaacccga tgaaggcctg ggtcgacagc agcgcactgc cgacgatcgc gccgcccgag
       61 caatgcagga gcgcctgctg tggcgcctcg ggcgtgcggt ggtcgagacc ggccgccatc
      121 gctagcagcg aagcgcgtaa accctgcgcc tcctcgagcc gcaggtagcc aagccgcacc
      181 cgcccctcag cgccaccggc catatcgagc ttcaactccc ccagccccca cgcgcgcgcg
      241 atcaacggtt gcacgatgtc gatggcttgg agacgatcga tgcgaacctg acgcgacctt
      301 ctgacgaaca cccctgaatc gatgtgcact tcgccgccgt cgatgcggaa tcgcgtgaaa
      361 gcccacgatg cgacacctgc gccgaagccg agcaggagca ctgccaccac gatgaccggc
      421 agccaccagc cggcgtgctt tgcgtccttg atgttgtagg ccgcaagggc agccatcgac
      481 accagaactc ggcccgctct gacaacgggc gtcagcggat gcaggcggct cccgcgcgcc
      541 cgccaggctt tctcgacagg ctgcggtttc tcgacgggcg cggtcacaac ccggccgact
      601 gggattcccc gagcgccgca aggcggtcgc gcaacctgtt ggcctcggcg gcggtgagcc
      661 cgcggattcg cgcgtcagta ccggccgacg cggtatgcaa gcgcagcgtc gaaatcccga
      721 acttgcgttc gatcggaccc gcagtcacat cgacaagctg catacgccca tacgggacga
      781 cgaccagccg gcgtatcagc acgccgtggg tcaccagcag gtcggcgcca cgctccgcgt
      841 atccccacga ggcatagttg cggccgacga tgatgaaccc ggccgccgcg aacagcacga
      901 tcccgaccac cgccatgacc gcagcgacca ccgagcctgc tgccacgaaa gcgatcgcga
      961 gcccgaccgc gagtaacccg gcgaccacgc cgatcagcgt gcgacgcatg gcgcgcagcg
     1021 ttggtgagac gccgacccac gtttcccccg gcggtgcgaa tgcctgttgc atgcgtgtca
     1081 cactacggcc ggggccagac caacaggcag cgcgtctcgg cggggcgcgg ccgaaccggg
     1141 attgtggagt accgacgttg ttctaggtgc gtccagactc cacgttattc gcggtcgtcg
     1201 ggcggtcggg gtacccggca gctgtactcg aggaacagcg cggcgccgac cagcgcgagc
     1261 gaggcaacga cgctcagacc gctggcacgg ctgtcggcgt tgatcctttc cttgccgagg
     1321 ttgctcagcg tgtagacgag gaatccgccg tacaggccgg cgacaatcgc cgccgcgtgg
     1381 ctgctcgctt tcgcgagcac cgcgagcttc gcaatgaaca gcggctagat cggccggtcg
     1441 cccggatccc gcaatcgcgc acgcaccgag aacgcgtaca tcagctcgaa cagcgacaac
     1501 acaacgagcg tgacgggccc gacgatgggc agcgcgggca gcgagctgta cgactcgcgc
     1561 acgacgagcc agctcagcac gccgaacccc aatgcgagcg ccgcgaggat cgacgagcgg
     1621 gtcggcatca tgtgacgccc caacgcaacg tcggtgcaac gaggcgccgc acgccggtcg
     1681 tctcgacggt cgccaccaga tcggcaactt tgccgactcc ttcgacgtac gcctcgggat
     1741 cgacgtcgag ccacggcagc agcacgaaag cgcgctcgtg cgcacgtgga tgcggcaatg
     1801 tcagctgcgg gtcgtcgctg cgcactgcgt cgtaggcaag cacatcgacg tccagcgtcc
     1861 gtgggcccca atgctcaagc cgcacccggc ccgccgctga ctcgagacgg tgcgcgaggt
     1921 cgagcagttc gagggcactc agggcggtct cgacgatgac gaccgcgttg tagaaatcgg
     1981 gctgcggcgg accgccgacg ggcacggtct cgaagatggc tgacacggcc acgaccgtgg
     2041 tttcgcgagc aagcgcgtcg accgcggcct gcaaggtgac agcccgatcg ccgacgttgg
     2101 cgccgagccc gagcaccgcc ttcaccggtc gcctcgcgac cgcacgatgg tgaccgccac
     2161 gtcgccgaat gtcagcggga tcggcgcctg cggcttgtgc acactcactt cgacagacat
     2221 gacgcggacg tcgaccaggc aggccgatgc gatgcgctgc gccaacgtct cgagcaggtt
     2281 cacgggttcg ccgccgacaa cggctgcgat ggccgtagca acagtgccgt agtcgacggt
     2341 gtcgcttacg tcatcggtag ccgccgcaac cgacgtgtcg acgtcgagcg cgacatcgac
     2401 gacgaagtct tggccgtcgc ggcgctcgtg ttcgtaaact ccgtgatgtc cgcgcacgac
     2461 aagcccgcgc agcgcgatgc gatcagtcat gactgctcac ggttgatcgc gccaccgtgc
     2521 agcaacccgc acggcgtcga cgtcggcaac gacatcgtgc acccgcacgc cccatgctcc
     2581 cgcggcagcg gcaagtgccg tgatcgcaga gctggcgtag tcgcgttcgg ctggtggccg
     2641 tggctcgtcg cccacggcga gcaagcgtcc gaggaacgct ttgcgcgacg ctccgatcag
     2701 cacggggcga cccagctcca tgaggatgt
//
LOCUS       DASWVF010000876         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_177605, whole genome shotgun sequence.
ACCESSION   DASWVF010000876 DASWVF010000000
VERSION     DASWVF010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177605"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(669..>1904)
                     /locus_tag="VGD55_15480"
     CDS             complement(669..>1904)
                     /locus_tag="VGD55_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0871800.1"
                     /translation="GLWSSFAQGNAGRGQVELMVRGTDDTGTSVGASQSFHLQFSKDD
                     IKGALYYWTTSAKTAIMRWDFGGSTKTAAQYLTPANTDGKTCVGCHALSLDGTKLVAS
                     AGGQNDGRLLLWNVSTNTPQQPFPLMHKSQFESWSADGGQFVGIYGDSNPNHTGPVNL
                     MIFDGNTGAVTKTIDLGGLRADHPDWSKNTTGRNTIAFTSADSAAPTTDQRPATGGID
                     FIQLNGTTWSPPQVLVPAVLGKNHYYPAIAPDGNLVVYDESTCTNGTPMAGQAPDKSC
                     DADTDMTATIFLTSLAGATPVALTNANSPGVADMGAVALTNSFPKWAPFIEQLDELHQ
                     VMWLTFSSTRQYGLRSPPPPANTSETTKGTLIWMVGMVLGPGGSDPSFTAFCLPFQDI
                     ATSNHIAQWAQTFVPIPIN"
BASE COUNT          345 a          558 c          643 g          358 t
ORIGIN      
        1 acaacttgaa atctcaggtt gcggcctggc ggcggcgcgc cgaacacgcc ggccgcccca
       61 tccgctggaa attcaccgtt tcagatgcga ggcgcgtctt ccggtacgac ggaatcatca
      121 cgcctcggac gcggcactag cagagattga ctgactcccg gtgttggcgc gatcgcgtta
      181 gggcgacaga aagcatgttc cggccggtac gggcgctcgt ttcccgcgtg gtccaggcgc
      241 tcgtgccgtt gattcggcgc gcggcgatcc ttagcgcgcg agcttgaagc ggcactgagc
      301 gaggaccgaa ggccgaaacc agaaaagaag ggcgacatgt cgggcgaggc agcaatcgtc
      361 gccctaacgt ggagcctgcc gtcaccagag cggtgaaggt ggaccaacgc cgacgcggcg
      421 gtgtcttcag gccaccggtg acgagtcgac ggctcgattg tcacgcagcc gagcgcagca
      481 gcaccctggc gagcgccgtc gactgagcga gcagccctat cgagagttcc ggaccgcggt
      541 cgtcctcggc aagtgccgca cgttcgtggc aaccgtcgcc gtcggtgcgg gggacggacc
      601 cgtgtacgga cgcggacgaa ggccgtcggg cgtcgaggtc gatctcgccg actcgggccg
      661 ggcggaaatc aattgattgg aatcggaacg aaggtctgag cccactgagc aatgtggttc
      721 gacgtcgcga tgtcctggaa cggaagacag aaggctgtga agctcgggtc ggaaccgcca
      781 gggccgagca ccatcccaac catccagatg agtgtcccct tagtcgtctc actcgtgttg
      841 gcgggaggtg gcggtgagcg aagcccgtac tggcgggtcg acgagaaagt tagccacatg
      901 acctgatgta gctcgtcgag ttgctcgatg aatggcgccc atttggggaa agagttcgtc
      961 agcgccacgg cgcccatgtc cgcgacgcca gggctgttgg cgttggtcaa ggctacgggc
     1021 gtggctccgg ccagcgacgt caaaaagatg gtcgctgtca tgtccgtgtc cgcgtcgcaa
     1081 gacttgtcag gcgcctgacc ggccatcggc gttccgttcg tgcaggtaga ctcgtcgtaa
     1141 acgacgagat tgccgtctgg ggcgatggct gggtagtagt ggtttttgcc cagcaccgcc
     1201 ggcacgagca cctggggtgg actccaggtc gtgccattga gctggatgaa gtcgatgccg
     1261 cccgttgcag gacgctgatc ggttgtggga gccgccgaat ccgcagaagt gaaggcaatc
     1321 gtgttccggc cggttgtgtt cttggaccag tctgggtggt cggcgcgcag tccaccaagg
     1381 tcgatcgtct tcgtgaccgc accggtgttg ccgtcgaaga tcatgaggtt cactgggcct
     1441 gtgtgattgg gatttgagtc gccgtagata ccgacgaact ggccaccgtc ggcactccac
     1501 gattcgaact ggctcttgtg catcagaggg aagggctgct gtggcgtgtt cgtcgacaca
     1561 ttccagagca gcagtcgacc atcgttctga ccacccgcgc tggccaccaa cttcgtgccg
     1621 tcgagcgaga gagcatggca cccaacgcaa gtcttcccgt cggtgttggc aggcgtcaag
     1681 tattgggcgg ccgtcttggt gctcccgcca aaatcccacc gcataatcgc ggtcttggcg
     1741 ctcgtggtcc agtaatagag ggcgcccttg atgtcgtctt tcgagaactg caggtggaac
     1801 gattggctgg ctccgacgga ggtgccggtg tcgtcggttc ctcggaccat gagctcgact
     1861 tgacctcgac ccgcgttccc ctgagcgaag ctcgaccaca ggcc
//
LOCUS       DASWVF010000877         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_126985, whole genome shotgun sequence.
ACCESSION   DASWVF010000877 DASWVF010000000
VERSION     DASWVF010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126985"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1040)
                     /gene="speB"
                     /locus_tag="VGD55_15485"
     CDS             complement(21..1040)
                     /gene="speB"
                     /locus_tag="VGD55_15485"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0871801.1"
                     /translation="MFGPDVTFLGVDRCDLADPTSFAGADVVIVGAPFDGGTSHRPGT
                     RFGPQAIRSTDYLPHDGSRPHLALRVDALRDLRVLDAGDVEMPPGEIERSLAALEAAV
                     ATVAGSGAIPVVLGGDHSIALPDVTGVARHFGFGRVAVLHFDAHADTGDFVFGSLYGH
                     GQPMRRLIESGAARGDRFLQIGLRGYWPEPRTLDWMAAQRMRSYEMTEIVARGLPAVL
                     DEAFEIALDDCEAVFLSVDIDVCDPGHAPGTGTPEPGGLTARELLDAVRRICRELPVA
                     GVDVVEVSPPYDHAEITAMLANRVVLEALSGIAARRRDATTAIPMNPAMPLLAGLHDG
                     PARHS"
     gene            1238..>1594
                     /locus_tag="VGD55_15490"
     CDS             1238..>1594
                     /locus_tag="VGD55_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871802.1"
                     /translation="MVAESTQVHYDNGEVLDKGLKGGALGLVSSIVIGVASTAPAYSL
                     AATLGFVVLLAGTQSVIVSILAFVPIFLVSLGYKELNRREPDCGTTFTWATKAFGPKT
                     GWLGGWGIIAADVLVMA"
BASE COUNT          307 a          526 c          507 g          254 t
ORIGIN      
        1 atcttcatga cgatgtccct tcaggagtgt cgtgcaggac catcgtgtag gccggcgagc
       61 aagggcatcg ccgggttcat cgggattgca gtagttgcat cgcggcgcct cgccgcaata
      121 cctgacaagg cctccaacac aacccgattc gcgagcatcg cggtgatctc ggcgtggtcg
      181 tagggcggtg aaacctcgac cacatcgacg cccgcgacgg gcagctcgcg acagatccgg
      241 cgaacggcat cgagcagttc gcgcgccgtc agtccgcccg gttcaggagt accagtgccg
      301 ggggcgtgac ccggatcgca cacgtcgatg tcgacagaca ggaacaccgc ctcgcagtcg
      361 tcaagcgcga tctcgaatgc ttcgtccagg acggcgggca gcccgcgggc cacgatctcg
      421 gtcatctcgt acgaccgcat ccgctgtgcc gccatccagt cgagagtccg cggctccggc
      481 cagtagcccc gcaggccgat ctgcagaaac cggtcaccgc gcgcggcacc ggattcgatg
      541 agccgccgca tcggttggcc gtggccgtac agagatccga agacgaagtc gccggtgtcg
      601 gcatgcgcat cgaagtgcaa aacggcgacc cgaccgaatc cgaagtgccg agcaactcct
      661 gtgacatcgg gcagcgcgat cgagtggtca cccccaagga caaccggaat tgcgccagaa
      721 cccgcgacgg tagcgactgc tgcttccaat gccgccaagg agcgctcgat ctcgccgggc
      781 ggcatctcca catcgccggc gtcaagaacc cgcaggtcgc ggagcgcgtc gacgcgcagt
      841 gccaggtgcg gccgggatcc gtcgtgcggc aggtagtcgg tcgaacggat cgcctgcggg
      901 ccgaatcggg tacccgggcg atgcgatgtc ccgccgtcga acggtgctcc gacgatgacg
      961 acatcggctc ccgcaaacga tgtcgggtca gcgagatcgc aacgatcgac gcccaaaaag
     1021 gtgacgtccg ggccgaacat cgccccatac cgggtcactt cacaccggta gccgaccggc
     1081 aggcaacgct tgcatggcgg gacgctacca ccgaaacggt cgcgctatca gaacgagacc
     1141 acggaatccc ttgtcatccg acgaaatcag tgcgataacc ggagggttca aaaccgctgg
     1201 tgccctcagt ccgcccccgt cgctcccaga ggtgctcatg gtcgccgagt caacacaggt
     1261 tcattacgac aatggagagg tcctggacaa agggctcaag ggcggtgcgc tcgggcttgt
     1321 ctcgagcatc gtcatcgggg tcgcatcgac ggcgccggcg tacagcctgg ccgccacact
     1381 cggcttcgtc gtcctgctcg cagggacgca gtcagtaatc gtgtcgatcc tcgccttcgt
     1441 gccgattttc ctggtctcac tgggttacaa ggaactgaac cgacgcgagc ccgactgcgg
     1501 caccacgttc acgtgggcca ccaaagcctt cggcccgaag accggttggc ttggtggctg
     1561 gggcatcatt gcggctgacg tgctggtcat ggcc
//
LOCUS       DASWVF010000878         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_432991, whole genome shotgun sequence.
ACCESSION   DASWVF010000878 DASWVF010000000
VERSION     DASWVF010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432991"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="proB"
                     /locus_tag="VGD55_15495"
     CDS             complement(<1..1037)
                     /gene="proB"
                     /locus_tag="VGD55_15495"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0871803.1"
                     /translation="MPASTEGVARPVVAAARRIVVKIGSSSLTRPEGGLDQANIDALA
                     AAIVGRLGQGTSVVLVSSGAIAAGLRPLGLTARPRDLATQQAAASVGQGLLVESYARA
                     FGLHDVRVGQVLLTSDDMIRRVHYLNAERTLTRLLELRVVPVVNENDTVVTDEIRYGD
                     NDKLAALVAHLVRADLLVLLSDVDALYTAPPGKPGSRRLDDVHGEADLADVDLGGVGR
                     VGIGSGGMVTKVDAARLATSAGIPVVVASAAAVVEALAGKHAGTLFHPTGARVASRLL
                     WLAHATTPRGRLVLDPGAVAAVVDRRLSLLPAGITSVEGEFTAGDPVDLVDQEGRAVA
                     RGLVNYDATELP"
     gene            complement(1043..>1880)
                     /gene="obgE"
                     /locus_tag="VGD55_15500"
     CDS             complement(1043..>1880)
                     /gene="obgE"
                     /locus_tag="VGD55_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0871804.1"
                     /translation="AALSAARPKIADYPFTTLVPNLGVVQAGESRFTVADVPGLVRGA
                     SEGRGLGLEFLRHVERCSALVHVIDCATMEPDRDPVSDLEAIEAELAAYNETDVGGVP
                     LNERPRLVALNKVDVPDARDLAEMVRAEIEARGIKVIIVSAATREGLRELSFAMAALV
                     EQARAARPTEENTRIVLRPRAVDDSGFTVTKVGTSFVVKGDRPERWIKQTDFTNDEAV
                     GYLADRLNRLGVEAELAKAGAEAGAEVVIGDVAFDWEPGMLAGSEHRIGPRGTDSRLD
                     GR"
BASE COUNT          304 a          668 c          622 g          286 t
ORIGIN      
        1 ggcaactcgg tcgcgtcgta gttcacgagg ccccgtgcta cagctcggcc ttcttggtcg
       61 acgaggtcga ccgggtcacc ggcggtgaac tcgccttcga ccgaggtgat gcctgcgggc
      121 agcaacgaca agcgccggtc gacgaccgcc gcgaccgcgc cagggtcgag aaccagccga
      181 ccgcgcggtg tcgtcgcatg ggcaagccac agcagccggc tcgccactcg cgccccggtc
      241 ggatgaaaga gcgtgccggc gtgctttccg gccagcgcct cgaccaccgc cgcggctgag
      301 gcgacgacca cggggatgcc tgccgatgtc gccagcctgg ccgcgtcgac cttggtgacc
      361 atgccgccgg acccgatacc gactcgcccg acgccaccga ggtcgacgtc ggcgagatca
      421 gcttcgccat gcacgtcgtc gaggcgccgc gagcccggct tccccggcgg agccgtgtac
      481 agcgcgtcga cgtcggagag cagcacgagc aggtcggctc gaacgagatg agcgaccagg
      541 gccgcgagct tgtcgttgtc gccgtagcgg atctcatcgg tcacgacggt gtcgttttcg
      601 ttgacgaccg gcaccactcg caactcgagc agccgggtca gcgtgcgctc tgcgttgagg
      661 taatggaccc gtcggatcat gtcgtcagag gtcagcagaa cctggcccac gcgcacatcg
      721 tgcagcccga acgcgcgcgc gtatgactcg acgagcagcc cttgcccgac actcgcagcc
      781 gcctgttggg tggcgagatc gcgcggcctg gcggtgagtc cgagcggccg caggccggcg
      841 gcgatcgcgc ccgacgacac cagcacaacc gacgtcccct gaccgagacg cccgacgatt
      901 gcggccgcga gcgcatcgat gttcgcctgg tcgaggccgc cctcaggccg ggtcagtgac
      961 gaggacccga tcttgacgac gatcctgcgc gcggccgcga cgaccggccg ggcaacgccc
     1021 tccgtacttg ccggcacgtc gatcaccgcc cgtcgaggcg cgagtcggtt ccgcgcggtc
     1081 cgatccggtg ctcagacccg gccagcatcc cgggctccca gtcgaacgcg acgtcaccga
     1141 tgacaacctc ggcgccggct tcggcgccgg ccttcgcaag ttccgcctcg acaccgagcc
     1201 ggttcagccg gtcggcgagg tacccgactg cctcgtcgtt ggtgaagtcg gtctgcttga
     1261 tccaacgctc gggacgatcg cccttgacca cgaaactcgt gccgaccttg gtgaccgtga
     1321 agccgctgtc gtcgaccgcg cgcggtcgaa gcacgatgcg ggtgttttct tcagtcggcc
     1381 gtgcagccct ggcctgctcg accaacgcgg ccatcgcgaa cgagagctca cgcaggcctt
     1441 cgcgggtcgc ggccgacaca atgatgacct tgataccgcg cgcttcaatc tcggctcgaa
     1501 ccatctcggc aagatcgcgc gcgtcgggca cgtcgacctt gttcagcgcg accaaccgtg
     1561 gccgctcgtt cagcgggaca ccgccgacat cggtttcgtt gtaggcagcg agctcggctt
     1621 cgatcgcttc gaggtcactc accggatcgc ggtcgggttc catcgtggcg cagtcgatga
     1681 cgtgcacgag tgcgctgcag cgctcgacgt gtcgcaagaa ctcaaggcca agaccgcggc
     1741 cctcacttgc gccccgaaca aggccaggaa cgtcggccac ggtgaagcgc gactcccccg
     1801 cctgcacgac gccgagattt gggaccagcg tggtgaacgg atagtcggcg atcttcggcc
     1861 tggccgccga cagcgccgcg
//
LOCUS       DASWVF010000879         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_305411, whole genome shotgun sequence.
ACCESSION   DASWVF010000879 DASWVF010000000
VERSION     DASWVF010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305411"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..639
                     /locus_tag="VGD55_15505"
     CDS             154..639
                     /locus_tag="VGD55_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871805.1"
                     /translation="MQFAATTSRLGSAIVSAAIAVAGVAGVTVGLVVQAPTALADDFV
                     PLPPNGVFAITGHGFGHGIGMSQYGAFGAAQQGLQAAQILNFYYPGTTQQQVGNPQIR
                     VHLSAYDSSGITMVAPPGQQMTVTDGATSATATGPTTLYKVQIDATAMHILFLDPTDQ
                     T"
     gene            712..2700
                     /locus_tag="VGD55_15510"
     CDS             712..2700
                     /locus_tag="VGD55_15510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY0871806.1"
                     /translation="MYNGADGTSRQYRGSIRIVRSAPTAAAAVNVLDMQSYLYGVVPR
                     EMPDSWALPAALQSQAIAARSYALSVSTPNSAWDICDTTACQVYGGQALVAANGTTTA
                     IEGVNASPAVDATNGIALYYQGAPAFTQFSSSNGGEVSPGSRPYLVAEADPYDTTAID
                     PNHDWTAQLSAATLQAAYPAVGTVQGLQVTSRDGAGDFGGRVLAVQLIGTGGTINVST
                     TLGLKSNYWTAGGGPSQIPVILNRGVAIGNLEAASSGGPGILNVAGWSFNNISKQVPD
                     FVDVYIDNTLAGRLPANLSRPDVGAAYGNVSPYHGFSGTFQTTGGAHSVCVKGIDGVA
                     NPTLRCMNATLPSGNPFGNLESVTDIGSGVQLTGWTIDPDTASPATVHVYVDGVWAGL
                     TTANVERDDVGAAYPGYSANHGFDIVVQAGQGKHTICAYAINIGIGTTNPLLRCATIT
                     LNRNPKGGVSLASVTSGVATVSGWAVDPDTHDPINVHLYVDGVWAGMTTANLDGATQA
                     DAAYPGYGTLHGYSISANLGAGSHSLCAYAINVGQGSTNPLLGCVQITGDGNPIGNFE
                     HLAKTSAGWVASGWTIDPDSNESIPVDVYVDGKLAIRVAADSGRADIAAIYAGWGAAH
                     GFNAPLALTAGKHQICAYGINTGPGTTNPSLGCLTVHV"
BASE COUNT          496 a          943 c          885 g          474 t
ORIGIN      
        1 acgaacgtgc cccgaattcc tgcactcgac acgatgaagg cggtcatgtt cgtcgtgtct
       61 gcccggccat tgtgatttct gaggttagtg tgacgaggct gacatcgatg tgggttctgc
      121 actgcggcga cccaccagcc ctctggagcc cgcgtgcagt ttgcagccac gacgtcccgt
      181 ctcgggagcg ccatcgtcag cgcggccatc gcggtcgcag gggtcgcggg tgtgaccgtc
      241 gggttggtgg tgcaggctcc gactgccctg gctgacgact tcgtgccgct gccgccgaac
      301 ggggtcttcg cgatcactgg ccacggcttc gggcacggca tcgggatgag tcagtacggc
      361 gctttcggtg ccgcgcaaca aggcctgcaa gccgcccaga tcctgaactt ctactacccc
      421 ggcaccacgc agcagcaggt cggcaacccg cagatccgcg tgcacctgtc ggcgtatgac
      481 tccagcggca tcacgatggt cgcgccgccc ggccagcaga tgacagtcac cgacggtgcg
      541 acgagcgcaa cggcgaccgg cccgacgacg ctgtacaagg tccagatcga cgcgaccgcg
      601 atgcacatcc tcttcctcga tccgaccgac cagacctgaa agccgttcgt cgtcggcgca
      661 accgctgaca ccgttggccc ggtcacgttc gcgacaccga gcggcgtccg catgtacaac
      721 ggcgccgacg gaacgtcgag gcagtaccgc ggctctattc gcatcgtgcg gagcgctcca
      781 accgctgccg ccgcagtgaa tgtgctcgac atgcagtcgt acctgtacgg cgtcgtgccg
      841 agggaaatgc ccgacagctg ggcgttgccg gcagcgctgc aatcgcaagc cattgctgcg
      901 cgttcgtacg cgctttcggt ctcgaccccg aactctgcgt gggacatctg cgataccacc
      961 gcgtgccagg tctacggcgg tcaagcactg gttgccgcga acggcacgac gaccgccatc
     1021 gagggtgtca acgcctcgcc cgcggtcgat gccaccaacg gcatcgcgct ttactaccaa
     1081 ggagcgccgg cgttcaccca gttctcatcg tcgaacggtg gcgaggtctc gccgggcagc
     1141 aggccctacc tcgttgccga agccgacccc tacgacacca cggcgatcga tcccaaccac
     1201 gactggacag cccagctctc cgccgccacg ctccaggccg cgtatccagc ggtcggcacg
     1261 gtgcagggcc ttcaagtcac gtcgcgtgac ggagctggtg atttcggcgg ccgggtcctc
     1321 gccgtgcagc tcatcggcac cggtggaact atcaacgtca gcacgaccct tggcctcaaa
     1381 tccaattatt ggacggcggg gggtggcccg agccagatac ccgtgatctt gaaccggggc
     1441 gtggcgatcg gaaacctcga ggcggcgtcg tctggtgggc cgggcatctt gaacgtcgcc
     1501 ggctggtcgt tcaacaacat cagcaagcag gtgcccgact tcgtcgacgt ctacatcgac
     1561 aacaccctcg cgggccggct gcctgcgaac ctctcgcgtc ccgacgtcgg cgcggcgtac
     1621 ggcaacgtca gcccgtatca cgggttcagc ggcacgttcc agacaacggg cggcgcgcat
     1681 tcggtgtgcg tgaagggcat cgacggcgtc gcgaacccga cgctgcgctg catgaacgcg
     1741 acgctgccca gcggcaaccc gttcggcaac cttgagtcgg tcaccgacat cggcagcggc
     1801 gtgcagttga cgggctggac catcgacccc gacaccgcga gccccgccac cgtgcatgtg
     1861 tacgtcgacg gcgtctgggc aggtctcaca acggcaaatg tcgagcggga cgacgtcggc
     1921 gccgcatatc ccggctatag cgcaaatcac gggttcgaca tcgtggtgca ggccgggcag
     1981 gggaagcaca cgatctgcgc gtatgccatc aacatcggca tcggcacgac gaacccgttg
     2041 cttcgttgcg cgacgatcac gctcaaccgc aacccgaagg gcggcgtgtc actggcgagt
     2101 gtcacgagcg gggttgcgac cgtctcgggc tgggcggtcg accccgacac gcacgacccc
     2161 atcaacgttc acctctacgt cgacggcgta tgggcgggca tgacgaccgc gaatcttgat
     2221 ggcgctacgc aggccgacgc ggcgtacccc ggttacggca ccctgcacgg gtacagcatc
     2281 tcggcgaacc tcggcgcagg cagccacagc ctgtgcgcgt acgcgatcaa cgtcgggcag
     2341 ggcagcacga acccgctgct tggttgcgtg cagatcaccg gtgacggcaa tccgatcggc
     2401 aacttcgaac acctggccaa gacaagcgcg ggttgggtgg cctcgggttg gacgatcgat
     2461 cccgatagca acgaatcgat tccggtggac gtctatgtcg atgggaagct ggccatccgg
     2521 gtcgctgccg actcagggcg ggctgacatt gcggcgatct acgcaggctg gggcgccgca
     2581 cacgggttca acgcgccgct cgctcttacc gccgggaagc accaaatctg tgcttatggc
     2641 atcaatactg gaccaggcac cacgaatcct tcgttagggt gccttaccgt tcacgtctga
     2701 cctggttgtg ccgggtcgct ttgattcacc gaggagatcc cgctgtgtcg caaattcgcc
     2761 gtcccgtcgt tctcggccta gcgaccgaac ttaccttc
//
LOCUS       DASWVF010000880         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_305434, whole genome shotgun sequence.
ACCESSION   DASWVF010000880 DASWVF010000000
VERSION     DASWVF010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305434"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGD55_15515"
     CDS             <1..354
                     /locus_tag="VGD55_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0871807.1"
                     /translation="HYMDEAEQLCDRIVVLDRGVIVAVGSPRELIDRHASHEVLELRF
                     EAGAEAVSAEKIRDAGIGDRIEILAARLLCSTDDGETALAAVHRLGIRPVNVVVRRST
                     LEDVFLSLTGRTLID"
     gene            356..1168
                     /locus_tag="VGD55_15520"
     CDS             356..1168
                     /locus_tag="VGD55_15520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871808.1"
                     /translation="MSAISATTRSGEPAVGTFLGRHEFAYRLQRFRRTWRGTVVISVV
                     NPLLFLIAIGLGLGHVVSHPAALNGVSYLAFFAPGMLAASAMQNGIVEAAFPVSHGVG
                     PGGSYRVAITTPLESTDIMLGHILFMTLKIAMSAAAFVAVMVAFGAATSAWVILTVPA
                     ATLTGLAFATPTAAFAATVSSPKVVGNLFKWVVMPLYLFSGTFFPVSQLPLALRALAY
                     ATPVWHGVDLCRTPSLGTATWGRCAVDVGYLVAVTALGVVWARYTYRRRLYG"
     gene            1161..1946
                     /locus_tag="VGD55_15525"
     CDS             1161..1946
                     /locus_tag="VGD55_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0871809.1"
                     /translation="MADLLVISSARQIVVRHWWVLRRGNPWTLLVNGLFEPFLYLMSI
                     GVGIGSLVGNHGRTIAGGYSYASFVAPALLATSAMNTATAETIWGLWYRMRFERFYDS
                     LITTPLTAYDIAVGEVVAATLRGLFAGSCFLGVIALLGMTHSMWAVLALAAVALSAFS
                     FAAAGLAAATLMRELHHNQYVQLVMLPMFLFSATFYPLSVYPTFVRDVVAALPLYQST
                     NLLRGLTLGQVGRGQLFATVYLLVFGFASLWFGSGRLKRLLGH"
     gene            complement(1964..>2429)
                     /locus_tag="VGD55_15530"
     CDS             complement(1964..>2429)
                     /locus_tag="VGD55_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0871810.1"
                     /translation="RAGARARDNSPMWPGCSDVRFVDMPGEHTAMGWPVDETGLHELL
                     LRLYREYGVPLVITENGAAYDDSVSADGEIHDAQRVSYLRRHLAAVHQAIADGVDLRG
                     YYVWSLMDNFEWAWGYDKRFGVVRVDFDTQERTIKDSGHFYRDVVRDNALPA"
BASE COUNT          361 a          809 c          757 g          502 t
ORIGIN      
        1 cactatatgg atgaagccga gcagttgtgc gatcgcatcg tcgtgctcga ccgcggagtg
       61 attgtcgccg tcggctcccc tcgcgagctg atcgacaggc atgcgtcgca cgaagtgctt
      121 gagcttcgat tcgaggcggg cgccgaagct gtgtccgccg aaaagatccg cgacgccggc
      181 atcggcgacc gcattgagat cctcgccgcg cggctgctgt gctcgaccga cgacggcgag
      241 accgcacttg ccgccgtcca tcgccttggt attcgtccgg tgaatgtggt tgtccgtcga
      301 tcgacgctcg aagacgtgtt cttgtccctc accggccgca cactgatcga ttgagatgtc
      361 cgctatcagc gccacgacca ggtccggcga accggccgtc ggcacattcc tcggccgcca
      421 cgagttcgcc taccgcttac agcgcttccg ccgcacctgg cgtggcaccg tcgtcatcag
      481 cgtcgtcaat ccgctgctgt tcctgatcgc catcggtctc ggtcttgggc atgtcgtcag
      541 ccatccggct gcgctgaacg gcgtgagcta cctggcattc ttcgctcccg gcatgctggc
      601 ggcctcggct atgcagaacg ggattgtcga ggccgcgttc cccgtcagcc atggtgtcgg
      661 gcccggtggc agctaccgcg tcgcgatcac cacaccgctc gaatcgaccg acatcatgct
      721 gggccacatc ctgttcatga cgctgaagat tgcgatgagc gccgctgcct tcgttgccgt
      781 gatggtggca ttcggtgcgg ccacatcggc gtgggtgatt ctcacggtgc ccgccgcgac
      841 cctgacgggc ctcgcgttcg cgacgccaac ggccgcgttc gccgcgaccg tttcgagccc
      901 gaaggtcgtc ggcaacttgt tcaagtgggt cgtgatgccg ctctacctgt tctccggcac
      961 gttcttcccc gtcagccaac tgccgctagc ccttcgcgcg ctcgcctacg ccacgccggt
     1021 gtggcacggc gtcgacttgt gccgcacccc gagcctcggc acggcaacat ggggtcgctg
     1081 cgcggtcgat gtcggttatc tggttgcggt gaccgcccta ggggtcgtct gggcgcgata
     1141 cacctaccgg aggcggctgt atggctgacc tgctggtgat ctcgtccgcg cggcagatcg
     1201 tcgtccggca ttggtgggta ctgaggcgtg gcaatccctg gaccctgctg gtcaacgggc
     1261 tcttcgagcc gttcctttat ctgatgtcga tcggtgtcgg catcggttcg ctcgtcggta
     1321 accacggccg cacgattgcc ggcggctaca gctacgcatc gttcgtggcg cccgcgctgc
     1381 tcgcgacatc ggcgatgaac accgcgaccg ccgagacgat ctggggcctg tggtaccgca
     1441 tgcgattcga gcggttctat gactcgctga tcacgacccc cctgaccgcg tacgacatcg
     1501 cggtcggtga agtcgtcgca gcgacgctcc gtgggctgtt cgcggggtcg tgttttctcg
     1561 gagtgatcgc gttgctcggg atgactcatt cgatgtgggc cgtgcttgcg ctggctgcgg
     1621 tggcactcag cgcgttctcg ttcgctgcgg ccggactcgc cgccgcaacg ctcatgcgcg
     1681 agctacacca caaccagtac gttcagctcg tcatgctgcc gatgttcttg ttctctgcga
     1741 cgttctatcc gctgtcggtc tatccgacct tcgttcgcga tgtggtcgcc gcgcttcctc
     1801 tttaccagag caccaatctg ctccgcggcc tcaccctcgg acaggtcggc agagggcagc
     1861 tcttcgcgac ggtctacctc cttgtgttcg gcttcgcgag cctgtggttt gggtcaggca
     1921 ggctgaagcg cctgctcggg cattgacgca accgaccccg cggctacgcc ggcagcgcgt
     1981 tgtctcgcac gacgtcgcga tagaaatgac cgctgtcctt gatggtccgc tcctgggtgt
     2041 cgaagtcgac gcggacgact ccaaagcgct tgtcgtaccc ccacgcccac tcgaagttgt
     2101 ccatcagcga ccagacgtag tacccacgaa ggtcgacgcc gtcagcaatt gcctgatgaa
     2161 cggccgccag gtgacgccgc aaataggaga cacgctgcgc gtcatggatt tcaccatcgg
     2221 cagaaacgga atcgtcatac gcggcgccgt tctcggtaat gacaagcggg acgccgtact
     2281 cgcggtacag gcgaagaagc agctcgtgaa gtccggtttc gtcgaccggc cagcccatcg
     2341 cggtgtgctc tccgggcatg tcgacgaatc gcacgtcgct gcatccgggc cacatcggcg
     2401 agttgtcccg agcgcgggct ccggcccgc
//
LOCUS       DASWVF010000881         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_50849, whole genome shotgun sequence.
ACCESSION   DASWVF010000881 DASWVF010000000
VERSION     DASWVF010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50849"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGD55_15535"
     CDS             <1..438
                     /locus_tag="VGD55_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0871811.1"
                     /translation="GGIMPVSAVVSTWDVMGALHPGEHGSTFGGNPLACAVGRAVVGL
                     LETGEFQTRAAVLGAQLADRLSALVGHGVVGFRSRGLWAGVDIDPAIATGRQVCEALV
                     GQGVLAKDTHGSTIRLAPPLVVTAADVDFAVDALTAVLADLTR"
     gene            501..1718
                     /locus_tag="VGD55_15540"
     CDS             501..1718
                     /locus_tag="VGD55_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871812.1"
                     /translation="MGANGFRRGLTPPRVSLALGSLTVVAVVAGCVLTVLAHDLRASF
                     DGVLLLIVATVAAVGLVVARRQPSNPIGWVLLVASAFFAAYAVAVLYVVLDYHRHGGR
                     LPLGRVALAAEPCWVPGFVLLGLAVALFPDGRLTSRRWWTPLIVYIGAGAWFWTVWSL
                     SQATVRIRPGFDVDQVGNYAGDPGGFSGAVAGIGWAAAPIVLVFLMAFVIRQAATWRH
                     ATGERRKQLTWLMCGGALSVISIYLVSTNNTSSAGRVITDIAAVGLTAIPLAIGVGIL
                     KYRLYEIDRLISRTLSYLAITGLLAGVFLGLIALTTSVLPFSSPVGVAASTLAAAALF
                     TPLRRRVQRLVDRRFNRARYDADSIIATFTIRLRDAVDLDTISQELRQAVNQAVAPAH
                     ASVWLTSGTPRHT"
     gene            complement(1787..2896)
                     /locus_tag="VGD55_15545"
     CDS             complement(1787..2896)
                     /locus_tag="VGD55_15545"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF009810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY0871813.1"
                     /translation="MTLEILAVQGIPAVIAGNDLASLIVTHAPALLDGDVLVVTSKIV
                     SKAEGQRFDIDRELAIDAETVRVVARRGDTRIVKTRHGFVMAAAGVDESNVTKGEVLV
                     LPVDPDASARRIRDGVRSLTGVNVGVVISDTFGRPWRNGVTDAAVGFAGLLAFEDLRG
                     TVDPYGNELHVTLTCTVDEIAAAADLVKGKVNGIPVAIVRGLSVVIDGESPAAQGVSN
                     IVRRIEDDLFSLGTRDVLRARVDTFEFGDEPVDPELITRALQAASMAARPVGWDLLRF
                     AMLAARDVPPSLQFEVPLTATTVIVPYLFAPPAAQDLLATGAAIENVLVALAIEQVGS
                     NWVWLDVASRPELAAIVGVEECWPVGLIAVGLPAV"
     gene            complement(2893..3900)
                     /gene="cofD"
                     /locus_tag="VGD55_15550"
     CDS             complement(2893..3900)
                     /gene="cofD"
                     /locus_tag="VGD55_15550"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY0871814.1"
                     /translation="MTDLETPSRRIVALAGGIGGARFLRGLLAALKADDPSAQVTVIG
                     NTGDDITLFGLRVCPDLDTVMYTLGGGINEEQGWGRADESFNVQGELGLYGSAVDGVG
                     PQWFGLGDRDIATHVLRTKWLAEGVPLTEVTRRLCERWLPGVRLLPMSDDTVETHVEL
                     DDPVEGVRTVHFQEWWVRLHAAVPARSILAVGADRARPAPGVLSAIADADVIVLPPSN
                     PVVSIGTILAVPGIREALVAASAPVVGVSPIIGGAAVRGMADACLQAIGVETSARAVA
                     EHYGAALLDGWLIDNVDAGTMQPPVDGIVMQSRPLLMTDIDATAAIARAVLALAADLN
                     P"
     gene            4239..4472
                     /locus_tag="VGD55_15555"
     CDS             4239..4472
                     /locus_tag="VGD55_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY0871815.1"
                     /translation="MDIEEELRWQERALCAQTDPETFFPEKGGSTREAKRICTTCAVR
                     SECLEYALGNDERFGIWGGLSERERRRLKRRVS"
     gene            4660..>5192
                     /locus_tag="VGD55_15560"
     CDS             4660..>5192
                     /locus_tag="VGD55_15560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0871816.1"
                     /translation="MLVAHDGARWLPTTLHAVKTQRRPVQRFVAVDTDSHDDSRELLE
                     RTVGASSVIHAPRETGFGAAVNLAISAFAGAPGLASTRADSGAVVEWTWLLHDDSAPS
                     SNALEALLELADEMPSAGVIGTKLRDWDNPSVLLEVGATVDRGGRRETWLEPGELDHG
                     QHDGDRDVLAVSTAGMLV"
BASE COUNT          838 a         1759 c         1690 g          905 t
ORIGIN      
        1 ggcggcatca tgccggtgtc tgctgtcgta tcgacctggg acgtgatggg cgcgctgcac
       61 cccggcgagc acggcagcac gttcggcggc aacccgctcg cgtgcgcggt cggacgagcc
      121 gttgtcgggc tgctcgagac cggcgagttc cagacccgcg cggcagtact cggcgcgcag
      181 ctcgctgacc gactttcggc gctcgtcggc cacggcgtcg tcggatttcg cagccgcggc
      241 ttgtgggctg gcgtcgacat cgacccggcg atcgcgaccg gccgtcaggt ctgcgaagcg
      301 cttgtggggc aaggggtcct cgcgaaggac actcacggtt cgacgatccg tctcgcgcct
      361 ccgcttgtgg tgacggccgc tgacgtcgac ttcgccgtcg atgcccttac cgccgtgctg
      421 gctgacctca cccgctgacc gcgcgggttg cccacaaggt ccgtaacgct gccggaacgg
      481 tagttgccta gatttggcgc atgggagcga acgggttcag gcgcgggctg acacctccac
      541 gggtgtcgct cgctttgggc agcctcaccg ttgttgccgt ggtcgcgggt tgtgtgctca
      601 cggtcctcgc gcacgacctg cgcgcgtcgt tcgacggtgt gctgctcctg atcgtcgcaa
      661 ccgtcgccgc ggtcgggctg gtcgtcgccc gtcgacagcc gtcgaacccg atcggctggg
      721 tgctgctcgt cgcctccgcg ttcttcgccg catacgccgt tgcggttctt tacgtcgtcc
      781 tcgactacca ccgacacggc ggccgattgc cgcttggtcg cgtcgcactg gccgccgagc
      841 cgtgctgggt gcccgggttc gtgctgctcg ggctcgcagt cgcgctcttt cccgacggcc
      901 gcctcacttc ccggcgttgg tggaccccgc tgattgtcta catcggtgct ggagcctggt
      961 tctggacagt ctggtcgctg tcgcaggcga cagtccgaat tcggccgggg ttcgacgtcg
     1021 accaggtcgg gaactatgcg ggggacccag ggggcttttc aggagctgtg gctgggatcg
     1081 gttgggctgc ggcaccgatc gttctggtgt tcctgatggc gttcgtcata cgtcaggcgg
     1141 cgacctggcg gcacgcaacg ggcgagcggc ggaagcaact gacgtggctg atgtgcggcg
     1201 gggccctgtc agtcatctcg atctaccttg tctcgaccaa caacacgagc agcgcggggc
     1261 gtgtcatcac cgacatcgcc gccgtcggcc tgaccgccat cccgctcgcg attggcgtcg
     1321 ggatcctgaa gtacaggttg tatgagatcg accggctgat cagccgcacg ctgtcgtacc
     1381 tcgccatcac gggcttgctg gccggcgtct ttctcggctt gatcgcgctg actacgagcg
     1441 tcctgccgtt ctcctccccc gtcggcgtcg cggcatcgac gctcgccgcg gcagcgctct
     1501 tcacgccatt gcgccgacgc gtgcaacggc tcgtcgaccg gcgcttcaac cgtgcccgct
     1561 acgacgccga ttcgatcatc gccaccttca caattcgact ccgcgacgcc gtcgacctcg
     1621 acacgatcag ccaagaactg cgccaggcgg tcaaccaagc cgttgcgccg gcacacgcct
     1681 ccgtgtggct cacgtcgggg acaccgagac acacctgatc gctttcggca gaccaactcg
     1741 ggccggtcgt cgacccgcac ccctgaagcg cgacaccaaa aagggctcag accgcaggta
     1801 gcccgaccgc gatgagtccc accggccagc actcctcgac gccgacgatg gctgcgagct
     1861 ccggccgcga ggcaacgtcg agccataccc agttcgagcc cacctgttcg atcgcgaggg
     1921 cgacaaggac gttctcgatg gctgcgcctg tggcgagcag atcctgcgcc gcgggcggcg
     1981 cgaacagata gggcacgatc accgttgttg cggtgagcgg tacttcgaac tgcaatgaag
     2041 gcgggacgtc gcgggcagcc agcatggcga agcgcaacaa gtcccacccg accggtcgag
     2101 cggccatcga ggcggcctgc agtgcgcggg tgatcagctc ggggtcgacg ggttcatcgc
     2161 cgaactcgaa agtatcgacc cgggcccgca gtacgtcgcg cgtgccgagg gagaacaagt
     2221 cgtcttcgat ccgccgcacg atgttagaaa ccccttgcgc agcagggctt tctccatcga
     2281 tcacaacgga caggccacgc acgatcgcga ctggaatccc gttcaccttg cccttgacga
     2341 ggtcggccgc cgctgcgatc tcgtcgactg tgcaggtgag cgtgacgtgc agttcgttgc
     2401 cgtacggatc gacggtcccg cgcaaatcct cgaacgcgag caggccggcg aacccgacgg
     2461 cagcatcggt gacgccgttg cgccacggac gcccgaacgt gtcgctgatg accaccccga
     2521 cgttcacgcc agtcagcgag cggacgccgt cgcggatccg ccgtgccgaa gcgtcagggt
     2581 cgacgggcag cacgagaacc tcgcccttcg tgacgttcga ctcgtcgacg ccggctgccg
     2641 ccatcacgaa tccgtggcgg gtcttcacga tgcgcgtgtc gccacgtcgc gcgactacgc
     2701 gaacggtctc ggcatcgatc gccaactccc gatcgatgtc gaaccgctga ccttccgcct
     2761 tcgacacgat cttcgaggtg acgacgagca cgtcgccgtc gagcagagcc ggggcgtgcg
     2821 tgacgatgag gctggcgagg tcgttgccgg caatcactgc gggaatccct tgcaccgcaa
     2881 ggatttctaa agtcacgggt tcaagtctgc cgcaagtgcg agcacggcgc gcgcgatggc
     2941 tgcggttgca tcgatgtcgg tcatcagcag cggccgtgat tgcatgacga tgccgtcaac
     3001 aggcggctgc atcgtcccgg catcgacatt gtcgatgagc cacccgtcaa gcagcgcagc
     3061 tccgtagtgc tcggcgaccg cgcgcgcact cgtctcgacg ccgatcgcct ggaggcacgc
     3121 atcggccata cccctgaccg ccgcgccgcc gatgatcggt gagaccccga cgaccggggc
     3181 cgaagcagcc accaacgcct cgcggatacc gggcacggcg aggatcgtgc cgatgctcac
     3241 caccgggttc gacggcggga gcacgatcac atcggcgtcc gcgatcgccg acagcacgcc
     3301 aggagctggc cgcgcacgat cggcgcccac cgcgagaatg ctgcgcgcgg gtactgctgc
     3361 gtgtagccgc acccaccact cctgaaaatg cacggtccgc acaccctcga ccggatcgtc
     3421 gagctcgaca tgcgtctcga ccgtgtcgtc agacatgggc agtagacgga cgccgggtag
     3481 ccaccgttcg cacaatcgcc gggttacctc ggtcagtggc acaccctcgg ccagccactt
     3541 cgtgcgcagc acatgagtcg cgatgtcccg atcgcccaag ccgaaccact gcggccctac
     3601 gccatcgacg gcgctgccgt acaaaccgag ctcgccctgc acgttgaacg attcgtcggc
     3661 acgaccccag ccctgttcct cgttgatgcc cccacccagg gtgtacatca cggtgtcgag
     3721 gtcgggacac acacgcaggc cgaacaacgt gatgtcgtca ccggtgttgc cgatgaccgt
     3781 cacctgggcc gacgggtcgt cggccttcag tgcagcgagc agtccgcgaa ggaagcgcgc
     3841 tcctccgatg ccaccggcga gcgcgacgat gcgtcgcgaa ggcgtttcaa ggtctgtcaa
     3901 ggtctgccac cgtcgcccac gagaaccgat cgttgcagac ccctgcgagg ccctctgacc
     3961 cggcttttgg tcggcgcaat accgcatcgg acctgctgcg ggcggctccc ttaccaggga
     4021 cttccgacat gttgcggcag cgaatatcac cgtttggctt gactagcggg taatgacgcg
     4081 catgtaattc cacctatgtt cttcgcaggc ggcgcattgg agccccgttt cgaaggcgat
     4141 gagggtcgag gaggtacgcg gtgatcgaga tctaccgcga ggtccacccg ctgcatgctg
     4201 acgccgaaga ggcgcaagca gctgagtccg gcttcccgat cgatatcgag gaagagctgc
     4261 gttggcagga acgcgcgctg tgcgcgcaga ccgatccgga gaccttcttc ccggagaagg
     4321 gcgggtcgac tcgcgaggcg aagcgcatct gcacaacctg cgcagtccgt tccgagtgcc
     4381 ttgaatatgc cctcggaaac gacgagcgct tcggtatctg gggtggtttg tccgagcgtg
     4441 agcgccgtcg tctcaagcgt cgcgtcagct aagtgagtgg agtaactgtc ccggtcggtg
     4501 atctcaagcc gaccgggtac cactgagccg acgaggtgcc actgtctgat cgcgtagggt
     4561 gaggcgtttc cgagttcatc cttctcccgg cctggccgta tgacctcatc tgccatcaga
     4621 aacgaccgtc tggtccgccc ccgtcacgtc gtcacagcgg tgctcgtcgc ccatgacggc
     4681 gctcgttggc tgcccacgac cttgcatgcc gtcaagaccc agcgccgccc ggtgcagcgg
     4741 ttcgtggcgg tcgacaccga cagccacgac gacagccgcg agttgcttga acggaccgtc
     4801 ggcgcgtcca gcgtcattca cgccccccgg gagaccggct tcggcgcggc ggtgaacctg
     4861 gcgatttcgg cgttcgccgg cgcaccggga ctcgcctcga cccgcgccga ctcgggtgct
     4921 gtggtcgagt ggacatggct gttgcacgac gacagcgcac cgtcgtccaa cgcactcgaa
     4981 gctttgctcg aactggccga cgagatgccc tccgccggtg tcatcggcac gaagctgcgc
     5041 gactgggaca acccgtcggt attgctcgag gttggcgcca ccgtcgaccg cggcggtcgt
     5101 cgcgagacgt ggctcgagcc gggcgagctg gatcacgggc agcacgatgg tgaccgtgat
     5161 gtgcttgcgg tgagcacggc cggcatgttg gt
//
LOCUS       DASWVF010000882         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U3622
            k141_178206, whole genome shotgun sequence.
ACCESSION   DASWVF010000882 DASWVF010000000
VERSION     DASWVF010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959434
            Sequence Read Archive: SRR11271807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 03:37:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,085
            Genes (coding)                    :: 3,067
            CDSs (with protein)               :: 3,067
            Genes (RNA)                       :: 32
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 6 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178206"
                     /isolate="SMAG_U3622"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="Chile"
                     /lat_lon="38.82 S 71.68 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..382)
                     /locus_tag="VGD55_15565"
     CDS             complement(23..382)
                     /locus_tag="VGD55_15565"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0871817.1"
                     /translation="MDAPTVSMDVRHVASGTAVVDIAGEVTSACEPVLMSAYEQISGR
                     DTQRVVLNFSGLEYMNSGGIGMLVTLLVRTNRQRQQLAAYGLSDHYRQIFELTRLDEA
                     ITIFDDETAALAGATTR"
     gene            complement(408..770)
                     /locus_tag="VGD55_15570"
     CDS             complement(408..770)
                     /locus_tag="VGD55_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0871818.1"
                     /translation="MPSSCEVDVQRVPGAVILVMRGEIDATAALPLQSAYAETTGPDM
                     APRVVLDFTAVDYMNSTGIALIVGILAQARTEHRTVAASGLSDHYRQIFEITRLSDFI
                     QVYADVDDAIATTAQPTA"
     gene            complement(754..1263)
                     /locus_tag="VGD55_15575"
     CDS             complement(754..1263)
                     /locus_tag="VGD55_15575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0871819.1"
                     /translation="MTSNQAQESVEVLATFAVPSQPGNERVALREVAAVTADRGLTPD
                     QMERLKTAVAEATMNAIEHGNENRPEVPVDITVVQTAGAIAVTITDRGGDRADNAPRE
                     IPDIELKLRGEQRPRGWGLFLVQAMVDGMEVSTQGALHTVRLTMQTGHTDSLNRPEGS
                     EGDDDAKQL"
     gene            complement(1260..2507)
                     /locus_tag="VGD55_15580"
     CDS             complement(1260..2507)
                     /locus_tag="VGD55_15580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0871820.1"
                     /translation="MTESLLSQDAAPDVDAVAARPAPAAPPPAAQNGGRSVLRRMRRT
                     RTVTVPEPRAPIHAGPAFDIAPNDPILGYCQSADGPVDITELVLDSPGLAAMRAAGVV
                     LVVPLVSSGELIGLLHLGPRLSERDYSTDDRHLLDSLAGHAAPAIRVGQLVLEQQVEA
                     RQRERIEQELKVAQLIQQQFLPKELPDLPSWTVAAFYRPARQVGGDFYDFISLRDGRV
                     MVVVGDVTDKGVPAALVMASTHALLRSTAPELTSPGAVLAHVNDLLCHDIPAHMFVTC
                     LALLLDPRSGKIEYANAGHDLPFLRTADGVVELKARGMPLGLMTGMEYEQKTTELHTG
                     DCLLLHSDGLAEAHNPKREMFGFPKIGEVVSRGDSGEALIDLCLSELDKFTGPNHEQE
                     DDITLVSLQRTGGSRFVGLEAGS"
     gene            2744..>2988
                     /locus_tag="VGD55_15585"
     CDS             2744..>2988
                     /locus_tag="VGD55_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0871821.1"
                     /translation="MPVLRLGLLGPPRIERDGSAVHFDTRKAVALLAVVSMAEQPLSR
                     DRLAAMFWPDSDDERARGALRRTLSVTGAGVGDALVVT"
BASE COUNT          459 a          947 c         1013 g          569 t
ORIGIN      
        1 cgatgtgttc atgattgcct cttcacctgg tggtcgcgcc cgcgagcgcg gcggtttcgt
       61 catcgaagat ggtgatcgcc tcgtcgagcc gggtgagctc gaagatctgc cggtagtggt
      121 ccgaaagccc gtaggcggcg agctgttggc gttgtcggtt cgtccgcacg agcaaggtca
      181 cgagcattcc gatgccgcca ctgttcatgt actcgagtcc actgaagttc aagacgactc
      241 gttgcgtgtc gcggccgctg atctgctcat acgccgacat cagcaccggc tcgcaggcgc
      301 tcgtcacctc gcccgcgatg tcgaccaccg ccgtgccgct cgcgacgtgg cgaacatcca
      361 tgctcacggt tggtgcgtcc atgatttccc tctcttgtgc gcggcggtca ggccgtcggc
      421 tgtgcggtgg tcgcgatggc gtcgtcgacg tcggcgtaga cctggatgaa atcggagagc
      481 cgcgtgatct cgaagatctg gcggtagtga tctgacagac cgctcgccgc gacggtgcgg
      541 tgctcggtgc gcgcctgcgc gaggatcccg acgatcaggg cgatcccggt cgagttcatg
      601 tagtcgaccg cggtgaaatc gaggacgacg cggggtgcca tgtctggtcc ggtggtctcc
      661 gcatacgccg actgcaaggg caatgccgcg gtggcatcga tctcgccgcg catgaccaaa
      721 atcacggcgc ccggaactcg ctggacgtct acttcacaac tgcttggcat cgtcgtctcc
      781 ttcactacct tcagggcggt tcaagctgtc ggtgtgcccg gtctgcatcg tcaggcgcac
      841 ggtgtgcaat gcgccttgcg tcgacacctc catgccgtcg accatcgcct gcaccaggaa
      901 caatccccac cctcgcggcc gttgctcccc gcgaagcttg agttcgatgt caggaatctc
      961 acgcggggcg ttgtcggcgc ggtcgccgcc acgatcggtg atcgtcactg cgatcgcgcc
     1021 cgccgtttgc accacggtga tgtcgacggg cacctcgggc cggttctcgt tgccgtgctc
     1081 gatggcgttc atcgtcgcct ccgccaccgc agtcttgagc cgttccatct ggtcgggcgt
     1141 cagtccccga tcggcggtga cggcagcgac ttcgcgcagt gctacccgct cgttgccggg
     1201 ctggctcggt acggcgaacg tcgcgagcac ctcgaccgat tcctgggctt ggttgcttgt
     1261 catgacccag cctcaaggcc tacaaaccgg gaaccgccgg tgcgctgcaa gctgacgagt
     1321 gtgatgtcgt cttcttgttc atggttgggt ccggtgaact tgtcgagctc agaaaggcac
     1381 aggtcgatca acgcctcgcc ggagtcacca cggctgacga cctcgccaat cttcggaaag
     1441 ccgaacattt cgcgcttcgg gttgtgcgct tccgcgagac cgtcgctgtg cagcagcaag
     1501 cagtcgccgg tgtggagttc ggttgttttt tgctcgtact ccatgccggt catgagaccg
     1561 agaggcatgc ctctcgcttt gagctcgacg acgccgtcgg ccgtgcgcaa gaagggtaag
     1621 tcgtggccgg cgttcgcgta ttcgatcttg ccgcttctcg ggtccagcag gagcgcgagg
     1681 caggtgacga acatgtgcgc cgggatgtcg tggcacagca gatcgttcac gtgagcgagc
     1741 acggcaccgg gagacgtgag ctcaggcgct gtcgagcgca gcaacgcatg agtgctcgcc
     1801 atcaccagcg ctgccggcac gcccttgtcg gtcacgtcgc cgacgaccac catgacgcgc
     1861 ccgtcgcgaa gtgagatgaa gtcgtagaag tcgccaccga cctggcgagc agggcggtag
     1921 aacgcggcga cggtccagct cggcaggtcc ggcagctcct tcggcaggaa ctgctgctgg
     1981 atcagctggg caactttgag ttcctgctcg atgcgctcgc gctgtcgggc ttccacctgc
     2041 tgttcgagca ctagctgacc gactctgatg gcgggggcgg cgtgcccggc cagtgagtcg
     2101 agcaggtgcc tgtcgtcggt ggagtaatcg cgctccgaca gccggggtcc aaggtgaagt
     2161 aacccgatga gttcgccgga ggaaacgagc gggacgacca gcaccacgcc agccgcgcgc
     2221 atcgcggcca ggccggggga gtccagcacc agctcggtga tgtcgacggg accgtcggcg
     2281 ctctggcagt agccgaggat cggatcgttg ggcgcgatgt cgaacgccgg cccggcgtgg
     2341 atcggtgcgc ggggttcagg cacggtcacc gtgcgggtgc gtcgcatccg gcgcaagacc
     2401 gaccggccgc cgttctgtgc cgccggtggt ggcgctgccg gcgcgggcct agctgcgacc
     2461 gcgtcgacgt cgggcgcggc atcttgcgac agcagggatt cggtcatgac gacctcactg
     2521 tcaagcaggc gctgggaagc gaccagcgta gggccggtgg catcggatcg gtgcgacttg
     2581 gcctcatcgc ccctccctac ttccttacgg ccgcaagctg gattggcggc ctttcgagga
     2641 ggagtcttct cgttcaccgt tccgaaagcg ttacggtttg cgccaagccg gaatgtctgt
     2701 cacagaaggg ccaatccgtg gtccccgaac ctgagcgtgg ggggtgcccg tgctgaggct
     2761 cggcctgctc ggtccgccgc gcatcgaacg cgacggctcg gcggttcact tcgacacccg
     2821 caaagcggtc gcgctgctcg ccgtggtgtc gatggccgag cagccgttga gccgcgaccg
     2881 gcttgcagcc atgttctggc ccgactccga cgacgaacgg gcgaggggtg cgttgcggcg
     2941 caccctctcg gtcacagggg cgggtgtcgg cgacgcgttg gtcgtgac
//
